LOCUS       DAZUGK010000001        68163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZUGK010000001 DAZUGK010000000
VERSION     DAZUGK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.001"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..662)
                     /gene="mcbR"
                     /locus_tag="ACHY5W_000001"
     CDS             complement(<1..662)
                     /gene="mcbR"
                     /locus_tag="ACHY5W_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4444980.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(860..1897)
                     /gene="curA"
                     /locus_tag="ACHY5W_000002"
     CDS             complement(860..1897)
                     /gene="curA"
                     /locus_tag="ACHY5W_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFS4444981.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVKSNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            2078..2596
                     /gene="mddA"
                     /locus_tag="ACHY5W_000003"
     CDS             2078..2596
                     /gene="mddA"
                     /locus_tag="ACHY5W_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFS4444982.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2593..3042
                     /gene="ydcZ"
                     /locus_tag="ACHY5W_000004"
     CDS             2593..3042
                     /gene="ydcZ"
                     /locus_tag="ACHY5W_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4444983.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(3047..3277)
                     /gene="ydcY"
                     /locus_tag="ACHY5W_000005"
                     /pseudo
     CDS             complement(3047..3277)
                     /gene="ydcY"
                     /locus_tag="ACHY5W_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(3363..3536)
                     /gene="ortT"
                     /locus_tag="ACHY5W_000006"
     CDS             complement(3363..3536)
                     /gene="ortT"
                     /locus_tag="ACHY5W_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFS4444984.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            3731..3826
                     /gene="yncL"
                     /locus_tag="ACHY5W_000007"
     CDS             3731..3826
                     /gene="yncL"
                     /locus_tag="ACHY5W_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFS4444985.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(3923..5347)
                     /gene="patD"
                     /locus_tag="ACHY5W_000008"
     CDS             complement(3923..5347)
                     /gene="patD"
                     /locus_tag="ACHY5W_000008"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFS4444986.1"
                     /translation="MQHNLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRISARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(5369..6163)
                     /gene="ydcV"
                     /locus_tag="ACHY5W_000009"
     CDS             complement(5369..6163)
                     /gene="ydcV"
                     /locus_tag="ACHY5W_000009"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4444987.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(6153..7094)
                     /gene="ydcU"
                     /locus_tag="ACHY5W_000010"
     CDS             complement(6153..7094)
                     /gene="ydcU"
                     /locus_tag="ACHY5W_000010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4444988.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLENIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWHMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(7095..8108)
                     /gene="ydcT"
                     /locus_tag="ACHY5W_000011"
     CDS             complement(7095..8108)
                     /gene="ydcT"
                     /locus_tag="ACHY5W_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4444989.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTSGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(8126..9271)
                     /gene="ydcS"
                     /locus_tag="ACHY5W_000012"
     CDS             complement(8126..9271)
                     /gene="ydcS"
                     /locus_tag="ACHY5W_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFS4444990.1"
                     /translation="MCKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(9517..10923)
                     /gene="ydcR"
                     /locus_tag="ACHY5W_000013"
     CDS             complement(9517..10923)
                     /gene="ydcR"
                     /locus_tag="ACHY5W_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4444991.1"
                     /translation="MKKYQQLAEQLREQIVTGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(11002..11418)
                     /gene="hicB"
                     /locus_tag="ACHY5W_000014"
     CDS             complement(11002..11418)
                     /gene="hicB"
                     /locus_tag="ACHY5W_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFS4444992.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(11464..11640)
                     /gene="hicA"
                     /locus_tag="ACHY5W_000015"
     CDS             complement(11464..11640)
                     /gene="hicA"
                     /locus_tag="ACHY5W_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFS4444993.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            11862..12092
                     /gene="yncJ"
                     /locus_tag="ACHY5W_000016"
     CDS             11862..12092
                     /gene="yncJ"
                     /locus_tag="ACHY5W_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFS4444994.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(12184..14145)
                     /gene="rlhA"
                     /locus_tag="ACHY5W_000017"
     CDS             complement(12184..14145)
                     /gene="rlhA"
                     /locus_tag="ACHY5W_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFS4444995.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(14218..14754)
                     /gene="sutR"
                     /locus_tag="ACHY5W_000018"
     CDS             complement(14218..14754)
                     /gene="sutR"
                     /locus_tag="ACHY5W_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFS4444996.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHIYR
                     NGGEQIVHFHSLIHYPRS"
     gene            14807..16018
                     /gene="ydcO"
                     /locus_tag="ACHY5W_000019"
     CDS             14807..16018
                     /gene="ydcO"
                     /locus_tag="ACHY5W_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFS4444997.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGLVIVIAQGDVVTTDVVFKPVLPSYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(16053..16721)
                     /gene="ydcL"
                     /locus_tag="ACHY5W_000020"
     CDS             complement(16053..16721)
                     /gene="ydcL"
                     /locus_tag="ACHY5W_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFS4444998.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(17025..17618)
                     /gene="tehB"
                     /locus_tag="ACHY5W_000021"
     CDS             complement(17025..17618)
                     /gene="tehB"
                     /locus_tag="ACHY5W_000021"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFS4444999.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(17615..18607)
                     /gene="tehA"
                     /locus_tag="ACHY5W_000022"
     CDS             complement(17615..18607)
                     /gene="tehA"
                     /locus_tag="ACHY5W_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFS4445000.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            18731..19711
                     /gene="ydcK"
                     /locus_tag="ACHY5W_000023"
     CDS             18731..19711
                     /gene="ydcK"
                     /locus_tag="ACHY5W_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFS4445001.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTNKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGISVLWGEVYATDNVWIDNSE
                     ISQGAHISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGDCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(19706..20242)
                     /gene="rimL"
                     /locus_tag="ACHY5W_000024"
     CDS             complement(19706..20242)
                     /gene="rimL"
                     /locus_tag="ACHY5W_000024"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFS4445002.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNSFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(20305..20529)
                     /gene="ydcH"
                     /locus_tag="ACHY5W_000025"
     CDS             complement(20305..20529)
                     /gene="ydcH"
                     /locus_tag="ACHY5W_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFS4445003.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(20669..22324)
                     /gene="opgD"
                     /locus_tag="ACHY5W_000026"
                     /gene_synonym="mdoD"
     CDS             complement(20669..22324)
                     /gene="opgD"
                     /locus_tag="ACHY5W_000026"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFS4445004.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(22549..23892)
                     /gene="ydcJ"
                     /locus_tag="ACHY5W_000027"
     CDS             complement(22549..23892)
                     /gene="ydcJ"
                     /locus_tag="ACHY5W_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4445005.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNEMQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRHCGLL"
     gene            24109..25032
                     /gene="ydcI"
                     /locus_tag="ACHY5W_000028"
     CDS             24109..25032
                     /gene="ydcI"
                     /locus_tag="ACHY5W_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4445006.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLNRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(25070..26710)
                     /gene="trg"
                     /locus_tag="ACHY5W_000029"
     CDS             complement(25070..26710)
                     /gene="trg"
                     /locus_tag="ACHY5W_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFS4445007.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSSLAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVLALVMTLIT
                     FMVLRQIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISMSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEQVSFATV"
     gene            27100..27249
                     /gene="hokB"
                     /locus_tag="ACHY5W_000030"
     CDS             27100..27249
                     /gene="hokB"
                     /locus_tag="ACHY5W_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFS4445008.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(27227..27295)
                     /locus_tag="ACHY5W_000031"
     CDS             complement(27227..27295)
                     /locus_tag="ACHY5W_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445009.1"
                     /translation="MNLLVRCTGKIPALALTWTCRP"
     gene            complement(27321..27494)
                     /locus_tag="ACHY5W_000032"
     CDS             complement(27321..27494)
                     /locus_tag="ACHY5W_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445010.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(27739..28269)
                     /gene="cybB"
                     /locus_tag="ACHY5W_000033"
     CDS             complement(27739..28269)
                     /gene="cybB"
                     /locus_tag="ACHY5W_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFS4445011.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            28458..29459
                     /gene="gap"
                     /locus_tag="ACHY5W_000034"
     CDS             28458..29459
                     /gene="gap"
                     /locus_tag="ACHY5W_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4445012.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(29501..30940)
                     /gene="aldA"
                     /locus_tag="ACHY5W_000035"
     CDS             complement(29501..30940)
                     /gene="aldA"
                     /locus_tag="ACHY5W_000035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFS4445013.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEDGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(31137..31937)
                     /gene="ydcF"
                     /locus_tag="ACHY5W_000036"
     CDS             complement(31137..31937)
                     /gene="ydcF"
                     /locus_tag="ACHY5W_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFS4445014.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQTPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLNGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(32209..36111)
                     /gene="hrpA"
                     /locus_tag="ACHY5W_000037"
     CDS             complement(32209..36111)
                     /gene="hrpA"
                     /locus_tag="ACHY5W_000037"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFS4445015.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVSGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKHVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTANGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVEKVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            36312..36917
                     /gene="azoR"
                     /locus_tag="ACHY5W_000038"
     CDS             36312..36917
                     /gene="azoR"
                     /locus_tag="ACHY5W_000038"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFS4445016.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(36971..38287)
                     /gene="ynbD"
                     /locus_tag="ACHY5W_000039"
     CDS             complement(36971..38287)
                     /gene="ynbD"
                     /locus_tag="ACHY5W_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFS4445017.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVS
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGFGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRETKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYEHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(38277..40034)
                     /gene="ynbC"
                     /locus_tag="ACHY5W_000040"
     CDS             complement(38277..40034)
                     /gene="ynbC"
                     /locus_tag="ACHY5W_000040"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFS4445018.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLLLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDQLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(40050..40946)
                     /gene="ynbB"
                     /locus_tag="ACHY5W_000041"
     CDS             complement(40050..40946)
                     /gene="ynbB"
                     /locus_tag="ACHY5W_000041"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4445019.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILTTWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(40946..41551)
                     /gene="ynbA"
                     /locus_tag="ACHY5W_000042"
     CDS             complement(40946..41551)
                     /gene="ynbA"
                     /locus_tag="ACHY5W_000042"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4445020.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWGIASILLIWTAINRCRSVLLMSAER"
     gene            complement(41721..44027)
                     /gene="ydbD"
                     /locus_tag="ACHY5W_000043"
     CDS             complement(41721..44027)
                     /gene="ydbD"
                     /locus_tag="ACHY5W_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFS4445021.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINNEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(44091..44951)
                     /gene="pdxI"
                     /locus_tag="ACHY5W_000044"
     CDS             complement(44091..44951)
                     /gene="pdxI"
                     /locus_tag="ACHY5W_000044"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFS4445022.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALAREGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(45183..45773)
                     /gene="paaY"
                     /locus_tag="ACHY5W_000045"
     CDS             complement(45183..45773)
                     /gene="paaY"
                     /locus_tag="ACHY5W_000045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFS4445023.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVSASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(45755..46705)
                     /gene="paaX"
                     /locus_tag="ACHY5W_000046"
     CDS             complement(45755..46705)
                     /gene="paaX"
                     /locus_tag="ACHY5W_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFS4445024.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(46806..47060)
                     /locus_tag="ACHY5W_000047"
                     /pseudo
     CDS             complement(46806..47060)
                     /locus_tag="ACHY5W_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(47033..47791)
                     /gene="paaF"
                     /locus_tag="ACHY5W_000048"
     CDS             complement(47033..47791)
                     /gene="paaF"
                     /locus_tag="ACHY5W_000048"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFS4445025.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFYRRITNWR"
     gene            complement(47788..48858)
                     /gene="paaE"
                     /locus_tag="ACHY5W_000049"
     CDS             complement(47788..48858)
                     /gene="paaE"
                     /locus_tag="ACHY5W_000049"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFS4445026.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAEAALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(48866..49363)
                     /gene="paaD"
                     /locus_tag="ACHY5W_000050"
     CDS             complement(48866..49363)
                     /gene="paaD"
                     /locus_tag="ACHY5W_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFS4445027.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(49378..50123)
                     /gene="paaC"
                     /locus_tag="ACHY5W_000051"
                     /pseudo
     CDS             complement(49378..50123)
                     /gene="paaC"
                     /locus_tag="ACHY5W_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
     gene            complement(50132..50419)
                     /gene="paaB"
                     /locus_tag="ACHY5W_000052"
     CDS             complement(50132..50419)
                     /gene="paaB"
                     /locus_tag="ACHY5W_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFS4445028.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(50431..51360)
                     /gene="paaA"
                     /locus_tag="ACHY5W_000053"
     CDS             complement(50431..51360)
                     /gene="paaA"
                     /locus_tag="ACHY5W_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFS4445029.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKSWEEGTWVREAALAHAQKQHACKVA"
     gene            51645..53690
                     /gene="paaZ"
                     /locus_tag="ACHY5W_000054"
     CDS             51645..53690
                     /gene="paaZ"
                     /locus_tag="ACHY5W_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFS4445030.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            53938..56211
                     /gene="tynA"
                     /locus_tag="ACHY5W_000055"
     CDS             53938..56211
                     /gene="tynA"
                     /locus_tag="ACHY5W_000055"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFS4445031.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKSVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFTAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTTGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(56270..57769)
                     /gene="feaB"
                     /locus_tag="ACHY5W_000056"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /pseudo
     CDS             complement(56270..57769)
                     /gene="feaB"
                     /locus_tag="ACHY5W_000056"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
     gene            58005..58910
                     /gene="feaR"
                     /locus_tag="ACHY5W_000057"
                     /pseudo
     CDS             58005..58910
                     /gene="feaR"
                     /locus_tag="ACHY5W_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
     gene            complement(59083..59412)
                     /gene="ydbL"
                     /locus_tag="ACHY5W_000058"
     CDS             complement(59083..59412)
                     /gene="ydbL"
                     /locus_tag="ACHY5W_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFS4445032.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(59417..59602)
                     /gene="ynbE"
                     /locus_tag="ACHY5W_000059"
     CDS             complement(59417..59602)
                     /gene="ynbE"
                     /locus_tag="ACHY5W_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFS4445033.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEGLLETRSDLF"
     gene            complement(59599..62238)
                     /gene="ydbH"
                     /locus_tag="ACHY5W_000060"
     CDS             complement(59599..62238)
                     /gene="ydbH"
                     /locus_tag="ACHY5W_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFS4445034.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAVPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            62446..63435
                     /gene="ldhA"
                     /locus_tag="ACHY5W_000061"
     CDS             62446..63435
                     /gene="ldhA"
                     /locus_tag="ACHY5W_000061"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS4445035.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            63546..63968
                     /gene="hslJ"
                     /locus_tag="ACHY5W_000062"
     CDS             63546..63968
                     /gene="hslJ"
                     /locus_tag="ACHY5W_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFS4445036.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(63965..64231)
                     /gene="ydbJ"
                     /locus_tag="ACHY5W_000063"
     CDS             complement(63965..64231)
                     /gene="ydbJ"
                     /locus_tag="ACHY5W_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFS4445037.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            64505..68029
                     /gene="nifJ"
                     /locus_tag="ACHY5W_000064"
     CDS             64505..68029
                     /gene="nifJ"
                     /locus_tag="ACHY5W_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4445038.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATTPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        17206 a        17799 c        16257 g        16901 t
ORIGIN      
        1 cgattgtatt gctggtataa aatagcaata ttttgttgca taatttcagc aagacaatgt
       61 ttgcatccct ctttgtcttt tgcttttaac gcggcgagta atagacgata gttttctata
      121 cgctcccgta attccgctgg attaatcgct tcatagagat aatgtaaacc aggtcccatc
      181 ctgacccaca gttgctcaat catctcacac aggatgggca tatttgagcg atgataaatc
      241 gctaagcgaa atagcctgtt tacattaatg atttgttcca tgtcgccctt ttcttgcgcc
      301 tgctgtaatt tctcgagcaa ttcctggagt tccgccaggt cttgcggggt gaggttttca
      361 acagccagag caactgccat taattccagt tcgtagcgga tccgattgat ttcgtctaat
      421 tgacgtttcc ccacttccgg aactgtaaat gcttgcgctg gtgcgacaga aagcgcattc
      481 accgaaacca gacgtaataa tgcttcacgc acaggtgtaa tactcatacc taattgctcc
      541 gccagatttt tagtaatcag gcgtgcgcca ggttttaatg cgccaatact aagctgatgt
      601 ttcaggtcgt tctcaacctg cagagtcaaa ctgacatgtt tcatttttcc cgttccaggc
      661 attttaattc cttcgcgtgt tttctgtcca accctgcctg catggcagaa ggagttcacc
      721 tggtgtatta aagtgaataa tcataaacca ctcacctttg ttgttattag cagcagaaca
      781 atgacgtggt tagttaatta aagctgaaaa taagcttttt gcctggattt ttatggcggc
      841 ttacgccgcc gttgaaactt taatcatcac ccgccacgcg gatcaccact ttgccgaagt
      901 ttttaccctt cagcaggccg ataaacgtct gtggcgcatt ctctaaaccg tcagtaattt
      961 cttcgcggta gtggatttta tcctctttca cccattgccc catctccttc tgaaactcat
     1021 ggatgcggtg accataatcc tgagcgataa taaacccttg caagcgaata cgttttttca
     1081 gcaccgttgc catcaacagg ggtaaacgat ccggaccggg tggtagctct gtagcgttat
     1141 agctgctcac taagccacaa acgggaatgc gcgcagatgt attaagtaat ggtagcactg
     1201 catcaaatac cttaccgccc acgttttcat aatagatatc aatacctttt gggcacgctt
     1261 tcgccagttg ttcggcaaaa tcgtccgcgt ggtgatcaag gcaaacatcg aagcctaaca
     1321 cctcggtagc atggcggcat ttttccgcgc caccggctac ccccaccact ctgcaacctt
     1381 taagtttgcc gatttgcccc accgtcgccc ccactggtcc tgtcgccgca gctaccacca
     1441 acgtttcgcc ctctttaggc tgaccgatat ccagcagccc catataagcg gtaaagcctg
     1501 gcatccccag cacacccagc gaccacgatg gattttgcgg atgatcgcca agtttcacca
     1561 gatcatcacc actggatatg tcatagtcct gccatccact gtagcccagc acccagtcgc
     1621 caggctgata atcaggatga ttcgacttca cgacacggct caccgtaccg ccgaccatca
     1681 cgccgccaat atcaaccggc ggtgaataag atggctcatc gctcatacgt ccacgcatat
     1741 acgggtccag ggacaaataa actgtgcgca gtaacacctg tccttcaccc ggtgtggcga
     1801 catcatcttc ttcaagacgg aaattctccg gaaccggtgc gccatgtgga cgcgaggcca
     1861 gaacccaacg tcgattacgc tgcttttgtt gccccataat gctctcctgt gcttttggca
     1921 tgaatccatc aatagtagca ggctcacggg attggttttg tttgcagccg tcacatcccc
     1981 tctgcaacac gcaataattt catcgaggaa tccgttgctt cgggctgttt tcgatagtat
     2041 tgataacatc atcatcatta ttttattgag gccagacatg tccatccgtt ttgcccgcaa
     2101 agccgactgt gctgccattg cggaaattta taaccacgcc gtgttgtata cggcggctat
     2161 ctggaatgac caaacggtgg atgcagataa ccgcattgcc tggtttgaag cgcggaccat
     2221 agcaggttat ccggtgctgg taagtgaaga agatggcgta gtgacgggat atgcctcgtt
     2281 tggcgactgg cgtagtttcg atggttttcg ccataccgtg gaacattcgg tttatgtcca
     2341 tcccgatcat cagggcaaag gtctggggcg taaattgtta agccgattga ttgatgaagc
     2401 gcgggattgc gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcctcgct
     2461 gcatctgcac cagtcgctgg gatttgtcgt caccgcacaa atgccgcagg tgggcactaa
     2521 atttgggcgc tggctggatc tgacatttat gcagttgcaa ctcgacgaac gcactgaacc
     2581 ggacgcgatt ggatgaatca gtcgctcacc cttgcctttt tgattgccgc cggaattggt
     2641 ctggtagtcc agaacacgct gatggtgcgc atcacccaga cctcctctac cattctcatc
     2701 gccatgttgc tgaactcact ggtggggatt gtgctctttg tttctatttt atggtttaaa
     2761 caaggtatgg cggggtttgg cgaactggtg tccagcgtgc gctggtggac acttatacct
     2821 ggcttgctgg gatcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg
     2881 gcgacaacta ttgcagtact ggtcgccagc cagctgattg gcggtctggt tctggatatc
     2941 ttccgcagtc atggcgtacc actacgggcg ttgttcggcc caatttgtgg cgcaatttta
     3001 cttgtggttg gcgcctggtt ggtagccaga cgctcatttt aatccacagg atggtgccgc
     3061 ctttggttaa ctgttcatgg cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag
     3121 caatagcggt gcgcaaacgc tgctgctggg tgtagcgatc ttcccgactt aactccgcgt
     3181 catcgctcag ggcgattaat aattcgttca tatgggcaat gacgctctct tcaatcgcgg
     3241 catccacgcg cgcgatgact tcatccagat gcgacatact ttctccttag ccacttgtga
     3301 aatttatcga ttcatccaaa tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag
     3361 atttaaaaca gtgaaaacaa cagcgccacc gggaaactca tcggccatgt tgcccccacc
     3421 agaaaggcgc ttaagaaacg gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga
     3481 aatgcgattg ctgccataac cgcataaaaa acaagcatgt gttgatagag agacatgtac
     3541 gggcgtccag ccagatgaat aaaacgcgcg caaatatgcg tcattttttg acgtgcatca
     3601 agttttttaa ccggtttgag aaaaaaacat caaacagcaa cttgccctta tccatcttca
     3661 gaatgagtat tattgcggat tttcttaacc cgtactatac acataggggc aatgataaaa
     3721 ggtggcaaaa atgaatgttt ccagtagaac tgtagtactg ataaatgtct ttgctgctgt
     3781 tggtttgttt actcttatct ctatgagatt tggctggttt atttgatgta gtgattttgc
     3841 acgcagcacg tactgtcaga gaaatcagtg ctctgacagt acgcaagatc atctcatact
     3901 cttacgatct gcaatattat ccttaatgtt taaccatgac gtggcggacg acggtgtaat
     3961 cctccagccc ataaagtgac atatccttgc cgtaaccaga aagtttctgc ccaccgtgcg
     4021 gcatttcact taccagcatg aaatgggtat tgacccaggt acaaccatat tgcagccgtg
     4081 cgctgatgcg atgcgccctg cccacatctt tcgtccatac cgaagatgca agtccgtact
     4141 ggctgtcatt cgcccagttt accacctgtt cttcgttgtc gaagagcgta acactcacta
     4201 ctggaccaaa tacctctttt tgcacgatgg catcgtcctg taatgcgcca gccagcagcg
     4261 tcggcgcata gtaatagcca ttacccttgc gcttttcacc gccagtgatc actttgatgt
     4321 gccctgtcgc tttcgcctct tctactgcct tgctgacgcg ttcgagatgc gccagcgagc
     4381 ttaaaggtcc aagctccgta gactcgtcat ccggcgcacc agattttaac gttgccactg
     4441 cagcaccgag tttttccacc agcgtatcgt aaatgccttt ttgcgcgtag atccgacaag
     4501 ccgcagtaca atcctgtccg gcattgtaat agccaaatgt acgtacacct tcgaccactg
     4561 cttcaatatc cgcatcatca aaaacaatca ctggcgcttt gccaccaagt tccatatgag
     4621 tacgcttaat ggacggcgca gtatggctga tgatgtgttc cccagtggcg atagagcccg
     4681 tcagcgacac catccgcact ttgggatgac cggtcagcgg atcgcccacc gttttgcctc
     4741 tgccaaacag cacattaatc acgcctgccg ggaagatatc tttcgccagc tctgccaact
     4801 tcaacgcggt cagcggggta atttctgatg gtttaagcac tacgcagttc cctgccgcca
     4861 gcgccggagc cagtttccac gctgccatca tcagcggata attccacggt gcgatagaag
     4921 ccacgacccc caacggatcg cgacggatca tcgaagtatg accttcaaga tattcacctg
     4981 ccgccagacc attcagacag cgcgccgcac ccgcgaaaaa gcgaaaaaca tcgacaatcg
     5041 ccgggatttc atcattgaac gcactatgca gcggtttgcc acaattacgg gactccagtt
     5101 cggcaaaaac ctgaccattt tcttcgataa catcagccag tttcagcaga cattccgcac
     5161 gcactttcgg cgtggtttgc ccccattcgg caaatgctgc atctgccgcg cgcacagcag
     5221 catcgacctg ctctgcggat gcctcggcaa tctccagtaa aacgtccccc gttgccggat
     5281 tatagacagg ctgtttttcc ccttcgccac taaccagttc tccgttaatc agtaaattat
     5341 gttgcatagc attttcctgt atcagtggtt attgaccatt gtcgccttcg cgggttagcc
     5401 accaggcccc caggatcggc aaggttgtta ccaacataac cagcagtgcc accacgttgg
     5461 ttaccggtac atcacgcggt cgcccaagct gattgagcaa ccacaacggt aacgttcgtt
     5521 catgacctgc cgtaaaggtc gtaacgatga tttcatcgaa cgacaaggca aacgccagca
     5581 ttcctcctgc cagtaacgcc gaactgagat tcggcaacac tacgtagcgg aaggtttgcc
     5641 agccattggc cccaagatcc attgacgcct caaccagact ccatgaggtg cggcgaaaac
     5701 gggcgatgac attgttaaac accacaacta cacaaaaagt cgcatgaccg accacgatgg
     5761 tgaaaaatcc cggctccaga ttgatggttt taaaggcggt taataacgcc agaccagtga
     5821 caatgcccgg cagcgcaatg ggcagcagca gtaacagcga aatggcgttt ttgccgaaaa
     5881 agtctcgtcg ccacagcgcg gcagctgcca gcgtccctaa caccagcgcc attaatgtcg
     5941 ccagcgccgc cactttaagt gacagtgtca cggcatcaag aatatcacta cgctgtgctg
     6001 ccacgctaaa ccagcgcagc gtcaggccct gcggtggaaa actaaacgcc gcatcttcag
     6061 tgttaaacgc ataggcggcg attatcagga tgggaaaatg taggaaaaca acgccgcccc
     6121 aggccgccag tttgaggaaa aacggtgcgc gttcagagtg catcgaacgc tcccagacgt
     6181 ttcacgaacg ccaggtacag tgcgataaga acaatcggca ccagggtgaa tgccgccgcc
     6241 atcggcatat tgccaatcgc cccctgctgg gaataaacca tattgccgat aaaatatcct
     6301 ggcgggccaa ccagctgcgg gacgataaaa tcgcccagtg tgagtgagaa ggtaaagata
     6361 gagccagcgg caatacccgg gatcgccagc ggcaacacca catagcgaaa ggtttgtcgt
     6421 ggacgtgcgc cgagatcagc cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc
     6481 gcctgcacgg gcaggatcat gaacggcagc cagatataga gaaacaccag aaagcgcccc
     6541 agcccggaag ttgacagcgt attgccgccc accgcaggta atgtaaggaa cgcagtcagc
     6601 agtggttcca gcccaagatg ttgtaaaaac cactgagcca cgccatcttt tgccagtaat
     6661 aacgtccagg cataggcttt aacaatgtag ctcgcccaca tcggcaacat taccgcgata
     6721 taaaaaaacg ctttcatttt tccgctggta tagcgcgcca tatgccatgc cattggaaaa
     6781 gccagaatgg cgctggcgat agtgactgcc actgccatag tcagcgtgcg gagaatgatg
     6841 tcgtaattcg ccggattaaa cagcgcacgg atattctcca gcgtcagttc cggcgttacc
     6901 gacatggtga aatcgtcaaa agtataaaat ccctgccata acagtgtcag cagcgagccg
     6961 aaatagacaa tgccaaacca cattagcggg ccaagcagca gtaaaaacag ccccagcccc
     7021 ggattacgcc agaagaaacc ggacacctta cccagacctg gacgtgaagg tgattgcaat
     7081 acgttcatcg ccattcacct ctcctcaacc agaggcacca tcacatcacg cgaccaggaa
     7141 accatcacct gttgtccggg cgtgagcgtg gcaggcagtt cttcacctgt catattggcc
     7201 tgactcacca gcagtttttc accgccgctc agtgtcagtt caaaacgggt cgctgcgccc
     7261 tgatattgca ccgcctggat cgtgccatta gcctgcattt cgccggaggt attgagacgg
     7321 atatgttccg gccgcagggc gaagcttccc gtcatgccac aaagtttctc tgccatcagt
     7381 ccatcaaaaa cattcgatgt accaacgaac ccggcaacaa acggcgtgcg cgggcgcata
     7441 tagagatcgc gcggggaatc gacctgctca atgcgtccat tattgaaaac cgccacacga
     7501 tcggacatcg ataacgcttc gccctgatcg tgggtaacga agataaaagt gataccgaga
     7561 gactgttgca gctttttcag ttccagctgc atctgctcac gcaatttgag atccagtgcg
     7621 ccgagcggtt catccaacag caatacgcgc ggttcattca ccaatgctct ggcgatagca
     7681 acccgctggc gctgaccacc agaaagttgt gacggtttgc gttgctgaac aaaacccagc
     7741 gccactttct ccagcgcctc ttgcgccatt gcgtgccgct gctttttatt cacgcctttg
     7801 accatcagcc cataggcgac attgtcaaga atcgacatat gcggaaatag cgcgtagtcc
     7861 tgaaagacag tattcacgtc ccgctcccac ggtggcagat tgctggcggg tttaccaaag
     7921 atagagatag ccccgccaga aagctgttcg aagccagcaa tcaggcgcag gcaggtggtt
     7981 ttgccggagc cggacggccc cagcatagag aagaactcac catcttttat cgcaatactg
     8041 acgccatcta ctgcgcgcac gtcaccgtac aaccgcgaga cgttgtcaaa ctccactgcg
     8101 tacgtcataa agcaccccag cgaagttagc gaccgcccat aatggcaatg taatcctgcg
     8161 tccagcgact gtagggaaca aacttgcccc cttctgctat aggcgttttc cagaaggcta
     8221 ttttatcgaa atagttaaaa ccgttggttt cacaaccttt ttcgcctaat aacggactgg
     8281 ctttacaccc ttgcggcact accggtaacg agccaaacca ggccgccaca tcgccctgca
     8341 cttttggcgt taatgaccag ttcatccatt tgtaggcgca aaccggatgt ttcgcttcgc
     8401 tatgtagcat ggtggtgtca gcccaaccgg taacgccctc cttcgggaaa acggtagcga
     8461 caggctggcc ttcggctttc aaggcgttgg cctgataggg ccatgcactg gaagcaacca
     8521 caccctcgtt tttgaaatcg ctcatttgca cggtagtgtc atgccagtag cgatggatca
     8581 aactatgttg agcgcgcagc actttcagca ccgcctggta ctgttcttcg gtgagttgat
     8641 acggatcgct gatgcccaac tgcggctgag tggctttaac gaacaacgca gcgtccgcaa
     8701 tgtagatagg gccatcataa gcctgaacgc ggcctttatt gctcttgccg tccggcagat
     8761 tttgctcaac aaaaaccact tgccagctat ccggcggcgt cgggaaggtt ttagtgttgt
     8821 acatcagcag gttcggaccc cattgatatg gcgtgccata gactttgccg cccacgttaa
     8881 accagtcgcc tttaaccacg cgcggatcga gcgttttcca gttgggaatc aatgcggtat
     8941 taatcggctg cacgcgttta cccataatca aacgcagcga ggcatcgccg gatgccgtaa
     9001 ccagatcgta accccctttg gtcatcagac tgaccatttc atcggaagtc gcggcggttt
     9061 taacattcac cgcgcagcct gtctcttttt cgaattgcgt tacccagtcg tattgtttat
     9121 cagtttgtcc gcgttcgata tatcccggcc aggcgataat atccagtctc ccttccggtt
     9181 tatctaaatt ggtaggtggt tcggcggcgt gagcggtcat tattgtcatg ctgagcgcac
     9241 acaggctgct gcgggcaaat gtcttgcaca taaggttctt actcctgtcg taattaattg
     9301 ttcagccgcc gtgccgtaaa aatatctcct tgtttaacca tagacggcgg cttgcaggca
     9361 caccttcgcc cctgagaaaa tttaatcagg ttgtgagcta ccgcgcatta tgccgtctgt
     9421 aaggttatct tctggagtat agacaaggag ttaggaaata tgactgctat tcgaaattca
     9481 tattacccaa cctaagagga attaaattaa acgctattac agccgttctt gaataagttt
     9541 gcctaattgt tttaccgcct gttcttcacg ttctccccac tgccacgcgg tattaaaacg
     9601 aaaaaaacgt gaccagtttt caccggtaga aaacatttta cccggcgcaa tactgatatg
     9661 atgcgtcagt gccgccaggc tcaattcgcc ggcatctaac ggctcgggga gctccagcca
     9721 gagaaaataa ccgctgtcat tatgatgaat tttcacttcc gcaggcagat aacgcagcag
     9781 tgcctgccag gcacgttgtt tacgttccgc aagctggcga cgcaggcgac gaagatgggc
     9841 gtcgtatcgg cgcgtggaaa ggtaatccac cagcgcaagc tgcatcggtg agctggtgga
     9901 aagcgtactc atcaactgca atcgttgaat tttacgtgca tgttttccgg cggcgaccca
     9961 accaatacga aaaccaggca ccagacattt cgaaaacgaa gagcaatgca aaacgccatc
    10021 gtggcgatcc cacgctttcg caggcagcgg tttttcccgt ccaaaataaa gttcgctgta
    10081 aacgtcatct tcaatcagcg ttacgttgta ctgattgagc aacgccacca gttgtgcttt
    10141 tttttgcggc gttaaggtaa atccgagtgg attctggcta ttagtcatca gccagcacgc
    10201 tttcaccgga tactcctgca acgccagttc cagcgcctga agatctatcc cttctttaac
    10261 atccgtcgct accgataacg ctttcagccg tagccgttcc agcgcctgca acgcgccata
    10321 gaaacaagga ttctctacta tcacccaatc gcccggttca gttaccgctt gcaaactgag
    10381 gtttaatgct tctaacgccc cggcagtgat gacaatttca tcaggagaaa tggtgatgcc
    10441 ctgtaaggca tagcgacggg caatagcctg acgcagttct gcgtttcctg gcggcaagtt
    10501 ttcaatcacg ctcatcgccg tggcggtttt gcttacctgc gccagcgagc ggtttagttg
    10561 ttggaggggg aaaagtcgcg ggtcgggaaa ggccgaggca aacggaacga ccgacggatc
    10621 gcgactggcc tgcaacatat caaaaatata agtgttgata tcgactgctt catctcgagt
    10681 gactggaatg actggcgctt tcggcatttt tattgcctgt ggcgcaacgt aataacccga
    10741 ctgcggtcgt gcgataatat atccctgact ttcgagcaac tgataggcat ggctgacagt
    10801 cataaagctc atgccagaaa gcgccacctg gtcacgcaac gaaggcaaac gatcgccggg
    10861 ttgccagata cccgtcacaa tctgctcgcg taattgttct gcaagctgct ggtatttttt
    10921 cattatcgaa tcgtaattat gtgcagatga ttcggcagtc tatatcagtt gttaaaaatg
    10981 acaacttttc attaactgtt atcaaaccat caccagtgat aactctttgc caagcgcctt
    11041 agccgcgagc tggacggcgt cgatttttgt cgcatggtgc aagttaaata agcgagtaat
    11101 ttcctgttta ggtttgccaa ttcgcctggc taactcttgc tgagtgattt ctgactgtaa
    11161 aaaagcattt aacagcaata ccttagaggc gacgctcaaa ggtacttcaa taaaatgatc
    11221 gtgactattt aatggcgaag gtaacgggat aagctcgtta tcttcaaaat aaaaatcaaa
    11281 tgcggtcagt aaagcatctt ttgccgcttc catcgcttca gcgacagttt cgccctgggt
    11341 caacgcttca gggatatcca caaaagaaac catataaccg ccttccggcg cgggtgtaag
    11401 agtgacggga taacgcataa tttcgactgt ctctcgcatg tcattaacct ttttaattgg
    11461 cgattaactc aaaccgagtt gtttcaggat tgctttacgc aatggttctt taatctcatc
    11521 gcaggggtga cgcggcatga cactgcgcct cccatgaaac ctgagtttca aatggttgct
    11581 gccattcgct acatcgacgc cctgagattc gagccaacgt ctgaactcgc tttgtttcac
    11641 atcgcctccc tgccgctgag tatatcaaca aagtaaacat tagtgtttac ctccgtgtgt
    11701 aatttgctcg aaaatagcac taaatcgccg caacggcaga atgcaatgca aaatcaggaa
    11761 ggaagattcc agaatttctt tgcgctaatt tgcaaaactg aataataacc tcaagaaacg
    11821 atcggcatca tagcggcaaa aggtaagaaa aaggtggcag gatgtttacg aaggcgttat
    11881 cggttgtctt attaacgtgt gcgctgttct caggacaact catggcaggg cacaaaggac
    11941 atgaatttgt gtgggtaaag aatgtggatc atcagctgcg tcatgaagcg gacagcgatg
    12001 aattgcgtgc tgtggcggaa gagtcggcgg aaggtttgcg cgagcatttt tactggcaaa
    12061 aatcgcgcaa accagaagcg ggacaacgtt gagtcgtttg atgccggatg cggcgtaacg
    12121 ccttacccgg caggcaatat catgcagatt caataagttg tgagtaacca gaaaactggc
    12181 gttttaccct tttcgcttcg gcaatgtttt cagcagctca tccggactta cggatgccac
    12241 tacgcttccc ggtaacggca ttttcagtat gtgatttttg attttgccaa tgacgtgcat
    12301 ctcgcatggg cggcaatcaa actttagcgt taatacttca tcgccgttaa ccagttgcat
    12361 tggcgtagcc ttccagcttt tgatattgcc tttcgcctgt ttcgggcaca gattaaaggc
    12421 aaagcgcaga caatgcttgg tgatcatcac cgggacttcg cccttctctt catgtgcctc
    12481 atacgccgcg tcaatcagct gcacaccata gcgatgataa aattcacgcg ctttctggtt
    12541 gtaaacgttc gcgaggaaac tcagatgtgt ttgcggataa accggcgcag gatcagcaac
    12601 cggtttacgg ctgccgcgct ggtaactggc aagacgcgca gcatccagca tgtcggcagc
    12661 ttcacggcgg aactggttta acagactgtt tggtacaaac agcgcccccg gcaaattaat
    12721 ttgcacatcg cgggcgtaat agatggtttg ccccagtttt gccagaccat ccttcagatt
    12781 gttcattgct ttttcggcgt tattggcttc gtcgaactgc ccatccagcg tatgcgttat
    12841 gctgaccccc tcttcactgg tgagagtcag aatcaattgc tcctgccaac cgcccagttc
    12901 aatgtctacc gccacccgac gttcgctgga ggtttttgtc agtgcctgct gccagttatg
    12961 atcaagatta cggtttagtg ggtgatgcgg gcgaattttg tgcaaatctg ctggcatttc
    13021 attgggccag acgcggtact gattttctcc ggttttctcg accgtattgg cacgaaaacc
    13081 gacgacttca cgtttaatca tcacgttcag gccatcacca tttgccagtg gctcggtgac
    13141 ggcaacatcg agatgatctt tcgccacttt cactacttcg cctaccggca ggccgataaa
    13201 tttcggcgaa tcgaacgcgc caatatcgcc tttacgggca ttcacaaaat aatctgtgct
    13261 accacggtgg aaagtctttt ccgtcgatgg aacaaagaaa tgttcagtac gacctgatga
    13321 agcgcgcgcc agatcgccac gttcttcaat aatggcatca agcatctggc gataatgggc
    13381 ggtgatattc ttcacgtagc tcatatcttt gtaacgcccc tcaatcttga aggagcgtac
    13441 accagcatca atcagcgcgc cgaggttggc agtctgatcg ttatctttca tcgacagcag
    13501 atgtttttca taggaaacca cccgcccctg atcgtctttc aatgtgtatg gcaaacggca
    13561 cgcctgcgag caatcgccac ggttggcact acgccctgtt tgcgcatgag aaatgtagca
    13621 ctgacccgaa taggccacgc acagtgcccc atgaataaag aattcaatgg tcgcgtccgt
    13681 agcctggtga atcgcgcgga tctgatcaag attcagctct cgcgccagca caatctgcgt
    13741 gaagccaaca tcagagagga acttcgcttt ttctactgta cgaatgtcgc actgcgtact
    13801 ggcgtgcagt tcaatcggcg gaatatcaag ttccagaatc cccatatcct gaacaatcag
    13861 cgcatcgaca ccggtctggt agaggtcagt aatcagccgt tgcgcgggtt ccagctcatc
    13921 atcatgcaaa atggtgttaa gcgtgacgaa aatttttgca ccataacgat gggcaaacgg
    13981 caccagctcg gcaatatctt tcaggctatt actggcatta tgacgggcac caaaacctgg
    14041 gccgccgata taaacagcat cggcaccgtg caaaatagct tcgcgggcaa tggcggcatc
    14101 gcgtgccggg cttaacagtt caagtcgatg agaagatacg gtcacattct tatccagtag
    14161 ctaaaaaatg gcggctattt tagccacaag cgcgacgttg cgaaatagtt ttcccgctta
    14221 actgcgcggg taatggatga gggaatgaaa atgtacgatt tgctccccac cattacgata
    14281 gatatgcgtg acgtctgcag caaatcgagc gccttcccca caattaaggc tctgccattc
    14341 tccatcaacg cacagatcaa gttgtccatc aatgacgacg acatgttcga ttaccccctt
    14401 ctcatggggc gtcgattcgc ttattgcacc gggtgccatc tgaatcgaga agtgttcaaa
    14461 gcagagttgt ggatcataag gaaacagcga agtgatcacc attgcctgct gttgtggatc
    14521 ataaactgaa ggcgtagcgg actgtggcgg agaaataaat gtcgaaaatg gcacgttcaa
    14581 cccagtggca attttccata atgtcgcgac cgtcgggctg gactcattac gctcaatctg
    14641 tcctaacatt gctttagaaa cgcccgtcgc ttctgccagt cgcgaaagac tccagccgcg
    14701 ctgctgacgt agttgtttaa gtgtggtgga taaaaagcga gcaagatttt ccatgcgact
    14761 cctccggcaa aacggaagtt tatcacttgt gcgttataac ggacaaatgc tacggtgcct
    14821 gtacgctata acgcacgagg tgactatgcg tctgttttct gttcctccac ccacgctact
    14881 ggcggggttt ctggcggtat taattggcta cgccagttca gcggcaataa tctggcaagc
    14941 agcgattgtc gccggagcca ccactgcaca aatctctggc tggatgacgg cgctggggct
    15001 ggcaatgggc gtcagtacgc tgactctgac attatggtat cgcgtacctg ttctcaccgc
    15061 atggtcaacg cctggcgcag ctttgttggt caccggattg cagggactaa cacttaacga
    15121 agccatcggc gtttttattg tcactaacgc gttaatagtc ctctgcggca taacgggact
    15181 ctttgctcgt ctgatgcgca ttattccgca ctcgcttgcg gcggcaatgc ttgccgggat
    15241 tttattacgc tttggtttac aggcgtttgc cagtctggac ggtcaattta cgttgtgtgg
    15301 aagtatgttg ctggtatggc tggcaaccaa ggccgttgcg ccgcgctatg cggtaattgc
    15361 cgcgatgatt attgggctcg tgatcgttat cgcgcaaggt gacgttgtca caactgatgt
    15421 tgtctttaaa cccgttctcc cctcttatat tacccctgat ttttcgtttg ctcacagcct
    15481 gagcgttgca ctcccccttt ttctggtgac gatggcatcg caaaacgcac cgggtatcgc
    15541 agcaatgaaa gcagccggat attcagctcc tgtttcgcca ttaattgtat ttactggatt
    15601 gctggcactg gttttttccc ctttcggcgt ttattccgtc ggtattgcgg caatcaccgc
    15661 ggctatttgc caaagcccgg aagcgcatcc ggataaagat caacgttggc tggcagctgc
    15721 cgttgcaggc attttctatt tgctcgcagg tctgtttggt agtgccatta ccgggatgat
    15781 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt taagtaccat
    15841 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgcgacgcgg cggtggtggc
    15901 atttctggta acggcaagtg gattgacgct ggtcgggatt ggttctgcgt tttggggatt
    15961 aattgccgga ggtgtttgtt acgtggtgtt gaatttaatc gctgacagaa accgataata
    16021 aaaagcgcca tctaacgatg gcgcgtctgg aactactttt tgttaacgtc aaacatggtg
    16081 gcatcggtcg ccatgtcatc aataacttgt ttaatatttt cgaaagccat tggcgtactt
    16141 tcgttattca ggtctttacc ttcgccctga cgaacgactt tgataaccgg tttattagtc
    16201 gctgcatcaa tcagctcacc ttcaaaatac aagcgagtat ccatggttcg gtggcctgta
    16261 gccatttgcg tccccgcaac cactaatgca acaggaacca cttcatagaa ttgcagccct
    16321 tctttgctgg tatctacacc ggtaatggcc ccacggaaaa tcagactacg cggccccgcg
    16381 gtggtaacca gtggtttacg ctgcgctatc gcttctttca tttcggtgtt ggtatagttc
    16441 aaaattttat ccagaacttt ctgccctacc tgggtcgaag gttttggcac cggataataa
    16501 gtgattgggt tccagacgat gctgtcatat ttgctttgat caaaactcgg gtctacccaa
    16561 cgtaaaaccg gtttacctgt agccgaggtt gtttctttta aatcagagta attgtttaaa
    16621 aaaccagaat atttatctgg ctgggtgatt ttagaggcac aaccagacag agccaacaag
    16681 ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt cctgagatga
    16741 ttatattact ttttaagtta tagcaaaaag cgccgcttcg tgttgtggca aaaaaagtgt
    16801 tggtgaaaat aaattttttt agcccctttt cagacgacat agtcaatgcc gcaaaagggg
    16861 ctaagtcaat catcagtagt taaatttcag taagttaagc tgctaaaaaa cttcgactac
    16921 tgtgattaat ttcacatccc ttcgataaaa aatatgctta ccgaaaagaa ggacagtaaa
    16981 ccatctcagc gtggggaatt ttcacgcgct ttgttcttac cgggtcattt tttacgtgcc
    17041 agcatcgtgg cgaaacgcag tttaatacga ttaccgttgg cgtcggtgcg gtgcagctcg
    17101 ccgacgtctt cattgtattt caccatctcc cagccttcgt aatatcgacg taattctccc
    17161 tctttgaagg caaacgggaa gccgacggta catggataat cagcggtatc catcgccgcc
    17221 acaatcaggt tgtaaccgcc aggtttagtg caacgttgca tattggcaat cagccctggg
    17281 atggttttag cctcaaggaa catcagcacc acagtcgaaa gaataaaatc gtactgtcca
    17341 gcaaatgtga ggttattcag atcaacgacc cgagtgtgta aattatccag attttcaata
    17401 gatttaatgc gctcgacgtt ggcgatactc atggcatttt tatcccatgc gtcaacatca
    17461 taaccattgg ctgccaggta aagactgtta cgaccattgc cacagcccag atccagcgtt
    17521 ttacccggtt taaccacttt caccgcttcc agtacttcag agtgtgtgcg ggttaattca
    17581 tatttatcag taaaatagtt ttcgtcacga atgatcattc tttgtcctct gctttcatta
    17641 aaacggcgcg ctcggttctg actaacaatt ttccctgcat cagaagcgca aaagtacgga
    17701 tgagcagtat tgcaataata aaattggtaa agataaacag cggcaccgcc agcgtatgga
    17761 aaaatccatt atcgctgcca ctccccagat gcaaaccggt ggttgccagt gcagatacgc
    17821 cgaacgagaa actccagaat gaagcattaa atggctggga gagataccat ggcatcagac
    17881 gtagcataaa cagcagttgc agtagtccat aaccgaaaag cattttcgcc agcgtgtcac
    17941 cctcgccgcc gttgacgctc agccaggcac tacaggccac cagcgcagga gcgagttgaa
    18001 tgccgagaga tgtccgcagt gccgtgggta attctcccga actgcgcaga cgctgcaaga
    18061 tcaccggttc aaggcttagc catgagaaaa cgcctgcgcc taaaaacacc agaccggcgt
    18121 cggtgtagcc caacgcacca caggccattg cgctgataaa gttgttggca actgtcggca
    18181 gatacagtcc aggcgtggta gcttcttcag ggtgagatcc gcgccataat cccgccgttt
    18241 gccaggcggc ataagccaac tgaaccacga caccaaaact gaacaggcac accgccagtg
    18301 ggcgaaacca cggaacaaaa ccaatcgcca ccagcatcgt cgttgccgga aacaaactca
    18361 caaaactgct cagcactgga tggcgaactt ccgccagcac gctatgcgga aagcgtatga
    18421 gtcgggtaat aaatgcgcta gtcaataatc cccagatgat catcgccaga atcaccagcc
    18481 catcccctaa ccagtggctg acctgccaaa cctgactggc atagcgccag gcaaatccca
    18541 tccctatcgt ccccaacaca ataccaaagt agcctgccgg caaattgagc actttatcac
    18601 tctgcatctt cttactcatt ttgtttaatt tataaagtat atttgaaatg cattttatgg
    18661 aattttctcg agcatagcca gagccgcaga atttgctacg gttatagtaa gtcacctgtg
    18721 ggaaaacgct atgcgcaaat atcgtctaag tgaagaacag cgagccttta gttatcagga
    18781 agatggcact aataaaaatg tgttattacg gcagattatc gccattagcg attttaacga
    18841 tgtgatagca ggaaccgctg gcggctggat cgatcgcgaa acggtgttag cacaggaagg
    18901 aaattgctgg atttacgacc agaatgctat tgcgttcggc ggaacggtga tttccggtaa
    18961 tacgcgcatc accggcatca gcgtgctgtg gggagaggtt tacgcaacgg ataacgtctg
    19021 gatcgataat agtgaaatca gccagggtgc acatataagt gacagtgtca ccattcatga
    19081 ctcattagtt tgcgggcaat gtagaatatt tggtcacgcc ttaattgacc aacattctat
    19141 gatcgtcgcc gcacaaggct taacgcccga ccatcaactc ctgctacaaa tttatgatcg
    19201 cgcaagggtt agcgcctcgc gtattgttca ccaggcacaa atttatggcg atgccgtcat
    19261 ccggtatgct tttattgaac atcgcgccga agtttttgat tttgccagta ttgaaggtaa
    19321 tgaagagaac aacgtctggc tatgcgattg cgctaaagtc tacggtcacg cgcaggtgaa
    19381 agctggcata gaagaagatg ccattccgac gattcattac agttcgcagg tggcggaata
    19441 tgccattgtg gaaggtgatt gtgtgttaaa acatcatgtc ttgattggcg gaaatgccgt
    19501 ggtacgtggt ggaccgattc tgctcgacga gcacgttgta atacaaggtg agagccgtat
    19561 tacaggtgcg gtgataattg aaaaccatgt ggagttaacc gaccacgccg tcgttgaagc
    19621 ctttgatggc gacacggttc atgtccgtgg tccaaaagtc attaacggcg aggagcgcat
    19681 tacgcgaacg ccgctggcag ggttattatg aatcgataat acgcgcgtat aagttcacat
    19741 catcataggc atcattcagg aactcagcct gtttcaggca accttcaagg ataaaactat
    19801 tgcgcaaagc gacctggttg ctttgcggat tgtccacccg acatttgatc acgaagcgtc
    19861 taagttcacc agactgggcg taatgatgaa tcaatgcctg cagcgcctga gaaatgatcc
    19921 cctgcccctg atgagattca tccagccagt agccgatttc agcggtttta ttcagtggtt
    19981 caatacgatt aaacgagata acgccgataa gttcatcttc tttgaaaatc atgaacattt
    20041 tggcatagcc gcgttgatgc aacatcacat taccctgcac cgtttttcgc gtgtcctctt
    20101 cactctgaac gaactgcggc cagtttagcg actgctgtaa ccaggtttta tttttacaga
    20161 ttaactgata aagaggagtg acgtgatttt ctgcaacagc atgtaattca agtgattcgc
    20221 ttacttttat cgtttcagtc atcttatctc cagtcaatcc atgcagttgt taaaaaagcc
    20281 gcctggctgg cggcttagga aagtttacac ctctttgacg ctctcctgct gcaggatttt
    20341 gagcatctca tcttttaact gtagcttttg ttttttcatg cggaccactt ccgcattgta
    20401 ccctcgaccg tcggaacctt cctttctggc aatttcatga tcaagtttat tgtgtttatc
    20461 gaacaaggac ataaagcgag gattttcgtt tttcagacgg gatattaaat ctcggtattc
    20521 tggaaacata cgcactccct gttagtggct gacaaggtat atctgaaata cacaccatca
    20581 gattactcat ttaacttagg gaaaaatgcg agcgggatcg tgtttaacca gacggcacct
    20641 cccgaagaag gtgccgaaga aaaaacaatt aactcatcac gcggtcgtca acatactgac
    20701 gtttatcggg cgctggcggg aaatactgat acagccatgt ttcactgata gcgtcgccct
    20761 gacaacgcag atacatccgc atatcgaccg gatcagtgga gtccgacgtc ggataccagt
    20821 caaactgaat acgataacca tcaatgggtt caatatagag aatttcgatt tgcttcgctt
    20881 ccccactgga aagcgtaatc accggctcaa tgccttttgg cgcggcagct ttcagatcac
    20941 caccaacgaa atcgacggca aaacgacgcg cccatttttc gggatagtgt tcacctggcg
    21001 cccaaccttc cgggaaaccg cccatgccgg tacgcgtcgc cataacgcgc gctaatgggc
    21061 aatgaacagg cggttgcgca ctccagtaca gacgatactg gaatgcaaac tcatcacccg
    21121 cttttacagc tttttctggc tgccagaagc agacaatgtt atccagcgtt tcgcccgttg
    21181 ttgggatttc catcaggccg atggtgccct taccccactt gttacgcggt tccacccaca
    21241 gacttgggcg tttgttatac cagcccataa tgtcctgata atgggagaag tcacgatcca
    21301 gttgcaataa accaaaccct ttcgggttgt tgtcggtgta agcattgaac tgcaattttt
    21361 gcggattatt cagcggacgg caaatccact cgccgttgcc ccgccacatg gacagacgat
    21421 cagagtcatg aatttgcgga tgaatggtgt cgcacatccg acgttcatta gtaccgcagc
    21481 tgaacatgct ggtcatcggc gcaatgccca gctgtttaat gtctttgcgc gcatacaggt
    21541 gattttccac atccataatc acctgacttt tctcacaatg gatagtgaac ttataggcac
    21601 cagtaatgct ggcgctatcg agcaacgcat aaacggtaaa agtagttgcc cccggtttta
    21661 ccgtatcaaa ccagaaggcg gtaaagtcgg ggaactcttc tttactgtcg gtgtaagtgt
    21721 cgatcgccag gccgcgggcc gacaaaccgt attgatatgt atcatcaacg gcgcggaaat
    21781 aactcgctcc gaggaatgat actacatcac ggcgcgccag ttcgggggct ttaaacacgc
    21841 gaaaaccggc aaagccgaga tcgctttgcc cttctaattg tttggtatca acacctgcat
    21901 cgttgtattt gaacaactcc gggcgaaagt gaatttcacg cgccagatgt gttgctggat
    21961 ctacagaaaa catacgaacg cggcgacgga atcccattcc catatggaag aactgagcgt
    22021 ccagttgacg gttctcaacg ttatgccaga gcgatttttc ggcgtcgtat tgaatactgt
    22081 tataagcctg cggcgtcatt gtcgccagcg tgtcaggtaa cggacgaggc gcaccacgcc
    22141 acgctgtttg cgctaaatca tgcgccattg actgtagaat ggagaagtca aaacgctggg
    22201 tttgcccatc ggcaatatca gaatctgccg cgaatgccgc ctgagaaaaa agagaagcaa
    22261 tgccgctggt accgcacacg gcggccatag ccattgaacc tttaataaat cgtctacgat
    22321 ccatacctga aagtgagtcc ttctggtgtt gtgaatggtc ccgcgctggt cgttttcagc
    22381 ggcaagaaag tgcttattta tgaccgctca ctctagacaa tatttattaa taatccaatt
    22441 gttgaactca gagaatatgc ataaatcggc aattttttta tgtcgatggg atgagcctga
    22501 ttcgtttgta aaatcaggca aatttttgca aaggcggatg gtcagaattt aaagcaaacc
    22561 acagtgacgt ttactgcgtt cttccgcttc ctgataaagc tggaactcat ccaaaaccgg
    22621 acaacccaac gcctgctcaa atgcttcgcg actggcatta ccgtggctgc gtgcctgcat
    22681 ttcattaccc agatttgact ggaagatccc cgccgcgcta acgggcaaga aatcttcata
    22741 ggtaatgggt tgcgccacta cccaaccacg ttcaattaag ggttgtggat cgtctccggg
    22801 atgaatcgcc tgacgatgcg tctcacccga aggcgtcaga cggtaccgga accatgccag
    22861 cccttgctga cgcattaaaa actcactgtc aggaaaagtg cggaaggttt cctgtaaatg
    22921 catttggtga gtgagattat cctgcccggt tccagcgtta cgcagaagat catcatacag
    22981 ttgtcgccct ttcggcgtta atgccacgcc acgctgctca atttcaccaa agcgcgcagt
    23041 atgcgtgccc tgtttctgcc ccgcaaacaa caccgtctct tccagtgctt taaagctggt
    23101 ctggcgtagt aaaatcggta cctcgcggcg cggcggccct tcgatcagaa tttttggttc
    23161 aattccgcat tcaggcatca tcgactgcac ccggtcaata tccagcgtac gtggcgtcag
    23221 atggttgata tggcatccag gaaaacagac cacatcagca atcaaccgat gttcgttgtg
    23281 caaggcgcga taggtttctt catctaccgt tgctgactgg tgccagcgaa acgtttccag
    23341 ggcctcctgc acaaactcct gtgcctgtgt ttcgttaaaa ccgccctgct gctcatattc
    23401 ctctaacagt tgtcgacaac gtggggtgaa tatatcgcgc tgacgcagaa tctccgccgc
    23461 tttctggcgc aaaatttcgt tctcgataag ctcaaggcgg agtaaggagg taaaaacgcg
    23521 gaagggatta cgcgccagag aagcatcatc aatgggccga aatgctgtcg aatgtaccgg
    23581 cacccctgcc tgcgagagat cgtaatagct caccgggtac atccccataa tggcaaacat
    23641 ccgccgaaga gtagcaagct cttgtgcagt cccaacgcga atcgccccat gacgttcaac
    23701 attcagtcgc gccagctcgt ctgcatttac cattttttcg tgcagttgag gattgttttc
    23761 cagcacagcc agattcacat cagctaccag ttccagcagc gtgccgtact gcggaacttc
    23821 ttgctggtac atggctgaca ttgcctgcga aaactgttcc cgaatctcat ccgccgtgat
    23881 gctgttcgcc atagtgtctg cctccagtgg tcagtaatct ggagtgtagg taaccgcatt
    23941 cactctggcg ggaagaattt acaaactgtg atctcgccgc gaaaacatca atattatcta
    24001 ttttgctgta acataattac tttaattgtt aataatattt tgcaatcaag ttatcataat
    24061 caaacaactt cacttgtcag cgacaccgct tcgtttttaa catcgcttat ggaaaaaaat
    24121 agtctgttta gtcagcgcat ccgtttgcgc caccttcata cattcgtagc tgtcgcacaa
    24181 caaggaactt tggggcgcgc ggctgaaacc cttaatttga gtcaacctgc gctctctaag
    24241 acattgaatg aactggagca gctgacgggc gctcgcttgt ttgagcgtgg tcgtcagggg
    24301 gcgcaactta ccttacccgg cgaacaattt ttaacgcatg cagtcagagt tcttgacgcc
    24361 atcaacactg ctggacagtc gcttaatcgt aaagaaggtc ctaataatga tgtcgtcagg
    24421 gttggtgcac tacctactgc ggcactgggg atattacctt cggttatagg tcagtttcat
    24481 cagcaacaaa aagagacgac cttgcaagtt gcgacaatga gtaaccctat gattctggcg
    24541 ggtttgaaaa ccggggaaat cgatatcggc attggtcgga tgtcagatcc tgaactgatg
    24601 accgggctta attacgaact gctgtttctt gaatcgctga agctggttgt ccgccctaat
    24661 cacccgctac ttcaggagaa cgtaacgcta agccgggtgc tggaatggcc ggtcgttgta
    24721 tcaccagaag gcactgcgcc acgccagcat tcagatgcat tagtacagag ccagggatgt
    24781 aaaattcctt cgggttgtat cgaaacgctg tctgcttcgc tatctcgtca acttacggtt
    24841 gaatacgact acgtgtggtt tgtcccttct ggcgcggtaa aagacgacct gcgtcatgcc
    24901 acgctggtgg ccctgcctgt tccgggacat ggtgcaggcg aaccgattgg aatactgacc
    24961 cgcgtagatg cgacgttctc ttctggttgc cagttgatga ttaacgctat tcgaaaatca
    25021 atgccgttct gaaaggtgaa gggatctgtc gatccctcct tgaacatttt cacaccgtag
    25081 cgaaactaac ttgttcaccc gctccgcgag gttctgccga cacagaatgt ttgttcagac
    25141 ggaatacgtc caccgcctca gttaatcgtg ccgcctgttc ttcaagggac accgccgctg
    25201 ctgaggcctc ttctaccaga gaagcattct gttgcgtcac cttatccatt tcagaaatcg
    25261 cctggctaac ctgagttata cctctacttt gttcatccga ggcggcggcg atttcctgca
    25321 tgatatgtgt gacactcgcg acggcatcaa caatagtgct catggttttc cctgccgttg
    25381 ccacctcatc cgaccccagg tcaattaacc tgactgattc actgatcaag ccttcaatct
    25441 ctttcgccgc ctgagcgctg cggcttgcga gtgtccgtac ttcgctggcg acaacggcaa
    25501 atccacgccc ttgctcaccc gctcgcgcgg cttcaacggc agcattcagt gccagaatat
    25561 tcgtctggaa agcaatactg ttgatgacgg cggtgatctc agaaattttc ttcgaactca
    25621 tagagatagc gcccatcgtt tttactacac cggaaaccgt ttgcccgcca tcgctggctt
    25681 taatagaagc ctcttgcgcc agtttgctgg catgatgcgc gttatccgca ttctgtttca
    25741 ccgtcgcagt gagttgctcc atgctggcgg cagtttgttc gatagccgcc gcttgttctt
    25801 cggtgcgaga tgaaaggtcc gcattgccag cggaaatttc gctggtgcca cgataaatct
    25861 cttccgcgcc ctgtcgaaca gtccctactg tcatccccag tgaatgctgc atttgctgta
    25921 aatgacgact taagcgaccg atttcattac gacccgccgg ttcatcattc atcgtcagat
    25981 cgccactggc gattttttca atccgttgtg cggcatgttg cagtggacga atgacgatct
    26041 gacgtagcac cataaatgtt atcagcgtca tgaccagggc aagcacaaac gcgccaatca
    26101 tgaacatccc acccacgcgg gtgcgctgat gggccagttc cgccagttgg ttggctctgg
    26161 tgctacgtat cttaacggct ttgttcaaaa tatcggtata agcattatcc agcgttcgga
    26221 tctgctcact ttcatgattg ataatcgctt caaacatgcc atttttggca tatttcaaca
    26281 taggttgcat acccgtgata taagcctgga agcgttgatt taattcagtg tcgagggctt
    26341 catcagcagg tgttttcacc ggccgattct gataagcacg ataaccttgc tgcgactgtt
    26401 taatctccga ttcggcttgc gcaatatttc gcttcattgc ttccatttcc gcaatacgac
    26461 tcgcagcccc ggcctgaatc atattgatcc gggctgaacg caaaaaatct gaactgttcg
    26521 ctaacccggt ccgaatctca atctccgcag taacatcacg ctgatcgcga tcggcctgcc
    26581 agaggaaata gccggcaagg gatgaactta atgcgaatag aactaagata ccgccaagaa
    26641 tgcaggcaaa taacggaacc agcctgatgt gatgcaaaaa acctaatcgc tgtgagggag
    26701 ttgtattcat taccgttgtc tctcgtccag gtttactcct gatagaaagt catcggcatt
    26761 tgacggtaat tacttacgac caaaaacctc tttgcgtcac atttttacaa caattattac
    26821 agaagagatt aatgccattg gaatgaagag agtaacgaat ggctaaaccg aatgacgaaa
    26881 aattctggaa aacagcacgc aatttcgtga agtgattatt ttcgtcccta aggaaacctc
    26941 ataggatgcc tgcgggtgcc tgagactttc tgttttcagg ctaaccggga atcagaaaga
    27001 cgaaggcccc gaccgatatt tctatcaacc gaggccaacg aatgaacata gcaattcaga
    27061 tcttagcctc ttacccgcca tccggcaagg agaaaggcta tgaaacgcaa ccctctggtg
    27121 gtgtgtctgc tcattatctg cattacgatt ctgacattca cactcctgac ccgacaaacg
    27181 ctctacgaac tgcggttccg ggacggtgat aaggaggttg ctgcgctcat ggcctgcacg
    27241 tccaggtaag ggcaagcgcg gggattttcc ccgtgcatct gactagcaga ttcattttct
    27301 catggcaccc cttttacact tcagttagtg catgtttttt tcgaggcact aatagaacca
    27361 tcattacaga caaatttgcc gcctgccgta cagtgtgaga tccccccttt cgaaccagaa
    27421 caaggtttgc gcccggcatc ggcataaaag gaaacaagcg ttcccataaa caaaataagt
    27481 gccagttttt tcatataacc tccatggttt atcgaatctt atgttcgatt tgaacgttat
    27541 acgctggttt attttgatac cgttgatgag atcaaagatc gggtgaattg attaaaaata
    27601 agccagatgg acgtatgggc aaggattatg gttttatttg tcacacaaat aaatataata
    27661 ggcgcttccg atgtagaccc gtactcttcg cctgtaccac gggtcggttt tagtacaggc
    27721 gttttcttta aatatccttc aggaacgttt acgcggcatc atgcgtagaa gtgtgttgtc
    27781 cttccagaaa tagtggtgtg ccagtgcagc cgcagcgtgc aacccgatga caaaatatcc
    27841 cagattcgcc agcgtttcat gccacgactt taagctatct acccgttcga aattggcctc
    27901 tgaagcgtaa ggcatcgtca aaccaaacgc aaaccacggg ttgccccggt tatacatcat
    27961 caccaaacca atcactggca gcgcaataaa caggagataa atcaccaaat gtcccaaatg
    28021 cgccagtccc gtcatcatcg gtttcggctt aggtataatc ggcggggttg ggtatttcag
    28081 tcttaacaaa agacgaacca ccatcagcac gagaattgag atgccacagg aaacatgaat
    28141 catgttgatg agcggccgat cgctacgtgg gaagaaaccc cgaaactcca ttgcgcaata
    28201 cgctgcgata accagcaaaa agaccagcca gtgaatgctg atttgtaacc ttgagtattt
    28261 attttccata acatttcctg ctttaacata attttccgtt aacataacgg gcttttctca
    28321 aaatttcatt aaatattgtt cacccgtttt cagtacgtta cgtttcactg taagaatgga
    28381 gattgcccat cccgccatcc tggtctaagc ctggaaagga tcaattttca tccgaacgtt
    28441 cctgacagga gaaaaccatg agtaaagttg gtattaacgg ttttggtcgt atcggtcgac
    28501 tggtgttgcg tcgattactt gaagtcaaaa gcaacataga cgttgtcgct attaatgatc
    28561 tcacttctcc aaaaattctc gcctacctgc tgaaacatga ttcaaactac ggaccgttcc
    28621 cctggagcgt tgattttacg gaagattcac ttatcgttaa tgggaaaagt atcgcggttt
    28681 acgccgaaaa agaggctaaa aatattccgt ggaaagcgaa aggtgcagaa atcattgtcg
    28741 aatgtactgg cttttatacc tccgccgaga aatcgcaggc gcatcttgat gctggtgcga
    28801 agaaggtgtt gatttccgct cctgccggtg aaatgaaaac catcgtttat aacgttaatg
    28861 acgacactct ggatggcaac gacaccattg tttccgtggc gtcatgcacc actaactgcc
    28921 ttgcgccgat ggccaaagcc ttgcatgaca gtttcgggat agaagtcggc acgatgacga
    28981 ccattcatgc ctatactggc acccagtcac tggtggatgg cccgcgtggt aaagatttac
    29041 gtgcttcacg cgcagcggca gaaaatatca ttccccacac tacgggcgcg gcaaaagcca
    29101 ttggtctggt gatcccggaa ctgagcggca aactgaaagg tcatgcgcaa cgcgtgccgg
    29161 tgaaaacagg ttcggtcact gaactggtat cgattctcgg aaaaaaagtg actgccgaag
    29221 aggtgaataa cgcacttaaa caagcaacca ccaataacga gtcatttggt tataccgatg
    29281 aagaaatagt ctcttccgat atcattggca gccatttcgg ttcggtgttt gatgccacgc
    29341 aaacggaaat taccgccgtg ggcgatttac aactggtgaa aacggtcgcc tggtacgata
    29401 acgaatatgg cttcgtcacg cagcttattc gcaccctcga aaaattcgct aaactctgac
    29461 gcgcacaggc ggaggaaaaa acctccgcct ctttcactca ttaagactgt aaataaacca
    29521 cctgggtctg cagatattca tgcagaccat gtttaccatc tgcgccgcca ataccggatt
    29581 tacgccatcc ggcgtgaaag ccttgcatag cttcgaagtt ttcacggttg atgtaagttt
    29641 caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg gtatagattg
    29701 atgaggtcag gccgtaatca ctgtcattag ccattgagat agcatcttcc agcgtgtcaa
    29761 atgcgacaac tggcagcacc gggccaaagg tttcctcatg cataatcgac atttcctggc
    29821 gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc gctttgccac
    29881 ccaatgccac tctcgcccca tcttctactg cgcgcgctac tttttgctca accctttcca
    29941 gcgccgcggc gttaatcaac ggtcccatcg caatgtcgtt gcgttcagcg gggttaccaa
    30001 attgaaccgc ctgcaacgct tcacccagcc gattgacgaa ctgatcataa atgcctttct
    30061 gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg cgtgaatcaa
    30121 cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc ggtgctttac
    30181 cccccagttc caggcacact ttggtgatgt ttttcgccgc agtcgccata atcttttcac
    30241 ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc gccagttctt
    30301 gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga aggcctattt
    30361 catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg ggtttaatga
    30421 caatggtatt accagtcaaa agagcgggag ccattttgcg ggcaatgagg aagaacggga
    30481 agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaga agaatatttt
    30541 ctcctggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac tccgccatgt
    30601 aatcgatata gtcggcagta aaagccactt cgacttcagc cagctgctgg atcttgcccc
    30661 cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatcccg gcggagattt
    30721 tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt tgtgcacgtt
    30781 ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtatg cgggaaatga
    30841 cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag gtaacaaact
    30901 gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactcc tgtgatttat
    30961 atgtttgttt tcggtgaata cattgttaat taacatcaat catcagagag acggcatcgt
    31021 gaaaggacaa ttctgtgaag ggcttcataa aaacaagtgt aaaaggaata aaaggcagaa
    31081 ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg caatttttaa
    31141 cgtaacgagc gactttccat cgcctcgatg agcaccgcat cgtgtttcag cgtttgccat
    31201 gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc gcggggacca
    31261 tagccatcgc tatcatcgcg taaacgcggc agctcgccat tgagtagtga gagataacgc
    31321 tcaaccggcc ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct
    31381 aactgaggaa cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccggtc
    31441 atacggcgga acgttgccat cgtgcgccgc tgcatggtgg ggtcctgtac aacgatagcc
    31501 gtatgaactc gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg
    31561 cagtttgttg actggtcttc aatccagatt ttttcatgcg gaatatgcca gaactgatga
    31621 gcgatatccg ccaggatggt cgcttctgct ctgccagtgg tgcggatagt attgtagtgc
    31681 ggatgctgtg cgatggcgct atacaaaaaa gtggtcgagt gaccgatacc accactaatc
    31741 agtaaaggag tttgctgatc gcgagcaatc ttacatgccg catcgatagt cggcataacc
    31801 gcatttcctg caagtatgac gcaatcagcc tgatacggca cctcaccgga gaaatcatcc
    31861 tgcgccagcc actgtccgat aacatttatg gcatctatag ttgccgtcga aagcgtcgga
    31921 aacggggtaa tgttcatggt tcctccttat cgttcccttc agaatactgc gtgagataat
    31981 agtgagaagc ggagcactct gaaaggagga agtcgtatgt ccttaaccgc cactccgcaa
    32041 gcctgacata tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa
    32101 ttgtattttt ctgaattcct gaatttttgc ccttaccgtt ttagaattaa taaaaaaccc
    32161 ggcgctaaat gcttaccggg ttttagattt atcaggcaaa tagcagggtt aaccgctaat
    32221 ctgctccatc gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg
    32281 agcgaagtaa ctaacgcgca gctcttctat catccaacgg atctctttca cgtcttcatc
    32341 ctcacgacgt gcaggcggga gtttgttgaa ccattgctgc cacgcctgct ggactttttc
    32401 gactttcagc atctgtgcac ggtcgcgatg tggatcaacc gccagttttt ccagtcgttt
    32461 ttcaattgcc tgcaaatatc gcagcgtgtc gcccagtcgt ttgaagccgt tagcagtaac
    32521 aaaaccgcga tacaccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat
    32581 ggtcatatcc acccgccctt tcagacgttt gttgatattg aacactgccg taagaatttg
    32641 ctcgacctgc ttcgcaatat ctaccaccgt gtcgttcagt tcggcacgca ctttctcatg
    32701 cagcgcagca aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc
    32761 cacaccgcat gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta
    32821 cagtcccagc ttggctttgt ttggtaactt ctcatgcaag tacttgatcg gcgatggaat
    32881 attcagcaac agtaaacggc gaaggccgtt ccacattgcc tgcttttgct cgagcgggtt
    32941 atcaaacagt ttgatcgcca cgctgtcgcg ttcatccacc agtgccggcc acgccttcac
    33001 tttgtagtta ccgcgcttct gctcgtagct ttccggcagt tgtccaaagc tccagatatg
    33061 cagtccgctc tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc
    33121 tttcagcgca tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc
    33181 atccaccacg cggaaggtga ttttcaggtg atcgggcact tgatcccagt gccagtcttc
    33241 gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg
    33301 taactccagc ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggcg cgggcacaaa
    33361 attacggcgt accggtttcg gcaacgattt aatcagagca atcaccagtt cgcggcgcag
    33421 accagggatc tgccactcaa acccactctc ctcaacctgg ttaagtaacg gcagcggaat
    33481 atgtacggtc acgccgtcag catctgcccc cggctcaaac tgatagctca aacgcagctt
    33541 gagattgccc tgatgccaga agttcgggta atccagcttg ctgatttttt ccgccccttc
    33601 tttgatcaac atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt
    33661 ccaccagctg tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa
    33721 ctcaaacaac gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttcaagctc
    33781 ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc
    33841 accttccacc agcgcgtggc gaataaagag ttcacgacat aacgccggat cgatctggct
    33901 gtagttgacc ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc
    33961 catcaccgcg ccctgcgccc gttcccagtg cggttcgcta taggtgcgtt taatcaaatg
    34021 ctgagcaacg ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg
    34081 gctggtttct accagttccg ccaccattac ccatttcggc ggctttttga ataaaccgga
    34141 accagggaag atggagaaac gcgcgttacg tgcgccagta tattcctgtt tatcggcatc
    34201 tttcatcccg atatgggaaa gcaaacccgt cagcaacgca atgtgaattt ctcggtattc
    34261 cgccggttcg ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta
    34321 gatatcctgc cattcgcgca cgcgcagata gttgagataa tcggtacgac acaggcgacg
    34381 gaaagcgttt gaagagagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa
    34441 cgcgaggaaa tcggattctt tgtcgtggaa gcggcgatgt ttttcgtccg atgcctgctg
    34501 tttatccatc ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc
    34561 ctcacgcaca cagccgtgtt tctgcgcttc aagcaccata cgcgcaagtc gaggatcgac
    34621 cggcagttgc gaaagctgac gcccgagcgg cgtcagttta taggcgctgg cctgttcatc
    34681 cgtggtgatc gcgcccaatt cttcgagcag acgcacgcca tcctggatat tgcgtttatc
    34741 cggtgcttct acaaacggga acgcggcgat atcgcccaac cccagcgcgg tcatctgcaa
    34801 aataaccgag gccaggttag tacgcagaat ttccgggtcg gtaaactccg ggcgcgagag
    34861 gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgac cacaacggcc
    34921 tttacgctga ttggcagacg cctgcgaaat cggctcaatc ggcaaacgct gcactttggt
    34981 gcgatagctg tagcggctga tacgcgctgt accggggtcg ataacgtgtt taatccccgg
    35041 tacggtcagc gacgtttccg cgacgttggt cgccagcaca atgcgccgtc cgctatgcga
    35101 ctggaacacc ctattctgtt cgctgttcga aagccgcgca taaagcggca agatttccgt
    35161 atgtcgtaag ttcagtttgt tcagtgcatc agcggtatcg cgaatttccc gctcgccgct
    35221 cataaagatc agaatgtcgc cggggctttc ctggctcagt tcgtctacgg cgtcaaaaat
    35281 cgcctgcaat tgatcgcgct cggtgtcatc ggcttcttca acaatcgggc gatagcgcac
    35341 ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga
    35401 aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg
    35461 cagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctacgtt cgtgcgcttc
    35521 atcgataatg atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag
    35581 gataccgtcg gtcatcagct tgaccatcgt gttatcgctt acgtgatcgc tgaaacgcac
    35641 tttgtaaccg atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac
    35701 cgttcgtgcc gccaggcgac gcggctgggt atgaccgatc agccctttaa taccgcgccc
    35761 caactccata cagattttcg gtagctgagt cgttttaccg gaaccggttt ccccggagac
    35821 gatcaccacc tggtgatcac gaatcgcttc gagaatgtcc tgctttttct gactaaccgg
    35881 caaattgtcc gggtaagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc
    35941 cgcctggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt
    36001 aaccttcttc acaccatgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga
    36061 atccagccgc tgctgcaaag cagtaaaggt caatttttgt tgttctgtca taacgttaga
    36121 gggcagtagt ctgcctgatt aattttcttt taaatgatag atatagagta ccacatcgcg
    36181 gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg
    36241 tggcaggatt gtgaataaag tgtcagcaag caacggggca tcgcccattc aaacatctat
    36301 aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc
    36361 agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg
    36421 aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg
    36481 gtgcgctgcg tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcacttt
    36541 ccgatgagct gattgccgag ctgaaagccc atgacgttat cgttattgcg gcaccgatgt
    36601 ataacttcaa catctcgact cagttgaaaa actattttga cctggttgca cgcgcaggcg
    36661 ttactttccg ctataccgag aatggtccgg aaggtctggt aacgggtaaa aaagccatcg
    36721 ttattaccag ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc
    36781 tgtccacgtt cctcggcttt atcggcatta ccgatgtgaa atttgtcttc gccgaaggga
    36841 tcgcatacgg tccggaaatg gcagcgaaag cacagtctga cgcaaaagca gccatcgaca
    36901 gcattgttgc tgcataaccg gacacggtct tgatgtggac tgtagatatt cagtccacat
    36961 ctcaatccac ttacctgttt tcccacaatc tcagcatcgc tttgtgttcg tctgtcagca
    37021 caatctgcgg gcgtctggct cgaatatagc taatcgcttc attaacggtt ttacagtgtt
    37081 cgtaacataa caaccatgcc gccaccacca gcgcactgcg cgataatccc aatgcacaat
    37141 ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga
    37201 gttccccctc ttccggaacc accagatcca acatcggtac acagaaatag agtcgatctt
    37261 ttgtctctcg tcctcgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa
    37321 tatgtcgtgg aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc
    37381 gacgacaaaa ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt
    37441 aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaaacccac
    37501 cgtacccacg gccaataatg agtagcgata atactggcca acataaccag actgaccacc
    37561 ataactgaac catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat
    37621 atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa cgacggtcga
    37681 cgggtaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
    37741 gatgctgcca ggttgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt
    37801 tacgccaact cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
    37861 tatgcagcga aggagactgg ttatagggca gatcaaacag ttcaagttgc gaaaatagcc
    37921 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
    37981 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
    38041 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
    38101 caatagtcca ggggagaaaa gggatcgccg tttcccagcc gaatacctga ctggggatat
    38161 catggctgtt aaagtcctga accgcggtga actgattaag agatccatag gtgaagaaga
    38221 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cggcgttcag
    38281 ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat ccgttgtgtg
    38341 catttatcaa atccggcatc acgcacgagt gaatccattt ccccttgcga acgcacgcgc
    38401 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaattag ctccagttgt
    38461 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga
    38521 cctgcgaggg agttctttac ctgctcattt tcgggaaaaa gctcatacag tccagagaca
    38581 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcaccc
    38641 tgttcaaaac gcacctgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
    38701 acatttaact cgctgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
    38761 gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
    38821 ttggcgtgga gatcggcaac ggcttgttta atcagtattt gtaaatgggt tttgcgctgg
    38881 cgaataccgc gccagccaac actgttcagg tagattttgt cgactaaacg cccgaatgcg
    38941 ttactacctt gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagccg
    39001 gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat cgccttacgc
    39061 ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
    39121 catcgatccg ctgatggtcc agtgcagtct tcatgctgat aatcaaattt ttgcgatttg
    39181 ttagcatata agcgactaat aaagctttgc attttttcaa atgccagcgc cctgttctct
    39241 tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttgag agggctacgt
    39301 aaacgctgat aaaaatcgat ctgcggttgg cgatgcacca catagtcatc gcctgatatc
    39361 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
    39421 tagagatcta gcaagatatt gacggcaatc gcccttgtga tcagctgatc attattgaaa
    39481 ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga attaataaaa
    39541 aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
    39601 ttaaccttaa aggccggaga agccagcagc agcccgcgaa ttgcaggcgc ataatcatga
    39661 acccatgtgg caaccagtac tgcgccgacg ctctgcgcga tcactaccac ctcttccagc
    39721 ccgacctggc tgtcgctggc agcaaaacgg acaaactcat cgacatcccg cactgaacgc
    39781 gcaagagatg ggctataacc acggggcccc gaagtttgtc catgccctcg ggcatcccat
    39841 gcataaaaag cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa
    39901 tgttcatgac cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc
    39961 cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
    40021 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
    40081 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctggt
    40141 aatagttttc cgctatcttt aacacccaca tctcgtttga cggctgacat cactacatcg
    40201 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
    40261 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgatgac gccccccacc
    40321 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
    40381 ttcccccata aatactgtgc aatatcgttt gactctgtta acgccaaaag aaataacacc
    40441 agtaacgccc cgccctgggt atttgtggtt ggcaacatta ataaccaggc ggcatgactc
    40501 aaggcaaaaa cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg
    40561 gagggatcac ccacaaaaac tcgccaggtg gttaatatca gaaacccagc caaaggtata
    40621 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
    40681 caatataacc aacgcggaaa atgtacggat ataagcgtac aatattcttt caatgccaga
    40741 aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat caccagtgaa
    40801 aaacaaatga cgatacccca ccaggtacga atacgtaatg taacttcatc cccttttctc
    40861 tccggtaacc gccatacccg aaaacggttt atcagcgttg ctaaaattaa caacgcgaaa
    40921 agtgttgcca gagatttttc cagcattatc tttcagcact catcaggagc acactccgac
    40981 aacgattaat cgcagtccag ataagcagaa ttgaagcaat gccccataaa agattattcc
    41041 attgcatcca ctgtggataa atggcaacgg ccagccccca caacccaaat attaacgcac
    41101 gatcgctttt gccgaatgga ccggcataac tgcgaatacc gttaatcgtc tgcgcgagta
    41161 aaccgcaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt gatgcgttac
    41221 tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca tcgccagttt
    41281 cattcaaaat cgcccccaga cgtgtttgct gattgcactc acgcgccaac atgccatcca
    41341 gcgcattgag tgccatacgg ataaaaagca cgatgggcaa tagcaaaaag aggatgggtt
    41401 gtgcgaccaa catcaacagc aatccggtaa taagagaaag cgccagtgca gcaagagtga
    41461 tgtgattcgc tgtaacatgg tgcttataaa gccaaaacat cgtcggcctt aacagcgact
    41521 gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc ggtttgcgct
    41581 atggtgcatc aattcccctg acggagatat cgcaacacga cgcttcatta aaaattcaga
    41641 atatatcaag gtattgggga ctcactgtca ggaatgatta tcccaataaa tcaagtggtt
    41701 aaaaattatt acgctgttat tcaatacaac tcatcaagtt ttctcatcgc ttgttcccgt
    41761 attgcgcgcg tcttaccgtt aagcgccagc tggcgaacta aatccagttc agggttaaca
    41821 agctctaacg ccagcaaagg atcacgtttc agcctcgcct ttaatacatc aactggaaaa
    41881 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtactgca
    41941 ggcggtgtgt ggcggttgtt aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
    42001 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
    42061 tcggggtcta gtgcataata aacacgtaac gcttcgttaa cctgcgcacg ggaactgttt
    42121 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
    42181 ttgagaacaa cagcgggtaa attatcccaa ctaattaatg cactctctaa aaagtcccag
    42241 tcgtccgaac gtatcagaga ggtggcattt aatagcatct ggtcatcaac ttcaccgtag
    42301 ctgataagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttg
    42361 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
    42421 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
    42481 cgttgtaaag cagcgtcaga gtgaattatc tgcatatccc gacagcaaaa gtaggcaggg
    42541 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa cgctaaaaat
    42601 aacgcaccta ttagatcatc gtcattctcg aatttttggt ataaatatac tgctattttg
    42661 tcactctctt gtggcgtcat ccgcaacaat tgaccatggt gtttcatctc tgccagcttt
    42721 aacgctaagc ttgtgacaac atcgtttgat gaatcgttcg ccaactgcat tgcctgattg
    42781 taggtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
    42841 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
    42901 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
    42961 agattcaacg gaccgacacc atcaggtaga tgactcattc gcgtcattat ttcgacagag
    43021 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
    43081 aactccagaa tctcattgtc aggcatatat ctcgccatac ttgcaatcgt ttgtccatca
    43141 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
    43201 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
    43261 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
    43321 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
    43381 tctttcagag catcttcatt atttattaga ccccaataat atgcatcagg atttattatc
    43441 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
    43501 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatat cccacgcgca
    43561 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
    43621 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
    43681 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
    43741 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
    43801 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
    43861 ctgttgatag cttgttcaac ttgttgttta tcttcagcgg tagccgcaga aaaaaatggt
    43921 tcaatatcaa ctgcatttgc aacacatcga agttccgcct gtactggaag actcagcata
    43981 aacaggggaa gtaaacctat ccagctgcaa atcctgggct taaacatatg cactccatgc
    44041 cattaaaaaa gaaacaaaag ggaaatgcat ctttcccttt tgtatattcg ttattctcgc
    44101 gaaataccat ccaacgtaga caacacttcc tcagaaagat gcaatttttc agcagccata
    44161 ttctcccgta aatgcgctac agaagacgtc cctgggatca gcaaaatatt cggtgaacgt
    44221 tgtaacagcc acgccagcgc cacctgcatt ggtgttgcac ccaggctcgc agcaacatcg
    44281 gaaagcgtgg aagattgcag cggcgtaaag cccccgagcg ggaagaacgg cacataggca
    44341 atgccttcgc gggccaaagc atcaatcatt gcgtcatcag cacgatgagc aatgttgtat
    44401 tcgttttgca cacagacgat ttcggtaatc ttacgcgcct ctgcaacctg cgtcggtgtg
    44461 acgttgctca ggccaatatg ttttaccagg ccttgttgtt gcatctctgc cagcacggtc
    44521 aggctggctt caatcgatcc ttccgccggg ccatgaccat cccccatcat cacgcgcagg
    44581 ttaaccacat ccagtacgtc caggccgaga ttacgtagat tatcgtgtac cgctttttgc
    44641 agctctgccg gagaaaaggc gggcaaccag gaagcgtctt ctccacgccg cgcaccaatt
    44701 ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagcg cttcgcggat aatctgattg
    44761 gtgacgtgcg gaccataaaa atcgctggta tcaatatgat tgacgcccaa tgccagcgcc
    44821 tcgcgcagca cggtaatagc gacatggcga tctctggggg ggccgaaaac cccaggacct
    44881 gccagttgca tcgcgccata accaagacgg ttaacggatt ttgtaccgag agtaaatgta
    44941 ttgctgctca tcgcaccctg ccctgtaata aaacggatta agaaaaatca gtatagaaat
    45001 gaatagagat aaataatgcg agggcgtaaa caagattact ggatgggttg ctctgttatc
    45061 ctgaaagaca ccaaaacaaa acgcgtcaag cgccgcatct ggcaataaaa aaaccgcaac
    45121 ctgtatcagc gttgcggttt aggtatcacc tgctaaaaat tagaacgttc agcatcagga
    45181 cgttactgtt tcggtcgcag attctcatca aataccaggc gctgccggcc aggttcaact
    45241 tcccgcaatg gctcgacttg atgcaacgtc tgcttacagc gtgtcaccag cacctgatac
    45301 tcatgcgtgc cctgcttttt ccatgccagt tcctgctcac taagttcacg aatcgctttg
    45361 gccgggctgc cgacaatcag gtaattagca ggcatttctg ctttggcttt cacaaatgcg
    45421 gatgcgctaa caatgctgtt ctcgccaatc accgcaccgt ccatcactac cgcgttcatt
    45481 cccactaatg cattgcggcg gataatgcag ccgtgaagga tagcgctatg accaatatgt
    45541 ccatcttctt ccacgacagt atcctgctcg ggaaaaccgt gcataacgca attatcctga
    45601 atgttcgcac catctttcac cacgatacga ccaaaatcgc cacgcaagct ggcatttggc
    45661 ccaacgtaaa cgcccttgcc gagaataaca tcgccgatca ataccgctgt cggatggaca
    45721 aagctctctt ctggcacaac cggagtcaga ccatctatct ggtaaattgg cataacgcct
    45781 cctgttcaat atttaagccg ccaaaacgtt gaaaatacag gcttcccggc gcaggcagtt
    45841 caccgaccga ggtttcacct ttttcactaa cgaacgctaa agcagcaggc gctacgcgct
    45901 gataaatgtt gatacacagc tgacgtgccg tatgccctgc ccagtgtgcc ggaagcaact
    45961 cctccggcaa caatgggtct ttaaggacga cacggcgata aaaatggatc agtaaaagct
    46021 gaatatgaaa tgcacgctcc ggggttaact cgtctgccgt ctcttttaac agcggcacca
    46081 gcgggcggaa tgactgaata aaggtttcgt acatggcatt ttgttcagtt aaatgccagc
    46141 actcttccac tctggcacgc agtgctgcac gagaaagcgc cagtggtatt tgcgcttcaa
    46201 aacaaatcac gttatccgcc acacccgctt catgcaaaag tgtctgtaca tccgccagtt
    46261 tttgcgacgg cgatgccatc aggctgggcg ccagtgcacc aaaaccttgc cagatcaact
    46321 gctttttgac atcagccagc gtcgctttat ctaaaccttc cgagagcaac aggagccatt
    46381 taccatccca tgcaggttgc tctgcgcgat aaattttact ttctgcccgt cgcgtcaggc
    46441 gcaagccttt atcactgagg ctatagaaac tgcgtcgccc gatgcgggaa acatccagcc
    46501 agccttcttt attaagacga aacaaagcgg tgcgcacgaa acgctcacca aatcccagcc
    46561 cttccagcaa agcagccaga ctacccagcc agatttcacc accacgatgg gaaagcgaat
    46621 caccatacag agaggagatc aaagatgtgc cactgaccgg aacagcgttt acagcatgtt
    46681 ggataaaagt atcaagttta ctcattcgat tcagtctgtt gttattgttg tgatttatga
    46741 atcatagcac aggtcgtatc gacttctggc cctggcgctt taccccacca gggccagacg
    46801 acacatcagg caccaacaat attacgcaga tcaaacaccc gacacgcctt gccttctgaa
    46861 cgcgggatac tgccacagtt aacgatcatc acatcggtag agatccccac catcgactta
    46921 atccgatggc gtagctggtg gcacacctgg caacgctgct catgcgtcaa tgtcaggctg
    46981 ctttctttca gctctacttt cacagaaagt gaatcaagat gcccccggcg gttcacctcc
    47041 agttggtaat gcggcgataa aaagcggaga tgccttcatg acgatcttct gttgccgcca
    47101 gcaaggtgaa taactgtcgc tcctgggcaa gtccggcttg caaagccact tcctgcgact
    47161 ggcgcagcgc ttgctttgcc gcttgtaagg ccagcggcga gtgacgtgcc attttcgacg
    47221 ccagctgtaa ggcgtattcg agggttaaat cgctggggaa gacgtcgcta accagcccgg
    47281 cctgctgtgc ttgctgagcg gtgatacttt ctccgctcag caccattttg ctggctaacg
    47341 atttaccgac actacggatt aaacgttgcg ttccgcctgc gccaggcatg atgccgagag
    47401 tgatttccgg caacccaaaa cgcgcgttct ctccggcaac caccacatcg cacaacaatg
    47461 ccagttcgca acccgcacca agcgcgtaac cgttgacggc tgcgatgagt ggtttgttaa
    47521 aggcctgcaa tcgcgcccat agctgcggac gtgtatcgtt taaggtggcc gcgagatctt
    47581 tttccgccat ttcgttgaga tcggccccgg cggcaaaaaa gcgtgcatta ccggtaatca
    47641 cacagaccga aatactgcta tcggtagccg cagcttccag ttcatttacc agttgcgtca
    47701 gcagggcatt atttagcgca ttacgtgcgg cgggacggtt aagggtcagt aacaatactt
    47761 gttgctgacg gctgacgatc agttcgctca tgccatcccc ttcgcgtcaa agtcaaccac
    47821 cacatcgctg gtcagcggca gtgcctggca actcaacaca tagcctgcag ccagttcatc
    47881 cggttccaga ctgtaattgg tttccatcgc cactttgcca cgcagcactt tgcatttgca
    47941 ggtcgcacag acgccgcctt tgcaggcata gggcagatca gccccctggc gcaatgccgc
    48001 atcgagaatg ctttcatcgt cggcattaag cacgatttcc cgatcccgcc catcctgacg
    48061 tacagtcact ttttgtccgt cactttgcac gttaacgcta cgtttgacgc gcgtgccagg
    48121 cgtattaaac cgctccagat gaatcgcttt ttctggcatt cccagtgctt ttaaggcagc
    48181 ttccgcgtca tccatcatcg ccgccggacc acaaataaac gcctcatcat aaagacgaaa
    48241 attaatgagc gaggccccaa gtgactgtaa tttttcacca tcaatacgcc cgtgaagcag
    48301 atcgctgtcg agggtttcct gactgaaaat gcacaacaac tgtaaacgct gaggatattt
    48361 gtctttcagg tctgccagtg cctggcgaaa catcatgctc tggctggtac ggttaccgta
    48421 gatcagggtg aactgacttt caggctcggt ttgtaaagtg gtggcgataa tagccagcat
    48481 tggcgtaata cctgatcctg ctgcaatagc cagatagcgc ccctggcgtt cggcctgtgg
    48541 ctgatagccg aaatgcccct gcggcaccat gacctccagc gtcatcccct ggcggatgtg
    48601 ttcgcgggca taacgggaga aacgtccgcc ttcaatagct ttcaccgcca cactaatttc
    48661 gccaggcagg tagctgcggc aaatggagta acaacggcgt aattcttcac catcaaggct
    48721 ggcttttaag gtcaaatgtt gaccggggcg aaagcgatac gcctcctgca aaggctgggg
    48781 caccgcaaag gtaatggtca ccgcatcacg ggtttccggc tccacttttg ccaccgttaa
    48841 ggaatgaaac gttgtcatgg catcctcaaa tacatttgaa ataatcgaaa ggttcgcggc
    48901 aactatcgca gcgatacaat gctttgcagg ccgtggaacc aaattcactg ataagtgtgg
    48961 tatggacgct ggcgcaacgc gggcaacgta cttctggcgg caaatgggca tggcaactat
    49021 gtccggcggg cgggctgatg ccatactgtc gcagacgctc acgggcatcg ggggtcatcc
    49081 agtcggtggt ccatgccggg tcgagttgca gcacaacctg aacgggggta aacccgtggg
    49141 ttgtcattgc ctcacgtatc gcgccaatca gatgttccgt tgccggacaa ccggaatatg
    49201 tcggcgtaaa gccgatcacc catccttctc ccatctgtgt cacattacgc accatgccta
    49261 aatcggtaat ggtcagcacc gggatctccg ggtccgggat ctggctgagt agcgcccata
    49321 tctcatgcac ctgcggcggt gcaatggtgg ccagacgttg catacccatc tcctctgtta
    49381 ccactgctga ccgggcaaga cacgctggag atactgcatt tctgccagca ttggcccaag
    49441 gtgttcggta tgcagtcctt ttttaccgcc ggtgcgatac gcctgctctt gcggaacgtt
    49501 caatgtggct tcgttgatcc cggcaaaaac ttcggcttcc cacgctgcgc gtaaagtgcg
    49561 tggatcaacc gcaataccct cttcctcagt gcaatatcaa tctcgtcggc atcgaacagt
    49621 tcggcagtaa aacgccacaa cttgtcgatg gcctgctgca tcttttgccc tgatacgtca
    49681 gtaccattgc ccagtcgctc cagccagcca cgactaaaac gcaggtgata acgcgcttct
    49741 ttaattgctt tggcagaaat cgctgccagt tgcggatcac ggctttccat cagacgggta
    49801 aagagcgcca catgccatgc atcgatgaaa tactggcgtg caatggtgtc ggcaaaattg
    49861 ccgtttggtt gttcaaccaa caataagttg ctgaactggc gctcgtctcg ggtgaaggcc
    49921 agggtatctt catcgccttc tcccgctaat tcagcagcat acgataagaa gttgcgtgcc
    49981 tggcctaata aatcgaggcc aatgtttgcc agtgcgagat cgatttccag ttccggtgcg
    50041 tgaccgcacc attcacccaa ccgctgggag agcaccaggc agttatcgcc caggcgcaag
    50101 gtgtaagccg ttaactgatt catttccgac ctcacatgtg ctcaatgcca tcagggatgg
    50161 tgtaaaacgt tggatggcga tagaccttgc tttcagccgg atcaaaaaat tcaccgcgtt
    50221 cttccggttg cgaggcaaca atttcactcg ccttcaccac ccaaattgaa catccttcgc
    50281 tacgacgggt ataagcatca cgggcatttt ccagtgccat ccgctcatcg gcagcatgta
    50341 aactgccaac atggcggtgt gataagccct gtttgccacg cacgaaaact tcgtataacg
    50401 gccagtaaac attactcatt ttgaatctcc ttatgcgacc ttacaggcat gttgtttttg
    50461 tgcatgggcc agcgctgctt cccgtaccca ggtaccttct tcccaggatt tacgtttagc
    50521 gtcgagccgc tcctgattac aaattccgcg accgttaatt acttcgttaa actcctgcca
    50581 gtcgatctca ccaaagcggt agtgaccgct ttcagtatca aaatgcagat ccgggtcagg
    50641 aacggtcatg ccgagcattt caacctgtgg aacggtgtta tccacgaaac gctggcggag
    50701 ttcgtcattg gtgaaacgct tgattttcca ggcgagactt ctggcgctgt ttggcgagtt
    50761 atcatcgttt ggcccgaaca tcattaaggc tggccaccag aaacggttaa tggcgtcttg
    50821 caacatctgc ttttgagctt cgctgccctg cgccagtgcc atacaggctt caaacccctg
    50881 acgctggtga aaactctctt ctttgcagat tttcaccatt gctctggcat acgggccata
    50941 agaagtgcgg cataacgcca cctgattgac gatcgctgcg ccatccacca gccagccgat
    51001 aacaccgata tcagcccaac tcaatgtcgg ataattaaag atggaggaat atttcatccg
    51061 tccgtcgagc attttttggt agatgtcttc tctggcgcag cccagtgttt cggcggcgct
    51121 atagagatac agaccatgac cggcttcatc ctgcactttg gctaacagaa tggctttacg
    51181 ccgcaaggtt ggtgcgcgag tgatccagtt accctcaggc aacataccaa caatctcgga
    51241 gtgcgcatgc tgcccaatct ggcggatcag tgtctttcgg taagcatcgg gcatccagtc
    51301 ctgcggctcg atagccgttt cctgggctat ccgttgctca aagcgttctt cttgggtcac
    51361 gtcatcacct ttacgattcc agttttcgtg aattaattaa aaacacgaat cacgttgttt
    51421 tatgaaagtt acacaatggt taactaaagt cacaaatttt gtatgtttga actgtgacgg
    51481 atttcgcact ctattttgat aattcattat ttatcagtgc attaatgttt ttagtagttg
    51541 atatacagat cacgcagtta acatttcatt aaaacacgcc ttgcttttta tgattcgtga
    51601 tttaactatt agcaacagaa atgtgaaata tttggagagt agcgatgcag cagttagcca
    51661 gtttcttatc cggtacctgg cagtctggcc ggggccgtag tcgtttgatt caccacgcta
    51721 ttagcggcga ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg gctgcccgcc
    51781 tgtttgccat tgaaaaaggt gcccccgccc tccgcgctat gacctttatc gaacgtgcgg
    51841 cgatgcttaa agcggtcgct aaacatctgc tgagtgaaaa agagcgtttc tatgctcttt
    51901 ctgcgcaaac gggcgcaacg cgggcagaca gttgggttga tattgaaggt ggcattggga
    51961 cgttatttac ttacgccagc ctcggtagcc gggagctgcc tgacgatacg ctgtggccgg
    52021 aagatgaatt gatcccctta tcgaaagaag gtggatttgc cgcgcgccat gtactgacat
    52081 cgaaatcagg cgtggcggtg catattaacg cctttaactt cccctgctgg ggaatgctgg
    52141 aaaagctggc tccaacgtgg ctgggcggaa tgccagccat catcaaacca gccaccgcga
    52201 cggcccaact gactcaggcg atggtgaaat caattgtcga tagtggtctt gttcccgaag
    52261 gcgcaattag tctgatctgc ggtagtgcgg gtgatctgtt ggatcatctg gacagccagg
    52321 atgtggtgac tttcacgggg tcagcggcga ccggacagat gctgcgagtt cagccaaata
    52381 tcgttgccaa atctatcccc ttcactatgg aagcggattc cctgaattgc tgcgtactgg
    52441 gcgaagatat caccccggat caaccggagt ttgcgctgtt tattcgtgaa gttgtgcgtg
    52501 agatgaccac aaaagccggg caaaaatgta cggcaatccg gcggattatt gtgccgcagg
    52561 cattggttaa tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc gtggtcggtg
    52621 atcctgcaca ggaaggtgtg aaaatgggcg cactggtaaa tgctgaacag cgtgccgatg
    52681 tgcaggaaaa agtgaacaca ttgctggctg caggatgcga gattcgcctc ggtggtcagg
    52741 cggatttatc tgcggcgggt gccttcttcc cgccaacctt attgtactgt ccgcagccgg
    52801 atgaaacacc ggcggtacat gcaacagaag cctttggccc tgtcgcaacg ctgatgcctg
    52861 cacaaaacca gcaacatgct ctgcaactgg cttgtgcagg tggcggtagt cttgcgggaa
    52921 cgctggtgac ggctgatccg caaattgcgc gtcagtttat tgccgacgcg gcgcgtacgc
    52981 atgggcgaat tcagatcctc aatgaagagt cggcaaaaga atccaccggg catggctccc
    53041 cactgccaca actggtacat ggtgggcctg gtcgcgcagg aggcggtgaa gaattaggtg
    53101 ggttacgagc ggtgaaacat tacatgcagc gaactgctgt tcagggtagt ccgacgatgc
    53161 ttgccgctat cagtaaacag tgggtgcgtg gtgcgaaagt cgaagaagat cgtattcatc
    53221 cgttccgcaa atattttgag gagctgcaac caggcgacag cctgctgact ccccgccgca
    53281 caatgacaga ggccgatatt gttaactttg cttgcctcag cggcgatcat ttctatgcac
    53341 atatggataa gattgccgct gccgaatcta tttttggcga gcgagtagta catgggtatt
    53401 ttgtgctttc tgcggcagcg ggtctgtttg tcgatgccgg agttggtccg gtcattgcta
    53461 actatgggct ggaaaacttg cgttttatcg agccagtaaa accaggcgat accatccagg
    53521 tgcgtcttac ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa gaaaaaccaa
    53581 caggtgtggt ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg gtggcgctgt
    53641 attcaattct gacgctggtg gccaggcagc acggtgattt tgtcgattaa tcggtgaatg
    53701 aagggcaacg accaatagtt gcccttttat ttcaccaaat tttgtgacgt tgtcacatta
    53761 tgcatgatgt atatatctat tttcagggca cccactgtat gaaaagctgg cacacctgcc
    53821 aaacaacctg gcaggtgcag gcaatcccct ttacatcagt actgataatg tgaacctgac
    53881 taaaccgccc acagagcgcg gttgctaaca agaacacaac atctgacgag gttaataatg
    53941 ggaagcccct ctctgtattc tgcccgtaaa acagccctgg cgttggcagt cgccttaagt
    54001 ttcgcctggc aagcaccggt atttgcccac gggggcgaag cgcatatggt gccaatggat
    54061 aaaacgctta aagaatttgg cgccgatgtg cagtgggacg actacgccca gatctttacc
    54121 ctgattaaag atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc aattgttaat
    54181 ggtcagcctc tggcactgca agtaccggta gtgatgaaag acaataaagc ctgggtttct
    54241 gacaccttta ttaacgatgt tttccagtcc gggctggatc aaacctttca ggtagaaaag
    54301 cgccctcacc cacttaatgc gctaactgcg gacgaaatta aacaggccgt tgaaattgtt
    54361 aaagcttccg ctgacttcaa acccaatacc cgttttactg agatctccct gctaccgcca
    54421 gataaagaag ctgtctgggc gtttgcgctg gaaaacaaat cggttgacca gccacgcaaa
    54481 gccgacgtca ttatgctcga cggcaaacat atcatcgaag cggtagtgga tctgcaaaac
    54541 aacaaactgc tctcctggca acccattaaa gatgcccacg gtatggtgtt gctggatgat
    54601 ttcgccagtg tgcagaacat tattaataac agtgaagaat ttaccgctgc cgtgaagaag
    54661 cgcggtatta ctgatgcgaa gaaagtgatt accacgccgc tgaccgtagg ttatttcgat
    54721 ggtaaagatg gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta tcttgatgtc
    54781 ggtgatggca actactgggc acatcccatc gaaaacctgg tggcggtcgt tgatttagaa
    54841 cagaaaaaaa tcgttaagat tgaagaaggt ccggtagttc cggtgccaat gaccgcacgt
    54901 ccatttgatg gccgtgaccg cgttgctccg gcagttaaac ctatgcaaat catcgagcct
    54961 gaaggtaaaa attacaccat tactggcgat atgattcact ggcgtaactg ggattttcac
    55021 ctcagcatga actctcgcgt cgggccgatg atctccaccg tgacttataa cgacaatggc
    55081 accaaacgca aagtcatgta cgaaggttct ctcggcggca tgattgtgcc ttacggtgat
    55141 cctgatattg gctggtactt taaagcgtat ctggactctg gtgactacgg tatgggcacg
    55201 ctaacctcgc caattgcacg tggcaaagat gcaccctcta acgcagtgct cctgaatgaa
    55261 accatcgccg actacactgg cgtaccgatg gagatccctc gtgctatcgc ggtatttgaa
    55321 cgttatgccg ggcctgagta taagcatcag gaaatgggcc agcccaacgt cagtaccgaa
    55381 cgccgggagt tggtggtgcg ctggatcagt acagtgggta actatgacta catttttgac
    55441 tggatcttcc atgaaaacgg cactattggc attgatgccg gtgctacggg catcgaagcg
    55501 gtgaaaggtg ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga cacgcgctac
    55561 ggcacgctta tcgatcacaa tatcgtgggt actacgcacc aacatattta taatttccgc
    55621 ctcgatctgg atgtagatgg cgaaaataac agcctggtgg cgatggaccc ggtggtaaaa
    55681 ccgaatacta ccggtggccc acgcaccagt accatgcaag ttaatcagta caacattggc
    55741 aatgaacagg atgccgcaca gaagtttgat ccgggcacga ttcgtttgtt gagtaacccg
    55801 aacaaagaga accgcatggg caatccggtt tcctatcaaa ttattcctta tgcaggtggt
    55861 actcacccgg tagcaaaagg tgcccagttc gccccggacg agtggatcta tcatcgttta
    55921 agctttatgg acaagcagct ttgggtaacg cgttaccatc ctggcgagcg tttcccggaa
    55981 ggcaaatacc cgaaccgttc cactcatgac accggtcttg gacaatacag taaggataac
    56041 gagtcgctgg acaacaccga cgccgttgtg tggatgacca ccggcaccac gcatgtggcc
    56101 cgcgccgaag agtggccgat tatgccgacc gaatgggtac atactctgct taaaccatgg
    56161 aacttctttg acgagacgcc aacgctaggg gcgctgaaga aagataagtg attgtttcag
    56221 actaaaaaaa cggcaccagg tgccgttttt tacttacgcg cgaacaagat taataccgta
    56281 cacacaccga cttggtttca caccaaccgt ccaaccagtc cgggccaaaa tcacggcctg
    56341 ttcctgactg cttcatccca ccaaacggta agttagcgtc aattaaggta tggctgttta
    56401 cccacaccgt accagcctgt aagcgatcgc tatattccag agcctgggag agattttgcg
    56461 tccagacgct ggcagttaag ccatattccg tgtcgtttgc cagttgtaac gcctcttctc
    56521 catctgctac tcgcaccagg ttaaccaccg gaccaaacac ctcttcacga gttaagcgca
    56581 atttagcatc gggattaacc accagcgttg gcgcaacata atacccctct ccggctggtc
    56641 cactcgaccc gcgaatcagt tctgcttgct gtgcctgcgc atcgtcaagg aatgaacgca
    56701 ctttgtcgca gtgcgcacga gaaaccaaag ggttaatctg tgcaacaggt gacatccccg
    56761 gtcccacttg caacgatttt accgcctgct caaatccact aaccagcgtg tcaaacaacg
    56821 gcgcttcaat ataaattcgc gaactggcgg cgcatacttg cccctgattc aggaagcttc
    56881 cggtcatcaa gccttcaata acccattgcg gatcagcatc ttttaataca attgccgggt
    56941 ttttaccgcc cagttccagc gttgcacgcg tcaagcgatc tgcagcagtt ctggcaatac
    57001 ctttgcccgt cgcggttgaa ccggtaaaac tgattttcgc gacatgagga tgtgacgtca
    57061 gggccgcgcc gcatacagca cctgacccgg tgacgacatt aaaaacgcca tcaggaatac
    57121 ctgcctcgct ggccagttcc gccacgcgca acatcgtcag tggcgtggtt tccgaaggct
    57181 taatcacgat tgaacagcct gcagccagtg ctggcatcac cttccacata ccaatcatca
    57241 atgggaagtt ccacggcaca attcccgcca ctacgccaac cggctcttta cgtgtccagg
    57301 cctgatagcg cgccccctag ggtaagggaa tcgacaagtc cagcgtctta cccgcaattt
    57361 tggtcgttaa cccggcagta taacgcatcc agttcagcgt acagcccact tcaaaagcac
    57421 gggaaatggc aattgacttg ccttgctcca gggtttccag ttgcgccagc tcctcactgt
    57481 gctgctcaac cagatcggca aaacgtagca ggatacgttc acgctctgcg ggtaatcgcc
    57541 cggcccagcg acgcgagaca aaggcccgcc aggcagacat tactgcgtta tctacatccg
    57601 cttcgttggc atcagcagtt gatgcaattt cttgcccggt tgccggatca aagatcgcta
    57661 accgtttttc actttgtgcg gggccaggac gaccatcaat ataaagaccg tgttgacgat
    57721 cgagaaactg ttggacctgg cttaatactg ctacatacgg ctctgtcatc acttttcctt
    57781 attatttacc cagtgtgatt tcagtgtacg cagcgacaag aaaagtcact tttttcgctg
    57841 tgacattcgc tttgtcagct gtgacaagct ccgcaaatcg tgacaataac cgcttatgcc
    57901 tctatgcctg acaagactta tttcctatag tcaggctcat tagcttcgtt tattgcaaca
    57961 caaatgcaac aataaaaata catttcacag agcgacaacg tgccatgaac cccgcagtgg
    58021 ataatgagtt tcagcagtgg ctttcccaaa tcaatcaggt atgcggaaat tttaccggac
    58081 gcctgctgac tgagcgttac acgggtgtac tggaaacgca ttttgccaaa ggactaaagc
    58141 tgagcaccgt gacaaccaac ggggtgaatt tataccgcac ctagcaggag gtaaaaggca
    58201 gtgacgatgc ctggttttac accgtttttc agcttagtgg tcaggcgata atggagcagg
    58261 atgagcgtca ggtgcagatt agcgcaggcg atattacgtt actcgatgcc tcacgaccct
    58321 gttcgcttta ctggcaggag tcttctaaac agatttcatt acttttgcca cgcactctgc
    58381 tggaacaata ttttccccat caaaaacctg tctgcgcaga gagactggct gctgacttac
    58441 ctatggtgca actcagtcat cgcctgttgc aggagagcat gaataacccg gcactttctg
    58501 aaacagaaag tgaagctgcg ttacaggcga tggtgtgtct gctgcgcccg gtacttcatc
    58561 agcgggaatc tgttcaacct cgtcgtgaac gtcagtttca aaaagtggtt acgttgatag
    58621 acgataatat tcgcgaagag atattacgcc cggagtggat agccgcagag acgggtatgt
    58681 cagtacgtag tttgtatcga atgtttgccg ataaaggttt ggtggtcgcg caatatattc
    58741 gtaaccgtcg tctcgatttt tgtgcagatg caattcgcca tgccgcagat gatgaaaaac
    58801 tggcaggcat tggctttcat tggggatttt ccgaccagag tcatttttca acggtgttta
    58861 agcaacgctt tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattcgcg
    58921 ttgcatgtac agcaacgcga attagttaaa acagatattt gactatgcat ttttcaaggc
    58981 gaaaggcaat ttgacaggag tttttttaat attcaccttg tttcaggaaa atagatcaat
    59041 cgccttctcc tgtagcgtta tcccgatagt gcggttttgc tattaaaatt ttcgcaccca
    59101 tttaccgttt atcccttgta catattcccc cggtttagcc cgagctacga gcttttgccc
    59161 ggcgagtttc gcgatatcat ctaccgacac attattttgc tttgccagtt gttggtaact
    59221 cgctttacgt tcggcattaa tgtcggttac taatttctct gtttcagcat ccgttttcag
    59281 cgcaaccaga taaccataaa atgtttcccc gacccgcccc tgagttctgg cttcatccag
    59341 agtcaatgcc attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt
    59401 tttcttcatc atcgcctcag aaaagatcgc tacgagtttc aagcagccct tcgacatctt
    59461 tgtctgcctt gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt
    59521 ccttaggtgc agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca
    59581 acgcagccag taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt
    59641 tcccggcaga cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact
    59701 gcgccacagc gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt
    59761 accatcaacg cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc
    59821 taaattaata tttgtcgatg aacggctaat ttccatatag cgcaaccagt taattgctga
    59881 acccgcagtc atattgtctt tcaccaccgc atctgcggtg tctttatcca gccgcaaggt
    59941 cattggcccg ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca
    60001 taacggcaat gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct
    60061 aaccagttcg ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat
    60121 acgtaattgt ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag
    60181 caatggttct tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc
    60241 tgtcaccaga ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg
    60301 ccaggctcca tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc
    60361 tggcatccag gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc
    60421 aggcacagca gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca
    60481 gtcgggtggc accagcggct ggaatacagt cagtgattgt ttaggccacc aggcgttacc
    60541 gcgcagacga ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg
    60601 taaatcgcct ttaaagagga aatacgtcgg atcgcgccca tcgacgctaa attttaaggt
    60661 tgatggcggt aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc
    60721 gccgctgaag gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa
    60781 tcgcggcttt tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc
    60841 ggtcagcgta atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt
    60901 catcggtgta aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag
    60961 aaaatcattc gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc
    61021 ctgcaaaatg gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa
    61081 aggccagcgg atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa
    61141 cgcgcctggc tcaaaggtca acgttgggtc agacagactt ccacttacct gcgcaggtaa
    61201 acgggcatat aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagctg
    61261 gctgttatcc atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc
    61321 ttgcccctgg gtcagcacac tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg
    61381 ccagttgtcg actttgacac ccagtcggcc actcaaagga aaacctgcat acggccagct
    61441 ccagcgacca tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa
    61501 cggatcgccg ttatcccgtg ccagcacaat caattgcccg ctattttcct gccagtccag
    61561 ctcggcatcc accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgaccact
    61621 tacaggaagt ccatccggca ctagcggcat agtaaattcc cccaccagtt taaccggcgg
    61681 ctgattttca aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg
    61741 gccttgaaat ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc
    61801 gagagagagt tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat
    61861 ccaggtgtta ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg
    61921 ctccgtctgc ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa
    61981 taaccagcgg ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc
    62041 caccagataa cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc
    62101 taatgcaata cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc
    62161 gagcgtcatc agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc
    62221 tttatattta cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa
    62281 tacgggtatt gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt
    62341 tgttgatgaa tttttcaata tcgccatagc tttcaattaa atttgaaatt ttgtaaaata
    62401 tttttagtag cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt
    62461 tatagcacaa aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt
    62521 gagctggaat tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc
    62581 gaagcggtat gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa
    62641 aagcacggcg ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac
    62701 gcggcaaaag aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt
    62761 gctgaacacg ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag
    62821 cgtacccgtg acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa
    62881 acggcaggcg ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt
    62941 tttggtatgc gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt
    63001 gtggagtatg tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc
    63061 ccgctgacac cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat
    63121 ggcgtgatga tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa
    63181 gcgctgaaaa atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat
    63241 ctgttctttg aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct
    63301 gcctgccaca acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc
    63361 agtatttctc agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg
    63421 aacgaactgg tttaatcttg ccgctcccct gcaatccagg ggagctgatt cagataatcc
    63481 ccaatgacct ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa
    63541 ccacaatgaa gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa
    63601 gtaatgacaa aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa
    63661 gcgtaaacgg taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa
    63721 aaatgatgat ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg
    63781 gtgaactgac agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta
    63841 atgaactcga taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg
    63901 cgaaccagtt aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa
    63961 tgaattaata gctgccacag ctcccggcgg caagtgactg ttcactacag cgtttgccgt
    64021 tgggtaatgc acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac
    64081 cgccgatcat tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg
    64141 ctacgtgcgc cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag
    64201 cggcacaccc tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac
    64261 gacaccgcca taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg
    64321 ccccgcattt ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat
    64381 tacgctgatg tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga
    64441 aagattacta ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg
    64501 tcatatgatt actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga
    64561 agttatcgcc atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg
    64621 ggccggaaac ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc
    64681 ggaagcgggt gcaatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc
    64741 gtttacgtca tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga
    64801 actcacaccg tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactctctat
    64861 ttttggcgat cattccgacg tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc
    64921 ggcaaacgtc caggaagcgc aagactttgc tctcatttcg cacatcgcga cgctgaaaag
    64981 ccgcgtgcca tttattcatt tctttgatgg tttccgcacg tcccacgaaa tcaataaaat
    65041 tgtcccgctg gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca
    65101 tcgcgcccgg gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga
    65161 cacttatttc cagtctcgcg aagccaccac cccctggtac aacgcggtct atgaccatgt
    65221 tgaacaggcg atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgaata
    65281 ttacgggcat ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg
    65341 tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg
    65401 tctgtaccgt cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag
    65461 cgtggcggta ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga
    65521 tgtgatgacc gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt
    65581 cattggtggg cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt
    65641 atttgccgag ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga
    65701 tgtgaccaat ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga
    65761 agccttgttt tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa
    65821 gattatcggt aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa
    65881 agcgggcggc ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta
    65941 tctcatttcc caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca
    66001 gatggctgag cgtttaaaac ctggcggcat tttcctgctt aatacgccgt acagcgcaga
    66061 tgaagtgtgg tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg
    66121 cttctatgtg attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa
    66181 taccgtcatg cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct
    66241 cgcagaattg cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga
    66301 gcgcaactgg caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc
    66361 ggtaaatccg cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt
    66421 gaaaaccgta accgctgcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct
    66481 gccgccagac ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga
    66541 agagatcccc atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg
    66601 cccacactca gctatccgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc
    66661 cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca
    66721 ggtggctccg gaagattgca ccggttgtaa cttgtgcgtc gaagtttgcc cggcgaaaga
    66781 ccgtcagaat ccagagatta aagccatcaa tatgatgtcg cgcctggaac atgtcgaaga
    66841 agagaaaatc aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga
    66901 acgtattgat attcgtacat cgcagctgat tacaccgctg tttgaatatt ccggtgcttg
    66961 ctccggttgt ggcgagacgc cgtatattaa attgctgact cagctctatg gcgaccggat
    67021 gttgatcgct aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc
    67081 gtataccacc gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa
    67141 tgccgaattt ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg
    67201 tctgctggat caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga
    67261 cgctacgcca gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt
    67321 tgccgaagca catgaactgc tgcgtgacgc agatgcactg gtagaaaaat caatctggct
    67381 gattggtggc gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag
    67441 tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg
    67501 tcaggcgtcg aaagcgacac cgctgggtgc agtaactaaa tttggcgagc acggcaaacg
    67561 taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca
    67621 gatttctctc ggcgcgcaac tgaaccagac ggtgaaagcg attcaggaag cggaagcgta
    67681 tccggggcca tcgctgatca tcgcttatag cccatgtgaa gagcatggtt acgatctggc
    67741 actcagccat gaccagatgc gccaactcac agctaccggc ttctggccgc tatatcgctt
    67801 cgatccgcgt cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccatc
    67861 agaagcactg gaagaaacgt tacttcatga gcaacgtttc cgtcgtctga attcgcagca
    67921 gccagaagtg gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga
    67981 cttcctggca caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgcactggat
    68041 aaggattatc caattctaaa aaaaaagtcc ggacgactgt tcgggcttgt ctttttatat
    68101 gctggaaaat cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg
    68161 cat
//
LOCUS       DAZUGK010000002        67992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZUGK010000002 DAZUGK010000000
VERSION     DAZUGK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.002"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..751
                     /locus_tag="ACHY5W_000065"
     CDS             155..751
                     /locus_tag="ACHY5W_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445039.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIQPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSQAVTAGHVTTNALYTIAYK"
     gene            838..1563
                     /locus_tag="ACHY5W_000066"
     CDS             838..1563
                     /locus_tag="ACHY5W_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS4445040.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNTQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1605..4205
                     /locus_tag="ACHY5W_000067"
     CDS             1605..4205
                     /locus_tag="ACHY5W_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFS4445041.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTRKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYDKADVRFDGSDQFLDLTVPQAYVLKNYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQNGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     AKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNSGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     DVMIGSMGQGGQAFVRGINDSGELTVRWYENNQTIDCKLHYQFPAQPQTLGSTNTLLL
                     NNLTCQVANH"
     gene            4219..4767
                     /locus_tag="ACHY5W_000068"
     CDS             4219..4767
                     /locus_tag="ACHY5W_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445042.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLLASDCSNGYSKIFTTLTGASV
                     SGKLIVNEASSGAAGVGMGIKRRDAADSAFFTPNNTDKFEWSTDEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4782..5393
                     /locus_tag="ACHY5W_000069"
     CDS             4782..5393
                     /locus_tag="ACHY5W_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445043.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQVLDSSNKATDTVAVGNVYISELAAKSKVQPFKIRFSDCAGLPGKSAQLVLAPRPG
                     TNCAGGQSNTAGFANSISSANGGSSRAAVEVWTTTTPEGSGSQQFSCVQRNVITVDLS
                     NASTTQPYDYDLSARMIIAEGRTVNDVLPGEFLSPTTFTITYQ"
     gene            5414..6004
                     /locus_tag="ACHY5W_000070"
     CDS             5414..6004
                     /locus_tag="ACHY5W_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFS4445044.1"
                     /translation="MLCRHHKLVRFLGLVAALITPFGYAGQDVNLTALIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            6016..7128
                     /locus_tag="ACHY5W_000071"
     CDS             6016..7128
                     /locus_tag="ACHY5W_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445045.1"
                     /translation="MQRKGNNLLIQLCSAILLFFTTSWYALANECYIESNAEGSYDMV
                     INPTPFSIQTRMVDAPEEIAFATWDSHINLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSRGDALLKTTVPGIVYSVELVCKSCGAAEELDLYLPAQSGADNHTDNASAKWA
                     YEYSDDSWYLRFRFFWAPEFKPQTGVSEGTAMPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TIKLKAANKPSDGTLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNAVDYNFAIWSN
                     NLLNFPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            7265..8059
                     /gene="panB"
                     /locus_tag="ACHY5W_000072"
     CDS             7265..8059
                     /gene="panB"
                     /locus_tag="ACHY5W_000072"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS4445046.1"
                     /translation="MKPTTIASLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAR
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8071..8922
                     /gene="panC"
                     /locus_tag="ACHY5W_000073"
     CDS             8071..8922
                     /gene="panC"
                     /locus_tag="ACHY5W_000073"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS4445047.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMSFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9018..9227)
                     /locus_tag="ACHY5W_000074"
     CDS             complement(9018..9227)
                     /locus_tag="ACHY5W_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445048.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9296..10168)
                     /gene="rpnC"
                     /locus_tag="ACHY5W_000075"
     CDS             complement(9296..10168)
                     /gene="rpnC"
                     /locus_tag="ACHY5W_000075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFS4445049.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10442..10822
                     /gene="panD"
                     /locus_tag="ACHY5W_000076"
     CDS             10442..10822
                     /gene="panD"
                     /locus_tag="ACHY5W_000076"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS4445050.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10826..12055)
                     /gene="yadE"
                     /locus_tag="ACHY5W_000077"
     CDS             complement(10826..12055)
                     /gene="yadE"
                     /locus_tag="ACHY5W_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4445051.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12119..12559)
                     /gene="yadI"
                     /locus_tag="ACHY5W_000078"
     CDS             complement(12119..12559)
                     /gene="yadI"
                     /locus_tag="ACHY5W_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4445052.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12664..13434)
                     /gene="yadH"
                     /locus_tag="ACHY5W_000079"
     CDS             complement(12664..13434)
                     /gene="yadH"
                     /locus_tag="ACHY5W_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4445053.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13431..14357)
                     /gene="yadG"
                     /locus_tag="ACHY5W_000080"
     CDS             complement(13431..14357)
                     /gene="yadG"
                     /locus_tag="ACHY5W_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4445054.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14466..15128
                     /gene="can"
                     /locus_tag="ACHY5W_000081"
     CDS             14466..15128
                     /gene="can"
                     /locus_tag="ACHY5W_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFS4445055.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15169..15705)
                     /gene="hpt"
                     /locus_tag="ACHY5W_000082"
     CDS             complement(15169..15705)
                     /gene="hpt"
                     /locus_tag="ACHY5W_000082"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4445056.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15911..18301
                     /gene="gcd"
                     /locus_tag="ACHY5W_000083"
     CDS             15911..18301
                     /gene="gcd"
                     /locus_tag="ACHY5W_000083"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFS4445057.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPIGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18503..20053)
                     /gene="cueO"
                     /locus_tag="ACHY5W_000084"
     CDS             complement(18503..20053)
                     /gene="cueO"
                     /locus_tag="ACHY5W_000084"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFS4445058.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20219..20566
                     /gene="yacC"
                     /locus_tag="ACHY5W_000085"
     CDS             20219..20566
                     /gene="yacC"
                     /locus_tag="ACHY5W_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFS4445059.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20672..21538
                     /gene="speE"
                     /locus_tag="ACHY5W_000086"
     CDS             20672..21538
                     /gene="speE"
                     /locus_tag="ACHY5W_000086"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFS4445060.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21554..22348
                     /gene="speD"
                     /locus_tag="ACHY5W_000087"
     CDS             21554..22348
                     /gene="speD"
                     /locus_tag="ACHY5W_000087"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFS4445061.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22386..22748)
                     /gene="yacL"
                     /locus_tag="ACHY5W_000088"
     CDS             complement(22386..22748)
                     /gene="yacL"
                     /locus_tag="ACHY5W_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFS4445062.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22923..25520)
                     /gene="acnB"
                     /locus_tag="ACHY5W_000089"
     CDS             complement(22923..25520)
                     /gene="acnB"
                     /locus_tag="ACHY5W_000089"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFS4445063.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25875..27728
                     /gene="yacH"
                     /locus_tag="ACHY5W_000090"
     CDS             25875..27728
                     /gene="yacH"
                     /locus_tag="ACHY5W_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFS4445064.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPLSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNSLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTVPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGDHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNQNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKIQINPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27799..29223)
                     /gene="lpdA"
                     /locus_tag="ACHY5W_000091"
     CDS             complement(27799..29223)
                     /gene="lpdA"
                     /locus_tag="ACHY5W_000091"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4445065.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29548..31440)
                     /gene="aceF"
                     /locus_tag="ACHY5W_000092"
     CDS             complement(29548..31440)
                     /gene="aceF"
                     /locus_tag="ACHY5W_000092"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4445066.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPTPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31455..34118)
                     /gene="aceE"
                     /locus_tag="ACHY5W_000093"
     CDS             complement(31455..34118)
                     /gene="aceE"
                     /locus_tag="ACHY5W_000093"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFS4445067.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34279..35043)
                     /gene="pdhR"
                     /locus_tag="ACHY5W_000094"
     CDS             complement(34279..35043)
                     /gene="pdhR"
                     /locus_tag="ACHY5W_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFS4445068.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35584..36957
                     /gene="aroP"
                     /locus_tag="ACHY5W_000095"
     CDS             35584..36957
                     /gene="aroP"
                     /locus_tag="ACHY5W_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFS4445069.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(37000..37854)
                     /gene="ampE"
                     /locus_tag="ACHY5W_000096"
     CDS             complement(37000..37854)
                     /gene="ampE"
                     /locus_tag="ACHY5W_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFS4445070.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37851..38402)
                     /gene="ampD"
                     /locus_tag="ACHY5W_000097"
     CDS             complement(37851..38402)
                     /gene="ampD"
                     /locus_tag="ACHY5W_000097"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFS4445071.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            38490..39383
                     /gene="nadC"
                     /locus_tag="ACHY5W_000098"
     CDS             38490..39383
                     /gene="nadC"
                     /locus_tag="ACHY5W_000098"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFS4445072.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39586..40026
                     /gene="ppdD"
                     /locus_tag="ACHY5W_000099"
     CDS             39586..40026
                     /gene="ppdD"
                     /locus_tag="ACHY5W_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFS4445073.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDILQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAK"
     gene            40036..41421
                     /gene="gspE"
                     /locus_tag="ACHY5W_000100"
     CDS             40036..41421
                     /gene="gspE"
                     /locus_tag="ACHY5W_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFS4445074.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKLVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41411..42613
                     /gene="hofC"
                     /locus_tag="ACHY5W_000101"
     CDS             41411..42613
                     /gene="hofC"
                     /locus_tag="ACHY5W_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFS4445075.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLHIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42648..43691)
                     /gene="guaC"
                     /locus_tag="ACHY5W_000102"
     CDS             complement(42648..43691)
                     /gene="guaC"
                     /locus_tag="ACHY5W_000102"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4445076.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43847..43891)
                     /locus_tag="ACHY5W_000103"
     CDS             complement(43847..43891)
                     /locus_tag="ACHY5W_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445077.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43916..44536
                     /gene="coaE"
                     /locus_tag="ACHY5W_000104"
     CDS             43916..44536
                     /gene="coaE"
                     /locus_tag="ACHY5W_000104"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4445078.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44536..45279
                     /gene="zapD"
                     /locus_tag="ACHY5W_000105"
     CDS             44536..45279
                     /gene="zapD"
                     /locus_tag="ACHY5W_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFS4445079.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLTQQLTVNLPIVDHTGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            45289..45486
                     /gene="yacG"
                     /locus_tag="ACHY5W_000106"
     CDS             45289..45486
                     /gene="yacG"
                     /locus_tag="ACHY5W_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFS4445080.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEDPKQ"
     gene            complement(45693..46091)
                     /gene="mutT"
                     /locus_tag="ACHY5W_000107"
     CDS             complement(45693..46091)
                     /gene="mutT"
                     /locus_tag="ACHY5W_000107"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFS4445081.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGIIPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVR"
     gene            complement(46151..48856)
                     /gene="secA"
                     /locus_tag="ACHY5W_000108"
     CDS             complement(46151..48856)
                     /gene="secA"
                     /locus_tag="ACHY5W_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4445082.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48918..49430)
                     /gene="secM"
                     /locus_tag="ACHY5W_000109"
     CDS             complement(48918..49430)
                     /gene="secM"
                     /locus_tag="ACHY5W_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFS4445083.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(49661..50578)
                     /gene="lpxC"
                     /locus_tag="ACHY5W_000110"
     CDS             complement(49661..50578)
                     /gene="lpxC"
                     /locus_tag="ACHY5W_000110"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFS4445084.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50679..51830)
                     /gene="ftsZ"
                     /locus_tag="ACHY5W_000111"
     CDS             complement(50679..51830)
                     /gene="ftsZ"
                     /locus_tag="ACHY5W_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4445085.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51891..53153)
                     /gene="ftsA"
                     /locus_tag="ACHY5W_000112"
     CDS             complement(51891..53153)
                     /gene="ftsA"
                     /locus_tag="ACHY5W_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4445086.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(53150..53980)
                     /gene="ftsQ"
                     /locus_tag="ACHY5W_000113"
     CDS             complement(53150..53980)
                     /gene="ftsQ"
                     /locus_tag="ACHY5W_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFS4445087.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53982..54902)
                     /gene="ddlB"
                     /locus_tag="ACHY5W_000114"
     CDS             complement(53982..54902)
                     /gene="ddlB"
                     /locus_tag="ACHY5W_000114"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4445088.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54895..56370)
                     /gene="murC"
                     /locus_tag="ACHY5W_000115"
     CDS             complement(54895..56370)
                     /gene="murC"
                     /locus_tag="ACHY5W_000115"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4445089.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56424..57491)
                     /gene="murG"
                     /locus_tag="ACHY5W_000116"
     CDS             complement(56424..57491)
                     /gene="murG"
                     /locus_tag="ACHY5W_000116"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4445090.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57488..58732)
                     /gene="ftsW"
                     /locus_tag="ACHY5W_000117"
     CDS             complement(57488..58732)
                     /gene="ftsW"
                     /locus_tag="ACHY5W_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFS4445091.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58732..60048)
                     /gene="murD"
                     /locus_tag="ACHY5W_000118"
     CDS             complement(58732..60048)
                     /gene="murD"
                     /locus_tag="ACHY5W_000118"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4445092.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVHLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(60051..61133)
                     /gene="mraY"
                     /locus_tag="ACHY5W_000119"
     CDS             complement(60051..61133)
                     /gene="mraY"
                     /locus_tag="ACHY5W_000119"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4445093.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(61127..62485)
                     /gene="murF"
                     /locus_tag="ACHY5W_000120"
     CDS             complement(61127..62485)
                     /gene="murF"
                     /locus_tag="ACHY5W_000120"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4445094.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALANNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62482..63969)
                     /gene="murE"
                     /locus_tag="ACHY5W_000121"
     CDS             complement(62482..63969)
                     /gene="murE"
                     /locus_tag="ACHY5W_000121"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFS4445095.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63956..65722)
                     /gene="ftsI"
                     /locus_tag="ACHY5W_000122"
     CDS             complement(63956..65722)
                     /gene="ftsI"
                     /locus_tag="ACHY5W_000122"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFS4445096.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65738..66103)
                     /gene="ftsL"
                     /locus_tag="ACHY5W_000123"
     CDS             complement(65738..66103)
                     /gene="ftsL"
                     /locus_tag="ACHY5W_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4445097.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(66100..67041)
                     /gene="rsmH"
                     /locus_tag="ACHY5W_000124"
     CDS             complement(66100..67041)
                     /gene="rsmH"
                     /locus_tag="ACHY5W_000124"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4445098.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(67043..67501)
                     /gene="mraZ"
                     /locus_tag="ACHY5W_000125"
     CDS             complement(67043..67501)
                     /gene="mraZ"
                     /locus_tag="ACHY5W_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4445099.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        16432 a        18490 c        16688 g        16382 t
ORIGIN      
        1 tttttgttta aacgacctaa aataacagca attcaatacg ccacacaaat atattcataa
       61 gcgtgataat tcacccctat tacatcctcc aaaaactttg agtaatatcc gctcgcaata
      121 ttaattttat ataatttttg taaggatgca tgtaatgaaa aaagcacttc tcgcagccgc
      181 tctggttatg gcttctggtt ccgccctggc tgtagatggt ggtcatatcg actttaacgg
      241 tatggtacag tccggtacct gtaaagtggg tgtggtagat actggtatgc acagcgttac
      301 cactgatggc gtggttaccc tggatactgc gaatgttact gatacttttg ctgaagttag
      361 cgcaactgct gtcggtttgc tgccgaaaga gttcatgatt tctgttgagt gtgatccagg
      421 tgctccgaag aatgctgaat tgaccatggg ttctgcaagc tacgcgaaca ccagcggtac
      481 cctgaataac aatatgaaca tcactgttaa cggtattcaa ccggctcaga acgtgaatat
      541 tgctgttcat aacatgaaaa acaaagctgg cgctgctgaa attaagcagg tccatatgaa
      601 caactcttct gaagttcaag aactgacttt agacgcagag ggtaaaggcc agtatgtgtt
      661 taacgcatct tacgttaaag caccgaacag ccaggctgtt actgctggtc atgtaactac
      721 taacgcactg tacaccattg cttataagta attgatgacg tattgagcat aaggtttgcc
      781 gccaggcaaa ccttatattc gcgaatttaa tctttctaca gacagcaagg agtgcttatg
      841 tttcccttgg ttaaaaaaac ggttagtgca ttatttgtaa gtactctgtt agccagtgcg
      901 cctgcattcg ctgacattat tatttccgga actcgtatca tttataacgc tgataaaaaa
      961 gatgttaacg ttcgtctgga aaataaaggg aatcgcccct tactgattca aaactggttg
     1021 gatactggcg atgacaatgc cgatcccagc cagatcaaag tgccgtttac cgcaaccccg
     1081 cctgtttcac gcgtggagcc aaaacgcggt caaacagtaa aagtgatgta caccggtgct
     1141 acagccctgc cgaaagatcg tgaaagcgtc tactggttta acgtgctgga agtgccgcct
     1201 aagccgaaag atgctgaggc tgacaaaaac ttgttacagc tggctttccg tacgcgcatc
     1261 aaactctttt atcgcccgag cggtttacag ggagaaccgg ctgaagcacc ggcgaaaatc
     1321 acctggaaat taaacaacac tcagttacag gcaaataacc caacgccata ttacgtgtcc
     1381 tttaatgagg tcaagctgga aagcggcggc aaaacctaca acgtcaattc cagcatggtt
     1441 gcgccgttca gccaggcaag ctttggcgta accggtctgc caggaagcgt ttcatccggg
     1501 aaagtggtct ttaaagcgat caacgacttt ggcggcaaca tcgatggttc cgcaacattc
     1561 taaaaaataa acggattctt tttaacatcg ccaggaatgc aaccttgtac cagtttactc
     1621 atcaaaaaag ccgtatcccg aaaaaaacgc tacttgcggc ctgttgtgcc ctattttata
     1681 gcagcaacgg tgctgcggca gacacggtgg aatatgacag ttccttctta atgggaactg
     1741 gcgcatcaac gattgatgtt aaacgttatg ctcaaggcaa cccgacaccg ccgggtctct
     1801 ataatgtccg cgtgtttgta aacggtcagg cgacgtccag cttagaaatt ccgtttgtgg
     1861 atattggcga aaacagtgcg gcggcctgtc ttacccgcaa aaacctggcg cagctacaca
     1921 ttaagcaacc agaacagcct gtcactttac tcgccagaga aggtgaagaa gaggattgtc
     1981 tggatctggc aaagtcatac gataaggctg acgttcgctt tgatggcagt gaccagtttc
     2041 tcgatctgac tgttcctcag gcttatgttc tgaaaaacta cggtggctat gtcgacccct
     2101 cactgtggga gtcagggatt aacgctgcca cactggcata taccctgaac gcatatcaca
     2161 caagttcaga taacgacaat agtgacagcg tttatggcgc atttaactcg gggatcaatt
     2221 taggagcctg gcacttccgt gcgcgcggta actataactg gacaacagat aacggcagcg
     2281 atttcgattt ccaggatcgt tacttacagc gtgacattcc ggcaatccgt tcccagataa
     2341 ttatgggtga tgcctacacc accggtgaaa catttgactc tgtcaacgtc cgtggtgttc
     2401 gcctgtacag cgacagccgt atgctgcctt ctgcgctggc cagttacgcc ccgaccatcc
     2461 gcggtgtggc aaactccaac gccaaagtta cggtgacgca gaacggttat aaaatctatg
     2521 aaactaccgt tccacccggt gaatttgtta tagacgacat tagcccctcc ggctttggta
     2581 gcgaactggt cgtaaccatt gaagaagcgg atggttccaa acgcaccttt acgcaaccct
     2641 tctcgtcggt tgtgcaaatg caacgtcctg gtgtaggtcg ttgggatttc agtgccggta
     2701 aagtcattga tgatagcctg cgttccgaac ccaatatggg gcaagcctct tattactatg
     2761 gtctgaataa cctcttcacg ggttataccg gcattcagtt caccgataac aactatcttg
     2821 ccgggctgtt aggtgtgggt atcaacacca gcatcggcgc ctttgcggta gacgttaccc
     2881 attcccgtgc tgaaattccg gatgataaaa cctaccaggg gcaaagttat cgcgtgacct
     2941 ggaacaaact tttccaggat accgggacat catttaacct cgcggcgtac cgctattcca
     3001 cccaggatta cctgggcctg catgatgcgt tagtcctcat tgacgacgcc aaacatttgt
     3061 ctgccgatga agacaaaaac accatgcaga catactcacg tatgaaaaac cagtttactg
     3121 tgagtattaa ccagccattg aatatcgcct atgaagatta cggttcactg tttatttccg
     3181 gtagctggac agactactgg gcagcgaaca atagccgcac tgaatataat gttggttaca
     3241 gtaaaagcgt ttcgtggggc agcttcagcg tcaacctgca acgtagctgg aatgaagacg
     3301 gcgctaaaga tgacgcaatg tacgtcagcg ttagcgtgcc tattgagaat attttaggtg
     3361 gcaaacgtaa gtcttctggt ttccgcaatt taaatactca gctcaatacc gatttcgatg
     3421 gttcacatca gttgaatgtt aacagttccg gtaacactga aaacaatctg gtgaactaca
     3481 gtgtcaacgc aggttatagc ctcgataaaa actccggcga tttagcctct gttggtggtt
     3541 atctcaacta tgaatcgggg ttaggcggta tttccgcttc ggcttctgcc acgtctgata
     3601 atagccagca gtactccatc tctaccgatg gcggctttgt gttacacagt ggtggtttaa
     3661 cgttcactaa caacagtttc agcagtaacg acacgctggt gttaatcaac gccccaggtg
     3721 ctaaaggcgc acgaatcaat aacagtaata acgaaatcga tcgctgggga tatgccgtga
     3781 cgtcctctgt cagcccctac cgtgaaaacc gggtaggtct gaacattgaa acactggaaa
     3841 acgatgttga actgaaaagt accagcgcca ccaccgtacc acgtagcggc tccgttgttt
     3901 tgacccggtt cgaaactgac gaggggcgtt ctgccgtgct gaatattact gccgccaatg
     3961 gtaaatccat tccgtttgct gcggaggttt accagggtga cgtgatgatc ggcagtatgg
     4021 gtcagggtgg tcaggcattt gtgcgcggta ttaacgacag cggggaatta acagtgcgct
     4081 ggtatgaaaa caaccaaacc attgactgta agttgcacta ccagttcccg gcccagccac
     4141 aaacgctggg aagtaccaac accttattac ttaacaatct tacctgtcag gtagcaaatc
     4201 actaatatgg aaaattctat gaaaaggata ctactaacat ccgcgttaat aggtctgggt
     4261 ttaccggcgg ttggctctgc aacagatctt aatgtcgatt ttacggccac cgtattagct
     4321 accacttgta cgataaccat cgtacaagat ggtggtccag tagtgacggg tagtaatggt
     4381 gaatacagcc tgacaattcc tgatgttggc ctggataagg ttgtcactgg agatgttgca
     4441 gctcaagcca actttaaatt actggcttcc gattgcagca atggttactc aaaaattttt
     4501 accacactta caggtgcatc tgtttcaggc aagttgattg tcaatgaagc tagttctggc
     4561 gcagcgggtg tggggatggg tattaagcgt agagatgccg cagattcggc tttcttcacg
     4621 cccaataata ctgacaaatt tgaatggtct actgatgaga aaactaacgg tgtgcctttg
     4681 accgtcgcac tgcgcgaaac aacggcagga gcgggacgca ccggtgcgtt tcaggcaaaa
     4741 gccaccttta acttcaccta cgaataacag gaacagccac catgaaatta aaagtcatcg
     4801 ctacactgat tgctactgtt gccgtgggtg taagctttaa cagcaatttt gcttctgcga
     4861 gtacaacgtc cgcttcttta accgtaaaca gtaacctgac tatgggtacc tgcagtgctc
     4921 aggttctgga ttccagcaat aaagcgacag ataccgttgc cgtgggcaat gtttatatct
     4981 ctgagttagc ggcaaaaagc aaagtgcaac cattcaaaat ccgcttcagt gattgtgccg
     5041 gccttccagg aaagagtgct caactcgtct tagcgcccag gcctggtacg aattgcgctg
     5101 gcggccagtc taacactgcc ggctttgcaa attcaatttc ctccgccaat ggtggctcaa
     5161 gcagagcagc ggtcgaagta tggacgacaa cgacccctga gggaagtgga agccagcaat
     5221 tcagttgtgt acaacgaaac gtcataacag tcgatctttc taatgccagc acaacacagc
     5281 cttatgatta cgatttaagt gcacggatga ttatcgctga ggggagaacg gttaatgatg
     5341 tattaccggg tgagtttctt tctcccacca ctttcactat cacttatcag taatagcgga
     5401 taaggatatt agaatgctat gcaggcatca taaattggta cgctttttag ggctggtagc
     5461 agcccttatt acgcctttcg gctatgccgg tcaggatgtg aatctcacgg cgctgattgt
     5521 ggccagcacc tgccaggtgg aagtcagtaa taatggcgtt gtcgatctcg gcacagtgac
     5581 gctggactat tttgctgaca atgtgacccc gacaacggat tatgcgggtg gcaaaacgtt
     5641 taatgttaat gtggtctctt gcgataacat tcaaacgaca cagtcgcaaa tgaaattaga
     5701 ttttcagccg caggcgggat cgcttgcgca ggtgaataat caaattttta gtaatgaata
     5761 tgaacagcag gctaccgggg cgaaaaacgt cggcattgtg atattttcgg cccagccgaa
     5821 tcagcagaca tttaatgtgc gaggaacgga tggttcctca acagcaattt attcggtcgc
     5881 gcctggcaat gctgttccct caacctggac cttttattct cgtatgcagc gggtgaataa
     5941 tgcccttcct cctgaatcgg gaatggtgag aagccaggtt attgttaacg tatcttacga
     6001 ataataagga taattatgca aaggaaaggc aataatctgt taattcagtt atgcagtgcg
     6061 atactgctat tttttaccac atcctggtat gcattggcga atgaatgtta tatagaaagc
     6121 aatgctgaag ggtcttatga tatggtgata aacccaacac ccttcagtat acagacacga
     6181 atggttgatg caccagagga aatagccttc gcgacctggg attctcatat taatcttcgg
     6241 ggcgataaga ttggttgtaa atccttaggc agtaacacga gctctgtgca ttttcttaat
     6301 actgctgatg ccagtctctt atcaacctat actaccagcc gtggcgatgc tttattgaaa
     6361 acaaccgttc ccggtattgt ttattcagta gaattagtat gtaaaagttg tggagccgcg
     6421 gaggaacttg atttgtatct tccagcacaa agtggtgctg ataatcatac agataacgcc
     6481 agcgcaaagt gggcttatga gtatagtgac gatagctggt atttacgatt tcgttttttc
     6541 tgggccccgg aatttaaacc tcaaactggg gtttcagaag gaactgcgat gccaggtaaa
     6601 atagcttcct ggtatatagg caataatgat caaccatgga taaatttttt cgttgataca
     6661 agctccgttc atttttacgt tgacgaaccg acctgtgcaa cagttgccct ggcacaagat
     6721 cagggcaacg tcagtggcaa ccaggtaacg cttgggaaca gctatgtatc ggaagtgaaa
     6781 aatgggctta cgcgggaaat ccctttttct atccgtgctg aatactgtta tgccagtaaa
     6841 attacgatta agttgaaagc ggcaaataag cccagcgacg gcacactggt aggtaaaaca
     6901 acgggctctg cttccggcgt ggctgtaaaa gttaattcaa cttacgacaa tagcaaagtt
     6961 ttgttgaaag cagatggtag caacgcggtt gactataact tcgccatctg gtcaaacaac
     7021 ctgctgaatt tcccctttac ggcacagttg gtaccggatg gcagtggtgc cgctgtcggt
     7081 gttggaacat tttcaggtaa cgcgaccttc tcctttacct acgaataagt aaaacgtcat
     7141 tccctccccc atcgcaccag agttctgctc tggtgcgaac attttcccca cactaaaaac
     7201 cattgccccc ctaatttcga ctgttagaat gcgatcaatc tgactttagc tatcaggata
     7261 cgttatgaaa ccgaccacta ttgcttcatt gcagaaatat aagcaggaaa aaaaacgctt
     7321 cgccaccatc actgcctacg actatagctt cgccaaactc tttgccgatg aaggacttaa
     7381 tgtcatgctg gtaggcgact cgctgggcat gacggttcag gggcacgact ccaccctgcc
     7441 agttaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgtggcg cgccaaactg
     7501 cctgttgttg gctgacctgc cgtttatggc gtatgccacg ccggaacaag cttttgaaaa
     7561 cgccgcaacg gttatgcgtg ccggtgctaa catggtcaaa attgaaggcg gtgagtggct
     7621 ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc acttaggttt
     7681 aaccccacag tcagtgaata ttttcggtgg ctacaaagtt caggggcgcg gcgatgaagc
     7741 gagagatcga ctgcttagcg atgcattagc cttagaagcc gctggtgcac agttgctggt
     7801 gctggaatgc gttccggttg aactggcaaa acgtattacc gaagcactgg cgatcccggt
     7861 tattggcatt ggcgcaggca acgtcactga cgggcagatc ctcgtgatgc acgatgcgtt
     7921 cggcattacc ggcggtcata tccccaaatt tgccaaaaat ttcctcgctg aaacgggcga
     7981 catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt atccgggcga
     8041 agaacacagt ttccattaag gagtgacgtt gtgttaatta tcgaaaccct gccgctgctg
     8101 cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgtg tggcgctggt acctaccatg
     8161 ggcaacctgc atgatggcca catgaagctg gtcgacgaag ccaaagcccg cgccgatgtg
     8221 gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggagga cctggctcgt
     8281 tacccacgga ccttgcagga agactgcgaa aagctgaaca agcgcaaagt ggatctggtt
     8341 ttcgcgccct cggtaaaaga gatctacccg aacggtactg aaacccatac ttacgttgac
     8401 gttcccgggc tttcgaccat gctggaaggc gcgagccgtc cggggcattt tcgcggcgtt
     8461 tcgactatcg tcagcaagct gtttaacctg gtccagccgg acatcgcctg cttcggtgaa
     8521 aaagattttc agcaactggc gctgatccgc aaaatggttg ctgatatgag ctttgatatt
     8581 gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcactgag ttcccgtaac
     8641 ggttatctga cggcagaaca acgcaaaatt gcgcccggtc tgtacaaagt tttaagttcg
     8701 attgccgaca aattgcaggc tggcgaacgg gatctcgatg aaattattgc tattgccggg
     8761 caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga tgccgacacg
     8821 ttgctggaag tctctgaaac cagcaaacga gctgtgattc tggtagccgc ctggcttggc
     8881 gatgcccgcc tgatagacaa caaaatggtc gaactggcgt aatacttaac tggcgctacg
     8941 gctgatggcg ccagttatta atttgcccta cgtcatggcg atgacgtctg taagtgatta
     9001 ttcatatcgc tggcaattta gttagagtta attagcttat caatctctgt aagaggtaaa
     9061 gttgtcacct ctgcaacgtc ttcccgagac atccctttac ttagaaaacg ctgggcgaat
     9121 tcctgtctta ccttctggag ttcctcttga tatcctttct gaaatccctt ctcttcaaac
     9181 cactgcgcca acgtctgcct ggtttctctc cgtgtcttta tttccatagg ttcccttaat
     9241 tatctggtca gttaacaata acaattatct ggctataact gatgatgcag acaacttagt
     9301 taatcagctt atcaatctcg gcaaggggta aatttgccat ctctgcaacg tcttcccgag
     9361 acattccttt attcagaaga cgcaggacga attcttgcct ttcctcctgt cttcccctct
     9421 cttcaaacca ctgcgccagc gtcatcatag actcccctcc cgtttccctg tctctcaaca
     9481 caccgtaaaa caaatccgct tgttcagtat gaccgcgttg cagcatatag ttttgcatgg
     9541 caactaactg acttccgcta gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca
     9601 ataacattaa gtcgcgctgg cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc
     9661 gatgttgcat gatttcgtca tccggtgtga tagtgatatc caccagcggg aaaggactgt
     9721 tatagacgcg tcgcgccagc tcaggcgagt aaaacatatc aaaccagcac attgagagcg
     9781 gataaggtgt ggcctcgccc tgataaaaca gtatcggcac caccagcggc agcttatcgt
     9841 ggtcagcctc cagatgccgg tgcatggcgg caatagaata acgcatcatg cgaaaagcca
     9901 ttttcttatc tggcttgctt tgatgttcaa tcactacatg cagataaccg ggattgccct
     9961 gcatttgcac ggaatagagc acgtccgtgc tgtgtccttt caggctctct tcaatgaaac
    10021 tccccgactc taaatgaagc gtattgaggt cacaaagttc gcgtaattcc actggcaaat
    10081 gtatctccag aaagtcgcga gccgtctccg catgcattaa aaattgttta aataccgcgt
    10141 catgcggtgt ggtactcggt gcatccatct tccctgtctt ccgtgttgtc ttgcgtgaag
    10201 gcagtatcca gtcaaggcgt gctatcgtca gccacctctt tattgtttac cgagcagcgt
    10261 tcagagaaat atcaattcgt tacaggcgat acatggcacg cttcggcgcg gtacgcaaac
    10321 tgctgatgca aatgccgtca gatgagtaga cactaaacaa aaatcgggca atactgcgtg
    10381 agaatttcct caaagcaggc accgcctgct tcgttaacga cagggtagaa aggtagaagt
    10441 tatgattcgc acgatgctgc agggcaaact ccaccgcgtg aaagtgactc atgcggacct
    10501 gcactatgaa ggttcttgcg ccattgacca ggattttctt gacgcagccg gtattctcga
    10561 aaacgaagcc attgatatct ggaatgtcac caacggcaag cgtttctcca cttatgccat
    10621 cgcggcagaa cgcggttcga gaattatttc tgttaacggt gcggcggccc actgcgccag
    10681 tgtcggcgat attgtcatca tcgccagctt cgttaccatg ccagatgaag aagctcgcac
    10741 ctggcgaccc aacgtcgcct attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc
    10801 gattccggta caggttgctt gattgttatc cctgcggctg gttactcacc agccgcgaca
    10861 tcgtctccag cgaatccgtt cttaagatat aaagtcgttt tagtaacaac ggattatccc
    10921 ccggttttac tttgcctttc atggttgtca ccgccaggtg aaatccggca tcgtttgctg
    10981 ccttcacggc tttgtcatta aatccgccaa acggatacga aagataccag acatgcggat
    11041 taaattgcgc cagagcgcgg cgtgaacgtg caaaatcaaa cagaatattg tgctcactac
    11101 gactcagtaa tatggggcgg cgatagccat ccacccgatg caaaaaatgg gtatgtgact
    11161 gaaaatcaaa gacatcgcga atttcgttaa gctcagaaac gctcataaat tgcagcgatt
    11221 ttgggttcca cttctgcggg tgacgtttga tgcgcgaggt aacaataaac gccgtcgcct
    11281 tcatgccata ttgtttcaac acaggatacg catagcggct caccgacttg aggccatcat
    11341 caaaggtaat caccaccgct cgcgcaggga gattgatctt attcttcacg taaccttcca
    11401 gctgcaccat gctcagtgtc gcgtatcccc tgtcgcgcag ccaggccatc tggttattga
    11461 aagcgcgtac cgaggtggtc gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca
    11521 gaatatggtg ataagttagc accggaaggc cattatcggg ttgggcatcc agcgcgctga
    11581 tataggccag tcgatcgcca atacggacct gataccaggt ttgatttaac ctgtctttca
    11641 gtttatgcaa aatcgggtag cgcaaattgt ccgccagtac cccaaatggc gcactgcccg
    11701 cactcggcgc gttatagacc ggcgtatctt tccaggtaac taagttctga ttactcagcg
    11761 gcttgttgag gtcacccaaa ccgtcttcaa ctttttgtcg cccctgaacc ggctcgagat
    11821 gccctttatc gataaaccct ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag
    11881 tgggctccac ggcaatgatt tgtccggccc gaatattccc cacggtcacc atcttgtcgc
    11941 caatttgcgc ccagaccgca gcattttcga tggtttgcat ataacgggca ggtaacgcgg
    12001 cactgacact tgcggtaaac agcatcagca ggagaataac agcttgtttg tacatgaaaa
    12061 aataaccgag ggtgaaacaa cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt
    12121 aatactcata caaattatgt ttgaggacga aaggcggatt tttttgttgc tggtgccaaa
    12181 gactactcac ctccggccca cccagttcga caatacgctc gcgaaactct gaactggtca
    12241 tggtttcacg gcaagccatc atctgttcaa ttaacggtaa cgtgacacca gaaatcactt
    12301 cgcaacggga gtgtttgtgg cttaataatg aagccacgcg atacggtggc gctccggcta
    12361 tatcggttaa gaagatgaca ccctcaccag agtccgcttc atgcagagca tcgcacatca
    12421 tgcggctgag catattagaa cttaatccgc gccagaaatt cacggcccgg cactgaagaa
    12481 gcgccccatg ttttttctcc agcgcatcca gtatctcttg cgcccggtca tcgtgacagg
    12541 taattaccca acctaacatc gccttcctcc ttagccgaaa atcagtctac cctgaccatt
    12601 gttgataaag ggtgatagcc gtcaaaactc ctctccccca aatccagagg agaggaaata
    12661 gccttagcta cgcaaaccac gtccacgttg gatcagcgac caacagatca aataaaacgc
    12721 cacaataaag accaccagta cgccaaaggt agtgaccagc ggaacatcat tgataccgag
    12781 gaagccgtag cggaaaccac tgatcatata aacgattggg ttcaggtgcg acagcccttg
    12841 ccagaacggc ggcaacaaag tcagtgagta aaagacccca cccaaatacg tgagtggcgt
    12901 taacacaaag gttggcacca ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag
    12961 caaacccgca agggagaaca acaccgccgt gagcaccagg gttaaggcaa cgaataccca
    13021 cgaatgcacc tgaaacggca caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa
    13081 cagaccacgc gccacgccac cgccgacata tccggcaata atgacgtgag tcggaaccgg
    13141 cgctaccagc agctcttcaa tattacgctg gaatttggca ccaaaaaatg atgacgcgac
    13201 gttggcgtag gcgttggtga tcaccgacat catgatcagc cccggtacga tgaactgcat
    13261 gtagctgaag ccatgcatat cgccaatacg cgaaccaatc aggttaccga aaataataaa
    13321 gtaaagggtc atggtgataa ctggcggcac cagcgtctgc acccagatac gcataaagcg
    13381 atggatctct ttcgcccaga tgctttttag cgccacccag taaagatgca tcatgcgcga
    13441 tctccttgtt tttcattaac cagtgaaaca aacagctctt ccagacggtt agctttgtta
    13501 cgcatactta atacctgaat gccctgctca cttaactgcg taaatacgct gttgatcccc
    13561 tgctcacgca gcacttcaac ttccagcgtc gcggtatcca ccagccggta ctgatagcca
    13621 tcgagcttcg gtaacggact tttcggtgcg agatcgagaa taaaggtttc cgatttcagc
    13681 ttcgccagca gcgccttcat cgaggtattt tccaccagct caccgtgctg aataatgccg
    13741 atattgcgac acagcatttc tgcttcttcc aggtagtgtg tggtgagaat gatggtggtg
    13801 cctttgtcgt ttaaatcctt caaaaagccc cacattgagc ggcgaagttc aatatccacg
    13861 cctgcagtcg gctcgtcgag aatcagtagt ttaggttcat gcattaacgc acgggcaatc
    13921 attaaacggc gcttcatccc gccagataac atgcgcgcac gttcgttgcg ttttccccat
    13981 aaatcgagct gcttaagata tttttcgctg cggatgtacg cttctttgcg ctccacaccg
    14041 tagtaccctg cctgattcac cacaatttgc tgcacggttt caaacgggtt gaagttaaat
    14101 tcctgtggca ccagtcccaa ctgacgttta gcgttcacga catctttctc gagatcgtaa
    14161 ccaaatacgc tgacccgccc ggaggtttta tttaccagag agctgataat accgatagtg
    14221 gtcgatttcc cggccccgtt cggcccgaga agcgcataaa aatcacccgc ttcgacctgc
    14281 aaatctattc cacgaagcgc ctgaacgccg cctggatagg tttttttaag ctgttgaagt
    14341 tccagtgcaa tggtcataaa tttttactta ccttacgttc ttacacttta tctgtggttt
    14401 aaatcgtccc ggagttgccc tatattagcc aaacgtaatt atttggttac aggtcgttaa
    14461 cctccatgaa agacatagat acactcatca gcaacaatgc actatggtca aaaatgctgg
    14521 tggaagagga tcccgggttt tttgagaaac tggcacaagc gcaaaaaccg cgctttctat
    14581 ggattggatg ttccgacagt cgcgttcctg cagaacgttt aaccggtctt gagccgggcg
    14641 aactctttgt tcaccgtaat gttgctaacc tggtcattca caccgacctg aactgccttt
    14701 ccgtggttca gtatgcagtg gatgtactcg aagttgaaca cattattatc tgtggccact
    14761 acggttgcgg cggcgtacaa gccgcagttg aaaacccgga actggggctt atcaacaact
    14821 ggctgctgca tatccgcgat atctggttca aacatagctc attgctcggc gaaatgccgc
    14881 aagagcgccg tctggatacc ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg
    14941 gccactccac cattatgcaa tcagcgtgga aacgcgggca gaaagttacc attcacggct
    15001 gggcctatgg cattcacgac ggcttgctgc gtgatctgga tgttactgcc accaaccgcg
    15061 aaacccttga gcaacgttac cgtcatggga tttccaacct caagctgaaa cacgccaacc
    15121 acaaataaaa atgccatgcc ggatgcaaca catccggcaa cttcacactt actcgtccag
    15181 cagaatcact ttgccgatat acggcagatg acgataacgc tgtgcgtaat caataccgta
    15241 acccaccaca aactcatccg ggatcgagaa accgataaat tctaccggga cgttcacttc
    15301 acgacgggac ggtttatcca gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct
    15361 taagatctca cgcactttcg acagtgtatt ccccgagtcg atgatatctt caacaatcag
    15421 cacgtccttg ccacggatat cttcatccag atctttgagg attttcacat cacgggtggt
    15481 ggacatgccg ctaccgtagc tggaggcggt cataaagtcg acttcatgag atacctgaac
    15541 ttcacggcac aggtccgcca taaacataaa tgagccacgc agcagaccca ccagcaccat
    15601 atcgctgccg ctgtctttgt aacgctcagt aatctgacga cccagttcgg cgatacgcgc
    15661 tttaatctcc gcttcgggga tcattacttc tacagtatgt ttcatatctc taaccatatg
    15721 atttaaaaac aaatcacttc gcacgggcag gttttggcac cgcaacaaag cgcaagacat
    15781 gcagtatacc agtaaaacta ttcattcgcg gaatctgtta ataaagctcg aattgtgatg
    15841 acggtcacac atgttaaaac ctataattaa ttgctactaa aatattaatg aattgaaatg
    15901 gtgtctcttt atggcaatta acaatacagg ctcgcgacga ttactcgtca cgctaacagc
    15961 cctttttgca gcgctttgcg ggctgtatct actcattggc ggaggctggc tagtcgcgat
    16021 tggcggctcc tggtactacc ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct
    16081 gtggcgcagt aaacgtgccg cgctttggct atacgccgcc ctgctgctcg gcaccatgat
    16141 ttggggcgtc tgggaagttg gtttcgactt ctgggcgctg actccgcgca gcgatattct
    16201 ggtcttcttc ggcatctggc tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc
    16261 cagcggcgca gttgccgcac tggtggtcgc actgctgatt agcggtggta tcctgacctg
    16321 ggccggattt aacgatccgc aggaaatcaa cggtacctta agcgccgatg ccacacctgc
    16381 ggaagctatc tcacccgttg ccgatcagga ctggcctgcc tatggtcgca atcaggaagg
    16441 tcaacgcttt tcacctctga aacaaattaa cgccgataac gtccataatc tgaaagaagc
    16501 ctgggtgttc cgtactggcg atgtgaagca gccgaacgat ccgggcgaaa tcaccaatga
    16561 agtgacgccg attaaagtgg gcgacaccct ttacctgtgt accgctcacc agcgcctgtt
    16621 tgcgctcgat gccgccagcg gcaaagagaa atggcattac gatcctgagc tgaaaactaa
    16681 cgagtctttc cagcacgtaa cctgccgtgg cgtctcttat catgaagcca aagcagaaac
    16741 ggcttcgccg gaagtgatgg cggattgccc gcgtcgtatc attcttccgg tcaatgatgg
    16801 tcgactgatt gcgattaacg ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg
    16861 cgtgctcaat ctgcaaagca atatgccaga caccaaaccg ggtctgtatg aaccgacttc
    16921 gccaccgatt atcaccgata aaaccatcgt gatggccggt tcagttaccg ataacttctc
    16981 aacccgcgaa acgtctggcg tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg
    17041 ggcttttgat cccggcgcga aagatccgaa cgcaatcccg tctgacgaac acacctttac
    17101 ctttaactcg ccaaactcgt gggcaccagc ggcctatgac gcgaagctgg atctggtgta
    17161 tctgccgata ggcgtgacca cgccggatat ctggggcggt aaccgcacac cggaacagga
    17221 acgttatgcc agctcgattc tggcgctgaa tgccacaacc gggaaattgg cgtggagcta
    17281 ccagaccgtt caccacgacc tgtgggatat ggatcttccg gcacagccga cgctggcgga
    17341 catcaccgtt aatggtcaga aagtgccagt tatttacgct ccggcgaaaa ccggcaacat
    17401 ttttgtgctc gatcgtcgta atggcgaact ggtggttccg gcaccggaaa aaccggttcc
    17461 ccaaggtgct gccaaaggcg attacgttac ccctactcaa ccattctcgg aactgagctt
    17521 ccgtccgacg aaagatttga gcggtgcgga tatgtgggga gccaccatgt ttgaccaact
    17581 ggtgtgccgc gtgatgttcc accagatgcg ctatgaaggc attttcacgc cgccatctga
    17641 acagggcacg ctggtcttcc cgggtaacct ggggatgttt gaatggggcg ggatttccgt
    17701 tgatccaaat cgtgaagtgg cgattgccaa cccaatggca ctgccgtttg tttcgaaact
    17761 gatccctcgc ggtcctggta acccgatgga gcagccgaaa gatgccaaag gcacgggtac
    17821 ggaatccggc attcagccac agtacggtgt accgtatggt gtcacgctca acccgttcct
    17881 ctcaccattt ggtctgccat gtaaacagcc agcatggggt tatatctcgg cgctggatct
    17941 gaaaactaat gaagtggtgt ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt
    18001 cccgatgcct gtaccggtgc cgttcaatat gggtatgccg atgctgggcg ggccaatctc
    18061 cacggcgggt aacgtgctgt ttatcgccgc tacggcagat aactacctgc gcgcttacaa
    18121 catgagcaac ggtgaaaaac tgtggcaggg tcgtttacca gcgggtggtc aggcgacgcc
    18181 aatgacctat gaagtgaatg gtaagcagta tgtggtgatc tccgcaggcg gtcacggttc
    18241 atttggtacg aagatgggcg actatattgt ggcttatgcg ctgccggatg atgtgaagta
    18301 agacttgctc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgcgaacgcc
    18361 ttatccagcc tacaaaattg tgcaaaatca atggattgca caggtagcgt aggcctgata
    18421 agcgtagcgc atcaggcaga tttgcgtttg tcatcagttt aatgcccgga gagatccggg
    18481 catatttccg aatacggtct ttttataccg taaaccctaa catcatcccc gtatcttcat
    18541 gctccagcag atggcagtgc gccatataag catgttcttt cggtgcatcg tgattaaact
    18601 tcaccagcac ttcgctgaca ttaccctcta ccttaacggt atctttccag cccgcgcgat
    18661 gagccgctgg cggtttgcca ttttctgaca agatacggaa ctgcgtgccg tgaatatgga
    18721 acggatgcag catcatgtcg cccacgccag agataaccca acgttcgtac tgccctttcg
    18781 ccgccgcaaa catcggctta ttcatatcaa acgcctgacc gttgatttta ttggcatggt
    18841 ggaaatcgaa cttcccgccg tggttcatat gattcatatt gccgtgcccc atatggccca
    18901 tcatctggct gtgatccatt ccggccatcg cctgatcgcc atatttctcc attagcatct
    18961 gcatccccat catatcgagc atcgggtcca tagaaagttg cagcttgcgt accgtcagcc
    19021 cttccagcga aggtaacgca ggcaggctac ttaatgtgtc tggcaaagca ccggaggcac
    19081 taatagcaat cggctgaatc cgcattaccg gatgcggctt gtcaaacggc gcaatcgcca
    19141 tccccatctg gctgaccggc agcgtcacca ggtcaaaggg tttgttgtcg ttaacctcca
    19201 ccagcacttc aaaacgctcg cccatcagca ccggcagctc gttcaccttc accggttcag
    19261 gtagcagacc accgtcgctg gcaatcacat aaagcgggcg attgtcgctg gtggcgaaat
    19321 tgagcgagcg ggcattacag ccattgagca aacgcaggcg cagccaacca cgcggggcag
    19381 cgtgttgcgg gtagattgca ccgttggtca gcaacgtatc gccaaaccag cccacggcgg
    19441 cggtcatcac atccagttga taatcaatct gcccgtcggc gttaaatttc ttatcctgaa
    19501 cgatcaccgg aacatcatcg ataccccact gttttggcag cattaatttc aggatctcgt
    19561 catcttcaat caccaccaga ccagccagcc ccatcgccac ctgtcgcccg gtcttgccat
    19621 gttgatgtgg atggaaccag caggtagcgg caggttgatc aacgttcaac gtcaccgagc
    19681 gcttgccacc tggcggaata attccctgtg ggccgccgtc gacttcaccc ggtacttcca
    19741 gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt atagatatca accgttaccg
    19801 ctttgccgcg ctgtaatttc accgccggcc ccagcagatt gccgttatag ccccaggtag
    19861 ttgcagtttt ctcgccaaag gtagactgac ctgcgccaat agttaactga atgcgattac
    19921 gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg gcgttccgcc gcaaagactg
    19981 cacggctcca cagtggcaag gctgaagcca cacccagtgc gacggaatat ttcaagaaat
    20041 cacgacgttg catagttatt tccttattct taagcaggcg aaacaaaatc aaacgttgag
    20101 catagtcctt ccccttacgg gaaggtcaag ccgcagacac aatttaatcg acaataatgg
    20161 tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg tagaagcaat
    20221 gaagacgttt ttcagaacag tgttattcgg cagcctgatg gccgtttgcg caaacagtta
    20281 cgcgctcagc gagtctgaag ccgaagatat ggccgattta acggcagttt ttgtctttct
    20341 gaagaacgat tgtggttacc agaacttacc taacgggcaa attcgtcgcg cactggtctt
    20401 tttcgcccag caaaaccagt gggatctcag taattacgac accttcgaca tgaaagctct
    20461 cggtgaagac agctaccgcg atctcagcgg cattggcatt cccgtcgcta aaaagtgcaa
    20521 agccctggct cgcgattcct taagcctgct tgcctacgtc aaataatccc tgataccttt
    20581 ttgtagaaat attgaccgtg catccgcggt caatgttagc tattatgttg cgcccttttt
    20641 ttacgggtgt taacaaagga ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac
    20701 gctacacgac cagtttgggc agtactttgc ggtagataac gttctgtatc atgaaaagac
    20761 cgatcaccag gatctgatca tttttgagaa cgctgcattt ggtcgcgtaa tggcgctgga
    20821 tggcgtagta caaaccaccg agcgcgacga gtttatctat catgagatga tgacccatgt
    20881 tccgctactg gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc
    20941 catgctgcgt gaagtaaccc gacataaaaa cgttgagtca atcacgatgg tggaaatcga
    21001 tgcgggtgtc gtgtcgttct gccgtcagta tctacccaac cataacgccg gtagctacga
    21061 cgatccgcgc tttaagctgg tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca
    21121 gacctttgat gtcattatct ccgactgcac cgatcctatc ggtcccggcg aaagcctttt
    21181 cacttcggca ttttatgaag gctgcaaacg ttgcctgaat cctggcggta tcttcgtcgc
    21241 acaaaacggc gtctgctttt tacagcagga agaagccatc gacagccatc gcaaactcag
    21301 ccattacttc agcgacgttg gcttttatca ggcggcgatc ccgacctatt acggcggtat
    21361 catgactttt gcatgggcga cagataacga cgccttacgc catctctcaa ccgaaattat
    21421 tcaggcgcgt tttctcgcct ctggcctgaa atgccgttat tacaatccgg cagtccatac
    21481 ggcagctttt gccttacccc agtatctgca agacgcactg gcttcacagc cgtcctaagg
    21541 agaagataag aaattgaaaa aactgaaact gcatggcttt aataatctga ccaaaagtct
    21601 gagtttttgt atttacgata tctgctacgc caaaactgcc gaagagcgcg acggttatat
    21661 tgcttatatc gatgaactct ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg
    21721 ttccattatc ggggctaata ttcttaacat cgcccgccag gattacgaac cacagggtgc
    21781 cagcgtcact attctggtga gtgaagaacc ggttgacccg aaactcatcg acaaaacaga
    21841 acaccccggc ccactgccag aaacggtcgt tgcccatctc gataaaagtc atatttgcgt
    21901 acatacctac ccggaaagtc atcctgaagg cggtttatgt accttccgcg ccgatattga
    21961 agtctctacc tgcggcgtga tttctccgct gaaggcgctg aattacctga tccaccagct
    22021 tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt tttacgcgcg acattaacgg
    22081 tatgaagcac tttatcgacc atgagattaa ttcgattcag aactttatgt ctgacgatat
    22141 gaaggcgctg tatgacatgg tggatgtaaa cgtctatcag gaaaatatct tccataccaa
    22201 gatgttgctt aaagagttcg accttaagca ctacatgttc cacaccaaac cggaagattt
    22261 aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta
    22321 ttacgggcgc aatatgccag ctgtttaacg gctctggcgg agctcccagg ctccgccaga
    22381 tttatttact tctgctgcac gaaattgcgg taagccgcca ccacctgcag aaaatcctca
    22441 acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc catttcatcg
    22501 ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt cgccgtccat ccatagcgtg
    22561 tactcatgcc ctgcccgctg ccaggaacgt tcgctacctt tcagtgcgtg cgctgcttgt
    22621 tccacttcat caagcaaggc caggttttct ttcacctctt cattaaacca gtgcccgacc
    22681 acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg cagaaattcg
    22741 taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcattg cgccattgat
    22801 gtaattatcg cagccttacc ggagaaaaac agcggaacgg cgatggttta gaaataaaaa
    22861 atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgctgact
    22921 ttttaaactg cagtctggaa aatcacacca tcggcttttt cagtgtactg agaaagctgg
    22981 ttgaagttca ggtaacggta agtatcaacg gcagttttat ccacctgcgc cacgtaggtc
    23041 tggtactctt ccggcgtcgg tagtttgcca atcagcgccg caacagcagc cagttccgca
    23101 gaagccagga agacattcgc gccagtcccc agacggttcg ggaagttacg ggtcgaggtg
    23161 gaaaccaccg tcgcgccgtc tgccacacgc gcctggttac ccatacacag ggaacagccc
    23221 gggatctcga tacgagcccc gctcttaccg aagacgctgt agtagccttc ttcagtcaac
    23281 tgtgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcaa ctggccttta
    23341 tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat gcaggaaccg
    23401 ataaacactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc gtcatccggg
    23461 tcgttcggag cacacaggat tggctcttta atatccgcca gatcgatgtc gatcactgcc
    23521 gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg
    23581 ccctgaatac gacgttccag ggtgcgacga tcgccgtaac cttccgcgat catccacttc
    23641 agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag cttgatggta
    23701 caaccggcag cagaacgctc ggcggacgca tcggtcagct caaaggcctg ctcaactttc
    23761 agatccggca gaccttcaat ttccaggatg cggccagaga agatgttttt cttgcctttc
    23821 ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcgtg taccagatcg
    23881 cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata
    23941 tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa
    24001 gagataccga tcgggaaacg ggtatgggag tcaccaccgg taccgacggt atccggcagc
    24061 agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga cacaccgcca
    24121 cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac
    24181 gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg
    24241 tctttcagtt catcacgggt catcgggcct gtggtgtcct gagagcctac agaagtcatt
    24301 ttcggttcac agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt
    24361 ttttgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca
    24421 tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg cccgataatc
    24481 agcggaatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag
    24541 gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca
    24601 atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc acccgcgtct
    24661 tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt
    24721 ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat
    24781 ttacgcgatg aacccgtacc cacaacgtca ccgacatacg ccagcgggaa acctttctgt
    24841 tgcagagctt cgatttgctt gatcgggcca acaacacctg gctggtctgg ctcaatacct
    24901 tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg tgaccacgca
    24961 tccggtgccg gagaaaggtc atcggtgtta gtttcgccag tgactttgaa gacggtaacg
    25021 gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc
    25081 attacctgct tcgcatattc gttgcctgct ttggctttct cttctacgtc atagaagtta
    25141 tcgaacatca gcagagtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca
    25201 tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg
    25261 atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttagcgat agcagccagg
    25321 aagcctgctt tgacataggc ggcttcatcg acgcctgggg gaacacggtt ggttaacaga
    25381 tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac aagtgcggcc
    25441 atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc
    25501 ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg
    25561 attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg
    25621 gcagaatagc aggattttga cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag
    25681 tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc
    25741 gcatagtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc
    25801 ctctttatac tcagatttac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc
    25861 ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt
    25921 gcagtgccgg gctttgtgcc gcctctgccg ggctctatat aaaaagccgc acagtggaag
    25981 cgcctgtgga accgctatcg acacaactgg ctgtgtctga cgctgccgcg gttacgcttc
    26041 ctgcaacggt ttccgcgcct cccgtaacac ccgccgtcgt taaatccgca ttcagcactg
    26101 cacaaatcga tcaatgggtt gcacctgtcg cgctataccc cgactctctg ctttcacaag
    26161 tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata
    26221 attcacttaa acaaggtgat gctgctattc aggcggtatc tgaccagccg tgggacgcta
    26281 gcgtcaaatc actggtggct tttccacaat tgatggcgtt gatgggcgaa aacccgcaat
    26341 gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac
    26401 aacgattgcg gcaactggcg caacaaaccg gttcgctgaa gtcatcaacc gaacagaaag
    26461 ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggtt cccgtcatac
    26521 catccaatac cgtttcaact gccaaccctg tcattacaga gcctgctaca accgtcattt
    26581 ccattgagcc tgccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt
    26641 acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac
    26701 cgtttgttga cagctttgta cgcggattcg gctatagcat gggcgttgct accacgtacg
    26761 cactattcag cagcatcgac tgggacgacg acgatcatga ccatcatcat catgacgatg
    26821 atgattatca tcaccacgat ggcgatcatc gtgacggtaa tggctggcaa cacaacggcg
    26881 acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat cttactgata
    26941 agaatatggc atggcggcac aatcaaaact accgtaatgg tgtgccctat catgatcagg
    27001 atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc acgcagctac
    27061 ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac
    27121 acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg
    27181 aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc caaccactga
    27241 cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc agcttctcct gagcagcgcc
    27301 aggcagttca cgagaaaatg cagactaacc cgcagaacca gcagcgaaga gaggcagcgc
    27361 gtgagcgcat tcagcccgcc tcgcctgagc agcgccaggc agtccgcgag aaaatacaga
    27421 ttaacccaca gatccagcag cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc
    27481 ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac
    27541 gtgataacgc ccgccagcgt gttcaatcag catcacctga acaacgtcag gtttttcggg
    27601 agaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact gccaggctga
    27661 ataacgagca acggtcagca gtacgcgaac gtctctctga gcgcggagca aggcgactgg
    27721 aaaggtaaat tacaggcgta aaaaaagcgg cgtggttagc cgctttttta attgccggat
    27781 gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt cggtaatgct
    27841 accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt gcgcgtggat
    27901 ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccaggccga tttcacccag
    27961 cagctcgccg ccgttagtac cgacaatcgc accaccgatc acacggtgag attctttgtc
    28021 gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac cagaagcagc
    28081 ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt tctcagtcag
    28141 acctacccat gcaacttctg gttcggtata ggcgatggac gggataactt tcggatcgaa
    28201 gtaatgtttt ttaccggcga taacttcagc ggcgacgtga ccttcgtgaa cacctttgtg
    28261 tgccagcatc ggctgaccga caatatcgcc gatagcaaag atgtgcggta cgttggtacg
    28321 cagctgtttg tcaacgcgga tgaaaccacg gtcgtcaact tccacgccag ctttgcctgc
    28381 gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt cgtaacgctg
    28441 cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt ctttcgcttc
    28501 aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac gcttggtgaa
    28561 gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt caaccacgtc
    28621 aatctgtgaa cccagcgcgt ggtaaacggt gcccatttcc agaccgatga taccgccacc
    28681 cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg agtcccagat
    28741 acgcggatct tcatgcggaa taaacggcag ttgaatcggg cgagaacccg ctgcaatgat
    28801 cgcgttgtcg aagttgatca cggttttacc gttctcacct tcaacttcca gggtgttagc
    28861 cccggtaaat ttacccagac cgttgaccac tttgactttg cggcctttcg ccataccagc
    28921 cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct tgtcgatatc
    28981 ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt cttcgataac
    29041 ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc aaacaccgcc
    29101 aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc aacggaaggc
    29161 agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga tttcagtact
    29221 catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc aggccgcagc
    29281 ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg aggattctgg
    29341 cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata ccctaaccac
    29401 caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt aaacaacaaa
    29461 cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt accagaaaaa
    29521 agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt cagacagcgt
    29581 gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa
    29641 ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc acaccggctc
    29701 catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa
    29761 gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct gcatttcgcc
    29821 cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc gagacagctc
    29881 gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt tcggggtatc
    29941 caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt cttccgacag
    30001 cgaactgttg aagcgaggca tctgctcaag cgctgcagca acggctttca tgatgaagac
    30061 aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct gctgtttacg
    30121 gaacgcttcc agctcggtga tatcggtttt gtcgaagtga gtaacatgcg ggatcatcac
    30181 ccagttacgg ctcaggttcg caccagaaat tttctggata cggcccagtt ccacttcttc
    30241 gatttcacca aacttgctga agtccacctt cggccacggc agcatgcctg ggataccgcc
    30301 gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt aagcctggac
    30361 atcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca ggttaacgcc
    30421 aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acgtaagcgt cgttttcagc
    30481 aaattcagat ttgccttccg ctttcgcagc cggtgctgct gccggggctt cagcttttgc
    30541 tgccggagcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc
    30601 aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt tgactttcag
    30661 ttccttcacg acgcctgcaa acggcgccgg aacttccata gaagctttgt cgccttctac
    30721 ggtgatcagt gactgttcag cggcaacttt gtcgcccact ttcaccatca cttcagtcac
    30781 ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag ctgctggtgt
    30841 aggggccgct gccggagccg cttcctgttt agccgccgga gctgccgcgc ctgcttcacc
    30901 cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca cgttcacttt
    30961 gatctctttc acggtgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc
    31021 tacggtgatc agcgactgtt cagcttcaac tttgtcgcca actttcacca ggatttcggt
    31081 cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg cggcagccgc
    31141 gggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc
    31201 tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt tgtcgccaac
    31261 agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca tagaggcttt
    31321 gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca ctttgaccag
    31381 gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt cgatagccat
    31441 tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat gttgaatttg
    31501 gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag ttcgcccagc
    31561 gccgcaacca cgacataaga agcatcaact tcgaagtggt gacgcaggtt ctcacggctg
    31621 tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg tacgtaagta
    31681 cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg agcgtcgttc
    31741 atcacctgag cgatatacgg aacgcgcgga gtttccagcg gatgcagcat gttccagcgt
    31801 tcacaatcct gaccatcacg cgccagctcg gtgaaggagg tcacgctata aacgtcagaa
    31861 cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg caggatagaa
    31921 ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag tttgtagata
    31981 cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta gttttcgttc
    32041 agcgtggtga tgtagtagta aacgttctct tgtttttcac cgtacatacg ctccagaccg
    32101 tcatgcataa tgacagcaac ttcgtaagcg taagccgggt cgtaagagat acagttcgga
    32161 atagtcagcg actgaatgtg gctgtgacca tcttcgtgct gcagaccttc accgttcagg
    32221 gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc gccagccgcc
    32281 cagcacagat cgccgatacg ctggaagccg aacatcgagt aatagatgta gaacggaatc
    32341 atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca acctgcgccc
    32401 agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata gtaagcaacc
    32461 tgctcgcggt cctgcggggt gtactgctga ccgttcgggc tgtaaatacc aatctgacgg
    32521 aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac cagacgatct
    32581 ttgatcgact tgttcttcag catcacgttc agggcacgaa cgaaagcgat agtggtagag
    32641 atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc
    32701 ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg acgctgagcg
    32761 tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag tttttcgata
    32821 tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc
    32881 attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat gccgtaacct
    32941 ttaatggtat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat
    33001 gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat ctgctcgtca
    33061 gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg ttcacgaacg
    33121 tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc gttcatcagc
    33181 tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct accccacatc
    33241 actttgatca cgttccagcc agcaccttcg aagatgcctt ccagttcgtt gatgatcttg
    33301 ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac gaaaaccagg
    33361 ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg ttcgtccatt
    33421 tcaccgtcgc cgaggaacgc gtaaacggtt tgtttagagg tatctttcag gccacggtgt
    33481 tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggacccag acccatagat
    33541 acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga agagaggcca
    33601 ttgccgtgaa cttcctgacg gaagttatcc agctgctcct gagtcagacg accttccagg
    33661 aaagcacgtg cgtatacgcc cggggagatg tggccctgga agtaaaccag gtcgccgcca
    33721 tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat ggttgcggaa
    33781 gactggaagg acgccatgtg gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc
    33841 gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc cagattaccc
    33901 ggatactcag gttgttcttc aacggggatg gtgttgatgt agttgctgat accggtgcct
    33961 gcggctacgt tgacaccgcc tttgcgggct tcagcaagca gttggtcgat cagatactga
    34021 gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca gtcgcgagtt
    34081 tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt atctatctaa
    34141 taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct caataagaca
    34201 ggttctacgt ttagttgccg cgcttttata tgcgcttgat ttacaacatc ttctggataa
    34261 tttttaccag aaaaatcact aattctttcg ttgctccaga cgacgcagag aacgctcacg
    34321 gcggctctct tcacggcttc tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg
    34381 cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata tgcgggtgcg
    34441 gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga agttctggcg
    34501 gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa ccacattgtg
    34561 ggccgcttcg gtgacggcaa tctgatactg gagtacggcg tttgattccg cgtccagatc
    34621 gccagactgc tgcgccagtt ctatggcgtg gtggagttca cggatgcgtt ccttgtcttc
    34681 atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc
    34741 gagcaagtca tactgtgatt caggatggtc ggagagcagc tccaccagcg gatcgctgaa
    34801 gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc
    34861 cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt caaactgttt
    34921 tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa
    34981 aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt tgctgtaggc
    35041 catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat ttcatgtctg
    35101 tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt gaaatcatga
    35161 aactgtgcac attttaacaa cttgacatat ataacgtttc aaagttgtaa ctatgcacaa
    35221 atgtagactt tacgtaggaa agggattttt taacgattat taaactataa aaatccgaat
    35281 tgaaccgatt cacttaccaa ttttgtgatt tttaattcat ttaaaacgaa tttaaattca
    35341 ttctgcatat tgagaggggt tgaggctgag ctttacaaac ggtttctttt taagcaactc
    35401 atcttcaacc atgcataaag cgggtgcatt cgctgccgca taccattatt cttgatctga
    35461 cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa acaaattaat acaacaaacg
    35521 gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaatcac cgcacgaggt
    35581 ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa aaaccgccat
    35641 attcagctta tcgcgctggg tggcgcgata gggaccgggt tattcctggg tagcgcctcc
    35701 gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg ttttatcgcc
    35761 tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt
    35821 agccactttg cttataaata ctggggcagc tttgctggct tcgcttctgg ctggaactac
    35881 tgggtactgt acgttttagt tgccatggca gagctgactg ctgtgggtaa atacattcag
    35941 ttctggtatc cggaaatccc aacttgggtt tctgccgccg tgttctttgt ggtgattaac
    36001 gccatcaacc tgaccaacgt aaaagtgttt ggtgagatgg agttctggtt tgccattatc
    36061 aaagttattg cggtagtagc gatgatcatc ttcggcggct ggctgctgtt cagtggtaac
    36121 ggcggtccgc aggcaagcgt tagcaacctg tgggatcagg gcggtttcct gccgcacggc
    36181 ttcaccgggc tggtgatgat gatggcgatt atcatgttct cgttcggtgg tctggaactg
    36241 gtggggatca ccgcagcaga agctgataac ccggagcaaa gtatcccgaa agcaactaac
    36301 caggttatct accgcatcct gattttctat attggttcgt tagccgttct gctctcactg
    36361 atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt ccacgagtta
    36421 ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac tgactgcggc gctctccgtg
    36481 tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca acagggtaac
    36541 gcgccaaaag cgctggcgtc tgtcgataaa cgcggcgtac cggttaacac cattctggtg
    36601 tctgcgctgg ttacagcatt gtgcgtattg attaactatc ttgctccgga atccgcattt
    36661 ggcctgttaa tggcactggt ggtatccgca ctggtgatca actgggcgat gatcagtctg
    36721 gcgcatatga agttccgtcg cgccaagcag gaacaaggcg tggtaactcg cttccctgct
    36781 ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt actggtgatt
    36841 atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg gctgatcgtg
    36901 ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc gcattaatct
    36961 ctctacgccc tcacccgtac agggtgaggg caataatctt tacaccaacg ccccgtaaat
    37021 cgtcagtagc gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc
    37081 tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga actgcgccag
    37141 acgcgttaac acctgatact gcgaagtatg gaaatcaccc agcgaagcaa accaggccgg
    37201 taacgctttc tcaccatgac cgatcaaggc atataccaca cccgcaagac gaaccggcac
    37261 ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat gcggcgtctg
    37321 atatcgtgcc agccagtatt gccatgcacg caagaacgca taccccatca gcgtaacggg
    37381 tccccaggtt ccccccacaa tcagccagaa cagcggtgca agataaaaac gaaagttaat
    37441 ccacagcaat gcattttgca gctcacgcaa atactcacgt tcgtcgcagc ctgccgggac
    37501 gccgtgaatc atggtgagtt cgccagccat cgtggcacgg gcatggctat cattacgtga
    37561 agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa tacacagcaa
    37621 accaatcagc agccacacca gcagcgtggg aacgttgaac aatactccct gcaatgcgcg
    37681 taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga
    37741 aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc agtgctcgcc
    37801 caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc
    37861 tccttgctga ccagcgcacg aaaccgtgcc caatcaaatg caggaccggg atcggttttc
    37921 cgatccggcg caatatcaca atggcccgtc atgttattag cgatatccgg atagcaatca
    37981 atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata cgccagcgta
    38041 tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcgcg cccctgatac
    38101 tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa catactggac tatttcacca
    38161 tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc aaagaaagga
    38221 tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc gccaaactcg
    38281 cctggcggta ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg
    38341 caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg ttctaacaac
    38401 atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg tttctacctt
    38461 atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac cctgacaccc
    38521 gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc
    38581 tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca aaacttttac
    38641 cggaaaattc tcgctctcat gccacggtga tcacccgcga gaatggcgta ttttgcggca
    38701 aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc ataatctggc
    38761 atgtggatga cggcgatgtc atcaacgcca atcaaccctt gttcgaactt gaaggcccat
    38821 cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag
    38881 ttgccagtaa ggtacgccac tatgtcgaat tactggaagg caccaacacg cagttgttgg
    38941 atacgcgcaa aaccttaccc ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg
    39001 gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa aaccatatta
    39061 ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc ctggctgcac ccggatgcgc
    39121 cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa gcaggagccg
    39181 atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca
    39241 acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg cgcgaatttg
    39301 ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taagcacgta caggctctcg
    39361 acctttcaat gcgttttcgc tagttgctga cagatttatc cgctccatag tgggcggata
    39421 atcacccctt ccccaacttt cttcgtaatg cctcgcaaat ttcttgtctt tcgctgcaac
    39481 ggaataaaaa acctcggaag ctgctttccc gtttcttttt ttgcccctcg cttgtgctga
    39541 tggcgtttgc caaagtagcg ccaacccaat caaggagcga aacagatgga caagcaacgc
    39601 ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt aagcgccatt
    39661 ggtattcccg cttatcaaaa ctacctgcgc aaagctgcac tcaccgacat actacaaacc
    39721 tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtgg attagatacc
    39781 tgcgacggtg gcagcaatgg cattccctcg cctaccacca cccgctatgt ttcagccatg
    39841 agtgtggcaa aaggcgtggt gtcgctgacc gggcaagaaa gtctcaatgg gctaagcgtc
    39901 gtcatgacgc caggttggga taacgcaaac ggcgtcaccg gctgggcgcg caactgcaat
    39961 attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg tcttccgctt tgatgacgcc
    40021 aagtaaggag cggcaatgaa tattccacag ctcacggccc tgtgcctgcg ttatcaggga
    40081 gtcttgctgg atgccagcga agaagtggtt catgttgcgg tggtcgatgc cccctcacat
    40141 gagttgctgg acgcattgca tttcgctacc accaaacgta ttgagatcac ctgctggacg
    40201 cgccaacaaa tggaaggtca cgccagtcgc acacaacaga cattgcccgt agctgttcag
    40261 gagaagcatc agcccaaagc agagttgctg actcgaacgt tacaatctgc gctggaacaa
    40321 cgcgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg cttgcgtatc
    40381 gacggcgtat tgcatccttt accggatgtt tcaccggatg ccggagtcgc attaaccgcc
    40441 agattaaaag tgctgggaaa cctggatatt gcggaacatc gcctgccgca ggacgggcaa
    40501 ttcactgtcg aactggcagg aaacgccgtc tcatttcgta ttgcgacctt accatgtcgg
    40561 ggtggtgaaa agctggtatt aaggttgtta cagcaggtga gccaggcact ggatgtcaac
    40621 acgcttggaa tgcagccatt acaactggcg gactttgctc atgccttgca acaaccacag
    40681 ggactggtgc tggtaactgg ccctaccggc agcggcaaaa cggtcacgct ttatagtgcc
    40741 ctgcaaacgc tgaataccgc tgacattaat atttgtagcg tcgaagatcc ggttgagatc
    40801 cccatagccg gactaaacca gacgcaaatc catccacgtg ccggactcac ctttcagggc
    40861 gttttgcgtg cgttattgcg ccaggatcct gacgtcatca tgatcggaga gatccgcgat
    40921 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
    40981 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
    41041 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaactttgc
    41101 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
    41161 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgc
    41221 acggccttat ttgaagttct gcccataaca ccggtcatac gtcagcttat ttccgctaat
    41281 accgacgttg aatcgctgga aactcacgcc cgacaggcgg gtatgcgtac gctttttgaa
    41341 aacggctgcc tcgccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
    41401 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
    41461 tgcgcaagat gggatgctat gggcagagag ccgtacttta ctgcttatgg cactacagca
    41521 acagatggtt accccactaa gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
    41581 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
    41641 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
    41701 gcaatcgctg gcgcacgatc tcgaacaggg cattgccttt tccaatgcct tattaccctg
    41761 gtcagaggta tttccgccgc tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
    41821 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
    41881 caaagtgaaa tcagcgttgc gttatcccat catcatttta gcgatggcaa tcatggtggt
    41941 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atatataaga ccttcaacac
    42001 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatgggg
    42061 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
    42121 accgacctgg cttatcgtgc ggcagaaatt gctgttacac atcccgatta tgggctcact
    42181 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
    42241 cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
    42301 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
    42361 aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta gtgagaacgg gagaggcctc
    42421 cggctcgctg gatctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
    42481 gctggcggat aacctagcag ccttactgga accggcgttg ctgatcataa cgggaggaat
    42541 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
    42601 tgggatggga taacgctggc gtgttgctcc acaccagcgt tgggagatta caggttgttg
    42661 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
    42721 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
    42781 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
    42841 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
    42901 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
    42961 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg ctttcgcgac atcgcccggc
    43021 gtggtgcagc caccatcgct gacgatcatt ccgcccagac cgtgcgcagc atcggcacat
    43081 tcgattaccg cagaaagttg cggataaccg acgcctgttt tgacacgagt tgtacaaaca
    43141 gaacctgggc caatgccaac tttaacgata tcggcaccgg agaggataag ctcctcacac
    43201 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
    43261 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacgaag
    43321 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
    43381 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
    43441 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
    43501 gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
    43561 cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
    43621 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
    43681 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
    43741 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
    43801 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
    43861 gtgcgctcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
    43921 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
    43981 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
    44041 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
    44101 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
    44161 ctggcttaac gacctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
    44221 agcaacctcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
    44281 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
    44341 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
    44401 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
    44461 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
    44521 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
    44581 cgtacatggc tgcgcattga gtttttgact cagcaactca ccgttaattt acccatcgtt
    44641 gaccacactg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
    44701 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
    44761 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
    44821 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
    44881 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
    44941 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
    45001 acctggattg ccagcctgaa cccgctcacc caggcgctta ccatggtgct ggatttaatt
    45061 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
    45121 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
    45181 tccggtcata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
    45241 gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtaaaaat gtcagaaact
    45301 attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt
    45361 cggccatttt gctccaaacg ttgtcagttg atcgacctcg gagaatgggc tgctgaagaa
    45421 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agatccaaag
    45481 cagtgacatt tgtctgatgc cgcacgtagg cctgataaga cgcggacagc gtcgcatcag
    45541 gcatcgtgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca aaaacaaatt
    45601 tatcaatgag ttagaaatcc ctgtaggcct gataagacgc gccagcgtcg catcaggcat
    45661 cttgtgcaaa tgtcggatgc ggcgaaaacg ccttatctga catacacacg cttaagcttc
    45721 gcaattaccg gttcattagc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    45781 cacttaccgg gttgcccttc tttaccccac ggctcccctt cccagctttc gaccagccag
    45841 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    45901 tgttgtggga taatcccgac ttcttcctga agttcacgca ccaccgcctg ttccggcgtt
    45961 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    46021 gcgcgacgcg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    46081 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    46141 cagttagctt ttattgcagg cggccatggc actgcttgta ttttttacca gaaccgcacg
    46201 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    46261 ctgcagagtc gtcatcctga tggctaagct gctgcatttg cgctaaacgc tcggcttcca
    46321 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    46381 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    46441 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    46501 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    46561 cgcctttctc gaagtgacgc atcatctctg caccaaccac ttcttcttta cgctgataca
    46621 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tcgggttctt
    46681 tatccagcca ctcagcaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    46741 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    46801 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    46861 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    46921 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    46981 ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt ttacgcatca
    47041 ttccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    47101 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    47161 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgctgcc agtaccgcat
    47221 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    47281 gcgcggcaac ttctgcctgc cagctaccac cgagcacaat atctgtacca cgacccgcca
    47341 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    47401 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    47461 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    47521 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    47581 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    47641 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    47701 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    47761 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgacggc
    47821 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    47881 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    47941 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    48001 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    48061 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tggcggatca
    48121 ggtgtggaat aattttattc acgcgtttat acatttccga gctgtcttct gccggaccgg
    48181 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga atccacttcg tccaccagcg
    48241 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    48301 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    48361 ttgccggtgc tggcatgccc ggcaggttga taccgacagt cagaccaagg aattcaaaca
    48421 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacatgca
    48481 cgcctttacc ggttagtgcg ttcagataag caggcagcgt tgcggtcagg gttttacctt
    48541 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    48601 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgtaccacg gcgaaagctt
    48661 ccgggatcag attttccagc acttcgcctt tttccagacg tgcacgaaac tctgcggttt
    48721 tccctttcag ttcttcgtcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    48781 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    48841 ataatttgat tagcataata aaatctcaaa cgccccgcgt tgcggagtta ataaaatgaa
    48901 gtaaaggttt attgttgtta gctgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    48961 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    49021 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    49081 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagcgt ttgcggtgcc
    49141 attgcgaagg aaagatgacg gattaccgtg cgaatggcat gttgatgcca gtaatcaacg
    49201 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    49261 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    49321 gcgttgctga gcgcaggcaa acctaaactc gccgcaacca tccctaataa gagatgcggc
    49381 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    49441 tcccgatcgt attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    49501 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    49561 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    49621 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acggctgaag
    49681 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    49741 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    49801 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcaagca
    49861 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    49921 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    49981 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    50041 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    50101 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    50161 cttcgacacg aacagtctct ttgatgcgaa caaatttctt ggcgcagttc aactcgtcga
    50221 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    50281 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    50341 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    50401 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    50461 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    50521 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    50581 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    50641 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgcagg
    50701 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcacg
    50761 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    50821 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    50881 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    50941 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    51001 gtttcgaact catccagacg caggtcgaag cccgctgtga tgttaaccag cacgccgcgt
    51061 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    51121 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    51181 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    51241 gcgatacctt gcacagcgcc tttgagtaca tcgttcgctg cgccaaacgc atccagcagg
    51301 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    51361 acatgcttgg acagttcagt gatcccctgc tccgcgaatg ccatacgctt cttgccttca
    51421 aagttgaagg gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    51481 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    51541 atgtctgcac cttccagcgc cgcacgcagt gcatcgcgat cctcatcagc cgcattgcgg
    51601 ccaacttctg gattagcgcc agctcccagt cctttggtga taccgctacc gatttgaatc
    51661 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    51721 acaccttcaa tgcgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    51781 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    51841 cgatttgtgc ctgtcgcctg aggccgtaat cattgtcggc ctcataaaaa ttaaaactct
    51901 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    51961 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    52021 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    52081 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    52141 gcgccaccag ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    52201 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    52261 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    52321 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    52381 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    52441 acattgccag cataaggaat taccttagtg tggcgcaatg ccccaccggt ataaacggcg
    52501 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    52561 gtcaataccg aataacttga tgccagaccg gcaaatatca gttggtcaac tttcagccca
    52621 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    52681 acttttgcct gcatccggac gcccgaaagt cctaccggat tcttgatccc ttcctgatag
    52741 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    52801 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    52861 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    52921 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcacgttg tacgcacttg
    52981 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgcgacgg gcagctgccc
    53041 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    53101 gcggtaccaa tctccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    53161 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    53221 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    53281 cctgctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc atcgtatcgc
    53341 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    53401 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    53461 agccctgcaa cacttcattg gcgctgcctt ccgggccata cagcattgga agcacctgct
    53521 tgctggtgcg atctggcggc acgctgaagg tatttccttc cgcgtctacc atgtgttgat
    53581 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    53641 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    53701 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    53761 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    53821 gttgcgcatc ttccatccag cccaacacga cccagccgct caccaacact gtcgttaaaa
    53881 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    53941 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    54001 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    54061 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcgctg
    54121 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    54181 actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca gaaatactgt
    54241 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    54301 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    54361 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    54421 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    54481 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    54541 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    54601 ttgctgcgta gtttatccat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    54661 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    54721 gcgataaaca ctttctgaaa gcccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    54781 tcgacaggat acgcgtcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    54841 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    54901 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    54961 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    55021 cagcatctcg gctacctgcg ccggatccgg caccagaatg ggatctattt tcccacgtcc
    55081 acgaattgtg cgacacagcg aacggctgtc cgcacccgga attggcgctt cgccagccgg
    55141 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    55201 caggtcgcgt gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    55261 ccagcctgcg cgcgccgctt taatggtggc gtccacttcc gtcgggtggt ggccgtagtc
    55321 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    55381 gaaatcaaaa cggcgaccag tcccctggaa actctccagc gcacgcaaaa tagcctcgtc
    55441 gtcaatgccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    55501 acctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tggcgcagca gcgtaaagtg
    55561 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    55621 gtaagttgtg gtctgacgtc ccactcgcgg taacaattcg cggatcaccg gatcatcaac
    55681 acacatcacc gcacgaccgt aaaacggcag gttgtgcaga aaattaataa aagtctgttt
    55741 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    55801 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    55861 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    55921 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tggttgtcgt
    55981 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    56041 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttatc
    56101 ggcagaaatg gcactggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    56161 gttgaaataa atcgtcgctc ccagattcat taactgctgc gtgaccggat ttggcgctaa
    56221 atcggaacca ctgatctgat agccttcatt ggccagaact tcggcaatac cgcccatacc
    56281 ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    56341 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    56401 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    56461 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    56521 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctcg
    56581 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    56641 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcaccgtt
    56701 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    56761 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    56821 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    56881 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccagcaca
    56941 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggtagcgc caacacatcg
    57001 gtacgcaccg ggttacccac cacttccgca ttggggaaag cacctggaaa cgcctgcatc
    57061 actttggtgg caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    57121 tgcagtacaa ccggaatgcc taacgaccac gcggccagac caccaggacc tgatacgtag
    57181 ccgcccatac cgagcaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    57241 caggcattga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaga
    57301 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    57361 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagatg gtgcgcaacc
    57421 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    57481 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    57541 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    57601 tgatcagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    57661 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    57721 ggtcaatttc taatgcttta cggccaatcg acatcgcgcg aaaagcgacg aagaatacca
    57781 ttaaaagtgc cagcaccaca ccgacatacc ccagttcttc gccgataatg gcgaaaataa
    57841 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgagttacct aaaccttgcc
    57901 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    57961 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    58021 gtatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    58081 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    58141 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    58201 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    58261 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    58321 aacgcgatgc ccctttaacc gagctaccca ctaccaggac gatcatcagc aggatgatag
    58381 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    58441 tgatcgccag aatgaatgcc aggatcagat agacgccatc acgcttcgcg aagaagaacg
    58501 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    58561 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    58621 tatctttttc ccgcgacccc atcacccagc cctttagcgc cgtggagatc cagaccagga
    58681 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    58741 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    58801 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgag gagccagcaa
    58861 gcgcatcgcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    58921 ctgcgcgccg tcacgaccga aacaatacag atgtacgtta tcgccattca ggtaacgcgc
    58981 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    59041 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    59101 aatccaacgt acgccgttat gctccagcac aacttcaaag cgatgcggca gaccagtgaa
    59161 tgtggttaac gctttcaggc tgctggcacg cggtaaccct gcggcatctg ccagtgccag
    59221 cgccgccagc gcattggtgt agttatgctg cccggaaagt ttcatttctt tcacgttcag
    59281 cactttctcg cctttcaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    59341 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    59401 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    59461 acgatactgt tgtaaaccaa acggatagcg atccatatga tcttcagtca cgttcagaat
    59521 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    59581 cacgtatagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    59641 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    59701 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac agaacagctc
    59761 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    59821 tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat cattcagact
    59881 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt
    59941 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    60001 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    60061 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    60121 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    60181 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    60241 acgaacacgc cccccataat caccagcagg aattcctgac gaagcagtac ggcgataatg
    60301 cctaacgcac cacctaacgc cagcgaacct acatcgccca taaagacctg cgccggatag
    60361 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    60421 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    60481 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    60541 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    60601 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    60661 gttgcgggcg tgtctttgcc aacaaggtac agcgcgaagg cgacacccag cgcaatgacc
    60721 gacatccaga aatacttcca acgagcgatc aaccctttgg tgtctttacg cactactttg
    60781 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    60841 acgtacggat tggacgggta agcccacagc agtacggaga tcacaatcgc cgtcaggatc
    60901 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    60961 cgcaccacct gaccaaagga aagtttttgc aaatgagcaa tcatacgcgg gcccatccac
    61021 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    61081 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    61141 tctcctgtaa agcgcgtact acctcttcca tggcggcact acgtgaaccc ttaactaaaa
    61201 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    61261 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccacgc
    61321 ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc cacctgtaca tggcaggctt
    61381 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    61441 cagccagtac ctggactgct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    61501 gcagcaactg gttttctgcc agttggatgg ggaacagacg gcctggaaca gctttcagat
    61561 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    61621 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tccacgctac
    61681 cggttggggt ttgcagggta aattccgtac cgtgcgaagt cacatggata ttggtggcgg
    61741 tgaaatcgct gttggcggca ttaggtgaga aacgccacac tttgcgtgag ccaattacgc
    61801 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    61861 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    61921 gatgcgccgc tgccaggttg ttggccagcg cagcttccgg gcgagtcaga ctcacagtcc
    61981 aggcaatttc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    62041 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    62101 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    62161 aggatcccgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat gcagccagtt
    62221 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgata tccagcggac
    62281 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcatgagcat
    62341 cgaaacgttc gcctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    62401 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atgtcggtaa
    62461 gttggctaag ggttacgcta atcatgcaat cacccccagc agacgcgcca ccgtgacgcg
    62521 atcggagtag tccagacgct gattgccaac aatctggtaa tcttcatggc ctttgcccgc
    62581 gaccagcacc acatcgttct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    62641 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    62701 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    62761 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    62821 gacacaccac agcttgcccg cacagtgcag acgcgccgcc tgtaaggctt tttccagtgc
    62881 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    62941 catacgtccg caaaccggtt gcagacgcgc ggcggttttc aacagatcag ccaatggata
    63001 gccgagtgcc aacagtgtcg ccagcgcgag cagcaggttg ctgacgttaa aagcgcccat
    63061 cagatggctt tcaatttcgc catcgcccca acttgagcta aagcgaatcg tcgcaccgct
    63121 gtcgtgatag ttcacgtcga tcgcttttaa ccagcgtccg tgacagttcg gattgatatg
    63181 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    63241 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    63301 ggcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    63361 gaccgacgcc gcaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    63421 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    63481 gactgccgaa ccggttgtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    63541 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gcagctgggt
    63601 cgtcgtggtt ttgccgttgg tgcccgttac gcccacgaga cgtaaattgt cagagggttc
    63661 atggtaaaag cggcccgcca gtgcagataa acgctcattg agctggctga gatagatgac
    63721 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    63781 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    63841 acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc
    63901 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    63961 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    64021 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    64081 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    64141 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    64201 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    64261 ctttaatcgc cgccttcacg ccgccgccgc ccggtagcgc cacgctttcc atcatatgca
    64321 ccacggtgcg gacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    64381 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    64441 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    64501 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    64561 cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc atcgctaacg
    64621 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    64681 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    64741 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    64801 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct ttcggcgtgc
    64861 cactcagatt gttagggttg tatgatgggc tgttagccat cgccagcact tcaccggtat
    64921 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcattgttca
    64981 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgcag
    65041 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    65101 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    65161 cttgactatc gacgttagta aagccaatga ggtgagcagt cacttcgccg gatggataat
    65221 aacggcgaga ttcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    65281 ccatgtcagg gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    65341 tgcgggctga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    65401 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    65461 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    65521 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    65581 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    65641 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    65701 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    65761 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    65821 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    65881 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    65941 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    66001 atcgcaaaag atcgtcacca ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    66061 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    66121 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    66181 taacttgcct agtgctcgca gctgacggcc ccccagtttt ttgagctgct cttcagtcat
    66241 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    66301 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    66361 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    66421 cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    66481 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    66541 ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag gttttcaata cccaggcgat
    66601 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    66661 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    66721 cggtgaagag acaccaagat cgaggagaat gccgtcgatc ttgccgataa gatcgcgctc
    66781 ggcaacgtat tcgcccagcg cggaaaaagg tccgtggatg atggagaagc gcggatcatc
    66841 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    66901 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    66961 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    67021 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    67081 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    67141 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    67201 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    67261 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc gacagacgcg
    67321 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    67381 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    67441 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    67501 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    67561 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    67621 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgcagcaaa ttaacgttat
    67681 gaatagctgt aagaaaaaat ccctcatcgt catcaccgtt ttaaaatgac tcactatttt
    67741 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    67801 cggttaacag tccctgttgc gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    67861 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    67921 cgcgctgtcg atatcactta attattgtga atttcagttg gagagttaat taacgggcgg
    67981 gtttaaaagc at
//
LOCUS       DAZUGK010000003        66126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZUGK010000003 DAZUGK010000000
VERSION     DAZUGK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.003"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..567)
                     /gene="folK"
                     /locus_tag="ACHY5W_000126"
     CDS             complement(88..567)
                     /gene="folK"
                     /locus_tag="ACHY5W_000126"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFS4445100.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIW"
     gene            complement(564..1961)
                     /gene="pcnB"
                     /locus_tag="ACHY5W_000127"
     CDS             complement(564..1961)
                     /gene="pcnB"
                     /locus_tag="ACHY5W_000127"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFS4445101.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENSLKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2021..2917)
                     /gene="gluQRS"
                     /locus_tag="ACHY5W_000128"
     CDS             complement(2021..2917)
                     /gene="gluQRS"
                     /locus_tag="ACHY5W_000128"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFS4445102.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLYHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2984..3439)
                     /gene="dksA"
                     /locus_tag="ACHY5W_000129"
     CDS             complement(2984..3439)
                     /gene="dksA"
                     /locus_tag="ACHY5W_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFS4445103.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3617..4321)
                     /gene="sfsA"
                     /locus_tag="ACHY5W_000130"
     CDS             complement(3617..4321)
                     /gene="sfsA"
                     /locus_tag="ACHY5W_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4445104.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTFWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4336..4866)
                     /gene="thpR"
                     /locus_tag="ACHY5W_000131"
     CDS             complement(4336..4866)
                     /gene="thpR"
                     /locus_tag="ACHY5W_000131"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFS4445105.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSTEKEKALSLLAGRIRQPGFTFTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4940..7369
                     /gene="hrpB"
                     /locus_tag="ACHY5W_000132"
     CDS             4940..7369
                     /gene="hrpB"
                     /locus_tag="ACHY5W_000132"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFS4445106.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLAFALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQNDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRL
                     LKMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVVFSRNQPAWQQRSQQLLKSLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLAVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7553..10087
                     /gene="mrcB"
                     /locus_tag="ACHY5W_000133"
     CDS             7553..10087
                     /gene="mrcB"
                     /locus_tag="ACHY5W_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFS4445107.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTISSGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQLSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10307..12556
                     /gene="fhuA"
                     /locus_tag="ACHY5W_000134"
     CDS             10307..12556
                     /gene="fhuA"
                     /locus_tag="ACHY5W_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFS4445108.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSDRDD
                     KQFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            12607..13404
                     /gene="fhuC"
                     /locus_tag="ACHY5W_000135"
     CDS             12607..13404
                     /gene="fhuC"
                     /locus_tag="ACHY5W_000135"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFS4445109.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13404..14294
                     /gene="fhuD"
                     /locus_tag="ACHY5W_000136"
     CDS             13404..14294
                     /gene="fhuD"
                     /locus_tag="ACHY5W_000136"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFS4445110.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETYLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKEMDALLATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14291..16273
                     /gene="fhuB"
                     /locus_tag="ACHY5W_000137"
     CDS             14291..16273
                     /gene="fhuB"
                     /locus_tag="ACHY5W_000137"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFS4445111.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGLALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16308..17588)
                     /gene="hemL"
                     /locus_tag="ACHY5W_000138"
     CDS             complement(16308..17588)
                     /gene="hemL"
                     /locus_tag="ACHY5W_000138"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFS4445112.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17754..17801
                     /gene="yadX"
                     /locus_tag="ACHY5W_000139"
     CDS             17754..17801
                     /gene="yadX"
                     /locus_tag="ACHY5W_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFS4445113.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17813..19234
                     /gene="clcA"
                     /locus_tag="ACHY5W_000140"
     CDS             17813..19234
                     /gene="clcA"
                     /locus_tag="ACHY5W_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFS4445114.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLLGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19258..19323
                     /gene="yadW"
                     /locus_tag="ACHY5W_000141"
     CDS             19258..19323
                     /gene="yadW"
                     /locus_tag="ACHY5W_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFS4445115.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19316..19660
                     /gene="erpA"
                     /locus_tag="ACHY5W_000142"
     CDS             19316..19660
                     /gene="erpA"
                     /locus_tag="ACHY5W_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFS4445116.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19707..20330)
                     /gene="yadS"
                     /locus_tag="ACHY5W_000143"
     CDS             complement(19707..20330)
                     /gene="yadS"
                     /locus_tag="ACHY5W_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFS4445117.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20368..21168)
                     /gene="btuF"
                     /locus_tag="ACHY5W_000144"
     CDS             complement(20368..21168)
                     /gene="btuF"
                     /locus_tag="ACHY5W_000144"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFS4445118.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21161..21859)
                     /gene="mtnN"
                     /locus_tag="ACHY5W_000145"
     CDS             complement(21161..21859)
                     /gene="mtnN"
                     /locus_tag="ACHY5W_000145"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4445119.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21943..23460
                     /gene="dgt"
                     /locus_tag="ACHY5W_000146"
     CDS             21943..23460
                     /gene="dgt"
                     /locus_tag="ACHY5W_000146"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFS4445120.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23590..25014
                     /gene="degP"
                     /locus_tag="ACHY5W_000147"
     CDS             23590..25014
                     /gene="degP"
                     /locus_tag="ACHY5W_000147"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFS4445121.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25169..26326
                     /gene="cdaR"
                     /locus_tag="ACHY5W_000148"
     CDS             25169..26326
                     /gene="cdaR"
                     /locus_tag="ACHY5W_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFS4445122.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26415..26801)
                     /gene="yaeH"
                     /locus_tag="ACHY5W_000149"
     CDS             complement(26415..26801)
                     /gene="yaeH"
                     /locus_tag="ACHY5W_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFS4445123.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26963..27775)
                     /gene="yaeI"
                     /locus_tag="ACHY5W_000150"
     CDS             complement(26963..27775)
                     /gene="yaeI"
                     /locus_tag="ACHY5W_000150"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFS4445124.1"
                     /translation="MISRRCLLLAAAATLTAGSGFGYMRYWEPGWLELTHQRIAFFKD
                     KAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLIFSAFS
                     NVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGCLYGLRLNCRPEVTMLELV"
     gene            complement(27829..28653)
                     /gene="dapD"
                     /locus_tag="ACHY5W_000151"
     CDS             complement(27829..28653)
                     /gene="dapD"
                     /locus_tag="ACHY5W_000151"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFS4445125.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28684..31356)
                     /gene="glnD"
                     /locus_tag="ACHY5W_000152"
     CDS             complement(28684..31356)
                     /gene="glnD"
                     /locus_tag="ACHY5W_000152"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFS4445126.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31418..32212)
                     /gene="map"
                     /locus_tag="ACHY5W_000153"
     CDS             complement(31418..32212)
                     /gene="map"
                     /locus_tag="ACHY5W_000153"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4445127.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKQLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32580..33305
                     /gene="rpsB"
                     /locus_tag="ACHY5W_000154"
     CDS             32580..33305
                     /gene="rpsB"
                     /locus_tag="ACHY5W_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4445128.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33440..34291
                     /gene="tsf"
                     /locus_tag="ACHY5W_000155"
     CDS             33440..34291
                     /gene="tsf"
                     /locus_tag="ACHY5W_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4445129.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34438..35163
                     /gene="pyrH"
                     /locus_tag="ACHY5W_000156"
     CDS             34438..35163
                     /gene="pyrH"
                     /locus_tag="ACHY5W_000156"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4445130.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35455..36012
                     /gene="frr"
                     /locus_tag="ACHY5W_000157"
     CDS             35455..36012
                     /gene="frr"
                     /locus_tag="ACHY5W_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4445131.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36104..37300
                     /gene="ispC"
                     /locus_tag="ACHY5W_000158"
     CDS             36104..37300
                     /gene="ispC"
                     /locus_tag="ACHY5W_000158"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFS4445132.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEKGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37489..38247
                     /gene="ispU"
                     /locus_tag="ACHY5W_000159"
                     /gene_synonym="uppS"
     CDS             37489..38247
                     /gene="ispU"
                     /locus_tag="ACHY5W_000159"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFS4445133.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38260..39117
                     /gene="cdsA"
                     /locus_tag="ACHY5W_000160"
     CDS             38260..39117
                     /gene="cdsA"
                     /locus_tag="ACHY5W_000160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4445134.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39129..40481
                     /gene="rseP"
                     /locus_tag="ACHY5W_000161"
     CDS             39129..40481
                     /gene="rseP"
                     /locus_tag="ACHY5W_000161"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFS4445135.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40511..42943
                     /gene="bamA"
                     /locus_tag="ACHY5W_000162"
     CDS             40511..42943
                     /gene="bamA"
                     /locus_tag="ACHY5W_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFS4445136.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43065..43550
                     /gene="skp"
                     /locus_tag="ACHY5W_000163"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43065..43550
                     /gene="skp"
                     /locus_tag="ACHY5W_000163"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFS4445137.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43554..44579
                     /gene="lpxD"
                     /locus_tag="ACHY5W_000164"
     CDS             43554..44579
                     /gene="lpxD"
                     /locus_tag="ACHY5W_000164"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFS4445138.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44684..45139
                     /gene="fabZ"
                     /locus_tag="ACHY5W_000165"
     CDS             44684..45139
                     /gene="fabZ"
                     /locus_tag="ACHY5W_000165"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4445139.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45143..45931
                     /gene="lpxA"
                     /locus_tag="ACHY5W_000166"
     CDS             45143..45931
                     /gene="lpxA"
                     /locus_tag="ACHY5W_000166"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFS4445140.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45931..47079
                     /gene="lpxB"
                     /locus_tag="ACHY5W_000167"
     CDS             45931..47079
                     /gene="lpxB"
                     /locus_tag="ACHY5W_000167"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFS4445141.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHNTHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAEALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47076..47672
                     /gene="rnhB"
                     /locus_tag="ACHY5W_000168"
     CDS             47076..47672
                     /gene="rnhB"
                     /locus_tag="ACHY5W_000168"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4445142.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47709..51191
                     /gene="dnaE"
                     /locus_tag="ACHY5W_000169"
     CDS             47709..51191
                     /gene="dnaE"
                     /locus_tag="ACHY5W_000169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4445143.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51204..52163
                     /gene="accA"
                     /locus_tag="ACHY5W_000170"
     CDS             51204..52163
                     /gene="accA"
                     /locus_tag="ACHY5W_000170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4445144.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52262..54403
                     /gene="ldcC"
                     /locus_tag="ACHY5W_000171"
     CDS             52262..54403
                     /gene="ldcC"
                     /locus_tag="ACHY5W_000171"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFS4445145.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMWMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54460..54849
                     /gene="yaeR"
                     /locus_tag="ACHY5W_000172"
     CDS             54460..54849
                     /gene="yaeR"
                     /locus_tag="ACHY5W_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4445146.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGMRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54914..56209
                     /gene="tilS"
                     /locus_tag="ACHY5W_000173"
     CDS             54914..56209
                     /gene="tilS"
                     /locus_tag="ACHY5W_000173"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4445147.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLSRTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGISFVWQRNA"
     gene            complement(56262..56522)
                     /gene="rof"
                     /locus_tag="ACHY5W_000174"
     CDS             complement(56262..56522)
                     /gene="rof"
                     /locus_tag="ACHY5W_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFS4445148.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56509..56709)
                     /gene="yaeP"
                     /locus_tag="ACHY5W_000175"
     CDS             complement(56509..56709)
                     /gene="yaeP"
                     /locus_tag="ACHY5W_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFS4445149.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56875..57420
                     /gene="yaeQ"
                     /locus_tag="ACHY5W_000176"
     CDS             56875..57420
                     /gene="yaeQ"
                     /locus_tag="ACHY5W_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFS4445150.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57417..57839
                     /gene="arfB"
                     /locus_tag="ACHY5W_000177"
     CDS             57417..57839
                     /gene="arfB"
                     /locus_tag="ACHY5W_000177"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFS4445151.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57853..58563
                     /gene="nlpE"
                     /locus_tag="ACHY5W_000178"
                     /gene_synonym="cutF"
     CDS             57853..58563
                     /gene="nlpE"
                     /locus_tag="ACHY5W_000178"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFS4445152.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58763..59587)
                     /gene="yaeF"
                     /locus_tag="ACHY5W_000179"
     CDS             complement(58763..59587)
                     /gene="yaeF"
                     /locus_tag="ACHY5W_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFS4445153.1"
                     /translation="MDKPKAYYRLLLPSFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSSSSVSHVAIYLGDNN
                     VAEATGDGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(59640..61358)
                     /gene="proS"
                     /locus_tag="ACHY5W_000180"
     CDS             complement(59640..61358)
                     /gene="proS"
                     /locus_tag="ACHY5W_000180"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4445154.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61469..62176)
                     /gene="tsaA"
                     /locus_tag="ACHY5W_000181"
     CDS             complement(61469..62176)
                     /gene="tsaA"
                     /locus_tag="ACHY5W_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFS4445155.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62173..62577)
                     /gene="rcsF"
                     /locus_tag="ACHY5W_000182"
     CDS             complement(62173..62577)
                     /gene="rcsF"
                     /locus_tag="ACHY5W_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFS4445156.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62695..63510)
                     /gene="metQ"
                     /locus_tag="ACHY5W_000183"
     CDS             complement(62695..63510)
                     /gene="metQ"
                     /locus_tag="ACHY5W_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFS4445157.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63550..64203)
                     /gene="metI"
                     /locus_tag="ACHY5W_000184"
     CDS             complement(63550..64203)
                     /gene="metI"
                     /locus_tag="ACHY5W_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFS4445158.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64196..65227)
                     /gene="metN"
                     /locus_tag="ACHY5W_000185"
     CDS             complement(64196..65227)
                     /gene="metN"
                     /locus_tag="ACHY5W_000185"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFS4445159.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65415..65990
                     /gene="gmhB"
                     /locus_tag="ACHY5W_000186"
     CDS             65415..65990
                     /gene="gmhB"
                     /locus_tag="ACHY5W_000186"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFS4445160.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPTQ"
BASE COUNT        15590 a        16488 c        18229 g        15819 t
ORIGIN      
        1 aagataagaa aataattttt tcttggtgga tgaaatagct gtatcgtttt gtatttctct
       61 cgtcaagaaa ataaaataat acagctatta ccagatggca ggttgtagta ctgctagcga
      121 agtgagtatt tcacgcaacg tctccccatc aggaaacacc aactccggcg cgatttcaaa
      181 cagcggccac agcataaatc cacgattttt catatcgtag tgcggaacgg tcagtcgttc
      241 agtatttatc acttcattac caaacagcat gatgtcgaga tccagcgtgc gcggtcccca
      301 gcgttcagct ttacggacgc gaccttgttg taactcaata cgctgtgtgt gattgagtag
      361 ctcttcgggt gcaagagagg tttccagcgc cacggctgcg tttaagtaat cgggttgatc
      421 ttgcggcccc agcggtggag tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg
      481 gatatcgcct aatgctttca gggcagcatt gacctgctcc agcggagagg ccagattgct
      541 gcctatggca atatacgcca ctgtcatgcg gtaccctcac gacgtggtgc gcgtttgcgt
      601 ggacgacgag tacgacgacg cggtgacggt tcttcatcca gctcgttgag catccctttt
      661 tggtctggtg gcgcggaaac ctggaactca ccccaccatt tcaccagacg ctgcagttca
      721 gcgttacgct caacttcagc tcgcaaggcc aacaggtcat aagccgcacg gaacttagga
      781 tgctccagca gtttccatgc gcgtttaccc tgacgtcggg acatacgcaa ctgcaactgc
      841 cagatatcgc gggttaacgt cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg
      901 tccagcacgt cgttcatcgc cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcttgg
      961 gcgatcttct gtgccgtctc cagcagtggg taccagaaca tggcggcaaa caggaacgcc
     1021 gggttcacgc gcatatcgtt atggatacgc gtatcggtat tcttcagcac ctgttcaatg
     1081 atccgctcca tcgggctgtc gccattttcc gtgaagtaac gggtaatggt cgggaacagt
     1141 ggctggaaca gatgatattc acacagcagt ttataggttt cgtaaccgta gcccgcttgt
     1201 agcagtttaa gcgattcttc aaacaggcgt gccggtggga tatcgttcag cagggtagcg
     1261 aggcgaggga tcggttctgc ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa
     1321 cgtactgcgc gcagcatacg taccggatct tcacggtagc gcgtttccgg gttaccaatc
     1381 agacggataa caccgtcctt cagatccttc ataccgccaa cgtaatcacg gacggtaaaa
     1441 tccgctacgc tgtaatacag gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg
     1501 atggagccga aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg
     1561 cggtcgctga cgttaccttc gtggtgtcca cggaaggtcg caacttcgat aatctccggg
     1621 ccaaacatca catgagccag acggaaacgg cgacccacca ggcggcagtt acggaacagt
     1681 ttgcgcacct gctcaggcgt ggcgttagtg gttacgtcaa aatctttcgg ctttttgccg
     1741 agtaacaggt cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc tttattgagc
     1801 ctgtacatta ccttcaggga attttcactg atatctttgc gagaaatagc atgctgctca
     1861 cgcgggatca ccgtcacctg tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg
     1921 cttagcacct tgcggcaaaa attagcgact cgggtaaaaa tagtacacct cggtagtgtc
     1981 aaacatcatt caggacaaaa aaatagcggc taatcatagc tcagcatgac gcatttgaga
     2041 atgttgaatt tacaattgcc gactcaggca ctgcggtaag ccgccagttt ttgacggctg
     2101 actgaaggat ttgctcgacg ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa
     2161 attgaagtgc cgcgattagt accgggcgtg gatcaccttt cggcaacgca ggcgcatgat
     2221 tctgcttgga aagtttagcg ccttgtggat taagcgccag cggcagatga atgtaatctg
     2281 gcactttcca gccaaaaagc tggtacagcg agatttgccg cactgtcggt tcgatcaaat
     2341 ctgccccgcg gacaatttca ctcacgccct ggaagtgatc atccaccacc accgccaggt
     2401 tataggcaaa caaaccatca cggcgatgaa taataaaatc ttcccgtgcc agtttttcgt
     2461 cggcgtgaat aatgccgcgc agctggtcag taaactgggt gaccggatgc tgctggcgga
     2521 tacgcactgc ggcgttgtct ggtccatgat acaacacccg gcaatgaccg tcgtaaatac
     2581 cgccaatgct ttgaatacgc gcacgcgtgc aggtgcagta ataacttagt ccttgttcat
     2641 gtaaccaggc gagtgcttca cgataggcat cgtgacgttg cgattgccag agaacatcgc
     2701 cgtcccagtg cagaccgtaa tgttccagct ggcgcaggat agtttctgcg gcaccgggaa
     2761 cttcacgagg cgggtcgata tcttcaatgc gtaccagcca gcgaccgtgc cgggcgcgag
     2821 cctgcaaata gctgccaagc gcagcaatca gagaaccaaa atgaagctcg ccggaaggag
     2881 agggggcgaa gcggccaata tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg
     2941 ggagcatttc ccgcctgtgg taaacgtgat ggaacggctg taattagcca gccatctgtt
     3001 tttcgcgaat ttcagccagc gttttgcagt cgatgcacag atcggctgtc gggcgcgctt
     3061 ccagacggcg aataccaatt tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt
     3121 ccactttttt cagcgtcttc tcgatctttt tgatcagctt acgctcgcga tcgcggttac
     3181 gcagttcgag gctgaactct tcttcctggg ctgcacggtc taccgggtcc gggaagttgg
     3241 ctgcttcatc ctgcatatgt gtaacggtgc gatcgacttc atccctgagt tgattacgcc
     3301 atgcttccag aatacgacgg aagtgcgcca gctgggcttc attcatatac tcttcgcccg
     3361 gcttctcctg atatggttcc accccagcga tggcgagaat actcagggac gatgttttac
     3421 ggttttgccc ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg
     3481 gggaaaaatg aggccgctat aaatagcaga tgcttttccg gatagcaatt atctaaacgt
     3541 aacacttgac aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa
     3601 ccagttactt actttactac aatgtaaccg gcagtgattt tttaagagcc atgccttcag
     3661 cagaaatttc cgctttgtaa gccagaattt ctacccccct ctgttgagct tctgacaata
     3721 gttgcgcgta tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca
     3781 gcacggcgaa aaagataacc gcacgctggc cttcagccgc tacgctcatc aactcccgaa
     3841 ggtgtttctg acctcgttca gtgaccgcat cgggaaaata tccctgttcg ttctccgcta
     3901 acgtaaccga tttcacttca atatagcagt ctggacgcga atccgcctgc aacataaagt
     3961 caatacggct gcgttctgcg ccgtatttta cttcgctttt cagcgagcta tagcctgaca
     4021 gttctgaaat tgattcatta aggatagcct ctttcgtcaa cctgttagcc caaaacgtgt
     4081 tgacgcaaat aaatgcgccg ctctggcttt gagttaattc ccaggtgtgt gggtatttcc
     4141 gtttggtgtt gtctgaagtc gaataccaga cggtatcgcc aggcgttgca caaccggtca
     4201 tcgcacccgt attcgggcag tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg
     4261 ctaaaaaacg tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga gagaattcca
     4321 ttgcgacaat ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg
     4381 tccacgggca aacgaggagg cgtaaagggt gaactccgtc accgcatacg accagttaaa
     4441 acctggcggc gggattgtca ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg
     4501 aaacggacga ttgctttgaa aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc
     4561 cagctggatt aagccgcgtg gaggctgacg catccctaac cacaccacac gcgaacgcag
     4621 ccattgtccg gcgtcatcaa gcgtgaatgt gaaaccaggt tgacgaatcc gtccggctaa
     4681 aagagaaagc gccttctctt tctcggtgct cacttcgcct aaaaatgcca gagtcagatg
     4741 caaattatcg gcggcgaccg gacgtcccgc ctcaagtggg aagtgtgtgg cgcgccagtg
     4801 gataatctgt tcgcggattt ctgcaggtaa gtcgatagca aagaacagac gttgcggttc
     4861 agacatgtga ggcactcggt tatgaattac cgcaatgcta caatgtggcg cgaagaatgt
     4921 taaccctctg gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact
     4981 taccgctctc gattgtgcgc cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc
     5041 aacctggctg ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa ttatcctgct
     5101 ggagccgcgt cgtctggcgg cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga
     5161 aaagccaggc gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac
     5221 ccgcctggaa gtagttaccg aaggcgtgct gacgcggatg atccagcgtg acccggaact
     5281 gagcggtgtt ggattggtga tccttgatga atttcatgag cgtagcttgc aggcggattt
     5341 ggcgtttgcg ctgttactcg atgtgcaaca aggtctgcgt gatgacctta aactgctgat
     5401 tatgtcggct acgctggaca acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt
     5461 catctcagaa gggcgctcgt ttccggttga acgccgttat ttaccgctgc ccacgcatca
     5521 gcgttttgac gatgccgttg cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc
     5581 attactgtta tttttacctg gcgtcggaga aattcagcgt gtgcaggaac aactggcttc
     5641 gcgcatcggc agtgatgtat tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca
     5701 gcgaaaagcg atcctcccgg caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat
     5761 tgctgaaacc agtttaacca tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg
     5821 tgtggcgcgt tttgatccgc gcacggggct tacgcgactg attactcaac gcgttagcca
     5881 ggcatccatg acgcagcgtg ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca
     5941 tttaatcgcc aaagaacaag cagaacgcgc tgccgcgcaa agtgaaccgg agatcttaca
     6001 aaatgatctt tccggtttgc tgatggaatt actgcaatgg ggatgcagcg atccggcgca
     6061 gatgagctgg ctggatcaac cgccagcagt gaatttactg gccgcgaaac gcctgttaaa
     6121 gatgctgggg gcgctggacg gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct
     6181 gggtaacgat ccgcgtttag cggcaatgct ggttagcgca aagagcgacg acgaagctgc
     6241 taccgcggca aaaattgccg ccattctcga agagccccca cggatgggca atagtgacct
     6301 gggcgtggtg ttttcgcgca atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa
     6361 aagcttaaac gtacgcggcg gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg
     6421 ggcttttgcc gatcgcattg ctcgccgccg tgggcaagat ggtcgctatc aactggcgaa
     6481 cggcatggga gcgatgctcg atgccgatga cgcgctaagc cgccacgaat ggttgatcgc
     6541 accgttatta ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt
     6601 cgatattgat gaattagtgc gacgctgtcc gcagctggtg cagcaatctg acaccgtgga
     6661 atgggatgac gcgcaaggta cactgaaagc ctggcgtcgg ctgcaaatcg gtcagttggc
     6721 ggtgaaagtg cagccactgg cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa
     6781 cggcatccgt gataaaggtt taagcgtgct caactggacg gcggaagcgg aacagctacg
     6841 cttgcgtttg ttatgcgccg gaaagtggtt gccagaatat gactggccag cggttgatga
     6901 tgaaagtttg ttggcgacgc tggaaacgtg gctgctgcca catatggccg gtgtacattc
     6961 actacgcggc ctgaaatcac tcgacattta tcaggcacta cgcggattac ttgattgggg
     7021 aatgcagcaa cgtctggata gtgaattgcc tgcgcattac actgtgccga cgggaagccg
     7081 gatcgccatt cgttatcatg aagataaccc gcccgcgctg gcggtgagaa tgcaggagat
     7141 gtttggtgag gccactaatc cgacgatcgc ccaggggcgc gtgccgctgg tgctggaatt
     7201 gctttcacct gcccaaaggc cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg
     7261 ggcgtatcgt gaggtgcaaa aagagatgaa agggcgttat cccaaacacg tctggccgga
     7321 cgacccggca aacacggcac cgacgcgacg gacgaaaaag tattcgtaag gttggttttc
     7381 tccctctccc tgtgggagag ggccgggggg agggcatcag cgcgcacgtt caccctcacc
     7441 ctcaagggag aggggaccga tcgagcacaa attttgagag atatcttctt ctgtcttgta
     7501 acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg
     7561 gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt
     7621 aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga
     7681 agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg
     7741 cggctggcta tggctgctgt taaaactggc tatcgttttt gccgtgctga tcgccattta
     7801 cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc
     7861 tgcggcagtt tatggtcgaa tggtcaacct tgagccagac atgaccatca gcaagaacga
     7921 gatggtgaag ctgctggagg caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg
     7981 cgaatttact gtgcaggcta acagcattga gatgatccgc cgtccgtttg attttccgga
     8041 cagcaaagaa gggcaggtgc gtgcgcgtct gacctttgat ggcgatcatc tggcgaccat
     8101 cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc gcctgatcac
     8161 catgatctcc tcaccaaacg gtgagcagcg tttgtttgtg ccgcgcagtg gtttcccgga
     8221 tttgctggtg gatactttgc tggcgacaga agaccgccat ttctacgagc atgatggaat
     8281 cagtctctac tccatcggac gtgcggtgct ggcaaacctg acagccggac gcacggtgca
     8341 gggggcgagt acgctgacgc aacagctggt gaaaaacctg ttcctctcca gcgagcgttc
     8401 ttactggcgt aaagcgaatg aagcctacat ggcgctgatc atggacgcgc gttacagcaa
     8461 agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg gcgacaacga
     8521 aatccgcggc ttcccgctgg cgagcttgta ttactttggt cgcccggtag aagaattgag
     8581 cctcgatcag caggcgctgt tagtcggtat ggtgaaagga gcgtccatct acaacccgtg
     8641 gcgtaacccg aaactggcgc tggagcgacg taatctggta ctgcgtctgc tgcaacagca
     8701 acagattatt gatcaagaac tctatgacat gttgagtgcg cgtccgctgg gtgttcagcc
     8761 gcgcggtggg gtgatctctc ctcagccagc ctttatgcaa ctggtgcgtc aggagcttca
     8821 ggctaaatta ggcgataagg taaaagatct ctccggcgtg aagatcttca ctacctttga
     8881 ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg cacttaagaa
     8941 acagcgtaag ctgagcgatc ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga
     9001 agttcgtgcg atggtcggag gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca
     9061 ggcgcggcgt tcgattggtt cccttgcaaa accagcgact tatctgacgg ctttaagcca
     9121 gccgaaaatc tatcgtctga atacgtggat tgcggatgcg ccaatcgcgt tacgtcagcc
     9181 gaatggccag gtctggtcac cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt
     9241 gatgctggtg gatgcgttga cccgttcgat gaacgtgccg acggtaaatc tggggatggc
     9301 gctggggctg cctgcggtta cggatacctg gatcaaactg ggcgtaccga aagatcagtt
     9361 gcatccggtt ccggcaatgc tgctgggggc gttgaactta acgccaatcg aagtggcgca
     9421 ggcattccag accatctcca gcggtggtaa ccgtgcaccg ctttctgcgc tgcgttcggt
     9481 aatcgcggaa gatggcaagg tgctgtatca gagcttcccg caggcagaac gcgctgttcc
     9541 ggcacaggcg gcgtatctga cactatggac catgcagcag gtggtacaac gtggtacggg
     9601 tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag ggactaccaa
     9661 caataacgta gatacctggt ttgcgggcat tgacggcagc acggtgacca tcacctgggt
     9721 cggccgtgat aacaaccagc cgaccaaact gtatggtgcc agcggggcaa tgtcgattta
     9781 tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc cagaagatat
     9841 tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt
     9901 gccggtctgg accagcgatc cgcaatcgct gtgccagcag agtgagatgc agcagcagct
     9961 gtcaggcaat ccgtttgatc agtcttcaca gccgcagcaa cagccgcaac agcaacctgc
    10021 tcagcaagag cagaaagaca gcgacggtgt agccggttgg atcaaggata tgtttggtag
    10081 taattaacgt ctaaacgtga cataccggat ggcgagttgc catccggtaa aataacatcc
    10141 catctaaaat attaaccctt tcttttcatc tggttgttta ttaacccttc aggaacgctc
    10201 agattgcgta ccgcttgcga acccgccagc gtttcgaata ttatcttatc tttataataa
    10261 tcattctcgt ttacgttatc attcacttta catcagagat ataccaatgg cgcgttccaa
    10321 aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca cagcggttag
    10381 cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta tcaccgttac
    10441 cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct gcaactattg cggcgcgaca
    10501 gtcagctacc ggcactaaaa ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt
    10561 tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta
    10621 cactcctggc gttgccgtgg gaactcgtgg cgcatccaac acttacgatt acctgatcat
    10681 ccgcggattc gccgctgacg gccaaagcca gaacaactat ctgaatggcc tgaagatgca
    10741 gggcaacttc tataacgatg cggttattga cccatatatg ctggagcgcg ctgaaatcat
    10801 gcgtggcccg gtttccgtgc tgtacgggaa aagcagccca ggtggcctgt tgaacatggt
    10861 cagcaagcgc ccgaccactg aaccgttgaa agaagttcag tttaaggccg ggactgacag
    10921 cctgttccag accggttttg acttcagcga tgcgctggat gatgacggcg tttactctta
    10981 tcgtctgacc ggtcttgcgc gctctgccaa tgcccagcaa aagggcgcgg aagagcagcg
    11041 ttatgccatt gcgccagcgt ttacctggcg tccggacgat aaaaccaatt tcaccttcct
    11101 ttcttacttc cagaacgagc cggaaaccgg ttattacggc tggttgccga aagagggaac
    11161 cgttgagccg ctgccgaatg gtaagcgtct gccgacagac tttaacgaag gggcgaagaa
    11221 caacacctat tctcgtaatg agaagatgat tggctatagc ttcgaccacg aatttaacga
    11281 cacctttacc gtacgtcaga accttcgctt tgcgcaaaat aaagtttcgc aaaagagcgt
    11341 atatggctac ggcatgtgtt cagatcctct ctattcctca aacccctctt cgagtccatg
    11401 tgccaacgtg ccgcagtctc aatggggcca tacactgact cgtcagtacg ttatcgataa
    11461 tgagaagtta gaaaacttct ctgttgatac tcagttgcaa agcaaattcg caaccggttc
    11521 agtggatcac actctgctga ctggcgttga ctttatgcgt atgcgtaatg acatcgactc
    11581 ctggttcgga tatgccggtt cagttgcacc atctgatatc tataatttag accgtagtga
    11641 ctttgatttt ggcgctcacc caaatccgtc tggtccatac cgcgttctgc tgaaacagaa
    11701 acaaaccggt ctgtatgttc aggatcaggc gcagtgggat aaagtgctgg ttacgcttgg
    11761 cggtcgctat gactgggccg atcagtcatc tttcaaccgt gattacggta ataaatccga
    11821 tcgcgatgat aaacagttca cctggcgtgg cggtgttaac tatctgttcg acaacggcgt
    11881 aacgccttat ttcagctaca gtgaatcgtt tgaacccgct tcgcagaccg atgcaaacgg
    11941 cgatctgttt gcaccttcga aaggcaaaca gtatgaagtc ggcgtgaaat atgtgccaga
    12001 agatcgccca attgtggtga ctggcgcact gtatcagctt accaaaacta ataacctgat
    12061 ggcggatccg aacggttcac tgttctcggt tgaaggcggt gagattcgtg cgcgtggcgt
    12121 agagctggaa gcgaaagcgg cactatcggc gagtgttaac gtagtcggtt cttacaccta
    12181 caccgacgcg gaatatacca ctgacaccaa ctacaaaggc aatacgcctg cacaggtgcc
    12241 aaaacacatg gcttcgctgt gggctgacta taccttcttt gacggtccgc tttcaggtct
    12301 gacgctgggc accggtggtc gttatactgg ctccagctat ggtgatccgg ctaactcctt
    12361 taaagtggga agttatacgg tcgtggatgc gttagtgcgt tatgatctgg cgcgagtcgg
    12421 catggctggc tccaacgtgg cgctgcatgt taacaacctg ttcgatcgtg aatacgtcgc
    12481 cagctgcttt aacacttatg gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc
    12541 aaccttccgt ttctaatttc tcttttgggg cacggatttc cgtgcccatt tcacaagttg
    12601 gctgttatgc aggaatacac gaatcattcc gataccactt ttgcactgcg taatatctcc
    12661 tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa
    12721 gtgaccggtc tgattggtca caacggttct ggtaaatcca ctctgctcaa aatgcttggc
    12781 cgtcatcagc ctccgtcaga aggggagatt cttcttgatg cccagccgct ggaaagctgg
    12841 agcagcaaag cgttcgcccg caaagtggct tatttgccgc agcagcttcc tccggcagaa
    12901 gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc cgtggcatgg cgcactaggg
    12961 cgctttggtg cggcagatcg cgaaaaagtt gaggaagcta tctcgctggt tggcttaaaa
    13021 ccgctggcgc atcggctggt cgatagtctc tctggcggtg agcgtcagcg ggcgtggatc
    13081 gccatgctgg tggcgcagga tagccgttgt ctgttgctcg acgaaccgac ctcggcgttg
    13141 gatatcgccc accaggttga tgtgctggcg ctggtgcacc gtttaagtca ggaacgtggc
    13201 ctgacggtca ttgccgtgct gcacgatatc aatatggcgg cacgctactg tgattatctg
    13261 gtcgccttgc gcggcggtga aatgattgct cagggaacgc ctgcggaaat tatgcgcggc
    13321 gataccctcg aaatgattta tggcatcccg atgggtattt tgccgcatcc ggcgggtgct
    13381 gcacctgtga gttttgttta ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt
    13441 taacggcgat ggcgctttct ccgttgttat ggcagatgaa taccgcccac gcggcggcta
    13501 ttgatcccaa tcgtattgtg gcgctggagt ggttgccggt ggaattactg ctggcgctcg
    13561 gcatcgtgcc ttatggcgtg gcggacacca tcaactatcg cctgtgggtt agcgaaccac
    13621 cgttacctga atcggtgatt gacgtcggtt tgcgcacaga acctaacctt gaactgctga
    13681 ccgaaatgaa accgtcgttt atggtctggt cggcaggata tggcccttca ccagaaatgc
    13741 tggcacgcat tgcgccgggg cgcggattta acttcagcga tggcaaacag ccgctggcga
    13801 tggcgcgtaa atcgctgacg gaaatggcag atttacttaa tctgcaaagc gcagccgaaa
    13861 cgtatttagc gcaatacgaa gactttatcc gcagcatgaa accccgcttt gtgaagcgtg
    13921 gtgcacgccc gttactgctg acgacgctta tcgatccgcg ccatatgttg gtcttcgggc
    13981 caaacagctt gttccaggaa attctcgatg agtacggcat cccaaatgcc tggcaagggg
    14041 aaaccaactt ctggggcagc accgccgtca gtatcgatcg tctggcggcg tataaagacg
    14101 ttgatgtgct gtgttttgat catgacaaca gcaaggagat ggacgcgcta ttggcaaccc
    14161 cgctgtggca ggccatgccg tttgtccgtg caggacgttt tcagcgcgtt ccggcggtct
    14221 ggttctatgg cgcgacgctt tcggcaatgc actttgtgcg tattctggat aacgccatcg
    14281 gaggtaaagc gtgagtaaac gaattgcgct tttcccggcg ttattgctgg cgctgctggt
    14341 gattgtcgct acggcgctga cctggatgaa cttttcgcag gcgctgccgc gtagccagtg
    14401 ggcgcaggcc gcctggtcgc cggatattaa cgtcatcgag cagatgattt ttcactacag
    14461 cttgttaccg cgtctggcga tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt
    14521 gctgtttcag caagtgctgc gtaacccgct ggcggaaccg acgacgttgg gcgttgctac
    14581 aggcgcgcaa ctggggatta ccgtcacgac gctctgggcg atccctggtg cgatggcgag
    14641 ccagtttgct gcgctggcag gggcttgtgt tgttggctta attgtctttg gcgtcgcgtg
    14701 ggggaaacgg ctgtcgccgg taacgctgat tctcgcgggg ttggtagtga gcctttattg
    14761 cggggcaatt aatcagctac tggttatctt ccatcatgac caactgcaaa gcatgtttct
    14821 gtggagcact ggaacgctga cgcaaaccga ctggggcggc gttgagcgtt tatggccgca
    14881 gctgctgggc ggcgtgatgc tgacgttatt gctacttcgc ccgttaaccc tgatggggct
    14941 tgatgatggc gtggcgcgca atctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct
    15001 gtcgctggcg atagtcatca gtgcgctgct ggtgaatgcg gtggggatta tcggctttat
    15061 cgggttgttc gcgccgttgc tggcaaaaat gctgggggcg cggcgtctgc tgccacggct
    15121 gatgctggcg tcgctgattg gggcgctgat cctctggctt tccgatcaaa tcattctctg
    15181 gctaacccgc gtgtggatgg aagtttctac cggttcggtc actgcgttga tcggtgcgcc
    15241 gctgctactg tggctgctgc cgcgtttacg cagcattagc gcgccggata tgaaggtcaa
    15301 cgatcgtgtc gcgactgaac gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct
    15361 gctgttgatg gctgtggtgg tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg
    15421 ggcgagcggg gcgttgctcg atgatttaat gccctggcgc tggccgcgaa ttatggcggc
    15481 gctatttgcg ggcgtcatgc tggcggtggc gggctgtatt attcagcgac tgaccggcaa
    15541 cccgatggca agcccggaag tgctggggat tagctccggc gcagcgtttg gcgtggtact
    15601 gatgctgttt ctggtaccgg gtaatgcttt tggctggctg ttacccgcgg gaagtatcgg
    15661 tgccgcggtg acgctgttga ttattatgat cgccgccggg cgcggtggat tttccccaca
    15721 ccgcatgtta ctggcgggga tggcgttaag taccgcattc accatgcttt taatgatgtt
    15781 gcaggcgagt ggtgacccgc gaatggcgca ggtgctcacc tggatctctg gttcgaccta
    15841 caacgccacc gatgcgcagg tctggcgcac cggaattgtg atggtgattt tgctggcgat
    15901 taccccgctg tgccgccgct ggctgaccat tttaccgctg ggtggtgata ccgcacgggc
    15961 cgtggggttg gcgttaacgc cgacgcgaat tgcgctgctg ctgttagcgg cctgtctgac
    16021 ggcgaccgcg acgatgacca ttggaccgtt gagttttgtt ggtttaatgg caccgcatat
    16081 cgcgcggatg atgggctttc gacggacgat gccgcacatc gtgatttcgg cgctggtggg
    16141 tggtttactg ctggtgttcg ctgactggtg tgggcggatg gtgctgtttc cgttccagat
    16201 cccggcgggg ctgctgtcga cctttatcgg cgcgccatat tttatctatt tgttgagaaa
    16261 gcagagccgt taataaaatg cctcgcccgg tgggcgaggc aaaatcatca caacttcgca
    16321 aacacccgac gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacagac
    16381 ataaagcccg cttcaaacgc tgacggtgcc aggtaaacac cttcgtccag catcatatgg
    16441 aagaaacgct tgaaacgctc gacgtcacag gccatcacat cctgatagca cgtcacggac
    16501 ccggcgtcgg taaagaagat accgaacata ccaccgacgt ggttaacgac cagcggaatt
    16561 cccgcttctt ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccagc
    16621 gtttcgtgaa cgcccggctg cgcgacttca ttcagacagg cgaaaccggc tgccatcgca
    16681 atcgggttac cggaaagcgt acccgcctga tagaccggac ccgtcggggc cagcgcatcc
    16741 attacatcac gacgaccacc gaatgcgcct accggcattc caccgccgat gatttttccg
    16801 aggcaggtta aatctggcac tacgccgtaa taatcctgtg cgccagctag cgctacgcgg
    16861 aaaccggtca tcacttcatc gatgatcagc aacgcgccaa attcgtcaca cagcgcacgc
    16921 agacctggca ggaactctgg cagcggcgga acacagttca tattgcctgc caccggctcg
    16981 acgataatac aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga
    17041 tcgttataag tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt
    17101 ggctggccta acgtgagtgc gccagaaccg gcttttacca gcaggcagtc agcgtgaccg
    17161 tggtaacaac cttcaaattt aataattttg tcgcgaccgg taaaaccacg ggccaggcgg
    17221 atggcgctca tggtcgcctc ggtgccggag ttcaccatgc gcaccatatc catggtcggg
    17281 accagttcgg tcaccagttg cgccattttc acttccattt cggttggtgc accaaagctt
    17341 aaaccacgct cggcggcttc aatcacggca ttgcggatcg ccggatggtt atggcccagc
    17401 accatcggcc cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga
    17461 taagcgccgt ccgctttttc gataaacagt ggagtgccgc ccacgccagt aaaggcgcga
    17521 acaggggagt tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac
    17581 ttactcatgg agggttcctg attcgtagaa aaagtgaatg gctgctattc tatgttattc
    17641 ataacaagtt aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct
    17701 tagagtataa aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac
    17761 gtgcgagtaa aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac
    17821 tgatactccc tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg
    17881 ccaacttctt gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg
    17941 cacgcttgtc gggctggctg cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca
    18001 acgtatgggg gcgctggtac atactgctga taattatccg ctactgttaa ccgtcgcttt
    18061 tctctgttcg gcggtgctgg cgatgttcgg ctacttttta gtgcgcaaat acgcgccgga
    18121 agcaggtggt tcggggatcc cggaaattga aggggcgctg gaagatcaac gtcccgttcg
    18181 ctggtggcgt gtattgccgg tgaaattctt tggcgggctg gggacactcg gcggaggcat
    18241 ggtgttgggg cgcgaagggc caaccgtgca gatcggcggc aatatcgggc ggatggtact
    18301 cgatgttttc cgcctgaaag gtgacgaagc gcgccatacg ctgctagcaa ccggtgctgc
    18361 tgcggggctg gcggcggcct ttaacgcgcc gctggcgggt attttgttta ttatcgaaga
    18421 gatgcgtccg cagtttcgct atacgttaat ttcgataaaa gcggtattta ttggcgtcat
    18481 tatgtcgacc attatgtacc ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa
    18541 actttctgat gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg
    18601 cattttcggc cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt
    18661 gcacggcggc aatattacca aatgggtact gttgggtggt gcgattggcg gtctgtgtgg
    18721 attgctgggg tttgtggcac cagcaacgtc gggcggcggt tttaacctga ttcctatcgc
    18781 taccgcgggg aatttcagca tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac
    18841 caccttactc tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct
    18901 gggtactgtg ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca
    18961 ccttgaggcg gggacgtttg ctattgccgg aatgggggcg ttactggcgg catctattcg
    19021 cgcgccgtta acggggatca ttctggttct ggagatgacc gataactacc agctcatttt
    19081 gccaatgatt attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc
    19141 gctatactcg gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg
    19201 aagcaaggcc gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg
    19261 gcgattatta ttgggttaga atttgcccaa ttgccgatgt cgtttggagc aaaatatgag
    19321 tgatgacgta gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct
    19381 gatcgctgac gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg
    19441 cagcggcttc cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat
    19501 cgaaaaacag ggcgttggcc tggtggttga tccgatgagc ttgcaatatc tggtcggcgg
    19561 ttccgttgac tataccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc
    19621 gaaaagcacc tgcggttgcg gttcttcctt tagcatctaa tctgttgttc atgtgccgga
    19681 taaactatcc ggcacatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt
    19741 agcttaagat gccaacgaat agccgccagc cgaatcaata gcgtcacgac catgcccatc
    19801 atactggctg tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg
    19861 ccgataatac aggcagttgc gtagatttct gtacgtaaaa tcatggggat ttcgcgggcc
    19921 agaacatcac gaatgatccc gccgccaacg ccagtaatga cgcccataca aaccgcgatt
    19981 aacggaccgg cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga
    20041 ccaacggcgt ccagcaccgg caacatccat tttggtaagc gtcttggctg gcgcaccagc
    20101 acgatggtca gcatgctggt taccattgca acgaccagat cggtgggatc tttcacccaa
    20161 aataccgggc cgtgatccag cgccatgtcg cgaattgtcc cgccgcctac agcggtaacc
    20221 acgcccagta ccagaacacc aaaagggtcc atacgcaatt ttccggctaa caaaacgccg
    20281 gagatggcaa ataccgctgt gccgactata tccagccaat agacgagcat tgttcaatcc
    20341 ccactgagca cctgaaaagg tcaggcacta atctacctgt gaaagcgcat tacagagctg
    20401 ttgtgcagcg aggataatac gtgggcttgc acgttcaaac cagtcactcg tgagaggaat
    20461 aacgggaatt ttgagctgtt caccccagta ttgtttgatt ttaggaattt ggtccggtcc
    20521 gcctgtaatg acaatcgcct gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg
    20581 ccagggaacc cggctgtctt taaagatgtt ttctccgcca caaacttcga gtacctggtt
    20641 ctgaatcgac tcttttccac tggtaaatgg cggattaatg ccgaattgca gaaaaacacg
    20701 ttttttaggt ttatcagcat attgcgcttt caattgcgcg tactgatcca gcagggattg
    20761 cgcggcttgt tcggccttat ccggttgcgg actccaaggg gccagttgac gtaacgcatt
    20821 ggcaatttgt tcaatgcttg tcgcatcgac ccacatcact tttattccca gcgaagccag
    20881 ctggtcaacc tgccgctcgg cattacctcc acgccaggca atcaccagat cgggtttcag
    20941 cgcgacaatg cgttccagat tcatcccctg ccaggtggaa acttgttcaa tcttttgcgc
    21001 ttgtggagga tagtcggaat agctgctgac cccaaccggc gtgatcccgg cggcaaaggc
    21061 aagttcagtg ttggcgggag aaagcgtgat gacgcgcggc gcggcgttga gccacagtgg
    21121 cgcaagaaaa gacagggcga ccagcgccct gaacagtgac ttagccatgt gccagtttct
    21181 gcaccagtga ctcaaccatc aggctggact gtttagcggc aacagccagg aactcatcga
    21241 agctaagatg agactgttga tcggccacgt cggagatggc gcgtactacg acaaacggga
    21301 cgttgaaatt gtggcagaca tgggcgattg ccgtcgcttc catctctaca gcaatggcct
    21361 gtgggaaatt gtggcggatt ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc
    21421 taacaatcag gccacgtaca gcgttaagat tcagttcggc aatgcaggcc tcagcggcag
    21481 cgatcagttt atcgtcagct ttaaagcctg ccggacagcc tggtaactga ccgtattcat
    21541 aaccaaatgc cgtgacatcc gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc
    21601 ccactttcag cgttggtgcc aggccaccgg cagaaccggt gttaataatc acatctggct
    21661 tgcagtgttc caacagcaaa gtggcaccca gcgccgcagc gactttaccg atgcccgatt
    21721 tcagaagcgc aacctcggtt ccattcagtt ggccggtata gatttcgcaa ccgccgagac
    21781 tgatagtttg acggttttcg attttgtcac gcagcagcgt aacttcttct tccattgcac
    21841 caatgatgcc gattttcata gatttactcg cgataagccc gatttgaagg catagtttac
    21901 catgcgctta cggggaagcg tatttctcac gcgggagagg acatggcaca gattgatttc
    21961 cgaaaaaaaa taaactggca tcgtcgttac cgttcaccgc agggtgttaa aaccgaacat
    22021 gagatcctgc ggatctttga gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt
    22081 cgtctgcaac aaaagaccca ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt
    22141 cttacccact cgatggaagt ccagcaggtg gggcgctaca tcgccaaaga aattttaagc
    22201 cgtctgaaag agcttaaatt gctggaagca tacggcctgg atgaactgac cggacctttt
    22261 gaaagcattg ttgagatgtc atgcctgatg cacgatatcg gcaatccgcc gtttggtcat
    22321 tttggcgaag cagcgataaa tgactggttt cgccagcgtt tatacccgga agatgccgaa
    22381 agccagcctc tgactgacga tcgctgcagc gtggcggcac tacgtttacg ggacggggaa
    22441 gaaccgctta acgagctgcg gcgcaagatt cgtcaggact tatgtcattt tgaggggaat
    22501 gcacaaggca ttcgtctggt gcatacattg atgaggatga atctcacttg ggcacaggtt
    22561 ggcggtattt taaaatatac ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat
    22621 cactatttaa tgaaaaagcc gggttattat ctttctgaag aagcctatat tgcccggttg
    22681 cgtaaagaac ttaatttggc gctttacagt cgttttccat taacgtggat tatggaagct
    22741 gccgacgaca tctcctattg tgtggcagac cttgaagatg cggtagagaa aagaatattt
    22801 accgttgagc agctttatca tcatttgcac gaagcgtggg gccagcatga gaaaggttcg
    22861 ctcttttcgc tagtggttga aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc
    22921 agtacggaag atcagttttt tatgtattta cgggtaaaca ccctaaataa actggtaccc
    22981 tacgcagcac aacgatttat tgataatctg cctgcgattt tcgccggaac gtttaatcat
    23041 gcattattgg aagatgccag cgaatgcagc gatcttctta agctatataa aaatgtcgct
    23101 gtaaaacatg tgtttagcca tccagatgtc gagcagcttg aattgcaggg ctatcgggtc
    23161 attagcggat tattagagat ttatcgtcct ttattaagcc tgtcgttatc agactttact
    23221 gaactggtag aaaaagaacg ggtgaaacgt ttccctattg aaacgcgctt attccacaaa
    23281 ctctcgacgc gccatcggct ggcctatgtc gaggctgtca gtaaattacc gtcagattct
    23341 cctgagtttc cgctatggga atattattac cgttgccgcc tgctgcagga ttatatcagc
    23401 ggtatgaccg acctctatgc gtgggatgaa taccgacgtc tgatggccgt agaacaataa
    23461 ccaggctttt gtaaagacga acaataaatt tttacctttt gcagaaactt tagttcggaa
    23521 cttcgcgcta taaaacgaat ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga
    23581 ctgaaataca tgaaaaaaac cacattagca ctgagtgcac tggctctgag tttaggtttg
    23641 gcgttatctc cgctctctgc aacggcggct gagacttctt cagcaacgac agcccagcag
    23701 atgccaagcc ttgcaccgat gctcgaaaag gtgatgcctt cagtggtcag cattaacgta
    23761 gaaggtagca caaccgttaa tacgccgcgt atgccgcgta atttccagca gttcttcggt
    23821 gatgattctc cgttctgcca ggaaggttct ccgttccaga gctctccgtt ctgccagggt
    23881 ggccagggcg gtaatggtgg cggccagcaa cagaaattca tggcgctggg ttccggcgtc
    23941 atcattgatg ccgataaagg ctatgtcgtc accaacaacc acgttgttga taacgcgacg
    24001 gtcattaaag ttcaactgag cgatggccgt aagttcgacg cgaagatggt tggcaaagat
    24061 ccgcgctctg atatcgcgct gatccaaatc cagaacccga aaaacctgac cgcaattaag
    24121 atggcggatt ctgatgcact gcgcgtgggt gattacaccg tagcgattgg taacccgttt
    24181 ggtttgggcg agacggtaac ttccgggatt gtctctgcgc tggggcgtag cggcctgaat
    24241 gccgaaaact acgaaaactt catccagacc gatgcagcga tcaaccgtgg taactccggt
    24301 ggtgcgctgg ttaacctgaa cggtgaactg atcggtatca acaccgcgat cctcgcaccg
    24361 gacggcggca acatcggtat cggttttgct atcccgagca acatggtgaa aaacctgacc
    24421 tcgcagatgg tggaatacgg ccaggtgaaa cgcggtgagc tgggtattat ggggactgag
    24481 ctgaactccg aactggcgaa agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc
    24541 caggttctgc ctaactcctc cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc
    24601 tcactgaacg gtaagccgat cagcagcttt gccgcactgc gtgctcaggt gggtactatg
    24661 ccggtgggca gcaaactgac cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac
    24721 ctggaactgc agcagagcag ccagaatcag gttgattcca gctccatctt caacggcatt
    24781 gaaggcgctg agatgagcaa caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa
    24841 acgggcactc cggctgcgca gatcggcctg aagaaaggtg atgtgattat tggcgcgaac
    24901 cagcaggcag tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg
    24961 ctggcactga acattcagcg cggcgacagc accatctacc tgttaatgca gtaatctccc
    25021 tcaacccctt cctgaaaacg ggaaggggtt ctccttacaa tctgtgaact tcaccacaac
    25081 tccatacatc ttcatcatcc tttaggcatt tgcacaatgc cgtacgttac gtacttcctt
    25141 atgctaagcc gtgcataacg gaggacttat ggctggctgg catcttgata ccaaaatggc
    25201 gcaggatatc gtggcacgta ccatgcgcat catcgatacc aatatcaacg taatggatgc
    25261 ccgtgggcga attatcggca gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc
    25321 attgctggta ctttcacagg gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct
    25381 gcacggtgtg cggcagggga ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt
    25441 aattggcctg acaggtgaac cagagaatct gcgtaaatat ggcgaactgg tctgcatgac
    25501 ggctgaaatg atgctggaac agtcgcggtt gatgcacttg ttggcgcagg atagccgttt
    25561 gcgggaggaa ctggtgatga acctgattca ggcagaggag aatactcccg cacttactga
    25621 atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt
    25681 cgacagcggt cagcttggtg tggacagcgc aatggcggag ttacaacaac tgcaaaacgc
    25741 gctgactacg cccgagcgta ataatctggt ggcgattgtt tcgctaaccg aaatggtggt
    25801 gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt
    25861 tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg tttcactggg
    25921 caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga aaacgacgat
    25981 ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg atctgatgtt
    26041 acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct
    26101 ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt
    26161 tcgtcacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc gtaataccct
    26221 ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca attttgatga
    26281 caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtaggtta tgcgttaagg
    26341 ttgaggcggc gctggctcat tcccctcagc ccgaagggta gaggggaatg cccgagttaa
    26401 ccgtcagttc gggattattt acgcgttagt ttttccagat cggcttcaat ctcgctgatc
    26461 ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg cttaagatca
    26521 acttcgctgc ggtcacgctg gcagatttga tcaagctcat caatgatata ccgtagattc
    26581 gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac gaccgtttta
    26641 cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt gtctttctgg
    26701 aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg atcaatttct
    26761 tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag
    26821 tcagtaaatt gtgcttattt tagcatttgg cctggcccgc ccggctcgac tgtgatcgca
    26881 gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta
    26941 ttgtggcgat aaaattcacg ttctacacca gttccagcat cgttacttcc gggcggcagt
    27001 tcagacgcaa accatacaaa cagcccacgc cacgggttgt gtagatgtgt ctttcgccaa
    27061 aggcatttaa tccggcgacg taacgtttat cttcgacagg ggcaaaaggt tcaccaacca
    27121 acggtacgcg cagttgcccg ccatgagtat ggccgcacag catcagatcc cagggttcat
    27181 cacgcatgac ttctttgctg tcgggattat gtgccagcac cagacgaggc agatttgctt
    27241 cgctggcggg aggtggtttg cattgtccgg cccataaatc accggtgcct accagttcga
    27301 attgtctgtt cggcgtggcg atcaccgtag cctggttaaa caacaccgtg atgcccactg
    27361 atttcaacgt ctcgccaatt aagtgatttt tttcagtacc aacagggcga tcgtggttgc
    27421 cgaagcaggc aaacgtcggc gcacattcgg caagggggga gagtacgtta ctaaacgccg
    27481 aaaaaatcag cggcatatca aataatacgt agtcgccacc cagcaatatc aaatcgggct
    27541 tttgttctat gccaagagca atcgcgtcag aaattaggct taaaggaaca aaacgagagt
    27601 aatggagatc tgccagaaaa agaattttga agggtgcggc tttgtcttta aaaaaggcga
    27661 tgcgctgatg ggttaattcg agccaacctg gttcccaata acgcatatag ccgaagcctg
    27721 atccggcagt taacgttgcg gcggcagcca gtaacaaaca tctgcgtgaa atcatcgctc
    27781 atccctgcaa aaaaatcggg cagcgtcgtg ctgcccgtgt gcatactttt agtcgatggt
    27841 acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat
    27901 aaccgcacag tagaggctgt atttgccatc tttcgacggc agattacctg aaacaaccac
    27961 agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt
    28021 gctctgacca atgtatacgc ccatggaaat gacggaacct tcttcgacaa tcaccccttc
    28081 aaccacttca gagcgcgcgc cgatgaagca gttatcttca ataatggtcg ggttagcctg
    28141 cagcggctcc agcacgccgc cgatacccac gccaccggaa aggtggacgt ttttaccaat
    28201 ctgcgcacag gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc
    28261 gccgatgttg acgtaagacg gcatcagcac ggtgttacgg gcaatgaacg cgccctgacg
    28321 taccgccgct ggtggcacaa cacggaagcc ttctttctgg aaacgtgctt cgtcgtaatc
    28381 agcgaatttc atcggcactt tatcgaagta gcggctttct gccccttcga tcacctgatt
    28441 atcattaata cggaaagaga gcagtaccgc ttttttcaac cactgatgcg tcacccactg
    28501 accgtcaatt ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt
    28561 taccgcttcg cgggtaacgg tgtctgcatt ggctggcgtg atctcggcac ggcgttcaaa
    28621 agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac
    28681 acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg
    28741 caactcgtta ttaagtgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg
    28801 ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt
    28861 cccgactctc gccagcagtc ctggctggtc gagggcgatc agttcgagga acgatttgcg
    28921 gtcggtatgg gtcggcaaaa acgtcacttc ggtttcaaca gtaaaatggc gtaatttggc
    28981 gggttggcga cggggctgcg gtggctgcca gctactttgc gtcagtactt gctccagacc
    29041 aaaccgaata acctcatgac gatctgcgga tagcgggctg ccatcgggtt ccagcacgat
    29101 gaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt ggacacttaa
    29161 attgcggcgg tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca
    29221 gataaaaatc tcggtgcctc cacgcgtagc ctgcggacta agcaatacca gcggtttgct
    29281 taaatcatgc tgtaataaat ggcgcgcatg ccaggccagt tgatttgggc tatggcggac
    29341 aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc
    29401 catgcgcagt agtgccagcg cctggagttg gtgatggcga acccgttcgc gcatatccgg
    29461 cgtgttttgc attccgcgtc gtagctgttt ttcggtggca aaatagagtt cgcgcaacag
    29521 actttgcttc cagctattcc acagcgtttc gttggtggcg caaatgtcag ccaccgtcag
    29581 gcataccaga taacgaagtc ggttttctgt ttgcacttct tcggcaaact gcttgatgac
    29641 ttccgggtcc tgaatatcgc ggcgttgggc ggtcaccgac atcaacaggt gctggcgaac
    29701 cagccaggcg accagctgtg tttcgcgtga gttcagcccg tggagttcgg caaaatgcac
    29761 tacatcctga gcaccaagaa tggaatggtc gccgccgcgt cctttggcga tatcgtgaaa
    29821 cagcgcggcg atgaaaatca gctcagttga cggtaggcgc ggccagacgt ccacacacaa
    29881 cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat
    29941 agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg
    30001 cgaccattgc ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg
    30061 ccgcaccgct ccggggtgac gcagaatgct caaaaacagt tttcgtgctt ccggaatatt
    30121 acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta
    30181 aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg
    30241 ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc
    30301 atcgtcgatt ggacggggtt tttcgtcggc gggaagggcg aggatcgctt catcgaacag
    30361 ttgcagcagc atctggttga gttcactgac gcggcgtgtc acgcggaagt aatccttcat
    30421 catccgctcg accggttcgt taccttcgcc actgtaattc agacgctggg cgacgctaag
    30481 ctggcgatcg aataacaggc gattatcgta acggctgacg accagatgca gggcaaagcg
    30541 aatacgccac aatatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa
    30601 gccaaaccca accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg
    30661 cagagtgtgg atatcgcgca agccgccagg gctacttttg atgtctggtt caagattgta
    30721 gctggtgccg tggtaacgct gatggcgctg gttctgttct tcaactttcg ccgcgtagaa
    30781 cttgtcggaa ggccagaatc cttcgctgaa aatatgtttt tgcagttcaa gaaatagcgc
    30841 aacatcgcca attaataagc gggattcgat caaattggtg gcgacggtta aatccgataa
    30901 cccttccagc atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc
    30961 ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg
    31021 gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc
    31081 gacggcgacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg
    31141 caggagctgg tcgataaact cggtgcgcgc ctcaatcaac tgttctgcag agatcccatt
    31201 gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta tcccaccgac
    31261 ggttaattca tcacggggcc agacgcatgg attttgcggt tgaccgggca gggtggggag
    31321 agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat
    31381 tatcgcatta aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg
    31441 ccgggatggt gtcatccttg cgtagcgtca gaatttcgca gccgttatca gtcaccacaa
    31501 tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt
    31561 tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc
    31621 caggtttcag tacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat
    31681 ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt
    31741 cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc
    31801 gtagcgccag gtacaggctt tcttgcgtga tgcggcacag acgttcgccc atgatggtcg
    31861 gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga
    31921 catcaatgtt aacgatatcg ccatctttca gctgcttagc atcgtccggg attccgtggc
    31981 acaccacttc attaatagaa atgcaaacgg atttcggata accgtgatag ccgaggcagg
    32041 cagaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg
    32101 tgctgacgcc cggtttaaca tacggttcga tcatctccag cacttcggca gccagccggc
    32161 cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg
    32221 tccatcagcg tcggtgatac cgacaatata tatgtaagtg ctgtcaatgg tatcacaccc
    32281 gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata
    32341 gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt attggtgtcc atgacgtatt
    32401 tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg
    32461 acaatctctc tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg
    32521 ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag gttttaatca
    32581 tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc
    32641 gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca
    32701 tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg
    32761 cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga
    32821 aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc
    32881 tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt
    32941 ctcaggacgg tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc
    33001 tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc
    33061 tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacctgggta
    33121 ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc
    33181 cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg
    33241 tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg
    33301 agtaataagg cacgctcgta cgagccctta tagaccaggt agtacacgtt tggttagggg
    33361 gcctgcatat ggcccccttt ttcactttta tatctgtgcg gtttaatgcc gggcagatca
    33421 catctccgag gattttagaa tggctgaaat taccgcatcc ctggtaaaag agctgcgtga
    33481 gcgtactggc gcaggcatga tggattgcaa aaaagcactg actgaagcta acggcgacat
    33541 cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg
    33601 caacgttgct gctgacggcg tgatcaaaac caaaatcgac ggcaactacg gcatcattct
    33661 ggaagttaac tgccagactg acttcgttgc aaaagacgct ggtttccagg cgttcgcaga
    33721 caaagttctg gacgcagctg ttgctggcaa aatcactgac gttgaagttc tgaaagcaca
    33781 gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt gaaaacatca acattcgccg
    33841 cgttgctgcg ctggaaggcg acgttctggg ttcttatcag cacggtgcgc gtatcggcgt
    33901 tctggttgct gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc
    33961 tgcaagcaag ccagaattca tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga
    34021 ataccaggta cagctggata tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa
    34081 aatggttgaa ggccgcatga agaaattcac cggcgaagtt tctctgaccg gtcagccgtt
    34141 cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg ctgaagtgac
    34201 tggcttcatc cgcttcgaag tgggtgaagg catcgagaaa gttgagactg actttgcagc
    34261 agaagttgct gcgatgtcca agcagtctta attatcgaaa aggagccgcc tgagggcggc
    34321 ttctttttgt gcccatcttg taaattcagc taacccttgt ggggctgcgc tgaaaagcga
    34381 cgtacaatgt cgctagtatt aattcatttc aatcgttgac agtctcagga aagaaacatg
    34441 gctaccaatg caaaacccgt ctataaacgc attctgctta agttgagtgg cgaagctctg
    34501 cagggcactg aaggcttcgg tattgatgca agcatactgg atcgcatggc tcaggaaatc
    34561 aaagaactgg ttgaactggg tattcaggtt ggtgtggtga ttggtggggg taacctgttc
    34621 cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca catggggatg
    34681 ctggcgaccg taatgaacgg cctggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac
    34741 gctcgcctga tgtccgctat tccattgaat ggcgtgtgcg acagctacag ctgggcagaa
    34801 gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac aggtaacccg
    34861 ttctttacca ccgactcagc agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg
    34921 ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc cggcgaaaga tccaaccgca
    34981 accatgtacg agcaactgac ttacagcgaa gtgctggaaa aagagctgaa agtcatggac
    35041 ctggcggcct tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt caacatgaac
    35101 aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa
    35161 taattcccgt gatggataaa taagggtaag attccgcgta agtatcgcgg gggcgtaagt
    35221 ctggttataa ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc agcaaccgga
    35281 gcgtacaaaa gtacgtgagg atggcgagca ctgcccgggg ccaaaatggc aaataaaata
    35341 gcctaataat ccagacgatt acccgtaata tgtttaatca gggctatact tagcacactt
    35401 ccactgtgtg tgactgtctg gtctgactga gacaagtttt caaggattcg taacgtgatt
    35461 agcgatatca gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc gttcaaaacc
    35521 caaatcagca aaatacgcac gggtcgtgct tctcccagcc tgctggatgg cattgtcgtg
    35581 gaatattacg gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt agaagattcc
    35641 cgtactctga aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga aaaagcgatt
    35701 atggcgtccg atcttggcct gaacccgaac tctgcgggta gcgacatccg tgttccgctg
    35761 ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg tgaagcagaa
    35821 caagcgcgtg ttgcagtacg taacgtgcgt cgtgacgcga acgacaaagt gaaagcactg
    35881 ttgaaagata aagagatcag cgaagacgac gatcgccgtt ctcaggacga tgtacagaaa
    35941 ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga agcagaactg
    36001 atgcagttct gatttcttga acgacaaaaa cgccgctcag tagatccttg cggatcggct
    36061 ggcggcgttt tgctttttat tctgtctcaa ctctggatgt ttcatgaagc aactcaccat
    36121 tctgggctcg accggctcga ttggttgcag cacgctggac gtggtgcgcc ataatcccga
    36181 acacttccgc gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg tagaacagtg
    36241 cctggaattc tctccccgct atgccgtaat ggacgatgaa gcgagtgcga aacttcttaa
    36301 aacgatgcta cagcaacagg gtagccgcac cgaagtctta agtgggcaac aagccgcttg
    36361 cgatatggca gcgcttgagg atgttgatca ggtgatggca gccattgttg gcgctgctgg
    36421 gctgttacct acgcttgctg cgatccgcgc gggtaaaacc attttgctgg ccaataaaga
    36481 atcactggtt acctgcggac gtctgtttat ggacgccgta aagcagagca aagcgcaatt
    36541 gttaccggtc gatagcgaac ataacgccat ttttcagagt ttaccgcaac ctatccagca
    36601 taatctggga tacgctgacc ttgagcaaaa tggcgtggtg tccattttac ttaccgggtc
    36661 tggtggccct ttccgtgaga cgccattgcg cgatttggca acaatgacgc ccgatcaagc
    36721 ctgccgtcat ccgaactggt cgatgggacg taaaatttct gtcgattcgg ctaccatgat
    36781 gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt aacgccagcg ccagccagat
    36841 ggaagtgctg attcacccgc agtcagtgat tcactcaatg gtgcgctatc aggacggcag
    36901 tgttctggcg cagctggggg aaccggatat gcgtacgcca attgcccaca ccatggcatg
    36961 gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa gtgcgttgac
    37021 atttgccgca ccggattatg atcgttatcc atgcctgaaa ctggcgatgg aggcgttcga
    37081 aaaaggccag gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg ttgctgcttt
    37141 tcttgcgcaa caaatccgct ttacggatat cgctgcgttg aatttatccg tactggaaaa
    37201 aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg cgaacgcgcg
    37261 tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga ggataatccg gctacagaga
    37321 gtcgcgctat ttgttagcgt agggcttcag tgatatagtc tgcgccatct gatcgtaagt
    37381 agttggcttt ataaggtcag gtatgccgtg gttttacacg gctttttttt gtataggctt
    37441 cagtattcct gagtaccgta aaccctgtca gggaataaaa aacgcgtgat gttgtctgct
    37501 actcaaccac ttagcgaaaa attgccagcg catggctgcc gtcatgttgc gatcattatg
    37561 gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg gcataaagcc
    37621 ggggcaaaat ccgtccgccg ggctgtctct tttgcggcca acaacggtat tgaggcgtta
    37681 acgctgtatg cctttagtag tgaaaactgg aaccgaccag cgcaggaagt cagtgcgtta
    37741 atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg acataacgtg
    37801 cgtctgcgta ttattggcga taccagtcgc tttaactcgc gtttgcaaga acgtattcgt
    37861 aaatctgaag cgctaacagc cgggaatacc ggtctgacgc tgaatattgc ggcgaactac
    37921 ggtggacgtt gggatatagt ccagggagtc aggcaactgg ctgaaaaggt gcagcaagga
    37981 aacctgcaac cagatcagat agatgaagag atgctaaacc agcatgtctg tatgcatgaa
    38041 ctggcccctg tagatttagt aattaggact gggggggagc atcgcattag taactttttg
    38101 ctttggcaaa ttgcctatgc cgaactttac tttacagatg ttctctggcc cgatttcgat
    38161 gaacaagact ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg tttcggcggc
    38221 accgagcccg gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta tcgcctgata
    38281 tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc gccggtgggg
    38341 ttcgccattg taacgctggt ggtctgcatg ctggcagcgt gggaatgggg acagcttagc
    38401 ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt attgttggcg
    38461 ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc atcaaccgct ggttgaaatc
    38521 tcactttggg cttcgttggg ttggtggatt gtcgcgctat tgctggtgct gttttaccca
    38581 ggttccgcag caatctggcg taactctaaa acattgcgca ttatttttgg cgtgctaacc
    38641 attgttccct tcttctgggg catgctggcg ttacgggcct ggcactacga cgagaatcat
    38701 tacagtggcg caatatggct gctctatgtc atgatcctgg tatggggcgc tgactccggc
    38761 gcatatatgt ttggcaaatt gtttggtaaa cataagctgg caccgaaggt ttctccgggt
    38821 aaaacctggc aaggctttat cggtggactc gcgactgcag cggtaatctc atggggttat
    38881 ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac tcatttgctc tattgtcgca
    38941 gcgttagcct cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga agcaggaatt
    39001 aaggacagcg gtcatttaat tccaggacac ggtggtattt tagatcgtat tgatagcctg
    39061 acggctgcgg taccggtctt tgcttgcttg ttgttactgg tattcaggac gctttaacgg
    39121 aaggtaatat gctgagtttt ctctgggatt tggcttcgtt catcgttgca ctgggtgtac
    39181 ttatcaccgt gcatgaattt ggtcatttct gggttgcccg gcgttgtggt gttcgcgttg
    39241 agcgtttctc aatagggttt ggtaaggcgc tctggcggcg aactgataag ctcggcaccg
    39301 aatatgttat cgccctgatc ccgttgggcg gttatgtcaa aatgctggat gagcgcgcag
    39361 aaccggtcgt tccggaactc cgccaccatg ccttcaataa taaatctgtc ggccaacgag
    39421 cggcgattat tgccgcaggt ccggttgcaa acttcatttt tgctatcttt gcctactggc
    39481 tggtttttat tattggtgtg cctggcgtac gtccggtggt tggcgaaata gcagccaatt
    39541 cgatagctgc ggaagcacaa attgcaccag gtacggaact aaaagccgta gatggtatcg
    39601 aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga taaaattggc gatgaaagca
    39661 ccaccattac ggtagcgcca tttggcagcg accaacggcg ggatgtaaag cttgatttac
    39721 gtcactgggc gtttgagccc gataaagaag atccggtatc ttcgctgggg attcgtcctc
    39781 gtgggccgca aattgaacct gtactggaaa atgtgcagcc aaactcggcg gcaagcaagg
    39841 caggtttgca agcaggcgac aggatcgtta aagtcgatgg tcagccctta acgcagtggg
    39901 tgacctttgt gatgcttgtc cgggataacc cgggtaaatc cttagcgtta gaaatcgaaa
    39961 ggcaggggag tcccttgtct ttgacattaa tcccggagag taaaccgggt aatggtaaag
    40021 cgattggttt tgtcggtatt gagccgaaag tcattccttt gccagatgag tataaagttg
    40081 tacgccagta tgggccgttc aacgccatcg tcgaagccac ggacaaaacg tggcagctga
    40141 tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa ctgaacaacc
    40201 tcagtgggcc gatctctatc gccaaggggg ctgggatgac agcggaactc ggggtagttt
    40261 attacctgcc gtttcttgcg cttattagcg tgaacttagg gataattaac ctgtttccgt
    40321 tgcccgtact tgacgggggg catctgctgt tccttgcgat cgaaaagatc aagggcggac
    40381 cggtatccga gcgggttcaa gacttttgtt atcgcattgg ctcgattctg ctggtgctgt
    40441 taatggggct tgcacttttc aatgatttct ctcggttatg agagttagtt aggaagaacg
    40501 cataataacg atggcgatga aaaagttgct catagcgtcg ctgctgttta gcagcgccac
    40561 cgtatacggt gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc ttcagcgtgt
    40621 cgccgttggt gcggccctcc tcagtatgcc ggtgcgcaca ggcgacacgg ttaatgatga
    40681 agatatcagt aataccattc gcgctctgtt tgctaccggc aactttgagg atgttcgcgt
    40741 ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa cgtccgacca ttgccagcat
    40801 tactttctcc ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc tcgaggcttc
    40861 tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt gccgatatcg agaaaggtct
    40921 ggaagacttc tactacagcg tcggtaaata tagcgccagc gttaaagctg tcgtgacccc
    40981 gctgccgcgc aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt cagctgaaat
    41041 ccagcaaatt aacattgttg gtaaccatgc tttcaccacc gacgaactga tctctcattt
    41101 ccaactgcgt gacgaagtgc cgtggtggaa cgtggtaggc gatcgtaaat accagaaaca
    41161 gaaactggcg ggcgaccttg aaaccctgcg cagctactat ctggatcgcg gttatgcccg
    41221 tttcaacatc gactctaccc aggtcagtct gacgccagat aaaaaaggta tttacgtcac
    41281 ggtgaacatc accgaaggcg atcagtacaa gctttctggc gttgaagtga gcggcaacct
    41341 tgccgggcac tccgctgaaa ttgagcagct gactaagatc gagccgggtg agctgtataa
    41401 cggcaccaaa gtgaccaaga tggaagatga cattaaaaag cttctcggtc gctatggtta
    41461 tgcctatccg cgcgtacagt cgatgcccga aattaacgat gccgacaaaa ccgttaaatt
    41521 acgcgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt ttgaaggtaa
    41581 cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag gtgcatggct
    41641 gggaagcgat ctggtcgatc agggtaagga gcgtctgaat cgtctgggct tctttgaaac
    41701 tgtcgatacc gatacccaac gtgttccggg tagcccggac caggtagatg tagtctacaa
    41761 ggtgaaagaa cgtaacaccg gtagcttcaa cttcgggatt ggttacggta ctgaaagtgg
    41821 cgtgagcttc caggctggcg tgcaacagga taactggtta ggtacaggtt atgctgttgg
    41881 tatcaacggg accaaaaacg attaccagac ctatgctgaa ttgtcggtaa ccaacccgta
    41941 cttcactgta gatggtgtaa gcctcggtgg tcgtctcttc tataatgact tccaggcaga
    42001 tgacgccgac ctgtccgact ataccaacaa gagttatggt acagacgtga cgttgggctt
    42061 cccgatcaac gaatataact cgctgcgtgc aggtctgggt tatgtacata actccctgtc
    42121 caacatgcag cctcaggttg cgatgtggcg ttatctgtac tctatgggtg aacatccgag
    42181 cacctctgat caggataaca gcttcaaaac ggacgacttc acgttcaact atggttggac
    42241 ctataacaag cttgaccgtg gttacttccc gacagatggt tcacgtgtca acctgaccgg
    42301 taaagtgacc attcctggat cggataacga atactacaaa gtgacgttag acacggcgac
    42361 ttatgtgccg atcgatgacg atcacaaatg ggttgttctg gggcgtaccc gctggggtta
    42421 tggtgatggt ttaggcggca aagagatgcc gttctacgag aacttctatg ccggtggttc
    42481 cagcaccgtg cgtggcttcc agtccaatac cattggtccg aaagcagttt acttcccgca
    42541 tcaggccagt aattatgatc cggactatga ttacgaatgt gcgactcagg acggcgcgaa
    42601 agacctgtgt aaatcggatg atgctgtagg cggtaacgcc atggcggttg ccagcctcga
    42661 gttcatcacc ccgacgccgt ttattagcga taagtatgct aattcggttc gtacttcctt
    42721 cttctgggat atgggtaccg tttgggatac aaactgggat tccagccaat attctggata
    42781 tccggactat agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat tacaatggat
    42841 gtccccattg gggccgttgg tgttctccta cgcccagccg ttcaaaaagt acgatggaga
    42901 caaggcagaa cagttccagt ttaacatcgg taaaacctgg taagtgttct ccacaaagga
    42961 atgtagtggt agtgtagcga tgactttagg cgatcaatat aagatcgccg ggccacgcaa
    43021 agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa aagtggttat
    43081 tagctgcagg tctcggttta gcactggcaa catctgctca ggcggctgac aaaattgcaa
    43141 tcgtcaacat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt tctaacacgc
    43201 tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc gatctgcagg
    43261 ctaaaatgaa aaagctgcag tccatgaaag cgggcagcga tcgcactaag ctggaaaaag
    43321 acgtgatggc tcagcgccag acttttgctc agaaagccca ggcttttgag caggatcgcg
    43381 cacgtcgttc caacgaagaa cgcggcaaac tggttactcg tatccagact gctgtgaaat
    43441 ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt gcttacaaca
    43501 gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa gtaatgcctt
    43561 caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt gatggcgata
    43621 tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt acgttcatgg
    43681 ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt gtcatgaccc
    43741 aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc tacctgactt
    43801 acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac attgcaccca
    43861 gtgcggtgat cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc gctaacgcgg
    43921 tgattgaatc cggcgttgaa ctgggcgata acgtgattat cggtgccggt tgcttcgtag
    43981 gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc atttaccatg
    44041 agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc gcagacggct
    44101 ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt cgcgtaatta
    44161 ttggcgatcg cgtggagatc ggtgcctgca ctaccatcga tcgcggcgcg ctggatgaca
    44221 ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat aacgtcgtga
    44281 ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg aaaattggtc
    44341 gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata tgcgacaaag
    44401 tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc gtctattcct
    44461 caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg gtgatgaaca
    44521 ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa caagactaac
    44581 gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt gcggcaggcc
    44641 gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta ctaacactca
    44701 tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt tcttactggt
    44761 ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa atgtctctgt
    44821 caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg gtgtgctgat
    44881 tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag gaaaactgga
    44941 accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc gcccggtcgt
    45001 gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg gcctgacccg
    45061 ttttaaaggg gttgctctgg tcgacggtaa agtagtttgc gaagcaacga tgatgtgtgc
    45121 tcgtagccgg gaggcctgat acgtgattga taaatccgcc tttgtgcatc caaccgccat
    45181 tgtggaagag ggcgcgtcga ttggcgcgaa cgcacacatt ggtccttttt gtatcgttgg
    45241 accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg tgaatggtca
    45301 tactaaaatt ggccgcgata atgagattta tcagttcgcc tccatcggcg aagttaacca
    45361 ggatttgaaa tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta accgcattcg
    45421 cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga aggtgggcag
    45481 cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag gtaaccgctg
    45541 tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact tcgcgatcat
    45601 cggcggcatg accgcagtcc atcagttctg catcattggt gcgcatgtga tggttggcgg
    45661 ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta accacgccac
    45721 gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg aggcgattac
    45781 cgctatccgc aatgcgtata agctgattta tcgtagcggt aaaacgctcg atgaagtgaa
    45841 accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta ccgatttctt
    45901 tgcacgctca acgcgcggtc tgattcgtta atgactgaac agcgtccatt aacgattgcc
    45961 ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg cgctctgaaa
    46021 gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca ggctgaaggc
    46081 tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga agtgctcggt
    46141 cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg tgaactaaag
    46201 ccagatgttt ttgttggcat tgatgcgcct gacttcaata ttaccctcga aggtaacctt
    46261 aaaaagcagg gtatcaaaac cattcattat gtcagtccgt ccgtctgggc gtggcgacag
    46321 aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct gcctttcgaa
    46381 aaagcgtttt atgacaaata caacgtaccg tgccgcttta tcggtcatac catggctgat
    46441 gccatgccat tagatccaga taaaaatgcc gcccgtgatg tgctggggat cccgcacaat
    46501 acccactgtc tggcattgtt gccgggaagt cgtggcgcgg aagttgagat gcttagcgcc
    46561 gatttcctga aaacggccca gcttttgcgc cagacgtacc ctgatctgga gatcgtggtg
    46621 ccgctggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctga agtcgcgcca
    46681 gacctttcag ttcatttgct ggatgggatg ggccgtgagg cgatggtcgc cagcgatgcg
    46741 gcactactgg catcggggac ggcagccctg gagtgtatgc tggcgaaatg cccgatggtg
    46801 gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt gaaaactgat
    46861 tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagagtt attgcaggaa
    46921 gagtgtgagc cgcaaaaact ggctgaggcg ctgttaccgc tgctggcgaa cgggaaaacc
    46981 agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat
    47041 gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtt tatccgcaca
    47101 cgcacttggt tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc gcggtcgtca
    47161 ccgctgcggt gatcctcgac ccggcgcgcc cgattgccgg gctgaatgat tccaaaaagc
    47221 tgagcgaaaa acgtcgtctg gcgctctgcg aagagatcaa agagaaagcg ttgagctgga
    47281 gtctgggccg cgcggaacct cacgaaatcg acgaactgaa tattttacat gcgaccatgc
    47341 tggcgatgca gcgagccgtc gctgggctgc atattgcgcc ggaatatgtg ttgattgatg
    47401 gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggc gatagccgcg
    47461 taccggaaat cagtgccgcg tctatcctgg cgaaagtgac gcgcgacgcc gaaatggcgg
    47521 cgctggatat tgttttcccg caatatggtt ttgcccaaca caaagggtat ccaaccgctt
    47581 ttcatctgga aaaactggct gaacacggcg cgaccgaaca ccatcggcgc agctttgggc
    47641 ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa gtaaaccgga
    47701 atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac tactcgatga
    47761 tcgatggcct ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg ggtatgccag
    47821 cactggcgat caccgatttc accaaccttt gcggtctggt gaagttctac ggagcgggac
    47881 atggcgcagg gattaagcct atcgtcgggg cagattttaa cgtccagtgc gacctgctgg
    47941 gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat cagaatctga
    48001 cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg atcatcgatc
    48061 gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcggg cgcatgggcg
    48121 acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt gtcgcgtttt
    48181 atgaagaaca cttcccggat cgctattttc tcgagctgat ccgcaccggc aggccggatg
    48241 aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg cccgtcgtgg
    48301 cgactaacga tgtgcgcttt atcgacagca gcgactttga cgcacacgaa atccgcgtcg
    48361 cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat tcgccgcagc
    48421 aatatatgcg tagcgaagag gagatgtgcg agctgtttgc cgacatccca gaagcccttg
    48481 ccaacaccgt tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt gaatacttcc
    48541 tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag cgtgcaaaag
    48601 agggcctgga agagcgtctg gcctttttat tccctgatga ggaagaacgt gttaagcgcc
    48661 gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag atgggcttcc
    48721 cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac ggcgtaccgg
    48781 tagggccagg ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg aaaatcaccg
    48841 acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg gaacgtgtct
    48901 ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt atcgaacacg
    48961 tagcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt acgatggcgg
    49021 cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc tttgtcgatc
    49081 gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg tttgaagccg
    49141 agccgcagct gccggaaatc tacgaagcgg atgaagaagt taaggcgctg atcgacatgg
    49201 cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg gtggttatcg
    49261 cgccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc aaacatccgg
    49321 tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc gacttccttg
    49381 gtttgcgtac gctcaccatc atcaactggg cgctggagat gatcaacaag cggcgggcga
    49441 agaatggcga gccgccgctg gatatcgccg cgatcccgct ggatgacaag aaaagcttcg
    49501 acatgctgca acgctcggaa accacggcgg tattccagct tgaatcgcgc ggcatgaagg
    49561 acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgcactg gtggcactgt
    49621 tccgccctgg tccgttgcag tcagggatgg tggataactt tattgaccgt aaacatggtc
    49681 gcgaagaaat ctcctatccg gacgtacagt ggcagcatga aagcctgaaa ccggtactgg
    49741 agccaaccta cggcattatc ctgtatcagg aacaggtcat gcagattgcg caggtgcttt
    49801 ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc gatgggtaag aaaaagccgg
    49861 aagagatggc taagcagcgt tctgtatttg ctgaaggtgc agaaaagaac ggaatcaacg
    49921 ctgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgctggttac ggatttaaca
    49981 aatcgcactc tgcggcctat gctttggtgt catatcaaac gttatggctg aaagcgcact
    50041 atccggcgga gtttatggcg gcggtaatga ccgccgatat ggacaacacc gagaaggtgg
    50101 tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca gatataaact
    50161 ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt attggcgcga
    50221 tcaaaggggt aggtgaaggt ccgattgagg cgatcatcga agcccgtaat aaaggcggct
    50281 acttccgcga actgtttgat ctctgcgccc gaaccgacac caaaaagtta aaccggcggg
    50341 tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacac cgcgcggcgc
    50401 tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa gcggaagcta
    50461 tcggtcaggc cgatatgttc ggcgtgctgg ccgaagaacc ggaacaaatt gaacaatcct
    50521 acgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt gaaacgttag
    50581 gtctgtacct gacgggacac cctatcaacc agtatttaaa agagattgag cgttatgtcg
    50641 gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc acggctgcgg
    50701 ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc ggtatctgca
    50761 cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc ctggataaat
    50821 accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc agctttgatg
    50881 acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac gaagcccggg
    50941 aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat gaccagcttt
    51001 taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca gtacatctct
    51061 actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg cgtgtctctc
    51121 cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag gtggaactgg
    51181 agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa cagccgattg
    51241 cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat gagaaactgg
    51301 atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa ctgacgcgta
    51361 aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat ccacagcgtc
    51421 cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg gctggcgacc
    51481 gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat ggtcgtccgg
    51541 tgatgatcat tggccatcaa aaaggtcgtg aaaccaaaga aaaaattcgc cgtaactttg
    51601 gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg gctgaacgct
    51661 ttaagatgcc tatcatcacc tttatcgaca ccccgggggc ttatcctggc gtgggcgcag
    51721 aagagcgtgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct cgcctcggcg
    51781 taccggtagt ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg gcgattggcg
    51841 tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg ccggaaggtt
    51901 gtgcgtccat tctgtggaag agcgccgaca aagcgccgct ggcggctgaa gcgatgggta
    51961 tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc catcatcccg gaaccgctgg
    52021 gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa ctgctggcgg
    52081 atctggccga tctcgacgtg ttaagcactg aagatttaaa aaatcgtcgt tatcagcgcc
    52141 tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac ttcggtggcc
    52201 cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc aggaggaaca
    52261 catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg agcccatcaa
    52321 agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac aaaacagcgt
    52381 tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt ttgactggga
    52441 tgagtacagt ctcgatttat gtagcgatat caatcagctt aatgaatatc tcccgcttta
    52501 tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgt ggatggcgct
    52561 ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta tgcgtcagta
    52621 caccgacgaa tatcttgata acattacacc gccgttcacg aaagccttgt ttacctacgt
    52681 caaagagcgg aagtacacct tttgtacgcc gggtcatatg ggcggcaccg catatcaaaa
    52741 aagcccggtt ggctgtctgt tttatgattt tttcggcggg aatactctta aggctgatgt
    52801 ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac accgggccac acctggaagc
    52861 ggaagaatac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta ccaacggaac
    52921 atcgacgtcg aacaaaattg ttggtatgta cgccgcgcca tccggcagca cgctgttgat
    52981 cgaccgcaat tgtcataaat cgctggcgca tctgttgatg atgaacgatg tagtgccagt
    53041 ctggctgaaa ccgacgcgta atgcgttggg gattctgggt gggatcccgc gccgtgaatt
    53101 tactcgtgac agcattgaag agaaagtcgc tgccaccacg caggcacaat ggccggttca
    53161 tgcggtgatt accaactcca cctatgatgg cttgctctac aacaccgact ggatcaaaca
    53221 gacgctggat gtcccctcga tccacttcga ttccgcctgg gtgccgtaca cccattttca
    53281 tccgatctac cagggtaaaa gtggtatgag cggcgagcgt gttgcgggaa aagtgatctt
    53341 cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc tgatccacat
    53401 taaaggcgag tatgacgagg aggcgttcaa cgaagccttt atgatgcata ccaccacctc
    53461 gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc gtggtaatcc
    53521 gggcaaacgg ctgattaacc gttcagtgga gcgggcgctg cattttcgca aagaggtcca
    53581 gcggctgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga
    53641 tgaagccgaa tgctggcccg ttgcgcctgg cgaacagtgg cacggcttta acgatgcgga
    53701 tgccgatcat atgtttctcg atccggttaa agtcactatt ttgacaccgg ggatggacga
    53761 gcagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcaaaat tcctcgacga
    53821 acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct ttagtattgg
    53881 catcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta agcgctctta
    53941 cgatctcaac ctgcggatca aaaatatgct gcccgatctc tatgctgaag atcccgattt
    54001 ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca
    54061 cgatcttccc ggtttgatgt tgcgggcatt cgatactttg ccggagatga tcatgacgcc
    54121 acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact
    54181 ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat
    54241 gcctggagaa atgctgacca aagagagccg cacagtactc gattttctac tgatgctttg
    54301 ttccgtaggg caacattacc ccggttttga aacggatatt cacggtgcga aacaggacga
    54361 agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggcttc
    54421 cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt
    54481 caccatattg cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt
    54541 cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat
    54601 ttggcgctta atgggcaata tgtgattgag cttttctcat ttccgttccc gccggagcgc
    54661 cccagccgac cggaagcttg cggcatgcgt catctggctt ttagcgttga tgatatcgat
    54721 gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca
    54781 tacacgcaaa aacgcttcac gttctttaac gatccggacg ggctgccgtt ggaactgtat
    54841 gagcagtaag gcttgtcatc gcggcatttg cccggtaacg tgccgggcat tgctactgta
    54901 aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac gccagattct
    54961 ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg
    55021 gacggaaaat ccgggggtcg ctctgcgcgc tattcatgtg catcacggtt taagtgccaa
    55081 tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt
    55141 ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg cgcggcaggc
    55201 gcgttatcag gcatttgccc gcaccttgtt gcccggtgaa gtgctggtca ccgcgcaaca
    55261 tctcgacgat caatgtgaaa cttttctgct ggcgctaaaa cgcggtagcg gccctgccgg
    55321 gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc gcccgttgct
    55381 ttcccgcacg cggggggaac tggcgcagtg ggcattggcg catggtttac gctggattga
    55441 agacgaaagt aatcaggacg actcatacga tcgtaatttt ctgcgcctgc gcgtagtgcc
    55501 gttattgcag cagcgttggc cgcattttgc cgaaacaacg gcccgcagcg ccgcactttg
    55561 tgctgaacaa gagagcctgc tggatgaact gctggcagat gatttagcac actgtcaatc
    55621 gccgcagggg acgctgcaga ttgtgccaat gctggcgatg agtgatgccc gccgcgcggc
    55681 gattatccgc cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg acgcgttggt
    55741 gaggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt tacgtttggg
    55801 cgcgtttgaa atccgacgct atcagtcgca actgtggtgg attaaatccg tcaccgggca
    55861 aagcgaaaac attgtgccgt ggcagacgtg gcttcaaccg ctggaattac cggcggggct
    55921 gggaagtgta cagcttaatg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt
    55981 cagcgtgcgt ttcaaagcgc caggattgct gcatattgtc gggcgtaacg gcggacgtaa
    56041 gctaaagaaa atctggcaag agctgggcgt gccgccgtgg ctacgtgaca ccacgccact
    56101 gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc aagaaggtgt
    56161 ggctgaaggt gagaatggca taagttttgt ctggcagcga aacgcttagt ttagtgaagg
    56221 ccggatatga cgcattaagc gtcgcatccg gcgaaagtca atcaggactc gctcaccacc
    56281 accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag ctcacgggtt
    56341 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcgctggct
    56401 ttggcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    56461 gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac
    56521 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56581 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56641 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56701 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56761 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56821 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56881 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56941 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    57001 cgcttgctgg cgtggttgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    57061 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    57121 gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc cgcagaagtg
    57181 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    57241 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    57301 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgtgatctgg
    57361 ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca accttcatga
    57421 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    57481 cgcagggcgc gggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    57541 acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    57601 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57661 tgaaccgcga agcagctctg gcccggctgg tggctgtgat taaagaatta acaacagaac
    57721 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57781 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt gcgcagcggt cgggaataaa
    57841 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57901 actctgatgg gttgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggcagcc
    57961 gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgccga ttgcgaagga
    58021 atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    58081 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    58141 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    58201 ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta tacgctggaa
    58261 ccggcacaat ccagtttacc catgacgccg atgaccctgc ggggcatgta tttttatatg
    58321 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    58381 gcagagctgg agcgtggcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    58441 tcagtagaag gtcactttac gcttgaggct aatccggata ccggtgcgcc gactaaagta
    58501 ttggcacccg atacggcagg gaaattttac ccaaaccagg attgcagtag tttggggcag
    58561 taacccgtct tgagacagaa acaaacgcaa aacaggccag aaggatatat ttcaacattt
    58621 tgaatttgca cgttttttgt aggccggata aggcgtttac gccgcatccg gcaatggtgc
    58681 tcaacgcctg atgcgacgct ggcgcgtctt atcatgccta caacccccct cataccttaa
    58741 ggcggataag gcatttactt tatcaccggg tcaaaccaac aaagcgaccc gccttgatat
    58801 aaatccctgg cttcaggtgc ccaacatact gtaactgcgt ttctggctta aacgcagaga
    58861 catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat tgcgccagcg
    58921 taagcggatg cccggctttg gcaaatgcat cagtgacaaa ttccgaacaa aaccacgact
    58981 ttttgtctcc ttcgaccaca ctgcttaact gcgctttcgc caggccgctg acgcactgtt
    59041 ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc ataaagggaa
    59101 taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg gcgaacgcgg
    59161 tgatttctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa agcttatcgc
    59221 tatgcttcat cgctttttta agggaaacaa tctggacacc atcccctgtc gcttctgcaa
    59281 cgttattatc acccagatag attgcaacgt gactcacaga ggaagagctg aagacgcgga
    59341 ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt ttgagatcag
    59401 gtaccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg aatttgacag
    59461 cccatgtttt cgcctcggca tctactgctg tggcagagga gtctggctgg ctgatatcaa
    59521 cggtacaggc cgaaagtaaa agaaagctgg ggagaagcag gcggtagtac gcctttggtt
    59581 tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc tttctggcat
    59641 cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat taactgtttc
    59701 tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg gtcgcccagc
    59761 acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc cggacgctct
    59821 ttgcggtcat ccagcagcac ttcgatacct tgcgcgcgca gttcgctgta cagtttctca
    59881 gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc cacctggaac
    59941 ggcgcgatag cgtcaggcca tacgatgccg cgctcgtcgt agttctgctc aatcgccgca
    60001 gccactacgc gcgttacccc gataccgtag caacccatag tcaggatttg gttacggcca
    60061 tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag ctggaagatg
    60121 tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg gcttggatcg
    60181 cctgccacca cgttacggat atccgcaact tccggggtag cgacatcgcg atcccagttg
    60241 atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact catcgccgca
    60301 acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga acccggaccg
    60361 gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct ggcaacctgc
    60421 ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc
    60481 agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt tttctcaatc
    60541 ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt atcaaccagc
    60601 gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc tgccagttca
    60661 atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc
    60721 agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc ctgtacggcg
    60781 cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag
    60841 gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat caggaattcg
    60901 cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt ctggatctga
    60961 tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag gtcagtgata
    61021 acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc
    61081 agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc tggctgaacc
    61141 accggcatcg acacctcgat tgcaccggcg ttgttcatct cttcacgcac gatgttttcg
    61201 acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga ggccagcttg
    61261 cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc ggcaggtgtc
    61321 tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt ccagttggaa
    61381 ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta ccagtgcaaa
    61441 agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag acttcaaaac
    61501 ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca taggttttgc
    61561 ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact tcgcggataa
    61621 acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg acctctgcgg
    61681 taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca ctggcatcgg
    61741 gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta ccatcgacca
    61801 gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc tctttcagct
    61861 ctaccagcga cataccaatt gggttagggc gaaaagtgga acgcgtagcg aaaacaccca
    61921 ttctggcatt accgccaaga cgaggcggac gcacagtcgg acgccagccg ccttccatcg
    61981 tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg cgaacggcgt
    62041 cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt accagacctg
    62101 gctggcgcgg aacggcgaac ttttctttat agggcgagcg aataacgcct atttgctcga
    62161 actggaaact gctcatttcg ccgtaatgtt aagcgcagaa ccgatacata cagcctgacg
    62221 atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaatacag cattggcttt
    62281 cattttagag gcgttgattt gcatccgctt acgtgcggtt ggaatgctcg gcggagagtc
    62341 ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga acggtttgcc
    62401 gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg ctttcggttt
    62461 tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag gggatctgct
    62521 taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag cacgcatagt
    62581 atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa aaatgacaag
    62641 acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta aaaattacca
    62701 gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt cgtcagactg
    62761 ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac gcgttacgat
    62821 caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg ccggagtcag
    62881 gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg cgtcgtccag
    62941 agaacgcggc agttgcggtg cttccagctc aacaattttc agattttttg ggttctcaac
    63001 aacatcaaga acggtcggca gaaggccaac gccatctttc agtttgatca agcccacttt
    63061 ttgcagcagc agcagtgaac gaccaaggtt agttgggtcg tttggcacgg caacctgcga
    63121 accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa ttggataaac
    63181 aaaagtattg cctactgcga ccagcttgta gccacgatct ttcagttgct gatcaaggta
    63241 cggtttatgc tggaaggcgt tggcgtcgat atcacctttg ctcaatgctt cgtttggcag
    63301 aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt tcgcaacttt
    63361 ctgcgcgact tctgcaacct gctgttcggc accaacaatc acgccgactt taatgtggtt
    63421 tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca gggctcccac
    63481 tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt atttatgttg
    63541 tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga actgaattaa
    63601 ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt tgtagccgat
    63661 gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac cacccatcgc
    63721 ggaataaccg actagggtaa tcagggtgat agttgccgca ttcaccagac ccggcaacgc
    63781 ttccggtaac agcactttac ggacgatctg cattggcgtg gcacccattg cgcgggaagc
    63841 ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg caataaacgg
    63901 tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg taccgacaat
    63961 aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg aacggaaaat
    64021 gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt gccccggacg
    64081 cgtgacataa agcaggacgc caaccggcag accaatcaca aacccaaaaa atccggatac
    64141 gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca tcggctcaga
    64201 cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg gcggcttgcg
    64261 tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttcacg ccaccggcgt
    64321 aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg gtttcagaaa
    64381 gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggg acgcagtcag
    64441 tgaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga tgcagggttg
    64501 actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca cttaccgtgt
    64561 cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaatg cgcttcacaa
    64621 cgtccatttc gtgagtgatc aacagaatcg tcaaacccag acggcggttg atgtctttca
    64681 gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct tcatcacaca
    64741 gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt ttctgcccac
    64801 cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaactaat gacagcaatt
    64861 ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc ggcagagcca
    64921 cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc ataccaatct
    64981 ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg ccatcgacca
    65041 gcacactacc ctcggttggg cgctccagca ggtttacaca acgtataagc gtactcttac
    65101 ccgcgcctga ggcaccgata acgccataaa tttgcccagc tggcacatgc aggctgacgt
    65161 tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata ttcgaaagtt
    65221 ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa tgcctgaaac
    65281 gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc aatcaggttt
    65341 atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtcaca tgccttatta
    65401 aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt gatggcacca
    65461 ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc gacggtgtta
    65521 ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta accaaccagt
    65581 ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc gagtggatgg
    65641 actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc ccgcatcatc
    65701 cgcagggtag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca catccgggga
    65761 tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat atggtgggcg
    65821 ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa gtgctggtgc
    65881 gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg ttaaatagcc
    65941 tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc gacgcaatga ttaaaagatg
    66001 agcgattgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc cctataatgc
    66061 gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag
    66121 aaaaat
//
LOCUS       DAZUGK010000004        61911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZUGK010000004 DAZUGK010000000
VERSION     DAZUGK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.004"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(150..1625)
                     /gene="ampG"
                     /locus_tag="ACHY5W_000187"
     CDS             complement(150..1625)
                     /gene="ampG"
                     /locus_tag="ACHY5W_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFS4445161.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1669..2247)
                     /gene="yajG"
                     /locus_tag="ACHY5W_000188"
     CDS             complement(1669..2247)
                     /gene="yajG"
                     /locus_tag="ACHY5W_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4445162.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2552..2869
                     /gene="bolA"
                     /locus_tag="ACHY5W_000189"
     CDS             2552..2869
                     /gene="bolA"
                     /locus_tag="ACHY5W_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFS4445163.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3213..4511
                     /gene="tig"
                     /locus_tag="ACHY5W_000190"
     CDS             3213..4511
                     /gene="tig"
                     /locus_tag="ACHY5W_000190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4445164.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKVNPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4757..5380
                     /gene="clpP"
                     /locus_tag="ACHY5W_000191"
     CDS             4757..5380
                     /gene="clpP"
                     /locus_tag="ACHY5W_000191"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4445165.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5506..6780
                     /gene="clpX"
                     /locus_tag="ACHY5W_000192"
     CDS             5506..6780
                     /gene="clpX"
                     /locus_tag="ACHY5W_000192"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFS4445166.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6968..9322
                     /gene="lon"
                     /locus_tag="ACHY5W_000193"
     CDS             6968..9322
                     /gene="lon"
                     /locus_tag="ACHY5W_000193"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFS4445167.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9513..9803
                     /gene="hupB"
                     /locus_tag="ACHY5W_000194"
     CDS             9513..9803
                     /gene="hupB"
                     /locus_tag="ACHY5W_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFS4445168.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9995..11866
                     /gene="ppiD"
                     /locus_tag="ACHY5W_000195"
     CDS             9995..11866
                     /gene="ppiD"
                     /locus_tag="ACHY5W_000195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4445169.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12017..12388
                     /gene="ybaV"
                     /locus_tag="ACHY5W_000196"
     CDS             12017..12388
                     /gene="ybaV"
                     /locus_tag="ACHY5W_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4445170.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12494..12892
                     /gene="fadM"
                     /locus_tag="ACHY5W_000197"
     CDS             12494..12892
                     /gene="fadM"
                     /locus_tag="ACHY5W_000197"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFS4445171.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12944..13639)
                     /gene="queC"
                     /locus_tag="ACHY5W_000198"
     CDS             complement(12944..13639)
                     /gene="queC"
                     /locus_tag="ACHY5W_000198"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFS4445172.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13704..15404)
                     /gene="ybaE"
                     /locus_tag="ACHY5W_000199"
     CDS             complement(13704..15404)
                     /gene="ybaE"
                     /locus_tag="ACHY5W_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFS4445173.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTP
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15504..16322
                     /gene="cof"
                     /locus_tag="ACHY5W_000200"
     CDS             15504..16322
                     /gene="cof"
                     /locus_tag="ACHY5W_000200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFS4445174.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALAERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16475..16933
                     /gene="decR"
                     /locus_tag="ACHY5W_000201"
     CDS             16475..16933
                     /gene="decR"
                     /locus_tag="ACHY5W_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFS4445175.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16963..18735
                     /gene="mdlA"
                     /locus_tag="ACHY5W_000202"
     CDS             16963..18735
                     /gene="mdlA"
                     /locus_tag="ACHY5W_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS4445176.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGCGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18728..20509
                     /gene="mdlB"
                     /locus_tag="ACHY5W_000203"
     CDS             18728..20509
                     /gene="mdlB"
                     /locus_tag="ACHY5W_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS4445177.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20690..21028
                     /gene="glnK"
                     /locus_tag="ACHY5W_000204"
     CDS             20690..21028
                     /gene="glnK"
                     /locus_tag="ACHY5W_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4445178.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21058..22344
                     /gene="amtB"
                     /locus_tag="ACHY5W_000205"
     CDS             21058..22344
                     /gene="amtB"
                     /locus_tag="ACHY5W_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFS4445179.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVLATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVCSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22392..23252)
                     /gene="tesB"
                     /locus_tag="ACHY5W_000206"
     CDS             complement(22392..23252)
                     /gene="tesB"
                     /locus_tag="ACHY5W_000206"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFS4445180.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQITQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23470..24042
                     /gene="ybaY"
                     /locus_tag="ACHY5W_000207"
     CDS             23470..24042
                     /gene="ybaY"
                     /locus_tag="ACHY5W_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFS4445181.1"
                     /translation="MKLVHMASGVAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24073..24384)
                     /locus_tag="ACHY5W_000208"
     CDS             complement(24073..24384)
                     /locus_tag="ACHY5W_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFS4445182.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24547..24643
                     /gene="ffs"
                     /locus_tag="ACHY5W_000209"
     ncRNA           24547..24643
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHY5W_000209"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24763..25116
                     /gene="ybaA"
                     /locus_tag="ACHY5W_000210"
     CDS             24763..25116
                     /gene="ybaA"
                     /locus_tag="ACHY5W_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFS4445183.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25158..26708)
                     /gene="pdeB"
                     /locus_tag="ACHY5W_000211"
     CDS             complement(25158..26708)
                     /gene="pdeB"
                     /locus_tag="ACHY5W_000211"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFS4445184.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYRSWQIDAAMIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26872..27342)
                     /gene="ylaC"
                     /locus_tag="ACHY5W_000212"
     CDS             complement(26872..27342)
                     /gene="ylaC"
                     /locus_tag="ACHY5W_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFS4445185.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27458..28009)
                     /gene="maa"
                     /locus_tag="ACHY5W_000213"
     CDS             complement(27458..28009)
                     /gene="maa"
                     /locus_tag="ACHY5W_000213"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFS4445186.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28181..28399)
                     /gene="hha"
                     /locus_tag="ACHY5W_000214"
     CDS             complement(28181..28399)
                     /gene="hha"
                     /locus_tag="ACHY5W_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFS4445187.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28425..28799)
                     /gene="tomB"
                     /locus_tag="ACHY5W_000215"
     CDS             complement(28425..28799)
                     /gene="tomB"
                     /locus_tag="ACHY5W_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFS4445188.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29345..32494)
                     /gene="acrB"
                     /locus_tag="ACHY5W_000216"
     CDS             complement(29345..32494)
                     /gene="acrB"
                     /locus_tag="ACHY5W_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFS4445189.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32517..33710)
                     /gene="acrA"
                     /locus_tag="ACHY5W_000217"
     CDS             complement(32517..33710)
                     /gene="acrA"
                     /locus_tag="ACHY5W_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFS4445190.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33852..34499
                     /gene="acrR"
                     /locus_tag="ACHY5W_000218"
     CDS             33852..34499
                     /gene="acrR"
                     /locus_tag="ACHY5W_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFS4445191.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34627..37989
                     /gene="mscK"
                     /locus_tag="ACHY5W_000219"
     CDS             34627..37989
                     /gene="mscK"
                     /locus_tag="ACHY5W_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFS4445192.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPVKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNDSQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRIGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            38050..38148
                     /locus_tag="ACHY5W_000220"
                     /pseudo
     CDS             38050..38148
                     /locus_tag="ACHY5W_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38201..38362)
                     /gene="rsmS"
                     /locus_tag="ACHY5W_000221"
     CDS             complement(38201..38362)
                     /gene="rsmS"
                     /locus_tag="ACHY5W_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFS4445193.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38376..38903)
                     /gene="priC"
                     /locus_tag="ACHY5W_000222"
     CDS             complement(38376..38903)
                     /gene="priC"
                     /locus_tag="ACHY5W_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFS4445194.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38973..39350
                     /gene="ybaN"
                     /locus_tag="ACHY5W_000223"
     CDS             38973..39350
                     /gene="ybaN"
                     /locus_tag="ACHY5W_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFS4445195.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39503..40054
                     /gene="apt"
                     /locus_tag="ACHY5W_000224"
     CDS             39503..40054
                     /gene="apt"
                     /locus_tag="ACHY5W_000224"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4445196.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40183..42114
                     /gene="dnaX"
                     /locus_tag="ACHY5W_000225"
     CDS             40183..42114
                     /gene="dnaX"
                     /locus_tag="ACHY5W_000225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4445197.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41447..41511
                     /gene="dnaX"
                     /locus_tag="ACHY5W_000225"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42167..42496
                     /gene="ybaB"
                     /locus_tag="ACHY5W_000226"
     CDS             42167..42496
                     /gene="ybaB"
                     /locus_tag="ACHY5W_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4445198.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42496..43101
                     /gene="recR"
                     /locus_tag="ACHY5W_000227"
     CDS             42496..43101
                     /gene="recR"
                     /locus_tag="ACHY5W_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4445199.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43211..45085
                     /gene="htpG"
                     /locus_tag="ACHY5W_000228"
     CDS             43211..45085
                     /gene="htpG"
                     /locus_tag="ACHY5W_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFS4445200.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45266..45910
                     /gene="adk"
                     /locus_tag="ACHY5W_000229"
     CDS             45266..45910
                     /gene="adk"
                     /locus_tag="ACHY5W_000229"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4445201.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46042..47004
                     /gene="hemH"
                     /locus_tag="ACHY5W_000230"
     CDS             46042..47004
                     /gene="hemH"
                     /locus_tag="ACHY5W_000230"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFS4445202.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47001..47960)
                     /gene="aes"
                     /locus_tag="ACHY5W_000231"
     CDS             complement(47001..47960)
                     /gene="aes"
                     /locus_tag="ACHY5W_000231"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFS4445203.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48112..49416
                     /gene="gsk"
                     /locus_tag="ACHY5W_000232"
     CDS             48112..49416
                     /gene="gsk"
                     /locus_tag="ACHY5W_000232"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFS4445204.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASTLVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49546..51222)
                     /gene="ybaL"
                     /locus_tag="ACHY5W_000233"
     CDS             complement(49546..51222)
                     /gene="ybaL"
                     /locus_tag="ACHY5W_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFS4445205.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51460..52680)
                     /gene="fsr"
                     /locus_tag="ACHY5W_000234"
     CDS             complement(51460..52680)
                     /gene="fsr"
                     /locus_tag="ACHY5W_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFS4445206.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALADSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52898..54550
                     /gene="ushA"
                     /locus_tag="ACHY5W_000235"
     CDS             52898..54550
                     /gene="ushA"
                     /locus_tag="ACHY5W_000235"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS4445207.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARVLPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54587..55066)
                     /gene="ybaK"
                     /locus_tag="ACHY5W_000236"
     CDS             complement(54587..55066)
                     /gene="ybaK"
                     /locus_tag="ACHY5W_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFS4445208.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55270..56064)
                     /gene="ybaP"
                     /locus_tag="ACHY5W_000237"
     CDS             complement(55270..56064)
                     /gene="ybaP"
                     /locus_tag="ACHY5W_000237"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFS4445209.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPARLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QVMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56202..56543
                     /gene="ybaQ"
                     /locus_tag="ACHY5W_000238"
                     /pseudo
     CDS             56202..56543
                     /gene="ybaQ"
                     /locus_tag="ACHY5W_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(56758..59262)
                     /gene="copA"
                     /locus_tag="ACHY5W_000239"
     CDS             complement(56758..59262)
                     /gene="copA"
                     /locus_tag="ACHY5W_000239"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFS4445210.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDVSVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQSLLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59524..60456
                     /gene="glsA"
                     /locus_tag="ACHY5W_000240"
     CDS             59524..60456
                     /gene="glsA"
                     /locus_tag="ACHY5W_000240"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4445211.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLVAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNES
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60459..61751
                     /gene="ybaT"
                     /locus_tag="ACHY5W_000241"
     CDS             60459..61751
                     /gene="ybaT"
                     /locus_tag="ACHY5W_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4445212.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT        15299 a        15634 c        16450 g        14528 t
ORIGIN      
        1 cgtgtaagaa aaatagaatt tataaatggc gccaataatt tccaaacgct aagccgcaca
       61 aaagaacaaa tattaataag cgatgctgat gtttatttag ccgtaataat tacggcgagt
      121 gattactaca gctaaataat atttacagat tacgtcagat gcgtttttcg tagcgccaga
      181 taatccagca aaccaccaag cacgacgcca gaaagggcga ctaaaacccc gacttccagc
      241 agagcaggca ggaaagagaa gtgcgtcaaa tccagcgcgt ccatcgtcaa cagcagtaac
      301 cacacggcca acaggctgac gcccgccgcc agtgtccaca tggcaaaggc ataacctgcc
      361 ggatatgcgg tacgggagat aaagttgtca tttactcgtg tatattcaag cgtctggcgg
      421 caaaccagca gcaaaataag ccctggtacg gcagcggcga cggagaatag atagaatgtc
      481 gaccagccgt gtgcttcaac aaaccaaccc gccacggggc cgacataaac tcgccctaca
      541 gcagaaagcg ctgagagcag ggcaaattga gtagcggaaa atgacttatt acatagtgtc
      601 attaacagcg cgacaaaggc tgatgtgccc atcccgccac agaggttttc gaaaaagacg
      661 gctgcgccca tgctgtagag atgcttatca gtaatcgaca gcagccagta accagcgtta
      721 gacgcacctt gtaaaatgcc gaaaatcagc agtgcccgga acagtgacag gcgctgcatc
      781 aaaatcccac cgtacaatgc gccaacaatg gtcgctagta agccaagcgt tttgttaacc
      841 acgcctactt cacccgcatc aaacccgacg ccgcgaatca aaaacgtggt tgtcaggctc
      901 atggcgaatg cgtcgcccag cttatacagc acgataagaa gcaaaataag ccaggcatta
      961 ttgcgaccaa agaaatctcg cagaggtgca acaaccgctt gttccagcgt ttttggcaca
     1021 ggaatggtgt cggttggttc tggtgcaagc aacgtcgcga taatacaggg gatcaacagc
     1081 gccgccatca accagtacat gccctgccag cccagccatt tatctgccag ccacagggct
     1141 aggccgccgg aaaccagcat ccctaaacgg taacccagca cgctgattgc cgcgcccgca
     1201 ccacgttctt ctgccgggag cacatcggtt ttccacgcat cgaagacaat atcctgggag
     1261 gcagagcaaa aagcgatcac caccgccagc gccgccatcc agcggagttg ggtgcctggt
     1321 tcgagaaaac ccatcgccgc aatggcgact aataacagga tttgcgtggc gagcagccaa
     1381 ccgcgccgcc gcccaaaaaa tgggggcgtg tagcggtcca tcagcggtga ccagaggaat
     1441 ttaaaaacgt aagcctggcc taccagagag aagaaaccaa tggttttgag atcgatattc
     1501 tcgaccgtca tccaggcctg taaggtgccg gatgtcaggg cgagcggtag cccggaagca
     1561 aaacccagga tcagcaatat ggctgaacgc ggctgttgaa aaatacgtaa atattgactg
     1621 gacatgggcg tctacaggcc cggcttgcac cgggccagag ggcagaaatt aacgcgcgtt
     1681 ctgcttgatg aattcgtgaa tgctggtgtc ctgagacata tcagcgatgg tatcggtcag
     1741 cacactgtta accgcatcgg cgatattttt gttggaggcc tggaacgcac cttcaacgtt
     1801 gtagctggca cgatagtttt tggtcatttt gttgccattc tgcgcggtag cgatgatggc
     1861 gatatccgct ttggtagcga tgttgtagcg cacgttgccc tgggacacgt cagcatacag
     1921 ttggctaacg atgatttgca gattaaccgg gccattcgga ccaaccatgt aaccacgcgc
     1981 ggtcatctgt ttttccagca cttcttgcag caggaaacgc agatcgcggg aggcggtcag
     2041 ggtaacgatt tgattatcgc gggtgacttt tgccagcgcc tgatcggtac gctgatcggc
     2101 accattaatg cttacggtga cgcccatcag gcttggatcc tgctgtggca gtgtaatcgt
     2161 cggggaaact tcaatagttg ttggcggttt tgcgcatcct gcaagcataa acagagcaac
     2221 taacgggaag aggatttttt tgaacatgtt cgggctctca gagactctta agcgtgtttg
     2281 gtaaaaattc ccgccatcat aacattgcca acggcgaggg gaagtaggta aggcatgtaa
     2341 attcatcatg ttgacgaaat aatcgcccct ggtaaaagaa acgctgatgc gaggcctgtg
     2401 tttcaatctt taaatcagta aacttcatac gcttgacgga aaaaccagga cgaaacctaa
     2461 atatttgttg ttaagctgca atggaaacgg taaaagcggc tagtatttaa agggatggat
     2521 gacatctcag cgttgtcgga ggagatattt catgatgata cgtgagcgga tagaagaaaa
     2581 attaagggcg gcgttccaac ccgtattcct cgaagtagtg gatgaaagct atcgtcacaa
     2641 tgtcccagcc ggctctgaaa gccattttaa agttgtgctg gtcagcgatc gttttacggg
     2701 tgaacgtttt ctgaatcgtc atcgaatgat ttacagtact ttagcggagg aactctctac
     2761 taccgttcat gcgctggctc tgcatactta cactattaag gagtgggaag ggttgcagga
     2821 caccgtcttt gcctctcctc cctgtcgtgg agcaggaagc atcgcgtaaa aacgcatttg
     2881 caactgtcgg cgcttttcca gtatgttgct aaagatttta tgaaaaacgg cctgcgggcc
     2941 gttttgtttt gtctggattt tgcgcttttt gcccagcatt cagacgaaaa ttgcccggga
     3001 attgtgaaaa aatacgcgac agcgcgcaat aaccgttctc gactcataaa agtgatgccg
     3061 ctataatgcc gcgtcctatt tgaatgcttt cgggatgatt ctggtaacag ggaatgtgat
     3121 tgattataag aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg ggtaagagtt
     3181 gaccgagcac tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa accactcaag
     3241 gccttggccg ccgtgtaacg attactatcg ctgctgacag catcgagacc gctgttaaaa
     3301 gcgagctggt caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa ggcaaagtgc
     3361 caatgaatat cgttgctcag cgttatggcg cgtctgtacg ccaggacgtt ctgggtgacc
     3421 tgatgagccg taacttcatt gacgccatca ttaaagaaaa agtcaatccg gctggcgcac
     3481 cgacttatgt tccgggcgaa tacaagctgg gtgaagactt cacctactct gtagagtttg
     3541 aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa aaaccgatcg
     3601 ttgaagtgac cgacgctgac gttgacggca tgctggatac tctgcgtaaa cagcaggcga
     3661 cctggaaaga aaaagacggc gctgttgaag cagaagaccg cgtaaccatc gacttcaccg
     3721 gttctgtaga cggcgaagag ttcgaaggtg gtaaagcgtc tgatttcgta ctggcgatgg
     3781 gccagggtcg tatgatcccg ggctttgaag acggtatcaa aggccacaaa gctggcgaag
     3841 agttcaccat cgacgtgacc ttcccggaag aataccacgc agaaaacctg aaaggtaaag
     3901 cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg gaactgactg
     3961 cagaattcat caaacgtttc ggcgttgaag atggttccgt agaaggtctg cgcgctgaag
     4021 tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg taaccgcgtt aagtctcagg
     4081 cgatcgaagg tctggtaaaa gctaacgaca tcgacgtacc ggctgcgctg atcgacagcg
     4141 aaatcgacgt tctgcgtcgt caggctgcac agcgtttcgg tggcaacgaa aaacaagctc
     4201 tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg ccgcgtggtt gttggcctgc
     4261 tgctgggcga agttatccgc accaacgagc tgaaagctga cgaagagcgc gtgaaaggcc
     4321 tgatcgaaga gatggcttct gcgtacgaag atccgaaaga agttatcgag ttctacagca
     4381 aaaacaaaga actgatggac aacatgcgca atgttgctct ggaagaacag gctgttgaag
     4441 ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag ctgatgaacc
     4501 agcaggcgta atttacgcag cataacgcgc taaattcgca caaaggcccg tcaccgccag
     4561 gtggtgggct tttttttgtc atgaattttg catggaaccg tgcgaaaagc ctctttcggt
     4621 gttagcgtaa caacaaaaga ttgttatgct tgaaatatgg tgatgccgta cccataacac
     4681 agggactagc tgataatccg tccataaggt tacaatcggt acagcaggtt ttttcaattt
     4741 tatccaggag acggaaatgt catacagcgg cgaacgagat aactttgcac cccatatggc
     4801 gctggtgccg atggtcattg aacagacctc acgaggtgag cgctcttttg atatctattc
     4861 tcgtctactt aaggaacgcg tcatttttct aactggccag gttgaagacc acatggctaa
     4921 cctgattgtg gcgcagatgc tgttcctgga agcagaaaac ccagaaaaag atatctatct
     4981 gtacattaac tccccaggcg gggtgattac tgccgggatg tctatctatg acaccatgca
     5041 gtttatcaag cctgatgtca gcaccatctg tatgggccag gcggcctcga tgggcgcttt
     5101 cttgctgacc gcaggggcaa aaggtaaacg tttttgcctg ccgaattcgc gcgtgatgat
     5161 tcaccaaccg ttgggcggct accagggcca ggcgaccgat atcgaaattc atgcccgtga
     5221 aattctgaaa gttaaagggc gtatgaatga acttatggcg cttcatacgg gtcaatcatt
     5281 agaacagatt gaacgtgata ccgagcgcga tcgcttcctt tccgcccctg aagcggtgga
     5341 atacggtctg gtcgattcga ttctgaccca tcgtaattga tgccagaggc gcaactgtgc
     5401 cgctatactt atccagggcg gcacaacgct gtaagcggct tgcgcctgag aatggcattt
     5461 gcgtcgtcgt gtgcggcaca aagaacaaag aagaggtttt gacccatgac agataaacgc
     5521 aaagatggct caggcaaatt gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg
     5581 cgcaagctga ttgccggtcc atccgtgtat atctgcgacg aatgtgttga tttatgtaac
     5641 gacatcattc gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg
     5701 acgccgcatg aaattcgtaa ccacctggac gattacgtta tcggccagga acaggcgaaa
     5761 aaagtgctgg cggtcgcggt atacaaccat tacaaacgtc tgcgcaacgg cgataccagc
     5821 aatggtgtcg agttgggcaa aagtaacatt ctactgatcg gtccgaccgg ttccggtaaa
     5881 acgctgctgg ctgaaacgct ggcgcgcctg ctggacgtcc cgttcaccat ggccgacgca
     5941 accacgctga ccgaagccgg ttatgtgggc gaagacgttg aaaacatcat tcagaagctg
     6001 ttgcaaaagt gcgattacga cgtacagaaa gcgcagcgcg ggattgtcta catcgatgaa
     6061 atcgacaaga tttctcgtaa gtcagacaac ccgtctatta cccgtgacgt ttccggtgaa
     6121 ggcgtacagc aggcactgtt gaaactgatc gaaggtacgg tagctgctgt tccaccgcaa
     6181 ggtgggcgta aacatccgca gcaggaattc ttgcaggttg atacctctaa gatcctgttt
     6241 atttgtggcg gtgcgtttgc cggtctggat aaagtgattt cccatcgtgt agaaaccggc
     6301 tccggcattg gttttggcgc gacggtaaaa gcgaagtccg acaaagcaag cgaaggcgag
     6361 ctgctggcgc aggttgaacc ggaagatctg atcaagtttg gtcttatccc tgagtttatt
     6421 ggtcgtctgc cggttgtcgc aacgttgaat gaactgagtg aagaagctct gattcagatc
     6481 ctcaaagagc cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa tctggaaggc
     6541 gtggatctgg aattccgtga cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt
     6601 aaaaccggtg cccgtggcct gcgttccatc gtagaagccg cactgctcga taccatgtac
     6661 gatctgccgt ccatggaaga tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt
     6721 caaagcaaac cgttgctgat ttatggcaag ccggaagcgc aacaggcatc tggtgaataa
     6781 ttaaccattc ccatacaatt agttaaccaa aaagggggga ttttatctcc cctttaattt
     6841 ttcctctatt ctcggcgttg aatgtggggg aaacatcccc atatactgac gtacatgtta
     6901 atagatggcg tgaagcacag tcgtgtcatc tgattacctg gcggaaatta aactaagaga
     6961 gagctctatg aatcctgagc gttctgaacg cattgaaatc cccgtattgc cgctgcgcga
     7021 tgtggtggtt tatccgcaca tggtcatccc cttatttgtc gggcgggaaa aatctatccg
     7081 ttgtctggaa gcggcgatgg accatgataa aaaaattatg ctggtcgcgc agaaagaagc
     7141 ttcaacggat gagccgggtg taaacgatct tttcaccgtc gggaccgtgg cctctatatt
     7201 gcagatgctg aaactgcctg acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc
     7261 gcgtatttct gcgctctctg acaatggcga acacttttct gcgaaggcgg agtatctgga
     7321 gtcgccgacc attgatgagc gagaacagga agtgctggtg cgtactgcaa tcagccagtt
     7381 cgaaggctac atcaagctga acaaaaaaat cccaccagaa gtgctgacgt cgctgaatag
     7441 catcgacgat ccggcgcgtc tggcggatac cattgctgca catatgccgc tgaaactggc
     7501 tgacaaacag tccgttctgg agatgtccga cgttaacgaa cgtctggaat atctgatggc
     7561 aatgatggaa tcggaaatcg atctgctgca ggttgagaaa cgcattcgca accgcgttaa
     7621 aaagcagatg gagaaatccc agcgtgagta ctatctgaac gagcaaatga aagctattca
     7681 gaaagaactc ggtgaaatgg acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat
     7741 cgacgcggcg aagatgccga aagaggcaaa agagaaagcg gaagcagagt tgcagaagct
     7801 gaaaatgatg tctccgatgt cggcagaagc gaccgtagtg cgtggttata tcgactggat
     7861 ggtacaggta ccgtggaatg cgcgcagcaa ggtcaaaaaa gacctgcgtc aggcgcagga
     7921 aatccttgat accgaccatt atggtctgga gcgcgtgaaa gatcgcatcc ttgagtacct
     7981 cgcggttcaa agccgtgtca acaaaatcaa gggaccgatc ctctgcctgg tagggccgcc
     8041 gggggtaggt aaaacctccc tgggccagtc catcgccaaa gccaccgggc gtaaatatgt
     8101 ccgtatggcg ctgggcggcg tgcgtgatga agcggaaatc cgtggtcacc gccgtactta
     8161 catcggttct atgccgggta aactgatcca gaaaatggcg aaagtgggcg tgaaaaaccc
     8221 gctgttcctg ctcgatgaga tcgacaaaat gtcttctgac atgcgaggcg atccggcctc
     8281 tgcactgctt gaagtgctcg atccagagca gaacgtagcg ttcagcgacc actacctgga
     8341 agtggattac gatctcagcg acgtgatgtt tgtcgcgacg tcgaactcca tgaacattcc
     8401 ggcaccgctg ctggatcgta tggaagtgat tcgcctctcc ggttataccg aagatgaaaa
     8461 actgaacatc gccaaacgtc acctgctgcc gaagcagatt gaacgtaatg cactgaaaaa
     8521 aggtgagctg accgtcgacg atagcgccat tatcggcatt attcgttact acacccgtga
     8581 ggcgggcgtg cgtggtctgg agcgtgaaat ctccaagctg tgccgtaaag cggttaagca
     8641 gttactgctc gataagtcat taaaacatat cgaaattaac ggcgacaacc tgcatgacta
     8701 ccttggtgtt cagcgtttcg actatggacg cgcggataac gaaaaccgtg tcggtcaggt
     8761 aaccggtctg gcgtggacgg aagtgggcgg tgacttgctg accattgaaa ccgcgtgcgt
     8821 tccggggaaa ggcaaactga cctataccgg ttcgctcggt gaagtgatgc aggagtccat
     8881 tcaggcggcg ttaacggtgg ttcgtgcgcg tgcggaaaaa ctggggatca accctgattt
     8941 ttacgaaaaa cgcgacatcc acgtccacgt accggaaggt gcgacgccga aagatggtcc
     9001 gagtgctggt attgctatgt gcaccgcgct ggtttcttgc ctgaccggta acccggttcg
     9061 tgccgatgtg gcaatgaccg gtgagatcac tctgcgtggt caggtactgc cgatcggtgg
     9121 tttgaaagaa aaactactgg cagcgcatcg cggcgggatt aaaacagtgt taattccgtt
     9181 cgaaaataaa cgcgatctgg aagagattcc tgacaacgta attgccgatc tggacattca
     9241 tcctgtgaag cgcattgagg aagttctgac tctggcgctg caaaatgaac cgtctggcat
     9301 gcaggttgtg actgcaaaat agtgacctcg cgcaaaatgc actaataaaa acagggctgg
     9361 caggctaatt cgggcttgcc agcctttttt tgtctcgcta agttagatgg cggatcgggc
     9421 ttgcccttat taaggggtgt tgtaagggga tggctggcct gatataactg ctgcgcgttc
     9481 gtaccttgaa ggattcaagt gcgatataaa ttataaagag gaagagaaga gtgaataaat
     9541 ctcaattgat cgacaagatt gctgcagggg ctgatatctc taaagctgcg gctggccgtg
     9601 cgttagatgc tattattgct tccgtaactg aatctctgaa agaaggggat gatgtagcac
     9661 tggtaggttt tggtactttt gccgttaaag agcgtgctgc ccgtactggc cgcaacccgc
     9721 agaccggtaa agagatcacc atcgctgccg ctaaagtacc gagcttccgt gcaggtaaag
     9781 cactgaaaga cgcggtaaac taagcgttgt ccccagtggg gatgtgacga agttcaaggg
     9841 cgcatctact gatgtgcctt ttttatttgt attcggtgac tttctgtgtc ttgtgggctg
     9901 acaattgccc ccgtttcttg tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg
     9961 tacacagtca tctacagcgg agtgttgtta caccatgatg gacagcttac gcacggctgc
    10021 aaacagtctc gtgctcaaga ttattttcgg tatcattatc gtgtcgttca tattgaccgg
    10081 cgtgagtggt tacctgattg gcggaggcaa taactacgcc gcaaaagtga atgaccagga
    10141 aatcagccgt gggcagttcg agaatgcctt caacagcgag cgtaatcgca tgcagcaaca
    10201 gctgggcgat caatactccg agctggcagc gaacgaaggc tatatgaaaa ccctgcgtca
    10261 acaggtgctg aatcgtctga tcgacgaggc gctgctggat cagtacgcac gtgagctgaa
    10321 actgggtatc agcgatgagc aggttaaaca ggcgattttc gcgaccccag ccttccaggt
    10381 tgacggcaaa tttgataaca gccgctataa cggtatcctc aaccagatgg ggatgaccgc
    10441 cgatcagtac gcccaggcgc tgcgtaacca gctcactacc caacagctga ttaacggcgt
    10501 tgccggtacc gattttatgc tgaaaggtga aaccgacgag ctggcggcac tggtcgctca
    10561 acaacgcgtg gtacgtgaag cgactatcga tgttaacgcg ctggcggcga agcagcctgt
    10621 gaccgaacag gagattgcca gctactacga acaaaacaaa aacaatttca tgacgccgga
    10681 acaattccgc gtgagttaca tcaagctgga tgccgcaacg atgcagcaac cggttagcga
    10741 tgcggatatc cagagctact acgatcagca tcaggatcaa ttcacccagc cgcagcgtac
    10801 ccgctacagc atcatccaga ccaaaactga agatgaagcg aaagcggtac ttgatgagct
    10861 gaataaaggc ggtgattttg ctgcattagc caaagaaaaa tctgccgata ttatctctgc
    10921 tcgtaacggc ggcgatatgg gttggttaga agatgccact atcccggatg aactgaaaaa
    10981 tgctggtctg aaagaaaaag gccaactgtc tggtgtcatc aaatcttcgg tcggtttcct
    11041 gattgtacgt ctggacgaca ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga
    11101 cgacattgcg gcgaaagtga aacacgaaaa agctctcgat gcgtactacg cgctgcagca
    11161 gaaagtgagc gatgcggcaa gcaacgacac cgagtctctg gccggtgcag agcaagctgc
    11221 cggcgttaaa gccactcaga cgggttggtt cagcaaagat aacctgccgg aagagttgaa
    11281 cttcaagccg gttgccgacg ctatctttaa cggcggtctg gtaggtgaaa acggcgcgcc
    11341 gggcatcaac tctgacatca tcaccgtaga cggcgaccgc gcattcgtgc tgcgcatcag
    11401 cgagcacaaa ccggaagcgg tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact
    11461 ggttcagcac aacaaagctg aacaacaggc gaaagtggat gctgagaaac tgctggttga
    11521 tttgaaagcc ggcaaaggtg cggaagctat gcaggctgcc ggtctgaaat ttggcgagcc
    11581 gaaaacctta agccgttccg gtcgtgaccc gattagccag gcggcgtttg cactgccact
    11641 gccagcgaaa gacaaaccga gctacggtat ggcgaccgat atgcaaggta atgtggttct
    11701 gctggcgctg gatgaagtga aacaaggttc aatgccggaa gatcagaaaa aagcgatggt
    11761 gcagggtatc acccagaaca acgcacaaat cgtctttgaa gctctgatga gtaacctgcg
    11821 taaagaggcg aaaatcaaaa ttggcgatgc gttggaacag caataatcct gaagccgcct
    11881 cgcaaaaaaa tgctttgcag ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt
    11941 ctgaacattg ccatttgttt actgttttca ctcccgttaa ggtgattcca ctgttaacaa
    12001 acaaggagaa aacagtatga aacacggaat taaagcactg ctcattaccc tgtccctggc
    12061 ctgtgccgga atgtctcata gcgcgctggc ggcagcttct gtggcgaaac cgacggcggt
    12121 agaaaccaaa gcggaagctc ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag
    12181 tgacgaagaa ggcacccggg tcagcattaa taatgccagc gcggaagagc tagcccgcgc
    12241 gatgaatggc gttggcctga agaaagcgca ggcgattgtc agttatcgcg aagagtacgg
    12301 tccgtttaaa acggtggagg atctaaagca ggtgccgggg atgggcaatt cgctggtgga
    12361 acgtaatctg gcggtattaa ccctgtaatt aatttgcata gtggcaattt tttgccagac
    12421 tgaagaggtc ataccagtta tgacctctgt acttataaca gtttttataa caacaacgta
    12481 aggttattgc gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca
    12541 gcacgtaaac aacgctcgct accttgaatt cctcgaagaa gcccgctggg atgggttgga
    12601 aaatagcgac agttttcagt ggatgacggc ccataacatc gctttcgtgg tggtgaatat
    12661 caatattaac tatcgtcgtc cggcggtatt aagcgacttg ttaaccatta cgagccagtt
    12721 acagcaatta aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg
    12781 gcaggtggta gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc
    12841 attagctctg gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg
    12901 tggtgccgga tgctcaagcc gcatccggcg acacccggaa taattacttc aacccggttt
    12961 tctgcttcat cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg
    13021 cgcgtaaatt acacgccgca caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg
    13081 tcaacgtttc gttacggact aaatccagtt tgccgtaata atctgccagc gcccaggttt
    13141 ccgctttatc aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac
    13201 tgacggcatg gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat
    13261 ccgtttcgca gacgccagta attacggctt ctgcttttac ctgatacgca tatattgccg
    13321 ccagcgtcag gaacaaaata ttacgccctg ggacaaacgt attcgggatg ccatcagctt
    13381 caggttcata atcaggcacc ggaatgctgt cacgcgtcag gctgctgacc gccagctcgt
    13441 tgagcagcgt tacatcaaga accttatgcg cgcgtgcccc cagtttcagc gccagttcgc
    13501 gtgccacgtc gatttctgcg cgatggcgct gaccgtaatc gaacgtcacg caatggactt
    13561 catcatattg ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca
    13621 caacgacagc acgtttcata gataatcctg cctgaacaat aaaagcgtta tggtaacgcc
    13681 tgcgattaac ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat
    13741 tcaaaccagc cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc actaatgcga
    13801 tagtgatagt tgaacagcgg cgtcagcgtc gcgtctgcca ttaactggct aaaaaccgct
    13861 ttcagggcat taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagtgtcgat
    13921 tgcaaatgtg catatgctgg agcgtcgaaa acatgtggcc atagtggatc gcagcgcagc
    13981 cattgctcca gcgtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct
    14041 gcgtgtgcca ataggggcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt
    14101 tcacagcctt ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc
    14161 ggtaggtgat aaaccagcgt caaggttttc ggtagtttga cttcatccgg tacttgccaa
    14221 tgcggaatag tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact
    14281 tctaacgttt gtaataaacc ggattgatga ataatggaga ttacttttcg cgcctgccag
    14341 agggagagtc ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta
    14401 ctgacctggc tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc
    14461 cgacaactgg ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct
    14521 ttaagcagcg gatgacgtaa atggtaataa tcatggcttt ccaggcgcac cagctccgct
    14581 gtgaattgtg ttaagcgaaa aggacccgtg ccgatcagtg ggaattgcgg atgcgccaga
    14641 tggctgcaat agctcgccag ccggtgcgca agccagtaat cagggcgatg taaaaagaag
    14701 gtcagacact gcggatgggt gacctcaata cgcttcacgc taataaataa ttgatccagt
    14761 gctggcagtt gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg
    14821 ccgttatgcc agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagcagag
    14881 gtttcccagt gatgcgctaa atcgccaatc gggcgctggg tattattatc gaagcgggtc
    14941 aggccggaaa atatctgtcc ggcgagatgc tgctcggcac ggccgggcaa aaagcccggt
    15001 tgtagcggtt cgagcgggcg atagtaggga atacgcaacg tgggtgtatc gttttgccat
    15061 tgtccgccca taaacggctg taacagagtg cgcagctcac ttggggccag ttgcgccagc
    15121 tccagcacat cttgctgctt tccggtttcc agtgcctttt ccatcatcgc attgcgtagc
    15181 gattctggcg tgaccagaaa gcgtagttgt ccgcgctttc cgcgtcctga ctgcgcctgc
    15241 cactccagcc atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg
    15301 caaaaacagc gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt
    15361 tgccacagac gttgatactg gttaagacgg ttgagcagtc gcatataaac ccggaacaat
    15421 attatttaac tattcactat tacttccgta tatatcaggt gatactcaat caccattaac
    15481 cgtgtcacag agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt
    15541 tattgatgcc tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg
    15601 aacgcgacat taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc
    15661 tcggggcgct atcgctggat gcatatttga ttaccggcaa cggaacgcgc gtgcattctc
    15721 tggaaggtga acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt
    15781 atcagcaatg ggatacccga gcaagcatgc atatcttcaa tgacgatggt tggtttaccg
    15841 ggaaagagat ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa
    15901 tcgatgtcaa aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg
    15961 acgatcttac acgcttgcag atccagctat acgaagcatt agccgagcgt gcacatttgt
    16021 gtttttccgc cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcac
    16081 tgacggtgct gacccaacat ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg
    16141 cgatgaacga tcgcgaaatg ttaggcagcg tcggtagcgg atttattatg ggcaatgcga
    16201 tgccgcaact gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg
    16261 ctgtctctca ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat
    16321 aacgagatcc cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt
    16381 ttgtgccgtg gtgtttaaac cgcacagaat aaattgtcgt gatttcacct ttaaaataaa
    16441 attaaaagag aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct
    16501 gctggcctta ctgcagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa
    16561 tctgacaacc accccttgct ggaaacgcct gaaacggctg gaggacgacg gtatccttat
    16621 cggcaaagtc gccctgctgg atccggaaaa aataggcctc ggcctgaccg cctttgtgct
    16681 gataaaaacg caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga
    16741 aatgccagaa gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt
    16801 ccaggttgcc gacatgaaac gctacgacga gttttataag cgtctggtaa acagcgtgcc
    16861 ggggctgtcg gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt
    16921 acccatcgaa taaatatcca gaatcaagtc aggacacaac gcgtgcgatt atttgctcaa
    16981 ttaagctggt atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc
    17041 attatcgcga tgctgcaact ggttccgcca aaagtggttg gtattgttgt cgatggcgtg
    17101 acagaacaac actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt
    17161 gccgttgtgg tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg tgcgtcttat
    17221 caactggctg ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag
    17281 ttttacctgc gtcatcgcac gggtgacctc atggctcgtg cgactaatga cgtcgatcgc
    17341 gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg attcactggt aatgggctgc
    17401 gctgtgttga ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgtta
    17461 ccgatgccag tgatggcgat catgattaag cgcaacggcg atgctttgca tgaacgcttt
    17521 aagctggcac aggcggcgtt ttccagtctt aatgaccgca cccaggaaag cctcaccagt
    17581 atccgcatga tcaaagcctt tggtctggaa gatcgccagt cggcgttatt tgccgcggat
    17641 gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg
    17701 accatctata tcgcgattgg tatggcgaac ttgctggcta ttggcggcgg tagttggatg
    17761 gtggtgcagg gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg
    17821 atgatttggc caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggtagtgct
    17881 gcgtacagcc gtattcgcgc gatgctggcg gaagcgccgg tggtgaacga tggtagcgaa
    17941 cccgtgccgg aagggtgtgg cgaactggat gtaaatattc accagttcac gtatccgcag
    18001 actgaccatc ctgcactgga aaacgtcaat ttcgccctga aacccggtca gatgctgggt
    18061 atttgcgggc cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc
    18121 gacgtcagcg agggggatat tcgctttcat gatattcctc tgacgaagtt acaactcgat
    18181 agctggcgta gccgcctggc ggtagttagc cagacgccat tccttttttc tgacaccgtg
    18241 gcgaataaca tcgcgctggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg
    18301 cggttagcca gcgtacatga cgatattttg cgtctaccgc aaggttacga tacagaggtg
    18361 ggcgagcgcg gtgtgatgct ttccggcggg caaaaacagc gtatctccat tgctcgtgcg
    18421 ttattagtca acgcggaaat cctcatcctt gatgatgcgc tttcggcggt ggacggacgc
    18481 actgagcacc agatcctgca taacctgcgc cagtggggac agggaagaac ggtaatcatc
    18541 agtgcccatc gcctttcggc actgacggaa gccagtgaaa ttattgtgat gcagcacgga
    18601 catatcgccc agcgtggcaa tcatgatgag ctggcacaac aaagcggctg gtatcgcgac
    18661 atgtatcgct atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag
    18721 gccgtcgatg cgtagtttta gccaactgtg gccgactctc aagcgcctgt tagcgtacgg
    18781 ttcaccgtgg cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg cggcggcggc
    18841 agaagtcagt gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa
    18901 cctgccgttg aaagtggttg cagggctggc tgcggcgtat gttgggctac aactgtttgc
    18961 cgccgggcta cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca
    19021 acagttgcgt accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac
    19081 ccaacccgtc gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct
    19141 ctacgttacc gtagtggcaa ctgtcctgcg cagtgccgca ctggtgggcg cgatgctggt
    19201 ggcgatgttc agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt
    19261 gctggtggta atggtgatat accagcgtta cagcacgccg attgtccgtc gtgtgcgcgc
    19321 ctatctggcg gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca
    19381 gcagtttcgt cagcaggcgc gatttggcga acgtatgggg gaggccagtc gttcacacta
    19441 tatggcgagg atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct
    19501 gttttcatcg ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ccagcggcac
    19561 cattgaagtg ggcgtgttgt atgcgtttat cagctatctt gggcgactta acgaaccctt
    19621 aatcgaactg accacgcagc aggcgatgct gcaacaggct gttgttgctg gtgagcgtgt
    19681 gtttgaactg atggacggac cgcgccagca atatggcaat gatgatcgcc cgttacagag
    19741 cggcaccatc gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa
    19801 gaacattaat ctctctgtgc cttcgcgtaa ctttgtggcg ctggtcgggc ataccggcag
    19861 tggcaaaagc accctcgcca gtttattgat gggctattac ccactaacgg aaggtgagat
    19921 tcgccttgat ggtcgtccat taagttcgtt aagtcacagc gcgctgcgcc agggcgtggc
    19981 aatggtgcag caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg
    20041 gcgtgatatc tccgaagaac gcgtctggca ggcgctggaa accgtgcaac tagcggagct
    20101 ggcgcgtagc atgagcgacg gtatttacac gccgctgggc gagcagggga ataatctctc
    20161 cgttgggcaa aagcaactgc tggcactggc gcgcgtgctg gtggagacgc cgcaaatcct
    20221 gatccttgat gaggcaaccg ccagcattga ctccggtact gaacaggcga ttcaacatgc
    20281 tctggcggcg gtgcgtgaac atacgacgct ggtagtgatt gctcaccgct tatcgaccat
    20341 tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca
    20401 ccagcaactg ctggcggccc agggacgcta ctggcagatg tatcaactgc aacttgcggg
    20461 cgaagagctg gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa tagcgcaata
    20521 tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcacagt catagtgcgt tttcattttc
    20581 aatcttctta acttcctgct ctctttctcg tttttcattt ctggcacacc gcttgcaata
    20641 ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt
    20701 gaccgtgata atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg
    20761 tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga
    20821 gctgtaccgg ggggcggaat acagtgtcaa cttcctgcca aaagtaaaaa ttgatgtggc
    20881 gattgctgat gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg
    20941 aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac
    21001 cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg
    21061 aagatagcga cgataaaaac tgggcttgct tcactggcga tgctgccggg actggtaatg
    21121 gctgcacctg cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg
    21181 gtgctgttta tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa
    21241 aacgtgctgt cgatgctgac gcaggtgacg gtgacatttg cactggtctg tattctctgg
    21301 gtggtttacg gttactcgct ggcgtttggt gagggcaaca acttcttcgg caacattaac
    21361 tggttgatgc tgaaaaacat cgaactgacg gcggtgatgg gcagcattta tcagtatatc
    21421 cacgtagcgt ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggggcgctg
    21481 gcggaacgaa tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctct
    21541 tacattccga ttgcgcatat ggtgtggggc ggtggtttgc tggcttctca cggtgcgctg
    21601 gatttcgcgg gtggcacggt ggtgcatatt aacgccgcaa tcgccggtct ggtgggcgcg
    21661 tatctgatag gcaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca caacctgccg
    21721 atggtcttca ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca
    21781 gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga atactgtgct cgcaacggcg
    21841 gcggcaattc ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg
    21901 ggggcgtgtt ctggcgcgat tgccggtctg gtcggcgtga cgccagcctg tggctacatt
    21961 ggtgttggcg gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt
    22021 accatgctca aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc
    22081 gtttgtggca ttgtcggctg tatcatgacc gggatttttg ccgccagctc gctgggcggc
    22141 gtgggcttcg ctgaaggtgt gacgatgggc catcagttgc tggtacagtt ggaaagcatt
    22201 gccattacga tcgtctgctc cggtgtggtg gcatttattg gctacaaact ggcggatctg
    22261 acggttggcc tgcgtgtgcc ggaagaacaa gagcgagaag ggctggacgt caacagtcac
    22321 ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct
    22381 ccggctattt tttaattgtg attacgcatc accccttcct gaacggtcga ggcaaccagt
    22441 acgccgtcct gggtataaaa ctcaccgcgc acaaagccac gtgcgctgga cgccgaggtg
    22501 ctctccacgc tatacagcag ccattcattc aaattaaacg ggcgatggaa ccacatggaa
    22561 tggtcaatgg tggcaatctg aatccccggt tcgagaaaac cgatgccgtg cggctgtaga
    22621 gctaccggca ggaagtttag atcagaagcg taaccgagca gatactgatg aacgcgcagg
    22681 tcatccggca cgctaccatt tgcgcggatc cacacctgac gatgtggttc tgcgacgtga
    22741 cctttcagtg ggttatgaaa ctccaccgga cggacttcca gcggacgatc gcagatgaat
    22801 ttatctttaa gcactggcgg cagcaggtgc gccagcgatt gggtgatttg cgtttccgaa
    22861 ggcaggccat caggcgctgg cgcggacggc attgtttttt gatgttcgaa acccgcttct
    22921 ggtgcctgga aagaggccgt catataaaaa atcggtttgc cgttttgaat agcagcaacc
    22981 cggcgggcgc tgaagctgtt accgtcacgc agcgtttcga catcataaat aatcggcttc
    23041 ttactatcgc cagggcgaag aaagtagctg tgaaacgaat gtaccagccg ctcttcaggg
    23101 acggtctctt ttgcagcata caaggcctga cccacgacct ggccgccaaa tacctggcgt
    23161 aaacctaaat cttcactctg gccgcgaaag agtccttcct caattttttc cagatttaac
    23221 aatgtcagta aattttttag cgcctgactc atataactct ccagtaacaa agctgccgca
    23281 gcaagccaaa gtgagttgag tataacgcaa atttgctact ggtccgatgg gtgcaatggt
    23341 ctgaattacg ggctaattac aggcagaaat gcgtgatgtg tgccacactt gttgacgtta
    23401 ctattttgtt aaccactctt ccggcgagga aagttagccc gctggtgcat tgataataag
    23461 gagaaatgaa tgaaactcgt gcacatggcc agtggtgtag cggttgcgat tgcgttggcg
    23521 gcttgcgcag ataaaagcgc ggatattcag acgccagcac cggctgcaaa tacgtctatt
    23581 tcagcaacac aacaacctgc tatccagcaa ccgaatgtct ccggtaccgt ctggatccgt
    23641 cagaaagtcg cactgccgcc tgatgccgtg ctgaccgtga cactttctga cgcgtcgtta
    23701 gccgatgcac cgtcaaaagt tctggcgcag aaagcggtgc gtactgaagg taaacagtca
    23761 ccattcagct ttgttctgcc atttaacccg gcagatgttc agccgaacgc gcgtattctg
    23821 ttgagtgcgg cgattaccgt gaatgacaaa ctggtattta tcaccgatac cgttcagccg
    23881 gtgatcaacc agggcggaac taaagccgac ctgacattgg tgccggtaca gcaaaccgcc
    23941 gtgccggttc aggccagcgg tggcgcaacg actaccgtac cttcgacttc accaactcag
    24001 gtgaatccgt cttcggcagt tccagctcct acgcaatatt aagcgacgtt aaccctctcc
    24061 gcccggagag ggttagtagt tccagcgata acgctgcaag tcgatttgcc cgcttcccga
    24121 taccatcaca ccttctgcca gtaatgcctg tcgctgacgc tgtaaatccg gtccggttag
    24181 cgaaattgtg ccgtggcgat taaccacccg gtgccagggt aaggtgctgc cttcagggag
    24241 acgctttaac acaccgccca cctggcgcgc ggcgcggggc gatcccgcca gtttcgccac
    24301 atcaccgtaa gtggttacat agccttcggg aatagcggcg acgatttgcc agacgcgttg
    24361 gggaaatgaa tcttcttttt ccatcttttc ttcctgaggt aatttttcag cataatctgg
    24421 aaaaacgccc gagtgaagtc gcattgcgca agaaaccagc atctggcacg caatgggttg
    24481 caattagccg gggcagcagt gataatgcgc ctgcgcgttg gttctcaacg ctctcaatgg
    24541 gggctctgtt ggttctcccg caacgctact ctgtttacca ggtcaggtcc ggaaggaagc
    24601 agccaaggca gatgacgcgt gtgccgggat gtagctggca gggcccccac ccatttctgc
    24661 ctcccaccgt ttcgtcaaaa aatcccaaca tggctaaact ttaaccacaa ctgacgtcgc
    24721 aagaattgtc tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt
    24781 tttgtggttg ccgttcctgc cgataaaaag gatgcctatc gggaaatggc cgctaaggcc
    24841 gcgccattgt ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc cagcgatgta
    24901 ccggatggca aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt
    24961 gtctttagct ggattgaata cccttcaaaa gaggtccgcg acgcagctaa tcaaaagatg
    25021 atgtcggacc cacggatgaa agagttcggc gagtccatgc cgtttgacgg caagcgaatg
    25081 atctatggcg gattcgagtc aatcatcgac gaataggtag cgtgacgggc tgtgcggcgt
    25141 gccgtcagcc caaaaattca caaatgttgc tcggcccagc gtaagaaatc ttctttcggt
    25201 aatgccttgc tgtagagcca gccctgaccg tagtgcacgc catgctggcg tagccactct
    25261 tcttgtttac tggtttcgat tccctccgct accattttca gtttcagtgt ttttgccatt
    25321 tcgatgatat gcggcgtgac atttttatat tccagcgcat caacgaaaga tttatcgatc
    25381 ttcagaatgt cgacatccag atcctgtaaa taacttaaac ttgaataccc cgtaccaaaa
    25441 tcatcaagat aaatttcatg gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt
    25501 ttcggatcgg caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg attaacctga
    25561 tagtgattga tcatttcacg cagcaattgc gggatttttt ccgaggtgag cacggtggat
    25621 tcaagattga tcgaaatatg ctgctgtgga tgctgacgca gccagtcgcc catatcttca
    25681 aagacgcttc ttataatcag tagcgtcaat ggctcagaaa ggcccgtttg ctgtgccagc
    25741 ggaataaaac tatctggtga caaccaacta ccgtctgtct gcggccagcg cgccagtgcc
    25801 tcagcaccga caattttgcc attggctaag gagacaatcg gctgatagtg cacgcaaata
    25861 tcacgatttt cgatagcatc ctgcagccga tgatgcggtg actggatacg gcgcaaaata
    25921 cgcagcacaa acatcgctgc cagcaggcca atcaccaacc cgagcggtaa ccaaataaag
    25981 acttgccgat gccagccttt ctgcaacatt ttcgttgaag cccaggtgat gatcgaaata
    26041 ttcatctccg ggaagggcag gatatcgtaa atgattccat tattttcgat atgctcaccg
    26101 ggtgtttttt gtagcctggt aataattccc tgagcaattt catcgctgct ggttatgaca
    26161 acgttatggg cattgccaat catggcggca tcaatttgcc atgagcgata gggaatgaca
    26221 tcaataaagg aagcggggtc gatcatgacg acataatgtg ccgttcccat ggcgaccatg
    26281 taacggataa tgcctaaatc gttatgcgat gttaaccaga cacgataacc atctttcgaa
    26341 attttacctg gctcggggaa ggtatcgggc ggactttcat gctccagaga cgaacactgg
    26401 ggaacgttgt tatcgatata aaccacttcc tgaatatagc gataactgta agatacccga
    26461 cgcatttcca tgagatgggc ttcgctacag gcagcgcctt gccatctttc cagctcctgc
    26521 agcgcatctt tcccttgtgt cgccacctta ttggcccgaa tagcgacgcg ggaggaatag
    26581 gtattcagct cttcaataaa cgatgtttct acctgctgat gggccagcca gatgcttaag
    26641 ccgacaggca gcaatactga aagaatcagt actcccgaaa taaggccgac cagatgtcgt
    26701 gttctcacca gcatatcctt attaacacag tgtagggaaa gtatatctga ttgtggaaaa
    26761 aggctggcag gagataagga ggtagagctg gtatttcatg aagtgatggg tggaaacgat
    26821 cgttgtcatt tgaccaggcg cagagcttta cgcctggtat gtaaccctaa cttaagatgt
    26881 tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac agttcacctt tacggacgat
    26941 cattttttgc agttgttcct tatgaacatc cgcgacacgc ggagagttca agatggcttc
    27001 aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg ccgttatagc aaacgcggaa
    27061 atccagcacg tcgatatctt catagcggta gcgtgggtag acatcgaaaa agaagaaacc
    27121 attaagcagg ataaataata caaccagtag ccagacagag cctgaaagcg tttcggactg
    27181 caacatcacc gccagggtgg caaaaaaagc aacgtacata ccgataaaca gccccggatg
    27241 tttacggata aaactgatac taaagcgggg tttgttgtcg cgtttttcgc gggtattcag
    27301 agactcaatc gtttcggtca gcaggcgttg tatttcggtc atcgtttgcc ttcgtggtat
    27361 ctgcaacttt ccagggaaca ccctatttta acgtgggaat agtagaggga aagtaacaga
    27421 tttaccacaa ttttgcataa cagttgcgaa aaaccgatta caatttttta attattctgg
    27481 ctggattacc gcccacgaca acgttgtccg ggacatcttt tgtgacaact gcacctgagg
    27541 ctaccacgac gttatcacca atggtcacac cagggttaat gaccgcgcgt ccgccaatcc
    27601 agacgttatt accgatggtg acgggtttcc ccagttcagc accgctatta cgtgctacag
    27661 ggtcgatggg atgtgttgcc gtgtagatat gaacgcctgg tgccaacata cagttatcac
    27721 cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa gttggcgaaa aaattattac
    27781 cgagaaaaat gttatagcca tagtcacagc gaaacgttgg ctcaatataa gcctctgtca
    27841 cctgaccgaa tagatcagcg agaatttgct ggcgtaatgt gtgctcttcc gccagggaat
    27901 gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc gcgagataac gtctcatctg
    27961 ccgagcgata caactcacca gcaatcatct tttctttttc tgtgctcatt actgacctcc
    28021 ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca gtgaagagaa cgttcccatt
    28081 acatctgtgt gataaagatc acatagggta tgtcctcatg acgaaaaagt ataaaattct
    28141 taataaacag ccggttatag ctccgaaagc gaattattga ttagcgaata aatttccata
    28201 ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa ttcggcgagg cggtgatctg
    28261 cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa
    28321 cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa tcggttttcg
    28381 ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc
    28441 gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc tgacttccgc
    28501 catttctgaa gatcctgcat attaatacca taactactga acaacataaa ggtgtcatcc
    28561 agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta
    28621 agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt
    28681 gggtcgttaa cccagccatg attgctttct tcaaggtttg caaggcagtc atgatacaga
    28741 gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata
    28801 acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa aaaacttact
    28861 gacctggact tgccctctcg tttgttagct taactataag ccactctttg caggttttca
    28921 tcgcattatt acgaaaaatt acaattagga aggaagcggg acagggctca ctttttcata
    28981 ctagcaccaa cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagga
    29041 ttatcgccag atgaaattgg tgacactaat accaggattg ctctgaatat gacgtaataa
    29101 ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa tgattatgga
    29161 ttattaaatc tattagcgct acttaatata aattaactaa caatcagtat tttttatgaa
    29221 ttttatacgt ggttaatact ggttttcgta tgagatcctg agttggtggt tcaattactc
    29281 cttaatgttc gtaggttatg cataaaaaag gccgcttacg cggccttagt gattacacgt
    29341 tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc
    29401 ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca
    29461 ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac
    29521 tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg atcggacgta
    29581 aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca
    29641 agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca atggttgtga
    29701 gcaggcctac ctggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg
    29761 caccgataac ccccagcgga acgaccagca taactgagaa cggaatcgac cagctctcgt
    29821 acagcgccgc cagacacagg aacacgacaa tcaacgaaat cgcgtacagt gaaggtgcct
    29881 ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca acaccggtag
    29941 gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt ttacccggtg
    30001 ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga cgcggcgaac
    30061 cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca tcagcagcac
    30121 gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt
    30181 aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctgccg ccccatgcag
    30241 cgcccagagt ggtgttaatg tcgttgatag aaacacccag cgcctgcgct ttttcctggt
    30301 cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcaaca
    30361 tatcagggtg cttcgctgct tctgcaagca actggttacg cgcctgagtc agtttttcgt
    30421 gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcggta cccagttcca
    30481 cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcgcgtg
    30541 ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct
    30601 tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg aagccgttaa
    30661 cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat
    30721 tgagcacttt ctgtgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa
    30781 acacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacgaac agataggcca
    30841 tgccgaccac gatgatcaga tacagcacca ggtaacgccc cgtactgcgc agaataccgc
    30901 ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac cagccgaaga
    30961 agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa
    31021 gagctggagt caggatcaac gccaccagta ccgacagcgc cattgctgaa acaatggtaa
    31081 tagagaactg acgatagata gcaccagtag aaccgccaaa gaaggccatc ggtacgaata
    31141 ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac
    31201 gggtggcttc ttttggcggc aaaccttctt ccgccatcac acgctcaacg ttttctacca
    31261 caacgatagc gtcatccacc aacaggccga tggcgagcac catcccgaac attgttagcg
    31321 tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat accaccggta
    31381 cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga
    31441 acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag attttcacga
    31501 acggcgtggt gtcgtacggg taaacaattt tcagacccga cgggaagaac ggttccatct
    31561 tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct
    31621 tgatccccag accggaagcc ggttggccgt taaactctgc gatgatgtcg tagttctcac
    31681 cacccagctc aatcttcgcc acatcacgca gcagcacgcg ggaaccatcc tgattcactt
    31741 tcagcaggat tttgccaaac tcttcagtag aggtcagacg cgtctgagca ataatagagg
    31801 cgttaagctg ttggcctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg
    31861 cgttctgcgc tttgatggcg gtaataacat caaccggtgt tagctggaat ttgttcagtt
    31921 cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga acgtcaccca
    31981 cgcccgacgt acggctgatg gcatctttca tattcgccgc cacgtagtcg gagatatcct
    32041 cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg
    32101 atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc atcgccagct
    32161 gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca
    32221 gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga
    32281 taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag
    32341 cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca
    32401 ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg
    32461 caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa
    32521 gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact
    32581 tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat tactacgcga
    32641 tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg gcttgcaacg
    32701 atcggacggg tttccacttt gtcatccgcg ccaactacca gtacggtggc atcgccacgc
    32761 ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc
    32821 agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg
    32881 cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc cagcgtaccg
    32941 tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttctct
    33001 tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt gctggactgg
    33061 gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg ccagcgcagt cgcctgaccg
    33121 ttctgtacca atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggagag
    33181 gtgactttgg tgtaagccag attgatccgc gcagtttcaa cggcagcttt cgccgcagtt
    33241 accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtactcttg cttactgatg
    33301 tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca
    33361 gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata ggtcgcagga
    33421 tcaatctgat agagagagac acctgcttcg atgtcgctac cttctttgaa attacgcttc
    33481 aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgaccc
    33541 ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc
    33601 atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct
    33661 gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct
    33721 cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta
    33781 tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa
    33841 ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc
    33901 tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggcgaga
    33961 ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg
    34021 atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta gagcttgagt
    34081 atcaggcaaa attccctggc gatccactct cagtattaag agagatatta attcatgttc
    34141 ttgaatccac ggtgacagaa gaacggcgtc gattattgat ggagattata ttccacaaat
    34201 gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa
    34261 gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg
    34321 atttaatgac gcgtcgcgca gcaattatta tgcgcggcta tatttccggc ctgatggaaa
    34381 actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgttgcca
    34441 tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact aacgaataac
    34501 cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc tggttcactg
    34561 cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca
    34621 ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt
    34681 attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat
    34741 ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa taaacaaaaa
    34801 gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt agccaccctc
    34861 gataaaatcg atcgcgtaaa agaagagacc gttcagctac ggcaaaaagt cgctgaagcg
    34921 ccggtaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt cgataacgac
    34981 gaagaaacgc gcaaaattct gagcacgctt tcgttgcgcc agctggaaac tcgcgttgcc
    35041 caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg
    35101 gtttcgttac agacacagcc cgaacgcgtg caaaatgcga tgtataacga ttcgcagcag
    35161 ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagactgc cttacgtccc
    35221 agccagaaag tgttaatgca ggctcagcag gcgttgctga atgcggagat tgaccagcag
    35281 cgtaaaagcc tggaagggaa taccgtcttg caggatacct tgcaaaagca acgtgattac
    35341 gtgacggcga acagcgcccg tctggagcac cagttgcagc tgttgcaaga agcggtaaac
    35401 agcaagcgcc tgactttaac cgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc
    35461 gcgcgtattc aggctaatcc gctggtgaag caggaactgg aaattaacca gcagttaagt
    35521 cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc
    35581 aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc
    35641 ctgaagggta gcctgctgtt gtctcgtatc ctttaccaac aacaacaaac gctgccctcg
    35701 gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga acagtttgaa
    35761 gttaaccagc agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa
    35821 ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg
    35881 cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat gatggccatt
    35941 aacctgcaaa tcaaccagca acagttaatg agtgtgtcga aaaacctgaa atccatcctg
    36001 actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg gatcaaagcg
    36061 ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa ttacggtgaa ctgggaaaaa
    36121 gcctggcccg ccgtttttat cgctttcctc gctggtttgc cgctgctgtt gattgccggg
    36181 ctgatccact ggcgtctggg gtggctgaaa gcgtatcaac agaaactggc ttccgctgtg
    36241 ggttccctgc gtaacgatag ccagctcaac acaccaaaag cgatccttat cgacctgatc
    36301 cgtgcactgc cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag
    36361 ctcaacatca gcgaattgct atggtcgttc agcaaaaaac tggcgatttt ctggctggtg
    36421 tttggcctgt gctggaaggt actggagaag aacggcgttg ccgtgcgtca cttcggcatg
    36481 ccggaacagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcattgctg
    36541 cctatccatt tctggtctgt ggtagcagaa ctttccccgc tgcatctgat ggatgatgtg
    36601 ctggggcagg cgatgatttt cttcaacctg ctgctgattg ccttcctggt atggccaatg
    36661 tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactggtcac cattaccgtg
    36721 ctgtcgatta tcccgattgc attgatggtg ctgactgcca cgggttattt ctacaccacg
    36781 ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg tgatcatctg gaacttgctc
    36841 taccagacgg tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg
    36901 ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa
    36961 cccaccattg cactggaaca ggttaaccag cagacgctgc gtattaccat gttgctgatg
    37021 tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc
    37081 tatctcgaca gcattacgct gtggcattac aacggtactg aagctggtgc tgcggtggtg
    37141 aaaaacgtca ccatgggtag tctgttgttt gcgattatcg cctcaatggt ggcctgggcg
    37201 ttgattcgca acctgcctgg tttgctggaa gtgctggtgc tctcgcgact gaatatgcgc
    37261 cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc tgttggtgcg
    37321 atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct ggcagcagca
    37381 ttatccgtag gtcttggttt tggtttacaa gaaattttcg gtaacttcgt ctccggtttg
    37441 atcattctat tcgaacgtcc ggtgcgtatt ggcgatacgg taaccattgg tagcttctcg
    37501 gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa
    37561 gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac
    37621 actactacgc gtctggtgat ccgtatcggc gtggcctatg gctccgatct ggaaaaagtg
    37681 cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg tgatgcacga accaatgccg
    37741 gaagtcttct ttacggcatt tggtgccagc acgttggatc atgagctgcg tctgtatgtg
    37801 cgtgaactgc gtgaccgtag tcgtactgtc gatgagctga accgtactat cgatcagctg
    37861 tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag
    37921 aagggcgatg aggtgacgga agtaaaacgc gactacaaag gcgacgatcc gacaccagcg
    37981 gtagggtaat aacgaaaggg ggcgacattt tgttgccccg agattgctaa caaagtgcgc
    38041 gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caaaaattca
    38101 atatattgca ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
    38161 cccgcaacct taggggacat ttagccaccc catttatttc tcacttttcc gcctcatcat
    38221 cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc cagcgcgcgc agcacggtgc
    38281 gggctgggat ctgattttct tccagcagca caattaaatc gacggccagt ttgacatcgt
    38341 cgggggcatt ttccagtgac atactctctc cattgctagc gggttaaacg cgctaacctg
    38401 ttttcgattt tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact
    38461 tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc gcgccaggcg ggctctgcgt
    38521 tcggcaacca tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc
    38581 cagcgggcaa ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc ggaggcttca
    38641 cgcgcgatgg cttccagttg tgccgccaga tgttccgcca gccaggccac ttgcggcagc
    38701 tgttgctgct caactgcatg acgaagcgca gccagattat cgcccgcctc gtcgagacaa
    38761 gcctgtagtg ttgtcgcacg agtctgaaaa agatgcctgt cgaaacgagc acttagcgtg
    38821 gcgaactgtg acaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt
    38881 tccagcagca gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa
    38941 agtagccact ctgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg
    39001 gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc
    39061 cgtttatcct gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt
    39121 tgctgtaccg ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc
    39181 cgcgcggtgt caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt
    39241 ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt
    39301 tctatatgtg gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacaa
    39361 tcgcagttgc aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag
    39421 gcggtcaaag gttaaacaac tgttactttt gatacgttta aaacgcgccg tgagtaccac
    39481 cgtaacaagc aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa
    39541 tagcatcaaa agcattcagg actacccaaa acccggcatt cttttccgcg atgtcaccag
    39601 cttactggaa gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa
    39661 aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc
    39721 ggtagctctg ggtctgggcg ttggctttgt gccggtccgt aaaccgggca aactgccgcg
    39781 tgaaaccatc agtgaaactt acgacctgga atacggcacc gatcagctgg agatccacgt
    39841 tgatgccatc aaaccgggtg acaaagttct ggtggtggac gatctgctgg caaccggcgg
    39901 cactatcgaa gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc
    39961 gttcattatc aacctgttcg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac
    40021 cagctacagc cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg
    40081 ctacggacag cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt
    40141 accccttcgt gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtctta
    40201 gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc
    40261 gcactggcga acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggtacc
    40321 cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc
    40381 ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc
    40441 tttgtcgatc tgattgaaat cgacgctgcc tcgcgcacca aagttgaaga cactcgcgac
    40501 ctgctggata acgtccagta cgctccggcg cgtggtcgtt tcaaagttta tctcatcgac
    40561 gaagtgcata tgctgtcgcg tcacagcttt aacgcactgt taaaaactct tgaagagccg
    40621 ccggagcacg ttaagtttct gctggcgacg accgatccac agaaattgcc ggtgacgatt
    40681 ttgtcgcgct gtttgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgtcatcaa
    40741 cttgagcaca tcctcaatga agaacatatc gctcacgagc cgcgggcgct gcaattgctg
    40801 gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc tgaccgacca ggcgattgcc
    40861 agcggtgacg gccaggtttc aacccaggcg gtcagtgcga tgctgggtac gcttgacgac
    40921 gatcaggcgc tgtcgctggt tgaagcgatg gtcgaggcca acggcgagcg cgtaatggcg
    40981 ctgattaatg aagccgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc
    41041 ggcctgttgc atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg caacgacatg
    41101 gccgccatcg agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag
    41161 ctttactatc agacgctgtt gattggtcgc aaagaattac cgtatgcgcc ggaccgccgc
    41221 atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc atccgcgtat gccgctgcct
    41281 gagccagaag tgccacgcca gtcctttgca cccgtcgcgc caacggcagt aatgacgcca
    41341 acccaggtgc cgccgcaacc gcaatcagcg ccgcagcagg caccgactgt accgctcccg
    41401 gaaaccacca gccaggtgct ggcggcgcgc cagcagttgc agcgcgtgca gggagcaacc
    41461 aaagcaaaaa agagtgaacc ggcagccgct acccgcgcgc ggccggtgaa taacgctgcg
    41521 ctggaaagac tggcttcggt caccgatcgc gttcaggcgc gtccggtgcc atcggcgctg
    41581 gaaaaagcgc cagccaaaaa agaagcgtat cgctggaagg cgaccactcc ggtgatgcag
    41641 caaaaagaag tggtcgccac gccgaaggcg ctgaaaaaag cgctggaaca tgaaaaaacg
    41701 ccggaactgg cggcgaagct agcggcagaa gccattgagc gcgacccgtg ggcggcacag
    41761 gtgagccaac tttcgctacc aaaactggtc gaacaggtgg cgttaaatgc ctggaaagag
    41821 gagagcgaca acgcagtatg tctgcatttg cgctcctctc agcggcattt gaacaaccgc
    41881 ggtgcacagc aaaaactggc tgaagcgttg agcatgttaa aaggttcaac ggttgaactg
    41941 actatcgttg aagatgataa tcccgcggtg cgtacgccgc tggagtggcg tcaggcgata
    42001 tacgaagaaa aacttgcgca ggcgcgcgag tccattattg cggataataa tattcagacc
    42061 ctgcgtcggt ttttcgatgc ggagctggat gaagaaagta tccgccccat ttgatcgtaa
    42121 gcacagctta cgttcgtcat ccttaacgtg attgagagag aaacctatgt ttggtaaagg
    42181 cggtctgggt aacctgatga agcaagccca gcagatgcaa gaaaaaatgc agaaaatgca
    42241 ggaagagatc gcgcagctgg aagtcaccgg cgaatctggc gcaggtctgg taaaagtgac
    42301 catcaacggt gcacacaact gccgtcgcgt agagatcgac ccgagcctgc tggaagacga
    42361 caaagagatg ctggaagacc tggtggctgc agcattcaac gacgcagcac gtcgtattga
    42421 agaaacgcag aaagaaaaaa tggcctctgt atcctccgga atgcagctgc cgcctggctt
    42481 taagatgccg ttctgatgca aaccagcccg ctgttaacac agcttatgga agcactgcgc
    42541 tgtctgccgg gcgttggccc gaagtcggcg cagcgtatgg cgttcacgct gcttcagcgc
    42601 gatcgtagcg gcgggatgcg tctggcgcag gcgctcaccc gggcgatgtc ggaaatcggc
    42661 cactgcgccg attgccgcac cttcaccgaa caggaagtct gtaacatctg ttcgaatccg
    42721 cgtcgtcagg aaaacggtca aatctgcgtg gtggagagtc cggcggacat ttacgccatt
    42781 gagcagacgg ggcagttttc aggtcgttat tttgtgttga tgggacatct gtcaccgctg
    42841 gacggcatcg gtccggatga tatcggcctt gatcgtctgg aacagcgtct ggcagaggaa
    42901 aaaatcactg aagtgatcct cgccaccaac cctacggttg aaggtgaagc taccgctaac
    42961 tacattgccg agctttgcgc gcaatatgac gtggaagcca gccgaatcgc tcatggcgta
    43021 ccggttggcg gcgaactgga aatggtcgat ggcaccacgc tgtcacactc ccttgccggg
    43081 cgtcataaga ttcgttttta agcaaacgag agcaggatca cctgctctcg cttgaaatta
    43141 ttctcccttg tccccatctc tccctcatcc tgtttttaac cttaaaatgg cattattgag
    43201 gtagacctac atgaaaggac aagaaactcg tggttttcag tcagaagtga aacagcttct
    43261 gcacctgatg atccattctc tctattccaa taaagaaatc ttcctgcgtg agcttatctc
    43321 taacgcctcc gatgcggcgg acaagttgcg tttccgtgcg ctctctaacc cggacctgta
    43381 cgaaggtgat ggcgaactgc gcgttcgtgt ctctttcgat aaagacaagc gcacgctgac
    43441 catctccgat aacggcgtgg ggatgacccg cgacgaagtg attgaccatc tggggactat
    43501 cgctaaatcc ggtaccaaat cattcctcga atccctgggt tctgaccagg cgaaagacag
    43561 ccagctgatc ggtcagtttg gtgttggttt ctactctgcg tttatcgtgg ccgacaaagt
    43621 gaccgtgcgt actcgcgcgg caggcgaaaa accagaaaat ggcgtgttct gggaatcggc
    43681 tggcgaaggt gaatacaccg ttgccgacat caccaaagaa gatcgtggta ctgaaatcac
    43741 cctgcatctg cgtgaaggcg aagacgagtt cctcgatgac tggcgcgtgc gttccatcat
    43801 cagcaaatac tccgaccata tcgcgctgcc ggtagagatc gaaaaacgcg aagagaaaga
    43861 tggcgaaacc gttatctcct gggaaaaaat caacaaagcg caggcgctgt ggactcgtaa
    43921 caagtcggaa atcaccgatg aagagtataa agagttctac aaacacatcg cccacgactt
    43981 taacgatccg ctgacctgga gccacaaccg tgttgaaggt aagcaggagt acaccagcct
    44041 gctgtacatt ccgtcccagg ctccgtggga tatgtggaac cgcgatcata aacacggcct
    44101 gaaactgtat gttcagcgtg tgttcatcat ggacgacgct gaacagttta tgccgaacta
    44161 tctgcgcttc gtgcgtggtc tgattgactc cagcgatctg ccgctgaacg tttcccgtga
    44221 aatcctccag gacagcacgg taacgcgtaa cctgcgcaat gcgctgacca agcgtgtgct
    44281 gcaaatgctg gaaaaactgg cgaaagacga cgcggaaaaa taccagacct tctggcaaca
    44341 gtttggcctg gtactgaaag aaggtccggc ggaagatttc gctaaccagg aagcgatcgc
    44401 caaactgctg cgttttgctt ctactcatac cgattcttct gcgcagaccg tgtctctgga
    44461 agactacgtt tcccgcatga aagaagggca ggagaaaatc tactacatca ctgcagacag
    44521 ctatgcggca gcgaagagca gcccgcacct ggaactgctg cgtaagaaag gcatcgaagt
    44581 tctgctgctt tccgaccgca tcgatgagtg gatgatgaac tatctgactg agttcgacgg
    44641 taaaccgttc cagtcggtgt ctaaagttga cgagtcgctg gaaaaactgg ctgacgaagt
    44701 tgatgagagc gcgaaagaag cggagaaagc actgactccg ttcatcgacc gtgtgaaagc
    44761 cctgctcggc gagcgcgtga aagatgtccg tctgactcac cgtctgaccg atacgccagc
    44821 gatcgtttcg accgacgcgg acgaaatgag cactcagatg gcgaaactgt ttgccgcagc
    44881 gggccagaaa gtgccggaag tgaaatacat ctttgaactg aacccggatc acgtactggt
    44941 gaaacgtgcg gcagatactg aagatgaagc gaagttcagc gagtgggtag aactgctgct
    45001 ggatcaggcg ctgctggcag aacgcggcac gctggaagat ccgaacctgt ttattcgtcg
    45061 tatgaaccag ctgctggttt cctgatgtaa tgccggatga ccttcgtgtc atccggcatt
    45121 tttcttttca tcatctgcac tttccgcaaa ttatctcgcc attaaccgtt tcagcctcag
    45181 gtgcctttct tgaggcaatc gcctgttggt ggtatcgttt atcgcttttt caaaaaaatc
    45241 gacacatttt aaggggattt tcgcaatgcg tatcattctg cttggcgctc cgggcgcggg
    45301 gaaagggact caggctcagt tcatcatgga gaaatatggt attccgcaaa tctccactgg
    45361 cgatatgctg cgtgctgcgg tcaaatctgg ctccgagctg ggtaaacaag caaaagacat
    45421 tatggatgct ggcaaactgg ttaccgacga actggtgatc gcgctggtta aagagcgcat
    45481 tgctcaggaa gactgccgta atggtttcct gttggacggc ttcccgcgta ccattccgca
    45541 ggcagacgcg atgaaagaag cgggcatcaa tgttgattac gttctggaat tcgacgtacc
    45601 ggacgaactg attgttgatc gtatcgtagg ccgccgcgtt catgcgccgt ctggtcgtgt
    45661 ttatcacgtt aaattcaatc cgccgaaagt agaaggcaaa gacgacgtta ccggtgaaga
    45721 actgactacc cgtaaagacg atcaggaaga gaccgtacgt aaacgtctgg ttgaatacca
    45781 tcagatgact gcaccgctga tcggctacta ctccaaagaa gcggaagcgg gtaataccaa
    45841 atacgcgaaa gttgacggca ccaagccggt tgctgaagtt cgcgctgatc tggaaaaaat
    45901 cctcggctaa tcaagccgca gcagatctgc tttcgggcag gtctgctcat ccacctcagg
    45961 caatacctct cacagcaatt agttcttctt cctcactttt ccgctacaat tatcaacaag
    46021 ttgaatcgat aagaggcggt aatgcgtcag actaaaaccg gtatcctgct ggcaaacctg
    46081 ggtacgcccg atgcccccac acctgaagcg gtaaaacgct atctgaaaca atttttaagc
    46141 gacagacgcg tggttgatac ctcacggttg ttatggtggc cattgctgcg cggcgtgatt
    46201 ttgccgctgc gctcgccgcg tgtggcgaag ctgtatgcct ctgtctggat ggaaggtggc
    46261 tcgccgctga tggtttacag ccgccagcaa cagcaggcgc tggcacaacg tttaccggag
    46321 acgcccgtag cgctgggaat gagctacggc tcgccatcac tggaaagcgc cgtagatgaa
    46381 ctcctggcag agcatgtaga tcatattgtg gtgctgccgc tttatccgca attctcctgt
    46441 tctacggtcg gtgcggtatg ggatgaactg gcacgcattc tggcgcgcaa acgtagcatt
    46501 ccggggatat cgtttatacg tgattacgcc gataaccacg attacattaa tgcactggcg
    46561 aacagcgtac gcgcttcttt tgccaaacat ggcgaaccgg atctgctgct gctctcttat
    46621 catggcattc cccagcgtta tgcagatgaa ggcgatgatt acccgcaacg ttgccgcaca
    46681 acgactcgtg aactggcttc cgcattgggg atggcaccgg aaaaagtgat gatgaccttt
    46741 cagtcgcgct ttggtcggga accctggctg atgccttata ccgacgaaac gctgaaaatg
    46801 ctcggagaaa aaggcgtagg tcatattcag gtgatgtgcc cgggctttgc tgcggattgt
    46861 ctggagacgc tggaagagat tgccgagcaa aaccgtgagg tcttcctcgg tgccggcggg
    46921 aaaaaatatg aatatattcc ggcgcttaat gccacgccgg aacatatcga aatgatggct
    46981 aatcttgttg ccgcgtatcg ctaaagctga gcggtaaaga actgagcgcc gtcgcgaaga
    47041 gcctcgtcgg cggttttcat catccgtgaa taatgcaaaa aggcgtgcag cgtgcctggg
    47101 tagagtttga actcacaggg ctgctgatgc gccgctaacg tctggtaaag cagacggctg
    47161 tcatccagca gcggatcgaa ctccgccccg gcaataaaac agggcggaac ttcgcgagtg
    47221 agatcattat taaacagaca gtaatacggc gactcgcggt ccgcgtcgtt gcttaaatac
    47281 gcctcttcat acatctgcaa atcctgttgc gttaagccat cccagacacc gcccaacaga
    47341 cgacgagtca cggaatcccg taatccgtaa agcccatacc acagcaaaac acccgcaatt
    47401 ttaccgcaat cgatctgttt atcacgcaac cacaacgcac tggcgagcgc cagcatggca
    47461 cctgcggaat caccggcaaa gccaatgcgg gacatattaa tttgataatc ctccgcctgc
    47521 tggtggaaat aacaacaagc agccacaatt tcctctatcg cttgcggaaa acgcgcttca
    47581 ggtgaaaggg tgtaatcaat accaatcacc gtacattggc tgtagcttgc cagcaggcgc
    47641 atgatgcgat cgtgggtatc gagattgccg agaataaaac cgcctccatg caaataaaat
    47701 agcgtcgctg ggctatctgg ctgcggacaa aagagacgtg tttccacctg cccatatttt
    47761 gttggaacca tgtaagctct ggttgccatt tctggagcgc ccgcattcca gaatcggcgc
    47821 tcaagcgtgt aatactgtcg ttgctcagca atcgttcccg ttgcgggcca gggcggtaaa
    47881 tccggctgaa gagtattcac aacggtcttc atttcagcag aaataaggtc cagaacaggt
    47941 aatttgtttt ccggcttcat acaaaaactc ctttcaaatt acgtcattgt aaggaaccac
    48001 tgccatgaaa atgcgatccc gcctgctgat attgaaattg gctgcgtctc gcgcgctccc
    48061 gtcagattgt gttaacattc gccgctcagt taaccacccg taaaaacaac catgaaattt
    48121 cccggtaaac gtaaatccaa acattacttc cccgtaaatg cacgcgatcc gctgcttcag
    48181 caattccagc cagaaaacga aaccagcgcc gcctgggtag tgggtatcga tcaaacgctg
    48241 gtcgatattg aagcgaaagt ggatgatgaa ttcattgagc gttatggatt aagcgccggg
    48301 cattcactgg tgattgagga tgatgtagcc gaagcgcttt atcaggaact aaaacagaaa
    48361 aacctgatta cccatcagtt tgcgggtggc accattggta acaccatgca taactactcg
    48421 gtgctcgcgg acgaccgttc ggtgctgctg ggcgtcatgt gcagcaatat tgaaattggc
    48481 agctatgcct atcgttacct gtgtaacacc tccagccgta ccgatcttaa ctatctacaa
    48541 ggcgtggatg gcccgattgg tcgttgcttt acgctgattg gcgagtccgg ggaacgtacc
    48601 tttgctatca gccctggcca catgaaccag ctgcgggctg aaagtattcc ggaagatgtg
    48661 attgccggag cctcgactct ggttctcacc tcttatctgg tgcgttgcaa gccgggtgaa
    48721 cccatgccgg aagcaaccat gaaagccatt gagtacgcga agaaatataa cgtaccggtg
    48781 gtgctgacgc tgggaactaa gtttgtcatt gcagagaatc cgcagtggtg gcagcaattc
    48841 ctcaaagacc acgtctctat ccttgcgatg aacgaagatg aagccgaagc gttgaccgga
    48901 gaaagcgatc cgttgttggc atctgacaag gcgctggact gggtagatct ggtgctgtgc
    48961 accgccgggc caatcggctt gtatatggcg ggctttaccg aagacgaagc gaaacgtaaa
    49021 acccagcatc cgctgctgcc gggcgctata gcggaattca accagtatga gtttagccgc
    49081 gccatgcgcc acaaggattg ccagaatccg ctgcgtgtat attcgcacat tgcgccgtac
    49141 atgggcgggc cggaaaaaat catgaacact aatggagcgg gggatggcgc actggcagcg
    49201 ttgctgcatg acattaccgc caacagctac caccgtagca acgtaccaaa ctccagcaaa
    49261 cataaattca cctggttaac ttattcatcg ttagcgcagg tgtgtaaata tgctaaccgt
    49321 gtgagctatc aggtactgaa ccagcattca cctcgtttaa cgcgcggctt gccggagcgt
    49381 gaagacagcc tggaagagtc ttactgggat cgttaagtta tcgtcggttc gtaggccaga
    49441 taaggcgttc acgccgcatc tggcattttg ctctcaatgc ctgatgcgac gctggcgcgt
    49501 cttatcaggc ctacatatcc gcgcctatta tccggcaacc accgtttacc ccgtcaccac
    49561 ctcacccgcc ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgctcacc
    49621 catcactacc tgattcgcgc cacgttcggt gatatacgcc acttcatcgt cataatgggc
    49681 gcgggcaata atctcaatat ccggattttt cgcgcgggca gatgccacaa tctcgcctgc
    49741 ttcataaccg ttgggaatcg tcaggatcag ccattttgca cattccagat gcgccagttg
    49801 cataatttct tcgttcgccg cattgcccaa tactgcgcgg acaccgcgct ctcgcagctc
    49861 atcaacacgg gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt
    49921 ctcccccagc aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc
    49981 cactgggatc tgcttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc
    50041 ggtcttcgcc agatatttct ccagcagcgc aaacaggacc gggttgagca taatcgacaa
    50101 aatcgcacct gccagtacca ggttctgccc ggcttgcggc aacagatcca gcgccatgcc
    50161 cagtcccgcc aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatggttaa
    50221 tgccgtgcgc tgggagtgac caaacagtcg caccaggaaa aatgcggcta acgacttacc
    50281 aaacagaata atcgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg
    50341 atcaaacaac atcccgacgg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag
    50401 cgtatcgtgg gcggcacggt gactcagttc agactcgttc agtaccatcc cggcaaagaa
    50461 cgcaccgagt gcaaaggaga catcaaacag ctccaccgca ccaaaggcaa tccctaacgc
    50521 cagcgccagc accgacaggg taaacagctc gcgagaaccg gttgccgcgc tgcgtgccat
    50581 aatccacggc accagacggc gacctaccag catcataatg gcgataaacg cgatcacttt
    50641 gccgatggtg atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat
    50701 cattcctgcc actgcgggca gcaacaccag cgtcagaacc atcaccaggt cttccacaat
    50761 caaccaaccg atggcgattt gcccacgctg actgtcaatt aattgccgtt cttcaagtgc
    50821 gcgcagtaac accacggtac tggcggtgga aagacataaa ccgaacacga taccggtcat
    50881 taacgaccag cccagcacgg cagagagcgc catacccagc agcgtcgcca cggctatctg
    50941 ggcgatcgca ccgggaatgg cgatggcctt taccgccatc aaatccttca gcgaaaagtg
    51001 cagacccacg ccaaacatca acagaatgac gcccagttca gccagttccg gcgcaagctt
    51061 ggtatcggca acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc
    51121 caccagagga gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag
    51181 gccgccaaca atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcctgg
    51241 tggttattgt ccatttttgg ccgggaaaac caaaattaca ggtaatagtt tatgacaatt
    51301 tcattgatga tgttcatgaa taattattga attttgcaga aaaatggaat tagctgcaaa
    51361 aaaagcacgg atcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta
    51421 aaacgatgca gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc
    51481 aggcaggaat atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa
    51541 ctcgatgctg gtgtgatcgg cgataagccc cagaactgcc gctcccagac ctcccatgcc
    51601 aaaagcaaaa ccgaaaaaga gtccagaaac cataccgata cgtcccggaa gcagctcctg
    51661 agcgtaaacc agaatggcag agaatgccga agcgaggata aatccaataa tcaccgttaa
    51721 aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc gtaaacggcg caacgccgag
    51781 gatagagccc caaatcacat atttccgccc aattttatcc cctacaggcc cgccgatcac
    51841 cgtacctgcc gcaacggcaa acaggaaggc aaacagatga agctgagcat tctggataga
    51901 taatccgaat ttttgcatca gataaaaggt gtaatagctg ctgatgctcg ccatatagaa
    51961 atatttcgag aaaatgagga ttaacagaat gctgaccgcc agtaccactt tgttgcgcgg
    52021 cagtggattg ataatcgtcg ctttgggttt tcctttattc attcggtgct gtgccgagta
    52081 ccaacggctg atttgcgcca acaccacgat cgccagcagt gccgcaagca caaaccaggc
    52141 aacgttgcct ttgccataag gcgcgataat caccgccgcc agcaaaggtc ccagggaact
    52201 gccaaagttg ccgccgacct gaaagataga ttgtgccagg ccatgccgcc cgccggaagc
    52261 catacgggcc acgcgagaag attccggatg aaagaccgat gaaccggtac cgaccagcgc
    52321 cgccgccagc agaactgcgc caaaactgtc cgccagcgca agcagcacca gaccacttaa
    52381 ggtaaagcac atgccaattg gcaacgacca cggcatcgga tatttatcgg tccagtagcc
    52441 gaccactggt tgcagtagcg aagaggcgag ctggaaggtg agggttatca tgccaatctg
    52501 cataaatgtc agagaaaatt ctgactgaag cagaggataa atcgccagaa tcagcgattg
    52561 gatcatgtcg ttcagcagat gtgagaggct gatagcacct aaaataccaa acgatgttcg
    52621 ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac tcattgccat
    52681 aggaaagtca ctttttcagg gttgcgatgt aaagaatgat cttatttgtg attattaccc
    52741 gactaacata cctgtatgcg ccgcctgaag gaagtctcaa cgctgaatac agaatttcta
    52801 atctggatgc agatttatct tcaccggacg cagacttgtc tatgatgtcg cgtcatacta
    52861 tttttcaaca cgttgaaatc aggtcaggga gagaagtatg aaattattgc agcggggcgt
    52921 ggcgttagca ctgttaacca catttacact ggcgagtgaa gctgctctgg cgtatgagca
    52981 ggataaaacc tacaaaatta cagttctgca taccaatgat catcatgggc atttttggcg
    53041 caatgaatat ggcgaatatg gtctggcggc tcaaaaaacg ctggtggatg gtatccgcaa
    53101 agaggttgcg gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca ttaacactgg
    53161 cgtgcccgag tctgacttac aggatgccga acctgatttt cgcggtatga atctggtggg
    53221 ctatgacgcg atggcgatcg gtaatcatga atttgataat ccgctcaccg tattacgtca
    53281 gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat atctaccaga aaagtaccgg
    53341 cgagcgcctg tttaaaccat gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat
    53401 tggtctgacg acggatgaca cagcgaagtt aggcaaccca gaaaacttca ccgatattga
    53461 gttccgtaag cctgccgatg aagcgaagct ggtgattcag gagctgcaac agacagaaaa
    53521 gccagacatt attatcgcgg cgacccatat ggggcattac gacaatggtg agcacggctc
    53581 taatgcaccg ggcgatgtgg agatggcacg cgtgctgcct gccggatcgc tggcgatgat
    53641 cgtcggtggt cactcgcaag atccggtctg catggcggca gaaaacaaaa aacaggtcga
    53701 ttacgtgccg ggtacgccat gcaaaccgga tcaacaaaac ggcatctgga ttgtgcaggc
    53761 acatgagtgg ggcaaatacg tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa
    53821 aatggttaac taccagctga ttccggtgaa cctgaagaag aaagtgacct gggaagacgg
    53881 gaaaagcgag cgcgtgctgt acacccctga aatcgctgaa aaccagcaaa tgatctcgct
    53941 gttatcgccg ttccagaaca aaggcaaagc gcagctggaa gtgaaaatag gcgaaaccaa
    54001 tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta cagaccaata tggggcggtt
    54061 aattctggca gcacaaatgg atcgcactgg tgccgacttt gcggtgatga gcggaggcgg
    54121 aattcgtgat tctatcgaag caggcgatat cagctataaa aacgtgctga aagtgcagcc
    54181 attcggcaat gtggtggtgt atgccgacat gaccggtaaa gaggtgattg attacctgac
    54241 cgccgtcgcg cagatgaagc cagattcagg tgcctacccg caatttgcca acgttagctt
    54301 tgtggcgaaa gacggcaaac tgaacgacct taaaatcaaa ggcgaaccgg tcgatccagc
    54361 gaaaacttac cgtatggcga cattaaactt caatgccacc ggcggtgatg gatatccgcg
    54421 ccttgataac aaaccgggct atgtgaatac cggctttatt gatgccgaag tgctgaaagc
    54481 gtatatccag aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag
    54541 ctggcagtaa tccgaaagtg ccggatgttc gcatccggca caatgcttaa tcgcggcggg
    54601 cgatatcagc aaatttggca tcgaggatct tcgccagatc gcctgccgcc agttcgatat
    54661 ccagtccgcg cttgccgccg gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga
    54721 taatcgttgg cagacgtttt ttctgcccca gtgggctaat ccccccaacc aggtatcccg
    54781 tcgaacgctg cgcgaccatc ggatcggcca tctcaacttt cttggcaccc agcgcttttg
    54841 ctactttttt aaggtccagt tgacctgcga ccggcgtaac ggccacggca aggtgtttca
    54901 tatcaccgtt cactgccacc agcagcgttt tgtagacctg atccggattc aaacctaatt
    54961 ttttgacgac ttcatcgcca aaattggttt cagccggatc gtgctcgtag gtatggattt
    55021 gaaacgaaat cttgtttttt tcgagtaatt taactgcggg tgtcatggta attttcctgc
    55081 cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct
    55141 tgcctaagag tattggcagg atggtgagat tgagcgacaa tcgagttaca ccgtcgctta
    55201 aagtgacggc ataataataa aaaaatgaaa ttcctctttg acgggccaat agcgatattg
    55261 gccatttttt tagcgcaaca tttgcggtaa attcccttct ccatacaggt gtagtgcacc
    55321 gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt
    55381 tcgctgatgc atcagcacat cgtacagcga ctgactgaac gtattgggca gcgttatttc
    55441 attattttgc ggcggcgcat tcagccacca gctcatcatt tgttgcagca accgtgcgtt
    55501 ggtatgccag tgggtcagcg tatcgtccag cagtgccagt cctttgtcag ggagctggag
    55561 caacatggta atctggtttt cagccccttc cagttcaatc acgggtttat gttgttgctt
    55621 cgccgcctgc aataactggt aatcgatacc gtattctgcc cgcagcccca gtttttgtgc
    55681 ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt tgggtagaaa agagtgacgg
    55741 agaaatgccc atcacctggc taatgtgctg cagattttgt aattgctcct cgctaatacg
    55801 ctcttccagc gcctcgcagg caggcaaatt agcaaaaggc gtatcgctgg tggaaacatc
    55861 cgcctcgacg atcagcgcat cggcgttttt gagctttttg agcaaacggg cgggcagggg
    55921 agccatatcg tggctaccca tatgaatact gccaatcaga tgaaaatggc gattgccggg
    55981 gagggtgata tcgatggcag gccaggagta atgattaccg cgcagcgcgg cccaaagcgt
    56041 ttttacccgg tacaacagat ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg
    56101 gggagggatg gcgcaaatgg agttgggaac ttgtttaatg tgtttgtatg attcagtatg
    56161 ttcttgtatc actattcaca gggaagcaac agttaaaaac tatgaaacag gcaacaagaa
    56221 aaccgacgac acccggagat attcttctct atgaatatct ggaaccgcta gatttgaaaa
    56281 tcaatgagtt agcagagttg ctgcatgttc atcgtaatag catcagtgca ctgatcaata
    56341 acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag
    56401 tcgatttttg gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc
    56461 gtacctagga agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag
    56521 agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc atctcgtagg ccggataagg
    56581 cgttcacgcc gcatccggca tccgagcacc attgcctgat gcgacgctta cgcgtcttat
    56641 caggcctaca aacctgtgcc tgaaccgtag gccggataag gcgttcacgc cgcatccggc
    56701 agtcatgcgt cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta
    56761 ttccttcggt ttaaaccgca gcaaccggtt ggcgttactc actacggtaa tcgacgagag
    56821 cgccattgcc gctccggcaa ccaccgggtt aagcagtgtt ccagtaaacg gccacaaaat
    56881 accggcggca accggaatac cgatgctgtt gtagataaac gcgccgagca ggttctgctt
    56941 catgttgcgc agcgttgcgc gggaaatagc gagcgcatcc gcaacgccca tcaggctatg
    57001 gcgcatcagg gtaatcgccg cggtttcaat agcaacatca ctgccgccac ccatcgcaat
    57061 gccgacatcc gcctgagcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac
    57121 ctgacgtcct tcactttgca gacgtttgat cgcttcggct ttaccgtctg gcagcacccc
    57181 ggcgatcacc tcatcaatcc ctgcttcttt ggcgatcgca ttggcggtgg ttgggttatc
    57241 cccggtcaac atcaccagac gatatcccgc tttatgcaga cgttgcagcg ccgccacgct
    57301 atcactacgc aacggatcgc gtacagccag cagggctacc gctttcccgt caaccgccag
    57361 cagcacaggc gttgctcctt gcgatgcctg agctgaaata tcggcttcaa tcgctttagt
    57421 atcgacttgc tgatcattta acaacgactg attgcccagc aataacgcat gaccttcagc
    57481 ttcaccgctc acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gcagctgcat
    57541 atcgcttgct ttatcgagga tcgcccgtgc cagcggatgg ctggaacctt gttccagtgc
    57601 cgccgccaga cgcagcgcct gcgcttcgtc aaaatcagca aatgttttca ctgcgacaac
    57661 ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact
    57721 ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac
    57781 gccggaaata atcgacatcg gcgtcgccag ccccagcgca cacggacagg caataatcag
    57841 caccgtggtg gcaatcacca gggtatagac aatctgcggt gccggaccaa agaaatacca
    57901 gattgccgca ctgacaagcg caataaccac tactaccggc acaaatacgg ctgagatttt
    57961 atccgccagc tgaccgattt ctggcttact gctctgggcc tggcgcacca tacgaatgat
    58021 ccttgatagc gtggtatggc taccaaccgc actggcgcga aacagcacgc taccgtcctg
    58081 taccactgta ccggcatgga cgctatcgcc ttcgcctttt tgctgcggaa taggttcgcc
    58141 cgtcagcatc gcttcatcca gccatgcttc gccctgggta atctcgccat ctactggcac
    58201 gcggtcgccc gtcgtcaggc gcagtagcat gcctggctga acttccgcca gcggcacgct
    58261 tttttcacct tcgtcagtaa ccaggcgcgc cgtcggcgga gttaaatcga gtaatttctc
    58321 cagcgccttt gaagaacgct gacgtgcgcg cgcttccagc atatggccga gattaatcag
    58381 accgataatc atcgcgctgg cttcgtaata aagatgtcgc gcttccatcg ggaaccattg
    58441 cggccacagg ttgacgctca tcgaatagag ccacgccacg ccagtaccca gcgccaccag
    58501 cgtatccatc gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc
    58561 gccggcgaaa accatcactg ccagggttat caggccgata actaaccaca agctgcggtt
    58621 gtcagcggtg accatcatgt tatcgccgat catcccccag accatcaccg ggatacccac
    58681 cgccagtgcg acaattgcct gccagcggaa gcgcttcatc gtagcgacgg cggtttcttg
    58741 ctggcgttcg cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc
    58801 caccgcctgc actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc
    58861 caggtttacc cgtgcctgag tgacgcccgg tacgctttgc aacgcatttt gtacgcgggt
    58921 gacacagctg gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc
    58981 ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc
    59041 cgccagcggt ttagcctttg ggtggcttac agatacgtca taacccgctt gtttgatggt
    59101 ttcgatcagc tgttctgcac tggctgtccc ggtaacgtgc gcttcagtga tagacacatc
    59161 cgcttgttcc acatccgggc gctgttcgag actttctttc acgcgtttaa cgcagtgacc
    59221 gcaggacagg ccgtccaggg tcaggtcgat agtttgtgac ataaaacact cctttaagac
    59281 agttttgact ggctgtgata aaggttaaac cttccagcaa ggggaaggtc aagaaattaa
    59341 taacccaggc gagtaaaagt ccgtaaagat taaaaaatcg gctcgatttg catcaggatt
    59401 agacatttat ctctttgttt tcctgtagtt aagttgcggg tgctaagtta aatcaggatg
    59461 cctgaaaatc ggcaccgggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa
    59521 aagatgttag atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac
    59581 tcacttaacg gcggacaaaa tgccgattac attccctttc tggcgaatgt gcctggtcaa
    59641 ctggtagcag tggctatcgt gaccagcgat ggcaacgtct atagtgcggg tgacagtgat
    59701 taccgctttg cgctggaatc catctcgaaa gtctgtacat tagcccttgc gttagaagat
    59761 gtcggcccgc aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac
    59821 tcagttatcg ccttagagtt gcatggcggc aaaccgcttt cgccactggt aaatgctggc
    59881 gctattgcca ccaccagcct gattaacgct gaaaatgccg aacaacgctg gcagcgaatt
    59941 ttacatatcc aacagcaact ggctggtgag ctggtagcgc tctctgacga agtcaacgag
    60001 tcggaacaaa caaccaactt ccataaccgg gccattgcct ggctgctgta ctccgccgga
    60061 tatctctatt gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctccacgctc
    60121 atcaatactg ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg
    60181 ttgacgcata aacgcgttct tcaggccgac aacgtgccgt acattctggc cgaaatgatg
    60241 atggaagggc tgtatggtcg ctccggtgac tgggcgtatc gtgttggttt accgggcaaa
    60301 agcggtgtag gtggcggtat tctggcggtc gtccctggcg tgatgggaat tgccgcgttc
    60361 tcaccaccgc tggacgaaga aggcaacagt gttcgcggtc aaaaaatggt ggcatcggtc
    60421 gctaagcaac tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata
    60481 acggtaacaa acccctcggt ctatggaatg tcgtttccat cggtattggg gcaatggtgg
    60541 gggcggggat cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc tcgacctggg
    60601 tcgcctttgc ttttggcggt attgtggcga tgttttccgg ttatgcctat gcgcgtctgg
    60661 gggcgagcta tcccagcaat ggcggcatta tcgacttctt tcgtcgcgga ttaggcaacg
    60721 gcgtcttttc gctggcgctc tcgttactgt acctgttgac gctggcggtg agcatcgcca
    60781 tggtcgcccg tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg
    60841 agcaccttat tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct
    60901 taagcaacca tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc
    60961 tgttattgct gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg
    61021 cgccccccag ctccggtgcg ttcttctcct gtatcgggat aaccttcctt gcctatgcgg
    61081 gctttggcat gatggcgaac gcggcggata aagtgaaaga tccgcaggtc attatgccac
    61141 gggcgtttct ggtggcaatt ggcgttacca cgttgcttta tatctcgctg gcgctggttt
    61201 tgcttagcga tgtatcggca ttagaattag aaaaatatgc cgataccgcc gtagcgcagg
    61261 ctgcttctcc gctgctcggg catgtgggtt atgtgatcgt cgtcatcggc gctttactgg
    61321 cgacggcttc agccatcaac gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg
    61381 gcagcgaacg cgaactaccg aagctaatga ataaatccct gtggcggcag agtacctggg
    61441 gcaacatcat tgtcgtggtg ttgattatgc tgatgacggc ggcactaaat ttaggctcac
    61501 tcgccagcgt tgccagcgcc acctttttga tttgctatct ggcggtgttt gtggtggcga
    61561 ttcgcctgcg tcatgatatt cacgcctcgt tgccgattct tatcgtaggt acgttggtga
    61621 tgttgttggt gatcgttggc tttatctaca gcctgtggtc ccagggcagc cgtgcgttga
    61681 tatggattat tggtgcaatc ttactcagcc ttattgtggc aatggtcatg aagcgcaata
    61741 aaaccgtata acacctctct gcgcgcagta ctgcctgtac tgcgcctttg tctttaattc
    61801 aaattacctt tcttattcct ttaataaata agtcacaaaa aaatgatata tacgcgataa
    61861 atctgtgatg ctaatcactt attaccgtgg attattctta atgtgtatga t
//
LOCUS       DAZUGK010000005        58435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZUGK010000005 DAZUGK010000000
VERSION     DAZUGK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.005"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(189..265)
                     /locus_tag="ACHY5W_000242"
     tRNA            complement(189..265)
                     /locus_tag="ACHY5W_000242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Arg,seq:acg)
     gene            complement(269..361)
                     /locus_tag="ACHY5W_000243"
     tRNA            complement(269..361)
                     /locus_tag="ACHY5W_000243"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Ser,seq:gct)
     gene            complement(677..862)
                     /gene="csrA"
                     /locus_tag="ACHY5W_000244"
     CDS             complement(677..862)
                     /gene="csrA"
                     /locus_tag="ACHY5W_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFS4445213.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1097..3727)
                     /gene="alaS"
                     /locus_tag="ACHY5W_000245"
     CDS             complement(1097..3727)
                     /gene="alaS"
                     /locus_tag="ACHY5W_000245"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4445214.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3855..4355)
                     /gene="recX"
                     /locus_tag="ACHY5W_000246"
     CDS             complement(3855..4355)
                     /gene="recX"
                     /locus_tag="ACHY5W_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4445215.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4598..5659)
                     /gene="recA"
                     /locus_tag="ACHY5W_000247"
     CDS             complement(4598..5659)
                     /gene="recA"
                     /locus_tag="ACHY5W_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4445216.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5739..6236)
                     /gene="pncC"
                     /locus_tag="ACHY5W_000248"
     CDS             complement(5739..6236)
                     /gene="pncC"
                     /locus_tag="ACHY5W_000248"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFS4445217.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6381..7466)
                     /gene="mltB"
                     /locus_tag="ACHY5W_000249"
     CDS             complement(6381..7466)
                     /gene="mltB"
                     /locus_tag="ACHY5W_000249"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFS4445218.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7722..8285
                     /gene="srlA"
                     /locus_tag="ACHY5W_000250"
                     /gene_synonym="gutA"
     CDS             7722..8285
                     /gene="srlA"
                     /locus_tag="ACHY5W_000250"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4445219.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8282..9241
                     /gene="srlE"
                     /locus_tag="ACHY5W_000251"
                     /gene_synonym="gutE"
     CDS             8282..9241
                     /gene="srlE"
                     /locus_tag="ACHY5W_000251"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4445220.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGIAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9252..9623
                     /gene="srlB"
                     /locus_tag="ACHY5W_000252"
                     /gene_synonym="gutB"
     CDS             9252..9623
                     /gene="srlB"
                     /locus_tag="ACHY5W_000252"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4445221.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSILKFESVKE"
     gene            9627..10406
                     /gene="srlD"
                     /locus_tag="ACHY5W_000253"
     CDS             9627..10406
                     /gene="srlD"
                     /locus_tag="ACHY5W_000253"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFS4445222.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10512..10871
                     /gene="gutM"
                     /locus_tag="ACHY5W_000254"
     CDS             10512..10871
                     /gene="gutM"
                     /locus_tag="ACHY5W_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4445223.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10938..11711
                     /gene="srlR"
                     /locus_tag="ACHY5W_000255"
                     /gene_synonym="gutR"
     CDS             10938..11711
                     /gene="srlR"
                     /locus_tag="ACHY5W_000255"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFS4445224.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEATVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11704..12669
                     /gene="gutQ"
                     /locus_tag="ACHY5W_000256"
     CDS             11704..12669
                     /gene="gutQ"
                     /locus_tag="ACHY5W_000256"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFS4445225.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(12666..14180)
                     /gene="norR"
                     /locus_tag="ACHY5W_000257"
     CDS             complement(12666..14180)
                     /gene="norR"
                     /locus_tag="ACHY5W_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFS4445226.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14367..15806
                     /gene="norV"
                     /locus_tag="ACHY5W_000258"
     CDS             14367..15806
                     /gene="norV"
                     /locus_tag="ACHY5W_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFS4445227.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15803..16936
                     /gene="norW"
                     /locus_tag="ACHY5W_000259"
     CDS             15803..16936
                     /gene="norW"
                     /locus_tag="ACHY5W_000259"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFS4445228.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17064..19316)
                     /gene="hypF"
                     /locus_tag="ACHY5W_000260"
     CDS             complement(17064..19316)
                     /gene="hypF"
                     /locus_tag="ACHY5W_000260"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFS4445229.1"
                     /translation="MSKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWPQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPASLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMHGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIATA
                     RWLAGEVQNG"
     gene            complement(19469..19996)
                     /gene="hydN"
                     /locus_tag="ACHY5W_000261"
     CDS             complement(19469..19996)
                     /gene="hydN"
                     /locus_tag="ACHY5W_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFS4445230.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20145..21155)
                     /gene="ascG"
                     /locus_tag="ACHY5W_000262"
     CDS             complement(20145..21155)
                     /gene="ascG"
                     /locus_tag="ACHY5W_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFS4445231.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALVQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21416..22873
                     /gene="ascF"
                     /locus_tag="ACHY5W_000263"
     CDS             21416..22873
                     /gene="ascF"
                     /locus_tag="ACHY5W_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFS4445232.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVALRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWGFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEAQKHQSVQPTVAKEVSLN"
     gene            22882..24306
                     /gene="ascB"
                     /locus_tag="ACHY5W_000264"
     CDS             22882..24306
                     /gene="ascB"
                     /locus_tag="ACHY5W_000264"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFS4445233.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNPQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24420..24839)
                     /locus_tag="ACHY5W_000265"
     CDS             complement(24420..24839)
                     /locus_tag="ACHY5W_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4445234.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24836..25147)
                     /locus_tag="ACHY5W_000266"
     CDS             complement(24836..25147)
                     /locus_tag="ACHY5W_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFS4445235.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25309..26064)
                     /locus_tag="ACHY5W_000267"
     CDS             complement(25309..26064)
                     /locus_tag="ACHY5W_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445236.1"
                     /translation="MCAFLLSLVQSALATSFTEYLPMSGSEYAQKRALKPLLTMPYYT
                     EQTWHFRKIGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGLLANMAGKGQLYRAD
                     LDRNGIQDLVIWLPSAGNGLAPYAHLILMTFTHEGRPCVFEPWGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDTQTEDLSLTQRCLIKGDVLDGVNQN"
     gene            complement(26107..26574)
                     /gene="hycI"
                     /locus_tag="ACHY5W_000268"
     CDS             complement(26107..26574)
                     /gene="hycI"
                     /locus_tag="ACHY5W_000268"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFS4445237.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(26567..26977)
                     /gene="hycH"
                     /locus_tag="ACHY5W_000269"
     CDS             complement(26567..26977)
                     /gene="hycH"
                     /locus_tag="ACHY5W_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFS4445238.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26974..27741)
                     /gene="hycG"
                     /locus_tag="ACHY5W_000270"
     CDS             complement(26974..27741)
                     /gene="hycG"
                     /locus_tag="ACHY5W_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFS4445239.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEDQVARWLEAENDPRLNEIVSHLNQVVEEARIR"
     gene            complement(27741..28283)
                     /gene="hycF"
                     /locus_tag="ACHY5W_000271"
     CDS             complement(27741..28283)
                     /gene="hycF"
                     /locus_tag="ACHY5W_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFS4445240.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28293..30002)
                     /gene="hycE"
                     /locus_tag="ACHY5W_000272"
     CDS             complement(28293..30002)
                     /gene="hycE"
                     /locus_tag="ACHY5W_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFS4445241.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTRCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30020..30943)
                     /gene="hycD"
                     /locus_tag="ACHY5W_000273"
     CDS             complement(30020..30943)
                     /gene="hycD"
                     /locus_tag="ACHY5W_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFS4445242.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLQAA"
     gene            complement(30946..32772)
                     /gene="hycC"
                     /locus_tag="ACHY5W_000274"
     CDS             complement(30946..32772)
                     /gene="hycC"
                     /locus_tag="ACHY5W_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFS4445243.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32769..33380)
                     /gene="hycB"
                     /locus_tag="ACHY5W_000275"
     CDS             complement(32769..33380)
                     /gene="hycB"
                     /locus_tag="ACHY5W_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFS4445244.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33505..33966)
                     /gene="hycA"
                     /locus_tag="ACHY5W_000276"
     CDS             complement(33505..33966)
                     /gene="hycA"
                     /locus_tag="ACHY5W_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFS4445245.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34178..34528
                     /gene="hypA"
                     /locus_tag="ACHY5W_000277"
     CDS             34178..34528
                     /gene="hypA"
                     /locus_tag="ACHY5W_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS4445246.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34532..35404
                     /gene="hypB"
                     /locus_tag="ACHY5W_000278"
     CDS             34532..35404
                     /gene="hypB"
                     /locus_tag="ACHY5W_000278"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFS4445247.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35395..35667
                     /gene="hypC"
                     /locus_tag="ACHY5W_000279"
     CDS             35395..35667
                     /gene="hypC"
                     /locus_tag="ACHY5W_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFS4445248.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35667..36788
                     /gene="hypD"
                     /locus_tag="ACHY5W_000280"
     CDS             35667..36788
                     /gene="hypD"
                     /locus_tag="ACHY5W_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFS4445249.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36785..37795
                     /gene="hypE"
                     /locus_tag="ACHY5W_000281"
     CDS             36785..37795
                     /gene="hypE"
                     /locus_tag="ACHY5W_000281"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFS4445250.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37869..39947
                     /gene="flhA"
                     /locus_tag="ACHY5W_000282"
     CDS             37869..39947
                     /gene="flhA"
                     /locus_tag="ACHY5W_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFS4445251.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(39984..40337)
                     /gene="ygbA"
                     /locus_tag="ACHY5W_000283"
     CDS             complement(39984..40337)
                     /gene="ygbA"
                     /locus_tag="ACHY5W_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFS4445252.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEQKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40369..40548)
                     /locus_tag="ACHY5W_000284"
     CDS             complement(40369..40548)
                     /locus_tag="ACHY5W_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445253.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     EPFFFRVIVPFKIKM"
     gene            40624..43185
                     /gene="mutS"
                     /locus_tag="ACHY5W_000285"
     CDS             40624..43185
                     /gene="mutS"
                     /locus_tag="ACHY5W_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4445254.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETIDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43291..43947
                     /gene="pphB"
                     /locus_tag="ACHY5W_000286"
     CDS             43291..43947
                     /gene="pphB"
                     /locus_tag="ACHY5W_000286"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS4445255.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLYQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKGIAEIELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPNSRRLSFYKIK
                     "
     gene            complement(43988..44224)
                     /locus_tag="ACHY5W_000287"
     CDS             complement(43988..44224)
                     /locus_tag="ACHY5W_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFS4445256.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRI
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(44235..45662)
                     /locus_tag="ACHY5W_000288"
                     /pseudo
     CDS             complement(44235..45662)
                     /locus_tag="ACHY5W_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
     gene            complement(45662..46255)
                     /locus_tag="ACHY5W_000289"
     CDS             complement(45662..46255)
                     /locus_tag="ACHY5W_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFS4445257.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGFEHPHARRWQGLPQVRNFSQENE"
     gene            46401..46808
                     /locus_tag="ACHY5W_000290"
     CDS             46401..46808
                     /locus_tag="ACHY5W_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4445258.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(46928..47920)
                     /gene="rpoS"
                     /locus_tag="ACHY5W_000291"
     CDS             complement(46928..47920)
                     /gene="rpoS"
                     /locus_tag="ACHY5W_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFS4445259.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(47983..49077)
                     /gene="nlpD"
                     /locus_tag="ACHY5W_000292"
     CDS             complement(47983..49077)
                     /gene="nlpD"
                     /locus_tag="ACHY5W_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFS4445260.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(49262..49888)
                     /gene="pcm"
                     /locus_tag="ACHY5W_000293"
     CDS             complement(49262..49888)
                     /gene="pcm"
                     /locus_tag="ACHY5W_000293"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFS4445261.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(49882..50643)
                     /gene="surE"
                     /locus_tag="ACHY5W_000294"
     CDS             complement(49882..50643)
                     /gene="surE"
                     /locus_tag="ACHY5W_000294"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFS4445262.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(50624..51673)
                     /gene="truD"
                     /locus_tag="ACHY5W_000295"
     CDS             complement(50624..51673)
                     /gene="truD"
                     /locus_tag="ACHY5W_000295"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFS4445263.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(51670..52149)
                     /gene="ispF"
                     /locus_tag="ACHY5W_000296"
     CDS             complement(51670..52149)
                     /gene="ispF"
                     /locus_tag="ACHY5W_000296"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFS4445264.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(52149..52859)
                     /gene="ispD"
                     /locus_tag="ACHY5W_000297"
     CDS             complement(52149..52859)
                     /gene="ispD"
                     /locus_tag="ACHY5W_000297"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS4445265.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLSLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCMTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(52878..53189)
                     /gene="ftsB"
                     /locus_tag="ACHY5W_000298"
     CDS             complement(52878..53189)
                     /gene="ftsB"
                     /locus_tag="ACHY5W_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFS4445266.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(53383..53706)
                     /gene="ygbE"
                     /locus_tag="ACHY5W_000299"
     CDS             complement(53383..53706)
                     /gene="ygbE"
                     /locus_tag="ACHY5W_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFS4445267.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(53756..54361)
                     /gene="cysC"
                     /locus_tag="ACHY5W_000300"
     CDS             complement(53756..54361)
                     /gene="cysC"
                     /locus_tag="ACHY5W_000300"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFS4445268.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(54361..55788)
                     /gene="cysN"
                     /locus_tag="ACHY5W_000301"
     CDS             complement(54361..55788)
                     /gene="cysN"
                     /locus_tag="ACHY5W_000301"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFS4445269.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLMDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(55790..56698)
                     /gene="cysD"
                     /locus_tag="ACHY5W_000302"
     CDS             complement(55790..56698)
                     /gene="cysD"
                     /locus_tag="ACHY5W_000302"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFS4445270.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            56950..57987
                     /gene="iap"
                     /locus_tag="ACHY5W_000303"
     CDS             56950..57987
                     /gene="iap"
                     /locus_tag="ACHY5W_000303"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFS4445271.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        13773 a        16042 c        15102 g        13518 t
ORIGIN      
        1 ttactttact gcagattttt tgataccgct actaaagccg catcaagtaa gagatggtgc
       61 atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa gcaacgttat
      121 cctccctggt gcctgcggtt attaccgcca cctcttccat accttcatcg ctgaaagtac
      181 gtagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
      241 ctctatccag ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg
      301 cagcttttga ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac
      361 cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg
      421 cacatattac tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc
      481 acacttcacg ctcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc aacagaaaaa
      541 cggaggtctg acagatagca gtaatgtaaa aaaatggaga cttaagttga atgaacggga
      601 gtaaagcgaa aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag
      661 atgagacgcg gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt
      721 agatctcttc acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt
      781 tgccctttac ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa
      841 ctcgacgagt cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc
      901 tgtatcccgg cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca
      961 tctaagcagt catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc
     1021 aacctgactt tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca
     1081 ttgcctgacg cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg
     1141 ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac
     1201 ccttgccgcc cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg
     1261 tgacgtcctt agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca
     1321 cgataattgt cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg
     1381 gctcaacacc gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac
     1441 tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt
     1501 ctaactgacg cgtacgttcc agtactgagc gcactttatc agccagatta ttgctatcgc
     1561 ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggcgatag
     1621 caccttctcc ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga
     1681 tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg
     1741 tggagaaatc gcccatgctc agcacgcgta cgcgctcatc atacttctcg ccgaacagcg
     1801 ccatcgcacc tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat
     1861 tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg aatctcttct ggtttcatcg
     1921 cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg
     1981 atacatgagt acccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat
     2041 tcagacgaat acgggcgcga cgagcctcat caacatcagc ctgcacagcg tcgcccactt
     2101 tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat
     2161 cttccaccac gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc
     2221 cggattccgc atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga
     2281 tggcatcaac cgctttccca tcaacaaaca gcgcggtcac tttgccgttc agttccagat
     2341 ggtcatagcc tttaaattca gatgcactgt caacacggat cattgcgttg taatcggcac
     2401 caaagccgct ggcttcgcgc gcccgacgac gctgctcttc cattgctgct tcaaaaccag
     2461 cttcgtcaac tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc
     2521 cataggtgtc gtacagacgg aaagcagttt caccatccag cgtatcacca gaaagttttg
     2581 ccagctcttc atccagcaac gccagaccgc gctccagagt acgagcaaac tgctcttctt
     2641 cagtcttcag cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc
     2701 ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca
     2761 tattaccgtg acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat
     2821 tggacggcat tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta
     2881 cgcgcagcga tttattgctc agatcggttg cgccagtgac tttcgctacc gcctggatca
     2941 acgtgcggaa caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac
     3001 gctccagacc cataccggta tctacagacg gtttcggcag cggttccatc gtgccatcgg
     3061 cctggcggtt gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt
     3121 cttccggact tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg
     3181 tgcacgggcc gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg
     3241 cacctttgtt atcgccgatg cgaataatac gttcgcgcgg gatccctact tctttttccc
     3301 agatttcgta ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca
     3361 gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga
     3421 aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt
     3481 aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg
     3541 tagcgcggga ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc
     3601 cggcgttggt aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta
     3661 cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct
     3721 tgctcataat tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt
     3781 aaccgccggg ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa
     3841 aataccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga
     3901 tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa
     3961 ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat
     4021 tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctggcga
     4081 atacgcgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg
     4141 ctgtcatcga gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc
     4201 gtagcatcaa tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc
     4261 agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga
     4321 gcatatgccg ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaaag
     4381 caaaagggcc gcagatgcga cccaggtttt gctgataaac agatttaata gtcaatgagt
     4441 gtgcagaatg ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac
     4501 gccagtgctg gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc
     4561 cgtgcacaca ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg
     4621 ctacgccttc gctatcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca
     4681 actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag
     4741 ttgcattcgc tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt
     4801 tctcgatcag cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt
     4861 cgccgtagag gatctggaat tcagcctgtt taaacggcgc agcgattttg ttcttcacca
     4921 ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac
     4981 ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccagta gtggtttccg
     5041 ggttaccgaa catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt
     5101 tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa
     5161 ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca
     5221 cggagtcaac gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca
     5281 gtgcctgctc gccggtatcc ggctgggagc acagcaggtt gtcgatatcg acgcccagtt
     5341 tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt
     5401 cacgctgcgc tgcggcgatc acctgaagcg tcagcgtggt tttaccggaa gattccggtc
     5461 cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg
     5521 aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga
     5581 tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt
     5641 tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat
     5701 gttctgttga agcaattata ctgtatgctc atacagcatc aagtattttg tagaaattgt
     5761 tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcgccg
     5821 ctgaagcatt cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg
     5881 gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg
     5941 gcataatcag cacgagccgc tttcagtgca cctatcgcca tttccaccac gacgggttca
     6001 ctcaccgcgc catgctgcgc cagcgtctct tcacgtacgc cgatcatttg cgctttggct
     6061 tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta
     6121 atcactttcg ctacccaacc gccggtacaa gactcagcag ttgttacggt tgcgccacgg
     6181 gctttcagcg cctgcccaac ctgttcgctt aactgcatca gttcactgtc agtcatcaca
     6241 acctctgtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt
     6301 tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca
     6361 cgcccccctc ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg tcctaactgc
     6421 cagaccgcca ttgcgtaatg ggtgctgtgg ttataacggg taatggtata gaagttcggc
     6481 agaccgtacc agtactggta accggtaccg acatccagac gcagcaggct ggcttgttga
     6541 tggttgccca gcggctgctg tggcgttaaa cctgccgcgg caagctgcga aatgctgtac
     6601 ctggttttga agccattcgg taagcctggc gcctgaccgt ttgccatcac cgccacctga
     6661 tcgcctttca cccaaccgtg cgctttgaaa taattcgcca cgctaccgat ggcatcaacc
     6721 ggatcccata agttgatatg cccgtcgccg ctgaaatcca ctgcatattg tttgtaagaa
     6781 gaaggcataa actgaccgta gcccatcgca ccggcaaagg aacctttcag attgagtgga
     6841 tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca gctcaccaga gaaatactcc
     6901 gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatacg ggttttcccc
     6961 atcacacgcc cccagcgggt ttcgacgccg ataatcccga cgataatttc cggcggtacg
     7021 ccatacacct gccacgcgcg attcagcgcg tcttcatact gattccagaa aacaacgccg
     7081 ttctgcacgt tgtccggcgt aataaatttt ttgcgataac gcagccatgc gccgttcgga
     7141 cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa
     7201 tccagacgct tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg
     7261 ttcaccattc tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc
     7321 tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt
     7381 tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc
     7441 aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa
     7501 gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga tacggacttt
     7561 acataactca actcattctc ctcgctatcc tttcactcaa actttcaaat taaaatattt
     7621 atctttcatt ttgcgatcaa aataacacct ttaaatcttt caatctgatt agattaggtt
     7681 gccgtttggt aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc
     7741 atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg
     7801 tgaccggcat tcttccgctg ttgattagcc tgctggttat catgaacgca ctgattaatt
     7861 ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc
     7921 gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg accttaagcc
     7981 tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct
     8041 gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt gtttatcttg
     8101 gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagctatc
     8161 tgctggttgg tctggtcacc aacttcttcc gcggctgggt gaccgatctg accaccgcta
     8221 tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcatctggca ggagcaacat
     8281 catgacgcgt attcggatcg aaaaaggaac gggtggctgg ggcggcccgc ttgagctgga
     8341 agccacgccg ggcaaaaaaa tcgtctatat caccgccggt acccggcctg cgattgtcga
     8401 caaactggca cagcttactg gctggcaggc tattgacgga tttaaagaag gtgaacccgc
     8461 ggaggcagaa attggcattg cggtaatcga ctgtggcggc acattacgct gcggcatcta
     8521 tccgaaacga cgtattccca ccattaatat ccactcgacg ggcaagtccg gtccactggc
     8581 gcagtacatt gtggaagata tttatgtctc tggtgtaaaa gaagaaaaca tcactgtagt
     8641 gggtgatgcg acaccacaac cctcttccgt aggccgtgac tatgacacca gcaagaaaat
     8701 caccgaacaa agcgatggtt tactggcgaa ggtgggaatg ggtatgggtt ctgccgtagc
     8761 cgtgttgttt caatctggtc gtgacaccat cgacactgta ttaaaaacca ttctgccgtt
     8821 tatggcattc gtttcggcgc tcattggcat cattatggct tctggccttg gtgactggat
     8881 tgcccacggt cttgctccgc tggcgagcca tccactgggt ctggtcatgc tggcgctcat
     8941 ctgctccttc ccactgcttt cacctttcct cggcccaggc gcagttatcg cacaggttat
     9001 cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc tggctttacc
     9061 tgcgctgttt gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct
     9121 ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgttctgg tgagccgctt
     9181 tttaaccggc gcaccgactg tactgatcgc ctggtttgtc tccggtttta tctatcaata
     9241 gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtattggc gcgagtgcca
     9301 ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcct gcggatctcg
     9361 aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat cccggtttgc
     9421 aattttcact cgggcagcat cgctatccgg tgaccgccgt tggcagcgtg gcggaagaca
     9481 accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc
     9541 cgggcactgt ccatgtggca ggccctgtac ccgacgatat cgcgccggga tcgattttga
     9601 agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc
     9661 aaaccttagg cgcgttcctg tgccacggtc tggctgccga ggggtatcgc gtcgcggttg
     9721 tcgatattca gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc gaatatggtg
     9781 aaggtatggc gtacggtttt ggtgctgacg ccaccagtga gcaaagcgtt ctggcgctct
     9841 ctcgtggggt agatgaaatc tttggtcgcg tggatttgct ggtctacagc gccggaattg
     9901 caaaagcagc ctttatcagc gacttccagc tcggcgattt tgaccgttcg ctacaagtga
     9961 atctggtggg ttatttcctg tgtgcgcgtg aattttcgcg tttgatgatc cgcgacggga
    10021 ttcaggggcg cattattcag atcaactcga aatccggcaa agtgggcagc aaacacaact
    10081 ctggttatag cgccgcgaaa tttggtggcg tcggactgac tcaatcactg gcgctggatc
    10141 tggcggagta cggcattacg gtgcattcac tgatgctcgg taatctgctg aaatcgccga
    10201 tgtttcagtc gctattgcca caatatgcga ccaagctggg tatcaaaccg gatcaagtcg
    10261 agcagtatta catcgacaaa gtaccgctca aacgcggctg cgattatcag gatgtgctga
    10321 atatgctgct gttctacgcc agtcctaagg cgtcgtactg caccggacag tcgatcaatg
    10381 tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat ccgttaagga tctccgtgag
    10441 actaatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg ccactacatt
    10501 aaggaaaagt tatggtatcc gcactcatca ctgtcgccgt tatcgcctgg tgtgcgcaac
    10561 tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca ctatgccagc
    10621 aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc gtggtcgcca
    10681 tcgccctgga cgatcagcag cgcatcgccg acaccttgtt tatgaaagga ctgaccgtct
    10741 tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat ttacagcccg
    10801 atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg cttaaactga
    10861 aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt tgaaacctaa
    10921 atcaggtaat cacgcccatg aaacctcgtc agcgtcaagc cgccattctg gagtatctgc
    10981 aaaagcaggg aaaatgctcg gttgaagaat tggcgcaata ttttgacacc acaggcacaa
    11041 ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt acttatggcg
    11101 gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca ctcatcaaca
    11161 cccacaagaa agagctgata gcagaagcta ccgttggttt tattcatgat ggcgattcga
    11221 tcattcttga tgctggcagt accgttttgc agatggtgcc tctgctctcg cgctttaata
    11281 acatcacggt gatgaccaac agcctgcata tcgtcaatgc gttatctgaa ctggataatg
    11341 aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca tttcacgggc
    11401 agctggcaga gaatgccttc gagcatttca ccttcgataa attgtttatg ggcaccgacg
    11461 gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ttataccgtc agtaaggcaa
    11521 tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt ggccgtaaaa
    11581 gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac gcagatatcg
    11641 atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata accggagaga
    11701 gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga attgcaggaa
    11761 gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa tatcatcctg
    11821 cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat tggtaagaaa
    11881 atcgccgcaa cgcttgccag taccggcaca ccggcttttt ttgtccatcc ggcagaagcg
    11941 ctgcacggcg atctggggat gatcgaaagc cgcgatgtga tgctgtttat ctcttactcc
    12001 ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc tatcgcgctg
    12061 ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc ggtgctggat
    12121 atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag caccgtcaat
    12181 accctgatga tgggcgacgc actagcgatg gcagtcatgc aggcgcgcgg ttttaatgaa
    12241 gaagattttg cccgctccca cccagctggg gcactgggcg ctcgcttgct gaataaagtg
    12301 catcatctga tgcgtcgtga tgacgctatc ccacaggtag cgttaaccgc cagcgtgatg
    12361 gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg tgacgctcaa
    12421 caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt tggcggcggc
    12481 gcactcacca cgccagttaa tgaagcgatg acgacaggcg gtaccacatt acaggcacaa
    12541 agccgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc cgcaccggtg
    12601 gtagatgaaa acggcaaact cgtcggcgca attaacctgc aggatttcta tcaggccggg
    12661 attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc gacgtcggtt
    12721 tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc ctgacgaata
    12781 gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac gggcaccgcc
    12841 gccgcttctg gcggcggtaa cgtcacctca ggaaaagcaa aatgttgcgc ctcaagaatc
    12901 acttcatcgc cgttgcgggt ggctctcgcc agcactaccg cccgatgaat agcatgttcc
    12961 agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc tccggcactt
    13021 aataccacgc gggagagccc cagccgcaaa cgacactgct cgcagaaata ccccgccagc
    13081 agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa cacgctcagg
    13141 cggtggaaca agtcagcgcg aaatcgccct gccagcacct cttcgcgtaa atcgcggtta
    13201 gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac gcgctgaata
    13261 tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc
    13321 tcatcgagaa acagcgtgcc gttatccgcc atttcaaact tcccactgcg gttactgata
    13381 gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac actttccggc
    13441 agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc ctcatgaatc
    13501 gctttcgcca ccagctcctt acctgttccc gtctcaccgc tgatcaggac gttgagatcg
    13561 gacgccgcca caatctcaat ctcttttttc agttgcgtca tgcctggcga cagaccgatc
    13621 atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcaacat attctggctt
    13681 tccagttgct caatcagcaa cgcattgctt aacgctcccg ccgccagcgc ggcaatcagg
    13741 cgtaactctt cgtcactgaa aacatcgaac tgatcgggct gcatcccgtc gagcgtcaat
    13801 gcgccgatca ggttttgccc ggcaaacaat ggcagaccaa cgcaggcgtg gaccttcaga
    13861 ctctcctgtc cgggaatcaa accatcatag ggatcgggca attcgctgtc tgcgggaaag
    13921 cgcaccacat cacccgcgcg ggcaatcgct tccagccgtg gatgaccttc cagcgcaaag
    13981 cgtctaccga gtacatcctt cgccagcccg tcgatggcaa gcggaataaa ctgccgcgag
    14041 tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgcagcgt ggtgatcagg
    14101 cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat attcgccagc
    14161 acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat attgtcatat
    14221 cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt tatttataga
    14281 gtaaaaacaa tcagataaaa aactggcatg caatctgcta ttagcaagac atctttttag
    14341 aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac attcattggg
    14401 tgggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa acgctgcgcg
    14461 gcagcagcta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc gacaccgtcg
    14521 accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat ctggcggata
    14581 tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg accgaactga
    14641 tggcacaaat tcccgatacg ccgatctact gtacagccaa cgctatcgac tcgataaatg
    14701 gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac acgctggata
    14761 tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg ccggacagca
    14821 tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc ggtcagcact
    14881 actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc gagcagtgcc
    14941 agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg aaaattaccg
    15001 agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc gtggtctggc
    15061 gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat tatcaggaag
    15121 acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg gctgacgcta
    15181 tcgcccaggg gattgcggaa accgacccac gcgtggcggt gaaaattttc aacgtcgccc
    15241 gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg ctggtcggca
    15301 cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag gagatgaccg
    15361 gattacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg agcggcggtg
    15421 cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg cttagcctga
    15481 aagcgaaatg gcgaccagac caggacgctc tggagttatg ccgtgaacac ggtcgcgaaa
    15541 tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg gtagttaaag
    15601 aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc agcgtctgcc
    15661 agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca ggaacgccgt
    15721 ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa gacgtctttg
    15781 aagaactggc atcggaggca aaatgagtaa cggcattgtg atcattggtt cgggcttcgc
    15841 cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat taaccctgat
    15901 tgccgccgac agcatagatg agtacaacaa acctgacctc agccatgtta tcagtcaggg
    15961 gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgagc agtttaatct
    16021 gcacctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccgtg tggtgaaaag
    16081 ccagaataat cagtggcaat acgacaagct agtactggca accggtgcca gtgcctttgt
    16141 cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag agtatcgcgc
    16201 ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gttggcggtg gtttgattgg
    16261 tagcgaactg gcgatggatt tttgtcgtgc aggcaaaatg gttacgctaa tcgacaacgc
    16321 tgccagtatt ctggcgtcgt taatgccacc ggaagtaagc agtcgcttgc agcatcggtt
    16381 gacggagatg ggcgttcatc tgctgttgaa atctcagttg caggggctgg aaaaaacgga
    16441 ttctggcatt ctggcaacgc tggaccacca gcgcagcatc gaagtggatg cggtaattgc
    16501 cgccaccgga ctgcgcccgg aaacagccct ggcacgacgc gccgggctga cgattaatcg
    16561 cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg cgctgggcga
    16621 ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac ttagcgggat
    16681 ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactaccgg cgatgctggt
    16741 gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc gtcaggattt
    16801 acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg acgatgctga
    16861 ccagcttcgc gcctttgtgg taagtgagga tcggatgaaa gaggcatttg gattgttgaa
    16921 aacattgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg ctggcgcgtc
    16981 ttatccgacc tacggggacg catgtgtagg ccggataagg cgtttacgcc gcatccggca
    17041 agggtgtcca aatgcaacac gttttatccg ttctggactt cacccgctaa ccaacgcgcc
    17101 gtagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa actctgtgga
    17161 aagagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa acggttatga
    17221 ataaccccgc cgctaaatac cagcgtagtg ataccgtgca tcgtggcctg ctcacgcatc
    17281 aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg attaaccgga
    17341 gcctgccagt tcagccactg ctgccagaaa gtggcgagat ccagttgatt gtccaccagc
    17401 ggcatcgtca ccggatgcgt cactccgtcg catgaggctg cgagcgcctc cagagcacaa
    17461 gccgcttcac cttcataact taacgaagct ggcgcacagc ccagtgccgc cgccactgca
    17521 tcgaacaaac gcccacacga tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc
    17581 gccagcacgc tccagttttg ctgttgcaca ctcgctgttt cgggataatt ctgccactcc
    17641 ggcacaaagc gcaggcactg cgccagcagg tttcgccacg gctgcttcgc cgccaaatcg
    17701 ccaccagcaa gcgccaccgc aggcaagccg cccaggtgct ggcattcgcg atagttcacc
    17761 cgcaggcact cgccgcccca caaagcgccg ttctccccca taccgatacc gtcgagcgtc
    17821 aaagcaatga catcaccgcc ctccagtggc cagtgatgtt ctgccagaca cgccgccgcg
    17881 tgggcatgat gatgcagcac cgtttgcgtc ggcagattca tttcactcgc ccactggctg
    17941 gagacatagc ccggatgtgc gtcatgcaca acgtattgcg gggtgaaatc gtagatgttt
    18001 tgcatcaggc gtaacgcttc gcgccactgc atctggatgc catcgtcact taaatcgccc
    18061 agatgctggc tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt tttcagatcc
    18121 gcgccgagac acagcacagg cggaacattt ttaaagcccg gaggcaaagc cagcgcatcc
    18181 ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac cgaatcatcc
    18241 atccgctgca cgatgtcgcg gttatgtatc aagaatccgt cggcaatgcc ctgcaaatcc
    18301 gccagcgcct gttcgttgct gatagccggt ggtttaccgc tcaggttgcc ggaggtcatc
    18361 accagcgggc attgcagttc ctgtaacagc aaatgctgga gcgggttcgc aggcaacatc
    18421 accccgactt cgttaaggcc aggggcgata tcatcacaaa gctcaggtac gtatttttta
    18481 tccaccagca caatcggcgc ggcgggcgtg gtaagcaact ggcgcgcagc gtctggtaaa
    18541 ccgtcagcca ctggcaacat gaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc
    18601 agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc gccaatccct
    18661 ttgatggcga caatgttgcc catttttaac tgtgcgatag ctgcctgtaa tgccgcctct
    18721 tgttctgcat gttcaccatg acttacccat tcaagatgcg gaccacactc cgggcaggcc
    18781 accggctggg cgtggaagcg gcgatcgagc gggtcgcggt attctttatc acaggccgga
    18841 cacagcggaa acgccgccat tacggtaaac gggcggtcgt aaggcatggc gcgaataatg
    18901 gtgaaacgcg gaccacagtg agtacagttg ataaacggat aacgataacg ccgttcgcct
    18961 ggggtattca tttcggcaag gcaagcaggg caagtggcgg catccgggac aatttgcgta
    19021 ttcatggtgc cgcctgtgct ctggcggata gtgaactcag tgggcagttg tggccagata
    19081 aacggctcac gctcgacgct atcaatacgc gccagcggcg ggcagtgctg atgcaattga
    19141 acaagaaacg tttccgggtc ttccagcagc cggacttcta ctccatcgcc gtcattacag
    19201 acatcgccgt gaagatttaa ttgctgtgcc agttgccaga caaacggacg aaaaccgacg
    19261 ccctgcactt tgccacgaat acgcagttgg acaccgcaag atgtgttttt tgacattgag
    19321 ttattcccgc catcatgaat tgcgtaaccc gcccagccgg acacgacagc gtcgcatccg
    19381 gtagtcacag gtcggcgata ctgcccgctc cgtattctac gaatatttcc gggaattcct
    19441 ttgatgccag aacagttctg taagattttt agaacatcag cgccgtgcgg cgacgttttt
    19501 ctgcgctcag ttgttcaagt ttattacgat cgacacaaat cagcgcatga gtcgggcaag
    19561 ccgccataca cgccggaccg tcttcacgat ggttgcacag gtcgcattta ttggcttcgg
    19621 ctttgtcagc ccgtacattc agccctgcgc cgctgttgcg aatcaccgga cgtaccacca
    19681 cttccatcgc gccatacggg caagccacaa cgcaggtttt gcaaccaatg caacgttcct
    19741 gcatcacatg aacaaaccct ttatcacggc tgatagcacc attcgggcag acgttagcgc
    19801 acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca cctttaatga
    19861 catgaatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga ttttcctgat
    19921 gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc gcgtcagcaa
    19981 tgatgaaacg gttcatcaaa ttctccagca atgacagtta atgcgccgat acactcacaa
    20041 atcatgccag tttttaattc actgttattt aaggaaatta atttctgtaa tgcaggaaaa
    20101 aagatgtcat cgacactagt gacgatgaca tgtgatgaca atgtttatcg cgaaagagca
    20161 atgagtgagt cgcggcggat cagttttccg ctgaaagttt tcggcggtgt gtaagcgccc
    20221 ccatcgagca taaaaatcag ccgtccaata atttcctgaa tcatctctgt taccggaatt
    20281 tttacgctgg agagcgccgg aacggtgtag ggggcaattg cgatatcatc gaatccgata
    20341 actgacacct gctctggcac cgctacgccg cgctcgtgta acgctttcat cgcacctatc
    20401 gccatatcgt cgttactggc aactaacgcg ctaaatttag ccccacgttc gagcaacgtt
    20461 tctacccctt cggccccgct ggcgggcgtc catttaccgt tagcgataag tttttcattg
    20521 agcgcaatac catgctgcac cagcgcgtct ttatacccgg caagacgttc aatgctggtg
    20581 ggggaatcca tcgagccggt aaggaaagca atctcctgat gcccggcgtt tatcaactct
    20641 gccacggcgt taaagctggt ctgtttatga tcgcaccaga cgctatggct gctatttttg
    20701 cgcaggcggc gattaagcac cattatcggc tgactgtgcg cgtcaataat gtcatcgatc
    20761 tcatccacgc ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa atccagcaga
    20821 tactgaatcg cctggcgctc ttcttcggcg ctgtgtttac catctgccaa tagcaactgc
    20881 cgccctttct cttccgccat tcgcgcggca tgaaagagta attcactaaa ataaatgccg
    20941 tggtaaagcg tgttggtcac taccagcccc agcgtctgag tactcttcgc cgacagattg
    21001 cgcgccagca agtttgggcg gtaaccgctc tcttctaccg cctgaaacac gcgatcttta
    21061 gtctcctggc tgacgtagcc attacctgaa agcacgcggg aaacggtcgc ttttgaaacc
    21121 ccggcgcgct tcgccacttc cagcatcgtc gtcatcattt tcatcccttt acacgcaatc
    21181 aacgcagtgt actgcaccgt ttgccgattg tccttgcgca atcggtggga aaaaatattc
    21241 aggtgaccgg cttcacaaat ataaaaaatg aacaattaac tctcttgctt atttggtgac
    21301 aactattcat gattttgtga aaccggtttc ttaattccgt ttcagcatcg gcatttttcc
    21361 gtcacgtcga ctgataacaa ctacatctac cctactgata acaggataaa atccgatggc
    21421 caaaaattat gcggcgctgg cacgctcggt gatagcggca ctgggcggcg ttgataacat
    21481 ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt atcaaagatg atgcgcttat
    21541 cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc gtggtacgca gtgacaacca
    21601 gtgtcaggtg attatcggca ataccgtttc acaagccttt caggaaatcg tcagcctgct
    21661 gccgggagat atgcagcccg cacagcccgt aggtaaaccc aaactcacgc tacgtcgcat
    21721 tggtgcgggg atcctcgatg cgctgatcgg caccatgtca ccgctgatcc cggcgattat
    21781 cggcggatcg atggtcaaac tgctggcgat gatcctcgag atgagcggcg tgctgacaaa
    21841 aggatcgccg accttaacca ttctgaatgt gattggtgac ggtgctttct tcttcctgcc
    21901 gctaatggtc gcggcttcgg ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc
    21961 catcgcgggt gtgctggtac atccaagttt tattgaattg atggcgaaag cggcccaggg
    22021 tgaacatgtc gaatttgccc tgatcccggt caccgcggtg aaatacacct acacggtgat
    22081 cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac
    22141 accggcggtg accaaaaact tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct
    22201 ggcaatcctg ctgattggcc cgattggtat ctggatcggt agcgccattt cggcgctggt
    22261 ttacaccatt catggttatc tgggctggct ttcagtcgcc attatgggcg cgctgtggcc
    22321 tctgctggta atgaccggga tgcaccgcgt ctttacacca accatcattc agaccattgc
    22381 cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg
    22441 cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg gaactgcgcc agacggcgct
    22501 ggcagcagcg gcatcagcca ttatggcggg gatttccgaa ccggcgttat acggcgtggc
    22561 tcttcgcctg aaacgtccgc ttatcgccag tcttatcagc ggttttattt gcggcgcggt
    22621 tgccggtatg gcggggcttg ccagccactc aatggcagcg ccggggctat ttaccagcgt
    22681 gcagttcttc gatccggcga atccaatgag catcgtctgg gggttcgcgg tcatggcgct
    22741 ggcggtggtg ctgtcgttta tcctcacact attgctcggc tttgaggata ttcctgttga
    22801 ggaagcggct gccgaggcgc aaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt
    22861 aagtcttaat tgaggatgaa aatgtcagta tttccagaag gttttttatg gggcggcgcg
    22921 cttgccgcca accagtctga aggtgcgttc cgtgaaggtg gcaaaggact gaccacggtc
    22981 gatatgatcc cacacggcga gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt
    23041 cagttgcgcg atgacgagtt ttatcccagc catgaggcga cggattttta tcatcgttat
    23101 aaagaagata tcgccctgat ggcagagatg ggatttaagg ttttccgtac ctcaattgcc
    23161 tggagccgtc tctttccgca gggcgatgaa atcacgccca atccgcaagg cattgctttt
    23221 tatcgttctg tatttgaaga gtgtaaaaag tacggcatcg aaccgctggt cacgttgtgc
    23281 cacttcgatg tgccgatgca tctggtcacc gaatatggct cctggcgtaa tcgcaagctg
    23341 gtggagtttt tcagccgata cgccagaacc tgctttgaag catttgatgg tctggtgaaa
    23401 tactggttaa ccttcaatga aatcaacatt atgttgcata gcccgttttc cggcgcgggt
    23461 ctggtgtttg aagaaggtga aaatcaggat caggtgaaat atcaggccgc gcatcaccag
    23521 ctggttgcca gtgcgctagc caccaaaatc gcccatgagg ttaacccgca aaatcaggtg
    23581 ggctgtatgc tggcgggcgg taacttctac ccttacagct gcaaaccgga agatgtctgg
    23641 gcggcgctgg agaaagaccg ggaaaacctg ttttttatcg atgtgcaggc gcggggcgcg
    23701 tatccggctt actctgcccg cgtattccgc gaaaaagggg taaccatcga caaagcaccg
    23761 ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct ctttcagcta ttacgcctcg
    23821 cgctgcgcct cggcggagat gaacgccaat aacagcagtg cagcgaacgt ggtgaaatct
    23881 ctgcgtaacc cgtatctaca ggtaagcgac tggggctggg gtattgatcc actcggtctg
    23941 cgtatcacca tgaacatgat gtacgaccgt tatcagaagc cgctgtttct ggtggaaaac
    24001 ggcctcggcg caaaagatga acttgctgcc aatggcgaga ttaacgatga ctatcgcatc
    24061 agctatttac gcgaacatat ccgcgcaatg ggcgaagcga ttgcagacgg cattccgctg
    24121 atgggctaca ccacatgggg ctgtattgat ttagtttccg cctctacggg tgaaatgagc
    24181 aaacgctacg gttttgtcta cgtagaccgt gacgacgcag gcaacggcac gctgacgcgc
    24241 acgcgtaaga aatcgttctg gtggtataaa aaagtgattg ccagtaatgg tgaggattta
    24301 gagtaagcaa ccgtgccgga tgcggcgtga acgccttatc cggcctacat gtcgcacgaa
    24361 ctatgcaggc ccgataagca ctgcgcatcg ggcaatctgg agtttggcat cagtcaaaat
    24421 caaagaaacc attccggctt aacgccaaat cgggctgaaa gtgccttaat gtgggatatt
    24481 gtcagggatc gttgtccaga caaaatctga ctgaccaggg atttagagcc gatttcgttc
    24541 ttcaaatccg cataggtcag attatgttga tcaatcaacg tgcgaagtaa cgccacgcca
    24601 accggcattg ccgcaacggc tttgtcgaac tctgcaaact tttcgttgtt gttttcatac
    24661 tctgcaatgc ggctcgccag aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa
    24721 tattcgacca gttccagtgc ttcacgataa tcatcttctg agtcactgcc tcccagaaag
    24781 gggactgcat tgaccagttc tcttgttgct ttaacagcgc gtgcagcatt agcagtcatt
    24841 ctttgttctc tctgtagtaa cgtgtcagtt tgtcatactc tgcgtgattc gttatgtgct
    24901 tcacaaagaa ccgcttatta acaaagttta tgtaggcaat aaccctaaga ttgttcccgc
    24961 cgacatccag aacgtaccat ttgttgcgat atttaaaatt gtcgaggttg ggaaacgccg
    25021 ttcgcatttc ttctggcgta gaaaaatccg tttccttaat aacccgatac aatgcctgaa
    25081 gagccagagc gtcgttagga tatttacgcg cagactcttc aaatggcgct ttagaaatga
    25141 tgtgcatttt taagttttaa cttccttata gtaagtaaac agccacatct gcgagttcta
    25201 tcctcatgtg ttcggaaact atagctcatc aaatccaagt tcacaatatg taaacacttg
    25261 cgcgtgatat aattaccccc aaaatcctca taaccactct gcaaaaagtc agttctgatt
    25321 caccccatca agcacatccc cctttatcaa gcaacgttgc gtcagcgaca gatcttccgt
    25381 ttgtgtgtcg cgcccggcga tgggtttgag gacgagttta cggttggggg tataagtaaa
    25441 acgcgtgagc gcgggatagc ttaacttgcc aaaccagccg tgaacacgct gccatttggc
    25501 gtcttttacc tgatacagac tggttatcca gtaaccagaa ttaaactgca tatccagcaa
    25561 ttgcgtgtga ccattgcctt gtaaatccag caaatcatct acgccagttt tgcttgcagt
    25621 gtagaatccc cagggttcaa agacacaggg acggccttcg tgcgtgaacg tcatgaggat
    25681 taaatgtgca tagggcgcca gaccattacc ggcacttggc aaccagataa ccagatcctg
    25741 aatgccgtta cgatccagat cggcgcgata gagctgccct ttgcccgcca tatttgccag
    25801 caaccccact ggcaccgtcc aggatttacc cgcgcggtct ttagcggtta attgccagta
    25861 atcctcttca tttggcatct tctcaagcgt cacgccagca acgccgattt ttctaaaatg
    25921 ccaagtttgc tcggtataat aaggcatggt caggagaggt ttcagcgccc gcttttgcgc
    25981 atattcgctg ccactcattg gcagatattc ggtaaagctg gtggccagtg cagactgcac
    26041 taagctcaat aaaaaagcgc acatccctgt tctgatgttc aattccctga attccctaat
    26101 gaagaattat tcttccaccg ctaactgcgc aaatccgccg ttcccttccc agccttccag
    26161 tcgttgataa acggtttcta ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc
    26221 gacgatatcc ggctggatgc cgaggaaaat cacttcgcca atatcttctt tcaactgatc
    26281 gataaggtag ttcaacggca tgttatgggt agtcatcata aacatctcgg cgatatcatc
    26341 cgggtcgatg atgcggatct cgccggggtt tagccccata tcagtggcgt cgacaatcag
    26401 cagtcgtgtc gggcgcagtt cacggatagc gacaatgtcg ttttccggtg cgctaccgcc
    26461 gtcaatcacc acccagttac ctttcggcgc ggcggcgcac ttttccgcca gcagcggacc
    26521 tgcgccatca tcacccatca tgctattgcc aacacagagt aaaacgtcag tcacgcagtc
    26581 tcctcaccat caaatagatg gcgttttcct gatgaatatc atgcagcata cttaacagca
    26641 ttgtactcca ttcctgctgc tgcgaagttt gcgctgcccg ggctttatcg aatgcattgg
    26701 ccagcatcgg cacatggtta atgtcgatga cgatctcacc atatttcggc acaccttcca
    26761 ttttgcggcg ggcttcgctg cccgcctcca gagtggcaat ccatgccaga tattcatccc
    26821 acgggcaggt gagcgccgct tccaggcaat cgataacccc aaggtggtga ccaatcgcca
    26881 ggctgtaata gaccacctgc tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt
    26941 tacggctcag ttgactgaac accacctttt cactcatcgg atacgcgcct cttcaacaac
    27001 ttgattcaga tggctgacaa tctcgttcag acgcggatcg ttttccgcct ccagccagcg
    27061 tgcaacctgg tcttcgccct gccctaactg tgtcaggtaa tcatcggcaa tctggcgacc
    27121 gtaacgataa cccgccaggc ggcgtgcttc gcgatccact ttcacgcgca gcggctgcac
    27181 catatcgcca tgcagaatct ctgccggttg ttcatcctgc tcacccggcc cacgggcgtg
    27241 aattttctgc tccagcaggc cgagcgccat tgcaaagccg tacagcgtgg cggcaggcgt
    27301 tggcgggcag ccagggatat aaacatccac cgggacgatt ttatccgtac cgccccacac
    27361 gcagtagaga tcgtggaaga tcccgccgct gttaccgcag gcaccgtagg agatacagat
    27421 tttcgggtcc ggcgcggact gccacgcacg cagcgcaggg gatcgcattg cacgggtgac
    27481 cgcgccggta aacagtaaaa tatccgcatg acgcggtgaa ggaacgactt taatgccgaa
    27541 gcgttctgca tcaaacagcg gcgaaagcgt ggcgaaaatt tcgatttcgc agccgttgca
    27601 gccgccgcag tccacgcggt aaacgtaggc agagcgtttg atttttttca gtaacgacgc
    27661 cttcatactg gcgatggatt catccaccgt catcgggacc ggaatgccgt tggcgtcacg
    27721 ggggcctaat aaattgctca tcagatggcc tctttcatat ggcgagtcag ttcaatacgg
    27781 tcggacggca ccaggcattt ctggcgctta cattccgggc aagtctcaaa gctttcgcgg
    27841 tggttttccg cgcggctgtc gccgttgtgc ttaagcagcg caatggcgta gtcgatctct
    27901 ttctggacgg cgaaaggacg attgcagacg cgacagttgc acagcgcaaa acgggactgt
    27961 tgcaggaaat cttctttctt ccacaccgcc agttcgtact cttgcgacag tttgatcgcc
    28021 gccgtcgggc agacttcttc gcagcgtccg cagaagatgc agcgaccgag attaaactcc
    28081 caggccagct caccggtggc gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca
    28141 ttgacgcagg ccgcgcagcc gatgcactgc tgcgggttct gctctggctt accacggaag
    28201 tttttatcaa ccgcaatcgg ctccagcgga taagacgagg tcgccgtgcc ggttttgata
    28261 acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt tttacgctca
    28321 atgctgtaac gctcgagttc tttgtacggc accactttgc tcttcttctt acgcacatcg
    28381 accacggtca tgcggtcggt acaggagtag caagggtcga ggctaccgat aatcagcggc
    28441 gcatcggaaa cggtattgcc acgcagcatg tagcgcaggg tcggccagtt cgcgtaagtc
    28501 gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct ccagtggata
    28561 tcgtcgccgc gcggcgcttc ggcaaagccc agcgcaaagc ggtgcggaat gtaggtaaag
    28621 ccttccacca tcagcgggcc acccggcagg ttatccagac cgtagtcgat catgttcagc
    28681 gcggtataga cttcgttgat acgcactttc agacgggaaa taacgtcgca gccctgctcg
    28741 ctgtggactt ccattggcag caggccatag ccgacaaatg ggtgatcggc gcgggtatcg
    28801 cgggcgtgac cgctggcgcg gaccatcggg ccaacgttac tgaagtcgcg agcgatttcc
    28861 gggtccagac gaccaatgcc gacagtgcgc tgttccatgt tcggcgtact cagcagcaca
    28921 tccaccagct cctgcacttc acgacgcatc tgttgcgcca gttgacgggt ctggatcatg
    28981 tcatctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta ggttttacgc
    29041 gcgccggtaa ggatctccgc cattttcatg gaggtttcac gcacgcggaa gaactgcata
    29101 aagccggagt caaagccggt aaagtggcag gcaagaccga ggttgagcag atgcgagtgc
    29161 aggcgttcca cctccagcag aatggcgcgg atcatctgcg cacgttctgg cacctgaata
    29221 cccatcgcgt tttccaccga cgtggtgtag gcggtgctgt gagcaaagcc gcagatcccg
    29281 cacacacggt cagagaggaa ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt
    29341 tccatgccgc gatgaacgta gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg
    29401 acgaacagac ggaagtgacc cggttcgtca gaagtgacgt gcagcggacc aatcggcacg
    29461 acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc atcggtggtc
    29521 ggtgccggac gttgacgata atccatgctg tctttacgca gcggataaag ttcatccggc
    29581 cagtcatccg gcagcaccag acggcgttca tccggcagcc ctaccggaat caaaccgtac
    29641 atatcgcgca cttcgcgctc gccccacacc gccgccggaa cgcgcggcgt cacggacgga
    29701 tactccggtt tgttggcgtc aacttcgacg cgaaccgtta cccaacacct ggtgcccttc
    29761 tccatcgaca gcacgtaata aacggcgtaa tgaccattca gtttgcgttc gtcgttacca
    29821 aacagcaccg acagccagcc accctgtttg tagtaaagaa actccaccac ttccggcagg
    29881 tagttcacct ttacggtgac agtcagctga tctttggtct gccaggcgtg gtccagcacg
    29941 acgcccggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt ttcttcagac
    30001 atgctcaaac tctctttaat cacgccgcct gcaaggagac gaacgctaaa aatgcaaagc
    30061 caaacccagc ccaggtaatg cgtggagtaa tatcaagacg cagacgggcc atgctgtttt
    30121 cgaacagcgc gataaccagt acgccgacta ccagtttgac gatggcaatc accagcgcca
    30181 gtagcagtcc accgacggta aaggtttcca tttgtcccca cggaataaac accccgacga
    30241 acatctgcaa caccaccagc tgtttcaggc tgatacccca tttcatgacg ccaaagccgc
    30301 tgccgctata ttcagagagc ggaccttcct gcaactcctg ctcggcttcc gccagatcga
    30361 acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcacaaagc gccagcacca
    30421 gcgggatgct ctggctcagc ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg
    30481 tggaaccggc aacctgtgcg gcaacccaca gaccaagcag cagcatcggt tcgaccagca
    30541 cgccaagcat cgcttcacgg ctcgcgccga tagcagtaaa cgggctaccg gtatccagac
    30601 cggaaatggc aaagaagaaa cgcgcgatgg caaagagata cagtaaggtg atcaaatcgc
    30661 ccagttgcgg cagcggagaa ccaacggtca ccaccggcag cgcagtagcg atagtcagca
    30721 ttacgcccac catcacatac ggcgtcaggc ggaacaccca gccggaggca tccgggccaa
    30781 cgctctggcg gcccagcagt ttgataatgt cgcgatactc ctgcaacacg cccggcccgc
    30841 gacggttatg caagcgggcg cgcgccacgc gggttatacc ggagagcagc ggcgcaacgg
    30901 caaataacac cagcgcctga attaacggat ataaaacact cattctcagg ctcctcgtga
    30961 aacaataatc accaccagta ccgccagttc aaccagcgcc atccggcgga acagcaacgc
    31021 actcccctcg cactgccagc ccggcaccag cgacaccgga ttcagccatt tgcgcagttt
    31081 cagcaccggc gcaaacgcct gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat
    31141 tgatttttcg tggtcgtaac cgcacaccca ggccgcaccg cgggaacgcg atggcaaacg
    31201 atcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa tcagcagcaa
    31261 cgtgatcatc ggttgagaaa cggtggtgtt agcaggctcc agcggcagag gtacagcagc
    31321 agagagcatc ggcagtagcc acggcgcagc aacaccgcca attacgcagc aaatcgccag
    31381 tgccactacg cttacgctca tcaggagcgg tgcacaggtg gcgttttcgg cttctttggt
    31441 acgcggcgcg ccgaggaacg tgacgccata gactttcgcc atacacatca ccgccagcgc
    31501 accggtaatt gccagaccca cagcgagcag cggccccagc agacgggcaa caaacgcgcc
    31561 actattgctc agtttgaaaa aggattgata gataacccat tccccggcaa acccgttcag
    31621 cggcggcagt gcagccattg ccatcagccc gactaacata gcgatggaga taaccggcat
    31681 tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga accagacgct
    31741 ccccgccccg aggaacagta cacttttgaa caggctatgg ttaagcagat ggtacagacc
    31801 accgaccagg ccaagagcaa tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc
    31861 gcccagcccc agcaggataa tgccgatatt ttccagggtg tggtaagcca gtagacgctg
    31921 gatgttatgc tccatcagcg catacagacc accgacgaac gcggtgatca taccgagcac
    31981 cagcagcgcg atcccccacc acagcggtgc attaccgccc agcagtgaca gggttaaaat
    32041 gcccagcagg ccaattttca tgactaccgt agaaaacaac gcggcagctg gcgcagaggc
    32101 gttcgcatgt gcctgcggca cccagccgtg cagcggaata atcccggcca gcaggccaaa
    32161 gccaatcaca ccgagcagcc agatatcgga accgagcggc agctgttgca tacgcatatc
    32221 cagcaggcgc agatccagcg tgccgtaacg ctgccacagc agccagcaag caatcgccag
    32281 cagcagagtg ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc tgttgctggt
    32341 gaggaacacc gcacacaagg ccatgacttc ggccattacc acgaacatgc cgaggttgct
    32401 ggcaatgacg gcgcagacgg cggcagccat caacatattg atctgcaagc cgttgcactt
    32461 cacctgcgcg tggcgatgcc agtcaatgtt gtagaggctg acaaacagac cgcacagacc
    32521 gagcgtaatc agccaaatcg cgttaagcgg agagatttgc acatcgtagc ttaccagcga
    32581 cagcgcaccg ctcacgccaa ccgcgccagt cagtactgtg aagcccgctg ctgccgtata
    32641 cagactacca accgcgccgc caattccggc tatccagccg cttagcgcct tttgaaaaga
    32701 aaagagaaat gccagaacag cggcggcgac aaaccacgcc acgccgctat tgatcaggga
    32761 aattgcgctc atttagcctc tccactttga gcctgctgaa acaaggtgag atcgccaaag
    32821 tccgtgttaa aggtcagctc acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc
    32881 accagatgca gggctttagt cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag
    32941 ctacaaaggt cacatttgac ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg
    33001 ctgacacgcg ccggagcagg cggtgccggt ggcgctttcg gggtattggc gtttgccgga
    33061 atatccagcg gacggctgcc ggaaaattca attgcgccaa acgggcaggc gatgccacac
    33121 agtttgcagc ttacgcacag gctttcattc aactgcacgg ccccatcaac gcgggtgatg
    33181 gcgttaaccg ggcagaccac cgcgcagggt gcatcttcac agtggtgaca gagctgcggc
    33241 gcagattctt tttcatttag catcactctc aggcgcggca ttgattgcag gccgtgctgg
    33301 cgatgcgtct ctgaacaggc ggcctcacaa gtgtggcagc cgatacagag cgtggagtca
    33361 gcaattacaa aacgattcac caggcattcc tcaggtgatt gtcatttttg acgaaaacat
    33421 gccgatgaaa tgtcattttc gacactcatc gacacgccca tccccaaaca ggcgtaacgc
    33481 ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt tgtcccttta ccagttggct
    33541 taaattcacc ggcacattgt tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat
    33601 tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg atgttgacct tgccatcaat
    33661 catgccgtca atgctcagtt gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt
    33721 ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc gccagggtga cgtaaatacg
    33781 aaaatgttca aaaaggacga aacagagttc tttgtccggc gcgcagctca tggcgtgatg
    33841 caggcgcgct ttcgataacg tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg
    33901 gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta tttcccaaat
    33961 agtcattgtc aggttacccg tttaacagag atgccagctt taagcatttt ttgtgccaac
    34021 ttttaattca ttgttattaa agcgattttt aagattttgg tgaatttcgt cgccgtgtcg
    34081 acgtgtcatt tcgacatcat cgacattatt cactgcaggg ataatcaaca ctggcacaat
    34141 tattgcttgt agctggcaat agttaatggg aggcgatatg cacgaaataa ccctctgcca
    34201 acgggcactg gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg
    34261 ggtctggctc aaaattggcg cattttcttg tgtcgaaacc agctctcttg ccttttgttt
    34321 tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga
    34381 ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc agcgcgtccg
    34441 ccgctgtcca cagtgtcatg gtgacatgct acagattgtg gcagacgacg gtttacagat
    34501 tcggcggata gaaatagacc aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg
    34561 caacctgtat atcgagggtg atgaacataa ccctcattcc gcgtttcgta gcgcgccatt
    34621 tgccccggcg gcacgcccga agatgaaaat caccggcatt aaagcgcctg aatttacccc
    34681 cagccagact gaagaaggcg acctgcatta cggtcatggc gaagcgggca ctcacgcacc
    34741 gggcatgagc cagcgtcgga tgctggaagt cgaaattgac gtgctggaca aaaataaccg
    34801 tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag caactggtgc tcaacctggt
    34861 ttccagccca ggttccggta aaaccaccct gctgacggaa accttaatgc gcctgaaaga
    34921 cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat
    34981 tcgcgctacc ggcacaccag cgattcaggt gaacaccggt aaaggctgcc atcttgacgc
    35041 acagatgatt gccgacgccg caccgcgtct gccactggac gataacggta ttctgtttat
    35101 cgaaaacgtt ggcaacctgg tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt
    35161 ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg aaatatccgc atatgtttgc
    35221 cgccgcctcg ctaatgctgc tcaacaaagt tgacctgctg ccgtatctta actttgacgt
    35281 tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa attgaaatca tccttatttc
    35341 cgccaccagc ggcgaaggga tggaccagtg gctgaactgg ctggagacac agcgatgtgc
    35401 ataggcgttc ccggccagat ccgcaccatt gacggcaacc aggcgaaagt cgacgtctgc
    35461 ggcattcagc gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg
    35521 cgcgtgggcc agtgggtact ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc
    35581 gaagcacgcg acactctcga cgccttacaa aacatgtttg atgttgagcc ggatgtcggc
    35641 gcgctgttgt atggcgagga aaaataatgc gttttgttga tgaatatcgc gcgccggaac
    35701 aggtgatgca gttaattgag catctgcgcg aacgtgcttc acatctctct tacaccgccg
    35761 aacgccctct gcggattatg gaagtgtgcg gtggtcatac ccacgccatt tttaaattcg
    35821 gcctcgacca gttactgccg gaaaacgttg agtttatcca cggtccgggt tgcccggtgt
    35881 gcgtactgcc gatgggtaga atcgacacct gcgtggagat tgccagccat ccggaagtca
    35941 tcttctgtac ctttggcgac gccatgcgcg tgccggggaa acagggatcg ctgttgcagg
    36001 caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc gatggatgcg ttgaaactgg
    36061 cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt aggttttgaa accaccatgc
    36121 cgaccaccgc tatcactctg caacaggcga aagcgcgtga tgtgcagaat ttttacttct
    36181 tctgccagca cattacgctt atcccgacgc tgcgcagttt gctggaacag ccggataacg
    36241 gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt tatcggcact gatgcctata
    36301 attttatcgc cagcgatttt catcgtccgc tggtggtcgc tggtttcgaa ccccttgatc
    36361 tactgcaagg cgtggtcatg ctggtggagc agaaaatagc ggcccatagc aaggtagaga
    36421 atcagtatcg tcgggtggtg ccggatgccg gtaacctgct ggcgcaacag gcgattgccg
    36481 atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg
    36541 gcgtgcacct gacgccggat tatcaacgat tcgatgccga agcacatttc cgcccggcac
    36601 cgcagcaggt ctgcgatgac ccgcgtgcgc gttgtggtga agtattaacg ggcaaatgta
    36661 agccgcatca atgcccgctg tttggtaaca cctgtaatcc tcaaaccgcg tttggtgcgc
    36721 tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg
    36781 aagcgtgaat aatatccaac ttgcccacgg cagcggcggc caggcgatgc agcaattaat
    36841 caacagcctg tttatggaag cctttgccaa cccgtggctg gcagaacagg aagatcaggc
    36901 ccgtcttgat ctggcgcagc tggtagcgga aggcgaccgt ctggcgttct ccaccgacag
    36961 ttacgtcatt gacccgctgt tctttcctgg cggtaatatc ggcaagctgg cgatttgcgg
    37021 cactgcgaat gacgttgcgg tcagtggcgc tattccgcgc tatctctcct gtggctttat
    37081 cctcgaagaa ggattgccga tggagacact gaaagccgta gtgaccagca tggcagaaac
    37141 cgcccgcacg gcaggcattg ccatcgttac tggcgatact aaagtggtgc agcgcggcgc
    37201 ggcagataaa ctgtttatca acaccgcggg catgggcgca attccggcga atattcactg
    37261 gggcgcacag acgctaaccg caggcgatgt attgctggtt agcggtacac tcggcgacca
    37321 cggggcgact atccttaacc tgcgtgagca gctggggctg gatggcgaac tggtcagcga
    37381 ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac attcccggcg tgaaagcgct
    37441 gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat gagttcgcgg cagcctgcgg
    37501 ttgcggtatt gaaatttctg aatcagcgct gccggttaaa cctgccgtgc gcggcgtttg
    37561 cgaactgctg ggactggacg ccctgaactt tgccaacgaa ggcaaactgg tgatcgccgt
    37621 tgaacgcaac gcggcagagc aagttttggc agcgttacat tcccatccac tgggaaaaga
    37681 cgcggcgctg attggtgaag tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg
    37741 cgtgaaacga accctcgatt taccacacgc cgaaccgctt ccgcgtatat gctaataaaa
    37801 ttctaaatct cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga
    37861 agaacaaaat gtcatataca ccgatgagtg atctcggaca gcaagggttg ttcgacatca
    37921 ctcggacact attgcagcag cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg
    37981 taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac
    38041 gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat taaatatgaa gacgaaactg
    38101 ttctggcaca cggtccggta cgcagcattt tgtcgcgccc tgatacgctg cattgcagtt
    38161 acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg tgggctatac ccaaaatttg
    38221 gtcactattg cctgatgcca ctggcggcgg aagggcatat ttttggtggc tgtgaattta
    38281 ttcgttatga cgatcgcccc tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc
    38341 agatcgtttc tgtcgtcacc gaacaaatcc agagtcgcgt cgttaacaat gtcgactatg
    38401 agttgttatg ccgggaacgc gataacttcc gcatcctggt cgccatcacc aacgcggtgc
    38461 tttcccgcct ggatatggac gaactggtca gcgaagtcgc caaagaaatc cattactatt
    38521 tcgatattga cgatatcagt atcgtcttac gcagccaccg taaaaacaaa ctcaacatct
    38581 actccactca ctatcttgat aaacagcatc ccgcccacga acagagcgaa gtcgatgaag
    38641 ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat gctgctgatt aatctccacg
    38701 agcgggatga tttagccccc tatgaacgca tgttgttcga tacctggggc aaccagattc
    38761 aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg
    38821 cgcaatgtga agagaaagtg tttaccacta ccaatctgaa tttactgcgc cagattgccg
    38881 aacgtgtggc aatcgctgtc gataacgccc tcgcctatca ggaaatccat cgtctgaaag
    38941 aacggctggt tgatgaaaac ctcgccctga ccgagcagct caacaatgtt gatagtgaat
    39001 ttggcgagat tattggccgc agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg
    39061 tagcgcaaag tgacagtacc gtgctgatcc tcggtgaaac tggcacgggt aaagagctga
    39121 ttgcccgtgc tatccataat ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact
    39181 gcgcggcgat gcctgccgga ttgctggaga gcgatctgtt tggtcatgag cgtggagctt
    39241 ttaccggtgc cagcgcccag cgtatcggtc gttttgaact ggcggataaa agctccctgt
    39301 tcctcgacga agtgggcgat atgccactgg agttacagcc gaagttgctg cgtgtattgc
    39361 aggaacagga gtttgaacgc ctcggcagca acaaaatcat tcagacggac gtgcgtctaa
    39421 tcgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga ccgtgagttc cgtagcgatc
    39481 tctattaccg cctgaacgta ttcccgattc accttccgcc actacgcgag cgtccggaag
    39541 atattccgct gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata
    39601 tcgacagcat tcctgccgag acgttgcgca ccttgagcaa tatggagtgg ccgggtaacg
    39661 tacgcgaact ggaaaacgtc attgagcgcg cggtattgct aacacgcggc aacgtgctgc
    39721 agttgtcatt gccagatatt gctttaccgg agcctgaaac gccgcctgcc gcaacggttg
    39781 tcgctcagga gggcgaagat gaatatcagt tgattgttcg cgtgctgaaa gaaactaacg
    39841 gtgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc acgactctgc
    39901 tgtcacggat gaagcgactg ggaattgata aatcggcatt gatttaactg caaattgccg
    39961 gacagatcta cctgtccggc atactattca cgaggttttt tcggacgata tttttccggc
    40021 agttctggca ccggacgctt gtcatcgatg agatgacgca cggttaagat cggatgacgc
    40081 cacagcattc tcggcccggc ccaacgcata atctgtttca tctcttcacg ttttgcaggc
    40141 tggtaacagt gcaccggaca ctgcttacag gctggtttct gttcgccgaa cacacattta
    40201 tccagccgct tttgcgcgta agcaaacaac gcctcgtaat gctccggctc cgctgacgct
    40261 tgcgggcatt tcgcttgata aagatcgatc atttttttaa tcgtcagttt ttcacgagag
    40321 atacgcttgc cggacatgct gcctccacct cattaagatg catttatatt acatcttaat
    40381 cttaaagggc actatgactc taaagaagaa gggttcgcca accgatacaa ttttgcgtac
    40441 ttgcttcata agcatcacgc aaaagctgca aaacagcatc tttcccggaa ccagcatcaa
    40501 gaactcgccg ttcgcttctt cccctgaaat gattaactcc ggtatcatgt gcgccttatg
    40561 tgattacaac gaaaataaaa accatcacac cccatttaat atcagggaac cggacataac
    40621 cccatgagtg caatagaaaa tttcgacgcc catacgccca tgatgcagca gtatctcaag
    40681 ctgaaagccc agcatcccga gatcctgctg ttttaccgga tgggtgattt ttatgaactg
    40741 ttttatgacg acgcaaaacg cgcatcgcaa ctgctggata tttcactgac caaacgcggt
    40801 gcttcggcgg gagagccgat cccgatggcg gggattccct accatgcggt ggaaaactac
    40861 ctcgccaaac tggtgaatca gggcgagtcc gttgccatct gcgaacaaat tggcgatccg
    40921 gcgaccagca aaggtccggt tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc
    40981 agcgatgaag ccctgttgca ggagcgtcag gacaacctgc tggcggctat ctggcaggac
    41041 agcaaaggtt tcggctacgc gacgctggat atcagttccg ggcgttttcg ccttagcgaa
    41101 ccggccgacc gcgaaacgat ggcggcagaa ttgcaacgca ctaatccggc ggaactgctg
    41161 tatgcagaag atttcgccga gatgtcgctg attgaaggcc gtcgcggcct gcgccgtcgc
    41221 ccgctgtggg agtttgaaat cgacaccgcg cgccagcagt tgaatctaca atttggcacc
    41281 cgcgatctgg tcggttttgg cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt
    41341 ctgttgcagt atgcgaaaga tacccaacgt acgactctgc cgcatattcg ttccatcacc
    41401 atggaacgtg agcaggacag catcattatg gatgccgcga cgcgtcgtaa tctggaaatc
    41461 acccagaacc tggcgggtgg tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc
    41521 acgccgatgg gcagccgtat gctgaaacgc tggctgcata tgccagtgcg cgatacccgc
    41581 gtgttgcttg agcgccagca aactattggc gcattgcagg atttcaccgc cgggctacag
    41641 ccggtactgc gtcaggtcgg cgacctggaa cgtattctgg cacgtctggc tttacgaact
    41701 gctcgcccac gcgatctggc ccgtatgcgc cacgctttcc agcaactgcc ggagctgcgt
    41761 gcgcagttag aaactatcga tagtgcaccg gtacaggcgc tacgtgagaa gatgggcgag
    41821 tttgccgagc tgcgcgatct gctggagcga gcaatcatcg acacaccgcc ggtgctggta
    41881 cgcgacggtg gtgttatcgc atcgggctat aacgaagagc tggatgagtg gcgcgcgctg
    41941 gctgacggcg cgaccgatta tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc
    42001 ctggacacgc tgaaagttgg ctttaatgcg gtgcacggct actacattca aatcagccgt
    42061 gggcaaagcc atctggcacc catcaactac atgcgtcgcc agacgctgaa aaacgccgag
    42121 cgctacatca ttccagagct aaaagagtac gaagataaag ttctcacctc aaaaggcaaa
    42181 gcactggcac tggaaaaaca gctttatgaa gagttgttcg acctgctgtt gccgcatctg
    42241 gaagcgttgc aacagagcgc gagcgcgctg gcggaactcg acgtgctggt gaacctggcg
    42301 gaacgggcct ataccctgaa ctacacctgc ccgaccttta ttgataaacc gggcattcgc
    42361 attaccgaag gccgccatcc ggtggttgaa caggtgctga acgagccatt tatcgccaac
    42421 ccgctgaatc tgtcaccgca gcgccggatg ttgattatta ccggcccgaa catgggcggt
    42481 aaaagtacct atatgcgcca gaccgcactg attgcgctga tggcctacat cggcagctat
    42541 gtaccggcac aaaaagtcga gattggacct atcgatcgca tctttacccg cgtaggcgcg
    42601 gcagatgacc tggcttccgg gcgctcaacc tttatggtgg agatgactga aaccgccaat
    42661 attttacata acgccaccga atacagtctg gtgttaatgg atgagatcgg gcgtggaacg
    42721 tccacttacg atggtctgtc actggcatgg gcgtgcgcgg aaaatctggc gaataagatt
    42781 aaggcattga cgctgtttgc tacccactat ttcgagctga cccagttacc ggagaaaatg
    42841 gaaggcgtcg ccaacgtgca tctcgatgca ctggagcacg gcgacaccat tgcctttatg
    42901 catagcgtgc aggatggcgc ggcgagcaaa agctacggcc tggcggttgc agctctggcc
    42961 ggcgtgccaa aagaggttat taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt
    43021 tcgccgaacg ccgccgctac gcaagtggat ggtacgcaaa tgtctttgct gtcagtgcca
    43081 gaagaaacct cacctgcggt cgaggcactg gaaaacctcg atccggattc actcaccccg
    43141 cgtcaggcgc tggagtggat ttatcgcttg aagagtctgg tgtaattata attcccggta
    43201 gaaaaatact atcgggaata ttatctacag ctgacgaatc aaataaaaat accctgtata
    43261 ataggaaagc ttattttaca gggtaaaacc atgccatcta cacgctatca aaaaatcaat
    43321 gcccatcact atcgccatat atgggtcgtt ggtgatattc atggtgaata tcagttatta
    43381 caatcccgct tatatcaact ctctttttgc cctgaaaccg acttacttat ttctgtcggc
    43441 gataatattg atcgtgggcc ggagagtctt aacgtcctgc gcctgctaaa ccagccctgg
    43501 tttatctccg ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc gaccggcgat
    43561 ggcaatatgt ggcttgccag cggtggtgac tggtttttcg atttaaatga ttcagagcaa
    43621 aaagaagcta cagatctgtt gctgaaattc catcaccttc cacatattat tgaaatcact
    43681 aacgacaaca taaaatatgt catcgcacat gcagattatc cggggagtga atatctcttt
    43741 ggtaaaggaa tagcggagat cgaattactc tggcctgttg atcgtgtgca gaaatcgctt
    43801 aatggcgagt tacaacaaat aaacggcgct gattatttta tatttggaca tatgatgttt
    43861 gataacattc agacgttcgc taaccagatt tatattgata ccggatcgcc gaacagcagg
    43921 cggctgtcat tttataaaat aaagtaatga ggccgggtaa agcaaagcca ctacccggca
    43981 ctttttatta gcgcttacct tccgccagca gcggcggaat gctcggcacc attggcgcgt
    44041 catcaatatc tttctgcgtc atgcggaacg cttccgggta atgttcgcgg ctgatgcgac
    44101 gtagcggttc ggtatcgcgc caggtataaa ggcaatgctg gcactgatat accgtccaga
    44161 catctttcac cggcgatttc gccatcactt caatctgttc atcggcacaa cgtggacaaa
    44221 tcatcttgtt ctccttattt acgtgcggcc agcatagcgg tcagtttttc agcccaggct
    44281 ttggtttccg gcaaatccac caccggctgg ctgtagtgac cacggttgtc cggggcgaca
    44341 ggcgtggtgg cgtcgataat cagcttgtcg gtgatccccg ccgggcttga gccagggtcg
    44401 agttccagca cggacatatt cggcaactgc accaaatccc ctgccggatt tactttcgag
    44461 gagagcgccc acatcacctg cggcaggttg aacgggtcaa cgtcttcatc gaccataatc
    44521 accatcttta cgtagcccag accgtgcggc gtggtcatcg cacgcaggcc caccgcgcgg
    44581 gcaaagccgc cgtagcgttt tttggtggag ataatcgcca gcaggccgtg ggtgtacatg
    44641 gcgtttaccg cctgcacttc cgggaactcg gctttcagtt gttgatacag cggtacgcag
    44701 gtggctggcc ccatcaggta gtcgatttcg gtccacggca tgccgaggta cagcgattcg
    44761 aaaatcggcc tggtgcggta agagacttta tcgatgcgca ccacggtcat gttacgcccg
    44821 ccggagtagt gtccggtaaa ctcaccgaac ggcccttcga tttcgcgttt acggctttcg
    44881 atgacccctt cgaggatcac ttctgaaccc cacggcacat caaaaccggt cagcggggcg
    44941 gtggcgatcg ggtacgggct ttcacgcagc gcgcctgcca tttcatactc agactgatcg
    45001 tatttcagcg gcgtggcccc catcagcgtg atgatcggat cgttaccgag cgtgatcgca
    45061 atcggcagat cttcaccgcg ctcttctgct ttatgcagat gcagggcgat atcgtgcatc
    45121 ggtaccggtt gcaggccaag cttacgcttg cccttcactt ccatgcggta gatgccgacg
    45181 ttctgcttgc cgaagttatc tgggtcgagc ggatcgcggg aaaccacgca ggctttgtcg
    45241 agatagaaac cgccatcgcc atcatttaaa cgaaacagcg gcaggatatc gaacagatta
    45301 atctcgtcac catcgacggt gttctgcgcc caggcaggat tggcgcggcg ctccggggca
    45361 atcgggaagt tatcccagcg gcggataaac tcatcaatct gttttttaac cggcgtgttt
    45421 ggcggcaggc cgagggaaat cgcgtggttc tgctaggaac cgatggtatt catcgccacg
    45481 cgggcatcgg taaagccgcg aatattatca aaccacagcg cgggcgcgcc gtcgccgata
    45541 cgcccggtgg cgttagctgc tgctgccaga tccggctcag cgttcatttc ttcgctgatt
    45601 ttcagtaact ggccgtggtc atcaagcgcc tgtaaaaagc tgcgtaaatc atcaaatgcc
    45661 attattcatt ctcctgagaa aaattccgga cctgcggcaa tccttgccag cgcctggcgt
    45721 ggggatgttc gaagccaaat tgatccagca cgcgggctac cacatggtgg acaatgtcat
    45781 ctaccgtttc gggatggtta taaaaggcag gcatcggcgg caccatcgcc acgcccatgc
    45841 gtgaaagtgc gagcatattt tcgagatgga tggtgctaag cggcatttca cgcggcacca
    45901 gcaccagttt gcggccttct ttgagcacga cgtccgccgc gcgccctacc aggccatcgg
    45961 cgtaaccagc gcggataccg gcgagcgttt tcatactgca cggaataacg atcatgccgt
    46021 cggtacgaaa ggaacctgag gagatggtcg ccgcctgatc cgccgggtta tggctgaagt
    46081 cagcgagggc agcaacatcg cgggcgctgt aaggcgtttc cagttcaatg gtggttttcg
    46141 cccacttcga catcaccaga tgagtctcga cattcggcat ctcccgcagc gcttgcagta
    46201 atgccacacc aagaggcgca ccggtagccc ctgtcatccc gacgatcagt ttcattgctt
    46261 actctcttaa ttgttcgtat acgaacatta ttattaaaat acgcctctgt tctcattgcg
    46321 gcaagcactg aaaagtaaca acatcccctt cgtcactgaa aaaccgctat gatagcggca
    46381 gatagttgcc cggagtttac atggcgttac gaaataaagc gttccatcag ttacgacagc
    46441 tttttcagca gcacacggct cgctggcagc acgagttacc tgacctgacc aaaccacagt
    46501 atgcggtgat gcgcgccatt gctgataagc ctggtatcga acaggtggca ctgatagaag
    46561 cagcagtcag caccaaagca acgttggcag aaatgttggc aagaatggag aatcgtggct
    46621 tagtcagacg tgaacatgat gccgctgaca agcggcgtcg ctttgtctgg ctgactgccg
    46681 aaggagaaaa gatacttgct gcggcgatac cgattggtga cagcgtggat gaggaatttt
    46741 tggggcgttt gagtgcagaa gagcaggagc tgtttgtgca gctggtgcgc aagatgatga
    46801 acacatagga tgcaaattgc cgggtaggac gctgacgtgt cttatccagg cgacaaaaac
    46861 acaagcttcc caaacaaaag aaaaaggcca gcctcgcttg agactggcct ttctgacaga
    46921 tgcttactta ttcgcggaac agcgcttcga tattcagccc ctgcgtttgc aggatttcgc
    46981 gcaaacggcg caggccttca acctgaatct ggcgaacacg ttcacgggtg aggccaattt
    47041 cacgacctac atcttccagt gttgccgctt cgtaccccag caaaccgaat cgacgtgcca
    47101 gcacttcacg ctgtttggcg ttcagctcga acagccattt gacgatgctc tgcttcatat
    47161 cgtcatcttg cgtggtatct tccggaccgt tctctttttc atcggccagg atgtccagca
    47221 acgctttttc ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac
    47281 gaagcatacg gctgacgtca tcaactggct tatccagttg ctctgcgatc tcttccgcac
    47341 ttggttcatg gtccagctta tgggacaact cacgtgcggt tcgcaggtaa acgttcagct
    47401 cctttacgat gtgaatcggc aaacgaatag tacgggtttg gttcataatc gcccgttcaa
    47461 tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa
    47521 acttctctac cgcgcggatc agccccaggt tgccctcttc gataaggtcc agcaacgcca
    47581 gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta ctctcgatca
    47641 tccggcggcg agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa acttcttctt
    47701 cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc gcgtccaaca
    47761 cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta tcactgggtt
    47821 cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca aattccgcat
    47881 cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc ctacccgtga
    47941 tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct gcggcaaata
    48001 acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc gtgttgaact
    48061 ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt gttgttcccg
    48121 gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat gtttgatgat
    48181 aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacgcggc catctgcggt
    48241 cgcgataatt gcctgtcctt tgctgcctgc aatatcaatc cccttgttgc ccccctcaga
    48301 agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg agataggcgt
    48361 actggttgat gtactgctga cagtcggctc ggttgtgctt gctgttggta ccgttacagg
    48421 cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac attttgttag cactctgttc
    48481 acccgaagac tcagaatacg taattgtcgg ttgcgacgca acagcaacgg tggaattttg
    48541 tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc
    48601 agtgattggc gtaccggaag cattacccac ctgcaaggtc tgaccaacgt tcagcgcgta
    48661 tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag taatccaggc
    48721 gatatagaaa agcgtgtcgc cttttttcac ggtataggta ctgccgctat aactgccttt
    48781 cggaatgttc ccatactgac ggttatagac gatgcgtccg ttttccatct gtaccggctg
    48841 ctgagctact ggctgcactg gctggatttg cggttgttga gtggcctgaa tttgtggctg
    48901 ctgtaccggc tgaatttgcg gttgctgggc tgtagacgtc gtccccattt tcggcggcgg
    48961 cgtaatcaac ataccagaat tggtatttgc aggcgcattg ccattaacgg agctgaccgg
    49021 tgccggtgga tttgaagtgt cagaacagcc tgccagccat agcgaaacca gtgacaaagc
    49081 cgcaatgcgg cgaacggtga attttgggct tcccgcgctc atttatcccc caggaaaaat
    49141 tggttaataa ccagtgacat aattaccgtg caaggcacca tactgaacac tggaaaagat
    49201 gttcacgata cgctgacctg cggcaaaata accaggaaaa atccaggtat ttcctcacgt
    49261 tttaagccag ctcacccttc actaaaggga caaagcgcac ggcctccacg gtatcgataa
    49321 taaattcgcc tccccgacga cgcacccgtt tcaaatactg gtgctcctcc cctacgggta
    49381 agacgagaat cccgccttcg tccagctgcg tcattagcgc agttggaatt tccggcggtg
    49441 ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg ccaaccttgc catccatcgc
    49501 catgacgggt tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc gcctgccact
    49561 gcaaaccttt aatccgttcg accgagcaaa catgctggac aagatgcgcc aggattgccg
    49621 tttgatatcc cgaaccagtg ccaatttcca gcacccgcga ctgcggcgtc agctcgagta
    49681 attcggtcat tcgcgccacc atatacggct gcgaaattgt ctgcccctga cctatcggca
    49741 aagcgatatt gtcccaggct ttttgttcaa acgcttcatc aacgaatttt tcacgcggca
    49801 cggcagcaag tgcattcagc acctgctcat cctgaatacc ttgcgcacgt aattgatcca
    49861 gaagtgcttg tacgcgtctg cttaccattg cgtgccaact cccacgctgt ttaaccagtc
    49921 tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg tgatagagac
    49981 atagccctca tctaccgcag caaaatcggt ccccggaccg gcatcacatt taccgcccgg
    50041 cgggccaatc cagtacagag tattgccgcg cggatcttgc tgcgggatca cctgatctgc
    50101 cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg atttgatcca agggtaaatc
    50161 cggaacgtta atattaagaa tacgtccggt gcgcagcggc tctttacaca gtgcgcgcaa
    50221 aattgaacag gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc
    50281 aagcgccgga aaacctaaat ggcggccttc catcgcggcg gctaccgtac cggaataaat
    50341 aacatcatcc cccagattcg gcccggcgtt aattccggac acaacaatgt ccgggcgcgg
    50401 acgcatcaga gcattcacgc caagatagac gcaatcggtc ggggttccca tttgcacagc
    50461 aatatcacca ttttcaaagg taaacgtgcg cagggaggat tccagtgtca gagaatttga
    50521 agcgccgctg cggttacgat cgggggcgac cacctgaacg tcagcaaact cacgcaaggc
    50581 tttcgccagc gtttgtatac cgggtgcatg taccccgtca tcattactca gcaatatgcg
    50641 cataatcacc tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa ctacccgccg
    50701 gaagccagaa acggatctct acggtgacgt catcccacca attccagctt aattgttgcg
    50761 gatacagcag catcgctctg cgcgcggctt caactttttc gcgcaccagt aaagcttgta
    50821 attcagtttc tgcggcgacc gctgcttgtt cgaatgccaa cgcttcacgc tgagttcccc
    50881 attcgccact gcccggcaat gcggcggtta tcatcagctc tttatcgttg acgcgacgct
    50941 gtaattccgc cagttcttcg gtggttgcta caaaccagct accacgtccg gctaattgta
    51001 gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt gaggcgctca gcaacaatct
    51061 gattaaacaa cgcactgcgg gctgccgaca accaaaaact ccgtttattg cgatcgcgca
    51121 ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa gttgctaccg ccaatcccaa
    51181 aacgttgggc accgaagtag ttcggtacac cttttacgca aatatcgatc agacgttgtt
    51241 caacgtcatc gcgattgctc acttcgcgca gaaccagggt aaaggcgttt cctttcagcg
    51301 cccctaaacg cagcttgcgc ttgtgccgcg catactccag cacctggcag ccttccagtt
    51361 gaaaggcgct cagatcgggc atttccttgc ccggcacgcg agcgcataac cactgttctg
    51421 taacagcatg tttgtctttt tgcccagcaa agctgacttc acgggcatga attttcagga
    51481 atttcgccag tgcatccgcc acaaaacggg tattgcagcc gtttttgagg attctaacca
    51541 gaatatgctc accttcacca tcaggctcaa agcccaaatc ttccaccacc acaaagtctt
    51601 ccggattggc tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa gtgagattat
    51661 caaactcaat cattttgttg ccttaatgag tagcgccacc gcttcacagg caatcccttc
    51721 cccacgtccg gtaaatccaa gtttttccgt agtagtggct ttcacgttaa catcatccat
    51781 atggcagccg agatcttcgg caataaacac gcgcatttgt ggaatgtgcg gcaacatctt
    51841 cggtgcctga gcgatgatag tgacatcgac gttgccaagg gtataaccct tcgcctgaat
    51901 acgacgccag gcttcgcgta gcagctcgcg gctatcggca cctttaaatg ccggatcggt
    51961 atccgggaac agtttgccga tatcccccag cgccgccgcg ccaagcaatg catcggtcaa
    52021 cgcatggagc gccacgtcgc catcagaatg cgccagcaat cctttttcgt aaggaatgcg
    52081 tacgccacca atgataattg ggccttcacc gccaaaggcg tgtacgtcaa aaccgtgtcc
    52141 aattcgcatt atgtattctc ctgatggatg gttcgggtga ggtaaaactc ggccagtgcc
    52201 aaatcctccg ggcgcgtgac tttaatgtta tccgcacggc cttcgaccaa ctgcggatga
    52261 aatccgcaat attccagcgc cgaggcttcg tcagtaatag tcgcgccttc atttagagcg
    52321 cgcgtcatac agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat
    52381 aagccgttgc gatcaacggt atgagcaatg gcatttttgc ccggttcggc acgtttcata
    52441 gtatcgcgca ctggtgcggc taggatccct cccgtgcggc tggtttcgct caacgccaac
    52501 aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg cgtcatgcac caatacccac
    52561 tgcgcgtcgc cagcggcttt caaacctgcc agcacggaat cggcacgctc atcaccgcca
    52621 tctacaacgg tgatttgcgg atgattcgcc agagaaagtt gtgcaaaacg gctatcgcca
    52681 ggacttatgg caatgacgac acgtttcacc cggggatgcg ccagcagcgc atacaccgag
    52741 tgttcaagaa tggtttgatt accgattgag agatattgct taggacattc cgtttgcatt
    52801 cgacggccaa atccggccgc cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg
    52861 ttaattcccg ggctgattta tcgattgttt tgccccgcag actgtgcgcg cttcgacgcg
    52921 tcaggcacca gacgataaaa agtttcgccc ggcctggtca tgctgagttc attacgcgca
    52981 cgctcttcga gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa aagttgatcg
    53041 tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg ccacatcatc attgacgcgg
    53101 gtatagtcat gtataccgtt cttaccgaac cacagcgaat actgtagcca gaccagaata
    53161 gccagcaaca gcagcgttag tttacccatc ctgccccctg aaaaacggca tcatcatccc
    53221 atgcatccga agacgactct acatcctctg ttggggatac cgcgacaacg cgggcaaatg
    53281 taccacattt gtccattgtt acgtataccc agggcgtgca gaacataatc tcattattag
    53341 ttacggtttg aattatgaac agaggagacg ggaaagtaca aattagccca gtagccacat
    53401 aaacagtgcg ccaaacataa tgcctactgt catcagggtg aaaacaatac tgtagcgtag
    53461 ctttccgtcc atcaatgaat gcagcgcaat ccccaccact accgcgacgg gcatcagcgc
    53521 aagaaagaaa ggccaggtgt agataaagaa gaacagcgtg ttagagccat aaatcaacat
    53581 cggcatcgcc agcgcaaata accaagagat aaaaccgacc acggcaccag gcagtgacca
    53641 tgtggtttct tcatcctcag taaggctgtc gttatttgtt agtgtaatgt tatggctatt
    53701 acgcatattt gatcctgtta ctttgacgaa ccgggcatgg aaacccggtg gtgtctcagg
    53761 atctgataat atcgttctgt ctcaacagat ctaataattg ctgtaccaaa tttgttacta
    53821 attgttcacc attgagatga atttctgccg attcaggcgc ttcgtaaacg gaatctattc
    53881 ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata taagcctttt ggatcgcggg
    53941 cttcgcaaat cgccagcggc gtatcgacaa acacttcgat aaagcgccct tctcctacgc
    54001 gttcacgaac catctggcgt tcggcgcggt gtggcgagat aaatgcggtc agcaccacca
    54061 gtccggcttc aaccatcaaa ttcgccactt caccgacgcg acggatattc tctttacgat
    54121 cggcatcgct aaaaccgaga tcgctgcata atccgtggcg aacattgtcg ccatccagca
    54181 gatacgtact gacgccgagt ttatgtaacg cctcctccag cgccccggcg accgttgatt
    54241 taccggaccc ggagaggccg gtaaaccaca gcactacacc acgatgaccg tggtgtagct
    54301 cgcgttgttg cacagtgacc ggatggctat gccagacgac gttttcgtca tgcagcgcca
    54361 ttatttatcc cccagcaaat cgcgcgcgcc ccagtgtgga aagtggcggc gaaccagggc
    54421 attcaattcc agttcgaatg cactaaattc agatggcgca gcagttgcct ggctaactgg
    54481 ctcatgcacc ataccggcac ctacggtcac attgctcagg cgatcgataa aaatcagccc
    54541 accggtaacc gggttttgct gataacgatc taacaccagt ggctcgtcaa aagtgagatc
    54601 caccaggccg atgccgttca gcggcaggtt ttcaacttcg cgttgggtaa ggttattaat
    54661 atcaacctga taacgaatgc catcaacacg agcacgcgtc ttcttaccgg caattttgat
    54721 gtcgtaactc tggcccgggg aaagcggctg ttccgccatc cataccacat ccaccgacgc
    54781 actctgcacc gccggtaacg cttcgtctgc cgccagcagc agatcaccac ggctgatatc
    54841 gatctcatcc gtcagcacca gggtgatcgc ttctccggca aaggcttctt cgcgatcgcc
    54901 atcaaaagtc acgatccgcg cgacgtttga ttccacacca gagggcagca cttttacacg
    54961 ttgcccgact tccacgcgac cggatgccag cgttccggcg taaccacgaa aatcaagatt
    55021 cgggcggtta acatactgca ccgggaagcg cattggctgg gcatccacca ctcgctggat
    55081 ctccacggtt tccagcactt cgagcagtgt tggaccgctg taccacggca tactttcact
    55141 ttgcgaagcc acgttgtcgc cttccagtgc agagagcggc acaaagcgga tatccagatt
    55201 acccggcagc tgcccggcaa aggtcaaata atcttcacga atacgggtga acgtctcttc
    55261 actgtaatcc atcagatcca ttttgttgat cgccacgacc agatgtttga tccccaacag
    55321 tgtggagata aaactgtgac gacgggtttg atcgagcacg cctttacggg catcgatcag
    55381 taagatcgcc agttcacatg tcgatgcgcc agtcgccata ttgcgggtgt actgctcgtg
    55441 tcctggggtg tcggcgataa taaatttacg cttctcagta gagaaatagc ggtaggccac
    55501 atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg tccaccagca gtgccagatc
    55561 cagcttttcg ccctgggtgc cgtgacgttt actgtcgtta tgcagcgatg agagctgatc
    55621 ttcgtagatt tggcgggtat cgtgcagcag gcgaccaatc agggtacttt tgccgtcatc
    55681 gacgctacca caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc
    55741 ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc
    55801 cctgacgttt tttcagctcc atagaccccg cctggtcgcg gtcaatcacg cggccctgac
    55861 gttcactggt ggtggaaacc agcatctctt cgatgatctc cggcagtgtt tgtgcatttg
    55921 actccaccgc accggtcagc ggccagcagc ccagcgtacg gaaacgcacc atccgttttt
    55981 taatcacttc gcccggttgc aggtcgatac ggttgtcatc aatcatcatc aacataccgt
    56041 cgcgttccag aaccggacgt tccgcagcga gatacagcgg cacaatgtcg atattttcca
    56101 gccagatgta ttgccagata tcctgctcgg tccagttaga gagcgggaag acgcggatgc
    56161 tttcgccttt gttaatttgc ccgttgtagt tgtgccacag ctccgggcgc tgatttttcg
    56221 gatcccagcg atggaagcgg tcacggaaag agtagattcg ctctttagcg cgggatttct
    56281 cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc gtatttgttc agcgcctgtt
    56341 tcaggccttc agttttcata atatcggtat gtttcgcgct gccgtgcacg aatggattaa
    56401 tccccatcgc cacgccttcc gggtttttat gcaccagcag ctcgcagccg taggctttcg
    56461 ccgtacgatc gcggaactca tacatctcgc ggaatttcca gccggtatcg acatgcagca
    56521 acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg
    56581 aatctttacc gatagagtag agcatcaccg gatttgagaa ttctgccgcc acctcgcgaa
    56641 taatgtggat actttccgcc tccagttgcc gcaggtgagt aagtcgtttt tgatccataa
    56701 ccgttccttt gcaataccgc tattttcttg ccatcagatg tttcgactat agggagcgta
    56761 agagaacgaa tgaaattacc aattagaatg agtagttcct taacggaata acgatttggc
    56821 aaagctaata tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga agatttgaaa
    56881 ttgttttagc gcagcggcag tttcatacta tggcggtaaa aaaatttgca tggtatttaa
    56941 ggactcacta tgttttccgc attgcgccac cgtaccgctg ccctggcgct cggcgtatgc
    57001 tttattctcc ccgtacacgc ctcgtcacct aaacctggcg attttgctaa tacccaggca
    57061 cgacatattg ctactttctt tccgggacgc atgaccggaa ctcctgcaga aatgttatct
    57121 gccgattata ttcgccaaca gtttcagcaa atgggttatc gcagtgatat tcggacattt
    57181 aatagtcggt atatttatac cgcccgcgat aatcgcaaga gctggcataa cgtgacggga
    57241 agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc agatcatcat tatggcgcat
    57301 ctggatactt acgccccgct gagcgatgct gacgccgatg ccaatctcgg cgggctgacg
    57361 ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca tgctggaatt ggcagaacgc
    57421 ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg cgaccagcgg cgaagaggaa
    57481 gggaaattag gcgctgagaa tttactcaag cggatgagtg acaccgaaaa gaaaaatacg
    57541 ctgctggtga ttaatctcga taacttaatt gttggcgata aattgtattt caacagcggt
    57601 gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca gggcgctggc aattgcgcgt
    57661 agtcatggaa ttgccgcaac gaccaatccg ggtttgaata aaaattatcc gaaaggcact
    57721 gggtgttgta atgacgcaga aatattcgac aaagcgggca ttgctgtact ttcggtggaa
    57781 gcgactaact ggaatcttgg gaataaggat ggttatcagc aacgcgcaaa aacagccgca
    57841 ttccctgcgg gaaatagctg gcatgacgta agactggata atcaacagca tattgataag
    57901 gcacttcctg gaagaataga acgtcgctgc cgtgacgtta tgcggataat gctaccactg
    57961 gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat gggagctggg tgttctaccg
    58021 caggggcggg gaattctaag tgatatccat catcgcatcc agtgcgcccg gtttatcccc
    58081 gctggcgcgg ggaactcgag acctgccatg aggtggattt atcagtcggc ggtttatccc
    58141 cgctggcgcg aggaactctg cacgaaaaat ggcaggcttg ccagcatcct cggtttatcc
    58201 ccgctggcgc ggggaactcg acccacgcac acactgcgcg cagccctgat tcggtttatc
    58261 cccgctgacg cggggaactc gattttttcc gcttctgaca aatggttttt atcggtttat
    58321 ccccgctggc gcggggaact ccccgaaccc ggcaacatgc tcgccgacct gcgcggttta
    58381 tccccgctgg cgcggggaac tcttgatgaa gcggcgcgtg ttatagctga tggcc
//
LOCUS       DAZUGK010000006        54852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZUGK010000006 DAZUGK010000000
VERSION     DAZUGK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.006"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..163
                     /gene="yhfL"
                     /locus_tag="ACHY5W_000304"
     CDS             <1..163
                     /gene="yhfL"
                     /locus_tag="ACHY5W_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFS4445272.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            complement(270..1355)
                     /gene="yhfS"
                     /locus_tag="ACHY5W_000305"
     CDS             complement(270..1355)
                     /gene="yhfS"
                     /locus_tag="ACHY5W_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4445273.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHARQQPQDSYVLADVLATL
                     RLAGIPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAHALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESISSV"
     gene            complement(1367..2670)
                     /gene="yhfT"
                     /locus_tag="ACHY5W_000306"
                     /pseudo
     CDS             complement(1367..2670)
                     /gene="yhfT"
                     /locus_tag="ACHY5W_000306"
                     /inference="COORDINATES: protein motif:HMM:NF022254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
     gene            complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHY5W_000307"
     CDS             complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHY5W_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFS4445274.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHY5W_000308"
     CDS             complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHY5W_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFS4445275.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYSFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSCYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHY5W_000309"
     CDS             complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHY5W_000309"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4445276.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFITHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVTTQIGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5148..6311)
                     /gene="yhfX"
                     /locus_tag="ACHY5W_000310"
     CDS             complement(5148..6311)
                     /gene="yhfX"
                     /locus_tag="ACHY5W_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFS4445277.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(6395..6757)
                     /gene="yhfY"
                     /locus_tag="ACHY5W_000311"
     CDS             complement(6395..6757)
                     /gene="yhfY"
                     /locus_tag="ACHY5W_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4445278.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVKVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWIAANEEV"
     gene            complement(6774..7679)
                     /gene="yhfZ"
                     /locus_tag="ACHY5W_000312"
     CDS             complement(6774..7679)
                     /gene="yhfZ"
                     /locus_tag="ACHY5W_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFS4445279.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTINELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7948..8952)
                     /gene="trpS"
                     /locus_tag="ACHY5W_000313"
     CDS             complement(7948..8952)
                     /gene="trpS"
                     /locus_tag="ACHY5W_000313"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4445280.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8945..9703)
                     /gene="gph"
                     /locus_tag="ACHY5W_000314"
     CDS             complement(8945..9703)
                     /gene="gph"
                     /locus_tag="ACHY5W_000314"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFS4445281.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9696..10373)
                     /gene="rpe"
                     /locus_tag="ACHY5W_000315"
     CDS             complement(9696..10373)
                     /gene="rpe"
                     /locus_tag="ACHY5W_000315"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4445282.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10391..11227)
                     /gene="dam"
                     /locus_tag="ACHY5W_000316"
     CDS             complement(10391..11227)
                     /gene="dam"
                     /locus_tag="ACHY5W_000316"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS4445283.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11334..12620)
                     /gene="damX"
                     /locus_tag="ACHY5W_000317"
     CDS             complement(11334..12620)
                     /gene="damX"
                     /locus_tag="ACHY5W_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFS4445284.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNTTDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12712..13800)
                     /gene="aroB"
                     /locus_tag="ACHY5W_000318"
     CDS             complement(12712..13800)
                     /gene="aroB"
                     /locus_tag="ACHY5W_000318"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4445285.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGDAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13857..14378)
                     /gene="aroK"
                     /locus_tag="ACHY5W_000319"
     CDS             complement(13857..14378)
                     /gene="aroK"
                     /locus_tag="ACHY5W_000319"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFS4445286.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14779..16017)
                     /gene="hofQ"
                     /locus_tag="ACHY5W_000320"
     CDS             complement(14779..16017)
                     /gene="hofQ"
                     /locus_tag="ACHY5W_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFS4445287.1"
                     /translation="MKQWIAALLLMLIPGVQAVKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWAAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15929..16333)
                     /gene="hofP"
                     /locus_tag="ACHY5W_000321"
     CDS             complement(15929..16333)
                     /gene="hofP"
                     /locus_tag="ACHY5W_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFS4445288.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16323..16763)
                     /gene="hofO"
                     /locus_tag="ACHY5W_000322"
     CDS             complement(16323..16763)
                     /gene="hofO"
                     /locus_tag="ACHY5W_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFS4445289.1"
                     /translation="MNMFFDWWFAISPRLRQFCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16747..17286)
                     /gene="hofN"
                     /locus_tag="ACHY5W_000323"
     CDS             complement(16747..17286)
                     /gene="hofN"
                     /locus_tag="ACHY5W_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFS4445290.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17286..18065)
                     /gene="hofM"
                     /locus_tag="ACHY5W_000324"
     CDS             complement(17286..18065)
                     /gene="hofM"
                     /locus_tag="ACHY5W_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFS4445291.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            18185..20737
                     /gene="mrcA"
                     /locus_tag="ACHY5W_000325"
     CDS             18185..20737
                     /gene="mrcA"
                     /locus_tag="ACHY5W_000325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFS4445292.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVKIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20902..21462)
                     /gene="nudE"
                     /locus_tag="ACHY5W_000326"
     CDS             complement(20902..21462)
                     /gene="nudE"
                     /locus_tag="ACHY5W_000326"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFS4445293.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21782..23917
                     /gene="igaA"
                     /locus_tag="ACHY5W_000327"
     CDS             21782..23917
                     /gene="igaA"
                     /locus_tag="ACHY5W_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFS4445294.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFVDAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVATFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TETVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            23982..24650
                     /gene="yrfG"
                     /locus_tag="ACHY5W_000328"
     CDS             23982..24650
                     /gene="yrfG"
                     /locus_tag="ACHY5W_000328"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFS4445295.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24661..25062
                     /gene="hslR"
                     /locus_tag="ACHY5W_000329"
     CDS             24661..25062
                     /gene="hslR"
                     /locus_tag="ACHY5W_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFS4445296.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFTHGDSE"
     gene            25087..25965
                     /gene="hslO"
                     /locus_tag="ACHY5W_000330"
     CDS             25087..25965
                     /gene="hslO"
                     /locus_tag="ACHY5W_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4445297.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26028..27752)
                     /gene="yhgE"
                     /locus_tag="ACHY5W_000331"
     CDS             complement(26028..27752)
                     /gene="yhgE"
                     /locus_tag="ACHY5W_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFS4445298.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALVVLVLLVWSLGY
                     FVSILWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPGQVTIYMLEQSGHYGRAALESLKSDAGFMKDPKRAWDLLMALDGEQSLQKVVSEKS
                     LAENVLIAPGSGKPDAAFWSALIKERYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            28131..29753
                     /gene="pckA"
                     /locus_tag="ACHY5W_000332"
     CDS             28131..29753
                     /gene="pckA"
                     /locus_tag="ACHY5W_000332"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4445299.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVNPVSKAGHATKVIFLTADAFAVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29829..31181)
                     /gene="envZ"
                     /locus_tag="ACHY5W_000333"
     CDS             complement(29829..31181)
                     /gene="envZ"
                     /locus_tag="ACHY5W_000333"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFS4445300.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31178..31897)
                     /gene="ompR"
                     /locus_tag="ACHY5W_000334"
     CDS             complement(31178..31897)
                     /gene="ompR"
                     /locus_tag="ACHY5W_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFS4445301.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32125..32601
                     /gene="greB"
                     /locus_tag="ACHY5W_000335"
     CDS             32125..32601
                     /gene="greB"
                     /locus_tag="ACHY5W_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFS4445302.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32698..35019
                     /gene="yhgF"
                     /locus_tag="ACHY5W_000336"
     CDS             32698..35019
                     /gene="yhgF"
                     /locus_tag="ACHY5W_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4445303.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLANLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKELMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35457..35684
                     /gene="feoA"
                     /locus_tag="ACHY5W_000337"
     CDS             35457..35684
                     /gene="feoA"
                     /locus_tag="ACHY5W_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFS4445304.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35701..38022
                     /gene="feoB"
                     /locus_tag="ACHY5W_000338"
     CDS             35701..38022
                     /gene="feoB"
                     /locus_tag="ACHY5W_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFS4445305.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNFVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38022..38258
                     /gene="feoC"
                     /locus_tag="ACHY5W_000339"
     CDS             38022..38258
                     /gene="feoC"
                     /locus_tag="ACHY5W_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFS4445306.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            38461..39339
                     /gene="rpnA"
                     /locus_tag="ACHY5W_000340"
     CDS             38461..39339
                     /gene="rpnA"
                     /locus_tag="ACHY5W_000340"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFS4445307.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRTIAKTMLDAGVPLEDV
                     LRFTGLSEEELAAASQ"
     gene            39411..39680
                     /locus_tag="ACHY5W_000341"
     CDS             39411..39680
                     /locus_tag="ACHY5W_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4445308.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLATDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDENEIKALNQSSEGWAEEKKGNEAW"
     gene            39674..40042
                     /locus_tag="ACHY5W_000342"
     CDS             39674..40042
                     /locus_tag="ACHY5W_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4445309.1"
                     /translation="MVKARTPHRGEIWCFNPDPVAGHELRGPHYCIVVTDKELNNVLK
                     VAMCCPISTGANAARSSGVTVNVLPHDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEALISEVIGKLVNLIDPQQ"
     gene            complement(40057..40827)
                     /gene="bioH"
                     /locus_tag="ACHY5W_000343"
     CDS             complement(40057..40827)
                     /gene="bioH"
                     /locus_tag="ACHY5W_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFS4445310.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            40865..41548
                     /gene="gntX"
                     /locus_tag="ACHY5W_000344"
     CDS             40865..41548
                     /gene="gntX"
                     /locus_tag="ACHY5W_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFS4445311.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41607..42182
                     /gene="nfuA"
                     /locus_tag="ACHY5W_000345"
     CDS             41607..42182
                     /gene="nfuA"
                     /locus_tag="ACHY5W_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFS4445312.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42543..43859
                     /gene="gntT"
                     /locus_tag="ACHY5W_000346"
     CDS             42543..43859
                     /gene="gntT"
                     /locus_tag="ACHY5W_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS4445313.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43904..45988)
                     /gene="malQ"
                     /locus_tag="ACHY5W_000347"
     CDS             complement(43904..45988)
                     /gene="malQ"
                     /locus_tag="ACHY5W_000347"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFS4445314.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTVLRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45998..48391)
                     /gene="malP"
                     /locus_tag="ACHY5W_000348"
     CDS             complement(45998..48391)
                     /gene="malP"
                     /locus_tag="ACHY5W_000348"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFS4445315.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49003..51708
                     /gene="malT"
                     /locus_tag="ACHY5W_000349"
     CDS             49003..51708
                     /gene="malT"
                     /locus_tag="ACHY5W_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFS4445316.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            51775..52053
                     /locus_tag="ACHY5W_000350"
     CDS             51775..52053
                     /locus_tag="ACHY5W_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFS4445317.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            52044..52529
                     /locus_tag="ACHY5W_000351"
     CDS             52044..52529
                     /locus_tag="ACHY5W_000351"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4445318.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSTGLSRV
                     YVICIVNTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(52579..53607)
                     /gene="rtcA"
                     /locus_tag="ACHY5W_000352"
     CDS             complement(52579..53607)
                     /gene="rtcA"
                     /locus_tag="ACHY5W_000352"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFS4445319.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQVGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFPL
                     HEQYIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLQVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(53611..54837)
                     /gene="rtcB"
                     /locus_tag="ACHY5W_000353"
     CDS             complement(53611..54837)
                     /gene="rtcB"
                     /locus_tag="ACHY5W_000353"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFS4445320.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13107 a        15112 c        13772 g        12861 t
ORIGIN      
        1 caaatttatt aaagttgcac tggtaggtgc ggtactggct acgttaactg catgtactgg
       61 tcatattgaa aaccgtgata agaactgctc ttacgactac ctgctgcacc cggcaatttc
      121 tatttctaaa atcattggcg gctgcggtcc tactgcacag taagtctctg actgaaatta
      181 gctaacgctg atttaattgc ctgacgccct cgggattacc cgagggcgta tttgtttctg
      241 cattcttttc gaaaatatcg ttcaaaagat taaacgctgc taatactctc acgcaaaatc
      301 cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca atgttcgagt
      361 tgcggattcg cctggcggaa cgttccggaa agtcgataaa aaagcggcgg gatttcatat
      421 ttcgactctg cacccactgg gtaaggcaag gcaccgcgct tttgcgcctc ttccagcacc
      481 ctggcggcaa tcggctgatg aaactcgaca atcaacacct tcgactgcgc attggcaatg
      541 acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa ccgttcggat
      601 actcccgcct gcaccgcgtg catcactggc gcgaacacca gcccacgcaa cacttccaac
      661 gcatgagcgc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat ccgactaata
      721 acatcggcat cgccaaccac tgcaccaacg ccctccggcc caaacagctt gaagcaggag
      781 aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ctaccttcat caccgcatag
      841 ttgtcatcgg ttaacgctgg aatgccagcc aggcgcaacg ttgccagcac atctgccagc
      901 acgtagctgt cctgcggctg ctggcgcgca tgctgcacca gcgccgcatc cggttgttgc
      961 tcgtcgacga cctgcttcag tgccgacagg tcattgaaat cagcagtaat aagcgtcagc
     1021 cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg caccagaaga
     1081 cgctgccccg gtttgagcaa agccgccagc gccgcacgaa tcgctccagt acccgcgccc
     1141 tgtaccagcg ccgcggcctg tgcgtgaaat gcatcagcca gcacctgctc cacccgttgg
     1201 gtaatacgcg gttgattcag acctggagtt aaccctaaat caccgccagt aagaaactcg
     1261 ctgccgggga aatggcgaca aatgctatcc accagcgcaa actgcttttg ctgcgcctca
     1321 ataatcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa gccggaacaa
     1381 acagacctag ccagtaaaga acattcagca ggatgccggt gatcattact gctacgaccg
     1441 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
     1501 cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa ccgaccagca
     1561 gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
     1621 acgggtagcg tcccagccat ttgccgatag aacgaagcag caagacttcc gccgaaataa
     1681 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
     1741 caaaggtaaa gcccgcaact gcatacacac ccgttgctaa ggcggtggtg gcaatcaacg
     1801 gtacaaaacc cagcccgcgc ataaattctg ccagtgcagc ctggttgatc agcgtttgcg
     1861 attgttccgg cgttacgcct gcggagtacg ctttctccag agtgaagatc gacacttcac
     1921 tgccggcgaa aatcttcata ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
     1981 agggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc ccgctggcat
     2041 cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
     2101 ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacta
     2161 ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag ccaaactggt
     2221 agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag cttaattccc
     2281 cgaggctacc taatacatcc accggcagcg cggtcagcag ctggtttact ggcaacaggc
     2341 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
     2401 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttagca gcccggtttt
     2461 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
     2521 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc
     2581 gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     2641 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     2701 agcatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac ttttccttca
     2761 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     2821 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     2881 cacgcgccga tgtaataatc cagttgcccg gatttcacct tcattgccgc ttccatgtcg
     2941 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatagtttt tttaatctgc
     3001 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     3061 agagggattt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     3121 ttgcggaata aaggtggtta gtaaatagtc atagccataa ccaccgttgg cttttaaatg
     3181 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcgcgtag
     3241 cgcatgaagc atcgcaatac gcttttcgtc cgggtagcaa ctgttcttac caatggtgtc
     3301 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tatctttcag
     3361 atcgcagtga ccaacggtga cacgcgaaag atcaactccg tgggcttgca acaaagccag
     3421 ttgctccagc cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt
     3481 atgtgccagc gcggcggcaa taaatacctt ctcttccagc ggcgtaatct tcccttcgct
     3541 ggagccgatc tccgcgatga tcccggcttt cagttccgtg ccatcgatac cctgctcaat
     3601 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa
     3661 aaacgcgtcc tggtaataac cggtacaggc caccacgttg atccccgtct cgcgcattac
     3721 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     3781 cacgccccgg gccatcaggt cgttcatctc ctggcaaatg aacgaatact gatcaaggcg
     3841 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt
     3901 gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     3961 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca
     4021 gaaagcgtgg tgcgcacacc gatctgcgta gtgaccagcc cttgctgata aaccagcact
     4081 ggcaccactt cacgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
     4141 atcacgacaa ggcaatcatc tggctgcatc gcctcgacaa gccgggcaag attacggtca
     4201 acgacctgca aacgttcggc ataacgtgcg acgtcttctg cgtgaccagc gaggtcggtt
     4261 tcctgaatgt tggtgcaaat aaacgctgtc tgatgggtga taaattcgtc gagggtgata
     4321 tccataatcc gctggctatc caccagattt tgccagctca cgccataagg attgttgacg
     4381 atatctgcca ccttacccac cagcacggtt ggcacgcctg cttcatacag tttttgtggc
     4441 acctgcactt tttcatcgac gccatagccc atatgcacga cctggaaacc gttgtcataa
     4501 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
     4561 agaatacgct gactgtcggt taacaggccg ccaaatgtaa tgacccggcc gacctgtacc
     4621 tggtcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
     4681 gcggtaatgt tatagacttg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     4741 tgattgaccc acagaaattg cagttcatcg ccacggcgct ccacctgcca accggcggat
     4801 actaatgcct gctcaacacg gtcaatcaca tcgcgaaagg gcatccgcag cggcggtaac
     4861 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
     4921 tctgccacgc cccaggttgc ggagtctgac ggctgcatat cgcctggcgc ataacccaat
     4981 gcgttgatta gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg
     5041 atgtgaccac aggtattcgc cccggcatcc tgcggacgca ccaacgtgac atctttcatt
     5101 gcccctacgc caaagctatc gatcactaac accacaaatc gcgccattta cgcctccaga
     5161 gggttgccga gactgtcata acgaccaacg attttcggtt cgccacggtg aataccggac
     5221 accagcacca catcgctacg ggtgacaaaa atctgcgtgc ggaaacagag caccactgca
     5281 ctgcttaccg gaaactcgcc tgccagcgac agggtgtagt cgatactgct gtcatccaca
     5341 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
     5401 gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
     5461 gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg ctggtttgcc
     5521 ggaatagtac cggtcagcgc atgaccgggt tcggcatgag tcgcaccgta ttccgccagc
     5581 aatggcagcg aagtgcagct ggtcgctgaa ggcgcgttca gttgctcaat cgcaatacca
     5641 gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt tggcaaaact
     5701 ttcccggcag cctcatccca aagcaggcaa gggaaatggg ttagtccggc taaatgcagc
     5761 cccggcagtt tcttaatttc agcaaccacc tcatgcagcg aatgctgtac aaaaccgctc
     5821 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac agactggatt
     5881 cgcccggcct tcaccgccgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
     5941 atgacgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat ttgtaccaga
     6001 tgcccctgat gcgccaccgg caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
     6061 accgccacaa tgccgctgta gcccaacgcc agcagttttt ccgccagcca tgggttacga
     6121 ccgaattgtt tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatcagc
     6181 cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg ggcgatcttg
     6241 ccctgctgcc acaggcttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
     6301 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
     6361 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagctatcc
     6421 ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact tccagtgcga
     6481 attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
     6541 gctctgccag caactcgtta tccagtggct caatttcttc accacggcga ctgcgcatca
     6601 gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggcagatggc
     6661 actctgtttc cagtaccttg acgacctgca tcatgccttt gcagatgtcc ttatcaataa
     6721 cgcctgcatc gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa
     6781 ctcggttcct gttccccact caccacccgt tgttgatggg cgagcagggc gtgtttatct
     6841 acaacggcac gcagcagttg ttgcattggg taatcatcga ctcgcgtcag gaccacggct
     6901 tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcctctaa tcccagcatg
     6961 gtcagttcgt tttccgccac cacgttccag ataaccgcat caacatcgcc tttaacaatg
     7021 cgttgtaaac tctcgtgata agagagatcg actcgttcca catcactacc gccaaaaaag
     7081 acatcagtca tgattttctg atccgccgaa cggttatcca gccccacgcg cttcacgttt
     7141 gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg cggccccaac
     7201 tccagcgcga ggcataaccc tttttgcgtg agataacttt ccgccgccag tcgggaaacc
     7261 accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
     7321 tgcgcatagt aaaaaggaat gccatcaaac tgggctttca atccgctcgc taaaccttcg
     7381 tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc cacatgactc
     7441 agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg gcgttcaatc
     7501 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aacggatgaa
     7561 cggcattcag tggccagttc atttatagtt ttcagtcgat taccgcactt ttcacccaac
     7621 aaataacggg ccagcgtcgt gataacgacg ccttcttttt tgataaacgt tcgacgcata
     7681 acatattttc aataaaatga atgtttatat cttcattaaa atgaagataa gtggcaactg
     7741 tgagatgtgg aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa tgaggataaa
     7801 aaaaccgggt ttccccggtt tcagagtgat gacgaagtga tttattcgac aatgcgagca
     7861 aagggtaatt cccactggcg cttgcctgct cccctcaccc taaccctctc cccaaagggg
     7921 cgaggggacc gtccgagcgt tttgatatta cggcttcgcc acaaaaccaa tcgcttcata
     7981 caccgctttt agcgtacggg aagcgtgcgc gctggctttt tccgcgccat ctttcatcac
     8041 ctgttgcagg aaggcttcat cgttgcggaa acggtgatag cgttcctgca attcagtcag
     8101 cataccggaa acggcatcag ccacttcacc tttcagatga ccatacatct tgccttcgaa
     8161 ctgtttttcc agttctggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt
     8221 ggaaacgccc gctttgttct gcacatcgta gcgaactacc ggcggctcgt cggagtcagt
     8281 gaccgcgcgt ttgattttct tcactaccga tttcggatct tccagcaggc cgataacgtt
     8341 attgcggtta tcgtcagact tggacatctt cttggtcggc tccagcagcg acattacgcg
     8401 cgcgccagat ttcggaataa acggctccgg caccttaaag atctcgccat acagcgcgtt
     8461 gaaacgctgg gcaatatcgc ggctcagttc gaggtgctgt ttctggtctt cacccaccgg
     8521 taccagatta gtttgataca gcaggatgtc cgcagccatc agcaccggat agtcaaacag
     8581 accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcggct
     8641 cagttcgccg aagtaggtat aacagttcag tgcccaacct aactgtgcat gttccggcac
     8701 gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc
     8761 cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg
     8821 ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc actgacgcag
     8881 cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac taaaaacgat
     8941 gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa
     9001 ggtcatttat agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt
     9061 tatatccgta ggttaagcca actgatgggc aacctgccgc ttttgctgcc tgaatatcat
     9121 tgcgtgagtc gccaacaaac agcatctgtt gtggagcaat tcccatccgc tcagccacca
     9181 gtaacagcgg gtccggatgc ggttttttgt tttgcacatc atcaccacca atcaccacgc
     9241 tgaagtattt ggcgatatct aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt
     9301 tggtgaccag gcctagcggc aggcctttag cctgcaacgc gcccaacgta tcggcaacgt
     9361 gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata atagcgatcg aacagtttac
     9421 gcagaatacg tacctgttct tctgccggaa tgtcgtcatc aacgggcggt ttacccattg
     9481 ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat
     9541 ctgcgccgtt accaatccag gtaataacac gttcttcacc tgcaacgggc aactccagcg
     9601 catacagcgc catatctacc gcagcagcaa gaccgggagc actgtccacc agcgtaccgt
     9661 cgagatcaaa agcgacgccg cgaatatctt caaacttact catgacttac ctttgccagt
     9721 tcactgcgca tttcatcaat gacttttttg tagtctggct ggtcgaagat tgccgaaccg
     9781 gcaacgaaca tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac cttcacgcct
     9841 ccgtccactt ccagacgaat gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc
     9901 agtttatcca gtgtttgagg aatgaaagac tgaccgccga aaccaggatt gacggacatc
     9961 agcaggatca catccagctt atccatcacg taatccagat agctcagagg tgtcgccggg
    10021 ttaaatacca gacccgcttt acagccattt tctttaatca gttgcagcgt gcggtcaaca
    10081 tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc
    10141 acaatgcgat cgacgggttt caccatcaga tgtacgtcga taggggcggt aatgccatag
    10201 ttacgcaagg atttcagcac cattggccca atcgtcagat tgggaacata gtggttatcc
    10261 atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc
    10321 aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc
    10381 tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt
    10441 cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat
    10501 gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca
    10561 caggaatatg gcgatcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa
    10621 gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggtgcataag
    10681 gcggatcgca atagacgaca gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact
    10741 cacaatagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggaa
    10801 aatagggttt tttgtagcgg ccgaacggca cgttaaactc accgcgcaga ttgtaacgac
    10861 acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat
    10921 cctggctttt gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg
    10981 gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat
    11041 agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga
    11101 gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc
    11161 gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt
    11221 tcttcatgct gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc
    11281 cagcgcttca ggcaactccc gcaacgataa accattgcgg gagataacct tgattacttc
    11341 agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg aacatctgct
    11401 ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg
    11461 acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct
    11521 ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa
    11581 tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc tccagcggct
    11641 ggtgttgccg tggtttgcgc cggggatgcc gtttgtactg gagccgtagt cgccgtctct
    11701 tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc tcgccacagg agcagctggt
    11761 tcagtacgct tcggcgtctg tgctaccggc ttcggttccg ttttcacggt tgcttgcggt
    11821 tttttcggtt caatcaccgc ctgctgacga gctggacgcg tagtggccgg acgttcagcg
    11881 gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gtactggcgc gacagttgct
    11941 ggttcagtcg gcaatgtaga attgaccgcc acattgttca actgttgctg attttgtggc
    12001 tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc
    12061 gccggggttt gcccttgagt cggcgtagaa gagatcggcg gcagagaaac atcctgctga
    12121 gtattttccg cagacgtggt tcccggcgct ggctgcacgc cattggcctg atcggtcgta
    12181 ttgccagcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga agaggtcgag
    12241 ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag aatgccgacg
    12301 cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga
    12361 cgacgctcta cgcgctcttc atccacagtt tcatcttcgg attcgtcaat ttcttcttcg
    12421 atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca
    12481 tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt
    12541 tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct
    12601 tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa
    12661 ttaacctgaa gctaatagac cacttgataa gcggcctgac ctttcttgtt gttacgctga
    12721 ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact
    12781 cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg
    12841 cagcatatgc ggtaaatacg cctgcgcgga catttcgcgc ggcccattga ccggtaaccc
    12901 agcccgcgtg agcagggtta taatacgctg cgtttcggca gaactaaact gcccgagacg
    12961 ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcgtcaccat gtaaccaatt
    13021 gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc
    13081 acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg
    13141 gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc
    13201 cagccagtta aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc
    13261 cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc
    13321 aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc
    13381 gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc
    13441 gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc cgccaagcgc
    13501 caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag
    13561 tacagccagg cttttatact gctcgccgtc agggaggata acgctatcga cgttaacacc
    13621 cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgttggt
    13681 gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa
    13741 accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat
    13801 aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag
    13861 ttgctttcca gcatgtgaat aatctggtta gcgaccactt tagcgctttg atcatcagta
    13921 cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc
    13981 agaacttcac gtggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg
    14041 cgtgcaagtt gcttttcgat cgtcgtttca agataaacga caacgccacg agcggaaaga
    14101 cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt
    14161 ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct
    14221 aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg
    14281 taaaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc
    14341 ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta
    14401 ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca
    14461 taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg
    14521 aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc
    14581 gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga
    14641 atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact
    14701 ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat
    14761 taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa
    14821 ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc
    14881 gccaagcaac ggcacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag
    14941 cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc
    15001 cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa
    15061 tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc ccaggacggc
    15121 ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc
    15181 gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactagcgat
    15241 aatatccagc tgctgttttt gttcgagcgc agaaagctca agatccagca aacgtccgtt
    15301 gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctgcc
    15361 aagcgtggtg acttgcccaa cgccaccagc ttgttgcgca tcggccagcg tccatttcac
    15421 gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg
    15481 cccgaccggc agatccattt gcgctgccca ctgttcaagg gcgcttaacg ccgttttgtt
    15541 atctcgtagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag
    15601 tagcttttcc ccagctttcg ccagctctcc ggcgtcggcg tattgcagag taatattgcg
    15661 attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcttcctgac gggcgatatt
    15721 gtcattctgc caggcaatgg aatgcactga gagaatgttg ccctcctggc gcgttatcag
    15781 tccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa
    15841 cgacaccgta ccgctgacgt ctggcgacac caccaggttc aacttctcct gttcagccag
    15901 cgcctgcaac acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt
    15961 taccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg
    16021 tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg gtacccagcg
    16081 ttagtacgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcattttgct
    16141 gtacccgtcg ccatttcttc tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc
    16201 ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcataga tcttccggcg
    16261 gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc
    16321 gtttaaccct catgcggcgt ctccagttgt agcgtgaaca aaaggtcatc accttccacg
    16381 cttaacgaaa aacggctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc
    16441 actgcttccc acagcgtttt caacgccaac tcacctccct gcgcggatgg atgccaggaa
    16501 accagttgcg cgccggataa ctgaaaatcc agaggcgaaa agggcagcgt tttttcctcg
    16561 ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg atgacttgcc
    16621 cgtagtcgaa ttaatgcgtc gctctcttca tggtgtgtcg atgacagaaa aatgagcgta
    16681 actaacatca gcaaccagac tgcccagcaa aactggcgga ggcggggtga tatggcgaac
    16741 caccagtcaa agaacatgtt catcgctaac cttccttgtt aactgatact caaattgcca
    16801 gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg agatgaaaag atgcatcctg
    16861 gcggagtgac gtttctagtg cgtttaacgc ggtaatgctt gttgtcagcc ccttgatctc
    16921 cagcgttccc tgctgccagc ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc
    16981 cagtgcttcc agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct gcctttgcaa
    17041 acgctgctca cgaatttgtt ggcgctccag caaacgtggt ttcgttatct gtaagctgcg
    17101 ggcgagttgt tgttccccct gaagcaaaac ggcgtctacg cgagcttcgg cgctgcttgt
    17161 cagacgcagt attagcgtta tcccgacggc cagcagcaga ggcgcaacga acatcagcaa
    17221 ccagaaacga agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg
    17281 gttcatcagt acgccttccc aagcgccagc cccagcgcga tggcaaagtc tccacctggc
    17341 ggcggtaacg gcggctgacg aacagaaacg gcctcccaag gatcaaatcc gccttcgcca
    17401 catatcgcga cgctttcggg atcaacggat aacgctgccg ccagctcctt cgcgctagtc
    17461 atccccaccg ccagtttgcg cccccagcga tagcgtgtcg cccacagcca ctgttcgtta
    17521 tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg ctgtaatgca
    17581 ctggcatccg gggtgatcgc gctcacatga acacgcaacc tttctgccag agtgagtagc
    17641 gttgccagct ctttgctttg cgcggcagtc acgttataag cgggactgag tgagtcttcg
    17701 ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgggccat cgtcccggac
    17761 agccaggcca tttgctcccg ctcaccaagg gacatcgacg ggcgcggaaa tgaccgctgt
    17821 aatgtgcgac tggcgggaaa cgccaacata atgtgatgac gctgcggcag ttcgcgactc
    17881 cacggtaaca acgttttagc cagccgctgc gcatcaacaa tccgcccatc tttgataatg
    17941 tcgtgcgcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgc gccccgcaca
    18001 atagcaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca gatcttaaat
    18061 gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat gcgtctggct
    18121 tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa atgggaaatt
    18181 tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc tgctgggagc
    18241 aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg tggcgacatt
    18301 aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg agctgattgc
    18361 tcaatacggt gagaaacgtc gtattccggt tacgctggat caaatcccac cggagatggt
    18421 gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg ttgacccggt
    18481 ggggatcttc cgtgcagcaa gcgtggcgct gttctccggc cacgcgtcac aaggggcgag
    18541 taccattacc cagcagctgg cgagaaactt cttcctaagt ccagaacgca cgctgatgcg
    18601 taagattaag gaagtcttcc tcgcgattcg cattgaacag ctgctgacga aagacgagat
    18661 cctcgagctt tatctgaaca agatttacct tggttaccgc gcctatggtg tcggtgctgc
    18721 ggcacaagtc tatttcggaa aaacggtcga ccaactgacg ctgaacgaaa tggcggtgat
    18781 agccgggctg ccgaaagcgc cttccacctt caacccgctc tactcgatgg atcgtgccgt
    18841 cgcgcggcgt aacgtcgtgc tatcgcggat gctggatgaa gggtatatca cccaacaaca
    18901 gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg agattgcttt
    18961 ttctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt atggcgaaag
    19021 tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc agcaggccgc
    19081 acagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct atcgcggccc
    19141 ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga ttaccgatac
    19201 gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca gcgccaatcc
    19261 tcaggaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta tggaaggcgt
    19321 tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc gtaaagtgac
    19381 cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg catggtggct
    19441 ggcacaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa atggtgccgt
    19501 tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg ccacccaggc
    19561 actgcgtcag gtaggttcca acatcaaacc gttcctctac accgcggcga tggataaagg
    19621 tctgacgctg gcaagtatgt tgaacgatgt gccaatttct cgctgggatg caggtgccgg
    19681 ttctgactgg cagccgaaga actcaccacc gcagtatgcc ggtccaattc gcttacgtca
    19741 ggggctgggt cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga tgggcgtcga
    19801 ctacgccgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg tccacaccga
    19861 atcgctggcg ctgggttcag cgtccttcac tccaatgcag gtggcgcgcg gctacgcggt
    19921 catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg aaaacgatca
    19981 gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgtg atattccggt
    20041 gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg aagatgtcgc
    20101 tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc aggcaaacca
    20161 ggcgttagtt gcgaagactg gcgcgcagga gtatgcaccg cacgtcatca acactccgct
    20221 ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag gctggcaggg
    20281 taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga aaaccgggac
    20341 cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggcgttg tgacctcggt
    20401 ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt ccggagcgat
    20461 taaagatcag atatcaggtt acgaaggcgg tgccaagagc gcccagcctg catgggacgc
    20521 ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc cgccgggtat
    20581 tgtgacggtg aaaatcgatc gcagcaccgg gcagttagct aatggtggca acagccgcga
    20641 agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg tgggaacgac
    20701 cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct tcggcgcctt
    20761 ttcagtttgc tgacaaagtg cacttgttta tgccggatac gggtgaacgc gtcatccggc
    20821 taacaaaatc gtgaaaactc aatatattgc aggaacccca taagcctgat aagcgttgtg
    20881 catcaggcaa acttcacgca tttacactcg cccctgccct ttcaaccatt cgcgcacgag
    20941 gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca aatccatcat
    21001 atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt ccagcgattc
    21061 cgggtagaga tcttgcgcta ccacgatatt cattttgctg gaaaagtaag acggtgccat
    21121 gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat ccaacctctt cttttagctc
    21181 gcggttagcg gcttcgtaga cgctttcacc cggatcaatt aatcctttcg aaaaacctaa
    21241 ttcgtaggat tcagttccca ctgcatattc gcggatcagg atcaggtgat cgtccacaat
    21301 cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa cacgccgcac
    21361 gccattgctg aactccagat ccacgctctc gacggtaaac agtcgggaac gggctacagt
    21421 ttcaacattc agaatggtgg gtttttgtaa tgatttgctc atcgtgggat ctatgctgtg
    21481 aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt gcatgttatt
    21541 tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc aataggttga
    21601 aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga tcagcggtta
    21661 ctgtgatgtg cttaatgatg ctcaagttaa actccacgct tgccgatagc caaccgcaga
    21721 atcatgtatt gtgtccggtg cgactgacca cgcctgacag actaagtaag atggggaaag
    21781 catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac ttgcgggatg
    21841 gctgataaaa gtgcgatcca gacggcgtca gctgccctgg accaacgcct tcgtggatgc
    21901 gcaaacgcgt aaactcacac ctgaagaacg tagcgccgtt gaaaattatc ttgagagcct
    21961 gacgcaggta ttacaggtgc ctggcccaac gggagccagc gcggcaccga tctctctggc
    22021 gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacgc gttacggcat
    22081 ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag tccacctgcc
    22141 ccccttctgg gaacagtaca tcaacgatga gaataccgtt gaactgattc ataccgattc
    22201 gctgccgttg gttatttcac tcaacggtca tacgctgcag gagtacatgc aggaaacgcg
    22261 cggctatgcc ttgcaacctg ttccgtccac gcaggcgtcg attcgcgggg aagagagtga
    22321 gcaaatcgag ctactcaata ttcgcaaaga aacgcatgaa gagtatgcgc tgagtcgtcc
    22381 gcgcgggctg cgtgaagcgt tgctgatcgt cgccaccttt ctgatgttct ttttctgcct
    22441 gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcattactgc tgctgggcgc
    22501 aggtctgtgg gggctattcg cccccccggc aaaatcctcc ctgcgggaaa ttcattgtct
    22561 gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac agatcaacaa
    22621 tatttcactc ggtattatcg acctggtcta tcccgcacac tggcagccat acattgctca
    22681 ggatctcggt caacaaaccg atatcgatat ctatctcgac cgccatgtag tgcgtcaggg
    22741 acgatatctt tcgctgcatg atgaagtaaa aaactttccg ttacagcact ggctgcgcag
    22801 tacgattatc gctgcgggct cgctgctggt gctgtttatg ctgttattct ggatcccgct
    22861 ggatatgccg ctgaaattca ctctctcatg gatgaaaggc gcgcagacca ttgaagccac
    22921 cagcgtaaaa caactggctg acgctggcgt gcgggtaggc gatacattgc gtattagcgg
    22981 tacgggaatg tgtaatattc gcacttccgg aacctggagc gcgaaaacca attcaccttt
    23041 tttaccgttt gactgctcgc agatcatctg gaatgacgcc cgctcattac cattaccaga
    23101 atctgaactg gtcaacaaag cgacggcatt gactgaaaca gttaatcgcc agctgcaccc
    23161 taaaccggaa gatgaatctc gcgtcagcgc ctcattacgt tcagcaattc aaaaatccgg
    23221 catggtattg cttgatgatt ttggcgacat tgtactgaag acagcggatc tgtgttctgc
    23281 caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca gtaaagactg
    23341 ggacgcgctg gtaaaacgcg ccaacgccgg gaagctcgat ggcgtgaatg tgttattacg
    23401 cccggtgagt gcggaatcgc tggataacct tgtggcaacc tccaccgcgc cgtttatcac
    23461 gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg gattcctgat
    23521 tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat cactttacga
    23581 ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc tgatgcatac
    23641 gccgtttaac gccgagggta tcgtcacaaa aatcttcact gacgccaatg gtacgcagca
    23701 tataggcctt catccgatcc cggatcgttc cggcctgtgg cgctatctca gcaccacatt
    23761 gctgctactg acgatgctgg gtagcgccat ttacaatggc gtacaggcct ggcgtcgtta
    23821 ccagcgtcat cgcactcgca tgatggagat tcaggcctat tatgaaagct gcctgaaccc
    23881 gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg caggttatgc
    23941 taccctgtcg cgcaaattgc ttcactctgg agatttccct catgcatatc aacattgcct
    24001 ggcaggacgt agataccgtt ctgctggata tggacggcac gttgctcgac ctcgccttcg
    24061 ataactattt ctggcaaaag ctggtgcctg aaacatgggg cgcgaaaaac ggggtcacgc
    24121 cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat acgctaaact
    24181 ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg atgaccaccg
    24241 agatgggacc gcgtgccgta ctgcgtgaag ataccattcc gtttcttgag gcactgaaag
    24301 ccagcggtaa gcagcgaatt ttgctcacca atgcgcatcc gcacaacctg gcggtaaaac
    24361 ttgagcatac cggtctggac gcacaccttg atttattact ttccacccac acatttggtt
    24421 atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt ctgaaagctg
    24481 aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg caatttggta
    24541 ttcgttactg cctcggcgtg actaatcctg actcagggat tgccgagaaa cagtatcaac
    24601 gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga aggagacgcc
    24661 atgaaagaaa aacctgctgt tgaggttcga ctggataaat ggctatgggc tgcccgtttt
    24721 tataaaaccc gcgcgctggc ccgtgaaatg attgaaggcg gtaaggtgca ttacaacggg
    24781 cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct gcgccaggga
    24841 aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc cgccagcgag
    24901 gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa aatggcgctg
    24961 gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga caaaaaagag
    25021 cgccgcgacc tgttacgatt tacacacggc gacagtgaat aactgttacc tgcaagagag
    25081 atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaattt tgccgttcgc
    25141 ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaacca cgattatccg
    25201 cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt aaccgctacg
    25261 ctgaagtttg atggtgatat caccgtacag ctgcagggcg acggtccgat gaatctggcg
    25321 gttattaacg gtaacaataa ccagcaaatg cgcggtgtgg cgcgcgtgca gggcgaaatt
    25381 ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat caccattacc
    25441 ccgagcgaag gtgaacgcta tcagggcgtg gtgggtctgg aaggtgatac cctggcggcc
    25501 tgcctggaag attactttat gcgttctgaa cagctgccga cgcgcctgtt tattcgcacc
    25561 ggcgacgtag acggcaaacc ggctgcgggc ggtatgttgt tgcaggtaat gcctgcgcaa
    25621 aatgcccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat caaaaccgaa
    25681 gaactgctga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga agaagaggtg
    25741 acggtttacg atccgcagga tgtggagttc aaatgcacct gctcgcgtga acgttgcgcc
    25801 gatgcgctga aaacgctgcc tgatgaagaa gtcgatagca tcctggcgga agatggcgaa
    25861 attgacatgc attgtgatta ctgcggtaac cactatctgt tcaatgcgat ggatattgct
    25921 gaaatccgca acaacgcgtc tccggcagat ccgcaagttc attaatgagt atgtccggca
    25981 gagagggctc tgccggattt tacgactaat acacctgatg cgctttgtca ctcattcaga
    26041 ttcacatcca gcctttcacc atccactaca agatcgcgcc acgctttagg cttcgttccc
    26101 agttttccat ccttcgcagc tgtgagtaat ttttctttcg ttatatcacg cggaagtaaa
    26161 ctcatcgtaa gctcttgcca ttctttcttg tcaggatcaa agccatagac aatgtatctt
    26221 tcatcattaa aagcaaacag gatccgctcc gcctgaccat cactattcag atcttgctcg
    26281 accagtacgc aggcgtcttt ttcaatacag gtcatcacgt tatagcgttc tttgattaat
    26341 gccgaccaga atgccgcatc cggtttgcca gaacctggtg caattaacac attttcggct
    26401 aatgattttt ccgacaccac cttctgaaga ctttgctccc catctaacgc catcagcaga
    26461 tcccacgcgc gtttcgggtc tttcataaac ccggcatcgc ttttcagcga ctcaagcgcc
    26521 gcacgtccat agtgaccgct ctgttcgagc atgtagatag ttacctggcc tggcgtgttt
    26581 ttgccgctct gataacgcgc catatgactg ttcacgctaa tgcgcatact gtccagcacc
    26641 ggagaattaa gaagcaccag tattaccaac accaataacg aaactgcaag gttcactttg
    26701 ccctgaagaa caaggggatt ttgcccttta cgccatagga tactgacaaa ataccccagc
    26761 gaccatacca gtaacaccag caccaccagc gcgccctgca agcggtcgac agtccagccg
    26821 tactgagcga cccgcagcca taacgcccag gcggcgacga acacatacag cggagcaacc
    26881 agcaaagcgg ttttaatcag gcaacgcaac ggccccgtcc agggaagtga cgctttttgc
    26941 ggatcgcgga caatagccat taagatcaat tgcaaaaagg ccagcgttaa cagcaaccca
    27001 gcggcggaga tgtggcgaga aatcgcactc aggcctgtaa acggcagggt gatgataaac
    27061 atcagggtta gcaatgatac taacggcagc aaccccgtgg cgattaacgt gaacaacttt
    27121 tgaatagagt cgattaaacg tgactgtgtt cgcgcgagga tcaccgccag cgcggtaacc
    27181 agacctaatg taagatacat aaaccagtcg gttgcaaaaa agagcgtatt gaaaaacgtg
    27241 atgccaacaa gtttaaacaa ctcaccccac agtaaaagca ccagccacgt caggccattg
    27301 gcgagaaaaa taacctgtaa tataagtacg ttatgccata ctgactggta aaaatagcga
    27361 tagcggctac tgtcattgcg gatacgaagg ctttgttgta tccacggtag caacaacatt
    27421 gccatcagca gcagatagca gccaaaatcc caaagcgcct tttcagctct ccaggggttc
    27481 atgccatcag tttgccactt caaccagccg ctcatcccca gcgtggcaat aaacaccagc
    27541 gccagccatc cccaaaggcg cttctgttta aaagagatca cggagaagag taaaacggat
    27601 gaaaacgcaa cggtcgcagg cacgccataa acaatccaac tgtgattttt tccggcaagc
    27661 caggcgatca acaggtagca aaccagcccc tgaagtaatc cggtagcaat catcccccag
    27721 cgtgttgcgg gtgaaagttc aacgttatcc atgttggtta tccagaatca aaaggtgggt
    27781 taattatcgc atccgggcag tagtattttg cttttttcag aaaataatca aaaaaagtta
    27841 gcgtggtgaa tcgatacttt accgattgaa tttgcatcaa tttcattcag gaatgtgatt
    27901 ccactcacaa tattcccgcc atataaacca agatttaacc ttttgagaac attttccaca
    27961 cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg acaggaatca cggagttttt
    28021 tgtcaaatat gaatttctcc agatacgtaa atctatgagc cttgtcgcgg ttaacacccc
    28081 caaaaagact ttactattca ggcaatacat attggctaag gagcagtgaa atgcgcgtta
    28141 acaatggttt gaccccgcaa gaactcgagg cttatggtat cagtgacgta catgatatcg
    28201 tttacaaccc aagctacgac ctgctgtatc aggaagagct cgatccgagc ctgacaggtt
    28261 atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt cgataccggg atcttcaccg
    28321 gtcgttcacc aaaagataag tatatcgtcc gtgacgatac cactcgcgat actttctggt
    28381 gggcagacaa aggcaaaggt aagaacgata acaaacctct ctctccggaa acctggcagc
    28441 atctgaaagg cctggtgacc aaacagcttt ccggcaaacg tctgttcgtt gtcgacgcct
    28501 tctgtggtgc gaacccggat actcgtcttt ccgtccgttt catcaccgaa gtggcctggc
    28561 aggcgcattt tgtcaaaaac atgtttattc gcccgagcga tgaagagctg gcaggtttca
    28621 aaccagactt tatcgttatg aacggcgcga agtgcactaa cccgcagtgg aaagaacagg
    28681 gtctgaattc cgaaaacttc gtggcgttta acctgaccga gcgcatgcag ctgattggcg
    28741 gcacctggta tggcggcgaa atgaagaaag ggatgttctc gatgatgaac tacctgctgc
    28801 cgctgaaagg tatcgcttct atgcactgct ccgccaacgt tggcgagaaa ggcgatgttg
    28861 cggtgttctt cggcctttcc ggcaccggta aaaccaccct ttccaccgac ccgaaacgtc
    28921 gcctgattgg cgatgacgaa cacggctggg acgatgacgg cgtgtttaac ttcgaaggcg
    28981 gctgctacgc aaaaactatc aagctgtcga aagaagcgga acctgaaatc tacaacgcta
    29041 tccgtcgtga tgcgttgctg gaaaacgtca ccgtgcgtga agatggcact atcgactttg
    29101 atgatggttc aaaaaccgag aacacccgcg tttcttatcc gatctatcac atcgataaca
    29161 tcgttaatcc ggtttccaaa gcgggtcacg caacgaaagt tatcttcctg accgcagacg
    29221 cctttgctgt gctgccgccg gtttctcgtc tgactgccga tcaaacccag taccacttcc
    29281 tctctggctt caccgccaaa ctggccggta ctgagcgtgg catcaccgaa ccgacgccaa
    29341 ccttctccgc ttgcttcggc gcggcattcc tgtcgctgca cccgactcag tacgcagaag
    29401 tgctggtgaa acgtatgcag gcggcgggcg cgcaggctta tctggttaac actggctgga
    29461 acggcactgg caaacgtatc tcgattaaag atacccgcgc cattatcgac gccatcctca
    29521 acggttcgct ggataatgca gaaaccttca ctctgccgat gtttaacctg gcgatcccaa
    29581 ccgaactgcc gggcgtagac acgaagattc tcgatccgcg taacacctac gcttccccgg
    29641 aacagtggca ggaaaaagcc gaaaccctgg cgaaactgtt tatcgacaac tttgataaat
    29701 acaccgacac ccctgcgggt gccgcgctgg tagcggctgg tccgaaactg taatgatttg
    29761 aagctggaga atatctatcc agtatcttat agaaagcaaa acgggaggca ccttcgcctc
    29821 ccgtttattt acccttcttt tgtcgtgccc tgcgcccgcg ttaccggcac aggcagccag
    29881 gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc gttatggtta
    29941 tccacgatac gctgcacaat cgccagccct aatcccgtgc cgctaatggt gcgtgcactg
    30001 tcgccgcgaa caaacggctg gaaaaggtgc ttacgttgtt ccggcgcaat tcccggaccg
    30061 tcatcttcca cctggaacca ggcgcgattc ggctccgtcc cgctgctgac tttgatccag
    30121 ccattgccgt aacgggcggc gttgaccacc atattcgcca ccgcgcgttt gatcgacagc
    30181 gggtgcattt tcacctcaat gctgccgggg taaagcgcgg tttcaatttc ccgctcatag
    30241 ccactttcgg cagcaataac ctcaccgagc acggcgttga gatccgccat ttccatcggc
    30301 atctcctgcc cggtgcgcag gtagtcgata aactgctcaa tgatggcgtt gcactcctcg
    30361 atatctttat tgatcgattc tgccaggtag ccatcctgct cgctcatcat ctcggtcgcc
    30421 aggcgaatac gcgtcagcgg cgtgcgcaga tcatggctta cccccgccat cagcagcgtg
    30481 cggtcatccg ccagttgctt aacaccagcc gccatatggt taaaggcgcg ggtaaccgaa
    30541 cgtacctcgg acgcgccata ttcacgcagc ggcggcggaa taatcccttt accgacctgc
    30601 aaagcagcat gttcgagatc gaccaacggt cgattctgga tacgaataaa cagccacgcc
    30661 ccgcctatcg ccaatagcat aatcgccaac gtatagcgga acagcggaga gaaatcgccc
    30721 tgatgaattt cggtcagcgg cacgcgtacc cagatattgg gcgacagcca ggttttcagc
    30781 cagacgacag gcgaactttt gttgacctca acgcgcactt ccgtcgggcc gcccagttgc
    30841 tgtgccatct gatggcttaa gaattcatag tgttgtgccc aacgcagacc tgcctcttcg
    30901 gcagcttcgt tggagtagag agagatcccc agctcacggt agatctcccg acggaaagcg
    30961 ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat caacatacgc
    31021 acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc gaagttcagc
    31081 accaccagat aagtcgtcac caggctggcg aacagcaagg tgacgatgag caataacgta
    31141 cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagag ccgtccggta
    31201 caaagacgta gcccaggccc caaacggtct gaatgtaacg cggatgcgct ggatcttctt
    31261 ctaccatgcg gcgcagacgc gaaatctgca cgtcgatgga gcgttccatt gcggaatatt
    31321 cacgaccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc ggatggctga
    31381 ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg catcggctcg tcttcgcgga
    31441 acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc gcctcttcct
    31501 gtgacggtgc gcctggtagc tcgttcgcct gacgacgcag caccgcacgg atacgggcca
    31561 gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc agcgccaatc tccaggccaa
    31621 cgatacggtc cacttcttcc cctttcgccg tcaccataat gatcggcatc gggttgctct
    31681 gactacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatc aaatccagta
    31741 ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca ttagcgacgc
    31801 ttcgaacctg gaagccttgt tcggtgagat aacgctccag cagcgcacgc aggcgcatgt
    31861 cgtcatcgac caccagaatc ttgtagttct cttgcattgt ttgtactccc aaaggttcgc
    31921 aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct aaatctggta
    31981 tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta tacaatttat
    32041 tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt gcgcattatc
    32101 aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga agggtatgaa
    32161 aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag
    32221 gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca gtataataaa
    32281 aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat ggaaaatctc
    32341 aaaatcgtcg attactcccc tcagcaggaa ggcaaagtct tttttggcgc gtgggtggag
    32401 attgaaaacg acgatggcgt gactcaccgt ttccgtatag tcggttacga tgaaattttt
    32461 ggccgtaaag attacatctc tatcgattcc ccgatggctc gcgcattgct gaaaaaagag
    32521 gtgggcgatt tggcggtggt gaatacccct gccggggaag cgagctggta tgttaatgct
    32581 atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct ggcatttttg
    32641 ccagccagtc cgtataacta tcccctgatt tttgatccga taagatgaac tcaaaccatg
    32701 atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt
    32761 gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc acgttatcgt
    32821 aaggaaatca ccggcggtct ggatgacacg cagttgcgta atctggaaac gcgtctggga
    32881 tacctgcgtg aactggaaga gagacgtcag gcgatcctca aatccatttc cgagcaaggc
    32941 aaactcaccg atgatctggc gaatgccatc aacgccaccc taagcaaaac cgaactcgaa
    33001 gacctctacc tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca
    33061 gggcttgagc cgttggctaa cctgctgtgg agcgatccgt cacacacgcc agaagtcgcc
    33121 gctgcgcaat atattgatgc cgataaaggc gtggcagata ccaaagccgc gctggacggc
    33181 gcgcgctata tcctgatgga acggtttgcc gaagatgccg cgctgctggc gaaagtgcgt
    33241 gattatctgt ggaagaacgc gcatttggtt tctacggtgg tgagcggtaa agaagaggaa
    33301 ggggcgaaat tccgcgacta tttcgatcat cacgaaccgt tgtccacggt gccttctcac
    33361 cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac ttcagctttc gctgaatgcc
    33421 gatccacagt tcgatgagcc gccgaaagag agctattgcg agcaaatcat catggatcac
    33481 cttggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt ggtgagctgg
    33541 acgtggcgca tcaaggtgct gatgcatctg gaaaccgaac tgatgggcac cgtgcgcgaa
    33601 cgtgcggaag atgaagcaat caacgtcttt gcccgtaacc tgcacgatct gctgatggcg
    33661 gctccagccg gactgcgtgc cacgatgggc ctcgatccgg gtctgcgtac tggggtaaaa
    33721 gtggcggtag tcgatgcaac cggcaaactg gtggcgaccg ataccattta cccgcacacc
    33781 ggacaagccg caaaagcagc gatgactgtt gctgcgctgt gtgaaaagca taacgttgaa
    33841 ctggtggcga tcggcaacgg tacagcttcc cgcgaaaccg aacgtttcta tctcgacgtg
    33901 cagaagcagt tcccgaaagt gaccgcacag aaagtgatcg tcagcgaagc tggcgcgtcg
    33961 gtttactcgg cttccgagct ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt
    34021 ggcgcggttt ctatcgcccg ccgtttgcag gatccgctgg cggagctggt gaaaatcgat
    34081 ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca actggcccgc
    34141 aaactggatg cagtagtaga agactgcgta aacgccgttg gcgtcgatct caacaccgct
    34201 tctgttccgc tattaactcg cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg
    34261 gcctggcgcg atgagaacgg ccagttccag aaccgtcagc aactgctgaa agtcagccgt
    34321 ctggggccga aagccttcga gcagtgcgcg ggcttcctgc gcattaacca cggtgataac
    34381 ccgctggacg cctctaccgt tcacccggaa gcctatccgg tggtggaacg cattctggca
    34441 gcaacacagc aggcactgaa agagctgatg ggtaacagca gcgaactgcg taacctgaaa
    34501 gcgtctgact ttactgatga aaaattcggt gtgccgacag taactgacat catcaaagag
    34561 ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc
    34621 gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggcgctgt caccaacgtc
    34681 accaactttg gcgcgtttgt cgatatcggc gtgcatcagg acggcctggt tcacatctct
    34741 tcattgtcga acaagtttgt ggaagatccg cataccgtgg tgaaagcggg cgacattgtg
    34801 aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac tatgcgtctg
    34861 gacgagcagc ctggcgaaac caactctcgt cgcggcggcg gtaacgatcg cccgcaaaac
    34921 aaccgcccgg cagccaaacc acgcggtcgt gaagcgcagc ctgccggtaa cagcgcgatg
    34981 atggacgcgc tggcggcagc gatgggtaaa aaacgttaaa cgcccggact ggcccctaaa
    35041 cctgcgcgat aaggcgttca cgccgcatct ggcaacgaac gccttgcctg acataaaagt
    35101 gccggaaaat atctccggca tatttattcc acagccaaac tcacaatata ctccgttaat
    35161 atttatcact tcattaacaa ctgaaacctt aattaaacat tagccagtcc tggtaattca
    35221 ctattcgaat tatattttcg ctgcgatata accttgagcc acatcaacat tgagtcagat
    35281 tattattcaa accaacattc gcacacattt taagtattgc tgatagaaac cattctcatt
    35341 atcattgtac tgttgattat ttaatctctc cttcgttggc aaatcatctg gtctcatgtc
    35401 gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag gcacctatgc
    35461 aatacactcc agatactgcg tggaaaatca ctggcttttc ccgtgaaatc agcccggcat
    35521 atcgccaaaa actgctttct ctaggcatgt tacctggctc ctcttttaat gtggtgcgcg
    35581 tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg gtattacgca
    35641 aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt gataacaaca
    35701 atgaaaaaat taaccatagg cttaattggt aatccaaatt ctggcaagac aaccttattt
    35761 aaccagctca ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt
    35821 aaagaagggc aattctccac caccgatcat caggtcacgc tggtggatct gcccggcacc
    35881 tattctctga ccactatctc atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac
    35941 attttgagtg gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt
    36001 aacctgtacc tgacgctaca actgctggaa ctcggcattc cctgcatcgt ggcgctgaac
    36061 atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct gtcggcgcgt
    36121 cttggctgcc cggtgatccc gctggtttca acccgtggtc gcggtattga agcgctcaag
    36181 ctggcgattg atcgctataa agctaacgaa aatgtggaac tggtgcatta cgctcagccg
    36241 ctgctcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa
    36301 cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc ctacgccggt
    36361 gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc gtaatgagat ggacgatccg
    36421 gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg ccatctgtga tgtggtaagc
    36481 aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat cgtgctcaac
    36541 cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttcct gctggctatc
    36601 aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtggcgct atttgtgcat
    36661 ggtattcaat ggattggcta cacgctccac ttcccggact ggctgactat cttcctcgcc
    36721 cagggcctgg gtggtggtat taacaccgtg ctgccactgg tgccgcagat cggcatgatg
    36781 tacctgttcc tctccttcct tgaggactcc ggctatatgg cgcgtgcggc gtttgtgatg
    36841 gaccgcctga tgcaggcgct gggcttgccg gggaaatcct ttgtgccgct gatcgtcggt
    36901 ttcggttgta acgtaccgtc ggtaatgggc gcacgtacgc ttgatgcacc gcgtgaacgt
    36961 ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc tatcttcgcg
    37021 gtattcgcgg ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc gctgtatatg
    37081 ctgggtatcg taatggcggt gctgactggc ctgatgctca agtacaccat catgcgcggt
    37141 gaagcgacgc cgtttgtcat ggagctgccg gtctaccatg taccacacgt taaaagcctg
    37201 attatccaga cctggcagcg cctgaaaggc ttcgttctgc gtgctggtaa agtgattatc
    37261 atcgtcagca ttttcctgag cgctttcaac agcttctcgt tgagcgggaa aatcgtcgat
    37321 aacatcaacg actcggcgct ggcttccgtc agccgggtga tcaccccggt cttcaagccg
    37381 attggcgtgc atgaagataa ctggcaggca acagttggcc tgtttacggg tgcaatggcg
    37441 aaagaagtgg tggtgggtac actcaacacc ctctacaccg cagaaaatat tcaggacgaa
    37501 gagttcaatc cggcggaatt taacctcggt gaagagttgt tcagcgcggt agatgaaacc
    37561 tggcagagcc tgaaagacac cttcagcctt agcgtactaa tgaaccccat tgaagctagc
    37621 aaaggcgacg gcgaaatggg taccggggcg atgggtgtga tggatcagaa attcggtagc
    37681 gcagctgccg catacagcta cctgattttc gtcctgctgt atgtaccgtg catctcggtg
    37741 atgggagcca tcgcccgtga atcaagccgt ggctggatgg gcttctccat cctgtggggg
    37801 ctgaatatcg cttactcact ggcaacattg ttctatcagg tcgccagcta cagtcagcac
    37861 ccaacttaca gcctggtgtg cattctggcg gtaatcctgt ttaacttcgt agttatcggt
    37921 ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg caagtcggta
    37981 agtagttgct gcgcagccag caccaccggt gattgccatt aatggcttca cttattcagg
    38041 tacgtgattt gctggcgtta cggggccgta tggaagcggc ccagataagc cagacattga
    38101 acactccaca gccaatgatt aacgccatgc tgcaacaact ggaaagtatg ggcaaagccg
    38161 tgcggattca ggaagaacct gacggctgcc tctccggcag ttgtaaaagc tgtccggaag
    38221 gaaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc atactccatc gcctgttttg
    38281 aaaagcccgg tatgcgtttg catccgggct tttttgcgtg cggctttcca taaaaatgca
    38341 attcttgcag ctcgacgtaa gttcctctga aagcgtctcg cagggatgaa aactggctaa
    38401 tgcacaggtg tggagtggcg cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc
    38461 atgagcaaaa agcagagttc caccccacac gatgcgctgt tcaaactctt tttacgccaa
    38521 ccggagacgg cacgcgattt tcttgcgttt catttaccgg cacccattca cgcgctctgt
    38581 gatatgaaaa ccctcaagct ggagtcgagc agctttattg atgacgatct gcgtgaaagc
    38641 tattccgacg tgctgtggtc ggtgaaaacg gaacaaggac caggatacat ctattgtctg
    38701 attgaacatc aaagcacctc aaacaaattg atcgcattcc gcatgatgcg ttacgccatt
    38761 gccgcaatgc aaaatcacct tgatgccgga tacaaaacgt tgccgatggt ggtgccattg
    38821 ttgttttatc acggtattga aagcccctat ccctattcgc tgtgttggct ggattgtttc
    38881 gccgatccca aactggcaag gcagctttat gcctccgcat ttccgctgat tgatgtcacc
    38941 gtcatgcctg atgatgaaat catgcagcac cgacgtatgg cgctgctgga gttaattcaa
    39001 aaacatattc gtcaacgcga cctgatgggg ctggtagagc aaatggcctg cttattaagt
    39061 agtggatacg ctaatgacag acaaatcaaa gggctgttta attacatctt gcaaaccggc
    39121 gatgcggtac gttttaacga ttttatcgac ggcgttgccg aacgttcacc gaaacacaag
    39181 gagagtttaa tgactattgc ggaaagattg cggcaggagg ggtttcagaa aggtatacgt
    39241 actattgcca aaaccatgct ggatgccgga gtccctcttg aggacgttct gcgatttacc
    39301 gggctgtcag aggaagaatt ggctgcggcg agccagtaga cactatcccc tgcttaagtg
    39361 ctactttgtg ctatcttgca tgtaacacaa agtagcactt aggaactgat atgcagacag
    39421 tgaaattacg gcaacaagga ggagccatga tcgttactat cccacgagat cttgccaccg
    39481 atcttggctg gtcaccgggc acagagctta ccgtggagaa aaagggcgat agcgttaatt
    39541 tgcgcgccac agagcacaaa cctcgtgggc gtcttaccgt cgcgcagttg ttgagccaga
    39601 tcgatgaaaa cgaaattaag gccttaaatc agtcctcaga aggatgggct gaagagaaaa
    39661 aaggtaatga agcatggtaa aggcacggac gccacatcgt ggtgagatct ggtgttttaa
    39721 ccctgatccg gttgccgggc atgaacttcg gggtccacat tattgcattg tggtaacgga
    39781 caaagagctc aacaatgttt taaaagttgc tatgtgctgc ccgatttcaa caggggcaaa
    39841 tgcggcacgt tcctcagggg tgacggtgaa cgtcctcccc catgatacgc aaaccggtaa
    39901 cctgcatggc gttgtgctct gtcaccagct aaaagctgtc gatcttattg cccgtggcgc
    39961 taaatttcat accgttgccg atgaagcatt gattagtgag gtgatcggta aactggtgaa
    40021 tttaatcgac ccacaacagt agccctgact taccacctac accctctgct tcaacgccac
    40081 cagcaggtga caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa
    40141 gatatacgac tcgctgtgtg gccagagttt atccagcatc ggcaccactt ttcgcggcac
    40201 cagaccatcg agatagccat acagtcgcaa aaacggcatg ggtacgtttt gcagcggctg
    40261 gcggagatcg accgttttca gaatttccag cccaccattg agcacgtcaa cctccggcat
    40321 cggtaacgcc agaacggttt tcttcaacgc ccgcgcatcc tgacgcgccg tttcagtacc
    40381 catggtttgt aacgccagga accgttccac cgtgcgctga aaatcatcac tgagttgctg
    40441 ctgaaatccc gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca
    40501 aggtgacgac gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg
    40561 gcttgccacc agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgtcg
    40621 cagcacggct tcggccatat cagcaagtga catcgcacca aatccccggc tacgcccgaa
    40681 gccgggcagg tcaacaaggt gcagcgtaaa atgcgagctc agttcctcgt caatgcaacg
    40741 ccacacttcg gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc
    40801 tttggtctgc caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga
    40861 acatatgcta acagtaccgg gattatgctg gctatgccga atgccgctgg cgttaggtca
    40921 ttgggggatt tgttcggtct gctcacgcgc cgcccgtaca gataaaacgt tatgcccaca
    40981 atgtggatta cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccacc
    41041 gccctggcaa agactggtca cggttgccga ctatgcgccg ccgttaagcc cgctaatcca
    41101 ccagcttaag ttttcccggc gcagcgaaat cgccagcgcc ctgtcacgtc tgttactatt
    41161 ggaggtctta cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt
    41221 tccgttatgg cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca
    41281 gccgttatca cgctggttgc actgccgatg ggatagcgaa gccgtcacac gaacacgggc
    41341 cactgcgacc cagcattttc tcagtgcccg gctacgcaag cgcaacctga aaaatgcctt
    41401 tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac
    41461 cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca
    41521 ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta
    41581 aaatagtcaa ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact
    41641 ttgccaaact gctggcaaac caggaagaag ggacacaaat ccgcgtattt gtgattaacc
    41701 ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca
    41761 ccgacactgc cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat
    41821 acctggaaga tgcagagatc gattttgtta ccgaccagtt gggctctcag ctaacactga
    41881 aagcgcctaa cgccaaaatg cgtaaagtgg cagacgatgc accactgatg gagcgcgtgg
    41941 agtatatgct gcagtcgcag atcaacccac agcttgctgg tcacggtggc cgcgtttcac
    42001 tgatggaaat caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt
    42061 gttccatggt cgatgtgacg ctgaaagaag ggatcgagaa gcagctgcta aacgaattcc
    42121 cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact
    42181 aagttatccc ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac
    42241 gttgaacatg tgccagacgt tggcgcagca ttgctttggc aaatccctcc cttcgtttcc
    42301 ccgcgataat atgaccaacc tctcataatt taaatttacc ccgctctagt gattctcaaa
    42361 cgccagatgt tacccgtatc attcacatgg gtaccaaaca tactcctgac atctgactac
    42421 aataattagt tttagtgggt atcagtcatg gtgccgcaat atctctgttc ccgattggga
    42481 taattagagt ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa
    42541 tcatgccatt agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgct
    42601 tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag
    42661 gaatgccgct ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta
    42721 gccttgccct gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg
    42781 gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaaacac atccagtggg
    42841 cggtggtact gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc
    42901 tgatgctgcc gctggtgttt accatcgcgg cttccgcgaa tattccactg ctgtatgttg
    42961 gtgtaccaat ggcagctgca ctgtctgtga cccacggctt cctgccaccg catccgggcc
    43021 cgactgcgat tgccaccatt ttcaatgccg acatgggtaa aaccctgctg tacggtacta
    43081 ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta
    43141 tcgataagcc aattccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc
    43201 cgagctttgg cgtcagcgtc tggacctctc tggtgccggt agtactgatg gcgatgcgtg
    43261 cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg
    43321 gtgacccggt aatggctacg ctgattgccg tgctgattgc gatgttcacc tttggtctaa
    43381 accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg
    43441 cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg
    43501 gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg
    43561 cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca
    43621 ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagcccg gagctgatgg
    43681 ttattgcggt tggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc
    43741 tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg
    43801 aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat
    43861 aacactgact gccggatgca tcgcatccgg catcaaccgc actctacttc ttcttcgctg
    43921 cagctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacgccatca tcggcaaaca
    43981 tcgtctcaag cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag
    44041 gaatattcac cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac
    44101 tgttactgtc ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc atcgacatca
    44161 acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca
    44221 gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat
    44281 acagccccag ggttttgccc agagttagat ccccgctctc ccagtagccg cgtagcgtag
    44341 gcaggtcatg tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga
    44401 acgtcttctc gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc
    44461 gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc
    44521 gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat
    44581 acgcgccctg atccgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca
    44641 catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg
    44701 gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc
    44761 ccaacgggcc gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tatagttcac
    44821 ggtcacacca ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc
    44881 caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca
    44941 gccactgcag ccagagataa aaatcaacgt catcgcgatg ttcttcgcag aactgacgca
    45001 cttctggtga atccacgttc tgatacatct ctggccatgc aggccagccc cagcgcattt
    45061 cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt
    45121 cgccttgatc tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga
    45181 aacctttcca cgccattcgt aatactgtca tttttagggc ggtaaccgtg gagtaatcga
    45241 cccagtcggc atcccgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg
    45301 cctgagcctc ttcgctaaga tggaaatctt cgacggcgtt aacgtcgata taaatcacat
    45361 tcagccaacg gcgagaagac gggctgtatg ggctggcact ctccggattt gccggataga
    45421 gcgcatgaat cgggttcagg ccgatgaacg acccgccacg ttttgccaca tccaccagca
    45481 tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa
    45541 gctggacgca ggcaccccac agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc
    45601 gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgtcagcg
    45661 tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc
    45721 ctttgtactg cgttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca
    45781 ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg
    45841 ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc
    45901 taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg
    45961 cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac
    46021 gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga
    46081 ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg
    46141 cctctacata ggctgcgaaa tccgccatca ccagatacgg atcgccgccc tgtttgccga
    46201 tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtatttg ccgctttcca
    46261 gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc
    46321 ctttggccag aattgccttc acttgttcca cggtatgacc aaaaataaag atattttctt
    46381 caccgacttt ctcggcgatt tcaacgttcg ccccatccag cgtaccgaca gtaagcgcac
    46441 cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt
    46501 gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca
    46561 ggaacaccac cttcaactta tcgccaacca gcggatcgtt gttgatcacg tcagccactt
    46621 tgttgatcgc aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga
    46681 agaggaagac gcgcggtacg cgatcagcct gcgggttttc acgaatttct ttgtacaacg
    46741 ccagaatatg cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa
    46801 tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca
    46861 gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag
    46921 cgaatttttc cagattgatc agctgatcga gatcgttagc ccactctttt ttcagtgatt
    46981 tatccaacag agccgccagt gccgggttgc actgtttgat ccagcgacgt ggggtaatac
    47041 cgttggtgac gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt
    47101 tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac
    47161 acaggttcgc catatgcact tgtttgtcgt gcaccaccgc cagtttggcc cacacttttt
    47221 catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa
    47281 tctgcatgtg gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt
    47341 ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat
    47401 cccagctcat ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgatagttg
    47461 ggtgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg
    47521 ccagatgatg gcgacgcaaa atatccgcta ccgaacaggc acactggaag tattgctgca
    47581 tcaggcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt
    47641 tttccgcatt gatgccctgc tgttcggcac gcaggaaatc accgtcgtta aatttagtca
    47701 gatcaaacgg atgcgcatgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac
    47761 gataaccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc
    47821 gtccgtcttt cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc
    47881 ggaaccacgg gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcga
    47941 caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac
    48001 cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca
    48061 gcgccgggtc gatctcttct tccagcaggt cagtcagatt gatgtcataa gccttcaacg
    48121 aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc agacgaccaa
    48181 tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccaccggt ttggcgaatg
    48241 gctgagcacg cagcatttcg gccagtgctt cactcactgc cagccaccac tggcgaggag
    48301 tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa
    48361 attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac
    48421 acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta
    48481 ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcaaccactt aaagctagtg
    48541 caaaccacag gattagcagc aaatcaatgc catacagcgc agaaaatctg tatctaagtg
    48601 caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag
    48661 gagtgaaaca gtttcacaag taaaatatcc agtgtgctcc atctcattct taatagattt
    48721 attaagatca tctttttaga tagcactttc atcaggaatg aagaagaaac ccttgcttaa
    48781 atgaatctga tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg
    48841 agttgttaat aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt
    48901 catcgcttgc attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa
    48961 aaaaataata tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa
    49021 ttaagtcgtc cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt
    49081 tccggcgcga acaacttccg gctggcgctg atcacaagtc ctgcgggcta cggtaagacc
    49141 acgctcattt cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggacgaa
    49201 ggtgataacc agcaagagcg tttcgccagc tatctcattg ccgccgtgca acaggcaacc
    49261 aacggtcact gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg
    49321 tcactcttcg cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc
    49381 atcgatgact atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt
    49441 cgccatcaac cagaaaatct caccctggtg gtgttgtcac gtaaccttcc gcaactgggc
    49501 attgccaatc tgcgtgttcg tgatcaactg ctggaaattg gcagtcagca actggcattt
    49561 acccatcagg aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca
    49621 gaaagcagtc ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc
    49681 ctctccgccc ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc
    49741 aatgccagcc atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca
    49801 acgcgccatt ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc actcatcacc
    49861 cgtgtgaccg gcgaagaaaa cgggcaaatg cgcctcgaag agattgagcg tcaggggctg
    49921 tttttacagc ggatggatga taccggcgag tggttctgct atcacccgct gtttggtaac
    49981 ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc
    50041 gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg
    50101 gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tctgttcaac
    50161 catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagcttgctg
    50221 gaaaatccgc agttggtgtt attgcaggcg tggctgatgc aaagccaaca tcgctacggc
    50281 gaagttaaca ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc
    50341 atgcacgcag aatttaacgc tctgcgcgcc caggtggcga ttaatgatgg taatccggat
    50401 gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc
    50461 cgcattgtgg caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc
    50521 tcactggcgc taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac
    50581 gccttatgga gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc
    50641 gcgtgggaaa cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagttg
    50701 ccgatgcatg agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg
    50761 gatgaagccg aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag
    50821 caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat
    50881 aacgcccgta gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc
    50941 gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa
    51001 gccgccgctg ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc
    51061 ctgcaaggtc aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct
    51121 gcggaaattg ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat
    51181 ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac
    51241 gcccagcgtg tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat
    51301 tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg
    51361 ctgccggaac tggaacagca tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat
    51421 cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa
    51481 gtgccagaac tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg
    51541 atttactccg gttacagcaa cgaacaaata gcgggtgaac tggaagtggc ggcaaccacc
    51601 atcaagacgc atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc
    51661 gtgcagcacg cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttgtatagcg
    51721 tttttatgta cactctttat actgtatata aaatcgcact acaggctcat gaatatgaaa
    51781 ccggaaagca aagaagcgcc aatcaatatc agagcgaaag cctcacagcg tgacctgatt
    51841 gatatggcag cgaatctggt cgcaaaatcg cgcactgatt ttatgctgga tgccgcatgc
    51901 cgtgaagcgc aagacatctt attagatcaa cgactattca tccttgatga cgagcaatac
    51961 gatgcgtttc tcgctgccct ggacgccccc atcactgctg agcgtcaggc caaaattaac
    52021 gcgttgatga atcgtaaatc gccatgggaa taacggctcc tactccccta acgtctgaac
    52081 ataatctggc tgacttttgc tgttccgatc acgggatgaa tgaatggcta aaaaagaaag
    52141 cgttaaaaaa tcacagcacc ggtctgtctc gtgtttacgt tatctgtatt gtcaataccc
    52201 gccaggtaat cggctattac tgcctttcta caggcagtat tcaacgtaat ttggcccctg
    52261 gagccatgcg acgtaatgct cccgaatcgt tgcctgttgt cgtattaggc agactggcaa
    52321 ttgatcaagc ctgggcaggt aaagggttgg gcgtcgcatt gttgaaagat gcagtctatc
    52381 gcacaatgtc catagctcaa caggtagggg ttcgtgcgct cattgttcat gccttagacg
    52441 attcagtacg caatttctat ctgaagtatg cctttgtacc ttcaccattc cagtcactta
    52501 ccttactgta tcccattact ctggagtaat ttacctctac cggatagtca cactgatgag
    52561 tctgatccgg gagggatttt aatcagtgag tttggttatc atcacccgcg ttacgccatc
    52621 tgtttcagcc agagtaaacc gcacctgcaa gaaacgctcc accacggcga tattggtcag
    52681 cagatggcat gagggatggg cgaccgtaaa ttgtcccgcg cccgccagcg ccattggcaa
    52741 cactagctgg tcagcaagat attcccccac tgccgccggg cttgccaggt agcgtttcac
    52801 ctctttcacc aactgtgccg caaccacctc ggcactgacg cgcttttcac cgacgacaaa
    52861 aaagcgttcg gtgatatttt cactttcgac ttcaagcgag acggtattac ccggcccctg
    52921 gtcgcgcggc aggttatgaa tatactgctc atgcagggga aaactccccg ccagcgtagc
    52981 gatttcacgc tcagcaacat ggcgcggcac accagccagt agcacctctc cacgcatctg
    53041 cacaatgttc ccgcgctcgc caacttgcaa ggttttaaac gatgccactg gcgagacttc
    53101 tgttgccacc acgccgcctc cggcagggta aaaaccgtga cgtaacagcg tggtttgctg
    53161 atgaattcct attttcgcca gcagcggctc caacacccgg cggataaaat ccgcaggcgg
    53221 ggccgacggg ttatcggtac cgccgctcac ttcaacacgc gatggcccgt cggcaaacca
    53281 cagcgcgggc agcaccgttt gcagcaccag cgtacagctt ccggcgctac cgatagcaaa
    53341 gcggtaatcg ccgccgcgca cggtgcccgg ccggaagagc agacgctgcg accccagctc
    53401 cgcgccttcc accgttgccc tacaaatttc cgcagctgct tttaccgcgg tcagatgctg
    53461 gcgcaacagt cccggttttg cccgcccggc acgaatgccg gtgatggtaa atggctggcc
    53521 ggttatcatc gacagactca gcgccgagcg caggatctgc ccgccacctt cgccctgtgc
    53581 gccatccagc gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag
    53641 ggtatagata acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata
    53701 cgccatcggg atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtagc
    53761 acgaatttga tcttccacgc tgaacagttt tttcgcttta gtacggctca ttacccgccc
    53821 ggcaccgtgg ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct
    53881 tttcgcaccc atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc
    53941 tttacgcgtc acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg
    54001 acagttgatc tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct
    54061 ctgcaacgcc gttaccacgt tttccatcat cgcatcgcgg ttaaggctgg caaaaagctg
    54121 cgcccaggcc acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc
    54181 cagatcacgc gacggcaacg tctcaagttg gtcctgcatc tctttttgcg ccagatcgat
    54241 aaagtaagtc ccgatggcat taccaattcc gcgtgaaccg gagtgcagca taatccacac
    54301 ctggtccgac ccatcaaggc agatttcaat aaagtggtta cccgttccca gcgttcccag
    54361 gtgtttatag ttgttagtgt taaggaaacg cggatatttt tgcgttaacc actgataacc
    54421 ggcctccagc tcagcccatt tcgcatcgac gttaacaggc gggttttccc atgcaccttt
    54481 atcacgttta caacgtccgg tagtacgccc gtgcggcacc gcagtttcaa tcgcctgacg
    54541 cagctccgcc aggttttccg gcaggtcagc cgccgttaac gcggtacgca gcgcgttcat
    54601 tccacagcca atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac
    54661 gctaccaatg gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa
    54721 aataaacggc atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac
    54781 gcctttggtc cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt
    54841 gtttttctct tt
//
LOCUS       DAZUGK010000007        54602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZUGK010000007 DAZUGK010000000
VERSION     DAZUGK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.007"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..181)
                     /locus_tag="ACHY5W_000354"
     CDS             complement(<1..181)
                     /locus_tag="ACHY5W_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS4445321.1"
                     /translation="MELSCPICQNPLERNGEATHCATCNKDFIVQALCPDCHQPLQVL
                     KACGAVDYFCQHGHGL"
     gene            complement(244..1716)
                     /gene="der"
                     /locus_tag="ACHY5W_000355"
     CDS             complement(244..1716)
                     /gene="der"
                     /locus_tag="ACHY5W_000355"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4445322.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQSIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     TPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1834..3012)
                     /gene="bamB"
                     /locus_tag="ACHY5W_000356"
     CDS             complement(1834..3012)
                     /gene="bamB"
                     /locus_tag="ACHY5W_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFS4445323.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3023..3643)
                     /gene="yfgM"
                     /locus_tag="ACHY5W_000357"
     CDS             complement(3023..3643)
                     /gene="yfgM"
                     /locus_tag="ACHY5W_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFS4445324.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3661..4935)
                     /gene="hisS"
                     /locus_tag="ACHY5W_000358"
     CDS             complement(3661..4935)
                     /gene="hisS"
                     /locus_tag="ACHY5W_000358"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4445325.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5046..6164)
                     /gene="ispG"
                     /locus_tag="ACHY5W_000359"
                     /gene_synonym="gcpE"
     CDS             complement(5046..6164)
                     /gene="ispG"
                     /locus_tag="ACHY5W_000359"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFS4445326.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6191..7204)
                     /gene="rodZ"
                     /locus_tag="ACHY5W_000360"
     CDS             complement(6191..7204)
                     /gene="rodZ"
                     /locus_tag="ACHY5W_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFS4445327.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(7489..8643)
                     /gene="rlmN"
                     /locus_tag="ACHY5W_000361"
     CDS             complement(7489..8643)
                     /gene="rlmN"
                     /locus_tag="ACHY5W_000361"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFS4445328.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8793..9224)
                     /gene="ndk"
                     /locus_tag="ACHY5W_000362"
     CDS             complement(8793..9224)
                     /gene="ndk"
                     /locus_tag="ACHY5W_000362"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4445329.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9373..11685)
                     /gene="pbpC"
                     /locus_tag="ACHY5W_000363"
     CDS             complement(9373..11685)
                     /gene="pbpC"
                     /locus_tag="ACHY5W_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFS4445330.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDFTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLTLDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11686..16647)
                     /gene="yfhM"
                     /locus_tag="ACHY5W_000364"
     CDS             complement(11686..16647)
                     /gene="yfhM"
                     /locus_tag="ACHY5W_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFS4445331.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKALTLLDLSEVQLDGAVTLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRLLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKARTKNGEKPKQVNVLVSAVDSGVLNITDYVMPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFSAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIIGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDLGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVSNSGDQPLWLRVDASGYP
                     QSAPLPVNNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            16854..17699
                     /gene="sseA"
                     /locus_tag="ACHY5W_000365"
     CDS             16854..17699
                     /gene="sseA"
                     /locus_tag="ACHY5W_000365"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFS4445332.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            18042..18167
                     /gene="timP"
                     /locus_tag="ACHY5W_000366"
     CDS             18042..18167
                     /gene="timP"
                     /locus_tag="ACHY5W_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFS4445333.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNQSLSGNNLLVVNNLQNSR"
     gene            complement(18199..18975)
                     /gene="sseB"
                     /locus_tag="ACHY5W_000367"
     CDS             complement(18199..18975)
                     /gene="sseB"
                     /locus_tag="ACHY5W_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFS4445334.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19118..20401)
                     /gene="pepB"
                     /locus_tag="ACHY5W_000368"
     CDS             complement(19118..20401)
                     /gene="pepB"
                     /locus_tag="ACHY5W_000368"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFS4445335.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGIILHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDVDRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20646..20846)
                     /gene="iscX"
                     /locus_tag="ACHY5W_000369"
     CDS             complement(20646..20846)
                     /gene="iscX"
                     /locus_tag="ACHY5W_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFS4445336.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20858..21193)
                     /gene="fdx"
                     /locus_tag="ACHY5W_000370"
     CDS             complement(20858..21193)
                     /gene="fdx"
                     /locus_tag="ACHY5W_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFS4445337.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21195..23045)
                     /gene="hscA"
                     /locus_tag="ACHY5W_000371"
     CDS             complement(21195..23045)
                     /gene="hscA"
                     /locus_tag="ACHY5W_000371"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFS4445338.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALVADAALLSAAERQVIDDAA
                     AHLSEVAQGDDIDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(23062..23577)
                     /gene="hscB"
                     /locus_tag="ACHY5W_000372"
     CDS             complement(23062..23577)
                     /gene="hscB"
                     /locus_tag="ACHY5W_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFS4445339.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23673..23996)
                     /gene="iscA"
                     /locus_tag="ACHY5W_000373"
     CDS             complement(23673..23996)
                     /gene="iscA"
                     /locus_tag="ACHY5W_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFS4445340.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(24013..24399)
                     /gene="iscU"
                     /locus_tag="ACHY5W_000374"
     CDS             complement(24013..24399)
                     /gene="iscU"
                     /locus_tag="ACHY5W_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFS4445341.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24427..25641)
                     /gene="iscS"
                     /locus_tag="ACHY5W_000375"
     CDS             complement(24427..25641)
                     /gene="iscS"
                     /locus_tag="ACHY5W_000375"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4445342.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25753..26241)
                     /gene="iscR"
                     /locus_tag="ACHY5W_000376"
     CDS             complement(25753..26241)
                     /gene="iscR"
                     /locus_tag="ACHY5W_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFS4445343.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26602..27342)
                     /gene="trmJ"
                     /locus_tag="ACHY5W_000377"
     CDS             complement(26602..27342)
                     /gene="trmJ"
                     /locus_tag="ACHY5W_000377"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFS4445344.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27461..28264
                     /gene="suhB"
                     /locus_tag="ACHY5W_000378"
     CDS             27461..28264
                     /gene="suhB"
                     /locus_tag="ACHY5W_000378"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFS4445345.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28409..29263
                     /gene="yfhR"
                     /locus_tag="ACHY5W_000379"
     CDS             28409..29263
                     /gene="yfhR"
                     /locus_tag="ACHY5W_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4445346.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAKSVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYAIIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29454..30734
                     /gene="csiE"
                     /locus_tag="ACHY5W_000380"
     CDS             29454..30734
                     /gene="csiE"
                     /locus_tag="ACHY5W_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFS4445347.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30726..31865)
                     /gene="hcaT"
                     /locus_tag="ACHY5W_000381"
     CDS             complement(30726..31865)
                     /gene="hcaT"
                     /locus_tag="ACHY5W_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFS4445348.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(32025..32915)
                     /gene="hcaR"
                     /locus_tag="ACHY5W_000382"
     CDS             complement(32025..32915)
                     /gene="hcaR"
                     /locus_tag="ACHY5W_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFS4445349.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            33051..34412
                     /gene="hcaE"
                     /locus_tag="ACHY5W_000383"
     CDS             33051..34412
                     /gene="hcaE"
                     /locus_tag="ACHY5W_000383"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFS4445350.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            34409..34927
                     /gene="hcaF"
                     /locus_tag="ACHY5W_000384"
     CDS             34409..34927
                     /gene="hcaF"
                     /locus_tag="ACHY5W_000384"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFS4445351.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            34927..35247
                     /gene="hcaC"
                     /locus_tag="ACHY5W_000385"
     CDS             34927..35247
                     /gene="hcaC"
                     /locus_tag="ACHY5W_000385"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFS4445352.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            35244..35672
                     /gene="hcaB"
                     /locus_tag="ACHY5W_000386"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /pseudo
     CDS             35244..35672
                     /gene="hcaB"
                     /locus_tag="ACHY5W_000386"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
     gene            complement(35600..37168)
                     /gene="yphE"
                     /locus_tag="ACHY5W_000387"
     CDS             complement(35600..37168)
                     /gene="yphE"
                     /locus_tag="ACHY5W_000387"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS4445353.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSGLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITYPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAIDSVKIMLPS
                     LAINAGAQAFAPSR"
     gene            complement(37191..38174)
                     /gene="yphF"
                     /locus_tag="ACHY5W_000388"
     CDS             complement(37191..38174)
                     /gene="yphF"
                     /locus_tag="ACHY5W_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4445354.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38271..41552)
                     /gene="yphG"
                     /locus_tag="ACHY5W_000389"
     CDS             complement(38271..41552)
                     /gene="yphG"
                     /locus_tag="ACHY5W_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFS4445355.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHGLNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLKEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            41670..42863
                     /gene="yphH"
                     /locus_tag="ACHY5W_000390"
     CDS             41670..42863
                     /gene="yphH"
                     /locus_tag="ACHY5W_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4445356.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLAEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(43061..44314)
                     /gene="glyA"
                     /locus_tag="ACHY5W_000391"
     CDS             complement(43061..44314)
                     /gene="glyA"
                     /locus_tag="ACHY5W_000391"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4445357.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQRVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44642..45832
                     /gene="hmpA"
                     /locus_tag="ACHY5W_000392"
     CDS             44642..45832
                     /gene="hmpA"
                     /locus_tag="ACHY5W_000392"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFS4445358.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45877..46215)
                     /gene="glnB"
                     /locus_tag="ACHY5W_000393"
     CDS             complement(45877..46215)
                     /gene="glnB"
                     /locus_tag="ACHY5W_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFS4445359.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46276..47610)
                     /gene="glrR"
                     /locus_tag="ACHY5W_000394"
     CDS             complement(46276..47610)
                     /gene="glrR"
                     /locus_tag="ACHY5W_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFS4445360.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQTRLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47600..48313)
                     /gene="qseG"
                     /locus_tag="ACHY5W_000395"
     CDS             complement(47600..48313)
                     /gene="qseG"
                     /locus_tag="ACHY5W_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFS4445361.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDALRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(48478..49905)
                     /gene="qseE"
                     /locus_tag="ACHY5W_000396"
     CDS             complement(48478..49905)
                     /gene="qseE"
                     /locus_tag="ACHY5W_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFS4445362.1"
                     /translation="MKRCPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50481..54368)
                     /gene="purL"
                     /locus_tag="ACHY5W_000397"
                     /gene_synonym="purI"
     CDS             complement(50481..54368)
                     /gene="purL"
                     /locus_tag="ACHY5W_000397"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFS4445363.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLDDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIYADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT        12762 a        15163 c        13717 g        12960 t
ORIGIN      
        1 ttaaaccgtg accatgctgg cagaaataat ccaccgcacc acacgctttt aatacctgca
       61 aaggttggtg gcagtcagga caaagagcct ggactatgaa atctttgtta cacgttgcgc
      121 agtgagtagc ctcgccatta cgttcgagag ggttttggca gattgggcag ctgagttcca
      181 taaatgatcc tcacattttt atggatttcc ctctccatat ggaaagggaa accagataag
      241 taattactta cttttcttga tatgcttcat caggcgctta cgcttgcgca tctgggtcgg
      301 cgtcagggtg ttacgcttat tcgcatacgg gttttcccct tctttgaact gaatacgaat
      361 cggcgtcccc atcacatcca gagacttgcg gaaatagttc atcaaataac gcttgtaaga
      421 atccggcagg tcttttacct ggttaccgtg aatcaccaca atcggcgggt tataaccacc
      481 agcgtgggca tatttcagct tcacacgacg accgcgtacc agcggcggtt ggtgatcttc
      541 aacagccatc gtcatgatgc gcgtcagcat agaggtcccc acacgacggg tggagctgtc
      601 atacgcttca cgtactgatt caaacaagtt accaacacca ctgccgtgca aggcagagat
      661 aaagtgcaca cgagcaaaat cgataaagcc cagacggaag tccagcgttt ctttcacctg
      721 ctctttcact tcctgactca ggccatccca cttattcacc acaatgacaa gtgagcgccc
      781 actattgaga ataaagccca gcagcgagag atcctgatcg gaaatacctt cgcgcgcatc
      841 aatcactaac atcaccacgt tggcatcttc aatggactgc aacgttttga ttacggagaa
      901 tttctctaca gcatcggtga ttttgccgcg tttacgtacg ccagcggtgt caatgagcac
      961 atactcacgt ccatcgcgtt ccattgggat gtagatgctg tcacgcgtcg tgccaggcat
     1021 gtcgtaaaca acaacgcgct cttcaccaag aatacggtta gtgagtgtag acttacctac
     1081 gttcggacga cccacaatcg ccagtttgat cggcagactt tgcgggtcga agtcgtcttc
     1141 ctcttcttct tcgccgttct cttccgcttc aaattgcgcc cagtattcag cgtcttcgtc
     1201 gacttcctct tgcggtgcga gatcttccat ccacggcagc agcacatgct ccagcagact
     1261 taatacgcca cgaccgtgag acgcggcgat cgggtagatt tcacctaaac caagcgagta
     1321 gaaatcaacc actgcctgat cgggatccag accgtcagtt ttgtttgcca ccaggaaggt
     1381 cggtttttca cgggagcgca gatgtttggc aatcgcttca tctgccggca tcaggcccgc
     1441 gcgcgcatcc accataaaca gtacgacgtc cgcttcttca atcgccagca gcgactgttc
     1501 cgccatgcgg gtttctacac cgtcttctgt gccatcaatc ccgccggtat caatacagat
     1561 aaactcacgg ccttcaattt ccgcacgacc gtacttacgg tcacgagtca gacccgggaa
     1621 atccgcaacc agcgcatctc gggtgcgagt tagacggtta aataacgtgg attttcctac
     1681 gttagggcgc ccgacaagcg cgaccacagg taccatgttt aaagcctcat ttttataaat
     1741 catcagacaa cgcacgctat attcgcgtcg ttgttaaaaa caggaaaacg gcccctgtcc
     1801 aggagccgtt ttcaaagtga acggcagaga cgattaacgt gtaatagagt acacggttcc
     1861 gtcttttgcc tggatcagca gtttgccgtc agcggcaacc ggttcagtct ggaaaccgga
     1921 actatcaact ttttgctggg caacgaaacg accatcttcg acgttaatcc agtgcagata
     1981 accttcactg tcaccgacca ccaggttgcc attatacagc accggagaag tcagcaggcg
     2041 atgtagcaga tcactttgtg tccacagcgt aacgccgcca tcaatggtca acgccatcac
     2101 ccggtcattt tgatcgacca gatagatgcg attgccgtcg acgatgaaat cattcaccga
     2161 acccagttcg cgtttccaca taatctgacc actgcgcaga tcaagcgccg tcaggttacc
     2221 attataggcc agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt caacatcgct
     2281 cagacggtca atttcggtag aaccggtcgc ctgggaaata cgctgctgcc aaatcatctg
     2341 gccctgttcc atcagcactg cgctgacgcg accattatcg ccccccacga cggccgcacc
     2401 aaaagccgtt gccggcgcag actcgccacg caaagagagc gaaggcatat cgaggttaac
     2461 tgtccatttg acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt tactggtgtg
     2521 gattaacacc agaccgtcgc tgaccaccgg gcgcgaaagt gcttcacccg cgactttagt
     2581 ttgccatgcc acagtaccat cgctggtatt cagcgcgtaa acctgcgcct tttcgctgcc
     2641 aatgtagaca tgcccaccag acacggtcac accgccagaa agtaatgcag gctctttaga
     2701 gaaccagcca tctttctcgg ccaggctgac agaccagatt tctttgccat catccgcatt
     2761 cagcgctttt actaaaccag cgcggtccgc tgcatagaca acgttgtccg ccagtgccgg
     2821 atgaagattg gaatagaagt tgccaatgcc gctaccaacg gaagtgctcc acgccgtggt
     2881 cggcgtaaac tggttttcaa cggttggcaa tggggacatc tttaccacat cttcttcgct
     2941 gttaaacagc gaacagccgc ttaaaagggt aacggaaagc agtcctggca gcagtaattt
     3001 acgcaattgc atcgggtccc tctcagatgg acaaattatt aattttcatc tgcatcattt
     3061 cgctcagtgc cggagtaaca tcgcttttca cgcctgcttc ccatgcacta cgcgcacctt
     3121 gcttatcacc tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg gcagcccacc
     3181 cttcaccttt gatggtatca agggttttca gcgcggcatc agcctgcttg agctgtacct
     3241 gaacgcgagc aagacgcaga tttatcacgg ctttgagatt ttcatcgctc gtgtctgcca
     3301 gcccctgttg taactgggcg gcagctttct ctagttcatt tttgtcaaca aattgctgcg
     3361 caagttccaa agaagccagc gcaccataag tatttttatt ttcagcagca aatttttccg
     3421 ccgccgggat gctatccggt ttgccttcgc tcactgcggt aaccgcattt tgataggcaa
     3481 gagaagcgga gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag ccaatcagtg
     3541 cgccaacgcc caaaatcacc ccaacagcca gtgctttgcc attttcagca aaaaagcgtt
     3601 taaccgcttc tacctggtcg ttttcgttct cgtaaatttc cacgctgtcc ttctccttcc
     3661 ttaacccagt aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg ccgtttgctc
     3721 accagagcgc aaatccttca ctactgctgt gccgttagcc acttcagact cacccagcac
     3781 cacagcaacg cgggcacccc atttatcagc acgggcaaac tgtttcttaa agttgccgcc
     3841 gccgtggttg gtcatcaatt tcacgcccgg taattcatca cgcagacgct cagctaatgc
     3901 catagccgca gattgtgtat cagcacctga agccaccagg tatatatcga caacaggatc
     3961 ggctttaaat tccggattaa cggcctgtac taacaataca agacgttcga ggcccatcgc
     4021 aaaaccgaca gccggtgttg cacgaccgcc cagttgttcc acaagaccgt cataacgacc
     4081 gcctgcacac acggtgccct gggagccgag actgttagtc acccactcga aaacggtacg
     4141 gttatagtaa tccagaccac gcaccagacg ctggtttacg gtgtaagcga tccccgcgct
     4201 ctccagcagt ttgcacagac cggcaaaatg ctcacgagat tcctcgtcca gataatcacc
     4261 taatgccgga gcgtcgttga gaagcgcctg cacttccgga ttttttgaat ccagcacgcg
     4321 cagcgggtta gtgtacatgc ggcgtttgca gtcttcgtcc agcttttctt tatgctgctc
     4381 aaggaatgcc accagcgcat cgcggtaatt ggcgcgtgct tccagcgaac cgatagaatt
     4441 cagctcaaga gttacgtgct cggaaatacc cagcgcgcgc caccagcggg cggtgagcat
     4501 aatcagttca gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc ccaactgatg
     4561 gaactgacga taacgccctt tctgcggacg ctcgtgacgg aacatcggcc cgatatacca
     4621 cagacgctgt tcctgattgt acagaagacc atgctcgatg ccggcgcgta cacagcccgc
     4681 cgtcccttca gggcgcagag tcaggctgtc gccattgcga tcctcaaagg tgtacatctc
     4741 tttttcaacc acgtcggtga cttcaccaat cgcacgtttg aatagcgggg tctgctctac
     4801 aatcggcaag cggatttcac tgtaaccgta gctgccgagc acgtttttca gtgtgccttc
     4861 aatgcgctgc cagatggccg tttcgccagg caggtaatcg ttcatgccgc gaatggcttg
     4921 aatgtttttt gccacgttta ttctctttct gaatataaaa atgaaccctc aacgcttccc
     4981 tcaatgtttc gggagctatg cgggttcaat catacacggg aagcgaggcg cttcccatca
     5041 cgttattatt tttcaacctg ctgaacgtca attcgacgcg cttcgtccag ctgactggct
     5101 ttcgcacgaa tgcgtgcttc cagctggtcg atcatatcgt tgttgtccag acggtctttg
     5161 cgcacgccat cttcatagag gccgcttttc ttgttgccgc cggtgacgcc gagtgtagaa
     5221 accagcgcct cacctgggcc attcaccacg cagccgataa tcgaaacgtc catcggagtg
     5281 atgatatctt ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc aaactcctga
     5341 cgcgaacagg tcggacaggc gatgaagttg atccctcgcg aacggatacg cagcgatttc
     5401 aaaatatcga aaccgacttt gatctcttcg accggatcgg ccgccagtga tacgcgcagc
     5461 gtgtcgccga tgccttcaga cagcagcaga cctaaaccaa tggcggattt tactgccccg
     5521 ctgcgcgcgc cacccgcttc ggtgatcccc agatgcagcg gctgatcgat ttgttttgcc
     5581 agcaaacgat aagactcaac agcgaggaag acgtcagacg ctttcacgct gactttgaac
     5641 tgatcgaagt tcaggcgatc gagatgatca acatgacgca tggcggattc cagcaacgcc
     5701 tgcggcgtcg gttcgccata cttttcttgc agatcttttt ccagcgatcc ggcgttaacg
     5761 ccaatacgga tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg aatacgctct
     5821 tcattaccga tattgccagg gttaatacgc agacaatcga cgccgtattc cgctactttc
     5881 agcgcaatgc gatagtcgaa gtggatgtca gccaccagcg gcacgttaac ctgctgtttg
     5941 atgagtttga acgcttctgc cgcgtccatc gtcggtacgg agacacggac gatatcagcg
     6001 ccaacgcgtt ccagcgcctt gatttgattg accgttgctt cgacatctgt cgtacgcgta
     6061 ttggtcatgg actgtacggc gatgggagca ccatcgccaa tcggcacatt cccaacgtaa
     6121 atacgtgttg attttctacg ttgaattgga gcctggttat gcatgaaaaa tctcccgcgt
     6181 tacccgtctg ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc gcaacctggt
     6241 tagttctgat aaaacgactc agatcgacag gtttcccttg atactggatc tgtactgcgg
     6301 ctggcgcacc aattttcagt ttgtacggtg cctggccggt taagttcaaa ttaccgtctt
     6361 tacgctgcat accgctaaac aattttttac cggtagcatc agtgacctcc agccagcaat
     6421 cggcagtaaa gttcatcacc agcgcattcg gatcagcaac cggcgtggtc acgccagcct
     6481 gatcggttgg caacggcgca gcaccatctg gcgttgttgc tgccgttggt gccggggtcg
     6541 cggcggtatc aacatttgcc tgcgaaggcg aaacaaccgc attctgttgc ggatcaacag
     6601 ctggtgctgg cgcagttacg gcaggtgttt gcgtgttggt tgcggtagta tccacagacg
     6661 ctggcggcgt ggacgttgta gccgggtctg tagttgtcga cgtatttaac ggaacactct
     6721 gcccctgctc gctattactg ctcagttccg ccgaagattg atcggccata gtggtgatct
     6781 cttcctgctg agctttgtgg tcttgccacc accaggcacc gctcaggccg ataaccacaa
     6841 acaacaccag ccaggtgaag gtcatcagcc agccgtcgcg ttttttgcgg cgtttaccga
     6901 gagaaaaact ctgcatcggc gcaacttttg cagcccgtag tggagcctgc ttttccagcc
     6961 ctggcagcag ttcttcttct ggaatatgta ccagacgcgc ataagagcgg atatatccgc
     7021 gcaggaatgt tgaagcaaga tcggcgggtg ccttatcttc ttcaatgtcg cgtaccgtgg
     7081 aaaccttcag gcaaagtcgc tcggcaacgg cctgctgact aagtcctagt tgttcgcgag
     7141 cattacgcag gcgagcgccg gtagtaagtg cttcattttg gtcgtgcgtg gcttcagtat
     7201 tcattcgctg caggtacgtt taaatgagaa ttgagatgcc ggtgaaccat catgctcacc
     7261 cacaccgcga aacatccgtt aagttaaccg ttatcataca gtataagacc gtctgtgtgc
     7321 tcgtgacaaa gcccacattc actaaggcta acttactgtt gcatcgttac atactgcctt
     7381 aaagtcagca aaaacgcacc gttaatattg accagacaat tgcaacttaa tacttcattc
     7441 ataaaacatt acgccacggt acataaagta accgtggcgt aatggctatc agaccgcttt
     7501 aatgtcgatg gcttcaccct gcatccgttt acgcagggta cgtttcgtac ggtcgataac
     7561 atcgcccgcc aactgaccac aggcagcatc gatatcatca ccacgcgttt tacgcacaat
     7621 agtggtgaaa ccgtagctca tcagcacttt tgagaaacgg tcgatacggc tgttcgagct
     7681 gcgtccatac ggcgcacccg ggaacgggtt ccacgggatc aggttgatct tacacggcgt
     7741 atctttcagc agttccgcca gttggtgagc gtgttcagtg ccgtcgttaa cgtggtcaag
     7801 catcacgtat tcaatagtga ctcggccctg attggcgttg gatttctcca gataacggcg
     7861 caccgcagca aggaacgttt cgatattgta ctttttgttg atcggcacaa tttcatcacg
     7921 aatttcgtcg ttcggcgcat gcagggaaat tgccagcgca acatcgatca tatcgcccag
     7981 tttatccagc gctggaacta caccggaagt ggaaagcgtc acgcgacgtt tagacagacc
     8041 aaaaccgaaa tcatcaagca tgatttccat cgccggaacg acgttgttca ggttgagcag
     8101 cggctcgccc atgcccatca tcactacgtt agtgatcgga cgctgaccgg tgacttttgc
     8161 tgcgccgacg attttcgccg cacgccacac ctggccgata atttccgata cccgcaggtt
     8221 gcggttaaag ccctgctgtg cggtggaaca gaatttacac tccagcgcac accccacctg
     8281 cgaagagacg cagagcgtag cgcggtcgtc ttccgggatg tacaccgttt cgacacgctg
     8341 gtcacccacg gcaatcgccc atttaatggt gccgtcagat gaacgctgtt cttcaaccac
     8401 ttccggtgcg cggatttccg ccacctcttt cagcttgccg cgcaacactt tgttgatgtc
     8461 agtcatctca tcaaaattgt cgcagcaata gtgatacatc cacttcatca cctgatcggc
     8521 gcggaagggt ttttcaccta aatctttaaa aaactcccgc atctgctgac gattgagatc
     8581 cagcaggttg atttttccat ctttggtggt gacgttttca ggtgtgacta attgttcaga
     8641 catatgctat tccggcctcg ttattacacg ttatggcccc tggagggttg aaaaaagaaa
     8701 cgccccggtg agcggctgct cgtccggggg cgctgcattg tacaaattct ggcgcacgga
     8761 tgccacgttt gcacgcggca tttacgaaat tattaacggg tgcgcgggca cacttcgcct
     8821 tcgccgaaga aataagcgat ttcgcgagcg gcagattcga cggagtcaga accgtgggta
     8881 ccattttcgg tcaggctgtc agcgtaatca gcgcgcagag taccggccag tgcgtttgcc
     8941 ggattggtcg cgcccagcag atcgcggtga cgctgaacgg cgttttcacc ttccagcacg
     9001 gaaaccacga tcgggccaga ggtcatgaat tcaaccagac catcaaagaa cggttttcca
     9061 tcgtgttcag cataaaagcc acgagcctgt tcaacggtca ggtgcagcat tttggtgccg
     9121 acaattttga accctgcagc ttcaaagcgc gcaaagatat taccaatgac gttttttgct
     9181 accgcgttcg gtttgataat ggaaaaagta cgttcaatag ccatttttac ctctgtaaat
     9241 tgttctgttg ttgtctgtac cagcgtacga gatggcgcgg attataatga gcaacagagc
     9301 cgttgactat tgataaaggt aacatatttt taaaataaac ctagtttaag caacaatcag
     9361 ggacaaaaca gactattgca tgacaaattt cactgtcgct atttgcccta cgtcatccat
     9421 caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc gcccgcgttc
     9481 agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac ttgattgcaa
     9541 cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atctcgccat cgcgaacgcc
     9601 tgtcagctgc agcgggagct gggcatcgtg accgtatggc gggcaaattg tcgaggccgg
     9661 tggtaagcgc acagcgcgac gttctgatgc gggcaaccag ggttccagcg gtagcggcca
     9721 gacgtttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt tgccattttc
     9781 atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca gtaatagagt
     9841 tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt taccgtcacc
     9901 ttcaggcaaa gactgtccgc ccggccagca gataacgcca cgagtgactg agttcgggcg
     9961 cgggtcttcc ggcagattag cactgcgcga cagtaagata ttattgacct gattcaacaa
    10021 tggtacggca ctggcaaagc caaactgacc aacaacgggc gtgccgtccg gtctgccagt
    10081 ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac gatagccata
    10141 gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcaccat ccggcaaggg
    10201 ttgcgcttca tcagccataa tccggcgaat gatccacgca gcccccgacg acattaaagg
    10261 ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc cgtggcgagc
    10321 aaacgcggta tacgctgccg ccatatcttc cagttttgca ccagcaccgc cgagaatgag
    10381 cgaaagattg ggcgcagcgc cgttgggcag atacaacggc aatccaacat tgcgtaattt
    10441 tgccgcaaac cgtttcggtc cataggcttc cagcacctgc acagcaggta aattcagcga
    10501 gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctatcaa agttacctgg
    10561 tcgataatca cctgtgcgtc ggggaacgtc ttgcaacagc gatgccgggt ggatcaagcc
    10621 ttcatccagc gtcagaccgt aaacaaacgg tttgagcacc gatcctggcg atcggatcgc
    10681 attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg aacccaccca
    10741 gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc tgcgcggtgg
    10801 taatcgtcct ttccagtttt gcgccagttc ttccagacgt cgttgaagac cggcatccag
    10861 cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa acagcggtgc
    10921 caattgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact cttttacctg
    10981 ctcacgggac cacacacctt gcgcagccat ccgttcgagc actttattac gcgcggcttc
    11041 ggcacgctcc ggccaacgat ccgggcgcag tcggctgggc gcttgcggta acaccgccag
    11101 cattgccgcc tcggaatagc ttaaattcgc aggcgatttt ccgagataag cccaacttgc
    11161 cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca aggtcagaat
    11221 ttcacgctta gacaaatgcc actccagttg caacgcgcgc cagagctggc gaattttgcc
    11281 gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga gcgtgctgcc
    11341 accggaaata acccgtcccg aagtgaaatc ttgccatgct gcgcgcgcca ccgagaatgg
    11401 attcacccca ggatgcttcc agaaccagcg atcttcataa ttgatcagcg cttcaaggta
    11461 acgtggagaa acatcttcga ttgttaccgg ataacgccag atgccgtcag catcggcgaa
    11521 gcgccagagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga cttcatgcag
    11581 cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa atggcgcagc
    11641 tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg gtctgacaat
    11701 cagcaaatct tcagccgcgc cggtcgcccg ccattgggga acatacattg attccaccat
    11761 cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca ccagcgttac
    11821 cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga actcaatgtg
    11881 cttaatgctc gcctgctgca tctggttcag taagttctgc acttcgccac cactttgctc
    11941 caggctggcg ctaccgttcg ccagattctg gttttccagt tccagacccg caggcagcag
    12001 atccacgact aacgcatccg gcacgctgtt actggctttt acctgcaacc agaccagcac
    12061 cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac caagaatatg
    12121 acgctcgatt tgcagcacat tgttcaccgg taaaggtgcg gattgcggat aaccgctggc
    12181 atccacacgc aaccataacg gctgatcgcc actgttgctc acctgcaagg tggcaagttg
    12241 atcgctattc agattgctgt tttgcgcttt ctcgcctgtc agcggctcag ctgagaaaga
    12301 ggtttgcgcc tgccatttac ccggtaaatc ctgaatcgta cgggcagcca ggaacaacgc
    12361 gttactttcc tgcgtcgata gccagcgttc accaaacgcc tgctgcgaaa gtgtgttcag
    12421 caaagtgtac tgctcatcgg gtagcagttt attttcttcc agcaaggaga gcattaacgc
    12481 gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac tattacgcgg
    12541 cgttttcagc gccagcacaa ttgcttcttc accacgcgtc gcatcaccca tggttttcag
    12601 cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgcacgat gctcccagat
    12661 ttcacgcagc gcacccagcg gagccttttg ctgacgggcc agcaccagcg cagcgtaaga
    12721 ctgtacggcg aatttactgg ctttgagatt atccgcgtac gggatcgaca tcatgcctag
    12781 atcttgtaaa tagcgcagca gacgctcatt accccggtta atggcgtctg tcggcacgct
    12841 gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg taagccgtca gccagtactc
    12901 ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta tcacgttgca tttgcagcaa
    12961 acgggaaatg ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc ctatgatgcc
    13021 caacgcctgc agttgggctg cgttggtata aagtgacggg aacaggccgc tggcggtttg
    13081 ctcaagacag ccgtacggat acgcttttaa ctctttgata taacgtgcga tgttcagcgg
    13141 tggtttgccg ctcaacaaca attgcccttc cagcgtaaca ggcgagaagt tttgcaatcc
    13201 atccgccgga atcgcccatg tctcaccagg ctgcaacgcc gtaccgtaat taaccgtttg
    13261 tgccgagaac gccggacgga cgccgatttt ccactgctta tgctgatcgg caacggtttc
    13321 acccggtaac gctaacccgc taatggtggc ctgaatttct ccatcgccat aacccggcaa
    13381 tgctcgcacc gggataaaca gcgtagtacg cacacctggc gctaattcaa cggctgcggg
    13441 tgaatcgctg accagttcaa gcaaaccact ggcggtcagg gcaacgttca gtttttgcgg
    13501 tttatcggta agattagtga tatccagcgt cagacgcgag gtatcgccac tcgccataaa
    13561 gcgcggcatg ttcagttcag caatcaccgg tgcggcaact atcactttgc tttcgttgct
    13621 accgaagtca tctgccgtcc aggcttgcgc catgacgcgc aattcaccgt taaaatcgcc
    13681 aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc gttaccggca gcgcctgctg
    13741 cgcgacaata ttgacgtgat tgaccggcgg tttaccacca cgtttcagct catcaccatc
    13801 gccaccgaaa cgcagagctg ccagacgccc ctgaccttca ataacctgac cgtaaatatc
    13861 gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc tgccacggat ctggcatgac
    13921 gtagtcagta atattcagca caccactatc aacggcagac accagcacat tcacctgttt
    13981 aggcttctcg ccatttttag tgcgggcttt aattttcacg gttaatggtt gattgggacg
    14041 catttttgct ggtgtttcca gcgccagatc gaggcgacgg ttttcatcac caagcggcag
    14101 atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga gatttatcgc caggacgcac
    14161 caccagcgta ctcaaataaa gatcatgacg attccaggtt ttatcgaccg gaatcgtcag
    14221 atccagccct tgagccggaa catcaatctc ttgccaccac agcggccctt cactggactc
    14281 gaccatcgca taacctttac ccgccgttgg cgcggcaata tgcaacttaa tggtatcgcc
    14341 agggcgataa ctggctttat ctagtttcag cgtgacacgg tcgggtcgca cggcaccgct
    14401 cccgtcgctg ttgtcctgcc agctatagcc agcccagaaa cgcacactac tgaccgcttc
    14461 attcggcgct ttgacttcca gacgataagc gccccactct accgggaaac tgaccttgcc
    14521 ggtttcgtcc gctttcagat ccagagtttg ttcattttca atcagatctt tttgatcaaa
    14581 ctgtgattgc cagccttcat cttctgacca gttccagtag taatcgcggc gttcgcgaat
    14641 caggcgcacc tgcaatcccg acacggcttt tttcacgcct tgcgcatcgc tataaacgat
    14701 gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc tgtcagtgcg
    14761 ataatcgtaa accgatttcg aggcgaactg cggacggagc cccggcaatg catcagcagg
    14821 ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg cccgattcca gcaaactgcc
    14881 ctggaaaata acctgcaatg gggaatgcgt ttccttccac tggctttctg tagaaacttc
    14941 gccgcgccct ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg aaaggttttc
    15001 ggcagctata tcgccgaatt caaaaccagg taaggctgac acagcctcac gcagtaggcg
    15061 caggaaaagt tgcccttgca aagtattacc attagcaggc gcaccgtaca ggtagtatcc
    15121 caccacggag aatttcactt catcattcgg cgtaagcggg gttttctcac cggtcagatt
    15181 cagcgccata cgctctggca taaaatcttc gacgtggaaa tcccacatcc gatactggtt
    15241 atcgcccgtg ttagcgcgaa tatgccacat accggttgcc gcattgctat cgagtggcca
    15301 ggtaaagtgg tagaggccat tttccggctg actaacgacg ctcctgagta cctgcccatc
    15361 gggtttaatc acgtctaact tgatgggttg attgggcaac gctttaccgt ctgcatcacg
    15421 cagcaaacca ttgaggatta ccgtttcacc cgggcgataa agatcgcgcg ggccaaacat
    15481 gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta aattctgcta agtccagcgc
    15541 cggaagtttt aaatcgagta gcgttgtctg accgtcttta cgtgccagca ataatgcggc
    15601 gtttttatca ttttccagct gcacatgccc ctgagcgtca ctggttgcct gagtcagagt
    15661 ctgccctttc tcatttaata aagagacttc aattccttgc tgggccgcgc cgttttccag
    15721 actttgggta aagatatcca gacgattgtg ataacggtga gctgaaacgc cgatatcact
    15781 taacgtaaac agcgtcgcgg gattactgta atcgtaacgt ccagcctgat tcatcacagc
    15841 cagatacacg cccgcctgct gaagcggttt gatatcgccc agcggcagca ataatttttc
    15901 acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg tagaccagat ccgccatctg
    15961 cagcagtttg tctgactgcc agttcgccag cgaattgcgg tattcccatt ggctaatgaa
    16021 tgctggcaga gattctggct taacgcggaa gaagttaaca tcgacattat taacgttgag
    16081 cgccattacc ggcagccctt caacgacttt accaggcagc agcgaaccac ggctggcaaa
    16141 accgacgctg ggttggatgt cgcgggtagt tatagttttt tcgtaatctt tactgaaggt
    16201 tgcgttgttg agtgctttga cctccttgcc aatagtaacg atcaaatcac gtttcggttc
    16261 gaggtggcgt aaacgcagct ctttaagatt atctgacagc tcccaggcac catccacttt
    16321 gccgcttttt ttatcgacga catgaataac gcgtgagaaa tcctgatccg ggtcgagagg
    16381 gatggagaac gtcagcacca gcgtgactgc accatcaagt tggacttcag agagatccag
    16441 caatgtcagc gccttaccgg cactctgttg tgccagtttt tgtcgctccg gcacggagag
    16501 ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta acttcagaag gcgcatcttt
    16561 tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca gcattagcat
    16621 gcaggcggct acgcgtaact ttttcatttt tcatccctgc cacaatggcc cgttagcaac
    16681 gtcgaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac gcccatacaa
    16741 tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttt gcttgtcgct
    16801 tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg cctatgtcca
    16861 cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg gaaattcaga
    16921 ttatcgatgc ccgcatggcg tcgcctggac aggaggatcg taacgttgct caggagtatc
    16981 tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct gatcacactt
    17041 ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg cgtgaattag
    17101 gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc tcagccccac
    17161 gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg gggggtggac
    17221 ttgcaggctg gcagcgcgat gatctgttgt tagaagaagg tgcagtagag ctgccggaag
    17281 gagagtttaa cgccgcgttt aatcctgaag cagtggtgaa agtaaccgat gtgttattgg
    17341 caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt tttaacgcag
    17401 aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggtgca ttgaacgttc
    17461 cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg gatgcgatat
    17521 tttttggtcg cggcgtcagc tatgacaaac cgattatcgt cagctgcggc tctggtgtaa
    17581 cagcagccgt ggttttgttg gcactcgcga cgctggatgt gccaaacgtg aaactgtacg
    17641 acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca ttgaaataag
    17701 ctttttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg gtctggttcg
    17761 cggcctttcc agcaggttga cttgtgttgc cggagcaacg caggcacccc acagtgttga
    17821 aatactctct tttatgcctg gctatgtcaa gataataaac agagtaatta ttcaaacatg
    17881 agagtattat tcgtgacggc ccttttcgcc gtctcgcaaa cgggcgctgg ctttaggaaa
    17941 ggatgttcta tgaccgtaaa tgcaggcacc ccacagcgct tgctatcgcg gcaatatcgc
    18001 cagtggtgct gtcgtgacgc ggtcttcgca tggaccgcac aatgaagata cggtgctttt
    18061 gtatcgtact tattgtttct ggtgcgctgt taaccgaggt aaataataac cagagtctct
    18121 ccggcaacaa tttactggtg gttaacaacc ttcagaacag caggtaagcc cgaatgccgc
    18181 cctttggggc ggcatatttt agattatccg gttctgttta aagtcacgca aaaaaccacc
    18241 ccaacgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa ttcctttttc
    18301 cccttttttc acctgacaaa tatcgattgg ttcatcggca ggtaatgtat cggtcgctac
    18361 acttcccgcc gcctgaataa tttcttcgac atcaccatcg gcttcaatgc caataagtaa
    18421 attaggctgt gcctcttcgt tctctttaat tgaacatata aaagcacgct tcaccggctt
    18481 aatagtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct ctgcgacttc
    18541 cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg gatttccctc
    18601 ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg gcagtttggc
    18661 attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg gcattacgac
    18721 aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa aaaaaggaat
    18781 gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat cttcaaccac
    18841 agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta gagtacggaa
    18901 aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt caagttcatt
    18961 ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcacaag tgcctgatgc
    19021 gccatactta tcaggcctac aagggattca caatttgttg aatttgcaat actttgtagg
    19081 ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcaa
    19141 tcgtgcgcac accaagtccc gtagcacccg cagaccactg ttcaaccggc gctttacggt
    19201 aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca
    19261 ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt
    19321 cggcaaagtt agacggcagc tggctgcggt ggaactccgc cagcggcaga cgccagaatg
    19381 gttcgttctc ttgtgaggca ctcgccagca aacgaccggc cagcgcatcg tcaaaactga
    19441 acagcgcatg ataatcatta cccagcgcgg ttttcgccgc cccggtgagg gtcgccgcat
    19501 caatgatcag ttccggtttc tgcgcactgg catcaatcag gccatcggca agcaccagac
    19561 gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag gtgatgatat
    19621 cgcccagctt gaacgcatta ccgctaatca ggttatccgc acagcagagg aacagcttca
    19681 cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc
    19741 cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg
    19801 agtcaaaagt gataccttta ccgaccaggc acgcgtacac tggcgcttct ttatcgccag
    19861 ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt
    19921 gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac
    19981 gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca
    20041 attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat
    20101 ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc gtgcctttcg
    20161 gggctttgta accttgccag aatgcccagc agcgatccac atcccagcct tcaccgctta
    20221 gctgaacatg tttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagaccca
    20281 gatcgtctgc cccgttcaaa tgcaggataa tgccgtcatt attaatgctg taagttgctt
    20341 tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca
    20401 ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta
    20461 aatgattcgc gttgcaggac aaaacgcatt gcgttttgaa cagcgcttgc gctggccccg
    20521 gaggggtgag gccaaaaggc cgaatcacgt ggcaagtgag tgaagcccca ggagcataga
    20581 taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc
    20641 ggaaattatt cggcttcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg
    20701 gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg
    20761 aatcgaaccg ttttcggatc aagatcggga tacgcatcgt acagtgcttc gccaatttcg
    20821 cggctatcgg tccacttaag tcccatacta acctctgtta atgctcacgc gcatggttga
    20881 tagtgtaacg cgggatttcg accactaaat cttcgtcggt gacgcgcgcc tggcagctta
    20941 aacggctttc cggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc
    21001 tttccggcag tgagtcaaaa ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg
    21061 atttttcaca ggcgtgttca atctcgatac cgttacgcag cgctgcgtcg agaatggttt
    21121 caccgctatt agcttccaga acagcgccat caggacagag atcctgatga ggcaaaataa
    21181 caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac
    21241 tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt
    21301 tcgatggcgt caatatcatc gccctgcgcc acttcactca ggtgagcggc agcatcgtca
    21361 atgacctgac gttctgcggc gcttaacagc gcggcatcag caaccagcgc gccgtgcaga
    21421 ctttccagca cacgcgccgc ttctactttt tgttctgcca gcattcgggc ttttacgtcc
    21481 tgctcggcat agctcattga gtctttgatc atcgaagcga tttcgctatc ggtcagaccg
    21541 taagacggtt tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg
    21601 ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt
    21661 agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg
    21721 cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa
    21781 tcctgagcgc gggccaccgg aatagtggta ttacgcggaa tcactttctc caccaggccg
    21841 cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcaacagcat ttcgctgtct
    21901 ggcttgttac ccaccagaat atccgcctga atcgctgcgc caatagcgac gactttatcc
    21961 gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc
    22021 ggcacgcgag tagaaccgcc caccatcacc acttccagta cttcgtcagc ctcaacaccc
    22081 gcatctttca gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt
    22141 tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag
    22201 tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg
    22261 cggttatcgc tacgatcagg aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg
    22321 tcgaaatcat cgccgccgag cgcggaatca ccgccggttg ccagcacttc aaacacgccg
    22381 cgacttaagc gcagaatgga aatatcaaac gtcccgccac cgaggtcata aacggcgatc
    22441 acgccttcct gaccggaatc cagcccgtag gcgatagccg cggcggtcgg ttcgttaagt
    22501 aagcgcagga cgtgaaggcc cgccagacgc gccgcgtctt tggtgccctg acgctgggca
    22561 tcgtcaaagt acgccggaac ggtgataact acaccatcca gctcgcctgc cagggcttca
    22621 gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc
    22681 cccgccgccg tttcaatcat cggcaggccg ttttcgctgg cctggaattg ataaggcaga
    22741 tgcggatagc gttgctggat atcagccagc gagcgtccca tcaggcgttt aacagaacta
    22801 attgtgttgg cggtatcgag cgctgcatta gtacgcgcgt cataacccac cgaatgccct
    22861 tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt
    22921 tcggcctgac cgctgcgcac tgtcgccacc agcgagttgg ttgtgcccag gtcaataccg
    22981 gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag
    23041 gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact
    23101 gcttcgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccacgt
    23161 ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg
    23221 tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagctcttc
    23281 gcgcagctcc aactgttcca tcaggaacgc ggtgtcgcgc acagtatgtt gctcgctggc
    23341 gagatcaaag ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt
    23401 ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct
    23461 tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag
    23521 cgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac
    23581 attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc
    23641 aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca
    23701 cactcatctt taacgttcgg gttggtgaat ttaaaccctt cgttcaggcc ttcttttacg
    23761 aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac gactttcacg
    23821 cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc cagtacataa
    23881 gccatacctg aacacccgga ggttctcacg cccagacgca ggccaaaccc tttaccgcgg
    23941 ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc
    24001 aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat
    24061 cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc
    24121 aatatcggtg tttttgatcg cctgcgcttc gtcgagagac ttccctttca cccattcggt
    24181 gaccagggag ctggaagcga tagcggaacc gcagccgtaa gttttaaaac gcgcgtcttc
    24241 aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc
    24301 acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg
    24361 attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga
    24421 taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc
    24481 ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta
    24541 gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag
    24601 ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca
    24661 ggcggaaccg gaagaaaccg cgagatcttt cagcgccata atcagcgact caccttcaac
    24721 gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta
    24781 aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc gcagacgttc
    24841 catctcggtc gccatctctt cttttgcgat gcgataggct tcgcccattc cgacgatctg
    24901 gtgaacaggc agagtgccgg aacgcatacc gcgttcgtga ccgccgccgt gcatttgcgc
    24961 ttcgatgcgt acgcgcggtt tacgacgcac atacagcgca ccgatacctt tcgggccata
    25021 gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt cgataggcag
    25081 tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc
    25141 gccgatagcc gcaatatcct gtaccacgcc gatttcgtta ttcacgtgca tgatagaaac
    25201 gaggatggtg tcgtcacgca tcgccgcttc gagttctttc aggtcgataa taccgttacg
    25261 ctgcggtgcc aggtaggtga cttcgaaacc ttcgcgctcc agctgacggc aggtatccag
    25321 taccgctttg tgttcggttt tgctggtgat gatgtgcttg ccttttttct gataaaagtt
    25381 ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc
    25441 acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta ccgcttcttc
    25501 agcctgccag ccgaaacggt gagaacggga ggccgggtta ccaaaggttc cgtccatcgt
    25561 cataaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc
    25621 gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaatc
    25681 aggctaccgg ctggatgtac gaccgtgttt acggagcgcg atacgccccg gcctgattct
    25741 gaatactttt tattaagcgc gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg
    25801 cgcgtcgtga gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc
    25861 gcctaaagta atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc
    25921 gtgggtcagg catttatcgc cgccctgaca gccgccttta ccctgacaac gggtggcatc
    25981 tacagattcg tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc
    26041 taacagataa ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg
    26101 ggaaaacagt tgttccagat aagaaaggga aattccctga cgttcggaaa tatcagccaa
    26161 cggtaccggg cccgcttcag agttgagcgc aacatcaagc attgcggtca cggcatagcg
    26221 ccctttagat gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt
    26281 ttattgtaaa agtgggggta ttgcatagca gggtcaagtc tgacattccc gagtaaattg
    26341 gtcaactatt tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc
    26401 gtggcgtttg tacctgttgt cggatgcggc gtgaacgcca tatccgacct ggggttctgt
    26461 acactgtagg cctgataaga cgcattacgc gtcgcatcag gcaacggctg tcggatgcgg
    26521 cgtgaacgcc ttatccgacc tacggttctg ttcactgtag gcctgataag acgcgtaacg
    26581 cgtcgcatca ggcaacggct gttattcggc cttgttacct ttattctgct gctcaataga
    26641 agccagaatc ccgcgcagga tattcaactc ctggctttcc gggcgcgcac gggtaaacag
    26701 acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac cggttgccag
    26761 cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg gatacggcgt
    26821 ctcttcatgc tcgacctgct caccattttc ctgagttgcc agccaggcca tacgcacttc
    26881 ataggcaata acctgaaccg ccatcgccag gttcagcgag ctgtattcag ggttagcggc
    26941 aatcgcgaca tgataatgac acttctgcaa ctcttcattg gtcaggccga cacgttcgcg
    27001 accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca gaccgcattc
    27061 gcgcgggtcg agcatcggcc acggcagcgt gcgggaacgt gcgctggtgc ccaccaccag
    27121 gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac caatcacatc
    27181 gctggccccc gccgccaggg caatcgcctg ggagtcgggt ttcaccagtg gattaaccag
    27241 ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca tattgccggt
    27301 gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc ttcgtctaaa
    27361 gattattcgc gcatcttatc ataaaacgaa gacagatgcc gatctcgctg ctatactctg
    27421 cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga tgctgaacat
    27481 cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg aaaccccgga
    27541 cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag ataaagctgc
    27601 cgaagcggtg attatcgaca cgattcgtaa atcttaccca cagcacacca tcatcaccga
    27661 agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg atccactgga
    27721 tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat
    27781 caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc
    27841 cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggcagca ccgctcgcga
    27901 tctcgacggt actattctgg cgaccggctt cccgttcaaa gcaaaacagt acgccactac
    27961 ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc gtaccggttc
    28021 tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg
    28081 tctgcgcccg tgggacttcg ctgcaggcga gctgctggtt cgtgaagcgg gcggcatcgt
    28141 cagcgacttc accggtggtc ataactatat gctgaccggt aacatcgttg ctggtaaccc
    28201 gcgtgttgtt aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg
    28261 ttaatgactc aggcgggtga tatcactcac ccgccctcgc ctttcaggcg ctattccgaa
    28321 atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc ccgattaatg
    28381 gatgataaag aagtcgcaag gaaaggttat ggcactgcca gtgaacaaac gcgttcccaa
    28441 aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat
    28501 caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt cggcgaaatc
    28561 cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc cttcttcgac
    28621 gggccctgct gacaacgcca tcgcaaccat cattcatgct cacggcaatg ccggaaatat
    28681 gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg tttttatgtt
    28741 tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat tgctggacga
    28801 tacgcaaagc gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac aacggctggt
    28861 gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc agggtgatcg
    28921 tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa tcatcgccaa
    28981 ccagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg aaaattatat
    29041 cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc acgttatccc
    29101 atggcagcac agcgaaaagt tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat
    29161 cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt atcgcgaaca
    29221 gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga tcgctagcac
    29281 gataatcatt cacaaaacca ccttaagaca tgctaatcca ctgatcagaa cagtttaaga
    29341 tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc ttcgccattt
    29401 ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta cagatgatgc
    29461 ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag atcttgctga
    29521 cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt aatggtgtgg
    29581 atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg cgctatcatc
    29641 aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca gtgcttaacc
    29701 aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg tcatttatta
    29761 ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact
    29821 tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac
    29881 gctttgaatc gcgcgatatt catttcctgt gtctgtatct gcaatattgt ttgctgcagc
    29941 accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc
    30001 ttgaatttca ggtagcacag gaaattggac gccactggca gcgtcgggcg ctccagcctg
    30061 taccacctga tgagccactg tttatggcac tacttttttc catgttgcgg gttcccgatc
    30121 cattgcggga tgcgcatcag cgggacagac aattgcgtca gtctatcaaa cgtctggtaa
    30181 accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta tgtgatcagc
    30241 tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt attgataata
    30301 ccctaccgga agagttcgcc agactgtatc cacgcctggt acgcaccacc cgcgcggcgc
    30361 tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagt ggtctggtcg
    30421 cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa cagattattc
    30481 tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta cgcgaactaa
    30541 cgttactgcc gctcaacatt aaacatatgt cggtaaaggc atttttgcag acaggcgctc
    30601 cgcgtggcgc ggcactgatt attgctcctt ataccatgcc gttaccgctc ttttcaccac
    30661 cgctgatcta tacggacctg acactgacaa cacatcaaca ggagcagatc cgcaaaatgc
    30721 tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag cgccaccagc
    30781 gccatcaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc gaaaacggtc
    30841 atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa acggatgact
    30901 tcgctaccct ggcgagcggc aatatagcgc atggcggcca ggtggcagac cgtgaaggtg
    30961 ccgcaatgca gaatttgcac cactatcaac cacggcaacg ccgtagttgc tcccataatg
    31021 ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgtgc actacaacgg
    31081 cggaaaagtt tattactcag tgcaaagata atgacttccg ccaccacgcc cagcgaccac
    31141 aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg
    31201 taataggccg catgcgcccc ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg
    31261 cgaaccagcg ccaaccacgc tgaccaaccg gtgctctcct gttggcggct tgccccttgt
    31321 ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc caacgtcaac
    31381 agcacgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag cgccgagcca
    31441 atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg gaactgcttt
    31501 tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag
    31561 ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc gacagcaaag
    31621 agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgagaagg atcgctgacg
    31681 cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc caataacagg
    31741 ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa aggtagaaaa
    31801 atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa
    31861 accatgagtc cctcccgtca ggaggcgtta gtctggagtt aatcgaatgt tggtgcaagt
    31921 tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt cacaagcact
    31981 caaaaactgc gatccgtgac gcatgttgca aagataatgc gtaattatgc cgttacgctt
    32041 gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc
    32101 ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg aaaaacaacc
    32161 tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag ccccatgccc
    32221 accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg ctggctgttt
    32281 tcttgtgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc cggatcggta
    32341 ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg
    32401 tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata atcaatctcc
    32461 gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg aattttttcc
    32521 tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg tctgagacga
    32581 aacatcggta atacttttgg cagtaaattc acttccgccg atggcacaaa gccaatggtt
    32641 aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc cgcttgttcg
    32701 aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc
    32761 ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat ctggctgctt
    32821 aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag tgcctgcgcc
    32881 actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt atcgaaaaaa
    32941 cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc aacatcctta
    33001 tggcacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat atgaccacac
    33061 cctcagattt gaacatttac caactgattg acacccaaaa cggtcgggtc actccgcgta
    33121 tttataccga cccggacatt taccaactgg agcttgagcg tattttcggt cgttgctggc
    33181 tatttctcgc ccacgaaagc cagatcccaa aacccggtga tttctttaac acctacatgg
    33241 gagaagatgc agttgtcgtg gtgcgccaga aagacggcag catcaaggcg tttctcaacc
    33301 aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact cgcgccttta
    33361 cctgcccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat gtaccgctgg
    33421 aacctcgcgc ctacccacaa gggttgtgta aatcccactg ggggctaaac gaagttcctt
    33481 gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca ccgggcctgc
    33541 gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg
    33601 gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgcaactgg aaattcccgg
    33661 cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct gccgttcagg
    33721 tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa accgcccgcc
    33781 cggtgtggga aaccgccaaa gatgcgttgc aatttggtca ggacggtcac ggtagcggtt
    33841 tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt tcaagctatt
    33901 accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc ctgcgcctgg
    33961 cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc acgctccgcg
    34021 tctggcatcc gcgcggccct gatcaagtcg aagtgtgggc gttctgtatt actgacaaag
    34081 ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct tttggtcctg
    34141 ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa ttgcttaaag
    34201 gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc
    34261 gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc gctcgtggaa
    34321 tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa
    34381 ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag agttacatca
    34441 ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga aatttcgtga
    34501 ctggctggcg cagctcgacg aagagattcg ttacaccatg cgcaccacag ttaacgcgca
    34561 aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta atgacaccaa
    34621 agaccagctg gagcggcgaa tcgcccgtct ggaaacgggt atggcatggg cagaagagcc
    34681 gccgtcacgc acccgccact taatcagcaa ctgccaggta agcgaaaccg acatcccaaa
    34741 cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc gcgatgaaac
    34801 attctatgtc gggactcgtt ttgacaaagt tcgccgtctg gaagatgaca actggcgctt
    34861 gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc tgagtgtact
    34921 gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc
    34981 cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt ttatgccatt
    35041 aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga agatgacgcc
    35101 acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa agcgttatgc
    35161 ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg tgacattttc
    35221 atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt tttattaccg
    35281 gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcagg
    35341 ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga tttggcgaac
    35401 atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc gcggtcgatc
    35461 agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcgggc atctgggatc
    35521 acaatgcctc actggttaat actcccgcag agaggctgga aaccggcttc cacgagctgt
    35581 ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg gcgttaatcg
    35641 ccagtgaagg cagcatgatt ttcacactgt caatggcgga catcagctca tccacattga
    35701 ccggagcgtg aaactcctgc gagaacgtgc cgtgctgtaa taacaggatg cggtcacaca
    35761 ccagcggcaa ctcctccact tcactggaga taaacaccac gctttttcct tcggcagcca
    35821 gctcacggac aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgttggct
    35881 cgtcgagcaa caaaatctgg ctggcggcat agacccaacg accgatcact actttttgct
    35941 gattgccgcc agaaagcgtg ccgatgggcg tttcgctact ggcggcctta accgtcatcc
    36001 gctgcatcac ctcttcggtc aggcggcgga tggtggacca ttgcagcacg ccgttggcgc
    36061 taattttttg ccgattagtc agcactgtat tttcgtcaac gcccagccag ggaatgatcc
    36121 ccgcttcttt gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat
    36181 cggggtacgt gattttctcg ccgttgataa caatttcgcc ctgttcatac tcctccagcc
    36241 caacaatcgc tttcagcaat tcactgcgtc ctgcccccag caggccagca atgccgagca
    36301 cttcgccacg acgtagcgta aaactgatat cctccagctt gggcttatgg cgtaacgcac
    36361 ggacttccag cacggcctga tccacaattt cctgaggtgc aaccggcgca atatcaacgt
    36421 gatcgcgccc tagcattagc gacacaatat gatgcgtgga agtgttttcg agcatcacat
    36481 cgccagccac ctgcccgtcg cgcataacgg tggcacagga ggcaatgcgg cgaatttctt
    36541 ccatccggtg gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcacggcgc
    36601 ttatcaccag ttcaacttcc gcactggcaa gcgaactggt aggttcatca agaatgacca
    36661 cgcgcggttc gcctttcatc acccgcgcaa tttccaccag ctgcttttgc gccgggctta
    36721 gccctgaaac aagttgttca gggctaacgt caacgcccag cgcctgtaag caacgttggg
    36781 catcctgcgc catttgcagg taatcaatca tgccgttgcg gcggggccac tgaccgaggc
    36841 agaggttttc cgccaccgtc agcccttcca ccaaacttaa ctcctgataa accgcgcgta
    36901 cccccagttc agcggcacgg cgagtcaacg tagcttcgtc gccttccagt cgcgtctcac
    36961 caatccagat atcaccgcta tccggacgtt cgctaccggt aagcattcga atgagggtcg
    37021 atttgcccgc gccgtttttg cctaacagcg cacgaacctc gcctttattg agcgtgaagt
    37081 taacgttatc caacgcaacg acgccgggat aacgcttatt tcctgccacc acttttgcta
    37141 ccgggactgc ctctgttgcc gtgaacatag ggacctctgc gaatcagcga ttagggcaga
    37201 ccatcaacgt gcgttgccag ccactgttta ccgtcttccg ttttggtata gagatcgatt
    37261 ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg
    37321 aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg
    37381 ttttccagct cctgagcaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg
    37441 gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgattccccc
    37501 ataatggcgt tgagatccgg cgtggagata atcagttttt caccgacgga aatcgcttta
    37561 tctaaaacag tcccttcctg attagcgaca atttgcgcgc cgggaacgcg ggcttttaat
    37621 acttcttcaa accctttacg tcgttgcaca caaacttcaa aggcttcgca attgatgacg
    37681 gcaattttcg gctggtcaat tttgttggca ataaaataat cggcggcagc gttacccagt
    37741 tttttaccaa attccagcgg gtcacccacc agatatgccg agacatattt atcgacaccc
    37801 ttttgattaa tacaggtgtt gtagcaaatc accggaatac ccgcttcact ggcgcgacga
    37861 acggtacggc tactgccatt ttcagacact gccgataaaa taatagcatc gacattacgc
    37921 gccacgaggg tatcaacaaa ggtactttct ttcgaaatat caccctgggc gttagtttca
    37981 attaactgta cctgtactga agaatctttt gccgcatcct gaacgccctg acgcactcca
    38041 gcgtaatatc cctgagtatc aaggtatatt gcgccaatgg tcatttcttt atccgcagct
    38101 ctggcaaata gtgcgctacc taatagcgta gccattaata atagattacg ggttgttctc
    38161 atttttttag gcatagtgcc tcctgtaggt tttttattta caacggctta ttttaattat
    38221 ttgagcgtga gcggcagcgc gtgccgctca aatattacag ttaatagggt ttagtgaacg
    38281 tagttaaaaa taaaaggcat cacagtggtg aataatgccg ctttcggcca ggccggatta
    38341 atcgccgtca attcagcgcg agcagactca tattgcgcaa cctccccgag tcccgcggac
    38401 gccagcatcg ccaccatcag gcatttttct ttatgctgct gttgtaaatc gccatacagc
    38461 gacaacaggt caggttgtga aacagcaaag aaatctgcct caatactggt tttcgccatc
    38521 tcttgcgccc actgtttcat ttcactaaac agttgctgtg cgatttgttg ttcgcccagc
    38581 agtcgcagcg ccattccttg ccagaagaga taatcaaccg gctgatcgtt gtaataactg
    38641 tgaatgttaa tagtgcgatc gccggtcgcc gccagacgta aacaacgcgt cgcttcagtt
    38701 tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc agatgtcgtt atcagtttgc
    38761 cccggtaaac ggccttcgct taaattctcc ggataatgca gcgcggcatg aagcagttcg
    38821 ctggcctgct gcggctgtct ggcatcaaga tgctgccagg cgcgtaataa ctggttgagg
    38881 ataaactgac tggtgacctt cccttccccg ccttcccacg ggtggaattt gcgcgtggcg
    38941 agaatgtccg ccgctttgtc tgcctgcccc gtgagatgcc acaaattgag cagttctgcg
    39001 gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt ccagacgcgc cagtcgtttc
    39061 tccggtgtgg ctccacttaa cttatcaagc aaatcccgtt cgaaaagcag acgtgcatcc
    39121 tgcggcgcaa gctgataagc attatcaaga taacgcgcgg ccagctcata atcgtgttgc
    39181 ttattccacg catggatcgc taacccgcgc cagccgtcgg caaactccgg cgacatctct
    39241 acgcaacgtt gccataaggc aatggctttg ttgtagctac gtttgttgta gtagaagcag
    39301 gccagtaaat ggcgagcaaa ccagcactct tcaatactct ccagcgccgc cacttctttc
    39361 agcgtattcg ggaaacggac aaactgcggg aagacatcaa tggcttttgc gaccagttcg
    39421 ccacgttcgg ctttcggcag caagctggct tgcaggtaaa gcggcagcgt gcgctggcag
    39481 tccagtgcgt tcagcatctc tgccgccagg gtgggcattc cccagttaat cagttgcccg
    39541 gcggtcatca gggcgttaac gtcgcgtccc tgacacagac cgcgccactg cgcgagagct
    39601 gattcgctac gaccatcgaa ccagttcagc caccacagag tggcgttcag cggataatcg
    39661 cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac gaccacttaa caccagcagc
    39721 agattatgca ggcaaagcac ttcctgattg gttgggcagg cgcgaagact ttgttggcaa
    39781 aaatccagcc ccgcgtcgaa gttaccatta cgcgccgcca gtcgtgccag accataatag
    39841 cctccggctt tactgttgcc gctccagacc gcacgccaga aatcctcttc ggcttgttga
    39901 tattgtccct gacgttcgta agcactggcg cgaatcaaac tcgcctgtcc gcactgcgga
    39961 tttttgttca gcccatgtgc gcgtttcaga gcctgagtgg catacgccac cgcttgcggg
    40021 aaatctgcgc ggttatattc cagcatcgcc agcgccaggt tacagcggta atcaagcgga
    40081 tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac ggctggcatg atgatattgc
    40141 tccagatgct gaccgataaa ccaggcttca tctgtactgg taatgtcttg tgctgccagt
    40201 ggcgctttgg cgacgtccgg caacggcaac tcttgcggct gatgttcctg ataactcagt
    40261 acaatattgc cgtcggcatc ggagagctca atggtcagcc tttcaggatt gataccgtgc
    40321 aacacgccct ggatggcggt cgcaggcatc agtgccacgg catcatcaag taacgcgttg
    40381 catttgccga tttcgcggat cgccaggcgg tatccgttca acggagagat ggcatacagc
    40441 ccccactcaa tcccccgctc actacgctgg agtttgatta ccgcatcgcg ggaggcgttt
    40501 tgcaccatgc ccagagaatg gtaaggcagg aaatactgct caaaacgctt ctcttcgtaa
    40561 gcatcaagcc aggtaaaatc aggctggtta tcggcaaaaa taccggtcat cagttcgata
    40621 tacgggccgt tattatcagt taggctctta tcccacgcct ggccaaattc actgtgtccc
    40681 cagctccact gctttttacc tggcgcaata tggtggttgg caacgtgtag caaaccgcca
    40741 tcttcatcgt gacaccacgc gccaacaaaa tcgtactgcg atttttcggc catatatgag
    40801 gttggaacgg gcacattttt atagcgagaa atgtccactc cagcggagta gtccactttg
    40861 tagtaagtgc cggtggcgat ggggaaagcg gagacggccc gtttgccgtg atcaaacacc
    40921 gcagtcacat ccggcgggaa aacgctctga tgcccttccc cccctttcac tgccgggttg
    40981 gcccaccaca agaaatgacg cggcgtggcg ttgccgttat agacgcggct ggcgatttcc
    41041 agcgccgccc ggtcagggcg cagggtgaaa cctgtcatca cctgtaaacc atgcatcggc
    41101 tccgtttcgc cgacccacac cgtctgtgca ccgtcttcat gggcttcgag ggtgaaatca
    41161 acgggcataa aggtggtcgg gcgatggtgt tgcggccagt taaactcaat cccgccagag
    41221 atccacggtc ccagcagccc caccagcgca ggtttaatga cttcattgtg atagacaaaa
    41281 tcgcgctgtt tcactttatc ccatgcgcga tgcacccgac cgcccagttc cggcagtatc
    41341 atcactttga tgtagtcgtt ttccagccac accgcctgcc aggatttcag ggttttctgt
    41401 tcactcagcg tatcggtcac gccgtaggga taaaccgcgc cggacgatcc ctgataaacg
    41461 cgattttcca ggaacatggg atgtatatcc tgcggcccgg tttcataggt cgggatctca
    41521 acgcgctctt gccacacttt tactggagtc atggtgccct caatattaac aagacatact
    41581 gaattaaaag atttgtggca gtgtattgaa caatctggca atgttttcgc ggaataatca
    41641 cgcaattaac taaacaaggt ttagtgaaga tgagagcctg cattaacaat caacagattc
    41701 gccaccataa caaatgcgtg attctggaac tgctgtaccg gcaaaagcgc gccaataaat
    41761 caacgctggc ccggctggcg caaatttcga ttccggcagt cagtaatatt ttgcaggaac
    41821 tggaaagcga aaaacgggtg gtgaatatcg acgatgaaag ccagacgcgc gggcatagta
    41881 gcggtacatg gctgattgcg ccggaaggtg actggacgct gtgtctgaac gtgacgccca
    41941 ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc gaaaggtgaa tttgagtatt
    42001 tacagattga tgcaccgacg ccgcaggcgc tgctggccga aatcgaaaaa tgctggcatc
    42061 gccaccgtaa attgtggccg gaccgcacca tcaatctggc gctggcaatc cacggtcagg
    42121 ttgatccagt gacaggagtg tcgcaaacca tgccgcaagc gccgtggaca acgccggttg
    42181 aggtgaagta tctgctggaa gagaagctgg gcattcgggt gatggtcgat aatgactgcg
    42241 tgatgctggc gctggcggaa aaatggcaaa ataattcgca ggaacgggat ttctgcgtga
    42301 tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa cgagcaaatt tatcgcggca
    42361 gtttgtatgg tagcggacag attggtcaca ccatcgttaa tccggatggc gtcgtctgcg
    42421 actgtggacg ttacggctgc ctggaaaccg tcgcctcgtt aagtgcttta aaaaaacagg
    42481 cgcgggtatg gctaaaatca caaccggtta atactcagct tgatcctgaa aaactgacaa
    42541 ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg gatcaccagc tgggttgacc
    42601 gcagtgccaa tgccattggt ttgagtctgt ataacttcct caacatcctc aatattaatc
    42661 agatttggtt gtacggtcgc agttgtgcct ttggtgagaa ctggcttaat accattattc
    42721 gccagacggg atttaacccg ttcgaccgcg acgaaggacc gagcgtgaaa gcgacgcaaa
    42781 ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg aattggctat ttgtatgttg
    42841 aggcgcagtt acgacagatt tgatggcacg ataaggtaga aaggcttccc gaaggaagcc
    42901 ttgatgatca taaacgaaaa attgcctgat gcgctacgct tatcaggcct acacggagat
    42961 tgcaatatat tgaatttgcg agattttgta ggcctgagaa ggtgttcatg ccgcatccaa
    43021 catgaacaac gggcacattg tcagcaaatc accgtttcgc ttatgcgtaa accgggtaac
    43081 gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca tcattgatgc
    43141 tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct ttgaagccgc
    43201 gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg ctcttcggat
    43261 cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg gcgtctgctt
    43321 ctttaccggt caggttttta tcgaccagat caaccaggaa cagatggtta tcagtaccgc
    43381 cggaaaccac tttgtagccg cgttctagga aaacttctac catcgcttta gcgtttttag
    43441 cgacccgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga gcaaccgctt
    43501 taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg gcagagttca
    43561 gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg cgcggacccg
    43621 ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg ttcgggtaga
    43681 cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa gcgccgatgc
    43741 tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca gagaagccac
    43801 cgataatcat tttcggtttg tgttctttgg cttgtttttc cagatcggcg tagtcgatat
    43861 gaccggtagc atcaataccg taaggaacga tgttgtacag tttaccggag aagttaaccg
    43921 gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg gtatcacctg
    43981 gttccagcag cgcggtgtac accgcaaagt tagcctggga gccggagtgc ggctggacgt
    44041 tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt tcaacgatat
    44101 caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca tatttgttgg
    44161 tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg gaggcgatca
    44221 gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac agttcggcat
    44281 cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc agctaacaat
    44341 aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat aaccgaatca
    44401 ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc aggctacgca
    44461 aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg tttgtgatgc
    44521 aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt acattgcagg
    44581 gctatttttt ataagatgca tttgagatac atcaattaag atacaaaaaa aggaagacca
    44641 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
    44701 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
    44761 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
    44821 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
    44881 aatcgcgcag aagcacacca gtttccagat caaaccggaa cagtacaaca tcgtcggtga
    44941 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
    45001 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
    45061 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
    45121 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
    45181 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
    45241 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtattgc
    45301 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
    45361 cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg cagatgacac
    45421 accagtgacg ttaatctctg ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
    45481 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
    45541 tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc gctttaccgc
    45601 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
    45661 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
    45721 tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa cagttagtgg atctgggcgt
    45781 gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt gatttgatgt
    45841 tgccggatgg aaacatccgg caacccctga cgcggtttaa attgccgcgt cgtcctcttc
    45901 accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac cgtcaccgat
    45961 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
    46021 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
    46081 cagctcggta tggcctttct ggcggccaaa acctttcacc tcagtgaccg tcatgccggt
    46141 aatgccgact tcagccagtg cttcgcggac atcgtccagc ttgaagggtt taataatcgc
    46201 atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct gctaaacgta
    46261 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
    46321 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
    46381 ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg cctcaacaaa
    46441 ggttggcagc gccgtatttt cgccctccag cgcctgctct actaatgcat cactaatcac
    46501 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
    46561 cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc ggacaaacgg
    46621 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt
    46681 gcgttccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
    46741 acggaattcc ccgcgcgcca tcgcttttgg caggtcgcgg tgagtggcag aaataatccg
    46801 cacgtcgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
    46861 gcgcagcagt ttgacctgca acggtgcggg catatctcca atctcatcga gaaatagcgt
    46921 accgccttcc gccgcctgga ataaaccttc gcgattgctg acagcaccag taaacgcgcc
    46981 acgcgcatga ccaaacagct ccgactccag caattgctca ggcaatgcac cacagttaat
    47041 agcaataaat ggtttgctat tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
    47101 tttcccggtg ccgctctgac cgttaatcaa aacgctaacg tctgattgcg ccaccagccg
    47161 cgtctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaatcgcct cgcgccagcg
    47221 ttcgtcggtg gctggcgcgg attgttccag cgcatcatca attgcctgat atagcgcgtc
    47281 tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
    47341 aggaatagaa ccatgcgcgg taagaataat taccggcatt cccggctgca ctttctggat
    47401 ttcagcaaac agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
    47461 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
    47521 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
    47581 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
    47641 gcttttcacc ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
    47701 gctggcgttc aatatcagtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
    47761 gtagaacgtg atgttgctgg cgcagagcgt ccagctcgct gtcgctagac tgctggagtt
    47821 tactgtagcg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
    47881 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
    47941 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
    48001 aggtattttg ccagctgccg tcgtcatatt ggcgggcttg ctgacgcgac tgtgcaggca
    48061 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcagttgatt
    48121 taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc agttgataaa
    48181 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgactc tgcacgcaac
    48241 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
    48301 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
    48361 agtctggaca tggacagcgc ggagaaaccg gagcgtacat gtcgtacgtg aggatttcga
    48421 gcactgcccg gggccgaact gacaaataaa atagcctgat gggatagact gttagcatta
    48481 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    48541 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    48601 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    48661 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    48721 cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    48781 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    48841 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    48901 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    48961 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc ggctgctgct
    49021 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc
    49081 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    49141 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    49201 acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat tgcccagaga
    49261 acgcccttcc cccaaccgat tgatcatccg ttcaatattt ttcaccggcc cgataatcat
    49321 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    49381 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    49441 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    49501 ggcggcagca tcgggaccac tgttgttgca ctgaagttgg gccagattgt tcaagtcctg
    49561 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    49621 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcat ccagcacgca
    49681 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcattgctt cgctgcgccg
    49741 ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg tcgctaagcg cattcaggct
    49801 ttgccaggct tgccatgcca gcaccaacag gggcagcaga atcagcaaaa atgccagcat
    49861 taccagttgt cgtaatgagc ggggaaaaac ggggcagcgt ttcaaggtgt tactctcgtc
    49921 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    49981 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    50041 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    50101 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgat tactattatg
    50161 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    50221 gggtttatgg cttccccgct atttgaatgt aaatcagcca ctgtgtctct aaaaagagac
    50281 aacttaagaa aaacttatta gattacaata aaatcaatca tttaagtgtc gccagaaagc
    50341 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc gaagttggga
    50401 gcttataata gcggttgtat ttaatgccgg atgcggcgtg aatgcctcat ccgggctgca
    50461 aaaccaatga gtttgacggc ttaccccaac tgcttacgtg cattgcggaa aatgcgcatc
    50521 catgggccat cctcgcccca gttttcagga tgccaggagt tgctgacagt acggaaaaca
    50581 cgttccgggt gcggcatcat aatggtgact cgaccgcttt cagtcgtgac cgccgtaata
    50641 ccgttcggag aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg
    50701 acatagcgca gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc
    50761 acttccacgc gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc
    50821 tgcaacagca gcgacgggct ttgggtcact tcaaccaggc tgaaacgcgc ttcaaagcga
    50881 tcggaggtat tgcgcacaaa acgtggccac aactcactac ctgggatcag ttcacgcaga
    50941 ttagacatca tctggcaacc gttacatacc cccagcgcca gcgtttgcgg acggtggaag
    51001 aaggttgcaa actcatcgcg tacacggtca ttgaacagga tagacttcgc ccaaccttca
    51061 ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg cgaccagggc gtggaaatcc
    51121 tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca cgtcgatagc atcgaagcct
    51181 gcacggtgga aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagcaca
    51241 gcaactttcg gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg
    51301 aacgacagtt ttacattcag gcccggatcg gcgtcgtttg atttcgcctg atgctcctga
    51361 tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag
    51421 acacgcaacg tagtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa
    51481 cggtcaccgg aaaccgcctg cccaacataa tggacgcaat cagcaagccc atgctgtgcc
    51541 agtacggact cgaccgcttc acggtcagcg gcacgaacct gaatgaccgc acccagttct
    51601 tcgttaaaca acgccgccag gcgatcgtca cccagagtgg cgatatccgc gtaaatgccg
    51661 caatgaccag caaaggccat ttccgccagc gttaccagca gaccgccatc agagcggtcg
    51721 tgataagcca gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc
    51781 agttgcgcga catcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc
    51841 agcgccgttg cgcccagcgc gttattgcct ttgcccaaat caatcagcag cagtgcgtta
    51901 tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat cttccacgcg ggcaaatgca
    51961 gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg
    52021 gttttcatcg acatagagtc tttacccacc gggatcgtca ggcccagcgc cggacaaagc
    52081 tcttcgccca cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc
    52141 gccgccatcc agttggcgga aagtttaatg cgtttgatat cgccaatctg tgttgcggca
    52201 atattggtta acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca
    52261 accggcgcac gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta
    52321 gtgaccgcgc agttagcgac cggcacctgc cacggcccca ccatctgatc gcgcgccacc
    52381 atgccggtta cgctgcggtc accgatggtc accaggaaag ttttctccgc cacggtcggc
    52441 agatgcagta cgcgtttcac cgcgtctgca atggtgatcc cttcacggac cagcgcatcg
    52501 cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg
    52561 tccagcggca gatcgatcgg ctgattatca aaatgacgat cgtccagaga aagatgcagt
    52621 tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca cagttcgtca
    52681 aacagcggta actgatcggc agcaaccgcc agcacgtagc gttcctggga ttcgttacac
    52741 cagatttcca gcgggctcat gcccggttcg tcgctcagga tatcgcgcag ttcaaattta
    52801 ccgccgcgcc cgccgtcgct taccagttcc ggcatcgcgt tagaaagacc gcctgcacca
    52861 acgtcgtgga taaacaggat tgggttggca tcaccaagct gccagcaacg atcgatcact
    52921 tcctggcagc gacgctccat ttccgggttg tcgcgctgga cggaagcaaa gtcgaggtcg
    52981 gcatcagact gacctgacgc catggaagac gccgctccgc cgccaagacc gatgttcatt
    53041 gccgggccgc cgagaacgac cagcttcgca ccgacgttga tctcgccttt ttgtacgtga
    53101 tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc
    53161 tcttcgccgt tgtggctgtt cactttttct tcataagtac ggaagtagcc attcagtgcc
    53221 ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg ggccttcggt catgatgtcc
    53281 agcgcggtga caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagccc
    53341 ggaatacgca ggttggaaac ggagaaaccg accagaccag ctttcggctt cgcgccacgc
    53401 ccggtggcgc cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacggt
    53461 gaaatcgccg tcgggtggtt gtgggtttca actttcatca ggatatgcgc cggttcctga
    53521 tggaaatcgt agcggcccgt ttcgtggtcg gcaaaataac ggcccacttc agaaccttcc
    53581 attacggcgg cgttatcttt ataagcagag agaacgtgat ctggcgtggt ttcgaaagta
    53641 tttttgatca tcctgaacag cgatttcggc tgctgttcac cgtcgataat ccagtcggca
    53701 ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag cgaacatata cagctcgatg
    53761 tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc
    53821 gccagagcca ggccaagacg caggttagcg tcgatcagcg cctgacggcc ctgccccagc
    53881 aaatcaacgc tggtaaccgg agtcggttga tggtgggcaa acaactgctc tgcatcatct
    53941 aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat
    54001 tgttcattgg tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt
    54061 acctgttgta gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg
    54121 gtgccaggac gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc
    54181 gggccatatt ttagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg
    54241 aggtcagcaa aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga
    54301 aaacgtgcca gcagtttgtt gattcggaat gccgacaatg caggcgaacc acgcagaatt
    54361 tccatcataa gtctctcgtc ttctaagctt tcggtgtacc caaggggggg aaacgggcgt
    54421 cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt
    54481 gaattagcaa cgcgtgccgc caatggctgt aataagttgc catctggcgc aggtttacgc
    54541 aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc aagctgggac
    54601 gc
//
LOCUS       DAZUGK010000008        52114 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZUGK010000008 DAZUGK010000000
VERSION     DAZUGK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.008"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..105
                     /locus_tag="ACHY5W_000398"
     tRNA            30..105
                     /locus_tag="ACHY5W_000398"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Val,seq:tac)
     gene            110..185
                     /locus_tag="ACHY5W_000399"
     tRNA            110..185
                     /locus_tag="ACHY5W_000399"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Lys,seq:ttt)
     gene            305..646
                     /locus_tag="ACHY5W_000400"
     CDS             305..646
                     /locus_tag="ACHY5W_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFS4445364.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(637..1563)
                     /locus_tag="ACHY5W_000401"
     CDS             complement(637..1563)
                     /locus_tag="ACHY5W_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4445365.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1653..2651
                     /locus_tag="ACHY5W_000402"
     CDS             1653..2651
                     /locus_tag="ACHY5W_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFS4445366.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(2648..2866)
                     /locus_tag="ACHY5W_000403"
     CDS             complement(2648..2866)
                     /locus_tag="ACHY5W_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFS4445367.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFTRKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2868..4883)
                     /gene="ligA"
                     /locus_tag="ACHY5W_000404"
     CDS             complement(2868..4883)
                     /gene="ligA"
                     /locus_tag="ACHY5W_000404"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4445368.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4954..5952)
                     /gene="zipA"
                     /locus_tag="ACHY5W_000405"
     CDS             complement(4954..5952)
                     /gene="zipA"
                     /locus_tag="ACHY5W_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFS4445369.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHST
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLRENQDIIREVKDANA"
     gene            6182..6943
                     /gene="cysZ"
                     /locus_tag="ACHY5W_000406"
     CDS             6182..6943
                     /gene="cysZ"
                     /locus_tag="ACHY5W_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFS4445370.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAICGATAMWVDCYRDKHAMWR"
     gene            7128..8099
                     /gene="cysK"
                     /locus_tag="ACHY5W_000407"
     CDS             7128..8099
                     /gene="cysK"
                     /locus_tag="ACHY5W_000407"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4445371.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8483..8740
                     /gene="ptsH"
                     /locus_tag="ACHY5W_000408"
     CDS             8483..8740
                     /gene="ptsH"
                     /locus_tag="ACHY5W_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFS4445372.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8785..10512
                     /gene="ptsI"
                     /locus_tag="ACHY5W_000409"
     CDS             8785..10512
                     /gene="ptsI"
                     /locus_tag="ACHY5W_000409"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFS4445373.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10553..11062
                     /gene="crr"
                     /locus_tag="ACHY5W_000410"
     CDS             10553..11062
                     /gene="crr"
                     /locus_tag="ACHY5W_000410"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4445374.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11105..11956)
                     /gene="pdxK"
                     /locus_tag="ACHY5W_000411"
                     /gene_synonym="thiJ"
     CDS             complement(11105..11956)
                     /gene="pdxK"
                     /locus_tag="ACHY5W_000411"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFS4445375.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12061..12429
                     /locus_tag="ACHY5W_000412"
     CDS             12061..12429
                     /locus_tag="ACHY5W_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFS4445376.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12432..13343)
                     /gene="cysM"
                     /locus_tag="ACHY5W_000413"
     CDS             complement(12432..13343)
                     /gene="cysM"
                     /locus_tag="ACHY5W_000413"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFS4445377.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13477..14574)
                     /gene="cysA"
                     /locus_tag="ACHY5W_000414"
     CDS             complement(13477..14574)
                     /gene="cysA"
                     /locus_tag="ACHY5W_000414"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFS4445378.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14564..15439)
                     /gene="cysW"
                     /locus_tag="ACHY5W_000415"
     CDS             complement(14564..15439)
                     /gene="cysW"
                     /locus_tag="ACHY5W_000415"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFS4445379.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15439..16272)
                     /gene="cysT"
                     /locus_tag="ACHY5W_000416"
     CDS             complement(15439..16272)
                     /gene="cysT"
                     /locus_tag="ACHY5W_000416"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFS4445380.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16272..17288)
                     /gene="cysP"
                     /locus_tag="ACHY5W_000417"
     CDS             complement(16272..17288)
                     /gene="cysP"
                     /locus_tag="ACHY5W_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFS4445381.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALT
                     PPFEQQWAKDNGGDRLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17446..18237)
                     /gene="ucpA"
                     /locus_tag="ACHY5W_000418"
     CDS             complement(17446..18237)
                     /gene="ucpA"
                     /locus_tag="ACHY5W_000418"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFS4445382.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVVDPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18366..19223)
                     /gene="murR"
                     /locus_tag="ACHY5W_000419"
     CDS             complement(18366..19223)
                     /gene="murR"
                     /locus_tag="ACHY5W_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFS4445383.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAPFVQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19387..20283
                     /gene="murQ"
                     /locus_tag="ACHY5W_000420"
     CDS             19387..20283
                     /gene="murQ"
                     /locus_tag="ACHY5W_000420"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4445384.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLNQHGGFIRKALEKE"
     gene            20287..21711
                     /gene="murP"
                     /locus_tag="ACHY5W_000421"
     CDS             20287..21711
                     /gene="murP"
                     /locus_tag="ACHY5W_000421"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFS4445385.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21716..23020
                     /gene="pbp4b"
                     /locus_tag="ACHY5W_000422"
     CDS             21716..23020
                     /gene="pbp4b"
                     /locus_tag="ACHY5W_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFS4445386.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGMAKKYDGSVLMAQPVKATTDTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(23078..23977)
                     /gene="yfeX"
                     /locus_tag="ACHY5W_000423"
     CDS             complement(23078..23977)
                     /gene="yfeX"
                     /locus_tag="ACHY5W_000423"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFS4445387.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24073..24648)
                     /locus_tag="ACHY5W_000424"
     CDS             complement(24073..24648)
                     /locus_tag="ACHY5W_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFS4445388.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24709..25158)
                     /locus_tag="ACHY5W_000425"
     CDS             complement(24709..25158)
                     /locus_tag="ACHY5W_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFS4445389.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(25145..25570)
                     /locus_tag="ACHY5W_000426"
     CDS             complement(25145..25570)
                     /locus_tag="ACHY5W_000426"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFS4445390.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25784..26653
                     /gene="amiA"
                     /locus_tag="ACHY5W_000427"
     CDS             25784..26653
                     /gene="amiA"
                     /locus_tag="ACHY5W_000427"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFS4445391.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGSAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26657..27556
                     /gene="hemF"
                     /locus_tag="ACHY5W_000428"
     CDS             26657..27556
                     /gene="hemF"
                     /locus_tag="ACHY5W_000428"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFS4445392.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27540..28613)
                     /gene="eutR"
                     /locus_tag="ACHY5W_000429"
     CDS             complement(27540..28613)
                     /gene="eutR"
                     /locus_tag="ACHY5W_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFS4445393.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGEGVTPNP"
     gene            complement(28659..29159)
                     /gene="eutK"
                     /locus_tag="ACHY5W_000430"
     CDS             complement(28659..29159)
                     /gene="eutK"
                     /locus_tag="ACHY5W_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFS4445394.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29172..29831)
                     /gene="eutL"
                     /locus_tag="ACHY5W_000431"
     CDS             complement(29172..29831)
                     /gene="eutL"
                     /locus_tag="ACHY5W_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFS4445395.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29841..30728)
                     /gene="eutC"
                     /locus_tag="ACHY5W_000432"
     CDS             complement(29841..30728)
                     /gene="eutC"
                     /locus_tag="ACHY5W_000432"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFS4445396.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30749..32110)
                     /gene="eutB"
                     /locus_tag="ACHY5W_000433"
     CDS             complement(30749..32110)
                     /gene="eutB"
                     /locus_tag="ACHY5W_000433"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFS4445397.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(32122..33525)
                     /gene="eutA"
                     /locus_tag="ACHY5W_000434"
     CDS             complement(32122..33525)
                     /gene="eutA"
                     /locus_tag="ACHY5W_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFS4445398.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIEPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(33522..34748)
                     /gene="eutH"
                     /locus_tag="ACHY5W_000435"
     CDS             complement(33522..34748)
                     /gene="eutH"
                     /locus_tag="ACHY5W_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFS4445399.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(34865..36052)
                     /gene="eutG"
                     /locus_tag="ACHY5W_000436"
     CDS             complement(34865..36052)
                     /gene="eutG"
                     /locus_tag="ACHY5W_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFS4445400.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(36042..36878)
                     /gene="eutJ"
                     /locus_tag="ACHY5W_000437"
     CDS             complement(36042..36878)
                     /gene="eutJ"
                     /locus_tag="ACHY5W_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFS4445401.1"
                     /translation="MAHDEQWLTPRLQTAATRCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(36889..38292)
                     /locus_tag="ACHY5W_000438"
     CDS             complement(36889..38292)
                     /locus_tag="ACHY5W_000438"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFS4445402.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(38304..38591)
                     /gene="eutN"
                     /locus_tag="ACHY5W_000439"
     CDS             complement(38304..38591)
                     /gene="eutN"
                     /locus_tag="ACHY5W_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFS4445403.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(38698..38991)
                     /gene="eutM"
                     /locus_tag="ACHY5W_000440"
     CDS             complement(38698..38991)
                     /gene="eutM"
                     /locus_tag="ACHY5W_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFS4445404.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(39030..40046)
                     /gene="pta"
                     /locus_tag="ACHY5W_000441"
     CDS             complement(39030..40046)
                     /gene="pta"
                     /locus_tag="ACHY5W_000441"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4445405.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(40043..40846)
                     /gene="eutT"
                     /locus_tag="ACHY5W_000442"
     CDS             complement(40043..40846)
                     /gene="eutT"
                     /locus_tag="ACHY5W_000442"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFS4445406.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCHQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMLLSVTKQPLTVKQIQQRLGETQ"
     gene            complement(40843..41544)
                     /gene="eutQ"
                     /locus_tag="ACHY5W_000443"
     CDS             complement(40843..41544)
                     /gene="eutQ"
                     /locus_tag="ACHY5W_000443"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFS4445407.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(41519..41998)
                     /gene="eutP"
                     /locus_tag="ACHY5W_000444"
     CDS             complement(41519..41998)
                     /gene="eutP"
                     /locus_tag="ACHY5W_000444"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFS4445408.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(42011..42346)
                     /gene="eutS"
                     /locus_tag="ACHY5W_000445"
     CDS             complement(42011..42346)
                     /gene="eutS"
                     /locus_tag="ACHY5W_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFS4445409.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGLLLN
                     YTLCEMTKS"
     gene            complement(42640..44919)
                     /gene="maeB"
                     /locus_tag="ACHY5W_000446"
     CDS             complement(42640..44919)
                     /gene="maeB"
                     /locus_tag="ACHY5W_000446"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFS4445410.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALQPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKSGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQVLELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            45208..46158
                     /gene="tal"
                     /locus_tag="ACHY5W_000447"
     CDS             45208..46158
                     /gene="tal"
                     /locus_tag="ACHY5W_000447"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS4445411.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            46178..48181
                     /gene="tkt"
                     /locus_tag="ACHY5W_000448"
     CDS             46178..48181
                     /gene="tkt"
                     /locus_tag="ACHY5W_000448"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4445412.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFIRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPILPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(48277..49320)
                     /locus_tag="ACHY5W_000449"
     CDS             complement(48277..49320)
                     /locus_tag="ACHY5W_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFS4445413.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPTQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITEFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     DTNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(49446..50021)
                     /gene="nudK"
                     /locus_tag="ACHY5W_000450"
     CDS             complement(49446..50021)
                     /gene="nudK"
                     /locus_tag="ACHY5W_000450"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFS4445414.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(50089..52068)
                     /gene="aegA"
                     /locus_tag="ACHY5W_000451"
     CDS             complement(50089..52068)
                     /gene="aegA"
                     /locus_tag="ACHY5W_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFS4445415.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
BASE COUNT        12096 a        14541 c        13329 g        12148 t
ORIGIN      
        1 tttcttgtaa aaatgtgaag taccgaagtg ggtgattagc tcagctggga gagcacctcc
       61 cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc
      121 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
      181 caccaatgta aaaaggcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga
      241 tttttcttct caagttcctt gttcttctgc cgatattacc tcgtacagtg acgaggactg
      301 aacaatggcc actatctctt caacttcaat tccttccata caaacacaga gtagtaacag
      361 ggcatcacaa ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa
      421 actcactcaa caaattaaag agattgtaga cactagcggc agtgctgaag acaaacaaaa
      481 acaggccgaa ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca
      541 gagacagcag gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct
      601 tctaaatcct gttgaaaata cgactcatat cgatatctat atctgataca acggatcccc
      661 ttcccgacca gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc
      721 ggcggtggaa agtgaacggt tcttcctgcg caccagcatc aactgccgtt caacaaccgg
      781 cgtaatgcgc tttaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcgggaag
      841 aatactgatg ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc
      901 ctgcacaata ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct
      961 gcctgacgcg taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata
     1021 atcttccacg gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat
     1081 cgcttcacat tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc
     1141 tccctggcga atactttcca tcacccactg ctgcggacga tcgtgcagga caaactgaat
     1201 atctggatag cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt
     1261 ctggctggca gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg
     1321 caacgtgctg ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc
     1381 atctgtaagc accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc
     1441 cagctccttc acactgtgac ttactgccga ctggctcagg ccgatacgct ctcctgcacg
     1501 actaaaactt ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt
     1561 catctgtttt cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag
     1621 cacaatagaa ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt
     1681 tcaccttaac cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt
     1741 tcgtcccctt ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg
     1801 gcgcgaagtt gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt
     1861 gggtaatgtg cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga
     1921 aaccggtaaa tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg
     1981 ctaccgtgca gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg
     2041 tctgttctgc gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc
     2101 tggtgatgaa tgttcacggt gcagggggca gccttgagca ggtcggtaaa attatgctgc
     2161 aactgctgct gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt
     2221 cgcgcaataa aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata
     2281 cagcgttcag cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc
     2341 tgtttatcgt ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta
     2401 tggcacgccg actaggcttc aataaagcag atgaaattac tatcgtcttt tgtggttcga
     2461 aaaagagtct ggcaaatggc atcccgatgg caaacattct tttccccaca tcggtgatcg
     2521 gtatgatggt gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc
     2581 tggcgcgtcg atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcaccg
     2641 ataaagctta aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa
     2701 tcagcagcgt gatttgcatt agctcaccga gcttccctgc cggaaactca tctttgcggg
     2761 taaaccacag cagatactcc tccggcaggt cgattaagcg acgccctttg tatttgccaa
     2821 acggcattat cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc
     2881 aaacgcagca tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt
     2941 ttagatcctg cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg
     3001 actttcgcgc ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag
     3061 ctgcccgtaa gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata
     3121 acgatcggcg caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg
     3181 ctttcttcgg caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc
     3241 ttttgcagct cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc
     3301 agacctgctg cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca
     3361 aaggtggttt ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccga ttttggcccc
     3421 atacgctcca gtccggtaag tttgcctgcg gtgagtttga acagatccgc cggagtgtgg
     3481 acatattctt tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc
     3541 gcacggcggg aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca
     3601 ccggtacagc gggcaacagc ttcaccttcc acacgctcaa cgtcagaacc acataccgga
     3661 caatgcgtcg ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta
     3721 accacctgcg ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa
     3781 ccaagacgtt cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgcctgca
     3841 acatggacag gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac
     3901 tcgacgtcac gcacaaaggt catctgttcc tgcgccggga atttaaacgc taccgcccag
     3961 cgcggggcac gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc
     4021 accacgccgt cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgataaaac
     4081 gccagcactt cttccgccga ttcacaaagc gttacccgat cgctgaccgg caacccccac
     4141 tgtttaaatt gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca
     4201 ccaacgccat agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga
     4261 cgcagtgaac cagctgccgc attacgtggg ttagcaaaca ctttcccgcc cgtgcgtcgc
     4321 gcatcttcgt taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc
     4381 agacgcgccg ggatattctc tccgtgcagc ttcagtggaa tggcgcgaat ggtacgcacg
     4441 ttagacgtga tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg
     4501 ccattttcat acagaatact gacagcaagg ccatccagct tcagctcaca gcaccaggtg
     4561 actttctcgt tgcttttcag acggtcctgc actcgtttgt tgaaggcaag aaagctttct
     4621 tcatcaaaaa cgttatccag cgacagcatt ggcacttcat ggcgaatctg gctgaaagcc
     4681 gccagcggcg cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt
     4741 ttggtttcca gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc
     4801 ggcgcatcca tcacatgata aagatattca tgatggcgaa gcgtcgttcg cagttctgtc
     4861 agttgttgtt cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg
     4921 ggttcgaaga ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg
     4981 gatgatgtcc tggttctcac gcaatttctg cggagtcatc atacggcgct gatcgtcaag
     5041 cacgacaccg cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt
     5101 ctgcagctcg tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa
     5161 atccttcatt tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc
     5221 cgggccgctg ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa
     5281 gcccgcttgt tgaatgctgt taagaagcag ttcaccattt agctcgctac cgtgatgcgc
     5341 cgcgacgttc atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg
     5401 ttccgctaca ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg
     5461 tgccggttgc ggtgttgaat gcactggctg cggcgctggc gcgacctgtg gcgaaactgg
     5521 ctgctgcaac ggctgttcag gctgcggctg ataggcaggc tgctgcacgg gctgctgcac
     5581 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggac
     5641 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
     5701 agcagcctca tgctcctggg cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
     5761 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
     5821 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
     5881 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
     5941 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
     6001 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
     6061 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
     6121 aattacccgg taaaatacgc acaattttcc tgggattggt caaaaggagc tcatcctgat
     6181 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
     6241 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
     6301 tttgttgatg gggggcgcat tctggtggct ctttacgcaa ctcgatgtct ggatcccgac
     6361 tctcatgagt tatgttccgg actggttaca atggctgagc tatctgttgt ggcctctggc
     6421 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
     6481 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
     6541 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
     6601 gcaaaaattt gcctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
     6661 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
     6721 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
     6781 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gtctgtttac
     6841 gatgatcccg ctgcttaatc tgttcattat gcccgttgcc atttgtggcg cgacggcgat
     6901 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
     6961 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
     7021 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
     7081 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
     7141 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
     7201 acggacgcat tcttgcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
     7261 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
     7321 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
     7381 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
     7441 aagcgttagg tgcaaacctg gtgctgaccg aaggtgctaa aggcatgaaa ggcgcaatcc
     7501 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
     7561 gcaatccggc aaaccctgaa attcacgaaa agaccaccgg tccggagatc tgggaagata
     7621 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
     7681 tcagccgcta cattaaaggc accaaaggaa agaccgatct tatctctgtc gccgttgagc
     7741 caaccgattc tccagttatc gcccaggcgc tggcaggtga agagattaaa cctggcccgc
     7801 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
     7861 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
     7921 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcggcg ttgaaactac
     7981 aagaagatga aagctttacc aacaagaata ttgtggttat tctaccatca tcgggtgagc
     8041 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
     8101 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
     8161 caaactttcg ccccacctgg cattgattca gcctgtcgga actggtattt aaccagacta
     8221 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
     8281 atttggttca attcttcctt tagcggcata atgtttaatg acgtacgaaa cgtcagcggt
     8341 caacatccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
     8401 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
     8461 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
     8521 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
     8581 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
     8641 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
     8701 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
     8761 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
     8821 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
     8881 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
     8941 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
     9001 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
     9061 attaaagata agcacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
     9121 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
     9181 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
     9241 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
     9301 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
     9361 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
     9421 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
     9481 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
     9541 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
     9601 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
     9661 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
     9721 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
     9781 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
     9841 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
     9901 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
     9961 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    10021 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    10081 ccggctgccg caacaattgc gcgtcattta gccaaagaag ttgatttctt tagtatcggc
    10141 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    10201 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    10261 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    10321 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    10381 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    10441 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    10501 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    10561 gttcgataaa ctgaaatctc tggtttccga cgacaagaag gataccggaa ctattgagat
    10621 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    10681 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    10741 tgtagacggt accattggta aaatctttga aaccaaccac gcattctcta tcgaatctga
    10801 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    10861 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    10921 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    10981 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    11041 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca tcggcgccat
    11101 ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc tgctgggtgt
    11161 agcgcatcac ttccagtacg cgcaaccccg ctcggtgcac tgcatcggtt aacgcctttc
    11221 ctttcagcaa accactgata agctgagcac aaaacaggtc gccagtccct ttcaggtcgg
    11281 tttttacccg tgaatgggaa atgacattca cgctgtcggc agtgaccacc accacctgca
    11341 tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt aatgtgtctg
    11401 aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt ttaccggtca
    11461 ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt aaatattgtc
    11521 gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg ccaatcaccg
    11581 gatcgaccat gatcaatagg tcaggatggt ctttgcgtag cgcagtcagc cactcggcaa
    11641 ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagcacga agttggcgca
    11701 gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcgtcc ggaatcgcac
    11761 caccgtagaa agtgtcataa tgcggcgtat tgctcagcaa taccgtcggc acggcaaaga
    11821 cattcaggcc gttctgtttg atagcaggca cggcaatgct gttgcccacg ctgccgtaaa
    11881 ccacctgcga ctgcacggcg acgatatccg cctgcagcgc cctgctctta tcgttaaaca
    11941 acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt attccaaccg
    12001 aaactttacc tgattctggc agtcaaatcg gctattacaa aacaaggata aggtaattca
    12061 atgaagaaaa tcatttgtct ggtcattaca ctactaatga cactccccgc ttacgcgaag
    12121 ttaactgccc atgaagaagc ccgcatcaac gccatgctgg agggattagc acagaaaaag
    12181 gatttgatat ttgtgcgcaa cggtgatgaa catacctgcg atgaagcggt ttctcatctg
    12241 cgtctgaagc tgggcaatac ccgaaaccgc attgacactg ccgagcagtt tattgataag
    12301 gttgcttcgt cgtcatcgat taccgggaag ccgtatattg tgaagatgcc cggtaaaagc
    12361 gatgagaacg cacaaccttt tttacatgcg ctaattgcgc agacggataa aacggtgcct
    12421 gcgcaataat cttaaatccc cgccccctga ctaaaatgct cttccccaaa caccccggtg
    12481 gaaaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc agggttagct
    12541 tttgccaccc gcagtgctcc ggcaaccgcg ccgccggagc tgacgccgca gaatattcct
    12601 tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat atccagcacc
    12661 tcatccacca gagaagcgtt gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg
    12721 ccgggaatgc tgctgccctc ttccggttgc aggccgacaa tggtcaccgg tttggattgt
    12781 tcgcgcataa agcgtgacac gccggtgata gtgccggttg ttcccatgct ggagacaaaa
    12841 tgggtaatgc gcccgccagt ttgctgccag atttccggcc cggtagtggt gtaatgggcg
    12901 taagggttat cgggattatt gaactgatcg agcagctttc cttcaccacg attcgccatc
    12961 tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag aatcagttcc
    13021 gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg catcagcaat
    13081 ttcatgcgat agcctttaag cgcggcaatc attgccagcg caatgccagt gttaccacta
    13141 gtggcttcga ttaacacatc acccggttta atttccccgc gcttttccgc ctcgacgatc
    13201 atcgaaagcg ccgcacgatc ttttacagaa cctgccgggt tattgccttc cagttttaac
    13261 cacacttcac tgccgttatt cggccccatt cgctgcaact tcaccagagg cgtattgcct
    13321 attgtttgtt ctaatgtact cacgatctct atccatacgt ggtgtcgcct gatgcgacgc
    13381 ttacgcgtct tatcaggtct acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg
    13441 gaagcgcctc cgggcgttta acattcaatc aacctatcag gcgctttgtg cgagagcaag
    13501 ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat gttgcagacc
    13561 aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg
    13621 ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt tcgggctggc
    13681 ttccagtacc tgtaccggca gcggcgaatc gaggctggta cggcggctga tatccacttc
    13741 ccaagggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc ccagcggcca
    13801 gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc
    13861 gcccataaat tcgagcacaa aacgggtcgc cggttcgcgc catacctgat ccggcgcgtc
    13921 agcctgttca atattgccct ggctcatcac aactacacga tcagctactt cggtcgcttc
    13981 ttcctgatcg tgggtcacaa aaacgctggt gaattttagt tcttcatgga gttgacgcag
    14041 ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccaaacggtt catcaagcag
    14101 cagaatttgc ggttccacag ccagcgcacg cgccagcgcc acacgctgtt tctggccgcc
    14161 ggaaagctgc gccggataac gatccgccag atgagcaagc tggaccattt ccagcaattt
    14221 tgtcactttc gctttgatcg ccgcggcatt cgggcgctcg cgacgcggca gcaccgtcag
    14281 gccaaaagcg atattgtcga acaccgtcat atggcggaac agcgcgtaat gctggaacac
    14341 gaaaccgact ttacgatcac gtgcgtgcag gcggctcacg tcggtgccgt ggaagcgaat
    14401 atgcccgctg gtttgatgct ccagcccagc gataatgcgc agcagcgtgg ttttcccgga
    14461 accggacggc cccagcaacg cgaccatctg acctgaagga atatccagtg agatatcgtt
    14521 cagcacctgg gtgcgaccaa acgacttctt aatattggca atctcaatgc tcatgatgtt
    14581 cctcctgctg tgcgcgtttt tcctgattct ccaggcgcca ctgcaacata ctttttaaaa
    14641 acagggtgat aatcgccatc agcgttaaca gcgccgcagc ggtaaaggag ccgacggtgt
    14701 tgtagtcctg ctccagcaat tcaatctgta acggcagcga cagggtttcg ccgcgaatcg
    14761 agccggaaac caccgacacc gcgccaaact cgccaatcgc gcgggcgttg gtcagcacca
    14821 cgccgtaaag cagcgcccag cgaatgttcg gcaatgtgac gcggcgaaac atctgccaac
    14881 cagacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt aacatcaccg
    14941 gcaccagctc gcgcaccaca aacggacacg tcacaaagat ggtgaccagc accattcccg
    15001 gccaggagaa cataatttgc agattatgtt catccagcca gccgcccagc gggccgttag
    15061 agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacggca aacggaatgt
    15121 ccagtagcgt cagcagtaac tggcgtccag ggaagttaaa gcgcgtcacc agccaggcca
    15181 gcagaatgcc gaacaccagg ttcaccggta cggcaatcag cgcaatcatc accgtcagcc
    15241 aaatcgcgtg caacatatcc ggatcggcca gattctgtaa aaccggcatc agccctttgc
    15301 tgaacgcctg tacgaagata taaatcatcg gcaccagcag gatgaatgcc gataccagca
    15361 tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca taacgcttca
    15421 attgtgtcac ttccgccatt aatgacctac cacacgccga ccaaagcgac tttgcagagt
    15481 gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaggcaa tcgcgctcgc
    15541 tgccgggtaa tcaaactcct gcaagcgcac aaaaatcatc agcgacgtca cttccgtctt
    15601 ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa
    15661 cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa
    15721 actctgccag cgcgttgcac caagcgtttc cgccgcttct tcatattccg ggcctaactc
    15781 ttccagcacc ggctgcacgg tacgcaccac aaacggaatg ctggtaaagg ccatcgccac
    15841 cgcaatccca agccaggtgt aggtgacttt gatatcaaac ttcgccagcc attcaccgta
    15901 aaaaccgttc acggaaaaga gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc
    15961 aaagggtaaa tccatcagcg catcaagcag cgtgcggcca gggaagcgat aacgagttaa
    16021 gatccacgcc atcagcaggc cgaacacgcc gttaaaaatc gacgccacaa acgccgacag
    16081 cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacttccc agtactgcgc
    16141 ccagctcatc tcagaaagct gcatcaccag cgcggaaagc ggcagcagca atatcaggca
    16201 cacaaacagc agactggtgc cgaggcttaa ggtaaagccc ggcaggacgc gtcttgatga
    16261 aactgcaaac attaattacg ccccgccgct aacagcttgt ctaactcgcc gccgctggtg
    16321 aagtgggttt tcatcacttc cggccaggag ccaaatttgt cttccacgcg gaacagctcg
    16381 gtctgcggga atttgttttt cagtttgtcc atgacttccg ggttatttac gcggtagtaa
    16441 tagtcggtga tgatggtctg cgcctgcggg ctgtagagcc agttcaggta ggcttttgct
    16501 gctttttccg taccgttggc ctgcacgttt ttatcgaccc acgccaccgg gaattccgcc
    16561 agaatgttgg ttttcggaat caccacttca aagccctgcg cttcatactg tttgcggatg
    16621 ttgttcactt ccgactcgaa gctgatcagc acatcgccca ggccgcgctc ggcaaaagtg
    16681 gtggtcgcgc cacgaccgcc agtatcgaac acttcaacgt ttttcaggaa ctgggtcata
    16741 aactgttcgg ttttggcttt gtcgccaccg tcagctttat ccgctgcgcc ccatgccgcc
    16801 agataggtat aacgcgcgtt acccgacgtt ttcgggttcg gaaaaatcag cttcacgtcg
    16861 gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg caccaggaag
    16921 cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca gtccgccggg
    16981 ataagcttgc ctttatcgtg cagaatttgt acgtcggtca cctggttata agtgacaacg
    17041 tcggctttta agccctgcaa aattgccagc gcctgttttg atgacccggc atgagattgt
    17101 tttatcgtca gtctgtcgcc gccgttatct ttggcccatt gctgttcaaa cggcggggtc
    17161 agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc cgttgcctgt
    17221 acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt cagtaagtta
    17281 acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat atttataacg
    17341 gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagttg aaaagggaat
    17401 aagacgaaat cctccccaca aatgcaggga ggaaacagag gtgaatcaga taccgacgct
    17461 gaccgtctcc ggcagtgtac tgccgccatc aatcacattc tgtgtaccgg ttaaatagct
    17521 ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat cggcgaggcg
    17581 acgcagcggg attgctttcg ccatttcagt cagcaccgat tctggatctt ccgggttcga
    17641 ctggcgggca atgctttccg ccattggtgt gcgaacgtat ccagggcaga tggcgttaac
    17701 gcgaatacca gactgcgcat actccaccgc cagcgatttt gtcaggccaa caatcgccgc
    17761 tttcgttaag gcgtacgccg tttcacccgg atcgaccacc atatcaccag tgactgaaga
    17821 catcatcaca atgcgaccat ctttgcgggc aatcatctcc ggcagcaccg ccttcgtgac
    17881 gttccatacg cctttaatat tgatgtcaat atggaaatcg cgatcgtcat cgctcatatc
    17941 gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa tgcgcccttc
    18001 tttttccttc gcgcgtttga tagctgcggc taccgacgcc gggtcacgca catcggcgac
    18061 aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gccagctttt cgatctcagg
    18121 ggagatatcc agcaagatta ggtttgcgcc atgacgtgca aaagttctgg caattccttc
    18181 gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt tacccatttt
    18241 aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat acaggataaa
    18301 caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag tcgctgcttt
    18361 atgcatcatt tcaggcgttg tgttagctca ctgctgcgct gaatcatttt caatgactcc
    18421 acatcattga gttgcacaag accaacaaac agtaaatcgg taacagaatt ttgcgccgtg
    18481 cgggtagaca tcgacgagct acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa
    18541 tgcgccagcc gccgcagcgg agagtcagcc agagaagtaa tggcaatcac cgttgccccc
    18601 tgctttcgtg ccgcttctgc gcacaagaca atctcttttt tactgccgct gtaagagata
    18661 gcgatttgta catcgccttt tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg
    18721 gcttcgcagg ccacgcgata gccaattttc atcagtttga acgacagatc gcgccccacc
    18781 agcgccgatc cgcccaggcc cgttatctgg acaaacggtg ctttactgat gacatcaatg
    18841 attttttgca gccgcgcgta gtcgaacaac gcgcaggttt gctccagcgc cagctctttt
    18901 tcgcgattca gtttgcgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc
    18961 aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc tattaacgcc
    19021 atacgcaatt cagtaaatcc ttgtgcgccg agcttctggg caaacttcac aatactcgac
    19081 tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag ctcactcacg
    19141 ttggcccgca gaaagtcagc aattttttgc tcgttctcgg taaattcact ttccgcattg
    19201 cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa aattccgctt
    19261 aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc tttaagtctg
    19321 tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat agtaaggtca
    19381 ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcctc ggctgaaatt
    19441 gaccgcgtat caacgttgga aatgtgccgg attatcaacg atgaagataa aaccgtaccg
    19501 cttgccgttg agcgcgtact gccggatatc gccgcggcga tcgatgttat ccacgcccag
    19561 gtcagcggcg gcggtcgtct gatttatctc ggtgcgggaa catccggtcg tctggggatt
    19621 ctggatgcca gcgaatgtcc gcccacctac ggcgtgaaac cgggtttggt ggttggtttg
    19681 attgctggag gcgaatatgc cattcagcac gcggtggaag gcgcggaaga tagccgggaa
    19741 ggcggcatta acgatctgaa aaatattaat ttaacggcac aggatgtggt ggtcggtatt
    19801 gccgccagcg gtcgtacgcc gtatgtgatt gccggactgg aatatgcacg ccagctcggc
    19861 tgccgtacag tgggaatttc ctgtaatccg gggagcgccg tttccaccac cgctgagttt
    19921 gctattacgc cggtggttgg ggccgaagtg gtcaccggtt cttcgcggat gaaagcaggc
    19981 acggcgcaga aactggtgct caatatgctt tccaccgggc tgatgattaa atccggcaaa
    20041 gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag
    20101 gtcaatatcg ttaaaaacgc caccggatgt agcgcagagc aagcggaagc ggcgttaatt
    20161 gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct cgatgccgct
    20221 gaagctaaaa aacgcctgaa tcaacacggc ggatttattc gcaaggctct ggaaaaggaa
    20281 taactcatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc
    20341 ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat
    20401 gacagttcac tggttgatcc cgatatcaaa acgctggaag gcgtgaaggg cgtcattttg
    20461 accagcgacc aggtgcaggt ggtttttggg cctggtaaag cgcatcgtgc cgccaaagcg
    20521 atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc cgctcagaac
    20581 aaacgtcagt taaaagccaa acaaacctcc ggtgtgcagc aatttctcgc caaattcgcc
    20641 accatcttca cgccgctgat ccccggtttt attgccgccg gtctgctgct ggggatagcg
    20701 acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgacgcg
    20761 ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcctggtgat tctggtgggc
    20821 tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc
    20881 tttttgctcg gttataaccc aactgccacc acgggttact acgccggttt tcacgatttc
    20941 tttggtctgc ccatcgatcc acgcggcaat attatcggcg tgttgattgc cgcatgggcc
    21001 tgcgctcgca ttgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgttg
    21061 acctcgctaa tcactctgct gatcaccgct acgctggcat acctgatcat tatgccgctg
    21121 ggtggctggt tattcgaagg catgtcgtgg ctgtttatgc acctgaacag taatccgctc
    21181 ggttgtgcgg ttttagccgg actgttcctg atcgccgtgg tgtttggcgt acatcagggc
    21241 tttattcctg tttacctcgc gttaatggac agtcagggat tcaacagctt atttcccatc
    21301 ctttcaatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa
    21361 ccgcacagtg cattacgcag tcaggtacgc ggggcgatta ttcccggtct gctgggcgtt
    21421 ggtgaaccgt tgatttacgg cgtcaccctg ccccgcatga aaccgtttat taccgcctgt
    21481 ttaggcggcg cagcgggcgg tttgtttatc ggcctgatag cctggtgggg tctcccgatg
    21541 ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc tgccgctgat gacttccgca
    21601 caaggcatcc ttccggcaat ggcggtttat gctggcggga ttctggtggc atgggtttgc
    21661 gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg atattatgaa
    21721 acggacaatg ctctatcttt ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata
    21781 ccctgttctg acagaaagtt cgcccgagaa agcagggttc aacgtcgaac ggcttaacca
    21841 gatggatcgc tggattagcc agcaagtgga cgccggttat cccggcgtaa acctgctgat
    21901 tataaaagat aatcagattg tgtatcgcaa agcctggggg atggcgaaaa agtacgatgg
    21961 tagcgtgtta atggcgcagc cggtaaaagc caccaccgac acgctgtatg atctggcctc
    22021 aaacaccaag atgtacgcca ctaacttcgc cctgcaaaag ctaatgagcg aaggcaagct
    22081 gcatcctgac gatcggattg cgaaatatat tccgggattt gccgatagtc caaatgacac
    22141 catcaaaggc aaaaacacgc tgcggatttc tgacctgctg catcacagcg gcggtttccc
    22201 ggcggaccca caatatccga ataaagcggt cgcgggcgcg ttatattccc aggataaagg
    22261 ccagacgctg gagatgatca agcgcacgcc gctagagtat cagcccggca gcaagcatat
    22321 ttacagtgat gtcgattata tgctgcttgg atttatcgtc gagtcggtca ccggtcagcc
    22381 gcttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacac atacggtatt
    22441 taacccgctg ctgaaaggct ttaaaccgca gcaaattgcc gccacggaat tgaacggcaa
    22501 tacccgcgat ggagtgatcc acttcccgaa tatccgcacc tccactctct ggggtcaggt
    22561 gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcacgcag gtttgttttc
    22621 caataccggc gatattgcgg tgttaatgca aacgatgctg aacggcgggg gctatggcga
    22681 tgtgcagctg ttcagtgcgg aaacggtgaa gatgttcact accagctcta aggaagacgc
    22741 cacttttggc ctcggctggc gcgtgaatgg taatgccacc atgacgccga cgtttggcac
    22801 gctggcaagc ccgcaaacct acggtcacac tggctggacc ggaacggtga ccgttatcga
    22861 tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc cggttgccga
    22921 tccgcaaaag aatcccaata tgttcgaaag cggtcagctg ccgattgcaa cgtatggttg
    22981 ggtagttgat caggtgtatg cggcgttaaa gcaaaagtaa taaaaaggcc agtcagtaat
    23041 gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca
    23101 gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac
    23161 gcttaccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg
    23221 cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct
    23281 ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg
    23341 tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca
    23401 tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca
    23461 caaacacata gctgccgccg gcatccacgc cgtctttgat aaccgccact tcgcgacgcg
    23521 tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca
    23581 cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg
    23641 ccgcctgggc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat
    23701 cgaactgggt ggtcggtgca aggcctttac cgtagcccgg aaaatctttc agctcttctg
    23761 ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa
    23821 gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg
    23881 ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc
    23941 aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataataatg
    24001 ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt tttttcgcgg cggcggtttg
    24061 ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt tcaaggtatc
    24121 gtccgaaggc attaaccctt caggaccact ccattccccg ctgaactggt agctaatatg
    24181 ttgactgcct tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca
    24241 attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc
    24301 agcaggaata tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat
    24361 caccatcgcc acgttgtcgc ccttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc
    24421 ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcggcaa tggcttgttc
    24481 ttgcagtggt gtggacgccg ttaattcacc aacgccctgc tcgctcactt tggtggacga
    24541 cccaaaccag ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc
    24601 ggtcagcatc aatggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt
    24661 ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attcttttac
    24721 ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc
    24781 cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg
    24841 ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagta cacgccaggt
    24901 gcgaggaaac gtagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaatgccagg
    24961 aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc
    25021 ctgctcacgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag
    25081 cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg
    25141 gaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt
    25201 catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc acattgatct
    25261 gaattttcgg gcagccacga gcaatcagct ttttctccag ccgattaagc aacgcattgg
    25321 caatcccacg cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc
    25381 cgtcataacc gcccatcacc gttccgacca cttcaccgtt tacctcagcg accagaaaca
    25441 aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca
    25501 gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc
    25561 gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg
    25621 tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca
    25681 actattgaaa tgaaaagtaa aacaattctc aacagcaaac ggtcgtaacg gattacgcga
    25741 tacgatataa catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc
    25801 actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg ccctgacgtt
    25861 gtcaggaatg tcgcaagcca tcgccaaaga cgaaccttta aaaaccagca acggacacag
    25921 caagccgaaa gctaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg
    25981 cgggattgat accggagcga tcgggcgcaa cggttcgaaa gaaaaacatg tggtgctggc
    26041 gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg
    26101 ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc
    26161 agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc
    26221 ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg
    26281 cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca
    26341 gcaagtgctg tttgatctgg tgcaaaccga tactattaaa aacagcctga cgctcggctc
    26401 gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc
    26461 cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat
    26521 caccaacccg gaagaagagc gactgttagg ctcggcggcg tttcgtcaga aaatcgccac
    26581 agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc
    26641 gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct
    26701 tcaggatacg atttgtcagc agttgaccgc cgtcgatggc acagaatttg tcgaagatag
    26761 ctggcagcgc gaaggtggcg gcggcgggcg tagtcgggtg ttgcgtaatg gtggtgtttt
    26821 cgaacaggca ggcgtcaact tttctcacgt ccacggcgag gcgatgcctg cttccgccac
    26881 cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt
    26941 gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcgctttt ttattgccga
    27001 aaaaccgggt gcagagcccg tctggtggtt tggcggcggc ttcgacttaa cccctttcta
    27061 tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gcctgccatt
    27121 tggagaagac gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca
    27181 tcgcaacgaa cagcgcggta ttggcgggct gttctttgat gatctgaaca cgccagattt
    27241 cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc
    27301 aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta
    27361 ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcactc tgtttggcct
    27421 gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga
    27481 gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt
    27541 cagggattgg gtgtaactcc ctcaccccat tcccgcatcc gctgatgcaa cgtcaacgac
    27601 ggcttctcgg caaacagctg ctggtaatcc gtggcaaatt gccccagatg ccagaatccc
    27661 cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc acgggcttat cagttcgcgg
    27721 cgtacggcgt tcaggcgaat gcgtttcagc cacgcgttcg ggccaatgcc taaaatagcg
    27781 tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt gattacacaa gtccagcacc
    27841 gtcaccggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag taatctacgg
    27901 taactctgat gactgatgct ttccgccgtc accattggtt gcgcttcttc cagcatcgcc
    27961 cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg atgaagattt
    28021 tccgggttct cgcaaaacgt cgccagcgcc tgttggacaa agccccacag cgcggcttta
    28081 tgctgctctt tcacttccag cgccgactgg ctacgcaaca tatgtaatac ccgatccgga
    28141 ttatgcaaaa agttagcctg acgggtgatg acatcttcag aaagcactac gcccaggatc
    28201 gtgtaatcgt ccggcgtact taattcaaac tcggttccac cagggcgcgt cgctatttct
    28261 gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc cggaatgcca
    28321 aaccagaacg agttcggcca gaccagacac gactgacgca gcgccagacc ggtgtattca
    28381 cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa cttgcccgga
    28441 tgcagctgat cgtaaatctg ctgccaggcg gtaatcgtta aagcatgttc atagacatcc
    28501 gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt caggttttcg
    28561 ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt catgatgtta
    28621 atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt gagacgataa
    28681 cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc atttctggct
    28741 ttttccagcg gccagccaaa gtgggcagcc acttctcctg ccgtcattcc ctgacgtact
    28801 gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg cgcgtcgggt
    28861 tcctttacgg gttgcttcgg ctggcggtta aagccagtaa ccagccactg ggtgtcatcg
    28921 tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc ggcagcacaa
    28981 cctgcgtcca gcgcagcacg acacgccgcc agatcgcctt ccaccaccag cgttaagcga
    29041 ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag catggcatcc
    29101 gctgcatcta tcgcagcgac catgccgtcc acttccagca atcccagtgc attgatcatc
    29161 ggcaacctcc attacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta
    29221 aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag
    29281 ttagtttcag acggtggcgg gacataggtc accagctgta cgtcggcaga tttcaacgct
    29341 gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca
    29401 agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac atgtgccagg
    29461 aatgccgtat cttgcgcatc attagcccac tggaaagcag cgccattttc aatatgcgca
    29521 accatcgcat ccagacctgc acgcacttcc gccgggtttg gcccaccaag cataatcagc
    29581 acttcaccgg cagtcggtga cgggccgtgt gcagcgcccg catacagcga gcggccatac
    29641 accacttcca ccatcgcctg cttggtcgct tcatcagcgg caatgtaggt gacgtcatcg
    29701 gagtcggcag aaatcagacc gaggctacga atatgcggcg gcaattttag ttcacgcgca
    29761 aaatcggcgt taacggaggc aatcacccgc atagcggtga cagacggtcg aatcaaatct
    29821 aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc
    29881 atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt gccgccctgg
    29941 tgaatgttag agatacaggt gcgatcggcc tcaacggtgg tcgccatacg cggcgagtaa
    30001 acggcgtagc aggagaggct ttctgactga cccaaccccg gacgttcgcc caccagcagg
    30061 atcactactt tcgcgccgag gatctcaccg atctgatctt caatcttcac gcgaccataa
    30121 cgaacaaaga atggcgtgcc gactttcagc ccggcctgtt tcaggcccgc catcagcggc
    30181 ggcaggatct cttcgtagtt cacagtgatg gcatcagttg acaggccatc agaaataacg
    30241 acctgtacgt ccggattagc aacacactgt gctttcagcg cttcaacagc ttctgcacac
    30301 aggcggcggc ccatatccgg gcgagtcagg tagagatttt tgtcgctaat ttcggagcgt
    30361 acttccagca agccctgcgc tttcacccac tcttccggca cttctttcag tacggtatct
    30421 ttcgaacgag agtgatcggc caggaaacgc agcagcgcca gcgtacgcgg acgcggaccg
    30481 gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa cacatccgca
    30541 cgatgtgggt tttcaacacc aatccacgct tttgcttccg cagaacctaa atccagcgcg
    30601 cagctttccg aggtcaccgg tgccgcacag gtggtagtgg cgcactttgc ttctgacggc
    30661 gcgggggccg tttgtcccat tgacgccatc acgctgcgta caatttcttc aatctgtttt
    30721 tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg cccgcccgtt
    30781 tggtcaagcg accgtttgcc ataatgccca tgctttccag ccagcgttca aactccggtg
    30841 acgggcgcag gttgagtaac tgacgcacag tggcagtgtc gtggaaggcg gtggtctggt
    30901 agttgagcat gatgtcatcg cccaacggca tccccatgat gtagttgcag cctgcggtgg
    30961 cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg ttggtgtaac
    31021 agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct tctaagcccg
    31081 cacggataat ctggcggtcg ttgtagagat actccggccc aataaaaccg accacggtgt
    31141 tgacgataaa cggatcgtaa tgacgtgcca gcccatagtt acgtgcttcc atcgtcacct
    31201 ggtcagcgcc gaagttagcg ccagcggata gcgcagagcc ttgtccggtt tcgaagtaga
    31261 ggcagttttc cccggcgata cggttgaact ctgcgcccac tgcgcgcgct tcgtcgagca
    31321 tcgccagttc cacgccaaac tctttcagcc ctttttcgct gccacagata ctctggaaaa
    31381 tcagtccgcc aggcgcgccg cgacgaatcg cttcgatctg ggtggtgacg tgcgccagta
    31441 cgcagccctg agttgggatg ttgaatttgt cgatcacgcc ataaatggta tccagcacgc
    31501 ggcttaagtt ttccacgtcg tcagttaccg ggttgacgcc gatcaccgca tcgcccaccc
    31561 cgaaggaaag cccttcgtag atttgcgcgg cgatactttg cacgtcgtca cgagtgtcat
    31621 ttggctgtaa acgggcgctg aaggtgcccg gaataccgat ggtggtattg gcctttttga
    31681 ttaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc ttcgctaccg
    31741 ccgcgaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc acgctggttt
    31801 catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc tggttgtagg
    31861 ccgtttcgtt aacatcgtcc tgaatcagcc gcgtcacgca gtcatcttca taggcaatca
    31921 ccggattatt gcggatgtcc gctacggtca tttccgacaa cacctgcttt gccgccacgc
    31981 gctcctgtga acttgccgct gcaacgcctg ccagcacatc ccccgaacgc agttcgttgg
    32041 ctttagccag cacctctttt acatccttaa actgatatac attgccgaac aatgtggtct
    32101 ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg tcaccggcac
    32161 aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac
    32221 ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca tgcccagagc
    32281 tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa aacgcgcgac
    32341 gaaatcgacc aacgcgttga tgaccgtcag taccgcggcg taacgcacag gcagcgaggc
    32401 gggaagcgcc agcacgtacg cgtcagtttt gggatcaaga tccagttgaa tcagcgcctg
    32461 ttgccaggca ctcaccagat ccgtttcatc aatcgggatc gccaccggca aattgcgcag
    32521 cggcagttgt acgccctcaa gccagattgt gctgccagag agcgaaaggg tatgtgcccc
    32581 cgcgccaatc accgtggcac gcacggtttg cgccggaaac tgcacgttca tctcacgcag
    32641 gcgcggatgg tcatgcagcg ccgttgccag cagcgggcca atatcggcaa aacagaacgg
    32701 gtcggcgggc tggtggcgat aacattcgcc cacgccgcca gaaagcgtaa tgatttcggg
    32761 cgtaacacct gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg gcgagagcgt
    32821 tccgtcaatc acttcgacaa tcagttctgc catccgccgg gtaacctgca ccagctgcgc
    32881 gccggtcagc gaacggacgt cagtgcctgc accgaagcac tcatccacaa tcatctgccc
    32941 cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac
    33001 gttgaggcag gcggtgccgc tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc
    33061 accgccgatg tcgatattca gtacccgaca cagccgttgt tcagaaaggg tttgtgcccc
    33121 ggctccgtga ccggcgatca cggattcgag gtgcggcccg gcgctggcaa cgacaaaatc
    33181 gcccagcgac tgagagagcg ccatcaccgc cgggcgagca ttgcgggttt tcgcgctttc
    33241 accggtgatg atgatggcac cagaatcaac gctttccggc tcaatacccg cagcctgata
    33301 ttgctcgagt attaaggttt tcagttccgc ttcttttaaa ccgccctgtt tatcgacagg
    33361 ggtaaagaac accggacttt gccagctaat ttcgcgttta atgaattcgt agcgcggcac
    33421 ctgcgacacc gccgcacggt taaccagctc caggtgggag aaaatcacct gggtggtggt
    33481 ggtgccgata tcgataccga cgctcaatag ctggcgagtg ttcacgattg cgcctccgct
    33541 tcggttttag ccgtggtcgc gtcttctttt ggcaccagca tcatcgccac gccaatcgcc
    33601 gttacgccgc cgatcaattt gccgacaatc atcgggaaga tcatggcgtt catgttggca
    33661 gcggcgaagc ctaagtggtc gcccagggcg aaagccgcgg aaacggcgaa ggcgcagttg
    33721 atcactttgc cgcgggtatc catctgcttc atcatgccga acatcggaat gttgttggca
    33781 agcgttgcca ccatgccggc tgccgcgatg ttgttcatat tcagtacctt accgacgctc
    33841 atcagcggtt tttcaaacca gcgggtcagc agcagcacca tcggatacgc ccctaacaga
    33901 acgcaggaga tagaaccgat aacttcaatg gcgcgcatca cctcaccggg tttatcgcca
    33961 ggggccataa agataggatc cagaccgggg atcagttccc agccaagcag gaatttcact
    34021 accgcagcgg caagaccgag ggtgatcaat gcaacgagga atttggcgaa gatctggaag
    34081 ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagaat cgcaacaatg
    34141 atcaccggga tcatgttcat caggatcagg gcgaaagtga attccaccgg ctggccgttg
    34201 atctgaacac cggagtacat agctaccaga ccaccagcga tacaaccaat cggaatggtc
    34261 acaatgcccg ccagcacgcc gagcgccaga taacgacggt cagaaggttc gataatgccg
    34321 agcgccaccg gaatggaaaa cacaatcgtt ggccccatca tcgacccgag aattaaccca
    34381 gagtatagcc acgctgctac gtcgccgccc gccagctctt tggcgaggaa gaagccgccc
    34441 atatcgcacg ccagcagcgt tccggcgaac attgacgggt ttgcgccgag catttcgtaa
    34501 accgggataa ttaccggccc gagtacgtga gccagtaccg gtgccagcgc ggtcataccg
    34561 accatcgcca gacccagagc gcccattgcc ataaagcctt cttcgaactg accgcctgat
    34621 ccttcgatac ttttaccgaa cttaccgagg aaacgagcag aaccgccgaa ctgcgacagg
    34681 atcctgtcta cggcagctat cagcataaag aacatcatga tgtacatgat gatttcgtta
    34741 attcccatcg cctttactcc ctgttagttg ttatttatta gcggatgcgg cgtgaacgct
    34801 ttatccgctc tacatgtgca atcccgtagg ccggataaga cgcgacatgc gtcgcatccg
    34861 gcatttattg cgccgctgcg tacaggccga caatctgttc cagactggcg gtacgcgggt
    34921 tgctgcgcag acaaatatct tcctgcgcgg cctgcgccca tgcaccgtaa tgcgcagatg
    34981 tcgcgccaac atcgcccagt cgtttaccaa tcccaacttc cgcaatcagc tcacttaccg
    35041 cgttaatagc gtcacgatcg tcggattttt tagttcgcag tgcccgacca atctgactaa
    35101 agcgttcacg acaaaccatc cggttaaact ccatcaccgt tggcagcaac atagcgttcg
    35161 cgagaccgtg tggaatatgc agcgccgcgc ccggctgatg cgccatcgcg tggcacaacc
    35221 caagacccgc actggaaaac gccattcccg ccatacatga agccagcaac atgctctcgc
    35281 gcgcggcaag gtcgtgaccg tagcccaccg ctttcggcag cgatttgcca atcatcgcaa
    35341 tggcaccaat cgccagactg tcggtaaacg gtgtagcgtt cagggcgctg tatgcttcaa
    35401 tggcatgggt taacgcatca atgccggtca tcgccgtgac atgcgacggc acaccttcgg
    35461 tcaatgcggc gtcgaggatc gccacatccg gcatcagcga ggcatgggct aacacctgct
    35521 tgcgcccgct caccgcgtcg ataatcaccg ttacattggt ggtttcagag ccggttccgg
    35581 cggtagttgg aatggcaatc agcggcaagc gcggttgcag aacgctggtt tctgacatct
    35641 ctgccagcgt gctatccggg ttcgtcacca gcaacgccac ggctttcgcc gcatccagca
    35701 ccgagccgcc gccaaatgcg atcaccccgt cacaacctga ctcacgcaac tgcgccacgg
    35761 ctgcacacac gtcggtaatg cacggttcgc ccaccggaca tggccagagc gtcatggcga
    35821 tacctttaac ggccaggcta cgcgtcagcc cggcggtcat ccctgcctga tgcaaaaagc
    35881 tgtctgccat cacgaacaga tgtttcagcc cacgcgtttg cgcttgctgt ccgcaactgc
    35941 tcaccgagcc cggaccgcaa agcgtcaccg gtggaacgct aaatgttttt acccgttgca
    36001 gattcagggt atcgaacgcc tgaaagagcg cggtctgcaa ttcattttgc atatagcccc
    36061 tccgctttct ctcgcccact actggcgatc gccagcgggg tcataaacaa actgtgctgc
    36121 ggtaaatgca cctgtaacgc cgggaattgt ttgcgaaaca gttccgccac gcccggttgc
    36181 atacaagagc cgcccgccag ccataaatca gtaatgccct gcccttcaat atgccgggcg
    36241 acaatgtccg ccattttttc gtacaccggt ttcaccgcag gccaaatctc ttcaccgtga
    36301 ccgcgcttgt actgctccgc ctcttccagc gaaatacggc gatttccggc gagagtcaga
    36361 gagatgtgat gtccgccagt cgcttcatcc gccgagtacg tcaccttgcc ctttttcacg
    36421 atggcaatgc cggtagtgcc gccgccgata tccactacac cggcgttgtc cagttgcagc
    36481 agatccgcaa ctgcggttgg ctcatccagc acatggctca cttccagtcc ggcagattcc
    36541 agcacgttga tggaaatacg cgggtccgta cctggaggaa atgaagtcgc cgcatggcta
    36601 aaacggcgac cgaattgctg ctcaagagtg tcgagatggc gacgaacaat ggtgacagcg
    36661 ccgaagaaat cccagacgat gccgtcgcgg accacatcgg cccagtcgag gcataccgcc
    36721 actggctgac cgtcgcggtc gacaaccatc gacaccacat cgcaggtgcc cagatccacc
    36781 cccagccaca gcggagattc cgtcgcggcg ggcgtctggt tacaccgcgt tgccgccgtt
    36841 tgcagacgtg gggtgagcca ttgttcgtcg tgcgccatct gttactcctt aaacaatgcg
    36901 aaacgcatcg actaatacac agcgacgcag acggacaaac gtacgcgcgc tggttacccc
    36961 ttcaccggtt ggcgtggtga tggtcatggt ggtccagcct tccccgccca gccccagccc
    37021 ggcaatgcac ggtccgttct taacgaaaat gctggtatca atagcattcg ccatctggtt
    37081 catgttttcg atgttgcgcg agtgcattgc cgccgtgtgg tggcaaccgc cctccagttt
    37141 caccgccagc gcaatggcat ccgccacgtt ggcgacgcgc acgacgggca gcaccggcat
    37201 catcagttcg gtcacggcaa atggatgttc tgcagtggtt tccacaaaca gcaaacgcgt
    37261 ttcttgcggc acgttcaggc cgattgccgc cgcgattttg cctgcgtcgc gaccaaccca
    37321 gtcacggctg acggtgcctt ttccgcgctc gtcgatattt ttcagcaaca ccggttgcag
    37381 ctgctgcgcc tgttctgcgg tcagtttcac cgcgtgctgg ccttccatca gacgcatcag
    37441 ttcatcggct acgctatcaa caacaatcag taccttttcg tcggcacaaa tgatgttgtt
    37501 atcgaaagac gcgcctttga cgatggactg agcagcacgg gcgaggtcag cggtttcatc
    37561 caccactacc ggcgggttgc cagcgcctgc ggcaatcaga cgtttattgg tgtgtttacg
    37621 cgccgcttct actaccgctt cgccgccggt caccaccagc aggccgatac ccggaaactt
    37681 gaacaagcgt tgcgcggttt cgatatccgg atttgccaca gtaaccagta agttttccgg
    37741 cccacctgcg gcaacaatcg cctggttgag cagcgtaatc gcccgctggg agactttttt
    37801 cgccgccgga tgcggggcaa aaatgacgct gttgcccgcg gcaatcaggc tgatggcgtt
    37861 attaattacg gttgccgccg ggttagtgga cggcgtcacc gaagccacca cgccccaggg
    37921 cgcgttttca attaaagtca ggccgttgtc gcccgtcagc acttgcggag agagacactc
    37981 aacgcctggc gtgccgcgtg cctgagcgac gtttttggca aatttatctt caacgcgacc
    38041 catgccggtt tcactgacgg caagttccgc taaatctctg gcgtgttttt cgcctgcttc
    38101 acgaatggca gcaatggcta actggcgcat tgccacgctc tttaaccctt gctgggcaat
    38161 tttggctgcc gcaacagcgt catccaggga cgcgaaaacg cccatctcat gaacggcggc
    38221 gggcggcgtg tcactgcttt gcattttcag cagtaccgct ttcaccacct gttcaatatc
    38281 ctgttgattc atgatgttct gccttatttg tggaaaatta cctgaccgcc agacaccacc
    38341 tcatcgacga tgccaatcac gcacagatcg accggtgacg tttcgctttt atgcgcctgg
    38401 cgggcggaac tgccactcac cagcaacacc cactccccgg ttcccgcgcc aatattgtcg
    38461 atggcgacgg cacattgccc gtcaggatta ccttgtggat caatcatttc caccatcagc
    38521 aatttgtcat gcgccagtcc gtgatggcgt acggtacaaa caatttgtcc agtgacgact
    38581 gccagtttca tacccgcctc cgtggcgtat ttcaggtaaa agctccccct accctccgcg
    38641 gaaggtaaaa tgaaaaatga gagagcgtga cgcccgaatc gacgtcacac agggtgatta
    38701 caggttgctg ctatcgcctt tcaggccgat cgggaagact tcttccaggt cgccgtgcgg
    38761 acgcgggata acgtgtacag ataccagctc gccgatacgc tgtgccgctg cggcaccggc
    38821 atcggtcgcc gctttacagg ctgcaacatc accacgaacc atggcagtac acaggccgcc
    38881 gccaatctgc ttaacaccaa ccagcttgac gcgcgcagct ttcaccatcg cgtcagaagc
    38941 ctcaatcagt gcaaccaggc cccgggtttc gatcattcct aatgcttcca ttgtgctttc
    39001 ctctttatca gggtccagaa cgggaccgtt cattcaacca gtgtttgtaa actgctttcg
    39061 cggttcactt ctgtctgacg cggcacagct gccaccagcg ccagctcgat aatttcctgc
    39121 acgctacaac cacgagagag atcgtgcatc ggcgcggcaa gtccttgtat caatggcccg
    39181 acggcacgat atccgccaag tcgctgtgcg attttgtaac caatatttcc ggcttccagc
    39241 gacggaaaaa ccatcacatt ggccttgccc tgtagcgggc tggcaggcgc tttttgcgcc
    39301 gccacttccg gcacgaaggc ggcgtcaaac tgcaactcgc catcgacaac cagctttggt
    39361 gcgcgctcac ggacgatttc tgtcgcctgc tggacgttgg caacgcaggg gtgacgggcg
    39421 ctaccgttac tggaaaacga cagcatcgcc acgcgcggct cttctccggt gatggcgcgc
    39481 caggtttcgg cgctggcaag cgcgatatcc gccagctgcg ccgccgtcgg ctgtggtacc
    39541 acgctgcaat cggcaaagcc caacgcgggg ccgctgtact gtggcagcat caggaaaatg
    39601 gatgagagcg ttttacagcc aggctgcaag ccaataatgc gtaatccggc acgcagcaca
    39661 ttcgccgtgg aagagagatt gcccgcgata cagacatccg ctttaccggc gctgaccatt
    39721 gcggcggcga acatcagcgg atcggtaagt ttttccagcg catccggcgg cgttttttcg
    39781 cccgcgcggg ccagccagcg atgagcaaat tcttcccgca ttgcgaggtt gccatgcgga
    39841 tcaatcacct gtagcccgtc catcgctacg ccatgactga gcgcaaactg acgaagttca
    39901 aacggattgg cgaccagaat gggcgttgcc agaccttgtt gatgtaaata ttgcgcagct
    39961 ttcagcacac gttgatctaa cgcatccgga aaaaccactc tggcgggcgc tcgcaacgcc
    40021 agttcacgac aacgttcaat aatcattgcg tttcccccag tcgttgttgg atctgcttca
    40081 ccgttagcgg ctgcttcgtt acgctcagga gcatcatcac gtagaccgtg ctcgaaaggc
    40141 ggttcagcgc ctgcaagatg tccgggcgta atacttcaaa gctgcgggtg ataaacacct
    40201 gcgccgctac ggtttcggtt tcccgcactt tggtgcgtag cagattgagc aacgcagcat
    40261 cgcgcccgtg gctggcttcc ggcaccagat gatcgtggtc gagatagcgc agcggctgat
    40321 gagaaagccg atgcagatct tcgtcagaaa gcccgacaat cgcttgatcg cccagcggtt
    40381 cccccagcgc atcggcacgc ataatgttgc caagacgcga acggatatcc gcaagccacg
    40441 gctgccacgg ttccgccagt tcaatttgca gccacacagc cagcgcaatg gtgctgtcga
    40501 gaaccgcgcg aaaacccaga cgcggatcgc ttttggcaac cattttttcc gccgacagat
    40561 gagtcagcgt atccggcttt tttgccaccg gctggtggca cagttcgcag cacgcctgcg
    40621 gatgttcatc gctgctggtc agcccatgaa cgggctgcgg ctgctgctgt tcatcgtcaa
    40681 caaacaggcg gccctgctcg tcaataaact tgatgcgcag atggcggctt tccagcaact
    40741 cccgggcaga gggcgtcagg cgactgtccg cagggagatg gatctctgcg ccttcgctga
    40801 gcgtatggtt cgctcttagc catgcttcgg tgatgaaatc tttcataggg attgccagtt
    40861 agccggccag gcgacataca ggaatttcac gctggatgtc gtaccaaatt cgatgctgga
    40921 gcctttcggg ataaacatta cgtcgcctgc tttggcgatc atggtttcgc cttcgtggcg
    40981 aacatgcagc tccccttcca gcaccatgtc gatttcgtcg tagttcaggg tccacgggaa
    41041 gaatgcgttc tcccactgca taaaaccggc ggccatgctg ctgccatcgt ctccggtcac
    41101 aagatcagtc aagcccacgc aatgcggctc tgcgccgtcg aaacgaccaa acttgacgct
    41161 gctaccatca atcactttga tgccgccttt gccggtcacc gatttgaagc tcggctgcat
    41221 tgcaccctgc tccagcgact gtttttcctt catcactttt tccatcaact gcgcgaccag
    41281 gctttcggta aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctgact
    41341 ttcggtttta tcggctggca cgctggcggg aacggacgcc gttaccgggg acgattcatc
    41401 acattcggtg atggtaaaac ccagtagatc cgccacttca cgggcttccg gggtaataat
    41461 gctggcgcgc agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc
    41521 attcgctgtg atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat
    41581 ccaccagttg ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga
    41641 agcccgtctc aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt
    41701 tactgatgac ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc
    41761 gactttccgg gtcattcgcg ccgtggacat agatcagcat gtccacatcc tgtagcgtgg
    41821 taattaaggc gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc
    41881 ccttatcatt aaattccacg gcctgtgttt ttctggcgag ggtataattc ccctgcaacg
    41941 cattaaataa cgttgttttc cctgccccga cggtacccac aaacgcaatt cgtttcatga
    42001 atgcctcgta ttaacttttg gtcatttcac aaagcgtata atttaataag agacctaaac
    42061 cactgacggt ttgtgataac gcttcttcta ctgcacccac cgagccataa atcaccagcg
    42121 cgccgctaaa acggtcgaga aaaccgatat gcacatcggc agctttcagc gctaaatcac
    42181 cggcgatcat cgccgtttcg ccaggagtta gcgtcataat gccaattgcg cccgcatccg
    42241 gaacgccgat tttcttcgcc agttcctcgc cgggatgcgc gatgagatgc gccagtgtga
    42301 cttgtttacc gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt
    42361 gaaaaacttt cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc
    42421 gcgtttaaaa ggtgaggtag atcactaaga atggagagat attcgctgtg gtgcataaac
    42481 tttcatatgc aacgcatgaa tataaataaa ataatatcga gatattatat agactgcttt
    42541 tttttattat tcgcaaactc agtaataagc tttactaata tattgcagcc aaaccgctta
    42601 ccctgaatat tcagggtaag cgtgagagtt aaaaaagaat tacagcggtt gggtttgcgc
    42661 ttcaaccacg gccagcgcca ccatgttgac gatacgacgt accgatgcga tcggcgttaa
    42721 cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct
    42781 ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgtttggca ttaccagaat
    42841 attggcggaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag
    42901 cgctgcatcg ccgtgcattt caccatcaat catcagttct ggcgcacgtt ccctgaccag
    42961 ttccagcacc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg
    43021 cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg ccgccatcaa
    43081 ggtgatctcc gccagctctt ctgcatccgg ttcatcatta acatatgtat cggcaataaa
    43141 ggtgttacca ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa cgccatcgcg
    43201 ataaccaaag acatttttca ccacgctaaa atgttcatga taatcgccca ccgtaccgca
    43261 gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg tcgggttact
    43321 gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga tctggaagta
    43381 ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgatct caaaatcaac
    43441 gcccgatttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc
    43501 gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtggcat gcagaacgcg
    43561 cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat
    43621 cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc
    43681 gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgctttcg cgaccgcagg
    43741 agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt ccggaccaaa
    43801 gctcagatcc tgatcgccat acgctgaagc caccacttcg ctctgttccg catgggccag
    43861 ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac
    43921 gtccagcgcg ccacggaaga tgaacggaaa gcaaaggacg ttgttgacct ggtttggata
    43981 gtcagaacga ccggtgcaga tgatggcatc cggacgcact tctttcgcca gcggcggcag
    44041 aatttccggt tccgggttcg ccagcgccag gatcattggc gctcgagcca ttttcttcac
    44101 catttcctgg gtcagcactt tcgggccgga acagccgagg aaaatatccg cgccttcaat
    44161 cacatcatcg agggtacgtt tgccgtcatc cactaccgca tatgcggctt tggtttccgc
    44221 catgtttggc tcacggccct gatagataac gccttttgaa tcgcaaacca cgatgttatg
    44281 tttttgcaga cccagcgcta ccagcaggtt catacaggcg attgctgcag cacccgcgcc
    44341 ggaaaccacc atacgcacgt cagagatgtt tttctccacc acgcgcaagc cgttgaggat
    44401 ggcggcagtg ctgataattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat
    44461 ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt
    44521 gatgccgccg aaggtcggtt ggagcgcagc gacgacttca ataaatttgt ccgggtcgag
    44581 ttcatcaact tcaatgtcaa atacatcaat tccggcgaat ttcttaaaca gaacgccctt
    44641 gccttccatc accggtttgc ccgccagcgc gccaatgttg cctaacccca gcaccgccgt
    44701 accgttagag atcaccgcca ccaggttacc gcgcgcggta tatttgtagg cttttaacgg
    44761 gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc
    44821 gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc
    44881 atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa
    44941 cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc acattattac
    45001 cttgccggag tctatagact ttgagcaagt ccaaactctc accattaata taatgttttg
    45061 gtaataatcc tataacactg atgttacctg cttaatccag caataccatg cctgtctgct
    45121 atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa
    45181 cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc
    45241 actgtcgtgg cagacagcgg cgatattgag tccattcgcc attatcatcc ccaggatgcc
    45301 accaccaatc cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata
    45361 gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca
    45421 gcgtgtgaca aactggcggt caatttcggt gctgaaatcc tcaaaatcgt acccggtcgc
    45481 gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg
    45541 cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tctgatcaag
    45601 ctggcttcga cctgggaagg aattcgcgcg gcagaagagc tggaaaaaga aggtattaac
    45661 tgcaacctga cgctgctgtt ttcttttgca caggcacggg cctgtgcgga agcaggcgtt
    45721 tttctgattt cgccgtttgt cgggcgtatt tatgactggt atcaggcacg caagccgatg
    45781 gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat ctacgactac
    45841 tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa
    45901 atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct gaaggagctg
    45961 caggaaaaag tttcgccagt ggtacgtaaa ttaatcccac cttctcagac gttcccacgc
    46021 ccagctccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc gatggcggta
    46081 gaaaaactgt ctgaaggcat tcgtctgttc gccgttgatc aacgcaaact ggaagatctt
    46141 cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa
    46201 tgcgattcgc gcactcagta tggatgcggt acaaaaagcc aattctggtc atcccggcgc
    46261 gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta aacataaccc
    46321 taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc acgcgtcgat
    46381 gctgctctac agtttgctgc atctgaccgg ttacgacctg ccgctggaag agctgaagaa
    46441 ctttcgtcaa ctgcattcga aaacccctgg ccacccggaa atcggctata cgcccggagt
    46501 tgaaaccacc accggtcctc ttggacaagg tttagcgaac gccgtcgggc tggcgatagc
    46561 ggaacgtacg ctggctgcac agtttaacca gccagaccat gagatcgtcg atcacttcac
    46621 ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag tctgttcgct
    46681 ggcgggcacg ctgggactgg gcaagctgat tggtttttac gatcacaacg gtatttcgat
    46741 tgatggagaa accgaaggct ggtttaccga cgatacggca aaacgttttg aagcctatca
    46801 ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg aagcgatcct
    46861 tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgtcgtacgg tgattggctt
    46921 tggatcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccgctgg gggaagaaga
    46981 agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga
    47041 tatttatcac gcctgggatg cccgcgaaaa aggcgaaaaa gcgcagcaga gctggaatga
    47101 gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagttta tccgtcggat
    47161 gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca atgagttaca
    47221 ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg
    47281 gccgatacta ccggagctgc tcggcggttc ggcggatctg gctcccagca acctgaccat
    47341 ctggaaaggt tctgtttcgt tgaaggaaga tccggcgggc aactacattc actacggggt
    47401 gcgtgaattc ggcatgaccg ctatcgccaa cggcattgcg caccacggcg gctttgtgcc
    47461 gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc
    47521 actgatgaaa gcacggcaga ttatggttta tacccacgac tcaattggcc tgggcgaaga
    47581 tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag
    47641 cacctggcga ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg cggttgagcg
    47701 ccacaacgga ccgacggcac tgattctctc caggcagaat ctggcccagg tggaacgtac
    47761 gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa
    47821 gccagatatt attttgattg ccaccggttc agagatggaa atcaccctgc aagcggcgga
    47881 gaaattagcg ggagaaggtc gcaatgttcg cgtagtttcc ctgccctcga ccgatatttt
    47941 cgacgcccag gatgaggaat atagggagtc ggtgttgcct tctaacgttg cggctcgcgt
    48001 ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga aaggggcaat
    48061 tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc cgttctttgg
    48121 ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg
    48181 atggtgatcg ccggatgctg attgccggat gcgacgctga cgcgtcttat ccggcctaca
    48241 gcgtcttatc cggtctacat gtccccgcta ttttgtttaa cgggtgatcc acaacgtggg
    48301 ccaggcatct ggcccatgcc agttatcgca ggtcggttct gcggcgtaac gcaccaggcg
    48361 aaaacgctga ccgtcaaagc gccagcgcgc ctgaatgcca caatcgctta atccgcgccc
    48421 tttcgctaag gtcaccagct cacgcgattt ctcatcaaat gttgcgttca tcagttccag
    48481 ttcattcgta tcctgaccgt tgttgaacgg caaacgcaac cgaaccgggc gcgaggccag
    48541 cggcttttta cgcgacacaa tccatgccaa atcaatggtg ttataggccc ctgcctcaca
    48601 gctaatcatc atcagcgctt tatcatcagt cagcgcagtg acattcacct cacgacgcaa
    48661 tggatcaagc gagcagcgca gaccattcat ccgccagttt ccataatcca gcaaatcgtt
    48721 gcgttcttcg agtgagagtg gcgtcggcgt tgggttaacc accacgacct ctttcagcgc
    48781 aggcgcgggc ggtacgctga gcggcggttc gtctcctttc tttatccagg cggtttcact
    48841 gccaacgcgc ttttgctgag catcaataaa caacaacgcc gcttttaagc cgctcagaga
    48901 aatggtctga ttgccgtcgc gtaaggtgat cgccttccct tcctgaatca tctgcaaaaa
    48961 ctcggtgatg gttgccgtat catcggttac taataaccat ggtgaaatcc gccacttgtc
    49021 accacttaac gccaacggct caccatctaa cagcagccgt ggcgctatct ccccttcaga
    49081 cgcatccggc gatttcaacc cgccgcgctc aatacgtaaa acggcatcgg tatgcgcccc
    49141 ggcgctgcgg ctcagggtca tcaccagtcc attatgatcg cccgtattgc gcgccacgca
    49201 gaagttttgg ttattacagg tgacctgcca gtcggaaaac gcccgttgcg ttggtgccgc
    49261 ccacaccaaa gacgtcggca acaaagcgaa aaaaaagaga aggaaaatgc gatagcgcat
    49321 ggacggtacg accccagaag ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt
    49381 agcgcggctc aatcggattt gtcggattga gccaacaata aacaacgaaa tattattgtt
    49441 atttttcagt ccattaaatg tgacatttgc aaatagttaa gcaataacac cgtcttaccg
    49501 tcacgtatct cgccggtttt gatcatctcc agcgcctggc tgaacggcag ctcgagcact
    49561 tcaatatctt catcttcgac accgccaccg gcgttagcgc gctgattgtc actgtattcg
    49621 gcgataaaaa agtggattag ctcagtcaca ccgccaggcg acatatacag ttcaaataat
    49681 ttgcgcactt cgcccacttc atagcccgtc tcttcaatcg cttctttacg aatgcacact
    49741 tccggttcgt cgttatccag cagcccggcg caggtttcaa tcagctgccc gctttcattg
    49801 ccattaaccc aggtagcgac acggaactga cgaatcagaa ccacggtctt tttcttcgcg
    49861 ttgtacagga ggatcgtcgc accattaccg cgatcgtaaa cttcacgttt atggcggata
    49921 acttcgccat ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa atagttatcg
    49981 gagagaattt tgtctttaat gagggtgatt tgttgcgtca taccgactcc acagcgcgaa
    50041 atgaacaatt atattacgct gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt
    50101 gactgatttt acccccagcc agtcaataat cccctgtgcc gcatgacgtc cttctgccat
    50161 tgcggtaacc accagatccg caccacgcac ggcgtcgcca ccggcgaaga ttttcggatt
    50221 ggtggtctgg taacggtact ggctttccac atccgcgatg atgcggcccc atttgtctac
    50281 cgttacaccg tgcgactcca gccacggcat cccgtgcgga ttgaagccaa acgccataat
    50341 caccgcgtcg gctggcatga caaattcact gccttccacc ggcactggac gccgacgccc
    50401 ctgggcatcc ggctcgccaa gacgcgtgcg caggaaacga atcccgcaga cgtgaccttg
    50461 ttcattcagc tcaagcgcca ccggctggac gttaaattcg aagttggccc cctcttcgcg
    50521 ggcgttcttc acttctttct tcgagcctgg catgttagct tcatcacgac gataagcgca
    50581 ggtgacgtta ctcgcgccgt ggcgcagtgc ggtacgcaca cagtccatcg cggtgtcgcc
    50641 gccgcccagt accacgacgt taagtccggc ggtattgata aacggctctt ccggtagctc
    50701 ttcgagcccc atcacctgtt tagtgttggc aatgaggaac ggcagcgcgt cataaacgcc
    50761 cggcgcatct tcattgggta aacccgcttt catggaacgg taagtgccta cgccaacgaa
    50821 gaccgcgtcg tattgttcca aaagcgaatc caaagagaca tctttaccca cttcacaatt
    50881 gagttcgaag tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca gcagggattt
    50941 atccagtttg aaagatggaa tgccgaaagt gagcaagcca ccgatttctg gatggcgatc
    51001 gtacaccgtc acccccacgc cattgcgggt cagaacatcc gcacaggcca gccctgccgg
    51061 acctgcaccg ataatcgcca cccgcttgtc cactttggtg acatggctta agtcaggacg
    51121 ccaacctttc gccaacgcct gatctgaaat gtagcgttca atgttgccga tagttaccgc
    51181 accgtgctca tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg gacaaacgcg
    51241 tccggtaatt tccggcaggg tgttggtctg gtgagaaagc tcgacggcgg cgtcgatgtt
    51301 tccggctttc accagttcaa tccactgtgg aatatggtta tgcagcgggc aggtccattc
    51361 acaaacgctg tgctcgccgc acttaaggca gcgcgaggct tcccgttgtg cctggtcggc
    51421 gcgaaatggc agataaattt catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc
    51481 cggctcgcca cgcgcgggcg ttgcctgcat ttgttcgact ttactcatta ccggcatttc
    51541 ttgtgcagcg gtactggcat gccacggttg atgctcctga cgtgcggtgc gcaagcggcg
    51601 ggatttcgcc atgccggaga gtgcggcatc agtgaccagt tgcagcgcgt ctgccgggca
    51661 attctcgaca caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt
    51721 ggcttttact ttgcccaccg cgacgggtgt caggacgatt tgcatcgtac caaacggaca
    51781 ggccaccacg caggatttac agccaataca cttttgctga ttgacctgaa tgctgtcatc
    51841 aacgtggctg attgcgccat tagggcagct acgggcgcag ggcgcatctt cacaatggtg
    51901 acaggtcact gcactacgtt gctgttgatg tttgataacc gtaattcggg gatgaaaatg
    51961 gtgttggctc aggacatgtt gctcatcatt gtgagccatg acacaggcga tttcacaagc
    52021 atgacaaccc agacattgct gactgttggc cataataaaa cgattcataa cgaccttctt
    52081 ttttggttgt aaaaacctta ttctttatat gagt
//
LOCUS       DAZUGK010000009        49621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZUGK010000009 DAZUGK010000000
VERSION     DAZUGK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.009"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            113..1234
                     /locus_tag="ACHY5W_000452"
     CDS             113..1234
                     /locus_tag="ACHY5W_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS4445416.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSDNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLLAIFPNPQQQLL
                     PGMFVGARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1234..4341
                     /locus_tag="ACHY5W_000453"
     CDS             1234..4341
                     /locus_tag="ACHY5W_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFS4445417.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPGLAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIILT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            4345..5718
                     /locus_tag="ACHY5W_000454"
     CDS             4345..5718
                     /locus_tag="ACHY5W_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFS4445418.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            5727..6890
                     /locus_tag="ACHY5W_000455"
                     /pseudo
     CDS             5727..6890
                     /locus_tag="ACHY5W_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(6941..7747)
                     /gene="sapF"
                     /locus_tag="ACHY5W_000456"
     CDS             complement(6941..7747)
                     /gene="sapF"
                     /locus_tag="ACHY5W_000456"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFS4445419.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DLVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(7749..8741)
                     /gene="sapD"
                     /locus_tag="ACHY5W_000457"
     CDS             complement(7749..8741)
                     /gene="sapD"
                     /locus_tag="ACHY5W_000457"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFS4445420.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(8741..9631)
                     /gene="sapC"
                     /locus_tag="ACHY5W_000458"
     CDS             complement(8741..9631)
                     /gene="sapC"
                     /locus_tag="ACHY5W_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFS4445421.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(9618..10583)
                     /gene="sapB"
                     /locus_tag="ACHY5W_000459"
     CDS             complement(9618..10583)
                     /gene="sapB"
                     /locus_tag="ACHY5W_000459"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFS4445422.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(10580..12223)
                     /gene="sapA"
                     /locus_tag="ACHY5W_000460"
     CDS             complement(10580..12223)
                     /gene="sapA"
                     /locus_tag="ACHY5W_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFS4445423.1"
                     /translation="MRQVLSSLLVIAGLVNGQAIAAPEPTPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(12534..12779)
                     /gene="ymjA"
                     /locus_tag="ACHY5W_000461"
     CDS             complement(12534..12779)
                     /gene="ymjA"
                     /locus_tag="ACHY5W_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFS4445424.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHDMAAEAGINSARIDEIAEFLNQWGNE"
     gene            complement(12912..14297)
                     /gene="puuP"
                     /locus_tag="ACHY5W_000462"
     CDS             complement(12912..14297)
                     /gene="puuP"
                     /locus_tag="ACHY5W_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFS4445425.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLVRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(14287..14451)
                     /locus_tag="ACHY5W_000463"
     CDS             complement(14287..14451)
                     /locus_tag="ACHY5W_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445426.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(14600..16018)
                     /gene="puuA"
                     /locus_tag="ACHY5W_000464"
     CDS             complement(14600..16018)
                     /gene="puuA"
                     /locus_tag="ACHY5W_000464"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFS4445427.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRCLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            16230..16994
                     /gene="puuD"
                     /locus_tag="ACHY5W_000465"
     CDS             16230..16994
                     /gene="puuD"
                     /locus_tag="ACHY5W_000465"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFS4445428.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            17021..17578
                     /gene="puuR"
                     /locus_tag="ACHY5W_000466"
     CDS             17021..17578
                     /gene="puuR"
                     /locus_tag="ACHY5W_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFS4445429.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            17728..19215
                     /gene="puuC"
                     /locus_tag="ACHY5W_000467"
     CDS             17728..19215
                     /gene="puuC"
                     /locus_tag="ACHY5W_000467"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFS4445430.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARSVFERGDWSQAAPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCSDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            19217..20497
                     /gene="puuB"
                     /locus_tag="ACHY5W_000468"
     CDS             19217..20497
                     /gene="puuB"
                     /locus_tag="ACHY5W_000468"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFS4445431.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDIARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            20535..21800
                     /gene="puuE"
                     /locus_tag="ACHY5W_000469"
     CDS             20535..21800
                     /gene="puuE"
                     /locus_tag="ACHY5W_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFS4445432.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRLGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(21920..22897)
                     /gene="pspF"
                     /locus_tag="ACHY5W_000470"
     CDS             complement(21920..22897)
                     /gene="pspF"
                     /locus_tag="ACHY5W_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFS4445433.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGCQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            23064..23732
                     /gene="pspA"
                     /locus_tag="ACHY5W_000471"
     CDS             23064..23732
                     /gene="pspA"
                     /locus_tag="ACHY5W_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFS4445434.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            23786..24010
                     /gene="pspB"
                     /locus_tag="ACHY5W_000472"
     CDS             23786..24010
                     /gene="pspB"
                     /locus_tag="ACHY5W_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFS4445435.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            24010..24369
                     /gene="pspC"
                     /locus_tag="ACHY5W_000473"
     CDS             24010..24369
                     /gene="pspC"
                     /locus_tag="ACHY5W_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFS4445436.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            24378..24599
                     /gene="pspD"
                     /locus_tag="ACHY5W_000474"
     CDS             24378..24599
                     /gene="pspD"
                     /locus_tag="ACHY5W_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFS4445437.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            24674..24988
                     /gene="pspE"
                     /locus_tag="ACHY5W_000475"
     CDS             24674..24988
                     /gene="pspE"
                     /locus_tag="ACHY5W_000475"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFS4445438.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            25199..26878
                     /gene="ycjM"
                     /locus_tag="ACHY5W_000476"
     CDS             25199..26878
                     /gene="ycjM"
                     /locus_tag="ACHY5W_000476"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFS4445439.1"
                     /translation="MKQKITDYLGEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFKDFFIAVDP
                     KTDLRAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDIAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITDLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            26892..28184
                     /gene="ycjN"
                     /locus_tag="ACHY5W_000477"
     CDS             26892..28184
                     /gene="ycjN"
                     /locus_tag="ACHY5W_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4445440.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGRAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            28205..29086
                     /gene="ycjO"
                     /locus_tag="ACHY5W_000478"
     CDS             28205..29086
                     /gene="ycjO"
                     /locus_tag="ACHY5W_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4445441.1"
                     /translation="MNRLFSGRSDMLFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIEPTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            29073..29915
                     /gene="ycjP"
                     /locus_tag="ACHY5W_000479"
     CDS             29073..29915
                     /gene="ycjP"
                     /locus_tag="ACHY5W_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4445442.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLSQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            29946..30998
                     /gene="ycjQ"
                     /locus_tag="ACHY5W_000480"
     CDS             29946..30998
                     /gene="ycjQ"
                     /locus_tag="ACHY5W_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4445443.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDHAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            31016..31804
                     /gene="ycjR"
                     /locus_tag="ACHY5W_000481"
     CDS             31016..31804
                     /gene="ycjR"
                     /locus_tag="ACHY5W_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4445444.1"
                     /translation="MKIGTQNQAFFPENIREKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEVLAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            31814..32869
                     /gene="ycjS"
                     /locus_tag="ACHY5W_000482"
     CDS             31814..32869
                     /gene="ycjS"
                     /locus_tag="ACHY5W_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4445445.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNVLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDIARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            32866..35133
                     /gene="ycjT"
                     /locus_tag="ACHY5W_000483"
     CDS             32866..35133
                     /gene="ycjT"
                     /locus_tag="ACHY5W_000483"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFS4445446.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHFDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLESAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDVA
                     FIHRAEMFLKELWLPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLVDF
                     ILPFNGTATTYQEDE"
     gene            35130..35789
                     /gene="pgmB"
                     /locus_tag="ACHY5W_000484"
     CDS             35130..35789
                     /gene="pgmB"
                     /locus_tag="ACHY5W_000484"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4445447.1"
                     /translation="MKLQGVVFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQYGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRPLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLRSTDSLTWSRLSAFWQN
                     V"
     gene            35803..36885
                     /locus_tag="ACHY5W_000485"
     CDS             35803..36885
                     /locus_tag="ACHY5W_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4445448.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGVLNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            36930..37835
                     /gene="ompG"
                     /locus_tag="ACHY5W_000486"
     CDS             36930..37835
                     /gene="ompG"
                     /locus_tag="ACHY5W_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFS4445449.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDESGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(37946..38944)
                     /gene="ycjW"
                     /locus_tag="ACHY5W_000487"
     CDS             complement(37946..38944)
                     /gene="ycjW"
                     /locus_tag="ACHY5W_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4445450.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            39100..40497
                     /gene="ycjX"
                     /locus_tag="ACHY5W_000488"
     CDS             39100..40497
                     /gene="ycjX"
                     /locus_tag="ACHY5W_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFS4445451.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            40494..41555
                     /gene="ycjF"
                     /locus_tag="ACHY5W_000489"
     CDS             40494..41555
                     /gene="ycjF"
                     /locus_tag="ACHY5W_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFS4445452.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAKNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            41703..43244
                     /gene="tyrR"
                     /locus_tag="ACHY5W_000490"
     CDS             41703..43244
                     /gene="tyrR"
                     /locus_tag="ACHY5W_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFS4445453.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(43288..43794)
                     /gene="tpx"
                     /locus_tag="ACHY5W_000491"
     CDS             complement(43288..43794)
                     /gene="tpx"
                     /locus_tag="ACHY5W_000491"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFS4445454.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            43913..44878
                     /gene="ycjG"
                     /locus_tag="ACHY5W_000492"
     CDS             43913..44878
                     /gene="ycjG"
                     /locus_tag="ACHY5W_000492"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFS4445455.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(44853..45581)
                     /gene="mpaA"
                     /locus_tag="ACHY5W_000493"
     CDS             complement(44853..45581)
                     /gene="mpaA"
                     /locus_tag="ACHY5W_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFS4445456.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(45890..46528)
                     /locus_tag="ACHY5W_000494"
     CDS             complement(45890..46528)
                     /locus_tag="ACHY5W_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4445457.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRKEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(46549..47478)
                     /locus_tag="ACHY5W_000495"
                     /pseudo
     CDS             complement(46549..47478)
                     /locus_tag="ACHY5W_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(47478..48398)
                     /locus_tag="ACHY5W_000496"
     CDS             complement(47478..48398)
                     /locus_tag="ACHY5W_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4445458.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQHPAIVVSHPGG
                     GVKEQTAGAYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            48536..49435
                     /gene="pgrR"
                     /locus_tag="ACHY5W_000497"
     CDS             48536..49435
                     /gene="pgrR"
                     /locus_tag="ACHY5W_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFS4445459.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLRSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        12075 a        12340 c        13284 g        11922 t
ORIGIN      
        1 ccttatttca ttgcgaaatt catttcgcga tgcgggttcg ttataactga atatccctga
       61 tatatcctca ttgattttaa tgaggagggt atttctcacc ctggagaaaa taatgaagta
      121 tatagcaaca tctgtagtgg caatgctgct cttatctggt tgtgataata cgcaaagtga
      181 caattcatcc ccgtcagaaa cagaagtcgg cgttgttaca gtcaaatctc aaccggtctc
      241 ggtagtcagt gaattaaccg gacgcaccag tgctgcgctc agtgccgaag tacgtccgca
      301 ggttggggga attatccaga aacgcttatt taaggaaggt gatctggtca aggctggaca
      361 gccgctctac cagattgatg cggccagtta tcaggctgca tggaatgaag ctcgggctgc
      421 attacaacaa gcacaggcac tggtaaaagc cgattgccag aaagcgcagc gttatgcccg
      481 actggtgaaa gagaacggtg tttcacaaca ggatgctgat gatgctcagt ctacctgtgc
      541 acaagataaa gccagtgtag aggcgaaaaa agccgcactg gaaactgcgc gcattaatct
      601 tgactggacc acggtaaccg caccgatttc agggcgcatt ggcatttcgt cggtaacccc
      661 tggcgcactg gtgaccgcgt cgcaagatac agcgttaacg actattcgtg gtctggatac
      721 aatgtatgtc gacctcactc gctccagtgt cgatttatta cgtctgcgta aacagtcact
      781 ggcgaccaac agtgacacca tgagcgtctc acttattctg gaagatggca caacctacag
      841 cgaaaaaggg cgtctggaac tcaccgaagt cgctgtagat gagtctaccg gttcggtgac
      901 attactggcg attttcccca atccacaaca gcagttatta ccgggaatgt ttgttggcgc
      961 tcgtgtcgat gaaggcgtga tggaagacgc tattctcgcg ccgcaacagg gcgtcacgcg
     1021 cgatgctaaa ggcaatgcaa ctgcgctggt ggtgaataaa gacaataaag tagagcagcg
     1081 aacgctcgaa acgggagaaa catatggtga taaatggctg gtgctaaacg gcctgcacaa
     1141 cggcgaccga ctgattgttg aaggttctgc caaagtcact tcagggcaga cggtcaaggc
     1201 tgttgaagtt caggctaatg gaggcaacgc ctgatgtttt cgcgcttttt tgttcgccgc
     1261 ccggtctttg cctgggttat cgccattttg attatgttgg cggggattct ggctattcgc
     1321 acattgcccg tcgcgcaata tcctgacgtt gcgccaccga ccattaaaat ttcagccact
     1381 tatactggtg cttctgccga aacgctggag aacagcgtga ctcaggttat cgagcaacaa
     1441 ctcactgggc ttgataattt actctatttc agctcaacca gcagctctga tggttcggtc
     1501 agtattaatg tgacctttga acaaggtacc gatccagata ctgcacaggt tcaggtgcag
     1561 aataaaattc agcaggcgga gtcgcgccta cctagcgaag tgcagcaaac gggtgttacg
     1621 gtggagaaat cacaaagcaa ctttttgctg attgctgccg tgtatgacac cactgacaaa
     1681 gcctctagtt cggatatcgc cgactggttg gttagtaacg ttcaggaccc gctggcgcgt
     1741 gttgaaggtg tggggagtct gcaagtcttt ggcgcggaat acgctatgcg catctggctt
     1801 gacccggcca aactggcgtc ttactcgcta atgccttcag acgtgcaaag tgctattgaa
     1861 gcgcaaaacg tgcaggttac tgccgggaaa ataggggcat tgccttcacc gaatactcag
     1921 caactgaccg caacggtacg tgcgcagtct cgcttgcaga cggtggatca gttcaaaaat
     1981 attatcgtga aaagccagtc agacggcgca gttgttcgta taaaagatgt ggctcgcgtt
     2041 gagatgggca gtgaagatta taccgctatc ggcaaactta acggtcaccc gtctgccggg
     2101 gttgctgtaa tgctttcgcc cggtgcgaat gcgctgaata cggcgacgct ggtcaaggat
     2161 aagattgccg aattccagcg gaacatgccg cagggatacg acatcgctta cccgaaagac
     2221 agcactgaat ttattaaaat ctctgtagag gacgtaattc agacgctgtt tgaagccatc
     2281 gtattggtgg tttgcgtgat gtatttattc ctgcaaaacc tgcgtgccac actgattccg
     2341 gggttagctg tccccgtcgt tttgctgggc acattcggcg ttcttgcgtt gtttggctat
     2401 tcgattaata ccctgacgtt gtttgcaatg gtgctggcga tcggcttgct ggtggacgat
     2461 gccattgtgg tagtggagaa cgtcgaacgc attatgcgtg acgaagggtt gcccgcgcgt
     2521 gaagccacgg aaaaatcaat gggcgagatt tctggcgcac tggttgccat tgcgctggtg
     2581 ttgtcagccg tattcctgcc tatggccttc tttgggggat ctacgggggt aatttatcgt
     2641 cagttttcga taaccattat ctccgcaatg ctgctttccg tggtggtggc attgaccttg
     2701 actcccgccc tgtgcggttc cgtcctccag catgttccgc cacataaaaa aggctttttc
     2761 ggcgcattta accgctttta ccgccgtact gaagataaat atcagcgagg cgtaatttat
     2821 gtcctgcgcc gtgctgctcg aacgatgggg ctttatctcg tactgggtgg tggaatggcc
     2881 ctgatgatgt ggaaactgcc gggcagtttc ttacccactg aagaccaggg cgaaatcatg
     2941 gtgcagtaca cgctgccggc cggggcgacc gctgcccgta cagcagaagt gaatcgccag
     3001 attgttgact ggttcctgat taacgagaaa gcaaataccg atgtcatttt taccgttgat
     3061 ggtttcagtt ttagcggcag cggacagaac accgggatgg cgtttgtttc gttgaaaaac
     3121 tggtctcaac gtaaaggggc agaaaacacc gcccaggcta tcgccctacg ggcaaccaaa
     3181 gagctgggca caattcgtga tgccacggta tttgcgatga cgccgccagc cgttgatggg
     3241 ctggggcaaa gcaatggttt tacgtttgaa ttgttagcta acggtggaac cgatcgcgaa
     3301 acactactgc aaatgcgtaa tcagttgata gaaaaagcga atcaaagtcc ggagttacat
     3361 tctgttcgtg ccaatgattt gccacaaatg ccgcaattgc aggtagatat tgatagtaat
     3421 aaagcggtgt cattagggtt gagtttgaat gatgtcaccg acaccctgtc cagtgcgtgg
     3481 ggcggtactt atgtgaatga ctttattgac cgtggtcgtg tgaaaaaagt ctacattcag
     3541 ggcgacagtg aatttcgttc cgcgccgtca gacttaggta aatggtttgt gcgcggtagc
     3601 gataacgcca tgacaccatt ctctgctttt gcgaccaccc gctggctgta tggaccggaa
     3661 agactggtgc gctacaacgg ttctgcagcc tatgaaattc agggggaaaa tgcgactggc
     3721 tttagttccg gcgatgcaat gacaaaaatg gaggaactgg caaacagtct tcctgcggga
     3781 acaacctggg cctggagtgg tttgtcattg caggagaaac tggccagcgg tcaggcattg
     3841 agtctgtatg cagtttctat tctggtggtt ttcctctgcc ttgcagcact gtacgaaagc
     3901 tggtcagtcc cgttctcggt cattctggtg atccctctgg ggcttcttgg cgcggcgctg
     3961 gcggcctgga tgcgtgattt aaacaacgat gtttacttcc aggtggcgct attaaccact
     4021 atcggcttgt cgtcgaaaaa cgccattctg attgtggaat ttgctgaagc ggcggttgca
     4081 gagggttatt ccttgagtcg tgcggcatta cgcgcggcgc agactcgttt acgcccaatc
     4141 atcttgacct cgctggcgtt tattgcgggg gtaatgccgc tggcgatagc aaccggcgca
     4201 ggggcgaaca gccgcatcgc cattggtacg ggcattattg gcggtacgct gaccgctacg
     4261 ttgctggcta ttttctttgt tcctctgttt tttgtactgg tgaaacgttt gtttgccggt
     4321 aaaccgcgcc gtcaggagta agttatgttg cgtcgttctc ttatttttct ggtgctgttg
     4381 agtgcgggat gtgtctcgct cgatccccac tacagcacgc ctgaatcccc aatcccggcg
     4441 accttgccgg gagcacaggg ccagggaaag gcaatcagcc atgactggca gcaggtgatc
     4501 cacgatcccc gattgcagca ggtggtgact attgcactga acagtaaccg cgatgtgcaa
     4561 aaagcgattg ctgatattga ctcagcacgg gcgctctatg ggcaaactaa tgcgtcgtta
     4621 ttcccgacgg tgaatgcggc cttaagttca acccgcagtc gttcgctggc gaatggtacg
     4681 gaaaccactg ccgaggctga cggcacggtg tccagtttta cgttagatct gtttggtcgt
     4741 aatcagagtt tatctcgggc ggcgcgagaa acctggcttg ccagtgaatt tacagcccag
     4801 aacacccgtt taacgctgat tgctgaaatc agcaccgcct ggctgacact ggcggccgat
     4861 aacagcaacc tggccctggc aaaagaaacg atgaccagtg cagaaaactc attgaaaatt
     4921 atccagcgcc agcaacaggt tggcacggcg gcagcgacag atgtcagtga ggcgatgagt
     4981 gtttatcagc aagcgcgcgc cagcgttgcc agctatcaga cacaagtgat gcaggataaa
     5041 aatgcgctga acttactggc aggtacaacg cttgaggaaa atttgctacc gggaacactg
     5101 gaaagcctgc cggaacagat gataagtctg gttcctgcgg gagtgtcatc tgacgttctg
     5161 ctgcgtcgcc cggatatcca ggaagcggaa cataatttga agagcgccaa tgccgatatt
     5221 ggcgcggcgc gggctaactt tttcccgacg atttcgctga ctgccagcgc tggtgttggc
     5281 agtgacgcat tgtcgtcgtt gttcagccac ggaatgcaga tctggtcatt tacgccatcg
     5341 gtcacattac cgctgttcac tggcggaagt aatctggcgc agcttcgtta cgcagaagca
     5401 caaaagcgtg gactcattgc cacctatgaa aaaaccgttc agagcgcatt taaagatgtc
     5461 gctaacgcac tggcgcggcg gaccacgctt gaagagcaac tggatgcaca gcgccagtat
     5521 gttaaagctg agcaacaaac ggtcgatgtt ggcttgcgtc gctatcagac gggtgttggt
     5581 gattacctga ccgtgttgac ggcgcagcgc agcttgtgga gcgcacagca ggagctactg
     5641 gcactgcaac tgactgattt tacaaaccga atcacgctct ggcagtcgct gggcggcggc
     5701 atgtcatcac tcaagtaagg aataatatgg ctagagtctc tctttcatgg gcattgattc
     5761 ttggtctttt agccggtatc ggcccgatgt gtaccgatct ttatttgccg gctttgccgg
     5821 agatgtctta gcaactggcg gcaaccacga ccataacgca attaacgctg actgcatcac
     5881 tgatcggtct tggcgtcgga caactgttat ttggccctct gagcgacaaa atagggcgta
     5941 aacgtccgtt aatcttgtcg ttgctattgt ttattgtttc ttccattttg tgcgcgacaa
     6001 cgaacaatat ttactggctg gtggtctggc gttttattca agggatcgcg ggggcgggtg
     6061 gttcggtgct ctctcgttct attgctcgtg acaaatatca gggagtaacg ttgacccagt
     6121 tttttgcgct gttaatgacg gtgaatggcc tggcaccggt gttgtcgcca gtgctgggcg
     6181 ggtacattgt tagcactttt gactggcgca ctttattctg ggtaatggct gaaattagcg
     6241 ccgtattgtt gctgggctgc gtgttattta ttaatgagac cttgccagaa aataaaaggg
     6301 gctcatcatt gctattaacc ggacgaagcg tggtgcagaa ccgccgtttt atgcgctttt
     6361 gcctgattca aagttttatg ctggcaggct tgtttgcgta tatcggctct tcgtcgttcg
     6421 tgttgcagaa ggaatttggc tttagtccaa tgcaatttag cctggtgttt ggccttaacg
     6481 gcatcggact tatcattgct tcatggatct tttcgcgtct ggcgcgacgg attaatgcga
     6541 tgacattgtt gcgaggtggc ctgatagcgg caattttgtg tgcattgctc acggtcttat
     6601 gcgcatggac acaattgccc attccggcac tggtggcatt atttttcact attgcatttt
     6661 gtagcggcat cggcactgtt ggcggggcag aggctatgag tgcagtaggg acacaggaat
     6721 ctggaacggc gtctgcgttg atggggatga gcatgtttgt cttcggcggt atagccgcgc
     6781 cattgtcggg atttggcgga gaaacactgt taaaaatgag tctggcaatt acggtgtgtt
     6841 atacactggc attcctggtt gctctcacca gaatcgacaa tcaaaagtaa tgaatacctc
     6901 atcctgaggg ggggcccctg tcaggatgag gtgcagaaca ctaacggtcc ttacgccacg
     6961 catccgctgt caatgcctcg ccaaaatgac cggcaatcag ccgtttggtg agttcatgca
     7021 gcggcgatgc cagcacatcc gcggtgctgc ctcgctcgac aacctcgccc tgatgcatca
     7081 ccagcaccag gtcgctaatg tgcttcatca ttccgatatg ctgagtaaca taaatatacg
     7141 aaatgccctg tttttcctgt aattccagca tcagattaat caactgcgaa cgcatcgaca
     7201 tatccagtga agcgagggct tcatcggcaa taatgacttt tgggcgcaat atcagcgcgc
     7261 gcgccagacc cagacgctgt ttttgcccgg gggccaacat atgcggatag taactgacgt
     7321 gatccggtag cagcccaacc atacgcatcg tttcaataat ctgtttgcga cgctgttccg
     7381 gttccaggtt ggtgttcagg cgcagtggaa aatccaggat ttgcgagata cgttgacggg
     7441 gattcaacga ggtcgaagga tcctgaaaaa tcatgcgaat acgctgactg cggaaggaat
     7501 aatcgccaaa atgcagtgga tgatcgtcaa tcaataactc gccgctggta ggttctatca
     7561 ttcccgccag catttttgcc agcgtggatt tacccgaacc attctcgcca ataatcgcca
     7621 gtgtctggcc ttcacgtagc gtaaagctca agggttttac cgcttctacg gtctgacgac
     7681 gaaaccaacc ggtccggtag cggaacgttt tacttaaatt acgcacttca agcagcgttt
     7741 cgatcatctc actctttctc catgttcagc gggaaatgac aggcatagag atgatttttc
     7801 gcccccgtca aacgtggcgt cacaatgcat tctcgttgtg cgtacgggca acgcggcccc
     7861 agacgacaac caatcggtaa ctgttccagc agtgggatag cgccgggcag cgtattgagg
     7921 cgacttttat gcggcatcgc gctgccgaag tctggtatcg cgcggatcag cgcctgggta
     7981 taaggatgat gtggcatcgt caccaactcc ttactcggcg cggtttccac cgtttgaccg
     8041 cagtaaagca cgttaatttt atccgcccat tggctaagca tttgtaagtc atggctgata
     8101 agcaaaatag tggtattgct gttttggttg agacgcgtca gcaggcgaaa gatttgcgcc
     8161 tgggttgttg gctccattga gttggtcggt tcgtcagcaa tcagcagacg cggttgattc
     8221 gccagtgcaa tggctatcat cactttctga cattcacctt cggtcaactc atagggaaaa
     8281 ctgcgcatcg catctttgtg atctttaatc cccacgcggt gcagcagttc aatggcacgc
     8341 cgtttgcgcc agccaaaacg ctgccaccaa cggcctttat aggtccaggc tgggatgttt
     8401 tgcatcaact ggcgacccac acgttctgaa ggatctagac acgactgcgg ttcctggaaa
     8461 atcatcgaca cgttgtggcc aaccagcttg cgccgttcgc gtgcggagag acgcagcaaa
     8521 tcgatatcat caaaacgcat acggtctgca gtaacacgcc agttatcttt attcacccca
     8581 caaatcgctt tcgcaatcaa acttttgccg gaaccggatt cgccaacaag accgcggatt
     8641 tcaccttcgg ttaaggtcat acttacccgg tcaacggctt taacccactc atcaccggtt
     8701 ttaaattcaa tagtcaggtt acgaatatcg agtaatggca ttattccacc cccgcaataa
     8761 ttgcacgacg gacaccgtcg ccaaggaggt taaccaacag cacgctaatc ataattgccg
     8821 cacctggcag catgacagtc cacggggcga cataaatcag ttccagcgca tcaccgagca
     8881 tcgctcccca ttcaggcgag gggagttgtg cgccaagatc gagaaagccc agcgcggcga
     8941 tatcgagaat tgccatcgac agtgcgcggg tgatctcggt gaccagcccg gcggtgatgt
     9001 taggcatcac agcaaaccag agaatattca gcgttgatgc gccatccaga cgggcggcga
     9061 taacgtactc tttttccagt tcgtcatgca ccatgctgta aatcgaacgt accatacgcg
     9121 gcagcaatgc cagccagaca gcaaacatag cgtgggacaa actcggtccg gcaaacgcta
     9181 caacgataat tgccaacagc agggaaggga tcgccagtaa ggtatccaga atatggttaa
     9241 gcaccgctga gcggaggccg tgcgtcgccc cggcgaaggt gcccagtacc aggccacaga
     9301 tcgtcgcggc aagtgtcacc acaaatgcgc cacccacggt tggcgcagct ccgctcagta
     9361 aacggcttaa aacatcgcgt ccgaggtcgt cagtccccag gaagaaagaa acttcgccat
     9421 agcgcgacca tgatggcggc aataattggt aaccgagaaa ttgctggtcg atgccgtaag
     9481 gcgcaaacca gccgccaaaa atacacagta cagccagccc cgcgcagccg tacaggccga
     9541 ccatcgcaga ggcgtcacta taaaatttgc gccaggcggt acgcagcgtg cccggcgggc
     9601 gcttttcgct gtatacgcta tcgtaaggca taccattcct tatgtttcag agggttagcc
     9661 atggcaccca aaatatcaga aatcacgtta acaataatga ccagtgagcc acacaccatc
     9721 actccggcgg aaatggctgc gtaatcctgc tggcggatgg cgttaattaa ccagcgccct
     9781 aaacccggcc agctaaaaac catttcggtg atcatcgcca gcgtcagcat ggtggaaaac
     9841 tgtaagccca ggcgaggaat aaccggcggt aacgcgttat gcagaacgtg gcgacgcaaa
     9901 atagtaaagc gcgacaaacc gcgggtcgcc gccgctttta catagttctg gtcatacact
     9961 tcgatagtgc tgatacgcat caggcggatc acttcagttg ttggcgcaac cgacagggtg
    10021 atcacgggca atatcatatg gcggattgcg ctcatgatca tttcatcccg ccatggtgag
    10081 tccgaaagcc aggcatcaat caacgcaaaa ccggtaatcg gtttcacttc gtagagcaga
    10141 tcgaaacgcc ctgaaacggg caaccaacct agcgtgagtg aacaaaacag tgtcaacaga
    10201 agcgccagcc agaacaccgg gattgaaaag cccagtaagg cgatggcgtt gatcaagtta
    10261 tcctgccact tatggcgcgt aatcccggca atcatcccca cggggatgcc aacaatcagc
    10321 gcaaaaccga aggcgaggat gcacagttcc atcgtcgccg ggaagacctc ttttaactgt
    10381 tcggcaattg gttgaccgtt aatgctggaa acaccaaaat cccagtggat caggccatta
    10441 aaccagaaca cccaggcatt ccacagtgac gcgccttgta acggcgcgtg tggagtgaaa
    10501 tagctcaagc tgaagccaac gaaggtcagc aggaacaagg tgacaatcaa taacaaaatg
    10561 cggcgtaagg tgaagataat catggttttt tcacctcatc ctgtttctcg cgatacaccc
    10621 cagcaaagga ggcgttacca aacgggctaa gcaccagacc tttgatatcg taccgatagg
    10681 cctgcaaacg caatgacgac gccagcggca aaatgggcaa ttcctgcgcc agaatactct
    10741 gcgcttcgtc ataggcttca atacgcgccg ccagctgctg cgaggagagc gccttacgca
    10801 acacgctgtc gaatttcgga tcgcaccagt gggcgaggtt ggtctgtgaa tgaattgccg
    10861 cgcagctcag taacggacgg aagaaactgt ccgggtcgtt actgtccgtc gcccaaccgg
    10921 ataacgtcag atcatggctc atatccatca accgcgcctc ctgaaaccga ccttctaccg
    10981 gcacaatcac cacttttacg ccaacctgcg ccatatccgc ctgaatcagt tcggcagttt
    11041 tcagtggact ggggttccac gcctgcgaac gtgtgggcac ccacagtttc agcgttaaat
    11101 tttccagccc caacgacttc aactgttcgc gcgatttcgc cggattgtat tcagtaattt
    11161 tagcctcgtt gtcataggcc cacgaggcgc gcggtaaaat agaggccgcc gtttcagccg
    11221 taccataata gatggattgc atcaggcgct ggttattaat cgccagtgcc agcgcatggc
    11281 ggacagcggg attatttagc ggcggtttgg cggtgttaaa tgccagatag gcgacgttca
    11341 tcccaggacg cagcgttaaa cgcaagcgcg ggtcgtcacg caaaatggat agctggctgg
    11401 cagcaggcca ggccagaacg tcgcattccc cggtcaggag tttcgacaga cgtccggtgc
    11461 cgccggagcc taaatccacc accacctgcg gcattaacgg tttaccgcgc cagaagtcat
    11521 catgacgttg taggcgaata aattgcccgg cgcggtattc cgacaactga tacggtccag
    11581 tgccgaccgg ttgacggtcg agttgctcct ggcgatcttc tttctctaac ttccgggcat
    11641 attctgccga catgaccgaa gcataatggg ttgcgaggtg ccacaaaaaa gaagcatccg
    11701 gctgagccag acggaactca acggtatgat tatccagctt gcggacgctt ttaacgttat
    11761 cggcaaattg cagactgtcg aaatagggga agttactacc gttgacgtta tgccacgggt
    11821 tattgcggtc aaaaatccgt tggaaggtaa acaccacatc atcggcattc attttgcgag
    11881 tgggagtaaa ccaggcggtt ttttgaaacg ggacatcgcg acgcaggtgg aagcgataag
    11941 tcgcgccgtt gtcgagcact tcccagcttt cggcaagttc tggcatcaga cgataggtat
    12001 acggatcgac atccagcagt cgatcataaa actgggcggc aagggtatcg acaattaacc
    12061 cactgctcgc tttggatggg ttaaaggtgt tgacttgccc gctgacgcaa tagacaaaac
    12121 cgctgtcgcg gatatcagca tgcggggtgg gttcaggcgc ggcgattgcc tgaccattca
    12181 caagtccagc aatcaccaaa agagacgata atacctggcg cataatatta agggatttta
    12241 tgtaaagagg ctatcttact aatatttaat gacatttgcc attaccgttt gtgttcaggg
    12301 ggcgtaatga gggccgtgtg gttggtcgtt gaacaggtgg cgtcgccatc tgttcaacat
    12361 tcgtacccgt gatttctcta taactataac tcacagaata acttagcgag gaacggagag
    12421 gtacgatcgt gagaaaccca ttaaagcagg ctgtagtgcg tcatttttag ccggatgcgg
    12481 cgtgaacgct ttatccgggc aacgatactg accaggcgcc tgcaatgata taattactca
    12541 ttaccccatt ggttcaaaaa ctctgcgatt tcatcaatgc gcgcggaatt aatccccgct
    12601 tcagcggcca tgtcatgctg ggcttcttcg ctgatctctt cattgttcat caaacgggtg
    12661 agtagtaact ggaagtagtg ggccagcggt gtgcgttctg cgtctgcgac aggttctgcg
    12721 cattcagtcg cgtattcatc ggcaatatca aaatatttta atgggatatc gtggttcatt
    12781 gtttgcccct ggggttaatg cgctacgcat aggggcggat aatagcactc gtgatccggg
    12841 tcatacatct ttttatagct aatagagcgg cgcattaccc tcaggtcgga taatgcgccc
    12901 gcatccaaca attacgtctc gctcaccggc gttctgtcac cctcgtacag cggtactttg
    12961 cgataacgac gtaccagata ccacagatac gccccaccca gcgaggccca caccagaccc
    13021 agcgtcagcg aggtggcttc gaggttaatc cacagtacgc ccaccgtcag cgcgccaacc
    13081 agcggcatta ataaatagtg gaagtgttcc ttccaacttt tattcattcc tttacgccgc
    13141 cagaaatgat taaacaccga cagattcacg aaggtaaacg ccaccagagc accgaagtta
    13201 atcaatgctg tcgcggtgac taaatcaaag aacaatgccg acatcgcgac aataccgacc
    13261 ataatgacgt tcagagccgg ggtacgccat ttcgggtgta catagccaaa cacacgttct
    13321 gggaacacat tgtcacgccc cattacatac agcaggcgcg acacgctggc atgtgaagcc
    13381 aggccagagg ccaatgtgtt cacaaatgtg gtgcagagga aaatagactg gaacagcttg
    13441 ccgccgacgt acagcgcaat ttcaggcagt gcagcgtccg gatctttaaa gcggctgata
    13501 tcgggaaaga acagctgcat aaagaacgac gccgcgataa agataacgcc accgtagacc
    13561 gccgtcagga agatggcttt cgggatcacg cgtgcggcgt ccggcgtctc ttccgaaagc
    13621 gtggtcaccg catcgaaacc gaggaacgag aagcacacaa tcgtcgcccc ggtaataatc
    13681 gggatcaggt gcgcgttctc gctgataaac ggttgaagtg accagacggt gccaacgcct
    13741 tctcctttat gcagtccctg aaccaccagg aagataaaca ccaccatgat ggagatttgc
    13801 accagcacaa acagggtatt gaagttggcg accaggttaa cgctcttcag gttcgcggca
    13861 gttaaaatgg cgacgaaggt caccacccac acccacggcg gcacttccgg gaagagggcg
    13921 gagagataga tttttgccaa cagtacgttg atcatcggca aaaagagata atccagcagt
    13981 gatgaccagc cgaccataaa tccgacgtgc ggattaatcg acttttgcgc gtaggtatag
    14041 gccgaaccgg cctccggaaa ctggcgaacc agtttgccgt agctgatagc ggtaaacagc
    14101 acgcccgcca gcgccagcaa ataggacgcc ggaacgtgac cgtcgctaat gcctgacaca
    14161 atgccaaaag tatcgaacac agtcatcggc gtgagatagg ccagacccat cattaccacc
    14221 tgccacaatt tcagtgattt tcgcagacgg gttttgccgg gttgcgcagc aatattcagt
    14281 ggtgaattaa tagccataat tgctgcctcc cctgcgtaat aaggtttgcg gtcgtgtcga
    14341 cctgaaatca aacctgttta gcaagggaag caaagggggc ggagtccagt cagtagcagg
    14401 aggggcgcat acgcagcctc cctcgccatg cggggattta atcatcggca tcatctcatt
    14461 tcctcgtcac agtcttaatc gttaacggaa gcccgttgcc gcctgcgctc cataacgcga
    14521 catccgggaa atgtctggat taccagcaaa tgtcaggcct ggctccgctc aggccgatga
    14581 aacaaccccg caaggggtat tacgcgtttt tcaacatcca ctcaatttct gtttctgtga
    14641 tgaggcgctc aaactgcaac agctcatcat ttttacaggc gtgatagaca tggcaaaacc
    14701 gttcgcctaa atagcggcgc aggtgatcat tctcgataaa ctcacccagg gcatcgctct
    14761 ggcgaatcgg gaagggtaag ccttcctgtt ccagcccgtt accttcgact tcttcctgca
    14821 acggcagctc gttatcaagg ccatgcaaaa taccggcaaa aatcgctgcc atcaccagat
    14881 atgggttggc atcggcaccc gccacgcgat attccacgcg gtgattatgg cgatcgccgc
    14941 atggaatacg cagggcgacg gtgcggttgt tatggcccca cgacgcctgc gtcggtacat
    15001 acatccccgg ctggaagcgg cgatacgagt tcacgtttgg tgccagcaac gccattgatg
    15061 acggcatcag atcgatcatt ccggcgagca tttttttcag cagcggcgaa tcttcgcctt
    15121 ccgcgtcaga aagcacgttc tcgccacgat tattttgcat actgatatgg atatgcattc
    15181 cgctgcccgc gtgctcttca tacggcttcg ccataaaagt ggcgtgcatc ttatgctttt
    15241 ctgccatcag acgcaccagg cgttttagtg ccagcgcatc atcgcaggct tccagcacgt
    15301 tatcagtatg gtaaaggttg atttcaaact gacccggcga ggcctcagcg accgcgccat
    15361 ctgccggaat cagctgtaac tgcgccagtt catcaatatc attgagcacg tcggcgaagt
    15421 ggttgagatt atcaacggag taaacctggc tttgtgtatt gcggtcatcg gtgccgggcg
    15481 cgcagggcgg ttgcagatac ccttcagcgt cgcgctggcg atccagtaaa tagaactcca
    15541 gctctaccgc tacgaccggg aacaatccgc gctggcgcag ctgctgccac aggcggttaa
    15601 gaacgttccg cggctcaacg tcaaagggag cgccatcttc atcgaccatg gtcaggagca
    15661 tctgaccgat aaactctgga tcggcggcag aaggggttaa ggaaccgaga acaggaacac
    15721 aggtacgatc cggctcgccc atttcctgac ccagacccgc ttcttctact acgttgccca
    15781 gaatatccat tgcaaacacc gaggccggga aataacaccc tttctccagc ttcttcaggc
    15841 ttgaaacagg aatacgtttt ccacggaagc aaccattcaa atcggtaagt aaaacatcaa
    15901 catattgcgt attcgggtag cgctccaggc agcgtttcac ttcctgcgta aaggcgctac
    15961 cccgcctctc ttctgactgc tgaacaaagt tctctacttc aacgatattg gtttccatga
    16021 ttcttcgcct ttggtttgtt ttccgctcgt tatcaaagcg taaaatataa tgaccaccat
    16081 tcgaatctgt atgcaaacta aatgtttgtc aaatgttaaa ttgagtttgc aaaaatgaaa
    16141 atccactgct ggattgaaaa aatattgaac ataaaggtca tttaaaccgc agtaatggcg
    16201 ataatttagt ccactttgtg agattgagca tggaaaatat aatgaacaat ccggttatcg
    16261 gtgtcgtaat gtgcaggaac aggcttaagg gtcatgcgac ccagactctg caagaaaagt
    16321 acctgaatgc catcatccat gcaggcggct tgccaattgc gctgccacat gcgctggcgg
    16381 aaccgtcgtt acttgaacaa cttttgccga aactcgatgg catttacctt cctggtagtc
    16441 ccagcaatgt gcagccgcac ctatatggtg aaaacggcga tgagcctgac gccgatcccg
    16501 ggcgtgatct tctgagcatg gcgttaatta atgccgcact cgaaaggcgc atccccattt
    16561 tcgccatctg ccgggggtta caggaactgg tggtggcaac cggtggttcg ttgcatcgca
    16621 agctgtgcga acagcctgaa ttgctggaac atcgggaaga tcccgaactg ccggtggaac
    16681 agcaatatgc accgtcgcat gaagttcagg ttgaagaggg gggattactg tctgcgttgt
    16741 tacctgaatg tagcaatttt tgggtaaact ctctgcatgg acaaggggcg aaggtcgtta
    16801 gcccacggtt gcgtgttgaa gctcgctcgc cggatggctt agttgaggcg gttagcgtca
    16861 tcaatcatcc ttttgcgctg ggcgtacagt ggcacccgga atggaacagt agcgagtacg
    16921 cgctttcgcg tatattgttc gagggcttta tcaccgcttg tcagcaccat atcgctgaaa
    16981 aacagcgact ctgaccacta cagtttaagg aaatgcaaat atgagtgatg agggactggc
    17041 gccaggaaaa cgcttgtcgg aaatccgcca gcagcagggg ctttcacaac gtcgtgccgc
    17101 cgaactctcc gggctgactc acagtgctat cagtacgata gaacaagata aagtcagccc
    17161 tgccatcagt acgctgcaaa agctgctgaa ggtgtatggt ctgtcactct cggaattctt
    17221 ttccgagccg gaaaaacctg atgagccgca ggtcgtcatt aatcaggacg atttaattga
    17281 gatgggcagt cagggtgtgt caatgaagct ggttcataac ggtaacccga atcgcacgct
    17341 ggcgatgatc tttgaaacgt accagccggg cacaaccacc ggggaaagaa ttaagcatca
    17401 gggtgaggaa ataggcactg tactggaagg tgaaattgtt ctgacgatta atggtcagga
    17461 ttaccacctc gtcgcggggc aaagttatgc cattaatacc ggcatcccgc acagtttcag
    17521 taatacgtcg gcaggtattt gccgaattat cagcgcccat acgcccacca cgttttaatc
    17581 tttttgttct gtaagccggg taagcgcaat gcgcccggca atccataaag aatgtcgcca
    17641 gaaataaacg ggcatacggc ccggggatct ctgcgccctg acgttcacaa actgcatata
    17701 tctgatagac gtgaaacagg agtcataatg aattttcatc atctggctta ctggcaggat
    17761 aaagcgttaa gtctcgccat tgaaacccgc ttatttatta acggtgaata tactgctgcg
    17821 gcggaaaatg aaacctttga aaccgttgat ccggtcaccc aggcaccgct ggcgaaaatt
    17881 gcccgcggca agagcgtcga tatcgaccgt gcggtgagcg cagcacgcag tgtatttgaa
    17941 cgcggcgact ggtcgcaggc tgccccggca aaacgtaaag cggtgctgaa taaactcgcc
    18001 gatttaatgg aagcccacgc cgaagaactg gcgttgctgg aaaccctcga caccggcaaa
    18061 ccgattcgtc acagtctgcg tgatgatatt cccggcgcgg ctcgcgccat tcgctggtac
    18121 gccgaagcga tcgacaaagt gtatggcgaa gtggcgacca ccagtagcca tgagctggcg
    18181 atgatcgtgc gtgaaccggt cggcgtgatt gccgccattg taccgtggaa cttcccgctg
    18241 ctgctgacct gctggaaact cggcccggcg ctggcggcag gtaacagcgt tgtcctgaaa
    18301 ccgtctgaaa aatcaccgct cagtgcgatt cgtctcgccg ggctggcgaa agaagcaggt
    18361 ctgccggatg gcgtactgaa cgtagtgact gggtttggtc atgaagcggg gcaggcgctt
    18421 tcgcgtcata acgatatcga cgccattgcc tttaccggat cgacccgtac cgggaaacag
    18481 ctgctgaaag atgcgggcga cagcaacatg aaacgcgtct ggctggaagc gggaggcaaa
    18541 agcgccaaca tcgttttcgc cgattgttcg gatctgcaaa aagcggcgag cgccaccgca
    18601 gcaggcatct tctacaatca gggtcaggtg tgcatcgccg gaacgcgcct gttgctggaa
    18661 gagagcatag ccgatgaatt cttagcgctg ttaaaacagc aggcgcaaaa ctggcagccg
    18721 ggccatccac ttgatcccgc aaccaccatg ggcaccttaa tcgactgcgc ccacgccgac
    18781 tcggtccata gctttattcg ggaaggcgaa agcaaagggc aactgttgtt ggatggccgt
    18841 aacgccgggc tggctgccgc catcggcccg accatctttg tggatgtaga cccgaatgcg
    18901 tccttaagcc gcgaagagat tttcggtccg gtgctggtgg tcacgcgttt cacatcagaa
    18961 gaacaggcgc tacagcttgc caacgacagc cagtacggcc ttggcgcggc ggtatggacg
    19021 cgcgacctct cccgcgcgca ccgcatgagt cgccgcctga aagccggttc cgtcttcgtc
    19081 aataactaca acgacggcga tatgaccgtg ccgtttggcg gctataagca gagcggcaac
    19141 ggtcgcgaca aatctctgca tgcccttgaa aaattcaccg aactgaaaac catctggata
    19201 agcctggagg cctgaaatga ccgaacatac cagcagttat tacgccgcca gtgcgaataa
    19261 atacgcgcca ttcgacacgc tgaatgagtc gatttcctgc gacgtttgcg tggtcggcgg
    19321 cggctatacc gggctttcct ccgcgctgca tctcgcagaa gcgggctttg acgtggtggt
    19381 tcttgaagcc tcacgcatcg gctttggcgc cagcggacgc aacggcgggc agcttgtgaa
    19441 ctcctacagc cgcgacatcg acgtgatcga aaaaagctac ggcatggaca tcgcccgtat
    19501 gctcggcagc atgatgttcg aaggtggtga gatcatccgc gaacgtatca aacgttatca
    19561 gattgactgc gactaccgcc ccggcggtct gtttgtggcg atgaatgata aacagctcgc
    19621 cacacttgaa gagcagaaag agaactggga acgctacggc aataaacagc tggaattgct
    19681 ggacgccaac gccattcgcc gtgaagtagc cagcgatcgc tacaccggcg cgctgctgga
    19741 tcacagcggt ggacatattc acccgctaaa cctcgccatt ggtgaagccg atgccattcg
    19801 tctcaacggc gggcgcgtgt atgagctttc tgcggtgacg cagatccagc acaccacgcc
    19861 agcggtggtg cgcactgcca aaggtcaggt gacggcgaag tatgtgattg tcgccgggaa
    19921 tgcgtacctg ggcgataaag tggagccgga gctggcgaaa cgcagcatgc cgtgcggtac
    19981 gcaggtgatc accaccgagc ggctttccga tgatttagcc cgttcgctga tcccgaaaaa
    20041 ctactgcgtg gaagactgta attatctgct tgattactac cgtcttaccg ccgacaaccg
    20101 tttgctgtac ggcggcggcg tggtgtacgg cgcgcgcgac ccggatgacg ttgagcgtct
    20161 tgtggtgccg aaactgctga aaaccttccc gcagttgaag ggcgtgaaaa tcgactatcg
    20221 ctggacgggc aacttcctgc tgaccctgtc gcgtatgccg cagttcggtc gcctcgatac
    20281 caacatctat tacatgcagg gttacagcgg ccacggcgtc acctgtaccc acctcgccgg
    20341 gcgtctgatt gccgaactgc tgcgcggcga cgccgaacgt ttcgatgcct tcgccaatct
    20401 gccgcattac ccgttccccg gcgggcgcac gctgcgtgtg ccgtttaccg cgatgggcgc
    20461 ggcctattac agcctgcgcg atcgtctggg tgtttaattt ccgattaaca gtgaagagtc
    20521 aaaaggtgtg aaacatgagc aacaatgaat tccatcagcg tcgtctttct gccaccccgc
    20581 gcggggttgg cgtgatgtgt aacttcttcg cccagtcggc agaaaacgcc acgcttaagg
    20641 atgttgaggg caacgagtac atcgatttcg ccgcaggcat tgcggtgctg aacacggggc
    20701 atcgccaccc tgaattggtc gcggcggtgg agcagcaact gcaacagttt acccacaccg
    20761 cgtaccagat tgtgccgtat gaaagctacg tcaccctggc ggagaaaatc aacgcccttg
    20821 ccccggtgag cgggcaggct aaaactgcgt tcttcaccac cggcgcggaa gcggtggaaa
    20881 acgcggtgaa aatcgcccgc gcccataccg gacgccttgg cgtgattgcg tttagcggcg
    20941 gctttcacgg tcgtacgtat atgactatgg cgttaactgg aaaggtcgcg ccgtacaaaa
    21001 tcggcttcgg cccgttccct ggttcggtgt atcacgtacc ttatccgtca gatttacacg
    21061 gcatttcaac acaggactcc ctcgacgcca tcgaacgctt gtttaaatcg gacattgaag
    21121 cgaagcaggt ggcggcgatt attttcgaac cggtgcaggg cgagggcggt ttcaacgttg
    21181 cacccaaaga gctggttgcc gccattcgcc gcctgtgcga cgagcacggt attgtgatga
    21241 ttgctgatga agtgcaaagc ggctttgcgc gtaccggtaa gctgtttgcc atggatcatt
    21301 acgccgataa gccggattta atgacgatgg cgaaaagcct cgcgggcggg atgccgcttt
    21361 cgggcgtggt cggtaacgcg aatattatgg acgcacccgc gccgggcggg cttggcggca
    21421 cctatgccgg taacccgctg gcggtggctg ccgcgcacgc ggtgctcaac attatcgaca
    21481 aagaatcact ctgcgaacgc gcgaatcaac tgggccagcg tctgacaaac acgctgattg
    21541 atgccaaaga aagcgttccg gccatcgcgg cggtacgcgg tctggggtct atgattgcgg
    21601 cagagtttaa cgatccgcaa acgggcgagc cgtcagcggc gattgcacag aaaatccagc
    21661 aacgcgcgct ggcgcagggg ctgcttctgc tgacctgtgg cgcatacggc aacgtgattc
    21721 gtttcctgta tccgctgacc atcccggatg cgcaattcga tgcggcaatg aaaattttgc
    21781 aggatgcgct gagcgattaa tagcccaacg caataatgtc tgatgcgctg cgcttatcag
    21841 gcctgcaaac gacgtattga ttatgtatgc cggataaagc attcacgccg catccggcaa
    21901 gttgtattgc ccaacttcgc taaatctggt gctttttcaa caacgcgcgg aactgatgat
    21961 aggttaaccc cagtaattca gccgcacgct tctggttata tttcccttgt tgcaaactga
    22021 gttgcagcaa ctctttttcc tgctgcatct gaaactcacg taaatccagc ggcagtgttg
    22081 gaagcgaggt ggtttctgaa acggcgatag cgtcttcagg cggacgccgt ttaaagggat
    22141 caataatgat gtcatcaagc ggataatcgc tggtgccgtg gcgatacact gaacgttcca
    22201 ccacgttttt caattcacga atatttcccg gccaacgata attcagcaat gtttctctgg
    22261 cgtgctcggt aaaacccggg aacagaggca gcttgatttc ccgacacatc tggatggcaa
    22321 agtgttccgc catcaacata atgtcgcttt cgcgctcgcg cagtggtggc agttgcacaa
    22381 catcaaaagc caggcggtcg agcaggtcag cgcgaaaagt accttcattg accatcgccg
    22441 ggagatcggc attcgtcgcg cataccaacc gcacattcac ctgcaacggc tggcagccgc
    22501 caacgcgctc cagttcaccg tactcaatca cgcgcaataa tttttcctgc accatcatcg
    22561 gtgctgtagc gagttcatca agaaatagcg taccgccgtc ggcacgttca aatctccctg
    22621 gatgacgttt ttgcgcaccg gtaaacgccc ccgcttcgtg accaaacagt tcggaatcca
    22681 gcagattttc atttaacgcc gcgcagttaa gggaaataaa cggcccttgc caacgggagg
    22741 agagataatg caggcggctg gcgatcagct ctttaccggt gccgcgttcg ccgatgatga
    22801 gcaccggttt gtccagcggc gcgagatgcg aaacctgttc cagcacttcg agaaagctgt
    22861 tcgcctcacc gagtaaatta tctttgtatt ctgccatgat gaaattcgcc acttgttagt
    22921 gtaattcgct aactcatcct ggcatgttgc tgttgattct tcaatcagat ctttataaat
    22981 caaaaagata aaaaattggc acgcaaattg tattaacagt tcagcaggac aatcctgaac
    23041 gcagaaatca agaggacaac attatgggta ttttttctcg ctttgccgac atcgtgaatg
    23101 ccaacatcaa cgctctgtta gagaaagcgg aagatccaca gaaactggtg cgtctgatga
    23161 tccaggagat ggaagataca ttggttgaag tacgttctac ttcggcgcgt gcgctggcag
    23221 aaaagaaaca gctgactcgc cgtattgaac aagcgtcggc gcgtgaagtt gaatggcagg
    23281 aaaaagccga actggcgctg ctgaaagaga gagaggattt ggcgcgtgcg gcgttaattg
    23341 aaaaacagaa actgaccgat ctgattaagt ccctggaaca tgaagtgacg ctggtggacg
    23401 atacgctggc acgcatgaag aaagagatcg gtgagctgga aaacaaattg agcgaaacac
    23461 gcgctcgcca gcaggcattg atgttacgcc atcaggcggc aaactcgtcg cgcgatgtgc
    23521 gtcgtcagct ggacagtggc aaactggatg aagcaatggc tcgtttcgaa tctttcgaac
    23581 gtcgaattga ccagatggaa gcggaagcag aaagccacag cttcggtaaa caaaaatcgc
    23641 tggacgatca gtttgccgaa ctgaaagccg atgatgcaat aagcgagcaa ctggcacaat
    23701 taaaagccaa aatgaagcaa gacaatcaat aataacatcc aggcggcgtc cgaaagcgcc
    23761 gccgctcatc gtctaaggag tacttatgag cgcgctattt ctggctattc cattaaccat
    23821 ttttgtgctg tttgttttac cgatctggtt atggctgcat tacagcaatc gttctggtcg
    23881 cagtgaattg tcgcaaagtg agcagcagcg attagcgcaa ctggctgatg aagcaaaacg
    23941 gatgcgcgaa cgtattcagg cgctggaatc tattcttgat gcagaacatc cgaactggag
    24001 ggatcgctaa tggcgggcat taatctcaat aaaaaattat ggcgtattcc acagcagggc
    24061 atggtccgtg gcgtctgcgc cgggattgcc aactattttg atgtaccggt aaaactggtg
    24121 cgtatcctgg tggtgctgtc gattttcttc ggtctggcgc tgtttaccct ggttgcttac
    24181 atcattttgt catttgcgct tgatccaatg ccggacaaca tggcctttgg tgagcagcta
    24241 ccttccagca gtgaattgct ggatgaagtc gaccgtgaac tggcggcaag tgaaacgcgt
    24301 ttacgcgaga tggaacgtta tgtcacttcc gatacattca cgttacgtag ccgtttccgt
    24361 caactgtgag gaaagttatg aatactcgct ggcaacaggc cgggcaaaag gtgaagcctg
    24421 gtttcaaatt agcaggcaag ctggtacttc ttaccgcact gcgctatggc ccggcgggtg
    24481 tggcgggctg ggcgataaaa tcagttgctc gccgaccgct gaaaatgttg ctggctgtgg
    24541 cgctggaacc gctgttaagt cgggctgcca ataaactggc acagcgttat aaaaggtgag
    24601 gggagtttcg caaaaattgt taaatctcag gcgtataatg gatggcaatt ttcatccata
    24661 gaaggacgct tacatgttta aaaaaggctt acttgctctg gcactggtgt tttcactgcc
    24721 cgttttcgcc gctgaacact ggatcgatgt tcgtgttcca gagcagtatc agcaggagca
    24781 cgttcagggg gccatcaata ttcccctgaa ggaagtgaaa gaacgcattg ccaccgccgt
    24841 tccggataaa aacgacaccg tgaaagtgta ttgcaatgcc ggacgccagt ccgggcaggc
    24901 aaaagagatc cttagcgaga tgggatatac ccacgttgag aacgccggtg gcctgaaaga
    24961 catcgcaatg ccgaaggtca aaggttaaaa gaattcacca tgagcggcgc ttatgcgccg
    25021 tttttttctg tcacacctta tttacatccc tatagattat ttgcgtcagc tcacaaatac
    25081 gttttttccc tggtaaaaaa tgatttcctg cgtgactaaa acccttgtgc tcaattgaca
    25141 gtttattttc tgcggagtag tctctcgttt catgggaccg ctaccacgga aaggcaacat
    25201 gaaacagaaa attacggatt acctgggcga aatctacggt ggaacattta ccgcaactca
    25261 tttacagaaa cttgtaacgc gtcttgagag tgcgaaacga ttaattacac agcgacgtaa
    25321 aaaacactgg gatgaaagtg acgtcgtgtt aattacctat gccgatcaat ttcacagtaa
    25381 tgatttaaaa ccactaccca catttaatca gttttaccac caatggctgc aaagcatttt
    25441 ttcacatgtt catttattac ctttttatcc atggtcatct gatgatggat tttcggtaat
    25501 tgattatcat caggtcgcca gtgaagcggg ggagtggcag gatattcagc agctcggtga
    25561 atgcagtcat ttaatgtttg attttgtctg caaccatatg tcggcaaaaa gtgaatggtt
    25621 taaaaactat ttgcaacagc ttccgggttt taaagatttc tttattgcgg ttgatccgaa
    25681 aaccgatctc agagccgtca cccgtccgcg tgcattaccg ctattaacgc cgtttcaaat
    25741 ggatgataat tccacgcgcc atttatggac cacctttagc gacgatcaaa ttgacctcaa
    25801 ttaccgtagc cctgaagtgt tgctggcgat ggtggatgta ttgctgtgct acctggaaaa
    25861 gggcgcagaa tatgtccgtc tggatgccgt tggctttatg tggaaagagc cgggaacaag
    25921 ctgcatccat ctggaaaaaa cacatctgat tatcaaactg ttacggtcga ttattgatga
    25981 catcgcgcca ggtacggtga ttattactga gacgaatgtt ccgcataaag ataacattgc
    26041 ttactttggc gaaggcgatg acgaagcgca tatggtgtac cagttctcgc tgccgccgct
    26101 ggtgttacat gcggtgcaaa aacagaacgt cgaggcgctg tgccagtggg cgcaaagtct
    26161 gagcttgccc tccggtaaaa cgacctggtt taacttcctc gcctctcacg atggcatcgg
    26221 gctgaaccca ctgcggggct tgttaccaga aagcgaaata ttagcgctgg tcgaggcatt
    26281 acagcaggaa ggtgcattag taaactggaa aaataatccc gacggtacgc gcagtcccta
    26341 tgaaataaat gtcacctata tggatgcgtt aagccgccgc gagagtagcg atgaagaacg
    26401 ttgcgccagg tttatccttg cccatgcgat tttgttaagt ttccccggtg tgccagcgat
    26461 atatattcaa agtattctgg gctcgcgtaa tgattacgca ggtgtcgaaa aactcggata
    26521 taaccgtgcg attaaccgta aaaaatatca cagtgaagag ataacacgag aactgaacga
    26581 tgaagctaca ttaaggcatg cggtatatca tgagttgtcg cgtttaatta cacttcgtcg
    26641 cagccataac gagtttcatc cggataataa ttttaccatc gatacggtta attcatccgt
    26701 aatgcgtatt caaagaagta acgctgatgg taattgtctg actggattgt ttaatgtcag
    26761 taaaaatatt cagcatgtaa atattactga tctgcatggt cgggatctga ttagtgaagt
    26821 tgatatattg ggtaatgaaa taacgctgcg cccctggcag gttatgtgga ttaaataaaa
    26881 aggaacatct catgattaaa tcaaaaatcg tgctgttatc agcactggtt tcctgcgccc
    26941 tgatttcagg ctgtaaagaa gaaaataaaa cccatgtatc catcgaattt atgcattctt
    27001 cggtggagca ggagcgtcag gcagttatca gcaaattgat tgcccgtttt gaaaaagaaa
    27061 acccaggtat caccgttaaa caagtgcccg tggaagaaga cgcttataac accaaagtca
    27121 tcaccctttc acgtagcggc tcgctgccgg aagtgatcga aaccagccat gactacgcca
    27181 aagttatgga taaagagcag cttatcgatc gcaaagcggt tgccacggtc atcagcaacg
    27241 ttggtgaagg cgcgttttac gatggcgtac tgcgtattgt gcgtaccgaa gatggtcgcg
    27301 catggaccgg tgttcctgtc agcgcctgga ttggcggcat ctggtatcgc aaagatgtgc
    27361 tggcaaaagc ggggctggag gagccgaaaa actggcaaca gctgctggac gttgcacaga
    27421 aactgaatga cccggcgaat aaaaaatacg gtattgcgct gcctacggca gaaagcgtgc
    27481 tgacggaaca atccttctcc cagtttgcgt tatccaacca ggccaacgtc tttaacgccg
    27541 aaggcaaaat cacccttgat acaccagaga tgatgcaggc gctgacctat taccgcgacc
    27601 ttgctgccaa caccatgccg ggttctaacg acatcatgga agtgaaagac gcctttatga
    27661 acggcaccgc gccgatggcg atttactcca cctatatcct tccggctgta attaaagaag
    27721 gcgacccgaa aaacgtcggt ttcgtggtgc caaccgagaa aaactctgcc gtctacggca
    27781 tgttgacctc gctgaccatt accgccggac aaaagactga agaaacggaa gcggctgaaa
    27841 aatttgtcac ctttatggag caggcagaca acattgcaga ctgggtgatg atgtcgccag
    27901 gcgcggcact gccggtgaac aaagcggttg tcactaccgc cacctggaaa gacaacgacg
    27961 ttattaaggc gctgggtgaa ctaccgaatc agctaatcgg tgaactgcca aatattcagg
    28021 tctttggcgc agtaggggat aaaaacttta cccgcatggg tgatgtgacg ggttctggcg
    28081 tggtgagttc aatggtgcat aacgtcaccg tgggtaaagc cgatctctct actacgctgc
    28141 aagcgagcca gaaaaaactg gatgaactga tcgaacagca ctaaacccag gacaggaatc
    28201 cgcaatgaac aggctttttt caggtcgttc cgatatgctc tttgcgctgt tgcttctcgc
    28261 gcccagctta ttactgctgg gcggtctggt ggcgtggccg atggtgtcga atatcgaaat
    28321 cagtttttta cgtctgccgc tcaatcccaa catcgagcca acgtttgttg gggtaagcaa
    28381 ctatgtgcgt attctctccg atcccggctt ctggcactcg ctgtggatga cggtctggta
    28441 taccgcgctg gtggtggcgg gcagcaccgt gctcgggctg gccgtggcga tgtttttcaa
    28501 ccgcgagttc cgcctgcgta aaaccgcgcg ttcgctggtg atcctctcct acgtaacgcc
    28561 gtccatttcg ctggtgttcg cctggaaata catgttcaac aacggctacg gcattgtgaa
    28621 ctacctcggc gtcgatcttc tgcatcttta tgagcaggca ccgctgtggt tcgataatcc
    28681 gggcagtagt tttgtgctgg tggtgctgtt cgccatctgg cgctacttcc cgtatgcctt
    28741 tatctcgttt ctggcgattt tgcagaccat cgacaaatcg ctgtacgaag cggcagagat
    28801 ggatggcgct aatgcctggc aacggtttcg tatcgtcacg ctgcccgcga ttatgccggt
    28861 cctggcgacg gtggtgaccc ttcgcaccat ctggatgttc tacatgttcg cggatgttta
    28921 tctgctgacg accaaggtcg atattctcgg cgtttatctc tacaaaaccg cctttgcctt
    28981 taatgactta gggaaagcgg cggcgatctc ggtggtgctc ttcatcatta ttttcgctgt
    29041 cattctgctg accaggaaac gggtgaacct caatggcaac aaataaacgc acactcagtc
    29101 gaatcggttt ttactgcggg ctggcgctgt ttctcatcat cacgctgttt ccgttttttg
    29161 tgatgctgat gacttcgttc aagagcgcaa aagaggcgat ctcgctgcat cctacgctgc
    29221 tgtcgcaaca gtggacgctg gagcattatg tcgacatttt taacccggtg attttcccgt
    29281 ttgtcgacta cttccgtaac agcatggtgg tgtcggtggt ttcctccgtg gtggcggtat
    29341 ttctcggcat actcggggct tacgcgcttt ctcgcctgcg ctttaaaggc cggatgacca
    29401 tcaacgccag cttttacacg gtgtacatgt tctccggcat tttgctggtg gtgccgctgt
    29461 tcaaaatcat caccgcactt ggcatttacg acaccgagat ggcgctgatc attaccatgg
    29521 tgacgcaaac cctgcccacc gccgtgttta tgctgaaaag ctacttcgac accatccctg
    29581 atgagatcga agaagcggca atgatggatg gcctcaaccg tctgcaaatc atcttccgta
    29641 tcaccgtgcc cctggcgatg tccgggctga tatcggtgtt cgtctactgc tttatggtgg
    29701 cgtggaacga ctatctgttt gcgtcgattt tcctctccag cgccagcaat ttcaccttac
    29761 cagtgggcct gaacgcgctg ttcagtacgc ctgattacat ctggggacgg atgatggcgg
    29821 cctcactggt gaccgcattg ccggtggtga ttatgtatgc gctttcggaa cgttttatta
    29881 agagtggttt gaccgccggt ggcgtgaagg gctaaagcgg ccagtttctt tacaaggagt
    29941 tttacatgaa aaagttagta gccacagcac cgcgcgttgc tgcgctggtt gagtatgaag
    30001 atcgggcgat tttagccaat gaagtgaaga tccgcgtgcg tttcggcgca ccgaaacacg
    30061 gaacggaagt ggtcgacttc cgcgccgcca gcccgtttat cgatgaagac tttaacggcg
    30121 agtggcagat gttcaccccg cgtccggcag atgcgccgcg cggcattgag tttggcaaat
    30181 tccagcttgg caacatggtg gttggcgaca ttattgagtg cggcagcgac gttaccgacc
    30241 acgcggtggg cgacagcgta tgcggctacg gcccgctctc cgagacggtc atcattaacg
    30301 cagtgaataa ctacaagctg cgcaaaatgc cgcaaggcag ctcctggaaa aacgccgtct
    30361 gctacgaccc ggcgcagttc gccatgagtg gcgttcgcga tgccaacgta cgcgtagggg
    30421 attttgtagt ggtggtaggg cttggcgcga tcggtcaaat tgccatccaa ttggctaaac
    30481 gcgctggcgc gtcggtggta attggcgtcg atcctattgc ccatcgttgt gatattgctc
    30541 gtcgtcacgg tgcggatttc tgccttaatc ccattggcac tgacgtaggc aaagagatca
    30601 aaacgctgac cggcaagcag ggtgccgatg tgattatcga aaccagcggt tacgccgacg
    30661 cgctgcagtc ggcgctgcgc ggcctggctt acggtggcac catctcctat gtcgcgtttg
    30721 ctaaaccgtt tgccgaaggt tttaacctcg gacgcgaagc gcatttcaat aacgccaaaa
    30781 ttgtcttctc ccgtgcgtgc agcgaaccga acccggatta tccgcgctgg agccgtaagc
    30841 gtattgaaga aacctgctgg gaactgctga tgaacggtta tctaaattgc gaagatctaa
    30901 tcgacccggt ggtgaccttt gccaacagcc cggaaagcta catgcagtat gtcgatcagc
    30961 atccggaaca gagcatcaaa atgggcgtta cgttttaatt caaaggaatg aaaaaatgaa
    31021 aataggcaca cagaatcagg cattctttcc ggaaaatatc cgcgagaaat ttcgttatat
    31081 caaagagatg ggcttcgatg gttttgagat tgacggcaaa ctgctggtca acaacatcga
    31141 agaagtcaaa gcggcgatca aagaaaccgg tttaccggtg accaccgcct gcggtggcta
    31201 tgacggctgg attggtgact ttatcgaaga gcgtcgtctt aatggcttaa agcagataga
    31261 gcgcattctc gaagtgctgg cagaagtggg cggtaaaggt atcgtcgttc cggcagcgtg
    31321 gggcatgttt accttccgct taccgccgat gacctcgccg cgcagcctgg acggcgatcg
    31381 taaaatggtg agtgattccc tgcgcgtact ggaacaggtc gccgcgcgta ccggaaccgt
    31441 ggtgtatctc gaaccgttaa accgctatca ggatcatatg atcaacaccc tcgccgatgc
    31501 tcgtcgttac atcgtcgaaa acaatcttaa acatgtgcag attatcggcg atttctatca
    31561 catgaacatc gaagaagata acctggcgca ggcgctgcat gacaaccgcg acttgctcgg
    31621 tcatgtgcat attgccgata accatcgtta ccagccgggc agcggcaccc tcgatttcca
    31681 cgcgctgttt gaacagctgc gcgccgataa ctatcagggt tatgtggtgt atgaagggcg
    31741 tatccgggca gaagatcctg cccaggcgta ccgtgattcg ttggcttggt tgcgtacctg
    31801 ctaagaggtc tttgtgaaaa gtgcaatgac aagctctccg ctgcgtgtcg cgataatagg
    31861 cgcaggccag gtggcggata aggttcatgc ttcgtactac tgcacccgca acgttctgga
    31921 actggtggct gtctgtgaca gccgcctttc ccaggcgcag gcgctggcag aaaaatacgg
    31981 gaatgcatcc gtgtgggacg atccgcaggc catgctgctg gcggtgaaac ctgatgtggt
    32041 tagcgtctgc tcacctaacc gttttcatta cgaacatacc ctgatggcgc ttgaagcggg
    32101 ctgccatgtg atgtgcgaaa aaccgcccgc catgacgcca gaacaggcgc gggaaatgtg
    32161 cgatatcgcg cgcaaacagg gcaaggtgct ggcctacgac tttcaccatc gttttgcgct
    32221 cgatacgcaa cagctgcgtg aacaggtgac caacggcgtt ttgggagaga tttacgttac
    32281 caccgcccgc gccctgcgtc gctgcggcgt tcccggctgg ggtgtcttta ccaataaaga
    32341 actgcagggt ggtggcccgc tgatcgacat cggcattcat atgctggatg ctgcaatgta
    32401 tgtgctgggc tttccggcgg tgaaaagcgt gaatgcgcat agttttcaaa agatcggcac
    32461 gcaaaagagc tgtggtcaat ttggcgagtg ggatccggca acttatagcg tcgaagattc
    32521 gctgtttggc accattgaat ttcataacgg cggcattctg tggctggaaa cgtcatttgc
    32581 actcaacatc cgcgaacagt cgattatgaa cgtcagcttt tgcggtgata aagccggtgc
    32641 gacgctgttt ccagcacata tctacaccga taacaacggt gaattaatga cgctgatgca
    32701 acgggaaatg gcagacgaca accgccattt gcgcagcatg gaagccttta tcaatcacgt
    32761 acagggcaag cccgtgatga tagccgatgc cgagcagggg tacatcatcc agcaactggt
    32821 ggcggcgttg tatcaatccg cagaaacagg gacgcgtgtg gaattatgac caggccagta
    32881 acgttatcag aaccccattt cagccagcat accctgaaca agtatgcatc actgatggcg
    32941 caggggaacg gctatcttgg gcttcgcgcc agccatgaag aagattacac gcgccagacg
    33001 cgcggcatgt atctggcggg gctgtatcat cgggcgggta aaggtgaaat caacgaactg
    33061 gtgaacctgc ccgatgtggt ggggatggag attgccataa atggtgaggt tttctcatta
    33121 tcccgcgaag cctggcagcg tgagcttgac tttgccagtg gcgaattacg ccgcagcgtt
    33181 gtctggcgta ccagcaacgg cacaggttac accatcgcca gccgtcgctt tgtttcggca
    33241 gaccaactgc cgctcattgc gctggaaatc actattactc cactggacgc cgacgcgtca
    33301 gtgctgattt caacaggcat tgacgccacg caaaccaatc acggtcgcca acatttcgac
    33361 gaaacccagg tgcgggtgtt tggtcagcat ctgatgcagg ggatctacac cacccaggat
    33421 ggacgcagtg atgtggccat cagctgttgc tgtaaggtga gcggtgatgt gcagcaatgc
    33481 tataccgcca aagagcgccg tttgctgcaa cataccagcg cgcaacttca cgcaggcgaa
    33541 acgctgacgc tgcaaaaact ggtgtggatc gactggcggg atgacaggca agctgcttta
    33601 gacgagtggg gcagtgcgtc gcttcgccag cttgaaatgt gtgcgcagca gagttacgac
    33661 caacttcttg cagcatcaac agaaaactgg cgtcagtggt ggcagaaacg tcgtatcacg
    33721 gtaaacggcg gcgatgcgca cgatcagcaa gcgttagatt atgcgcttta tcatctgcgc
    33781 atcatgacgc ctgctcacga cgagcgcagc agtattgcgg caaaaggctt aaccggagaa
    33841 ggctacaaag gccacgtttt ctgggataca gaagtatttt tgctaccgtt tcatctgttt
    33901 agcgatccga cggttgcccg aagtttactg cgttatcgct ggcacaactt gccaggcgcg
    33961 caggagaaag cgcgacgcaa cggctggcag ggcgcgctat ttccgtggga gagcgcacgc
    34021 agtggtgaag aagagacgcc ggaatttgcc gccattaaca ttcgcaccgg gctgcggcaa
    34081 aaagtggcct cggcgcaggc tgaacatcat ctggtcgccg atatcgcctg ggcggttatt
    34141 caatactggc agacaacggg ggatgaaagt ttcattgctc atgaaggcat ggcgctactt
    34201 ctggagagcg caaagttctg gattagccgc gcggtaaggg ttaacgaccg tctggaaatt
    34261 catgatgtta ttgggccaga cgaatatacc gaacatgtca ataataacgc cttcaccagc
    34321 tatatggcgt attacaacgt ccagcaggcg ctgaacatcg ctcgtcaatt tggctgtagc
    34381 gacgttgcgt ttatccatcg cgccgaaatg ttcctcaaag agttgtggct gccagaaact
    34441 cagcccgacg gcgttttgcc gcaggatgat tcgtttatgg ctaagccggc gattaatctg
    34501 gcgaaataca aagcggcggc ggggaagcaa accattctgc tggattattc acgcgcagaa
    34561 gtgaacgaga tgcagatcct caaacaagct gatgtggtga tgctcaatta catgctgccg
    34621 gagcagttct cagcggcatc gtgtcttgcc aatctgcaat tttatgaacc gcgcactatt
    34681 cacgactcgt cattaagtaa agcaatccac ggcattgttg ccgcacgctg tggcctgctg
    34741 acccaaagtt atcagttctg gcgcgagggg actgaaatcg atcttggtgc tgatccgcat
    34801 agttgtgatg atggtatcca tgctgccgca actggcgcta tctggctggg ggcgattcag
    34861 ggttttgccg gggtgagcgt gcgtgacggt gaattgcatc taaatccggc gttacctgag
    34921 cagtggcaac agttgtcttt ccctctgttc tggcagggct gcgaattaca ggtcacgctc
    34981 gacgcgcagc gtattgcgat tcgaacttct gcgcccgttt cactgcgttt gaacgggcag
    35041 cttatatccg tggctgaaga atctgttttc tgtttggttg attttatttt gcccttcaat
    35101 gggaccgcta ccacgtatca ggaggatgaa tgaaactgca aggagtagtt ttcgacctgg
    35161 atggtgtaat caccgatacc gcgcatctgc atttccaggc gtggcagcag attgccgctg
    35221 aaattggcat cagcattgat gcgcagttta acgaatccct aaaagggatt agccgcgatg
    35281 agtctctgcg gcgcattctg caatacgggg gcaaagaggg cgactttaac tcgcaggagc
    35341 gggcgcaact ggcgtatcgc aaaaatctgc tctacgtcca ctcactgcgc gaactgacgg
    35401 ttaatgccgt tctgccgggg atccggcctt tgctggcaga tctccgcgca cagcagatcc
    35461 cggtagggct ggcttctgtc tccctgaatg cgccgacgat tttagcggcg ctggagctgc
    35521 gcgagttttt caccttctgc gcggatgctt cccaacttaa aaactcgaaa ccggatccgg
    35581 aaatctttct cgccgcctgt gccgggctgg gcgtgccgcc gcaggcatgt atcggcattg
    35641 aagatgcgca ggcgggcatt gacgccatta acgccagcgg tatgcgctcg gtggggatcg
    35701 gcgcaggctt aaccggggcg caattattgt tgcgctcaac ggactccctg acctggtcgc
    35761 ggttatcggc cttctggcaa aacgtatagc aaaggaatca acatggctca gctttcgtta
    35821 caacatattc aaaaaatcta cgataaccag gtgcatgtgg tgaaggactt caacctggaa
    35881 attgccgata aagagttcat cgtatttgtc ggcccgtcgg gctgcggtaa atcgaccacc
    35941 ctgcgcatga ttgccgggct tgaggagatc agcggcggcg agctgttgat cgacggcaaa
    36001 cgaatgaatg acgttccagc taaagcacgc aatatcgcga tggtgttcca gaactacgcg
    36061 ttgtatccgc atatgacggt ttacgacaac atggcgtttg gcctgaagat gcaaaaaatc
    36121 gccaaagagg tgattgatga gcgggtgaac tgggcggcgc aaattctcgg cctgcgtgag
    36181 tacctgaaac gcaagccggg cgcgctttcc ggcgggcaac gtcagcgtgt ggcgctcggg
    36241 cgggcgattg tacgcgaagc gggcgtgttt ttaatggatg aaccgctctc taaccttgat
    36301 gccaagctgc gcgtgcaaat gcgcgcagag atcagcaagc tgcatcagaa actgaacacc
    36361 accatgatct acgtgaccca cgatcagacc gaagcgatga ccatggcgac gcggattgtg
    36421 attatgaaag acgggattgt tcagcaagta ggtgcgccga aaaccgttta taaccaaccc
    36481 gcgaatatgt ttgttgccgg atttattgga tcaccagcga tgaattttat tcgcggcacg
    36541 atcgatggcg ataaattcgt tacggaaacg cttaaattaa ccattcccga agagaaatta
    36601 gcagttctga aaacacagga aagtttgcat aagcccatcg tgatgggaat acgcccggaa
    36661 gatattcatc cggacgcgca agaggaaaat aacatttccg ccaaaattag cgtggcagaa
    36721 ttaaccggtg cggaatttat gctctacacc acggttgggg ggcacgagtt agtggtccgt
    36781 gctggtgtgt taaatgatta tcatgcagga gaaaatatca ctattcattt tgatatgact
    36841 aaatgtcatt tctttgatgc agaaacggaa atagcaattc gctaaataca gggggaaggc
    36901 attcccccag gataatacaa ggaacaataa tgaaaaagtt attaccctgt accgcactgg
    36961 tgatgtgtgc gggaatggcc tgcgcacagg cagaggaaaa gaacgactgg cactttaata
    37021 tcggcgcgat gtacgaaata gaaaacgtcg agggttatgg cgaagatatg gatgggctgg
    37081 cggagccttc tgtctatttt aatgccgcca acgggccgtg gagaatttct ctggcctatt
    37141 atcaggaagg gccggtagat tatagcgcgg gtaaacgtgg aacgtggttt gatcgcccgg
    37201 agctggaggt acattatcag ttcctcgaaa gcgatgattt cagtttcggc ctgaccggcg
    37261 gtttccgtaa ttatggttat cactacgttg atgaatcggg taaagatacg gcgaatatgc
    37321 agcgctggaa aatcgcgcca gactgggatg tgaaactgac tgacgattta cgtttcaacg
    37381 gttggttgtc gatgtataaa tttgccaacg atctgaacac gaccggttac gctgataccc
    37441 gtgtcgaaac ggaaacaggt ctgcaatata ccttcaacga aacggttgcc ttgcgagtga
    37501 actattatct cgagcgcggc ttcaatatgg acgacagccg taataacggt gagttttcca
    37561 cgcaagaaat tcgcgcctat ttgccgctga cgctcggcaa ccactcggtg acgccgtata
    37621 cgcgcattgg gctggatcgc tggagtaact gggactggca ggatgatatt gaacgtgaag
    37681 gccatgattt taaccgtgtt ggtttatttt acggttatga tttccagaat ggtctttccg
    37741 tttcgctgga atacgcgttt gagtggcagg atcacgacga aggcgacagc gataaattcc
    37801 attatgcagg tgtcggcgta aattactcgt tctgataatg ggctaaattg ccggatgcgg
    37861 cgcgagtact ttatccgatc tataaatgta ggccggataa gatgcgctag catcgcatcc
    37921 ggcattcagg caaggtagct ggtatttatt tcagcgtcat atgcgtggca acggtaatat
    37981 tctgtggtga cggttttcca gaaattaagc ggaataataa ctcgcagctt tgttgaccta
    38041 actcctgcgt cggaacatcg atgccgcccg gtgcaggcgt taaaataaat gacagcgttt
    38101 cattgctata acccaccacc gctaactgct gcggaatagc aatgtttttc tctgccgccg
    38161 cacgataaat gctcattaat ttcaggctgt cagtggcaaa cactgcctca ggcaacggtg
    38221 actgacttaa taattgccgt gctgcttgca gtgctgtttc atgggtataa ccgccatcaa
    38281 caatccattc atcacgcact gcaatattat gatcttccag actttgctta taaccattta
    38341 cccgatccac tgaaacatgg acatcaagcg gtgcatgcag acaggcaata ttttgatgtc
    38401 cactttcaat taacgcatcg gtcaacgcaa tgctgtcgcc aaaattatcg gtatcgacag
    38461 aataaacatg ggcatattga ccttcaactt tgccaatcac caccaccgga atatcgtatt
    38521 tatcaagttg ggcaaaaaat gactcatccg ctggcgaact tagcataata atgcctttaa
    38581 tcattttctg cttaattttg ctttcacatt tttgtaagtc ttctgccagg ttgtgcgacg
    38641 tctgcaatat cacgtcgaaa ccttcttctt cagctttggc ggtgatggca tgcaaaactt
    38701 cagagaaaaa cggattaccc gccgtagttt tggtcgaacg ggtagaaatc accataatgg
    38761 catcaaaacc cgaagaggtc agcgcgcggg ccagcttgtt tggttgatac tgtaattctt
    38821 caatggcccg taacactttt tcgcgcgctt ccggggagat attagtttgc ttattcagca
    38881 cgcgtgatac ggtggatttt gatacgcctg caaccctggc aatatcataa atagtagggg
    38941 acataggtca ggaactccgt ccgaacgtga atgctgcaca tcttatggag ttcgcccgtc
    39001 gatgacaaca cgggcagttg ataatcaatg gcctggcccc cacattcata tccttatgaa
    39061 tgattttttt tctggtcttc agagcagcac aggacagcaa tgaagcgact taaaaatgaa
    39121 cttaatgcgc tggtgaatcg gggtgtcgac agacatctgc gcctcgccgt aaccggactt
    39181 agccgcagcg gcaaaacggc gtttatcact gcgatggtca atcagttgct caatattcac
    39241 gccggagcac gcttgccgct gttaagcgcg gtgcgtgaag aacggctgct gggcgtaaaa
    39301 cgcattcccc agcgtgactt tggcattccg cgcttcacct atgatgaagg gctggcgcag
    39361 ttatatggcg atccacctgc ctggccaacg ccaacgcgcg gcgtcagcga aattcgcctg
    39421 gcgctacgtt ttaaatcgaa cgattcgctg ctacgccact ttaaggatac ctccacgctg
    39481 tatctggaga ttgtggatta tcccggcgaa tggttgctcg acctgccgat gctggcgcag
    39541 gactatttaa gctggtcgcg ccagatgacg ggcttactta atggtcagcg cggcgaatgg
    39601 tcggcgaaat ggcgaatgat gtgcgaaggg ctggacccgc tagcgcctgc cgacgaaaac
    39661 cggctggcag acattgccgc cgcgtggacc gattatctcc accactgtaa acagcagggg
    39721 ctgcacttta ttcagcctgg gcgctttgtc ttgccggggg atatggcagg tgcgcccgcg
    39781 ctgcaattct tcccgtggcc ggatgtcgat gcctggggcg agtccaaact ggcgcaggcc
    39841 gataagcaca ccaatgccgg aatgctgcgc gagcggttta attattactg cgagaaggtg
    39901 gtgaaggggt tctataagaa tcattttctg cgctttgacc gccagattgt gctggtggat
    39961 tgcctgcagc ctctcaacag tgggccacag gcatttaatg atatgcgtct ggcactgacg
    40021 cagctgatgc aaagtttcca ctacgggcag cgcacgctgt ttcgccgctt attctcgccg
    40081 gttatcgaca aactgctgtt tgccgcgacg aaagcggacc atgtaacggt cgatcagcac
    40141 gccaatatgg tttcattact gcaacaactg attcaggatg cctggcaaaa tgcggcgttt
    40201 gaagggatta gtatggattg cctggggctg gcctcggtgc aggcgaccac cagcggcatt
    40261 attgacgtca acggcgagaa aattccggca ttgcgcggta accggctcag cgatggtgcg
    40321 cctctcaccg tttaccccgg tgaagttcct gcgcgtttgc ccggtcaggc gttctgggat
    40381 aagcaaggct tccagtttga ggcatttcgt ccgcaggtga tggatgtcga caaaccacta
    40441 ccgcatattc gtcttgatgc tgcgctggaa tttttaatag gagataaatt gcgatgaccg
    40501 aaccgttaaa accacgtatt gatttcgacg gtccgctgga ggtcgatcag aatccaaaat
    40561 tcagggcgca gcagaccttt gacgaaaatc aggcgaaaaa ttttgccccg gccacgctcg
    40621 acgaagcgca ggaagaagag gggcaagtcg aagcggtaat ggacgcagcg ttacgtccga
    40681 aacgcagcct gtggcgcaaa atggtgatgg gcgggctggc tctgtttggc gcaagcgttg
    40741 tcgggcaggg tatacagtgg acaatgaatg cctggcaaac ccaggactgg gtagcgctgg
    40801 gtggatgtgc cgctggggca ttgattatcg gcgctggcgt aggttccgtg gtaacagagt
    40861 ggcggcgctt atggcgcttg cgacagcgcg cccatgaacg cgacgaagcg cgtgatttat
    40921 tgcatagcca cggaaccggc aaaggccgcg cattttgcga aaaactggcg cagcaggcgg
    40981 gcatcgatca gtcacatccg gcgctgcaac gctggtatgc ctcaatccat gaaacgcaaa
    41041 acgaccgtga agtggtcagt ttgtatgcgc atttggtcca gccagtttta gatgcccagg
    41101 cgcggcgcga aatcagccgt tcggcagcgg aatcaacgtt gatgattgcg gtcagcccgc
    41161 tggcgctggt ggatatggca tttatcgcct ggcgcaatct gcgtttgatt aatcgcatcg
    41221 ccacgctgta tggcattgaa ctggggtatt acagccgttt gcgcctgttc aagctggtat
    41281 tgctgaatat cgctttcgcc ggagccagcg aattggtgcg cgaagtgggg atggactgga
    41341 tgtcgcaaga tctcgctgct cgtttgtcta cccgcgcagc tcaggggatt ggtgctgggc
    41401 ttctgacggc acgactcggg attaaagcta tggagctttg ccgcccactg ccgtggattg
    41461 acgatgacaa acctcgcctt ggggatttcc gtcgtcagct tatcggtcag gtgaaagaaa
    41521 cgctgcaaaa aggcaaaacg cccagcgaaa aataatgcaa tatcgggtgc tgaccggata
    41581 tctttacgcc gaagtgcccg tttttccgtc tttgtgtcaa tgattgttga cagaaacctt
    41641 cctgctatcc aaatagtgtc atatcatcat attaattgtt cttttttcag gtgaaggttc
    41701 ccatgcgtct ggaagtcttt tgtgaagacc gactcggtct gacccgcgaa ttactcgatc
    41761 tactcgtgct aagaggcatt gatttacgcg gtattgagat tgatcccatt gggcgaatct
    41821 acctcaattt tgctgaactg gagtttgaga gtttcagcag tctgatggcc gaaatacgcc
    41881 gtattgcagg tgttaccgat gtgcgtactg tcccgtggat gccttccgaa cgtgagcatc
    41941 tggcgttgag cgcgttactg gaggcgttgc ccgaacctgt gctctctgtc gatatgaaaa
    42001 gcaaagtgga tatggcgaac ccggcgagct gtcagctttt tgggcaaaaa ttggatcgcc
    42061 tgcgcaacca taccgccgca caattgatta acggctttaa ttttttacgt tggctggaaa
    42121 gcgaaccgca agattcccat aacgagcatg ttgttattaa tggacagaac ttcctgatgg
    42181 agattacacc tgtttatctt caggatgaaa atgatcaaca cgtcctgaca ggtgcggtgg
    42241 tgatgttgcg atcaacgatt cgtatgggcc gtcagttgca aaatgtcgcc gcccaggacg
    42301 tcagcgcctt cagtcaaatt gtcgctgtca gcccgaaaat gaagcatgtt gtcgaacagg
    42361 cgcagaaact ggcgatgcta agcgcgccgc tgctgattac gggtgacaca ggtacaggta
    42421 aagatctttt tgcctacgcc tgccatcagg caagcccaag agcgagcaaa ccttacctgg
    42481 cgctgaactg tgcgtctata ccggaagatg cggtcgagag tgagctgttt ggtcatgctc
    42541 cggaagggaa gaaaggattc tttgagcagg cgaacggtgg ttcggtgctg ttggatgaaa
    42601 taggtgagat gtcaccacgg atgcaggcga aattactgcg tttccttaat gatggcactt
    42661 tccgtcgggt tggcgaagac catgaggtgc atgtcgatgt gcgggtgatt tgcgctacgc
    42721 agaagaatct ggtcgaactg gtgcaaaaag gcatgttccg tgaagatctc tattatcgtc
    42781 tgaacgtgtt gacgctcaac ctgccgccgc tacgtgactg tccgcaggac atcatgccgt
    42841 taactgagct gttcgtcgcc cgctttgccg acgagcaggg cgtgccgcgt ccgaaactgg
    42901 ccgctgacct gaatactgta cttacacgtt atgcgtggcc gggaaatgtg cggcagttaa
    42961 agaacgctat ctatcgcgca ctgacacaac tggacggtta tgagctgcgt ccacaggata
    43021 ttttgttgcc ggattatgac gccgcaacgg tagctgtggg cgaagatgcg atggaaggtt
    43081 cgctggacga aattaccagc cgttttgaac gctcggtatt aactcagctt tatcgcaatt
    43141 atcccagcac gcgcaaactg gcaaaacgtc tcggcgtttc gcataccgcg attgccaata
    43201 agttgcggga atatggtctg agtcagaaga aaaacgaaga gtaagcgcga atatgcctga
    43261 cggtgcaaca ccatcaggca tattaactta tgctttcagt acagccagag ctgcttcgta
    43321 atccggctcg gtggtgattt catccaccag ctggctgaaa atcacattgt cattttcatc
    43381 aataaccaca acggcacgcg ctgccagacc tttcagtggg ccatcagcaa ttgccacacc
    43441 gtaggcttgc aggaattcag cgttacggaa agtggagagg gtgataacgt tgttcagacc
    43501 ttctgcgccg cagaaacgag actgggcgaa cggcagatcg gcagaaatac acagcacaac
    43561 ggtgttgtcg atctcggttg ccagttggtt aaacttacgt actgatgcgg cgcaaacacc
    43621 ggtatcaata ctcgggaaaa tgttcagcac tttgcgttta cccgcaaact gaccgagggt
    43681 gacgtcagac agatcttttg ccacgagagt aaaagtctgc gctttgctac ccgcctgtgg
    43741 gatggaattg gcgactgtaa ccgggttgcc ctggaaatga acggtttgtg acatgattat
    43801 ctttcctgtt tacatatagt taacgtcaca cctagtttat gctaactgtc aataacacag
    43861 caaacgctat ttgcgcttaa tccgcagacc accgcgacaa caaggagtaa agatgagaac
    43921 cgttaaggta ttcgaggaag cctggccctt acataccccg tttgtgattg cccggggaag
    43981 tcgcagtgaa gcgcgcgtgg tggtggttga actggaagaa gagggtatta aaggcaccgg
    44041 cgaatgcacg ccgtatccgc gttatggaga aagtgatgcc tcggtaatgg cgcaaattat
    44101 gagcgtcgtg ccgcaattag agaaagggct gacacgggag gagttgcaaa aaattctccc
    44161 tgccggcgca gcacgtaatg cgctggattg tgcattgtgg gatctggccg cgcgaaaaca
    44221 gcagcaatcg ctggctgatt tgatcggcat aacgcttccc gggacagtta tcactgcaca
    44281 gacggttgtc atcggtacgc ctgatcagat ggccaatagt gcatcaacac tctggcaggc
    44341 cggcgcgaaa ttactgaaag tgaagctgga taaccatctt atcagtgagc ggatggtggc
    44401 aattcgcaca gctgtgcccg atgccacgct gatcgttgat gcaaatgaat cctggcgtgc
    44461 agaagggttg gcggcacgtt gccagctatt ggcggattta ggcgttgcga tgcttgaaca
    44521 accgcttcct gcgcaggacg atgcggcgct ggagaatttt attcatccgt tgccgatttg
    44581 tgctgatgaa agttgtcata ctcgtagcaa tttgaaggcg ctgaaagggc gctatgagat
    44641 ggttaatatt aagctcgata aaaccggtgg tctgacggaa gcgctggcgc tggcaactga
    44701 agcgcgtgca caaggttttc gtctgatgct gggctgcatg ttgtgtacct ctcgtgccat
    44761 tagcgccgct ttaccgctgg tgccgcaggt cagtttcgcc gatcttgacg gaccgacctg
    44821 gctggcggta gatgtggaac cagcgcttca gttcacgacg ggcgaattgc atctttagga
    44881 tgccagcgca gcaagtttgc catcgcaaag agatattttt cgctggcttc gtcggaggag
    44941 atgggcggaa actctgcggt gatgcaatgc aagttcagat ctgcacacca actgccgaaa
    45001 gagccaggcg tttcataacc gacgctggtt accagaggca attcaaacgc ctgagccagc
    45061 cattcgccta attcgctgtg tctgggatct tcaatacagg ccagtggatc gtggaatgag
    45121 accacccagg caggttgaat gcggtgtata agctggcaca gcgcctgggt ttcaggttcg
    45181 gagccaggtt tgtcacctgt cagtaatacg acatcacgtt cttcagcggc gctgttccag
    45241 cgataaaccg tttcaccttc cttccagttc gccgccggaa agtttctgtt taaatccaca
    45301 ccattagcat tggcccgtaa ccccagctgg cagccgtcag gattcacaca cagcaccaca
    45361 tgatggcggc gcaaagaagg tgtcaaagtc cgcagagcac aggagagcgt gacgaccgaa
    45421 gagttttcat caccgtgagt gccagccaga atcaaaccac tttcgcgact ggctgcaggg
    45481 gccggaaacc agattaacgg cgcgcccaat aatgaacgtc cgtaatgttc tgttccgggc
    45541 ggaaatgcgc cgcgttcggc gcgtgggcgg gttacggtca tgatcgtctc tggttcagga
    45601 agtattacag gcagtgttgt gcaaattttg cggattatca aatccttttc ttcaacgcta
    45661 tgttatcgat cttgtatcac ttttttgatg gaagttgttt gcatttttat tgatgtgcag
    45721 atattagaat tatgctgact tagatgtaag tcgtaatgtt atatacatgg cgtattttat
    45781 tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt aataagttta
    45841 ataaatcgta ctgcacatag tacagtacga tggtagctat ataattgatt tacatgaaga
    45901 atggcttatc gccatcagtc ccgggttgat tgatgcctat attctcacca atgtgtttct
    45961 caggtttatc gatgatatct gtaattaatg ctgcgacact cttacgtgat acgatcgtac
    46021 ctttgaatgg ttccttgcgt gaggtaagct cataatcaat aatatcttca tccgtcagcc
    46081 aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc gcagccctgc
    46141 ggaatggttt taggggctca ccaataactg cattattcca ttcgccaaat tttcctggaa
    46201 cttcatcata aatgccgagg gaaagaacaa agatcagacg tttaacatca caggctttca
    46261 tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg ttggcataca
    46321 caatatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca tcccccataa
    46381 taatctggct attagttgga tagggtttgt gtatttttgc tggctgacga gcaaataaag
    46441 tttgtttaat ggtttgttta tctgccagtt gattaatcac atggcgggct atctgaccgc
    46501 cagcaccaag aatgagtaca tttttcataa tatttctcat gaaacttatc acagatgggt
    46561 gttgaaaaaa ggaataactt tttccagcgc ctcctgaact gcttccggtt tgtcatacag
    46621 ttcataatgt gagaaatcgg gtaatgaaac taactgacga tcctgcgaaa ccatcgcccg
    46681 gccatagaat tccagtccat cacgataagc accgaaggca ccgacttttt caccaactac
    46741 taccatcacc ggctgagtca gtaacacttc agtaaatgcg aaggcatccc aggccagcgt
    46801 tttctgtgca tgtgagaaca acatttttgt cgcacctccc ggttgttgcc cgcgtggtgt
    46861 tttatagtag tcagttgctt cataaacatc gcgttcggtc agtccatggg cttttgctgc
    46921 atccaggctg gcgggaagta attcgttgat ctgtaattct ccaccacgag cctctttggt
    46981 tcgttgcgac gccattgcat tcaatgcgcc aatcggatcg taattgctga aaccttcccg
    47041 aaaaagacgc ccgatattaa cacccgtaat actcactaca gctttaattc gtttttctgt
    47101 cagcgcggca ttaatggcat agccaccccc cccacacaca cccagcacgc caatgcgttc
    47161 agcatcaaca tatggcagag ttactgcata atcgataaca cggctaatat cctctacgcg
    47221 ctgtgtcgga tcttctatcc aacgaggctc cccaccagat tctccctaga aactggcgtc
    47281 ataagcgaga acgacatagc ctttttcagc caatgccttg ccatagatat tgccagacgt
    47341 ctgctcctta caactgccaa aaggatggac gctgataatc gccggatact gttttgactc
    47401 atcaaaattt ggtggaaact gaatattggc agcaatatgc caggccatat cactattgcg
    47461 gatttgaata tgtttcatta cagagtttcc tcaaagaacg gagctaatac tgaaattgct
    47521 tcagccacat aggctttgcc gtcataaaga tccatatggt ttgcgccttc aacaatgtga
    47581 tagcgtttat cctggcttga tgctcgatcg tacaggtcgt cactcatcca tttgctccct
    47641 gcctggctgc ccgccacaat ctgcgtcggc tgagtcaggt acacttccgc catatggtaa
    47701 gcatcatagg taataatctg gttaaggctg cgcaaagtag cgtaacccgg tgctgttgga
    47761 tactgcgcgc gaggggtgtg gtaatactcc caggcctgac gcagttcttc gttcggcgca
    47821 tcggactcct tcattggtgc cagtggcata atggcatatt ctccgctgct aatatcgctg
    47881 gttcgggcgt ttgaacccgc gtcaacgtat ggcagggcat caatagattt cacattgttt
    47941 tcccaaccat tacggaacat cgaaccaata ttgaccgcac taacggtacc gatggccttg
    48001 atgcggcgat cctgaattgc agcattggct gtatatcctg caccggcaca aattcccatc
    48061 gcaccaattc gggtattgtc gacataagaa agcgttgtca ggtaatcaat cacggcactg
    48121 atgtcttcag tacgaatgta tgggttttct aactgacgcg gctcgccgcc actttcacct
    48181 tgataagatg cgtcataagc aatagtgaca aatccctttt ccgccagttt ttcggcataa
    48241 gctccggccg tttgttcttt aacgccccca cctgggtgag ataccacaat tgccggatgc
    48301 tgacgggttt catcaaattt tggagggaaa tagatcactg cagacaaaga gatggttgga
    48361 ttattgctgt tagtgaagct gactttatta ttcatcattc cgttcctcat ggagttgttg
    48421 gttcgtttta acggttggtg atatcactat agatattgat cattaagttg attagaccca
    48481 aaatcatgat tagacctatc atttaaatga ttaatagatt gtatggagat actgaatgaa
    48541 gcgtgaagag atcgctgatc tgatggcgtt tgtcgtcgtt gcagaggagc gtagcttcac
    48601 tcgagcagca gcccgcctga gcatggcgca gtcagcttta agccagatag tgcgccgtat
    48661 agaagaacga ttgggattgc ggcttctgac gcgaactacg cgcagcgttg taccaactga
    48721 agcgggcgag catcttttgt ctgttcttgg ccctatgttg catgacatag attctgccct
    48781 gacatccctg agcgatctgc agaaccgccc atccgggaca atacgtatta ctactgtaga
    48841 acatgcagca aaaacgatat tgttaccagc catgcgcaca ttcctgagat cgcatcctga
    48901 aattgatatt cagctcacca ttgattatgg tttgaccgat gtcgtttctg aacgttttga
    48961 tgcaggcgtc cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc
    49021 agatatacca atggctattg ttggctcacc ggattatttt tctcgccgaa gtgttccaac
    49081 gtcagtgtca caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac
    49141 agcaaatcgc tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca
    49201 gcttttactg aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc
    49261 gtatctacct tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt
    49321 ggataaattc acaccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc
    49381 tggctcggca ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta
    49441 cttgctgata cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt
    49501 ggttattttt tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt
    49561 ttgaacgtgc gaagcccgaa cgtagtgttc ggtgttccat gtgctttgcc gcattttggc
    49621 g
//
LOCUS       DAZUGK010000010        47749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZUGK010000010 DAZUGK010000000
VERSION     DAZUGK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.010"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..696
                     /gene="comR"
                     /locus_tag="ACHY5W_000498"
     CDS             64..696
                     /gene="comR"
                     /locus_tag="ACHY5W_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFS4445460.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(758..1297)
                     /locus_tag="ACHY5W_000499"
     CDS             complement(758..1297)
                     /locus_tag="ACHY5W_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS4445461.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1524..2828)
                     /gene="ndh"
                     /locus_tag="ACHY5W_000500"
     CDS             complement(1524..2828)
                     /gene="ndh"
                     /locus_tag="ACHY5W_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFS4445462.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3228..3770)
                     /gene="ycfP"
                     /locus_tag="ACHY5W_000501"
     CDS             complement(3228..3770)
                     /gene="ycfP"
                     /locus_tag="ACHY5W_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFS4445463.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3793..4818)
                     /gene="nagZ"
                     /locus_tag="ACHY5W_000502"
     CDS             complement(3793..4818)
                     /gene="nagZ"
                     /locus_tag="ACHY5W_000502"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFS4445464.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAEHVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4829..5653)
                     /gene="thiK"
                     /locus_tag="ACHY5W_000503"
     CDS             complement(4829..5653)
                     /gene="thiK"
                     /locus_tag="ACHY5W_000503"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFS4445465.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKCLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5634..6275)
                     /gene="lpoB"
                     /locus_tag="ACHY5W_000504"
     CDS             complement(5634..6275)
                     /gene="lpoB"
                     /locus_tag="ACHY5W_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFS4445466.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6289..6666)
                     /locus_tag="ACHY5W_000505"
     CDS             complement(6289..6666)
                     /locus_tag="ACHY5W_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFS4445467.1"
                     /translation="MRKGCFGLMSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6669..7028)
                     /gene="hinT"
                     /locus_tag="ACHY5W_000506"
     CDS             complement(6669..7028)
                     /gene="hinT"
                     /locus_tag="ACHY5W_000506"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFS4445468.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7362..9551
                     /gene="fhuE"
                     /locus_tag="ACHY5W_000507"
     CDS             7362..9551
                     /gene="fhuE"
                     /locus_tag="ACHY5W_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFS4445469.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9611..11044)
                     /gene="ptsG"
                     /locus_tag="ACHY5W_000508"
     CDS             complement(9611..11044)
                     /gene="ptsG"
                     /locus_tag="ACHY5W_000508"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFS4445470.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11339..12136)
                     /locus_tag="ACHY5W_000509"
     CDS             complement(11339..12136)
                     /locus_tag="ACHY5W_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4445471.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRHLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12147..13151)
                     /gene="holB"
                     /locus_tag="ACHY5W_000510"
     CDS             complement(12147..13151)
                     /gene="holB"
                     /locus_tag="ACHY5W_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4445472.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPLRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13148..13789)
                     /gene="tmk"
                     /locus_tag="ACHY5W_000511"
     CDS             complement(13148..13789)
                     /gene="tmk"
                     /locus_tag="ACHY5W_000511"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4445473.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13779..14801)
                     /gene="yceG"
                     /locus_tag="ACHY5W_000512"
     CDS             complement(13779..14801)
                     /gene="yceG"
                     /locus_tag="ACHY5W_000512"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFS4445474.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGIWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14804..15613)
                     /gene="pabC"
                     /locus_tag="ACHY5W_000513"
     CDS             complement(14804..15613)
                     /gene="pabC"
                     /locus_tag="ACHY5W_000513"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFS4445475.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15733..16974)
                     /gene="fabF"
                     /locus_tag="ACHY5W_000514"
     CDS             complement(15733..16974)
                     /gene="fabF"
                     /locus_tag="ACHY5W_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4445476.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17062..17298)
                     /gene="acpP"
                     /locus_tag="ACHY5W_000515"
     CDS             complement(17062..17298)
                     /gene="acpP"
                     /locus_tag="ACHY5W_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4445477.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17509..18243)
                     /gene="fabG"
                     /locus_tag="ACHY5W_000516"
     CDS             complement(17509..18243)
                     /gene="fabG"
                     /locus_tag="ACHY5W_000516"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFS4445478.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18256..19185)
                     /gene="fabD"
                     /locus_tag="ACHY5W_000517"
     CDS             complement(18256..19185)
                     /gene="fabD"
                     /locus_tag="ACHY5W_000517"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4445479.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19201..20154)
                     /gene="fabH"
                     /locus_tag="ACHY5W_000518"
     CDS             complement(19201..20154)
                     /gene="fabH"
                     /locus_tag="ACHY5W_000518"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4445480.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20222..21292)
                     /gene="plsX"
                     /locus_tag="ACHY5W_000519"
     CDS             complement(20222..21292)
                     /gene="plsX"
                     /locus_tag="ACHY5W_000519"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4445481.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGCSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21373..21546)
                     /gene="rpmF"
                     /locus_tag="ACHY5W_000520"
     CDS             complement(21373..21546)
                     /gene="rpmF"
                     /locus_tag="ACHY5W_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4445482.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21598..22119)
                     /gene="yceD"
                     /locus_tag="ACHY5W_000521"
     CDS             complement(21598..22119)
                     /gene="yceD"
                     /locus_tag="ACHY5W_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFS4445483.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22318..22902
                     /gene="yceF"
                     /locus_tag="ACHY5W_000522"
     CDS             22318..22902
                     /gene="yceF"
                     /locus_tag="ACHY5W_000522"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFS4445484.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENTRLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23014..23973)
                     /gene="rluC"
                     /locus_tag="ACHY5W_000523"
     CDS             complement(23014..23973)
                     /gene="rluC"
                     /locus_tag="ACHY5W_000523"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFS4445485.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24546..27731
                     /gene="rne"
                     /locus_tag="ACHY5W_000524"
     CDS             24546..27731
                     /gene="rne"
                     /locus_tag="ACHY5W_000524"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFS4445486.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQAAPKVEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVHV
                     EEQREQEEAQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEPPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAKTAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27927..28880)
                     /gene="flgL"
                     /locus_tag="ACHY5W_000525"
     CDS             complement(27927..28880)
                     /gene="flgL"
                     /locus_tag="ACHY5W_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS4445487.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28892..30535)
                     /gene="flgK"
                     /locus_tag="ACHY5W_000526"
     CDS             complement(28892..30535)
                     /gene="flgK"
                     /locus_tag="ACHY5W_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFS4445488.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQVLIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNLVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30601..31542)
                     /gene="flgJ"
                     /locus_tag="ACHY5W_000527"
     CDS             complement(30601..31542)
                     /gene="flgJ"
                     /locus_tag="ACHY5W_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFS4445489.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31542..32639)
                     /gene="flgI"
                     /locus_tag="ACHY5W_000528"
     CDS             complement(31542..32639)
                     /gene="flgI"
                     /locus_tag="ACHY5W_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS4445490.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32651..33349)
                     /gene="flgH"
                     /locus_tag="ACHY5W_000529"
     CDS             complement(32651..33349)
                     /gene="flgH"
                     /locus_tag="ACHY5W_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS4445491.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33402..34184)
                     /gene="flgG"
                     /locus_tag="ACHY5W_000530"
     CDS             complement(33402..34184)
                     /gene="flgG"
                     /locus_tag="ACHY5W_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS4445492.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34356..35111)
                     /gene="flgF"
                     /locus_tag="ACHY5W_000531"
     CDS             complement(34356..35111)
                     /gene="flgF"
                     /locus_tag="ACHY5W_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFS4445493.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNTVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35131..36336)
                     /gene="flgE"
                     /locus_tag="ACHY5W_000532"
     CDS             complement(35131..36336)
                     /gene="flgE"
                     /locus_tag="ACHY5W_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS4445494.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGAVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTADHAMTLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGGNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36361..37056)
                     /gene="flgD"
                     /locus_tag="ACHY5W_000533"
     CDS             complement(36361..37056)
                     /gene="flgD"
                     /locus_tag="ACHY5W_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFS4445495.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37068..37472)
                     /gene="flgC"
                     /locus_tag="ACHY5W_000534"
     CDS             complement(37068..37472)
                     /gene="flgC"
                     /locus_tag="ACHY5W_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS4445496.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37476..37892)
                     /gene="flgB"
                     /locus_tag="ACHY5W_000535"
     CDS             complement(37476..37892)
                     /gene="flgB"
                     /locus_tag="ACHY5W_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFS4445497.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38047..38706
                     /gene="flgA"
                     /locus_tag="ACHY5W_000536"
     CDS             38047..38706
                     /gene="flgA"
                     /locus_tag="ACHY5W_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS4445498.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38782..39075
                     /gene="flgM"
                     /locus_tag="ACHY5W_000537"
     CDS             38782..39075
                     /gene="flgM"
                     /locus_tag="ACHY5W_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS4445499.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39080..39496
                     /gene="flgN"
                     /locus_tag="ACHY5W_000538"
     CDS             39080..39496
                     /gene="flgN"
                     /locus_tag="ACHY5W_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFS4445500.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39536..41071)
                     /gene="murJ"
                     /locus_tag="ACHY5W_000539"
     CDS             complement(39536..41071)
                     /gene="murJ"
                     /locus_tag="ACHY5W_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFS4445501.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41181..42104)
                     /locus_tag="ACHY5W_000540"
     CDS             complement(41181..42104)
                     /locus_tag="ACHY5W_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4445502.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42106..42753)
                     /locus_tag="ACHY5W_000541"
     CDS             complement(42106..42753)
                     /locus_tag="ACHY5W_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFS4445503.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42764..43348)
                     /gene="rimJ"
                     /locus_tag="ACHY5W_000542"
     CDS             complement(42764..43348)
                     /gene="rimJ"
                     /locus_tag="ACHY5W_000542"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4445504.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43584..44792
                     /gene="mdtH"
                     /locus_tag="ACHY5W_000543"
     CDS             43584..44792
                     /gene="mdtH"
                     /locus_tag="ACHY5W_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFS4445505.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44856..45503
                     /gene="grxB"
                     /locus_tag="ACHY5W_000544"
     CDS             44856..45503
                     /gene="grxB"
                     /locus_tag="ACHY5W_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFS4445506.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSHVADYRDNMAKQTQINLLSSMAI"
     gene            45637..46197
                     /locus_tag="ACHY5W_000545"
     CDS             45637..46197
                     /locus_tag="ACHY5W_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4445507.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46303..47349
                     /gene="pyrC"
                     /locus_tag="ACHY5W_000546"
     CDS             46303..47349
                     /gene="pyrC"
                     /locus_tag="ACHY5W_000546"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4445508.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTIEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47423..47668
                     /gene="dinI"
                     /locus_tag="ACHY5W_000547"
     CDS             47423..47668
                     /gene="dinI"
                     /locus_tag="ACHY5W_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFS4445509.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11535 a        12910 c        11863 g        11441 t
ORIGIN      
        1 gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag gtataacggc
       61 atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc
      121 gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg
      181 acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa
      241 tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc
      301 gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac
      361 tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg
      421 atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca
      481 cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg
      541 gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg cattattcag
      601 gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga
      661 acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct
      721 gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca
      781 gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc
      841 cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat
      901 acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct
      961 ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac
     1021 cacgaccacc accaaactga tgccctatca cgccgccagc aacagctccg agcaccgacc
     1081 cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact
     1141 cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt
     1201 gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgccaagc gcagctgcga
     1261 caccaatccc gatacccgcc aacattgatt tattcacggg aatacctcct tctttgcagt
     1321 tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg
     1381 gaatggctgg aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt
     1441 aacgcatctt atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg
     1501 ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta
     1561 atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc
     1621 attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg
     1681 cgcgtcaggt tacccatcag gctaccgacg gtggagaagt tcgacagcga taccagcgaa
     1741 ccatgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc cagaatgttg
     1801 ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct
     1861 tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt
     1921 tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc accgatatct
     1981 ttcaggaagt ctggcgcttt gatcccggct gcccatacca tcagatcagc ctcaatatat
     2041 tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc
     2101 acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat
     2161 gcaggcaaaa tacgttcacc cgcttctacc agcgttacgt tcagggcttc gttggtcagg
     2221 cctttgtaac cgtagctgtg cagttgcttg accgcgttgt gcagttcagc ggagagttct
     2281 acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg
     2341 gcggagtatt tcaggaagag attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg
     2401 ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta
     2461 cccagcgcca ttaccagtgt gtcataggcg attttacgtt ccggaaccag cagttcacct
     2521 ttctcatcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg
     2581 gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg
     2641 acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca caggtggctg
     2701 tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt atgtcccagc
     2761 tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc
     2821 gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata
     2881 gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg
     2941 aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg
     3001 cgactttacc gggtgagatc aaaaaacaga gtttattgtt tattatttga gcgaatttgt
     3061 gagagtgata tagagtgagg ggtgtatgta gggcgttcgg taggcctgat aagaggcgca
     3121 tcaggcattg atgctcgaca gtcggatgcg gcgtaaacgc cttatccgac ccacagaatc
     3181 cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt
     3241 ttgaacgctt taatgcgctg taaatgcggg gagatattct tgaatttgtg cgtctgctct
     3301 tcgtcccaga caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagc
     3361 gcttcatcat tacgcgacaa aatcaccagg caacgatcgc gattcttctc acggaagttg
     3421 gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg
     3481 ttctcataag ggaacaaatt agggttgaag atcacctggc ggatgtcgca aagaaaacca
     3541 atccgttccg cccagtatcc gcccaggcca acgccgcaaa ttagcggacg ctcgtcaacg
     3601 ttcagttgca acattttgtc cacttcttta agcagatgct gcatatcatg tttcggatgc
     3661 cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa actgcaattg taagactttc
     3721 tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat agataatcat cgcttcctca
     3781 cataagccag ggttagtgac ctgctttctc ttcctgccag cgttcatgta actgattcag
     3841 acgggcgctg atcgctttcc agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc
     3901 tttatgatac aaacgtgtaa catgttctgc cttgatcggt gacagattat ctaacacgct
     3961 gacggcccct ttacgattat tgcagaccag gatcatatcg caacccgcat ccagtgatgc
     4021 ctgcccgcgt tcggcataac tgcccataat cgcggcacct tccatcgata aatcgtcaga
     4081 gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg gttttcagcc agtaggaaga
     4141 accgctcgcc ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc
     4201 gagtttattt tcgcggatta acgtgctgaa aaccgacata tctttggcac gaatttccgc
     4261 ttgcgggcgt gggtcgcacg gcgtctcttt gtgtgaatct gcagttactg caccgtgtcc
     4321 tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc atcccatcaa taaaccgact
     4381 ggcgattgcc agggcttttt ctgggtcggc atgataagaa cgctcgccaa tcgccgcgct
     4441 gatatgtcct acatccagca ctggcgcaaa gctaatatca atatccatag cgatcatttc
     4501 gctggccatc agccaacccg cctcttgcgc cagtttgccg ccctcttcca ttcccaacag
     4561 cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct tcgcgaaaac gctggacgcg
     4621 tccaccttcc tgatctaccg ccaccaccag atgattgcgc gatgctgcgc ggatctggcg
     4681 caccagttca cgtaactggg caggatcatg atagtttcgc gtaaagagaa tcagccctcc
     4741 caccagcgga tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc
     4801 caacattact ggacccacac tgctctcctt attgttttat taatagctgc cgccaggtgt
     4861 catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc
     4921 ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg
     4981 gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgt tcagtatttt
     5041 ccacccacac cgccgccagt tccagcgcga tatcaccatc tcctgcatac tcccagtcga
     5101 tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg
     5161 gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acattttaac attcgcagcc
     5221 aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca
     5281 gcgttattcg ccagccaaaa cgtggttgtt gatgtagata atacagcaag cctgccagtt
     5341 cgttggtatc cggcaaatac gtttttaccg cgccgggcag atagtcgact accatccagt
     5401 cacggagata taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat
     5461 actggcgcaa gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac
     5521 gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta aacgtcgtga
     5581 cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg ggattattgc
     5641 tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc
     5701 atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg
     5761 ccgacattgc gggcaatgcc tatggcttta ctacgggtgc ctaaactatc ctgcggcgac
     5821 aaacctaact gttgcttagc catcgacagc tgctgggcgg aaaccagggt aaatttcccg
     5881 ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta
     5941 gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacccc gtcagcccca
     6001 agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatggcg aatatgcggc
     6061 gcaggcggtg cagtttgatc ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc
     6121 accgagggca ctgtggggac aggctgttgt ggctcggctg gttgttccgg cgctggtttc
     6181 acttcttcta ccggtgcagg ttcacgttgc cccacacacc cggcgagaaa catcgccagc
     6241 gcggtaatca aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag
     6301 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga
     6361 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
     6421 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt
     6481 tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc
     6541 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
     6601 atgtgaacga cagcccacca gcagtaacaa cgccagagac atcagcccaa agcatccttt
     6661 tctcatcgtt acagaccttt atgcgccagc attggtccca gcggacgacc acccaacaag
     6721 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat gatcagacga
     6781 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
     6841 cccagcgcct gctcatgctc agctgagacg tcgttcacag tcggaatgag gatattcgga
     6901 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
     6961 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
     7021 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
     7081 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt
     7141 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
     7201 aatgcgctga aaagacattc tctgtttggt ttacacatca aatatgaatg cgtatatttc
     7261 tcatttgcat ttacatacaa aattattcgc acactaaata taaccgctca tcaccctcct
     7321 ccggcatgag cctgacgaca acataaacca agagatttca aatgctttca acacaattta
     7381 acagggataa tcaatatcaa gccatcacca aaccgtcact acttgccggt tgcatagcat
     7441 tggcactatt accttctgcc gcttttgctg caccagccac tgaagaaacg gtgattgttg
     7501 agggttcagc cacagctcca gatgatggcg aaaatgatta cagcgtaacg tctacctctg
     7561 cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc
     7621 agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg
     7681 ggatcagcaa aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga
     7741 tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg
     7801 acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggac
     7861 tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca
     7921 gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg
     7981 tggcggattt acaaagccca ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg
     8041 gctaccagaa taacgactca tggctggacc gctacaatag tgaaaagacc ttcttctccg
     8101 gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac gaatatcagc
     8161 gcattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact gatggcagca
     8221 gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga
     8281 tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacactga
     8341 atgccaccca ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa
     8401 acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggc tttgattatg
     8461 tcggcggcac cggttggaac agtggcaaac gtaaagttga tgcgctggat ttgttcgctg
     8521 acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca
     8581 aacaaaacaa tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt
     8641 tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcgcagg
     8701 acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc
     8761 tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca
     8821 tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt
     8881 cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca
     8941 aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga
     9001 atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt
     9061 ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga
     9121 cagtcagtaa aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga
     9181 catttggcgc aacgcgctat attgcagagg ataacgaagg aaacgccgtt aatcctaatc
     9241 tgccacgcac cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga
     9301 cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg
     9361 gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg
     9421 tgacgaaaaa cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata
     9481 ccaacgtgga aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt
     9541 atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag tctccccaac
     9601 gtcttacaga ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt
     9661 tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt
     9721 tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa
     9781 tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg
     9841 ccatttcgct ggtacctgtt gcttttgcat cttcagtcgc atcttcacga cccggcgttt
     9901 tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga
     9961 taccgacgat cgggaacaac cacagtttgc tgctgttacc agacagaaca atgaagtcga
    10021 tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg
    10081 ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaaggaga
    10141 actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac
    10201 cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg
    10261 gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg
    10321 ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa
    10381 tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt
    10441 cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga
    10501 aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga
    10561 tggcagccag gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact
    10621 caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca ccggagataa
    10681 tccctccgag tacgccagta tccgccaggt gtttagaggc gatttcttca gcaggtaaat
    10741 gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg
    10801 cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa
    10861 tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg
    10921 cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggcagta
    10981 cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa
    11041 acataattga gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg
    11101 ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa
    11161 ataattcagt gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag
    11221 ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc accgcccaat
    11281 tttcggcggt gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc
    11341 aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg
    11401 ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt
    11461 cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg caccggcgca aggtaaggtg
    11521 agtcagtttc caccagtaac cgatccaggg ggacataacg cgcagcatcg cgcagttgct
    11581 ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt
    11641 tgcccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt
    11701 tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtatgg acgataaccg
    11761 gcttgttcag ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt
    11821 tagtttccgg tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt
    11881 ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca
    11941 cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atgccgcatg tgtaaataac
    12001 ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca
    12061 gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg
    12121 agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac aacgcccggt
    12181 tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct
    12241 gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc
    12301 aagggagaaa gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc
    12361 tgcgcagcac catgatggcg ttttagcgca tccatcagca acgttgccag ccagtgcaaa
    12421 cgcgccggag cttgttcatg attaagggcc gccagcagcg aataccagtc gcccgatggc
    12481 acgctatatg ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac
    12541 aacgccagtg ccgcgccagg cgagccggcg cttaagcgca atgcggcaag tagtgcatcc
    12601 tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg cggcggcgca
    12661 aggtaatgta accgacaacg actacgtaat gttgccagta aacgttcagg ctcgcgggta
    12721 gccaggaaaa accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc
    12781 gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaagcgt
    12841 gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag cgtatttttc
    12901 cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat
    12961 ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc
    13021 agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg
    13081 tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga
    13141 taccatctca tgcgtccaac tccttcaccc agtgggtcac ggtagtgcgg attgcatcca
    13201 tcacggcctc cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca
    13261 gttccagata gcgggcgcgg gtgcgattaa aaaaatcgaa agactcttgc tcaatacgat
    13321 ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga
    13381 gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt
    13441 ggtcaatacc acgtccgccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa
    13501 tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg
    13561 cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga
    13621 tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga
    13681 aaaccatgtc gcggataccc agttgctcga gcgtctcaac caccacgtta cgcgcggtag
    13741 ttttgcctgc gccttccaac ccctcaatga cgatatactt actgcgcatt tttttcctta
    13801 agcactttca gataatcctg cacagacttg ttatgacttg caagattggt attaaacgtg
    13861 tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca
    13921 gccttcagcg aatccgcccc cggcgtcgct atcgcacccg gcggcaaacc ggtaatggta
    13981 taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc
    14041 tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg
    14101 ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata
    14161 attgatgcca tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct
    14221 tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca
    14281 tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc aatccactcc
    14341 gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct cagcgtgtgc
    14401 ttgatatacg gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc
    14461 agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg
    14521 gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata
    14581 cgcagcaacc attgaaaaac ccgtgggcga ttgatgatct tatcggcata aagctgttca
    14641 cctagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc
    14701 aatttgctgt cggcaagatg gcgaaccttc cagatgccca caccagcggc gatacccagt
    14761 accaccagca ataacaagat tatcaataac acttttttca tgactaattc gggcgctcac
    14821 aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg ccacaggcac
    14881 gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct
    14941 cttccagaga ggcttgcact tcgacaagct gataagagga ttgtgccagc aaacggatac
    15001 agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacgt
    15061 tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa
    15121 ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcgaatcaat acttgctcaa
    15181 gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta
    15241 gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg
    15301 agagaatccg cgttgccggt ccactgttca atgtgctgta ccctcgcccg ccactaccgc
    15361 gactgatcac gactttcagt acaccatttt gctgttctgc tgccagcgtt ttcatctctt
    15421 gttcaagttg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc
    15481 gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac
    15541 aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttataaccgt
    15601 taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac
    15661 ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg
    15721 gaaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa
    15781 ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc
    15841 cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg
    15901 cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt
    15961 catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggttttcac
    16021 cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc
    16081 aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc
    16141 tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac
    16201 gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag
    16261 cataccggca ccatcgccca gtacgaagcc atcacgctct ttatcccacg ggcggctcgc
    16321 cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacacc
    16381 cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc
    16441 gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga
    16501 gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat
    16561 cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg
    16621 gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt
    16681 tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat
    16741 tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca
    16801 gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc
    16861 gattaggctg atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt
    16921 gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt
    16981 cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca ggcggtcgaa
    17041 cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa tggcagcctg
    17101 aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact cttcttccag
    17161 agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc
    17221 attgttagta acttcttcct gcttaacgcc cagctgttcg ccgataattt tcttaacgcg
    17281 ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt
    17341 cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt accacgattt
    17401 tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta attattactc
    17461 ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc agaccatgta
    17521 catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag
    17581 gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc
    17641 caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt caataaagcc
    17701 cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca gtgatttact
    17761 gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat ttcccatggt
    17821 accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca tagcgcgcat
    17881 taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat cgttccactc
    17941 ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat tgaccaggat
    18001 atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag atgccgggtc
    18061 ggtcacattc aacatcagac ctttgccgtt agcacctaaa taatcactga tcgcctgagc
    18121 gccattttca ctggtcgcag tgccaataac tttcgcgcca cgggctgcga gcgtttcagc
    18181 aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt
    18241 catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc
    18301 cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc
    18361 gacttcatag agatgttcta cgccttgcgc tgccatgtac tcaacagact tcgtccactg
    18421 aaccgggtta tacaactgac gtaccagtgc gtcacgaatg gcatcaccat tggtttcgca
    18481 tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa
    18541 ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg gtacgctcac
    18601 tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag cacgctcaac
    18661 cgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta ccggagaaac
    18721 gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat ccagaccgat
    18781 gattgccgcc atagcgcccg taccttccgg tacggcttct tgcatgaact tgccgcgcat
    18841 ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca gcgcggagta
    18901 ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta ccgccctgct gctgccatac
    18961 gcggtacagc gcaacagagg cagtcaacag cgctggctgg gtttgccagg ttttattcag
    19021 ttcttcagct ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc
    19081 ttcagcaaac gtttcttcga caattggata gctcgccgcc atatcagcca gcattccaac
    19141 ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc
    19201 ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt caagcagaac
    19261 caactgcccc ggcttaatgc gcccgtcgcg tacagcttca tccagcgcgc acgggacaga
    19321 ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca tcgacatacc
    19381 gagttttttc gccgttgcac tgataatacg caagttagcc tgatgcggaa ccagccagtc
    19441 cagttgagaa cggtcaagat tattcgccgc cagcgtctca tcaacgatgt gcgccagttc
    19501 cgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg aattctctgg
    19561 attcacacgg tcagcattag gcagcgtcag caactcacca tagctaccgt cggcatgcag
    19621 atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc ccgcgccatc
    19681 gccaaaaata ataatagtcc cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga
    19741 accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg ctacgcttaa
    19801 tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc
    19861 cagcatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc
    19921 cacaacgatc aggccaatct ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc
    19981 cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat
    20041 accggtacga gtgacaatcc actcgtcaga ggtgtccacc attttttcca aatcggcgtt
    20101 tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat acatgtacgc
    20161 tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg gcagcgtcct
    20221 gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct gggtatacag
    20281 attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt
    20341 caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc accgtgccgc
    20401 gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg aatcgcctcg
    20461 tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt tttttccctt
    20521 caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc gtctttaatg
    20581 tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt aacaactcat
    20641 tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc gctgaggcat
    20701 cccgaatact gtcgagcccc tttacttctt cttcaccaat attgagcaac gccacgcgag
    20761 gattgggaat ttccaccacc tcttcagcca gaactgagcc cataatggca aattgcacca
    20821 acattgtgct gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt ttgccctttt
    20881 gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg ggtttgagta
    20941 ataattttgc cagccccatc agtgccccgg tattaccggc actgacacag gcttgcgctc
    21001 gaccttcttt caccagctcc agggccacgc gcattgaaca cccacgactg gcgcggatag
    21061 cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc tgcagacgcg
    21121 aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga ttgccgacta
    21181 aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg
    21241 aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc aaggtatcgt
    21301 cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa gcttcatcac
    21361 gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat
    21421 gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg tgactgcggt
    21481 cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat tctgttgtac
    21541 ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga gcaccaatta
    21601 cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt cttcaggcag
    21661 ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat gcaccggaac
    21721 taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga tttcaccgaa
    21781 ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt cgtctgaacg
    21841 cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg
    21901 acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac gttggttatc
    21961 gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg attcggcgac
    22021 gcgctcaacc tgatcagggg tatagatacc ctggtaatca aggcgttttt gagccgtacg
    22081 aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat attaactttg
    22141 taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa taattcgcac
    22201 acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca acctgcaaaa
    22261 cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga aaaaaatatg
    22321 cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga aaaactgcaa
    22381 atctcttttg aatgtgcagc acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg
    22441 cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg
    22501 gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat caccggtaaa
    22561 ccgttaacgg aagaaaatac ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc
    22621 ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctgcaaac agaagtggag
    22681 ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta cgtgcgtaag
    22741 gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat tacgctgttt
    22801 gagcgtttag aggggcgtga ccctaacacg ctggttggtt taccgctaat cgcgctgtgt
    22861 cagatgttac gtcgggaagg gaaaaatccg ctgatgggat agttttttgc gccagatgcg
    22921 gttacgtcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg ccttatcagg
    22981 ccaaccgccc attatcaggc ttatatgctt gtattagcgc gcgttacgca gcttttgcaa
    23041 acaacgcttc aaaccatcat ccatcggcgc ttcgatacgc atcacctcac cggtccccgg
    23101 atgggtaaac ttcaacgctg cggcgtgcag gaacagacga tttaatcccg tgcctgcttc
    23161 agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc
    23221 cgcatactgt gtatgcacac ggatctgatg agtacgtccg gttaccgggc tacagcgcac
    23281 cagggtggca aatgcatagc gttcttccac tttaaagcgt gtttccgacg gtttgccttc
    23341 ctgactcaca cgcacgatac gttcgccgct ttgcagaata tttttcagta acggcgcttg
    23401 aacgctcttc acatgcgact gccactgacc gcgcaccagc gccaggtaat ctttttgcat
    23461 ccctttttca cgtaattgct catgcagaga acgcaacgcc gagcgttttt tcgctaccag
    23521 caatacacct gaagtgtccc ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg
    23581 gcgcaacgcc cgcaaacctt cgataacgcc gaagcttaaa ccactgccgc catgtaccgc
    23641 cgtaccggaa ggtttattca gcaccaggat atgatcatct tcatataaga tgacgtccgc
    23701 cagcgccgcc accttttgca gatgtggtga aaccgcctct tcttcccgct cagcaacgcg
    23761 aaccggtggg atacgcacct catcacccgc ttcgagttta tattcaggct taatacgttt
    23821 tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa atcatacttt ttggtacgcc
    23881 tttcaattgg gtacgcaaaa agttatcgat acgttgcccc gcttcgtcag cggtgatagc
    23941 aacaattttt acggatggag tctctgtttt catggttggc gattctaatt agccaacagg
    24001 attcgcgcca ctcatttttc tatgcttata tttacttttg caccttatta cttcactgcg
    24061 tgatcacttt attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt
    24121 aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga
    24181 ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc
    24241 cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg
    24301 ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg
    24361 tttctttgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct
    24421 ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca
    24481 cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag
    24541 ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg
    24601 tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg
    24661 caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg
    24721 attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc
    24781 ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg
    24841 aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct
    24901 ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggca
    24961 tttctcgccg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcaagccttg
    25021 aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg
    25081 cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg
    25141 aaagccgccc ggctccgttc ctgattcatc aggagagtaa cgtaatcgtt cgcgcattcc
    25201 gcgattactt acgccaggac atcggcgaaa tccttatcga taacccgaaa gtgctcgaac
    25261 tggcacgtca gcatatcgct gcattaggtc gcccggattt cagcagcaaa atcaaactgt
    25321 acaccggcga gatcccgctg ttcagccact accagatcga gtcgcagatc gagtccgcct
    25381 tccagcgtga agtccgtctg ccatccggtg gttcgattgt tatcgacagc accgaagcgt
    25441 taacggccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg
    25501 cgtttaacac taacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc
    25561 tcggcggcct gattgttatc gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg
    25621 tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata
    25681 tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat
    25741 ccagtcatca cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc
    25801 tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag
    25861 ttcacgccat tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg
    25921 taaatgccat tgaaactcgt caggacggtg tgcgctgtgt aattgtgcca aacgatcaga
    25981 tggaaacccc gcactaccac gtgctgcgcg tgcgtaaagg ggaggaaacc ccaaccttaa
    26041 gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg
    26101 ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt tgccatgccg gatgtgccgc
    26161 ctgcgccaac gccagctgaa cctgccgcac ctgttgtagc cccagcaccg aaagctgcac
    26221 cggcaacacc agcagctcct gcacaacctg ggctgttgag ccgcttcttc ggcgcactga
    26281 aagcgctgtt cagcggtggt gaagaaacca aaccgtccga gcaagcagca ccgaaagtag
    26341 aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc
    26401 gtaatgagcg ccgcgacacc cgtagtgaac gtgcagaagg cagcgataat cgcgaagaaa
    26461 accgtcgtaa tcgtcgccag gcacagcagc agactgccga gacgcgtgag agccgtcagc
    26521 aggctgaggt aacggaaaaa gcacgtacca ccgacgaaca acaagcgccg cgtcgtgaac
    26581 gtagccgccg ccgtaacgat gataaacgtc agacgcaaca agaagcgaag gcgctgaatg
    26641 ttgaagagca atctgttcag gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc
    26701 gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta gccgaagaag
    26761 cggtagtcgc accggtggtt gaagaaactg ccgctgccga accaattgtt caggaagctc
    26821 cagctccacg cacagaactg gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag
    26881 aacagcaaga agagaacaat gctgataacc gtgacaacgg tggcatgccg cgtcgttctc
    26941 gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc
    27001 gttatccaac ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg gaactggcct
    27061 ctggcaaagt ctggatccgc tatccaattg tacgtccgca agatgtacat gttgaagagc
    27121 agcgcgaaca ggaagaagca caagtgcagc cgatggtgac tgaggtacct gtcgccgccg
    27181 ctgtcgaacc tgttgttagc gcgccagttg ttgaagaagt ggctgaagtc gtagaacccc
    27241 ccgttcaggt tgccgaaccg caacctgaag tggttgaaac gacgcatcct gaagtgattg
    27301 ctgccgcggt aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg
    27361 aagttgcaga acacgcagaa ccggtggttg aaccgcagga agagacggca gatattgaag
    27421 aagttgccaa aactgctgag gttgtagttg ctgaacctga agttgttgct caacctgccg
    27481 cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt agctgcggtc gaacctgagg
    27541 tcaccgttga gcataaccac gctaccgcgc caatgacgcg cgctccagca ccggaatatg
    27601 ttccggaggc accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag
    27661 gtgccgcagg tggtcatacg gcaacacatc atgcctctgc cgctcctgcg cgtccgcaac
    27721 ctgttgagta ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat
    27781 ttcatctttg aatcaacata ttaaaattca tcgaatggca tccttgctaa ccaacaatgc
    27841 aaaataggca aaaaaatgcc cgccccggcg atcccagagc aggcagacaa aacataccca
    27901 gtttcatgat atgttttaaa gcgaaattat ttgttgagct ggaagagcga caatccctgc
    27961 atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa
    28021 atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaatgcg
    28081 cgatcgctac ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg
    28141 cgaacggtca gcacattgtt cagcgagttt ttcagtccgc ggttggtttt atctaacgcc
    28201 gcagcggcgg tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg
    28261 gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc
    28321 gcgttactgg taatactgtc gaaaattttg tcacccgtgt gccctatcac catcgaacgc
    28381 gaagcatcga cctgttgctt aatactttct gctccaccga cgtatttccc tttctcttcg
    28441 ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg
    28501 gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc
    28561 cggtcatcgt cactcaaagt gccattgctg gcgtagacaa ttttttcctg agcattctgg
    28621 atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa
    28681 gtacgtgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac tacggcttgt
    28741 gatgctgcaa tgggatcgtc agaagggtta acgactcgct tacctgtcga catctgctcg
    28801 ccgtacttca tccattctgc ctgagaattg gtgatgccac gcatgttttg ctggtacatc
    28861 atctgggtac tgaaacgcat cttatctccc cttagcgaat gttaatcagc gcatcaaaaa
    28921 tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca
    28981 gatttccgta ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct
    29041 gcgtcaccaa attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga
    29101 tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac
    29161 tgttgctttg cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt
    29221 cttcgctcgc catcgctatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg
    29281 cgtcacttac tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca
    29341 actccagacc atcaaatgcc actttaccgt tggcatctgg tgtcaccgta aaagtggtat
    29401 tgctggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg
    29461 ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc aacgttaccg ttgtttttag
    29521 tgttttgcag aaccgcgggc ttaccgatag caaagaaatc ttcaccctca tcgccgttgg
    29581 catcaaatcc ggctttgtgt tggctgttga aagcctcagc aaatgccagc gccagttgtc
    29641 caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg
    29701 acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacataag
    29761 cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac
    29821 ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg ccatcctgaa
    29881 cgctgacttc tacaccaaca atctggttta attcgctcac cagttgatcg cgttgatcca
    29941 gcagattgtt aggtgacgcc cctgccccca cgcctgtcag acgcgagatt tgatcgttca
    30001 ggctggcaat ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat
    30061 tgacctgttt gtcctggtcg cgaagatact gatcggtggt tttaaactga ttcaccaacc
    30121 cttctgattt cccaatcagc acctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg
    30181 tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga
    30241 gcatattgtc gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct
    30301 gcgccgcacg taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt
    30361 agacgccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag
    30421 tagtttggcg ggtatatccg gcaacgttat agctggagat attattactt gctgtattta
    30481 acgccgcctg ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg
    30541 ttccttataa gccttcaatt acagagtatt atcggcagcg ccccgccgga cttgagttat
    30601 tcagaacaga ttatcaatgt tcatactgta ggttttgctc accttgtcgc ttatcgattt
    30661 catctgctga atcatgttgg tgagtttacg ggcatagtga ggatcggtgg catagcccgc
    30721 gtcctgtagg gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg
    30781 cgggttacgt gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac
    30841 ccgaaacttc gcttttactt tcttcgcttc gccattttca tattcagtcg tggtgatttc
    30901 agtgactggc cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc
    30961 gccgttttcg cggcggattt gccgttgccc ccagccagat tccagcgccg cctgagcgag
    31021 gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg
    31081 cgcgaggaat gctttactgt cacctggcag cgaatcatcg tagttacgtg gcacggcctt
    31141 ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc acggtttcga gcgggaattt
    31201 catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgcttaac
    31261 catcatctct gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc
    31321 atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaaagc
    31381 gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg
    31441 acggatattt gccgccggat cttcgctcgc tttcgccttt agttcgttga gtgattgcgc
    31501 atcccaggcc gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt
    31561 gcccgcagac atcccgcact ttgcattgat tgcaaaatag acatcagatc catcggcgta
    31621 gcgcccagcg cattgagcgc acgcaccacg ttattgaggc tggcgctgga acgtacgctt
    31681 tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt
    31741 ccaccaccaa acggtgtatc tggctggctg acattggcct gacgattgac tgtaactgag
    31801 agattcccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa
    31861 ccggtgcgcg agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc
    31921 tggatatcgg caaggaaacg gacttgggaa ctgttgccac ttggtacgcg cacctgaata
    31981 gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg
    32041 atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg
    32101 ccaaactggc tgggtaattc acgttcaata accgcaccat tggtgatccg tccaccgttc
    32161 aattggttca cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata
    32221 ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggagt catcaacaac
    32281 gtacctccac gcaagctttt ggcattaccc atggaagaaa ccaccacgtc gatggtttgc
    32341 ccctgacgtc caaacggagg aagtgacgct gtcaccatta ccgcagcgac gttttttagc
    32401 tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt
    32461 tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga
    32521 ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc
    32581 tcagcctgag ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc
    32641 acagcctcac ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt
    32701 gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag
    32761 acggtacggt attgctgccg ctgatggtgc gtggattaac cacgccagag aagcgaataa
    32821 attcggtacc ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga
    32881 ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg gcattggccc
    32941 cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa
    33001 acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc
    33061 tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg
    33121 tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg
    33181 gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg
    33241 cactggtcgc cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa
    33301 gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct
    33361 tcaaaaatca ataaaccggt gaacctgcca ccgattatgc cttagagttg cgtcagtttt
    33421 tgcagcatct ggtcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc
    33481 tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga
    33541 tatagcaatc ccgcaccgtt caggcccggc gtgctttcgt tcggtgcacc ggaggattgc
    33601 gtttcggtgt agaggttttc gccaatgctc tccagcccgg tatcattcat aaaggtggtg
    33661 agattaagct gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg
    33721 ccatcacgac cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc
    33781 tgaaaaccac cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg
    33841 gtataggctg atgaaccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg
    33901 acatctttgc tgttgttggt ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg
    33961 accgggcgta cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac
    34021 tgtgcccccg gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc
    34081 ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc
    34141 tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg
    34201 gattagtgac aaagtgcata tgttttcttg ccggatgcgg cgtaaacgcc ttatccggcc
    34261 tacaagtgct cgcaaaatca ataaattgcg attgccccca tagacccgat aagcttgcgc
    34321 atcgggcaac ttatacttgt catgtatcct ttcaattaac tcatcgacag cagttggttg
    34381 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    34441 gcgctggcaa tcatgtcgct cattgccgca acggtattga cattactgcc ttccagaaca
    34501 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gcccacgcgt ggcctgggtt
    34561 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    34621 accagtttaa gacgccctac tggcgcaacc gtgtttgccg gatcgcccgg attcagcgcc
    34681 gagattgtgc catcggcagc aatagtgatt tccgcccctt ccggcacagc aattggcccc
    34741 gcctcgccta tcaccggatg cccctgaatt gtcagttgtc cggtgggatc aacctgaatg
    34801 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccagccatcc
    34861 tgctgcaatg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct
    34921 gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc
    34981 actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta
    35041 ctggcggtta ccgcctgttg attcagcgtc tggctggctg ctcccatcgc ggtataaatt
    35101 gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg tgttgaggat
    35161 ctggtcctgg gttttgatgg tctgggcgtt agactgatag ttacgctggg caacgatcat
    35221 attgaccagt tctttactga gatcgacgtt ggacgcttcc aacgcaccgt tggtcagggt
    35281 gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga
    35341 ccagacgttg ccgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat
    35401 ctgccccagc agttgggttt gttcgttgga atagttgccg acaaccgtac cgtcatcatt
    35461 gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatgtt
    35521 gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg tggcgggttc
    35581 tgcgccgtta attgcgccag ttgtaatatt ctctgttgga tttgacgtca gaacgccatt
    35641 ggcattaaac accagcgtca ttgcatggtc agctgtgcct gttggatcac tgctatcctg
    35701 ggtgtagacc tgccagttat tatccccggt cttcacaaag tacacgctca tgtcatgagc
    35761 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    35821 ggcatcaaat gcgttaacag agggaagcgg atcgctggaa ttcaggttga tctgcatcga
    35881 cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatgt tagtcggatt
    35941 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    36001 ttgcatattc accaggttac gattttcatc cagcttaaat tgtccgttac ggctgtagaa
    36061 caccgcaccg ttgctatcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    36121 accacgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt
    36181 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    36241 aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc
    36301 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    36361 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    36421 attaccgttg ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac
    36481 cagttgtgta ccaccgttac tggcgctaat cgctacgttg taagaaccgt tcggcgcagt
    36541 gctgccgtca gtcaacgtac cgtcccaggt gaaactgtgg actccggcgg tcagttcacc
    36601 aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc
    36661 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact
    36721 gccggttccc gcaagaacag tggtgccggg gatcatcacg ccgtgaccga tcaggttact
    36781 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag
    36841 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    36901 cattggattg gtcgggtcct ggtttttcag ctgcgccacc aacaaagtca gaaaactgct
    36961 ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    37021 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    37081 gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag
    37141 ctgcgtgacg ccgacatggt gttaaccatc tctccgacaa catcaacgtt cggcattttt
    37201 acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt gtccggggcc
    37261 tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    37321 acctgaaata ccacctgttt tgcccgatat ggctgtccat cgggaccggt cacgctatca
    37381 gcattcgcca gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgat
    37441 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    37501 atcatgcctt tgatttgccc gctcaacgca ctaaggctca tctggtattg caggctgtta
    37561 tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg
    37621 tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cggaatgtgt
    37681 tgcgttgagg tcatcgtcag agcaaccaca ctggttgcat cccgtccacg ttgcatgact
    37741 tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg
    37801 gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga
    37861 aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc
    37921 catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct
    37981 atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa atggctgggg
    38041 gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta
    38101 agtgcggcga gcaatctcac atcgcaattg caaacctttt ttagctccca gctcgcaggg
    38161 ataagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccgtgcgag
    38221 cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca
    38281 cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg
    38341 gttgccgcga tgcccattgt gcggggagga aagctggaag ctggcaatgt caaactaaaa
    38401 cgcggacggc tggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat
    38461 gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    38521 gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc
    38581 gccaacgctg aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc
    38641 atggtatctg gacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    38701 ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    38761 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcccgtaagc
    38821 accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acacccgggc ggcaaaaaca
    38881 acagcatcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    38941 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt
    39001 gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac
    39061 ttgcagagta attaatcgta tgacgcgtct tgcagagatc ctcgaccaga tgtccgctgt
    39121 gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat
    39181 caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg caacgttgga
    39241 ttacctcgaa cagttacgca ggaaagaacc caatacagca aatagcgttg atattagtca
    39301 acgctggcag gaaattgctg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    39361 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    39421 tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaacccatc gcggcagtaa
    39481 aaagatttcg atctgaagat tatctccggc ctgcactgca ggccggaatg cattgttaca
    39541 ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg
    39601 cggcaatccc cgccagcacg accgccatta aacgcagcaa gcgccagggc atggtaccca
    39661 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    39721 ccaccaccag acgcaacaga aacgccatcc agccaggttg cggggtaaag attttctgct
    39781 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    39841 acagcccggc atgtttcaac ggaccgataa acgccaggtt catcaattgc gttaaaatca
    39901 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    39961 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    40021 gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa acggtcaacg
    40081 gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa caaagacgca
    40141 gcccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    40201 gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggacgga aactccatta
    40261 agcggtcggc gtaatacatc cacgacaccg aaccggaagc aagaaacgag gcaaaaatgg
    40321 tgttgatgat taaggagatc tgactcacag agacgccaag gattgccggt cccatctgtt
    40381 tcaccacacg cattgctccg gcatcgtgga agttaatgcg tggcaaaacc agcatgccga
    40441 tcttcttcag gtgcggtagc tgatacacca gctgcaggac gccgccgacc gtaacagccc
    40501 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    40561 tgctgatgtt aagcagtgtt ggagcaaacg ccggaatcga gaagcggttc cacgtattca
    40621 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta
    40681 gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca
    40741 tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcgagtgtca
    40801 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat
    40861 attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta
    40921 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    40981 ttctggcgac aattgcgtca cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    41041 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc cacgggcaaa
    41101 acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg gtgaattgct
    41161 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    41221 cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt ttgcacacat
    41281 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagcgtgct ctgccaacca
    41341 ggaatcggtt tattcactac accctgccca cgctcctcac gccattcgcg catttccgta
    41401 atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg
    41461 cgatgcatac aggtggtgat ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc
    41521 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac
    41581 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    41641 tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc ggtggcgagt
    41701 tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    41761 agttttttcc gcgccgccag ttccaccagc cgttcagcat cgcgcagatt ttctgccagc
    41821 ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtga
    41881 ctggcggtgc tggaatgtac aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa
    41941 tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac
    42001 caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    42061 gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat ctcccaggtg
    42121 ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca gggcttcgac
    42181 gcgggcctgt aaatcaccgt caaccgcgct cgacatatcc gttaccgacg gctgatcctc
    42241 tacctcaccg ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag
    42301 acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtca actccacttc
    42361 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg
    42421 ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca gtttcagatc
    42481 gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct
    42541 caccgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca cttcagattc
    42601 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtta ctacaccatt
    42661 gactgagagc ggatattgct ccggcgtggt cacctgcttt tccagcaagc agccaatcac
    42721 gcgggcttcc agagcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    42781 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    42841 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctacgtt
    42901 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac
    42961 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    43021 aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc
    43081 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    43141 gaaattcgtt aatcatcccc agcctggcct gccagcctga tggatagcag tggctttcgt
    43201 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgcaagac
    43261 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    43321 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    43381 tacgctgtaa atattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat
    43441 tgtctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    43501 aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    43561 tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca ggcgaggaac ctgggtaaat
    43621 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    43681 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgctctcg
    43741 gtctacgcca atttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct
    43801 ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga ttcgccacaa
    43861 tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggcg
    43921 gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacaac
    43981 gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc attggcgcat
    44041 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggcagttt
    44101 tgtttgtact gtgtgcagcg ttcaatgcgt ggttattacc ggcatggaaa ctctccaccg
    44161 tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct
    44221 atgttctgac gctggcgggt tactacatgc tggctgttca agtgatgctg atgctgccaa
    44281 ttatggtcaa cgacgtggcg ggcgcgccct ctgccgttaa atggatgtat gccattgaag
    44341 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    44401 tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca
    44461 tggtcagcgg cctgcaacaa cttttcaccc tgatttgttt gttttatatc gggtcgatca
    44521 ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca
    44581 gctatatggg gtttagccgt ctgggtctgg cgattggtgg cgctattggt tatatcggtg
    44641 gcggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc
    44701 tgggcattat tggcatcttc actttccttg cgctgggttg gcagtttagc cagaaacgcg
    44761 ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    44821 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    44881 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac
    44941 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    45001 ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg
    45061 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgca attgaagagt
    45121 ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg
    45181 catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca
    45241 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    45301 atgatttacg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    45361 cggaagatga tattcagcta ttcccgctac tgcgtaatct gacgctggta gccggaatta
    45421 actggccaag ccacgttgct gattaccgcg ataatatggc gaaacagaca caaatcaatt
    45481 tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc
    45541 cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt
    45601 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    45661 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    45721 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta
    45781 cccggcgtgg ctgacgccca tattgttctg acaaacctga ccagccaaat tggtcgcgaa
    45841 gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    45901 agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    45961 ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg
    46021 caaacggtga tgcaaacgtt gcttccctat ctgaaccagg cattacgcaa ttactttaac
    46081 cagcaacctg cttacgtcct gcgcgaagat ggcagccagg gcgaagcaat ggcgaaaaaa
    46141 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccatttac tgattaatca
    46201 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    46261 gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag
    46321 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    46381 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    46441 gctccgcccg tgaccaccat tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc
    46501 gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga ttcgctggat
    46561 cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca ccgctgcaaa actttacccg
    46621 gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat catgccggta
    46681 cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca
    46741 gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc
    46801 cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga tgctgccgac
    46861 tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt
    46921 aaccgcaacc atatgctggt tggaggcgtg cgtccgcacc tgtattgtct acccatcctc
    46981 aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgagta
    47041 ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag ttgcggctgc
    47101 gcgggctgct tcaacgcccc aaccgcgctg ggaagttacg ctaccgtctt tgaagagatg
    47161 aatgctctgc agcactttga agcattctgt tctgtaaatg gcccgcagtt ctatgggttg
    47221 ccggtcaacg acacattcat cgaactggta cgtgaagagc atcaggttgc tgaaagcatc
    47281 gcactgactg atgacacgct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt
    47341 aaacaataaa aatcgtagct tcctgttgtc attagattat tttacctgta taaataacca
    47401 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    47461 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    47521 tcctgataat gaaggccacg tatcggtacg ttacgccgca gcgaataatt tatcggttat
    47581 tggcgcgaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    47641 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc
    47701 ccggcttttt tattttactt acgaattttg agtacaaaat ttctacact
//
LOCUS       DAZUGK010000011        47218 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZUGK010000011 DAZUGK010000000
VERSION     DAZUGK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.011"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..311
                     /gene="yahO"
                     /locus_tag="ACHY5W_000548"
     CDS             36..311
                     /gene="yahO"
                     /locus_tag="ACHY5W_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFS4445510.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(409..1995)
                     /gene="prpR"
                     /locus_tag="ACHY5W_000549"
     CDS             complement(409..1995)
                     /gene="prpR"
                     /locus_tag="ACHY5W_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFS4445511.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            2234..3124
                     /gene="prpB"
                     /locus_tag="ACHY5W_000550"
     CDS             2234..3124
                     /gene="prpB"
                     /locus_tag="ACHY5W_000550"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFS4445512.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            3169..4338
                     /gene="prpC"
                     /locus_tag="ACHY5W_000551"
     CDS             3169..4338
                     /gene="prpC"
                     /locus_tag="ACHY5W_000551"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFS4445513.1"
                     /translation="MRDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWETKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            4372..5823
                     /gene="prpD"
                     /locus_tag="ACHY5W_000552"
     CDS             4372..5823
                     /gene="prpD"
                     /locus_tag="ACHY5W_000552"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFS4445514.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            5863..7749
                     /gene="prpE"
                     /locus_tag="ACHY5W_000553"
     CDS             5863..7749
                     /gene="prpE"
                     /locus_tag="ACHY5W_000553"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFS4445515.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTLWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            7985..9244
                     /gene="codB"
                     /locus_tag="ACHY5W_000554"
     CDS             7985..9244
                     /gene="codB"
                     /locus_tag="ACHY5W_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFS4445516.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALMFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            9234..10517
                     /gene="codA"
                     /locus_tag="ACHY5W_000555"
     CDS             9234..10517
                     /gene="codA"
                     /locus_tag="ACHY5W_000555"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFS4445517.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            10539..10636
                     /locus_tag="ACHY5W_000556"
                     /pseudo
     CDS             10539..10636
                     /locus_tag="ACHY5W_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10650..11549)
                     /gene="cynR"
                     /locus_tag="ACHY5W_000557"
     CDS             complement(10650..11549)
                     /gene="cynR"
                     /locus_tag="ACHY5W_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFS4445518.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            11659..12318
                     /gene="cynT"
                     /locus_tag="ACHY5W_000558"
     CDS             11659..12318
                     /gene="cynT"
                     /locus_tag="ACHY5W_000558"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFS4445519.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            12349..12819
                     /gene="cynS"
                     /locus_tag="ACHY5W_000559"
     CDS             12349..12819
                     /gene="cynS"
                     /locus_tag="ACHY5W_000559"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFS4445520.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            12825..14006
                     /gene="cynX"
                     /locus_tag="ACHY5W_000560"
     CDS             12825..14006
                     /gene="cynX"
                     /locus_tag="ACHY5W_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFS4445521.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(14109..14720)
                     /gene="lacA"
                     /locus_tag="ACHY5W_000561"
     CDS             complement(14109..14720)
                     /gene="lacA"
                     /locus_tag="ACHY5W_000561"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFS4445522.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(14786..16039)
                     /gene="lacY"
                     /locus_tag="ACHY5W_000562"
     CDS             complement(14786..16039)
                     /gene="lacY"
                     /locus_tag="ACHY5W_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFS4445523.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(16091..19165)
                     /gene="lacZ"
                     /locus_tag="ACHY5W_000563"
     CDS             complement(16091..19165)
                     /gene="lacZ"
                     /locus_tag="ACHY5W_000563"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFS4445524.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPIAVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTVPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQLFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISTWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YWIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLLWCQK"
     gene            complement(19288..20370)
                     /gene="lacI"
                     /locus_tag="ACHY5W_000564"
     CDS             complement(19288..20370)
                     /gene="lacI"
                     /locus_tag="ACHY5W_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFS4445525.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(20407..21360)
                     /locus_tag="ACHY5W_000565"
     CDS             complement(20407..21360)
                     /locus_tag="ACHY5W_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFS4445526.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(21380..22156)
                     /locus_tag="ACHY5W_000566"
     CDS             complement(21380..22156)
                     /locus_tag="ACHY5W_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4445527.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(22271..23104)
                     /gene="mhpR"
                     /locus_tag="ACHY5W_000567"
     CDS             complement(22271..23104)
                     /gene="mhpR"
                     /locus_tag="ACHY5W_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFS4445528.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCWEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            23295..24959
                     /gene="mhpA"
                     /locus_tag="ACHY5W_000568"
     CDS             23295..24959
                     /gene="mhpA"
                     /locus_tag="ACHY5W_000568"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFS4445529.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            24961..25905
                     /gene="mhpB"
                     /locus_tag="ACHY5W_000569"
     CDS             24961..25905
                     /gene="mhpB"
                     /locus_tag="ACHY5W_000569"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFS4445530.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            25923..26789
                     /gene="mhpC"
                     /locus_tag="ACHY5W_000570"
     CDS             25923..26789
                     /gene="mhpC"
                     /locus_tag="ACHY5W_000570"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFS4445531.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            26799..27608
                     /gene="mhpD"
                     /locus_tag="ACHY5W_000571"
     CDS             26799..27608
                     /gene="mhpD"
                     /locus_tag="ACHY5W_000571"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFS4445532.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGCGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            27605..28555
                     /gene="mhpF"
                     /locus_tag="ACHY5W_000572"
     CDS             27605..28555
                     /gene="mhpF"
                     /locus_tag="ACHY5W_000572"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFS4445533.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLETNVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEVVQAYVPGYRLKQCVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            28552..29565
                     /gene="mhpE"
                     /locus_tag="ACHY5W_000573"
     CDS             28552..29565
                     /gene="mhpE"
                     /locus_tag="ACHY5W_000573"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFS4445534.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            30042..31253
                     /gene="mhpT"
                     /locus_tag="ACHY5W_000574"
     CDS             30042..31253
                     /gene="mhpT"
                     /locus_tag="ACHY5W_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFS4445535.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            31355..31894
                     /gene="yaiL"
                     /locus_tag="ACHY5W_000575"
     CDS             31355..31894
                     /gene="yaiL"
                     /locus_tag="ACHY5W_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFS4445536.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(32019..32852)
                     /gene="fghA"
                     /locus_tag="ACHY5W_000576"
     CDS             complement(32019..32852)
                     /gene="fghA"
                     /locus_tag="ACHY5W_000576"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS4445537.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATTKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(32945..34054)
                     /gene="frmA"
                     /locus_tag="ACHY5W_000577"
     CDS             complement(32945..34054)
                     /gene="frmA"
                     /locus_tag="ACHY5W_000577"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFS4445538.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(34089..34364)
                     /gene="frmR"
                     /locus_tag="ACHY5W_000578"
     CDS             complement(34089..34364)
                     /gene="frmR"
                     /locus_tag="ACHY5W_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFS4445539.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(34524..35570)
                     /gene="fbpC"
                     /locus_tag="ACHY5W_000579"
     CDS             complement(34524..35570)
                     /gene="fbpC"
                     /locus_tag="ACHY5W_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFS4445540.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWPGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(35582..37660)
                     /locus_tag="ACHY5W_000580"
     CDS             complement(35582..37660)
                     /locus_tag="ACHY5W_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4445541.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(37729..38760)
                     /locus_tag="ACHY5W_000581"
     CDS             complement(37729..38760)
                     /locus_tag="ACHY5W_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4445542.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(38757..40073)
                     /gene="uhpC"
                     /locus_tag="ACHY5W_000582"
     CDS             complement(38757..40073)
                     /gene="uhpC"
                     /locus_tag="ACHY5W_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS4445543.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(40146..41687)
                     /locus_tag="ACHY5W_000583"
     CDS             complement(40146..41687)
                     /locus_tag="ACHY5W_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS4445544.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQVGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(41687..42316)
                     /locus_tag="ACHY5W_000584"
     CDS             complement(41687..42316)
                     /locus_tag="ACHY5W_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4445545.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            42619..43581
                     /gene="tauA"
                     /locus_tag="ACHY5W_000585"
     CDS             42619..43581
                     /gene="tauA"
                     /locus_tag="ACHY5W_000585"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4445546.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            43594..44361
                     /gene="tauB"
                     /locus_tag="ACHY5W_000586"
     CDS             43594..44361
                     /gene="tauB"
                     /locus_tag="ACHY5W_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFS4445547.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            44358..45185
                     /gene="tauC"
                     /locus_tag="ACHY5W_000587"
     CDS             44358..45185
                     /gene="tauC"
                     /locus_tag="ACHY5W_000587"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFS4445548.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            45182..46033
                     /gene="tauD"
                     /locus_tag="ACHY5W_000588"
     CDS             45182..46033
                     /gene="tauD"
                     /locus_tag="ACHY5W_000588"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFS4445549.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(46140..47114)
                     /gene="hemB"
                     /locus_tag="ACHY5W_000589"
     CDS             complement(46140..47114)
                     /gene="hemB"
                     /locus_tag="ACHY5W_000589"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFS4445550.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT        10847 a        12502 c        13032 g        10837 t
ORIGIN      
        1 gggtgatagt gtgataacaa ctctggagcc gtaatatgaa aataatctct aaaatgttag
       61 tcggtgcgtt agcgtttgcc gttaccaatg tctatgccgc tgaattgatg actaaagcgg
      121 aatttgaaaa agtcgaatcg caatatgaaa aaataggtga tatttcaacc agcaatgaaa
      181 tgtcgaccgc agacgcgaaa gaagatttga tcaaaaaagc ggatgaaaaa ggggctgatg
      241 tgttggtact gacctccggt caaactgaca ataagatcca cggcacggca aatatttata
      301 agaagaagta attctgaatc ctatgtaaac atccccgatg cgtaagttta tcggtgatca
      361 tctattgaaa tttatgccgg ataaagcgtt cgcgctgcat tcggcagttc agtttttcag
      421 ccgccgccag aacgtcgtcc ggctgatgcc taaataattc gccgctgctg ttttatcgcc
      481 tttaaatttc tccagtgcct gttgtggtgt cagcaagcgt ggagcgggaa ttttcgccga
      541 ctcgcgcgcc agttccggca gtagcagctg caaaaactgc ggcgttaaat ccggcgtcgg
      601 ttccacactt aaaaacagcg ccagtcgctc catcatattg cgcagttctc gaatattgcc
      661 cggccagtcg tagcgcagca gcacagtttc acttgcctgt aacccctggc gtaatgcagc
      721 agaaaatggg gcggagagcg ccgccagaga cactttcaaa aaactttccg ccagtggcag
      781 aatatctgcc acccgctcgc gcagtggtgg caattgcaga cgcaaaatac tcagccgata
      841 aaacagatca cggcgaaact gcccttgccg catatcttct tccagattgc agtgagtggc
      901 gctaatgacc cgcacatcca ccggaacagg ctgatgcccg ccgacgcggg tgacctcttt
      961 ttcttccagc acccgtaaca ggcgagtctg caaaggtagc ggcatttcgc caatctcatc
     1021 cagaaacagc gtaccgccgt gggcaatttc aaacagcccg gcacgacctc cgcgtcgcga
     1081 gccggtaaac gccccttcct catagccaaa cagctccgct tccagcagcg attcggcaat
     1141 cgccccgcag ttgacggcaa caaacggatg cgactttttg ccctgtcgcg catcgtggcg
     1201 ggcaaaatat tcccgatgaa ttgcctgggc cgccagctct ttgcccgtcc ccgtttcccc
     1261 ctcaatcaac accgctgcac tggagcgggc atacagcaaa atagtctgcc gtacttgttc
     1321 catctgtggt gattgaccga gcatatcgcc cagcacgtaa cgagttctca gggcgttgcg
     1381 ggtggcatcg tgagtgttat ggcgtaacga catgcgcgtc atatccagcg catcgctgaa
     1441 cgcctggcgc acggtggcgg cggaatagat aaaaattccg gtcattccgg cttcttctgc
     1501 cagatcggta atcagccccg cgccgaccac cgcttcggtg ccgttagctt ttagctcgtt
     1561 aatctgcccg cgtgcgtctt cttcggtaat gtagctacgt tgatcgaggc gcaaattaaa
     1621 ggttttttga aacgccacca gtgccggaat agtttcctga taagtgacaa cgccgataga
     1681 agaggtgagc tttccggctt ttgccagtgc ctgtaacaca tcgtagccgc tcggtttaat
     1741 caaaataact ggcactgaca ggcggctttt caggtacgcg ccgttagagc cagcggcgat
     1801 gatggcgtcg cagcgttcgt ttgccagttt cttgcggata taggtcactg ctttttcaaa
     1861 gccaagctgg ataggggtaa tgttcgccag gtgatcaaac tcgaggctga tatcgcgaaa
     1921 cagctcgaac aggcgcgtta cagataccgt ccagataacc ggtttgtcgt cattaagccg
     1981 tggtggatgt gccatagcgc accgcaaagt taagaaaccg aatattgggt ttagtcttgt
     2041 ttcataattg ttgcaatgaa acgcggtgaa acattgcctg aaacgttaac tgaaacgcat
     2101 atttgcggat tagttcatga ctttatctct aacaaattga aattaaagat ttaattttat
     2161 taaggcaatt gtggcacacc ccttgctatg tgtttatcaa cgcaaataac aagttgataa
     2221 caaaggatgg gctatgtctc tacactctcc aggtaaagcg tttcgcgctg cactgtctaa
     2281 agaaaatcca ttgcagattg ttggcaccat taacgccaac catgcgctgt tggcgcagcg
     2341 tgccggatat caggcaattt atctctccgg cggtggcgtg gcggcaggtt cgctggggct
     2401 gcccgatctc ggtatttcta ctcttgatga cgtgttgacc gatattcgcc gtatcaccga
     2461 cgtttgttcg ctgccgctgc tggtggatgc ggatatcggt tttggttctt ctgcttttaa
     2521 cgtggcgcgc accgtgaaat cgatgattaa agccggtgcg gcaggattgc atattgaaga
     2581 tcaggttggt gcgaaacgct gcggtcatcg tccgaataaa gcgatcgtct cgaaagagga
     2641 gatggtggat cggatccgtg cggcggtgga tgcgaaaacc gatcctgatt ttgtgatcat
     2701 ggcgcgcacc gatgcgctgg cggtagaggg gctggatgcg gcgatcgagc gtgcgcaggc
     2761 ctatgttgaa gcgggtgccg agatgttgtt cccggaggcg attaccgaac tcgccatgta
     2821 ccgccagttt gccgatgcgg tgcaggtgcc aatcctcgcc aatattaccg aatttggtgc
     2881 cacgccgctg tttaccaccg acgaattacg cagcgcccat gtcgcaatgg cgctgtaccc
     2941 gctttcagcg ttccgcgcca tgaaccgcgc cgctgaacat gtctacaacg tcctgcgcca
     3001 ggaaggcacg cagaaaagcg tcatcgacac catgcagacc cgcaacgagc tgtacgaaag
     3061 catcaactac taccagtacg aagagaagct cgacgacctg tttgcccgta gccaggtgaa
     3121 ataaaacaca acgaaaaata tctcgaccct acaaatgacg aggacaatat gagagacaca
     3181 acgatcctgc aaaacagtac ccatgtcatt aaaccgaaaa aatcggtagc actttctggc
     3241 gttccggcgg gcaatacggc gctctgcacc gtgggtaaaa gtggcaatga cctgcattac
     3301 cgcggctacg atattcttga tctggcggaa cattgcgaat ttgaagaagt ggcgcatctg
     3361 ttgatccacg gcaaactgcc gacccgtgac gaactcgccg cttacaaaac aaaactgaaa
     3421 gccctgcgcg gtttaccggc taatgtgcgt actgtgctgg aagccttacc ggcggcgtcg
     3481 cacccgatgg atgttatgcg caccggcgtt tccgcgctcg gctgcacgct gccagaaaaa
     3541 gaggggcata ccgtctctgg cgcgcgggat attgccgaca aactgctggc gtcacttagc
     3601 tcgattcttc tctactggta tcactacagc cacaacggcg aacgcatcca gccggaaacc
     3661 gatgacgact ccatcggtgg tcacttcctg catctgctgc acggcgaaaa gccgtcgcaa
     3721 agctgggaaa aggcgatgca tatttcgctg gtgctgtacg ccgaacacga gtttaacgcc
     3781 tccaccttta ccagccgggt gattgcgggc accggctctg atatgtattc cgcgattatt
     3841 ggcgcgattg gtgcactgcg cgggccgaag cacggcgggg cgaatgaagt gtcgctggag
     3901 atccagcaac gctacgaaac gccggacgaa gccgaagccg atatccgcaa gcgggtggaa
     3961 aacaaagaag tggtcattgg ttttggtcat ccggtttata ccatcgccga cccgcgccac
     4021 caggtgatta aacgtgtggc gaagcagctc tcgcaggaag gcggctcgct gaagatgtac
     4081 aacatcgccg atcgcctgga aacggtgatg tgggaaacga aaaagatgtt ccccaatctc
     4141 gactggttct ccgctgtttc ctacaacatg atgggcgttc ccaccgagat gttcacacca
     4201 ctgtttgtta tcgcccgcgt caccggctgg gcggcgcaca ttatcgaaca acgtcaggac
     4261 aacaaaatta tccgtccttc cgccaattat gttggaccgg aagaccgcca gtttgtcgcg
     4321 ctggataagc gccagtaaac ctctacgaat aacaataagg aaacgtaccc aatgtcagct
     4381 caaatcaaca acatccgccc ggaatttgat cgtgaaatcg ttgatatcgt cgattacgtc
     4441 atgaactacg aaatcagttc cagagtggcc tacgacaccg cccattactg cctgctcgac
     4501 acgctcggct gcggtctgga agctctcgaa tacccggcct gtaaaaaact gctggggcca
     4561 attgttccgg gcaccgtcgt acctaacggc gtgcgcgtcc ccggaactca gttccagctc
     4621 gaccccgtcc aggcggcatt taacatcggc gcgatgatcc gttggctcga tttcaacgat
     4681 acctggctgg cggcggagtg ggggcatcct tccgacaacc tcggcggcat tctggcaacg
     4741 gcggactggc tttcacgcaa cgcggtcgcc agcggcaaag cgccgttgac catgaaacaa
     4801 gtgctgaccg gaatgattaa agctcatgaa attcagggct gcatcgcgct ggaaaactcc
     4861 tttaaccgcg ttggcctcga ccacgttctg ttagtgaaag tggcttccac cgccgtggtc
     4921 gccgaaatgc ttggcctgac ccgcgaggaa attctcaacg ccgtttcgct ggcgtgggtg
     4981 gacggtcagt cgctgcgcac ctatcgccat gcgccgaaca ccggcacgcg taaatcctgg
     5041 gcggcgggcg atgccacttc ccgcgcggta cgtctggcac tgatggcgaa aacgggcgaa
     5101 atgggttacc cgtcagccct gactgcgccg gtgtggggct tctacgacgt ctcctttaaa
     5161 ggtgagtcgt tccgcttcca gcgtccgtac ggttcttacg tgatggaaaa tgtgctgttc
     5221 aaaatctcct tcccggcgga gttccactcc cagacggcag ttgaagcggc gatgacgctc
     5281 tatgaacaga tgcaagcagc aggcaaaacg gcggcggata tcgaaaaagt gaccattcgc
     5341 acccacgaag cctgtattcg catcatcgac aaaaaagggc cgctcaataa cccggctgac
     5401 cgcgaccact gcattcaata catggtggcg atcccgctgt tgttcgggcg cttaacggcg
     5461 gcagattacg aggacaacgt tgcgcaagat aaacgcatcg acgccctgcg cgagaagatc
     5521 aattgctttg aagatccgac gtttaccgct gactaccacg acccggaaaa acgcgccatc
     5581 gccaatgcca ttactcttga gttcaccgac ggcacgcgct ttgaagaagt tgtggtggag
     5641 taccccattg gtcatgctcg ccgccgtcag gatggcattc cgaagctggt ggataaattc
     5701 aaaatcaatc tcgcgcgcca gttcccgact cgccagcagc agcgcattct ggaggtttct
     5761 ctcgacagag ctcgcctgga acagatgccg gtcaatgagt atctcgacct gtacgtcatt
     5821 taagtaaacg gcggtaaggc gtaagttcaa caggagagca ttatgtcttt tagcgaattt
     5881 tatcagcgtt cgattaacga accggagcag ttctgggccg agcaggcccg gcgtattgac
     5941 tggcagacgc cctttacgca aacgctcgat cacagcaatc cgccgtttgc ccgttggttt
     6001 tgtgaaggcc gaaccaactt gtgtcacaac gctatcgatc gctggctgga gaaacagcca
     6061 gaggcgctgg cgctgattgc cgtctcttcg gaaacagaag aagagcgcac ctttaccttt
     6121 cgccagcttc atgacgaagt aaacgcggtg gcgtcaatgc tgcgctcgct gggcgtgcag
     6181 cgtggcgatc gggtgctggt gtatatgccg atgattgccg aagcgcatat taccctgctg
     6241 gcctgcgcgc gcattggcgc tattcactcg gtggtgtttg gtggatttgc ctcgcacagc
     6301 gtggcggcgc gaattgatga tgctaaaccg gtgctgattg tctcggctga tgccggagcg
     6361 cgcggtggca aaatcattcc ctataaaaaa ttgctcgacg atgcgataag tcaggcgcag
     6421 caccagccgc gccatgtttt gctggtggat cgcgggctgg cgaaaatggc gcgcgtcagc
     6481 gggcgggatg tcgatttcgc gtcgttgcgc catcaacaca tcggcgcgcg ggtaccggtg
     6541 gcgtggctgg aatccaacga aacctcctgc attctctaca cctccggcac gaccggcaaa
     6601 cctaaaggcg tgcagcgtga cgtcggcgga tatgcggtgg cgctggcgac ctcgatggac
     6661 accatttttg gcggcaaagc gggcggtgtg ttcttttgtg cttcggatat cggctgggtg
     6721 gtggggcatt cgtatatcgt ctacgcgccg ctgctggcgg ggatggcgac tatcgtttac
     6781 gaaggattac cgacctggcc ggactgcggc gtgtggtgga aaatcgtcga gaaatatcag
     6841 gttagccgga tgttttcagc gccgaccgcc attcgcgtgc tgaaaaaatt ccctaccgct
     6901 gaaattcgta aacacgatct ctcgtcgctg gaagtgctct atctggctgg agaaccgctg
     6961 gacgagccga ccgccagttg ggtgagcaat acgctggatg tgccggtcat cgacaactac
     7021 tggcagaccg aatccggctg gccgattatg gcgattgctc gcggtctgga cgacaggccg
     7081 acgcgtctgg gaagccccgg cgtgccgatg tatggctata acgtgcagtt gcttaatgaa
     7141 gtcaccggcg aaccgtgtgg cgtcaacgag aaagggatgc tggtggtgga agggccgctg
     7201 ccgccggggt gtattcagac cctctggggc gacgacgacc gctttgtgaa gacgtactgg
     7261 tcgctgtttt cccgcccggt gtacgccacc tttgactggg gcatccgtga cgctgacggt
     7321 tatcacttta ttctcgggcg cactgacgat gtgattaacg ttgccgggca tcggctgggg
     7381 acgcgcgaga ttgaagagag tatctccagt catccgggcg ttgccgaagt ggcggtggtt
     7441 ggggtgaaag atgcgctgaa agggcaggtg gcggtagcgt ttgtcattcc gaaagagagc
     7501 gacagtctgg aagatcgtga tgtggcgcat tcgcaagaga aggcgattat ggcgctggtg
     7561 gacagccaga ttggcaactt tggccgcccg gcgcacgtct ggtttgtctc gcaattgccg
     7621 aaaacgcgat ccggaaaaat gctgcgccgc acgatccagg cgatttgcga aggacgcgat
     7681 cctggggatc tgacgaccat tgatgatcct gcgtcgttgg atcagatccg ccaggcgatg
     7741 gaagagtaag gtattgtcgg atgcgtcgcg tggcgcatcc gacagttgtg cgcagatgcc
     7801 tgatgcgacg ctggcgcgtc ttatcatgcc tgcccactgt ttttacaccg ataattttcc
     7861 cccacctttt tgcactcatt catataaaaa atatatttcc ccacgaaaac gattgctttt
     7921 tatcttcaga tgaatagaat gcggcggatt ttttgggttt caaacagcaa aaagggggaa
     7981 tttcgtgtcg caagataaca actttagcca ggggccagtc ccgcagtcgg cgcggaaagg
     8041 ggtattggca ttgatgttcg tcatgctggg attaaccttc ttttccgcca gtatgtggac
     8101 cggcggcact ctcggaaccg gtcttagcta tcatgatttc ttcctcgcag ttctcatcgg
     8161 caaccttctc ctcggtattt acacttcatt tcttggttac attggcgcaa aaaccggcct
     8221 gaccacccat cttcttgctc gcttttcgtt tggtgttaaa ggctcatggc tgccttcact
     8281 gctgctgggc ggcacccagg ttggctggtt tggcgtcggt gtggcgatgt ttgctattcc
     8341 ggtgggcaag gcaaccgggc tggatattaa tttgctgatt gccgtttccg gtttactgat
     8401 gaccgtcacc gtcttctttg gcatttcggc gctgacagtt ctttcggtga ttgcggttcc
     8461 ggcgatcgcc tgtcttggcg gttattccgt gtggctggcc gttaacggca tgggcggcct
     8521 ggacgcctta aaagcggtcg ttcccgcaca accattagat ttcaatgtcg cgctggcgct
     8581 ggttgtgggg tcatttatca gtgcgggtac gctcaccgcc gactttgtcc gctttggtcg
     8641 caatgccaaa ctggcggtgc tggtggcgat ggtggccttt ttcctcggca actcgttgat
     8701 gtttattttc ggtgcagcgg gcgctgcggc actggggatg gcggatattt ctgatgtgat
     8761 gattgctcag ggcctgctgt tgcctgcgat tgtggtgctg gggctaaata tctggaccac
     8821 caacgataac gcgctctatg cgtcgggttt aggtttcgcc aacattaccg gtatgtcgag
     8881 caaaaccctt tcggtaatca acggtattat cggtacggtc tgcgcattat ggctgtataa
     8941 caattttgtc ggctggttga ccttcctttc ggcagctatt cctccggtag gtggcgtgat
     9001 cattgccgac tatctgatga accgtcgccg ctatgagcac tttgcgacca cgcgtatgat
     9061 gagtgtcaat tgggtggcga ttctggcggt cgccttgggg attgccgcag gccactggtt
     9121 accgggaatt gttccggtca acgcggtatt aggtggcgcg ctgagctatc tgatccttaa
     9181 cccgattttg aatcgtaaaa cgacagcagc aatgacgcat gtggaggcta acagtgtcga
     9241 ataacgcttt acaaacaatt attaacgccc ggttaccagg caaagagggg ctgtggcaga
     9301 ttcatctgca ggacggaaaa atcagcgcca ttgatgcgca atccggcgtg atgcccataa
     9361 ctgaaaacag cctggatgcc gaacaaggtt tagttatacc gccgtttgtg gagccacata
     9421 ttcacctgga caccacgcaa accgccggac aaccgaactg gaatcagtcc ggcacgctgt
     9481 ttgaaggcat tgaacgctgg gccgagcgca aagcgttatt aacccatgac gacgtgaaac
     9541 aacgcgcatg gcaaacgctg aaatggcaga ttgccaacgg cattcagcat gtgcgtaccc
     9601 atgtcgatgt ctccgacgca acgctgactg cgttgaaagc gatgctggaa gtgaaacagg
     9661 aagttgcgcc gtggattgat ctgcaaatcg tcgccttccc gcaggaaggg attttgtcgt
     9721 atcccaacgg tgaagcgttg ctggaagagg cgttacgctt aggggcagat gtagtggggg
     9781 cgattccgca ttttgaattt acccgtgaat acggcgtgga gtcgctgcat aaaaccttcg
     9841 ccctggcgca aaaatacgac cgtctcatcg acgttcactg tgatgagatc gatgacgagc
     9901 agtcgcgctt tgtcgaaacc gttgctgccc tggcgcaccg tgaaggcatg ggcgcgcgag
     9961 tcaccgccag ccacaccacg gcaatgcact cctataacgg ggcgtatacc tcacgcctgt
    10021 tccgcttgct gaaaatgtcc ggtattaact ttgtcgccaa cccgctggtc aatattcatc
    10081 tgcaaggacg tttcgatacg tatccaaaac gtcgcggcat cacgcgcgtt aaagagatgc
    10141 tggagtccgg cattaacgtc tgctttggtc acgatgatgt cttcgatccg tggtatccgc
    10201 tgggaacggc gaatatgctg caagtgctgc atatggggct gcatgtttgc cagttgatgg
    10261 gctacgggca gattaacgat ggcctgaatt taatcaccca ccacagcgca aggacgttga
    10321 atttgcagga ttacggcatt gccgccggaa acagcgccaa cctgattatc ctgccggctg
    10381 aaaatggatt tgatgcgctg cgccgtcagg ttccggtacg ttattcggta cgtggcggca
    10441 aggtgattgc cagcacacaa ccggcacaaa ccaccgtata tctggagcag ccggaagcca
    10501 tcgattacaa acggtgatcg gctgggctgt gtgttgacaa agcgagcgta gtttatgccg
    10561 gatgcggcgt gaacgcctta tccggcctac gtagagcacc gaactcgtag gcctgataag
    10621 cgtagcgcat caggcaattg tgtcagtggc taccgtgatt cattctcgcc aacatccgca
    10681 cattcctcca acgccatgtg caaaaatgcc ttcgcggcgg ctgtctgcca gctatttttc
    10741 cgccgcaaca aaaccgccgt tctctccagt agtggcgggg caagagaaat agctttaagc
    10801 ccgtcatgtt gtgtggcaat cgctgctggt aacaatgtgg aaagggaagt gcggcgaatc
    10861 agctccagaa ccgcgctaat tgagttcgcc tcaatgacca cctgtggatg cagccccgct
    10921 ttctcgcagt agtggtcaat ttgctctctg gtggcaaatt ccgcgctgag caggaccagt
    10981 ttttcatcat gcaagcgact caacgccacc tgttcacagg cagccagcgg atgatgttgc
    11041 gccacgacta acgctaaact ttctgtcagt aaaggaattg cctccagctc cggcgaatgc
    11101 acaggcgcga aggcaatccc aacgtccaac tcgtcgcggc aaagcatatc ctcgattttc
    11161 tcctgcgaca tttcctgtag ctggagcgtg atgccgggat agcgcgcata gaaatccgcc
    11221 attaaggggc cgataaagta gctcgtaaag gtgggggtga cggcgatacg cagcgatcct
    11281 cgcgtcagat cggcaacatc atgaatcgcc cgtttacccg cccccagttc ctgtaacgcc
    11341 cggctggcgt actgtcgcca gacttctcct gcatcagtga gatgaatcgt tcgcccgcta
    11401 cggtcaaaca gcggcacgcc taaactctcc tctaactggc gaatctgctg ggaaagcgca
    11461 ggctgagaga cgtgcaacgc actggcggca cgggtgaagc tgccatgttc agccacggca
    11521 agaaaataat tgatatgtcg agagagcatt cgccaaccta taagtaaatc caatagaact
    11581 catcataaat gagactttta ccttatgaca atcggcgagt agtctgcctc tcattccaga
    11641 gacagacaga ggttaacggt gaaagagatt attgatggat tccttaaatt ccagcgcgag
    11701 gcatttccga agcgggaagc cttatttaaa cagctggcga cacagcaaag ccctcgcacc
    11761 ctttttatct cctgctccga cagccgtctg gtccctgagc tggtgacgca acgtgagcct
    11821 ggcgatctgt tcgttattcg caacgcaggt aatattgtcc cttcctacgg gccggaaccc
    11881 ggtggcgttt ctgcttcggt ggagtatgcc gtcgctgcgc ttcgggtatc tgacattgtg
    11941 atttgcggtc attccaactg tggcgcgatg accgccattg ccagctgtca gtgcatggac
    12001 catatgcctg ccgtctccca ctggctgcgt tatgccgatt cagcccgcgt cgttaatgag
    12061 gcgcgcccgc attccgattt accgtcaaaa gctgcggcga tggtacgtga aaacgtcatt
    12121 gctcagttgg ctaatttgca aactcatcca tcggtgcgcc tggcgctcga agaggggcgg
    12181 atcgccctgc acggctgggt ctacgacatt gaaagcggca gcatcgcagc gtttgacggc
    12241 gcaacccgcc agtttgtgcc actggccgct aatcctcgcg tttgtgccat accgctacgc
    12301 caaccgaccg cagcgtaacc ttatttttaa accatcagga gatccaccat gattcagtca
    12361 caaattaacc gcaatattcg tcttgatctt gccgatgcca ttttgctcag caaagctaaa
    12421 aaagatctct catttgccga gattgccgac ggcaccggtc tggcagaagc ctttgtaacc
    12481 gctgctttgc tgggtcagca ggcgcttcct gccgacgccg cccgcctggt cggggcgaag
    12541 ctggatcttg acgaagacgc cattctactg ttgcagatga ttccactgcg tggctgcatt
    12601 gatgaccgta ttccaaccga cccaacgatg tatcgtttct atgaaatgtt gcaggtgtac
    12661 ggcacaaccc tgaaagcgtt ggttcatgag aagtttggcg atggcattat cagcgcgatt
    12721 aacttcaaac tcgacgttaa gaaagtggcg gacccggaag gtggcgaacg tgcggtcatc
    12781 accttagatg gtaaatatct gccgaccaaa ccgttctgac agccatgcgt aaccatcaaa
    12841 agacgttcac gatgctgctg gttctggtgc tgattggtct taatatgcga ccactgctca
    12901 cctccgtcgg gccactgcta ccgcaattgc gccaggcaag cggaatgagc tttagcgtgg
    12961 ctgccctgtt gaccgctctg ccggtggtta ccatgggcgg gctggcgctg gccggaagct
    13021 ggcttcatca gcatgtcagc gaacgtcgca gcgtcgccat cagtttgtta ctgattgccg
    13081 tcggtgcatt gatgcgtgag ctttaccccc aaagtgcgct gctgcttagc agcgcactgc
    13141 tcggtggggt ggggatcggc atcattcagg cagtgatgcc ttcggtgatt aaacggcggt
    13201 ttcagcagcg cacgccactg gtgatggggc tgtggtccgc ggctctgatg ggcggcggtg
    13261 gacttggtgc cgccctgacg ccctggttag ttcaacatag cgaaacctgg tatcaaacac
    13321 tcgcctggtg ggcgctgcct gccgttgttg cgctctttgc ctggtggtgg caaagcaccc
    13381 gcgaggtcgc ctcttcccac aagacaacaa ccactccggt tcgcgtggtt ttcactcctc
    13441 gcgcgtggac gctgggtgtt tacttcggtc tgattaacgg tggttacgcc agcctgattg
    13501 cctggttacc cgccttctat attgagattg gtgccagcgc gcagtacagc ggttccttgc
    13561 tggcattgat gacgcttggg caagccgcag gagctttgct gatgcctgct atggctcgcc
    13621 atcaggatcg gcgcaaactt ttgctgttgg cactggcgtt acaactggtg ggtttctgcg
    13681 gctttatctg gctgccgctg caattgccgg tattgtgggc gatggtgtgt gggttaggtc
    13741 tgggcggcgc gtttccgctc tgtttgctgc tggcgctcga tcactctgcg caaccggcta
    13801 ttgctggcaa gctggtggcg tttatgcagg ggatcggttt tatcatcgcc gggcttgccc
    13861 cgtggttttc tggtgtgctg cgtagtatca gcggcaatta cctgatggac tgggcatttc
    13921 atgcgctgtg cgtcgttggg ctgatgatta taaccctgcg ttttgcacca gcacgttttc
    13981 cgcagctgtg ggtcaaagag gcatgatgcg acgcttgttt ctgcgctttg ttcacgccgg
    14041 atgcggccta cgtaggtcac tgaatttgta ggcctgataa gcgaagcgta tcaggcaatt
    14101 tttataattt aaactgacga ttcaactttg taatctctga aataatattg cttatcccgg
    14161 tcgtttattt cgcgaataac ccggcaagga acgccagcag ccacgacgtt tggtggaatg
    14221 tcttttatga cgacactacc cgcgccaata acagaattat ccccgatggt gacgccagga
    14281 ttaatgacca catgacttcc gatccagacg ttattgccaa tcgttatcgg aaaagagtac
    14341 atctcaccgt tttttctcaa ttcatggtgt acagggtgtc ccgtaacgga aagagtaaca
    14401 ttgggtgcaa tcaatacgtt atcaccgatc gttaccgtgt agtcgtctac aatggttaaa
    14461 ttgaaatttg cataaaaatt gcggcctata tggatgttgg aaccgtaaga gaaatagacg
    14521 ggtggttcta cccaggcgtt ttcccctacc gtggcaaaca tttctttaat caggctttct
    14581 cttttttcaa cttctgatgg atgcgagtga ttaaactcat acattaacgt tttcccacga
    14641 agtctttttt ccggtaagcc ttcgcacata tcggtaaata gcttgcctgc ttttattctt
    14701 tcggtcattg gcatgttcat tgcgatcatt ccgttatgat atgttggtcg gataaggcgc
    14761 tcgcgccgca tccgacatta attgcttaag cgacttcatt cacctgacga cgcagcaggg
    14821 aaagcgggcc ggggccgcta agcgtgaaca cggaaattaa ggtgaagccc agcgccacca
    14881 gacccagcac cagataagcg ccctggaaac cgatgctttc atacatattg cccgccagta
    14941 cagacataaa aatcatcgcc agttgcttaa agaagcagaa gcagaccaga taaatcgtcg
    15001 ctgaaaaacg cacttcaaac tggctggtaa tatatttaaa gcagcccacc agcaggaacg
    15061 gtacttcaaa catatgcagc gttttcagaa taaccacttc cagcgctgag gtggcgaacg
    15121 atgagccaat aatacgtaca gacataatag tgccagccag cagcagggcg tttttcccac
    15181 cgatgcgatt aatgatcagt ggcgcaaaga acataatcga ggcgttaagt aattcgccca
    15241 ttgtcgttac gtagccaaat acccgcgtcc cctgttcacc ggtagcaaag aacgaagtaa
    15301 agaaattagc aaactgttgg tcaaaaacat cgtaggtgca ggaaacgcca ataacataca
    15361 gtgacaaaaa ccacagtttt ggctgtctga acagttccag cgccagctta aggctaaatg
    15421 ccgaatggtt ggcacctacc gcattggcaa ccgtggcgga agagggcgca tccgttttgg
    15481 cgaaaaagag taaaacggcg aggatgagtg cacagccaga acccagccag aaaacaaact
    15541 gattattgat ggtgaacatg atgccgacaa tcgaggcaca cagcgcccag ccaacacagc
    15601 caaacatccg cgcgcgacca aattcgaaat tactgcgacg gctgactttc tcgataaatg
    15661 cctctactgc tggcgcaccg gcgttaaaac aaaagcctag ataaatacca ccaacaatcg
    15721 atcctactaa aatgttgtat tgtaacagtg gcccgaagat aaaaataaag aacggcgcaa
    15781 acatcactaa catgccggta ataatccaca gcaggtattt gcgcagcccg agtttgtcag
    15841 aaagcagacc aaacagcggt tggaataata gcgagaacag agaaatagcg gcaaaaataa
    15901 tacccgtatc acttttgctg atatggttga tgtcatgtag ccaaatcggg aaaaacggga
    15961 agtaggctcc catgataaaa aagtaaaaga aaaagaataa accgaacatc caaaagtttg
    16021 tgttttttaa atagtacata atggatttcc ttacgcgaaa tacgggcaga catggcctgc
    16081 ccggttatta ttatttttga caccagagca actggtaatg gtagcgaccg gcgctaagct
    16141 ggaattccgc cgacactgac gggctccagg agtcgtcgcc accaatcccc atatggaagc
    16201 cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag atggcgatgg ctggtttcca
    16261 tcagttgttg ttggctgtag cggctgatgt tgaactggaa gtcgccgcgc cactggtgtg
    16321 ggccataatt caattcgcgc gtcccgcagc gcagaccgtt ttcgctcggg aagacgtacg
    16381 gggtatacat gtctgacaat ggcagatccc agcggtcaaa acaggccgca gtaaggcggt
    16441 cgggatagtt ttcttgcggc cctaatccga gccagtttac ccgctctgct acctgcgcca
    16501 gctggcaggt caggccaatc cgcgccggat gcggtgtatc gctcgctact tcaacatcaa
    16561 cggtaatcgc catttgacca ctaccatcaa tccagtaggt tttccggctg ataaataagg
    16621 ttttcccctg atgctgccag gcgtgagcgg tcgtaatcag caccgcgtcg gcaagtgtat
    16681 ctgccgtgca ctgcaacaac gccgcttcgg cctggtaatg gcccgccgcc ttccagcgtt
    16741 cgacccaggc gttagggtca atacgggtcg cttcacttac gccaatgtcg ttatccagcg
    16801 gtgcgcgggt gaactgatcg cgcagcgggg tcagcagttg tttttcatcg ccaatccaca
    16861 tctgtgaaag aaagcctgac tggcggttaa attgccaacg cttattatcc agctcgatgc
    16921 aaaaatccgt ttcgctggtg gtcagttgcg ggatggcgtg gggcgcggag gggagtgtca
    16981 cgctgaggtt ttccgccaga cgccactgct gccaggtgct gatgtgtccg gcttctgacc
    17041 atgcggttgc gttcggttgc actacgtgaa ccgttagcca gagttgtccg gcgctctccg
    17101 gctgcggtag ttcaggcagt tcaatcacct gtttaccttg tggagcgaca tccagaggca
    17161 cttcaccact cgccagcggc ttaccatcca gcgccaccgt ccagtgcagg agctcgttat
    17221 cgctatgacg gaacaggtat tcgctggtca cttcgatggt ttgcccggat aaacggaact
    17281 ggaaaaactg ctgctggtgt tttgcttccg tcagcgccgg atgcggcgtg cggtcggcaa
    17341 agaccagacc gttcatacag aactggcgat cgttcggcgt atcgccaaaa tcaccgccgt
    17401 aagccgacca cgggttgccg ttttcatcat atttaatcag cgactgatcc acccagtccc
    17461 agacgaagcc gccctgtaaa cgggggtact gacgaaacgc ctgccagtat ttagcgaagc
    17521 cgccaagact gttacccatc gcgtgggcgt attcgcaaag gatcagcggg cgcagttctc
    17581 caggcagcga aagccatttt ttgatggacc atttcggcac cgccgggaag agctggtctt
    17641 catccacgcg cgcgtacatc gggcaaataa tatcggttgc ggaggtgtcg gctccgccgc
    17701 cttcatactg taccgggcgg gaaggatcga cagatttaat ccagcgatag agtgcgtcgt
    17761 gattagcgcc gtggcctgac tcattcccca gcgaccagat gatcacactc gggtgattac
    17821 gatcgcgctg caccattcgt gttacgcgtt cgctcatcgc cggtaaccag cgcggatcat
    17881 cggtcagacg attcattggc accatgccgt gggtttcaat attggcttca tccaccacat
    17941 acaggccgta gcggtcgcac agcgtgtacc acagcggatg gttcggataa tgcgaacagc
    18001 gcacggcgtt aaagttgttc tgcttcatca gcaggatatc ctgcaccatc gtctgctcat
    18061 ccatgacctg accatgcaga ggatgatgct cgtgacggtt aacgccgcga atcagcaacg
    18121 gcttgccgtt cagcagcagc agaccatttt caatccgtac ctcgcggaaa ccgacatcgc
    18181 aggcttctgc ttcaatcagc gtgccgtcgg cggtgtgcag ttcaaccacc gcacgataga
    18241 tattcgggat ttcggcgctc cacagtttcg ggttttcgac gttcagacct agtgtgacgc
    18301 gatcggcata accgccacgc tcatcgataa tttcaccgcc gaaaggcacg gtgccgctgg
    18361 cgacctgcgt ttcaccctgc cacaaagaaa ccgtcacccg cagctcatcg cgcagctcgc
    18421 cgtacatctg aacttctgcc tccagtaccg cgcggctaaa atcatcatta aagcgagtgg
    18481 caacatggaa atcgctgatt tgtgtggtcg gtttgtgcag caacgagacg tcacggaaaa
    18541 tgccgctcat ccgccacata tcctgatctt ccagataact gccgtcactc cagcgcagca
    18601 ccatcaccgc gaggcggttt tctccggcgc gtaaaaatgc gctcaggtca aattcagacg
    18661 gcaaacggct gtcctggccg taaccgaccc agcgcccgtt gcaccacaga tgaaacgccg
    18721 agttaacgcc atcaaaaata attcgcgtct ggccttcctg tagccagctt tcatcaacat
    18781 taaatgtgag cgagtaacaa cctgtcggat tttctgcggg aacaaacggc ggattgaccg
    18841 caataggata ggtcacgttg gtgtagatgg gcgcgtcgta accgtgcatc tgccagtttg
    18901 aggggacgac gacagtatcg gcgtcaggaa gatcgcactc cagccagctt tccggcaccg
    18961 cttctggtgc cggaaaccag acaaattgcc actcaccatt caggctgcgc aactgttggg
    19021 aagggcgatt ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    19081 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aatacgacgg
    19141 ccagtgaatc cgtaatcata gtcatagctg tatcctgtgt gaaattgtta ttcgctcaca
    19201 atttcacaca acatacgagc cggacgcata aagtgtaaag cctggggtgc ctaatgagtg
    19261 agctgactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacttgtcg
    19321 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tcttgggcgc
    19381 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
    19441 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
    19501 tttgatggtg gttaacggcg ggatataaca cgagctgtct tcggtatcgt cgtatcccac
    19561 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag
    19621 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
    19681 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
    19741 aatttgattg cgtgtgagat atttatgcca gcccgccaga cgcagacgtg ccgagacaga
    19801 acttaatgga cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
    19861 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggag tctggtcaga
    19921 gacatcaaga aataacgccg gaacattagc gcaggctgct tccacagcag tggcatcctg
    19981 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac
    20041 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc
    20101 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gatggcgcgt gcagggccag
    20161 actggaggtg gcaacgccaa tcagcaacga ctgtttcccc gccagttgtt gtgccacgcg
    20221 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
    20281 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
    20341 tgcgacatcg tatagcgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
    20401 gcgctatcat gccataccgc gaaaggtttt gcgccattcg gcggggctga cgccgaagtg
    20461 agatttaaaa tgctgacgcc aggtcacagg tgagagaaaa cccacctcag ccgctatccg
    20521 ctcaacgggc aactgactgg atcccaacag ttcctggcta cggcgtaagc gttcagtaat
    20581 gagccattcg gcgatactcg aaccggtcgc tttcataaaa tggcgagtta atgtgcggcg
    20641 actcatcatt actcgctgcg ccagcgagtc gagatcatgc tgttgcgcaa tgttttgccg
    20701 caggtaatcc agcaggagat taatgcgttc atcatgggta tttttcggca ctggttgctc
    20761 aataaactgc gcctgaccgc cagtgcgata tggcggtacg accatccgtc gggcaatatg
    20821 gttagcatag tcgctgccaa aatgttgccg aacaatatac aaacagcaat ccagcgccgc
    20881 ggcagtaccg gcagaggtaa taatgccgtc atcgtctacg tacaacgcgt taatatccag
    20941 atgtgtttgt ggaaaacgtg cctgaaattc acgctcgaac tcccagtgtg tggcggcacg
    21001 cttatttttc agtaaacctg catagccgag cacaaacgtg cccaggcaaa gcccgacaat
    21061 ctgtgcgccg ttatcccgtg cggtcgttaa ggcttccagc agtttttgtg gcggtttttg
    21121 tgtaattgtt ccccagtaag gaattatcac aatatccgcc tggatgactg cggtatagtc
    21181 atgggtggcg gttacggaaa acccatcggc agagtccacg tttcccggct tttcagcgca
    21241 gataattacg tgaaagtgtt ttttctccgc cattttttcg ctaaatacga taaacggtac
    21301 ggaaaggtga aaaggactaa aaccggcaac ggcgataatg gcaacagtta gcgggcgcat
    21361 aacactcttt atctccgaat tagaatgtca acgtttcacc atcttctggt acgtttacaa
    21421 attcgataat ttgattatct ctggcgtatt ccttgagcgc atcacgcgtc agcaaacagt
    21481 ggtttacggc ctccatatgt gtcgcgacaa tttgcgcttc tggcagggta aaatgtgttt
    21541 tgagaatatc ttctgctccc ataattatcg cgccatgccc cagaacatgc gcaaatccgg
    21601 cattcaacac aaccacttct ggttggaaag ttagcattgt agcctcgacg tcgtcacgcc
    21661 agagggtatc gccagccaga tagagcgttt tctctgtcgg gtgctggaaa acaacgccgc
    21721 aggctgcgcc taaacgcttc gccatagccg ggtcggcata ggtgcgatcg gagccgtgct
    21781 gccctgtcgt tttatgcaga gtaatatcgc caaatttagt ctcgtcggta agtacggtaa
    21841 gatgcgtaaa tccctgttta ttcagtgatt cagcatcttg ctcattttgc acataaatca
    21901 gtttatcttt agggacgacg cgggaggctg tttcgtccca gtgatcttca tgcaaatggg
    21961 tcacgattag ggcgtcaacg ttgagtaacg ttgttaaatc gcacggtaat tctaccgttg
    22021 gattgcggat ttctgcgcgt gccgttccgg caaaacccgg ataacttcct ttaggcgcga
    22081 gtaacgggtc gataagaaat gttttgccag cgtattggat aatttgggtg gcattacgaa
    22141 tttgcgtaat tttcatgatt tgccccacat cacagtattg atgtcattag cgtagcgtgc
    22201 agcccgtact gtgcatgtgg gcaaatgggc gaataacgat gagaatgggc caatttacac
    22261 attggcgtaa ctagcgtaaa tgcacgccgc ttcgccgtcc ggccaccaga atagcctgcg
    22321 attcaacccc ttcttcgatc tgttttgcta cccgttgtag tgccggaaga tgcttttccg
    22381 ctgcttgttc gatggtcatt gcgctcgcca tatacaccag attcagacag ccaatcaccc
    22441 gctgttcact gcgcagcggt acggcgatag aggcgatctt ttcctcctga tcccagccgc
    22501 ggtagttctg tccgtaaccc tctttgcgcg cgcgcgccag aatggcttcc agctttaacg
    22561 gttcccgtgc cagttgatag tcatcaccgg ggcgggaggc taacatttcg attaattcct
    22621 tgcgttcttg ttcccagcaa aaggccagcc aggtcaggcc cgaggcggtt ttcagaagcg
    22681 gtaaacgtcg cccgaccatt gcccggtgaa aagataatcg gctgaaacga tgggtggttt
    22741 cgcgtaccac cattgcatca acatccagcg tggacacatc tgtcggccat accacttcgc
    22801 gcaacagatc gcccagcagt ggggccgcca gtgcagaaat ccactgttcg tcacgaaatc
    22861 cttcgcttaa ttgccgcact ttgatggtca gacgaaaact atcatcggag ggactacgcc
    22921 ggacatatcc ctcttcctgc agcgtctcca gcagtcgccg cacagtggtg cgatgcaggc
    22981 cgctgagttc cgccagcagc ccgacgctgg caccgccgtc aagtttattt aacatattta
    23041 ataacattag accgcgggtt aagccgcgca cggttttgta ttccgtctgc tcattgttct
    23101 gcatattaat tgacatttct atagttaaaa caacgttgtg cacctggtgc acattcgggc
    23161 atgttttgat tgtagccgaa aacacccttc ctatactgag cgcacaataa aaaatcattt
    23221 acatgttttt aacaaaataa gttgcgctgt actgtgcgcg caacggcatt ttgtccgagt
    23281 cgtgaggtac tgaaatggca atacaacacc ctgacatcca gcctgctgtt aaccatagcg
    23341 ttcaggtggc gatcgctggt gccggtccgg tcgggctgat gatggcgaac tatctcggcc
    23401 agatgggcat tgacgtgctg gtggtggaga aactcgataa gttgatcgac tacccgcgtg
    23461 cgattggtat tgatgacgag gcgctgcgca ccatgcagtc ggtcggcctg gtcgataatg
    23521 ttctgccgca cactacgccg tggcacgcga tgcgttttct caccccgaag ggccgctgtt
    23581 ttgctgatat tcagccaatg accgatgaat ttggctggcc gcgccgtaac gcctttattc
    23641 agccgcaggt cgatgcggtg atgctggaag gggtgtcgcg ttttccgaat gtgcgctgct
    23701 tgttttcccg cgagctggag gccttcagtc agcaagatga cgaagtgacc ttgcacctga
    23761 aaacggcaga agggcagcgg gaaacggtca aagcccagtg gctggtagcc tgtgacggtg
    23821 gagcaagttt tgtccgtcgc accctgaatg tgccgtttga aggtaaaact gcgccaaatc
    23881 agtggattgt ggtagatatc gccaacgatc cgttaagtac gccgcatatc tatttgtgtt
    23941 gcgatccggt gcgcccgtat gtttctgccg cgctacctca tgcggtacgt cgatttgaat
    24001 ttatggtgat gccaggagaa accgaagaac agctgcgtga gccgcaaaat atgcgcaagc
    24061 tgttaagcaa agtgctgcct aatccggaca atgttgaatt gattcgccag cgtgtctaca
    24121 cccacaacgc gcgactggcg caacgtttcc gtattgatcg cgtactgctg gcgggcgatg
    24181 ccgcgcacat catgccggta tggcaggggc agggctataa cagtggcatg cgcgacgcct
    24241 ttaacctcgc atggaaactg gcgttggtta tccaggggaa agcccgcgat gcgctgctcg
    24301 atacctatca acaagaacgt cgcgatcacg ccaaagcgat gattgacctg tccgtgacgg
    24361 cgggcaacgt gctggctccg ccgaaacgct ggcagggtac gttacgtgac ggcgtttcct
    24421 ggctgctgaa ttatctgccg ccagtaaaac gctacttcct cgaaatgcgc ttcaagccga
    24481 tgccgcaata ttacggcggt gcgctggtac gtgagggcga agcgaagcac tctccggtcg
    24541 gcaagatgtt tattcagccg aaagtcacgc tggaaaacgg cgacgtgacg ctgctcgata
    24601 acgcgatcgg cgcgaacttc gcggtaattg gctggggatg caatccactg tgggggatga
    24661 gcgacgagca aatccagcag tggcgcgcgt tgggcacacg cttcattcag gtggtgccgg
    24721 aagtgcaaat tcataccgca caggataacc atgacggcgt actacgcgtg ggggatacgc
    24781 aaggtcgcct gcgtagctgg ttcgcgcaac acaatgcttc gctggtggtg atgcgcccgg
    24841 atcgctttgt tgccgccacc gccattccgc aaaccctggg caagaccctg aataaactgg
    24901 cgtcggtgat gacgctgacc cgccctgatg ccgacgtttc tgtcgaaaag gtagcctgat
    24961 atgcacgctt atcttcattg tctttcccac tcgccgctgg tggggtatgt cgacccggca
    25021 caagaggtac tcgatgaggt caatggcgtg attgccagcg cccgcgagcg tattgcggct
    25081 ttctcccctg aactggtggt gctgtttgcg ccagatcact acaacggctt tttctatgac
    25141 gtgatgccac cgttctgttt aggcgttgga gcgacggcaa ttggtgattt cggcagtgcg
    25201 gcaggagagc tgcccgtgcc tgtggagctg gcggaggcct gtgcgcatgc cgtcatgaag
    25261 agcgggatcg atcttgccgt ttcttactgt atgcaggtgg accacgggtt cgcccagccg
    25321 ctggagttcc tgctcggtgg gctggataag gtgccagttc tgcctgtgtt catcaacggt
    25381 gtcgccacgc cgctgcccgg tttccagcgt acccgcatgt tgggtgaagc cattggacgt
    25441 ttcaccagca ctctcaataa acgcgtgctg ttcctgggtt ccggtgggct ttcccatcag
    25501 ccgccggtgc ctgaactggc gaaagccgat gcccatatgc gcgaccgtct gttggggagc
    25561 gggaaagatt tacccgccag tgagcgcgaa ttgcgtcagc aacgggtgat tagcgccgct
    25621 gagaagtttg ttgaggatca gagaactctg catccgctca acccgatttg ggataaccag
    25681 ttcatgactt tgctggagca gggacgcata caggaactgg atgccgtcag taacgaagag
    25741 ctttccgcca ttgccggaaa gtcgacacat gaaatcaaaa cctgggtcgc cgcttttgcc
    25801 gctatttctg catttggcaa ctggcgtagc gaagggcgtt attaccgccc aatcccggag
    25861 tggattgccg gatttggctc gttaagcgcc agaacagaga actgaatttg caggagaaga
    25921 tgatgagtta tcagccacaa accgaagccg ccaccagccg ttttctgaat gtagaagaag
    25981 cgggtaaaac gctgcgcatc cattttaatg actgcggaca aggcgacgaa accgttgtcc
    26041 tgctgcatgg ttccggcccg ggtgctactg gctgggcgaa cttcagccgc aatatcgatc
    26101 cgctggtaga ggcgggctat cgggtgatcc tactggattg tccgggttgg ggcaagagcg
    26161 attcgatcgt taatagtggt tcacgatcgg atcttaatgc gcgcatcctg aaaagtgtgg
    26221 tggatcaact ggatatcgcc aaaatccacc tgttgggcaa ctcgatgggc ggccatagtt
    26281 ctgtggcgtt cacccttaac tggccggagc gcgtcggcaa actggtgctg atgggcggcg
    26341 gtacgggcgg catgagtttg tttacgccga tgccaaccga aggtattaag cgactgaatc
    26401 agctttatcg tcagccgact atcgaaaacc tgaaactgat gatggatatc ttcgtttttg
    26461 ataccagcga tttgaccgac gccctgtttg aagcgcgcct gaataatatg ctgtcgcgcc
    26521 gcgatcacct ggaaaacttc gttaagagcc tggaagccaa tccgaaacag ttccctgatt
    26581 ttggcccacg tctggcggaa atcaaagcgc aaaccctgat tgtctggggg cgcaacgacc
    26641 gctttgtgcc gatggatgcg gggctgcgtc tactgtccgg cattgctggt tctgaactgc
    26701 atatcttccg cgactgtggt cactgggcgc agtgggaaca tgccgatgct ttcaatcaac
    26761 tggtgctgaa tttcctcgca cgtgcttaag gaacggtcat gacgaagcat actcttgagc
    26821 aactggcggc ggatttacgc cgcgccgcag agcagggcga agcgattgcg ccgctgcgcg
    26881 atctgattgg tatcgataac gctgaagcgg cctacgccat tcagcacata aatgtgcaat
    26941 atgacgttgc gcaggggcgt cgcgtggtgg ggcgtaaagt gggcctgaca catccgaaag
    27001 tgcagcaaca actgggcgtt gatcaaccgg atttcgggac tttatttgcc gacatgtgtt
    27061 atggcgataa cgaaaccatt cctttttccc gtgttctgca accccgcatt gaagcggaga
    27121 tcgcactggt gttgaaccgc gatttacccg caactgatat caccttcgac gaattgtata
    27181 acgccattga atgggtactt ccggcgctgg aggtggtggg gagccgcatt cgcgactggt
    27241 cgattcagtt tgtcgatacc gtggcagata atgcctcctg cggggtgtat gtcatcggcg
    27301 gtccggcgca acgtccggcg gggttagacc tgaaaaactg cgccatgaag atgacgcgta
    27361 ataacgaaga ggtttccagc gggtgcggca gcgaatgcct gggacatccg cttaatgccg
    27421 ccgtctggct ggcacgcaaa atggccagtc tgggtgaacc gctgcgcgcc ggagatatca
    27481 ttcttaccgg ggcattaggt ccgatggtgg cagtgaatgc gggcgatcgt tttgaagccc
    27541 atattgaagg cataggttca gttgctgcga cattttcaag cgcagcccca aaaggaagtc
    27601 tgtcatgagt aagcgtaaag tcgccattat cggttctggc aacattggca ccgatctgat
    27661 gattaaaatt ttgcgtcacg gtcagcatct ggagatggcg gtgatggtcg gcattgatcc
    27721 tcagtccgac ggtctggcgc gtgccagacg tatgggcgtc gccaccaccc atgaaggggt
    27781 gatcgggctg atgaacatgc ctgaatttgc tgatatcgac attgtatttg atgcgaccag
    27841 cgcgggcgcg catgtgaaaa acgatgccgc tttgcgcgaa gcgaaaccgg atattcgctt
    27901 aattgacctg acgcctgctg ccatcggtcc ttactgcgtg ccggtggtta acctcgagac
    27961 gaacgtcgat caactgaacg tcaacatggt cacctgcggc ggccaggcca ccattccaat
    28021 ggtggcggca gtttcacgcg tggcgcgtgt tcattacgcc gaaattatcg cttctatcgc
    28081 cagtaaatct gccggacctg gcacgcgtgc caatatcgat gaatttacgg aaaccacttc
    28141 ccgagccatt gaagtggtgg gcggcgcggc aaaagggaag gcgattattg tgcttaaccc
    28201 agcagagcca ccgttgatga tgcgtgacac ggtgtatgta ttgagcgacg aagcttcaca
    28261 agatgatatc gaagcctcaa tcaatgaaat ggctgaggtg gtgcaggctt acgtaccggg
    28321 atatcgcctg aaacagtgcg tgcagtttga agttatcccg caggataaac cggttaattt
    28381 gccgggcgta gggcagttct ccggactgaa aacagcggtc tggctggaag tcgaaggcgc
    28441 agctcattat ctgcctgcct atgcgggcaa cctcgacatt atgacttcca gtgcgctggc
    28501 gacagcggaa aaaatggccc agtcactggc gcgcaaggca ggagaagcgg catgaacggt
    28561 aaaaaacttt atatctcgga cgtcacgttg cgtgacggta tgcacgccat tcgtcatcag
    28621 tattcgctgg aaaacgttcg ccagattgcc aaagcactgg acgatgcccg cgtggattcg
    28681 attgaagtgg cccacggcga cggtttgcag ggttccagct ttaactatgg tttcggcgca
    28741 catagcgacc ttgaatggat tgaagcggcg gcggatgttg tgaagcacgc caaaatcgcg
    28801 acgttgttgc tgccaggaat cggcactatt cacgatctga aaaatgcctg gcaggctggc
    28861 gcgcgggtgg ttcgtgtggc aacgcactgt accgaagctg atgtttccgc ccagcatatt
    28921 cagtatgccc gcgagctcgg aatggacacc gttggttttc tgatgatgag ccatatgacc
    28981 acgccggaga atctcgccaa gcaggcaaag ctgatggaag gctacggtgc gacctgtatt
    29041 tatgtggtgg attctggcgg tgcgatgaac atgagcgata tccgtgaccg tttccgcgcc
    29101 ctgaaagcag tgctgaaacc agaaacgcaa accggcatgc acgctcacca taacctgagt
    29161 cttggcgtgg cgaactctat cgcggcggtg gaagagggct gcgaccgaat cgacgccagc
    29221 ctcgcgggaa tgggcgcggg cgcaggtaac gcgccgctgg aagtatttat tgccgccgcg
    29281 gataaactgg gttggcagca tggggccgat ctctatgcgt taatggatgc cgctgacgac
    29341 ctggtgcgtc cgttgcagga tcgaccggta cgagtcgatc gcgaaacgct ggcgctggga
    29401 tacgctggtg tttactccag cttcctgcgt cactgtgaaa cggcggcggc gcgttatggc
    29461 ttaagcgcgg tggatattct cgttgagctg ggcaaacgcc ggatggttgg cggccaggag
    29521 gatatgatcg tcgacgtggc gctggatctg cgcaacaaca aataatattg actgccgaga
    29581 acgtgcattt tgtatgccca tcccagcacg actgccggat gcggcgtgaa cgccttatcc
    29641 ggcctacgga tggcgcgaga atttgtaggc ctgataagac gcgttagcgt cgcatcaggc
    29701 atctgcgcac gactgccgga tgcggcgtga acgccttgtc cggcctacgg gtggcgtgag
    29761 aatttgtagg cctgataaga cgcgcaagcg tcgcatcagg catctgcaca cgactgccgg
    29821 atgcggcgta aacgccttat ccggcctacg gatggcgcgg gaatttgtag gcctgataag
    29881 acgcgcaagc gtcgcatcag gcatctgcgc acgactgccg gatgcggtaa acgtcttgtc
    29941 cggcctacat ttcgcccgta ggcagtcatt aaatagttct gattacgcgg ctggcaatgt
    30001 atcagtcgcg attcacctgc actcgcaacg aggttcatct tatgtcgact cgtacccctt
    30061 catcatcttc atcccgcctg atgctgacca tcgggctttg ttttttggtc gctctgatgg
    30121 aagggctgga tcttcaggcg gctggcattg cggcgggtgg catcgcccag gctttcgcac
    30181 tcgataaaat gcaaatgggc tggatattca gcgccggaat actcggtttg ctacccggcg
    30241 cgttggttgg cggaatgctg gcggaccgtt atggtcgcaa gcgcattttg attggctcag
    30301 ttgcgctgtt tggtttgttc tcgctggcaa cggcgattgc ctgggatttc ccctcactgg
    30361 tctttgcgcg gctgatgacc ggtgtcgggc tgggggcggc gttgccgaat cttatcgccc
    30421 tgacgtctga agccgcgggt ccacgttttc gtgggacggc agtgagcctg atgtattgcg
    30481 gtgttcccat tggcgcggcg ctggcggcga cactgggttt cgcgggggca aatttagcat
    30541 ggcaaacggt gttttgggta ggtggtgtgg tgccgttgat tctggtgccg ctattaatgc
    30601 gctggctgcc ggagtcggcg gtattcgctg gcgaaaaaca ggctgcgcca ccactgcgtg
    30661 cgttatttgc gccagaaacg gcaaccgcga cgctgctgct gtggctgtgt tatttcttca
    30721 ctctgctggt ggtttacatg ttgatcaact ggctaccgct gcttttggtg gagcaaggat
    30781 tccagccatc gcaggcggca ggggtgatgt ttgcactgca aatgggtgcg gcgagcggga
    30841 cgttaatgtt gggcgcattg atggataagc tgcgtccagt aaccatgtcg ctactgattt
    30901 atagcggcat gttagcttcg ctgctggcgc ttggaacggt gtcgtcattt aacggtatgt
    30961 tgctggcggg atttgtcgcg gggttgtttg cgacaggtgg gcaaagcgtt ttgtatgccc
    31021 tggcaccgtt gttttacagt tcgcagatcc gcgcaacagg tgtgggaaca gccgtggcgg
    31081 tagggcgtct gggggctatg agcggtccgt tactggccgg gaaaatgctg gcattaggca
    31141 ctggcacggt cggcgtcatg gccgcttctg caccgggtat tcttgttgct gggttggcgg
    31201 tgtttatttt gatgagtcgg agatcaagaa tgcagccgtg tgccgatgcc tgacgcgcct
    31261 tattaagcag gggcgaaagg gaattgcccc caatacaccc acggcgttta caggtatact
    31321 cgctaaaaat tattcagcgg gtttggaaac atagatggca aaacttacct tacaagagca
    31381 gttgctcaaa gcaggattag tcaccagcaa aaaagcggcg aaggtcgaga gaacggcgaa
    31441 aaaatcgcga gttcaggcgc gtgaagctcg ggctgcggta gaagaaaata aaaaggcaca
    31501 gcttgagcgt gataaacagc ttagcgaaca gcaaaaacaa gcggcgttgg cgaaagaata
    31561 taaagctcag gtgaagcagc ttattgaaat gaaccggatc accattgcca atggcgatat
    31621 tggttttaac ttcactgacg gcaatctgat taagaagatt tttgtcgata agctcacaca
    31681 ggcgcagttg attaatggtc gtctggcgat tgcccgcttg ttggtcgata acaatagcga
    31741 aggtgaatac gcaattattc ccgccagcgt tgccgataaa attgctcagc gtgatgccag
    31801 cagtattgtg ttgcacagcg cgctcagtgc cgaagagcag gatgaagacg atccgtatgc
    31861 cgacttcaaa gtgcctgatg atttgatgtg gtaaacatag ataagtcgtg cgcagatgcc
    31921 tgatgcgacg ctatgcgcgt cttatcatgc ctacggctta cgggcgaaat gtaggcggat
    31981 aaggcgttct cgccgcatcc ggcagtcgag cactattatc aacgcatatt cagtttattg
    32041 gcgtggtagg caatatgctc gccaataaaa ctggagacaa aataatagct gtggtcgtaa
    32101 ccctcgtgat aacggattaa cgtcttgata ttcatctcct ggcagatctt ttcaagattt
    32161 ggagtccgca gctgttctgc gtaaaaatca tcactcaacc cctgatcaac catgatttcc
    32221 gcaacgcgtt gaccttgtga aataagactc accggatcgt aatccaacca ggcctcttta
    32281 ttttcaccaa gatatgcggc aaaggcttgc tgtccccacg gtacttgcga tggggagaca
    32341 atgggcgaaa acgccgagac gctgacatat tcatctgggt tacgtaacgc cagcaccagc
    32401 gcgcccagcc cgcccataga atgaccagag atagactttt tggtcgttgc cggaaaatga
    32461 tgcatcacta aatccggcag ttcgttgcgg atatagtcat acattttgta atgttcattc
    32521 cacggcgctt gcgtcgcgtt caggtaaaac ccggcacctt gcccgagatc gtaacggtca
    32581 gcatctgcga catgactgcc tcgcggactg gtgtccggcg caacaacaat aatgttgtgc
    32641 tcagccgcgt aacgctgcat ccccgattta gtaatgaaat tctgctcgtt gcaggtcagg
    32701 cctgaaagcc agtacagcac cggcaatttt tcattcgcgg cttttggtgg gagatagacg
    32761 ccgacattca tttcacattt cagtgattgg gaataatgcc gatacacatt ttgccagccg
    32821 ccaaagctgg catgtttttc aatgagttcc atcagtcatt atctcacggt taatcccccg
    32881 gacggctgcc cggggagagt attaagaacc ccgtctgaag tggacggggt aaaccagcgg
    32941 gaaatcagta acgaattacg gttcgaatgg atttaccttc atgcatcagg tcgaaggcgt
    33001 cattaatttc atccaggctc atggtatgcg tgacaaacgg ttccagatcg atatcacctt
    33061 tcatcgcatc ttcaaccata cccggtaact gggaacgacc tttcacgccg ccaaacgcgg
    33121 aacctttcca tacgcgacca gtgaccaact ggaatggacg ggtggagatt tcctgaccgg
    33181 cacccgcgac cccgatgatc accgactgac cccagccgcg gtgcgcactt tccagcgccg
    33241 cacgcatcac gttgacatta ccgatgcatt caaaggtatg gtcgataccc catttgttga
    33301 tatccagcag gacatcttta atcggtttgt cgtagtcatt cgggttaatg cagtcggtgg
    33361 caccgaagcg acgcgccaga tcgaatttct tcgggttggt atcgatagcg ataatccgtc
    33421 ccgctttcgc ctgacgcgcg ccctgaacca ctgccagacc aatcgcgcca agaccaaaca
    33481 cagcaacaga atcccctggc tggactttag ctgtgttgtg taccgcgcca ataccggtgg
    33541 tcacgccaca gcccagcagg cagacgtgtt catggtttgc ttctggatta attttggcca
    33601 gagacacttc cgcgactacg gtgtattcac tgaatgtaga gcaccccatg tagtgataaa
    33661 gcggctgccc gttgtaagaa aaacgggtgg tgccgtctgg catcaggcct ttaccctggg
    33721 tttcgcgaac cgcaacacag aggttagttt tgccagaacg acagaactca cactcgccgc
    33781 actctgcggt gtaaagcggg atcacatggt cgcctggttt gacgctggtt acgccttcac
    33841 cgacttcaac cacaacgcca gccccttcgt gaccgagaac caccgggaat acaccttccg
    33901 ggtcatcgcc ggagagggta aatgcgtcgg tatggcaaac gccggtatgg gtgactttaa
    33961 tcagcacttc accttttttc ggtggtgcaa cgtcaatttc aacgatttcc agcggtttac
    34021 cgggagcaaa tgcaacggca gcacgtgatt tcatctctcg ctcttcctca atatggtaat
    34081 agattcagct atttaagata ggcacgaacc agttcaatag tgtcgtcaac ggattggctg
    34141 acttcgcggc tgtagcagtc atttcggtca aacgtttccc ggatatggct ttcaagcact
    34201 tctgccatca gcccattagc cgcgccccga acggcagcga tctgttggag tatggcacgg
    34261 cattcggcat caccctccag cgaccgttcc agagcatcaa tctgccctcg aatacgacga
    34321 actcgagtaa ggaccttttt cttctcttcc ggagtactgg gcatttcgca cctcatcatc
    34381 tgcacgcaat atactatagg ggggtatgct atatgtcaat acataccccc ctatagtata
    34441 taacactgga gaataattgt tgtccgaagc ctaaccaggt gaacggtccc tcaagacaga
    34501 tggggaccgt ttatgtgcta tttttatgcc gcatccgcta aaacaaacat cccgtaagga
    34561 tggatttcga ggtaatagtg ctcaccaata tcgggctgta aacgggtggc gtttacttgt
    34621 aataaaatct cctgtccagg ccactctacg gtcacttcgt actgcggccc catgtaggcg
    34681 acatggcgaa taacacaacg ctgactctct tcgccgtgat tgcttaaggt gatggcttcc
    34741 gggcgaacgc cgacggtgcc gctaccttgc gccgggaaat gcgccgcgcg cggcaggcga
    34801 taaccgtaga tatcgacata ctcttcgcta aagttcgccg ggaacaggtt ggcgtcgccc
    34861 ataaaacttg ccataaatcg ggaggctggc tgacgataga gatcctgcgg tgagccaatc
    34921 tgcatgatgt gacctttatt catcaccagc acagtgtcgg acaccgcgaa agcttcactt
    34981 tgatcgtgag tgacgtagag cgacgtgata ttaaactgct tttgcagctc gcggatctta
    35041 tcgcgcatac tgcggcgcag gttggcatcg aggttactta acggctcatc aaacagcagc
    35101 acctttggct tgaggatcaa cgcgcgggcc agtgccacac gctgttgctg accaccggaa
    35161 atctgatcga catagcggtc ctcgaagcct tccagatcca ccattgccag cgcctccttc
    35221 acccgctgtt tcacttcgct acgcgacacg ccaagcatct tcagcccgta gccaacgttt
    35281 tcacccagcg acatatgcgg gaacagagcg tatgactgaa acaccatgca gatatcgcgc
    35341 tgttgaatgg aacgatgcgt gacatcttcg ccatcaataa aaatttgccc ttcgctcggt
    35401 ttttccagcc cggcaaccag acgcaaaatg gtggttttgc cacagccgga aggaccgagc
    35461 agcgtcacca tttgcccttg tggaatggtg agattgatat tgtcgataac cgtattactg
    35521 ccgaatcgtt tagtgacgtt gcgcagttca acaaaatttt tctgactcat agtgcgctcc
    35581 attacgcctg gtttttggct ttagaacggg agatacgagc ttctccgatc agccagtcaa
    35641 agaggaaaat aatcgccagc atcaccacga tcagaatcga accataggcg atggctacgc
    35701 catattcgcc gtcttcaacg cggttaagaa tgtaggcggt tgccacgcgg gtgtccggcg
    35761 tgacgagaaa gacgatggcg ctgaccgtcg tgatggcccg cacaaagctg tagatcaggg
    35821 ccgagagaat ggccgggcgc agcagcggca gtaaaatgtg cgtaatggtt cgcagtgaac
    35881 ccgcgcgcag gctgagtgag gcttcatcca gcgatttgtc gatctgcccc aggcctgcaa
    35941 tcccggcgcg aatacccacc ggaacgttac gcatcaccat cgagataatc acaattgccg
    36001 ccgttccggt gagataaacc ggagcgctgt taaaggcaag aatatacgaa acgcccgcca
    36061 ccgtacccgg tacggcaaag cacagcatgg tggtgaactc gatggtcttt ttgcctttga
    36121 attgttgacg caccaccacc caggcgatga gcaggccaaa gatagcggta atgggagcgg
    36181 caatcccggc gtagagcagg gtatccagca gtgaaggcca cgcgccgtcg ctcattccct
    36241 gaccaaatag cttgataaag ttatccagcg tcagggtgta gtccacgccc cagttcaccg
    36301 tgaagctgcc gtagaaaatg ctgccgtaga gcagggcgtt aaaggcaatc catatcgcaa
    36361 gtatggcgac cacgctccat accagggtga ccggtagcgg ctggacgtcg ccacggtagg
    36421 atttgccgga aaccgtgacg taagagcgtt taccaatcca catatactgg atgcagaaca
    36481 ccagcagcga gaacagcagc aggaaagcgc caagggtgct ggcggcctga taatccagct
    36541 gcgagccggt aatatagaag taaatttgcg tcgccagtac gtcgaagtta ccgccaagta
    36601 ccagcgggtt actgaaatca gccagtgact gtaccacgac gattaaaaag gcattggcga
    36661 gtgcgggctt caacaacggc acaaacacgc cgttaaaggt ttgccagcgg ctggcgcgca
    36721 gggtgtagga ggcctcttcc agcgagggat gaatggtttt aatggccccg tcgaggatca
    36781 taaatgccat tggcgtgaat gccagaacct gtgccagcca gatcccggta aaaccgtaca
    36841 gccagttggt gttggtcagc ccaaaccact ccaccataaa ttccgtaaca tagccggagc
    36901 gccccatcat gagcgtcacg cctaagccga ccacaaacgg tggcgtaacg atgggcaaaa
    36961 tggaaaagat gcgaccaata atggcgctgc gtttagcgat gcgcgtggta taaatcgcca
    37021 gcaccaggcc gaagaaagta cagcctatcc ccaccgctat cgacaagacg atagagttga
    37081 cgatcacctg aacaatatgt gcctgcgaga gcacggtcat aaacgccagc ggggcgaact
    37141 ctcccgcgtc gttggtgaac atcgggataa aaatggcgat gctcggccag acgataaaaa
    37201 tgccaatcag cgccacaatc gcaatcagtg agccgatcac gaagcggtcg ccacccagcc
    37261 attcgaggcg ggttaaggcc agcgtcataa tcgcgcctag ttcgacaaac agcacgatgg
    37321 cggcgtagcc taaaccgcgt ccaaccacgg tggcgctgat gacgataaac gccatacaga
    37381 gcaatgccca gccggcatcg aaataatgac ggctacgttg ttcgcgcgtg gcctcactaa
    37441 acgggcggac cagcaacagg ctcgggagta aataccacag ccagctgata ttaaaatgcg
    37501 accagccgta cgccccgagg atctcatcac tcgtggactc cagcaggcca taatcgaggc
    37561 tccagctcgg caacaggaca aatgccagcc aacccagcaa aacccaaagg aaaatcgcat
    37621 cccgcttttt cacgggatgg agtgcaagtg tctgtgacat agcagttccg gtatggttgg
    37681 ccggggcagg cgtctgcgct gcacccggaa aatacgccta atgcgggttt atttacccat
    37741 cttcacttcg ctgacccatt tattaatcaa tgccttacga acatccgtgg aaccatattt
    37801 atccatgtcg tagttgatta atttcaggtc gtcgagcttc agtgaatttg gcgacgtttc
    37861 ggcggtggta ttggtcagga tctgataaga cttccctttc ttccacgcca gttcctgagc
    37921 ttcttttgat aatacccagt ccacaaacag tttggcgttg tcgaggttgc gcgcgccttt
    37981 cagaatgctg acgccgccaa tttcgtagcc tgtaccttca cacggggaaa tcaactccag
    38041 cggcgcgccc tgttcttttt ccagcgagta gtcatgcagg aagccaatac caatcgccgt
    38101 ttcgccacgg gcggcgttac gtgccggggc tatgcctgat ttggtgtact gagagacgtt
    38161 ggcgttcagt tgttttagat aatcaaaggc ctgatcttcc ccccacagct gcgcaaaagt
    38221 agccagggcg gtgtaggcag taccggaact ttgcggatcg gcaatttgaa tttcgccttt
    38281 atattccggt ttggtgagat ctttccagca cttcggcacc ggcaggtttt tctctttcag
    38341 acgatcggtg tttacgccaa aaccgaggat ccccacataa acggcagagg acagattccc
    38401 tttcactttt gccggatcgc ggaatttctc catgatctgc tcaaggttag gggatttata
    38461 cgcctgtagc aggcccatct cgccagcctg cgattgcggg tctaaagtgc cgccgtacca
    38521 gacgtcagcc tgggggtttt tcttttcagc atcgactttt gccagggtgc ttccggaacc
    38581 gttgcggata aacgaggttt ttacatcata tttctcgcca aacgctttgg tttcggcctc
    38641 acacatttca ttggtggcgc tgcaatagac caccagccgg ccttttgcct gcgcggcacc
    38701 cgttaaagtc gccagcgcaa cggcagaagc aatcagagta gaggtaaagg tcattttcat
    38761 tgttttatcc ttcgagcgtg agtggtggtg atgcccgcca tcagtaacgg catcagcagt
    38821 aaacccataa gtactgcggc gaccgagagt aaactgaaca ttcccggcca gccgtagcgt
    38881 tcgatgacca gcgacagcgg ccagcctgcc agcgccgcgc caaggtaagc gaacaggccg
    38941 agaaatccgg tgattgaacc cgccgcagct ttgtgcccac actccaccgc cgcaagtcca
    39001 atcagcattt gtgggccaaa gacgaaaaag cccacggtaa aaaagcacac tgccagcaga
    39061 gcgtagtgat gaaccggtgc cagccacagt gcggcgactg acaccatcaa ccccaacgtg
    39121 aacagcaaaa tcattggcgc tcgttgcccg ctaaacagca aatccgatcc ccatccggca
    39181 aataacgcgc cgagtaagcc gcccacttca aacagcatta ccgtggcgtt ggcgctgagc
    39241 aggttgacac cgtggctttc cgtcagccag atattgcccc agtcgttaag cgcaatccgg
    39301 atcacataaa ccagtacata agagacgccc agtagccaga tcagcggatt ttgcagcatg
    39361 gtggtgcgca acatttgcca cagccccatc ggcggacttt gttgttcctg acgtaactcc
    39421 agcggatcgt gacgccagtg accaactgtc ggtaatccct cttcctgggg cgtgcctttg
    39481 agttgtagcg ttaaccagat ccctaatgcc atgctgatta tccccggtgt cagcatcgcg
    39541 gcctgccagc cccaccagtg ggcggcaaag gcgctaatca gcggaataat cgctccgcca
    39601 atgttgatcg acatattcca gcagccccac cagaaaccgc gctcattgcg tgagtaccag
    39661 tgggtcagca agcgggcaca cggtggccat ccccagccct gaaaaaaacc gttcagtgtc
    39721 cagaccacaa gcagaagcgt aagtgattcg ccaaaggcaa acaccacgtt caataacccg
    39781 gtggcgaaca agccgacgcc cataaaaccg cgttgtccat ggctgtcatg ccacagtccg
    39841 gcggtaaatt tcgacaatcc gtagctgaga taaaacagcg aacccagcag gccaatgtcg
    39901 cctttatcca gtccgaggtc ggtttgtagc gccggaagga cgtaattcac gctttttcgc
    39961 gtcagataaa atgcggcgta gccaataatc atgcacatca gcaatcgcgg acgtaatgtc
    40021 cggtagcatt gattaatttc tctcgctgaa cgcgcgtgca tggttgtctc catgttgctg
    40081 actatatgaa aatgaaagtg aaaggggatg agagaaaatc cgggatacct aagacttttt
    40141 cctggttatc cggcgatttg ttgcaaaaat gtgggcaagt taacaattac ccgcgtgccg
    40201 tgctgttttt cgagcgttaa gtcgccgccc agggcgctga cgcgctcacg catcccctga
    40261 atgccaaatc cggccatttt cccgctggcg atccccacac cgttgtcgct gacttccaga
    40321 tgcaatacct ctccctgctg gcgcaggata atcgtcactt cgctggcttc ggcgtgttta
    40381 cagacgttat tgagcaactc ctgtaataac cgatacaagg taaagcgcac cgtttcgttt
    40441 tctggtgtgg agttcagttg ataggcaaac tggcaatgaa tgccgcgttc actaaaggca
    40501 aattcattaa tcagatgcaa caacgcttcg cgaaatgtca gttcatcaag cgcgggaggc
    40561 cgcaactggc gcagcaattg gcgggtggag agatgaatgc ggcgggcgag ctcattgatt
    40621 tggctggctg cggattgaat ttgcgccgga tcacgcgccc gcttcaccag ttgcgactgg
    40681 atctgaatgg cggtgatgtt ctgaccgatt tcgtcgtgca gctctcgcgc caggtttttt
    40741 cgcgtgtcct cctcggtgtg gatcagcttt tccgccaatg cccgccgcgc tgccagctcg
    40801 gcctccagcc gttgtcggta atggtgcagg tttagcgcca gatgttgctg gcgactgatg
    40861 gcaatgccca ggccaattcc cagcaaggcc tgggtcgcga ggaaaatctc cagctccacc
    40921 aggttactaa agcccacgcc aacctggcgg gcgatggtga tcatcatgct gcccaaaagc
    40981 ccggagagta cgccaccttg ccagccaaat ttccacgcca tcaccacatt gggcaaaaag
    41041 accacgatca acagcagacg ttcaatttca ggtgacagca ccatctgagt gccgatacca
    41101 ataataaaaa acaggctaca ccagatgatt aaagaggtac gcaagggggg attatgcgta
    41161 tcgagtccca ggagctggta gcgatgctgc tggcgcagaa actcaaagat aagatagaca
    41221 aacggcgtca gtaatacgcc gccggtaaat gaggtcaaac ccggcagcat tattgggcct
    41281 gacataaaag gggagagcag ggcggtattc aacaatgaag ctgccgtgac agtggctaac
    41341 agcaacgtca gacgctgcca gtaaagtgga aaacggtgcc agaagttttg tgcaaggagg
    41401 gcaggaaaca ggctaataac cggcgttgcc agcatgagat aaccgttcaa tagctgttca
    41461 ctatggagcc agaacatcat cagcaaaggg gggagcacca acgcaggcca gtagcggcga
    41521 gagagcaaaa taacaagggc gagataaact ccctgcggga gaaacaacac cgcctgttga
    41581 ccgttattgg tcagataaaa gctgagcgtc cacagcatca gccagccggt tccccaggcc
    41641 agcacaataa acagcgaaat cacccaatga cgaagattgc gcgacattaa tgccccgcca
    41701 gcagatggtg gtcgagcgca aaatgtacca gttcaatggt gctatggcag ttcagttttc
    41761 ccagcacatt ggcgcgatgc acatgtaccg ttttatgact gagttcgagt ttaaaggcga
    41821 tctctttaac gctgtctcct ttgaccagta aatcaaacac ttcacgttct cgtggggtga
    41881 gaatttccag cgcctgggcg ggttgcccgc cgccgcgtaa agcccgaatc gcatcggcac
    41941 agagataatg cccgcccagc ccgaccgagc gcaccgcctg tactaactct tccggcccac
    42001 agcgtttggt taaatacccg ctggcaccgg catccagcgc gctttgcaca aaggttggcg
    42061 cgtcataaat gctaagaata atggcgcgaa attgcggttt ttgtgcacgc aggcgcttaa
    42121 gcaaactgag gccgttttcg tccggcatgg caatatcaat gacggcaaca ttgacgtcat
    42181 cacggattaa tgccgaccat gcctgcgcgg cactactgta ctgaccaatg acctccagat
    42241 cgtcttccag agagagtaat tgtgcgaagc cagagcgcac caccacatgg tcatccacca
    42301 gcactacccg aatcatactt ttcttctctt attatctaat tctcctatca tgcatcacgg
    42361 cgcggcgctg gcaatcaacc agcgacagat atggaatgga tgtaacaaaa tggcaataat
    42421 ttgccggagc gcgcacatct tatctggctt gtgaaccggc gggatacgtt cgccaaaacc
    42481 gcaaaatgcg caaactcgct aaccattgcc gatttttttc taaaaacgct ttgttcttaa
    42541 caagaccttt taattccttt atcaaactga cccgcaacga aatactgctc ccataacaag
    42601 acaggggagc agacaatcat ggcaatttca tcgcgtaaca ctcttcttgc cgcactggca
    42661 ttcatcgctt ttcaggcgca ggcggtgaac gtcaccgtgg cgtatcaaac ctcagccgaa
    42721 ccggcgaaag tggctcaggc cgacaacacc tttgctaaag aaagcggagc aaccgtggac
    42781 tggcgtaagt ttgacagcgg agccagcatc gtgcgggcgc tggcttctgg cgacgtgcaa
    42841 atcggcaacc ttggttccag cccgttagcg gttgcagcca gccaacaggt gccgattgaa
    42901 gtcttcttgc tggcgtcaaa actgggtaac tccgaagcgc tggtggtaaa gaaaactatc
    42961 agcaaaccgg aagatctgat tggcaaacgc atcgccgtac cgtttatctc caccacccac
    43021 tacagcctgc tggcggcgct gaaacactgg ggcattaaac ccgggcaagt ggagattgtg
    43081 aacctgcagc cgcccgcgat tatcgctgcc tggcagcggg gagatattga tggtgcttat
    43141 gtctgggcac cggcggttaa cgccctggaa aaagacggca aggtgttgac cgattctgaa
    43201 caggtcgggc agtggggcgc gccaacgctg gacgtttggg tggtgcgcaa agattttgcc
    43261 gagaaacatc ctgaggtcgt gaaagcgttc gctaaaagcg ccatcgatgc tcagcaaccg
    43321 tacattgcta acccagacgt gtggctgaaa cagccggaaa acatcagcaa actggcgcgt
    43381 ttaagcggcg tgccggaagg tgacgtgccg gggctggtga aggggaatac ctatctgacg
    43441 ccgcagcaac aaacggcaga actgaccgga ccggtgaaca aagcgatcat cgacaccgcg
    43501 cagtttttga aagagcaggg caaggtcccg gctgtagcga atgattacag ccagtacgtt
    43561 acctcgcgct tcgtgcaata aaaggaggcg cagatgctgc aaatctctca tctttacgcc
    43621 gattatggcg gcaaaccggc actggaagat atcaacctga cgctggaaag cggcgagcta
    43681 ctggtggtgc tggggccgtc cggctgcggt aaaaccaccc tgctgaatct gattgccggt
    43741 tttgtgcctt atcagcatgg cagcattcaa ctggcgggta agcgtattga gggaccggga
    43801 gcagagcgtg gcgtagtttt tcagaatgaa gggctactac cgtggcgcaa tgtacaggac
    43861 aacgtggcgt tcggcctgca attggcaggt atagagaaaa tgcagcgact ggaaatcgcg
    43921 caccagatgc tgaaaaaagt ggggctggaa ggcgcagaaa aacgctacat ctggcagctt
    43981 tccggtggtc aacgtcagcg ggtgggcatt gcccgcgcgc tggcggcgaa tccccagctg
    44041 ttattactcg atgaaccgtt tggtgcgctg gacgccttca cccgcgacca gatgcaaacc
    44101 ctgctgctga aactctggca ggagacgggc aagcaggtgc tgttgattac ccacgatata
    44161 gaagaagcgg tgtttatggc gactgaactg gttctgcttt catccggccc tggacgtgtg
    44221 ctggagcggc tgccgctcaa ctttgctcgc cgctttgttg cgggagagtc gagccgcagc
    44281 atcaagtccg atccacaatt catcgccatg cgcgaatatg ttttaagccg cgtatttgag
    44341 caacgggagg cgttctcatg agtgtgctca ttaatgaaaa actgcattcg cggcggctga
    44401 aatggcgctg gccgctctcg cgtcaggtga ccttaagcat tggcacgtta gcggttttac
    44461 tcactgtatg gtggacggtg gcggcgctgc aactgattag cccgctgttt ttgccgccgc
    44521 cgcaacaagt actggcaaaa ctgctcacca ttgccggacc gcagggattt atggacgcca
    44581 cgctgtggca gcatctggca gccagcctga cgcgcatttt gctggcgcta ctcgcggcgg
    44641 tgttgatcgg tattccggtc gggatcgcca tggggctaag cccgacggtg cgcggcattc
    44701 tggatccgat aatcgagctt tatcgtccgg tgccaccgct ggcttatttg ccgctgatgg
    44761 tgatttggtt tggtattggt gaaacctcga agatcttact gatctattta gcgatttttg
    44821 caccggtggc gatgtcggcg ctggcggggg tgaaaagcgc gcagcaggtt cgcattcgtg
    44881 ctgcccagtc gctgggtgcc agccgtgcgc aggtgctgtg gtttgtcatt ttgcccggcg
    44941 cactgccgga aatcctcacc ggattacgta ttggtctggg tgtgggctgg tctacgctgg
    45001 tggcggcgga gctgattgcc gcgacgcgcg gtttaggatt tatggttcag tcagcgggtg
    45061 aatttctcgc gactgacgtg gtgctggcgg ggatcgcggt gattgcgatt atcgcctttc
    45121 ttttagaact gggtctgcgc gcgttacagc gccgcctgac gccctggcat ggagaagtac
    45181 aatgagtgaa cgtctgagca ttaccccgct ggggccgtat atcggagcac aaatttcggg
    45241 tgccgacctg acgcgcccgt taagcgataa tcagtttgaa caactttacc atgcggtact
    45301 gcgccatcag gtggtgtttc tgcgcgatca agccattacg ccgcagcagc aacgcgcgct
    45361 ggcccagcgt tttggcgaac tgcatattca ccctgtttac cctcatgccg aaggggttga
    45421 cgagatcatc gtgctggata cccataacga taatccgcca gataacgaca actggcatac
    45481 cgatgtgaca tttattgaaa cgccacccgc aggggcgatt ctggcagcga aagagttacc
    45541 ttcgaccggc ggcgatacgc tctggaccag cggtattgcg gcctatgagg cgctctctgt
    45601 tcccttccgc cagctgctga gcgggctgcg tgcggagcat gattttcgta aatcgttccc
    45661 ggaatacaaa taccgcaaaa ccgaagagga acatcaacgc tggcgcgagg cggttgcgaa
    45721 aaatccgccg ttgctacatc cggtggtgcg aacgcatccg gtgagcggta aacaggcgct
    45781 gtttgtgaat gaaggcttta ctacgcgaat tgttgatgtc agcgagaaag agagcgaagc
    45841 cttgttaggc tttttattcg cccatatcac taaaccggag tttcaggtgc gctggcgctg
    45901 gcaaccgaat gatattgcga tttgggataa ccgtgtgact cagcactatg ccaatgcgga
    45961 ttacctgcca cagcggcgga ttatgcatcg ggcgacgatt cttggggata aaccgtttta
    46021 tcgggcgggg taatacaaga gtggacggtc ccctcgcccc cttggggaga gggttagggt
    46081 gagggggcgt tcaccgtact ttcaacaggt taactccccc tttctgagag gaaacaaaat
    46141 taacgcagaa tcttcttctc agccaaatcc agcgcaaagt agctgaaaat cagatccgca
    46201 cccgcacgct taatcgaacc taagctttcg agcaccactt tctcttcatc tatagcaccc
    46261 gccagcgcgg cgaacttaat catcgcgtac tcaccgctca cctgatacgc gccaatcggc
    46321 aattcagtac gttcacgcag ctcacgcacg atgtcgaggt acgctccagc aggtttaacc
    46381 atcaggcagt ctgcgccctg ggcttcatcc agcagtgact cacgaatcgc ctcacgacgg
    46441 ttcattgggt tcatctgata gcttttgcgg tcgcctttta atgcgcttcc ggcagcttca
    46501 cggaacggac cataaaagga ggaggcgaac ttggtcgaat acgacataat cgccgtatct
    46561 ttaaagccag cagcgtccag cgcctggcga atcgcctgta cctggccatc catcgcggcg
    46621 gaaggggcga tgaagtctgc acctgcagca gctgcaacca cggcttgctt gcctaaattt
    46681 tccagagtcg cgtcgttatc gacgccatgc tcgcacagca caccgcagtg accgtgagaa
    46741 gtgtattcac agaagcaggt gtctgacatg acgatcattt ctggcacggt ctgcttgcag
    46801 atgcgcgaca tacgcgccac cagtccatct tcccgccagg catcgctgcc ggtttcatcg
    46861 gtatggtgag agatgccaaa agtcatcacg gaacgaatac cggcgttggc gatgcgttca
    46921 atttcgcgtg ccagatgttt ctctggaatg cgcatcacgc ctggcatggc ttcaacggct
    46981 ttgtagtcgt caatttcttc ttcaacaaag atcggcaaca ccaggtcgtt aaggctaagt
    47041 gttgtctctt caaacatagc gcgcagcgca ggagatttgc gcaggcgacg agggcgttgg
    47101 attaagtctg tcatggtctg cctgatgttt gtggaatcga ggggcatagt atacctgaag
    47161 cagggtaggg atgttttacg aaagttgtcg cgatgttgac aagaagagaa tggaagag
//
LOCUS       DAZUGK010000012        45767 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZUGK010000012 DAZUGK010000000
VERSION     DAZUGK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.012"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..245
                     /locus_tag="ACHY5W_000590"
     CDS             <1..245
                     /locus_tag="ACHY5W_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS4445551.1"
                     /translation="RAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQA
                     NAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            246..1304
                     /gene="rsxD"
                     /locus_tag="ACHY5W_000591"
     CDS             246..1304
                     /gene="rsxD"
                     /locus_tag="ACHY5W_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFS4445552.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNLPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1308..1928
                     /gene="rsxG"
                     /locus_tag="ACHY5W_000592"
     CDS             1308..1928
                     /gene="rsxG"
                     /locus_tag="ACHY5W_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFS4445553.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1932..2627
                     /gene="rsxE"
                     /locus_tag="ACHY5W_000593"
     CDS             1932..2627
                     /gene="rsxE"
                     /locus_tag="ACHY5W_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFS4445554.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2627..3262
                     /gene="nth"
                     /locus_tag="ACHY5W_000594"
     CDS             2627..3262
                     /gene="nth"
                     /locus_tag="ACHY5W_000594"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4445555.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            3873..5375
                     /gene="dtpA"
                     /locus_tag="ACHY5W_000595"
     CDS             3873..5375
                     /gene="dtpA"
                     /locus_tag="ACHY5W_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFS4445556.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            5480..6085
                     /gene="gstA"
                     /locus_tag="ACHY5W_000596"
     CDS             5480..6085
                     /gene="gstA"
                     /locus_tag="ACHY5W_000596"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFS4445557.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6129..6992)
                     /gene="pdxY"
                     /locus_tag="ACHY5W_000597"
     CDS             complement(6129..6992)
                     /gene="pdxY"
                     /locus_tag="ACHY5W_000597"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFS4445558.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(7051..8325)
                     /gene="tyrS"
                     /locus_tag="ACHY5W_000598"
     CDS             complement(7051..8325)
                     /gene="tyrS"
                     /locus_tag="ACHY5W_000598"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4445559.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(8454..9110)
                     /gene="pdxH"
                     /locus_tag="ACHY5W_000599"
     CDS             complement(8454..9110)
                     /gene="pdxH"
                     /locus_tag="ACHY5W_000599"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFS4445560.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPAEPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(9169..9498)
                     /gene="mliC"
                     /locus_tag="ACHY5W_000600"
     CDS             complement(9169..9498)
                     /gene="mliC"
                     /locus_tag="ACHY5W_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFS4445561.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(9596..10705)
                     /gene="anmK"
                     /locus_tag="ACHY5W_000601"
     CDS             complement(9596..10705)
                     /gene="anmK"
                     /locus_tag="ACHY5W_000601"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFS4445562.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLLVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            10979..11446
                     /gene="slyB"
                     /locus_tag="ACHY5W_000602"
     CDS             10979..11446
                     /gene="slyB"
                     /locus_tag="ACHY5W_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFS4445563.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNSRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(11492..11926)
                     /gene="slyA"
                     /locus_tag="ACHY5W_000603"
     CDS             complement(11492..11926)
                     /gene="slyA"
                     /locus_tag="ACHY5W_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFS4445564.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEYNIIELQAKG"
     gene            12127..12363
                     /gene="ydhI"
                     /locus_tag="ACHY5W_000604"
     CDS             12127..12363
                     /gene="ydhI"
                     /locus_tag="ACHY5W_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFS4445565.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12366..13223
                     /gene="ydhJ"
                     /locus_tag="ACHY5W_000605"
     CDS             12366..13223
                     /gene="ydhJ"
                     /locus_tag="ACHY5W_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS4445566.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            13223..15235
                     /gene="ydhK"
                     /locus_tag="ACHY5W_000606"
     CDS             13223..15235
                     /gene="ydhK"
                     /locus_tag="ACHY5W_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4445567.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMLA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDEHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(15236..15757)
                     /gene="sodC"
                     /locus_tag="ACHY5W_000607"
     CDS             complement(15236..15757)
                     /gene="sodC"
                     /locus_tag="ACHY5W_000607"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS4445568.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(15838..16734)
                     /gene="ydhF"
                     /locus_tag="ACHY5W_000608"
     CDS             complement(15838..16734)
                     /gene="ydhF"
                     /locus_tag="ACHY5W_000608"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4445569.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(16783..17022)
                     /gene="ydhL"
                     /locus_tag="ACHY5W_000609"
     CDS             complement(16783..17022)
                     /gene="ydhL"
                     /locus_tag="ACHY5W_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFS4445570.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            17125..17724
                     /gene="nemR"
                     /locus_tag="ACHY5W_000610"
     CDS             17125..17724
                     /gene="nemR"
                     /locus_tag="ACHY5W_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFS4445571.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            17761..18858
                     /gene="nemA"
                     /locus_tag="ACHY5W_000611"
     CDS             17761..18858
                     /gene="nemA"
                     /locus_tag="ACHY5W_000611"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFS4445572.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            18939..19346
                     /gene="gloA"
                     /locus_tag="ACHY5W_000612"
     CDS             18939..19346
                     /gene="gloA"
                     /locus_tag="ACHY5W_000612"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4445573.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            19449..20096
                     /gene="rnt"
                     /locus_tag="ACHY5W_000613"
     CDS             19449..20096
                     /gene="rnt"
                     /locus_tag="ACHY5W_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFS4445574.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            20189..24805
                     /gene="lhr"
                     /locus_tag="ACHY5W_000614"
     CDS             20189..24805
                     /gene="lhr"
                     /locus_tag="ACHY5W_000614"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4445575.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSPVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPTHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(24856..25203)
                     /gene="grxD"
                     /locus_tag="ACHY5W_000615"
     CDS             complement(24856..25203)
                     /gene="grxD"
                     /locus_tag="ACHY5W_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFS4445576.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            25538..26365
                     /gene="mepH"
                     /locus_tag="ACHY5W_000616"
     CDS             25538..26365
                     /gene="mepH"
                     /locus_tag="ACHY5W_000616"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFS4445577.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKLAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            26493..27074
                     /gene="sodB"
                     /locus_tag="ACHY5W_000617"
     CDS             26493..27074
                     /gene="sodB"
                     /locus_tag="ACHY5W_000617"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFS4445578.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(27220..28389)
                     /gene="ydhP"
                     /locus_tag="ACHY5W_000618"
     CDS             complement(27220..28389)
                     /gene="ydhP"
                     /locus_tag="ACHY5W_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4445579.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(28555..28644)
                     /gene="cydH"
                     /locus_tag="ACHY5W_000619"
                     /gene_synonym="ynhF"
     CDS             complement(28555..28644)
                     /gene="cydH"
                     /locus_tag="ACHY5W_000619"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFS4445580.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            28943..29968
                     /gene="purR"
                     /locus_tag="ACHY5W_000620"
     CDS             28943..29968
                     /gene="purR"
                     /locus_tag="ACHY5W_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFS4445581.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(29965..30897)
                     /gene="punR"
                     /locus_tag="ACHY5W_000621"
     CDS             complement(29965..30897)
                     /gene="punR"
                     /locus_tag="ACHY5W_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFS4445582.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            31010..32221
                     /gene="punC"
                     /locus_tag="ACHY5W_000622"
     CDS             31010..32221
                     /gene="punC"
                     /locus_tag="ACHY5W_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFS4445583.1"
                     /translation="MQPGKRFLVWLAGLSILGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCKEVGCQNH
                     GNAEVAHSESH"
     gene            32512..33660
                     /gene="cfa"
                     /locus_tag="ACHY5W_000623"
     CDS             32512..33660
                     /gene="cfa"
                     /locus_tag="ACHY5W_000623"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFS4445584.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(33700..34341)
                     /gene="ribE"
                     /locus_tag="ACHY5W_000624"
     CDS             complement(33700..34341)
                     /gene="ribE"
                     /locus_tag="ACHY5W_000624"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFS4445585.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTAGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            34556..35929
                     /gene="mdtK"
                     /locus_tag="ACHY5W_000625"
     CDS             34556..35929
                     /gene="mdtK"
                     /locus_tag="ACHY5W_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFS4445586.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(35970..37226)
                     /gene="ydhQ"
                     /locus_tag="ACHY5W_000626"
     CDS             complement(35970..37226)
                     /gene="ydhQ"
                     /locus_tag="ACHY5W_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS4445587.1"
                     /translation="MGSDAKNLMSDGNVQIVKIGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYDADLQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            37534..37610
                     /locus_tag="ACHY5W_000627"
     tRNA            37534..37610
                     /locus_tag="ACHY5W_000627"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37568..37570,aa:Val,seq:gac)
     gene            37615..37691
                     /locus_tag="ACHY5W_000628"
     tRNA            37615..37691
                     /locus_tag="ACHY5W_000628"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37649..37651,aa:Val,seq:gac)
     gene            37799..38104
                     /gene="ydhR"
                     /locus_tag="ACHY5W_000629"
     CDS             37799..38104
                     /gene="ydhR"
                     /locus_tag="ACHY5W_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFS4445588.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTQS
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVIAKVFDVNEPLSQINQAKLA"
     gene            38230..39834
                     /gene="ydhS"
                     /locus_tag="ACHY5W_000630"
     CDS             38230..39834
                     /gene="ydhS"
                     /locus_tag="ACHY5W_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFS4445589.1"
                     /translation="MKKIAIVGAGPTGIYTLSSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEAIRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTAQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASLARRRLSFD"
     gene            complement(39846..40658)
                     /gene="ydhT"
                     /locus_tag="ACHY5W_000631"
     CDS             complement(39846..40658)
                     /gene="ydhT"
                     /locus_tag="ACHY5W_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFS4445590.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(40662..41447)
                     /gene="phsC"
                     /locus_tag="ACHY5W_000632"
     CDS             complement(40662..41447)
                     /gene="phsC"
                     /locus_tag="ACHY5W_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFS4445591.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GQHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(41444..42112)
                     /gene="ydhX"
                     /locus_tag="ACHY5W_000633"
     CDS             complement(41444..42112)
                     /gene="ydhX"
                     /locus_tag="ACHY5W_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS4445592.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(42176..42814)
                     /gene="ydhW"
                     /locus_tag="ACHY5W_000634"
     CDS             complement(42176..42814)
                     /gene="ydhW"
                     /locus_tag="ACHY5W_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFS4445593.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(42827..44929)
                     /gene="ydhV"
                     /locus_tag="ACHY5W_000635"
     CDS             complement(42827..44929)
                     /gene="ydhV"
                     /locus_tag="ACHY5W_000635"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFS4445594.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPLHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(44950..45576)
                     /gene="ydhY"
                     /locus_tag="ACHY5W_000636"
     CDS             complement(44950..45576)
                     /gene="ydhY"
                     /locus_tag="ACHY5W_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFS4445595.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT        10998 a        11951 c        11321 g        11497 t
ORIGIN      
        1 cccgtgccaa agcgcgcaaa ctggaacagc aacaggctaa tgcggagcca gaagaacagg
       61 ttgatccgcg caaagctgcc gtcgaagcgg ctattgcacg cgccaaagca cgcaagctgg
      121 aacagcaaca ggctaatgca gaaccagaag aacaggttga tccgcgcaaa gcggcagttg
      181 ccgcggctat tgcccgcgtt caggccaaaa aagccgtcca gcaaaaggtt gtaaacgagg
      241 actaaatggt attcagaata gctagctccc cttataccca taaccagcgc cagacatcgc
      301 gcattatgct gttggtgttg ctcgcagccg tgccaggaat cgcagcgcaa ctgtggtttt
      361 ttggttgggg tactctcgtt cagatcctgt tggcgtcggt cagtgctctg ttagccgaag
      421 ctctcgtact caaactacgc aaacagtcgg tagccgccac gttgaaagat aactcagcat
      481 tgctgacagg cttattgctg gcggtaagta ttccccccct cgcgccatgg tggatggtcg
      541 tgctgggtac ggtgtttgcg gtgattatcg ctaaacagtt atatggcggt ctgggacaaa
      601 acccgtttaa tccggcaatg attggttatg tggtcttact gatctccttc ccggtgcaga
      661 tgaccagctg gttaccgcca catgaaattg cggtcaacct ccctggtttt atcgacgcca
      721 tacaggttat tttcagcggg cataccgcca gtggtggtga tatgaacaca ctacgcttag
      781 gtattgatgg cattagtcag gcgacaccgc tggatacatt taaaacctct gtccgtgccg
      841 gtcattcggt tgaacagatt atgcaatatc cgatctacag cggtattctg gcgggcgctg
      901 gttggcaatg ggtaaatctc gcctggctgg ctggcggcgt gtggttgcta tggcagaaag
      961 cgattcgctg gcatattccc ctcagcttct tagtaacgct gacgttatgc gcaacgttgg
     1021 gctggttgtt ctcaccagaa acactggcag caccgcaaat tcatctgctg tctggagcga
     1081 ccatgctcgg cgcattcttt attttgactg acccggttac cgcttctacg accaatcgtg
     1141 gtcgtcttat tttcggcgcg cttgcgggct tattagtctg gttgatccgc agtttcggcg
     1201 gctatcctga cggcgtggct tttgccgtcc tgctggcgaa catcacggtt cctctgatcg
     1261 attactacac gcgtccgcgc gtctacggcc atcgcaaagg gtaaaccatg ctgaaaacta
     1321 tccgaaaaca cggcattacg ttggcgctat ttgcagcggg ttcaacaggg ttaactgcgg
     1381 ccatcaacca gatgaccaaa acgacgattg ctgaacaggc cagtctgcaa caaaaggctt
     1441 tatttgatca ggtgctgcca gccgaacgct ataacaatgc gctggcacag agttgctatc
     1501 tggtaactgc gccagagtta ggtaaaggtg agcatcgggt ttacatcgcc aaacaggatg
     1561 acaaaccggt agccgccgtt ctggaagcaa ccgcgccaga tggctattcc ggtgcgattc
     1621 agctgctggt gggagccgat tttaacggca cggtacttgg cacgcgcgtg acagagcacc
     1681 acgaaacgcc agggcttggc gataaaatcg aactgcgcct ttctgactgg atcacccatt
     1741 ttgcgggtaa aaaaatcagt ggtgcggatg atgcgcactg ggcggtgaag aaagatggtg
     1801 gtgatttcga ccagttcacc ggcgcaacga ttactccccg cgcggtggtt aatgcggtaa
     1861 aacgcgccgg attgtacgct cagacgttac cggcacaact ttctcaactt cctgcctgtg
     1921 gagaataaag cgtgagcgaa attaaagacg ttattgttca ggggttgtgg aaaaacaact
     1981 ctgcgctggt ccagttgctc ggcctttgtc ctctgttggc ggtcacgtcc actgccacta
     2041 acgctctggg tttaggactt gcgactacgc tggtactgac gctgaccaac ctgaccattt
     2101 cgacgctgcg tcactggacg ccagccgaga tccgcattcc catttacgtg atgatcatcg
     2161 cctcggtggt cagcgctgta cagatgctga tcaacgccta cgcctttggc ctgtatcaat
     2221 cattagggat ttttattccg ctgattgtca ctaactgtat cgttgtgggc cgcgctgaag
     2281 ccttcgccgc caaaaaaggt ccggcgcttt cggcactgga cggcttttca attggtatgg
     2341 gcgcaacctg cgccatgttc gtgctgggtt cactacgcga aattatcggc aatggcacat
     2401 tgtttgacgg tgcagatgcg ctgttaggta gctgggcaaa agtattacgc gtggagattt
     2461 tccacaccga ctcccctttc ctgctggcga tgctgccacc aggtgcattt attggcctgg
     2521 gactgatgct ggcaggaaaa tacctgattg atgaaagaat gaaaaagcgc cgtactgaag
     2581 cagctgccga acgtgcattg ccaaacggtg aaacagggaa tgtctgatga acaaagcaaa
     2641 acgcctggag atcctcactc gcctgcgtga gaacaatcct catcccacca ccgagcttaa
     2701 tttcagttcg ccttttgaat tgctgattgc cgtactgctt tccgctcagg cgaccgatgt
     2761 cagtgttaat aaggcgacgg cgaaactcta cccggtggcg aatacgcctg cagcgatgct
     2821 tgaactgggc gttgaagggg tgaaaaccta tatcaaaacg attgggcttt ataacagcaa
     2881 agcagaaaat atcatcaaaa cctgccgtat cttactggag cagcataatg gcgaggttcc
     2941 ggaagatcgt gctgcgcttg aagccctgcc cggcgtaggt cgtaaaacag ccaacgtcgt
     3001 attaaacact gcattcggct ggccgactat tgctgtcgac acgcacattt tccgcgtttg
     3061 taatcgtact caatttgcgc cggggaaaaa cgtcgaacag gtagaagaaa agctactgaa
     3121 agtggttccg gcagagttta aagtcgactg ccaccattgg ttgatcctgc acgggcgtta
     3181 tacctgcatt gcccgcaagc cccgctgtgg ctcttgtatt attgaagatc tttgtgaata
     3241 caaagagaaa gttgacatct gaagaaaagg ggtaacaccg attaccccat tgataacctt
     3301 tctttatcct cttttaaaac atctttttaa cgtcaatgat gccattgctt agcgttatca
     3361 tcaggtaatc cgtttgcgga taaccaaaaa tgcaggttaa ttgttttttt aatagcgaaa
     3421 ttttctattc attcgtgatc aagatcacga tgctgtggca atgtaaaaac attgcaagaa
     3481 tcaggttaaa atcatttaaa tttacacacg caacaaatat tgacctacaa aacattacac
     3541 tggctatttt tcagaaactg gactatctca ctagtaaaaa cgctaaatag cagaacatat
     3601 cgccgtcaag ccatcatccc cctctcaatg cagtgaaaaa atctgccgtt acgttttttg
     3661 aaaaatttaa cgctggataa catttcccgg aatggttgaa ttccccgcct cagttatatg
     3721 taacagatta ttacaaagga cttgtctgaa agtgcaagat agtgaacatt acctgccgtt
     3781 tcccctccca ctataacaat tgcgcgtatg ttttttaggc ataacgcgag aaagcacccc
     3841 cgttaatatg ggatgtaaaa aaagaggtaa aagtgtccac tgcaaaccaa aaaccaactg
     3901 aaagcgtcag tttgaacgct ttcaaacaac cgaaggcgtt ctatctcatc ttctcgattg
     3961 agttatggga acgttttggt tattacggcc tacaaggaat tatggctgtt tacctggtta
     4021 aacaactggg tatgtctgaa gcggattcaa tcaccctttt ctcttccttt agtgccctgg
     4081 tttatggtct ggtcgctatc ggcggctggt taggtgacaa ggtactgggt actaaacgcg
     4141 taattatgct cggcgctatt gtgctggcga ttggttatgc tctggttgcc tggtctggtc
     4201 acgacgccgg tatcgtttat atgggtatgg cggctattgc ggtcggtaac ggcctgttta
     4261 aagctaaccc gtcttctctg ctttctacat gctatgagaa aaacgacccg cgtctggacg
     4321 gtgcattcac catgtactac atgtccgtca acatcggctc tttcttctct atgatcgcca
     4381 ccccgtggct ggccgcgaaa tacggctgga gtgttgcgtt tgcgttgagc gttgtaggcc
     4441 tgctgatcac tatcgttaac ttcgccttct gccaacgctg ggttaaacag tacggttcaa
     4501 aaccagattt cgagcctatc aactaccgta acctgctgct gaccattatt ggtgttgtgg
     4561 cactgatcgc tatcgccacc tggctgctgc acaatcagga agttgcgcgt atggcgctgg
     4621 gcgttgttgc cttcggtatc gtggtgatct tcggtaaaga agccttcgcg atgaaaggtg
     4681 ctgcgcgtcg taaaatgatc gttgccttca tcctgatgct cgaagccatt atcttcttcg
     4741 tgctgtacag ccagatgcca acgtcactga acttctttgc gattcgtaac gttgaacaca
     4801 ccattctggg cctggccgta gaacctgagc agtatcaggc actgaacccg ttctggatca
     4861 tcattggtag cccgattctg gccgctatct ataacaagat gggcgatacc ctgccgatgc
     4921 caactaagtt tgcaatcggt atggtgatgt gttctggtgc gttcctgatt ctgccgctgg
     4981 gtgcaaaatt cgcgtctgac gcaggtatcg tgtctgtaag ctggctggtc gcaagctatg
     5041 gcctgcagag tatcggggaa ctgatgatct ctggtctggg tctggcaatg gttgctcaac
     5101 tcgttccgca gcgtctgatg ggcttcatta tgggtagctg gttcctgacc actgccggtg
     5161 caaacctgat tggtggttat gttgcgggta tgatggctgt gccggataac gttaccgatc
     5221 cgctgatgtc actggaagtc tatggtcgcg tattcttgca gattggtgtc gctactgccg
     5281 ttattgcagt actgatgctg ctgaccgcgc cgaaactgca ccgcatgacg caggatgacg
     5341 ctgcagacaa agcggcgaaa gcagccgtag cgtaaaattc agggaaactc ttttacaagt
     5401 cgctaacttt tcgttagcgg cttttttttg ttcagcaacg cactaccata ctttaaacca
     5461 cagacaaaag gagttaccga tgaaattgtt ctacaaaccg ggcgcctgct ctctcgcttc
     5521 ccatatcacc ctgcgtgaga gcggaaagga ttttacgctc gtcagtgtgg atttaatgaa
     5581 aaaacgtctc gaaaacggtg acgattactt tgccgttaac cctaaggggc aggtgcctgc
     5641 attgctgctg gacgatggta ctttgctgac ggaaggcgtt gcgattatgc agtatcttgc
     5701 cgacagcgtc cccgaccgcc agttgctggc accggtaaac agtatttccc gctataaaac
     5761 catcgaatgg ctgaattaca tcgccaccga gctgcataaa ggtttcacac ctctgtttcg
     5821 ccctgataca ccggaagagt acaaaccgac agttcgcgcg caactggaga agaagctgca
     5881 atatgtgaac gaggcactga aggatgagca ctggatctgc gggcaaagat ttacaattgc
     5941 tgatgcctat ctgtttacgg tactgcgctg ggcatacgcg gtgaaactga atctggaagg
     6001 gttagagcac attgcggcat ttatgcaacg tatggctgaa cgtccggaag tacaagacgc
     6061 gctgtcagcg gaaggcttaa agtaatagcc atcggcccac agatcgttgt gggccgaaat
     6121 gagatatttc agagctttgt tgcgctgaag taatgttctg gtttggcaat acgatcctga
     6181 gcagccacca cttgcagctc atattcctgc attgctttgg tggtcaccat gatttcgtag
     6241 actgcagcgg tcacatgttc cagggcctcc tgcagcgttg ccccctgaag cagtttcacc
     6301 agcagtaaac cgctcgtcac atcaccaaca cctaccggct ggcgcatacc aaaatccacc
     6361 agtggccggc tgatatgcca ggcttcatcg gcggtgacca gcagcatttc aaaacggtca
     6421 cggctgtagc cagctcgcgc caggtgttta actaacacaa tttgtggccc ttgcgcaatg
     6481 agttcgcgcg ctgccagaac cgcttcttcg acgttattta ccgcatgctc acagagtatt
     6541 tccagctcaa ccagatttgg cgcaatgata tcgctggcag gcaaaccgtg ccgcacatga
     6601 aactctgcga cacccggtgc aacgatacag cctttttccg gatgacccat taccggatcg
     6661 caaaaatatt tcgcctgcgg attcgcggct ttcacctgac ggacgatacc gagaatatgt
     6721 tcaccctgct ccgccgatcc cagatagcca cttaatacgg catcacaggt gtgtaattta
     6781 tcaatggcgg caatgccttg cacaatttcg gttaaatggc tgggcggcat cacgcagcca
     6841 gtccatttgc cgtattgggt gtgattagaa aattgaacgg tgttcagcgg ccagacgttc
     6901 gcgcccaggc ggcgcatcgg aaactctgcc gcactgttac ccgcatgacc ataaacaacg
     6961 tgagactgga tagcgagaat atttttcatc atgttccctg tataaaaacc agggggagtg
     7021 atttctcact ccccctttcc acttaatgca ttatttccag caaatcagac agtaattctt
     7081 tttgccgcga cgcagtaagg taaaacgacc aaacagacga tcttcttctt taaagaagta
     7141 ttcaggatcg gactgttttt caccgttaat ggtgatggca ttggaggcga tagttttacg
     7201 cgcctgacca cgggaaggtt gcagttcaga atcgaccagc gcctgcatca ggtctgcgcc
     7261 cttttccatc tcaaccatcg gtacgccgtc ctgcgccagc tgttcgaagt ccgcttcact
     7321 cagcgcactc aaggaaccgc tgaacaggca ttcggtaata cgttttgccg cctgcaaacc
     7381 ttcttcaccg tgaaccagac gggtcacctg ctccgccagt acatactggg cgcgcggtgc
     7441 tttaccgctg tttttatctt cttcttccag ggcgttgatc tcttcaatgc tcataaaggt
     7501 gaagaacttc aggaagcggt aaacgtcggc atccgcagtg ttgatccaga actggtagaa
     7561 tttgtacggg ctggttttct tcggatccaa ccagactgcg ccgccttcag ttttaccaaa
     7621 tttggtgcca tctgctttgg tgatcagcgg aacggtcagg ccaaacacct gattctgatg
     7681 cagacgacgg gtcaggtcga taccagaagt gatgttaccc cactgatcag aaccaccaat
     7741 ttgcagcacc acaccgtact gtttgttcag acaggcgaag tcataaccct gcaacaggtt
     7801 gtaggaaaac tcagtgaacg aaatcccctg atcttcacgg ttgagacgct gcttaaccgc
     7861 ttctttgttg atcatctggt taacggagaa gtgtttgcca atatcgcgca ggaaggtcag
     7921 cacattcata ttgccgaacc agtcatagtt gttcgccgcg atagcagagt tttctccaca
     7981 gtcgaaatcg aggaacgggg caacctgctt acggattttg tccacccact cctgaacagt
     8041 ttcttcagtg ttcagcttac gctcggcagc tttgaagctc gggtcgccaa tcagacccgt
     8101 cgcgccgcct accagcgcaa ccggcttgtg gcccgcctgc tggaagcgtt tcaggcataa
     8161 caatggaaca agatgcccca aatgcaagct gtcagcggta ggatcgaagc cgcaatagag
     8221 cgcgatcggg ccttgcgcca gtcgctctgc taacgcttcc tcgtccgtca cctgggctac
     8281 cagcccccgc tcttgcaatt gtttaatcaa gttactgctt gccatcaaaa tctccatgta
     8341 tataacgact gcacctttgc cggtacacga cttttcgcca gatgcgaaag agacatagaa
     8401 taaagtgcca gaatcaggag taccagcgat taaagcaaga tttttgcatc ttttcagggt
     8461 gcaagacgat caatcttcca cgcatcattt tcacgctggt acaaaaagcg gtcatgcagg
     8521 cgatgctcac ccccctgcca gaactcaatc tgttcaaggc tgacgcgaaa accgccccaa
     8581 aagctcggca atggcacttc gccctgttga aacttctgct tcagctccag gaatttactt
     8641 tcaaggatac cgcgggcaga aatgcgactg gactgcttcg aaacccatgc accaatctgg
     8701 ctatcacgcg ggcggctatg aaaatatttc atcacttcga gagtcgaaag tcgttccgct
     8761 ttaccgatca ccatcacctg gcgctcaagg gtatgccacg ggaacagcag gctaacgcgc
     8821 ggattatttt cgatttgatg tgctttacgg ctgccgaggt tggtgtaaaa caccatgcct
     8881 ttttcgtcgt aatgtttgag taaaacgatg cgctgataag gctgaccatg ttcatccacg
     8941 gtagcgacca ccatcgcggt agggtccgcc agtttggctt cacaagcctg agagagccag
     9001 cgttcaaaaa gggttaatgg ttcggcagga agatcgcggc ggcgtaaccc gcctttggtg
     9061 tattcacggc gcagatgcgc gatttgctgc aattcgtcgt tatcagacat ggttttcttt
     9121 acggattgtc agtgggtgac gctattgtgc gccgcccctg gaaaaatctc aacgctgtgg
     9181 attttgtaac tgacagttat tcaatacgat gcggtcgcgt ttatagacag tcgcttcatc
     9241 gcctttcgac cagaaaacat agattccgtc agtgtaacgc gcgccagagg ctgaaatgcc
     9301 ctgtttgaga tgcagtagtt gattatcgta aacaaaactg acctcctggc gcggattatt
     9361 cagtttgacc gtcaacggtt tttcatcaca ctggtattcc agcgtatcgg tttgcatgcg
     9421 ctcaaccagc tgattgaacg cgctacagcc ggtgagaagc accggcaaac agatcagtaa
     9481 cagttttttc atagacatat cccgaagact ttcctggtct ggagggcaat acgccctccc
     9541 taacgttcca agtgtaacgg cagacgcggt aagaaaaatt cagttaactc tgatatcacg
     9601 ggttagcggg gaaaatagcc ccgagtaccg tctcctggct tgcgccagtg acggaaggca
     9661 gatttcctgg taatcccgcc agcgtacgcc aggcaagcca ggcgaaagcc aatgcttcca
     9721 tgtcatcgcc actaatgcca acggcatcgg tggtggtgac ttctgtgcct ggcagtaatg
     9781 ccgccagacg cgccatgagt agcggattac gacttcctcc accacatacc agcaatcgtt
     9841 cacagccacc gctcaacaaa acttgttcag aaatggtcac ggcggtgagt tctgccagtg
     9901 tggcctgcac atcacgggga tcaacacccg gaaaatggcg caaatggcgc tccagccagc
     9961 catagttaaa gtattcgcgt ccggtgcttt tcggtgcggg ttgcgagaaa tacgggtcgc
    10021 tgagcatatt ttgcagtagt gggagaataa ctttacccgc ccgtgcccac tcggcatctt
    10081 tatcgtaagg tttaccggcc tgacgccaga tccaggcatc catcagcatg ttaccaggac
    10141 cggtatcgta gcccccaacc ggctgcccag gaatgagcag tgacagattg gcgatgccgc
    10201 caatattgag caccattcgt cgctcggttg ggtgagccag caaggcatga tggaacgcag
    10261 gtaccagcgg tgcgccttgt cctcccaagg caatatcgcg acggcgaaaa tcaccgacaa
    10321 ccgtaattcc ggtgcgtgcc acaatttgat tattatcgcc aatctgtaaa gtgtgtggtg
    10381 ccacgcccgt cggttcatgc cagacggttt gaccgtgaca gccgatcgca actatatctc
    10441 tcgcctgcag gttttgttcc ttaagcaagg cattgaccgc atcagcaaaa agtcgcccga
    10501 gttgagtatc aagctgtcca aactgcgaaa gtgtaagctg ctggccctgg caaatatcca
    10561 gtacagcctg tttcagagac accgggatcg gccagctcaa acttgccagc tgtgcgaccc
    10621 ggtgttcatc aattgtcgcc aacacaacat caacaccatc aaggctggtg cctgacataa
    10681 cgccaataaa gcggcccgat ttcatagttc atcctttttc aatctgacgt ttgcgcacca
    10741 ctcaaacata aacttttcgt gaataccatg cggaatgacc gatttttacc gttggtagta
    10801 aaacattatc ttcaaatcaa taatcatcat gaatgttttg tttataattg gttgatccta
    10861 ctttcattat gatttgctca tatttggtag aacatgtaac catggattca catatgccat
    10921 atactttgac catgagggat gcttgcgtgg cgtttcatgg taaacaggag atttttcaat
    10981 gattaaacgc gtattggttg tttcaatggt aggtctgtct cttgtcggtt gtgttaataa
    11041 cgacaccctg tcaggggatg tttataccgc ttctgaagcg aaacaagtac agaatgtcag
    11101 ctatggcacc atcgttaacg tacgtccggt acagattcag ggcggtgatg attccaacgt
    11161 gatcggtgca attggcggtg ctgttcttgg tggtttcctg ggaaatactg ttggtggcgg
    11221 aaccgggcgt tctctggcta ctgcagcagg cgctgttgca ggtggcgtag ccggtcaggg
    11281 cgtacagagt gcaatgaaca aaacgcaggg tgtcgagctg gaaattcgta aagacgatgg
    11341 taataccatc atggtggtac agaaacaagg caactctcgt ttctctccgg gccaacgtgt
    11401 cgtactggcc agcaatggca gtcaggtgac cgtttctccg cgctaaatag tttgcgtgtg
    11461 gtcaggttac tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat
    11521 tatactcaag ttttgcgatg agcgtaatca gttgttccag ttcctctgcg gagatgccat
    11581 gtaaaatttc cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agcggctctg
    11641 ccttttccgt cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa
    11701 ttaacccttt ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa
    11761 tcgcttttgc cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg
    11821 taacccaatg ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc
    11881 atatgcgcac caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat
    11941 aattagcttg ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa
    12001 aacaaatgta ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa
    12061 tttttcgcac ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc
    12121 tactttgtga agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt
    12181 gcgtccgtct actttcctcc gtttttcaaa gccttcgcgt ttggattcgt catctggctt
    12241 gtcgtacacc gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttatta
    12301 atggatttat cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg
    12361 taactatgtc aattaaaaca attaagtatt tctcaacaat cattgtagcg atagttgcgg
    12421 ttcttgccgg atggtggttg tggaattatt acatgcaatc tccgtggaca cgcgacggga
    12481 aaatacgcgc agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga
    12541 atataaaaga caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc
    12601 cctttcagat cgccgagctg aatgcacagg ctcagctggc gaaagcgcaa tctgacctcg
    12661 caaaagccaa taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg
    12721 aagaactcga taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac
    12781 aggcgacgct gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga
    12841 gtggatgggt gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc
    12901 tttttgccct tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt
    12961 tgcgccatat ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt
    13021 tacagggtca cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact
    13081 caagccttat tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc
    13141 ccgtccgctt tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct
    13201 gtagcattgc cgtaggtcaa taatgaacgc atcgtcatgg tccttgcgca atttgccctg
    13261 gttcagggcc acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct
    13321 ggcgctgacg gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc
    13381 tgcagtggtt agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc
    13441 tggcagttta ctgggagcca ttgcggcact gcttcttgcc gggcatacgc tcaatgagcc
    13501 gtggtttttt ctattgagca tgttggcgtg gcttggcttt tgtacctggg cctgtgcgca
    13561 cttcacgaat aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc
    13621 ctttccgatg gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt
    13681 ttgcgaggtg attgtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag
    13741 ttccgatgct acagcccttt taaccgcatt gaaaaacatg cacgcccgat tactggaaca
    13801 tgccagttta ctctggcagc ctgaaacaac cgatgccatt cgtgcagcac atgaaggggt
    13861 gattgggcag atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg
    13921 ttttcgccag caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag
    13981 tgtcatctcc agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg
    14041 agaaattctc gaacagttgc tgacggcgct cgccagttcg caaacagatg tttacaccgt
    14101 cgcacgtatt atcgccccgc tacgcccgac caacgtcgcc gactatcggc acgtcgcctt
    14161 ctggcagcga ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg
    14221 tctgcaaagc ggtgtagatg aacatgccag actcccacgg acatccggcc tggctcgtca
    14281 taccgataac gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat
    14341 tggcgcatgg agtattgctt cgcaatggga tgccggtgcc aatgcattaa cgctggcagc
    14401 aattagctgc gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat
    14461 gcgcacgctg gtgttacttt cgctattcag ctttgtggtc aaatttggtc tgatggtcca
    14521 gattagcgat ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct
    14581 tcttaaattg cagatgccaa aatttgccgc actgtggggg caactgattg tttttatggg
    14641 ttcgtttatc gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgataa
    14701 tctggcgaaa atcgttggcg tcgcgttggc gtggttagcg ttcgccattc tgcgtccagg
    14761 atcggatgct cgtaaaagcc gccgccatat tcgcgcgctg cgtcgggatt ttgtcgatca
    14821 actaagccgc catccaacac tgagtgaaag cgaatttgaa tcgctcactt atcatcacgt
    14881 cagtcagttg agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt
    14941 agtgctgctg aactgttctc atgttgtctg gcaattacgc gactgggaat cgcgttccga
    15001 tccgttatcg cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg
    15061 tggcgttcag caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag
    15121 ccttgcccgt catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta
    15181 ctgctcgctt tcgcaacttg agcaagcacc accgcaaggt acgctggcct cttaattact
    15241 taattacacc acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca
    15301 tattatcgcc gccaacgtgg accatcagcg ctttgtcttt gatttcatcc agtgatttca
    15361 gacgaggcgc gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca
    15421 gatcgcctaa atgcccggca ccttctggcc cttcatgttt accggtattt tgtggatcaa
    15481 gatgcccgcc tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc
    15541 ttcctttggc atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggcg
    15601 aaaactccag acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc
    15661 cttgcgacgt gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa
    15721 caaccagcgc cagaatagcc agactaaaac gtttcatagg acctccgttc attgtgcatt
    15781 cctgaataag tgtaaaccag tgacacattt ttgaacggcg actattccta aaagtgctta
    15841 cggtacgtcg taacccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt
    15901 cagtgtttct gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg
    15961 cagcggctgc gatggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc
    16021 gtttaactcc tctgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt
    16081 aaacagacga ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgcag
    16141 ttggtcgagc gtgccatcca gcagtaacgg ctgatgtacc ggggatattt ccacctgatt
    16201 agtggcaagg gtaaacggca gacgtgattg caacagggca aattgcgcag gcgtaaagtt
    16261 cgatacgcca aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac
    16321 ttcatcggca tccattaacg ggtccgggcg gtggattaac agcagatcca gatgatcggt
    16381 cgcaagatta attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta
    16441 atgaccaatg acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc
    16501 catccgctca cgcaggtgag gtgccagttt cagcgcctcg ccaaacgccg cttcgcactg
    16561 atagccacca taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc
    16621 aataaaactg accagctggc gggcggacat gttccagtcc atcaatcgcc agtagcccat
    16681 cacaaaacgg gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc
    16741 ttcctcttat cagatatgag aggagtatac gcaagattag gctcaaaaga gtgatggttg
    16801 ctccggttcg tctgatgacg ctggcttatt tgcacgtaat ttgcgcatta atcgttgccg
    16861 acaaaggcgc agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc
    16921 atcccgacta cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg
    16981 gcacgggctc tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa
    17041 gattattacc attattgaag ctgttaatgc ccaaagtagc aactttgctt gcactagacc
    17101 gactggtcta ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat
    17161 ctcctggcga cgggcgagca actttgcctg caacgtggat tcaccgggat ggggctaagc
    17221 gaattactaa aaaccgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa
    17281 gaagcgtttg gcgtagccat gcttgagcgc cattacgccg catatcacca gcgactgact
    17341 gagttgctgc aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa
    17401 acactgaacc agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct
    17461 gccgaagtgt gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc
    17521 gtgatcgccc tgctctcgca ggctctggaa aatggccgtg atagccattg tttaaccttt
    17581 tgtggcgaac cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg
    17641 caggccaaaa tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc
    17701 attattgcga cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt
    17761 atgtcatctg aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt
    17821 atttttatgg caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg
    17881 ttgatggcgg aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg
    17941 caaatttctg cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa
    18001 attgccgcat ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta
    18061 cagctgtggc acaccggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg
    18121 gtagcgcctt cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag
    18181 gcgatccgtg ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc
    18241 gtcaatgatt tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag
    18301 ctccactctg ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt
    18361 accgatcagt acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat
    18421 gccgggattg aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact
    18481 ttccagaaca cagataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa
    18541 ctgggtaaac gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa
    18601 ccgtatactg atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc
    18661 gcaggtgcat acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg
    18721 gtggcatttg gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaaa
    18781 gctgagctta acccacagcg tgctgaaagt ttctatggtg gcggtgcgga aggctacact
    18841 gattacccga cgttgtaatc caacattgcg agcggcgcaa agccgccgct atactaaaac
    18901 aacattttga atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc
    18961 tgcgcgttgg cgatttgcaa cgctccatcg atttttatac caaagttctg ggcatgaaac
    19021 tgctgcgtac cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc
    19081 cggaaaccga agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac
    19141 tcggcactgc ttatggtcac atcgcgctta gcgtagataa tgccgctgaa gcgtgcgaaa
    19201 aaatccgtca aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta
    19261 cggttatcgc gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag
    19321 acgccggtcg cggtctgggc aactaatctc ctgccgggcg cgaactcatc gcgcccgcat
    19381 ctttactgca tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag
    19441 agaatcagat gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt
    19501 atcctgttgt gatcgatgtt gaaacagctg gatttaacgc caaaaccgat gcgctgcttg
    19561 agattgccgc catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat
    19621 tacatttcca cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca
    19681 acggcattga cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg
    19741 aaatttttaa agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg
    19801 tggcacacaa cgccaatttc gatcacagct ttatgatggc cgccgctgaa cgcgcctcac
    19861 tgaaacgtaa cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg
    19921 cactcggaca aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca
    19981 cccaggcgca ctccgcgctg tacgacaccg aacgcactgc ggtgctgttt tgtgaaatcg
    20041 tcaaccgctg gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga
    20101 gtcgatgcct gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca
    20161 tgcatgatat tcacggggaa tagcgttaat ggcagataat ccaaaccctt catcgcccct
    20221 gccggacgtg ttttcaccgg cgacccgcga ctggtttctt cgcgccttta aacagccgac
    20281 cgctgtccag tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc
    20341 accgaccggc tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg
    20401 cgaaggcggc gaagataccc gcgaggcgca taagcgtaaa acctcacgca ttctctatat
    20461 ttcaccgata aaagccctgg gcaccgacgt tcagcgcaac ttacagatcc cgttacaggg
    20521 tattgccgat gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tggggatccg
    20581 tactggcgat acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct
    20641 gatcaccaca cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg
    20701 cggcgtcgaa acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc
    20761 gcatctggcg ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat
    20821 tggcctttct gccactgtgc gctcagccag cgatgtggca gcatttcttg gcggcgatcg
    20881 cccggttacg gtagtcaacc cgcccgcaat gcgccatccg cagatacgga ttgtcgtacc
    20941 agtcgccaat atggatgatg tctcaccggt cgccagcggc actggcgaag acagccatgc
    21001 cggccgggaa ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg
    21061 ccatcgctcg accattgtct ttactaattc gcgggggctg gcggaaaaac tgacggcacg
    21121 attaaatgag ctttacgccg cacgcttaca gcgttccccg tctatcgccg ctgatgcggc
    21181 ccatttcgag tcgacctctg gcgcaacctc taaccgtgta caaagtagcg acgtttttat
    21241 tgcccgctca caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct
    21301 gaaatcgggt gagttacgct gcgtggtcgc aacttccagt ctcgaactgg ggattgatat
    21361 gggcgctgtg gatctggtga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca
    21421 acgcattgga cgtgccggac accaggtagg aggtatctcc aaagggcttt ttttcccccg
    21481 aacccggcgt gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct
    21541 ggaaaacctg acacctccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc
    21601 cgcggcgatg gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc
    21661 gtggaaagat ctgccaagac gtgtttttga cgccacgctg gatatgcttt ccgggcgcta
    21721 tccctctggc gatttttctg cttttcgacc aaaactggtc tggaacaggg agaccgggat
    21781 attgaccgcc cgacctggcg ctcagctgtt ggcagttacc agcggtggca ccattccgga
    21841 tcgtggcatg tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt
    21901 gggtgaactg gatgaggaga tggtatatga gtcgcgggtg aacgatatta tcactctcgg
    21961 cgcaacctca tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc
    22021 gggtcgttct gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt
    22081 aggcgagatg attggcgatt ttcttcattt gctggcggat ggagcgttct tttccgggac
    22141 tattcccccg tggctggctg aagaaaatac gatcgccaat attcaggggt tgattgacga
    22201 gcagcgcaac gcgacgggca tcgttccggg gagtcgtcat ctggtcctcg aacggtgccg
    22261 tgatgaaatt ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga
    22321 accttgggcg ctggcgattg ccgggcgaat acatgcgcta tggggcgctg atgcgtctgt
    22381 ggtcgccagt gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcctga
    22441 cgccgcgatt tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg
    22501 cagctcagca cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcgt tattaatgcc
    22561 gggccgcacg ccgggccatc gcaccccgct ctggcaacaa cggctgcgcg ccagtcagtt
    22621 gctggaaatc gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg
    22681 tctgcaagat gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg
    22741 cgaaattcaa atatccgatg taacgactac tacgccctcg cctttcgcca caagtttatt
    22801 gttcggctat gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc
    22861 atccgtactg tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg
    22921 ggaattactc gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc
    22981 tggcagaaga gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat
    23041 gaccgttgaa gacctggcgc aacggcatac aggcagcagt gaagagattg cgtcgtatct
    23101 ggaaaatctt cttacagtaa aacgaatctt cccggcgatg attagcggac aggagcgtct
    23161 tgcctgtatg gatgatgccg ccaggctgcg cgatgccctc ggcgtacaac taccagagtc
    23221 tctgccagag atttatttgc atagagtcag ttacccgctt cgcgacctct ttctgcgcta
    23281 tctccgggct catgctctgg tcacggctga acaactggct catgagttta gtctcggtat
    23341 tgccattgtc gaagagcagc ttcagcaact gcgcgaacag ggtctggtga tgaatctgca
    23401 acaagacatc tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc
    23461 cgccagagaa gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg
    23521 tcagggcgta ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg
    23581 cgtttatgag ggcgtagatg gcgtgatgcg ggtaatcgaa cagcttgccg gagtcggctt
    23641 acccgcctca ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgccaga
    23701 aatgctcgat gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaaactggg
    23761 tgaagatgac ggcctggtgg cactgcatct acaggaatat gctgcagaat cgttcactcc
    23821 cgccgaagcg gatcaggcga atcgttcggc gctgcaacaa gcgatagtcg ctgttctggc
    23881 tgacggagga gcctggtttg cacaacaaat cagccaacgg atacgcgaca aaatcggcga
    23941 atcggttgat ccctcggcac tgcaagaggc gttatgggcg ctggtctggc aaggcgtcat
    24001 caccagcgat atttgggcac cgttacgcgc cctcacccgc agcagttcca acgcacgcac
    24061 ctcaactcgc cgcagtcacc gggctcgtcg tgggcgtcct gtctatgcac aacccgtctc
    24121 gccgcgggta tcttacaaca caccaaatct ggctggacgc tggtcgttat tgcaggtgga
    24181 gccactaaac gataccgaaa ggatgctggc gctggcggaa aatatgctcg accgctacgg
    24241 catcatcagt cgtcaggcgg tgatagccga aaatatccct ggcgggtttc catcgatgca
    24301 aacgctttgt cgaagtatgg aagactccgg gcgaattatg cgaggtcgtt ttgtagaagg
    24361 tctggggggt gcgcaattcg ctgaacgtct gactattgac cgcttgcgcg atctggcgac
    24421 acaagccgcg caaacgcgcc actatacacc agtggcgctc tccgccaacg atccggctaa
    24481 tgtgtgggga aatcttctgc cctggcctac acatccggca acgctggtac caacgcgtcg
    24541 ggcgggtgcg ctggtggtcg tttctggcgg caaattgtta ctctatctgg cgcaaggtgg
    24601 caaaaaaatg ctggtctggc aggaaaaaga ggaattactc gccccagagg ttttccacgc
    24661 gctgactacc gcactgcgtc gcgaaccacg gctgcgcttt acgctaacag aagtgaatga
    24721 tctaccggtc cggcaaacgc cgatgtttac gctgctgcgt gaagcgggat tttcaagttc
    24781 gccacaaggg ctggattggg gatagagaaa ggactgacgg atgcccgttc gcatccgtca
    24841 gtattgcagg acggattatt ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc
    24901 tttgatcagc tgctgcagtt cgccacgctg atacatttcg atcacgatat cacaaccgcc
    24961 gaccagctcg ccgtcaaccc acagttgcgg gaaggtcggc cagttagcat atttcggcag
    25021 ttccgcacga atgtccggat tctgcagaat atcaacatag gcaaaacgtt cgccacatgc
    25081 ggcaagcgcc tggactgcct gggcagagaa accgcagctc ggcagtttcg gtgaaccttt
    25141 catgtacagc aggatcgggt tttcagcaat ctggcgttgg attttttcga tagtggtgct
    25201 cattgtcttg cttcctcaaa cttctttacg gcagtaatct gacattgtag cgggtcagtg
    25261 cggcatcgga aaataacatt ttcatcacgc tttagctatt ttatcccttt gctctatttg
    25321 ttgcatttca aatattcgtt tcttcccaga tgcacgacaa actggcgtat ttataaccat
    25381 ttttagcttt cactgctgca tttttttgcg ctcgccaacg aaacgtattt tttaacaata
    25441 aaagctatta actttctctt cttctatgca ttagaatcat caagttttgt aaatcagacg
    25501 caggcatgat agacctgcct taacagaggg acgctcagtg gcgcggataa accgtatttc
    25561 gatcacgctc tgtgctttac tttttaccac cctgccttta acgcctatgg cccatgcttc
    25621 aaagcaagcc agggagagtt ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa
    25681 aagcacggca accaccaaaa agacccagaa agccagccag aaaacagcga aaaaagccgc
    25741 cagtaaaagt acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc
    25801 ttctaaaaac gccaaaactc gcagcaaact cgccgtcaat aaaacggcct cagccagctt
    25861 taccgaaaag tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc
    25921 cgcgtcagga acccttgccg acgcacacaa agcgaaggtg caaaaagcca caaaagtggc
    25981 aatgaataaa ctgatgcagc aaattggcaa gccatatcgt tggggtggca gctcaccgcg
    26041 taccggtttt gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg
    26101 aattccgcgt acggcgaatg aaatgtatca cctgcgtgat gcagcaccta tcgaacgtag
    26161 tgaactgaaa aacggcgacc tggtcttttt ccgtactcag ggacgcggta cagccgatca
    26221 tgtcggtgtg tatgtcggca acggcaaatt tattcagtca ccgcgtacgg gtcaggaaat
    26281 tcaaatcact tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgccgggt
    26341 aatgacccca aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt
    26401 catcttttgt ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta
    26461 cgtatgcaaa ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat
    26521 atgctaaaga tgctctggca ccgcatattt ctgcggaaac tatcgagtat cactacggca
    26581 agcatcatca gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag
    26641 gtaaatcact ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc
    26701 aggtctggaa ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga
    26761 ctggaaaagt cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagctc
    26821 agtttactga tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca
    26881 gcgatggcaa actggctatc gtttcaacct ctaacgcggg tactccactg accaccgatg
    26941 cgactccgct gctgaccgtt gatgtctggg aacacgcata ttacatcgat tatcgcaatg
    27001 cacgtcctgg ttatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa
    27061 atctcgctgc ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat
    27121 gccaacgcgg atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa
    27181 ctgtaacgca gaaggttatc cttctgcgtt tttgtttaat tagctgttag caacgcaaac
    27241 tgtttcaggt tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat
    27301 cgcccccatc accggcacaa agctgtatcc cagcccagcg gaaattaccg cgccaccagc
    27361 agctgctccc agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc
    27421 tggcgcttca ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt
    27481 tgctgcgccc cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg
    27541 gattgccagc ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga
    27601 acgatctgcc agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac
    27661 cagcattgcc gtgacgaaca ctggtgttgc gtgggtaata ctttgcagta ccggagagat
    27721 ataggtgtag agagtaaaca ttgcaccagc tcccagtacc gtcgtcagca atgcagacag
    27781 cacctgtgga cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgcacc
    27841 acctttaggt aatgagaaga acagacttac cattgaaatc acgcccagcc ccgccgttgc
    27901 cagaaatgac atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc
    27961 gatatttgcc agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt
    28021 tggcaccacg cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct
    28081 ggtcaaaatg cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt
    28141 gcctagcgtg aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag
    28201 tagaagcgtc atcagcggcg cgccaaccat tacgccaact gcataggcac tgattaacat
    28261 tccggcagcg ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg
    28321 cgagaactcc gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt
    28381 aattttcatg ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga
    28441 aagcatgaca gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt
    28501 ttgctaacaa acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg
    28561 cagcgcggca gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag
    28621 tgaaaattta aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg
    28681 atattacgca tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg
    28741 tttttaccac ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg
    28801 tactggccat tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt
    28861 ttgttgtgcg taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag
    28921 ttagggtctg gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt
    28981 ttccactaca actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg
    29041 caacgccgtg tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag
    29101 cctgaaggtt aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggctta
    29161 ttttgccgag atcattgaag cagttgaaaa aaattgcttc cagaaaggtt acaccctgat
    29221 tctgggcaat gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca
    29281 aaaacgcgtc gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat
    29341 gctggaagag tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga
    29401 cttcaccgat gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct
    29461 gattgaacgc ggtcaccgcg aaatcggcgt tatacccggc ccgctggaac gtaacactgg
    29521 cgcaggccgc cttgccggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga
    29581 aagctggatt gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat
    29641 cctgtcgcag ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg
    29701 cgcactttgt gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctgatcgg
    29761 ttatgataac gtgcgcaacg cccgctattt tacgccggcg ctgaccacga tccatcagcc
    29821 aaaagattcg ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg
    29881 tgaagaaccg cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga
    29941 cggcccgttc cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg
    30001 cagccactcc ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc
    30061 aggcgacata tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc
    30121 cagttctaat gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac
    30181 catccctatg cataatcctg cagagataca ggttgccgat gattcccagt caggtacgac
    30241 aagtcttttt tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc
    30301 ttcgcgcacc aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa
    30361 cgccagcggg tggtgactgg caacaacgca actccagctt agcatcccca tatcccggaa
    30421 ggcataacga ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc
    30481 gtcggaaagc gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc
    30541 atcaaaatgg cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt
    30601 atccactgcg atagctaact gaccgcgcca gccgttcgct atctgctgac attgctgccg
    30661 agtgatctgc atttttttga caacagagcg cccttctttg agaaaccacg ctccggcagc
    30721 ggtcagctcc acatcacggt gccgtcgttc aaacagcggc accgccagcc actcttccag
    30781 ctgacgtacg gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct
    30841 aaaactacca ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt
    30901 ctgcctgcaa aatttttgaa accagtcatc aaatattacc gtttcacaac actaatttca
    30961 ctccctacac tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg
    31021 gaaaagattt ttagtctggc tggcgggttt gagcatactc ggttttctgg caaccgatat
    31081 gtatctgcct gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag
    31141 tgccagcctt agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct
    31201 ctccgaccgt tatggtcgta aaccggtatt attaatcggc ctgacaattt ttgcgttagg
    31261 tagtctgggg atgctgtggg tagaaaacgc cgctatgctg ctggtattgc gttttgtaca
    31321 ggctgtgggt gtctgcgccg cggcggttat ctggcaagcg ttagtaacgg attattatcc
    31381 ttcacagaaa gttaaccgaa tttttgcgac catcatgccg ctggtgggtc tgtctccggc
    31441 cctggctcct ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc
    31501 caccctgttt gccattaccg ttgtgctgat tctgcctatt ttctggctca aacccacgac
    31561 gaaggcccgt aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac
    31621 ctatcgcggc aacgtgctga tatacgcagc ctgttcagcc agtttttttg catggctgac
    31681 cggttcaccg ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta
    31741 tgtcccgcaa actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa
    31801 atggcaaggc aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc
    31861 gacctgggct gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg
    31921 tgtgatggcg attgctaatg gcgcgatcta cccgattgtt gtcgcccagg cgctgcgtcc
    31981 cttcccacac gcaactggtc gcgccgcagc gttgcagaac actcttcaac tgggtctgtg
    32041 cttcctcgca agtctggtag tttcctggct gatcagtatc agcacgccat tgctcaccac
    32101 caccagcgtg atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg
    32161 taaagaagtt ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg
    32221 atctatatcg atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt
    32281 agcggcctat actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga
    32341 caggatcgtt cccgactcac tatggatggt catttcggca agggttcttc ctttccctct
    32401 gttctacgtc ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa
    32461 agcgtctacg ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca
    32521 tcgtgtatag aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta
    32581 cttagccgtg ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac
    32641 cccgattttt ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg
    32701 gatggctggt gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt
    32761 ctcgagaacc aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc
    32821 ttcaatctgc agagtaaaaa acgtgcctgg atagtcggca aagagcatta cgatttgggt
    32881 aatgacttgt tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa
    32941 gatgccgata atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg
    33001 cagttaaaac cagggatgcg cgtactggat attggctgcg gctggggcgg actggcgcac
    33061 tacatggcgt ctaattatga cgtaagcgtg gttggcgtca ccatttctgc cgaacagcaa
    33121 aaaatggctc aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt
    33181 gacctgaacg accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg
    33241 aaaaattacg atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc
    33301 ctgctccata ccatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa
    33361 tatattttcc cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc
    33421 cactttgtga tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg
    33481 tggtatgaac gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt
    33541 aaacgaatgt ttacctatta tctgaatgcc tgtgcaggtg ctttccgcgc ccgtgatatt
    33601 cagctctggc aggtggtgtt ctcacgcggt gttgaaaacg gccttcgagt ggctcgctaa
    33661 aggctattct atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg
    33721 attcatggca ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt
    33781 ttgtggatcg atttcaatgt tgacgcgtgc gccaagtttt ttcttcccaa gagtcgtgcg
    33841 ttccagtgtt tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccggcggt
    33901 caggctaata ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact
    33961 atcctggact ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc
    34021 agcggtggtc ataatatgac ctgacattaa gtgtccgcca atttcatcac tgaatttcgc
    34081 cgcacgctca acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgcaa
    34141 cgtttctttc atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca
    34201 gcaaccgtta tgcgccacgg acgcaccggt ttccaggccg tccagcatgt ggtcgggtaa
    34261 ctccaccaca tgcgtgcgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc
    34321 ctgtacaatc cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag
    34381 cacaatagca ggtggcaaac gcccttacca gtgaaggggt aagaatgact attttttcac
    34441 tggagaatta ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct
    34501 gcccattcag gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca
    34561 gaagtatatc agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca
    34621 aatcgcccaa actgcgatgg gttttgtcga taccgtgatg gcgggcggct acagtgccac
    34681 cgacatggcg gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca
    34741 cggactgctg ctggcattaa cgccggttat cgcgcaatta aatgggtccg gtcgacgtga
    34801 gcgcattgcg catcaggtgc gacaaggttt ctggctggcg ggttttgttt ccgttctcat
    34861 tatgctggtg ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc
    34921 tctggcggac aaagccgtgg gttatctgcg tgcgttgctg tggggcgcgc caggctatct
    34981 tttcttccag gttgcccgta accaatgtga aggtctggca aaaaccaagc cgggtatggt
    35041 aatgggcttt atcggcctgc tggttaacat cccggtgaac tatatcttta tttatggtca
    35101 tttcggtatg cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cagtgtattg
    35161 ggtcatgttc cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatattcg
    35221 taacgaaaaa ggcaccgcga aacccgatcc tgcggttatg aaacgactga ttcaactcgg
    35281 tttgccgatt gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt
    35341 agtgtctccg ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc
    35401 actaatgttc gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg
    35461 tctgggtcag ggctcaacgc tggatgcgca aaccgctgcg cggaccggac ttatggtggg
    35521 tgtctgtatg gcaaccctga cggccatatt cacggtttca ctgcgggagc aaatcgccct
    35581 gttgtacaac gacaatcccg aggttgtaac gctggcggcg catttgatgt tgctggcggc
    35641 ggtatatcag atttctgact caatccaggt gattggcagt gggattttgc gtggttataa
    35701 agatacgcgt tccattttct atatcacctt tacggcttac tgggtgctgg gcttgccaag
    35761 cggctatatt ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg
    35821 gataggcttt attattggcc tgacgtcggc agccattatg atgatgctgc gtatgcggtt
    35881 cctgcaacgt atgccatcag ccatcattct gcaacgagca tcccgctaat aaagacaagg
    35941 cgcaaccttc acggggtgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg
    36001 tcttcgtctg gctgtagttt cagtgcgtta cacagagttt gccgactggc atctgttatc
    36061 tcaacgcgta tatccccggt aacttcccct ttcacattga tgaagtcagg tgaaatttct
    36121 ttcgcactgg tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc
    36181 acatgcgttg ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca
    36241 acaatgaaag tgacactgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc
    36301 tgctgtacag taatcgttga ttgttgcaga tctgcgtcat agccaataat cattgaagcg
    36361 ccgtccagta atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca
    36421 aaattttcgt ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca
    36481 ttttccagcg taccggcatg cacttcagcc cgaccaccca cattcacgga caggttttgc
    36541 gtcttaccgg aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc
    36601 tcaacaatag tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac
    36661 gtatcactgg ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg
    36721 gcttccccac ctggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt
    36781 gtcccattaa ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct
    36841 tccagtacac gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca
    36901 acgctgaatt caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt
    36961 gcgcctccct cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg
    37021 ccattgttac cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca
    37081 gcccccgtga ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca
    37141 gtaagttgcg tcgcgccaat gacctcgccg atcttaacaa tttgcacatt cccgtcgctc
    37201 atcaagtttt tcgcatcaga ccccatgatt tattcctttg ctgcatctgt gtgcctttat
    37261 tgctacctaa gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa
    37321 acaactctgt ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc
    37381 ctgaataaaa atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc
    37441 aaatggtcga aatagcgcag aaaattacgt tttgcctctt gccaccttcc cactctgccg
    37501 ctaatattcg tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca
    37561 ccaccttgac atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc
    37621 gtagctcagt tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact
    37681 cggacgcacc agattttctt aatctggtct tctacttttt ccctctgctt cttctctgta
    37741 tccaatacgt taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat
    37801 ggcaactttg ttacaacttc atttttcttt caatggccca tttggcgacg caatggctga
    37861 gcagcttaaa ccgcttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg
    37921 gacccaaagt gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag
    37981 cgcccttgcc tatctggaaa aacacactgc ccgactgaaa aatctcggcg ttgaggaagt
    38041 tatcgccaaa gttttcgatg tcaatgagcc actgagtcaa atcaatcagg caaaactcgc
    38101 ctgacagatt ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa
    38161 attctaaaaa tttcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg
    38221 taagtaacaa tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta
    38281 tcctcgcttc tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag
    38341 gccggtgtcg ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt
    38401 gccagtattg aaataccgcc gatttattgt acgtatctcg aatggctaca aaagcaagaa
    38461 gccagccatc tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta
    38521 ccgcgaattc tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca
    38581 cgaaagcaaa aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt
    38641 acaaatgctg gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta
    38701 gcggtgattg ccacgggtca cgtctggcct gatgaagaag aagcaatccg aacgtatttt
    38761 cccagcccgt ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg
    38821 ggaacatcct tgagcggact ggatgcggca atggcagtgg caattcagca tggttcgttc
    38881 attgaagatg ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat
    38941 attacgttaa tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc
    39001 tacgagccct tacacatcgt cactgctcag gcattaaatg ctgagattca aaaaggcgaa
    39061 tatggccttt tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca
    39121 gactggagcc aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg
    39181 tttgccgagc gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc
    39241 gaacgcaata aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat
    39301 gaagcagtac aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa
    39361 ggccttgccc gggttttcat cgataattat gccgcaatcc cttcagagtc tattcgtcgg
    39421 ctgctggcct tacgtgaagc gggaatcatt catattctcg ctctcggtga agactacaaa
    39481 atggaaatta acgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac
    39541 gtttttattg atgcccgcgg gcagcgtccg cttaaagtga aagatattcc tttccctgga
    39601 ctacgcgaac aattacagaa aacaggggat gaaatccctg atgttggtga agattatacg
    39661 ttacagcaac ccgaagatat tcgtgggcgc gtagcgttcg gcgcgttgcc ctggttaatg
    39721 cacgaccagc ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct
    39781 cgggcggtcg taaaacctgc atcccttgct cgtcggcgtc tttcgtttga ttaaaggtaa
    39841 agctatcagc actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggctcga
    39901 atattgtttt caccttcaat ggcgacggcg aaacgaactc tttcgccgtc atgatatgcc
    39961 agtgcggcgc ttcctcctgg cccgagaata attgagtcga ccacgcccgt gctcactacc
    40021 gttgaatttt caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg
    40081 tttgcgccaa agctggcgat ttgcaccagg tcaccattac tagcgacatg acatcgttca
    40141 cccaatgtac aaacgcgtac tcgcattccg gtattcgcaa tccgactgct atctccagcg
    40201 ctgcttatcc gcgaagaatt gccggttaca gcaacccgtg cgcgctcacc ggagctgcca
    40261 atgtgactgt taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgt
    40321 gcagcatacc cagcggttgc gattctggcg ttatcctcac aacagccgat gcgagcatga
    40381 tcgtcgctgc gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt
    40441 tgcatagagg aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca
    40501 tactcaacca gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata
    40561 tcggcataac taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg
    40621 atacgccatt ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca
    40681 tcgaccatgc ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatga
    40741 ccgaagataa aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat
    40801 cttacgccag gaaacacatc tcccacggct tgcggataga gacacagcag ccccgtcaat
    40861 agtaacaacg gcagcaatcc atacatgaca ccaacatagg caacctgctg taagggatta
    40921 aatttagact gggttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa
    40981 taaaatcgcg tttgttttgc cgcccgctcc agccaaccct gacgacgaat gcgatagtgg
    41041 tggccattac ccccaacggc attgatcaga acaaagccga gccagcatgc cagtaacaaa
    41101 aatccgcaaa cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccagcgca
    41161 aagtgattta tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc
    41221 cataatctga ccgctttgct gtaaagataa atcctctcac catgatcggt ggctgatttc
    41281 ttcacgccac gagcacgaag caaggcgtgc tgccccagca ccaaccacat cccaaccagc
    41341 aatactccgg caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag
    41401 ttcgctaact ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt
    41461 taatcaaatg ttgaccgaac cggcgataca gatgcggttt cccggcacca ggtagctgat
    41521 attgatagta tttattatcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa
    41581 agatcaatgc gtgttccggg caggcgctga cgcaaatggg cggaaagcct tttgccagtc
    41641 gggactcagc gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa
    41701 cctggtacgg acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc
    41761 gcacgatccc ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag
    41821 catcttcgca gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat
    41881 cggtgactgg aatgtgtgct atcgataaac ggcttccctg agcggggacg tgattggttt
    41941 tacggcatgc gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg
    42001 cataccgaac ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa
    42061 aaaagattac tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat
    42121 cccctaatgc agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg
    42181 ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca
    42241 taaatcgctc gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg
    42301 catattcagg tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt
    42361 aatatggcgc ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa
    42421 aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg
    42481 ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta atcatcctgg cttcccgtca
    42541 tacacgcgat atccgcgtag tctccgttct gcttgatgtt ttccagcaaa actgacagtt
    42601 cctcttctgc aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag
    42661 ccgcagaatg ctggcgaatg aactctgcca ggatttcagc cggttctggt tccgttaccg
    42721 tatcaacggg gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga
    42781 ctttcgctaa cggcaattca tcccgatggt tcatcttacc cattccttac gcaggcagta
    42841 gattgtgtgc tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc
    42901 gggttgggca accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag
    42961 catgcatatc gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat
    43021 ccttgtcgaa tacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca
    43081 tcagtttcac cgtgtaggca cgatgcaatg taaagatacg ctctgcagct aaatctaatt
    43141 tttcctgagt catctcttcg ccagtgatcg ctttgaagaa tttggcttca agcgccagat
    43201 cgccccgata attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca
    43261 gggtgacggc gttatgcaaa cagacccgca acagcgacca cttagcatat ttaatttttg
    43321 cgtcattgat cggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagct
    43381 ctttcgccac ttcacgctgc agtttcaatg gcaaacccga accaataaaa ttgatatggg
    43441 tatgcgtcat gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg
    43501 cttcattggc atggtgaagc ggatagccaa acggcgacca gagtttattt ttcgcgtagc
    43561 cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt
    43621 ggctcagctc acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt
    43681 cacccgcttc cagttgatcc cagcgaattt ccgcatactc ttcagctggc agaacacgct
    43741 tgaacacccc tttgctgtaa cagtaggtaa agtcgcgatg cagctgcccg tagttacacc
    43801 ataggccgta gtcatcgaac aaattcagac caacaaggtt accaatcaca cgaccatcgt
    43861 ctttatcttc aaaatctttc ggcccgttcg ggaagatggt ggtatggaca aagtttgcta
    43921 cacaagtgtt accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt
    43981 gggtcataca acggatcggg caagagtggc agccgctcat ttttactgtg tatttctctg
    44041 ccgccggtcc taagtcaaaa acggatttat aggtacgaaa gccgaccgta ttctgattgc
    44101 ctggcggaat ttcacccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt
    44161 tacgtgctgt ccagcgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg
    44221 gcacgacatg gttgttattc gcaccgataa gttcagtcat catgtaatcg ttgagacgct
    44281 tcatctcctg acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca
    44341 ggtttttcga acccattatt gcgccagttc ccgcaccacc gctgtggtta cggctattca
    44401 acatgccaga gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt
    44461 ccggactggt caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga
    44521 aatctgcttt ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg
    44581 ccttcccttc gataataatg acgtcgtagc cagcgaattt catttgcgcc gcaaaaaagc
    44641 cacccatatg ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat
    44701 ttacgcgaga actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt
    44761 tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc
    44821 cgaagcccat gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc
    44881 ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg attttttcct
    44941 ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca
    45001 ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac
    45061 ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac aacgtttatg
    45121 atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt
    45181 catgcattgc ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata
    45241 caggccgccg ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat
    45301 acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg
    45361 gtgacaaccc gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa
    45421 ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg
    45481 ggcaattgtt agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa
    45541 gccaatatct aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac
    45601 acagtgctga caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg
    45661 tccccaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat
    45721 aaactaatcc tttatggtta ggagaaaaaa taatgtggtt attctgg
//
LOCUS       DAZUGK010000013        45634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZUGK010000013 DAZUGK010000000
VERSION     DAZUGK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.013"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            195..1766
                     /gene="garD"
                     /locus_tag="ACHY5W_000637"
     CDS             195..1766
                     /gene="garD"
                     /locus_tag="ACHY5W_000637"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFS4445596.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKTGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1914..2249
                     /gene="prlF"
                     /locus_tag="ACHY5W_000638"
     CDS             1914..2249
                     /gene="prlF"
                     /locus_tag="ACHY5W_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFS4445597.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDNIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2249..2713
                     /gene="yhaV"
                     /locus_tag="ACHY5W_000639"
                     /pseudo
     CDS             2249..2713
                     /gene="yhaV"
                     /locus_tag="ACHY5W_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
     gene            complement(2768..3577)
                     /gene="agaR"
                     /locus_tag="ACHY5W_000640"
     CDS             complement(2768..3577)
                     /gene="agaR"
                     /locus_tag="ACHY5W_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFS4445598.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3826..5106
                     /gene="kbaZ"
                     /locus_tag="ACHY5W_000641"
     CDS             3826..5106
                     /gene="kbaZ"
                     /locus_tag="ACHY5W_000641"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFS4445599.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFAEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGIEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5129..5602
                     /gene="agaV"
                     /locus_tag="ACHY5W_000642"
     CDS             5129..5602
                     /gene="agaV"
                     /locus_tag="ACHY5W_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFS4445600.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLALVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5613..6392
                     /gene="agaW"
                     /locus_tag="ACHY5W_000643"
     CDS             5613..6392
                     /gene="agaW"
                     /locus_tag="ACHY5W_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFS4445601.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6382..7260
                     /gene="agaE"
                     /locus_tag="ACHY5W_000644"
     CDS             6382..7260
                     /gene="agaE"
                     /locus_tag="ACHY5W_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFS4445602.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGASLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEIIAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7278..7712
                     /gene="agaF"
                     /locus_tag="ACHY5W_000645"
     CDS             7278..7712
                     /gene="agaF"
                     /locus_tag="ACHY5W_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFS4445603.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7709..8842
                     /gene="nagA"
                     /locus_tag="ACHY5W_000646"
     CDS             7709..8842
                     /gene="nagA"
                     /locus_tag="ACHY5W_000646"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4445604.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKCAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9193..10347
                     /gene="agaS"
                     /locus_tag="ACHY5W_000647"
     CDS             9193..10347
                     /gene="agaS"
                     /locus_tag="ACHY5W_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFS4445605.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     NPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPPGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10359..11216
                     /gene="kbaY"
                     /locus_tag="ACHY5W_000648"
     CDS             10359..11216
                     /gene="kbaY"
                     /locus_tag="ACHY5W_000648"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFS4445606.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSYKRLR"
     gene            11354..12925
                     /gene="malX"
                     /locus_tag="ACHY5W_000649"
     CDS             11354..12925
                     /gene="malX"
                     /locus_tag="ACHY5W_000649"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/glucose-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFS4445607.1"
                     /translation="MTQEKSIKSRVWEFFQSLGKTFMFPVSLLAFMGLLLGIGSSVTS
                     PSTINNFPFLGGELTQLTFGFIAMVGGFAFTYLPVMFAIAIPLGMAKRNKAVGAYSGF
                     VGYMLMNMSINYYLTVTHQLADSATMKQVGQSVVLGIQTLEMGVLGGIIAGVITYFLH
                     ERFQDTVLHDAFAFFSGIRFVPIITSLTLSLVGLAIPLLWTYVAMGIAGIGHIIQSTS
                     VFGPFLYGVGVLLLKPFGLHHILLAMVRFTPAGGTEIVDGHEVAGALNIFYAELKAGL
                     PFSPHVTAFLSQGFMPTFMFGLPAVAYAIYRTAKPENRPVIKGLLLSGVLVSVVTGIS
                     EPIEFLFLFIAPALYIFHIIMSGLALMVMAILGVTIGNTDGGILDLLIFGIMQGTSTK
                     WYLILPVGIVWFAVYFFVFRWYILKHDIKTPGREDSSDDAAQAVAANSKARGKSKYDH
                     AVILSALGGKENIDSLDNCITRLRLVVKDMSKVDQATLKNAGALSVVVLDAHSVQVII
                     GPQVQSVKTGIEALI"
     gene            12938..14110
                     /locus_tag="ACHY5W_000650"
     CDS             12938..14110
                     /locus_tag="ACHY5W_000650"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4445608.1"
                     /translation="MFDFDKIIERKSDKCRKWDHQFVCSRFGAVAEDFIPLWIADMDF
                     TSPPAVIDGFQRIVEHGTFGYTYAFNEFYEAVIGFQRDRHHVSIEQDWITLTYGTVST
                     LHYMVQAFCQPGDSVMMNTPVYDPFAMATERQGVKVLANPLSIADSRYQLDFSLVEDQ
                     LKLHKPKLWFFCSPHNPSGRIWTADEIRQVSDLCKRYGVVLVVDEVHAEHLLEGEFVS
                     CLASGCADHDNLVLLTSPNKAFNLGGLKTSYSVIPNERLRKRFHQRLEVNSITSPNIF
                     GVWGITLAYQHARPWLDALNIYLRNNSHWLNDALKTHFPEWKMMTPESSYLAWVDVSE
                     SGRSATEWVNHFARNAGVIIEDGSHYVQNGENYLRFNFGTQRYWLEQALERMQKIA"
     gene            14202..14552
                     /locus_tag="ACHY5W_000651"
     CDS             14202..14552
                     /locus_tag="ACHY5W_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4445609.1"
                     /translation="MTLPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVASDN
                     IQQVLFNLASPYFLFTFLRFQTHSENISQPCIRARNFSSNQRSASSLLRRLFQSRESV
                     KAGTSSGTSANCNP"
     gene            complement(14438..15337)
                     /locus_tag="ACHY5W_000652"
                     /pseudo
     CDS             complement(14438..15337)
                     /locus_tag="ACHY5W_000652"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15419..16375
                     /locus_tag="ACHY5W_000653"
     CDS             15419..16375
                     /locus_tag="ACHY5W_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4445610.1"
                     /translation="MKLLLTLVTSLVLSVNALATPIPVPSPDTQAPGYYRMILGKLRI
                     TAVSDGTVTIPLDKLLTHISADKLRQQLAKDSLTPHTETSINAFVIDNGQQRVLVDAG
                     AGDLFGNNGGQLLENLAAAGYPAETIDAVLLTHIHADHSGGISRQGKPAFPNAQVFVE
                     QKDADFWLNPANASKVEDSQKHTFVESEQTIRPVINAGQLKTFIAPATVFADITATST
                     AGHTPGSVMYTVESEGQTLVLWGDIIHAKAVQMPDPHVAIHFDVDQQQAVKTRERVLK
                     HAAQGGYWVAAAHISFPGLGHVKSDSKGYRWVAANYTTQLAP"
     gene            complement(16485..17348)
                     /gene="rsmI"
                     /locus_tag="ACHY5W_000654"
     CDS             complement(16485..17348)
                     /gene="rsmI"
                     /locus_tag="ACHY5W_000654"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4445611.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            17413..19449
                     /gene="lpoA"
                     /locus_tag="ACHY5W_000655"
     CDS             17413..19449
                     /gene="lpoA"
                     /locus_tag="ACHY5W_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFS4445612.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            19407..19802
                     /gene="yraN"
                     /locus_tag="ACHY5W_000656"
     CDS             19407..19802
                     /gene="yraN"
                     /locus_tag="ACHY5W_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFS4445613.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            19822..20412
                     /gene="diaA"
                     /locus_tag="ACHY5W_000657"
     CDS             19822..20412
                     /gene="diaA"
                     /locus_tag="ACHY5W_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFS4445614.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            20422..20997
                     /gene="dolP"
                     /locus_tag="ACHY5W_000658"
     CDS             20422..20997
                     /gene="dolP"
                     /locus_tag="ACHY5W_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS4445615.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNSELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(21111..22151)
                     /gene="yraQ"
                     /locus_tag="ACHY5W_000659"
     CDS             complement(21111..22151)
                     /gene="yraQ"
                     /locus_tag="ACHY5W_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4445616.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAVGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(22224..22859)
                     /gene="yraR"
                     /locus_tag="ACHY5W_000660"
     CDS             complement(22224..22859)
                     /gene="yraR"
                     /locus_tag="ACHY5W_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS4445617.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            22987..23505
                     /gene="yhbO"
                     /locus_tag="ACHY5W_000661"
     CDS             22987..23505
                     /gene="yhbO"
                     /locus_tag="ACHY5W_000661"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFS4445618.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(23485..23928)
                     /gene="yhbP"
                     /locus_tag="ACHY5W_000662"
     CDS             complement(23485..23928)
                     /gene="yhbP"
                     /locus_tag="ACHY5W_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFS4445619.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            23979..24281
                     /gene="yhbQ"
                     /locus_tag="ACHY5W_000663"
     CDS             23979..24281
                     /gene="yhbQ"
                     /locus_tag="ACHY5W_000663"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFS4445620.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(24268..24771)
                     /gene="yhbS"
                     /locus_tag="ACHY5W_000664"
     CDS             complement(24268..24771)
                     /gene="yhbS"
                     /locus_tag="ACHY5W_000664"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4445621.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(24765..25289)
                     /gene="ubiT"
                     /locus_tag="ACHY5W_000665"
     CDS             complement(24765..25289)
                     /gene="ubiT"
                     /locus_tag="ACHY5W_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFS4445622.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            25498..26493
                     /gene="ubiU"
                     /locus_tag="ACHY5W_000666"
     CDS             25498..26493
                     /gene="ubiU"
                     /locus_tag="ACHY5W_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4445623.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            26502..27380
                     /gene="ubiV"
                     /locus_tag="ACHY5W_000667"
     CDS             26502..27380
                     /gene="ubiV"
                     /locus_tag="ACHY5W_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4445624.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            27461..28468
                     /gene="yhbW"
                     /locus_tag="ACHY5W_000668"
     CDS             27461..28468
                     /gene="yhbW"
                     /locus_tag="ACHY5W_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFS4445625.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAAVSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(28585..29829)
                     /gene="mtr"
                     /locus_tag="ACHY5W_000669"
     CDS             complement(28585..29829)
                     /gene="mtr"
                     /locus_tag="ACHY5W_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFS4445626.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(29983..31872)
                     /gene="deaD"
                     /locus_tag="ACHY5W_000670"
     CDS             complement(29983..31872)
                     /gene="deaD"
                     /locus_tag="ACHY5W_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFS4445627.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(31865..31945)
                     /gene="yrbN"
                     /locus_tag="ACHY5W_000671"
     CDS             complement(31865..31945)
                     /gene="yrbN"
                     /locus_tag="ACHY5W_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFS4445628.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(32052..32936)
                     /gene="nlpI"
                     /locus_tag="ACHY5W_000672"
     CDS             complement(32052..32936)
                     /gene="nlpI"
                     /locus_tag="ACHY5W_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFS4445629.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGMRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(33045..35180)
                     /gene="pnp"
                     /locus_tag="ACHY5W_000673"
     CDS             complement(33045..35180)
                     /gene="pnp"
                     /locus_tag="ACHY5W_000673"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4445630.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(35427..35696)
                     /gene="rpsO"
                     /locus_tag="ACHY5W_000674"
     CDS             complement(35427..35696)
                     /gene="rpsO"
                     /locus_tag="ACHY5W_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4445631.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(35845..36789)
                     /gene="truB"
                     /locus_tag="ACHY5W_000675"
     CDS             complement(35845..36789)
                     /gene="truB"
                     /locus_tag="ACHY5W_000675"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4445632.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPIERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(36789..37190)
                     /gene="rbfA"
                     /locus_tag="ACHY5W_000676"
     CDS             complement(36789..37190)
                     /gene="rbfA"
                     /locus_tag="ACHY5W_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4445633.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(37354..40026)
                     /gene="infB"
                     /locus_tag="ACHY5W_000677"
     CDS             complement(37354..40026)
                     /gene="infB"
                     /locus_tag="ACHY5W_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4445634.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(40051..41538)
                     /gene="nusA"
                     /locus_tag="ACHY5W_000678"
     CDS             complement(40051..41538)
                     /gene="nusA"
                     /locus_tag="ACHY5W_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4445635.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(41566..42018)
                     /gene="rimP"
                     /locus_tag="ACHY5W_000679"
     CDS             complement(41566..42018)
                     /gene="rimP"
                     /locus_tag="ACHY5W_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4445636.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(42225..42301)
                     /locus_tag="ACHY5W_000680"
     tRNA            complement(42225..42301)
                     /locus_tag="ACHY5W_000680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42265..42267),aa:Met,seq:cat)
     gene            42649..43992
                     /gene="argG"
                     /locus_tag="ACHY5W_000681"
     CDS             42649..43992
                     /gene="argG"
                     /locus_tag="ACHY5W_000681"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFS4445637.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(44000..45624)
                     /gene="yhbX"
                     /locus_tag="ACHY5W_000682"
                     /pseudo
     CDS             complement(44000..45624)
                     /gene="yhbX"
                     /locus_tag="ACHY5W_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT        11075 a        12104 c        11470 g        10985 t
ORIGIN      
        1 acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat
       61 ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca
      121 cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg
      181 gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata
      241 taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaaacag
      301 gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg
      361 cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg
      421 ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag
      481 cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggaaccctta ccgccgctgg
      541 aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc
      601 ttggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactacgta gtaaaaatca
      661 ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt
      721 tgtacggttg tggcgtggcg attaacgcac cggcggcagt tgtacctatc cgtaccattc
      781 acaatatttc gctgaatcct aactttggcg gcgaagtaat ggtgattggc ctgggttgtg
      841 aaaagttgca gcctgagcgc ctgctgactg gaacggatga tgtgcaagct attccagtag
      901 aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg
      961 aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa
     1021 cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg
     1081 gcgtaacggc aaacccggcg gttgggtatg cgtctgatct actggtgcgc tgcggcgcaa
     1141 cggtgatgtt ttcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg
     1201 cagtgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc
     1261 tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc
     1321 tggcaaacgt ggtagagaaa gcgctcggct ccattgctaa atcgggtaaa agtgcaattg
     1381 ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc
     1441 cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcaca gtgcaagtgt
     1501 ttacgaccgg ccgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcaa
     1561 cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg
     1621 ccaccggcga agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg
     1681 tcgccagcgg gaagaagaaa accttctcgg atcaatgggg actgcataac cagctggcgg
     1741 tgtttaaccc ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct
     1801 tagtgccatg tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattact
     1861 ttgctgaagc ttataatttg agcagaaaac aggctgtaaa agtacagtgc ctaatgcccg
     1921 ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa
     1981 caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggcctg gacaacattc
     2041 attacgaaat tctgcctggt gggcaagtat ttatgtgccg actgggagat gaacaggagg
     2101 atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa
     2161 aaactcgtcc attcgacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca
     2221 ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc
     2281 gctatatgct catccctgtt ttcaggaaac ctacgacgct ttagttgccg aagtcgagac
     2341 attaaaggga aaagatcctg aaaattatta gagaaatgcc gccacaaagt tattggcagc
     2401 agttcacaaa gtgattgagg agcatatcac ggtcaatcca tcatcgccgg cattccgtca
     2461 tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg
     2521 tcgttatcgt ctcttctttc gttatagtga aaaagagaaa gtcatcattc tgggatggat
     2581 gaacgatgaa aacactcttc gcacctacgg taaaaaaaca gatgcctata ccgtattcag
     2641 caaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga
     2701 agaaaaccat tgatggtgtt cacaatgccc gccgaacgca ttcgtgttga ccagcgggca
     2761 ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccaggc
     2821 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     2881 tatgtaaact ggaacgattg aatttactgg aatcggtgac cacgatgatc cgttccgcaa
     2941 cttcgcacat ccggcggttt aaacgggctt catcttcatt atgtgtgctg acgccgcgct
     3001 ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     3061 gctcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca
     3121 gcagctcaac gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg
     3181 caattacgtc agtgtgcttg cgcatcagac gagcaatctc aaaagtggtg gtcccagaat
     3241 cgaggatcac ccgatgacct ggctgaatca actcaacggc agctttcgca acgctgcgtt
     3301 tcattgcggt gttcagcgcg cttttatctt ccactgatgg ctcgactgac ggcgttgtgc
     3361 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     3421 ccagatcgtt gcggatcgtc acagtagata cgccatacaa tgccgacaga tcgttaacct
     3481 gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg
     3541 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     3601 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc
     3661 ccattggcgc aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga
     3721 ttagaaaact attatctttc gttttatttt tatctcacca tgacgcagta tcaattgaaa
     3781 caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca
     3841 gaaatggtga gacagcacaa agcgggcaaa acaaatggaa tttatgccgt ttgttccgca
     3901 catccgctgg tgctggaggc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     3961 attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc
     4021 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg
     4081 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccggc cgctcaggca
     4141 atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac
     4201 cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga catcgtggct
     4261 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttgccgaa
     4321 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     4381 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
     4441 cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc cctggtggtt
     4501 caacccggca tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgacc
     4561 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     4621 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     4681 ggcccggcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcagc gattgaagaa
     4741 gaactggtgc cagcaaaagc ctgttctggt ctgcgtcagg tgctggaaaa cgtgatgctc
     4801 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     4861 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     4921 gctttcgctc atctggtacg taatctggcg gattcaccaa ttccgctgcc gctgatcagc
     4981 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     5041 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
     5101 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga
     5161 acgcttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt
     5221 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
     5281 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca
     5341 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctggc
     5401 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
     5461 tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa
     5521 tgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt
     5581 ggacctcttt aaattacttt gagggattca tcatggaaat cagtctgttg caggcattcg
     5641 cgttgggcat tatcgctttt atcgctggcc tggatatgtt taacggctta acccatatgc
     5701 accgcccagt ggtcctcggc ccgttagtcg ggctggtact tggcgatctg cataccggta
     5761 ttttaaccgg cggtacgctg gaactagtgt ggatggggct ggccccgctg gcgggcgcac
     5821 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
     5881 tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
     5941 ttaccttcct gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga
     6001 acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt
     6061 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
     6121 ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag
     6181 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
     6241 tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgccg
     6301 cgctggcgat ggcgctgatc gatctgctgc gtaaatctcc tgaaccgact caacctgcgg
     6361 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa
     6421 aacgaagaaa cattgctgac tggcgtcaat gaaaacgtat atgaagatca gagcattggc
     6481 gcggagctga cgaaaaaaga tatcaatcgc gtcgcctggc gttccatgct gttacaagcg
     6541 tcttttaact acgaacgtat gcaggcttcc ggctggttgt acggcctgct gcctgctctg
     6601 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
     6661 ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt
     6721 tctaagcagg acgttaacag cattcagagc accaaaattg ccgtcggtgc gtcgcttggc
     6781 gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg gattggagcc
     6841 agtctggcat tgcaaggctc cattcttggc gcagtcgtct ttattgtgct gttcaacgtg
     6901 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
     6961 attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg
     7021 acggtaatcg gtgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcata
     7081 gcgggcgacg cagtggttaa gttacaggct gatgttattg acaaactgat gcctgccttc
     7141 ttaccgctgg tctataccct gaccatgttc tggctggtac gtcgcggctg gagtccgcta
     7201 cgcctgattg cggttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa
     7261 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagcg
     7321 gcatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttatc gccatcgatt
     7381 ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac
     7441 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
     7501 tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttgc
     7561 aactgctact ggagatggtg ttggagcgcg aagggttaag cggtgaagaa ttccgtgtac
     7621 aggcgctgga gtgtggacat cgtgggctga ccagtctggt ggacgagtta ggccgctgcc
     7681 atgaagaatg tccggtcgag gaagggatat gacacacgtt ctgcgcgcca gaaggctgct
     7741 gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg tcatcgcagc
     7801 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccctgctta
     7861 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
     7921 actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgaccac
     7981 cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca
     8041 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
     8101 gcagaataaa ggcgcgcatc cgccggagtt gtttcgcgag cttgaaattg ccgagctgga
     8161 tcaattgatt gccgtttctc agcacacctt acgcgtggta gcgctggcac cggaaaaaga
     8221 gggggcattg caggccatcc gccatcttaa acagcaaaat gtacgagtga tgctggggca
     8281 tagcgcggcg acctggcaac aaactcgcgc cgcgtttgat gctggtgccg acggcctggt
     8341 gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg
     8401 attaacggac aagtgcgcct ggctggaact gatagccgat ggtcatcatg tacatccggc
     8461 ggcgatgtcg ctgtgttgtt gctgtgcgaa agagagaatc gtactgatca ccgacgcgat
     8521 gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggcgaagaag tgcagatgca
     8581 cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc agtacgctgt ctgttgatgc
     8641 ggcagtgcgc aatatggtcg agttgacggg cgtaacgcct gcggaagcca ttcatatggc
     8701 gtcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
     8761 caaacgcgcc agcgtcgttg cgctggatag cgggctgcat gtgcaacaaa tctggattca
     8821 gggtcaatta gcttcgtttt gatagtttgc tcctttattg ggccttcact tcccccgtaa
     8881 ggcctttctt tttctttctt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt
     8941 cttatccgac ctacgatacg ccgcatccgg caatcggtga acatagcaac gtcattacgc
     9001 aattctttaa tcttcccttg tcttttcttt caatgatcac agatttcgtt ttatttcctt
     9061 tttctccatt gaactttcgg tttcttttct atagatttta atcaacgaaa gacatcacca
     9121 agtgaaatga aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact
     9181 aaggattgag ttatgccaga aaattacacc cctgctgccg ccgcaaccgg tacatggact
     9241 gaagaagaga tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg
     9301 ctacgttccg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc
     9361 atcctgaccg gagccggaac ctcagcattt atcggtgaca tcatcgcacc gtggctcgcc
     9421 agccataccg gtaaaaactt cagcgctgta ccaaccaccg atctggtcac taatccgatg
     9481 gactacctga acccagccaa tccgctgctg ttgatctcct tcggtcgatc cggcaacagc
     9541 ccggaaagcg tcgcagccgt ggaactggca aatcaatttg taccggaatg ctatcacctg
     9601 ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg
     9661 tttgccctgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt
     9721 accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc
     9781 ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa
     9841 ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag
     9901 ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga ctgcgggtaa actggcggcc
     9961 ttctatgatt ccccgcccgg attccgtcat ggaccaaaat cgctggtcga tgacgaaacg
    10021 ctggtggtgg tatttgtctc cagccaccct tacacccgtc agtatgatct tgatctgctg
    10081 gctgaactcc gccgtgacaa ccaggcaatg cgcgtaatcg ccatcgccgc ggaaagcacc
    10141 gacatcgtcg ctgccggtcc acatatcatc ctaccgccgt cacgtcactt tatcgacgtt
    10201 gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac
    10261 atgggcaata cgccggatac cccatcagcc agtggcaccg ttaaccgcgt ggtgcaaggc
    10321 gtaatcattc atccgtggca ggcataagag gatccattat gagcattatc tccactaaat
    10381 atctgttaca ggacgcccag gccaatggct acgcggtgcc tgcctttaac attcataacg
    10441 ccgagacgat ccaggcgatc ctcgaagtgt gcagtgaaat gcgatcgccg gtgatcctcg
    10501 ccggaacgcc ggggaccttt aaacatatcg cgctggaaga gatctacgcc ctgtgtagcg
    10561 cctattccac aacctacaac atgccactgg cgctgcatct cgaccaccac gaatcgctgg
    10621 atgatattcg ccgtaaagtc cacgcaggtg tgcgcagtgc aatgatcgac ggcagccact
    10681 tcccgtttgc cgagaacgtg aagctggtga aatcggttgt tgacttctgc cactcacagg
    10741 attgcagcgt ggaagcagaa ctgggccgcc tgggcggtgt tgaagatgac atgagcgttg
    10801 acgccgaaag tgcattcctg accgatccac aagaagctaa acgctttgtc gaactgactg
    10861 gcgtcgacag cctggcggta gcgattggta cggcgcacgg cttatacagc aaaacgccga
    10921 agattgattt ccagcggctg gcggaaattc gtgaagtggt ggatgttcct ctggtgctac
    10981 atggtgccag cgatgttccg gatgaatttg tccgtcgcac tattgaactt ggcgtcacaa
    11041 aagtgaacgt tgccacagaa ttaaaaatag ccttcgccgg cgcggttaaa gcctggtttg
    11101 cggaaaatcc gcagggtaat gatcctcgtt attatatgcg cgtcggaatg gatgcgatga
    11161 aagaagttgt cagaaataaa attaatgtct gtggttccta caaaaggtta cggtaattga
    11221 aaatatgtga ataacagttt cttagcagct gtattcagac aataaaaaac aacttacctc
    11281 acaaatataa aaggtcatga ttactttttc atgaccttga aactacgctt ataaaaatca
    11341 aggagtctat taaatgacac aggaaaagtc tataaagtca cgggtctggg agtttttcca
    11401 aagtttaggg aaaacattta tgttcccagt atcattactc gcttttatgg gacttctgtt
    11461 aggtataggt agttcagtaa ccagcccctc aacaattaat aacttcccct ttttaggtgg
    11521 tgagcttacc caacttacat ttggctttat tgcaatggta ggtggcttcg catttactta
    11581 tctacctgtt atgtttgcta ttgcaattcc attaggaatg gcaaaaagaa ataaagcagt
    11641 aggcgcatat tcaggttttg ttggctatat gctaatgaac atgagtatta attattatct
    11701 cactgttacc catcaacttg ccgattccgc gacgatgaag caggttggtc aatctgtagt
    11761 attaggtatt caaaccttag agatgggcgt tttaggtggt atcatcgccg gggtaataac
    11821 ttattttctc cacgaacgtt ttcaggatac tgttttacat gatgcctttg ccttctttag
    11881 cggtatccgc ttcgtaccca tcatcacttc gctaacatta tcgttagtag gcctggccat
    11941 tcctttgctc tggacctatg ttgcaatggg tattgctggt attggccaca ttattcaaag
    12001 taccagcgta ttcggcccgt tcctttatgg cgttggtgtg cttttgctta agcctttcgg
    12061 gctacatcat atcttgctgg caatggttcg cttcacacct gcgggtggaa cagaaattgt
    12121 cgacggtcat gaagttgccg gagcacttaa tattttctat gcagaattaa aagccggtct
    12181 tccctttagc ccacatgtta ctgcattttt atcacaaggc tttatgccaa cctttatgtt
    12241 tggtttgcct gctgtggctt acgcaattta tcgtacagcc aaaccagaaa atcgtcctgt
    12301 aattaaaggt ttactgcttt caggcgtatt agtatctgtt gttactggca tttctgaacc
    12361 aattgagttt ttattcctct ttattgctcc tgcgttatat atattccata ttatcatgtc
    12421 cggtctggcc ttaatggtta tggcaatcct gggcgtgact atcggtaata ctgatggcgg
    12481 gattttagat ctattaatat ttggtatcat gcagggtact tctactaaat ggtatttaat
    12541 acttccggtg ggtattgttt ggtttgctgt ttatttcttt gttttccgtt ggtacatcct
    12601 taagcatgat attaaaaccc caggccgtga agactcatct gatgatgcag cgcaggctgt
    12661 tgcagcaaat tcaaaagcac gaggtaaatc taaatacgat catgctgtga ttttgagtgc
    12721 gcttggcgga aaagaaaata ttgattcact tgataactgt attactcgtt tacgtttggt
    12781 agtgaaagat atgtcgaaag tggatcaagc cacgttaaag aatgcaggtg ccctgtcggt
    12841 ggttgtactg gatgctcaca gtgtgcaggt aattattggc ccgcaagttc aaagtgtgaa
    12901 aacgggcatt gaagctttaa tttaacagga gttaaaaatg ttcgatttcg ataaaattat
    12961 cgagcgtaaa agcgacaagt gccgcaaatg ggatcatcag tttgtctgct cacgcttcgg
    13021 tgcggttgca gaagatttca tccctctttg gatcgctgat atggatttta cttcgccacc
    13081 agctgtaatt gacggttttc aacggattgt tgagcacgga actttcggct acacctacgc
    13141 ctttaatgag ttttatgagg ccgtgatagg cttccagcgc gatcgtcacc acgtaagtat
    13201 cgagcaggat tggatcactc taacctatgg tactgtctca acgctgcact atatggttca
    13261 ggcgttctgc cagccgggcg atagcgtcat gatgaatacg ccagtctatg acccttttgc
    13321 catggcaaca gagcgtcagg gggttaaagt gctggcaaat ccgttaagca ttgcggatag
    13381 ccgttatcaa ctggattttt cgctggttga agatcagctt aaacttcata aacctaaact
    13441 atggtttttc tgctctccac ataacccttc cgggcgaatc tggacggcag acgagattcg
    13501 ccaggtttcg gacttatgta aacgttacgg cgtagttctg gtcgtggatg aagtccatgc
    13561 cgagcatctg cttgaaggtg agtttgtcag ttgcctggct tccggatgtg ccgatcacga
    13621 taatctggtc ctgttaacct cgcctaataa agcgtttaac ctcggtggat tgaaaacctc
    13681 ttattctgtc attccaaacg aaagattgag gaagcgtttt caccagcgac tggaagtgaa
    13741 ctcaatcact tcgccgaata tttttggcgt atggggaatt acgctggctt atcagcacgc
    13801 aagaccgtgg ctggatgcat tgaatatcta tttacgcaat aactcccact ggcttaatga
    13861 tgccctcaag acgcattttc ctgaatggaa aatgatgaca cccgaatcgt catatctggc
    13921 atgggttgat gtcagcgaaa gtggacgctc cgcaaccgaa tgggttaatc attttgcccg
    13981 caacgctgga gtcattattg aagatggcag ccattatgtg caaaacggtg aaaactactt
    14041 acgttttaac tttggcaccc aaagatactg gcttgaacaa gcattagaaa ggatgcaaaa
    14101 aatcgcataa accacagggc agataattaa ttatctgcct tttttattgc cgccaaaaga
    14161 acgaaaaatg aaagaaaaca aggataacag gagataaaac tatgacatta cccaatattc
    14221 tcttaacccg tattgataac cgtctggttc acggtcaggt tggcgtgacc tggacatcca
    14281 ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt cgctagcgat aatattcaac
    14341 aggtattatt taatttagca agcccttatt tcctgttcac atttttacgt tttcaaacgc
    14401 attctgaaaa catctcccag ccctgcattc gtgcgcgtaa tttctccagt aaccagcgca
    14461 gcgcctcatc attattacgc cgcttattcc agagtagaga gagcgtaaaa gcaggaacct
    14521 cgagtgggac atcggcaaat tgcaacccat aatcggctac ccagccttct gccagcccct
    14581 gcggtacggt cgctaaagcc ggtatctgtc tcaacatcaa cggtaacgat gagaaatttg
    14641 gcgtgacgta acgcacttcc cttttacgcc cgcagtcagc caaaatcaga tcgatagagc
    14701 tactgctggc accgcgataa gagaccaaaa gatgatcgat ttcagtatat acatcaaggc
    14761 ttaacggtga agtgagggat aactggagcg gatgccacat cgtgcaaaac cccatttgag
    14821 tgatcacttc tcgttccact ccggcgggca cctgctccac gacggagatt gctacgtcca
    14881 ccttatcctg ttgcactttt tcgccatcct ggaaaggatc ggataccgtg acttgtaagc
    14941 gaatcccggg tgccatttgg gctatatctg ccatcattcc aggcatcagc cagcgttcga
    15001 cccagtcact catccccagg cgaaacgtat gctgatcctg ttctggggta aaaactggcc
    15061 tttgaaaaat cacctgctgc atttgatcca tcacaggcag tagtgccgta accagatctt
    15121 gtgcacgtgg tgtcggtgcc atcccctttg ccgtgcgcac aaataacggg tcgttaaaca
    15181 tttctctcag ccgtgccagc gcgccactga ctgctggctg ccccaagtgt aacttgctgg
    15241 ctgcacgtga aacgctcccc tctcgatgta gcaccaggag cacgattagc aggttgaggt
    15301 cgacccggtt taaatcattt tctctgatag agtccattat attaatcaat ttgaatgatg
    15361 taattcgcaa aaggatactg gtgcccgtac tgtaataaca gaacttatcg aggatagaat
    15421 gaaacttttg ctgactctgg tgacctcact ggtactctcc gtaaatgcgt tagcaacgcc
    15481 tatacctgtg ccttctcctg atacacaagc accgggctat taccgcatga tattgggcaa
    15541 actacgtatt accgctgtct ccgacggcac ggtgacaatt ccgctagata aattactcac
    15601 ccacatttct gccgataaac tgcgccagca actggcaaag gattccctga caccccatac
    15661 ggagacttca atcaatgcat ttgttattga taatggccag cagcgcgtgt tggttgatgc
    15721 cggtgcgggc gatctgtttg gcaacaatgg aggccaactg cttgaaaacc tggcagctgc
    15781 tggctatccg gctgaaacca ttgatgccgt gctgctgacg catattcatg ccgaccactc
    15841 tggtggcatc tcccgtcagg gcaaaccagc tttcccaaac gcccaggtat tcgttgagca
    15901 gaaagatgcg gatttctggc ttaatccggc gaatgcctcg aaagtagaag atagtcagaa
    15961 gcacaccttt gtcgaatcgg agcagaccat ccgcccagtg atcaacgcag gtcagctgaa
    16021 aacctttatc gcaccggcaa cggtatttgc agacattacg gccacctcaa cggccggcca
    16081 tacacctgga agcgtgatgt acacagtgga gagtgaaggc cagacgctag tgttgtgggg
    16141 cgatattatt catgccaaag cagtgcagat gccggatcct cacgtggcga tacatttcga
    16201 tgtggatcaa cagcaagcgg taaaaacccg tgagcgagta ttaaaacatg cggctcaggg
    16261 tggttactgg gtggcagctg cccatatctc attccctggc ctggggcatg ttaaaagcga
    16321 cagcaagggt tatcgttggg tcgctgccaa ctataccacg caactagccc cctgatttaa
    16381 gaggtcagtc cgcacggagc gaggatttac ggcggtattc actaaaaaga ctaattgttt
    16441 agcaggcggc cccagggttc ttcaaacggg gccacggaga gatatcactc tccctgctgc
    16501 tccagcgcat acttatacag cgcgtttttc ttcacgccgt gaatctctgc cgccagcgcc
    16561 gccgctttct tcagcggcag ttctgcctgt aacagagcca gcgtacgcag cgcatcggcg
    16621 ggtaagtctt cttcctgtgc tttatggcct tcgacaatca gcaccatttc gcctttgcgg
    16681 cggttttcat cttcctttac ccacgccagc agctcgccaa cgggcgcgcc gtgaatggtt
    16741 tcccaggttt tggttagctc acgcgccaga accacataac gggattcgcc taataccgca
    16801 acgatatctt ccaggctatc taacagacgg tgggtagatt cataaaaaat cagcgtgcgc
    16861 ggctccgctt caatggcttt tagcgcatca cggcggcctt ttgatttggc aggtaaaaag
    16921 ccttcgtaac agaaacggtc agagggtaaa cccgcggcgc ttaacgcagt gatagcagca
    16981 cacggtccgg gtagcggcac cacacgaatc cccgcttcac ggcaggtacg caccagatgg
    17041 tagccaggat cgttaattag cggcgttccg gcatcggaaa ccagcgcaat gttttgcccc
    17101 tcttgcagct tcgccagcag cgtttcggct ttttgttgtt cgttatggtc gtgcagcgca
    17161 aacaaccggg cattaatccc aaaatgttgc agcaataaac cggtgtgacg agtatcctcg
    17221 gcggcaatca gatcaacggc ctgtaatacc tctaacgcac gctgggtgat atccgccaga
    17281 ttgccgattg gcgtcggtac aatgtaaagc tggccctgag aattatccgc cgattggtgt
    17341 tgtttcattg tgtcgtccgt attgccgatt taatattgag cattgcgtaa aaaaaatatc
    17401 actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct
    17461 gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact
    17521 gcttatatgc agggcacggc gcaggctgat tctgcctttt atcttcagca gatgcagcaa
    17581 agctctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa
    17641 ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac cgcaagaact gaacgattct
    17701 cagcgtcgcg agaaaacact gctggcggta gagattaaac tggcgcagaa agattttgct
    17761 ggcgcgcaaa acttgctggc gaaaattaca cccgccgatt tagaacaaaa ccagcaagcg
    17821 cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc
    17881 gcgttaattg ctcaggaacc gctgcttggc gcgaaagaaa aacagcagaa tattgatgcc
    17941 acctggcagg cgctctcctc catgactcag gaacaggcga atacgctggt gatcaacgcc
    18001 gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgtaac
    18061 gatcccgaca tgatgaaagc cgggatcgcc gactggcaga aacgttatcc gaacaacccg
    18121 ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc agcctcgacc
    18181 aacaaaatcg ccctgctgtt gccactgaat ggccaggcag cggtatttgg tcgcactatt
    18241 cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcagc tcaggtagct
    18301 gccgcacctg ccgcagacgt agcagaacaa cctcaaccgc aaaccgtgga tggcgttgcc
    18361 agcccggcac aagcctcggt tagcgatctg accggtgaac agcctgcagc ccagccggtg
    18421 cctgtaagcg ccccggcgac aagcaccgca gcggtaagcg cacccgcaaa tccatccgca
    18481 gagctgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag
    18541 caggatggcg cgagtattgt ggtcggtccg ttgctgaaaa ataacgttga agagttgctg
    18601 aagagcaaca ctccgctgaa cgtactggca ctgaaccagc cggagaatat cgaaaatcgc
    18661 gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat
    18721 attcgtgacc agggtaaaca agcgccgctg gtgctgatcc cacgcagttc attgggcgat
    18781 cgcgtagcca atgcgtttgc gcaagagtgg cagaaactgg gcggcggcac cgttctgcaa
    18841 caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtatcgct
    18901 ttaacgggta ccccgattac tcccagagcg acaaccgact ccggcatgac gaccaacaat
    18961 ccaacgctgc aaaccacgcc aaccgatgac cagttcacca ataatggcgg tcgtgtcgat
    19021 gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat gatcgccatg
    19081 cgtaacggta gccagagcgg tgcaacgctg tacgccagct cccgcagtgc gcaagggacc
    19141 gctggcccgg atttccgact ggagatggaa ggcttgcagt acagcgaaat cccgatgctg
    19201 gcaggcggta atctaccgtt aatgcagcag gcactcagcg cggtgaataa cgattattca
    19261 ctggctcgca tgtatgcgat gggcgtcgat gcctggtcgc tggcaaatca tttctcacaa
    19321 atgcgccagg ttcagggttt tgaaatcaac ggtaataccg gaagcctgac ggctaacccg
    19381 gattgcgtga ttaacaggaa gttatcatgg ctacagtacc aacaaggtca ggtagtcccc
    19441 gccagttaac caccaaacag accggcgatg cgtgggaagt acaagcgcgt cgctggctgg
    19501 aaggcaaagg actgcgattt gtcgccgcta acgtgaacga gcgtggcggc gagatcgatc
    19561 tgataatgcg tgaaggccgg accaccgttt ttgtcgaggt gcgttaccgc cgttctgcgc
    19621 tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta ttacagactg
    19681 cccgcttgtg gctcgcgcgt cataatggga gttttgatac tgtggattgc cggttcgatg
    19741 tggtagcctt caccgggaat gaggttgagt ggattaagga tgcctttaat gaccactcat
    19801 aattaaggtt taaggattag cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt
    19861 caaactcaaa ttgcggcggc agaggcgctt ccggatgcca tctcccgtgc ggccatgacg
    19921 ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg aacttccgct
    19981 gccaatgcac agcattttgc tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc
    20041 ttacctgcca ttgcactaaa tactgataat gttgtcttaa cggcgattgc caacgatcgc
    20101 ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg
    20161 ttagccattt ccacccgtgg caacagccgc gatattgtaa aagcggtaga agctgccgtt
    20221 acgcgtgata tgaccattgt ggcattgacc ggctatgacg gcggtgaact tgcaggtttg
    20281 ttagggccac aggatgtgga gatccgcatc ccttcgcatc gcagtgctcg cattcaggaa
    20341 atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct tttccctcac
    20401 caggatgatt aaggagaata catgaaggca ttatcgccaa tcgcagtcct tatttccgcg
    20461 ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta ctgctgctgt gggtaccaaa
    20521 gccgcaactg acccacgcag tgtcggcacc caggtggacg atggtaccct ggaagtgcgc
    20581 gtgaacagcg cattgtcgaa agacgaacag attaagaaag aaacccgtat taatgtaacg
    20641 gcgtatcagg gcaaagtact gctggtaggg cagtcaccaa actctgaact ttcggctcgc
    20701 gccaaacaaa ttgcgatggg cgtagacggt gccaacgaag tgtataacga gattcgtcag
    20761 ggccagccga ttggtctggg cgaagcgtct aacgatacgt ggatcaccac caaagtgcgt
    20821 tcgcagctct taaccagcga cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac
    20881 ggtgaagtgt tcctgatggg gctggtgact gaacgtgaag cgaaagcggc ggcagatatt
    20941 gccagccggg tgagcggcgt gaagcgggta actacggcgt ttacgtttat taaatagcag
    21001 ccccttgtaa tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa cgatgcaaac
    21061 cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa tcagaataac
    21121 agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt cagccataag
    21181 gctttcgccg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac cgccgggagc
    21241 gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat cgtttgtaca
    21301 atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgctaccgc catcgccacc
    21361 acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca gacgcgagcg
    21421 gcccccaaca ccagtactgc aaggatgtaa accgggattg tactccagaa aagcgtccat
    21481 agcgccctgc cccagcggct aaaaaacccg ccctgtgctt ccggtatgtc aatttcgacc
    21541 ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc aatcagcaac
    21601 accatcacca gccccgccac cagacgaatc gccgcaaaac cccagccgag gacgaacccc
    21661 ataaacacca gcgtcgccgg gtttaacaac ggattcccca tccagaatgc cagcgcaccg
    21721 cccatcgaca cctgttggcg acgcattccc accgcgaccg gagccgcaca gcaggtacac
    21781 atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg cgattgccca
    21841 agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc gaggatcacc
    21901 cccagcaccg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc caacgccgcc
    21961 tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg ggtttcggca
    22021 gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc ggcaatgaca
    22081 acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg agatgaagac
    22141 tgaccagtca tagcattccc cagggataag ttatgataat tgagcgcgtg aatattacgc
    22201 tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga agagcttaaa
    22261 atcgtcacac cttcatgctc gggccgcatc gcctctgcca gcatgacccg cgccacgtct
    22321 ctcgcatcaa tggatttcca gttaccgggt aacaaacgga acagcggcgc aaaaagcgtt
    22381 tcgttcatcc gctgtttgct acgatcgccc aataacatcg acgggcgagc aatggtcaat
    22441 ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg gttatagaaa
    22501 aacggcgagt gggcattggc acccatagca ctgaccacca gcatatgctg cgcgcccagt
    22561 cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat aaacgcctct
    22621 ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc gatgggatcg
    22681 gtgacctgcg ccagcgcgtc agtcagttgc ggatcatggg gattaaacac cccgggcata
    22741 tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc gttaatcaac
    22801 atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac ctgactcatc
    22861 ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca ggcttaatag
    22921 accatgaggt aattatcccc gattgtgaga aattcgcctc atccaatgca acaacgcgga
    22981 ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgaaga ttcagaattt
    23041 acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat tgaaaaacaa
    23101 gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga taaatccatc
    23161 gatgaagtga cgccagcgga gtttgatgcc ctgttgctac cgggcggtca ttcaccggat
    23221 tatctgcgtg gcgacaaccg ttttgtcacc tttacccgtg attttgtgaa tagcggcaaa
    23281 cctgttttcg ccatctgtca cggtccgcag ctgctgatca gcgccgatgt gattcggggg
    23341 cgcaaactga ccgcagttaa accgatcatt attgatgtta aaaatgcggg cgcggaattt
    23401 tacgatcagg aagtcgtggt tgataaagat cagctggtta ccagccggac gccggacgat
    23461 ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagccgc gtagccaaat
    23521 cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga gccggatttc
    23581 ccacaccggg gccgacagca ttctggcaac cggaaagcga cgattgtacg ccttgcgcgc
    23641 gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca cgccgcgaat
    23701 taacgctacc gttttcggct gaccgtttac cgttccggca accgcagcct gcggcccact
    23761 catctgcgca tgacgcgttt tttcttccgt caaaatgtag aaggcgactt tctgcgcatc
    23821 aaacagataa aaagcattag cgcaccagag ttccccttcc tgctgcacac accaggtgac
    23881 aacatgttgt tttgccaacc aacggctgat ggtgatgagt gtttccattg ctgttctctt
    23941 ttatactgtg ggcctgacct taacatgcga cgcagacgat gacaccctgg tttctttact
    24001 tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc gaacgccgct
    24061 atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga gaactgacgc
    24121 tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt acgggcggaa tatcgcgtta
    24181 aacaactgac caaacggcaa aaagagcgtc tggtagcgga aggcgcaggg tttgcagagc
    24241 tgttaagcag tctgcaaacc ccggagatta aaagcgattg aaatgctcgt gatactcaac
    24301 cagaccagtt acgccattca acgcgtcatc cgctaaacga tgtacctgga aggcgctttc
    24361 agtgcctggc cagcggcaac gcagatcgtg atgtgccgcc agttcaaagc caaaacggct
    24421 gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat taagcgaatc
    24481 gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt catcaaccgc
    24541 caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg gactaaatgc
    24601 cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg tcagaaagcc
    24661 atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg agcgacgcag
    24721 cagggcatca atacccggcg catcaatggg aatttctact cgaattagca tggttcacct
    24781 accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa atccgccagt
    24841 tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc gtccatcagg
    24901 tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg ctggaagaag
    24961 agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc gtcggcacta
    25021 aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac caccgaggta
    25081 aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc aagaaactcc
    25141 agctcgccat catccagcgc ctgacggaat tgccagctta agacctgctc aagaacctgg
    25201 cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg ccccaaatgc
    25261 acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa caatcatcct
    25321 attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat atgaaattgc
    25381 tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc aaggttaact
    25441 aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg aaaaattatg
    25501 gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga aaacggcgca
    25561 gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc cggccttaac
    25621 tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg ccgcaaactt
    25681 cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca gcgcgccgtg
    25741 gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc catgctggag
    25801 tacgccgccg agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc ttcggcgacc
    25861 aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgcccgcgt ggtgctgccg
    25921 cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt cccgctggaa
    25981 gtgtttgctt tcggcagcct gtgcattatg tcggaagggc gttgctatct gtcgtcgtat
    26041 ctgacgggtg agtcgcccaa caccgtgggc gcgtgttctc cggcccgttt cgtgcgctgg
    26101 cagcaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga ccgttatcag
    26161 gacggcgaaa acgcaggtta tccgacgctg tgtaaagggc gttatctggt ggacggcgag
    26221 cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct gccggagtta
    26281 atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc ggcgtatgtc
    26341 agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga tccgcaaaac
    26401 ttcgtaccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg cacgcaaacc
    26461 actcttggcg catatcaccg taaatggcag tgagaaaagc aatgaaatat tccttagggc
    26521 cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag gccgccacca
    26581 gcagcgccga cgtgatttat cttggcgaag cggtatgcag caagcgccgg gcaaccaaag
    26641 ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggtaagcag atagtgctct
    26701 ccacgctggc gctggtgcag gcatcatctg aactgggcga actgaaacgc tatgttgaga
    26761 acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc gctgaacgca
    26821 aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgaca ctgaaaatat
    26881 tgctcaaaca gggcatgatg cgctggtgta tgccagtgga gctttcccgc gactggctgg
    26941 tgaatctgct taatcagtgc gatgagctgg gcattcgtaa ccagtttgaa gtggaagtcc
    27001 tgagctacgg tcatctgccg ctggcctact ccgcccgctg ctttaccgcg cgttcggaag
    27061 accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg cgcaacgtgc
    27121 tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg agcggctacg
    27181 tttacaacct cggtaacgag ctggcatcca tgcagggcct ggttgatgtg gtccgcctgt
    27241 caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat gaaaatggcg
    27301 ctgcaccact gccgttgacg gcgaacagcg actgtaacgg ctactggcgg cggctggcag
    27361 gactggagct gcaagcctaa gtaaatagct cactttgtta acaactttaa ctactcttta
    27421 atgcagtatt aaagattaat cggtaacaaa gtgagctgtt atgacggata aaaccattgc
    27481 gttttcgcta ctcgatctgg cccccattcc cgaaggttct tcagcgcgag aagcattctc
    27541 ccactctctc gatctcgccc gtctggctga aaagcgcggc tatcatcgct actggctggc
    27601 agaacaccac aatatgactg gcattgccag tgctgccacg tcggtattga ttggctatct
    27661 ggcggcgaat accaccacgc tgcatctggg gtctggcggc gtgatgttgc ctaaccactc
    27721 accgttggtc attgcagaac agttcggcac gctcaataca ctctatccgg ggcgaatcga
    27781 tttggggctg ggtcgtgctc cgggtagtga ccaacggaca atgatggcgc tacgtcgtca
    27841 tatgagcggc gatattgata atttcccccg cgatgtcgcg gagctggtgg actggtttga
    27901 cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc tacggcgagc aaatccccgt
    27961 gtggttgtta ggctccagcc tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt
    28021 tgcgtttgcc tcacacttcg cgccggatat gttgttccag gcgctgcatc tttatcgcag
    28081 caacttcaaa ccgtcggcac gactggaaaa accatacgcg atggtgtgca tcaatattat
    28141 cgctgccgtc agcaaccgcg acgctgaatt tctgtttacc tcaatgcagc aagcctttgt
    28201 gaagctgcgc cgtggcgaaa ccgggcaact gccgccgccg attcaaaata tggatcagtt
    28261 ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg agtatgtcgc tggtgggcga
    28321 taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg ccgatgagat
    28381 tatggttaac gggcagattt tcgaccacca ggcgcgcctg cattcgtttg agctggcgat
    28441 ggatgttaag gaagagttgt tgggatagtg tgtcttaacg cgggaagcct tatccgagct
    28501 ggcaacgctg tcctacgtag gcctgataag cgaagcgcat caggcattgt gtaggcagca
    28561 gaaatatcgg ataaggcccg ctgattactg atacaccggc agcaaattaa agctcgataa
    28621 gatatggacc agtgcgttgc caacgccaaa taccagaatc agcgcaatca tcggcttgcc
    28681 accccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ccagcagtgc
    28741 cggaacaatt gccgcccaga tggtagccgc taaaccagca taaccaatgg cgtacaggaa
    28801 tccgttcggg aacaacagcc cccccacaac tggcggggca aaggtcagca atgccgtttt
    28861 caagcggccc acagccgagt cgtcgaaacc aaacagatct gccagatagt caaacaaacc
    28921 cagcgttacg ccgaggaacg aactcgctac cgcaaagttt gagaacacga ccagcagcag
    28981 atccagacta cggctgttca gtacgccgct taacgcctgt accagcacat caatattacc
    29041 gcccttctct gcaataccga taaactccgg acgcgggatg ttacccatcg tcgccagcaa
    29101 ccagatggta tacagcgcca gcgccatcag cgtaccgtac accagacatt tcacgatggt
    29161 tttcggatct ttgccgtaat acttcatcag gcttggcacg ttaccgtgat aaccaaacga
    29221 tgccagacag aacggcaggg tcatcaacag atacggtgca taagacgcat tgctttcggc
    29281 gacgttgaac aatgtcgcag gctgcacatg ccccagcagg ctaccaaagg tgaggaagaa
    29341 ggtaatgact ttcgccccca gcacaatcgc cgtcatgcgg ctcacggctt tagtgctcaa
    29401 ccacaccaca aacgccacca gcagtgcaaa accaaaacct gccgcccgtg ccgggacgtt
    29461 tagcgacatc tctgcgaagg tatgatgcag aatcgaacca ctggcagaaa tataggcgta
    29521 ggtcaggata tagagcacaa aggcaatgga aatgccgttg accacgttcc agcctttgcc
    29581 cagcaaatct ttggtgatgg tgtcaaagct cgaaccgatt cggtaattca ggttagcttc
    29641 cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg ccgccattga
    29701 ccagaagaac cacgccccgg acatgaccac tggcagagaa aacatccctg cgccaataat
    29761 ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttag
    29821 tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgtgttgta
    29881 ctgtaccagt acacgagtac aaaagacaga aaaaaagccc cgatggttaa aatcggggct
    29941 gtatatatta ttttacagac tgtgttcgct gttcagcgat gattacgcat cgccaccgaa
    30001 acgacgacga ccggtagagt catcacgacg cggtgcgcgg ccttcacgac gttcgccgct
    30061 aaaacgacga ccatcaccac gaccaccttc acggcgttca ccgctgaagt tacgaccgcc
    30121 ctctcgacgc tcgccactaa aaccacgacc accgccacga cgctcaccgc cagtatgcgg
    30181 ctgtgcatcg cccagtaact gcatattcat cggcttgttc agaatgcgag tacgcgtaaa
    30241 gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt gagaagcaaa
    30301 cagcttgatg ttaccgatgt aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac
    30361 gatatgacga acttcaacac catcatcgcg gcccacttca atgcggtaca gctgcatgtc
    30421 accaacatca cgacgttcac gacgcggacg atcttcacgg tcgccacgcg ggccacggtc
    30481 gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg
    30541 cgcatcaggc ggaacaatca gagtacgttc accctgtgcc attttcagca gtgccgcagc
    30601 cagagtttcg agatccagct cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc
    30661 gcggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc
    30721 cagacggcgt ttgcccagca gttctgcgtt cggcagttct acttccggaa tagtcagctt
    30781 catagtacgt tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga acaacagcgc
    30841 gcggccagca cgacccgcac gaccggtacg accgatacgg tgaacgtaag actcagaatc
    30901 catcgggata tcgtagttaa ccaccaggct gatacgctca acgtccaggc cacgggctgc
    30961 aacgtcggtc gcaatcagga tgtccagacg accatctttc aggcgttcca gtgtctgctc
    31021 acgcagcgcc tggttcatgt caccgttcag cgcggcgctg ttgtagccgt tacgctcaag
    31081 agcttcagcc acttccagag tcgcattttt ggtacgaacg aagataatcg ccgcatcaaa
    31141 atcttccgct tccaggaaac gtaccagtgc ttcgttttta cgcatacccc agacagtcca
    31201 gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatgcgca cttcctgcgg
    31261 ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag
    31321 agcggtctga tgaccttccg ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc
    31381 catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag agaggtccag
    31441 agtgccacgt ttcaggtggt ccagcagacg gcccggagta ccgacaacga tctgcggccc
    31501 ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacaggg caaccacatt
    31561 tacgccgcgc atgtgtttag agaaatccgt cattgcttca gcaacctgta ccgccagttc
    31621 gcgggtcggt gccagcacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcag
    31681 cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc
    31741 gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc
    31801 cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc
    31861 gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat
    31921 catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta
    31981 acgaacaaca acgccctcac ccgttaaggt gatggcaatc aaaaaagatt acgggctgat
    32041 gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc
    32101 gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc
    32161 agtttgaaca gtgccgtggc gctgtccaaa tcccccagac ttaggtagta cttacctaaa
    32221 tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg
    32281 agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac aatgttccat
    32341 ccccactgtt ccttatccga tttttcgaag tgctgtttca acacttcttt agcctgcttc
    32401 tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg
    32461 tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat
    32521 gcgatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca
    32581 aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg
    32641 aatacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat
    32701 gccctcatac cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg
    32761 tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc
    32821 tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg
    32881 catcctgcaa gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc
    32941 cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg
    33001 cccggttaaa agccccccgc cgcagcggag ggcaaatggc aaccttactc gccctgttca
    33061 gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc
    33121 agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc
    33181 tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat gtgtaccaga
    33241 ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta
    33301 ccattgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagcg
    33361 tgtttcgctt tctcgccgtc ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg
    33421 atggtggtgc cagtttcttc ggtcagagca cggattacag aaccgccttt accgataaca
    33481 tctttgatct tgtccgggtt gatcttgatg gtatggatac gcggtgcgaa ctcagagata
    33541 tcgccacgcg gcgcgttgat cgcctgttcc attactccca ggatatgcag acgcgcacct
    33601 ttagcctggt tcagcgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc
    33661 atctgcaatg cagagatacc gtcgcgggaa cccgcaactt tgaagtccat atcgcccagg
    33721 tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga
    33781 cccattgcga tacccgcaac ggcagctttg attggcacac ctgcgtccat cagcgccaga
    33841 gacgcgccgc acacggaagc catagaagaa gaaccgttgg attcggtgat ttcagacact
    33901 acacgtacgg tgtacgggaa tttgtccata tccggcatga ctgccagcac gccgcgcttc
    33961 gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct
    34021 acggagtacg gagggaagtt gtagtggaac aggaaggtat cggtacgttc gcccatcagt
    34081 tcatcaagaa cctgcgcgtc acgagcagta cccagcgttg cggtaaccag cgcctgcgtt
    34141 tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc
    34201 agaccacgga tcatatcttt ttcacgacca tcgatacgcg gttcgcctgc cagtacgcgg
    34261 ctacgaacaa cgtttttctc gatcgcgtgc agaatttcac ccagttcgtt ttcgtccagg
    34321 gtttcgtctt cagcaagcag cgttgcgatg gtttcagatt tgatgacatc aacctgcgca
    34381 taacgctctt gtttgtcggt gatgcggtaa gcatcgctca gacgagcttc agccagtgca
    34441 gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgcggttta
    34501 ccggcttctt ttaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg
    34561 aacactactg cgcccagcat ctggtcttcg ctcaacagtt cagcttccga ttcaaccatc
    34621 agcacggcag cttcagtacc ggcaaccacc aggtccagct tgctctcttt cagctcgtcc
    34681 tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgggcagc accaatcgga
    34741 ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata
    34801 tccgggttaa cttgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg
    34861 aagccttccg ggaacagcgg gcgaatcggg cgatcaatca gacgcgcgat cagggtttcg
    34921 ccttcgcttg gacgaccttc acgacggaag aagctacccg ggatacgacc agcagcgtag
    34981 gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggttt ggcttttttc
    35041 tggccaacaa cggtaacaaa taccgcggtg tcatccatgc taaccataac agcggcagta
    35101 gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta
    35161 cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagca ccttacggca
    35221 ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct
    35281 ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag
    35341 caacaataca ttagtttcca gtgaattgct gccgtaagct tgaaaaaagg ggccactcag
    35401 gccccctttt ctgaaactcg caagaattag cgacgcagac ccaggcgctc gatgagctgg
    35461 gtgtaacgtg ctacgtcttt acgtttcagg tagtcgagca gtttacgacg ctgagaaacc
    35521 atgcgcagca gaccacgacg gctgtggtga tcttttttgt gctctgcaaa gtggccctgc
    35581 aggtggttga tctgtgcagt cagcagtgct acctgaactt cggtagaacc ggtgtcgttt
    35641 gcgtcacgac caaactcaga aacgatttta gctgttgctt cagtacttag agacatttta
    35701 aaactccaaa gtatatagaa tgaaaggacg ccgatctcta attcagcgat ccgagtgtac
    35761 gttacgcaaa gtgttaaaca atttacgcga cgttaagcgg cagtattcta ctcgtagcga
    35821 cctgttatcg caagacggtt aacattacgc cgggtattca accaccaggc gacgaggcgc
    35881 aacgcggcct tcatcgtcaa tttcgcccat accgataaat ttgccgttct caccttccgt
    35941 gacgcgaacc agtccttcca gcggcgcacc agatgtacga accgggttac catttttgaa
    36001 gtaaacagaa gacgttaacg gaagattcac caccggatag tccgaagctg gactgtccat
    36061 tggcatcagt aatggatcaa gtaactccgc agccggaata tcctgctgtt cagcttgctc
    36121 aacaagttca cgcaggtgct ccagggtcac catccgttca atcggatatt tacttaccgc
    36181 cagacggcgc aggtaaataa catgcgcgcc acagccgagt ttctcaccca ggtcgtcaat
    36241 gatagtgcgg atataagtgc cttttgagca gtgaatttcc agctccagct cattgccttc
    36301 atggcgaata aacagcaatt cataaacggt aatcggacgc gcttcacgcg gaacttcaat
    36361 gccctgacgc gcatattcgt acagtttttt gccctggtat ttcagtgctg aatacatcga
    36421 tgggatctgt tcgatatcgc cacggaaagt atccagtgcc gccgccagtt gttctgcact
    36481 aaaggttacc ggacgctctt caacgatttg tccgtcggcg tcagaagtat cggtacgctg
    36541 tccaagacgc gcaatgaccc gatagcgttt gtcggagtcc agcagatact gggaaaactt
    36601 cgtcgcttcc ccgaggcaaa tcggcaacat gccggtcgcc agcgggtcca gcgcaccggt
    36661 atgcccggca cggttggcgt tataaatccg tttcactttt tgcagcgcat cgttgctgga
    36721 cataccctga ggtttatcca gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg
    36781 acgactcatt agtcctcctt gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg
    36841 accacgctgg tcaccaggtt tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag
    36901 aaggtcagtt ccggcacgat acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa
    36961 ccagaagctt cttgcaacgc tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg
    37021 aggaacgtca catatacttt ggcatacgcc aggtcgcgag acatttcgac accggaaacg
    37081 gtggtcatca tgcccaggcg aggatcttta atttcacgct gcaggatgag agcgatctct
    37141 ttttgcattt cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat aataaattct
    37201 cctgacaaaa aaggggctgt tagccccttt ttaaaattaa tttcaggtgg aagggctgtt
    37261 cacgttggcc tgataagacg cgccagcgtc acatcaggca atccatgccg gatgcggcgt
    37321 aaacgcctta tcccgcatgg aaccctaaaa accttaagcg atggtacgtt ggatctcgat
    37381 gatttcgaat acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat
    37441 accacattcc ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc
    37501 cagctcgcct tcatagataa ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt
    37561 aaccacacct tcggtaacca tacagcctgc gatggcacca aatttcggcg atttgaacac
    37621 gtcacgaact tccgccagac cgataatctg ctgtttcagt tccggagaca gcataccgct
    37681 catcgccgct ttcacttcgt cgatcaggtt atagatgacg gagtagtaac gcagatccag
    37741 gctttccgct tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaactag
    37801 gatggcgttg gacgccgcag ccagggtggc gtcggtttcg gtgataccac ctacgccaga
    37861 accgatgatc ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatcgc
    37921 ttccacagaa ccctgtacgt ctgccttcag gacgatattc acttcgtgaa cttcgccttc
    37981 ggtcatgttg gcgaacatgt tctcgagttt agatttctgc tgacgcgcca gtttaacttc
    38041 gcggaattta ccctgacgat agagtgcaac ttcacgcgct ttcttctcgt cacgtacaac
    38101 ggtaacttca tcacccgcag ccggaacacc ggacaagccg aggatttcca ccggaatgga
    38161 cggacccgct tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta
    38221 ttcgaagcca cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt
    38281 agcaaccgga ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatacc
    38341 tttacgtacc gctttcagct ccaaaacttc cgcctgcagc aggatagcgt ccagcagttc
    38401 atcgataccg gtacccgctt tcgcagatac gtgtacgaac tggctttcac cgccccactc
    38461 ttccggcagg atgccgtact gggagagttc gttcttaacg cgatccggat cggcttctgg
    38521 tttatcgatc ttgttcactg caaccaccac cggtacctgc gccgctttcg cgtgctggat
    38581 tgcttcgatg gtctgcggca ttacgccgtc gtcggcagca acaaccagga ctacgatgtc
    38641 cgttgcctgc gcaccacgtg cacgcattga agtaaacgcg gcgtgccccg gggtgtccag
    38701 gaaggtgatc atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc
    38761 gcccgcttcg ccagaggcca ctttcgttga acgaatgtag tccagcagag aggttttacc
    38821 gtggtcaacg tgacccatga tggtcacaac cggagcgcgc ggttcagccg cagcccccgt
    38881 gtcacggtcg ctcattaccg cctcttccag ctcgttttca cgacgcagga taactttgtg
    38941 gcccatctct tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc
    39001 cattgcgccc agtttcatca tcgctttgat gacctgagag cctttaaccg ccatcttgtt
    39061 cgccagttcg ccaacggtga tagtttcgcc gatcacaacg tcacggttaa cggcctgagc
    39121 aggcttctgg aagccttgct gcagcgaaga acctttacgt tttccgccct taccgccacg
    39181 tactgctgcg cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tgcctttctt
    39241 cggacgcgct gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct
    39301 ttcgtcttct gcctggcgag catgttgaga agtagtgacg tgataatcgc tggaatcctc
    39361 agtcggttcc gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc
    39421 aacgcgacgt gcttcttctt cgagtttacg acgcgcttct tcttcggctt tacgcttgag
    39481 ctctgcagct tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc
    39541 ttgttgattg ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc
    39601 acgcttagct tgttctgcgg cctcacgttc agctttttgt tgcgcctcgc gtttagccga
    39661 ttcttctgcc tcacgacggg cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc
    39721 aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat
    39781 ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc gtgttttacg
    39841 ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg
    39901 tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat cagcaaattg
    39961 ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc
    40021 tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc agcaaatatt
    40081 acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagccag
    40141 atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg cggccagttt
    40201 gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg gtttgttatc
    40261 accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag cacgctcgcg
    40321 cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt tcatcggcac
    40381 ataggccagt tcttccagcg tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc
    40441 gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt
    40501 agcctgcagg tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagct gcgaagccag
    40561 acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccggctt caacggcgat
    40621 atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg gtgccatagc
    40681 gttaatcacg aactgcgccg ggttatcatc ccacaggacg atatcgatac gctcgccgcc
    40741 cagttcagta gaaaccgcct gaacacgtgc gccacgcata cctacgcaag cacctaccgg
    40801 atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc
    40861 cgctgcttta atttcaatca cttcttcgcc gatttctggc acttcaatac ggaacagttc
    40921 gatcagcatt tccggcttgg aacgggtgac gaacagctgc gcaccacgcg cttccgggcg
    40981 aacggaataa agcacgccac gaacacggtc gccagggcgg aagttttcac gcggcagcat
    41041 atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg
    41101 gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga actgatcaac
    41161 caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg ccgtctgggt
    41221 agtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc ccaggttcag
    41281 gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct gggtgacttc
    41341 atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac
    41401 acggacgtcg atctcttgtt catatttttt ctttgttgct gtcgccagcg cgctttccaa
    41461 tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt caactacagc
    41521 caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa agtggggaac
    41581 caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt cgacggtaac
    41641 tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac ggttttgtac
    41701 cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt
    41761 gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa cggtgatggg
    41821 atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat catcaacatt
    41881 gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc gaataaattc
    41941 gatgccaacc agttcaaaac ccagggcctc aactggcgca gtaatcatct ctgttaattt
    42001 ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa agcccagtta
    42061 ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac tgaaccctat
    42121 aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc actacgaagg
    42181 ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg cgggggccgg
    42241 atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc
    42301 gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact gctgggattt
    42361 ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccatgc cacattttgt
    42421 caacgtttat tgctaatcac gtgaatgaat atccagttca ctttcatttg ttgaatactt
    42481 ttgccttctc ctgctctccc ttaagcgcat tattttacaa aaaacacact aaactcttcc
    42541 tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa attcagtgag
    42601 agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat gacgacgatt
    42661 ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg tctggacacc
    42721 agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata tactgcaaac
    42781 ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat ggaatacggc
    42841 gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg tattgccgct
    42901 attcagtgtg gcgcatttca taacaccacc ggtggcctga cctatttcaa cacgacgccg
    42961 ctgggccgcg ccgtgactgg caccatgctg gttgctgcta tgaaagaaga tggcgtgaat
    43021 atctggggtg acggcagcac ctataaagga aacgatatcg aacgtttcta tcgttacggt
    43081 ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga ctttattgat
    43141 gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt cgactacaaa
    43201 atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac gcatgaagcg
    43261 aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa
    43321 ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt tgagcaaggt
    43381 catccggtgg cgctgaacgg taaaaccttt agcgacgacg tagaaatgat gctggaagct
    43441 aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgaaaa ccgtatcatc
    43501 gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg cactgctgca cattgcgtat
    43561 gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca cgcgcatggt
    43621 cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc gctgatgctg
    43681 cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac cctggagctg
    43741 cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac ctacaagcca
    43801 gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg tattggtcaa
    43861 ttgaccatgc gtaacctgga tatcaccgat acccgcgaga aacttttcgg ttatgccaaa
    43921 accggcctgc tttcctcctc tgccgcttca ggcgtgccgc aggtggataa tctggaaaac
    43981 aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct tgcttatcct
    44041 ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca tccacaaccc
    44101 gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt tccggctttg
    44161 ttactcccat ccacgcatta atcaggtaat tattgtaagc tgtcgaaaag atgttgtttt
    44221 ccgttgtgcg atccacacca tccccaagaa cggggctata ccagataaac atcggaacat
    44281 gatatgcctg ctggctggcc tccctgcctc catgaaaata gacgttcttc ttcgtagggt
    44341 cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct tttaataatt
    44401 caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag caggcatcct
    44461 gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa gccggttcat
    44521 ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt
    44581 gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcac
    44641 gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc
    44701 agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa
    44761 tgtcatggct caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag
    44821 gtgcaccact tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcgacttgcg
    44881 gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca
    44941 caatcaacac gtaggtatca atacctgtat ccctgactga taattgaaaa tacggcactg
    45001 tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa taattgagat
    45061 tgaaaaacgg cgtataggta gcaaatcgcg acgctagtat atatggactg aacgcatttt
    45121 tatttttggc atctttataa gcaaattgac aagcggaaaa taaacttccc gaaatgacaa
    45181 tcagcaataa tattcctgtc acttttttgt cggcaaggaa acatcatatt ttattattac
    45241 agccaaaaaa agaagggata aaaaggcaaa agcacataga taagggctat acattcccag
    45301 cattttgaca acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa
    45361 agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa
    45421 cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag
    45481 gatggtcaga acgaagaaca gcaatcgctg aaccatatgc gctccggaag aggcgactaa
    45541 gttcgtaata ccaaaaacaa taacactaag atacaacaac caatgagatt taaaacttct
    45601 agcaaatttg ttgaatactg tcatccagaa atcc
//
LOCUS       DAZUGK010000014        44916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZUGK010000014 DAZUGK010000000
VERSION     DAZUGK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.014"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..539
                     /gene="lpfA1"
                     /locus_tag="ACHY5W_000683"
     CDS             15..539
                     /gene="lpfA1"
                     /locus_tag="ACHY5W_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFS4445638.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFTGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLKTGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            604..1290
                     /gene="lpfB"
                     /locus_tag="ACHY5W_000684"
     CDS             604..1290
                     /gene="lpfB"
                     /locus_tag="ACHY5W_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFS4445639.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQIFANDKEIEKAGMVPAKGSLNITLPAGVGSVSKIKYNAINDFGS
                     SSEMMTKNVD"
     gene            1319..3886
                     /gene="lpfC"
                     /locus_tag="ACHY5W_000685"
     CDS             1319..3886
                     /gene="lpfC"
                     /locus_tag="ACHY5W_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFS4445640.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASAFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYDDDNGAIHHDDGDNTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGALISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWSKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAVVPYATPYRENEISLRSDSL
                     GDDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQNE
                     VSSIVGEEGQLYISGMPEEGRILIKWGNDASQQCVAPYKLSLELKQGGIIPVSANCQ"
     gene            3901..4956
                     /locus_tag="ACHY5W_000686"
     CDS             3901..4956
                     /locus_tag="ACHY5W_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445641.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPVDGTTYHYNVDVD
                     VGVPDASKNVAGTVLTDVMNWSNGQNVSIICECPDSYKNEKDTLIQGVSAIANMTKDI
                     GGFKYYYLTDELSVSTKIQVSSGQYGFVPFKGQQALQVTGCNEVITTPYMGGGGLLSF
                     LITKPFIGDSVIPPTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAVIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            4961..5491
                     /gene="lpfE"
                     /locus_tag="ACHY5W_000687"
     CDS             4961..5491
                     /gene="lpfE"
                     /locus_tag="ACHY5W_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFS4445642.1"
                     /translation="MKFKLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5746..7437
                     /gene="eptB"
                     /locus_tag="ACHY5W_000688"
     CDS             5746..7437
                     /gene="eptB"
                     /locus_tag="ACHY5W_000688"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFS4445643.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYISPDGGINENNNWCHIPQTKEAAAN"
     gene            7529..7605
                     /locus_tag="ACHY5W_000689"
     tRNA            7529..7605
                     /locus_tag="ACHY5W_000689"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Pro,seq:cgg)
     gene            8348..9955
                     /gene="dppA"
                     /locus_tag="ACHY5W_000690"
     CDS             8348..9955
                     /gene="dppA"
                     /locus_tag="ACHY5W_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFS4445644.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10106..11125
                     /gene="dppB"
                     /locus_tag="ACHY5W_000691"
     CDS             10106..11125
                     /gene="dppB"
                     /locus_tag="ACHY5W_000691"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFS4445645.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11135..12037
                     /gene="dppC"
                     /locus_tag="ACHY5W_000692"
     CDS             11135..12037
                     /gene="dppC"
                     /locus_tag="ACHY5W_000692"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFS4445646.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12048..13031
                     /gene="dppD"
                     /locus_tag="ACHY5W_000693"
     CDS             12048..13031
                     /gene="dppD"
                     /locus_tag="ACHY5W_000693"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFS4445647.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13028..14032
                     /gene="dppF"
                     /locus_tag="ACHY5W_000694"
     CDS             13028..14032
                     /gene="dppF"
                     /locus_tag="ACHY5W_000694"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFS4445648.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14062..15333)
                     /gene="yhjV"
                     /locus_tag="ACHY5W_000695"
     CDS             complement(14062..15333)
                     /gene="yhjV"
                     /locus_tag="ACHY5W_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFS4445649.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGVLLCLSPLLKLIE"
     gene            15809..15916
                     /gene="ldrD"
                     /locus_tag="ACHY5W_000696"
     CDS             15809..15916
                     /gene="ldrD"
                     /locus_tag="ACHY5W_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4445650.1"
                     /translation="MTLAELDMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16003..17682)
                     /gene="bcsG"
                     /locus_tag="ACHY5W_000697"
     CDS             complement(16003..17682)
                     /gene="bcsG"
                     /locus_tag="ACHY5W_000697"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFS4445651.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17679..17870)
                     /gene="bcsF"
                     /locus_tag="ACHY5W_000698"
     CDS             complement(17679..17870)
                     /gene="bcsF"
                     /locus_tag="ACHY5W_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFS4445652.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(17867..18658)
                     /gene="bcsE"
                     /locus_tag="ACHY5W_000699"
                     /pseudo
     CDS             complement(17867..18658)
                     /gene="bcsE"
                     /locus_tag="ACHY5W_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312443.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
     gene            18650..19102
                     /locus_tag="ACHY5W_000700"
                     /pseudo
     CDS             18650..19102
                     /locus_tag="ACHY5W_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
     gene            19099..21717
                     /gene="bcsA"
                     /locus_tag="ACHY5W_000701"
     CDS             19099..21717
                     /gene="bcsA"
                     /locus_tag="ACHY5W_000701"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFS4445653.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            21728..24067
                     /gene="bcsB"
                     /locus_tag="ACHY5W_000702"
     CDS             21728..24067
                     /gene="bcsB"
                     /locus_tag="ACHY5W_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFS4445654.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNLMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            24074..25180
                     /gene="bcsZ"
                     /locus_tag="ACHY5W_000703"
     CDS             24074..25180
                     /gene="bcsZ"
                     /locus_tag="ACHY5W_000703"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFS4445655.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKGQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            25162..28635
                     /gene="bcsC"
                     /locus_tag="ACHY5W_000704"
     CDS             25162..28635
                     /gene="bcsC"
                     /locus_tag="ACHY5W_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFS4445656.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQKKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSIWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLHQQPPSSRIDLSLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAVAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            28717..30705
                     /gene="hmsP"
                     /locus_tag="ACHY5W_000705"
     CDS             28717..30705
                     /gene="hmsP"
                     /locus_tag="ACHY5W_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFS4445657.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLQEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            30888..32174
                     /gene="dctA"
                     /locus_tag="ACHY5W_000706"
     CDS             30888..32174
                     /gene="dctA"
                     /locus_tag="ACHY5W_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFS4445658.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            32394..33890
                     /gene="yhjJ"
                     /locus_tag="ACHY5W_000707"
     CDS             32394..33890
                     /gene="yhjJ"
                     /locus_tag="ACHY5W_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HFS4445659.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEHYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(33986..34915)
                     /gene="kdgK"
                     /locus_tag="ACHY5W_000708"
     CDS             complement(33986..34915)
                     /gene="kdgK"
                     /locus_tag="ACHY5W_000708"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFS4445660.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRCFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            35147..35914
                     /gene="pdeH"
                     /locus_tag="ACHY5W_000709"
     CDS             35147..35914
                     /gene="pdeH"
                     /locus_tag="ACHY5W_000709"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFS4445661.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            35984..38044
                     /gene="yhjG"
                     /locus_tag="ACHY5W_000710"
     CDS             35984..38044
                     /gene="yhjG"
                     /locus_tag="ACHY5W_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS4445662.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKNANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEFTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGKGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(38226..39548)
                     /gene="yhjE"
                     /locus_tag="ACHY5W_000711"
     CDS             complement(38226..39548)
                     /gene="yhjE"
                     /locus_tag="ACHY5W_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4445663.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(39941..40954)
                     /gene="yhjD"
                     /locus_tag="ACHY5W_000712"
     CDS             complement(39941..40954)
                     /gene="yhjD"
                     /locus_tag="ACHY5W_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFS4445664.1"
                     /translation="MTQENEIKRPTQDLEHKPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(41003..41902)
                     /gene="rcdB"
                     /locus_tag="ACHY5W_000713"
                     /pseudo
     CDS             complement(41003..41902)
                     /gene="rcdB"
                     /locus_tag="ACHY5W_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            42422..43024
                     /gene="yhjB"
                     /locus_tag="ACHY5W_000714"
     CDS             42422..43024
                     /gene="yhjB"
                     /locus_tag="ACHY5W_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4445665.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(43075..44724)
                     /gene="treF"
                     /locus_tag="ACHY5W_000715"
     CDS             complement(43075..44724)
                     /gene="treF"
                     /locus_tag="ACHY5W_000715"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS4445666.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT        10790 a        11261 c        12270 g        10595 t
ORIGIN      
        1 aaatggagtt tttcatgaaa aaggtcgttt ttgccttaac cgcactggcg ttaacttctg
       61 gcactgtatt cgcagcagag tctggtgacg gcacggttaa atttacaggc gagatcgtgg
      121 attcaccttg cgtactgtcc gttgactctc agaaccagga agttgtgttg ggtcaggtac
      181 aaaaatctgt atttgctgcg gttggtgaca aatccccggc gaaacctttc gaaatcaaac
      241 tggaagattg cgataccaca acgatgaaaa aagccaatgt gagctttact ggtgttggtg
      301 atgcagataa atctgatctg atttctgtta gcactgaagc aggtgcggcc aaaggtgttg
      361 gtatcggtat ttatgataac agcaacaccc tggtcgctct taacggtggt aaagcaagtg
      421 ttgacctgaa aacaggccag acggttctct actttaccgc caactatgtt tcaactctgg
      481 caacggtaac gaccggttac ggcaatgcac aggtcgattt caacctgtct tacgagtaat
      541 cgtccgtttt acaaatggcg gcgtaattgc cgccattagt cgtcaaagtt catggatcga
      601 atgatgaaaa gtaaatttgt tgcgttggct ctctctctgt tcctttcaca gtcagttctg
      661 gcgggtggcg tcgggcttag ctctactcgc gtaatttatg atggcagtaa gaaagaagcc
      721 tcgctgaccg tacagaataa aagtaagacg gaagagtttc ttatccagtc ctgggtggat
      781 gatgccgcgg gcagcaaaaa aacaccgttt attattacac ccccactatt caagcttgat
      841 ccagaaaaga acaatatttt gcgtattgtg aatattacgc ccggcctgcc gcaggatcgt
      901 gaatcggtct actgggttaa tgtgaaagcc attccatcta aaagtgacga cagcgaaaac
      961 aaaaatgttt tgcagattgc cgttcgcacc cgaatcaaac ttttttatcg cccggcaggt
     1021 ttaaagggtg atgtaaaaac cgcgccaaat gaactgcgat ttacgcgtaa cggtaatcag
     1081 ctccgcgttg ataatccgac agtttttaat attaccttta atcaaatttt tgcgaatgac
     1141 aaagaaattg aaaaagcagg catggtaccc gctaaagggt cattaaatat cacattaccc
     1201 gccggggtag gtagcgtttc taaaattaaa tataacgcca tcaatgactt tggatcatct
     1261 tcggaaatga tgactaaaaa tgttgattga ttaatatctc accatgagaa atagctcaat
     1321 gtcacggaag accgtttcac gtactttttc ttccttttct atctctgtgg tggcggtcgc
     1381 ggttgccagc gcgttttctg cacatgccgg aaagtttaac cccaaattcc tggaggacgt
     1441 tcagggtgtg ggccagcatg tcgatttaac gatgttcgaa aaaggccagg aacagcagtt
     1501 acccggcatc tatcgtgtct ctgtttacgt caatgaacag agaatggaaa cccgcacact
     1561 ggaatttaaa gaggccacag aagcacagcg caaagcgatg ggggaatccc ttgtgccttg
     1621 ccttagccgg acgcaactgg cagagatggg cgtgcgcgtt gaaagctttc cggccctcaa
     1681 tctggtgcct gccgaagcct gcgtaccgtt tgacgaaatc attccccagg cctcaagcca
     1741 tttcgacttc agtgaacaaa aactggtatt aagtttccct caggcggcga tgcatcaggt
     1801 ggcgcgtggc acggtgccgg aatctttatg ggacgaaggt atccctgcgt tgctgctgga
     1861 ttacagtttt agcgggagca acagtgaata tgactcctct ggttcttcca gcagttatga
     1921 cgatgataat ggtgccattc atcatgatga cggcgacaat acgctaaaga gcgacagcta
     1981 ctacctcaac ttacgcagcg ggctgaacct tggcgcttgg cggttacgga attacagtac
     2041 ctggagccat tccggtggta aagcgcagtg ggataatatc ggtacgtctt tgagtagggc
     2101 catcatccct ttcaaagcgc aattaacgat gggggatacg gcgaccgcgg gggatatttt
     2161 tgacagcgtt cagatgcgtg gcgcgatgtt agcctctgat gaagagatgc tgcccgacag
     2221 ccagcgaggc tttgcaccca tcgttcgagg gatcgccaaa agcaacgccg aagtcagtat
     2281 tgaacaaaac ggctacgtta tttatcgcac ctatgtgcag cctggtgcct ttgaaataaa
     2341 cgatctttac cccactgcaa acagcggtga tctcacggtc attattaaag aagcggatgg
     2401 cagtgaacaa cgctttattc agccattctc atcggtgcca attttccagc gtgaaggcca
     2461 tctcaaatat agctttgccg caggtgaata tcaggccggg aattatgaca gcgcctcgcc
     2521 gcgtttcggg cagcttgatc tgatctacgg tttaccgtgg gggatgacgg cctacggcgg
     2581 cgcgttaatc tctaataatt acaatgcgtt tgcattaggg atagggaaaa actttggtta
     2641 tatcggggcg atttccatcg atgtgacaca ggcgaaaagc gagctgaata acgatcgcga
     2701 tagccaggga caatcttatc gtttcttata ttccaagagc ttcgaaagcg gcaccgattt
     2761 ccgccttgcg ggctatcggt actctaccag cggtttctat accttccagg aagccaccga
     2821 tgtgcgcagt gacgctgaca gcgactataa ccgttatcac aagcgcagcg aaatacaggg
     2881 taacctgacg cagcaattag gggcctatgg ctctgtttat ttaaatttaa cgcagcagga
     2941 ttactggaac gacgcaggta aacagaacac ggtatcggcg ggttacaacg gacgtattgg
     3001 caaggtcagt tacagtattg cgtacagctg gagtaaaagc ccggaatggg atgaaagcga
     3061 tcgcttgtgg tctttcaata tttccgttcc gctgggccgg gcctggagta actatcgcgt
     3121 cacgaccgac caggatggtc gcaccaatca acaggttggg gtcagcggca cgctgcttga
     3181 ggatcgcaac ctgagctaca gtgttcagga aggctacgcc agcaacggtg tgggtaacag
     3241 cggtaatgct aacgttggct atcagggcgg gtccggtaat gtcaacgtag gctatagcta
     3301 cggcaaagat taccggcagc tcaactacag cgttcgcggc ggcgtgatcg ttcacagcga
     3361 aggcgtcacg ctttcccagc cgctaggcga aaccatgacg ctcatctccg tgcccggcgc
     3421 gcgcaatgct cgcgtggtga ataacggcgg cgttcaggtc gactggatgg gtaacgcggt
     3481 tgtgccttac gccacgcctt atcgtgaaaa cgaaatctca ctgcgtagcg attcgttggg
     3541 tgacgatgtt gaaaatgcat tccagaaagt ggtgccgacg cgtggggcga ttgtcagagc
     3601 gcgttttgat acccgtgttg gttaccgcgt attaatgacg ctgctccgtt ccgcgggcag
     3661 tccggtgcct ttcggggcaa cagcaacctt aatcaccgat aaacaaaatg aggtgagcag
     3721 catcgtcggt gaagaagggc agctctatat tagcgggatg ccagaggaag ggcggatact
     3781 gattaaatgg ggtaatgacg cgtcgcagca atgcgtggca ccttataaat tatccctgga
     3841 attaaaacag ggcggaatta ttcctgtttc agccaattgc cagtaagcaa ggagagcgaa
     3901 atgaaagcgg caatagcatt atccttatta ggatgcgtat tcggattttc tggcaaggca
     3961 tttgccggag atgcctgggg gccatgtacc cccgtagatg gcacgactta tcactataac
     4021 gtcgatgtgg atgtcggtgt tcccgatgca agtaaaaacg tcgcagggac ggttttaacg
     4081 gatgtcatga actggtctaa tggacaaaat gtttcgataa tttgtgaatg ccctgattca
     4141 tataaaaatg aaaaagatac tttgatacaa ggggttagtg caatagcaaa tatgaccaag
     4201 gatatcggtg gttttaagta ttattactta accgatgaac tgtctgtatc tacaaaaatt
     4261 caggtaagtt cggggcaata tggttttgtc ccctttaaag gtcaacaggc gcttcaggta
     4321 acgggctgta atgaagtcat cactactcct tatatgggcg gaggaggatt actttctttc
     4381 ttgattacta aaccctttat cggcgattca gtcattcccc ctacattaat tgccgaactg
     4441 tatgcctcca aaacaaataa agattacgga acaataccca tatcttccgt atctattcag
     4501 ggccgcgtca ccataactca ggattgcgaa ataaagcccg gcacggtgct ggatgtgcca
     4561 tttggtgaat tcccctcctc agcgtttaaa aacagacaag ggcaaatgcc tgaaggtgcg
     4621 acagagcaag aaattaacct cggctttgat tgcaacaata tctcggatgg tatcaaagtc
     4681 tcgttgcgcc ttgagggggc aaccaatgct gacgatcccc gagcggtgga tatgggcaac
     4741 cccgatattg gcgtactggt gaaagattcc agcggcaaaa ttttggtacc gaatgatgca
     4801 agcagcacca cgttattaaa tttgagcagc ctggattcta aaactcaccg caatgccgtg
     4861 attcgactgc tggctttacc gattagcacg acgggtaaag cgcctaaagg cgggacgttt
     4921 gaaggcgtaa cgaccatata tctggaaatg gagtgatgcc atgaaattca aactattgct
     4981 gcatagcggc atcgccagtt tgagtctgtt tgcctgcggg gtgaatgcgg cgacggatct
     5041 tggcccggca ggggatattc atttctccat cactatcacc actaaagctt gcgagatgga
     5101 aaaaagcgat ctcgaagtcg atatgggaac aatgacgctg caaaaacctg cggcagtcgg
     5161 tacggtgttg agcaagaaag atttcacaat tgaactcaaa gagtgcgatg ggatatccaa
     5221 agcgaccgtt gagatggaca gtcagtcgga cagcgatgat gattccatgt ttgcccttga
     5281 ggctggtggc gcaacgggtg ttgcgttgaa gatagaggac gataaaggaa cgcagcaagt
     5341 tcccaaaggc tccagcggaa cgccgattga atgggcgatt gatggcgaaa ccacgtcgct
     5401 tcactaccag gcgagttatg tggtcgtcaa cactcaggcc actggtggca cagcgaatgc
     5461 ccttgtcaat ttttccatca cctatgagta agctactggg ccagcatcct ggcccaacct
     5521 atctctgaat agtcgttaat ccgttaaaat ggtctattcc ggacttgcac ttttgtaaat
     5581 ttgtgcttca tgcacactct ttccccacac tttttccctt tgctgtggtc tacttattcg
     5641 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgctgg gcctgaaaag
     5701 tcgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
     5761 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
     5821 ggcgcggttt tttaccgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
     5881 atttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
     5941 ctttcgctgt ttggccgccg cagctggcgt attctggcat cgctggttgt gctcttttcc
     6001 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcattgct
     6061 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
     6121 tggttaatcg ccgttagcgc attgcctctt atccttatct ggaataaccg ctgtcgctac
     6181 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggc ggtcgtcgta
     6241 ctggcgggaa ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
     6301 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
     6361 ccatcaaact ggctttctgc gttggggctg tacgcctggg cgcgggtcga tgaatcttcc
     6421 gataataatt cattgcttaa cccggcgaag aaattcactt atcaggcacc gcaaaacgtt
     6481 gatgacactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggcatt
     6541 ttcggctatg agcgtaatac cacgccgaag ctggcccagg agaaaaatct ggcggcgttc
     6601 cgtggctatt cttgtgacac ggcgaccaaa ctttctctgc gttgcatgtt tgtacgtcag
     6661 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggtgctg
     6721 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
     6781 aacacgatgg cggacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaaccgt
     6841 ggcaagccgg tagatgatat gttgttggta gacgaaatgc agcaatcgct ggggcgcaac
     6901 ccggatggta agcatctgat cattctgcat accaaaggtt cgcactttaa ctacacccag
     6961 cgttacccgc gcagctttgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
     7021 accaaagcgc agatgatcaa ctcctacgac aactcggtga cctatgtgga tcactttatc
     7081 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
     7141 ggtgagtcaa ttaacgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
     7201 gagcagttcc gcgtgccgat gatggtctgg atgtcagata aatatctgga aaatccggtc
     7261 aatgcgcagg cgtttgcgca gctgaaaaaa gaagctgaca tgaaagtgcc acgccgtcac
     7321 gtagagctgt acgacaccat catgggttgt cttggctata tttcaccgga tggtggaatt
     7381 aacgaaaaca acaactggtg tcacatcccg cagacaaaag aggcagcggc taactaagag
     7441 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
     7501 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
     7561 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
     7621 ctcaacgagc aggctttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
     7681 gaggttcaac tcgacgtagc ctgataagac gcgccagcgt tgcatcaggc aaaatgtgcc
     7741 ggccattgtc ggatgcggcg tgaacgcctt attcgaccta cacgccacac atttccctgc
     7801 caaatttgcg ccccatatct cactatccat taacggattt gtgacagaaa ccattgtctt
     7861 tttttatatt tgcttaaatc tttttttgcg ttacttatgg ctggcgctgg cattttccgc
     7921 tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc caccttttcc tcagattttt
     7981 ttcactaaat gcataaaact taatcaccaa atgttgctaa atattaagct gattgttctg
     8041 gtcatgttgg agtagcacaa atttctctgc tgcaaatagc tgtttgaagt ttttttaatc
     8101 tttgtttgtg gattctcacc gttggtgtaa atcccggttg gctgtattga cgttttcaca
     8161 ttctgttgac agattgtagg tcacgagggg cattttatgg aggatccgca ctgttacact
     8221 gatgttaatt agtacggcat ccccacctca taacgttgac ccgaccgggc aaaaaacaaa
     8281 aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac aacaaacatc acaattggag
     8341 cagaataatg cgtatttcct tgaaaaagtc agggatgctg aagcttggtc tcagcctggt
     8401 ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg gtttattgct cagaaggatc
     8461 tccggaaggg tttaacccgc agctgtttac ctccggcacc acctatgacg cctcttccgt
     8521 cccgctttat aaccgtctgg ttgaatttaa aatcggcacc actgaagtga tcccgggcct
     8581 cgctgaaaag tgggaagtca gcgaagacgg taaaacctac accttccatc tgcgtaaagg
     8641 tgtgaagtgg cacgacaata aagaattcaa accgacgcgc gaactgaacg ccgatgacgt
     8701 ggtgttctcg tttgatcgtc agaaaaacgc gcaaaacccg taccataaag tatctggcgg
     8761 cagctacgaa tacttcgaag gcatgggctt gccagagctg atcagcgaag tgaaaaaggt
     8821 ggacgataac accgttcagt ttgtgctgac tcgaccggaa gcgccgttcc tcgctgacct
     8881 ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct gatgcgatga tgaaagctgg
     8941 tacaccggaa aaactggacc tcaacccaat cggtaccggt ccgttccagt tacagcagta
     9001 tcaaaaagat tcccgtattc gctacaaagc gtttgatggc tactggggca ccaaaccgca
     9061 gatcgatacg ctggtcttct ctattactcc tgacgcttcc gtgcgttacg cgaaattgca
     9121 gaagaatgaa tgccaggtga tgccgtaccc gaacccggca gatatcgctc gcatgaagca
     9181 ggataaatcc atcaatctga tggaaatgcc ggggctgaac gtcggttatc tctcgtataa
     9241 cgtgcagaaa aaaccactcg atgacgtgaa agttcgccag gctctgacct acgcggtgaa
     9301 caaagacgcg atcatcaaag cggtttatca gggcgcgggc gtatcagcga aaaacctgat
     9361 cccgccaacc atgtggggct ataacgacga cgttcaggac tacacctacg atcctgaaaa
     9421 agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt ttctccatcg acctgtgggc
     9481 gatgccggta caacgtccgt ataacccgaa cgctcgccgc atggcggaga tgattcaggc
     9541 agactgggcg aaagtgggcg tgcaggccaa aatcgtcacc tacgaatggg gtgagtacct
     9601 caagcgtgcg aaagatggcg agcatcagac ggtaatgatg ggctggactg gcgataacgg
     9661 ggacccggat aacttcttcg ccaccctgtt cagctgcgcc gcctctgaac aaggctccaa
     9721 ctactcaaaa tggtgctaca aaccgtttga agatctgatt caaccggcgc gtgctaccga
     9781 cgaccataac aaacgcgttg aactgtacaa gcaagcgcag gtggtgatgc acgatcaggc
     9841 tccggcactg atcatcgctc actccaccgt gtttgaaccg gtacgcaaag aagttaaagg
     9901 ctatgtggtt gatccattag gcaaacatca cttcgaaaac gtctctatcg aataattaaa
     9961 agccataccc ggtggcgctg tgcctctgcg tgcagcgcca tccggcagca atacttcatt
    10021 ccctgcccga cctgggcggg aattgatttg tgagcaatac agacacgcag ttccaggctg
    10081 cgggtcacta cagagaatcc gggttatgtt gcagtttatt ctccgacgtt tgggactcgt
    10141 catccccacg tttatcggta ttacccttct cacatttgcc tttgtccaca tgatcccggg
    10201 cgatccggtg atgatcatgg cgggcgaacg tgggatctcc ccagagcgtc acgcgcagct
    10261 gctggctgaa ctcggcttag ataaaccgat gtggcagcag tatctccatt acatttgggg
    10321 cgttatgcat ggcgatctag gcatttcaat gaaaagccgc atcccggtat gggaagagtt
    10381 cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc gcgatgattt ttgctacggc
    10441 agtcggtatt ccggttggtg tgctggctgc ggttaaacgc ggttccattt tcgatcacac
    10501 agcggttggc ctggcgctga caggttattc aatgcctatc ttctggtggg gcatgatgct
    10561 gatcatgctg gtttcggtgc actggaacct gacacccgtc tccggtcgcg tgagcgatat
    10621 ggtgttcctc gatgactcca atccgttaac cggttttatg ctgatcgaca ccgctatctg
    10681 gggtgaagac ggcaacttta tcgatgccgt cgcccatatg atcttgcccg ccattgtgct
    10741 gggtactatt ccgctggcgg tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct
    10801 gggcgaggat tacatccgca ccgcgcgcgc caaagggttg acccgcctta gggtgattat
    10861 cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc gttatcggcc tccaggtggg
    10921 aacattactg gcgggggcga ttctgaccga aaccatcttc tcgtggcccg gtctggggcg
    10981 ctggttgatt gacgcactgc aacgccgcga ctatccggta gtgcagggcg gcgtattgct
    11041 ggtggcgacg atgattatcc tcgtcaacct gctggtcgat ctgctgtacg gcgtggtgaa
    11101 cccgcgtatt cgtcataaga agtaagggga catcatgtca caggttactg aaaataaagt
    11161 gattagcgca ccggtgccga tgaccccgtt acaggagttc tggcactatt ttaaacgcaa
    11221 caaaggcgcg gtcgtcgggc tggtttacgt cgtcatcgtg ctgtttatcg cgatctttgc
    11281 caactggatt gcgccctata acccggctga acagttccgc gatgcactgc tcgccccgcc
    11341 agcctggcag gaaggcggca gcatggcgca cttgctgggc accgatgacg taggccgtga
    11401 tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg ctggttggct gtctggtggt
    11461 ggtgttatcg ctgattatgg gcgttattct cggcctgatc gccggttact tcggcggctt
    11521 ggtcgataac atcatcatgc gcgtggtcga tatcatgctg gcgctgccaa gtctgctgtt
    11581 ggcactggtg ctggtggcga ttttcggccc gtcgattggt aacgccgcgc tggcgctaac
    11641 cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc gccgtgctgg tggaggtgaa
    11701 tcgcgattac gttacagctt ctcgcgtggc aggtgccggg gcgatgcgcc agatgtttat
    11761 taacatcttc ccgaactgcc ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa
    11821 cgccattctc gatatggctg ctcttggttt cctcggcatg ggggcgcagc cgccaacgcc
    11881 ggagtggggc accatgctct ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt
    11941 gactttcccg ggtctggcga tcctgctgac ggtgctggca tttaacctga tgggtgacgg
    12001 tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag gttcgagatg gcgttattaa
    12061 atgtagataa attatcggtg catttcggcg acgaaagcgc accgttccgc gccgtagacc
    12121 gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat tgtgggtgag tccggctctg
    12181 gtaaatcggt cagttcgctg gcgattatgg ggctgattga ttatccgggc cgcgtaatgg
    12241 cggaaaagct ggagtttaac ggtcaggatt tacagcgtat ctcagaaaaa gagcgccgca
    12301 acctggtggg tgccgaagtg gcgatgatct tccaggaccc gatgaccagc cttaacccgt
    12361 gctacaccgt gggtttccag attatggaag cgattaaggt gcatcagggc ggcaacaaaa
    12421 gtacccgccg tcagcgggcg attgacctgc tgaatcaggt cggtattccc gatccggcat
    12481 cgcgtctgga tgtttatccg catcagcttt ccggcggcat gagccagcgc gtgatgatcg
    12541 ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga tgaaccaacc actgcgctgg
    12601 acgtgaccat tcaggcgcaa atcattgaac tactgcttga gctacagcag aaagagaaca
    12661 tggcgctggt gttaattacc catgacctgg cgctggtggc ggaagctgcg cataaaatca
    12721 ttgtgatgta tgccggtcaa gtggtggaaa cgggcgatgc tcacgccatc ttccatgcgc
    12781 cgcgtcatcc gtatactcag gcattgctgc gtgcgctgcc ggaatttgct caggacaaag
    12841 aacgtctggc atcgttgcct ggcgttgttc ccggcaagta cgaccgcccg aacggctgcc
    12901 tgcttaaccc gcgctgcccc tatgccactg acagatgtcg cgctgaagaa ccggcgctga
    12961 atatgctcgc tgacgggcgt cagtccaaat gccattaccc acttgatgat gccgggaggc
    13021 cgacactatg agtacgcaag aggccacctc gcaacaaccg ctgttgcagg ctatcgacct
    13081 gaaaaaacat tatccggtga agaaaggtat gttcgctccg gaacgtctgg tgaaggcgct
    13141 ggacggcgtt tcgtttaacc ttgaacgtgg caaaacgctg gcggtagtag gtgaatctgg
    13201 ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt gaaacgccca ccggcggcga
    13261 gctgtattac caggggcagg atctgcttaa gcacgatccg caggcgcaga agctgcgtcg
    13321 gcagaaaatc cagattgtct tccagaaccc ttacggttcg ctgaatccgc gtaaaaaagt
    13381 cgggcaaatt cttgaagagc cgctgctgat caacaccagt ttaagcaaag aacagcgtcg
    13441 ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa accgagcact atgaccgcta
    13501 tccgcatatg ttctccggcg gacaacgtca gcgtatcgct attgcccgtg gtctgatgct
    13561 cgacccggac gtggtgattg ccgatgaacc ggtctccgcg ctggacgtct cggtgcgtgc
    13621 acaggtgctc aacctgatga tggatttgca gcaggaactg gggctttctt atgtcttcat
    13681 ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa gtgatggtga tgtacctggg
    13741 ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat aacccgcgcc atccgtacac
    13801 tcaggcgcta ctttccgcga cgccgcgcct gaatccggac gatcgccgcg agcgcatcaa
    13861 actcaccggt gaactgccaa gcccgctgaa tccaccgccg ggctgcgcgt ttaacgcccg
    13921 ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg cagctaaaag actacggcgg
    13981 tcaactggta gcttgttttg ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa
    14041 accggggcca tgcgctccgg tttactcaat cagcttcaac agtggcgaca agcatagcaa
    14101 aacgccgtaa agcagaatca gccaggcttt aaatccgcga agtttttcca gttgtgcgac
    14161 tttatagatc aggaaaaacg gaatgaggca cgacacaatg ccatataacg ggcttcccaa
    14221 ctgaaagaac accagcaccg atacacgaaa cgaaacccaa atcgtcaacg taatgacgat
    14281 aaaagtgcag atcgccagag tcagcacgcg tgagttaatt ttcttggtat caataattcg
    14341 gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa
    14401 gaatgcggtc agtacggcaa agatattcag caccgtagag gtgatatgaa tgatatgccc
    14461 agggatcacc tgcgcggcca gtgccagtgc cgagatattt tgttcaaagg cagaaacggc
    14521 ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa aacagtatca ccgcgatgag
    14581 cgtgatataa ctaattcggt gggtacgcag cgcgagccgg gttgccagta ctttatccgc
    14641 ttcccgttta cgataggcaa tattcattgg gttaagtacc tgaataaata ctgcagaaaa
    14701 gaagcaaaat ggaatggtaa gcaggacatc gcgaaaaaag actgaggctt gcgggaaggc
    14761 ggttatattg gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga caataatccc
    14821 tactttgacc accaccattg gcccagaaat cttaaacagt aatcgttcac caccagacgc
    14881 aatcgctacc agtacggcga aaatagcgac tttataaagt agagattgtg aaagatcggc
    14941 atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg agagagagta
    15001 gataaaaatc ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt
    15061 cttccccagg taatgactga taatatcggt gtagtcatta caggaatcgc tttcagaaag
    15121 ggtttttaaa taaatgtcct gcactaccca ggtggcagga taagcaatga tcgctgcggt
    15181 aataaatacc caaattccct tcaagccaat ttgtactggc atcagcacgg ttccggcacc
    15241 aatcgccatg cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt
    15301 ctgatcgtgt ttcggtagtg tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc
    15361 tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg tggagaagtc gaatacgcag
    15421 tatgtaaaaa atgaggcaac agattcactg gggaggtcgc gaggaggcgc aattctacaa
    15481 aattgcgcat ttcactacca gtctggagtc agcccggata tgaactgcaa ctattgcaaa
    15541 ttaagaaatt aagctggaag gcatcacgca atgaggaaga ttgccgagtg ctgacagtaa
    15601 aaagcaatcg taaaattagc ggcaagccgg aagggttccg gtgaggcgca atgttgcggg
    15661 ggcttaatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat
    15721 gcacctgaca acaccacggg ggccattctt gcatccaaac aatacaagaa tagccgcgaa
    15781 acgttgtcat tacaatacag gcggctatat gacgctcgca gagctggaca tggccttctg
    15841 gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct
    15901 gaacaaacgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc
    15961 ggaccaggcc acaggggtga aactctgcgg cctttttcgt gcttactgcg ggtaaggcac
    16021 ccagtcgccg ccgttcagtc gaacgtacgg tttatcctgg tattgaataa ctactgcatt
    16081 tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt tccagtcaac
    16141 attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg agatagccag
    16201 gaagctgctc ggttggtcga tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa
    16261 gaatttcacc ccaacgggga cgtcggtgat agacgggcta gggatatcac gtaggccaga
    16321 tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac
    16381 tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg
    16441 cgcccgcgct ttgtaatccg ctgttttgct gacgcccgga taatggttgc cgtcatgcag
    16501 tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag
    16561 ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat
    16621 aaccggcaga tttgtttgat ccatcaattc gctctgcatg ccgccatttt cgcgaacttc
    16681 tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag
    16741 tttcgacagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg
    16801 cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgct gccgggccac tgtaggaggt
    16861 ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagcggat gcgacatcaa
    16921 cccggcggct tcaatatccg accaggaaag cgaacagata ttaatcacca gtagttcaaa
    16981 tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg cctccgcgtt
    17041 atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg
    17101 catatcaccc actaccggtg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc
    17161 cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac ttggtcccgc
    17221 cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca
    17281 ttgtgacagg aataaccaag ccactaataa aacaaaaatg gccccaatca tctgccagtt
    17341 aataaagcgg gtgacaaggt cgattaaata atcggtactg aaccccgcca cctgcgaacc
    17401 ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc
    17461 gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa tgggcatcag
    17521 cagaaacgcg gcaaacacca aattgaggag cggatggaag ttaagatatc ccgcccacaa
    17581 caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca
    17641 gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct
    17701 ttttccgtgc ggcgtaacgt cccagccggt ttaacataac gaggtttagc aaagaacaaa
    17761 cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat
    17821 atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat gatgagcgct
    17881 ccacagcatc atctaatagt cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt
    17941 gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg ccccattgtt
    18001 ctggggcaag caggcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga
    18061 ccatatggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat
    18121 ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca
    18181 tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg cggatacccg
    18241 gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga gggttattca
    18301 tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc agtttgagcg
    18361 gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta aacttctgcc
    18421 cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac ggaataacca
    18481 tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta gcggtatttt
    18541 cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga atgctgcggg
    18601 ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcga
    18661 tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga ttttaatcga
    18721 cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg atcactcgct
    18781 ggcaatcgtc aatgtggatg ccaactgcca tatccgacta catcagcaag cgctgccgga
    18841 tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg acgatattta
    18901 ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc gtgatgaagc
    18961 gatggctgaa tgcctggcgg ctaagcaacc agtaggtgaa tatcgcagtg atgcgctggc
    19021 ggctgaagag atactgacgc tggcgaactg gtgcctgttg aactattccg ggctgaaaac
    19081 gccagtcggg agtaaatcat gagtatcctg acccggtggt tgctaatccc gccggtcaac
    19141 gcgcggctta tcgggcgtta tcgcgattat cgtcgccacg gtgcgtcggc tttcagcgcg
    19201 acgctcggct gtttttggtt gatcctggcc tggattttta ttccgctgga gcacccgcgc
    19261 tggcagcgta ttcgcgcaga acataaaaac ctgtatccgc atatcaacgc ctcgcgtccg
    19321 cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttactgat cggtgcatca
    19381 cgcaaagaaa cgccgaaacc gcgcaggcgg gcattttcag gtctgcagaa tattcgtgga
    19441 cgttaccatc aatggatgaa cgagctgcct gagcgcgtta gccataaaac acagcatctt
    19501 gatgagaaaa aagagctcgg tcatttgagt gccggggcgc ggcggttgat cctcggtatc
    19561 atcgtcacct tctcgctgat tctggcgtta atctgcgtta ctcagccgtt taacccgctg
    19621 gcgcagttta tcttcctgat gctgctgtgg ggggtagcgc tgatcgtacg gcggatgccg
    19681 gggcgcttct cagcgctaat gttgattgtg ctgtcgctga ctgtttcttg ccgttatatc
    19741 tggtggcgat atacctctac gctgaactgg gacgatccgg tcagcctggt gtgcgggctt
    19801 attctgctct tcgctgaaac gtacgcgtgg attgtgctgg tgctcggcta cttccaggta
    19861 gtatggccgc tgaatcgtca gccggtgcca ttgccgaaag atatgtcgct gtggccgtcg
    19921 gtggatatct ttgtcccgac ttacaacgaa gatctcaacg tggtgaaaaa taccatttac
    19981 gcctcgctgg gtatcgactg gccgaaagac aaactgaaca tctggatcct cgatgacggc
    20041 ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg tgaaatatat cgcccgcacc
    20101 actcatgaac atgcgaaagc gggcaacatc aacaatgcgc tgaaatatgc caaaggcgag
    20161 ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat cgttcctgca aatgaccatg
    20221 ggctggttcc tgaaagagaa acagctggcg atgatgcaga cgccgcacca cttcttctca
    20281 ccggacccgt ttgaacgcaa cctggggcgt tttcgtaaaa cgccgaacga aggcacgctg
    20341 ttctatggtc tggtgcagga tggcaacgat atgtgggacg ccactttctt ctgcggatcc
    20401 tgtgcggtga ttcgccgtaa gccgctggat gaaattggcg gcattgctgt cgaaaccgtg
    20461 actgaagatg cgcatacttc tttgcgcctg caccgtcgtg gctacacctc tgcatatatg
    20521 cgtattccgc aggcggcggg gctggcgacc gaaagtctgt cggcacatat cggtcagcgt
    20581 attcgctggg cgcgcgggat ggtacaaatc ttccgtctcg ataacccgct caccggtaaa
    20641 gggctgaagt ttgctcagcg gctatgttac gtcaacgcca tgttccactt cttgtcgggc
    20701 attccacggc tgatcttcct gactgcgccg ctggcgttcc tgctgcttca tgcctacatc
    20761 atctatgcgc cagcgttgat gatcgccctg ttcgtgctgc cgcatatgat ccatgccagc
    20821 ctgaccaact ccaagatcca gggcaaatat cgccactctt tctggagtga aatctacgaa
    20881 acggtgctgg cgtggtatat cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa
    20941 ggcaaattta acgtcaccgc caaaggtgga ctggtggaag aagagtacgt cgactgggtg
    21001 atctcgcggc cctacatctt ccttgttctg ctcaacctgg tgggcgttgc ggtaggtatc
    21061 tggcgctact tctatggccc gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg
    21121 gtattctaca acctgattgt tcttggcggc gcagttgcgg tatcggtaga aagcaaacag
    21181 gtacgccgat cgcaccgcgt ggagatgacg atgcccgcgg caattgcccg cgaagatggt
    21241 cacctctttt cgtgtaccgt tcaggatttc tccgacggtg gtttggggat caagatcaac
    21301 ggtcaggcgc agattctgga agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag
    21361 gaatacgttt tcccgaccca agtggcgcgc gtgatgggta atgaagttgg gctgaaatta
    21421 atgccgctca ccacccagca acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat
    21481 acatgggcgc tctggcagga cagctatccg gaagataagc cgctggaaag tctgctggat
    21541 attctgaagc tcggcttccg tggctaccgc catctggcgg agtttgcgcc ttcttcggtg
    21601 aagggcatat tccgtgtgct gacttctctg gtttcctggg ttgtatcgtt tattccgcgc
    21661 cgcccggagc ggagcgaaac ggcacaacca tcggatcagg ctttggctca acaatgatga
    21721 taacgcgatg aaaagaaaac tattctggat ttgtgcagtg gctatgggga tgagtgcgtt
    21781 cccctctttc atgacgcagg cgacgccagc aacgcaacca ctgatcaatg ctgagccagc
    21841 tgtagccgcc cagacggaac aaaatccgca ggtggggcaa gtgatgccgg gcgtgcaggg
    21901 cgctgatgcg ccagtcgtgg cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc
    21961 gcaaattgcg ccgccgccgg gcagcatggt gctacgtggc attaacccga acggcagcat
    22021 tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg atgctcaacc tcgaatacac
    22081 gccatcgcca tcgttactgc ctgtccagtc gcagttaaag gtttatctca atgatgagct
    22141 gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc
    22201 cattaatcca ctgtttatta ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta
    22261 tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg ctggatgttg ggcggagcag
    22321 tggactggat ctgacctatc agaccctgaa tgtgaagaat gacctgtcac acttcccggt
    22381 gccattcttt gacccgcgtg ataaccgcac caacaccttg ccgatggtct ttgcgggtgc
    22441 gccggatgtt gggctgcaac aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc
    22501 tggctggcgt gggcagaact tcccggtgct ctataaccaa ctgccggatc gtaatgccat
    22561 tgtctttgca accaacgaca aacggccgga cttcctgcgc gatcatccgg cggtaaaagc
    22621 cccggtgatt gagatgatta accatccgca gaatccttac gtcaaactgc tggtggtgtt
    22681 tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt atcgctcagg gtaacattct
    22741 gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta
    22801 cgatgcgccg aactgggtgc gtaccgatcg tccggtcacc tttggtgaac tgaaaaccta
    22861 tgaagaacag ttacaatcca gcggtcttga gccagcagcg attaatgttt cgctaaacct
    22921 gccgccagat ctctacctga tgcgcagtac cggcattgat atggatatta attaccgcta
    22981 caccatgccg ccggtgaaag acagttcgcg gatggatatc agcctgaata accagttcct
    23041 gcaatccttc aacctgagca gcaaacagga ggcgaatcgc ctgctgctgc ggattccggt
    23101 attacaaggt ttgctggatg gcaaaacgga tgtctctatt ccggcgctga aactgggcgc
    23161 gaccaaccag ctgcgcttcg actttgagta tatgaacctg atgcctggcg gttcggtgga
    23221 taactgtatt accttccagc cagtgcagaa tcatgtggtg attggtgacg actccaccat
    23281 cgacttctcg aagtattacc acttcatccc gatgccggat ctacgcgcct ttgctaacgc
    23341 gggcttccca ttcagccgga tggcggatct gtcgcaaacc atcaccgtga tgccgaaagc
    23401 gcctaacgaa gcacagatgg aaacgttgct gaatactgtt ggttttatcg gcgcacagac
    23461 gggcttcccg gcgattaatc tgacggtgac cgatgatggc agcaccattc agggcaaaga
    23521 tgccgacatc atgatcatcg gtggtatccc ggacaaactg aaagacgata agcagatcga
    23581 cctgttggtg caggcgaccg aaagctgggt gaaaacaccg atgcgccaga ccccgttccc
    23641 cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc cagtcaacgc tgacctcttc
    23701 cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat
    23761 tgcgctgctg gcagatagcc cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag
    23821 cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag
    23881 cctacgtgtt ggcgacgttt attacgtagg tcatctgccg tggttcgagc gcttgtggta
    23941 tgcgctggca aaccatccga ttcttctggc ggtgctggcg gctatcagtg tgatattgct
    24001 ggcatgggta ctgtggcgtc tgctgcgaat tattagtcgt cgtcgtctta acccggataa
    24061 cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg acgatgctgc tgttggctgc
    24121 ctttagtgtt caggcagcct gtacctggcc tgcctgggag cagtttaaaa aggattacat
    24181 cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc aaaatcacca cctctgaagg
    24241 gcaaagttac ggcatgttct ttgccctggc ggctaacgac cgtgcagctt tcgataatat
    24301 tctcgactgg acgcagaaca atctcgctca gggttcttta aaagaacgtt tgcccgcctg
    24361 gctgtggggc aagaaagaga acagtaagtg ggaagtgctg gacagcaatt cggcctccga
    24421 tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg
    24481 ttataccgac atcggcagcg cgttgctaaa acgtattgcg cgggaggaag tggtgacggt
    24541 gcctgggctg ggttccatgt tgttaccggg caaagtgggt tttgcagagg ataacagctg
    24601 gcgttttaac cccagctacc tgccgccgac gctggcgcag tatttcaccc gctttggcgc
    24661 gccgtggacc acgctgcgcg aaaccaatca acgtttattg ctggaaaccg ccccgaaagg
    24721 cttttcgcca gactgggtgc gctatgagaa agacaaaggc tggcagctaa aagccgaaaa
    24781 aacattgatc agcagctacg acgctatccg cgtttacatg tgggtaggca tgatgcctga
    24841 cagcgatccg cagaaagcgc ggatgctcaa ccggtttaaa ccgatggcga cattcactga
    24901 gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg gggaaagggc agggtaaagg
    24961 accagtcggt ttttctgccg ccatgctgcc ctttttacaa aaccgcgatg cgcaggccgt
    25021 tcagcgccag cgcgtggccg ataactttcc cggcagcgat gcctattaca actatgtgct
    25081 gaccctgttt ggacaaggct gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt
    25141 attacctgac tggggccagg aatgcgcaaa ttcacactaa acatattcac gctttccctc
    25201 ggtctggccg tcatgccgat ggtcgaggca gcaccaaccg ctcagcaaca gttgctggag
    25261 caagttcggt taggcgaagc gacccatcgt gaagacctgg tacagcagtc gttgtatcgg
    25321 ctggaactta ttgatccgaa taacccggac gtcgttgccg cccgtttccg ttctttgtta
    25381 cgtcagggcg atattgatgg cgcgcaaaaa cagctcgatc ggctgtcgca gttagcgccg
    25441 agttcaaatg cgtataaatc gtcgcggact acgatgctac tttccacgcc ggatggtcgt
    25501 caggcactgc aacaggcaag attgcaggcg actaccggtc atgcggaaga agctgtggcg
    25561 agttacaaca aactgttcaa cggtgcgccg ccggaaggtg acattgctgt cgagtactgg
    25621 agtacggtgg cgaaaattcc ggctcgccgt ggcgaagcga ttaatcagtt aaaacgcatc
    25681 aatgcggatg caccgggcaa tacgggcctg caaaacaatc tggcgctatt gctgtttagt
    25741 agcgatcgcc gtgacgaagg ttttgccgtc ctggaacaga tggcgaaatc gaacgccggg
    25801 cgcgaagggg cctctaaaat ctggtacggg cagattaaag acatgcccgt cagtgatgcc
    25861 agtgtgtcgg cgctgaaaaa atatctttcg atctttagtg atggcgatag cgtggcggct
    25921 gcgcaatcgc aactggcaga acagaaaaaa cagctggccg atcctgcttt ccgcgctcgt
    25981 gcgcaaggtt tagcggcggt ggactctggt atggcgggta aagccattcc cgaactacaa
    26041 caggcagtgc gggcgaaccc gaaagacagt gaggctctgg gggcgctggg ccaggcatat
    26101 tcacagaaag gcgatcgcgc caatgcagtg gcgaatctgg aaaaagccct cgcactggac
    26161 ccgcacagca gcaacaacga caaatggaac agtctgctga aagtaaaccg ctactggctg
    26221 gcaatccagc agggcgatgc tgcgctgaaa gccaataatc ctgaccgggc agaacgcctg
    26281 ttccagcagg cgcgtaatgt cgataacacc gacagttatg cagtgctggg gctgggcgat
    26341 gtggcgatgg cgcgaaaaga ttatcccgcc gccgaacgtt attatcagca gaccttgcgt
    26401 atggacagcg gcaacactaa cgccgtgcgc gggctggcaa atatttaccg ccagcaatcg
    26461 ccagaaaaag ctgaagcgtt tatcgcctca ctctctgcca gtcagcggcg tagcattgat
    26521 gatatcgaac gcagcctgca aaacgaccgt ctggcacagc aggcagaggc actggaaaac
    26581 cagggcaaat gggcgcaggc ggcagcactt cagcggcaac gactggcgct ggaccccggc
    26641 agcatatgga ttacttaccg actttcgcag gatctctggc aggccggaca acgcagccag
    26701 gccgatacgt taatgcgcaa tctggcgcag cagaagccga acgacccgga gcaggtttac
    26761 gcttacgggc tgtacctctc tggtcatgac caggacagag cggcgctggc gcatatcaac
    26821 agcctgccgc gtgcgcagtg gagcagcaat attcaggagc tggttaatcg actgcaaagc
    26881 gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg gcaaagaggc agaagcggaa
    26941 gcgatgctgc accagcaacc gccttcctcg cgtattgacc tctcgctggc tgactgggcg
    27001 caacaacgac gtgattacac cgccgcccgc gctgcgtatc agaatgtcct gacgcgggag
    27061 ccaactaacg ccgatgccat tcttggtctg acggaagtgg atattgctgc cggtgacaaa
    27121 gcggcggcac gtagccagct ggcgaaactg cccgctaccg ataacgcctc gctgaacaca
    27181 cagcggcgcg tggcgctggc acaggcgcag cttggcgata ccgcagtggc gcagcagacg
    27241 tttaataagt tgatcccgca ggcaaaatct cagccaccgt cgatggaaag cgcgatggtg
    27301 ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc caaagcaggc gctggaaacc
    27361 tacaaagacg ccatggtcgc atccggtgtg actacgacgc gtccgcagga taacgacacc
    27421 tttacccgac tgacccgtaa cgacgagaaa gatgactggc tgaaacgcgg cgtgcgcagc
    27481 gatgcggcgg acctctatcg ccagcaggat cttaacgtca cccttgagca tgattattgg
    27541 ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc acactaccat gttgcaggtg
    27601 gatgcgccgt attctgacgg gcggatgttc tttcgcagtg atttcgtcaa tatgaacgtc
    27661 ggcagtttct ccactaatgc cgatggtaaa tgggatgata actggggcac ctgtacatta
    27721 caggactgta gcggcaaccg cagccagtcg gattccggtg ccagcgtggc ggtcggctgg
    27781 cgaaatgacg tctggagctg ggatatcggc accacgccga tgggcttcaa cgtggtggat
    27841 gtggtcggcg gcatcagtta cagcgatgat atcgggccgc tgggttacac cgttaacgcc
    27901 caccgtcggc ccatctccag ttctttgctg gcctttggtg ggcaaaaaga ctccccgagc
    27961 aataccggga aaaaatgggg cggtgtgcgt gccgacggcg tggggctaag cctgagctac
    28021 gataaaggtg aagcaaacgg cgtctgggca tcgcttagtg gcgaccagtt aaccggtaaa
    28081 aatgtcgaag ataactggcg cgtgcgctgg atgacgggct attactataa ggtcatcaac
    28141 cagaacaatc gccgcgtcac aatcggcctg aacaacatga tctggcatta cgacaaagac
    28201 ctgagtggct actcactcgg tcagggcggt tactacagcc cgcaggaata cctgtcgttt
    28261 gccataccgg tgatgtggcg ggagcgcacg gaaaactggt cgtgggagct gggggcgtct
    28321 ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt atccgctgat gaatctgatc
    28381 ccgactgact ggcaggaaga agctgcgcgg caatccaacg atggcggcag cagtcagggc
    28441 tttggctata cggcgcgggc attacttgag cgtcgcgtca catccaactg gtttgtcggt
    28501 acggcaattg atatccagca ggcgaaagat tacgccccca gccatttcct gctctacgta
    28561 cgttattccg ccgccggatg gcagggtgat atggatttac cgccgcagcc gctgatacct
    28621 tacgccgact ggtaagtttt cagatagcgc ctctcttaat gccgctgcga tcgggtatac
    28681 tcgggcggca atctgggatt tccgggggga gacaatttgc gcgtaagtcg ctcgttaaca
    28741 atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt tcgtttttat tttttgcacc
    28801 gttttgctgt tccatctggt ccagcagaat cgctataaca cggctacgca actggaaagc
    28861 attgctcgct ctgtccgcga acctttatct tcagccattt tgaaaggcga tattcccgaa
    28921 gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg tcagccgtgc cgatgtagtg
    28981 ctgcccaacc agttccaggc gctgcgtaaa agttttattc cagagcgccc ggtgccggta
    29041 atggttactc gcctgtttga gctaccggtt caaatctcgc tgggcgtcta ctcgctcgaa
    29101 cgtccggcaa acccgcagcc aattgcctat ctggtactac aggcggattc cttccgtatg
    29161 tataagttcg tgatgagcac tctctcaacg ttagtgacca tttacttact tttgtcgtta
    29221 atattgacgg tggcgattag ctggtgcatt aaccgcctga ttttgcatcc gttacgcaat
    29281 attgctcgcg aacttaacgc catcccagcc caggagcttg ttggtcacca actggcatta
    29341 ccgcgtctgc atcaggatga tgaaatcggt atgttggtgc gcagttacaa cctcaaccag
    29401 caattgctgc agcgccatta tgaagaacag aacgaaaatg cgatgcgctt cccggtgtcg
    29461 gatttgccga acaaagcctt gctgatggag atgctggagc aggttgtcgc gcgtaaacaa
    29521 accaccgcgc tgatgatcat cacctgtgaa accctgcgtg atactgcggg tgtgctgcaa
    29581 gaggcgcaac gagaaattct gctgctgacg ctggtggaaa aactcaaatc ggtactgtcg
    29641 ccacgtatga tcctcgcgca gattagcggt tatgactttg ctgtcattgc caatggtgta
    29701 caggaaccgt ggcacgcaat caccttaggt cagcaagtgc tcactatcat gagcgagcgc
    29761 ctgccgattg aacgtattca actccgtccg cactgtagca ttggcgtggc gatgttctac
    29821 ggcgatctca ccgccgaaca gctttacagt cgcgctattt ctgcggcatt taccgctcgc
    29881 cataaaggca agaatcagat tcagttcttt gatccgcagc agatggaagc tgcccagaag
    29941 cggttgacgg aagagagcga tatccttaat gcattggaaa atcatcagtt tgctatttgg
    30001 ttacagccac aggtcgagat gaccagcggt aaactggtca gtgcggaagt gttactgcgt
    30061 atccagcaac cggatggcag ttgggacctg ccggatggct taatcgatcg cattgagtgc
    30121 tgtgggctga tggttaccgt cggtcactgg gtgctggaag agtcctgtcg actgttggct
    30181 gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa acctctcagc gctgcaactg
    30241 atgcacccaa atatggtggc ggatatgctg gaactgttaa cccgctatcg cattcagccg
    30301 ggaacactga ttctggaagt gacagaaagc cgacgtattg acgaccctca tgctgcggtg
    30361 gcaatcctcc gtccgctgcg taatgccgga gttcgggtgg cgctggatga tttcggcatg
    30421 ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt tgccaatcga cgttctgaaa
    30481 atcgacaaaa tgtttgttga aggcttgccg gaagatagca gcatgattgc tgcaattatc
    30541 atgctggcgc agagcctgaa cttacaaatg attgccgaag gcgtggagac tgaagcacaa
    30601 cgcgactggc tggcaaaagc gggcgttggt attgcccagg gcttcctttt tgctcgtcca
    30661 ctccctattg aaatcttcga agagagttac ctggaagaaa agtagctacc ccaaactgat
    30721 tacaaaactt taaaaagtgc tggtttgtgc gagccagctc aaacttttta acctttttgt
    30781 ttcaattatg atccaggtac atttctgtga tgttgtctgg gtgttatttt aaggccgcag
    30841 gtaccccata accttacaag acctgtggtt ttactaaagg acaccctatg aaaacctctc
    30901 tgtttaaaag cctttacttt caggtcctga cagcgatagc cattggtatt ctccttggcc
    30961 atttctatcc tgaaataggc gagcaaatga aaccgcttgg cgacggcttc gttaagctca
    31021 ttaagatgat catcgctcct gtcatctttt gtaccgtcgt aacgggcatt gcgggcatgg
    31081 aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact gctttacttt gaaattgtca
    31141 gtaccatcgc gctgattatt ggtcttatca tcgttaacgt cgtgcagcct ggtgccggaa
    31201 tgaacgtcga tccggcaacg cttgatgcga aagcggtagc ggtttacgcc gatcaggcga
    31261 aagaccaggg cattgtcgcc ttcattatgg atgtcatccc ggcgagcgtc attggcgcat
    31321 ttgccagcgg taacattctg caggtgctgc tgtttgccgt actgtttggt tttgcgctcc
    31381 accgtctggg cagcaaaggc caactgattt ttaacgtcat cgaaagtttc tcgcaggtca
    31441 tcttcggcat catcaatatg atcatgcgtc tggcacctat tggtgcgttc ggggcaatgg
    31501 cgtttaccat cggtaaatac ggcgtcggca cactggtgca actggggcag ctgattatct
    31561 gtttctacat tacctgtatc ctgtttgtgg tgctggtatt gggttcaatc gctaaagcga
    31621 ctggtttcag tatcttcaaa tttatccgct acatccgtga agaactgctg attgtactgg
    31681 ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga caagatggag aaactcggct
    31741 gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta ctcgtttaac cttgatggca
    31801 catcgatata cctgacaatg gcggcggtgt ttatcgccca ggccactaac agccagatgg
    31861 atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct ttcttctaaa ggggcggcag
    31921 gggtaacggg tagtggcttt atcgtgctgg cggcgacgct ctctgcggtg ggccatttgc
    31981 cggtagcggg tctggcgctg atcctcggta tcgaccgctt tatgtcagaa gctcgtgcgc
    32041 tgactaacct ggtcggtaac ggcgtagcga ccattgtcgt tgctaagtgg gtgaaagaac
    32101 tggaccacaa aaaactggac gatgtgctga ataatcgtgc gccggatggc aaaacgcacg
    32161 aattatcctc ttaatctcat cacttatgcc cgcactccct tccccgagtg cgggtgtttg
    32221 cgcataattg acccctgtgc ctgaacttac cacctgggtg agttcaggtt tatttcactt
    32281 cttccgtgac attttcatgt tcttgcggtc taacacgaag tgtttttaac gtcatttaga
    32341 ctgaccgaca acgggcagtt ttttattacc aggatagttg atcaggggtt cacatgcagg
    32401 gcacaaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct ggctatgtgc
    32461 aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc aacggtttac
    32521 agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt cgcctgctgg
    32581 ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac gccatccctc
    32641 gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca ttgtggcagc
    32701 aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac accacgctgt
    32761 ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaggcgctc tcttatctgg
    32821 caaatgccac tggcaaactg actatcacgc cagaaaccat caaccacgcg ctgcaaagtc
    32881 aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc tatcgtctga
    32941 aaggatcaac cctgttaggt cacgatcctg ccgatccgct gaaacaaccc gttgaagcgg
    33001 aaaagattaa agatttctat cagaaatggt acaccccgga tgcaatgacg ctgctggtgg
    33061 tgggaaacgt ggatgcgcgc tcggtcgtcg accaaatcaa caaaacgttt ggcgaactga
    33121 aaggcaaacg tgaaacaccg gctccggtgc cgacgctttc tccgctgcgt gcggaagcgg
    33181 tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg gatacgccgt
    33241 ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac ctggcccgtg
    33301 aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag taacagcaaa gacatcggtc
    33361 ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac atcgaatcac
    33421 caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg aaggttcgcg
    33481 ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa ctggagctgc
    33541 agaaactgtt tgccgcctat gcacgagctg ataccgatat tctgatgggt cagcggatgc
    33601 gttcgttgca aaatcaggtc gtcgatatcg cgccggagca ctatcagaaa ctgcggcagg
    33661 atttccttaa cagcctgact gtggagatgt taaatcagga tctgcgtcag cagttgtcga
    33721 atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac atgaaagcgt
    33781 tgcaggcggc ctgggatcaa atcatggccc catctaccgc cgctgcgacc acctctgtcg
    33841 ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa tgcctgatgg
    33901 tgatgagtta gcacagccat caggtattta tggatgagct ggatagaggg gtaactcttt
    33961 atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg ataatcgcgc
    34021 cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgctgcg ttttccgcac
    34081 tgccgcccgt caaacgtacc gccagataac cggcactgaa agagtcgcca gctgcggtgg
    34141 tatcgatcac tttttctttc ggcagtttca ccgccggaac atccactaac ccttcgccag
    34201 caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc acgcccgcgt
    34261 tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg tcttcatcgt
    34321 ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ttggtacacc tgctgtgtct
    34381 cttctttgct ggcccacagg cgtggacgat agttattgtc gaaaatcact tttccgccgt
    34441 tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg cttaagatcg
    34501 ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg caaatcgccg
    34561 cggattgatc gctctccagc cagaatttgg cggcggcttc gttacgccag tagtagaacg
    34621 tacgttcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggcagacgg ttttccatcc
    34681 gttgggtcag ggaagtatca acgttctcgc cgtgccaggc gtccagcatc tgctggctaa
    34741 aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga tcgacctgac
    34801 gggcgatata gacggaagtg ttcagggtat cgccgccgaa acagcgctta acgtccgcgc
    34861 ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg gacatagtcg
    34921 tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc tggtcaacca
    34981 tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa gattccgttg
    35041 tggagtgggg aaatttaccc cggccaggcg gtaaagttct gcccttgcgc gccgataatc
    35101 tttgtcgagt ccgggctgca tcacttttaa acacaggaca tctttgatga taaggcaggt
    35161 tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac ggcgtttttg
    35221 gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg ggcggttaat
    35281 ggccgtggag ctattaacgg cggtcacgca tcccttgaac ccttcgcaac gcctgccgcc
    35341 ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga aagagcagat
    35401 tgatttgctg gcgcaaaaag ccgacttctt tatagagcac ggcctgctgg catcggtcaa
    35461 tattgatggt cctacgctca tcgccctgcg tcagcaacca aaaatcctgc gccagattga
    35521 gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc cgtctgccga aagattcaac
    35581 ctttgcctcg atgtgtgaat ttggcccgct gtggctggat gattttggta ccgggatggc
    35641 aaatttctct gcactaagtg aagtgcgtta tgactacatc aaaatcgcgc gagaactgtt
    35701 tgtgatgctg cgtcagtcgc cggaaggacg cacactcttt tctcagcttt tacatctaat
    35761 gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa acgccggaag agtggcgtga
    35821 tgtacagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc cggcaccgat
    35881 agaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg cctggctgga
    35941 ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca aggcaggcaa
    36001 aataaccgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga tcattttgat
    36061 tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct ctgcggagtt
    36121 gaatcgtccg ttcgctatcc gtggcgatct gggcgtggtg tgggagcggc aaaagcaaga
    36181 aactggctgg cgcagctggg tgccgtggcc ccatgtacac gcggaagaca tcattcttgg
    36241 caatccaccg gatattcccg aagtcacgat ggtgcatttg ccacgcgtag aggcaacgct
    36301 ggccccgctg gcgctgctga ccaaaacggt ctggctgccg tggatcaagc tcgaaaagcc
    36361 cgacgcgcgc ctgattcgcc tctctgaaaa gaacaataac tggacgttta atcttgccaa
    36421 cgatgacaac aaaaacgcga atgcaaagcc gtcggcgtgg tcgtttcggc tggataatat
    36481 tctgttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg atctggagat
    36541 ttttgttgat cccttaggca agccgctgcc gttcagcgaa tttactggat cgaaaggtaa
    36601 agcggataaa gaaaaggtgg gcgattacgt ttttggcctg aaggcgcagg gacgttataa
    36661 cggtgaaccg ctcacgggta aggggaaaat tggcggtatg ctggcgctgc gtggcgaagg
    36721 gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg cttttgatgg
    36781 cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat tttctggcga
    36841 ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc cgccgtttga
    36901 aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct ttgattatcg
    36961 cggctttaat gggcgaattg gcgatagcga tatccacggt tctctggtct acaccaccgg
    37021 caagccacga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc tggcggacct
    37081 gggaccgttg attggcgttg attccgggaa aggggcagaa aagtcgaaac ggtctgaaca
    37141 gaagaagggc gaaaaaagcg ttcaacctgc gggtaaagtg ctgccttatg accgcttcga
    37201 aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaaggccgac gtatcgagca
    37261 tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa atgctgacct
    37321 gcgcctgcaa ccgctgaaat ttggcatggc gggtggcagc attgcggcga atattcatct
    37381 ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc gactgaaact
    37441 gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa tgaacggtga
    37501 cgcggaactg cgcggtagcg gtaactcggt ggcggcactt ttaggcaaca gtaacggcaa
    37561 cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga ttgttgggct
    37621 gaatgtcggc aactacattg tcggtgcgat atttggtgat gatgaggtgc gggtgaactg
    37681 cgcggcggcg aatctgaata ttgccaacgg cgtggcgcgc ccgcagattt ttgctttcga
    37741 tactgagaac gcgttgatta acgttaccgg cacggcaagt tttgcttcgg aacagctgga
    37801 tttgactatt gatccggaga gtaaaggtat tcggattatc acactgcgtt cgccgctgta
    37861 tgtgcggggg acgtttaaaa atccgcaggc tggagtgaaa gccggaccgt tgattgcccg
    37921 tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg gcggcggcgt tactggcgct
    37981 gatctcacct tccgaagggg aggctaatca gtgtcggacg attttgtcgc agatgaagaa
    38041 gtgatgataa ttgccggatg tctgacatcc ggcaattaaa cagatttaga ttgttggcaa
    38101 agtgtgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaatccttgt
    38161 gaattcaata tattgtatgg aaattcaggc ctgataagcg tagcgcatca ggcttttcac
    38221 acttattaca acgactgatg tcgtgtctca tgggtcagca gcagggcgat taacgtcaag
    38281 ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagt ctgcaaccag
    38341 gctgcgatat atggtgcaac ggaagccccg agaatcgacg ctacgttgta agagaatgat
    38401 gctccggtgt aacgcacttc tgttgggaac agctctggta acagcgcgcc cattggcccg
    38461 aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag aatcgggttg
    38521 ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag cgtggtgata
    38581 attaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac tggcaccatc
    38641 acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg cggcaggcca
    38701 agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat gatgtaaaac
    38761 agcgtatagg ttgccagcat aatgaacgta cccagtacgg ttacgcgaac atgtttggtt
    38821 agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt ctcaaacacc
    38881 ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac cgccgagaag
    38941 ataaatggca cacgccagcc ccagctcata aactgctcat cggtcagtag ccaggaaagc
    39001 agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcccagctg tgggaaggag
    39061 ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc cgcgccgccc
    39121 cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag cagcggggcg
    39181 aaaataccaa tcgtggcata gcccggcagc aaaccaatca ccacggtcga aatccccatc
    39241 gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa atgaccaaaa
    39301 acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag cgactgtagc
    39361 gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat aacggccgca
    39421 gtggcgtaaa tataaaagtc gaagaactca atggccgtgc caatgagaga ggcgacaagg
    39481 actttattac gcgagttgat cggcgtgtat tcttgctcgt ggtcgagagt tgtggctgtt
    39541 gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc acagcaaaag
    39601 tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa ataaggcaaa aaggggataa
    39661 tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata aatggtggat
    39721 aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg ggttatgagt
    39781 tggcatgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga agagtttcaa
    39841 gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg gcctacgggg
    39901 agccaggccg aataaattcg tacgccgcat ccggcctatt ttaaggctgc gttttccccg
    39961 gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca
    40021 aacgggcgaa gaagtagaaa aatgccatca gccccagcac ggaaccaaat gctgcgccag
    40081 agggggattt catcagcgat ggcagggtgt aggtcatcac gattttaatc acttcaaaac
    40141 caatagcagc cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg tggcgcggta
    40201 accgccagaa gatccagaag aaaagcagat agttggcgaa gatggaaatc gccaggccaa
    40261 tcaaccgcca cgtcggtttc agccactcaa tgctgttcag gtgcagggcg ctaataatca
    40321 tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca
    40381 aaccaatcag cgaaataaaa tcacgcagat attttaccca gaacttctcc tgatcttgcg
    40441 gcgagcgttc ccagacatcg cgcgactggg cgcgaatcgc ttcacgcagg ttacccatcc
    40501 agttgatgcc ggaataaagc gccaccgcca ggccgacaag ccctacagtc gtacgctgct
    40561 gaacggcggt gttgatggtg tttttcaacg tggcggctag cgtcggatcg ctgatgtttt
    40621 gcagaatttt gtcgaagata tcctgtagca acatcggatg ggaggccagc acaaagcccc
    40681 ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa aaatgagaaa taggtgatag
    40741 ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg
    40801 caataaccgg ttggcgttgg actttttcgg cagtggtggt gacggtttcc agcgcctggc
    40861 taactttgcc gcctttctcg ctattatcca acggcttaat cggcttgtgc tccagatcct
    40921 gggtgggacg tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt
    40981 cattcttaaa aagtatagtc ggtcagtcca ggtactcttt cattacgcca gccagccact
    41041 gcataaacag gtttacacgc cgggaaagct cccgacgctg cggataaacc agcgaaaggg
    41101 agagcggctc ggcacggtag ccaggtaata cttcaataag ccgcccggca cgcagggctt
    41161 cgcgcacggc aatgcgcggg atctgaataa tccccagacc ggcaagaccg gcggcgagat
    41221 agttttcgct actgtttacc gtcaacatgc cgccagactt aaaccactgg acgccattaa
    41281 cgctggcaac ctcaaaacct aacggatgta cacccaggtg cggtgtataa cgcactattg
    41341 cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac
    41401 tggcacagtt gaccatggtc agtttgccga ggggacgggc gataacgcca tcttacggta
    41461 acgcgccagt gcgtatcacg caatcaaaac catcatgaag aatatctacc ggacggtcat
    41521 ggctactcag ttccagctca attcccggat gctgatagag aaattccgac aggcgcggca
    41581 gtaacaggct tttcgcgatt cctggcggga tgtcgatgcg taatttaccg ctgatactgg
    41641 tggcatcctg ctgaaacaga ccgtccagtt cgttgagatt actcaacaca tcttttgctc
    41701 gttgataata ggtcatgcct tctggcgtga gtttgacccg tcgcgtggtg cgctggagaa
    41761 gctgggtgcc aagttgatgt tccagtgcct gtatctggcg cgaaacactt ccttttggca
    41821 aagcaaataa atccgccgcg cgggaaaaac tttccagctc cgcgactttg atgaacaact
    41881 gcattgcgtg aattttatcc atttctttga ccgattgttg ttcacagcga aacagtaacg
    41941 cgcatatcgc attgtttatt gttttttcta acatctaatg ccgggtttca caatgttgtc
    42001 atgatgaatg tggcgatttc tcagcaggaa aagggcggaa tagctttctg agaaaggtga
    42061 atccccttaa acagagcgta tttttcagcg attcccttta aagtgaatag aggacgtatt
    42121 ggctatttgt gcttattatt gtctcattgc cgtttgttat tttggcttgt ggtcgcggaa
    42181 tttcattttt aattgcatgc gtactaacca tcaatataag gaaatcctca aaattgtttt
    42241 aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt aactatttat atcggtaact
    42301 attttctacg cgtcatctct gtagaatggc caccgccaaa tggcgaattc ttattttttc
    42361 tcgcgggtaa tacccttcca ctacttaagt ctttttaaca tattcaggtg gagattcagt
    42421 tatgcaaata gtcatgtttg acaggcagtc aatatttatt catggaatga aaatcagttt
    42481 acagcagcgt attccaggag tgagtattca gggggccagt caggcagacg agttatggca
    42541 aaagctggaa agttaccctg aagccttagt tatgctcgat ggcgatcagg atggtgagtt
    42601 ttgctactgg ttgctgcaaa aaaccgtggt gcaatttcct gaggttaagg tgttaattac
    42661 ggcgacggat tgcaataaac ggtggttaca ggaagttatc cattttaatg tgctggccat
    42721 tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg
    42781 gatgatgttt ctgcctggag actggcggac gacgccggaa aaggacatta aagatctcaa
    42841 atcgttaagc gcccgccagc gggagatttt aaccatgtta gcggcgggcg aatcaaataa
    42901 ggagatcggc agagcgctga atatcagtac cggaacggta aaagcgcatc tggaatccct
    42961 gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca atgatgttaa atatctcctc
    43021 ctgaagagga taattttaca gccagcagga agttagcggc tggctgtaaa aatattatgg
    43081 ttcgccgtac aaaccaatta aacggcgtac cacaccgtta gtccagccaa acccatcctg
    43141 caacggatac tcgccaccgc cgccttcgcg gggaacacca tcggcaatat ggtatttttc
    43201 gatcagtttg tgctgttcca gatagaactg attcaccgtc ttcagccagc ttcgcgcgat
    43261 ttcatcaccc agaaggtcat cgccatacat tttaaatccc tgaattgcca tccactgtaa
    43321 cggtgcccag ccgttgggtt tatcccactg ttcaccggtt tcgtactcgc ttgccagaat
    43381 cccgccaggt gtcagtaacc gactgcgcac ggcgtttgcc agacgatcgg cctgttcatg
    43441 gttcgccata ccgacataga gtggcacaat ggcggcagcg gaaaacagcg ccagttgttc
    43501 gcgtcgccag tcgtaatcgc ggtagatgcc gttttcatca tcccagaggt aacggtttac
    43561 cgcatcgcga cgggcactgg ccttctggcg gaacagtgct tctgtctctt tctcaccttt
    43621 cagcgccgag atgttggcga tggcgctctc cagtttaaac aggaaggcat tcagatcgat
    43681 ggggatgaac tgggtggtac gaatgctcgc cagacgacca gtatcacgca gccaacggga
    43741 agagtaatcc caaccggagg ccgcccccgc gcgtaaatcg cggtacacct cgttgggcgg
    43801 gcgaccagaa tgtttcgcgg tttcaacgtc ctcaagccag gattcgtcac gcggcgtgtc
    43861 gcgatcgtcc cagtaacggt tgagcagcga tccgtccggc atccgcacaa catggcgata
    43921 ggcctgatta gggatcaacg attctgcacc gtccatccag aaggcatatt ccattttcag
    43981 gtggtcgaga tagcggcgcg caccgcgtac accatcttct tcaaacaact ccaccatcag
    44041 cgcaaaaacc ggtggttgcg agcggctcaa ataataggtg cggttgccgt tggggatgtg
    44101 accgtagttt tcgatcatcc aggcgaagtt atcggccatg catttcagca aatcttcccg
    44161 accactttcc gccagcccca gcatggtgaa gtaggaatcc caatagtagg tttcgctaaa
    44221 acggccgccc gggacaatat atgactgcgg cagcgccagc agagaagacc acggaatgtg
    44281 atcctgtggt tcgcgggtta gcaccggcca caactggtcg atatgctctt tcagggaatt
    44341 ttgcgggtcc gatacatact cgctggagta gacctccggc agccagaagt gattttcaac
    44401 aaacttgcgc aagtcaaaat cacgatgacg gcgcacttta cggtagcgga ttaaaatatc
    44461 cagcgggtcc attttgggtg cgcagtcggg aaaggtttta ctgtcgggga aaattttcgc
    44521 cgactgaaca tgctcgaaca gttcgagata gcgatcggca ggcgtcagcg catcagaggc
    44581 gggcagccct tcaatcatct cgggttccgg ttctgcctcg atcatctcat ccagttttaa
    44641 ttcatacggg tccagttcat agcagagatc gacctcgatc atcagttcgt ctggattagg
    44701 gttttgaatt ttctgattga gcataaggat ccaacctccg aaattcgtcg ctaattatcg
    44761 ggcttttctt taagggtagt cattcgctcc gcctggtggg cgacaaagcg tgcggtggat
    44821 cacgtttatt aacctgtcaa aaaatataac gcactaatat ccatggaatt tataaaaata
    44881 cagatttcgt gttgtttttc attttcagaa cattca
//
LOCUS       DAZUGK010000015        44298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZUGK010000015 DAZUGK010000000
VERSION     DAZUGK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.015"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1488
                     /gene="xanQ"
                     /locus_tag="ACHY5W_000716"
     CDS             88..1488
                     /gene="xanQ"
                     /locus_tag="ACHY5W_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFS4445667.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            1506..2822
                     /gene="guaD"
                     /locus_tag="ACHY5W_000717"
     CDS             1506..2822
                     /gene="guaD"
                     /locus_tag="ACHY5W_000717"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFS4445668.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            2858..4225
                     /gene="ghxQ"
                     /locus_tag="ACHY5W_000718"
                     /gene_synonym="ygfQ"
     CDS             2858..4225
                     /gene="ghxQ"
                     /locus_tag="ACHY5W_000718"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFS4445669.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4261..4749)
                     /gene="ygfS"
                     /locus_tag="ACHY5W_000719"
     CDS             complement(4261..4749)
                     /gene="ygfS"
                     /locus_tag="ACHY5W_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS4445670.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALAMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRIRQQRIVASGEN
                     PL"
     gene            complement(4749..6668)
                     /gene="ygfT"
                     /locus_tag="ACHY5W_000720"
     CDS             complement(4749..6668)
                     /gene="ygfT"
                     /locus_tag="ACHY5W_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFS4445671.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSYSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGNIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            7104..8552
                     /gene="uacT"
                     /locus_tag="ACHY5W_000721"
     CDS             7104..8552
                     /gene="uacT"
                     /locus_tag="ACHY5W_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFS4445672.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSELFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            8554..8679
                     /gene="yqfG"
                     /locus_tag="ACHY5W_000722"
     CDS             8554..8679
                     /gene="yqfG"
                     /locus_tag="ACHY5W_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFS4445673.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(8676..8747)
                     /locus_tag="ACHY5W_000723"
     CDS             complement(8676..8747)
                     /locus_tag="ACHY5W_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445674.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            8802..9350
                     /gene="idi"
                     /locus_tag="ACHY5W_000724"
     CDS             8802..9350
                     /gene="idi"
                     /locus_tag="ACHY5W_000724"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4445675.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(9393..10910)
                     /gene="lysS"
                     /locus_tag="ACHY5W_000725"
     CDS             complement(9393..10910)
                     /gene="lysS"
                     /locus_tag="ACHY5W_000725"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4445676.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(10920..12018)
                     /gene="prfB"
                     /locus_tag="ACHY5W_000726"
     CDS             complement(join(10920..11942,11944..12018))
                     /gene="prfB"
                     /locus_tag="ACHY5W_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4445677.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(12109..13842)
                     /gene="recJ"
                     /locus_tag="ACHY5W_000727"
     CDS             complement(12109..13842)
                     /gene="recJ"
                     /locus_tag="ACHY5W_000727"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4445678.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(13848..14558)
                     /gene="dsbC"
                     /locus_tag="ACHY5W_000728"
     CDS             complement(13848..14558)
                     /gene="dsbC"
                     /locus_tag="ACHY5W_000728"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFS4445679.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(14583..15479)
                     /gene="xerD"
                     /locus_tag="ACHY5W_000729"
     CDS             complement(14583..15479)
                     /gene="xerD"
                     /locus_tag="ACHY5W_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4445680.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            15591..16112
                     /gene="fldB"
                     /locus_tag="ACHY5W_000730"
     CDS             15591..16112
                     /gene="fldB"
                     /locus_tag="ACHY5W_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFS4445681.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(16152..16559)
                     /gene="ygfX"
                     /locus_tag="ACHY5W_000731"
     CDS             complement(16152..16559)
                     /gene="ygfX"
                     /locus_tag="ACHY5W_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFS4445682.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(16540..16806)
                     /gene="sdhE"
                     /locus_tag="ACHY5W_000732"
     CDS             complement(16540..16806)
                     /gene="sdhE"
                     /locus_tag="ACHY5W_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFS4445683.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            17049..18029
                     /gene="ygfZ"
                     /locus_tag="ACHY5W_000733"
     CDS             17049..18029
                     /gene="ygfZ"
                     /locus_tag="ACHY5W_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFS4445684.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTFAPDDERVLLGVAGFQARAALAKLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(18106..18765)
                     /gene="yqfA"
                     /locus_tag="ACHY5W_000734"
     CDS             complement(18106..18765)
                     /gene="yqfA"
                     /locus_tag="ACHY5W_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4445685.1"
                     /translation="MVQKPLVKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(18929..19240)
                     /gene="yqfB"
                     /locus_tag="ACHY5W_000735"
     CDS             complement(18929..19240)
                     /gene="yqfB"
                     /locus_tag="ACHY5W_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFS4445686.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            19285..20718
                     /gene="bglA"
                     /locus_tag="ACHY5W_000736"
     CDS             19285..20718
                     /gene="bglA"
                     /locus_tag="ACHY5W_000736"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFS4445687.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(20882..23755)
                     /gene="gcvP"
                     /locus_tag="ACHY5W_000737"
     CDS             complement(20882..23755)
                     /gene="gcvP"
                     /locus_tag="ACHY5W_000737"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFS4445688.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQVGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(23873..24262)
                     /gene="gcvH"
                     /locus_tag="ACHY5W_000738"
     CDS             complement(23873..24262)
                     /gene="gcvH"
                     /locus_tag="ACHY5W_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFS4445689.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(24286..25380)
                     /gene="gcvT"
                     /locus_tag="ACHY5W_000739"
     CDS             complement(24286..25380)
                     /gene="gcvT"
                     /locus_tag="ACHY5W_000739"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFS4445690.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQVKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            25671..25754
                     /locus_tag="ACHY5W_000740"
     CDS             25671..25754
                     /locus_tag="ACHY5W_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445691.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(25828..27030)
                     /gene="ubiI"
                     /locus_tag="ACHY5W_000741"
                     /gene_synonym="visC"
     CDS             complement(25828..27030)
                     /gene="ubiI"
                     /locus_tag="ACHY5W_000741"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFS4445692.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(27053..28231)
                     /gene="ubiH"
                     /locus_tag="ACHY5W_000742"
                     /gene_synonym="visB"
     CDS             complement(27053..28231)
                     /gene="ubiH"
                     /locus_tag="ACHY5W_000742"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFS4445693.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(28228..29553)
                     /gene="pepP"
                     /locus_tag="ACHY5W_000743"
     CDS             complement(28228..29553)
                     /gene="pepP"
                     /locus_tag="ACHY5W_000743"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFS4445694.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(29579..30157)
                     /gene="ygfB"
                     /locus_tag="ACHY5W_000744"
     CDS             complement(29579..30157)
                     /gene="ygfB"
                     /locus_tag="ACHY5W_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFS4445695.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            30325..30654
                     /gene="zapA"
                     /locus_tag="ACHY5W_000745"
     CDS             30325..30654
                     /gene="zapA"
                     /locus_tag="ACHY5W_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4445696.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            30696..30879
                     /gene="ssrS"
                     /locus_tag="ACHY5W_000746"
     ncRNA           30696..30879
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHY5W_000746"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            30900..31502
                     /gene="fau"
                     /locus_tag="ACHY5W_000747"
     CDS             30900..31502
                     /gene="fau"
                     /locus_tag="ACHY5W_000747"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4445697.1"
                     /translation="MTHLPELPLILSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKIWEW"
     gene            complement(31603..31662)
                     /gene="ibsC"
                     /locus_tag="ACHY5W_000748"
     CDS             complement(31603..31662)
                     /gene="ibsC"
                     /locus_tag="ACHY5W_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFS4445698.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(31995..32054)
                     /gene="ibsC"
                     /locus_tag="ACHY5W_000749"
     CDS             complement(31995..32054)
                     /gene="ibsC"
                     /locus_tag="ACHY5W_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFS4445699.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(32283..33515)
                     /gene="serA"
                     /locus_tag="ACHY5W_000750"
     CDS             complement(32283..33515)
                     /gene="serA"
                     /locus_tag="ACHY5W_000750"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4445700.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(33771..34430)
                     /gene="rpiA"
                     /locus_tag="ACHY5W_000751"
     CDS             complement(33771..34430)
                     /gene="rpiA"
                     /locus_tag="ACHY5W_000751"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4445701.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(34486..34716)
                     /locus_tag="ACHY5W_000752"
     CDS             complement(34486..34716)
                     /locus_tag="ACHY5W_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4445702.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTMRLANGV"
     gene            34858..35751
                     /gene="argP"
                     /locus_tag="ACHY5W_000753"
     CDS             34858..35751
                     /gene="argP"
                     /locus_tag="ACHY5W_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFS4445703.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            35954..38036
                     /gene="scpA"
                     /locus_tag="ACHY5W_000754"
                     /pseudo
     CDS             35954..38036
                     /gene="scpA"
                     /locus_tag="ACHY5W_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            38029..39024
                     /gene="meaB"
                     /locus_tag="ACHY5W_000755"
                     /pseudo
     CDS             38029..39024
                     /gene="meaB"
                     /locus_tag="ACHY5W_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
     gene            39035..39820
                     /gene="scpB"
                     /locus_tag="ACHY5W_000756"
     CDS             39035..39820
                     /gene="scpB"
                     /locus_tag="ACHY5W_000756"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFS4445704.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            39844..41322
                     /gene="scpC"
                     /locus_tag="ACHY5W_000757"
     CDS             39844..41322
                     /gene="scpC"
                     /locus_tag="ACHY5W_000757"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFS4445705.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(41319..42215)
                     /gene="ygfI"
                     /locus_tag="ACHY5W_000758"
                     /pseudo
     CDS             complement(41319..42215)
                     /gene="ygfI"
                     /locus_tag="ACHY5W_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(42382..43122)
                     /gene="yggE"
                     /locus_tag="ACHY5W_000759"
     CDS             complement(42382..43122)
                     /gene="yggE"
                     /locus_tag="ACHY5W_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFS4445706.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(43215..43850)
                     /gene="argO"
                     /locus_tag="ACHY5W_000760"
     CDS             complement(43215..43850)
                     /gene="argO"
                     /locus_tag="ACHY5W_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFS4445707.1"
                     /translation="MISYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLVLVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            44057..>44298
                     /locus_tag="ACHY5W_000761"
     CDS             44057..>44298
                     /locus_tag="ACHY5W_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445708.1"
                     /translation="MHAYDLTLLPHPIRENMPRQQGWCFGLPPGITPEQWPLDPNNGF
                     PLNHGFTLLLPEDYRIYGPEIVALSFFAVAPEHRSQ"
BASE COUNT        10609 a        11790 c        11125 g        10774 t
ORIGIN      
        1 cgttctcaag gcgtcctgcg ccccaaattc gtgggaattt attttactaa ttcagatgat
       61 caaatttact ttaaaaggag tgaagggatg tctgatataa accatgcagg ttctgacctt
      121 atatttgaac tggaggatcg ccctcccttt catcaggctc tcgttggtgc cattacccat
      181 ctgttggcaa ttttcgttcc gatggtaacc cccgcgttaa tcgtgggtgc ggccttacag
      241 ctttccgctg aaacaaccgc ctatcttgtt tctatggcga tgatcgcctc tggtattggt
      301 acctggttac aagtaaaccg ctacggcatc gtcggttctg gcctactctc aattcagtca
      361 gtcaattttt catttgttac ggtcatgatt gcgctgggca gcagcatgaa aagcgacggt
      421 tttcacgaag agttaatcat gtcgtcgctt ctcggcgtct ccttcgttgg cgcatttctg
      481 gttgtcggat cttcatttat cttgccctat ttacgtcggg ttattacgcc taccgtcagc
      541 ggtattgtgg tactgatgat cggcttaagc ctgattaaag tcggcattat cgattttggt
      601 ggaggatttg cagccaaaag cagcggtacg ttcggcaatt acgaacatct cggcgttggt
      661 ttattggttt taattgtggt gatcggcttt aactgctgtc gcagtccgtt gctacgcatg
      721 ggagggatcg ccattgggct atgtgtcggc tatatcgcat cgttatgcct gggcatggtg
      781 gatttcagca gtatgcgcaa tttgccgtta atcaccatcc cgcatccgtt caaatacggc
      841 tttagtttta gcttccatca gttcctggtg gttggcacga tttatctgct tagcgtgctg
      901 gaagcagtcg gcgatatcac cgccacggca atggtttccc gccgccccat tcagggggaa
      961 gagtatcagt cccgactgaa aggcggcgtg ctggcagatg gtctggtttc tgttatcgcc
     1021 tccgctgttg gttcattacc attaaccacg tttgcgcaaa ataatggggt tattcagatg
     1081 actggcgtcg cttcacgtta tgtcgggcga accatcgcgg taatgctggt tatcctcggc
     1141 ttatttccga tgattggcgg cttcttcacg accattccct cggcagttct gggaggcgca
     1201 atgacgttga tgttttccat gattgccatc gctgggattc gcatcatcat caccaacgga
     1261 ttaaagcggc gtgaaacact tattgtcgcc acttctttag gtttagggct tggcgtctcc
     1321 tacgatcccg aaatttttaa aatattgcca gcctctattt atgtattagt tgaaaaccct
     1381 atttgtgctg gcgggttaac tgcgatttta ttaaatatta tcctccctgg tggctaccga
     1441 caggaaaacg ttctgcctgg tattacctca gcggaagaga tggattaaca gtaaaggagt
     1501 caatgatgtc aggagaacac acgttaaaag cggtacgagg cagttttatt gatgtcaccc
     1561 gtacggtcga taacccggaa gagattgcct ctgcgctgcg gtttattgag gatggtttat
     1621 tactcattaa acagggaaaa gtggaatggt ttggcgaatg ggaaaacgga aaacatcaaa
     1681 ttcctgacac cattcgcgtg cgcgactatc gcggcaaact gatagtaccg ggctttgtcg
     1741 atacacatat ccattatccg caaagtgaaa tggtgggggc ctatggtgag caattgctgg
     1801 agtggttgaa taaacacacc ttccctactg aacgtcgtta tgaggattta gagtacgccc
     1861 gcgaaatgtc ggcgttcttc atcaagcagc ttttacgtaa cggaaccacc acggcgctgg
     1921 tgtttggcac tgttcatccg caatccgttg atgcgctgtt tgaagccgcc agtcatatca
     1981 atatgcgtat gattgccggt aaggtgatga tggaccgtaa cgcaccggat tatctgctcg
     2041 acactgccga aagcagctat caccaaagca aagaactgat tgaacgctgg cacaaaaatg
     2101 gtcgtctgct atatgcgatt acaccacgct tcgcccctac ttcttctcct gaacagatgg
     2161 cgatggcgca acgcctgaaa gaggaatatc cggatacgtg ggtacatacc catctctgtg
     2221 aaaacaaaga tgaaattgcc tgggtgaaat cgctttatcc tgaccatgat ggttatctgg
     2281 atgtttacca tcagtacggc ctgaccggta aaaactgtgt ctttgctcac tgcgtccatc
     2341 tcgaagaaaa agagtgggat cgtctcagcg aaaccaaatc cagcattgct ttctgtccga
     2401 cctccaacct ttacctcggc agcggcttat tcaacttgaa aaaagcatgg cagaagaaag
     2461 tcaaagtggg catgggaacg gatatcggtg ccggaaccac tttcaacatg ctgcaaacgc
     2521 tgaacgaagc ctacaaagtg ttgcaattac aaggctatcg cctctcggca tatgaagcgt
     2581 tttacctggc cacgctcggc ggagcgaaat ctctgggcct tgacgatttg attggcaact
     2641 ttttacctgg caaagaggct gatttcgtgg tgatggaacc caccgccact ccgctacagc
     2701 agctgcgcta tgacaactct gtttctttag tcgacaaatt gttcgtgatg atgacgttgg
     2761 gcgatgaccg ttcgatctac cgcacctacg ttgatggtcg tctggtgtac gaacgcaact
     2821 aataataaaa ctttaacatc ctcgtgagga catcattatg tctggagaca tcctacaaac
     2881 accggacgca ccaaagccac agggcgcgct ggataattat tttaaaatta ccgctcgtgg
     2941 cagtaccgtt cgtcaggaag tactggctgg cttaacgacc tttctggcta tggtttattc
     3001 cgttatcgtc gttccgggaa tgctgggcaa agcaggtttt cctcccgcag ctgtgtttgt
     3061 tgccacctgt ctggtcgcgg gcttcggctc gttgctgatg ggattatggg ccaatttgcc
     3121 aatggcgatt ggttgcgcga tttccttgac ggcgtttacc gcattcagtc tggtactcgg
     3181 gcaacaaatt agcgttcctg tcgcactggg cgcggtattt ctgatgggcg tcatcttcac
     3241 cgccatttcc gtaaccggtg tgcgtacctg gatcttacgt aatttgccga tgggtatcgc
     3301 tcacggtaca ggtatcggta tcgggctgtt tctgctgctg attgctgcta acggtgtggg
     3361 tatggttatc aaaaacccga ttgaaggctt gccagtggcg ctcggtgcgt ttacctcctt
     3421 cccggtgatg atgagcttgc tggggctggc ggtcatcttc ggtctggaga agtgtcgcgt
     3481 acccggcggg atcttgttgg tgattattgc aatttcgatc atcggcttaa tctttgaccc
     3541 agcggtgaaa taccacggtc tggtggcgat gccaagcctg actggcgaag atggtaagtc
     3601 tctgattttc agcctcgata ttatgggcgc actccagcca actgtacttc cgagtgtact
     3661 ggcattggtg atgaccgcag tgttcgacgc tactggcacc atccgtgccg tcgccggtca
     3721 ggcgaatttg ttggataaag acaaccagat catcaacggc ggcaaagccc tgaccagtga
     3781 ctcagtaagt tcaatattct ccggcctggt gggcgcagcg cccgcagcgg tttatatcga
     3841 atcagcggca ggaaccgccg ccgggggtaa aacagggttg accgcaaccg tagtgggggc
     3901 gttattcctg ctgattctgt tcttatcacc gctgtcattt ttgatccctg gttacgccac
     3961 tgcacccgct ctgatgtacg taggtttgct gatgttaagt aacgtctcga agctggattt
     4021 caatgatttt attgacgcta tggctggcct ggtgtgtgcc gtgttcatcg ttctgacttg
     4081 taatatcgtt accggtatta tgctgggctt tgtgacactg gtcgtaggcc gcgtctttgc
     4141 acgcgaatgg caaaagctga atattggtac ggtgatcatt actgccgcac tggtcgcatt
     4201 ttacgcaggt ggttgggcaa tctaatagat tctccgcgcc tttcagcgcg gagatttctt
     4261 tcagagagga ttctcaccgc tggcaacaat acgctgctgg cggattcgcc tgagttctct
     4321 ttcagtaagt aactgcagcg cctgtgtcgg gcaagattca acgcaggctg gcccctcttc
     4381 ccgctgttca cagagatcgc atttcacgat ttgttgccgg gtatcccccg gcaacgactg
     4441 aatggtgatc atcccaaacg gacacgcact gacgcagctc tgacagccaa tacaacgggc
     4501 agaattggtc tgcaccacct gctcgcccat cgccagcgcc cccacggggc aagcgccaac
     4561 acaaggggcg ttttcacatt ggtggcacat caccggcgca ctgatgcagt ccagtcgctg
     4621 taccttcagc cggggtaaaa agatatcggc attcaactcc tgttctgaag ggtgagcgac
     4681 cacacaggcc acttcacaag tacggcagcc aatacagtca gccggattaa cgataattaa
     4741 cgatttcatc acgatgcctt cgtatcaaac agagttaaca tatcgcgcgc cgcctgcctt
     4801 cctgcggcca tggcagtgac aaccagatcc gcgccatgaa ctgcatcccc accagcaaag
     4861 acttttttca gatgcgtctg ggtaggtaaa tacccaacgt cgccggtttg aatcaggccc
     4921 catttatcga gtttaattcc gctgccctgc aaccacggca tggcatgcgc ctggaaacca
     4981 aaggccataa tgagaacatc ggcgggcaat tcaaactctg aacccgcaac cggacgagga
     5041 cgacggcgac catccggccc cggctcaccc atggcggtac gaatcaggcc caccgcagtt
     5101 aaacgcccat cttcgtcaca agcgatatat tgcggctgaa cattgaactg aaactcgaca
     5161 ccttcctcgc gcgcattgac cacctctttg cgcgagcccg gcatactgac ttcatcacga
     5221 cgatacgcgc aggtcacgct ggcagcattg aggcgaatgg aagtccgcaa acaatccatt
     5281 gtcgtatcgc caccgcccaa taccacgact cgcttacctt ccacgtccgt cagcggatac
     5341 tcttcagact ccggcaatcc catgagctgg cgggtatggg cagtcaggaa cggtagagcc
     5401 tgaatgacac cgggcgcatc ttcatgcggc agatctgctc gcatcatccc gtaagtcccc
     5461 acgccaatga aaactgcatc atattcagaa gttaaatcgc taaaggtaat atcgcggcca
     5521 atttcacagt taagatgaaa gtcgattccc attgcggtga atatctctcg ccgctggctt
     5581 aataccgttt tatcgagttt gaaaggagga atgccaaaag tcagcatacc gccaatttct
     5641 ggatggcgat caaagacatc gacctgaact cctgcgcgcg ccagaatatc agcacaccct
     5701 aaccctgcag gcccagcgcc aatcaccgcc actttctcga tacggggaac aactttgctg
     5761 acatcgggac gccagcccat cgccagcgcg gtatcggtga tgtagcgttc cagattaccg
     5821 atagagactg cgccagagtg atctttcaaa gtacatgcgc cttcacaaag acggtcctgt
     5881 ggacataccc tgccgcagat ttctggtaag gaactggtct ggtggcaaag ttctgccgct
     5941 tcaataatat ttccttcctg taccagacgg atgtaatccg gaatagcgtt atgcagcggg
     6001 caatgccagt tgcagttagc tttttcggca caataaacac agcggtcact ctcataagtc
     6061 gcttgcagtg gatccagccc gcaatagatt tcgccaaagt gggttttccg ttcactcgct
     6121 gaaattttat ctgcgccttt acgcgagtta acggggagca acgctgcact gcgagatggc
     6181 tgagcgtctg atgacgcttt tcctgctgcc gttttgcgct ggcgggccac ctttatctgc
     6241 tgtaaccctt tatcgtccat cagtcgtaac gcctgtgttg ggcaaacttc aatacaggct
     6301 tgcgtgccgg aactgcgctg gttacaaagg tcgcatttct gcgcaatcgt atcgaccatc
     6361 tcaacgacgc caaaggggca agcgattgcg catcttttac aaccaataca tttttgctcg
     6421 tccagttgta cgctatcgga ctggaaagtc agagcattaa ccggacaagc cgtaacgcaa
     6481 ggggcattgt tgcagtgatg gcaggccacc ggattcgcag ccaggccttt ccctacaacg
     6541 tggatacgtg gtcgaaagtc actgtaactc agcggccagt tttcttgatt gtgtgccacc
     6601 gcacaggcaa tttcacaagc atgacagcct atacattccg cagcttcagc agcgataaac
     6661 ttattcattt gcatcccttt catttaatga gttatgtctt ctcaaatttc gcgatgcaat
     6721 aaacgggcat atattaggta gcatgaccgt tttaattata attttgcgac tgagttcaaa
     6781 ttattgcccc tcaatctatg ccgctttcat tatttcaaca ttgccgcccc aaatagcgct
     6841 atccagataa aaacgattaa atgcgagagt gcgacatgcc agaatgatta atgaaatatc
     6901 aatatgagaa aattacaggt aattccattg tcaggttctc ttctcatttt cgtggtttaa
     6961 gcacgattat gtgattgcct ccgcaattcc tgtctctaac tccccttcct cgcaaaaact
     7021 ggcactccac gagcatgtgt ttagacagtt tcattaacgt aaacggttgc tttttactct
     7081 ggcgggcgaa aggagaaaca ctgatgagcg ccatagattc ccaacttccc tcatcttctg
     7141 ggcaagaccg cccaactgat gaggttgacc gcatattatc accaggaaag ctgatcatac
     7201 tcggtctgca acacgtcctt gtcatgtacg caggtgcagt cgctgttcct cttatgattg
     7261 gtgaccgact gggcctctca aaagaagcta ttgcgatgct cattagctcg gagctctttt
     7321 gctgcgggat cgtcacatta ttgcaatgta tcggtatcgg ccgctttatg gggatccgcc
     7381 tgccggtgat tatgtcggta acgtttgctg ctgtaacacc aatgatagcc attgggatga
     7441 acccggatat cggcctgctg gggatatttg gtgccactat cgccgcgggt tttatcacca
     7501 cattattagc gccacttatc ggtcgcttga tgcctttatt tccgccactg gttaccggtg
     7561 tggttattac ttctatcggg cttagcatca ttcaggtggg tattgactgg gccgccggag
     7621 gtaaagggaa tccgcaatat ggtaatcccg tttatttagg tatctccttt gccgtcttaa
     7681 tttttatctt gctcattact cgctatgcga aaggatttat gtccaacgtc gccgtattac
     7741 tggggattgt atttggcttt ttactttcgt ggatgatgaa tgaagtcaat ttatccgggc
     7801 tacatgatgc ctcgtggttt gcgattgtca ccccgatgtc gtttggtatg ccgattttcg
     7861 atcccgtttc cattctgacc atgactgccg tgttaatcat cgtgtttatc gagtcgatgg
     7921 ggatgttcct ggcactgggt gaaatagtcg gtcgtaaact ctcttcgcac gatattattc
     7981 gcgggctgcg tgtcgatggc gtagggacaa tgataggcgg aacgtttaac agcttccccc
     8041 atacgtcatt ttctcaaaac gttggcctgg ttagcgtgac gcgcgttcat agccgctggg
     8101 tgtgtatttc ttcgggaatt atattaatcc tgtttggcat ggtgccaaaa atggcggtgc
     8161 tggtagcctc cattccgcaa tttgtgctgg gcggcgctgg tctggtgatg ttcggcatgg
     8221 tactggcgac agggattcga attctgtcgc gctgtaacta caccaccaac cgttacaacc
     8281 tctatattgt ggcgatcagt ctcggcgttg gcatgactcc gacgctctct cacgatttct
     8341 tttctaagtt accggccgta ctgcaaccgc tgctacatag cggcattatg ctcgcaaccc
     8401 ttagcgccgt tgtgctgaat gtcttcttta atggctacca gcatcatgct gacctggtga
     8461 aggaatccgt ctctgataaa gatttaaaag tcaggacagt acgtatgtgg cttctgatgc
     8521 gcaagctgaa gaaaaatgag catggagaat aatatgaatt ttttaatgcg cgctatattc
     8581 agtctgctgt tgctttttac tctctctatt cctgtcattt ctgactgtgt tgcaatggcc
     8641 attgaaagtc gcttcaaata tatgatgcta cttttttaaa tggtttttac ctgtcgacat
     8701 ccgctcaaaa cgggcggttg tcgataaacg ctcacttggt taatcatttt actcttcaat
     8761 tatctataat gatgagtgat cagaattaca tgtgagaaat tatgcaaacg gaacacgtca
     8821 ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc gcacacacgg
     8881 cagacacccg cttacatctc gcgttctcca gttggctgtt taatgccaaa ggacaattat
     8941 tagttacccg ccgcgcactg agcaaaaaag catggcctgg cgtgtggact aactcggttt
     9001 gtgggcaccc acaactggga gaaagcaacg aagacgcggt gatccgccgt tgccgttatg
     9061 agcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc tatcgcgcca
     9121 ccgatccgaa tggcattgtg gaaaatgaag tgtgtccggt atttgccgca cgcaccacca
     9181 gtgcgttaca gatcaacgat gatgaagtga tggattatca atggtgtgat ttagctgatg
     9241 tattacgcgg tattgatgcc acgccgtggg cgttcagtcc gtggatggtg atgcaggcga
     9301 caaatcgcga agccagaata cgattatctg catttaccca gcttaaataa aaaaccccga
     9361 catttgccgg ggttgtgagc ataacgtaat gcttatttca ccggacgcat cgccgggaac
     9421 agaataacgt cgcggatggt atggctgttg gtgaacagca ttaccatacg gtcgatgcca
     9481 atccccagac ctgctgtcgg cggtaagcca tgttccagtg cggtgacgta atcttcatcg
     9541 tagaacatcg cttcgtcgtc acctgcatct ttcgcggcaa cctgatccag gaagcgctgc
     9601 gcctgatctt ccgcgtcatt cagttcgcta aagccgttac cgatttcacg accaccaatg
     9661 aagaactcaa agcggtctgt gatttccggg ttaacgtcgt tacgacgcgc cagcggagaa
     9721 acttctgccg gatattcagt aatgaaggtc ggctgaatca gatgtgcttc agccacttct
     9781 tcgaagatct cggtaacgat acggcccaga ccccagctct tctcaacgtg gataccgata
     9841 gattcagcaa tcgctttcgc agagtcgaag ttgtccagat ccgccatgtc ggtttccggg
     9901 cgatatttct tgatcgcttc acgcatggtc agtttttcga acggtttacc gaagtccagc
     9961 gtcacgtcgc cgtaggtcac ttccgtctta ccgagaatat cctgtgccag agtacggaac
    10021 agcgattcgg tcagctcgat cagatctttg taatctgcgt aagccatgta gagttccatc
    10081 atggtgaact ctgggttatg acgtacggag ataccttcgt tacggaagtt acggttgatt
    10141 tcgaatacac gctcgaagcc accgaccacc agacgcttga ggtacagttc cggcgcgata
    10201 cgcaggtaca tgtcgagatc cagcgcgtta tggtgggtga taaacggacg cgcagcggca
    10261 ccgccaggga tcacctgcat catcggcgtt tcaacttcca taaagccgcg gttcaccatg
    10321 aactggcgaa taccagagag gatctgcgag cgcactttaa aggtgttacg ggattcatcg
    10381 ttagagatga gatccagata acgctgacga tagcgcgctt cctgatcctg caagccgtgg
    10441 aatttatccg gcagcggacg cagtgctttg gtcagcagac gcagctcggt acagtggata
    10501 gacagttcgc cggttttggt tttgaacagt ttacctttcg cgccgaggat gtcgccgagg
    10561 tcccattttt tgaactgctc gttgtaaacg ccttccggca gatcgtcacg ggcaacgtac
    10621 agctgaatgc gaccgccaac gtcctgcagg gtaacgaaag acgctttacc cataatacga
    10681 cgggtcatca tgcggccagc aacggcgact tcgatgttca gcgcttccag ttcttcgttc
    10741 tctttgccgt cgaattctgc gtgcaattgg tcagaggtat gatcgcgacg gaaatcgttc
    10801 gggaaggcaa tcccctgctc gcgcaggttc gccagcttct cacgacgcgt tttcagttca
    10861 ttgttaagat cgactaccgc gtcagcgccc tgtgcgtgtt gttcagacat gttggttcct
    10921 cataaccctg ctttcaaact tgcttcgata aattgatcca ggctgccgtc cagcacggcc
    10981 tgcgtgttgc gggtttctac cccggtgcgc agatctttaa tgcgggagtc atcaaggaca
    11041 taagaacgaa tctggctgcc ccagccgatg tcggatttgt tatcttccat cgcctgtttc
    11101 tcggcatttt tcttctgcat ctccagttca taaagcttcg ctttcatctg cttcatggcc
    11161 tggtctttgt tcttgtgctg ggaacggtcg ttctggcact gggtcacgat cccggttggg
    11221 atgtgagtaa tacgcaccgc agattcggta cggttaacgt gctgaccgcc cgcgccggac
    11281 gcgcgataaa cgtcaatgcg cagatccgcc gggttgattt cgatatcaat atcatcatca
    11341 acttccggat aaacaaacgc ggagctgaac gacgtgtggc gacgaccgcc ggagtcaaac
    11401 gggcttttac gcaccaggcg gtgaacgccg gtttctgtac gcagccagcc gtaagcgtaa
    11461 tcgccggaga ttttgatagt cacggattta atacccgcca cttcaccttc cgactcttcg
    11521 atgatttcag ttttgaaacc acgcgattct gcccagcgca gatacatacg ctcaagcatg
    11581 ctcgcccagt cctgtgcttc cgtaccgcca gaccccgcct gaatatcgag gtagcagtcg
    11641 gcactgtcat attcgccaga gaacatacgg cggaactcaa gctgcgccag tttttcttcc
    11701 agggcgtcga gttcagcaac ggcttcgtta aaggtttctt cgtcgtcagc ttctacagcc
    11761 agttccagca gaccagaaac atcttccagc ccctgtttca tttggtcgag ggtgtcgaca
    11821 acggcttcga gggaggaacg ctctttaccc agcgcctgtg cgcgttcggg ttcgttccag
    11881 acatccggct gttccagctc ggcgtttact tcttccagac gctctttctt ggcgtcgtag
    11941 tcaaagatac cccctaagaa cgtcggagcg ttccgtgagg tcctgaatgc gattatttac
    12001 cggattaatt tcaaacatgg tctgatttct tttattgagc tagtcaaaat gcggtgataa
    12061 gagcgggatt gtacccaatc cacgctcttt tttatagaga agatgacgct aaattggcca
    12121 gatattgtcg atgataattt gcaggctgcg gttgccgcga aactcgttga tatcgagctt
    12181 gtaagccagt tgcacttcgc gcacgccgtt atccggccag agggcggtat cgacattaaa
    12241 agcaatacca tccaacagcg gaccgccgcc gaccggttcg accatgactt tcaaatgacg
    12301 ttcgcccacc agccgctgtt gcagcagacg gaaatgaccg tcaaacagcg gctccgggaa
    12361 catctgcccc cacgggccag catcgcgcag cagctgcgcc acttccatgg tcatttcggc
    12421 cgggcttaac ggaccgtctg acaccacttc gccttgcaat agcgaagggt ccagccactc
    12481 agtaaccagt tcgccaaacc gttgttgaaa gagttcgaat ttatcctctt ccagcgacaa
    12541 acccgccgcc atcgcatgac cgccaaactt cagcatcatg ccagggtaga gtgtgtctaa
    12601 tcgctccagc gcatcacgca tatgcagccc ctgaatggag cgaccagaac ctttcagcgt
    12661 accatcaccc gcgggcgcaa aggcgataac cggacggtga aaacgctctt taatacggga
    12721 agccagaata ccgacaacgc cctgatgcca ttcggggtga tacattgcca gcccgccggg
    12781 tagcgtgtca cggctgcgct ccagtttctc gcacagggtc aaggcttcaa cttgcattcc
    12841 ctgttcgatc tcttttcgcg tctggtttag cgcatcgagt tcatttgcca gcacgcgcgc
    12901 ttcgccgatg ttgtcgcaca gcaagagcgc cacaccgacg gacatatcgt ccagtcgtcc
    12961 ggcagcattg agacgtggcc ccagcgcaaa acctaaatcg ctggcggcga gtttttgtgc
    13021 atcacggttt gccacttcaa gcagcgcttt aatccccgga cggcactttc cggctcggat
    13081 gcgactcatc ccctgccagg tcagaatgcg attattagcg tccaacggca cgacgtccgc
    13141 cactgtcccc agcgcgacca gatccagcag ttctgccagg ttaggaattg cgatgttacg
    13201 ctcatcaaac caaccctgat cgcgcaaaaa ggtgcgcagc gccagcatca gataaaacgc
    13261 cacacccacg cctgccagtg atttcgacgg gaaattacag tcgcgcaagt tagggttaat
    13321 gatcgcttcc gctgcgggta atgtgtcgcc tggcaaatgg tgatcggtaa caataaccgg
    13381 gatgcccaac gagcgagcgt gctcaacccc cgcatgggag gaaataccgt tatccaccgt
    13441 gacaattaac tgcgcgccac gggcatgggc ctgatcgacc acttccgggc ttaagccgta
    13501 accgtcttcg aaacggtttg gtaccaggta gtcgatattg ctgcaaccaa gcgagcgcat
    13561 cgccagcacg cttagagccg tgctggtcgc gccgtcggcg tcgaaatcac cgaccacaat
    13621 aatccgcgtt ccttcgcgaa aagcgttgta aaggatctca acggcctttt cgacgccgct
    13681 cagttgctgc cagggcaaca tacctttaac actgcgttcc agttcttgcg cactgcgtac
    13741 tccccggctg gcgtataaac ggcgcagcaa gggaggcaat tcagcgggca agtctgccgt
    13801 ttcatcgact tcacggcgac gaagttgtat ctgttgtttc acgcgaatta tttaccgctg
    13861 gtcatttttt ggtgttcgtc gaggaattct ttcatctctt tcggcggctg gtaacccgga
    13921 acaagtgtgc cattgctcag cacaactgcc ggagtaccgc taacgccaag ctggacgcca
    13981 agtgcgtaat ggtcggcaat atccacgtcg caactggctg gtgcgacgct tttacctgcc
    14041 atcacatcat caaacgcttt gtttttgtct ttcgcacacc agatagcttt catttctttc
    14101 tcagcatcgc tatccagccc ctggcgcggg aaagcaagat aacgcacggt gatccccagc
    14161 gcgttgtagt ctgccatttg ctcatgcagt ttgtggcagt aaccacaggt aatatcagta
    14221 aacacggtga tgacgtgttt ttcctgcggc gctttataaa cgatcatctc tttttcaagc
    14281 gcattcaact gctttaacag catcttattg gtgacattga ccggagccgt gccactaacg
    14341 tcatacattg gcccctgaat gatatgttta ccatcatcgg tgatgtacaa cacgccgctg
    14401 ttagtcagaa ctgtcttcat gccagctacg ggcgcgggct gaatatcgct gcttttaatg
    14461 cccattttgg ctaacgtttg ctgaattgcc gcgtcatcag cctgagcaaa gcctgaaaac
    14521 gccgctaaca aagtaaacaa cataaaacct ttcttcataa atcttcccgt tcttttcaga
    14581 catcacgccc gcgggtgatg ctgttgatga agttgccgca gacgctcggt agcgacatgc
    14641 gtataaattt gcgtggtgga gagatcgctg tggcccagta gcatctgcac cacgcgtaaa
    14701 tccgcaccat gatttaataa atgagtggca aaagcgtgac gcaacacatg cggtgacagc
    14761 ttttcgctgt cgatacccgc cagcacggca taatgtttaa tacggtgcca gaaggtctgt
    14821 cgcgtcatct gctgcgcacg ctggctggga aacaacacgt caattgacac accattcaac
    14881 agccacggac gcccatgttc cagataggtt tccagccagt aaaccgcctc ttcacctaac
    14941 ggcaccaggc gctctttgtt gcctttacca atgacccgca ctacgccctg acgcaggctg
    15001 atatcactca ttgtcagccc gaccagttca gagacacgca gcccggtagc atacaacact
    15061 tcaagcatgg ctttatcgcg tagctccagt ggctgatcaa ttaatggtgc ctgtaataaa
    15121 cgttcgacct gcgcttcgct taaatctttt ggcaaacgct ggggcaattt cggtgaagcg
    15181 agatgcgcac tgggatcatc ttcacgaaac ttttcgcgat aaagatactg gaacaatcgg
    15241 cgcactgcac tcagcaaacg cgctgagctg gtggctttat acccgccctc cagccgttct
    15301 gccagtaatg cctgtaaatc gtcactttgc gccgtcgcca gcgtcaaccc gcggtgatgc
    15361 aaccactcca ccatcattga cagatcgcga cggtaagcgt tcaacgtatt ttcagccaga
    15421 tttttttcca gccacagagc atcaagaaac tgctcgatgc gtgccagatc ctgtttcact
    15481 tgcgcccctt atggtcactc atttgatcca ttatgcctta ttgtgccgtg actaaagcga
    15541 ttctgataca ctagccgcaa aagccacagc agaatcgaga agcttacgtt atgaatatgg
    15601 gtctttttta cggttccagc acctgttaca ccgaaatggc ggcagaaaaa atccgcgata
    15661 ttatcggccc agaactggtg accttacata acctcaagga cgactccccg aaattaatgg
    15721 agcagtacga tgtgctcatt ctgggtatcc cgacctggga ttttggtgaa atccaggaag
    15781 actgggaagc cgtctgggat cagctcgacg acctgaacct cgaaggtaaa attgttgcgc
    15841 tgtatgggct tggcgatcaa ctgggatacg gcgagtggtt cctcgatgcg ctcggtatgc
    15901 tgcatgacaa actctcgacc aaaggcgtaa agttcgtcgg ctactggcca acggaaggat
    15961 atgaatttac cagcccgaaa ccggtgattg ctgacgggca actgttcgtg ggtctggcgc
    16021 tggatgaaac taaccagtat gaccttagcg acgagcgtat tcagagctgg tgcgagcaaa
    16081 tcctcaacga aatggcagag cattacgcct gatgtcactg acggcttagc acatatgctt
    16141 tgccgtcatc tttatctttg cgtctcttgt tgcaacaata tccgccgtaa atcccgccat
    16201 tcggcttcgt ccatgctgtc ggctgccagc cataaatgtt gccgtttacc gccatcagaa
    16261 cgtaaacgca gcatcatgcc gctcttaatc atccacggtg ctttgacgat gctccactcc
    16321 tgcccttgcc aacgcaaacg cccgtccatc aacaagcgaa tttccccctg gcgagcatta
    16381 atacgccgct ggctgcgaac gcaatcaaac accaccagcg aaagtaacac catccataac
    16441 ggggtgtaac tgagcggcca gggcatgagt aaaataacag cggcaaccag cccatgaatc
    16501 agcaaggaaa gccactgtgc gcgccaggag acgcgcaaat cagattgcca caggaccacg
    16561 ttcccggttc cgtgtctgga tgagtcggac catcatttcc agttctgcat cggccggttt
    16621 accgtgattc atcagccagt taaacaggtc tggatcgtca cattccagca gacgaataaa
    16681 gatgcgtttt tcgtcatcgc ttaagctgtc gtactcatgt tcgaaaaacg gcatgattga
    16741 aatatcgagt tcgcgcatac cacggcggca tgcccaatga atgcgggctt tgttgttaat
    16801 gtccatcttc ttcctgtctc acgaaaatcc agtaccccgc tattgtaacg tgtttttcga
    16861 cttcttttac gggaatatca gtaaacacaa tcgcgatcgc gaaattaatt cacaacaatt
    16921 caagggcttc atttttttgg aagtcgcctc gcagaaggca cagatcgcgt agtgaaagca
    16981 cttgcattgc ctcatagctc ttttaccatt agttattaat acgccgttaa gcaactcagg
    17041 actctattat ggcttttaca ccttttcctc cacgtcagcc tacggcttct gcccgtttac
    17101 cgctgacgct gatgacgctt gatgactggg cgcttgccac cattactggc gcggacagcg
    17161 aaaaatatat gcagggccag gtgacagcag atgtcagcca gatgacagaa aatcagcacc
    17221 tgctcgccgc ccattgcgac gccaaaggta aaatgtggag caatttacgt ctgttccgcg
    17281 acggcgatgg ctttgcgtgg attgaacggc gcagcgtgcg tgaaccgcag ctgactgaac
    17341 tgaaaaaata tgcggtgttc tctaaagtga ccttcgcgcc agacgacgag cgtgtgctgc
    17401 ttggtgttgc cggttttcag gcgcgcgccg cgctggccaa gctctttagt gaactacctt
    17461 cgagagaaaa acaggtagtt aaagaaggcg cgaccacttt gctatggttt gaacacccgg
    17521 cagaacgttt cctgatcgta accgatgaag ctactgccaa tatgctgacc gataaactgc
    17581 gcggtgaagc ggaactgaac aatagccaac agtggctggc attaaacatt gaagcgggtt
    17641 tcccggtgat tgatgccgcg aacagcgggc agtttatccc acaggcgacc aatctccagg
    17701 cgctgggcgg tatcagcttt aagaaaggct gttataccgg acaagagatg gtggcgcgag
    17761 caaaattccg tggtgccaac aaacgcgcgc tctggttgct gacaggtagt gccagccgac
    17821 tgccggaagc tggtgaagat ttagagctga aaatgggcga gaactggcgt cgcaccggta
    17881 cggtgctggc tgcggtaaaa ctggaagatg gtcaggtcgt ggtacaggtc gtcatgaata
    17941 acgatatgga accggacagc atcttccgcg tgcgtgacga tgcgaataca ttgcatatcg
    18001 agccgctgcc gtattcgctc gaagagtaaa tctctgtgtc gcatcaggca tttatcgcct
    18061 gatgcgacgc tggcgcgtct tatcaggcct acaaaggcat acccattacg cctgcccaat
    18121 atacaaatag atcgccagaa agtggcacac actaccgccg agcacgaagc cgtgccagat
    18181 ggcatggttg tatggaatgc gtttgcagac gtagaaaatc accccgagcg aataaaccac
    18241 gccgcctacc gccagtaagg taacgctgcc cgccgcgagc ttaactgcca tttcataaat
    18301 taccaccagc gacagccagc ccatcgccag ataagtcacc agagataaaa ttttgaatcg
    18361 gtgcgcgatg gtcagtttga acagaatccc cagcaatgcc aggctccaga taacaatcat
    18421 caacccgcgc gctaacggag aatccagccc caccagcaaa aacggcgtgt aggttccggc
    18481 aatcaacagg taaatagcgc aatggtcaaa tttcttcagc cacatttttg cccgttgatg
    18541 aggaatggcg tgatagagcg tcgaagcgag gaacagcagg atcatactgc cgccatacag
    18601 gctgtagctg gttatcgctg tggcgctggc attgagatcc accgcctgaa ccagtagcaa
    18661 caccagccca acgataccaa acaccaaccc aatgccgtga ctgacgctgt tggcaatttc
    18721 ctctgccagc gaatatccct gcttaacgag gggcttctga accataactt actccggaga
    18781 aacgtacacg cacatgtata cctctccagc gtaactgaga atagttccag tgaacacctg
    18841 ttagctaaaa taaattctga tttgagacta atctcctaaa aatcatgaaa ttaaatgcga
    18901 aatttcaact aacaggcgtg agttcaattt aaagacattt aaattcaatc acataaaact
    18961 gtgtctgacc gggatagatg tcggcaatga cctttttcag ttcagtcagg gtcatatttt
    19021 cctgctctgc atgtttttcc gtcagcgtat ccagcgttac ggttgaggtt gcggtgactt
    19081 caatcgtgca aaaataaccg tcatcttcaa aacgcccgac acgaagcaca tcacccgttt
    19141 tgaagtgcga ttcagactcg tcgcggatgg tgatggtttt acgcccagcc agaatgtcat
    19201 cctggaaacg ttgaaaaaaa gtgatgtcgt ttggctgcat ggtactattt cctgtaagaa
    19261 ttgactcatc tggagcctat gatagtgaaa aaactcacct taccgaaaga tttcttatgg
    19321 ggcggcgcag ttgccgctca tcaggtcgaa ggcggctgga acaaaggcgg caaagggccg
    19381 agcatttgtg acgttctgac cggtggcgca cacggcgtgc cgcgcgaaat caccaaagaa
    19441 gtcttgccag gaaaatacta tccaaaccat gaagccgttg atttttatgg tcactacaag
    19501 gaagacatca agctatttgc cgaaatgggc ttcaaatgtt ttcgtacatc catcgcctgg
    19561 acgcgcattt ttccaaaagg cgatgaagct cagccaaacg aagaagggct gaagttttac
    19621 gactctctgt tcgatgaatt gctgaaatac aacatcgaac cagtgatcac cctctcccac
    19681 tttgaaatgc cgctgcatct ggtgcagcaa tacggtagct ggaccaaccg taaagtggtt
    19741 gatttctttg tccgtttcgc ggaagtggta tttgaacgct ataagcataa agtcaaatac
    19801 tggatgacct tcaacgaaat taacaaccag cgtaactggc gtgcaccgct gttcggttac
    19861 tgctgctccg gcgtggttta taccgagcat gagaacccgg aagagacgat gtatcaggtg
    19921 ctgcatcacc agtttgtcgc cagcgccctg gcggtgaaag ctgcgcgtcg cattaacccg
    19981 gagatgaaag tcggctgtat gctggcgatg gtgccgctct atccttactc ctgtaacccg
    20041 gacgatgtga tgtttgctca ggagtcgatg cgcgaacgct acgtctttac cgatgtgcag
    20101 ctacgcggct attacccgtc ctatgtgttg aacgagtggg agcgtcgcgg atttaacatc
    20161 aaaatggaag atggcgatct cgatatactg cgcgaaggca cctgcgatta tcttggtttc
    20221 agctattaca tgaccaacgc agtgaaggcc gaaggcggca ccggcgatgc tatctctggt
    20281 tttgaaggca gcgtaccaaa cccgtatgtt aaagcatctg actggggctg gcagattgac
    20341 cctgtgggtc tgcgttacgc gctttgcgaa ctgtatgagc gttaccagaa gccgctgttt
    20401 attgtcgaaa acggttttgg cgcttacgac aaagtggaag aagatggcag catcaacgac
    20461 gactaccgca ttgactacct gcgcgcccat atcgaagaga tgaaaaaagc ggtgacttac
    20521 gatggcgtgg atctgatggg ctacacaccg tggggctgca tcgactgcgt gtcgttcacc
    20581 accgggcagt acagcaaacg ctacggcttt atctatgtga ataaacatga cgacggtact
    20641 ggcgatatgt cgcgttcacg taagaagagc tttaactggt acaaagaggt gattgccagc
    20701 aacggcgaga agctttaatt acccgatgtg cctgatgcgc cccgctcatc aggcctggcg
    20761 ctcctgtcat atattgaact ccaggatttt atatttacgc ttcatccgac acacaaagcg
    20821 cactttatta acaagctaaa aaggcgccga agcgccttta gaagatagtc gaatcagtga
    20881 attactggta ttcgctaatc ggtacgcagg agcagaacag gttacggtcg ccataaacat
    20941 catccagacg tttcacagtc ggccagtatt tgtctgccac gcctgccggg aataccgcaa
    21001 cttcacggct gtacggatgc gcccactcgg cgaccagttc gctctgaatg tgcggcgcgt
    21061 tcaccagcgg gttatcttcc agcggccaga caccggcttt cacctggtca atttctgcgc
    21121 ggatagccag catcgcgtcg ataaagcgat ccagttccac tttgctttca gattcagtcg
    21181 gttcaaccat cagcgtaccc gccaccggga acgacatcgt cggcgcgtgg aaaccgtagt
    21241 cgatcaggcg cttggcaata tccagctcgc tgatgccggt ttcttctttc agcgggcgaa
    21301 tatcgagaat acattcgtgc gccacgcgac cgtcacgacc ggtatacagc accgggaagg
    21361 catcctgcag gcggctggca atatagttgg cgttgaggat tgccacctgg cttgcttttt
    21421 tcagcccttc tgcgcccatc atgcggatgt acatccagct gattggcagg atagaggcgc
    21481 taccgaacgg tgccgcagaa accgcgccct ggcgggttaa catgccttcg atttgcacca
    21541 cgctatgacc cggtacaaac ggagccagat gcgctttcac accgatcggt cccatacccg
    21601 gcccaccacc gccgtgcgga atgcagaaag ttttatgtag gttaaggtgt gaaacgtccg
    21661 caccaataaa gcccggcgag gtgatgccaa cctgggcgtt catgttcgcg ccatcaaggt
    21721 aaacctgacc gccgaactga tgcacgactt cacacacttc acggatcgtt tcttcataca
    21781 cgccgtgggt ggaaggatag gtcaccatga tacaggagag gttatcgccc acctgttccg
    21841 ctttcgcgcg cagatcagcc agatcgatgt tgccgttttt atcacacgcc acaaccacca
    21901 cctgcattcc tgccatatgt gcagaagccg ggttagtgcc gtgcgcagag gcagggatca
    21961 ggcagatatc gcgatgccct tcgttgcggc tttcatgata atgacgaatc gccagcaggc
    22021 ccgcgtattc tccctgagcg ccagagttcg gctgcataca gacggcgtcg taaccggtca
    22081 gtttcaccag ccagtcagcc agctgcgcga tcatctgctg ataaccttcg gcctgctccg
    22141 gcgggcagaa cggatgcagt tcggcaaatt ccggccaggt gatggggatc atctcggcgg
    22201 cggcgttcag tttcatggtg caggaaccca gcgggatcat cgcctgattc agcgccagat
    22261 ctttacgctc cagcgagtgc atatagcgca tcatttcggt ttcgctgtgg tagcgattaa
    22321 acaccggatg ggtgaggatt tcgtcgtcgc gcagcatcgc aggctggata gagcggctgt
    22381 cgtgagccac gtctttgtcc agcgtgtcga tgtccagccc gtgattatcc cccagtagta
    22441 cgctgaaaag ctgcatcacg ttttcgcgcg tggtggtttc atcaagggtg atcccaaccg
    22501 cgttcaggat atcgctacgc aggttgattt cagccgcttc ggcacgcgcc agtacgcccg
    22561 ccttgtcggc cacttccaca cacagagtgt cgaaatagtg cgcatggcgc agcttcagac
    22621 ctttttgttg caggcctgcc gccaggatat cggtcagacg gtgaatgcgg ttagcgatac
    22681 gtttcaggcc aatcgggccg tgataaacgg catacaggct ggcgatgttt gccagcagca
    22741 cctgggaagt acaaatgttg gagttcgctt tctcacgacg aatatgttgc tcgcgagtct
    22801 gcatcgccat gcgcagcgcg gtattgccag ctgcatcttt cgatacaccg ataatacggc
    22861 ctggcattga gcgtttgtat tcgtctttcg ccgcaaagaa tgccgcgtgt gggccaccgt
    22921 aacccatcgg tacgccaaag cgttgcgccg aaccaaaaac aatatccgcg ccctgtttac
    22981 ccggcgcagt taacagcacc agcgccataa tatcagcggc aacgctgacc acaattttgc
    23041 gtgatttcag ttcgctgata agcgcagtgt agtcgtgaat ttcaccggta gtgccaacct
    23101 gctgtaacag cacgccgaag acgtcctgat ggtcgagcac tttttgcgca tcatcgacaa
    23161 tcacttcaaa accaaaggtt tcggcacgag tacggaccac atccagcgtt tgcggatgaa
    23221 cgtcagaagc aacaaagaag cggttggcat ttttcagttt gctgacgcgt ttcgccatcg
    23281 ccattgcttc ggcggcagcg gtggcttcgt caagaagaga agcagaggcc atatccagcc
    23341 cggttaaatc cagcgttacc tgctggaagt tgagcagtgc ttcaaggcgg ccctgggaga
    23401 cttcaggttg atacggagta tacgcggtat accagcccgg attttccagc atgttacgca
    23461 ggataaccgg cggtagctgc acggcggtgt aacccatgcc gatgtaagac gtgaagcgtt
    23521 tattgcgact ggcaatagcc ttgagttccg ccagcgcggc gtattcggtc gccggtgcgc
    23581 caacctgcgg cggggtcgca agctgaatat ctttcggcac aatctggccg gtcagcgcgt
    23641 ttaacgattg tgcaccaacg gcattcagca tttcttgctg ttgcgcggcg tccggtccga
    23701 tatggcgttc aataaaagcg ccgctatttt caagctggct taacgtctgt gtcatgagcg
    23761 atggttcctg aaacgtgcag tgaattgtga acctctctcc ttacgaagag agtgagggtg
    23821 aggcgtaaat ttcctcaccc agatcctctc ccgcagaaga ggaataaagc cgttactcgt
    23881 cttctaacaa tgcttcgtat gcggtcgcat ccagcagtga ttccagttcg ctttcatcgc
    23941 tggctttgat tttgaaaatc cagccgcctg catacggttc gctgttcacc agttccgggg
    24001 aatcgctcag tacgtcgttt accgccacga tttcaccgct tactggcgca taaatgtctg
    24061 acgccgcttt taccgattcg gcaaccgcgc agtcatcgcc cgcgctaacc gtcgcgccca
    24121 cttccggcag gtcaacaaac accatatcgc ccaacaactc ctgagcgtgt tcggtgatgc
    24181 caacggtata agtgccgtcg gcttctttac gcagccattc gtgttctttg ctgtatttca
    24241 gttctgctgg tacgttgctc atcaatcaat ctccaaaaaa gtaaatcacg cgacggcttt
    24301 gccgttacgc acaaaaacag gtttcgtcac tttaaccggc atttcacggt tgcgaatttg
    24361 cacaatcgct gtttcaccaa taccttccgg cacgcgcgcc agcgcaatgc tgtaacccag
    24421 cgtcggggag aaagtcccgc tggtgataat gccttcatgc tggttgccct gcgcatcggt
    24481 aaaacgtacc ggcagttcat tacgcagcac acctttttcg gtcatcacca gaccaaccag
    24541 tttttctgta ccatgctcac gctgcgcttc cagggcttca cgaccgatga agtcacgatc
    24601 tgccggttcc caggcgatag tccagcccat attggcggct aaaggagaaa tagtttcgtc
    24661 catctcctgg ctatacagat tcatgcccgc ttccagacgc agcgtgtcac gcgcccccag
    24721 gccacatggc ttaacgcccg cttccaccag cgcacgccag aaatccgccg ctttttcatt
    24781 gggcagcgca atttcgtagc ccgcttcacc ggtgtaacca gtggtggcaa taaacagatc
    24841 gcccgcctgc acgccaaaga acggtttcat cccttccacc gcctgacgct gggcgtcatt
    24901 aaacagtgtg gcagctttta cctgcgcatt cggcccttgt acggcaatca tggaaaggtc
    24961 atcacgaacg gtaatttcga tgccgaaagg ttcagcgtgt tgggtaatcc aggagaggtc
    25021 tttttcgcgg gtggcggagt taacaacgag gcggaagaaa tcttcagtaa agtagtagac
    25081 gatgagatca tctatcacac cgccagaggc attcaacatc cccgagtaaa gggctttgcc
    25141 gcttttggtg agcttcgcca catcgttcgc cagcagataa cgcagaaact cccgggtgcg
    25201 gctgccgcga agatcgacga tggtcatatg tgacacatca aacattccgg catcggtacg
    25261 tactgcatga tgttcgtcga tttgcgaacc gtaatgcagc ggcatcatcc agccgtggaa
    25321 atccaccatg cgggcgccgc aaagcgtgtg ttgttcgtac aaaggagtct gttgtgccat
    25381 cttgtcctca ttgaataagc ggggctgaca acttttccat ggtgaaatta tcaccacgaa
    25441 acccagcatc ggagccactc ccggtcccca acgcaatcgt tctcttttgc ctgaacttac
    25501 caccgaaaca gactgttaac cataaggtaa aattgatcat cacattagct tatggttaaa
    25561 aaatgcaaaa atcgcgacag aataaaaaac caaaaaacac accagtttct atacaaagat
    25621 gatgtgatga gaaagtcaat ttgaataaga caatattaag agctaaaaaa atgtcaaaaa
    25681 acactaaatc aaaaaatcat ggcattagaa aatataatgc gaaaacggag gtgaaattag
    25741 tttatttcaa atgaggaaaa tctcccggcg aaaaaaccgg gagatgaaag tgtgatgcgt
    25801 atcaaataaa cagcagaaga gaaattttta acgcagccat tcaggcaaat cgtttaatcc
    25861 catcgcctga cggataagtt gtggcttaac gccaggaagc gtgtcggcca gtttcaaacc
    25921 aatatcacgc agcagttttt tcgccggatt ggtaccggaa aacagatcgc ggaatccctg
    25981 cataccagcc agcatcagcg ccgcgctgtg cttgcggcta cgttcatagc ggcgcaggta
    26041 aatgtactgc ccgatgtctt taccctgacg atgcaaccgt ttcagttcag caatcagctc
    26101 tgcggcgtcc ataaagccga gattaacgcc ctgccccgcc agtgggtgaa tggtatgcgc
    26161 ggcgtcgccc accagcgcca gacgatgtgc ggcaaactgg cgcgcataac gccccgtcag
    26221 cgggaacacc tgacgctcgc tctcaacctt gcataagccc aggcgattat caaaagcgat
    26281 atttaacgcg cgattaaatt cgtcttcccc tgcctgctgc atccgctgcg cttcctctgg
    26341 cgacagcgac cagacaatcg agcaaagatg cggatcgcta agcggtaaaa aggccagaat
    26401 gccttcgccg tggaaaacct gccgcgccac cgcatcatgc ggttcttccg tgcgtatggt
    26461 cgctaccagc gcgtgatgct gataatccca gaaagtcagc ggaatatcgg ctttgttgcg
    26521 caaccaggaa ttagcgccgt ccgcgccaat caccagacgc gccgttaaca tactgccatc
    26581 tttcagcgtc aggaaggttt cgttttctcc ccaggcgacc tgctgtaatt ctgcgggggc
    26641 caacagagtg atatctgacg actgctgcgc tttgttccac agcgcgtagt gaatcactga
    26701 gttttcaacg atatgcccaa gatggctata gcccatgctt tgatcgtcaa acgaaatgtg
    26761 accaaagctg tctttgtccc acacttccat accgtgataa cagctggctc ggcgagagag
    26821 aatgtcctgc cagacgccaa gacgggtgag taatttttcg ctggcggcat tgatagccga
    26881 aacgcgcagt tgtggtggtg catccgccgc cagaggttcc ggtacgcgct gctccagtac
    26941 ggcaacgcgt aagccgctac cctgtaagcc gcaggcaacc gccagcccca ccatgccgcc
    27001 gccaacaatg gctacatcaa cactttgcat tgtttattcc ttaaaaccgc cttcaacgcg
    27061 ccacccaacc gagggtgcgc tgcgccagca catcgcgtgc cggggtgaat aattccatcg
    27121 tcatcagccc gatgttgcgc ccgacaacca gcggcgtcca acggttggca aaaagatgta
    27181 caaggctgtc cgtgacgcca atggttgctt cgcgatcgct ctgtcgacgc tgctgataac
    27241 gacacaatac gccgtaatca cccatgtctt ctccgcgctc ctgcgcctga gtcagggttt
    27301 ccgcaagact catgacatct cgcataccga ggttgaaccc ttgcccggca atcgggtgca
    27361 gggtttgcgc cgcattgccc accagcacgg tacgatgggt aatagatctg gcggcgtggg
    27421 ttaacgccag cggataagca ctgcgtttac cagcgtgggt aattttccca agtcgccagc
    27481 caaaggccga ctggagttca cggcaaaact tctcgtcgct ccacgacagc acctcttcgc
    27541 gccgttccag tggatgacac cagaccagcg aacagcgtcc gtcagacatc ggcaacatcg
    27601 ccagcgggcc atgttgcgta aagcgttcaa acgcgcggcc ttcgtgcgca acagaagtcg
    27661 caacgttggc aatcactgcc agttgttcgt aaggctcctg ctgccagtca acgccgcaca
    27721 cagtggctaa cgctgaatgg gtgccatcag cggctaccag cacgcggccc gtcagcgtct
    27781 cgccacactc cagcgtcact tcaacatgac tctgagtacg ggcaacgtta gccacgcgat
    27841 ccgggcaatg cagcgttacg ccaggtgctt tgcgcagcaa tgcaaacagc cgttgcccga
    27901 cattgtgcaa ttcgacaacc tgccccagcg ccgccagttg gtaatcttct gcggcgaggg
    27961 tgacgaatcc ggcatgacca cgatcgctga catgcacggt agtgatggcg gttgcgcaat
    28021 cagccagaga ttgccagacg ccgatgcgcg ccagttgctg acaggtcccc gccgctagag
    28081 caatagctcg tccatcaaag cccggatgag catgtgactc tggcgcggtc gcttcaatca
    28141 aatgtaccgg cagcgctccg tgacttaacc gggaaatagc cagcgccagc gtcgcgcccg
    28201 ccatgccgcc accgacgatg attacgctca ttgctttctc gcagctgcca tcaacgcttc
    28261 gatttcttcc ggctttttca ccacgctggc ggtgaggttt tcgttaccgg tttcggtaat
    28321 cacaatgtcg tcttcaatac gaatgccgat accgcgatat tgttctggca cttctgcatc
    28381 cggcgcaata tacagccctg gctctacggt cagtaccatg cccggttcca gaatgcgcga
    28441 gcgatcctga ccataaacac ccacgtcatg gacatccagt cctaaccagt ggctaaggcc
    28501 atgcataaag aaaggacgat gggcgttctg agcgatcagt tcatcaacat cacctttcag
    28561 gatgccgagt tttaccaggc cgctaaccat gatgcgcacc acttcaccgg tgacttcctg
    28621 aatggaagtt cccggacgat acaggcgcag gctggtttcg agagactcca gcacaatgtc
    28681 gtagatttca cgctgagcct gagtgaattt gccgttgacc gggaaggtgc gggtaatatc
    28741 gccagcgtaa cctttgtatt cgcaacccgc gtcaatcaac accagatcgc cgtcgcgcag
    28801 ttcacactcg ttttcggtgt aatgcagaat gcagccgttt tcaccgctgc cgacaatggt
    28861 gttataggaa ggatagcgcg cgccgtggcg gttaaattcg tggtgaattt cgccttccag
    28921 gtggtactcg aacattcccg gacggcattt ttccatcgcc cgggtatgcg ccagggcggt
    28981 gatttcaccc gcgcggcgaa gtacggcaat ctcttccggc gatttgaaca ggcgcatttc
    29041 atgaacaatg ggacgccagt cgatcatcgt tgccggcgcg gtgagatttt gccgcgaacc
    29101 tttacgcagt ttctcaagcg cgctgttcac gatttcatca gcatatgcat attcgccctg
    29161 ggcgtggtaa accacatcca gaccattaag tagttgataa agttgctgat taatttcgct
    29221 gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg gcatcctgac ctaaacgacg
    29281 gccaaaccag atctccgccg tcaggtcgcg aacgcggtta aacagaacgc tgtggttatg
    29341 agtgtcatcg cttttaatca gcaccagcac cgcttccggt tcgttaaagc cggtgaagta
    29401 ccagaagtca ctgttctgac gataggggta ttcgctgtcg gcgctacgtg ttacttctgg
    29461 tgcagcaaaa atcagcgcgg cgctgccggg ttgcatttgc tccaccaggg cctgacggcg
    29521 acgctgaaac tcttgccggg atatctcact cataacactc tccttacgtt ttttgttttt
    29581 agtgtagagt cggtttttgt acttctggcg cggtcggttg cggatgagta aaggtgtcgt
    29641 ggcataacag cgcggcaaca cggacgtact cgatgatctc ttcaagcgac atttcaagct
    29701 cttcctgatc ttcgtcttcg tcgtagccca gctgcgcgat gttacgcaga tcgtcgatgg
    29761 cttcaccggt ttctccggtc actttgtcca gcttcggttg cgtaacgcca agaccaagca
    29821 ggaagtgatt gacccaacct gccagtgcat cagcccgatc gaaaacgctg acatcatcgc
    29881 catcaggcag ataaagctga aaaaggaagc cgtcatcctg cagggcatcg ctggtggcag
    29941 agtgcatttt acgcagtgcc tgtgccagct catgaccgaa agccatgcct tcgttcgtca
    30001 ggtcgtgaag tagcggcagc catgagctgt catcgttacc gccacatatc atcccactga
    30061 ttaaaccatg catctcagca ggggtcagac ccgtcccttg ttggttcaga tactggttca
    30121 tttcgttgta accaggcatt tcgttctgta tagacataag cattcgtcgt caaagggagg
    30181 aatattcatg atatgctacc actttgggcc ctggtggacc agaaaagggc ttgtctcttc
    30241 tcatcagggt agctatagtg tcgccccttc gcagaccatg ggtctaaaga cgaaggcagc
    30301 gcagtcaatc agcaggaagg tggcatgtct gcacaacccg tcgatatcca aatttttggc
    30361 cgttcactgc gtgtgaactg cccgcctgac caaagggatg cgttgaatca ggcagcggac
    30421 gatctgaacc aacggttgca agatctgaaa gaacgcacta gagtcacaaa tactgaacag
    30481 ttggtcttca ttgccgcatt gaatatcagc tatgagttag cgcaagaaaa agcgaagact
    30541 cgtgactacg cggcaagtat ggaacagcgt attcggatgc tgcagcagac catagaacaa
    30601 gcgttacttg aacaaggtcg catcaccgaa aaaactaacc aaaactttga atgacacttt
    30661 tcggtttact gtggtagagt aaccgtgaag acaaaatttc tctgagatgt tcgcaagcgg
    30721 gccagtcccc tgagccgata tttcatacca caagaatgtg gcgctccgcg gttggtgagc
    30781 atgctcggtc cgtccgagaa gccttaaaac tgcgacgaca cattcacctt gaaccaaggg
    30841 ttcaagggtt acagcctgcg gcggcatctc ggagattccc ttcttatctg gcaccagcca
    30901 tgacgcatct accagaactc ccactgatat tatcccgaca agaaatccgc aaaatgattc
    30961 ggcaacgtcg tcgtgcgtta acgccggaac aacagcagga aatgggtcaa caagccgcta
    31021 cccggatgat gacttatccc ccggtggtga tggcacatac ggtcgctgta ttcctctctt
    31081 ttgatggcga actcgacacc cagccactca tagaacaact ctggcgcgcc ggtaagcgcg
    31141 tatatcttcc agttttgcat ccctttagtg ccggtaattt gctgttcctg aattaccatc
    31201 cgcaaagtga actggtgatg aacaggttga agatccatga gccaaaactg gatgtgcgtg
    31261 acgtgctacc cctttcccga ttagacgtgt tgatcacacc gctggtcgcc tttgatgagt
    31321 acggtcagcg cctgggaatg ggcggaggtt tttatgatcg gaccttacaa aactggcagc
    31381 actataaaat gcaaccggtg ggttatgcgc atgattgtca gttggtggaa aaactccccg
    31441 ttgaagagtg ggatatccct cttcctgcgg tggttacacc gtcgaaaatc tgggagtggt
    31501 gagggcgaca cacctgcatc gccctgattg acatcgttga ttctttaacc taatttagtg
    31561 agtaagggta agggaggatt gctcctcccc tgtgactgac tgttaataag cgctgaaact
    31621 tatgagtaac agtacaatca gtatgatgac aagtcgcatc ataacccttc tccttcaagc
    31681 cctcgcttcg gtgagggctt taccgttaca gccccatgct gccctgctat cgtaaactct
    31741 ctttaaataa gcacaacgca ttgatctacc tttaaatttt ttcttcgagc gcactcgtaa
    31801 aacgtcatgt aagacagaca cacattgcag ggataaggga ttaattacgg cagtaaacgt
    31861 gggcgatgaa gtgtctatca aatgatcggt tcagggcgat acatcccgca tcgccctgat
    31921 tgacatcgtt gattctttga cctaatttag tgagtaaggg taagggagga tgactcctcc
    31981 cctgagactg actgttaata agctgggaaa cttatgagta acagtacaat cagtatgatg
    32041 acaagtcgca tcataaccct tctccttcaa gccctcgctt cggtgagggc tttaccgtta
    32101 cagccctgtg ctgtcctgcc atcgtaaatc cccattaaat aaacacaacg cattgatctg
    32161 actgtgattt attttctgga gcagactcgc aaagtagaat gcgcaacgcg gcaacggtgt
    32221 ggagaaggga taaaaaaacg ggcaagtcag tgacctgccc gttgattttc agagaagggg
    32281 aattagtaca gcagacgggc gcgaatggta cccggaatag ctttcattgc ctgcagcgct
    32341 ttttcggcaa cgtcttcgtc ggcttcaata tcaataacca cataacccat ctgggcggaa
    32401 gtttgtagat attgcgcggc gatgttgacg ccctgctcgg cgaagatttt gttcagcgca
    32461 gttagcacgc ccggacggtt ttcgtggatg tgcatcagac gacgcccacc gtgcagtggc
    32521 agcgagactt ccgggaagtt caccgcagag agcgttgagc cattgtcaga atacttgatc
    32581 aatttacccg caacttccag gccgatattc tcctgcgctt cctgagtcga accgccaatg
    32641 tgtggcgtca gaaggacatt gtcgaattca cacagcggag aggtaaatgg atcgctattg
    32701 gtcgctggtt ccgtcgggaa tacgtcgatt gccgcccccg ccagatgttt gctcgccagc
    32761 gcatcacaca gcgccggaat atccaccaca gtaccgcgcg aggcattaat cagcagcgag
    32821 ccgggcttca ttagtgaaat ctctttcgca cccatcatat ttttggtgga cggattctct
    32881 ggtacatgca gactcaccac atcgctcata ttcagcaggt cagaaagatg ctgtacctga
    32941 gtggcgttgc ccagcggcag tttattttca atatcataaa agtaaacata cattcccagc
    33001 gattcagcca gaatgcccaa ttgcgtacca atatgaccgt agccgatgat acccagcttt
    33061 ttgccgcgcg cttcaaaaga ccccgccgcc agtttgttcc acacgccacg gtgcgcttta
    33121 gcattggctt ccggcacgcc gcgcaatagc agcagcagtt cgccaatcac cagctccgca
    33181 acagagcgcg tatttgagaa cggtgcgtta aataccggga tcccgcgctt tgccgccgca
    33241 tccagatcaa cctggtttgt tccgatacag aaacagccaa tagcgaccag cttttctgcg
    33301 gcgttgatca cgtcttcagt cagatgggta cgggatcgca ggccgatgaa gtgggcatcg
    33361 cggatggatt cttttaattg ttcatcatcc agcgcgcctt tgtgaaattc gatgttggtg
    33421 taaccagctg cacgaaggct ttccagcgcc ttttggtgca cgccttctac cagcagaaac
    33481 ttaatcttgt ctttctccag cgataccttt gccatttacc caatcctgtc ttttgaaatg
    33541 ttgtgtgcgg atttgcgtcc gcctttcaac atatcaaaaa ataatattgc ggcaatatga
    33601 acgtttgcgt cgcgatgttg aggaaatatc acgcaaaggc cattcttgga tgaatatgct
    33661 ggtttagagg atttatttag agcaatcgac aatagccagg taaaaacgtg acacatgtca
    33721 ccaaatttaa tgaagagaat ttttttaacg ggggaggttc ccccgtcaga tcatttcaca
    33781 atggttttga caccgtcagg tgtgccaatc agcgcaacgt ccgcgccacg gttagcaaac
    33841 aagccaacag tcaccacgcc aggaatcgca tttatggcgt tttccatcgc tatcgggtca
    33901 aggatttcca tgccgtggac gtcgaggatc acgttgccat tatcggtcac cacgccctga
    33961 cggtattccg gacgaccgcc cagtttcacc agctggcgcg ccactgcact acgtgccatc
    34021 ggaataactt ctactggcag cgggaatttg cccagaatat caacctgctt ggaggcgtcg
    34081 gcaatacaga taaatttttc cgcaaccgaa gcaatgattt tttcacgggt cagcgccgcg
    34141 ccgccgcctt tgatcatttg catgtggccg ttgatttcat ctgcgccatc aacgtagatg
    34201 ccaaggctgt cgacttcgtt gagatcaaaa acgtgaatgc cgaggctttt cagtttttca
    34261 gtggaagcat ctgaactgga aacggcccct tcaatctggc ctttcatcgt gccgagcgcg
    34321 tcaataaagt gtgcggcggt ggaacctgtg cctacaccaa caatggtgcc gggctgaaca
    34381 tactgaagtg ccgcccatcc tactgctttt ttcaattcat cctgcgtcat gatcgtttcg
    34441 cctgtggtat gaaatttcac acgcattata tacaaaaaaa gcgattcaga ccccgttggc
    34501 aagccgcatg gttaactcat ccataaaata tcgcgcaatg gcgggcatcc cctttcgccc
    34561 ggcaaataaa gcatacaatg gtctgggtat gccgctccac ggtgcaaaca ggcgcaccag
    34621 ttcaccgttc gcaagcccct gtttacaggc aaattgaggc aataacgcca cgccattcaa
    34681 cacaaccagg gcgcgaactc gctgagcgaa atgcattagc gcaaatccct gatcttttaa
    34741 gctgcgcgtt taaccacgaa tacaaatgtt acccgccgac ggcttcggta tatgcaacct
    34801 gacacaaaat tgtgtcatag tgcaggaaaa agcatttacc aggagcagac aacagcaatg
    34861 aaacgcccgg actacagaac attacaggca ctggatgcgg tgatacgtga acgaggattt
    34921 gagcgcgcgg cacaaaagct gtgcattaca caatcagccg tctcacagcg cattaagcaa
    34981 ctggaaaata tgttcgggca gccgctgttg gtgcgtaccg taccgccgcg cccaacggaa
    35041 caaggtcaaa aactgctggc actgctgcgc caggtggagt tgctggaaga agagtggctg
    35101 ggcgatgaac aaaccggttc gactccgctg ctgctttcac tggcggtcaa cgccgacagt
    35161 ctggcgacgt ggttgcttcc ggcactggct cctgtgttgg ctgattcgcc tatccgcctc
    35221 aacttgcagg tagaagatga aacccgcact caggaacgtc tgcgccgcgg cgaagtggtc
    35281 ggcgcggtga gtattcaaca tcaggcgctg ccgagttgtc ttgtcgataa acttggtgcg
    35341 ctcgactatc tgttcgtcag ctcaaaaccc tttgccgaaa aatatttccc taacggcgta
    35401 acgcgttcgg cattactgaa agcgccagtg gtcgcgtttg accatcttga cgatatgcac
    35461 caggcctttt tgcagcaaaa cttcgatctg cctccaggca gcgtgccctg ccatatcgtt
    35521 aattcttcag aagcgttcgt acaacttgct cgccagggca ccacctgctg tatgatcccg
    35581 cacctgcaaa tcgagaaaga gctggccagc ggtgaactga ttgacttaac gcctgggcta
    35641 tttcaacgac ggatgctcta ctggcaccgc tttgctcctg aaagccgcat gatgcgtaaa
    35701 gtcactgatg cgttactcga ttatggtcac aaagtccttc gtcaggatta atccatcaaa
    35761 taatgcctga tagcacatat caggcgttgt cctcacttct ttttgtattc cttgaatcac
    35821 atcacaaaat agacaaatct caggcggcaa aaaacgacgt ctgaatgcat ttttttgctg
    35881 gcgacaaacc cacgtaaaaa gctcaccgta ggcgcaaata ccctcatttt gattgcgttt
    35941 tacggagcaa ataatgtcta acgtgcagga gtggcaacag cttgccaaca aggaattgag
    36001 ccgtcgggag aaaactgtcg actcgctggt tcagcaaacc gcggaaggga tcgccatcaa
    36061 gccgctgtat accgaagccg atctcgataa tctggaggtg acaggtaccc ttcctggttt
    36121 gccgccctac gttcgtggcc cgcgtgccac tatgtatacc gcccaaccgt ggaccatccg
    36181 tcagtatgct ggtttttcaa cagcaaaaga gtccaacgct ttttatcgcc gtaacctggc
    36241 cgccgggcaa aaaggtcttt ccgttgcgtt tgaccttgcc acccaccgag gctacgactc
    36301 cgataacccg cgcgtggcgg gcgacgtcgg caaagcgggc gtcgctatcg acaccgtgga
    36361 agatatgaaa gtcctgttcg accagatccc gctggataaa atgtcggttt cgatgaccat
    36421 gaatggcgca gtgctaccag tactggcgtt ttatatcgtc gccgcggaag agcaaggtgt
    36481 tacacctgat aaactgaccg gcaccattca gaacgatatc ctcaaagagt acctctgccg
    36541 caacacctat atttacccgc caaaaccgtc aatgcgcatt atcgccgaca tcatcgcctg
    36601 gtgttccggc aacatgccgc gatttaatac catcagtatc agcggttacc acatgggtga
    36661 agcgggtgcc aactgcgtgc agcaggtagc atttacgctc gctgatggga ttgagtacat
    36721 caaagcagca atctctgccg gactgaaaat tgatgacttc gctcctcgcc tgtcgttctt
    36781 cttcggcatc ggcatggatc tgtttatgaa cgtcgccatg ttgcgtgcgg cacgttattt
    36841 atggagcgaa gcggtcagtg gatttggcgc acaggacccg aaatcactgg cgctgcgtac
    36901 ccactgccag acctcaggct ggagcctgac tgaacaggat ccgtataaca acgttatccg
    36961 caccaccatt gaagcactgg ctgcgacgct gggcggtact cagtcactgc ataccaacgc
    37021 ctttgacgaa gcgcttggtt tgcctaccga tttctcagca cgcattgccc gcaacaccca
    37081 gatcatcatc caggaagaat cagaactctg ccgcaccgtc gatccactgg ccggatccta
    37141 ttacattgag tcgctgaccg atcaaatcgt caaacaagcc agagctatta tccaacagat
    37201 cgacgaagcc ggtggcatgg caaaagcgat cgaagcaggt ctgccaaaac gaatgatcga
    37261 agaggcctca gcgcgcgaac agtcgcttat cgaccagggc aaacgtgtca tcgttggtgt
    37321 caacaagtac aaactggatc acgaagacga aaccgatgta cttgagatcg acaacgtgat
    37381 ggtgcgtaac gagcaaattg cttcgctgga acgcattcgc gccacccgtg ataatgccgc
    37441 cgtaaccgcc gcgttgaacg ccctgactca cgccgcacag cataacgaaa acctgctggc
    37501 tgccgctgtt aatgccgctc gcgttcgcgc caccctgggt gaaatttccg atgcgctgga
    37561 agccgctttc gaccgttatc tggtgccaag ccagtgtgtt accggcgtga ttgcgcaaag
    37621 ctatcatcaa tctgagaaat cggcctccga gttcgatgcc attgttgcgc aaacggagca
    37681 gttccttgcc gacaatggtc gtcgccagcg cctattccga tctcggtttc gacgtagatt
    37741 taagcccgat gttctctaca cctgaagaga tcgcccgcct ggcagtagaa aatgacgttc
    37801 acgtagtggg cgcatcctca ctggctgccg gtcataaaac gctgatcccg gaactggtcg
    37861 aagcgctgaa aaaatgggga cgcgaagata tctgcgtagt cgccggtggc gtcattccac
    37921 cgcaggatta cgccttcctg caagagcgcg gcgtggcggc gatttatggt ccaggtacac
    37981 ctatgctcga cagtgtgcgc gacgtactga atctgataag ccagcatcat gattaatgaa
    38041 gccacgctgg cagaaagtat tcgccgctta cgtcagggtg agcgtgccac actcgcccag
    38101 gccatgacgc tggtggaaag ccgtcactcg cgtcatcagg cgctaagtac gcaactgctt
    38161 gatgccatta tgccgtactg cggtaacgcc ctgcgactgg gcgttaccgg cacacccggc
    38221 gcggggaaaa gtacctttct tgaagccttt ggcatgttgt tgattcgtga tggattaaag
    38281 gttgcggtta ttgcggtcga tcccagcagt ccggtcactg gcggtagcat tctcggggat
    38341 aaaacccgca tgaatgacct ggcgcgtgcc gagacggcgt ttattcgccc ggtaccatcc
    38401 tccggtcatc tgggcggtgc cagtcagcga gcgcgggaat taatgctgtt atgcgaagcg
    38461 gcgggttatg acgtagtgat tgtcgaaacg gttggcgtcg ggcagtcgga aacggaagtc
    38521 gcccgcatgg tggactgttt tatctcgttg caaattgccg gtggcggcga tgatctgcag
    38581 ggcattaaaa aagggctgat ggaagtggct gatctgatcg ttatcaacaa agacgatggc
    38641 gataaccata ccaatgtcgc cattgcccgg catatgtacg agagtgccct gcatattctg
    38701 cgacgtaaat acgacgaatg gcagccacgg gttctgactt gtagcgcact ggaaaaacgt
    38761 ggaatcgatg agatctggca cgccatcatc gacttcaaaa ccgcgctaac tgccagtggt
    38821 cgtttacaac aagtgcggca acaacaatcg gtggaatagc tgcgtaagca gaccgaagaa
    38881 gaagtactga atcacctgtt cgcgaatgaa gatttcgatc gctattaccg ccagacgctt
    38941 ttagcggtca aaaacaatac gctctcaccg cgcaccggcc tgcggcagct cagtgaattt
    39001 atccagacgc aatattttga ttaaaggaat ttttatgtct tatcagtatg tgaacgttgc
    39061 cactatcaac aaagtggcgg tcattgagtt taactatggc cgaaaactta atgccttaag
    39121 taaagtcttt attgatgatc ttatgcaggc gttaagcgat ctcaaccggc cggaaattcg
    39181 ctgtatcatt ttgcgcgcgc cgagtggatc caaagtcttc tccgcaggtc acgatattca
    39241 cgaactgccg tctggcggtc gcgatccgct ctcctatgat gatccgttgc gtcaaatcac
    39301 ccgcatgatc caaaaattcc cgaaaccgat catttcgatg gtggaaggta gtgtttgggg
    39361 tggcgcattt gaaatgatca tgagttccga tctgatcatc gccgccagta cctcaacctt
    39421 ctcaatgacg cctgtaaacc tcggcgtccc gtataacctg gtcggcattc acaacctgac
    39481 ccgcgacgcg ggcttccaca ttgtcaaaga gctgattttt accgcttcgc caatcaccgc
    39541 ccagcgcgcg ctggctgtcg gcatcctcaa ccatgttgtg gaagtggaag aactggaaga
    39601 tttcacctta caaatggcgc accacatctc tgagaaagcg ccgttagcca ttgccgttat
    39661 caaagaagag ctgcgtgtac tgggcgaagc acacaccatg aactccgatg aatttgaacg
    39721 tattcagggg atgcgccgcg cggtttatga cagcgaagat taccaggaag ggatgaacgc
    39781 tttcctcgaa aaacgtaaac ctaatttcgt tggtcattaa tccctgcgaa cgaaggagta
    39841 aaaatggaaa ctcagtggac aaggatgacc gccgatgaag cggcagaaat tatccagcat
    39901 aacgacatgg tggcatttag cggctttacc ccagcgggtt cgccgaaagc cctacccacc
    39961 gcgattgccc gcagagctaa cgaacagcat gaggccaaaa agccgtatca aattcgcctg
    40021 ctgacggggg cgtcaatcag cgccgccgct gacgatgtac tttctgacgc cgatgctgtt
    40081 tcctggcgtg cgccatatca aacatcgtcc ggtttacgta aaaagatcaa tcagggcgcg
    40141 gtgagtttcg ttgacctgca tttgagcgaa gtggcgcaaa tggtcaatta cggtttcttc
    40201 ggcgacattg atgttgccgt cattgaagca tcggcactgg caccggatgg tcgagtctgg
    40261 ttaaccagcg ggatcggtaa tgcgccgacc tggctgctgc gggcgaagaa agtgatcatt
    40321 gaactcaatc actatcacga tccgcgcgtt gcagaactgg cggatattgt gattcctggc
    40381 gcgccaccgc ggcgcaatag cgtgtcgatc ttccatgcaa tggatcgcgt cggtacccgc
    40441 tatgtgcaaa tcgatccgaa aaagattgtc gccgtcgtgg aaaccaactt gcccgacgcc
    40501 ggtaatatgc tggataagca aaatcccatg tgccagcaga ttgccgataa cgtggtcacg
    40561 ttcttgttgc aggaaatggc gcatgggcgt attccgccgg aatttctgcc gctgcaaagt
    40621 ggcgtgggca atatcaataa tgcggtaatg gcgcgtctgg gggaaaaccc ggaaattcct
    40681 ccgtttatga tgtattcgga agtgctacag gaatcggtgg tgcatttact ggaaaccggc
    40741 aaaatcagcg gggccagcgc ctccagtctg acaatctcgg ccgattccct gcgcaagatt
    40801 tacgacaata tggattactt tgccagccgc attgtgttgc gtccgcagga gatttccaat
    40861 aacccggaaa tcatccgtcg tctgggcgtc atcgctctga acgtcggcct ggagtttgat
    40921 atttacgggc atgccaactc aacgcacgta gccggggtcg atctgatgaa cggcatcggc
    40981 ggcagcggtg attttgaacg caacgcgtat ctgtcgatct ttatggcccc gtcgattgct
    41041 aaagaaggca agatctcaac cgtcgtgcca atgtgcagcc atgttgatca cagcgaacac
    41101 agcgtcaaag tgatcatcac cgaacaaggg atcgccgatc tgcgcggtct ttccccgctt
    41161 caacgcgccc gcactatcat tgataattgt gcacatccta tgtatcggga ttatctgcat
    41221 cgctatctgg aaaatgcgcc tggcggacat attcaccacg atcttagcca cgtcttcgac
    41281 ttacaccgta atttaattgc aaccggctcg atgctgggtt aatgactcgt cccgtcacga
    41341 tcttcagcaa tatgctgaag catcgtggcg gtataacgat gatttctgat tgaataaaga
    41401 tattcctgca tatacatcgg cctgtgcggt gcatcaaaat atttcagcgt tgccggattc
    41461 accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat gtccgcgctc cacggcgctg
    41521 attttggcca taaaactgta gggacgataa ataatggttg gattgatccc acaaggtcgc
    41581 atattcgcat caatcattgc ttcgaagttg gcccggttct gaaagcgcat ttgtagccag
    41641 ggcagctcct gaagcagctc ttgctcttga cgctcttcat aacggcgaga aacgagaaag
    41701 cctaatcgca gcggtggtag ttcactgata gtcaggtttt ccagttcctg aactcgtgca
    41761 gatacatgct gtggggagat aataaaatct aactgacggt caaaaagatt atcgataacg
    41821 ccgttttcgc tgaactcaac tggctgggcg gtcacgcctt catacttatc tccgagacta
    41881 atcaactgat cgaaaataat tgtcggataa gtattatcta tgccaatcac aatttctcgt
    41941 gaacgacgcc ctgagttatg gatttcatta tcaatcgcag aaagccgttg ataaaccggg
    42001 aacagttttt aatacagttc ctgtccggct ttattgagac tgatattatt atctttacgg
    42061 gtaaatagcg tatagccaat ttgttcttcc agcgcggcaa tgcttttacc aaatggcgaa
    42121 gccgtcatat ggattttttc tgccgcccgg gcaatattat tggtttgcgc taataaaatg
    42181 aaattgcgca tctttttcga aataaaaatg tccatccccc tcccccgcat ccttgtccgc
    42241 ttaaccgttt cagtcagcct atccttgatg aaaccgcgag caaagatagg tgattacgtc
    42301 atggttttac agaaaattac agaaaaagga ggcaatatcg ggtaaaggca ttagcccgac
    42361 gaatacgtcg ggctacaagt attattgtgc tgcaggtgtt ttagcgggtt gttgatccac
    42421 aggttctaac tggaagacca catcgacctg atcatcaaac tgaatagcgg cctgctcgta
    42481 agtttcctgg gcggacaccg gcgcggcatc ggctttcatc atccgcacca ttgggctggg
    42541 ctgatagttg gaaacatggt agcgcacgct atataccggc cccagtttac gatgaaagcc
    42601 gttcgccagt tcctgcgcct gatgaatcgc gttatcaatc gctgccttac gcgctttgtc
    42661 tttataggca tctggctgcg ccacgcccag cgacacagaa cgaatttcgt taagacccgc
    42721 cttcagcgcg ccatccagca aggaattcag tttgtctaac tgacggagcg tgacttccac
    42781 cgttctcaca gcgcggtagc ctttaaggat acttttacca tcctgataat cataatctgg
    42841 ctgggtgcgt aagttcgctg agctgatatc ttttttcgcg atctgattga gttcaaggaa
    42901 ggaaatgtat tgtgcgacgc gctcatctgc ctgtttcttg gcagtagcgg catccttcgc
    42961 ggccacgtta acttcaatcg caagagtggc aatgtctggc accgcatcca cacttgccgt
    43021 accggaggtg acaatatgcg gtccatccgg caattcgtta gcctgcgctg ccatcccgct
    43081 aatacccatt aatgccgcca gggcgataac tttgaacttc acgttagtct ctccgtgtta
    43141 attgacttgc ccaaaattca ggacctctgg aggcaagctt agcgcctctg ttttattttt
    43201 ccatcagata gcgcctaact gaacaaggct tgtgcatgag caataccgtc tctcgccagc
    43261 tgcaaggcta taaaccacat gacacatccc actaccagat tgataatgcg ctgtgatttt
    43321 gccgtgcgca ggcgcggtgc cagccaggct gcgagaatag ccagaccaaa gaaccacagg
    43381 aaagaggcgc taattgtccc gagcgcaaac caacgttttg gttcgacgtc aagctgcccg
    43441 ccaaggctgc ccagtacaac aaaagtatcc aggtaaacat gcggattcag ccaggtcact
    43501 gccaacatgg tggcgataat tttccatctt ccttgcttca ggacttcggc actggctaac
    43561 tcaatattac tgctcattgc tgttttaaaa gcgccaaaac cataccagag caagaaggct
    43621 acgccgcccc aggtgaccag caccagcaac cacggcgact gcatcaataa cgcgctgcca
    43681 ccaaaaatcc cggcgcaaat caggaccaaa tcgctgatag cacaaagtaa ggcaatcata
    43741 atgtggtact gacgacgaat gccctgattc atcacaaaag cattttgtgg accgagcggt
    43801 aggatcatag ctgcgccaag cgcaagacct tgaaagtaat aagatatcac gtaatttatt
    43861 ccgagctaat tatctgattt gcagactata gcgtgtgagc atcattagag gaaatggata
    43921 tttttaatag catattaata atattaataa taagaaaacg aacagtcagg atgcgtggct
    43981 attttttgga taacaatatc gctcacagga atcgtgctac ggccattacg gcctcgcttt
    44041 ataaaggact cacacaatgc atgcttatga tcttaccctc ttgccccatc ctatccgcga
    44101 aaatatgccg cgccaacaag gttggtgctt tggcctaccg ccaggcataa cgcccgaaca
    44161 gtggccgctg gatccaaata atggtttccc attgaatcat ggatttaccc tgctgttgcc
    44221 ggaagattat cggatttatg ggccagagat tgtcgcgtta tctttctttg ctgttgcgcc
    44281 cgagcaccga tcacagga
//
LOCUS       DAZUGK010000016        42874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZUGK010000016 DAZUGK010000000
VERSION     DAZUGK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.016"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            191..1408
                     /gene="alaA"
                     /locus_tag="ACHY5W_000762"
     CDS             191..1408
                     /gene="alaA"
                     /locus_tag="ACHY5W_000762"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFS4445709.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1492..2091
                     /gene="yfbR"
                     /locus_tag="ACHY5W_000763"
     CDS             1492..2091
                     /gene="yfbR"
                     /locus_tag="ACHY5W_000763"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFS4445710.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2150..3982)
                     /locus_tag="ACHY5W_000764"
     CDS             complement(2150..3982)
                     /locus_tag="ACHY5W_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS4445711.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSMREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4069..4719)
                     /gene="hxpA"
                     /locus_tag="ACHY5W_000765"
     CDS             complement(4069..4719)
                     /gene="hxpA"
                     /locus_tag="ACHY5W_000765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFS4445712.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4730..5224)
                     /locus_tag="ACHY5W_000766"
     CDS             complement(4730..5224)
                     /locus_tag="ACHY5W_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4445713.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5306..5761)
                     /gene="yfbV"
                     /locus_tag="ACHY5W_000767"
     CDS             complement(5306..5761)
                     /gene="yfbV"
                     /locus_tag="ACHY5W_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFS4445714.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6099..7301
                     /gene="ackA"
                     /locus_tag="ACHY5W_000768"
     CDS             6099..7301
                     /gene="ackA"
                     /locus_tag="ACHY5W_000768"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFS4445715.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7376..9520
                     /gene="pta"
                     /locus_tag="ACHY5W_000769"
     CDS             7376..9520
                     /gene="pta"
                     /locus_tag="ACHY5W_000769"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4445716.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9710..11230
                     /gene="yfcC"
                     /locus_tag="ACHY5W_000770"
     CDS             9710..11230
                     /gene="yfcC"
                     /locus_tag="ACHY5W_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4445717.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11263..11805)
                     /gene="yfcD"
                     /locus_tag="ACHY5W_000771"
     CDS             complement(11263..11805)
                     /gene="yfcD"
                     /locus_tag="ACHY5W_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFS4445718.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11863..12414)
                     /gene="yfcE"
                     /locus_tag="ACHY5W_000772"
     CDS             complement(11863..12414)
                     /gene="yfcE"
                     /locus_tag="ACHY5W_000772"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFS4445719.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12470..13114)
                     /gene="yfcF"
                     /locus_tag="ACHY5W_000773"
     CDS             complement(12470..13114)
                     /gene="yfcF"
                     /locus_tag="ACHY5W_000773"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFS4445720.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFVGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13250..13897
                     /gene="yfcG"
                     /locus_tag="ACHY5W_000774"
     CDS             13250..13897
                     /gene="yfcG"
                     /locus_tag="ACHY5W_000774"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFS4445721.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13954..14316
                     /gene="folX"
                     /locus_tag="ACHY5W_000775"
     CDS             13954..14316
                     /gene="folX"
                     /locus_tag="ACHY5W_000775"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFS4445722.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14337..15230
                     /locus_tag="ACHY5W_000776"
     CDS             14337..15230
                     /locus_tag="ACHY5W_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFS4445723.1"
                     /translation="MNIVITGGTGLIGRHLIPRLQELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15278..16168)
                     /gene="rpnB"
                     /locus_tag="ACHY5W_000777"
     CDS             complement(15278..16168)
                     /gene="rpnB"
                     /locus_tag="ACHY5W_000777"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFS4445724.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16365..17138)
                     /gene="hisP"
                     /locus_tag="ACHY5W_000778"
     CDS             complement(16365..17138)
                     /gene="hisP"
                     /locus_tag="ACHY5W_000778"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFS4445725.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKHEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17146..17862)
                     /locus_tag="ACHY5W_000779"
     CDS             complement(17146..17862)
                     /locus_tag="ACHY5W_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4445726.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17859..18545)
                     /gene="hisQ"
                     /locus_tag="ACHY5W_000780"
     CDS             complement(17859..18545)
                     /gene="hisQ"
                     /locus_tag="ACHY5W_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFS4445727.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18635..19417)
                     /gene="hisJ"
                     /locus_tag="ACHY5W_000781"
     CDS             complement(18635..19417)
                     /gene="hisJ"
                     /locus_tag="ACHY5W_000781"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFS4445728.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19638..20420)
                     /gene="argT"
                     /locus_tag="ACHY5W_000782"
     CDS             complement(19638..20420)
                     /gene="argT"
                     /locus_tag="ACHY5W_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFS4445729.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAFANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20685..21254)
                     /gene="ubiX"
                     /locus_tag="ACHY5W_000783"
     CDS             complement(20685..21254)
                     /gene="ubiX"
                     /locus_tag="ACHY5W_000783"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFS4445730.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21349..22866)
                     /gene="purF"
                     /locus_tag="ACHY5W_000784"
     CDS             complement(21349..22866)
                     /gene="purF"
                     /locus_tag="ACHY5W_000784"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4445731.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22903..23391)
                     /gene="cvpA"
                     /locus_tag="ACHY5W_000785"
     CDS             complement(22903..23391)
                     /gene="cvpA"
                     /locus_tag="ACHY5W_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFS4445732.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23832..24494)
                     /gene="dedD"
                     /locus_tag="ACHY5W_000786"
     CDS             complement(23832..24494)
                     /gene="dedD"
                     /locus_tag="ACHY5W_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFS4445733.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24484..25752)
                     /gene="folC"
                     /locus_tag="ACHY5W_000787"
     CDS             complement(24484..25752)
                     /gene="folC"
                     /locus_tag="ACHY5W_000787"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFS4445734.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGKELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGCMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25822..26736)
                     /gene="accD"
                     /locus_tag="ACHY5W_000788"
     CDS             complement(25822..26736)
                     /gene="accD"
                     /locus_tag="ACHY5W_000788"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4445735.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26892..27551)
                     /locus_tag="ACHY5W_000789"
     CDS             complement(26892..27551)
                     /locus_tag="ACHY5W_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS4445736.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27634..28446)
                     /gene="truA"
                     /locus_tag="ACHY5W_000790"
     CDS             complement(27634..28446)
                     /gene="truA"
                     /locus_tag="ACHY5W_000790"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4445737.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28446..29459)
                     /locus_tag="ACHY5W_000791"
     CDS             complement(28446..29459)
                     /locus_tag="ACHY5W_000791"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4445738.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTCQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVEALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29525..30661)
                     /gene="pdxB"
                     /locus_tag="ACHY5W_000792"
     CDS             complement(29525..30661)
                     /gene="pdxB"
                     /locus_tag="ACHY5W_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFS4445739.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30760..31755
                     /gene="flk"
                     /locus_tag="ACHY5W_000793"
     CDS             30760..31755
                     /gene="flk"
                     /locus_tag="ACHY5W_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFS4445740.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31752..32930)
                     /locus_tag="ACHY5W_000794"
     CDS             complement(31752..32930)
                     /locus_tag="ACHY5W_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4445741.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGISVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWRLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGSGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33205..34425)
                     /gene="fabB"
                     /locus_tag="ACHY5W_000795"
     CDS             complement(33205..34425)
                     /gene="fabB"
                     /locus_tag="ACHY5W_000795"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFS4445742.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34584..36590
                     /gene="mnmC"
                     /locus_tag="ACHY5W_000796"
     CDS             34584..36590
                     /gene="mnmC"
                     /locus_tag="ACHY5W_000796"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFS4445743.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHHLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMKKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36711..36989)
                     /locus_tag="ACHY5W_000797"
     CDS             complement(36711..36989)
                     /locus_tag="ACHY5W_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFS4445744.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37023..37571)
                     /gene="epmC"
                     /locus_tag="ACHY5W_000798"
     CDS             complement(37023..37571)
                     /gene="epmC"
                     /locus_tag="ACHY5W_000798"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFS4445745.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADVEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37571..38380)
                     /locus_tag="ACHY5W_000799"
     CDS             complement(37571..38380)
                     /locus_tag="ACHY5W_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4445746.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38380..39204)
                     /gene="mepA"
                     /locus_tag="ACHY5W_000800"
     CDS             complement(38380..39204)
                     /gene="mepA"
                     /locus_tag="ACHY5W_000800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFS4445747.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39208..40293)
                     /gene="aroC"
                     /locus_tag="ACHY5W_000801"
     CDS             complement(39208..40293)
                     /gene="aroC"
                     /locus_tag="ACHY5W_000801"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4445748.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAETFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40328..41260)
                     /gene="prmB"
                     /locus_tag="ACHY5W_000802"
     CDS             complement(40328..41260)
                     /gene="prmB"
                     /locus_tag="ACHY5W_000802"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4445749.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41426..41977
                     /gene="smrB"
                     /locus_tag="ACHY5W_000803"
     CDS             41426..41977
                     /gene="smrB"
                     /locus_tag="ACHY5W_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFS4445750.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42091..>42874)
                     /locus_tag="ACHY5W_000804"
     CDS             complement(42091..>42874)
                     /locus_tag="ACHY5W_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFS4445751.1"
                     /translation="QADVTFNNVQTYGASYGSGLTTSIVYKIDVLTPEGAYYGTYSQG
                     GIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCPGMTSGWVCHRLPVNVHVTG
                     TTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPTEPVASYDISWDENTVVHNK
                     SLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYCRLVSQLMTFSVQGCDNNKV
                     NVTANRHPVTDKQLHDMQVRVDTSERQSFDATCRFQYILNEL"
BASE COUNT        10562 a        11622 c        10738 g         9952 t
ORIGIN      
        1 taacattgca tgtaaagcat atacacctca ttattttgtc attattaagt tattaacagc
       61 acaatcgagc cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg
      121 ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag
      181 gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat
      241 ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa
      301 actgaacatc ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga
      361 cgtgatacgc aacctgccta cagctcaagg gtattgcgat tccaaaggtc tttactccgc
      421 gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga
      481 tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa
      541 cagcggggac gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc
      601 gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga
      661 cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc
      721 aaataaccca accggcgcgg tatattccaa agagctttta atggagattg tggagattgc
      781 acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga
      841 cgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaatgg
      901 actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc
      961 gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg
     1021 tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag
     1081 tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaattgat
     1141 caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtaca tgttcccgaa
     1201 aatcgacgcc aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt
     1261 gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt
     1321 ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg
     1381 tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc
     1441 agtgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag
     1501 agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc gctcatgcgc
     1561 aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg
     1621 ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta
     1681 ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa
     1741 tacttcaatt cgcaaatcgc tcaggaatac aaggctattg aaaaaatcgc tcagcaaaaa
     1801 ctggtcgata tggttccgga agagctgcag gatatctttg cgccgttaat tgacgagcat
     1861 gcatatagcg atgaagaaaa atcgttggtg aaacaggcag atgcactgtg tgcatatctg
     1921 aaatgtctgg aagaactcgc ggcgggaaat aatgaattct tgctggcaaa aacgcgactg
     1981 gaagcgacgc ttgaagcgcg ccgcagccag gagatggact acttcatgga agtatttgtt
     2041 cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta agcagctgga
     2101 gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa
     2161 acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca
     2221 acttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccagggtg ttaaccggtg
     2281 aagaaaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat
     2341 aaggcgacac acccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg
     2401 tcgcggtatt agagataaat agcccaataa ccgccgacaa gacaaacaaa caacccagca
     2461 tcatatgtgg cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac
     2521 ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg
     2581 gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca
     2641 ggcaggcgat gatagcggca acaggattag gaatttcatc ggtcagcatt aacgccacca
     2701 ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca
     2761 cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca
     2821 gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg
     2881 aaccttccag cgccacacca ttgcgcttta gccccaccac attcagtccg tagcgggtac
     2941 gaaaaccaat ttcgcgcatc gatttaccaa tcagttctga ctcaggaatt aatgaaatct
     3001 ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct
     3061 ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa
     3121 gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa
     3181 aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat
     3241 cgtccagccg ttgaccaatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc
     3301 cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct
     3361 gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca
     3421 gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt
     3481 cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc
     3541 cgctaatcag cccggcaaaa cttaacggca tcatcagacg tgacggcgac gtttgcatac
     3601 gcatggcaac gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa
     3661 acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc
     3721 ccgcaacttt gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat
     3781 cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata
     3841 cttctgggac tgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg
     3901 catccatacg cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa
     3961 cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct
     4021 gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat
     4081 aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc
     4141 gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc
     4201 cgccaggcca gaaagcacgc cagcgggagc atcttccacc accacacact cttgcggcgc
     4261 aagccccagc agctgcgcgc ctagcagata cgcatcaggt tctggttttc cgcgcttcac
     4321 tcgctcagcg gttacaaaca cctctggcgc gggaagcccc gctattttat ggcgcgctcg
     4381 cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg
     4441 attgagtaag gcgattgccc ccggaagcgc ggtaatacct tcggtttccg tggcctcgat
     4501 ttgctccaga cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg
     4561 gcgcagagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa
     4621 cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag
     4681 cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga
     4741 taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat
     4801 taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaaccg tgagttccgg
     4861 cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac
     4921 gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct
     4981 ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata atgtcgatga
     5041 tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccagttca cgcatctgta
     5101 atccataacc acgctcaatg attgtttgca ggcgacggta acgttcagca tttgcaggat
     5161 cgagcatagt catcatcttg tactggttag acaaaataag acgttgcgcg ttggtcattt
     5221 ccattgttga ctcctgtatc actctattac ggtgaaaaaa agaaggctga gtatgccttc
     5281 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
     5341 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
     5401 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttgaggatag
     5461 cagggggtaa tggcgtgaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
     5521 ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc
     5581 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
     5641 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt
     5701 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
     5761 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
     5821 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
     5881 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
     5941 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
     6001 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
     6061 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
     6121 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
     6181 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
     6241 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
     6301 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
     6361 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
     6421 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
     6481 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
     6541 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
     6601 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
     6661 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
     6721 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
     6781 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
     6841 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
     6901 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
     6961 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
     7021 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
     7081 ttgtattcac cggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
     7141 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
     7201 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
     7261 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
     7321 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
     7381 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
     7441 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
     7501 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
     7561 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
     7621 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
     7681 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
     7741 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
     7801 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc
     7861 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
     7921 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
     7981 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
     8041 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
     8101 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
     8161 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
     8221 ccgtcctgac gtgctggttg ccgcttgcct ggctgccatg aacggcgtag aaatcggtgc
     8281 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
     8341 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cttctctgag
     8401 cctgcagagc ttcaacctgg aagttccagt tgacgatcac gagcgtatcg agaaagttca
     8461 ggaatacgtt gctaactaca tcaacgctga ctggatcgat tctctgactg ccacttctga
     8521 gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac ttgcgcgcaa
     8581 agcgggcaaa cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
     8641 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
     8701 ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggc attgaaatcg ttgatccaga
     8761 agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac
     8821 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga
     8881 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
     8941 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
     9001 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
     9061 cgcagaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcag ccttcggtat
     9121 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
     9181 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
     9241 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcta aagcgccgaa
     9301 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
     9361 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
     9421 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
     9481 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
     9541 ttgcgctgcg gatatctgaa ccggaaatag tcactatttc cggtttttta ttctcttaat
     9601 ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc
     9661 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
     9721 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
     9781 tgttgctatt ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
     9841 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
     9901 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
     9961 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc
    10021 gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    10081 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    10141 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    10201 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    10261 gctgggctat gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt
    10321 tgccagttcg tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc
    10381 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct
    10441 gatctttacc atggtctacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    10501 tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt
    10561 tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt
    10621 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    10681 gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
    10741 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    10801 agggattttg ctgctggttg gtaatggtga agcaggtgat gccagcgtgt taaatactat
    10861 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    10921 gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt
    10981 aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt
    11041 gctggcgttc cagtttggtg atggcttcag tcacatcatt tacccaacct cagcttcgtt
    11101 aatggcaacg ctcggtgtgt gcagggtgga cttccgtaac tggctgaagg tgggcgcgac
    11161 cctacttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    11221 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    11281 ctctacggct tcatttttgg cattgcgctt catccacaac gctagcgctt tcagcgagtc
    11341 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact
    11401 gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca atgcgcccca
    11461 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    11521 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    11581 gactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    11641 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    11701 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
    11761 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    11821 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    11881 ctgcgcaatg atactttgat cattgagtgc gattacactg agtacgtcat tatcgagcat
    11941 gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    12001 aatctccccc cgctgttctg ccactggcag atgggtatgg ccgtacacca gcacatcgtt
    12061 ctggtttaaa gcaggcagat tttctgggcc aaaaagatga ccatgcgtca aaaacagacg
    12121 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg
    12181 atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag
    12241 ccgttcagcg actttggctg gcgcataacc ctccggtaaa gcattacgcg ggccgtgatt
    12301 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac
    12361 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    12421 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    12481 gcttcgctga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
    12541 aatccaccag gcattctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    12601 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtactaaca
    12661 aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    12721 cttttttcgc ccccacaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    12781 cgctgcgcaa ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    12841 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    12901 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    12961 cttgccacgt cggttgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    13021 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaacagtggg
    13081 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    13141 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc
    13201 gcgctttcta tacttgtggt gttcagatga ttagacaacc ggagctgcaa tgatcgatct
    13261 ctatttcgcc ccgacaccca atggccacaa aattacgctg tttctcgaag aagcagggct
    13321 ggattatcgc ttgataaagg tagacctggg gaaaggcggt cagtttcgcc cggaattttt
    13381 gcgcatttcg cctaacaaca aaattccggc aattgttgat cattctccag ccgatggcgg
    13441 cgaaccgcta agcctctttg aatctggtgc cattttgttg tatctggctg agaaaacggg
    13501 gctctttttg agtcatgaaa cgcgtgaacg cgccgccaca ttacagtggt tattctggca
    13561 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    13621 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtctgt accatgtact
    13681 gaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    13741 tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg caatgtatcc
    13801 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    13861 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    13921 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    13981 ataaagaacc ttcgtttacg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    14041 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg caccagcgaa
    14101 gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag
    14161 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    14221 catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc
    14281 gattcggtat ccatgacctt aagctggcag cgttaaccgc catatcggga ggctgcatga
    14341 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattcca cgtttgcagg
    14401 agctgggcca tcaaatcacg gtaatgacgc gtaacccgca gaaagccagt tccgttctcg
    14461 gccctcgggt gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg
    14521 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac gagcaaaaag
    14581 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca
    14641 gcgacacgcc accgtcggta ctcatttccg gctcagcaac gggctattat ggcgacttag
    14701 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttacccat aaactctgcg
    14761 cccgctggga agaaattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    14821 gtaccggtgt agtgctggca ccggatggcg gtattctcgg taaaatgctg ccgccgtttc
    14881 gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg attcatatcg
    14941 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    15001 tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    15061 atcgcccggc cattttgcgc gtccccgcga ccgccattcg actgttaatg ggcgaatctt
    15121 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    15181 cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    15241 agcaaacatc cgccagttaa ctcccggtgt tacaggatta gtggctttgc gcgataagat
    15301 cgtctggtga aagtcgggtc agcgtcagga tgacttcgtg gtcaaatcct ttttccagca
    15361 tctcctgagc aatacgcaga gcttcttcgt gtttgccctg cattgcgcct tcttcacgta
    15421 atctgtcagc aatggtcatc agtttctcct tttcctgtgg tgcgcgttct gctatctcac
    15481 caataaatgc acggaaacgc tgggcatctc ccgtttgtaa tacataatta aacagggctt
    15541 ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc
    15601 ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcgat
    15661 gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaaa gccgatgaat
    15721 atattttacg ggcaatcgca ggctcggcaa attcatcaag ccagcagagt gaataaggat
    15781 aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc
    15841 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
    15901 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
    15961 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatct ataaaactgt
    16021 ttggttccag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa
    16081 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
    16141 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
    16201 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
    16261 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
    16321 tccccttgaa aatactccgg cagcgttagc caccggagag gcaattattt cagcgaacct
    16381 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgcct
    16441 tcttcctcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg acgagcaaag
    16501 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataatg
    16561 cgcaacactt cgcctacaag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
    16621 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
    16681 agatgcaccg gatatttccc ctgtgcacgt tcgtctatcc cgacttttgc cagatacttc
    16741 accgcccgct cgcgcgcttc atgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    16801 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
    16861 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
    16921 cgcaccagat tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg
    16981 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
    17041 acatctccgg cattcgcttg cagtgacacc cctttcagca cttcgtgttc gccatagcgt
    17101 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    17161 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
    17221 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
    17281 ggcgttaata tcgcgggcta ttttcagcag atccggcacc gtggcagtaa atgccaacgc
    17341 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    17401 agaaggcaaa ataatgcagc gatacatttt aaaagtcgag aagccatagg ctctggcggc
    17461 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
    17521 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga aaaaggcgtt
    17581 aagaaactcg gtccccttaa caatctccag cgtatacatg ccggaataga acaccagcaa
    17641 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    17701 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccagaactc cgcctatcac
    17761 taccgacaaa ataagcagcc acagagtgat cgccacgccg gtaaagcgat aaccgtcggt
    17821 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
    17881 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc
    17941 aggtaaatca cgccacagac tatggcgaaa tagaacggct cccaggtact tttgcctgcc
    18001 agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    18061 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    18121 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
    18181 cctttcggca ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata
    18241 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
    18301 gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaacact
    18361 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
    18421 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
    18481 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
    18541 aacatgcagc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    18601 ccgaagggag cgggctttct ttcaccgatg gcgattagcc accataaaca tcaaaatcga
    18661 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
    18721 tcagtgcctc gcgcagttcg ttatcttctt tacgtaggcc catgccggtc cctacgccaa
    18781 acagtttttc atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt
    18841 taaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    18901 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
    18961 cccagtgttc gttaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    19021 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat
    19081 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
    19141 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
    19201 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
    19261 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
    19321 cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg
    19381 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    19441 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    19501 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc
    19561 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    19621 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
    19681 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaagc agccgtcagt
    19741 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
    19801 actgatgagc cagcaaaggc gaagtcttta ccagcaggtt gcttgaggaa tccttcgctg
    19861 gcagcaactt catcctgcag tgcagcatcc agacgtcctg cagccagatc ggaatagacc
    19921 aaatcctggt tggcataggc caccacatcc acacctttgc tacgccaggt ctcgttagcg
    19981 aaagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    20041 gttggctgaa tcggcgaacc tttggcggca atcaaacgag aatctgcggc gtacaacttg
    20101 tcggagaaag caatctcctg ctgacgttta tcggtaatgg agagcgacga aataatggcg
    20161 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
    20221 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
    20281 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
    20341 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
    20401 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggcttttta tgttgtgtat
    20461 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    20521 acattcaacg ttaaatgtgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    20581 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    20641 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    20701 cgggcaaaga gatcttcagg aagggttatc gcaaactggt caagaacacg attaaccgtc
    20761 tgatttatca catcatcaag ggattgtggg cgatgataaa acgccggaac gggaggcata
    20821 atcaccgcac cgatttctgc tgcctgcgtc attaaacgca gatggcctaa gtgcaatggt
    20881 gtttcacgca cgcagagcac caacgggcga cgctctttca gcacaacatc tgccgcacgg
    20941 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    21001 aaaatcacca tccccaacgt ctggaaagaa ccggaagaga tgctggcggc aatatcgcgc
    21061 gcatcgtgcg tgacatcggc taacgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    21121 gataaggtct ggcgcgctgc ctggctcatt accagatgcg tttcgatatc tgtgacatcg
    21181 cgcagaacct gtaataaacg cacgccataa atcgcgccgc tggcaccgct gatgcccaca
    21241 ataagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg caggatttcg
    21301 gcgggagttg caagtcaggg tgccagatcg gcaccctgag cgaaggcatc atccttcgtt
    21361 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt cgtcattacg
    21421 taaagtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    21481 gaacaccgag cattcaaact gctggatatc cgggttttca gcgcgaacgg cgtcgatcag
    21541 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatctac
    21601 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    21661 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    21721 catctcgata atctgctcag aagtggtgcc acggacgata gagtcgtcga ccagcaggac
    21781 gtttttatcg cggaactcgg cgcggttggc gttcagttta cggcgcacgg acttacgacg
    21841 cagctgctga cccggcatga tgaaggtacg gccaacatag cggtttttaa cgaagccctg
    21901 acggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttccgg
    21961 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    22021 cttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgatga acgagtccgg
    22081 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    22141 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    22201 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    22261 gcggttctcg tcaatatcac gttttcccag aaccagcgga cgaataccgt ttggatcgcg
    22321 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    22381 gcggtttgta gcagcgatgg cggcgaaaat gttgtcggct tccagcgggt agtggcggaa
    22441 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    22501 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    22561 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    22621 gctggagctg ccagccgtgg ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    22681 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    22741 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    22801 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    22861 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    22921 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga actgcgggat
    22981 cagctgtgat ttgctccagt cttcgctttt cgacaccccg gtaaaggagt cgagaaagaa
    23041 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    23101 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    23161 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    23221 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    23281 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgcgga tcaggctaac
    23341 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtcct
    23401 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    23461 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    23521 cgacttttta tgcctttgcg gcatcgttca atgcgtgtcg gatgcggcgt aaacgcctta
    23581 tccgacctac agttctaacc ctgcgtaggc ctgataagac gcgccagcgt cgcatcaggc
    23641 aacggctgtc ggatgcggcg taaacgcctt atccgaccta cagttctaac cctgcgtagg
    23701 cctgataaga cgcgccagcg tcgcatcagg caacggctgt cggatgcggc gtaaacgcct
    23761 tatccgacct acggctctac ccctgcgtag gcctgataag acgcgccagc gtcgcatcag
    23821 gcaagaccgt attaattcgg tgtataaccc attaccacgc cgcttaagcc agaaagttgc
    23881 ttcaactcac ccagcgaacc tttcagctta tctttcgagg catctggccc aaccagaata
    23941 cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg
    24001 cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcgcc cagttgcaca
    24061 acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc
    24121 ggcgcaggtg gtgcttcaac cttcggctta ggcggctcca ccggtttcgg cttcggtggg
    24181 acgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg
    24241 agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc
    24301 gtcggcaacg cctgggtggc ggctggcatc atatcaggtt catcgcgatc gcccgctttt
    24361 ggcaccagcg ggatagccgc gaactcatcc tgataatgtt ttttctgccc gtccagcagc
    24421 cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga
    24481 aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg
    24541 gaacgaacca cacaccagca cggtatcttc cgctttagcg tccgccattg cggcatccca
    24601 tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc
    24661 cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa
    24721 ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg
    24781 cccgtttttc ggtagcgctt tcatacaccc ggtgagatat tccgccgcat gtggattatg
    24841 cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat
    24901 tgcgctggca atcccgtcgc gaatggcatt ttcactgacc tccagcccgc tggcacgcag
    24961 tgccgccagc gccgttgcgg cattcggttg cgggacaagc ggcaacggca ggttttccag
    25021 cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac
    25081 gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat
    25141 ttccggctca ccgacaattg ccggtttttc gctgcggaag atgcctgctt tctcgcgacc
    25201 aatactttcg cgatctggcc ccagccagtc ggtatgatcc agcgcaatac tggttactac
    25261 cgcgacatcg gcgtcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag
    25321 aatcaccacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg taccgtactc
    25381 gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg
    25441 ggccgattcc ggcaattcct tgccctgcac acgtacgcgc tcggtataac gcaccagatg
    25501 cggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg
    25561 gcaggtggtg cctttgccat tcgtacccgc aacggtaaat acaaatggcg ctggtttcag
    25621 gacgccaaga cgcgccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact
    25681 gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg
    25741 tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag
    25801 tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac
    25861 tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat
    25921 gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc
    25981 gatcaggaat tcactgcgct ggaatcctgg cggcagtttt tcgcgaacgg tctgttcgat
    26041 aacacgcgga ccggcaaagc cgattaacgc tttcggttca gcgatgttga gatcgcccag
    26101 catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg
    26161 caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga
    26221 catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt
    26281 atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga
    26341 accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc
    26401 tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc
    26461 tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct cgctacccag
    26521 ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat
    26581 gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac
    26641 ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg
    26701 agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg
    26761 gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat
    26821 gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc
    26881 ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg
    26941 acgcccggca aaatagaaac cacaataatc ccgacgatca gcagcttaag gttatcctga
    27001 accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt
    27061 gcgccgatca cgttataagc ggcgaaatga cggtacgaca tgtggcccat tcccgcaaca
    27121 aacggggcga acgttctgac gatcggcaca aaacgggcga gaataatcgt tttgccgcca
    27181 tgtttctcat aaaactgatg ggttttgtcg agataactgc gacggaaaat tttcgaattg
    27241 ggattactga ataacttttc accgaacaac cgcccgatag tgtaattgac cgcgtccccc
    27301 acaatcgcgg cgatcagcat cagcaccacc atcatatgga cgttgagatc gttggtttcc
    27361 agtgatgcca acgctccggc aacaaacagc aatgaatcac ccggtaaaaa cggcgttacc
    27421 accagaccgg tttcacagaa caaaattaag aacaaaatgg cataaaccca gacgccgtac
    27481 tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa
    27541 atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg
    27601 ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat
    27661 tggcggtttt ggaagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc
    27721 cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca
    27781 gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat
    27841 atgatgtaca aaggcattcg ctttgatatc aaccaccaca taaggaccgt gacgcgtgac
    27901 attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga
    27961 ggtgaaatca ttctcgccca gcaagcactg cgcagcccga tgcatccgtt cagcgtccag
    28021 cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta
    28081 gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac
    28141 agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaacgtcca
    28201 cgcagcgtct ttgcgctgcg cggtagtttc gaaatgcaca acctgcccgg taccgtgtac
    28261 ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag
    28321 cgccttttcc agcttctcct gcacactgcg aacttcattc tgccgttgcc agccgtaata
    28381 cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcggtt gttgctggtc
    28441 ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg
    28501 ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag
    28561 tcattacgca cgcagccaac agaaagatgc ggcgtaccag aagcatcacc tacctgagtt
    28621 gggaattcgt tctcttcaga gagcacaata tcttcgcctt gaacaaacgc atcacgcgct
    28681 tcttctgctg ccagtggacg cagagcttca aagttgacca tctgggcatg accgtagaat
    28741 accggtgcct ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt
    28801 acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg cagtaacggc
    28861 agcatgttga acgccagctg acgcccgaag aaatcttctt cgtcaatcgg aatgccgttg
    28921 agcaatttcg cactctgccc cgctaacgcc tcgaccgctt ttttgccctg ggcggaggct
    28981 gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt
    29041 gccgccagca gctggcaggt cagactgtct ggtacggcga tgacattccg gttgcggtaa
    29101 tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca
    29161 aacaatccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggct
    29221 gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca
    29281 gcggcatcct gcacggtgat tgtcttacca ccaaagcgca gttgctcgcc tgcgctttcg
    29341 ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc
    29401 agggcttcgc ccacagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg
    29461 gtggtttact ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa
    29521 gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg
    29581 cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt
    29641 tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc
    29701 ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcgggccat
    29761 gcagcgtaat gcgaccaaac tctggcgcgg gcagtaatgt atccagcgca acgtgctgtt
    29821 catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt gctttacctt
    29881 ccagggtata gcctgcaata tgcggcgtgc cgatatccac ttttgtcagc aactccacgt
    29941 taagttccgg ctcgccttcc cagacatcca gcaccacgct taacttctgg ccttcattca
    30001 ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg
    30061 ctccgggctt caggctacgg atcagttttt catccgccag atgtagcgtt ttgtacggac
    30121 catctttaaa gagtggcgta tggaaagtca gaatatccgc gcgctggact aactcatcca
    30181 gcgagcggaa atcaccctca tccccacggt cggcgcgagg cggatcgcaa agtaaggttt
    30241 taatccctaa cgcttccagt cgtgcctgta aacgccgacc aacgttacca acgcccacga
    30301 tccccacggt acgctcgtgc agtgaaaatc catcgcgttc ggcaagcatc agcagggagg
    30361 agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc
    30421 ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca gtgccaacaa
    30481 atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca
    30541 gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcgatcacct
    30601 cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca
    30661 cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata atctcgcggc
    30721 caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc
    30781 cctcctcctg ggcaaccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag
    30841 cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg
    30901 acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga
    30961 gattcgccgc tgctttcacg tcactttcct gccgctgagc ataatctggc gcaacgtctg
    31021 ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta
    31081 cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg
    31141 ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg
    31201 aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg atcgtcctct attaccggcg
    31261 gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg cggcaacggg ggaacccagc
    31321 aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca
    31381 gcgaaactgt ttaaccatct ggtgacctgg ctgcaggcgc gtcagacgct aagccagcaa
    31441 aatacgccga cgctggaatc actacaaatg acgctaaaac aacctttaga tgccagtgaa
    31501 ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt
    31561 tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt
    31621 gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa
    31681 aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc
    31741 tggctggttc gttaaccccg acgaaatgac agtatcgtga caataattcc cagcaccgca
    31801 gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc
    31861 agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg
    31921 ccgcgaactt gtgagggaac acgtttaacc acctccacgc ccagcgcagg aaagataagc
    31981 gaacatccgg ctccggttaa cgccgcgccc gctaatgcaa cccacgcacc tggggcttgc
    32041 cagagcagca acaagcccac cgtttctaca agcagagaga cgatcgccac tttcacgccg
    32101 ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac
    32161 gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa
    32221 gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc ctgaccctgg tttccagata
    32281 agcccgacaa cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact
    32341 gtgccgttac aggcccacgc cagtaagggt aatgccattg tggtgagcgc cagtgcggca
    32401 aaaccgtaat ggctatgaat caacaggcca agcggagcac caacagcgag ggcaccgtaa
    32461 atcgccattc cgttccatga cattactttg ccagagtgtt ttggccctac gatgcctaat
    32521 ctccaggtca gagcgcctgt cagtaactgg ctttcaccaa agccaagaat caaacgcccg
    32581 acgaccaaca gggcaaattt gaacggtgcg gagacgggca aaatcgccgc cagcagcaac
    32641 gcgccccccg ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcgccatat
    32701 tgatcggcca gtcgcccggc gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg
    32761 acggaaatgc cgaccatggt attgccatag cccagttcat gatgaacaaa caacgggata
    32821 accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag
    32881 agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc
    32941 agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacggt
    33001 gcgttggcag gaattaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt
    33061 gcctggaaca ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcggg ttccctttgg
    33121 cgttagtgcc gaatgccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc
    33181 cgacctactc cggcctactg cgaattaatc tttcagcttg cgcattacca gcgtggcgtt
    33241 ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat cggtcgtttc
    33301 ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga tgctcggggc
    33361 gataaagccg tgttccagca tcagcagaga gtagatagct tcctgtacgc cagcagcgcc
    33421 cagagagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat cgccgaacac
    33481 ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtac cgtgggagtt
    33541 caggtaatcg attggggtat caacgccatg catcgccatc ttcatgcagc gtactgcgcc
    33601 ttcgccagac ggagcaacca tgtctgcgcc atcagacgtt gcgccgtagc caacgatttc
    33661 agcatagatg tgagcaccac gcgccagcgc gtgttccagc tcttcaacca ccaccatacc
    33721 gccgccgcca gcgataacga aaccgtcacg gtgagcatcg taagtacggg aggctttttc
    33781 cggggtgtcg ttgtatttag tagacagcgc acccattgcg tcgaattcgc aagccatttc
    33841 ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca gttggatctg
    33901 ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga tggagtagtt
    33961 aacgccatga attttaaacg gggtggcgag gcaggcagaa acgccggatg ccatcgcttt
    34021 ggtgaccaca tacgggccaa ccgctttcag gccacgcggg ccgcgcattg cgtcagcgcc
    34081 gaacacctgg aaacgcgggg aaccgccgcc agaacctgca atcaggccaa cgcgcggatt
    34141 attctggtaa gcttccggag aaaggcccgc atctgcgatt gcctgctcca tagaaaggaa
    34201 tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga ggccagtggt
    34261 atccagtttt acgttgcccc agacgtggct acgcatgccg gaatccttca gctcctgaga
    34321 gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct ggttattacc
    34381 gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat acctctgtaa
    34441 gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc
    34501 gatcagccaa ttaatagaga aatttgcgca gccttacaca catcgctaag atcgagccac
    34561 cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc aacctcgaat
    34621 ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt tccaacgata
    34681 acgggctgga agagacgcgt tatgtttttc tcggaggtaa ccaattagag gcacgctttc
    34741 ctgagcatcc acatcatctg tttgtggtag cagagagcgg cttcggcacc ggattaaact
    34801 tcctgacgct atggcaggca tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac
    34861 aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat ttagccttag
    34921 cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg cagtggccaa
    34981 tgcccttgcc cggttgccat cgtttattac tcgatgaagg ccgcgtgacg ctggatttat
    35041 ggtttggcga tattaatgaa ctgaccagcc aactggacga ttcgctaaat caaaaagtag
    35101 atgcctggtt tctggacggc tttgcgccag cgaaaaaccc ggatatgtgg acgcaaaatc
    35161 tgtttaacgc catggcaaga ttggcgcgtc cgggcggcac gctggcgaca tttacgtctg
    35221 ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgaaaaaa cgtaagggct
    35281 ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca aacattacca ctcccctgct
    35341 ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt atcggcggtg
    35401 gtattgccag cgcattgttg tcgctggcgc tattgcgtcg cggctggcag gtaacgcttt
    35461 attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg gcgttgtatc
    35521 cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatgcg tttacttttg
    35581 ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca tgactggtgc ggcgtcacgc
    35641 agttaggctg ggatgaaaaa agccagcata aaatcgcaca gatgttgtca atggatttac
    35701 ccgcagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg ggcgttgcga
    35761 caaattgcag cggcattact tatccgcaag gtggttggct gtgcccagca gaactgaccc
    35821 gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa tatcagttac
    35881 aggatttatc ccgtaaggat gactgttggt tgttgaattt tgcaggagat cagcaagcaa
    35941 cacacagcgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc caaacatcat
    36001 ctctgccagt atattcggtt gccggtcagg tcagccatat tccgacgaca ccggaactgg
    36061 caaaactgaa gcaggtgctg tgctatgacg gttatctcac gccacaaaat ccggcgaatc
    36121 agcatcattg tattggtgcc agttatcatc gcggcagcga agatacggcg tacagtgatg
    36181 aagatcagca gcagaatcgc cagcggttga ttgattgttt cccgcaggca cagtgggcaa
    36241 aagaggttga tgtcagtgag aaagaggcgc gctgcggtgt gcgttgtgcc actcgcgatc
    36301 atctgccaat ggtaggcaac gtgcctgatt atgaggcaac actcgtggaa tatgcgtcgc
    36361 tggcggagca gaaagataag gcggtaagcg cgccggttta tgacgatctc tttatgcttg
    36421 ctgctttagg ttcgcgtggt ttgtgttctg ccccactgtg cgcagagatt ctggcggcgc
    36481 agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta aatccgaatc
    36541 gattatgggt gcggaaattg ttgaagggta aagcagttaa ggctgggtaa ccagctttgg
    36601 cattgtttgt cggatgcggc gtgaacgcct tatccgacct acgtgtgacg tgtaggcctg
    36661 ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac ttactgctgt
    36721 gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc acgcggcgac
    36781 aattcaccgg caccaatggc tttttccaga ctctggctaa cggtcgtatg caccgcctga
    36841 gcggagtggt catcaccact ttccagttct gcgatggcta atgtcaggtg gccacgcaaa
    36901 tacccactgg caaacaactc atcatcactg gcatggtcaa ccataccgtc gattaatgcc
    36961 agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac gtggcgctgg
    37021 cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg cgtgtgataa
    37081 taattttgta gtgctttaat gaagcgtgcc gggcgttccg gaatgccatt tgttagataa
    37141 tccatcacct gcgcatgtac tcgccgttga aacaccacgc ggtccggttc gaagtcgcct
    37201 tccagattgt cgcaactgac gttaaacgga tatccagccg ccacgcagaa caaccagtcg
    37261 agcgcctgcg gcttcacttc aacatcttca aactggcttt gcgtttgggc atcgcgtccg
    37321 tccgggcaat accagtagcc gaaatcaacc agctcacggc gcgcttttcc ggcaatacac
    37381 cagtgcgaaa tctcatgaat tgcgctggca taaaagccgt gggcaaagac gattcggtta
    37441 tacggtactt ccacatcagc aggaagatag atcggttcgt cgtcgccttt aatcagacgg
    37501 gtattaaaat catcggcaaa gcagctatta aaaatttcaa tcaactgctc atagtggtgt
    37561 gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg ctatcataaa
    37621 gtaatttggc actcatcacc gccgagacga taacgatcat cgggcggatc agtttttgtc
    37681 ctttgctcaa caccagtctt gaacccatgc gtgcgccgag gaactgcccc accagcatca
    37741 caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagtaaa ccgccgatgt
    37801 ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca
    37861 gcgtaacaaa ggccagcgcg taaaacgatc cggctgccgg accaaagaat ccgtcataaa
    37921 aaccgacgca gccaccggca attaacgcga aaggtaaacc gtacatccgg cgctggcgat
    37981 cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc accagaatgg
    38041 gcaaaatctg ccgtaagaca tcagcctgaa cgtattgcac cagcagtgcg ccgctcattg
    38101 agccgataaa ggtcatggcg atattgagtt tctgatcact taaactaacc actttgcggc
    38161 gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta ttggttgcca
    38221 gcgcattagc gggagacatc cccgctgcca ttaatgccgg aatcgtgagt aacccaccac
    38281 caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga actcccagca
    38341 acagcgggga aaccataaac aggctattaa acgtttccat tagatcacgt gctcatccag
    38401 tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc
    38461 cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc ctggtggcgg
    38521 taaaggttga tcttcacact ccagactatc ggcaggacaa cgtaatcgta catgcatatg
    38581 cgcacgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc
    38641 aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg
    38701 ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga caacgtgttt
    38761 accgtcgcgg gatactaagt ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg
    38821 agttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc tggcatgacc
    38881 gccgttgaaa cgcccaccag cgggcatccc catatcgcca atcagcaccg tacccatgcc
    38941 cagattgctc acctggctac tcagacgctg gataaacatc accaggtccg ggtgaccgaa
    39001 atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg gcagcgtgtc
    39061 agcgccgacg atacagccat tagaaaaact gcctatcgat tgtgcgctac ccggcacagg
    39121 ttgggttatt ttttgccacg gcgtcgctgc caagctgacg ctactggcaa gcagggccag
    39181 cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct tcacatcggc
    39241 attttgcgcc cgctgccgta acaggtgatc cattaatacg atcgccagca tcgcttcagc
    39301 gatcggcacc gcgcggatcc cgacacaggg atcgtgacgg cctttggtga tcatctcaac
    39361 ttcttcgcca aagcggttaa tggtgcgccc cggcacggta atgctggaag tcggtttcag
    39421 cgccatatgg gcaatgattt gctgcccgct gctgataccg ccaagaatgc cgcccgcatg
    39481 gttgctctgg aaaccgtctt tggtgatttc gtcgcgattc tggctgccgc gcagcgccac
    39541 cacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg
    39601 ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca agtccggcgg ggacaccact
    39661 ggctacaacg gtgactttcg cgccgatgga gtcgccctct tttttcagcg cgcgcatcag
    39721 ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag aacggatttt gttcaacctg
    39781 cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc
    39841 aataccaaat gtctcggcga gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc
    39901 ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt atttttgttc
    39961 gtaggtgtaa tcagcatggc caggacggaa aacgtcctta atcgcactat aatcctgaga
    40021 acgctgatcg gtattttcga tcaacaaacc aatgctggtg ccggtagtaa caccttcaaa
    40081 aacaccggag agaattttga cctgatctgg ctcacgacgc tgtgtggtat agcgcgatgt
    40141 cccagggcga cgacgatcaa ggtcatgttg caggtccgct tccgtcagcg gaatgcccgg
    40201 cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga aggtggttac
    40261 gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt tgtgtttgcg
    40321 tgtttactta atctttataa atcgcgaaat gttcacgggc cgccagcagc tgctctttgg
    40381 tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac ggaacatccg
    40441 gatattgttc cataagatgt accatgctgt tgccgacttc acaaatcagc acaccatcat
    40501 cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt cagtttcagg ccgtcagtgc
    40561 cagatgccag gcccagttcc ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt
    40621 ccgcatcgac atacggcggg ttagtgacga tcaggtcgta ctgcactttc ggcaaatcac
    40681 ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag accgtgttct tcgatgttct
    40741 gttcggcaac cgccagcgcg tcgggagaga tatccaccgc gtcgacttcc gcatccggga
    40801 aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct aaaatatgct
    40861 gcggttgctt gctgattagc ccggcaaatt tattgttaat cagttcacca atcggcgaac
    40921 gcggcaccag cacgcgttca tcgacgtaaa attcatggcc gcagaaccag gctttattgg
    40981 tcaggtaagc caccggaatg cgttcgttga cgcggcggat cacgcgctca acaatacggt
    41041 gtttttcgct ggaggtcaga cgcgcagtgc gcatatcttc cggaatatcc agcggcaggt
    41101 agagcgacgg caacaccagt tgtaccgctt catcccacgg gttatcggtg ccgtgaccgt
    41161 accagatatt tgccgcgctg aagcggctaa ccgaccagcg caacatgtcc tgaatggttt
    41221 gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc agggcatgat
    41281 cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca ttcacgcgcg
    41341 gtgagtgagg aaaaatacgt ttaaaacgat cgattgcgct acgagtcggg taaactgtag
    41401 gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg aggaggacca
    41461 ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca cgattgtcca
    41521 ccgaccgcag cgtaagaaaa tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc
    41581 tgatgccagc cattatttct ccgatgagtt tcagccgtta ttaaataccg aaggcccggt
    41641 gaaatatgtt cgcccggatg tgagtcattt tgaggcgaag aagttgcgac gcggtgatta
    41701 ttcgccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca agcaggaact
    41761 gggcgcgttg attgccgcct gccgtcgtga acatgtgttt tgcgcatgcg taatgcacgg
    41821 gcacggaaag catattttga aacagcaaac gccgctgtgg ctggcgcaac atccgcatgt
    41881 aatggcattt caccaggcgc cgaaagagta cggcggagat gcagcgttgt tagtgttgat
    41941 tgaagtggaa gagtggctac cgccggagtt gccgtgaata ttagggggcg gatgcgcttt
    42001 gccatttcgc cctgaatgct gggaccaaat gccggatgcg gggtaaacgc cttatccggc
    42061 tacgaacact gtcgaggcta ataatattaa ttaaagttca ttaagaatat attgaaagcg
    42121 acatgtagca tcaaaacttt gccgttcact agtatcaaca cgcacctgca tgtcatggag
    42181 ttgtttatca gttacaggat gtcggtttgc cgttacgttc actttattgt tatcacaacc
    42241 ctggacacta aacgtcatta attgtgatac caggcgacaa taggctccac ggtcatcact
    42301 gaagtcactg ccatcacata atcgcccatc tttcattaag aatgtcggca acgatttctc
    42361 aataaccccc cccgtagatt gcaggcttaa tgatttatta tgaacaacgg tgttttcatc
    42421 ccaggaaata tcatagcttg ctactggctc tgttggacaa tttgattggt gcgcattcgg
    42481 cccctgataa taatatggcc ccgcctgcgg aggccttgct gtgctataaa ttgatgttgt
    42541 caccacccag gggcaaccat gggttgtacc cgtaacatgt acatttacag gaagtctatg
    42601 acaaacccaa ccagaggtca taccgggaca ataagaatca tctactatcg gtgcccgccc
    42661 tggcggaatt gttatgtctg gggggacacc cggtccagtc caggttaagt taggcacaac
    42721 tcctgcctgt accataatcc ccccttgtga atatgtccca taatatgccc cttcaggggt
    42781 taacacatct attttataaa cgatagatgt tgttagccca ctgccataag atgcgccata
    42841 agtctgaaca ttattaaatg taacgtctgc ttgt
//
LOCUS       DAZUGK010000017        39546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZUGK010000017 DAZUGK010000000
VERSION     DAZUGK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.017"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..997)
                     /gene="yeiR"
                     /locus_tag="ACHY5W_000805"
     CDS             complement(11..997)
                     /gene="yeiR"
                     /locus_tag="ACHY5W_000805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFS4445752.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLSRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1115..2581)
                     /locus_tag="ACHY5W_000806"
     CDS             complement(1115..2581)
                     /locus_tag="ACHY5W_000806"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4445753.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(2804..3376)
                     /gene="yeiP"
                     /locus_tag="ACHY5W_000807"
     CDS             complement(2804..3376)
                     /gene="yeiP"
                     /locus_tag="ACHY5W_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFS4445754.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3531..3785
                     /locus_tag="ACHY5W_000808"
     CDS             3531..3785
                     /locus_tag="ACHY5W_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS4445755.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3782..4963)
                     /gene="setB"
                     /locus_tag="ACHY5W_000809"
     CDS             complement(3782..4963)
                     /gene="setB"
                     /locus_tag="ACHY5W_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFS4445756.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            5331..6461
                     /gene="fruB"
                     /locus_tag="ACHY5W_000810"
     CDS             5331..6461
                     /gene="fruB"
                     /locus_tag="ACHY5W_000810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFS4445757.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDASFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            6461..7399
                     /gene="fruK"
                     /locus_tag="ACHY5W_000811"
     CDS             6461..7399
                     /gene="fruK"
                     /locus_tag="ACHY5W_000811"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4445758.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7416..9107
                     /gene="fruA"
                     /locus_tag="ACHY5W_000812"
     CDS             7416..9107
                     /gene="fruA"
                     /locus_tag="ACHY5W_000812"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFS4445759.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVVKAA"
     gene            9531..10472
                     /locus_tag="ACHY5W_000813"
     CDS             9531..10472
                     /locus_tag="ACHY5W_000813"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFS4445760.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSEISGESGWSGSIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            10460..11398
                     /gene="psuG"
                     /locus_tag="ACHY5W_000814"
     CDS             10460..11398
                     /gene="psuG"
                     /locus_tag="ACHY5W_000814"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFS4445761.1"
                     /translation="MSELKISPELLQIFPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPQQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            11492..12741
                     /locus_tag="ACHY5W_000815"
                     /pseudo
     CDS             11492..12741
                     /locus_tag="ACHY5W_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(12809..13507)
                     /locus_tag="ACHY5W_000816"
     CDS             complement(12809..13507)
                     /locus_tag="ACHY5W_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFS4445762.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLYYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            13637..14578
                     /gene="rihB"
                     /locus_tag="ACHY5W_000817"
     CDS             13637..14578
                     /gene="rihB"
                     /locus_tag="ACHY5W_000817"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFS4445763.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDRIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            14678..15928
                     /locus_tag="ACHY5W_000818"
     CDS             14678..15928
                     /locus_tag="ACHY5W_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS4445764.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(15984..17072)
                     /locus_tag="ACHY5W_000819"
     CDS             complement(15984..17072)
                     /locus_tag="ACHY5W_000819"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS4445765.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGAINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17075..17932)
                     /gene="nfo"
                     /locus_tag="ACHY5W_000820"
     CDS             complement(17075..17932)
                     /gene="nfo"
                     /locus_tag="ACHY5W_000820"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4445766.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18006..19055)
                     /locus_tag="ACHY5W_000821"
     CDS             complement(18006..19055)
                     /locus_tag="ACHY5W_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFS4445767.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19154..20035
                     /gene="yieE"
                     /locus_tag="ACHY5W_000822"
     CDS             19154..20035
                     /gene="yieE"
                     /locus_tag="ACHY5W_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFS4445768.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20240..21709
                     /gene="lysP"
                     /locus_tag="ACHY5W_000823"
     CDS             20240..21709
                     /gene="lysP"
                     /locus_tag="ACHY5W_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFS4445769.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22002..23993
                     /gene="cirA"
                     /locus_tag="ACHY5W_000824"
     CDS             22002..23993
                     /gene="cirA"
                     /locus_tag="ACHY5W_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFS4445770.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNIEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24025..24861)
                     /gene="yeiG"
                     /locus_tag="ACHY5W_000825"
     CDS             complement(24025..24861)
                     /gene="yeiG"
                     /locus_tag="ACHY5W_000825"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS4445771.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNHENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25119..25787
                     /gene="folE"
                     /locus_tag="ACHY5W_000826"
     CDS             25119..25787
                     /gene="folE"
                     /locus_tag="ACHY5W_000826"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFS4445772.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            25804..26961
                     /gene="yeiB"
                     /locus_tag="ACHY5W_000827"
     CDS             25804..26961
                     /gene="yeiB"
                     /locus_tag="ACHY5W_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFS4445773.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSETSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27103..28143
                     /gene="galS"
                     /locus_tag="ACHY5W_000828"
     CDS             27103..28143
                     /gene="galS"
                     /locus_tag="ACHY5W_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFS4445774.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRIVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            28423..29421
                     /gene="mglB"
                     /locus_tag="ACHY5W_000829"
     CDS             28423..29421
                     /gene="mglB"
                     /locus_tag="ACHY5W_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFS4445775.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAAETRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            29482..31002
                     /gene="mglA"
                     /locus_tag="ACHY5W_000830"
     CDS             29482..31002
                     /gene="mglA"
                     /locus_tag="ACHY5W_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFS4445776.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31018..32028
                     /gene="mglC"
                     /locus_tag="ACHY5W_000831"
     CDS             31018..32028
                     /gene="mglC"
                     /locus_tag="ACHY5W_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFS4445777.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32271..33506)
                     /gene="preA"
                     /locus_tag="ACHY5W_000832"
     CDS             complement(32271..33506)
                     /gene="preA"
                     /locus_tag="ACHY5W_000832"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFS4445778.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWVDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(33500..34738)
                     /gene="preT"
                     /locus_tag="ACHY5W_000833"
     CDS             complement(33500..34738)
                     /gene="preT"
                     /locus_tag="ACHY5W_000833"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFS4445779.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(34932..35171)
                     /locus_tag="ACHY5W_000834"
     CDS             complement(34932..35171)
                     /locus_tag="ACHY5W_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFS4445780.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35174..35893)
                     /gene="sanA"
                     /locus_tag="ACHY5W_000835"
     CDS             complement(35174..35893)
                     /gene="sanA"
                     /locus_tag="ACHY5W_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFS4445781.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36043..36927)
                     /gene="cdd"
                     /locus_tag="ACHY5W_000836"
     CDS             complement(36043..36927)
                     /gene="cdd"
                     /locus_tag="ACHY5W_000836"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4445782.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37057..37752)
                     /locus_tag="ACHY5W_000837"
     CDS             complement(37057..37752)
                     /locus_tag="ACHY5W_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFS4445783.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(37749..38147)
                     /locus_tag="ACHY5W_000838"
     CDS             complement(37749..38147)
                     /locus_tag="ACHY5W_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4445784.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            38386..39333
                     /gene="dusC"
                     /locus_tag="ACHY5W_000839"
     CDS             38386..39333
                     /gene="dusC"
                     /locus_tag="ACHY5W_000839"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFS4445785.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         9641 a         9724 c        10461 g         9720 t
ORIGIN      
        1 gcaggcaacc ttacgcggta gtcgctaaac gaagcttcaa cagcgcgctc tgcaaggcat
       61 tccagtcagc ttcgctgctg gaaatcagct caatacggct gtccggtggc gcaacgtttt
      121 gcgtttcaat gtgcaggtca tcgccctgac ggttgattcg caccagccct tcgggaatac
      181 gcagcacgcc tttgacgcgt tccaccggtg caagtcgcgc ccattccaga atgccaatgg
      241 tgtcgaatac cgtatcagcg tcgaatatcc agccgcaggc ctgatatcct tgcccgctgt
      301 tcagactgcg acgccagcgt tgatgctctg gcaggcttaa cgctgctaac ccttttttca
      361 cgacatgctg atgagaatgc gcggcgctgg cgggcaactc ggctaaattg cgacgcgaca
      421 aatccagaag atgaccgtca actttcccat gctcactgtg aattaattgc cgatcgccac
      481 cattttgctg ccaccagcgc tgtagcgctt gctcgctttc aggtgtcgca cggtcggatt
      541 tattggcgac aatgatgtct gcggcagcca gctggtcacg gaagttttca ttgctggcgc
      601 ttttttcgtc cagcagcagg cgtggatcga gaatgcacaa cgtggcgcgt agatctatcc
      661 acggttcata gactggtgcg gttaagagat cgaggatctg tttcggatgg cccagcccgg
      721 tcggctctat caacaagcgg tctggttttc cctgacgcag taaggtattc aaccctacct
      781 gcatgggtaa accattaacg cagcacatgc agccgccggg gatctctttc agcaatgcgc
      841 cgctatcggc gagcaaagca ccatcaattc cgacttcccc aaattcatta accagtaccg
      901 cccatttttc gttgggatct ttatgggcta acagatgaag aatcgacgta gttttcccgc
      961 tgccgagaaa accggtgatg aggttggtcc tggtcacaac ggctccgcaa tactctgata
     1021 agttaactga gcgttaatcc tggctaaaaa cggcagaaaa gagaagggga gagcggacga
     1081 aggcgagctt catccgcttt aaccgcagaa atcgttaaat tttgagggtg tttaatagcg
     1141 cctgatgtgc gccgaattgt acgatttgtt gccaggcttg ttcgatggcc tgcacaaaat
     1201 gcgggttatc tggcagatca tcaccgaaaa cttcacgcag ggaaagcagg gcggttacgc
     1261 gttgttcact actgctgccc gcaacaagtt cgcgaatttt atcgctaagc ggatcgcgaa
     1321 catcaatggc atttccggca tcatcaacgc cgctgacgta acgcatccag cctgcaacgc
     1381 ctaatgccag caacgaccag tccgtttcgc gccccagatg tatgcgaata cctgccagca
     1441 tgcgttgcgg taatttctgg ctgccatcca ttgcgatttg ccaggtctta tgtttcagcg
     1501 ccgggttagc aaaacgtgca attaacttat ccgcatattg tgttaaatcg acatctttaa
     1561 ttcgcagtgt cggcgcttgc tcattcagca ttaatgttct ggcggcatgg cgaaatgcgc
     1621 gatcctgcat gcaatcactg atatgggcga atcctgagag gtaacccaga taagcgagaa
     1681 aagagtggct gccattaagc atacgcagct tcatctcttc ccatggcagg acatcattca
     1741 ccatttgtac acctgcgact tcccaggcag gacgcccagc gacgaagtta tcttccacca
     1801 cccactggat aaacggttcg cagctaatcg cgcagggatc attcaccccc agatgctggc
     1861 tgatttctgc cagtgattcg tcggttgcag ccggaacaat gcggtcgacc atggttcccg
     1921 gaaaactgac gtgttctttt atccacccgg cgagttctgg cgaacgtttt tctgccattc
     1981 ccagcaccgc gtttttcacc acatggccat tgtcgggaat attgtcgcag gagagcacgg
     2041 taaacggtgt aaggccgcgc tcacggcggc gtttcagtgc ttcgacgaga atacctggcg
     2101 cggagtgagg ttcttcaggg gtttgtagat cgtgaataat ccgcggattg ctggtgtcga
     2161 gtgcaccggt ggcagggtca atacaatagc ctttttcggt aatcgtcagg gaaacaattg
     2221 ccacctgtgg ctcgcaaaac ttctcaataa ttgccgctaa ggaatccagt tttgcattca
     2281 ggcattcgtg aacggcaccg acaattatcg cctgattgcc gtccgcccct ttctccagca
     2341 cggtatataa atggttctgt gcgcggagct ggctcatcag ttgatcaccg ctgaacaagc
     2401 tgatttcaca gatcccccag tcgccgccct gggcattcag cacacgatcg gtcagtaacg
     2461 cctgatgtgc gcggtgaaag gctccaaagc caaaatgaac gatacgtgat tgcaactgct
     2521 ggcgatcgta gcgtggagcg tgtacatgat gcgggagcgt aacggaggcg attgtattca
     2581 taggagtcac gtcttggtaa cgattaatta acaatgacag tgtaaagtta tatgacagca
     2641 attgaaattg gtctggattg ttaatgtgat gaagtgtgag ggcgatcaat caacgagtga
     2701 atattgcggg cgtaaggtgt tcaggaagcg caaaatagaa gattggtata ataaattaaa
     2761 aaagggacag cctgagctat cccttttctg catgcggctg aagttagtca gcacgcccca
     2821 tataacggcg ttcttcgata tggatacgaa ttttttcgcc cgggcttaag tattctggta
     2881 cctgaatcac cagaccagtg ctcaatgtcg ccggtttgtt acgggcgctg gcagatgccc
     2941 ctttgatgcc tggtgcagtt tcaacgattt ccagatcaac ggtctgcggc agctcaagcg
     3001 ccagcagttg accgtcccag gtcagcacct gcatgtccgg catgccgccc tctggcataa
     3061 acagcaactc ttcttcaatc tgatctttgg tgaaggtata cggggtatag tcttctttat
     3121 ccataaagac atattcgttg ccatcgacat aggagaagtc aacgtaacgg cgggtcagcg
     3181 tcacggtgtc aacgatatca tctcctttga aacgctcttc tactttcagc ccggtacgga
     3241 catcagaaaa acgcattttg tacagcgtag cggcaccgcg ggcagtgggc gactgaatat
     3301 caatgtcctt aaccagcagc agtttgccgt tgtaattcag taccatacct tttttaattt
     3361 cgttcgctct tggcatcgaa aaattcctgt agtcgaaaat gtcaaaaata tcgcgacaaa
     3421 gtactcgcga cgggcctttc gggcaagtgg tattcgcact tttgctggtg caaaaaaggt
     3481 ggtactgtgc gcgctcatca atccggtggt taaccttaag agaacaacgt atggaatgcc
     3541 gtccgggttg cggggcgtgt tgcactgccc cttcaatttc cagccctatt cccggcatgc
     3601 cagatggcaa gcccgccaat acgccctgca ttcagcttga tgaacagcaa cgctgtaaaa
     3661 ttttcacctc gccactgcgg ccgaaagtct gtgcaggctt acaagccagc gctgaaatgt
     3721 gcggtaactc gcgtcagcag gcaatgacct ggcttatcga tctggaaatg ctgaccgcgc
     3781 cttaaacatc tttaatccgc agtaagcaaa acagagtggc gataatcatc accatcgcaa
     3841 accagaacac agcgtgataa ttccagatct cggcgacgat gcccgctaca gatcctgcga
     3901 tgatccagcc cacgcgcgac gtgttggtat agagcgtggt ggctgaacct gcctgaccgg
     3961 gcatcaaatc ctgaaaatag agcatcccga tgccgcccag aatgccaata aaaatagcat
     4021 ttagcagctg caagcccaac agaatggcag gtgaatgcgc catcagcatt cctgcgtaaa
     4081 aacagacgcc acccacggca gcaacgcgca ttaagaaacg cttacccaga cgtttggcaa
     4141 aatatccggc aatcaacatg gtcgggattt ccagcccggc ggcggtcccc atcatcacac
     4201 cggccagttt ctcgggaaga tgcagttcgt tgattataaa tagcggcatg ttgatgatgt
     4261 acagactgtt cgagccccac atcaatgtac aaatgacaaa cagcagcagc gtatcgcgac
     4321 ggttacggcg cggcgcttca accgtgccgg tcgccagcgg aagctctttt tgcatcgacg
     4381 gtaaaaacag ccacaccatc acgccgcaaa caataaacgc taccgctgcg ctcagataca
     4441 ttaccgtaaa gctgaaaccc atcgctaagg cataagccag cggtgggcca atgacccatg
     4501 ccagtgaaac ctgagcgcgt aaaaaagagc tgaacatcac cgcctcacgt ccggttttgt
     4561 cggcatgttc acgggcaagg gcaaacattt gcgggttagc ggtcgagcca aagctgctaa
     4621 gaaagacgcc aacgaatagc aaaacaaagt agttgcgatt ccaggcaaaa agggtgcagg
     4681 ccagcacgcc taacaggcag caaaagacaa tcagcgattt gcgatcgccg cgcttatcag
     4741 agcgcccggc gagaaactga ctcaccagaa tcccaatgac agcgctgccg gtaaagaaga
     4801 atcccaccat cgccggacgg gcatgtactt catcggtaag aaaaatactg agtgtcgggg
     4861 tttgcagagc gcccgcaata ccggtgagaa aggcaacgat taaaaacgcc gtcgaggtca
     4921 ggtcaaacga tttcgcgctg gagactgcgg gggagttatg catgctggtc gatcactgga
     4981 agaggaagac gcggagttta cgctgcttgt cagaaaataa acagacagcg aaatcaaaag
     5041 cgccacgaat aatcaaaatg ccatttcact gttgctgatt ggctgctgac gctgctgaaa
     5101 tgaaattgct gataacggat tttcccatca gcaattcgga aaaatgacaa aaaattgtgc
     5161 agcacatcaa acttttgctc ataactttac ggctttcctt gcgtgctgaa acagaagtat
     5221 tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca ggttggcgct
     5281 ttttttctca tagaagctga atcgtttcaa ttcagcaaga gaggagaact atgttccagt
     5341 tatccgtaca ggacatccat ccgggcgaaa aggccggaga caaagaagag gcgattcgcc
     5401 aggtcgctgc ggcgctggtg caggccggta atgtagcaga aggctacgtc aatggcatgc
     5461 tggcgcgcga acagcaaacc tcaacgttcc tcggcaatgg tattgctatt ccacacggca
     5521 ctaccgacac ccgcgatcag gtgctgaaaa ccggcgttca ggtatttcag ttcccggaag
     5581 gcgtcacctg gggtgacggt caggtagcgt acgtagcgat cggtattgct gccagctcgg
     5641 atgagcatct gggcctgcta cgccagctga cccacgtact gagcgatgat tccgttgctg
     5701 aacaactgaa gtcagcaaca acagcagaag aacttcgcgc attgctgatg ggcgaaaagc
     5761 agagtgagca gttgaagctc gacaacgaaa tgctgacgct ggatatcgtc gccagcgatc
     5821 tgctgactct tcaggcgctg aacgctgcgc gtctgaaaga ggcgggggca gttgacgcct
     5881 ctttcgtcac caaagccatc aatgaacaac cgctgaatct cggacagggt atctggctga
     5941 gcgatagcgc cgaaggcaat ctgcgtagcg cgattgcggt aagccgtgcg gcaaatgctt
     6001 ttgatgtgga cggcgaaacg gcagccatgc tggtgagtgt ggcgatgaat gacgatcagc
     6061 ccattgcggt tcttaagcgt ctcgctgatt tgttgctcga caataaagct gaccgcttgc
     6121 tgaaagcgga tgcggcaacg ttgctggcgc tgctgaccag cgatgatgcg ccgaccgacg
     6181 acgtgttaag cgcggagttt gtggtgcgca atgaacacgg cttgcatgct cgtccaggta
     6241 ccatgctggt caataccatt aaacaattta acagtgatat taccgtgaca aatcttgatg
     6301 gcaccggcaa accggcaaac ggacgtagtc tgatgaaagt tgtggcactt ggcgttaaga
     6361 aaggtcatcg cctacgcttt accgcccagg gtgcagatgc tgaacaggcg ctgaaagcaa
     6421 tcggcgacgc tatcgctgct ggtcttgggg agggcgcata atgagcagac gtgttgctac
     6481 tatcaccctt aatccggctt atgaccttgt tggtttctgc ccggaaattg aacgcggcga
     6541 agtgaacctg gtgaaaacca ccggtctgca tgcggcgggt aaaggcatca acgtggccaa
     6601 agtattaaaa gacctgggaa ttgatgtcac cgttggcggc ttcctgggta aagacaatca
     6661 ggatggtttt cagcaactgt tcagcgagct gggcattgcc aaccgtttcc aggttgtaca
     6721 ggggcgcacc cgaattaacg ttaagctgac ggaaaaagac ggcgaagtga ccgacttcaa
     6781 cttctcgggt tttgaagtca ctcccgccga ctgggaacgc tttgtgactg attctctgag
     6841 ctggctcggt cagttcgata tggtctgtgt cagcggaagc ttaccgtcag gcgtcagccc
     6901 ggaagcgttc accgactgga tgactcgcct gcgtagtcag tgcccttgca ttatctttga
     6961 tagtagccgt gaagcgttag tagcaggttt gaaagcggca ccgtggctgg tgaaacctaa
     7021 ccgccgcgag ctggaaatct gggcaggccg taaactgcct gaaatgaaag atgtgattga
     7081 agctgcgcat gcgctgcgtg aacaaggtat tgcgcatgtc gttatttcac tgggtgccga
     7141 aggcgcgctt tgggttaatg cctccggcga atggatcgcc aaaccaccgt cagtcgatgt
     7201 cgtaagcacc gttggcgcag gggattctat ggttggtggc ctgatttatg gcttgctaat
     7261 gcgtgaatcc agtgaacaca cactgcgtct ggcgactgct gttgcagccc tggcggtaag
     7321 tcaaagcaat gtgggtatta ccgatcgtcc gcagttggcc gcaatgatgg cgcgcgtcga
     7381 cttacaacct tttaactgac agcaggagag gcataatgaa aacgctgctg attattgacg
     7441 ctaatctcgg tcaggcacgc gcctatatgg cgaagaccct gctgggcgcg gcggcgcgaa
     7501 aagcaaaact ggaaatcatc gacaatccga acgacgcaga aatggcgatt gttctcggtg
     7561 attccatccc gaatgacagc gcgctgaacg gtaaaaatgt ctggctgggc gatatttccc
     7621 gggcagttgc gcaccctgag ctgttcctga gtgaagccaa aggccatgcg aaaccttaca
     7681 ctgcgccggt cgctgcgaca gcaccggttg ccgccagcgg tccgaaacgc gtagttgcgg
     7741 tgactgcttg cccgactggc gtagcacaca cctttatggc ggctgaagcc attgaaaccg
     7801 aagcgaaaaa acgtggctgg tgggtgaaag ttgaaacccg tggttctgtt ggtgcgggta
     7861 atgcaatcac ccccgaagaa gtagccgcag cggatctggt gattgtggcg gcagatatcg
     7921 aagtggatct ggcgaaattt gctggtaaac cgatgtatcg tacctctacc ggtctggcgc
     7981 tgaagaaaac cgcgcaggaa ctggataaag cggttgctga agcaacgccg tatgaaccgg
     8041 cgggcaaagc tcaaacggcg acctctgaag gtaagaaaga gagtgcaggc gcataccgtc
     8101 acttgctgac gggcgtctct tacatgctgc cgatggtcgt agcaggtggt ctgtgtatcg
     8161 cgctttcttt tgcttttggt atcgaagcgt ttaaagagcc gggtacgttg gcagcggcgc
     8221 tgatgcagat tggtggtggt tcagcctttg cgctgatggt gccggtactg gcaggttaca
     8281 ttgccttctc cattgccgat cgtccgggtc tcacgccggg tctgattggc ggtatgctgg
     8341 cggtcagcac cggttctggc ttcattggcg gtattattgc gggcttcctg gctggttaca
     8401 ttgcgaagtt aatcagtacg caactgaaac tgccacagag tatggaggcg ctgaaaccga
     8461 tcctgatcat tccgctaatt tccagtctgg tggtcggtct ggcgatgatc tacctgatcg
     8521 gtaaaccggt tgctggcatt ctcgaagggt tgactcactg gctgcagacc atggggactg
     8581 cgaatgcggt tctgctgggg gcgatcctcg gtggcatgat gtgtactgac atgggcggtc
     8641 cggtaaataa agcagcgtac gcattcggtg tgggtctgct gagtactcaa acctatggcc
     8701 cgatggcggc gattatggcg gcaggtatgg tgccaccgct ggcaatgggt ctggcaacaa
     8761 tggtggcgcg tcgcaaattc gacaaagcgc agcaggaagg tggcaaagcc gctctggttc
     8821 ttggtctgtg ctttatttcg gaaggtgcaa ttccgtttgc tgctcgtgat ccgatgcgtg
     8881 tgctgccgtg ttgtatcgtt ggtggggcgc tgactggcgc aatctcaatg gcgattggtg
     8941 cgaaactgat ggcaccgcac ggtggtctgt ttgttctgct gatccctggc gctattacgc
     9001 cggtactggg ttacctggta gcaattattg ccggtacgct ggtggcgggt ttggcctatg
     9061 ccttcctgaa acgtccggaa gtggacgcag tagtgaaagc cgcgtaataa aaggtgtgtt
     9121 acagggcaga aatttactgc cctgtatcat tggatgagcg caaagagaat gaccggtttg
     9181 tgaatgtaat tttatgaatt taaatggttt tatctttcag tagaaacctg aaacgttcat
     9241 caaacactaa ggttatttcc tcaagggcag attcttttct gccctttttt agtgttcatg
     9301 ttctgtgcat cccgtcacaa attcctcgct tttgtaattc aggcatttaa tcgcattttg
     9361 tgagcctgct caaatctgcc cgctttgcct ctctggcggc aatttacttt acgtaaaaca
     9421 tgcatactat gagcacatgt ttaaatatga acacatgttt aaaccgtctt tgcagtatct
     9481 atattcttgt ggattgacag ggttggtaat tttttaccag aggacgtgct atgcgcgaaa
     9541 aggattatgt cgtaattata ggctcggcga atattgatgt cgccggatat tcacatgaat
     9601 cattaaatta tgcggattca aatccaggta aaataaaatt tacgcctggt ggggtagggc
     9661 gcaatattgc acaaaacctg gcgttgctgg gtaataaagc ctggctactg agcgccgtag
     9721 gcagtgattt ttatggacaa tcgctgctaa cgcaaaccaa tcaatctggc gtttatgtcg
     9781 ataaatgcct gattgtgccg ggagaaaata cgtcgagcta tttatcatta ctcgataata
     9841 ccggtgaaat gctggttgct ataaatgaca tgaatattag caacgctatt acagctgaat
     9901 atctcgcaca gcaccgtgaa tttattcaga gggcaaaggt cattgtcgcg gactgtaata
     9961 tcagtgaaga ggcactggca tggattctgg ataatgctgc caacgtaccc gtatttgtcg
    10021 atccggtttc agcgtggaaa tgtgtcaaag tgcgcgaccg tctaaatcag atccacactc
    10081 tgaagccaaa ccgccttgaa gcggaaaccc tgagtgggat tgcgctgtca gggcgtgaag
    10141 atgtggcaaa agttgctgcc tggttccatc aacatggcct gaaccgactg gtattgagca
    10201 tgggcggcga tggcgtttat tacagtgaaa tcagcggtga aagtggctgg tctggctcga
    10261 tcaaaaccaa tgttattaat gttaccggag cgggcgatgc catgatggcg ggacttgctt
    10321 cgtgttgggt agacggaatg ccgtttgccg aatctgttcg tttcgcacag ggatgttcgt
    10381 caatggcgct ctcctgtgaa tacaccaata accccgattt atcgattgcc aacgttatat
    10441 cgttagtgga gaacgcagaa tgtctgaatt aaaaatttcc cctgaattat tacaaatttt
    10501 cccggaagtg caggacgctt taaaaaacaa aaaaccggtt gtggcgctgg aatcgaccat
    10561 tatttctcac gggatgccgt tcccacaaaa tgcccagacc gcaattgaag tagaagaaac
    10621 tattcgtaaa cagggcgcag tacctgccac tatcgccatt attggcggcg tgatgaaagt
    10681 gggtttaagc aaagaagaaa ttgaattact gggtcgtgaa gggcataacg tgaccaaagt
    10741 tagtcgtcgc gatttacctt ttgttgttgc cgccggaaaa aatggcgcaa ccactgtggc
    10801 ttcaacgatg attattgcgg cgcttgccgg aattaaagta tttgccaccg ggggaattgg
    10861 tggtgtgcat cgcggggcgg aacatacctt cgatatttct gccgatttgc aagaactggc
    10921 aaatactaat gtcaccgttg tttgtgccgg ggcgaaatct attctcgatt taggattaac
    10981 cactgagtat ttagaaacct tcggtgtgcc gttaattggc tatcagacta aagcgctgcc
    11041 tgcgtttttc tgccgcacca gcccgtttga cgtcagcatt cgtctcgaca gcgccagcga
    11101 aattgcccgt gcaatggcgg tgaaatggca aagcgggctg aacggtggcc tcgtggtagc
    11161 gaacccgatc ccgcaacagt ttgctatgcc ggaacacact atcaatgcgg cgatcgatca
    11221 ggcggtagct gaagctgaag cgcagggtgt tattggtaaa gaaagtacgc cattcctgct
    11281 ggcgcgcgtt gctgagctga ccggcggtga cagcctgaaa tccaacatcc agctggtgtt
    11341 caacaacgcc attctggcga gcgaaattgc caaagaatat cagcgtctcg cgggttaatt
    11401 tctgacgggc agaaagccgt cgcccgtact gatcatacat accgaaacct ggcgctgacg
    11461 ccgggtttac tggcatgaag ggaaaatcat tatggatata atgagaagtg ttgtggggat
    11521 ggtggtgtta ctggcaatag catttctgtt gtcggtaaat aaaaagagca tcagtttgcg
    11581 cacggttgga gccgcactgc tgctgcaaat cgctattggt ggcatcatgc tctattttcc
    11641 gccgggaaaa tgggcagtag aacaggcggc attaggcgtt cataaagtga tgtcttacag
    11701 tgacgccggc agtgccttta tttttggttc attggtcggg ccgaaaatgg atgtcctgtt
    11761 tgatggcgca ggctttatct tcgcctttcg cgtactcccg gcgattatct tcgtcactgc
    11821 gctcattagt ctgctgtact acattggcgt gatggggctg ctgattcgca tccttggcag
    11881 cattttccag aaagccctca acatcagcaa aatcgaatct tttgttgcgg ttaccactat
    11941 tttcctcggg caaaatgaga tcccggcgat cgttaaaccg tttatcgatc gcatgaatcg
    12001 caacgagttg tttacggcta tttgtagcgg gatggcgtcc attgcgggtt cgatgatgat
    12061 tggttatgcc ggaatgggcg taccaattga ctacctgttg gcggcatcgc tgatggcgat
    12121 ccccggcggt attttgtttg cacgtattct tagtccggca accgagcctt cgcaggtcac
    12181 gtttgaaaat ctgtcgttca gcgaaacgcc gccaaaaagc tttatcgaag cggcggcgag
    12241 cggtgcgatg accgggttga aaatcgccgc cggtgtggcg acggtggtca tggcgtttgt
    12301 cgcaattatt gcgctgatta atggcattat tggcggaatt ggcggctggt ttggttttgc
    12361 caatgcctct ctggaaagta tttttggcta tgtgctggca ccgctggcgt ggatcatggg
    12421 tgtggactgg agtgatgcca accttgcggg tagcctgatt gggcagaaac tggcgattaa
    12481 cgaattcgtc gcttacctga gtttctcccc atacctgcaa acgggcggca cgctggaagt
    12541 gaaaaccatt gcgattatct cctttgcgct ttgtggtttt gctaactttg gttctatcgg
    12601 tgttgtcgtt ggggcatttt cggctatttc gccaaaacgt gctccggaaa tcgcccagct
    12661 tggtttacgg gcgctggcag cagcaacgct ttccaacctg atgagcgcga cattgccggg
    12721 ttctttattg gtttagcttg agtttcattg ccggatgtac tcgcttatta agcgttgcat
    12781 ccggcaatcg cgctattgca aataattttt actgcatcat cccggagaat ttattctccg
    12841 ggtccatctc cagcgccaac cctgacaact gctttctgtt tttaatgaga tatcctttct
    12901 tgcttttaat taataaaccg tcgtgaatga attgcgcgag aacatataaa agatgtcgat
    12961 aagaaacgcc taaatactct gccgcttgcg tatgcttttc gtgataaaga tcaccttctt
    13021 gtgagagtaa tataaatgct gccaggcgat tcactaacgg aaatgattga ttctgagtta
    13081 aagaaacaat attacgataa tttttatgac ttaaggtgac gcaaagtttt cgtaaaaata
    13141 gggtgtcgtt taataacagc ggacggtaat gtttcatagg gagcgcaagg caccaacact
    13201 cttcaatcgc ctgtactgca cgcggttcat ggtctttatc gattaactca atctcgccaa
    13261 taaaacaggg ggcggcaaag aaatcgatca gcgacacgcg accattagct agcgtggcgt
    13321 aaagcctggc gcggcctcgc gtcaggtaat acagccagga cggctgttgc ccttcctgca
    13381 caatgtagtc acgcgctaaa aaatgaaaca gccgcgtatc ggctgaaaca tccgttaaaa
    13441 agcaatcctt aaacgcggat tcactcatca attgctgctt aagatcatta ttgtggattt
    13501 ctttcacgat gtttatccgc tatgagattt ctcatattta gcatacgtac gctgttgtta
    13561 agattcaatc atccgcccga tcgaaatttc tttgctgtag aaaaaataac gcaactggaa
    13621 acagaggaaa taaacaatgg aaaagagaaa aattattctg gattgtgatc cgggtcatga
    13681 tgatgctatt gcaatgatga tggcagcgaa acatccggca atagatttat taggcatcac
    13741 tattgtagcg ggtaatcaga cgcttgataa aacattaatt aatggcctga atgtttgcca
    13801 gaaactggag attaatgttc cggtttatgc ggggatgccg cagcccatta tgcgtcaaca
    13861 aatcgttgcc gataatattc acggtgaaac cggactggat ggcccggtat tcgagccgct
    13921 gacccgccag gcagaaagca ctcatgcggt gaaatacatc attgataccc tgatggcaag
    13981 cgatggcgat atcactctgg tgccggttgg tccgctttca aatatcgcgg tggcaatgcg
    14041 tatgcaaccc gcgatcctgc ccaaaatccg tgaaattgtg ctaatgggcg gcgcttacgg
    14101 tacaggcaac ttcacgccat ctgccgagtt caacatcttt gctgacccgg aagccgcacg
    14161 cgtggtgttc acctctggcg ttccattagt gatgatgggc ctcgatctca ccaaccagac
    14221 cgtttgcacc ccggacgtga ttgcacgaat ggaaagggca ggcggccctg ccggagagct
    14281 gttcagcgac atcatgaact tcactctcaa aacccagttc gaaaactacg gcctggctgg
    14341 cggcccggtg cacgacgcca cctgcatcgg ttatctgatt aaccctgatc gcattaaaac
    14401 ccaggagatg tacgtcgaag tggacgtcaa cagtggccct tgctatgggc gtaccgtctg
    14461 cgacgagctg ggcgttcttg gcaagcccgc taataccaaa gtcggcatca ctattgatac
    14521 cgactggttc tggggcttag tcgaagagtg cgtgcgcggc tacatcaaaa cccattaagt
    14581 gttgataaca agccgggtca gtatcgcaag atgcaggccc ggctatttct gaggggcaga
    14641 aagccgtcgc ccaatatgac atgaagagat aataactatg gatgtcatga gaagtgttct
    14701 gggaatggtg gtattgctga cgattgcgtt tttactgtca gtaaacaaga agaagatcag
    14761 cctgcgtacc gttggcgcgg cgttagtgtt acaggttgtg attggcggga ttatgctttg
    14821 gttaccgcca gggcgttggg tcgctgaaaa agtcgctttt ggcgtgcata aagtgatggc
    14881 gtacagcgat gcaggtagcg catttatctt cggttcgctg gtcgggccga aaatggacac
    14941 gctgtttgat ggcgcaggat ttatctttgg tttcagagtg ttaccggcaa ttatcttcgt
    15001 caccgcgctg gtgagtattc tctattacat cggtgtgatg gggatcttaa ttcgcattct
    15061 cggcggtata ttccagaaag cattaaatat cagcaagatc gagtcattcg tcgcggtcac
    15121 taccattttc ctcgggcaaa acgaaattcc ggcaatcgtc aaacccttta tcgatcggct
    15181 gaatcgcaat gaattattta cagcgatttg tagtggcatg gcctcgattg ctggttcgac
    15241 aatgattggt tacgccgcac tgggcgtacc tgtggaatat ttgctggcgg catcgttaat
    15301 ggcgatccca ggcggaatct tgtttgcccg cctgttaagc ccggctacgg aatcttcgca
    15361 ggtttctttt aataacctct ctttcaccga aacaccgcca aaaagcatta ttgaagccgc
    15421 tgcgacaggg gcaatgaccg ggctgaaaat cgccgcaggt gtggcgacag tggtgatggc
    15481 atttgtcgcc atcattgcgt taattaacgg tattatcggc ggcgttggcg gctggtttgg
    15541 ttttgaacat gcctcgctgg agtccatttt aggttacctg ttggctccac tggcgtgggt
    15601 gatgggtgtt gactggagtg atgcgaatct tgccgggagt ttgattggac agaaactggc
    15661 aataaatgaa tttgtcgctt atctcaattt ctcaccctat ctgcaaacgg ctggcactct
    15721 ggatgctaaa accgtggcga ttatttcctt cgcgttgtgc ggtttcgcta actttggttc
    15781 tatcggggtg gtggtggggg cgttttctgc ggttgcgcca caccgtgcgc cggaaatcgc
    15841 ccagcttggt ttacgcgcgc tggcggcggc gacactttct aacctgatga gtgccaccat
    15901 tgccgggttc tttattggtt tagcgtagtg tgcgatttat gagccggata atatgcggtc
    15961 aagcgtcgaa tccggcaaca ttgttacacc attggcacta atgacagcgc gttatcggca
    16021 gacagggtag ggttgtttaa gctgccgctg gcgcgtgaaa ttgccgcgca ggccatcgca
    16081 aaacgggcgc tgtcacggaa attgtttcct tccagaaagc tatataccag gcccgccata
    16141 aaaccatcgt cagcaccaaa actgtctact gtcgtatgcg ctggtgcggt cagcaaaaat
    16201 tgttctccat ccttttcgct gcaataaact gactcatcgg gcaaataaac aaacagttgc
    16261 tgaacacctt gctgatgcaa cgcattcact gcagcattac ggtcagcatc gctggtgatc
    16321 gcctgtcccc ataaaatttc cagctccggt aaagtgggtt tcagggtgtg aatatgcgcc
    16381 agccagtgtt tgattttgcc cgctttgaat tctgaaacgg tatcgacaaa caccgggatc
    16441 tcatcagcga gggtaaagac ccattccagc gcctcggctg tcaggttgca atcagccagt
    16501 accacgcccg catgacgaag taaatcgcgc gacccgttca ataattgcgg cgataactgt
    16561 tccagcagat gggtatcgtt aatcgccagc acggtttcat cgtctcgatt ggcaattgcc
    16621 agatacgtcg atgtgctttg accatgtaaa cgaacgcagc cggagacatt cacgccggcg
    16681 cggcgcgttt cttccaggag catttcgcca taaaagtcat cgccaatcac tgaaagcaaa
    16741 tggacgtcgc ggcctaacag cgccagattg tgggcgatgt tgcgtcccac gccgcccgct
    16801 gagcaatgaa ttgtaccggg atgagaagcc gattgcgggt aacggatatc cgccatcccg
    16861 cgaatatcca tattgattgc ccccaccact acgcagtatt cctgctcggt gagaatgtaa
    16921 cctttgcctt taatccggcc tttgcgcatt aaatccataa tatgcgccgc aacgcgcgaa
    16981 cggctgattt gcagcatgtc cgcaatttcg ttctgctgaa tcagcgggtt acgccgtaaa
    17041 attgcaagga tctccttttc ccggttattc atcttcaggc taccgctttt tcagtttgtt
    17101 gcgctttcag ccaggcgatc tcttctgccc agatatccgg gttgatggtt tcgaggatca
    17161 gcggaatgcc gtcgaaacgg tcgtcctgca tgatccagcg gaacgcatca tgaccgatat
    17221 tgccttcacc gaggctatga tggcggtcaa cgcggctgcc aaaggtgctt ttcgcatcgt
    17281 taaggtgcat cccgcgcaga tacttaaagc cgacaatacg ggcaaaatca gcgaacgttt
    17341 tctcgcattc ggctggagta cgcaaatcat acccggcagc gaaagcatgg caggtatcaa
    17401 tgcagacgcc gacacgggat ttatcttcca cgccgtcgat aatcgccgcg agatgttcga
    17461 atttaaaccc taagttactg ccctgaccgg cggtgttttc aatcaccgcg gtcacacctt
    17521 gagttttatc cagcgcaatg ttgatggatt cggcaatacg cgcaaggcaa tcctcttctg
    17581 aaatctgcat cagatggctg ccagggtgga agttgagcaa agaaagcccc agctgttcgc
    17641 aacgctgcat ttcatctata aaggcatcgc gcgatttttc cagagcttca gtgaccggat
    17701 gtccgaggtt aatcagatag ctgtcgtggg gaagaatttg cgccgatgtg tagtgatatt
    17761 tttcacaggc ggctttgaat tcatcgatgg tttgcgtcgt gagcggtgcg gcacgccact
    17821 gacgttggtt tttggtgaac aaggcaaacg cggttgcgtc gatttcggcg gcgcgaattg
    17881 cggcatttgc cagaccgcca gcagcactaa cgtgcgctcc aatgtatttc atgcgaggac
    17941 tcctgttaaa cccgctggag gaaaacggta atgatagcgg gttaacagga gcaagatgta
    18001 gtggtttatg cgatgacgct ttgaatcaca tagttaatcg caccaccacc aacaatcagc
    18061 caggcaaaca gtaccagtgc catcagcaga ggtttcgccc cggctttttt cagcgcgctg
    18121 acgtgagtgg tcagacccag cgccgccatc gccattgcca gcaggaaggt atccagcgtt
    18181 accagcatgt tcaccacgct ctgcggtaac aggtggaacg agttaaagat ggcaactacg
    18241 atgaacaaga tggcaaacca cggaatagtt attttgcttt tctcgccgct gttcgcccca
    18301 gacagctgtt taacacgcgc cgccagcagg atgaggaacg gagccagcat catcacgcgc
    18361 agcattttgg aaataactgc tgcgttttcc gcatctgggc tgatggcatg acctgccgcc
    18421 accacctgcg ccacttcgtg cacagtagaa ccaatgtaga taccgaaagt ttccggacta
    18481 aaccattgag acatcagcgg atatatcgcc gggtagagga aaatcgcgac ggtcccgaag
    18541 ataacaacgg ttgcaacagc cacggttact ttactggctt ccgctttcac taccggctca
    18601 gtcgccagta ccgcggcagc accacagata ctgctaccgg caccgatcaa ccagctggtg
    18661 tgcttatcca gaccaaacac tttctgcccc aggaagcaag ccagcaggaa ggtactggac
    18721 agcgtcaaca cgtcaatgat gatcccactg ataccgacat cggcaatttg cgagaacgtc
    18781 agacggaagc cataaagaat gatacccaga cgtaataaat attgcttggc aaacagcaca
    18841 ccaccgtcac agcttttcca gatgtgcgga tagatggtgt tgcctaaaac catccccaac
    18901 aagattgcga gggtgagggc actaaacccg gcacccgcaa ccgcgggaat ggaaccaccc
    18961 cacagggcga ccccggtgat aactgcactc agggctaacc ccggaataaa atgccacagt
    19021 gtacgatgtt gtttctgtaa ggtgatattc gtcataaccc tctcctttaa ccgggcataa
    19081 ggttacgact aactggttta aaaataaaat tgattatata tttataatta atctttataa
    19141 gtggtaagcg actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa
    19201 aagtggatca accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc
    19261 agccttgacc gacctggaag ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag
    19321 actggttgtt aatgaacacg ggcggctgct ctatccgcgt gcgttggcat tgcttgaaca
    19381 ggcggttgaa atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag
    19441 tagtaccatc ggtaactaca ttctgcctgc agttatcgcc cgttatcgcc atgattatcc
    19501 gcagttgccg attgaactta gcgttgggaa tagccaggac gtgatgcaag cggtgctgga
    19561 tttccgcgtt gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga
    19621 accgtggctg gaagacgagc tggtggtttt cgccgcaccg acttcgccgt tggcccgtgg
    19681 cccggtcacc ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg
    19741 cacgcgggag attgtcgatt atctgttgct gtcacattta ccgaagtttg agatggcgat
    19801 ggaattaggt aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg
    19861 cctgtcgcga cgtgtgattg aagatcaatt gcaggcaggc acattaagtg aagttgcggt
    19921 ccctctgccg cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc
    19981 caacgcgcta cggcgctttc tggactattg cgatcccgcg aatgtaccgc gttaagttgc
    20041 tgtacaagaa catgctggtg ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc
    20101 gccagaaaag agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat
    20161 aaccgtgtat ttctgccgga aggattgcca atcgtctgct acaatcgcgc ctcattttta
    20221 agatggatag catttttgta tggtttccga aactaaaacc acagaagcgc cgggcttacg
    20281 ccgtgaatta aaggcgcgtc acctgacgat gattgccatt ggtggttcca tcggtacagg
    20341 tctttttgtt gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc
    20401 gtatatgttg attggcctga tggtttactt cctgatgacc agtctcggtg aactggctgc
    20461 atatatgccg gtttccggtt cgtttgccac ttatggtcag aactatgttg aagaaggctt
    20521 tggcttcgcg ctgggctgga actactggta caactgggcg gtgactatcg ccgttgacct
    20581 ggttgcagct cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag
    20641 tgcgttgttc ctcggtgtta tcttcctgct gaactacatc tcagttcgcg gctttggtga
    20701 agcggaatac tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg
    20761 cgtgctgatg attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac
    20821 aatcggcgaa gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt
    20881 cggcttctct ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc
    20941 ggcgaaaaac attccacgtg cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt
    21001 gttcgcgatc ctgattatca gcctgattat tccgtacacc gatccgagcc tgctgcgtaa
    21061 cgatgttaaa gacatcagcg tcagtccgtt caccctggtg ttccagcatg cgggtctgct
    21121 ctctgcggcg gcggtgatga acgcagttat tctgacggcg gtgctgtcag cgggtaactc
    21181 cggtatgtat gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg
    21241 cattttcgct aaactgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt
    21301 gattgccggt ctgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct
    21361 gctgaatacc tccgggatga ccggttttat cgcctggctg gggattgcca ttagccacta
    21421 tcgcttccgt cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc
    21481 aggtttcttc ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg
    21541 ccagaactac gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta
    21601 tattggtatc ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca
    21661 cttcgtacgc tacagcgaaa tgaagttccc gcagaacgat aagaaataag tttcctccct
    21721 tccttgctaa gccctctcaa ccgagagggc tttttcaatt tcatttccct gacaaatcat
    21781 gcggatataa aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgcc
    21841 gcaatcaaaa aaggctgaca aatcagaggc tggtccggct tgctgggatg atcacctgca
    21901 tacaaataag tccaccgcga tgctgccgta cgcaagggga cgtgaagaag atgtgagcga
    21961 taacccattt tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt
    22021 tcgtacgggt cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg
    22081 atgatgatgg cgaaacgatg gttgtcactg catcttccgt tgaacaaaac cttaaagatg
    22141 cacccgccag tatcagcgtc attacccagg aagacctgca gcgaaaaccg gtacagaatc
    22201 tgaaggatgt cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta
    22261 agggcgttag tattcgtggt cttgacagca gctacaccct gattctcgtc gacggtaaac
    22321 gcgttaactc ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg
    22381 tcgattccat cgaacgtatt gaagtggtcc gtggcccgat gtcgtcgctg tacggttccg
    22441 atgcgctcgg cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta
    22501 ccgttaccgt cgataccacc attcaggaac atcgcgatcg cggtgatacc tataacggtc
    22561 agttctttac cagtggacca ttaattgatg gtgtgctggg aatgaaagct tacggcagcc
    22621 tggcaaaacg tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc
    22681 cgcgtattga aggattctcc agccgcgacg gcaatatcga atttgcctgg acaccgaatc
    22741 aaaatcacga ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgactcgt
    22801 tggacaaaaa ccgcctggaa cgccagaact actccgtcag ccataatggg cgttgggatt
    22861 acggcaccag cgaactgaaa tactacggtg agaaagtcga gaacaaaaac cctggcaaca
    22921 gcagcccgat aacttccgaa agcaatacgg tcgacggcaa atacaccttg ccgctgacgg
    22981 cgattaatca gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg
    23041 tgaacctgac cgggggaacc agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg
    23101 aagatgaatg gcggatcttc gagccgctgg cgctgacgac cggcgtacgt atggacgatc
    23161 atgaaaccta cggtgaacac tggagtccgc gtgcctacct ggtttataac gccaccgaca
    23221 ccgtaacggt gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta
    23281 gccctgactg gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc
    23341 tgaaaccaga aaccagcgaa agttgggagc tggggctgta ctacatgggt gaagaaggct
    23401 ggctggaagg ggttgaatcc agcgttaccg ttttccgtaa cgatgtgaaa gatcgcatca
    23461 gcatcagccg tacgtctgac gtcaatgctg caccgggcta ccaaaacttt gtcggttttg
    23521 agacgggcgc taacggacgg cgcataccgg tatttagcta ctacaacgtt aacaaagctc
    23581 gtattcaggg cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcac
    23641 tcaactacac ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg
    23701 atctgccgtt ccataccgct aacggtacgc tggactggaa accgctggcg ctggaagact
    23761 ggtcattcta tgtttctggt cactataccg ggcagaaacg agccgacagc gcgacggcta
    23821 aaacaccggg cggttatacc atctggaata ccggcgcggc ctggcaggtg actaaagacg
    23881 tcaaactgcg cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt gacgactaca
    23941 gctataacga agacggacgt cgttacttta tggcagtgga ttatcgcttc tgatgagaag
    24001 atgcccggcg aaccgggcgg actttcactt cagtaaatac tgcgcatgga agcgcaggtg
    24061 atcctctata aaagaggcga tgaagtagta actgtgatca tatcccggct gaatacgcag
    24121 cgtcatcggc catgctttct ggcgcgcggc ttctgcaagt actgcaggtt gcagttggtc
    24181 agcaagaaac tgatcgttat caccctgatc gataagcgtc gggatcgcat cctgcgcgtt
    24241 actggcatac atcagcgcgc aactgtccca ttccagccat gcatttttgt cctcacctaa
    24301 atagcggcta aacgctttga ttccccacgg gacgctgcac ggattcacaa ttggcgcaaa
    24361 ggcggaaacg ctggtgtatt tgcccgggtt tttcagcgcc ataatcagcg caccgtgacc
    24421 acccattgaa tgaccgctaa tagcgcagcg gtcgctgaca ttaaattgcg actgaaccag
    24481 cgccggtaat tcatcgcgca gataatcata catccggtaa tgcgtcgccc acggcggttg
    24541 cgtggcatta agataaaagc ctgcgccctg gcctaaatcg tagccatcgt cgttggcaac
    24601 ctgttcaccg cgcgggctgg tgtctggcat taccagcaca atccccagtt ccgccgctac
    24661 ccgctgagca cccgccttgg tggtgaagtt ctcgtgattg caggttaatc cggaaagcca
    24721 gtacagcact ggtggcgggg tgtgatcacg agggggtggg agaaagatac tgaacgtcat
    24781 cgggcagttt aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg
    24841 gtgctcttcg agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca
    24901 taccgtttat tcaatgtgct gtgagtaact ttcacttccg tatttgcata acgatgtttt
    24961 aacatctgct gatgaaaggc agcggcaatt acaataatta tcgctgtgaa tactggatta
    25021 tgtgcgccgc ctcacgcaca ataatcaggc tgtaaatcag cttaataact ttgcccccac
    25081 gcagggcgga ggcgtcacac ctgcaggaga aatcataaat gccatcactc agtaaagaag
    25141 cggccctggt tcatgaagcg ttagttgcgc gaggactgga aacaccgctg cgcccgcccg
    25201 tgcatgaaat ggataacgaa acgcgcaaaa gccttattgc tggtcatatg accgaaatca
    25261 tgcagctgct gaatctcgac ctggctgatg acagtttgat ggaaacgccg catcgcatcg
    25321 ctaaaatgta tgtcgatgaa attttctccg gtctggatta cgccaacttc ccgaaaatca
    25381 ccctcattga aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat atcactctga
    25441 ccagtacctg tgaacaccat tttgttacca tcgatggcaa agcgacggtg gcttatatcc
    25501 cgaaagattc ggtgatcggt ctgtcaaaaa ttaaccgtat cgtgcaattc tttgcccagc
    25561 gtccgcaggt acaggaacgt ctgacgcagc aaattctcat tgcgctacaa acgctgctgg
    25621 gcaccaataa cgtggctgtc tctatcgacg cggtgcatta ttgcgtgaag gcgcgtggca
    25681 tccgcgatgc aaccagtgcc acgacaacga cctctcttgg tggattgttc aaatccagtc
    25741 agaatacgcg ccacgagttt ctgcgcgctg tgcgtcatca caactgatta aaaggcagga
    25801 accatggagc gcaacgtcac gctcgatttt gttcgcggcg tcgccattct ggggatcctg
    25861 ctattaaaca tcagcgcctt tgggctacca aaggcggctt atcttaatcc cgcctggtac
    25921 ggcgctatta cgccgcagga tgcatggacc tgggcatttc tcgatctcat cggccaggtg
    25981 aaattcctca cgctttttgc gctgctgttt ggtgctggcc tgcaaatgtt gctgccccgt
    26041 ggcagacgct ggatccagtc gcggttaacg ctgttagtct tgctgggctt tattcacggt
    26101 ttattgttct gggacggcga tattctgctg gcttacgggc tggtgggctt aatctgctgg
    26161 cggctggtgc gcgatgcgcc atcggtaaaa agcctgttta atacaggcgt catgctttat
    26221 ctggtggggc ttggcgtttt gctgttattg gggctgattt ccgacagcga gaccagccgc
    26281 gcctggacgc cggatgcatc ggctattttg tatgaaaaat actggaagct tcacggcggc
    26341 gttgaagcga tcagtaatcg tgccgatggt gttgggaaca gtttattggc actgggcgca
    26401 cagtatggct ggcaactggc tgggatgatg ctcattggtg ccgcattgat gcgcagtggc
    26461 tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt gctggtggcg
    26521 attggggtga tcattaacct tcctgccatc gccctgcaat ggcggctgga ctgggcatat
    26581 cgctggtgcg ccttcttact tcagatgccg cgggaactga gtgcaccgtt tcaggcgatt
    26641 ggctatgcgt cgctgtttta tggtttctgg ccgcaattga gccgctttaa gctggtgctg
    26701 gcgatcgcct gcgtcggacg gatggcgctg accaactatc tattgcaaac gctgatttgt
    26761 accacgcttt tttatcacct cggtttgttt atgcagtttg accgtctgga attgctggcg
    26821 tttgttattc cggtatggct ggcgaatatt ctcttctctg ttatctggct gcgttacttc
    26881 cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgctgc cggaccggca
    26941 atatctaaaa catcaagata acgatctgga tcacattcgt taacaaaacg gctgtaaccg
    27001 tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa aatagccgcc
    27061 agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac cattcgtgat
    27121 gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gggtgctcaa taacagcacg
    27181 ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg tgagtgagct ggattatcgg
    27241 ccaaacgcca atgcccaggc gctggcaact caggttagcg acaccattgg cgtggtggtg
    27301 atggacgttt ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct ggtcgctcag
    27361 cagcatcaga aatacgtgct aatcggcaat agctatcatg aagcggaaaa agagcgtcac
    27421 gccattgagg tgttaattcg ccagcgttgt aatgcgttga ttgttcactc aaaagcattg
    27481 agtgacgatg aactggcgca atttatggat aacattcccg gtatggtgtt aatcaaccgc
    27541 attgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg tgccagaatg
    27601 gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc ttccagccac
    27661 ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa agagcaggat
    27721 attattccgc cggaaagctg gattggcact ggtacgccgg acatgccggg cggtgaggcg
    27781 gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc ttataacgac
    27841 aatatggctg ctggcgcact gacagcatta aaagataatg gcattgcgat tccgttacat
    27901 ctctcaatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc gcaattaacg
    27961 accgtgcgtt atcccattgc ttcaatggcg aaattagcca ccgaactggc cttgcagggg
    28021 gcagcaggca atattgatcc tcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc
    28081 cattctgtcg caacacgcca gaatgcggcg gcgatcacta actcaacaaa tcaggcgatg
    28141 taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgtgata gctatgattg
    28201 caccgtttta acgttgtaac ccgtatgtaa cagtgaataa tcactttcac cgaggtaata
    28261 gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga agaacgaatt
    28321 ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc cgccgagcat
    28381 ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa gaaggtgtta
    28441 accctgtctg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc tgctgaaact
    28501 cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt gcgcaaggct
    28561 attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga ttctcagaat
    28621 gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt gaaggcactg
    28681 gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa
    28741 aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga tagctacgac
    28801 aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaggg cgatttgatt
    28861 gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca gattcagttc
    28921 gtactgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac ttacgtgatt
    28981 aaagaattga acgataaagg catcaaaact gaacagttac agttagatac cgctatgtgg
    29041 gataccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa cgccaacaaa
    29101 atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga agcactgaaa
    29161 gcacacaaca agtccagcat tccggtgttt ggcgtcgatg ctctgccaga agcgctggcg
    29221 ctggtgaaat ccggtgcact ggcgggcacc gtactgaacg atgctaacaa ccaggcgaaa
    29281 gcgacctttg atctggcgaa aaacttggcc gatggtaaag gtgcggctga tggcaccaac
    29341 tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa agacaacctg
    29401 gctgaattta gcaagaaata agactgattg ggcgcagtct attactgcgc ccttattgta
    29461 tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg ggaatacttg
    29521 ttggaaatga gcggtatcaa caagtctttt cctggtgtta aggcacttga taacgttaat
    29581 ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggcgc aggaaaatca
    29641 acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat tttattccag
    29701 ggtaaagaga tcgatttcca ttcggccaaa gaagcactgg aaaatggtat ttcgatggta
    29761 caccaggagt taaacctggt attacaacgt tcggtgatgg acaacatgtg gcttgggcga
    29821 taccccacca aaggcatgtt tgtcgaccag gacaaaatgt accgcgaaac caaagcgatt
    29881 tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt atctgtttcg
    29941 caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat tgtgattatg
    30001 gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac tattattcgt
    30061 aaattaaaag agcgcggctg cggtattgtt tatatctcgc ataaaatgga agaaatcttc
    30121 cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac cgaaccgctg
    30181 gcagggctga cgatggacaa gattatcgcc atgatggttg ggcgttctct taaccagcgt
    30241 tttcctgaca aagaaaacaa gccgggcgaa gtcatcctcg aggtgcgtaa cctgacgtca
    30301 ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga gatcctcggt
    30361 attgccggtc tggtgggggc gaaacgtacc gatattgttg agacgttatt tggtattcgc
    30421 gagaaatcgg ctggcacaat tacgttgcac ggcaaaaaga tcaataacca taatgccaac
    30481 gaagccataa accacggatt tgcactggta actgaggagc gccgctcaac gggaatttat
    30541 gcctatctgg atatcggttt taactcgtta atttccaata ttcgcaacta caaaaataaa
    30601 gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat tgattcgatg
    30661 cgggtaaaaa cgccgggtca tcggacgcaa attggttcgc tctccggtgg taatcagcaa
    30721 aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct cgatgaaccg
    30781 acgcgcggta ttgatgtcgg ggcgaagttt gagatctatc agttaattgc cgaactggcg
    30841 aagaaaggca aggggattat tattatctcc tctgaaatgc ctgagttgtt agggataaca
    30901 gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac aaaaacaaca
    30961 acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg gcttcccatg
    31021 agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat ttacgtcgtt
    31081 cttttagttt tgctggcgat tattattttc caggacccaa catttttaag tctgttgaac
    31141 ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg cgtggcaggg
    31201 ttaattgtca cccaggggac cgacctttct gcaggtcgcc aggtggggct ggcggcagta
    31261 gtagcggcga cgttattgca gtccatggat aacgccaaca aagtgttccc ggaaatggcg
    31321 acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt gatcggtttg
    31381 atcaacggtc tgattatcgc ttatctcaac gtgacgccgt tcattaccac gctcggcacg
    31441 atgatcatcg tctatggcat caactcgctc tattacgact ttgtcggggc gtcgccaatt
    31501 tctggttttg acagtggctt ctctaccttt gctcagggct ttgtcgcgct ggggagtttc
    31561 cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg
    31621 aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga agcggcaaaa
    31681 gtatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc tggcgtgttc
    31741 tatgcctttg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa caacctcggc
    31801 tttatgtatg aactggatgc tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc
    31861 ggtgtgggga cggtgattgg cgtggtgacc ggggtaatta tttttaccgt catcaactat
    31921 ggcctgacgt atatcggcgt aaacccatac tggcagtaca tcatcaaagg ggcgattatt
    31981 atcttcgccg tagcgctgga ttcactgaag tacgcgcgta agaagtgaaa tgattgtggt
    32041 ccgcattcgg tcataaacgc acataacatc atgatctgtg tggttttgta ggccggataa
    32101 ggcgttcacg ccgcatccgg cgacaggagc caaatgccgg atgcaacgct ggcgcgtttt
    32161 atccggccta caatgagctc aacatactga tttgtatggt tttgtaggcc ggataaggcg
    32221 ttcaagccgc atccggcgac aggaaccaaa tgccggatgc gtctctggtt ttacaacgtt
    32281 accgggtgtt ctttctcgcc tttcttaaac ttcacttccc cgagctcaat acaacccacc
    32341 gggcagacgt gaccacaaag cagacaaccc acacatttct cggtattaca atgcggtgtg
    32401 cgggttttct cgctccattc catcgcctgg tgaccgccgt cgtaacagga aatataacag
    32461 cgtccacagc caacacattt atcaagattg atacggggat agacaatata actgcggtct
    32521 aaatcttccg ccgggacaat attgttattc gccagaccta ccatctcctg caacgaatca
    32581 aatccctgat cggcgagata atgcgacaac ccgctcgcca tatcttctac gatgcgatag
    32641 ccgtactgca taatgccggt ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc
    32701 gccgcatcca cccaggtttc aatgccgccg ataccgctga ttgggaaatc gcgcagttct
    32761 ggatgggtgc gcatttgctg aatgaagcgc agggcgatcg gttttaccgc tttaccggaa
    32821 tatccggaaa tactcgattt tccgttaacg atcggcatac cgattttctg attaagatcg
    32881 atattggtga tggatttaac ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc
    32941 gccgccagcg ccacttcgca catatcaccg atattcggcg tcatcttcgc cagcattggc
    33001 agcgtggaac cccgtttcac tgctcgacaa tatttttcta ccagctccgg gctttgcccg
    33061 acatcgctac ccatcgcatg agaagtcatt tgcggacagg agaagttaca ctcgatcata
    33121 tccgcgccag cttcttgcac caggcgcgcc agctcctccc attgctgctc attttccccc
    33181 atgatcgaag cgatcaatac tttgtccggg taatcttcct tcagccgacg cagggcggcc
    33241 agattctctt ccaacggatg ttcagcaatc tgctccatat ttttgaagcc gataaaaccg
    33301 gtatcttctt tcaccagatg atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg
    33361 atcgttttaa acaccacgcc gccccaacct gtgtcgtagg ctttggcaca catctcatag
    33421 cagttgccta ccggcgaaga agagaggcag aacgggttgg gaaacttcac gccgcaaaaa
    33481 gtaatcgaaa gatctttcgt taacatgagc aagctccctc taaatagtga tgaatcgcct
    33541 cggcggcttc tttcccggtt ttcacggcat agaccacggt tttgtcaccc tcaacaatat
    33601 cgccagcagc aaagacttgc ggatcgcggg tctgataatt ttgtgttttg atggtgttac
    33661 gctgcggctc taacttcgca aaggcatcca gtctggcatg ctgaccgacg gcgagaataa
    33721 ttttatctgc caccatcgtc agttcgcccg ataaccgtac atgcttaaac gtgactttat
    33781 tgccttccac ggctactggc gtgaatccat cgatgatcga aacgcccagc tcccgggcgc
    33841 tggtaaactc tttttcgctt gccggaaact catctaactc ttcacgcgct acgcaagtta
    33901 ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc catcgcgaca tcaccgccgc
    33961 cgataattaa tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt gcacgttgca
    34021 agaagtcgac ggcaatctca acgtcactat gctcaaacag cggtagcccg gaaccgcttg
    34081 ataaccccac ggtgaccagt accgcgcggt tttctgcttt cagctgctca agggtgagtg
    34141 tgttaccgac ttcgttgttg cacttaatgg tcacgcccat tttttcaata cgggcgatct
    34201 ctgcatccag cactgattgc ggtaaccgga attgcggaat accgttacgc aaccagccac
    34261 cggggtgcgc ttctttctca taaatcgtga cgtcataacc ctggtttgtc agtgtcacac
    34321 tggcctgcaa tccggcagga cctgcgccaa taatcgcgac tttgccgagc gttttagtac
    34381 cgggctgata aatttccatt ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc
    34441 ggccgatatc aatgggagca tcaacaccgg cacgggtaca accgctttgg cataatttct
    34501 ccgtcgggca cactctggca caaacggcac cgagggcatt attttcgcga attgtctcgg
    34561 cagcgccttt aaaattacga aagtagattg agcgaataaa tttccccgga tcggtctgtg
    34621 ccgggcaagc ctgactacag ggagcgtcgt gacataataa acagcgagag gcttctttaa
    34681 tcgccagtaa agacgtaaaa gccggagtga gttcatccag ataattttgt tgcggcatgt
    34741 gatcttcctt aatgctatag gtaattaagg cgagcgttta gtaaataata atgtactgat
    34801 attaaggcta agcgtgcggg ggaatttaaa ctgtgaaaag attcacaaat agctgcggtt
    34861 aggaatgtga tatccgcagc ttatcaggtg aagtaataat tattctcaat aattaactgg
    34921 agaggggaat attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg
    34981 aagccgatat atgccaccat ataagcgaaa aagagtccgg gattttttgc tcgccagaca
    35041 tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc
    35101 catgcttcgc ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc
    35161 tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga
    35221 cggcgggata cccctgcgca tcttccggta cctggtgcat agccggaata gggaccagcg
    35281 gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg
    35341 cggcaaattc acgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact
    35401 gagcctgaat ccccatatgc agcgcaataa ataatgctcg ctcacagtgg aaacgttggg
    35461 tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg gaatccagcg
    35521 tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca gcgattaaat
    35581 ctttgcgcat ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata
    35641 gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat
    35701 actgattaat tacgccagta cgataatatt ttgctgttcc gagcaccaca ccgacctggc
    35761 ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc
    35821 gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca
    35881 cgcgctttaa catgcaggag actcgatcta aggaaaattt ccatcaggct actttacccg
    35941 ctggttttgc gcaagaaacg gcaaactgtt tgccgggatt ttcgacacca ggagagggct
    36001 ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagcactcg
    36061 gtcgatactg tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa
    36121 cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt
    36181 gagattcaac agaattaacg ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt
    36241 ttcagcgtag ctgccactga aaatacgacc gtctttacat tccagcgcga caccgcttgg
    36301 cgacttactg taaggcatgt gcgaacggtt tgccgccgca atcgctgcct gagaaagcgc
    36361 atcacccgtc agcgcatagc cgtgatcctg ttcgtccatc agcagcgttt taatctccag
    36421 atctttcggc ccaaaggcat ctggcagata gtcacgcagc gcgtgtgcct cgcggcccgg
    36481 cagatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc
    36541 acaaggcgtg tagttaacgg tgatggctgc aagcgctttt tcaccactca accaggcgtg
    36601 gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gtcgcaccaa taaattccat
    36661 attggcaccg aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt
    36721 cgacaatggc gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc
    36781 ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg
    36841 gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc
    36901 aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca
    36961 gtttacgaac ccgttgtggc tttatatgtg atctgaatct cattaattat gcaacgcgtg
    37021 aaacatacat caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat
    37081 cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca gcgctagcga
    37141 actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga
    37201 ggcagtcccc attgccagac cgcgcgcagc tttggtacga atacgcatcg cattaagcaa
    37261 tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc
    37321 cggaataccg ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg
    37381 caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt
    37441 caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg
    37501 gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg gttgcagcag
    37561 atcgtttagc acttcgctac ctttaaagta gctgtcgtaa gagatgccag tcagcattaa
    37621 aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaagggaa acttataccg
    37681 tgccgccagt ttgcgggcgg caaaaaagac aatcaatgtc agcggtaatg accaccagat
    37741 attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc gtgtaccagt
    37801 tgcgaactcc agctcaccac cagaaaaacc accagcgtac tgactgcaca ggaaaccact
    37861 accgggccaa actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc
    37921 acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac ccattttgcc
    37981 ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga
    38041 atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg
    38101 acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca
    38161 gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa
    38221 tcagcgctta ttttttcgct ccccggcatt acgcaatgcg cagcgaactc aggcatacat
    38281 caagcataga gagggattgc agtttgcagc aatccctctt tgcagacctt taatccgctg
    38341 accataggcg ctaacatagc gcctcatttt tttgcgggtg atgatatgcg tgtgttactg
    38401 gcaccgatgg agggagtgct tgactctctg gtgcgtgaat tgctgaccga agttaacgac
    38461 tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc
    38521 tttcatcgca tttgccctga gctacaaaac gccagccgga caccatctgg tacgctggtg
    38581 cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc ccgtgcggtg
    38641 gagttaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac ggttaacggt
    38701 agcggcggcg gggcgacgtt actcaaagat cctgaactca tctaccaggg tgcaaaagcg
    38761 atgcgtgaag ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct gggctgggac
    38821 agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggcgc tacggagctg
    38881 gtggtgcatg ggcggacgaa agagcagggt taccgcgcgg agcatattga ctggcaggcg
    38941 attggcgata ttcgccagcg gctgaatatt ccggtgattg ccaacggtga aatctgggac
    39001 tggcagagcg cgcaacaatg catggcgatc agcggctgcg acgcagtgat gattggtcgc
    39061 ggggcgctca atattcccaa cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg
    39121 tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc
    39181 gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat
    39241 gaagcaacgg aattatttca gcatgttcgg gtgttgaata attcccctga tattgcaagg
    39301 gctattcagg caattgatat cgagaaactc taaaaaacca tagtgatgtt ttttgacaat
    39361 gtgttagctc tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat tgccgaggcg
    39421 ttccctgaag gccggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg
    39481 ttacctcgcg cccgacccgg tagccgtaag ggataagccg agggcaccgc gcagcggcga
    39541 ttttgt
//
LOCUS       DAZUGK010000018        39166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZUGK010000018 DAZUGK010000000
VERSION     DAZUGK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.018"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..1732
                     /gene="aslA"
                     /locus_tag="ACHY5W_000840"
     CDS             77..1732
                     /gene="aslA"
                     /locus_tag="ACHY5W_000840"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFS4445786.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1899..3134)
                     /gene="aslB"
                     /locus_tag="ACHY5W_000841"
     CDS             complement(1899..3134)
                     /gene="aslB"
                     /locus_tag="ACHY5W_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS4445787.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLNASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3281..3357)
                     /locus_tag="ACHY5W_000842"
     tRNA            complement(3281..3357)
                     /locus_tag="ACHY5W_000842"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3321..3323),aa:Pro,seq:tgg)
     gene            complement(3400..3486)
                     /locus_tag="ACHY5W_000843"
     tRNA            complement(3400..3486)
                     /locus_tag="ACHY5W_000843"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3450..3452),aa:Leu,seq:cag)
     gene            complement(3507..3582)
                     /locus_tag="ACHY5W_000844"
     tRNA            complement(3507..3582)
                     /locus_tag="ACHY5W_000844"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3547..3549),aa:His,seq:gtg)
     gene            complement(3641..3717)
                     /locus_tag="ACHY5W_000845"
     tRNA            complement(3641..3717)
                     /locus_tag="ACHY5W_000845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3681..3683),aa:Arg,seq:ccg)
     gene            complement(3820..5205)
                     /gene="yifK"
                     /locus_tag="ACHY5W_000846"
     CDS             complement(3820..5205)
                     /gene="yifK"
                     /locus_tag="ACHY5W_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4445788.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5396..6136)
                     /gene="wecG"
                     /locus_tag="ACHY5W_000847"
     CDS             complement(5396..6136)
                     /gene="wecG"
                     /locus_tag="ACHY5W_000847"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFS4445789.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6139..7491)
                     /gene="wzyE"
                     /locus_tag="ACHY5W_000848"
     CDS             complement(6139..7491)
                     /gene="wzyE"
                     /locus_tag="ACHY5W_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFS4445790.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSLVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGVLFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7488..8567)
                     /gene="rffT"
                     /locus_tag="ACHY5W_000849"
                     /gene_synonym="wecF"
     CDS             complement(7488..8567)
                     /gene="rffT"
                     /locus_tag="ACHY5W_000849"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFS4445791.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8564..9814)
                     /gene="wzxE"
                     /locus_tag="ACHY5W_000850"
     CDS             complement(8564..9814)
                     /gene="wzxE"
                     /locus_tag="ACHY5W_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFS4445792.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9816..10946)
                     /gene="rffA"
                     /locus_tag="ACHY5W_000851"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9816..10946)
                     /gene="rffA"
                     /locus_tag="ACHY5W_000851"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFS4445793.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10951..11625)
                     /gene="rffC"
                     /locus_tag="ACHY5W_000852"
                     /gene_synonym="wecD"
     CDS             complement(10951..11625)
                     /gene="rffC"
                     /locus_tag="ACHY5W_000852"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFS4445794.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11603..12484)
                     /gene="rfbA"
                     /locus_tag="ACHY5W_000853"
     CDS             complement(11603..12484)
                     /gene="rfbA"
                     /locus_tag="ACHY5W_000853"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4445795.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12503..13570)
                     /gene="rffG"
                     /locus_tag="ACHY5W_000854"
     CDS             complement(12503..13570)
                     /gene="rffG"
                     /locus_tag="ACHY5W_000854"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4445796.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFSFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGMPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13570..14832)
                     /gene="wecC"
                     /locus_tag="ACHY5W_000855"
     CDS             complement(13570..14832)
                     /gene="wecC"
                     /locus_tag="ACHY5W_000855"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFS4445797.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14829..15959)
                     /gene="wecB"
                     /locus_tag="ACHY5W_000856"
     CDS             complement(14829..15959)
                     /gene="wecB"
                     /locus_tag="ACHY5W_000856"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFS4445798.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKSILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKIILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16015..17061)
                     /gene="wzzE"
                     /locus_tag="ACHY5W_000857"
     CDS             complement(16015..17061)
                     /gene="wzzE"
                     /locus_tag="ACHY5W_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFS4445799.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17073..18176)
                     /gene="wecA"
                     /locus_tag="ACHY5W_000858"
     CDS             complement(17073..18176)
                     /gene="wecA"
                     /locus_tag="ACHY5W_000858"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFS4445800.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAIQPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18416..19675)
                     /gene="rho"
                     /locus_tag="ACHY5W_000859"
     CDS             complement(18416..19675)
                     /gene="rho"
                     /locus_tag="ACHY5W_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4445801.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19760..19861)
                     /gene="rhoL"
                     /locus_tag="ACHY5W_000860"
     CDS             complement(19760..19861)
                     /gene="rhoL"
                     /locus_tag="ACHY5W_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFS4445802.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20002..20331)
                     /gene="trxA"
                     /locus_tag="ACHY5W_000861"
     CDS             complement(20002..20331)
                     /gene="trxA"
                     /locus_tag="ACHY5W_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFS4445803.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20462..21727
                     /gene="rhlB"
                     /locus_tag="ACHY5W_000862"
     CDS             20462..21727
                     /gene="rhlB"
                     /locus_tag="ACHY5W_000862"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFS4445804.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDTLMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21861..23345
                     /gene="gppA"
                     /locus_tag="ACHY5W_000863"
     CDS             21861..23345
                     /gene="gppA"
                     /locus_tag="ACHY5W_000863"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFS4445805.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPIDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23392..25413)
                     /gene="rep"
                     /locus_tag="ACHY5W_000864"
     CDS             complement(23392..25413)
                     /gene="rep"
                     /locus_tag="ACHY5W_000864"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFS4445806.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25630..26079
                     /locus_tag="ACHY5W_000865"
     CDS             25630..26079
                     /locus_tag="ACHY5W_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4445807.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26278..26559
                     /gene="ppiC"
                     /locus_tag="ACHY5W_000866"
     CDS             26278..26559
                     /gene="ppiC"
                     /locus_tag="ACHY5W_000866"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFS4445808.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26605..28080)
                     /gene="ilvC"
                     /locus_tag="ACHY5W_000867"
     CDS             complement(26605..28080)
                     /gene="ilvC"
                     /locus_tag="ACHY5W_000867"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFS4445809.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28230..29120
                     /gene="ilvY"
                     /locus_tag="ACHY5W_000868"
     CDS             28230..29120
                     /gene="ilvY"
                     /locus_tag="ACHY5W_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFS4445810.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERNDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(29117..30661)
                     /gene="ilvA"
                     /locus_tag="ACHY5W_000869"
     CDS             complement(29117..30661)
                     /gene="ilvA"
                     /locus_tag="ACHY5W_000869"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFS4445811.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVSVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILKMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30664..32514)
                     /gene="ilvD"
                     /locus_tag="ACHY5W_000870"
     CDS             complement(30664..32514)
                     /gene="ilvD"
                     /locus_tag="ACHY5W_000870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFS4445812.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRANLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32579..33508)
                     /gene="ilvE"
                     /locus_tag="ACHY5W_000871"
     CDS             complement(32579..33508)
                     /gene="ilvE"
                     /locus_tag="ACHY5W_000871"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFS4445813.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33528..33791)
                     /gene="ilvM"
                     /locus_tag="ACHY5W_000872"
     CDS             complement(33528..33791)
                     /gene="ilvM"
                     /locus_tag="ACHY5W_000872"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFS4445814.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33788..35434)
                     /gene="ilvG"
                     /locus_tag="ACHY5W_000873"
     CDS             complement(33788..35434)
                     /gene="ilvG"
                     /locus_tag="ACHY5W_000873"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFS4445815.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPYAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     TQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSNGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35437..35487)
                     /gene="ilvX"
                     /locus_tag="ACHY5W_000874"
     CDS             complement(35437..35487)
                     /gene="ilvX"
                     /locus_tag="ACHY5W_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFS4445816.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(35574..35672)
                     /gene="ilvL"
                     /locus_tag="ACHY5W_000875"
     CDS             complement(35574..35672)
                     /gene="ilvL"
                     /locus_tag="ACHY5W_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFS4445817.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36024..37544
                     /gene="yifB"
                     /locus_tag="ACHY5W_000876"
     CDS             36024..37544
                     /gene="yifB"
                     /locus_tag="ACHY5W_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFS4445818.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37568..37906)
                     /gene="maoP"
                     /locus_tag="ACHY5W_000877"
     CDS             complement(37568..37906)
                     /gene="maoP"
                     /locus_tag="ACHY5W_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFS4445819.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38025..38864
                     /gene="hdfR"
                     /locus_tag="ACHY5W_000878"
     CDS             38025..38864
                     /gene="hdfR"
                     /locus_tag="ACHY5W_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFS4445820.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(38960..39035)
                     /locus_tag="ACHY5W_000879"
     tRNA            complement(38960..39035)
                     /locus_tag="ACHY5W_000879"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39000..39002),aa:Trp,seq:cca)
     gene            complement(39044..39120)
                     /locus_tag="ACHY5W_000880"
     tRNA            complement(39044..39120)
                     /locus_tag="ACHY5W_000880"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39084..39086),aa:Asp,seq:gtc)
BASE COUNT         9594 a        10666 c         9871 g         9035 t
ORIGIN      
        1 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gatgacatga
       61 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cgtcttgttg ttgctgtcgc
      121 cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcacgcaa
      181 aggctttgcc ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc
      241 cgacaacatg atgccagtaa tgcagcatcc ggcgcaggat aaagaaaccc agcagaagct
      301 ggcagaactt gagaaaaaaa ccggtaagaa accgaatgtg gttgttttct tgctggacga
      361 tgtgggctgg atggacgtcg gttttaacgg tggcggcgtg gcagtgggta accctacacc
      421 agatatcgac gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccaag
      481 ctcttcccca acccgcgcca ctattctcac cggacaatac tccatccacc acggcattct
      541 gatgccgcca atgtatgggc aaccgggcgg gctgcaaggg ttaaccacgc tgccgcagtt
      601 gctgcacgat cagggctacg tcactcaggc catcgggaaa tggcatatgg gggaaaacaa
      661 agagtcgcag ccgcagaacg ttggctttga tgatttccgt ggctttaact cggtgtctga
      721 tatgtacacc gaatggcgcg acgttcacgt aaacccggaa gtggccctga gtccggaccg
      781 ttctgaatac atcaagcaat taccatttag caaagatgac gttcatgcgg tgcgcggcgg
      841 cgaacaacag gccattgccg acattacgcc gaaatatatg gaagatctgg atcaacgctg
      901 gatggaatat ggcgttaagt tcctcgacaa gatggcgaag agtgataagc ctttcttcct
      961 ctattacggc actcgcggct gtcacttcga taactacccg aacgccaaat atgcgggtag
     1021 ctctccggca cgcacctcgt atggcgactg catggtggaa atgaacgatg tgttcgctaa
     1081 tctgtataaa gcactggaaa aaaacggtca gcttgataac acgctgattg tctttacctc
     1141 cgataacgga ccggaagccg aagtaccgcc gcacggacgc acaccgttcc gtggtgcgaa
     1201 aggttcgacc tgggaaggcg gcgttcgcgt tccgactttc gtgtactgga aagggatgat
     1261 ccaaccgcgt aaatctgacg gtattgtcga tctggcagat ctcttcccta ccgcgctgga
     1321 tctggcaggg catcctggcg cgaaagtggc gaatttagtg ccgaaaacca cctttatcga
     1381 tggtgtggac cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga
     1441 gcactacttc ctcaacggta aactcgctgc tgtgcgtatg gatgaattca agtatcacgt
     1501 cctgattcag caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac
     1561 agtaatgcaa acagccggat cgtcggtgtt taacctgtac accgatccgc aggagagcga
     1621 ctctatcggc gtgcgccata ttccgatggg tgtaccgcta cagaccgaaa tgcacgcgta
     1681 tatggagatc ctgaaaaaat atccaccacg cgcgcagatt aaatctgatt aagccggcga
     1741 tatcgctaca tcgggccaca ctgaccttgc cggatgcggc gtgaacgcct tatccggcct
     1801 acaagccttg caaattcaat aaattgtagc gttctgtagg ccggataaga tgcggtcagc
     1861 atcgcatccg gcaatcaacc gcaggcggcc gccgatttct acttactcac caccagcaaa
     1921 tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta
     1981 agatatggcg gtagatggcg gaaataacgc tgatacccgg cacacaaata attcagcccc
     2041 ggtttaccgc tggcattgag catgaagcgg tgtttcgggc agcctcccca gcacgctttt
     2101 aacacgttgc aactgcgaca ctgcgccggt aactgcttaa atttatcttc accaaaagcc
     2161 tgctgctgcg gagaatctac catttctgca attgtttgct ggtgcatatt ccccaaccga
     2221 tattgcggat aaacatagtg atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc
     2281 gagcgcccac aggttggctg atgatggcaa accgcacccg gcgcaccgac aaaattggca
     2341 aacgcccatt cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat ccagtgatcg
     2401 aatatcgcca ccagaaattc accaaattca tcggggcgca ccgaccattc cgtcagttcc
     2461 ccctgaatat caccaggcgc atgtagtttc agtccatcgc gggcagctgt ttcatcagcc
     2521 aggcgctcga ccaccggaat aaactggatg aattccactc ccgcatcgca caaaaaatca
     2581 taaacctgca acggttgctg cgcgctggtg cggttgacgc agaccagcac gttatagtcg
     2641 acatgatgtt tttgcagcag cgtcagggca cgcatcacca gcttatgcgt tggtctgcca
     2701 cctttggtca cgcgatattg attgtggatc tcagccggac catccagcga taacccaaca
     2761 agaaaatgat tttctgccag aaatgcacac cattcatcat cgagcagtac gccgttagtc
     2821 tggaagctgt tacttatctt cctgccagca ccatatttcg cctgtagagc cacggcacgg
     2881 cggtaaaaat ccagcccgag taacgttggt tcgccgccct gccaggtaaa agccacttcg
     2941 ttttgggttt cgctggcagc gatatagtga cggacatacg cttccagcgt gtcatcgtcc
     3001 atatgcgtga ctggcttttc gcggtaaagg gattgttttt cgagataaaa acagtagtca
     3061 cagttcagat tacaatcgga accgctcggt ttcgccatca catgaaaagc acgcgttgga
     3121 acctgttgca gcatggtcgc tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa
     3181 tcataccgtg gggcaatgag ttattacggg cgtttattgc gaagggaggg tgacgaaatg
     3241 cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga gagaggattc
     3301 gaacctccga cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa
     3361 tactgctttt tgaattttta gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg
     3421 aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac
     3481 cttcgcattg tcacaacttc taataatggg gtggctaatg ggattcgaac ccacgacaac
     3541 tggaatcaca atccagggct ctaccaactg agctatagcc accactacaa atcttgttac
     3601 gcggtattac taccaccgca gctcaagcgc cgggactaaa tggcgcgccc gacaggattc
     3661 gaacctgaga cctctgcctc cggagggcag cgctctatcc agctgagcta cgggcgctta
     3721 gcgccgttgc gggggtggat aatacggact tcccacccct ctgtctagtc cctttttaaa
     3781 taaattgcgc atttggttac ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc
     3841 tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc gtcaccgcca gcataaagat
     3901 gatgccaaca aacagcgaca tacgcgtatc ttcattaaag tacatgccga tcaaaacgca
     3961 aatcaggaat gccatcgtta cgtagttggc ccacgggaac aggattgagc ggaacggatg
     4021 gctggcaatc gccgctttat gtgcacgccg aaaacgcagc tggcttatca atatcacaaa
     4081 ccatggcacc atccccggaa gcacgctggc actgtagacg tagacaaaca cacgctgcgg
     4141 attgggaatg atgtagttca ggcatgagcc aattagcaga atagcaatag atactgccac
     4201 acccgcaact ggtacgccgt gacgggaaac tttcgccatc gccgccggta actgacggtt
     4261 tttcgccagt gcgtagagca tacgtccgca actgtacatg ccgctgttac agccagagag
     4321 cgcagccgtc agcaccacaa agttgataat gcccgctgct gcggtaatac cgattttggc
     4381 aaaagtcagt acgaacgggc tgccgttgct gcctatttca ttccacggga agatggtgac
     4441 gataacgaaa atcgcgccta cgtagaaaat caggatccgc cacagcacct tgcctacggc
     4501 actgcgcagc gtcacctgcg gattcttcgc ttcaccggca gtaatgccaa tcagctccac
     4561 gccctggtag gacgccacca caatacacag tgcggtcagg aaccctttcc agccacccgc
     4621 aaagaaaccg ccatgctctg tgagattgct aaaaccaatc gactgcccgc cattgccaaa
     4681 gccaaagaaa atcacgccca ggccaatgac aatcatcacg ataatcgtgg tgactttgat
     4741 catcgcgaac cagaactcga tttcgccgta caaccgcacc gccgccagat tcgccaacgc
     4801 caccagcgcc actgcgatca atgcgggtat ccactgcgcc atctccggga accagaactg
     4861 gacataaacg ccaatggcgg tgatttcaga gatccccacc gccatccaca taaaccagta
     4921 agaccaggcg gtgagatagc caaagaacgg gctcatataa cgatgcgcat aaactgcgaa
     4981 cgaaccggta accggttcga ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac
     5041 gaacagcccg gcgatgatat aggccaacaa tacggatggc ccggcccatt tcagggtact
     5101 ggcggccccc ataaacaggc cgacgccaat ggtgcccccc agggcgatga gttcgatatg
     5161 tcgagcttcc agcccacgct gtagctctgg tttgttatct gccataaatc ctcgtgttgt
     5221 gtttgcatgc tttccggtgt taccggttat cgttatgggt acatcgagtg ttgcgaatgt
     5281 tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata
     5341 aaaaagcaga caggcgacga agtgaccact ccgtcgcttt acaaagagag gaaaatcaga
     5401 ggttgccggt gtagtgccag cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc
     5461 tcggctgcga aagcaggcgg tagagccact ccagtcccag cgtttgccag attttcggtg
     5521 cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc aacgcccata tacagcgcat
     5581 ctggatgcac cagacggcag tcgcgcatga agatctcctg ctttggcgat cccatcgcaa
     5641 cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg
     5701 gtttaaaata accatcctga ctgccaacga tattcacatt ccattggttg cgcagtttag
     5761 cttcagtttg cgccagcact tcaggtttac cgcccacaag aaatactggc gtcccttctt
     5821 tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg
     5881 cctgcgggta ctttttacgt actgaacgta caacgctgat gccatccgca tatttaaatt
     5941 cggcagcgtt aattaactcc ctgacctcgg cgttatcttc aatggtcagc attttttcag
     6001 cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg
     6061 cgtgctgcat atcacgccaa ccaatcaact gtaagccacg cagcgtatag gtcggtgccg
     6121 tggtgttgtt attcattgtt atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt
     6181 atgaatgagt ccggcgcttt caaaaagcca gtacaacagt tttgcgatca tcagacatgc
     6241 gccgaagacc acgataaaaa agactacgcg tgagacaaac gaatccagcc cttcacgcgc
     6301 cagcacgatc atattgaaga ttgccccgaa gcagaaactg tgcaatatcg cagccttata
     6361 gcgattagtt tcgcggttgc ccagctcata cagccagtcg aaccatttga tgatcagacc
     6421 aaccacaatc gcccccagag gaataaacag cacgccgccc ataacaacca gtgaaccaat
     6481 aagcgtaggc gagatcgcca gaccggagtg gttattcagc acttcccagg taaagtagtt
     6541 ggcggagttc agcaccagac tcgggcgccc cggccacagc caggaaggga taaagacata
     6601 gaaatcgcgg acaattggcg ccagtccctg gaagtcgatg ttgtcgtagt tctgcaacag
     6661 caacgccaga ttctcccacg gcgagaaggt gtcgcgagtg agatagagaa atgtatagaa
     6721 cgcctcatcg ccgctcacgt tcattccgta gcgttttagc gccagccaga acatgccaac
     6781 aatccccagc acgcccgccg ccgccagcat ccacagtgaa atccagccgc gaataatgcc
     6841 gataaacagg aagatagcga atgcgatgat gatattggcg cgagtaccgc cgacaatcat
     6901 gtaagtcagc aggccaaagg cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc
     6961 ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa
     7021 cgccacgccg gagacttcac tggaaaagat ctgactgtag gagttaagcc ggaacagcaa
     7081 aaagccgttg tgcatgaaga agatgccgac gcttaccagc gcgataccca tcaggatcac
     7141 ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc
     7201 aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa accgcgtaga agcagcccgc
     7261 agaaagcaac gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa agcgaaatac
     7321 cagcacgctg gtcagcggga agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa
     7381 gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag
     7441 cgtgcagagc agccagacaa caaacaggcc actgaattgc agcagactca tgcaacctcc
     7501 ccggcggcaa tagccagcgc ccgctgccag ccttgcagat agttcgggct aaagaaggca
     7561 atggtgtttt tatccaccga cgccaactgg cgctgcgctt cacgcacaat atcctcgttg
     7621 agatcgtcgg tagtaaacag caccggcaaa tgctgttccg tcatatcctg ccagaacgga
     7681 ttttcccggt taagcacaca aggaatgccc gcctgaatca acaagcacag cgtgccgatg
     7741 ccctgctggc gggcaaaaat aaagtaaccg agatcgcact gacgaagtag cgtcagatag
     7801 gcgtcaaatt ccagtttttc gctcagaact tgcagatttt cttcgctgaa taactccagc
     7861 cccgcctgac gaacttcctc gatgtacgct tcgttattag gcggatatcc catcggcacc
     7921 accactttta ccgtatcgcc aaattgctga tgaacggcgc gcaaggcagc aatatgctca
     7981 ttgctgcggt cgccggagtt ccccaccaga atggtcattt tcccttcacg ttgccgatcg
     8041 ttcgccatcg tattgagaga aggatccatc cgcgtcggga agtacagcag ttcgccccgc
     8101 acctttggat gcgttttggc aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg
     8161 actcgctttt gcgccaggcg acgtagcggg taaaaaagct tatatctcaa gccactggaa
     8221 agctcataca gatctgcccc ccagatatgc cagaaaaact ggcgggactt aatgccacca
     8281 ctcagcaaag ccagccacag tgtgggattg aactgaccgt ggaagaaaaa acgctgctga
     8341 cggttagctt ttgctttcgc gatgaccgct tccgccagcg attttttccc agggaaaaat
     8401 tgcacagaaa gcgccggaca gctatcactt aagccgtcgt ccttgccaac aaccataaac
     8461 tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg
     8521 ttatggtgag ggatatccga tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc
     8581 agagtaaaaa cacgccacaa caaagagaaa aatagacgat ataggttgcc atataggcct
     8641 gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc atcaataaag
     8701 tgaactggct gacttccgcc agaatataaa accgcagtga agctttggcg atcaccagat
     8761 aaccaaagac ataagcgccc acttttaaca catcacccac caactgccag gcaaagagat
     8821 cgcgcatagc ggtaaattta ttcgacaaca gcagccagat agcaaaatca cgcagcaacc
     8881 agacggtaaa actcgccgcc gccactgccg gtaagacgaa tttcagcgat ttaaccactt
     8941 cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt gggcagtaaa tatacgctga
     9001 acgatgccgt aataaattgc aggtaggcat cggaaatact gctcacccct tgccagatcc
     9061 ccacttcatc ccagctatac tgcgccgcca gcagtttacg catcatgatg taagcaacag
     9121 gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt gctcaactgc cctgccagac
     9181 cgttatccca gctgggtttc agatagctta acgggatgac accacgtttg atcaacatga
     9241 tggcggcagg aattaccacc agcgcgggaa tcagcgccag acccagcaac gccccttcat
     9301 aaccgcccaa acggtaactg acgtagtacg caagaacgcc aatcaagctg ccaacaatca
     9361 gagataacgc attacctgcg gcatcgcgaa agcctttcat caacgccagt aacaggttgc
     9421 cccaggcaat ccccatttgc accaacgcca ctaaacgtac cagcccctga tagtcggtat
     9481 taccaaacaa tccctggctg attggcgctg ctgccagcac aaaaaccagc gccatcagcg
     9541 tagagaaacc aagtaccatc gctgatgaag tgccgaccac gcggcgcagc tgttgcggat
     9601 tatcatggta ctgggcaacg tatttggtta caccgttaaa gatgccagcc ccggcaagca
     9661 cgccgagcac ggtaatcaac tggcggaaat ttgccgccag cccaagcccc gcaggaccaa
     9721 atgacaccgc cagcaactta ccgaccagta acccggcccc aatcttgacc agtgtactgg
     9781 ccgccgtcca caaggacgct ttcgccaacg acatatcagg agaagtagtt caacaaagtc
     9841 gcaattaccg tacgctgatt gacgggcgac aggttgtaga acagcggcag gcgcagcagg
     9901 cgctcgctct ctttggtggt gtagcgatct tcaccgtgga actcaccaaa gcgttccccc
     9961 gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca taatttccgc ttctttcaga
    10021 aagttaatca acgcgctccg gtcatcaata tcccgcagtt taatgtagaa catatgcgcg
    10081 ttctgcacgc agccatcggg aatcgacggc agctcgatac gcccggcttt cgccagaggt
    10141 gctaacgcat cgtagtagtt ttgccacagc gccagacgtt gctggttgat acgatccgct
    10201 gcttccagtt gcgcccacag gtacgcagct tgcagatcgg acatcaaata gctggagcca
    10261 atatcgcgcc aggtatattt atcgacctga ccacggaaga actggctgcg gttagtgccc
    10321 ttttcacgga tgatctcggc tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg
    10381 ccttcaccac ccgccgtgta gtttttggtt tcatggaagc taaagcagcc aatatgacca
    10441 atggttccca gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc atcttctacc
    10501 acaaacaaat tatgcttttt tgccaacgcc ataatggtgt ccatttcgca ggccacaccc
    10561 gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt
    10621 tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc
    10681 acaaaggcat tggcggtgga gacaaaggtg tagctcggca tgatcacttc atcgccaggc
    10741 tggatatcga gcagcagcgc cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac
    10801 actttggcgc tgccaaaacg ttgctccagc cactgctggc agcgacgggt aaaaccgcca
    10861 tcgccacaca gtttgccgct acccattgcc gactgcatat agtcgagttc ggttcccacc
    10921 accggcggtg cgttaaatgg aatcatgtga tcacctgtat aaccagtacg cggtgctttc
    10981 tacattcgca ccactttgta tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc
    11041 cacccgcaaa gttgttttac cgcgagcata cgcccagttt agcgccgttt gcatcagctc
    11101 agcacctgca ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg
    11161 taaagagaca tagccgcgaa tatcgccgga cgccgcacgt aaaatcagac attgatgatc
    11221 aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa aagcgactgc tggcgtcagg
    11281 cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta actgacgtaa
    11341 tgcgggaata tcggtctctt gtgccactac agcaccgcta tcactggcat tgttcacggg
    11401 tagcgccaaa tcaacttcac cttctaccag ggagaatccc agctgttgca gggcatccag
    11461 ttcacccgta tttgatgccg caattttggc ctgcacccgt gaccacggcg ctaacgcgtc
    11521 tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa
    11581 ggcgttttcc caggttagtg gctcaatact ggcgcggacg ggcacgaagt aactccagca
    11641 gatattggcc gtagccagtt ttcgctaatg aactggcagc acgcttcaca ccctcgtcat
    11701 cgagccagcc gttacgccag gcgatctctt ccaggcaggc aatcttaaag ccctgtcgtt
    11761 tttccaccgt ctgtacaaag gtgctggctt caatcaggct gtcgtgagtg ccagtgtcca
    11821 gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg aggtacatct
    11881 ggttgatgga ggtaatctcc agttcaccac gctccgacgg cttcacctgc tttgcgtact
    11941 ccacgacttt actgtcgtag aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt
    12001 ttggcttttc ttccagcgag atagcgcgga aattgtcgtc aaactccacc acgccaaagc
    12061 gttccgggtc catcacctga tagccaaaaa ccgtcgcccc ttccgtgcgc gccgcaacat
    12121 gacgcagctt cggactgaag ccctgaccga agaagatgtt atcgcccagc accagacaag
    12181 aaggttcacc attaaggaag gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc
    12241 tgggctgttc ggcatattcc agctggatac cgaactcact accatcgccc agcagacgct
    12301 ggaaataacc tttatcttcc ggcgtagtga tgatgagaat ttcgcggata ccggccagca
    12361 tcagcaccga cagcggatag taaatcattg gtttatcgta aatcggcaac agttgcttcg
    12421 atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg ataatacctt
    12481 tcatgtttgc ctccgctgaa aattagcctt tcagacctaa acgctcgccc tgatagctgc
    12541 cgtcctgcac ctgcttccac cagctttcat tagccagata ccactgaacc gttttacgca
    12601 ttccactttc aaaggtttcc tgcggcagcc agccaagttc acgggcaatt ttcgaagcat
    12661 caatggcata acgcagatca tgccccggac ggtcagcgac aaaggtgatc aggtcacgat
    12721 agtgcgccac gccgtgcggc ttgttcggag ccagttcttc cagcagctcg caaatggttt
    12781 ccacaacatc gagattctta cgctcgttgt gaccaccaat attataggtt tcaccgactt
    12841 tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac agccagtcac
    12901 ggatttgctg cccgttgcca tataccggca gcgatttacc cgccagcgcg ttgaggatca
    12961 tcagcgggat cagtttttcc ggaaagtggt aaggaccgta gttattcgag cagttggtga
    13021 taagcgttgg cataccatag gttcgcagcc aggcgcgcac cagatggtcg ctgctggctt
    13081 ttgacgcgga ataagggctg ctcggcgcat acggcgtggt ttcagtgaag aaatcatccg
    13141 tcgagtgcag gtcaccatat acttcgtcag tggagatatg atgaaaacgg aacgctgatt
    13201 ttttatcttc cgtcagcgca ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc
    13261 cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat agaacggtca acatggcttt
    13321 cggctgccag atgcatgaca cagtctggct gatgctcagt gaatacgcgc gccagttctg
    13381 cccgatcgca gatatcaact ttctcaaagg aaaagcgctc gctttgcgcg accggtgcca
    13441 gcgacatcag gtttccggcg taggtcagct tatcgaccac taccaccgcg tcgctcgttt
    13501 cgttgatgat ataacgcacc agcgccgagc caataaaccc ggcaccacct gttatcagaa
    13561 tttttctcat cagcgccaga ctcctttggc atcgacgaca tactgctgat ggacattgtc
    13621 gccattgata actttgaact gactatgatc gaccagcatc accagcacgt ctgccgttgc
    13681 cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc agttttttcg gcaactggtg
    13741 gatgttaggc tcaacaacca gagtttcgcc gctatgccac tgggcgatca gttcagcgat
    13801 ttccatcgcc gggctttcgc gcaggtcatc aatattcggt ttaaacgcca gaccaaagca
    13861 ggcgattttc agttcactgg cgcgtttatc ggtagccgcc aggcaatcag ccaccgccgc
    13921 tttcacctga tcgataaccc agaacggttt gtgatcgttc acttcgcgcg cggtacggat
    13981 aagccgcgcc tgctgggggt tctgtgccac gataaaccac ggatcaacag caatgcagtg
    14041 accgcccacg ccagggccgg gctgaagaat attaacgcga gggtgacgat tcgccaggcg
    14101 aatcagttcc cagacgttaa tcccctgatc ggcacaaatc agcgacaatt cattagcaaa
    14161 agcgatattc acatcgcgga agctgttttc ggtgagctta cacatttccg ccgtccgcga
    14221 gttagtgacg acacactcac cttcgaggaa aattttgtac agttcgctgg cgcgggccga
    14281 acaaaccggc gtcataccac caatcacgcg atcgttttta atcagctcga ccataacctg
    14341 gcccggtaac acgcgttccg ggcagtaagc aatgttgacg tccgcctgct cgcccacctg
    14401 ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat tctgccatct tctcggttga
    14461 ccccaccggc gaggtggatt caaggatcac cagcgcgcct tttttcagca ctggcgcaat
    14521 ggagcgagca gccgattcaa cgtaggtcat atctggctca tgatcgcctt taaacggcgt
    14581 gggtacagca atcagccagg catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc
    14641 gccttctacg gcagttttta ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc
    14701 acgattgatg gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct gtttttgccg
    14761 tgaggcaaac gctgctgctg taggcagccc gatgtaaccc agtccgataa cagaaatggt
    14821 cgcaaaactc atagtgatat ccgattattt tttaacgctt ccagaatgcg agagcatgcc
    14881 tgaccatcac catacgggtt atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt
    14941 aaaagacgcg tcacttcctc gacaattcgc tgcttatccg tgcctaccag acgcaccgta
    15001 cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac cggtttcccc
    15061 agcgaaggcg cttcttcctg aatgccgcct gagtcggtca aaatcagcca ggcgtggttc
    15121 atcagccaga caaacggtaa atactcctgg ggatcgatca gaatgacatt tttcacatgc
    15181 cccagaatgc gattgaccgg ctctctgacg ttcgggttga gatgcaccgg atagacaatc
    15241 tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcgt ggcagatttc ttcaaagcca
    15301 cgaccgaaac tctcacgcct atgaccggtc accagaatta tctttttatc ggggtcgata
    15361 aacgggtaat ttgccgccag ttctgaacgc agcgtgtcgc tgctcatcac ctggtcacgc
    15421 acccataaca gtgcatcaat gactgtatta ccggtaatga agattcggct atccgcaacg
    15481 ttttcacgca gcaagttttg ccgggaagtt tcggttgggg agaaatgata catcgccaga
    15541 tgcccggtca atgtacggtt agcctcttcc ggccacggcg aatagagatc gcccgtgcgt
    15601 aaaccagcct caacgtgacc aacaggaata cgctgataaa acgccgccag gctggttgcc
    15661 agcgtcgtcg tcgtatcgcc gtgaaccagc acgacgtctg gtttgaactc ggcaagaata
    15721 gattttagcc cttccagaat ccgacaggtt atctctgtca ggccctgtcc tggctgcatt
    15781 atgttgagat cgtagtcagg tacaatggaa aagagtttca gcacctgatc gagcatctcc
    15841 cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag gatcttttgc caacgcatgc
    15901 accaacggag ccatcttgat ggcttccggg cgcgtaccaa atacagtcag tactttcaca
    15961 tcgattctct tcgaataagc ggcgagcacc tttgcgctca ccgcagcagt gttgctattt
    16021 cgagcaacgg cgggttaatg cgacaccagc cccgatcagc cccccgacaa tgccccacat
    16081 aatcatcagg aaggcacgac gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa
    16141 atagcgatag gtctgaaaac gcggatccag ggttggacca acattcaggg tgtttaacat
    16201 ggcccgattc tgatcatagt cgagatcaaa ggccggaccg acggcctgta aattttccag
    16261 tcgagcctgg agcattggac gcccaagcag gaacatttct gaatcaggta attcctcggc
    16321 aggcacatct gtcgcactgc gcgaaatatt atgctgctca gcaattttca gcgcctgctc
    16381 aatgctgttc atccggcggt cgtagatggc tttcgccacc tcttcctgac gcttcacctg
    16441 agctttcatc tggatggtgc gtgccgccca tgcgcctttc agctcatcat tcagatggct
    16501 ggctgcacgc tggctggcaa aagcaacata ctgacgtaac aggttattag cgtcaggcgc
    16561 ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa
    16621 ctggatgttg ttaatcattt catccagcaa cgccgcatcg gctttgctgt tgcccaccat
    16681 ccgctgcttg taatagtcgg tttgcagcca gaactctctg cgggtatccc acgaggccag
    16741 ttgcataaca aactctttat aggcttcgtc catgaccgat ggttggtcgg cagaagccat
    16801 gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat
    16861 attcaccgtt ggacgatcgg taatcgccgt cgagctccac tcctgacgag caaaaaacgt
    16921 atacgccagc gcgattaacg caaacgccag ccccatgcca ataatccata gcttcccagc
    16981 ccacaaggta cgaaacaacc cacgaatatc cagttcattt tcagcgtctt cggccggttt
    17041 cccaggcatt ggttgtgtca tcacatcctc atttatttgg ttaaattggg gctgccacca
    17101 cgatttctac gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc
    17161 ttaatgcaat atccatagag gaagaatgct agcaaaaaga gcaccagcat gacccactcc
    17221 gggacaaaat gagaatattc tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg
    17281 gtaatcagca caaacgcctg gcgggaagta aacccggcac gcatgatcaa atggtgaata
    17341 tgctgacggt caggagagaa tgggctcatg cctttacgca ggcgacggta cataatcgcc
    17401 accatatcca ttagcggaat ggcgattatc cacaaagcgg taaccgggct gatgggatgg
    17461 gttttgccct gggtcgtttc gagcaggatc cagatcacgg taaaaccaat cagcgtactg
    17521 cccgcatcac ccataaagac tttgtagcgg cgacccagga taccaaggtt aagcatgatg
    17581 tatggctgga tggcggcgat catcgcaaag caccagattg cgaggctggt ttgcccgtcg
    17641 aaccacaaaa tcataccgat tgctgcaaac gagacgcagg acaacccgcc cagcaagcca
    17701 tcaatgccat caaccatgtt gaacgcatta atggccgccc agacggcaaa tagcgtcagg
    17761 aagtaaccaa acggtccgag caccatctcc caggagccaa agatataacc caggctactg
    17821 agataaagtt tgccgaacac catcataaca atgccaacag cggcctgtat ggtggcacgg
    17881 atttttacgc tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag cacaccggca
    17941 caagcgagat agagagatgc atgcggaata tagtaatcga caattccgaa cgtgaagcaa
    18001 atccctgcgt aaaccgaaat ccccccaacg agaggtatca atccctggtg acgtttgcgg
    18061 aagtttggtt tatccactaa accgactttt tttgccacct tacgggcaaa aaacagaaac
    18121 agtgtcgtga ataaaaaaat actgatgaga tcagtactca ctgtcagtaa attcacaatg
    18181 catgctctca gagaaaatta ttagcagaag tataacctcg aagaccttta ttcagaaggg
    18241 aaagtccgtt ccgaacccac cagccctgtg caaaaattaa ccatttgata tgcaaattgc
    18301 ataacatgcc agcaaattcc aggaattttc ctaacatcgc cagcgcggca ttaagattac
    18361 agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt tggcataaga caaatttatg
    18421 agcgtttcat catttcgaag aaatcgtcat tggtcttggt cattgccagt ttattaatga
    18481 ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg atccacattt
    18541 tctgcagttc ttcctgagtc gtgagcagct cttctttacg ggtaccggaa cggttgtagt
    18601 cgatagccgg gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt tccatgttgc
    18661 ctgtaccttt aaactcttcg taaataactt cgtccatttt agaaccggta tcgataagcg
    18721 ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt acgcgccgca ccgaagaagc
    18781 gtttcggacg atgcagggcg ttggcatcca caccaccggt caacacttta cctgacgccg
    18841 gaacaacggt gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga atgataacgt
    18901 ctttcttgtg ctcaaccagg cgtttcgcct tctcgatcac catttccgca acctgaacgt
    18961 ggcgagatgc gggttcgtca aaggtagaag caacaacttc accttttacc agacgctgca
    19021 tctcggttac ttcttccgga cgttcgtcga tcagcagaac catcagcaca caatccgggt
    19081 ggttgtaagc aatgctctga gcaatgttct gcagcagcat ggttttaccg gctttcggcg
    19141 gtgccacaat cagaccacgc tgaccacgac cgataggtga tgccagatcc agtacgcgag
    19201 cagttaaatc ttcagtagaa ccgttaccac gttccatacg cagacgagag tttgcgtgca
    19261 gcggggttaa gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa
    19321 cttcgttaac tttcagcagc gcaaaatagc gttcaccttc tttcggcggg cgaatcttac
    19381 cagagatggt atcaccagtg cggaggttga aacggcggat ttggctaggg gaaacgtaga
    19441 tgtcatcagg accggcgagg taggagctgt ctgcggaacg gaggaaacca aatccatcct
    19501 gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc actctttgcg tgctgcttca
    19561 ggatggcaaa aataatgtcc tgcttacgca tacgagccag gttttccagc cccatatttt
    19621 cgccgagagt gatcagctca gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg
    19681 tgtgagttct taaacttggg gtaaatctcg aacttaatgt tgtgaatggt atggcaggat
    19741 catccatgcc tgtttacggt catcgactca tgtcttttcg ctgcctggtc acaggagagt
    19801 acgcagaact gaaacgacaa gacggattga gtgacgagcc agaaatctgt tcacttcgca
    19861 tttaagataa aatgggaagc gttggaaatt acaagattca aacttaataa ggtatgttta
    19921 atacgaagtc aacactaagt tagcatgact cacgccgggc gtccagtttt tagcgacggg
    19981 gcacccgaac atgaaattcc cttacgccag gttagcgtcg aggaactctt tcaactgacc
    20041 tttagacagt gcgcccactt tggttgccgc cacttcaccg tttttgaaca gcagcagagt
    20101 cgggatacca cggatgccat atttcggcgc agtgccaggg ttttgatcga tgttcagttt
    20161 tgcaacggtc agtttgccct gatattcgtc agcgatttca tccagaatcg gggcgatcat
    20221 tttgcacgga ccgcaccact ctgcccagaa atcgacgagg atcgccccgt ccgctttgag
    20281 tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca tatataactc
    20341 cacaggaata agcctggcgt gttggtttcg ttgttggtgt aacattaacc aactaaaggt
    20401 tgactttatt tcaccggata cgctttcgta aagcaatagt aagctgatat tctaccacac
    20461 tatgagcaaa acacatttaa cagaacagaa gttttccgac ttcgccctgc atccgaaggt
    20521 tgtagaagcc cttgaaaaaa aagggtttca taactgtacg cccattcagg cactggccct
    20581 tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac
    20641 gatggcgttt cttacgtcaa cgtttcatta tcttctctct catcctgcga ttgccgatcg
    20701 caaggtgaat cagccgcgtg ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat
    20761 ccatgccgat gcggaaccgc tggcggaagc taccggcctg aagctgggtc tggcttacgg
    20821 tggtgatggt tacgacaaac agctgaaagt gctggaaagc ggcgttgaca ttctgattgg
    20881 caccacgggt cgtttaattg actacgccaa gcagaaccac attaacctcg gtgccattca
    20941 ggtagtggta ctggacgaag ccgatcgcat gtacgatctg ggctttatta aagatatccg
    21001 ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac
    21061 gctttcgtac cgggtacgtg aactggcgtt cgagcagatg aacaatgccg aatatattga
    21121 agtggaaccg gaacagaaaa cgggccaccg tattaaagaa gagcttttct acccttctaa
    21181 cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag acagagcgat
    21241 tattttcgcc aataccaaac accgttgtga agagatctgg ggccacctgg cggcagatgg
    21301 tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga
    21361 tgaatttacc cgtggcgatc tggatattct ggttgccacc gacgttgccg cgcgtggttt
    21421 gcatattccg gcagtgacgc acgtctttaa ctacgattta cccgatgact gtgaagatta
    21481 cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc
    21541 gtgtgaagag tatgcattga atttgcctgc tattgaaacc tatattggtc actcaattcc
    21601 ggtaagcaaa tacaatccgg acacattgat gaccgatctg ccaaaaccgc tgcgcctcac
    21661 gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc
    21721 aggttaaata aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg caagagaatg
    21781 aatcctgaat cacttactgt agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga
    21841 cagattgaag gatgaagagt atgggttcca cctcgtcgct gtatgcagcc attgatctcg
    21901 gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc cagacgctga
    21961 cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat gccctctcca
    22021 atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata
    22081 ttcctccctc gcaaattcgc gttgtcgcta cggcgacgtt acgccttgcc gtcaatgcgg
    22141 gtgattttat tgccaaagcg caggaaattc tcggttgtcc ggtacaggtg atcagcggtg
    22201 aagaggaagc acgtctgatt tatcagggcg ttgctcacac cactggtggt gccgatcagc
    22261 gcctggtggt ggatataggc ggagccagta ctgaactggt aaccggcacg ggtgcacaaa
    22321 ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc tattttgccg
    22381 atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc gaagtgttac
    22441 gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt gcttccggca
    22501 ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc attaccctgg
    22561 aaaagttgca gcaactgaaa caacgcgcca ttcattgcgg tcggctggaa gagctagaga
    22621 ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg atcctgatcg
    22681 ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag
    22741 gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc cgtacgctgc
    22801 gtaatattca acgccgcttt atgatcgata ttgatcaggc acagcgcgta gccaaagttg
    22861 cggctaactt cttcgatcag gtggaaaacg aatggcatct tgaagcaata agccgcgatt
    22921 tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttc aaacaagcgc
    22981 cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc cccgcacaga
    23041 aaaaattgct ggcgacacta ctgctcaacc agactaatcc gatcgatctc tcatcgctgc
    23101 atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta ctacgcctgg
    23161 ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca ttacaggcta
    23221 accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat ccgctgggta
    23281 aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg ctggaagtgc
    23341 attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg attatttccc
    23401 tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt tctgcatccg
    23461 ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct gcggcagctc
    23521 cagcaaaaag cggctcggct ccgggcgcac cagttcgccg tactgacggc gttctttaca
    23581 cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca gccgccgctc
    23641 ctcatcgata ttatcttcat cgatactgct ctggtgcggc aaaaaccctt cttccatacc
    23701 gaccatgtag acataaggaa actccagtcc ttttgacgcg tggagagtca tcagttgcac
    23761 ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt
    23821 caccacctgg gtgagcgtca tcggctcatc cagttcactg ccttccagca tctccgtcat
    23881 ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg ggctgggcga
    23941 tgtttcgtac agccaggatt cataatccat gccatggatc agatcacgca ccgcggcaat
    24001 cggctcccgc tccgccagac gctggatttc tgccaaccag tgagtgaagc gggtcaatgc
    24061 ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg cggtaaacat
    24121 gcttttattg cgcgtcatcg cccattcacc caactttttc agcgtcgccg ggccaatctc
    24181 tcgcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac
    24241 gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg taccaccgga
    24301 tattttgtac gggatgcggt tttgcatcag gaatttttca aatacccgcg actgatggtt
    24361 gccgcgataa agaatggcgt aatctttgta ctgcgtttta ttgacgaagt gatgggcgat
    24421 cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta ttcgcgctta aaacttttag
    24481 ctctgtgccg taacccagtt cggagaacag acgcttttca aagacgtgcg ggttattggc
    24541 gatcaggatg ttcgccgctt tcagaatacg cccggaagag cgatagttct gctcaagctt
    24601 aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgcg gacgtgcacc
    24661 gcgccaggag tagatcgact ggtcatcgtc acccaccacg gtaaagcgcg cgcggctgcc
    24721 caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact catccaccag
    24781 caggtagcga attttgttct gccagcgttc gcgtacttct tcattgcgtt gcagcagcaa
    24841 cgtcggcaat aaaatcaggt catcgaagtc gagaacgtta caggctttca ggtgtgcatc
    24901 atacagccca taacaatggg caaaaatacg atcccgctcg ccctttgcct ctgccgccgc
    24961 ctgcgccgga gttttgagat cgttcttcca gttagagatg gtcgaaatca gttgttgcag
    25021 gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag caagctgatc
    25081 ggtatcgtca aacaacgaga agttcgcttt catcccaagc gccgcatact cgcgtttgat
    25141 gatatccagc cccaacgtat ggaaagtgga gatcatcagc ccacgcgcct ctttgcgccc
    25201 cagcgtctgc cctacacgct ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc
    25261 cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt tattggtgat
    25321 cacgcgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg taacgaattc
    25381 gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc ttcgaacggg
    25441 gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct aaccggttgg
    25501 ctgcggatgt ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg aatccattag
    25561 acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt actaaggaga
    25621 ggtttactcg tggcgattaa tttcagccct aaggttggtg aaatactgga atgcaacttt
    25681 ggtaactatc ctgttagcca aaatggaccg ttcagcacca cgtattacga tggccgcatt
    25741 cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca acggaaaaat taatggcaac
    25801 gcatgtatcg tcgttccatt atcaaccact cgcgaccatg acaagttaaa tcgtggaatg
    25861 catgttgaaa tagccagtaa tattatcaat gacctgcaat tctttgatca gcagattcgt
    25921 tgggccaaag ccgacctggt acaacaagtc agtcgaaaca gattaaacag agcaagaact
    25981 tacagagggt atcttaatca atgtttgcca catgagttgg ttgcagacat acagagggcg
    26041 gtcatcaagt caatcaatgc catatcgctc ataaattgac aataaaatca ccatccccga
    26101 taatgacttt gtagcccata gaggccctaa gttttctccc cttgagggac ctgcgaagcc
    26161 cggattttcc gggcttttgc catatatgta ccagcccctc cctgactgac aattacgtta
    26221 gcatctgcgg atcatgctaa aatcgccgcc tgacaattat tcactcaagg cacgatcatg
    26281 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    26341 ctcgagcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc
    26401 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    26461 ttcgataaag ttgtgttctc ttgtccggta ctggagccga ctggcccgct gcatacccag
    26521 ttcggttacc acatcattaa ggtgctttac cgtaactaac agtaaggcct tctccgggag
    26581 aaggccttga actctgaatc gctattaacc cgcaacagca atgcgtttca tatctgtcat
    26641 atagccgcgc agtttcttac ctacctgctc aattgcatgg ctgcgaatcg cttcgttcac
    26701 atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca ggtcgcccgg
    26761 ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga acagatagtt
    26821 accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct tacgggcgat
    26881 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    26941 gccggaatca accatggttt caaatgccag ctcaacgccc gctttcacca tcgcgatcat
    27001 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    27061 ttcaaacgcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    27121 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    27181 gaacagcggc gccatgatct ctttcagctg ttctgacagc gcgtaagcac gcagtttcgc
    27241 cgggttagag agacggtcca tcatcagggt gatgccgccc tgtttcagcg cttcggtgat
    27301 ggtttcccag ccgaactgaa tcagtttttc tgcatatgcc gggtcggtgc cttcttccac
    27361 cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagaa tggtttgctc
    27421 gcccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
    27481 accaccggtt gctgccgccc atgctttagc gatcgccata ccttcgcctt tcggatcgtt
    27541 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgcac
    27601 ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atatctttac ggatctgctc
    27661 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
    27721 tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    27781 atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt cggttgcttt
    27841 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    27901 accagaatca cgcatgttca ggccctggtt cagaccctgt gcgccacagc cgacgatgac
    27961 cactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg
    28021 acatttgccc agctgtgcca gctgctggcg cagattcagt gtattgaagt agttagccat
    28081 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
    28141 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
    28201 ctgcaacgtc aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
    28261 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca
    28321 cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta tttgtgcgcg
    28381 ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc gcccagcaaa
    28441 cgctgttgca gtatcagcag ctgcgccaca ccatcgatca gcaagggccg tcgctctctg
    28501 gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg ccgattctgg
    28561 atcgcttccg cgcggaacac ccgtcggtgg agattaaact tactactggt gatgcggcag
    28621 atgcgatgga aaaggtggtc actggtgaag cggatctggc gattgcgggt aaaccggaaa
    28681 ccttgcccgg cgcagtggcg ttttcgatgc tggagaatct ggcagtagtg ctgattgcgc
    28741 ccgcgctgcc ctgcccggtg cgtaatcagg tgtcggtaga gaagccggac tggtcaacgg
    28801 tgccgtttat catggccgat caggggccgg tacgccgccg cattgaactg tggtttcggc
    28861 gtaacaaaat cagtaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    28921 cgatggtggc gctcggctgt ggcgtggcgc tgttgccgga agtagtgctg gaaaacagcc
    28981 ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcaa cgatgaaaaa acgccgtttg
    29041 aactgggcgt ttgtgcgcag aaaaagcggc tgcatgagcc gctgattgag gcgttctgga
    29101 agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccggatta ttggtttcgt
    29161 cgtggcaatc gtagcccagt tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    29221 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaataaag
    29281 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    29341 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    29401 agcgcacatg caacttcgcc atttcgtcgt cggagagatc aaccacgctg tagccgccgt
    29461 cgttgagcat ctttaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    29521 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgagcgcc
    29581 cgccaagcag ctggcagaat ttgaggaagc tgcctttttc ttccgggatg gtgaccgcca
    29641 gcaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgaa
    29701 agttcacgtt agcaccggaa agaatatgcg ccaggcgttc gccgcgaatg ttgtgctgag
    29761 cgatatattt tttcattccc gccagcgcca acgcgccaga gggttccgcc actgcgcgca
    29821 catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg gtgatgatgt
    29881 cgtcgagata ctcctggcat aaacggaagg tttcgtcgcc gatgcgtttt accgcgacac
    29941 cttcggcgaa cagtcccacg cgtggcagat caaccggatg acccgcatcc agcgctgctt
    30001 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgcggc atcagttgtt
    30061 tgatcagcac cgacacgcca gcagccagac cgccgccgcc gactggcaca aatacgcggt
    30121 cgagatgagc atcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    30181 tcggatggtc gaacggcggc acccaggtga agccctgctg ctgtgacagt tcgatcgctt
    30241 tggctttcgc ttcatcaaag ttcgcgccgt ggagcagcac ttcgccgccg aagccgcgca
    30301 ccgcgtcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    30361 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    30421 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    30481 agctgtgcac tggctggcga tcttcgcgtt tcaccagaat cacgttatca agacgagacg
    30541 agagcttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtagacc ggtgcgcgta
    30601 gcaccgctct taaatattcg gcaccttccg gggcaccgga caggggttgt gagtcagcca
    30661 ttattaaccc cccagtttcg atttatcgcg caccgcgcct ttgtcggcgc tggttgccag
    30721 gctggcataa gcacgcaggg caaaggagac ctgacgttca cgatttttcg gcgtccaggc
    30781 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    30841 taactgaatg ccacggttcg gaatgtcgat ggcgatcagg tcgccgtctt caatcaggcc
    30901 aatgctgccg ccgcttgccg cttccggtga aacgtgaccg atggaaagac cagaggtacc
    30961 gccggagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    31021 gaaactggtt gggtagagca tttcctgcat ccccggaccg cctttcgggc cttcgtagcg
    31081 gataacaacc acatcgcccg cgacaacttt accgccgaga atcgcttcta ccgcatcgtc
    31141 ctggctttcg tatactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    31201 ggttttaacg atgcagccgt tttccgcgaa atttccgtag agcaccgcca ggccaccgtc
    31261 tttgctgtag gcgtgttcca gcgagcgaat acagccattt gcgcgatcgt cgtccagcgt
    31321 atcccagcgg caatcctgcg agaatgcctg tgtggtacga atgcctgcag ggcctgcgcg
    31381 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagcgtttg
    31441 cggcaacgtc aggccaagta cgtttttcac atcgcgattc agtaagttag cgcgatccag
    31501 ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttttg
    31561 ggtgctcggc gcaactttgc ataactgcgg gactttacgc gaaagtttgt cgatatcact
    31621 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagaa cggtgttagt
    31681 cgatccaccc atcgcgatat ccagcgtcat ggcattttca aacgccgctt tgctagcaat
    31741 attgcggggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    31801 gcgtttacca gcattgagga acagctgctt acggtcagag tgggttgcca gcagcgagcc
    31861 gttgcccggc tgcgacagac ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    31921 catcccggag caggaaccgc aggttgggca tgcggaacgt tcaacctgat cgctctggga
    31981 gtcagaaact ttcgggtctg ccccctggat catcgcatca accagatcga gcttgatgat
    32041 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    32101 aatattcaga cgcagagagg ccatcagcat ccccggggtg attttgtcac agttagagat
    32161 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    32221 ttcgcgagat ggcagtgaat aaagcatccc cccgtgtccc atggcaatcc catcatccac
    32281 cgcaatggtg ttgaactctt tggcaacacc accagccgct tcaatttgtt cggcgaccag
    32341 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    32401 cgcgataatc ggcttaccga aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    32461 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    32521 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    32581 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccggtg aagaggccga
    32641 agaaggcttg ctggatgcgt ttggtgaccg gaccacaacg gccttcgcca acctgaatac
    32701 cgtctacgct gcgcactggc gtgatttctg ccgctgtccc agacataaac acttcatccg
    32761 ccaggtacag tgattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    32821 ccagtttgat gatggcatca cgggtaatac ccggcagcgc tgaggaggtg aacggtgggg
    32881 tgaacagcac gccgtctttc acttcaaaca ggttttcgcc tgcaccttca gagatgtagc
    32941 cattcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttca ctacccacca
    33001 gcagggaaga gagatagtta ccaccggctt tcgccgcggt tgggatggtg tttggtgctg
    33061 cacggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    33121 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttaa
    33181 cgcccatacc aacatcaccg acgaagatca gcggacggat ataggcgctg gtgagattgt
    33241 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc gaaaccggga
    33301 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    33361 ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca
    33421 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    33481 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    33541 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    33601 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    33661 ttttgtgcat cgctggcggc ggccatattc attgagcaga cgtggaaacc acgatgacgc
    33721 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    33781 tgttgcatca tgataatttc tccaacattt ctgaattact ggcaccaggc ggcaccagcg
    33841 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca ttactgttca
    33901 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    33961 cgaaggcgct ggctaacttg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    34021 gttcctggaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    34081 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    34141 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    34201 caaccgccgc cggtaaacca aaacccatgg tacctaagcc gctggaggtg atgaaatttt
    34261 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    34321 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggcgcgtaga
    34381 tagcgtcacc gggatggtcg taacgccagg catgttcatc acgcagctgc gtgcagtgta
    34441 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    34501 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    34561 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    34621 gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc gctttggtgc
    34681 catgcattcc cagcatgccc agatagtacg gataatctgc ttcaactgcg cccagccctt
    34741 tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa gcaggaactg
    34801 cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct tttgccagca
    34861 tctggcgcgc ttgctcaact tcggcatatg ggaaagtcac ttcgttttca acggtggtga
    34921 accacggttc caggtcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    34981 gaccaggacg acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    35041 ccagcgactg caccaggaag ctgtgcttag tacaggctaa cgacaatccc aggacatcca
    35101 cttcctgaaa tgcgtcagtg ccgataaacg gtgcggacac ttgaccggtg atggcaacaa
    35161 cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    35221 cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg atagccgcca
    35281 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    35341 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    35401 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    35461 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    35521 aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc
    35581 tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg
    35641 accagactaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag
    35701 gaatgcctaa agagttacca tagattttgc gaatagcaca agatattttt ggccgatttt
    35761 tatgatggct ttaataattt caatagaaat tgttgttttt taaaaagaaa atagtgagtg
    35821 aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc ctttgtccgc
    35881 tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa
    35941 caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat cttatgcaca
    36001 taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc gcagccctgg
    36061 gagtaaatgc gcccccaatc actgttgagg tacatatcag taaaggtcta cccggcttaa
    36121 cgatggtggg cttaccagaa acaacggtaa aagaagcccg cgatcgcgtg cgcagcgcca
    36181 ttatcaatag cggatatgaa tatccggcca aaaaaatcac catcaacctg gctccagccg
    36241 atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct
    36301 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctggcgctta
    36361 caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct attaagtcgg
    36421 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    36481 gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt aagcacactc
    36541 tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg
    36601 ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc gggcacaacc
    36661 ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggtc
    36721 ttttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa
    36781 atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcaccg catcacagtg
    36841 cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgctgg
    36901 cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg
    36961 ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa
    37021 cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc ggacattatc
    37081 agggaaacca taaccgctgc acgccagaac agacattacg ttatctcaac cggctctcgg
    37141 ggccctttct cgaccgcttc gatctctcac tggagatccc attaccaccc cccggcattt
    37201 tgagtaaaac ggtagtgccg ggagaaagca gcaccaccgt taaacaacgc gtaatggccg
    37261 ccagagagcg ccaatttaag cggcagaata aactgaatgc ctggctggat agtccggaaa
    37321 tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gttggaagga acactgatcc
    37381 atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga accattgctg
    37441 atattgatca gtctgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgtg
    37501 cgattgaccg tttgctcatc catctgcaaa aactactgac ataaaaaagg gcatttcgcc
    37561 ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc
    37621 cggaaagggt gtgaaaacgt tttggacgct tgatacgcgt catatacttg gaccacacgc
    37681 gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt
    37741 cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct
    37801 caagcagttg tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat
    37861 gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta
    37921 attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac
    37981 aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt
    38041 taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct
    38101 atctgaccca atcagccgtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga
    38161 atcttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc
    38221 cttatgcaga aacgctcatg agcacctggc aggccgcccg taaggaggtg gcgcatacct
    38281 cacgacataa cgagttttct atcggtgcca gcgcctcatt gtgggaatgt atgcttaatc
    38341 agtggctggg acgtttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc
    38401 gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta
    38461 ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg tatttcactt
    38521 tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactgg
    38581 agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac gaagtgccca
    38641 ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca
    38701 cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca
    38761 caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatacgttga
    38821 ttcgcgatct gttgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa
    38881 ggatgccggt agaaggattt acttcagaga gggttatttc agataaaaaa aatccttagc
    38941 tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt
    39001 ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga
    39061 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
    39121 accgaattct tttacaacca ccggttttat gaccggctta ctgctt
//
LOCUS       DAZUGK010000019        38953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZUGK010000019 DAZUGK010000000
VERSION     DAZUGK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.019"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHY5W_000881"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHY5W_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4445821.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1477)
                     /locus_tag="ACHY5W_000882"
     CDS             complement(1358..1477)
                     /locus_tag="ACHY5W_000882"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFS4445822.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACHY5W_000883"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACHY5W_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFS4445823.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACHY5W_000884"
     CDS             2134..2217
                     /locus_tag="ACHY5W_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445824.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHY5W_000885"
     CDS             2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHY5W_000885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFS4445825.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHY5W_000886"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHY5W_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFS4445826.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHY5W_000887"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHY5W_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFS4445827.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGTV"
     gene            4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHY5W_000888"
     CDS             4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHY5W_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFS4445828.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHY5W_000889"
     CDS             complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHY5W_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFS4445829.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHY5W_000890"
     CDS             complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHY5W_000890"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFS4445830.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQATQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHY5W_000891"
     CDS             8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHY5W_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFS4445831.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHY5W_000892"
     CDS             9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHY5W_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4445832.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11333..12055
                     /gene="folM"
                     /locus_tag="ACHY5W_000893"
     CDS             11333..12055
                     /gene="folM"
                     /locus_tag="ACHY5W_000893"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFS4445833.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHY5W_000894"
     CDS             complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHY5W_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFS4445834.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12516..13235
                     /gene="rstA"
                     /locus_tag="ACHY5W_000895"
     CDS             12516..13235
                     /gene="rstA"
                     /locus_tag="ACHY5W_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFS4445835.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13239..14540
                     /gene="rstB"
                     /locus_tag="ACHY5W_000896"
     CDS             13239..14540
                     /gene="rstB"
                     /locus_tag="ACHY5W_000896"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFS4445836.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14616..15545
                     /gene="tus"
                     /locus_tag="ACHY5W_000897"
     CDS             14616..15545
                     /gene="tus"
                     /locus_tag="ACHY5W_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFS4445837.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHY5W_000898"
     CDS             complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHY5W_000898"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFS4445838.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHY5W_000899"
     CDS             complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHY5W_000899"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS4445839.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18933..20108
                     /gene="manA"
                     /locus_tag="ACHY5W_000900"
     CDS             18933..20108
                     /gene="manA"
                     /locus_tag="ACHY5W_000900"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFS4445840.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHY5W_000901"
     CDS             20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHY5W_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS4445841.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     FAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPISWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21767..23041)
                     /gene="uidC"
                     /locus_tag="ACHY5W_000902"
     CDS             complement(21767..23041)
                     /gene="uidC"
                     /locus_tag="ACHY5W_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFS4445842.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYSVFSMTLC"
     gene            complement(23080..24453)
                     /gene="uidB"
                     /locus_tag="ACHY5W_000903"
     CDS             complement(23080..24453)
                     /gene="uidB"
                     /locus_tag="ACHY5W_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFS4445843.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLQAFVIIWFYPLTDKKFNEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24450..26261)
                     /gene="uidA"
                     /locus_tag="ACHY5W_000904"
     CDS             complement(24450..26261)
                     /gene="uidA"
                     /locus_tag="ACHY5W_000904"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4445844.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQACNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNPSLGIGFEAGNKPKELYSEEAVNGKTQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(26652..27242)
                     /gene="uidR"
                     /locus_tag="ACHY5W_000905"
     CDS             complement(26652..27242)
                     /gene="uidR"
                     /locus_tag="ACHY5W_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFS4445845.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27471..28238)
                     /gene="hdhA"
                     /locus_tag="ACHY5W_000906"
     CDS             complement(27471..28238)
                     /gene="hdhA"
                     /locus_tag="ACHY5W_000906"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFS4445846.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28350..29378)
                     /gene="malI"
                     /locus_tag="ACHY5W_000907"
     CDS             complement(28350..29378)
                     /gene="malI"
                     /locus_tag="ACHY5W_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFS4445847.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29553..31145
                     /gene="malX"
                     /locus_tag="ACHY5W_000908"
     CDS             29553..31145
                     /gene="malX"
                     /locus_tag="ACHY5W_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFS4445848.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            31155..32327
                     /gene="malY"
                     /locus_tag="ACHY5W_000909"
     CDS             31155..32327
                     /gene="malY"
                     /locus_tag="ACHY5W_000909"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFS4445849.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMSKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVGRGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32431..33432
                     /gene="add"
                     /locus_tag="ACHY5W_000910"
     CDS             32431..33432
                     /gene="add"
                     /locus_tag="ACHY5W_000910"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4445850.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRVLMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(33468..34508)
                     /gene="ydgJ"
                     /locus_tag="ACHY5W_000911"
     CDS             complement(33468..34508)
                     /gene="ydgJ"
                     /locus_tag="ACHY5W_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4445851.1"
                     /translation="MSDNIRVGLIGCGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34751..34876
                     /gene="blr"
                     /locus_tag="ACHY5W_000912"
     CDS             34751..34876
                     /gene="blr"
                     /locus_tag="ACHY5W_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFS4445852.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            35149..35364
                     /gene="ydgT"
                     /locus_tag="ACHY5W_000913"
     CDS             35149..35364
                     /gene="ydgT"
                     /locus_tag="ACHY5W_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFS4445853.1"
                     /translation="MTVQDYLLKFRKINSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            35450..35890
                     /gene="ydgK"
                     /locus_tag="ACHY5W_000914"
     CDS             35450..35890
                     /gene="ydgK"
                     /locus_tag="ACHY5W_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFS4445854.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            35967..36548
                     /gene="rsxA"
                     /locus_tag="ACHY5W_000915"
     CDS             35967..36548
                     /gene="rsxA"
                     /locus_tag="ACHY5W_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFS4445855.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            36548..37126
                     /gene="rsxB"
                     /locus_tag="ACHY5W_000916"
     CDS             36548..37126
                     /gene="rsxB"
                     /locus_tag="ACHY5W_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFS4445856.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSELCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            37119..>38953
                     /gene="rsxC"
                     /locus_tag="ACHY5W_000917"
     CDS             37119..>38953
                     /gene="rsxC"
                     /locus_tag="ACHY5W_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS4445857.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKTRKLEQQQANAEP"
BASE COUNT         9485 a        10090 c         9516 g         9862 t
ORIGIN      
        1 ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccatccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggct atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc ggaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaag tcgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgtggtcca gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctct ctatggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggatcgg cccccgtgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagtcc
     1321 ggactgcggt aaatacccgt ccggacttat tgcctgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 attttcctta agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaactac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcata
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggttag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgaca
     2581 cctccaaagg gtaaagccga taaagcagtg gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg aaagaggtta
     2701 aaagcagatg gggatggttg gattgtacct cccgcagccg cgccgtggga cttcggattg
     2761 attgtgctac gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ccgccgcatt aaaagcggca ggtcgtaaag tgactcaggc aggataccct
     2881 gaagatcatc tcgatacgtt gtacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg tcttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttaa cggcctgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg tgccatggtt cacctccagt tcaggtttac gcgctttacg
     3541 ggtacctgat tttatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ctacgcctaa ggcgattttt ttaacggcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaaa ccgccatttc cctcactgac
     3781 gctcgcccat ttcatggaca gcgtaccggt aatttctgta gcaatagcca gacctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgcttag cgcaagcttt ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
     4261 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
     4381 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     4441 cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggctatggtg
     4501 ttgctgttag cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat
     4561 cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt
     4621 gacgtctcag ttgaccagct ggcgcattac attgatccga acgcggcgat gacgttgctc
     4681 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg
     4741 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
     4801 cgtccggttg aagggatggc ggcgattcag cgtgcgattg acagtgtgtc tcattatctg
     4861 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg catgggcgat gctcgccgcc
     4921 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct
     4981 aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc
     5041 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     5101 attctggagc cgcgcatcat gggacgtggg ctggggcttt cgacattggt ggtatttttg
     5161 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg
     5221 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     5281 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
     5341 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
     5401 gaacaacggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     5461 gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc
     5521 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     5581 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     5641 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
     5701 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
     5761 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
     5821 tgtctcttct gcggtgattt cgcggtgctc acccacttcc tgatcatcgc cagtagaaga
     5881 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     5941 cataatgtaa gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag
     6001 gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     6061 tgacaccacc actggcatat cagcaccacc gatggaggcg actaaatgcc agccgaatac
     6121 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tgtctgtgcg
     6181 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttatgacg
     6241 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac
     6301 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
     6361 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag
     6421 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
     6481 ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat
     6541 ccagccaacg ttacccgtat ccggtccaaa aatggtggcg attagcgcaa tcgccatccc
     6601 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagcc cggccagact
     6661 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     6721 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     6781 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     6841 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     6901 tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg acaacgcagg
     6961 ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccaac
     7021 cgaaaaggat gattgccagc gccatcaacg cgtatttacg ccacggtgag caggcacatt
     7081 tttcctcagt tttcacttcc ggtgccgctt tttgtgtcgc ctgcggctga gctgatacct
     7141 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
     7201 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
     7261 gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     7321 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     7381 attcacagtt gccaccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
     7441 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     7501 tgacaatgat atcgacctct tttgcctgag cggcaaagag ttccatttcc gctttgatga
     7561 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
     7621 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     7681 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac
     7741 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagtg atttgcccgg
     7801 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg
     7861 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
     7921 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     7981 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     8041 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttctgcgc
     8101 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     8161 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     8221 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     8281 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
     8341 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     8401 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     8461 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt
     8521 cgggtaaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     8581 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
     8641 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     8701 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     8761 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     8821 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     8881 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     8941 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
     9001 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     9061 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     9121 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     9181 agccagaagt caatgatgcc attaccaaag cggcaaaagc gaaaggtgcc tactctttct
     9241 acatcgttcg tcaaatcgat gccaaccaag gcggcaacca gcgtattact gcattcatct
     9301 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     9361 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccgg
     9421 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
     9481 caaaaggcgg gcgttacacc gtcacgctcc cggatggcac taaagtcgaa gaactgaaca
     9541 aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     9601 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     9661 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
     9721 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
     9781 cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     9841 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     9901 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     9961 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    10021 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
    10081 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    10141 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgtgcagt
    10201 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    10261 gccggaattg cgcctgtttg gcgatggtaa tacctggcaa tcgatcgtcg gtgcatcggc
    10321 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
    10381 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
    10441 gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacctgt
    10501 ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    10561 tgccgtggtt gtttctgccc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    10621 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggtgtggt
    10681 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
    10741 gatgggaccg tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
    10801 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc
    10861 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cactgtggtt
    10921 gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
    10981 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    11041 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    11101 tggcttatgg ttattgtacg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    11161 tgcaccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact
    11221 gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
    11281 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
    11341 aacccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
    11401 atggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacac actatccagc
    11461 cattgatgga ctgattaatg caggtgcgca gtgtattcag gctgattttt cgaccaacga
    11521 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
    11581 gcataacgcc agtgcgtgga tggcggaaaa accgggtgcg ccactggccg acgtactggc
    11641 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
    11701 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    11761 gcgcggtagc gacaaacata ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
    11821 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    11881 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
    11941 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    12001 ctgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
    12061 cttaatccag catataatca acacccatgc gcttatcccc cagcacgcaa cagaaccaac
    12121 aaaagcagcg ggcaagcgca tcattccggt gaaataccat agcgacacca ggtaaacaaa
    12181 atagggaata atcgaccaca tgctaaaaat gatggttgcg cgcaaggctt caatgccacg
    12241 ttcgctggca acaatataat gcgcgatgag cgcaaaggtc gggaaaagtg gaatcagccc
    12301 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    12361 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    12421 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    12481 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    12541 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
    12601 ccgtagagcc gcgcggcgac caggccgaag aaaccatttt gcgagaaaat ccggatttgg
    12661 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    12721 caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    12781 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgt
    12841 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacgctgacc aaaggtcttc
    12901 aggaaacgtc actgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccta
    12961 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    13021 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    13081 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    13141 taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact gtgcgtaaca
    13201 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
    13261 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ctggtgtaca
    13321 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    13381 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
    13441 agatggattt aaatctctct ttcgatctgc gtgtcgagcc actgagtaaa taccatcttg
    13501 atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    13561 cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    13621 tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt
    13681 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    13741 aagcagcggc gcaacgattt ggcgatgggc atctcaatga acgtatccac tttgatgagg
    13801 gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct
    13861 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    13921 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    13981 tgaatcgcga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    14041 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctatcaa
    14101 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctca
    14161 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    14221 tcaataacgc cctgcgctac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
    14281 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
    14341 atatctttga accttttgtg cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    14401 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    14461 acaccagcga actgggtggt gcccgcttct cgtttagctg gccgttatgg cataacatcc
    14521 cgcaatttac ctctgcttga cactacgcgc acggtggtca agtcaccacc actgtgctat
    14581 aaattaagga tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    14641 gactcaacac tacttttcgc cagatggaac aagagctggc tgcatttgcc gctcatcttg
    14701 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    14761 agcataatcc gcttaatcgt attgaggtga aacaacatct cggcaacgac gcgcagtcgc
    14821 tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    14881 aagccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    14941 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    15001 cggttgagtc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
    15061 ggctgatcac ccttaatgct taccgctcgc tcaccgttct gcacgacccc gccactttac
    15121 gttttggctg ggctaataaa catatcatta aaaatttgca tcgtgatgaa gtcctggcac
    15181 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    15241 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa
    15301 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    15361 aacaagtgca acacgcctgc cctacaccgc tgattgcact gattaatcgg gataatggtg
    15421 cgggcgtgcc ggacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
    15481 ataaacctca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    15541 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    15601 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    15661 gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    15721 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
    15781 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    15841 aattgtggat caccattgga cggaagacgt tcagttcaaa gttaccggaa gcgcccccca
    15901 tgttgatcgc cacgtcgttc cccatcacct gacagcagag catggttaat gcctcacact
    15961 gtgttgggtt cactttcccc ggcatgattg agctgcctgg ctcattttcc gggattgaga
    16021 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca
    16081 tcagtgacgc agccaaccct ttcaacgcac cgtgcgcctg aaccagggca tcacaggtcg
    16141 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    16201 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    16261 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
    16321 cgagcatcgc tacccagccg gaaatctcct gccccagtgt tagcggcgtg gcgtcctgca
    16381 agtgggttcg accgattttt acgatatcgg caaatgcacg cgatttttca ctcagcgtct
    16441 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
    16501 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    16561 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
    16621 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    16681 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    16741 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    16801 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    16861 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
    16921 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgattaattt tttctttctg
    16981 ttttgctttc gttaagcaac ttttagctca cttattattt accatttgat aacaaatgtt
    17041 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    17101 cgggtgcatt gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    17161 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
    17221 tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg
    17281 atactgccaa ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    17341 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
    17401 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    17461 gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta
    17521 tccatccgct ctttcagttt ggcgtgagca atatcacgac caacgataat cgtgccgtta
    17581 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    17641 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tgcgcagctc ttccgggata
    17701 tatttgcctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    17761 ttgatattac ggtcagcaga gcaggagacg cccataccga ccgggcagga tgcgccgtga
    17821 cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
    17881 agaccaagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaacgcc
    17941 tgaccgtgct cattcccttc cgttggcagt tcatcatagt atttcgcgga agccagtttc
    18001 accgttttaa ggttcgtttc tgcagaagtt ccaccaataa cgaacgcaat atgatacgga
    18061 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca agtaattttt cagtttcccc
    18121 ggcgtcagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    18181 ttggcgatac agaggaattt gtattcgtcg ccatcaacgg cataaagatc gatctgcgct
    18241 ggcagattgg tgccggtatt cacttcttta tacatatcca gcggcgcgtt ttgcgagtag
    18301 cgcagattat cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacca
    18361 ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcggtgcc ggtatcctga
    18421 caggttggca gaacgccttt cgccgcgatg tcggagttac gcaggaattg cagcgccaca
    18481 tatttatcat tttcgctggc ctccgggtca cgcagaatgt cggccacctg ttgttggtga
    18541 gccggacgca gcataaacga cgcatcatga aacgcctgac gcgccaacag agttaacgct
    18601 tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    18661 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    18721 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    18781 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    18841 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagcgg
    18901 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    18961 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccatcca
    19021 gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca cgagtgcaga
    19081 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa tcgactctgc
    19141 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    19201 cagcacagcc gctctccatt caggttcatc caaacaaaca caattctgaa atcggttttg
    19261 ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta
    19321 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    19381 aattttccga gattgtctcc ctactccagc ccgtcgcagg tgcacatccg gcaattgctc
    19441 actttttaca acagccacat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    19501 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggccctc gatagccagc
    19561 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggaa gacagcggtc
    19621 tgttctcccc actattgctg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    19681 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcaaactccg
    19741 ataacgtgct gcgtgcgggt ctgacgccta aatacattga tattccggaa ctggttgcca
    19801 atgtgaagtt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggcg
    19861 cagaactgga cttcccgatt cctgtggatg attttgcctt ctcgctgcat gaccttagtg
    19921 ataaagaaac caccattagc cagcagagtg ccgccatttt gttctgtgtc gaaggcgatg
    19981 caacgctgtg taaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    20041 ccgccaacga atcaccggtg actgttaaag gccacggtcg tttagcgcgt gtttacaaca
    20101 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    20161 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    20221 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    20281 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    20341 aaactgaccg ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    20401 ttcagcagtc agctgcaact gttggtgaaa cccattgccg ggaaagaaaa tccgtggatt
    20461 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccctttgcc
    20521 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggtcaat
    20581 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    20641 tcgcgcattg gttacagcgg tgattccagt tccgatattt cgctcaagcc actgaattac
    20701 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgctgacaga
    20761 gacggcaaag ctatctccct ttccggggag gcgcaaagtg gtcggataga cgcagttaac
    20821 gaatacaacc agaaagtgca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    20881 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    20941 tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    21001 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    21061 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca aattgatggc
    21121 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    21181 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    21241 gccctgccgc tgatgctgaa aggcgatccg gtgattactg tcgcgccgat aagctggaaa
    21301 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    21361 aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt cggttaaatc tctggatgcg
    21421 aaactgacca ttccggtgga tatggcaact gagttgatga ctcaggtagc gaagctggaa
    21481 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    21541 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcag
    21601 tatgctaacg gtcagatcac gttaaacgga cagaaaatgc cactggaaga tttcgttggt
    21661 atgtttgcaa tgccggcatt aaacgttccg gtcgtacccg ctattccaca gcagtaattc
    21721 acaaccaaaa cgccggagat ttttctctcc ggcgttattt cttacttcag cataaagtca
    21781 tactgaatac tgaatactga atactcattt tcacatccca gtaattcagg cgatcgccca
    21841 gcgcactgcc atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa ttcatccctt
    21901 gtaacggccc ttcgctgacc cgatagttca taccacctaa gatacccgtg ccttcaatcc
    21961 gcacatctcg tttactggat tcatagccgc gagtcacaat cggtgcaaat gtcagcgtaa
    22021 attgcggcgt taaacgatag ttgacgccca attgccagga ctgcattcct tcacggttac
    22081 gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca ccattcattg
    22141 catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc caggtcaatt
    22201 gtccactaac caacgccgtt tcattgggct ggctggtatt acgacttaaa ccttcgagtt
    22261 gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcaagc ggtatgcttt
    22321 tctcgagaat actttgtatc tgattggaaa catctttctg taccgatgcc cccagagaat
    22381 agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt
    22441 attttcccgt ctggctgtta tacacccctt catcacgaat accggttgtt gaatcagtcc
    22501 ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggcgtt aaagcccaat
    22561 aacctaatgc tccttcagaa actgtcggta atgtacgttg cgaactacca gccattaacg
    22621 gaatacgata aggtaagcta ccataactat aatccgtccc gaagcgacct aatttaagca
    22681 ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa cttttatcac
    22741 catcaagata ccaccgggta ctcatatcag cacgagcacc taatttataa acataatacg
    22801 ctccgccttc aacgccaata atattggaat aataaccact attgaaatcg agcaatcctc
    22861 cctgtaccca tgcgtaaata tctcttcccg cgccagcact gggcttatca gccctgcgca
    22921 attcgttttt cagtctgatg cgtagtcccg cttcatcgtc agccagttgc gccgcgtaag
    22981 cagaggtaag gccagaggca gccgtcaggc aaataacggc catggccact atttttctca
    23041 tagttgcacc tttgatgttc tgattatttt tattgaatat taattagtga tatcgctgat
    23101 taattgctgc tgcacttttt tacgattatc aatttcaacc acgatttcat tgaatttttt
    23161 atccgtgagc ggataaaacc agataataac gaatgcctgt agcataaatc cgcaaggtac
    23221 taaggcaatt gatgtgcgga tgcccataat aacttccggc gtttgcacct gattggcgat
    23281 atatccgctt aaccccaaaa taaaggcagg aattgaacct ccgattgcct gaccgcattt
    23341 acgggtaaat gagaatagtg aataggtgag cccttcaatt cgcacgccgg tcaggtattc
    23401 accgtattct acggtatcag cttccagcgc ccacatcacg gtcatggtaa cgccctgacc
    23461 aattgaagcg atggccaacg caaccaacgc caccggcagt gaccagacgg aaacccagaa
    23521 gaatagcaga taaccgcagg ttcccagcaa agcgccaatc aggaaggtat tctttttacc
    23581 gatcctcgcg accatccccg gcaccagcgg tgccgatgcc acagtaccga ccaggttttg
    23641 caccagtacc agcacagtga acagcccggt atcatttaac acatagcgca catagaacaa
    23701 cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac agcgcaccga tgcacaacat
    23761 aaacagcggg cgattccgtt tcagggtttg cagactgatc ttcaatgacg gctgcgccac
    23821 gatacgtacc acattctcac gcgtcgattt gaagcagatg aagtaaagca ccattccggc
    23881 aatcgccagc acaatcgtcc agaaatggta taccgacacc atctcttccg ggctggagtt
    23941 cttaatgctc ggccctatca gaaatgccag gcagacaaag gtcaatgaag cggcaatccc
    24001 acgagccgcg cccagacggg cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga
    24061 accataagga atattcacca ggctgtagca aagcccgagg cccatgtagg tcaaatatgc
    24121 atacactact ttgctgctat ggctccagtc ggtcggtacc cagaatacca gcacgctgaa
    24181 gatcattaac ggcgcagtac cgaagagtaa aaaggggcgg aattttcccc agcgggtatt
    24241 cacactgtcc accactcgtc cggcaaagac gtcggcgaag gcatcgaata cccgcaccag
    24301 taacagcatg gtgcctgccg cagcggcacc gacgccagcg acgtcggtgt agtaactcaa
    24361 caggaagagc gcccccattg cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc
    24421 gacgatggtg cgccaggaga gttgttgatt cattgtttgc ctccctgctg cggtttttca
    24481 ccgaagttca tgccagtcca gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg
    24541 tcgcgggtga agatcccctt cttgttaccg ccaacgcgca atatgccttg cgaggtcgca
    24601 aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg
    24661 tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt gtacatcgag
    24721 tgcaggccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg atgcagtttc
    24781 tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa atcgccgctt
    24841 tggacatacc aaccgtaata acggttcagg cacagcacat caaagagatc gctgatggta
    24901 tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt cgggtcgagt
    24961 ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc
    25021 ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg cgcaatcagc
    25081 tctttaatcg cctgtaagtg cgcctgctga gttttcccgt tgactgcctc ttcgctgtac
    25141 agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagaggggtt aaagccgaca
    25201 gctgcagttt catcaattac cacgatgcca tgttcatctg cccagtcgag catctcttcg
    25261 gcataagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat taatgcgtga
    25321 tcgtgcacca tcagcacgtt atcgaatcct ttgccgcgca aatccgcatc ttcatgacgg
    25381 ccaaagccag taaagtagaa cggtttgtgg ttgatcagga actgttcgcc cttcactgcc
    25441 actgaccgga tgccgacgcg cagcgggtag atatcacact ctgtctggct tttggctgtg
    25501 acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt caccacttgc
    25561 aaagtcccgc tggtgccttg tccagttgca accacctgtt gatccgcatc acgcagttca
    25621 acgctgacat caccattggc caccacctgc cagtcaacag acgcgtggtt acaggcttgc
    25681 gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt
    25741 acgctgcgat ggatcccggc gtagttaaag aaatcatgga agtaagactg ctttttcttg
    25801 ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag ttcgttgttc
    25861 acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac atcggcttca
    25921 aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca cactttgccg
    25981 taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc tttcggtata
    26041 aagacttcgc gctgatacca gacgttgccc acataattac gaatatctgc atcggcgaac
    26101 tgatcgttaa aactgcctgg cacagcaatt gcccggcttt cttgtaacgc gctttcccac
    26161 caacgctgat caattccaca gttttcgcga tccagactga atgcccacag gccgtcgagt
    26221 tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggagtc ctcattaaga
    26281 taaaaatact ggtcaacctt taatctgatt agttaaccaa ttctgtgttg cacacagtta
    26341 tctgtgagaa acccatggaa gagaaatgga aataaaaaga actgacaccg cgatcaaaaa
    26401 accaaaaact gcaataccca cagcgtgata tagatcgcat taatctttaa aacggcgttg
    26461 acagaagcca taaaataagc aaccgaacgt tcgctcgagc atattgggaa cgataaactc
    26521 tcatgacata tggctacagt gaatattttg gggaggaatt ccccgctccg gcgagattgt
    26581 attttgtaga gggaaattaa agtatgaaac gcgggctcag ttgtcaccag cccgcggaaa
    26641 gagagagaga atcaggatgc ggttaagata ccgccaatca tcgcgcgtaa tccctgagca
    26701 aacgacgctt cacgcgccag atcttccgct tcgatatcgg ccagtacacc gtaggtcaga
    26761 tccagcagta aacgtgaagt cattgccgtg ttgatgtctg ggcttatctc gcctttttgc
    26821 tgggcatcac gcatccgctg agcaacaaat tcagtgatcg tcatatgctt atttttaagc
    26881 atggcggcga cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt
    26941 tgcccaaagg cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga
    27001 atcccttcaa tcggttcccg gaaacgggcc agcgccctct cctggtcctg taagataatc
    27061 gcctgaatca aggcttcttt ggagatgaaa tggtgataga gcgtcccggg actaatggcg
    27121 caagatttac agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct
    27181 ctggcagcat tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc
    27241 attaaaccat tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat
    27301 cattgctgtt gaatagtgcg aaggcacact ctattcgtat gcttttcatc cagctattgg
    27361 ttaaccaata gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca
    27421 cgctatcccg gtaaacaaca catacggctg tttaccggtt cgttagtgta ttaattgagc
    27481 tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc
    27541 gagcaaagga acaacgctgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc
    27601 gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt
    27661 aatattgccc ccggcgcaat gccatttacc cgaatatttt tttcacccag gtcaaacgcc
    27721 atatttctga ccagatgact ggccgcagct ttagatgatg cataggaagt catgtttata
    27781 tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc
    27841 atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca
    27901 cggcgaaaat ctgccattgg catatcaaac ggtttaggac caccgccacc ggcgttgtta
    27961 accaggatat caactttacc cagcttactg acggcaaagt ctgccagtgc agagagttcc
    28021 tgttcggaag taatatcaca acgccaggca aatgcctgac cacccagttg ttgaatttca
    28081 tctacaacat ggttagctgc gtcggcatta atatcactga ccactacaga tgcgccagct
    28141 gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt gatgatggcg
    28201 cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag
    28261 taaaaataaa aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggac
    28321 actaccaggt atagcagata aagagaggat tatttcgccg caatgagccg ggcgggaata
    28381 ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt
    28441 gtggtaccaa gttcccgcgc aggcgtgctg gcccaggtaa cgggtatatc atcaagtgtg
    28501 gttggtgtcg catcggtaaa tgccgccagc gaaacctgtt gctcaaagta acgatcgact
    28561 ccgctttccc cgctttgccg ccctgctttc agcaaaccaa accatgcccc catcgcaata
    28621 gtttcgttat agcaaaccac ggcactgatg gtcgggttat gacgtaaaag cgccgtgata
    28681 gcttccgcgg cttgcttctg gctggaagtg cactccaaca cccaatcgct gtgaaacgga
    28741 aggccaaatt ttagtagagt tgcacaatag ccccccaccc gttctgcacg ggttaatgag
    28801 gaactttgcc ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag atgctccgtc
    28861 aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacatcatc aagataactg
    28921 gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg
    28981 ctgcttcctg cagccccggc aattaccaca ccatcgacac cctgattcag taacagtgaa
    29041 aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc
    29101 tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg cgcagataaa
    29161 tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctggcga
    29221 ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag
    29281 attcgccctt tgccactaag caccagcgaa acggtactta ccgacacgcc cgcagccagc
    29341 gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt
    29401 atgtgacaga taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta
    29461 atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg
    29521 aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca
    29581 cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt
    29641 cgttctgcgg cattatgctc ggcattggta gttctcttag cagccatgat gtcataaccc
    29701 tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg
    29761 gctcgtttgc ttttagtttc ctgcctgtca tgttctgtat cgccatcccg ctgggcctgg
    29821 cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggatatgcg gtaatgaacc
    29881 tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacagac gccgccgttc
    29941 tgaaagccaa taacatccag agcattcttg ggatccagtc gatcgacacc gggatcctcg
    30001 gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgattccat aatatccgcc
    30061 tgccggatgc gctggcattc ttcggcggta cgcgcttcgt accaattatc tcctcgctgg
    30121 tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcc atgggtatta
    30181 gcggcttagg ccatatgatc aatagcgcgg gtgatttcgg accgatgctg tttggtaccg
    30241 gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta
    30301 ccgatgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct
    30361 tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc
    30421 tttcgcaagg caaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg ttagctatgt
    30481 atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga
    30541 tcgcctgtgt cgttggcggc acgaccgaac cgctggaatt cctgttcctg ttcgtagcgc
    30601 cagttctgta tgttatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc
    30661 ttggcgtcac catcggtaat accgacggca atatcatcga ctttgtggtg ttcggtattt
    30721 tgcacggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg
    30781 tttactacat catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg
    30841 atagcgaagt ggccagttca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt
    30901 acaacgttcc ggcaatcctc gaagccttag gcggtgccga caatattgtc agcctcgata
    30961 actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac
    31021 tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta
    31081 tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg
    31141 cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta
    31201 cgcagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt
    31261 cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc
    31321 acggcgtatt tggctacagc cgctggaaaa acgatgaatt tctcgcggct attgcccact
    31381 ggttttccac ccagcattac accgctatcg atcctcagtc ggtggtgtat ggcccttctg
    31441 ttatctatat ggtctcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga
    31501 tccacacacc cgcctatgac gcattttaca aggccattga aggtaatcag cgcacagtaa
    31561 tgcccgttgc tttagagaag caggctgacg gctggttttg cgatatgagc aagctggaag
    31621 ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagtccacag aatcctaccg
    31681 ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctgtgcgag cgtcatggtg
    31741 tgcgggttat ttccgatgaa atccatatgg atatggtttg gggcgagcag ccgcatattc
    31801 cctggagtaa tgtgggtcgc ggagactggg cgttgctaac gtcgggatcg aaaagtttca
    31861 atattcccgc cctgaccggt gcttacggga ttatagaaaa tagcagtagc cgcgatgcct
    31921 atttatcggc actgaaaggc cgtgatgggc tttcttcccc ttcggtactg gcgttaactg
    31981 cccatatcgc cgcctatcag caaggtgcac cgtggctgga tgccttacgc gtctatctga
    32041 aagataacct gacgtatatc gcagataaaa tgaacgccgc gtttcctgaa ctcaactggc
    32101 agatcccgca atccacttat ctggcctggc ttgatttacg tccgttgaat attgacgaca
    32161 acgcgttgca aaaagcactt atcgaacaag aaaaagtcgc gatcatgccg gggtatacct
    32221 acggtgaaga aggtcgtggt tttgtccgac tcaatgccgg ctgcccacgt tcgaaactgg
    32281 aaaaaggtgt ggctggatta attaacgcca tccgcgctgt tcgttaaccc caattgcgca
    32341 acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg
    32401 cacttttata ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat
    32461 atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag
    32521 tataatatct cgcttcctgc acaatccctg gaaacattga ttccccacgt tcaggtcatt
    32581 gccaacgaac ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc
    32641 gcctctcttg atgcctgccg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac
    32701 ggcctgcact atgtcgagct gcgtttttca ccaggctaca tggcaatggc acataagctg
    32761 cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt
    32821 ggtgtgcagg cgaagcttat cggcattatg agccgaacct tcggcgaagc cgcctgtcag
    32881 caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt
    32941 gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccttgc gcgtgatgcg
    33001 ggctggcata ttaccgtcca tgcaggcgaa gccgctgggc cggaaagcat ctggcaggcg
    33061 attcgtgaac tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggtg
    33121 ctgatggatt ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt
    33181 cagaccagca ccgtagcaga gctggctgca catccgctga aaacgttcct tgagcatggc
    33241 attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa
    33301 tataccgttg ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca ggcacagatt
    33361 aatggtcttg aaatggcttt cctcagcgca gaggaaaaac gcgcactgcg agaaaaagtc
    33421 gctgcgaagt aacaaaaatg gatggtgcaa attgcaccat ccatttttca cgcaaggcac
    33481 aaagtcgcgc gatgtttggc ggattcgatg cccagctcaa tcaactccat tacctggatt
    33541 gcctggcttg ccggaactgg attttcacca tcgccattta acgcatcacg aatcgccgca
    33601 tagtaagccg gataattccc aggcaccgtc aacagcgttt cttcgacacg ttcctcacct
    33661 tccacgcggg taagtacgcc atcacgcata tcgtagcccc agtcttcctg cggcagacgc
    33721 tcgccatttt tcagacgttc ttcctgtgga tcgaggccat atttcacata actgcctcgg
    33781 gatccatgca cgatataccg tgctgactca gcggctgcca gcatggtacc gtgtaaaatg
    33841 actcgccgct gtggatagga caagatggcg tggaaataat cggtcgactg cgctccaggc
    33901 cgtaactgtg ccaagtccac cgtcatgctg accggtaaac caaataatgt aatggcctga
    33961 tcaagaagat gcggtgctaa atcgtaccag ataccactgc ctgggccacc ctgttcacgc
    34021 caacgatcgc gcacctgcgg acggaagcgg tcaaaatgag actcaaagta agcgacttca
    34081 cccagcacgc cttccgcgag taaacctttt agcgtcaaga aatcgctatc ccagcgacgg
    34141 ttatggaata cagacagcac acgccccagg ctttttgcca gcgcatccag ctctcgcgct
    34201 tgtgacagtg tcacggtaaa gggtttatca acgaccacat gtttacccgc ctcaagcgct
    34261 gctttggcta acgggaaatg ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg
    34321 ggatcgttaa acagatgctt cggctcagag acaaccgtaa ccgttggcca gtcggctttt
    34381 acttttgttt catcactgct ggagattacc gccagttcca gcccgggcgt gcccgcaatc
    34441 aggggcgcat ggaaggtttt gctcgcataa ccacacccaa tcaacccaac acggatgttg
    34501 tcgctcatgt cattgcctct cattgaagta tgatggctat ttgacactat cctttaccct
    34561 cgctcaacag tttaataacc tgccagcaat aagggatgtt gtttaactta agtcaaaaaa
    34621 atagcgattt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct
    34681 ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat caacagagat
    34741 gtgagttgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg tcgtttcagt
    34801 cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac agagtactac
    34861 ggtacaacac aagtaagctc tgcacttgta gagcgacatg ctgcccgtcc gggtgcatgt
    34921 tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg ttggtgattg
    34981 tcagggatag taaagcgcgt tactcttttc agagcagttt ttcatccctg aatatttgcc
    35041 atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa attttatcat
    35101 cactaataat acctacccct ttggtattaa ttattttatg gaccttttat gactgttcag
    35161 gactacttat taaaatttcg caaaatcaat tcactcgaaa gtctggaaaa actctacgac
    35221 catcttaatt acaccctgac ggacgatcag gaactgataa atatgtatcg tgctgccgat
    35281 caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca ggtaccgaag
    35341 tccgtctggc actatgttca ataaagaaag tagcgatcgg ttataaaagc cttataataa
    35401 cgcccctaaa attagatttg cctgtttgcg tcgctattct ggagaaccta tgaccaccac
    35461 aacgccacaa cgtattggag gctggttgct tggcccgttg gcctggcttt tagtcgctct
    35521 gttaagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc cgcaaacatt
    35581 tcaaacgctc ggcgcacaag cactgacgac acaaatctta tggggcgttt cttttattac
    35641 cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttta aacgtcggcg
    35701 ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg cagtaaaagc
    35761 gttcgctttt tcacccgtgg aagatggcat agccgttcgc cagttgctgt ttactttgtt
    35821 ggcaaccgca ttgatcgtcc cctatttcaa acgctcgtcg cgggtgaaag cgacgtttgt
    35881 gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa cggataatag
    35941 gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt gtcggaactg
    36001 tactggtcaa taactttgta ctggtcaagt ttctcggtct ctgtccgttt atgggggttt
    36061 ccaaaaagct ggaaaccgcg atgggcatgg ggctggcaac aacgtttgtg atgacgctgg
    36121 cgtctatttg cgcctggctt atcgatacgt ggattttgat cccacttaat ctgatttacc
    36181 tgcgcaccct ggcatttatt ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg
    36241 tgcgcaaaac cagcccggtg ctttaccgtt tgctggggat ttttttgccg cttatcacca
    36301 ccaactgtgc cgtgctcggc gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc
    36361 agtcggcgct gtacggtttt tccgccgctg tcggtttttc gctggtgatg gtgctcttcg
    36421 cagccatccg cgaacgcctt gctgtggctg atgtcccggc tccttttcgc ggtaatgcca
    36481 ttgcgttaat taccgcaggt cttatgtctc tggcctttat gggctttagt ggtttggtga
    36541 agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt
    36601 tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc cggtcgttga
    36661 aaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg
    36721 cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc aaccgttgcg cccctggtgg
    36781 cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gaaccgcagc cgctggatgg
    36841 cgaagcgcaa gagctaacgc ctgcgcggat ggtggcggtt attgatgaaa ataactgtat
    36901 tggctgcact aaatgtattc aggcgtgtcc ggtagacgcc atcgttggcg ctacccgagc
    36961 catgcatacg gtaatgagtg aactctgtac gggctgcaat ttatgtgttg atccgtgccc
    37021 gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga aatgggatct
    37081 gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta ttctctgcat
    37141 tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag atgaaaaccc
    37201 agtccaacgg tacccccctg cgccaggtac ccctggcgca gcgttttgtt attccactga
    37261 aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg
    37321 gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta
    37381 ccgttacggc tattgcgccc cactctacgg ctcatccttc agctttagct gaattaagcg
    37441 tgattattga tgccgatggt gaagactgct ggatcccacg agacggctgg gccgattatc
    37501 gctctcgccg tcgcgaagag ttaatcgagc gcattcatca gtttggtgtt gccgggctgg
    37561 gcggtgcggg attcccgaca ggcgttaaat tgcagggcgg cggagataag attgagacgt
    37621 tgattatcaa cgctgctgag tgcgagccgt acattaccgc tgatgaccgt ttaatgcagg
    37681 attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg cagccacgcg
    37741 aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc
    37801 tggcggactc tcacgatatt tctctgcggg tgattccaac caaatatcct tctggcggtg
    37861 ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg cgttcatccg
    37921 atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt gccgttattg
    37981 atggcgagcc gattaccgag cgtgtcgtaa ccctgactgg cgaagcaatc gctcgcccgg
    38041 gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgac gccggattct
    38101 gcccctctgc cgatcaaatg gtgattatgg gcggcccgct aatgggcttt accttgccat
    38161 ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct gccaatgagc
    38221 ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct gacgcctgcc
    38281 ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa cacgataaag
    38341 ctaccacgca taacattgcc gactgcattg aatgtggggc ttgcgcgtgg gtttgcccga
    38401 gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc
    38461 aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg
    38521 agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa cctgcagcca
    38581 aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag gcccaggcta
    38641 cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt attgcagcac
    38701 gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa actaacgacg
    38761 cagcaaccgt tactgatcca cgtaaaactg ccgttgaagc agctatcgcc cgcgccaaag
    38821 cacgcaagct ggaacagcaa caggcaaatg cggaacctga acaacaggtc gatccgcgca
    38881 aagctgccgt cgaagccgct attgcccgtg ccaaaacacg caagctggaa cagcaacagg
    38941 ctaatgcgga acc
//
LOCUS       DAZUGK010000020        38434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZUGK010000020 DAZUGK010000000
VERSION     DAZUGK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.020"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..527
                     /locus_tag="ACHY5W_000918"
     CDS             <1..527
                     /locus_tag="ACHY5W_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445858.1"
                     /translation="LTGDTEFLDSAIIGPVGNSIKCYYVSSLSGFYYTPAYVFADVEF
                     SMRQYIKCSHLTNKYGDDNLMYVFCKGGYEFDVNYLCVMASDLDNIKNTGDFPKITED
                     SPKASSKRNEVIYSLIKLIPEMGDVDLDTESLPKIADLIDSIAASRGIEFPKTHWQTW
                     QKYLGRESQQKRKK"
     gene            619..843
                     /locus_tag="ACHY5W_000919"
     CDS             619..843
                     /locus_tag="ACHY5W_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4445859.1"
                     /translation="MNQNTQFQSLSDRVIREDECRKLTGVCRTTRYELEKKGRFPSRL
                     NLGGRSVGWLLSEVMEWVKSRDRINSGKAV"
     gene            complement(982..1164)
                     /locus_tag="ACHY5W_000920"
     CDS             complement(982..1164)
                     /locus_tag="ACHY5W_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4445860.1"
                     /translation="MQEKNEKPKFDRSGSTKKNIRFEDTLLQQINEVAGPGNFSSWVK
                     NACRDKLRKEGIEPKG"
     gene            1310..1513
                     /locus_tag="ACHY5W_000921"
     CDS             1310..1513
                     /locus_tag="ACHY5W_000921"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4445861.1"
                     /translation="MLNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPVGCVMVFAARIRQGVCRA"
     gene            1506..1688
                     /locus_tag="ACHY5W_000922"
     CDS             1506..1688
                     /locus_tag="ACHY5W_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445862.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAVNMQAAIDSVISNSGKA
                     GLSHVIIAAVHELKGV"
     gene            1692..1883
                     /locus_tag="ACHY5W_000923"
     CDS             1692..1883
                     /locus_tag="ACHY5W_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445863.1"
                     /translation="MQEFTLNEITERAQQVEIICRLLEVYPSKLNDGDISALAGLLAK
                     LSGSVALWLIDERFKRYEQ"
     gene            1873..2115
                     /locus_tag="ACHY5W_000924"
     CDS             1873..2115
                     /locus_tag="ACHY5W_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445864.1"
                     /translation="MNNNKRVICELNELTQSAISYNNAVSEIINVWFESLTNCERDES
                     TTRLTFAISELLNISTSKIESISEKINGLSGHAIGE"
     gene            2121..2420
                     /locus_tag="ACHY5W_000925"
     CDS             2121..2420
                     /locus_tag="ACHY5W_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4445865.1"
                     /translation="MKLKYSGLTASGKAQPEFMRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVIPVYQFDRDFSLVQAVPRNVPTSREKARANIQKMKNMINGFRGKK"
     gene            2417..4171
                     /locus_tag="ACHY5W_000926"
     CDS             2417..4171
                     /locus_tag="ACHY5W_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFS4445866.1"
                     /translation="MKSAPNLKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEHDARIA
                     RDNEPPVWLGDQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHGQKVENWREYLARERQNLSEGLVVELPVKQKAQLSQMADSERAQLLSDRF
                     DGVCVHPESEIVHVWRCGLWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLDHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTTVIRAVVLATNNNPMIFTERAGGVSRRRVIFRFDNIVREDEKDKELPEKIAAE
                     IPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVAEFSKAMKPAAKVYGHEYITRRV
                     KGVTQTNAITTDDCDAFL"
     gene            4458..4709
                     /locus_tag="ACHY5W_000927"
     CDS             4458..4709
                     /locus_tag="ACHY5W_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445867.1"
                     /translation="MHTSGKLNNQKKPHNRAIDLTEHWLRVAIKIIDRHTREGYAKEN
                     PELISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            4840..5034
                     /locus_tag="ACHY5W_000928"
     CDS             4840..5034
                     /locus_tag="ACHY5W_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445868.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDVIRRMNEAGIYGRAATPT
                     IGHLARVLLRERLGIATGEA"
     gene            5038..5199
                     /locus_tag="ACHY5W_000929"
     CDS             5038..5199
                     /locus_tag="ACHY5W_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFS4445869.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSNEVE
                     GLISLLGEQ"
     gene            5328..5816
                     /locus_tag="ACHY5W_000930"
     CDS             5328..5816
                     /locus_tag="ACHY5W_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4445870.1"
                     /translation="MALTTKKKRFADALSRGANPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            5828..5989
                     /locus_tag="ACHY5W_000931"
     CDS             5828..5989
                     /locus_tag="ACHY5W_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445871.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            5979..6902
                     /locus_tag="ACHY5W_000932"
     CDS             5979..6902
                     /locus_tag="ACHY5W_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4445872.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEITKRNDPAVSKFLE
                     KSIINRMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLHSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIEIFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            7881..8201
                     /gene="clpS"
                     /locus_tag="ACHY5W_000933"
     CDS             7881..8201
                     /gene="clpS"
                     /locus_tag="ACHY5W_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFS4445873.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8232..10508
                     /gene="clpA"
                     /locus_tag="ACHY5W_000934"
     CDS             8232..10508
                     /gene="clpA"
                     /locus_tag="ACHY5W_000934"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFS4445874.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLADKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10854..10989)
                     /locus_tag="ACHY5W_000935"
     ncRNA           complement(10854..10989)
                     /ncRNA_class="other"
                     /locus_tag="ACHY5W_000935"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11033..11120)
                     /locus_tag="ACHY5W_000936"
     tRNA            complement(11033..11120)
                     /locus_tag="ACHY5W_000936"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11084..11086),aa:Ser,seq:gga)
     gene            complement(11374..11592)
                     /gene="infA"
                     /locus_tag="ACHY5W_000937"
     CDS             complement(11374..11592)
                     /gene="infA"
                     /locus_tag="ACHY5W_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4445875.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11877..12581)
                     /gene="aat"
                     /locus_tag="ACHY5W_000938"
     CDS             complement(11877..12581)
                     /gene="aat"
                     /locus_tag="ACHY5W_000938"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFS4445876.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDGLVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12623..14344)
                     /gene="cydC"
                     /locus_tag="ACHY5W_000939"
     CDS             complement(12623..14344)
                     /gene="cydC"
                     /locus_tag="ACHY5W_000939"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFS4445877.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14345..16111)
                     /gene="cydD"
                     /locus_tag="ACHY5W_000940"
     CDS             complement(14345..16111)
                     /gene="cydD"
                     /locus_tag="ACHY5W_000940"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFS4445878.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16234..17199)
                     /gene="trxB"
                     /locus_tag="ACHY5W_000941"
     CDS             complement(16234..17199)
                     /gene="trxB"
                     /locus_tag="ACHY5W_000941"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4445879.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVSGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17743..18237
                     /gene="lrp"
                     /locus_tag="ACHY5W_000942"
     CDS             17743..18237
                     /gene="lrp"
                     /locus_tag="ACHY5W_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFS4445880.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18372..22517
                     /gene="ftsK"
                     /locus_tag="ACHY5W_000943"
     CDS             18372..22517
                     /gene="ftsK"
                     /locus_tag="ACHY5W_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4445881.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPTTEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYVTAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQY
                     QQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            22672..23283
                     /gene="lolA"
                     /locus_tag="ACHY5W_000944"
     CDS             22672..23283
                     /gene="lolA"
                     /locus_tag="ACHY5W_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFS4445882.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            23294..24637
                     /gene="rarA"
                     /locus_tag="ACHY5W_000945"
                     /gene_synonym="mgsA"
     CDS             23294..24637
                     /gene="rarA"
                     /locus_tag="ACHY5W_000945"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFS4445883.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24728..26020
                     /gene="serS"
                     /locus_tag="ACHY5W_000946"
     CDS             24728..26020
                     /gene="serS"
                     /locus_tag="ACHY5W_000946"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4445884.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQDTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            26259..28703
                     /gene="dmsA"
                     /locus_tag="ACHY5W_000947"
     CDS             26259..28703
                     /gene="dmsA"
                     /locus_tag="ACHY5W_000947"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFS4445885.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28714..29331
                     /gene="dmsB"
                     /locus_tag="ACHY5W_000948"
     CDS             28714..29331
                     /gene="dmsB"
                     /locus_tag="ACHY5W_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFS4445886.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDDGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29333..30196
                     /gene="dmsC"
                     /locus_tag="ACHY5W_000949"
     CDS             29333..30196
                     /gene="dmsC"
                     /locus_tag="ACHY5W_000949"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFS4445887.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAETQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELVTIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(30232..30858)
                     /gene="ycaC"
                     /locus_tag="ACHY5W_000950"
     CDS             complement(30232..30858)
                     /gene="ycaC"
                     /locus_tag="ACHY5W_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFS4445888.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            31173..32321
                     /gene="ycaD"
                     /locus_tag="ACHY5W_000951"
     CDS             31173..32321
                     /gene="ycaD"
                     /locus_tag="ACHY5W_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4445889.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSLAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32531..33961
                     /gene="ycaM"
                     /locus_tag="ACHY5W_000952"
     CDS             32531..33961
                     /gene="ycaM"
                     /locus_tag="ACHY5W_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4445890.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVVMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33962..34072)
                     /locus_tag="ACHY5W_000953"
                     /pseudo
     CDS             complement(33962..34072)
                     /locus_tag="ACHY5W_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(34171..34911)
                     /gene="pflA"
                     /locus_tag="ACHY5W_000954"
     CDS             complement(34171..34911)
                     /gene="pflA"
                     /locus_tag="ACHY5W_000954"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFS4445891.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35103..37385)
                     /gene="pflB"
                     /locus_tag="ACHY5W_000955"
     CDS             complement(35103..37385)
                     /gene="pflB"
                     /locus_tag="ACHY5W_000955"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4445892.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37440..38297)
                     /gene="focA"
                     /locus_tag="ACHY5W_000956"
     CDS             complement(37440..38297)
                     /gene="focA"
                     /locus_tag="ACHY5W_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFS4445893.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9927 a         9213 c        10056 g         9238 t
ORIGIN      
        1 gtttaactgg tgacactgaa tttttagata gtgccattat tggtcctgta gggaattcta
       61 tcaaatgtta ttatgttagc agcttatcag ggttttatta tactccggca tatgtttttg
      121 ctgatgtcga atttagcatg agacaatata taaaatgttc tcatcttacg aataagtatg
      181 gtgatgataa tctcatgtat gttttttgta agggtggtta tgaatttgat gttaactatt
      241 tgtgtgtgat ggctagtgat ttagataata taaaaaatac gggagacttc ccgaagataa
      301 cagaagatag cccaaaagca tcgtcaaaaa ggaatgaggt tatttactct ttaattaaac
      361 ttattccaga aatgggtgat gtagacctgg acacggaatc attacctaaa atagcagatt
      421 taattgattc tatagctgca tccagaggga tagaatttcc aaaaacacat tggcagacat
      481 ggcaaaagta tttggggcgt gaatctcaac aaaaacgtaa aaagtgatat cacgtgctgg
      541 tagtgtgata tcacacaaac ggcgttttac taccgcctag aatcgtcctc gttcggcaac
      601 gttcgaggac gtttttttat gaatcaaaat acacaatttc aatcactatc agatcgcgta
      661 atccgtgagg atgaatgccg taagttaaca ggagtgtgcc gcactacacg ctacgaactg
      721 gagaaaaaag ggcgctttcc gtcacgtctt aatctgggcg gtcgttctgt agggtggctg
      781 ctgtccgagg ttatggaatg ggttaaaagc cgggatcgca ttaattcagg taaggcagtg
      841 taaaggggag catatgacac ataaaacaaa ggcgacctgt ggcggtcgcc aatgggaaaa
      901 cactaaacat aagcccgaac atcatagcga tttgctgtct ggtgggcaat gtgctcagtg
      961 tgctggcttg ctggtggact atcagccttt tggttctatg ccttctttgc gtagcttgtc
     1021 acgacaggca tttttaaccc atgaactgaa attaccaggc ccagcaactt cgttaatttg
     1081 ttgcaacaat gtgtcttcga agcggatgtt tttctttgtg ctgccagagc gatcaaattt
     1141 tggtttctca tttttttctt gcattggtac gtaccaccat tataaactga aacccaatgg
     1201 tacgtaccat attgaagata cgcaaccatt aaatacagcg aagccccgca gtggtggaac
     1261 actcgcaggg cgtctaacaa ccaacgatag caacagtatc gaggtagcta tgttaaatca
     1321 taccacacac ccgcaagggc gggactcgca caacctgaat aaatacatct ggcgttttat
     1381 cgccctgagc acggcacagc cgcgcgtgat taccatcgag gccaccagcg aacaggaagc
     1441 acgccagcaa tccccagttg gctgcgtgat ggtattcgcg gcccgtattc gtcagggggt
     1501 gtgccgtgct taaaaccttc cgcgtttttt cccgtgctgt taacaagcgc ggctataccg
     1561 ttggcatcac gcagaacgtg aaggccgtta atatgcaagc ggcgattgat tcagtaatca
     1621 gtaattccgg taaagcaggt ttatcacatg taattattgc agcagtacat gaattaaaag
     1681 gggtgtaatt aatgcaggag tttacattaa acgaaattac ggaaagagcg cagcaggttg
     1741 aaatcatttg ccgccttctt gaggtgtacc ctagtaaatt aaatgatggt gatatatccg
     1801 cattagctgg ccttctggca aaactttctg gaagtgttgc gctgtggtta attgatgaga
     1861 gatttaaacg ctatgaacaa taacaaacgc gtaatttgcg aattgaatga actcacacaa
     1921 agcgcaatat cttacaacaa tgccgtaagt gagattatta acgtctggtt tgaatcgctg
     1981 acaaattgcg agcgtgacga gtcaacgaca agattaacct ttgccatttc tgaattactg
     2041 aatatttcca ccagcaaaat agagagcata tcagagaaga ttaacggctt atcaggtcac
     2101 gctatcggag aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaag
     2161 ctcaacctga atttatgcgc ggggatattt accgcgacca gtacggcggc acggtaatga
     2221 ttaagggcgt ggaggaacgg cgcgtaactt accgccgtga aggctacgaa tatgattgcg
     2281 tgattcctgt ttatcagttc gaccgtgatt tttctctggt gcaggctgta ccgcgtaacg
     2341 tgcccaccag cagggagaaa gcacgcgcca atattcagaa gatgaaaaac atgattaacg
     2401 gattcagagg caagaaatga aaagcgcacc gaacttaaaa aaacaaccac gcggaataaa
     2461 acacaaagac acagaggtaa ttattttcgc gggtagtgat gcgtgggcac atgcaaaaca
     2521 gtggcaggaa catgacgcgc gtatagccag agataatgag cctcctgtgt ggcttgggga
     2581 tcagcagtta tccgaactgg ataagctgca aattgtgccg gaaggccgaa aatcagtacg
     2641 catattcagg gccggatatc ttgcgccagt aatgataaag gcgattggtc agaagctggc
     2701 ggcggcaggt gtacaggatg caaattttta ccccgaagga atgcacggtc agaaggtgga
     2761 gaactggcgc gaatatcttg cccgtgagcg ccagaatctt tctgaaggtc tggtcgttga
     2821 gcttccggta aagcaaaagg cgcaactttc gcagatggcg gacagtgaac gcgctcagct
     2881 gctttccgat cgctttgatg gcgtttgcgt acatcctgaa agtgaaatcg ttcacgtatg
     2941 gcgctgcggg ttatggtgtc cggtcagcac aatggaactt agccgcgaaa tggtggcgat
     3001 ctattcagag cacagggcca ctttcagcaa acgcgtgatc aataacgccg ttgaagcgtt
     3061 aaaagttatt gccgaaccaa tgggggagcc gtccggcgat ttgctgccgt tcgccaatgg
     3121 tgcgcttgac ctgaaaacgg gggaattttc cccgcacacg ccggagaact ggatcaccac
     3181 gcacaacggc attgagtaca cgccaccagc acctggggag aacattcgcg acaacgcgcc
     3241 aaactttcac aaatggcttg atcacgcagc cggaaaagac ccgcgcaaga tgatgcgtat
     3301 atgtgccgcg ctgtacatga ttatggcgaa ccggtacgac tggcagatgt ttattgaggc
     3361 caccggagac ggcggtagcg gtaaaagtac attcacgcac atagccagcc ttctggcagg
     3421 gaaacagaac acggtaagcg ctgaaatgac atcgcttgat gatgctggtg gacgtgcgca
     3481 ggttgtcggg agtcgtctta tcgtcctggc tgaccaaccg aaatatacag gcgaaggaac
     3541 gggcatcaag aaaatcacgg gcggcgaccc cgtcgaaatt aacccgaaat atgagaagcg
     3601 attcacgacg gtaataaggg cggtggtact ggcgaccaac aacaacccga tgatattcac
     3661 cgagcgggcc ggaggtgtgt cacgccgtcg ggtgattttc cggttcgaca acattgtaag
     3721 ggaggacgaa aaagacaagg aattaccgga aaagatagcg gcagaaatcc ccgtaattat
     3781 ccgccgcttg ctggctaatt ttgccgaccc tgaaaaggca cgggctttac tcctggaaca
     3841 gcgcgatggt gatgaagcac tggcgataaa gcaacagaca gatccggtta ttgagttttg
     3901 ccagttcctg aattttctgg aggaagcacg cggcctgatg atgggtggcg gtggcgattc
     3961 cgtgaagtac acgaccagaa acagccttta ccgcgtctat ctggcgttta tggcatacgc
     4021 aggaagaagc aagccgctaa acgtggctga gttcagcaag gccatgaagc cagcggcaaa
     4081 agtttacgga catgaatata ttacgcggag agttaaggga gtaacgcaga ccaacgcaat
     4141 tacaactgat gattgcgacg cgtttttata attttagaca aaagccctct acctcttcta
     4201 cctaaagaaa ataaatatac attattcaat atgataagtg ggtagagggg caggtagaag
     4261 gcaaataaaa gccctctaca ccctccacca tgattaaggt cgtttgtgag gggcgggtag
     4321 aggatgggta gagggccacg aaagccctct acccatctga aagccgcgcc attactgacc
     4381 tgaaagatga ttgggtagag aggtagagga ggtgcaccac aacctaaaac tttttaaaca
     4441 cgagggtaaa aataaaaatg cacacatcag gaaaattgaa caatcagaag aaaccacata
     4501 accgcgccat tgaccttaca gagcactggc tgagggtggc gataaaaatc atcgaccgcc
     4561 acacacggga aggatacgcg aaggagaatc ccgaactgat aagcgcattc atgacaacgg
     4621 cagctgcaaa ctttgccacg ctgacagaac gggagattgc cgaagcggaa caggtaacaa
     4681 ccatcaacgt taaaagcgct ggagaataac cgaagtagtg aacacatagc cggagcaatc
     4741 cggctttttt tcgatctcgc atatcttaaa aaatggttga ttgattataa tctatcgaaa
     4801 atactgatta ttagatgata agcatcagga ggaaacaaag tggaagagat aaagcaaagc
     4861 gctaccgtga tgattgatca cgagatccta aacgagctta aaaaacttca attcgatgtt
     4921 attcgccgaa tgaatgaagc tggtatatac ggcagagccg ccacgccaac aataggtcat
     4981 cttgcacgtg tgctgttacg cgaaagactg ggcattgcaa caggtgaggc ataacaaatg
     5041 gctatcacag aacgcgaggc aagcatcatt aaggccattg gcgaggaaac aagagacacc
     5101 atagcgccat tgatggaaaa gatcgcagag cttgagacac gaataaacaa gctgagtaat
     5161 gaagttgaag gcctgatctc gttactcggt gagcaatgac atggggggga gtgttccggc
     5221 cggaaagtcg atcaccctgg acaccgcccc ccgtctcatt cgtagaaaat tttgcttttc
     5281 aaaaaagtta cacgaaaagt tacaccccct caaaacgagg tttttgcatg gcattaacga
     5341 ccaaaaaaaa gcggtttgct gatgcgttat caaggggcgc aaatcccacg gaagccgcta
     5401 tacatgcggg atacagcgaa aagaccgcaa gggttaaagg ctggcagcta tccaacgatc
     5461 ctgaggtgaa aaagtatctt aaggctgtaa cttctgatgt aacttttcag gttacaaaac
     5521 cgaaaaaagt tacaaagaaa gttaccgaaa aagttacacc ggaacgggca tcaattaaca
     5581 ccattgagat gatggataac ggcctacccg accccatcaa agcaatgggc aggatactga
     5641 ttgacaacat ggacacagat ccaaaactgg ctttagatgc cgcgtttaag ctggcccagt
     5701 tcacccaccg caagatcggc agcattggga aaaaagaggt taaaaaactc aatgccgcta
     5761 gtgtctactc aaatcggttc cccatcccta agccaccaac agacttaaaa cactgaggta
     5821 gcgaactatg agcgaataca tcacagaccc tattgatgta ctcacggaaa tcatgaacga
     5881 caagcagcag tcgaccgaaa tacggttaca ggccgccgct gttctgatgc catattttca
     5941 cgaaacacta aacccgtgct tccaggagga ggaagaagat gatctgtaaa aacatgaaag
     6001 gcgtgtcagc agttcgcgcc gccagcgtgc tggcatcgtg taaatttagc catgctgacg
     6061 ctatagagat aacgaaaaga aatgatccgg cagtatcgaa atttcttgaa aaatccatca
     6121 tcaacagaat gactacggat aacatgccgt cgctggtggc agagccgata gtaagagagc
     6181 ttggcgagta tattttttcg cagtctgtac caggcaagct aatcagcagc gcgcttaatc
     6241 ttccgtttgg cgcaaaattg cattcaatat caggcaaggg tagtaaatgg cttaaagaag
     6301 ccgaagaaat ccctgtcaaa gagggagaag taaacgaaag tgcgctaaaa ttgtgcaaac
     6361 ttggcgctat tgtgatgctg aataacgagc taatcaaatt tagcacccct ggtacaaatt
     6421 cagcgatcag agacttgatc acatcggaat cggtgaaaga aatagataaa aaatttcttt
     6481 ccagtgatca ggaggtggct ggcgtatccc ctgagggcgt gcttaatggt gctgatgagg
     6541 ccgaaaactt catagagatt ttcaaaaagc acatagcgaa cggtaacacg ctttccacgt
     6601 cgtcgctaat attgcccgta gaaaacgcct tacagcttac tgatgctgag ttctggcaga
     6661 tagagctttt gagaataccg gtaattgtga gcgaacaggc ggatcgcatg atgctgatag
     6721 acgctcaaaa gctgattatt aacgttgaag ccacggttat aaccccaact gatgaggcgg
     6781 taataaaaac cgaatcaggg gcggttagcc ttttccagaa tgacgctaca gcgttcaggg
     6841 caatcaccta ttgcggatgg caaaaactcg ataaggccgt caccgttctg gcgaaaagct
     6901 gatttttcca gcatctatcc cgatatgggg atcaccatat cggggaagca cctgaatcat
     6961 cattaccgtg atcaagggta tgtaaagccc accagcctga ttaacgttta accggaaaaa
     7021 aagccaggta tccaatctcg atatggggat caccatatcg ggatacggcg gtaactggtt
     7081 gatattttcg aaattcgcca gcggcgaaca tcggggaaac cagcctaacc cgttgattct
     7141 tcccaatttc cacagactgc ggaaattggc gcggtaactt actgagatta aagcaaagcg
     7201 caaaattgcg ttggctggtg ggcctgatgc cgataactgg ttggaattgt tccatagtgc
     7261 aaaattgcac tatggcctaa cccattgatt atttcgaaaa cccccattgg gggatgttgg
     7321 gaaagtaaga ctaacccatt gattcttcca caatcctcaa tttgaggaga tcggaaacat
     7381 cacccttcaa attctgaatg gtcataatga ccacgcagac catcaaggca accacaacga
     7441 acatcgttac ggttggagct tttaccccac tggggaaaag gtacgcacct gaatcatcat
     7501 taccgtgatc aagggtatgt aaaggccacc agcctgatta acatttaacc ggaaaaaaag
     7561 ccaggtaggt ggggataccc atatcgggga acatccacca gcttcataac ggatggccgg
     7621 aaaatgataa cggttaaagc gtgttgatct ttgttcgtgg ttgttcgata ttgttcgtga
     7681 aacggtgtag ttaatggtgt agttaatttc gctgtttttg acggagttgt tatttgtaac
     7741 atagtgaaaa ataaagacta atcaatgaaa tgttataaat gatagttatc tatcatgtgg
     7801 agtagattgg tcagacaaat aagctcttgt cagcggcagg gcgttctgcc gataaccgta
     7861 accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg
     7921 cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca
     7981 atgatgatta cactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg
     8041 atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgtg
     8101 gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg
     8161 agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg
     8221 gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag
     8281 cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta
     8341 acccatctgc ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg ctccgtcagg
     8401 aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg
     8461 acacacagcc gacgcttagt tttcagcgtg tactgcaacg tgcggtcttc catgtccagt
     8521 cctccggtcg caatgaggtt accggtgcaa acgttctggt cgctatcttt agcgaacagg
     8581 agtcgcaggc ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat gtggtgaact
     8641 ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc
     8701 caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga
     8761 atcagcttgc gcgcgtgggc ggaatcgacc cactgattgg tcgtgagaag gagctggagc
     8821 gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctagtg ggggaatctg
     8881 gtgtcggtaa aaccgcgatt gcagaaggtc ttgcctggcg aattgttcag ggcgatgtgc
     8941 cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca
     9001 caaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg
     9061 acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg ggtgcagcgt
     9121 ctggtggtca ggtcgatgcg gctaacctaa tcaaaccgtt gctctccagc ggtaaaattc
     9181 gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc
     9241 tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc gatcgaagaa actgttcaaa
     9301 tcatcaatgg cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag
     9361 cggtgcgtgc ggcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata
     9421 aagcgattga tgttatcgac gaagcgggcg ctcgcgcacg tctgatgccg gtaagcaaac
     9481 gcaagaaaac cgttaatgtg gcggatattg agtccgtggt ggcccgtatt gcacgcattc
     9541 cagagaagag tgtttctcag agtgatcgtg ataccctgaa aaaccttggc gatcgcctga
     9601 aaatgctggt cttcggtcag gataaagcca ttgaggcgct gactgaagcc attaagatgg
     9661 cgcgtgcagg tttaggtcac gaacataaac cggtcggttc gttcctgttt gccggcccta
     9721 ccggggtcgg gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagcttc
     9781 tgcgctttga tatgtccgag tatatggaac gccataccgt cagccgtctt attggtgcgc
     9841 ctccgggata cgttggtttt gatcagggcg gtttgctgac tgatgcggtc atcaagcatc
     9901 cacatgcggt gctgctgctg gacgaaatcg agaaagcgca cccggacgtg ttcaatattc
     9961 tgttgcaggt gatggacaac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc
    10021 gtaacgtggt gctggtgatg accaccaacg ccggggtacg ggaaactgag cgcaaatcca
    10081 ttggtcttat ccaccaggat aacagcaccg atgcgatgga agagatcaag aagatcttta
    10141 caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt cgatcatctg tcaactgacg
    10201 tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagctg gatcagaaag
    10261 gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgataaa ggttacgacc
    10321 gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg
    10381 ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc gcgctggata
    10441 aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag
    10501 cgcattaatc tgattgtcag gtaggttggt gaagtccgta atctcgaaag aggttacgga
    10561 ctttttattt atggggtgga acggtgaaaa ccctattttt ggaggtgaag gtaatttgtt
    10621 gataattagt gctgctggaa aaggtaagga taaaaaaggg tgacagcagg aaaatgagat
    10681 ggttttgctt tattaacaac gggctaaacg tgtagtattt gagttcactg ccgtacaggc
    10741 agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg ccgtacaggc
    10801 agataaaatg cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa atatggcggt
    10861 gaggggggga ttgactcgct tcgctcgccc tgcgggcagc ccactcactg cgttcgtggt
    10921 ctgtccaact ggctgcgcca gttgtcgaac cccggtcggg gcttctcacc ccccctggag
    10981 tgcattatgc gaaaaaaaag ctcgcacttt cgtacgagct cttctttaaa tatggcggtg
    11041 agggggggat tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc
    11101 agccactcgg acacctcacc aaattgtttt gcagccaaac ctcatgggtg gcaacggggc
    11161 gctactatag ggatttggag taaaacggtc aagaagaatt ttaatgataa ttattgtttg
    11221 ctcatactgt aaacaagttg tgcagtatat ctacatcgag acaagttatg gacttatact
    11281 tccaaagtac ttcatacata tcacaaaata aaaaggccgg ttaaaccgac cttttactcg
    11341 ttctttctct tcgcccatca ggcggtaaaa caatcagcga ctacggaaga caatgcggcc
    11401 tttgctcagg tcgtacgggg tcagttcaac agtcactttg tcgcccgtca ggatgcggat
    11461 gtagtttttg cgcattttac cggagatgtg tgcagtaacc acgtgaccgt tttctaactc
    11521 tacgcggaac atggtattag gcaacgtttc aagaacggta ccttgcattt caatattgtc
    11581 ttctttggcc atctaatcct ctggggtatc actaccgtaa tttgaaccgg caagataatg
    11641 ccgaagttct gtaaataagt aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac
    11701 attactccga aaacacacgg ctaagccgca ccaaaagcgc aacgtataag ggagcggtga
    11761 gataaacgat gggcgttacc tgacgcgaaa aattccttat cggcagcggg gtaatgagcg
    11821 taaccaactc tgcgaccgca attataacac tctggggaga aatgtgccga aaacattcat
    11881 tcttgtggtg aaaacaagca tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc
    11941 atttgattga gataattaag gtaatcccgg cggggaattt cgcaggcacc aagcgatgct
    12001 gtgtgatcgt taaggacctg gcagtcgata agcttaccgc catgaccgat aaattcctca
    12061 cagaatacca gaagcgccgt tttagacgca ttttccatcc ggctgaacat ggactcgcca
    12121 caaaatagcg ttccctgggc cacgccgtac ataccgccga caagcccatc ttcacgccag
    12181 acttcaatgg agtgggcatg accgagttcg tgaaggcgat ggtaggcttc gaccacgcca
    12241 cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa
    12301 gcgtaattca tcgtgacacg atagggcgag cgtttatgaa atcgcttcat actacggctg
    12361 atatgcagtg attctggcca tagcaccgcg cggggatcgg gcgaccacca gaggatgggg
    12421 tcgcctggag aaaaccacgg aaaaatacca cgctggtaag ccattaacag gcgcgcaggg
    12481 ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa
    12541 gggaaggcta ttgaatggcg agaaagctga accaggcgca tgaccgcaaa actccacgca
    12601 agtcggatcg ttcaataata gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc
    12661 tggcaagcag ttctgcgtga gtaccttgct caataatttg cccgttgtcc atcactatta
    12721 tttgttggaa acgagagagt ccgcgaagtc gatgggtgac cattaacacc gttttctcac
    12781 gcatcatttc tgcaagcaat tcaaggatct ggctttcggt tgtggcatct aagccttcgg
    12841 taggttcatc cagcaacacc agtggcgcat catgtaacag cgcacgggcg atagccagac
    12901 ggcgcagttc accaccggag agctggcgtc cgccttcacc taaccaactg ttgagacctg
    12961 catcctcgag cagcttctcc agaccaacgc gacgcaagat ctccgacaga gcctcatcac
    13021 tactgccagg cgaggcgagt aacagattat cacgcagcgt ggcgctaaac agatgcactc
    13081 gctgaggaac aacgctgatg gtctgtcgta gagccgcttc attcaggctg gctatggggc
    13141 tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc gcgggtcagc agttgtaaca
    13201 gtgttgattt gccgcatccg gttcgcccga gaatcgctat atgttccccg gcgtttacct
    13261 gaagagaaat ccctttaagt gcctgttggg attgctccgg ataagtgaac tgaacatccc
    13321 gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt atcagggaag gtgacctccg
    13381 gtttttgatc cgttaagtca gagatacgta cggcagaggc aatgacttgc cccagatgct
    13441 gaaatgcacc cgttactggt gccagtgctt caaacgcggc taacgcacaa aagacaaaca
    13501 gggcaattaa cgcgccgggt tgagcattgc cgccaacgcc gccagacgcc atccacagca
    13561 tcaggatcac cgctaacgcg ccaatgagca gcattatcgc ttgcgacaat gcggtcagtt
    13621 cagattgacg gcgttgcgct tccagccatt gaatttctgt attctctagt tgcgtgcgat
    13681 aacgatcgct ggcaccaaaa atggtcagct cagcttgccc ttgcagccag gccgtcagtt
    13741 gttggcgata ctgtccgcga agatgagtca gattttgccc ggtgcttttt cccgcacgat
    13801 aaaacagcgg tggcatcagg aaaagcgtca gtaacataat gctgcccagc gtaaaggcga
    13861 gggtgaaatc aaggaaactt aacccgattg tcaccaccat aatcaccaca aaagcgccca
    13921 ccagcggcga gataacgcgc aggtaaagat gatcgagcgt atcaacatcc gccaccacgc
    13981 ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc aggggagagg ggcaacaatt
    14041 tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa agtcgcgtcg tgacttacca
    14101 gacgttcaaa ataacgcccg gcagtacggg tgattgcggc accacgcacg cccgcagcgg
    14161 gtagcatata gttgaagctg tacagtccgg caacccccgc aaccgctgag gccgagagga
    14221 accagccgga aagtgtcaac agaccgatac tggcgagcag cgtcacaatt gccagcacaa
    14281 taccaagact taacatccat ttatgacgtt tatacagtgc cagatagggt agcaaagcgc
    14341 gcatttaaat ctcctcctga cgatgggcca gtaatgtggc gaatgggcca ccagccacac
    14401 ttaattccgc gtaacgtcct tgctcaataa tccggccatc ctgcataacc caaatgacat
    14461 cccagtcagc aagatcttct aactggtggg tgaccattaa cgttgtctgg cgcagagagg
    14521 cggcattcag cgcctccatt acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt
    14581 catccaacag taatagcgaa cagggattta gtaacgcacg ggccaccgcc acgcgctgcg
    14641 cctgccccac ggaaaggcgg gcagcctggt cgccaacagg tgtatcaacg ccttgtggca
    14701 ggagcggtag aaactcgctg acccaggcgt tatccagcgc tgcttgtaat tcttgttcgc
    14761 tggcatcagg tcgcgccagt agtacgttat cccgcaatgt tgctgccggt aattgtgggt
    14821 tttgcccaac ccaggagaga tgtttacgcc atgattctgg tgataaatca cgtaattcta
    14881 tcccgttgat tcgtaatgat ccctgatatg agagaaaacc agaaagcgcg ttcagcagtg
    14941 agcttttacc tgaaccgctg cgaccaacca acactgcacg ttggcctgct ggcaaagtaa
    15001 agttcagcgg tccggccagc gttttacctt ccggcgacgt gataaacaga tcctcggcct
    15061 caatggtcag cggatcggtc aatgctaatt ccgcctcacc gcgctgcgga tgggcgagcg
    15121 gggtttccat aaacgttttc agactgtcag ctgcaccaac agcctgggct ttagcatgat
    15181 aaaacgtacc gagatcgcgt aatggctgga aaaactctgg cgcaaggatc agggccagaa
    15241 aacctgcagc cagcgtcacg ccagtatcgt agtgaccaaa atccagctcg ccgagatagg
    15301 aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc
    15361 cggaggataa aaacgccagt cgtagcactt ccattgtccg ttggcggaaa tcttccgaag
    15421 cagaacgaat actttcaatt tcagcttcac cacgaccaaa aatacgcaat gtttccatgc
    15481 cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag agcgagaaag ttacgtcggt
    15541 tagcatcggc agcccccatt ccaaccagcg ccataaacaa cggaattagc ggtgctgtgc
    15601 ccagcagaat gagcgccgca gcccagttag aggggaagat cgccaccaca atcagcaacg
    15661 gcaccgacac tgccagcgcc atttgcggca gatagcgtgc atagtaatca tgcatatcgt
    15721 caatttgctc gagtaccagc gtcgcccagc tccccgcagg tttaccctga atccacgctg
    15781 gccctgcttg ttgcagacgg tcgagaacct gacggcggat ggcaaagcgg atatgctgcc
    15841 cggcgtgata acccacccgt tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca
    15901 gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg aatattctcc ataatcatat
    15961 gttgcagaat tcgcgccatg aaccaggcct gggcaatgat caatatgccg cttacaaagc
    16021 ccagcagacg agaaatattc agccaacgtt gggagatgac gctttgctgt tttaaccagc
    16081 gggttaactc tttttgacga gatttattca ttgcacgctt agcaggtgag ttatcagaat
    16141 tatttgcaga gcaatgttac aacggggaaa aaataaaggc gacccatagt cgcaaggtgt
    16201 cgccttcttt acttttgtta ctgatttgta aaattatttt gcgtcagcta aaccatcgag
    16261 gtagcgttcc gcatcaagtg ctgccatgca gcctgtaccg gccgaagtaa tggcctggcg
    16321 ataaatgtga tccatcacgt cgcctgcggc aaagacgcca ggaatgctgg tctgggtggc
    16381 attaccatga atacccgact gtactttgat gtagccgttt tccagttcca gctgcccttc
    16441 gaaaatggca gtattcgggc tgtgaccgat agcaacaaac agaccggcaa cgtcgagtga
    16501 ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg cctgtgacac ccatttgatc
    16561 gccgctcact tcttccagcg tacggttggt gtgcagaatg atgttgccgt tctccacttt
    16621 atccatcaga cgcttaataa ggattttttc cgcgcggaaa ccgtcacggc ggtgaatcag
    16681 atgcacttcc gaagcgatgt tagacagata cagcgcctct tcaaccgcgg tattgccgcc
    16741 gccgatgacc gcaactttct ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga
    16801 aaccccacgg cctttaaagg cttcttcaga gggcaggccg agatagcgtg cagaagctcc
    16861 ggtggcaata atcagcgcgt cgcaagtgta ttcaccgcta tcgccagtaa gacggaacgg
    16921 acgattctgc aaatcaactt tgttgatgtg atcaaagatg atttcagttt caaatttggt
    16981 ggcatgttcg tgcatgcgct ccattaataa cggaccggtc agatcgtttg gatcgccagg
    17041 ccagttttcc acttccgtgg tggtggtcag ttggccgcct ttttccatgc cggtaatcag
    17101 cacaggttgc aggttggcgc gcgccgcgta gacagcagcg gtgtatcccg ccgggcctga
    17161 acccaggata agcagtttac tgtgtttggt cgtgcccatg agatccccat agttgttggc
    17221 agacaatggg caggattgta gggaatttac cgacgtaaaa aaagagtatg acgattttgt
    17281 taacaatttg tgcaatcggc agcatcgata agcaggtcaa attctcccgt cattatcacc
    17341 tctgctactt aaatttcccg ctttataagc cgattaaatg atgaataaac gcccctggta
    17401 atgaatatct ggcatgttgt actaaaaatc gatgttttgc tttgacaatc ccctggtgtt
    17461 ttgcgaaaac attcgaggaa gaaaaaaaca gtattcttat atgcgcataa ccatgcatgt
    17521 aaataccatg tttaccgtgc tagtgaaatc tacgtatggc gtggacagac gccattcgtg
    17581 atgtcgatag ctgccacaag gcaacggtct tctcaccgta gacccaggca ttgcgcgccg
    17641 tgaatcttta tgatttcggt ctatcgtgac gggtagcgac tctgaacagt gatgtttcag
    17701 ggtcagacag gagtagggaa ggaatacaga gagacaataa taatggtaga tagcaagaag
    17761 cgccctggca aagatctcga ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat
    17821 gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac tttccccaac gccctgcctt
    17881 gagcgtgtgc gtcggctgga aagacaaggg tttattcagg gctatacggc gctgcttaac
    17941 ccccattatc tggatgcatc acttctggta ttcgttgaga ttactctgaa tcgtggcgca
    18001 ccggatgtgt ttgaacaatt caataccgct gtacaaaaac ttgaagaaat tcaggagtgt
    18061 catttagtat ccggtgattt cgactacctg ttgaaaacac gcgtgccgga tatgtcagct
    18121 taccgtaagt tgctggggga aaccctgctg cgtctgcctg gcgtcaatga cacacggaca
    18181 tacgttgtta tggaagaagt caagcagagt aatcgtctgg ttattaagac gcgctaacac
    18241 ggaacaggtg caaaatcggc gtattttgat tacactcctg ttaatccata cagcaacagt
    18301 actggggtaa cctggtactg ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg
    18361 gagagccttt cttgagccag gaatacactg aagacaaaga agtcacattg acaaagttaa
    18421 gtagcggccg ccgccttctg gaggcgttgc tgatccttat tgtcctgttt gccgtctggt
    18481 tgatggctgc cttactaagc tttaaccctt cggaccccag ctggtcgcaa acggcctggc
    18541 atgaacctat ccataattta ggtgggatgc ccggtgcgtg gttggcagat acgctgttct
    18601 ttatttttgg cgtgatggct tacaccattc ccgtcattat tgtcggcggt tgttggtttg
    18661 cttggcgtca tcagtccagc gacgaataca ttgattattt tgccgtttcg ctacgcatca
    18721 ttggcgtttt ggcgctcatc cttacctcct gtggtctggc ggcaatcaac gctgacgata
    18781 tctggtattt tgcctccggt ggcgtcattg gcagcttact aagcactacg ctacaaccac
    18841 tgctacacag tagcggggga actattgcgc tgctctgcgt ttgggcagcg ggcctgacgc
    18901 tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact cggcggctgg attttaaaca
    18961 ttctcacctt cgccagtaat cgtacccgtc gcgatgatac ctgggtcgat gaagatgaat
    19021 atgaagatga cgaagagtat gaagatgaaa accacggcaa acagcatgaa tcacgccgtg
    19081 cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc
    19141 cgatggggcg gcaaacagat gctgcgctgt tctccggtaa gcggatggat gatgaagaag
    19201 atattaccta cactgcacgc ggtgtggctg ccgaccctga cgacgtccta ttttcgggca
    19261 atcgtgcaac gcagccagaa tatgacgaat acgatccatt attaaatggt gcgccaacta
    19321 ccgaacctgt cgctgtagca gctgctgcta ccacggcgac acaaagctgg gctgcgccgg
    19381 ttgaacctgt gactcagacg ccgcctgttg cctctgttga tgttccacct tcgcaaccta
    19441 cagtagcctg gcagcctgta ccgggtccac aaacgggaga gccggttatt gctcctgcac
    19501 cggaaggtta cccacagcag tcacaatatg cgcagcctgc agtgcaatat aatgagccgc
    19561 tgcaacaacc agtacagccg cagcagccgt attatgcacc tgcagctgaa caacctgcgc
    19621 aacaacctta ctacgctcct gcggcggaac aacctgttca gcaaccgtat tacgtaactg
    19681 cgccggaaca gcctgcgcaa cagccgtatt atgcccctgc gccagaacaa ccggtggcag
    19741 gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga
    19801 ctgagcaaac ttatcagcag ccagccgctc aggagccgtt gtaccaacag ccgcaacctg
    19861 ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc
    19921 cgccgcttta ctactttgaa gaagtggaag agaagcgagc ccgtgaacgt gaacaacttg
    19981 cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgc
    20041 tgaaagcacc ttctgttgca gcagtacctc cagtagaagc cgctgccgct gtttctccgc
    20101 tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc
    20161 cagtcttcag tctggcaaat agcggtggac cgcgtccgca ggtcaaagag gggattggcc
    20221 cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg tcgtgaattg gcgtcttacg
    20281 gtattaagct gccctcacag cgtgcggcgg aagaaaaagc ccgtgaagcc cagcgcaatc
    20341 agtacgattc tggcgatcag tacaacgatg atgaaatcga tgcgatgcag caggatgaac
    20401 tggcacgtca gttcgcccag acacagcagc aacgctatgg cgaacagtat caacatgatg
    20461 tgcccgtaaa cgcagaagat gcagatgctg cggcagaggc tgaactggct cgtcagtttg
    20521 cgcaaactca acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc
    20581 tggatgattt tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgt
    20641 tgtttacgcc aattgttgaa cctgtacagc agccgcaaca accggttgca ccgcagcagc
    20701 agtatcagca gccacaacag ccggttgcgc cacagcagca atatcagcag ccgcaacagc
    20761 cggttgcgcc gcagccgcag tatcagcagc cacaacagca ggttgcgccg cagccacaat
    20821 atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg caacaaccag
    20881 ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcaccgcag ccgcagtatc
    20941 agcagccaca acagccagtt gcgccacagc cgcaggatac cctgcttcat ccgctgttga
    21001 tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct tctctggatt
    21061 tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg
    21121 ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc gtcaattact
    21181 ctccggggcc ggttatcact cgctttgaac ttaacctggc accgggcgta aaagcggcgc
    21241 gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg
    21301 ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa aaacgacaaa
    21361 ccgtttatct gcgcgaagta ttggataacg ccaaattccg cgataatcca tcgccattaa
    21421 ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc
    21481 cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg aacgcgatga
    21541 tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga
    21601 aaatgctgga gctttcggtg tatgaaggca ttccgcacct gttaacggaa gtcgttactg
    21661 atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag cgtcggtata
    21721 aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag
    21781 ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac agtatggatg
    21841 ctcagcatcc ggtgctgaaa aaagagccat acattgtggt gctggtggat gaatttgccg
    21901 acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg gcgcaaaaag
    21961 cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt gatgttatta
    22021 ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg
    22081 actcacgcac cattcttgat caggctggcg cggaatcact gctgggtatg ggggatatgc
    22141 tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt gttcgcgatc
    22201 aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg
    22261 gcatcacctc cgacagcgaa agcgaaggtg gtgcgggtgg tttcgatggc gctgaagaac
    22321 tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcgtcaattt
    22381 ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga
    22441 tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc
    22501 caccgccgtt cgactaatta atgcattgcc ggataaggcg cggtagcgtc gcatccggca
    22561 ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat
    22621 agagagtaga gggaactccc ggtcgggagt gacgtaattt gaggaataat gatgaaaaaa
    22681 attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc
    22741 gcaagcgatc tgaaaagtcg cctggataaa gtcagcagct tccacgccag ctttacacaa
    22801 aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa
    22861 cgtccaaact tattcaactg gcatatgact caacctgatg aaagcattct ggtttctgac
    22921 ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac ctggctgaaa
    22981 gatgccaccg gtaatacacc gtttatgctg attgcccgca accagtccag cgactggcag
    23041 cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc cagcaatggc
    23101 aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg
    23161 gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa tggggctgtg
    23221 gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag
    23281 tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct
    23341 ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggtcagcaac atttgctggc
    23401 tgcggggaag ccgttgccgc gcgctatcga agccgggcat ttacattcta tgatcctctg
    23461 ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc
    23521 tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg aggcgatcga
    23581 gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca
    23641 ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac
    23701 ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg
    23761 tgcccgtgtt tatctgttga aatccctgag tacagaggat attgagcaag tactaactca
    23821 ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc cagatgaaac
    23881 acgacgtgcc attgcagaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga
    23941 aatgatggcg gatatggccg aagtcgatga tagcggtaag cgggtcctga agcctgaatt
    24001 actgaccgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg atcgctttta
    24061 cgatctgatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctgta
    24121 ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc gtcgctgtct
    24181 ggcgattgcg tctgaagacg tcggtaatgc cgatccacgg gcgatgcagg tggcaattgc
    24241 ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc
    24301 gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc
    24361 gctggccgat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc gtaatgcgcc
    24421 gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc atgatgaagc
    24481 aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta
    24541 tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct ggctggctga
    24601 acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt
    24661 tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag
    24721 gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact
    24781 ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag agcgtcgtaa
    24841 agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactctcgat cgaaatccat
    24901 cggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact
    24961 gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga
    25021 tatcgcgctg acaatcccta acctgcctgc agatgaagtg ccggttggta aagatgaaaa
    25081 tgacaatgtt gaagtcagcc gctggggtac cccgcgtgaa tttgacttcg aagttcgtga
    25141 ccacgtgacg ctgggtgaaa tgcactccgg cctcgacttt gcggcagcgg tgaagctgac
    25201 aggttcgcgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg cactgtcgca
    25261 gtttatgctg gatctgcata ccgaacagca tggctatagt gagaactatg ttccgtacct
    25321 ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt
    25381 ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc
    25441 agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg atctgccaat
    25501 taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg gtcgtgacac
    25561 ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc
    25621 ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag tcctgcagtt
    25681 gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct ttggcgcttg
    25741 caaaacttac gacctggaag tatggatccc ggcacaggac acctaccgtg agatctcttc
    25801 ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc gcagcaagtc
    25861 ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg ttggtcgtac
    25921 gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt
    25981 tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct
    26041 aaaaagcgcc tacgggcgct ttttttgtct ccctttgata ccgaacaata attactcctc
    26101 acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaatcat
    26161 tcgatgtata caatcctata tagcgatctg ctatagaaat aattacacaa tacggtttgt
    26221 tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa atccctgatg
    26281 cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc
    26341 tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata
    26401 gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg
    26461 gtagtcgctg cccgctacgt atgcacgtag tggacggtga aatcaaatat gtcgaaacgg
    26521 acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc ctgcgtgggc
    26581 gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg
    26641 gggcgcgcgg tgaaggcaaa tttgagcgca ttagctggga agaggcctac gacatcatcg
    26701 cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat ctgaactatg
    26761 gcaccggtac gctgggtggc accatgaccc gctcctggcc gccgggaaat accctggtcg
    26821 cgcggctgat gaactgctgc ggcggttatc tgaaccatta cggtgactac tcttccgcgc
    26881 aaattgcgga aggtctgaac tatacctacg gcggctgggc agatggcaac agcccgtcgg
    26941 atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga
    27001 gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat gcccgcatga
    27061 tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag tggatcccta
    27121 ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg atcactgaaa
    27181 acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag aaaaccctgc
    27241 cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa ggaccagatg
    27301 gcgtggctaa aacaccggaa tgggcctcgc aaatcaccgg tgttccggca gacaaaatca
    27361 tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag ggatggggcc
    27421 cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga
    27481 ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggctca tacagtttac
    27541 cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg atgtttatgt
    27601 ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct acgtgatggt gtgcgcggga
    27661 aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta
    27721 accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag aagtgcgagc
    27781 tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac atcctgctgc
    27841 ctgactgcac cgcttccgaa cagatggact ttgcactgga tgcatcctgc gggaatatgt
    27901 cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag accatctatg
    27961 aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact gaaggccgta
    28021 cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc
    28081 caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca caagggcatc
    28141 acgttgctta taaagccttc cgtgaagatc cgcaggcaaa tccactgacc acgccatcgg
    28201 gtaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg gaattgccag
    28261 aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt tatcaggatc
    28321 cgctgaacaa acagtatccg ctgcagctta caggtttcca ctataaatct cgcgttcact
    28381 caacttacgg caacgttgat gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc
    28441 cgcttgatgc ccaaaaacgc ggtatccaca acggcgataa agtcaggatc tttaacgatc
    28501 gtggtgaggt tcatattgag gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac
    28561 tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt ggttgtatca
    28621 acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc
    28681 ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt
    28741 gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat
    28801 ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggtggcga ctggcaggaa
    28861 gatgacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac
    28921 tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgtgaagat
    28981 ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca tatggcttgc
    29041 ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt
    29101 tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc gctgcgggcg
    29161 ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg
    29221 ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa tgccaatagc
    29281 cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg agatgggaag
    29341 tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tggcgggtgg
    29401 ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaaa cccagcagcg
    29461 tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta tcgcctctat
    29521 gctccatctt ggttcaccga tgcgcgcctt taactcgctc aaccgggtag gggcttcagc
    29581 actcagtaac gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt
    29641 gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctgtggctgg tagtgacgat
    29701 ggttcttggc gtagtctttg tctggatgat ggtgcgcgtc tataacagca ttgataccgt
    29761 tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt tcatgggcgg
    29821 cccgttactg ggttacctgt tgctgagtct ggctggcgtc gatggctggg cgatgcgtct
    29881 gctgccagcc atttctgtac ttgcactggt agtgagtggc gtgatgtcag tgatgcaggg
    29941 cgcagagctg gtgactattc atagttctgt gcagcaggcc gcagcgctgg tgccggacta
    30001 tggtgcgctg atgtcctggc ggatcgtgct tttggctgtt gccctgtgct tgtggattgc
    30061 accacagcta aaaggttatc agcctgcggt tccgctacta tctgtctcat tcattctgct
    30121 tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga ccgtggggat
    30181 ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct tttatttctg
    30241 cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatgt ggttagagaa
    30301 caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgtagctcgc aagccacgcc
    30361 gaaccatgtc atcaattgcg caccagcttg cgacatgcga tcccacgccg aatgccgggt
    30421 aatttcatta aaggtccctg atgcgtcagt cacaacaaac acatcaaacc cttcttcaat
    30481 ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa taattaactg
    30541 ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg cgttaatatt
    30601 gccagggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg gaactaatgg
    30661 gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga aatacttagc
    30721 caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat cccgtacaag
    30781 ggaaagtaaa ccagcctggt gatcaacaag caaaacggca gcatcatttt tatcaagacg
    30841 aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc tgaaatcggt
    30901 cagacgcgat gcattgctct gaaagcatag acgggaaata tgagtttgct gtgaccatga
    30961 aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa caatcagatg
    31021 aaattaatca tctcttcagc attagcgtta tgaatgagta atataaatcg ataataccgc
    31081 cgctgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg catgatgcgc
    31141 atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg cctgtcatgc
    31201 ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat acactcgtgc
    31261 cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc agctcatcct
    31321 attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa cgcattggct
    31381 ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcg ggccttggcc
    31441 tgatgattgg tttctggagc tggttggcgt ggcgttttgt cgcgggcgtc ggctgtgcca
    31501 tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt aaccgtgggc
    31561 gtttgcttgc tgcgtatatg atggtttatt acgtgggaac gtttttaggc cagttactgg
    31621 tcagcaaagt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt ttgacgttgg
    31681 cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa aaccatgatt
    31741 cgacgtcaat tacgtcaatg ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca
    31801 ttatctcagg aatcgttctg ggatctctat atggcctgat gccgttgtac ctcaatcaca
    31861 aaggggtgag caatgccagc attggtttct ggatggcggt actggtcagt gcgggtatcc
    31921 tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg gtgttgcgtg
    31981 ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct tagcctggcg gcgatggccc
    32041 cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg atggcctggg
    32101 cttgtgagaa agttgaacat catcaactgg tggcgatgaa ccaggcctta ctgttgagct
    32161 ataccgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag aatttctccg
    32221 ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg atgctgcttc
    32281 gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca agcagagtgt
    32341 gaatttactg tttcacactc tgcttttttg tttcttctat ctgacttgct tttttacaaa
    32401 ttttattcgt ttaaaaataa aatgtgcagc aggttataat tttgcatttc gctatttccg
    32461 cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctttaa ttttggcgaa
    32521 ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca acattgcgct
    32581 gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactatg ccaaccaggg
    32641 gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac cttatgcgct
    32701 aattgttgga cagttaggct cgaccttcaa agatgggaag ggcggggtca gtacctggat
    32761 taaacacacg atgggacccg gactggctta cctcgccgcg tggacctact gggtggtgca
    32821 tattccctat ctggcacaaa aaccccaggc aatactgatt gcgctcggtt gggcgatgaa
    32881 aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt taacgctggt
    32941 gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga aaatcgtcgg
    33001 ttctgtggca gggattgcga tgtttgttat gtcgctcctg tatgtggtga tggcggtaac
    33061 cgcgcctgcg attactgaag tgcatattgc gaccacaaac attacctggg aaacgttcat
    33121 tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg cggttggcgg
    33181 agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag aatttccaaa
    33241 agggatgcta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct cactggcgat
    33301 ggggatgatg tttgactcgc gtaatatccc ggatgactta atgactaacg gtcagtatta
    33361 cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg tgatttacgc
    33421 cattgcgaat accctgggac aagtagctgc gctggtattc tcgattgatg ccccgcttaa
    33481 agtgctatta ggcgatgctg acagcaaata tattccagcc agtttatgtc gtaccaacgc
    33541 ttctggtacg cccgttaatg gctattttct gaccctggtg ctggtggcga tcctgattat
    33601 gctgccgact ctcggcatcg gtgatatgaa caatctctat aaatggctgt tgaatcttaa
    33661 ctcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg cagtcgttcg
    33721 cctggcgcag aaatatacac cagagtatgt ctttattcgt aataagcctc tggcaatgac
    33781 cgtcgggatt tggtgttttg cctttactgc ctttgcctgt ttgacgggga tcttcccgaa
    33841 aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg caacgccgtt
    33901 tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga atagtaaata
    33961 attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca taatcgagaa
    34021 atgcgcgcaa gccgcaggac atatactggc ggtttgggta atacatttgg aagctgtgct
    34081 gtaagggcgt tagaaataga gctctgaatt atttatgcag cgcggaataa aaaagcggca
    34141 actcaataaa gttgccgctt tacggggaaa ttagaacatt accttatgac cgtactgctc
    34201 aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac catcgagttt
    34261 gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg ggagaagttc
    34321 gattttctca acgttaccca tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc
    34381 atcgtcagac cagcctggga caacaacgta gcggatccac accttcacat ttttattcgc
    34441 cagatatttg gcgaactcca gcgtgcggtg gttggaaact ccaaccagat tttggtggat
    34501 ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca gcagttcatc
    34561 aatcaccgga tcgtaacgac gaacaaaacc gttggtgtcc agacaggtat gaatgccttc
    34621 ttttttgcag gcgcggaacc agtcacgaac aaactcagct tgcaggattg cttcaccgcc
    34681 ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacttcctt
    34741 catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt cgcggttatg
    34801 acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacccgggcc
    34861 gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt
    34921 tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc
    34981 tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg tgctaaaaaa
    35041 ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc agtcaaatct
    35101 aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc tttagtcagc
    35161 gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata tttttccggg
    35221 ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag gtgctgacca
    35281 ccttcaatgg acgcttcgtg gtggaagtaa ccatccatca gaccagccag gttggtctta
    35341 cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta ggagatacca
    35401 tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac agcacctttc
    35461 tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg
    35521 tctggggtgt tacccgtttt cttaccatac acaacgttag aagtgatggt cagaacagac
    35581 tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct
    35641 accaggtcaa cagccaggtc atctacacgc ggatcgttgt taccaaactg cgggtattcg
    35701 ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt aactttcgca
    35761 tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc acacgccatg
    35821 gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg
    35881 tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca gtccatgaag
    35941 tgatccatgc gctccatcac ttcatcgtag ttcaggagat cgcctttgat cggttcagac
    36001 ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc gtacagcatg
    36061 gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac gatcatcggg
    36121 cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg
    36181 ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt cttgaagttc
    36241 agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg acccatggtg
    36301 tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc gtcgaggccc
    36361 ataccaccga tagattcggt tgcccagatc gggtcgccag agaacagttc atcgtactcc
    36421 ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac catttcctgc
    36481 gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac atccaggaag
    36541 gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc agccaggtag
    36601 ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga gatgtcgtag
    36661 ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc agcgatttct
    36721 tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc ctgcagagag
    36781 gtgaactgag cgtatttgtc tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg
    36841 tagtcaccga tgatacggcc acggccataa gcatctggca gaccggtcag aacaccggat
    36901 ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta
    36961 cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta cgctttgcag
    37021 gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag cggagcttca
    37081 gtctgcagac caacaacttt ttccaacgct ttgttgatgt agccagcgtc gtgagaggtg
    37141 atggtggaag caacagcggt gtcaaagtca actggcgcgt gagtgcggtt ttccagttta
    37201 acgccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc agccaggaag
    37261 gactcgtcac cctcgtacgg agtgtagttt ttctgaatga agtcacggac gtttacttca
    37321 ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc attaagctcg
    37381 gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt cgacaaccat
    37441 taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac caacaaacca
    37501 ccgccgataa tattaccgat cgtaaccgga atcaggttat cagtgatgaa gttcatcacg
    37561 gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc cggagatgcg
    37621 aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact gtgctcaaaa
    37681 ccgctggcaa caaacatcgc gaccggcagc accatgataa acgctttgtc catcaggctg
    37741 cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag gataccaagg
    37801 cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag gacgtttagt
    37861 ccccattgac catttgcggt catatactcg ccggaaagcc acattaaaag tacaaacagc
    37921 agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttagccaa ctgaccccag
    37981 gtgatgcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa gagatcggct
    38041 ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac cagttttgcc
    38101 atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga tatagaagac gaatgcgatt
    38161 gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg tttcgttgct
    38221 ttatagacac ccgcctcttc ggccactttg gccattgcag caggaagtaa aagatcaaaa
    38281 gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga tactaacaaa
    38341 gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg cccgtaactc
    38401 gcatggtttt tatgcaaata cggagtaaat attt
//
LOCUS       DAZUGK010000021        37547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZUGK010000021 DAZUGK010000000
VERSION     DAZUGK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.021"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..120
                     /locus_tag="ACHY5W_000957"
     CDS             <1..120
                     /locus_tag="ACHY5W_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4445894.1"
                     /translation="ADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(183..737)
                     /gene="ytfJ"
                     /locus_tag="ACHY5W_000958"
     CDS             complement(183..737)
                     /gene="ytfJ"
                     /locus_tag="ACHY5W_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS4445895.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1062..1268
                     /gene="ytfK"
                     /locus_tag="ACHY5W_000959"
     CDS             1062..1268
                     /gene="ytfK"
                     /locus_tag="ACHY5W_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFS4445896.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1330..2673)
                     /gene="paeA"
                     /locus_tag="ACHY5W_000960"
     CDS             complement(1330..2673)
                     /gene="paeA"
                     /locus_tag="ACHY5W_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HFS4445897.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDSKDKEESVA"
     gene            complement(2996..3634)
                     /gene="msrA"
                     /locus_tag="ACHY5W_000961"
     CDS             complement(2996..3634)
                     /gene="msrA"
                     /locus_tag="ACHY5W_000961"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4445898.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            3840..5573
                     /gene="tamA"
                     /locus_tag="ACHY5W_000962"
     CDS             3840..5573
                     /gene="tamA"
                     /locus_tag="ACHY5W_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFS4445899.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGVSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5570..9349
                     /gene="tamB"
                     /locus_tag="ACHY5W_000963"
     CDS             5570..9349
                     /gene="tamB"
                     /locus_tag="ACHY5W_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFS4445900.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVIGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINNLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEINSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVCVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9352..9693
                     /gene="ytfP"
                     /locus_tag="ACHY5W_000964"
     CDS             9352..9693
                     /gene="ytfP"
                     /locus_tag="ACHY5W_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFS4445901.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            9905..10156
                     /gene="chpS"
                     /locus_tag="ACHY5W_000965"
     CDS             9905..10156
                     /gene="chpS"
                     /locus_tag="ACHY5W_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFS4445902.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10150..10500
                     /gene="chpB"
                     /locus_tag="ACHY5W_000966"
     CDS             10150..10500
                     /gene="chpB"
                     /locus_tag="ACHY5W_000966"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFS4445903.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10580..11110)
                     /gene="ppa"
                     /locus_tag="ACHY5W_000967"
     CDS             complement(10580..11110)
                     /gene="ppa"
                     /locus_tag="ACHY5W_000967"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFS4445904.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11420..12376
                     /gene="ytfQ"
                     /locus_tag="ACHY5W_000968"
     CDS             11420..12376
                     /gene="ytfQ"
                     /locus_tag="ACHY5W_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFS4445905.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLMLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12516..14018
                     /gene="ytfR"
                     /locus_tag="ACHY5W_000969"
     CDS             12516..14018
                     /gene="ytfR"
                     /locus_tag="ACHY5W_000969"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFS4445906.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14032..15054
                     /gene="ytfT"
                     /locus_tag="ACHY5W_000970"
     CDS             14032..15054
                     /gene="ytfT"
                     /locus_tag="ACHY5W_000970"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFS4445907.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPHLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15041..16036
                     /gene="yjfF"
                     /locus_tag="ACHY5W_000971"
     CDS             15041..16036
                     /gene="yjfF"
                     /locus_tag="ACHY5W_000971"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFS4445908.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIDFTGVFLAKVIGDFGLSPLLAFPLVLGMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16069..17067)
                     /gene="fbp"
                     /locus_tag="ACHY5W_000972"
     CDS             complement(16069..17067)
                     /gene="fbp"
                     /locus_tag="ACHY5W_000972"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFS4445909.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            17243..18616
                     /gene="mpl"
                     /locus_tag="ACHY5W_000973"
     CDS             17243..18616
                     /gene="mpl"
                     /locus_tag="ACHY5W_000973"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFS4445910.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(18772..19323)
                     /gene="yjgA"
                     /locus_tag="ACHY5W_000974"
     CDS             complement(18772..19323)
                     /gene="yjgA"
                     /locus_tag="ACHY5W_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFS4445911.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19417..20769
                     /gene="pmbA"
                     /locus_tag="ACHY5W_000975"
     CDS             19417..20769
                     /gene="pmbA"
                     /locus_tag="ACHY5W_000975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFS4445912.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            20953..21339
                     /gene="cybC"
                     /locus_tag="ACHY5W_000976"
     CDS             20953..21339
                     /gene="cybC"
                     /locus_tag="ACHY5W_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFS4445913.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            21530..21769
                     /locus_tag="ACHY5W_000977"
     CDS             21530..21769
                     /locus_tag="ACHY5W_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4445914.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQETIKVRLDE"
     gene            complement(21770..22234)
                     /gene="nrdG"
                     /locus_tag="ACHY5W_000978"
     CDS             complement(21770..22234)
                     /gene="nrdG"
                     /locus_tag="ACHY5W_000978"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFS4445915.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22424..24562)
                     /gene="nrdD"
                     /locus_tag="ACHY5W_000979"
     CDS             complement(22424..24562)
                     /gene="nrdD"
                     /locus_tag="ACHY5W_000979"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4445916.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24956..26611)
                     /gene="treC"
                     /locus_tag="ACHY5W_000980"
     CDS             complement(24956..26611)
                     /gene="treC"
                     /locus_tag="ACHY5W_000980"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4445917.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26661..28082)
                     /gene="treB"
                     /locus_tag="ACHY5W_000981"
     CDS             complement(26661..28082)
                     /gene="treB"
                     /locus_tag="ACHY5W_000981"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFS4445918.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28201..29148)
                     /gene="treR"
                     /locus_tag="ACHY5W_000982"
     CDS             complement(28201..29148)
                     /gene="treR"
                     /locus_tag="ACHY5W_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFS4445919.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29333..29386
                     /gene="mgtL"
                     /locus_tag="ACHY5W_000983"
     CDS             29333..29386
                     /gene="mgtL"
                     /locus_tag="ACHY5W_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFS4445920.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29527..32223
                     /gene="mgtA"
                     /locus_tag="ACHY5W_000984"
     CDS             29527..32223
                     /gene="mgtA"
                     /locus_tag="ACHY5W_000984"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4445921.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVTPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32429..32815)
                     /gene="ridA"
                     /locus_tag="ACHY5W_000985"
     CDS             complement(32429..32815)
                     /gene="ridA"
                     /locus_tag="ACHY5W_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFS4445922.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32888..33349)
                     /gene="pyrI"
                     /locus_tag="ACHY5W_000986"
     CDS             complement(32888..33349)
                     /gene="pyrI"
                     /locus_tag="ACHY5W_000986"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFS4445923.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33362..34297)
                     /gene="pyrB"
                     /locus_tag="ACHY5W_000987"
     CDS             complement(33362..34297)
                     /gene="pyrB"
                     /locus_tag="ACHY5W_000987"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFS4445924.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34301..34435)
                     /gene="pyrL"
                     /locus_tag="ACHY5W_000988"
     CDS             complement(34301..34435)
                     /gene="pyrL"
                     /locus_tag="ACHY5W_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFS4445925.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34715..35110)
                     /gene="yjgH"
                     /locus_tag="ACHY5W_000989"
     CDS             complement(34715..35110)
                     /gene="yjgH"
                     /locus_tag="ACHY5W_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS4445926.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDITSFHTDPEKQFEDIMTVKNEIFSTP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(35241..35954)
                     /gene="bdcA"
                     /locus_tag="ACHY5W_000990"
     CDS             complement(35241..35954)
                     /gene="bdcA"
                     /locus_tag="ACHY5W_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4445927.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAESKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36025..36618
                     /gene="bdcR"
                     /locus_tag="ACHY5W_000991"
     CDS             36025..36618
                     /gene="bdcR"
                     /locus_tag="ACHY5W_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4445928.1"
                     /translation="MITKKQYRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            36763..37215
                     /gene="tabA"
                     /locus_tag="ACHY5W_000992"
     CDS             36763..37215
                     /gene="tabA"
                     /locus_tag="ACHY5W_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFS4445929.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37338..>37547
                     /locus_tag="ACHY5W_000993"
     CDS             37338..>37547
                     /locus_tag="ACHY5W_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4445930.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDF"
BASE COUNT         9042 a         9359 c        10100 g         9046 t
ORIGIN      
        1 gcagatagtg atattaccaa agaaaagatt tgcgagaaat ataaatcgac cattgaggcg
       61 tttaaaaaag agcaactagc gttaccagta ttagttcggc gtaagaactc agagacttaa
      121 ttcccctatc ccatagataa cgatagggga aaattatttt tgcagaattt atgttattgc
      181 cattatttga gcaacttttg cagcaggtcc atcacttgct gcacctcttc ctgagtgagc
      241 gccccgtctt tggcccattg cacgcgacca tccttatcca gcaccactac agcggaactc
      301 tcttcatcca gctgccaggc accgcgcgcg acgccattgc tatcgacaat aaactgcgac
      361 cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc ggaacccgga
      421 atcgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg taacttcgct
      481 gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg accagcaata
      541 tgttgcagta ctcgcacttt tcccactaac tgtgcgctgt tccaggtttt gtagctaaac
      601 tgatctttat caagcaccaa ctcgccccga tcggtaatgc caatcggcgg cactcgctga
      661 ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt gagtgccaga
      721 atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg accacttggg
      781 tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat gccagtttgc
      841 ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa
      901 aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat
      961 tgcccggata gtctatagtc actaagcatt aaaatttgcg cctcataata gttgggccga
     1021 ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt ttccaacggt
     1081 acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg ttatacatca
     1141 aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa gtgaaggata
     1201 agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg caaacagaga
     1261 tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag ccgttgatgt
     1321 ttctgggggt tacgcgacgc tttcttcttt atctttagag tcaggcagtt ttggcgaaag
     1381 ggctgtggcc ttgctgtcga tccgggtcac cagcagctgg tcgatgcggt agttatcgat
     1441 atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt
     1501 acgcagcata aacatcataa agccgccgat ggtttcgtag ttgcccgact gcgggaactc
     1561 gtcaatatcc agcacgcgca tgacgtcgtc aatcggggta ccgccgtcaa tcagccatga
     1621 gttctcatca cgggcgacaa tctgctcttc cagcccctga ccgaccagat cgcccatcag
     1681 cgtggtcatc acgtcgttga gggtgatgat ccccaccacc agcgcgtact cgttcatgat
     1741 caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa gggttaacgt
     1801 atccggcaca atcagcgtgt tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt
     1861 agccagcacg cggttcagca ggtctttaga atcgacatag ccgatgatgt ggtcaatatc
     1921 ttcattacag acgaggaact tagagtgcgg atgttccgcc accttattct tcagactttg
     1981 ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg acgacggaac
     2041 ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt tacgtaacac
     2101 gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga tgtcatcttt
     2161 acgtaccatt ggcagtttga aaatacggaa gattatgttc gccaggccgt tgaagaacca
     2221 caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac gcaaagccac
     2281 agcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca ggataaacat
     2341 gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagct ctgccgacat
     2401 atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc
     2461 gagaatcgcc actgcgttca gaccgatttg gaccacggta aagaacatgc cgggattttc
     2521 ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca gtttaagttt
     2581 gattttgcgt gaggcggcaa gcgagatctc ggacatcgag aagaacgcac ttacagcgat
     2641 caagcagagt atgactaaaa tactgtttaa catatcttat ccgacctttc aggccagatc
     2701 ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa
     2761 gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagccg ccagagggtt
     2821 aaaattcagg ctgctgataa atgcaaaact gcccgatgcg ctatgccgat caggcctacg
     2881 cagtccttgc aatatattga atttggaaga ttttgtaggc ctggcaaggc gtttacgccg
     2941 catccggcat aaacaaaacg cactttatca acaatctaca tttgtacccg taacgctatg
     3001 cttccggcgg cagacagacg ccaattccgc caattccaca gtaaccatac gggtttttat
     3061 gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta gcgatttccg
     3121 tggtgatgcg acgatcgtca tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg
     3181 cgcgagctgc ggcatcctgt tctggggtca gcggataaat cgctgaacga tactgcgtgc
     3241 cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa aatacctgta
     3301 gcaactgctc atagctgatg acggaaggat cgtaaacaat gcgtaccgct tcggcatgac
     3361 ccgtatcacc ggagcacact tcccgataag tcggatttgg cgtatagccg ccggtatagc
     3421 ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccaca ccccagaaac
     3481 aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt gagtgaccgt
     3541 taaccgcatg cagcgtggca acgggcatcg gggtgttacg tccaggcagg gcatcggcgg
     3601 gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga aaatcggtca
     3661 ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt gaataggtct
     3721 aaagtaaaac ataagaaata tttggggttt agtctgcttt ttaatccata ttactggatt
     3781 tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca ggagaaaatg
     3841 tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg
     3901 ccgcgaacgt ccgtctacag gtcgaggggg tatcgggaca gctggaaaag aacgttcgtg
     3961 cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg
     4021 tcgatgatgc catccgcgaa ggtctgaaag cgctgggtta ttaccagccg accattgaat
     4081 ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag
     4141 gcgtgccggt gttaattggc ggcactgatg tggtattgcg cggcggcgcg cggaccgata
     4201 aagactattt gaaattgctc gatactcgcc cggctattgg tacggtactg aaccagggcg
     4261 attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt tatttcgata
     4321 gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagct ttctgggata
     4381 ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa
     4441 tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag tacgaatcga
     4501 aagatctggc agaactgaac cgccgacttt ctgctaccgg ctggtttaac tcggtggtgg
     4561 tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt
     4621 cgccgcgaac agaaaacacc atcgaaaccg gggtcggtta ctctacggac gtgggaccgc
     4681 gcgtgaaagc gacgtggaaa aagccgtgga tgaactctta tggtcacagt ctgaccacca
     4741 gtaccagtat ttccgcgccg gaacagaccc tcgacttcag ctataaaatg ccgctgctga
     4801 agaatccact ggaacaatat tatttggtgc agggcggttt taagcgcact gacctgaacg
     4861 atacagagtc tgactccact acgctggtgg cttctcgcta ctgggatctc tccagcggct
     4921 ggcagcgtgc cattaacctg cgctggagtc tcgaccactt tactcagggt gaaattacca
     4981 acaccacgat gctgttttat cctggggtga tgattagccg cacgcgttct cgtggtggcc
     5041 tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac acggcctggg
     5101 gttcagatgt cgatttctcc gttttccagg cacaaaacgt ctggatccgc acactgtacg
     5161 atcgccatcg ttttgttaca cgcggcacgc tgggctggat tgaaaccggt gatttcgaca
     5221 aagtaccgcc ggatctgcgt ttcttcgccg ggggcgaccg cagtattcgt ggctacaaat
     5281 acaaatctat cgctccgaaa tacgccaacg gtgacctgaa aggggcctcg aagttgataa
     5341 ccggatcgct ggaataccag tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg
     5401 atagtggcga agcggtaagc gatattcgcc gcagcgactt taaaaccggt accggggtcg
     5461 gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg
     5521 ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta tgagtttatg
     5581 gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat cggtggcgtt
     5641 tctggtgggc accaccagcg gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc
     5701 aggactggat attggcaagg tcatcggcgg ctggcgcgat ctcaccttgt ctgacgttcg
     5761 ttatgagcag ccaggcgtgg cggtaaaagc gggcaatctg catctggctg tcgggcttga
     5821 gtgcctgtgg aacagtagcg tttgtattaa taacctggcg ctgaaagaca ttcaggtcaa
     5881 catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag atagcggtcc
     5941 gctggatctc tccacgccgt atcccatcac cctgacacgg gtggcactgg acaacgtcaa
     6001 catcaagatt gatgacacca cggtgtcggt gatggacttc acctccgggc tgaactggca
     6061 ggagaaaacc ctgaccctga aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa
     6121 agtggcggaa gtggcgcagg aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga
     6181 tgaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat tgccggaaat
     6241 gaccgacgtg catttgccgc ttaacctgaa cattgaagag tttaagggcg agcagctgcg
     6301 cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga gcagcattga
     6361 cggcaatact aaactggacg ccctggatat cgattccagt caagggatcg tcaacgccag
     6421 cggcacggcg cagctgtcag acaactggcc ggtggacatc actctcaaca gtacactgaa
     6481 cgtggagccg ttgaaaggtg aaaaagtgaa gctaaaagtg ggcggcgcgc tgcgcgaaca
     6541 gctggagata ggcgttaacc tttccggtcc ggtggatatg gatttacgcg cccagacgcg
     6601 actggcggaa gccggattgc cgctcaacgt ggaaataaac agcaaacagc tttactggcc
     6661 gttcactggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat
     6721 gaccgattac acgctctcta tgcgtacggc agtgaaggga ctggagatcc cgccagccac
     6781 cattaccctt gatgccaaag gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc
     6841 ggcgctggaa gggaaaactg aactcaaggc gttgctcgac tggcaacagg ccattagttg
     6901 gcgcggtgag ctaacgctta atggcattaa caccgccaaa gagttcccag agtggccgtc
     6961 gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct ggcagatgga
     7021 ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg ttgacggcac
     7081 gctgaaaggc aacagttata tgcagtggat gatccctgga ctgcatctgg aactggggcc
     7141 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
     7201 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga aagggctggt
     7261 gaaagttcgc ggcacggtgg aagcgccaca actactggcg gacatcaccg cgcgcggcct
     7321 gcgctggcag gagctaaccg tggcgcaggt tcgcgtggaa ggcgatatca aatccaccga
     7381 tcagattgcg ggtaaactcg acgtgcgcgt tgagcaaatt tcacagccgg atgtaaatat
     7441 caacctcgtc accctgaatg ccaaaggcag cgaaaagcag cacgagctac agttgcggat
     7501 tcagggcgag ccggtttccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
     7561 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
     7621 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
     7681 ctgttggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg cgggggccga
     7741 agggcgtgcg gtggtgaatc tcaaccgctt cgacctggcc atgctgaaac catttatgcc
     7801 agaaaccact caggccagcg gtatcttcac gggtaaagcg gacgttgcct gggacacaac
     7861 caaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca
     7921 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagacg ttgaatctgt cggcggagtt
     7981 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
     8041 tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
     8101 ccgtaacttc aaccttgcga tgataaaccc catctttacc cgtggggaaa aagcagcggg
     8161 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca
     8221 gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata tgcaaccgag
     8281 ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gccggtacag tacggaccca
     8341 gcagggtgaa atctacctga acggtgatgc cgactggagc caaattgaaa actggcgggc
     8401 gcgggtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga
     8461 tgtatcacca gatgttgtat tcgaggctac accaaacctg tttaccctcg atggtcgcgt
     8521 ggatgtcccg tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc
     8581 cagcgatgtg gtgatgctta acgataacct gcaaccagaa gaggcaaaaa cggcgtcgat
     8641 tccgattaac agtaacctga ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg
     8701 cctgaaagcg cggctgacgg gcgatctcaa tgtcgttcag gacaaacaag ggctgggcct
     8761 gaacgggcag atcaacatcc ctgaagggcg cttccatgca tatggtcagg atctgattgt
     8821 gcgtaagggt gagctactgt tctctggtcc gccggatcaa ccgtacctta atatcgaagc
     8881 tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagtttgcg tcactggtct
     8941 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac aggctgcatt
     9001 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg cggcgatgac
     9061 ctcgatgctg attggtctgg gggttgcgca aagtggccag attgtgggta aaatcggcga
     9121 gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact cctcccaggt
     9181 ggtggtcagc ggctatgtat tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga
     9241 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
     9301 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
     9361 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
     9421 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
     9481 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgacaa cgccacgctg
     9541 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
     9601 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
     9661 gaaagcggcg actggttaga cagggataag taataataaa agcacgccac cttcgggtgg
     9721 cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcacct tttgcttttc
     9781 tctccgagcc gctttccata tctattaacg cataaaaaac tctgctggca ttcacaaatg
     9841 cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac ttaaggaggt
     9901 gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca ttcccaatat
     9961 cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaagcgcagg tgagcaacaa
    10021 ccaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc tggcacagtg
    10081 tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg
    10141 tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct ggttggcttt
    10201 gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt
    10261 caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg cggaaatttt
    10321 gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt gcacggcgtg
    10381 gtgctggtta atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa gcgtattggt
    10441 ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt ggtggaataa
    10501 ggtgtgttta tttatcgcgg gcataaaaaa acccttacta accgaagccc ggcgttcagg
    10561 gttattgcgc tataagaact tatttattct tggcgcgctc gaaggaggca acgatttcag
    10621 ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct ttttcgaggt
    10681 ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggt agatcgttaa
    10741 cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca accagtttcg
    10801 catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa cggatcacag
    10861 aacccggctg cagcgggtac ggagtcggga ccagtacgtc aactgggtca ccgtccagag
    10921 acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg gacatgaagc
    10981 ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga tctgcgttag
    11041 ccgggatctc aataacaacg tagatgtctt ccggcagatc tttacccgca gggacgttga
    11101 gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag tattatagcc
    11161 aactcgcgcc gaatgtcttc gcttgttttc gtcttcattt tcctttttca ccagttttaa
    11221 gacggtatac agagcaggaa aatccataac tgtagccgca tttttcatag tgagatgaaa
    11281 gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat gtaacgcaat
    11341 ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca ataataatta
    11401 ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag tctcggcagc
    11461 catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg tcggatcgga
    11521 atccggctgg cgcgccgcag aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat
    11581 tacgctgaaa attgccgatg gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc
    11641 cttcgttgca caaggggtgg atgcgatctt tatcgctccg gtggtcgcga caggttggga
    11701 accggtatta aaagaggcga aagatgctga aatcccggtc tttttgctcg accgttccat
    11761 cgatgtgaaa gacaaatctc tctatatgac caccgttact gccgacaaca tcctcgaagg
    11821 caagttgatt ggtgactggc tggtaaaaga agtgaatggc aaaccatgca acgtggtgga
    11881 gctgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc
    11941 cattaagaat gcgccaaata tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag
    12001 taaaggcaaa gaagtcatgg agagctttat caaagcggaa aacaacggca aaaacatctg
    12061 catggtttac gcccataacg acgacatggt gattggtgca attcaggcaa ttaaagaagc
    12121 gggcctgaaa ccgggcaaag atatcctcac gggttccatt gacggtgtac cggacatcta
    12181 caaagcgatg atggatggcg aagcgaacgc cagtgttgaa ctgacgccga acatggcggg
    12241 acccgctttt gacgcgctgg agaaatacaa aaaagacggc accatgcctg aaaagctgat
    12301 gttaaccaaa tccacccttt acctgcctga taccgcaaaa gaagagttag agaagaagaa
    12361 aaatatgggg tattgagggt tgctatgcct gatgccgatt cgtaggccgg ataaggcgct
    12421 cgcgccgcat ccggcgatgg tgcactgaag cctgatgcga cgcttaccgc gtcttatcat
    12481 gcctactggg agcacgcttt acaccggggg aaaccatgac gaccgaccaa caccaggaaa
    12541 tcctccgcac cgaaggatta agtaaatttt tccccggcgt caaagcgtta gacaacgttg
    12601 atttcagcct gcgccgtggc gaaatcatgg cgctgctcgg tgaaaacggg gcgggaaaat
    12661 cgacgctaat caaagcatta accggtgttt accacgccga tcgcggcacc atctggctgg
    12721 aaggccaggc tatctcaccg aaaaataccg cccatgcaca acaactcggc atcggaaccg
    12781 tttatcagga agtcaacctg ctacccaata tgtcggtcgc tgataatcta ttcataggtc
    12841 gcgaacccaa acgtttcggg cttctacgcc gtaaagagat ggaaaagcgc gccaccgaac
    12901 tgatggcatc ttacggtttc tccctcgacg tgcgtgaacc gcttaaccgc ttttcagtcg
    12961 cgatgcagca aatcgtcgcc atttgccggg ctatcgatct ctctgccaaa gtgctgatcc
    13021 tcgatgaacc caccgccagt ctcgacaccc aggaagtgga gttactgttt ggcctgatgc
    13081 gtcagttgcg cgatcgcggc gtcagcctga tctttgtcac tcactttctc gatcaggtct
    13141 atcaggtcag cgatcggatc accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa
    13201 cgcgcgagct accgcagatc gaactggtaa aaatgatgct ggggcgcgag ttggataccc
    13261 acgcgctaca gcgtgccggg cgaacattgt tgagcgacaa acccgttgca gcgttcaaaa
    13321 attacggcaa aaaaggaacg atcgcaccgt ttgatctcga agtacgcccc ggcgagatcg
    13381 tcggtctggc tggcttgctg ggatcaggac gtaccgaaac cgccgaagtg atcttcggta
    13441 tcaaacctgc tgacagcggc actgcgttga tcaaaggcaa acagcaaacc ctgcgatcgc
    13501 cacatcaggc ttcggtactg ggcatcggct tctgcccgga agacaggaaa accgatggca
    13561 tcatcgccgc cgcctcggtg cgggaaaata tcatcctcgc tctccaggcc cagcgcggct
    13621 ggctacgacc catttcccgc aaagagcagc aagagattgc cgaacgcttt atccgccagc
    13681 ttggcattcg cactccttca actgaacaac cgattgaatt tctctccggc ggcaatcagc
    13741 aaaaagtgtt gctttcacgt tggctactga cccgaccgca atttctgatc cttgatgagc
    13801 caacccgcgg cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt gaaacgctat
    13861 gcgccgatgg tctggcgctg ctggtgatct cctccgaact ggaagagctg gtgggctatg
    13921 ccgatcgggt gatcatcatg cgcgatcgca aacaggtggc ggagatcccg ctggcagagc
    13981 tttccgttcc ggcgatcatg aacgccattg cggcgtaagg agaacagtgt gatgcctcaa
    14041 tctctcccgg acactacgcc gccgaaaagg cgctttcgct ggccaacggg aatgccgcag
    14101 ctggcggcac tgttgctggt gctgctggtc gatagcctgg tggccccgca tttctggcag
    14161 gtggtgcttc aggatgggcg tttgttcggt agccctatcg acattcttaa ccgtgcggcc
    14221 cccgttgcgc tgttggcgat tggcatgacg ctggtgatcg caacaggtgg gattgatctc
    14281 tctgtggggg cggtgatggc tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga
    14341 ttcagcctgc cgattgtttt gttaagcgcc ctgggcactg gcatcctggc gggattgtgg
    14401 aacggcatac tggtagcgat cctcaaaatt cagccgtttg tcgccactct gatcctgatg
    14461 gtcgccgggc gcggcgtggc gcaactgatc acctccgggc agatcgtcac gtttaactcg
    14521 ccgcatctct catggtttgg cagtggatcg ctattgttcc tgccaacacc ggtcattatc
    14581 gcggtgctga cgcttctcct gttctggctg ttgacccgca aaacggcgct ggggatgttt
    14641 atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa cacgcgaatc
    14701 atcgtcatgc tcacctacgt gttgagcggg ctatgtgcgg cgattgcggg cattatcgtg
    14761 gcggcggata ttcgcggtgc cgatgccaac aacgccggat tatggctgga gctggacgcc
    14821 attctcgcgg tggtgattgg cggcggatcg ctgatgggcg ggcgttttaa cctgctgctc
    14881 tcagtggtag gggcgctgat tattcaggga atgaacaccg gaattttgct ttcgggcttt
    14941 ccgccagaga tgaaccaggt ggtgaaagcg gtagtggtgc tttgcgtgct gattgttcag
    15001 tcgcaacgct ttatcagtct gattaaagga gtgcgtagcc gtgataaaac gtaacctacc
    15061 gcttatgatc accatcggcg tctttgtgct gggttatctc tactgtctga cgcagtttcc
    15121 cggttttgct tccacaagag tgatctgcaa tatcctgacc gataacgcct ttcttgggat
    15181 cattgccgtt ggcatgacct ttgtgatcct ctccggtggg atcgatctct ccgtcggttc
    15241 ggtgatcgac tttactggcg tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc
    15301 gctgctggcg tttccgctag tgctggggat gggctgtgcc ttcggcgcat ttatggggct
    15361 tctgatcgat gccctgaaga tcccggcatt tatcattacg cttgcgggga tgttcttttt
    15421 gcgcggcgtc agctatctcg tttcggaaga gtcgattccg ataaaccatc ccatttatga
    15481 cacgctctca agccttgcgt ggaaaatccc tggcggcggt cgcttaagtg cgatgggact
    15541 gctgatgctg gcggtggtgg ttatcggcat attcctcgcg catcgtaccc gttttggtaa
    15601 tcaggtgtac gccattgggg gcaatgcgac gtcggcaaac ctgatgggga tttccactcg
    15661 cagcaccact attcgcattt atatgctctc caccggactg gcaacgctgg cggggattgt
    15721 cttctcgatt tatacccagg ccggatatgc gctggcgggc gtaggtgtgg aactggatgc
    15781 tatcgcctca gtggtgattg gcggtacgct tttgagcggt ggcgttggaa cggtattagg
    15841 gacgcttttt ggcgtggcga ttcagggact gattcagact tacataaact ttgatggcac
    15901 gctgagctca tggtggacga aaatcgccat cggtattttg ttgtttattt ttatagcatt
    15961 acagcgtgga ttaacggtac tgtgggagaa tcgtcagagt tcgcctgtga caagagtcaa
    16021 cattgcgcag caataaaaac gcctctccgt gtggagaggc gcaggagatt acgcgtccgg
    16081 gaactcacgg ataaagcgtt cgacatcttc aaccatatgg tcgttgccga caaagaacga
    16141 acggcgctgg tgcagagttt ccgggatgat atccagaata cgctctttgc catcgctcgc
    16201 tttaccgccc gcttgttccg ccaggaatgc catcgggttg cactcataca gtaaacgcag
    16261 tttaccgtcc gggtggctgg cggtgcttgg gtagagataa ataccgcctt tcagcaggtt
    16321 acggtggaaa tccgcgacca gtgaaccgat ataacgtgag gtataagggc ggttggtgga
    16381 cttatcttct tcctggcaga atttaatgta cttcttcacc ccgttcggaa acttaatgta
    16441 gtttccttcg ttgatggagt aggttttgcc tttctccggg aagcgcatcc gttcctggca
    16501 caggcagaaa acgccgagcg aaggatcgta agtaaaggcg tgaacaccgc atccggtggt
    16561 gtaaaccagc atggtagagg agccgtatac cacgtaacct gccgcaacct gtttgttacc
    16621 aggctggagg aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc ggcggtagat
    16681 ggagaaaatg gtaccgacag agacgttaac atcgatgttg gacgagccat ccagtgggtc
    16741 catcagcacc acgtattttg catgttcaca gccttcaaag acaacaatct catcttcttc
    16801 ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt gcggctttca gtttttcatt
    16861 agcgaacaag tcgagtttct gctgaacctc gccctgcacg ttctcagcac cgctggcacc
    16921 caggatatca accagtcctg ctttgttgat atcgcgatgg ataatcttgg cgcccagttt
    16981 tattgccgac agcaaagcag tgagctcacc ggtagcatga gaaaactcgt gctgcttttc
    17041 gacaataaat tcacctaacg ttttcataaa actttccctg caatgtttat ggagtaaagc
    17101 gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa
    17161 ttacggatta tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca
    17221 gattaaaaaa ggatagtgac gtatgcgcat tcatatttta ggaatttgtg gcacgtttat
    17281 gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa
    17341 tgtgtatccg ccgatgagca ccttacttga gaagcaaggc attgaactga ttcagggtta
    17401 cgatgccagc cagctcgatc cgcagccgga tctggtgatt attggcaacg ccatgacccg
    17461 tggaaatccg tgtgtggaag cggtactgga aaaaaacatc ccttatatgt caggtccaca
    17521 gtggctgcac gattttgtgc tgcgtgaccg ctgggtgctg gccgttgccg gtacacacgg
    17581 caaaactacc accgcgggaa tggcgacctg gattctggaa cagtgcggtt acaaaccggg
    17641 atttgtgatc ggcggtgtgc cggggaattt tgaggtttcg gcgcgtctgg gcgaaagcga
    17701 cttctttgtt atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac gttctaaatt
    17761 tgttcattac tgcccgcgta cgctgatcct caacaacctt gagttcgatc acgccgatat
    17821 ctttgacgac ctgaaagcga tccagaaaca gttccaccat ctggtgcgta tcgttccggg
    17881 gcagggccgt attatctggc cggaaaacga catcaacctg aaacagacca tggcgatggg
    17941 ctgctggagc gagcaggagc tggtgggcga gcagggtcac tggcaggcga aaaagctaac
    18001 cactgacgct tctgaatggg aagtcttgtt ggatggcgaa aaagtgggcg aagtgaaatg
    18061 gtcgctggta ggcgaacata atatgcacaa tggcctgatg gcgattgcag cggctcgcca
    18121 tgttggtgta gcgccggcag atgccgctaa cgcgctgggt tcgtttatta atgctcgtcg
    18181 ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta tatgacgatt ttgcccatca
    18241 cccgacggcg attctggcaa cgctggcggc gctgcgtggc aaagttggtg gtacggcgcg
    18301 cattattgct gtgctggaac cgcgctcgaa taccatgaaa atggggatct gcaaagacga
    18361 tttggcacct tcattaggtc gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc
    18421 gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct gcacactgga gtggcgatgt
    18481 ggatacgctg gcagatatgg tggtgaaaac cgctcagcct ggcgaccata ttctggtgat
    18541 gagcaacggc ggttttggtg ggatccatca gaaactgctg gatgggctgg cgaagaaggc
    18601 ggaagccgcg cagtaattcg gccttagcca gatatagtgt gcgtaggccg gataaggcgt
    18661 tcacgccgca tccggcattt gagcatagtg cctgatgcga cgcttgatgc gtcttatcag
    18721 gcctacaggt gcaccgtatc cggcaaacca tactcacatc aacaacgaaa attacccttc
    18781 gttctccgct aactcgcgca gatactggaa aatctggcgt gcggatttcg gcggcttatt
    18841 cccttctttc tctttcttcg cgttgcggat cagagtacgc agttgctgac gatcggcatc
    18901 gggccacaga tttaatacct cagcgatggc gtcatcacct tgatcgatca gacgatcgcg
    18961 caggttttcc agtttatgga acagcaccac ctgctggttg tgacggtttt tcagcttatc
    19021 cagcgcctga cgaataggct ctacgtcgcg ctggcgcagc atcttaccaa tgagctgcaa
    19081 ctggcggcgg cgaccttcca tcttaatacg ctgggcgagc tcaatagccg cgcgcagatc
    19141 cgcatctaac gggatcttat ccagcgcgtt tttccccaga tcaacaattt ccgcgccaag
    19201 gcgttttagc tcctcggcat cacgtttaat ttcactttta ctgacccaga taatttcatc
    19261 gtcttcgtct tcgatgtcat caccgggaac gtcgtcgagc cagtcttcgg gctgcttagt
    19321 catctcaggc tccttaaaaa aagaggctaa tgttaccagt taagatgcgc actgaaaaac
    19381 ggttctctgt tagacttcag agaaactctc tacattatgg cacttgcaat gaaagtaatc
    19441 tctcaagttg aagcgcagcg caagattctg gaagaagcag tttccactgc gctggagttg
    19501 gcctcaggca aatcggacgg tgcggaagtt gccgtcagca agaccaccgg cattagcgta
    19561 agcacgcgtt atggtgaagt ggagaatgtt gaattcaata gcgatggcgc gctggggatc
    19621 actgtttatc accagaaccg caaaggtagc gcatcatcca ccgatttaag cccgcaggcc
    19681 attgcccgta ctgtacaggc ggcgctggat attgcccgtt atacctcgcc agatccctgt
    19741 gccggcgtgg cagacaaaga gctgctggcc tttgacgcac cagatctcga cttgttccac
    19801 cctgcggaag tttccccgga tgaagccatt gaactggcgg cccgcgcaga acaggcggca
    19861 ttgcaggcgg acaaacgcat caccaatacc gaaggtggca gctttaacag ccactacggt
    19921 gtcaaagttt ttggcaacag ccacggcatg ttgcagggtt actgctcaac gcgtcattcg
    19981 ctctccagct gtgtaattgc cgaagaaaat ggcgatatgg agcgtgatta cgcctacacc
    20041 attgggcggg cgatgagcga tctgcaaacg ccagagtggg tcggggccga ctgtgctcgc
    20101 cgcactttat cgcgcctgtc accgcgaaaa ctctccacca tgaaagcgcc agtgattttt
    20161 gctaatgaag tggcaaccgg gctgttcggt catctggttg gcgcaattgc aggcggttcg
    20221 gtttaccgta aatctacctt cctgctggat tcgctgggta aacaaattct gccggactgg
    20281 ctgaccattg aagagcatcc gcatctgctg aaagggctgg cgtcgacgcc attcgacagc
    20341 gaaggtgtgc gcaccgagcg tcgcgatatt attaaagatg gcatcctgac tcagtggctg
    20401 ctgaccagct attcggcgcg aaaactgggg ctgaaaagca ccggacatgc gggcggtatt
    20461 cacaactggc ggattgccgg acaaggtcta agcttcgagc agatgctcaa agagatgggc
    20521 accgggctgg tggtgacgga attgatgggc cagggcgtga gtgcaattac cggtgattat
    20581 tcccgtggtg cggcgggctt ctgggtagaa aacggcgaaa ttcagtatcc ggttagcgaa
    20641 atcaccatcg caggtaattt aaaagatatg tggcgcaata ttgtcaccgt cggtaacgat
    20701 attgaaacac gcagtaatat acagtgtggt tctgtgctgt tgccggagat gaaaatcgcc
    20761 ggacagtaaa aaaactggcg cgacctgccg cgctataaac taaaaaatta cacaaaatca
    20821 ttcgcactgc atcgagacgg aaagtgaatg agggccgggg agcgtactcg cagtacgtga
    20881 cccggtcgaa tgagcgtagt cgtcgatgag gcagtgtgaa ggatgaagtg taaataaaaa
    20941 aggaagtgag caatgcgaaa aagcctgtta gctattcttg cagtctcctc gttggtattc
    21001 agttcggcgt cgtttgccgc tgatcttgaa gacaatatgg aaaccctcaa cgacaattta
    21061 aaagtggtcg aaaaagccga taacgcggcg caagtcaaag acgcgttaac gaagatgcgc
    21121 gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc tcgaagataa atcaccggac
    21181 agcccggaaa tgaaagattt ccgccacggt ttcgacattc tggtcggtca gattgacgac
    21241 gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc aggctgctgc agagcaactg
    21301 aaaacgaccc gcaattcata tcacaagaag tatcgttaat tccttattgg gcggtgaaac
    21361 gttaccgccc aacgctttct tacccctcgt aaaatcaccg ccaaatctct cttcactacg
    21421 gcatatttct gcgtgtcgac gccatttttg cgaggcgttt tccagaaatg aatgtaatgc
    21481 atatgtaatg ctataataca ttacactttt cataattacc ggaggcataa tggccacact
    21541 taacgtccgc ctggatgaca aactgaaaaa tgaggcttat gcggtgctgg aaaaattaaa
    21601 tatcacgcct actgaagcag tgcggctgct gtttcagtac gttgcggaaa atggacgaat
    21661 gccggtaaaa acgctcactg tcagtgatag tgaagaagcc ttgttacgca ctgtcaggga
    21721 acgactggcc aacccacaag agaccattaa ggtccggctg gatgagtgat taacgcaaat
    21781 gatgcaccac ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca
    21841 caaatttgcc gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga
    21901 gttcgtcgag tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga
    21961 tgcgttgtac cagtttcaga atatccggca cgttttgcgg atgcagcggg tcgccgccgg
    22021 agagggaaat accctggcgt ttgatacgag tgtcattcag atcgttaatg atctggtctt
    22081 ccattgcttt ggtaaatggc tgaccggaat ttacccgcca ggtgcttttg ttatagcaac
    22141 cggggcattc atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga
    22201 cgatgtcgac aggatagtac tgatgataat tcattgtttc gcctggctta tcagtccgcg
    22261 tataggatgc atccggtaat caacgcctga tgcgacgctg tcgcgtctta tcaggcctgg
    22321 agcagttgcc aaatgtaggc cggataaggc gtttacgccg catccggcaa tcaacgcggg
    22381 atgcgacgct ttcacatcac atccggcatt tgcttagtgc agatcaacct atctgcccat
    22441 ttcccaaatg tttaacgcgg cgcttaactt cttcctgctt accggcgtta aacggacgtg
    22501 catccgggct acctaaatat ccgcacacgc ggcgagttac cgacacacgg gaggcgtcat
    22561 ggttaccaca tttcgggcaa gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc
    22621 cacactcgta gcactcatca atcggtgtat tggtgccgta atacggtaca tgctgatagc
    22681 tgtaatccca gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccgt
    22741 agcaaatgaa accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt
    22801 acgggttcac cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg
    22861 tcacgcccgg caccacgcca aactcagcag tatcgagacg gcagaagcga tcgcacaggt
    22921 tttcactcgg cgtgctgtag agactgaaac cataacccgt ttcttctttc cactgatcca
    22981 ctgcctgacg cagacgttca acaatggcga tacctttcgc gcgaagctgc tcgttgtcgt
    23041 aaacatgctc gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg
    23101 aaatagacgc acgaccgttt ttgaagattt cagaaacatc atcgtcagca ttcagacgca
    23161 cgccacaagc accttccata tagaggatcg gggccacgcg cgctttcacg ccttcgagac
    23221 gagcgatacg ggtcatcagc gccttacgtg ccagcaccag acgttcatcc agcagcttcc
    23281 agaaggtggc ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca
    23341 cgccgaggtt gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga
    23401 ggaagctgcg gcagcccatc ggagttttaa acgaaccggt gactttcact acctgatcgt
    23461 agttcaggat atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt
    23521 cgtagttcgg atcgcctttt ttatggttca ggccatcgcg aatcgcaaac accagtttcg
    23581 ggaacaccgc agttttacgg tttttaccga ggcctgcgat acggttacgc aggatagatt
    23641 cctgaatcag gcgcgactcc cagctggtgc ccagaccaaa accaaaggtt acaaacggcg
    23701 tctgaccgtt ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt
    23761 agcactcttt aatggtacga gagttagcgt agccttcggc gtccgggatg ttccactctt
    23821 ctgcggtctt gcgatgtttg ttgtagctgg cggtgacaaa cggtgccagc acttcatcga
    23881 tacggttaat ggtggtgccg ccataaatat ggctggcgac ctgagcaata atctgcgcag
    23941 ttaccgcggt tgccgtagag atcgacttcg gcggttcaat ctcggcgttc cccatcttaa
    24001 agccctgggt cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaagaacg
    24061 gcgagtaatc gagatcgtga tagtgaatat cgccacgctc atgtgcctgc accacgtcac
    24121 gcggcagcag gtgctgacgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct
    24181 gggttggaat caccttgctg tctttgttgg cgttttcgtt gagtaacgag gcgttggtct
    24241 gctcgaccag accacggatc tcctggttca ggcgaccgcg tttttcacgt tcaatgtcgc
    24301 gatcgtgacg gtactcgatg taagcacgag ccagttgttt gtatggacct gacatcagct
    24361 ggttttcaac agcggtctgg atctcattga tatccacctg gttgcggccc tgcatctgct
    24421 cgctgacaac cgcagcaaca gtggcgcaat aatctgcatc atcgacttcc gctgctttag
    24481 ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc
    24541 gtttcatcac atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa
    24601 attgggaaag gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca
    24661 tccgcttatc cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga
    24721 ttaatacaac atattgggtt gggatgcatt ttaaagtcta tatatagtgc tttgcatcaa
    24781 ggatgtttga actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg
    24841 cgctacagcg ttatgtaaat tttttaatga atttgctggt tgaaaaatca acaaaaacaa
    24901 catgctgaca gacaaaaccc cggaatgaca ggcattaccg gggcttagcg aagatttact
    24961 tctgtaacca ccagacagcc tcaaaaggcc gtagagtcat ggcacagggt tgtggtgagg
    25021 cttcttcgta gttatgcatc acaagctgcc agttgccgcg catttgccct ggctgccagg
    25081 gttggatctc acggctaagg ttggcaatga ccagcaaggt ttgccccttc cattcacggc
    25141 gatagcacca caatacaggg ctgtttggca gcagatcctg gtaattgccc catgtcagga
    25201 tggcttcctg cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggaatcat
    25261 cggccaacgc ggcttctacg ttgatttgtt gatagttatc gcccaggcca atccacggtt
    25321 cgccagccgt aaacccggca ttatcgccgt tgctccattg catgggcgtg cggctgttgt
    25381 cacgggattt actggcgagg attgccaata actcgtcggc atcacgccca tcgttgcgca
    25441 gctcggcaaa catattgagg ctctctacgt cgcgatagtc agtaatgcgc gtgaaatgcg
    25501 ggttagtcat gccaatctct tcgccctggt agatatacgg cgttccctgc atgccatgca
    25561 gcaccatcgc cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag
    25621 aaacaatgcg cggctgatcg tggttacacc agaacaaggc attccatgcc acgttgtgca
    25681 ttccttgttg ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg
    25741 tccatttttc accaccggga taatcgacct tcaggtgatg aaaattaaat gtcatcgata
    25801 attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt
    25861 cacctacggt cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact
    25921 cgtgtgctcg tggcccgtcg gtgtagaagc gacgcccgtc gccgtccagg tcttcaggga
    25981 aacgcgggtc tttggagatc agattcacca catccaggcg caacccgtcg accccacgat
    26041 cggcccagaa ctcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga
    26101 gatccgcctg ttctggtgca aagagatgca aatagtactg ttcgctttcc gcatgccagc
    26161 gccacgcact accgccaaat tttgaacgcc agttgttcgg tggcgtttct ggttcgccat
    26221 cgcgccagat ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg
    26281 catgttgggt agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt
    26341 ttgcctgcgt caccagttcg tcaaaatcgt ccagcgtgcc gtaggtggga tcaatcgccg
    26401 tatagttcgc tacgtcgtaa ccgttatcga cctggggaga gacataaaag ggggttagcc
    26461 agatggcatc aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat
    26521 cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc
    26581 cgttttgcca ccagtgggga agattagtca ttacgttatt cctgcaaatg cgagggggcg
    26641 caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta tttccgctga
    26701 tagataaacg aggtgagtac aatcgggatg atgatggcga tagccattgc cagcgcaaac
    26761 acctgccagt agctcggttg aatcgagaga attcccggca ggccgcctac gccgatgcca
    26821 ttcgccataa cgccgttcag gccgcatagc aatcctgcca gaccagaacc aatcatcgcg
    26881 cacagcatcg ggaagcgata tttcaggttg atgccgtaca ttgcaggctc agtgacccca
    26941 agccaggcgg agatcgccgc aggcacggag atctcgcgtt cattgtgttt gcggctggaa
    27001 atgataatgc ctatcacggc ggagccctga gcgatattcg acagcgcaat cagcggccac
    27061 actggcgtac cacccatgct ttgaatcatc tgcaaatcaa tagcaagcgt ggtctggtgt
    27121 accccggtga tcaccagcgg ggcgtacagg aagccaaaca atgccgcgcc aatcggagca
    27181 aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca
    27241 aacggaccaa tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc
    27301 accaccagat agaggtaatc tggcacgatg cgtttaaggc gagtttcaat aacgcccagc
    27361 gccagcccgg ctaacagtgc cgggatcacc tgcgcctgat agcccacttt ggcgatgctg
    27421 aacatgccaa agtcccacac ttccggcagc tgctgcccga gcagataagc gttcatcagc
    27481 tggggggaaa ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc gcccattttt
    27541 ttcaccgctg accagcaaat cccgaccggc aggtagaaga agatcgcttc accgatcaac
    27601 cacagaaaat cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ttgaccgttg
    27661 ctcatgggca aatcgccgat cacattgcga aaaccgagga tcaaaccgcc gctgatcaac
    27721 gcgggcagca acgggaagaa gatctccgcg aaatgagaga tcaactgctc atgccatttc
    27781 atattctgcc gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac
    27841 gctatcagtg cttgatagta atcacccacg ttggtgccaa tcaccacctg aaattgcccg
    27901 gcattggtga aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg
    27961 gccggttggt tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg
    28021 ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc
    28081 ataaagcccc atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat
    28141 ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca atttatcgtt caggaaacga
    28201 tcaggacagg gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgcgc
    28261 gatcaactgg caggccgcct ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat
    28321 ctccggatga aggaatttca ttaatggcgt attaccgacg ctcgccagtt gcaaggtatc
    28381 gatgcgttgt tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag
    28441 taaggcagta gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc
    28501 aaggcctggc agggcggcaa cgggatgcag tttatgcgct ttgcagaacg ccaggtaggc
    28561 ttcgtgacgt cgcttcccgg tcgtcacgtc actatgcggc acgccgagat aactgatatt
    28621 gcgatgcccc tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata
    28681 acagaccgaa gcaaagcctt ttgcgtcacg cgccaacaga accagcgatg actgccagtg
    28741 ggctaacatt tcttctgtta tgccggtaaa accgaacagc actacgccgt cgatattacg
    28801 ccgtttcagc acccccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat
    28861 gattggatcg taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc
    28921 tgacaacgaa tccagacggg tgacaatgat ggcgaccact ttatcgcttt gcccacgcat
    28981 agcgcgcgca gagcgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc
    29041 gcgggtgcgc tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc
    29101 cacgccgctt aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg
    29161 taacgtgttg tttaattatt tgagcctaac tttacccgtg cattcagcaa tgggtaaagt
    29221 ctggtttatc gttggtttag ttgccagcag gtattatatc gccatagatg ctacgaatat
    29281 tattggattc tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc
    29341 tgatcccacg cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac
    29401 ggcgttaccg gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt
    29461 caggtaaggc ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga
    29521 ctccttatgt ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt
    29581 catcgtgatc cattgcctgg cgcgcagcag acagtgaata cggtggtccc gccgtcctta
    29641 agtgcgcatt gcctgaaaat ggcggtgacg cccgaagaag aattgtggaa aacgttcgac
    29701 actcatccgg aagggttaaa tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa
    29761 aataaattac ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc
    29821 aaccccttta atattttact caccattctc ggcgctattt cttacgccac ggaagattta
    29881 tttgccgcgg gcgttatcgc gctaatggtc gctatttcaa cgttgctgaa ctttattcag
    29941 gaagcacgtt ccactaaagc ggcagatgcc ctgaaagcga tggtcagcaa taccgcgacg
    30001 gtgctgcgcg taattaacga caaaggagaa aatggctggc tggagatccc gatcgaccag
    30061 ctggtgcccg gcgatattat aaaactggcg gcgggagata tgatcccggc agatttacgt
    30121 atcttgcagg cgcgggatct gttcgtcgct caggcgtcgt taaccggtga gtctctgccc
    30181 gtggaaaaag ccgctaccac tcgccagccg gagcacagca atccgctgga gtgcgacacg
    30241 ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgctaca
    30301 ggtgccaata cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg
    30361 aatgcctttc agcaagggat cagccgcgtc agtatgctgc tgattcgctt tatgctggtg
    30421 atggcaccgg tggtgctgtt aatcaatggt tacaccaaag gcgactggtg ggaagcggcg
    30481 ctgtttgcgc tttcggtcgc ggtaggccta acgccggaaa tgttgccgat gattgttacc
    30541 tcgacgctgg cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt caaacatctg
    30601 gatgctattc agaactttgg cgcaatggat attctgtgca ctgataaaac cggcaccctg
    30661 acgcaggata aaattgtgct ggagaatcat accgatatct ccggtaaaac cagcgaacgc
    30721 gtgctgcata gcgcgtggtt gaacagtcat tatcagaccg gacttaaaaa cctgctcgat
    30781 acagcggtgc tcgaaggtac ggatgaagag tcagcgcgct cgctggccag tcgttggcaa
    30841 aaaatcgatg agattccgtt tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa
    30901 aataccgagc accatcagct ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt
    30961 tcgcaggtgc gtcacaatgg cgagattgtg ccgctcgatg acatcatgct gcgtaagatt
    31021 aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa
    31081 tacctgccag cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa
    31141 ggatatattg cttttcttga tccgccaaaa gagacaaccg ctccggcact gaaggcatta
    31201 aaagcgagtg gaattaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa
    31261 gtgtgccatg aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg
    31321 ctatctgacg acgaactggc aaatcttgca cagcgcacca cgctgtttgc ccgcctgacg
    31381 ccgatgcata aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt
    31441 atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg
    31501 gacggcgcgg tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg
    31561 atggtgctgg aagagggggt tattgaggga cgtcgcactt tcgccaacat gctgaaatac
    31621 atcaaaatga cggcgagctc taacttcggt aatgtgttca gcgtgctggt ggcgagtgct
    31681 ttcttgccct tcctgccgat gttgccgtta cacttgctta ttcagaactt gctgtacgat
    31741 gtgtcacagg tggcgatccc gtttgataac gtcgacgacg agcaaattca aaagccgcag
    31801 cgttggaatc cggcggatct ggggcgcttt atgatcttct tcggaccgat cagctcgatc
    31861 ttcgatattc tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaaca
    31921 caaacgctgt tccagtcggg atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg
    31981 catatgatcc gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg
    32041 atcatgaccg tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt
    32101 tatctgcaat tacaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca
    32161 gggtatatga cattaaccca gttggtgaaa gggttctata gccgtcgtta cggctggcaa
    32221 taaagaataa actgggcacg atagccccga ttcagattgc agacaaagta agctttgttc
    32281 atgccggatg cggcgtaaat gccttatccg gccaacaaat tcaataaatt gcaggaatca
    32341 cgtaggcctg ataagcgtag cgcatcaggc gattttgcgt ttgtcatcag acttaatccg
    32401 ggcatgatag cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa
    32461 tcttcacgtc tttcggcaga cgggcaactt caacgcaaga acgtgccggg aaggtggcgt
    32521 tgtgttcggt gaagaaggct tcgtaagtgg cgtttacggt tgcgaagtcg ttcagatctt
    32581 tcacaaacac ggtggtttta acgatgtcgc ccactttcag gccagcggct tcgacgatcg
    32641 cttttacgtt atccagcgac tgacgtgcct gtgcagcgac gtctgccggt acttcgcccg
    32701 ttttcggatt taccgggatc tgaccggagg tgatgatcat attgcccaga tcaacgccct
    32761 gtacgtaagg accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt
    32821 ctcctttatt acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca
    32881 accgcaatta attggccagc accacattat gggaaaactc tttttcacag tatttgcatt
    32941 tgagcgcgat atcattggcg cgttttcgca cggcaaagct ggaagaaacc ggttcggcat
    33001 ggctgataca gttgctgttc gggcagacca gcacattgtc gatgcgctcc ggcagacttg
    33061 ggcgcgattt acccaccact tcatagttgt cgatacggtt aaccgtggct tgcggcgcat
    33121 acaatgccag ttgatctact tgatcttcac tcaaaaaggt attttcgatt ttgatcagat
    33181 ctttgcggcc catctcgcca gaaggcaggt tcagaccaat ggtgatgcgc tgatctgttt
    33241 cggtcagctt gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta
    33301 ccgtgccgcg tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc
    33361 cttacagtac cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc
    33421 cgttgcctgc ctgctggaag taccaggcgt gtggcgtttt atcaacatcc gtcgcaatct
    33481 catcaacacg cggcagcgga tgcagtactt tcatattggc tttggcgttg tggagatcgc
    33541 tggcgcgaag aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt
    33601 tttgcacgcg ggtcatgtac aggatgtcta cttccgccat cacttcttca atagagctgt
    33661 gcagactcca tgcgatccct ttttcatcaa gcatatccag aatgtattgc ggcattgcca
    33721 gcgcgtccgg cgcgatgaag taaaaacggt tgccgtcgaa cttcgctaac gcctgagtca
    33781 gggagtgaac ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca
    33841 gacgcccctg ggtttcctga atggtgaata agtccagcaa ggtttgcgtc ggatgttggt
    33901 tggagccatc accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg
    33961 ccgcaccttc ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa
    34021 tggtatcggc cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc
    34081 ccaccacgct ggcacccagg cggtgcatgg aagtttcgaa agagaggcgg gtacgggtag
    34141 aggcttcgaa gaaacagctg gcaatgactt tgtgcttcaa cagctctggt tgcgggtttg
    34201 ctttcagttt cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta
    34261 tggaaatgat atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc
    34321 aaaaaaaagc ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca
    34381 ggccagcgtc ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc
    34441 ctcccggcaa attgtccggc attatactca tcgtcagaag cggtgcaagc atttgatgca
    34501 atattttgtc agcgcaaacg gtttacttga attataagtc aaaatatatg catttttatt
    34561 tgatgtgatt ctgcaggcga ctgtcgttcg gtatcaataa ttgcagacat ttcctgcttt
    34621 tttctttttc actttcacgc aatcagatat gcattttatt ccattcatcc gacttatagg
    34681 gcggagtgtg aaaacgaacg gctaacacta ttgcttactg ctcagggatg cgcgctatca
    34741 ctttaatttc aaaatcaaag cctgccagcc atgtaacacc caccgccgtc cagtttggat
    34801 aaggtggggt gctaaatatt tcatttttca ccgtcatgat gtcttcgaat tgtttttctg
    34861 gatcggtatg aaagctcgta atatcaatga tattgtcaaa agtgcatccc gcagcagcca
    34921 gggtcgcatg caaattatca aatgccagtc tgacttgttg ctgaaaatcg ggttctggtg
    34981 ttccgtcctc tcgacttcct acttgcccgg aaacaaacag caaatcgccg gaacgaatag
    35041 ccgcagaata acgatgctca gcatacagtg aatgtcgtcc agcagggaaa acagcggttc
    35101 tttctaccat ttggttatcc tcaagattta cgacatgaac agaagattta tatttatcag
    35161 gagccgcttt tagcggacga cgtgagtaaa caaaacccag acatcatgga taatggctgg
    35221 gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg catcgcgccg
    35281 gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac ctcttccggt
    35341 tgcccatgtc ttttgatagc cataaaacta tgcaacatat cgcgcattgg cccgttggcg
    35401 ggattagcgt cggtatcaat tggccctggc tggacgacgt tgatggtgat cccacgcggt
    35461 ccgaaatcac gggccagccc gcgcgccatg ccttgcaggg cagatttact ggcggcataa
    35521 gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat taagatgcgc
    35581 ccgccttcgg gcatctgccg ggcggcttca acagaggcat ggtaaggagc atgaatattg
    35641 attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc aaagacgcca
    35701 atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac atcaatgaca
    35761 gcgtctctgt cagcactatc tgtgaatact gctgtcgctc ccgtctcttg tgccaggcgt
    35821 tcagcggcat ctttcgactc cgcataggtg aatcgtacat tggccccatc ggtgacgaaa
    35881 cgacgtacga tagcggcacc gattccacgg ctaccaccga ggatgagaac tgtcttacct
    35941 gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca aaactgtttt
    36001 aactttctgt caacaagttt tgtaatgatc actaaaaaac aatatcgcgt tccaggtcgt
    36061 cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt tcaccaaaaa
    36121 ggtttcgatg ctgtcagtgt tgctgaagtt actgattatc ttggtattaa ccccccgagc
    36181 ctctacgcgg cttttggcaa taaggctggg ttatttagcc gtgtactcaa tgaatatgtc
    36241 ggcacggaag ctattccgct tgccgatatt cttcgtgatg atcgtccggt aggggagtgc
    36301 ctggctgagg tattaaaaga agcggcgcgc agatatagcc aaaacggcgg ctgcgctggc
    36361 tgtatggttc ttgaaggcat tcatagtcat gatccacaag cgcgtgatat tgccgttcaa
    36421 tattatcacg ccgcagaaac ggccatttat gactatatcg ccaggcggca tccacaaagc
    36481 gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc gaaggcacgg
    36541 gaggggcact ccatcgaaca actctgtgca acagctgcac tggcggggga agcgataaaa
    36601 actattctca aggagtgatg cgggccttga cccgaaaggc gggaacgcgc ctgccgataa
    36661 gttgtgataa gacaataatt cacgcgttaa ggctagcgga attgatcatc ttttcgtata
    36721 acgatagaaa tgaaacgttg ttttaattaa ggagtggaaa agatgatcat cggaaatatt
    36781 cataatcttc agccgtggct accccaggag ttacgccagg cgattgagca tatcaaagca
    36841 cacgttacgg cagaaacgcc aaagggcaag cacgatatcg aaggtaatcg tctgttttat
    36901 cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta ccatgctcgc
    36961 tatctcgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag cacacaaccg
    37021 gcaggtacgc cggataccga ctggttagct gataaagaca tcgcattttt gccggaaggc
    37081 gttgatgaga aaacagttat ccttaatgaa ggtgattttg ttgtgtttta tccgggggaa
    37141 gtgcataaac cgctgtgcgc agtgggtgca ccagcccagg ttcgcaaagc agtagtgaag
    37201 atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc gagtttgtga
    37261 aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga cgttcttatt
    37321 aactcaagga gttcgtcatg agcaaaatat cagatttgaa ttattctcaa cacattacat
    37381 tagccgacaa ttttaaacaa aaaagtgaag ttttaaatac ctggcgtgtt ggaatgaata
    37441 attttgcccg taatgccgag gggcaggata acacaagaaa tatccttgat cctaagacat
    37501 ttttggaatt tttggtaaaa atatttactc taggttatgt ggatttt
//
LOCUS       DAZUGK010000022        36004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZUGK010000022 DAZUGK010000000
VERSION     DAZUGK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.022"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            21..722
                     /locus_tag="ACHY5W_000994"
     CDS             21..722
                     /locus_tag="ACHY5W_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4445931.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            747..3347
                     /locus_tag="ACHY5W_000995"
     CDS             747..3347
                     /locus_tag="ACHY5W_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS4445932.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGISWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTITVRWGEAPDQICHISYELTEQQINS
                     AITRMDAICR"
     gene            3338..4405
                     /locus_tag="ACHY5W_000996"
     CDS             3338..4405
                     /locus_tag="ACHY5W_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445933.1"
                     /translation="MQIIFGEKCVSLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYIG
                     VNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVYL
                     GDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASGN
                     SGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSLG
                     SCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSLT
                     ATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVST
                     TGVPPETGRFEATATVRINFD"
     gene            4417..4959
                     /locus_tag="ACHY5W_000997"
     CDS             4417..4959
                     /locus_tag="ACHY5W_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445934.1"
                     /translation="MKKKTIFQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4967..5482
                     /locus_tag="ACHY5W_000998"
     CDS             4967..5482
                     /locus_tag="ACHY5W_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4445935.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGAFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5448..6185
                     /locus_tag="ACHY5W_000999"
     CDS             5448..6185
                     /locus_tag="ACHY5W_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS4445936.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6296..7306
                     /gene="pyrD"
                     /locus_tag="ACHY5W_001000"
     CDS             6296..7306
                     /gene="pyrD"
                     /locus_tag="ACHY5W_001000"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFS4445937.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPRLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7480..8022
                     /gene="zapC"
                     /locus_tag="ACHY5W_001001"
     CDS             7480..8022
                     /gene="zapC"
                     /locus_tag="ACHY5W_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFS4445938.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDNFSLEQAV"
     gene            complement(8019..9128)
                     /gene="ycbX"
                     /locus_tag="ACHY5W_001002"
     CDS             complement(8019..9128)
                     /gene="ycbX"
                     /locus_tag="ACHY5W_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFS4445939.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9372..11480
                     /gene="rlmKL"
                     /locus_tag="ACHY5W_001003"
     CDS             9372..11480
                     /gene="rlmKL"
                     /locus_tag="ACHY5W_001003"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFS4445940.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLVWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11492..13399
                     /locus_tag="ACHY5W_001004"
     CDS             11492..13399
                     /locus_tag="ACHY5W_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4445941.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVYYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13529..14782
                     /gene="pqiA"
                     /locus_tag="ACHY5W_001005"
     CDS             13529..14782
                     /gene="pqiA"
                     /locus_tag="ACHY5W_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFS4445942.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14787..16427
                     /gene="pqiB"
                     /locus_tag="ACHY5W_001006"
     CDS             14787..16427
                     /gene="pqiB"
                     /locus_tag="ACHY5W_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFS4445943.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILSSQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16424..16987
                     /gene="pqiC"
                     /locus_tag="ACHY5W_001007"
     CDS             16424..16987
                     /gene="pqiC"
                     /locus_tag="ACHY5W_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFS4445944.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17243..17410
                     /gene="rmf"
                     /locus_tag="ACHY5W_001008"
     CDS             17243..17410
                     /gene="rmf"
                     /locus_tag="ACHY5W_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFS4445945.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17480..17998)
                     /gene="fabA"
                     /locus_tag="ACHY5W_001009"
     CDS             complement(17480..17998)
                     /gene="fabA"
                     /locus_tag="ACHY5W_001009"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFS4445946.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18067..19827)
                     /locus_tag="ACHY5W_001010"
     CDS             complement(18067..19827)
                     /locus_tag="ACHY5W_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4445947.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAV
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20013..20465
                     /gene="matP"
                     /locus_tag="ACHY5W_001011"
     CDS             20013..20465
                     /gene="matP"
                     /locus_tag="ACHY5W_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFS4445948.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20540..21595)
                     /gene="ompA"
                     /locus_tag="ACHY5W_001012"
     CDS             complement(20540..21595)
                     /gene="ompA"
                     /locus_tag="ACHY5W_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFS4445949.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKAHNNVTGESEKNHDTGVSPVFAGGVEWAITPE
                     IATRLEYQWTNNIGDAHTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK
                     HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQ
                     GLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRR
                     VEIEVKGIKDVVTQPQA"
     gene            complement(21952..22461)
                     /gene="sulA"
                     /locus_tag="ACHY5W_001013"
     CDS             complement(21952..22461)
                     /gene="sulA"
                     /locus_tag="ACHY5W_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFS4445950.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22680..23309
                     /gene="sxy"
                     /locus_tag="ACHY5W_001014"
     CDS             22680..23309
                     /gene="sxy"
                     /locus_tag="ACHY5W_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFS4445951.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23272..25434)
                     /gene="yccS"
                     /locus_tag="ACHY5W_001015"
     CDS             complement(23272..25434)
                     /gene="yccS"
                     /locus_tag="ACHY5W_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFS4445952.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25444..25890)
                     /locus_tag="ACHY5W_001016"
     CDS             complement(25444..25890)
                     /locus_tag="ACHY5W_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4445953.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26013..28067
                     /gene="helD"
                     /locus_tag="ACHY5W_001017"
     CDS             26013..28067
                     /gene="helD"
                     /locus_tag="ACHY5W_001017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFS4445954.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28099..28557)
                     /gene="mgsA"
                     /locus_tag="ACHY5W_001018"
     CDS             complement(28099..28557)
                     /gene="mgsA"
                     /locus_tag="ACHY5W_001018"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4445955.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28653..29315)
                     /locus_tag="ACHY5W_001019"
     CDS             complement(28653..29315)
                     /locus_tag="ACHY5W_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFS4445956.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29488..29901
                     /locus_tag="ACHY5W_001020"
     CDS             29488..29901
                     /locus_tag="ACHY5W_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4445957.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29946..30263)
                     /gene="hspQ"
                     /locus_tag="ACHY5W_001021"
     CDS             complement(29946..30263)
                     /gene="hspQ"
                     /locus_tag="ACHY5W_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFS4445958.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30321..31511)
                     /gene="rlmI"
                     /locus_tag="ACHY5W_001022"
     CDS             complement(30321..31511)
                     /gene="rlmI"
                     /locus_tag="ACHY5W_001022"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFS4445959.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAASALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31606..31884
                     /gene="yccX"
                     /locus_tag="ACHY5W_001023"
     CDS             31606..31884
                     /gene="yccX"
                     /locus_tag="ACHY5W_001023"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4445960.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLSGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31881..32210)
                     /gene="tusE"
                     /locus_tag="ACHY5W_001024"
     CDS             complement(31881..32210)
                     /gene="tusE"
                     /locus_tag="ACHY5W_001024"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFS4445961.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32301..32960)
                     /gene="yccA"
                     /locus_tag="ACHY5W_001025"
     CDS             complement(32301..32960)
                     /gene="yccA"
                     /locus_tag="ACHY5W_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFS4445962.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33368..34387)
                     /locus_tag="ACHY5W_001026"
     CDS             complement(33368..34387)
                     /locus_tag="ACHY5W_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4445963.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34365..34607)
                     /locus_tag="ACHY5W_001027"
     CDS             complement(34365..34607)
                     /locus_tag="ACHY5W_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS4445964.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(34675..>36004)
                     /locus_tag="ACHY5W_001028"
     CDS             complement(34675..>36004)
                     /locus_tag="ACHY5W_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS4445965.1"
                     /translation="DNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDK
                     ALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANIS
                     QPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQSETEQNDQKAEQNSPNA
                     LQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATCQETFDEENQAEAQ
                     EEMEGSGGDHYHTTDNESGETANPLIKVNGHREITSTSRLWHHLMIDLETMGKNPDAP
                     IISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILTDEIP
                     LDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDRDVRT
                     MVALGLMMDFDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
BASE COUNT         9174 a         8622 c         9289 g         8919 t
ORIGIN      
        1 ttttttaaga gtgactattt atgaaaactt gcataacaaa gggaattgtg accgtaagtt
       61 taacggcaat attactcagc tgctcatctg catgggcggc aggtaaaggc gggattggac
      121 ttgcagcaac acgtcttgtt tattcagaag gtgaagagca aatttcactg ggtgtacgta
      181 acaccagtcc ggatgttcct tatctgattc agtcatgggt gatgacccca gataataaaa
      241 aatcagcaga ctttattatt acaccaccgc tatttgtgct gaatccggct aatgagaatc
      301 tgttacgcat tatgtacatt ggtgcgccgt tggcgaaaga cagagaaact cttttcttca
      361 ctagcgtacg ggcagtccct tcaacaacga agcgggaaga gggaaatacc ctgaagattg
      421 ccacacaaag cgttatcaaa cttttctggc gaccaaaagg tttagcgtat cccttaggcg
      481 aggctccggc gaaactgcgt tgcacttcgt cagctgacat ggttacggtc agtaacccaa
      541 caccttattt cattaccctg acagacctga aaataggtgg aaaggtagtt aaaaatcaaa
      601 tgatttcccc ctttgataaa taccaattct ctctgccaaa gggtgccaaa aatagcagcg
      661 taacgtatcg aaccatcaat gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt
      721 aattcgtctt cagttaagag agcgagatgt atagaactca ccgacaacac agcctgttaa
      781 gctctggtgg cgtgccatcg tttattggtg ggctggtggt gtttgtgtcg gcagcgttca
      841 atgcacaagc tgaaacctgg ttcgatccag cctttttcaa agatgatccc tcaatggtgg
      901 ccgatttgtc tcgtttcgaa aaaggacaaa aaataacgcc aggggtttat cgtgtcgata
      961 ttgttctgaa tcagacaatt gtagatacgc gcaacgtcaa ttttgttgag ataacgccag
     1021 agaaggggat tgccgcctgt ttgacgactg aaagcctgga tgcaatgggc gtgaatactg
     1081 atgcatttcc ggcttttaaa caactggaca aacaagcgtg tgcgccattg gcggagatta
     1141 ttccggatgc cagcgtaact tttaatgtga ataaactccg tctggaaatt tcagtaccgc
     1201 aaattgccat caaaagtaat gctcgtggtt atgtcccccc tgaacgttgg gatgaaggga
     1261 tcaacgcgct attactggga tattcattta gcggggctaa cagtattcat agcagcgcag
     1321 acagtgattc tggcgacagt tattttctga atttaaacag tggcgttaat ttaggcccat
     1381 ggagattgcg caacaattca acatggagtc gtagtagtgg ccaaaccgca gaatggaaga
     1441 atctcagcag ctatttgcaa cgggcggtta ttcctctgaa aggcgaactg accgtaggtg
     1501 atgattatac tgcaggcgat tttttcgata gtgtcagctt tcgtggtgtg cagctggcgt
     1561 cagatgacaa catgctgcca gacagcctga aagggtttgc gcctgtggtg cgtggtatcg
     1621 ccaaaagcaa tgcccagata acgattaagc aaaatggtta caccatttac caaacttatg
     1681 tatcgcctgg tgcttttgaa attagtgatc tctattccac gtcgtcgagc ggtgatttgt
     1741 tagttgaaat caaagaagcg gacggcagcg tcaatagcta cagcgtaccc ttttccagcg
     1801 tgccattact ccagcgtcag gggcgaatca aatacgcggt gacactggcg aaatacagaa
     1861 ccaatagtaa tgaacagcag gagagcaaat ttgcccaggc cacgttgcag tggggcggac
     1921 cgtggggaac gacctggtat ggtggtggac aatatgctga atattaccgt gccgccatgt
     1981 ttggtctggg atttaacctc ggcgatttcg gagcaatttc gttcgatgtg acccaggcga
     2041 agagtacgct ggcagaccaa agcgaacata aaggtcagtc atatcgtttt ctgtatgcca
     2101 aaacgctcaa ccaattgggt actaactttc aattgatggg ctatcgctat tcgacgtcgg
     2161 gtttctacac cctttccgac accatgtata aacatatgga tggctacgaa tttaatgacg
     2221 gtgatgatga agatacgccg atgtggtcgc gttattacaa tttgttttac accaaacgtg
     2281 gcaaactgca ggtcaatatc tcccagcaat taggcgagta cggttcgttt tatttaagtg
     2341 gtagccagca aacttactgg cataccgatc aacaggatcg gctattacag tttggctaca
     2401 acacgcaaat taaagatctc tcgctaggga tctcctggaa ttacagtaag tcccgtggtc
     2461 aacctgatgc tgatcaggtg tttgcactta atttttccct gccactcaat ctgttgctcc
     2521 ccagaagtaa tgatagctat accaggaaaa aaaattacgc ctggatgacc tctaacacca
     2581 gtatcgataa cgaagggcac actacacaaa acctgggttt aacggagaca ctactcgatg
     2641 acggtaacct gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg
     2701 gtagcgccag catggattac aaaggggcgt ttgcggatgc ccgagtgggc tacaactaca
     2761 gcgataacgg cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt
     2821 cacagggcat taccctgggg caatcgctgg gggaaactaa cgttctgatt gcagcaccag
     2881 gcgcagagaa tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgtggatata
     2941 ccgttgttcc ttatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcgtcgt
     3001 taaaacgtaa cgtggatctt gaaaatgcag tagtcaatgt ggttcccacc aaaggggcgt
     3061 tggttctggc ggagttcaat gcccatgcgg gtgcaagggt attaatgaaa acatcaaagc
     3121 agggtatacc gctgcgtttt ggcgcgatag cgacgctgga cggcgtacag gctaatagcg
     3181 gcataattga tgatgatggc tcgctctata tggcgggttt accggcgaag ggaacaataa
     3241 ccgtgcgctg gggcgaagca cccgatcaaa tttgtcatat cagttacgag cttaccgaac
     3301 aacaaattaa ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa
     3361 atgcgtgtca ttacgactat tttttgccgc cgtcttaatg ctatggtgcg ctcaaaccgc
     3421 tgcttatagc gggcagtgtc ataccactca ggggaatccc tatattggcg tcaattttgg
     3481 cgttaaaacc ctggaggaag aagaaaatac tgcaggggta gttaaagaca aattttatca
     3541 gtggaacgaa tcgaatgatt attatgtttc ctgtgattgc gataaagaca atgtcagaaa
     3601 tggtcgatgg gcattcgccg cagattcacc gttagtctat ttaggcgaca actggtacaa
     3661 aattaatgac tatcttgccg ccaaggtttt attgcaggtt aaaggcagtt ctcctacagc
     3721 ggttcctttc gaaaacgtgg gcacaggggg ggatacccga tggcatattt gcgaccctgg
     3781 cggtcaacgt ttaggtggtc agggggcaag cggtaatagc ggtagctttt ccctgaaaat
     3841 attgcagccg ttcgttggct cggtcgtcat tcctcctatg gcgctggcgc gattatttga
     3901 atgctacaac atacccgcag gtgattcctg cacgactaca ggtacaccgg ttttagtgta
     3961 ttacctgtct ggtacgatca attcacttgg ctcatgttcc gtcaatgccg gagaaacaat
     4021 cgaggtcgat ctgggcgacg tatttgcggc taactttcga gttgtagggc ataagcctct
     4081 tggggccaga acggcagaac ttgcaattcc agtcaggtgt aacacgggaa acgcggggtt
     4141 agttaacgtc aacctgagtc tgacggcaac ctcagacccc agctatcccc aggcgattaa
     4201 gacgtcacgt cctggcgtgg gcgtggtggt gaccgatagc cagaacaaca ttatttctcc
     4261 agcgggtgga acactaccgc tctctattcc tgatgatgca gacagtatcg cgcgaataaa
     4321 tgtctatcca gtcagcacga caggtgtacc accagaaacc gggcgatttg aagccacggc
     4381 aacggtgaga ataaattttg attaacgcgt gaacctatga agaaaaaaac gatatttcag
     4441 tgcgttatct tgttctttag ccttcttaat atccatgtcg ggatggctgg gcctgaacaa
     4501 gtcagtatgc atatttatgg gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt
     4561 gcattacaaa atattcatat tggtgatttt aatatcagtg attttcaggc cgcgaatacc
     4621 gtaagcactg ctgctgattt gaatatcgat atcaccggtt gtgcagctgg tattactggt
     4681 gcggacgtcc tttttagcgg cgaggctgac acccttgcgc cgacactgct caaattaact
     4741 gacacaggcg gaagcggtgg tatggcaacg gggattgccg tgcaaattct tgatgcgcaa
     4801 agtcagcaag aaatcccgct caatcaggtc cagcctctta cgcccttaaa agccggggat
     4861 aacacactca aatatcaact tcgttataag tccacaaagg cgggagcaac gggcggtaat
     4921 gcgacggcgg ttctctattt tgatctggtt taccagtgaa ggagtgatgt tgaaacggat
     4981 aatctggatt ctgttcttac tgggattaac ggggggttgt gagctatttg cccatgatgg
     5041 cacggtcaac attagcggag cgtttcgccg taatacatgc gtgctggcac aggatagcaa
     5101 gcaaatcaat gtgcagttag gcgatgtctc actgacacgt ttttctcatg ggaattatgg
     5161 cccggagaaa tctttcatca ttaatttgca ggattgcgga acagatgtca gcacggttga
     5221 cgtcaccttt tcaggaaccc ccgacggagt gcagagcgaa atgttgtcca ttgaaagtgg
     5281 tacggatgca gccagcgggc tggcaattgc gattctggat gatgcgaaga tattgattcc
     5341 gcttaaccag gcgagtaaag actacagcct acatagtggt aaggtgccat taacttttta
     5401 tgcgcaattg cgacctgtca atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac
     5461 atttgtactt cattatgact aatacgtgga acagattggc gctcttgatt ttcgccgttt
     5521 tatcgctgct ggtggcgggt gaattgcagg caggagtggt ggtcggcgga acgcgattta
     5581 tctttccggc agacagagaa tcgatatcta ttttactgac taataccagt caggaatcct
     5641 ggcttattaa cagtaaaatc aaccgcccaa cgcgttgggc cgggggtgaa gcgtcgacag
     5701 tgccagcacc attactggcc gctccgccac ttattctcct gaagcccggt acgacaggca
     5761 cgttgcgctt gctgagaacg gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg
     5821 agttaagcat tgccagcgtg ccatccggca aagttgaaaa tcagagcgta aaagtggcga
     5881 tgcgctcggt atttaaactg ttctggcgac ccgaaggttt gccgggcgac ccgctggaag
     5941 cttatcaaca attacgctgg acacggaatt cgcagggtgt acaactcact aacccaacgc
     6001 cttattacat taacctgatt caggtgagcg taaatggtaa agcgttaagt aatgtgggag
     6061 ttgtgccgcc taaaagccag cgtcagacaa gctggtgtca ggcaatagca ccgtgtcatg
     6121 ttgcctggcg ggcgataaat gattacggcg ggttgagtgc aaagaaggag caaaatctgc
     6181 cctgaaacag gttcggaaaa cgtttgcgtt ttttttgccg caggtcaatt cccttttggt
     6241 ccgaactcgc acataatacg cccccggttt gcacaccggg aatccaggag agttcatgta
     6301 ctaccccttc gttcgtaaag cccttttcca gctcgatcca gagcgcgctc atgagtttac
     6361 ttttcagcaa ttacgccgca ttaccggaac accgtttgaa gcactggtgc ggcagaaagt
     6421 gcctgcaaaa cctgttaact gcatgggcct gacgtttaaa aatccgcttg gtctggcagc
     6481 cggtcttgac aaagacgggg agtgcattga tgcgttaggc gcgatgggat ttggatcgat
     6541 cgagatcggt accgtcacgc cacgtccaca gccaggtaat gacaagccgc gtctctttcg
     6601 tctggtagat gccgaaggtt tgatcaaccg tatgggcttt aataatcttg gcgttgataa
     6661 cctcgtagag aacgtaaaaa aagcccatta tgacggcgtc ctgggtatta acatcggcaa
     6721 aaataaagat acgccagtag agcagggcaa agatgactat ctgatttgta tggaaaaaat
     6781 ctatgcctat gcgggatata tcgccatcaa tatttcatcg ccaaataccc caagattacg
     6841 cacactgcaa tatggtgaag cgctggatga tctcttaact gcgattaaaa ataaacaaaa
     6901 tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca gtgaagatcg cgccggatct
     6961 ttctgaagaa gaattgatcc aggttgccga tagtttagtt cgccataata ttgatggcgt
     7021 tattgcaacc aataccacac tcgatcgttc tctggttcag ggaatgaaaa attgcgatca
     7081 aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc accgaaatta ttcgccgctt
     7141 gtcactggaa ttaaacggtc gcttaccgat catcggtgtt ggcggcatcg actcggttat
     7201 cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg caaatttatt ctggttttat
     7261 ttttaaaggt ccgccgctga ttaaagaaat cgttacccat atctaattat ttcttcgaat
     7321 tcctctttat aagataatca gggctttatt ttcagccctg gttgttttat attcatcact
     7381 gttgcttatt tagacatttt gtacttttat tgttgaggtt attaagcgaa gcgacaatgg
     7441 attgtgttgc tgcggtttta taaggttggg gagaggcaga tgcgaattaa accagatgat
     7501 aactggcgtt ggtattacga tgaagagcac gatcgtatga tgctcgattt agccaatggt
     7561 atgctatttc gctcacgttt tgcgcgcaag atgttgactc cagatgcctt ttctcccgca
     7621 ggcttttgcg ttgatgatgc cgcgctctat ttctcttttg aagaaaagtg ccgcgatttt
     7681 aatttatcta aagaccaaaa agccgaactg gtgctgaatg cactggtagc aattcgctat
     7741 ctcaaaccgc aaatgccgaa aagctggcat tttgtttccc atggtgaaat gtgggtgccc
     7801 atgccgggcg atgcggcctg cgtttggttg agcgacacta acgagcaagt gaatttactg
     7861 gtcgttgaat ctggcgagaa tgccgcgcta tgcctgctgg cacaaccctg cgttgttatt
     7921 gcgggtcgcg cgatgcagtt gggcgatgcc attaaaatca tgaacgacag gctgaaaccg
     7981 caggttaatg ttgacaactt cagcctcgaa caggcagtct aacgcgccaa cttaagtgca
     8041 gtcttcggta cacagctaca gcaaagaatg gtgccatcat cgcccattgc tgattttttc
     8101 agcggcgtga cttcgccttc taaaagctga acacggcaac ttccacaaat gcccgcgcgg
     8161 caagaataag ggatacgaat tccctgattt tctaattgct ccagcaacac ttgttggtta
     8221 tttccacgaa atgcctgtcc ctgccagtca atatctacat ttgcgtccgg ttgttgcgtg
     8281 atgttggcgg tatcatcagc ggcagctgcg ccgtaaattt ttgccggagc cgttgccaga
     8341 atttccacct catcgccaac gcgaatcacg ccgctattac gggcaattaa attctgacca
     8401 aaatcgacat cgccgttatc ctgggcagtg cggaaagatt gcaatgtttt taatggttcg
     8461 cctgccggat gtttttgccc tttttctggg ctgacggtgg tgaaaatgca gcggctacaa
     8521 ggtttaacca catcaaacac cacatcacca atacgaatca ctttccagct atcttcttcc
     8581 caggctgacg cgccggaaac caccagattg gggcggaatt gctccatttt tacactggcc
     8641 ggacaacgtt gttggagatc acgtaacgag gcctcgttag caagaaggta aggatagcca
     8701 tcagcaaatg acaggggtac agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc
     8761 acccagcgta attgcacttc gcgggagaaa aatccactta gccatttgtt gatcgcgtct
     8821 ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg
     8881 aaatcagcaa aacgaacata tgcgctactg ccatctggtg cggtgagatg caagccatca
     8941 tgcacaggtg aaggagtaaa ccgtaccatc tggggaaact ggcgagcggt aataaacgta
     9001 ccatcaggtt ccgtgatcat aaagatgcga tcgaaggcca gaccactgac atctgccagg
     9061 gtatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat gaataaaaag ccgggttaat
     9121 gtcgccacgg tgtggtcctc aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt
     9181 attagctata atgcgcaaca attttcttag cgcctgtccc attaggctat tttatttgtc
     9241 agtttggccc tgggttgtgc tcgaaatcct cacgtacttg tgtacgctcc ggtttctccg
     9301 cgctgtccat gtccagactg actgcaacaa ttacgcctac tgcggtaggc tcttagagta
     9361 aaagtgacga tatgaattct ctgtttgcca gtacggcccg tgggctggaa gagctgttaa
     9421 aaactgaact ggaaaacctg ggggccgttg aatgccaggt ggttcagggt ggggtccatt
     9481 tcaagggcga cacacggctt gtttaccaga gcctgatgtg gagccgcctg gcctcgcgta
     9541 ttatgttgcc gctgggcgag tgtaaggttt acagcgattt agacctctat ctcggtgttc
     9601 aggcgatcaa ctggacagag atgtttaatc ctggcgcgac cttcgctgtc cacttcagtg
     9661 gtttgaatga caccatccgc aacagtcagt acggcgcgat gaaagtgaaa gacgcgatcg
     9721 tcgatgcttt cacgcggaaa aatctgccgc gtccaaacgt tgatcgcgat gcgccagata
     9781 tccgcgttaa cgtctggctg cataaagaaa ccgccagtat cgcccttgat ctcagtggtg
     9841 atggtttaca tctgcgtggc tatcgcgatc gtgctggtat tgcgccgatc aaagaaaccc
     9901 tggcagccgc tattgtgatg cgatccggct ggcagccagg aacaccgctg ctcgatccga
     9961 tgtgtggttc cggtacgttg ctgattgaag cggcgatgct ggcgaccgat cgcgcaccag
    10021 gcttgcaccg tgggcgttgg ggctttagcg gctgggcgca gcatgatgaa gctatctggc
    10081 aggaagtgaa agcggaagcg caaactcgcg cccgtaaagg cctggctgag tatagctctc
    10141 atttttacgg ttcggacagc gacgcacggg tgattcaacg tgcacgcact aacgcccgtc
    10201 ttgcggggat tggtgaactg atcacctttg aggtgaaaga tgtcgcgcaa ctgaccaatc
    10261 cgctgccgaa agggccgtac ggcacagtgt tgagcaaccc gccatacggg gaacgtctgg
    10321 acagcgaacc ggcgctgatt gcgctgcata gcctgctggg tcggatcatg aaaaaccagt
    10381 ttggtggctg gaatctctca ttgtttagtg cctcgccgga tctgctaagc tgcttgcagc
    10441 tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct ggactgcgta cagaaaaatt
    10501 accacgttgc cgaaagcact ccagacagca aaccggcgat ggtagcggaa gactacgcca
    10561 accgcctgcg taagaacctc aaaaaattcg agaagtgggc tcgccaggaa ggaattgaat
    10621 gttaccgcct gtatgacgcc gatctgccgg aatataacgt tgctgttgac cgttatgccg
    10681 actgggtggt ggtgcaggag tatgcaccgc caaaaactat tgatgcgcac aaagcgcgtc
    10741 agcgtctgtt cgatattatc gctgcaacca tttcggtact ggggattgcg ccaaacaaac
    10801 tggtgctgaa aacccgtgaa cgccagaagg gcaaaaacca gtaccagaaa ttgggcgaga
    10861 agggcgagtt tcttgaagtt accgaatata acgctcactt gtgggtgaac ctgacggatt
    10921 atctcgatac tggtctgttc ctcgatcacc gtatcgcccg ccgtatgctc ggtcagatga
    10981 gcaaaggcaa agatttcctc aacctgtttt cttataccgg cagcgccacc gtgcatgcgg
    11041 gattaggcgg tgcacgcagc accaccaccg tggatatgtc gcgtacttat ctggtatggg
    11101 cagaacgcaa cctgcgtctg aatggcctga ccgggcgtgc gcatcgcctg attcaggccg
    11161 attgcctggc gtggctgcgt gaggcaaatg aacagttcga tctgatcttt atcgatccgc
    11221 caaccttctc taactcaaaa cgaatggaag atgcgtttga tgttcagcgc gatcatctgg
    11281 cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg gacgatcatg ttctcgaaca
    11341 acaaacgtgg cttccgtatg gatctcgacg gcctggcaaa actgggactg aaagcacaag
    11401 aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa ccgccagatc cacaactgct
    11461 ggctgattac cgcagcctga aaggaatagt aatgtcatta atcagtatgc atggcgcatg
    11521 gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg aagataacga
    11581 acgtgtttgt ctggtaggcc gtaacggcgc gggcaaatca acgttaatga agatcctcaa
    11641 ccgtgaacag gggctggatg acgggcgcat tatttacgag caagatttga ttgtggctcg
    11701 tctgcaacag gatccgccgc gtaacgttga gggcagcgtt tatgatttcg ttgccgaagg
    11761 cattgaagaa caagcggaat acctgaaacg ctatcacgat atttcgcgcc tggtgatgaa
    11821 cgacccgagc gagaaaaatc tcaacgaact ggcgaaagta caggaacagc tggatcatca
    11881 taatctgtgg caactggaaa accgcatcaa cgaagtgctg gcgcaactgg ggttagatcc
    11941 taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat taggacgcgc
    12001 gctggtgagc aatccgcgcg tattgctgct ggacgaaccg actaaccacc tggatatcga
    12061 aaccatcgac tggctggaag ggttcctcaa aaccttcaat gggacgatta ttttcatctc
    12121 ccacgaccgc tcgtttatcc gcaatatggc gacgcgtatt gttgatctcg atcgcggcaa
    12181 gctggtgacc tatccaggga attacgacca gtacctgctg gaaaaagaag aagccctgcg
    12241 cgtggaagaa ctacaaaatg ccgagttcga tcgcaaactg gcgcaggaag aggtgtggat
    12301 ccgccagggg atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg ccctgaaagc
    12361 aatgcgtcgc gagcgtggtg aacgtcgcga agtgatgggt accgcaaaga tgcaggtgga
    12421 agaggccagc cgctccggta agatcgtttt cgaaatggaa gacgtttact accaggttga
    12481 cggtaagcaa ctggtgaaag atttttctgc ccaggttcta cgtggcgaca aaattgccct
    12541 gattgggccg aatggctgcg gcaaaaccac gctgctgaaa ctgatgctcg gtcagcttca
    12601 agcggacagc ggacgtattc acgttggcac caaactggaa gtggcttatt tcgatcagca
    12661 ccgtgcggaa ctggatcccg ataaaacggt gatggataac cttgccgaag gtaagcaaga
    12721 ggtgatggtt aacggcaagc cacgccacgt tttgggctat ttgcaggact tcctgttcca
    12781 tccgaaacgg gcgatgacgc cggtacgtgc gctttctggc ggtgagcgga accgcttgct
    12841 gctggcgcgt ttgttcctca aaccaagcaa cttattgatt cttgacgaac cgaccaacga
    12901 tcttgatgtc gaaacgctgg aactgctgga agaactgatc gacagctacc agggcacggt
    12961 attgctggtt agccacgatc gtcagtttgt tgataacacc gttacagaat gctggatctt
    13021 cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat catgacgccc gtggtcagca
    13081 agaacagtat gtggcgctta aacaacctgc ggtgaaaaaa aacgaagaag ccgctgcggc
    13141 aaaagcagaa actgtaaaac gcagcagtag caaactaagc tataaattgc agcgcgaact
    13201 ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc tacagacgca
    13261 ggtggcggat gcttccttct tcagtcagcc gcatgagcag acacaaaaag tgctcgccga
    13321 tatggctgct gcagagcagg agctggagca agcctttgaa cgctgggagt atcttgaagc
    13381 gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa cagagacagc tgacaacggt
    13441 aaaattgcct gatgaactgg gtttatcagg catgaaaacc aacactttgt tagtcatctg
    13501 taaaatagcg catcattaag gagtaccaat gtgcgaacat catcatgccg cgaagcacat
    13561 cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg gtcagaaagc
    13621 ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg gatgcccccc ggcagcgtcc
    13681 taccgcctat gcgttggctg cactgttcat gctgttgctg tccaacttgt ttccttttgt
    13741 gaatatgaac gttgcaggag ttaccagtga aataacatta ctggaaattc ccggcgtgct
    13801 tttttctgag gactacgcca gcctcggcac ctttttcctg ttgtttgtgc aactggttcc
    13861 cgcgttttgt ctgataacca ttctgttact ggtgaatcgc gcggaattac cggtccgttt
    13921 aaaagagcaa ctggcacggg tgctttttca actcaaaacc tggggaatgg cggagatttt
    13981 cctcgcgggt gtgctggtca gtttcgttaa actgatggct tacggcagca ttggcgtagg
    14041 cagcagcttt ctcccctggt gtttattttg tgtcctgcaa ctgcgcgctt ttcagtgcgt
    14101 tgatcgtcgc tggttatggg acgatatcgc cccgatgcca gaactgcgcc agccgctaaa
    14161 accaggcgtc acggggatac gtcaggggct gcgttcgtgc tcctgttgta cggcaatcct
    14221 tcctgctgat gaacccgtgt gcccgcgctg tggtactaaa gggtacgttc gacgtagaaa
    14281 cagcctgcaa tggacactcg cgctgcttgt tacgtccatc atgctgtatc ttccggctaa
    14341 tattttgccc atcatggtga cggatttatt aggctcgaag atgccatcga cgattctcgc
    14401 tggggtcatc ctgttatgga gcgaaggctc ttatcccgtc gctgcggtga tctttctggc
    14461 cagtattatg gtgccaacgt taaagatgat cgccatcgcg tggctgtgtt gggatgccaa
    14521 agggcatggc aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg ttgagtttgt
    14581 aggccgctgg tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc tggtgcgtat
    14641 gggaggttta atgagtattt atccggcaat gggtgcatta atgtttgctt tagtcgtcat
    14701 aatgacaatg ttttctgcta tgacgtttga cccgcgtttg tcgtgggatc gtcaacctga
    14761 atcagagcat gaggagtcct gacagtatgg aatctaataa tggggaagcc aaaatccaga
    14821 aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt cacggcgctc attggggcct
    14881 gggttctttt ttatcattac agccatcagg gaccggaagt gaccctgatc accgcgaatg
    14941 cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg tagcgttgac gtcggcgttg
    15001 ttgaaagcgc cacactcgct gatgatttga cacacgttga aatcaaagcg cggctgaatt
    15061 ccggtatgga aaaattgctg cataaagata ccgtcttttg ggtggtgaaa ccgcagattg
    15121 gtcgagaagg gattagcggt ctgggaaccc tgctgtctgg ggtttatatc gaactgcagc
    15181 caggcgcgaa aggcagcaaa atggataaat acgatttgct ggactcgcca ccgttggccc
    15241 cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa aaaagccggg cagctctcgc
    15301 caggagatcc ggtgctgttc cgtggctatc gggtaggttc ggttgaaacc agcaccttcg
    15361 atacgcaaaa acgcaatatc agttatcaac tgttcatcaa tgcaccttat gaccgactgg
    15421 tgaccagcaa tgttcgcttc tggaaagata gtggcattgc ggttgatctg acgtcagcgg
    15481 gaatgcgtgt ggagatgggc tcattgacaa cgctgctgag tggcggtgtc agctttgatg
    15541 tgccggaagg tctggattta gggcagccag tggcaccgaa aacagctttc gttttgtatg
    15601 atgatcagaa gagcattcag gattcgttgt acaccgatca cattgattat ctgatgttct
    15661 ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt agaattccgg ggtattcgcc
    15721 tgggtaccgt aagcaaagtg ccattctttg cgccgaatat gcgtcagaca tttaacgatg
    15781 attaccgtat tccggtgctg attcgtatcg agccagagcg gctgaaaatg cagcttggcg
    15841 aaaatgcgga tgttgttgag caccttggcg aattgttgaa acgtggttta cgcggatcgc
    15901 tgaaaaccgg aaacctggtc actggcgcac tgtatgttga tctcgatttc tatccaaata
    15961 cgcctgcaat aaccggtatt cgtgaattta atggttatca gattatcccg accgttagcg
    16021 gcggcctggc gcaaatccag caacgactga tggaagcgtt ggataagatc aacaaactgc
    16081 cattgaatcc gatgattgaa caggcaacca gtacgctttc tgaaagtcag cgcacaatga
    16141 aaaacctgca aacgacgctg gatagcatga acaagatcct ctccagccag tcgatgcagc
    16201 agttgccgac ggatatgcag tcaacgttgc gtgaattgaa tcgcagcatg cagggcttcc
    16261 agcctggctc cgcagcctac aacaagatgg tggcggatat gcagcgcctt gatcaggtgt
    16321 tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa gagtaacgcg ctggtatttg
    16381 aagcgaagga caaaaaagat ccagagccga agagggcgaa acaatgaaaa agtggctagt
    16441 gacgattgca gcactgtggc tggccggatg cagctccggc gaaattaata aaaactatta
    16501 ccagttaccg gtggtgcaga gcggtacaca aagtaccgcc agccagggta atcgcctgtt
    16561 atgggtagag caggtcactg ttcctgacta tctggcgggg aatggtgtgg tttatcaaac
    16621 cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg gccagcccgt tggatcaaca
    16681 gttgcgcaac accctggttg ccaatctgag cacgcaactg cccggctggg tggttgcctc
    16741 ccagcctctg ggaagcgccc aggacacgct caatgttacc gtaacggagt ttaacggtcg
    16801 ctatgatggc aaggtcattg tcagtggtga gtggctgttg aaccaccagg gacaactgat
    16861 caaacgtccg ttccgtctgg aaggagtgca aactcaggat ggttacgatg agatggttaa
    16921 agtgctggcc agtgtctgga gtcaggaagc cgcttctatt gcacaagaga taaagcgtct
    16981 accttaatta taaagatttg taaatataac cgtctccggt atgttgcctg aggcggtttt
    17041 tttgtttcta acgtgcggaa aaagttgttc ctcttcacat tttttgtaca accgacatgc
    17101 ccgtgtagct cacaaatatg acagtggcgt gaattttgcg cattgacggc agttatgatt
    17161 cggggtattg cttaactgtg attgcacatt tagtaatcac tgttttcttt tccaccagaa
    17221 accagtatga gggaaacgag gcatgaagag acaaaaacga gatcgcctgg aacgggcaca
    17281 tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc cctatcagac
    17341 gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa gccatggcgg acagggtagt
    17401 aatggcctga ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt tcgccttttg
    17461 atactctgtc tgattataat cagaaggcag acgtatcctg gaacagaccg actttcaggt
    17521 cgctggcggt atagatcaga cgaccatcaa ccagcacttc gccatctgcc agacccataa
    17581 tcagacgacg attaacaatg cgtttaaagt gaatacggta ggtcactttt ttcgctgtcg
    17641 gcagcacctg accagtgaat ttcacttcgc caacgcccag cgcgcggcct ttaccttcgc
    17701 cgcccagcca gccgaggtag aaccctacca gctgccacat tgcgtccagg cccaggcatc
    17761 ccggcataac cggatcgcca ataaagtggc atccgaagaa ccacagatcc ggattgatat
    17821 ccagttctgc ttcaacatac cctttgtcga agttaccacc cgtttcggtc attttgacca
    17881 cacggtccat catcagcatg ttcggtgctg gcaattgcgg gcctttagcg ccaaacagtt
    17941 caccgcgacc agaggcaaga aggtcttctt ttgtatagga ttcgcgttta tctaccatgt
    18001 tctctgtaag ccttatttta ttgaagcacg caggatagct aacacgtgta cgctgaacaa
    18061 gtccgatcag ttcggaataa accagttcag ccaacgtaat ggccatggaa aacggtgacg
    18121 tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca gcgtcgtttg
    18181 gccttcgcca tcccacacca gatttaataa taacggcagt gcgtcagtca catcgtctac
    18241 tgcccagatg gtgaatttgc cttcttctac cgctttcacc agttcactgt gaagacttaa
    18301 atggcgaacg ttagcagtgg ggataatgac accttgtttc cccgttaact cacgttgctg
    18361 gcaaatagca aagaagcctt cgattttctc atttaaacca ccgaccggct gggcgcgacc
    18421 gaactgatcg actgaacctg tgatagcgat actctgattc accggcacat cggcgagggc
    18481 gcttatcaga gcgcatagtt cagccatcga ggcactatct ccatcaactt cactgtatga
    18541 ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta gttccgacat
    18601 caggaacgcc tgcatgatca tcatcccttt cgcatggata ttgccgccaa gctccgcttt
    18661 gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca acgcagctaa tgcgagaagg
    18721 ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt taatttgccc
    18781 gatgcgttcg ccttcggttt caatcaggat ttgctcctga aggatctcat cctgcatacg
    18841 ttcagcgagg aaaccttcgc gccattcacg ctgttgcagc attaagttta gctgctcgcc
    18901 ggagaaggtg tcgccatcac acagggaggc gacctcttta cactggcgga ggatccactg
    18961 cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt actgcttcgc ggataagtac
    19021 cggccaggca tccgctcccg gtgcaggtaa gtgattatgt ctggcggtaa atgtcaccca
    19081 gcgacaccac tggcttactg actccgcatc gacaatctgc agagtatctt caaattcgct
    19141 ataaatagcc tgctctgaaa gctctggctc catctcctgg aaatcagcca atgattcgcg
    19201 ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa ggcacagaga cggggagagg
    19261 gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat ttttcagccg
    19321 catccacagc agaggttgcg ccagcagtgt acgcaaagag atgatgagaa taccgccatt
    19381 tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc
    19441 aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt
    19501 atcatctgca ctcactgcgt tacgtaagcg gatggtgtgg ccggaaacct catagtgacc
    19561 gcccaccagt tggcctgcat cgctttgcag cgtacgcgcg gcatcggcaa taagattcag
    19621 atactcagac tcttccggag ccttcgccag cataaaagag gaggatgctc gcgtatgcag
    19681 caactgctcc agcgcaaatt gcagtcgcgg ttgagtatca ctgaataaag gatcgttttc
    19741 gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag gaaccaggtc
    19801 acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg tcaaagctgc
    19861 gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac tcctggcatg
    19921 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    19981 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    20041 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaatcaccc
    20101 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctggaaaatg
    20161 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    20221 tgaagcaaac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    20281 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcgcagc
    20341 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    20401 agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg ctgggtaagg
    20461 aataactgac gaaagtcatt caatttacta aaggcaaaaa aaaccccgca gcagcggggt
    20521 ttttctacca gacgagaact taagcctgcg gctgagttac aacgtctttg atacctttaa
    20581 cttcgatctc aacgcgacga tccggagcca ggcagtcgat cagtgcagca cgctgtttca
    20641 cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagatct
    20701 tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca cggcgctcgg
    20761 acagaccctg gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga acaactacgg
    20821 aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac
    20881 cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc agagtgaagt
    20941 gcttggtctg tacttccggt gccggagctg gagccggagc aactaccgga gctgcttcgc
    21001 cctgaccgaa acggtaggaa acacccaggc tcagcaggcc gttgtccgga cgagtgccga
    21061 tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag
    21121 gagtgatggc ccattcaaca ccacctgcga ataccggaga aacgccggta tcgtggtttt
    21181 tctcagattc acctgtcaca ttgttgtgag ctttggtgtc tgcacgccaa accataccac
    21241 ccagacgagt gtacacgtcc aggtcgtcag tgattgggta acccagttta gcggtcaact
    21301 gaacgccctg agctttgtat gcaccgtttt caacgctgcc tttgtacggc atacgaccta
    21361 accagtcgta acccatttca aagccaacat acgggttaac ctggtaacca ccaaaagcac
    21421 cagcgcccag ttggttttca tgggtcgggc cattgttgtc gatgaaacca gtgtcatggt
    21481 actgggacca gcccagttta gcaccagtgt accaggtgtt atctttcgga gcggcctgcg
    21541 ctacggtagc gaaaccagcc agtgccactg caatcgcgat agctgtcttt ttcatttttt
    21601 gcgcctcgtt atcatccaaa atacgccatg aatatctcca acgagataac acggttaaat
    21661 ccttcaccgg gggatctgct caatattaac tctaccgata tcttcggctt atgccgagca
    21721 cccctagcga tgtaaagtct acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg
    21781 catatgaaaa aaaagtcttg tataaggtac gtttaatctt ttttgtcagc gacaatttac
    21841 agaagaaaat cgcggaaacc gcttcagaca agcctccgca aggaaaatta gtcacgactg
    21901 aaagcattgg ctgggcgaca aaaaaagttc caggattaat cctaaattta cttaatgata
    21961 caaattagag tgaattttta gcccggaaag ttgtctggtt gcgtgagagg atgcgcttac
    22021 cggacgcata ataaacccca tagcgttacc ttcatttgcc gcatcaacaa gttcagcatg
    22081 ctcttcttca gtcaaatcat ctgccaacca accgatcacc acactgtaat tgcccgtgcg
    22141 taaagcgcga accattgact ctacggtgtg gcaaggggag agctggctaa tctgcattac
    22201 tttcgttaag ggtagcccag atgcctgaac ccattcccga ctcagttttt gttgcggtgt
    22261 taaccagagt tgccagcgcg attgctgacc gagttgctgt aacaatggca acagtagaag
    22321 ttgcgtcatc atgggctgat cttcgcgata gacaacttca ctgataagcc cggctgtagt
    22381 gttttccgta gagacacgcg caattttact tgctgcggat gagaacgacg aagaacgatg
    22441 tgcatagcct gaagtgtaca taatcaatcc agcccctgtg agttactgta tggatgtaca
    22501 gtgcatccag tgacaacaaa gatcaaccct attttcggaa agagcctcgc aaattttgtc
    22561 gttggtgacg ggaaaacata aattaatctt gccccttaag aataagttgc ctattttcgt
    22621 agttaacgga tccgttaatg tgaatcattc ttttatgtta tgattttaaa aggaatttta
    22681 tgaaaagcct ctcctataag cggatctata aatcacaaga atacctggca acgttgggca
    22741 caattgaata ccgatcattg tttggtagtt acagcctgac cgttgacgac acggtgtttg
    22801 cgatggtttc tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca cagtactgtg
    22861 taaaacatcc gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt accctcaatt
    22921 actatcgggt tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt ctgtcgaaat
    22981 attctctcga tgcagcgctg aaagagaaaa gcacgcgcaa tacccgggaa agactgaaag
    23041 atttgcccaa tatgtctttt catctggaag cgattctcgg ggaggtgggg attaaggatg
    23101 tacgggcgtt acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg cagcaaaaca
    23161 gtctggtgac agaaaagatt ctgtttatgc ttgaaggtgc cattatcggc attcatgaag
    23221 ctgcgctccc ggtggcacgc cgccaggagc ttgcagaatg ggctgactct cttacgccga
    23281 aacaggagtt tcctgcggaa cttgagtaat ctggcgttgc agacgaccaa tctcaggcag
    23341 taatgcaatc aataacccaa cttgttgtac gaccagaggt tctttgctgt ctgcccgtgg
    23401 ttcaagttgc tgcatccgct gtttcaggcc agctaatgcc tgattgacgc gttcttcatc
    23461 agcaggttga tgatgtaacg cgtcatcaac atagcaaact gcgtcatcaa gaaacgccag
    23521 aatttcagga ttagttaact gctcccggtg agcaccgagg gctgagatat agctggtaaa
    23581 cgtatggtta aggcacagca accgaaacgc ggcttcgcga atttgcgggg taacgttcgg
    23641 ttcgctggac atatttgata ccaccgacgc cagttcagca tcacggttgt gtgcatcgcg
    23701 gcgggcaata cgatacgcca gacggttatc acgcccctga tggtattgct ccagtatggc
    23761 atcgagatag cgacagttgg cctctgtggc gcgttcgagc attcgcggca gattgcgaaa
    23821 cttccagtca ggccagatgt agctcactgc cgcccacgca atggcacaac caatcagcgt
    23881 atcgattacg cgaggtaacg ctacttcaaa accttcaccc agcaagttaa aacacagtag
    23941 caccaaaagt gtgatgaaca tcgttgcatg ggcatattgc acgttacgga aggcaaaaaa
    24001 gagcacgccg gtaataacca gtagcaccag ctgcccttcc agtgacggta caaaccacag
    24061 cacaggaatg ccaatggcga tacctaccag cgtaccaata atccttaact tcaggcggtg
    24121 gcgcgtggcg ttatagtttg gctggcagac aaacaaactt gtcagcaaga tccaataccc
    24181 gtgatgcatt ccggttattt gaatgatggc gtagccgaag cacaacacca gcgacattct
    24241 taccgcatga cggaagaggg cggattccgg cgtgaagtga cggctaagac gcagccagat
    24301 atcactcaat ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat tatggggcag
    24361 tgcctgggcc tgttctgatt caattgttgc cagttgggca tcaatggcgc gtaaattgtt
    24421 cagcaaaaat cccagtgttt tgagtaaatc ggcaggcgcg ccgttatcgc gcatccgctc
    24481 cagggcagca tcaatatgcg taaaagcgcg ctcaaaatgc ggatcatgtt gataaggctg
    24541 acgcagcaaa atacagcgtg acagttgctg gcacgcctgg ccctgcatcg acatcagccg
    24601 ctgaaaacgg aacagcacgt cgctgtggcg aaaatgttca cgcaatgttt gatactgaat
    24661 atgagaagag ctggcacgct cgtgaatatc ctgtgcgaca aagtaataat gcagcgtgcg
    24721 acgcgttccc cgttgaccac gatcgccacg taagcgggtc agcagcgata gtttcgtctg
    24781 attcaatgtc gccatcagct gaccgttggc gagagccaaa tcgtacagcg gtgcctggct
    24841 ttcatcttca atatcaggat caaacatgcg cgacttgagc tcaagataac gcgccagttg
    24901 ttcatagcaa cgcgccaggt tgtcctgcag cgggcggacc gggaacagca gatgaccaat
    24961 aagtgtcagg acgttgtacc agacggcacc ggccagcaga tacatcggct gctgatacca
    25021 gtgctcatac agtgatgttc ccaacatagt gtaaatggcg atcagcaatg caccgaaggc
    25081 aattgttgca tagcgttgac ccagaccgcc gagcaaaatg aagccgctgg tagagagcgt
    25141 taagccaatc gcaaatagcc agggccaggg aaacagcaat tctactgagg ccgaggcgat
    25201 aaaaaagcag aacagcgtaa tgatgaggtt acgcaaacgt cccgccagtc ggtcatcgag
    25261 atcggtcagc gccgctgcca ccatccccag cgtcagcgga atcgtcagtt ttacatcacc
    25321 cagccaccac ggaaacgctg tggttccaca aagcgcaata aaaatacgcg cgtaatacag
    25381 ccaggcgctg ttccaggtat agcgtttgag caaaggactt agcataaagg ccatattagt
    25441 ggattattca aaacgacgac gtgcattggc ttcacgcgca gcttgcgctg tttctaccga
    25501 taccacgcga cgaccaaccg gccatagtgc aatagcggca attttaaagt tcgcaatgcc
    25561 gacaggaatg ccaatgattg aaatacattg tgcgatgccc gttgcaatgt gcatcaggca
    25621 taaccaccag ccaaagaaaa tcagccagaa aatattcaat accgtaccgc cagtattcag
    25681 cagcacattt ttgccagccg ggttcagttc atcgacatga atagcttcat tgccataagg
    25741 caccagagac agtttagtga tctcccagca ggatcgtgtc agcggtaagg taaaaatcag
    25801 cacaatactg accagagtcg ccaacaacca gcccagagtg gtggcaaatc cgccaagcac
    25861 aaagttcaga atgttcaaaa cggtacgcat aaaacctcgc tttactgtgg ttttcagtaa
    25921 tgggcggcaa ttgtaacgtt tttttgggct ggagcacgtt ttctctgacg gttacactga
    25981 taagaaataa tttcgtgtgg atctacagag tcatggaact gaaagcgaca acgcttggaa
    26041 aacgtctggc acagcaccct tacgatcggg cggtgatcct caatgccggg attaaagtct
    26101 ccggcgatcg ccacgaatac cttattcctt tcaatcaatt actggcgatt cactgtaagc
    26161 gcggtctggt atggggcgag ctggaatttg tactgccgga cgaaaaagtg gtgcgtctgc
    26221 acggcaccga atggggcgag acgcagcgtt tttaccatca tcttgatgct cactggcggc
    26281 ggtggagtgg cgagatgagc gaaattgcgt ctggtgtttt acgccagcaa ctggatttga
    26341 ttgccacgcg cactggagaa aataaatggc tgacgcgtga gcaaacctct ggcgttcagc
    26401 aacaaatccg ccaggctttg tcggcgttgc cgttgccggt taaccgactg gaagaattcg
    26461 ataactgccg tgaggcgtgg cgtaaatgtc aggcctggtt gaaagatatt gaaagcgctc
    26521 ggttgcagca taaccaggcg tataccgaag ccatgcttac cgagtatgcg gattttttcc
    26581 gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg ggcagtcgtt aatggcgagc
    26641 attctctgtt agtgctggca ggtgcaggaa gcggaaaaac gtcggtgctg gtggcccgtg
    26701 caggctggtt gctggcgcgt ggtgaagcgt cccctgagca aattttattg ctggcgtttg
    26761 gtcgcaaagc cgctgaagag atggacgagc gtattcgcga acggctacat accgaagaca
    26821 ttaccgcacg cacgtttcat gcgctggcgc tgcatattat tcagcagggc agcaaaaaag
    26881 ttccgatagt cagcaaactg gaaaatgata ccgcagcccg tcatgaactc tttattgctg
    26941 agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa gggctggcgg caatggctga
    27001 cggaagaaat gcagtggtca gtgccagaag gtaacttctg ggatgatgaa aaattacagc
    27061 gtcgtcttgc ctcacgcctc gatcgttggg taagtctgat gcggatgcac ggtggtgcac
    27121 aggcagaaat gattgccagt gcacccgaag agattcgcga tctgttcagt aaacgtatca
    27181 agttgatggc cccgttatta aaagcctgga aaggtgcgct gaaggcagaa aacgctgtcg
    27241 atttttcggg ccttattcat caggcgattg tgattctgga gaaaggtcgc tttatcagcc
    27301 cgtggaagca tattctggtt gatgaatttc aggatatctc gccgcagcgg gcagcgttgt
    27361 tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt cgccgttggt gatgactggc
    27421 aggcgattta ccgattcagc ggtgcgcaaa tgtcgctcac caccgctttc catgaaaact
    27481 ttggtgaagg cgatcgctgt gatttagaca cgacttaccg ttttaacagt cgtatcggtg
    27541 aggtggcaaa ccggtttatt cagcagaacc caggccagct gaaaaagccg ctaaacagct
    27601 taaccaatgg agacaaaaaa gccgtcacgt tattggatga gagtcaactt gacgctttgc
    27661 tggataagct ctctggttat gccaaacccg aagagcgcat tctgatcctg gcgcgttacc
    27721 atcacatgag gcctgccagc ctggaaaaag cggcaacacg ctggccgaag ttgcaaatcg
    27781 actttatgac cattcatgcc agcaaagggc aacaggcgga ttacgtcatc atcgttggct
    27841 tgcaggaggg aagtgatggt tttccggctg cggcgcggga gtcgattatg gaagaggcgc
    27901 tactgccacc ggttgaggat ttcccggacg ctgaagaacg gcggttaatg tacgtggcgc
    27961 tgacccgggc acgccatcgg gtatgggcac tgtttaacaa agagaatccc tctccctttg
    28021 tggaaatact gaaaaatctg gatgtgccgg tggcgagaaa accgtaagaa acaggtggcg
    28081 tttgccacct gtgcaatatt acttcagacg gtccgcgaga taacgctgat aatcggggat
    28141 cagaatatcg accgcgtcgt tgaaatgcgg cgactggata atgaagtctg ccgttgccac
    28201 gttggtggcg accggaatgt tccataccgt cgccagacgc agcaaggctt tcacgtcagg
    28261 atcgtgcggc acggcattta gtggatccca gaagaaaatc aatacatcaa ttttcccttc
    28321 tgagatcaat gcgccaacct gctggtcacc ccccattggg ccactcaaca tcgcgttgac
    28381 gttcatgccg gtcgcgcggg aaattaagtt accggtagtg cctgttgcat acagtacgtg
    28441 ttgttccagt aacggttgat gccgttccac ccagctcatc agcatttgtt tgcagtgatc
    28501 gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg cgagtcgtca gttccataat
    28561 gtacatccgt agttaacttt cctacagatt actgtaagca cttatcgctg caagataaag
    28621 accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt ttccgcccat
    28681 tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa accaatactt cagccgttgt
    28741 tctgtgagca cctgagactg cggtggaata gcatccagct cggaaacacc cgaaagcagc
    28801 gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt cgcggatttg
    28861 ttatagcgtt caacctcgac ctcataatca atcgtttttg ccgttgaggt aatggcgaga
    28921 atatccagct ttaccggaat cggcgtcgca tcgccatcca acaattcaag gcgcggcgcg
    28981 gcatcaaaat ggttagcttc ccgctcattc tccaggcgag gcaggcgaaa attgacctgg
    29041 ttgatgagct gggtattaaa actgaccacc agcggtgggg agatatacag ccgttcttca
    29101 ctattggaaa gatgaatcgt cttctcaacg cgaaacacta actgatgcgg tccattatcc
    29161 agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa ccttcttgcc gtcgagtacc
    29221 agaagatcga catcggttga aagccgcagc gtggtggcaa aaacggatac cggcaaacat
    29281 aatgcaatca aggtggtcac gatgccggtt ttcataggta gctcctgtca aaagaccgcc
    29341 cgaataataa tgtgtcaaaa tttttcttta atcatactta ctaacatata gacatatttc
    29401 ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc tttgtcgtac
    29461 actttgttaa acagtctgga ggaaataatg aaagaaaccg atattgcagg cattttaacg
    29521 tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc cagctatcgc
    29581 gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc aaaagttgcc
    29641 ggcaaaacgc tgctaggcca gcaggggtat ggcacgctgg cagacgttcc ggaaaaggtc
    29701 gatatggtcg atgttttccg taattcagaa gcggcgtggg gcgtggcaca ggaagctatt
    29761 gccattggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca agcggcagta
    29821 ctggcacggg atgccgggtt gaatgtggtg atggatcgct gcccggctat tgagatccct
    29881 cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg ggatttgttc
    29941 agggattagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg cgccagctca
    30001 tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg ggcttcagcc
    30061 aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata ccacggagcg
    30121 gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag cgaataaacc
    30181 gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg
    30241 ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg
    30301 gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct
    30361 tccggatagg tagcgatcac cggatgatcg gccgcctgac ggaactgctc tataaattgt
    30421 acatcacgac cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc
    30481 atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcaa ctgaatcgcc
    30541 agcatgttga tgtctttata gccacgacac gcgcccatca actggctttt attctcaaca
    30601 aacttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataagtacgc
    30661 agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagt
    30721 tcaacgttct gccgtgcaat atccagcgct tcctgggagg tatcaacgct gacaacctgg
    30781 ctgcaaccgc ccatcagtgc cgatgcggcg aaaccaccgg tataggagaa acagttcagt
    30841 acgcgtttat tttcaacgta gcggcgggta gctaggcggc tgtctcgctg gtccaggtag
    30901 tagcccgttt tgtgtccgtg ctgtatgtcc accagcagct tcatgccatg ttcttcaatc
    30961 ggcagcaggg caggcggcaa ctcgccggtg acggggccct gggtcagctc catcccttct
    31021 tttttacgta ccgcgacgtc gctgcgatcg taaatcgcac attccgggta cagcgtttgc
    31081 agggcgctaa ttaatgccgc gcgctgatac tctgccccag cactcagcag ttgcagcacc
    31141 agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc accggcgatt
    31201 aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt ttgtgcttgt
    31261 tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa cgtccagacg
    31321 cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga
    31381 tcaacaatat caatggtttc accgaggctg gctttacctt ccatacgggc aacggccccg
    31441 gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa
    31501 cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt gtccggagtt
    31561 ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc
    31621 ataattgcct gggtttacgg gcgagttcag ggcgtaggat ttcgctatac cacgcagtac
    31681 gaagcgaaaa aactcgggtt aagcgggtac gccaaaaatc ttgacgatgg cagcgtggaa
    31741 gtggttgctt gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt aaagagtggc
    31801 ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggtgaa
    31861 ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc agcaattttg
    31921 gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct gttacctttt
    31981 tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg agaagtattg
    32041 aattccagat agaaatcacg cacaaaacgc accacttccc agtgttctgg cgacagcgaa
    32101 atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct gctttctttg
    32161 agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact
    32221 accttaatca gactgccggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat
    32281 gacagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg
    32341 acgaagatgt tgtacagaga aacatacagg ctaaccgtgg cacgaatata gttcgtttca
    32401 ccgccatgaa tgatgttgct ggtttcaaac aaaatagcgc cagaggagat caaaatgaag
    32461 accgcgctga tcgccagatg cagagcaggc agctgcagga agatattagc aaccatacca
    32521 atcagcacca ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg
    32581 gtggtcagca catatgcaga gcagcagaag aacactaacg ccgttccgcc cagtgccata
    32641 gcgattacgt cacccattcc ggcagacaga taggtgttca gaattggtcc gaggatataa
    32701 cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccgtttta
    32761 taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc
    32821 agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc
    32881 agaaaatagg tattacgcag cactttatgg gtgctaagca gtgatgtacg gtcatgtgaa
    32941 gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca
    33001 taatgataat gcgtaagggc acccagtagt tttacccatc tttacgcatt tgatctggaa
    33061 caggtttaac agcggattat caggtcatta agcaaatata acgccctgag aatttcgaca
    33121 ggcaaaagaa aaaggggtta gcatttagct aaccccttat cttatttggc ggaagcgcag
    33181 agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccgaaga aaaacaaata
    33241 aaaatcagtg taatacgtga aattgctgaa aagttaatca aatttaacac caagaaaaat
    33301 caaggtatta ggaagttgct catagctagt ataccaagtc tttttgccag aaatttttct
    33361 ggattgtcta cttgctaacc acgagcggca aatccagtgt tggtgtgatt tttgttttac
    33421 gatcgtaaat caacacctga ttttctgttt tatgcccgct gaaaatttgt ttgtcgcgac
    33481 tgctgccttc gtaatctgag atccccttgg cttttatgtc gtgaaaattg cacccgaacg
    33541 gaacgccaac tttttgctcg gctgcgcgtt tagcctgatt ccaccagtta ttcagcgttt
    33601 tagctatgac tttcccgcct ttggttgtat tgatcacata ttcgcatgta caggaagata
    33661 catttcgggc taactggatc gctgtacgta atcgcggtga ccattccttg atttgtttgg
    33721 tgccggtttt gttttgctca atgtagatcc ctttatccat aatatcctgc catttcaact
    33781 caagcacatc accgagtctt gccgcacaga gataggaaat ctccattgca atgcgtaact
    33841 gtggaattgc ttccgcatat atcgccgcat actgttcatc ggtgatgtaa acagtgcggg
    33901 ctttaagaga gaattttctg actcctttgc atgggttatt cttcacatac ccacgctcat
    33961 atccccaccc gtatactcga ctcaggcttg ccagttcatg atttgcctgg gtcttgctct
    34021 caagccctcg tttatccatg aaaattctta cctgctcagt tttgacatta tcagcaagca
    34081 cttttccgaa taccatcagc aacgccttct gatgttgtcg ataatctttt tgggttcggg
    34141 gggccagttc tgtaaatgca ggggagtcca taaacatgtg ccacaattta gctacggtca
    34201 ttatgttgtg gagttttgct ttttccagtt cataattttg ccagacttta gctacgctgg
    34261 ttttccgcac tcttcctagc cctatacttc ttgtacttcc ttcgggtttc cacacgtaac
    34321 tgtaaccatt cgatctaacc cgcggtggca gtacattatc ttttttattt tttcttggtc
    34381 ttcccattgt tcagcgcctc aaaatcgggt tcagcagaaa ccagttcaga tgtttttggc
    34441 atcgttgtca gaccgtgtgg aatatccctg cggagaacta tgggttcgtt tttagggccg
    34501 attacaaatg ggatgccgtg cagtcttaac tggtgttgct gttttgtgta tcgctcgtat
    34561 ttcgtgatct ctttaatctc tgctggagat agagttaatt cgtacatgtg gtcacgtgcc
    34621 tttacagcat gaccgccgcc actatagctg gtgggcggcg atatgggttg aacatcaaaa
    34681 atcagcctga ctcgggagca gtttttgcca gatggctgaa acgtatttcg cctggtaacg
    34741 tgcatcgttc agagcgttat ggcgttcacc ttcgaatgga atagtcgttc ttgcgtcgaa
    34801 atccatcatc agtcccagag caaccatcgt tcttacatcg cgatcattgg tgtaacgcca
    34861 tgggcagggg atcccctgcc gttcatatga acggcgtaaa atcacgttgt cgaaagttgc
    34921 accgttaccc cagacctgaa caaaaaattc accggagttt tcgtcgataa attcccgtaa
    34981 ttgcagcagt gcatcatcta acgggatttc atcggtaaga atggcggatt gcgcttcgcg
    35041 tgactgtttc agccaccact taatggtgtc ccgatcaatg actccgccag cagtttccag
    35101 atcgatagtt ttgctgaatt ctggccccat ctctccggtt tgcggatcga aaaatattgc
    35161 acctattgag ataatcgggg catcaggatt ttttcccatt gtttcaaggt caatcatcag
    35221 atggtgccac aacctgctgg tggatgtaat ttcacgatga ccgttcacct taattaaggg
    35281 atttgctgtc tcgccacttt cattatctgt ggtgtggtag tgatcgccgc cactgccttc
    35341 catttcctcc tgagcttcag cctgattttc ttcatcgaat gtttcctggc aggttgcgtc
    35401 gcccatcacc gcgccacaat cagggcagtt gccgccacca ctctgaccgc aggcggtaca
    35461 gactttttcc tcttcctgtt gcgctactgg ttcggattgt ttcgtttctg gctcgtcttg
    35521 taacgcattt gggctgtttt gttccgcttt ttggtcgttc tgttccgttt ctgactggtt
    35581 ctggttcaca gaatcgcagg tttcaatccc cttcacccat ttcggatcat tcggatcgct
    35641 aatcccttca acaaattcac cacgtgatgc agcaagcaat ttatcggcgt caggctggct
    35701 gatattggct gcctgcataa ttttgtttac ttcgtcagcg gtaactttta ccggctccgg
    35761 ttgtacggaa tcttcagcgg tatccacatt ttgcggtaag tccgtgtatg ttccgttttt
    35821 gcgggcaaga tattcttctt tcgtgatttc agtagccccg gcagccagcg ccttgtccag
    35881 accagaaagt ttgtttgcgc gaccgtattt ttcgccatcc ttgtcggtga agaggaagta
    35941 gaacggcccc tcacgctcta cagatggttc gacttccact ttgcattcgg ttttttcgtt
    36001 gtcc
//
LOCUS       DAZUGK010000023        35428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZUGK010000023 DAZUGK010000000
VERSION     DAZUGK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.023"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHY5W_001029"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHY5W_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFS4445966.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVTTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHY5W_001030"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHY5W_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFS4445967.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHY5W_001031"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHY5W_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFS4445968.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHY5W_001032"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHY5W_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS4445969.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHY5W_001033"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHY5W_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFS4445970.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHY5W_001034"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHY5W_001034"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFS4445971.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEALPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHY5W_001035"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHY5W_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFS4445972.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLGRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIQNADLSTYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHY5W_001036"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHY5W_001036"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFS4445973.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHY5W_001037"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHY5W_001037"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFS4445974.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPRGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHY5W_001038"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHY5W_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFS4445975.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHY5W_001039"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHY5W_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFS4445976.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHY5W_001040"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHY5W_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFS4445977.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATDKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHY5W_001041"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHY5W_001041"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFS4445978.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALSNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHY5W_001042"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHY5W_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFS4445979.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHY5W_001043"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHY5W_001043"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS4445980.1"
                     /translation="MSHIKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHY5W_001044"
     CDS             complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHY5W_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFS4445981.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHY5W_001045"
     CDS             complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHY5W_001045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS4445982.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15607..16794
                     /gene="metC"
                     /locus_tag="ACHY5W_001046"
     CDS             15607..16794
                     /gene="metC"
                     /locus_tag="ACHY5W_001046"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFS4445983.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAICELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16934..17593
                     /gene="yghB"
                     /locus_tag="ACHY5W_001047"
     CDS             16934..17593
                     /gene="yghB"
                     /locus_tag="ACHY5W_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFS4445984.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHY5W_001048"
     CDS             complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHY5W_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFS4445985.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDQHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLIGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRTMQGN"
     gene            18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHY5W_001049"
     CDS             18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHY5W_001049"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4445986.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHY5W_001050"
     CDS             19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHY5W_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFS4445987.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21021..21947
                     /gene="yqhG"
                     /locus_tag="ACHY5W_001051"
     CDS             21021..21947
                     /gene="yqhG"
                     /locus_tag="ACHY5W_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFS4445988.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTLEQTVRHIYQNYKSDATAPY
                     FGETGERAITSVRIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21998..22255
                     /gene="yqhH"
                     /locus_tag="ACHY5W_001052"
     CDS             21998..22255
                     /gene="yqhH"
                     /locus_tag="ACHY5W_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFS4445989.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTHLDAQDYFDCLRCLRMYAE"
     gene            complement(22297..24516)
                     /gene="ygiQ"
                     /locus_tag="ACHY5W_001053"
     CDS             complement(22297..24516)
                     /gene="ygiQ"
                     /locus_tag="ACHY5W_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFS4445990.1"
                     /translation="MSSISLNQPDRDLFSWPQYWAACFGPASFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDSVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQPQTPATAKKAPSMQSRPVNTGAKKRPKAAVGR"
     gene            complement(24627..26039)
                     /gene="ftsP"
                     /locus_tag="ACHY5W_001054"
     CDS             complement(24627..26039)
                     /gene="ftsP"
                     /locus_tag="ACHY5W_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFS4445991.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26114..26851)
                     /gene="plsC"
                     /locus_tag="ACHY5W_001055"
                     /gene_synonym="parF"
     CDS             complement(26114..26851)
                     /gene="plsC"
                     /locus_tag="ACHY5W_001055"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFS4445992.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27085..29343)
                     /gene="parC"
                     /locus_tag="ACHY5W_001056"
     CDS             complement(27085..29343)
                     /gene="parC"
                     /locus_tag="ACHY5W_001056"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4445993.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPITIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29481..31088)
                     /gene="ygiS"
                     /locus_tag="ACHY5W_001057"
     CDS             complement(29481..31088)
                     /gene="ygiS"
                     /locus_tag="ACHY5W_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4445994.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEIMDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSDENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31221..31616)
                     /gene="mqsA"
                     /locus_tag="ACHY5W_001058"
     CDS             complement(31221..31616)
                     /gene="mqsA"
                     /locus_tag="ACHY5W_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HFS4445995.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31618..31914)
                     /gene="mqsR"
                     /locus_tag="ACHY5W_001059"
     CDS             complement(31618..31914)
                     /gene="mqsR"
                     /locus_tag="ACHY5W_001059"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HFS4445996.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32119..32601)
                     /gene="ygiV"
                     /locus_tag="ACHY5W_001060"
     CDS             complement(32119..32601)
                     /gene="ygiV"
                     /locus_tag="ACHY5W_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4445997.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTKQRLETDVYVPLA
                     "
     gene            complement(32654..33046)
                     /gene="ygiW"
                     /locus_tag="ACHY5W_001061"
     CDS             complement(32654..33046)
                     /gene="ygiW"
                     /locus_tag="ACHY5W_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFS4445998.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33198..33857
                     /gene="qseB"
                     /locus_tag="ACHY5W_001062"
     CDS             33198..33857
                     /gene="qseB"
                     /locus_tag="ACHY5W_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFS4445999.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33854..35203
                     /gene="qseC"
                     /locus_tag="ACHY5W_001063"
     CDS             33854..35203
                     /gene="qseC"
                     /locus_tag="ACHY5W_001063"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFS4446000.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTLDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35285..>35428)
                     /locus_tag="ACHY5W_001064"
     CDS             complement(35285..>35428)
                     /locus_tag="ACHY5W_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFS4446001.1"
                     /translation="DRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVCSFVICL
                     VCG"
BASE COUNT         8734 a         9421 c         8901 g         8372 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctagttttgt ccagcacttc
      241 ttcatgatgg ctgaccaggt cgatattgct accacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt gtcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacat gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtaataatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtatcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgtaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 catccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgctggg cgttactggt gcagagtacg acgcctggct gatacgcata
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg
     2401 gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgagtctgg ttcgatcctg
     2461 ctttatctgg cagaaaaatt tggctacttc ttgccgcagg atctggctaa acgtactgaa
     2521 gcaatgaact ggctgttctg gctacagggt gccgcgccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaaacgtc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaatgt ggtattaggc
     2761 ggcgtatatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt gcaacgctgg
     2821 gcaaaagaag tgggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataagc gtcaggggta agggttagcg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagaccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag tagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggca
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3841 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcaaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggagggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcatcgct tcgaccgtcg
     4261 gcgtcaagcc gatgtgcggc tccagtgatt ccggaattac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag tagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttgctga tgataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     4981 cacaaaggtg gtgtggtcgc ctttgccgat attggtgatc agtgcactgt attgattttg
     5041 caggatatcc tgcaattcgc agcagtgaac ggtacgacca ataatacggc ccagcgtaga
     5101 gtgcagttgc gccacttcta gcgtgttacc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgtcc tgccgccagt ttcaccagca tgttagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggcgat ttcacccagg agtatttacc
     5281 gtcgtcagac caaccgtcgt aagccggaat ggtggtgcct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaagagt gcttcgcgct ttcctgaatc cctttgatca ggtattcatc
     5401 agagtgcgaa gtgatcggac ggtaagtcga tagatcggca ttctggatgt agccgccagg
     5461 gaacaggaag ctgccgtttt tgctgtcagt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccacgcccca gccattccgg gtagaacgca gcgataaccg cggtatcgac
     5581 cttgtaaacc tgctcaacaa agtcactcag cttgtcgatg aaggacttga tgtacatcag
     5641 acgctcaagg ttcagcacgc ccagaccgtc gaggttgatc gggttagcga ccccacctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggtttgcgtc
     5761 acgctggcac tccagcgctt gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcaggtgcca
     5941 ggtcgaaaca cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg ttgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttttgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcagcc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga aaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacgccagtg ccgctggtcc acacctgaat gatgttattg gtatacggcg atagtttgtc
     8281 gttgttcgac caggtctgtt cccctcgcgg gttacgttca gggaagttga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca acgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcccgc agagacgttg
     8641 ccgccttctt tagcgttaac gtccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaggctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggtgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg tcatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    10501 caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgtc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcattacccg gataaccgcc aacggaactt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 accaccacca cggctggcgt tttgccgctc atccttgcgg gtttcaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat ctccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt tgggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg
    11581 gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    11941 tggctcaggg attgctgact ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggcctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attgattggt gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgagtaatct gacgtttagc accgaggagc
    12241 tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccattaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatcgag ttgtgagacg
    12601 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    12901 gcaacagcaa atcaacatat ccgttaaacc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa cgatgtctca tataaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt
    13381 tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcaa gtatcatcac cacttcgtca atccaggcat accagccaag tccgcattta
    13621 ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcacta
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg
    13801 aagcgtgcgg ggcaaccggc ggaactggcc ccagtatatg tttatctggc aagtcaggag
    13861 tcgagctacg tcaccgcaga agtgcacggt gtgtgcggcg gcgagcattt aggttaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    13981 gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta
    14041 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14101 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14161 tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14221 gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt
    14281 aatcgacggt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14341 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14401 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14461 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14521 tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14581 tatcatcgag gttttcgtta agatgcattg ccatcggaca ttatcctgca cgtaattttt
    14641 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14701 gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg
    14761 caaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14821 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtagtctgc gtttgcgcga
    14881 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggcgaaa
    14941 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15001 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15061 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15121 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15181 gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg
    15241 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15301 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15361 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15421 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15481 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15541 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15601 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15661 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15721 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15781 cgcggaacgt taacccattt ttccttacaa caagcgatat gtgaactgga aggtggcgca
    15841 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    15901 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15961 agcaaaatcc tcagcaaact gggcgtaacg acatcatggt ttgatccgct gattggtgcc
    16021 gatatcgtta agcatctgca gccaaacact aaaatcgtat ttctggaatc gccgggttcc
    16081 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16141 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16201 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16261 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16321 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16381 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggctga atggctggca
    16441 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    16501 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16561 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16621 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt
    16681 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16741 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16801 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16861 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    16921 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16981 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17041 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt
    17101 ggctggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17161 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17221 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcatcagc gcgccacctg
    17281 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17341 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17401 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17461 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17521 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17581 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17641 cctgcatcgt ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17701 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    17761 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17821 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    17881 tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17941 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18001 cgccgcaagg tccgatcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18061 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctgat
    18181 cttcgccaat atccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18301 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18361 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18421 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18481 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18541 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18601 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18661 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18721 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18781 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18841 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18901 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18961 aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg
    19021 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19081 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19141 tgccagcaac cggttcagaa tccaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19201 ataagcaggc gttccattca gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19261 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19321 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19381 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19441 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19501 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19561 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19621 ccaagcgcgc taagctgctg caatatgccg aacgcgtctg gaacatcact gaaggttccg
    19681 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19741 tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19801 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19861 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19921 catttcgtcc agacttaagt tcacaacacc tcaccggatc ctgctccggt gagttcatat
    19981 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20041 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20101 agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctataaga acgaagaagg
    20161 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20221 gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20281 acagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20341 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20401 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20461 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20521 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20581 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20641 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20701 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20761 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20821 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    20881 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20941 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21001 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21061 ttaccgtaca cgcacagccc ccctcacaga ccttagaaca aacagtccgg catatttatc
    21121 agaactataa atcagatgcc accgcccctt attttggtga aaccggagag cgggcgataa
    21181 cttctgtgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggtaata
    21241 ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag
    21301 aaagcgtagc gataaccgaa actgacgccg atcatgccga tgccgttgtg cgctttcgta
    21361 tctttaaaga tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc
    21421 gttgggtcat tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg
    21481 agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg
    21541 ttgccgaact ctttgaacat attgccgatt atagctggcc gtggacgtgg gtggtttccg
    21601 actcttaccg ccaggcggtc aatgccttct ataaaaccac cttcaagacg gccaacaatc
    21661 ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg
    21721 aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg
    21781 cccgcattca tattcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt
    21841 tacaacggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc
    21901 tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata
    21961 acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg
    22021 agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca
    22081 actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa
    22141 agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcatct
    22201 tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa
    22261 aaatccccgg cagcatgtca gttgccgggg atttttttaa cgtccaaccg ccgctttagg
    22321 gcgtttcttc gcaccagtat tcaccggacg agattgcata gacggcgctt tttttgccgt
    22381 agcaggagtc tgaggctggg tcgccatcgg cgtatgtttc gtcaacgctg gacgagtatt
    22441 gcggttctga cgacgagcct cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc
    22501 gcgacggctg ccaatcagat gctttttacc cattgcttcc agcgcctgac ggattaacgg
    22561 ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct gtttgtcgcc
    22621 cttcggtacg aagacgtctt cactcttata accaatcttc gccagcgggt ttttcccggt
    22681 gtaatacatg gtggtcgagt tagccagtgg cgatgggtag aagttctgta cctggtcgag
    22741 acggaaacga tgctttttca gccacagcgc cagattcacc atatcttcat cacgcgtacc
    22801 cgggtgcgcg gagatgaaat aggggatcag atactgctct ttacctgcct gtttcgagta
    22861 ggtatcgaac agctctttaa agcggtcata actgcccatg cccggcttca tcatcttcga
    22921 caacggccct tcttcggtat gttccggagc aatcttcaga taaccgccga cgtgatgggt
    22981 cgccagttct ttgatatagc gcggatcttc cacggcgatg tcataacgta cgcctgaggc
    23041 aatcaggatc tttttaatgc ctttcagatc gcgagcgcgg cgatagaggt tgatcgtcgg
    23101 ttcgtggtta gtgtccatat gcggacaaat atccgggtaa acgcaggaca aacgacggca
    23161 ggtttgttca gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc
    23221 gagatcggaa attacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt
    23281 aatgatcgaa tcttcggaac ggctctgaat aatgcgccct tcgtgctcgg tgatagaaca
    23341 gaaagagcag ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga tcatttcgta
    23401 agccggaata cgggcattgc cataggccgg atgtggcacg cgcttgtatg gcagcgcaaa
    23461 aacgctgtcc atctcttcgg tagaaagcgg aatagcaggc gggttgatcc acacatagcg
    23521 gtcgccgtgt ttttgcatca atgcgcgggc gcagcctggg ttggtttcgt ggtgcagaat
    23581 gcgcgaagca tgagcgtaca ggactttatc gcccttcact ttctcgaaag aaggcagcaa
    23641 tacgtaggtt ttttcccacg gtttcgggcg cggcggctgc acagttacag ctttcgcttc
    23701 ctgctttttc ggcgcaacag gtttgttatc cgcacacggc aaatcttcgc catacggatg
    23761 cgggatcggg tcgattttcc caggggtatc aagacgagta gaatccacac cgctccagcc
    23821 tggcaacgcc tcttttacga taatcgcagt attacgcaca tcgcggattt cactaatcgg
    23881 ctcgcccatc gccagacggt gcgccacctc caccagcgga cgctcaccgt taccaaacat
    23941 cagcatgtcg gctttcgaat ccaccagcac ggaacggcgc acgctatcgg accagtaatc
    24001 ataatgtgcg gtacggcgca gactagcctc aataccaccg aggatcaccg gtacatcttt
    24061 ccacgcctct ttacaacgct gggtataaac cagtgtggcg cgatccgggc gcttacccgc
    24121 gacgttatcc ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg tataacggtt
    24181 gatcatcgaa tccatgttgc cagcagtaac accgaaaaac agattcggtt tacccagacg
    24241 cataaagtcg tctttgctgc tccagtctgg ctgagcgatg atcccaacgc gaaagccctg
    24301 cgcttccagc atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc
    24361 gccagtaacc aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga
    24421 catcggcaaa aacgatgctg gtccaaaaca ggcggcccag tactgcggcc aggagaacag
    24481 gtcgcgatcc ggttggttca gggagataga gctcattttg cttccagaaa tgataaaaaa
    24541 ataatcaaag gccggggatt ataagccgga acgaaagaga aatcgaaaag tattccatac
    24601 tcgccctcct cgggcgagta tgaagattac ggtaccggat tgactaacag ttgcccaatc
    24661 gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag
    24721 gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac ggtatctttc
    24781 cagcctctgt cttccgggaa cggcatcgca ccattcacgt tacggatctg gaacatcacg
    24841 ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct
    24901 tgctgcgcgg tgacatcaat acggttgacg tcccacagtt gtccattaat acccgggtca
    24961 tcacccagac tgatatcgcg actgcgaatc ggcgaaccgg ccatgatttc agttggcagc
    25021 aggcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc
    25081 accagggtag aaaccagaat gctggatggc tcaaagaagc cacgaatacg atcaacaatg
    25141 ctcgccgctt cgccacaggt gatcgacact tcatcaccgt tgctcatatc caccagaatc
    25201 tcgcggcgct cgcccggtgc caacgaaagt tgcttcaccg atacaggagc aggcaggaat
    25261 ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag ttgatagcga
    25321 cgagagttcg acgcgttcag cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac
    25381 gggctttgta caccgttaac cagcagcgta tcaccaacaa agccgccgct tcccggttcg
    25441 ttgtattctg gcgtaccaaa gttatccagc cgtttatcct ggataatgac cggaaaatca
    25501 tccacaccat aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac
    25561 attccggcaa ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac
    25621 cacagagtag ctgcgttctg gcgaataggc agtacgggtg cccagtcagc gtttggcgac
    25681 atcatccgtg ccggaccgcc catcagcggg cctggtacct gtagcccggc caccgtcatt
    25741 gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg
    25801 cggatagtcg gccccaggta acgaccattg attccccaga ccgacgcgcg tgtccctggc
    25861 gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca
    25921 agcagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca
    25981 gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata
    26041 tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat
    26101 acagattgcc gacttaaact tttccggcgg cttcgcgttc tgcgacttct ttatcgagct
    26161 cggcgatttt ttgttccatt atcgaacgac aatgggcagc cagctcacga acctgatctt
    26221 tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgca
    26281 gtcgattaag attaatctta ttcgaagttg tagagacgca cacgggaata atcgggacgc
    26341 ctgctgcaat tgccgcgtga aatgctccag tcttgaacgg tagcaggccg cgaccacggc
    26401 tgcgggttcc ttccgggaac atccaaatgg aaatacggcg ttttttgaaa tgattcacta
    26461 cttccgcaat agtgccgtga gctttagtgc gattgtttct gtcgatcaat aagttgccgg
    26521 ttaaccagta caactgcccg aagaagggga tccacagcaa gctcttttta cctaccgtca
    26581 ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag
    26641 cgatatagat agcattgccg tagctttcag catctgcagg tttgcggcac tcaactttca
    26701 ggccgaacag cggcgcaaga cgaccaaaca tatgcccaaa ggtggccaca tgtttcgggt
    26761 tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca
    26821 cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc
    26881 attgtacgtc acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt
    26941 attgttaatc gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg
    27001 ggttgaatac gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga
    27061 ggaaacagcg ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg
    27121 agagtcgatc tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc
    27181 acgttcgcca gtgactttct gtagctcttc cggacgcagt ttaattttgc gtttcccaac
    27241 atgaatggtc agcgtgcttt gcggcggcag aacgtacagt tgcgccagac catcctcgcc
    27301 acgcgcggct tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg
    27361 cggcagatca cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat
    27421 atcggaagca tcttcaatta ccaccggcgg cataacatgg gcattttccg gtaaggtgat
    27481 caaagcctta cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta
    27541 acccgcatcg gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac
    27601 ggtcgcccca ggcggcaacg ttaatttgcc ggtgagcggt tcgccctgac cacgcgccga
    27661 cggcagcgta atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccgg
    27721 ttggttgctc ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc
    27781 cggcgcgtcg atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat
    27841 ggtgataggt tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc
    27901 ctgcaacggc gaacgacgat cgtcaccgta ggcttgtgcg tctgcctgca gttctttctt
    27961 cagcaggtta ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt
    28021 ttccagctca ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag
    28081 tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc
    28141 cggtttcggt tcatcttcat tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc
    28201 caccagcaaa ccttcgagga tatgcaggcg cttgaggact ttctccagac gatagttcag
    28261 tcggcggcgc acggtatcgc ggcggaacac cagccattcg gagaggattt ccagcaggtt
    28321 tttcaccgcc ggacgaccat ccagaccgat catattaagg ttaatacgat agctcttttc
    28381 cagatcggtg gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg
    28441 cggcacaatc accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac
    28501 catcggcagc tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga
    28561 aacctgatgc ggcaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg
    28621 catacgcact gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat
    28681 aatttccgct tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt
    28741 ggttttcggc tggtcgatta atgcgattgc cgcctgagcc acttcacgca ggttatgcgg
    28801 tggaatatcg gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg
    28861 cagacgagca ggtagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc
    28921 agccgtcccc tgccccaact cgctcaatag cagctcggaa tatttcgaca accgggattc
    28981 ggtgtaacgc attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc
    29041 atcaaccagc ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca
    29101 ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt
    29161 tttaaatttg gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg
    29221 aacaggtttc agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga
    29281 gtagtttaag taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct
    29341 cattaattct gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat
    29401 actacattat ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg
    29461 ctccgacggc gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacataa
    29521 tcctggggat tatgcagcgg aaaaccgcca acgtagggtt taagcagttt gattaacggc
    29581 tgatagtaga taggaatcag cggtgcctgt tggttgatga tcacttctgc ctgctgatac
    29641 aacgcattac gctttgtcgc atcagtgatc tgcgtggcct ggtttagtaa ggcatcatac
    29701 tgcgcatttt tccagtgacc gacgttttcg tcactatcgc ttttgagcgt gttcaggaag
    29761 ctggaagcat cattatacgt cgcatcccac gactgccgag acagcatgaa atcaccggct
    29821 cgtcgggcat caagataggt tttccactcc attgtgcgca gcgtcacctg tgcacccagc
    29881 cattttttcc attcggaaga caacgctatc gcggtctttt catgcagatc gtacttgttg
    29941 tagaacagct caaagcgtag cggatgagag gcgtcgtatc ccgcctgttt cagcaaggct
    30001 tttgccatcg cgacgcgctc actcattggc ttttgcagtt catcgaacgt cgtcgcgcta
    30061 aagcctttta cctctggcgg cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt
    30121 tgcgcaataa gctgtcgatc aaccgtaaga tatagcgccc gacgcactcg cacatcgtta
    30181 aatggcggtt tctcaaggtt gaagttgtaa tattcgctgt tcagacgcgg aataattcgt
    30241 agctcgccag gcagtgattt ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga
    30301 tcgacctctc ccgcgcgata gcggttatag ccggtgaccg aattatccag cgccagatac
    30361 tcaacctgtt gcaatactgt atgttgcgca tcgcggtact ttggattttt gcgtgcagta
    30421 atcttttcgt taactaccca ctgatcaagc acaaaggcac cgttgtaaac catgttctct
    30481 ggcttactcc agctatcgcc atgtttagcg atgacatgat gagggaccgg gaacagcgtc
    30541 ggccaggcga gcatcgtcgt gaaccaagga accggctgct caagcgtaac ttcaagagta
    30601 cgatcatccg tcgctttgac acccagcgat gtaacatctg ctttacccgc aacaatagca
    30661 gcggcattgt taatgtgcgc ctgtgccaga tagccagcaa aagggcttgc cgttttcggg
    30721 tcaaccgcgc gctgccagcc gaggacaaaa tcctctgccg tcagaggctg accgtcggac
    30781 cactgcaaac cgctacgcag atggaaaata tagcgcctgc cgccgtccat tatctcccag
    30841 cgttcagcct gagcgggctg cacctggcct tcaccgtcca tccataccag accttcaaac
    30901 agatccagca caatctgcgc ggcagtattc tcctccacct tttgcgggtc gagcgtacct
    30961 gggtcgctat gattgttgta acgaaagact tgttgcggag cgagcggtgt gttggtggga
    31021 acgtcagcag cgtagagagg agccgcactt accaggctga ccagccataa taaatttcgc
    31081 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    31141 tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagaa aaaagcgagt
    31201 tgcccccgct tttccattaa ttaacggatt tcattcaata gttctggatg cttatccaga
    31261 acacgtaaaa gtttgattgt ggaaggatga ggttgggcat tgcctttttc gtaacgcgaa
    31321 aacgcattta cacctccccc aaaaatttcg cttgcctctt tttgtgtaag agagagcttt
    31381 tttcgaacct tcactataaa ttcaggtgcc actgtttcgg cattcaccga agcccgaaat
    31441 gcctttactt gcgccatgaa agcatctgac tcttctttat tcatgatgct ctcttcgcaa
    31501 tggacacaat ataaaccgtg gatacctttc aatactgttt ttcgtccacg gaaggtgtat
    31561 ggaatatctt taatgccaga aaccatttct ccctggtggc aaaccggaca tttcatatta
    31621 cttctcctta aacgagacga tcagtacgtc atgaattacc gtaattttaa gataaacctg
    31681 gcctgtaaca agtctgggtc tgtaaacatc ctgccagata gtatgatcag agtaggtggt
    31741 catgctttta taaaagtcgc tctctgataa tccaatgata acattacaca taccatcaaa
    31801 atccaaacct aactcatctg catttaacag tgcactacgt gttgtacgaa cttgcccggc
    31861 attgacaagt tttttaacct gactcaaacg tgtatgtggt gtgcgttttt ccataacccc
    31921 cgcctccctg ttactttagt tataacctaa aaggttaatt acagcaatga aaaaccacct
    31981 aaaaggttag ttagatgtac ggatataatg accacacgaa acgtattctt taaggaaagt
    32041 gattgaccat ataagaaagt ggcgcattag tagcgccagt ttgaagcagg aatttataag
    32101 ggaagctgga gtcaggcatc acgccaacgg cacataaaca tccgtttcca gtcgctgctt
    32161 tgttacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac gcattttctc
    32221 gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag
    32281 ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat tgctcacacc
    32341 ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc gaaacgcttc
    32401 cggtgcggtg gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg
    32461 agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt tgagcaactc
    32521 agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga taatgtttac
    32581 atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga acgcatcccg
    32641 ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagaat
    32701 tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacgc
    32761 cgttccagcg tttgtggtcg atatcaacat tgatagtacc gctggcatct ttgaacacgt
    32821 agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac
    32881 gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctgg aatcctccgg
    32941 cctgactttg cgttgccgat gggccagaaa aaccgccctg ctctgctgcc atcaccggtg
    33001 cgctgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct
    33061 ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa
    33121 ataaaattta gtgctgtaca gagcgcgtta caacacggtt tactggcagc aaatacggtt
    33181 atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggcgacg
    33241 gcatcaaaac gggccttagt aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc
    33301 agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat ttaaccttac
    33361 caggcatgga tggtcgcgat attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg
    33421 tactgatcct gaccgcgcgc gatgcgctgg aggaacgtgt agaagggctg cgtctgggag
    33481 ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaagctc
    33541 tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac gtcatgctcg
    33601 accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa ccaaaagaat
    33661 ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg
    33721 aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc
    33781 atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt attggctaca
    33841 cattaggtga gaaatgaaat ttacccaacg ccttagtctg cgcgtcaggc tgacgctaat
    33901 ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac
    33961 aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca agcggttaag
    34021 tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt
    34081 aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc tcgacggcag
    34141 aatggtcctt aacgatggcg ataacggaga agatattccc tatagctatc aacgggaagg
    34201 ttttgctgac gggcaactgg tgggcgataa agatcaatgg cgttttgtct ggatgacctc
    34261 acctgatggc aaatatcgca tcgttgttgg tcaggaatgg gaataccgtg aagacatggc
    34321 gctggcgatt gttgccgggc aattgatccc gtggctggtt gcactgccga ttatgttaat
    34381 catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaagctgg cgctggcgct
    34441 acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac ccagcgaagt
    34501 gcgtccactg gttgagtcgt tgaatcaact gttcgcccgc acacatgcga tgatggttcg
    34561 tgaacgacgc tttacctctg acgcagctca cgaacttcgt agcccgttaa cggcgctgaa
    34621 agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga aaaaagcact
    34681 gcttcaatta cattacggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct
    34741 atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct
    34801 cctgcaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg
    34861 actgacactc aatgtccagg gcatcaaacg caccggacaa ccgctattgc taagtttgtt
    34921 ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca cagggcagcg tggtagacgt
    34981 cacgctgaat gccgataatt tcatcgtgag ggataacggc cccggtgtga caccagaggc
    35041 actggcgcga attggcgaac gcttctatcg cccccccgga caaaccgcta ccggcagtgg
    35101 acttgggcta tcgattgtcc agcgaatcgc caagctgcat ggcatgaatg ttgagtttgg
    35161 gaatgcggaa caaggtggat ttgaggcgaa agtaagctgg taacttctct gtctatgaga
    35221 gccgttaaaa cggctctcat agattttact aatagcaaaa tataaaccgt ccccaaaaaa
    35281 gccatcaacc acaaaccaga caaataacga acgagcatac atataaccaa aactcaaaca
    35341 tttcccgcac aaagagtttt cttaagataa taataataag tggcgtcaga agaaaaaatg
    35401 ctgcaatcat tgcttgcaat atacgatc
//
LOCUS       DAZUGK010000024        34444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZUGK010000024 DAZUGK010000000
VERSION     DAZUGK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.024"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(43..528)
                     /gene="rraA"
                     /locus_tag="ACHY5W_001065"
     CDS             complement(43..528)
                     /gene="rraA"
                     /locus_tag="ACHY5W_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFS4446002.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(621..1547)
                     /gene="menA"
                     /locus_tag="ACHY5W_001066"
     CDS             complement(621..1547)
                     /gene="menA"
                     /locus_tag="ACHY5W_001066"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4446003.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(1614..2945)
                     /gene="hslU"
                     /locus_tag="ACHY5W_001067"
     CDS             complement(1614..2945)
                     /gene="hslU"
                     /locus_tag="ACHY5W_001067"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFS4446004.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(2955..3485)
                     /gene="hslV"
                     /locus_tag="ACHY5W_001068"
     CDS             complement(2955..3485)
                     /gene="hslV"
                     /locus_tag="ACHY5W_001068"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFS4446005.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(3578..4537)
                     /gene="ftsN"
                     /locus_tag="ACHY5W_001069"
     CDS             complement(3578..4537)
                     /gene="ftsN"
                     /locus_tag="ACHY5W_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFS4446006.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLTFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLATGG"
     gene            complement(4629..5654)
                     /gene="cytR"
                     /locus_tag="ACHY5W_001070"
     CDS             complement(4629..5654)
                     /gene="cytR"
                     /locus_tag="ACHY5W_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFS4446007.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(5810..8008)
                     /gene="priA"
                     /locus_tag="ACHY5W_001071"
     CDS             complement(5810..8008)
                     /gene="priA"
                     /locus_tag="ACHY5W_001071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4446008.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8211..8423
                     /gene="rpmE"
                     /locus_tag="ACHY5W_001072"
     CDS             8211..8423
                     /gene="rpmE"
                     /locus_tag="ACHY5W_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFS4446009.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(8484..9092)
                     /locus_tag="ACHY5W_001073"
     CDS             complement(8484..9092)
                     /locus_tag="ACHY5W_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFS4446010.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLNTEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDTPQLTTVAKEWPLFSW"
     gene            complement(9152..9469)
                     /gene="metJ"
                     /locus_tag="ACHY5W_001074"
     CDS             complement(9152..9469)
                     /gene="metJ"
                     /locus_tag="ACHY5W_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFS4446011.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9746..10906
                     /gene="metB"
                     /locus_tag="ACHY5W_001075"
     CDS             9746..10906
                     /gene="metB"
                     /locus_tag="ACHY5W_001075"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4446012.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSQAKRGCYRVLFVDQGDEQALRAALAEKPKLVLIESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            10909..13341
                     /gene="metL"
                     /locus_tag="ACHY5W_001076"
     CDS             10909..13341
                     /gene="metL"
                     /locus_tag="ACHY5W_001076"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFS4446013.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(13369..13614)
                     /locus_tag="ACHY5W_001077"
     CDS             complement(13369..13614)
                     /locus_tag="ACHY5W_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446014.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLIASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            13690..14580
                     /gene="metF"
                     /locus_tag="ACHY5W_001078"
     CDS             13690..14580
                     /gene="metF"
                     /locus_tag="ACHY5W_001078"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFS4446015.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPLGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            14909..17089
                     /gene="katG"
                     /locus_tag="ACHY5W_001079"
     CDS             14909..17089
                     /gene="katG"
                     /locus_tag="ACHY5W_001079"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFS4446016.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            17183..18088
                     /gene="yijE"
                     /locus_tag="ACHY5W_001080"
     CDS             17183..18088
                     /gene="yijE"
                     /locus_tag="ACHY5W_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFS4446017.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(18115..18732)
                     /locus_tag="ACHY5W_001081"
     CDS             complement(18115..18732)
                     /locus_tag="ACHY5W_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFS4446018.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     DYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(19006..20109)
                     /gene="gldA"
                     /locus_tag="ACHY5W_001082"
     CDS             complement(19006..20109)
                     /gene="gldA"
                     /locus_tag="ACHY5W_001082"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFS4446019.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(20120..20782)
                     /gene="fsa"
                     /locus_tag="ACHY5W_001083"
     CDS             complement(20120..20782)
                     /gene="fsa"
                     /locus_tag="ACHY5W_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4446020.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(20794..23295)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001084"
     CDS             complement(20794..23295)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001084"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4446021.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPRIIRARTVCSGSAGGILTPIYSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRALAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQGVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            23604..24683
                     /locus_tag="ACHY5W_001085"
     CDS             23604..24683
                     /locus_tag="ACHY5W_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFS4446022.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24698..25018
                     /locus_tag="ACHY5W_001086"
     CDS             24698..25018
                     /locus_tag="ACHY5W_001086"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS4446023.1"
                     /translation="MTKIIAVTACPSGVAHTCMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            25069..27366
                     /locus_tag="ACHY5W_001087"
     CDS             25069..27366
                     /locus_tag="ACHY5W_001087"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4446024.1"
                     /translation="MTNRISRLKTALFANTREISLERARLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRCEELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            27332..28210
                     /locus_tag="ACHY5W_001088"
     CDS             27332..28210
                     /locus_tag="ACHY5W_001088"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFS4446025.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVMENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            28212..28553
                     /locus_tag="ACHY5W_001089"
     CDS             28212..28553
                     /locus_tag="ACHY5W_001089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS4446026.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(28540..29391)
                     /locus_tag="ACHY5W_001090"
     CDS             complement(28540..29391)
                     /locus_tag="ACHY5W_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4446027.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(29606..31339)
                     /locus_tag="ACHY5W_001091"
     CDS             complement(29606..31339)
                     /locus_tag="ACHY5W_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFS4446028.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHNTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(31522..34173)
                     /gene="ppc"
                     /locus_tag="ACHY5W_001092"
     CDS             complement(31522..34173)
                     /gene="ppc"
                     /locus_tag="ACHY5W_001092"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFS4446029.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
BASE COUNT         7725 a         8958 c         9423 g         8338 t
ORIGIN      
        1 aaaaagggga atttaattcc ccttttaacg catggcagat acttattcaa tatccagcgg
       61 atcttctgaa agaataatcc cggtattgtc ggcataaaga tggtcgccgg agaagaaggt
      121 aacaccgcca aaattgacgc ggacatcgct ttcgccaatg ccttcgccag cggcaccaac
      181 cggaattgcc gccatcgcct ggatgccgat atccaactct tccaggtcat ctacctgacg
      241 caccgcgccg taaatgacca gaccttccca ttcattttgt actgccagac gcgccagttc
      301 agcatcgacc agtgcgcgac gaacagaacc accgccatcg acgacaagaa cacgaccacg
      361 gccattctgt tcgagcagat cgtacagcaa cccgttgtcc tcgaaacatt ttaccgtgat
      421 tatttgtccg ccaaacgacg cccgtccgcc aaagttggag aacagcggtt ccacgacgtt
      481 aacatcttct tgatagatgt cacaaagctc ggaagtatcg tatttcatag gcttaacatt
      541 cagttgctgc gagaattttc agtatatcgc gctatgtggt ctgttggcaa aatcatcaat
      601 tgttaattga tatttgtcag ttatgctgcc cactggctta ggaatatccc taaaacaaac
      661 agcaggttag tcagtaacgc tcccttgaca gtgcgttcca gcatcggtcg catcgccacc
      721 gggtccattt cccgcatcac ataacgggct tgcttcacca gcaacggtgc cgccagcagg
      781 aacagccagc cccacaggct atgcagcgaa aagagattaa acagcgccag acataccaga
      841 gagcccatca gcaggcaggc atgataacga cgcgcgttca cttcacctaa gcgcaccacc
      901 agcgtgtttt tgccattttc gcggtcgcta ttgatatcac gcaggttatt aatgttcagt
      961 actgccgttg ccagcaggcc gcatgcggtc gccggaagga tcagtgccgg aatcaatgta
     1021 tgagcctgta aataccagct ccccatgacg cttaaccagc caaagaaaac cagtacggaa
     1081 atatcaccca gaccgatata accataagga cgattgccca cggtgtaggt gatagcggca
     1141 ataatcgaca acccgccaag aatcaggaaa ccgacaaaat cagccagcgt atggcatgcc
     1201 actgcaacca gtgccagccc ggagagacag atgagcacga cggtaataat gagcgcccgt
     1261 ttcatctctt gctgggtaat gacccctttt tgcatgccgc gtagcggtcc gatacggtca
     1321 ggtttatcgc tgccttttac cgcatcgccg taatcattgg cgaggttaga aaggatctgt
     1381 aatagcccgg cagtaatcag tgctagcagg gcgaccagcg gatcaaaatg accttgccac
     1441 catgccagcg ccgtcccgac gataattgca gcaaaggcga gggggagggt tttaggtcgt
     1501 aaactttcca gccacgcctg agttcggcta atttgttgtt cagtcataat acgcgccaat
     1561 aaaaatgggg cctttcagcc ccatcaaaca atgatgaaaa tgattgaacg cgattatagg
     1621 ataaaacggc tcagatcttc atctgccacc aacgcatcca gatgtttgct cacataatct
     1681 gcgtcaatag tgatattttg accgcttaaa tcgctggcgt cgtaggaaat ctcttccatt
     1741 aaacgctcca gaacagtgtg taaacgacga gcaccgatgt tttcggtaga ttcgttcacc
     1801 tgccatgccg cttccgcgat gcgtttaata ccggagtcgg taaactcgat atttacgcct
     1861 tcagtcgcca tcagtgcttt gtactgcacg gtgatagagg cattcggctc ggtcagaata
     1921 cgctcgaagt cgctggtggt cagtgcctgc agttcaacgc ggattggcag acgaccttgc
     1981 agttccggga tcaggtcaga cggtttcgca atctggaacg cgccagaagc gataaacaga
     2041 atgtggtcag ttttgaccat cccgtgtttg gtggaaaccg tgcaaccttc taccagcggc
     2101 agcaggtcac gctgaacgcc ttcacgagaa acatccggac cggaagactc gccgcgctta
     2161 cagattttgt cgatttcgtc gataaacacg atcccgtgct gctcaacagc gtcgatagcg
     2221 tcttgcttca gctcttccgg gttcaccagt ttcgccgctt cttcttcaat cagcagcttc
     2281 atggcgtctt tgattttcag cttacgcgct ttttgcttct ggccgcccag gttctggaac
     2341 atggactgca gctggctggt catctcttcc atgcctggag gagccataat ttcaacgccc
     2401 atcggtgctg cggcaagatc gatctcgatt tctttgtcat caagctggcc ttcacgcagt
     2461 tttttgcgga atgcctgacg agcagcggac ggttcctgct gctgttcggt ctgtccccag
     2521 ttgtttttag ccggtgggat cagcacgtcg agaatacgtt cttctgccag ttcttcagcg
     2581 cgataacggt ttttctcgat agcctggacg cgtaccattt tcacggcggc atcggtcaga
     2641 tcgcgaataa tagaatccac ttccttaccg acgtagccca cttcggtgaa tttggtcgct
     2701 tcaactttga tgaacggcgc attcgccagc ttagccagac gacgggcgat ttcagtttta
     2761 ccgacaccgg tcgggccgat catcaggata tttttcgggg tcacttcatg gcgcagctct
     2821 tcgttgagct gcatgcgacg ccagcggtta cgcagagcaa tcgccacaga acgcttggcg
     2881 ttgtcctggc cgatgatgtg cttatccagt tcgctgacga tttcgcgtgg ggtcatttca
     2941 gacatgggag atccttacgc tttgtagctt aattcttcga tggtgtggaa atggttggta
     3001 tagatgcaaa tatcgcctgc aatatccaac gccttttcag caatttcacg agcactcagt
     3061 tcagtatttt ctaacagtgc acgcgccgca gcctgggcgt aagggccgcc ggagccgata
     3121 gcaataagat cgttttctgg ctgcaccacg tcaccgttac cggtgatgat aagcgaagcg
     3181 gtttcatccg cgactgccag cagtgcttca agtttgcgca gcatgcgatc ggtacgccag
     3241 tcttttgcca gctcaacggc ggctttgacc agatggccct gatgcatttc cagtttgcgt
     3301 tcaaacagtt cgaacagcgt aaaagcatcc gcagtaccgc ccgcaaagcc cgcgatgact
     3361 ttgtcgttgt acagacggcg gacctttttc acgttgcctt tcattacggt attgcccaac
     3421 gtggcctgac catcaccagc gatgaccaca tggccgttac ggcgtacgct tactatagtt
     3481 gtcacgagct gaccccttgg ttacgaatac agagtacaaa ccccgtacaa aagtacgggg
     3541 cataatgcaa ttatagatgg gggggatttt gagggtttca acccccggtg gcgagccgaa
     3601 tgcagtttgt atgacccgcc atcttcaacc gattgagggt gctgtctgcg ttctctttgc
     3661 ctttcaccgg gccaatgacc acacgattcc agccattgtt ggtggtaatt tttgagtcaa
     3721 agccttcgaa cgtcagctga gcacgtaccg tctctgcctg ttccgcgcct ctgaacgaac
     3781 cgcactgcac catccagcgg cgttcgtctt ttttctccgc cgtcggtttt ggcgcgtcag
     3841 cggcacgagt aaccggcgca gcttgctgcg gcttcgactg cgcggtcgtg tgcgcagggg
     3901 tttgcagcag atcctggtac ggctgctgag tagaagccgg tttagactgg cgcggttgcg
     3961 cctgtaccgg cgcggcttgc gacgtacgcg tctgctgctg ccagctttgt tcagtcgtac
     4021 gcgactgctg agccaggcgt tgctgttccg ctaattgctg cgcctgacgt tggcgctgta
     4081 gcgtttgctg acgctgttcc ggcgtctgtt cattccacgg aacctcaacc agctgcgttg
     4141 gctgctggcg catatcagcc tgcatttgtt caagaagctg acgttgttct ggtgtcagtt
     4201 gctccggcgt tttcacttca ccaccagcag aaggttctgt gggcgcacgc acacccggct
     4261 ggcgactttc cagctctttg atgtagcgcc agcgttcttc tggttttggt ggtagtccgt
     4321 ttccggtcac tttctggctt tgcagcgtct cggactcttc tttcttgtga tgcgtaatga
     4381 agtacagacc accgataaag gtcacaagaa cggcggcagc aatagcgacc atagcgggag
     4441 aaaccgcagg cagatttcgt tgctttttcc gtgaggtgct cttttttcgc cgcgaaggtg
     4501 ccggttggct gcggcgtaca taatctcgtt gtgccactat cgtttcgctg tatttattcg
     4561 ttcgtcagcc cgccatgtta cttaagcggc gggcctttga ccagtcacgg cagtcttaaa
     4621 ggtttacttt aaggtaacgc gcgtgttgat ccccggatga taagttcgca gtccattaaa
     4681 cgagagccac tgccaacgtg ttgcccctgc atttgatcaa gcaataacag catagcttcc
     4741 cggccgattt cgtaacgcgg ctgcgcgatg gttgtcagcg gcggatcaca aaattgcgtc
     4801 aggtcgatgt tatcaaaacc gattatggaa aggtcttccg ggactttcag cccctggcgt
     4861 tttgcctgag aaagtgcgcc gagcgccatc acatcgctat ggcagaagac tgcagtaggc
     4921 ggttgtggaa gatcaagcag ctgttgcatt gctttgcttc cggcttcgaa ggtgaagtcg
     4981 ccacgggcga tgtattgcgg atcaaccata atgccgcagc gacgcagcgc ctgaacgtag
     5041 ccttgcaggc ggtagtgaca cagaggcatc tcttcgggac cggctataca gccaatccgt
     5101 ttatgccctt gctcatataa ataatttact gcatcaaatg cggcggtcag attgtcgata
     5161 tgaactgtag gcagctccag ttccggtgca aattcgttcg ccatcaccat cggcggcaga
     5221 ttacgctgtt cctcaatgct ggcatcaaac ggcagccttg aacccagcaa caacatgcca
     5281 tcaatttgct tggtgatgat caaatcgata aaggtttttt cctgctgatt ttgatgcgca
     5341 cagtcgccaa tcagcaccag atatccgtga tttgccgccg taacttcgat accgcgaata
     5401 atttcgctaa agaagggatc gcagatatcc gggacaatca ccagaatggt gcgggattca
     5461 ttacgcttga cgttgcgccc cataggctgc ggtaaataac ccacttcccg ggccgctttt
     5521 tcaacccgat tacgggtggc ctgggagact ttatcgggat tcattaatgc tcgggagacg
     5581 gtcgctgtag agacttttgc cttgagggca acgtctttca tggtcgcggc agtttcctgc
     5641 ttcttcgctt tcacactcac tcctcgcctg gcacgtcagg cgtactacat ccatgtttac
     5701 ttcacatcgg caacattttt agcagatagc gcgtgaaaac ggttacagaa ttttcatgaa
     5761 aagtgtgatg aatattgaat ttttcgatcc gcctcgcatc gtgagcggtt taaccctcaa
     5821 tcggatcaac atccagcacc catttcacct tacgggaatc cggtattgta ttgatgagcg
     5881 ccagcgtacc actaatgatg tgttgcaagc gcacgcggga agggtgctgc aacaatatct
     5941 gccagcgcca gcgaccgcca cgtttaggtg ccagtgccgg aaccggaccg agaacccaca
     6001 atttgtcgtc tgccagcggg ctggagagga tcagattacg cagttgttgc aggaacagag
     6061 gcgcatgctg attgttatga tcttccgcac gcacaatcac atggctggtc cacggcggca
     6121 gctgcatcat gcgtcgctca gccagtgcct gttcggcaaa agcgtcgtag cctttataga
     6181 gcaacgtttg caatagcggg tgttccggat gatgcgtttg cagcaccact tcgccctgtt
     6241 tacccgcacg cccggcacgg cctgctacct gggtgtatag ctgggcaaaa cgctctgccg
     6301 agcgaaaatc ggcagaaaac agcgcgccgt ccacatccag taacgctacc agcgtcacat
     6361 ccggaaagtg gtgacctttc gccagcattt gcgtgccaat caagatcctc gcgccgccgc
     6421 gatgcacttc cgccagttgt tgttccagcg ctcctttgcg gctggtggta tcgcggtcga
     6481 tacgagaaat gggcacacca gggaataacg gcgcgagcgt ctgttcaagc tgttcggtgc
     6541 ccagccccac gggtaccagg tgcgtggaac cgcaggaagg gcactggcgc ggcaccgggc
     6601 gttggctgtc acagtggtga cagcgcaaat gctgttgcgc ctgatgcagc gtgtagtagt
     6661 gatcgcaacg tggacattcg gcaatccagc cgcagtcgtg gcacagcaag gcaggcgcaa
     6721 agccacggcg gttaagaaag aggataacct ggttgtcggc ctgtaaatgc tggcgcatac
     6781 gggtgattaa cgccggagcc agccccgcct gcaccttctg accttttaaa tccagcacgt
     6841 gttgaattgc cggacgcgca ttccccgccc gacgcgtcag gcgcagcagg cggtattttt
     6901 tctgctggac gttgcataac gtttccagtg cgggtgttgc agagccaaga ataatcggga
     6961 tttgctcgct gtgcgcacga taaaccgcca ggtcgcgggc atgatagcgc cagccttcct
     7021 gctgcttgta ggaactgtcg tgctcttcat caatgacaat cacaccgaga tttttaaacg
     7081 gcgtaaacag cgcggagcgg gtgccgatca caatcgccgc ctcaccattt ttcgctttca
     7141 gccacgccga aagacgctcg ctgtcgttca ggccggaatg cagaacttct acgggggcat
     7201 taaaacgttc acgaaaacgg gcgatagttt gcggtgtcag gccgatttcc ggcaccatca
     7261 ccagcgcctg tttgccctga gcgagcacgt tttccagtac gctcaaataa acctctgttt
     7321 taccggaacc tgtaacgccc gccagcagcc aggcagagaa ggtatccgcc gcgctatgaa
     7381 ttgcgccaac ggcggtggcc tgttcggtat tcaatcgcaa ccgttcacca gaaacggcat
     7441 agttcgttcg ccagtcgcta aactctggtg tttcacttgc taaatcacac agaccttttt
     7501 tgcgcagcgc ctgtaacgcg gcatcattaa attcaagcgt ggcaacctgg tcgcgccaga
     7561 ttttgccttg ccgcaatgcc gccagtgcct gttgttgctt tggcgaacgt ttcaggctgt
     7621 tcagatccac cgcctggcct tgttcagtgg caaaccagta ccacatcggc gcgttcgccg
     7681 caggccgccc ctggcgcagt aaaatcggca aggcatgaaa cagcacatcg ccaatcggat
     7741 gatgatagta atctgccgcc catagcaata atcgccagac ggagtgagta aataccggct
     7801 caacgtccag aacttcaact accgctttca gctcgttaag cggcagctca ctgacatcgc
     7861 taactgatac cacaaccccg atgcgctcct gctgtttgcc aaacggcacg cgcacgcgac
     7921 acccggcttt aaccgtcatg ccttctggca gcagatagtc aaaggtacga ggaagcggaa
     7981 cgggcaaggc aacgtgggca acgggcatag catcatcctg acttgaaatt cggtgggtta
     8041 gtatacacat tgccgtagaa gagtgcggat cagtttgcat acgctggtta atttctgtat
     8101 gatttcgcgc cttcgtacga aatgatcgta ttgaagctat acttttaaca tcgcgtggtg
     8161 tctggcgtta gggctggaag agcgacgcgg ccttaaaccg aggttttccc atgaaaaaag
     8221 atattcaccc gaaatacgaa gaaattactg ctagctgctc ttgcggtaac gtaatgaaaa
     8281 tccgctccac cgttggtcat gacctgaacc tcgacgtgtg cagcaagtgc cacccgttct
     8341 tcactggcaa acagcgtgat gttgctaccg gtggccgtgt tgaccgcttc aacaagcgtt
     8401 tcaacatccc gggcagcaaa taagaccgga tttccgaaaa aagcgccgca cggcgctttt
     8461 tttgtgcctg aattttggtc gtatcaccac gaaaacagcg gccattcttt ggcaaccgtg
     8521 gtgagttgtg gcgtatcgaa aacggcttgt ggtgagacca ccgtttcctc aagcggaata
     8581 tttttgccgt agcgttcttt gagcttcgcc tggaccagcg ggttgctgag atcaaactct
     8641 ttcaacttct gctgttcgcc cacgcgcatt cccagcgcat tctgataaac cttccacacc
     8701 acttccgaac agtactgacg atcatcgctc cacgagaagc tgaaatcgta cggtttaccg
     8761 agataacgtt tcgccgtttg cgccagtttt tgctgttgtt cggtattcag tccgccttca
     8821 acgcggcgaa caacgtattt gcccttttca ccatgggcga tccactgctt gagcggggtg
     8881 tatttcaccg ggccgaccgc ttcaaaaacg tagggctttt tgttacgcat caccagcata
     8941 ccggtgtggc tgtaatcgga atgggtcgcc agttggatcg ctttactttg cgatgagcga
     9001 gagatttgaa agatgatgtc gccagtttgc ggctgccagg caaaggcagg cacagaaacc
     9061 agcaggctga ggatcagtag cctgattttc atcgttaaac gtccttgtat gaaaaaagcg
     9121 gcaggttaac tccgcgccgc tcttttccgc tttagtattc ccacgtctcc gggttaatcc
     9181 ccatctcacg catgatctct tttgccgctt ccgggatttc gtcgctgcgc tctttacgca
     9241 gatcggcatc atccggcaaa ggttgcccgg taaaggcatg cagaaacgct tcgcacagca
     9301 gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg acgcgtgcgt tcatcggtga
     9361 ggatttttaa caccttaaga ggaatggaaa ccgtaatctt tttgacttgt tcactcttct
     9421 tgccgtgctc agcgtatggg ctgatatatt cgccgctcca ttcagccatg agatacttaa
     9481 tcctcttcgt caataaattg agatcagacc acacaattta tgtgggtact gaccgtaaac
     9541 ccgcatagtt taccgtacag gcgttaccgt gacatcgtgt aatgcacctg tcggcgtgat
     9601 aatgcatata attttaacgg ctatttggga tttgctcaat ctatacgcaa agaagtttag
     9661 atgtccagat gtattgacgt ccattaacac aatgtttact ctggtgcctg acatttcacc
     9721 gaccaagccc agggaacttc atcacatgac gcgtaaacag gccaccatcg cagtgcgtag
     9781 cgggttaaat gacgacgaac agtatggttg cgttgtccct cccatccatc tttccagcac
     9841 ctataacttt accggattta atgaaccgcg cgcgcatgat tactcgcgtc gcggcaaccc
     9901 aacgcgcgat gtggttcagc gtgcgctggc agaactggaa ggcggtgctg gtgcagtact
     9961 tactaatacc ggcatgtccg cgattcacct ggtaacgacc gtctttttga aacctggcga
    10021 tctgctggtt gcgccgcacg actgctacgg cggtagctat cgcctgttcg acagccaggc
    10081 gaaacgcggt tgctatcgcg tgttgtttgt tgatcaaggc gatgaacagg cattacgggc
    10141 agcgctggca gaaaaaccca aactggtact gatagaaagc ccaagtaatc cattgttacg
    10201 cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa gtcggggcgg tgagcgtggt
    10261 ggataacacc ttcttaagcc cggcattaca aaatccgctg gcattgggtg ccgatctggt
    10321 gttgcattca tgtacgaaat atctgaacgg tcactctgac gtggtggctg gcgtggtgat
    10381 tgctaaagac ccggacgttg tcactgaact tgcctggtgg gcaaacaata ttggcgtgac
    10441 gggcggcgcg tttgacagct atctgctgct gcgcgggttg cgaacgctgg tgccgcgtat
    10501 ggagctggcc cagcgtaacg cgcaggcgat tgtgaaatat ctgcaaaccc agccgttggt
    10561 gaaaaaactg tatcacccgt cgctgccgga aaatcagggg catgaaattg ccgcgcgtca
    10621 gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat ggcgatgagc agacgctgcg
    10681 tcgtttcctg ggcgggctgt cgttgtttac gctggcggaa tcgttagggg gagtggaaag
    10741 tttaatctct cacgccgcaa ccatgacaca tgcaggcatg gcaccagaag cgcgtgctgc
    10801 cgccgggatc tccgagacgc tgctgcgtat ctccaccggt attgaagatg gcgaagattt
    10861 aattgccgac ctggaaaatg gcttccgggc tgcaaacaag gggtaaaaat gagtgtgatt
    10921 gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg gtggcagtag tctggctgat
    10981 gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt actctcagcc tgacgatatg
    11041 atggtggttt ccgccgccgg tagcaccact aaccagttga ttaactggtt gaaactaagc
    11101 cagaccgatc gtctctctgc gcatcaggtt caacaaacgc tgcgtcgcta tcagtgcgat
    11161 ctgattagcg gcctgctacc cgccgaagaa gccgatagcc tcattagcgc ttttgtcagc
    11221 gatcttgagc gcctggcggc gctgctcgac agcggtatta acgacgcagt gtatgcggaa
    11281 gtggtgggcc acggtgaagt gtggtcggcg cgactgatgt ctgcggtact taatcaacaa
    11341 gggctgccag cggcctggct tgatgcccgc gagtttttac gcgctgaacg cgctgcgcaa
    11401 ccgcaggttg atgaagggct ttcttacccg ttgctgcaac agctactggt acaacatccg
    11461 ggtaaacgtc tggtggtgac cgggtttatc agccgtaaca acgccggtga aacggtgctg
    11521 ctggggcgta acggttccga ctattccgcg acacaaatcg gtgcgctggc gggtgtttct
    11581 cgtgtaacca tctggagcga cgtcgccggg gtatacagtg ccgatccgcg taaagtgaaa
    11641 gatgcctgtc tgctgccatt gctgcgtctg gatgaagcca gcgaactggc gcgtctggcg
    11701 gctcccgttc ttcacgcccg tactttacag ccagtatccg gtagcgaaat cgacctgcag
    11761 ctgcgctgta gctacacgcc ggatcaaggc tccacgcgca ttgaacgcgt gctggcctcc
    11821 ggtactggtg cgcgtattgt caccagccac gatgatgttt gcttgattga gtttcaggtg
    11881 cccaccagcc aggatttcaa actggcgcat aaagagatcg accagatcct gaaacgtgct
    11941 caggtgcgcc cactggcggt tggcgtgcat aacgatcgcc agttgctgca attttgctac
    12001 acctcagaag tggccgacag tgcgctgaaa atcctcgacg aagcgggatt acctggcgaa
    12061 ctgcgcctgc gtcaggggct ggcgctggta gcgatggtcg gggcgggtgt cacccgtaac
    12121 ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc agccggtcga gtttacctgg
    12181 cagtccgatg acggcatcag cctggtggcc gtactgcgca ccggcccgac cgaaagcctg
    12241 attcagggcc tgcatcagtc cgtcttccgc gcagaaaaac gcatcggcct ggtattgttc
    12301 ggcaaaggca atatcggttc ccgctggctg gaactgttcg cccgcgagca gagcacgctt
    12361 tcggcgcgta ccggctttga atttgtgctg gcaggcgtgg tggacagccg ccgcagtctg
    12421 ttgagttatg acgggctgga cgccagccgc gcgttagcct tcttcaacga tgaagcggtc
    12481 gagcaggatg aagagtcgct tttcctgtgg atgcgcgccc atccgtatga tgatttagtg
    12541 gtgctggacg ttaccgccag ccagcagctt gctgatcagt atctcgattt cgccagccac
    12601 ggtttccacg tcattagcgc caacaaactg gcaggagcga gcgacagcaa taaatatcgc
    12661 cagatccacg acgccttcga aaaaacaggg cgtcactggc tgtacaatgc caccgtcggt
    12721 gcgggcttgc cgatcaacca taccgtgcgc gatctgatcg acagcggcga tactattttg
    12781 tcgatcagtg ggatcttctc cggcacgctc tcctggctgt tcctgcaatt cgacggtagc
    12841 gtgccgttta ccgagctggt ggatcaggcg tggcagcagg gcttaaccga gcctgatccg
    12901 cgtgacgacc tctccggcaa agacgtaatg cgcaagctgg tgattctggc gcgtgaagcg
    12961 ggttacaaca ttgaaccgga ccaggtacgt gtggaatcgc tggtgcctgc tcattgcgaa
    13021 ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac ttaacgagca gatggtgcaa
    13081 cggctggaag cggcccgcga aatggggctg gtgctgcgct acgtggcgcg tttcgatgcc
    13141 aacggcaaag cgcgtgtggg cgtggaggcg gtgcgtgaag accatccgct ggcatcactg
    13201 ctgccgtgcg ataacgtctt tgccatcgaa agccgctggt atcgcgacaa cccgctggtg
    13261 atccgtgggc ctggcgcggg gcgcgacgtc accgccgggg cgattcagtc ggatatcaac
    13321 cggctggcac agttgttgta atttcagaaa tttaataatg cctggtactc atgttttcgg
    13381 gtctatggtt tctaatgaaa tatattgaat tatcatagga ttaggccgga tgaagcgttt
    13441 acgacgaatc cggcaagaag caataagtac atggttagtt aatatttgca gtccggtttg
    13501 ctttgcatac cggattttct ttttcttacc atcctgaagt tttttcatct tccctgattt
    13561 ttcctcacca tcattggtca tttttcggtt gacgcccttc ggcttttcct tcatctttac
    13621 atctggacgt ctaaacggat agatgtgcac aacacaacat ataactacaa gcgattgatg
    13681 aggtaaggta tgagcttttt tcacgccagc cagcgggatg ccctgaatca gagcctggca
    13741 gaagtccagg ggcagattaa cgtttcgttc gagtttttcc cgccgcgtac cagtgaaatg
    13801 gagcagaccc tgtggaactc catcgatcgc cttagcagcc tgaaaccaaa gtttgtatcg
    13861 gtgacctatg gcgcgaactc cggcgagcgt gaccgtacgc acagcattat taaaggcatt
    13921 aaagatcgca ccggtctgga agcggcaccg catcttactt gcattgatgc gacgcccgac
    13981 gagttgcgca ccattgcacg cgactactgg aataacggta ttcgtcatat cgtggcgctg
    14041 cgtggcgatc tgccgctggg aagtggtaag ccagaaatgt atgcttctga cctggtgacg
    14101 ctgttaaaag aagtggcaga tttcgatatc tccgtggcgg cgtatccgga agttcaccca
    14161 gaagcaaaaa gcgctcaggc ggatttgctt aacctgaaac gcaaagtcga tgccggagcc
    14221 aaccgcgcga ttactcagtt cttctttgat gtcgaaagct acctgcgttt tcgtgaccgc
    14281 tgtgtgtcgg cgggcattga tgtggaaatt attccgggaa ttttgccggt atctaacttt
    14341 aaacaggcga agaaatttgc cgatatgacc aacgtgcgta ttccggcgtg gatggcgcaa
    14401 atgttcgacg gcctggatga tgatgccgaa acccgcaaac tggttggcgc gaatattgcc
    14461 atggatatgg tgaagatttt aagccgtgaa ggagtgaaag atttccactt ctatacgctt
    14521 aaccgtgctg aaatgagtta cgcgatttgc catacgctgg gggttcgacc tggtttataa
    14581 atagtgtggc ttttgtgaaa atcacacagt gatcacaaat tttaaacaga gcacaaaatg
    14641 ctgcctcgaa atgagggcgg gaaaataagg ttatcagcct tgttttctcc ctcattactt
    14701 gaaggatatg aagctaaaac ccttttttat aaagcatttg tccgaattcg gacataatca
    14761 aaaaagctta attacgatca atttgatcta catctcttta accaacaata tgtaagatct
    14821 caactatcgc atccgtggat taattcaatt ataacttctc tctaacgctg tgtatcgtaa
    14881 cggtaacact gtagagggga gcacattgat gagcacgtca gacgatatcc ataacacgac
    14941 agccactggc aaatgcccgt tccatcaggg cggtcacgac cagagcgcgg gggcgggcac
    15001 aaccactcgc gactggtggc caaatcaact ccgtgttgac ctgttaaacc aacattctaa
    15061 tcgttctaac ccgctgggtg aggactttga ctaccgcaaa gaattcagca aattagatta
    15121 ctacggcctg aaaaaagatc tgaaagccct gctgacagaa tctcaaccgt ggtggccagc
    15181 cgactggggc agctacgccg gtctgtttat tcgtatggcc tggcacggtg cgggtactta
    15241 ccgttcaatc gatgggcgcg gcggcgcggg tcgtggtcag caacgttttg caccgctgaa
    15301 ctcctggccg gataacgtaa gcctcgataa agcgcgtcgc ctgttgtggc caatcaaaca
    15361 gaaatatggt cagaaaatct cctgggccga cctgtttatc ctcgcgggta acgtggcgct
    15421 ggaaaactcc ggcttccgta ccttcggttt tggtgccggt cgtgaagacg tctgggaacc
    15481 ggatctggat gttaactggg gtgatgaaaa agcctggctg actcaccgtc atccggaagc
    15541 gctggcgaaa gcaccgctgg gcgcaaccga gatgggcctg atttacgtta acccggaagg
    15601 ccctgatcac agcggcgaac cgctttctgc ggcagcagct atccgcgcga ccttcggcaa
    15661 catgggtatg aacgacgaag aaaccgtggc gctgattgcg ggtggtcata cgctgggtaa
    15721 aacccacggt gctggtccga catcaaatgt aggtcctgat ccagaagctg caccgattga
    15781 agaacaaggt ttaggctggg cgagcactta cggcagcggc gttggcgcag atgccattac
    15841 ctctggtctg gaagtagtct ggacccagac gccgacccag tggagcaact atttcttcga
    15901 gaatctgttc aagtatgagt gggtacagac ccgcagtccg gctggcgcaa tccagttcga
    15961 agcggtagac gcaccggaaa ttatcccgga tccgtttgat ccgtcgaaga aacgtaaacc
    16021 gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct gagttcgaga agatctctcg
    16081 tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt gcccgtgcct ggttcaaact
    16141 gacgcacagg gatatggggc cgaaatctcg ttacatcggg ccggaagtac cgaaagaaga
    16201 tctgatctgg caagatccgc tgccgcagcc gatctacaac ccgaccgagc aggacattat
    16261 cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt agtgagctgg tatcggtggc
    16321 ctgggcttct gcttctacct tccgtggtgg cgacaaacgc ggtggtgcca acggtgcgcg
    16381 tctggcatta atgccgcagc gcgactggga tgtgaacgca gcagccgttc gtgctctgcc
    16441 agttctggag aaaattcaga aagagtctgg taaagcctcg ctggcggata tcatcgtgct
    16501 ggcaggtgtg gttggcgttg agaaagccgc aagcgctgca ggtttgagca ttcatgtacc
    16561 gtttgcgccg ggtcgcgttg atgcgcgtca ggatcagact gacattgaaa tgtttgagct
    16621 gcttgagcca attgctgacg gtttccgtaa ctatcgcgct cgtctggacg tttccaccac
    16681 cgagtcactg ctgatcgaca aagcccagca actgaccctg accgcgccgg aaatgaccgc
    16741 gctggtaggc gggatgcgtg tactgggtgc caacttcgat ggcagcaaaa acggcgtctt
    16801 cactgaccgc gttggcgtat tgagcaatga cttcttcgtg aacttgctgg atatgcgtta
    16861 cgagtggaaa gcgaccgacg aatcgaaaga gctgtttgaa ggccgtgacc gcgaaaccgg
    16921 cgaagtgaaa tacactgcca gccgtgcgga tctggtattt ggttctaact ctgtcctgcg
    16981 tgcggtagcg gaagtttacg ccagcagcga tgcccacgag aagtttgtta aagacttcgt
    17041 ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac ctgctgtaat ctgaccttct
    17101 tcagcgactg tctttcaggc agtcgctgaa gtttctttac cggcgtatag tgtccacagg
    17161 aaaactacac actggatctc tcatgtctgc cgcaggaaag agcaacccac tggcaatcag
    17221 tggcctggtt gtgctcacac ttatctggag ttatagctgg attttcatga agcaagtcac
    17281 cagctacatc ggtgccttcg actttaccgc cttacgctgc attttcggcg ctctcgtttt
    17341 attcatcgtc cttttattac gtggtcgcgg aatgcgcccg acgccgttta aatacacctt
    17401 agccattgcc ctgttacaaa cctgcgggat ggttggcctg gcacagtggg cgctggttag
    17461 cggcggagcg gggaaagtgg cgatcctgag ctataccatg ccgttctggg tggtgatttt
    17521 cgccgcgttg tttctcggtg aacgcctgcg acgtgggcaa tatttcgcga tactgattgc
    17581 tgctttcggc ttatttttgg tgttgcagcc gtggcaactc gatttctctt cgatgaaaag
    17641 tgccatgctg gcaatcctct ccggtgttag ttggggggcg agcgcgattg ttgctaaacg
    17701 cctatacgcc cgtcatccgc gcgtggattt attgtcgtta acatcctggc agatgctcta
    17761 tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg caacgtgaaa ttgactggca
    17821 gcccaccgtg ttctgggcgc tggcctacag tgcgattctg gcgacggcgc tggcgtggag
    17881 cttatggctg tttgtgctga aaaacttgcc tgccagtatt gccagcttaa gcacactggc
    17941 cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc ggtgagaatc cgggggccgt
    18001 tgaaggtagc ggtattgtgc tgattgtgat ggcactggcg ctggtgagcc gtaagaaaaa
    18061 agaagccgtt agtgtaaaaa ggatctgaat tttttcttca tgtggggcga tcgcttattt
    18121 aacaaaataa cgataatgcc ccaccatgcg ccagctaaac agcacatctt cttcctgcgc
    18181 acctgcgcca atgttatgta tcaccagcgg cgtaccgtcg cgggcgaagc catctgaaac
    18241 caccccaata tgtgccagcc cgttatctaa tcgccaggag acaatatcgc ccgcttgata
    18301 gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg cgggtaaacc aggtttccag
    18361 attaggtacc cgacggtgat cgatgttgct gtccgggcgc tttaactgcc acttttgcgg
    18421 ataatcggca aaattcttcg ccatatcttc gtgaaccagt ttctgcaaat cgaccttctg
    18481 gctgcgcaat gcacggatca ccacatcgga acatacaccg cgttcttgcg gaacatcacc
    18541 gccaggatag gtaagctgca tatacgccgg atcgtaaaat agcgtgctgc caatttgctg
    18601 tctggcaccg tctgcgatgg caaggttggt attggcctgg atttgtacca cggttggtgg
    18661 aacggcggga gattttaagg agtggctggt aaatgccgtt agcaggctga gcagcgccag
    18721 tgaagctttc atctggttcg tccgtgaaaa taagagtcac agattcgacc ttccgggcta
    18781 acgattgttc tgtggagaaa atgtccagaa gcggaaaaaa aattcctgaa caacgtggga
    18841 gtttcagatt actggcacag agtgccggat gcggcgtgaa cgccttatct ggcctaaaaa
    18901 gcacgcaaat tcaatatatt gcagagattg cgtaggcctg ataagcgtag cgcatcaggc
    18961 aattttgcgt tcaaactccc ggacaagccg ggagtttgga gtaggttatt cccactcttg
    19021 caggaaacgt tgtccatact ggtcagctac cagcagtgcg gcgtaaacct gatctggcgt
    19081 cgcgccgcca ggcatgttgt gaatggtttc accttctgca catgccgctt ctgccacaat
    19141 tcgcattttc gccgggacat cttctttaat atccagttga gcgagagtta ttggcaaacc
    19201 taccgcatgg ctaagggcag ctacggtttc gatttcctcc accggcgcat tttccagaac
    19261 cagctgcgtc agcgtaccga atgccacttt ttcaccgtga taatagtgat gcgcgtccgg
    19321 gatagcggtc aggccgttat gcactgcgtg cgccgcagcc agaccaccac tttcaaaacc
    19381 aacaccgctc aaataggtgt tcgcttcaat cacgcgctcc agcgccggag tcactacatg
    19441 ctgttcggca gcaagcatcg ctttttcgcc ttcttccagc agggtgttgt agcacagttc
    19501 agccagtgcc agcgcagcct gggtgcactt gccgcccgcc atggtggtcg cgccgctacg
    19561 agagcaggca cgcgcttcaa accaggttgc cagcgcatcg ccgatacccg ccgctaacag
    19621 acgtgcaggt gcgccagcga cgattttggt gtcgacaatg accatattcg ggttatttgg
    19681 caacagcaga tagcggtcaa actcaccctc atcggtgtag ataacagaca atgcgctgca
    19741 cggtgcgtcg gtagaggcga tagtcggtgc gatcgctacc ggaacaccca tgaaatgtgc
    19801 cagtgctttg gcagtatcga gagtttttcc gccaccgata ccgagaattg cgccacactg
    19861 cgcagtctcc gcaatgccac gcaggcggtc gatctcattt tgcgaacatt caccgccaaa
    19921 cggcgcaatt tctactacca gtccagcatc tttaaagctt ttctcgacag tggattgagc
    19981 aaaacctaaa acaaatttgt cacctaccac taaccagcgt tctgccagcg gcttcaggta
    20041 ttcgcccaga cgattaatca catcagcgcc ctggatgtat ttacccggtg attgaataat
    20101 gcggtccata attgctcctt tagagatgag tagtgccaaa tgcggcattc cagtcgtgtt
    20161 cgaacttctc tatagctgac tctaccgcag gggtgttgag catttgttgc gctacatcta
    20221 agggcagggt gatggattca catcctgcca gtaaacagtc cagcgcctga cgcggcgttt
    20281 taaagctggc cgccagcacc atgctttctg gtgcgtgcat ttctaacagc gcttgcagct
    20341 cctgaaccgt acgaatgccg tccccgccct gggcatctac gcggttaaca tacggagcaa
    20401 cgtattttgc ccctgccagt gcggctaata acccttgtgc ggcgctatat acagcagtgc
    20461 caagtgtagt aataccctct tttttcagta tcttaattgc tgccagacct tcggaagtca
    20521 ccgggatttt caccacaatg cccggaatgg cgtcgcgcag acgcttcgct tcttccacca
    20581 tcccctgcgc gtcgcggctc atggtctgag caaacagaat gccctcatca ccaatcgctt
    20641 tttgcagacg cggcagcact tcccagatgg actccttgct ggcagcgata atgctcgggt
    20701 tagttgtcac cccggcaatc gggaatatgc gtgccagacg ttcgacttct gcgacgttag
    20761 cggtgtccag atacagttcc atgatgtttc cttttacagt tccagttcat gttgcagcag
    20821 gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg ttacggaatt cttcgtgcat
    20881 gatgcgacga gcgaggcgcg agaaaatgcg catatgctga tcgcccgcag cgtgtttgtt
    20941 cagggttaac ataatgatga attgcgcttc atcatcgccc cagcgcaccg gcgcttgcag
    21001 acgcgctacg ctgatggtgg attgctcaat gtgttctgat ttgctgtgtg gaatggcaaa
    21061 actaaagccc agaccggtag agaaaacggc ctcgcgcgcc cacaagtcgg cttccagttt
    21121 acgcggatag cggcagcgcc ctgccagcag caggttatcg gtcatgcctt tgagcacttc
    21181 ttctttgctg cgccagtcgc tttccagcgt gatgcactcg gcggtgacca gtggtgcgtc
    21241 ctgttgggtc atgcggaatt gcgccagcag atgttccact tccagcgaag tacggcaggc
    21301 cattgcctgg ttgagcaact ggcggcactc acggctatca agttgcgcca tccgcgcttt
    21361 cgccgccgga attgatggtg cgctcatgct cagctcatcc agccctaaac cgaccagcaa
    21421 cggcagcacg gaaccttttg cccccagctc accgcacaga ccaatccatt tgccctggcg
    21481 atgcaccgcc tgcacggcgt aatcgagcgc ccgcaagaat gccggattca ggctgttgta
    21541 gtgacgagta accttagcat tatcgcgatc caccgccagc agatattgcg tcaggtcatt
    21601 actaccaata ctaaagaaat caatctcttc gcagcattga tcgatgatga acatcaccga
    21661 cggcacttcc agcataatac cgagctggat cttctcatca aacggaatgt gttcgttacg
    21721 cagttgctgt ttggcttccg ccagtttttc tttcacccat aagatctctt ccattgaaga
    21781 gatcatcggg atcatgattt tcaggctgcc gtgagcggag gcgcgcagga tcgaccgtag
    21841 ttgtgtggtg aacagcgagg cgtattcttc ataaatacgc acagcgcgat aaccgaggaa
    21901 cgggttagct tctgccggaa tgttcagata atcaacgggt ttgtcgccgc caatgtccat
    21961 agtgcgcaca ataatgctgc gtccgttggc ggactccagc gcctgacaaa aaatgttgta
    22021 cagctcgctt tcgcccggtg cactggtgcg atccatatag agcatttcag tacggaacaa
    22081 accaacgcct tccgcaccat tgccgaatgc ggcctgcgct tccacggagt gagcgatgtt
    22141 agcggcaatt tcaatgcgga taccgtcagc ggtacgggct tgttgggtca gccagacacc
    22201 ctgttgctca cgaagggcgt cctgtacgcg ggcttcttgc tgataataac gagctacggc
    22261 ttcccctggc tcaaccacaa tcgccccggc gttgccgtcg atataaatcg tttgatgctg
    22321 ccacggagta agggcatcaa tatctacacc aaccagtgtc ggaatgttga acgaacgggc
    22381 aagaattacg gtatgtgagg tggtgccgcc acttttcagt aacagacctt tgaggtgatt
    22441 tttatccagt tcgaggaact ggctgggggt cagttcatca gccatacaaa tggcgggctg
    22501 cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag atttgctgga gtaactggaa
    22561 gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag ctgctgctgg aacgggaaaa
    22621 ctcttcacaa aagtgattcg cactggcgac aatcgcttcg gcgcagctta atccggcgct
    22681 gacgcctgcc agtaaatgtt cgtgcaggga agtatcgcca gccagggctc ggtgcgcttc
    22741 cagaatcgcg ctggtggcac cgtcgctatc cagcagacga aactcaatgt ttttcagtac
    22801 cagcgtcagg ccgttttcca gtgcggattg ctcggcgtca acgtctttgg cggcgggaag
    22861 attacccagc gcgttgagat ctaaagaata gatcggcgtc agaatgccgc cagcgctacc
    22921 gctgcacacg gtgcgggcgc gaataatgcg cggattcaaa ttagtgagtg aaaccggcag
    22981 cggctccagc tcgtcagatt ttacttccgc cagcggtgcg tcgcagtggg ggaattcatc
    23041 gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg tcggccccgg aaatcagtaa
    23101 ctggcagtta tcgcccgcca gcgtgtcggt gccaatcagc gcaagggcgc ttttggcgtt
    23161 gcccttgcgg tcagtgcgca ggttatgcca ctcaatttgt gatgaaaaag tattacacag
    23221 cgtttcaacg tggcttgccg gacgtgcatg tacgccgtta ggtagctcac aaataaattc
    23281 cacaatcagg gccattgcct ctctcccggt acggttttct gtttacagga taagagcgca
    23341 cggcaacggc ctgccatgtg acaaatctgc caaaagctgg acaaatgtaa tgtaaccgtc
    23401 aatttgcgac gcgtctcaca agacgccgtt ttgcggcatg cttccggatt atcgcaagtt
    23461 atgaggcgga tcgcattttt gtactgatat tacaaaagtc cagtaaatgg cctttttatc
    23521 cactgtttgc tccgcctgcg attgcctatt gtttcctcca tcaaaaatat ggacatgggc
    23581 tttcgcaagc ccgggagcag cttatgaatg agttggtgca gatcctgaaa aatacccgtc
    23641 agcatttaat gacgggcgtt tcacacatga ttcccttcgt ggtatcgggc ggtattttgc
    23701 tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc ggatgccgta gccgatccga
    23761 atctgaaaaa actgtttgat atcggcgttg caggtttgac gctgatggtg cctttcctcg
    23821 ccgcttacat tggttactcc attgcagagc gttctgcgct ggccccgtgc gctatcggtg
    23881 cctgggttgg taacagcttt ggtgcgggct tctttggtgc gctgatcgcc gggattattg
    23941 gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca taaagttctg cgctcggtga
    24001 tgcccatctt catcattccg atcgtcggca cactgattac cgcaggcatc atgatgtggg
    24061 gtctgggcga gcctgtaggg gcgttgacca acagcctgac tcagtggctt caggggatgc
    24121 agcagggcag cattgttatg ctggcggtga tcatgggtct gatgctggcg ttcgatatgg
    24181 gcggtccggt taacaaagtg gcctatgcct tcatgctgat ttgcgttgct cagggtgttt
    24241 ataccgtggt ggccattgct gccgtgggta tttgtatccc gccgctgggg atggggctgg
    24301 cgacgctgat tggtcgtaaa aatttctccg cagaagagcg cgaaaccggt aaagcggcgc
    24361 tggtgatggg gtgcgttggg gttactgaag gggcgattcc tttcgccgca gccgatccgt
    24421 tacgtgtaat tccttccatc atggttggtt cagtttgtgg tgcagtaact gcggcgctgg
    24481 tcggtgcgca gtgctatgca ggctggggtg gtctgattgt gctgccggtg gttgaaggta
    24541 agttgggtta tatcgcagca gtggctgtcg gggcggttgt gacggcagtc tgcgtgaacg
    24601 tgctgaaaag tctggcgcgt aaaaatggat cttctactga tgaaaaagaa gacgacctgg
    24661 atttggattt tgaaattaat taattgagga acgagccatg acgaaaatta ttgcagtaac
    24721 cgcgtgtcca tctggtgttg cccatacttg tatggcggca gaggcgctgg aaagtgccgc
    24781 gaaagcgaaa ggctgggaag tgaaagtaga aacccagggc tcgattggtc tggaaaacga
    24841 actgactgcg gaagacgtgg cgagcgccga tatggtgatt ctgactaaag atatcggcat
    24901 caagtttgaa gagcgttttg ctggcaagac catcgtgcgc gtcaacatca gcgatgcggt
    24961 aaaacgtgct gatgccatta tgagcaaaat tgaagctcac ctggcgcaaa ccgcttaagt
    25021 tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg agttcctcat gacgaatcgt
    25081 atctctcgcc tcaaaactgc actgtttgcc aatacccgtg aaatctcgct ggagcgtgcg
    25141 cggctttata ccgccagcca tcggcaaacc gaaggcgaac cggtgatatt gcgccgggcg
    25201 aaagcaacag cgtatatcct tgaacatgtt gaaatttcga ttcgtgatga agaactgatt
    25261 gccggtaacc gcacggtgaa accacgcgcc gggattatgt cgccggaaat ggacccttac
    25321 tggctgctga aagagctgga tcaattcccg acgcgtccgc aggaccgctt tgctatcagc
    25381 gaagaagata aacgtatcta ccgcgaagag ttgttcccgt actgggaaaa acgttcgatg
    25441 aaagatttca tcaacgggca gatgacggat gaagtaaaag ccgcgaccag cacgcagatt
    25501 ttcagcatca accagacgga taaaggccag gggcacatta ttattgatta cccgcgcctg
    25561 ctgaatcacg ggctggggga gctggtggca cagatgcagc aacattgtca gcaacagccg
    25621 gagaatcact tttatcaggc agcgctgtta ctgctggaag cctcgcagaa acacattttg
    25681 cgttacgccg aactggcgga aacgatggcg gcaaactgca cagatgccca gcgttgcgaa
    25741 gaactgctga ctattgcgga gatctcccgc cataacgccg aacataagcc acagacgttc
    25801 tggcaggcgt gccagttatt ctggtacatg aacatcattc tgcaatacga atccaacgcc
    25861 agttcgctgt ctttagggcg cttcgaccag tatatgttgc cgttctatca ggcatcttta
    25921 acccagggcg aagatccggc gttcctgaaa gaactgctcg aatctttatg ggtgaaatgc
    25981 aacgacattg tgctgttgcg ctccaccagc agcgcgcgtt atttcgcagg tttcccgact
    26041 ggctataccg cactgctcgg cgggttaacc gagaacggac gtagcgcggt gaacgtgctt
    26101 tcgttcctct gcctcgatgc ctatcaaagc gtacaattac cgcaaccgaa cctaggggtg
    26161 cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa ccgcagaaac cattcgcctc
    26221 ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg tgccagcgtt cctcaaccgt
    26281 ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg gctgtgtgga attatctatt
    26341 cccggcagaa cctacggctt gcatgacatc gcgatgttta atctgctgaa agtgatggaa
    26401 atctgcctgc atgaaaatga aggcaatgct gcgctgactt atgaaggttt actggagcag
    26461 atccgcgcca agatcagcca ctacatcacc ctgatggttg agggcagcaa tatttgtgat
    26521 atcggccatc gcgactgggc acctgtaccg ctgctctcgt cttttatcag cgattgtctg
    26581 gaaaaaggtc gcgatattac cgatggcggc gcgcgttata acttctccgg cgtacaggga
    26641 attggtatcg ccaacctgag cgattctctc catgcgttga aagggatggt ttttgatcaa
    26701 cagcgtttaa gttttgacga attgctgtcg gtattaaaag ccaactttgc aacgccagaa
    26761 ggcgaaaaag tccgcgctcg cttaattaac cgctttgaga aatacggtaa cgatatcgac
    26821 gaggtggata acattagcgc cgaactgttg cgccactact gcaaagaagt ggaaaaatac
    26881 cagaacccgc gcggcggtta cttcacgccg ggatcgtata ccgtttctgc tcacgtcccg
    26941 ttgggatcgg tggttggcgc gacgccagac ggtcgttttg ccggagaaca gctggcagac
    27001 ggcggcttgt cacccatgct gggccaggac gcacaagggc caacagcggt actgaagtca
    27061 gtcagtaagc tcgataacac gctgctgtct aacggtacgt tgctgaacgt gaaattcact
    27121 ccggcgaccc tggaaggtga agcgggatta cgcaaactgg ccgacttctt acgggcgttt
    27181 acccagctta agttgcagca tattcagttt aacgtggtga acgccgacac gttgcgggaa
    27241 gcacaacagc gcccacaaga ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc
    27301 ttctttgtcg aactgtcgaa ggagatccag gatgacatca tccgccggac agcgcatcag
    27361 ctgtaacgtt gtggaaacgc gccgcaatga tgtggcgcgt attttcaaca ttcagcgtta
    27421 ttcactgaat gacggcgagg gcattcgtac ggtggtcttt tttaaaggct gtccgcatct
    27481 ttgcccgtgg tgtgctaatc cggagtcgat ctccggcaaa atccagacgg tacgcagaga
    27541 ggcgaaatgt ctgcactgtg cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc
    27601 gtttgaacgg attggtcgcg atatcagcct tgacgcgctg gaacgggaag tgatgaaaga
    27661 tgacattttc tttcgcacgt ccggcggcgg cgtcacgctt tctggcggcg aagtgttaat
    27721 gcaggcggag tttgctaccc gttttttaca gcgactgcgg ctgtggggtg tgtcatgcgc
    27781 cattgaaact gccggagacg caccagccag caagctgtta ccactggcga aattgtgcga
    27841 tgaagtgttg ttcgatttaa aaatcatgga tgcgactcag gcgcgggatg tggtgaagat
    27901 gaacctgcca cgcgtgatgg agaatctgcg tttgctggtg agtgagggtg tcaacgtgat
    27961 cccgcgttta ccgctgatcc ctggtttcac gctcagccgg gagaatatgc agcaggcgct
    28021 ggatgtactg atcccgctga aaatcaggca gatccatctg ttaccgtttc atcagtacgg
    28081 cgaaccgaaa taccgcctgc tggggaaaac atggtcgatg aaagaggtgc ctgcgccgtc
    28141 gtcagccgat gtgacaacga tgcgcgaaat ggcagaacgg gccggatttc aggttaccgt
    28201 gggaggttaa aatggcatac ctggtggcag taaccgcctg cgtcagtggc gtggcgcata
    28261 cttatatggc ggcggaacgg ctggaaaagt tgtgcctgtt agagaagtgg ggagtcagca
    28321 ttgaaactca gggcgcgctg ggaacggaga atcgtttagc ggacgaggat atccgtcggg
    28381 cggatgttgc tctgttgatt acggatatcg agcttgccgg tgccgagcga tttgaacatt
    28441 gccgctatgt gcaatgcagc atctacgcat tcctgcgtga gccgcagcgg gtaatgagcg
    28501 cggtgcgcaa agtgctttct gcgccgcagc aaacccatct tattctggag tagtcggttt
    28561 ttctgtcagt tggctgtggt actgccggcg atattccgac ggcgagcgtt cggtgttttt
    28621 acgaaacaga cggcagaagt agttgctgtc gacaaagccg caggcgtgcg ccacttcttt
    28681 taccttcagg tcatagcctt tcaataacgt cttagcgtgc tccagtcgcg tgtgattcag
    28741 gtattcgtta aaaccaatgg cccccgtttt ttgaaacagg tgcgagagat agtttggtga
    28801 aatatagaat gcctgtgcga cagattcgcg ggtaagcgcg gaggcatagc gttcgtcgat
    28861 ataatcgcga atagcttcga atagtgcctg gctgcgtgag gcggtctgga tttggctgcc
    28921 aagcaaatcg cggcagtggc taagcaggct ggcgacgata agccttgccg tttgctgctc
    28981 ctgcggctgc atttgcattt cattgagcgt ttgtagcaga aaagaaccta tgcgtgggcc
    29041 gcgtcgggcg acgtgttgct tcgccagatt ttgatattgt ttgccatccc attgcacgac
    29101 gctgaagccg agttgctgtt tgccaaacag cacgctaaag gtagtagcgg gggcttgcca
    29161 ttgtggaaaa ttccagccac cagcggcaac gtacagcaca tcgccgggaa ctaacgttgc
    29221 tccagcgccg gtatcaacaa actcaccttc cagcacaatt tccagccgtg gaaaatcgac
    29281 ctgatacgca agatcgggaa ccgggccgtt actgctggca aagtaaatct ggcgcaggga
    29341 cagcgggccg ttgatcaggc gggagagcag atagctgacg tcgtgataca tgttattccc
    29401 ttaggtatgt agacgggaaa acagtaactg gcattattaa aaaaggccac ctgaaaagtg
    29461 tggcctgagg ggagttcgat gtaaacattg cggcagttgt attgccggat gtggcgtaaa
    29521 cgccttatcc agtctaccgc cgcgatctgt aggccggata agacgcgtca agcgtcgcat
    29581 ccggcaaata gcgcctggct gataattact gattacctac ctggtcgcca tacggcagcg
    29641 tgtcgtaatc gatcagcgcg tttttcttgt acgggttacc aatccagcgg gtagtttctt
    29701 tgaactgcgg attcaccact gaacgggttg gatcgtaacc gtcgtaagat aagcccgcta
    29761 aatctgacca ggtgtggatc agttccgcca ggctgtattt acgatcaaca tcctgtgaga
    29821 aatcacgcgg atgagtcgct tgccattttt ccgacgtcca cagcaggaac ggaatggtgt
    29881 acatatgacg cgtcgggttg tcctcattac gcccctgggt gttatgaggc ggcgtgtcgt
    29941 aaacctcttc accgtggtca gagaaataaa ccaggaaacc gttcgggtct gctgctttaa
    30001 agtctttaat caggctggca accacatgat cgttatacag gttagcgttg tcataatcgt
    30061 tatatgattc cagctcttct gcgcttaatc ctggcggaac atgatcggta ttgccatcaa
    30121 acttgccctg attttccggg tagcggtatt tgtatttgat atgcgtaccc agcagatgaa
    30181 cgatgatcag tttcttcggc gcagggtcct tcagcacttc ctggaacggc ttcagcacgt
    30241 tagtgtcgta ttcacgcgca ctctgcgtac gttgctggtt catgtagtac tgcttgtcgg
    30301 tctggcgcga aaataccgtc agcatggtat tgcgggcggt catcgtctgc tggttggtga
    30361 tccagaaggt tttataaccc gcctgtttca tcatgttcat cagcgacggc tgcgtcagat
    30421 acagatccgg gttcttttca ttggcaaagg tcagcgcctg ttgcaggatt tcaatggtgt
    30481 acggacgaga cgtaactacg ttattaaaca cggttagatt cggatcggtt ttatgcagcg
    30541 catccagctc cggcgtggtt tcacgcggat aaccgtacag actcatgcgt ccacgctggg
    30601 ttgactcgcc aatcaccagc actaaggtac gcggttcgtt acccgattca tctttgaaat
    30661 tagccagcgg cggcaaggca ttattttcat tcagtagctt tgttagcgag tttagttgct
    30721 gacgatactg ataatagccg gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg
    30781 ccaggttatc caacgttttc tcaaacggct tgtttttgat aaacgtattc atggcgatcg
    30841 gatgcagaat caagccataa agcagggcaa aagagacaac ataacgccac ggctttggaa
    30901 tatagaccgg gcgcagacgt gtccacagca gaactgccac cgccgtatag gccagcgcga
    30961 taagcacaat tttcaggctg aaatactggc ttaaatactc gctggcttcg ttggtgttgg
    31021 tttcgaacat cacaaacaga acgctctgcg agaactcctg accgtagatg acgtagtagc
    31081 acagcgccgc cagagaggct gcccatagca ccacgccgat cactgcggca ataattttga
    31141 tccgcttcgg aaagaggaat accggaatta accacagcga gctgaataac agcgagtcgc
    31201 gaatgccgtt agtgccacta taaccactga tgtaaataat ggcctgtagc agagtagaga
    31261 aaaaccaaaa gtagagcagt gcccaaccca gggctttcca gctaaaaaga ggtttagcct
    31321 ggacttctgt ggaatgcata gtaagaacct gtcttgaaaa aaatatcgcc gaatgtaccg
    31381 acaattcctt aaggatatct gaagggatat ccagaatttg aataaaatgc agacagaaat
    31441 atattgaaaa cgagggtgtt agaacagaag tatttcagaa aaccctcgcg caaaagcacg
    31501 agggtttgca ggagaggaag attagccggt attacgcata cctgccgcaa tcccggcaat
    31561 agtgaccatt aacgcctgct cgacgcgagg atccggttcc tggccttctt tttctgcctg
    31621 gcgggagcgg tgcagcaact cggcttgcaa tacgttcagc gggtcggtgt aaatattccg
    31681 tagctgaata gactctgcaa tccacggcag atcggccatc agatgggaat cgttggcaat
    31741 cgccagcacc actttgatgt cttcttcttg cagattgcgt aactctttac ctaacggcca
    31801 cagtgctttg tctaccagac gttgatcata gtattccgcc agccacaggt ctgctttggc
    31861 gaagaccatc tccagcatgc cgagacgcgt cgagaagaat ggccaatcgc ggcacatagc
    31921 ttccagctcg ctctgtttgc catcttcgac cactttttgc agcgccgtac ctgcacccag
    31981 ccaggcgggg agcatcagac ggttttgcgt ccaggcaaaa atccacggaa tggcgcgcag
    32041 cgactcgacg ccgccggtcg ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc
    32101 cagttcttgt tccggtgtag cggagcggaa gtaaggcaca aaatctttgt tttcacgtac
    32161 gtagccacgg tagagatcgc aggagatgac tgatagttca tccataatgc gacgccagct
    32221 ctctttcggc tcaggtggtg gcagcaggtt ggcttccaga atcgccccgg tgtaaagcga
    32281 caggctgctg acggtgattt ctggcagacc atatttaaag cggatcatct cgccctgttc
    32341 agtcacgcgc aggccacctt tcaggcttcc tggcggttgt gacagcagcg ccgcatgagc
    32401 aggtgcgccg ccgcgaccaa tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc
    32461 cgctttttcg caggttttga ttaatgcatc ctgtgcctga tattgcgccc aggacgctgc
    32521 catcacgccc gcatcttttg ctgagtcgga atagccaatc atcaccatct gtttgccctg
    32581 aatcaggccg cgataccagt cgatattcag caactgagtc atgacatcgt tggcgttgtt
    32641 caggtcatcg agagtttcaa acagcggagc aaccggcatc gcaaacccga tacccgcttc
    32701 tttcagcagc aggtggacag ccagcacgtc ggacggcgtt ttcgccatcg agatcacgta
    32761 ggcggcaatg gagccttgcg gtgcttcggc aatcacctgg caggtatcga gcacttcgcg
    32821 cgtttcggcg cttggttgcc agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg
    32881 gatcaggaac gcctgtttgt cggcctctga ccagctttcg tagtcgccga taccgaggta
    32941 gcgggtcagc tcgcccagcg cttcggtatg gcgggtgctc tcctgacgga tatcaatacg
    33001 gaccagcggt acgccgaaac atttcacgcg gcgcagggtg tcgagcagat cgccgttggc
    33061 gataataccc atgccacacg cctgaagtga ctggtagcaa gcgtagagcg gttcccacag
    33121 ttcttcgttt tgtgtcagca ggccttctgg ttttggcagt tcttcgcctt tcaggcgcgc
    33181 ttccagccat gcctgtgtcg ccatcaggcg agaacgcagg tttttcatca gatagcgata
    33241 cggttctgcg gcaccttctt cgccaaccag cgccagcagt tcaggggtcg cttcaaccat
    33301 cgacagttca gaaaccagaa cctgaatatc tttcaggaac agatcggtgg ctttccagcg
    33361 gctgagcagc aggacgtggc gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc
    33421 accgcccatc cacgaggtaa aacgtaccgg aacaaattcg acgggcagtt tgtagccgag
    33481 gttctcttcc agttgttcgt tcagttcgcg taggtaattc ggtacgcctt gccacaggct
    33541 gttttctact acggcaaagc cccatttggc ttcatctacc gggcttggac gcagcttacg
    33601 gatttcatcg gtatgccatg actgggcgat caactggcgc aggcgacgca tcagctggtt
    33661 gtgttcgtag tcagcgatat ctttgttatc gagctgtttt aagcaggcgt tcacttccac
    33721 cattttgtgg atcagtgtac gacgggtaat ttcggttggg tgagccgtga ggaccagttc
    33781 cagcgacagc gattccactg cttttttgat ggtgtcttcg ctcagttccg gctggttttt
    33841 cagtttacgc agggtgcggg cgatcacttc cgggttgctg gcagcttcgc ctttcggcga
    33901 aatgctgtgg tattgctcgg cggtgttggc caggttcagg aactgactaa acgcacgcgc
    33961 aacgggcagc agctcgtcgt tcgacaaatt ttgtaaggtg gtgagtaact cctggcggtt
    34021 agcatcattg ccagcgcgtg aagatttgga caacttacgg atagtttcta cgcgttcaag
    34081 aatgtgttct cccaacgcat ccttgatggt ttctcccagc actttgccga gcatactgac
    34141 attactacgc aatgcggaat attgttcgtt catattaccc cagacacccc atcttatcgt
    34201 ttgatagccc tgtatccttc acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg
    34261 tcgtctttta taaagccacg taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca
    34321 aacgaataaa tagcaggaat ttacgccatt aaattcacga cgctttaaat aagcgtaact
    34381 tatgggaatg ttaaaaaatc gcttcaagta acaccaaagg tgtaggtcgg ataagatgcg
    34441 caag
//
LOCUS       DAZUGK010000025        34362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZUGK010000025 DAZUGK010000000
VERSION     DAZUGK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.025"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(191..331)
                     /gene="yjjY"
                     /locus_tag="ACHY5W_001093"
     CDS             complement(191..331)
                     /gene="yjjY"
                     /locus_tag="ACHY5W_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFS4446030.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            427..1143
                     /gene="arcA"
                     /locus_tag="ACHY5W_001094"
     CDS             427..1143
                     /gene="arcA"
                     /locus_tag="ACHY5W_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFS4446031.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1203..2555)
                     /gene="creD"
                     /locus_tag="ACHY5W_001095"
     CDS             complement(1203..2555)
                     /gene="creD"
                     /locus_tag="ACHY5W_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFS4446032.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRNFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDQLRIWK
                     "
     gene            complement(2613..4037)
                     /gene="creC"
                     /locus_tag="ACHY5W_001096"
     CDS             complement(2613..4037)
                     /gene="creC"
                     /locus_tag="ACHY5W_001096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFS4446033.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQELVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4037..4726)
                     /gene="creB"
                     /locus_tag="ACHY5W_001097"
     CDS             complement(4037..4726)
                     /gene="creB"
                     /locus_tag="ACHY5W_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFS4446034.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4739..5212)
                     /gene="creA"
                     /locus_tag="ACHY5W_001098"
     CDS             complement(4739..5212)
                     /gene="creA"
                     /locus_tag="ACHY5W_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFS4446035.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5423..6292
                     /gene="robA"
                     /locus_tag="ACHY5W_001099"
     CDS             5423..6292
                     /gene="robA"
                     /locus_tag="ACHY5W_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFS4446036.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFTQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6289..6936)
                     /gene="gpmB"
                     /locus_tag="ACHY5W_001100"
     CDS             complement(6289..6936)
                     /gene="gpmB"
                     /locus_tag="ACHY5W_001100"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFS4446037.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6988..7509
                     /gene="yjjX"
                     /locus_tag="ACHY5W_001101"
     CDS             6988..7509
                     /gene="yjjX"
                     /locus_tag="ACHY5W_001101"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFS4446038.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7594..7920)
                     /gene="trpR"
                     /locus_tag="ACHY5W_001102"
     CDS             complement(7594..7920)
                     /gene="trpR"
                     /locus_tag="ACHY5W_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFS4446039.1"
                     /translation="MAQQSPYSAAMVEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8010..9947)
                     /gene="sltY"
                     /locus_tag="ACHY5W_001103"
     CDS             complement(8010..9947)
                     /gene="sltY"
                     /locus_tag="ACHY5W_001103"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFS4446040.1"
                     /translation="MEKAKQVTWRLLATGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEFRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10158..11825
                     /gene="ettA"
                     /locus_tag="ACHY5W_001104"
     CDS             10158..11825
                     /gene="ettA"
                     /locus_tag="ACHY5W_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFS4446041.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11979..12866)
                     /locus_tag="ACHY5W_001105"
     CDS             complement(11979..12866)
                     /locus_tag="ACHY5W_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001484212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4446042.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPADSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     FNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            13000..14010
                     /locus_tag="ACHY5W_001106"
     CDS             13000..14010
                     /locus_tag="ACHY5W_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4446043.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     RLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMCRQNLVAENAPLP
                     TR"
     gene            14030..14827
                     /locus_tag="ACHY5W_001107"
     CDS             14030..14827
                     /locus_tag="ACHY5W_001107"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4446044.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQSGV
                     DDAMAAIAAQTPTASLRGVVADLSTEDGVAALLAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            14853..15269
                     /locus_tag="ACHY5W_001108"
     CDS             14853..15269
                     /locus_tag="ACHY5W_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HFS4446045.1"
                     /translation="MAKTTVSIEIPASADAVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            15427..15639
                     /locus_tag="ACHY5W_001109"
     CDS             15427..15639
                     /locus_tag="ACHY5W_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFS4446046.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            15721..15834
                     /locus_tag="ACHY5W_001110"
                     /pseudo
     CDS             15721..15834
                     /locus_tag="ACHY5W_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16106..17338)
                     /gene="nadR"
                     /locus_tag="ACHY5W_001111"
     CDS             complement(16106..17338)
                     /gene="nadR"
                     /locus_tag="ACHY5W_001111"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFS4446047.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(17359..18741)
                     /gene="radA"
                     /locus_tag="ACHY5W_001112"
     CDS             complement(17359..18741)
                     /gene="radA"
                     /locus_tag="ACHY5W_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4446048.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(18790..19758)
                     /gene="serB"
                     /locus_tag="ACHY5W_001113"
     CDS             complement(18790..19758)
                     /gene="serB"
                     /locus_tag="ACHY5W_001113"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFS4446049.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            19864..20508
                     /gene="ytjB"
                     /locus_tag="ACHY5W_001114"
     CDS             19864..20508
                     /gene="ytjB"
                     /locus_tag="ACHY5W_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFS4446050.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            20536..21552
                     /gene="lplA"
                     /locus_tag="ACHY5W_001115"
     CDS             20536..21552
                     /gene="lplA"
                     /locus_tag="ACHY5W_001115"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFS4446051.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     KIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            21584..21847
                     /locus_tag="ACHY5W_001116"
     CDS             21584..21847
                     /locus_tag="ACHY5W_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4446052.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(22008..22727)
                     /gene="deoD"
                     /locus_tag="ACHY5W_001117"
     CDS             complement(22008..22727)
                     /gene="deoD"
                     /locus_tag="ACHY5W_001117"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4446053.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(22784..24007)
                     /gene="deoB"
                     /locus_tag="ACHY5W_001118"
     CDS             complement(22784..24007)
                     /gene="deoB"
                     /locus_tag="ACHY5W_001118"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4446054.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(24059..25381)
                     /gene="deoA"
                     /locus_tag="ACHY5W_001119"
     CDS             complement(24059..25381)
                     /gene="deoA"
                     /locus_tag="ACHY5W_001119"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFS4446055.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(25508..26287)
                     /gene="deoC"
                     /locus_tag="ACHY5W_001120"
     CDS             complement(25508..26287)
                     /gene="deoC"
                     /locus_tag="ACHY5W_001120"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4446056.1"
                     /translation="MIDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            26545..28095
                     /gene="yjjI"
                     /locus_tag="ACHY5W_001121"
     CDS             26545..28095
                     /gene="yjjI"
                     /locus_tag="ACHY5W_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFS4446057.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDVLLQPYVRILTQDEIDLRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHVAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            28067..28930
                     /gene="yjjW"
                     /locus_tag="ACHY5W_001122"
     CDS             28067..28930
                     /gene="yjjW"
                     /locus_tag="ACHY5W_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFS4446058.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(29346..30128)
                     /gene="yjjV"
                     /locus_tag="ACHY5W_001123"
     CDS             complement(29346..30128)
                     /gene="yjjV"
                     /locus_tag="ACHY5W_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4446059.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVLALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(30125..31198)
                     /gene="yjjU"
                     /locus_tag="ACHY5W_001124"
     CDS             complement(30125..31198)
                     /gene="yjjU"
                     /locus_tag="ACHY5W_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFS4446060.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(31320..31481)
                     /gene="ytjA"
                     /locus_tag="ACHY5W_001125"
     CDS             complement(31320..31481)
                     /gene="ytjA"
                     /locus_tag="ACHY5W_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFS4446061.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(31608..32213)
                     /gene="osmY"
                     /locus_tag="ACHY5W_001126"
     CDS             complement(31608..32213)
                     /gene="osmY"
                     /locus_tag="ACHY5W_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFS4446062.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(32606..34192)
                     /gene="prfC"
                     /locus_tag="ACHY5W_001127"
     CDS             complement(32606..34192)
                     /gene="prfC"
                     /locus_tag="ACHY5W_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4446063.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
BASE COUNT         8153 a         9398 c         8803 g         8008 t
ORIGIN      
        1 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
       61 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
      121 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
      181 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
      241 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
      301 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
      361 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
      421 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
      481 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
      541 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
      601 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
      661 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
      721 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
      781 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
      841 ttcaatggtt gggaactgga catcaacagc cgttcattga tcggccctga tggcgagcag
      901 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
      961 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accgcacgac
     1021 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
     1081 ccggaaatca tcgccaccat tcacggtgaa ggttatcgct tctgcggtga tctggaagat
     1141 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
     1201 ccttatttcc agatacgtaa ttgatcgtcc gttgtaacgt ctttactggc tttcattttc
     1261 ggcagtgaaa acgcatacca gtcgatatta cgggtcacaa acatcatgcc ggccagcgcc
     1321 accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag cagtccccac
     1381 atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg ccaacctttc
     1441 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
     1501 gccacggtaa aaccggtatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
     1561 gaaagcccca ccagcaaata ttgcattggg tgtaagcgtt gcgcggtgag cgtttcaaaa
     1621 acaaagaacg ccataaaagt cagcgcaatc agcagaatgg catacttagt cgcccggtca
     1681 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc
     1741 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
     1801 tgccattgcg cctgaaaacc tgactcacta acttcccgtt tggctggtag aaaatcacct
     1861 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg ccccgcaggc
     1921 accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctt caggttctgc
     1981 ttgcgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
     2041 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
     2101 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
     2161 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
     2221 acctgaccgg tataaatccc tatcttacgt tcttccacgt tctgattgcc atcaaccatc
     2281 aatgactcag gtaaccaaaa atggataaaa tttcgtttcc gctccacggt tttatcctct
     2341 tcctgcaccg tataaagctc ggtcacagga atagcgatga gcggcccaac gagtttttgc
     2401 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
     2461 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
     2521 gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc aatggcagca
     2581 ggatacgaag actatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
     2641 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
     2701 gcgacctcac tgacaaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
     2761 aaagagtaaa aacgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
     2821 tccagcacct taagggcgac gagttcctga tccacttcgg cgcttagcgt tatgcgaccg
     2881 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg ctccagtaac
     2941 gccggttcag cagcaacgtt aacctcagta ggcataacat gcaaagtgat gtttttttct
     3001 gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac atcaacagca
     3061 gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttctaccaat
     3121 gcctgcatac gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
     3181 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
     3241 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
     3301 atactttcca gcgcctgcgc aagcttacgc aactcgctac tgccgagttc aggaagagga
     3361 acgggcttat tgtcagtgac ggaatcggca tagcgagtga gccgggcaat ggagcggttg
     3421 atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccagcaaaat ggcgctggcc
     3481 cataaaattc gccgctcgct gcgcttaatg accggagcca tcgccgcgtt cggtttgcct
     3541 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
     3601 gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg gccacgcaac
     3661 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
     3721 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
     3781 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
     3841 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
     3901 gcggtgtcga tcagcgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
     3961 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcaacaac
     4021 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
     4081 tcggtgaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
     4141 cgacggtgcg atcgtaggtg tcctgcgcat cttcccatac gctatccatt agttgctggc
     4201 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
     4261 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
     4321 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
     4381 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
     4441 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
     4501 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
     4561 caacatcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc accggcaagc
     4621 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
     4681 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
     4741 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
     4801 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
     4861 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
     4921 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
     4981 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
     5041 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
     5101 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
     5161 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
     5221 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
     5281 caaaatctca atacttttat ttccgtcaag acctaaaaca tactctacta agaaaaaaac
     5341 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
     5401 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
     5461 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
     5521 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
     5581 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
     5641 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
     5701 gaagcagttt acccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
     5761 tattcgcccg ccgctgcgtc tgggtgaatt cactatgcca gagcataaat ttgtcaccct
     5821 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
     5881 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
     5941 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
     6001 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
     6061 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
     6121 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
     6181 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
     6241 agatcgccca attaatctgc gctgtgaact gctgattccg atccgtcgtt aacgctgcag
     6301 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
     6361 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
     6421 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccaggc atcccagtgc
     6481 aataccatga ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
     6541 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
     6601 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
     6661 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
     6721 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
     6781 gatgatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
     6841 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
     6901 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
     6961 acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac caccaatccc
     7021 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
     7081 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
     7141 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
     7201 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
     7261 aacgccagcc agcgcggcga ggcgcgttcg gctactttgc cgttgcctgc ggtgattctg
     7321 gaaaaagtgc gtgaaggcga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
     7381 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
     7441 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcataatgc cgtgtatcag
     7501 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
     7561 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
     7621 cagccactgg cgcagctcaa cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
     7681 cgcgatgccc gcgccgagtt cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag
     7741 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
     7801 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
     7861 taaccactcc tggtgacgct gttctaccat cgctgctgaa tagggtgatt gttgggccat
     7921 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccgattgta ctcgctaaag
     7981 agtacgatag catatcataa acgtgtggat cagtaacgac gtccccattc cgtggcgctc
     8041 atcaacgtcg gtttatcccc catgaaatag cggtagtaag cgtcataagc cagcacgttc
     8101 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
     8161 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
     8221 gaggagaaaa tacggttatt accaaattgc tgataaacat attgcaggta actggtgcca
     8281 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
     8341 aacatcttca ccgtatgggt cgccgtgccc ggcataatct gcatcaggcc gctggcccct
     8401 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
     8461 ctttgcggga tctccttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
     8521 aatcgctctt ccagatgatc ccacagcttc ccggcgatcg ttgcctgaac gctaagatcc
     8581 caccattggt tgttgaaagc ataccgcgcc agttgagcct gctctgtttt tgacttgctc
     8641 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
     8701 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
     8761 tcaatcttca gctcatactc ttcgccgatg cgttgtgcag caaccatcgg gtagaaacca
     8821 cgctgttgca tgagttgatg caaaatctct tttgcttcag cttcacgtcc gcgttccagc
     8881 aataaatccg cctgccagta acgccattca tctttctctt tcgcttccat aggcagacgc
     8941 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg cacacggcgt
     9001 tcaataagcg aagtagattg cgagcgcata atggcgtcat cgcgccattt cgcctgctcg
     9061 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
     9121 tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc attttcagca
     9181 tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa atcggtcgct
     9241 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
     9301 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
     9361 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
     9421 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
     9481 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
     9541 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
     9601 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
     9661 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcagg
     9721 tcatcggtga tctggcggaa ttccaggtag ggataaagcg gataatcctt cagtccaggc
     9781 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
     9841 cgactacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
     9901 acaccggtag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
     9961 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    10021 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    10081 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    10141 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    10201 ttccgccgaa acgtcatatt ttgaaaaata tctctctgag tttcttccct ggggcaaaaa
    10261 ttggtgtcct gggtctgaat ggcgcgggta agtccaccct gctgcgcatt atggcgggca
    10321 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttatctgc
    10381 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    10441 cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    10501 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    10561 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg cgtctgccgg
    10621 actgggacgc gaaaatcgct aacctctccg gtggtgaacg tcgtcgcgta gcgttgtgcc
    10681 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    10741 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga
    10801 ttacccacga ccgttacttc ctcgataacg ttgcgggctg gatcctcgaa cttgaccgcg
    10861 gtgaaggtat tccgtgggaa ggtaactact cctcctggct ggagcagaaa gatcagcgtc
    10921 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    10981 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    11041 aagagctgaa cagcaccgaa taccagaaac gtaacgaaac caacgaactg tttattccac
    11101 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg
    11161 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcgatc gtcgggatca
    11221 tcggtccgaa cggtgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    11281 cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    11341 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    11401 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    11461 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    11521 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    11581 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    11641 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    11701 aggatgaagg taaagttgag ttcttcgaag gtaactttac cgaatacgaa gagtacaaaa
    11761 aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag cgtattgcga
    11821 agtaatgcgt aaaatgccgg gtgcggcgcg ccagcatcgc atccggcatt agttaaatga
    11881 tgagtccccg aaaaggcaga attctcttta agtgtattag ctcagatgcg gactgacatc
    11941 tcaaatcaag cgggaactca tggttcccgc ttaacgtttt attcaccgcg caggatctgg
    12001 cggaaatggg aaaccagcgg acgttggttg gctttatgcc aggctgccca taattgcgtt
    12061 gaataattca tccacggcaa ttcacgtaat accacccctt ccggcgcatt atgacgcaga
    12121 ctatgttgaa ttaaggccag acctaatccc gcagcgacca gacctaatgc cgtgaggggt
    12181 tcacccgctt ccaggcgaat atcaggatta aacccggcgt taacacaggc ggcgacaaaa
    12241 tgatcgcgag aatgcgcatc gtcgttatga atcaccgcaa tccactgctc atcgccaagt
    12301 tgtgacgcga caaggttttc ctcctgcgcc agaggatgat tttgcggtaa ggccaacaac
    12361 attcgatcat ccagaacctg aatatattcc agatcctgct cgctgtcggc gggcggcgta
    12421 tcgaccagag aaaaatcgag actgcgctgg cgcaggcctt caatctgggt agccggtggt
    12481 tggctgtaaa gagcgatatg cacattcgga cgcgatacac gtaacgtacg taacgccgta
    12541 gaaagtacac cggaatgcat ggcgttttcg atatagccaa tacacaagcc cccatcttcc
    12601 ccccgtccca ggcgtttgcc taacgactcc aggcggttgg cgtgtgtcag caacgaacgt
    12661 gtttcggcaa ggaatgtctg tccatcagcg gtcaggcgaa tgcgctgctg attacgctca
    12721 aacagcgtca gccccagtcg ttcttccagt tgtgcaatct ggcggctcag cggcgattgc
    12781 gagatatgta atcgctcggc tgcgcgtccc acgtgctctt cttctgcgac caccacgaag
    12841 tacttcagtt ggcgtaaatc aatcatgtta gacctcaaag gacttaagtc agacgaatta
    12901 tgtcctatca agtcacaacc tcgcaaggta ttgtgatcca cgtcacaggg cgatagcaaa
    12961 aaaacgccct aacccaaacc gaataattga ggacatctta tgagcattaa aaatttactc
    13021 cccggcaaaa ttggcttagg cggcgcacca cttggcaaca tgttccgtgc cattccggaa
    13081 gaagaggccc acgccaccgt gagcgaagcc tggaacctgg gtgtgcgtta ctttgatacc
    13141 gcgccgctgt acggctctgg cctttctgaa atccgcatgg gtgaagcgct gtctcagtat
    13201 ccacgcgatg aatacgttct gagtagcaaa gtcggtcgca tcatgctgga cgaaatggaa
    13261 gatccggcaa aacgggattt cggtgaaaaa ggtggcctgt tcgaacacgg cctgaaaaac
    13321 aaaattctga atgactactc tgctgacgcc acgctgcgat cgattgaaga cagcctgaaa
    13381 cgcctgaaaa cagaccgtct ggacattgtc tggatccacg acccggcgca ggacttctac
    13441 ggtgacagct ggctggagca gttcaacatt gcccgtaccg gtgcatttcg tgcgctgacg
    13501 cgtttgcgtg acgaaggtgt gattaaagcc tggggactgg gtgtgaaccg cgtcgaacct
    13561 tgcgaactga cgctggcgct ggacgaaccg aagccggatg cgttcctgct ggcggggcgt
    13621 tattccctgc tggatcacga acgtgcgctg caacgcctga tgccagaagc actggaacag
    13681 aacgtggata tcgttgtggg cggtccatac agctctggcg tactggcagg cggcgagcac
    13741 tttgaatatc agaaagcctc tgcggccatc cagcagaaag tggcgcaaat taacgccatt
    13801 gccacacgtt tcaatgtgaa cgttaaagcg gctgcgctgc agttcgcgct ggcaaacccg
    13861 gccgttgcag cggttattcc tggctccagc cgtcctggcc gtatggcaga agatctggct
    13921 gcactgaatg cgcctgttcc ggctgaattc tgggcagaaa tgtgccgtca aaatctggtt
    13981 gcggaaaacg ctccgctgcc gacccgttaa tcaacctggt gagaaaacga tgaaactgga
    14041 tctgacaggt aaagtcgcgg tggtcagcgg ctcaacctcc ggcattgggc agggtatcgc
    14101 cgccggactg gcaaaagcag gcgcaacggt ggttgtcgtc ggccgtaaac agagcggcgt
    14161 tgatgacgcc atggctgcga ttgcggcaca aactcccaca gcgtcactgc gcggcgtggt
    14221 ggcggatctc agcaccgaag acggcgtggc tgcgctgctc gctgcagtcc caacggctga
    14281 tgtgctggtc aataatctgg gcatttttaa cgataaagat ttctttagcg ttccggatga
    14341 tgagtggatg cacttctata acgtcaacgt cctctccggc gttcgtctgg cgcgtcacta
    14401 tgcgccaggc atgacggaac agggctgggg gcggattatc tttgtctcgt ccgaatccgg
    14461 cgtggcaatc cctggcgaca tgatcaacta cggcgtcacc aaagccgcga atctggcggt
    14521 ttctcatggt ctggcgaaac gactggcggg gaccggtgtg accgtcaacg ccattctgcc
    14581 agggccaacc tttaccgatg gcctgaaaga gatgctggcg gatgccgctg cacaatcggg
    14641 acgcagtgcg cgtgaacagg ccgatgagtt tgttaaaacc tcgcgtccga gttccatcat
    14701 ccagcgtgcg gcagatgtgg aagaagtggc gaatctggcg gtttatctcg cgtcaccgct
    14761 ttcctcggca accacagggg cagccctgcg cgtcgatggc ggcgtcgtcg acaccctgac
    14821 aatgtaaccc tataaattaa gagaaaatta tcatggctaa aacaactgta tcgatcgaaa
    14881 ttccggcttc tgccgacgcc gtatggcaat tgatgggcgg ctttgatgcg ctgccagact
    14941 ggctgccgtt catcccgaaa agcgtagtga cagaaggcgg tcgcgttcgt tcgctgacaa
    15001 cctctgacgg cggcactgtg gttgaacgtc tggaagcgtt tgataaccgt caacgcagtt
    15061 atacctattc cattattcag gctccgttcc cggtggtgga ttacctctcc accattaccg
    15121 ttcatgaaac cgccgacagc caggtttccc gtgtggaatg gttcggtgaa ttcaccccgg
    15181 tgaatgtgac gaacgaagag gccgaggcgc tgtttaccgg catctatcgg gatggtctca
    15241 aggcgctaaa ggacaacttt gttgcctgat gcgatcgaca ggcgtcggaa cgtccggcgt
    15301 ctgattatta acaaaaacag aatagtctat tgccatttgt atcatttatg ttatcaggca
    15361 gcaggcgtat tctctcccca tcgcgttaat aaacgccaaa caattaagac actctggaat
    15421 ataaacatga aaacgatcat cgcaattttt acggcttctc ttttctctgt cgcggcgttt
    15481 aacgcatcgg cgcaaagcgt gacagcaacc ggtacaacac tggattccgc tgaagcggtt
    15541 attgcgcaga aagcgaaaga gatgaacgcc tctgaataca aaattaccag cgctcgcatg
    15601 ggcaataccg tcaccatgag tgccgaactg tataaataat cgcgcgagga acccattacg
    15661 taatttgcta aatatgcgct gaatgcgttg ccattaataa accgtattgt actgccaccg
    15721 atgacgcgtt cccgcgccgg tgcggttgac gttgcgattg acatgatggc ggagtattac
    15781 gcgcagcgcg ccagtgctgg acttatcatc tgcgaaggta cgcaaatcag cagaagcgct
    15841 gcgcataatt ttccacggca cgctgattta ctccggtaaa tacaccaaag cgcgcgccga
    15901 agcgaactgt cagatttgat tgcattctgg ctacgcaacc ggtcggttgg agtatgaagt
    15961 tgctcacgaa tagccttagc atccgcaatt tcgtagcggg tcacccgtac aggttctttc
    16021 taatctcgac agtctcttcc agagaggttt tcaactcatc aaaaaaactc attttatacc
    16081 tcgcctttga gcagtttcgt cacggttatc tctgctcccc catcatctcc cgcaccagct
    16141 ccacgcagcg caggaaacgg ctgtcgtaat cttcctcttc aacccgcaca aattcgatat
    16201 tgttctcttc gagcatctcc accagcaagt tctggaactc tttgcgatcc accgaactgc
    16261 cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg atcaccagat
    16321 caaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcgcgtccc tcgtactttt
    16381 tgcagaacgc ctgagtggtg acaaaatcgg tatcgataaa tgccacttta ttggcatatt
    16441 tcaccgcaaa atcaatgtat tgagcgtggc ccagtgcgat tttatcgtag tcagaatact
    16501 gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca tattcccacg
    16561 cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta ccgctcgact
    16621 cgccgccaag gatcgccacg gtacgcacaa agaacggctt cacttccgta ggaatatatt
    16681 cccagtagcg gaacgggttt tcgcggatct gtgcaccgct gatactcata aaggtacgtt
    16741 tcggatctac cagcaccgtt tcgatcccca gatgttccat atactgtggc gcatcggctt
    16801 cttccgaggt gtagatcagg tccggctgaa tccccttttc agccataaac tttttgatgc
    16861 cgttgctcca cacatcccag ccgtgcggat acggctccat gccctcttcg ttgaaagcat
    16921 gaatgcgaat atttttctga tatttaaaag tttgcaataa ccagcgcaga cgatccggca
    16981 cggtcggctg ctgcgacatg gcactgtctt cgaacaacgc gcggtcacgg gtatcgtcaa
    17041 aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata aggtagatat
    17101 gtccggtatg cagtgggtag aacttaccga atactacacc aatggttttc ttctgccggg
    17161 gaaactcaag ccctaaaaaa cggtgcaacg cctccagctt ttgcgcgctg gggcttttaa
    17221 ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca tcagctacct
    17281 gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat gacgacatat
    17341 ctccctccgt atttctcatt ataagtcgtc gaacacgcta agcgcgtcgg agagtttttt
    17401 aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg ccggaacaat
    17461 cgcccggcga aaaccgtgtt tcgccgcttc agagattcgt tcctgaccgc tgggcaccgg
    17521 gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgcg gtagcggtct
    17581 gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcggcgctgg tttcggttac
    17641 cttaacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca gaccaccgtg
    17701 acggtgcaaa acagccagca ggattgccag acggttttgt tccagcccca ctgccacgcg
    17761 gcgcggattc gccatcatcg agtgatcgac cagcgcctga atctccacca gcaacggacg
    17821 cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc cgcgacttaa
    17881 gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg cgaagacgcc
    17941 cagctcattc accgcgccga agcggttttt atggctgcgc aaggtgcgaa aacgggagtc
    18001 ggcatcgcca tccaaaagca ccgaacagtc gatacagtgt tcaagcactt tcgggccagc
    18061 cagcgaacca tctttggtta cgtgccccac catgacaatc gccacaccgc gcgttttggc
    18121 gaagcgtgtc aaataagccg ccgtttcacg cacctgcgcc acgctaccag gcgatgactg
    18181 tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg gttgctcttc
    18241 ttcggcaatc aggcagatct gttcgatgct ggtttccgac aacatattga gattgtcagt
    18301 cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt cgccggtgac
    18361 atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagtagca gcgtggattt
    18421 ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc cgccgcctag
    18481 tacgcggtcg aattctttaa atccggtgga aaaacgcggc agctcttcaa ggctgatatc
    18541 ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga gacgctcgtt
    18601 acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc aggcatgaca
    18661 ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac aaacaaaggc
    18721 gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg caagatggcg
    18781 ggcgagcaat tacttctgat tcaggctgcc tgagaggatg cagaataccc ccatcaggtc
    18841 agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat ggtaggcaat
    18901 ccccagccct gccgctttga tcatcggcag gtcattggct ccatcgccaa tcgccacggt
    18961 ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg ctttgtactg
    19021 cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga tctccagttc
    19081 attggctacc acggcggtca ggcgcagctt gtcgcgcagg tattcagcaa agaaagtaaa
    19141 gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca ccagttgcgt
    19201 taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg cgtcagcgcc
    19261 tttcagcgtc gccacacggc tgcgcaggct ggcggtaaaa tcgagttcgc cgcgcatcgc
    19321 ccgttcggtt acttccgcca ccatctcgcc cgttccggcc agtttggcaa tttcatcaat
    19381 acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg gcgtgcgcag
    19441 gtgcgggatt ttccccagcg gggcgacatc cagttgcgct tcatgggcca ggcgcgtagc
    19501 ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc accaggcggc
    19561 aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct gtttatccag
    19621 cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg gcatcacttc
    19681 atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag gcaggtcgca
    19741 ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga aaataacgca
    19801 tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt caaagggtgg
    19861 aagatggctc gcacaaaact gaaattccgg ctgcatcggg cagtgattgt cctgttctgt
    19921 cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa ccaccagcga
    19981 cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt gacgctgaac
    20041 gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc gatcctcgat
    20101 cagttaacgg atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca aggcgatctt
    20161 atcgcacgtt ctggcgaaag cgtcgaagtg cgcgaccggc tggcgctcga cggtaaaaaa
    20221 gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa cggaccgctc
    20281 ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca acaggtggat
    20341 aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg cgtagtgcta
    20401 acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt cctgttaacc
    20461 gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc cattactaca
    20521 agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta cgacccgtgg
    20581 tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca gcgcgttctg
    20641 tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc gtggaaagag
    20701 tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag tagcggtggc
    20761 ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg caagccggag
    20821 tacgataaaa ccatctccac gtcgattgtg ctcaatgctc tgaacgcgct cggcgtcagc
    20881 gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg ccgaaggcga ccgcaaagtc
    20941 tcaggatcgg cctatcgcga aaccaaagat cgtggcttcc accacggcac cttgctgctc
    21001 aatgccgacc ttagccgcct ggcaaactat ctcaatccgg ataaaaagaa actggcggcg
    21061 aaaggcatta cgtcggtacg ttcccgcgtg accaacctca ccgagctgtt gccggggatc
    21121 acccatgagc aggtttgcga ggccataacc aaggcctttt tcgcccatta tggcgagcgt
    21181 gtagaagcga aaatcatctc cccagacaaa acgccagact tgccaaactt cgccgaaacc
    21241 tttgcccgtc agagtagctg ggaatggaac ttcggtcagg ctccggcatt ctcgcatctg
    21301 ctggatgaac gctttacctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat
    21361 atcacccgcg cccaggtgtt taccgacagc ctcaaccccg cgccgctgga agccctagcc
    21421 ggacgactcc aaggctgcct gtaccgcgca gatatgctgc aacaggagtg cgaagcgctg
    21481 ttggttgact tcccggaaca ggaaaaagag ctacgggagt tatcggcatg gatggcgggg
    21541 gcggtaaggt aactcgctga ttaaaatatc cagggaagga aatatgatcg atctggaaaa
    21601 ccaggaacgg gagattatca atttaatgtt cagccagcgt atttcctggc tagccgctgt
    21661 gcgtatcagg cataaattat cgcttgctga agtaagtaaa atgttaggta tctcgattaa
    21721 ttctctaaaa cagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggc
    21781 agaaatttat ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaaca
    21841 taaatgaata gattcgtgaa tagatacttg aatgtatttt attttctatc catatgaaaa
    21901 ataatagtaa atctataaat cagttgctat tgatgaatag atactaaaaa gccggagcag
    21961 tctcccgctc cggcttaaca caacaatcgc ctttcagcga aacacaatta ctctttatcg
    22021 cccagcagaa cggattccag tgcgattttg atcatgtcgt tgaaggtggt ctgacgctca
    22081 gcggcagtgg tctgctcgtg agtgcggatg tggtcagata cggtgcagat ggtcagggct
    22141 ttcgcgccaa attctgcagc gacgccgtag ataccagccg cttccatttc cacgccgaga
    22201 atgccgtatt tttccatcac gtcgaacatt tcgccgtccg gagagtagaa caggtcagcg
    22261 gagaacaggt tacccacgcg agcatcaatg cccagtgctt tagctgcatc tactgcgtta
    22321 cgcaccatgt cgaagtcagc gatagcggca aagtcatggt ctttaaaacg gatgcggtta
    22381 actttggaat cggtgcaggc acccataccg ataacgacgt cgcgcagttt tacgtgcggc
    22441 agaactgcgc cacaggaacc cacgcggata attttcttca cgccgaaatc ggtgatcagt
    22501 tctttggtgt agatggagca ggacgggata cccataccgt gacccattac ggaaattttg
    22561 cggcctttgt aagtaccggt gaagcccagc ataccgcgaa cgttgttcac ttcacgggca
    22621 tcttcaagga aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg catcaaaact
    22681 acgtcagcga aatcgcccat ttctgcatta atgtgtgggg tagccattgt tttatccttt
    22741 ttgtgacata acaaaggcgg agtcaacgct ccgcccaaat ccatcagaac atggctttgc
    22801 catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg
    22861 tttcacgatg acccagtgag cccggtttta ctttcgggcc gtaaaccagt accggaatgt
    22921 gttcacgcgt gtggtcagta ccggtccagg tcggatcaca gccgtggtca gcggtgagga
    22981 tcaggatgtc gtcatcgcgc agtaaagaca tcagctccgg cagacggcgg tcgaacagtt
    23041 ccagacccgc cgcataacct gcgacgtcgc gacggtggcc ccaggaagag tcgaagtcaa
    23101 cgaagttggt aaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt
    23161 caaacagcgc gtccaggccg gtcgctttca cttttttggt gataccgcag ttggcgtaga
    23221 tgtctgcaat tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc agtttctgca
    23281 gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta cgctggaagt
    23341 taccggcttt gtcgccgata aacggacgag cgataacacg accgatattg tagccgccgt
    23401 tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaga ccgaaagttt
    23461 cttcatggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc ggcttgccgg
    23521 ttttcatgtg ctcttcgccc agttgatcca gaatgaccgt accggaagag tggcagttac
    23581 cgaggtaacc cggcagatta gcgcgttcga ccagtttatc cagcagctct tgcgggaagc
    23641 tgttttcgtg atcggagaaa tatccccact caaacagaac cgggacaccg gcaatttccc
    23701 agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga
    23761 taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt
    23821 tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga ccgttatcag
    23881 cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa
    23941 agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac
    24001 gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt
    24061 attcactgat acgacgatag acagttggtg tgctttccgg tgctttatcg gcaagtttaa
    24121 ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgtggataa
    24181 ccgccagcgg acgctgaccg tctacctggt cgcccagacg tgccatatca gtaaagccga
    24241 cgctgtaatc gatggtgtca gatgcctgac ggcgtccgcc gcccattgca accactgcca
    24301 tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt
    24361 tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gtcgggcctt
    24421 tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc agcaccgcct
    24481 gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc agcatctcca
    24541 cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattcaccc gtcaggaact
    24601 gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt
    24661 cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg
    24721 caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact ttcacgtcca
    24781 tcaccagcgc gtccagacct tccgcaagtt tcttcgccag aatagaggcg gtgatcagcg
    24841 ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tccgccggag
    24901 ccagtgagct ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac
    24961 ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc
    25021 cagtatgacc gaggccgcga ccagagatca tcggaatata gccgccgcag gctgcgacca
    25081 tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtcgagtgt ttatcaacaa
    25141 tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca
    25201 tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg
    25261 cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag aaacgaattt
    25321 cttcatcgct cagcgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca
    25381 aggtaacctc ccgtggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat
    25441 ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc
    25501 atcttactta gtagctgctg gcgctcttac cgtcgccgtg acccagcgct ttcagcaggc
    25561 ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atcagcccag tcagcaccga
    25621 acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg
    25681 ccggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc atcatgatgc
    25741 gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg
    25801 cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc
    25861 cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt
    25921 tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac gggaacacca
    25981 cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga
    26041 tgtcgtcgtt accgtgtggg aagttggtta ccgtagcaat acggatttcc ggggtgccct
    26101 gctctttcag tgttttgcga gcaatcggga taaagcgagg atagatacag atagcggcgg
    26161 tattgccgac cggggttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt
    26221 cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat
    26281 caatcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat
    26341 tctaacatct actccgcaac acacttcgat acacatcaca attaaggaaa tctacttaca
    26401 agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag
    26461 tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca
    26521 ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt
    26581 tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca
    26641 ctggaagcag aaaacaatct gccttaccca cagcttcctg ccgaagcccg ccgcgcgctg
    26701 gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc
    26761 ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg
    26821 aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc
    26881 acatcgatgc cggtctacct ggggcaactg gatgtgttgt tgcaaccgta tgttagaatt
    26941 ctaacacaag acgagatcga tcttcgaata aaacgtttct ggcgttacct cgacagaacc
    27001 ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgtgca
    27061 atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt tatctacgat
    27121 cctgaaatca cccctgatga cctgctgctg gaagtggcga aaaacatctg tgaatgtagc
    27181 aaaccgcacg tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    27241 atagtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    27301 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    27361 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    27421 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    27481 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgaaaaa
    27541 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtagg ttatcgcatc
    27601 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    27661 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    27721 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    27781 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaat
    27841 ccgcaggcgc tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    27901 gctaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    27961 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    28021 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    28081 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    28141 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    28201 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    28261 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagag
    28321 gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggatgagg tgcttagcca
    28381 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    28441 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    28501 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    28561 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    28621 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    28681 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    28741 cgaagaactg gcggcgttta tcaagggact tggcgatgtt ccggttcgcc tgaacgcgtt
    28801 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    28861 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    28921 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    28981 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    29041 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgacaag cgtcgcatca
    29101 ggcattatgc gccgactgcc ggatgcggcg tgaacgcctt atccggccta cggttcggca
    29161 cagacttgta ggcatgataa gacgcgacaa gcgtcgcatc aggcattgtg cgccaactgc
    29221 cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt aggcatgata
    29281 agacgcgtta agcgtcgcat caggcattat gcgccgactg ccggatgctg cctacgaagg
    29341 gcaaactacc cacgaacgtt aaacaccgca tacgtgttat taagcaacac ttcggcaatc
    29401 tcatccgcgg gttccgggcg taactcgcaa agcacggcaa acacacgggc agcatgctcc
    29461 gggcggttag gctgcccctg aaaaccgttg agcggcatat ccggcgcgtc ggtttccagc
    29521 aataacgatg ccagcggtaa ttttgcgatg acatcgcggg ttttactggc gcgtggatag
    29581 gtgatagtac cgcctacgcc gattttgtag cccagctgta caaaccgctc ggcctgttgc
    29641 aggctgccgg aaaaaccgtg aaccacgcca gtgcgcgata aatcgtggcg tttaagatgc
    29701 atcgccagtt tgtcgtgcgt gcgccgtgaa tgcaggatca ccggcagatc gtagcgtttc
    29761 gccagtttca gttgttcgtc gagtaaccac tgctgcctct caaattgcgg atcgtcgccg
    29821 aagagatcca gaccgatctc ccccaccgca accaccttcg ccggacgcct ttccagcgcc
    29881 tgctgtagct gttcaagaga cacatcgcta tgtttttcca acataccagg atgcaagccc
    29941 aatgcggcat acagcggttg ataattttcc gctaatgcca acacacgggc aaaattttcc
    30001 gcctctgtcg ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg ttgcaggctg
    30061 gcctcttcat cgccactaaa cggcgggaaa tcaaaatggc agtgggtatc gataaaacgg
    30121 caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga gcgtcgctca
    30181 cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg aatgacaatc gattccggcg
    30241 tcaccactgg caccagatgg cgggtaagcg gcgctttttc agttaatagc ttgccaaccg
    30301 tggcgaggaa ataacgaccg cataaacgcc caagtttata gtcttcacgc agcgccggaa
    30361 tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg aatatccgca
    30421 gcttgcccgg tggtttctca ataaattgct gaatgtcacg atagctggtt tcatgatgct
    30481 gcaccagatt gaccagcggc tgcaggctac tgtcacccag ccagcgttcc atgcgtttga
    30541 accactgcgg cgtgtagtac atttgtgacg gcacagtgcg gatgacgacc aacgttttag
    30601 cgccctgcct tgccgcctct ttaaccggaa tcgcatcact gatcccgcca tccaggtagt
    30661 taatgccttc cagcgacact ccgctacgat aaaagccagg tatcgcactg gaggcgcgaa
    30721 tcacatccag ccagttttgt ttggttggta aaaagtaatt cggcgcgtag tcatcctgac
    30781 gacaggcgca catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg gtatccattt
    30841 gcaacggcat ctggcttgcg gtggcctcca ccagccagtc gagatcgata agatttcctc
    30901 cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc atgatgactt
    30961 tgcgcgcgta accgggctga ttgcagataa acgccgagag gttctgcgcc ccggcagatg
    31021 tgccgagata aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc agcacgccag
    31081 ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag agctattcgt ccaggctgga
    31141 acggccttag cgacaacggc gcaatattac caagcgttac aggtattcgc tgccccacct
    31201 ctgccttcct gtttttgttg tgaaagaatg acaaagtaac gcaatttatt attcgctaaa
    31261 accgcaaaag ccagtccgct ggactggctt tggataaaat tttcttaatg gtctgaaatc
    31321 tagggtcgtt ttcggcccat gaacaaactc accaggaaca gaataatccc gacgacaaag
    31381 acaattttag ctgcgcctgc agcggtaccg gccagaccac caaacccaag tgcggcggcg
    31441 attaacgcga taaccagaaa tatgatgccc caacgaaaca taagcgtctc ctttaccata
    31501 gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt ttagtgtggt
    31561 gcacatcgcg cctcccgaca aagttcggga ggacgaatta cgacgaatta cttagttttc
    31621 agatcatttt taacgctttt cacaccatct accgctttgg cgatactttc agcacggtca
    31681 ctttgtgcct gagaatcgac ggtaccggag agctgaacca cgccgtcggt ggtttcaact
    31741 ttcacatgac gggaagggac gatatcgtcc gccagcagtt tggctttgat ttcactggtg
    31801 gtggcggtgt cacccgcgta gcccttcacc gagccttctt tagcgtcgcg aacgtgcagt
    31861 ttgtcgctga cagaggtcac cccttcaacg cctttcgcca ctttcactgc ctcttcggcc
    31921 tgggcctggc tttcaacgaa accgctcagg gtcacgactt tttgatcggt ttttacagag
    31981 atatcggtgc tcttgatgtt gtcatgatcc accagggccg ccttcacttt cgcggtgatg
    32041 gcgctgtcat ccatgaaatt accgacttta ttcatagagc tatcgacttt ttgccctgcg
    32101 ctttcattgg tagtctgcgc gttgttttcc gcgtaggcag agccggtcgc gacggcagag
    32161 gtcaacatta cagccagcag agttttcgaa atcttcagtc ttgtcatagt catcgattta
    32221 ttcctgtatg tttgctcgta atttgagcct ggcaacacga ggttgcattg ctgaataggg
    32281 agagacttca ccctctacag aagtcaatgg tcgccatcac aaaagcgatg agtgatgaat
    32341 aacgaccatt acagcctctg aatcagttat taatatcggt agaatgacaa tcgacggctt
    32401 tagatactga tatctacgca ttgaacggta tttaacgccg tcagaaatgt catcactttg
    32461 ttaaatatag ttcacaattt tgaaaccgct cgggatatca gcgaaaacat aagcaaaagt
    32521 gaatgtttta agaacattcc gtaagcggct aataaggaag ggaaattgac agggcgcagc
    32581 ggctaccgcg ccctggaggc aagaattaat gctcgcgggt ctggtggaac tgaacgtccg
    32641 gataacgttc ctgtgccagg cgcaggttga ccatgctggt agcgatgtaa gcgaggttat
    32701 cgccgccatc aagcgccagt tggctttcgt tcttacgctt gaactcttcg aatttcttcg
    32761 cgtctgcaca ttctacccag cgggcagtgg caacgttgac tgactcatac actgcttcaa
    32821 cgttgtattc gctcttcagg cgcgctacca ccacatcaaa ctgcagcaca ccaactgcac
    32881 caacgatcag atcgttgttg gagattggac ggaacacctg caccgcgccc tcttcggaaa
    32941 gctgtaccag ccctttgagc agctgttttt gcttcagcgg atctttcagg cggatacgac
    33001 ggaacagttc tggtgcgaag ttcggaatac cggtgaactt catcatctca ccctgggtaa
    33061 aggtgtcgcc gatctgaatg gtgccgtggt tgtgcaggcc gaggatatcg cccggatacg
    33121 cttcttcaac gtgcgaacgg tcacccgcca taaaggtcag cgcgtcggag atcaccacat
    33181 ctttcgcagt gcgcacctgg cgcagtttca tgcctttttc atatttaccg gacaccacac
    33241 gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt ggcctgaatt ttaaatacga
    33301 agccggtaaa tttatcttcg ctcgcttcta cggtacgggt atcggtctga cgcggcatcg
    33361 gcgcaggcgc ccactccacc aggccatcca gcatatgatc aacgccgaag ttacccagcg
    33421 cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa cagctctttg tcgaactcgt
    33481 tagacgcgcc tttaaccagt tccagttcgt cacgcagctg ctgtgccaga tcttcaccaa
    33541 ccgcagcatc gagatccggg ttattcagcc ctttaacaat gcggacttcc tgaatggtgt
    33601 gccctttacc gctctgatag agataggttt catctttata aaggtggtaa acgcctttaa
    33661 acagcttgcc gcagccaatt ggccaggtga tcggtgcgca gccaattttc agctcgttct
    33721 caacttcatc gagcaattcc atcgggtcgc ggatatcacg gtcaagtttg ttcataaagg
    33781 tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag cttacgggta cgatcttcaa
    33841 caccttttgc ggcgtcgata accatcaggc agcagtccac cgccgtcagg gtacggtagg
    33901 tatcttccga gaagtcttcg tgccccgggg tgtcgagcag gttaaccagg caatcgtgat
    33961 acggaaactg catcacagac gtagtaatgg agatcccacg ctgcttttcc atctccatcc
    34021 agtccgactt agcgtgctgg ttggaaccac ggccttttac tgtaccggca gtctgaatgg
    34081 cctgtccgaa cagcagcacc ttctcggtga tggtagtctt accggcgtcc gggtgagaaa
    34141 taattgcaaa ggttcttctt ttcgctacct ctgcggccaa aaggcttgtc ataattgttg
    34201 tctctttgat tatgtacaat catatgtaca aagaaaaatt catgccgctg attgtacatt
    34261 ttggaggtat cagagcatag cagaacatga tcagtaggtg tatttgtagc caaagaacat
    34321 ttgtacatgc agcaatcttt gttctattga cactgtttat tc
//
LOCUS       DAZUGK010000026        34279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZUGK010000026 DAZUGK010000000
VERSION     DAZUGK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.026"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(193..846)
                     /gene="ribB"
                     /locus_tag="ACHY5W_001128"
     CDS             complement(193..846)
                     /gene="ribB"
                     /locus_tag="ACHY5W_001128"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFS4446064.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(951..1099)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1221..1520
                     /gene="ubiK"
                     /locus_tag="ACHY5W_001129"
     CDS             1221..1520
                     /gene="ubiK"
                     /locus_tag="ACHY5W_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFS4446065.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1555..1755)
                     /gene="glgS"
                     /locus_tag="ACHY5W_001130"
     CDS             complement(1555..1755)
                     /gene="glgS"
                     /locus_tag="ACHY5W_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFS4446066.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            2024..2652
                     /gene="yqiJ"
                     /locus_tag="ACHY5W_001131"
                     /pseudo
     CDS             2024..2652
                     /gene="yqiJ"
                     /locus_tag="ACHY5W_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
     gene            2679..4340
                     /gene="yqiK"
                     /locus_tag="ACHY5W_001132"
     CDS             2679..4340
                     /gene="yqiK"
                     /locus_tag="ACHY5W_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4446067.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            4579..4638
                     /locus_tag="ACHY5W_001133"
     CDS             4579..4638
                     /locus_tag="ACHY5W_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4446068.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4955..5014
                     /gene="ibsE"
                     /locus_tag="ACHY5W_001134"
     CDS             4955..5014
                     /gene="ibsE"
                     /locus_tag="ACHY5W_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFS4446069.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(5134..6567)
                     /gene="hldE"
                     /locus_tag="ACHY5W_001135"
     CDS             complement(5134..6567)
                     /gene="hldE"
                     /locus_tag="ACHY5W_001135"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFS4446070.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6615..9455)
                     /gene="glnE"
                     /locus_tag="ACHY5W_001136"
     CDS             complement(6615..9455)
                     /gene="glnE"
                     /locus_tag="ACHY5W_001136"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFS4446071.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(9478..10779)
                     /gene="ygiF"
                     /locus_tag="ACHY5W_001137"
     CDS             complement(9478..10779)
                     /gene="ygiF"
                     /locus_tag="ACHY5W_001137"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFS4446072.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSDPTLDLAQFPSEV
                     WPNGELPADLASRLQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            11021..11641
                     /gene="ygiM"
                     /locus_tag="ACHY5W_001138"
     CDS             11021..11641
                     /gene="ygiM"
                     /locus_tag="ACHY5W_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFS4446073.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11705..12943
                     /gene="cca"
                     /locus_tag="ACHY5W_001139"
     CDS             11705..12943
                     /gene="cca"
                     /locus_tag="ACHY5W_001139"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFS4446074.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYHQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(13124..13945)
                     /gene="bacA"
                     /locus_tag="ACHY5W_001140"
     CDS             complement(13124..13945)
                     /gene="bacA"
                     /locus_tag="ACHY5W_001140"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4446075.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(14036..14404)
                     /gene="folB"
                     /locus_tag="ACHY5W_001141"
     CDS             complement(14036..14404)
                     /gene="folB"
                     /locus_tag="ACHY5W_001141"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFS4446076.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQAIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            14509..15126
                     /gene="plsY"
                     /locus_tag="ACHY5W_001142"
     CDS             14509..15126
                     /gene="plsY"
                     /locus_tag="ACHY5W_001142"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4446077.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLMSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(15139..16071)
                     /gene="ttdR"
                     /locus_tag="ACHY5W_001143"
                     /gene_synonym="dan"
     CDS             complement(15139..16071)
                     /gene="ttdR"
                     /locus_tag="ACHY5W_001143"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFS4446078.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            16278..17189
                     /gene="ttdA"
                     /locus_tag="ACHY5W_001144"
     CDS             16278..17189
                     /gene="ttdA"
                     /locus_tag="ACHY5W_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFS4446079.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTSKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            17186..17791
                     /gene="ttdB"
                     /locus_tag="ACHY5W_001145"
     CDS             17186..17791
                     /gene="ttdB"
                     /locus_tag="ACHY5W_001145"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFS4446080.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTQEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17840..19303
                     /gene="ttdT"
                     /locus_tag="ACHY5W_001146"
     CDS             17840..19303
                     /gene="ttdT"
                     /locus_tag="ACHY5W_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFS4446081.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPGLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(19346..20359)
                     /gene="tsaD"
                     /locus_tag="ACHY5W_001147"
     CDS             complement(19346..20359)
                     /gene="tsaD"
                     /locus_tag="ACHY5W_001147"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4446082.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20597..20812
                     /gene="rpsU"
                     /locus_tag="ACHY5W_001148"
     CDS             20597..20812
                     /gene="rpsU"
                     /locus_tag="ACHY5W_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4446083.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20923..22668
                     /gene="dnaG"
                     /locus_tag="ACHY5W_001149"
     CDS             20923..22668
                     /gene="dnaG"
                     /locus_tag="ACHY5W_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4446084.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            22863..24704
                     /gene="rpoD"
                     /locus_tag="ACHY5W_001150"
     CDS             22863..24704
                     /gene="rpoD"
                     /locus_tag="ACHY5W_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4446085.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24783..25289)
                     /gene="mug"
                     /locus_tag="ACHY5W_001151"
     CDS             complement(24783..25289)
                     /gene="mug"
                     /locus_tag="ACHY5W_001151"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFS4446086.1"
                     /translation="MVEDILVPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            25414..25489
                     /locus_tag="ACHY5W_001152"
     tRNA            25414..25489
                     /locus_tag="ACHY5W_001152"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25447..25449,aa:Ile,seq:cat)
     gene            complement(25589..26434)
                     /locus_tag="ACHY5W_001153"
     CDS             complement(25589..26434)
                     /locus_tag="ACHY5W_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4446087.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKSSLLQIDTLIRQ
                     LVEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDCEIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADGLADLERMLHLFSGKPLPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
     gene            complement(26519..26716)
                     /locus_tag="ACHY5W_001154"
     CDS             complement(26519..26716)
                     /locus_tag="ACHY5W_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS4446088.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRILRQLQFLQQKR"
     gene            complement(26736..27224)
                     /locus_tag="ACHY5W_001155"
     CDS             complement(26736..27224)
                     /locus_tag="ACHY5W_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4446089.1"
                     /translation="MNLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYS
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTGAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(27221..27601)
                     /locus_tag="ACHY5W_001156"
     CDS             complement(27221..27601)
                     /locus_tag="ACHY5W_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS4446090.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGVPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGRHPEEAKQ"
     gene            complement(27690..28058)
                     /locus_tag="ACHY5W_001157"
     CDS             complement(27690..28058)
                     /locus_tag="ACHY5W_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS4446091.1"
                     /translation="MSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     DDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVALYAKGLTCDAD
                     TLGSCGYVYLAVYPTPDMKK"
     gene            complement(28134..28355)
                     /locus_tag="ACHY5W_001158"
     CDS             complement(28134..28355)
                     /locus_tag="ACHY5W_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4446092.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(28418..28894)
                     /locus_tag="ACHY5W_001159"
     CDS             complement(28418..28894)
                     /locus_tag="ACHY5W_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS4446093.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLLTERLVQALALVDIRVPDHLIIGGSQVFSFAEHGLL"
     gene            complement(28910..29383)
                     /locus_tag="ACHY5W_001160"
     CDS             complement(28910..29383)
                     /locus_tag="ACHY5W_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4446094.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATLIPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWILFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(29477..29722)
                     /locus_tag="ACHY5W_001161"
     CDS             complement(29477..29722)
                     /locus_tag="ACHY5W_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446095.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGGRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWSSVCDRNGYAYGCPEFQPNIIHP"
     gene            complement(29722..30162)
                     /locus_tag="ACHY5W_001162"
                     /pseudo
     CDS             complement(29722..30162)
                     /locus_tag="ACHY5W_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(30281..32059)
                     /locus_tag="ACHY5W_001163"
     CDS             complement(30281..32059)
                     /locus_tag="ACHY5W_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFS4446096.1"
                     /translation="MRNARTILGIIRKRGAEGKSLDQIYRMLFNPELYIAAYAKIGSN
                     KGAMTKGVTDETVDGMSHKKIHDIISLLRNERYKFSPARRIYIKKKNGKMRPLGLPTW
                     SDKLLQEVMRMILEAYYEPQFSQFSHGFRPQRGCHTALTVIAKTWTASTWFIEGDIKG
                     CFDNIDHEILLHILREQIKDNRFIRLIEKLLYSGYMEEWKCHKTWSGTPQGGIISPLL
                     SNIYLNKLDQYVEHVLLPKYNKGKSRARNREYEVQNGIEHYNRKRGRLDISRAARKKK
                     QTLPSKDPFDPHYRRLRYVRYADDFLLGFSGPKSEAEEIKKEITLFLQNNLSLEVSQE
                     KTLITHGRTDKARFLGYDIQVGQCDTFRNRKKRRSVNGDIRLLVPADVVREHSKRYFL
                     KGRVRRRPELLEESDYTIVHNFQTRFRGLANYYQLACDRSKKLSRLKGIMEHSLMLTL
                     ANKHKTWVSRIYQKYGTRCMMTNGKSYKCLQIRIDRVNKAPLTATWGGISLARNKSYR
                     NIVLVDEKDLLFYGRVERLKAILASECAICGSTQNIQVHHIRALKDLAKRGRTPTASQ
                     RRMAARRRKTLVVCHQCHVAIHQGKL"
     gene            complement(32572..32952)
                     /locus_tag="ACHY5W_001164"
                     /pseudo
     CDS             complement(32572..32952)
                     /locus_tag="ACHY5W_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(33172..33582)
                     /locus_tag="ACHY5W_001165"
     CDS             complement(33172..33582)
                     /locus_tag="ACHY5W_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446097.1"
                     /translation="MKTIIKTLLVAVTVLFSVNVAAKTIKLPAGIKYVNSTDEFYCTE
                     IDGLHCQTKNLFKYKGNRYVFVIELGGAWCYDNPMSVLNLETWKAQRLEYNDKRLCSG
                     NNEPFFEIKNGVPTVGIIDTSEKPVIVAQDKLKI"
     gene            complement(33598..34275)
                     /locus_tag="ACHY5W_001166"
                     /pseudo
     CDS             complement(33598..34275)
                     /locus_tag="ACHY5W_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8298 a         9133 c         8597 g         8251 t
ORIGIN      
        1 tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa
       61 tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagc
      121 agggaaaaat aaaaaccgaa gcctgctaca acttcggttt cttgattaag gcagtaattt
      181 aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc
      241 aatagtcacg agcgccatat tgtgtttatt ggcaaactca atacactctg gtgcacgcgc
      301 catcgtgcca tcgtcattag tcagctcaca cagcacacca gccggtttaa agcctgccag
      361 cgtcatcaga tcaatagtcg cttcagtatg accgccacgc gtcagtactc cacctgcctg
      421 cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc
      481 aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag tcacaccttc
      541 agccgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat
      601 cattggcaga tcgagttgtt tacggcgatc ttcagtaatg cacaggcaaa caataccgct
      661 accgtggcga atggtcagcg ccatctgctc aacagtcatg gtttctgccg ggaagatcat
      721 atcaccttcg ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag
      781 cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg
      841 attcatggta aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga
      901 gtggcggcat atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc
      961 tcccatccgg actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt
     1021 cgcacccaaa gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg
     1081 ccccgccctg agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata
     1141 gcttcacata attctggttt atgacttacc cttatcgcac tacaatggca ctcaacacct
     1201 atcagtacag ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg
     1261 ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc
     1321 gccaaaccct acaagcgcag ctgacgcgtc tcgatctggt gagccgcgaa gaattcgacg
     1381 tacagacgca agtcctgtta cggactcgtg aaaaactggc gttactggag cagcgcatca
     1441 gtgaactgga ggcgcgcaat aattcggtag cagatcttca atctcctccg gctatcccgc
     1501 ctattgataa agcagaataa agcaacacaa aaacgggcca gacggcccgt aatatcagtg
     1561 ttctaattca agttctctgg tttgcatcat ctgttggcaa taccaggcat aacgtgcgca
     1621 aaaccaggtt ctatgttctt catccatgtt accagtaacg gccagaatat cgaccgggcg
     1681 accttctgcg tgcattctgg caaaactacg cgccaggaaa tcgaaattat ttaaagaatt
     1741 aagactatga tccataagca catcctccgg ttatcgcata ccttttaagg ccatatgctt
     1801 tttctgattt cagtattcct caatcagtgt agtgccttta aacataacgt gcgtaaatat
     1861 attgtccccg atcacacttt ttagtgaaaa ataaaaaacc atattaaaag actttctctt
     1921 ccagaacttc tctaacatgt gttattatta acaaagacaa atgtaagcaa ggaaactatc
     1981 ccaaagtaaa tattgcgtca ctaaatggac attggagtga catatgattt tattcgccga
     2041 ctataacacc ccttatcttt tcgctatttc ctttgtactg ttgatcggcc ttttagaaat
     2101 ttttgcgctt atctgcggtc acatgctctc cggtgcactt gatgcacatc ttgaccatta
     2161 tgattccata accacaggtc atattagcca ggcacttcat tatctcaata tcggaagatt
     2221 gccagctctc gtcgttcttt gcttactggc tggttttttg gtcttattgg catcctgttg
     2281 cagcatacct gcatcatggt ctggcaatca ccactctcga acctgtttgt ggttcctgtc
     2341 agtttactat ttacgattat tgcggtgcat tataccggta aaatagttgc cccctggatt
     2401 ccacgcgacc atagttcagc tattacagaa gaagaatata ttggtagcat ggcattaatt
     2461 accggtcatc aagccacttc aggtaatccc tgcgaaggaa aactcaccga tcaatttggc
     2521 caaatacatt atttactact ggagcctgaa gaaggaaaaa ttttcactaa aggagacaaa
     2581 gtgttaatta tttgccgact ttctgcaacg cgatatttgg cggaaaacaa tccctggccc
     2641 caaatattgt aatttattaa tcaaaaggaa atttaataat ggatgatatt gttaattctg
     2701 tgccctcctg gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta
     2761 ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt actggtttag
     2821 gtgggcaaaa agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa
     2881 tccccatcaa tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga
     2941 ttacgaaaga tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt
     3001 cagtagaagg gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag
     3061 acttacgtat gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa
     3121 tgaccatgca tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg
     3181 tagctgaaga tctgtcgaaa aacggcctgg aactggaaag cgtttcactt accaacttta
     3241 accagacctc gaaagaacat ttcaatccta acaatgcctt tgacgccgaa ggtttaacca
     3301 aactgactca ggagacagag cgccgtcgcc gcgagcgtaa cgaagttgaa caggatgtag
     3361 aagttgcggt gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac
     3421 aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga
     3481 atgcgaaaat tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc
     3541 tggctgaacg acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa
     3601 aagttgaagc tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa
     3661 tcgctaacca gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag
     3721 aagcgcgtgc taaccttgca ctcgcagaag cagtaagcgc gcaacaaaac gtagaaacca
     3781 ctcgccagac agccgaagcc gatcgtgcca agcaggtggc gctgattgcc gcagcacagg
     3841 atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag
     3901 agatgcaggc ggcggcgatc gttgagttag ccgaagctac gcgtaaaaaa ggcctggcgg
     3961 aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgac gaacaaacca
     4021 gccttaaatt caaactggcc ttgttgcagg cgctacctgc ggtaatagag aaatccgtgg
     4081 aaccaatgaa gtccattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggtg
     4141 gtactgcggg ggatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat
     4201 tatcagccgc actctcttac cgcacacagg caccactgat tgactccttg ctcaatgaaa
     4261 ttggcgtttc aggcggctca ctggctgcat tgacttcacc cttaacctca acaactcccg
     4321 tcgccgaaaa cgtagaataa attttcagct atgaacatcc ctggcacaaa aagccgggga
     4381 ttttttatat ctgcgttccg ctaaaaggtg caaatgatca ggctgttgca gcgttttgcg
     4441 tgactgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcgaggt ccggcgctca
     4501 tcaacaatcg gggagtcagc aaagggctga aacgggaaag cccctcccga ggaaggggcc
     4561 ttacagaagg aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta
     4621 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc
     4681 ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg
     4741 cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac
     4801 gttgcagcgt tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg
     4861 cggggtccgg cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc
     4921 tcccgaggaa ggggccttat agaaggaaag ggttatgatg aagctcgtca tcatactggt
     4981 tgtgttgtta ctgttaagtt tcccgactta ctaacaactc atcagagggg ggagaaatcc
     5041 tcccttaccc ttgttccttt actctaggtt gaaaaaacaa cagcgtcaat aggcctgcca
     5101 tgtacgaagc gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt
     5161 cttgataatg ttagtggtcg agcaaccgtc ttcaaagttg agcaccaaca cttcgccgcc
     5221 gttggcccag acttctttac tcccggcgat ctcttccggt ttgtagtcac cgcctttcac
     5281 cagcaggtct ggcaggatcc cggcgatcag acgctgcggc gtgtcttctt caaacgacac
     5341 tacccagtcg accgcttcca gtgcgcccag cacaatcata cgctgttcga gtgggtttac
     5401 cgggcgggaa tcccctttca gccgtttggt ggaggcatcg ctgttgacgg caacaatcaa
     5461 gcggtcaccc agcttgcggg catttgccag ataagagacg tgcccggcat gtaggatatc
     5521 gaagacaccg ttggtcatta ccactttttc accacgttta cgcgctgccg ctactgccag
     5581 cttcagttcc tcttcggtca tcacgccaaa gccggtatct gcgcgtccac gtacagcgtt
     5641 ttccagctcg atcggcgaaa ccgtggacgt ccccagtttg ccgaccacca caccagccgc
     5701 cgcattggca aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag
     5761 gacgccaatc accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgggttgg
     5821 catatgcagc ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac
     5881 taacagagcc gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc
     5941 ttcggtctta catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt
     6001 agcgccgcgg tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacacccgc
     6061 tttacgcgcc agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga
     6121 aagcaccagc gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg
     6181 atcaacacct tcgaaacctt cttcaaaatc cagacggatc agctgttggt tgcgggaaag
     6241 tacccgtaat ttggtgatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt
     6301 gacgtcggcc agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac
     6361 cagacgtgca ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg
     6421 gcgttcttcg atggtattca ctttaaccac gggcaccggc gcttccggcg agatacgact
     6481 ggtggggccg taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg
     6541 ttcaaactct ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc
     6601 gataatacca tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca
     6661 cgctctgcgg tgaagcaatc ctccgacaca tggcccggca attcctgtaa tgccagatga
     6721 tgaagttcat cgcgaagcgt agtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc
     6781 ataatgtcgt tttgcgccag tagttccaga atacgcacgt tgtctgacca gcgcgttaac
     6841 ttcggttttt catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta
     6901 attccccctt catcagcttt gatatcaaag cgatcgcgat gtttattgcc gagatgagcg
     6961 cgcattttct cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc
     7021 gtcataatct cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact
     7081 acacgcgcac gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatcg
     7141 gcaaatgctt ctgtggaggt aaccagcatt cccgccgccc cggacgggcg cagccgcgca
     7201 tccacttcgt acaaaatgcc ggaagaggtt cgcgtgctga acaggtgcat aatgcgttgc
     7261 gccagacgca gataaaactg tcgtccgtcg atttcccgct cgccgtcagt catcgcatcc
     7321 attgggcaat catggagaaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg
     7381 cccagcttgc cgtagccgac cactgcaaaa ccacgccctt cgcgttcgtt caggtgattc
     7441 ggcttaccgt agcgggcaac catttgaacc cacgcctgct gaacgacggc atctatcatg
     7501 gcttccgcca gccaggttaa gtgatcgctc actttcatca ccggtagcgt accggcgata
     7561 tccgctgcgg cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt
     7621 tgctcttcgt catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca
     7681 tcggtcgccg tcggctggta aagggtgttt ggatcgagca attcatccag caataatgga
     7741 taacgcgcca gctggctggc aatcatcggc gacgcggcac acagagaaat caaatgttta
     7801 agcgccgcgg ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccgcc
     7861 agcaaggcgg taatgcgcga cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt
     7921 agcagatgcg gcatcagatg gtcgagcacc tgacgtcctc gcggcccgat ggtgcgctta
     7981 tccagctctt tgcggaaatc ggcaatcagc gttagcacct gtttgcgatc attttcgcta
     8041 agatgcgcca gcactggcgt agtgtcatct tcctgcaacg catcctgcca cagctcacgc
     8101 cactgttccg acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta
     8161 aacacccggc gcacattggt catatgtgcg gtcagcgccc cggtcaattg cggccagtca
     8221 gcaaagtcca tcgcccacgc cagccgcgca cgattaagct catcagaagg aagcgtctgg
     8281 gtttgttcgt cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatacgcc
     8341 actcgcaatt gttcagcatc gttttcagaa agcagatgca gcgcggcaat gacgctgagc
     8401 gttggcagta aagagcgcga ttgcagcgac ggttcacgcc caccgcgaat gagctggaac
     8461 acctggacga taaattcaat ttcgcgaatg ccacccgcgc caagtttgat gttgtcggtc
     8521 aaaccacgtc gacgcacttc acgggcaatc atccctttca tgttgcgcag cgactgaatc
     8581 acgctgaaat cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta
     8641 gcatagacgc cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag
     8701 tctcgcccct gctcctggta ataatcctcc aacgcggcaa agctcagcac cagcgggcca
     8761 ctttcgccaa acggacgcag tcgcatatcc acgcgataga caaagccatc ctgcgttggt
     8821 tgatccagca ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc attatccagt
     8881 tcccggcgtc caccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatca
     8941 gaggagaaat tcagttcccc gccgcccagc ttacccatgc ctaaaatcag cagcggttgc
     9001 gcttcgccct gcgcattgca cggcgttccc cactcgcggc agcaggcgtc atatagccag
     9061 tcacgcgccg caacaatcag cgtctccgcc agatagctga gctgctgcaa tatgctctct
     9121 tcagtaacca gtgccagcgt ttgcgcccag gcgatacgta ccatgatgcg ccgacggaac
     9181 agccgcagct cgcgcattaa cccggcttcg tcactcacat taatgagcgc ctcctgcaac
     9241 catgcaacgt aatgctgcca ttcgtcggcc tgcggcggtt ggctttccag ttccgtcagc
     9301 cactctggat gcgcaatcac gctgtcctgc acaaagtcac tgaaagtaag tactgacttc
     9361 gcctgtgcgc taagtgattc ctcggctaaa ggctctggca gccgctcaac aacggtctgc
     9421 cagtactgct gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta
     9481 acgttttccg ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc
     9541 aatttcgatg cgttgcccgg tcgcaatagc gtgatgcagc ccctgccagt tctccagcca
     9601 ggcttgcgcg acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg
     9661 cgtcaggcgt ggcaactggt cacggtagcg atcgcctaac ggctggcaga aaacgctttt
     9721 cagttcagcg gcatggcggg aaagatggat atcggcaaag cgtttgaagg agtcgctgat
     9781 tttgccctgc gctttggcat ctaaaaatgg ctgccatgct ttgcttacca gccattcggt
     9841 caacgccagc ttcgccattg ccgtttcggt agagtagacc gccgtcacgg cagaaaccgc
     9901 agaagcgatg gtcgcttcgc attgagtcag caaatcacgt aagtgagtgc tcgctttacg
     9961 cggcacaata ccaccaaaca gcatcagcgt atgacggacc aggccaatgg ctgccagcac
    10021 ctgttctttc gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc
    10081 taacgccagc tcgagcgccg cttccagccc ctgctcgaca tccgcttttg ccgcaacatg
    10141 taaaatggtg gtcggtttga tttcacgcgc cggattgccc tgtgccagat gatagccacg
    10201 cgccgctttg ctcagactgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag
    10261 tttcagcacc gcacgcatgt cgccgctaag cagctccagt tccagctcac agataggttc
    10321 agcaaattca cccgctttca cctccccctg atcgagagcg atttcaattc ggcttccatc
    10381 gaccgccacc agccattttt cgcgataaaa atcggtgctg aacagcggtt gtaggcggga
    10441 agcgagatcg gcaggcaatt cgccgttcgg ccagacttcc gagggaaact gcgccaggtc
    10501 gagcgtcgga tcgctcaacg ccacgttgta ttccgggcgc tggtgtaagc cgcctgtcac
    10561 tcttcctgca actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc
    10621 catgtcgtgc ccacgcagcc agttatccgg cgtttcgtag taaatattca gcaactgcac
    10681 ggggtcatgg tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcact
    10741 gtgattaaca ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg
    10801 tcacataagt gacgacgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt
    10861 ttgcgccaaa ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca
    10921 agacctgaca cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca
    10981 ctactatcgt tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat
    11041 cggattaact ttactcgcac ttagcgcgac tgccgtctca cacgctgaag aaacgcgcta
    11101 tgtttctgac gaactgaata cctgggtccg tagcggtccg ggtgatcatt atcgcctcgt
    11161 gggcacggtt aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta
    11221 tgcccaggtg aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac
    11281 tgagccaagc ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga
    11341 taaactcacc aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt
    11401 ggcgcagagc gacagcgtga tcaacgggtt aaaagaagaa aatcagaaac tgaaaaacga
    11461 gctgattgtc gcacagaaaa aggtcgatgc cgccagcgta cagctggatg acaaacagcg
    11521 caccatcatc atgcaatggt ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct
    11581 cggtctggta ctgccgcacc tgatcccaag ccgcaaacgc aaagatcgct ggatgaacta
    11641 aatcgccttc ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga
    11701 tggcgtgaag atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt
    11761 caaagacaga gattgggtgg tggtcggcag tacgccacag gagatgctcg acgcgggcta
    11821 ccaccaggta ggccgcgatt ttcctgtgtt tctgcatccg caaacacatg aagagtatgc
    11881 gctggcacgt accgaacgga aatccggttc cggttacacc ggttttacct gctatgccgc
    11941 accggatgtc acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc
    12001 ccaggacgat aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg
    12061 tctgttgcgc catgtttccc ccgcttttgg cgaagatccg ttacgcgtac tgcgcgtggc
    12121 gcgttttgct gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct
    12181 gatgcgcgag atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa
    12241 agagacggaa agcgccctca ccacccgcaa tccccaggtg ttcttccagg tactgcgcga
    12301 ttgcggcgca cttcgcgttt tattcccgga aattgacgcc ctgtttggcg ttccggcccc
    12361 tgccaggtgg catccggaaa tcgacacggg tattcatacc ttaatgacgc tctcaatggc
    12421 ggcgatgctg agtccgcagg tcgatgtccg tttcgcaact ttatgccacg atctcggtaa
    12481 aggactgacg ccgccagaac tctggccgcg tcatcatggt catggcccgg cgggtgttaa
    12541 gttagtggaa caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag
    12601 actggtggct gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat
    12661 cgtcaaattg tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc
    12721 gctgaccagc gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca
    12781 aggccgctgg ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagccgtcgt
    12841 tgaagcggga tttaaaggtg tggaaattcg cgaggagctg acccgacggc ggattgcggc
    12901 ggtagccagc tggaaggaac aacgttgccc aaagcctgag tgagtattgg gcattttgcc
    12961 ggatgcggcg taaacgcctt atccggctac atgtcaacga catttgtagg cctgataaga
    13021 cgcgcaagcg tcgcatcagg cgttgattgc cggatgcggc gtgaacgcct tattcggcct
    13081 acgcaatcgt ggacaacgcc aggcatccga cactattcct caattaaaag aacacgacat
    13141 acaccgcagc cgccacaata aagcgataaa tggcaaacgg gataaacgaa atgcgcttaa
    13201 tcaattgcag gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa
    13261 cggcaaacat cgggatatcg ccgcttgtca ggaagcccca gcttttgtag agatcgagcg
    13321 ccgttgcgcc catcatcatc ggcaccgcca gcaggaacga aaactcggaa gcagcgtaac
    13381 ggctcacccc catcagcatc ccacctgaaa tggtcgcccc ggaacgggag aaacccggcc
    13441 acagcgccag acactggaag cagccaatca taaacgcctg acgataggtc atatcatcca
    13501 gacccggtgc gcgcggttct ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc
    13561 caacgaccag cgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga
    13621 acaacagccc cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac
    13681 gacctttgct ttctccttcg tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa
    13741 acagacgccg ccagaacatc actactaccg ccagaattga tcctaactgg atcacgactt
    13801 caaaggtttt cgccgtgtcg ccctcaaacc ccaacaagtg accgacaata atcatatggc
    13861 ccgtgctgga taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta
    13921 tcagcagcga gtgcatatcg ctcatcaata aacccctaaa ttataaaaat gtaacgcttg
    13981 tccgaactac tgcagtatga ccaggttata accgtttggt ttaacagctg taaaattaat
    14041 tattttcttt cagattattg ccacgctcaa tgattacgcc aacattcgcc gcccgcgcca
    14101 ctgcgcctgg cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca
    14161 gctctgccac ctcttccgcc acgcgctcca ccagcgcaaa acgcgccccc tcgacgtggc
    14221 tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc cgccacatca tcacttttcg
    14281 ccgctttacg gttatcccac gccatttcga tatcgaacac taacttctgt tcgatggcct
    14341 gttcccagtc gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat
    14401 ccatcacgtc ctgcctgctt tttggctaac cggataccac ttccggcgaa atgtgcgtat
    14461 tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg aacagcttat gagtgcaatc
    14521 gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca tttccagtgc cattctggtt
    14581 tgccgcttgt gtgggctgcc cgatccgcga accagcggct ccggcaatcc cggcgcaacc
    14641 aatgtgttac gtatcggtgg caagggagca gccgtagcag tactgatttt cgacgttctg
    14701 aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg tcagcccctt ctggctaggc
    14761 ttaattgcca tcgccgcctg tcttggacac atctggcccg ttttcttcgg ttttaaagga
    14821 ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa ttggctggga tctcaccgga
    14881 gtaatggcgg gaacctggtt actgaccgtg ctaatgagcg gatactcgtc gctgggagcg
    14941 attgtcagtg cactgattgc tccgttttat gtctggtggt ttaagccaca attcaccttc
    15001 ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc atgacaacat ccagcgtctg
    15061 tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa gaaagcgcga aaaggatccc
    15121 gagtgatttc tggtggatct acatgacctg atagccttca tcgggcttgc ccagccgttg
    15181 ctggcaccat gccgccagaa attccacgca gacacgtaat ttcatgctgc gatagagcgg
    15241 ctcccggtaa acagcccaga tattggcgct ttgtgcatac tccggcaata cttgcaccag
    15301 tttgccactc tccagaaacg gcaacacatc ccactcggaa cgcagcataa tccctttgcc
    15361 ctccagcgcc cattgcagca caatctcgcc gctattggag gaaagatgcc cgcttacctt
    15421 caccgatttt ttctcctgac cgttccccaa ctcccatatt ccgtgggtca tatcgcgctc
    15481 tttggtcacc agacagtcat gacgacttaa ttcctgtagg gattgtggct gcggatattt
    15541 ttgcagatac tcaggtgctg cgcataatat tcttttattt tttgtcaaaa gatgcgcaat
    15601 ataataatca ggaatttcgt cattaatacg aatatccaga tcaatattat cctgcactaa
    15661 atcaatttgt cgatcgaaca attcaaaatg cacctgtaac tcaggataat tgcgcatcag
    15721 ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca aaactgcaac caatacgaat
    15781 catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct gatattgggt
    15841 gaggatttcc agcgcatgtt cgtaacaacg ttggccactt tcagtcagcg ccacgccgcg
    15901 agcggatcgg ttcagcagcg ttgttgccag cgtattttca aggatttgga tccgcttggt
    15961 gacgaatgca ggcgtttgcc caagcgtcgc cgctgcggcg ctaaaactcc cgctgtggac
    16021 gatttctacc agtacctgca aatctttggc taaaggccag ctattcagca tcgttaatta
    16081 tccgcagttg tgataagcgc agtgtatttc gcaaaacatt gccattccct gctctggcgc
    16141 aatattcatt cttattttcg cgttgattca cgaactgtta attacatatt cacggtggca
    16201 aaaaatataa aaccacattt ttagtggtag tttgtggcgg tgaatttttc cagacaaata
    16261 caaaaactgg agttgccatg atgagcgaaa gtaataagca acaggcagtg aataagttga
    16321 cggagattgt cgctaacttt accgccatga tttctacccg aatgcctgat gacgtggtgg
    16381 ataaactaaa acagctaaaa gatgccgaaa cgtcatcgat ggggaaaatt atctaccata
    16441 cgatgttcga caacatgcaa aaagcgattg acctgaatcg tcctgcctgt caggacaccg
    16501 gggagattat gttcttcgtt aaagtcggtt cccgcttccc actgcttggc gagctgcaaa
    16561 gcatactcaa acaagccgtg gaagaggcaa ccgtcaaagc gccactacgt cacaatgcgg
    16621 tagaaatttt tgacgaagta aacaccagca aaaataccgg cagcggcgta ccgtgggtca
    16681 cctgggatat cgtccccgac ggtgacgatg cggaaatcga agtttacatg gcaggcggcg
    16741 gctgcacgct accaggccgc tcgaaagtgt taatgccgtc agaaggctac gaaggcgtgg
    16801 tgaaattcgt cttcgaaaat atctccaccc tcgccgtaaa cgcctgtcca ccggtattgg
    16861 tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt actctcgcgt aaagccattt
    16921 tgcgcccgat tggcagccgc caccccaatc caaaagcggc agagctggag ctacgcctgg
    16981 aagaaggact caaccgtctg gggattggtc cacaagggct gactggcaac agttcagtga
    17041 tgggcgtgca tatcgaatct gccgctcgcc atccgtcaac catcggcgtt gctgtttcta
    17101 ccggctgctg ggcgcatcgt cgcggcacac tgctggttca tgccgatctc tcctttgaaa
    17161 atctgtctca cacccggagc gcgttatgaa aaagatcctg acaaccccga tcaaagctga
    17221 agatctgcaa gatattcgcg tcggcgatgt gatctacctg accggtacgc tggtgacctg
    17281 ccgcgacgtt tgtcaccgcc gcttgatcga actgaaacgt ccgatccctt acgatctcaa
    17341 cggcaaagcg attttccacg ctggccccat cgtgcgcaaa aacggcgaca aatgggagat
    17401 ggtttccgtc ggtccgacaa ccagtatgcg tatggaaagt tttgaacgtg aatttatcga
    17461 acagaccggc gtgaaactgg tggtcggcaa aggcggaatg gggccgctga cccaagaagg
    17521 ctgtcagaaa ttcaaggcgc tacatgtaat tttcccggca ggctgcgcgg tgctggcggc
    17581 aacccaggtg gaagagattg aggaggtgca ctggacagaa ctcgggatgc cggagtcact
    17641 gtgggtctgc cgggtcaaag agttcggccc gctgattgtc tctattgata cccacggcaa
    17701 caacctgata gcagaaaaca aaaagctgtt cgccgaacgc cgcgatccca tcgtggaaga
    17761 gatctgcgag cacgtccatt acatcaaata accctcccgg agaggctcgc cccctctcct
    17821 ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatacc ttgcgccgct
    17881 ggcggtcatc gccattattg ctctaattcc ggttcccgca gggctggaga gtcatacctg
    17941 gctctacttt gctgttttta ctggcgtgat cgttggactg atcctcgaac ccgtgccggg
    18001 tgccgtggtg gcgatggtgg gtatatccat catcgccata ctctctcccg ggctgctgtt
    18061 cagcccggag cagctcgctc agccaggctt taaattcact gcaaaatccc tctcgtgggc
    18121 cgtttccggt ttttctaatt cggttatctg gctgattttc gccgccttta tgtttggcac
    18181 aggctatgaa aaaaccgggc tggggcgacg tatcgcgctg attctggtga aaaagatggg
    18241 gcatcgcacg ctgtttcttg gctatgcggt gatgttctcc gagctgatcc tggcacctgt
    18301 cacaccgtcc aactcggcgc gtggtgcggg gattatctat cccatcatcc gtaacctgcc
    18361 gccgctctat caatcacaac caaacgacag cagttcacgc agcattggct cgtacatcat
    18421 gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg cgatggcacc
    18481 aaacttgctg ttaattggac tgatgaaaag tgcatctcac gccacgctga gttggggcga
    18541 ctggttcctc gggatgttgc cgctcagtat tttactggtg ctgctggttc cctggctggc
    18601 ttacgtgctg tacccgccgg tactgaagtc tggtgatcag gtaccgcgct gggcagagac
    18661 ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg ggctgatggt
    18721 aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga tggtcggtta
    18781 cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgata ttgtcagtaa
    18841 taaagcggcg tggaacgttt tcttctggct ggcctcgctt atcaccctcg cgaccggact
    18901 caacaacacc ggttttatta gctggtttgg caaactgtta gcaggcagct taagcggtta
    18961 ttcgccgacg atggtgatgg tggcgttgat tgtggtgttt tatctgctgc gctacttttt
    19021 tgccagcgcc acggcgtata cctccgctct cgcaccgatg atgattgccg ctgcgctggc
    19081 gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggtgcggcaa ttggtctggg
    19141 gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta gtggttatct
    19201 gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc tcgtattgct
    19261 ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtaagga cggtgccgga
    19321 agtaactctc cggcacccga tagttttacg cagccggtaa ctccgccagc ggccagcgcg
    19381 gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc attccggcat
    19441 aggcgatcat cgcaccatta tcagtacaaa attccggacg cgcgtagaac acttcgccgc
    19501 ggcgtttttt catcatttcc gccagtttcg cccgcagcgt gcggttagca ctcacgccgc
    19561 ccgccatgac cagtcgctta aagcccgtct gatccagtgc tcgcttacac ttaatcatca
    19621 acgtatcaac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc tggtcgtcgg
    19681 tgccgttgtc acgaatcgtg ttcgctgcga aggttttcag accagaaaag ctgaaatcca
    19741 gccccggacg gtcagtcatc gggcgtggga agacaaagcg cccggcagta ccctgagccg
    19801 ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc gcggttttat
    19861 caaacgcttc accagcagca tcatcgatag actcgccgag cagctcgtac tgaccaatgc
    19921 cagtcacgct gattaactgc gtatgaccgc cggaaaccag cagcgcgaca aacggaaatt
    19981 ccggtgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg tgtacaggga
    20041 tcgccggaac gttccaggca aacgccagag aacgccccac ggtcgcgcca accagcagtg
    20101 cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt gccgttaaac
    20161 cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga tcgcgggagg
    20221 ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta tacaattggt
    20281 tggctagcaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg caggaagttt
    20341 caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca gtgtaggacc
    20401 aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta tactcctcac
    20461 ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca gcaattgcag
    20521 taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa ccgaattaat
    20581 caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt tcgacgtagc
    20641 tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag ttcgtcgtcg
    20701 tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg cagtgaaacg
    20761 tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact aattccccga
    20821 gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc gaaaggaatg
    20881 cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg acgaatccca
    20941 cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat cgatgcccgt
    21001 gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca caacgagaaa
    21061 accccgtcct tcaccgttaa cggtgagaaa cagttttacc actgctttgg atgtggcgcg
    21121 cacggcaacg cgatcgactt cctgatgaac tacgacaagc ttgagttcgt cgaaacggtc
    21181 gaagagctgg cggcaatgca caatcttgaa gtgccatttg aagcaggcag cggccccagc
    21241 cagatcgagc gccatcagcg gcaaacgctt tatcagttga tggacggtct gaatacgttt
    21301 taccaacaat ctttacagca acctgttgcc acgtctgcgc gccagtatct ggaaaaacgc
    21361 ggattaagcc acgaggttat cgcccgcttt gcgattggtt ttgcgccccc cggctgggac
    21421 aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat tgatgcgggc
    21481 atgttggtca ctaacgatca ggggcgcagt tacgatcgtt tccgcgagcg ggtgatgttc
    21541 cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgtgtgct gggcaacgat
    21601 acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg ccagctttac
    21661 ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt ggtcgaaggc
    21721 tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc gtcgttaggt
    21781 acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa tgtcatttgc
    21841 tgttatgacg gcgaccgtgc aggccgtgat gccgcatggc gagcgctgga aacggcgctg
    21901 ccttacatga cagacggccg tcagctacgc tttatgtttt tgcctgatgg cgaagaccct
    21961 gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga gcaggcgatg
    22021 ccgctttccg catttctgtt taacagcctg atgccgcaag ttgatctgag tacccctgac
    22081 gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc gggcgaaacg
    22141 ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga tgacagccag
    22201 cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt tccgcagcta
    22261 aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga attagcgacg
    22321 ctggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg cttattcaga
    22381 gaactggtca acacttgtct ctcccagcca ggtctgacca ccgggcaact tttagagcac
    22441 tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga cgatatagca
    22501 gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt tgattcgctg
    22561 cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt aagcaacgaa
    22621 gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt taacggctta
    22681 agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc ggcaaatata
    22741 taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca gcgttaacgg
    22801 ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt ggataccgtc
    22861 ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt aaggagcaag
    22921 gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc gattcagatc
    22981 agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg gaagaagcac
    23041 cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct gccgaagccg
    23101 ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac ccggtacgca
    23161 tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa attgacatcg
    23221 ctaagcgtat tgaagacggg atcaaccagg ttcaatgctc cgttgctgaa tatccggaag
    23281 cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg cgtctgtccg
    23341 atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct accgccactc
    23401 acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa gacgaagaag
    23461 atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg gctcgcgaaa
    23521 aatttgcgga actgcgcgct cagtacgttg taacgcgtga caccatcaaa gcgaaaggtc
    23581 gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc aaacagttcc
    23641 gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg atggaccgcg
    23701 ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa atgccgaaga
    23761 aaaacttcat tactctgttt accggcaacg aaaccagcga tacctggttc aacgcggcaa
    23821 ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa gtgcatcgcg
    23881 ccctgcagaa actgcagcag attgaagaag aaaccggcct gaccatcgag caggttaaag
    23941 atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag aaagagatgg
    24001 ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac cgtggcttgc
    24061 agttccttga cctgattcag gaaggcaata tcggtctgat gaaagcggtt gataaattcg
    24121 aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt caggcgatca
    24181 cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg attgagacta
    24241 tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt gaaccgacgc
    24301 cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa gtgctgaaga
    24361 tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat tcgcatctgg
    24421 gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc accgaaagcc
    24481 tgcgtgcggc aacacacgac gtgctggctg gcctgaccgc gcgtgaagcg aaagttctgc
    24541 gtatgcgttt cggtatcgat atgaacaccg atcacacgct ggaagaagtg ggtaaacagt
    24601 tcgacgttac ccgtgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc aaactgcgtc
    24661 acccgagccg ttctgaagtg ctgcgtagct tcctggacga ttaatcggta ggccggctca
    24721 ggcgttacgc cgcacccggc acctgtccct ttgcacaaac gccacctttt cggtggcgtt
    24781 ttttatcgcc cacgcaccac cagcgcctgg tccagctcgc gatacgcttc aaccagtttc
    24841 tccagtgaaa cgcgacttaa accgctggga tttggcagca cccaaatctg cgtcgaacca
    24901 atgctgagcg tttgtttccc ccactgtgca ccgcgctggc tgaatccctg ttcatatgct
    24961 tgtttgccca gaatcgccaa cgcttgcggc tgataatctt caattttttc aatcagcttg
    25021 cgcccacctg cgtgtagctc ttgctttgaa atttcattgg cttgcaccgt tggacggtct
    25081 accagtttgg ttacgccaca acgataatcc agcagatgct gtgcctcctg cggcttcaac
    25141 tgacggtcgg taaacccggc ctgatatatc accttccaga agcgatttgc cggatgagca
    25201 aagggaaaac cagtcccggc ggatgaaagc ccagggttga taccgcaaaa cacgacccgt
    25261 aaccctggaa ccaaaatatc ctcaaccata tttactcctt tctaacatca ccaggaaaag
    25321 tataacggat tgaaaataca ttgtttataa aaacagcagg cgcgcggtaa tggctggatt
    25381 gcgacacgga gttactttat aatccgctac catggcccct tagctcagtg gttagagcag
    25441 gcgactcata atcgcttggt cgttggttca aacccaacag gggccaccat atacaacaaa
    25501 gggttagcta ataaatcagc taaccctttt tcttttacct ttgaattggg tcaggtaatg
    25561 ggtcaggtaa gctttttctc catctgactc agatggctga tagttgcccc ggataatgct
    25621 tcgcaatgat gtcatttagc cgatcaacca gttccggttt tctgaacgtg atgtgtgcgg
    25681 agcccttctt aaagtatctg atgctgaaca tctcatcttc atagtcctct ttaccctgaa
    25741 cagactgaat gtgatcatcc agacgaaggg tgatgttttc ccggttgtcg gggagcggtt
    25801 tgccgctgaa cagatgcagc attctttcca ggtcggcgag tccatctgcc tgttgcccgg
    25861 tgataaggtg aaatccccat ctgtcccacc tcaccaggtt gttgacgata attttactgc
    25921 caaatttaca gggggagttg gttttgtaat tccagctcag ccctctgaag acgttgatca
    25981 ctcctcgctc aaacacttca tctttattct ggtgcagttg ctcaaatgtg ctccaaatgt
    26041 tcgcttcact gatttccgga aaattatcgt actccagtga gcggtaccat gtatcacggg
    26101 cctgcgcatc cattaatgac agcatcccgg aacgttgcat caggtcacgc cagatttcgc
    26161 agtcaagatt gcgggtgatg gctttcattg cggtttccgg tttctccatg agccagcagc
    26221 cacagcgaaa atcctgtttc atggcccagt ccagagcagt tttacctcca atactttctg
    26281 tcagtaccga gatttctacc agttgtcgga taagtgtgtc gatttgcagc agtgaactct
    26341 ttcgtacagc cagaatgtgg ctgactgagg tggagcagat aacgtccgta tgcccgttga
    26401 ccacttccgg acgggagact gtgattgcag acataatcga tattcctttt cgcagataaa
    26461 tgaaaacgcc agccctgttc aggctggcgt cagtggaatg aagtatggtg agtgagactc
    26521 accgcttttg ttgcagaaac tgcagttggc gcaggatacg gatatcttcc ctggcctgtt
    26581 caatacaggg caacagtgct gccgaatccg ttttatcctc atcgttgtcg aagataatcc
    26641 ctgattcgca gtcaatattg tcctgcagcc acgcaatcag aatatccagc gccgtttctg
    26701 tggttaataa tttcatccag tgtcttttgt cctgattacc agtcgaaagt gggtaagccc
    26761 ggcaggcatt cagcgctggc atccagatga atgagactga cgccataacg ccgggtgaga
    26821 gtgacgacca gacgacggaa cgtttttgac aggcccaggc gcttaagccg cagaaccggg
    26881 tatgaccacg caccggtacg taacagatat ccgctacctg tgaaatgaat ccattctgat
    26941 tcgccggtgt cattcgtctg atgtgacagc gaatacaacc aggcgttgtc ctttgccgtg
    27001 atatgtgcgg tactgcagcg tatgccggtc agggcagcaa atggtggcgt gcgctctgct
    27061 gatgtccggt cagattcatc aacaatacga agggagtagc cattgtcgca ggccacgtta
    27121 atcagttcag cgagattaac accatcgacg tcaacgacaa tgcgtcccat gttcagcgcc
    27181 tgcacgttaa cgctgtctgc ttccagcgtc agggaaaggt tcattgtttc gcctcctccg
    27241 gatgcctgcc tagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc atcaggccgg
    27301 ttgccctgcg ggcccggagg atatcgatgc tgttgattaa ctgcgacggg actcctgcgc
    27361 tgaatcccgg ctggtcagtt cgtaccagcg cgtatttttc aacgagaaag ttcaccgcgt
    27421 cacacagtga aatccctgcc tcaatatgct gctcaatcac acgttcatca gagaacggtg
    27481 tgtcattcag cgtgaggcca tagtgctggt ccagcagtcg ggtgagcagt gtctgccaga
    27541 tttcaaccgg agacgggcag cgcgatgccg cccgtacgtg agtgtcgggt aatgttttca
    27601 tactgaagat tttcctgata tgcaaagata aaaatgggaa agtggcgtgg cgaaagcacc
    27661 aggccggagc agagggttat tccggtggtt tactttttca tatcgggcgt cggataaaca
    27721 gccagataaa cgtaaccaca ggagccgagg gtgtcggcat cgcaggtcag ccctttcgca
    27781 tacagtgcca cggtatgctg atggcgggga ttcagttcac cgctggtgag catgagttcc
    27841 agttgtttca tcagcagcgg aaaggcctgg tctaggtggt acgcatccgc gtcgctgaac
    27901 cggcctctga taccggcgcg gtcgtcaagg taatgcaacc ggttaccctc ctgcaccaga
    27961 cgtgccccga aacagggcgt cacggtgcag ggcagtcccc accaggggcg gtcgtgattg
    28021 tcgttgggat aatttgtctc atggagtgtg tctgacacga taaaatcctc acaaaaaatc
    28081 agttaagaat ggtccggtga tggcgataat tctgctcatc agaatgccct gactcagttc
    28141 agggtaacgc tcatcagccg gacatacggg ccaaaactgt ccttacggcg ttcagcaaac
    28201 acggccagca caccgggaat atcctgcacc tcacgaccgg tatatgcttc agtgctgccg
    28261 tgccagcggt atttgccggt acagaacgga aaaagacggg atgcaggatg ctgttggtga
    28321 atacgcatgg cttcaccacg ggtgatgatt ttcatggtgg gatacctctg aagacagaag
    28381 atagaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca tgttcggcaa
    28441 aggagaaaac ctggctgcca ccgattatca gatggtccgg cacccggatg tcaaccaggg
    28501 ccagtgcctg taccagacgt tcggtgagaa gccggtctgc cttactgggt gtgacttcac
    28561 cggacgggtg attgtgtgcc agcaccacgg cagcggcatt gtggtacagg gcgcgtttaa
    28621 tcacttcccg gggatggact tccgtgcggt taatggtgcc ggtgaagagg gtttcaccgg
    28681 caatcaactg attctggttg ttcagataca gcacccggaa ttcttcacgc tccagtcccg
    28741 ccatgttcag aatcagccat tcccgtgccg ctcgggtgga ggtgaaggcc acgccgggtt
    28801 catgaagatg gcggtccagg gttttcaggg cccgcagaat gagactgcgc tcgccgggcg
    28861 tcatctctcc gggcagaaag gaaagctgct gcattgtccc ggcctccatt cagtcgatga
    28921 tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcaac cggtaataat
    28981 ggaccgtcat ggcataacat tccgtacgac aggcatgatg gctgtacgtc atcagacagg
    29041 cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg gcattgaaca
    29101 gtatccaggt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcg ccaccgttgt
    29161 tcagggtgta cagattccag ataccaccgc agtagtcttc acacagacgg tccatccagc
    29221 cgaagacacg gggctccagg gtcacccact gtggaatgag gccaaaatgc tgcggccaga
    29281 agctgatgcg ctgttcatca gggatgaggg tggcaaccag ttgttgcagg ccagtctccg
    29341 gtgcacaggc tgaagaggtg gtgttctgag acaatgtttt catgagtgaa atccttgtaa
    29401 tgtgaataaa tgagagggag taaaggccgg gggctgacaa catcacgttc atcactgacc
    29461 gtgagccccg gtaatatcag ggatgaatga tattcggctg aaattccgga cagccgtacg
    29521 cgtaaccgtt ccggtcacag acggatgacc agatgccatt gcctgtactt agtctgcaca
    29581 gactgagttc tccgggacgg taaagcagac gggtacacca ggcgcacagg tcatcatcag
    29641 aaatggaacg ccctcccggc agcgcctctg gggcgtcggg tgattctggt acctgaatca
    29701 cgccttcagg gatatgtttc atcagcgcat actctccagc agcgtttctg ccatcaccca
    29761 caatgcgcgg ttgagcttaa tatcggtgtc gatgctgtga atggcccggg tatggatacg
    29821 ttttcctctg gcactgcgac cggaaatccc gcctttcagc atattctcct ggatggtctg
    29881 ataagcactc cacaggtcct taccgtaatc ctcccggcgt cgtggcgtca gaatgtcggc
    29941 ggtggtgacg ggctgatgtt cgtcaccata acggtaagtc agtgccgcct gtgccagcgc
    30001 ctggcgtgcc ggtggtggca ggaccagcga ctgcatggca tcacgtttct cttcaatccg
    30061 gtcaaaaacc cccaccacct cgtaagctcc ttcgataact ttctccacta catttccccg
    30121 gtgcggaaca cgcacttccc ccagagactg gccacagacg cacgtagggt aggctgccga
    30181 cgagttgctg tattggcact tttccgtcag cccccctccg aaccggacgt gcgactttca
    30241 tcgcatccgg ctctccagtt acaacaaatc cgcaaggtaa ttagagtttt ccctgatgaa
    30301 tcgcgacatg gcattggtga catacaacca atgtcttacg tcgccttgct gccatccttc
    30361 tttgtgatgc tgtgggcgtc cttccccgtt tcgcaagatc tttcagtgcc cgaatgtggt
    30421 gaacctgtat attttgggtt gagccacata ttgcacattc agaagccagt atggctttca
    30481 gtcgctcaac gcgcccataa aagagcaagt ctttctcatc aactaacaca atatttcggt
    30541 aacttttgtt acgggccaga gatattccgc cccatgttgc agtcaatggt gctttattaa
    30601 ctctatctat tcttatctgt agacatttgt aagatttacc attagtcatc atgcatcgtg
    30661 ttccatattt ttgatagatc cggctaaccc acgttttatg tttgttagcc agagttaaca
    30721 ttagggaatg ttccatgatg cctttcagac gagataattt tttacttctg tcacaggcta
    30781 gttgataata gttagctaaa ccacgaaaac gggtctggaa attatgaacg atagtgtaat
    30841 cactctcctc cagtaattcc ggtcgtctcc ttactcgtcc tttaagaaag taacgtttgc
    30901 tgtgttctct cactacatcg gcgggaacaa gtagccggat gtcgccattg acactgcgtc
    30961 ttttctttct gttacggaac gtatcacatt gaccaacctg aatatcgtat ccaagaaaac
    31021 gggctttatc tgttcttcca tgggtaataa gcgttttctc ctgagaaact tcaagactca
    31081 gattattctg aagaaacagg gttatttcct ttttgatttc ctcagcttct gattttgggc
    31141 cagagaatcc gagcaggaag tcgtctgcat atctcacata tcgcaaccgc cgataatgcg
    31201 ggtcaaaagg gtcttttgac ggcaacgttt gtttcttctt acgtgccgct ctggatatat
    31261 ccagtctccc ccttttacga ttgtaatgtt caataccatt ctgaacttca tactctcgat
    31321 tccttgctct ggatttaccc ttgttatatt taggcaataa cacgtgttcc acgtactggt
    31381 caagtttatt caggtatata ttagagagca acgggcttat tattccaccc tgaggtgttc
    31441 cactccaggt tttatggcat ttccattcct ccatatatcc ggagtaaagc agtttctcaa
    31501 ttaaccggat gaatcgatta tctttaattt gttcccgaag tatatgcagc aatatttcgt
    31561 gatcaatatt atcgaaacac cctttaatat ccccttcaat aaaccatgtg gaagccgtcc
    31621 atgttttggc gatgactgtc agtgctgtat gacaaccacg ctgtgggcga aaaccgtgag
    31681 agaactggct gaactgaggc tcgtaatagg cctcaaggat catacgcatg acctcctgga
    31741 gcagcttatc actccatgtt ggcaggccaa gaggacgcat tttcccgttc tttttcttaa
    31801 tatagatcct tctggctggg gagaacttat atctctcatt acgcagaaga ctgataatgt
    31861 catggatctt tttgtgcgac attccatcaa cggtttcgtc ggtaactcct tttgtcattg
    31921 cccctttgtt gctaccgatt tttgcgtagg cagcaatata taactccggg ttaaaaagca
    31981 tccgataaat ctgatccagc gatttacctt cagcccctct cttgcggatg atgcctaata
    32041 ttgttctggc gtttcgcatc tcgcgtacct ctcaatttta aacatcgttg taacctgcca
    32101 cccttcgcca tgtatccggc tttcccggac ccggactact acggtggctc cgttgcccta
    32161 gggctcgcgc cccttagaca atcccgtgtt tacacggtaa ggaacgtaac agcgcaactt
    32221 aggtgcccta ctcataacct tggtaccctc actgggtacg gcttaaccgg ggaatgattg
    32281 ggacgaccaa cggcatttcg tctgatccgg ttatcatcac tcttaaacgt ggtagtgacg
    32341 gggagtgcct tatcccgctt ggttattagg gttcaggcag tgtagctttc accatattgc
    32401 gcgagtctcc cggaacagtt cagccataca cgttttctgg ccctctccgg tttaccgcca
    32461 tgctattgtc cccttgacct ttcagcctca ggtaaggcgt tgactcagtg gtatttcatt
    32521 ccgaccactc ctgattgaat caggaatccc ttaccacgcc gacacggcgc acccgttctg
    32581 gcagacgaac ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt catgagagtt
    32641 gagcagaata atttcaggga catgttctcc gtttatctct ccggcccgcc gcagacgcag
    32701 catgtgtttt gtgtatcccc gacggcccgg gtcgcgcaca cgggtctggc aggcgaagaa
    32761 tggctggaag ccttcccgct gcagactttc cagtacggtg atggtgggga tgtacgtata
    32821 gttctggctt cgggacgtgt gtttatcttc tccgaaaatc cccggcacat ggtgcatcag
    32881 ctcttcatgt gtcagcggac ggtcacggcg tatctggttt gcagcataac caaaacgact
    32941 ggctaatcgc atactttgct ccttatcggt ggttaagatt tactggtgta ataaatgaaa
    33001 aagccacgtc tcccggagaa gacgcggcct gatgggtgaa atgaatgacg tttattgtct
    33061 gggaagccct tcactggcga gccacgcatt aagcggcttt ccggtaacaa tggctggggc
    33121 tgtatttacc tttgatatta ataatgtgca gagaaacgca tggtttactc cttagatttt
    33181 aagtttgtcc tgagctacaa tgacaggttt ttcagaggtg tcgataatac ctacagtcgg
    33241 gacaccattt ttgatctcga agaacggttc gttattacct gaacatagcc gcttgtcatt
    33301 gtattcaagg cgctgtgctt tccaagtttc caggttgagt accgacatag ggttgtcgta
    33361 gcaccaggca ccacctagtt caataacgaa aacatatctg ttacctttgt atttaaacaa
    33421 attctttgtc tggcaatgca aaccgtcgat ttctgtgcag tagaattcat cggtactgtt
    33481 gacgtatttg ataccagccg gtagctttat tgtttttgct gctacgttaa cagagaacag
    33541 aacggttaca gcaacgagta aagttttgat aatcgttttc atgatagact ccttaaatta
    33601 gttaaccgca gctacgtagt ttttcaccag atacgcgaat tcgtggacac caatttcacc
    33661 aaaccaaatc aggtaacctg ggtcgatgtt gtaatagtag ccaacgactc tatgcgtttt
    33721 tgggttatag agtgttacga taaagtccca gccgcaggcg tgggccttac aaccgccacc
    33781 ttctacccag gtcgttccgt taatgtttac aggatacgca ctaccatcag gaccatccat
    33841 atagccgtct tacccaaaca ttccgtttaa cccaacaggg gttaatgctt tgtcgatagc
    33901 agctctgtag gtagcgtcac cttcgtataa ctcgtacgtt gttggacacg ctccaggttg
    33961 ttcagcccag gctcccggct tacaactaag tgcttgtttt acggtggccg cttctgcgtt
    34021 aaagttaaag cccgtgaaca gaaccgccag aaataacatc agggtatgta aacgtttcat
    34081 tgtaaatatt cttccgttgt aaatggcact ggaaaatatc tcatcagtga aaccttccgg
    34141 aagtttccac cccttgtatg atgtatatcg ccagtatttt atttcatccc ggctgtaatt
    34201 tttatattcc ccggcacgtg caataacgct gaccagagat ataaggctca ttaataactt
    34261 tacaggtaat ttcataatt
//
LOCUS       DAZUGK010000027        34232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZUGK010000027 DAZUGK010000000
VERSION     DAZUGK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.027"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1091
                     /locus_tag="ACHY5W_001167"
     CDS             <1..1091
                     /locus_tag="ACHY5W_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS4446098.1"
                     /translation="FADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLG
                     IMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIILESLQPYLV
                     INKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNY
                     MQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPITVSID
                     EMTNALDPVELAAIINKKHHTIINGKKFKTSRAISCRSFNRYFTVSTDYRPKLEALLA
                     CSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEI
                     TTPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            1281..1394
                     /gene="yhiY"
                     /locus_tag="ACHY5W_001168"
     CDS             1281..1394
                     /gene="yhiY"
                     /locus_tag="ACHY5W_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFS4446099.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1457..2524
                     /gene="yhiI"
                     /locus_tag="ACHY5W_001169"
     CDS             1457..2524
                     /gene="yhiI"
                     /locus_tag="ACHY5W_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFS4446100.1"
                     /translation="MDKSKRHLAWWVVGLLAVVAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2521..5256
                     /gene="rbbA"
                     /locus_tag="ACHY5W_001170"
     CDS             2521..5256
                     /gene="rbbA"
                     /locus_tag="ACHY5W_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFS4446101.1"
                     /translation="MTHLELVPVPPFAQLAGVSQHYGKTVALNNITLDIPARSMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENTEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSVLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDMTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5256..6380
                     /gene="yhhJ"
                     /locus_tag="ACHY5W_001171"
     CDS             5256..6380
                     /gene="yhhJ"
                     /locus_tag="ACHY5W_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4446102.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6453..6728
                     /locus_tag="ACHY5W_001172"
     CDS             6453..6728
                     /locus_tag="ACHY5W_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4446103.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6725..7084
                     /locus_tag="ACHY5W_001173"
     CDS             6725..7084
                     /locus_tag="ACHY5W_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4446104.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7134..7994)
                     /locus_tag="ACHY5W_001174"
     CDS             complement(7134..7994)
                     /locus_tag="ACHY5W_001174"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFS4446105.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(8026..8295)
                     /locus_tag="ACHY5W_001175"
     CDS             complement(8026..8295)
                     /locus_tag="ACHY5W_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFS4446106.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEDE"
     gene            complement(8285..9793)
                     /locus_tag="ACHY5W_001176"
     CDS             complement(8285..9793)
                     /locus_tag="ACHY5W_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS4446107.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKIRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTIEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9786..11144)
                     /locus_tag="ACHY5W_001177"
     CDS             complement(9786..11144)
                     /locus_tag="ACHY5W_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS4446108.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(11221..11502)
                     /locus_tag="ACHY5W_001178"
     CDS             complement(11221..11502)
                     /locus_tag="ACHY5W_001178"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4446109.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(11499..11972)
                     /locus_tag="ACHY5W_001179"
     CDS             complement(11499..11972)
                     /locus_tag="ACHY5W_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4446110.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11997..12743)
                     /locus_tag="ACHY5W_001180"
     CDS             complement(11997..12743)
                     /locus_tag="ACHY5W_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4446111.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12942..13343)
                     /gene="nikR"
                     /locus_tag="ACHY5W_001181"
     CDS             complement(12942..13343)
                     /gene="nikR"
                     /locus_tag="ACHY5W_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFS4446112.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13349..14155)
                     /gene="nikE"
                     /locus_tag="ACHY5W_001182"
     CDS             complement(13349..14155)
                     /gene="nikE"
                     /locus_tag="ACHY5W_001182"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFS4446113.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLRAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALEVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(14152..14916)
                     /gene="nikD"
                     /locus_tag="ACHY5W_001183"
     CDS             complement(14152..14916)
                     /gene="nikD"
                     /locus_tag="ACHY5W_001183"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFS4446114.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14916..15749)
                     /gene="nikC"
                     /locus_tag="ACHY5W_001184"
     CDS             complement(14916..15749)
                     /gene="nikC"
                     /locus_tag="ACHY5W_001184"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFS4446115.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15746..16690)
                     /gene="nikB"
                     /locus_tag="ACHY5W_001185"
     CDS             complement(15746..16690)
                     /gene="nikB"
                     /locus_tag="ACHY5W_001185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFS4446116.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16690..18264)
                     /gene="nikA"
                     /locus_tag="ACHY5W_001186"
     CDS             complement(16690..18264)
                     /gene="nikA"
                     /locus_tag="ACHY5W_001186"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4446117.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18375..18962)
                     /gene="acpT"
                     /locus_tag="ACHY5W_001187"
     CDS             complement(18375..18962)
                     /gene="acpT"
                     /locus_tag="ACHY5W_001187"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFS4446118.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18964..20193)
                     /locus_tag="ACHY5W_001188"
     CDS             complement(18964..20193)
                     /locus_tag="ACHY5W_001188"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFS4446119.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(20190..20921)
                     /locus_tag="ACHY5W_001189"
     CDS             complement(20190..20921)
                     /locus_tag="ACHY5W_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFS4446120.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20921..21385)
                     /locus_tag="ACHY5W_001190"
     CDS             complement(20921..21385)
                     /locus_tag="ACHY5W_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFS4446121.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRRQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21382..22551)
                     /locus_tag="ACHY5W_001191"
     CDS             complement(21382..22551)
                     /locus_tag="ACHY5W_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFS4446122.1"
                     /translation="MIYISAVGMINALGNNLDDIAANLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGRQRIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22553..23137)
                     /locus_tag="ACHY5W_001192"
     CDS             complement(22553..23137)
                     /locus_tag="ACHY5W_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFS4446123.1"
                     /translation="MIKPTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGSRITL
                     PAPGISPAVNSQQLLTGSFNGNTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(23134..25452)
                     /locus_tag="ACHY5W_001193"
     CDS             complement(23134..25452)
                     /locus_tag="ACHY5W_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFS4446124.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTQLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDHDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCMISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAIGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDFPQQTLRRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25421..26026)
                     /locus_tag="ACHY5W_001194"
     CDS             complement(25421..26026)
                     /locus_tag="ACHY5W_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFS4446125.1"
                     /translation="MKFLPLLALLISPFVSALSLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLITRDQGLLWDQTSPFPMQLLLDDKRMAQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(26023..26445)
                     /locus_tag="ACHY5W_001195"
     CDS             complement(26023..26445)
                     /locus_tag="ACHY5W_001195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4446126.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKS"
     gene            complement(26449..28125)
                     /locus_tag="ACHY5W_001196"
     CDS             complement(26449..28125)
                     /locus_tag="ACHY5W_001196"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4446127.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMYGLQAAKDAGFSHAVQVDADGQHAIED
                     IPKLLAVAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKDKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(28116..28469)
                     /locus_tag="ACHY5W_001197"
     CDS             complement(28116..28469)
                     /locus_tag="ACHY5W_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFS4446128.1"
                     /translation="MKPHEIERHQAQANHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALALLAPGWRFHSIQNIKFQSPLLPENRVTLTLNWQEDRQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28456..29817)
                     /locus_tag="ACHY5W_001198"
     CDS             complement(28456..29817)
                     /locus_tag="ACHY5W_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4446129.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLDESFWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFSAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQISPPPVQVLLSAGGELPWQDVQHTANWLRVWPDEIYGST
                     ETGVIAWRYRDQEQRRWQPFPGMQFRAEGNAFRLFSPLMTEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     ARLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29814..30395)
                     /locus_tag="ACHY5W_001199"
     CDS             complement(29814..30395)
                     /locus_tag="ACHY5W_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446130.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(30400..30651)
                     /locus_tag="ACHY5W_001200"
     CDS             complement(30400..30651)
                     /locus_tag="ACHY5W_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4446131.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30663..30920)
                     /locus_tag="ACHY5W_001201"
     CDS             complement(30663..30920)
                     /locus_tag="ACHY5W_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFS4446132.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30895..31716)
                     /locus_tag="ACHY5W_001202"
     CDS             complement(30895..31716)
                     /locus_tag="ACHY5W_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4446133.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(31713..32435)
                     /locus_tag="ACHY5W_001203"
     CDS             complement(31713..32435)
                     /locus_tag="ACHY5W_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFS4446134.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(32476..33534)
                     /locus_tag="ACHY5W_001204"
     CDS             complement(32476..33534)
                     /locus_tag="ACHY5W_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS4446135.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33603..33980)
                     /locus_tag="ACHY5W_001205"
     CDS             complement(33603..33980)
                     /locus_tag="ACHY5W_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446136.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIVQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         8036 a         9095 c         9305 g         7796 t
ORIGIN      
        1 agttcgctga cagaacctat ttctccaatt gttcacctgt tgatcccaca agtaccagac
       61 caacctattt tggtgatacc gatggctcag ttggtgcagt tttgtacgcg ctttttgcaa
      121 cgggacattt gggaatcatg gctgaaggag aaaatttcct tagccagttg cttaatatag
      181 aagatgaagt tctcaacgta ttgctaaggg aaaactttaa tgagcaactt gacactaatg
      241 ttaatacaat aatcagtatc ctgaacagaa gagacattat tttagaaagt cttcagcctt
      301 atttagttat taataaagac gcagtcacgc catgtacgtt tctgggcgac caaaccggcg
      361 atcgatttag caatatctgt ggcgatcaat ttatcatcga tttgttaaaa cgcataatga
      421 gtatcaatga aaatgtccat gtattagctg gaaatcatga aacgaattgc aatggtaact
      481 atatgcaaaa cttcacgcgc atgaaaccac ttgatgagga tacctacgac gggataaaag
      541 attatcctgt atgtttctat gactccaaat acaaaataat ggcaaatcat catgggataa
      601 cttttgatga tcagcgaaaa cgttacatta taggaccgat aacagtctct attgatgaaa
      661 tgaccaatgc tctcgatccg gtcgaattag cggcaattat taataaaaaa catcatacta
      721 taattaatgg taaaaagttt aaaactagcc gggctatctc ttgtcgatcc tttaatcgtt
      781 attttactgt ttcgacagat tacagaccta aacttgaagc actattagca tgctcacaaa
      841 tgttaggtat aaatcaagtt gttgcacata acggtaatgg tggaagagaa cgcataggtg
      901 aaacggggac agttttaggt cttaacgccc gtgacagtaa acatgctgga agaatgttca
      961 gcatgcataa ttgccaaatt aaccctggtg cggggcctga gataaccact ccctggaagt
     1021 cttaccagca tgaaaaaaac aaaaatggac ttatgccttt gattagaagg cgcacaatgt
     1081 tacagctctg aaccatagac aacatgaata ttaatgttac tatctggttg agactgttga
     1141 taaaacgtaa aaaggaatgc tctgttcctg caactgaaca ggcgtgaaac gcgatccaac
     1201 aggatggata ccgttttgtc agccagtttt atagatcaca cttatgagta tgcgtgtttc
     1261 atttacggcc aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc
     1321 cgttggcgct caatgctctg cgcgctggtc gtatttgccg tttccttctt atcccggatg
     1381 gaagaatccg ctgaacgtcg ttcggcggca gttgtaacac gcgtcgtttg cttacaggga
     1441 ttatggcggg tcgattatgg ataagagtaa gcgccatctg gcgtggtggg ttgtcgggtt
     1501 actggcggtg gttgctatcg tggcgtggtg gctgttgcgc ccggcaggtg tgccggaagg
     1561 ctttgccgtc agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc
     1621 cgggcgtatc gacaccattc tggtgaaaga aggccagttt gttcgcgaag gcgaagtgct
     1681 ggcgaagatg gatactcgcg tgttgcagga gcagagactg gaagccatcg cgcaaatcaa
     1741 agaggcacaa agcgccgttg ccgccgcgca ggctttgctg gagcaacggc aaagcgaaac
     1801 tcgcgccgca cagtcgctgg ttaatcaacg ccaggccgaa ctggactccg ttgccaaacg
     1861 tcatacgcgt tcccgctcgc tcgcccaacg aggggctatt tctgcgcaac agctggatga
     1921 cgatcgcgcc gccgcagaga gcgcccgagc tgcgctggaa tcagcaaaag cccaggtttc
     1981 ggcttctaaa gccgctatag aagcggcacg caccaatatc attcaggccc aaacccgcgt
     2041 cgaagccgca caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa
     2101 agccccgcgt gacggacgcg tgcagtatcg ggttgccgag ccaggggaag tgctggcggc
     2161 aggcggtcgg gtgctgaata tggtcgatct cagcgacgtc tatatgacct tcttcctgcc
     2221 aaccgaacag gcgggcacgc tgaaacttgg cggtgaagcc cggctgatcc tcgatgccgc
     2281 gccagatctg cgtattcctg caaccatcag ttttgtcgcc agtgtcgccc agttcacgcc
     2341 aaaaaccgtc gaaaccagcg atgaacggct gaaactgatg ttccgcgtca aagcgcgtat
     2401 cccaccggaa ttactccagc agcatctgga atatgtcaaa acgggcttgc cgggcgtagc
     2461 gtgggtgcgg gtgaatgaag aacttccgtg gcctgacgac ctcgtggtga ggttgccgca
     2521 atgacgcatc tggaactggt tcccgtcccg cctttcgcgc aactggcggg cgtgagccag
     2581 cattatggaa aaaccgttgc gctgaacaat atcacactcg atattccggc ccgcagtatg
     2641 gtcgggctga ttggtccgga tggcgtcggg aagtcgagtt tgttgtcgtt gatttccgga
     2701 gcccgcgtca ttgagcaggg caatgtgatg gtgctgggcg gcgatatgcg cgacccgaag
     2761 catcgccgcg acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaatctc
     2821 taccacacct tatcggtgta tgaaaatgtc gattttttcg ctcgcctgtt cggtcacgac
     2881 aaagcggagc gggaagtgcg aattaatgag ttgctgacca gcaccgggtt agcaccgttt
     2941 cgcgatcgtc cggcaggtaa actctccggc gggatgaagc aaaaacttgg gctgtgctgc
     3001 gcgttaatcc acgacccgga actgttgatc cttgatgagc caacaacggg ggttgacccg
     3061 ctctcccgcg cccagttctg ggatctgatc gacagtattc gccagcggca gagcaatatg
     3121 agcgttctgg tcgccaccgc ctacatggaa gaggcagaac gcttcgactg gctggtagcg
     3181 atgaatgccg gagaagtgct ggcaaccggc agcgccgaag agctgcggca gcaaacgcaa
     3241 agtgccacgc tggaagaggc gtttataaat ctgttaccgc aagcgcaacg ccaggcgcat
     3301 caggcggtag tgatcccacc atatcaacct gaaaacacag agattgccat cgaagcgcgc
     3361 gatctgacca tgcgttttgg ttccttcgtt gccgttgatc acgtcaattt ccgcattcca
     3421 cgcggggaga tttttggttt tcttggttcg aacggctgcg gtaaatccac caccatgaaa
     3481 atgcttaccg gattgctgcc cgccagcgaa ggtgaggcgt ggctgttcgg gcaaccggtt
     3541 gatccaaaag atatcgatac tcgccgtcgg gtgggctata tgtcgcaggc gttttcgctc
     3601 tataacgagc tcaccgtgcg gcaaaacctt gagttacatg cccgtttgtt tcacatcccg
     3661 gaagcggaaa ttcccgcaag agtggctgaa atgagcgagc gttttaagct caacgacgtt
     3721 gaagatgttc tgccggagtc attgccgctc ggcattcgcc agcggctttc gctggcggtg
     3781 gcggtgattc atcgcccgga gatgttaatc ctcgatgagc ctacttctgg cgtcgatccg
     3841 gtggcgaggg atatgttctg gcagttgatg gtcgatctct cgcgccagga caaagtgact
     3901 atcttcatct ccacccactt tatgaacgaa gcggaacgtt gcgaccgcat ctcgctgatg
     3961 catgccggaa aagtgctcgc cagcggtaca ccgcaggaac tggttgagaa acgcggagcc
     4021 gcaagtctgg aagaggcatt tatcgcctat ttgcaggaag cggcagggca gagcaacgaa
     4081 gccgaagcgc cgcccgtgat acacgacacc acccacgcgc cgcgccaggg atttagcctg
     4141 cgccgtctgt ttagctacag ccgccgcgaa gcgctggaac tgcgacgcga tccggtacgt
     4201 tcgacgctgg cgctgatggg aacagtgatc ctgatgctga taatgggtta cggcatcagt
     4261 atggatgtgg aaaacctgcg ctttgcggtg cttgaccgcg accagaccgt cagtagccag
     4321 gcgtggacgc tcaacctctc cggttcccgt tactttatcg aacagccgcc gcttaccagt
     4381 tatgacgagc ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcaat tgagatccct
     4441 ccaaatttcg ggcgtgatat cgcgcgtggt acgcctgtgg aactcggcgt ctggatcgac
     4501 ggagcgatgc ccagtcgtgc cgaaacggta aaaggttacg tgcaggcaat gcaccagagt
     4561 tggcttcagg atgtggcaag ccgacaatcc acacccgcca gccaaagcgt gctgatgaat
     4621 attgagacgc gctatcgcta taacccggac gtaaaaagcc tgccagcgat tgttccggcg
     4681 gtgatcccgc ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg
     4741 gaaaaagaac tagggtcgat tatcaacctt tacgtgactc ccaccacccg tagcgagttt
     4801 ttgcttggca aacagctgcc gtacatcgcg ctggggatgc tgaacttttt cctgctctgc
     4861 gccttgtcgg tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac gctcaccctg
     4921 gcggcgctgc tgtatatcat cattgccacc ggaatggggc tgctgatctc cacctttatg
     4981 aaaagccaga ttgccgccat tttcggaacg gcgattatca cgttgatccc ggcgacacag
     5041 ttttccggga tgatcgatcc ggtagcttcg ctggaagggc ctggacgttg gatcggcgag
     5101 gtttacccga ccagtcattt tctgactatc gcccgcggaa cgttctcgaa agcgctggat
     5161 atgactgatt tgtggcaact ttttatcccg ttgctgatag ccatcccgct ggtgatgggc
     5221 ttaagcatcc tgctgctgaa aaaacaggag ggatgatgcg ccatttacgc aatattttta
     5281 atctgggtat caaagagttg cgcagtctac tcggtgataa agcgatgctg acattgattg
     5341 tcttctcgtt tacggtgtcg gtgtattcgt cagcgaccgt gacgccagga tcgttaaacc
     5401 tcgcgccgat cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca
     5461 gcttctatcg cccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg
     5521 gactggacgc cgggcgttat acctttgcga taaatattcc gcctaatttt cagcgtgatg
     5581 tcctcgccgg acgccagccg gatattcagg tgaacgtcga tgccacgcgc atgagccagg
     5641 catttaccgg caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg
     5701 cgcgctaccg tgataacagc gaaccgttgg tatcgctgga aacccggatg cgttttaacc
     5761 cgaaccttga tcccgcgtgg tttggcgggg tgatggcgat catcaacaac attaccatgc
     5821 tggcgattgt attgaccgga tcggcgctga tccgcgagcg tgaacacggc acagtggaac
     5881 atttactggt gatgccgata acgccgtttg agatcatgat ggctaaggtc tggtcgatgg
     5941 ggctggtggt gctggtggta tcgggattat cgctggtgct gatggtgaaa ggcgtgctgg
     6001 gcgtaccgat tgaaggctcg atcccgctgt ttatgctggg cgtggcgctc agtctgtttg
     6061 ccaccacgtc aatcggcatt tttatgggga cgatagcgcg ttcaatgccg caactggggc
     6121 tgctggtgat tctggtgctg ctgccgctgc aaatgctttc cggtggatcc acgccgcgcg
     6181 aaagtatgcc gcagatggtg caggacatta tgctgaccat gccgacgaca cactttgtta
     6241 gcctcgcgca ggccattctc taccggggtg ccggattcga aatcgtctgg ccgcagttcc
     6301 tgacgctgat ggcaattggc ggcgcgtttt tcaccatcgc gctgctgcga ttcaggaaga
     6361 cgattgggac aatggcgtaa ggaagggaaa cttgacttca tactgtgatg tcatactatt
     6421 gatgtcataa agtgaagtca taaggacatg ccatgagaac caaggtacag gctttacgga
     6481 agaaacaaaa aaatacattg gatcaaatat ttaaaactcc tgttcctcaa gggatcaagt
     6541 ggtctgatat agagtcacag gttaaagcat taggcggaga aattaaggaa ggaagaggtt
     6601 cgcgttgtaa gttcatacta aatatgagcg ttgcgtgttt ccatcggcct catccgtcgc
     6661 cagataccga taaaggcgct gtagaaagcg tgcgtgactg gttactaagt ataggggtaa
     6721 aaccatgatc aaactgaaga cgccaaactc tatggagatt gccgggcaac ctgctgttat
     6781 cacttacgtg ccagagctga atgcatttcg tggcaaattc ctgggcttgt caggttattg
     6841 tgattttgtc tctgacagca ttcaggggct gcaaaaagaa ggggaacttt ccctgcgaga
     6901 gtatcttgag gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag aaaagattaa
     6961 gacctttacg ttacgctatc cagaatcgct gagtgaacgc cttaataatg cggctgcgca
     7021 gcaacaagtc tctgttaata cctatattat tgaaacactg aatgagcgtt tgaatcattt
     7081 ataacttata gcggcccaac aatttctgtt gcgccgctat atttttgtgg ctcttaagat
     7141 gcctttctaa ccaaccgatg cccctcattc atcatcagga ttttgctata cacatcttcc
     7201 tcaacggcgt tttttgctga tgccatgact cgtgccagat tcgcctcgtt gtgattattc
     7261 acatacgctt tgccgacggc ggtgataaac gctttgcgca gatcgctggc gatatttact
     7321 ttagcgacgc gataattaac aaagctgcgt aatatctccg gggcgatacc ggagccgccg
     7381 tgaataacca acggcaccgg acagacttcg gcaatgcgct tcagcagggg gatatcgata
     7441 cgcggtatat cgtccaggcc atgaacgttg ccaatagaaa ctgccagcat gtcgcaaccg
     7501 gtacgctcga caaaggtctt caccttttcc ggttccgttt tgcaatcggc ttcgctgacg
     7561 tgatcatctt ctttgccgag aatcgccccc agctccgctt caaccggtac gccataggat
     7621 ttacagaaat cgacagcttc ctgggtgaaa gcgatattct cttcaaaaga gaatgcggca
     7681 ccgtcaatca tgaccgaggt aaagcccgcg cgcaccgcct gtttaacatc ctcctgggtt
     7741 ttcccgtgat ccagatgaag actgaccgga acatccattt tatcggcgtg acgtttcacg
     7801 atttcatata tgtaatcgta tcccgacagt tgtgcattgg ttggcgcaat ctgaatgaag
     7861 ttgggtaatc ccgaacgctc aatagcgttt aaaattgaaa ttgtcgtctc cagattggtg
     7921 gtattaaatg cgcccgctaa tacccgtttt tcctgaatgc ggtcgagcag tatccgtcca
     7981 ttaactaatg gcatattgaa aacctcatct tttaatatca gttaattatt catcttcacc
     8041 gaagcggtcg cgaaataagt cggtgagagt ctgtattgcc tcgttttcat catcaccgtc
     8101 gcagcgtatt tccaccgttt ttccgccttt tattccggcg cgcataatat tcatcatgct
     8161 ggtggcatta atatcattac cctcatagtg cagcgtcact ttacagctat attttttggc
     8221 ggctttcgcc agacaggccg ccggacgtgc atgcaggcca gtactgtttc tgacctccac
     8281 ggttttattc aacatagtgt tcctcccgat gatgtaatgg catcacgcct tcttgttgta
     8341 atttcaactg ataccagctt ttccatttgt gctgataacg ctgtaattga tggtatctcg
     8401 cccgattcgg taacaaacgt tgggctatag cgactggcgg cggtggaata tccggtgcgg
     8461 cggttaataa tgctccgaca gaaaccgcct cgttaaatgc aattgcttca accggacgct
     8521 gtaaaatatc cgcctttaat tgcagccaaa gaggattatg ggttgccgga ccgatcactt
     8581 ttaacgcgct ggtgccaggc acgttaaaac aggcgaagca gttggcaaat tcaatggtta
     8641 atcccagtga tacgctggca attaacatgt cgatacttaa cgtatcgcca agcccataaa
     8701 ataatccacg ggtatggcga tttttatagg gcgaacccga accgcgaaga tgtggaataa
     8761 agacgggaat gtcatcgagc aaccaattcc ccgccagata atcagcatgt ccgcgagtaa
     8821 gtgatgcggc gatctcctca tcggttagcc ggaacgtgtt gcgcagccac tccagcgcga
     8881 aaccgcccac gggcagcgac gcaaacaggg tgaactggtt ggcaagtgag taacagccgt
     8941 ttgccagctt gtcacgctta gcctgttcat ccagcgtcgg ttgtgtatcc agttgcagaa
     9001 tgccttccgt ggtccccgtc gagttaagga tatcgcctgg catcatctgc aacgctcgcg
     9061 cacccaccat atggtcatgt ccggccagcg tcacgctgac cggttgcgag aacccaagtg
     9121 tcttgcaaag tgactcgctg acccatccgg cgtgctcgcc tggctgcacc aatggcgcaa
     9181 atgccgtaca gggaacgtgt aacaacgccg ccgctttcgc tgaccattcg cctttgcgca
     9241 cgtccagaca cagcgtgcgg ctggctaagg tgatatccgt gcgctgttcg ccagtcagcc
     9301 gccagagcag cacttccggc gcatgtagcc agcgcaggcc gcggcgatta cgcagcgggt
     9361 aatgttccag caaccagcgt attttgaagg cagagtaatt gctgtgtagt ggtagaccgc
     9421 agatgtcata aagtgccgca ctgtctgcct cgctaagcgt tgccagatac tcttcaccgc
     9481 gacggtcata ccatgccagc attggcgtca ggatctcgcc atgctcgtca aggaacacgc
     9541 ctgattcgcc aaaactggca atgctgatcc gtctgactgg cagcggcgaa gcggcgttca
     9601 gttgcgctat cgcctggcgg acctcctgcc agagggcgtc gatatcgaaa tcgacatcgc
     9661 cctgtgggga gatctgcttt gccgtcacga atttatgcgc gcccaacgtc gttgcgtcca
     9721 ggcaggaaaa gcaggtgact ttgcaattgg tggtgccaat atcgataacg atagctgcgc
     9781 tgttatcagg cataaaccgt gctccgcgtc attcggaaaa tgccatacag caccagggcg
     9841 ataaccggca ccagaccaat ggcgatgata ttgccgttga agatttccag cagccagaag
     9901 cggaacgggt taccgccatc aaggaaactg gaaacctggc cgctggagcc ttcggcaaat
     9961 ttgaagttaa cgtctttcgc catgccggta ataaacggtg cgattttagt cgcaatccac
    10021 aggtcggcaa taatcaccgg gatcgccgcc agaacggtgc ggaaaatatt gccacggctg
    10081 gctaaagcaa tcatcgacgc cattaccgcc aggttggcaa ggtcgccaag cggcaggact
    10141 tcattacctg ggaggacaaa cgcgataaag accgagattg gcgttagcag cagggcggtg
    10201 gagataatcg ccggattacc tacggcaacg gcgatatcca gtccgatata gagatcgtca
    10261 cggtcagggt atttggcatt gagatacttt ttaatggctt cagacagcgg taataaacct
    10321 tccatcagga tgcgaaccat gcgtggcagg atgaacatca caccgccaat gctgatgcca
    10381 agcagcaaca cttttttgaa atcgtatcct gcaatcacgc cgagcagtat gcccagaata
    10441 gtgccaacca tcatcggttc gccgaagatg ccaaactttt tctgtacggt ttccgggtcg
    10501 atatgaatac gattgaggcc ggggatatgg tcgatgattt tgtcgaccag cagaccgacc
    10561 gggaagaaca ccaccgaaga gagcgtcggc aatgagatcc cttccagtcc aaagtatttt
    10621 tgtaccagtg gcgcagacca gtcggcgagt ttaagtacta ccaccgcagc aatcgccgcc
    10681 gccagcaaac caaagaacag gctgccggtg ctgtaataaa ccaacgtacc agcaagggca
    10741 aaatgccagt agttccacat atctacgtca acggtacggg ttttatttaa cgccagcatc
    10801 gccacgttaa tcagaatggt caggggaata acaaacgggg caattggcga accccatgta
    10861 atagcggaaa gcggcggcca gcctaaatct accaccggta aattaattcc ggtgcgttca
    10921 accatcgcct gggccgctgg cccgacattg ctggtcagaa tatcgaaaat ggcataaata
    10981 ccgacaaaac caatgccgac tgttaatgac gaacgtaagg ctttagcggg ttttacttta
    11041 aaacagagag caagaataaa taacaccaac ggcaacatta ccgtcggccc aaatcccaat
    11101 atatattgca caatattata gagggtatgc gcgatatcgt tcataataaa cctctttttc
    11161 aggcgagagc gcacctcgcg ttagcgcctg atggagtggg ttgaaattaa ttattattaa
    11221 ttaattttta aggatcgaaa caatttgcgc cagtactttc tcttcgccaa taccggtaat
    11281 aatcggcaag ccgttaacta ccgggacgtc gagttcataa ggcactttag tcgttgagac
    11341 taccagtgct acgccatcag tatttccagg taaagaggca acgttggttt gggaataatt
    11401 gacgttaaga ccgtgctctt tacaatattc catgactttt tctgttaccg ccgtggatgt
    11461 ggcaataccc gtggcgcaga caaataaaat ggtttgactc atatttcacc tcgttctttc
    11521 attttgttta agattactgt gtgtatttct gttgtttcca tcgcaagcag ggcttgcata
    11581 aagggcgtat cctgaatcat ttccataatc cagcccaggg catttaattg tttattactt
    11641 tcgcttaagg cgagcaaaaa aatcaccctg acaggtacgg ggtcaggatc gccgcccata
    11701 tcggcaaact ctaccggttc cggcaggatg ccgacggcaa ttgcattgtg cctgacatgt
    11761 ttatggtccg tgtgcggaat agcgacgcac ggtggttcgg taggcagccc cgttgggtac
    11821 gtcgcctcac gtttcagtac gtgatcggca aaatcaggga cgaccagctc ggcggcaacc
    11881 aacggttgcg ctaactgacg gatgatctgt tcaacgctta accctgccgg aagtcgttga
    11941 atataatttt cctgaaaatg tatgccttgc attttattcc tcttaaaaaa gtgtacttaa
    12001 ctcacttccc gccgttgcag tacggtgcca agataatatt tatttccttt taaccagacg
    12061 ttgccaaact caatgggtaa tcctcgggag aaaaagacta actgctccag atgcaaaacg
    12121 ggtgcgtctt cactgatatc taaaaaatga ccacgttcat taccaattaa tcgcgcggca
    12181 tagcgacttt ccgagtaacg aatttttctt tgcgacaaac tttctattgt tggaaataaa
    12241 ttgtgttgat taaaatcgat ttccgcaata ccggggcata gctcaatatt gatacggttc
    12301 tctatcagca tcgctttttc atcaccaatt gagcgtaagc gttcaaggta aagaatatcc
    12361 tgaccgggcg ttattcttaa tttctctgcc acataacgat tagccggttc aatccgtgac
    12421 gtaataactt cagtggtaaa atgtattttc tggctttcca gcgattccgc gaatgacaat
    12481 aatccttcac ctaacggata ggccacgttc tcttttttga caaatgttcc cttgccctgg
    12541 acctgaatca acacgccttc ttttaccagt tgggcgaccg cttttttaac ggttccccga
    12601 ctgacttcca gcatatccat aatctggtat tccgaaggga tgcgatcgtc ttccaccaga
    12661 tctccggtat aaatactttc tcttatccac tccaccactt gttgatagag cggtatttgt
    12721 gacgacttac ttaatgattt catggcgcgc acctgaacat ttacagttgt ctatggacaa
    12781 tcatggacaa cttcaagcgt atagtaggtg aacaggcgaa aaagaggatc gaagcgtctc
    12841 acattttgag ggaccaaatc gtgatcaaaa aaatgtgatg gaagtcgaag tttggcagga
    12901 atttactgcg ccgtgaggaa ataacggcgc gttgggaggt gttaatcttc cttcggcaag
    12961 cactgcaaat gcccgtgccg cacgccgcgc tgggcgatga cgtcatcggc aaaatgctgc
    13021 acgtcaccca tgtcaccttt cagcacggcg atttccagac agtcgtcgtg gttgatgtgc
    13081 acatgcagcg tggcgacgga gaggtcgtgg tgatgatgct gggtggaaac aatacggctg
    13141 gctaagtcgc gtttttcgtg ttcatacaca tacgacagca ccgcgaaacc ttgtgtgccg
    13201 tgctgctggg tagcttcctg cgccagggca ctacgcagaa tatcgcggat agcttcggaa
    13261 cggttgttat aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca
    13321 agcgtgatgg tgactcgttg catttgcgtt aaaccttttc tgaggtgcga cggcgcacgg
    13381 ggaatgcggg taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaggttaatt
    13441 tatctcccac cgcctgggtt tcgacgattt gtccgttgtc catcaccatc actcgctggc
    13501 aaaagcgttc caccaggcgt aagtcgtggg tgatgaacag gcaggcagtg ccaaactgtt
    13561 gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca aggttagaaa
    13621 cggcttcatc cagaatcagt agtttcggtt cgacctccag tgcgcgagcc aggcagacac
    13681 gttggagctg gccgccgctt aactgcggtg ggcgcttgtc gagcacgctg tcgtcgagat
    13741 cgaccgcgcg caacatttcg ctggcgcgcg ccagttgttc ggattttttc agtgatagca
    13801 ggtggcgcat cggttcacgc aggatctcgc gcacggtttt gcgcggattc acggcgctga
    13861 tggagtcctg aaataccatc tgaatatcgc ggcggaacgc tttacgctgg gcgcgattga
    13921 gctttgccaa cgattcgccg cgccagctaa tattcccttg tgagggagac tctaagccta
    13981 ccagcagccg cgcgagggtg cttttcccgc agccgctgcg ccccagcagg gcgacggttt
    14041 cgccgctttt cagggtcagg gaaacgttat tcagcacagc ctggtgctga tgttttccgc
    14101 taaatccacc gtgcgcatag tgatgggaaa ggccgcagac gttaagtaag gtcatgatgc
    14161 cagctccata ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtta ccgcatgttt
    14221 gggggcgtta aacagcgttt cgacatcgcc ctgttcgaca atcttaccgt cagacatcac
    14281 cgccacgtca tccgccagac gcgccactac gcccatatca tgggtgacca gcagcatccc
    14341 cggtgcctgt ttttgcataa tgctttccag cagatcgagg atgcgcgcct gcgctactac
    14401 atcgaggtca gtggtcggtt catcggcgat gataaacggt gattcacaca gcaccgccat
    14461 cgcaatcatc atgcgctgca acatgccgcc gctcatctcg aacgggtaca gcttcagcac
    14521 gcgtgcggcg ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc
    14581 gggtttcccc agcgccaggc aggtttcacg cgcgtgggtg tgcatggtgc gtagcggatt
    14641 aaaggcgctg cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaagg
    14701 cgaaaccggt ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcag
    14761 aatgcccagc gtcgcggcgc aggtcagtga tttcccgctg ccgctaccgc cgactaacgc
    14821 tagcacacgc ccgcgtttaa gggttaacga tacgccgtgc accagcggct gcgcggcctg
    14881 tagcgcgata ttacgcagtt caatctgttg cggcattagt gtgcgtgctc cgtcaccaga
    14941 tgagggtcca gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata
    15001 aacagcgcca gccccggcca gaacatttgc agcggctggg tccagatata ctggcgcgcg
    15061 tcgttaatca tcacgcccca ttcggcggtc ggtgcggtca cgccgaggcc gaggaaggac
    15121 atccccgcga cgtgcagcat catatggccg atatccagcg ttgccagcac cagcagcgaa
    15181 gggatcaccg cacctgccag atgatcaaca aacactcgca catggcccgc gccagaaagc
    15241 cgtgatgcca gcacaaactc gcgctggcgc agtgaaatca ccaggctgcg caccatgcgt
    15301 gcataccacg cccagtgcga cagggcgatg gcgataatta cgttggtcag cccagtgccg
    15361 agcacgccaa ccataaagaa cgacagaatc gaggtcggga aggtcataaa catatcggcg
    15421 acgcgcatgg tagcctgatc aacgcgcccg ccaatcagcc cggcgctgcc gccaataacc
    15481 agccctaatg tcagcaccag cagcaggcag gccattaccg aaccgagcga cacgcgggtc
    15541 gctgccatca gccgcgagaa aatatcgcga cctaagtgat cggtgcccag ccagtgctgc
    15601 gcattcggcg aaagcaggcg cgacggcaaa tcaatcgcct gggggtcata cggcagccac
    15661 cactggctgg tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgtacc
    15721 gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg
    15781 ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca
    15841 tcatcagcgt aaagcactgg atcaccggat agtcacggtt aaaaatcgcc gacaccgcat
    15901 agcgcccgac gcccggccag gcaaagatgt tttcgataat catcgtcccg ccaatcagtt
    15961 cgccgatgtg catccccacg gcggtgatca tcggcagcga ggcattgcgc aggatgtggc
    16021 gacgttcggt ctgtttgtcg ttcaggccgc gcagacgcgc ccaggtgacg tgacgctgac
    16081 cggcgacgtc caacatactg gcgcgcagta aacgcgcgtt aatcgccagc gacataaagg
    16141 caatggaaac cgcaggcaaa atgatgtgct gccagccgcc gtaacccatc gcgggtagcc
    16201 attgcagata caccgaaaac gccatcacca gtaaaaacgc cagccagaag ttaggcatcg
    16261 acacgccaag aaacgcgatg aaacgtacgg cgaaatccgg cagacggtca cgatggcgcg
    16321 ccgcccagat accgagcggt acggaagtga gcagaattaa taccagcgcc gcacccgcca
    16381 gttccagcgt agcaggcagg aagttcagca tatcgtccag cactgggcgt tggctggcga
    16441 atgagatacc aaagtcaaga tgcagcgcct tccacaacca ggtaccgtac tggacgtaca
    16501 gcggctgatc cagtccgagc atggtgcggg tagaggccag catctccggc gtcggcggca
    16561 ggttagacag acgcaaataa tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa
    16621 aaatgatcac cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt
    16681 aacgcaacat taaggtttca ccggtttaat ctgttcgaac ggaatttcgg tggcgatcgg
    16741 cgcgtagggg atgttaccca gctccggttt tgataccacc atcattgaga tgtaactgat
    16801 aggcagataa accgcctcgt catgcagacg ggtcagaatg tcgcgataca gcgcctgacg
    16861 ttgcgtttcg tcatgtgtcg ccagcacttc gccgatctct ttatcaatca gcggcttgtc
    16921 ggctaatcct tgctgtgcct ggaagtcagc gtgcgacggt acgcgcattg aactcaggaa
    16981 ggcgtgtggg tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc
    17041 gcgctgacgc gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat
    17101 ctggcgcata tcagcctgaa tgatttccgc catcgattta cttaacgcat cggtgccgat
    17161 gaacgaaagt tcaatgtgca gcggctgacc atttttctcg cggatgtctt tgcccgcagg
    17221 cagcgtccaa ccggcttttt ccagcaacgc tttcgctttc tgcggatcgt actggcgcgg
    17281 tttcaggccg aggttggcgt agggcacaga aggggcaaac agggtgtcgg cgacctgctg
    17341 ggtgccatac aacgcgttat caatcagcga ttttttgttt accgcgtaat taagagcttc
    17401 acgtactgcc agctcattgg tgggggcttt ggcggtatta agcgccaaca tcacggtttc
    17461 gatcggctgt gacagttggg tatggtaagc cgggttctgg ctaaagcggg cgaaggtatc
    17521 gagcggtaat aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgctac
    17581 cgcgcgggtc gtcgggtccg ggatgacgtt aaaagtgatc ttcttaatcg ccggcttttc
    17641 gccccagtag ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgcaa
    17701 aacccacggc ccggtgccaa tcggcgcttt aattccgttc atggtttcat ggtttttaaa
    17761 ctgcgagggg gcgataaagc ggaaagggcg cggcagggcc agttcttgca ggaaaggata
    17821 gtaggcgctt ttcagggtga tttgcagctc tgttttactg agtgctttga catcaacaat
    17881 ctggtttgcc agctccagcc aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt
    17941 ttctgccgct gcctctgcat caaacggttc gccgttggag aatttcacgt catcacgcag
    18001 ggtgaaggtc caggttttac catcttctga atgagtccag ctttttgcca gccacgggat
    18061 caccgaacca tcagcctgat atttcaccag cggttcataa accatgctct gggcgaacat
    18121 ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtggt
    18181 aatttcatct ggcgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag
    18241 agtgcggcgg agtgtggaga gcatggtaac cccaatggat taaaatagat ggcggaaata
    18301 agtatgacga ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca
    18361 gtcgggtggg cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta
    18421 aagggggtgg gggtgcagat cgccaggctt aaattttcta gctgacaatg gctgactgac
    18481 agcgaggagt gataggtgct gtctacgctg acgatttgcc aggcgctgcc accacgctgt
    18541 ttaacgatgg cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga
    18601 tgcacggcgt ccatctcagc gtgttccccg aggctgaata cggcgttcgc cagccagcgc
    18661 cagttggcgc gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcactcaac
    18721 agcagagcga tatcgtcacc gctatggctt aagttgaacc atagcggcgt ttccggcgca
    18781 aatgcaggtt tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa
    18841 agcaatgcac gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt
    18901 aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac
    18961 atatcagggc caacgtttaa tgatgatgga tgtattaatg ccgccaaagg caaagttgtt
    19021 actctgcaaa aactcacaat caaccttgcg ggcttcatgc atgatgtaat ccagcgcgcc
    19081 acaattcggg tctggtttat tcaggtttag cgtaggcgca aaccaacctt cgcgcatcat
    19141 ttgcagactc atccaggctt cgagcgcgcc acaggcacca agcgtatgac caaaataact
    19201 tttcagcgag gagagcggca cattatcacc gtaaatggtt gctgtcgcca gactttctgc
    19261 catatcaccg cgatcggtcg ccgtaccatg cgctgaaata tagccgatat cttgtgcgct
    19321 taacccggcg attttcagcg attgctccat acaatactgc atggtttcgc gctgcggctg
    19381 ggtaatatgt gccgcgtcgc agttcgtcgc aaagccgacg atttcaccgt aaatggtcgc
    19441 gccgcgcgct ttggcgtgct ccagttcttc gagaatgagc gtacctgcgc cttcaccaat
    19501 caccagcccg tcgcggtttt catcgaacgg tgaaggtgtg gttttcggcg catcattgtg
    19561 ctggctggtg gcgaaaagcg tatcaaatac cgctgcttct gaaggacata actcttcggc
    19621 accaccagca accatcaccg tttgataacc gtggcgaatg gcttcccacg cataaccgat
    19681 tgcctggctg ccggaagtac aggcgctgga ggttgggatc acccgcccgc gcaggccgaa
    19741 aaagaggcca gtattaacgg cggtggtgtg cggcatcatc tgcacatagg tggtgccagt
    19801 gatgttattg gtgtgctttt ccgtcagcat ggtggcgaac tcgcttaccg ggccggtact
    19861 gccggtcgat gaaccgtacg caatgcccgt ttcgccgctg gttaatatcg gatcgccaat
    19921 caaccctgcc tgctctaacg ccagctcgct ggcacgggtc gacatttgcg acacgcggcc
    19981 catcgcacgg atacgcttgc gggtgtagtg ctccggtagc gtgaaatcat caacaggcgc
    20041 gccaagcagg gtgtgcaggc catcgtagac ctgccactcc ggcattttgc gcaccgcatt
    20101 ttcatacgcc agtaagcgtg cagaaacgtc ctgccagttt tcaccaaagg cagtcacgcc
    20161 gcccattcct gtaatcacta cgcgacgtgt cataacatcc ctccgttgat ggaaataacc
    20221 tggcgggtga cgtaacccgc aatatcagac attaaataac tggcaagccc ggcaacttct
    20281 tccgcctggc ccatacgttt catggggatc atcgacattg cctctttcag agcgctctct
    20341 tccatttcga tcatcccggt atcaataagc cccggcgcga tgcagttgac ggtgattttg
    20401 cgcttcgcga gttcgatagc cagcgctttt gttgcgccga taattccggc tttggcagcg
    20461 ctgtaattca cctggccgcg attgcccatt acgccggaaa ccgaggatag cgtgatgatg
    20521 cgcccaccct ggcgtgcgcc gatcatcggc ataatgcacg gctgaatgac gttgtagaaa
    20581 ctgtcgaggt tggtgtggat caccgcatcc cagtcgtcat cgcttaaagc cggaaaagcg
    20641 gcatcgcggg cgatcccggc gttactgacc acgccgtacc aggcaccgtg ttgcgcgatc
    20701 tcatgctcca gcacttcccg acactgttcg cggtttgcga cgtcgaaact gagcaaacgc
    20761 ccgttacccc cgttggcgac aatggcattc agcgtttcct gagcgcctgt ggcgtcacgg
    20821 tgatagtgaa cgccaatgtt aaagccgtct gccgccagtt ggcaggcgat ggcgcgacca
    20881 atgcctttgc tggcaccagt aaccagaact gaacgactca tcaggacgct ccttgttgaa
    20941 acagggtggt aagttcttcc gcggtcggct ggaaggtgtt aatgcggccc gtcgcctcgc
    21001 cgtcgttgat gctgcactca aaactgccga agcgatcgtc ttgcatcagc agtttgacgg
    21061 taatggtcag cgtctggcct gcgggcagaa tgccagtggt gcaaagcaat tcacgcgcgc
    21121 ccagtatcat ccctaactca atactgtttt tgccctgctg gcggcgatgc cagccagacc
    21181 agacaccgac ggtttgcgcc atcagttcaa gggcaaacca gccgggtaga ttgccgtccg
    21241 ggtcgaggaa cggtgccagt acgccgctgg gcgagaccgt tacgcggcac accgcgctgt
    21301 catcgctgac gctcaccacg tcttccagca gtaacatggg cgcgtcgtgg ggcagataag
    21361 cgccggggga taaatagtgg ctcacgaaac cctcccgagc agaatgctgg cgttattgcc
    21421 gccaaaggcg aaggaattgg acaggatcac cggacgtgcc agcggctgcg gtttttcgat
    21481 aatgccacac ggtggcagcg tgggatcgcg cggcgacagg ctgaagtcct gagcgggcag
    21541 cggtaaatca cgctgtaaaa tcaacatact aattgccgcc tcggtaatgc ccgctgcgcc
    21601 cagcgtgtgt ccggtcaaat gtttggtgga gctgcacggt acgcgttcgc caaacagggc
    21661 gttaaccacc attgattcaa tctgatcgtt gagttgtgtg gcggtgccgt gcaggttgat
    21721 atagccgacg tcatctggcg taagctgcgc atcggtcagc gcctgattaa tagcgcggat
    21781 cgctccttcg ccctgcggat gcggggctga aatatggtaa gcgtcgctcg attcaccaac
    21841 gcccagcaac gcgatgggct gcggttcccg ggtcagcagc attagccccg ccccttcacc
    21901 aatggtgata cctgcgcggt cacgaccaaa cggctgacat aaggttggcg acagcgattc
    21961 gaggctgtga aaaccgttaa tcggcatccg gctcagggtg tctgcgccac ccacaatggc
    22021 ggcatcgacc agccccgctt cgataagcct gcgcccgctg attatcgctc tggcgctgga
    22081 agagcaggcg gttgaaaggg tataggccgg gccgtcgagc gccagccagt ggctgagaaa
    22141 acgcgacgga tcgccgagtt cctgttgcgc gtagtgccat gtggtgctcg gctgcccgtg
    22201 ggtgcgcagg ttaacgtgcg tgtcgccttc atgcaggccg gaggtgctgg tgccaagtac
    22261 aatcgcgatg cgctggcgac catatttcgc gatagcgtca tcaacctgcg gctggagctg
    22321 cgccagcgcc gccagcagga tctgattgtt gcgcgagcga tgcgcagcga atttttccgg
    22381 gataagcgga agctcgccat ccacgccagc caggaccgcc tgcggatgcc cctgcaacca
    22441 gcctgcgcgt gggcgcattc cgggggcgac accgcgagtc aggttggctg cgatatcgtc
    22501 aaggttattt cccagggcgt tgatcatgcc gacggcggaa atataaatca tatcagtcac
    22561 ctaagtattg aatggtgatg tggtatttaa agacatgctg ctcaatgctg attggcacgc
    22621 gtttgccctt gcgattcaga taggttattt ccgtgaccag tttgccgctg gcgttacgca
    22681 gctcgcgttt gtcaccgttg tcgcgaagtg tccatcctgc gggaagttgc ggttgccagg
    22741 cgctaatcgg ccagtggctg agcatcacgt cagccagtac ctgacttgcg ggcggtaact
    22801 gtgggacgac gatggattgc tcggcgcgta gcccttgcgc atcgtaggtc accagaaaca
    22861 ggcgaatgcc gaccgacgac agtccggcaa gggtgatttt ctggtcgtcg gcgttaagca
    22921 tcactagcag cgactgggtg ttgccgttga agctgccggt caacagttgc tgggaattga
    22981 cggcgggtga aatccccggc gcaggcagcg tgatgcgcga gccaggttgc agccacgctt
    23041 gcgggcggcc ttcctgttct ggttgcgagt ggctacagcc ggtgaggata agcgtagcgg
    23101 tcagggcaaa cgctcgccag aatgtgggtt tgatcatttt tttgttctct ttttatcggg
    23161 catagccagc ggcgaaagca ggaaggcggt gaaaataccg ctcaccagca caatgccaaa
    23221 actgctgatg gcctgggtgg cgctgaatac caacattccc agcgtcagca aggtggtgag
    23281 cattgccagc gcgatcgcca gtagcgaagt taacggtgta ccgcgcggat tactgaaaaa
    23341 cagcgtgtag ttgataccga tgccaagcac cagcaccagc gccagcagcg aaaagagatt
    23401 caccgcctgc ccgctcatcg ccagcaccgc cagaccacag cccagcgaaa gcaccgaagg
    23461 caccaggctg ataagccctt tgcgccagcc gagacgggcc actgcgccgc aggcaatcac
    23521 tgccagcgcc accagcaaca agccggttaa gacgtagcgg taaagtgcga acaattcatc
    23581 aaaggtgctt ttgcgatcaa cccaggcaat gccgcaaggg taatatgtgg cgatttcctg
    23641 catcaacgcg ctacttttaa ccccttcaac cggcaccagt acgccgcttt cgccgttttc
    23701 cagcgtcagc cacagcagac gccagccttc actggcgggg cttgccagcc attcatcaac
    23761 gttcactggc atggcgttga gatccgggtt cactgccgtc agcccggcat tttgcagcgc
    23821 ttttgttact gtcggggccg ccgttttcag caggtctaaa tcttcctcct gccgcgccag
    23881 ggagttcagc ggaatggtgc ggtagttgct gataaggccg tcttttttcg ctttctccag
    23941 tgaggtggta aatgtctcca gccgccgcaa tgtttgctgt gggaagtcgc cgtaaactac
    24001 aaaccatttt tgatcgacgc tttgtccggt cagggcggta atggctttct cctgcgccag
    24061 aatatgctgc ggcagcgcct gtaactgcga gatatcgtca tcgacgcgca gcattgacat
    24121 ccccgccagc gaaaacagcg ccagcgcgac gggcagaccc agcgacagtt ttttattgcg
    24181 ccgccacgct gccagccagc gtagcatcag cgccatcgcc ggaaccgggc gcaccggcag
    24241 gccacggcac agccacggat gccagaacag gacggtcaga caagaggcgc tcaacccgat
    24301 ggcggcaaaa atcgccatct ggcggatacc ggggaagggg gcgagcatca taatcagata
    24361 tgccgccacg gtggtgagca gcgccagtag caaggcattg cgtactttcg ccaggctttg
    24421 ccacggcgaa acgtcgttgc cgtgcaccat ccgctcggtg agataataga gtgtgtagtc
    24481 agcggaaatg ccgataacgc tcatgctcat caccagcgtc atcaggtgta attcaccgaa
    24541 aatcaacaat gtggcgaccg ttcccgccag cgcgccgatg ccgatggaaa tcatgcaaag
    24601 cagcaacgga cgcaaagagc ggaacaccgc cacaatcagc aatatcaccc ccagcagcgt
    24661 tgccacgccc agggttgaga tatcctgctt cgcctgttga ctggcgtaat cgctgtaaaa
    24721 taccgtgccg cgcgagagca actgcgcctg tgggtaacgc gttttcagat ccttttccag
    24781 cgtattcagg gtcgtgatga gctggtgggt ttgctgcata tcaaacgacg atcccgccag
    24841 ttcgccgtgc agcagatacc agtagttgcc ctggggatcc tgcgtcacca gccagccgtc
    24901 catcagccgc aaacgctggc cattttttgc catcgccagc tgcgagccgc gcattaacat
    24961 cagcggatcg ttttgcagct ccttgccgct taccccggag aatgcagaat aaagctggga
    25021 gaggatccac tgcgcctgcg cttcgccgcc gttttgcagg cgggcgcggg tgtcgtggtc
    25081 aatcaggccg ttgcggtgct gccagaaaaa cgctccccac gcctgctggc tggcggcatc
    25141 cattggccct ttaatgtcgc caagcgcagc ggatttttgc agctgcgtca gccactcctg
    25201 agcgaccctg ggattagcct ctttaccggg gctgaccagc cacaccagtt ggcggtccag
    25261 acgctgcata aagccgtcat tcagcgctgg gggaatatcg cccatcgcct gtttgggtaa
    25321 catagccagc acgctactgt tcagccgtga ttgcggcagc aggatcagca acgccgccgc
    25381 catgaccagg cagactagcc cccataacag cgcggggcgt ttactgggcg gcaaaacgtt
    25441 ggcgttcgtc atcggtcagt tgcgctggtg tcagttgatg ttgggtaaga gcaatatcgg
    25501 tgcgatcgcc ctgtttatca ttaagctgaa tgctctccag ataggtttgc ccggcgagat
    25561 cgatggtgtt gaaaatttta tccagtggcg tggtggtcgg cgtcaggcgc agcgtccagc
    25621 ggccttcgcc tttgtcggca aattcgacgc ggaagttttg ttccagcact ttgcgatcgg
    25681 cctggaacag cgcgcgcagc aggtggttaa actggaacat ctgcgggtta ttttctgccg
    25741 tgatgatttg cggcggctga ccgttgatca cctgcgccat gcgtttatcg tccagtaata
    25801 gttgcatggg gaacggtgag gtttgatccc acaacagccc ctggtcgcgg gtgatcaaca
    25861 tctggccctg agatcgcagc ggctgcggca gatctttaat cgtccgggtt tgatcgaaat
    25921 gggcgcggat caccggctgt tcggtaaagc gttgctgaag atcgtccagg ctcagggcgc
    25981 tcacaaacgg gctaatcagc agcgccagca gcggtaaaaa tttcatgatt tgactcccat
    26041 gcgttcaaac agaatatcgg ggctgacaaa gcacagttcg cgactttgct cgtcgaccgc
    26101 gacctgaatg gtgtatccgg tggtggcgcg tttgcccgtt tcggcatcga aaatctgata
    26161 gccaatccgc agacggtttt cgaactcttc gatatgcgca cgcacgcgga tgcgttgctc
    26221 aaaggtgagg gcgtggcgat atttcacccg cgcatcgacc actggccaca aataaccgga
    26281 ctctttcatc tgccgatagc cgtaattgaa ctggttgagc agcgcctcgc gggccacttc
    26341 aaagtagcga aaatagttac cgtgccaggc cacgcccatc atatcgacgt cgtgaaaggg
    26401 aatggtcagc tcgacttcag cggtaaatcg gggatcgtta agcacccttt actccttgtc
    26461 ctgaatttcc ggcagttgcc agaaatcgaa aaaattaaac cagtcgaggg gcgactggag
    26521 cgcgtaatgt tccagacgcg cggcgtagtg gtcgatagcg ttttgcagcg cctgttggcg
    26581 ttcgccacgc ggcaacagca gcgggtcggc aaaggtttcg cagtgaatat gcagcttgcc
    26641 gtgctggcgt agggcgaaaa tcagattcac cgggcagcgc aaaatagacg cgagaataaa
    26701 tggcccctgt gggaaaggcg caggctggcc cataaacgga ctccagcaga cgcgccagtc
    26761 gccgccgcgt tgcgggttga cggcgatgcg gtcgccgaca atcgccaccc attcgccgtt
    26821 atccagctta tcttttagca ggatggcggt ttccgggccg atatctgtta ccggcatcag
    26881 gttgatacca gcctgcgggg ccatctcctg cattatctgt ttaaagcgtt gggcgttttc
    26941 gctaaacacc agcgcgttaa tggttttgta gccctgaatt tttgccagcg cccggcacac
    27001 ttccacatcg ccaagatgcg aggccagcag caatttgccg cgcggatcgc ggacgtcaag
    27061 cgccgcttct gcgcctggcg caaacagcac atcacgcccc agttgtagct cgccgcgcca
    27121 gctggcgatt ttgtcgagca tggcattacc gaaacgtaga aagtgctgat agctgttgag
    27181 ttttgccgcc tgtggttgat gctgtcgtac acgggcgagc cagtcttgcg acgctttgcg
    27241 cgcacgagaa gcagtgagcc agtagacgcc caccaccggg taaagcagcg cgctaaacgc
    27301 cgttcttccc agcagacgcc agaccagcag catcaggcgc attccccata atcctttcac
    27361 ttcactctgc cgcgcccagt ggctggaaga gcggcgcatc agcagtgaag gaatacgcgg
    27421 caacatgccg aaaaacagac gcgtgtgcat gagcgagatg cggacgttat ctttcagggc
    27481 atcaaaatgt gaaagcccgt ccagtggata ggtgacgcgg gtcggcacga aatagctggt
    27541 gtttccctgc cagtagaggc gcaccatcac ttcagtgtcg aaatccatcc gcttgccgat
    27601 ggtggcgtgt tttgccagtt gcagcgtcgg cgcaaccgga taaacgcgaa aaccgcacat
    27661 gctgtctttc agttgcaggg agagcgtttc gatccatacc cagacgtggg tgacccagcg
    27721 cccgtaaagc cgtgagcggg ggatggagtc atcgtaaatt ggctggccgg agatcagcgc
    27781 cgcagggtgt tgttcagcga ctgccagcag tttggggata tcttcaatcg catgttgacc
    27841 gtcagcatcc acctgcaccg catggctgaa gcccgcgtct ttcgccgcct gtaagccgta
    27901 catcaccgcc gcgcctttgc ctgcgttttc tgccaggcga attaaagtca cgccaggctg
    27961 ctcggcaagc agactgtcca gttgctgttg cgtggcggca tcgctgccgt cgtccaccac
    28021 aatacagggc agattaaatg gcttaagacg cgccagcacg cccggcatca tcgcgccgtg
    28081 gttgtagcag gggatcaaca cgcagggcga aaagtttacc gacataggcg gatcttcccg
    28141 ctactggcgg tgtggcgggc gtcaccgtcg tgacgctgat agctaaagct cagaatctgg
    28201 cgatcttcct gccagttaag cgtgagcgtg acgcggtttt ccggcagcaa cggagactga
    28261 aattttatgt tctgaatgga gtgaaaacgc cagccaggcg cgagcagggc aagcgcgtaa
    28321 ctcattgccc agtcgatttg tgccacgcca gggagtaatg gttgcacggc aaaatggccg
    28381 cgaaaccaga acagatctgc cctgagatgc aaaacaatct caaggtgatt cgcttgtgcc
    28441 tgatggcgct cgatttcatg gggcttcatg aaataactcc tgtaattgcg catagacacg
    28501 cttgttcata ctgtttactg gcatttcatc aataacgcgc cagtaacgcg gaatggcaac
    28561 aggctccagc gtcgggcgta gtaatcgccg ccaggtcatt tcctggctat gcccgccgcc
    28621 gttctgccat tgcagacgag cttcgtcatc cagcaccagc aaaacgccta tgctctgacg
    28681 cccgccgcgc gtaacaggaa ctgccgccgc ctcgcgaata ccgtccagtg ccagcagacg
    28741 ttgttccact tcctgcaacg aaatacgttt ttcttcaatc ttaacgatgc gtccgcgacg
    28801 ccccatcaga tggaactgac catcttcgct aaactgtaag atatcgtcga gtaacaggcc
    28861 gttatcttcc gtcatcagcg gggaaaagag acgaaatgca ttcccttcgg cccggaactg
    28921 cataccgggg aagggctgcc agcggcgttg ctcctgatcg cggtaacgcc aggcaatgac
    28981 gccggtttcc gtgctgccgt agatttcatc aggccagacg cgcagccagt tcgcggtgtg
    29041 ttgtacgtcc tgccacggta gctcaccgcc agcagagaga agcacctgaa cggggggcgg
    29101 ggaaatctgt gtatccagac gcttcaaaaa tgccgggctg ctgatgaaga tatagcgatg
    29161 ctgatgactc aacgcggcaa actgttcgac ataccagagc atggcggcat gtaggggtaa
    29221 tcccagcgcc attggcagga aaacgcgaaa agtcaggccg tacaaatgct gcggcaatac
    29281 ggaaccgacg acacgacaac ccgccagacg tgcgcctaat cgttcagcca gcagttctgc
    29341 ttcacggtcc agcaaatgaa caggtttaat cacccgcttc ggctgtcccg tggaaccaga
    29401 ggtaaacagc tcgataaaag cctctggcgc tatagcggaa aaggtgaagg attgtgtcgc
    29461 gatttgtgga gaacttgcta ccaacagcaa tgaaccttgc cagttaagtt cgctgtcgct
    29521 aagcacacca tcaaaaagtt cacgttgctc gttaagctgg ataacacggt tatgccccgg
    29581 aagcaccggc gtttttccgg cgtgtagcgt ggcgagcagg gcaacgataa acagatagct
    29641 gttctcaaag cacagcgccc agcgttcacc cggctgttgc tgcaagcggc agataagttg
    29701 cgcgacatcg tgacgcaaat cgcccagcgt ccagaagctt tcgtccagcc acgcaatggg
    29761 tgtgtcatca gggcgtggcg cagtcagcca gcgtgcaagc ggtaaggtct gcttcatgcg
    29821 cgatctcttt tcatcacctg acggcgcagc agccattcac cagccattaa cgtccccatc
    29881 aacaaataag caatcatgcc gttccaggcg gtccataacg acatatcggc ataaagcgcg
    29941 gtaaagaggg caattccacc gttaatgatg aaaaagccgc accagatttg cgtgacgtgg
    30001 cgggtatagc gcacgccctt ttccgggagg tctggctcct gcaaccgcgc cagccgctca
    30061 actatcggca ttgccgacca cagcgaaccg ccaaaaaccg ccagcatgac cgcgttaacc
    30121 actaccgggt aaaacagcag tagctggtgc gttttcaaca taaagctggc gacacataat
    30181 gcgatgccca cgacagcgac gagttgcgtg acggcttgta aaggccccgc ctggcgacgg
    30241 gtctggcgca gacgcaacaa aagcagcaac gccattagcg gtaacagcca gtgcaggccg
    30301 ttatgcgcta agccaaacca aatcacaaac ggccaggcga gcagcaataa tccagtcagc
    30361 agcttaatca tcggtagcga gcgaatgccc gacatcagat caggcttctt gtagcaggcg
    30421 ttctacagcc tcgacgacat cctggactgt acgcacggct ttaaactctt ccggcttgat
    30481 tttcttaccg gttttctttt gcaggtgcac aatcatgtca acggcgtcga tgctgtccag
    30541 ttccagatct tcgtacaggc gcgcttcagg tttgatgtcc tgcggatcga tttcaaacag
    30601 cttaaccagc agggctgaga cttcctgata gacggtttgt tgttctgtca tgatggcttt
    30661 cctcaggcac gttgcgcagc aatgaaagag gccagagtgg cgacggagaa aaagtgctga
    30721 cgcatctctt cgctttccgc agagagcacg atgccgtact cattttttac tgccagtccc
    30781 agttccaggg catcgataga gtccagccct aaaccgtcgc caaacagcgg cgcgtcggta
    30841 tcgatatcat caggcgtcaa ctcgtccaga ttgagggtgc tgatgatgag gtttttaatt
    30901 tccagataaa gcgcttgcat tatttattcc tgagagaggt aaacaacctt gttgtaattg
    30961 aagcaaaaga tgccggttta actgccttgc tgccagcgcc ggttcttgtg aagttgcatc
    31021 gtaaaattta ttgatttcaa cccgaccatg gactgcaacc gtaaaaaacg gccggtcagg
    31081 tggaacgtca taccatttac tttgtttatc cagcagatgc tcgctacagc cgatcgtcac
    31141 gatgcgtaaa tcgctggcac agcgcacggc aatattcgcc gccccgcgct gcagcgtcat
    31201 tttttcaccg ggcttcgttc gcgtgccttc ggggaaaatc aggatagtgt caccctgtgc
    31261 cagtcgctgt tggcagcggg gtaacagggt ttcagcttcg ctgttgatta aataatctgc
    31321 cgcccgcacg acgccgccaa gaaaaggatt ttttaacagc gcgcttttca ccaggcagtc
    31381 agtttctggc atgactgacg ccagcagcac gtaatcgatc agcgtgggat gattggctac
    31441 gacgaggcaa ccgcgttcct gacgtaaaat ttcagcgcca tcaatacgat aatccagcac
    31501 gccaagccct ttcgcgacag ttaaaaacag gcgaaaactg gcggcaatgc tgcgacgcgc
    31561 caaacgacgg cggcgggaag tatcccacac caaaactaac aatatgttaa accacacgac
    31621 agaaagcagc aggccgccga gaccaaacag ggcaaaacat aacccggtca tgaccagtcg
    31681 ccaggtccac tccagacgtg acatcagctt actcatctgt ggctccagcg ccattgcacg
    31741 cgctcgccag gcagtgaaaa tgtgtctaca ttttgtaaat agtgctgtaa aaacaacatg
    31801 ctttgcggca gtgttgtttc attgcccgca atggctgact gcgtttcaca ttgccagtca
    31861 tcgccagctt caatcaccag cgcgacggcg tacggccagg tcggcatttc tgatggcagt
    31921 tgtgggtgat aaaactcggg caggaaaccg tcaaaatcga ccatcagcac gcgctggtat
    31981 cccgcttgta acaggctaag cacttcacac aaaccttgct ggaaagtgtc gcgaccagca
    32041 gaaagcgatg acgaaacgat cggctgtttg gccgcaatgg tcaggttacc cacggaagag
    32101 ttgtgtactg aaagtgcaaa gtcggtcgga gaaagcgcct gttcggttgc cagtgcatga
    32161 acaatgcggt aattgcgctc cagttcgcca tgacgactgg tatacaaaac ggcgtctggc
    32221 tgatggcggc gtagcattgc cagaccgcat tcaacagcca gtttgctgcc tgaactgagg
    32281 cggcgggcgg tcatcattgg cagttcactt aattttgcca acggcgcggc agggtcaatc
    32341 gcccacggct ggcgcgacca tgcttgccat tgctgcgctt cgctaagtcc aggcgccagt
    32401 gcctgccagt cggtaatatt taatgtaaat ttcatcaacg cgcgcccgcg gtaaaccaaa
    32461 attattaagt gacgattatt tcttttggca tatcaaaagt gtatgcccaa cgcctaaatt
    32521 atcgtggcgc tctgctacct ggaatcctgc tttattcaga tagtcgtaaa atttctctgc
    32581 actatagaaa cggctgttac cgtttgccat gcaggtaaaa taaagtgagg acgcgttcag
    32641 gctaaatgat gctgcttcaa accgctgcgc atcccagaac aactccatga tgcaaacttt
    32701 tgcgccaggc ttcattacgc tggcgatttt gctgagtatg ctaattattt gttcgggtga
    32761 aaaacagtct aaaaattggc tcatccacca gatatcagcc tcgcccggta atggggcatc
    32821 gcttagcata tccaccgcat ggaaatctat gcgatcagaa aaacctgcat tcgcgatatt
    32881 ttctttcgct agtacgattt gttgtggcag atctaataac gtcacggcga tgttttcatt
    32941 gtatttgcag caacgcaaag cccacttacc cgtattgccg cctacatcgt acaatgttgt
    33001 ggggttgttg gcgaatacat aaggcagtgc ggcgttaaat gcgccatctg aatagtaatg
    33061 atcaaacgca aaccagcttt cacgagcggc gtctggtaat tgcgaaagtg ccggatatat
    33121 cgtcgaccag tcgccaaata cttttaaccc ggaaggtttt ccttcattga gtgaatcggc
    33181 gaggaagaag agcccctggt aacagacatc ctgggtgaaa tccatattca cgcgagtcat
    33241 agtgtcatgc aggaggtaat gaccgatttt cgccaggtaa taaattccct ctttacaggt
    33301 aataattcgc ccgcttaagc ccatatctaa cagaacgctg gcagcatatt cgttgatatg
    33361 gctatgttcg gtgatatcag ccagcgttgc gccatgtttg ccttgccgat ccagatatga
    33421 aagtacaccc gcattacgta ggcagagtgc tgtttgaaaa agcatagggg caaaggcaat
    33481 gcgttgagct tcggtaatcg catccagtgc gctaagagaa tcctgttcgt acatgttggg
    33541 cacaacctgt taagagggaa ttaaattatt atcggaccgc aacggcccga ctttaggaag
    33601 aattataaat ctgcgcccgc tgacaaattc agttgaatat ctttgtcgag attgcggacc
    33661 cagcgattgt agtttttatg aatttgtccg cctgacgctt gtaggttctg gctattttca
    33721 taattgatat cgtagctgga tgccgtgtag ttaatgcgta tttccgctga atgatcgcgt
    33781 gactgttgac gtgctttaat tatgccttgt ccggcttcgg acataatcca tttgcgttca
    33841 acgccagctt tcagaatcgc ttttttcact tgttcctggg tatgaccagc gctgacggtt
    33901 gagtgaacct gaacaattgg cgcagtacgc gcgcaacccg ctaaagcacc cacaacagca
    33961 atagccgcaa accatttcat gtaatgtctc attagaaccc cattaaagta ttgtttttac
    34021 gagtaaagca agttttaact gtaattgctg gcatcactat tctttattta gagaaaaaat
    34081 tacttgtgag ataagtcagt aagtgcacat cagagtaaaa atctctgaaa tgactaaatt
    34141 actgccagca gggaccaact ataagtgagt gagaaaaatg aggcaataaa aataacacaa
    34201 tgttatcagt gcattctttg agaaggattt aa
//
LOCUS       DAZUGK010000028        33991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZUGK010000028 DAZUGK010000000
VERSION     DAZUGK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.028"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(132..1682)
                     /gene="lsrK"
                     /locus_tag="ACHY5W_001206"
     CDS             complement(132..1682)
                     /gene="lsrK"
                     /locus_tag="ACHY5W_001206"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFS4446137.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDP
                     EMNVRINPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMA
                     SRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSA
                     CNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAA
                     GVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTT
                     SLWKAPGL"
     gene            complement(1761..2756)
                     /gene="lsrR"
                     /locus_tag="ACHY5W_001207"
     CDS             complement(1761..2756)
                     /gene="lsrR"
                     /locus_tag="ACHY5W_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFS4446138.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRSEICMTLLPGMMENV"
     gene            3005..4540
                     /gene="lsrA"
                     /locus_tag="ACHY5W_001208"
     CDS             3005..4540
                     /gene="lsrA"
                     /locus_tag="ACHY5W_001208"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFS4446139.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPRHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4534..5562
                     /gene="lsrC"
                     /locus_tag="ACHY5W_001209"
     CDS             4534..5562
                     /gene="lsrC"
                     /locus_tag="ACHY5W_001209"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFS4446140.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSMQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKRETA"
     gene            5562..6554
                     /gene="lsrD"
                     /locus_tag="ACHY5W_001210"
     CDS             5562..6554
                     /gene="lsrD"
                     /locus_tag="ACHY5W_001210"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFS4446141.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSDVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANTPLP"
     gene            6566..7588
                     /gene="lsrB"
                     /locus_tag="ACHY5W_001211"
     CDS             6566..7588
                     /gene="lsrB"
                     /locus_tag="ACHY5W_001211"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFS4446142.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7615..8490
                     /gene="lsrF"
                     /locus_tag="ACHY5W_001212"
     CDS             7615..8490
                     /gene="lsrF"
                     /locus_tag="ACHY5W_001212"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFS4446143.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTSKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNR
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8514..8804
                     /gene="lsrG"
                     /locus_tag="ACHY5W_001213"
     CDS             8514..8804
                     /gene="lsrG"
                     /locus_tag="ACHY5W_001213"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFS4446144.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8861..9619
                     /gene="tam"
                     /locus_tag="ACHY5W_001214"
     CDS             8861..9619
                     /gene="tam"
                     /locus_tag="ACHY5W_001214"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFS4446145.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMVAEARSALPDCQFVEADIRNWQPEQTLDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCKVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9623..10537)
                     /locus_tag="ACHY5W_001215"
     CDS             complement(9623..10537)
                     /locus_tag="ACHY5W_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFS4446146.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10734..12185)
                     /gene="uxaB"
                     /locus_tag="ACHY5W_001216"
     CDS             complement(10734..12185)
                     /gene="uxaB"
                     /locus_tag="ACHY5W_001216"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4446147.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISIYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12412..13830)
                     /locus_tag="ACHY5W_001217"
     CDS             complement(12412..13830)
                     /locus_tag="ACHY5W_001217"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4446148.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVSSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13969..14328)
                     /locus_tag="ACHY5W_001218"
     CDS             complement(13969..14328)
                     /locus_tag="ACHY5W_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFS4446149.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRFGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEQQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14328..15254)
                     /gene="glsB"
                     /locus_tag="ACHY5W_001219"
     CDS             complement(14328..15254)
                     /gene="glsB"
                     /locus_tag="ACHY5W_001219"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFS4446150.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15318..16706)
                     /gene="sad"
                     /locus_tag="ACHY5W_001220"
     CDS             complement(15318..16706)
                     /gene="sad"
                     /locus_tag="ACHY5W_001220"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFS4446151.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16807..17688
                     /locus_tag="ACHY5W_001221"
     CDS             16807..17688
                     /locus_tag="ACHY5W_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4446152.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSARQGYQ"
     gene            17766..18881
                     /gene="yneK"
                     /locus_tag="ACHY5W_001222"
     CDS             17766..18881
                     /gene="yneK"
                     /locus_tag="ACHY5W_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFS4446153.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     PPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDIPDRH"
     gene            19032..20222
                     /gene="ydeA"
                     /locus_tag="ACHY5W_001223"
     CDS             19032..20222
                     /gene="ydeA"
                     /locus_tag="ACHY5W_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFS4446154.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20247..20912)
                     /gene="marC"
                     /locus_tag="ACHY5W_001224"
     CDS             complement(20247..20912)
                     /gene="marC"
                     /locus_tag="ACHY5W_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFS4446155.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21124..21558
                     /gene="marR"
                     /locus_tag="ACHY5W_001225"
     CDS             21124..21558
                     /gene="marR"
                     /locus_tag="ACHY5W_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFS4446156.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21579..21962
                     /gene="marA"
                     /locus_tag="ACHY5W_001226"
     CDS             21579..21962
                     /gene="marA"
                     /locus_tag="ACHY5W_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFS4446157.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21994..22212
                     /gene="marB"
                     /locus_tag="ACHY5W_001227"
     CDS             21994..22212
                     /gene="marB"
                     /locus_tag="ACHY5W_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFS4446158.1"
                     /translation="MKPLLSAIAAALILFSAQGFAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22243..23142)
                     /gene="eamA"
                     /locus_tag="ACHY5W_001228"
     CDS             complement(22243..23142)
                     /gene="eamA"
                     /locus_tag="ACHY5W_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFS4446159.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVVVLIEDSLNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23337..24524
                     /gene="ydeE"
                     /locus_tag="ACHY5W_001229"
     CDS             23337..24524
                     /gene="ydeE"
                     /locus_tag="ACHY5W_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFS4446160.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSIDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            24651..24746
                     /gene="mgtS"
                     /locus_tag="ACHY5W_001230"
     CDS             24651..24746
                     /gene="mgtS"
                     /locus_tag="ACHY5W_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFS4446161.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            24746..24850
                     /gene="mgtT"
                     /locus_tag="ACHY5W_001231"
     CDS             24746..24850
                     /gene="mgtT"
                     /locus_tag="ACHY5W_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFS4446162.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(24965..25855)
                     /gene="dgcZ"
                     /locus_tag="ACHY5W_001232"
     CDS             complement(24965..25855)
                     /gene="dgcZ"
                     /locus_tag="ACHY5W_001232"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFS4446163.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KLTGRNRCMFIDEQNVINRV"
     gene            complement(26110..26501)
                     /gene="ydeI"
                     /locus_tag="ACHY5W_001233"
                     /pseudo
     CDS             complement(26110..26501)
                     /gene="ydeI"
                     /locus_tag="ACHY5W_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
     gene            26777..27295
                     /locus_tag="ACHY5W_001234"
     CDS             26777..27295
                     /locus_tag="ACHY5W_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFS4446164.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(27340..29385)
                     /gene="dcp"
                     /locus_tag="ACHY5W_001235"
     CDS             complement(27340..29385)
                     /gene="dcp"
                     /locus_tag="ACHY5W_001235"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFS4446165.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRFATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            29522..30268
                     /gene="ydfG"
                     /locus_tag="ACHY5W_001236"
     CDS             29522..30268
                     /gene="ydfG"
                     /locus_tag="ACHY5W_001236"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFS4446166.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            30357..31043
                     /gene="rspR"
                     /locus_tag="ACHY5W_001237"
                     /gene_synonym="ydfH"
     CDS             30357..31043
                     /gene="rspR"
                     /locus_tag="ACHY5W_001237"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFS4446167.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            31220..31423
                     /gene="ydfZ"
                     /locus_tag="ACHY5W_001238"
     CDS             31220..31423
                     /gene="ydfZ"
                     /locus_tag="ACHY5W_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFS4446168.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(31459..32919)
                     /locus_tag="ACHY5W_001239"
     CDS             complement(31459..32919)
                     /locus_tag="ACHY5W_001239"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4446169.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(33009..>33991)
                     /gene="ydfJ"
                     /locus_tag="ACHY5W_001240"
     CDS             complement(33009..>33991)
                     /gene="ydfJ"
                     /locus_tag="ACHY5W_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFS4446170.1"
                     /translation="LGAGADISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAI
                     WAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKP
                     APAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSIL
                     GFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHN
                     CAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYP
                     IAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
BASE COUNT         8749 a         8571 c         8113 g         8558 t
ORIGIN      
        1 tatattactg gtgatggaag gatgaatctg caagctggca tttaacaagc cagttctaaa
       61 agaagggaaa taagaataac tatactcaaa aactaacagc cacggtcatc atgatgtggc
      121 tgtcaatgaa actataaccc aggcgctttc cataacgacg tcgtcagtcc atgatcaacc
      181 agccccagct gatcctgata aactgcctgc cacttatcgc gtgaatcctg ataaagttca
      241 tgcttttccg ggtctggtgt gtgcgtccgt tcccagcgaa ccaggcgttc tccggtttct
      301 gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa tggcacatcc taatgcggtg
      361 gcttctttga ccaccggaat attgacgggt aatcccgaga catcagcgag aatttgactc
      421 cataatttcc cttttgaacc tccgcctgca aagactaacg atgaaggatg aatattcgaa
      481 aaatcagcaa tttgctgcaa gttacacgct gatacaatcg ccgcattttc ttccagcgca
      541 cggaacaatg tcgctttgtt acatttatcc gggtcaatgg acaaattaat aaaggaaggc
      601 gcagcgtgat accaggtttt aaagcgcatt ctgtcggaga agatcggcat tacgccccac
      661 gacccaggcg gcacccgact ggccatctct tccagcagcg tataggtgtc gatgcctaaa
      721 cgttcggcaa tcagtttttc ttcggcacag aaagcatcgc ggaaccagcg catggtgagt
      781 ccggtaaaaa agcttataga ttcagcttgt accatgccag gaataacatg aggattaatg
      841 cgcacgttca tttctgggtc tgtcaccggc gcggctaaat ttacaacttg ctgccagaat
      901 gtgccgccaa gaaccgcggt ttgtgccgga cgcacaacgc ctaaccccag gcaaccaagc
      961 tgcacgtcgc ctcctccaac gaccaccgga gtgcccacct tcagaccgca gagttccgcc
     1021 gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt tgacaggaga aagaatatcg
     1081 gcacgcaggc cagccatatc cagcaatgca ggtttccagt cacggatggt tagatcaaga
     1141 agtcccgtgg tgccagcgtt agagggatcc accgcaagtt cgccgctgag catataggcc
     1201 agccagtcgc tgatcatggt gatggttgat gcctgacggt aaatatcgga acgatggtgc
     1261 gccagccaaa gtaatctggg gatggcactt aaagccagtg tttgtccggt cgcgcgataa
     1321 acttcgtttt caaaggtatt gttgtgcagt tctttaagtt cgctaacttc acgcgccgct
     1381 ctggcatcca cattggcgca ggcccagatc ggtgttcctt cattgttata taaaacaatg
     1441 ccttcacgca tcgaacatgc cgaaacggca gcgatatact ccggggctat gccggcgttg
     1501 tgcagcgcct ggcgcataca ctcacacgcc agttgccagt ttttgttgag atcaaattcc
     1561 atagaaccag gaacgtccgg tactgccaga tgccgccact ccgcctgtcc cactgctatt
     1621 tgattgcctt ccaggtcgaa tatcacagcc cgaatacttc cggtgcctgc atccagcgcc
     1681 atcaggtaat actttgattc ttaaggggta aagagtcgag ccattagatt atcctcggct
     1741 tatgcgttct gtctgttcct ctatacgttc tccatcattc ccggtaataa ggtcatgcaa
     1801 atttcactac gtaaaatcgc cgctgctgtg tcctgatcgg taaccagtgc gttgatataa
     1861 ccgcctttca ttgcagcggc aattgcttcg gctttatttt ctccccctgc cacgccaacc
     1921 cggacgggta tggtcttcag cgcgcttaaa ggtaagccaa tcagttcgtt atgtatttta
     1981 atatccgtga caacgtcacc ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc
     2041 ccttttcggc caatcattaa ctgttcgccc tggctgatat aaccggagcg aatgatcgtc
     2101 gcgtcgtctt gttgactcac ggccccaatg ccgacaatcg ccacatccgc tgcttgcgcg
     2161 gctaacagaa catctttgac gcaattttca ttttttagcg tacgggcaat gtcagcggag
     2221 gatgcccgca acggagccgg aataatattc acactgcacg ccgcgttaag ctgcccgata
     2281 cccgtcatat aagaaccgac gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa
     2341 ataaaaccac ttaagcgttg cagcgtattc atagttgcct cgccaaaacc aatcgccagc
     2401 atctgttgtg gttgaagtaa actcatcaac atatgcgccg cgcctatccc cagtcgcccg
     2461 ccgacatcag catcggcaag cccagggatc acccggacat gttgcagcga aaactgacga
     2521 cgtaattgtg tttcatattc cagacagcct tcaaagcgag aattaatctg cacgcgaatg
     2581 atgccggact gatgcccttt ctccagtaat cgcgacactt tcaaacgggt caggccgaga
     2641 cgatcgctga tctcactctg ggtcagcccg tcgtggtagt aaaaccacgc gatccgcgcg
     2701 acctgttctt cttcacacat tccctgttct gaaattaccg aatcgttgat tgtcataatt
     2761 cattcttcac tttgaacata tttaaatctt taatgcaatt gttcagttct tgcgcattta
     2821 tatctgtgat ggcaaccaca gtttgactct acgagcatga acaaacgcaa ccgtgaaaat
     2881 caaaatagca taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta
     2941 tgaacaaatt aaaagcagaa atacatttgt tcaaaactca cctgcaaaac tgaacggggg
     3001 aaatatgcaa acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa
     3061 acagtattca ggggttaatg tcctgaaagg catcgatttt acgttgcatc agggggaggt
     3121 ccacgccctg ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg
     3181 tattacccct gctgatagcg gtacgctgga gattgggggc aacaactacg ccagattaac
     3241 gccagttcat gctcatcagc tgggtattta tctcgttccc caggaaccgc tgcttttccc
     3301 aagcctgtcg ataaaagaaa acatcctgtt tgggctggca aaaaaacagc tctccatgca
     3361 aaaaatgaag aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg
     3421 atcgctggat gtcgccgatc gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc
     3481 gcggattctg atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt
     3541 gtttagtcgc ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa
     3601 gctgccggaa attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc
     3661 cttaagcggc aaaaccagcg aactgtctac cgacgacatt attcaggcca tcacgccagt
     3721 ggtacgggaa aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggtaaccg
     3781 cccacgacat gccgcaggaa cgtcggtgct gacactggaa aatctgactg gcgaaggttt
     3841 taggaatgtc agcctgacgc taaatgccgg agaaattctg ggtctggctg ggctggtggg
     3901 tgccggacgc acagaactgg ccgagacgct ctatggtcta cgtactttgc gtggcggacg
     3961 cattatgctg aatgataaag agatcaatag attctccact ggagaacgtt tactgcgcgg
     4021 tctcgtttat ctgccggaag atcgccagtc ctccggactg aatctcgatg cttcgctggc
     4081 ctggaacgtc tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga
     4141 taatgccacc ctggaacgat atcgtcgggc gctgaatatt aaatttaacc aaccggaaca
     4201 agctgcacgg acattatccg gtggcaacca gcaaaaaatc ctcattgcca aatgcctgga
     4261 agcctcgccg caagtattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcgcg
     4321 taatgatatc taccaactgt tgcgcagcat cgccgcacag aatgtggctg tgctgcttat
     4381 ctcctctgat ctggaagaga tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg
     4441 cgaaattacc cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt
     4501 tgccttcggc gatagtcagc gtcaggaggc ggcatgctga agtttattca gaacaaccgt
     4561 gaaatcacgg cactgctggc ggtgctgctg ctgtttgtat tacccggttt tctcgaccgc
     4621 cagtatttaa gtatgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg
     4681 ctggcaatgg gcgcgacgct ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg
     4741 attaccggaa tgtgcgcggt gctgttgggg atgttactga acgcggggta ttcactacct
     4801 gttgcctgtg tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg
     4861 gtcgcgtggc taaagatccc tgccattgtt gccacccttg gcacgttagg gttgtacaga
     4921 ggcatcatgt tgctgtggac tggcggtaaa tggattgagg gattacccgc cgaactgaaa
     4981 caactctccg ccccgctact gtttggcgtt tccgcgattg gttggttaac aataattctg
     5041 gtggcattta tggcctggct gctggcgaag acagcgtttg gacgcagttt ttatgccacg
     5101 ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg
     5161 gcattttcgt tgaacggctg catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt
     5221 ggttttatcc ccaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg
     5281 ctgggcggca ttagtttgct cggcggttcc ggtgcgatca tcggtgcggt actcggcgca
     5341 tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc atggtggaac
     5401 gattttatcg cgggcctcgt gctgctggcg gtgctggtgt ttgatggacg cctgcgttgt
     5461 gcgctggaac gtaatctacg gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc
     5521 gttaaacccg cttcgtcagg taaaaaacgg gagaccgcat aatgcgtatt cgctacggtt
     5581 gggaactggc tcttgccgca ctgctcgtta ttgagattgt cgcatttggt gcaattaacc
     5641 cgcggatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca
     5701 ttgtcgccct accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt
     5761 cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtgat gtgccgatgc
     5821 cgctggcgat tctcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat
     5881 taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg
     5941 ctggaagcgc tctgctgctt tccgggatgg ccggagcgac ggggtacgaa ggtattggtg
     6001 gattcccgat ggcgtttaca gatttcgcta acctggatgt gctgggactc cccgttccgc
     6061 tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg
     6121 gacgtaatgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc gcgattccag
     6181 ttaaccgcac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg
     6241 tgctgctggt atcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc
     6301 ccgccatcac cgccgtggtg cttggcgggg ccaatattta tggtggttcc ggttccatta
     6361 tcggcaccgc cattgcggtt ttattagtgg gatatttgca acaaggtttg caaatggcag
     6421 gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag
     6481 gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata
     6541 ccccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact
     6601 tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt
     6661 tattcccaaa ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc
     6721 gggtaaagag ctgggcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg
     6781 tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta tcgtttctgc
     6841 ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt
     6901 gctgacctgg gactctgata ctaaaccgga gtgccgctct tactacatta atcagggaac
     6961 gcccgcccag ttgggaggta tgttggtgga tatggcggcg cgtcaggtga ataaagacaa
     7021 agccaaagtc gcgtttttct actcaagccc caccgttacg gaccaaaacc agtgggtgaa
     7081 agaagcgaaa gcgaaaatcg ccaaagagca tccaggctgg gaaattgtca ctacgcagtt
     7141 tggctataac gatgccacta aatcgttaca aaccgcagaa ggaatattaa aagcgtatag
     7201 cgatctcgac gccattatcg cccccgatgc caacgccctg cccgctgccg cacaagccgc
     7261 agaaaacttg aaaaatgaca aagtagcgat tgtcggattc agtacgccaa atgtgatgcg
     7321 cccgtatgta gagcgcggca cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg
     7381 caaaatttca gtgtatgtcg cggatgcatt attgaaaaaa ggatcaatga aaacgggcga
     7441 caagctggat atccagggcg taggtcaggt ggaagtctca ccaaacagcg ttcagggcta
     7501 tgactacgaa gcggatggta atggcatcgt actgttaccg gagcgcgtga tattcaacaa
     7561 agagaatatc ggcaaatacg atttctgatg tgcattactt aaccggagta agttatggca
     7621 gatttagatg atattaaaga tggtaaagat tttcgtaccg atcaaccgca acaaaatatc
     7681 ccttttaccc tgaaaggttg cggtgcactg gattggggaa tgcaatcacg cttatcgcgg
     7741 atatttaatc cgaaaacgag taaaaccgtg atgctggcct ttgaccatgg ttattttcag
     7801 ggaccgacta ccggacttga acgcattgat ataaatatcg ccccgctgtt tgaacatgcc
     7861 gatgtattaa tgtgtacacg cggcattttg cgcagcgtag ttccccctgc gaccaatagg
     7921 ccggtggtac tgcgggcgtc aggtgcgaac tctatcctgg cggaattaag caatgaagcc
     7981 gtggcgttat cgatggatga cgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt
     8041 tatatcggca gcgaatatga acatcagtcg atcaaaaata ttattcagct ggttgatgcc
     8101 ggaatgaaag tgggaatgcc gaccatggcc gtgactggcg tgggcaaaga tatggtgcgc
     8161 gatcagcgtt atttctcgct cgcgactcga atcgccgctg aaatgggggc gcaaattatc
     8221 aaaacctatt atgtcgaaaa aggttttgaa cggattgttg ccggatgtcc ggtacccatt
     8281 gttattgctg gcggtaagaa attaccggag cgcgaggcgc tggaaatgtg ctggcaggct
     8341 atcgatcagg gcgcttctgg tgtggatatg gggcgtaata ttttccagtc tgaccatccg
     8401 gtggcgatga tgaaagccgt acaggcggtg gtacaccata acgaaacggc tgatcgggca
     8461 tatgaactct atctgagtga aaaacagtaa ctgcggattt aaggagaaga attatgcacg
     8521 tcacactggt tgaaattaac gttcatgaag acaaggttga cgagtttatc gaagtttttc
     8581 gccagaacca cctgggctct gtacaggaag aaggcaattt gcgcttcgat gtcttacagg
     8641 acccggaagt gaattcgcgc ttttatatct acgaagccta taaagatgaa gacgcagtgg
     8701 cgttccataa aaccacgccc cactacaaaa cctgtgtcgc gaaactggaa tctttaatga
     8761 ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc gtgaggcgaa tttatcaatt
     8821 ttatctacaa ttggggtaac gcgctgacgg gagtaaaaaa atgtctgact ggaacccctc
     8881 tttatatctg cactttgccg ctgaacgatc gcgtccggcg gtggagctgc ttgccagagt
     8941 gccgctggag aatgtcgaat atgttgccga tcttggctgt ggcccaggta acagcaccgc
     9001 ccttctacaa caacgttggc ctgctgccag gataacgggc atcgactcat ctccggcgat
     9061 ggttgccgaa gcacgcagtg ctttgccgga ctgccagttt gtggaagcag atatccgcaa
     9121 ttggcaaccg gaacagacac tcgatctgat ttttgctaat gcctcactgc aatggctgcc
     9181 cgaccactac gaattgtttc ctcatctggt ttcgttactt aatccgcagg gcgtgctggc
     9241 agtacagatg ccagataact ggctggagcc gacccatgtg ctcatgcgcg aagttgcctg
     9301 ggaacaaaac tacccggatc gcgggcgtga gccgctggca ggcgttcatg cttactacga
     9361 tattttgagc gaagccggat gtaaggtcga tatctggcga accacttact atcatcagat
     9421 gccgtcgcac caggcgatta tcgattgggt gaccgccact ggattacgtc cgtggttaca
     9481 ggatctgacc gagagcgaac agcagcattt tcttacgcgc taccatcaga tgttggaaga
     9541 gcagtatcca ctgcaagaga acggacagat actgctggca tttccgcgtc tgtttattgt
     9601 tgcccggcgt acggagtaag tatcacgtca gctggtaatg acgatcggga agaatttttg
     9661 ctggaatttc ggcttcatcg ttcatctgta agaggtcaat ttcaatagcg ttgcagatgg
     9721 catccagtgg taaatcattg ttttcagtac cgaatggatc ttccagttct tccgccagac
     9781 aatccagcga aataaaagtg taggaaatca gcacagagat aaaaggcgtc atgtaatgca
     9841 ggtccacgac cagcgcgaac ggcagcatga tacaaaacag ataaacggta cgatgcaaaa
     9901 tcagggtata ggcaaaggga attggcgtgt aggcaatgcg ctcgcagcct gccagaaccg
     9961 ctgaaatatc gttaagccga tcgttgaggc taataaacag gatatctgaa agttgtccat
    10021 tgcgtcgctg aaccgccaac cactccccca ttattaacaa gatgcggtta gctggagagt
    10081 tcgaagccag tacacgctga agatcttcag tcttgagata atgagccagc acttccgcct
    10141 gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc agccgggcaa
    10201 actcccttac acttgccgaa tccggcaatg tcgtttttac ctcgcgcagt aacgaccgtg
    10261 aggcaatcat caactgcccc caaagttttc gcgcttcaac gtaacgggcg taaccggcat
    10321 tattacgaaa accaagaaaa atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg
    10381 tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa aataacagcg atagaaaaga
    10441 gaaaattgag tagtaagcgc gaggatatct tggataatac tgagccatgc cagacaaaaa
    10501 tacggcgcag ccagtgttgt tgtggacgaa cgatcatggt tatcttcagg cgtggaaaag
    10561 tcgccctatt aaacgtgatt acgatcacat tctcaagaca cgacttacaa attacctaca
    10621 agcttacaac attaacaggt gctgcatgtg gcacctgtta atgttgtaag cttgtaggat
    10681 gttgtaacta atatagtgaa ataagaaacc cggtcgaaac cgggttcaga gtattagcac
    10741 aacgggcgta cagcttcgcg catccctttt tcgagaatcg catccaggtc attagccacc
    10801 tgctcgacca aaccaggcac ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac
    10861 acaatcgcaa ccagttcctg ggtaccgata acacggtcac gatgctggct ccagagttgc
    10921 tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc gttacgctca
    10981 ccgcgataga acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg
    11041 tttgccttct gccctgccag tagctgcggc aggatgcggg tgcggaactt ggtcataccg
    11101 ttgagcgcga tagacagcag ctgatgcttg atgtacgggt tacggaaacg cccggtgact
    11161 gcactggcga aagactccag ttcatcgcga ggcaaatcca gaaccggaat aatttcttcg
    11221 tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta
    11281 tccagccctg cctggaacgc caccggcacc agcgcggtat gtgcaccgtt gaggattgcc
    11341 actttgcgtt ctttatacgg tttaatatcg tcaacaatca gcacgttgag cggatatttg
    11401 tccagacgca gctcggtcgc taaggatttc ggtccctgaa tcacaaacag gtaaaagtgt
    11461 tcagcggtgt cgaggaaacc atcgtgataa cccagttctt cttccagttt agctacttca
    11521 tcacgcggat aaccggtaac gatacggtcc accagcgtag aacagaagct gttagcctga
    11581 tccagccatt gaataaatgc ttctggcagt gcccactctt gcgcatagcg cagcaccagt
    11641 tcacgcaacg cgtcgccatt gtagtcgatc aactcacacg ggatgatgat ccaaccttta
    11701 tccagcgcac cgttgaaatg gctaaaacgc tcgaatagca gacgggtcag ttttgcggga
    11761 taacttaccg ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat acccgcttcg
    11821 gtggtgttag agaaaacaaa gcgcatttcc gggttgtgcg ccagtttcag gaattcatcg
    11881 tattcactgt agatgctgat ttcacgatta accgagcgaa tcagacgcgc gtcgctgacc
    11941 gcttccccct tctcattcag gccacggata atggtggtgt acagaccatc ctgcgtgctc
    12001 agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga ccacgccaga attcagatcg
    12061 gtgtgctcat tcaggagatc gatttgccag tcaacaaagg cgcgcaggaa gttaccttca
    12121 ccaaactgaa tgatacgttc tggatactgt gcaccgggaa aatcgcgacg atttagtgtt
    12181 ttcacaatgg gttcccttct gattagtcat acaacctgtt tgaattggta cgacaggtta
    12241 gcaaacttta atacgacgcc cccctgtttt gatcaactcc tgatgattaa tgagcagttt
    12301 tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga aaaacgcctt ttcctggatc
    12361 ataaagtggt agaacacatt gcatttaaat cgctctgaat gaacaaagat gtcagacaac
    12421 ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg agcgatacag
    12481 acacgtatcg gcttcgacca tcaatttatt aaagtcatcg gtcagcgttc ggtacgatgc
    12541 gcgaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt gccaggtgaa
    12601 tggttgcagt tcaacgcctt tacggatttt ttccgccatt agcagaccat cgacaggatt
    12661 caccgacggc actgcaacag caaattcttc gccgcccatg cgcgccacca accccttatc
    12721 accgacaatc ttctgaatat gccgggcaaa cacgcttaac actttatcgc cacattcatg
    12781 tccatagtta tcgttaatac ttttgaaata gtcgatatca agcagcatga cagtcagatg
    12841 ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc gtgagtaaac
    12901 ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg aattgatcgc
    12961 tgccacgcta aaagaaacca taattgggca tatcgccatc gtggcaatac cgagacgtgc
    13021 ggagaacatt tccggaatgg agaacggcga actgaccgag atatcaatca ccgaatttgc
    13081 caccagtacg atttccaccg caccggtaac aaaggtcaac agacatgtta cctgcggcgt
    13141 atagcgcact gcacaccaga ttaatgcagg cagcgggaac gccagactcc ccgctccgcc
    13201 aatgactacc gaggcaataa ccgacacaat aagcgcgatg gcgggcatca tctgttctgc
    13261 tttaaagcgc ggcagcactc caggaatagc caacgtcagc atacaaggca cgatcaacac
    13321 gcccgttgag aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc
    13381 aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta
    13441 attaaatagc cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg
    13501 cacgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga tagccagtga
    13561 aaccagtccc cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc
    13621 atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca ttacgccatt
    13681 cagcggccag aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa
    13741 cagcgtggtt agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacac
    13801 acggaaagtt gaaatggggt gtacatgcat taattatctg tctatacaat tgcggatggc
    13861 aattatctca acgtaataag taaattctca taaaggatag catgttcgct gacagcggcg
    13921 gcaaataaag gtaccctggg aatgctattt tatcgttttc cgcaattatc atggctgatt
    13981 catctgtata accagccagt gatagattac cgccacgata taacgttgtt gttgctcgct
    14041 taacgaaaca ccctgcggaa tgttatgcca cttagccaga caaccgcgac aacaggttgc
    14101 agtggcgtgc tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt
    14161 agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac
    14221 tggcgcgcct ttctccagac aatactgacg ctctttcatg ccaaaacgaa agcgagatcg
    14281 aaattttgaa cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa
    14341 cgccctaact gtttcgtcaa ttgttcaagg acggcaatgc ccgcaagcga gttacctgca
    14401 tcatccagtt ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata
    14461 ccgccaccaa cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc
    14521 tggtacatac cactggtcgc catcagcgcg ttaatttgcc gtgcctgcat tggcgtcacc
    14581 acaggttcat caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgggcc
    14641 agctctacac agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc
    14701 acgtcatgat ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg
    14761 gaatgttcaa attcggaacg cgctaccacc gtatcgtagg aaatatcaga cacaccgctt
    14821 aagccgcgca cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata
    14881 tcgcagacca ccagcgcacc ggcattaata aacggattac gcggaatacc ctgctccatt
    14941 tccagttgca ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag
    15001 atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac
    15061 tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag
    15121 atagcaatgc ccaatcggga accgtctacc gtagccagcg ccggaatata atccgcgact
    15181 ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc
    15241 atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca
    15301 gagctgaata tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg
    15361 tgtaagccga aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc
    15421 actcgcgcat cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc
    15481 gccatctgtc tggcctgtgt ttcgtcagtg gtaaaaatgg tcgctgaaag gccgaactca
    15541 ctatcattag ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg
    15601 gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt
    15661 ggcggatagt agttacctgc cccagccatc ttttccccgc ccagtagcaa acgcgcaccc
    15721 tgcgccaggg ttttctccac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt
    15781 ggtccgagag tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc
    15841 acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga tgataaaacg ttttgccgct
    15901 gcacaaacct gtccggtatt ctgataacgt ccggctaccg ccgctttcac cgccagttcc
    15961 agatcggcat cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt
    16021 ttcagtgccg ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc
    16081 gtgatagcag caatgcgcga gtctttaatc atctgagtga caccgtcatt gtcggcattc
    16141 agccagccat atacgccttg tgggattccc gcatctttaa acacctgggc aatgagctga
    16201 gcacagccca tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg
    16261 ggaacagcgc cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc
    16321 gtccccaacg gtcgatactc aataactgcc tgctgatttt ccaccagcgt aggttccgcc
    16381 ttcagcattg ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact
    16441 tcagcgcgcg cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct
    16501 tcgctacggg cgcgcagagc cttaccgata tcacgcagtt tttcagcacg ataatctata
    16561 tttgtctcgc gccagtcacg aaagcctgct gccgccagct gaagtgcgtt ttcgatatca
    16621 tcagcgccag cccacggcag cacagaaagt tgttcacccg tggcaggatt tatcgaaatt
    16681 gcatgagttg ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga
    16741 tacgcagatt tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag
    16801 aatattatgg atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt
    16861 acgcaggctg cagcaatagt gcatcgcgtg ccgtcgaatc tcactacccg tctacgccag
    16921 ctggaaacag aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctatct
    16981 cctgccgggc ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg
    17041 cggagcgttg tcgctggcga tgagccgcaa ggtttatttt ctctgggttc gctggaaagc
    17101 actgcagcag ttcgcattcc ggccacgctg gcggaattca accgtcgtta tcccaaaatt
    17161 cagttttcac tttccaccgg cccttccggc actatgctgg aaggtgtgct ggagggaaaa
    17221 cttaatgcgg cgtttattga tggacccatt aaccatactg ccattgacgg gataccggta
    17281 taccgcgagg agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgtgccagt
    17341 caggttaatg gcagtaacat ttatgccttc cgcgccaatt gttcgtatcg tcgccacttc
    17401 gagagctggt ttcatgctga cggtgccgct ccgggaacta tccatgagat ggagtcttat
    17461 cacggaatgt tggcctgtgt agtcgcagga gcaggcattg cgcttattcc gcgctctatg
    17521 ctggaaagta tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt
    17581 tggttaacaa cctggctggt ctggcgtcgt ggtgcgaaaa cacgtccgct cgaggcattt
    17641 attcaactgc tggatgtgcc tgactcggca agacagggat atcaatgagc tatttttgat
    17701 agttcttgcg gctaatatgc tctatatagt gatgttccga tgatttatga ctatatgggg
    17761 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
    17821 tacgtaatat tgcaccgcag gtaaaagaag ttttaaagga ttttattgat gcacttagta
    17881 caataatatg taatgaagag tggcgcacgt ctttaaacat caactcagcc acaaaaaaga
    17941 tatttaataa ccttgacaac ttatcatata ttcagaggac ttcttttcgg ggtaacgaca
    18001 cgctatacaa tgaaaaggtt cagtttaaac tcacttatcc cgccaggaat ggaagacaca
    18061 aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta gataatttcc
    18121 gccatgatgg agaaattaat attttttgcg ctcccaaccc aaagcctgtc actatggggc
    18181 gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt tctgaatgat tttattgaac
    18241 aatttccaat gatcaaccct ggcgttccca tcaaaagagc gcatacacca catattgaac
    18301 ccctgcctcc ggatcaccat accgctgcag attacttacg ccagtttgat ttgcttgtcc
    18361 tgaattttat ctctcgcggt aattttgtca tactcccccg attatggaat aactctgagg
    18421 ttcacagatg gttcgtcaat aaagatccta acttgatcac cgccattctc gacataacgg
    18481 acagcgaatt aaaagaggat ttattgcaaa gcctgatgga ttcattaggt tctaacaaac
    18541 atgtactacc cgaagtctgc atctgctttt tatccctttt agcagaacaa gaacctcctc
    18601 attttcagga cttgttttta tttttcgcca atatgttgtt acactatcac caatttatga
    18661 atcccaatga aagtgatttg aatgacgtgt tgatgccagc atcattaagt gatgataaaa
    18721 ttatcaaaca tatggcgcgc aagaccctca aactttttgt aaaaaatgaa acaccgccaa
    18781 aagttactca tgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga ccacctatac
    18841 ccgcaacctc caaaacgcca gacattcctg atcgtcatta aaccgtgatg tttaccgtct
    18901 ctctgacgcg tgaaagaatc agcgtcagag aaacggaaaa cgcgatccag atcacaaatg
    18961 cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg caactcaccc
    19021 gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta
    19081 cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt ggcctgctct
    19141 ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcattatg ttgaccattt
    19201 acgcatgggt agtagcgcta atgtcattgc cttttatgct aatgaccagc caggttgaac
    19261 ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta ctgtcgtttt
    19321 tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt gcacatgcga
    19381 ttttctggtc gattacggcg tccctggcga tccgtatggc tccggccggg aagcgagcac
    19441 aggcattgag tttaattgcc acaggtacag cactggcaat ggtattagga ttacctctcg
    19501 ggcgcattgt ggggcagtat ttcggttggc gaatgacctt cttcgcgatt ggtatcgggg
    19561 cgcttattac ccttttgtgc ctgattaagt tacttccctt actgcccagt gagcattccg
    19621 gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc atttatttgt
    19681 taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag ccttttgtgc
    19741 aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta ctcggtggtg
    19801 cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg tctgcgttgg
    19861 taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct gcggcgaaca
    19921 gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg atcatcgggc
    19981 ttggtatgca ggttaaagtg cttgcgctgg caccagatgc caccgacgtc gcgatggcgc
    20041 tattctccgg gatatttaat attggaatcg gggcgggtgc gttggtaggt aatcaggtga
    20101 gtctgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct tttgccgcgt
    20161 taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa cagacgcaat
    20221 agttgaaagg cccattcggg ccttttttaa tgatacgttt taatgatttc caggatgccg
    20281 ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg ggagatcgct
    20341 tcaattcccc ccttgcccac cagccgcata attgcgccag agctgcgtag acttccccac
    20401 aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac ccaatcagca
    20461 aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat ggttcccgga
    20521 ccggcggtac ttggcattgc cagtggtaca aaagcaatat tggcactggg ttcatcttcc
    20581 agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg cggaaagagc
    20641 atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa
    20701 atgccaaatg tatccatcac cagttgcccg gcgtaatacg ccaccatcat gatggcaaag
    20761 acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat gttgcctgcc
    20821 aggccaagaa ataacgcgac agttgttaat gggttagcta acggcagtaa caccaccagc
    20881 cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt gtatctgggt
    20941 tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag ctaaccttgc
    21001 atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatccgtca attcattcat
    21061 ttgacttata cttgcctggg caatattatc ccctgcaact aattacttgc cagggcaact
    21121 aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg cttaatccat
    21181 atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct ggatattacc
    21241 gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa
    21301 ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga tcgcctggtc
    21361 tgtaaaggct gggtggaaag gttgccgaac ccgaatgata agcgcggcgt actggtaaaa
    21421 cttaccacca gcggcgcggc aatatgtgaa caatgccatc aattagttgg ccaggacctg
    21481 catcaagaat taacaaaaaa cctgacggcg gacgaagtgg caacacttga gcatttgctt
    21541 aagaaagtcc tgccgtaaac aaaaaatgag gtatgacgat gtccagacgc aatactgacg
    21601 ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg ccactgtcac
    21661 tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctgcaacgg atgtttaaaa
    21721 aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg gaaatcgcgc
    21781 aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat ggcttcgagt
    21841 cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg cataaatacc
    21901 ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat tacaacagct
    21961 agttgaaaac gtgacaacgt cactgaggca atcatgaaac cacttttatc cgcaatagca
    22021 gctgcgctta ttctcttttc cgcgcagggc tttgcggaac aaacccagca accgctcgtt
    22081 acttcctgtg gcgatgtggt ggttgttccc ccatcgcagg aacaaccacc gttcgattta
    22141 aatcacatgg gtacaggcag tgacaaatcg gatgcgctgg gcgtgcccta ttataaccaa
    22201 caagccatgt agtatattca ggccccggca tatcggggct tgttaacctc ttaccttcac
    22261 cgctttacgc cactgcaaac caaatacatt gatatacagt ccggccataa tgagcacagc
    22321 ccctaagaat tgcagacctg ttaagcgttc atccagcagt aacgctgcac ttgccagacc
    22381 gactaccggc accagtaatg ataatggtgc gacacgccat gtttcatagc gtccaagcag
    22441 cgtaccccaa atcccgtagc caactatcgt cgctacaaat gccagataca tcaacgccag
    22501 aatggtggtc atgtcgatag tgaccagact gtgaatcatt tttccggagc catcgagaat
    22561 gagcgaggcg acaaaaaacg gaattatcgg gattaatgcg ctccagacaa ccagagacat
    22621 taccgccgga cgggatgaat gagacatgat ctttttgttg aagatattgc cactcgccca
    22681 actaaatgct gccgccaggg tcaacataaa gccgagcatc gccacatgct gaccgttcag
    22741 actatcttcg attaacacca ctacgccaaa aatcgctaag gcgatccccg ccaattgttt
    22801 gccatgcagt cgctcaccga aagtaaacgc gccaagcacg atagtaaaaa acgcctgagc
    22861 ctgtaacacc agcgaagcca gtccagcagg cataccaaag ttaatggcgc agaagagaaa
    22921 ggcaaactgc gcaaaactga tggttaatcc ataccccagc agcaaattca gtggtacttt
    22981 cggtcgtgcg acaaaaaaga tggccggaaa agcgaccagc ataaagcgca aaccggccag
    23041 catcagcggt ggcatgttat gaagccccac tttgatgacc acaaaattta gcccccatac
    23101 gaccactacc agtagcgcca acaccccatc ttttcgcgac attctaccgc ctctgaattt
    23161 catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt gattcgctag
    23221 tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat ttttgccatg
    23281 ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac agacaaatga
    23341 acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta ttaaccatcg
    23401 gacgcggcgc tacgctgcca tttatgacca tttacttaag tcgtcagtac agcctgagta
    23461 tcgatctcat tggttatgca atgacaattg cgctcactat tggcgtcgtt tttagcctcg
    23521 gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg gcaattacgg
    23581 ccttcgccag cggttttatt gctattcccc tggtggacag cgtcacgctg gtggtacttt
    23641 ttttcgcact catcaactgc gcctattccg tttttgccac agtgttgaaa gcctggtttg
    23701 ccgacaatct ttcatccacc agcaaaacga agatcttctc gatcaactac accatgctca
    23761 atattggctg gaccgtcggc ccgccactcg gcacgctgtt ggtcatgcag agcatcaatc
    23821 tgcccttttg gctggccgct atctgctctg cgtttcccat gtttttcatt caaatttggg
    23881 taaagcgcag cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg ccgaaagttt
    23941 tattacaaga taaggcactg ttgtggttta cctgctctgg ctttctggct tcttttgtta
    24001 gtggcgcatt tgcttcatgc atttcacaat atgtgatggt gattgctgat ggtgattttg
    24061 ccgaaaaggt ggtcgcggtt gttcttccgg tgaatgccgc catggtagtt acgttgcaat
    24121 attccgtggg ccgccgactt aacccggcta acatccgcgc gctgatgaca gcaggcaccc
    24181 tctgtttcgt catcggtctg gtcggtttta ttttttccgg aaacagcctg ctattgtggg
    24241 gtatgtcagc tgcggtattt actgtcggtg aaataattta tgcgccgggc gagtatatgt
    24301 tgattgacca tattgcgccg ccaggaatga aagccagcta tttttccgcc cagtctttag
    24361 gctggcttgg tgccgcaatt aacccattag tgagtggcct agtactaacc aacctgccgc
    24421 cttcctcgct gtttatcatc ttagcgttgg tgattattgc tgcgtgggta ctgatgttaa
    24481 aagggattcg tgcaaaaccg tgggggcagc ccgcgctttg ttgatttaag tcgaacacaa
    24541 taaagattta attcagcctt cgtttaggtt acctctgcta atatctttct cattgagatg
    24601 aaaattaagg taagcgagga aacacaccac accataaacg gaggcaaata atgctgggta
    24661 atatgaatgt ttttatggcc gtactgggaa taattttatt ttctggtttt ctggccgcgt
    24721 atttcagcca caaatgggat gactaatgaa cggagataat ccctcaccta accggcccct
    24781 tgttacagtt gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac cgccagtaaa
    24841 ccggcggtga atgcttgcat ggatagattt gtgttttgct tttacgctaa caggcatttt
    24901 cctgcactga taacggatcg ttgacacagt agcatcagtt ttctcaataa atgttaaacg
    24961 gagcttaaac tcggttaatc acgttttgtt cgtcaataaa catgcagcga tttcttccgg
    25021 ttagcttacc ctcatacatt gcccggtccg ctcttccaat gaccacatcc agaggctctt
    25081 caggaaatgc gcgactcaca cctgctgtca cggtaatgtt gatatgccct tcagaatgtg
    25141 tgatggcatg gttatcgact aactggcaaa ttctgacacc tgcacgacat gcttcttcat
    25201 cattagccgc tttaacaata atgataaatt cttccccccc gtagcgataa accgtttcgt
    25261 aatcacgcgt ccaactggct aagtaagttg ccagggtgcg taatactaca tcgccgatta
    25321 aatgcccgta ggtatcatta accaatttaa atcggtcaat atccaacaac attaaataaa
    25381 gattcagagg ctcagcgttg cgtaactgat gatcaaagga ttcatcaaga acccgacgcc
    25441 ccggcaatcc cgtcaaaaca tccatattgc tacggatcgt cagcaaataa attttataat
    25501 cggttaatgc cgcagtaaaa gaaagcaacc cctcctgaaa ggcgtcgaaa tgcgcgtcct
    25561 gccagtgatt ttcaacaata gccagcatta attcccgacc acagttatgc atatgttgat
    25621 gggcagaatc cattagccga acgtaaggta attcatcgtt atcgagtggc cccagatgat
    25681 caatccaccg accaaactgg cacagtccat aagaatggtt atccgttatt tctggcttac
    25741 tggcatctct cgcgaccacg ctgtgaaaca tactcaccag ccactggtag tgggcatcga
    25801 tagccttatt gagatttaac aagatggcat caatttccgt tgtcttcttg atcattgcca
    25861 ctcctttttc acagttcctt gtgtgcgcta ttctaacgag agaaaagcaa aattacgtca
    25921 atattttcat agaaatccga agttatgagt catttctgag ataacattgt gatttaaaac
    25981 aaaatcagcg gataaaaaag tgtttaattc tgtaaattac ctctgcatta tcgtaaataa
    26041 aaggatgaca aatagcataa cccaataccc taatggccca gtagttcagg ccatcaggct
    26101 aatttatttt tatttctgca aatgagtgac ccgaacgacg gccggcgcgc ttttcttatc
    26161 gagactgccg ctaatgttga tcatctggtc cggctgaact tctcgtccat caaagacggc
    26221 cgcaggaata acaacattaa tttcaccgct cttatcgcga aaaacgtaac ggtcctctcc
    26281 tttgtgagaa atcaaattac cgcgtagtga aaccgaagcg ccatcgtgca tggtttttgc
    26341 gaaatcaacg gtcatttttt tgcatcatcg gtgccgcgat agccatcttc tattgcatga
    26401 ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt catctgccaa cgcataaggc
    26461 atgacaagaa aacttgctaa tacaatggcc tgaaatttca tactaactcc ttaattgcgt
    26521 ttggtttgac ttattaagtc tggttgctat ttttataatt gccaaataag aatactgcca
    26581 attgttataa ggcatttaaa atcagccaac tagctgtcaa atattcagag aatttaactc
    26641 actaaagtta agaagattga aaagtcttaa acatattttc agaataatcg gatttatatg
    26701 tttggaaatt attatattgg acgagcatac agaaaaagca aatcaccttt acatataaaa
    26761 gcgtggacaa aaaacagtga acattaatag aaataaaatt gtacaacttg cagataccga
    26821 cactattgaa aacctgacat ccgcgttgag tcaaagactt atcgcggatc aattacgctt
    26881 aactacagcc gaatcatgca ccggcggtaa gttggctagc gccctgtgtg cagctgaaga
    26941 tacacccaaa ttttacggtg caggctttgt tactttcacc gatcaggcaa agatgaaaat
    27001 cctcagcgta agccagcaat ctcttgaacg atattctgcg gtgagtgaga aagtggcagc
    27061 agaaatggca accggtgcca tagagcgtgc ggatgctgat gtcagtattg ccattaccgg
    27121 ctacggcgga ccggagggcg gtgaagatgg tacgccagcg ggtaccgtct ggttcgcgtg
    27181 gcatattaaa ggccagaact acactgcggt tatgcatttt gctggcgact gcgaaacggt
    27241 attagcttta gcggtgaggt ttgccctcgc ccagctgctg cagttactgc tataaccagg
    27301 ctggcctggc gatatctcca ggacagccat tggtggtgtt tatatgttca agccacgatg
    27361 ttgcagcatc ggcataatct gaggagcctt accgcgccat tgtcgataca ggcgttccag
    27421 atcttcgctg ttacctctgg aaaggatcgc ctcgcgaaaa cgcagcccat tttcacgcgt
    27481 taatccgccc tgctcaacaa accactggta accatcatcg gccaacattt gcgtccacag
    27541 ataagcgtaa taacctgcgg catatccgcc accaaaaata tgggcaaaat aactgctgcg
    27601 atagcgtggc ggtatagcag gaagatccat attttctgcc accagcgccc gcaattcaaa
    27661 atcatcgaca tcctgcattg cttcgttttc ttccaggcaa tgccagcgca tatcgagaag
    27721 tgcggcgcta agcagttcgc tcatctcata ccctttgttg aacaggctgg cattacgcat
    27781 tttctgttgc agttcgtcag gcattgctgc cccgctctga taatgccggg cgtagcgagc
    27841 gaatacctgc ggatgcgttg cccagtgttc gttgatttgc gacggaaatt cgacaaaatc
    27901 acgcggcgtg ttggtaccgg agagcgtggc aaaacgctgg cgggcaaaaa ggccgtgcag
    27961 cgtatgacca aattcatgga ataaggttat gacatcatcc cagagtaaca acgcaggctc
    28021 accggcagcg ggtttctgat aattgcagac gttataaatt accggatgcg tttcattaag
    28081 cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg ctttttgaat cacgggcgaa
    28141 gaaatcaccg taaaataacg ccagccccac gccattatga tcaaaaattt cccacacccg
    28201 aacgtcagga tggtagacag gaatatcaaa acgttcgaca aacttaatac cgaagagctg
    28261 attcgcggtc cagaatacac cttcattcaa caccgtgttt aattcaaaat atggcttgag
    28321 ctgcgcctca tcaagatcaa atttctctcg ccggacctgt tcggcataaa atgcccagtc
    28381 ccacggctgc gcgctaaacc cgccctgctg cttatcaata accgcctgta tggaggctaa
    28441 ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc cgcataaaat tgagtgctgc
    28501 ttcaggtgtt tttgccatct gatcggcgat tttccatgcg gcataatgag gaaaaccaag
    28561 tagtgttgcc tgttgtgcac ggatctccac cagacgttga atgatagcgc gggtatcatt
    28621 ggcatcattt ttttccgctc gcgtccagcc cgcaataaac agtttttcac gcgtcgcacg
    28681 atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc agcagcggaa tcagccattt
    28741 gttatccaga cctttctcgc gagccgcctc tgccgccagc gcaatctctt gctcactcat
    28801 tcctgccagc tgcgcgatat cgttcacaac cagaccgccg gatttatttg ctgccagtaa
    28861 tcgctggtta aactggctgg tcagggtcgc agcttctgta ttcagtactt ttaattttgc
    28921 tttatcagct tgtgcaagtt tggctccggc taggacaaaa cgttgatgaa tcacctccac
    28981 caggcggatg gattcactat caagccccag agattcacgg cgttgccaga cagcatctac
    29041 ccgcgcgaat aattcaccgt tcagatagat atcattagcc agttccgcca gttcagcgga
    29101 aaactgctcg tcaagacgct gtaattcatc attggtatgc gccgcagtca tcgcaaaaaa
    29161 gacgctggta acgcgggtaa gtaattctcc gctttgttcc agtgccagaa tagtattatt
    29221 gaaatcaggt gtttgcgggt taagcgcgat ggcagcaatt tctgcccgct tttgcagcat
    29281 tccctcatcg aatgccgggc gatagtgatg attggcaatt tgatcaaaat ggggagccag
    29341 atacggcagt gtgctttgca caaggaaagg attcattgtt gtcattttct tctcctgaac
    29401 gcgaggtgtt ccatagcgta ggcttactga taacggagtg caatcttgca aaccagtatt
    29461 acccgctctt aagcatcacg tgctatgtta ttgacacaca aaagcgttga ggaacagtga
    29521 gatgatcgtt ttagtaactg gagcaacggc aggttttggt gaatgcatta ctcgtcgttt
    29581 tattcaacaa gggcataaag ttatcgccac tggccgtcgc caggaacggt tgcaggagtt
    29641 aaaagacgaa ctgggagata atctgtatat cgcccaactg gacgttcgca accgcgccgc
    29701 tattgaagag atgctggcat cgctccctgc cgagtggagc aatattgata tcctggtaaa
    29761 caatgctggc ctggcgttgg gcatggagcc tgcgcataaa gccagcgttg aagactggga
    29821 gacgatgatt gataccaaca acaaaggcct ggtatatatg acgcgcgccg tcttaccggg
    29881 tatggttgaa cgtaatcatg gtcatattat taacattggc tcaacggcag gtagctggcc
    29941 gtatgccggt ggtaacgttt acggtgcgac gaaagcgttt gttcgtcagt ttagcctgaa
    30001 tctgcgtacg gatctgcatg gtacggcggt gcgcgtcacc gacatcgaac cgggtctggt
    30061 gggtggcacc gagttttcca atgtccgctt taaaggcgat gacggtaaag cggaaaaaac
    30121 ctatcaaaat accgttgcat taacgccaga agatgtcagc gaagccgtct ggtgggtgtc
    30181 aacgctgcct gctcacgtca atatcaatac cctggaaatg atgccggtta cccaaagcta
    30241 tgccgggctg aatgtccacc gtcagtaatt tttatacccg gcgtaactgc cgggttattg
    30301 cttgtcataa aaaagtggta gactcatgca gttaactcac tcacaagcaa gaacgaatga
    30361 ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca gcagatttat cgtatccttc
    30421 gtcgcgacat tgtccattgc ctgattgccc caggcacacc gttgtcggaa aaagaagttt
    30481 ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc ctttattaaa ctggcggaaa
    30541 acggcctgat tcaaattcgt ccgcagcgtg gcagctacgt caacaaaatt tccatggccc
    30601 aggtgcgcaa cggcagtttt atccgtcagg ccattgagtg cgcggtggcg cgtcgggcgg
    30661 cgagcatgat taccgagagc cagtgctacc aactggaaca aaatcttcac cagcaacgca
    30721 ttgccattga gcgcaagcaa ctggatgatt tttttgaact tgatgacaac ttccatcaac
    30781 tcctgacgca gattgccgac tgtcaactgg cgtgggatac cattgagaac ctgaaagcga
    30841 ccgttgatcg cgtgcgctat atgagtttcg accacgtttc tccaccagaa atgctgttac
    30901 gccagcatct tgatattttc tctgccctgc aaaaacgtga tggcgatgcg gtagaacgtg
    30961 caatgacgca acatttgcag gaaatcagcg aatccgtgcg ccagatccgc caggaaaaca
    31021 gcgactggtt tagcgaagag taattcattt cctctcatcc catccgggat gagagtcttt
    31081 acccccgcct tatggctcat gcatgcatca aaaaagatgt gagcttgatc aaaaacaaaa
    31141 aatatttcac tcgacaggag tatttatatt gcgcccgtta cgtgggcttc gactgtaaat
    31201 cagaaaggag aaaacaccta tgacgaccta cgatcgtaac cgtaacgcaa tcaccactgg
    31261 cagccgtgtt atggttagcg gcaccggtca cactggcaag atcctgtcga ttgatactga
    31321 aggtctgacc gctgagcaaa tccgccgcgg aaaaaccgta gttgttgaag gttgtgaaga
    31381 gaaactggca cctctggacc tgattcgtct cggcatgaac taagcgtgtg aatgccgccg
    31441 atggcggcat tgcttttttt acttcacgga atatttcgcc acggtcgctt tcgcgccatg
    31501 cgctaataaa gacaagtacg cttccgtcac ttttgcagta aacaagctat tgtctggcaa
    31561 atcaccacca aaaatcgcct taatcgccag caatgactgg acgcgcgctt tcccttcggc
    31621 actactttgt acagccttct gaataacagg taacagtggg tcactgattt ctatcggatt
    31681 tccctgttca tcaacaccac cgacataacg catccaaccc gcgacgccca gcgccagcag
    31741 atcgaacttg ctgtcatgcg ccagatgcca gcgaacagaa tccaacatcc gctgtggcaa
    31801 tttctggctg ccatccatcg caatctgcca ggttcgatga cgtaacgccg ggttgctata
    31861 gcgttcaatt aatcggttag cgtaatcttg caaatcaacg ccctgcactt tcagcgtcgg
    31921 cgcttgttcc tgcaacatca agccatacgc cgcataacga taatgttcat cttccataca
    31981 gtcattaatg tgctgatatc cggcaagata acccaaatac gccaggaatg aatgactgcc
    32041 gttgagcatg cgcaacttca tctcttcata aggcagcaca tcactaacca gttcggctcc
    32101 cgctttttcc cattccggac gtccggcaac aaagttatct tcgattaccc actggcggaa
    32161 aggttcacag gcaacgccag caggatcgcg cacaccggta agctgttcga ttttcgccag
    32221 cgtatcctct gtcactgcgg gcacaatacg gtccaccatt gttgatggga aagtcacgtt
    32281 atcttcgatc cattgtgcca gttttacatc aacggcttgt gcgtatgaag tgacaacgtc
    32341 acgcataaca tgaccgtttt ctggcatgtt gtcacatgac atgacggtaa atgcgggaag
    32401 tcctgccgct ttacggcgag ccagcgcctc aacaatcacc cctgttgctg ttttcggctg
    32461 gtggggattt tgcacgtcgg cagcgaccat cgggtgatcg agcattaact gtccggtcgc
    32521 cggagagtgg aaataccctt tttcggtgat tgtcagagag acaatcgcga tttgcggttc
    32581 acacatcgca gccaacacgg tttctaagcc atccatctgt acgtgcaagg cttttttaac
    32641 gacgccaacg acgcgtgccg tccacgcatc ggccgacatt tccgcaacgg tataaagatt
    32701 atcttgctgt tgtaaatcgg caatttgctg ttcgccaccg attaagttga cctcataata
    32761 tccccagtca ctgaaatgtt ctgtagcaag aatatcggca tacacgccct gatgcgcacg
    32821 gtgaaatgca ccaaagccta aatgaacaat tcttggggcc aggttattac gatcataaac
    32881 aggaagcgtc gcttttgctg ataacaaatt atttcccata actattcctt aaatataaat
    32941 atggcaagct acatgttttg ttatatgaat aaaaaatccc ctctccggta agagaaggga
    33001 ttgaggattt aaagacttct ggaaggttgc gcagctctta caacacgcgg ttgatcttct
    33061 gcagcgtctt ccagcgcact taaatcacgg tctttcactt ctggcatttt cagcgcagag
    33121 attaaaccaa tcactgaata tgccatgatc ataatggcga tcggatacca ggattccgtc
    33181 atggtgcaga aaatacccgc caggatagga ccaaaaccgg aagcgataag accaccaatt
    33241 tctttagaaa tagccatccg ggtaaagcgg tttttacagc cgaacatttc tgccatggta
    33301 atgttttcca gagcaaataa tcccagcacc gcacagttat gaatcacaat cagtgcaacc
    33361 ataatggtgc ttggggcata gcttttatct acgatgatag aaagcattgg ccacgccagt
    33421 acaatcgcag aggtattcat aataatatac gggatccggc gaccaatttt atcggataac
    33481 caaccaagga acggaatggt cataaagccg agaatcgaac tgatcatcaa tgcatctgtt
    33541 ggaattgctt tgttaaacaa taacgtctgt actaaatagc ctgcaaggaa agtctgaatt
    33601 aaaccggagt tacccgcctg accaaaacgc agccctgttg ccagccagaa ggatttgctc
    33661 tggaacatgc taccagcagg tgcaggtttt gctgtcggtt ggttgctgtc attaaccttc
    33721 tcaaagaccg ggctttcttt cagattcata cgtaaccaga tagcaaagac catcacgaca
    33781 acgctcgcca ggaacggtat acgccatccc cacgccagca gttcctcttt actgagaatg
    33841 aagaacataa aggcccagat tgccgttgcg ctcaaggttc cgcagttagt tcccatagct
    33901 acaaatgagg agataattcc gcgcttacct tttggcgcat attccgccag catcgtaccg
    33961 gcaccggaaa tgtccgcacc tgcacccaac c
//
LOCUS       DAZUGK010000029        33159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZUGK010000029 DAZUGK010000000
VERSION     DAZUGK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.029"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1380
                     /gene="argA"
                     /locus_tag="ACHY5W_001241"
     CDS             49..1380
                     /gene="argA"
                     /locus_tag="ACHY5W_001241"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFS4446171.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1442..3268)
                     /gene="recD"
                     /locus_tag="ACHY5W_001242"
     CDS             complement(1442..3268)
                     /gene="recD"
                     /locus_tag="ACHY5W_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFS4446172.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSSLGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(3268..6810)
                     /gene="recB"
                     /locus_tag="ACHY5W_001243"
     CDS             complement(3268..6810)
                     /gene="recB"
                     /locus_tag="ACHY5W_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFS4446173.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVLTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKRVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(6803..9691)
                     /gene="ptrA"
                     /locus_tag="ACHY5W_001244"
     CDS             complement(6803..9691)
                     /gene="ptrA"
                     /locus_tag="ACHY5W_001244"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFS4446174.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAANTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLSVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9867..13235)
                     /gene="recC"
                     /locus_tag="ACHY5W_001245"
     CDS             complement(9867..13235)
                     /gene="recC"
                     /locus_tag="ACHY5W_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFS4446175.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWEKQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13248..13571)
                     /locus_tag="ACHY5W_001246"
     CDS             complement(13248..13571)
                     /locus_tag="ACHY5W_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFS4446176.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(13537..13962)
                     /locus_tag="ACHY5W_001247"
     CDS             complement(13537..13962)
                     /locus_tag="ACHY5W_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFS4446177.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKEGRYVSFPEESTRL"
     gene            complement(13959..14522)
                     /locus_tag="ACHY5W_001248"
     CDS             complement(13959..14522)
                     /locus_tag="ACHY5W_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS4446178.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(14513..14983)
                     /locus_tag="ACHY5W_001249"
     CDS             complement(14513..14983)
                     /locus_tag="ACHY5W_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS4446179.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(15168..15962)
                     /gene="thyA"
                     /locus_tag="ACHY5W_001250"
     CDS             complement(15168..15962)
                     /gene="thyA"
                     /locus_tag="ACHY5W_001250"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4446180.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(15969..16844)
                     /gene="lgt"
                     /locus_tag="ACHY5W_001251"
     CDS             complement(15969..16844)
                     /gene="lgt"
                     /locus_tag="ACHY5W_001251"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4446181.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(16995..19241)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001252"
     CDS             complement(16995..19241)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001252"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4446182.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19254..19784)
                     /gene="rppH"
                     /locus_tag="ACHY5W_001253"
     CDS             complement(19254..19784)
                     /gene="rppH"
                     /locus_tag="ACHY5W_001253"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFS4446183.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(20137..20283)
                     /gene="ygdT"
                     /locus_tag="ACHY5W_001254"
     CDS             complement(20137..20283)
                     /gene="ygdT"
                     /locus_tag="ACHY5W_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFS4446184.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            20469..21158
                     /gene="mutH"
                     /locus_tag="ACHY5W_001255"
     CDS             20469..21158
                     /gene="mutH"
                     /locus_tag="ACHY5W_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFS4446185.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            21227..21940
                     /locus_tag="ACHY5W_001256"
     CDS             21227..21940
                     /locus_tag="ACHY5W_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS4446186.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            22078..22296
                     /gene="ygdR"
                     /locus_tag="ACHY5W_001257"
     CDS             22078..22296
                     /gene="ygdR"
                     /locus_tag="ACHY5W_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFS4446187.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            22404..23444
                     /locus_tag="ACHY5W_001258"
     CDS             22404..23444
                     /locus_tag="ACHY5W_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFS4446188.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(23476..24669)
                     /gene="lplT"
                     /locus_tag="ACHY5W_001259"
     CDS             complement(23476..24669)
                     /gene="lplT"
                     /locus_tag="ACHY5W_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFS4446189.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSTMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(24662..26821)
                     /gene="aas"
                     /locus_tag="ACHY5W_001260"
     CDS             complement(24662..26821)
                     /gene="aas"
                     /locus_tag="ACHY5W_001260"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFS4446190.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHVLPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVKIQGRAKRFAKIAGEMV
                     SLEMVEQLALSVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            27406..28437
                     /gene="galR"
                     /locus_tag="ACHY5W_001261"
     CDS             27406..28437
                     /gene="galR"
                     /locus_tag="ACHY5W_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFS4446191.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(28444..29706)
                     /gene="lysA"
                     /locus_tag="ACHY5W_001262"
     CDS             complement(28444..29706)
                     /gene="lysA"
                     /locus_tag="ACHY5W_001262"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4446192.1"
                     /translation="MPHSLFSTDTALTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            29828..30763
                     /gene="lysR"
                     /locus_tag="ACHY5W_001263"
     CDS             29828..30763
                     /gene="lysR"
                     /locus_tag="ACHY5W_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFS4446193.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAIKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(30750..31442)
                     /gene="ygeA"
                     /locus_tag="ACHY5W_001264"
     CDS             complement(30750..31442)
                     /gene="ygeA"
                     /locus_tag="ACHY5W_001264"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFS4446194.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINDGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(31571..32989)
                     /gene="araE"
                     /locus_tag="ACHY5W_001265"
     CDS             complement(31571..32989)
                     /gene="araE"
                     /locus_tag="ACHY5W_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFS4446195.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8122 a         9107 c         8242 g         7688 t
ORIGIN      
        1 cagaataaaa atacaataat ttcgaataat catgcaaaga ggtgtgccgt ggtaaaggaa
       61 cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
      121 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
      181 atcgttaatg atatcgggtt attgcacagc ctcggcatcc gtctggtggt ggtctatggc
      241 gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct gtatcacaag
      301 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
      361 caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct acagggcgcg
      421 catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
      481 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat ccatcgtcaa
      541 ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
      601 tttaacctga cctcggaaga gattgccact caactggcca tcaaactgaa agctgagaag
      661 atgattggtt tttgctcttc acagggcgtc actaatgacg acggtgatat tgtctccgaa
      721 cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg cgattacaac
      781 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgtcgc
      841 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
      901 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
      961 gatatcggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
     1021 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
     1081 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
     1141 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
     1201 attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac cacgcgcagt
     1261 attcactggt tccaggaacg tggatttacc ccagtggata ttgatttact gcccgagagc
     1321 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttagggtaa
     1381 gcgatgaaaa tcgccggatg cggcaggcgt aacactcgtg cgtcgcatcc ggcaattacg
     1441 tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta cgagtggcga
     1501 ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca
     1561 ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
     1621 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtagtttcgt
     1681 gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc gcaaaccaga
     1741 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
     1801 agctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag tgcggattac
     1861 gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt ccagccacgc
     1921 caaatggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc tgaatgatta
     1981 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc gcaagagctt
     2041 cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag ccgtttttcg atatcagtaa
     2101 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
     2161 actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg cagagactgt
     2221 cgcgcaaaga tgccgcttct gtgccagttc ctgccggaac gtgagtcccc gtcaggcggc
     2281 ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag atatcgccca
     2341 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
     2401 catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
     2461 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
     2521 tacccggctg tgcacccagc aatcggtgca aagtactggc atcttccgga atgcgtttct
     2581 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
     2641 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
     2701 gaattaacgc tgccagcaac ttcgctacgg tggtcgtttt accggtgcca gggccgccgg
     2761 aaatcaccga gatccgccgc gtcagcgcca ctgccgccgc aactttttgc cagttaattt
     2821 catcgcttac tggaaaaagt ttgtccaagg tttgcgccag tagagcttca tcaacctcaa
     2881 tggcatgatt cacttcgttg aaaaagcgtg ccaccgtacg ttcgttacac cacatgcgat
     2941 tcaaataaag acgatcgcca cagaggatca tcggcgttgg ttcatctccc cggctgaccg
     3001 cttgtgaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
     3061 tcgccaacag cggatgcgac gcctcattat tttccagtcg tgaaagcggc aaacagacgt
     3121 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggcg ggatgttcat
     3181 ctcccgccac agtcagggca aactgcacgt ccagcgggcg tagctgtttg tgctccacag
     3241 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
     3301 ctcatccatc aggtcaatca acccggcgtt ggggcgggtt gtgtaaatcc cttgttgcgg
     3361 atgttcttta tcaacaccac gcaggaacag ataaataacg ccgccaaagt ggcgctcata
     3421 gtcgtaatca gcaatgcgat ggcggagata acggtgcagc gccagggtat aaagctgata
     3481 ttgcagatca tagcggtgtg cctgcattgc cgctgccata gcctgttggg tgtaagccga
     3541 actgtcttca cccaaccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
     3601 aaacaccagg tcgataaagc cttttaacat gccacgtacc tgcatgaact ccagcggcgg
     3661 gcagccagcg gatagcgggt caaactggcg gattaacgta tcaagctgac tggcgataag
     3721 cggttcacta atcggcagat aaaactccat ctccacccgt ttattgcggg cggaaagttg
     3781 actcaggctt acgccggttt cattgagagg tgcctggagg acagccgtga tccactcggt
     3841 caataccggt tcccactgcg attcaaagcc gccgagttcc agtttttccc gcacccagtt
     3901 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg
     3961 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
     4021 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
     4081 ctgcaaaccg gagtagctgg tgacgcgcca gttatcgccg ggcaatcgtt gtaacgtctt
     4141 cgcattcagt tctgcagtta aaacatcatt aacctgccaa ggctggttat caccagtttg
     4201 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
     4261 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
     4321 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
     4381 aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcggcctc
     4441 cgcgagatcg acactttctg gcgcagcgtt aagatccaga acagcctcaa acgagtggcg
     4501 atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca accagacaag
     4561 cggatattcc agcccttttg atttatggat cgtgacaatc tgcaccagat gtttatcact
     4621 ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
     4681 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa gcagttcgct
     4741 gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttctgc
     4801 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccaaat
     4861 ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt cattgttcag
     4921 cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac tgcgcagggt
     4981 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
     5041 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
     5101 ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacactga tgtccgaagc
     5161 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
     5221 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat aatcgccaac
     5281 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
     5341 gacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
     5401 aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca ccattccagg
     5461 tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac gcgccttcat
     5521 ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt cgccaatcag
     5581 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
     5641 gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc
     5701 caacacctca ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
     5761 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
     5821 gcgggtgatc accagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc
     5881 aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta agaaacgctg
     5941 ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt ctgcccaggc
     6001 gctgatcttg tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
     6061 agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct gttttaccgt
     6121 atcaatacgc gccacaattt gcgcgtgacg ggaagccagc gtttcatcat cgggcggcgg
     6181 tgctttgata accggcgctt caccttgcag ataacgatta atatcgcgca gcaacgcctg
     6241 cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg ggtagcagtg
     6301 gcgacgccag aaatcggcgc aggcttggta gcgtagcaga gactcatctt caatcagctg
     6361 ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct ggcaaaagcc
     6421 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
     6481 ggcttgcgct ttatcgtcga tctcttccag cagacgttcg tacagtggat tgtcggtggt
     6541 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cgcgcaattc
     6601 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca gcgggcgggg
     6661 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
     6721 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
     6781 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
     6841 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
     6901 cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg accaccgcct
     6961 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
     7021 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
     7081 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
     7141 actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
     7201 gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
     7261 gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct tcggtacgca
     7321 actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag gctgagctgg
     7381 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
     7441 cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg tttcgacacc
     7501 actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggg
     7561 cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
     7621 gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt tcatctcgcg
     7681 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca aacgctttac
     7741 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt
     7801 ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc ggcagacgct
     7861 gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa aaacttatgc
     7921 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
     7981 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
     8041 tgacatcagc tttgggttcg ctggaaaaat aacggcttgg cgcatacacc acgcgcagat
     8101 tcgactcatc aacaatcagc tctggatggt catatttctt ctctgactta atcagcgaga
     8161 aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatattg gcagcttttt
     8221 gctgccagtc agcgaaagtt tgttcgctga ttttatcgac ctgatacggc gcatcgacaa
     8281 aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
     8341 gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg gcaatattga
     8401 ctgcatccag cgtatgctca accggaacgc gaatcatggt atctgccagc cattcgacgt
     8461 aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca ttagccagtt
     8521 catcgaagta ttgtttatcg atcccttttt cacgtaacaa attgaggtag ctaaaaatag
     8581 ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca gagatcgcta
     8641 atacaccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
     8701 cctgcttttg cagccagtca gacagtgtac ctgggctgcg attaccaatc agataggtaa
     8761 tcaattcatc cgttttactg cggaacttcg ctgagttgtt atcgatgcga aactcaacgc
     8821 tcaacacttt acgcggcagc gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg
     8881 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
     8941 tgttcgccgc catttttgcc aactccggca gcggtttatt actgtaaata accgccttca
     9001 tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
     9061 caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
     9121 ggttaatggt ttctgcgctg acttgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
     9181 ttaattcggc attcaccgca ttacgctcac gttcggcgta tttcttgtcg agcaagggtt
     9241 ctgcaatagc gtccgccagg cggtctaccg caccgggcaa ggcgtcgttc tcaacttcca
     9301 gatagaaagc cgtgcgatac ggtgccgtgc tggcattgtg gctgccaccg tgcattttga
     9361 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
     9421 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacca
     9481 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
     9541 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
     9601 gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccaaagggca actaacaaca
     9661 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa ctttattttc
     9721 tggccagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
     9781 gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga
     9841 tagattgcgc aatttttata cagcgctcat gactgattaa agcgaaacag cggtaacagg
     9901 aaacgttgcg actgttcaac gattgcctcc attgtctctg gtgttaattg ccgccagagc
     9961 ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    10021 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    10081 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    10141 gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    10201 gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta
    10261 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt
    10321 aataaagagg gacgccagcg caacaagcca tccggctgca cctgcggcaa ccagccagtt
    10381 atctgcacac cgttgcaagc gagatcaatt tccatactct gccccggctg gcgacaggca
    10441 atgactctgt cggcaagctg ctgcatctcc tggcactgtt tttcccagaa aatttcacca
    10501 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    10561 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagtccttcc
    10621 agaataaatg gctcggtgtc ggggatttcg ctgtcttcag tacggaagtt cacctgcaaa
    10681 cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    10741 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    10801 tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt
    10861 tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg
    10921 ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc gatgtagtcg
    10981 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    11041 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa cagatagcgg
    11101 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    11161 aatggcgcaa gctggcgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    11221 aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    11281 tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag tgacagcggc
    11341 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    11401 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    11461 cgacaaaccg gcaaccactc ttccagcgga cgctcctgtg ccagcccgcg acgccagatg
    11521 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    11581 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccaac
    11641 aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag ttccagctcg
    11701 cgaacgttgt cgtcatctat cccccaacga atgccggact cgttgaccca ctggcgtaaa
    11761 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagcagc
    11821 gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaagct gataaacgct
    11881 tccagtaccg gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg caggtaacga
    11941 tccgcaggtg cgctaccaaa cactgcctga ataaacggac tgtagctgtc gatgtcggcc
    12001 accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cctccagcat cgccagcagg
    12061 cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg
    12121 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    12181 ccagcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta
    12241 tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc agaaaggaga
    12301 taaatgtagt cgcgcccaag cttaccccat gaggccagca gcgggttgcc gacatcctgt
    12361 tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cgcgaaatag cggcaattca
    12421 cgatcttcaa aactgtgtcg acgctggcgg gtcagcagtt tcgccagata agcaggatct
    12481 ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcgatatgt
    12541 ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    12601 aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    12661 cgctgataga gattggcgcg gtgccaacgc ggttgtccga gttcatgtgt atattccacc
    12721 agcgccttcc acaacggcgc ttgccaggcc tgtgcctctc ccagcccttc aaccaaatgt
    12781 cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc
    12841 gccgcttttg aggaaagctg gaacagcttt cgcttgtcgc tatcgtcagt cagataatgc
    12901 cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat cagtttccag
    12961 ctcatgctct gtttgttaaa ggcgctctct ttggggatct ccggtaacac ccggacgaac
    13021 atatcccaga taaagctcgc aggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    13081 tgcgacaggg tcatttgcag ccactgtgcc ataccggtac tttgcaccag aatcatctct
    13141 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    13201 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    13261 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    13321 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    13381 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    13441 ctgctggcaa gactgttcat taatgttcgc tggaaacccg ataacgcagt gacaatcatc
    13501 accatcagca ccatcgccaa cattacctcc ggcaggctaa agccttgttg attcttcagg
    13561 gaagctgaca taacgcccct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa
    13621 aacaatgttt ccatcgatga cttcacctgt tcgccacaac gaaacgcctt cataaccggc
    13681 aatcaataag gcttcattat ctgccaataa acgcaaacaa acccgggcat cggttccagc
    13741 gtactgcgag cactgaactg ctggctgcgt ctgccaggag tgcatttttc cccacgccag
    13801 cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaggc
    13861 aaaactgcga tcctgttggc tcattccttg taatagcaag ctacccaaaa ctagcagcat
    13921 caggaccaga gccagtgacg aaacaccctt ttcacggttc acaggttgaa tcctgtcacg
    13981 ctatagctgg cattcaccac ggtttgcggt tcagacttac tggcagcacg catattaacc
    14041 gtcaacaccg gcgagaagcc gctgacatcc tgacgtacta cctgaaaagt gtcgataatg
    14101 atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt
    14161 agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcggactc ttttaccggt
    14221 tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt
    14281 ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga
    14341 tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg
    14401 cccgttaaac tttcacgctg taacgcaggc agaaagcgtg ccgctcccag caacaatacg
    14461 ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc
    14521 atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc
    14581 accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc
    14641 caaagaaggc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa
    14701 agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc
    14761 ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccaa ttggcatctt
    14821 cacgtaaata gagcaaatag tctcgcgcct ggctggcggt ttgccatagc cgttgcgact
    14881 gctgccagta ttgccagcca tagagtccac ttgcacttag catgaccaaa atcagcatcg
    14941 cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagca
    15001 aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac
    15061 gccgttgcag gacgttgcaa aatttcggga aggtgtctcg aagaatttaa tggagggtaa
    15121 aaaaaaccga cgcacactag cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    15181 ggcgctttaa tgcccggatg cggatcgtag ccttcaatct caaagtcttc gaaacggtag
    15241 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    15301 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgtgtatc gccaccggtc
    15361 cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg
    15421 tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctga
    15481 caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc
    15541 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg
    15601 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc tggcgttggc
    15661 caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg
    15721 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    15781 cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    15841 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    15901 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    15961 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    16021 acacccgcga caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag
    16081 gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    16141 tagccaatca ggaacaagcc tgagacagct cccattgggc gcggtttacg aatatacagg
    16201 ttgaggataa taaacagcac cacaccttcc agcagcagtt cgtaaagctg tgatgggtgg
    16261 cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc
    16321 aaaatatctt ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg
    16381 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    16441 atgagtggtg cgataaaatc agaaacctgg aagaaggaac gtttagtacg gcgggcgaag
    16501 ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc gccgtcccag
    16561 acacggaaca gatacagcgg atcagccata aactgcggga aattgtagaa cagaacataa
    16621 ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    16681 tttttggtcc agccgctgcc cggacgattc gcccgacgtg ttgccagcca cattgcaaaa
    16741 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    16801 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    16861 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    16921 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    16981 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata
    17041 aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca
    17101 agattttctg cttcggcata atcaatgcga cgcagcaggt attttacccg cgctacagaa
    17161 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    17221 tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg
    17281 atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg
    17341 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    17401 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg
    17461 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    17521 cgttcaatca ggcggcgtgc ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga
    17581 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    17641 atctccggct gatcgagcgt aatgcgaatc ccacgccacc ccaggcatgg attctcttcg
    17701 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    17761 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    17821 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    17881 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    17941 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    18001 tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc
    18061 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    18121 atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgattgc agcatgggag
    18181 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    18241 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    18301 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcg
    18361 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    18421 gtttttaccg cccattctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    18481 agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    18541 gcaccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    18601 ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    18661 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    18721 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa
    18781 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    18841 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    18901 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    18961 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    19021 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    19081 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    19141 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    19201 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    19261 cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc
    19321 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    19381 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    19441 gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt
    19501 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    19561 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    19621 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttaattcc gccttgcgga
    19681 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    19741 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaatat
    19801 aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca
    19861 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    19921 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    19981 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat
    20041 attcattatc agtcaatgca ttacatgtaa tttaagtaat gaaaaagagt aattcgtgac
    20101 ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    20161 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    20221 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    20281 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacccgt tgcagtgttg
    20341 cgcaactcga ttaccggcaa cctaaaaagc aatataaatc agagaattga acaacgcatg
    20401 tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt
    20461 atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt
    20521 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    20581 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    20641 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    20701 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    20761 ccccgttaac gggcaatagc ggagtgacct gggaaaccag ccacgtgcgc cacaaactca
    20821 aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg
    20881 ttggatcacc gttgctgtgg agcccgaatg aagaggaaga ccggcagcta cgcgaagact
    20941 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggattacc gctcgtcacg
    21001 gggagtattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    21061 gtgcccgggg cgaacggatt ctgacgctgc cgcgcggctt ttatttgaag aaaaatttca
    21121 ccagtgcgct gctggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    21181 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    21241 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    21301 tgggatcgac aatattattt tcctttctct ggtggtggca aagctcccca cagcacaacg
    21361 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    21421 atcaatcgcc tgggttacgc gcctgacgaa tccgcttttt acaatattca gtcaggaaat
    21481 ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    21541 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    21601 cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat
    21661 taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg taattgccgt
    21721 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgagcgcc atccttcggt
    21781 aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt
    21841 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    21901 aagcctcaac ctgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc
    21961 gggagagcgt actcactcct gttaccctca cagtttaaga ttttcctgct ttcaagatat
    22021 ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga taaccggatg
    22081 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    22141 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    22201 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    22261 aaccgtgatg atgtttcgca aataattgaa cgttaacaaa taaggtcagc atccggctgg
    22321 ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc
    22381 gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag
    22441 tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc
    22501 acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt
    22561 acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc
    22621 tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc
    22681 gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag
    22741 cgctgcatga cagcctcaag cgcctacaga ctgattacct cgatctttat caggtgcact
    22801 ggccgcagcg cccaaccaac tgcttcggca aactcggtta tagctggaca gattctgcgc
    22861 ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa
    22921 ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac ctgcatctgg
    22981 cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccttacagt ctgttaaacc
    23041 gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct
    23101 attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca aaacccgctg
    23161 gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag
    23221 ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggatcctgct cagatggcgc
    23281 tcgcttttgt acgtcgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg
    23341 agcagctgaa aactaacgtc gaaagtttgc atctggagtt gagcgaagac gtattagctg
    23401 aaattgaagc ggtgcatcag gtttatacgt atcctgcacc ataagattaa cgccggttaa
    23461 aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc
    23521 aaatagcgca ccgaagccaa tgccaatggg cacgactggg atgcctacca ttaccgccag
    23581 cgagtaaatg cccagcatca acaacatggt gctgttttcg ccaaggtttt gtactgcaat
    23641 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
    23701 gacaaaaaag ccccccagca cgccaatcag catcaacaag gcataggctg gcagcagctc
    23761 gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
    23821 tgacacggtt tccagcgtaa ccagcttcgc tgccgcacct gcgccaacca cgataccaat
    23881 cgctaccatc gcgttgagat aggtcggcgt agcgttatcg gtaatgccca gcgccaccgg
    23941 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
    24001 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
    24061 gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccgcca gtttgggaat
    24121 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gggcgaccag
    24181 gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg ccgctatggt
    24241 agaagcttcc attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag
    24301 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    24361 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    24421 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
    24481 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    24541 cgctttcagt aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa
    24601 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    24661 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    24721 taccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    24781 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    24841 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    24901 ctttatctgg cgaaacacta agtgccagtt gttccaccat ttccagcgac accatttcgc
    24961 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctt cacaaagccc tgctcgtcaa
    25021 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    25081 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    25141 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
    25201 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    25261 aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg ccaaatttat
    25321 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    25381 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtggaggtac
    25441 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
    25501 ggcttggata aaggaacact tctgcacctg tgagcagtgg cgtaaacagg cctaccgtca
    25561 gcccaaagga gtgaaacagc ggcaacgccg acataaagcg atcgttggtg gtgaagtcgg
    25621 caatcgtttt aatctgctcg acattcgcca gaatgctttt atggctatgg acgacgcctt
    25681 tcggatggcc ttcagaaccg gaggtaaaaa ggatcagcgc ctcttcttcc ggctgctgtt
    25741 ttacctgtgc cagacgcggc atcagcaaat gagcgaagat ccacactttg tcggcagtgg
    25801 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    25861 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    25921 ccgccgtaat ggcgctggtc agccctttta ccccagcggt gtagttcatc attgcgggaa
    25981 tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    26041 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    26101 ttttagtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    26161 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagcgtt tcacgcgggc
    26221 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    26281 ggtcacgggc acgcggcgca tccggcatct ccacctgcgt tggtggcaaa acatgcagag
    26341 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    26401 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaatcccg
    26461 cgccatcgta gattttcatc agcgagccag tggtggtgat tcgtccttca gggaaaatca
    26521 ccactggtcg gccctgttcc accagacgta ccagatgttt aatcgccata ggttgcgtcg
    26581 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    26641 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    26701 caataaaaga gacgtgatta ggcgtgatta gaacgcgctc gcccttcagt gcctgggtgt
    26761 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    26821 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    26881 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    26941 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    27001 tgattgtggt ctgcacgacg acccttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    27061 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaacc
    27121 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    27181 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    27241 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt
    27301 gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc
    27361 cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag
    27421 gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca
    27481 cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc tcttagctat
    27541 cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctggtc
    27601 gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct
    27661 tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt
    27721 caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg
    27781 atcccagatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctaatcaac
    27841 cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta cggtgcctgg
    27901 ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac
    27961 cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt
    28021 ggtattccgg ccaatgatcg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa
    28081 caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac
    28141 gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggc
    28201 gaaatttcgt taattggctt tgatgatgta ctggtgtcac gctatgtgcg cccgcgcctg
    28261 accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact ggctttggcg
    28321 ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt
    28381 cgccattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg
    28441 cagttaaagc aattccagcg ccaataattc ttcgatggtc tggcgacggc gaatcaaccg
    28501 cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt agttggatga
    28561 cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc
    28621 cggcaaggcg cgggtttcga cattcccccc ttcctgctgg gtaaagacat cgcccgattc
    28681 acataacggc ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc
    28741 agctgccaga gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt
    28801 gaacccggca tcaacaagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg
    28861 agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt ccagtttcac
    28921 agggtggccc aaatggcggg caatttgctc acgcgcggca ttccacagac cataataatg
    28981 ttcggtatca accgcctctt caccctgttg ataaggaaca gaaagcccac cgcccgcaga
    29041 aatagcctgt aaatcctgac cgaattcgag gacctgacgc accatcgcac cacacacctg
    29101 ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcag
    29161 atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt
    29221 gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccgggttaac
    29281 gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca tatcaacaga
    29341 acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac
    29401 atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc
    29461 acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg
    29521 caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag
    29581 cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc
    29641 agcgggcaaa cgcagcagat tttcggcggt gagagcggta tcggtgctga acagtgaatg
    29701 tggcatagca aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata
    29761 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    29821 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    29881 cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga
    29941 actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac gtggacgatt
    30001 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    30061 ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    30121 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    30181 acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg
    30241 gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac
    30301 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct
    30361 ggcgataaaa aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc
    30421 ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg
    30481 acgcatgatc gtagaaaccc acagcgccgc gtcagtgtgc gcaatggtac gggcgggggt
    30541 aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    30601 gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc
    30661 gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcgggtttac cgaaactggt
    30721 cacttctctt gacgctattc tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    30781 gcatggatcg ccgcggtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa
    30841 ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc
    30901 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    30961 ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat
    31021 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc
    31081 gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag
    31141 aaaggcagag agcaacgaga ctctatagca tccgccactt tgtgcatcgt attggtacat
    31201 aacacaatgc cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatatcc
    31261 ccggttttgt cccactcgcc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta
    31321 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc atcatttatc
    31381 agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    31441 attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc
    31501 tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg
    31561 gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga
    31621 tatgttccag ggtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    31681 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgctg tcgagcagcg
    31741 tcaggaaggt cgcgccgata atcatattcg acacccagtt cgtggtggtc gaacaggtaa
    31801 taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg
    31861 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    31921 agccactgga agccgtaccg ttatcaaact gcatcaggca gtaacccagc accagagtgc
    31981 ctaacgccat cacgctgaaa ccaattttca gagccggctt acgcccggct ttatctaccg
    32041 taaacaccgc aataaaagta gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca
    32101 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg tagtacatga
    32161 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac
    32221 gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt
    32281 cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca
    32341 cttcttccgc ctcaatatga cgcccctttt ccgctagcca gcgcgggcta tttggcagga
    32401 agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc
    32461 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga
    32521 ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    32581 gatacagagg agcggtgtaa gaggcgatcc cgacagcaat gcccagcacc acacgagcgg
    32641 cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga
    32701 tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca
    32761 gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc aaacgactgg
    32821 tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac
    32881 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    32941 cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    33001 agcagagagt aagacatagt gaaaaaatac gtgaacaact cacacaggtg tcaggtcgga
    33061 aacagcataa atatggatta aattgctgcg acatgtcgtt atgtgatgga tattccaatt
    33121 ttcaaattaa gttgaattat tgagattatt attaaccac
//
LOCUS       DAZUGK010000030        32556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZUGK010000030 DAZUGK010000000
VERSION     DAZUGK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.030"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(404..490)
                     /locus_tag="ACHY5W_001266"
     tRNA            complement(404..490)
                     /locus_tag="ACHY5W_001266"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Leu,seq:gag)
     gene            complement(505..837)
                     /gene="secG"
                     /locus_tag="ACHY5W_001267"
     CDS             complement(505..837)
                     /gene="secG"
                     /locus_tag="ACHY5W_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4446196.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1065..2402)
                     /gene="glmM"
                     /locus_tag="ACHY5W_001268"
     CDS             complement(1065..2402)
                     /gene="glmM"
                     /locus_tag="ACHY5W_001268"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4446197.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2395..3243)
                     /gene="folP"
                     /locus_tag="ACHY5W_001269"
     CDS             complement(2395..3243)
                     /gene="folP"
                     /locus_tag="ACHY5W_001269"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFS4446198.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATLIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3333..5267)
                     /gene="ftsH"
                     /locus_tag="ACHY5W_001270"
     CDS             complement(3333..5267)
                     /gene="ftsH"
                     /locus_tag="ACHY5W_001270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4446199.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5367..5996)
                     /gene="rlmE"
                     /locus_tag="ACHY5W_001271"
     CDS             complement(5367..5996)
                     /gene="rlmE"
                     /locus_tag="ACHY5W_001271"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFS4446200.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6122..6415
                     /gene="yhbY"
                     /locus_tag="ACHY5W_001272"
     CDS             6122..6415
                     /gene="yhbY"
                     /locus_tag="ACHY5W_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4446201.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6571..7047)
                     /gene="greA"
                     /locus_tag="ACHY5W_001273"
     CDS             complement(6571..7047)
                     /gene="greA"
                     /locus_tag="ACHY5W_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4446202.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVLKVEYL"
     gene            7295..8728
                     /gene="dacB"
                     /locus_tag="ACHY5W_001274"
     CDS             7295..8728
                     /gene="dacB"
                     /locus_tag="ACHY5W_001274"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS4446203.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEQLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNESGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8769..9941)
                     /gene="cgtA"
                     /locus_tag="ACHY5W_001275"
     CDS             complement(8769..9941)
                     /gene="cgtA"
                     /locus_tag="ACHY5W_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFS4446204.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9957..10922)
                     /gene="yhbE"
                     /locus_tag="ACHY5W_001276"
     CDS             complement(9957..10922)
                     /gene="yhbE"
                     /locus_tag="ACHY5W_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4446205.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTPVVSQPRAGE"
     gene            complement(11049..11306)
                     /gene="rpmA"
                     /locus_tag="ACHY5W_001277"
     CDS             complement(11049..11306)
                     /gene="rpmA"
                     /locus_tag="ACHY5W_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4446206.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11327..11638)
                     /gene="rplU"
                     /locus_tag="ACHY5W_001278"
     CDS             complement(11327..11638)
                     /gene="rplU"
                     /locus_tag="ACHY5W_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4446207.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11897..12868
                     /gene="ispB"
                     /locus_tag="ACHY5W_001279"
     CDS             11897..12868
                     /gene="ispB"
                     /locus_tag="ACHY5W_001279"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFS4446208.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13096..13374
                     /gene="sfsB"
                     /locus_tag="ACHY5W_001280"
     CDS             13096..13374
                     /gene="sfsB"
                     /locus_tag="ACHY5W_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFS4446209.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13422..14681)
                     /gene="murA"
                     /locus_tag="ACHY5W_001281"
     CDS             complement(13422..14681)
                     /gene="murA"
                     /locus_tag="ACHY5W_001281"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4446210.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14736..14990)
                     /gene="ibaG"
                     /locus_tag="ACHY5W_001282"
     CDS             complement(14736..14990)
                     /gene="ibaG"
                     /locus_tag="ACHY5W_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFS4446211.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15150..15443)
                     /gene="mlaB"
                     /locus_tag="ACHY5W_001283"
     CDS             complement(15150..15443)
                     /gene="mlaB"
                     /locus_tag="ACHY5W_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFS4446212.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15443..16078)
                     /gene="mlaC"
                     /locus_tag="ACHY5W_001284"
     CDS             complement(15443..16078)
                     /gene="mlaC"
                     /locus_tag="ACHY5W_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFS4446213.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTTQLKSISQQKITLEEKK"
     gene            complement(16097..16648)
                     /gene="mlaD"
                     /locus_tag="ACHY5W_001285"
     CDS             complement(16097..16648)
                     /gene="mlaD"
                     /locus_tag="ACHY5W_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFS4446214.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16653..17435)
                     /gene="mlaE"
                     /locus_tag="ACHY5W_001286"
     CDS             complement(16653..17435)
                     /gene="mlaE"
                     /locus_tag="ACHY5W_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFS4446215.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17443..18252)
                     /gene="mlaF"
                     /locus_tag="ACHY5W_001287"
     CDS             complement(17443..18252)
                     /gene="mlaF"
                     /locus_tag="ACHY5W_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFS4446216.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18462..19439
                     /gene="yrbG"
                     /locus_tag="ACHY5W_001288"
     CDS             18462..19439
                     /gene="yrbG"
                     /locus_tag="ACHY5W_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFS4446217.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19453..20439
                     /gene="kdsD"
                     /locus_tag="ACHY5W_001289"
     CDS             19453..20439
                     /gene="kdsD"
                     /locus_tag="ACHY5W_001289"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFS4446218.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20460..21026
                     /gene="kdsC"
                     /locus_tag="ACHY5W_001290"
     CDS             20460..21026
                     /gene="kdsC"
                     /locus_tag="ACHY5W_001290"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFS4446219.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21023..21598
                     /gene="lptC"
                     /locus_tag="ACHY5W_001291"
     CDS             21023..21598
                     /gene="lptC"
                     /locus_tag="ACHY5W_001291"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFS4446220.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21567..22124
                     /gene="lptA"
                     /locus_tag="ACHY5W_001292"
     CDS             21567..22124
                     /gene="lptA"
                     /locus_tag="ACHY5W_001292"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFS4446221.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22131..22856
                     /gene="lptB"
                     /locus_tag="ACHY5W_001293"
     CDS             22131..22856
                     /gene="lptB"
                     /locus_tag="ACHY5W_001293"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFS4446222.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22904..24337
                     /gene="rpoN"
                     /locus_tag="ACHY5W_001294"
     CDS             22904..24337
                     /gene="rpoN"
                     /locus_tag="ACHY5W_001294"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4446223.1"
                     /translation="MKQGLQLRLSQQLTMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24360..24647
                     /gene="hpf"
                     /locus_tag="ACHY5W_001295"
     CDS             24360..24647
                     /gene="hpf"
                     /locus_tag="ACHY5W_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFS4446224.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24765..25256
                     /gene="ptsN"
                     /locus_tag="ACHY5W_001296"
     CDS             24765..25256
                     /gene="ptsN"
                     /locus_tag="ACHY5W_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFS4446225.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQAKTHMHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25302..26156
                     /gene="rapZ"
                     /locus_tag="ACHY5W_001297"
     CDS             25302..26156
                     /gene="rapZ"
                     /locus_tag="ACHY5W_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4446226.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26153..26425
                     /gene="npr"
                     /locus_tag="ACHY5W_001298"
     CDS             26153..26425
                     /gene="npr"
                     /locus_tag="ACHY5W_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFS4446227.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26638..27270
                     /gene="yrbL"
                     /locus_tag="ACHY5W_001299"
     CDS             26638..27270
                     /gene="yrbL"
                     /locus_tag="ACHY5W_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFS4446228.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27267..27995)
                     /gene="mtgA"
                     /locus_tag="ACHY5W_001300"
     CDS             complement(27267..27995)
                     /gene="mtgA"
                     /locus_tag="ACHY5W_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFS4446229.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27992..28645)
                     /gene="elbB"
                     /locus_tag="ACHY5W_001301"
     CDS             complement(27992..28645)
                     /gene="elbB"
                     /locus_tag="ACHY5W_001301"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFS4446230.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28875..31211)
                     /gene="arcB"
                     /locus_tag="ACHY5W_001302"
     CDS             complement(28875..31211)
                     /gene="arcB"
                     /locus_tag="ACHY5W_001302"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFS4446231.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31307..32236)
                     /gene="yhcC"
                     /locus_tag="ACHY5W_001303"
     CDS             complement(31307..32236)
                     /gene="yhcC"
                     /locus_tag="ACHY5W_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4446232.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWIPPTE"
BASE COUNT         8078 a         8843 c         7854 g         7781 t
ORIGIN      
        1 tatcagacat gtttttcaac tctgatccat atcagaacga aacactgtta gttttttctt
       61 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
      121 acattacgta gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
      181 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
      241 tcagcagtag ggaaaagctt ataacgaagg cgtaccggca ccatcgtttc aaggtatcag
      301 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaagac
      361 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
      421 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
      481 ccacctcggc acggaaacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
      541 agctggctga gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt cgctaccttt
      601 attggtcttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
      661 cagcaacgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
      721 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
      781 cagaccaaca aggccaattg ccacaataag gaaaactacc aaaagagctt cgtacataat
      841 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
      901 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
      961 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga
     1021 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
     1081 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
     1141 acgcgaatta acggttcggt gccggattta cgcaacagca cacgtccacg gctacccagc
     1201 gccgcttcaa cttctgcggt caccgctttg actgattcat gctccagcgg atcgccgcta
     1261 cctgcggtgt aacgcacgtt taccaggatt tgcgggaaca ttttcatgcc gctgcaaagg
     1321 tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaaacc agcaacgatg
     1381 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata
     1441 cgccagcctt tttcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
     1501 aatggaatac ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
     1561 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
     1621 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
     1681 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
     1741 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
     1801 acattcgctc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
     1861 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
     1921 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
     1981 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
     2041 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgcgat
     2101 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
     2161 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
     2221 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
     2281 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
     2341 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
     2401 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
     2461 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
     2521 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
     2581 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
     2641 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
     2701 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
     2761 cacttctgca aagacatcgt catacttcgg ggcttcctgc atggtttttg gatttccctg
     2821 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
     2881 ggaaagggag cggatatcgt taataatgtg agcgccaact ttcgctgact cacggatgac
     2941 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
     3001 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctggccgcgt
     3061 ggactcgcca ccaacgtcaa tgagcgtcgc gccagcgttg atcatcagat tcgcatgttt
     3121 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
     3181 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
     3241 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
     3301 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
     3361 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
     3421 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
     3481 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
     3541 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
     3601 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
     3661 tgtttcgctt tcgctacgct acggccgagg aacacttcac cttcttcttc cgcgtacagc
     3721 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
     3781 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct
     3841 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
     3901 ctgattgcgt caccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
     3961 atcgtcactt tgtgcaccgg atcgtgctcc ggcaccaggc gaccgataat cgcatgaccc
     4021 gcttcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
     4081 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
     4141 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
     4201 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
     4261 cgcatgtgaa ccttcagtat ctgctcacga ccgcgaacat ctggcaagcc aaccacaacc
     4321 tgacggtcga aacggccagg acgcagcagc gctgggtcga gaacgtccgg acggttagtc
     4381 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
     4441 ttcagagtct gttcacgttc atcgtgacca ccgcccagac ctgcgccacg ctggcggcct
     4501 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
     4561 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
     4621 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcag cgttttaccg
     4681 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
     4741 cggctcggct cgcgcagata ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag
     4801 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
     4861 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
     4921 caaacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
     4981 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
     5041 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
     5101 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
     5161 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
     5221 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
     5281 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
     5341 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
     5401 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
     5461 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
     5521 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
     5581 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
     5641 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
     5701 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
     5761 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
     5821 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
     5881 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
     5941 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
     6001 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
     6061 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
     6121 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
     6181 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
     6241 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
     6301 cttgatcgtg gaagctatcg ttcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
     6361 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
     6421 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
     6481 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
     6541 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa gtacttcaaa
     6601 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
     6661 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
     6721 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
     6781 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
     6841 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
     6901 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
     6961 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
     7021 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
     7081 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
     7141 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
     7201 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
     7261 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
     7321 attgaccagc tgtatagcgt ttagtgttca ggccgcaaat gttgatgagt acattactca
     7381 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
     7441 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
     7501 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
     7561 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
     7621 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
     7681 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
     7741 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
     7801 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
     7861 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
     7921 tggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
     7981 tacgctgacg ggatgcctgc cacaacgttc tgagcagctc ccgttggctt ttgccgtgca
     8041 ggatggagcc agctatgccg gtgcaattct gaaagatgag ttaaaacagg caggtatcac
     8101 ctggagcgga acactgctgc gccagactca ggttaacgaa tctggaacgg tagttgccag
     8161 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
     8221 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
     8281 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
     8341 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
     8401 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
     8461 gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
     8521 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
     8581 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
     8641 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
     8701 gtataaagat atttatcaga ataattagtc taaagagaaa ccccggcaca tggccggggt
     8761 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
     8821 tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca
     8881 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
     8941 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc actcgccgca
     9001 gagatcagat aatatttatc ttcccagccc agcgcctcag cgatcgcttt cgctttctct
     9061 tcggcttcta ccttatccag cagatcgatc ttgttgaaca ctaaccaacg cggtttcgcc
     9121 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
     9181 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
     9241 tgcttcagga agcgaatgcc cagacctgcg ccttccgcag cgccttcaat cagtcctgga
     9301 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc
     9361 agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc acgaataaag
     9421 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
     9481 agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
     9541 actgacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
     9601 cgctgaccgt gtttggtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
     9661 gtacctaccg gcactttaat cgtgacgtct ttaccgcgct taccggtaca gtcgcggctt
     9721 gcgccgttct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
     9781 ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccatc gccgccatcc
     9841 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
     9901 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat
     9961 tcgcctgcgc ggggttgcga taccaccggt gtatgcttac gtaatccgcc ccaaatacga
    10021 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    10081 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    10141 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
    10201 gccatcgctt ccgccagggc gccatatcct accaaggtat tcagtccgca aaaaattaaa
    10261 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    10321 gcaattgtac ataaagtata cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
    10381 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg cagcaccgac cccaaagata
    10441 actccccagg tgtaatcggt tagctttgta aatatctcga ccagactggt gttaaaaaac
    10501 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    10561 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    10621 gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc
    10681 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    10741 ggcaacctct tcttcaccgc aagaatggcc cccaggccaa tactcgccat caagaaacgg
    10801 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
    10861 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    10921 ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc
    10981 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    11041 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    11101 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    11161 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    11221 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    11281 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    11341 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
    11401 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    11461 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    11521 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    11581 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    11641 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    11701 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    11761 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
    11821 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    11881 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    11941 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
    12001 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    12061 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    12121 tatccacacg gcgactctgc tacacgacga cgttgtggat gaatcagata tgcgcagggg
    12181 taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat
    12241 ttatacccgc gctttccaga tgatgaccag cctcggttcg ctcaaagtgc tggaagtcat
    12301 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga atgttaacga
    12361 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    12421 tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg
    12481 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    12541 ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga acgaaggtaa
    12601 accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat
    12661 ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat
    12721 gaacgcttgt ggatctcttg aatggacgcg tcagcgcgcc gaggaagaag cagacaaagc
    12781 catcgcagcg ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc
    12841 gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgca gtgcgcggga
    12901 tgattccagt aaactccata ataatactta ttcagcgtaa taatgcctga aaaaccggtg
    12961 tatacatctt tatatattct gaatattcac actctttaca ggaacttttt agagcaatcg
    13021 gccatcagga gtatagtgat gctcgacaga agaagtgttc tgaatgaaag cgactaactt
    13081 aaggagtgag gaaagatgga aagtaatttc attgactggc atcccgctga catcattgcg
    13141 ggtttgcgca agaagggaac gtcaatggcg gcggaatctc gcagaaatgg tttgagttcc
    13201 tcaacgctgg cgaatgcatt atcgcgccca tggccgaaag gagaaatgat tattgcgaaa
    13261 gccctgggaa ctgacccctg ggttatctgg ccatcacgct accatgatcc gcagacccat
    13321 gagtttatcg acagaacgca gttgatgcgt agctacacta aaccgaaaaa atgagtggtc
    13381 tggcggtagc cccgcgaacg gggctgccag ctctcagacg attactcgcc tttcacacgc
    13441 tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg
    13501 tgataaatac gatcaaccac cgtcgtccct tccgcaatac agccagccag caccaggctt
    13561 gctgatgcac gcagatcggt tgccataacc tgtgcgccag aaagtttttc aacaccgtga
    13621 caaataacgg tattgctttc gatttcggcg tgtgcgccca tacggctcag ctctggcaca
    13681 tgcataaaac ggttttcaaa gaccgtttcg gtgatgaacc cggtcccttc tgccaccagg
    13741 ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt
    13801 acgttaacag ccttcggacg tttgccatgc atatccaggc taatccagtc ttcgccgact
    13861 tcgatgtccg ctccagcgtc acgcagtttc gccagcacgg cgtcgagagt atctggctgc
    13921 gcgttacggc agataatttt gccgcgagaa atcgctgccg ccaccaggaa agtaccggtt
    13981 tcgatacgat ccggtagcac gcgatagaca ccgccgccta aacgttccac accttcgatg
    14041 acgatacgat cggtgccctg accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg
    14101 gtatcgacga tttccggttc acgcgctgcg ttttcaataa tcgtggtgcc ttccgccaga
    14161 gttgcagcac acatgatggt caccgttgcg ccaacgctga ctttatccat cacgatatgt
    14221 gcgcctttca gacgaccatc gacggaagct ttaacgtaac cttcttccag tttgatggtc
    14281 gcgcctaatt gttcgaggcc agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa
    14341 ccgccaggta gtgaaacttg cccctgacca aagcgcgcta ccagcggccc cagtgcccag
    14401 atagaagcac gcatggtttt aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg
    14461 gcatcaatat gcacagaacc attacgttct actttcgcac ccagctggct tagcagcttc
    14521 attgatgtat cgacgtcttt cagtttcggg acgttctgga tctctaccgg ttcttccgcc
    14581 agtagagcgg caaaaaggat aggcagagca gcatttttag cgccggaaat tgtgacttcg
    14641 ccctggagct tcgttggccc ctgaacacga aatttatcca tttagtttgt tctcagttaa
    14701 caattcatat ccgctaccgg cgaatcgccc atagctcaaa agccgttcag tttgcgatcg
    14761 cgcgcccact ccgcaggggt atacgctttg atcgacacag catgaatgcg gttatccgca
    14821 atatattcca tcagcggacc atagaccgtc tgctgttttt taacccgact catgccgtca
    14881 aacaactcac ccacggcaat aacctgaaag tggctgccat cgccggaaac gtggacttcc
    14941 tggagggaga gagcgttcat caacacgctc tgaatttcat tattttccat gggatcttca
    15001 atcatcagtt aataaaccag cgaaacatct tagagcaaag ttgcgctggc ataaataagc
    15061 aaaaagcctc gctgataaat cagacaaggc tcgacttgca ggcaggtttg ccggacaggc
    15121 ggttaacgcc atatccggcc tgaaaaaatt taacgaggca gaacatcagc aggcaaatta
    15181 tacaatttcg ccaaggtata cactttgtcg tttaccccct gaagcgtcac attgttgccc
    15241 tgctttttcg ccagatcgat aagatggagc agcagtgcca gtccccccgt atccacgcgg
    15301 gacacacggc taagatcgat gcaggtaacc cccttcaccg cttcctcacg catttcccaa
    15361 agcggtagca gaacgtcctg atccagctct ccggataacg ccagcgtgtc acccgtctgc
    15421 atccagctca gtgactcgct cattattttt tctcttccag agtgattttc tgttgagaaa
    15481 tcgatttcag ttgcgtagtc aggccgtcga tacctttggt acgcagcagc gttccccact
    15541 cgttttgttt ggtggtgatc atactgacgc cttcagcaat catgtcgtaa gcctgccagt
    15601 ttcccgtctg ggagttttta cgccactgga agtccagacg caccggcggg cggccattcg
    15661 ggtcaataat ggtaacgcga ataggcacaa tggttttatc gcccagcggc tgttctggcg
    15721 caatctgata ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt
    15781 actcacggaa agcggcaaag taagcatcac gttgagctgg ggtcgcactc ttgtaatact
    15841 ggcccagcac cagcgcaccg gcgtatttca cctgtacgta tggcagcagt tcctgatcaa
    15901 caatggtacg cagataatcc gggttggccc gaatttgcgg ttgctcattc ttcaggcgat
    15961 cgaacgtttt ctgcgccgcc tcgtccatca gcttatacgg attggtctgg tctgccgcgg
    16021 ttgccgcact cagcggtgca atcaccagca aagcgaccat cattaaacgt ttaaacatgc
    16081 gtcggttctc ctgaaattat ttcgttgtac ccacaggttc agtggtttca ttattacctg
    16141 gcgcagcagc tggcgcatcg ccactattct tattgtcatc gcctttacta ccgtaaagga
    16201 actgaccaat gagatcttcc agcaccatcg cagacttggt gtcctgaatt gtatcgccat
    16261 ccttcaggat agcagtcccc agttccgggt cttcaaaacc gacgtttaat gccagatatt
    16321 gttctcccag caggccggaa gtacgaatgc tcagcgaact ggtatctgga atgtggttat
    16381 aacgttgttc aatttccagc gttacacgtg gcagataggt tttcgggtcc agcgtaatat
    16441 ccgccacccg gcccacaaca acaccaccaa tgctgaccgg agagcgcgct ttcaggccgc
    16501 caatgttatc gaacgtcgca taaagcgtgt aggtcggttc agtacgtatt gacgtcacgt
    16561 tcgccgcctt caggcaaaca aacagcgccg ccagcagtgc tgctaataaa aagataccca
    16621 cccaaatttc attttttttc gtttgcatga actcaattcc caaacatcaa tgcggtcagc
    16681 acaaaatcca gccccagaac agccagagac gagtggacaa cggtgcgagt ggttgcccgg
    16741 ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc
    16801 gtgatggcga acaccacgct cttaatcaga cagttgacca gatccatacg ccagtcgacg
    16861 gcattttgca ttgccgacca gaagaacccg ctatcaatgc ctttccaact gacgccgacc
    16921 agcgatccgc cccagatccc cacggcgacg aagataaccg tcaacagtgg taatgaaata
    16981 accccagccc agaaacgggg agaaatgacc cgacgcagcg gatccaccgc catcatctcc
    17041 atactggaga gttgctctgt agcgcgcatc aggccgattt ctgcggttaa cgccgaacca
    17101 gcacgcccgg caaacaacag cgcggcaacc accggcccca gttcacgcag tagcgataac
    17161 gccaccagca tacccagact ggtttccgca ctataagtgg tcagaaccag ataaccttgc
    17221 agccccaaca ccattccgat gaacacgcca gaaactacaa taatcagcat cgacaggacg
    17281 ccgacattat agagctgacg caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg
    17341 accagcgcat tgaataacat taacccggcc cgcccgaacg ttctcagggt tttaatccct
    17401 ttatgtccga gcgacgccag cgcatttaac agcatgagtg gcttaactcc ctggtaaaag
    17461 atcagcgtga taatcgccgg caggatagcg gaacggaaca ggcccgtcag ctatcccgtc
    17521 cagaaactga cgtacgcgcg gatcaggatt cgcctgcaac gcctgggcac tgccatgagc
    17581 gacaattttt ttgtccgcca ggatccaggc gtgatccgca atacttaaca cttccggcac
    17641 atcgtgagaa accaccacac aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt
    17701 caccagtacg cccatggtaa tgggatcttg cccaacaaaa ggttcatcaa acatgatgag
    17761 atccggctcc agcgcaatcg cacgtgccag cgctgcacgc cgcgccatcc caccggaaag
    17821 ttcagaaggc ataagtttag ccgctccacg cagccccacg gcctccagct tcatcatcac
    17881 cgtactatgc aacaatggcg cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt
    17941 gtcaaatacg ttcatatcag tgaacaacgc cccggactga aataacatgc tcatccgttt
    18001 gcgcactgta tacaggcgcg aacgtgacat cgccggaata ttctcaccat cgaaaaggat
    18061 ctcaccatga tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat
    18121 gcccgatggc cccatgatcg ccgtgatctt ccctcgcggc acggtcaggg aaatattatc
    18181 gaagatgcag cgattgccac gcgtaaaact gacatcgcgc atatcgacta aattcgccac
    18241 agactgctcc ataattcacc cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt
    18301 aaccttgaac ccaacatatt tacagaatat tacccgccgt ggttagcgaa agctggcatt
    18361 tgttttactt tttagccgca taaagtcaaa attaagaatc cgttacggct ttctgaaaat
    18421 cttcagcgga ccggcgagta tacccgaaga aaggacgtta gatgctttta gctacggcac
    18481 tgttaattgt tggtttactt ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt
    18541 ctattctttg ccgaaccttt ggcatcccgc cgctgatcat cggcatgacg gtggtcagta
    18601 ttggtacatc gttaccagaa atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg
    18661 atttagccgt tggtacagcc ctcggctcaa acattatcaa tatattgctg atcctcggtc
    18721 tggctgcgct ggttcgtcct tttaccgtcc attctgatgt tctacgccgt gaattaccct
    18781 taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta tgacggacaa cttagtcgca
    18841 gcgatggtat ctttctcctc tttctggctg tgctatggct gctgttcatt gttaaacttg
    18901 cacgtcaggc tgaacgtcag gggactgaca gcctgaccag agagcagctt gcagagctgc
    18961 cgcgtgacgg cggattgccc gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc
    19021 cagtggccac gcggatggtg gttgataacg ccacggtgct ggcgaattac tttgccatca
    19081 gcgagttgac gatggggctg acggcaattg ctatcggaac cagcctgccg gaactggcaa
    19141 ccgcaatcgc gggggttcgc aaaggtgaaa acgacattgc tgtcggaaat atcattggcg
    19201 caaacatttt taatattgtc atcgtgttgg gtttacccgc gctgataacg ccaggagaga
    19261 ttgatccact ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc attatttttg
    19321 cgttgctgtg ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta ttattaactg
    19381 gcggatttat cgtatggctg gcgatgttgt actggttatc gccaatactc gttgaataac
    19441 tggaaacgca ttatgtcgca cgtagagtta caaccgggtt ttgactttca gcaagcaggt
    19501 aaagaagtcc tggcgattga acgtgaatgc ctggcggagc ttgatcaata catcaatcag
    19561 aatttcacgc ttgcctgtga aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg
    19621 atggggaaat cggggcacat cgggcgcaaa atggccgcaa cgtttgccag caccggtaca
    19681 ccttcatttt tcgtccatcc tggtgaagcc gcacatggtg atttaggcat ggtcacccca
    19741 caggatgtgg tgattgctat ctctaactca ggtgaatcca gcgaaatcac tgccttaatt
    19801 ccagtgctta agcgtcttca cgtaccgtta atctgcatca ccggtcgccc ggagagcagc
    19861 atggcgcgcg ccgcagatgt gcatctgtgt gttaaagtag cgaaagaggc ctgtccgtta
    19921 gggctggcac cgaccagcag caccaccgcc acgctggtta tgggcgatgc cctcgctgtc
    19981 gcgctgttaa aagcacgcgg ctttactgct gaagattttg cgctctcaca tccaggcggc
    20041 gcactgggtc gtaaacttct gctgcgcgta aacgatatta tgcatacggg cgatgagatc
    20101 ccgcatgtta agaaaacggc cagtctgcgt gacgcgttac tggaagttac ccgcaaaaat
    20161 cttggtatga ctgtcatttg cgatgacaat atgatgattg aaggcatctt taccgacggt
    20221 gatttacgcc gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg
    20281 atgacgccgg ggggaatacg cgtgcgccct ggcattctgg ccgttgaggc actgaactta
    20341 atgcagtccc gccatatcac ctccgtgatg gttgccgatg gcgaccattt actcggtgtg
    20401 ttacatatgc atgatttact gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa
    20461 tgagcaaagc aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc gacgttatag
    20521 caaaagcaga gaacattcgt ctgctgatcc tcgacgttga tggcgtactg tcagatggcc
    20581 tgatttatat gggcaataac ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg
    20641 gcattcgttg tgcgctcacc tctgatattg aagtcgctat cattaccggg cgaaaggcta
    20701 aactggtaga agatcgttgt gccacattag ggatcactca cttgtatcag gggcagtcaa
    20761 acaaactgat cgcctttagc gatctgctgg aaaaattggc gattgccccg gaaaatgtgg
    20821 cttatgttgg cgatgatctc atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg
    20881 ccgtggccga tgcgcatcca ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg
    20941 gcggtcgtgg cgcagtgcgc gaagtttgcg acttattact cctggcgcag ggcaaactgg
    21001 atgaagccaa agggcaatcg atatgagtaa agccagacgt tgggttatca ttgtgctatc
    21061 actggcggtt ctggtgatga tcggcattaa tatggccgaa aaagacgata ccgcccaggt
    21121 ggtcgtcaac aacaatgatc ccacctataa aagcgagcat acggacacgc tcgtctataa
    21181 cccagaaggg gcactaagct atcgattgat tgctcaacac gttgaatatt attccgatca
    21241 ggccgtttcg tggtttacgc agccggtact taccacgttt gataaggata aaatcccgac
    21301 atggtccgta aaagcagata aagccaagct gaccaatgac cggatgctct atttatatgg
    21361 acacgttgaa gtcaacgcac tcgtgccgga ctctcaactt cgcagaatca cgacggataa
    21421 cgcgcagatc aatctggtga cgcaggatgt tacctctgaa gacctcgtca cgttatacgg
    21481 aacaacattt aactccagcg gtctgaaaat gcgcggcaac ttacgcagca agaacgccga
    21541 gctgattgaa aaggttagaa catcctatga aattcaaaac aaacaaactc agccttaatc
    21601 ttgtgcttgc cagctcactt ctggccgcca gcattccggc atttgccgta accggagaca
    21661 ctgatcagcc gatccacatt gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg
    21721 ttacctttac cggtaatgtc atcgtcaccc agggcaccat caaaattaat gccgacaaag
    21781 tggtcgttac ccgtccgggc ggcgaacaag gtaaagaagt gattgacggc tacggtaaac
    21841 cggcaacgtt ctaccagatg caggacaacg gtaaacccgt tgaaggtcac gcttcccaga
    21901 tgcactacga actggcaaaa gattttgtcg ttctgacggg taatgcttat ctgcagcagg
    21961 tcgatagcaa cattaagggc gataagatca cttacctggt aaaagagcag aaaatgcagg
    22021 ctttcagcga caaaggcaag cgtgtaacca ccgttctggt gccgtcgcag ctgcaggaca
    22081 aaaacaacaa aggccagacc ccggcacaga agaagggtaa ttaattcgtt atggcaacat
    22141 taactgcaaa gaaccttgca aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc
    22201 tgaccgtcaa ctccggggaa attgtcggtc tgctggggcc aaacggtgcc ggtaagacca
    22261 ccactttcta catggttgtt ggcattgtgc cgcgcgatgc gggcaacatc attattgatg
    22321 atgacgatat cagtctgctg cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc
    22381 cacaggaagc ctccattttc cgtcgcctca gcgtttacga taacctgatg gcggtactgc
    22441 aaattcgtga cgacttgtct gctgaacaac gtgaagaccg tgcgaacgag ctgatggaag
    22501 agtttcacat tgagcacctg cgtgacagca tggggcagtc actctccggg ggtgaacgtc
    22561 gccgcgtaga aattgcccgc gcactggctg cgaatccgaa atttattctg ctcgacgaac
    22621 cgtttgccgg ggttgacccg atctcggtta tcgacattaa acgcatcatt gagcacctgc
    22681 gcgacagcgg cctgggcgtg ctgatcactg accacaacgt gcgtgaaaca ctggcggttt
    22741 gtgaacgcgc ttatatcgtc agtcaggggc atttgatcgc ccacggcacg cctacagaaa
    22801 tcttacaaga cgaacacgtt aagcgtgtat accttgggga agacttcaga ctctgatagg
    22861 gtagaagttt gcgacgtttt agcaggagag tacgattctg aacatgaagc aaggtttgca
    22921 actcaggctt agccaacaac tgacgatgac gccacagctt caacaggcaa ttcgtctgtt
    22981 gcagttgtcg acgctggaac ttcagcagga gctacagcag gcgctggaga gtaatccgct
    23041 gcttgagcaa atcgacactc atgaagaaat cgacacccgc gaaacgcaag acagtgaaac
    23101 gctggacacc gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc
    23161 cagttgggac accatttaca ccgctggtac gccatccggc accagcggtg actacattga
    23221 cgatgagctg ccggtctatc agggcgaaac gacgcagacc ttgcaggatt acctgatgtg
    23281 gcaggtcgag ctgacaccgt tttccgacac tgaccgcgct attgctacct ctatcgtcga
    23341 tgccgttgat gacaccggtt atctgactgt cccgctggaa gatattctcg aaagtatggg
    23401 cgatgaagag atcgacatcg acgaagttga agccgtcctt aagcggatcc aacggtttga
    23461 tccggtcggt gtggcggcaa aagatctgcg tgactgcctg ctgatccaac tctcccaatt
    23521 cgataaaacc acgccgtggc tggaagaggc cagactgatc attagcgatc atctcgatct
    23581 gttagccaat cacgacttcc gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct
    23641 gaaagaagcc gtcaatctga tccagtcgct cgatccgcgc cccgggcagt cgatccagac
    23701 tggcgaacct gagtatgtca ttccagatgt gctggtgcgt aagcataacg gtcactggac
    23761 ggtagaactc aacagtgaca gcattccgcg tctgcaaatc aaccagcact acgcctcgat
    23821 gtgcaataac gcgcgcaatg atggtgacag ccagtttatc cgcagcaatc tgcaagatgc
    23881 caaatggttg atcaagagtc tggaaagccg taacgatacg ctactgcgcg tgagtcgctg
    23941 tatcgttgaa cagcagcaag ccttctttga gcaaggtgaa gaatatatga aaccgatggt
    24001 actggccgat atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac
    24061 gcaaaaatac ctgcatagtc cacgaggcat ttttgaactg aagtatttct tttccagtca
    24121 cgtcaatacc gagggcggcg gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa
    24181 attaatcgcg gcggaaaacc cagcgaagcc gttgagcgac agcaagttaa cctctttgct
    24241 gtcggaacaa ggtatcatgg tggcacgccg cactgttgcg aagtaccgag agtctttatc
    24301 cattccgccg tcaaaccagc gtaaacagct cgtttgaccc aaccgataag gaagacacta
    24361 tgcagctcaa cattaccgga aataacgtcg agatcaccga ggcactgcgc gaatttgtta
    24421 cagccaaatt tgccaaactt gagcaatatt ttgaccgaat caaccaggtc tatgttgttc
    24481 tgaaagtgga gaaagtcacc cacacctcag atgcaacact gcatgtaaac ggtggtgaaa
    24541 ttcatgccag cgcggaaggt caggacatgt atgctgccat tgatggctta attgataagc
    24601 tggcacgcca gttgaccaaa cataaagata aactgaaaca acactaattg tccgggcaat
    24661 tagcatgtgc atggcggcct gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc
    24721 gagcctgttc cactgtttga gtgggcaggt tcttaggtga aattatgaca aataatgata
    24781 caactctaca gcttagcagt gttcttaaca gggaatgtac gcgaagccgc gtccactgtc
    24841 agagcaaaaa acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc
    24901 cgcctcaggt ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt accggtatcg
    24961 gcaatggtat tgccattccg catggcaaac tggaagaaga tactctgcgt gccgttggcg
    25021 ttttcgttca gctcgaaacg cctatagctt tcgatgccat cgacaaccaa ccggtggact
    25081 tactttttgc cctgctggtg ccggcagacc aagctaaaac gcacatgcat actctgtcgc
    25141 tggtggcgaa acgtctggcg gacaaaacca tctgccgccg tttacgcgca gcccagagcg
    25201 atgaagagct gtatcaaatc attacggata ccgaaggtac tccggatgaa gcgtagttat
    25261 tcggtaatgt ctcttttaga cgttgtgagg agaaacagta catggtactg atgatcgtca
    25321 gcggacgttc aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat atgggttttt
    25381 actgcgtgga taaccttccc gtagtgttgt tacccgatct ggctcgaacc ctggccgatc
    25441 gcgagatttc tgccgccgtc agcattgatg ttcgtaatat gccggagtca ccagaaatat
    25501 tcgaacaggc gatgagtaac ctgcctgacg ctttctcacc gcaactcctg ttcctggatg
    25561 ccgaccgtaa taccttaatt cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca
    25621 gcaaaaacct gtcgctggaa agtgctatcg acaaagaaag cgatctgctg gagcctctgc
    25681 gttcgcgagc ggatctgatt gtcgatacct cagaaatgtc cgttcacgag ctggcagaaa
    25741 tgctgcgtac ccgtctactg ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt
    25801 tcggcttcaa acacggtatc cctatcgatg cagattacgt ctttgacgtg cgcttcttgc
    25861 cgaacccgca ctgggatccg aaactgcgtc caatgacagg tcttgataag cctgtcgccg
    25921 cgttcctcga ccgccacaca gaagtacaca attttatcta ccagacgcga agctatcttg
    25981 agctatggtt acctatgctg gaaaccaaca accgtagcta cctgacggtc gccattggtt
    26041 gtaccggcgg gaagcaccgt tcggtgtata ttgcagagca actggctgac tacttccgct
    26101 cgcgcggtaa aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt
    26161 caagcaaact gttgaaatca caaacaagct gggcatgcat gcccggcctg caatgaagct
    26221 gtttgaatta atgcagggtt ttgacgctga agtgctgtta cgtaatgacg aaggtaccga
    26281 ggctgaagcc aacagcgtta ttgcgctgct gatgttggat tctgccaaag gacggcagat
    26341 tgaagttgaa gcgaccggtc cacaggaaga ggaagcactg gccgccgtta tcgccctctt
    26401 taactctggt tttgatgaag attaatcttc atcactttga cattcaaatc ccttcaagct
    26461 cccccggcaa taagaaaagc gcgtactgaa atcgttctca atcaacgtca attgtacatt
    26521 tcgtgcgctt tttactttca gaagaacctt aagaaaacct taagaagcat tgtttaggtt
    26581 tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg agatggcatg
    26641 attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg ttatgcgcat
    26701 ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg tggcgataaa
    26761 gagatccgtc gtgagttaaa gtactacgcg catcttggtc gccggttaaa agactggagt
    26821 ggaataccgc gctatcacgg taccgtcgag acggattgcg gaaccggtta tgtttatgat
    26881 gtgattgccg attttgacgg caagccctcc attacgctga ccgaatttgc tgaacaatgc
    26941 cgttacgaag aagacgttgc acaactgcgc cagttactga aacagctgaa gcgttatttg
    27001 caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg tcatcgcatc
    27061 agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac attgattcca
    27121 ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag agcggttgtg gaaacgattt
    27181 atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg tgagagcaaa
    27241 acgctggccc tcacttcgcg cgaagcttaa tccagctggt gttgttgcat aaacggctca
    27301 ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac gtagcccgat
    27361 ggcgcggaaa ctttgaaacg aagtggatta ggtaatacag ctgccagtaa ggcagcttcc
    27421 gatcgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca
    27481 aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg ctttttgctc
    27541 cagaccgttt ctatccccag cgttaatccg gcttccagcc cttttcgaac ccagctacgc
    27601 ccatcccata aaaagagatt tttggcagtc tgctgagaaa tcgttgaagc accgcgaata
    27661 cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa tggaagcgac atcaaagccc
    27721 cagtgctcag gaaatgtctg atcttctgcg gcaatcaccg ccagtcccat ccacggcgag
    27781 atttgatcca tactgaccca gtcagaatgt gccacgtaac gaaaattgcc atgcagccag
    27841 gcgctgacct gtcgctcgac cattaccgct gagaaaggaa caggcgcaac gctaaacaac
    27901 gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcagaag aaaacggcga
    27961 acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc acgcgggaaa
    28021 ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata tatgccgggg
    28081 tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac ggcacatgct
    28141 ccgcgcccat ctcttccagc acttctgcgg tatcaatatc agtaccgatg gtcaaacgca
    28201 gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa ccaagcggtt
    28261 ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg gtgcattcgc
    28321 taccaagact ggcaaaattg cttaaattct tcgccgcgcc aaaccccccc ggcacaatca
    28381 acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg cgcgttattc
    28441 gtgccgcttc aatcagcaca ttacgcgttt ccgtcatcgc ttcgccagtt aaatggttga
    28501 taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa
    28561 tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc
    28621 tcagaattac gccaattttc ttcattgtga tcatcctttc gcaattgact gaaacacatt
    28681 aattttttta ataaaaatgc tacgcatcac acatttaact gattcatgta acaaatcatt
    28741 taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca
    28801 tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc
    28861 cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag cacttctacg
    28921 tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc ttcccaggca
    28981 ggaaggtccg gagactgaat ttgctgaccc agctgttgca gatggcgtaa ccccactgac
    29041 cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc
    29101 agattcgact ccagcacgct gacatagcct ggcatcatct tctcaaacac cgctaacccg
    29161 tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg aatatcgagc
    29221 aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc atcctgggta
    29281 tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt actcagcaca
    29341 tcatccattc cagcattgag atactcttgc ttgtctttca gcacgttagc ggttaaagcc
    29401 accagcggcg gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga gatatccagc
    29461 ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc cggtttaaac
    29521 atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa tttttccagc
    29581 acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagcagcac attcagcgcc
    29641 ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgccac cgacggtgcg
    29701 tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc tggtaaccgt aatatcgccg
    29761 cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc ggtggcaggt
    29821 ttaccgccat gactgtcttt cacctggtaa tacatggcga agattttatc cagctcatcc
    29881 tgcggaatgc cgataccaga atcttccact tcaaaatgca gcatatcgcc ttcatcgtaa
    29941 cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact gatgaggttc
    30001 cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta
    30061 ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca aggcggagag attttccaga
    30121 tcggcaagga agctggtgaa atcaaccggc tgattatcaa gctggacctt gcgccgttcc
    30181 atcttatcca tgtcgataat atcgttgaag atattcccca gcgtgacggc cgaaacatgg
    30241 atagttttga gatatttttc ctgctcggcg gtgagttcgg tatccagtag gatgcggctc
    30301 aggccgacga taccgttcag cggtgtacgc aattcgtgac tgatggtgga aataaacgtc
    30361 gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg
    30421 cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg cactttacga
    30481 atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata ggtcagtgac
    30541 acattatgac ggaaaacttt ttcatcggtt tcaatgactt tcgcagcggc ttccggtgag
    30601 taaacatcag caggtttcag atgaaccagt tgtttttcgc tttttccggt cagcagctcc
    30661 atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac caggtcgggt
    30721 gaagcatcaa ggaaggaacg taagaatgag gattgctgct cgagctgaat ttgtgtctct
    30781 tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc tgcttccgct
    30841 ttttcacgaa cggcgatttc ctgatttagc tgggcaatat tatcttttaa ctgaacgttg
    30901 aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga cagacgttgt
    30961 cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca cggcgtaatc
    31021 agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc atgcagcacc
    31081 atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc cagcaacatt
    31141 gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc cagcagacga
    31201 atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc aattctgaca
    31261 gttacgttat aaattgtgtg agaaatgcgg aattcccctc aacgacctac tccgttggag
    31321 gtatccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga tagcgatcca
    31381 gctcgaccat tcccgtccat cgattttcgc accacagagg agcaagcagc gtcggacgac
    31441 gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga atcatttctc
    31501 cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa acgccccgct tcccaggctt
    31561 tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg tccacgccgg
    31621 tttcaaccac tcgctccagc gtttgcaaac attcagcctg cccttcgcca ggcaggccga
    31681 caattaagtg ggaacacacc ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat
    31741 aacaggcaaa atcatggccg cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta
    31801 gccccagctc cagccacact tcgtagccct ggtccttata ttcgcaaagc agatccagca
    31861 ccgcatccgg cacgcagtcc gggcgagtac caacacacaa accgacaata ttggcctggc
    31921 tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg
    31981 cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt
    32041 gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa cgaggccaca ttacagaatg
    32101 tgcagccacc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg
    32161 tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac atattgacta
    32221 atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa gcctgccatt
    32281 tttagctcat ttcggcgatg acctggatca atcgtcccgg cctgccttta ccaactgcat
    32341 aatcaatcaa aattaccgaa atttcatgca taatcacata aatcactttt gcttatcttg
    32401 tgtcagattt ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgtcatc
    32461 accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta
    32521 acacgcctta atgacagtca ggaattggct gtttct
//
LOCUS       DAZUGK010000031        32141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZUGK010000031 DAZUGK010000000
VERSION     DAZUGK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.031"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1830)
                     /gene="ycaO"
                     /locus_tag="ACHY5W_001304"
     CDS             complement(70..1830)
                     /gene="ycaO"
                     /locus_tag="ACHY5W_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFS4446233.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1960..2652
                     /locus_tag="ACHY5W_001305"
     CDS             1960..2652
                     /locus_tag="ACHY5W_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4446234.1"
                     /translation="MKVFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2851..3939
                     /gene="serC"
                     /locus_tag="ACHY5W_001306"
     CDS             2851..3939
                     /gene="serC"
                     /locus_tag="ACHY5W_001306"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFS4446235.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4010..5293
                     /gene="aroA"
                     /locus_tag="ACHY5W_001307"
     CDS             4010..5293
                     /gene="aroA"
                     /locus_tag="ACHY5W_001307"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4446236.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKIIYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5462..6226
                     /gene="ycaL"
                     /locus_tag="ACHY5W_001308"
     CDS             5462..6226
                     /gene="ycaL"
                     /locus_tag="ACHY5W_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFS4446237.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6399..7082
                     /gene="cmk"
                     /locus_tag="ACHY5W_001309"
     CDS             6399..7082
                     /gene="cmk"
                     /locus_tag="ACHY5W_001309"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4446238.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7193..8866
                     /gene="rpsA"
                     /locus_tag="ACHY5W_001310"
     CDS             7193..8866
                     /gene="rpsA"
                     /locus_tag="ACHY5W_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4446239.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9026..9310
                     /gene="ihfB"
                     /locus_tag="ACHY5W_001311"
     CDS             9026..9310
                     /gene="ihfB"
                     /locus_tag="ACHY5W_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFS4446240.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9517..11781
                     /locus_tag="ACHY5W_001312"
     CDS             9517..11781
                     /locus_tag="ACHY5W_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFS4446241.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11818..13566
                     /gene="msbA"
                     /locus_tag="ACHY5W_001313"
     CDS             11818..13566
                     /gene="msbA"
                     /locus_tag="ACHY5W_001313"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFS4446242.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     LERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13563..14549
                     /gene="lpxK"
                     /locus_tag="ACHY5W_001314"
     CDS             13563..14549
                     /gene="lpxK"
                     /locus_tag="ACHY5W_001314"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFS4446243.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERVGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14586..15818
                     /gene="ycaQ"
                     /locus_tag="ACHY5W_001315"
     CDS             14586..15818
                     /gene="ycaQ"
                     /locus_tag="ACHY5W_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFS4446244.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMQAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRKTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDSVA"
     gene            15870..16052
                     /gene="ycaR"
                     /locus_tag="ACHY5W_001316"
     CDS             15870..16052
                     /gene="ycaR"
                     /locus_tag="ACHY5W_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFS4446245.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16049..16795
                     /gene="kdsB"
                     /locus_tag="ACHY5W_001317"
     CDS             16049..16795
                     /gene="kdsB"
                     /locus_tag="ACHY5W_001317"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS4446246.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16949..17842
                     /locus_tag="ACHY5W_001318"
     CDS             16949..17842
                     /locus_tag="ACHY5W_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFS4446247.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNSVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17819..18598)
                     /gene="elyC"
                     /locus_tag="ACHY5W_001319"
     CDS             complement(17819..18598)
                     /gene="elyC"
                     /locus_tag="ACHY5W_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFS4446248.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18734..19519
                     /gene="cmoM"
                     /locus_tag="ACHY5W_001320"
     CDS             18734..19519
                     /gene="cmoM"
                     /locus_tag="ACHY5W_001320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFS4446249.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19516..20838
                     /gene="mukF"
                     /locus_tag="ACHY5W_001321"
     CDS             19516..20838
                     /gene="mukF"
                     /locus_tag="ACHY5W_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFS4446250.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20819..21523
                     /gene="mukE"
                     /locus_tag="ACHY5W_001322"
     CDS             20819..21523
                     /gene="mukE"
                     /locus_tag="ACHY5W_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFS4446251.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21523..25983
                     /gene="mukB"
                     /locus_tag="ACHY5W_001323"
     CDS             21523..25983
                     /gene="mukB"
                     /locus_tag="ACHY5W_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFS4446252.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVEQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26244..28091
                     /gene="ldtD"
                     /locus_tag="ACHY5W_001324"
     CDS             26244..28091
                     /gene="ldtD"
                     /locus_tag="ACHY5W_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4446253.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28272..28820
                     /locus_tag="ACHY5W_001325"
     CDS             28272..28820
                     /locus_tag="ACHY5W_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFS4446254.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28847..29494
                     /gene="gloC"
                     /locus_tag="ACHY5W_001326"
     CDS             28847..29494
                     /gene="gloC"
                     /locus_tag="ACHY5W_001326"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFS4446255.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVND
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLKEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29545..30735)
                     /gene="aspC"
                     /locus_tag="ACHY5W_001327"
     CDS             complement(29545..30735)
                     /gene="aspC"
                     /locus_tag="ACHY5W_001327"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFS4446256.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30920..32008)
                     /gene="ompF"
                     /locus_tag="ACHY5W_001328"
     CDS             complement(30920..32008)
                     /gene="ompF"
                     /locus_tag="ACHY5W_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFS4446257.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGTDAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7804 a         7828 c         8669 g         7840 t
ORIGIN      
        1 cattaaaaaa acgccccgta atataactca gacgaatcat taagcctaca ttgcgtaggc
       61 tatttgattt tattttgccc agaatgctgc tttggcgcgc tgcagctttt cgtaggcctt
      121 caacagcgac tgatgtgcag caaacgcgtg cagatcgcta tctactggtt gcaagccata
      181 aaacgccgct tcgccgctca ttgccgcact agcggcttcc acggcatctg cgccgtacat
      241 gcgaacaaac gcattcagat attgcagcgg ctggcgatct tcttcctgcg ccagtaataa
      301 caacgtttgc aggcagcgat aatagttggc gcgttccggg ctgaataccg atgagttaaa
      361 ctccatcgtc cattcggtcc agaccagagc ctgttccaga tcgccaccag ccagcgccag
      421 catggctttt aattcaccga tacgcagggt gtaccaaccg ttatccgacc cggtcgccag
      481 acccaacagc tcacgcacgc gggtaaagtc atcaaaacct tcttcatcca gttgctcgat
      541 gaggttcagg taatcttctt tttcccactc gctgcctggt agcgaaagaa tcgtttcacg
      601 taaatggctg cccatactgt tattcgcgag ccacagatct tcagccggat aaatatcgga
      661 catgccaggc acgataatac ggcaagcata aacgcccaga tgctcgtaat cggcaatata
      721 aacttcttta tcttctttgt tgaagatagc catcagcgta gcaaactctt cttccgtggt
      781 gccggagaaa ttccagtcca caaacggata atcggcatcc tgcttgaaca ggtcccagga
      841 gattaaaccg ctggaatcga taaagtgcgt ttcgaggttg gtatgttcag cgacttcttc
      901 atcatcgaag gttggcggag taaacacatc caaatctttc aggccacgac cttgcagcag
      961 ctcggtcacg gtacgttcca gtgctacgcc aaaatcagga tgcgcaccga aagaggcaaa
     1021 gcaggtgccg ttagcaggat taaacagtac cacgcaaatc accggatact ggccgccaag
     1081 ggaaccatca tatgcgaaga tcgggaaacc ttctgcttcc agtgtttcga tcgcttcaac
     1141 caccgctggg taacgcgcca gcacgtctgc cgggatttcc ggcaagctga tgctttcagc
     1201 aataatgcgg tttttcacgt agcgttcgaa aacttcggac aacccctgaa cgcgtgcttc
     1261 gttgcgggta ttgccagcgg acataccgtt agaaacgtac aggttaccaa tgatattcat
     1321 cggaatataa acggtctgat tgtcggactg gcgcgtaaac ggcagaccgc aaataccacg
     1381 atcttcgtta ccggattgta ggtcaatcag catactgccg gtcaattcat tctccggatc
     1441 gtaaaatgcg cgcagacggt catcgagcag cccttctggc acatcgtcat tttcggtcag
     1501 tgggaaccat ttttcgttgg gataatgcac aaatggaccg ttggcgatgg tttcgcccag
     1561 ccagaaatca gcaaaaaagt agttggttga gagacgctcg aaatattcac cgagtgcaga
     1621 agccagcgcc gctttcttgg ttgcgccttt accgttggta aaacacagtg cgcactcttt
     1681 gtcgcgaata tgtacagacc agacgttagg cacgggattc agccaggagg cctcttcaat
     1741 ctgaaagccg aggtctgaaa gtttttgctg gaagcgagcg atggaatctt ccagtgcggc
     1801 atctttgccg gggataaatg tttgcgtcat gaaaatcact ttagtcgtac ggaaagcgcg
     1861 caataatacg ggttttatct caaaggcgct atcaccgccg ccatgccgat gaactgttga
     1921 ctatgctttt agcggataaa ccacgttaag agcataaaaa tgaaggtgtt cgatctccac
     1981 cgtatggcat ttgataaagt gccttttgat ttccttggcg aagttgcact acgtagtctg
     2041 tatacctttg tactggtttt tttgttcctc aaaatgaccg gaagacgcgg agtgcggcag
     2101 atgtcgctgt ttgaagtgtt aatcattctg acgctggggt cagcggcggg agatgtggcg
     2161 ttttatgatg atgtgccgat ggtcccggta cttatcgtct ttatcactct ggcgttgtta
     2221 taccgcctgg taatgtggtt gatggcacac agtgaaaaac tggaagatct tctggaaggt
     2281 aagccggttg tcattattga agatggcgag ctggcctggt cgaaactcaa taactccaac
     2341 atgacggaat ttgagttctt tatggagcta cgattgcgcg gtgtagagca gttggggcaa
     2401 gtacgtctgg cgattctcga aaccaacggg caaatcagtg tctatttctt tgaagatgac
     2461 aaggtgaaac cgggtttact tattttaccc agtgattgta ctcagcgtta caaagtggtg
     2521 ccggagtcgg cggactatgc ctgcatccgt tgtagtgaaa tcattcatat gaacgcgggg
     2581 gaaaaacaat tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag tcgggctaaa
     2641 cgggtgacct gacagcaaaa atatcggttt tttggtaata atccgagaga ttctgttgtg
     2701 tgacgcgtgc cgcatttttg ccctcaacgg ttttacccat tgcgatgcgt gtcactgaat
     2761 gataaaaccg atagccacag gaataacata ttacccgcgg tcgcaatcga ttgactgggg
     2821 gttaatggca acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt tagttctggt
     2881 ccggcaatgc taccggcaga ggtgcttaaa caggctcaac aggaactgcg tgactggaac
     2941 ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat tcaggttgca
     3001 gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta
     3061 ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat
     3121 aaaaccaccg cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg
     3181 aaaaaatact gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg
     3241 gttaagccaa tgcgtgaatg gcaactctct gataatgctg cttatatgca ttactgcccg
     3301 aatgaaacca tcgacggtat cgccatcgac gaaacgccag acttcggcaa agatgtggtg
     3361 gttgccgccg acttctcttc aaccattctt tcccgtccga ttgacgtcag ccgttatggt
     3421 gtaatttacg ctggcgcgca gaaaaatatc ggcccggcag gcctgacaat cgtcatcgtc
     3481 cgtgaagatt tgctgggcaa agcgaatgtc gcgtgtccgt cgattctgga ttattccatc
     3541 ctcaacgata atggctccat gtttaacacg ccgccgacat ttgcctggta tctgtctggt
     3601 ctggtcttta aatggctgaa agcgaacggc ggtgtagctg aaatggataa aatcaatcag
     3661 caaaaagcag aactgctata tggggtgatt gataacagcg atttctaccg caatgacgtg
     3721 gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt tggcggacag tgcgcttgac
     3781 aaattgttcc ttgaagagtc ttttgctgca ggccttcatg cactgaaagg tcaccgtgtg
     3841 gtcggcggaa tgcgcgcgtc tatttataat gccatgccgc tggaaggcgt taaagcgctg
     3901 acagacttca tggttgagtt cgaacgccgt cacggttaat gccgaaattt tgcttaatcc
     3961 ccacagccag cctgtggggt ttttatttct gttgtagaga gttgagttca tggaatccct
     4021 gacgttacaa cccatcgctc gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt
     4081 ttctaaccgc gctttattgc tggcggcatt agcacacggc aaaacagtat taaccaatct
     4141 gctggatagc gatgacgtgc gccatatgct gaatgcatta acagcgttag gggtaagcta
     4201 tacgctttca gccgatcgta cacgttgcga aattatcggt aacggcggtc cattacacgc
     4261 agaaggtgcc ctggagttgt tcctcggtaa cgccggaacg gcaatgcgtc cgctggcggc
     4321 agctctttgt ctgggtagca atgatattgt gctgaccggt gagccgcgta tgaaagagcg
     4381 cccaattggt catctggtgg atgcgctgcg cctgggcggg gcgaagatca tttacctgga
     4441 acaagaaaat tatccgccgt tgcgtttaca gggcggcttt actggcggca acgttgacgt
     4501 tgatggctcc gtttccagcc aattcctcac cgcactgtta atgactgcgc ctcttgcgcc
     4561 ggaagatacg gtgattcgta ttaaaggcga tctggtttct aaaccttata tcgacatcac
     4621 actcaatctg atgaagacgt ttggtgttga aattgaaaat cagcactatc aacaatttgt
     4681 cgtaaaaggc gggcagtctt atcagtctcc gggtacttat ttggtcgaag gcgatgcatc
     4741 ttcggcttct tactttctgg cagcagcagc aatcaaaggc ggcactgtaa aagtgaccgg
     4801 tattggacgt aacagtatgc agggtgatat tcgctttgct gatgtgctgg aaaaaatggg
     4861 cgcgaccatt tgctggggcg atgattatat ttcctgcacg cgtggtgaac tgaacgctat
     4921 tgatatggat atgaaccata ttcctgatgc ggcgatgacc attgccacgg cggcgttatt
     4981 tgcaaaaggc accaccacgc tgcgcaatat ctataactgg cgtgttaaag aaaccgatcg
     5041 cctgtttgcg atggcaacag aactgcgtaa agtcggtgcg gaagtagaag aggggcacga
     5101 ttacattcgt atcactccac cggaaaaact gaactttgcc gagatcgcga catacaatga
     5161 tcaccggatg gcgatgtgtt tctcgctggt ggcgttgtca gatacaccag tgacgattct
     5221 tgatcccaaa tgtacggcca aaacatttcc ggattatttc gagcagctgg cgcggattag
     5281 ccaggcagcc tgaatgaata acgtccaata aatagccaaa actttcttta tcaaaacgtc
     5341 ggcacattgc cggcgttttt tttcggacct tgtgagtcat tttgattaat ggtagcgtca
     5401 gttgtcaatg taagttgttg atacataata tttatatatg attaatcaac ggatgattca
     5461 catgaagaat actaaattac tgctggcgat tgtgacctct gcagcattac tgacggggtg
     5521 tcaaaatacc cacggtattg ataccaatat ggctatcagc tccggtttaa atgcctataa
     5581 agcagcaaca ttaagcgatg ccgatgcaaa agcgattgcc aatcagggct gtgccgaaat
     5641 ggacagcggc aatcaagtcg caagtaaatc cagcaagtac ggtaaacgtc tggcaaaaat
     5701 cgccaaagca ttgggtaaca atattaatgg cacgccggtc aactataagg tttatatgac
     5761 cagcgacgtc aacgcatggg cgatggcgaa cggctgtgtt cgtgtttaca gtggcctgat
     5821 ggacatgatg aacgataacg aaattgaagg cgttctgggc catgaactgg gccacgtcgc
     5881 gttgggtcac tcgctggctg aaatgaaagc ttcttatgcg atcgttgccg cacgcgatgc
     5941 catttcagct accagcggtg tggcttccca gctttcccgc tcacaattgg gtgatatcgc
     6001 agaaggcgct atcaatgcga aatactcccg cgataaagag tccgaagcag atgatttctc
     6061 ctttgatctg ttgaagaaac gtggcatcag cacccagggg ctggttggca gctttgaaaa
     6121 actggctagc ctggatggcg gtcgcaccca gtccatgttt gactctcacc caccatcaac
     6181 agagcgtgcg caacacatcc gtgatcgtat cgcctctggt aagtaaatca ttgtcatctt
     6241 tcgggctggt cttctgccag cccgctataa ttgcgcaata aatccccatc tgaatacaga
     6301 caaaactggt ttttgcacac aacgttaacg atttgtggcg tcggcgcgta taatgcgcgc
     6361 ggttatgtta acggtacgcc tgttttaagg agataaagat gacggcaatt gccccggtta
     6421 ttaccattga tggcccaagc ggtgcaggga aaggcacctt gtgtaaggct atggcggaag
     6481 cgttgcaatg gcatctgctg gattcgggtg caatttatcg cgtactggca ttggcggcat
     6541 tacatcacca tgttgatgtt gcgtcggaag acgcgctggt gccgctggca tcccatctgg
     6601 atgtacgttt tgtgtcaacc aatggcaatc tggaagtgat cctcgaaggg gaagatgtca
     6661 gcggcgaaat tcgtactcag gaagtggcga atgcagcttc acaagtcgcg gcattcccac
     6721 gcgttcgtga agcattattg cgtcgccaac gcgcgtttcg cgaattacca ggcctgattg
     6781 ccgatggccg cgacatggga acggtggtat tccctgatgc gccagtgaaa attttccttg
     6841 acgcctcctc ggaagaacgt gcgcatcgcc gcatgctaca gttgcaggag aagggcttta
     6901 gtgttaactt tgagcgcctt ttggccgaga tcaaagaacg cgacgaccgc gatcgtaacc
     6961 gagcggtagc gccactggtt ccggcagccg atgctttagt gttggattcc accaccttaa
     7021 gcattgagca agtgattgaa aaagcgctac aatacgcgcg ccagaaattg gctctcgcat
     7081 aagcgaccga atttgcagta cccccgttgc aatggaatga cagcgggtat gttaaacaac
     7141 cccatccggc atggagccag gtggacgtta aatataaacc tgaagattaa acatgactga
     7201 atcttttgct caactctttg aagagtcctt aaaagaaatc gaaacccgcc cgggttctat
     7261 cgttcgtggc gttgttgttg ctatcgacaa agacgtagta ctggttgacg ctggtctgaa
     7321 atctgagtcc gccatcccgg ctgagcagtt caaaaacgcc cagggcgagc tggaaatcca
     7381 ggtaggtgac gaagttgacg ttgctctgga cgcagtagaa gacggcttcg gtgaaactct
     7441 gctgtcccgt gagaaagcta aacgtcacga agcctggatc acgctggaaa aagcttacga
     7501 agatgctgaa actgttaccg gtgttatcaa cggcaaagtt aagggcggct tcactgttga
     7561 gctgaacggt attcgtgcgt tcctgccagg ttctctggta gacgttcgtc cggtgcgtga
     7621 cactctgcac ctggaaggca aagagcttga attcaaagta atcaagctgg atcagaagcg
     7681 caacaacgtt gttgtttctc gtcgtgccgt tatcgaatcc gaaaacagcg cagagcgcga
     7741 tcagctgctg gaaaacctgc aggaaggcat ggaagttaaa ggtatcgtta agaacctcac
     7801 tgactacggt gcattcgttg atctgggcgg cgttgacggc ctgctgcaca tcactgacat
     7861 ggcctggaaa cgcgttaagc atccgagcga aatcgtcaac gtgggcgacg aaatcactgt
     7921 taaagtgctg aagttcgacc gcgaacgtac ccgtgtatcc ctgggcctga aacagctggg
     7981 cgaagatccg tgggtagcta tcgctaaacg ttatccggaa ggtaccaaac tgactggtcg
     8041 cgtgaccaac ctgaccgact acggctgctt cgttgaaatc gaagaaggcg ttgaaggcct
     8101 ggtacacgtt tccgaaatgg attggaccaa caaaaacatc cacccgtcca aagttgttaa
     8161 cgttggcgat gtagtggaag ttatggttct ggatatcgac gaagaacgtc gtcgtatctc
     8221 cctgggtctg aaacagtgca aagctaaccc gtggcagcag ttcgcggaaa cccacaacaa
     8281 gggcgaccgt gttgaaggta aaatcaagtc tatcactgac ttcggtatct tcatcggcct
     8341 ggacggcggc atcgacggcc tggttcacct gtctgacatc tcctggaacg ttgcaggcga
     8401 agaagcagtt cgtgaataca aaaaaggcga cgaaatcgct gcagttgttc tgcaggttga
     8461 cgcagaacgt gaacgtatct ccctgggcgt taaacagctc gcagaagatc cgttcaacaa
     8521 ctgggttgct ctgaacaaga aaggcgctat cgtaaccggt aaagtgactg cagttgacgc
     8581 taaaggcgca accgtagaac tggctgatgg cgttgaaggt tacctgcgtg cttctgaagc
     8641 atcccgtgac cgcgttgaag acgctaccct ggttctgagc gttggcgacg aagttgaagc
     8701 taaattcacc ggcgttgatc gtaaaaaccg cgcaatcagc ctgtctgtac gtgcgaaaga
     8761 cgaagctgac gagaaagatg caatcgcaac tgttaacaaa caggaagatg caaacttctc
     8821 caacaacgca atggctgaag ctttcaaagc agctaaaggc gagtaattct ctgactcttc
     8881 gggattttta ttccgaagtt tgttgagttt acttgacaga ttgcaggttt cgtcctgtaa
     8941 tcaagcacta agggcggcta cggccgccct taatcaatgc agcaacagca gccgcttaat
     9001 ttgcctttaa ggaaccggag gaatcatgac caagtcagaa ttgatagaaa gacttgccac
     9061 ccagcaatcg cacattcccg ccaagacggt tgaagatgca gtaaaagaga tgctggagca
     9121 tatggcctcg actcttgcgc agggcgagcg tattgaaatc cgcggtttcg gcagtttctc
     9181 tttgcactac cgcgcaccac gtaccggacg taatccgaag actggcgata aagtagaact
     9241 ggaaggaaaa tacgttcctc actttaaacc tggtaaagaa ctgcgcgatc gcgccaatat
     9301 ttacggttaa gtttttactc aaacttgaac gcgagaaaag cacctgtcgg gtgctttttt
     9361 catttctcta atcaggagct ggaagctgcc tcgcagagtt ttgagcagtt ttcacccttt
     9421 cgttaaattc ttctgaatgt gcctcgggga acgcaaaatt cccaccagac aaccgctcaa
     9481 caaagttgca cactttccat aaacagggag gggtgcatga aaataacgac agtcggcgta
     9541 tgcataattt gcggaatttt tccgttgctg attttgcccc aattgcctgg gacattaacc
     9601 cttgcgtttc tgactctctt cgcctgcgta ctggcattta tccctgttaa aaccgtccgt
     9661 tatatcgcgc tgacgttgct gtttttcgtt tggggcatat tatcagcaaa gcaaattttg
     9721 tgggcaggag aaaccttaac tggcgcgacg caggatgcaa ttgttgagat cactgctact
     9781 gacggcatga ccactcatta tggtcaaatt acttatctac aaggtcgacg tatattccct
     9841 gcgccaggcc ttgtgctgta tggcgaatat cttccgcaag cggtttgtgc cggacaactg
     9901 tggtcaatga aactcaaagt tcgtgccgtt catggtcaac ttaatgatgg cggctttgat
     9961 agccagcgtt atgccattgc ccagcatcag ccgctcaccg gccgctttct gcaggcaagt
    10021 gttattgaac cgaattgtag cctgcgtgca cagtatctgg cgtcactaca aacaacgctg
    10081 caaccctatc cgtggaatgc ggttattctt ggtttaggta tgggggaacg gttatccgtc
    10141 cctaaagaaa tcaaaaatat catgcgtgat actggaacgg cgcatttaat ggcgatatcg
    10201 ggattgcaca tcgcttttgc ggcgttgctg gctgccggac tcattcgcag tggacaaatt
    10261 tttctgcctg ggcgctggat ccactggcaa atgccattaa ttggcggaat ctgctgtgct
    10321 gctttttatg cctggctgac gggaatgcaa cctcctgcat tgcgtaccgt tgtggcgctt
    10381 gctacgtggg gaatgcttaa gttaagtggg cgacagtgga gtggctggga tgtatggata
    10441 tgttgtctgg cggcaatttt gctgatggat cctgttgcca ttctctcgca aagtttatgg
    10501 ctctctgccg ctgcggtcgc ggcattgata ttttggtatc agtggtttcc ctgtcctgag
    10561 tggcaactgc cgccggtatt gcgtgcagtt gtttccctca tccatctgca actgggaatc
    10621 acacttctgc ttatgcccgt gcaaatcgtc atatttcatg gcattagcct gacctcgttt
    10681 attgcaaatc tattagctat tcccttggtg acatttatca cggttccgtt gatcctcgcc
    10741 gcgatggttg tgcatttaag cgggccgtta atcctggagc aagggttatg gtttcttgcc
    10801 gaccggtctt tggctttact tttctggggg ttaaagagtt tgccagaagg gtggatcaac
    10861 attgctgaac gttggcaatg gctatcattt tccccatggt tcttactggt ggtatggcga
    10921 ttaaacgcct ggcgaacgtt gccagcaatg tgtgtggctg taggcttgct gatgtgctgg
    10981 ccgctgtggc aaaaacctcg acctgacgag tggcaagtgt acatgcttga tgtcgggcaa
    11041 gggctggcaa tggtgatagc cagaaacggc aaagcgattc tctatgacac gggactggcc
    11101 tggcctgaag gggatagtgg gcaacaactg attatcccct ggctccactg gcataatctt
    11161 gaaccggaag gcgttattct gagtcatgaa catctggatc accggggagg gctggactca
    11221 atattgcaca catggccgat gttatggatc agaagtccgt taaactggga acatcatcag
    11281 ccctgtgtgc gtggcgaagc atggcaatgg caaggattgc gtttcagcgt gcactggcct
    11341 ttacaagcta gcaacgataa aggaaataac cattcctgtg tggttaaggt tgatgacggg
    11401 acgaatagca ttcttctaac cggtgatatt gaagtccccg ctgaacaaaa gatgctaagc
    11461 cgttactggc agcaagtgca ggcaacattg cttcaggtac ctcaccatgg cagtaatacc
    11521 tcatcatcgt tgccattaat tcagcgagtg aatggaaaag tggcactcgc atcggcatcg
    11581 cgctataacg catggcgact gccctctaac aaagttaagc atcgctatca acaacagggt
    11641 tatacgtggc ttgatactcc tcatcagggg caagtaacgg tcgatttttc agcgcaaggc
    11701 tggcggatta gcagcctcag ggagcaaatt ttacctcgtt ggtatcatca gtggtttggc
    11761 gtgccagtgg ataacgggta gaatatgcgg ctatttcaac aaatgctggt tttttgaatg
    11821 cataacgaca aagatctctc tacgtggcag acattccgcc gactgtggcc aaccattgcg
    11881 ccttttaaag cgggtctgat cgtggcgggc gtagcgttaa tcctcaacgc agccagcgat
    11941 accttcatgt tatcgctcct taagccactt cttgatgatg gctttggtaa aacagatcgc
    12001 tccgtgctgg tgtggatgcc gctggtggtg atcgggctga tgattttgcg tggtatcacc
    12061 agctatgtct ccagctactg tatctcctgg gtatcaggaa aggtggtaat gaccatgcgt
    12121 cgccgcctgt ttggtcacat gatgggaatg ccggtttcat tctttgacaa acagtcaacg
    12181 ggtacgctgt tgtcacgtat cacctacgat tccgaacagg ttgcttcttc ctcttccggc
    12241 gcactgatta ctgttgtgcg tgaaggtgcg tcgatcatcg gcctgttcat catgatgttc
    12301 tattacagtt ggcaattgtc gatcattttg attgtgctgg caccgattgt ttcgattgcg
    12361 attcgcgtag tatcgaagcg ttttcgcaac atcagtaaaa acatgcagaa caccatgggg
    12421 caggtgacca ccagcgctga acaaatgctg aaaggccata aagaagtatt gattttcggt
    12481 ggtcaggaag tggaaacgaa acgcttcgat aaagtcagca accgaatgcg tcttcagggg
    12541 atgaaaatgg tttcagcctc ttccatctct gatccgatca ttcagctgat cgcctctttg
    12601 gcgctggcgt ttgttctgta tgcggcgagc ttcccaagtg tcatggatag cctgactgcc
    12661 ggtacgatta ccgttgtttt ctcttcaatg attgcactga tgcgtccgct gaaatcgctg
    12721 accaacgtta acgcccagtt ccagcgcggt atggcggctt gtcagacgct gtttaccatt
    12781 ctggacagtg agcaggagaa agacgaaggt aagcgcgtgc tcgagcgtgc gactggcgac
    12841 gtggaattcc gcaacgtcac ctttacttat ccgggacgtg acgtacccgc attgcgtaac
    12901 atcaacctga aaattccggc agggaagacg gttgctctgg ttggacgctc tggttcaggt
    12961 aaatcaacca tcgccagcct gatcacgcgt ttttacgata ttgatgaagg cgaaatcctg
    13021 atggatggtc acgatctgcg cgagtatact ctggcgtcgt tacgtaacca ggttgctctg
    13081 gtgtcgcaga atgtccatct gtttaacgat acggttgcta acaacattgc ttacgcacgg
    13141 actgaacagt acagccgtga gcaaattgaa gaagcggcgc gtatggccta cgccatggac
    13201 ttcatcaata agatggataa cggtctcgat acagtgattg gtgaaaacgg cgtgctgctc
    13261 tctggcggtc agcgtcagcg tattgctatc gctcgagcct tgttgcgtga tagcccgatt
    13321 ctgattctgg atgaagctac ctcggctctg gataccgaat ccgaacgtgc gattcaggcg
    13381 gcactggatg agttgcagaa aaaccgtacc tctctggtga ttgcccaccg cttgtctacc
    13441 attgaaaagg cagacgaaat cgtggtcgtc gaggatggtg tcattgtgga acgcggtacg
    13501 cataacgatt tgcttgagca ccgcggcgtt tacgcgcaac ttcacaaaat gcagtttggc
    13561 caatgatcga aaaaatctgg tctggtgaat cccctttgtg gcggctattg ctgccactct
    13621 cctggttgta tggcctagtg agtggcgcga tccgtctttg ctataaacta aaactgaagc
    13681 gcgcctggcg tgcccccgta ccggttgtcg tggttggtaa tctcaccgca ggcggcaacg
    13741 gaaaaacccc ggtcgttgtc tggctggtgg aacagttgca acagcgcggt attcgcgtgg
    13801 gggtcgtgtc ccggggatat ggtggtaagg ctgaatctta tccgctgtta ttgtcggcag
    13861 ataccaccac agcacaggcg ggtgatgaac ctgtgttgat ttatcaacgc actggcgcgc
    13921 ccgttgcggt ttctcccgtg cgttctgatg cggtaaaagc cattctggcg caacaccctg
    13981 atgtgcagat catcgtaacc gacgacggtt tacagcatta ccgtctggcg cgtgatgtgg
    14041 aaattgtcgt tgttgatggt gtgcgtcgct ttggcaatgg ctggtggttg ccggcggggc
    14101 caatgcgtga gcgagtgggg cgcttaaagt cagttgatgc ggtaatcgtc aacggcggtg
    14161 tcccccgcag cggtgaaatc cccatgcatc tgctgccggg tcaggcggtg aatttacgta
    14221 ccggtacgcg ttgtgacgtt gctcagcttg aacatgtggt ggcgatggca gggattgggc
    14281 atccgccgcg cttttttgcc acgctgaaga tgtgcggcgt acaaccggaa aaatgtgtac
    14341 cgctggccga tcatcagtct ttgaaccatg cggatgtcag cgcgttggta agcaccgggc
    14401 aaacgctggt aatgactgaa aaagatgcgg tgaaatgccg ggcctttgca gaagaaaatt
    14461 ggtggtattt gcccgttgac gcacagcttt caggtgatga accagcgaaa ctgcttgcgc
    14521 aactaacctc gctggcttct ggcaactagt tacgccgcgg cagcgttcga ttgatggagt
    14581 catgaatgtc gctgccgcac ctctcccttg ctgatgcgcg taatcttcac cttgccgcgc
    14641 aaggcctgtt aaacaaaccc cgccgtcgag cgtcgttgga ggatattccg gcaacgatct
    14701 cccgcatgtc cttgctgcaa atcgatacca tcaatattgt tgcccgtagc ccatatctgg
    14761 tgcttttcag tcgtctggga aattatcctg cccagtggct agatgagtct ctggcgcgtg
    14821 gcgaattaat ggaatactgg gcgcatgaag cctgctttat gccacgcagc gactttcgtc
    14881 ttattcgcca ccgcatgcag gcacctgaaa aaatgggctg gaaatacaaa gacgcctgga
    14941 tgcaggaaca tgaggcggaa attgcacagt taattcagca tattcatgat aaggggccgg
    15001 tacgttcagc cgattttgag catcctcgta aaggtgcaag cggctggtgg gaatggaagc
    15061 cgcataaacg gcatctggaa ggtttattta ctgccggaaa ggtgatggtg attgaacggc
    15121 gcaacttcca gcgcgtttat gatttaaccc accgtgtcat gcctgactgg gatgatgagc
    15181 gcgatctcgt ttcgcaaaca gaagcagaaa tcatcatgct ggataacagt gcgcgtagcc
    15241 tgggaatatt ccgcgaacag tggctggcag attactatcg gctgaaacgt ccggcactgg
    15301 cggcgtggcg tgaagcgagg gctgaacagc agcaaatcat tgctgtgcat gttgaaaaat
    15361 tgggcaatct ttggctgcat gctgatttgc tgccgctact cgagcgagcg ctggccggaa
    15421 agctcactgc aacgcacagc gcggtacttt cgccttttga tcctgttgtc tgggatcgca
    15481 aacgcgcaga gcagcttttt gattttagct accggctgga gtgctatacc ccagcgccga
    15541 aacgtcagta tggctatttt gttctgccat tattacatcg tgggcaatta gttgggcgaa
    15601 tggatgccaa aatgcatcgc aagacaggca tccttgaagt tatctctctg tggttacagg
    15661 aaggcattaa accaacgaca atgctgcaaa aagggttacg tcaggcgatt actgatttcg
    15721 cgagctggca gcaggcaacg cgggtgacat taggacgctg cccgcaaggt ctctttactg
    15781 attgccgcac tggctgggaa atagactccg tcgcataaat gattatgata agatttatgc
    15841 attcataacc cgatccatct ggaggaacta tggatcatcg tctgcttgaa atcattgcct
    15901 gcccggtttg caacggaaaa ctttggtata accaggaaaa acaagagctt atttgcaaac
    15961 tggacaacct ggctttcccg ctgcgcgatg gcattccagt gttgctggaa acggaagccc
    16021 gcgtgctgac tgctgatgag agtaaatcat gagttttgtg gtcattattc ccgcgcgcta
    16081 cgcgtccacg cgtctgcccg gtaaaccatt ggttgatatt aacggcaaac ccatgattgt
    16141 tcatgttctt gaacgcgcgc gcgaatcagg tgccgagcgc atcatcgtgg caaccgatca
    16201 tgaggatgtt gcccgcgccg ttgaagccgc tggcggtgaa gtatgtatga cgcgcgccga
    16261 tcatcagtca ggaacagaac gtctggcgga agttgtcgaa aaatgcgcat tcagcgacga
    16321 cacggtgatc gttaatgtgc agggtgatga accgatgatc cctgcgacaa tcattcgtca
    16381 ggttgctgat aacctcgctc agcgtcaggt gggtatggcg actctggcgg tgccaatcca
    16441 caatgcggaa gaagcgttta acccgaatgc ggtgaaagtg gttctcgacg ctgaagggta
    16501 tgcactgtac ttctctcgcg ccaccattcc ttgggatcgt gatcgttttg cagaaggcct
    16561 tgaaaccgtt ggcgataact tcctgcgtca tcttggtatt tatggctacc gtgcaggctt
    16621 tatccgtcgt tacgtcaact ggcagccaag tccgttagaa cacatcgaaa tgttagagca
    16681 gcttcgtgtt ctgtggtacg gcgaaaaaat ccatgttgct gttgctcagg aagttcctgg
    16741 cacaggtgtg gatacccctg aagatcttga gcgcgttcgc gctgaaatgc gctaatttca
    16801 cttcacgaca cttcagccaa ttttgggagg agtgtcgtac cgttacgatt ttcctcaatt
    16861 tttcttttca acaattgatc tcattcaggt gacatctttt atattggcgc tcattatgaa
    16921 agcagtagct tttatgaggg taatctgaat ggaacagctg cgtgccgaat taagccattt
    16981 actgggcgaa aaactcagtc gtattgagtg cgtcaatgaa aaagcggata cggcgttgtg
    17041 ggctttgtat gacagccagg gaaacccaat gccgttaatg gcaagaagct ttagtacgcc
    17101 cggaaaagcc cgacaactgg catggaaaac caccatgctg gcaagaagtg ggactgtccg
    17161 tatgccgact atttatggtg tgatgacgca tgaagaacac cccggccccg atgtcctgct
    17221 actggagcgg atgcgtggtg tttcggtgga ggcaccagcc cgaacaccag aacgctggga
    17281 acaactcaaa gaccaaatcg ttgaagcctt actggcctgg caccgtcagg acagtcgcgg
    17341 ttgcgtcggc gcggtcgaca atactcagga aaatttctgg ccctcatggt accggcaaca
    17401 tgttgaagtg ctatggacca cgctcaatca gttcaataac accggtctga cgatgcagga
    17461 taagcggatc ctgtttcgca ctcgcgaatg tctcccggca ttatttgaag gctttaacga
    17521 caatagtgtg ttgattcacg gtaacttctg tttacgcagc atgctgaaag attcgcgcag
    17581 cgatcagtta ctggcgatgg tcgggccggg actaatgctt tgggcaccgc gagaatacga
    17641 actgttccga ctaatggata attctctggc ggaagatttg ctctggagtt acctgcaacg
    17701 cgcgccagtg gcggagtcgt tcatctggcg gcgttggttg tatgtgttat gggatgaagt
    17761 tgcgcaactg gttaataccg gacgatttag tcggcgcaac ttcgatctgg catcaaaatc
    17821 actcttgccg tggctcgcct gacgaacctt ttagccactg ccagatacgg ccaagcgttt
    17881 catagccaac acgatcgcta tgcatcaacc agaccggaga agggattgcc cgttcccacg
    17941 ggttaagcgg cgagtcgatg gccagctgat tcgccggggc tggtagcgga tttaaccctt
    18001 cctgctgaaa aaagatcatc gcccgcggca gatgtgacgc ggaggtcacc agcaggaaag
    18061 gggcgtcacc aatcgcctgt ttcactgccg cagcttcttc ttcggtatct tttggcaaat
    18121 ccagggtgat aatttgctcg cgcggcacgc ccagcgattg cgcaactctg gcacctactt
    18181 ccgctgtact caccgtattg gtttttgcta cgcctcccgt gaagatcagt tttgatcccg
    18241 gattttcgcg ccataagcga atgccttcat tcaggcgtgg cagactgtta ttgattaaat
    18301 tagagctcgg tgcccactgc gggttccagg tataaccacc tccgagcacc acgatatagt
    18361 ccactttttg cgaattattc catgtcggat aggtgctttc gatgggacgc agcaggcggt
    18421 cggctaccgg ttgcaaactc aataacaaga gtgccagcca cccaatgcta ataaaaatct
    18481 tcccggtttt ttgaaaacgg ctaaaccaca gcagtgccag gccagcgcca ataattaaca
    18541 acatcagcgg aagtggcagc aacatattgc caatcacttt tttcagtgta aaaagcatcc
    18601 tttctggttc cttttttaac catatagcaa gggatcgcca gcgatattac accagacggg
    18661 ttcattctcg ccgtggctgt gacaaaatag cgtctttatc agaaaacgtc ttagccaacc
    18721 ggtggagaaa gcaatgcagg atcgcaattt tgatgatatt gcggaaaagt tttcccgtaa
    18781 catttacggc accaccaaag ggcagcttcg acaggctatt ctgtggcagg atctcgatcg
    18841 cgtgctggcg gaaatgggcc cgcaaaaact gcgtgtgctg gatgcgggcg gtggagaagg
    18901 gcagactgca atcaaaatgg ccgagcgtgg gcatcaggtt attttatgcg atctttctgc
    18961 gcagatgatc gaccgcgcaa aacaggcggc agaagcaaaa ggtgtgagcg acaacatgca
    19021 atttatacat tgcgccgctc aggatgttgc ttcgcatttg gaaacgcccg ttgatctgat
    19081 attgttccat gcggtgctcg agtgggtggc tgatccccgc agcgtattgc agaccttgtg
    19141 gtcagtgttg cgtccaggcg gcgtgttgtc gttaatgttc tacaatgcgc atggtttgtt
    19201 gatgcataac atggttgccg ggaattttga ttacgtgcag gcgggaatgc cgaaaaagaa
    19261 aaaacggacg ctttcgccag attatccacg cgacccgacg caggtttatc tgtggctgga
    19321 agaagctggt tggcaaatta tgggtaagac aggcgttcgc gtgtttcatg attatctgcg
    19381 cgagaagcac cagcagcgcg actgctatga agcattactt gaattagaaa cgcgttattg
    19441 ccgtcaggaa ccgtatatta ccctggggcg ttatattcat gtcaccgcgc gcaaaccgca
    19501 gagcaaggat aaagtatgag tgaattttcc cagacagtcc ccgaactggt tgcctgggcc
    19561 agaaaaaatg acttctccat ttcgctgccg gtagaccgac tctcttttct gctggcggtt
    19621 gccacgctga acggcgagcg tctggatggt gagatgagtg aaggcgagct ggtggatgca
    19681 ttccgccatg tgagtgatgc gtttgagcaa accagcgaaa ccatcggcgt gcgcgccaac
    19741 aacgcgatca acgacatggt gcgtcaacgt ctgctgaacc gctttaccag cgagcaggca
    19801 gaagggaacg caatttaccg tctgacgccg ctcggcatcg gcattactga ctattacatc
    19861 cgtcagcgcg agttttctac gctgcgcctt tctatgcagt tgtcgattgt ggcgggtgag
    19921 ctcaaacgcg cagcagatgc cgccgaagag ggcggtgatg aatttcactg gcaccgtaat
    19981 gtctatgcgc cactgaaata ttcggtagca gaaattttcg acagtatcga cctgacgcaa
    20041 cgtctgatgg acgaacagca gcagcaggtg aaggacgata tcgcccagtt gctgaacaaa
    20101 gactggcggg cggcgatttc cagctgtgaa ttgttgcttt cggaaacttc cggaacgctg
    20161 cgtgaattgc aggatacgct ggaagcggca ggcgacaaat tgcaggctaa tctgttgcgc
    20221 attcaggatg cgacgatgac ccatgacgat ctgcatttcg tcgatcgtct ggtgttcgat
    20281 ctgcagagca aactcgatcg tattatcagt tggggccagc aatccatcga cttgtggatt
    20341 ggctacgacc gccacgtaca caaatttatt cgtaccgcga tcgatatgga taaaaaccgc
    20401 gtctttgctc agcggttacg tcagtcggta caaacctatt ttgatgagcc gtgggcgcta
    20461 acttatgcca atgccgatcg tctgctggat atgcgtgacg aagagatggc actgcgcgat
    20521 gaagaagtga ctggggaact tcctgaggat ctggaatacg aagagtttaa cgagatccgc
    20581 gaacagctgg cggcgatcat cgaagaacaa cttgctgtgt acaaaaccag acaagtgccg
    20641 ctggatcttg gtctggtggt acgcgaatat ctgtcacagt atccgcgtgc acgtcacttt
    20701 gacgttgcgc gcattgttat tgatcaggcg gtacgtcttg gcgtagcgca agcagatttc
    20761 accggactgc cagcgaaatg gcagccgatt aatgattacg gagccaaggt acaggcgcat
    20821 gtcatcgaca aatattgaac aagtgatgcc ggttaagctg gcgcaggcgc tggcgaatcc
    20881 gttatttccg gcgctggaca gcgccttacg ttcaggccgc catattggcc tcgacgaact
    20941 ggataatcat gcattcctga tggattttca ggaatatctg gaagagtttt acgcgcgtta
    21001 taacgttgag cttattcgtg caccggaagg gttcttctat ttacgtccac gttccaccac
    21061 gctgatccct cgttccgtct tgtcggaact ggatatgatg gtcgggaaaa tcctctgtta
    21121 tctctatctc agcccggaac ggctggcgaa tgaggggatt ttcacccagc aggaactgta
    21181 cgacgaactg ctcaccctgg ccgatgaagc gaaactgctg aaactggtga acaaccgttc
    21241 aaccggttca gacgttgacc gtcagaagtt gcaggaaaaa gtacgttctt cgctcaaccg
    21301 tctgcgtcgt ttaggcatgg tgtggtttat gggccacgac agcagcaagt tccgcattac
    21361 cgaatcggtg ttccgcttcg gagccgatgt gcgtgctggc gacgatcccc gtgaagcaca
    21421 gcgtcgcctg attcgtgatg gcgaagcaat gccgattgaa aatcatctgc aactcaacga
    21481 tgaaaccgaa gagaatcagc cagatagcgg agaggaagaa taatgattga acgcgggaaa
    21541 tttcgctcac tgacgctgat taactggaac ggcttttttg cccgaacttt tgaccttgac
    21601 gagctggtca cgacgctttc tggcggtaac ggggcgggta aatccaccac catggcggcg
    21661 ttcgttacgg cgctgatccc cgacctgacc ctgctgcatt tccgtaacac tacggaagcc
    21721 ggggccacca gcggttcgcg cgataaaggt ctgcacggta agctgaaagc gggtgtctgt
    21781 tattcgatgc tcgacaccat taactcgcgc caccagcgcg tggtggtcgg tgtgcgtctg
    21841 caacaggttg ccggacgcga tcgtaaagtg gatatcaaac catttgccat tcagggactg
    21901 ccgatgtcgg tgcagccgac acagctggtg accgaaactc tgaacgaacg ccaggcgcgc
    21961 gtgctgccgc ttaacgagct gaaagacaag ctcgaggcga tggaaggcgt gcagtttaaa
    22021 cagttcaact ccattaccga ttaccactcg ctgatgttcg atctgggcat catcgcgcgt
    22081 cgtctgcgct ccgcatctga ccgtagcaaa ttctatcgtc tgatcgaagc ttcgctgtat
    22141 ggcgggatct ccagtgccat tacccgttct ctgcgcgact acctgttgcc ggaaaacagc
    22201 ggcgtgcgta aagcgttcca ggacatggaa gcggcgctgc gtgaaaaccg catgacgctg
    22261 gaagcgattc gtgtcaccca gtcggaccgt gacctgttta agcatctgat cagcgaagct
    22321 accaactacg tggcggcgga ctacatgcgt cacgccaacg agcgccgtgt ccatctcgac
    22381 aaagccctgg agtttcgtcg cgagctacat acttcgcgtc agcaactggc ggctgagcag
    22441 tacaagcacg tcgatatggc gcgtgagctg gcagagcaca acggtgccga aggcgatctg
    22501 gaagcggatt atcaggcggc cagcgatcac ctgaacctgg tgcaaaccgc actgcgtcag
    22561 caggagaaga tcgaacgcta cgaagcggat ctcgatgagc tgcagatccg tctggaagag
    22621 caaaacgaag tggtggcaga agccatcgaa cgccaggaag agaacgaggc tcgtgcggaa
    22681 gctgccgaac tggaagtgga cgagctgaaa agccagcttg ctgactacca gcaggcgctg
    22741 gacgtccagc aaacgcgcgc gatccagtat aaccaggcga ttgctgcgct taatcgtgcc
    22801 aaagaactgt gccatctgcc ggacttaacc gccgacagcg ccgccgaatg gctggaaacc
    22861 ttccaggcga aagagctgga agcgactgaa aaaatgctct ctcttgagca gaaaatgagc
    22921 atggcgcaaa ccgcgcacag ccagtttgag caggcttatc agctggtggt ggcaatcaac
    22981 ggcccgctgg cgcgtaacga ggcgtgggat gtcgcccgcg aactgctgcg cgaaggggtc
    23041 gagcagcgtc acctggcaga gcaggttcaa ccgttgcgga tgcgcttaag tgaactggaa
    23101 cagcgtctgc gcgagcagca agaagccgaa cgcctgctgg cagatttctg caaacgtcag
    23161 ggcaagaatt ttgatatcga cgaactggaa gccctgcatc aggagctgga agcgcgtatt
    23221 gcctctcttt ccgatagcgt gtctaacgcc cgcgaagagc gtatggcact gcgccaggag
    23281 caggaacagc tgcagtctcg cattcagagt ttgatgcagc gtgcaccggt ttggctggca
    23341 gcgcaaaaca gtctcaacca gttgagtgaa cagtgcggcg aagagttctc ctccagccag
    23401 gacgtcactg aatatctgca acagttgctg gagcgtgagc gcgaggcgat tgttgaacgc
    23461 gatgaagtgg gcgcgcgcaa aaacgctgtc gatgaagaga tcgaacgttt aagccagcct
    23521 ggcggctctg aagaccagcg tctgaatgcg ctggcggagc gttttggcgg cgtgctgctg
    23581 tcagaaattt atgacgacgt tagcctggaa gatgcgccgt acttctcagc gctgtatggc
    23641 ccgtcacgcc acgccatcgt ggtgccagat ctgtcacagg taaccgaaca cctggaaggc
    23701 ttgaccgatt gcccggaaga tctctatttg atcgaagggg atccgcagtc attcgatgac
    23761 agcgtgttca gcgttgatga gctggaaaaa gcggtagtgg tgaaaatcgc cgatcgtcag
    23821 tggcgttatt cacgtttccc ggaagtgccg ctgtttggtc gtgctgcgcg tgaaagccgt
    23881 attgaaagcc tccatgccga gcgtgaagtg ctttccgaac gcttcgccac gctctccttt
    23941 gatgtacaga aaacccagcg tctgcatcag gcgttcagcc gctttatcgg cagtcatctg
    24001 gcggtggcgt ttgagtctga cccggaagca gaaatccgtc aactgaacag ccgtcgcgtc
    24061 gaactggagc gggcgttgag taatcatgaa aatgataacc agcagcagcg tattcagttt
    24121 gagcaggcga aagaaggcgt tacggcgctg aaccgcattc tgccgcgtct caacctgttg
    24181 gctgatgaca gcctggcgga tcgcgtcgat gaaatccgcg aacgtctgga tgaagcccag
    24241 gaagccgcgc gttttgttca gcagtttggc aatcaactgg cgaaactgga accgattgtt
    24301 tcagtgttgc agagtgaccc ggaacagttc gaacagttaa aagaagatta cgcgtattct
    24361 cagcagatgc agcgcgatgc ccgtcagcag gcgtttgccc tgacggaagt ggtgcagcgt
    24421 cgtgcgcact ttagctattc tgactcggca gaaatgctta gcggtaacaa cgatctcaac
    24481 gaaaaactgc gtgaacgtct ggaacaggcg gaagcggagc gtacccgcgc tcgcgaagcg
    24541 ttgcgcggtc acgcagcgca gttgagtcag tacaaccagg tgctggcttc gctgaaaagt
    24601 tcttacgaca ccaaaaaaga gctactcaac gatctgcaac gtgaattgca ggatatcggc
    24661 gtgcgtgctg atagcggggc agaagagcgg gcacgtattc gccgtgacga gctgcatgca
    24721 caactgagca ataaccgttc gcgccgcaat caactggaaa aagcgctcac cttctgcgaa
    24781 gcggagatgg acaacctgac ccgcaaactg cgcaagctgg agcgtgatta ctttgagatg
    24841 cgcgagcagg tagtgaccgc gaaagcgggc tggtgtgcag tcatgcgcat ggtgaaagat
    24901 aacggcgttg aacgccgctt acaccgtcgt gagctggctt atctctccgc tgatgatttg
    24961 cgttccatgt cggataaggc gttaggtgcg ctgcgtctgg cggtggcgga taacgaacat
    25021 ctgcgcgacg tgctgcgcat gtcggaagat ccgaaacgtc cggagcgtaa aattcagttc
    25081 ttcgtggcgg tttatcagca tctgcgtgaa cgtattcgtc aggatattat tcgtaccgat
    25141 gatccggtgg aagctatcga acagatggag atcgaactta gccgtctgac cgaagaatta
    25201 acctcccgtg aacagaaact ggcgatcagt tcccgcagcg tggcgaacat cattcgcaaa
    25261 accattcagc gcgaacagaa tcgtatccgt atgctcaacc aggggttgca gaacgtatcg
    25321 tttggtcagg tgaacagcgt acgtctgaac gtgaacgtgc gtgaaacgca cgccatgctg
    25381 ttggatgtgc tctctgaaca gcacgagcag catcaggatc tgtttaacag caaccgtctg
    25441 accttctcgg aagcgctggc gaaattgtat cagcgtctta atccgcagat tgatatgggg
    25501 cagcgcacgc cgcagaccat tggcgaagaa ctgctggatt accgcaacta tctggaaatg
    25561 gaagttgagg ttaaccgtgg ttccgatggc tggttgcgcg cagagtctgg tgcattgtcg
    25621 accggtgagg cgattggtac cggtatgtcg attctggtga tggtggtaca aagctgggaa
    25681 gatgaatctc gccgcctgcg cggtaaagat atctctcctt gccgcctgct gttcctcgat
    25741 gaagcagcgc gactggatgc tcgttctatc gccacgctgt ttgaattgtg tgaacgtttg
    25801 caaatgcagc tcatcatcgc agcgccggaa aatatcagtc cggaaaaagg caccacctat
    25861 aaactggtac gtaaagtctt ccagaatacc gaacatgttc acgtcgtcgg cctgcgagga
    25921 tttgcgccgc aactccctga aacgcttcca ggaagtgacg aagcgccttc ccaggcgagt
    25981 taaaatgaag gcggcagcaa tgccgccttt tctttttccg aaaactccgt ttctgcacta
    26041 aaaaagtggc acattacggc gcgcttttct ttaaacttct ttacattagg ttatgtaaaa
    26101 acgtatcggc gtttatatac tgaagttaag cctgatgagt aacaggcttg ctcgtcatac
    26161 tttcgtgagt attggcgttg tacaggcaag tcgtaaaata acagcctggc tattcagagt
    26221 atgataaaaa cagggggcaa gggatgttgc ttaatatgat gtgtggtcgt cggctgtcgg
    26281 caatcagttt gtgcctggcc gtaacattcg ctccactgtt caatgcgcag gccgatgagc
    26341 ctgaagtaat ccctggcgac agcccggtgg ctgtcagtga acagggcgag gcactgccgc
    26401 aggcgcaagc cacggcaata atggcgggga tccagccatt gcctgaaggt gcggctgaaa
    26461 aagcccgcac gcaaatcgaa tctcaattac ccgcaggtta taagccggtt tatcttaacc
    26521 agcttcaact gttgtatgcc gcacgcgata tgcaacccat gtgggaaaac cgtgatgctg
    26581 ttaaagcctt ccagcaacag ctggcagagg tggcgattgc tggtttccag ccgcagttta
    26641 ataaatgggt agagttactg accgatcctg gtgttaacgg gatggcacgt gacgtggtgc
    26701 tctctgatgc gatgatgggc tatctccatt tcattgcaaa tattccggtc aaaggcactc
    26761 gctggctata tagcagtaaa ccttatgcgc tttcaacgcc gccgctttcg gtgattaacc
    26821 aatggcagct agcgctggat aaaggtcaat tgcctacgtt tgttgcagga ctggcaccgc
    26881 agcatccgca atatgcggcg atgcatgagt cgttactggc cttactcagt gacaccaaac
    26941 cgtggcccca actgaccggc aaagcaacgt tgcgcccagg gcagtggagt aacgacgtac
    27001 cggcgttgcg cgaaatattg caacgcacag gcatgttgga cggggggccg aaaattacgc
    27061 tacctggcga tgacacgcca actgacgcgg tagtcagccc atccgctgtt actgttgaaa
    27121 cagcagaaac taagccgatg gacaagcaaa cgacgtctcg tagtaaacct gcgcctgccg
    27181 ttcgtgccgc ctacgataat gaactggtgg aagccgttaa acgttttcag gcatggcaag
    27241 gattgggggc agatggtgct attggcccgg caacgcggga ctggttaaac gtaacgcccg
    27301 cccagcgtgc tggcgtgttg gcactcaaca tccagcgatt gcgcttgctg ccaacagagc
    27361 tttctaccgg gatcatggtt aacattccgg cctattcgct ggtctactat cagaacggca
    27421 atcaggtgct ggattcgcga gtcattgtcg gtcgccccga tcgcaaaacg ccgatgatga
    27481 gcagtgccct taacaacgta gtggtaaacc cgccgtggaa cgtacctcca actctggcac
    27541 gcaaagatat tctgccaaaa gtgcgcaacg atccgggata tctcgaaagc catggctata
    27601 cggtgatgcg cggctggaac agcagagaag cgattgaccc atggcaggtt gactggtcta
    27661 caatcacggc ctcgaattta ccgttccgct tccagcaggc tccaggccca cggaactcgc
    27721 tggggcgcta taaattcaat atgccgagtt cagaggccat ttatttgcat gacacgccga
    27781 accacaatct gttcaagcgt gatacacgcg cattgagctc aggctgtgta cgagtgaata
    27841 aagcttccga tctggcgaat atgctgttgc aggatgcagg ctggaatgac aaacgtattt
    27901 ctgatgcgct gaagcagggt gatacacgtt acgtcaatat tcggcagtcg attccggtga
    27961 atctctacta cctgacggcc tttgttggtg cagatggtcg tacccagtat cgtacagata
    28021 tttacaatta tgatctgcct gcgcgatcca gctcgcaaat cgtatcgaaa gcggaacaat
    28081 taatcaggta aatgaagtag ttcggggaaa tcagttgtcg taataattag catgattggg
    28141 ggtgattctc tgcagacccc gtcaatgctg gggttgagtc atcttgacgt ctgctttacg
    28201 ggcggttaag gtgcctcttg tgcgccagaa gtgcatataa acgataacat tgacctgtag
    28261 acttgattat catggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg
    28321 ttgcactcgg tgccgccatc ctgccgaccc ctgcgtttgc aacactctct accccacgcc
    28381 cgcgcatttt gacactcaat aatcttcata ccggagagtc aatcaaagcg gagtttttcg
    28441 atggcagagg ctatattcag gaagaattgg caaaacttaa ccattttttc cgcgattacc
    28501 gcgcgaacaa aataaagtcc atcgacccag gattattcga ccagttgtat cgcctgcaag
    28561 ggttgttagg cacgcgcaaa ccggtgcaac tcatttccgg ttatcgttcc attgatacca
    28621 acaatgaact acgcgcccgc agccgtggag tagcgaagaa aagctatcac actaaaggcc
    28681 aggcgatgga tttccatatt gaaggtatcg cgttaagcaa tattcgcaaa gccgcgttat
    28741 ctatgcgcgc aggtggtgta ggatattacc cacgtagtaa ctttgtgcat attgataccg
    28801 ggccagcacg gcactggtag taattgctta acgaaacagg ggcagtatga actatcgtat
    28861 tattccggtc accgcattct cccagaactg ttcattgatc tggtgtgaac aaacccgcct
    28921 ggccgcactg gtcgatcctg gcggcgatgc ggaaaaaatc aaacaggaag ttaatgacag
    28981 cggcctgaca ctgatgcaga tcctgctgac gcatggtcat ctggaccacg ttggcgcagc
    29041 ggcggaactg gcgcaacatt acggcgtgcc ggtttttggc ccggaaaaag aagatgagtt
    29101 ctggctgcaa ggcttgcctg cgcaaagtcg tatgtttggt ctggaagagt gtcagccgct
    29161 gacgccagat cgttggctga aagaaggcga taccatcagc atagggaatg tgactttaca
    29221 ggtgttacat tgccctgggc atacgccggg tcatgtcgtg ttttttgatg atcgggcaaa
    29281 gctgctgatt tctggcgatg ttattttcaa aggcggagta gggcgcagtg acttcccgcg
    29341 tggcgatcat aatcaactga tttcttcaat caaagataaa ttgctgccac tgggggatga
    29401 cgtgacattt attccgggtc acggaccatt atccacactt ggttatgaac gcctgcataa
    29461 tcccttcctg caagacgaaa tgcccgtctg gtaaggcaca cataaaaaag cccgcttttc
    29521 agcgggcttc attgttttta acgcttacag cactgccaca atcgcttcgc acagcggagc
    29581 catgttatct ggtgtcatcc cggccacgtt tacgcgacca gaagcaacag catacacgcc
    29641 aaactcttcg cgcagacgca gcacttgttc ttttgtcagg ccactgaagg agaacatgcc
    29701 gttctgtttg atgataaagc tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac
    29761 gaacaactga cgcatacgct gaatacgctg gcgcatatca gtcagctctt gttcccaaat
    29821 cgcacgtaac gcatcgttgc tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg
    29881 gttagagtag ttagcgcgaa tcgccgcttt catttggctg aatgcgcgat caacggtttc
    29941 actgtcggca gcaaccagag tacaagcgcc aacacgctcg ttgtacaggc caaagttttt
    30001 agagtaggaa ctggcaacaa tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc
    30061 agcatcttct tccagaccac gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca
    30121 gcctttctca acggagagtt gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc
    30181 ggttgggttg tggcagcagc catggaacag cactacgtcg ccagcctgag cttcgttcag
    30241 gctgttaatc agtgcatcga agtcaagggt gtggttttcc gcatcataat aagcgtattc
    30301 acgaacttcc agacctgcag agttaaagac gctcttatgg ttcggccagc ttgggttgct
    30361 cacccacact cgcttaacgc tggtattttt tgccaggaaa tcggcagcta cgcgtagtgc
    30421 gccagtgcca cccggagtct gtgccgtgcg agcacgtttg tcattgatca gggcgctacc
    30481 tttaccaaac agcagttcct gagtgcagcg accaaattca gggatgccgt caatgccgag
    30541 gtaatttttg gtggtttcat tttcgagcag atactgttca gcctttttca cgctggtcag
    30601 taccggggtt ttgcccgtct catctttata gacaccaatc ccgaggttaa ttttgccggg
    30661 acgttcatcg gcacgaaaca gatcggccag gcccagaatc gggtcggcag gagcggcggt
    30721 aatgttctca aacatgacga ggttccatta tggttacaga agggaagtcc gctatcaggg
    30781 taacgggaga tttacaaaat tccaactatt actgatgaaa acgcaggctg tttttgcaag
    30841 acgtgagatt gctctggaag gcataaaaaa aacaggacca acgtcctgtt ttttcggcat
    30901 ttaacaaaga ggtgtgctat tagaactggt aaacgatacc cacagcaacg gtgtcgtcag
    30961 aacctacgcc cagtttgttg tcagaatcga tctggttgat gatgtagtca acataggtgg
    31021 acatgttttt gttgaagtag taggttgcgc ccacttcaaa gtagttcacc agatcaacat
    31081 caccgatacc ttctacgtct ttcgctttag atttggtgta agcgatggac ggacgcagac
    31141 cgaaatcgaa ctggtattgc gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc
    31201 tgatgtttgt aaatttatta gtgatcggcg tagcgttacg cgtttcaccg tagttcgctg
    31261 ccaggtagat gttgttcgca tcatacttca gaccagtagc ccactgttca gcttttttac
    31321 cgttgccaag aagttgagct tcttgcaggt tggtacggtc agccgcacca taagcaccaa
    31381 cgataccaaa gccttcgtat tcgtagctga tagaaccgcc aacaccgtcg ccgttagagc
    31441 ggcgtgcagt gtcacgctcg tttttaccca ggtactgaac agcgaagttc aggccatcaa
    31501 ccagaccaaa gaagttggag ttacgataag tagcaacgcc gccaacacga ccaacgaaga
    31561 agtcatcgct gtatgcagta tcaccaccaa attctggcag catatcggtg taacccagtg
    31621 catcataaac cacaccgtag ttacggccgt aatcgaaaga accaacgtca gcgtatttaa
    31681 gacccgcgaa tgccagacgc gttttgttac cagtttgagc gtcagtgcct tcagagttgt
    31741 taccctggaa gttatattcc cactgaccat aaccggtcag atcggaattg atttgagttt
    31801 cccctttaaa accaagacgg gcataggtca tgtcgccatt gccaccgtaa ctgttttcac
    31861 cgttaccctt ggagaaataa tgcagaccga cagctttacc gtacagatct actttgttgc
    31921 catctttgtt atagatttct gcagcgtttg cagtacctgc tactaacaga gcagggacga
    31981 tcactgccag aatattgcgc ttcatcatta tttattaccc tcatggtttt ttttatgaca
    32041 cctgccactg ccgtcaataa gttctgtcaa taaaaattta cggaactatt gatgagagtt
    32101 tggtgtcttt atgtgtctgc aggcatcttt ccattcaaac t
//
LOCUS       DAZUGK010000032        31070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZUGK010000032 DAZUGK010000000
VERSION     DAZUGK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.032"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..230
                     /gene="tuf"
                     /locus_tag="ACHY5W_001329"
     CDS             <1..230
                     /gene="tuf"
                     /locus_tag="ACHY5W_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4446258.1"
                     /translation="HTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTL
                     IHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            460..843
                     /gene="secE"
                     /locus_tag="ACHY5W_001330"
     CDS             460..843
                     /gene="secE"
                     /locus_tag="ACHY5W_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4446259.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            845..1390
                     /gene="nusG"
                     /locus_tag="ACHY5W_001331"
     CDS             845..1390
                     /gene="nusG"
                     /locus_tag="ACHY5W_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4446260.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHY5W_001332"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHY5W_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4446261.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHY5W_001333"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHY5W_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4446262.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHY5W_001334"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHY5W_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4446263.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHY5W_001335"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHY5W_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4446264.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHY5W_001336"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHY5W_001336"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4446265.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHY5W_001337"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHY5W_001337"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4446266.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHY5W_001338"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHY5W_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFS4446267.1"
                     /translation="MKQEVDKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHY5W_001339"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHY5W_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFS4446268.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGRKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHY5W_001340"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHY5W_001340"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFS4446269.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCTAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHY5W_001341"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHY5W_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFS4446270.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGVALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHY5W_001342"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHY5W_001342"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFS4446271.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHY5W_001343"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHY5W_001343"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFS4446272.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVHWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLPRAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHY5W_001344"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHY5W_001344"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFS4446273.1"
                     /translation="MSATKLTRREQRTRAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19436..19912)
                     /gene="rsd"
                     /locus_tag="ACHY5W_001345"
     CDS             complement(19436..19912)
                     /gene="rsd"
                     /locus_tag="ACHY5W_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFS4446274.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20007..20780
                     /gene="nudC"
                     /locus_tag="ACHY5W_001346"
     CDS             20007..20780
                     /gene="nudC"
                     /locus_tag="ACHY5W_001346"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFS4446275.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20820..21884
                     /gene="hemE"
                     /locus_tag="ACHY5W_001347"
     CDS             20820..21884
                     /gene="hemE"
                     /locus_tag="ACHY5W_001347"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFS4446276.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21894..22565
                     /gene="nfi"
                     /locus_tag="ACHY5W_001348"
     CDS             21894..22565
                     /gene="nfi"
                     /locus_tag="ACHY5W_001348"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFS4446277.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22608..23198
                     /gene="yjaG"
                     /locus_tag="ACHY5W_001349"
     CDS             22608..23198
                     /gene="yjaG"
                     /locus_tag="ACHY5W_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFS4446278.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23385..23657
                     /gene="hupA"
                     /locus_tag="ACHY5W_001350"
     CDS             23385..23657
                     /gene="hupA"
                     /locus_tag="ACHY5W_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFS4446279.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23670..24365
                     /gene="yjaH"
                     /locus_tag="ACHY5W_001351"
     CDS             23670..24365
                     /gene="yjaH"
                     /locus_tag="ACHY5W_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFS4446280.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24367..24786)
                     /gene="zraP"
                     /locus_tag="ACHY5W_001352"
     CDS             complement(24367..24786)
                     /gene="zraP"
                     /locus_tag="ACHY5W_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFS4446281.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRSTGMGYGGCGGGGHMGMGHW"
     gene            25024..26400
                     /gene="zraS"
                     /locus_tag="ACHY5W_001353"
     CDS             25024..26400
                     /gene="zraS"
                     /locus_tag="ACHY5W_001353"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFS4446282.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGRVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26397..27722
                     /gene="zraR"
                     /locus_tag="ACHY5W_001354"
     CDS             26397..27722
                     /gene="zraR"
                     /locus_tag="ACHY5W_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFS4446283.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLTGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27719..29008)
                     /gene="purD"
                     /locus_tag="ACHY5W_001355"
     CDS             complement(27719..29008)
                     /gene="purD"
                     /locus_tag="ACHY5W_001355"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4446284.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29020..30609)
                     /gene="purH"
                     /locus_tag="ACHY5W_001356"
     CDS             complement(29020..30609)
                     /gene="purH"
                     /locus_tag="ACHY5W_001356"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4446285.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALTITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7343 a         8049 c         8491 g         7187 t
ORIGIN      
        1 gtcatactcc gttcttcaaa ggctaccgtc cgcagttcta cttccgtact actgacgtga
       61 ctggtaccat cgaactgccg gaaggcgtag agatggtaat gccgggcgac aacatcaaaa
      121 tggttgttac cctgatccac ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg
      181 aaggcggtcg taccgttggc gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa
      241 catttgacgc aatgcgcact aaaagggcat catttgatgc cctttttgca cgctttcgta
      301 ccagaacctg gctcatcagt gattttcttt gtcataatca ttgctgagac aggctctgtt
      361 gagggcgtat aatccgaaaa gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg
      421 ggtgaaaatg tttgtagaaa acttctgaca ggttggttta tgagtgcgaa taccgaagct
      481 caaggaagcg ggcgcggcct ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg
      541 gtggcgattg tcggcaacta cctttatcgc gacattatgc tgccgctgcg tgcgctggcc
      601 gtagtaattc tgattgctgc agcgggtggt gtcgcgctgt taacaacaaa aggtaaagct
      661 accgttgctt ttgcccgtga agcgcgtacc gaagtccgta aggtcatttg gccgactcgc
      721 caggaaacat tgcacaccac gctgattgtg gctgcggtta ccgcagtaat gtcactgatc
      781 ctgtggggac tggatggtat tctggttcgc ctggtatcct ttatcactgg cctgaggttc
      841 tgagatgtct gaagctccta aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga
      901 aggccgcgta gcaacgtcgc tgcgtgagca tatcaaatta cacaacatgg aagatttgtt
      961 tggtgaagtc atggtaccaa ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa
     1021 aagcgaacgt aaattcttcc ctggctacgt cctcgttcag atggtgatga acgacgcgag
     1081 ctggcacctg gtgcgcagcg taccgcgtgt gatgggcttc atcggcggta cttccgatcg
     1141 tcctgcgcca atcagcgata aagaagtcga tgcgattatg aaccgcctgc agcaggttgg
     1201 tgataagccg cgtccgaaaa cgctgtttga accgggtgaa atggtccgtg ttaatgatgg
     1261 tccgttcgct gacttcaacg gtgttgttga agaagtggat tacgagaaat ctcgtctgaa
     1321 agtgtctgtt tctatcttcg gtcgtgcgac cccggtagag ctggacttca gccaggttga
     1381 aaaagcctaa cccagcgatc aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt
     1441 ggaatacaat ttcgcgcctt ttgtttttac gggccttgcc cgtaaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagaactg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaacgaatga agccaacgcc gccacaagct gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgt
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca ttgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagcg gaaagcctgt ttgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggtgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ctgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgtg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggttgc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta ttaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tatattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcgtct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ttgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaggaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttctgg tcagatccgc
     7981 ctgtacgacg gccgcactgg tgaacagttc gaacgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt actaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggccta ctgctcgata tgccgctgcg cgatattgaa cgcgtactgt acttcgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaaa tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaactaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggata tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtgggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagct cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggctttg catatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa cggcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtat tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcaaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccaca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tccctggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gtggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt attaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaggcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atccctgcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggataagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagcctttgc gaggcgcgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cagcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagccg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacat ggacgcaggc gtgggaagga gacagagtag cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt ctgcaaccat atagcagtca acgcgccagc
    14281 tgtcggaaag gccaattagc gcgccgaggc caatcttatc aatccccgca cggcccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgttttct gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagttgct
    14461 ttaactcggc gtattccgtc tccgccagcg gctgtacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgctctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca taccgatgcc tccagaaatc cggtcagcgg
    15001 gctggtggca tgagcaaaat aactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctact gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aacagtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagcc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgtacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacatct caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gtccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccacgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgacttacc gtcgaacagt
    16021 cggagttctc ccgcaggcgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taactccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcttgtg caaccgcatc ttttaacgcc tcacccgtta atcgttgttg taatgctgtc
    16381 agttgaatat cgggattcaa ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtatagcgc agcaggtgta
    16561 cccagcccgc caagaccgat aatcaacacc tggctgtcga gcagtttttg ctgcccgtca
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgcgg
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc aggcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 ggtcgaaacg cccagccgca gaccagccgc acggatggca ctgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccaat gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaga atttcggccc acacatggag
    17461 caaaaatggg cgactttacc tgactcttgc ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca catcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgcgagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagcacccc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcactg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgctgagttg
    18481 ccgatattgg cgttaacttt taccaggaag ttacggccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgttttg
    18781 gcgcgttttg gcgttaataa gccgctaaaa cgtagctcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagctt tgccagccct tgctgcacgt taatggcgat ctgcggatcg
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccgggttgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggtgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcaa aggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc agaactcaag
    19441 caggatgttt gacgcgggca gcgtcaagca ccagcagaat cagcttatct tccagcacaa
    19501 agcgcgcttc cagcgcctcg ccgatgtcag ataaaacctg ttgaaactca aggtaattat
    19561 catgatcgat agcggtttcc aggctggaat cgtagtaatc cataatctgt tgggtattgg
    19621 cttcgagttg cggccaaatc ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat
    19681 gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac
    19741 aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc
    19801 caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag cgatcaacca
    19861 gtttgttact tcctctgacg cgctccgtca ggttatcgag ctggttaagc atgattgact
    19921 ccgcaagttt gtattcaaaa actgctcagt gagaaatgta aaaaccatgt taaacatgcc
    19981 agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta gatcacggct
    20041 ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg ccatatggcg
    20101 aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg
    20161 aacctgtttg gttaatacaa cagcagcggc gtcacgatat ggggtcggta cgtcaggtca
    20221 ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg gcggagtttt
    20281 accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc aaaaccgaat
    20341 gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta
    20401 ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc catcgtaacg
    20461 gtgtccatac ggtacttgcc ggattcgtcg aagtgggcga aaccctcgag caggcggtcg
    20521 cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttat gtgacttccc
    20581 agccgtggcc gtttcctcag tctttaatga ccgcgtttat ggcggaatat gacagcggcg
    20641 acatcgtgat cgacccgaaa gaattgctcg aggcgaactg gtatcgctat gacgatttgc
    20701 cgttactccc gccgcccggc accgtggcgc gccgtctgat agaagatacg gtggcgatgt
    20761 gtcgggcaga gtacgagtga tgatacactg accgcctgac gcactaagga acagccaaaa
    20821 tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc gttgatgtca
    20881 ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa gccacgcgcg
    20941 cccaggcggg cgattttatg tcgctgtgca aaaacgccga gctggcgtgt gaagtgacct
    21001 tgcaaccgct gcgtcgctac ccgctggatg cggcgatcct cttttccgat atcctcaccg
    21061 tcccggacgc gatggggtta gggctctatt ttgaagccgg agaaggtccg cgttttacct
    21121 cgccagtcac ctgcaaagct gacgtcgata aactgccaat tccggacccg gaagatgaac
    21181 tggggtacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc
    21241 tgattggttt ttccggcagc ccgtggacgc tggcaaccta catggtggaa ggcggcagca
    21301 gcaaagcgtt caccgtgatc aaaaaaatga tgtatgccga tccgcaggcg ctgcacgcgc
    21361 tactcgataa actggcgaaa agcgtcaccc tatacctgaa cgcgcagatt aaagccggtg
    21421 ctcaggctgt gatgattttc gacacctggg gcggcgtgct taccgggcgc gattatcaac
    21481 agttctcgct ttattacatg cataaaattg ttgatggttt actgcgtgaa aacgacggtc
    21541 gccgcgtacc ggtcacgctg tttaccaaag gcggcggaca gtggctggaa gccatggcag
    21601 aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat gcgcgccgcc
    21661 gtgtgggcaa taaagtcgcg ttgcagggta atatggatcc gtcgatgctg tacgcgccgc
    21721 ctgcccgcat tgaagaagaa gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc
    21781 atgtctttaa ccttggtcac ggcattcatc aggatgtgcc gccagaacat gctggtgtgt
    21841 tcgtggaggc agtgcatcga ctgtctgaac agtatcaccg ctaaggagcg attatggatc
    21901 tcgcgtcatt acgcgctcaa caaatcgaac tggcttcttc tgtgatccgc gaggatcgac
    21961 tcgataagga tccgccggat ctgatcgccg gagccgatgt cgggtttgag cagggcggag
    22021 aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg gtggagtata
    22081 aagtggcccg catcgccacc accatgcctt acattccagg ttttctttcc ttccgcgaat
    22141 atcctgcgct gctggcagcg tgggagatgc tgtcacaaaa gccggattta gtgtttgtcg
    22201 atggtcatgg gatctcgcat cctcgccgtc ttggcgtcgc cagccatttt ggtttgctgg
    22261 tggatgtgcc gaccattggc gtagcgaaaa aacgactatg cggtaaattc gaaccgctct
    22321 ccagcgaacc gggcgcgctg gccccactga tggataaagg cgagcagctg gcctgggtct
    22381 ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg
    22441 acagcgcgct ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg gagccaacgc
    22501 gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc
    22561 cctaattcag gtaaactgcg ggtaatttcc gtatttgaga actcaacatg ttacaaaacc
    22621 caattcatct gcgtctggag cgcctggaaa gctggcagca tgtcactttc atggcttgct
    22681 tatgcgaacg catgtacccc aattacgcca tgttctgcca gcaaaccggt tttggtgatg
    22741 ggcaaattta ccgtcgtatt ctcgatctca tctgggaaac gctgaccgtt aaagatgcaa
    22801 aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca gccgacgatt
    22861 tcgatctgta cggcgtttat ccggcaatcg atgcctgtgt ggcgttaagt gaactggtcc
    22921 attcgcgttt gagtggtgaa acgctcgaac acgcggtgga agtgagtaag acctccatca
    22981 cgaccgttgc gatgctggaa atgactcagg ctggtcgcga aatgagcgat gaagagctca
    23041 aagaaaaccc agctgtagag caagaatggg atattcagtg ggaaatattc cgacttttag
    23101 ctgagtgcga agaacgcgat atcgagctga taaaaggcct tagggcagac ctgcgtgagg
    23161 cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg tgatttaacg
    23221 cctgatttgt cgtacctgga gtcttccctt tcgccccccg tctggtctac atttgggggg
    23281 cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc atttccgtcg
    23341 cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac aagactcaac
    23401 tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg
    23461 agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta caactggttg
    23521 gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg
    23581 gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc aaggcactga
    23641 aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg gtggtttcac
    23701 cccttttgtc tttctggcgt cgatcattga tgctggcagg tgcgctgttt ctcactgcct
    23761 gtagccacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa gaccagggcg
    23821 cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc
    23881 cgtggcgcag tggcgatacc acgacgcgag agtatcgctg gcagggcgat aacctcacgc
    23941 tcatcaatat caatgtttac agcaaacccc cggtgaatat tcgcgcgcgt tttgacgatc
    24001 gcggcgattt gagctttatg caacgcgaat ccgatgggga aaagcagcag ctttctaacg
    24061 accaaatcga tttataccgt tatcgtgctg atcagatccg ccagattagc gatgccttac
    24121 gtcaggggag agtcgtgctg cgccaggggc gctggcatgc gatggaacag accgtgacca
    24181 cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg catatcgagc
    24241 gccgccagag ccgctcttct gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt
    24301 cgcaattact gttggtggca aactctgatt tctgtcgctg gcaacccaac gagaaaacgt
    24361 tctgatttac cagtggccca tacccatatg accgccacca ccgcagccgc cgtaacccat
    24421 tccggtactg cgcggaatac ccgcttcagc catcgcaata tctcgtttca cccgtaactc
    24481 atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt tgctgctatc
    24541 cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca gttgctgttg
    24601 cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg tctgttgttc
    24661 gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag caaatgcaga
    24721 tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt tcgtgttccg
    24781 tttcatggat aatcctccag tggttgtctt acttcgggta ttgcatcttc cgtgccaacg
    24841 atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg actcgcctgc
    24901 ggcgggtagc gagtcatttt tactcattga aaattaagcc tttgtgttac agcgcagggt
    24961 aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca ggcaaagaag
    25021 aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag cgcgatcctc
    25081 cccgtggtca ttgttgggct ggtagggtta tttgcggtga cggtgattcg cgattatggg
    25141 cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gccgtgtact tattcgcgct
    25201 cttgaatccg gctcacgcgt cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc
    25261 ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa
    25321 ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta ttccccgcag
    25381 gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga tagcgcagac
    25441 ggtgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc tggaatgcac
    25501 cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat cgctttcgac
    25561 gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat tatcctcttc
    25621 gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg ccgctatctg
    25681 cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt ggcgctgggg
    25741 catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat taaaggactg
    25801 gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg
    25861 atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agctgctgga actggttaag
    25921 ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca ctcattacag
    25981 ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc caacgacaca
    26041 ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa tctctatctc
    26101 aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag cgaaagcggc
    26161 gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga tcagcttgat
    26221 gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg gctggcggtc
    26281 gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca ggagggaaaa
    26341 ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc acaaggatga
    26401 cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc actattttgc
    26461 aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg cgacaggcgc
    26521 tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga atggcggaga
    26581 tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt ccggtgctga
    26641 ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact ggggcgctgg
    26701 attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa aaggcgctcg
    26761 cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag ttcggtatgg
    26821 tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg
    26881 aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc gccagggcga
    26941 ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt gcggcactta
    27001 acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt accggagccg
    27061 ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa
    27121 ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag
    27181 ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt gcggcgaccc
    27241 atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc
    27301 tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat atccctctgc
    27361 tgactggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta aaaggcttta
    27421 cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt cgtgaactgg
    27481 aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc gaacgcgagc
    27541 tgccgctggc gattgccggt acgccgatcc cgctggggca aagtcaggat attcagccgc
    27601 tggtggaagt cgaaaaagaa gtgattctgg cggcactgga gaaaacgggc ggcaacaaaa
    27661 ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt
    27721 agttctgctc gcgttcgata gcgcgccagc cgatatcttt ccggcagaag cagtcgtccc
    27781 agtgaatatc ggtcattaag gcataagcgc gtttctgcgc ttctgccacg gtatgaccca
    27841 gcgcggtgac gcacagtacg cgcccgccgc tggtcactac ctgctcgtta tccgccagtt
    27901 tcgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg ccgtggatca
    27961 catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg ccgagagaag
    28021 cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga
    28081 gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga tcgccaaagc
    28141 ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg cccgcgtaga
    28201 gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc cagataatac
    28261 gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag taagcgccca
    28321 tcccgcccgt gtttggtccg gtatctttat cccctacgcg tttgtgatcc tgactggtag
    28381 ccatcggcag cacatgctct ccgtccacca tcacgataaa gctcgcttct tcgccatcga
    28441 ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc gccagcatat
    28501 cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct ttcccggcag
    28561 ccagaccgtc cgctttaatg acgattggcg cgcctttctc gcgcagatac gccagcgcag
    28621 gttctatctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg gccaggaaat
    28681 ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg ccgaagattt
    28741 tcagcccggc agcgcggaag gtatcgacca cgcctttcac cagcggcgct tccgggccga
    28801 cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg atatcggtca
    28861 cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa
    28921 caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc
    28981 cgccgttacc aatcactaat actttcatct attgctccat taatggcgga agtggcgcat
    29041 gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg
    29101 gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg catcaatacc
    29161 gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt ccaggccttc
    29221 atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct ggcccgcgcc
    29281 aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca cgaacttcgc
    29341 cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac gttgggtgac
    29401 gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca gcagaccgcc
    29461 gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc aggtcagaac
    29521 gcgtacgttc tgcttggcgg cggtgattgt cagggcttct tcgctggcgg aaggcgcaat
    29581 aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg
    29641 gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta
    29701 agcatcaaga atggaattgc cgatagccac gccgcaaggg ttggcgtgct tcacaatcac
    29761 acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt
    29821 gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg cttctttcac
    29881 attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac gcatatcctg
    29941 cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc
    30001 accgtggtaa gccggaacca tgctgccgaa gtagttggca atcatgctgt cgtaggcggc
    30061 agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc
    30121 gttggcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca cgattgcgac
    30181 atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat cgatgttctc
    30241 aaccgcatct tccagcgagc agccttcacg ggccacggtc tgggcgaacg gatacaggtt
    30301 aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat cgtcctgacc
    30361 gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc gtccatccat
    30421 catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt tttctgctaa
    30481 cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc
    30541 gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga ctggacgacg
    30601 ttgttgcatg gtaaatcccc tggatttgac tattacagag agcgttagct gaatttttcg
    30661 cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc attgtaacga
    30721 aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa ttttgacagt
    30781 ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc tgtgcgtaaa
    30841 aaggtataaa gcgggctttt gctggggaat gcagcagtca gtcatttttc tgcaattttt
    30901 ctattgcggc ctgcggagaa ctccctataa tgcgcctcca tcgacacggc ggatgtgaat
    30961 cacttcacac aaacagccgg ttcggttgaa gagaaaaatt ctgaaattca gggttgactc
    31021 tgaaagagga aagcgtaata tacgccacct cgcgacagtg agctgaaagc
//
LOCUS       DAZUGK010000033        31022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZUGK010000033 DAZUGK010000000
VERSION     DAZUGK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.033"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..795
                     /gene="artJ"
                     /locus_tag="ACHY5W_001357"
     CDS             64..795
                     /gene="artJ"
                     /locus_tag="ACHY5W_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFS4446286.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(846..2327)
                     /locus_tag="ACHY5W_001358"
     CDS             complement(846..2327)
                     /locus_tag="ACHY5W_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFS4446287.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQVETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRRSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2339..2659)
                     /locus_tag="ACHY5W_001359"
     CDS             complement(2339..2659)
                     /locus_tag="ACHY5W_001359"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4446288.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHYPH
                     LINQ"
     gene            complement(2681..4024)
                     /locus_tag="ACHY5W_001360"
     CDS             complement(2681..4024)
                     /locus_tag="ACHY5W_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4446289.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWVTVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(4304..5431)
                     /gene="rlmC"
                     /locus_tag="ACHY5W_001361"
     CDS             complement(4304..5431)
                     /gene="rlmC"
                     /locus_tag="ACHY5W_001361"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFS4446290.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEHQALAERFNDVPLWIRPQSFFQTNPVVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKI"
     gene            complement(5472..5960)
                     /locus_tag="ACHY5W_001362"
     CDS             complement(5472..5960)
                     /locus_tag="ACHY5W_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFS4446291.1"
                     /translation="MEDETLGFFKKTSSSHASLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6020..6865)
                     /gene="potI"
                     /locus_tag="ACHY5W_001363"
     CDS             complement(6020..6865)
                     /gene="potI"
                     /locus_tag="ACHY5W_001363"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFS4446292.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(6862..7835)
                     /gene="potH"
                     /locus_tag="ACHY5W_001364"
                     /pseudo
     CDS             complement(6862..7835)
                     /gene="potH"
                     /locus_tag="ACHY5W_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
     gene            complement(7845..8978)
                     /gene="potG"
                     /locus_tag="ACHY5W_001365"
     CDS             complement(7845..8978)
                     /gene="potG"
                     /locus_tag="ACHY5W_001365"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFS4446293.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLM
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9073..10185)
                     /gene="potF"
                     /locus_tag="ACHY5W_001366"
     CDS             complement(9073..10185)
                     /gene="potF"
                     /locus_tag="ACHY5W_001366"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFS4446294.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IFPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(10536..11012)
                     /locus_tag="ACHY5W_001367"
     CDS             complement(10536..11012)
                     /locus_tag="ACHY5W_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFS4446295.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11100..12002)
                     /gene="rimK"
                     /locus_tag="ACHY5W_001368"
     CDS             complement(11100..12002)
                     /gene="rimK"
                     /locus_tag="ACHY5W_001368"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFS4446296.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            complement(12063..12785)
                     /gene="nfsA"
                     /locus_tag="ACHY5W_001369"
     CDS             complement(12063..12785)
                     /gene="nfsA"
                     /locus_tag="ACHY5W_001369"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFS4446297.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(12769..13056)
                     /locus_tag="ACHY5W_001370"
     CDS             complement(12769..13056)
                     /locus_tag="ACHY5W_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFS4446298.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13216..13473
                     /gene="grxA"
                     /locus_tag="ACHY5W_001371"
     CDS             13216..13473
                     /gene="grxA"
                     /locus_tag="ACHY5W_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFS4446299.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(13503..13880)
                     /locus_tag="ACHY5W_001372"
     CDS             complement(13503..13880)
                     /locus_tag="ACHY5W_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFS4446300.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            14150..15835
                     /locus_tag="ACHY5W_001373"
     CDS             14150..15835
                     /locus_tag="ACHY5W_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFS4446301.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16168..16719
                     /locus_tag="ACHY5W_001374"
     CDS             16168..16719
                     /locus_tag="ACHY5W_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFS4446302.1"
                     /translation="MDINELKECLHLEVIGKSRKFTWRKVIVRAMKHRRVRYLFWWRI
                     AKYGHEKGGYWRKIAGKIERKILDSYDVKIPLVVDIGKGLDISYLTGVVIGHNVKIGE
                     NCSIKPGVTIGLRGHFDEMDIQIGNNVTIGCNASILGGKVYIGDNVTIGAHALVLHDI
                     PENSIFINKIEYEIIPKKVIAEM"
     gene            complement(16779..17315)
                     /gene="rcdA"
                     /locus_tag="ACHY5W_001375"
     CDS             complement(16779..17315)
                     /gene="rcdA"
                     /locus_tag="ACHY5W_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFS4446303.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17399..18607
                     /locus_tag="ACHY5W_001376"
     CDS             17399..18607
                     /locus_tag="ACHY5W_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446304.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            18607..19422
                     /gene="ybjI"
                     /locus_tag="ACHY5W_001377"
     CDS             18607..19422
                     /gene="ybjI"
                     /locus_tag="ACHY5W_001377"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFS4446305.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19508..19792
                     /gene="ybjH"
                     /locus_tag="ACHY5W_001378"
     CDS             19508..19792
                     /gene="ybjH"
                     /locus_tag="ACHY5W_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFS4446306.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(19833..21065)
                     /gene="mdfA"
                     /locus_tag="ACHY5W_001379"
     CDS             complement(19833..21065)
                     /gene="mdfA"
                     /locus_tag="ACHY5W_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFS4446307.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            21350..21946
                     /gene="ybjG"
                     /locus_tag="ACHY5W_001380"
     CDS             21350..21946
                     /gene="ybjG"
                     /locus_tag="ACHY5W_001380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4446308.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHFFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            22004..22762
                     /gene="deoR"
                     /locus_tag="ACHY5W_001381"
     CDS             22004..22762
                     /gene="deoR"
                     /locus_tag="ACHY5W_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFS4446309.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(22809..24011)
                     /gene="dacC"
                     /locus_tag="ACHY5W_001382"
     CDS             complement(22809..24011)
                     /gene="dacC"
                     /locus_tag="ACHY5W_001382"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS4446310.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LFTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24259..24885
                     /gene="gstB"
                     /locus_tag="ACHY5W_001383"
     CDS             24259..24885
                     /gene="gstB"
                     /locus_tag="ACHY5W_001383"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFS4446311.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(24882..25997)
                     /gene="yliI"
                     /locus_tag="ACHY5W_001384"
     CDS             complement(24882..25997)
                     /gene="yliI"
                     /locus_tag="ACHY5W_001384"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFS4446312.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRSTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPRWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26108..26491)
                     /gene="bssR"
                     /locus_tag="ACHY5W_001385"
     CDS             complement(26108..26491)
                     /gene="bssR"
                     /locus_tag="ACHY5W_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFS4446313.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQDEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26704..28029
                     /gene="rimO"
                     /locus_tag="ACHY5W_001386"
     CDS             26704..28029
                     /gene="rimO"
                     /locus_tag="ACHY5W_001386"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFS4446314.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28076..29404)
                     /locus_tag="ACHY5W_001387"
     CDS             complement(28076..29404)
                     /locus_tag="ACHY5W_001387"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4446315.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIVQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(29412..>31022)
                     /locus_tag="ACHY5W_001388"
     CDS             complement(29412..>31022)
                     /locus_tag="ACHY5W_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFS4446316.1"
                     /translation="IIDSVDTEYIEKTSAVSNNALFEIYFYYPGGNLLNASDKLFYLP
                     FAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIIS
                     IKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEA
                     VEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVN
                     DFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLN
                     EIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKF
                     ITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERF
                     TTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKR
                     FYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKL
                     GCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         8045 a         7987 c         7325 g         7665 t
ORIGIN      
        1 cattgttatc ccatgccgca gacacggcca ataatcataa gattgacaga cgggagttcc
       61 atcatgaaaa agttagttct tgccgcatta cttgcttcct ttactttcgg tgcttctgcc
      121 gcagagaaaa tcaattttgg cgtttcagcc acctatccac cctttgaatc tataggtgct
      181 aataatgaga ttgtcggctt tgatatcgat ctggcaaaag ccttgtgcaa acaaatgcag
      241 gcagaatgta cttttactaa tcacgcgttc gacagcctga tcccgtcact gaaattcaga
      301 aaatatgacg ccgtaatctc cggtatggat atcaccccgg agcgtagcaa acaggtatcg
      361 tttaccacgc cctactatga aaactcagcc gtcgtgattg ccaaaaaaga tacctacaaa
      421 acatttgccg atctgaaagg caaacgtatt gggatggaaa acggcaccac gcaccagaaa
      481 tatattcagg atcagcaccc ggaagtgaaa actgtctcct atgacagtta tcagaatgcc
      541 tttatcgatc tgaaaaatgg ccgtattgat ggcgtatttg gtgacacagc ggtggttaat
      601 gaatggctga aaaccaaccc gcaactgggc gttgctactg aaaaagtgac cgatccgcaa
      661 tacttcggca ccggcctggg tatcgctgta cgtccggata acaaagctct gctggaaaaa
      721 ctgaataacg cgctggcagc aattaaagct gacggtacat atcagaaaat cagcgatcag
      781 tggttcccgc aataattatt catttgccgg agtgggcgaa cacccactcc ggcaaaatca
      841 cttctctacg ctaatcccag gcgctgaaac tgctctggcg gcgcatcatt atctttcatc
      901 aactgaatca gcaacgcgat catccgggac tcgatcacat catcatgaat cggatgtagc
      961 tgctgcggat cgttttgcag atcaaacagc aaatgaccaa actgattcaa ccccttatat
     1021 ttttcctgtg ccgggatgcg taatacctgt aaccctttgg taaaacgaaa cggtggggcc
     1081 agcgataaag cccgcatctc atccaccgga tagcggctgt tcattttgca ggacatcaac
     1141 gtgtagttgg caatatcatc ctcacgcccc ggcagcgctg cacgcatata cacataacgc
     1201 ccatcagtta cgttagcatg accactgaaa acaccaaaca tcgccgagcg gcgtactggt
     1261 tcatcgtgac tgaccgtttt cgccagcggt tgcccctgca catcgggcgg cactggctgt
     1321 tgaaaaaagt catacaacgt tggcgcccag tcgatcattt gtaccagtgc ctgtcgccgc
     1381 tctcctttta caccactgcg cgggtcccag ataaacaacg gattattggc aacttcgttg
     1441 taataaggca tctggttttt cgcccaccag ccatgctcac cgagtaaaaa gccgtggtca
     1501 gtgccgacaa tcagcatggt atctcgccac agatcgtgct catccatcag gtcgagaatg
     1561 cgccccaggt tgcgatcgca catcgacacc aacgaacgat accggcaacg gatgtgctcc
     1621 acggcctcgt cgctttcctg cacccggcca cgcggccagt cataatgcgg tccatcccag
     1681 ttatcttcat acaaggaaag atactcttcc gttgtgtgaa acggctcgtg cggatcaaac
     1741 gtttcgacct gcaataacca gttatcttcc gcgtgatttt tgtggataaa ggcacagcct
     1801 gcatcaaaca ctttagtttg tggaaaatcc gcttcctgct gaatatattc ccggtttgcc
     1861 cagtcatggc gccacagacc agaaacaccg ccaccggtct gcttttgcgg cacccgtagc
     1921 acctcgggaa taggcggttg cgcaacgctt gcatgccagt gatcgccttc ctgtccgcgc
     1981 acaatctccc acgaactgta gcgattatga taattcccgc cgccatcttc ccagtaatgc
     2041 aaatgatcgc ttatcaggtg ggtatagatc cccgcctttt taagcagctc cggcatggaa
     2101 tcgtcaaacg gttctaacgg cccccactcc cgatgcagga agttacatcg cccggtatgc
     2161 aactcccgcc gggcaggcat acacggcata ctgccgacgt aactgttttc aaaggtggcg
     2221 gcatgagccg ccagacgttg gaaattaggc gctttggtta acgcatcgcc ataaggtggc
     2281 agataacgtt tattcagact atcgaacagc agaataatcg ctctcattac gccctacctt
     2341 attgattaat taaatgcgga taatgttgct gaaccacttc ataaatttgc tgaccaatag
     2401 tttcaccgtc cataatattc tctatcggga tcagatattg cccttgtttc gggtcaagac
     2461 taaccacatc gagtaatttt ttggaaatag caaaaatatc cgcccagtcg gtaaatccgc
     2521 cgacttcacc tgcggcggta tgttccattt caactttgaa atcatatttg gtcagcgctt
     2581 ttttaacgac cgtttgtaac ataacgctac tgcctacacc gtaaccacag acaaacagaa
     2641 tattaattgt atctttcatt tttaaaccct taatatttaa ttaacccgca taaggaccaa
     2701 gatggaatgt tgcagcgata aatttcagaa cttcacaaat agcaggccat acggttaccc
     2761 agtcaaatat tcctgtccag ccgacgccat tttcagcaag cccggttaac ggaatagccc
     2821 acaccgtacc gaaggtctga ataataccca gcaagaacgt acagataatt acggaacggt
     2881 atccgcccat gcggtttgct aatacgccaa ttggaccgcc agagaaaaac agaggtacaa
     2941 agccaggcaa taccataatt gggctgtgaa ccagtaataa gatccccatc gaaaaaatgg
     3001 tgccaaaagt acagaagacg aagcctaacg ttgcggcatt gggtgaaaaa ggtaacagcg
     3061 ctgcaacatc gacagcagga atagcgttag gaataaagcg atcttgccag cctttaaacg
     3121 aaccgttaat ttcgcccacc atcatgcgca caccctgcag cagaatgacc atatacatgg
     3181 agaactgtaa gccaaggttg atgatataga tgtaccaggg cttgcctttc gccattaact
     3241 ggacattatc gatacccgtt gatagcaaga aaccaccgac aaacagcgtc atcacaatgg
     3301 cgatagccgt tacgttatgg ttaaaaatcg ccagccaccc gggaagtttc aggttttcag
     3361 catcgtgctt ttcagggtca ccaaatttat gggcgaattt ggagaaaaac cacaatccca
     3421 acatctggtt atgagcaatg gtaaaaccgg catctttggt aactttggca atgggcttaa
     3481 caatgagcgt ggtggagaac gcccagaata cacccgttaa tattccggca ataataatgg
     3541 atgtagtcca gctaaaaccc agccaaaaga caatcaacca taataccgcc tgagaatgtg
     3601 aaacaccggt attccctgtc aaaaatatcc ctttggcacc agtataccga ccaaataata
     3661 ccagcagcag gttggtaaaa aacgccagta caaatacata accgacataa ccaaaattat
     3721 cccccaacgc aatttgcgtt gccgctttca ttgcgtagga atccattaaa tatccgtgaa
     3781 caccataggc tttacttacg gcttctgcta ccggtttaaa tgttgcggaa aactggaaac
     3841 caccaaataa caccatcatt aatcccacca tggcgctgac catgccggta ataattttta
     3901 ttggcgattt cttatcaagg gcataaccaa tacccaccag caaaccaatt aaaattgccg
     3961 gttcagttaa aaacgtttga ataacatcta tgaatttggc aaacatcgtc ggaacgccct
     4021 ccataagcgt acatttccgt tgtcaatgca tgaaccttct gctgacgtta ataatgtctc
     4081 aggaaatttt gtgtttcgct aagcaatgcc tctgtatgta ttcggtaagt gcactgtagt
     4141 aatcaaaaca gttcaattaa agatcttatt tgattatttg tgatttgatt tgcaaaaata
     4201 gagcaatata taaccgtgat cgatcggtta ttattaatcg atcgatcacg attattattt
     4261 tgagtgaata gttgttgccg aaaaatttac tgccgtgatg tatttaaatt tttaccagca
     4321 gcgtcaacac ttcataatgc gcggtgtgag ggaacatatc gaaaagctgc acccgttcaa
     4381 tgcgataacc aggcagttcg cggatatctt tcgccatggt ttgggcgtta cagctggagt
     4441 agatgataaa acgcggtgcc atcgttgaga gataatcaca tagcggttta ccaatgccgc
     4501 gtcgcggcgg gttaaccagc accagctccg gcacctcccc ctgagcggtg gcaaactgag
     4561 tggagtccag cgcctgaaat tgcaaacgcg ttaagcccag ttcagcggct gactgctttg
     4621 cacaggcaat ggcctctggc gcaatttcga tcccggttaa ctgcatgtca ggcgtcgcgc
     4681 agtgtaaacc aaagcccccc acaccgcaga acagatccca catatgttta accggcagct
     4741 gtcgtaccca gtcgcgcgcg gtggcgtaca gctggctggc gaccaccgga ttagtctgga
     4801 agaaactttg cggacggatc cacagcggta cgtcattaaa acgctctgcc agtgcctgat
     4861 gttcggtcag gtagatctcc gtctcccctt ccataatcgc catatgtacc ggctgaatat
     4921 tgacggtaat aactttcagc tgcggtagtt gttcgtgtaa ccacggcagc gccttacgca
     4981 gttgcgccag cttggtatca gaacgcagta caaagcgcag catcatgccg ccatcgctct
     5041 ggctttcggt cagcagaatg tatttcagtt cgccacgctt acgcgccacg ttgtagggcg
     5101 ttagccccgc tcgggcgata aacggtttta gcgccgcaaa aacgggcgca aatgaggcag
     5161 gataaagcgg gcagtcacaa aggtcttccg gtgtgccgtc gcgatgcagc ataccgagca
     5221 gtggtttttc aacgctacca ctcaccacca ttttggcttt attacgaaac ccttgttccg
     5281 ggcctgacac cggcgcgcac cattcctcaa ccggaaagtc ggcgagcaga tttttaagat
     5341 cggcggtttt agcggagagt tgctctggaa tcggctgcgt tatccactga caggaacgac
     5401 agcgacccgc gtcgtaaagt gcgcactgca tacattgacc ttcacatcat caggggcgac
     5461 gattatacac attattgcaa ctggaagaac cgccgactgg tcgagggaac gaacagcagc
     5521 atcaggatga gcatatccgg cagcttctgc agcatcaggc tatggaagat ttcacgtttt
     5581 gattcaccgg gaatgctgaa cagctccgga taaccatacc ccagcgaagc tgcccacaag
     5641 taacttgcgg ccgtgatttg tgtcagcaga tatagccatc gcgcccagcg acgccctttc
     5701 accagtgaaa acgcacacca gatctcaatg aaaaccagca ccagactgct taaaaagacc
     5761 agcgttaaac tccaggtttg tacgctgcga tgaatgaact cgccaatacc gcgcacaccc
     5821 agcgtattga aaatcatcag cacgtcgagg ccacggatca taataatggc gagcgccgcc
     5881 acctgcacca gcgcaggcac attcaggcta gcatgagatg aagatgtttt cttaaaaaat
     5941 cccaacgttt cgtcttccat gaaaacaatg ccgcgacata cgcggcatta tgtagccagg
     6001 ttggcaaatt ttagtgtctt cagccacgtc ttgcacgctg gatgtcgcgt atccgctgtt
     6061 tttctgcgcg agccatcaga taccaggcga taaatccgac aattccgacc gcaccaagga
     6121 tcaatgttgc cagggcgttg atttccggat tcacccccat ccgcacgctg gaaaagacca
     6181 gcatcggtaa cgtggtggct cccggcccgg aaacaaagct ggcgatcacc agatcatcaa
     6241 gcgacaaagt aaaagccagt aaccagccag aaatgatcgc gggcatgatc atcggtagcg
     6301 taatgacgaa aaacactttc agcggcgtcg caccgagatc catcgctgct tcttctatcg
     6361 agcgatccag ttcccgcaga cgcgacgaaa taacgactgc cacataagcc gtacagaaag
     6421 tgacatgcgc cagccagatg gtaagcatac cgcggtccgc aggccagcca atggcatgag
     6481 caagcgcgac gaataataac aacagcgaca agcccgtgat gacatctggc attaccagcg
     6541 gcgcggtgat cataaaggca aagccatttg atccgcgaaa cctgccaaag cgcaccagca
     6601 ccaccgccgc aatagttccg aggatcgccg ccgccgtcgc cgcacaggcc gcaattgtca
     6661 gacttaaacc aaccgcactc atcatcgcgt catcgcgcaa taattcgcca taccagcgcg
     6721 ttgaccagcc ggcccacacc gtcaccagct tcgagctgtt aaacgaatag ataaccagca
     6781 tcagcattgg cgcgtagaga aaggtaaagc ccagcagcaa aatcacaatc cgccaaggcg
     6841 aacgaactac cggtaaatta ttcatccgtg ttctcccacg cttttttgct ggtgtttgtg
     6901 gaaccacatt atcggcacaa ttagcagcag caacatgatg atcgctaccg ccgaggccac
     6961 cggccagtcg cggttattga aaaactcttg ccatagcacg cgcccgatca tgatgctgtc
     7021 cgggccaccg agcagttccg ggatcacaaa ctcgcccaca gccgggataa acaccagcat
     7081 cgatccggca ataatcccac ctttagtcag cggcacgatc acagtaaaga acgttttcag
     7141 cggtcgtgca ccgagatcca gcgctgcttc caccagcgaa taatcaatac gaatcaacgc
     7201 ggtataaatc ggcagcacca taaacggcac gtaggcgtaa acaatgccaa tataaactgc
     7261 cagattggta tgcagaatgg tcagcggttg atcgataacc cccagccact gcagaaaatt
     7321 attcagcaca ccgttgtttt ttaatattcc catccaggcg tagacacgaa tcagaaacga
     7381 ggtccacgac ggcaggatca ccagtagtaa taagatatta cgggtcgaag gcttactgtg
     7441 cgccaccgcc cacgccagcg gatagccgat cagtaaacag caaaatgtcg aaatcgccgc
     7501 cacctgtaac gactggagat aagcatcgaa atagagcgga tcgtcggtca gttgcaggaa
     7561 attaccgaaa ttaagggtga tggacagttg cccgtcagcc cactccatca gttcggtata
     7621 aggtggaata gttcggtata aggtggaata gcgcgcgcca tctccgccag gcttatttta
     7681 aagacaatca gaaatggcag cagaaacagc aagatcaacc agatatacgg caacgcaatg
     7741 accagtttgc gcccatgctt catttgcagc tgcgacaacc acagcttaaa tccgcccggc
     7801 ggtttcgact gggcagcagg ttcaagtgta ctcatctttg ctccttaaac cgtcagcacc
     7861 acacagctgt ccacttccca gcacaaacgc acttcgtcgc cccaggtcgg taacccttta
     7921 cgatgacgat gggcgttttg tagctgggcg ctaatcatct gcccactttt cagacgaacg
     7981 tgatacaccg aaagatcgcc gagataggca atgtgtatca cctcccccac cgcgaagtta
     8041 caaccattgg cgggcggctc ttcgcaaagc atgatttttt ccgggcgcag cgctacgtgt
     8101 accggcacgt tatcgaccac tgaagcatcc gcatcgactt tcagtggatg catcagcccc
     8161 ggcgaataaa gcaccagacc atcttcctga cgctctttga gtacaccttc aaagacatta
     8221 accgagccaa taaattcggc gctatagcgg gtagtcggat gctcgtagat ctcttccggt
     8281 tcgccaatct ggacaaattt cccacgattc ataatggcga tacgccccgc catggtcatc
     8341 gcctcttcct gatcgtgagt aaccatcacg caggtcacac cgacgcgctc cagaatatcc
     8401 accacttcaa gctgcatccg gtcacgcagc tttttatcca gcgcgcccat cggctcatcg
     8461 agcagtaata gtttcgggcg tttggcaagg cttcgggcca gggccacacg ctgtcgctga
     8521 ccaccggaaa gctgatgcgg tttgcgtttg gcgaactcct gcatatgcac aaggccgagc
     8581 atctcattga cccggctggc aatttccgct ttcggtagtt tgtcctgttt caggccaaaa
     8641 gcgatgttct gttccacggt catatgcgga aacagcgcgt aagactgaaa catcatattg
     8701 atggggcgca ggtaaggcgg cacctgtgac aaatcgacgc catcgagcat aatctgcccg
     8761 gctgaaggtt gttcgaaacc tgccagcata cgcagcagcg tggacttgcc acagccggat
     8821 gcgcccagca gcgcgaagat ttcacctttg tagatggtca ggctgacatc atccaccgca
     8881 tgctgaccat cgtaggattt ggtcagattg cggatttcta atagcggggt cagcgcctta
     8941 cgggttttcg cctgggggcg agggatagcg tcattcacgg ctgctctccg gcattaaatg
     9001 tgtgcaagcg caccatgctg gtgcgaattg ccggcgggtg tggtgcgccc gcccggcatc
     9061 taaggctgcg gattattttc cgctcttcac tttggtccac gcgcgggtgc gcacacggtc
     9121 gattttcggg tcctgcactt tcagagtgaa cagcttcgca cgaacatccg caggcggaaa
     9181 aatacccggg ttatcacgga cttccgcact caccagcggc gtggcggctt tattggcgtt
     9241 agcgtagaac acatggtcag agatatgcgc caccacatca ggacgtagca ggtaattcag
     9301 gaactgatag gcttcgtctt tgtttttggc atccgcaggc atggcgaata catcaaagaa
     9361 cgccatcgcc ccttcttttg gaatcgagaa cgagacattc acgccattct tcgcttcctt
     9421 cgcgcggttt gacgcctgcc agacatcacc tgcccagccg atagcgacgc aaatatcgcc
     9481 gtttgccagg tcgttaatgt attgagatga atggaaataa cgaatgttcg ggcgcagctt
     9541 taacagcaga tcggtagccg gtccggtgta atcatccgct ttagtgctgt tgggatcttt
     9601 gccgagataa ttcagcacgg tagcaaaaac ttcttctggc gcatcgagga aagatacacc
     9661 gcagcttttc agtttttcca gattttcagg cttgaggatc aagtcccagc tatcgacggg
     9721 cgcgttttcg cccagcaccg ctttgacttt atcgacgtta tagccaatcc cggtcgtcgc
     9781 ccacatatag ggcatagcaa atttattgtc gggatcgtgt ttggcgacca acttcagcag
     9841 ttccgggtcg agattcttcc actccggcaa tttgctttta tccagcggct ggaaaactcc
     9901 cgctgtcagc tggcgttcaa gaaagcttgc tgacggcacc accaggtcaa agccggtgct
     9961 cccggccatt aatttgcctt ccagtacttc gttagagtcg aaaacatcgt agacgacttt
    10021 aataccggtt tctttttcaa agttagccac cgtgtcgggg gcgatataat cagaccagtt
    10081 ataaatgtgg agtgtttttt gttcagccgc gagcgtgccg acagagacgg ccatcagagc
    10141 acccgcaacc agacccgaaa gccatttttt atttaaggcg gtcatatctc tttccttctg
    10201 aaagttcgtt aacaaacgaa caaaagttgt cacactgaaa gatatgcaat aaacgtgcat
    10261 attttttatc ggcgcggtgt aagaaaagag aactctctcc cggccagcac tgctcgctat
    10321 ataaaagcat cgcaagaata actttagcca gggtttgaga ctatagctcg gattaaaaaa
    10381 cggtatttaa caaattttta tgcgtatttg ctctatgtga taaagccaaa tcgacacaag
    10441 agaagtgaaa taatctttaa gaaaaggtta aaaacagcag ataattttgc agcgcgcagt
    10501 cactgcacca gcgactgcgt gctcgaaagg agttatcagt ggaggaaata gttttcggta
    10561 acgttgtttt gcccttcatc gtcagtcggt aacagcagtt gatgggcatt agcttcaaga
    10621 atgaccatcg aaatctgctc ttcgctctga cgcacgaacc atgcaaactg ctcataggtt
    10681 acgccctgca taacggataa agactggcaa accaccagct ttggcagatt atcatcctgc
    10741 atatcaagaa atgctttcac ggtcagcgaa ctggcattga tggcagataa atccgccgcc
    10801 agcggcaata cggctgaagg tcggacttcc gccatggcag aaaacaggat cgtgttatcg
    10861 atcagatcga ttttggcatc aaagacaccg tcgaaattct gcatatgggg cagatgcaga
    10921 gcctgacagt tatcacattc aaaaaaactc atccccaggt catcgagcca ttgacgcagc
    10981 gtatccagac caggaacgac cagcgatgtc ataattttgt acaacctctt tcgataaaaa
    11041 gaccggcaca gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg tgattgcgac
    11101 taaccacccg ttttcaggca atattctgtc gtagcgtagc gttcgatcca gcggatcatt
    11161 ttacccgcga tgtcgatacc ggtggttttt tctattcctt ccagccccgg cgacgcattc
    11221 acttccatca ccaacggccc gcgattagca cgcagaatat ccacaccagc aacgtccagc
    11281 gccatcgttc gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat actggcgaca
    11341 cttgccgcgc cgccacgatg caaattggaa cgaaaatcgc cctctttcgc ccgccgttca
    11401 atcgcagcga ccacttcatc gccaacaacc agacagcgga tatcgcaccc ttgcgcctct
    11461 ttgatatatt cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt
    11521 tccgccgcct gacgcgtctc cgccagcacg acgccaattc cctgcgtgcc ttcaaccaac
    11581 ttgaccacca gcggcgcacc accgaccatg tcgattaaat cgctggtatc atccggcgaa
    11641 tgcgcaatgc ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg catggaacgc
    11701 aatttgtcac gcgcccgggc aatggcgacc gactcattga gcggatagct ccccagcatc
    11761 tcgaactggc gcagtgccgc cgtcccataa aaggtaatgg cggtgccaat acgcgggatc
    11821 actgcgtcaa aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt
    11881 atgttcatgt agcaagaaag cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg
    11941 gcttcacgca gccgcttaca cgaatagagc gttccatccc gggacaatat ggcaattttc
    12001 accctgcacc tctctgtcag acctggtcaa aagcccgcgt atcatacatc gacgtggcgg
    12061 ttttagcgcg tcgcccaacc ctgtttgtgc aaataatcca gaataaatgg gcggctttct
    12121 ttaatgattg ttcggcggat atgatcgctc caggtatccc ggcgattatt gctgccacgg
    12181 gtgaggtaat attccgccag ttgctcgtca tactgcgcca gtgcgtcttt atccagcggt
    12241 tgatagctgt tttcatgcac caaaatggag gccggtaaac gcggcttgag atccggatta
    12301 tccgcaggcc agccaaggca cagcccaaac agcggcagaa catgctgcgg taatttcagc
    12361 agtttcgtca ccgcttcaat attattgcgc aggccgccga tatatacgcc gcccaatccc
    12421 agcgattccg ctgcggttaa tgcattctgc gccatcattg ccgtatcaac gacaccgagc
    12481 aacagctgtt ccgccaggcc gagctgagca tccggacaga tctgtaaatg gcggttaaag
    12541 tcggcacaga acacccagaa ctccgccgct tgcgctacgt gtttttgccc gccggtcagc
    12601 gtcaccagct cttcacgtaa tgctttgtcg gtaatgcgaa taatgctact acactgcaaa
    12661 aaactggaac tggacgtcgc acgggcgctg ttaataatcg cctcacgctg cgcttcggaa
    12721 atgggttcat cagtgaaatg gcgaatggag cgatggccac aaataagttc aatggttggc
    12781 gtcattatct ttttctcttt ctgaacgtga atattgcggt ggacggttca tcagctgtgg
    12841 ggcaagacgt tttgccacct gaagaataac caccaccgca gcgggaagca tgagcaaaac
    12901 accgagaaaa atcatcagaa tctgcacttc tggccgagaa aatggctcag gcagcgacag
    12961 ggagtcgctt accgacagca gcgccaccgc cagcaacatc attccgataa attccagtat
    13021 caacacgctc ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc
    13081 ttcagtctaa aacttttcac tgtattgtgt ttaacagtta tagcttttag caattaatgc
    13141 aacaggttaa acctactttc agcgaataca ttttagcgtg atcattacag gcataaatct
    13201 ataaggagag aaataatgca aaccgttatt tttggtcgtc cgggttgccc ttactgtgtg
    13261 cgtgcaaaag atctggctga gaaattgagc aatgaacgcg atgattttca gtatcagtat
    13321 gtagatattc gtgcggaagg gatcactaaa gaagatctac aacaaaaggc aggtaaaccc
    13381 gtagaaaccg tgccgcagat ttttgtcgat cagcaacata tcggcggcta taccgatttt
    13441 gctgcatggg tgaaagaaaa tctggacgcc tgatcgtctg acaagccctc acgttgaggg
    13501 ctttactgat tttttctgtg ctgtggttta aacaaactac tgataaataa gaaacacagt
    13561 gcccccagcg cacaccagaa caccgcgctt agtaaccatg ccagctcttg ccagaatgag
    13621 cgggtcggtg aaaaaaacag ccgcataata agcattgaac agggtgccgc cagcattgcg
    13681 ccaaacagag gtttcaggac ttctctacgc tgtgaaaaga aactggcaac tgctccagga
    13741 agaatgaaaa acagcaagcc gatttcagga tgcccggcag cccgaaaagc gcctttcatg
    13801 tgcgtcgcca gaaaaaggca caccacaatg aagaggacaa aacagcagat tgcccccgcc
    13861 caacgttgtt tatgtttcac tcgttcctcc tgacactgcg tctatcgaac acatttttcg
    13921 ccagtgtggc gttcagtaag ataaagccgc ttcgcattcc atgctaatat aggccaacgc
    13981 aattcatata gccgttgata cctaatgtga ttacactagt aaaatatatt gttactttac
    14041 tatcgtttag gtgcgctgaa tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta
    14101 aattaccatt tctttcaaca gcttactagt aaacaagaag ttagcctccg tgaatataaa
    14161 cgtcgccgaa ttgttaaatg ggaattacat tctgttatta tttgtggtcc tcgcgcttgg
    14221 gctatgtctc ggaaagttac gacttggttc gatccaactg ggtaattcca ttggcgtttt
    14281 agtcgtatcg ctgttattag gccaacaaca tttcagcatt aacaccgacg cgcttaatct
    14341 tggctttatg ctgtttattt tctgcgttgg ggttgaagcc ggaccgaact ttttttccat
    14401 tttttttcgc gatggaaaaa attacctaat gttagcactg gtgatggttg gcagtgcgct
    14461 ggtgatcgcc ttagggttag gtaagctgtt tggctgggat attggcctga cggccggtat
    14521 gttagcaggc tctatgacgt cgacaccggt tctggtcggt gctggcgata cactgcgtca
    14581 ttccggcatg gaaagcaggc agctctcact ggcactggat aatctgagcc tcgggtatgc
    14641 cttaacctat ttaatcggtc tggtgagttt gattgttggt gcgcgttact tgccgaaatt
    14701 gcagcatcag gacttacaga ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac
    14761 tgatgccaac cgtaaggttt atttaccggt gatccgcgcc tatcgcgtcg gcccggagct
    14821 ggtggcctgg accgacggca aaaatctgcg tgaactgggt atttatcgac aaaccggctg
    14881 ctacattgaa cgtattcgac gtaacgggat tctggcaaat ccagacggtg atgccgtgct
    14941 acaaatgggc gatgaaatag cgttggtagg ctatcccgac gcccatgccc gactcgatcc
    15001 cagcttccgt aacggcaaag aagttttcga tcgtgacctt ctcgatatgc gtatcgtcac
    15061 tgaagaagtg gtcgttaaaa accataacgc cgttggcaaa cgtctcgcac aactgaagtt
    15121 gaccgaccac ggttgcttcc ttaaccgcgt cattcgtagc cagattgaga tgccgataga
    15181 tgacaacgtc gtgcttaaca aaggtgacgt tttacaagtc agcggcgatg cccgtcgcgt
    15241 aaaaaccatc gccgatcgca ttggctttat ctcgattcac agccaggtca ctgacttgct
    15301 ggcattctgc gccttctttg ttattgggct gatgatcggg atgatcacat tccagttcag
    15361 cacattcagt ttcggcatgg ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg
    15421 ctttatgcgt gctaaccacc cgaccttcgg ttacattccg caaggtgcat taagcatggt
    15481 gaaagagttc ggcttgatgg tgtttatggc aggcgttggt ctgagcgccg gtagcggtat
    15541 taataacggc ctgggcgcga ttggcggtca aatgttgatt gccggattga ttgtcagtct
    15601 ggtgcccgtg gttatctgtt tcttgttcgg tgcttatgta ttgcgaatga accgcgcact
    15661 gttgttcggc gcaatgatgg gcgcacgcac ctgcgcgccg gcaatggaga tcatcagtga
    15721 tacagctcgc agtaacatcc cggcgctggg ctatgcgggc acctacgcaa tcgccaacgt
    15781 tctgctgacg ctggcaggga caatcatcgt catggtatgg ccaggattag gataaaactg
    15841 aagttgccct gaaaatgaaa tttttttgca caaccgcaga acttttccgc agggcatcag
    15901 tcttaattag tgccactgct tttctttgat gtccccattt tgtggagccc atcaaccccg
    15961 ccatttcggt tcaaggttgg tgggtttttt gttatctaaa tccccaaaat ctccattctc
    16021 tcccctgtcc aaagccttat ctgactgtcc tctcttccag aaaatcattc tttttcgtac
    16081 aaagaacaaa tagttattga gaagcttatt taaaaacctg aacaaaatcg gtaatattcc
    16141 tggctttgca atacacagat tttttatatg gatattaacg agctaaaaga gtgccttcat
    16201 ctggaagtca ttggtaaaag taggaaattc acatggcgta aagtcattgt aagggcgatg
    16261 aaacatcggc gcgttcgtta cttattctgg tggcgtatcg ctaaatacgg ccatgagaaa
    16321 ggtggttact ggcggaaaat tgctggcaaa attgagcgaa aaatccttga tagttatgac
    16381 gttaaaatcc cattagtcgt tgatataggc aaagggttgg atatttcata cttaacggga
    16441 gttgttatcg gtcataacgt aaaaattggt gagaattgtt ccatcaaacc cggcgtaacc
    16501 atcggcctac gtggccattt tgacgaaatg gatatacaga ttggcaacaa tgtcaccatt
    16561 ggttgcaacg cctccattct gggcggcaaa gtctatattg gagataacgt cacgataggt
    16621 gcacatgcat tggttttgca tgatatccca gaaaactcca tttttatcaa taaaatagaa
    16681 tacgagatta tcccgaaaaa agttatagcc gaaatgtgat cgccagtgag atgattagcg
    16741 ctatcgaggg aggtgttgcg tcttatcgac ctctgtagct acccagcaac cctctcaacc
    16801 atcctcaaaa tctcctcgcg cgatagcggc ttacggtcgg tgacaaaatg cagcgtcatc
    16861 ccctcaataa acgcatcaag cgcgcgggcg gttccgggtt caaaccattg ttcgagcgtt
    16921 tgctgactgc gctgcatcca gttttgcatt accgttttta atagcggttt tcggctagcc
    16981 agcgcgtaga gctggtacat cagctccatg ttatccggcg ttgccacctg tgagctgtag
    17041 atcatatcgg tgatagcctg gcatgcgcct tgagcatcac taacatcgct aaaaaatgcc
    17101 tgatattgcc gggacatgat ctcagtaaaa ctgctgaacg cctccagcaa caactcatca
    17161 attcctgaaa aatagtaggt catcgatccc aacggtaccc cggcaagggt agcaattttg
    17221 cggtgcgtaa cagcatgtat tccgtaaagt ttcaccgcct ccagcgtggc ctggataatt
    17281 ttttctcgcc gttgcggatc gttagcgcga cgcatgtcat ttcctctgtc tgcaattgtg
    17341 tacaaatgta cacaaccttg ctaatgttgt cttccctctt cttattttga cgtgatctat
    17401 gaccgtaaat tcttcacgta atgcattgaa acgccgaacc tgggcgctgt ttatgttctt
    17461 ctttttgccg ggcctgttaa tggcgtcctg ggcaacccgt acgcctgcta tccgcgatat
    17521 tctctctgtc tcgatcgctg aaatgggcgg tgttctcttt ggtctgtcga tcggttcgat
    17581 gagcggtatt ctctgctcgg cgtggttagt gaaacgcttt gggacacgta atgtcatcct
    17641 ggtcaccatg tcctgcgcat tggtcgggat gatgatatta agtctggcac tctggctgac
    17701 atcgcccctg ctctttgccg ttggtctcgg cgtctttggg gcaagttttg gttctgcgga
    17761 agtggcgata aacgttgaag gtgccgccgt tgagcgagaa atgaataaaa cggttttgcc
    17821 gatgatgcac ggtttttata gcctgggcac gctggcaggc gctggtgtcg ggatggcact
    17881 gacggccttt ggcgttccgg caacggtgca cattttattg gcggcgctgg taggcatcgc
    17941 gcctatatat atcgccattc aggcaatccc tgacggtacg ggcaaaaatg ctgccgatgg
    18001 cacccagcat ggcgaaaaag gcgtaccttt ttatcgcgat atccagttgc tgctgattgg
    18061 tgttgtggtg ctggcgatgg cctttgccga aggttctgcc aacgactggt tacccttatt
    18121 aatggttgac ggtcacggtt ttagccccac ttccggctcg ctgatttatg ccggttttac
    18181 cctggggatg accgttggac gcttcactgg cggttggttc atcgaccgtt acagtcgcgt
    18241 tgccgtggtt cgggccagtg cgctaatggg ggcgttgggt attgggctga ttatttttgt
    18301 cgatagcgcc tgggtcgctg gggtgtctgt tgtactctgg ggactgggtg cctcgctggg
    18361 cttcccgctg accatttctg ccgccagcga taccggcccc gatgcaccaa cccgcgtcag
    18421 tgtggtagca acgaccggtt atctggcttt cctcgtcggg ccgccgctgc tgggctatct
    18481 cggcgaacat tatggattac gtagtgcaat gctggttgta ctggcgctgg ttattctcgc
    18541 ggctattgtc gcgaaagccg tcgccaaacc cgataccaaa acgcagacgg cgatggagaa
    18601 tagttgatga gcattaaatt aattgcggta gacatggatg gtactttctt aagcgatcaa
    18661 aaaacctata accgtgagcg gtttatggct cagtatcagc aaatgaaagc acaaggcatt
    18721 cgctttgtgg tcgccagcgg gaatcaatat tatcagttga tctctttctt ccctgaaatt
    18781 gctaatgaaa ttgcctttgt ggctgaaaac ggcggctggg tagtgagtga aggcaaagat
    18841 gtttttaatg gcgagctgtc gaaggatgcg tttactactg tcgtggaaca tttgctgacg
    18901 cgcccggaag tggaaattat tgcctgcgga aaaaatagcg cctatacgct caaaaaatat
    18961 gacgatgcca tgaaaacggt ggcggaaatg tattatcacc gtctggaata cgtcgataac
    19021 tttgacaact tagaagatat cttctttaag tttggcctga atctttccga tgaactgatt
    19081 ccacaagtac aaaaagcatt acatgaggcc atcggcgata ttatggtgcc ggtccacacc
    19141 ggcaacggca gcatcgatct gattatcccc ggcgtacata aagccaatgg ccttcgccaa
    19201 ctgcagaaat tatggggaat agacgacagc gaagtggtgg tctttggcga tggcggtaac
    19261 gatattgaga tgctgcgtca ggcaggcttt agttttgcaa tggaaaatgc cggcaacgcg
    19321 gtcgtcgcag cggcaaaata ccgggcaggc tccaataacc gtgaaggcgt actggatgtg
    19381 atcgataaag ttcttaaaca cgaagcgcca tttgaccaat aaaaacggca gcacgcccgg
    19441 caacacaata cctgaatgaa ggctgcataa ttcgcggaaa ttaacccgct gcattatttc
    19501 aggcgaaatg attatgaaaa attgtctact gttgggcgcg cttttaatgg gctttactgg
    19561 tgtggcgatg gcgcaaagtg tcaccgtcga tgtgccgagt ggatacaaag tggttgtggt
    19621 tcctgattca gtcagcgttc cacaagcggt cagcgtcgcg actgtgccgc aaaccgtcta
    19681 tgtggctcct gcgccagctc cggcttaccg cccgcatcct tatgttcgcc atcttgccag
    19741 cgtaggtgaa gggatggtta tcgaacacca gattgacgac catcaccatt aattcgagaa
    19801 tgcctgatcg cacaaagcaa tcaggcattt ttttaccctt cgtgagaatt tcccatctgt
    19861 ttatctttta aaaagataac catcagcgac agccacaaaa ttccgttgac aaggttgaag
    19921 agattaaaca gtccgttgcc cccgttcagc caggcatgtt tgctgatttc aataccgacg
    19981 gtaaagatca gcatttgcag cattcccatc gccgcagaaa ccgtaccttt actcatatcg
    20041 ctggcaaaca gggttaatcg caccagtccc gcattcgcca gaccaatacc gaaagcataa
    20101 atacttaacc cggcggtcat ccataaatac gcgtgcgatg agataaccgt tgccgcagca
    20161 gcgaccaaca gaccaatcat aatcggccag ccgcccataa taatcagcga acgtacggtg
    20221 cggcgcgagg tcagacgcgc taacagcaag ttacccgcaa ttaacgcccc gaaaataggc
    20281 acttgcagca agccatattc atagctgctc aactgctcgc cggtaatgat gataatcggc
    20341 gactgggcga tccacgccag caacggcagg ctaacgaatc ccagcgccaa cgcccccgcc
    20401 acaaagcggc cgttcttcag caccagctta tagtcacgac cgagttcttt cagtgacagt
    20461 ttctcgccta tacgcgtggc ggtttcaggc atagctcgtt gcagaccgaa aaaggagatc
    20521 gctgccaatg cggcaaacaa aacaaacatc ccctcccagg gcagcacatg gatccacgcc
    20581 gcgcccacca gcggaccaag tagcggagca atcagcgcca cgttcgccat cagcgcggtg
    20641 atcttgatac aaaccgcctc ttcgaaggat tcctgaattg cggcgtatcc cacagcgcca
    20701 atgaaacaga ggcttatgcc ctgcaagaag cgcaacaggg tgaattgttc aatgttttgc
    20761 gccagtaata ttgccagaca ggtgacaata aaccacacca cacccgccag catcaccgga
    20821 cggcgaccaa tacgatccga caacggcccc aggagccatt gtaaaaacat cccgcccgcc
    20881 agatacgcgg tcatcgaagt aggaacccaa tcaatgcccg cctgatattg ttccaccacg
    20941 gccaacatac cgggttgaat catatcgttg ccgatatagg ttgaaaattc gtaaagcacc
    21001 agacagagag ggaaaagtaa cgcctgacgt ccaagcctgg caccggaagc taatttattt
    21061 tgcatgcaat ttcttcgcca ataataatcg cgcaaagttt aataaaagcg cagctaacga
    21121 gaaagcgaat tttgtagctg aaaccgcggt taagcacatt cttacattat tgcgagtata
    21181 gctacgcttt ctttaagttt tatttaacct atgcccgtta caatcaccca ccgtaaacag
    21241 gccgcttgag ggaaataaga cgatgccgct ttacccagtt taacctgcac tttattctca
    21301 acgacttgcc tgtattggct cccttttaat cactttgcgt cgggaagtta tgctggaaaa
    21361 tttgaatctc tctctattct ctcttattaa cgcgacgcca gactcggctc cgtggatgat
    21421 ctcgttggcg atttttattg ctaaagattt gattaccgtg gtgccgttgc tggccgcggt
    21481 actttggttg tgggggctta cagcacaacg gcaactggtg ataaaaatcg ctatcgcgct
    21541 ggcggtcagc ctgtttgtgt cctggacgat gggacatttt tttccgcacg accgaccatt
    21601 tgtcgaaaat atcggctata acttcctgca tcatgcggcg gatgactcat tcccgagcga
    21661 tcacggtacg gtgattttca cctttgcact ggcattttta tgctggcatc gcctgtggtc
    21721 cggctcactt ttaatggtgc tggccgtcgt cattgcctgg tcgcgcgttt atcttggcgt
    21781 ccactggcca ctggatatgc tcggtggatt gctggcaggt atgattggct gccttagtgc
    21841 ccagattatc tggcaagcga tggggcataa actctatcaa cgtctgcaat cgtggtatcg
    21901 cttctgtttt gcattaccga tccgcaaagg ctgggtgcgt gactgatctc tgctaaaaag
    21961 tgtagtattg agcggctcgc ttcaataact attcagaggg attatggaaa cacgtcgcga
    22021 agagcgtatc gggcagctgc tgcaagaatt aaaacgcagc gataagttac atcttaaaga
    22081 cgccgccgcc ctgcttgggg tttcggagat gacgattcgt cgcgatttga acaaccacag
    22141 tgcgcccgtc gttttgctcg gcggctatat tgttctggaa ccgcgcagtg ccagccatta
    22201 cctgttaagc gatcaaaaat cccgcctggt ggaagaaaaa cgccgggcgg caaaactggc
    22261 tgcgacgctg gtagaacccg atcagaccct cttttttgac tgtggcacca ccacgccgtg
    22321 gattattgaa gcgattgata atgaaatacc tttcaccgcc gtttgttatt cgctaaatac
    22381 ctttctggcg ctgaaagaga aaccccattg ccgcgcgttt ctttgcggtg gtgaatttca
    22441 cgccagcaac gccattttca aacccatcga ttttcagcaa acgctgaata atttttgccc
    22501 ggatatcgct ttttattctg cggcgggtgt gcatgtcagt aaaggcgcta cctgttttaa
    22561 tcttgaagag ttgccggtaa aacactgggc catgtcgatg gcgcaaaagc atgtgctggt
    22621 tgtcgaccac agtaaatttg gcaaggtgcg tccggcgcgc atgggtgacc tgaaacgctt
    22681 tgatattgtg gtgagcgatt gttgcccgga agatgagtat gtgaagtacg cgcagacgca
    22741 gcgtattaag ttgatgtatt aatgatgtat aaccggatga cgtttcgcgc catccggtta
    22801 tcagaagatt aagagaacca gctgccgaac cactgatgga atttcatcat cacgaaatcc
    22861 cacatccgac caaagaatcc gccctcttcc acattttcca tcacgatcag cggacgctgc
    22921 tcaatggatt taccgttaag ctggaaatca atggtgccga caacctgacc ttttttcagc
    22981 ggtgcggtaa gctgcggttc cgttaacgtg tagctcgcct tcaggttttt cagctgccca
    23041 cgcggtatgg tcactgatcc cgcttcgcct gccccgagat tcacttcgct cttatcacca
    23101 aaccagacgc gctgagtcac aaaggtggca tcaggtttaa ttggcgtcac ggtttcaaag
    23161 aagcggaaac cccaggtaaa taatttctca gactcattaa aacggatacg gtcagttttc
    23221 gcccccagca ctacggagat taaacgcata tcgccctggg tagccgaagc aaccagatta
    23281 tatcccgcgc ctgccgtggt tcctgtcttc atgccatcaa cattcagatt actgctccac
    23341 agcagacggt tacggttagg ctgacgaatt ttgttgaagg tgaactcttt ctctttatga
    23401 atggcgtact cttccggcac atcgtggatc aatgctttgc ccagcaatgc catatcgcgc
    23461 gcggtactga actgccccgg cgcatccaga ccgtgcaccg tctggaaggt agtgttggtc
    23521 agacccagtt ttttggcata accattcatc aaaccaataa atgactcctg gctcccggca
    23581 acgtaatcgg ccagcgcaat acaggcgtca ttaccggact ggataatcac acctttgttc
    23641 aagtctgcca ccgaaacctg atcgcccggt ttgaggaaca ttactgatga accacgcagt
    23701 gccggatttc ccgtcgccca ggcatcttta ccgaccgtca ccatatcggt gagtttaatc
    23761 ttatcggcct taagcgcctg cccaaccaca tagctggtca tgattttagt caggctcgcg
    23821 ggatccagtt tctcatccgc attgccttct gccagcactt taccgctggc gtaatccatt
    23881 aaaatccatg cacgcgcatc cacgctcggc gcttcaacgg tttgttccgc cgcgaatgcc
    23941 gttggggcaa aaaggaataa aaatgcagaa cccgctgcaa gaccacgaag gagagaggag
    24001 tattgcgtca taaatgccac ccgagtatcc attcaaaaaa aaatacgtta tatcgccgta
    24061 tgataagttc ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag
    24121 tttttaaagt ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag
    24181 ctgttgctta aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct
    24241 gtcggaaaag gggtgagtat gattacgctg tggggtcgga ataattcaac taacgtaaaa
    24301 aaagtattgc tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt
    24361 gagtttggga taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg
    24421 ttgttgcgtg acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat
    24481 ctggcggcac agtacgggca aaaacgtctg tggatcgact caccggcacg tcgtgcggaa
    24541 gcggaaaaat ggatggactg ggcaaaccag acactcagta atgctcatcg cgggatcctg
    24601 atgggattag tcagaacacc acccgaagag cgcgatcagg ccgccattga tgccagttgc
    24661 aaagagtgcg acgccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc
    24721 tctggcgacg agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt
    24781 aacgttggcc tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact
    24841 gaacgccccg cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact
    24901 ttaagtaatt ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccggtg
    24961 cgaacatcac gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg
    25021 tcgccgttga cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt
    25081 tgctgccacc gggggaattt atcgctgtta tagaaggcca tgccgctcac ggcgggcgaa
    25141 tctttccagt aaaaaacagg ttgctcggtc cccgcgacga tctccccttt cgcttccggt
    25201 atcttaaagc ctgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct
    25261 ttttgcggga tattaatttc atcaccaccg cgcgggccat gttcattcag ccacagtgca
    25321 ttactccacg gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg
    25381 gcgcgcacac cggattcctt tataaaagga ttatcatccg ggatttcgcc caggtcggtc
    25441 agacgcacca gtttgccctg taatttatcc agatcctgcg ccgtcgagcg ctgattgttt
    25501 tcgcccagag caacaaaaag ataaccttta ccgtcgaata ccagccgccc gccaaaatgg
    25561 ttgccggtag acagttttgg catctggcga aagacggtgc ggaagtcggt cacttttgag
    25621 agatcatcac ttaagcggcc ataccccaca gcagttccgg ctttgccatc atcgccaact
    25681 tcggaataac ttaaccagat gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc
    25741 agcaggccgc cctgcccgtg cgcccaaacg tccggaactc cggaaagcgg cgcagataat
    25801 ccttttcctg cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga
    25861 ttatcgggta aaaaggccag tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca
    25921 tttaccgttg caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa
    25981 aagaattgtc gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag
    26041 gagataacaa attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc
    26101 ttagttttca ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta
    26161 agctcatcag acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat
    26221 ggtgcccact catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag
    26281 cactccactc atcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc
    26341 gattcagttt aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt
    26401 ttgccttcct cagttgcacg tatgcggcga ggtcaacgcg tgcgtcgatc aaccggttca
    26461 gcagatcgat tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt
    26521 gcaattaaat agtatggcta ttttttgagc aaaatgctgt ccaggatcaa ttcttcacgc
    26581 cgaattaaca aaatttacat ttccttcgcc tccgccagcg aatgagattg cgatacaaat
    26641 tttatccaga actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa
    26701 gctatgagca aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa
    26761 aaccttgttg attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta
    26821 ccgagctatg acgatgcgga catggtgata gtcaacacct gcggctttat tgacagcgcg
    26881 gtacaagaat cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg
    26941 accggttgtc tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa
    27001 atcaccgggc ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa
    27061 ccgaaacaca acccattcct gagcctggtg ccagaacaag gtgtcaaact gacgccgcgt
    27121 cattatgcct atctgaaaat ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt
    27181 ccgtctatgc gcggcgacct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa
    27241 cgtctggtag atgcgggcgt taaagagatt ctggtgatct cgcaggatac ttccgcctat
    27301 ggcgttgatg ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg
    27361 gtcagcctgt gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctatgtttat
    27421 ccttatccgc atgtggacga cgtcatccca ctgatggcgg aaggcaaaat cctgccgtat
    27481 ctggacattc cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt
    27541 tctgtagatc gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc
    27601 ctacgctcaa cctttattgt cggcttccct ggcgagacgg aagaagattt ccagatgcta
    27661 ctcgacttcc tgaaagaagc gcgtctggat cgcgttggct gctttaaata cagcccggtt
    27721 gaaggtgcag acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc
    27781 tggaaccgtt tcatgcagct gcagcagcag atttccgccg agcgcctgca agagaaagtg
    27841 ggccgtgaaa ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc
    27901 atggcagatg caccggaaat cgacggtgcg gtttatctca atggtgaaac caacgttaag
    27961 ccgggtgata tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt gtggggtagc
    28021 cgggtttaat taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact
    28081 gccagctaat ctgtgccctg ccgtttttct tactcttata cagccgctca tccgccagca
    28141 ttttcaatcg ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta
    28201 aattaatgct tcccacctgg aaatgaacga tttcttttag aatcgatagc aactcctcca
    28261 gcgttccacc cgtataaatc accgcaaatt cgtcaccgcc gaagcggtag actttacctt
    28321 tatctaccgg gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca
    28381 gcaaatgacc gaaggtgtcg ttaatatttt tgaatttatc gacgtcaaac aggcacaaat
    28441 aaccgccttt ttccggcagc gcttttaaat cttcgtcaaa ggattttcta cccattgctt
    28501 tggtcaggtc gtccaggaac gccgagttat acatactgta gagttttttg acatcggcag
    28561 ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt
    28621 tctccacaat accacgatta atcactctgc gtaccagata gaggcaactg gcacccgata
    28681 tcactgccac caatgcggca aagaaaataa tcaatgtaca gacccgcaaa taaacatctt
    28741 ttttatacgc aaatacgtac aatccggtcg gcaaataggc gaagtgctcc atcgcttccg
    28801 aaacggtgaa gatggcgttt ttagcattat attcatcacc ggtcaccggt ttaccgttct
    28861 taaacagaga gaacatcagg ttctcgctgt ccatcgccag cgattttgac agtaaagaaa
    28921 aacgctccag accaagcaaa aatatcagac tacgattgtg agttaccgga tagagataat
    28981 agacgaaggt tttaccgtca aaagagataa accctgtatt cttgacatgt cgatacttca
    29041 caacctgcac aatatctgca caataatttt tctcgaactc ctttacctga atacattcgt
    29101 cactggaaat cagataagga ccgacatcgt acaaataaga gggtgaaacc gagtcagcgt
    29161 aagctgtgcg taagccaacc aacagctgtt cctccgtggg atttagcgta ttgataagat
    29221 caattgccac gctacgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa
    29281 cggactgttt caggccatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa
    29341 ctgctgaaat catgataaaa atcagcagac tgactgtaag tacgaactta ttgattctgg
    29401 acatacgtaa actactcttt tactaatttt ccacttttat cccaggcgga gaatggcatg
    29461 ggtttctggt acagataccc ctgagcctga acacatccta atttgattaa ttcgcgcgca
    29521 acgtcttcgc tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccaggtcg
    29581 aaaataatgc gcacaatttt tcccgcagta ccgaaaatat cgccggtaaa ttgcgagtcc
    29641 acctttatgc tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca
    29701 aagtcatcaa tcgatatccg taccccttgt ttacgcagtt gttcaatatt gagataaaaa
    29761 tgttcattaa ttcgctcgat cacggtttca gtgatctcaa cacagaggct gttcgccttc
    29821 aggccatttt ctttcatgat ggtggtaaaa cgctcatgaa agtgcggttc gtttaactgc
    29881 cagggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa
    29941 tgtctgaatt cacccattgc cgttttaata atctgataac ccagctcatt gataaacccg
    30001 atatcttcag caatggtaat gaacttcagc ggtgaaatga tcccccgctc cgcagacacc
    30061 caacggcaca acgcctccgc gcccagaatg gcgcgggtgt taatatcaac gatcggttga
    30121 tagacgagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata
    30181 ttctcttcta tttcgttgag tttcgcgtca tcacagaaaa taaactctga gaacatatgg
    30241 tcacggatac tggaaatcgc cagtttcagt ttttctgcgt aactccaggc gttttctccc
    30301 tgcaaaggtt cacaaatggc agacttatgg atgcgcagat tgcggtagcc aaagctgctg
    30361 tcaaaatcgt ttaccatcgc tattttacgg gtaaatgtct gtacattttc ccggcacaga
    30421 agtaaataga gatcggcatt gaatgtcacg atatcgcagc attgtgcata tttctcagca
    30481 attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc
    30541 acggcttcca gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac
    30601 tcaataatcg ccatattttt aataaaacca ctttcctgat cgtaagagat cagtgagagc
    30661 aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaatt tttgatgctg
    30721 ataatctctt tcgcatcgcc ttcgcgaatt ttcagagcct cgatttgatc cgttgagtca
    30781 ggcaaaaacg ccagcgtatt aaccaactct gtcatttcag aaaattgccg acggaacaca
    30841 cgagtggtca ttaaataaac caccagcaat acgataataa tgaacgcaaa cggcagataa
    30901 aaaagtttat ctgatgcgtt caataaatta ccgcccggat aataaaaata gatttcgaat
    30961 aatgcgttgt ttgacaccgc tgatgttttt tcgatatatt cagtatcgac agaatcaata
    31021 at
//
LOCUS       DAZUGK010000034        29863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZUGK010000034 DAZUGK010000000
VERSION     DAZUGK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.034"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            104..1687
                     /gene="yegH"
                     /locus_tag="ACHY5W_001389"
     CDS             104..1687
                     /gene="yegH"
                     /locus_tag="ACHY5W_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS4446317.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1980..3833)
                     /gene="asmA"
                     /locus_tag="ACHY5W_001390"
     CDS             complement(1980..3833)
                     /gene="asmA"
                     /locus_tag="ACHY5W_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFS4446318.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQVSNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3855..4436)
                     /gene="dcd"
                     /locus_tag="ACHY5W_001391"
     CDS             complement(3855..4436)
                     /gene="dcd"
                     /locus_tag="ACHY5W_001391"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFS4446319.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4528..5169)
                     /gene="udk"
                     /locus_tag="ACHY5W_001392"
     CDS             complement(4528..5169)
                     /gene="udk"
                     /locus_tag="ACHY5W_001392"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4446320.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5487..8804
                     /gene="dgcE"
                     /locus_tag="ACHY5W_001393"
     CDS             5487..8804
                     /gene="dgcE"
                     /locus_tag="ACHY5W_001393"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS4446321.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYCITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISISLPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8905..9753)
                     /gene="alkA"
                     /locus_tag="ACHY5W_001394"
     CDS             complement(8905..9753)
                     /gene="alkA"
                     /locus_tag="ACHY5W_001394"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFS4446322.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAVPDIARHTLHINLSVGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGSW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9887..11239
                     /gene="yegD"
                     /locus_tag="ACHY5W_001395"
     CDS             9887..11239
                     /gene="yegD"
                     /locus_tag="ACHY5W_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4446323.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLCRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11252..13192)
                     /gene="yegI"
                     /locus_tag="ACHY5W_001396"
     CDS             complement(11252..13192)
                     /gene="yegI"
                     /locus_tag="ACHY5W_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFS4446324.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13189..13950)
                     /gene="pphC"
                     /locus_tag="ACHY5W_001397"
     CDS             complement(13189..13950)
                     /gene="pphC"
                     /locus_tag="ACHY5W_001397"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFS4446325.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELLLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13947..14606)
                     /gene="yegL"
                     /locus_tag="ACHY5W_001398"
     CDS             complement(13947..14606)
                     /gene="yegL"
                     /locus_tag="ACHY5W_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS4446326.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGASITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14827..14886)
                     /gene="ibsA"
                     /locus_tag="ACHY5W_001399"
     CDS             complement(14827..14886)
                     /gene="ibsA"
                     /locus_tag="ACHY5W_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFS4446327.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15160..15216)
                     /locus_tag="ACHY5W_001400"
     CDS             complement(15160..15216)
                     /locus_tag="ACHY5W_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4446328.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15488..15544)
                     /locus_tag="ACHY5W_001401"
     CDS             complement(15488..15544)
                     /locus_tag="ACHY5W_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4446329.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15816..15872)
                     /locus_tag="ACHY5W_001402"
     CDS             complement(15816..15872)
                     /locus_tag="ACHY5W_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS4446330.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16151..17398
                     /gene="mdtA"
                     /locus_tag="ACHY5W_001403"
     CDS             16151..17398
                     /gene="mdtA"
                     /locus_tag="ACHY5W_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFS4446331.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17398..20520
                     /gene="mdtB"
                     /locus_tag="ACHY5W_001404"
     CDS             17398..20520
                     /gene="mdtB"
                     /locus_tag="ACHY5W_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFS4446332.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVSLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20521..23598
                     /gene="mdtC"
                     /locus_tag="ACHY5W_001405"
     CDS             20521..23598
                     /gene="mdtC"
                     /locus_tag="ACHY5W_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFS4446333.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23599..25014
                     /gene="mdtD"
                     /locus_tag="ACHY5W_001406"
     CDS             23599..25014
                     /gene="mdtD"
                     /locus_tag="ACHY5W_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446334.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25011..26414
                     /gene="baeS"
                     /locus_tag="ACHY5W_001407"
     CDS             25011..26414
                     /gene="baeS"
                     /locus_tag="ACHY5W_001407"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFS4446335.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26411..27133
                     /gene="baeR"
                     /locus_tag="ACHY5W_001408"
     CDS             26411..27133
                     /gene="baeR"
                     /locus_tag="ACHY5W_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFS4446336.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27324..27656
                     /gene="yegP"
                     /locus_tag="ACHY5W_001409"
     CDS             27324..27656
                     /gene="yegP"
                     /locus_tag="ACHY5W_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFS4446337.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27803..29164
                     /gene="yegQ"
                     /locus_tag="ACHY5W_001410"
     CDS             27803..29164
                     /gene="yegQ"
                     /locus_tag="ACHY5W_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFS4446338.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29495..29812)
                     /gene="yegR"
                     /locus_tag="ACHY5W_001411"
     CDS             complement(29495..29812)
                     /gene="yegR"
                     /locus_tag="ACHY5W_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFS4446339.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
BASE COUNT         6834 a         8008 c         7901 g         7120 t
ORIGIN      
        1 aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta tccatgatcg
       61 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
      121 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
      181 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc
      241 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
      301 ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
      361 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
      421 cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg cgaagttctg
      481 gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact cagtgattac
      541 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
      601 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgacgatcgt
      661 tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
      721 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
      781 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacgctgcg
      841 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag atgcggaact
      901 ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct ttaatccgca
      961 ggaacggcgg atgattgagc gggtactaaa tcttaaccag cgtaccgtca gcagcattat
     1021 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagacg agatccgcca
     1081 gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg acgcagaaga
     1141 tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg gcgaaccgct
     1201 caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc cgttgttacc
     1261 tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg atgagtttgg
     1321 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg gtaacttacc
     1381 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg acggttcctg
     1441 gacggcgaat ggtcatatgc cgctggagga tctggtgcaa tatgtgccgc tgccgctgga
     1501 tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gagtatttgc agcgtattcc
     1561 aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc aggtagaaag
     1621 ccatcgcgtg cagaaggtgc agattatacc gctgcgtaag gatggcgaga tggagtacga
     1681 ggtgtgatgg tatcggtcat tttacccgat gctgcgtaac gccttataac ttcagcaatg
     1741 aagcgttgtc tgtggtacat ggacatcaca gcgggggtgt gggcccgggt tgccagggtg
     1801 gtggcgattg agccaccctg gcacgttcac cggcttgagc gttccatagt aacgaggaac
     1861 atcaagtgaa cggaacagcc ttcgatctta catattcaga atataatgat gccgccacga
     1921 acgcctgatg cgacgcttta cgcgtcttat caggcctaca ggactggtca cctggtaacc
     1981 tacatcttct caagcaactt cttcacatct ttgccattac gggaatcttt attccgctcg
     2041 gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag taactgatcc
     2101 acttgcaggc tgtaattgag ttgctgccag ttgccataaa cccgcagcgg tactggcgtt
     2161 tctttcagga aatcaatcag tttgctttcc ccgttccagc cacccacgac ccgaatatca
     2221 aactgggtgt cgcaggtttg atctgccaga ttcaacatgc cttcccctgt cagcgccagc
     2281 acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg
     2341 gtggtaaagc ggtcaagccg cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg
     2401 ccattacgct ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg
     2461 tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg
     2521 tcagcaccgg agaaatcacc agccagtgac atttttccgg tcaacgaaat cggatagtta
     2581 aacgccttca gaatggtacc aatctcaacg ttttccagcc gtggctggaa gtttatccgc
     2641 ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc gttaagcttg
     2701 ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct
     2761 gtaaaattca ttccgcgcca gcgcacgtta ctgacctgca acaaaatatc agccgtaaag
     2821 ccttgcagtc cctgataggc cggttcatca atacgcgaag aaataaccgg gcgcggcaac
     2881 gttgattggc tctgcccctg ctgcgcggca ccgttttcac cattcgctgt ttcattaagc
     2941 gggatgaggt tgtcaagatt cagttgcggg aattgcagcc tcagctgcca ttccggtttc
     3001 tctgtcagcg taacctgtgc ttgcccgctc agcgtactgt cattggcggt caagctgatt
     3061 tggttaaatg aaaggcgttt atgtgactcc tgccactggg cctggaaact cccctgcccc
     3121 tgaatacctt gttttggtaa atcggcaccc tgcaactgcc agttaatttg ttcaatagcc
     3181 gccgttaaat catgcggata atcagaagca tctaccgtac cgttaaggga tatcgtgaga
     3241 tcgcgctgat cgcgattaac ccgcccggaa aactcaaatg agccacgatg ttggggatct
     3301 tgttccattt gcaggcggat attgcggatt gtcacctgct cgtcatcttc atgctggaac
     3361 accagaacgc tgtccgccac cttaagactg gatatatcaa acgaccatcc gcgatcgtct
     3421 gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct
     3481 tccgtctgcg gcgtcagttg gatcactgcc ccttttagca tcacctgctt aacgctcagt
     3541 tgatgactca gtagtggtaa aagcgccacg tccagacgca tgttgtcggc gcgaaccagt
     3601 ggctggcttg cgccctgggc ggtgagagac attcgcccgg agaggatact aagctgcggc
     3661 cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg tgcagcaact
     3721 tgcttgacca tatagtcgcg gaaatcattc ggattcacca gcaacactaa cgcagataac
     3781 ccggccacca gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct catggtatcc
     3841 tcaatgggcc tgaattagtc tttatcgatt cggctggcta ccgcgccctg ctggttgcga
     3901 tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag
     3961 ctcagcgcac caattaacat gcccggacgc agcgccagcg gcagcttacc ggagttgtag
     4021 aactccagca caatgcaacc agaccagccc ggatcgatgc ggtgcgcggt gacgtgcacc
     4081 atcagcccca gacgcgccag tgaggaacgc ccgtccagcc agcccaccag atcggctggc
     4141 agcgtcaccg actccagcgt caccgccagc gccagctctc ctgggtgaag atagaacgcc
     4201 tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt caagcgcggc gctcacttca
     4261 tctttgggac cgctcagatc gataaacgct gccgtgtgac cacggaaggt acgaaattta
     4321 ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg
     4381 atcgacaaac ggccttcatc aagccaggct tcaatatctc ggtcacacag acgcatggca
     4441 ttttctcctt tcgcgcatca ctcccttaac gccaattacg tcaagggcat accaggttat
     4501 cactgaacgg tacacaattt atcaagctta ttcaaagaac tgacttattt tcgctttcaa
     4561 tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg catattgttt
     4621 agaaggctca atgaattgca ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac
     4681 tgaatccatt gaacgcccac gctcgttaac gtcacgcttg atgcggcgca tcaggcagat
     4741 atccagcggg gtatcaacga aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt
     4801 cagcaacaaa atgccttcga gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt
     4861 catacgcgta tgttcaacat agctgtaaac cggcaggtca attgccgagc cgcgtttcaa
     4921 cgcttgtaaa tgctcaagca gcagactgtg atccatcgcg ctgggatggt cgtagttggt
     4981 cttaacgcgt tcttccatcg acagatggct ttgatctttg taatagcagt cttcgggaat
     5041 tacgccgatg tgttcatcac cgacttgctc acgcaattca cgataaaggg tactggcaat
     5101 aagactcttg ccggaagccg atgcgccagc gataccgata atgacgcact gatgagactg
     5161 atcagtcata tatttagcga cctgattaac ctggatgtta ggaagggggc gacgaagcgc
     5221 caaacgcggc aattataggg atttcatccg tctgatacca gtcgaatagc gttgccgcgc
     5281 gctcagagtt aattgttgac aaagaattcc cgggggcaaa ttacgttgat cagttttatg
     5341 taaggtaaaa aatgttatac gcagttgcgc aaattatccg cctttacgtc actttatgag
     5401 caattcgcat ataaaatgta aaacttttgt actagcataa acacagaaac gaatactggc
     5461 gaccaggtct tgcggataaa gcggtaatga gcaaacaatc acagcatgta ttaattgccc
     5521 tgccccaccc gctgcttcac ctggtcagtt taggtttagt ctcgtttatc tttacccttt
     5581 tctcgcttga gctttcgcag tttggcaccc aactcgcccc actgtggttc ccgacgtcca
     5641 tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct
     5701 gttcgctggg aaatatcgcc gcatccatcc tgcttttttc caccagctcg ctgaacatga
     5761 cctggacgac catcaatatt gttgaagccg tggtcggggc agtgctgcta cgtaaattgc
     5821 tgccgtggta taaccccttg caaaatctgg ctgactggct gcgtctggca ctcggcagcg
     5881 ccattgttcc gcctctgttg gggggtgttc tggttgtcct gctgacgccc ggagacgatc
     5941 ctctcagggc atttttgata tgggtactgt cagaatccat cggcgctctg gcactggtgc
     6001 cgctgggatt gttatttaaa ccacactatc tgctgcgcca tcgcaaccca cggttgcttt
     6061 ttgagtcgct gctcacgtta gccatcacac tgacgttaag ctggctttcg atgctgtatc
     6121 tgccgtggcc ttttactttc attattgtgc tgttgatgtg gagcgccgtg cgtctgccac
     6181 gaatggaagc ctttttgatc ttccttacca cggtgatgat ggtgtcgctg atgatggccg
     6241 cggatccctc cctgcttgct acgccgcgta cgtacctgat gagccatatg ccgtggctac
     6301 cgtttttgct gatcctgctg cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg
     6361 cggaacgcaa acacatttcc gaaagcgaaa cccactttcg gaacgcgatg gaatattccg
     6421 ctatcggtat ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac aaagcgctct
     6481 gccagtttct cgggtacagt caggaagagc tgcgcggact cacctttcag caactgacct
     6541 ggccggagga tctcaataaa gatctccaac aggttgaaaa gctgataagc ggtgaaataa
     6601 acacctattc aatggaaaaa cgctactaca accgcaatgg cgatgttgtc tgggcgttgc
     6661 ttgccgtctc actggtgcgc cacacggatg gcacgccgct ctattttatc gctcagattg
     6721 aagacattaa cgagctaaaa cgcaccgaac aggtcaatca gcaactgatg gagcgcatca
     6781 cgctggctaa cgaagcgggc gggattggca tctgggagtg ggagttgaag ccgaatattt
     6841 ttagctggga taagcggatg ttcgagctgt atgaaattcc tccgcatatc aaaccgaact
     6901 ggcaggtgtg gtacgagtgt gtattgccgg aagatcgcca acacgccgaa aaagtgattc
     6961 gtgattcgtt gcaatcacgc tcgcccttta agctggaatt tcgcattgcc gtgaaagatg
     7021 gcatccgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac
     7081 gcctgctcgg cattaatatg gatatgactg aggttaaaca gcttaacgag gcattgtttc
     7141 aggaaaaaga gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg
     7201 atatggcgat gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc
     7261 aggaagaagc gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg
     7321 gaccattaat ggagaacatt tacagtgccg acacctcacg ttccgcgatc gagcaagatg
     7381 tggtgttgca ctgtcggagc ggcggcagtt acgacgtgca ttactgtatt acgccgttaa
     7441 gtactctgga cggcagcaat attggttcgg ttctggtgat tcaggacgtt accgaatcac
     7501 gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca
     7561 accgcgccag ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacacatc
     7621 agcgacatgc cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg
     7681 ggcatgcggc gggtgacgct ttgctgcgcg aactggcgtc gttaatgctg agtatgctgc
     7741 gttccagcga cgtgctggcg cgactcggtg gggatgaatt tggtctgctg ctaccagact
     7801 gtaatgttga aagcgcgcgt tttatcgcta cacgcattat cagtgccgtg aatgactatc
     7861 actttatatg ggaaggccgt gtgcatcggg taggtgccag tgccgggatt accttgattg
     7921 atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct
     7981 ccaaaaatgg tggccggggc cgggtgacgg tttacgaacc gcagcaagct gccgcacata
     8041 gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga
     8101 tgatgctcgc ccacggtgtc gcttcgccac ggatcccgga agcgcgtaat ttgtggctga
     8161 tttcacttaa gctctggagt tgcgaaggcg agattattga tgaacaaaca tttcgtcgta
     8221 gcttcagcga tccggcgctt agccatgctc ttgaccgccg ggtattccac gaatttttcc
     8281 agcaggccgc aaaagcggtt gccagtaaag gcataagcat ctccctcccc ctttccgttg
     8341 ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc
     8401 taccaccacg gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa
     8461 gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc
     8521 tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt
     8581 tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc
     8641 acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg
     8701 atacgctttc tggcatcggc gtcgatctga tttatggtga ggtgattgcc gatgcccaac
     8761 cgctggattt gctggtgaat agcagttatt tcgcgattaa ctgagggagc gtttgcccat
     8821 tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa tcgctcattc cgcaaggcgc
     8881 tcgcaccgct tccggcgacc aacgtcatgc ttcgtctggc tgccagcctt ccgtatacca
     8941 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
     9001 cgccggtgtc attcccggaa atcgctgttt aatcagataa tcatccggca ggaaaacatc
     9061 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagctcccga tacccggaaa
     9121 agtttgcagc gttttcatcg cctgctccac atcgcccggt attgtcattg gtaaggtgcc
     9181 ctccagcgcc gcatttgcca gatgaatcag cgcctctgcc cgtttcaacg gcatacctaa
     9241 cgcttttaat gcctgcgggt ctgctgctgc cagccgctga ggcgtgggga agcagatata
     9301 ctccggaaaa tcatccagcc gttcgccata aagctgtgcc actctggcgg tcaattttgc
     9361 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acgccctgct caaaagcatc
     9421 aacactgccg ggtaaacgca atccgggccg cgccgcgcct aacttgccca acgcaccgtt
     9481 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactcagc
     9541 ggcaacgggt tctaaaccta cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
     9601 aacagcagtc accacgccgc gatattcgcc caccgccaga ctacgggcat aataactgtc
     9661 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
     9721 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttattcat ccgctttcag
     9781 cataaacgtt attcagacgc tacgctttgc tttcatattc cggttgtcgc gacggcaaca
     9841 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
     9901 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
     9961 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaagc
    10021 agtaagtgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    10081 gctgttatgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    10141 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    10201 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    10261 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    10321 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    10381 cgatgaagca aacgcccagg cgcaagggat tctggaacgc gcggcgaagc gtgccggatt
    10441 cagggacgtg gtattccagt acgagccggt cgcggctggg ctggattacg aagccacctt
    10501 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    10561 gctgctgatg ggaccgcagt ggcgttcgcg tctcgatcgt gaagccagcc tgctgggtca
    10621 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    10681 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    10741 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    10801 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    10861 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    10921 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    10981 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tgacgcggat
    11041 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    11101 gaccggcggt agcgcccgtt ctccgctgat taaaaaagcg ctggcagaac agttgccggg
    11161 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    11221 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    11281 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    11341 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    11401 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    11461 tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc gcctgggtca
    11521 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    11581 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    11641 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    11701 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    11761 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    11821 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    11881 ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgccagc acaggaacca
    11941 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    12001 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    12061 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    12121 ccatcgccca gactttcgcc agcacaaaat tcccgccggt ggtaatgacc tcttcgccga
    12181 gatcaataaa atagataacg ccttgattat ccagcgtaca ccacgggcag tcggtcaaat
    12241 gagcgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    12301 ccagtgctgc tacccacgct ttagccgtcg gacgcccggt tgctacacca ctttccgtaa
    12361 acgcctgctg aaacatggct tcaacatcgc ccggtaacat cgacagcgga atcgatcgtg
    12421 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggcaa
    12481 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    12541 gcccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattcgcgg
    12601 tacgttcaaa accgacaaat gatggcaagg tttgtagctc tggcggcgta aaatgcgaca
    12661 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcactatcga
    12721 tcaacaccac tttgctgtcg cgacctacca taaagctgtt ctggtttacg tcccccacga
    12781 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    12841 gaaaatccca cgcacaatga gggtaactct ggcgacgatg tgccgggcta tagatcatat
    12901 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgcc
    12961 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caactgcgcg tcagctgtcg
    13021 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    13081 cgacgctatc ggcaaactcc tcgatatcat aaaccgcacc ttcaccgcct ttgcccagtt
    13141 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    13201 cagcgccagt gccaacgttt tatcatcatc ggtacgctcg tttaccgccg ggctcgacaa
    13261 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg ccgccagtcc
    13321 attgaaaaac ggggtaaaga aaggcacatg cggagaatta tccagcatat ttaacgccag
    13381 tcgctgaata ccatccgtaa atgctgcaac tttatgcgca cgctcagtgc tggtgaaagt
    13441 ttccagtctg gaaacagcat cttcatcgct aatgaaatgc gtcatattgg catattcacc
    13501 caccatcggc gtgagcagca gttccagacc acgaccaaaa tccaccacta caccgccgtc
    13561 gccaatttgc ataatcaatg tgccgttagc cgatgaaatc aatcccagaa acgtgcaggc
    13621 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatggt
    13681 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    13741 caccataaac gccatcgctt cattgacggc gagcttcgcg ccttctcctc cctgcgaaac
    13801 gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat ttagccaggc
    13861 aacctgcatc tggcaggcat cctgacaagg taaatcagcg ctaatgtgcg acgttccaac
    13921 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    13981 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gtgaactgga taaccagcta
    14041 aacagctcgc ggaattgtaa tccctgcaga gacaaaggct gacggacgct gatttgggcc
    14101 agcgtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    14161 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    14221 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc acgtttccgc
    14281 tcctctacca tatccagggc tttggtaatg gaggcaccca ttggtgtatc gccctgggca
    14341 aacagaatgg gcgggaaaaa attagcggcg ctggtaaagg gttgttccac atgcaccggg
    14401 ccgaacgtca caataccaag ttccactctt tttaaggcca gcgggtcggc aagcagctca
    14461 tcgcgaaagg taaccaatcc ggcgttaagt tcattaatgg gtctaccgtt catcgaacca
    14521 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    14581 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta ttgaggtccg
    14641 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    14701 cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    14761 aagggcggag tgacataatt tcaggagtga gggttaggga gaagtttccc cctccccctg
    14821 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    14881 catcataacc ctttccttat ttaaaagccc tcttctccgg gagagggttc cccgttttag
    14941 cgtcccgctg aaatcgtcgg cttacctcct ttcgccctga atgcagtcta tcgctgactt
    15001 gcggataaaa tagtttcaag tgtgattatt gcgtaacgac tcgcaaagaa tatttgctgg
    15061 caggatcgca gactgcaaag cctgcagatt gacaatctta tcgtgaaggc atactttcag
    15121 gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagcgg ggaagcttat
    15181 gactaagagc accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc
    15241 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcttcggct tacctccttt
    15301 cgccctgtat gcagtctatc gctgacttgc ggataaaata gtttcaagtg tgattattgc
    15361 gtaacgcctc gcaaagaata tttgctggca ggatcgcaga ctgcaaagcc tgcggattga
    15421 caatctgacc gtgaaggcat actttcagga gtgagggttg agcggggttt cccccgccct
    15481 ggtagtctta gtaagccggg aagcttatga ctaagagcac cacgatgatg atccacttca
    15541 tcatgaccct ttccttattt atggcccctt cctcgggagg ggctttcccg tttcagcgtc
    15601 ccgctgaaat cgtcggctta cctcctttcg ccctgaattc agtctatcgc taacgcgtga
    15661 acaaaatagt ttcctctgtg attattgcgt aacgtctcgc aaagaatatt tgctggcagg
    15721 atcgcagact gcaaagcctg cggattgaca ttctgaacgt gaaggcatac tttcaggagt
    15781 gagggtagag cggggtttcc cccgccctgg tagtcttagt atgctgggta gctaatcact
    15841 aagagcacca cgatgatgag tatcttcatc atgacccttt ccttatttat ggccccttcc
    15901 tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc
    15961 atgcaagcag tctatcgcta acgcgtagat aaaatagttt cctgtgttat tactggatgc
    16021 gtgctcgcaa atgtgcccgc cattcagacg attcccgata gtgtttcata attcctccat
    16081 ttttctccct tattggctgg ctacactagt atcattccgc gaaacgtttc aggaagagaa
    16141 actcttaacg atgaaaggca gttataaatc ccgttgggta atcgtaatcg tggtggttat
    16201 cgccgccatc gccgcattct ggttctggca aggccgcaat gactcccaga gtgcagcccc
    16261 aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg
    16321 cccattagcc ccggttcagg cggcgaccgc cgtagaacag gcagttccgc gttacctcac
    16381 cgggcttggc accattaccg ctgctaacac cgttacggtg cgcagccgcg tagatggtca
    16441 gttgatggcg ttacatttcc aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga
    16501 aattgacccc agccagttca aagttgcatt agcacaagcc cagggccaac tggcaaaaga
    16561 taaagccacg cttgccaacg cccgccgtga cctggcgcgt tatcaacaac tggtaaaaac
    16621 caatctcgta tctcgtcagg aactggatgc ccaacaagcg ctggtcagtg aaaccgaagg
    16681 caccattaag gctgatgaag caagcgtcgc cagcgcacag ctgcaactcg actggagccg
    16741 catcaccgca ccagtcgatg gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat
    16801 ctccagtggt gataccaccg gaattgtggt gatcacccag acgcatccta tcgatttgct
    16861 ctttaccctg ccggaaagcg atatcgctac cgttgtgcag gcgcaaaaag ccggaaaacc
    16921 gctggtggta gaagcctggg atcgcaccaa ctcgaagaaa ttaagtgaag gcacgctgtt
    16981 aagtctcgat aaccaaatcg atgccactac cggtacgatt aaagtgaaag cacgctttaa
    17041 taatcaggat gatgcgctgt ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac
    17101 cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa atgggcaatg aaggccattt
    17161 tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat ctggtgacgc cgggcattca
    17221 ggacagtcag aaagtggtga tccgcgcagg tatttctgcg ggcgatcgcg tggtgacaga
    17281 cggcattgat cgcctgaccg aaggggcgaa agtggaagtg gtggaagccc agagcgccac
    17341 cactccggaa gagaaagcca ccagccgcga atacgcgaaa aaaggagcac gctcctgatg
    17401 caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtg
    17461 gccaccacgc tgctgatggt ggcgatctta ctcgccggga ttatcggtta tcgcgccctg
    17521 cccgtttcgg cgctgccgga agtggactat ccgaccattc aggtggtcac gctctaccca
    17581 ggtgcgagcc cggatgtcat gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg
    17641 cagatgtctg gtctgaaaca gatgtcgtcg caaagttccg gcggtgcgtc agttatcact
    17701 ttgcagttcc agctaacatt accgctggat gtcgccgagc aggaagtaca ggccgcgatt
    17761 aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc cgccggttta cagcaaagtg
    17821 aacccggcag atccgccgat catgacgctc gccgtcacct caacagccat gccgatgacg
    17881 caagtggaag atatggtgga aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc
    17941 ggcctggtgt cgctttccgg cggccagcgt ccggctgttc gcgtcaaact taacgctcag
    18001 gcgattgccg ccctcggcct gaccagcgaa accgtgcgca ccgccattac cggcgctaac
    18061 gttaactcgg caaaaggtag cctcgacggc ccttcccgcg cggttacgct ttccgcgaac
    18121 gaccagatgc aatccgccga agagtatcgc cagttgatta tcgcctacca gaacggcgcg
    18181 ccgattcgtc tgggcgatgt cgcaactgta gagcaaggtg cagaaaacag ctggctcggc
    18241 gcatgggcga acaaagaaca ggccattgtg atgaatgttc agcgccagcc cggtgctaac
    18301 attatctcca ccgccgacag catccggcag atgctgccac agctcaccga gagtttgccg
    18361 aaatcggtga aggtgacggt actttctgac cgtaccacca atatccgcgc atctgtcgat
    18421 gatactcagt ttgaattgat gatggctatc gcgctggtag tcatgattat ctacctgttt
    18481 ttgcgcaata ttccggcgac catcattccc ggtgttgccg tgccgctgtc gttaatcggc
    18541 acttttgcgg ttatggtgtt tctcgatttt tcaatcaata acctgacact gatggcgtta
    18601 actatcgcca ccggattcgt ggtcgatgac gccatcgtgg tgatcgaaaa catttcccgc
    18661 tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca agggcgcagg tgaaatcggc
    18721 tttaccatta tctcgctgac cttctcactg attgcggtgt tgatcccact gctgtttatg
    18781 ggcgatatcg tcgggcgact gttccgcgaa tttgctatta ccctggcggt agcgattttg
    18841 atctcagcgg tggtgtcgct gaccctgaca ccgatgatgt gcgcgcggat gctcagccag
    18901 gagtcgttgc gtaaacagaa ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata
    18961 atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc atccgtggct gaccttaagc
    19021 gtggcactca gcacgctgct gcttagcgtg ctgctgtggg tgttcattcc gaaaggtttc
    19081 ttcccggtac aggacaatgg cattattcag ggcactttgc aggcaccgca atccagctcc
    19141 tttgccaata tggcccagcg acaacgccag gtcgcggacg tgattttgca ggatccggca
    19201 gtgcaaagcc tgacctcatt tgttggcgtt gatggcacta acccgtcgct gaacagtgca
    19261 cgtttacaaa tcaacctcaa accgttggat gaacgtgatg atcgggtgca aaaagtcatc
    19321 gcccgtctgc aaacggcggt agataaagtg ccgggcgtcg atctcttcct gcaaccaacg
    19381 caggatctga ctattgatac tcaggtcagc cgcacccagt accagtttac cttgcaggcc
    19441 acgtcactgg atgcgctcag tacctgggtg ccacagttga tggaaaaact ccagcaactg
    19501 ccacagcttt ctgatgtctc cagcgactgg caggacaaag ggctggtggc gtatgtcaat
    19561 gttgatcgcg acagcgccag ccgtctgggg atcagcatgg cggatgtcga taacgccctg
    19621 tacaacgcgt ttggtcagcg gctgatttcc actatttata ctcaggccaa ccagtatcgc
    19681 gtggtgctgg agcacaacac cgaaaatacc ccaggcctcg cggcgctgga taccattcgc
    19741 ctgaccagca gcgacggcgg cgtggtgccg ctaagctcaa ttgccaaaat tgagcagcgt
    19801 tttgcgccgc tctccatcaa ccatctggat cagttcccgg taacgaccat ctcctttaac
    19861 gtgccggata actattcgct gggcgatgcg gtgcaggcga ttatggacac cgaaaagacg
    19921 ctgaatctgc cggtggatat caccacgcag ttccagggca gcaccctcgc cttccagtcg
    19981 gcgctgggca gcactgtctg gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc
    20041 ggcattctgt acgagagctt tattcacccg atcaccattc tctcgacgct acccaccgca
    20101 ggggttggcg cactgctggc gttgctgatt gctggtagcg aactggatgt gattgcgatt
    20161 atcggcatta ttttgctgat cggtatcgtg aagaagaacg ccatcatgat gatcgacttc
    20221 gcgctggctg ccgagcgcga gcaaggcatg tcgccgcgcg aggcaatcta ccaggcttgt
    20281 ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg ctctgcttgg cgcgctgccg
    20341 ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc
    20401 ggcggcctga ttgtcagcca ggtgctgacg ctgttcacca cgccggtgat ttatctgctg
    20461 ttcgaccgcc tggcattgtg gaccaaaagc cgctttgccc gtcatgaaga ggaggcgtaa
    20521 gtgaagtttt ttgccctctt catttaccgt ccggtggcga cgattttact gtcggttgcc
    20581 attaccctgt gcggcatact gggcttccgt atgctgccgg tcgccccgct gccgcaggtc
    20641 gattttccgg tgattatggt cagcgcctcg ctgcccggtg cgtcaccaga aacaatggcg
    20701 tcttccgttg ccacgccgct ggagcgctca cttgggcgca ttgccggagt cagtgaaatg
    20761 acctccagca gttcgctcgg cagcacgcgt attattttgc agtttgattt tgaccgggat
    20821 atcaacggcg cagcgcgtga tgtgcaggcg gcgatcaacg ctgcacaaag tttgctgccc
    20881 agtgggatgc ccagccgccc gacctatcgc aaagcgaacc cgtcggatgc gccaattatg
    20941 atcctcacgc tgacgtccga tacttattcg cagggtgaac tgtacgattt cgcctcgacg
    21001 cagctggctc cgacgatttc gcaaatcgac ggtgttggtg atgtcgatgt cggaggcagc
    21061 tcactgcccg ccgtacgcgt cgggctgaat ccgcaggcgc tgtttaatca gggcgtgtcg
    21121 ctggacgacg tacgcaccgc cgtcagcaat gccaacgtgc gtaaaccgca gggcgcgctg
    21181 gaagatggca ctcaccgctg gcagatccag accaatgatg agctaaaaac cgccgctgaa
    21241 tatcagccgt tgattattca ttacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg
    21301 acggtgaccg actcagtgca ggatgtgcgc aacgccggga tgaccaacgc caaaccggct
    21361 attttactga tgatccgcaa actgccggaa gccaatatta tccagacggt tgacagcatc
    21421 cgggcaaaat taccggagtt gcaggaaacc attccggcgg cgattgatct gcaaattgcc
    21481 caggatcgct cccccaccat tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc
    21541 tcggtggcgc tggtgattct ggtggtgttt ttattcctgc gctcgggtcg cgccactatt
    21601 attcccgccg tttcggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
    21661 ggattcagtc tcaataacct ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg
    21721 gatgacgcca tcgtggtgct ggaaaacatt gcacgtcatc tggaagcggg aatgaaaccg
    21781 ttgcaagccg cactgcaagg tactcgcgaa gtcggtttta cggtgctgtc gatgagtctg
    21841 tcactggtgg cggtgttcct gccgctgctg ttgatgggcg gattgccggg ccgactgtta
    21901 cgcgagtttg ccgtgacgct ttctgtcgcc attggtattt cgttgctggt ttctctgaca
    21961 ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca agccgcgcga gcaaaagcga
    22021 ctgcgtggtt ttggtcgcat gttggtagcc ctgcaacaag gctacggcaa gtcactgaaa
    22081 tgggtgctca atcatacccg tctggtgggc gtggtgctgc ttggcaccat tgcgctgaat
    22141 atctggctgt atatctcgat cccgaaaacc ttcttcccgg agcaggacac tggcgtgttg
    22201 atgggcggga ttcaggcgga tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag
    22261 gatttcatga aaattatccg tgacgatccg gcagtggata atgtcaccgg ctttacaggc
    22321 ggttcgcgag tgaacagcgg gatgatgttt atcaccctca agccacgcga cgaacgcagc
    22381 gaaacggcgc agcaaattat cgaccgtctg cgcgtaaaac tggcgaaaga accgggggcg
    22441 aatctgttcc tgatggcggt acaggatatt cgcgttggtg ggcgtcagtc gaacgccagc
    22501 taccagtaca cgttgttatc cgacgacctg gcggcactgc gcgaatggga gccgaaaatc
    22561 cgcaaaaaac tggcgacgtt gccggaactg gcggacgtga actccgatca gcaggataac
    22621 ggcgcggaga tgaatctggt ttacgaccgt gacaccatgg cacggctggg aatcgacgta
    22681 caagccgcca acagtctgtt aaataacgcc ttcggtcagc ggcaaatctc gaccatttac
    22741 cagccgatga accagtataa agtggtgatg gaagtggatc cgcgctatac ccaggacatc
    22801 agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggca aagcgatccc gctgtcgtat
    22861 ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga atcatcaggg attatcggcg
    22921 gcctcgacca tttcgttcaa cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg
    22981 atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg tgcgcggcag ttttgccgga
    23041 acagcgcagg tgttccagga gacgatgaac tcacaggtga tcctgattat cgccgccatc
    23101 gccacggtgt atatcgtgct gggtatcctt tacgagagtt atgtgcaccc gctgacgatt
    23161 ctctccaccc tgccctcggc gggtgttgga gcgctgctgg cgctggagct gttcaatgcc
    23221 ccgttcagcc taatcgccct gatagggatc atgctattaa tcggcatcgt gaagaaaaac
    23281 gccattatga tggtcgattt tgcgcttgaa gcccaacggc acggtaacct gacgccgcag
    23341 gaagctattt tccaggcctg tctgctgcgt tttcgcccga ttatgatgac taccctggcg
    23401 gcgctgtttg gcgcgctgcc gctggtgttg tcgggcggcg acggctcgga gctgcggcaa
    23461 cccctgggga tcaccattgt cggcggactg gtaatgagcc agctccttac gctgtatacc
    23521 acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc gtttttcgcg taaacctaaa
    23581 caaacggtaa ccgagtaaat gacagatctt cccgacagca cccgttggca attgtggatt
    23641 gtggctttcg gcttctttat gcagtcgctg gacaccacca tcgtaaacac cgcccttccc
    23701 tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc acatggtcat tgtctcttat
    23761 gtgctgaccg tggcggtgat gctgcccgcc agcggctggc tggcggacaa agtcggcgtg
    23821 cgcaatattt tctttaccgc catcgtgctg tttactctcg gttcactgtt ttgcgcgctt
    23881 tccggcacgc tgaacgaact gttgctggca cgcgcgttac agggcgttgg cggcgcgatg
    23941 atggtgccgg tcggcagatt gacggtgatg aaaatcgtac cgcgcgagca atatatggcg
    24001 gcgatgacct ttgtcacgtt acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc
    24061 ggtctgctgg tggagtacgc atcgtggcac tggatctttt tgatcaacat tccggtgggg
    24121 attatcggtg cgatcgccac attgctgtta atgccaaact acaccatgca gacgcggcgc
    24181 tttgatctct ccggattttt attgctggcg gttggcatgg cggtattaac cctggcgctg
    24241 gacggcagta aaggtacagg tttatcgccg ctgacgattg caggcctggt cgcagttggc
    24301 gtggtggcac tggtgcttta tctgctgcac gccagaaata acaaccgtgc cctgttcagt
    24361 ctgaaactgt tccgtactcg taccttttcg ctgggcctgg cggggagctt tgccggacgt
    24421 attggcagtg gcatgttgcc ctttatgaca ccggttttcc tgcaaattgg cctcggtttc
    24481 tcgccgtttc atgccggact gatgatgatc ccgatggtgc ttggcagcat gggaatgaag
    24541 cgaattgtgg tacaggtggt gaatcgcttt ggttatcgtc gggtactggt agcgaccacg
    24601 ctgggtctgt cgctggtcac cctgttgttt atgactaccg ccctgctggg ctggtactac
    24661 gttttgccgt tcgtcctgtt tttacaaggg atggtcaact cgacgcgttt ctcctccatg
    24721 aacaccctga cgctgaaaga tctcccggac aatctggcga gcagcggcaa cagcctgctg
    24781 tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta tcgccgggct gttgctggga
    24841 ctttttggtt cacagcatgt cagcgtcgac agcggcacca cacaaaccgt ctttatgtac
    24901 acctggctta gcatggcgtt gatcatcgcc cttccggcgt tcatctttgc cagagtgccg
    24961 aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa ggagcgcgca atgaagttct
    25021 ggcgacccgg tattaccggc aaactgtttc tggcgatttt cgccacctgc attgtcttgc
    25081 tgatcagtat gcactgggcg gtgcgtatca gttttgagcg tggctttatt gattacatca
    25141 agcatggtaa tgaacagcga ttacaactgt taagtgatgc gcttggcgag cagtatgcgc
    25201 agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt
    25261 catttgaaca cgataattcg gaagataaac ccggcccggg tatgccaccg cacggctggc
    25321 gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct ggttggtccg cgagcgccga
    25381 ttccacctga cggtacacgg cgacccattc tggtcaacgg tgcggaagtt ggcgcggtga
    25441 tcgcctcccc cgttgagcgg ttaacgcgca atactgatat caatttcgat aaacaacagc
    25501 ggcaaaccag ctggttgatt gtcgccctgg caacgttact cgcggcactt gccacttttc
    25561 tgctggcgcg cggtttactg gcaccggtaa aacgacttgt cgatggcacg cacaaactgg
    25621 cggcgggcga tttcactacc cgcgtaacgc ccaccagtga agatgaactg ggcaaactgg
    25681 cgcaagactt caaccagctt gccagcacac tggagaaaaa ccagcaaatg cggcgcgatt
    25741 ttatggccga tatttctcac gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg
    25801 aagccattca ggatggcgtg cgtaaattca cgccggagac ggtggcgtct ttacaggcgg
    25861 aggtcggtac actgaccaaa ctggttgacg atctccatca gttgtcgatg tctgatgaag
    25921 gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc actgctggaa gtggcgggcg
    25981 gcgcatttcg cgaacgattc gccagtcgtg gcctgaaact gcaattttcc ctgccagaca
    26041 gtattaccgt atttggcgat cgcgaccgtt taatgcagtt attcaataac ttactggaaa
    26101 acagcctgcg ctacactgac agcggcggca gcctgcaaat ctctgccggg cagcgcgaca
    26161 aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt cagtgacgat cagctacaaa
    26221 aattgtttga acgtttttat cgcaccgaag gttcccgcaa ccgtgccagc ggcggttccg
    26281 ggctggggct ggcgatttgc ctgaacattg ttgaagcaca taatggtcgc attattgctg
    26341 cccattcgcc ttttggcggg gtaagcatta cagtagagtt accgctggaa cgggatttac
    26401 agagagaagt atgaccgagt taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga
    26461 agatgaaccg aagctggggc agttgctcat tgattatctg cgtgctgcga gctatgcgcc
    26521 gacgcttatc agccacggcg atcaggtact gccgtatgtg cgccagacac caccggatct
    26581 gatcctgtta gatctgatgc tccctggcac cgatggcctg acgctgtgcc gggaaattcg
    26641 tcgtttttct gacattccga tcgtgatggt gacggcaaaa atcgaagaga tcgatcgcct
    26701 gctggggctg gagattggcg cagatgatta tatctgtaag ccgtacagcc cacgggaagt
    26761 ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg cagcgcgagt tgcagcaaca
    26821 ggatgctgaa agcccgttga ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa
    26881 aatgcttgac ctgacgcctg cggaatttcg tctgctgaaa acgctctctc acgaaccagg
    26941 aaaagtgttc tcccgcgagc aattgctcaa tcatctttat gacgactacc gcgtagtaac
    27001 cgaccgcacc atcgacagcc acattaaaaa cctgcgccgc aagctggaat ctctcgacgc
    27061 cgaacagtca tttatccgcg ccgtttatgg cgtcggttac cgctgggaag ccgacgcctg
    27121 ccgcatcgtt tagttttagc gacattattt tgttagccgg agatggcgtt cagaccagat
    27181 ccggcaacat tatcccacgc atggtcagca aactgacatt atctctccgc cctggcaggc
    27241 atcagaaaat cgctcatact ttaatcggta aacagcacct ttagatgctg ttttcgatac
    27301 acaatttcaa tcaaggagtc attatggctg gttggtttga actcagtaag agcagtgata
    27361 atcagttccg gtttgtgcta aaagcgggca atggtgagac tatcctcacc agcgagcttt
    27421 atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag
    27481 aagaacgcta tgagaaaaaa acggcaagta acggcaaatt ctatttcaat ctgaaagccg
    27541 ctaatcatca aattatcggc tccagccaga tgtacgccac cgcgcaatct cgtgaaaccg
    27601 gaattgcctc cgttaaagcc aatggcacaa gccagacggt gaaagacaat acgtaattac
    27661 gatgccgggc gtgggttgtt ggcgtccggc gctttaccag atccccccac agcgctacaa
    27721 tgcccgccct taaagtggag gcactcccct aaccgcttca tcaggtgaag cggatctgac
    27781 ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg cgggaacgct
    27841 gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg
    27901 ttactccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc tcggcatcaa
    27961 tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc
    28021 caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc cggatgcgct
    28081 gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg aaatgccgat
    28141 ccacctctcg gtgcaggcta acgccgtaaa ctgggcgacg gtgaaattct ggcagcaaat
    28201 gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg aagagatccg
    28261 caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcactgt gcatggccta
    28321 ctccggtcgc tgcctgctct ctggctatat caacaagcgc gatccgaacc agggtacctg
    28381 caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg acgttggcaa
    28441 catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg
    28501 cgcgccaacc gacaaagtgt ttatgatcga agaagcccag cgtccgggcg agtatatgac
    28561 cgcgtttgaa gatgagcatg gcacttacat catgaactcg aaagatctgc gcgccatcgc
    28621 ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag gccgtactaa
    28681 atctttctac tattgcgcac gcaccgcaca ggtttaccgt aaagctatcg atgacgccgc
    28741 tgcgggcaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg
    28801 ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact acgaatacgg
    28861 ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc gcaaggggga
    28921 cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg agctgatgac
    28981 gccgcaaggc aacattaact ttacccttga gcacatggaa aacgccaaag gtgaagcgat
    29041 gccggtcgca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg atcttgagct
    29101 caattacgct ctgctgatgc gtaatttctc cggggaaacc acgcgtaacc cacacggtaa
    29161 gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat cacatacagc
    29221 tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa atgctagctg
    29281 taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat ttttttatgc
    29341 ataagcccta tctctggtaa ccgtcttcca ttgaccacat cgatagaatc ctccttcata
    29401 gcacgatgcc tttcacttat cggcatcgtg ctcccacaag ttccggctac gcacagccag
    29461 aacgcgcata tttgacgctt accaaaaaat attctcactc tccacatttg aatgtcagac
    29521 gagcgacacc atgtaatcct acaccttctg tcttcagctc aactatttgc atttttttgc
    29581 cctgagtaac acagaaatga gttgcatcat tttttactat attttctgca ccagatattc
    29641 tacccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa
    29701 tattactttt atatgcaaga ccaaatttac cgatacttgt ctcatcatta tgcacagcac
    29761 aaccagacat aataaaaata ctaattaatg atatagcagc tatctttttc atctcacctt
    29821 cccccattaa ataccaacga cactctctag tgtttaaata taa
//
LOCUS       DAZUGK010000035        29431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZUGK010000035 DAZUGK010000000
VERSION     DAZUGK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.035"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1306
                     /locus_tag="ACHY5W_001412"
     CDS             <1..1306
                     /locus_tag="ACHY5W_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS4446340.1"
                     /translation="SVKLSLLAVVVATIVSPSAFADDTVEAAATELTAIHPGMSHDEI
                     DQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPTSTAVQTDSTVTDKNQADRDA
                     AQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASADHA
                     NENANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAATADAQQSQSE
                     DQQTQITEQQKQIAATEKTVAAIGDPQSTAHYQKMINAKLTAQNEANERSTADQEQRI
                     NELASNVATQQQIDSVQYGEQIRNLAQDSAQAHEQIESLTQDSAQTHQQLTNTQKRVA
                     DNSQQINSLNNNFSSLKHEVEDNRKEANAGIASAVAIASQPQVKTGDFMMVSAGAGTF
                     NNESAVSVGASFNAGIHTVIKAGVSADTQSDFGAGVGVGYSF"
     gene            1407..1829
                     /locus_tag="ACHY5W_001413"
     CDS             1407..1829
                     /locus_tag="ACHY5W_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4446341.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1826..2032
                     /locus_tag="ACHY5W_001414"
     CDS             1826..2032
                     /locus_tag="ACHY5W_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446342.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            2121..3344
                     /locus_tag="ACHY5W_001415"
     CDS             2121..3344
                     /locus_tag="ACHY5W_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446343.1"
                     /translation="MLFPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVLYDPHPYGL
                     IRSLCWQDVPVIRFWSEAGCPTCAEFVYSGFADDEQGAARFLSALENWNRPWSGFTDA
                     FAALTPLFSCLADGYYLLEDRELYPTDGNGHFFWAATDHSASNPATVAVWDPEYCYFC
                     NTAPCFLLPGQPPSHFNPERVTFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATRFNDEQQTLVFPGGECLHKTELLCHVPKLTEWKTLPSGAWESFGP
                     DKHISPSETWSEELQQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADVI
                     LQALFFTHSPLFIDFARFVISYPAYVSYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKR"
     gene            complement(3345..3752)
                     /gene="cueR"
                     /locus_tag="ACHY5W_001416"
     CDS             complement(3345..3752)
                     /gene="cueR"
                     /locus_tag="ACHY5W_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFS4446344.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(3749..4924)
                     /locus_tag="ACHY5W_001417"
     CDS             complement(3749..4924)
                     /locus_tag="ACHY5W_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFS4446345.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTVVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAHEALRER"
     gene            complement(4921..7083)
                     /locus_tag="ACHY5W_001418"
     CDS             complement(4921..7083)
                     /locus_tag="ACHY5W_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFS4446346.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDDPSASLDEMAEAYLIEQLKQWIGHRTLVIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(7087..27864)
                     /locus_tag="ACHY5W_001419"
                     /pseudo
     CDS             complement(7087..27864)
                     /locus_tag="ACHY5W_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(27967..29322)
                     /locus_tag="ACHY5W_001420"
     CDS             complement(27967..29322)
                     /locus_tag="ACHY5W_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFS4446347.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5586 a         8570 c         8486 g         6789 t
ORIGIN      
        1 atctgtaaag ttgtctttac tggcagtcgt tgttgctacc attgtgagcc catctgcttt
       61 tgcagatgat acggttgaag cggcagcgac agaactgaca gcgattcacc caggtatgtc
      121 tcatgatgaa atcgatcaaa aaatcaatcg cttcatgatt cggacaggta atacagaagc
      181 cgcgcaagat tacctgtttt ctcatggata tatggaccca cagacaggcg ataatattta
      241 ccccaccagt acagccgtac agactgacag cactgtcacc gataaaaacc aggcagatcg
      301 cgacgcggcg caagataccg ccattcagaa tgcacacaat atcgcaagca ttgcggcggc
      361 aaatgcagat gacgccaaaa aagcgcttac cactgcgcaa tctgcaattg atgcgaacaa
      421 gggagctatt gctgatactc agaaagatgt cgcagatgtt aaagcaagtg cagatcatgc
      481 caacgagaac gctaataccg cgttgatgaa tggccagaaa ctttccggtg tcgtaagcga
      541 caacaaaaac cagattgaaa aaaacagtac ggcgattacc gaacagcagg aacaaatcac
      601 tgccacgcaa aaaaccgttg ccgccactgc tgatgcgcaa caatcacagt ctgaagacca
      661 acaaacgcag attacagagc aacagaagca gatcgcagcc acagaaaaaa cggtcgctgc
      721 aatcggcgat ccccagtcaa ctgcgcatta tcaaaagatg attaatgcca aactgacggc
      781 gcaaaatgaa gccaatgaac gttcaaccgc tgatcaggag cagagaatta atgaactggc
      841 ttccaatgtc gcgacgcaac agcaaatcga tagcgtgcaa tacggtgaac aaattcgaaa
      901 ccttgcgcaa gactcggccc aggcacatga gcaaatcgaa agtctgacac aagattctgc
      961 ccaaacgcac cagcagttaa caaacacgca aaaacgtgtt gcagataaca gccagcaaat
     1021 caattcgctc aataacaact tcagttcgtt gaaacacgaa gtggaagata accgtaaaga
     1081 ggctaatgcg ggtattgcgt cagccgtcgc tattgcttca cagcctcaag ttaagacagg
     1141 tgattttatg atggtgtctg ccggggcggg tacattcaac aatgaatctg cggtatctgt
     1201 cggtgcttcg tttaatgctg gaattcatac ggtaattaaa gcgggagtct ctgcggatac
     1261 acaatctgat ttcggcgcgg gtgtcggcgt ggggtattcg ttctaatttt aatagcaatt
     1321 tcttattata ctctaaataa ttcgagttgc agcaacttga agtatgacga gtatagcaat
     1381 attccgcagt ttatttaagg ttaacaatga tgaatagctc cattaaatcg ttttccctgc
     1441 tggcggttat attactggct ggctgtagtt cacccacttc ccgcatcgca gattgccagg
     1501 cgcagggcgt cagtcatgac acctgttacc tcgcagaaca gcagcgtcag gcggctattt
     1561 taagtgcatc cgaggcacag gcatttaaaa atgcagaagc cgcacaacac gcccaggcgg
     1621 caaagaaagc catttataaa ggatttggca tgacctttag aatgagcagt aaaaactttg
     1681 cttatctcaa tgattcatta tgtgcaattg atgaagacaa taaagatgcc actgtttatc
     1741 agtcaggtct gtataacgtc attgtttatc atcacacagg aaaagtcgcc ttaatgaaag
     1801 aaggccagtt cgtgggttat ttaaaatgaa ggagcaaaga aaaatacccc tgacgcatat
     1861 tatgattatc ggtgcgttta tttttgcctt cttgcaagta gtattattag cctccctggt
     1921 tcacgctgtg aatgttaaca atgaaatcca ggaaggctta tttcaatcgg ggcgcataat
     1981 ggtagaaagt ttgcagcata ttctttcggt gcaaacgggg attaatgggt aaggataccg
     2041 ttaccatttc cagagcaata gtcaccagcg taattttctg gcacagttca gggttctgaa
     2101 tattcggcta aggagtggtc atgctttttc cctttcgaaa acctgaagta cgtctgctga
     2161 aaaataccgc tgcgggcgtc attttttcgg tgaagtccgg taaaggattt ctgcgtcaat
     2221 gtgtgcttta cgatccgcac ccttatgggc ttatccgttc actctgctgg caggatgtcc
     2281 cggtaatacg tttctggtcc gaggccggct gccccacctg cgcggaattt gtctacagcg
     2341 gttttgcgga tgatgaacag ggagccgccc ggtttttgtc tgctctcgaa aactggaatc
     2401 gcccatggtc tggctttacg gacgcttttg ccgcgcttac accgctgttc tcatgccttg
     2461 ctgatggcta ttatctgctg gaagatcgtg aactttaccc gacggatggc aatgggcatt
     2521 tcttttgggc ggcaacggac catagcgcca gtaatccggc aaccgttgcc gtctgggatc
     2581 ctgaatattg ctatttctgt aacaccgccc cctgttttct tcttcctgga cagccgccat
     2641 cacattttaa tcctgagcgt gtgacgtttt atcgcgataa accggacgcc cgcgctttgg
     2701 cctggtatct ccctgattcc tatctctgcg ttctgctcga cgggcaccat aaggccactg
     2761 ctgccgcact ggaagggcga ccgctcaaaa cactggtact cagcacggca acccgtttta
     2821 atgatgagca acaaaccctg gtatttccgg gcggagaatg tctgcacaaa acagagctac
     2881 tgtgccacgt tcccaaactc actgagtgga aaacattacc gtccggcgca tgggagagtt
     2941 tcggccctga taagcacatc agcccatccg aaacctggtc agaagagttg cagcagagcg
     3001 tcagccggta cccttcactg gatcaagcct ggcaaattgt tgaggccggg aatttgagcg
     3061 aaactcgtat taagagcatg atccaacaag gtttggggga ggatgaaaag gctgatgtaa
     3121 ttttgcaggc gctgtttttc acacactcgc cgctttttat tgactttgcc cgattcgtta
     3181 tcagctatcc ggcatacgtc agttatcgcc ccctcacctt ccggttgatg gcgcaaaacc
     3241 gaacgccgca agcagatgct ttttttctgg attttgccat caatgatgac ggcgaacgtc
     3301 cggagttgac gaaaataatg gacgactatt ttcgcaagcg ttaatcaccc cgcccgatga
     3361 tgacagcagc cggagagatt ttcgataatc gggcagtcgg cgctgtcatc accggggcag
     3421 gcattcgcca gcgccagtag ctggttgcgc atagattgca gttcttcgat atgccgttcg
     3481 atctccgcga ctttctccag cgtgcgacgt ttgacgtcgg cactgtgacg ctgcgggtcg
     3541 ttaaacagat ttaccagctc gccgctctct tccaggttaa agcccacctg gcgcgcctgg
     3601 cgcagtaagg tcaattcgtt gagatgctgc tgcgtgtagg ttcgataacc attttcgctg
     3661 cgcatcggcg gcgtcaccag ccccttctct tcatagaagc gaatggcttt gctggtcagg
     3721 ccggtaattt ttgctacatc gctaatgttc atcgttcgcg caacgcctca tgtgctttgt
     3781 taaagggttt caacagataa tccagcacgc tttttttgcc ggtgcggata tcgaccgtcg
     3841 ccaccatacc ggggaatatc ggaaactgct gtttgctttt gttttccaga tggttgctga
     3901 aagtgcgaat gtacacccga tagtaataaa catcgcgccg cacttcatcc tgcacggtgt
     3961 ccggtgaaat caccgccact tcccccggca gaccaccgta gatggagtaa tcgtaggcgg
     4021 ttattttcac cagcgatttt tgccccggat ggataaacgc cacatcgcgc ggggatattt
     4081 ttgcctcaat cagcagttgt tcatccagcg gcacgatggt catcagttta ccaccggggg
     4141 caatgacgcc gccaactgtg gtgacgtcaa tatcctgaac aatgccacga actggcgcag
     4201 taaagttgag cctggtgagg gagtcttcac gcccacggat caccgaacgc tgggtttcca
     4261 cttccgcatt ggctttcgct aactcttcgc gcgcctgaac gtagtattgc gtgcgcacgt
     4321 cgctgagttt gttttccagt tcattggcct ggcgttgcag gcgcagcact tcgacgctgc
     4381 tcgccgcccc ctgtttcacc agcggctggg tcatcgccag ttcacggttg accaggctaa
     4441 tcccctgcgt cagcccggtg gtggctttgt tcaacccgtc gcggcgagtg ttgtacaacg
     4501 ccgtttccga ctcgataagt tcggggaatt tattcagttc gtcagggaag accagcggtt
     4561 tattactcac ttccgcccgc agccgcgccg aggttgccag cgccgcgtac agccgcgaca
     4621 tcgcttcctg gacgtcggat tcggttttgg tgcggttaag ctgcgccagc ggctggccct
     4681 gttcgacaat atcgccaact ttcacgtcga gatgatacag aattccgcct tccagcgact
     4741 gcaccacctg ctcgcgcccg gaaggttcta ccgtgccgct gccggtggtg acttcatcaa
     4801 gctggaagta ccacgcccag ccaagaaaac agagcagcat gacaacggtc acacgcactg
     4861 ccagcgccga acgcggcagg cggggttcag tcaggccacg gttatattgc agttgtccgt
     4921 tcatgcggca gaccccttat tcttttcctg caacaccacc cgtcgcgccg tgggttcgcc
     4981 ctgttcacgc aaaatggcct ctttcgcgcc atccatcaca atccgcccct gatccatcac
     5041 gataatgcgg tccaccagtt gcagcatcgc cgtgcggtga gtggcgatga ccagcgtgcg
     5101 atgcccaatc cactgtttca gttgttcgat taaatacgct tccgccattt cgtccagcga
     5161 ggcgctggga tcgtcgagca ggacgatatt cggctgagag atgagaagcc gcgccagcag
     5221 cagcgtttgc cgctggccgc cagagaggcc aaatccccct tcctgaatca tgtagttgag
     5281 gccgtttttc tgcttctgca caaagggcag cgctccgctg agggttaacg cgcgttcgat
     5341 atcttcatcg ctggcttccg gcatccccat ggtgaggttt tcccgcagcg aaccaaagaa
     5401 cagtcgcccg gtctggctga gtagccccat gtctcgccgc agatctgccg gatcaagctg
     5461 cccgatactg atgttatcca gcaacacctg cccctgctgg gcgatacgca ttccggcgag
     5521 catttgcagt agcgtcgatt tacccgcgcc gttgcgcccg agaatggcga ttttctcacc
     5581 agcctgaatg ttgagtttgc caatcgctac atcggcaatc ttctcttctt cgtcgtaata
     5641 aaagatagcg ttgctgaact gataattacc gtgcagcacc gctttatgca ccagtttgcc
     5701 gtgttccggc tgatccacgg ggcgcttcat cagttcatca aggccgttac gtgctacttt
     5761 cgcctgctgc cagcgcgaga gcacgccgga tatctgcgac agcggcgcga tggtgcgcga
     5821 agccaggatc gtcgtcccaa ccagtgcgcc ggtggtcatg tcgccgttca tcaccataaa
     5881 gcagcccacc aacagcacca cgacgtagac gatggattgc acttcctggg tccaggtgag
     5941 cagtagcccg gtgagaaaac gttgcttcat gctgatggac gaggccacgt cgttagtgtg
     6001 attccactga ttctgaaaac gctgttcagc gcgcagcagt ttaatatctt caatcgactg
     6061 cacggcttcc accagcgtag cgttgcgtac cgctgactcg cgcattcctt cgttcgccag
     6121 ccgcgccagc gggcgttgca ccagcaggcc ggggatcacc agcaacggca gcgctagcaa
     6181 aaccaccagc accagccagc cgccaatcat ccacagaata aaaacaaaca gcaggaagaa
     6241 cggcaggtcc gccaccgcgc cgatggtggt ggacgtaata agctcccgca ctgattccag
     6301 ttcgcggatc tgcgagataa acgatccggt ggatttcgat cgcacgttgt ttttcagccg
     6361 taatgcgtga ccaaaaacgc gatccgaaat acgcagatcc gcccgcttgc cgatcacatc
     6421 agaaagatgc gtgcgcacca tgcgcataca gaactcaaac aggatcgcca tcatcacccc
     6481 ggcgaacagc acccatagcg tcgggtacga ttgcgccgga accacccggt cgtaaacctg
     6541 catcgagaaa atcatcgccg ccagcgccag cacgttcgcc accagcgagg ccagcataat
     6601 atcgccgtag cgccgccagt ctttcagcga gagattccag aaccagttcg cctgatacgg
     6661 acgaatgtaa tcatctaccc gcgcgtcagg gatggcgctg agagggcgca acagcgccag
     6721 ttcaacgatt ttatcctcaa catccgcgac gttaagcgtg gtttccagcc cttcgtcgcc
     6781 gctgaactga acgctgatat tgtcatgatt gtcgcggcgg gtgattacgc caatctgctg
     6841 attatcaaat accgcgatca acggcaaccg ccacgggtta agcgaatcgg cggaaaactc
     6901 caccagccgt aaccccattc ccaactgccg cgccatgtcg gtgagtaact cctcccgctt
     6961 actgtcgcgc tcccagttga cggtcgcgcg cacattctcc tgagaaaaat caaggcggta
     7021 atagcgcgcc acggcgatca tcgcctccag ccaggcttcc acggactggg catttttttt
     7081 cattatctac accaccatca actgatgatt cgccagcaaa gtggcgaggt cggtgtgcac
     7141 gcccgtcagc gtgaccacgt tagttgtcgc aaaagtgccg cccgtgccgt cgcggtcgat
     7201 ctgcacgatg gtgtcgctgc cgctctgggt gactttgaca aaatcaccga tgtttccggc
     7261 ctgcgcatcc agcgttgcta cgccgttgac gtagctggct ttgccgtttc cggtgtagcc
     7321 gctgccctgt aacaactcgc ggatatcaat acgatcggtg tcggcggtgc cttcccatgt
     7381 gcctaccgta aagccattca ccacatccga accgttgccg ccagtggcgt cgctggcgtt
     7441 gagcagttta tagagcaagg tgtcatggcc gccgttgccg atgttgagcg tgtcgttgcc
     7501 gccgcgccct tccagttggt tatcgccgct gctgccggtc agaatgtcgt taaaattcga
     7561 gccggaaatg ccttcgatat tggtaaatgt cgaggtgtta aaaccggtgg cctgcgcggc
     7621 ggtgctgctt aagttagccg tcacgcccac cgtcgaaagc cgaaaatcaa ccacgtccat
     7681 cccgccgttc gcaagccacg ttccggtgcc ggagctgtac acccagccgc cggaaccgtc
     7741 gtaggtatcg gtgcctgcgg tggcgaagaa ggtgtcgtta tagccggtgc cgacgaactt
     7801 gccgttgttg ctcgcggtac ccgcttcagc gctgagatcg taagcgtgag tggcatcggt
     7861 agcctgtaaa ccgccccagc tggtgttgac cgtctggtcg atattgctgg cgttggtgcc
     7921 ggtggatttg atcagcaccg cgttgtaggt ttctccggcg ttcagcccgt agaagttgac
     7981 cagcgccgag gtgtcgttaa cccccatacc cgactgcggg ttgatgattt gcgtggcgac
     8041 cagcactccc gccgagttgt acaacttcac ggtattgccg taatagacgt taatgccgtt
     8101 gggatcggtg atgcgcaggt gtagcgaagt gccgtagctc accgtgttgg tgttgcggat
     8161 caggaacacg ctgcccgact gtttggtcac cagcacatcc accgcgccgt cccagtcgta
     8221 atccattgcc gccacgccgc tggtggtacc gctctggctg ccgccgaggg ccgattgtgt
     8281 ccagttgctg gccccgccaa cattggtgta aaggtaggat tgtccggtct gggcgatacg
     8341 ggcgatatcc atcagaccgt catggttcca gtccaccgcc aggctaaact gactggcgta
     8401 ggtcgtcgcc gttacgccca ccgccaccgg acttcccagc acgccgttgg tgttgaataa
     8461 cagcgagccg tagttagctc ttccctggct ggcgggcagg aagagatcca tatcgccgtc
     8521 accatcgaaa tcggcccagg tcatagaaac gctgctgctc atagcgccac tacccgtccc
     8581 gctcaggaag gtattggtgg tgttctgccc ccagacaaac gtcccattcc cttggttgat
     8641 gagcgaagat agcgtgtaat agttgttcag gttgtaggtg tgctgaacca ggtcgatcct
     8701 gccgtcattg ttcagatcga cgccagaacc ttcgttgagc gaaagatacc ccgtcaccgc
     8761 cccgcccacc gtggcgctac cgccgctgtt cgacgtggtg gagttaccta ccagcgtacc
     8821 tgcgttgttc cacaggaagg tgttggagtc cggcccgccc gcgtcgccaa tccagaagtc
     8881 gagatagccg tcgccctctt tatcaaatgc cacgatcgag ccataccacg tcagcgtgcc
     8941 gacggtgacc tgaatagcgc tggaaaaggt gccatcgccg ttattggtaa agccgttaat
     9001 atagcccgta ccgtaatcat cacgggtgat aaacagatcg cccgtgccgt tacggtcgta
     9061 atcggcaatg gagctggtgg cgtagttgtt gccgctctga tacagcgtca gcgcggtctt
     9121 cgaccaggtg agtgggtcag tcgattgcat tacctgctgg tttgccagca ccgtccacat
     9181 cccgtgcgag tcgaggccgt aggtcaaccc ccaggcggtg gttttgctgg cggtagtcca
     9241 ggtcggtgtg taatcaatcg ccgcgttaac cgtcaccgtg ccgttgctaa tattggcgtt
     9301 attgccgtta cccgccgaac tttttacctg cgcagtaacg gtataggcgg tcgcggaaac
     9361 tgtcagcgca tcggtatccg gcaactgtac ataccaggtg ttgtgcgccg gatcgaccac
     9421 taccgcgccg cccggttcag aggtgtaggt tttgccgttg atcaccactt caacatactg
     9481 cccactggca agcgcggcgg tgatcacccc gctgataatc ggcgtagtat cgcgcgtggt
     9541 ctggctggtg atttgcgcca gcgtggtcgg aatagaggta tcgaccgtca ggacaaaatc
     9601 actggaggag gtgatattcc ccgccaaatc caccactcgc gcgctgtagg tataggcacc
     9661 gctcaccagc ccgctgtcgc tgtaggtcca gttcagagcg ccgaccatcg tcaccgcccc
     9721 taacagcagc ccgttacggt agaggtaaac cacttcaccg ctggcaagcg gcgcggaaag
     9781 tacaccgttc agcagcggcg tagtgtcgtc ggtggcctgc gaactgctta atgtcccctg
     9841 ccgctgcccg acatcatcgg tatacgacac aatacctggc gtggcggtag ggttaatcgt
     9901 atcgaccacc accgttttcg ataccaccgg gccggtgttc cccgccagat ccagcacccg
     9961 aacctgataa acgtagctgc cgtcggtcag cgtgcggctg tcggtgtagg tccactgtgt
    10021 accgttggtg gtaagcgtga tccaggtcgc gccgctatca aggctaatct gcgccacttc
    10081 gccgctggca agccccgctc ccagcgaacc ggtcagcgtg agcgtggtat cgctggtgat
    10141 aaagtcagtt gccgaactcc ccgtgtcggt gctaatgctg gcgatggtga gcacctgcgc
    10201 cgggttaacg gtgtcaatct gcgccgactg gctgccggtt gcgccaacgt tgcccgccag
    10261 atcgaccacc cgcacctgcc aggtggtggt gccgttggtg agggttcgtc cgtcaacgta
    10321 actccagttc aggccgtctt ctgctagcgt cacgttcacc caggttgcgc cgttgtcggt
    10381 actgatttgt gcgaactcat tagcgccgag cgccgcgccc aacacgccgc gcacggtaag
    10441 cgtggtgtcg ctggtaataa aatcgctgct gctggctccg gtgtcatcgc tgataccggt
    10501 aatggtgata cttttcgccg cttccgggct ggtggtgtcg accaccacat tttgcgtcgc
    10561 ggtctgcccg acattccccg ccagatccac cacccgcacc gtgtagttcc aggtgccgtc
    10621 agtaagcgtg cgaccatcgg catagctcca gctggtgccg ataacggtta acgtagtcca
    10681 ggtgacgccg ccgtcgaggc tgatttgcgc ccgttcatct gccgcaagcg ccgcgccgag
    10741 cgtgccgcgc acggtcagcg tggtgtcgcg tgtaacaaaa tcgctgctgc ttaacccggt
    10801 gtcatcgctg atgctcgcaa tggcgatggt tttgctcgcc agcggcgcgg tggtatccac
    10861 agtgactatc tggctggcag tcgcgccgat atttccggcg gtgtcgatca cccgcacctg
    10921 gtagttgtag ttgccatcgg tcagcgtacg cccgtcaaga tagctccagg tcaaaccgct
    10981 taccgaaaga ttctgccagg tggtgccgcc atcaatgctg atttgcgcga actcaccggc
    11041 agaaagcgtc gcccccagcg tgccgctcac cgcaagcgtc gtgtcgctgg tgacaaaatc
    11101 gttagtgatc aagcccgtat cggtggtgat cgcgctgatg gcgatggttt tcaccgcggg
    11161 atctggcgcg gtagtgtcga tcaccacatt ctggctgtcg gtcgcgccaa cgttgcccgc
    11221 tacgtcaatc acccgcacct gatagagata gttgccatcg gtcagcgtgc ggctgtcgtt
    11281 atagcgccag gtcgtgccgg taacggtaag cgtggtccag gttacaccgc cgtcgatact
    11341 gatttgcgcc gtctcgttac tggcaagcgt ggcggtgagc gagccgttga ccaccaccgg
    11401 gctgacgctg gtgataaagt cactggcgct aaggccagtg tcggcttgca gggaatcgat
    11461 attaataccc atcgccgggg cgctgagatc gacggtaatg gtgtgattgc tgctgccggt
    11521 attgccaatg gcattgctca ccgaggcgtt aatcacgtag gcgttgccgt ccgccagcgc
    11581 ggtgacatcg gcgctgccga gggtgtagct ccagctaccg cccgtctgca ccgtggtggt
    11641 gtaggttttg ccgtttaagg tcagggtcag cgtctgcccg acgggcgcgg tggtggtgcc
    11701 gctgatcacc agcgatgagc cgtgttccgc tgcattcacc acatcgtcgc ccgcaaaggt
    11761 gttgatggtg acggtgggca cgtcgccgtt cagcgtcacg ccacggctgg cgctgccggg
    11821 gtttccggcc tgatcgctaa ccgtcgcgct aatggtgtag ctgccgtcgc ttaatccggc
    11881 aaactgctgc gccggaataa acaccgacca gcttccgccg ctgccaaccg ttgcgctcca
    11941 ggtttgcccg ttaaaggtga cggtcaccgt ctggcccact tcggcggtgg tggtgccgct
    12001 gatggtctgc ccggcaagct gctcggcgtt gttgatgata tcgtcacccg ccacggtggc
    12061 gatggtgacc gttggcgcca cggtgtcgac ggtcagggcg tgcgtggcct gtccggggtt
    12121 accggcgcga tcgttcacgc tggcggtaat ggtctgcgcg ccctgcccga gcgttgccag
    12181 atccgccgca ggcacgttca gcgcccaggt gccattggcg gcaaccgtgg cagtgtaagt
    12241 tttgccgcca aaggtgacgg tgaccgtctg ccccacttcc gcgctggtcg agccgttaag
    12301 ggttaacggc tgctgctgtt cggcggcgct gagtcgatcg tccgtcgaaa cggtgttaat
    12361 gctgataacc ggcgcagtga gatccaccgc cacgctgcgc gaggccgtgg cgctgtttcc
    12421 ggcactgtcc tgagcggagg cgctaaccgt gtagctggtg ccatcactga gcgccgcaac
    12481 atccgccgca ggcacgttca cgctccagct cccgttgctc tgaatggtgg cgctgtagcc
    12541 tttgccgtta agcaacacgg tcaccactgt acctgtcgcg aaattagtgc cagtgccgct
    12601 cagggtcagc gcactgcccg cttcggcggc gttaataata ttatcgccgc tgatggtgct
    12661 gaccgaaagc gacactgccg ccgtgttcac ctgcacgttg tgcgtctgcg tgctgctgtt
    12721 gcctgcgcta tcggtaatgg tggcgctgat ggtcaccgtg ccatccgcaa ggccggaaat
    12781 cacgctggca ggcacgccga cgctccagtt gccgctggca tcggtgctgg tgacatactg
    12841 ctgcccggcg atggtaacca ccaagcggtc gcccgcgacc gcgcccgtgg tggtgccgct
    12901 gataatctgc gcctgagtgt gttcgacgtt gttaatcaca tcatcgcccg ccacggtgtt
    12961 aaaactgatc accggcgcag tggtatcgac ggtcacgcct ttgctggcgc tgccagggtt
    13021 acccgccaga tcgctgaccg tggcggtcag ggtgtagccg ttattagcaa gtgcggttaa
    13081 gtcggtggcg ggcagcgtta aactccagct gccgtctgcc tgcaccgtgg tctggtagtt
    13141 agtgccgtta agcgtgacgg tgagcatctg cccggtttgc gccgtgctgg tgccggacac
    13201 cgttacgccc gccgccgctt cgctggcgtt gatgatgtta tcgctggcaa tggtgttgat
    13261 ggtgaccgtt ggcgcggcgc tatcgacgct gtattcatgc gtggctgtgg cgctgttgcc
    13321 gttgacgtta gtcacccgca cctgggcgct ggcatcgccg tcacgcagcg ccgccaaatc
    13381 tgcagccgga acagtggtgc tccagctacc gtttgccgcc accgtggtgg tgtaggtttt
    13441 accgccaaag gtgacggtga cggcttgccc cgcttcgacg ccggacgtgc tgccggagag
    13501 cgttaatgcc gcgccttttt cggctgcgtt aatcacatca tcgctggtaa tgctgttcat
    13561 gctgatggcg acggtagtga gatcaacagt cagcggatgg gtaatgctgg tttgcgttcc
    13621 ggcactgttc gcgccgctaa cggtaatgtt cagcgttccc gcaggccagt tactgacatc
    13681 cgccgccgga acgccgacgc tccagctgcc gttcgccagc accgtggcgg cgtaagtttg
    13741 attatttatc accaccgtca gcgctgtacc tgcggcaaag ccggagctgc tgccagtaat
    13801 cacctgcgcc tgagcgtgtt cgataatatt gactacatca tcacccgcga cggtatcgac
    13861 tcgcagacct ggcagactgg catcgatggt gatatcgcgc gtgccgctac cggtgttgcc
    13921 aacggcgttg gtgaccgagg cgttaacggt taactcaccg ttacccagcg cctgccagtc
    13981 ggcggctggt acgctcacgt tccagcttaa atcaacctgg acgatggcgg tgtaagtttt
    14041 accgcccagt tcaacggtga cggtactgcc cgccgccgcc ccggttacct ggccgctgat
    14101 ggtttgtgcg ctgcccgctt cggcggcatt gataatatcg tccgtcgcca ccggattaag
    14161 ggtgacgccc ggcagcgcgg tattgacctg cacgttatgg ctggcggtgc tgctgttgcc
    14221 ctgggcattg gtgacgctcg ccgtgacgct gtaggtcgct tcgcccagcg cactgaccgc
    14281 cgaggcaggc acggtaacac tccagttgcc ggaggcgtcg gtggtggcgc tgtagttttg
    14341 tccgttcagc gtgacggtga tcgtcgtgcc cgctggctga tcgctggtgc cagaaagcgc
    14401 cagatcagat cctttttccg tcgcgttgat cacatcatcg cccgcgatgg tgttgatgct
    14461 aatcactggc gcgctgaggc tgacggtcac ggtgcggctg gcgtcgtcgc tgttgcccgc
    14521 ccgatcgctg acgctggcgg tgatggcctg tgcgccgcta ccgagtgccg caacatccgc
    14581 tgccggaacg ccaacgctcc agttaccgga ggcatccagc gtggtggtgt aggttttgcc
    14641 gttgagcaca acgctcacca catcgcccgc ttccccgccc gtgctggtgc cggaaatcac
    14701 cagcgcctgg gcgtgttcgg cgtcattgat gatgtcatcg cccgccacgg tatttatggt
    14761 aagaaccggc gcggtggtgt ctaccgccag attatggctg gcgctgccgg ggttgcccgc
    14821 tttgtcgttg accgtggcgc tgacggtgta attgcttgcg ctcagcgcgc ccagggctga
    14881 actgggaacg ctgacgctcc agctaccgcc cgcctgtaca ttaccggtgt aggttgcacc
    14941 gttaagggtg acggtgaccg tctgcccggt ttcggcggtg ctggtaccgc tgatggtgag
    15001 cgggcttccg gcctctgcgg cgttaaggat gtcatcgctg gcgatggtgt ttatggtgag
    15061 cgtcggtgcg ctggcgtcaa cgctgtacgc atgggtcgcc gaggcgctgt tgccgctcgc
    15121 gctgctgacg ctggcctgca cgttcgccgc gccgtctggc agagatgcca gatccgccgc
    15181 cggaacattc acgctccagc ttccgttggc ggcgacgctt gcggtgtagg ttttaccgcc
    15241 aaaggtgacg gtaacggttt gaccgctttc aatcccgctg gtgctaccgg aaagggtcag
    15301 gtcggtgcct ttttcggcgg cgttaatcac gtcgtcgctg gcaacggtgt tgatgctgat
    15361 tgccaccgcc gttaaatcga cggtgaacgg atggctgacg ctgaccggat ttccggcgct
    15421 gctctggcct gccacatcaa cggttaacgc ccccgcaggc caggcgctga cgtttgccgc
    15481 cggaatgccg acactccagc ttccgtccgc ctgtaccgtg gcggaataag gcgtaccgtt
    15541 aatcgtgatc gtcaacggca cgcctgcatt caggccacta ctgccaccag tgatcaccag
    15601 cgcctgtccg tgttcaatgc tgttgacgat atcatcgccc gccacggtat ctacgcgcag
    15661 ccccggcagg ttggcatcaa tggtgatatc ccgcgtgccg ctcccggtgt tgccattagc
    15721 gttggtgacc gaggcggtaa tggtcaaatc accattgccc agcgcctgta aatcggctgc
    15781 cggaacggtg acgctccagc ttaaatcggg ctgaacctgc gctgtgtaag tgttgccgcc
    15841 cagcgtcacg gtgaccgtat cgccagcggc ggcgcgagtc accaccccgc tgatggtttg
    15901 atcggccccc gcttcggcgg cgttgataat atcgtcgcct gccacggtgt tgatggtgac
    15961 gtccggcagg ccgctgtcga ccagtaaatt cgcctggctg ctggcggtgt tgcctgctgc
    16021 actggtggcg ctggcgctga ccgtatagtt ggcctgacct aatgccgcca gatcgctcac
    16081 cggcacggct aaggtccagt tgcccgacgc gtcggtggtg gcgctgtagt tgtgaccgtt
    16141 gagcgtgacg gtgacctgcg cgcccgtcgc cagccccgta ctgccgccgc tgatggtcaa
    16201 cggctggcct ttttcatcgg cgttcaggac gttatcgccg ctgatggcgt taaaggtaat
    16261 ggtcggcagc ccggtattga ccgtcacctg atgggtggtg ctgccactgt ttcctccggc
    16321 atcagtgacg ctggcgttaa tggtcaccgt gccgttcgcc agtgccgaga cgacgcttgc
    16381 cggaacgccg acgctccagt tgccgctggc atccagcacc gtggtgaaag tatttgtgcc
    16441 gatagtcacc gtcacggtgc taccggttgc cacacccgtg gcggatccgc tgatgatctg
    16501 tgcctgtgcg tgttccgtcg cgttgatcac atcatcgcct gccaccgtgt tgatggtgac
    16561 gaccggaaca gaagtatcca ccgtcaggtt gtgattaacc gaggccgggt ttccggcttt
    16621 gtcgctcacc gccgcgttca cggtgtagtt gctggcggtc agggcgctta cgtcggcttg
    16681 cggcacactg acgctccagc taccgtccgt ttgtacggtg ccagtatagt tttcgccatt
    16741 gagagtgacg gtcaccgtct gccctgcttc cgccgtgctg ctgccagtga tggtcagcgc
    16801 cgctccggct tcggcggcgt taagaatgtc gtcgcccgca atggtgttaa tggtcaccgt
    16861 tggcgcgctg gcatcgacgc tgtaagcgtg ggtcgtggtg gcgctgttgc cgttaacatt
    16921 gctgacgctg gcctgtgcgc tggcatcgcc atcacgcagg gccgccatat ctgccgccgg
    16981 aaccgaggtg ctccaggaac cattcgcagc caccgtggcg ctgtaagttt tgccgccaaa
    17041 ggtgacggtg accgtttgtc cggcttcgac gccagaggtg ctgccggaga gtgttaacgc
    17101 cgcgcctttt tcagcggcgt taatcacatc atcgacggta atggcgttga tgctgaccgc
    17161 caccgccgtg agatcgacag tcaccggatg cgtaacgctc accgggtttc cggcggtggt
    17221 gttgccgctg gcggcaatcg tgaccgtccc cgcaggccag gcgctgacat ctgccgccgg
    17281 aacgccgacg ctccatgagc catccgccag caccgccgca acatacgttt gcccatttat
    17341 ggtcagcgtg acgttgctgc ccgctgccag atcggagctg ctgccggtaa tcaccagcgc
    17401 ctgattgtgc tcgatgatat tgaccacatc atcgcccgcc acggtgtcaa tccgcagacc
    17461 gggcagattc gcgtcgatgg tgatttcacg cgtgccgtta ccggtattgc ctacgctgtt
    17521 cgttaccgaa gccgaaatgg tcagctcgcc gttgcccagc gcctgtaacg ccggggccgg
    17581 aacatcgatg ctccagctta aattcgcctg caccgtggca gtgtaagtcg ccccgccgag
    17641 cgtgacggtc accgtgtcgc ctgccgccgc acccgtcacc tacccgctga tggtctggtc
    17701 cacgcccgct tcggcggcgt taattatatc gtccgttgcc accacgttaa tggtgacgcc
    17761 aggcagcgcg gtattaacct gcacgttatg gctggcggaa ccgctgttgc catcgacgtc
    17821 agtggcggag gcggtcaccg tataagtggc ttcaccgagc gcgcttacgg cggacgccgg
    17881 aacggtcacg ctccagttac cggatgcatc ggcagtggtg gtgtaatttt gcccgttgag
    17941 cgtaacggta atctgcgtgc ccgcgggctg gttgctgctg ccggaaatcg ccagatctgc
    18001 gcctttctcc gtggcgttga tgatgtcatc gacggcaatg gtgttgatgg cgagtaccgg
    18061 agcaccgagc gccacggtga cagtgtgcga ggccgtgccg ctgttgcctg cggagtcagt
    18121 aaccgtggcg gtaatggtca catcgccctg cgccagcgcg gaaatgacgc tggcaggcac
    18181 gccgacgctc cagttgccgt tggcgtccag cacggtggtg taggtggtcg agccaatcgt
    18241 cacggaaacc gtgttgccgg tcgtcgcgcc cgttgccgag ccgctgataa tctgcgcctg
    18301 agcatgttca gtggcgttaa tgacgtcatc gcccgccacc atgttgatgg tgacgaccgg
    18361 agcggcaagg tccaccgtca gattatgcgt tgccgaagcc ggatttccgg ctttgtcgct
    18421 gaccgccgca ctgaccgtat aggggctggc ggtgaggtta gctaaatcgc cggtcgggac
    18481 gctgacgctc cagcttccgt ctgcctgcac gttgccgctg taattgacgc cgtttagcgt
    18541 cacggttacc gtctgccccg cttctgccgt gctggtgccg ctgatggtca gcgccgatcc
    18601 cgcttcggcg gcgttaagaa tatcgtcgga ggcaatcgta ttgatggtga cagagggcgc
    18661 ggtggcatcg acgctgtaaa catgtgtggc ctgggcgttg ttacctgcga cgttgctgac
    18721 gctggcctgg acgtttgctg cgccatctgg caaggttgcg agatcggcgg cagggatcgt
    18781 cagcccccag ctattatccg cggcaaccgt ggtggtgtag cttttgccgc cgaagataac
    18841 ggtgatggtc tgccccgctt ccacgccgga ggtggtgccg gaaagctgca aatcagagcc
    18901 tttttcagcg gcgttaatga catcgtcagt ggaaagggtg ttgatggtaa tcgccacgac
    18961 cgcgagatcg accgtgaccg gatgagtaat gctggttgtg gttccagcag tattagtgcc
    19021 tgaaacagca atattgaccg ttcccgcagg ccagttaccc acgtctgccg ccggaacgcc
    19081 cacgctccag ctgccatcgg tgagtaccgt tgcgccgtag gtgacgccgt tgataaccac
    19141 cgtcaacgcc gcgcccgccg ccagcccggt gctactgccg gtaaccacca acgcctgagt
    19201 gtgctcaatg ctgttaatca catcatcgcc tgccacggta tcgacgcgca gacctggcag
    19261 gttggcatct atcacgatat cgtgcgtcgc ggtgccggta ttatttgcgg aattggtcac
    19321 cgaggcgctg atagtcagtt caccgttacc cagcgcctgc aaaacgttcg ccggtacgct
    19381 gacgctccag cttaaatctg actgcacggt ggcgttatat tgattgccgc caatggtgac
    19441 aatcaccgta ttcccggcaa ccgccgttcc cgtgacctga ccgctgatgg tctgcgccac
    19501 aacgatttca gcggcgttga taatgtcatc gcccgcaacg gtattaatga tcaccccagg
    19561 caggagcgat tcgacattca ccgtatgggt ggtgctgccg ctgttgcccg cgctgtcggt
    19621 ggcgctggcg ctaatctgat acaccgcctc ccccagtgcg ccgaccgccg aagcaggcac
    19681 ggtaacgctc cagttgccgg aactatcggt ggtggcgctg tacgccaggc cgttcaacat
    19741 cacggtgacc gtggtgccag tcgccagacc gctggagccg ccgctgatgg tcagatcctg
    19801 ggttttctcc gcattattaa taatgtcatc gccggagacg atactgatgg tcagcgtcgg
    19861 gccgctgaga tcaacggtca cattatgggt agtgctgtcg ctgttgcccg cgcgatcgct
    19921 caggctggcg gtgatcgtct ggctaccggt cgccagcgcc gtcacatccg ccgccggaat
    19981 gcccacgctc cagttaccgg aggcgtccag cgtggtggtg tagtttttac cgttcagcgt
    20041 gacggtcact acatcgccgg gttccgcgcc cgtactgatg ccgctgacga ccagtgcctg
    20101 cccgtgttcg atggcgttga tgatgtcatc gcccgaaacg gtgtttatgg tgagatccgg
    20161 cgcggtaacg tccaccgcca gcgcgtggtc ggcgcttgcc gggttacccg ctttgtcgct
    20221 caccgtggcg gtcacggtgt aactgctggc ggttaatccg cttaaatccg ctgccggaac
    20281 attcacgctc caggtgccat ccgcctgaac ggtggtctgg taagtgttgt tattgagcgt
    20341 gacggccagc gtctgtccgg cctgcgccgt ggtggtgccg ctgatggcga tccccgcgcc
    20401 cgcttcgctc acgttaagaa tatcgtcgct ggcgatggtg ttgatggtca caagcggcgc
    20461 ggtggcatcg acgctgtacg cacgatccgc ctgggcgctg ttgccgttaa tattgctgac
    20521 gctcgcctgc acgttgcccg cgccgtccgc cagggcagcg agatcggcag gcggcacatt
    20581 caccgtccag ctaccgttag cttccacagt agtggtgtaa tttttgccgc caaaggtgac
    20641 ggtcaccgtc tgcccggctt cgacaccgct ggtgctgccg cttacgacca gcgtttcgcc
    20701 tttttcgacg gcgttgatca catcatcacc agagagagtg ttaatggtga tggcgacccc
    20761 agccagatcg acggtaaccg gatgggtaat ggtggaggtt gttccggcac tgttggtgcc
    20821 gcttaccgtg atattcaccg tacccgctgg ccagttgctg acgtctaccg ccggaacacc
    20881 gaggctccac gtgccgtcgg ctaataccgt cgcgccataa gtaacgttgt taatctcgac
    20941 cgttaacgcc gttcccgccg ttaatccgct gctgctgccg gtgatcacca gcgcctgatt
    21001 gtgctcaatg ctgttaataa catcatcgcc cgccacggta tcgacccgca ggccaggcag
    21061 attggcgtcg atcgtgatat cgcgcgagcc gctgccggtg ttgccgacgc cattggtgac
    21121 cgaggcatta accgtcaggt cgccgttgcc cagcgcctga atatgcaccg ctggcacgct
    21181 gacactccag cttaaattag cctgcaccgt ggcggtgtag gtgttgccgc ccagcgtgat
    21241 ggtaacggta tctcccgccg cggcacccgt cacctgcccg ctgatggttt gcgcattgcc
    21301 cgcttcggca acgttaataa tatcgtcagt cgccaccgcg ttaatggtaa cggcaggcaa
    21361 ggcgctgttg accagcacat tatggctggc ggaattactg ttgcctgccg tgtcggtgac
    21421 gtttgccgtt accgtgtagt tagcttcacc caatgcgcta actgccgagg ctggcaccgt
    21481 ggcgctccag ttgccggaag aatcggttgt agcggtgtaa ttttgcccgt tcagcgtcac
    21541 cgtaatggtg gtgcccaccg gttgattact ggtgccggta atttgcagat cagcgccttt
    21601 ttccgtggcg ttaatcacat catcgctggc gatagtgttg atgccaatgg ttggcgcggc
    21661 gagacttacg gtgaccgtgc ggctggcatc atcgctgttg cctgccgcgt cggtaattgc
    21721 cacagtgata gtttgcgggc cgctgccaag cgcagtaacg tctgccgccg gaacgccgac
    21781 gctccagttg ccggcggcat ccagcgtggt ggtgtagttt ttactgttta acgtgacggt
    21841 gatgacatcg cccgcttcgc cgccagtact ggaaccggag atcaccagcg cctgtccgtg
    21901 ttcggcggca ttaataatgt catcgccgga gacagtgttg atggtcagca ccggcacggt
    21961 gagatccacc gccagcccgt ggttagcgga cgccgggtta cccgctttat cgcttaccga
    22021 ggcgctgacc gtgtactggc tggcggtcag attgctgaga tccgttgtcg gtacgctgac
    22081 gctccagctc ccgtccgcct ggacggtgcc ggtgtaagtc acgccattaa gggtgacggt
    22141 taccgtctgc cccgcttcgg cggtgctgct gccgctgatg gttagcggat tgcccgcctc
    22201 ggcggcgtta agaatatcgt cggtggcgat ggtgttaata gcaagcgtcg gggccgtggc
    22261 atcgacgctg taggcgtggg tcgccgaagc cgtgttgccg ttaatagtgc tgacgctggc
    22321 ctgtaccgtg gcgtcgccgt cgcgtaatgc ggcgagatcg gcggcaggta cgctggttgt
    22381 ccaactgcca tcgcctgcta cggtggcgtt gtaggttttg ccgccaaagg taacggtgac
    22441 cgtttgcccc acttccacgc cggaggtgtt gccggaaagg gttaaatcgg cccctttttc
    22501 agcggcgtta atcacatcgt cgccggaaac ggtgttaatg gaaatcgcca cagctgccag
    22561 atcgacggtg actggatggg tgatagtcgt agtcgtttct gcagtgttag tgccgcttac
    22621 aatgatattc actgtacccg caggccagtt gcccacgtct gctgccggaa cgccaaggct
    22681 ccatgtgcca tcggctaata ctgtcgcgcc ataggtgacg ttattgatca ccaccgtcag
    22741 cgccgcgccc gccgccagcc cgctgctgct accggttatc accagcgcct gagcgtgctc
    22801 gatgctattg accacatcat cgcccgccac ggtatcgacg cgcaggccag gcaaattggc
    22861 gtcgatagta atatctcgcg aaccgctgcc ggtattgcca acgccgttgg tgaccgaggc
    22921 gttaaccgtc aggtcgccat taccgatggc ctgaatatcc gcagccggaa cgtccacgct
    22981 ccagcttaaa ttgccctgca cggtagcggt gtaggttttc ccgccaagcg ttacggtaac
    23041 cgtatcgccc gccgccgcac ctgtcacctg cccgctgata gtctgcgcca cgcccgattc
    23101 ggcggcgtta ataatatcgt cggtcgccac cgcgttaatg atgacgccag gcagcgcggt
    23161 attaacctgc acgttatggc tgacggaatt gctgttgcct gcgctgtcgg tgacgttcgc
    23221 cgtcaccgtg tagctggctt cgcccagcgc accgacagcg gaagctggca ctgtagtgct
    23281 ccagttgccg gaagcatctg tggtggcagt gtaattttgc ccgttcagcg tcaccgttat
    23341 tgtggtgcct gctggctgat tgctggtgcc ggaaatttgc agatctgcgc ctttttcggt
    23401 ggcgttaatc acgtcatcgg tggcgatagt gttaatgcca atggtcggcg cggtaaggtt
    23461 aacggtgacc gtgtgcgttt cgctgtcgct attacctgcc gcatcggtaa tgctggcagt
    23521 gatggtttgc gggccgctgc ccagcgcagt gacgtctgcc gccggaacgc cgacgctcca
    23581 gttgccggag gcgtccaggg ttgttgtata ggttttactg tttagcgtga cgctaacgac
    23641 atcccccgct tccccgccag tgctggagcc ggaaatcacc agcgcctgcc catgttcggt
    23701 ggcgttaata atgtcatcgc ccgcaatggt gttaatggtg agtaccggaa cggtgagatc
    23761 caccgccaga ttatgcgtgg tgctcgccgg gttgcccgct ttgtcattga ccgtggcggt
    23821 cagggtgtgg ctgccatcgg tcaaggctgc gagatctgcc gccggaatat tgatgctcca
    23881 gctgccgtcg gcctgtaccg ttgcctgata cgtctgcacg gtcgggctat tgagcgttac
    23941 cgtaacaatc tgccccgctt cagcggtggt gctgccgctc accgtcacac ccgcatcggc
    24001 ttcgctggcg ttgacgatat tgtcgctggc aacggtattg ataatgatgg tcggggcact
    24061 gctgtcgacg ctgtagttat gcaccgccga ggcgctattg ccgttgatat tactgacgct
    24121 ggcctgtgcg gaggcgctgc cctagggtaa tgacgccaga tcggcggcgg gtacggtggc
    24181 agtccagcca ccatcgctcg ctacgctggc agtgtaattt ttcccgccaa aggtgacggt
    24241 gacggtttga ccgggttcta cgttagtggt ggtgccggaa agggttaaat cagcgccttt
    24301 ttctgcggcg ttaatcacgt catccgtggc gatggtgttg atggtaatcg ccgctggcat
    24361 gagatcgacc gttaccggat gggtaatact caccggattt ccggcgctac tttccccgga
    24421 tacagcaata gacaccgtcc ccgcaggcca ggcgctaacc tgcgccgccg ttacgcccac
    24481 gctccagcta ccatcggcct gcaccgcagt gatgtattcc acatggttaa tcgtaacggt
    24541 aagcggcgtg ctttcagcca gccccgagct gctgccggta atcaccagcg cctgcccatg
    24601 ctcgatgata ttgaccacat catcgcccgc caccgtatcg acccgcaggc cgggcagatt
    24661 ggcgtcgatg gtgatatccc gcgtgccgct gctggtgttg ccgttttgat tggtgactga
    24721 ggcactcacc gttaaatcgc cattgcccag cgcctgaata tctgccgccg gaacgctcac
    24781 gctccagctt aaaccggcca gcacggtggt ggtgtaggta gtaccgccaa gcgtaacggt
    24841 taccgtgtcg ccaacggcgg ctccagtcac ctgcccgctg atgatttgcg ccacgcccgc
    24901 ttcggcggcg ttaacaatat cgtcgctcgc caccgggtta atggtcacgc cgggcagcgc
    24961 gctgtcgacc agcacgttat ggctggcagt ggcgctgttg ccgatactgc tggttaccgc
    25021 cgccgttacc gtgtagttgg cctggcctaa tgctgtcacc gccgatgccg gaacggtgac
    25081 gctccagttg ccggaggcat cggtcgtcgc cgcatagttt tgcccgttca gcgtcacggt
    25141 gatggctgta cccgctggct gatcgctggt accggtaatc tggagatcgg cccctttttc
    25201 gctggcatta atcacatcat cgcccgcaat gctgttaatg ccgataaggg gcgctgtggt
    25261 attgaccgtg acagtcagtg actggctgcc cgtgtttccg gctttgtcgg ttaccgagac
    25321 gctgacagga taactgccgt ccgccagccc actgaccacc gaggccggaa cgcccagact
    25381 ccaattgccg gaaccatcca ccaccgtggt gtaatccaca ttattcagcg tcacggtgac
    25441 gatatcgccc gcctgagcgc cagtgaccgt gccgctgata atttgcgcct ggatatgttc
    25501 cggcgtgtta atgacgttat cggtcgccac cggattaatg gttaccaccg gcggcgtggc
    25561 atccaccaga gccacgcgcg aaacgctgct cggattaccg gcgacatcgt tcacgctggc
    25621 ggtgaccgta gcgttgccgt cagccagtgc gcccacgttg gcggcgggca cagtcacgct
    25681 ccaggtgccg tctgactgca cgacgccctg atacgtctgc ccattcagcg tgacggtcac
    25741 cgtctgcctt ggctgagcgt cggtggtgcc agtgatttgc aaatcctgcc cgctttcggc
    25801 ggcgttgatg atgttatcgg cggtcagtgg atcgatgctt aaggccgggg cctggctatc
    25861 gacggtaatg gtgcgggaag tgttgccggt attgccgctg agatccgtca cagaagcggt
    25921 aatcgccacc gcgccgtcag gcagatttcc catcgcggcg gcaggtaccg tcagactcca
    25981 ggagccatcg gcctgcacgg tagtggtaaa actctgtcca gccagggtta ctgtgacggt
    26041 ttgtcctgtc tcagcctgtt gagtgctacc ggtaagggtc agtggtgcac ctttttctgc
    26101 gccactgata atgtcatcgc cggagacggt gttaatggtg atcaacggag aaacaccgac
    26161 cagcgtgatt gctcgagacg ccgcgccagt atttcctgca gcatctgtca cggtgacgaa
    26221 aatcgtattg gctccgcgat ccagcgtgcg ggttaccgcc gggtccaacg ccacattcca
    26281 gctgccatct gccagcacga tgcccgtcag gacatgagtg cccaatttca ccgttaccgt
    26341 atcgtcagga gaagcaccgc taacttgtcc ggagatgatc tgcgcgacgg cctgctcggc
    26401 attattaaga atatcgtcgc ccgccacggt attaacactg accacgggtg ctgaggtgtc
    26461 taccgtgaaa ttcgcgctgt ggctggtggt gttccctgcc cgatcgctga cattggcatt
    26521 gacagcgtaa ttaccctccg ccagtgcctg gacttccgtg gcaggcagcg tcacttgcca
    26581 ggttccgtcg ctaccgacgg ttgctgtatg gcttttcccg ttgacggtca gtgttacggt
    26641 ttgccccgct tctgcattcg acgtgccgct cagtaccagc gcgacgttat gttccgcagc
    26701 actgatgatg ttgtcctgag cgatggtgtt gatggcaagc gttggcggtt gggtatcgac
    26761 cgttagcagc tgcgcgccgg tgaccgtatt gcctgtggtg tctttcccac tgaccagtac
    26821 cgcctgattc ccttcgccta acgcctgtgc atcggcggtc ggtactggta cgctccaggc
    26881 accgttagcg cctacgctgg tggtgtaggt tttgccgtta agtgtgacgg tgagttctgt
    26941 tccctgtgcc agatggctac tggtgccaga aagcgtaaac ccggcggcct gttcgctggc
    27001 gttaataatg ttatcgcctg cggtggtggc aacctgcacg ctggcctgac tattgtcgat
    27061 ggttaacgtt atcgaaccgg cgtcagtgat ggtactgccg acaatttgca ccactgacca
    27121 ggtatgttca cctgcacttt gcgtgggtaa cgagacactc cagtgaccgt tgttgtctac
    27181 ctgagtactg gcaatagtat tgccgttact gtctttaatc tgaatagtcg cccccgcctg
    27241 cccgctgccg ctaaaggttg gcgtggtgtc atcggtgatg tcattggtgg cgagaatgcc
    27301 gcgctgatct ccctgattat cggtaactaa aaatgatggt gtggcgttgc caggctcagc
    27361 gggtggcgta gggttattaa tcacttccgt tttcgagtcg ccatcaccac cgcttgccag
    27421 cagtgcacca agcgcaccgc cacctaccgc cgcccccgcc agccaacccc acgggaaggc
    27481 gctggtctga gaaacggtat caagaaatgg cgcaatgctt tcaatcgcag tggtctgggc
    27541 agtcagttct acgggggcta atccacccgc agcagtatcg gcaaaggtga catgggttag
    27601 ctgttgccca tcggcaaaca ccagttccga ttgttcagct gtattcgccg cttgcaggaa
    27661 ataaccgttg cagcggatca ccgtgccgtc ctgcatatag atcagcaggt catttccttc
    27721 acggacgtaa cgcgcaacgg cagaggcctg agcatgaacc tgcaccacgg aaggctcata
    27781 aataacaacc gtgacatcgc caggattaat cagcgtttgt ttgacggatg ttttacgcga
    27841 aataacatca ataatcaggc tcataatggc attcctgata aatggcgcac atccttagcg
    27901 agtccattta tctaaataag acaaaggtaa tcacttaaca acggcccact attaataatg
    27961 gcaggattat ttcaagtcgg gtaagtcgtt gtataagccc tgtttaattc ccgctaatgg
    28021 aataaggtta ttcaccgcag cggcataatt tacggcggcg acccagccat cataattggc
    28081 atttatttca gccgattgcg cctgaaagac atcttgctcg acggttaata gatcattcag
    28141 actgcgttta cccaatttat attcattttg gtacacatct cgcgttttgt gcgcactttc
    28201 ggattgcgct aaaccggctt cttcgcgacc ccgtgcgccg gtccagttcg catatgccac
    28261 agacgctcgt tgcagcacat ccagttttgc ctgttcgacc tgcgaggcag agattttttg
    28321 ttgcccttcg gcctgttgca cctgggcaga aaccgcgccg ccctgataca gcggcgcgtt
    28381 aacgttcagt tgtagctgat catcccaata cgagcggtcg ctggtctggt agcgcgtttt
    28441 acccccctga atactgagcg ttggccagta ttgcgctttc gttttttcca cgccgtactg
    28501 tgctgactgg cgtaagtttt ctgccgccag caccagcggg atggactggt aatcaatatt
    28561 cttcagcgat accggctgct cggctaattc agcaggtggc gcggctatcg cctccggctg
    28621 tacgccagtg agcaccgcca gttgcgcttt ggcgctcgcc atctgcgcct gatattgctc
    28681 cagcgttgag cgcattccag caatacgcgt ctgggcctgt aattcatccg acgatgagtt
    28741 caggccagcg ttagcgcgca atgccgccat gttgtagacg ttttccagcg agtgaatatt
    28801 gcgttgagcc gcatcgcaca aagcctgata acgactgact tccatatagg cagtcgccgt
    28861 tttctctgca acatcggtta aggttgccat caatttaaag cggtagctgt cacgggcggc
    28921 agtttgcaga ttgatatcgt tgttagtttt accaaagtca tacaccagtt gtgtcagggt
    28981 tatgccatac gaaacgttgt tatcaagcct gccgctggag tccgtttgtc gggacggccc
    29041 cgcgttgccg gttaatccaa cgtgcggata ccaggcactt ttggcttcat caatttgcgc
    29101 ttcgccaatg cctatctgcg ccgcctgttg agtgacgtcg ggatttcgcg caaaggcgcg
    29161 taatatactt tcctgtaaag ataattgggc aactggcgtt accgctgggg cattcgccca
    29221 ttgtggcagc ggtgcagcaa ttgcagcttc tcccatcacc ataaaactta taaaaaaaat
    29281 aagtccatta tttttacggt ttttaaactg gaatcgcgcc atagataagt cacttccttc
    29341 caggtcatta ttttaactgg tttttataac cagtctcatt ttattaagaa gggtaataac
    29401 ttgtgcatct cttttgtcga tcattgatat g
//
LOCUS       DAZUGK010000036        29168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZUGK010000036 DAZUGK010000000
VERSION     DAZUGK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.036"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..593
                     /locus_tag="ACHY5W_001421"
     CDS             27..593
                     /locus_tag="ACHY5W_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4446348.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            643..3102
                     /locus_tag="ACHY5W_001422"
     CDS             643..3102
                     /locus_tag="ACHY5W_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4446349.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVAITVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDRRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQLTVHWGDQKT
                     CAIHLPEHLEDVAKRLILPCH"
     gene            3117..3848
                     /locus_tag="ACHY5W_001423"
     CDS             3117..3848
                     /locus_tag="ACHY5W_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4446350.1"
                     /translation="MTFVKGFPLILLVASLCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALLPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSHQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHY5W_001424"
     CDS             3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHY5W_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4446351.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTASGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            5055..6101
                     /gene="abrB"
                     /locus_tag="ACHY5W_001425"
     CDS             5055..6101
                     /gene="abrB"
                     /locus_tag="ACHY5W_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4446352.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(6098..6889)
                     /gene="nei"
                     /locus_tag="ACHY5W_001426"
     CDS             complement(6098..6889)
                     /gene="nei"
                     /locus_tag="ACHY5W_001426"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFS4446353.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6925..7659)
                     /gene="pxpA"
                     /locus_tag="ACHY5W_001427"
     CDS             complement(6925..7659)
                     /gene="pxpA"
                     /locus_tag="ACHY5W_001427"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFS4446354.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            complement(7649..8581)
                     /gene="pxpC"
                     /locus_tag="ACHY5W_001428"
     CDS             complement(7649..8581)
                     /gene="pxpC"
                     /locus_tag="ACHY5W_001428"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFS4446355.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8575..9231)
                     /gene="pxpB"
                     /locus_tag="ACHY5W_001429"
     CDS             complement(8575..9231)
                     /gene="pxpB"
                     /locus_tag="ACHY5W_001429"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFS4446356.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVATHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9254..9997)
                     /gene="ybgI"
                     /locus_tag="ACHY5W_001430"
     CDS             complement(9254..9997)
                     /gene="ybgI"
                     /locus_tag="ACHY5W_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFS4446357.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10268..11749
                     /gene="dtpD"
                     /locus_tag="ACHY5W_001431"
     CDS             10268..11749
                     /gene="dtpD"
                     /locus_tag="ACHY5W_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFS4446358.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11899..13317)
                     /gene="phrB"
                     /locus_tag="ACHY5W_001432"
     CDS             complement(11899..13317)
                     /gene="phrB"
                     /locus_tag="ACHY5W_001432"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFS4446359.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIIKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPPITLNYPRQSFDTAHFPVEEKAAIAQLRLFCQN
                     GAWQYEQQRDFPAVEGTSRLSASLATGGLSPRQCLYRLLAEQPQALEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQRNPAHFKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13314..13823)
                     /gene="ybgA"
                     /locus_tag="ACHY5W_001433"
     CDS             complement(13314..13823)
                     /gene="ybgA"
                     /locus_tag="ACHY5W_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HFS4446360.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDFYNQYRQRVIVLLSHPANRRDHTNVLIHVQGY
                     FRPHIDSAERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPNAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13924..14130)
                     /gene="ybfA"
                     /locus_tag="ACHY5W_001434"
     CDS             complement(13924..14130)
                     /gene="ybfA"
                     /locus_tag="ACHY5W_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFS4446361.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            14442..14531
                     /gene="kdpF"
                     /locus_tag="ACHY5W_001435"
     CDS             14442..14531
                     /gene="kdpF"
                     /locus_tag="ACHY5W_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFS4446362.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            14531..16204
                     /gene="kdpA"
                     /locus_tag="ACHY5W_001436"
     CDS             14531..16204
                     /gene="kdpA"
                     /locus_tag="ACHY5W_001436"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFS4446363.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLLGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            16227..18275
                     /gene="kdpB"
                     /locus_tag="ACHY5W_001437"
     CDS             16227..18275
                     /gene="kdpB"
                     /locus_tag="ACHY5W_001437"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFS4446364.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVSADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            18284..18856
                     /gene="kdpC"
                     /locus_tag="ACHY5W_001438"
     CDS             18284..18856
                     /gene="kdpC"
                     /locus_tag="ACHY5W_001438"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFS4446365.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTELGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASMSVPVELVTASASGLGNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            18849..21533
                     /gene="kdpD"
                     /locus_tag="ACHY5W_001439"
     CDS             18849..21533
                     /gene="kdpD"
                     /locus_tag="ACHY5W_001439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFS4446366.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVTLFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            21530..22122
                     /locus_tag="ACHY5W_001440"
                     /pseudo
     CDS             21530..22122
                     /locus_tag="ACHY5W_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(22285..23925)
                     /gene="pgm"
                     /locus_tag="ACHY5W_001441"
     CDS             complement(22285..23925)
                     /gene="pgm"
                     /locus_tag="ACHY5W_001441"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFS4446367.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGAKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(23951..24496)
                     /gene="seqA"
                     /locus_tag="ACHY5W_001442"
     CDS             complement(23951..24496)
                     /gene="seqA"
                     /locus_tag="ACHY5W_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFS4446368.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            24681..25445
                     /gene="ybfF"
                     /locus_tag="ACHY5W_001443"
     CDS             24681..25445
                     /gene="ybfF"
                     /locus_tag="ACHY5W_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4446369.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            25585..25878
                     /gene="ybfE"
                     /locus_tag="ACHY5W_001444"
     CDS             25585..25878
                     /gene="ybfE"
                     /locus_tag="ACHY5W_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFS4446370.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            26018..26548
                     /gene="fldA"
                     /locus_tag="ACHY5W_001445"
     CDS             26018..26548
                     /gene="fldA"
                     /locus_tag="ACHY5W_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFS4446371.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            26758..26844
                     /gene="uof"
                     /locus_tag="ACHY5W_001446"
     CDS             26758..26844
                     /gene="uof"
                     /locus_tag="ACHY5W_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFS4446372.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            26837..27283
                     /gene="fur"
                     /locus_tag="ACHY5W_001447"
     CDS             26837..27283
                     /gene="fur"
                     /locus_tag="ACHY5W_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFS4446373.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(27367..27693)
                     /gene="chiQ"
                     /locus_tag="ACHY5W_001448"
     CDS             complement(27367..27693)
                     /gene="chiQ"
                     /locus_tag="ACHY5W_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFS4446374.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(27743..29149)
                     /gene="chiP"
                     /locus_tag="ACHY5W_001449"
     CDS             complement(27743..29149)
                     /gene="chiP"
                     /locus_tag="ACHY5W_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFS4446375.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         6696 a         7745 c         7613 g         7114 t
ORIGIN      
        1 tatcatagcc catgaaggat tcatgtatgt ttaaaggaca aaaaacactg gccgccctgg
       61 ccatatctct actattcgct tcacctgttt ttgctgcaga tgaaggatct ggtgaaatcc
      121 actttaaagg tgaagttatt gaagcacctt gtgaaattca ccctgatgac cttgatatga
      181 acattgacct gggtgaagta accacatccc acataaatcg agaacatcat agcgagaaaa
      241 aacctgtcaa tattcgctta atcaactgtg atattgcagg ctgggatgat ggcaacggtg
      301 gcgtggtctc aaaggtaggc gtgaccttcg acagcacggc taaaacgaca ggtgcaacgc
      361 cgctccttag taacatcagt gctggcgagg ctaccggcgt tggcgtacgt ctcatgaaca
      421 aagatgaatc atttgttgta ttgggaacag aagcgccaac aattgactta ttggcaagtt
      481 caacagaaca aactttaaac ttctttgcct ggatggaaca gatggataac gcagtttcgg
      541 ttaccgcggg ggccgttact gctaacgcaa cttacgttct ggattaccaa taatcctttt
      601 taaggcggct gattagccgc cttttgcgaa tattgttcag gtatggatgc cgtgaatatt
      661 tatcgactct ctgttctttc ctgtctggca atggtaaccc ctcctgcgct ggcagctgaa
      721 tttaacctta acgttctcga taagtcgatt cgggatagcg ttgatatttc gttgctcaat
      781 caaaaaggcg tcgtagctcc cggcgattac tttgttgcta ttacggttaa taataataaa
      841 atcagtaatg ggcaacaaat tcgctggcaa aaatctggcg ataaaattat tccttgtatc
      901 aatgaatcgc tgatcgaact cttcggactt aaatccgact ttcgtaaaaa attaccggca
      961 ataaaagaat gtgtcgattt tagtgtcttc cctgaaatca tctttacttt cgatcaggca
     1021 aatcaacagc tcaatattac aatcccgcag gcatggctgg cgtggcattc agaaaactgg
     1081 acgccaccct caacgtggaa taacggtatt cctggcttcc tgatggatta caacctgttt
     1141 gccagcactt atcgcccaca aagcggcagc agcagcaaca acctgaacgc ctatggcacc
     1201 acgggcctca acgccggggc atggcgtctg cgcagcgatt atcagcttag ccagtccgat
     1261 agcggcgaca accgtgaaca atccggggcg atttcacgga cctacctgtt ccgcccgtta
     1321 ccgcaaatag gttcccgatt aaccctgggc gaaaccgatt ttagctctaa catttttgat
     1381 ggtttctcct ataccggtgc ggcgttagcc agcgacgatc gaatgctccc ctgggagtta
     1441 cgcggctacg cgccgcaaat cagcggcatc gcacagacta acgccacggt cacgataagc
     1501 cattctggtc gcgtgattta ccagaaaaaa gttccgccag gcccatttat tattgatgac
     1561 ctgaatcagt cagtgcaagg tacactggat gtcaaagtga gcgaagaaga tagacgggtg
     1621 aataacttcc aggtttcagc ggcctctacc ccgttcctga cgcgtcaggg ccaggtgcgc
     1681 tataaactgg ctgccggtcg cccgcgttcc tctatgtcgc accacaccga ggatgaaacc
     1741 ttcattagcc atgaagtctc ctggggaatg ctctccaaca cttcactata tggcggcatg
     1801 cttctcgcag gcgatgacta tcgttccggc gcgctgggta ttgggcaaaa tatgctctgg
     1861 ctgggggcgc tgtcgtttga cgtgacctgg gccgacagcc attttgatac tcaacaggat
     1921 gagcagggtt acagctaccg ctttaactac agcaaacagg ttgatgcgac caacagcacc
     1981 atctcattgg cagcctatcg cttctccgat cgccattttc acagctacgc taactacatt
     2041 gatcacaaat acaatgacgc cgacgcgcag gatgaaaagc aaaccatcag cctctccttc
     2101 ggtcagccta ttactccgct gaatcttaat ctctacgcca acatcctgca tcagagctgg
     2161 tggaatgcgg atacctccac cacggccaac attaccgccg gttttaacgt cgacattggc
     2221 gactggaaag atatttctgt ctcgacctcg ttcaacacca cccattatga agataaagat
     2281 cgcgacaacc agatttactt ttcgatctcc ttaccgattg gggaaagtgg tcgactgggt
     2341 tatgacatgc agaacaacag taatactacc acccaccgca tgtcgtggaa cgataccctg
     2401 gatgaacgaa acagttgggg aatgtcggct ggaatacaat ccgatcgccc ggataacggt
     2461 gcgcaggtca gcggcaatta ccaacacctc agttcggcgg gggaatggga tctgaccgga
     2521 acctatgccg ccaatgatta cacttccgcc agtgccagtt ggagcggctc gtttaccgcg
     2581 actcaacatg gcgcggcatt ccatcgccgc agttccacca atgaaccacg cctgatggtc
     2641 agcaccgacg gcgtgggcga tattccgatt cagggcaata ttgactacac caaccgcttt
     2701 ggtattgccg tggtaccgtt tgtttccagc taccagccta cgacggtggc ggtcaatatg
     2761 aacgacttgc ctgacggcgt taccgtttcc gaaaacgtgg tgaaagaaac ctggaccgaa
     2821 ggcgccatcg gctttaaatc gctggcgtcc cgcgccggga aagatctcaa cgtcattatc
     2881 agcgatgcta acgggcattt cccgccgctc ggtgccgatg ttcgccaggc tgaaggcggt
     2941 gtcagcgtcg ggatggttgg cgaaaacggt cacgcatggc tgagtggggt cgatgaaaat
     3001 caacaactta ccgtgcactg gggcgaccag aaaacgtgtg ccattcatct gccggaacat
     3061 ctggaagatg tggctaagcg cctgatttta ccctgtcatt aattaaggaa aagacgatga
     3121 catttgttaa aggattccct ttgattcttc tggttgccag tctgtgcagc cacggcgcag
     3181 tgcagcctga ccgcacgcgc atcatcttta atagcaaaga taaagcaacc agcctgcgcg
     3241 tggaaaaccg cagcgataaa ctcccctacc tcgcctattc atggatagaa aatgaaaagg
     3301 gagagaaaag cgatgaattt ctcgtcgccc tgctccctat ccagcgactg gagccaaaag
     3361 cgacttcgca ggttcggatc atgaaacaag ccgccaccgc gaaattaccc accgatcgcg
     3421 aatctctctt ttactataac ctgcgtgaaa ttccgcctgt cccggaaggc agcgagggcc
     3481 atgcgatcct gcaagtcgcc gtccaaagtc gcattaagct attctggcgg cccgccgcat
     3541 tacgcaaaaa aatgggcgat catgttgagc aacagctaca ggttagccat cagaataacc
     3601 agcttaccct gaaaaacccg accggatact atctgactat tgcctatctt gggcgggatg
     3661 agaaaggcgt gctgcctggc tttaaaagca cgatggtcgc accgttcagt tcggtcacca
     3721 ccagcacggg cagctatagc ggcaagcagt tttatcttgg ctacatggac gactacggcg
     3781 cattgcgtat gaacacgctc tcctgccagc ggcaatgccg cctgcagccg gtggagaata
     3841 aaaaatgaga ttcgccaaag gcgtgttgct cgccatctgt ctgatttttt tgccgctaaa
     3901 agcggcgctg gcgctgaact gctattttgg taccgcaagc ggcgctgtag aaaaatcgga
     3961 agctattatg cccttcgccg ttccggccaa ttccaaaccc ggcgataaaa tctgggaatc
     4021 tgacgatata aaaattccgg tctactgtga caacaacaca aacggtaatt ttgaaagcga
     4081 gcacgtctac gcctgggtaa acccttaccc cggtattcag gatccctatt atcaattggg
     4141 cgtaacctat gaaggcgtgg actatgacgc aagccttggg aaaagccgca tcgacacgaa
     4201 tcagtgcatc gacagtaaga atattgatat ttacaccccg gagcagatca ttgcgatggg
     4261 ctggcaaaat aaactttgct ccggcgatcc aaccgttatg cacaaatcgc ggacgtttgt
     4321 ggcgcgtatg cggctttacg ttaaagttcg cgccatgccg ccccatgatt atcagagcaa
     4381 actcagcgat tacatcgtcg tacagtttga tggcgcaggc agcgtgaacg aagaccctac
     4441 cgcgaagaat ctgaaatatc acattaccgg tctggaaaat atccgcgtgc tggattgcag
     4501 cgtcaacttt gctatctcac ctgaaaccca ggtcgttgat tttggccgat ttaacgtgct
     4561 ggatattcgt cgccatacga tgtcgcagca atttaaaata actacaacca aatcacaaaa
     4621 cgaccagtgt accgacggtt ttaaagtcag ctcttcgttt tataccgatg aaacccttat
     4681 cgacgaagat aaatcactgc tcattggcaa tggcctgaag cttcgattgt tggatgaaaa
     4741 tgcctcaccc tatactttca acaaatacag tgagtatgcc gattttacca gcgacctgtt
     4801 agtgtatgaa aaaacctaca cggcagaatt gtcatccacg ccgggtacgc ccatcgacgt
     4861 tggtcccttt gatacagtgg tacttttcaa aattaactac aactgattta gcctattgcc
     4921 ctttccagcc ggagagggct tcttcggtta attgccaatt ttccggcttt tattgattac
     4981 cgttattttt tccatgcccg gcattcttta tgctgtatcg atacaccaaa gagaaaataa
     5041 tgagggagca aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta tcacttctcc
     5101 tttctattgg tttcctcgcg gtacatctcc cggcggcatt attacttggg ccgatgatcg
     5161 ccgggatcat cttcagtatg cgcggggtta ccctgcaact cccccgctcc gcttttctcg
     5221 ccgcgcaggc cattcttggc tgcatgattg cgcaaaactt gaccggttcg atcctgacca
     5281 cgctggcggt taactggccg attgtgttag ctattttact ggtgacgttg ctttccagcg
     5341 ctatcgtggg atggctactg gtgcgctata gctcactgcc cggaaatact ggtgcctggg
     5401 gctcctcgcc tggcggggct gcggcaatgg tcgcgatggc gcaggattac ggcgcagata
     5461 tccgcctggt ggcgtttatg cagtatctgc gagtgctgtt tgtcgcgggg gccgcagttc
     5521 tggtcacgcg aatgatgctg ggcgataacg ctgaagcggt taaccagcat atcgtctggt
     5581 tcccgccagt gagcattaat ctcctgctta ctattttgct cgcggtcgtt gccggtacag
     5641 tgggatgcct gctgcgtctt ccttccggca cgatgctcat cccgatgctg gcgggcgcag
     5701 tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg gcgatggcgt
     5761 atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta ctgcgggcac
     5821 tacgcccgct accacaaatc ctgctgtcga tttttgctct gctggctatt tgtgcgggta
     5881 tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac ctcgccacca
     5941 gccccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc
     6001 tcattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacaggg cctgccattg
     6061 cgcggtttat ttcaacctat gcgccgaaac gtacggccta gtgctggcag cccggacacc
     6121 agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gccgccacag cgttcgcaca
     6181 gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc ttattctcat
     6241 ccacctgccc ccgcgtagcg taagaaaacc gaggaatatc cagtaacgcg tgtgcgagtg
     6301 catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac ccaacctgcc
     6361 agaggatctc cacacgcaaa taattgccaa gcccagccag aaacgcctgc tcgagcagta
     6421 atccagcaaa ctgacggtta cgaaagcgcg gcgacaataa ccgttctttc accacctccg
     6481 gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga tgcgtggtca
     6541 gttgttccgg ggtcaacatc tcaatatccg aggcgctata aagcagaatg gttttgtcag
     6601 ccgtttgcag ttttacccgc aatactcgcg tggtctgcga ctcttcgccg gtatcaacca
     6661 cgcgccagac gccataaagc tgattatggc tgtagagcgt taagtcgttg gaaaaatgag
     6721 ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata agttgtgatt
     6781 gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg cctttgatcg
     6841 ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg gtgatatcct
     6901 tttttcattc tttattaatc ggggttatgc tgcgacaaca atcccctttt cggcaaatgc
     6961 agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca ggcagaccgt
     7021 ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt gttgcaccat
     7081 ctccaacgtt tgcgccagcg cctgttcttc gttttcaatc aacgcgcctg actggcttcg
     7141 cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct cgcgcgttgt
     7201 caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa cgagaaccaa
     7261 tgctggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg cttctttcgc
     7321 cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc cttgcgcacg
     7381 ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg tttctggcgg
     7441 cagttgcata gcgctgcgac caaaattttc cctgtcggga aaactcggat gagcgccaat
     7501 cgcgacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg catcgcctgc
     7561 atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg cgtcgctggt
     7621 gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc cacgctaatt
     7681 gttcgaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt gaacactgga
     7741 caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca gcctcaatga
     7801 tacaggcaat acgtggataa ccaccggtgg tctgtgcgtc gttcatcaac acaatgggct
     7861 gcccgttatg cggcacctgc accacgcccg gtaacaaacc gtgagataac agttcgcgat
     7921 cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta ctttgcgagc
     7981 taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt
     8041 ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg ccctgcgctt
     8101 ccataaaatc acgcttcgct ttgccaatcg ggagtcggtc accatccttc agtaaacggc
     8161 cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt accggcggaa
     8221 catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg cgtttaagcg
     8281 ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca ttatcatcca
     8341 gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg aactcaaccg
     8401 ttaactgacc gagcgtgatc tccagcgcgg gggcattggc atcattaccc accagcaggt
     8461 tagcaatgcg taacgcaggc atatccagtg cgccgcagtg gctgataccc gactggcgaa
     8521 aaccgtgacg accgccatct tgcacagtgg tatacatgcc cgcacgaata atcttcagca
     8581 tactccctcc ttttgcggta caaagcgcac gctgtctccc ggacgtaata agatgggttc
     8641 gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc agccacccgg
     8701 cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac ctgccggaac
     8761 gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac tcccgagata
     8821 cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg agtgcaattc
     8881 aacaacctgt ttttcgctca acccgcaatg cgtcgcgacc accgccaaat ccggtccgcc
     8941 tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca gggcctcgct
     9001 ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact caggattacg
     9061 caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg gcatatccac
     9121 cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg gcggttccag
     9181 ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca ctttcgatcc
     9241 ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca agatcggtat
     9301 tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca tggtgacctg
     9361 cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc
     9421 cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt tgcccgccgc
     9481 ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac cataacggct
     9541 tacgtcccag acgcgcttca atccaggaag ccagctccag tcccggcaca ggcatggtca
     9601 gttcgcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc agtaacgccg
     9661 ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc cagccataca
     9721 ggttgatatc attcgccagc aacgttttta aacggttacg cttcatgccg cgaatgaccg
     9781 gagattcgcc tttccagaag tagccatgat gcacaatgac cgcatccgcg cccagacgca
     9841 ctgcctcatc gagcaaagcc tggctggcag tgacaccggt aacaattttt tgcaccgtct
     9901 ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt
     9961 tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc gtattctgat
    10021 agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc ttttgacata
    10081 ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg cttcagctaa
    10141 tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta tcaaagtttt
    10201 aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg tcaattgtgg
    10261 aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg ttgcgctgca
    10321 aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct atctcaccaa
    10381 tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact gttcgctggt
    10441 gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca atcgcatggc
    10501 ggtgatgctg ggggcgttat tgatggcgat cggtcatgtg gtgttgggtg ccagtgagat
    10561 ccatccgtca ttcctctatc tgtccctggc gataatcgtt tgtggctatg gtctgtttaa
    10621 atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc gtcgtgatgg
    10681 cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac ctattgcctg
    10741 tggctacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg cggttggcat
    10801 gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata cccgcggcgt
    10861 taacaaaaaa gtactgcgtg cgacaaactt tctgctgccg aactggggat ggctgctggt
    10921 tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagaat ggtcggtata
    10981 cgccttgatt gtcgcgacta tcattggcct gggtgtactg gcaaaaattt atcgcaaagc
    11041 agaaaaccag aaacagcgga aggagctggg gctgattgtg acgctcacct tcttcagtat
    11101 gttgttctgg gccttcgcac aacagggcgg tagctcgatt agcctttata tcgaccgctt
    11161 cgttaaccgc gatatgtttg gttataccgt tccgaccgcg atgttccagt cgattaatgc
    11221 cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gtaaaagaga gtgtcgcggg
    11281 taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga tgagcgccgg
    11341 attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact cttctctgcc
    11401 actgatggta ttaggcctgg cggtgatggg ctttgcggaa ctgtttatcg acccggttgc
    11461 tatgtcgcag attacgcgca ttgaaatccc cggtgtgacc ggcgtattaa ccggcattta
    11521 tatgctgctt tccggcgcga ttgcgaacta tctggcgggc gtgattgccg atcagacatc
    11581 gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat atattgaagt
    11641 atttgatcaa attacctggg gcgcactggc gtgtgtagga gtggtactga tgatttggct
    11701 gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcttaac aggccagcac
    11761 cgatgcctga tgcgacgctt gacgcgtctt atcagaccta cgtatttcct gcaatttatt
    11821 gaatttgtac aaatttgtag gctggataag gcgttcacgc cgcatccggc atctgggctc
    11881 gattgcctga cgcgtctgtt atttcccctt ccgcgccgcc tcatacgctg ccaacgtttg
    11941 tactctcgcc tctttgtgct cgactatcgg ttgcggataa tccagcttca cacctgcttt
    12001 ctgcgcccac ttccacggct catgcaccga tttccccggt acattgcgta gttccggtag
    12061 ccactggcgg ataaactcgc cttcgcggtc aaatttctcg ccctgggttg tcgggttgaa
    12121 aatacgaaaa tacggcgctg catcagtacc cgtagaagcc gcccactgcc agccaccgtt
    12181 attggctgcc aaatcaccat caatcagctg cgacatgaaa tatcgctcgc cttcgcgcca
    12241 gtcgatcaac aaatctttca ccagaaaact ggctgtaatc atccgtagcc tgttatgcat
    12301 ccagccagtg ctgttaagct gacgcatagc tgcatcaaca atcgggtatc ccgtttttcc
    12361 ttcctgccag gctttaaaat gggcgggatt gcgctgccac tgcacacgat ccgtccaggc
    12421 aataaacggg cggtgtttac acagcgaagg ataatacgtc atcaaatggc ggtagaactc
    12481 gcgccatatc agctcattaa gccagacgct acccggtccg ccttccagcg cctgcggttg
    12541 ttccgccaac aggcgataca aacactggcg aggcgataag ccgcccgttg ccaggctggc
    12601 agataaacgg ctggtgcctt ctaccgccgg gaaatcacgc tgttgctcgt actgccaggc
    12661 accgttctgg caaaaaaggc gtaattgcgc aatcgccgct ttttcttcca ccgggaaatg
    12721 tgcagtgtcg aaagactgac gaggataatt cagcgtaata ggtggagcgg gatctatcga
    12781 tccgctgcta cgaacttttg gcgcggcgac gcactccggc atcccttccc gcagccgttt
    12841 cagccaggca ttcttaaaag gcgtaaagac tttgtacatc tcatgattgc cggtcatcac
    12901 cgcgccaggc ggcaggatca cgctgtcatc aaatccttca cacaccacgt tacgcagcgc
    12961 tctttcaact tccacatccc gcgcccgctc attcacctca tactgatagt tataaaaaag
    13021 atgagtaacg ctgttttccg cgcacacctg tttaattatt tcgacgctgg cggcaaagtc
    13081 atccacttca cggaacaata aaggaatacc tttttccgcc agcgcgattt gtagcccgtt
    13141 cagttgagca ttgataagtt cagcctgacg cggcgacata ttatgcgccg cccactggcg
    13201 tggtgtagcg atatacaacg ccagcacgcg tgcagatgaa ttgcggcagg cggcagccag
    13261 cgcgagatta tcatgcagac gtaaatcctg gcgaaaccag accagatggg tagtcataaa
    13321 actcctgaat ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg ctgccctgaa
    13381 agccaggcat taggataaag cgccatatag tgtttgatac gcatcagcgg cgcaagaagc
    13441 ggttgctcgc cacgacgata actgtcgata agcgccgcca gctgctgacg ttctgctgaa
    13501 tcaatatgcg ggcgaaaata accctgaacg tgtatcaaaa cattagtgtg gtcacgccgg
    13561 ttggcggggt gagaaagcaa aacaattacg cgttggcggt actggttgta aaaatcatcc
    13621 agattttgcc accggtgaat atcagccacg aacgggccaa gtttgcggta ctccggctga
    13681 gaatgcgcca gaaagacgag tttatagcgg ctgtgataat cgagaagcgc cccgcgcgtt
    13741 aagccatgtt ctttcagtcg atgtaactca tgaagcgcga agatacggat tagagtgctg
    13801 tcatcaaatc gttgctgatt cattactgct cctgaacggt ttacaggagt aagtgtagac
    13861 ggcgaaaaga aaaaaccgcc agcgtttcca ctagcggttt tcaatgtatt caaaactcag
    13921 caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccaaaccg gtttatcgct
    13981 ggttttcgac cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag
    14041 catgaaaggc agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag
    14101 ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta tctacccggt
    14161 gaatggcacc ggaaaatgaa tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat
    14221 tttactactc atccgaccac ttatttttgc ttattgatgg tttatttaca ttcaccctgt
    14281 aattaagcta cacaaaagtt aaattaatac taaacattag ttaaatcatg gcttttacca
    14341 tttttatact ttttttacac cccgtccgca gatttttgcg aaatctttgc agccagaatt
    14401 ctatccttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag
    14461 ccggcgtgtt gctggtgttt ttattactgg gttatctggt ttatgccctg atcaatgcgg
    14521 aggcattctg atggctgcgc aagggttctt actgattgcc acgtttttac tggtattaat
    14581 ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc
    14641 aggtacagcg ggcgttgaac gtatactctt tcgtttactt ggcgtctctg accatgagat
    14701 gaactggaag caatatcttt gtgccattct cggcctgaac atgctggggc tggcggtgct
    14761 gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccggggct
    14821 gtcgtgggat ctggcgctca ataccgccgt cagctttgtc acgaatacca actggcaatc
    14881 ttatagcggt gaaaccacgt tgagctattt cagccagatg gcgggcttaa cggtgcaaaa
    14941 ctttctttct gccgccagcg ggattgcggt gatttttgcc ttcatccgtg catttacccg
    15001 ccagagtatg agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt
    15061 gctagtccct gtggcgttgt tgatcgcact gttttttatt caacaaggtg cgctgcaaaa
    15121 ctttctgccc tatcaggcag tgaataccgt tgaaggagcg caacagctgt taccaatggg
    15181 tccggtggct tctcaggaag cgatcaagat gctcggcact aacggcggtg gtttctttaa
    15241 tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct
    15301 ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg
    15361 ccgccagggg cgcatgttgc tgtgggcgat gtcggtgatt tttgtcatct gcgtaggcgt
    15421 ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg gcgctgggtg cggacagcag
    15481 catcaatatg gaaggtaaag agagccgttt cggcgtgttg gtcagtagcc tgtttgcggt
    15541 cgtgactacg gcggcgtcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct
    15601 cggtggcatg gtgccgatgt ggctgatgca aattggtgaa gtggtattcg gcggtgtcgg
    15661 ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat
    15721 gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgtgaga tgaaactgac
    15781 tgcactggcg attctggtca ccccgacgct ggtgctgatg ggcgcggcgc tggcaatgat
    15841 gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta gcgaagtgct
    15901 gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa
    15961 ctctccgttc tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggagtgat
    16021 tatcccggtg atggcaattg ccggttcgct ggtaagtaaa aagagccaac ccgccagctc
    16081 cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct
    16141 ggttggcgca ctaaccttta tccctgccct ggcgcttggt ccggtggcgg aatatctctc
    16201 ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa
    16261 cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc
    16321 gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg
    16381 cgatggcaag cggcgcgatg cctggcaatg cgctgtttag tgcggcaatt agcggctggc
    16441 tgtggatcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag
    16501 cgcaggccaa cagtctgaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc
    16561 cgaaatatgg cgctgcggcg gacaaagttt ctgccgatca acttcgtaaa ggcgatatcg
    16621 tactggtaga agctggcgat attatcccct gcgatggtga agttattgaa gggggtgcat
    16681 cggtcgatga aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg
    16741 attttgcctc cgtcaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta
    16801 gcgttaaccc cggcgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc
    16861 gacgcaaaac gccgaacgag attgccctga ccatcctgct gattgccctg actatcgtct
    16921 ttttactggc aaccgccacg ctgtggccgt tttctgcgtg gggcggtaat gcagtcagtg
    16981 taacggtact ggtggcgctg ctggtctgtc tgatcccaac cactattggc ggcctgttgt
    17041 cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg
    17101 gacgtgcagt tgaagcggcg ggtgacgttg acgtcctgct gctggataaa accggcacca
    17161 tcacactcgg taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa
    17221 cgctggctga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggccgcagta
    17281 ttgtgatcct cgccaagcag cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg
    17341 ccacctttgt accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca
    17401 tgatccgtaa aggttctgtc gatgccattc gccgccatgt tgaggctaac ggtggtcact
    17461 tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg tcagggggcc acaccgctgg
    17521 tagtagtaga aggttctcgt gtgctgggcg ttattgcgtt gaaagatatc gtcaaaggcg
    17581 gcattaaaga acgcttcgcc caactgcgca aaatgggtat taaaacggtg atgattaccg
    17641 gcgataaccg tctgactgcc gccgccattg ccgcggaagc gggcgtcgat gattttctcg
    17701 ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggccgtt
    17761 tggtagcgat gaccggcgac ggcaccaacg atgctccggc gctggcgcag gcagatgtcg
    17821 cggtggcgat gaactctggt actcaggcgg cgaaagaagc gggcaatatg gtggatctcg
    17881 actctaaccc gaccaagttg atcgaggtgg tgcatattgg caaacagatg ctgatgaccc
    17941 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc
    18001 cggcggcgtt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg cgcctgcatt
    18061 cgcccgactc cgccattctc agtgcggtga ttttcaacgc cttgattatc gtctttttga
    18121 ttcccctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc
    18181 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt atcaaagtca
    18241 ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt accatgagtg gattacgtcc
    18301 ggcattatcg acatttatct ttctgttatt gattactggc ggcgtttacc cgctgctgac
    18361 caccgagctg gggcaatggt ggtttccctg gcaggccaac ggttcgttga tccgtgaagg
    18421 tgatacggtg cgcggttcgg cattaatcgg gcagaatttt accgacaacg gctattttca
    18481 gggtcgcccg tcggcaacgg cagaaatgcc ttataatcca caggcttctg gcgggagcaa
    18541 tctggcggtc agtaaccctg agctggataa actaatagcc gcacgcgttg ctgcattacg
    18601 ggccgctaac ccgaatgcca gcatgagcgt tccggttgaa ctggtgacgg cgtcggcaag
    18661 cgggctgggc aataacatca ccccgcaagc ggcggcctgg cagatccctc gcgtagcgaa
    18721 agcgcgtaat ctcagcgttg aacagctcac gcaactgatc gcaaaataca gccaacaacc
    18781 gctggtgaaa tatatcggcc agccggtcgt caacattgtt gaactcaatc tggcgctgga
    18841 taaacttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
    18901 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgcgcaggc gtcgggaaga
    18961 cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
    19021 gcgtggtaga aacccacggg cggaaagata ccgccgccat gctggaaggg ctggctgttc
    19081 tgccgccaaa acgcctggcg taccgtgggc ggcacatcag cgagtttgat ctcgatgccg
    19141 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgccag
    19201 gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct ggcattgatg
    19261 ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca
    19321 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
    19381 tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
    19441 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
    19501 tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc
    19561 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
    19621 gccataacac cggcagcgaa aaactggtcc gcgcggcggc gcggctggcg tcacggctgg
    19681 ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa
    19741 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
    19801 cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg
    19861 gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acgtttgctg
    19921 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
    19981 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
    20041 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta attgccatgc
    20101 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctactt ggcgtggtgg
    20161 tggtgacgct attttatgga cgctggcctt cggtggttgc taccgtcatt aatgtggtga
    20221 gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc
    20281 tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt actgcgggtg
    20341 tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac ttatatgaaa
    20401 tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc agcgaacaat
    20461 ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat
    20521 tgcagccgtt aacacatccg caaggaatga cgccgtggga cgatgccatc gcgcagtgga
    20581 gttttgataa aggcctgcct gcgggtgcgg gcaccgacac gttacccggc gtgccgtacc
    20641 agattttgcc gttaaaaagc ggtgagaaaa cctacgggct ggtggtggtg gaaccgggga
    20701 atctgcgtca gttgatgatc ccggaacagc agcgcctgct ggagacgttt acgctgttag
    20761 tcgccaatgc gcttgagcgg ctgacgctaa ccgccagcga agaacaggcg cggatggcaa
    20821 gtgaacgtga acagatccgc aacgctctgc tggcggcgct ttcgcacgat ttacgcactc
    20881 cgctcacggt gttattcggt caggcagaaa tcttaacgct cgatctggca agcgaaggat
    20941 cgcctcacgc ccgccaggcc agcgagatcc gtcagcatgt gctgaacacg acccgactgg
    21001 tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt
    21061 ggttaacgct ggaagaagtg gtcggcagtg cgctgcaaat gctggaaccg ggtttatcgt
    21121 cgcccatcaa tctttctctg ccagagccgc tgaccttaat ccacgttgac gggccacttt
    21181 ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg
    21241 aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc tgggataacg
    21301 gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata
    21361 aagagtcggc agtaccgggg gtagggcttg gactggcaat ttgtcgggcg atagtggatg
    21421 tacacggggg cactattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta
    21481 cacttcccca gcaaactgcc cctgaacttg aagaatttca tgaggatatg tgacaaacgt
    21541 tctgattgtt gaagatgaac aagctattcg tcgctttctg cgcacggcgc tggagggcga
    21601 cggtatgcgc gtctttgagg ccgaaacgct gcaacgcggc ttgctggaag cggcaacccg
    21661 taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga ttgagtttat
    21721 ccgcgacctg cgccagtgga gcgcggtgcc ggtgatagtg ctttccgcac gcagcgaaga
    21781 gagcgacaaa atcgccgcca ttcagccact gccgcgcccg atccgctggt gaaattttcc
    21841 gatgttaccg tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc
    21901 acgccgattg aattccgcct gctggcggtg ctacttaaca atgccggaaa agtactcacc
    21961 cagcgccagc tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcattatttg
    22021 cgtatttata tgggacatct gcgacaaaaa ctggaacagg atcccgctcg cccccgccat
    22081 ttcattactg aaaccggtat tgggtatcgg tttatgcttt aggcggttaa atgctggatc
    22141 cggcgtgaac gccttatccg gcctacaaaa tcgcttaaat tcaatatatt gcaccatcct
    22201 cgcaggcctg ataagcatag cgcatcaggc agtcataagt aaaaaagggc gatcttgcga
    22261 ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac
    22321 cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt
    22381 atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt
    22441 cagaccgcca atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc
    22501 acctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg
    22561 tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag
    22621 ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccaacag
    22681 acacatgatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga
    22741 agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa
    22801 ccatttgaaa cctaccggga cttctaccag tttacgaccc aagtcgttga ccacacggtc
    22861 gatcatcgca gatgaaacca gcgttttacc gacggcaaca tctttgcccc actgcggacg
    22921 atgctggaac aggtaattga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc
    22981 cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt
    23041 atcacgcagt gccagcaggc ctgccattgc acactcagag gagcagtcca tacggatcgc
    23101 gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatagt
    23161 caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg aaccgcccag
    23221 cggatcaacg ccaagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc
    23281 cagcccttcc acaaacggct gcaccaaatc ctgctctttc acatgaccgg atgccatcgc
    23341 ttcgtcgagg gagatacgct tcgcgccttt caggccatcg gccatcagtg cgttggctct
    23401 gtcttccacc actttagtga cgttggtatc tgccgggcca ccatttggcg gattgtattt
    23461 gattccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg
    23521 gccacctttt ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gggtgaagcc
    23581 attgttttcc tgcacaataa catctacgcc gttcgctgcc agcacttcca gaacggaaat
    23641 aaatgcaggt tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc
    23701 gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa
    23761 gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc
    23821 attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa
    23881 atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt
    23941 ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg
    24001 actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc
    24061 aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt
    24121 gttcatctgc cgcaaagtaa acgcgcgtac gaccgtgcaa cgattccgtt gcttcggcaa
    24181 acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc
    24241 gcttttgctc tgcgtattca tccgaaagca gaagttcacg cattgcgcga accttgtctt
    24301 taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg
    24361 tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg
    24421 atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa
    24481 cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaggag
    24541 tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat
    24601 aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac
    24661 gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca caaaaccagc
    24721 acaataattc tcccatcgtt cttgtccatg gtctgtttgg cagcctcgac aaccttggcg
    24781 tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg
    24841 gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata
    24901 ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag
    24961 cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg gcgatcgata
    25021 tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttctcggct atcaacgcgg
    25081 tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta
    25141 atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta
    25201 acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat
    25261 gggatcaccc tgccctgttt atccctggtg gtaattctcc gtatgttagc gagcagtacc
    25321 gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc
    25381 actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg
    25441 attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg
    25501 tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt
    25561 gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat
    25621 ctgttcgcgc acgagcgtcg accgggacga ccgaaaacaa atccgctttc gcgcgatgaa
    25681 cagctgcgta ttaataaacg caaccaacta aaacgcgaca aagtacgtgg ccttaagcgt
    25741 gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggaatcgcgc
    25801 aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt
    25861 agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt
    25921 gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca
    25981 cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt
    26041 cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa
    26101 agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttatga
    26161 cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga
    26221 cttcttcccg actctcgaag agattgattt caatggcaaa ctggttgcgc tgtttggttg
    26281 tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat
    26341 tattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga
    26401 agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg
    26461 tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt
    26521 gcatctcgac gaaattctca atgcctgatg tgatgcggcg tagactcatg tctacgccgt
    26581 attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc
    26641 tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt
    26701 ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg
    26761 atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta
    26821 acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt
    26881 aacgcttcca cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag
    26941 tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt
    27001 atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg
    27061 cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga
    27121 ctgcggcaag gttatcgaat ttagtgatga ttcaatcgaa gcgcgtcagc gtgaaattgc
    27181 cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga
    27241 aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagtc tgaacgagaa
    27301 aagccaacct gcgggttggc ttttttatgc aagggaaata agaaaagcga gagttacagc
    27361 tctcacttat ttgttattac tgcgaatttc ctgccagacc ttatcacaat cggctttcac
    27421 cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcggac
    27481 actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct
    27541 ctggcaggct tcaatttttt ccggcgaacc ttgtgcggtg ttgatacagg cgctgtatgc
    27601 ctctttcaga cggctgtctt cctgtggtgc ggttgattgc gcacaagcta ccagccccga
    27661 tgccatcatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg tgcggcaggc
    27721 acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca
    27781 cgtcacgctc atcctggaag atgttaccgt aaccgccgcc ccagcttggg atgtcggagt
    27841 ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa
    27901 tggtgtagac cgcatccagg ctgtatgcag actcttcaat agtccggttt ttgtcgtagt
    27961 acgcatccgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag
    28021 cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca ccgaagaaga
    28081 ccgctttttc gccgttggcg ttgaagtcag aacggttatc ccaccagata tccaggcgac
    28141 cgttcgagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc tgaccgtcag
    28201 ctttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg
    28261 tcaacgcttg cagccaggcg gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt
    28321 tatcgcgcgt accgtagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt
    28381 tgtagctggc tttggcaaag tattgatcga tatacccttc cgcctgacca aatgccgctt
    28441 ccagtacgaa gttatttttg aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt
    28501 tggtagtttt atcgttctga taaaactcat ccatttccag atgccacggt gctttgtatt
    28561 cgttggtcca catgtaggag aaactcaacg caccggcatc gccgtaatca aaattcgctc
    28621 cggcttccgc accctgatag gtacctggca taaagctcca gtgcggcgct aacagcgtct
    28681 gaccggttgg ctgaatataa cctgccctcg cccaaaccgg accgtattta aatttggccg
    28741 ctgctttata caggcttata ccgcttttat cgccggacca gtcttcgtca taggctttat
    28801 tactttttga aaacgcgatt tcgttcgggt gagagctgtc gccgttttcc gccatttcaa
    28861 tcgccgtaaa cgcggcaata tcaaggccga acatatcagc agcataacca gactgaaaat
    28921 cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa
    28981 catctttacg gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt
    29041 cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac
    29101 cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct
    29161 ctttgacg
//
LOCUS       DAZUGK010000037        28588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZUGK010000037 DAZUGK010000000
VERSION     DAZUGK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.037"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..2396
                     /gene="fepA"
                     /locus_tag="ACHY5W_001450"
     CDS             156..2396
                     /gene="fepA"
                     /locus_tag="ACHY5W_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFS4446376.1"
                     /translation="MNKKIHSLALLVNLGIYGIAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTALYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            2571..3191
                     /gene="entD"
                     /locus_tag="ACHY5W_001451"
     CDS             2571..3191
                     /gene="entD"
                     /locus_tag="ACHY5W_001451"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFS4446377.1"
                     /translation="MKTTHTALPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3313..3465)
                     /gene="hokE"
                     /locus_tag="ACHY5W_001452"
     CDS             complement(3313..3465)
                     /gene="hokE"
                     /locus_tag="ACHY5W_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFS4446378.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGESLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(3816..3968)
                     /gene="hokE"
                     /locus_tag="ACHY5W_001453"
     CDS             complement(3816..3968)
                     /gene="hokE"
                     /locus_tag="ACHY5W_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFS4446379.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            3937..4205
                     /locus_tag="ACHY5W_001454"
                     /pseudo
     CDS             3937..4205
                     /locus_tag="ACHY5W_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4155..4346)
                     /locus_tag="ACHY5W_001455"
     CDS             complement(4155..4346)
                     /locus_tag="ACHY5W_001455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446380.1"
                     /translation="MFLFFGDGELMVPEAIWLLLQCDKLWQSWLMAKNFDDWGAQNKT
                     GSASVDQMRSEKSETRLTV"
     gene            4421..5539
                     /gene="ybdK"
                     /locus_tag="ACHY5W_001456"
     CDS             4421..5539
                     /gene="ybdK"
                     /locus_tag="ACHY5W_001456"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFS4446381.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVGNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVVTDPHTGDRRPLKEDTLRLLEKIAPSAHKIGASSAIEAL
                     NRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            5605..5853
                     /gene="ybdJ"
                     /locus_tag="ACHY5W_001457"
     CDS             5605..5853
                     /gene="ybdJ"
                     /locus_tag="ACHY5W_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFS4446382.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            5918..6286
                     /gene="ybdF"
                     /locus_tag="ACHY5W_001458"
     CDS             5918..6286
                     /gene="ybdF"
                     /locus_tag="ACHY5W_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4446383.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            6380..7033
                     /gene="nfsB"
                     /locus_tag="ACHY5W_001459"
     CDS             6380..7033
                     /gene="nfsB"
                     /locus_tag="ACHY5W_001459"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFS4446384.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            7141..8388
                     /gene="ybdG"
                     /locus_tag="ACHY5W_001460"
     CDS             7141..8388
                     /gene="ybdG"
                     /locus_tag="ACHY5W_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFS4446385.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(8456..9832)
                     /gene="pheP"
                     /locus_tag="ACHY5W_001461"
     CDS             complement(8456..9832)
                     /gene="pheP"
                     /locus_tag="ACHY5W_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFS4446386.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     REAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9934..13077)
                     /gene="cusA"
                     /locus_tag="ACHY5W_001462"
     CDS             complement(9934..13077)
                     /gene="cusA"
                     /locus_tag="ACHY5W_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFS4446387.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLGEVKNALDASNQEAG
                     GSSIELAEVEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVIWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGEMVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVNVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(13089..14312)
                     /gene="cusB"
                     /locus_tag="ACHY5W_001463"
     CDS             complement(13089..14312)
                     /gene="cusB"
                     /locus_tag="ACHY5W_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFS4446388.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(14328..14660)
                     /gene="cusF"
                     /locus_tag="ACHY5W_001464"
     CDS             complement(14328..14660)
                     /gene="cusF"
                     /locus_tag="ACHY5W_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFS4446389.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14684..16066)
                     /gene="cusC"
                     /locus_tag="ACHY5W_001465"
     CDS             complement(14684..16066)
                     /gene="cusC"
                     /locus_tag="ACHY5W_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFS4446390.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            16223..16906
                     /gene="cusR"
                     /locus_tag="ACHY5W_001466"
     CDS             16223..16906
                     /gene="cusR"
                     /locus_tag="ACHY5W_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFS4446391.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16896..18338
                     /gene="cusS"
                     /locus_tag="ACHY5W_001467"
     CDS             16896..18338
                     /gene="cusS"
                     /locus_tag="ACHY5W_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFS4446392.1"
                     /translation="MVNKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLMD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLVDEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            18488..20725
                     /gene="nrfB"
                     /locus_tag="ACHY5W_001468"
     CDS             18488..20725
                     /gene="nrfB"
                     /locus_tag="ACHY5W_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFS4446393.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            20712..23684
                     /gene="nfrA"
                     /locus_tag="ACHY5W_001469"
     CDS             20712..23684
                     /gene="nfrA"
                     /locus_tag="ACHY5W_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFS4446394.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPIQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADFRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            23685..24575
                     /gene="ybcH"
                     /locus_tag="ACHY5W_001470"
     CDS             23685..24575
                     /gene="ybcH"
                     /locus_tag="ACHY5W_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFS4446395.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNEQRLISDVSAKPVYISSFFAGNMSTDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            24758..25519
                     /gene="envY"
                     /locus_tag="ACHY5W_001471"
                     /pseudo
     CDS             24758..25519
                     /gene="envY"
                     /locus_tag="ACHY5W_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            26032..26985
                     /gene="ompT"
                     /locus_tag="ACHY5W_001472"
     CDS             26032..26985
                     /gene="ompT"
                     /locus_tag="ACHY5W_001472"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFS4446396.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            27172..>28588
                     /locus_tag="ACHY5W_001473"
     CDS             27172..>28588
                     /locus_tag="ACHY5W_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4446397.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVH"
BASE COUNT         7239 a         7455 c         7100 g         6794 t
ORIGIN      
        1 gcgtaatggc gcgccgtggg aagcgcggac attaattaac caactgcact gcgtgtcttt
       61 caggatcaaa ggttttcgcg gtagcgggat gcgtcgtgtt gatgacgacc atgcccgaca
      121 gttgcaattc gtggcaaaaa tgcaggaata aaacaatgaa caagaagatt cattccctgg
      181 ccttgttggt caatctgggg atttatggga tagcgcaggc acaagagccg accgatactc
      241 ctgtttcaca tgacgatact attgtcgtta ccgccgccga gcaaaactta caggcgcctg
      301 gcgtttcgac catcaccgca gatgaaatcc gcaaaaaccc cgttgcccgc gatgtgtcgg
      361 agatcatccg taccatgcca ggcgttaacc tgaccggtaa ctccaccagt ggtcagcgag
      421 ggaataaccg acagattgat attcgcggta tgggtccgga aaacacgctg attttgattg
      481 acggcaagcc ggtaagcagc cgtaactcgg tgcgtcaggg ctggcgtggc gagcgcgata
      541 cccgtggtga tacctcctgg gtgccacctg aaatgattga acgtattgaa gttctgcgtg
      601 gtccggcagc tgcgcgttat ggcaacggcg cggcgggcgg cgtggttaac atcattacca
      661 aaaaaggcag cggcgagtgg cacggctcct gggacgcata tttcaatgcg ccagaacata
      721 aagaggaagg tgccaccaaa cgcaccaact tcagtctgac cggtccgctg ggcgacgaat
      781 tcagcttccg cttgtatggc aacctcgaca aaacccaggc tgacgcgtgg gatatcaacc
      841 agggtcatca gtccgcgcgt gccggaacgt atgccacgac gttaccagcc gggcgcgaag
      901 gggtgatcaa caaagatatt aatggcgtgg tgcgctggga cttcgcgcct ctgcaatcac
      961 tcgaactgga agcgggttac agccgccagg gtaacctgta tgcgggcgat acccagaaca
     1021 ccaactctga cgcttacact cgctcgaaat atggcgatga aaccaaccgc ctgtatcgcc
     1081 agaactactc gctgacctgg aacggtggct gggataacgg cgtgaccacc agcaactggg
     1141 tgcagtacga acacacccgt aactcgcgta ttccggaagg tctggcgggc ggtaccgaag
     1201 ggaaatttaa cgaaaaagcg acacaggatt tcgtcgatat cgatctcgat gacgtgatgc
     1261 tgcatagcga agttaacctg ccgattgatt tcctcgttaa ccagacgctg acgctgggta
     1321 cggagtggaa tcagcaacgg atgaaggact taagttccaa cacccaggca ctgaccggaa
     1381 cgaataccgg tggcgctatt gatggcgtga gtgccaccga ccgtagcccg tattcaaaag
     1441 cagaaatttt ctcgctgttt gccgaaaaca acatggagct gactgacagc accatcgtaa
     1501 cgccggggct gcgtttcgat catcacagta ttgtcggcaa taactggagc ccggcgctga
     1561 acatatcgca aggtttaggc gatgacttca cgctgaaaat gggcatcgcc cgcgcctata
     1621 aagcgccgag cctgtaccag actaacccaa actacattct ctacagtaaa ggtcagggct
     1681 gctatgccag cgcgggcggc tgctatctgc aaggtaacga tgacctgaaa gcagaaacca
     1741 gcatcaacaa agagattggt ctggagttca aacgcgacgg ctggctggcg ggcatcacct
     1801 ggttccgtaa cgattatcgc aataagattg aagcaggcta tgttgctgta gggcaaaacg
     1861 cagtcggcac cgctctctat cagtgggata acgtgccgaa agcggtggtt gaaggtctgg
     1921 aaggatcgtt aaacgtaccg gttagcgaaa cggtgatgtg gactaataac atcacttata
     1981 tgctgaaaag tgaaaacaaa accacgggcg accgtttgtc gatcatccca gagtatacgt
     2041 tgaactcaac gctgagctgg caggcacgag aagatttgtc gatgcaaacg accttcacct
     2101 ggtacggtaa gcagcagccg aagaagtaca actataaagg tcagccagcg gttggaccgg
     2161 aaaccaaaga aattagtcct tacagcattg ttgggctgag cgcgacgtgg gatgtgacga
     2221 agaatgtcag tctgaccggc ggcgtggaca atctgttcga caaacgtttg tggcgtgcgg
     2281 gtaatgccca gaccacgggc gatttggcag gggccaacta tatcgccggt gccggggcgt
     2341 atacctataa cgagccggga cgtacgtggt atatgagcgt aaacactcac ttctgatgct
     2401 aacgtcagat tgttgacaaa gtgcgcgtcg ttcatgccgg atgcggcgtg aacgccttat
     2461 ccggcctaca aaaatcttgc caattcaata tattgcagga ccatgtaggc ctgataagcg
     2521 tagcgcatca ggcagttttg cgtttgtcat cagtctcgga tatggtcgat atgaaaacta
     2581 cgcataccgc cctccccttt gccggacata cgctgcattt tgttgagttc gatccggcga
     2641 atttttgtga gcaggattta ctctggctgc cgcactacgc acaactgcaa cacgctggac
     2701 gtaaacgtaa aacagagcat ttagccggac ggatcgctgc tgtttatgcc ttgcgggaat
     2761 atggctataa atgtgtgccc gcaatcggcg agctacgcca acctgtgtgg cctgcggagg
     2821 tatacggcag tattagccac tgtgggacta cggcattagc cgtggtatct cgtcaaccga
     2881 ttggcattga tatagaagaa attttttctg cgcaaacagc gaccgaattg acagacaaca
     2941 ttattacacc agcagaacac gagcgactcg cagactgcgg tttagccttt tctctggcgc
     3001 tgacactggc attttccgcc aaagagagcg catttaaggc aagtgagatc caaactgatg
     3061 caggttttct ggactatcag ataattagct ggaataaaca gcaggtcatc attcatcgtg
     3121 agaatgagat gtttgctgtg cactggcaga taaaagaaaa aatagtcata actctgtgcc
     3181 aacacgatta attgacaaca tctggtacga ttcgcccgca gccatcactg actgcgggcg
     3241 aaagtgtaaa gcaggtgcct taccactctg acctgacaac cggatatgcg gggattgctc
     3301 cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact caatattacg
     3361 ttcctttacg gtgaactcac acagcgactc tccgaccaga agcgtaaatc ccagcaccgt
     3421 caaacacagc actatgactg ccacaagggc atatttcgtc agcatgttta tattgcctcc
     3481 atgcgaagaa gggactacca tccgagttgt tgaggctcgg agtggcagcc ccaggattga
     3541 tagaaatatc tcctggggct tcatctttcc agacctcaga gttaacctga aaccggaaag
     3601 cttcaggcac ccgccgttat cttacccttc cttagcaaaa tggctgttta tttatacagt
     3661 gagtcctgtt tcggacttct gtcggcaaaa ctcatcattg ctcttaaaac gaaatcgccc
     3721 gcagccatca ctgaccgcgg gcgaaagtgt aaagcaggtg ccttaccatc ctgacctgac
     3781 aaccggatat gcggggatta ctccccgcac aacggctact tcttcggttc gtaagcgaga
     3841 acagccctga actcaatatt acgttccttt accgtaaact cacacagcga gtctccgacc
     3901 agaagcgtaa atcccagcac tgtcaaacac agcactatga ctgccacaag ggcatatttc
     3961 gtcagcatgt ttatattgcc tccatgcgaa gaagggacta ccatccaaat tgttcaggtt
     4021 tgggtaggta gccccagggt tgatagaaat atctcctggg gcttcatctt tccagacctc
     4081 agagttaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc ttcctcaata
     4141 aaatagctgt ttatttatac agtgagtctt gtttcggact tctcactcct catttgatca
     4201 actgaagctg agccagtttt attctgcgcg ccccaatcat caaaattctt cgccatcagc
     4261 cagctctgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagg caccatcagt
     4321 tcaccatcac caaaaaaaag aaacattcca gaacttccgc atctcgccca gccgctccga
     4381 cttctatact gaatagaaaa cgccaacata agagaaacct atgccattac ccgattttca
     4441 tgtttctgaa ccttttaccc tcggtattga actggaaatg caggtggtta atccgccggg
     4501 ctatgactta agccaggact cttcaatgct gattgacgcg gttaaaaata agatcacggc
     4561 cggagaggta aagcacgata tcaccgaaag tatgctggag ctggcgacgg atgtttgccg
     4621 tgatatcaac caggctgccg ggcaattttc agcgatgcag aaagtcgtgt tgcaggcagc
     4681 cgcagaccat catctggaaa tttgcggcgg tggcacgcac ccgtttcaga aatggcagcg
     4741 tcaggaggta tgcgacaacg aacgctatca acgaacgctg gaaaactttg gctatctcat
     4801 ccagcaggcg accgtttttg gtcagcatgt ccatgttggc tgtgccagtg gcgatgacgc
     4861 catttatttg ctgcacggct tgtcacggtt tgtgccgcac tttatcgccc tttccgccgc
     4921 gtcgccatat atgcagggaa cggatacgcg ttttgcctcc tcacgaccaa atattttttc
     4981 cgcctttccc gataatggcc cgatgccgtg ggtcggtaac tggcaacaat ttgaagccct
     5041 gtttcgctgt ctgagttaca ccacgatgat cgacagcatt aaagatctgc actgggatat
     5101 tcgccccagt cctcattttg gcacggtgga ggttcgggtg atggataccc cgttaaccct
     5161 tagccacgcg gtaaatatgg cgggattaat tcaggccacc gcccactggt tactgacgga
     5221 acgcccgttt aaacatcagg agaaagatta cctgctgtat aaattcaacc gtttccaggc
     5281 ctgccgctat gggctggaag gcgtcgttac cgatccgcat actggcgatc gtcgaccgct
     5341 aaaggaagac accttgcgat tgctggaaaa aatcgcccct tccgcacata aaattggtgc
     5401 atcgagcgcg attgaggccc tgaatcgcca ggtcgtcagc ggtctgaatg aagcgcagct
     5461 gatgcgcgat ttcgtcgccg atggcggctc gctgattggg ctggtgaaaa agcattgtga
     5521 gatctgggcc ggtgactaag tcgggaattg ccttacagaa gaatatccct gacaatggtg
     5581 ttttaactta catttaacaa tgttatgaaa caccctttag aaaccttgac caccgcagca
     5641 ggcattttgc tgatggcttt cctctcttgc ctgctgcttc ccgcccccgc attggggctg
     5701 acgctggcac aaaaactggt gaccatgttc catctgatgg atcttagtca gctttacact
     5761 ttattgtttt gtctgtggtt tttagtgctg ggcgctattg agtattttgt tctgcgcttt
     5821 atctggcgac gctggttctc gctggcggat taaacgtcga ttatcgccga acttcgcggc
     5881 atactttgct tattctcctt cgcctaaagg aacgtttatg gataagcaat cactgcacga
     5941 aacggcgaag cgcctggctc ttgagttacc ctttgtcgag ctttgctggc cttttggccc
     6001 ggagttcgat gtttttaaaa ttggcggcaa gatttttatg ctgtcgtcgg agctacgcgg
     6061 cgtccccttt atcaatctga agtccgatcc acaaaaatcc ctgttaaatc agcaaatata
     6121 cccaagcatt aagccagggt atcacatgaa taaaaagcac tggatttcgg tgtatcccgg
     6181 cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac gattcgtgga atctggtggt
     6241 tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca ggctaaagcg gaaatctata
     6301 gcgcattttt ctcgcttacc atttctcgtt gaaccttgta atctgctggc acgcaaaatt
     6361 acttacacat ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc attccactaa
     6421 ggcatttgat gccagcaaaa aacttacccc ggaacaggcc gagcagatca aaactctgct
     6481 gcagtacagc ccatccagca ccaactccca gccgtggcat tttattgttg ccagcacgga
     6541 agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat tatgtgttca acgaacgtaa
     6601 aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg acgatgcctg
     6661 gctgaagctg gttgttgacc aggaagatgc cgatggccgc tttgccacgc cggaagcgaa
     6721 agccgcgaac gataaaggtc gcaagttctt cgccgatatg caccgtaaag atctgcatga
     6781 tgatgcagag tggatggcaa aacaggttta tctcaacgtc ggtaacttcc tgctcggcgt
     6841 ggcggctctg ggtctggacg cggtacccat cgaaggtttt gacgccgcca tcctcgatgc
     6901 agaatttggt ctgaaagaga aaggctacac cagtctggtg gttgttccgg taggtcatca
     6961 cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt ctgccgcaaa acatcacctt
     7021 aaccgaagtg taattctctc ttgccgggca tctgcccggc tatttctcat cagattttac
     7081 ttatttgcat aacccccttt cagccgtcat cataggctgc tgttgtataa aggagacgtt
     7141 atgcaggatt taatatccca ggttgaagat ttagcgggta ttgagatcga tcacaccacc
     7201 tcgatggtga tgattttcgg tattattttt ctgaccgccg tcgtggtgca tattattttg
     7261 cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg gctttggttg
     7321 caaatcatta cccagaataa actcttccac cgtttagctt ttaccctgca ggggattatc
     7381 gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga tattctgact
     7441 acctgcgcgc agttgtggat catgatgtat gcgctgcttt cagtcttctc gttgctggat
     7501 gttattttga atctggcgca gaaattcccg gcagcatccc agttaccgct gaaagggata
     7561 tttcagggga ttaaactgat cggcgcgatt ctggtcggca ttttgatgat ctcgctgctg
     7621 attggtcagt cgccagcgat tctgatcagc ggtcttggtg caatggctgc cgtgctgatg
     7681 ttggtattta aagatccgat tcttggtctg gtggcaggta ttcagctttc cgcgaacgat
     7741 atgctgaaac tgggcgattg gctggagatg ccgaaatacg gcgcggatgg cgcggtgatc
     7801 gatattgggt taaccaccgt caaggtgcgt aactgggaca ataccattac cactattccc
     7861 acctggtctc tggtttctga ctcctttaaa aactggagcg ggatgtcagc atctggcggg
     7921 cgacgtatta agcgcagtat cagtattgat gtcaccagta ttcgttttct tgatgaagac
     7981 gaaatgcaac gtctgaataa agcgcatttg ttaaagcctt atttaaccag ccgccatcag
     8041 gaaattaatg agtggaatcg ccagcaaggt tctacggagt cggtattaaa cctgcgccga
     8101 atgaccaata ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa ccatccgcgg
     8161 attcgtaaag atatgacctt aatggtacgc caactggctc ctggtgataa cggtttaccg
     8221 ctcgagatct atgcgtttac caacaccgtg gtgtggctgg aatatgaaag cattcaggct
     8281 gatatattcg atcacatatt tgcgattgtc gaagagtttg gtctgcgact tcatcagtcg
     8341 ccaaccggca atgatattcg ctctctggcg ggtgcattta agcagtaatt aaaaaaaccg
     8401 ctctcatcga atggatgaga gcggacatcg ttttgtcgga cgtagcgtga atgccttatt
     8461 tccgacgcag cgttttaaat gctacaaaca ggaatacaat ccacaccggc agcaggatcg
     8521 ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatggcg aggaaggcaa
     8581 tgcaaagata gttgccgaac ggataaagca gcgccttaaa ctgtgtttca cgtccctgac
     8641 gtcgcatcgc ctcgcgaaaa cgcagatgcg ccaggcagat cataatccag ttcaacagca
     8701 gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc agatagttga
     8761 ttaacaccac cagcgaagtg atcgctccgg aaagcatcag cgagttaatc ggcacgccgc
     8821 gacggctgac gcgagtcaaa aacttcggcg cattaccctg tacagaaagg ccaaacagca
     8881 tacgactgtt ggagtaaacc ccgctgttat acaccgacag cgatgctacc agaatgacaa
     8941 agttcagcgc agaagctacc acgttactgt cgagattatg gaaaatcatc acaaacgggc
     9001 tactgttgga tttcacttcc acccacggat agagcgccag taaaaccacc agtgaaccga
     9061 tgtaaaacag caggatgcga tacaccacct gatttaccgc ttttggaatg cttttttccg
     9121 gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag accgccgaag gagaacataa
     9181 ttaccgccag cgacaaaatc agcccattcc agccggtggc gaagaaacca ccgtagcgcc
     9241 agaggttgtc gatactggct ttctcgccgc cgtgaccaga aaacagcagc cacaggccaa
     9301 agccgatcat accgatgatt gccagcactt taatcagcgc aaaccagaac tcggtttcgc
     9361 catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag gcggcagccc
     9421 aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca gcggtcagct
     9481 ctgccattcc caccagcacg aacatcaccc agtagttcca gccggagagg aacccggcga
     9541 acggtcccca gtatttatag gcaaagtggg caaatgaacc ggataccggc tcttcgacca
     9601 ccatctcgcc gagctggcgc ataatcagga aagcgatgat cccggcgacg ccgtagccca
     9661 gcaatacagc cggacccgcc atctgaatcg ccgggccaat gccaagaaac agaccagtac
     9721 caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg cgatgaagcg
     9781 tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc acgcctttcc
     9841 cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc gtcgcaagac
     9901 acaatccaca cggttaaacg gggtatcctg cttttatttc cgtacccgat gtcggtgcag
     9961 ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggcg cggtgatcat
    10021 gccgccaatc atcggcgcgg caatccggct catcacctct gaccctgctc ctgttcccca
    10081 cagaatcggc agcagaccag cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg
    10141 cagtaccgcg ccgtgataca acgcctcatc cagcttctgc tcgctgaatg tttgcggatt
    10201 attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca ccacgccaaa
    10261 ttcggcggcg accccggcga gggcgataaa gccagtgccc gtcgccacag aaagatgaaa
    10321 gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc tgctgataat
    10381 cagcaacgct tcgcccaccc gacggaatgc cagatacaac agcacgaaga tgatcatcaa
    10441 cgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct cgaactgccc
    10501 ggagaacgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg ctttttgcag
    10561 atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg
    10621 gcgcgcattc tcggttttca gcatcgacgg tccggtagag acgttaacgt cggccacgtc
    10681 cgccagggtg atttgctgct tcatcggcgt caggatcggc aactggcgca gcgcctgcgg
    10741 gctatcgcgc cagctttgcg gataacgcag attaatggga taacgggcaa ttccttccac
    10801 catttcgcca accatcgccc cgcccaccgc agatgtcaca aacaattgca catccgccac
    10861 cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat agcgcccacc
    10921 ttccagacgc tcggcaagcg cagaagccac gcctggcacc gttcgcgcca cttcttcaat
    10981 ttgctccgcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa tgccgatggg
    11041 gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca cccacagatt
    11101 cgccagcccc ggcagacgca cggtgttatc cagttcctca atgattttgt ccatcgtcat
    11161 gcctggccgc cactgatcct gtggcttaag ctgaatggtc gtttcaacca tctccagcgg
    11221 agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg ccacttcagg
    11281 tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg ctgcggaaat
    11341 ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc
    11401 cccgccaact ttattgagcg gccagataac cgtcagcacc gaaagcgccg ccaccagcag
    11461 cgtggttttc ggccagtgca gtaccttcag caacagcgga tgataaacac gaatcaaaaa
    11521 gcgattgagc gggttactgc tttctggcgg aattttgcca cggatccagt aacccatcag
    11581 gatagggatc actacgatcg ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa
    11641 cgccagcggg ccaaacagac gcccttcctg cccttccagg gtgaagatcg ggataaacga
    11701 cagcgtgata atcagcagac tgataaacag cgccggcccc acttcaacag atgcatcggt
    11761 aatcacctgc cagcgcgttt tattatccag tgtggcgtca ggatgctggt gctgccactc
    11821 ttccagccgt ttatgtgcat tctcgatcat gacgatagcg gcatcgacca tcgccccgac
    11881 ggcaatcgca atgccaccca gcgacataat attggcattc agcccctgga agtgcatgac
    11941 aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac
    12001 atgccagaga aacagcgcac agactaccgc caccacaata aactcttcca gcaacttgcc
    12061 gctgaggttg tcgatggccc ggtcaatgag ctggctgcga tcgtatgttg taactatctc
    12121 cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg cggcgatcac
    12181 ttctcgggcg tttttgccgg agcgcaggat caccaccccg cccgcaactt cgccttcacc
    12241 gttcagttcg gcaatgcccc ggcgcatctc cgggccaacc tggaccttcg caacatcacg
    12301 cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa agtcgtcgag
    12361 cgtttgcaga tagccgctgg cgcgcaccat atattccact tccgccagtt cgatcgacga
    12421 accgcccgct tcctggtttg aagcatccag cgcatttttc acttcgccga gactgatgcc
    12481 atactgcgcc aggcgctgag gatcgataac cacctgatac tctttcacca caccgcccac
    12541 cgacgccact tccgcaacgt cagggatggt ttttagctca tatttgagaa accagtcctg
    12601 caatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg catattcata
    12661 gatccagcca acacccgtgg catctggccc cagctcggca ctgactcccg caggcagctt
    12721 accctgtacc tggttgaggt actccagcac ccgcgagcgc gcccagtacg gatcggtacc
    12781 atcttcgaaa atgacataca cataagagtc gccaaactgc gagaaaccac gcacagtctt
    12841 cgcgccaggc accgacaaca tggtggtagt tagcggataa gtcacctgat tttcaacgat
    12901 ttgcggtgcc tgaccgggat agctggtttt aataatcacc tgcacatcgg agagatccgg
    12961 caacgcatcc actggcgtgt taatgatggt ccaggtgccc cagatactca gaaataacgc
    13021 gcccatcagc accagaaaac ggttcgccac cgagcgacga ataatccatt caatcattgg
    13081 ttattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag tgcgccagaa
    13141 atattggctt cggaatcaat caggaacagg ccgctggaaa ccaccttttc accttccgcc
    13201 agaccagagc gtaatgcggt gacaccttgc gatgcctgga aaacggcaac gcgtttcggt
    13261 acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt cgctgccggt atcaatcagc
    13321 gcttgtgacg gaatgagcag catcggttcg ctggcggtat tgagttgcaa ccaggcgttc
    13381 attcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg
    13441 gtcgcggcat ccacgccagg tagtagcgtc catttgcgga tggtgagtgt tttatcaggt
    13501 cgcgccggaa cggtgagcgt aaactgcgag gcatctttca ccagccaggc gatggactcc
    13561 gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgctac cacgttatct
    13621 ttggcgatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc aatgggtgct
    13681 ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat atccgcctcc
    13741 ggcattcccg ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc cgtaccgccg
    13801 gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcgggaat ggtcaggtcg
    13861 agaagcggtg tgcccttttg cactttatcg cccacggtaa gcggatacac cttgtcgata
    13921 aacccggcag cgcgagcctg cacaatggca tactgatact cgttgtaact gacattcgcc
    13981 gggaaactct gggcaaaagt cagcggcccg cgcgtgacgg tagccgtttt cacccccaga
    14041 ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc atcggcatat
    14101 ttcggcacca gatccatatc cataaacggc gatttacctg gtttatcgaa ccgcgtattg
    14161 ggatacatcg ggtcgtacca gaataagatt ttacgttctg cggtcgacgt tttttctgcg
    14221 ggtggttcag cctttgcaaa ccaggtaaaa cctgccgcag aaataatacc gcccgcgatc
    14281 atgctgccga taataagcgc gatttttttc atctcattaa acctgggtta ctggctgact
    14341 ttaatatcct gtaataaaga aaggttgccc tgctggacaa aattaaacgc cactttgtcg
    14401 ccagttttaa tttcactcat tttcgtctgc ggagtgatgg taaagcgcat ggtcatctcc
    14461 ggccagttca cggcagcaat cggatcgtga tggatggtga tttttttgct ttccagatca
    14521 acacctttta ccacgccggt agcactaatg acctgtggtt gtgcttcgct catggtttca
    14581 tgatgatgtt cgttagcctg ggcattaaag ccaataacgg taaacagact gaacattgcg
    14641 acttgcagtg cttttttcat ttattctctc ctggagttaa aaattattgc tgccaaccgc
    14701 caccaagtgc ggtatataaa gaaatttcgt tgacctgacg ggcataattc aggtcaagta
    14761 aggtttgtcg ggttgcaaat aaagaacgct cggcatccag cacttccaga taacttactg
    14821 cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgat gccagataac
    14881 gctgctgggc gctaatttga tcgttcaggc tttgacgtaa tgccagcgca tctgccactt
    14941 ctttaaaggc gttctggatt ttctgttcat aattcaccac cgactgctgc tggcgaattt
    15001 cggcgatatc cagattggcc tggttacgtc cggcattaaa aatggggatc tcaattttgg
    15061 gtataaaatt ccacatcccg ctgctggcgt taaataatga cgacagatcg ctgctggcgg
    15121 ttgatattcc gctggtcagg ctgatagacg ggaaaaatgc cgcgcgtgca gcaccaatat
    15181 tggcattagc cgccattaac gcgtgttcag cttccataat atccgggcgc tgcaataaga
    15241 tttgcgacga caagcccgcc ggtaatttaa cgctttgcag actgtcgctg tttactgtct
    15301 gcgcttgcgg cagcttgccg tagcttccca ataacagttg caatgcatta ttcgcctgcg
    15361 ccagttcccc ctgacgttta gcgatgtcac tgcgggtact ttctatcacc ccgcgagcct
    15421 gttccagcgc cagaacattg ctgctaccgg tcaacagttg tttttcgaca aacgcatatg
    15481 actgctgata attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac gccagttgct
    15541 gattgaaata gctttgcgcg acattagaaa ccagcagaat atgcaccgcg cgctgagctt
    15601 cctcagtggc taaataattt tgtcgctcgg cttcgctcat gttctttaag cgcccgaaaa
    15661 aatcgaggtc aaagctggcg ttaaggccag tagaaaactc ccgcgtcgtg gctgaatcgc
    15721 ctttaagatt gccgctccag ctgccgctgc cctcgccatt gagctgtggg tagcggtcgg
    15781 catcggtcag acgatattgc gcccgtgcct cctgcacttt cagcgtcgcc atgcgcaaat
    15841 cccggttatt caccagcgcc tcgctaatca gcgtcttcac ctgattatca acaaaaaagg
    15901 tgcgccagcc cgcgttctga tagttatctg ctgcgttaac gaggccgttc tggctgagtg
    15961 agaactgctg cggtacgggc attgccgggc gctgataatc cggtgccagt gaacaaccgg
    16021 ttagcgcaag ggccacacaa aatggcagaa gtttacaagg agacataggc tcataatttc
    16081 tggtgatttt ataccgccaa ctttactcac caggctctga ttttccggtg acaggaaaat
    16141 gacaaaattg tcattttgcc aataagcgat tgccatctga tcccgctact ctagaattgc
    16201 ccgggcaaca tgtggaggaa atatgaaact gttgattgtc gaagatgaaa agaaaaccgg
    16261 agaatacttg accaaagggt taaccgaagc cggttttgtg gtcgatttgg ccgacaacgg
    16321 gctgaatggc taccatctgg cgatgaccgg tgattatgat ctgataatcc tcgatattat
    16381 gctgccggac gtgaacggct gggatatcgt gcgcatgttg cgctccgcca ataaagggat
    16441 gccgattctg ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg ggctggagtt
    16501 gggggcagat gactacctgg tgaaaccatt cgcttttgct gaactgctgg cgcgggtgcg
    16561 cactctcctg cgacgcgggg cggcggtgat tatagaaagt cagtttcagg ttgccgatct
    16621 gatggtcgat ctcgtcagcc gcaaagtcac tcgcagcggc acgcgcatca ctttgaccag
    16681 taaagagttt actctgctgg agttttttct ccgccatcag ggcgaagtgc tgccccgctc
    16741 gcttatcgcc tcgcaggtat gggacatgaa ttttgacagc gataccaacg ccattgatgt
    16801 ggcagtgaag cggctgcgcg gcaaaatcga caacgacttt gaaccaaagc tgattcaaac
    16861 cgtgcgcggc gtgggttaca tgcttgaggt acctgatggt caataagcca tttcagcgcc
    16921 cgttttcgct ggcaacccgc ctgacctttt ttatcagcct ggccaccatc gcggcgtttt
    16981 tcgcctttgc atggatcatg atccactcag taaaagtgca ttttgccgag caggatatta
    17041 atgatttaaa agagattagt gccacccttg aacgggtact aaatcaccct gacgaaacgc
    17101 aagcccgacg cttaatgacg ctggaagata tcgtcagtgg ttattccaat gtgttgattt
    17161 ccctggcaga tagtcagggt aaaacggtgt atcactcccc cggtgcgccg gatatccgcg
    17221 agtttacgcg tgacgccata cccgataaag acgcgcgggg cggtgaggtg tatctccttt
    17281 ccggcccgac gattatgatg ccaggccacg gtcacgggca tatggaacac agcaactggc
    17341 ggatgattaa cttaccggtt ggcccgctga tggacggcaa accgatttat acgctctaca
    17401 tcgcgctttc tatcgatttt catcttcatt acataaatga tttgatgaat aaacttatta
    17461 tgaccgcatc ggtaatcagc atcctgatcg tctttatcgt gctgttggcg gtacataaag
    17521 gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa tatcacctcg aaagatctcg
    17581 acgttcgcct cgacccgcag accgtgccca ttgagctgga acagctggta ctgtcgttca
    17641 accatatgat cgagcgtatt gaggatgtct ttacccgcca gtccaatttc tcagcggaca
    17701 tcgcccacga aattcgcaca ccgattacca atctcatcac gcaaaccgaa atcgccctca
    17761 gccagtcgcg cagccagaag gagctggaag atgtgctcta ctctaatctc gaagagctga
    17821 cgcggatggc gaaaatggtc agcgatatgc tgtttctcgc tcaggccgat aacaaccagt
    17881 taatccccga aaagaaaatg ctcaacctgg tggatgaagt cggcaaagtg ttcgattttt
    17941 tcgaggcgtt agcggaagat cgcggcgtgg agttgcgatt tgttggcgac gaatgtcagg
    18001 ttgcgggcga tccgctgatg ctgcgtcggg cgttaagcaa cctgctttct aatgccctgc
    18061 gttatacgcc acccggagag gctattgtag tgcgctgcca gacggtcgat catcaggtgc
    18121 aagttatcgt cgaaaacccc ggtacgccca ttgcgcccga gcacttaccg cgattgtttg
    18181 accgtttcta ccgcgttgac ccctcccgcc aacgaaaagg cgaaggtagc ggcattgggc
    18241 tggcgatagt gaaatcaatt gttgtcgcgc ataaaggcac ggttgccgta acgtcagatg
    18301 cgcgggggac aaggtttgtt atcacattac ccgcttaatt attcagcaaa cggcaacttt
    18361 tataaccagt gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt
    18421 cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt taatgtaagg
    18481 tttttgtgtg gactggcttc ttgatgtttt tgctacctgg ctctacggct taaaagtaat
    18541 cgcgataacg ttagcggtca tcatgttcat cagcgggctg gacgattttt ttattgatgt
    18601 cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat
    18661 gagttaccgc gaactgtata aaccagatga aaaaccgtta gcgattatgg ttccggcgtg
    18721 gaatgaaacg ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc tcgactacga
    18781 aaactatcat atatttgttg gcacctaccc caacgacccc gatactcagc gtgatgttga
    18841 cgaagtgtgc gctcgcttcc cgaatgtgca taaggtagtc tgcgcgcgtc ctggccccac
    18901 cagcaaagcc gactgtctga acaacgtgct ggacgccatc acccagtttg aacgtagcgc
    18961 caatttcgct tttgctggtt ttattctgca tgacgccgaa gatgtgattt caccgatgga
    19021 attgcgtctg ttcaactatc tggtcgagcg taaagatctg attcagatcc cagtgtatcc
    19081 gtttgaacgc gaatggacgc acttcaccag catgacttac attgatgagt tttcagagct
    19141 gcatggcaaa gatgttccgg tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt
    19201 tggcacctgt ttcagccgcc gtgccgtgac cgcactgtta gctgacggtg acggtattgc
    19261 tttcgacgtg cagagtctta ctgaagatta cgacattggc ttccgcctga aagaaaaagg
    19321 tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg
    19381 taaattttta cagcacgcgc ggacatcaaa tatgatctgc gtgcgcgaat atttcccgga
    19441 taccttttcg actgcggttc gacaaaaatc tcgctggatc atcggcattg ttttccaggg
    19501 ctttaagacc cataaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg
    19561 caaaggggca atcagtaact ttgtcagctt cctcgcgatg ctggtgatga tccagctttt
    19621 gctgttgctg gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt ctattttcag
    19681 cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggtttgatgg ttaaccgcat
    19741 cgtgcagcgg gtgattttcg ttactggcta ctacggcctg acgcaggggc tgctttccgt
    19801 cctgcgtctt ttctggggca acctgattaa cttcatggcc aactggcgcg cgttaaaaca
    19861 ggtacttcaa cacggcgatc cacgtcgcgt ggcgtgggat aaaacaacgc atgacttccc
    19921 cagcgtcact ggcgataccc gctcgttgcg cccgttaggt caaattctgc tggaaaatca
    19981 ggtcatcact gaagaacaac tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct
    20041 gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc
    20101 agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt cctcgctgat
    20161 tgccgaaatg ccggcctccg tggcgctgca ttatgcggta ctgccgctgc gtctggaaaa
    20221 tgatgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac
    20281 gcgtaaagtc ggacgcaaag tgcgttacgt cattgttctg cggggacaaa ttgtcacggg
    20341 gttacgccac tggtatgcgc gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc
    20401 ggttcagcat cagtggctca cggaacagca ggccggtgaa atctggcggc aatatgtgcc
    20461 gcatcagttc ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat
    20521 taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac
    20581 cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca
    20641 agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact
    20701 ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc
    20761 tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc
    20821 tggggctgag cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg
    20881 cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg
    20941 tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg
    21001 atgaccgggc gcggctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac
    21061 ttgagcgcag tctggcggct attccagttg aagtgaaaag cgttacgaca gttgaagaac
    21121 tgcttgccca gcaaaaagcg tgcgatgctg cgccgaccct gcgttgtcgc agtgaagtcg
    21181 ggcagaatgc cctgcggctg gcacagttac ctgtcgccag agcgcaactg aacgatgcga
    21241 cgtttgctgc atcgccggaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct
    21301 acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat
    21361 taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctggacgatc
    21421 ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg
    21481 cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc attgaaaata
    21541 agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca
    21601 gcgccaaccc cattcaggca ttggcgaatt atacggtaca gtttgccgac aaccgccagt
    21661 atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg acagtacgac gcagcgcaaa
    21721 aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg
    21781 cgacccgtaa caaggctgaa gctttgcgtc tggcacgatt gctgtatcag caagaaccgg
    21841 caaatcttac ccgcctggat caactgacct ggcaactgat gcagaacgag cagtcacgcg
    21901 aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt gtcagccaga
    21961 ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca actccagcga
    22021 aggtggcgat tttatcaaaa cccttgccgc tggcggcgca acgtcagtgg caaagccagt
    22081 tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt
    22141 cctacgatgc cgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg
    22201 tggcgttgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg
    22261 cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg gcctggcaga
    22321 aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg
    22381 cggcaggaaa tggtgcggct cgcgatcgct ggctacaaca ggcagaaaaa cgtggactgg
    22441 gaagcaatgc gctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac
    22501 tcgcactgaa cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg
    22561 cgcgggcgac aatttatcgc caacgtcata atgtcccggc cgcggtgagt gatttgcgcg
    22621 ccgcgctgga actggaaccg aataatagca acacccaggc agcgcttggt tacgccttgt
    22681 gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggctac
    22741 cggacgatcc ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc
    22801 ctgcgacgca gcactacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa
    22861 ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtctg catgaggagg
    22921 tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc ggcgcaatga
    22981 gtaccgctaa caataatgtc ggcggcgcag caccagggaa aagctatcgt agctacggac
    23041 aactggaagc cgaataccgc atcggacgca atatgctgct ggaaggcgac ctgctttcag
    23101 tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc
    23161 cgatgtccgg caccggtcta cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg
    23221 tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca
    23281 gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct
    23341 ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt
    23401 ggacggcaga ttttcgcgtc agctggcatc agaaggtagc taacggacag actattgagc
    23461 cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg
    23521 gcggtgtcgg ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc
    23581 acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg
    23641 gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt
    23701 tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct
    23761 ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg ctgatgagtc
    23821 agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat
    23881 ttacccagcc agaacagcgc acgttattgt ttaagcgggc cgcagctgcg caacaggctg
    23941 gtctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt
    24001 cgtccgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca
    24061 gattatggag cgccgcgcct ggcgtaacgc cggatggctg gtacatcagc gcggaaattg
    24121 acgacctgaa ctggcgcagc gaagccgccc gtcagccttt gctaacatgg ttaaacaacg
    24181 agcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg
    24241 gaaacatgtc gacagatggc tatcgccaac tgctggaaca agttaaagca accggcgtta
    24301 atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt
    24361 atctacaggc cagcgccgat tgccaaagct ccgccccagc aagcggcatt gtttatgaac
    24421 tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac gcagaaattg
    24481 cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct
    24541 atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg
    24601 acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt
    24661 gctaaagaaa gaagatcgtt atcggcgaac gggccgataa ctcatgcagg aagcatagat
    24721 cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtgaacc
    24781 ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt
    24841 tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc
    24901 aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt
    24961 gtttttaaat aagaacttaa cctgtgtaaa gccctagtcg cggctggcta ccccggttat
    25021 cgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca
    25081 acccagcaaa ccctgcgaag cggagttgac gcgcgcattg ctttttaccg tattgtctaa
    25141 ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg
    25201 cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc tgcgaattgt
    25261 cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac
    25321 cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat
    25381 ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt
    25441 tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg
    25501 acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaatgaagaa
    25561 gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagt tcctagaacg
    25621 acattttaag tcaacaaatt accgcgccat ctctgcgctc acacgtccca ctacctcaaa
    25681 acatgtaaag ccttgcaagc cattgtgagg ccttatgtgt ctcagttttg tccctctttt
    25741 ttgtactaaa aaacatagca attgaggata aacctcatgc tattttcgct tatatgcctt
    25801 taaaggcatg gcgcttaaat agataaaaac accacaaaag cataaaaaac cacacagtaa
    25861 aaccgcaaca taaaacaata acagataatt aaaccaaaaa cagatagcgc attgtgataa
    25921 tcattcaata ctaaacaaaa tataaacagt ggagcaatat gtgattgact cattaagtta
    25981 gatataaaaa atacatattc aatcattaaa acgattgaat ggagaacttt tatgcgggcg
    26041 aaacttctgg gaatagttct gacaacccct attgcgatca gctcttttgc ttctaccgag
    26101 actatatcgt ttactcctga caacataaat gcggacatta gtcttggaac tctgagcgga
    26161 aaaacaaaag agcgcgttta tctagctgaa gaaggaggcc gaaaagtcag tcaactcgac
    26221 tggaaattca ataacgctgc aattattaaa ggtgcaatta attgggattt gatgccccag
    26281 atatctattg gggctgcggg ctggactact ctcggcagcc gaggtggcaa tatggtcgat
    26341 caggactgga tggattccag taaccccgga acctggacag atgaaagtag gcaccctgat
    26401 acacaactca attatgccaa cgaatttgat ctgaatatca aaggctggct cctcaacgaa
    26461 cccaattacc gcctgggact catggccgga tatcaggaaa gccgttatag ctttacagcc
    26521 agaggtggtt cctatatcta cagttctgag gagggattca gagatgatat cggctccttc
    26581 ccgaatggag aaagagcaat cggctacaaa caacgtttta aaatgcccta cattggcttg
    26641 actggaagtt atcgttatga agattttgag ctaggtggca catttaaata cagcggctgg
    26701 gtggaagcat ctgataacga tgagcactat gacccaggaa aaagaatcac ttatcgcagt
    26761 aaagtcaaag accaaaatta ctattctgtt gcagtcaatg caggttatta cgtaacacct
    26821 aacgcaaaag tttatgttga aggcgcatgg aatcgggtta cgaataaaaa aggtaatact
    26881 tcactttatg atcacaatga taacacttca gactacagca aaaatggtgc aggcatagaa
    26941 aactataact tcatcactac tgctggtctt aagtacacct tttaacgaag ttaaccagat
    27001 tttctccccg gtggtattgt ataaccccgg ggaatttgtt tacagttaca agtaggcaat
    27061 atccactaat tttgtaagca tttaacctat gtcataacgt gtaatctctt accgcaacac
    27121 aagacctcaa tatattcaaa tcttatcttt attccgatat tcagagacac aatgcgaaaa
    27181 ataattactc atttcaaagt tgttttaacg ttacttctac cagtaaccgt atctgcccag
    27241 cagatacagt ggcaatcctg tatggccagt caattcaacc actggtttgg tgaggaaaaa
    27301 ccgtctcctg acttactatg tggttatttg tctgttccat taaaatatac agacacaggc
    27361 ggagatgctt cttatgaaaa aaaatcacaa gtcaaactag cgttgacaaa attgccggca
    27421 aaaagcaagc ataaaggaag tatcctgata ataagtggtg gtcccgggtt accaggcata
    27481 aatccttata ttaactttga ctggccagtc acaaatcttc gtgagtcatg ggatattatt
    27541 ggatttgatc ctcgaggcgt cggacagtcc actccgacaa taaactgccg gcaatcagat
    27601 acagagactc aggaaaacat aaccgaaaag caacaagtat taaataaaat taatgcctgt
    27661 atccataata ccggagccga agtcattcgc catatcggct ctaacgaggc tgtatacgat
    27721 attgatcgta ttaggcaagc cttgggggat aaacaactga cagccgtggc gtattcgtat
    27781 ggaactcaaa ttgcagcctt atatgcagaa cgttttccct acaacgtaag atctatcgtt
    27841 cttgatggag tcgtcgatat cgatgacctg gaggacaact tcacatggca actcaaacag
    27901 gcacagagtt atcaggaaac gtttgatcgc tttgcatcct ggtgtgcgcg tacaaaaagt
    27961 tgcccgcttt cttcagacag agataaggca ataactcagt tccatgagct attatcaaaa
    28021 ttacatcaca aacctttatt agacagtaag agagaaaata tatcttcaga tgaactcata
    28081 tcattaacaa cagaccttct gctatggcgt tcatcatggc caacccttgc aactgccata
    28141 cgccagttct ctcaggggat tgtcagtaat gaaattgaaa ctgcgctcag tgctccgata
    28201 gcctcagaag agtcaagcga tgcttcgggg gtaatcctct gtgtagatca gggggatgag
    28261 caattaacac cagaagagcg aaaatcccga aaagacgctc ttgcgaatgc cttcccggct
    28321 attaactttg acaatggacg ttccgattca cctgattttt gtgaattatg gccaatacat
    28381 agcgacctga acaaaactcg cctgaaaaat actgttctgc cctctggttt actgtttata
    28441 gcacacaaat acgacccaac aacgccctgg attaatgccc gtaagatggc agagaaattt
    28501 tccagcccgt tactaacaat aaatggtgat gggcatacat tagctctcac cggagttaat
    28561 ttatgtgtag ataaagcagt tgtacatc
//
LOCUS       DAZUGK010000038        28329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZUGK010000038 DAZUGK010000000
VERSION     DAZUGK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.038"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..265
                     /locus_tag="ACHY5W_001474"
                     /pseudo
     CDS             1..265
                     /locus_tag="ACHY5W_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(451..1713)
                     /locus_tag="ACHY5W_001475"
     CDS             complement(451..1713)
                     /locus_tag="ACHY5W_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4446398.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPELLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(1911..1986)
                     /locus_tag="ACHY5W_001476"
     tRNA            complement(1911..1986)
                     /locus_tag="ACHY5W_001476"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Phe,seq:gaa)
     gene            complement(2092..2799)
                     /gene="yqgA"
                     /locus_tag="ACHY5W_001477"
     CDS             complement(2092..2799)
                     /gene="yqgA"
                     /locus_tag="ACHY5W_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4446399.1"
                     /translation="MVIGPFINASAVLLGGLLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2952..3035
                     /locus_tag="ACHY5W_001478"
     CDS             2952..3035
                     /locus_tag="ACHY5W_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446400.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            3198..5333
                     /gene="speC"
                     /locus_tag="ACHY5W_001479"
     CDS             3198..5333
                     /gene="speC"
                     /locus_tag="ACHY5W_001479"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFS4446401.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5383..6639)
                     /gene="nupG"
                     /locus_tag="ACHY5W_001480"
     CDS             complement(5383..6639)
                     /gene="nupG"
                     /locus_tag="ACHY5W_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFS4446402.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6841..7920)
                     /gene="mltC"
                     /locus_tag="ACHY5W_001481"
     CDS             complement(6841..7920)
                     /gene="mltC"
                     /locus_tag="ACHY5W_001481"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFS4446403.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7985..8260)
                     /gene="yggX"
                     /locus_tag="ACHY5W_001482"
     CDS             complement(7985..8260)
                     /gene="yggX"
                     /locus_tag="ACHY5W_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFS4446404.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8288..9340)
                     /gene="mutY"
                     /locus_tag="ACHY5W_001483"
     CDS             complement(8288..9340)
                     /gene="mutY"
                     /locus_tag="ACHY5W_001483"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4446405.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWSGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9501..10220
                     /gene="trmB"
                     /locus_tag="ACHY5W_001484"
     CDS             9501..10220
                     /gene="trmB"
                     /locus_tag="ACHY5W_001484"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4446406.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFFGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10220..10546
                     /gene="yggL"
                     /locus_tag="ACHY5W_001485"
     CDS             10220..10546
                     /gene="yggL"
                     /locus_tag="ACHY5W_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFS4446407.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10730..11449
                     /gene="yggN"
                     /locus_tag="ACHY5W_001486"
     CDS             10730..11449
                     /gene="yggN"
                     /locus_tag="ACHY5W_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFS4446408.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11625..12671
                     /gene="ansB"
                     /locus_tag="ACHY5W_001487"
     CDS             11625..12671
                     /gene="ansB"
                     /locus_tag="ACHY5W_001487"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFS4446409.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQNMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12788..13795
                     /gene="yggM"
                     /locus_tag="ACHY5W_001488"
     CDS             12788..13795
                     /gene="yggM"
                     /locus_tag="ACHY5W_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFS4446410.1"
                     /translation="MKKQWIVGTALLVLMTGNVWADGEPPTENILKAQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13864..15000)
                     /gene="hemW"
                     /locus_tag="ACHY5W_001489"
     CDS             complement(13864..15000)
                     /gene="hemW"
                     /locus_tag="ACHY5W_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4446411.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEANRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRMEFSQYTGLS
                     EEVIRPQLEEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14993..15586)
                     /gene="rdgB"
                     /locus_tag="ACHY5W_001490"
     CDS             complement(14993..15586)
                     /gene="rdgB"
                     /locus_tag="ACHY5W_001490"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFS4446412.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15594..15884)
                     /gene="yggU"
                     /locus_tag="ACHY5W_001491"
     CDS             complement(15594..15884)
                     /gene="yggU"
                     /locus_tag="ACHY5W_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFS4446413.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSLVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15881..16447)
                     /gene="yggT"
                     /locus_tag="ACHY5W_001492"
     CDS             complement(15881..16447)
                     /gene="yggT"
                     /locus_tag="ACHY5W_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFS4446414.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16465..17169)
                     /gene="yggS"
                     /locus_tag="ACHY5W_001493"
     CDS             complement(16465..17169)
                     /gene="yggS"
                     /locus_tag="ACHY5W_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFS4446415.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17187..18167
                     /gene="yggR"
                     /locus_tag="ACHY5W_001494"
     CDS             17187..18167
                     /gene="yggR"
                     /locus_tag="ACHY5W_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFS4446416.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLKNGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSCCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLNCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVKRLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18358..18774)
                     /gene="ruvX"
                     /locus_tag="ACHY5W_001495"
     CDS             complement(18358..18774)
                     /gene="ruvX"
                     /locus_tag="ACHY5W_001495"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4446417.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18774..19337)
                     /gene="yqgE"
                     /locus_tag="ACHY5W_001496"
     CDS             complement(18774..19337)
                     /gene="yqgE"
                     /locus_tag="ACHY5W_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFS4446418.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19446..20396)
                     /gene="gshB"
                     /locus_tag="ACHY5W_001497"
     CDS             complement(19446..20396)
                     /gene="gshB"
                     /locus_tag="ACHY5W_001497"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFS4446419.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LSNGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(20409..21140)
                     /gene="rsmE"
                     /locus_tag="ACHY5W_001498"
     CDS             complement(20409..21140)
                     /gene="rsmE"
                     /locus_tag="ACHY5W_001498"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4446420.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21220..21927)
                     /gene="endA"
                     /locus_tag="ACHY5W_001499"
     CDS             complement(21220..21927)
                     /gene="endA"
                     /locus_tag="ACHY5W_001499"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFS4446421.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCVMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(22022..22519)
                     /gene="yggI"
                     /locus_tag="ACHY5W_001500"
     CDS             complement(22022..22519)
                     /gene="yggI"
                     /locus_tag="ACHY5W_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFS4446422.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22596..23990)
                     /gene="galP"
                     /locus_tag="ACHY5W_001501"
     CDS             complement(22596..23990)
                     /gene="galP"
                     /locus_tag="ACHY5W_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFS4446423.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24427..25581)
                     /gene="metK"
                     /locus_tag="ACHY5W_001502"
     CDS             complement(24427..25581)
                     /gene="metK"
                     /locus_tag="ACHY5W_001502"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4446424.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25637..25888
                     /gene="yqgD"
                     /locus_tag="ACHY5W_001503"
     CDS             25637..25888
                     /gene="yqgD"
                     /locus_tag="ACHY5W_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFS4446425.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25999..26142
                     /gene="yqgG"
                     /locus_tag="ACHY5W_001504"
     CDS             25999..26142
                     /gene="yqgG"
                     /locus_tag="ACHY5W_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFS4446426.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26236..26367
                     /gene="yqgB"
                     /locus_tag="ACHY5W_001505"
     CDS             26236..26367
                     /gene="yqgB"
                     /locus_tag="ACHY5W_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFS4446427.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26376..>28329
                     /gene="speA"
                     /locus_tag="ACHY5W_001506"
     CDS             26376..>28329
                     /gene="speA"
                     /locus_tag="ACHY5W_001506"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFS4446428.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENSLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYG"
BASE COUNT         6942 a         7065 c         7454 g         6868 t
ORIGIN      
        1 cactctggcc acactggagc gtattctgag cctgctgtcg gcctttgagg tcgtggtata
       61 gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc acgttatcag
      121 caagcgttac actcagcgca ttgagcgaca taatctgaga caacatctgg caaggctggg
      181 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
      241 tctgaacata aaacactatc aataagttgg agtcattacc tggtttttga tgccagccat
      301 aacgtgtttg actacaaagt attacgaaag aactttaatg agcccctgga ataattcatt
      361 aaagtaattt acttcgctat cctttttcca gttactcaca aagaaaaata ttcagaaaat
      421 aattcccggg ctggctgccc gggatgatta ctattttaat ggattgttaa tctttgcata
      481 ttcaaacggg ctgatacatt gttctctgtt cgcatccaga aaatctgccc accactgtag
      541 cataagtcga cgttcttcca gatgttcggc tttatggata tacgcagcac gtacaccatt
      601 acgctcctga tggctcatct ggcgttctac agcatcgcgg gaccacaaac ctgattctat
      661 taatgcacta catgccatcg cccggaatcc atgaccacag acatcctggg ttgtgtcata
      721 ccccataacc ctcagggctt tatttacggt gttttcactg attggtttac gtgggtcatg
      781 tgctcccgta aagatcagac cattttcacc tgcccatgtc tgaagttctg ccagtatggc
      841 tacagcctgt tggctgagtg gcacaagatg tggtgttcgc atcttggagc cccggtatga
      901 aaattttacg cctggcagag gttcgcgttc ggctggaata gtccacatgg cattgtcaat
      961 atcaatttca gaccagcggg catatctgag ttcactggaa cgaataaaaa ccagtaaatt
     1021 cagcataact gccagtcggg tgacaggctg gcctttataa ccgtcaatac gcgacagaag
     1081 ttcaggcagg cgtgaaagat ccagagcagg gcgatgctgg cgttttaccg tggtcaatgc
     1141 accagccata tccagagccg ggttatatct gatgatgcca gactgtacgg cataacgcat
     1201 tacagcagat attctttgct gcagacgact ggcgatttca taaatttgtt tagcttctgc
     1261 agcacgaaca gggataagca gctccggggt actgagtgtt gtgatatccc ggttgccgat
     1321 aaaggggaaa acgtgtgtct cgaggctttt gagtacttta tcgctgtgtg attgtgccca
     1381 ttgtttatta ctggcatgcc agcgtcgtgc aatatgttca aaagagagtt gtaaatcagg
     1441 gactactttt ttagctcttt tctgttcgca agggtcaacc ccctccgaaa tcagttttcg
     1501 ggcctcatcc cgttttgtac gagcttcgga gagagaaact tcgggataca caccaagtgc
     1561 cagggttttc tccttaccca gaatacgata acggagacgc cagtaacggg aaccattagg
     1621 atgaaccagc aggaacatgc cggccccgtc agtgagttta taagccttgt cagtgggctt
     1681 tgcagcccgg atttttgcgt cagtcagtgc catagttcag ccttctgaat gcgttaaaga
     1741 gggtacaaat tctgtcgaac tggggtatac ccgcaattat acccacacaa tggggttgat
     1801 gtggggggaa tctggttgag ttcggtagaa ttgatttgga ggcagaacgc ttaaattgtg
     1861 gcgttctgaa acgaaaaacg gacctccgtg gaggtccgtt gatatgaatt tggtgcccgg
     1921 actcggaatc gaaccaagga cacggggatt ttcaatcccc tgctctaccg actgagctat
     1981 ccgggcaacg gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa tttcttcaat
     2041 ttactgcaga ctgcacaatc tatcatcact ttgccgctat tgcacgcatt gtcaggcaaa
     2101 ccaggcggtc caggcggcgg aaagcggcat tgccagaagg agtgctggaa gcatgttgac
     2161 caccgggaac attttaatgc cacagatgcg taatccggtt gccagcagca ataaaccgcc
     2221 tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc ggtaatatca gcgcggcagc
     2281 ccacgccagc gttaactgga tgatcagtaa tgggatacta atcaccgata ccgcaatacc
     2341 cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg cgattaaaat
     2401 actcggatcg ccggtcatcc cttcgttcat cgccccgaag atcccggtgc cgctggcgca
     2461 aaacaggaca ataatcgcga cataattctg aataaaagat tcatgcgctg gcttcttacg
     2521 tgagtgacga aacagatttt gtgctttggc gaccgctgta ttgacgcctt tctccagcag
     2581 acaaatttcg ccgattaatg ctccgagtaa ggttgctaaa accatcgctg gaaggttggc
     2641 gcatttcacc accagtaaaa taccaatccc cagcgatgcc agaccaaaaa ttgatgtcat
     2701 ggagacgcgg atacgttccg gtaagcgttg gctaagcagt gcaccgagca agccacccag
     2761 taatactgca cttgcgttga taaaagggcc gatgaccaca agagttcctg ttaactgtat
     2821 tgtttgattt tcttattatg aactttttgc cttgttgatg gcttgtctgg tcgcaaagtc
     2881 ctggcttgca cgtcttagcg aaaggtgcca tgattgcgcg aattttctcc tctctgtacg
     2941 gagtttgccc gatgcacgcc atctccttac attctctcgc ttatcgccgt ttcgcgcgaa
     3001 acccttctct ttttatgcta ctgctcatgc ggtgaagtta acgcacgttc actgcaggac
     3061 aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg gcggtcggag ctggtgacca
     3121 gtttgaccca tatctcatgg ggcagggttt tacaccttgt ccggtattct tacttccccg
     3181 aaacgggttt tgcgcttatg aaatcaatga atattgccgc cagtagtgaa ctggtatccc
     3241 gactttctac tcatcgccgc gtggtggcgt tgggagatac tgattttacg gacgtcgcgg
     3301 cagtcgtcat taccgctgcg gatagtcgca gtggcattct tacgttgctt aagcgcaccg
     3361 gttttcatct accggtgttt ttgtattccg aacatgctgt tgaattacct gcgggcgtta
     3421 cggcggtaat caacggcaac gagcagcatt ggctggagct ggaatccgca gcctgtcagt
     3481 atgaagagaa tttgctgcca ccgttttatg acacgctgac gcagtacgtt gagatgggca
     3541 acagcacctt tgcttgccct gggcatcaac atggtgcgtt ttttaaaaag catcctgccg
     3601 gacgccattt ttacgatttc tttggtgaga acgtctttcg cgccgatatg tgtaatgctg
     3661 acgtaaaatt gggcgatctg cttattcatg aaggatcggc gaaagatgcg cagaaattcg
     3721 cagccaaagt ctttcatgcc gataaaacct attttgtact gaacggcaca tcggcagcga
     3781 ataaagtggt gacgaatgcg ctattaacgc gtggcgatct ggtgctcttc gaccgtaaca
     3841 accataagtc gaatcatcac ggcgcgctga ttcaggcggg ggcgacgccg gtctatctgg
     3901 aagcttcacg caacccgttt ggtttcattg gcggtattga tgcgcactgt tttaatgaag
     3961 agtatctgcg ccagcaaatt cgcgacgttg cgccagaaaa agccgacctg ccgcgcccgt
     4021 ttcgcctggc gattattcag ctgggaacct atgacggcac tgtctataac gcccgtcagg
     4081 tgatcgatac cattgggcat ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt
     4141 acgaacagtt tatcccgatg atggcggata gctcgccgct gctgttagaa cttaacgaaa
     4201 acgatccagg gatctttgtg actcagtcgg tgcacaaaca gcaggcggga ttctcacaga
     4261 cgtcgcagat ccataaaaaa gataaccata tccgcggaca ggcgcgtttt tgcccgcata
     4321 agcggttgaa taacgccttt atgctccatg cttctaccag ccctttctat ccgctgtttg
     4381 ctgcactgga tgttaacgcc aaaattcatg aaggggagag tgggcgtcgg ctgtgggctg
     4441 agtgcgttga gttgggtatt gagtcgcgca aggctattct tgcgcgctgt aagctgttcc
     4501 gcccgtttat cccgcccgtt gttgatggca aattgtggca ggattatccg acatcagtgt
     4561 tagccagcga ccgccgtttt ttcagttttg agccgggggc gaagtggcac ggctttgaag
     4621 gatatgccgc ggatcagtat tttgttgatc cgtgcaagct gttactcact acaccaggta
     4681 tcgatgccga aaccggcgag tatagcgact ttggcgttcc ggcgacgatt ctggcgcact
     4741 atctgcgtga gaacggcatt gtgccggaga agtgcgatct caactccatt ctgttcttat
     4801 taactccggc ggaaagccaa gagaagctgg cacaactggt ggcgatgctg gcgcaatttg
     4861 aacagcatat tgaggatgac tcgccgctgg ctgaggtgtt gccgagcgtt tataacaaat
     4921 atccggtgcg ctatcgcgac tacaccctgc gccagttgtg tcaggagatg cacgatctgt
     4981 atgtcagttt cgacgtcaaa gacctacaaa aagcgatgtt ccgccagcag agtttcccgt
     5041 cagtggtgat gaacccccag gatgcgcata gcgcttatat tcgcggtgac gtggagttgg
     5101 tgcggattcg tgatgccgaa gggcgaattg cggcagaagg ggcgttgcct tatccacctg
     5161 gcgtgctttg cgtggtaccc ggggaagtct ggggtggggc ggttcaacgt tatttccttg
     5221 cactggaaga aggggtgaat ttgttgccgg gattttcgcc ggagctgcaa ggtgtttata
     5281 gcgaaaccga tgcggatggc atgaaacggt tgtacggtta tgtgttgaag taagaataaa
     5341 aaaaacgggt cgccttctgg cgacccgttt ttctttgcgt aattagtggc taaccgtctg
     5401 tgtacctgtc gggacacgaa cgtgtttata tttgaacatc gccatgaacg cgaaggccag
     5461 aaccacggag taaccagcga aaatcaacca tacggtctgc cagtcggtaa tgccgttttg
     5521 ggtgtacatc tcaacaactt taccgctcac gatgccgccg aggatacagc cgaagccgtt
     5581 agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt gccgggctaa cttctttttc
     5641 gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga
     5701 cagtaccagc agtacagtac cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg
     5761 caggatccac gccacaatac tgatcatcat tacgttctta ataccgtagc ggcttaagaa
     5821 gaacgggatg gtcagaatga acagggtttc agagatctgc gaaatcgaca tgatgattga
     5881 cgcatgctgc acaataaagc tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa
     5941 ggtattaccg aacatgttgg taatctgcag ttccgcgccc agcagcattg agaagatgaa
     6001 gaagattgcc atacgcttgt ttttaaacag cgcgaatgca tcgaggccca gcagggttgt
     6061 ccagctctga ttcgcttgct gtttagcaac cggaatatgc ggcagagtca gggtaaacag
     6121 aaccagaatg gcggaaagtg ctgcgccaat atacagctgc atgtggctta attcgaagcc
     6181 agacaggctc accacccaca ttgccatgat aaagccgatg gtgccccaga tacggattgg
     6241 cgggaagtca gtaacgatat ccatcccggc attttgcagg cgatagtaag agatggtgtt
     6301 gattaaccca agcgttggca tataagcaaa cgagttaatc aatatcacaa ggaacatcgc
     6361 ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat
     6421 ggcatatacc catttcgcac ttaaccattt gtcggccaca atccccagca gcgcaggcat
     6481 aaagaccgcg gcgataccca gtgaactata aactgcgcca atagaagcac cgtcaaactt
     6541 cagggtaaca aacatatagg agccgagggt cgtcagccaa cttccccaca gacagaactg
     6601 cagaaaagag aggattttca gctgcagctt aagattcatg ttaatttcct cacatcgtga
     6661 tgcggatgag tgtttgaaaa agcacatttg accgtgcgag atgatgggat tctatcaact
     6721 tcgcggactg gttttttgtt acctgtggca aataattgca aacattccca atttgcaaaa
     6781 cgaaattgtg atgtggataa catttttgcc cctgagcatc gtcaggggcg gttaatggaa
     6841 ttatcggcgg cggtaggatt tttgcgcggt attcacttta taaagataac ggcgagattc
     6901 cgcagaggga tggcgggtcg tcagcgtttg ataaacatcg cccggcgtca tggtgttaat
     6961 aatattagcc gcctgaatct tatcattcga aaagactcgc agcacgctgc ctgcgccgcc
     7021 gttataggcg gtgatgacgg cataacgccg ggatgtaggg ttatcaattc cgccgagata
     7081 aacattgttc agcatcgcca gatacgcggt gccggtatca atattgctgg caggatcaaa
     7141 caagaaactg cggctcggcg tgccggattt cccctgcgag cggaacacat ctttcccggc
     7201 agtatgttgt accacctgca ttaatcccag cgcatcggaa cggctgaccg catacgggtt
     7261 aaaggaagat tcagtctgca taattgccag aatcagcgac tcatcaacgc catatttccg
     7321 tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt ttatcaaggt ggtttggcac
     7381 catgttaatg gtgacgctgt agatgatacg tagcccgttg ctgcggcttt tcaggcggtt
     7441 tttcagcaga taatccgcga agttgcttgc gcgaccttcc cagcgaatcg gctgcccggt
     7501 gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc
     7561 ggaatagaga tcgaccgaac tcggatcgtc acccatcaat aacgttttga taattgcccg
     7621 gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg atagtaatcg taccgtcatc
     7681 gaagttgatg tggctgcggg tctgatattg atcggtgtat ttcacgtagt ccttaggacc
     7741 agcgatcacc acctctttga agccccagat gttctcaata ttgtgggcaa attgccccat
     7801 cagaatatca aaaccgttgg tatctttgac ccaggcttcg ttataggtat cgcctttttt
     7861 ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc agcgcgagat attttttcat
     7921 cattccgggt gcgtgttgtg tttgttttta agcaagctgc cggaggcgtg ctccggcact
     7981 gtttttattt tttatcttcc ggcgtatagc cctcgatatg cacctcttta ccctcgaaca
     8041 ggaagttgac catctcctgc tccagcagct tgcggtgctc ggcattcatc atgttgagtt
     8101 tcttttcatt aatcagcatg gtttgtttgt gctgccactg cgcccaggct tctttggaga
     8161 tctcgttata gatgcgtttt cccagctcgc cggggtacag ctgaaaatcc tgaccttctg
     8221 cttcacgttg caggaaagta caaaaaatcg ttctgctcat aaatcatcct ctttatcgac
     8281 tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa caaacgctcc acgggagccg
     8341 ccaggccaac tgacggcggt tgcgctaagt tataccagag cgcattgcct tcatccatgc
     8401 agccggtgaa tgacgacacg ggaagccaca taggcacaat atctaagtgg aaatggctga
     8461 aggtatgccg aaacgcggtc agctgcgtca ggttatcggc agcaatctgc cgttgcgcca
     8521 gccactgccg caaactttct tcgtcggcaa actgcgggaa acagtataaa ccgccccaca
     8581 atccgctcgg cggacgctgc gccagcaata cttcatcttc gtgctgtaac aggagaaagt
     8641 agccggtgcg ctccggcagc gtctgtttcg gttttttgcc cggataaagc gaccagctat
     8701 tgttggtggc ggcaatacat ccgttttgta gcggacagag cgaacatttc ggcttcgaac
     8761 gcgtgcaaat catcgcgccc aaatccatca tcgcctgatt aaaccgttcc acgccgaccg
     8821 cgggcgtcac ctgctcgctc aaactccata atttattctc gacctctttt ttcccagacc
     8881 agccgcttac agcatagcag cgcgccagca cccgtttgac gttaccgtcg agaatcggaa
     8941 agtgcttacc cagagaaagc gagagaatcg cgcctgcggt ggaacgcccg acgcccggca
     9001 gtgctgcaac ttcctcaaag gtttccggga atttaccgcc gtgtaaggtc gccacttgtt
     9061 gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga
     9121 gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt cgggaagcgc gccataaagc
     9181 gttcaaaata ggggataacg gtcgcaacct gagtttgttg caacatcact tctgatagcc
     9241 atactttgta gggcgtcttg tcaatttgcc agggcagagt ttttcgcccg tatttatcgt
     9301 accagtccag aacctgggct gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg
     9361 ttgggggcca gattgcagca cagcgacggc agggtgtaaa ccggaacttt ccgcagccac
     9421 ggccttatca tgcttgcatc cggcaactaa ctttggataa tgcccgtttt cagaacactt
     9481 tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc accggaattt gatgaaaacg
     9541 gccgcccact gcgccgtatc cgtagttttg tgcgccgcca ggggcgactg accaaaggcc
     9601 aggaacatgc gctggaaaac tactggccgg tgatgggcgt tgagttcagc gaagatatgc
     9661 tggatttccc cgcgcttttt ggccgtgaag cgccggtgac gcttgagatt ggttttggca
     9721 tgggggcgtc gctggtggca atggctaaag atcgccccga gcaggacttc ttcggcattg
     9781 aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca
     9841 acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca taaaatgatt cctgacaatt
     9901 cattgcgcat ggtgcagctc tttttccctg acccgtggca caaagcgcgc cataataaac
     9961 gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag caaactgcag ctggggggcg
    10021 tattccatat ggcgaccgac tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt
    10081 ctattgacgg ttataaaaac ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac
    10141 gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg tcacggagta tgggacttaa
    10201 tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca
    10261 catcgacgaa ttccaggaat taggattttc ggtggcatgg cgattcccgg aaggtacatc
    10321 ggaagaacag attgataaaa ccgttgatga ttttattaat gaggttatcg aaccgaacaa
    10381 actggccttt gacggcagcg gttatctggc ctgggaaggt ctgatctgca tgcaggaaat
    10441 cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag tggctggaag agcgcaaact
    10501 ggatgaggta cgcaccagcg aacttttcga cgtttggtgg gactaagaaa gcatacgggc
    10561 gatgacaaat gcaaaactgc ctgatgcgct acgcttatca tgcctacaaa gatgcacgat
    10621 cgagtaggcg ggataaggcg tttacgccgc atccggcatg gaaaacgcgt actttgttat
    10681 caatctgggg ccagcaaatg ctggcctgat ttgttcttga gggaagacta tgatgcgcaa
    10741 aatgctgctg gcggcagcac tttcagtgac ggcaatgacc gctcacgccg actaccagtg
    10801 cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa accgtgcagg tgaagggcga
    10861 aaacggcaat ctggtgatca cgccagacgg caacgtgatg tataacggta agcaatattc
    10921 cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag gctgaactac gtagcacgct
    10981 gccgtggatt gatgaaggcg cgaaaagccg cgtcgagaaa gcccgtattg ctctggataa
    11041 aattatcgtt caggagatgg gcgaaagcag caaaatgcgc agccgtctga ccaaacttga
    11101 tgcgcagctg aaagagcaga tgaaccgtat tatcgaaacg cgcagcgatg gcctgacgtt
    11161 ccactataaa gccattgatc aggttcgcgc tgaaggccag caattagtga atcaggcaat
    11221 gggcggaatt ttacaggaca gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg
    11281 cggcaaccca ttacagaatg tgctgggaag tctgggcggt ctgcaatcct caatccaaac
    11341 cgagtggaaa aagcaggaaa aagatttcca gcagtttggc aaagatgttt gtagccgcgt
    11401 tgtgactctg gaagatagcc gcaaagccct ggtcgggaat ttaaaataat cctctatttt
    11461 aagacggtat aatacttttt tatgccgttt aattcttcgt tttgttacct gcctctaact
    11521 ttgtagatct ccaaaatata ttcacgttgt aaattgttta acgtcaaatt tcccatgcaa
    11581 agctaaggga taatgcgtag cgttcacgta actggaggaa tgaaatggag tttttcaaaa
    11641 agacggcact tgccgcactg gttatgggtt ttagtggtgc agcattggca ttacccaata
    11701 tcaccatttt agcaaccggc gggaccattg ccggtggtgg tgactccgca accaaatcta
    11761 actacacagc gggtaaagtt ggcgtagaaa atctggttaa tgcggtgccg caactgaagg
    11821 acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg ctcccagaac atgaacgatg
    11881 atgtctggct gacactggcg aaaaaaatta acaccgactg cgataaaacc gacggtttcg
    11941 tcattactca cggtaccgac acgatggaag aaaccgctta cttcctcgac ctgacggtga
    12001 aatgcgacaa accggtggtg atggtcggcg caatgcgccc gtccacgtcc atgagcgcag
    12061 acggtccatt caacctgtat aacgcggtag tgactgcagc tgataaagcc tccgctaatc
    12121 gtggcgtgct ggtggtgatg aacgacaccg tactggatgg gcgggatgtc actaaaacca
    12181 acaccaccga cgtagcgacc ttcaagtctg ttaactacgg tcctctgggt tacattcaca
    12241 acggtaagat tgactaccaa cgtaccccgg cacgtaagca caccagcgac acgccgttcg
    12301 atgtctctaa gctgaatgaa ctgccgaaag tcggcattgt ttataactac gctaacgcat
    12361 ccgatcttcc ggctaaagca ctggtagatg cgggctatga tggcatcgtt agcgctggtg
    12421 tgggtaacgg caacctgtat aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg
    12481 gcactgcagt cgtgcgttct tcccgcgtac cgacgggcgc taccactcag gatgccgaag
    12541 tggatgatgc gaaatacggc ttcgtcgcct ctggcacgtt gaacccgcaa aaagcgcgcg
    12601 ttctgctgca actggctctg acgcaaacca aagatccgca gcagatccag cagatcttca
    12661 atcagtacta accacctcgc cccggtatcg tgccggggct tttacacttc agacttacgt
    12721 ccattgccaa tttttattac cctgatgata atcaccggaa taaattattc cgcgcgaggg
    12781 ttttcgggtg aaaaagcaat ggattgttgg cacggcgctg cttgtgttga tgactggtaa
    12841 tgtctgggca gatggcgaac cgccaactga aaatatctta aaagctcaat tcaaaaagca
    12901 gtatcacggc attctcaagc ttgatgccat caccttaaaa aatcttgatg ctaagggtaa
    12961 tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt gacgatctct atacctgggt
    13021 cggtcagttg gcagattacg aactgctcga acagacctgg acgaaagata aaccggtaaa
    13081 attctcggcg atgttaacca gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta
    13141 ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc gacgatatca aaacgaataa
    13201 taaatatctg atcgtgaata gcgaagattt caattatcgc tttagtcagc ttgagtctgc
    13261 gttgaatacc cagaagaact cgattcctgc gctggaaaag gaagtgaaag cgcttgataa
    13321 gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg ggaaaagatg caaacggtaa
    13381 gcaaatgacc cgcgaagacg cctttaaaaa aattcaccaa cagcgtgatg agtttaataa
    13441 acagaatgat agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc aaccggcgat
    13501 tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg ccgattcagc agaagcgaga
    13561 tttcgatatc aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa
    13621 attgcaggat gactggataa cgctcgaaaa agggcaatat cctttaacga tgaaagtttc
    13681 agaaataaac agtaaaaaag tcgcgatttt gatgaaaatt gacgatatca accaggctaa
    13741 tgaacgctgg aaaaaagata ccgaacaact gcggcgcaat ggggtgatta agtaattttt
    13801 gcgataacgc atgagttgtg gccggatgct gcgtacacgc acgccgcatc cggcaataca
    13861 aatttactcc gccagaaaaa gctccagcag cgaatttaaa aacagcttcc catgttccgt
    13921 tatttgccag taatccgcac attctgtgag ataaccctga gcgatagcct cttctaactg
    13981 tgggcgaata acttcttcag aaaggccagt atactggcta aactccatgc gcggcgcggc
    14041 ctccagcaga cggaagcgat tcataaagaa ctcaaacggc ttatctgcgg cttcgacatc
    14101 acgctggctt tccagatacc ttccttgcat aaaaccacgc ggatgacgcg ttttggtggt
    14161 acgcagaatg cgaccatccg ggaaggtgac tttgccgtgc gcgccgcagc caataccgat
    14221 gtagtcacca aatcgccagt agttgagatt gtgctggcac tgataaccgg gtttggcgta
    14281 agcggaagtt tcatattgct gataacccgc tgcggttaat aactgatgcc cctgttcgaa
    14341 aatatcccac agcgcgtcat cgtccggcag caccggtggt cgcgaaccaa acagcgtatt
    14401 gggttcgatg gtcagttgat accaggaaag atgcggcgga ttcagttcaa tggcctggcg
    14461 tagatcgcca agcgcctctt ccagtgattg atccggcagc ccatgcatca aatcaaggtt
    14521 aaagctacgc agccccagcc cgctcgccag ctttgccgct cgattcgctt cttgcgggcc
    14581 atgaatacgc ccaagtcgtt tcagcttttc ttcgctaaaa ctctgcacac caatagagat
    14641 gcggttcaca ccagcacgct gataatcgac aaagcgatcc gcttctaccg tgcctgggtt
    14701 cgcttccata gtaatttctg catccgctgc cagcggcaaa cgcgcacgca cgccgtccag
    14761 cagcgtttgc atcgccgggc cggaaagcag gctcggcgta ccaccgccaa taaagattgt
    14821 ctttacttca cggccctgag cgtaagccac atcattgtcc agatcgcaaa gcagatgctg
    14881 aacataatcg tcgtgcggca cttctccttt caacgcgtga gagttgaaat cgcagtacgg
    14941 gcatttctgc acgcaccacg ggatgtgaat gtagagactc agcggcggta atttaaccat
    15001 tacgtaaagc gtccagcagc agtttcaatg cctgaccacg gtgggaaatg gcgctctttt
    15061 cttcgcgggt cagttcggca gcggttttcc cttcggaagg tacgaagaag attggatcat
    15121 aaccaaagcc gccagtaccc gctggttcac gagtaatcat gcccggccaa ctaccgtggc
    15181 acaccagcgg agtcggatct tccgcgtgac gcagatatac cagcacgcag tggaaacgcg
    15241 cctgacgttg gtcgtccggt acgtctttca gtgtttccag cagtttttgc agattctttt
    15301 gatcggtcgc gtcttcaccg gaataacgcg cggagtaaat ccccggcgca ccgccaagta
    15361 catctaccgc cagaccggag tcgtcggcaa ttgccggtaa accggtcact tttgccgcat
    15421 ggcgcgcttt cagaatcgcg ttttcgataa aggtcaggcc ggtttcttca gcggaatcta
    15481 cgccgaggtc tgtttgggcc acgatatcaa gaccgaagtc gctaagcagc gacgccagct
    15541 cacgcacttt accggcattg ccggttgcga ggacaacttt ttgcatagga tacctaatta
    15601 attaacgccg cgatttctgg cgggatttgt tgcggattaa tgattttaat ttgtttgtgg
    15661 cggccaagtt cgcctttttc aatcaccact aggcttttgg caacccggaa ttgcttaccg
    15721 agaaacttca ccagatggct gttggcctga ccgtcaaccg gtggcgcggt aatggcgact
    15781 tttacctcgt cgccatgtaa accgacaata gaatcacggc tggctttcgg ctgaatatag
    15841 agccgtaaaa ccagaccgtc atcattaact gttacggcac tcataacgcc atccacagcc
    15901 ccggcagcag catgtttccg gttgcctgta atacttctgc gacacccata ttgatgacat
    15961 acagcagcag aacgaggatc atcggcgaga aatcaatccc acccattgct ggtagcaggc
    16021 ggcgaatcgg gcgcagcagc ggatcggcca gctgaatcag cacgtattca atcgggctac
    16081 gaccctggct tacccagctc ataatcgcca tcaccagcag gacccagaaa atcagcaggc
    16141 cgatggtttt cagcagaatc agtaaaccgg caatccagat gattggcagg aaggtcacca
    16201 ctttaaacag cacaatggct ttgataaaac tgagaatata ggcaaccagc agcgaggcgc
    16261 tgtcaatggg tcccattgcc ggaataacgc ggcgcagtgg cccgataatt ggctgtgtta
    16321 ccttcactac aaactgtgag aaggggttgt aaaaatcaca atgagcccac tgcatccaga
    16381 tgcgtaataa cagcaccatg gtatacagct caatgaccgt tgaaagcagg aaagtcaacg
    16441 tattcatggc gttcctttaa ttccttattt tttagagtaa tcacgcgcac caaaaattgc
    16501 agtgccgata cgaaccatcg tgctacctgc cgcaatggcg gcttccatat cgtccgacat
    16561 tcccagagag agcgtgtcga tatgcgggta gcgcgttttc agtccggcaa atgctacagc
    16621 catttggcgt gcaacttcaa actgccttac atattctgac tcaggcgcag ggattgccat
    16681 cagcccgcgc agacgtaaac gcggtagttc agcgaccgca gctgccagct cgtccagttc
    16741 tgccagttga atcccggact tactgttttc atcactaata ttaatttgaa tcagcacgtt
    16801 aagaggggga agttctgccg ggcgctgatc gttgaggcgg gtagcgatgc gcaaacggtc
    16861 gatggtatga caccagtcga aatgctctgc caccaggcgg cttttattag actgcaacgg
    16921 gccaataaaa tgccattcta atcctgttac gcccagttcc tgaaagtggc gaattttatc
    16981 taccccttcc tgaacgtagt tttcaccaaa ttgacgctgc ccggcatcaa tggcttctgc
    17041 gatggcgctc gcaggttttg ttttactgac tgcaagcagc gtaatttctt ctggagaacg
    17101 gccgcaacgc gttgcagccg ctgagatttt gtcccggacc tgtgccaggt tatgcgcaat
    17161 atcgttcatt ttccgaggat cttagtatga atatggaaga aattgtggcc cttagtgtaa
    17221 agcataacgt ctcggatcta cacctgtgca gcgcctggcc cgcacgatgg cgcattcgcg
    17281 ggctaatgga agctgcaccg tttgatgcgc cggacgtcga agagctactg cgggagtggc
    17341 tggatgacga tcagcgggca atattgctga agaatggtca gctggatttt gccgtgtcgc
    17401 tggcggaaaa ccagcgattg cgcggcagtg cgttcgcaca acggcaaggt atttctctgg
    17461 cgttacggtt gttaccttcg tgctgcccgc agctcgaaca gcttggcgca ccaccggtat
    17521 tgccggaatt actcaagagc gagaatggcc tgattctggt gacgggggcg acggggagtg
    17581 gtaaatccac cacgctggcg gcaatggttg gctatctcaa tcaacatgcc gatgcgcata
    17641 ttctgacgct ggaagatcct gttgaatatc tctatgccag ccagcgatgt ttgatccagc
    17701 agcgggaaat cggtttgaat tgtatgacgt tcgcatcggg attgcgggcc gcattgcggg
    17761 aagatcctga tgtgattttg ctcggagagc tgcgtgacag cgagacaatc cgtctggcgc
    17821 tgacggcggc agaaaccggg catttggtgc tggcaacatt acatacgcgt ggtgcggcgc
    17881 aggcagttaa gcggctggtg gattcatttc cggcgcagga aaaagatccc gtacgtaatc
    17941 aactggcagg tagtttacgg gcagtgttgt cacaaaagct ggaagtggat aaacaggaag
    18001 gacgcgtggc gctgtttgaa ttgctgatta acacacccgc ggtggggaat ttgattcgcg
    18061 aagggaaaac ccaccagtta ccgcatgtta ttcaaaccgg gcagcaggtg gggatgataa
    18121 cgtttcagca gagttatcag cagcgggtgg gggaagggcg tttgtgacgt tgccatgccc
    18181 gatgtgcaag cttatcgggc ctacaaaggc aataaacgtc tgatgcgacg cttgcgcgtc
    18241 ttatcagacc tacaaccgct gccacatgta ggccggataa ggcgttcacg ccgcatccgg
    18301 caataaggcg taaggcgcga tgatattatt tacactccag caggcgttta aatcgcctta
    18361 atatccctgc tcgaaatagc tttcgagaat aataaccgca gaggcagagt cgactttgcc
    18421 tttgttgagc gcccgatagc cgccctgttc aaacagaccg gaacgggctt ccacggtgct
    18481 aagacgttcg tcatggagct ttacttcaac accgaaacga ccatgaatac ggttggcaaa
    18541 tttacgcgct ctggcagtca atggttgctc ggtaccgtcc atattcagcg gcaaaccgac
    18601 gatgatttcg tccggctgcc actctttcag taaacgttcg atgaggttcc agtccggcgt
    18661 accgtcctgt gctttaattg caggcaaagg gcgagcggtg ccggtaatgc gttggccgac
    18721 cgctacgcca atgcttttgg tgccgaagtc gaaggcgagt aaggttccac tcatcaggcg
    18781 tgccccgcaa cgccaggcat ggtgagaata tccacaccaa tcagttttgc cgcctcgcgc
    18841 cagcggtcgg caatcggcgt tttgaacaga atattcagat ctgccggggc cgttagccac
    18901 gcgttatcga gaatttcttg ttccagttga cctttctccc aggaggcata acccagagcc
    18961 accaatacgt cagacggttg tttatcggtg ccgagcgttt ccagcacatc gcgggaagtg
    19021 gtcatcactg tgttgtctga aatgcgaatg ctggaagcaa aattagaggg cggagtatgc
    19081 aaaataaacc cgcgatcttc agccagtggg ccgccgagca taaccggttt atccagacgg
    19141 attgattcat cacgcggctc cggcgtgatc ttcagctttt ccagaatccc ttcaattttg
    19201 agattttcca gcggcttgtt gacgatgatc cccattgcac cattggtatt atgttcgcaa
    19261 atgtagacca cggaacgacg gaaaatcgga tcctggagag caggcatggc aataagaaag
    19321 tgatgctgta aattcattgt cagaggttct gtttcctggt tcaaaaaagc aacagcgccc
    19381 agtatgcgtg gaaagaaggg cgctgtcact cagagtctca acgagatcct tctcgctaag
    19441 gtgggttact gctgctgtaa acgtgcttcg atggcatcca ttaacattcc ggtgatcgac
    19501 accggaaact ctgcttcaat ctcacgaata caggttgggc tggtgacgtt aatttcagtc
    19561 agacggtcgc cgatgatatc cagaccaaca aaaatcagcc ctttttcttt cagcgtcggc
    19621 ccgatctgac gggcgatttt ccagtcactt tccgtcagcg gacgaggttc accgcgacca
    19681 ccggcagcca gattaccacg ggtttcgccc ccctgcggaa tacgcgccag gcagtacggt
    19741 accggctcgc catccaccac cagcacgcgt ttgtcgccat ctttaatggc tggcaggtaa
    19801 ttttgtgcca tgcagtagcg agtgccatgc tcagtcaggg tttcggcaat cacaccgagg
    19861 tttggatcgc cttctttcac gcggaaaatc gacgcgccgc ccataccgtc cagcggctta
    19921 agaatgatgt cgctgtgttt ctcccagaac gcttttagct gcgctttatt gcgcgtaacc
    19981 agcgtttctg gcgttaagtc agagaaccag gcggtaaaca gtttctcgtt acagtcgcgc
    20041 aggctctgcg gcttgttaac gatcagcgtc cctttctctt cggcacgttc cagaatatag
    20101 gtcgcgtaga taaactcggt atcaaacggc gggtctttac gcatcaggat cacatcgaga
    20161 tcggccagcg gcagatcctg ttcaccgacg aatgaaaacc actcttcgta gttctgcttc
    20221 acgttcagcg tgcgggtatg ggcgcgggct tcgccattgc tcagatacag atcgcccatc
    20281 tccatatagt gaagttcgta accacgacgc tgtgcttcca gcaacatagc gaaactggaa
    20341 tctttcttga tgttgatgtt tgcgatgggg tccatcacga tgccgagctt gatcattatt
    20401 cttctccgtt agcccaaatc gccaaatcgt acttgtagcg cggtaatggc ggtgagcgca
    20461 gttgtctctg tacgcaaaac gcgaggtccc aacaggatat cagtaaattg atagcgggca
    20521 gtcatggcaa tttcatctgc cgataaaccg ccttccgggc caatcagcag gcggacgcgt
    20581 tcaaccggta aaggcaacgt attgatgctg ttactggcgc gcgggtgaag attcagtttc
    20641 agtccttcat cctgctctgc acaccaggct tccagatcca tcgctggacg gatttccggc
    20701 acccggttac gaccacactg ctcacaggca gcaattgcaa tcttctgcca ctgctggagc
    20761 ttcttgttca gacgttcact atccagttta acgccgcagc gctcagaaaa aagtggcgta
    20821 atgaggctta caccgagttc gatcgatttc tggatcgtaa attccatttt ttcaccacgc
    20881 gacatcacct gaccgaggtg aatatgcagc ggagattcgc gatcgtcgat ctggccttcc
    20941 agcaccttca cttccacgct ttttttgctg gcgctggtaa tttcggcgtc aaagacctgg
    21001 ttgctaccgt caaacaattg caacgcctgc cccggcccca tgcgcagtac gcgcccgata
    21061 tggttggcgg catcttcgca aagcgcgatg tgagaatggc tggtcagtgg ttcaggatga
    21121 taaatgcggg ggatacgcat agttaaaaat ccgcgtcgtc tccccacgcg gttgttcgcg
    21181 tggggtaggg gttaacaaaa agaatcccgc tagtgtaggt tagctctttc gcgcctggca
    21241 agcgcgttgc acatacgggt tatgattgcc ctgcaccttc gcgatgcgtt catcgcgctc
    21301 gcactcccag tcggtaaccg gatacatctt gttccatgcg ttgaacagct gcgtttgctg
    21361 gcgagagagt gtcaggttgt attggtcgcg catatagaag taggtgcgcg caatggcacc
    21421 gcgtgcacgc gctggtggtt cggcagcttt ttctttgaaa tcaaccttca tgacgcattg
    21481 accgtactgg ccttcgccgc cattccactg gctgtacata aagttgccgc gatcgccatt
    21541 cacctcgccg actgacggct gcaggttatg catatcgctt tccatcttgc gatagaccgg
    21601 atctttagcg cagtttttac gtccaccgtc ctgccagcac tggcgctggt gaccgaattg
    21661 ccaggcggga acgacgtgtt cccactctac gcggctggcg cggttttcat ttttgcgcac
    21721 ctgatagccg cacgattgca gatcaacaac gccttttttg ccctgccagt taattttaca
    21781 tccgcaataa aacgtaccgg gcgcgtcagc gtggactttt accgccgcgg ctttcgcctg
    21841 agaaaaacta ttgatacctt cggccaacgc cgggccggaa aatgctgcgc tcagtaccac
    21901 cgcagcaata gacaaataac ggtacatcac ttactccgtg ttaaaacgag ccagcaacgt
    21961 agcgaaagtt gttttggtat gcaatcagcc agatcaggaa agttcctgat agttcaggtg
    22021 gttatttcgc aaccagttgt tcgccgcagt gaacacagcg atagacggct tcgccacgaa
    22081 ctacgcgatt atggcggcgt acggtaagct gatgctcctg gcacttgcag cggtagggga
    22141 aggtgttgcg acgcacggat tgcagttcga actgatgcgt acgacgggcg ggaacaccca
    22201 gcacgttttc catcatccac ttccactctt tgccatgtgg tgctacgcgg ccgaaatgtt
    22261 tccataccag caaatgtgcc agttcgtgcg gcaccacttc ttcaataaag gcttcactgt
    22321 tttccaacag caaaacggga ttgaggcgga tttcatagct ttccagccag gctgttccgg
    22381 cggaggtgcc gcgctgggtg taagagagtt ttggctccgg gtagttacgc cccaatttca
    22441 ggttagcctg ggcgagtttt tcccgcaggc gacgcataac ggcctgttgg atggcgatag
    22501 ggagacggga tgttttcata gcggcagagg atagagcgaa gaaaagatga ctgcaagagg
    22561 cggcttcctc cgcgacggga ggaagcctga ggagattaat cgtgagcgcc gatttcgcgc
    22621 agtttacgac ctttcatcag attacgttca atatgttcca gcgaaacgtg tttggtttcc
    22681 ggtaccagcc acaatgtcag caggataaac agtacgttca gagccgcgta cacccagaag
    22741 gtgttggcgt tacccagcgt gttgagcatg gtcaggaacg ttgcgccaac gatcatgttg
    22801 gcaatccagt tggtggcggt ggagcaggtg atgccaaaat cgcggccttt cagcggctga
    22861 atttcggagc acagtaccca aatcagcgga ccggcactca tggcaaaacc gacaataaac
    22921 atcagcagca tggcgatggc gaaatactgc gccgacggag agtggatacc gatatgcatc
    22981 attgtaccga gtacgcccat accagcagcc atcaccagga agcccagcgt tagcgttggt
    23041 ttacgtcccc agcggtcaac aaggccgatt gcgataaagg tggcaagtac gttggtcagg
    23101 ccgacaatca ctgtccccca catttgctcg gtagtgttgg tataacccgc cagttcgaag
    23161 attttcggcg cgtaatacat gatgacgttc atcccggtga attgctgcat tacctgtaac
    23221 agtacgccaa ggaacaccgc gcggcggaag ttgctattct ctttaaacag cgcccagcca
    23281 ctctgtttaa cctgcaaact ttcacggatt tcatccagtt cgcgtttcgc ttccgcgctg
    23341 gtgtcacgca ggcgtagcag cacgcgttcg gcatcaacaa aacggcgttt ggcggcaaac
    23401 caacgtgggc tgtctggcag gaagaagaca ccaatcagca gcaaaattgc cgggatgata
    23461 atcacaccca gcatccagcg ccatgcaccg gtgtagctga aggcggtatc agaaagataa
    23521 gcaccgagga tcccgatagt gatcatcaac tgatacatcg agatcatact gccgcgaatt
    23581 ttttccggcg caatttcaga gaggtacagt ggtgcggtat aagaggccac acccaccgcc
    23641 agccccagca gaacgcggga aagaatcagt acttcaacgt ttggcgcagc cgcagagaac
    23701 agcgaaccgg caacaaacaa aattgcgccg atcatcaggc tctttttgcg tccgagttta
    23761 aaggagagcc agccgctgcc caccgcaccg actgccgcac cgaacatcat ggagcttacg
    23821 acccattctt gcgtgtgcga agtaatctgg aattcatctg caataaacgg cagtgcgcca
    23881 gcaattacac cgatatccag gccaaagagt aatcccgcca gagcggcaag gaagcagacg
    23941 aaaaacgtca ttgccttgtt tgaccgcccc tgttttttag cgtcaggcat gatgccctcc
    24001 aatatggtta ttttttattg tgaattaaga taggtgagta cgacataaaa agatgtgaag
    24061 caaatcacat cagtgtaatc gcttacacta acatttatct tagttatgat ttaagtgcat
    24121 gaaaataatt gagttatatc atcatagatt aaacgctgtt atctgcaatt aagactttac
    24181 tgaaaagaaa tgtaacaact gtgaaaaccg aataataata tgaaaatatt acaaatcttt
    24241 gtctgttgtg gttggtgtaa tcggtttcag ttgtgatgat aatttgcggt gaaacgtgat
    24301 caggaagaat gatgttattg tgcgggcagt ttttttgccg gatgcggcgt gaacgcctat
    24361 ccaccgcggt gaggtaaaag aaaaaggcca gcgcgaggct ggcctttgaa cgcaggtgaa
    24421 gaaagattac ttcagaccgg cagcatcgcg cagcagctgc gctttgtcgg ttttttccca
    24481 cgggaaatgt tcacgaccaa agtgaccgta tgctgcggtt tctttgtaga tcgggtgcag
    24541 cagatccagc atctgaatca gaccgtatgg gcgcaggtcg aagaactcac gtaccagcag
    24601 agtcagttgt tcagaaggca ctttctcagt accgaaagtt tctaccatga tggaagtcgg
    24661 ttcagccacg ccgattgcgt aggaaacctg aatttcacaa cgatcggcca ggccagcagc
    24721 aacgatgttt tttgcaacat aacgtgctgc gtacgcagcg gaacggtcca cttttgatgg
    24781 atctttacca gagaatgctc cgccaccgtg acgtgccatg ccgccgtagg tatcaacgat
    24841 aattttacga ccagtcagac cgcagtcacc cattgggcca ccgataacga aacgaccggt
    24901 cgggttgatg aagaatttgg tggcagaagt cagccattca gcgggcagaa ttggcttgat
    24961 gatctcttcc attaccgctt cttgcagcga tttctggtcg atctcttcag agtgctgagt
    25021 ggaaagcacg acagcatcaa taccaacgat tttgccgtcg tcatactgga aagtcacctg
    25081 gcttttcgcg tccgggcgca gccacggcag agtgccattt ttacgcactt cagcctgacg
    25141 ctgtaccaga cggtgtgcat aggtgatagg tgctggcatc agcacgtcgg tttcattagt
    25201 tgcgtagcca aacatcagac cctggtcacc cgcgccctgt tccagcggat cggcacggtc
    25261 aacgccctgg ttgatgtcag gagactgttt gccgatagcg ctcagaaccg cacaggagtt
    25321 agcgtcaaag cccatgtcgg aatgcacata gccaatttcg cgaacggtgt tacgggtgat
    25381 ctcttcgatg tctacccagg cgctggtggt gatttcgccg ccaactaaaa ccatgccggt
    25441 ttttacgtag gtttcgcaag caacgcgtgc tttcggatcc tgttcgagga tcgcgtctaa
    25501 aacggcatca gagatttggt cagcaatttt gtcaggatgc ccttcagaga cggactcgga
    25561 cgtaaaaagg tgttttgcca tatttaatat cacctaaaga gaatttggtt agctcaaact
    25621 gttgtgtgga ttttctgtgg tagcggatcc taccacgact ctgcaggtta aaaacactgg
    25681 cagtctgagt gttaatcggt atggatggat taacatctgg atggctattt taggtcaatt
    25741 cttcacccga tttccacttt tttttgaatc gtgtctcatt ctgttaaaaa cgtggctgga
    25801 aatttttcct gacaatgccg gcattctgcg tatttatctt ttgcaatttt ctgccattgt
    25861 ggggtataaa acgcggcgcg cggcttaaat aaaaagcaca cgacgtttct ttcgtgttgc
    25921 cacttccagc cgggttgaaa tcagagtttt ggcttgtggg ttcgtcttaa caggcggccg
    25981 tggaggtgat acgaaataat gaaccgttgt ctgctgctta acctgtctca ccgttctggt
    26041 gaagattcgt tccccgcact ctgcatctct gctttgcata cctgccgatg ttatacccat
    26101 ctcggcgctt ctcaggattc aagagctggt tacagttact gaggactgaa caagggcgct
    26161 cttgtaaaaa caagagtttt ctcgtggttt cgccgaactg tcacacttac gttcggttat
    26221 gtgcttaata atgttatgaa aaagaaaccg gttgcgcagt tggagcgtca gcattcactg
    26281 ctggaaaatc catgtgctta tgggttgtta tcgcagttcc aggctgcgat agtcgttaac
    26341 tgttttacac ttaataaaat aatttgaggt tcgctatgtc tgacgacatg tctatgggtt
    26401 tgccttcgtc agcgggcgaa cacggtgtac tacgctccat gcaggaggtt gcaatgagct
    26461 cccaggaagc cagcaagatg ctgcgtactt acaatattgc ctggtggggc aataactact
    26521 atgacgttaa cgagctgggc cacatcagcg tgtgcccgga cccggacgtc ccggaagctc
    26581 gcgtcgatct cgcgcagtta gtgaaaactc gtgaagcaca gggtcagcgt ctgcctgcac
    26641 tgttctgttt cccacagatc ctgcagcacc gtttgcgttc cattaacgcc gcgttcaaac
    26701 gtgcgcggga atcctacggc tataacggcg attacttcct tgtttatccg atcaaagtta
    26761 accagcaccg tcgcgtgatt gagtccctga ttcattcggg cgaaccgctg ggtctggaag
    26821 ccggttccaa agccgagttg atggcagtgc tggcacatgc tggcatgacc cgtagcgtca
    26881 tcgtttgcaa cggttataaa gaccgcgaat atatccgcct ggcattaatt ggcgagaaga
    26941 tggggcacaa ggtctatctg gtcattgaga agatgtcaga aatcgccatt gtgctggatg
    27001 aagcagaacg tctgaatgtc gttcctcgtc tgggcgtgcg tgcacgtctg gcttcgcagg
    27061 gctcgggtaa atggcagtcc tccggcgggg aaaaatcgaa gttcggcctg gctgcgactc
    27121 aggtactgca actggttgaa accctgcgtg aagccgggcg tctcgacagc ctgcaactac
    27181 tgcacttcca cctcggttcg cagatggcga atattcgcga tatcgccaca ggcgttcgtg
    27241 aatcggcgcg tttctatgtg gaactgcaca agctgggcgt caatattcag tgcttcgacg
    27301 tcggcggcgg tctgggcgtg gattatgaag gtactcgttc gcagtccgac tgttcggtaa
    27361 actacggcct caatgaatat gccaacaaca tcatctgggc gattggtgat gcgtgtgaag
    27421 aaaacagtct gccgcatccg acggtaatca ctgaatcggg tcgtgcagtg actgcgcatc
    27481 acaccgtgct ggtgtctaat atcatcggcg tggaacgtaa cgaatacacg gtgccgaccg
    27541 cgcctgcaga agatgcgccg cgcgcgctgc aaagcatgtg ggaaacctgg caggagatgc
    27601 acgaaccggg aactcgccgt tctctgcgtg aatggttaca cgacagccag atggatctgc
    27661 acgacattca tatcggctac tcttccggca cctttagcct gcaagaacgt gcatgggctg
    27721 agcagcttta tttgagcatg tgccatgaag tgcaaaagca gctggatccg caaaaccgtg
    27781 ctcatcgtcc gattatcgac gagctgcagg aacgtatggc ggacaaaatg tacgtcaact
    27841 tctcgctgtt ccagtcgatg ccggatgcgt gggggatcga ccagttgttc ccggttctgc
    27901 cgctggaagg gctggatcaa gtgccggaac gccgcgctgt gctgctggat attacctgtg
    27961 actctgacgg tgctatcgac cactatatcg atggtgatgg tattgccacg acaatgccta
    28021 tgccggagta cgatccagag aatccgccga tgctcggttt ctttatggtc ggcgcatatc
    28081 aggagatcct cggcaacatg cacaacctgt tcggtgatac cgaagcggtt gacgtgttcg
    28141 tcttccctga cggtagtgta gaagtggaac tgtctgacga aggcgatacc gtggcggaca
    28201 tgctgcaata tgtacagctc gatccgaaaa cgctgttaac tcagttccgt gatcaagtga
    28261 agaaaaccga tcttgatgct gaactgcaac aacagttcct tgaagagttc gaggcaggtt
    28321 tgtacggtt
//
LOCUS       DAZUGK010000039        28321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZUGK010000039 DAZUGK010000000
VERSION     DAZUGK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.039"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            205..1482
                     /gene="waaA"
                     /locus_tag="ACHY5W_001507"
     CDS             205..1482
                     /gene="waaA"
                     /locus_tag="ACHY5W_001507"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFS4446429.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1490..1969
                     /gene="coaD"
                     /locus_tag="ACHY5W_001508"
     CDS             1490..1969
                     /gene="coaD"
                     /locus_tag="ACHY5W_001508"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4446430.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(2008..2817)
                     /gene="mutM"
                     /locus_tag="ACHY5W_001509"
     CDS             complement(2008..2817)
                     /gene="mutM"
                     /locus_tag="ACHY5W_001509"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFS4446431.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTNELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2915..3082)
                     /gene="rpmG"
                     /locus_tag="ACHY5W_001510"
     CDS             complement(2915..3082)
                     /gene="rpmG"
                     /locus_tag="ACHY5W_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4446432.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3103..3339)
                     /gene="rpmB"
                     /locus_tag="ACHY5W_001511"
     CDS             complement(3103..3339)
                     /gene="rpmB"
                     /locus_tag="ACHY5W_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4446433.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3556..4224)
                     /gene="radC"
                     /locus_tag="ACHY5W_001512"
     CDS             complement(3556..4224)
                     /gene="radC"
                     /locus_tag="ACHY5W_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4446434.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4396..5616
                     /gene="coaBC"
                     /locus_tag="ACHY5W_001513"
     CDS             4396..5616
                     /gene="coaBC"
                     /locus_tag="ACHY5W_001513"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFS4446435.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVIPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5594..6052
                     /gene="dut"
                     /locus_tag="ACHY5W_001514"
     CDS             5594..6052
                     /gene="dut"
                     /locus_tag="ACHY5W_001514"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4446436.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6159..6755
                     /gene="slmA"
                     /locus_tag="ACHY5W_001515"
     CDS             6159..6755
                     /gene="slmA"
                     /locus_tag="ACHY5W_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFS4446437.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6792..7433)
                     /gene="pyrE"
                     /locus_tag="ACHY5W_001516"
     CDS             complement(6792..7433)
                     /gene="pyrE"
                     /locus_tag="ACHY5W_001516"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4446438.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7499..8215)
                     /gene="rph"
                     /locus_tag="ACHY5W_001517"
     CDS             complement(7499..8215)
                     /gene="rph"
                     /locus_tag="ACHY5W_001517"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4446439.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8342..9205
                     /gene="yicC"
                     /locus_tag="ACHY5W_001518"
     CDS             8342..9205
                     /gene="yicC"
                     /locus_tag="ACHY5W_001518"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4446440.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9425..10247
                     /gene="dinD"
                     /locus_tag="ACHY5W_001519"
                     /pseudo
     CDS             9425..10247
                     /gene="dinD"
                     /locus_tag="ACHY5W_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            10538..11155
                     /gene="yicG"
                     /locus_tag="ACHY5W_001520"
     CDS             10538..11155
                     /gene="yicG"
                     /locus_tag="ACHY5W_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFS4446441.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFCARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11152..12834)
                     /gene="ligB"
                     /locus_tag="ACHY5W_001521"
     CDS             complement(11152..12834)
                     /gene="ligB"
                     /locus_tag="ACHY5W_001521"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFS4446442.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNQARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLVSTEQFWQQLPGIGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            13092..13715
                     /gene="gmk"
                     /locus_tag="ACHY5W_001522"
     CDS             13092..13715
                     /gene="gmk"
                     /locus_tag="ACHY5W_001522"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4446443.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13770..14045
                     /gene="rpoZ"
                     /locus_tag="ACHY5W_001523"
     CDS             13770..14045
                     /gene="rpoZ"
                     /locus_tag="ACHY5W_001523"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4446444.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            14064..16172
                     /gene="spoT"
                     /locus_tag="ACHY5W_001524"
     CDS             14064..16172
                     /gene="spoT"
                     /locus_tag="ACHY5W_001524"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4446445.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGDGSIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16179..16868
                     /gene="trmH"
                     /locus_tag="ACHY5W_001525"
     CDS             16179..16868
                     /gene="trmH"
                     /locus_tag="ACHY5W_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFS4446446.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPDSRMRTMASAAAGSNSWVQVKTHRTIGDAVTHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16874..18955
                     /gene="recG"
                     /locus_tag="ACHY5W_001526"
     CDS             16874..18955
                     /gene="recG"
                     /locus_tag="ACHY5W_001526"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4446447.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPPQAKALIER
                     WMPETERYSNA"
     gene            complement(18940..19806)
                     /locus_tag="ACHY5W_001527"
     CDS             complement(18940..19806)
                     /locus_tag="ACHY5W_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446448.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(19809..21014)
                     /gene="gltS"
                     /locus_tag="ACHY5W_001528"
     CDS             complement(19809..21014)
                     /gene="gltS"
                     /locus_tag="ACHY5W_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFS4446449.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            21294..22685
                     /gene="xanP"
                     /locus_tag="ACHY5W_001529"
     CDS             21294..22685
                     /gene="xanP"
                     /locus_tag="ACHY5W_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFS4446450.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            22806..24515
                     /gene="yicH"
                     /locus_tag="ACHY5W_001530"
     CDS             22806..24515
                     /gene="yicH"
                     /locus_tag="ACHY5W_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS4446451.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(24568..26886)
                     /gene="yicI"
                     /locus_tag="ACHY5W_001531"
     CDS             complement(24568..26886)
                     /gene="yicI"
                     /locus_tag="ACHY5W_001531"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFS4446452.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(26896..28278)
                     /gene="yicJ"
                     /locus_tag="ACHY5W_001532"
     CDS             complement(26896..28278)
                     /gene="yicJ"
                     /locus_tag="ACHY5W_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFS4446453.1"
                     /translation="MMSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTIKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
BASE COUNT         6903 a         7697 c         7146 g         6575 t
ORIGIN      
        1 ccatattctt tcactgttgc tctgcgattc cttcctgcac gtacagcaga aatcaatgaa
       61 tcgcataaaa atacaaaaaa agtatagatg ttcagcaaga attggcgggt aaattgctat
      121 ttaagtcaaa aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc
      181 acatccataa gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt
      241 attcagccgc tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga
      301 aaacgctggg gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg
      361 ctgcactccg tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt
      421 catcgttatc ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc
      481 gtacaatcgg ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat
      541 gcacttaatc gtttcctgaa taaagtcgac cctaaactgg tgttgattat ggaaaccgaa
      601 ctatggccta acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac
      661 gcgcgactct ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgtcgc
      721 ttgctgcgtc gtattacgct gattgccgcc caaaatgaag aagatggtgc acgttttgtg
      781 gcgctgggcg caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct
      841 gtaacgccgc agttggctgc taaagccgtg acgctgcgcc gccaatgggc accacaccgc
      901 ccggtatgga ttgccaccag cactcacgaa ggcgaagaga gtgtggtgat cgccgcacat
      961 caggcattgt tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccggaa
     1021 cgcttcccgg atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctcc
     1081 tcaggggaag ttccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg
     1141 atgttactgt atggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt
     1201 gggcataatc cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact
     1261 tttaacttta aagacatttg cgcgcggctg gagcaggcaa gcgggctgat taccgttacc
     1321 gatgccacgt cgcttgcaaa agaggtttcc tctttactca ccgacgccga ttaccgtagt
     1381 ttctatggcc gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg
     1441 cttcaactgc tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg
     1501 ggcgatttat ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg
     1561 cgccacgcaa atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc
     1621 gatgtttacc ctggaagagc gtgttgagct ggcacagcag gcaaccgcgc atctggggaa
     1681 cgtggaagtg gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac
     1741 ggtgctgatt cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca
     1801 tatgaatcgc cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg
     1861 gtcgtttatc tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca
     1921 tttcctgccg gagaatgtcc atcaggcgct gatggcgaag ttagcgtagc gtttatgccg
     1981 gatggtatgc catccggcgc gcatgaatta cttctggcac tgccgacaat aaaacgttgc
     2041 ccgctgcgca tgtttagtcg ccacaatcgg cgtaccgcac acccgacagg gctcgccttt
     2101 tcgcccgtaa acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa
     2161 atctttcagc gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg
     2221 agctaacaat tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc
     2281 cgccgcaaac agagattcgc tggcatagat attccctacc cctaccacca gcttgttatc
     2341 catcagccac ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca ggtactcacc
     2401 gttgaaatcg tcgctaagcg gctccggtcc aagatgagcc agcacgttgt gcccttccag
     2461 ctcgttggtc cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc
     2521 gttgctcatc accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat
     2581 gcgcaggctg ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag
     2641 cagatattta gcccgccgct gcacgctaag cactggttgg tcgcttaaac ggtagatctc
     2701 ttctgaaacc ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc
     2761 aacgagatgc ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc
     2821 atctccagga atgaacagat gcagtcaata tgggggcaag cagataacaa aaaaccccgc
     2881 cggagcgagg ttttttgtta catcaaagcg agaattattt gattttcgct tctttgtaga
     2941 tcacgtgctg gcgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac
     3001 gtttgttctt cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt
     3061 tctcacgaat acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg
     3121 ggcacgcagt tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt
     3181 agcagatacg cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg
     3241 caggttcggc aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt
     3301 caccggacgc ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa
     3361 ttagctcgag cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc
     3421 agttctatgt gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg
     3481 gcgtagtata cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg
     3541 atcgcgcata gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc
     3601 aatcacgata tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc
     3661 agtaatgagt ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag
     3721 gatcagcgcc gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc
     3781 aacatggttt agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc
     3841 gagaaagatc accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc
     3901 ccgcgtcatc tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg
     3961 ccgcgccagt tcagcaatcc cctttaactg ggcaaatttc gccacgccaa ttccatgaac
     4021 accactaaat tgttcatatt cagaggttaa caagccataa agagagccga aattctccag
     4081 catctctttt gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc
     4141 cagcagctcg acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat
     4201 caacagctgt gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag
     4261 ccgttccgtt aaatcgacgg tcagttttca atcctggaaa gcgcctcgca aagtgaatct
     4321 caggtgaagg cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccct
     4381 acaggaaaaa tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt
     4441 attgctgcct ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc
     4501 cgcgtagcca tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt
     4561 tctggttatc ccgtttccga cagtctgctg gacccggcag ccgaagccgc tatgggccat
     4621 attgagctgg gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttaatt
     4681 gcccgtgttg ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct
     4741 gcgcctgtag ccgtgatccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag
     4801 cataatttag aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt
     4861 caggcttgtg gtgatatcgg tcctgggcga atgctcgatc cgttaaccat tgtggatatg
     4921 gcagtagcgc atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc
     4981 ggcccgacgc gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag
     5041 atgggttttg ctatcgccgc cgccgctgcc cgtcgtggcg cgaacgtcac gctggtatca
     5101 ggtccggttt cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gactgcgctg
     5161 gaaatggaag ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggctgcgcc
     5221 gccgtggcgg attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg
     5281 cagggtgatg aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc
     5341 gcactaaaag accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa
     5401 gaatacgccc ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt
     5461 tcccagccaa ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac
     5521 ggagataaag tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag
     5581 atcgtgaccc gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttgggaa
     5641 ggaatttccg ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg
     5701 tctcgacgac gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc
     5761 gattcatatt gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca
     5821 taagcacggt atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca
     5881 gttgatgatt tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg
     5941 catcgcccag atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt
     6001 cgacgccacc gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg
     6061 catccgaata acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc
     6121 cgcctggcaa gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga
     6181 aaaggaaccg tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg
     6241 gaagccaacg tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac
     6301 tgtatcgcca cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag
     6361 atagcctgat tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc
     6421 tgcgtctgat tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca
     6481 tcctcactgg tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc
     6541 tgttcgagcg tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg
     6601 gtgaaggtta caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag
     6661 gtatgctgtc acgctttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg
     6721 cccgctggcc gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc
     6781 ggcgagtttc tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc
     6841 gccatttccg gcttctcttc caggtaggca atcagatctt tcagggtgat gatagagatc
     6901 actttgcagt tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt
     6961 tcctgacgat caagcgaaat caatacgcca gcaagcgtcg cgccattggc ctgaataatt
     7021 tccatcgact cgcgaatcgc cgttccggcg gtgatcacat catccaccag cattacgcgt
     7081 ccttgtaacg cgctaccgac cagattgccg ccttcaccgt ggtcttttgc ctctttgcgg
     7141 ttaaagcagt acggcaggtc caggtcatga tgctccgcca gcgccacggc ggttgtggta
     7201 gcaatcggga tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc
     7261 accaatgctt cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc
     7321 ccggcgttga agaaataggg gcttttgcgc ccggatttca gcgtaaactc accaaacttt
     7381 aacacctgct tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg
     7441 ctcctcatct tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc
     7501 agtttgccag cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg
     7561 ccaacaaggt gagtaactct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt
     7621 caatgatgcg cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt
     7681 caacgtattc cagatcgcaa accgcttcgc cgttcacaat tccgacagaa actgcggcta
     7741 ccatcccttt catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat
     7801 ctgccagcgc cacgcaggca cccgtaatcg acgcggtacg cgtgccacca tcagcctgaa
     7861 gcacgtcgca gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc
     7921 gaagagcacg ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg
     7981 ccgcttcacg agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga
     8041 tccagccctg gccctgacct ttcaggaagc gaggcacgcc ttcttcaata gaggcggtac
     8101 acaacacttt ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt
     8161 tacgagtcag ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga
     8221 aatctccggc ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta
     8281 tcctgacaag gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc
     8341 tatgatccgc agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc
     8401 aacctgggaa atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga
     8461 gcagttccgt agccttgaac ctgtcgttcg tgagcgtatt cgttctcgcc tgacgcgcgg
     8521 taaagtggaa tgtaccctgc gctatgagcc agatgttagc gcgcaaggtg agctgatcct
     8581 caacgaaaaa ctggctaaac agctggtaac tgccgcaaac tgggtaaaaa tgcagagtga
     8641 cgaaggggaa atcaacccgg ttgatattct gcgctggccg ggcgtgatgg cagcccagga
     8701 gcaggatctt gacgccattg ccgctgaaat tctcgcggcg ttggatggca ccctggacga
     8761 ctttattgtc gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct
     8821 ggaaggcgtc accgccgaag tggtcaaagt ccgctcccat atgccggaaa tcctgcaatg
     8881 gcagcgtgaa cgcttggtcg cgaagctgga agatgctcag gtgcagttgg aaaacaaccg
     8941 tctggagcag gagttggttc tgctggcaca acgaattgac gttgccgaag aactggaccg
     9001 cctcgaagcg catgtcaaag agacctacaa cattctgaag aaaaaagaag ccgttggtcg
     9061 tcgtctggac tttatgatgc aggagttcaa ccgcgagtcg aacactcttg cgtcgaaatc
     9121 tatcaacgcc gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg
     9181 cgagcagatt cagaacatcg aataactcgt aacgccaatt cttacttttc cgccttcaca
     9241 aatgccgcca ctcaaacaga gcggcatttt tcttccccgc aacattcaat tctgttttgc
     9301 gtgcctgctc cagattttgc gatgtttttt gcccagcact ctgagaacgt gagatactca
     9361 caactgtata taaatacagt tgcagattta ctttctttgc aatcgatatc acatggagtg
     9421 ggcaatgaac gaacatcatc aaccttttga agagatacgg cattatggca ctgaagggca
     9481 agaattttgg tcagcgagag aattggcacc gttactggat tatcgggact ggcgcagttt
     9541 tcaaaaggta ctgacccgcg cgactcaagc gtgcgaagcc agcaatcaag ctgcatctga
     9601 ccatttcgtt gaaaccacca aaatggttgt attaggttca gggctcaacg agaacttgaa
     9661 gatgttcatc tctcccgcta tgcctgttac ctggtagtac aaaacggcga ccctgcgaaa
     9721 ccggttattg cggcaggaca aacttatttt gctatccaga cccgacggca ggagctggct
     9781 gacgatgaag ctttcaggca acttcgtgaa gacgaaaagc gtctttttct gagaaatgaa
     9841 ttgaaagaac ataacaaaca attagttgag gcagcgcagc aagctggggt agcaacagct
     9901 atcgatttcg ccatatttca gaatcatggt taccggggct gtatggtgga ttagatcaga
     9961 aagctattta tcagcggaag gggctgaaaa agggtcagaa gatcctggat catatgggtt
    10021 caacagaact ggcggctaat ctctttcgag ctacccaaac agaagaaaaa ctcaagcggg
    10081 atggcgtgaa ttcaaaacag caagctaata ccacacactt tgacgtgggt agcaaggtga
    10141 ggcaaaccat tcaggaactt ggcggaacca tgcctgagga gttgccgacc ccgcaggtca
    10201 gcatcaagca gttggaaaac agtgtaaaaa ttacagagaa gaaatagcag aaactaaaaa
    10261 ctctttttgt tgattgagac acccgatgcg taagccaagg tccaggtgca gtgaacacaa
    10321 tggctaaata ttgcaccttt cttttccccc ctcagtttta acctattttt tcttatgcat
    10381 tttctcagac aagaagtcag aagaatgcat ctctgctaca gaaaatagcg atttcacata
    10441 actacaagtt atcaatttcc cctcacctta aaaaatctca atcgtgacaa tgcgcacaaa
    10501 tcgctaccct gccagacaga tttttaggga gagaaccatg ctgttacaca ttttgtattt
    10561 ggttggcatt actgccgaag ccatgacggg ggctctggcg gccgggcgac gccgcatgga
    10621 tacatttggc gtaattatta ttgcgaccgc caccgcaatt ggcggagggt cagtgcgcga
    10681 tattctgctg ggccactatc cgcttggctg ggtcaaacac ccggaatatg tgattatcgt
    10741 cgctaccgcc gcagtactca ccactatcgt tgctcctgta atgccttacc tgcgcaaagt
    10801 gtttctggtg ctcgatgcgc tcggactggt ggtcttttct atcattggcg cacaggttgc
    10861 actggatatg gggcacggcc caattattgc cgttgtcgcg gcggtaacca ccggcgtgtt
    10921 tggcggcgtt ttgcgcgata tgttctgtaa acgcatccca ctggtattcc agaaagagtt
    10981 gtatgccggt gtctcatttg cctccgccgt gctgtacatc gcgctgcaac actatgtttc
    11041 taaccatgat gtggtgatta tctccaccct ggtattcggc ttttgtgctc gtttactggc
    11101 gctacgtctt aagctgggat taccggtctt ttactacagc cacgaaggtc actaaggttc
    11161 aaaacctgtg atctgctggg cagccagcca actgcccagc ttcttgattt gcgcattttc
    11221 cttccattca ataacctgtc tggcgcgccc agatccaatc cccggcagct gctgccagaa
    11281 ctgttccgtg ctaactaaaa gttgcgacca ggaccgttca tcactggcat taagcgccgc
    11341 ccgggttagc ggtattccca ttgccatcac ccagcgagta aaaggctgct tacgagcctg
    11401 attaaactga tgccataact gcgcactttt acttttcgcg atccctggcg tgttctgtaa
    11461 ttgctctggc gttaataaaa gccaggaaaa gatatgctca aagcgatgag tctgatgcag
    11521 cgcgcgccaa ccggcctcac caatgccatc cagccccaga acctgttttg accccagcca
    11581 gactaagcgt gaaatgaact gttcctgaca aacatcagaa gcaaagtagc aggtcaacga
    11641 gttaaagcgg ttttctggtg gtgtcggttt tgtacgttct gcaccgcgcc agaccacatc
    11701 atcaatgcga ggaataccct gaccggcaag gctgacgagg atctgatcac caggtgcaat
    11761 atcccactcc tgccagcgcc tgacggaacc aatattcacc cgctggactt ttttatcatc
    11821 cagcatgaca ggtgcgagtg acgcaaccac cgatatttta ccgctcttac ccaccgcaaa
    11881 ctgaatcgcc ttcacttcgg caacctgagc tacaggttga tatttccagg ccaccagcca
    11941 ctccgcctgg cccggtagcc aatgacggga ttcgggttcc ttagcccctc gcacaaccac
    12001 gccatcggtg acgaagggta atttcgcttt ccaccactca ttgcgtacgc gcgcaacctc
    12061 atcagcattt ttaaccgcac gggtatacct ctgcgttaga gtaaaacctg cggtagccag
    12121 ctcctttaaa cgatcggtca ttaactgcgg tccatccggc catgcccaga caaaaactgc
    12181 cagtgagttt agcgtgtcgc tattcccctg gcgcatcatc aggccagcaa cttttgcgcg
    12241 ggcatttatt ccccccattt gttgttggat atgcccctcg cgctggagaa atatttcccc
    12301 ctgaagcgta ctgttggcta aaggcccgct aacggtttgc ggcacagcgg aaattaagct
    12361 aactttctgc gtccagtcct cgcctttcag gccattaccg cgactgattg ctttgttcag
    12421 tttcccgtcc cgataaacca gggttaccgc aacgccatca acttttggct gcacccaaag
    12481 atcgctacgt tctcgcatcc acagacttaa tgcgttttta tccaccattt tgcgtacacc
    12541 cgtatgggca accggatgca taaccgcacc atttaaaggc ggcatcatca cgtcgcgggg
    12601 ctcgctccca aagcagcgtt gccactgcgt aagacgggcg cttaactgat cgtaaacacc
    12661 gtcttccacc tcactttttc cttccttcca gtagtcatcg tcccactgtt ttatttgctg
    12721 ttgcaggcgg gaaatttctt cctgtgctct ggctggcgac caggccggac agaccgccca
    12781 cacagatgat tgccagcaca agatacttat taatatcgcc atccatactt tcatcatcac
    12841 ctccgctgta gatagtcagg cagatataca acgtgatgaa agcaaagccg agtggcaaaa
    12901 acggagtctg cgaggacgct tcctgaaaat cgtctttatt gcagtgaatg acaggcaaat
    12961 gcggaagcag ctacgcaaaa cgcaacaact ttgcgcaaaa agtgtgagca agggctacgt
    13021 cacatggccg cgccgtgtat aataagctcg tatgtaggct ttatttcgct aatcacatac
    13081 gaaagatact catggctcaa ggcacgcttt atattgtttc tgcccccagt ggcgcgggta
    13141 aatccagcct gattcaggct ttattaaaaa cccaaccgtt gtatgacacc caggtttctg
    13201 tttcacacac cacacgccaa ccgcgtcctg gtgaagtcca cggtgaacat tatttctttg
    13261 ttaatcatga tgaatttaaa gaaatgatta gcagagatgc gttcctcgaa cacgcagaag
    13321 tttttggtaa ttactatggc acttcgcgtg aggccattga gcaagtcctg gcgaccggtg
    13381 tcgatgtttt tctcgatatc gactggcagg gtgcgcagca aattcgccag aagatgccgc
    13441 acgcgcggag tatctttatt ttaccgccgt ccaaaattga actggaccgc cgtctacgcg
    13501 gtcgcggtca ggacagcgaa gaggtcattg caaagcgtat ggcgcaagct gttgcagaaa
    13561 tgagccatta cgccgaatat gattatctga ttgtgaatga tgacttcgat accgcgttga
    13621 ccgatttgaa gaccattatt cgcgccgaac gtctgcgcat gagccgccaa aagcagcgtc
    13681 atgacgcttt aatcagcaaa ttgttggcag actgaacctg atttcagtat catgcccagt
    13741 catttcttca cctgtggagc tttttaagta tggcacgcgt aactgttcag gacgctgtag
    13801 agaaaattgg taaccgtttt gacctggtac tggtcgccgc gcgtcgcgct cgtcagatgc
    13861 aggtaggcgg aaaggatccg ctggtaccgg aagaaaacga taaaaccact gtaatcgcgc
    13921 tgcgcgaaat cgaagaaggt ctgatcaaca accagatcct cgacgttcgc gaacgccagg
    13981 aacagcaaga gcaggaagcc gctgaattac aagccgttac cgctattgct gaaggtcgtc
    14041 gttaatcaca aagcgggtcg cccttgtatc tgtttgaaag cctgaatcaa ctgattcaaa
    14101 cctacctgcc ggaagaccaa atcaagcgtc tgcggcaggc gtatctcgtt gcacgtgatg
    14161 ctcacgaggg gcaaacacgt tcaagcggtg aaccctatat cacgcacccg gtagcggttg
    14221 cctgcattct ggccgagatg aaactcgact atgaaacgct gatggcggcg ctgctgcatg
    14281 acgtgattga agatactccc gccacctacc aggatatgga acagcttttt ggtaaaagcg
    14341 tcgccgagct ggtagagggg gtgtcgaaac ttgataaact caagttccgc gataagaaag
    14401 aggcgcaggc cgaaaacttt cgcaagatga ttatggcgat ggtgcaggat atccgcgtca
    14461 tcctcatcaa acttgccgac cgtacccaca acatgcgcac gctgggctca cttcgcccgg
    14521 acaaacgtcg ccgcatcgcc cgtgaaactc tcgaaattta cagcccgctg gcgcaccgtt
    14581 taggtatcca ccacattaaa accgaactcg aagagctggg ttttgaggcg ctgtatccca
    14641 accgttatcg cgtaatcaaa gaagtggtga aagccgcgcg cggcaaccgt aaagagatga
    14701 tccagaagat tctttctgaa atcgaagggc gtttgcagga agcgggaatt ccgtgccgcg
    14761 tcagtggtcg cgagaagcat ctttattcga tttactgcaa aatggtgctc aaagagcagc
    14821 gttttcactc aatcatggac atctacgctt tccgcgtgat cgtcaatgat tccgacacct
    14881 gttatcgcgt gctcgggcaa atgcatagcc tgtacaagcc gcgtccgggt cgcgtgaaag
    14941 actacatcgc cattccaaaa gcgaacggct atcagtcgtt gcacacctcg atgattggcc
    15001 cgcacggcgt gccggttgag gtccaaatcc gtaccgaaga tatggatcag atggcggaga
    15061 tgggtgttgc cgcgcactgg gcttataaag agcacggcga aaccagcacg actgcacaaa
    15121 tccgcgccca gcgctggatg caaagcctgc tggagctgca acaaagcgcc ggtagctcgt
    15181 ttgaatttat cgagagcgtt aaatccgatc tcttcccgga tgagatttac gttttcacac
    15241 cggaagggcg cattgtcgaa ctgcctgccg gtgcaacgcc tgtcgacttc gcttatgcag
    15301 tgcataccga tatcggtcat gcctgcgtgg gcgcacgcgt cgaccgtcag ccttacccgc
    15361 tgtcgcagcc gcttactagc ggtcagaccg ttgaaatcat taccgctccg ggcgctcgcc
    15421 cgaatgccgc ttggctgaac tttgtcgtta gctcgaaagc gcgcgccaaa attcgtcagt
    15481 tgctgaaaaa cctcaagcgt gatgattctg taagcctggg ccgtcgtctg ctcaaccatg
    15541 ctttgggtgg tagccgtaag ctgaatgaaa tcccgcagga aaatattcag cgcgagctgg
    15601 atcgcatgaa gctggcaacg cttgacgatc tgctggcaga aattggtctt ggcaacgcga
    15661 tgagcgtggt ggtcgcgaag aatctccagc agggagacgg ttccattcca ccggcgactc
    15721 aaagccacgg gcatctgccg atcaaaggcg ctgatggcgt gctgatcacc tttgcgaaat
    15781 gctgccgccc cattcctggc gacccgatta tcgcccatgt cagccccggt aaaggtctgg
    15841 tgatccacca tgaatcctgc cgtaacatcc gtggctacca gaaagagcca gagaagttta
    15901 tggctgtgga atgggataaa gagacggcgc aggagttcat caccgaaatc aaggtggaga
    15961 tgttcaatca tcagggcgcg ctggcaaacc tgacggcggc aattaacacc acgacttcga
    16021 atattcaaag tttgaatacg gaagagaaag atggtcgcgt ctacagcgcc tttattcgtc
    16081 tgaccgctcg tgaccgtgtg catctggcga atatcatgcg caaaatccgc gtgatgccag
    16141 acgtgattaa agtcacccga aaccgaaatt aatgttttat gaacccaaca cgttatgcac
    16201 gcatctgcga aatgctcgcc aggcggcagc ctgatctgac cgtctgcatg gagcaggtcc
    16261 acaaacctca taacgtttct gcgattattc gtaccgcaga tgccgttggc gtacatgaag
    16321 ttcacgccgt ctggcctgat agccgcatgc gtaccatggc ctctgcggcg gcgggtagca
    16381 acagttgggt gcaggtgaaa acgcaccgca ccattggcga tgccgtcact catctcaaag
    16441 gccagggcat gcagattctg gcaacccatc tttctgataa cgctgtcgat ttccgcgaaa
    16501 ttgattacac tcgcccgacc tgcattttga tgggacagga gaaaacgggc atcacgcagg
    16561 aagcattggc cctggcggat caggacatca tcattccgat gatcggcatg gtgcagtcgc
    16621 tgaatgtttc cgttgcctca gccctcattc tttacgaagc ccagcgtcag cggcaaaatg
    16681 caggcatgta cctgcgtgaa aacagcatgt tgccggaagc agagcaacaa cgcctgttgt
    16741 ttgaaggcgg ctatccggtg ctggcgaaag tcgcaaaacg caaaggcctg ccttatcccc
    16801 acgtcaatca gcaaggcgag atcgaagctg atgccgactg gtgggctact atgcaggctg
    16861 cagggtaagt gccatgacag gtcgcctgtt agatgctgtc ccactcagtt ccctaacggg
    16921 cgttggcgca gcacttagta acaaactggc gaaaatcaac ctgcataccg tgcaggatct
    16981 actcttacac cttcccctgc gctacgaaga tcgcacccat ctctacccca tcggagaact
    17041 actgccgggc gtttatgcca cggtggaagg cgaagtgctg aactgcaata tctccttcgg
    17101 cggtcggcgg atgatgacct gccagatcag cgacggttcc ggcatcctca ccatgcgctt
    17161 tttcaatttc agcgcggcaa tgaaaaatag cctggcgacg ggccgccgtg tactggctta
    17221 tggcgaagca aagcgcggta aatatggtgc ggagatgatc cacccggaat accgcgtgca
    17281 gggcgatctc agcacgccag aattacagga aacgctcacg ccggtttatc caacaacgga
    17341 aggcgtaaag caggccacgc tgcgtaaatt aaccgaccag gcgctggatc tgctcgacac
    17401 ctgcgccatt gaagaactcc tgccgccgga actgtcacaa ggaatgatga cgctaccgga
    17461 agcgttgcgc actttgcacc gcccgccacc gacgctacag cttagcgatc tggaaaccgg
    17521 gcagcatccg gcgcaacgtc gtctgattct ggaagaactg ctggcgcaca acctcagcat
    17581 gttagcctta cgtgccggag cacagcgttt tcatgcccag ccgctgagcg ccaatgatgc
    17641 gctgaaaaat aaactcctcg cagccttacc gttcaagcca acgggcgcac aggcacgcgt
    17701 agtggcggag atcgagcgcg atatggcgct ggatgtgccg atgatgcgcc tggtacaggg
    17761 cgatgtaggt tccggtaaaa cgctggtcgc cgccctcgcc gcgctgcgtg cgattgccca
    17821 cggcaaacag gtggcattga tggcaccaac cgaattactc gccgaacagc acgccaataa
    17881 cttccgcaac tggtttgaac cgctcggtat cgaagtgggc tggctcgccg gtaagcagaa
    17941 aggcaaagcg cgactgtctc agcaggaagc catcgccagc ggtcaggtgc agatgattgt
    18001 tggtacgcac gccatcttcc aggaacaggt gcagtttaac ggtctggcgc tggtgattat
    18061 cgacgaacaa catcgctttg gcgtgcatca gcgtctggcg ttatgggaga aaggccagca
    18121 gcaaggcttc catccgcatc agttgatcat gaccgccacg ccgatccccc gcacgctggc
    18181 aatgacggcg tatgccgatc tcgatacctc ggtgatagat gagctgccgc caggccgcac
    18241 gccagtgact acggtcgcta ttcctgatac ccgccgtacc gacatcattg accgcgtgcg
    18301 ccacgcctgc ataactgaag gtcgtcaggc gtactgggtt tgtacgttga ttgaagagtc
    18361 agagttactg gaagcgcagg cggcggaagc tacctgggaa gagttgaaac tggcgctacc
    18421 agaattgaat gttggcctgg tacacgggcg gatgaaacca gccgagaaac aggcggtgat
    18481 ggcgtcgttt aaacaaggtg agctacatct gctggttgcc actaccgtta ttgaagtcgg
    18541 tgttgatgtg cctaacgcca gcctgatgat tatcgaaaac cctgagcgtc tgggtctggc
    18601 gcagttacac cagcttcgcg ggcgtgtagg tcgtggtgcg gtggcttctc actgcgtgct
    18661 gctctacaaa acgccgctct ccaaaaccgc gcaaattcgg ctgcaagtgc tgcgcgacag
    18721 caacgacggt tttgtgattg cacagaaaga cttagagatt cgcggaccgg gtgaactgtt
    18781 aggtacgcgt cagacgggta atgctgagtt taaagtggcg gatttactgc gcgatcaggc
    18841 gatgatcccg gaagttcagc gcctggcgcg acatattcac gaacgttacc caccacaagc
    18901 aaaagccctg atagaacgct ggatgccgga gacggaacgt tactccaacg catgaccaga
    18961 ctcatcacaa gacaagtcac gcagtaaatc ttcaatctgg atagtgagga tatccattgc
    19021 ctcatacgca tcctgtgcaa ttcgcgccgc cattgcgcct tccggttcac cacgcagaat
    19081 ttcagccgct gcctgtacgc cagccacaat catttcaggt gttaacattt ggcgaagaaa
    19141 agcgcgatta tcagaggacc agccatcttt atggcctaat ggatgccccc agcctatcca
    19201 ttcttgcgac cataacggat tggcttctat gggataaatt agatgcgcgg catactgaat
    19261 agcacacacc gcttttcctg gcgtatccag cgcccaccag cgcgaccaga gagaacgaat
    19321 taacggcgcg gccccaccca gagtattgaa caccgccagc cagcaaagaa cgttattgaa
    19381 accacgctcg ttatccatac gctgcaacgc ggtgtccagt aaaaattgcc gtacctgctg
    19441 gcgttgggaa gcattcatca tctcctgcca gagatagggc cgacacaatg ccttcattaa
    19501 ggattcttcg aagtgatcac cataaccgcc agctaaaacg gtttcatgcc attgcgccag
    19561 gcagagcgga aacagatagc gaaacaactc tatttgcatc ggttcgttat aaacgagatt
    19621 gagtacatag taagcactca aatcccattc gctgatctgc tgccactctt tagccgccag
    19681 cgccttgagt tcggcatctg cgtaatcaaa ttccgtttcc catacacaga gcactggcgg
    19741 ctcatcacca aaaatatggc gacagtgttc acgtagcgtt tccggttcct tccgacgctt
    19801 tttcattttt aaccggcaaa aatcggcaac attaaataca gcttaatcac cagcgcattg
    19861 acgatatcga taaagaacgc accgaccatc ggcaccacca aaaacgccat gtgcgacggg
    19921 ccaaagcgtt cagtgatcgc ctgcatgttg gcgattgccg ttggcgttgc accgaggcca
    19981 aaaccacagt gacccgcagc cagcactgcc gcatcgtagt ttttgcccat catgcgccag
    20041 gtaacgaaga tggcatacaa cgccatgaag atggtctgta ccaccagaat cgccagcatc
    20101 ggcagcgcca gcgaagccag ctcccacagt ttcagcccca tcagcgccat cgccaggaac
    20161 aacgacaggc ttacgttacc cagcacggat accgcacgct caaagacgcg gtaaaagccc
    20221 atcattgaca gaccgttgct cagaatcacg ccaacaaaca gtacacagac gaaggtcggc
    20281 agttcaaaag cagtgccagc caaaagttgc gcaacaattt tccccaccgt caggcagata
    20341 gcaatcagcg cgatagtttc aatcagcacc agcgaggtga tcatgcggcc cacatccggc
    20401 ttttcaaacg ccgtcgggac ttcctggtca tccggaatac cgttcggcgt ggtggagtgt
    20461 ttcaccagat agcgcgccac cggaccgcca atcaagccgc ccagcaccag accgaacgtt
    20521 gcacaggcca tcgccacttc cgtcgcattg gtgaagccat aacgttcaat gaacaattta
    20581 ctccacgcag cgcccgtacc gtgaccgccg gaaagcgtaa tagaaccggc caacagcccc
    20641 atcagcggat caagccctaa caggctagcc ataccaatgc caatggcatt ttgcatcacc
    20701 aacagaccaa caaccacaat caagaagatg ccaaccacac gcccaccggc acgcaaactg
    20761 gcaatgttgg cgttcaggcc aatggtggcg aagaaagcca gcattaacgg atcgcgcagg
    20821 gacatatcaa agttgacttc ccagcccatg ctttttttca gtactagtag cgccagcgcc
    20881 accaacaaac cacccgcaac aggttccggt atggtgtatt tcttcaaaaa ggagacggaa
    20941 tggaccaact tacgcccgag cagcaacgtc agcgttgcgg caacaagcgt tgctaaagta
    21001 tcgagatgaa acatagttac tcctttgtat ccgcatcact ctttcatact cgtcatactt
    21061 catcgcgcag tggcaacttc atgaactatg acgggtatcg agttatgctt tcctgggtca
    21121 aattctcagc tgttaatgaa caaatggcat ggattttaag cagaaaaata ccaaaagtta
    21181 tataaaaaag tgcatttatc tcatttaaat atttagatga ggatgtggca aacgtttgct
    21241 tttctctttc ggtcagctaa aatgcccgct ttgctaccac gggattgttt tcgatgtctg
    21301 tttccaccct cgagtcagaa aatgcgcaac cggttgcgca gactcaaaac agcgaactga
    21361 tttaccgtct tgaagatcgt ccgccgcttc ctcaaaccct gtttgccgcc tgtcagcatc
    21421 tgctggcgat gttcgttgcg gtgatcacgc cagcgctatt aatctgccag gcgctgggtt
    21481 taccggcaca agacacgcaa cacattatta gtatgtcgct gtttgcctcc ggtgtggcat
    21541 cgattattca aattaaggcc tggggtccgg ttggctccgg gctgttgtct attcagggca
    21601 ccagcttcaa ctttgttgcc ccgctgatta tgggcggtac cgcgctgaaa accggtggtg
    21661 ctgatgttcc taccatgatg gcggctttgt tcggcacgtt gatgctggca agttgcaccg
    21721 agatggtgat ctcccgcgtt ctgcatctgg cgcgccgcat tattacgccg ctggtttctg
    21781 gcgttgtggt gatgattatc ggcctgtcgc taattcaggt tgggttaacg tccattggcg
    21841 gcggttacgc agccatgagc gataacacct tcggcgcacc gaaaaatctg ctgctggcag
    21901 gcgtggtctt agccttaatt atcctgctta accgtcaacg taacccttac ttacgcgtgg
    21961 cctcactggt gattgcgatg gcggccggat atgcgctggc gtggtttatg ggcatgttgc
    22021 cagaaagcaa cgaaccgatg acgcaagaac tgattatggt gccaacgccg ctctattacg
    22081 gtcttggcat tgaatggagt ctgctgctgc cgctgatgct ggtctttatg atcacttcgc
    22141 tggaaaccat tggcgatatc acggcgacct ctgacgtttc cgaacagcca gtgtccggtc
    22201 cgctgtacat gaaacgcctg aaaggcggcg tgctggcaaa cggcctgaac tcgtttgttt
    22261 cggcggtgtt taacaccttc ccgaactcct gcttcgggca aaacaacgga gtgatccagt
    22321 tgactggtgt tgccagccgc tatgtcggtt ttgtcgtcgc gctgatgttg atcgtgctgg
    22381 gtctgttccc ggcagtgagc ggttttgtac aacacattcc agaaccggtt ctgggcggcg
    22441 caacgcttgt aatgtttggc accatcgccg cctccggtgt gcgtatcgtt tctcgtgagc
    22501 cgctgaaccg tcgggcgatt ctgattatcg cgctgtcgct ggcggttggt ctgggcgtgt
    22561 ctcagcagcc gctgattttg cagtttgccc ctgaatggct gaaaaacctg ctctcctccg
    22621 ggatcgccgc gggcggtatt actgccatcg tgctgaatct gattttccca ccagaaaaac
    22681 agtaattcac tggcggcggt aagataatta ccgccgcagt ctcacctctt tcaccattcc
    22741 cgccttgagg attgcgcgta aatcgtgcat aactccctta tgtgcatttc acgggatgga
    22801 agaccatgaa atttattggg aagctgcttc tctacattct catcgctctg ttagtggtga
    22861 tcgctggcct ctattttctt ctgcaaaccc gctggggagc agaacatatc agcgcatggg
    22921 tttccgagaa tagcgactat catctggcct tcggggcgat ggatcaccgt ttttccgcgc
    22981 catctcatat cgtgctggag aacgtcacgt ttggtcgtga tggtcagccc gcgaccctgg
    23041 tggcaaaaag tgtcgacatt gcgctaagca gtcggcaact gaccgaacca cgccatgtcg
    23101 ataccatcct gctggaaaac gggacgctga atctcaccga ccagaccgcg ccgctaccgt
    23161 tcaaagccga tcgtctgcaa ctgcgtgata tggcgtttaa tagcccgaat agcgaatgga
    23221 aactgagcgc gcagcgggta aatggcggcg tggttccgtg gtcaccagaa gccggtaaag
    23281 tgctgggtac gaaggcgcag attcagttta gtgccggatc gctttcgctc aatgatgttc
    23341 ctgccaccaa tgtactgatt gaaggcagta ttgataacga tcgcgttacg ctgactaacc
    23401 tgggtgccga catcgcccgc gggacattaa ccggaaacgc gcagcgtaac gccgacggca
    23461 gctggcaagt ggaaaatctg cgcatggcgg atatccgtct acaaagcgaa aaatcgctaa
    23521 ccgacttctt tgcgccatta cgctctgtcc cgtcgttgca gattggtcgc ctggaagtga
    23581 tcgatgctcg tttgcaaggt ccggactggg cggtgaccga cctcgatctc agcttgcgca
    23641 acatgacctt cagtaaagat gactggcaga cacaagaagg caaactgtcg atgaacgcta
    23701 gcgagttcat ttatggttcg ctgcatttat ttgacccgat tataaacgcg gaattttccc
    23761 cgcagggcgt agcgctgcgc cagttcacca gccgctggga agggggtatg gtcagaacgt
    23821 cagggaactg gctgcgtgac gggaaaacgt tgatccttga tgatgcggca attgccgggc
    23881 tggaatatac cttgccgaaa aactggcaac agttgtggat ggaaacgaca cccggttggt
    23941 taaacagcct gcaactgaag agatttagcg ccagccgcaa tctgatcatt gatatcgacc
    24001 ctgacttccc gtggcagctc accacgctcg atggttacgg tgccaacctg acgctggtta
    24061 ccgatcataa atggggcgtc tggagtggct cggcgaatct gaatgccgcc gccgcgacat
    24121 tcaatcgtgt tgatgttcgt cgcccgtcgc tggcgctgac cgccaacagc agcacggtga
    24181 atatcagcga actgagtgca tttactgaaa aaggcattct ggaagccact gccagtgttt
    24241 cacaaacgcc acaacgtcag acccatatca gcctgaatgg acgcggtgtg ccggtgaata
    24301 ttttgcaaca atggggatgg cctgaattac cgttgactgg cgacggcaat attcagctta
    24361 ccgccagtgg cgatattcag gccaatgtcc cgctgaaacc tacggttagc gggcaactcc
    24421 atgccgtgaa cgccgcaaag cagcaagtga ctcaaaccat gaatgcgggc gtcgtttcca
    24481 gtagcgaagt tacatcgaca gagccggtgc agtaaaaatc aatctcgccg ggtacgactc
    24541 agacgtaccc ggcattccat caatagatta caacgtaatt gtcagcgcat tcccttgagg
    24601 cgtcaccacc agcccctgct cactttcagc ctgcgaacca ccttgcagac catttacttt
    24661 cacaatattg cgcaagcaca gtgtccagtt cttcgcctcg cccgcaccag tcacagtaat
    24721 cgtgttgcca gtacgcgccg ctttcagggt gaaaagaacg gaaccgtcag cagcgggcac
    24781 ttcacagatg gcttcatgcc cgtcttgtag attaaagagc tggaatgccg tgccttcgtg
    24841 ccacgcgtaa tcgggacgtt gctcgttgtt gcccagcgcc agtagggtgt tatcacgcac
    24901 ataaacgggc agactcagga agctgtgctg ctgtttatgc cagcgactac catcgagttc
    24961 atcgttgtgc cacagatgtg tccagcgacc ttccggcaag tagaactgca catcgcccgc
    25021 ttcactgaac accggagcaa ccatcacgtt gtcgcctaac atgtattgac ggtcaaggta
    25081 atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg gcgtaccccg
    25141 cgcgttcgca cgagcagctt cacgatacag atacggcatc atgcggcatt tcagttgcgt
    25201 gaagaagcgc accacatcac aggactcatc atcgtaggcc cacggcacac gataagattt
    25261 gctgccatgt aaacggctat ggctggagag caaaccaaac gcgcaccagc gtttgtaaac
    25321 gtgcgccgga gcggtattct caaagccgcc gatatcgtgg ctccagaaac caaaacctga
    25381 aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt tagcgtaaca
    25441 gtcaccaccc cagtgtaccg ggaatttctg cgcaccaacg gaggccgagc gggcaaacaa
    25501 gacggcttct tcctcgccaa cggtgtcctt gagcacgttc cacaccagtt cgttgtagat
    25561 gtacgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact gaacatcgat
    25621 tggaatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga ccagaccttt
    25681 cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa tcgccagacc
    25741 tggctgccat ttatcccact gccataacga accgtccggg cgtttgagta aatagccttt
    25801 ctcttgtaac tctttaaaga caggggattt ttgcccgata taggggttaa tccagacgca
    25861 gatttttagc cctttcgctt tcaggcggcg gatcatccct tccgggtccg ggaaagtcag
    25921 cgggtcccac tcaaaatcgc accactggaa ggctttcatc cagaaacagt caaagtgaaa
    25981 aacatgcagt ggcagattgc gtcccgccat accatcgata aagctgttta ccgtcgcttc
    26041 gtcgtagttg gtggtaaatg aagtggttag ccacaggccg aaggaccacg cgggcggcag
    26101 cgccggacga ccagtaaaac gggtataacg atcaagtacc gctttcggcg tcgggccgtc
    26161 gataacaaag tattcgagat attcactctc aacgctgaac tgcactttgg agactttctc
    26221 cgatcccact tcaaaagaga cgcattgagg atgattgacc agtaccccat agccacggtt
    26281 agtcatatag aacgggatat ttttatacgc ctgttcagta cttgtgccgc cgtcccggtt
    26341 ccaggtctct accgtctggc cattgcgcac cagggcagta aagcgctctc ccagaccgta
    26401 aactgtttcg ccaacgccaa gatccagccg ctcaaacata taatttcgtt gattattcgt
    26461 gtcctgcacg tagccattat ttttcacctg actaccggta atacgttcgc cgttgcgcag
    26521 aaaatccagt gaccagaact cacctttgct gacacgcgcg cttaagttgc cacttttaaa
    26581 ctcagcgtaa cgttctgtgt tttcgattgt gaccttcacg tcctgcaaaa tattgagcgg
    26641 ataatgagga ccgttattca gcgccccctg aaaatgctca atccgtacac cgacaatacc
    26701 ttcctgtggg gagaaaaagc gcagcgtaaa taaaggcgta tcaagctgcc aggttcgttc
    26761 acgcacatca cggggggcag catagaccac catttcatta ccctgctgtt caacctcgaa
    26821 cacctgaagc gggtgaatca aattgaggcc aggttgaatc aaccagtttc cgtcgctaat
    26881 tttcattgcc gttccttagt tctgcacttc ttgagaggtg aattgttgct ggcaacggcg
    26941 tttgccctgg gccagctgtt ccataacggt tttcagattg tgcgtcgtga gtgagtagta
    27001 gcgtttagcg ataatcgcgc tcagcaaata acagatcgcc ggaacaatcg tgaatagcgc
    27061 aataatgatg ctaatcgtgg cgctgttctg cgctttttct gccgcatcat atccgccata
    27121 agccagcatc cagccgataa gagcgccgcc gaaggccaac cccagtttga gcacaaacag
    27181 cgtgccagca aaactgatcc cggtcaggcg tttaccattg caccactcgc cgtagtcgac
    27241 ggtatcggac atcattaccc actggatagg tgtcaccagt tgatgcaaca caccaatcac
    27301 gaagatgaag acaaacatag tgatgctggc ctgcatagga acaaagaaca tcgcgaggct
    27361 aatcactgcc agcagggcgt tcgtccacca gaagatagtg actttacatt tccagtcggt
    27421 cagcggtttt gccagtgcgg aaccaatcag gttacccacg caataagtgg tgagaaaagc
    27481 gacaaacact tccggcgtac ccaaaatcca tgtgacgtaa tacatcatcg ctccaccgcg
    27541 gacgcacacc gccaggatat tgaaaatggt tagtaaaccg acaatccgcc actggtcgtt
    27601 ttgccagata tcacgtaaat cttcccgcat agacgtcgtt gtaggtggtg cttcaacgcg
    27661 ttctttaatg gtgaagaaac agaatgccag catcatgaat gccaccacgg aaaggaccgc
    27721 gataccgccc tggaaaccga gtggtttatt atcaccgcca attaaattaa ccagtggcat
    27781 catcagaaca gtagagagca tgcctcccgc ggtcgccagc acaaaacgcc aggattgcag
    27841 cgagatacgc tgagtcgggt cattggtgat tacaccaccc aatgcgcagt aagggatatt
    27901 gacgacggta taaagtaagg taagtagggt gtaagtaatt gctgcataga tcattttgcc
    27961 gttcatactg agatctggcg tgctataggc cagtacacag acgatcccaa atggcagtgc
    28021 gccacacagt acccacggac gaaatttacc ccagcgagag cgcgttcgat cggccaacaa
    28081 ccccatgcaa ggatcggaaa tcgcatccag tgcacgagcg accaaaaaca tggttccgac
    28141 aaatccggca ggtatgccaa aattatcggt ataaaagaac atcatatata acattacgtt
    28201 atcgaaaata atgtggctgg cggcgtctcc catgccataa ccaattttct ctttaacgga
    28261 caacacttca ctcatcatct gcttttcctt gatgctatag gggtgctgag accgataacg
    28321 c
//
LOCUS       DAZUGK010000040        26965 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZUGK010000040 DAZUGK010000000
VERSION     DAZUGK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.040"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(296..1960)
                     /gene="glnS"
                     /locus_tag="ACHY5W_001533"
     CDS             complement(296..1960)
                     /gene="glnS"
                     /locus_tag="ACHY5W_001533"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFS4446454.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2163..4109)
                     /gene="nagE"
                     /locus_tag="ACHY5W_001534"
     CDS             complement(2163..4109)
                     /gene="nagE"
                     /locus_tag="ACHY5W_001534"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFS4446455.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4442..5242
                     /gene="nagB"
                     /locus_tag="ACHY5W_001535"
     CDS             4442..5242
                     /gene="nagB"
                     /locus_tag="ACHY5W_001535"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4446456.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5302..6450
                     /gene="nagA"
                     /locus_tag="ACHY5W_001536"
     CDS             5302..6450
                     /gene="nagA"
                     /locus_tag="ACHY5W_001536"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4446457.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6459..7679
                     /gene="nagC"
                     /locus_tag="ACHY5W_001537"
                     /gene_synonym="nagR"
     CDS             6459..7679
                     /gene="nagC"
                     /locus_tag="ACHY5W_001537"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFS4446458.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7727..8479
                     /gene="nagD"
                     /locus_tag="ACHY5W_001538"
     CDS             7727..8479
                     /gene="nagD"
                     /locus_tag="ACHY5W_001538"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFS4446459.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8736..10400
                     /gene="asnB"
                     /locus_tag="ACHY5W_001539"
     CDS             8736..10400
                     /gene="asnB"
                     /locus_tag="ACHY5W_001539"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFS4446460.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10779..10855
                     /locus_tag="ACHY5W_001540"
     tRNA            10779..10855
                     /locus_tag="ACHY5W_001540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10813..10815,aa:Met,seq:cat)
     gene            10864..10948
                     /locus_tag="ACHY5W_001541"
     tRNA            10864..10948
                     /locus_tag="ACHY5W_001541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10898..10900,aa:Leu,seq:tag)
     gene            10972..11046
                     /locus_tag="ACHY5W_001542"
     tRNA            10972..11046
                     /locus_tag="ACHY5W_001542"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11004..11006,aa:Gln,seq:ttg)
     gene            11062..11138
                     /locus_tag="ACHY5W_001543"
     tRNA            11062..11138
                     /locus_tag="ACHY5W_001543"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11096..11098,aa:Met,seq:cat)
     gene            11187..11261
                     /locus_tag="ACHY5W_001544"
     tRNA            11187..11261
                     /locus_tag="ACHY5W_001544"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11219..11221,aa:Gln,seq:ctg)
     gene            complement(11415..12590)
                     /gene="ubiF"
                     /locus_tag="ACHY5W_001545"
     CDS             complement(11415..12590)
                     /gene="ubiF"
                     /locus_tag="ACHY5W_001545"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFS4446461.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEITKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12736..14160
                     /gene="miaB"
                     /locus_tag="ACHY5W_001546"
     CDS             12736..14160
                     /gene="miaB"
                     /locus_tag="ACHY5W_001546"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFS4446462.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14313..15353
                     /locus_tag="ACHY5W_001547"
     CDS             14313..15353
                     /locus_tag="ACHY5W_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4446463.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15350..15817
                     /gene="ybeY"
                     /locus_tag="ACHY5W_001548"
     CDS             15350..15817
                     /gene="ybeY"
                     /locus_tag="ACHY5W_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4446464.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15907..16785
                     /gene="corC"
                     /locus_tag="ACHY5W_001549"
     CDS             15907..16785
                     /gene="corC"
                     /locus_tag="ACHY5W_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFS4446465.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16810..18348
                     /gene="lnt"
                     /locus_tag="ACHY5W_001550"
     CDS             16810..18348
                     /gene="lnt"
                     /locus_tag="ACHY5W_001550"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFS4446466.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18745..19653
                     /gene="gltI"
                     /locus_tag="ACHY5W_001551"
     CDS             18745..19653
                     /gene="gltI"
                     /locus_tag="ACHY5W_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFS4446467.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19822..20562
                     /gene="gltJ"
                     /locus_tag="ACHY5W_001552"
     CDS             19822..20562
                     /gene="gltJ"
                     /locus_tag="ACHY5W_001552"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFS4446468.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20562..21236
                     /gene="gltK"
                     /locus_tag="ACHY5W_001553"
     CDS             20562..21236
                     /gene="gltK"
                     /locus_tag="ACHY5W_001553"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFS4446469.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21236..21961
                     /gene="gltL"
                     /locus_tag="ACHY5W_001554"
     CDS             21236..21961
                     /gene="gltL"
                     /locus_tag="ACHY5W_001554"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFS4446470.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22079..23014
                     /gene="rihA"
                     /locus_tag="ACHY5W_001555"
     CDS             22079..23014
                     /gene="rihA"
                     /locus_tag="ACHY5W_001555"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFS4446471.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPDK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23098..24768
                     /gene="hscC"
                     /locus_tag="ACHY5W_001556"
     CDS             23098..24768
                     /gene="hscC"
                     /locus_tag="ACHY5W_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFS4446472.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSLMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAAYRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(24828..26279)
                     /gene="djlC"
                     /locus_tag="ACHY5W_001557"
     CDS             complement(24828..26279)
                     /gene="djlC"
                     /locus_tag="ACHY5W_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFS4446473.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSVGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYITAFLAMAGPWLIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQTEEPESEDARYYLCAQRLYCGERESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26276..>26965)
                     /locus_tag="ACHY5W_001558"
     CDS             complement(26276..>26965)
                     /locus_tag="ACHY5W_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4446474.1"
                     /translation="YLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNR
                     DDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMC
                     CGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMHYFSAYS
                     LGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPE
                     CPVTLKDVSDL"
BASE COUNT         6623 a         6986 c         6869 g         6487 t
ORIGIN      
        1 ttatgttttg ttattttaaa tttattttat ggataaattg cgaagcggaa ttaattcgcg
       61 aagaaaagca gaaaaaacgc cgctgaacgc ggcgttttgg attgtttgca tatttcttgc
      121 cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaactcaa tatattgcag
      181 agatcatgta ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga aactgtttgt
      241 ctgttccttg ccggatgcga cgtaaacgcc ccatccggca tagcgaaact taaaattact
      301 cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg
      361 ccgtagaatg gcggctatcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac
      421 ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg
      481 ggttaatcac cgacaggaaa tcatccgcag cacctgggtt cggcacgctg aacagacggt
      541 catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt
      601 tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa gtacagaaga
      661 tggtggtgat attaccttcg gcatctttct cgacgcgttc tgccttaatc acataagcat
      721 tacgcagacg cacttcttta ccgagcacca gacgtttgta ctgcttgtta gcttcttcgc
      781 ggaaatcggc gcgatcgatc caaatctcac cgctaaacgg cacctgacgg ctgcccattt
      841 ccggtttgtt cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga
      901 taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt
      961 cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga
     1021 ttcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc agaccggaaa
     1081 tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaggttca
     1141 gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg
     1201 ggtgaacagg aatggtgatg ttgtccagca cccagtcata cagacgacgg ttgtcctgga
     1261 actcaagcgt acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga
     1321 agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa
     1381 tgcggtacag caccggatcg cgcattacga taaacggtga agccatgtcg attttcgcac
     1441 gcaggcaggc tttaccttct tcaaaaccgc cggtacgcat tttctcgaac agcgccaggt
     1501 tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgtgtc agggtgccac
     1561 ggtattcgcg gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca
     1621 gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag
     1681 accagtgaaa acctaaccac tcaacgtcgt ttttgatcga atcgacatac tcgatatctt
     1741 cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga
     1801 tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg
     1861 gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct
     1921 gacgaataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta
     1981 aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagtttttt
     2041 aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag cggcagagaa
     2101 gatctccacc gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag gcataaagca
     2161 gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta
     2221 atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgaacgg
     2281 gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc
     2341 gcgccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt gtcgataccc
     2401 atgtggacga caatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg
     2461 aagattttca cgattgttcc tgcggctggt gatacgacga ttttatctgt cggtttgacc
     2521 gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc
     2581 acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc
     2641 tgcggttttg ctacaggagc ggcagttgcc ggagttgctt cagctgacgc agctgctacc
     2701 ggaccgcggg caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca
     2761 atcacctgaa tagtctgttt gttcagtttt actacaccag aagcccccag acgtttacac
     2821 atcgtatcgt taacgcgggc agagtcagcc actgtaaggc gcagacgggt gatacaggcg
     2881 tcaattgctt tcaggttgtc agtgccgcca accgcagcaa tatagttggt tgccagttga
     2941 gtcagacctt cttcagtatt gctgttagcc tcttcagtaa cgatctcgtc ttctttatct
     3001 tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag
     3061 atagcgaaga agacaacacc catcaccagc agcatccaga cgttctggct ggcggccggc
     3121 aggttataca tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc ggcatggata
     3181 cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc
     3241 agcggagcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca
     3301 gtaacagcaa cagaaagcag catcccacca accatcggac gacgctcttt cggtgctgcg
     3361 aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa gaagccggac
     3421 atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc
     3481 gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga
     3541 tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga acccagcgcg
     3601 cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg cggccagaca
     3661 taaccaaaaa tggccgccag caccaggcag aagaagccgg tggcaatcgg cacaaagcgt
     3721 ttgccgccga agaaactcag gaagtccggc agtttaatat cggaccaacg gttataggct
     3781 gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg
     3841 atggtcacca tcgctttggt taacacaaag taacctaccg cacccgccag cgccgccgca
     3901 cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta
     3961 tcaaaaatcg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg
     4021 aatcgcagca acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg
     4081 agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct
     4141 cgtttaaagc cgtattttta ttttgctgca aattgtactg ccgatgttct gtaatcagat
     4201 tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt
     4261 gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaacc
     4321 agaaaactta ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc
     4381 aggttacgct taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat
     4441 aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat
     4501 cgtcaatcgt atcaatgcgt tcaaaccgac tgccgatcgt ccgtttgtac tgggcctgcc
     4561 gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata aagcaggcca
     4621 ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga
     4681 gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc
     4741 agcagaaaac atcaaccttc tcaacggcaa cgccccggat atcgacgccg agtgccgcca
     4801 gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa
     4861 cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa
     4921 aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca
     4981 ggtgccaaaa tatgccctga ctgtcggtgt tggtacattg ctggatgccg aagaagtgat
     5041 gattctggtg ctgggtagcc agaaagcact ggcactgcag gccgccgttg aaggttgcgt
     5101 aaaccacatg tggaccatca gctgtctgca actgcatcca aaagcgatca tggtgtgcga
     5161 tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc
     5221 agaaaatatc aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat
     5281 tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac
     5341 gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg
     5401 gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc
     5461 ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa
     5521 gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact
     5581 aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt
     5641 atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggtccg
     5701 tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc
     5761 gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg
     5821 gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc
     5881 ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg gattaccttt
     5941 gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc
     6001 gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat
     6061 tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac
     6121 gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac
     6181 taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg
     6241 attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt
     6301 atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc
     6361 gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc
     6421 gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc
     6481 agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg
     6541 accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca
     6601 gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc
     6661 aggcctccac cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc aatttccacg
     6721 caatcggcgt acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca
     6781 aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg
     6841 ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga
     6901 tcgccatttc cgtgatcctg ccagggcttg ttgacccgga cagcggcaaa attcattaca
     6961 tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagag cgttttaaag
     7021 tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg
     7081 caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga
     7141 ttatctctaa cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata
     7201 ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta
     7261 tcgctgccaa cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga
     7321 gccgcgtgcc gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg
     7381 atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca
     7441 ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg
     7501 ataaagtgct gctccctgct attgaaagct gcattaatac ccaggcgctg aaggcgtttc
     7561 gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg
     7621 cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat
     7681 gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa
     7741 tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc
     7801 ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc
     7861 ttcgcagacc gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtacctga
     7921 cagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa
     7981 gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac
     8041 tattaccgat gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga
     8101 catgatgcat aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc
     8161 ggatactcac gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa
     8221 aatctccggg cgcaaaccgt tctatgttgg taagccgagt ccgtggatta tccgcgcggc
     8281 attaaacaaa atgcaggcac attcggaaga aacggtgatt gtcggcgata acctgcgtac
     8341 cgatattctg gccggcttcc aggcaggtct ggagacgatt ctggtgcttt ctggtgtttc
     8401 gtcgctcgac gatatcgaca gtatgccttt ccgccccagc tggatttacc cgtcagtcgc
     8461 tgaaatcgac gttatctgaa tagcaaccac gtctcaggac gtggtttctt atttttaccc
     8521 atcattaaaa tcatcccatt caataaaacc cgccaaaaat tatcgatcca ttaataaata
     8581 catcaccgca ccctcccctt tttcacaaat cagcaatcac cattacgttt tttatttttt
     8641 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
     8701 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
     8761 atatcaaaac agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc
     8821 gtcatcgtgg cccggactgg tccggtattt atgccagtga taacgccatt ctcgcccacg
     8881 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
     8941 cccacgtact ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc gctgaatacg
     9001 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
     9061 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
     9121 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
     9181 gctatgacga acatggtcag ctgtatgtgg cctcagaaat gaaagccctc gtgccggtat
     9241 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagtcaggac ggcgaaatcc
     9301 gttcttatta tcatcgcgac tggttcgact acgatgcggt gaaagataat gtcaccgaca
     9361 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
     9421 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
     9481 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac
     9541 actcctttgc tgtaggtctg ccgggttcac cggatctgaa agcagcccag gaagtggcaa
     9601 accatctggg cactgtgcat catgaaattc acttcactgt acaggaaggt ctggacgcca
     9661 tccgcgacgt gatttaccac atcgaaacct atgatgtgac cactattcgc gcttcaacac
     9721 cgatgtattt aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg ctgtccggtg
     9781 aaggttctga tgaagtgttt ggcggttatc tttacttcca caaagcaccg aatgccaaag
     9841 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcccgtg
     9901 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaagaaat
     9961 tcctcgacgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaagatgg
    10021 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
    10081 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
    10141 cggcgcagca ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca
    10201 caccgacctc aaaagaagcg tatctgtacc gggagatctt tgaagaacta ttcccgcttc
    10261 cgagcgccgc tgagtgcgtg ccgggcggtc cttccgtcgc ttgttcttcc gctaaagcga
    10321 tcgaatggga tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg gttggtgttc
    10381 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
    10441 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
    10501 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccggcaccat
    10561 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg ggaaaaaaca
    10621 caaagtaaac aataattgac gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc
    10681 actttcgagc aattttcctt gaaaaagagg ttgacgctgc aaggctctat acgcataatg
    10741 cgccccgcaa cgccgataag gtatcgcgaa aaaaagatgg ctacgtagct cagttggtta
    10801 gagcacatca ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccatcttt
    10861 tttgcgggag tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt
    10921 gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc
    10981 gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc
    11041 cagccatcga agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa
    11101 tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatatg
    11161 ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct
    11221 gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa agctcgcttc
    11281 ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga tacgtcttat
    11341 ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg cacgcatccg
    11401 gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt caacacgcca
    11461 gcacgctccg ccgccatcaa accaagatta cgcacaaaac gcagcggcgg cagattatta
    11521 ctgaatccgg cataaaacag atccataccg ctctgcataa tgaagttatc cgccatgcgc
    11581 cgcatctggt aacgtttgag gacaggataa ctggcccacg cttcgccgta gctgcgggca
    11641 ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac cccctgcccc
    11701 gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccctgg ctgcacatac
    11761 tgcaacgcat ggcgacgcgt cagcggaaac gcaccagcgg caagcggtgt aacgtaaccc
    11821 agacgcgacg ggaaatgctt cgtgatttcc gcctggagct gtgccatatt catattctgc
    11881 aactggcgaa tacgcgccgg agagtcatac cacaccagcg atgcccagtt atcaaacaac
    11941 ggcagaaacg cacgcggtcc gtccggagta aattgctgcc aggtgctatc gccgggatcg
    12001 ttctcgcact ggacgctaat caacatgcac gactgcgcat actgccatgc atgaacgcca
    12061 attcccgcca tctgccgcac ctgcgaattt gcgccgtcgg caccaatcac cagcttcgag
    12121 cgaatcactt caccgccttt cagctccagc tcctgaagat cattatctcg atgtaatgca
    12181 atcagcgagc ctggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaac
    12241 gcctgttgca ggacagtgtt ttccaccata tagccaagca gcggtagctt aagttcagcg
    12301 gcgtcaaaca ccacatgcgc cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg
    12361 caacgcatgg cctgtactgc atcccagacc cctaaccctt taagcaatga taccgaagcc
    12421 gcgctgatcg ccgagatccg cacgtccggt tggctatcag cgacaaacgg cgctggttct
    12481 gcgtgctcga tcaccgttac cgcaaatccg tgctgtgcca gccccagcgc cagtgcgccg
    12541 ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat
    12601 cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca
    12661 tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt
    12721 cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac
    12781 gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta tcaactgacc
    12841 gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct gctcaatccg cgagaaggct
    12901 caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac
    12961 ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc
    13021 gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc ggagatgatc
    13081 aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag
    13141 tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa
    13201 ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga agaggtaagc
    13261 cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg cgtgcgtgaa
    13321 gtcaacctgc tcggtcagaa cgtgaacgcc tggcgtggtg aaaactacga cggcaccaca
    13381 ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga tcgtattcgc
    13441 tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta ccgcgacacg
    13501 ccggagctgg tgagcttcct gcacctgccg gtacagagtg gttccgatcg cattctgaac
    13561 ctgatgggcc gtacccatac ggcgctggag tacaaagcga tcatccgtaa actgcgtgcg
    13621 gcgcgcccgg atattcagat cagttctgac ttcatcgtcg gcttccctgg cgaaaccacc
    13681 gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc
    13741 tttatcttct ctgcacgtcc aggaacgcct gccgctgata tggttgatga tgttccggaa
    13801 gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca agcgatggcg
    13861 tggagccgcc ggatgctcgg caccacccag cgtattctgg tagaaggtac atcgcgtaag
    13921 agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt cgagggcaca
    13981 ccggatatga ttggtaaatt cgtcgatgta gaaattaccg acgtctaccc gaactctctg
    14041 cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc gcgtggcaga aacaccggaa
    14101 tcggtgatag cccgtactcg caaagaaaac gaccttggcg tgggttatta tcagccgtaa
    14161 ttctcaggcc cgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg
    14221 caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca
    14281 gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc
    14341 tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca
    14401 agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac tttaaactga
    14461 ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata
    14521 ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta
    14581 aagaagcacg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc aaagcggtca
    14641 atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg
    14701 ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct
    14761 acctggcagt ggctgcagca gttgatgccc tggagcgtca ggaaattcgc cgtattctgc
    14821 tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc gatttaagcc
    14881 agaaagtaga cccgtatctg cgcccgctgt acgacgcgct gtttgaaatg ctgggctttg
    14941 agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcaccgctg gcctatatgc
    15001 gtggtcgtac gctgaacgac gcctttatca ttctcgatga gagccagaac actaccatcg
    15061 aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggtg
    15121 acgtcacgca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat gccatcgaag
    15181 tgctggctga tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgcc
    15241 acccggtggt ggcgcgtatc gttaacgcct atgaagcctg ggaagaagca gaacaaaaac
    15301 gaaaagcggc gctggcggca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt
    15361 gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag agagccagtt
    15421 tcagacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag tgacgattcg
    15481 cgtggtcgat accgccgaaa gccacagtct caatctgacc tatcgcggta aggataagcc
    15541 gaccaacgtg ctctccttcc cgtttgaagt gccgcccggc atggagatgt cgctactggg
    15601 cgatctggtt atctgccgtc aggtggttga gaaggaagca caggagcaag gcaaaccact
    15661 ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca
    15721 catcgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta tgcttgctct
    15781 gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt tgactggcag
    15841 acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac
    15901 gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt
    15961 ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg
    16021 gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc
    16081 gaaggggtga tggacatcgc agaccaacgc gtccgcgaca tcatgatccc ccgctcccag
    16141 atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc
    16201 gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg
    16261 gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg
    16321 ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt
    16381 cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt ttccggtctg
    16441 gtaaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga atatgacgaa
    16501 gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct
    16561 tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga agtcgacact
    16621 atcggtggtc tggtgatgca ggcatttggg catcttccgg cacgtggcga aaccatcgac
    16681 atcgacggtt accagttcaa agtggcgatg gccgacagtc ggcgtattat tcaggttcat
    16741 gtcaaaatcc cggatgactc accccagccg aagctggatg aataaaaccg aaactggata
    16801 gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg
    16861 ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg gcctgcggcg
    16921 attatttcgc tgatggggct tcaggcgttg acctttaacc gccgtccact ccagtctgcc
    16981 gctattggct tttgctgggg atttggtctc tttggcagcg gtattaactg ggtctatgtc
    17041 agcattgcga cctttggcgg aatgcctggc ccggttaaca tcttcctggt ggtactgctg
    17101 gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcacg cctgtggccg
    17161 aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt gaccgagttt
    17221 ctgcgcggtt gggtactgac aggcttcccg tggttacagt tcggctatag ccagattgac
    17281 ggcccgttaa aagggctggc acctataatg ggcgtggaag ccattaactt cctgctgatg
    17341 atggttagtg gcctgctggc cctggcgttg gtcaaacgca actggcgtcc gctggtagtg
    17401 gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt taccccgcaa
    17461 ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc gctgaaatgg
    17521 gacgaaggcc agcttcttaa tacgctgaag atttactaca acgcaacggc accgctgatg
    17581 ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga aattaatcag
    17641 caaccgttcc tcaaagcact ggacggtgag ttgcgcgata aagggagtac gctggtaacc
    17701 gggattgtcg acgcgcgcct caataagcag aaccgctacg atacctacaa caccattatc
    17761 acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgttataa caaaaaccat
    17821 ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt agcaccgttc
    17881 tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc gctgtcggca
    17941 aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga gcaagtgcgc
    18001 gataacttcc gcccggatac cgactatctg ctgactatct ccaacgatgc gtggtttggt
    18061 aagtctattg gtccatggca gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg
    18121 cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag
    18181 attcaggcga tgatcccgca gttcacccgc gaggtgttaa ccactaacgt gacgccgacc
    18241 accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct gacggcactg
    18301 tttggttttg ccgctgtgct gatgagtctg cgtcaacgac gtaaataatc ccttcttatc
    18361 gtgccggaat acatcccgta tttcggcacg ctcaccggat tcggcatcag tgcgttatcc
    18421 ggataacaaa attcccctat ccaactttgg cacatctatt gctttgttat acaaggcaaa
    18481 gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt agcaccaaaa
    18541 gagtgcattt taagaatttt gcttcggaat ggtgcggcgc acctcccaaa tataaacaat
    18601 aaaacagcaa tatctttaca tttagccaaa ctaaatgtta cctaacaagc acaacactgc
    18661 acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca tgcgttctta
    18721 acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc aatcctcgcc
    18781 ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc aggcagtacg
    18841 ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc ttcagtacct
    18901 ttctcttatt acgacaatca gcaaaaagtg gtgggttatt cgcaggatta ctccaacgcc
    18961 attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtgaa actgattccg
    19021 atcacctcac aaaaccgtat tcctcttctg caaaacggca ctttcgattt tgaatgtggt
    19081 tctaccacca acaacgtcga acgccaaaaa caggcggctt tctctgacac tattttcgtg
    19141 gtcggtacgc gcctgttgac caaaaagggc ggcgatatca aagattttgc cgacctgaaa
    19201 ggcaaagccg tagtcgtcac ttccggcact acctctgaag ttttgctcaa caaactgaat
    19261 gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag atcacggtga ctctttccgc
    19321 accctggaaa gcggtcgtgc cgttgccttt atgatggatg atgccctgct ggccggtgaa
    19381 cgtgcgaaag cgaagaaacc agacaactgg gatatcgtcg gcaagccgca gtctcaggaa
    19441 gcctacggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat ggatgacact
    19501 atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataaatg gttcaaaaat
    19561 cccattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat gaaagcactg
    19621 ttcaaagaac cgaatgacaa agcactgaac taattacaag aaccaggggc ggaaattcta
    19681 gccctctcga ttgttacgta gcacggacag actatacgcc tgatggtcgt tccccatcgg
    19741 gcctgaaaac gcaatacgct gggtaacaat cttcgagggt agcagttaac gctgctaccc
    19801 tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt tttacaacaa
    19861 gccccgttcg gcaacaccac ctatcttggt tggatctgga gtggttttca ggtgacgatc
    19921 gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt cggcatttta
    19981 cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga actgttccgc
    20041 aacgtgccgt tgattgtgca gttctttacc tggtatctgg tgatcccgga gctgctgccg
    20101 gaaaaaatcg gcatgtggtt taaggcggaa ctggacccaa atattcagtt tttcctctcc
    20161 tccatgctct gcctggggct gtttactgcg gctcgcgttt gcgaacaggt acgcgccgcc
    20221 attcagtcgc tgccgcgcgg gcaaaaaaac gccgcgctgg cgatggggct gacgctgccg
    20281 caggcttacc gctacgtgct gctgcctaac gcgtatcgcg ttatcgtccc accgatgact
    20341 tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgactatcgg tctggtggat
    20401 atggcggcgc aggcgggtaa attgctcgat tactcggccc acgcctggga gtcgtttacc
    20461 gccattacgc tggcgtatgt gttgattaac gcttttatca tgctggtgat gacgctggtt
    20521 gaacgtaaag ttcgcctgcc tggcaacatg gggggcaaat aatgtacgag tttgactgga
    20581 gttccattgt cccttccctg ccatatctgc tcgacgggct ggtgatcacg ctgaaaatca
    20641 ccgtcacggc agtcgtgatt ggtattttgt ggggcaccat gctggcggtg atgcgcttgt
    20701 ccagctttgc gcccgtagcg tggtttgcca aagcctacgt taacgtattc cgctcgattc
    20761 ctttagttat ggttttgctg tggttttacc tgatcgtgcc gggttttctg caaaatgtgc
    20821 tgggattatc gccaaaaaat gatatccgtt taatttcggc gatggtggcg ttttcgatgt
    20881 ttgaagcggc ctactattca gagattatcc gcgccggtat tcaaagtatt tctcgtgggc
    20941 agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa ctgattattc
    21001 tgccgcaggc tttccgcgcg atggtgccgc tgttgctcac tcagggcatc gtactgtttc
    21061 aggatacctc actggtgtat gtgttatcgc tggccgattt cttccgtacc gcctcaacca
    21121 ttggtgagcg tgatggtacg caggttgaga tgatcctgtt tgccggattt gtttatttcg
    21181 tgattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca gcataatgat
    21241 taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg actgctcaac
    21301 cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg gaaaatcaac
    21361 gctgattaaa accgtcaacg gtcttgaacc ggtgcagcaa ggtgaaatca ccgttgatgg
    21421 catcgtggtt aacgataaga aaaccgatct ggcaaagcta cgttcccgcg tcggcatggt
    21481 gttccagcat tttgaactgt tcccccatct gtcgattatc gaaaacctga ccctggcgca
    21541 ggtgaaagtg cttaaacgcg ataaagcgcc tgcgcgtgaa aaggccctga aactactgga
    21601 gcgcgtgggg ctttccgcac atgccaataa gtttccggcg cagctttccg gcggtcagca
    21661 gcagcgtgtg gcaatcgctc gcgcgctgtg tatggatcct attgccatgc ttttcgacga
    21721 acccacatcg gcgctggacc cggagatgat caacgaagtg ctggacgtga tggtcgaact
    21781 ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg cccgtaaagt
    21841 ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc cgaaagacgc
    21901 tttcttcgat gatccgaaat cggaccgtgc aaaagacttc ctcgcgaaaa tcctgcacta
    21961 atccttctgg cgcgcatacc caggtgcgcg ccatctcacg ctttatcgtt tcggccctcg
    22021 tatctgttac attccggcgt tacccttgtc gcaaagaaac acacaacaag gagcaacaat
    22081 ggcattgcca attctgttag attgcgaccc aggtcatgat gacgctatcg caatagttct
    22141 cgccctcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg gaaatcagac
    22201 acctgataaa acattgcgta atgttttgcg tatgctgaca ttgcttaatc gcaccgatat
    22261 tccggtggca ggcggcgcag taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt
    22321 gcacggcgaa agcggtctgg atggcccggc attaccagaa ccgacgtttg caccgcaaaa
    22381 ctgtacggcg gtggagctga tggcgaaaac gctgcgtgaa agtgcggaac ctgtcacgat
    22441 tgtgtctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc cggaactgca
    22501 tagcaaaatt gcccgtatcg tgattatggg tggcgctatg gggctgggta actggacgcc
    22561 tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct tccagtcagg
    22621 gatcccggtg gtgatggccg gtctggatgt tactcacaaa gcacaaatcc acgttgaaga
    22681 cactgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg aactgctgga
    22741 tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac cgctgcatga
    22801 cccatgcacc atcgcctggc tgttgaaacc ggagttgttt accactgttg agcgctgggt
    22861 tggcgtggaa acacaaggga aatataccca ggggatgacg gttgttgatt attattatct
    22921 gactggcaat aaaccgaatg ccactgtaat ggtcgatgtt gatcgtcagg gctttgttga
    22981 tttactggct gatcgtctga aattttacgc ttaacacacg tcattgatta agatcaccac
    23041 gcgttacctg ggtggtctta atctgtttat ctctttttct ttcaagatat aaatgcaatg
    23101 gataatgcag aactcgccat tggtatcgat ctcggtacta ccaatagttt aattgccgtc
    23161 tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt aacaccatcc
    23221 ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt atcacggcgt
    23281 acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag taataccaac
    23341 tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt attacgctca
    23401 ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt gatctctgtt
    23461 ccggcttatt tcagcgatga acaacgcaag catacccgtt tagcagcgga gttagccggt
    23521 ttaaatgcag ttcgcctgat taatgagcca acggcagctg cgatggcgta tggcctacat
    23581 acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt tgacgttacg
    23641 gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga caacttcctc
    23701 ggtggcgaag attttaccca tatgctggtc gatgaggttt taaaacgcgc ggatgtcgcc
    23761 aggaccacgc ttaacgagag tgaactggca gccttgtatg cctgtgtgga agcggcaaaa
    23821 tgtagcaatc aatcgctaat gcacattcgc tggcagtacc aggatgaaac gcgggaatgc
    23881 gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg
    23941 ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga cagtctggtg
    24001 ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc
    24061 aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc agcaatccag
    24121 gcagcctacc gcttacgcag tgaagatatt gaagaagtga tcctcactga tatttgccct
    24181 tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc gccgattatt
    24241 gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa
    24301 caggattcta ttacggttaa cgtctatcag ggagaaaacc acaaagttaa aaacaacatt
    24361 ctggtggaat ccttcgatgt gccgttgaag agaaccgggg cttatcagtc gattgatatt
    24421 cgctttagtt atgatatcaa cggtttgctt gaagttgacg tgcttctgga agacggcagc
    24481 gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca gattgaagag
    24541 agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat caatcgtacc
    24601 tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt
    24661 ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat
    24721 gaggtcaggc ggcgggcgag cgtttattta gccattgaga tcccataatc acggctgccg
    24781 gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac
    24841 atcgcgattt tgccgagagc ataacctatc accagcaccg ttaagctgat tttgataatg
    24901 ctggtaatgc tctgtggttt ttcatcctca tcattttttt cttcatggtt ttcaggtgtt
    24961 ggctcatcga catcttcatc ctccggctcg agtttcactt tcgtcaacag catccgggtg
    25021 ataatggcct ctaatggatg agcatctcca ccttccagcc ttgtattaag acgggccagc
    25081 atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata caaatccagt
    25141 aacggtgaat cttgctctgt ctcgctaccg actctcgatt tccaccacgc ccatgctttg
    25201 cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgatagttt tccgctatgt
    25261 aaaatttccg cccataacag acgcgtccgg gcgctctcac caggaacata tttcaacggt
    25321 ttaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa cgcgaaataa
    25381 tccgggcaat gttgtgcgct ccactgcaac aacaaattat cggcttgtgt agatgggtaa
    25441 cttccccagt acgcacagag atcggcaagc aggctttccc tctcgccgca gtacaaacgt
    25501 tgtgcacaca agtaatatcg ggcatcttcg ctttctggtt cttccgtttg ccactgttgg
    25561 gcaacaggaa tgagttccgc catacctgtt tgcaccgagc tgtaccagcg caacagtttg
    25621 cgatgcaact tcgcatcatc aggaattaac cacggacctg ccatcgccaa aaacgcagtg
    25681 atatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatactc aacagtctgg
    25741 ttctgcaacg caatgggata ttccagaagg ctaaggaaat cgaaacaatc accagcctga
    25801 atggcctcaa gaaaagcctc cctttcttcc tcatccattc ctacgctgtc attaaacgaa
    25861 tgccagttca aacgctctgc cagcaaagaa gcacaattta atgaaagata tcgcgcttct
    25921 atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt ccaggtattt
    25981 aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc tgaatccagt
    26041 aatgtcctga atgcacgcag tatttcatgt tcagcggcgg catctttatc ttcattatct
    26101 gcttccccga caggatttac cgccagtcgc agcgcctctt cataggcctg gcgtaatgct
    26161 ttaaaccctt gcggatcggt ttcgggatga cataacggta agcgagcaag ataagcctgg
    26221 cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca tgttttcata
    26281 ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat ggttgccagg
    26341 gaagcgtggc atagaaacga tcgcttcccg ctgccagctc ttcgaacata cgactcccgg
    26401 cgctatcgta tttgtacagg gttagcgcct cgcgtagcga tgtgttgtct tcgcactgag
    26461 aagattgcca gtagagacgt cctaacgaat aggcagaaaa atagtgcatc cagctgtgat
    26521 agtaatgatg agctcgtaca tagacgcgcg attgcagcca taaactctcg tcttccgtca
    26581 gccacttgtt gagcacaccg acccgtgaca gaaaccccat tcgaacataa tcccaggctt
    26641 tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta taaatcaacc
    26701 ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag cgatgccatg
    26761 tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga taaatagtct
    26821 gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat tcttttatcc
    26881 cagtatacat ttttggcgag tcatgcgcgg gtttgcaacc ctgattaagt atttccatgg
    26941 gcgcagaaag cgcaaaaaga agata
//
LOCUS       DAZUGK010000041        26793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZUGK010000041 DAZUGK010000000
VERSION     DAZUGK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.041"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1647
                     /gene="rtcR"
                     /locus_tag="ACHY5W_001559"
     CDS             49..1647
                     /gene="rtcR"
                     /locus_tag="ACHY5W_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFS4446475.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFGGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHAALTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1629..2387)
                     /locus_tag="ACHY5W_001560"
     CDS             complement(1629..2387)
                     /locus_tag="ACHY5W_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFS4446476.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2404..3234)
                     /gene="glpG"
                     /locus_tag="ACHY5W_001561"
     CDS             complement(2404..3234)
                     /gene="glpG"
                     /locus_tag="ACHY5W_001561"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFS4446477.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3279..3605)
                     /gene="glpE"
                     /locus_tag="ACHY5W_001562"
     CDS             complement(3279..3605)
                     /gene="glpE"
                     /locus_tag="ACHY5W_001562"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFS4446478.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3795..5300
                     /gene="glpD"
                     /locus_tag="ACHY5W_001563"
     CDS             3795..5300
                     /gene="glpD"
                     /locus_tag="ACHY5W_001563"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFS4446479.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5514..6113)
                     /locus_tag="ACHY5W_001564"
     CDS             complement(5514..6113)
                     /locus_tag="ACHY5W_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446480.1"
                     /translation="MNKIRLYISPLILGFVIVPLLVWPTVIALAIFISTLTFMGELLF
                     SISPYGARISLLELQLGLLAEYTLLFCAICCVGWQFARITQSELKSKLHYWLVFVPVY
                     FWLLLWNIIFYVAPAKIALLENMRSFFLTVVWLPLNFSPFWSQPWIDFVGPISAQLGF
                     ALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLSMMAWALR"
     gene            complement(6116..6883)
                     /locus_tag="ACHY5W_001565"
     CDS             complement(6116..6883)
                     /locus_tag="ACHY5W_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446481.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNILVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAVNS
                     IPGFWSAASLACLLNQYREGYIRDNQDDIDNAITAQINTWADNNQSILKERARQAIEN
                     PLKQSEQGTQYQVWEDKWMIDECLCVMYDRSSESPGQ"
     gene            complement(6886..7596)
                     /locus_tag="ACHY5W_001566"
     CDS             complement(6886..7596)
                     /locus_tag="ACHY5W_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446482.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQV
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQLARG"
     gene            complement(7612..9120)
                     /locus_tag="ACHY5W_001567"
     CDS             complement(7612..9120)
                     /locus_tag="ACHY5W_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4446483.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTIYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPALLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDIWAW
                     QPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9245..11692)
                     /gene="glgP"
                     /locus_tag="ACHY5W_001568"
     CDS             complement(9245..11692)
                     /gene="glgP"
                     /locus_tag="ACHY5W_001568"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFS4446484.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11711..13144)
                     /gene="glgA"
                     /locus_tag="ACHY5W_001569"
     CDS             complement(11711..13144)
                     /gene="glgA"
                     /locus_tag="ACHY5W_001569"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFS4446485.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13144..14439)
                     /gene="glgC"
                     /locus_tag="ACHY5W_001570"
     CDS             complement(13144..14439)
                     /gene="glgC"
                     /locus_tag="ACHY5W_001570"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFS4446486.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14457..16430)
                     /gene="glgX"
                     /locus_tag="ACHY5W_001571"
     CDS             complement(14457..16430)
                     /gene="glgX"
                     /locus_tag="ACHY5W_001571"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFS4446487.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16427..18613)
                     /gene="glgB"
                     /locus_tag="ACHY5W_001572"
     CDS             complement(16427..18613)
                     /gene="glgB"
                     /locus_tag="ACHY5W_001572"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFS4446488.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18886..19989)
                     /gene="asd"
                     /locus_tag="ACHY5W_001573"
     CDS             complement(18886..19989)
                     /gene="asd"
                     /locus_tag="ACHY5W_001573"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4446489.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20181..20774
                     /gene="yhgN"
                     /locus_tag="ACHY5W_001574"
     CDS             20181..20774
                     /gene="yhgN"
                     /locus_tag="ACHY5W_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFS4446490.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20831..22171)
                     /gene="gntU"
                     /locus_tag="ACHY5W_001575"
     CDS             complement(20831..22171)
                     /gene="gntU"
                     /locus_tag="ACHY5W_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS4446491.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     VIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22175..22702)
                     /gene="gntK"
                     /locus_tag="ACHY5W_001576"
     CDS             complement(22175..22702)
                     /gene="gntK"
                     /locus_tag="ACHY5W_001576"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4446492.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22841..23836)
                     /gene="gntR"
                     /locus_tag="ACHY5W_001577"
     CDS             complement(22841..23836)
                     /gene="gntR"
                     /locus_tag="ACHY5W_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFS4446493.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(24060..24755)
                     /gene="yhhW"
                     /locus_tag="ACHY5W_001578"
     CDS             complement(24060..24755)
                     /gene="yhhW"
                     /locus_tag="ACHY5W_001578"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFS4446494.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24878..25915)
                     /gene="yhhX"
                     /locus_tag="ACHY5W_001579"
     CDS             complement(24878..25915)
                     /gene="yhhX"
                     /locus_tag="ACHY5W_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4446495.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26248..26736
                     /gene="yhhY"
                     /locus_tag="ACHY5W_001580"
     CDS             26248..26736
                     /gene="yhhY"
                     /locus_tag="ACHY5W_001580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS4446496.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6694 a         7170 c         6667 g         6262 t
ORIGIN      
        1 agccgattta ccgataatga aatatcgtct tttataagga tatctaagat gcgtaaaaca
       61 gtggcttttg gctttgtcgg aacggtgctg gattatgccg ggcgcggcag tcagcgctgg
      121 tcgaaatggc gtccgacgct ttgtttatgc cagcaagaat cgttggtcat cgatcgactg
      181 gaattgttgc atgacgcccg ctcgcgctcg ctgtttgaaa cgcttaaacg cgatatcgcc
      241 agcgtttctc cagaaacaga agtggtgagc gttgagattg aactgcataa cccgtgggat
      301 ttcgaagagg tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa
      361 aaagaagact atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt
      421 ctgctggcgg aagcgcgtta cctgcccgcc cggttgatac aatcttcacc tccgcgcaaa
      481 aaagaacagc cgcgcggcgc aggtgaagtg acgattatcg atctcgattt aagccgttat
      541 aacgccatcg ccagccgctt tgccgaggaa cgccagcaaa cgcttgattt tcttaaatcc
      601 ggcattgcca cgcgtaaccc gcacttcaac cgcatgattg aacagatcga aaaagtggcg
      661 attaaatccc gcgcaccgat cctgctaaac ggcccaaccg gtgcgggcaa gtcatttctg
      721 gcgcgacgta tatttgaatt aaaacaggcg cggcatcagt ttggcggcgc gtttgtggaa
      781 gtgaactgcg caaccctgcg cggcgatacc gccatgtcga cgctgtttgg tcatgtgaaa
      841 ggcgcgttta ccggagcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga
      901 atgttgtttc ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgcttaaa
      961 gccattgaag aaaaaacctt ttatccgttt ggcagcgatc gccaggtgag cagtgatttt
     1021 cagcttatcg ccggaacggt gcgtgatttg cgccagctgg ttgccgaagg aaaatttcgc
     1081 gaagacctgt acgcgcggat caatctctgg accttcaccc tgccggggct acgccagcgc
     1141 caggaagata ttgaacctaa cctggattat gaagtggagc gtcacgccgc gcttaccggc
     1201 gacagcgtgc gttttaacac cgaagcgcgg cgcgcctggc tggcctttgc gacatctccc
     1261 caggcaacat ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggcgacg
     1321 tttgccacga gcggacgcat tactctggaa gtggttgaag atgagataaa ccgtctgcgc
     1381 tataactggc aggagagccg cccctccgcg cttacgcagc tactgggcgc agaggcagaa
     1441 aacatcgatc tcttcgaccg tatgcaactg gaacacgtta tcgcaatctg ccgccaggca
     1501 aagtcgcttt ccgcagccgg acgccagctt tttgacgttt cgcgccaggg caaagccagc
     1561 gtcaatgacg cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc
     1621 gtgcaggatc agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt
     1681 actggcggcg gggcgtcggt gtagacggca tctaccatgc tgatgctgcc catattgacc
     1741 atcgcgttac ggccaaattt cgagtgatcg acaaccagca taacgtggcg cgagttctca
     1801 ataatggcgc gtttggtgcg aacttcgtgg taatcgaact ccagcagcga gccgtcgcta
     1861 tcgatgccgc ttatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc
     1921 gtcgcttcgc caatgatccc gccatcgcgg ctgcgtaatt cgccaccggc gagaataatg
     1981 cggaaatctt cttttaccat caacgtgtta gcaacattga gattgttggt gacaatacgc
     2041 aaattgctgt gattaagcag cgcgtgcgcc accgcttccg gcgtggtgcc aatatcgata
     2101 aacagcgtcg agccgttggg gatttgctcc gccactttac gggcgatgcg ctctttttct
     2161 tcggtctggg tggccttgcg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc
     2221 gcaccgccgt gatggcgcag gatcaggttt tgctccgcca gctcattgag gtcgcggcga
     2281 atagtctgcg ggctgacgga gaaatgctct accagctctt cggtactgac ataaccctgc
     2341 tgtttaacca gttcgataat accgttgtga cgttgtgttt gtttcatttc taaatccctg
     2401 gaattatttt cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc
     2461 taacccggcg atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc
     2521 gacaatccag atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg
     2581 atcgcgttcg ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc
     2641 gccaaaccac ggcccgctga atttttgctg cacatagccg cttaacaggg cgctaatgag
     2701 agtaatgaca attagcttac cgctaccgag gcgtttttcc accgcaccgc cgagatacca
     2761 ccaccagagc aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa
     2821 gtaacgccag aactcaaatt tcagtgctgg atcgaatggc caggccagcc ataacatcac
     2881 ttcctgatcg ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac
     2941 ccaggttacc ggacctgcgc gttcacgcaa ggcggcaaag aaaggataac ggcgataatg
     3001 caggccactg ccggtatggc ctgcctgcca gctcgccgcc agataacgcg gatctgccgg
     3061 gttttcgaga aaacgcgcca gctccgcccg tacgcgctcg gcctgggact catccgccag
     3121 ccagacatcg ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta
     3181 atcaacaaac gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc
     3241 tgtcgcttat tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct
     3301 gcgggaaact gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag
     3361 ccctgttgca gcagatactg cgccgcgcct ttgctgctat taccgtggta acacatcacc
     3421 atcaccggtg tgtcaaagtc gttatcacgc ataaacgcgc ccagtgtgtc gttggttaaa
     3481 tggaaagcct gcaccgcatg ccccattgcg aaactctgtg gatcgcgaat atcgaccagc
     3541 actgcctctt tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga
     3601 tccatgcgtc tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga
     3661 agcggcagat aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac
     3721 ctaaagcgcg attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag
     3781 tgaatgaggg cagcatggaa accaaagatc tgattgtgat agggggcggc atcaatggtg
     3841 ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg
     3901 atctcgcttg cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc
     3961 ttgagcacta tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga
     4021 aaatggcccc gcatatcgcc ttcccaatgc gttttcgcct gccgcatcgt ccgcatctgc
     4081 gcccggcgtg gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca
     4141 gcctgccggg atcaactggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta
     4201 agcgcggatt cgaatattcc gactgttggg tagacgatgc ccgtctggtg ctcgccaacg
     4261 cccagatggt ggtgcgtaaa ggcggcgaag tgcttactcg tactcgtgct acttctgctc
     4321 gtcgcgaaaa cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata
     4381 gctggcaagc gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg
     4441 acgggatgca tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg
     4501 tgccgcgcgt gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgtattg
     4561 tgttcgtgat cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca
     4621 aaggcgatcc gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt
     4681 ataacacgca ctttaaaaag cagttaagcc gtgacgatat cgtctggacc tactccggtg
     4741 tgcgtccgct gtgtgatgat gagtccgact cgccgcaggc tattacccgt gattacaccc
     4801 ttgatatcca tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga
     4861 ccacctaccg caaactggcg gaacatgcgc tggaaaaact gacgccgtat tatcagggca
     4921 ttggcccggc atggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg
     4981 acgattacgc agctcgtctg cgccgtcgct atccattcct gactgaatcg ctggcgcgtc
     5041 attacgcacg cacttacggc agcaacagcg agctgctgct cggcaatgcg ggggcgataa
     5101 gcgacctcgg ggaggatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg
     5161 atcacgaatg ggtccgccgc gccgacgatg ccctgtggcg tcgcacaaaa caaggcatgt
     5221 ggctgaatgc ggatcaacaa tctcgtgtga gtcagtggct ggtggagtat acgcagcaga
     5281 agttgtcgct ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg
     5341 agactgatga caaacacaaa attgcctgat gcgctacgct tatcaggcct acgtggttta
     5401 tgcaatatgt tgaatttgca tgattttgta ggcctgagaa agcgttcacg ccgcatcagg
     5461 catgaacaaa gtgcactttg tcaacaatct gacctacttt ttgagtcaga gaattaccgt
     5521 aaagcccacg ccatcatact caacgtaaca aaactcaggc acgttcccca ctcactccac
     5581 ttccgtatct ggcttctatt tttgctacgc cactggcaat aatatcccaa cgcaaaacca
     5641 agctgtgcgc ttattggacc gacaaagtca atccacggtt gagaccaaaa tggcgaaaaa
     5701 ttcagtggga gccagacaac ggtcagaaag aaactgcgca tattttccag caatgcaatt
     5761 ttcgctggcg cgacatagaa aatgatattc cacaggagca accagaaata aactggtaca
     5821 aataccagcc aataatgcag tttgcttttc aattcagatt gagttatgcg agcaaattgc
     5881 caaccaacgc aacatatggc gcagaacaaa agcgtatatt cagccaataa cccaagttgt
     5941 aattcaagaa gggatatacg cgcaccatag ggagatatag aaaatagtaa ttcccccata
     6001 aaagtcaacg tggatataaa tatagctaaa gcaatcaccg tcggccatac caacaacggc
     6061 acaataacaa agccgagaat tagcggagaa atataaagtc tgattttatt catctttatt
     6121 gtcctggtga ttcggatgat ctgtcgtaca tgacacataa acactcatca atcatccact
     6181 tgtcttccca gacctgatac tgtgtgccct gttcggactg cttaagcggg ttctcaatcg
     6241 cctgccgcgc gcgttctttc aggatggatt gattgttgtc agcccaggta ttgatttggg
     6301 cggtaatggc attatcgata tcgtcctggt tatctctgat ataaccttca cgatactggt
     6361 tcaataaaca ggcaagtgaa gctgccgacc aaaagccggg aattgaatta acggcatcaa
     6421 gcaatggcgc aagagcgata tcggcacgga aaaacatttc cagcatatac tgtaattcga
     6481 cagcttcatc gccggatacc caatcagtca tatactggca ggcaaatcgc tgtaaaattg
     6541 cccgttcatc cgcacgccag ttttctttgt tggcaaggtt gatacggcgc agagaaaaaa
     6601 ctacgagaat attgtcaaaa tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat
     6661 agcggacctc atcgctattt tgcggatcag gccctgggct actattccag gcattgatta
     6721 aggaatacgg gatcgctcgt aacgatgtac tccgtaatgc cttttgttcc tgctgactca
     6781 ggcaatattc acaacaaacc acaaatttct gcggcaccgg ataacgacga aaaatatgat
     6841 aaatctgctc aattaatgcc tgcatggcgg ggctgggttg cataattacc ccctcgccag
     6901 ctgatcacgt gtaaacggtt gatagacaaa acgagcattc gccgcgttca ggcgaataag
     6961 ggcgctacgc gaaagggaaa agctggggcg aatgcaacta ttgcagtctc gacagctttc
     7021 ctctgcgcaa ttttccactg agtaatgacg cgtgtagagt ttcagttcgg cctgcttatc
     7081 gcgaaatccg cgtaaactgt tattgttatg aaatttccag gcatctttaa agacctgaaa
     7141 gatatagaca tcctcaatcc aggagtcaca ttttttaagc gggcgctttg ctattatcac
     7201 ccgctcatcc ggatttagca tcgccgtaaa ttgtacggtg acgtcatttg tcgtgttcag
     7261 gtcacattga acatctggta catcactgat ataggggtca aatccaaaat cagcaccaca
     7321 ggcacaaaga cggctaatgg tggcggtatc gagggaaaat cccggccata ccttgctgga
     7381 cgttaagcca atatttaatg tgacatcata tcgaagcaat ccctcaagac cgtcagcacc
     7441 gacgaacttt tccccttcat ccaggacctg gttaataagt tgctgaacaa tgccttcatc
     7501 ccagtatgcc gatttgcagt tggcaatcgt aatatccacg cctgtgaagg gatcagtacc
     7561 ggcaaggctt gcaaataatg tattaactaa ttccattttt attatccagc tttaaggtag
     7621 cgtcttatac atcgggacat aacctacatc ttccaccaaa ctctgcccct gcggcgttaa
     7681 aaaccattcc accaattttt gtgtctctga cgtcgtattt tccctcgtta ccataaaggc
     7741 atcgacgata tagggatatt tgccgttgcg aatattttcc gcagtcggtg caataccgtt
     7801 aatcgctagc agttttatat ttttatcggc gttcatttgt gttgcgtaat aacggaaggt
     7861 gtaacctatt gcgttgtttg tattacgata ttcggcaacg actttaatca ttccctccat
     7921 tacgcttgcc acttcggttt cctgcggcga gatcatgcgg acatttttca tgacctgtga
     7981 ttgcatcact gtctggctgc cagagtcttc cgggcgctgc caggtctgga tctcctgatc
     8041 gttaccgcca acagtacgcc aattggtaat tgcaccactg aaaatgtcac gcacctgttg
     8101 ttcagtcagg gaattaaccg gattatccgc attgacgatg aaaacgaatg cttcacgggc
     8161 aaagggggtg tacagcaaag tgacaccaga ttcctttgca cgtttttttt gcccaccgga
     8221 aggttgcgcc acgaaaataa tatcagcatc gcccttaacg attttgttat atgcttctgg
     8281 cgtacgagag ttttccagat aatcccaaac gtgaaaatcc tctggtatta cacttaatgc
     8341 ataaaatgca gaagcataaa ttgggtacgc agccgtggcg ccatcgattc gcggccagtt
     8401 ttgcctaaac tgaatttgcg gtttgccacg cagcggcgtc agtcgattat tgagtttatc
     8461 gggttgccag gcccagatat caatattttc gcgtacaccg acaccaggat tgagctgttt
     8521 atataaataa ccctgataac cagcgacaaa accaagcatg agaaggataa acaggaggga
     8581 attacggcaa cgtagcccgg atgtaccggt taaaggatgt ctggttggcc ggccgtaccc
     8641 cagcgaaaaa ctgatatgcg cgccaacggg tataatgact atccaccagt actcacctcc
     8701 gactactgag actaacaaaa cgcccagcca ggggagcata agatcagcca accaacccga
     8761 tcccatctgg tgaaattgat aatctgccag atgcagcgcg attatccagc caaccaggat
     8821 taataaagcg ggtactaaaa aaagacattg tcgccagacg gctatttctc ttttttctgg
     8881 tgcactatcg cctgcccata ctcgccccga gaaaaatagc attaatgcgc ccagcgccag
     8941 cataaaaatg ttgagcagaa ggtaaattgt tggcgtgata aggatattac aaacttcaat
     9001 aataccggca agtccgacac ccagcatagc aacaaccgcc gccaaaaatt gcgtcagtat
     9061 ggggatgccg aaaaaagtca ttaccagcga ggacaaaatc cattttctgt tttgcattat
     9121 tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc cccttctgga
     9181 cacttttccg aaatgatggc gaaaaaaacg ggaccctttg gccccgttct atttattggt
     9241 gaaattacaa tctcaccgga tcgatatgcc agatatgatc agcgtactct ttgatagtac
     9301 ggtcagaaga gaagtagccc atattggcaa tgttcagcat cgcttttgcg gtccactctt
     9361 cctgaagctc gtagagttca tcgactttat cctgacaatc gacatagctg cgataatccg
     9421 ccagtacctg gtagtgatcg ccgaagttga tcagcgaatc aaccagatcg cgatagcgac
     9481 ccggatcttc cggactgaat acaccgctgc cgatttgcgt cagcacctga tgcagctcct
     9541 catctttctc gtagtattca cgcggtttgt agccctgacg acgcagttct tccacttctt
     9601 ccgctgtgtt accaaaaata aagatattgt cagcaccgac atgatccagc atctcgacat
     9661 tcgcaccgtc caacgtaccg atagtcagcg caccgttaag cgcaaacttc atattactgg
     9721 tgccggaagc ttccgtccct gccagcgaaa tctgttcaga cagatctgcc gccggaatga
     9781 tcaactgcgc caggctaacg ctgtagttcg ggatgaacac gactttcagc ttatcgccaa
     9841 tctgcggatc gttgttgatc actttcgcta cgtcattgat caaatggata atatgcttcg
     9901 ccatgtaata ggccgaagcc gccttaccgc caaaaatatt cacgcgcggt acccacttcg
     9961 catccgggtc ggccttgatg cggttatagc gggtaatcac atgcaacaca ttcatcaatt
    10021 gacgtttgta ttcgtgaata cgtttgattt gtacatcgaa caacgccttt ggattcacca
    10081 ccacattcag ctgctgggcg atatactctg ccagacgctt tttgttctcc agcttcgcct
    10141 gatgcacagc gtgattaacc attgggaaat cacagtgttg ttgcagctca ttaagcaggc
    10201 taaggtcggt gcgccagtta cggcccaggt gttcgtccag cacggctgaa agcgatgggt
    10261 tcgctaccgc caaccagcga cgcggcgtca caccgttggt gacgttggtg aaacgacccg
    10321 ggaagatttt cgcaaagtcg gcaaacaacg attgcaccat cagattagag tgcagttccg
    10381 atacaccgtt aactttgtgg ctcacaacaa ccgccagcca ggccatacgc acacgacgac
    10441 cgttggattc atcaatgatc gacgcccgtc ccagcagatc ggtatcgttc ggatactgtt
    10501 cctgcaaggt tttcaggaaa tagtcgttga tttcaaagat gatctgcagg tgacgcggca
    10561 gaattttacc cagcatatca accggccagg tttccagcgc ctcgctcatc agcgtgtggt
    10621 tagtgtagga gaagacctga caacacacct caaacgcgtc gtcccagcta aattggtgct
    10681 catcgatcag cagacgcatc atctcaggaa tcgacagtac cggatgggta tcattgagat
    10741 gaatcgcgat tttatctgcc aagttatcgt aggttttatg caactgataa tggcggctta
    10801 aaatgtcctg aatggtcgag gaaaccagga agtattcctg acgcaggcgc agctcacgcc
    10861 cggagtaggt ggagtcatcc ggatacagta cgcgagatac gttctcggag tggtttttat
    10921 cttccactgc cgcgaagtag tcaccctggt tgaatttacc gaggttaatt tcgctactgg
    10981 cttgcgcact ccacaaacgc aacgtgttgg tcgcgtcggt gtcgtaaccc gggattatct
    11041 gatcgtaagc gactcccaga atctcttcgg tttcaatcca gcgcgttttt ttaccttcct
    11101 gctgaatgcg accgccaaaa cggactttat aacgcgtgtt gtggcgtttg aattcccacg
    11161 ggttaccgta ttccagccag tagtcaggcg actctttctg gctaccgtta acgatgttct
    11221 gcttgaacat accgtagtca tagcggatgc cgtaaccgcg ccccggcaac cctaacgtcg
    11281 ccagagaatc gaggaagcaa gccgccagac gtcccaggcc accgttaccg aggcccgggt
    11341 cattttcttc atcaatcagc tcttcgagat ttaaccccat cgcttccagt gcgccctgta
    11401 catcttcgta aattcctagc gacaacatgg cgttggagag cgtacggcca atcaaaaact
    11461 ccatcgacag gtagtaaacc tgacgagttt cttgcgacaa ctgggcacgg tttgaacgta
    11521 accagcgctc cacgagacga tcgcgcacag caaataacgt tgcgttcagc cattcatgtt
    11581 tattggcgac gaccgggtcc tttccaatcg taaacatcag cttgtaagcg atagagtgct
    11641 taagagcttc tacgctaagc gtgggcgatg aatatgtaaa cggagcattc atataggcgt
    11701 ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta cgacttcgcc gcgacctgcc
    11761 agctaaaatc cattgccata gcctgacgtt gcacaaaccg ccacagtgaa ggacgggacc
    11821 acagtacaaa agcacgtcga atagcccgta acagcgacca ggcattacta tcttcaaaga
    11881 caaacccact ggcgacgcca tctgcaaggt tctcaagaga acagtcagaa accgtatcag
    11941 caagcccacc ggtgcgccgc actaacggca gcgtaccgta cttcaatcca taaagttgcg
    12001 ttaagccgca cggttcaaaa cggctgggca ccagaatgac gtccgcgccg cccataatgc
    12061 gatgcgaaaa tgcttcgtga tagccaatct gaacgcccac ctggccgggg tattccgctg
    12121 ccgccgcaag gaaaccttcc tgcagcaccg gatcgcccgc gccgagtagc gccagctgcc
    12181 cgccctgctc cagaaggccc ggtaaggctt ccagcaccag gtcgagacct ttctggctgg
    12241 tcagacggct caccactgca aaaagcggca ctttatcgtc aaccttaagc cccattgcga
    12301 tttgtaactg gcgcttattt tccgctttat cttccaacgt atcgcgggtg taacgcgagg
    12361 ccaacagtaa gtccgtctct ggactccaga ttttctcgtc cacgccgttc agtacgccgg
    12421 aaagacgccc ttcacggtga cgctgttgca acagaccttc cataccgtag gcaaactgcg
    12481 gttcggtgat ctcgcgagcg taggttggac tgaccgccgt aatgtgatcg gcatagtaca
    12541 gaccggcctt caggaaagag atttgtccgt tgaattccag cccatgaata ttaaagaatg
    12601 accatggcaa ttggatgtca ttcatgtgat gtgcataaaa catgccttga taggccaggt
    12661 tgtgcacagt aaacaccgac ttcgccggac gcccgcgcgc cgccagatat gcaggcgcaa
    12721 ggcctgcatg ccagtcgtgc gcatgcacca catcaggacg ccagaatggg tcaagcccgc
    12781 tggccatttc tgccccaacc caccccagca gcgcaaaacg caatacgttg tcggtatagg
    12841 caaataagtt agtatcgtga tacgggcttc ccggacgatc atagagatgc ggcgcgtcaa
    12901 tcaggtatat gccaaccccg ttgtaatgac cgaacaacag cgtgatatgt ccggcgaagg
    12961 tatcacgacg ggatactacc tgcgcatcgg tcacgccacg gcgaatatcg ggaaatgcag
    13021 gcaacagtac gcgagcgtca acgccgtctg cgatttgtgc tgcgggtaat gccccaataa
    13081 catcagccag accgccggtt ttaagcagcg ggaacatctc tgaacataca tgtaaaacct
    13141 gcattatcgc tcctgtttat gccctaactt ccgtagcatt tcgcgcgtta ccagcacgat
    13201 gccttcttct gaacgataga aacgacgtgc atcttcctct gcgttttcac caatcaccat
    13261 gccttccgga ataacacaag cacgatcaat gacgcagcgg cgcagacggc acgagcgacc
    13321 tacccatact tccggtaaca atacggcgga atcaatgttg cagaatgaat tcacgcgaac
    13381 gcgcgagaac agaacggact gcaccaccac cgaaccggag atcacacaac cgccggaaac
    13441 cagtgagtta agggtcatcc cgtggctacc ggagcgatcc tgcacgaatt tcgctggcgg
    13501 taatgattca ttgtaggtgc gaattggcca attgcgatcg tacatatcca gctccggtac
    13561 cacagaggcc agatcgaggt tcgctttcca gtaagcttcc agcgtaccca catcgcgcca
    13621 gtacggctcg gcatccgggt cggattgtac gcaagagagc gggaacgggt gcgcataggc
    13681 cagaccggct tcggtgatct tgggaatcaa atctttgcca aagtcgtggc tggagttctc
    13741 atcgcgatcg tcttcttcca gcagttcata cagatagtcg gcgtcaaaga cgtagatacc
    13801 catactcgcc agagatttgc tcggatcgtt cggcattgac ggcgggttag caggtttttc
    13861 aacgaattcg ataattttat cgttctcatc aaccgccata acgccaaatg cggaggcttc
    13921 ttcaatcggt actggcatac aagcaacggt gcaacgtgcg cctttttcga cgtgatcgat
    13981 aagcatacgc gagtagtctt gcttgtagat atggtcgccc gccaggatca ccacgtattc
    14041 cgctttataa cggcggataa tatcgaggtt ttgggtgacc gcatctgcgg tgccgcgata
    14101 ccagttttcc cctttcattc tctgctgtgc tggcagcaga tcgacaaact cgttcatttc
    14161 ttcattgaag aatgaccagc cgcgctgaat gtgctgcacc agagtgtggg actggtactg
    14221 ggtgatcacg cccatacgac ggatcccgga gttgatgcag ttagacagcg caaagtcgat
    14281 aatgcggaac ttaccgccga agtgtacggc cggttttgct cgcttattgg ttaaatcctt
    14341 caggcgggta ccacgtcctc ccgccagtat cagggcaaca gatttcaatg gcagctggcg
    14401 cgccaacatt aagtgatcgt tcttctctaa actaaccatg actaactcct tttttatcat
    14461 ctctggaaca cacacaatcc gtgtgcaggt ccctgccaga cagccgtaat cactgggtta
    14521 tcctctccag cgaatggggg aatggcgtgc cactccccag caggtaaaac aatctctgtt
    14581 acctcaagcg tggcgttaat tgcgatcaaa aagcgatccg agagcagaat ttgcagctgt
    14641 ttcggcccgt tttgccactc atccgtgctt aaaggttgag catatcgatt tagccaacgg
    14701 acattgccgt cgccttcttc ccaccagcga ttctccacca aagcgggaat acgcttgcgc
    14761 agatggatta acgcggcggt aaatgcggtt aaaccactgc ttgcctgcga ccagtccaac
    14821 caggttaatt ggttatcctg acagtaggca ttgttattgc catgctggct gtgaccatgt
    14881 tcgtcaccgg ccagtaacat cggcgtgccc tgggagagca acaacgttgt taacagggca
    14941 tgaatgctgt cgcgccgccg ttcaaccaga tcaagagtac cgcctaaccc ttctttacca
    15001 tgattgttac tgtaattgtt gttggtcccg tcgcgatttt cttctccgtt tgcttcattg
    15061 tgtttatggt tgaagcaaac gcagtcgcga agcgtaaaac cgtcatgtgc ggtgacgaga
    15121 ttaatcgcgg cactcggcag acgaccatta cgtttaaaaa catcgctgga ggcagcaaaa
    15181 cgcccggcaa acgcccccag aggcaaatca taatgcagcc agaaacgacg ggcagcatcg
    15241 cggaaatgat cgttccactc ggcaaacagc ggcgggaaat ttcccacctg ataaccacca
    15301 ggagcgatat cccacggttc agcaattaac ttcacctgcg agagcaccgg gcagttctgg
    15361 attgcggtaa acaacggcgc atcctgacgg aactctggcg tacggcccat gactgccgcc
    15421 agatcaaagc ggaaaccatc gacgtggcag gtttctaccc aataacgcag gcaagcgctg
    15481 gcataatcca ccaccgccgg gtgactcaaa ttgagcgtgt tgccacaacc ggtccagttg
    15541 tgataatcgc cgtcttctct tatccaataa tagctacggt tgtcgatccc acgcagcgag
    15601 aataacgggc cgtcgaggtc cagttccgca ctatggttga gtacgatatc aagaatgact
    15661 tcgatacccg ctttatgcag tgctttgatt gcatcgcgaa actcatccag cgccgtttct
    15721 ggcgagcagg catacgccgg atgcagcgca aacatcgcca ccgggttgta accccagtag
    15781 ttacttagcc ccatgcgttg cagacgtggt tcactggcaa actgcgccac tggcagcagt
    15841 tccagcgcgg taatgcccaa ttgtttcaaa tagttgatca tcaccggatg cccgagggct
    15901 ttataagtgc cacggatctc gaccgggatc tccgggtgca ggtacgttaa tcctttaaca
    15961 tgggcttcat aaatgatggt gctgccccac ggcgtgcgcg gcggggcatc atcttcccag
    16021 tcatagtgat caaccaccac tacgcatttc ggcgcaatgg cggcgttgtc gcgatagtca
    16081 ggttcattat gaccggcgtg cagcagcggg ttatctttaa attccccgtc aatttgccgc
    16141 gcgcaaggat caatcaacaa cttcgccggg ttaaagcgat gcccctcggc gggttgccag
    16201 gggccatgaa cgcgataacc ataacgcaaa cccgggcgcg catccggcag ataaccgtgc
    16261 caaatgtcgc cactgtgccc tggcaagtca tagcgatgtt cctggccatt ggcgtcaaag
    16321 acacacagct ctacccgctc ggcatgagcg gagaaaagtg tgaagttgac gccctgaccg
    16381 tcgtaatgcg cgccgagggg agcgggcttg ccaatggcga gttgtgtcat tctgcctccc
    16441 gaaccagcca gatagtggcc agcggtggta gcgtcaggct tagtgaatgc tgacgaccgt
    16501 ggctggcaat ctcatcgctg tgtaccgtgc cgccattgcc tgcattactg ccgtgatagt
    16561 gcatggaatc ggtattgagg atctcacgcc atttgcctgg ctgattaatg ccgaagcgat
    16621 aatcatgacg cggcaccggc gtaaagttac tggcaacgat gatttcgtta ccctctttat
    16681 cgcgacgcac aaagatcagc accgagcgtt ctttgtcatc caccaccagc cattcaaagc
    16741 cgtacgggtc aaaatccagt tcatgcatcg ctttatggtg gcggtaggtg aggttcagat
    16801 cgcgcaccag acgctggaca ccgtggtgcc agttatcgcc gccttccaac agatgccagt
    16861 cgaggctggc gtcatggttc cactcgcggc cctgggcaaa ttcgttcccc atgaacagca
    16921 gtttcttgcc cggaaatgcc cacatccagc catagtaagc gcgcaggtta gcgaatttct
    16981 gccatgcgtc gcccggcatc cggtcgagaa tcgatttttt accgtggacc acttcatcat
    17041 gcgacaacgg caggacgaag ttttcagtgt agttgtagag catcccgaag gtaagtttat
    17101 cgtgatgata ctgacgataa atcgggtcga gtttcatgta gtccagggtg tcatgcatcc
    17161 agccgaggtt ccacttgtac cagaagccca gaccgcccat atcctgcgga cgagaaacgc
    17221 cagggaaatc ggtagactcc tcagccattg tcaccgcacc ggaaacctgc tcaccaagaa
    17281 tacggttggt attacgcaag aattcaatcg cttcaagatt ctcgcgcccg ccaaattcgt
    17341 tcgggatcca ctccccctct ttacggctgt agtcgcgata aatcattgac gccaccgcat
    17401 cgacgcgcag cgcatcaata ccaaaacgtt caatccagta aagcgcgtta ccgacaagga
    17461 agttactgac ttcacggcga ccatagttgt agattagcgt gttccagtcc tgatgatagc
    17521 cttcacgcgg atcgctgtgt tcatacaagt tcgtgccatc aaattcggca agcgcaaagt
    17581 catcagtcgg gaagtggcct ggcacccagt cgagaatcac gttcagacca gctgcgtgtg
    17641 cggcatcaat gaaataacgg aagtcgtcgc gagtaccaaa acggcgggtc ggtgcataca
    17701 ggccggttgg ctgataaccc caactgccat cgaagggatg ctcgttaatg ggcagtagtt
    17761 cgaggtgggt aaagcccatc catttagcat aaggcaccag ttgatcggcc agctcgcggt
    17821 agctcaacca gaaattgttg tcggtgtggc gacgccagga gcccaggtga acttcataaa
    17881 tagagattgg cgcatcaaac tgattcgctt ttttgcgctc ttcagtctgt acaacctttt
    17941 ccggcagccc gcaaataaga gacgcggttt ccgggcgcat ttgcgcttcg aaggcataag
    18001 ggtcggactt cagacgcaag ttgccattgg catcaatcat ctcgtattta tagagctgac
    18061 cgttatgcgc cccagggata aacagttccc agatgccgct ctctttacgc aggcgcatcg
    18121 ggtgacggcg accgtcccag tagttgaatt gcccaaccac cgagacccga cgggcgtttg
    18181 gagcccagac agagaaacgc gtacctgtga cgccatccat agtatctgca tgcgcgccta
    18241 aggtttcata cgggcgcagg tgagtacctt cagataatag ccaggcatcc atttcctgga
    18301 ttagcggacc aaaacggtaa ggatcatcaa tcaggttttg ctgaccatgc cagacaacag
    18361 ccaactgata gcggaaaaaa ttcttacgtc gcggaatgac gccgctaaag aatccccgtg
    18421 agtcgagaca ctccagtttt gcgagtttgc gcccggtttt cggttcaatc acccacacat
    18481 cggtagcgtc gggtaaaagg gcacggactt ccagtcccgc ggtggtttta tgcattccca
    18541 gtacggaaaa aggatccgca aaatggcctg caattagcgc gttaatcacg tctctatcga
    18601 tacgatcgga catgcttgtc ttcctgtttt attgtgtcac cccatccaat tggatttttg
    18661 acttctggtt gtgacatttt tttgacctga acggcgcagc actctgtgca tcctctctgc
    18721 gtcgtcctca cttcaggtaa ggctgtgaat actcatgtat tcagccaccc ttaaagaata
    18781 gccaatgctc tatttaactc ccggtaaatc atgaaacatc tgcgcttact cctgtattac
    18841 gcactaacag gggcggcatc gcgccccaga tttaatgaat aaagattacg ccagttgacg
    18901 aagcatccga cgcagcggct ccgcggcccc ccacagcagc tggtcgccca cggtaaaggc
    18961 tgacaggaac tctggtccca tattcagctt acgcaggcgg cctaccggcg tggtcagcgt
    19021 gccggtaacg gcagctgggg ttagctcacg catagtgatt tcccgatcgt tcggaacgac
    19081 tttcgcccac ggattgtgcg cagccagcag ttcttccacg gtcggaatcg acacatcttt
    19141 tttcaattta atagtgaatg cctggctgtg gcagcgcaat gccccgacac gcacacataa
    19201 accatctacc ggaattacgg aagatgtgtt gaggatcttg ttggtttccg cctgcccttt
    19261 ccactcttcg cggctctgac cgttatcgag ctgtttgtcg atccacggaa tcaggctacc
    19321 cgccagcggc acgccaaagt tatccaccgg cagctcaccg ctacgggtta aggttgtgac
    19381 tttgcgttcg atatcgagaa tagcagagga cggggtcgcg agttcatctg ctacatggcc
    19441 atacagatgg cccatctggg ttaataactc acgcatatgt cgcgcaccac cgccggaagc
    19501 ggcctggtag gttgcaacgg acacccaatc aacaagatca ttggcgaata aaccacccaa
    19561 cgacatcaac atcaggctta cggtacagtt accgccaaca aaagtcctga tgccattatt
    19621 taatccgtcg gtaatgacgt cctgattgac ggggtcaaga atgatgatgg cgtcatcttt
    19681 catgcgcaga gacgatgctg cgtcaatcca gtaaccctgc catccgcttt cacgaagctt
    19741 tggatagatt tcgttggtat aatcgccgcc ctgacaggtc acaatgatat cgagggcctt
    19801 tagcgcctcc agatcaaagg catcctgaag tgtgccagtg gttccgccaa aagacggcgc
    19861 agcctgacca agctgagaag tagaaaagaa gacagggcga atggcgtcga agtcgcgctc
    19921 ttcaaccatg cgttgcatga gaacggagcc gaccataccg cgccagccga taaaaccaac
    19981 atttttcata agcgtttttt cctgcaaaga tgtgtgctgt ataaatgtgc cggtctcctc
    20041 ttggcacatc tttcaccata caaaaagcag ccaaagtcgc aagtgaaatt aatcaatgat
    20101 agcgaagcca tcagtaatgc gacttatcct gctttgttag cacgcagaaa gtccgcggca
    20161 attatcaggg aatttgagtt atgaatgaaa tcatttctgc agcagtttta ttgatcctga
    20221 ttatggatcc gctcggaaac ctacctattt tcatgtccgt actgaaacat actgaaccga
    20281 aaagacggcg ggcaatcatg gtgcgagagt tgcttattgc tctcctggtg atgctggtgt
    20341 tcctgtttgc gggtgagaaa attctggcat ttcttagcct gcgagcagaa accgtctcca
    20401 tttctggcgg catcattctg tttctgatcg ccattaagat gattttcccc agcgcttcag
    20461 gaaatagcag cgggcttccg gcaggtgaag agccatttat cgtgccgttg gcaattccgt
    20521 tagtcgccgg gccgactatt ctcgccacgc tgatgttgct gtctcaccag tacccaaatc
    20581 agatgggaca tctggtgatt gctctgctgc tggcttgggg cggcaccttt gtcatcctgc
    20641 tacagtcttc gctattttta cgtctgctgg gcgagaaagg ggtgaacgca cttgaacgcc
    20701 tgatgggatt gattctggtg atgatggcaa cccagatgtt cctcgacggc attcgaatgt
    20761 ggatgaaggg gtaacgagga gagtttttcg aaaagagccc ggtagcgtca caactaccgg
    20821 gcgaacaaac ttaactcaac agctggaacg caatcatccc aacgatggca ccgacagtgc
    20881 cgaggatggt ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa
    20941 atttaccgaa caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg
    21001 cgatacaaat cgacagcgcc gccatttgcg caccggagta gttcagttgt tcaatgactg
    21061 gcatcaccag tcccaccgcc gttaaacagg caacggtggc agaaccctga atgatgcgca
    21121 ctgcagcggc cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt
    21181 cgcccagtgc cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcccgcac
    21241 caatcaccag cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt
    21301 tgtcttttgg catgccctga cgcattgcca ggccataaat cactaccaga caagcaacca
    21361 gaattgcggt aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg
    21421 gcacaaaacg cgcggcaatg gttttcagcc ccaccagcac cagcggcagc aggatcagcg
    21481 acaggctgaa tccgaaggac ggcattttgc cttcgccgag atgcggctcg ctgatgtcgt
    21541 caggaatatg caactccacg tagcggctga tgaagttacc ccacagcggc ccggcaataa
    21601 tcattcccgg aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca
    21661 tctgcgatgc cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca
    21721 cgcctgcgaa taatgggatt accagcttca ccaggttcgt accggtgtgg cgcgccattg
    21781 agaaagcaac gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga
    21841 ccagccccgc aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttaagcattt
    21901 tgacggcaat ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca
    21961 gggcgacaac caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg
    22021 cgattttatc gagcggcata ccggaaaaaa ggccagcccc catggacacc accattaaag
    22081 ccaggaaagc gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta
    22141 ctgctgttaa aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa
    22201 tggtgcttgc cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt
    22261 cgtccgcacc cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga
    22321 aatggccttt gcgcgctttc aggcggcttt caatcacatc aaaatcgcct ttcaggtaga
    22381 tgaaagagag attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag
    22441 aacagacgat cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg
    22501 cctgcaacca cggtttgcga tcatcgtcat tcagcggttc gccagacgcc attttttcga
    22561 tattgcgccg tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca
    22621 cttcacttgc gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt
    22681 gatcatggtt agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg
    22741 ggtaactgtt atcggcaaca ttgtccagcc ggacattatc agaagcaata tccatccgtg
    22801 ccttaagtgt ataagtgtga gctacttcaa atttgtgggc ttaaatagat ccgcccggtg
    22861 acaaggtgaa acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca
    22921 gcaggcgttc agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgcaagtc
    22981 gtggctccat cacctgacca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag
    23041 gaacttttaa ccccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcattag
    23101 tacagaacac gccatccagc tgcggatatt ctcgccgcgc ctggcgaatc agttcaatac
    23161 cggaagagta agaagaagat tgctcaacca tcacgctata cggcaccagg cctgcatcca
    23221 gcatcgcctg ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgtgcgccga
    23281 gataggcaat gtggcgatgg ccgcgagcaa taatggcggc ggtcatctgg cgtgccgctt
    23341 caaagttatc aaaaccgacg gcgatatcga ggcacggcga cttgctgtcc atcagttcca
    23401 ccacgggaat acccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg
    23461 tgaggataag tccgtcgata ttccatgaga gcattgattc gagacgttct tgctccattt
    23521 ccggtttata accgtagtgc gccagcatgg tctgataacc gtgcgcgtcg gtgacacttt
    23581 cgattccgcg taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa
    23641 tcgcccggct ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt
    23701 catcaagagc cgcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta
    23761 aaaaacggct gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg
    23821 gtcttttctt tttcatcgtc ctgaagatac aaaagagata gattctctta gtttaacacg
    23881 gacgcatcag aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta
    23941 aatcaattgc atcgcattgt gctaacgacg cggcaaaacc ccggaagcgt acatcagcac
    24001 gcgaccggag cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagctt
    24061 taaaccggcg gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt
    24121 gcctgctcat cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg
    24181 acattgcctt tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac
    24241 tgctcatctt tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa
    24301 ccatctcgcg catctggcga aagcaccagt tgtttgccct gtacggcatc gaagcgacgc
    24361 tgttcataac gcggcgtaat accattttct tcaggcatga tccagatctg atacagatgc
    24421 agacgctcgg tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata
    24481 atctggaact cgcccgccgg aacctgctct ttattgccca tgctgtcctg gtgctcaaca
    24541 gtaccttcca gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc
    24601 tgccctgctt caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc
    24661 ggatcgtaat agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca
    24721 tgaccacgtt catttgcttt gcgtaagtag atcatttgta ttcgccccct gaatgatttc
    24781 gatgggctta gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct
    24841 gttcaaaaaa attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg
    24901 agggagaggc ttgctcgaat ccgcgttcaa ggatttccag attcgtaaga acttcagatt
    24961 ccttgacgta atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc
    25021 cgtaatcgcc catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat
    25081 actccagcac accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag
    25141 ccttcaggct ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt
    25201 gaacgataaa tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca
    25261 ggtcgccata aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc
    25321 tgcggatgtc ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg
    25381 tatgcacacc aaggccatag aacgcgccat cctgcggcag cccaggtttg gtttctgcca
    25441 ccgggcggta atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt
    25501 caatcgcttt tttcgccgtc aggaagcagg aatcaaagcg acgattctga tacggcgtga
    25561 cggtcagccc tttgcttttc gccagtgcaa acagctcctt cgcctgggca agcgtcgggg
    25621 tgaacggttt ttcgaccagc acatttttcc cggcttccag cgcgcgtttc gcgtactcga
    25681 aatggctgtc cgcgtgggtg cagacaacaa ccagcttaac atcgggatcg tttagcactt
    25741 cgtcgagatc gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg
    25801 catggcgacg aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca
    25861 gatggtaacg ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt
    25921 cgtccttttt aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag
    25981 tgcgcataac gaacacaagc ccccccgtgg ataaattgag aacgaaagat caaaaaaaaa
    26041 gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg
    26101 ttctccgcga gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc
    26161 caacactttt tgcaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg
    26221 gacatttacc ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca
    26281 cgggattacg aggccattcg gcagattcac gcccagccag aggtgtatta caacacgcta
    26341 caggtgcctc atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc
    26401 aaacagttag ttgcctgtat tgatggaacc gtcgtgggtc acctcaccat tgacgtgcaa
    26461 cagcgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgactc ccgttggaag
    26521 aatcgcggcg tcgccagcgc ccttatgcga gagatgattg aaatgtgcga caactggttg
    26581 cgggtagatc gtatcgagct aacggtgttc gtcgataacg cgccagcaat taaggtctat
    26641 aaaaaatacg gctttgaaat tgaagggact ggtaagaagt acgcattgcg taatggtgaa
    26701 tatgtcgatg cgtattatat ggcgcgggtg aagtaagata gtgccctttt tctgagatgg
    26761 aaaaagggtg tcattcaaaa tcggcatacc ttc
//
LOCUS       DAZUGK010000042        26671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZUGK010000042 DAZUGK010000000
VERSION     DAZUGK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.042"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..286
                     /locus_tag="ACHY5W_001581"
     CDS             59..286
                     /locus_tag="ACHY5W_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446497.1"
                     /translation="MACNVFAFSISGGGSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKASYLIHPVR"
     gene            complement(363..605)
                     /gene="yfdY"
                     /locus_tag="ACHY5W_001582"
     CDS             complement(363..605)
                     /gene="yfdY"
                     /locus_tag="ACHY5W_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFS4446498.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            958..1878
                     /gene="lpxP"
                     /locus_tag="ACHY5W_001583"
     CDS             958..1878
                     /gene="lpxP"
                     /locus_tag="ACHY5W_001583"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFS4446499.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFCSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2234..2305
                     /gene="ypdK"
                     /locus_tag="ACHY5W_001584"
     CDS             2234..2305
                     /gene="ypdK"
                     /locus_tag="ACHY5W_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFS4446500.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2370..3608)
                     /gene="alaC"
                     /locus_tag="ACHY5W_001585"
     CDS             complement(2370..3608)
                     /gene="alaC"
                     /locus_tag="ACHY5W_001585"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFS4446501.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3985..5682
                     /gene="ypdA"
                     /locus_tag="ACHY5W_001586"
     CDS             3985..5682
                     /gene="ypdA"
                     /locus_tag="ACHY5W_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFS4446502.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYHNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5697..6431
                     /gene="ypdB"
                     /locus_tag="ACHY5W_001587"
     CDS             5697..6431
                     /gene="ypdB"
                     /locus_tag="ACHY5W_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFS4446503.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6444..7301
                     /gene="ypdC"
                     /locus_tag="ACHY5W_001588"
     CDS             6444..7301
                     /gene="ypdC"
                     /locus_tag="ACHY5W_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4446504.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGNFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7304..9793)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001589"
     CDS             complement(7304..9793)
                     /gene="ptsP"
                     /locus_tag="ACHY5W_001589"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4446505.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQSSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSVQPTQAELTAHP
                     LPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLATL
                     AEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQVC
                     AKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQFL
                     SLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCGVL
                     AINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPGAF
                     ANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKSIP
                     YLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQILW
                     VKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMTQY
                     LYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLLLG
                     LGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEEDVR
                     PLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVGFG
                     VAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQLA
                     RKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9818..10855)
                     /gene="ypdE"
                     /locus_tag="ACHY5W_001590"
     CDS             complement(9818..10855)
                     /gene="ypdE"
                     /locus_tag="ACHY5W_001590"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4446506.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSTMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10855..11940)
                     /gene="ypdF"
                     /locus_tag="ACHY5W_001591"
     CDS             complement(10855..11940)
                     /gene="ypdF"
                     /locus_tag="ACHY5W_001591"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4446507.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKAAFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11955..13202)
                     /gene="fryC"
                     /locus_tag="ACHY5W_001592"
     CDS             complement(11955..13202)
                     /gene="fryC"
                     /locus_tag="ACHY5W_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4446508.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13224..13550)
                     /gene="fryB"
                     /locus_tag="ACHY5W_001593"
     CDS             complement(13224..13550)
                     /gene="fryB"
                     /locus_tag="ACHY5W_001593"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4446509.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESHDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13770..14735)
                     /gene="glk"
                     /locus_tag="ACHY5W_001594"
     CDS             complement(13770..14735)
                     /gene="glk"
                     /locus_tag="ACHY5W_001594"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFS4446510.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14939..16195
                     /gene="yfeO"
                     /locus_tag="ACHY5W_001595"
     CDS             14939..16195
                     /gene="yfeO"
                     /locus_tag="ACHY5W_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFS4446511.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPASEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPNDNV"
     gene            16310..16636
                     /gene="ypeC"
                     /locus_tag="ACHY5W_001596"
     CDS             16310..16636
                     /gene="ypeC"
                     /locus_tag="ACHY5W_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFS4446512.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16777..18015)
                     /gene="mntH"
                     /locus_tag="ACHY5W_001597"
     CDS             complement(16777..18015)
                     /gene="mntH"
                     /locus_tag="ACHY5W_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4446513.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18351..19553
                     /gene="nupC"
                     /locus_tag="ACHY5W_001598"
     CDS             18351..19553
                     /gene="nupC"
                     /locus_tag="ACHY5W_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFS4446514.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19603..21792)
                     /gene="pdeA"
                     /locus_tag="ACHY5W_001599"
     CDS             complement(19603..21792)
                     /gene="pdeA"
                     /locus_tag="ACHY5W_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS4446515.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEVGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22000..22075)
                     /locus_tag="ACHY5W_001600"
     tRNA            complement(22000..22075)
                     /locus_tag="ACHY5W_001600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22040..22042),aa:Ala,seq:ggc)
     gene            complement(22115..22190)
                     /locus_tag="ACHY5W_001601"
     tRNA            complement(22115..22190)
                     /locus_tag="ACHY5W_001601"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22155..22157),aa:Ala,seq:ggc)
     gene            22411..22770
                     /gene="yfeC"
                     /locus_tag="ACHY5W_001602"
     CDS             22411..22770
                     /gene="yfeC"
                     /locus_tag="ACHY5W_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS4446516.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22772..23164
                     /gene="yfeD"
                     /locus_tag="ACHY5W_001603"
     CDS             22772..23164
                     /gene="yfeD"
                     /locus_tag="ACHY5W_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS4446517.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23204..24412)
                     /locus_tag="ACHY5W_001604"
     CDS             complement(23204..24412)
                     /locus_tag="ACHY5W_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS4446518.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKHIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYQQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTASADVNGARNILAAGHAVLACGEMVQSGHSLKQ
                     EPTEMIQATA"
     gene            24420..24850
                     /gene="tnpA"
                     /locus_tag="ACHY5W_001605"
                     /pseudo
     CDS             24420..24850
                     /gene="tnpA"
                     /locus_tag="ACHY5W_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(24962..26377)
                     /gene="gltX"
                     /locus_tag="ACHY5W_001606"
     CDS             complement(24962..26377)
                     /gene="gltX"
                     /locus_tag="ACHY5W_001606"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4446519.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALSFIAERENQQ"
     gene            complement(26490..>26671)
                     /locus_tag="ACHY5W_001607"
     CDS             complement(26490..>26671)
                     /locus_tag="ACHY5W_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446520.1"
                     /translation="KGGALPAELIPPAVWSRIIGRVQNESTHFLKKLFVRRKFKQDDR
                     KTDRVAQFLNENNNA"
BASE COUNT         6579 a         7045 c         6589 g         6458 t
ORIGIN      
        1 ataactttct atgaaagtaa acttaatagt ttttagctta tttttattgg tctctattat
       61 ggcatgcaat gtttttgcat tttccatttc tggtggtgga agtgagagga gctataaaga
      121 gacggaaaaa acatcagcga tgacgacaac tcactctaca aaacttcagc catcacaggc
      181 aattttgttt aagatgagag aagatgcgcc accattaaac ctcacagaag aaaccacgcc
      241 cccttttccg acaaaggcga gttatcttat tcatcctgtg cgatagcgtc tgtcacatgg
      301 caggtgagca atcatgtctg atgctgatgt gaacacctgc ccaaatgagc catcctgacc
      361 cactaccgcc tgccataccc caccataaag cgaatcatca agccacaagc cggggcaaga
      421 aacataaata tccccgtcac cagttcatta ccgcctgtta accacatggt gaagtaataa
      481 atgagtgcag cgaggatcac catgccgaag aaagaagaga ccgccgatac cacgttcagt
      541 acctgaccga tgtagccgct tacgcagaca atacacaggg cgagaaacat ccataaataa
      601 atcatagctc aacctcctgc cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa
      661 gacaatcctt aacctctggc aatttttgct aagcatcaat gaaaacagca tgttaaatgc
      721 aagactgttg tgtacggaaa aatatttact ttgcacgatt aataatcagt agctgaaagc
      781 agtcagcgag tgataaagta cagataccgc attcatgctc ctcgtggtta tgtcctgact
      841 agtctttaca ctctttacag gaaccattgt cgtacatgat ggcctaatca attgagtgta
      901 ccgctgtcga catggaatct tctgcgatac aacaattcgt atctacagaa ggtaactatg
      961 tttccacaat gcaaattttc ccgcgagttt ctgcatcctc gctactggct cacatggttt
     1021 gggcttggtg tactctggct ttgggtacag cttccttatc ctgttctctg ctttctcggc
     1081 acgcgtatcg gcgcaatggc gcgaccattc ctgaaacggc gtgaatctat cgcccgtaaa
     1141 aatctggaac tttgcttccc gcagcattct gcggaagaac gcgagaagat gattgccgaa
     1201 aacttttgtt cactcggcat ggcgctggta gaaaccggca tggcgtggtt ctggcccgac
     1261 agtcgcgtac gtaaatggtt tgatgttgaa gggctggata accttaaacg cgcacaaatg
     1321 caaaatcgcg gcgtaatggt tgtcggcgtc cattttatgt cgctggaact gggcggccgc
     1381 gtgatgggac tttgtcagcc aatgatggct acctatcgtc cacataataa tcagctgatg
     1441 gaatgggtgc agacccgtgg tcgcatgcgc tctaacaaag cgatgatcgg cagaaataat
     1501 ctgcgcggca ttgtcagtgc actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag
     1561 gattatggtc gtaaaggcag ctccttcgcg ccgttctttg cggtagaaaa tgtcgccaca
     1621 accaatggca cctatgttct ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg
     1681 gtaagaaaag cggattacag cggatatcgt ttgttcatca ccccagagat ggaaggctac
     1741 ccgacagatg aaaatcaagc cgctgcctat atgaacaaga ttatcgagaa agagatcatg
     1801 cgcgcaccgg agcagtacct ctggatccac cgtcgcttta aaacgcgccc ggtgggagaa
     1861 tcgtcgttat acatttaaca aactgctctg actctttacc tcctcacata agccataata
     1921 acctcatggt ttttatggct tttttcttaa ggttttttcc ttcgctgaac atctaattat
     1981 taccgtcaga aattaaactg tcgccgatat gagacacctg taagttacta aaagtaaata
     2041 agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt
     2101 tagctcagaa ttctggggtg atgcccacgg taaaatacag gacttttaag gtcgttttgc
     2161 ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc aggcgtaaca gcaattacgc
     2221 ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca tcgtttgggc
     2281 cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag gagccatcag
     2341 gctcctgttt gcatttctga ggtcggtgtt tattccgcgt tttcgtgaat gtgtttgctg
     2401 ctggcgggta gtaaaccgtc ggcacggaac atcgctttaa tcccacgaat cgcctgacga
     2461 atacggtcgc ggttttcaat cagtgcaaag cgcacatgcg tatcaccata gtcgccaaag
     2521 ccaatccccg gagagacaca gaccttcgct tcgttaagca atttcttggc aaattccagc
     2581 gagcccatgg cagcatacgg ttccggtatt ttcgcccaga catacatcga agccttcggc
     2641 atttcgacca tccagcccgc ttcatgcagc cctttaacca atacatcacg gcggcgtttg
     2701 tactgttcgg caatgtcgcg cacacactgt tgatcgccct cgagtgccgc aatcgccgcc
     2761 acctgcaacg gcgtaaaggt gccgtaatcg tgatagcttt taatacgtgc cagggcgctg
     2821 accagcgttt tgttgccaac cataaagccg atacgccagc ccgccatgtt gtagcttttc
     2881 gacagcgtaa agaactcgac cgccacatcg cgtgcacctg gcacctgcat gattgacggt
     2941 gctttccaac catcgtagac gatgtccgca taggccaggt cgtggaccac cagcacatcg
     3001 tagcgtttcg ccagcgccac aaccttttcg aagaactcca gttccacgca ttgcgcggtt
     3061 gggttcgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc acgaatggcg
     3121 cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg tacctgcgcc
     3181 ccggcaatca ccgcgccata aatatgaatg gggtagcttg gattcggcac cagcaccgtg
     3241 tcaccatgat ccagcgtcgc caacatcagg tgtgccagcc cctctttcga accaatggtg
     3301 acgatggctt ctgattccgg gtcgatttca acgtcgtagc gatcctgata ccagcgggaa
     3361 atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc
     3421 tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc
     3481 atactgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag ttcagcggta
     3541 atgttaaaaa catagggcgg gagacgatca atgcgcgtaa agcgacgttc agggcgagtg
     3601 tcagccataa tttcctcaga ttaacgttag cgcccggacc gtccgagcga cgctgccacg
     3661 atggtggctg ttttgaaaat agcctgatta atttctgtct gtccagcgtt ttagaacatt
     3721 tttattaatg acgttaaatg aggaactctg ctttaaaaac aggaaattta tctgcgctaa
     3781 caagaaagtt accactcatt ttattaacat aataatttca ataaatttac taaccagaag
     3841 tgcattatca agagatgcat tgctaataca ccatttatct atcaatcccc ttttaaaatt
     3901 gtggtttttc ctcaaaaacg gcctgctggt catcagcctt caggttacct atcatagagg
     3961 tttaatcctt attcagagtc acccgtgcac gaaatattca acatgctgct ggcggtcttc
     4021 gatcgggcag cgttaatgct tatctgcctg ttctttctca tccgtatccg cctgtttcgc
     4081 gaactgttgc acaagtcggc acactcaccg aaagagttgc tcgccgttac cgccattttc
     4141 tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg tagaaggctc gctggtgaac
     4201 gtacgtatta tcgcggtgat gtcaggcggg attctgtttg gcccgtgggt gggcatcatt
     4261 accggcgtga ttgcgggtat tcatcgttat ttaattgata tcggcggcgt gacggcgatc
     4321 ccctgcttta tcaccagcat tctggcgggt tgtatatcgg gctggatcaa cctgaaaatc
     4381 cccaaagcac agcgctggcg cgtcggtatt ctcggcggca tgttgtgtga aacgttgacc
     4441 atgattctgg tgattgtctg ggcaccaact accgcgttgg ggatcgatat cgtctctaaa
     4501 atcgggattc caatgatcct cggcagcgtc tgtatcggct ttattgtgct tctggtgcaa
     4561 agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcagg ccaagttggc gctggatatc
     4621 gccaacaaaa cgctacctct gtttcgccat gtcaatagcg agtcattacg caaggtctgc
     4681 gaaattatcc gcgatgacat tcacgccgat gcggtggcaa ttaccaatac cgatcatgtg
     4741 ctggcctatg ttggcgtggg tgaacataac tatcataatg gcgatgactt cattagcccg
     4801 actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga tgaagcccac
     4861 cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg cgttgtgacc
     4921 ggaacgctga aaatttacta ctgccacgcg catcagataa cctcgtcatt acaggaaatg
     4981 gcggttggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc cgagcagcta
     5041 cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa tccccatttc
     5101 ctgtttaacg ctttgaacgc tatttcatcg tcaatccgtc tgaatccgga taccgctcgc
     5161 cagctgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa agacgatgag
     5221 caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat tgagcaggcc
     5281 cgctttggcg acaagctgac ggttatctat gatattgatg aagaggtgaa ttgctgcatt
     5341 cccagcctgc tgatccagcc gttggtggag aatgccattg tccacggtat ccagccttgc
     5401 aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gcaatcgggt acgcattgcg
     5461 gtgcgggata ccgggcacgg catcgatcca aaggtgattg agcgggtcga agcgaatgaa
     5521 atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt attgtatggc
     5581 gaggggctgc atatccgccg cctggaacca ggaacggaaa ttgcgtttta cattcctaac
     5641 caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt gatattgtga
     5701 aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg ctaattaaag
     5761 agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt
     5821 tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg ctggatggtg
     5881 tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg ttcatcaccg
     5941 cgtggaaaga acatgcggta gaagcgtttg aacttgaggc gtttgactac attctcaaac
     6001 cgtatcagga gtcacgcatt accgggatgc tgcaaaaact ggaagcggcc tggcagcaac
     6061 agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc attaatctgg
     6121 tgaaagatga gcgaataatc gtcaccccca ttaacgatat ctattacgcc gaagcgcacg
     6181 agaaaatgac ctttgtttat acgcggcgtg aatcctacgt aatgccgatg aacattaccg
     6241 aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca
     6301 atctgaacaa aatccgcgag atcgaaccgt ggtttaataa cacctacatt ctgcgactga
     6361 aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt taaagaattc cgccagttaa
     6421 tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat caacaatttt
     6481 tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgg gtctggtttg
     6541 ccagccagcc tgcctcgttg ccggtgggca gtttatgtat tgattttccc cgtctggata
     6601 tcgtgctgcg cggcgaatac ggcaatctgc tggaagcaaa gcagcaacgt ttggtggaag
     6661 gagaaatgct gtttattccg gcgcgcgcgg ctaatttacc ggtcaacaac aaaccggtga
     6721 tgctgttaag cctggtgttc gctccgacct ggcttgggtt atcgttttac gatagccgca
     6781 ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa cgcggtgaag
     6841 gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag caaaatatca
     6901 ttcagccgct ggtgttaagt ttgctgcatc tttgccgtaa cgtggtgaat atgccgccgg
     6961 gcaattcgca gccgcgcggc aattttctct atcacagcat ttgtaactgg gttcaggata
     7021 attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc
     7081 atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg
     7141 tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcgatt catgaagtgg
     7201 cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg cgtcagtttg
     7261 gtctgacgcc gggagagtac agcgcccgct ttcagggcta acgtcagaag gttaattccg
     7321 tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt
     7381 ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc atcccttcgt
     7441 tagcacccag cgtcagcatg atcaccagtt cgacttcgcc catttctgac tgccagtcaa
     7501 ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga gatttggtgt
     7561 gcgggatcgc cacgccaaaa ccaacgccag tggtgacaat ctcttcccgc tgccagacat
     7621 cctcttccag ctcgaacgga tgttcagtgc gcccgttaac gccgaggtta ccgcacagga
     7681 actggatcgc ctgctcttta ttgctaaagg cctgatcaac gaagatattc tccagcgcca
     7741 gcagcgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca atttcctgcg
     7801 cgctgcgaca ttcacacgcc tgacgtgcca gttcccgaca cgcctcgcta tccagttgac
     7861 gaagctggct tttcaccgcc ggaatgcgcg ggctactcat actcagctca tccaggccca
     7921 gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa atgcctaccc
     7981 atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg aacgatggcg
     8041 taatcgggtt atacagcggc gatacgcgcg ggttattacg atcgaccgca taaagatact
     8101 gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag tgatcgatga
     8161 tgtagcacac cgacggcact tccaccatga tacccagcgt aatcgtctct gcatgacgca
     8221 ggccatcgcg cttaagctca acgatcgctt tttggatctc gcccttcacc cataagatct
     8281 gatcgaggct gtggaccatc gggatcatca actgggcgtt gccgaaactg gcggcgcgca
     8341 aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc
     8401 gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg cttttatcgc
     8461 ctccgatgtc cattgtgcgg aagataatcg gcttgtcgcc cgctgccagt agcacctgct
     8521 ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata tagagcattt
     8581 cggtacggaa cagcccgacg ccttccgcgc cgttggcaaa cgcgccaggc gcttccagag
     8641 cggtgccaat attcgccgcg atatcaatac gcttgttatc acgggaataa gccagctgcg
     8701 cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg acctgataat
     8761 aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg tcaagcactg
     8821 caggttgccc ggcataacgg gcaatcgcat ccagcggcag gccgctcagt accgggatcg
     8881 ccgaggcgcg ggccagaatc agggtatgcg aggtgcgccc ggttttctcc agaatcatgc
     8941 ccgcaagatt ttttaaatcc aggcttaaaa actggcttgg ggttaaatcc tcggccacca
     9001 gaatagtcgg tttttccagc accagattgt tgcgcggctt cagttccggc caggtgatat
     9061 gcagtaactg ttcgctgata tcgcgaatgt cgctaacacg ttcgcgcaga taatcactgg
     9121 cagaagcaga aagtttggcg caaacctgct ccatattgct gatgatcgcc gcccccagcc
     9181 cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca tcctgaatca
     9241 gcgacaaatg ggcgctgagg atagttttgc tttcgccgtc acgctcacgc aattgctggt
     9301 tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct tgcgcactgg
     9361 cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc
     9421 cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac
     9481 gcggcagcgg atgcgccgtc agttctgcct gcgtaggctg aacgctgtcg ataaagcgca
     9541 cctggatgta ctcttccagc acgcgccgcg cctgctcttc atcgctgccg ctaatgttca
     9601 ggctgcaact gtcattaaat agggtgccgg tgccaatcag cgccagcgag cttttggcat
     9661 cggcctttgc gttctggcga tggttaataa aagtgatttc gctttgccac tggctggact
     9721 gttctttaag ttcccacgcc ggacgagcat gtagaccgtt aggcaaagga cagagaaatt
     9781 gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc gtcagttgaa
     9841 caaccgtctc acgcgtaaga cgttgaataa gggcagataa aagttgctcc atctgcaaaa
     9901 tgtcgcggca atcggcaatc gacgcggcgc aatgtccgtg gcgggtggca ggccccatca
     9961 ccactgtggg tacgccagta ccggttaagt gcaccgctcc accgtccgtg ccgccgttac
    10021 tgaacatatc tgcctgcaac ggcacgccaa tttctgccgc cacggtttcg acccaggcgg
    10081 taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggc ccgttaccaa
    10141 tctggcgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg gtatcaagca
    10201 caatggcgac gtccggcgac accgcgcggg tggcagtttg cccgccgcgt aatcccacct
    10261 cttcgctgga acttgccacc agccacactt ccgcaggcag ttcggcgtcg tgcagctcac
    10321 gtagtaacgt caccagcaga tagcaaccga ggcggtcatc aaaggctttc cccatcactc
    10381 gctggtgggg gagaacctga aaagtggtat cgaacgtgac gcgatcgcct ggacgaattc
    10441 ccgcctgcat cacttcgtca tacgagcgcg cgccaatatc cacgcgcatg gtgctgacgt
    10501 cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca cgggtggtga
    10561 tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc agcacatcaa
    10621 ttgcgccttc gcgggagata ctgcgcacca taaatccaac ttcgtccata tgcgcacaga
    10681 tcatcacttt cggacctgtc gattcattga ggcggatcag caccgatccc agcccatcaa
    10741 atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc acttcctgtt
    10801 ccgaggaggc gatcgcatct gcctcgctca acgctttcaa tagcgataaa tccattatgc
    10861 ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc cttgcggggt
    10921 gaccagcaca acatcctcga tgcgcacacc cccttgccct ggcaaataaa tccccggctc
    10981 cacggtcagt aacatgcctg gctgtagcgt cgtggtatcc cgcggtgaaa agcgcggatc
    11041 ttcgtgtact tcaatgccaa tagcatgacc ggtgttgtga ccaaaatagt cgccaaatcc
    11101 tgcctcggta atcacccgac gcgcggcgtc gtcaacctgc tggcagcgca cgccggggcg
    11161 aattgcagag attgctgcga gctgtgcctg caaaacaatc tgatagacgt taaacagcgg
    11221 atgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca tatcagagca
    11281 gtagccctga tacagcgcac cgaaatcgag agtgacaaac tcgcccgctg caacaatctt
    11341 gtcgctggct ttgccgtgcg gcagcgcccc acgccagcca ctggcgacaa tggtgtcaaa
    11401 agcggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg
    11461 ctcgctcatc cccgcctgaa taaagcggcg aatatgctct gcaccgcgat cggcaatccc
    11521 acaggcaagg cggattttct ccacctcctc tggcgttttg atttgccgca gcacatccgg
    11581 cgtggcgcta accagtttcg cattgagttc agactgccag cggtgcgcgg tttcccaact
    11641 cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgcctggc
    11701 gatggttgta agcgtgtgcg tcgcgtcaag caaatgcagc tggtaacctt gcgtgcgggc
    11761 ttctacatcc gcgtaatagc gcgagtccac cagaatgtgc gcactttcac ggctaatcag
    11821 cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag
    11881 aagcaccgca tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga gtaatgtcat
    11941 ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa catcatcaga
    12001 cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc cgccatatag
    12061 acgcccaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact
    12121 gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata cgacggaatg
    12181 gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactgatccc cataaaggca
    12241 aggaacatcg cggtcttacc ctgcggataa agttgtgcgc tgaacaggcg tttgccggtt
    12301 aaacgacggt cgataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata
    12361 gaacgtgcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac aaaacctgcg
    12421 gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac
    12481 atcagcgcgc ccttctcacc tgcggcagtc agcacggtac ggataccgcc gttgatccag
    12541 ccaccaaagg gggtgatgac gtagtacatc gccagcatga caaaaatagc ggaaagaatc
    12601 ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc
    12661 atccatttca caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac
    12721 ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc
    12781 ggctgggtag acatcaagcc gccgataaaa ccagccggga acgccagttt gccaccgata
    12841 gagttcgcca caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag
    12901 gactgcgaca accaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta
    12961 ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcaac
    13021 tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct
    13081 gacatcacat gctgcgggag ttcaccccag aagctggttt tagaggcctg cggattctta
    13141 actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc
    13201 ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct
    13261 ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgtgt
    13321 gattcgaaac gttcgttatc ttccggggta actgccacgg agtggatgat gatggtcgct
    13381 tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg ggtttcaatt
    13441 ttcacttcat aaccggcttc taccgccgct tcttccagcg cctgagcggc cataaaagtg
    13501 tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc
    13561 tcgttcagtg atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag
    13621 acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc
    13681 ctggtgagga ggacacaaag aaaagttgaa ttaactgtat gatttaaaag attatcggga
    13741 gagttacctc ccgatataac aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa
    13801 tgtgcaccgg aaccgagaag acccggattg tcatggacga tgagatacac cggaatatca
    13861 tggacatatt ctttaaagcg ccctttatct tcaaatgcgg cacggaaacc agaggctttg
    13921 aagaactcaa ggaagcgcgg cacgataccg cccgcaataa acacgccgcc aaatgtcccg
    13981 agattgagcg ccagattgcc gccaaaacgg cccataatga cgcaaaacag cgacaacgca
    14041 cggcggcaat cggtgcagct gtcagccagc gcgcgttcgg taatatcttt tggcttgaga
    14101 ttttctggca ggcggttgtc ggctttaaca attgcgcgat acaaattcac aagtcctggg
    14161 ccagaaagca cacgttccgc cgaaacatga ccaatttccg cacgcaatat ttcgaggata
    14221 atggcctctt cttcactatt cggcgcaaaa tcaacgtgac cgccttcgcc tggcaagctt
    14281 acccaacgct tatcgacatg gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa
    14341 accgcaatag gcttaccttc tatcggctct gcgccaccaa actgaatcag atgctctttt
    14401 ttcagcatcg gaatcgccat cgatacagcg gtaaaatcgt taataatttc cagatggcta
    14461 aaaccgagat tctttttcat ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc
    14521 acccagtcac cggtaattgg gcaagcgatg gcaatacagc cgtctttcac ctcgacctta
    14581 tgttcttcaa gataaacgcg aatgaccgct tcgaggcttg ggtaatcaag ccctgaatag
    14641 gtcttagcct gcgagatttc accactggca atatcacaca gagcaagacg tgcgttggtg
    14701 ccgcccacat caccgactaa tgcatacttt gtcattcttc aactgctccg ctaaagtcaa
    14761 aataattctt tctcacactg taaatacctg ggggcataac aacaacgccg gaaaggcagg
    14821 ctccctgtaa atatcgatct gggtcacaca attactttat cgtttcagca ccaattgcag
    14881 cgatgccttt ttgcaagctg ggcaaactaa gtatctgacc ccgcataagg aatagaacat
    14941 gctccatccg cgagccagaa ccatgttgtt attatcgctc cccgctgtgg caattgggat
    15001 tgcgtccagt cttattctga ttgtggtgat gaaaatcgcc tcggtattac agaatttgct
    15061 ctggcaacga ctgccgggaa ctctggggat agcccaggat tcacctttct ggatcatcgg
    15121 tgtattaacg ctaacgggta ttgcggtggg gttggttatc cgtttcagcc agggtcatgc
    15181 cggaccagac cccgccagtg aaccgctgat cggcgcaccg gttccgccct ctgcgctacc
    15241 aggacttatc gtagcattaa ttctcggtct tgctggcggc gtcagcctgg ggccggaaca
    15301 tccgatcatg accgtcaata tcgcccttgc cgtcgccatt ggcgctcgtc tgttaccgcg
    15361 cgtcaaccga atggagtgga ctattttagc ctctgccgga accatcggcg cactgtttgg
    15421 cacacctgtt gcggcggcgt tgatattttc gcaaacctta aatggcagta gtgaagttcc
    15481 gctatgggat cgtctctttg cgccgttaat ggcggcagca gctggtgcac ttactaccgg
    15541 attatttttc catcctcatt tttcactgcc cattgcgcat tacggacaga tggaaatgac
    15601 cgatattctc agcggtgcaa ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt
    15661 atggtgctta ccacgattgc acgcgatgat gcatcaaatg aaaaatccgg tgctcgtgct
    15721 gggtattggc ggatttattc tcggtattct gggggttatt ggtgggccgg tttcgctgtt
    15781 taaagggctg gatgagatgc agcagatggt ggcaaatcag gctttcagca ccagcgatta
    15841 ctttttgctg gcggtgatta aacttaccgc cctggtcgtt gctgccgcca gtggctttcg
    15901 cggtggacga attttcccgg cagtgttcgt cggcgtggca ttagggttga tgctgcatga
    15961 gcacgtcccc gccgttccag cggcaataac cgtttcctgc gctattctcg gcatcgtgct
    16021 ggtggtaaca cgcgatggct ggttaagcct ttttatggcg gcagtcgttg tacccaatac
    16081 cacattgcta ccgctgctct gtatcgtcat gcttccggca tggctgttat tagcaggtaa
    16141 gccgatgatg atggtcaatc gtccgaagca acagccaccc aacgataacg tttagcaaaa
    16201 aatgctctcc tttatgattt aagagttatg gcgatgatac ggggctttac ctccccgtaa
    16261 tattgcgtta acaggccgct gacggcttcg atcgtgaagg agaataacaa tgttcaggtc
    16321 actgtttctg gcggccgccc tgatggcatt taccccgctt gcggcaaacg caggcgaaat
    16381 caccctactg ccatcaatca aattacaaat tggcgatcgc gatcattacg gtaattactg
    16441 ggacggtggt cactggcgag atcgtgatta ctggcatcgc aattatgagt ggcgcaaaaa
    16501 ccgctggtgg cgtcatgata atggctacca ccgtggctgg gataagcgta aagcgtatga
    16561 gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca
    16621 tgggcaccgc cattaaacac atttgccaat ggagcacaag gcctgatgcg acgctagtgc
    16681 gtcttatcag gcctacaaac cggctcttta acgcgtaggg cggataaggc gtttacgccg
    16741 catccgccaa tagtgccaga tgcgacgctc attcaactac aatcccagcg ccgtccccac
    16801 caacaaccag atattcagcg ccacgaccag cactacaatc acccagcctg tctgttttac
    16861 gcgtttgctg ttcaccagat cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg
    16921 aaccagcgcc agggcgatac caaaacttaa cagcacctga ctcataacca gaatccgtgt
    16981 cggatctaat cccatcagaa tgacaataaa tgatggcaac atggtgactg tacgacgcac
    17041 ccacagcggg atatggaagc gaatgaaccc ctgcatcacc acctgccctg ccagtgtccc
    17101 caccaccgtt gaggacagtc cggcagcaac cagacttaac ccaaagaccg ttgccgcagc
    17161 atggcttaac agcggttgca gcgtcaaata agcctcatca agatcggcaa cacctgtatg
    17221 accagaaaag tggaacgccg ccgcagctgt agccatcatc gccagattga caaaaccggc
    17281 aatagtcatg gcgatagcca catcccattt ggtggcggaa taacgttgtt gacgcgaacc
    17341 gccatgtaaa tgctgagtga gcgaagagtg caaataaatc acatgcggca taatcgtcgc
    17401 ccctaacacg cctgcagcca ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat
    17461 tcctttaccc agctgcgcca ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc
    17521 ggcaacaaac aacaataacc cgccaatcac tttctccagc ggtttttgcc cgcgacgttg
    17581 cagcattaaa atcaggaaag tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac
    17641 accaagaatg agtttaaaac cgatcgccgc accaataaat tccgccaggt cggttgccat
    17701 cgcaataatt tctgcctgaa cccaatagaa ccacactacg ggacgcggat agtgatcacg
    17761 aatctgctcc gccagatttt taccggtggc aatccccagt ttggcagaga ggatctgaat
    17821 cagcatcgcc atcaggttgg cccaaacgac aacccacagt agctgatagc cgaagctggc
    17881 acccgcctga atattggtcg caaagttacc gggatcgata taaccaatcg ccgcaatgaa
    17941 cgcaggtccc attaatgcga gcctcatctt gcgcgccgcc cgtccgctgc tactctcaac
    18001 gcgatagttc gtcatcttgt gcctctaaaa catagccttt gctatgtttc atgctatgcc
    18061 aaacgagaat gattatcaaa ttcatttaaa tgggttgtgg tgatttctct gatagaccag
    18121 gattatgact acgaaaagat tgatgacttg aatgttatgg ttatgtttaa tgttagcaca
    18181 tttacataac tttcagcttc catacacaac atagcagaaa tgtatgacag ctcactattt
    18241 ttgaagcttg tcacaggacg tcattatagt gtgtgtcaga tctcgttttc cttaaccatg
    18301 ttacatagaa tgtgcacgga aatttaacct gcctcatatt tggagcaaat atggaccgcg
    18361 tccttcattt tgtactggca cttgccgttg ttgcgattct cgcactgctg gtaagcagcg
    18421 accgcaaaaa aattcgtatc cgttatgtta ttcaactgct tgttatcgaa gtgttactgg
    18481 cgtggttctt cctgaactcc gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt
    18541 tcgaaaaact gctcggattt gccaacgaag ggactaactt cgtctttggt agcatgaatg
    18601 atcaaggcct ggcattcttc ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac
    18661 tgatcggtat tctccagcac attcgcgtgt tgccggtaat tatccgcgca attggtttcc
    18721 tgctctccaa agtcaacggt atgggcaaac tggaatcctt taacgccgtc agctccctga
    18781 ttctgggtca gtctgaaaac tttattgcct ataaagacat cctcggcaaa atctcccgta
    18841 atcgtatgta caccatggca gcaacggcga tgtccaccgt gtcgatgtcc atcgttggtg
    18901 catacatgac catgctggag ccgaaatatg tcgttgcggc gctggtactg aacatgttca
    18961 gcacctttat cgtgctgtcg ctgatcaacc cttaccgtgt tgatgccagt gaagaaaaca
    19021 ttcagatgtc caacctgcac gaaggtcaga gcttcttcga aatgctgggt gaatacattc
    19081 tggcaggttt caaagttgcc attatcgttg ccgcaatgct gatcggcttt atcgccctga
    19141 tcgccgcgct gaacgcactg tttgccaccg tgactggctg gtttggctac agcatctcct
    19201 tccagggcat cctgggctac atcttctatc cgattgcatg ggtgatgggt gttccttcca
    19261 gtgaagcact gcaagtgggc agtatcatgg cgaccaaact ggtttccaac gagttcgttg
    19321 cgatgatgga tctgcagaaa attgcttcca cgctctctcc gcgtgctgaa ggcatcatct
    19381 ctgtgttcct ggtttccttc gctaacttct cttcaatcgg gattatcgca ggtgcagtta
    19441 agggcctgaa tgaagagcaa ggtaacgtgg tttctcgctt cggtctgaaa ctggtttacg
    19501 gctctaccct ggtgagtgtg ctgtctgcgt caatcgcagc actggtgctg taagaccata
    19561 cataaaaaag ccggggataa ttccccggct tttttacgcc tgttaatcag ctaatggctg
    19621 cgggcgacca atcaaatacc cttgcagata ttgcaccccg agcttatgca atagcgcctg
    19681 ttgctgttgc gtctcgacaa actccgcgac cacactcaat gacttcgctt tcgccagatc
    19741 ggtaattgat ctaacaatca tcgcatccag cgtgttcgtg acgatatctt tcacaaagac
    19801 gccatcaatt ttgatgatat cagcctgcaa acgctttaac cgttcgtagt tggcatagcc
    19861 ggtgccaaag tcatcaatcg caatccggaa accaaactta tgcagctgct cgatgttgta
    19921 catgctgctt tctgcgttag aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg
    19981 cggggagatg tgataacgct taaatagacg aataatccgc ccggcaatat tcttttgcag
    20041 cagcgtgagc ggcattaaat taactgaaaa gcgcgggcct tttttgtcgc aagggtgtgt
    20101 tgccagccac ttcaacaagg attccagcac ttgcaaatca aaacgcgcgc taaggttaaa
    20161 ctgagcaata aggggcagaa acttatccgg ggtcataatg ccgccgtcat atttcagtcg
    20221 cgcgaggatc tcatcataac cttcaccttc tttgttgcga attggctggg cgtagagcag
    20281 caaatcaccc tgatctaacg ccgtgcgaat ggtattcagc aatagcacct gtttagtggt
    20341 ctgcccggaa accatctcct ctctgctatc cagcgccagc acatgatgat gtgcgcagga
    20401 ttgctccgcc agccagctta actgccccaa caagggttgc agggtttcct gatttccatc
    20461 aaaacgcccc caggcagcac catagcccat atccagcccg gtattgttcc agtgaatttg
    20521 ccgactattc aggatgttaa ccatatgctg gagtcgccct tccgtttccg gcccgctcag
    20581 cactaacagc agttcactac ccggcaattg atacaacttt tcgttttcct gcatcaacgg
    20641 cagcagcgta cggtagattg agcggataca gtgaacgcgc atcattaagc cgtaatgacg
    20701 actcataaac tcaagattat caatgcgcag gcagcaaaaa ctcttgccca cctcttgctc
    20761 cggtgcttgt tccaacgcac gaaagttggg taggagcgtt aacggatcag tcagcgcctg
    20821 caaatgccag cggcgattaa gccattcact gcgatgataa atgcgcacca tatagagcag
    20881 gcaaacgcta aaggaaatca gcaccgcaag aataaatgcc agcgaatatt ctgtttccac
    20941 cccttgcaaa aagttctggt tgtaattcag caggcaaagc gttgaaacag cccaggtgag
    21001 atttaaaaac ggatagcgaa gcttaccgac cccgagggta aagatgatga agaaaacggg
    21061 taccaggtaa ccggcaataa agtcgttttc ataaggtgtg cacaacagaa gtagcagcac
    21121 gcttaacgct gccagccagc ttaaggtaaa tgcgcgtttc tctttgccca acgacggagc
    21181 gatatccctg cgccacaata tctgcgcaaa gtggggactt acaatcatgc gagtgagata
    21241 gtagaaaagc atgttgtaaa tcagcacagc ggtgaaaagg cttagcaaat caacgaccgt
    21301 gaaaatggca tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct
    21361 ccccacaaga tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag
    21421 acgttgccag acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg
    21481 caatattgcg caggcacaca acacgacaaa cgtctggcta ccgagcatga ccgcagtctg
    21541 taaaaaagag agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac
    21601 gacaccgcgc cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc
    21661 cagaaagata taactggaat ggataatggc aagtggcgaa ataaaacggg aaagctgaat
    21721 aagtaccacg gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg
    21781 ctccacaaac atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa
    21841 gtttattcgc atcgcgaagc aaacaggggc catcctgttg ccgatttttc atccaatgat
    21901 gctttcacca ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag
    21961 agacaaaaaa cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg
    22021 aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat
    22081 gcgtttacgt accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg
    22141 ctgacctctt gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa
    22201 gcttgtcgag ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt
    22261 gcagcgtgta gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc
    22321 gcattcagta gttattcatg tcacggtttc ctgtaaagtg gtgctataaa atgaactact
    22381 aatagaccca catacattca gggaattgtt atgttcaagg agaggatgac gccagatgaa
    22441 cttgccagac tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc
    22501 tggacgacgt caccaaaacc aggcgtccag ggtggcaaag cccgactggt tcacgtcaat
    22561 gaacaggttc gtgaatatat tcgtaatgct gaacgtccag aaggtcaggg agaagcgcct
    22621 gccctttctg gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg
    22681 ccagttgaac aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta
    22741 caacgcttag ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac
    22801 cgaagaactg gctgaatgcc tcggtgtggc taaacaaaca gttaaccgtt ggatcagaga
    22861 aaaaggctgg aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct
    22921 ggtcgatacg caagtttgcg agtttattca gaacacgcca gccttccata acacaccgat
    22981 gcttctggaa gccgaagaac cccttgccga atacgcaccg gggattcgca ctcctgctta
    23041 tcggcaaatc ataagcgcaa tagacaacat gactcacagt gagcaggaaa aagttgcgca
    23101 atttttgtca cgcgaaggaa ttcgtaattt ccttgctcgt ctcgacatag atgaatcagc
    23161 ttgacatcct ccacgccctg aatgacgagg atccctgcta cgttcaggct gtcgcctgaa
    23221 tcatttcggt gggttcctgc ttcaacgagt ggcctgactg caccatctct ccacaggcaa
    23281 gaacggcgtg ccccgccgct aaaatgttac gagcgccgtt tacatcggcg ctcgctgtat
    23341 atccacatac ctggcatctg aatttacttt gtgacaggcg attttctttc gctgtatgac
    23401 cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc tgaccgcctc
    23461 gccagagctg cttgtactca agctggcggc gcatttcata ccagccctga tccagtatcg
    23521 aacggtttaa acctgatttt gcccggacat tgcgccccgg ctggctgacc gtacccgctg
    23581 ctgactttga catgttgctg accttcaaat cctcaatgac tatcattgcg tggtttttgc
    23641 tgacggtcgt tgtgactttg tgaaggtagt ctctgcggat atttgcggta caggaatgca
    23701 gtcgctgtat tttgcgtttc tgcttctgcc agttattgct gaatttgacc ttgcggctta
    23761 actgtcgctg aagtctcgcc agcgtcttct ggtttttctg aaaactgttt acaggctcaa
    23821 agactgtgcc atctgacagc gtggcgagtt tagccacgcc agcatccagt ccgaccattg
    23881 atgctgaagg gtgaaccgga gttgatactt cactttctgt ctgaatactg atgtaccact
    23941 taccgcagga ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggctgttcc
    24001 ggtagcgcat ccagccaagt ttcggcagaa aaatacggct gttttcctga tcgagcttaa
    24061 cgccctgctg gtagcggaat gcatcattct gtccccgctt tttgaatcgg ggaaaagcag
    24121 cccgcttccg gaagaagttt ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg
    24181 gctgtgaggg cgaatcttta agccattgcg tttcagtggc gtttttccac tcaaccagcc
    24241 aggaagccat tttcccgtaa gggatgtgtt tattaccggc ctcatgattc tcattctgac
    24301 gtgccagagc acgattgaaa acgaaacgac atgcacctgc gaagcgcctc atttcacgct
    24361 cctgttgacc accgggtctt aactggaatt taaatgcctg tagtcgtttc acaggccaaa
    24421 tgatactttg gtctgtgaaa aaagaaaccg atatccggcg tggaaggcat tgtgttttcc
    24481 tgatgcatgt tcacctggtc tttgtcccaa atactgacgc caggtttttg accatgacgc
    24541 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct gattttgaag ctgaactggt
    24601 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactatcctc ccaaactggc
    24661 gatattcagt ctggtaaaca gcctcaaagg catatcgaat aggttactgc gacgagatcg
    24721 accagatatt gcagtcaggt attactacaa aggcgttatg tggagtcctg gctattttgc
    24781 cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac aacagcaaac
    24841 acctggttag gtggaaaacc gcgccttata tccccgccct gaaggacggg gttttacggc
    24901 gcaccggata aataaaaaac ggcaggggta tttctcctgc cgtttatctt ttatgcgcta
    24961 attactgctg gttttcacgt tccgcaataa aagacagcgc tttgttgata cgctcgatac
    25021 tgcgggtctt accgattgcg tgaacggtaa catccagtgc tggagactgc cccgcaccgg
    25081 ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg
    25141 tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg gccagtttgt
    25201 cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc gccgcatcgg
    25261 catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg
    25321 tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat
    25381 cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc ggcggcagcg
    25441 cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt
    25501 tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag atttcctgat
    25561 cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt
    25621 catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt ttgccgtcat
    25681 cgccgttgat catagaaacg tgcgcgtaaa ccggtaccgg cgctttcagg gctttaagaa
    25741 tgttgatctg gcgtggcgtg ttgttgatat ggtcttcgcc acggataacg tgggtgattt
    25801 ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca tcggtacggc
    25861 ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg atctgatcgt
    25921 caaaaacaac agaaccttcc tgcgggttag caaaacgtac gacacacggc tcatcatcag
    25981 cgtgatgctc atgactgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca
    26041 tttgctcttc acgcagcgct tccaggcgct ctttagagca atagcattta taagctgtgc
    26101 cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg gtctggaagt
    26161 acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag
    26221 cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt
    26281 gattacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg tgcagatagc
    26341 ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag
    26401 atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct
    26461 gattcctcaa tgtagttcgg gttgcaacct tacgcattat tgttttcgtt gagaaattgc
    26521 gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt
    26581 agaaaatgcg ttgactcatt ttgaactctc cctataatgc gactccacac agcggggggg
    26641 attagctcag ctgggagagc acctccctta c
//
LOCUS       DAZUGK010000043        26562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZUGK010000043 DAZUGK010000000
VERSION     DAZUGK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.043"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(44..925)
                     /gene="yddG"
                     /locus_tag="ACHY5W_001608"
     CDS             complement(44..925)
                     /gene="yddG"
                     /locus_tag="ACHY5W_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFS4446521.1"
                     /translation="MTRQKATLIGLIAIILWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVIIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1157..4204
                     /gene="fdnG"
                     /locus_tag="ACHY5W_001609"
     CDS             1157..4204
                     /gene="fdnG"
                     /locus_tag="ACHY5W_001609"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1742..1744,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS4446522.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRIMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4217..5101
                     /gene="fdnH"
                     /locus_tag="ACHY5W_001610"
     CDS             4217..5101
                     /gene="fdnH"
                     /locus_tag="ACHY5W_001610"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFS4446523.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHSLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5094..5747
                     /gene="fdnI"
                     /locus_tag="ACHY5W_001611"
     CDS             5094..5747
                     /gene="fdnI"
                     /locus_tag="ACHY5W_001611"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFS4446524.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5975..6259)
                     /locus_tag="ACHY5W_001612"
     CDS             complement(5975..6259)
                     /locus_tag="ACHY5W_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS4446525.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLRGFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVAQ"
     gene            complement(6405..7412)
                     /gene="adhP"
                     /locus_tag="ACHY5W_001613"
     CDS             complement(6405..7412)
                     /gene="adhP"
                     /locus_tag="ACHY5W_001613"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFS4446526.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGASLKLGDRASVAWFYEGCGHCEYCNSGNETLC
                     RSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIRP
                     GQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDAA
                     KIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDGI
                     EVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDFR
                     R"
     gene            complement(7546..9243)
                     /gene="maeA"
                     /locus_tag="ACHY5W_001614"
     CDS             complement(7546..9243)
                     /gene="maeA"
                     /locus_tag="ACHY5W_001614"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS4446527.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9399..9536)
                     /gene="sra"
                     /locus_tag="ACHY5W_001615"
     CDS             complement(9399..9536)
                     /gene="sra"
                     /locus_tag="ACHY5W_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFS4446528.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9638..9853)
                     /gene="bdm"
                     /locus_tag="ACHY5W_001616"
     CDS             complement(9638..9853)
                     /gene="bdm"
                     /locus_tag="ACHY5W_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFS4446529.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10199..10630
                     /gene="osmC"
                     /locus_tag="ACHY5W_001617"
     CDS             10199..10630
                     /gene="osmC"
                     /locus_tag="ACHY5W_001617"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFS4446530.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10665..11435)
                     /locus_tag="ACHY5W_001618"
     CDS             complement(10665..11435)
                     /locus_tag="ACHY5W_001618"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFS4446531.1"
                     /translation="MNLQGKTALVTGSTSGIGLGIAQTLANAGAQVILNGFGDVDAAI
                     NAVSRMGKTPGYHEADLSDESQIINMVAYARREFGGVDILVNNAGIQYVSPVESFPVE
                     KWNAIIAINLSSVFHTTRLALPEMRTRNWGRIINIASVHGLVASKDKSAYVAAKHGVV
                     GLTKTIALETAQTGITCNALCPGWVLTPLVQQQIDKRIAQGEDAESARDALLAEKQPS
                     REFVTPQQLGELALFLCSDAAAQVRGVAWNMDGGWVAQ"
     gene            complement(11447..12838)
                     /locus_tag="ACHY5W_001619"
     CDS             complement(11447..12838)
                     /locus_tag="ACHY5W_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFS4446532.1"
                     /translation="MSVLIALAALGLLMLAAYRGYSVILFAPIAALGAVLVTDPGAVG
                     PAFTGLFMEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAINILGRRHAIPVI
                     VLVCALLTYGGVSLFVVAFAVYPFAAELFRQSNIPKRLIPATVALGAFSFTMDALPGT
                     PQIQNIIPTSFFGTNAWAAPWLGLIGSLFIIAVGLIYLERQRRKAQAKNEGYGTELLN
                     EPETPDNINLPNPLIAISPLILVGLLNLLFTWWIPVWYGASHQLELPGLAAPITTDVA
                     KITAIWAVEAALIAGILLVVIFGYRNIHGRLAEGSRTAVGGAILAAMNTASEYGFGAV
                     IAALPGFLVLSKALAAIPDPLLNEAISITTLAGITGSASGGMSIALAAMSDTFIAAAH
                     AANIPLEVFHRVASMASGGMDTLPHNGAVITLLAITGLSHRQAYGGIFAITIIKSLAV
                     LFIIGVYYTTGIV"
     gene            complement(12884..13807)
                     /locus_tag="ACHY5W_001620"
     CDS             complement(12884..13807)
                     /locus_tag="ACHY5W_001620"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HFS4446533.1"
                     /translation="MQTEQFAVLGAGLMGVGIATHFARFGHDIWLYDTEQSRIAEIHV
                     VAGGILDELISCEQFTAPEKENVLKRLHGTTSLADIADSQLLIEAIPERLELKHALYT
                     ELETLISPEAIVASNTSGLPPDDLAAKMRYPQRLLIAHFWHPPHFIPLVEIIPGSVTE
                     PKHLHWLQHLLVSLQLEAVLLDRAAPGFVGNRLQFALLREALHIVKSGIASAEVVDQV
                     MRASLGRRYAMVGPLEAADMTGLSTVQDICRHLLPELATGSDMMSLVAEKVERGDIGV
                     RSGQGFYCWDESRKQYIQQSREHQLRFALKP"
     gene            complement(13797..15002)
                     /locus_tag="ACHY5W_001621"
     CDS             complement(13797..15002)
                     /locus_tag="ACHY5W_001621"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS4446534.1"
                     /translation="MQDVVIVAATRTPIGCFHGALAPLSAVDLGAATIRSLLEKTALA
                     AEQIDEVIFGQVLTAGSGQNPARQSALKAGLSHNTPAVTINLVCGSGLKAVHQAVQAI
                     RCGDAQIIIAGGQESMSNAPYFIDGARAGLRLGHTSMKDSVVHDGLWDAFNDYHMGIT
                     AENLAQKYAISRERQDTFALRSQQKAAAAMEAGRFADEITPVSVPQGKKPPRWVNTDE
                     QPRSGTQLEKLAQLKPAFLPGAGSVTAGNASSINDGAAAVMLMRASKAAELGLPVLAR
                     VAGYAVSGVDPAFMGIGPVAATRECLKRCGWTLEDIDLIEANEAFAAQALAVGDELNW
                     DCDKVNVNGGAIALGHPIGASGCRILVTLIYEMARRNAQKGLATLCVGGGQGVALTLE
                     RTTGEHHAN"
     gene            complement(15012..15668)
                     /locus_tag="ACHY5W_001622"
     CDS             complement(15012..15668)
                     /locus_tag="ACHY5W_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HFS4446535.1"
                     /translation="MLTREQMAMRVARELRDGYYVNLGIGIPTLVANYIPDGMDVMLQ
                     SENGLLGMGAFPTEDELDADMINAGKQTVTARKGAAIFDSAQSFAMIRGGHVDLTVLG
                     AFEVDVNGNIASWMIPGKMVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPDCTLP
                     LTGAGCIKRVLTDLALLEIADGAFILRERAPGISVAEIIAKTAGKLFVPEHVPEMQFN
                     "
     gene            complement(15671..16378)
                     /locus_tag="ACHY5W_001623"
     CDS             complement(15671..16378)
                     /locus_tag="ACHY5W_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFS4446536.1"
                     /translation="MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRH
                     RGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASYVGENALFEKQVLNGELEAILTP
                     QGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
                     KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQ
                     GQFEKRIEQRTLSRKGA"
     gene            16520..17410
                     /locus_tag="ACHY5W_001624"
     CDS             16520..17410
                     /locus_tag="ACHY5W_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4446537.1"
                     /translation="MKMSIKQLRAFLAVAHTLNFAHASERLNMSQPALSLTIKGLEDA
                     LGGALLLRTTRKVTLTQEGETLLSMARPLLADWDNTEEAMRQRFTLRRGKVSVAAMPS
                     FAANVLPPVLKTFRDCYSGINVTVHDVVNEQVIEMVSEGRVEMGIAFEPDYCGHVHFT
                     PLGVDRFVAIVPADSRLANRQRVSWRDLLTLDFITLQRPSAVRLMLEEQLAQSGTQLE
                     VALESHQLVTVGRLVANGLGGSAVPALCRRQMSELGAVCLELDNPVIERRVGVLRSSH
                     HKLSTAAQALMETLKKTYDA"
     gene            complement(17447..18373)
                     /locus_tag="ACHY5W_001625"
     CDS             complement(17447..18373)
                     /locus_tag="ACHY5W_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4446538.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLAIREDGREIRCWRAL"
     gene            complement(18366..19352)
                     /locus_tag="ACHY5W_001626"
     CDS             complement(18366..19352)
                     /locus_tag="ACHY5W_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4446539.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(19349..20245)
                     /gene="ddpC"
                     /locus_tag="ACHY5W_001627"
     CDS             complement(19349..20245)
                     /gene="ddpC"
                     /locus_tag="ACHY5W_001627"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFS4446540.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(20242..21264)
                     /locus_tag="ACHY5W_001628"
     CDS             complement(20242..21264)
                     /locus_tag="ACHY5W_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4446541.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTGTVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            complement(21266..22816)
                     /locus_tag="ACHY5W_001629"
     CDS             complement(21266..22816)
                     /locus_tag="ACHY5W_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4446542.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKINVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTILTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(22830..23411)
                     /gene="ddpX"
                     /locus_tag="ACHY5W_001630"
     CDS             complement(22830..23411)
                     /gene="ddpX"
                     /locus_tag="ACHY5W_001630"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFS4446543.1"
                     /translation="MSDTTELVDLAVTFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(23669..26068)
                     /gene="dosP"
                     /locus_tag="ACHY5W_001631"
                     /gene_synonym="pdeO"
     CDS             complement(23669..26068)
                     /gene="dosP"
                     /locus_tag="ACHY5W_001631"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFS4446544.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(26093..26562)
                     /locus_tag="ACHY5W_001632"
                     /pseudo
     CDS             complement(26093..26562)
                     /locus_tag="ACHY5W_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         6421 a         6901 c         6896 g         6344 t
ORIGIN      
        1 aatttatctg attaactgcc gggccgtaga cccggcagtt attttaacca cgacgtgtcg
       61 ccagccagca gagcagggaa ccgccgcaga ccattagcgc gccttgccag aatgagaacg
      121 acagcggggc gctgagcagc acggctgcaa gcgctgagga aagtacgggc gtaaaatacg
      181 aacctaccgc cataatggtg acgttgccat gcaatatacc gacattccat gcagcataag
      241 caaatcctaa ggtaaatgcc gcagagatga gtttaattat gacgggcgtg ctaaatacca
      301 tttctggttg tggcgtgaga aaatagtaaa cccacagact tgctcccgtt agcaggacaa
      361 aaacggtaat tccgttaaat ccgcgggcgt atttattcgt tactgtgcaa taagcggccc
      421 agataaacgc accaataaac gccaggaaat aactcaatgg actggtggtg atattattga
      481 tgatttcatc ataatgtaac ccattgtcac cgcctaacac ccagcagacg ccgacgaggg
      541 ctaataataa tcctggtaca atcaaccagt tggttttctg accattaaac agaatggcaa
      601 agagaattgt caggctgggc catagatagt tcaccatacc cacttcaatc gcctgatgac
      661 gggtcgccgc ataccctaag gaaagcgcca gacagatttc atagctgacg aataacagac
      721 tcccggcgag caaatagcct ttcgggattt gccgaatacg cggaaatcca accgtgaaga
      781 ttaacagcag cccgcttaat gaatagatag cagctgcgcc gccgaccggg ccgagcccct
      841 cactgacacc gcgaatcaat cctaccatcg tgctccacag gatgatcgct atcagcccta
      901 tgagcgttgc tttttgtcgt gtcatgctcg ctgttttgtc tcttttgccg ttaaaaatta
      961 agctgaattt tatagcattt ttttaactgg cctgtcaggc agtggtgcgt ttttctaccg
     1021 ctattgaggt aggtcaattt gcgcaggcgg attattttgt ggcaaacacg tgttcttttt
     1081 gatttcgcgc aaaaagattc agaattttac tgttagtttc ctcgcgcagt aatacccctg
     1141 aaaaaagagg aaagcaatgg acgtcagtcg cagacaattt tttaaaatct gcgcgggcgg
     1201 tatggctgga acaacagtag cggcattggg ctttgccccg aagcaagcac tggctcaggc
     1261 gcgaaactac aaattattac gcgctaaaga gatccgtaac acctgcacat actgttccgt
     1321 aggttgcggg ctattgatgt atagcctggg tgatggcgcg aaaaacgcca gagaagcgat
     1381 ttatcacatt gaaggtgacc cggatcatcc ggtaagccgt ggtgcgctgt gcccgaaagg
     1441 ggccggtttg ctggattacg tcaacagcga aaaccgtctg cgctacccgg aatatcgtgc
     1501 gccaggttct gacaaatggc agcgcattag ctgggaagaa gcattctccc gtattgcgaa
     1561 gctgatgaaa gctgaccgtg acgctaactt tattgaaaag aacgagcagg gcgtaacggt
     1621 aaaccgttgg ctttctaccg gtatgctgtg tgcttccggt gccagcaacg aaaccgggat
     1681 gctgacccag aaatttgccc gctccctcgg gatgctggcg gtagacaacc aggcgcgcgt
     1741 ctgacacgga ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa
     1801 ccactgggtg gatatcaaaa acgctaacgt cgtaatggta atgggcggta acgctgctga
     1861 agcgcatccc gtcggtttcc gctgggcgat ggaagcgaaa aacaacaacg atgcaacctt
     1921 gatcgttgtc gatccccgtt ttacgcgtac cgcttctgtg gcggatattt acgcacctat
     1981 tcgttccggt acggacatta cgttcctgtc tggcgttttg cgctacctga tcgaaaacga
     2041 caaaatcaac gccgaatacg ttaagcatta caccaacgcc agcctgctgg tgcgtgatga
     2101 ttttgctttc gaagacggtc tgttcagcgg ctacgacgct gaaaaacggc aatacgataa
     2161 atcgtcctgg aactatcagt tcgatgaaaa cggctatgcg aaacgcgatg aaacactgac
     2221 tcatccgcgc tgtgtgtgga acctgctgaa agcgcacgtt tcccgctaca cgccggacgt
     2281 tgttgaaaac atctgtggta cgccaaaagc cgacttcctg aaagtgtgtg aagtgctggc
     2341 ctccaccagc gcaccggatc gcacaaccac cttcctgtac gcgctgggct ggacgcagca
     2401 caccgtgggt gcgcagaaca tccgtattat ggcgatgatc cagttgctgc tcggtaacat
     2461 gggtatggcc ggtggcggcg tgaatgcatt gcgtggtcac tccaacattc agggtctgac
     2521 tgacttaggt ctgctctcta ccagcctgcc aggttatctg acgctgccgt cagaaaaaca
     2581 ggttgatttg cagtcgtatc tggaagcgaa cacgccgaaa gcgacgctgg ctggtcaggt
     2641 gaactactgg agcaactatc cgaagttctt cgttagcctg atgaaatctt tctatggcga
     2701 tgccgcgcag aaagagaaca actggggcta tgactggctg ccgaagtggg accagaccta
     2761 cgacgtcatc aagtatttca acatgatgga tgaaggcaaa gtcaccggtt atttctgcca
     2821 gggctttaac ccggttgcgt ccttcccgga caaaaacaaa gtggtgagct gcctgagcaa
     2881 gctgaagtac atggtggtta tcgatccgct ggtgactgaa acctcaacct tctggcagaa
     2941 ccacggcgag tcgaacgatg tcgatccggc gtctattcag actgaagtat tccgtctgcc
     3001 ttcgacctgc tttgctgaag aagatggttc tattgctaac tccggtcgct ggctgcagtg
     3061 gcactggaaa ggtcaggatg cgccgggcga agcgcgtaac gacggtgaaa ttctggcggg
     3121 tatctaccat cacctgcgcg agctgtacca ggccgaaggt ggtaaaggcg tagaaccgct
     3181 gatgaagatg agctggaact acaagcagcc gcacgaaccg caatctgacg aagtagctaa
     3241 agagaacaac ggctatgcgc tggaagatct ctatgatgct aatggcgtgc tgattgcgaa
     3301 gaaaggtcag ttgctgagta gctttgcgca tctgcgtgat gacggtacaa ccgcatcttc
     3361 ttgctggatc tacaccggta gctggacaga gcagggcaac cagatggcta accgcgataa
     3421 ctccgacccg tccggtctgg ggaatacgct gggatgggct tgggcgtggc cgctcaaccg
     3481 tcgcgtgctg tacaaccgtg cttcggcgga tatcaacggt aaaccgtggg atccgaaacg
     3541 gatgctgatc cagtggaacg gcagcaagtg gacgggtaac gatattcctg acttcggcaa
     3601 tgccgcaccg ggtacgccaa ccgggccgtt tatcatgcag ccggaaggga tgggacgcct
     3661 gtttgccatc aacaaaatgg cggaaggtcc gttcccggaa cactacgagc cgattgaaac
     3721 gccgctgggc actaacccgc tgcatccgaa cgtggtgtct aacccggttg ttcgtctgta
     3781 tgaacaagac gcactgcgga tgggtaaaaa agagcagttc ccgtatgtgg gtacgaccta
     3841 tcgtctgacc gagcacttcc acacctggac caagcacgca ttgctcaacg caattgctca
     3901 gccggaacag tttgtggaaa tcagcgaaac gctggcggcg gcgaaaggca ttaataatgg
     3961 cgatcgtgtc actgtctcaa gcaagcgtgg ctttatccgc gcggtggctg tggtaacgcg
     4021 tcgtctgaaa ccgctgaatg taaatggtca gcaggttgaa acggtgggta ttccaatcca
     4081 ctggggcttt gagggtgtcg cgcgtaaagg ttatatcgct aacactctga cgccgaatgt
     4141 cggtgatgca aactcgcaaa cgccggaata taaagcgttc ttagtcaaca tcgagaaggc
     4201 gtaagggggc gaacagatgg ctatggaaac gcaggacatt atcaaaaggt ccgcaactaa
     4261 ctccatcacg ccgccttctc aggtgcgtga ttacaaagca gaagtcgcaa aacttatcga
     4321 cgtttccacc tgtatcggct gtaaagcctg tcaggtggcg tgttcggagt ggaacgacat
     4381 ccgtgatgaa gtggggcact gcgtcggggt ttacgataac cccgccgatc tgagcgccaa
     4441 gtcctggacg gtgatgcgct ttagcgaaac cgaacagaac ggcaagctgg agtggctgat
     4501 ccgtaaagat ggctgtatgc actgtgaaga tccgggctgc ctgaaggcgt gcccgtctgc
     4561 cggtgcaatc attcagtacg ctaacgggat tgtcgatttc cagtcggaaa actgcatcgg
     4621 ctgtggttac tgcattgccg ggtgtccgtt taatattccg cgcctcaaca aagaggataa
     4681 ccgggtatat aaatgcacgc tctgcgtcga tcgcgtcagc gtcggccagg aaccggcttg
     4741 tgtgaaaacc tgtccgaccg gggctatcca cttcggaacc aagaaggaga tgctggagct
     4801 ggcggaacag cgcgtggcga aactgaaagc gcgtggttac gaacatgctg gcgtctacaa
     4861 cccggaaggg gtcggtggta cgcacgttat gtacgtgctg catcacgccg atcagccgga
     4921 gctgtatcac agtctgccga aagatccgaa gatcgacacc tcggtaagcc tgtggaaagg
     4981 cgcgttgaaa ccgctggcag cggctggctt tattgccact tttgccgggt tgattttcca
     5041 ctacatcggt attggcccga ataaggaagt ggacgatgac gaggaggatc atcatgagta
     5101 agtcgaaaat gattgtgcgc accaaattta ttgatcgcgc ctgtcactgg accgtggtga
     5161 tttgcttctt cctggtggcg ctgtccggga tttcgttctt cttcccgacg ctgcaatggc
     5221 tgacgcaaac cttcggtacg ccgcagatgg gacgcatttt gcacccattc ttcggcattg
     5281 cgattttcgt cgcactgatg tttatgtttg tgcgttttgt gcatcacaac atcccggata
     5341 agaaagatat tccgtggctg ttaaacattg tcgaagtatt aaaaggcaat gagcataaag
     5401 tggcggatgt cggtaagtac aacgccgggc aaaagatgat gttctggtcg atcatgagca
     5461 tgattttcgt gctgctggtg accggggtga ttatctggcg tccgtacttt gcgcagtact
     5521 tcccgatgca ggttgttcgc tacagcctgc tgatccacgc ggctgcgggt atcatcctga
     5581 tccacgccat cctgatccat atgtatatgg cattctgggt gaaaggatcg attaaaggga
     5641 tgatcgaagg gaaggtgagc cgtcgctggg cgaagaaaca ccatccgcgc tggtatcgtg
     5701 aaatcgagaa ggcagaagcg aaaaaagaga gtgaagaagg gatataatct cttttgaact
     5761 ttaagctgaa aatggcgctg taaaaggcgc cattttcata ttgtagacaa cgtaggcttt
     5821 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaccgttca aatccaatag
     5881 attgcagtga acgtgtaggc ctgataagcg tagcgcatca ggcaatgttg cgtttgtcat
     5941 cagtttcaaa tggcgctgta aaaggcgcca ttttttattg cgcaaccaga cgacgtaatc
     6001 gcgacacatc caccgttttt tcagcctctg ccagactcca ggcgttttgc agattcagcc
     6061 acatttgcgg cgaactgccg atcaccacgg aaagtttaat tgccatttct ggcgtcaaag
     6121 ctgcttttcc ggtcagcaat cggcttgccg ttgagggagc aatttccatt gctctggcaa
     6181 acccgcgcag gctgacatta agttcgtcca gtgattcctg aataatgtcc cccgggcggg
     6241 gatgattggc cattttcatt agtgtcttta ctctcagtag cgttccttct ctgggaacac
     6301 tatcattatt gttccttcct acgcaacaat aatccattta gccatttcat gttggagagt
     6361 gtgcgatgct gcaacccgaa catggcagtc gcagcaaagg cctcttagcg gcggaaatca
     6421 atcaccatac ggccacggat tttgccttct tccatctcgg taaagatggt gttgatgtcc
     6481 gctaacggac gcagggcgac tttcggcacc actttacctt cggcggcaaa ctggaaggct
     6541 tcagttaaat cctggcgcgt gccgaccagc gaaccgacca cttcaatacc atccagcaca
     6601 agacgtggga tatccaggct catagactcc ggcggcagac cgactgccac aacacgccca
     6661 cctgcacgga cagcatcaac tgccgagtta aacgcagctt tagctactgc tgttaccacc
     6721 gcagcgtgag cgccaccagt tttctcctgt acaattttgg cggcgtcttc ggtgcgtgag
     6781 ttaatcgcta aatcagcgcc catttcggtt gccagtttta actgctcatc attgacatca
     6841 atggcgatca ctttcgcgtt aaagacattc ttcgcgtatt gcagggcgag gttacccaga
     6901 ccgccaagac cgtagatagc aatccactga ccgggacgaa tttttgacag tttaacggct
     6961 ttgtaggtgg tgacccccgc acaggtaatg ctgctggccg ccgccgagtc cagaccatct
     7021 ggcactttta ccgcgtaatc ggcgaccacg atgcactctt ccgccatccc gccatcaacg
     7081 ctgtatccgg catttttaac tgaacggcag agcgtttcgt taccactgtt acagtattcg
     7141 caatgaccgc atccttcgta gaaccacgcc acgctggcac gatcgcctag ttttaatgag
     7201 gcacctggac ccacttctgc caccacaccg atgccttcat ggcccagaat tacgccggtt
     7261 ttgtcaccaa aatcgccatt cttaacatga agatcggtat gacatacacc acaacactcc
     7321 attttcagca gggcttcgcc atgtttcagt gagcgcagtg ttttatccgt aacgtcaaca
     7381 tgatgatcct tcgtaacaac tgcagccttc atagttcctc cttttcggat gatgttctgc
     7441 atagcaggtg aggcaaatga gatttattcg ccactaccca gtatggatga gatctgaaaa
     7501 agggagaggg aaattgcccg gtagccttca ctaccgggcg caggcttaga tggaggtacg
     7561 gcggtagtcg cggtattcgg cttgccagaa attatcgtca atggcctgtt gcagggcttc
     7621 ggcagaggtt ttcaccgcca cgccttgctg ctgcgccatt ttgccaaccg caaacgcaat
     7681 tgcgcgggag actttctgaa tatctttcag ttccggcagt accatacctt cgccgttcag
     7741 cactaatggt gaatactgcg ccagcgtttc acttgccgac atcagcatct catcggtgat
     7801 acgtgacgcg ccggaagcaa taacacccag gccgatgcct gggaaaataa aggcgttgtt
     7861 acactgggcg atagggtaga ttttatcttt ccataccact ggattaaacg ggctgcccgt
     7921 ggcgaccagc gcgttacctt cggtccaggc gataatgtcc tgcggtgtgg cttccacgcg
     7981 tgacgtcggg ttagacagcg gcatcacgat cggacgcgga cagtgtttat gcatctcacg
     8041 gatgatctct tccgtaaaca gcccggtctg tcctgagacg ccaatcagaa tatctggttt
     8101 tacattgcgc accacatcca gcagtgacag cacatcgctg tcggtatccc agtcactgag
     8161 gttttcgcgc ttctgcacca gtttggtctg gaaaggcagc aggttcggca tcttgtcagt
     8221 cagcaggcca aagcgatcga ccataaagac tttctgcctc gctgcttcct cgcttaatcc
     8281 ttcacgctgg gtctgggcga tgatcatttc ggcaatgccg catcccgctg aacctgcgcc
     8341 aaggaagacg atttttttct cgctcagttg accacctgcc gcgcggcttg ctgcgatcag
     8401 tgtgccgact gttaccgccg cagtgccctg aatgtcatcg ttaaaagaac aaatttcatt
     8461 gcgatagcgg ttaagtaacg gcatcgcatt tttttgagca aagtcttcaa actgcaacag
     8521 cacgtctggc cagcgttgtt tcacagcctg gataaattca tcaacgaatt catagtattc
     8581 gtcgtcagtg atacgcggat tacgccagcc catatacagc ggatcgttaa gcagctgttg
     8641 gttattcgtc ccgacatcca gcaccaccgg aagggtatac gccgggctga tgccgccaca
     8701 ggcggtatag agcgacagtt taccgatcgg aatgcccatc ccgccgatgc cctggtcacc
     8761 aagccccaga atacgttcac cgtcagtcac cacaatcact ttaatattat ggtttggtac
     8821 gttttgcaga atatcgtcca tattgtgccg gttctggtaa gagataaaca cgccgcgtga
     8881 acggcggtag atctcagaaa aacgctcaca ggctgcgccg acggttgggg tataaataac
     8941 aggcatcatc tcatcaagat gattgtttac cagacggtag aagagggttt cgttagtgtc
     9001 ctggatgtta cgcaggtaga tgtgtttgtc gatttcggtt ttgaatccct gatactggat
     9061 ccatgctcgt tccgcttgtt cttcgatggt ttcgaccact tccggcagta accccagcag
     9121 gttgaagtta cggcgttctt ccatgctgaa ggcactgcct ttattcaaca acggaaattc
     9181 cagcagtaca gggccagcgt aagggatata aagcgaacgc tgtttttttg tttttggttc
     9241 catgtcactc actctttttt gaatatccat ccctgggggc ttttatcgtc tttgctttac
     9301 cgccagggcg tcggccccaa gtataaagca gataaaaaca aaaacaccat tgcgcaggca
     9361 atggtgttta atcgtcattg aggactaatg gttatgaatt acttttcagc ggggcgtttt
     9421 ctgccggttg ggttatttac cacgctggat ttgtcacctt cggtaactat tttgcgctgg
     9481 ttagaaattt tatggtccag tccaagaata tgacgtgcct gacggttcga tttcatttta
     9541 actcctcaat cctgttgcta gttttaagga caacatcgcc gtagcgaaga aacacgtgct
     9601 aaacccctaa attaggttgc cgatcaagca tagcacctta aagcgtaggg tgctggccac
     9661 tgaccacata attgatcgtt tgttggtaga tatcactgag gatgtcgtta tcagaagctt
     9721 ccacccattt ggtcagctcc atgagaatgt catcttcagt gacaacaccg tgctctgccc
     9781 gaaggccttg ctcaatggca ttaaccagag cgggttctgc tgttgaattt tctgcctgat
     9841 aataagtaaa catagtgatt ctccgtgtct gtgtatttat ggtgtctgct acggatcgca
     9901 gatttataaa gcatattcag catggcaaat atttgccgct tcgttgttaa gattagtcct
     9961 ggttgatgat ttttatattt taacaccatg atattcatag ggattgtgat tgggactatt
    10021 cgattaatat tgatgcaata tcttttgggt tatatattcc ccgttatcaa ttgtgggaat
    10081 ttaatttaag tgcagaagta atattttctg ccggatttta ttcggaatat cctgcttatc
    10141 ctcgtgctgt ttctcacgta gtctataatt tcctttttaa gcccacagga gagcaacaat
    10201 gacaatccat aagaaaggtc aggcacactg ggaaggcgat atcaaacgcg ggaagggaac
    10261 agtatccacc gagagtggcg tgctgaacca acagccgtat ggatttaaca cgcgttttga
    10321 aggcgaaaaa ggaaccaacc ctgaggaact gattggcgca gcgcatgccg catgtttctc
    10381 aatggcgctt tcattaatgc tgggggaagc gggattcacg ccaacatcga ttgataccac
    10441 cgccgatgtg tcgctggata aagtggatgc cggttttgcg attacgaaaa tcgccctgaa
    10501 gagtgaagtt gcggtgccgg gtattgatgc ctctaccttt gacggcatca tccagaaagc
    10561 aaaagcagga tgcccggtct ctcaggtact gaaagcggaa attacgctgg attaccagtt
    10621 gaaatcgtaa agcattgccg gatgacgcgt catccggcga acacttactg cgctacccaa
    10681 ccgccatcca tattccacgc caccccacgc acttgtgctg ccgcatccga acagagaaac
    10741 agcgccagtt cacccagttg ttgcggagtc acaaattcac gagaaggctg tttttctgcc
    10801 agtaaggcgt cacgcgcgct ttccgcatct tcgccttgcg caatgcgctt atcaatctgc
    10861 tgttgtacca gcggcgtcag cacccagcca gggcagagcg cgttgcaggt aatacccgtc
    10921 tgcgcagttt ccagagcaat ggtctttgtg agtcccacca cgccatgttt tgccgcaaca
    10981 taagctgatt tgtctttcga cgctaccagc ccatgcacag aggcaatgtt aatgatccga
    11041 ccccagttac gagtgcgcat ttctggcagt gccagacgcg tggtgtgaaa gacagaagag
    11101 agattgatag cgataatggc gttccatttt tctacaggga aagattcaac cggggagaca
    11161 tactggatcc cggcgttatt caccagaatg tccacaccgc caaattcccg ccgtgcgtag
    11221 gccaccatgt tgataatttg cgattcatcg ctgaggtctg cctcatgata gccaggcgtt
    11281 ttacccatac gagaaaccgc attgattgcc gcgtcaacat caccgaagcc gttgaggatc
    11341 acttgcgcgc cagcgtttgc cagtgtctga gcaataccga gtccgatacc gctggtggac
    11401 cccgtcacca gcgcagtttt accttgtaaa ttcatgatga ttttccttag acaatgccgg
    11461 tggtgtagta cacgccgatg ataaacagca cggccagact tttaatgatc gtaattgcga
    11521 agatgccgcc gtaagcctga cggtgactca gcccagtaat tgccagcagg gtgatcacag
    11581 cgccgttatg gggcaatgta tccatgccgc cactggccat agatgccacg cggtgaaaaa
    11641 cttccagtgg aatattggca gcgtgcgcag cggcaataaa agtgtcggac atagccgcaa
    11701 gagcaatact catcccgccg gacgctgaac cggtaattcc ggcaagtgtg gtgatgctga
    11761 tggcttcgtt gagtaatgga tcaggaatgg cggccagcgc ctttgagagc accagaaaac
    11821 cgggtagggc ggcgataacc gcaccgaagc catattctga ggcggtgttc atcgcagcca
    11881 gaattgcgcc gcccaccgct gtacggcttc cttctgccag tcttccatga atattgcgat
    11941 atccaaaaat cactaccagc aaaattccgg caatgagcgc agcttcgacg gcccagattg
    12001 cggtgatttt ggcgacatct gtggtaatcg gggcggcaag tcccggcaac tccagttggt
    12061 ggctcgcacc gtaccaaaca gggatccacc aggtaaacaa caagttcaac agccccacca
    12121 gaatcaatgg tgaaatcgca atgagtgggt tcggtaaatt gatgttatct ggtgtttcag
    12181 gttcattaag taactctgtg ccgtaacctt cattttttgc ctgcgcttta cgacgctgcc
    12241 gctccagata gataagcccg acggcaatga taaacagtga accaataagc cccagccacg
    12301 gcgcggccca ggcgttggtg ccaaagaaac tggtaggaat aatgttctga atttgtggcg
    12361 tgccgggcag agcgtccata gtaaaagaga atgcccccag ggccaccgtt gccggtataa
    12421 gtcgttttgg gatgttactc tggcggaaca gttcagccgc gaacggataa acggcaaatg
    12481 ccaccacaaa cagcgacact ccgccatagg tgagtaatgc acataccagc acaatcaccg
    12541 gaatcgcatg ccgccggccg agaatgttga ttgcagcagc gacaatcgaa cgagaaaatc
    12601 ctgaaagttc aatcagtttt ccgaacacgg ccccgagcag aaacaccggg aagtacaatt
    12661 tcacgaagcc gaccattttt tccatgaaca ggccggtaaa tgctgggcca acggcccccg
    12721 gatcggtgac aagaacagcg cctaatgctg cgatgggggc aaaaaggata acgctgtatc
    12781 cgcgataggc tgccagcatt agcagcccaa gcgcggcaag ggcgattaat acactcatcg
    12841 ggactcctca atatcgttgt tatagtgggt acagcgagag aagttatggt ttaagggcga
    12901 agcgaagttg atgttcccga ctctgctgga tgtactgctt ccgcgattca tcccagcagt
    12961 aaaatccctg accgctacgt acgccgatgt ctccacgttc cactttttcg gcaactaatg
    13021 acatcatgtc gctgccagtc gctagttcag ggagcagatg gcgacaaata tcctgcaccg
    13081 tcgaaagtcc ggtcatgtcg gcggcctcca gcgggccgac catggcataa cgtcgcccaa
    13141 gcgaggcgcg catcacctga tccaccactt ctgcactggc gatgccgctt tttacgatat
    13201 gcagggcttc acgcaacagt gcaaattgca gacggttgcc gacaaatcct ggcgcagcgc
    13261 gatccagcaa cactgcttcc agttgcagtg acacaagcaa gtgttgcagc cagtgcaaat
    13321 gctttggctc ggtaacgctg ccggggataa tctccacaag tggaatgaag tgcggcggat
    13381 gccagaaatg ggcgatcagc agtcgttgcg ggtagcgcat tttcgcggcc agatcatcgg
    13441 gcggcagacc gctggtattg ctggcgacaa tggcctcagg agaaataagc gtttcaagct
    13501 cggtatataa tgcgtgtttt agttcaagtc gctccgggat cgcctcaatc agtagttgcg
    13561 agtcggcgat gtctgcaaga gaagtcgtgc cgtgcagacg tttaagcaca ttctcttttt
    13621 ccggggcagt aaactgttcg cagctaatca gctcatcaag aattccgcct gcaactacgt
    13681 ggatttctgc aatccggctc tgctcggtat cgtacagcca gatatcatga ccaaagcggg
    13741 cgaaatgagt ggcaatcccc acgcccatca atccggcgcc cagtaccgca aattgttcag
    13801 tttgcatggt gttctcctgt ggttctttcc agtgtcagcg ccacgccctg accgccaccg
    13861 acgcacagcg tcgccagccc tttttgcgca ttacgtcgcg ccatttcata aatgagagtg
    13921 accagaatgc ggcaaccgga agcgccaata ggatgcccaa gtgcgattgc gccgccgtta
    13981 acattcactt tgtcgcaatc ccagttcagt tcgtcgccaa cggccagcgc ctgcgctgcg
    14041 aaagcctcgt tggcttcaat aagatcaata tcttccagcg tccatccgca acgcttaaga
    14101 cattcgcggg tagcggcgac cggaccaatg cccataaagg caggatcgac gccagaaacg
    14161 gcataacctg ccacccgagc cagcactggg aggcccagtt cagcagcttt gctggcgcgc
    14221 attaacatga ctgcggcggc accatcatta atggaagagg catttcctgc ggtgacgctt
    14281 cccgcccccg gaagaaaggc cggtttcagt tgcgctagtt tttccagttg cgtcccggaa
    14341 cgtggttgtt cgtcggtatt tacccagcgt ggtggttttt taccttgtgg tacgctcacc
    14401 ggagtaattt cgtctgcaaa gcgcccggct tccattgcgg cagcggcttt ttgctgagaa
    14461 cgtagcgcga atgtgtcctg tcgctcgcga ctgatggcat atttttgcgc caggttttct
    14521 gcagtaatgc ccatgtggta atcattgaaa gcatcccata gtccgtcatg aaccacgctg
    14581 tctttcatgc tggtatgccc gaggcgtaaa cctgcccgcg cgccatcaat aaaatagggc
    14641 gcgttgctca tgctttcctg gccacctgcg ataattatct gtgcatcacc gcaacggatc
    14701 gcctgcactg cctggtgtac ggctttcaga ccggaaccac ataccaggtt gatggtaacg
    14761 gcgggtgtat tgtggcttaa cccggctttg agcgccgatt gtcgtgccgg gttttgtccg
    14821 cttccggcgg tcaggacctg accaaaaatc acttcgtcga tctgttctgc tgcaagtgcg
    14881 gttttctcca gcaacgaacg gatggtggca gcaccgagat caaccgcaga aagtggggcg
    14941 agagcgccat gaaaacagcc aatcggtgtg cgcgtagcgg caacaataac aacgtcctgc
    15001 atggtgattc ctcagttgaa ttgcatttcc gggacatgtt ccggcacaaa caacttaccg
    15061 gcggttttgg cgatgatttc cgcaacgctg attcccggcg cgcgctcgcg cagaatgaaa
    15121 gctccgtcag caatttccag cagagcaagg tcggttaaga cgcgtttgat gcatccggcg
    15181 ccggtcaacg gaagggtaca gtcaggaagc agtttggatt cgccggattt ggaggcgtgg
    15241 gtcattacca caataatatt ctcggcaccg gcaaccagat ccatggcacc gcccatgcct
    15301 ttaaccatct tgccggggat catccatgag gcaatgttgc cattcacatc cacttcaaat
    15361 gcgccaagta cagtcagatc cacatgaccg ccacggatca tggcgaatga ttgcgccgaa
    15421 tcgaaaattg ccgcgccttt ccgcgcggta acggtttgtt taccggcatt aatcatgtcg
    15481 gcgtccagtt cgtcttccgt aggaaatgcg cccattccca gcaaaccgtt ttctgactgc
    15541 aacatcacat ccatcccgtc aggaatgtag tttgccacca gggtcgggat accaatgccg
    15601 agattgacgt aatagccatc gcgcaattcg cgggcgactc gcatcgccat ttgttcacgg
    15661 gttaacatgg tcaggctcct ttgcggctaa gggtgcgttg ttcaatgcgt ttttcaaatt
    15721 gcccctgaat gacgcgatta acaaaaatcc ccggggtatg gatagcggca ggatcaagtt
    15781 caccaggtgg cactatttct tccacttcca ccacggtgat ttttccggca gccgccatca
    15841 gtgggttgaa gttttgcgcg gtatgacgat aaatcacgtt gccgtaccag tcggctttcc
    15901 agcctttgac cagggcaaaa tcaccggtga tagcctgttc catgatgtac gggcgaccgt
    15961 tgaattcgcg gacttctttc ccttcggcaa cgggagtacc gtaaccggtg gcagtaaaga
    16021 acgccgggat acccgcgccg cctgcgcgca gttgttctgc cagggttcct tgtggtgtga
    16081 gaatcgcctc cagttcgccg ttaagcacct gtttttcaaa caaagcattc tcgccaacat
    16141 aagaagcgac gactttacgg atctgtcggc tttccagtag tacacccagt ccaaaaccat
    16201 ctacgccaca gttattggaa accaccgtta atccggttac tccacggtgg cgaacttcag
    16261 caatcagatt ctccgggatg ccgcacaggc caaaacctcc tgccagaatc gtcatctcat
    16321 tggtaagtcc ttcaagtgcc tcgtgatacg tagcgacccg tttatccaac ccagccatac
    16381 agttgccctc cagttgtcct tttcaacatc attaagggca aaaaatgatt tgttaatttt
    16441 aaatttgtga ctcattcatt tgtattattt atcaatataa tgttaattaa atgtattcag
    16501 taggttaacg gagtgccaga tgaaaatgag catcaaacag ttacgcgcgt ttttagcggt
    16561 agctcacact ctgaactttg cccatgccag tgaacggctg aatatgtcgc agcctgcgct
    16621 aagtcttacc attaaaggac tggaagatgc cttagggggc gctttactgc tgcgtactac
    16681 gcgaaaagtg acattaaccc aggaggggga aacgctattg tcgatggcac ggcctttgct
    16741 ggctgactgg gataacactg aggaggccat gcgtcagcga ttcacgctca ggcggggaaa
    16801 agtctcggtg gcagcgatgc cgtcatttgc agccaacgtt ctgcctccgg tgctgaaaac
    16861 cttccgcgat tgttattcag ggattaatgt caccgtgcac gatgtggtga atgagcaggt
    16921 gatcgaaatg gtgagcgaag ggcgtgtgga gatgggtatt gcctttgaac ctgactattg
    16981 cggccatgtg cattttaccc cactgggcgt ggatcggttt gtcgctattg ttccagccga
    17041 ttcacgactg gcaaatcggc aacgcgtgag ttggcgggat ttgctgacgc tcgattttat
    17101 caccctccag cgaccgtcag ccgtcaggtt gatgctggag gagcagttgg cacaaagtgg
    17161 aacgcaactg gaagtggcgc tggaaagcca tcaactggtt acggttgggc ggctggtggc
    17221 aaacggtttg ggaggaagcg ctgtgcctgc gctgtgccgc aggcagatga gcgaactggg
    17281 cgcagtgtgt ctggaactgg ataatccagt gattgagcga cgagtaggcg tactgcgttc
    17341 gtcacaccat aagctttcta ctgcggcgca ggcgttgatg gaaacgttaa aaaaaaccta
    17401 tgacgcataa cgtttccgca gacatgcaat agccggatgc cgcgtcctac agcgcccgcc
    17461 agcaacggat ctcacgtccg tcctccctta ttgctaatga ttgccggact tcgcatccgc
    17521 tggttgcaag ggggcaacgt tcgcggaaaa aacagccttg cggcaacgtg cggtttcccg
    17581 gcagatccgt tttacgtaat gcccattctt cctccagtgg tttatcaatg gcggggaggg
    17641 aatccagcaa taatcgggtg tatggatgtg ccggagccgt tagcacctgt tgcgcttccc
    17701 ctaattccac aatctgcccg agatacatca ccgccacccg atcgctcata tgacgtatca
    17761 ccgagacatt gtgtgaaatc agcacataag tcagcccgtg attttcctgt agcgttacca
    17821 gtaaattgag gatctgcgcc tgcacggaga tatccagcgc agaggttggc tcatcaagca
    17881 caatcacgtc aggctgcgaa gagagtgctc tggcaatggc gatgcgttgc cgctgcccgc
    17941 cggagaacgc atgaggcagg cggtcgagat actccggacg aatacccacc tgcacagcca
    18001 gctcctctgc caacgctcgc cgctgttgtt cactactacg cttagctatc cagagcggtt
    18061 ctgtgatgat gcgccacacc ggtaagcgcg gattgagcga agagagcggg tcctgaaaca
    18121 ccatctgcat aatgcgttgt gagccagaac ggatgtactg cccgtggctc ggttgcagca
    18181 tacccattaa aagctgtgcg agggtgcttt tgccgcagcc tgactcgccg acgatcccta
    18241 aggtttcacc acggcggatc tgtaaatcaa taccattaat ggcatgaaca tgttccgtag
    18301 ttttaccaag ccagttttta cgggccggga aattgatatg gacgtcgcgt aacgttaata
    18361 acgtgtcaga cacttatgac ctcctgctgc ggataccaac aggcgcagcg ctggttgttg
    18421 tcaccacacg ctgttagcgc cgggacgttt tcacactgtg cacctgccgc atagcaacga
    18481 tcgcgaaaag cgcagccgtc aggcaaatgg gtgaggtttg gtaccgtccc tggaatggcg
    18541 ggtaatggct ggcgtggtac tccatgttct ggtgcgcatt gcagcaaacc aatggtatac
    18601 ggatgccggg gatgatggat aacgtctgcc gtcacgccgc tttcaattac gcttccggca
    18661 tacatcacgt aaacgctatc gcacagttgc gacaccacgg ccatatcatg gctgatgaac
    18721 agtaccgcag ttccactggc gcgagcttta tgtttaagca aacgcagtac ctgcaactgt
    18781 accgtgacgt ccagcgccgt agtcggttcg tcggcaataa tcaattgcgg ctcgcaggaa
    18841 aatgccagcg caatcataac ccgctggcgc ataccacctg aaagctcaaa cggatagcgc
    18901 gacataactt ccacggcatc cgggatttgc atctcttcca gcagggcaat cgctttagct
    18961 ctggcttccc gacgacttat tggttgatga tgtcggatta cgtccatcat ctgaagacct
    19021 attcgacgtg tcggattgag ggcggtcatc ggttcctgaa agatcatcgc cactcgtgcg
    19081 ccgcgccact gacgaagctg tttttcccga gcgttaagaa catcttctcc cagcagggaa
    19141 atctgccccc gatgtacgca ataactgccc gtcggtagca gacgcataat cagcattgcg
    19201 gtgactgatt tacctgagcc ggattctccc accagaccga caatttcacc gcggttaatc
    19261 tgcaaggaca cattgttgag cgcgtgaaca tcaccgttaa aaccggggaa actcaaatgc
    19321 agttgttgaa tgtccagaac gggttgggtc atgactgctt tcctcctgct ttcgggtcaa
    19381 gcagatcgcg aataccatca ccaaagagat taaacccgac ggcggtaagc aaaatcgctg
    19441 cccccggaaa tgcgcaatac caccactgat cgagtacata gttgcgacca tttgctacca
    19501 tcgcgcccca ttcagcactc ggttgttgag cacccaggcc aataaatccc aacgtggcgg
    19561 ccattaaaat cgcgctaccg atatccagcg atgcctgcac gatcagcggc ggcagggagt
    19621 tacgtaaaat atgccagctg atcagatgcc aacgagacgc gccaaaggtt ttcgccgcct
    19681 gaacataggt atactggcgt acaactaatg cttgcccccg cgccaggcgc acataaaagg
    19741 gaattcgcac aatagcaatc gccagcatgg cgttaaataa actcggcccg agagcggcag
    19801 ccagtgccat tgtcagtacc aacgaaggaa tcgacagcat aatgtccatg atgcgcatga
    19861 tgatggcgtc tgcgcgtccg ccaagcacac cggatagaca tccgagtagc gaaccaatca
    19921 tacccgcaat ggcaaccacg actaatccgg cgagaattga ttgctgactg ccgaccagta
    19981 cgcggctgaa cagatcgcgt cccacttcat cggtgccaaa ccagtgcgcc gcagaaggcg
    20041 gcaaaaggcg ggcggttaaa tcaatggcgt tgggatcatg cgtcgcaagc cacggtgaaa
    20101 aaatcatcat caatagcatt aatacaatga tgactgcgcc ggtcacggtg agcggactgc
    20161 ctttcagcat ccataccagt tttgcaccgt taaatcgcgt ttgttttgct gggcgtacgg
    20221 cggacgtttc ctcgcttagc atcattcatc acctccacgt ccgatacgcg gatcaatcca
    20281 caaatagagc aaatccacca ccaggttgac cagcacataa gcaaatgaaa ccacgacggc
    20341 aaagcccatc actgccggga agtcgagcgc ctgtattgat gtcactaccc atgcccccat
    20401 tccgggccag gcaaaaacgg ttccggtgag cactgcgcca tacaacaaat cgcccagcgc
    20461 cagacctaat acggtaatcg atgggatcaa cgcattgggt agcgcataac atagaacgat
    20521 ataccagccg ggcaagccgc tggcccgggc ggtacgaatg tagtcttcac tcaattgttc
    20581 cagcatcgct gagcggatct ggcgggcgac aatccccagg tgaacaaacg ccagcgttaa
    20641 tgccggtaag atgagatgtt gcaacgcatt gaagaagact tcaccgttgc cttcaagcaa
    20701 cgcatcgagc agataaaagc cggtaacgtg cgttggtgga tccagccagt catcaagcct
    20761 tccgccgccg ggaagaattt gcagatgacc ataaaacagc acaatgacgc ccagcccaag
    20821 ccagaacgca ggtgtggaga ttccggtaat ggccattatt cgcaccagat gatccagcca
    20881 gcgatttcgc cagactgcag agagtatgcc cagcgggatg ccaatcagga gtgccagtag
    20941 cagcgcgcaa aatgccagtt ccagcgtcgc cgggaagaat atgcgcaact cttccagcac
    21001 cggacgcccg gtacgaatgg atgtccccag gtcaccatga aacagatcgc tgacgtagcg
    21061 gtaaaactgt acgtacagtg gctggtccag ccccagttgc tggcgaatat tttccacgat
    21121 agcatcgctg gcgcggtcac ccgccagtaa ccgcgccgga tcaccaggga tcagatgcga
    21181 gataataaac gtaatcacgc agacgcccgc caccacgagc accagccccc agcagcgttg
    21241 gcgtaaaata ctccagaagg tcacgttatt tactcatggt attgatattg aagacctgtt
    21301 ccagcatggg attgaacaca aagcctttca cctctttgtt catcgccagt tggtagtttt
    21361 tctggaacag gtacacataa gcagcgtcat caatgacgat tttctgtgcc tgctggtagt
    21421 ctcgggtacg ctgcgtctgg tcggtggtcg cgagcgcatt gcgcagtaac ttatcgacct
    21481 cactgttttc atagaacgag cggttacccg gcagaccttt tttgtcggac tcaaaccagt
    21541 aattcataaa catatacggg tcggcaaaat ccggactcca gttgccaatc gcaatgtcgt
    21601 aatcaccttt acccactctg tcgcgcatgg tggcgttcgc cagcttttcc agcttcacat
    21661 tgatgcccag cttgttgaga ctggattgtg tcgccagagc aataggctcc cagttcggat
    21721 cgttatcgga gtagagaaac gtcaggatgg tgggtttgct cgtcacttta tcccattcag
    21781 ctttggcttt cgtttcgtca tggttgtatt gcattgccgt cgcatcgtag ccccacatgc
    21841 cttccggaat cgggccgcgc atctgttttc cgttaccact cagaatgccg ttaaccatgc
    21901 cctgataatc ggtagaccag gaaatggccc gacgcagatc agcctgatta agtggcgctt
    21961 tgctgttatt cagatacaga taggtgacgc gcagtgacgg atactctgcc acattgattt
    22021 tattttcctg cttcagggcg ttgagttgat ccaccggcag cgcatcggca atgtcaatgt
    22081 cgccacggga gagctgcagg cgacgggagg cactttcacc gataattttt accgatactc
    22141 gcttaaagtt cggtttattg ccggagtaat gcggatttgg caccagaact aattgctgac
    22201 ctttttgcca gcttttcagc ataaatggtc cggaaccggc ggtattttgc gcgaggaagc
    22261 cgcgagcatc atccgctgca tgttccttta agaccgccgg attgataatg gatgcaccgt
    22321 cattcgccag cgtgtagagg aacggtgcaa atggctggct aagggtaaac ttcaccgtat
    22381 gttcgtcggg agcatcaatc tttaaatctt tgggaaatgc ttctgctggc ccctggccga
    22441 tttttagtag ccgctcaaaa gaaagtttta ctgcttcggc agtgacaggt gtgccatcgg
    22501 caaatttagc attatctttc agggtgaacg tccactcttt ttgatcgtca gacgctttcc
    22561 agctacttgc cagatcgcct tcaacgtcgg ttgagccttt atcaccgtcc gttttgtact
    22621 gaaccagtcg ctgataagac gggtaggtca ccgtccagtc gttattatct attgttaccg
    22681 ccgggtcgag ggtttgtgga tcggcggcct taccaatcac cagcatatct ttcggtacgg
    22741 cggcgtgcgc aaccgggaaa gttgtggcaa ggacgagcgc gagcaatgtg ggacgaaacg
    22801 atatcgatct cttcatgaca gtttcctgat taactgacgt gctgtgtgcc aggggatata
    22861 aaacaagaga attgatcagc gagcagaggg taactcgctg cctggggtaa ttcgaagtgc
    22921 caccattcgc tggagatacc gacaaaaccg ccaccagtca taatcgcatt cagcaacagc
    22981 cgattgcgct gagcggcggg cgggacggaa ggatggtaag catgggaacg ttcgtgcatt
    23041 tcatcgaatc ctgcgcccat atcgaggatg ttcccgtgct catcacgaag cgtcaggtcg
    23101 atcgccgtgc cacggctgtg attagaaccg accgtcacat caacaacata ttgcgggtct
    23161 gggcaggctt gccacaacat cgcctgtgct tgttgtgggc gatacgcatc gtaaatcacc
    23221 agttgtaagc ctgatagctg ggcgatactg atacttttcg ccagcgcggt aatcgcatcc
    23281 ttgtgtagca gacaacgcgc ttgctgataa atagctttac ctgtgatgtt atcagcgcag
    23341 gcgtatttca attcgatctc cagatcaggg aaggtcacgg ctaaatcaac cagttcggtg
    23401 gtatccgaca taaacggttc ctgtataaga caaaaattgc tgcgctttcc acttatgcag
    23461 atctcatgcc atgccgggat aagcgccaga atgctggctg aaagttatat tttttaactt
    23521 ttgatcaaca tttgtgcagc gtagtgcagt tttggtgcaa gaggggaagt taaggaagga
    23581 atctcccgga atcgtagctg aaatcacagt atttaagtga cagtgtcacg ttaaatgaaa
    23641 acccgcgagt gcgggcgaga ggaatttgtc agattttcag cggtaacacg ctgctcatcc
    23701 agcctggaat ttcttcggcg ggtagggggc gggaaaagaa atatccctga ataacgcgac
    23761 agtggatctt gcgtagcatc tcaaattgct ctttggtttc gacgccttcc gccacgacgg
    23821 ttaaattgag gctttgccca atgctggtaa tggcttcaag taaggcaagg atgcgttttt
    23881 ccgtcagaca acgatcgaca aaacttttgt caattttgat ttccgttacc ggaagactga
    23941 ctaagcggga taatccggaa aagcccgtgc caaaatcatc taccgataaa cccacgccca
    24001 tatcacgcag gatctgaatg cgcttaaaga tttcggtatc gtgttccatc atcatgcttt
    24061 ccgtgatttc taccgtcagc tggtggccgt caatacccca ggcgtgcatt gcatcagata
    24121 cctgattagg cagctgatta ctgcgaaagt gcagcgccga caagttcacg gataacgccg
    24181 ggatatgaat attctggcta cgccattctg ctaactgacg gcaagcttcc gcgatgaccc
    24241 agcgcccaat attttcgatt tcaccaatct cttctgcgag aggaataaac cgtgaagggg
    24301 gcacatgacc atgctgggga tcgtgccagc gagcaagggc ttcgatgccg tagagttcac
    24361 ccgtttctgc gaagatttgc ggctggtaaa ccagtttcag ttggttattg ctaatcgcct
    24421 ctttcagcgc tgcgcctaaa accaaacgct cttttaccat ttcgttcatc gccgggctga
    24481 agaactgcca gccgttaccg ccattcttgc gaatataatc cattgcattg tgagcagtgg
    24541 agagcaggta atcgcggttt ttacccacgt cgtagctgat gccaatactc aaggtaagcg
    24601 ggaagggttt atcgtcaatc attatcggct tgctgaccac attccgtagc tcatcggcga
    24661 tttgggtaat gttactgacg tcgttttcca ggctcacgag gacaaactgc gtaccttcga
    24721 tacgacagag atactgatcc ggtttgagtt tttcacgaaa gcgattgacc acttccagca
    24781 atgcctgatc ggcccacgca tagccaaggc tatcaatcac atcctgaata tggtcaacac
    24841 cgatgagata caccaccgga gagacggctt tgtcgaccag gtcatcgagg taattgtgca
    24901 ggttattgcg atttggcaga ccggtcatcg gatcaaattg gatgagttgt tcaatatgct
    24961 gacggctttt ttcctgttcc agcgccagcg cggccatatg ctggctgata tctgccacgc
    25021 gttcgataaa ggcgctggtt tctgctcctg aggaggtttt aatttgcagg atccccgcag
    25081 gcgcgccatc acgctgacga atggtcgctg accagctttg cgcattttga acttctgcac
    25141 cgtgcgaaga tgacgcccag tgtatcggca tcccgttgcg cagtgcgaac agcgaaacat
    25201 gcgattcgtt gagaacagat tcgatattac gacaaatgat ttcccccatt tcatgaaatg
    25261 gcggactgct gcacatggcg gcgagaatat tgccttcaag ctggcggatt tgccgttctt
    25321 cggtgatatc cgagaaagtc attaccaggt tctgcagatg cgcgagcacg tcataaaccg
    25381 ggctgataga ggctttaatc cagatttttt caccggtgcg cgtcaacagc agaaattcgt
    25441 cctgatcgcg ggcggttttc catagcaact gttgtaaacg aatgcggtta tcggcaggga
    25501 attcaggaat gttcaggagt gtatcgggct gcataccgct ggcttcgcta atgcagtaac
    25561 caaacatttc ggtaaatgcg cgattgcact gcacaatatg gcgttccgga tcgaggacaa
    25621 tcaccggtcg gtcgagatgg tcaacggcaa taatcaattg tctggtctgt tctttttgcg
    25681 ccatttctac gctggcatcc cgtaccagcg ccaggtaata aactttcccc tcggcgctca
    25741 ctttcgatag cgcaaaacgg gtccagattt tactgccgtc ttttttctcc agctgcagct
    25801 cccgactcat cccctcgacg cgcgctttac cgccttcacg gttgtgacga atgtattcag
    25861 gatgcgcagg acgcaaatcc cgcggaatca gcatatcaat gttattgcca atgacttctt
    25921 cacgtttgta cccccagagc ttctctgcgg cggggttgaa aaacatcact tcatcatttt
    25981 cgttaattaa caccgcaccc atcatatttt gctcaagggc ggggaaaaaa atgccatcgg
    26041 cggcattatc cgcatcggtt agcttcatga ttacctctgc atcctggcgc atctaaagac
    26101 tggctttcca gagttcaaca cggtttctac ctcgtctttt ggcgatatac agagcttcat
    26161 cggctatttg aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg
    26221 aaagtgagag ggcaatatcc tcgccgtttg cggctttcag tttggttttc tccacccgac
    26281 tgcgaatacg ttctgcggta cgtaacgttt cgttttcaga agcttcagtc aaaacaatga
    26341 taaattcatc gcccacgtat cgttgatctc tttgaattta tcaacgtcaa taatcagcac
    26401 tgacagcggt gtaccggtcc ggttggcatg ggcaatttcg cgtttgaaga tagtcggcag
    26461 gaaacggcgg ttaagtaatt tcgtcagtac atccataccg acttcgtggc gcgatacttc
    26521 ttcaaacaat tcacgcagca aggtaataat ttgcgataca gt
//
LOCUS       DAZUGK010000044        26405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZUGK010000044 DAZUGK010000000
VERSION     DAZUGK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.044"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            225..419
                     /gene="bfd"
                     /locus_tag="ACHY5W_001633"
     CDS             225..419
                     /gene="bfd"
                     /locus_tag="ACHY5W_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFS4446545.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            492..968
                     /gene="bfr"
                     /locus_tag="ACHY5W_001634"
     CDS             492..968
                     /gene="bfr"
                     /locus_tag="ACHY5W_001634"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFS4446546.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(965..1432)
                     /locus_tag="ACHY5W_001635"
     CDS             complement(965..1432)
                     /locus_tag="ACHY5W_001635"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4446547.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1811..2122
                     /gene="rpsJ"
                     /locus_tag="ACHY5W_001636"
     CDS             1811..2122
                     /gene="rpsJ"
                     /locus_tag="ACHY5W_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4446548.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2155..2784
                     /gene="rplC"
                     /locus_tag="ACHY5W_001637"
     CDS             2155..2784
                     /gene="rplC"
                     /locus_tag="ACHY5W_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4446549.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2795..3400
                     /gene="rplD"
                     /locus_tag="ACHY5W_001638"
     CDS             2795..3400
                     /gene="rplD"
                     /locus_tag="ACHY5W_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4446550.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3397..3699
                     /gene="rplW"
                     /locus_tag="ACHY5W_001639"
     CDS             3397..3699
                     /gene="rplW"
                     /locus_tag="ACHY5W_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4446551.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3717..4538
                     /gene="rplB"
                     /locus_tag="ACHY5W_001640"
     CDS             3717..4538
                     /gene="rplB"
                     /locus_tag="ACHY5W_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4446552.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4555..4833
                     /gene="rpsS"
                     /locus_tag="ACHY5W_001641"
     CDS             4555..4833
                     /gene="rpsS"
                     /locus_tag="ACHY5W_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4446553.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4848..5180
                     /gene="rplV"
                     /locus_tag="ACHY5W_001642"
     CDS             4848..5180
                     /gene="rplV"
                     /locus_tag="ACHY5W_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4446554.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5198..5899
                     /gene="rpsC"
                     /locus_tag="ACHY5W_001643"
     CDS             5198..5899
                     /gene="rpsC"
                     /locus_tag="ACHY5W_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4446555.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5912..6322
                     /gene="rplP"
                     /locus_tag="ACHY5W_001644"
     CDS             5912..6322
                     /gene="rplP"
                     /locus_tag="ACHY5W_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4446556.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6322..6513
                     /gene="rpmC"
                     /locus_tag="ACHY5W_001645"
     CDS             6322..6513
                     /gene="rpmC"
                     /locus_tag="ACHY5W_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4446557.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6513..6767
                     /gene="rpsQ"
                     /locus_tag="ACHY5W_001646"
     CDS             6513..6767
                     /gene="rpsQ"
                     /locus_tag="ACHY5W_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4446558.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6932..7303
                     /gene="rplN"
                     /locus_tag="ACHY5W_001647"
     CDS             6932..7303
                     /gene="rplN"
                     /locus_tag="ACHY5W_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4446559.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7314..7628
                     /gene="rplX"
                     /locus_tag="ACHY5W_001648"
     CDS             7314..7628
                     /gene="rplX"
                     /locus_tag="ACHY5W_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4446560.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7643..8182
                     /gene="rplE"
                     /locus_tag="ACHY5W_001649"
     CDS             7643..8182
                     /gene="rplE"
                     /locus_tag="ACHY5W_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4446561.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8197..8502
                     /gene="rpsN"
                     /locus_tag="ACHY5W_001650"
     CDS             8197..8502
                     /gene="rpsN"
                     /locus_tag="ACHY5W_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4446562.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8536..8928
                     /gene="rpsH"
                     /locus_tag="ACHY5W_001651"
     CDS             8536..8928
                     /gene="rpsH"
                     /locus_tag="ACHY5W_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4446563.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8941..9474
                     /gene="rplF"
                     /locus_tag="ACHY5W_001652"
     CDS             8941..9474
                     /gene="rplF"
                     /locus_tag="ACHY5W_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4446564.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9484..9837
                     /gene="rplR"
                     /locus_tag="ACHY5W_001653"
     CDS             9484..9837
                     /gene="rplR"
                     /locus_tag="ACHY5W_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4446565.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9852..10355
                     /gene="rpsE"
                     /locus_tag="ACHY5W_001654"
     CDS             9852..10355
                     /gene="rpsE"
                     /locus_tag="ACHY5W_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4446566.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10359..10538
                     /gene="rpmD"
                     /locus_tag="ACHY5W_001655"
     CDS             10359..10538
                     /gene="rpmD"
                     /locus_tag="ACHY5W_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4446567.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10542..10976
                     /gene="rplO"
                     /locus_tag="ACHY5W_001656"
     CDS             10542..10976
                     /gene="rplO"
                     /locus_tag="ACHY5W_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4446568.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10984..12315
                     /gene="secY"
                     /locus_tag="ACHY5W_001657"
     CDS             10984..12315
                     /gene="secY"
                     /locus_tag="ACHY5W_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4446569.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12347..12463
                     /gene="rpmJ"
                     /locus_tag="ACHY5W_001658"
     CDS             12347..12463
                     /gene="rpmJ"
                     /locus_tag="ACHY5W_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4446570.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12610..12966
                     /gene="rpsM"
                     /locus_tag="ACHY5W_001659"
     CDS             12610..12966
                     /gene="rpsM"
                     /locus_tag="ACHY5W_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4446571.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12983..13372
                     /gene="rpsK"
                     /locus_tag="ACHY5W_001660"
     CDS             12983..13372
                     /gene="rpsK"
                     /locus_tag="ACHY5W_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4446572.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13406..14026
                     /gene="rpsD"
                     /locus_tag="ACHY5W_001661"
     CDS             13406..14026
                     /gene="rpsD"
                     /locus_tag="ACHY5W_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4446573.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14052..15041
                     /gene="rpoA"
                     /locus_tag="ACHY5W_001662"
     CDS             14052..15041
                     /gene="rpoA"
                     /locus_tag="ACHY5W_001662"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4446574.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15082..15465
                     /gene="rplQ"
                     /locus_tag="ACHY5W_001663"
     CDS             15082..15465
                     /gene="rplQ"
                     /locus_tag="ACHY5W_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4446575.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15572..15940
                     /locus_tag="ACHY5W_001664"
     CDS             15572..15940
                     /locus_tag="ACHY5W_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFS4446576.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15951..16376
                     /gene="zntR"
                     /locus_tag="ACHY5W_001665"
     CDS             15951..16376
                     /gene="zntR"
                     /locus_tag="ACHY5W_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFS4446577.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16432..16650
                     /gene="arfA"
                     /locus_tag="ACHY5W_001666"
     CDS             16432..16650
                     /gene="arfA"
                     /locus_tag="ACHY5W_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFS4446578.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16647..17057)
                     /gene="mscL"
                     /locus_tag="ACHY5W_001667"
     CDS             complement(16647..17057)
                     /gene="mscL"
                     /locus_tag="ACHY5W_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4446579.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNHS"
     gene            complement(17187..18563)
                     /gene="trkA"
                     /locus_tag="ACHY5W_001668"
     CDS             complement(17187..18563)
                     /gene="trkA"
                     /locus_tag="ACHY5W_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFS4446580.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18585..19874)
                     /gene="rsmB"
                     /locus_tag="ACHY5W_001669"
     CDS             complement(18585..19874)
                     /gene="rsmB"
                     /locus_tag="ACHY5W_001669"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4446581.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19920..20867)
                     /gene="fmt"
                     /locus_tag="ACHY5W_001670"
     CDS             complement(19920..20867)
                     /gene="fmt"
                     /locus_tag="ACHY5W_001670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4446582.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PHLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20882..21391)
                     /gene="def"
                     /locus_tag="ACHY5W_001671"
     CDS             complement(20882..21391)
                     /gene="def"
                     /locus_tag="ACHY5W_001671"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4446583.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21521..22645
                     /gene="dprA"
                     /locus_tag="ACHY5W_001672"
     CDS             21521..22645
                     /gene="dprA"
                     /locus_tag="ACHY5W_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFS4446584.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22617..23090
                     /gene="smg"
                     /locus_tag="ACHY5W_001673"
     CDS             22617..23090
                     /gene="smg"
                     /locus_tag="ACHY5W_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFS4446585.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23119..23661
                     /locus_tag="ACHY5W_001674"
     CDS             23119..23661
                     /locus_tag="ACHY5W_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFS4446586.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23666..24238
                     /gene="tsaC"
                     /locus_tag="ACHY5W_001675"
     CDS             23666..24238
                     /gene="tsaC"
                     /locus_tag="ACHY5W_001675"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFS4446587.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24243..25061
                     /gene="aroE"
                     /locus_tag="ACHY5W_001676"
     CDS             24243..25061
                     /gene="aroE"
                     /locus_tag="ACHY5W_001676"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4446588.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25058..25315
                     /locus_tag="ACHY5W_001677"
     CDS             25058..25315
                     /locus_tag="ACHY5W_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFS4446589.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25291..25845)
                     /locus_tag="ACHY5W_001678"
     CDS             complement(25291..25845)
                     /locus_tag="ACHY5W_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4446590.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVEMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26318..>26405
                     /locus_tag="ACHY5W_001679"
     rRNA            26318..>26405
                     /locus_tag="ACHY5W_001679"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6804 a         6240 c         6831 g         6530 t
ORIGIN      
        1 cgtaccgttg gcgcgggcgt tgtagctaaa gttctgggct aattacacgt taattagttt
       61 tgaattgaaa agggcgcttc ggcgcccttt ttgctgcccg taatttgagc tcgaaatatt
      121 tagtcgtaat tttgctcgct gaactatggc gcaaataaaa ctgtgctatt gtaatcataa
      181 ctattctcat ttacactttg cgcagaattt gaacgggagc aatcatgtac gtttgtttgt
      241 gtaatggtgt aagtgacaaa aaaattcgcc aggcagtccg tcagtttcat ccgcaatctt
      301 tccaacaatt gcgtaagttt attcctgtgg gaaatcaatg tggtaagtgc attcgcgccg
      361 cgcgcgaagt gatgcaggac gagttgatgc agatgccaga atttaaagag attgcctgaa
      421 gcccacgctt attttttgac atcccgctag ccccatctac gctctaatag tggaagcgga
      481 gggactataa aatgaaaggt gatgttaaaa tcataaatta tctcaataaa ctattgggaa
      541 atgagcttgt cgcaatcaat cagtattttc tccatgcccg gatgtttaaa aactggggac
      601 tgatgcgtct taatgacgtc gaataccatg aatccatcga tgagatgaaa cacgctgata
      661 aatatatcga gcgtattttg tttttagaag gaatccccaa cttacaggat ttaggtaagc
      721 tagggatcgg tgaagatgtc gaagagatgc tgcaatccga cctgcgatta gagctggaag
      781 gtgcgaagga tctgcgtgag gctatcgcgt atgccgacag cgttcatgac tatgttagcc
      841 gcgatatgat gatcgagatc cttgccgatg aagaaggtca tattgactgg ctcgaaacag
      901 agttagatct cattggcaaa attggcctgc aaaattacct gcaatcacaa attaaagtta
      961 aagattaacg gcctatcagc aaactatccc agcctataac gaaacccgca gccgccagaa
     1021 atggcccaaa aggcagcggg ttttttaatg attcttttcc tctcatgagc agaccaataa
     1081 ctacggctcc gcaagcgaat gaggcagcaa ggaaaaccag tcgtggtaag aaagcccatg
     1141 aatgccaggc gccaagggcg gcgagaaact tcacatcacc atagcctaat ccttctttat
     1201 ggcgcagtat gcgatacccc cagtaaatga cagcgaatgt gccgtaacca ataattgccc
     1261 cccataacgc atcggctaaa ccatcaggat gacaaacttg atagaacaaa agacctgacc
     1321 agagtaacgg gcacgtaaat ctgtcaggta acaagccatg ctttgcatcc cagaaaaaaa
     1381 gtagggctga taaacaagcg tacaaaatta gaaagggaag ggtagtgtcc ataaaacatc
     1441 ctctgtaatt ggagtatcac catcgaataa ctccggatgc tcagcaacag agagagcgct
     1501 gatttgttaa cctgattccg gtgaaaaacg ctatcttcac aaccttacaa atttcgtgga
     1561 atgcagctcg caatctgcgc gttgcgatta atttctaccc gatgcttgcg tcaggctcag
     1621 atttgcgtat aatgcgcggg cttgtcgtag ttgacagcag gttcaatctg aacctcagtt
     1681 agccgaattt ggctacctaa caatgctccc aatcggggag ctacgtaaga acggttacac
     1741 tctcccatca atcgtaatgg gtctgaggag taatcatttt cgtttataaa ataattggag
     1801 ctctggtctc atgcagaacc aaagaatccg tatccgcctg aaagcgtttg atcatcgtct
     1861 gatcgatcaa gcaaccgcgg aaatcgtcga gactgccaag cgcactggtg cgcaggtccg
     1921 tggtccgatc ccgctgccga cacgcaaaga gcgcttcact gttctgatct ccccgcacgt
     1981 caacaaagac gcgcgcgatc agtacgaaat ccgtactcac ttgcgtctgg ttgacatcgt
     2041 tgagccaacc gagaaaaccg ttgatgctct gatgcgtctg gatctggctg ccggtgtaga
     2101 cgtgcagatc agcctgggtt aatcaggtca ttgagcgatt gagaggttga aacaatgatt
     2161 ggtttagtcg gtaaaaaagt gggtatgacc cgtatcttca cagaagacgg cgtttctatc
     2221 ccagtaaccg taatcgaagt tgaagcaaac cgcgttactc aggttaaaga cctggctaac
     2281 gatggctacc gtgctattca ggtgaccacc ggtgctaaaa aagctaaccg tgtgaccaag
     2341 cctgaagctg gccacttcgc taaagctggc gtagaagctg gccgtggtct gtgggaattc
     2401 cgcctggctg aaggcgaaga gttcactgta ggtcagagca ttagcgttga actgtttgct
     2461 gacgttaaaa aagttgacgt aactggcacc tctaaaggta aaggtttcgc aggtaccgtt
     2521 aagcgctgga acttccgtac ccaggacgct actcacggta actccttgtc tcaccgcgtt
     2581 ccgggttcta tcggtcagaa ccagactccg ggcaaagtgt tcaaaggcaa gaaaatggca
     2641 ggtcagatgg gtaacgaacg tgtaaccgtt cagagccttg acgtagtacg cgttgacgct
     2701 gagcgcaacc tgctgctggt taaaggtgct gtcccgggtg caaccggtag cgacctgatc
     2761 gttaaaccag ctgtgaaggc gtaaggagat agcaatggaa ttagtattga aagacgcgca
     2821 gagcgcgctg actgtttccg aaactacctt cggtcgtgat ttcaacgaag cgctggttca
     2881 ccaggttgtt gttgcttatg cagctggtgc tcgtcagggt actcgtgctc agaagactcg
     2941 tgctgaagta actggttccg gtaaaaaacc gtggcgccag aaaggcaccg gccgtgcgcg
     3001 ttctggttct atcaagagcc cgatctggcg ttctggtggc gtgacctttg ctgctcgtcc
     3061 gcaggaccac agtcaaaaag ttaacaagaa gatgtaccgc ggcgcgctga aaagcatcct
     3121 gtccgaactg gtacgtcagg atcgtctgat cgttgtcgag aagttctctg tagaagcgcc
     3181 gaaaactaag ctgctggcac agaaactgaa agacatggct ctggaagatg tgctgatcat
     3241 caccggtgag ctggacgaaa acctgttcct ggctgcgcgc aacctgcaca aggttgacgt
     3301 acgcgatgca actggtatcg acccggttag cctgatcgcc ttcgacaaag tcgtaatgac
     3361 tgctgatgct gttaagcaag ttgaggagat gctggcatga ttcgtgaaga acgtctgctg
     3421 aaggtgctgc gtgcaccgca cgtttctgaa aaagcgtcta ctgcgatgga aaaatccaac
     3481 accatcgtac tcaaagttgc taaagacgcg accaaagcag aaatcaaagc tgctgtgcag
     3541 aaactgtttg aagtcgaagt cgaagtcgtt aacaccctgg tagttaaagg gaaagttaaa
     3601 cgtcacggac agcgtatcgg tcgtcgtagc gactggaaaa aagcttacgt caccctgaaa
     3661 gaaggccaga atctggactt cgttggcggc gctgagtaag tcggaggagt aatacaatgg
     3721 cagttgttaa atgtaaaccg acatctccgg gtcgtcgcca cgttgttaaa gtggttaacc
     3781 ctgagctgca caagggcaaa ccttttgctc cgttgctgga aaaaaacagc aaatccggtg
     3841 gtcgtaacaa caatggccgt atcaccactc gtcatatcgg tggtggccac aagcaggctt
     3901 accgtattgt tgacttcaaa cgcaacaaag acggtatccc ggcagttgtt gaacgtcttg
     3961 agtacgatcc gaaccgttcc gcgaacatcg cgctggttct gtacaaagac ggtgaacgcc
     4021 gttacatcct ggcccctaaa ggcctgaaag ctggcgacca gattcagtct ggcgttgatg
     4081 ctgcaatcaa accaggtaac accctgccga tgcgcaacat cccggttggt tctactgttc
     4141 ataacgtaga aatgaaacca ggtaaaggcg gtcagctggc acgttccgct ggtacttacg
     4201 ttcagatcgt tgctcgtgat ggtgcttatg tcaccctgcg tctgcgttct ggtgaaatgc
     4261 gtaaagtaga agcagactgc cgtgcaactc tgggcgaagt tggcaatgct gagcatatgc
     4321 tgcgcgttct gggtaaagca ggtgctgcac gctggcgtgg tgttcgtccg accgttcgtg
     4381 gtaccgcgat gaacccggta gaccacccac atggtggtgg tgaaggtcgt aactttggta
     4441 agcacccggt aactccgtgg ggcgttcaga ccaaaggtaa gaagacccgc agcaacaagc
     4501 gtactgataa attcatcgta cgtcgccgta gcaaataatt ttagaggata agccatgcca
     4561 cgttctctca agaaaggtcc ttttattgac ctgcacttgc tgaagaaggt agagaaagcg
     4621 gtggaaagcg gagacaagaa gcccctgcgc acttggtccc gtcgttcaac gatctttcct
     4681 aacatgatcg gtttgaccat cgctgtccat aatggtcgtc agcacgttcc ggtatttgta
     4741 accgacgaaa tggtcggtca caaactgggt gaattcgcac cgactcgtac ttatcgcggc
     4801 cacgctgctg ataaaaaagc gaagaagaaa taaggtagga ggaagagatg gaaactatcg
     4861 ctaaacatcg ccatgctcgt tcttctgctc agaaggttcg ccttgttgct gacctgattc
     4921 gcggtaagaa agtgtcgcag gctctggata ttttgaccta caccaacaag aaagcggctg
     4981 tactggtcaa gaaagttctg gaatctgcca ttgctaacgc tgaacacaac gatggcgctg
     5041 acattgacga tctgaaagtt acgaaaattt tcgtagacga aggcccgagc atgaagcgca
     5101 ttatgccgcg tgcaaaaggt cgtgcagatc gcatcctgaa gcgcaccagc cacatcactg
     5161 tggttgtgtc cgatcgctga gactctggag actagcaatg ggtcagaaag tacatcctaa
     5221 tggtattcgc ctgggtattg taaaaccatg gaactctacc tggtttgcga acaccaaaga
     5281 attcgctgac aacctggaca gcgattttaa agtacgtcag tacctgacta aggaactggc
     5341 taaagcgtcc gtatctcgta tcgttatcga gcgtccggct aagagcatcc gtgtaaccat
     5401 tcacactgct cgcccgggta tcgttatcgg taaaaaaggt gaagacgtag aaaaactgcg
     5461 taaggtcgta gcggacatcg ctggcgttcc tgcacagatc aacatcgccg aagttcgtaa
     5521 gcctgaactg gacgcaaaac tggttgctga cagcatcact tctcagctgg aacgtcgcgt
     5581 tatgttccgt cgtgctatga agcgtgctgt acagaacgca atgcgtctgg gcgctaaagg
     5641 tattaaagtt gaagttagcg gccgtctggg cggcgcggaa atcgcacgta ccgaatggta
     5701 ccgcgaaggt cgcgtaccgc tgcacactct gcgtgctgac atcgactaca acacctctga
     5761 agcgcacacc acttacggtg taatcggcgt taaagtgtgg atcttcaaag gcgagatcct
     5821 gggtggtatg gctgctgttg aacaaccgga aaaaccggct gctcagccta aaaagcagca
     5881 gcgtaaaggc cgtaaataag gagcgtcgct gatgttacaa ccaaagcgta caaaattccg
     5941 taaaatgcac aaaggccgta accgcggtct ggcgcagggt acggatgtta gcttcggcag
     6001 cttcggtctg aaagctgttg gccgtggtcg tctgactgcc cgtcagatcg aagcagcacg
     6061 tcgtgctatg acccgtgcag ttaagcgtca aggtaagatc tggatccgtg tgttcccgga
     6121 caaaccgatc actgaaaagc cgctggcagt gcgtatgggt aaaggtaaag gtaacgtgga
     6181 gtattgggtt gccttgattc agccgggtaa agtcctgtat gaaatggacg gtgttccgga
     6241 agagctggcc cgtgaagcat tcaagctggc agcagcgaaa ctgccgatta aaaccacctt
     6301 tgtaactaag acggtgatgt aatgaaagca aaagagctgc gtgagaagag cgttgaagag
     6361 ctgaacaccg agctgctgaa cctgctgcgt gagcagttca acctgcgtat gcaggctgca
     6421 agtggccagc tgcaacagtc tcacctgttg aagcaagtgc gtcgcgatgt cgcacgcgtt
     6481 aagactttac tgaacgagaa ggcgggtgcg taatgaccga taaaatccgt actctgcaag
     6541 gtcgcgttgt tagcgacaaa atggagaaat ccattgttgt tgctatcgaa cgttttgtga
     6601 aacacccgat ctacggtaaa ttcatcaagc gtacgaccaa actgcacgta catgacgaga
     6661 acaacgaatg cggtatcggt gacgtggttg aaatccgcga atgccgtccg ctgtccaaga
     6721 ctaaatcctg gacgctggtt cgcgttgtag agaaagcggt tctgtaatac agtacactct
     6781 ctcgatacga ataaacggct cagaaatgag ccgtttattt tttctaccca tatccttgaa
     6841 gcggtgttat aatgccgcgc cctcgatatg gggattttta acgacctgat tttcgggtct
     6901 cagtagtagt tgacattagc ggagcactaa aatgatccaa gaacagacta tgctgaacgt
     6961 cgccgacaac tccggtgcac gtcgcgtaat gtgtatcaag gttctgggtg gctcgcaccg
     7021 tcgctacgca ggcgtaggcg acatcatcaa gatcaccatc aaagaagcaa ttccgcgtgg
     7081 taaggtcaaa aaaggtgatg tgctgaaggc ggtagtggtg cgcaccaaga agggtgttcg
     7141 tcgcccggac ggttctgtca ttcgcttcga tggtaatgct tgtgttcttc tgaacaacaa
     7201 cagcgagcag cctatcggta cgcgtatttt tgggccggta actcgtgagc ttcgtagtga
     7261 gaagttcatg aaaattatct ctctggcacc agaagtactc taaggagcga atcatggcag
     7321 cgaaaatccg tcgtgatgac gaagttatcg tgttaaccgg taaagataaa ggtaaacgcg
     7381 gtaaagttaa gaatgtcctg tcttccggca aggtcattgt tgaaggtatc aacctggtta
     7441 agaaacatca gaagccggtt ccggccctga accaaccggg tggcatcgtt gaaaaagaag
     7501 ccgctattca ggtttccaac gtagcaatct tcaatgcggc aaccggcaag gctgaccgtg
     7561 taggctttag attcgaagac ggcaaaaaag tccgtttctt caagtctaac agcgaaacta
     7621 tcaagtaatt tggagtagta cgatggcgaa actgcatgat tactacaaag acgaagtagt
     7681 taaaaaactc atgactgagt ttaactacaa ttctgtcatg caagtccctc gggtcgagaa
     7741 gatcaccctg aacatgggtg ttggtgaagc gatcgctgac aaaaaactgc tggataacgc
     7801 agcagcagac ctggcagcaa tctccggtca aaaaccgctg atcaccaaag cacgcaaatc
     7861 tgttgcaggc ttcaaaatcc gtcagggcta tccgatcggc tgtaaagtaa ctctgcgtgg
     7921 cgaacgcatg tgggagttct ttgagcgcct gatcactatt gctgtacctc gtatccgtga
     7981 cttccgtggc ctgtccgcta agtctttcga cggtcgtggt aactacagca tgggtgtccg
     8041 tgagcagatc atcttcccag aaatcgacta cgataaagtc gaccgcgttc gtggtttgga
     8101 tattaccatt accactactg cgaaatctga cgaagaaggc cgcgctctgc tggctgcctt
     8161 tgacttcccg ttccgcaagt aaggtagggt tactaaatgg ctaagcaatc aatgaaagca
     8221 cgcgaagtaa aacgcgtagc tttagctgat aaatacttcg cgaaacgcgc tgaactgaaa
     8281 gcgatcatct ctgatgtgaa cgcttccgac gaagatcgtt ggaacgctgt tctcaagctg
     8341 cagactctgc cgcgtgattc cagcccgtct cgtcagcgta accgctgccg tcaaacaggt
     8401 cgtccgcatg gtttcctgcg gaagttcggg ttgagccgta ttaaggtccg tgaagccgct
     8461 atgcgcggtg aaatcccggg tctgaaaaag gctagctggt aattgtcacc aattgaatca
     8521 cgggagtaaa gacagatgag catgcaagat ccgatcgcgg atatgctgac ccgtatccgt
     8581 aacggtcagg ccgcgaacaa agctgcggtc accatgcctt cctccaagct gaaagtggca
     8641 atcgccaacg tgctgaagga agaaggtttt attgaagatt ttaaagttga aggcgacacc
     8701 aagcctgaac tggaacttac tctgaagtat ttccagggca aagctgttgt agaaagcatt
     8761 cagcgtgtca gccgcccagg tctgcgcatc tataaacgta aagatgagct gccgaaagtt
     8821 atggcgggtc tgggtatcgc agttgtttct acctctaaag gtgttatgac tgatcgtgca
     8881 gcgcgccagg ctggtcttgg tggcgaaatt atctgctacg tagcctaatc ggaggaaaaa
     8941 atgtctcgtg ttgctaaagc accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac
     9001 ggtcaggtta ttacgatcaa aggtaaaaac ggcgagctga ctcgtactct caacgatgct
     9061 gttgaagtta aacatgcaga taataccctg accttcggtc cgcgtgatgg ttacgcagac
     9121 ggttgggcac aggctggtac cgcgcgtgcc ctgctgaact caatggttat cggtgttacc
     9181 gaaggcttca ctaagaagct gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc
     9241 aatgtgatta acctgtctct gggtttctct catcctgttg accatcagct gcctgcgggt
     9301 atcactgctg aatgtccgac tcagactgaa atcgtgctga aaggcgctga taagcaggtg
     9361 atcggccagg ttgcagcgga tctgcgcgcc taccgtcgtc ctgagcctta taaaggcaag
     9421 ggtgttcgtt acgccgacga agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac
     9481 actatggata agaaatctgc tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag
     9541 gagctgggcg caactcgcct ggtggtacat cgtaccccgc gtcacattta cgcacaggta
     9601 attgcaccga acggttctga agttctggta gctgcttcta ctgtagaaaa agctatcgct
     9661 gaacaactga agtacaccgg taacaaagac gcggctgcag ctgtgggtaa agctgtcgct
     9721 gaacgcgctc tggaaaaagg catcaaagat gtatcctttg accgttccgg gttccaatat
     9781 catggtcgtg tccaggcact ggcagatgct gcccgtgaag ctggccttca gttctaaggt
     9841 agaggtgtaa gatggctcac atcgaaaaac aagctggcga actgcaggaa aagctgatcg
     9901 cggtaaaccg cgtatctaaa accgttaaag gtggtcgtat tttctccttc acagctctga
     9961 ctgtagttgg cgatggtaac ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc
    10021 cagcagcgat ccagaaagcg atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga
    10081 ataacggcac tctgcaacac cctgttaaag gtgttcacac gggttctcgc gtattcatgc
    10141 agccggcttc cgaaggtacc ggtatcatcg ccggtggtgc aatgcgcgcc gttctggaag
    10201 tcgctggggt tcataacgtt ctggctaaag catatggttc caccaacccg atcaacgtgg
    10261 ttcgtgcaac tattgatggc ctggaaaata tgaattctcc agaaatggtc gctgccaagc
    10321 gtggtaaatc cgttgaagaa attctgggga aataaaccat ggcaaagact attaaaatta
    10381 ctcaaacccg cagtgcaatc ggtcgtctgc cgaaacacaa ggcaacgctg cttggcctgg
    10441 gtctgcgtcg tattggtcac accgtagagc gcgaggatac tcctgctatt cgcggtatga
    10501 tcaacgcggt ttccttcatg gttaaagttg aggagtaaga gatgcgttta aatactctgt
    10561 ctccggccga aggctccaaa aaggcgggta aacgcctggg tcgtggtatc ggttctggcc
    10621 tcggtaaaac cggtggtcgt ggtcacaaag gtcagaagtc tcgttctggc ggtggcgtac
    10681 gtcgcggttt cgagggtggt cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca
    10741 cttctcgtaa agcagcgatt acagccgaag ttcgtctgtc tgacctggct aaagtagaag
    10801 gcggtgttgt agacctgaac acgctgaaag cggctaacat tatcggtatc cagatcgagt
    10861 tcgcgaaagt gatcctggct ggcgaagtaa cgactccggt aactgttcgt ggcctgcgtg
    10921 ttactaaagg cgctcgtgct gctatcgaag ctgctggcgg taaaatcgag gaataagtag
    10981 cagatggcta aacaaccggg attagatttt caaagtgcca aaggtggctt aggcgagctg
    11041 aaacgcagac tgctgtttgt tatcggtgcg ctgattgtgt ttcgtattgg ctcttttatt
    11101 ccgatccctg gtattgatgc cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc
    11161 atcattgaga tgtttaacat gttctctggt ggtgctctca gccgtgcttc tatctttgct
    11221 ctggggatca tgccgtatat ttcggcgtcg atcattatcc agctgctgac ggtggttcac
    11281 ccaacgttgg cagaaattaa gaaagaaggg gagtctggtc gtcgtaagat cagccagtac
    11341 acccgctacg gtactctggt gctggcaata ttccagtcga tcggtattgc taccggtctg
    11401 ccgaatatgc ctggtatgca aggcctggtg attaacccgg gctttgcatt ctacttcacc
    11461 gctgttgtaa gtctggtcac aggaacgatg ttcctgatgt ggttgggcga acagatcact
    11521 gaacgaggta tcggcaacgg tatttcaatc attatcttcg ccggtattgt cgcgggactc
    11581 ccgccagcca ttgcccatac tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg
    11641 ttgctgttgg ttgcagtatt agtatttgca gtgacgttct ttgttgtatt tgttgagcgt
    11701 ggtcaacgcc gcattgtggt aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct
    11761 gcacagagca cacatttacc gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc
    11821 gcttccagta ttattctgtt cccggcgacc atcgcgtcat ggttcggggg cggtactggt
    11881 tggaactggc tgacaacaat ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta
    11941 ctctatgcgt ctgcaatcat cttcttctgt ttcttctaca cggcgttggt tttcaacccg
    12001 cgtgaaacag cagataacct gaagaagtcc ggtgcatttg taccaggaat tcgtccggga
    12061 gagcaaacgg cgaagtatat cgataaagta atgacccgcc tgaccctggt tggtgcgctg
    12121 tatattacct ttatctgcct gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc
    12181 tacttcggtg ggacctcact gcttatcgtt gttgtcgtga ttatggactt tatggctcaa
    12241 gtgcaaactc tgatgatgtc cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa
    12301 ggctacggcc gataattggt cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc
    12361 ttccgtcaag aaattatgcc gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt
    12421 gatttgcagt gccgagccga agcataaaca gcgccaaggc tgattttttc gcatattttt
    12481 cttgcaaagt tgggttgagc tggctagatt agccagccaa tcttttgtat gtctgtgcgt
    12541 ttccatttga gtatcctgaa aacgggcttt tcagcatgga acgtacatat taaatagtag
    12601 gagtgcatag tggcccgtat agcaggcatt aacattcctg atcataagca tgccgtaatc
    12661 gcattaactt cgatttatgg cgtcggcaag acccgttcta aagccatcct ggctgcagcg
    12721 ggtatcgctg aagatgttaa gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt
    12781 gacgaagttg ccaaatttgt cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc
    12841 aagcgcctga tggatcttgg ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt
    12901 cgcggtcagc gtaccaagac caacgcacgt acccgtaagg gtccgcgcaa accgatcaag
    12961 aaataatcgg ggtgattgaa taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag
    13021 aaaacaagtc tctgacggcg tggctcatat ccatgcttct ttcaacaaca ccatcgtgac
    13081 tatcactgat cgtcagggta acgcgttggg ttgggcaaca gccggtggtt ccggtttccg
    13141 tggttctcgc aaatccactc cgtttgcagc tcaggttgca gcagagcgtt gcgctgacgc
    13201 cgtgaaagaa tacggcatca agaatctgga agttatggtt aaaggtccgg gtccaggccg
    13261 cgaatctact attcgtgctc tgaacgccgc aggtttccgc atcactaaca ttactgatgt
    13321 gactccgatc cctcataacg gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt
    13381 tttccaggtt tgttggagaa agaaaatggc aagatatttg ggtcctaagc tcaagctgag
    13441 ccgtcgtgag ggcaccgact tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg
    13501 taaaattgaa caagctcctg gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg
    13561 tgtgcagttg cgtgaaaagc aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt
    13621 ccgtaactac tacaaagaag cagcacgtct gaaaggcaac accggtgaaa acctgttggc
    13681 tctgctggaa ggtcgtctgg acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc
    13741 agaagcacgt cagctggtta gccataaagc aattatggta aacggtcgtg ttgttaacat
    13801 cgcttcttat caggttagtc cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca
    13861 gtctcgcgtg aaagccgctc tggagctggc tgagcagcgt gaaaagccaa cctggctgga
    13921 agttgatgct ggcaagatgg aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc
    13981 tgcggacatt aacgaacacc tgatcgtcga gctttactcc aagtaaagct tagtaccaaa
    14041 gagaggacac aatgcagggt tctgtgacag agtttctaaa accgcgcctg gttgatatcg
    14101 agcaagtgag ttcgacgcac gccaaggtga cccttgagcc tttagagcgt ggctttggcc
    14161 atactctggg taacgcactg cgccgtattc tgctctcatc gatgccgggt tgcgcggtga
    14221 ccgaggttga gattgatggt gtactacatg agtacagcac caaagaaggc gttcaggaag
    14281 atatcctgga aatcctgctc aacctgaaag ggctggcggt gagagttcag ggcaaagatg
    14341 aagttattct taccttgaat aaatctggca ttggccctgt gactgcagcc gatatcaccc
    14401 acgacggtga tgtcgaaatc gtcaagccgc agcacgtgat ctgccacctg accgatgaga
    14461 acgcgtctat tagcatgcgt atcaaagttc agcgcggtcg tggttatgtg ccggcttcta
    14521 cccgaattca ttcggaagaa gatgagcgcc caatcggccg tctgctggtc gacgcatgct
    14581 acagccctgt ggagcgtatt gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg
    14641 acctggacaa gctggtcatc gaaatggaaa ccaacggcac aatcgatcct gaagaggcga
    14701 ttcgtcgtgc ggcaaccatt ctggctgaac aactggaagc tttcgttgac ttacgtgatg
    14761 tacgtcagcc tgaagtgaaa gaagagaaac cagagttcga tccgatcctg ctgcgccctg
    14821 ttgacgatct ggaattgact gtccgctctg ctaactgcct taaagcagaa gctatccact
    14881 atatcggtga tctggtacag cgtaccgagg ttgagctcct taaaacgcct aaccttggta
    14941 aaaaatctct tactgagatt aaagacgtgc tggcttcccg tggactgtct ctgggcatgc
    15001 gcctggaaaa ctggccaccg gcaagcatcg ctgacgagta accggatcac aggttaaggt
    15061 tttactgaga aggataaggt catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac
    15121 agcagccatc gccaggctat gttccgcaat atggcaggtt cactggttcg tcatgaaatc
    15181 atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg tagttgagcc gctgattact
    15241 cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac
    15301 gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt
    15361 tacactcgta ttctgaagtg tggcttccgt gcaggcgaca acgcgccgat ggcttacatc
    15421 gagctggttg atcgttcaga gaaagcagaa gctgctgcag agtaatctga agcaacgtaa
    15481 aaaaacccgc cccggcgggt ttttttatac ccgtagcatc cccacttatc tacaatagct
    15541 gtactctttt tgttcatccc ctggagtatt tatgtggtta cttgaccagt gggcagagcg
    15601 tcatatagca gaagcgcaag cgaaaggtga gtttgataac ctggcaggta gcggcgaacc
    15661 attgatactg gatgatgatt ctcatgtgcc accggaatta cgtgcggggt atcgcttgct
    15721 gaagaatgcc ggttgcttac cgccagaact tgagcagcgg agggaagcaa ttcagcttct
    15781 ggatatcctg agaggtatcc gtcacgatga tccgcaatat caagaggtta gccgtcgatt
    15841 gtcattactg gaattgaagc tgcgacaagc tggactgagt accgattttt tacgcggcga
    15901 ttatgctgac aagttgttga ataaaatcaa cgataactag tggagtatgt atgtatcgca
    15961 ttggtgagct ggcaaaaatg gcggaagtaa ctcccgacac gattcgttat tacgaaaaac
    16021 agcagatgat ggagcatgaa gtgcgtactg aaggtgggtt tcgcctgtat accgaaagcg
    16081 atctccagcg attgaaattt atccgccatg ccagacaact aggtttcagt ctagagtcga
    16141 tccgcgagtt gctgtcgatc cgtatcgatc ctgaacacca tacctgtcag gagtcaaaag
    16201 gcattgtgca ggaaagattg caggaagtcg aagcacggat agccgagttg cagagtatgc
    16261 agcgttcctt gcaacgcctt aacgatgcct gttgtgggac cgcccatagc agtgtttatt
    16321 gctcgattct tgaagctctt gaacaagggg cgagtggcgt taagagtggc tgttgatttt
    16381 ttgcactgac aggattacac tcgcgccgtt aaataaccaa ctggagtttt tatgagccga
    16441 tatcagcata ctaaagggca gataaaggat aatgcgatag aagcattact acatgatccc
    16501 ttattccgac agcgagtaga gaaaaataag aaagggaaag gcagttacat gcgaaaagga
    16561 aaacatggca atcgggggaa ctgggaggcc agtggcaaga aagtaaatca cttttttacc
    16621 actggccttc tgctttcagg cgtttgttaa gagtggttat tctgctcttt cagcaaatca
    16681 cgaatttctg tcagtaatac ttcttcttta gttggtgctg gtgcggctgc tggttcttct
    16741 tttttccgat tcagtttgtt gattagctta atcgccataa agatggcaaa ggccacaatc
    16801 agaaaatcaa agacgttttg aatgaagaca ccgtaatgca tcacaacagc agggatatcc
    16861 ccctgcgcat cgcgtagcgt gacagcaaac tgtttaaagt cgatcccgcc aattaataag
    16921 cccagaggtg gcatgatgat atcggcaacc agtgaagata caatcttccc gaatgccgca
    16981 ccgataatga cacccaccgc caaatccacc acgttcccgc gcatcgcaaa ttcgcgaaat
    17041 tctttaataa tgctcatgtt attctcccta tgaagccgac aaccataagt ctaacaaatg
    17101 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
    17161 taaagcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
    17221 tcgacgtcgg taataaattt tttatctgtg aggaacataa ttacgtgatc gccttgctca
    17281 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
    17341 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
    17401 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
    17461 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
    17521 atcgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
    17581 accatcacct ttttcgcacc catacgtttt gcaagcatgg cggacatgat attggcctcg
    17641 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
    17701 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gtaacttttc cgccagttcg
    17761 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
    17821 gccagccctg caccgatatt accgccacca accagcatga tccgcttata cggtttttcc
    17881 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
    17941 tcatcaccag cttcaacaat ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa
    18001 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
    18061 ccaatcagcg ggccgccata ataggcttta accacagcca ggctgacttt accctcagcg
    18121 aagttcacca cctgcaatgc gccgggatac tcaatcagtc ggtaaatatt atcgataacc
    18181 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
    18241 gcatcgcgca cgtagtctgg tgagcggata cgagcgatgc gattaggggt gttgaaaagc
    18301 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
    18361 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
    18421 acgactcgca ggtcaaattt atcctgcaag gttcgcagac gctcaccgtt ggtatcgaca
    18481 acagtgatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
    18541 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
    18601 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggca gattttgttt acccggttgc
    18661 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
    18721 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
    18781 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttctgggata
    18841 tcgcgatcgc gacgtaacca tttaatatcc ggatggcgac gaatcacacc ggttgctgaa
    18901 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
    18961 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaacg
    19021 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
    19081 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccagaatgtg ttcaccgttt
    19141 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
    19201 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagacgtac agcatcaggg
    19261 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
    19321 cgggaatgat gcgtacgatt aacgcgcagc cacattggcg gacgctggtt attggcttcg
    19381 acgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
    19441 tgcagataac gtgcatcact ggcattaaac tcggctaata actcttcttg ctgacgctgg
    19501 aactggcgta atacaccgtt tatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
    19561 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
    19621 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
    19681 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
    19741 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
    19801 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
    19861 cgttgttttt tcataagtat aaaaatgccc ggcaagaccg ggcttagaag agtggactat
    19921 caggccagac ggttgcccgg aacaaaccat tcccgacgag aattcaggag gtcttgcgcg
    19981 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcacccgtc
    20041 gcaacctgaa tgccttgttt gttggcttca agaatcgttc ctggtgcagc gttggttgcc
    20101 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
    20161 attggccatg gattgaaagc acgaatgcag cgttcaagct gtgctgccga aagtgaccag
    20221 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
    20281 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
    20341 ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt aatcgggcag
    20401 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggtcaca
    20461 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
    20521 ggcagcagtg aaccatgaac gttgatacag ccaagatgcg gcatctccag cactgcttta
    20581 ggcagaatta agccataggc gacgacaacc ataacatcag cctgtagatc ggcgaccagt
    20641 tgctggtttt cttgtggacg cagggaaacc ggttgaaaaa cgggcagacc tttttcctca
    20701 gccagaactt taaccgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
    20761 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
    20821 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
    20881 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaata cgttgttgtt
    20941 tcagcggtga cagataatcc ataaacagtt tgccgaccag gtgatccatc tcatgctgaa
    21001 tacagatggc taacagaccg tctgcttcca attcaaatgg tttaccgtca cggtccaggg
    21061 cgcgaatttt aactttctct gcgcgtggca ctaaagcacg ttgttcaggg atcgacaggc
    21121 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
    21181 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
    21241 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
    21301 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
    21361 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
    21421 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc actgaccaat
    21481 cgcaaagatt gctaaggctg cttatggcag ggagataagg atggtcgata cagaaatttg
    21541 gctgcgttta atgagcatca gcagcttgta cggcgatgat atggtccgta tcgctcactg
    21601 ggtggcaaaa cagtcgcata ttgatgcggt tgtattgcag caaacagggc ttacattgcg
    21661 gcaggcacaa cgctttcttt catttccacg aaagagtatc gaaagctcac tttgttggtt
    21721 ggagcaaccc aaccatcatt taatccctgc ggacagcgaa ttttatcctc ctcaacttct
    21781 ggcgacgaca gattaccccg gcgcactgtt tgttgaagga gaactgcacg cgctgcattc
    21841 atttcagctt gccgtagtgg ggagtcgggc gcattcatgg tatggcgagc gatggggacg
    21901 attattttgc gaaactctgg cgaagcatgg agtgacaatt acgagtggac tggcgcgtgg
    21961 aatcgatggt gtagcgcata aagcagcctt acaggtaaat ggcgtcagca ttgctgtatt
    22021 ggggaatgga cttaatacca ttcatccgcg tcgccatgcc cgactggcta ccagtttgct
    22081 tgaacatggt ggggcacttg tctcggaatt tcccctcgat gttccacccc ttgcttacaa
    22141 tttcccacga agaaatcgca ttatcagtgg tctaagtaaa ggtgtactgg tggtggaagc
    22201 ggctttgcgc agtggttcgc tggtgacagc acgttgtgcg cttgagcagg ggcgtgaagt
    22261 ttttgccttg ccaggtccaa tagggaatcc gggaagcgaa gggcctcact ggttaataaa
    22321 acaaggtgcg attcttgtga cggaaccgga agaaattctg gaaaacttgc aatttggatt
    22381 gcactggttg ccagacgccc ctgaaaattc attttattca ccagatcagg aagacgtggc
    22441 attgccattt cctgagctcc tggctaacgt aggagatgag gtaacacctg ttgacgtcgt
    22501 cgctgaacgt gccggccaac ctgtgccaga ggtagttact caactactcg aactggagtt
    22561 agcaggatgg atcgcagctg tacccggcgg ctatgtccga ttgaggaggg catgccatgt
    22621 tcgacgtact aatgtatttg tttgaaacct atattcacac tgaagctgag ttgcgtgtgg
    22681 atcaagacaa acttgaacag gatcttaccg acgcaggatt tgatcgagaa gatatctaca
    22741 atgccctgct ttggctggaa aagcttgcgg attatcagga aggactggca gaaccgatgc
    22801 aactggcctc tgatccactc tccatgcgta tttatacccc tgaagagtgt gaaagactgg
    22861 atgccagctg tcgtggtttt ctgcttttcc ttgagcaaat tcaggtgctt aaccttgaaa
    22921 cccgtgaaat ggtgatagag cgagtgctcg cgctggataa cgcagagttc gaactggatg
    22981 atctgaaatg ggtgatcctg atggtgttgt tcaacatccc gggctgcgaa aatgcgtacc
    23041 agcaaatgga agaattactc tttgaagtga atgaaggtat gctgcattaa tcatttcttt
    23101 aattcagcat aagttgttat ggcaaaatca gcactgttca cggtgcgtaa taatgagtcc
    23161 tgcccaaagt gcggggctga actggttatt cgatccggga aacacggtcc gtttcttggc
    23221 tgctcacagt atccggcgtg tgactacgtc cgtcctctga aatcttcagc ggatggacat
    23281 atcgtcaaag ttctggaggg gcaggtttgc cctgcatgtg gcgcaaatct ggtattacgc
    23341 cagggacgct ttggtatgtt tattggttgt agtaactacc ctgaatgcga acataccgaa
    23401 cttatcgata aaccggacga aacagcaatt acatgccccc aatgtcggac gggccatctg
    23461 gtccagcgcc gctcccgtta tggcaaaaca tttcactcct gtgatcgcta cccggagtgt
    23521 caatttgcca ttaacttcaa acccatagct ggagaatgcc ctgagtgtca ttatccgcta
    23581 ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt gtgccagtaa acaatgtgga
    23641 aagccggttt cggcggaata ataacgtgaa taataacctg caaggagacg ctatcgcagc
    23701 tgcgatagat gtcctcaatg aagaacgtgt catcgcctat ccaacggaag ccgttttcgg
    23761 cgtcgggtgc gatcctgata gcgaaacagc agtgatgcga ctgttggagt taaaacagcg
    23821 tccggttgat aaggggctga ttttaatcgc agcaaattac gagcagctta aaccctatat
    23881 tgatgacacc atgttgactg acgtgcagcg tgaaaccatt ttttcccgct ggccaggtcc
    23941 tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc tggttgacgg gccgctttga
    24001 ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc aagcttatgg
    24061 taaaccgctg gtttctacca gtgccaactt gagtggattg ccaccttgtc gaacagtaga
    24121 cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa cgggggggcg
    24181 tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac aggggtaaca
    24241 taatggaaac ctatgctgtt tttggtaatc cgatagccca cagcaaatcg ccattcattc
    24301 atcagcaatt tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg ttggcaccca
    24361 tcaatgattt catcaacaca ctgaacgctt tctttagtgc tggtggtaaa ggtgcgaatg
    24421 tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga tgagcttact gaacgggcag
    24481 cgttggctgg tgctgttaat accctcatgc ggttagaaga tggacgcctg ctgggtgaca
    24541 ataccgatgg tgtaggcttg ttaagcgatc tggaacgtct gtcttttatc cgccctggtt
    24601 tacgtattct gcttatcggc gctggtggag catctcgcgg cgtactactg ccactccttt
    24661 ccttggactg tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa gagttggcta
    24721 aattgtttgc gcacactggc agtattcagg cgttgggtat ggacgaactg gaaggtcatg
    24781 agtttgatct cattattaat gccacatcca gtggcatcag tggtgatatt ccggcgatcc
    24841 cgtcatcgct cattcatcca ggcatttatt gctatgacat gttctatcag aaaggaaaaa
    24901 ctccttttct ggcatggtgt gagcagcgag gctcaaagcg taatgctgat ggtttaggaa
    24961 tgctggtggc acaggcggct catgcctttc ttctctggca cggtgttctg cctgatgtag
    25021 aaccggtcat caaactattg caacaggagt tgtcagcgtg aatcaggcca tccagtttcc
    25081 ggacagggaa gagtgggacg agaataaaaa atgtgtatgt tttcccgctc tcgtgaatgg
    25141 tatgcaactg acatgcgcga tctctggcga tagcctggcg tatcgcttta ctggagatac
    25201 gccagaacag tggttagcga gttttcgtca gcatcgctgg gacctggaag aagaagcgga
    25261 aaacttaatt caggaacaaa gtgaagatga tcaaggctgg gtctggttac cctgatccag
    25321 atattcgtcc ttccatttca cgtaattatt cgcggaatag cgtaacccag ccttctcttc
    25381 atcacttaac gggcggatct gtttgacggg gctaccgaga tacagatatc cgctctccag
    25441 ccgtttattt tgtgggacaa gactacccgc accaatcatc acatcatctt ctactattgc
    25501 gccatcaagt aaaattgagc ccatctcaac caaaactcga ttgccaatgg tgcagccgtg
    25561 gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt tgccatctgg
    25621 gttgtacgaa gatttatgag tgacatgcaa catactgcca tcctggatat tggtgcgtgc
    25681 tccgatctgt acataatgta catctccacg aatcacaacg agcggccaga tccccacatc
    25741 atcagccaga cgaacgtcac caattacgac actgctatcg tcgatcatta cgcgctgacc
    25801 gatttgtgga aaaagatcgc ggtatgggcg taaaacatca gacatactta cctcaacaat
    25861 aaatgattta ctaatgactt tgggtacatt attggccttg tgcaagtcgt ttagtatgca
    25921 aaaaagcacc gttttgtgtg cgaatgcagc aaaaagggtg aaaaaacaac aaacagaaaa
    25981 aaagatcaaa aaagtacttg tgcaaaaaat tgggatccct ataatgcgcc tccgttgaga
    26041 cgacaacgtg aaacacttca caagatggtc gtaacaacga agagaaaaaa tcctgaaatt
    26101 cagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag tgagctgaaa
    26161 gccgcgtcgc aactgctctt taacaattta tcagacaatc tgtgtgggca ctcgaagata
    26221 cggattctta acgtcgcaag acgaaaaatg aataccaagt ctcaagagtg aacacgtaat
    26281 tcattacgaa gtttaattct ttgagcatca aacttttaaa ttgaagagtt tgatcatggc
    26341 tcagattgaa cgctggcggc aggcctaaca catgcaagtc gaacggtaac aggaagaagc
    26401 ttgct
//
LOCUS       DAZUGK010000045        26297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZUGK010000045 DAZUGK010000000
VERSION     DAZUGK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.045"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHY5W_001680"
     CDS             complement(<1..101)
                     /locus_tag="ACHY5W_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446591.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKE"
     gene            complement(229..2190)
                     /gene="topB"
                     /locus_tag="ACHY5W_001681"
     CDS             complement(229..2190)
                     /gene="topB"
                     /locus_tag="ACHY5W_001681"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4446592.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLNICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2195..3238)
                     /gene="selD"
                     /locus_tag="ACHY5W_001682"
     CDS             complement(2195..3238)
                     /gene="selD"
                     /locus_tag="ACHY5W_001682"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFS4446593.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3355..3906)
                     /gene="ydjA"
                     /locus_tag="ACHY5W_001683"
     CDS             complement(3355..3906)
                     /gene="ydjA"
                     /locus_tag="ACHY5W_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFS4446594.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAALEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4067..5923
                     /gene="sppA"
                     /locus_tag="ACHY5W_001684"
     CDS             4067..5923
                     /gene="sppA"
                     /locus_tag="ACHY5W_001684"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4446595.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6039..6749
                     /locus_tag="ACHY5W_001685"
     CDS             6039..6749
                     /locus_tag="ACHY5W_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFS4446596.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            6881..7897
                     /gene="ansA"
                     /locus_tag="ACHY5W_001686"
     CDS             6881..7897
                     /gene="ansA"
                     /locus_tag="ACHY5W_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4446597.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7908..8549
                     /gene="pncA"
                     /locus_tag="ACHY5W_001687"
     CDS             7908..8549
                     /gene="pncA"
                     /locus_tag="ACHY5W_001687"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFS4446598.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCLSRG
                     EAVIASQDWHPANHGSFASQHCVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8642..10000)
                     /gene="ydjE"
                     /locus_tag="ACHY5W_001688"
     CDS             complement(8642..10000)
                     /gene="ydjE"
                     /locus_tag="ACHY5W_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446599.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVNVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGAITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(10118..10876)
                     /gene="ydjF"
                     /locus_tag="ACHY5W_001689"
     CDS             complement(10118..10876)
                     /gene="ydjF"
                     /locus_tag="ACHY5W_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4446600.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(11013..11993)
                     /gene="ydjG"
                     /locus_tag="ACHY5W_001690"
     CDS             complement(11013..11993)
                     /gene="ydjG"
                     /locus_tag="ACHY5W_001690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFS4446601.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(12003..12950)
                     /gene="ydjH"
                     /locus_tag="ACHY5W_001691"
     CDS             complement(12003..12950)
                     /gene="ydjH"
                     /locus_tag="ACHY5W_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS4446602.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLVSIFNSPLLDGKALTEIFTQA
                     KARQMIICVDMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGEMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12955..13791)
                     /gene="ydjI"
                     /locus_tag="ACHY5W_001692"
     CDS             complement(12955..13791)
                     /gene="ydjI"
                     /locus_tag="ACHY5W_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4446603.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(13812..14855)
                     /gene="ydjJ"
                     /locus_tag="ACHY5W_001693"
     CDS             complement(13812..14855)
                     /gene="ydjJ"
                     /locus_tag="ACHY5W_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFS4446604.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14872..16251)
                     /gene="ydjK"
                     /locus_tag="ACHY5W_001694"
     CDS             complement(14872..16251)
                     /gene="ydjK"
                     /locus_tag="ACHY5W_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446605.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(16278..17354)
                     /gene="ydjL"
                     /locus_tag="ACHY5W_001695"
     CDS             complement(16278..17354)
                     /gene="ydjL"
                     /locus_tag="ACHY5W_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS4446606.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(17724..17996)
                     /gene="yeaC"
                     /locus_tag="ACHY5W_001696"
     CDS             complement(17724..17996)
                     /gene="yeaC"
                     /locus_tag="ACHY5W_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFS4446607.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFE"
     gene            complement(18038..18451)
                     /gene="msrB"
                     /locus_tag="ACHY5W_001697"
     CDS             complement(18038..18451)
                     /gene="msrB"
                     /locus_tag="ACHY5W_001697"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4446608.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGKEING"
     gene            18793..19788
                     /gene="gapA"
                     /locus_tag="ACHY5W_001698"
     CDS             18793..19788
                     /gene="gapA"
                     /locus_tag="ACHY5W_001698"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4446609.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19872..20756
                     /gene="yeaD"
                     /locus_tag="ACHY5W_001699"
     CDS             19872..20756
                     /gene="yeaD"
                     /locus_tag="ACHY5W_001699"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFS4446610.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGIAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(20807..21661)
                     /gene="yeaE"
                     /locus_tag="ACHY5W_001700"
     CDS             complement(20807..21661)
                     /gene="yeaE"
                     /locus_tag="ACHY5W_001700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFS4446611.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGRFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(21751..22497)
                     /gene="mipA"
                     /locus_tag="ACHY5W_001701"
     CDS             complement(21751..22497)
                     /gene="mipA"
                     /locus_tag="ACHY5W_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFS4446612.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22933..24867
                     /gene="yeaG"
                     /locus_tag="ACHY5W_001702"
     CDS             22933..24867
                     /gene="yeaG"
                     /locus_tag="ACHY5W_001702"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFS4446613.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24980..26263
                     /gene="yeaH"
                     /locus_tag="ACHY5W_001703"
     CDS             24980..26263
                     /gene="yeaH"
                     /locus_tag="ACHY5W_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFS4446614.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         6774 a         6766 c         6152 g         6605 t
ORIGIN      
        1 ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac
       61 ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca
      121 tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc
      181 gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt acgctatcgc
      241 cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
      301 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
      361 cggagtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
      421 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
      481 catgtccggt cgcgtagcca tttctggcag cgaatggaat aacgcttttc cggcgtcggt
      541 ggagtggata tagcgccctt ttttggtcag gaaaccacgc ttgaacaaca gttcaataat
      601 cccggcacgg gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
      661 tttgtcctgc acaaagcgcg cgatcccggt cattgccgaa agcagtgttg catcggtaaa
      721 atggcgcggc ggctgggttt gccgctctac cacttcacct ttttcacaca gcaactcatc
      781 gcctttcgcc accacaggca atggcgtacc gtcgttttct tcatcgcgct ctttgctgcc
      841 taacagcgtg cgccagcctg cttcagcaag aaaacgcgct ttagcgacaa atttgccttt
      901 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggcaga actgcatcaa
      961 atactgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga
     1021 actccgtgcg gtcggaatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
     1081 gttgcgtata tctggatcta ccactggctg cggcaacaga tccggtgcat gaacgctgat
     1141 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcaga
     1201 acgcggataa gtgattagct tgtgcgtttc gtacagtttc tggcagatat taagcacgtt
     1261 ctgcgcgctc agaccaaaac gttttgccgc ttcaatctgc aacgccgaaa gcgaaaaagg
     1321 cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgacaatag ccggttgacc
     1381 actaatacgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
     1441 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
     1501 tgtcacgata tgtgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
     1561 atcgcggcgc accaccagcc cgagcacggg tgtctgtacg cgtcccacgg aaagtacgcc
     1621 ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatattga tgccgtacag
     1681 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacaat ggcacaaact cactgttgga
     1741 ccgaagacgg tcgattgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
     1801 ctgcacctgc tggcgctttt ccggtgccag ttgcagatag tccagcactt catccaccag
     1861 caattgccct tcacgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaaaaa
     1921 gcgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt gccacttttc
     1981 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
     2041 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
     2101 gataaagcca tcgcctttcc ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
     2161 actcggtttt tcggcaataa acaaccgcat tgaattaacg aatctcaacc atggcacgac
     2221 cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
     2281 tagctttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg ccagaagttt
     2341 gcggatcgca cagcaaatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
     2401 tggcaaagtt acgttcggtg ccgccaggca ctgcacccaa cttaatgtac tcttcaaccc
     2461 ccggcaattt cgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
     2521 tttcgctcaa atggcccagc agaccaaaac cagtaacgtc ggtcatcgct tttacgcctt
     2581 caatgttggc aaaggacgcg cctgcgatgt tcatccggca catcacttcc gtcgccagtc
     2641 cctgatgttc tggtttcagc agtgattttt tctcagccgt ggtaagaacg ccgatcccca
     2701 gcggtttcgt caggaacagt ttacatccgg cttgtgcggt actgtttttc ttcacccgct
     2761 cggtcggtac gatccccgtc accgccaaac caaaaatcgg ctccggcgca tcgatggagt
     2821 gaccgccagc cagcgcaata cccgcctgac gacatgcata gcgtccacct tcggtcactt
     2881 cgcgggcaat ttccggggaa agtttattaa tcggccagcc gaggatcgca atcgccataa
     2941 tcggcttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
     3001 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
     3061 cattgcccag atcgtacacc gccgcatcgt cgcgggtttc attacccaca agcaaattcg
     3121 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
     3181 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
     3241 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
     3301 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
     3361 ataagttaca aacggcgtcg ggtccgggac gttaatcgac gtagatgctt tcagctgcgg
     3421 cgtaccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
     3481 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaacc cctgggcaac
     3541 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt cccatcgcgg
     3601 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
     3661 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
     3721 cagtgcggcg ctgaaacgct cgcgtccttc cccttcaatc acaaaaaaat gccacggttg
     3781 catggactta tggtccggcg cgcgcatacc cgcacgcagg atgttttgca gttgttcacc
     3841 cgttggtgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
     3901 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
     3961 tgttacagca cagtccgcaa ttcctgctga caagtgccgg ttgggtcatt acgataacca
     4021 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
     4081 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
     4141 ggtacttaac ctgttcttta tttttctcgt actggttggt gtggggatct ggatgcaggt
     4201 cagtggtggt gattcgaaag aaacggccag tcgtggcgca ctgctgctgg acatttctgg
     4261 tgtgatcgtc gataaaccag acagttctca gcggtttagt aaattaagcc gccagctgct
     4321 tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca acaccattcg
     4381 ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg
     4441 cggcgaccag ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
     4501 gaaaccggtt tatgccgttg gcgagaacta cagccagggg caatattatc tcgccagttt
     4561 cgccaataaa atttggctgt caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa
     4621 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
     4681 gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gacatgtcac cggcagcccg
     4741 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
     4801 taaccggcag atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgagggttt
     4861 aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg tcgatgcact
     4921 ggcatcgagt gcggaaatcg aaaaagcact gaccaaagag ttcggctgga gtaagactga
     4981 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
     5041 tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg aaactcaggg
     5101 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
     5161 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
     5221 tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat cgatgggcgg
     5281 catggcggca tctggtggtt actggatttc cacgccagct aattacattg tggctaaccc
     5341 cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag aaaatagtct
     5401 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcgg atgtttctat
     5461 caccaaggca ctgccgccgg aagcgcagca gatgatgcag ttaagcattg agaatggcta
     5521 taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc agattgataa
     5581 aatcgcccag ggccacgtct ggaccggtca ggacgcaaaa gctaacgggc tggtcgatag
     5641 tctcggggat ttcgatgatg cggttgccaa agcagcagag ctggcaaaag tgaaacagtg
     5701 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaacatgtc
     5761 tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg caccgctggc
     5821 ctcggtagcc tctaccgtta aaagtgaaag tgacaagctg gccgcgttta acgacccaca
     5881 aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taaatcctgc aaaaatgtgg
     5941 ccgatatgct gtcggccaca ctcctgaatc ggactgtctt tcactccctc tctccactac
     6001 attcccttgc tcatttagca tctgatatgg agtcatcagt gaacaaggcc ccctctttaa
     6061 ttgccgctat cgtcctcgga ctggggatta gcgcctgtgg ttattttgtt ggcgatggtg
     6121 ttaaacattt aaaaaccaac aaccgttatg tcaatgtgcg cgggctttct gaaaaagaag
     6181 ttcgcgcaga tacggcggaa ttaacaattg cgattaactt taagggtaat gttcccggcg
     6241 aactgttccc gaaactggaa gaagcgcaga aaaaaattgt tgccgaactc aatgctcagg
     6301 ggattaacga gaaagagata attctgggcc aatggacaag caagcgaaca gattcctttt
     6361 atctgaaaga tgatcccaca atgccacgct ataacgcaga cgggtcagtg acgataaaaa
     6421 cccataatgt tgctgctgtc gaaaaagtcg tcgcgaaatt aaacgaactg caagtcgcta
     6481 cggatggcgc tatcgctgag agcaaagtcg cctatcgctt taatggtatt ggcgcactgc
     6541 gcgctgagat gattgctgcc gccaccaaag atgcacgcaa cgccgcacta caatttgcaa
     6601 cggactcagg tagccaggtg gggtcaatta gcgatgcctc acaaggcgta ttccagatct
     6661 ttgccagtgg cagtgatgaa gatgatccta cggcgattaa taaaacggta cgcgtagtca
     6721 cgaccgtaac ctatgcgttg caggattaac cacttttctc cggcgggaca ctaatccctc
     6781 cgtcctgccg gacttttctc atatccacag agtatccagg tgcgctcgct gcccctcacg
     6841 tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta
     6901 cgttgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt atataccggt
     6961 gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc
     7021 agatttcacc attcatgaat atacgccgct gatggattct tcagatatga cgccggaaga
     7081 ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat
     7141 tctgcacggc accgacacga tggcatatac cgcctctgcg ctgtcgttca tgctcgagaa
     7201 tctcggtaaa ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga
     7261 cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt
     7321 aacgctcttt ttcaataacc gattgtatcg cggcaaccgc actaccaaag cccatgccga
     7381 tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat
     7441 tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat
     7501 caccccacaa ccaattggcg tagtgacgat ttatccgggg atttctgctg acgtcgtgcg
     7561 caattttctg cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc
     7621 gccacaaaac aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt
     7681 ggtcaacctg acacaatgta tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa
     7741 cgccctcgcc catgctggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac
     7801 caaactgcat tacctgctta gccaggaact ggatactgaa accattcgca aggccatgag
     7861 ccaaaacctg cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg
     7921 ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg
     7981 aaggtgacag tacggtggat gtcgctaacc gcctgattga ctggtgcctg tcgcgcggtg
     8041 aagcggttat cgccagtcag gactggcacc cagcgaatca cggcagtttc gccagtcagc
     8101 actgtgtaga gccttatacg ccaggtcaac tcgacggttt gccacaaacc ttctggccag
     8161 atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga
     8221 tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg
     8281 ataacggccg tcggcagaaa acctcgcttg atgactggtt acgcgatcat gaaatcgatg
     8341 aattgatcgt tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt
     8401 tacagttagg ttataaggtg aacgtgatta ccgatggttg tcgtggagtg aatatccagc
     8461 cacaggacag tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc
     8521 tggcagactg ggaagagacg caggggtaat tttacgctgg cctacaattc tgtactggca
     8581 ttgtaggcca aataaaacac gtcagtggca catctggcaa ttgatgccat caacgaaaga
     8641 tttaattcac ctcagaaatc tcttccaacg acaccttccg cgtttcgatg ccaaaaatgg
     8701 agagaaccag cgcacagagc aataacatga caccaagtac cataaacacc gtgatcgccc
     8761 cataatgtgt taataatgcc gcaacgccat agggcgtgaa gactgcgacg atccgtccga
     8821 cggcattaac gaaacccgaa ccgcgcaggc gtaaatgcgt tggccaaagc tccgggatat
     8881 aaaccgccga cgcgaagcaa acatacatgt ataaaaagaa gatcatcacc aggccataga
     8941 ttaaaatcgc ccactctgta gtctgaattg aatagatata gcctaacacg gcaataataa
     9001 taagtaaggt ggagccaaat aaccgacgag gaaaatgatc aataattaat gccgcaataa
     9061 atattcctac cggagcgcca atcataataa cagcggtcat taatattgat ttatcgacat
     9121 caatgccgga gttaacaaat atggtcggta tccatacggt gatggtataa agtgaaatgt
     9181 tcattgcaat taaaacagta atcgcgacta atgtacgtcg taacatttca cctttaaaca
     9241 ggagccagaa agtacccttg attacgttaa ccttgctgtt gctctgatac gaagtaagcg
     9301 ggggtaaacg aatactcttc tctctttcaa tttgctgctc tacttcacga agttggcttt
     9361 ctgcaccggc gatttgccct ttccctgcca gccatcgtgg cgactcgata aagtatttac
     9421 ctgagagaaa ccaggctaac agtatgccaa taccacccaa cagaaacatt atccgccaac
     9481 taaaaaaagc gataaccacc acacctatcg ccgcagacag catgggcgac cagttaccaa
     9541 caaatgagag ccgcgcggac cattttccac gcaccgtcgc ggggataaac tccgtaaatg
     9601 aggcatagcc aaccataatc agcgccccca ttcctgttcc catcaggaag cgaaagaaga
     9661 tgagccagta catatcaggg acaaaagcgg cccctgttgc agcaataccg acgatgagaa
     9721 gatttatgcg aaacgccctg cgccgcccaa agtaatcacc gatgaaccca ccggtaagag
     9781 agccgatgaa ataaccaaac attaatgccg aggtaaaggc ggcattgagg aaattatttg
     9841 accatccatt gcttaccagc tttgctaaga cgacattacc ggagtaactc aaaaaccctg
     9901 ttaataacag actaaagctt ataataccaa atatacgata atgaaaccgg gccaaaggca
     9961 agcggtccag tcttgcgcca atttgatcat attgttccat tgggtgcctc ggatttgcta
    10021 aaaaaggtag caattgcggc aaattcactt gccgcatctt tatcatcatt ttatttccag
    10081 gcacaggaac aaataatatt gacaacgtgt gcttaaatta ccacaccagc tggatattat
    10141 tgtctttgca aaatgctatc cactctgcac ccggtgattt atcagttatt atgtaattaa
    10201 tatgactaaa gtcagctaac tggacaaaag ctttgcgatc aaacttagag tgatcaacca
    10261 ataacgcaac ttctgtcgcc tgacggatca ttgtcttttt gatttcagct tccgcttcat
    10321 tagagtccag cgcgccgctg ttaatatcaa gacctttaca actcattacc atgatatcga
    10381 catgatagcg gctgatgatc tctttagtaa ttcttccttg cagtgaaagt gtatttttgt
    10441 ttagttcccc acccgttgaa acgactttaa tttctgactg agccagaaca tgaatcgctt
    10501 ccgccgagtt ggttagcaac gtcaggtcac tacggtcctg taacaatttg agcaattcca
    10561 ttacggtact actggaatcg gctgccatgg tggttttatt gtcgataaag ggtagtgcct
    10621 tgcgtgcaat aagctgcttc tcttcataaa acgatgaagc gcgcttataa aaatggatat
    10681 tctccgtcaa catcgctgta tttaaaacag caccaccata ggttctggtc aaaaagccct
    10741 catcttccag cttctcaaga tcgcggcgaa tggtttcttc ggttacctga aaaatcccac
    10801 tcaaatttga gactgtcacc tttttatcgt tggcaaccat ttgcttaatt gcctgaatcc
    10861 tgtcttttgc cgccacgatt acacccctgt atctttttac atcacattag cacgattatc
    10921 gcataaccga tgtttacttt caaaataacc tgtttgaatc acagattttc atcacagttt
    10981 tcacagaaac agaggtgaat cgtgttgagt atttaacgct ccagggcctc tgccatttcc
    11041 ctcatcaatg ttgcgtctgc atccgataag ttgatattca gtgccgcgac attttcgcgt
    11101 acctgttccg gtgcagtagc cccactaaga atggagatta aatcactctg ttttaatatc
    11161 cacgccagtg ccagagtggg aattgtgcac tgataacgag cacaaagtgg ctgccactgt
    11221 tcaagcatat caatcacttt cagcatgttt tcacgctgga accagacttt atttgcccga
    11281 gcgccgcccg gaacgtaatc acgagtgatg gtgccggtca acaatccctg ctctagcggg
    11341 gaataaacct gaaccacaat gccattatca cgacatagcg gcagcagttc gttttccatt
    11401 gcccggtcga ggatactgta tttcgcctga ataatatcca gttcaccata ttgcagatac
    11461 tcgcggatat ggtcagcatc gacgttagca gcgcctatag cgcgaatttt cccttcagcc
    11521 tttaactcat taagcactgc gacagtttca gcgatcggcg tataaaatgg cggcaccgac
    11581 tgccagtgcg tcatgtagat atcgatgtaa tcaataccca gacgttgcaa gctggcttct
    11641 acctcttcgc ggatagattc cggggaaagg tttttataca actgccgatc gccaactttg
    11701 ttgaataaac ttccttttcg ttcccagaca atgccgcatt tggtttctac tacaacctgt
    11761 tcacggggca gtttttttaa cgcctgaccg acgataactt cactattgcc aaagttatat
    11821 cctggtgcag tatcaatcag attaatgccg caacgatggg cttcaagaat cgtatcaata
    11881 catatttgcc gatcgagatc gccattccat gcaggaccgc cgccaatggc ccatgtcccc
    11941 aaccccattc gcgaaagcgt aatatccgtt gtgcctaaag gtatcttttt catttgcctt
    12001 cattatcctt cgtattcttc cagcaattgt tcgaccagct ttctgttttt tacgccggtg
    12061 gtggcaccga cgcttagaac cgagatagcc gccgttgcat tggcaaagcg tgcgcattca
    12121 cgcagatttt tgccttctaa cagtgccgca ataaaacctg aagcaaagtt atcgcccgcg
    12181 ccaatggtgt cgatggcggt tattcctgcg accgccggca ccttcatcgt catctcacca
    12241 cgcttgataa agcagccgtc tttacccgtt ttaatcacca ccgtttttac gccgcacgca
    12301 agaaagcagt cagcaatttc atccagtgtc tctttcccgg tgagtaattt tgcctcggca
    12361 taattaggaa acagataatc gacataactc aatgcttcgc aaatatcatc cagcgtttca
    12421 ttcaaccgcg gtttgatcat atcgacacag ataatcatct gccgggcttt agcttgcgta
    12481 aaaatttctg ttagcgcttt accatccaat agtggactgt tgaaaatact gaccagtgat
    12541 aataattttg cctgggaaaa ccgagcaaaa tcaacatcgt caatattcaa tttccacaga
    12601 ctgccgttac ggtttgtgac aaacgtccgc tcgccatcct ccgtcaccag tccaacatta
    12661 atagaggtat ctatgttgac gtcctgcttc aggctttgaa tatcaatatt ctctttgcgg
    12721 caatggtcga gaataaactg ccctgcggca tctttaccaa tacggctcat taatgctgta
    12781 cgatggccca gacgagaaat aattgttgct tcatttattg catcaccccc ggtggtcatt
    12841 gcgattcttt caagaggata agaatccaca tcaaagatat ttttactgac cggttgcaat
    12901 ggaatatcaa caatagcggc acctatacaa ataacgtcga gattatccat atcattattc
    12961 cgctttgcca tcagaaccga acagtttaat tttttccaat gcccgttctt ttacagcttt
    13021 acgtacttca cgttctaaat gcaggaaagg ctgatcctgg ttttctttca ctgcgaccat
    13081 tgcggcctga cagagctccg tatggatgtt gattttcgcg atacccaacg aaattgcagt
    13141 tttaatgtcg gcatcactga tccccgatgc gccatgcaaa accagcggaa cagacacggc
    13201 atcgcgtacg cgtttgacga cctcaaagtt caattgtggc tctgacgtat aaaccccatg
    13261 ctggttgccg atggcgacag ccaatgagtc acagcccgta cgttcaacaa attcagccgc
    13321 ctggtcagga tcggtatagt gatagcccgc cagcgcttcc tcataaacgg tttcattacc
    13381 gacatgccct aattccgcct ctaccggaat acccagcgga tggaagaaat cgacagcctc
    13441 tttggttaag cgaatatttt cttcgaaatc aaatgcggaa gcatcacgca ttaatgaatt
    13501 cataccatga gtccaggcgt tatgaataat ctccatactc cgaccatgat cccaatgagt
    13561 tattaccggc acagttgctt ttcgtgccat tgataccatc atgtgagaga aatcttcaaa
    13621 tgaggtgttg ccgacaaaac ctgtaccaaa agaaataata accggtgatt tcgcttcttc
    13681 ggctgcgtcg ataacgccca tcaacatttc tgcattccat acattaaaat gggcaatagc
    13741 ataacgttta tttgtggcat cgttttccca atatctgata tctgcgagca tgttctattc
    13801 tcccgtatta attaatcgct aatcttaata acgcctttaa taatgtcgcg tttattgtta
    13861 actgactctt caaatgcctg ttgtacatcc cgataatcgt aaatatgcgt caccatcgat
    13921 ttcacatcga atcgccctga agaaatagct tcaatcgtga ccggataacg attggcgtag
    13981 cggaataccg tctggatagt aacttcgcga tttattttga ggaaattgat tgccgaatcg
    14041 ccgggtacag taccaacaat cataatttta ccgccgcgca ttaccagata aggtgcctgt
    14101 ttaacggtga ccgcagaacc cgctgtttcg aaaacaatat ctgcgcccat gtcttcggta
    14161 aattgctgac agcgtgcaat agtgtcttct tttgcgccgt taataaccac tgtcgcacca
    14221 agctgttccg ccattgccag acgtttttcc agcacatcaa cgacggcaat ttccgttgct
    14281 cccaggcatt tgcacgcttg caacgtcatc aaaccaatac aacccgctcc cagaataatt
    14341 atcttcttac ccggtttaac atctgccagc atcgcggcat gcatcccgac tgcggcaggc
    14401 tccaccagcg ccccttccat cgtgtccata ttgtcgggca gtttgtaagt aaagctctcc
    14461 ggatgacaca gatagtgcgt taatgcgccg cggtagttgg gttgtgtcgc cataaaatca
    14521 acgtctgggc agatgttata tttgccttcc agacagtaac gacagtgacc gcaaggaacg
    14581 ccaggttcga tatttacccg atcccccggt ttaaatttgc gcacgcggct tcccacagcc
    14641 acaaccgtcc cggcgcattc atgacccagg ccaatttctt gatttgggtc tttaggcgga
    14701 ataaacggac ctgattcaaa accatgtaca tctgaaccac aaataccgac atattctact
    14761 ttaatcaaaa cttcatcttc tttaggcact ggtatttctg ctgaaataat tttcattgtg
    14821 cccggcacct gcaatattgc ttttgaattt ttcattttga atccacccgt attatttatt
    14881 ggctactgca tcaatactta aactttcaac ggagacacct ttggtttcga ttccaatggt
    14941 ggcgatagca attgctacaa taattgaaac cgcccccaga agaataaaga ctcccgtaac
    15001 gccataacta ctgagcagca ctgcaacggc ataaggtgcg gcaataccac tgattcgccc
    15061 taccgcattc gccagaccgg agccacggag tttggcctct gtcggccaga tctcagggac
    15121 atacactgcc gaggcatagc aaacgtacat atagacaaaa gtaatcagga agaaaccaat
    15181 caacgttatg agcaacatac tggtttgcag tgaatagata tatccgagca ccgcaatcag
    15241 gattaatagc cccaccccca ttgttttacg cggaatttta tccatcacca gcatggcaat
    15301 aaaaatgcca aatggcgcgc caaacatact catggtattt aaaacaatcg agtcttttaa
    15361 attaatcccc tgggtcatga atatcgttgg caaccagtta attagtgtgt actgcacaac
    15421 gttcatggca atcagcacac aagagcctaa tatcacgcgt ttcagtaata ctcctgtcag
    15481 taaggctgaa tacggcaccg cttgtggtgc tttaccgtca tcagcaataa ccacgggcgg
    15541 caaaggttta cctgtctggc gtatgacgcc ttcttctata ctccgcatga ctttctcggc
    15601 ttcctgatac cgtccgcgcg attccagcca gcgcggggat tcaggaaagt agcgccaggc
    15661 cagcgccgta gcgataagcg acaatattgc agggataagc agttgtaccc gccagttcca
    15721 ctctgcacta atcagcggcg tgagtcccat cgctatcaat gaacagagcg gatatgacca
    15781 gttgccaata aaggaaaccc gacttgacca cgttccacga tttctaccgg gcatatattc
    15841 ggtgaaacca gcaaacagcg taaccagtaa agctcccagc ccaactccca tcacaaaacg
    15901 gcaggcaata aggaaatcca tattcggtga gaaagcaccg acaaccatcg aggcaatatg
    15961 aatggcctca tagaggatga acgcatttct gcgaccggtt ttgtcgccaa tgatgccgcc
    16021 caccagcgca ccgagaaaca ttccggcggt cgtgattgct gagaatgtgg ctgtggtgga
    16081 attatctgtc cagcccaacg ctttcagctg cgcgaggatc aagccaccaa cggcattact
    16141 ccagcagaca agcaagccaa acgcgacgat ggcaaacatt gatgaatgcc agcggcaatc
    16201 cggtaagcga tccagccgtg caccacaatg cggttttgtt atctgttcca tgcgataacg
    16261 ttccttcagg agagtggtta ttcatcaaag tcgtaagtca tgatcacttt gattgcggtt
    16321 ttatcgacca tcgcatcaaa cccttcgcgc cattgcgaca ggccgatacg atgcgtgatc
    16381 atcggtttga ctttgatagc gccgctggct aatagcctga tagcgttacg ccatgaggtg
    16441 gagtcatagg ccatatgccc aatgatgctt ttgttccagg cggtaatgtc attaatcgag
    16501 aaatcaagag gtttgaagcc cattccaacg cgtaccactt ccccgttcgg gcggagcatt
    16561 tcgatggctt gtttcagtgc gatattggca ccggagcatt caatcaccag tcccagattg
    16621 tctttgccac aaatttgctg gcagcgcgcc accacatctt cggtagaacc atttactact
    16681 gccgtcgcac ccagttcttt tgcaaccggg aagcggaccg ccacatcttc ttgcagacca
    16741 acgacgacga tatttaccgc ccccataatt cgcgccattt gtacggagaa cagcccgagt
    16801 gggccagtgc cgatgacgac cacatcctga ccagggagga atttcgattg ctgcgcgatg
    16861 gatttgtagg cattacagat agggtcaagt acggctgcgt cctcataatc aacaccatca
    16921 gggatttccc acaacgcatg acgatgaatt ttgagaattt caccaggaac cagacaatat
    16981 ttggaaaaac caccgcccca ggtattgtta tccagaccaa ggtttacttt ttctgtacaa
    17041 cacagaaaat caccttgttc acaggccgga caaacgccgc agacgtgacc gctgttatcc
    17101 gacacgacgc gttgccccac tttccagtct ttgacttttt caccaacctg cgcaatacaa
    17161 cctgcaaact catggccgcg gatagagtta aactcatcag aaccgctatc gacattgtag
    17221 tgcttcatgt ctgcgccgca gattgccgcg gctttaattt caatcactac atcttccggg
    17281 ccacaaatgg gttgtggcac atcgatcatc ttgtagccgc caaaggcttt gccaaaccga
    17341 gccagtgctt tcattgcgtc atcctctgtg ttttgttttg cttagatggt catcatactt
    17401 acccacatga tttttgaggt caacattaaa tctgtaaaac acagataatg atctgcgttt
    17461 tacaactcag atcacaatta cgcagaaaat ggctgggtct gttacaggtt gatggaaggc
    17521 ggggcgcaaa aagagcaaat tcgagaggga ttaccggcca aataggtctg agacttaagt
    17581 tcaggagaag tcattcaggt gtgaacgcga cggcaagaga tcggactggg acttagccag
    17641 gtcatggcga gataattgag atgagtcgtg gggtcattgt tggctatccc cttcatagcc
    17701 gcggcaaaaa ggggatgaac cacttactca aacatggcaa taggctttgc tgagatacca
    17761 aaatcttctt ttaactgctg tttgctcttc atgaccatct gaccatttgt gtcaattgtc
    17821 atatgctgtg cttcggtatt atggcgggct tgccatagca tcaccagttg caggcagttc
    17881 tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt tccccagttc aacggcggtc
    17941 gacaaacgct ggtatacctc aggcgtcatg ctgttgataa tgtcatcaag attcataatt
    18001 tttccgctcc tgtggaataa gttgctgaat cgttttttca accgttgatt tctttgccgt
    18061 tttcgccatc ggtaaagcgt aaagaggcag agttaacaca ataacgttcg cccgttggct
    18121 gcggcccgtc ggggaagaca tgccccagat gtgcatcaca gttaccgcaa cgaatttcta
    18181 tgcgctgcat tccatgtgac aagtctttga tataacgaat ggattcttca cttaccggtt
    18241 cgtagaaact gggccagcca cagccggaat catacttggt ttgggaatga aacagcgggg
    18301 catcgcagat caaacagtga tatacgccgt cacgcttgtt atgcagtaaa cgacccgtaa
    18361 atggcggctc tgtcccatga ttctgcgtca cgtaaaactg catctcggac aaattttttt
    18421 tcagttcttc tgccgaaggt ttattagcca tttgctcaca tctcacttta atcgtgctca
    18481 cattacgtga ctgattctaa caaaacatta acaccaactg gcaaaatttt gtcctaaact
    18541 tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa tcacattttt atcgtaattg
    18601 ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag cccaaaggaa gagtgaggcg
    18661 agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg caatgattga cacgattccg
    18721 cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc ttttattcac taacaaatag
    18781 ctggtggaat atatgactat caaagtaggt atcaacggtt ttggccgtat cggtcgcatt
    18841 gttttccgtg ctgctcagaa acgttctgac atcgagatcg ttgcaatcaa cgacctgtta
    18901 gacgctgatt acatggcata catgctgaaa tatgactcca ctcacggccg tttcgacggt
    18961 accgttgaag tgaaagacgg tcatctgatc gttaacggta aaaaaatccg tgttaccgct
    19021 gaacgtgatc cggctaacct gaaatgggac gaagttggtg ttgacgttgt cgctgaagca
    19081 actggtctgt tcctgactga cgaaactgct cgtaaacaca tcaccgctgg tgcgaagaaa
    19141 gtggttatga ctggtccgtc taaagacaac actccgatgt tcgttaaagg cgctaacttc
    19201 gacaaatatg ctggccagga catcgtttcc aacgcttcct gcaccaccaa ctgcctggct
    19261 ccgctggcta aagttatcaa cgataacttc ggcatcatcg aaggtctgat gaccaccgtt
    19321 cacgctacta ccgctactca gaaaaccgtt gatggcccgt ctcacaaaga ctggcgcggc
    19381 ggccgcggcg cttcccagaa catcatcccg tcctctaccg gtgctgctaa agctgtaggt
    19441 aaagtactgc cagaactgaa tggcaaactg actggtatgg cgttccgcgt tccgaccccg
    19501 aacgtatccg tagttgacct gaccgttcgt ctggaaaaag ctgcaactta cgagcagatc
    19561 aaagctgctg ttaaagctgc tgctgaaggc gaaatgaaag gcgttctggg ctacaccgaa
    19621 gatgacgtag tatctaccga tttcaacggc gaagtttgca cttccgtgtt cgatgctaaa
    19681 gctggtatcg ctctgaacga caacttcgtg aaactggtat cctggtacga caacgaaacc
    19741 ggttactcca acaaagttct ggacctgatc gctcacatct ccaaataagt tgagatgaca
    19801 ctgtgatcta aaaagagcga cttcggtcgc tctttttttt acctgataaa atgaagttaa
    19861 aggactgcgt catgattaag aaaatttttg cccttccggt catcgaacaa atctcccctg
    19921 tcctctcccg tcgtaaactg gatgaactgg acctcattgt ggtcgatcat ccccaggtaa
    19981 aagcctcttt tgcattacag ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag
    20041 aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg catcgctatt cgcggtggcg
    20101 taccggtttg ctggccgtgg tttggtccgg cggcacaaca aggcctgcct gcgcacggtt
    20161 ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga agatgctgat ggcgtagcgc
    20221 tgacttttga attgacacaa agcgaagaga cgaaaaaatt ctggccgcac gactttacgc
    20281 tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga tcttgaagca cacggcgaat
    20341 ttgaaaccac ctctgccctg catacctact ttaacgtggg tgatatcgct aaggtaagcg
    20401 tcagtgggct gggcgatcgc ttcattgata aagtaaatga cgcgaaagaa gatgtactga
    20461 ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt
    20521 gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc cgtaggccac cagcatcatc
    20581 tgaacgttgt aggctggaac ccgggaccgg cgctttcagt tagcatgggc gatatgccgg
    20641 atgatggcta caaaacattt gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag
    20701 tgaccaaaga gaaacctgca catctggcgc aatccattcg cgttgcgaaa cgttaatttg
    20761 cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta tcaacatcac accatatcca
    20821 gcgcagtttt tccttttggt gccggatacg ctttgtccag catggctaat tccgctgacg
    20881 aaagttcgac ctcaagcaca gccgcattct gttggacatg ggcaatcgtg gccgcttttg
    20941 gaatcgccat cacaccctga tggctgatta cccacgccaa caatacttgt gccgcgctga
    21001 tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc
    21061 gcccggcctg ggctaacgga ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg
    21121 ggagtagatc gtactcaatc cctcgtgaac cgagatggta aagcacctga ttagtggcac
    21181 actgatttcc ccccggcagc tgccagagtt cctgcatatc agcataatca aggttagaaa
    21241 cgccccagcg gcggattttt ccctgggcga tcaatttttc catcgctgcg acagtctctt
    21301 caaaagcgaa acggccagac cagtgtaata agtagagatc gagataatca gtattgagac
    21361 ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc gccagcattc cacggataga
    21421 ctttagagac gagaaagacg ttatcgcgca gaccggttaa tgcttcccca accacctttt
    21481 cggcaccgcc atcggcatac atttcggcgg tatcaatgag ggttaaaccg agctcaatgc
    21541 ccgcgcgtag tgcagcaact tctgttttgc gctgactggc atcttcgccc atataccatg
    21601 ttccctgccc tatggctggc agtgagacat cgccactaaa ttgaatcatt ttttgttgca
    21661 ttgtttcctc ccaggtattg caccaccgta atgcaaaaca gggcgtgacg ccctgttttt
    21721 atgcacaaaa tgccctggaa agatgcatta tcagaatttg taggtgatcc cggtagaaat
    21781 caggccagtc caggatttat ccaccatcgg gctgtcagta acttcatcag acagacgggt
    21841 gtagcgcgcg gtaccgtaaa cactccagtc gccgaggaag ttgtagctgg cgctcagctc
    21901 caggtaaggg ctccagctgt cgttcgggtt atagccacgc agaccgctgc gagcggactc
    21961 tttgcgcgat acgccataat agtattcgtt ctggttttcg ctgttccact gcacaccaat
    22021 acccggagtc acggtcaggc caccgttggt gtaacgatac aaccaggcca tatcccagac
    22081 gatgccgttg ctgttatcca gggtatcgcc agccagggtg gtacgcaggt aaccgtactg
    22141 ggtaaagtga gcataagaca gaccagccat catggtgctc ttacggtcat ccaggtgacg
    22201 catttggtga tcgccactgt ctttagcttt gaagtaaagc ggcgaccagt aagcggtaat
    22261 tgaaagttta tccgttgcgt cattccacag gtagtaacca ccacctaagc cacggaacca
    22321 gaagttatcg ccttcatagt tgattaccgg tactgggtaa acatcggtat cgtaatcttt
    22381 atatgggtgt tcaacgacac ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc
    22441 tacgcctgca gacgttgcga taagcactcc aagtgccaga agtttgagtt tggtcacaat
    22501 taatcattcc ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa
    22561 cccaaccttt ttacgttttc attttttaaa gtaactgttt aattttcctg acgcggatga
    22621 caccgcgctt acagccaaat gaatttagcg ttactggcga gcctggtctt tacattaatt
    22681 atgcaaaatt tatggatgag ttgttgatat gccattgaaa ttaagaaagc cgtgtacgca
    22741 agttttccat ttgccatcta cgcttaattt tgaaggtgta tcaccgggca cgttgttctc
    22801 atcgtcgata aaatggcatg agagttgctg tgttttagca agagacgtcg ttcagtttac
    22861 ctcttccggg agcctctact attcatatga acggctctta acctgtgcta aaaaacgaaa
    22921 ggacggcata ccatgaatat attcgatcac tatcgccagc gatatgaagc tgccaaggac
    22981 gaagagttca cactgcagga gtttcttacc acttgtcggc aagatcgcag tgcttatgcc
    23041 aacgcggctg agcggctatt gatggctatc ggtgagcctg tcatggtcga tacagcccag
    23101 gaacccagac tttctcgact cttttctaac cgggtcattg cacgttatcc ggcgtttgaa
    23161 gagttttacg gcatggaaga cgcgattgaa cagattgtct cttatctgaa acacgcggct
    23221 caggggctgg aagagaagaa acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa
    23281 tcatcgcttg ctgagcgact gaaatcatta atgcagctcg taccgattta tgtattgagc
    23341 gccaacggtg agcgtagccc ggtcaacgat catccgttct gtcttttcaa tccgcaggaa
    23401 gatgcgcaga ttctggaaaa agagtatggc attcctcgcc gttatctcgg caccatcatg
    23461 tcgccgtggg cggcaaaacg tctgcatgaa tttggtggcg atattactaa gttccgggta
    23521 gtgaaggtct ggccgtccat tctgcaacaa attgctatcg ccaaaacaga gcccggtgat
    23581 gagaacaacc aggacatctc cgcgctggta gggaaagtcg atattcgtaa actcgaacac
    23641 tacgcgcaga atgacccaga cgcctatggc tattccggcg cactgtgccg cgccaaccag
    23701 gggatcatgg aattcgttga gatgtttaaa gcaccgatta aagtgctgca tcccttgtta
    23761 accgccactc aggaaggtaa ctacaacggg acggaaggta tctccgccct gccgttcaac
    23821 gggattattc ttgcccactc gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac
    23881 aacgaagcct tcctcgatcg tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc
    23941 gaagagatca aaatctacga gaaattgctt aatcacagtg aattgactca cgccccatgc
    24001 gcccctggca cgctcgaaac actgtcacgt ttttccattc tttcgcgcct gaaagagcca
    24061 gaaaactcca gcatttattc aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc
    24121 gatcccaaag ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga tgaagggatg
    24181 aacggtctgt cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat
    24241 gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc tggaacagca gatcgagcgc
    24301 gagcagttcc cacaagagca ggcagaacgc tatctggagt tcctgaaagg ttatctgatc
    24361 ccgaaatatg ccgagtttat tggcaaagag atccagacgg cctaccttga atcctattcc
    24421 gaatatgggc aaaacatttt cgaccgttat gttacctacg cggatttctg gattcaggat
    24481 caggagtatc gcgatccgga taccgggcag ctgtttgacc gcgagtctct taacgccgag
    24541 ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa aagatttccg caacgagatt
    24601 gttaacttcg tactgcgcgc cagagccaat aacagcggac gcaatccgaa ctggaccagc
    24661 tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct ccaataccga ggagctgttg
    24721 ccggttatct cgtttaacgc caaaacgtca accgacgagc agaagaaaca cgacgacttt
    24781 gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg
    24841 tatttgcgtg tacgtaaatc gtcttaacaa ccctggcccg gtacacatgc taccgggcct
    24901 acaacgacag cgaaccgtgg gccagagaaa tggcaacaca ggcgtagcat acagttggca
    24961 aatgtagtac ggggggcata tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa
    25021 aagcatggtg aatcgccagc gttttttacg ccgttataaa gcgcaaatta aacagtcgat
    25081 ctccgaggcc attaataagc gttcggtgac tgacgtcgac agcggcgaat ccgtatccat
    25141 tcccacggaa gatattagcg aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg
    25201 cgtacatccg ggcaatgacc atttcgtcca gaacgaccga attgaacgtc cccagggtgg
    25261 cggcggaggt tccggcagtg gtcagggcca ggccagccag gatggtgaag gtcaggatga
    25321 attcgtcttt cagatttcga aagatgagta tcttgatctg ctctttgaag atttggcctt
    25381 accgaatctg aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc atcgggcggg
    25441 ttataccgct aacggcgttc cggccaatat cagcgttgtg cgttcattgc agaactcact
    25501 ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa cttcatgcac tggaagagaa
    25561 tttggccatc atcagcaaca gtgaacctgc gcagctgctg gaagaggaac gtctgcgcaa
    25621 agaaattgca gaattacgtg ccaaaattga acgcgtccct tttattgaca ccttcgattt
    25681 acgttacaag aactacgaga agcggcccga tccctccagc caggcagtga tgttttgcct
    25741 gatggacgtt tccggttcaa tggatcaatc cactaaagat atggctaagc gtttttatat
    25801 tctgctgtat ctgttcctca gcagaacgta taagaacgtg gaagtcgtat acatccgcca
    25861 tcatacccag gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg aaactggcgg
    25921 caccattgtt tccagcgccc tgaaactgat ggatgaggta gtgaaagagc gttataaccc
    25981 ggcacagtgg aatatttacg ctgcacaagc atcggacggc gataactggg ccgatgactc
    26041 tccgctttgc catgaaatcc tggcgaaaaa attattacct gttgttcgtt attacagcta
    26101 tatcgaaatt actcgtcgtg cacatcagac attgtggcga gaatatgagc atctgcaatc
    26161 tactttcgac aactttgcga tgcagcacat ccgcgaccag gatgatattt atccggtgtt
    26221 ccgtgaactg tttcataaac aaaatgcaac agctaaagac taaaactatc agccgggtca
    26281 ttatcgcctg gctgatt
//
LOCUS       DAZUGK010000046        25991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZUGK010000046 DAZUGK010000000
VERSION     DAZUGK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.046"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..467
                     /locus_tag="ACHY5W_001704"
     CDS             105..467
                     /locus_tag="ACHY5W_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446615.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(570..1028)
                     /gene="ybbJ"
                     /locus_tag="ACHY5W_001705"
     CDS             complement(570..1028)
                     /gene="ybbJ"
                     /locus_tag="ACHY5W_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4446616.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1025..1942)
                     /gene="qmcA"
                     /locus_tag="ACHY5W_001706"
     CDS             complement(1025..1942)
                     /gene="qmcA"
                     /locus_tag="ACHY5W_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4446617.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2088..2765
                     /gene="fetA"
                     /locus_tag="ACHY5W_001707"
     CDS             2088..2765
                     /gene="fetA"
                     /locus_tag="ACHY5W_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFS4446618.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2752..3531
                     /gene="fetB"
                     /locus_tag="ACHY5W_001708"
     CDS             2752..3531
                     /gene="fetB"
                     /locus_tag="ACHY5W_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFS4446619.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3594..4448)
                     /gene="cnoX"
                     /locus_tag="ACHY5W_001709"
     CDS             complement(3594..4448)
                     /gene="cnoX"
                     /locus_tag="ACHY5W_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFS4446620.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4509..5318)
                     /gene="ybbO"
                     /locus_tag="ACHY5W_001710"
     CDS             complement(4509..5318)
                     /gene="ybbO"
                     /locus_tag="ACHY5W_001710"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS4446621.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5308..5931)
                     /gene="tesA"
                     /locus_tag="ACHY5W_001711"
     CDS             complement(5308..5931)
                     /gene="tesA"
                     /locus_tag="ACHY5W_001711"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFS4446622.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5902..6588
                     /gene="ybbA"
                     /locus_tag="ACHY5W_001712"
     CDS             5902..6588
                     /gene="ybbA"
                     /locus_tag="ACHY5W_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFS4446623.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6585..8999
                     /gene="ybbP"
                     /locus_tag="ACHY5W_001713"
     CDS             6585..8999
                     /gene="ybbP"
                     /locus_tag="ACHY5W_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFS4446624.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVSESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVILGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLMQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(9216..9371)
                     /gene="dinQ"
                     /locus_tag="ACHY5W_001714"
     CDS             complement(9216..9371)
                     /gene="dinQ"
                     /locus_tag="ACHY5W_001714"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFS4446625.1"
                     /translation="MKKIIVTGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALQE
                     LIRQFIE"
     gene            complement(9711..10805)
                     /gene="mnmH"
                     /locus_tag="ACHY5W_001715"
     CDS             complement(9711..10805)
                     /gene="mnmH"
                     /locus_tag="ACHY5W_001715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFS4446626.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPVEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(10874..11800)
                     /gene="allS"
                     /locus_tag="ACHY5W_001716"
     CDS             complement(10874..11800)
                     /gene="allS"
                     /locus_tag="ACHY5W_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFS4446627.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            12029..12511
                     /gene="allA"
                     /locus_tag="ACHY5W_001717"
     CDS             12029..12511
                     /gene="allA"
                     /locus_tag="ACHY5W_001717"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFS4446628.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            12589..13404
                     /gene="allR"
                     /locus_tag="ACHY5W_001718"
     CDS             12589..13404
                     /gene="allR"
                     /locus_tag="ACHY5W_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFS4446629.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            13494..15275
                     /gene="gcl"
                     /locus_tag="ACHY5W_001719"
     CDS             13494..15275
                     /gene="gcl"
                     /locus_tag="ACHY5W_001719"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFS4446630.1"
                     /translation="MAKMRAVDAAMYVLAKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVTEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAVLTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            15288..16064
                     /gene="hyi"
                     /locus_tag="ACHY5W_001720"
     CDS             15288..16064
                     /gene="hyi"
                     /locus_tag="ACHY5W_001720"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFS4446631.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            16164..17042
                     /gene="glxR"
                     /locus_tag="ACHY5W_001721"
     CDS             16164..17042
                     /gene="glxR"
                     /locus_tag="ACHY5W_001721"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS4446632.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETAHQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKTLALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            17211..18665
                     /gene="ybbW"
                     /locus_tag="ACHY5W_001722"
     CDS             17211..18665
                     /gene="ybbW"
                     /locus_tag="ACHY5W_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFS4446633.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            18725..20086
                     /gene="allB"
                     /locus_tag="ACHY5W_001723"
     CDS             18725..20086
                     /gene="allB"
                     /locus_tag="ACHY5W_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFS4446634.1"
                     /translation="MSFDLTIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            20143..21444
                     /gene="ybbY"
                     /locus_tag="ACHY5W_001724"
     CDS             20143..21444
                     /gene="ybbY"
                     /locus_tag="ACHY5W_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFS4446635.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            21466..22620
                     /gene="glxK"
                     /locus_tag="ACHY5W_001725"
     CDS             21466..22620
                     /gene="glxK"
                     /locus_tag="ACHY5W_001725"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFS4446636.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGIGGSATVDGGMGMAQALGVRFLDAD
                     GQVLVANGGNLARVESIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGAT
                     PEMVEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVL
                     NAVNLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVE
                     VVHQYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(22749..23534)
                     /gene="allE"
                     /locus_tag="ACHY5W_001726"
     CDS             complement(22749..23534)
                     /gene="allE"
                     /locus_tag="ACHY5W_001726"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFS4446637.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(23545..24780)
                     /gene="allC"
                     /locus_tag="ACHY5W_001727"
     CDS             complement(23545..24780)
                     /gene="allC"
                     /locus_tag="ACHY5W_001727"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFS4446638.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGGLAAWL
                     AIDRLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQAIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(24802..25848)
                     /gene="allD"
                     /locus_tag="ACHY5W_001728"
     CDS             complement(24802..25848)
                     /gene="allD"
                     /locus_tag="ACHY5W_001728"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFS4446639.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     DAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSSE
                     LFRQHLSQTMRELNAITPAPGLNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISDA
                     LYNTSYETKNPFAQ"
BASE COUNT         6030 a         6428 c         6909 g         6624 t
ORIGIN      
        1 tctgtttctg gactaaacgt gaagcggttc ctgatgtgtg ctaaaatgcg tttctttgat
       61 aaatctcaat ttttaagagg tttacgccag gcaggaaaat ttaaatgaat gaatttgaga
      121 agatttttaa tgaaatgaat ctggaccggg cattgctacc gatccttttt cgttcgaacc
      181 ggtcgacagt atggaagtat ttaagcggcg atagtacggc tcctgcctct gccatgtcgc
      241 tgattatgct gttgcagttg atccagaaac gaaaccccga tctgttagcc gaatggttaa
      301 cgctttctga tttcaccatt ccgccagaag tttacctgga ccagcctgat tactggaaag
      361 ggtgggtgta tacgcaacac aaggtcaata aaaacgtcct ggaatatcta aaaaaacact
      421 atccggatga agaccaaaaa agtatgagta aaggccggga agagtaattt tgcctctact
      481 cggctatatg ccggatgcgg cgcaagcgcc ttatctggcc tacgattcgg gcatattttg
      541 taggcatgat aagacgcgtc agcgtcgcat caggcgatga ccgcacggat gatcagcgtt
      601 atcccttcta tcgcaatgac ttcaacatgc gtacctgcgc cgagatcctc gctggcgctg
      661 acaggccatg aactgtcacc gacgcgcata tgaccgcgcc cgttgaccag cggagattcc
      721 agcacaaaac gtcggccaat cagctgctgc ccgcgctggt ttaaatgact gtcgctgtgc
      781 ttttgttcgc gcacccgccg cgacaaccat ttccaccaca gccaggcggc gagcagcgtc
      841 aggacggcaa acatcacccc ttgccactcc caacccagcg gcaccagcca gactaccagg
      901 ccggtaatca ccgctgccac gccgctccac aacaaataac catttccgcc cagcatctcg
      961 gctgccagca gcaaaccgcc gagacttagc cagaaaatat gtggatgaac gaccattaac
     1021 tccatcatgg ctgagtccgc ttgttggcgc tgtctttcac cagctcggca atcccggcaa
     1081 tcgaccccat caggctgctg gcctctaatg gcatcatcac tactttgctg ttactggacg
     1141 aaccgatctg ctgtaacgct tcggtgtatt tctgcgctac gaagtagttc accgcctgaa
     1201 tatcaccgga ggcgatggct tcagacacca ttttggtggc gcgggcttct gcttcggcgg
     1261 aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg ttcgccttcc gctttcagga
     1321 tttgcgactg tttttcacct tcggctttga ggatttccgc ctgacggatc ccttccgctt
     1381 cgaggatgta agcgcgtttg gtacgttccg ctttcatctg cgcgttcatt gaagagataa
     1441 gctctgccgg tgggcgcacg tcgcgaattt caatacgggt gactttaatc ccccacgggt
     1501 tggtggcttc atcgacaata cgcagcaggc gtgagttgat gctgtcgcgc tgagagagca
     1561 tttcgtcaag ttccattgaa cccagcacgg tacggatgtt agtcatggtc aggttgatga
     1621 tcgccagctc cagattgctg acttcataag ccgcgcgtgg cgcgtcaatc acctgaataa
     1681 aacagactgc gtcgatggta acgttggcgt tatctttcga gataacttcc tgggaaggga
     1741 tatcgagcac ttgctccatc atattgatct tgcgaccaat gcgatccata aacggcacca
     1801 ccagactgag ccccggctgt aacgttttgg tatagcgacc aaaacgttct accgtccatt
     1861 gatagccctg cggcacgatt ttgacgcccg cgccgacaat gaccagcgcg acaaaaatga
     1921 gaatcgggat aaagataagc atcggaaaaa cctcctgttg taccgtccat aatcagcaaa
     1981 attgctgctt gattaaacaa attatacctg attactgaaa gagagttccc ccttattcct
     2041 gcgaaggata aactgttttt agtaaaaatc agaaaaaggg aacagcgatg caggaaaata
     2101 gtcctttgct tcagctacaa aacgtaggat atctggcggg tgatgcgaag attcttaata
     2161 acatcaattt ttcgctgcgt gctggcgaat ttaagttaat taccggtcct tctggttgtg
     2221 gcaaaagtac gctgctaaaa atagttgctt cattgatcag cccaaccagc ggaacgttac
     2281 tgtttgaagg tgaggatgtc agcacactaa agccagaaat ctaccgccag caagtctctt
     2341 actgcgccca gacaccgacg ctgtttggcg atacggtata cgataatctg atctttccct
     2401 ggcagatccg taaccggcag cctgacccag ccatttttct cgattttctc gaacgcttcg
     2461 ccttgccgga cagcattttg acgaaaaata tcgccgagct atctggtggt gaaaaacaac
     2521 gcatctcact gattcgtaac ctgcaattta tgccgaaggt tttattgctg gatgaaatca
     2581 ccagtgcgct ggatgaaagt aataaacata acgttaatga gatgatccat cgttatgtgc
     2641 gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa agacgaaatt aatcatgcgg
     2701 ataaagtgat tacattgcaa ccgcatgccg gagaaatgca ggaagcacgc tatgaactcg
     2761 cataatatta ctaacgaatc attagcactg gcattaatgc tggtagtggt ggcaatctta
     2821 attagccata aagaaaaact ggcgctggag aaagatattc tctggagcgt cgggcgcgcg
     2881 ataattcagc tgattattgt tggctatgtg ctgaagtata ttttcagcgt ggatgatgcc
     2941 agcctgacat tattgatggt gttatttatc tgctttaatg cggcgtggaa cgcgcaaaaa
     3001 cgcagtaaat atattgctaa agcttttatc tcatcgttta ttgccattac ggtcggagcg
     3061 ggaattaccc tggcggtact gattctctca gggtcgattg aatttatccc gatgcaggtg
     3121 atccctatcg ccgggatgat agccggtaac gccatggtag cggtaggact gtgttacaac
     3181 aatttagggc aacgggtgat tagcgaacag caacagattc aggagaaact gagtcttggc
     3241 gcgacgccga aacaggcttc agcgatattg attcgcgaca gtattcgcgc ggctttaatt
     3301 ccgacggtcg attcagcaaa aacggttggc ttagtgagtt taccaggaat gatgtccggg
     3361 ctgatatttg ccgggattga tccggtgaag gcgattaaat atcagattat ggtgaccttt
     3421 atgctgctct caaccgccag cctgtcgacc attattgcct gctatttaac ctatcgtaaa
     3481 ttttacaatt cgcgccacca gttggtggtg acgcaattga agaagaaata atgcgatgcc
     3541 ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc gatcaacgga atatcaatac
     3601 aacaatgcat acagctggcg gcgatacttc gacgccagtg catcacccgt acccagcgca
     3661 gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg cggcggtgag atctttacgc
     3721 agatgcccga acagcaactc cagcgcctct tcattgcgcc caacctgatg cagttgcagc
     3781 gccagttgcg tcgccagtgc ggcatcttct ggattctccg ccacctgctg ttgcaactgt
     3841 tgaatttccg gcgtatcagc cgcctgcttc agcagttcga tttgcgccac cagcccctgg
     3901 tagcgggtgt cctgatcctg caacggaatg gttttcagca ccgcttccgc atcttcagaa
     3961 cggttcagcg caatcagcgt ttctgccagc agcaggccga tctccccgtt ctgattcgac
     4021 aactgccagg cgtctttcag caatggcagg gcatcggtgt aattgccttc ctgcatcagt
     4081 tgcattgcct gctgcgcttt cagctcttct tcgcgcggca gcactttatc cagcagggcg
     4141 cggatcgcct cttccggttg cggcccctgg aagccatcta ccggttgccc gttctggaac
     4201 agatacacgg tcggaatcgc acgcagacca aactgcgcgg caatcatctg ctccgcgtcg
     4261 cagtccagct tcgccagaat aaattgcccg ttgtactgcg ccgcgaggct ttccagaatt
     4321 ggggttaact gcaaacagtg ctggctacgt tcagaccaaa aatagaacag caccggagtg
     4381 gtcatcgact gttcaagaac ctgttgcagg ttagattcgt taatgttgac aatattttct
     4441 acggacatgg agtcgctctc tgttgtcgat attttctttg acatgggggc ttaagcgcgc
     4501 gcttcaactc acccctgcaa tattttgtcc atcacgcgcc ccggcagcag gcgcttaagc
     4561 accattactg cccaggtcac cagcgttacc ggatagcgca tcttcggctt ctcgctaata
     4621 aaagcatggc gtactttgtc gaccaccgct tccggtccca acgtaaagcg agcagcgatg
     4681 ccgggatttt cgactggttt atcactttgc gtctggttga cgttgtcggt gaagcgagta
     4741 cgaatgggac cgggttcgat caggctgact ttaattccgc tgtggcgtag ctccatgcgc
     4801 agcgcgtctg accacgcctc cagcgcatat ttactggccg cgtaagcgcc acgacccggc
     4861 gtggagatta atcccatcac cgatgatgtc atcacaatac gcccttcacc gtgcggtaac
     4921 atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga aaaagttggc ggaaaactgc
     4981 tgttccatct gcgcacggct gatggtggaa agggggccat acatgccgaa tccggcattg
     5041 ttaaagatcc catacagaca attatcggtc agggcgatca cctcgtcggc tgcgcgatca
     5101 acactttctg gtgaatccag atcgatcaac acgccggtaa atcccatgct gttcatgcgc
     5161 tcaacatcat ccggtttccg gcaacctgcc agcacatgaa aaccctggcg ttttaattcg
     5221 agcgcgcttt ccaggccaat tccactggaa catccggtaa ttaagaccga tttttgcata
     5281 actttacctg tcaggatctc cgttgcttta tgagtcatga tttactaaag gctgcaactg
     5341 cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg ttgggatgaa taccgtcatc
     5401 ctgcatccat tgtggcttga ggtagacctc ttccataaaa aagggcagca gcggaacatc
     5461 aaactctttg gcgagtttgg ggtaaatggc gctaaaggct tcattataac ggcgaccata
     5521 gtttgcaggc agacgtattt gcattaacaa tggttcagcg ttggcggctt tgacatcctg
     5581 caaaatctgg cgcagcgttt gctcggtttg ctgtggctga aaaccacgca aaccgtcatt
     5641 gccgcccagt tcaaccagca cccagcgcgg ctgatgctgt ttcagcagag ccggaaggcg
     5701 cgccagccct tgttgcgagg tgtcgccgct gatgctggca ttaactaccg acgttttact
     5761 ctgccactta tcattcaaca aggcaggcca ggccgcgctg gcagacattc gatacccggc
     5821 gctcaggcta tcacccagaa tcaataacgt gtccgctgcg gcggcacgga aggttaacag
     5881 gaccaggaac aggaagggca aatgccagcg gaaaacattg ttgaagttca tcatcttaag
     5941 aagtccgtcg gtcaggggga gcatgaactc tccatcctca ccggagttga gctggttgtc
     6001 aaacgtggcg agaccatcgc gctggtgggc gagtcgggat cgggtaagtc aaccttgctg
     6061 gcgatcctcg ccgggctgga tgacggcagc agtggcgaag tgagtctggt gggacaaccg
     6121 ctacataata tggacgaaga agcgcgggca aagttgcgcg cgaagcacgt cggctttgtt
     6181 tttcagtcat ttatgctaat tcctaccctt aacgcgctgg aaaacgtcga gcttccggcg
     6241 ctgctgcgcg gtgagagtag cgcggaaagt cgtaacggag cgaaagcgtt gctcgaacag
     6301 ttagggctgg gtaaacgtct ggatcatctt ccggcacagc tttccggcgg tgaacagcaa
     6361 cgagtggcgc tggcacgagc ctttaacggt cgacctgatg tgctgtttgc cgacgaaccc
     6421 accggcaacc ttgaccgcca gaccggcgat aaaattgccg acctgctgtt ttccctcaac
     6481 cgtgagcatg gcaccacgtt gattatggtg acccacgacc tgcaactggc ggcacgctgc
     6541 gaccgctgct tacggctggt gaacgggcag ttgcaggagg aagcatgatt gcacgctggt
     6601 tctggcgcga atggcgttcg ccgtcgctat taattgtctg gctggcgcta agcctggcgg
     6661 tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat ggagaagggc ttaagccaac
     6721 aaagccggga gtttatggcg ggcgatcggg cgttgcgcag ttcgcgcgaa gtgccgcaag
     6781 catggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg caagcagctg actttcgcca
     6841 ccatgacatt tgcaggcgac acaccgcagc tggcgaacgt caaagcggtg gatgatatct
     6901 acccgatgta tggcgatctg caaaccaatc cccctggcct gaaaccgcag gcgggcagcg
     6961 tattgctggc cccacgcctg atggcactgc ttaacctgaa aacgggcgac accattgacg
     7021 tgggcgatgc caccttgcgg attgccggag aagtgattca ggaaccggat tccggtttta
     7081 accccttcca gatggctccg cgtctaatga tgaatctggc tgatgtcgat aaaaccggag
     7141 ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt cggcggcagc gagaaccagc
     7201 tcgacagcta tgagaaatgg ttgttacctc agcttaaacc cgaacaacgc tggtacggtc
     7261 tggaacagga cgaaggcgcg ctggggcgat cgatggaacg ctcgcaacag ttcctgctgc
     7321 tttcggcgct tctgaccttg ctgctggcag tggcagcagt ggcggtagcg atgaatcatt
     7381 actgtcgcag tcgctacgat ctggtggcga tcctcaaaac gctgggggca gggcgagcgc
     7441 aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct ggcgctttca gccgttaccg
     7501 gtggggcgat aggcctgttg ttcgaaaacg tgttgatggt gctgctcaag cccgttctgc
     7561 ctgctgcact accgcctgcc agtctctggc cgtggctgtg ggcgcttggc accatgacag
     7621 tcatctcgct gctggtgggg ctacgaccgt accgcttgtt gctggcaacg cagcctttac
     7681 gcgtattacg taatgatgtg gtagcgaacg tctggccgct gaagttttat ctgccgattg
     7741 tcagtgtggt ggttgtgctg ctgcttgccg gattaatggg cggcagtatg ctgctctggg
     7801 cggtgctggc gggcgcggta gtactggctt tgctgtgcgg tgtgctgggc tggatgctgc
     7861 tgaatgtact tcgccgcatg acgctgaaat cgctgcctct gcgcctggcg gttagccgcc
     7921 tgttacgtca gccgtggtca acgttaagcc agctttcagc tttttcgctc tcctttatgc
     7981 tgctggcgct gctgctggtg ttgcgtggcg atctgctaga ccgctggcaa caacagctac
     8041 cgccagaaag cccgaactac tttttaatca acatcgccac agaacaggtt acgccgctaa
     8101 aagcgttcct cgcggaacat cagatagtct cggaatcgtt ttatccggtg gtgcgagcgc
     8161 ggctgacggc gattaacggc aagccgacag aaggcaatga agatgaggcg ctcaaccgcg
     8221 aactcaatct tacctggcaa aatacgcggc ccgatcataa tccgattgtc gccggtaact
     8281 ggccgccaaa agctgatgaa gtgtcgatgg aagaggggct ggcgaaacgc ttaaacgtta
     8341 tcctcggcga taccgtgact tttatgggcg atacccagga gttccgcgct aaagtgacca
     8401 gcctgcgcaa agtggactgg gaaagtttgc ggcctaattt ctattttatt ttcccggaag
     8461 gggcattaga cgggcaaccg cagagctggc ttaccagttt ccgctgggag aatggcaacg
     8521 gcatgttgat gcaactcaac cgccagttcc cgaccattag cctgttagac attggcgcga
     8581 ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag gtgatggtag
     8641 tgctggtcac cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg ggaatgcgtc
     8701 agcgtcatca ggagctggtg gtgtggcgca cactcggtgc ggggaaaaaa ctactgcgta
     8761 ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc cggcctggtg gccgcaattg
     8821 gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt tgattttccg tgggagccag
     8881 actggcgatt gtggattgtt ctgccgtgca gcggtgcgct gctgctgtcg cttttcggcg
     8941 gctggctggg tgcgcgactg gttaagggta aggcgctgtt caggcagttt gcggggtgat
     9001 gacaggtgtt agattgcctg atgcgctacg cttatcaggc ctacgaagag cgtgcaatat
     9061 attgattttg caagattgtg taggccggat aaggcgttta cgccgcatct ggcatgaaca
     9121 gtgtgcattt tggcaataaa tctgaaacca actgtcatgt cggtttttta aaggccgaag
     9181 gtggtaaacc ttcggcctta gcgacgtttc ctcagttatt caataaactg acggatcagt
     9241 tcctgcagcg cgattagcgc cttaagaacg atgagaactt tatcgagaaa agttttcatc
     9301 cgttgctccc ttaagggaga aagacaatca cagcttaccc ttacgttgcc tgtaactatg
     9361 attttcttca tgtgctagat gaggactgcc tctctccaaa ttagagcacc agaatcagca
     9421 ccacctgtat cctgaccagg acttaaaagt acttttccgc actgcggtaa tcccttgagg
     9481 cagtatcact actagcgtcg ggattgtatc gccaacatag ctgtttgaat atgtaggcat
     9541 cctgacttat aattcacttg ctagatgagg actgtttcat ttctgcccgg aaccaccaag
     9601 tcttgggcgg aaaaaataaa aaaaccgact gtcatgtcgg tttttttata tctgtattct
     9661 ggtgcaacca ttctgttctc acgacaatcg ccgtttcaga ataccgccca tcactgcgcc
     9721 ttaacccatt ccgccacttc cgcccactca ccgcgaaaga caactttttc cgcttttttc
     9781 tcaagctgat agcgatacat tgggtcgtaa tactcttcaa gtaacggcac cagccaggcc
     9841 agatgaccgt cggtgctgcc ggtggtgagt tgcgttgtca gtgctgcatc cagccttgca
     9901 gccagttcgt tatagcgctg tagccccagc cgacgcttaa tagccgaaag tccgtgatgc
     9961 aggtattcgc aatactcctg ccagccctgt tcgtcgccgt acgcgtgggt aaaatcatga
    10021 tgcatacgca agaaatactc ttcgttcagg cgctcaagac ggatctcaaa cggatcttct
    10081 accaccgcaa tcgccgcctg agtcattcgc tcgcgcaggc attccggcag gtgattcgaa
    10141 ccgatcatcc ggctttcgtc ttccagcacc cacaggcgca aattctgacg ggcgtcggtt
    10201 tttagcattt cggcagccag caggttttca aaactcgcct ggctaagttg tggttgtaag
    10261 gtgcgaccaa acgccgaacc gcgatgacgc gccaaccctt ccagatcaac accgttcggc
    10321 tgttgctgca ctaacagcgt tttaccgctg ccggtacaac cgccaatcag cactatcggt
    10381 ttttgtgcca gttcaatagt cgcctgaatc gcggtctggc gcagtgcctt ataaccgcct
    10441 tccaccagcg gataatcaat ccccgctgca tgcaaccagc tttgcacaat atgtgagcgc
    10501 tgaccgccac gggcgcagca gagaatacct tgcggatttt gcaggcacgc tgcccgccag
    10561 gcgtctatac gctgctgacg aatttcaccc gccaccagtt tatgccccag cgccagcgcc
    10621 gcgtctgacc cttgctgttt atagcaggtg ccaacggcgg cgcgttcatc gttattcatt
    10681 aacggcagat tgatagcggc gggcattgcg ccttgctcaa actcgacagg ggcgcgaaca
    10741 tcaataatgg gcgtatcagc aatcagcagg gcacgatagt cctgttccgt gtgtctctct
    10801 tgcatagtta aaagtgaacc tcaaatcagc ttgcgcgcta ttttacgcgc caacgcgcaa
    10861 ggaaacttga tttttaactg cgtgggttgc cgaaaatttc taaaaatccg ctgatttccg
    10921 gcctgcgctg ggtaaacaag gtcacaatat cttctaccgc tttgccgctg ccaaatttgc
    10981 gccatgccag actcaatggc gaaggagggc gcatcgttgg gattacccgg ctgaccagtt
    11041 gttgattatc gatcattgac tggcaaagcg attttggcaa aaaaccaatg ccaacgcccg
    11101 ccagatgggc ggcgattttc gtttccatat caggaacaat aatctctttt tgacccggca
    11161 atcgccaggc gacgcgtttg gttaaggtgc gggcgctgtc ttcaatattg accgccggaa
    11221 agcgccgcaa ctgcgcttct gttagcggct cttcaacgtt cgctagcgga tgatccgccg
    11281 ccatgacaaa gcgccattgc accgatccta agggatcaag actaaaggta tttgccagcg
    11341 cctcagttcc cgtgacgccg atagccagcg aaaaaccttc gtacaatagc gagtcccaga
    11401 cgcccatata gatttgtcgg gagatgtgaa actgggtaaa ggggtaacgc tcattcagcc
    11461 acgccagcaa ctgggcgacg gcctgggggt tatagagcag gttgttgatg acaatattca
    11521 cctggcgttc cacgccatca ttcacctgtt gcaactcgct gggcatactt tccagccagc
    11581 tcagccagtc tctggcctgg gaaagtagat gctcgccagc cgctgtcaac gtcacgctgc
    11641 gagtcgtgcg gaaaaacagc gctactccgg tattctcttc cagaagttta atgcgataac
    11701 tgatcgtcgc cgtggtttta cataatcgtt ctgccgcttt tgaaaaactt cctgtttcag
    11761 caaccgcaat gaaagtccgc aaggtttctg gatcgaacat cttcaggtat ccccttttaa
    11821 atccgcaagt tgcgtgattt tcttatcctc tgatttatca gtatttttac atgataaccc
    11881 tgttcaattt gtggactaaa tctagttttg gaaaaatatt ccaactttgt gttgatgttg
    11941 ttctcttaag gttttagatt gcctgttatt gaaaccaagc tgaccggtcg gcggtggttg
    12001 aacggaatta tgttacaagg acaaaaagat gaaacttcag gtattaccgt taagtcagga
    12061 agcctttagt gcttatggcg acgtaatcga aacgcagcaa cgggattttt tccatattaa
    12121 caatggcctg gtggagcgtt accacgattt ggcgctggtt gagattcttg agcaagaccg
    12181 tacgcttatc agcattaacc gcgcgcaacc ggcgaatctg ccgctgacca ttcacgaact
    12241 cgaacgtcat ccgctgggta ctcaggcctt tatcccgatg aaaggtgagg tgtttgtggt
    12301 ggtcgtggcg ttaggtgacg acaaaccaga cctgtcaacc ctgcgggcgt ttattaccaa
    12361 cggcgaacag ggagtgaatt accatcgtaa cgtctggcat cacccacttt tcgcctggca
    12421 gcgcgtcacc gattttctga ccatcgatcg cggcggcagt gacaactgtg atgttgaaag
    12481 tattcctgaa caggaactct gttttgcgtg acgcctgcaa ccgacttgca taagataaac
    12541 taattgttca ttgtttatgc tcacttgtag gtcggagtta acgtaggtat gacggaagtt
    12601 agacggcgcg gcaggccagg acaggcggag cctgtggcac agaagggcgc acaggcatta
    12661 gagcggggaa ttgcgattct gcaatatctg gaaaaaagtg ggggaagttc atcggttagc
    12721 gatatttctc tcaatctgga tttgccgctc tccacgacct ttcgcttgct gaaggtttta
    12781 caggcagcgg attttgtcta tcaggacagt cagttaggct ggtggcatat aggattaggt
    12841 gtttttaacg tcggtgcagc gtacatccat aaccgcgatg tcctctccgt cgccgggccg
    12901 tttatgcgcc gtctgatgtt actttccggc gaaacggtca atgtcgcgat ccgtaacggc
    12961 aatgaagcgg tattaattgg tcagttagag tgtaaatcga tggtcaggat gtgtgcgcca
    13021 ctgggcagtc gtctgccact gcatgcttcc ggtgcgggca aagcgctgct ttatccgctg
    13081 gcggaagagg agttgatgag catcattctg caaaccggtt tgcagcagtt tacgccaact
    13141 acgcttgtgg atatgcccac cttactgaag gacctggagc aagcgcgtga actgggctat
    13201 accgtagata aagaagagca tgttgtaggt ctgaattgca tagcttcagc aatttacgat
    13261 gatgtaggta gtgttgttgc cgctatctcc atctccgggc cttcatcaag actgacagaa
    13321 gatcgttttg tcagtcaggg tgagctggtc agagacaccg cccgcgatat cagcacggcg
    13381 ttgggactga aagcacatcc atagtgtctg tcgcatcccg ctctgcggag cgggtttttt
    13441 tgacaaaatt tgaaagttgg aaaatttttc caattaatag aggtaggaat aaaatggcaa
    13501 aaatgagagc cgttgacgcg gcaatgtatg tgctggcgaa agaaggtatc accaccgcct
    13561 tcggtgttcc gggagctgca atcaatccgt tctactcagc gatgcgtaag cacggcggta
    13621 ttcgtcacat tctggcgcgt catgtggaag gtgcttcgca catggcggaa ggttataccc
    13681 gcgcaacggc agggaatatc ggcgtatgtc tagggacttc cggtcctgcg ggcacggaca
    13741 tgatcaccgc gctctattct gcttctgctg attccattcc tattctgtgc attaccggcc
    13801 aggctccgcg cgcccgtctg cataaagaag attttcaggc cgtagatatt gaagcaattg
    13861 ctaaaccggt cagcaaaatg gcggttacag tacgtgaagc ggcgctggtg cctcgcgtgc
    13921 tgcaacaggc atttcacctg atgcgttctg gtcgtccggg tccggtactg gtggatttac
    13981 cgttcgacgt tcaggttacg gaaatcgagt ttgatcctga catgtacgaa ccgctgccgg
    14041 tctacaaacc tgctgccagc cgtatgcaga tcgaaaaagc tgtagaaatg ttaatccagg
    14101 ccgaacgtcc ggtgattgtt gccgggggcg gggtaatcaa tgctgacgca gctgcactgt
    14161 tacaacagtt tgctgtactg accagcgttc cggtgatccc aacactgatg ggctggggct
    14221 gtatcccgga cgatcatgaa ctgatggccg ggatggtcgg tctgcaaacc gcgcatcgtt
    14281 acggtaacgc aacgctgctg gcgtccgaca tggtgtttgg tatcggtaac cgttttgcta
    14341 accgtcatac cggttcggta gagaaataca ccgaagggcg caaaatcgtt catatcgata
    14401 ttgagccgac gcaaattggc cgcgtgctgt gtccggatct cggcattgtc tctgatgcta
    14461 aagcggcgct gacactgctg gttgaagtgg cgcaggagat gcaaaaagcg ggtcgtctgc
    14521 cgtgtcgtaa agaatgggtc gccgactgcc agcagcgcaa acgcactttg ctgcgcaaaa
    14581 cccacttcga caacgtgccg gtgaaaccgc agcgcgtgta tgaagagatg aacaaagcct
    14641 ttggtcgcga tgtttgttat gttaccacca tcggtctgtc acaaatcgct gcggcacaaa
    14701 tgctgcatgt ctttaaagac cgccactgga tcaactgtgg tcaggctggt ccgttaggct
    14761 ggacgattcc ggcggcgcta ggggtttgtg ccgctgatcc gaaacgcaat gtggtggcga
    14821 tttccggtga cttcgacttc cagttcctga ttgaagagtt agccgttggc gcgcagttca
    14881 acattccgta catccatgtg ctggtgaata acgcctatct cggcctgatt cgtcagtcac
    14941 aacgcgcctt tgacatggac tactgcgtgc aactcgcttt cgagaatatc aactccagcg
    15001 aagtgaacgg ttacggcgtc gaccacgtaa aagtagcgga aggtttaggt tgtaaagcga
    15061 ttcgcgtctt caaaccggaa gatattgcac cggcctttga acaggcgaaa gccttaatgg
    15121 cgcaatatcg ggtaccggta gtcgtggaag ttattctcga gcgtgtgacc aatatttcga
    15181 tgggcagcga actggataac gtcatggaat ttgaagatat cgccgataac gcagcggatg
    15241 caccgactga aacctgcttc atgcactatg aataagggag ataaataatg ttacgtttct
    15301 ctgctaattt atcgatgtta tttggtgaat atgattttct cgcccgtttt gagaaagctg
    15361 cgcagtgtgg ttttcgcggc gttgaattta tgtttcctta tgactacgac attgaagaat
    15421 taaaacaggt gctggcgagt aataaactcg aacatacgct gcacaattta ccggcgggtg
    15481 actgggcggc gggggagcgc ggtattgcct gtattcctgg tcgtgaagaa gagtttcggg
    15541 atggcgtagc agcagcgatt cgttatgccc gtgcgctggg taataaaaaa attaactgtc
    15601 tggtcggtaa aacgccggct ggtttcagca gtgaacagat tcacgcaacg cttgtagaaa
    15661 acctgcgtta tgccgcgaat atgctgatga aagaagatat tttattactg attgaaccta
    15721 ttaaccattt tgatattcct ggtttccatc tcaccggaac tcggcaggcg ctgaaattga
    15781 ttgatgatgt tggttgctgc aatttgaaaa ttcagtatga catttatcac atgcagcgga
    15841 tggaaggtga attaaccaac accatgactc agtgggccga taaaattggt cacctgcaaa
    15901 ttgccgataa tccgcatcgc ggcgaaccgg ggaccggaga aattaattat gattatctct
    15961 ttaaggtaat cgaaaattct gactacaacg gttgggttgg ttgtgaatat aaaccccaaa
    16021 ccaccacgga agccggttta cgctggatgg atccgtaccg ttaaaacgta acgctattca
    16081 ggcaatgctt ttttaggccg ctaagttggc aggggatcgt gttgtctgaa tccaggaaaa
    16141 gcaaaattta aaagaggtta attatgaaac tgggatttat tggcttaggc attatgggta
    16201 caccgatggc cattaatctg gcgcgtgccg gtcatcaact gcatgtcaca accattggtc
    16261 cggttgctga tgaattactg tcactgggtg ccgtcagtgt tgaaactgcg caccaggtaa
    16321 cggaagcatc ggacatcatt tttattatgg tgccggatac accacaggtt gaagaagttc
    16381 tgttcggtga aaatggttgt accaaagcct cgctgaaggg caaaaccatt gttgatatga
    16441 gctccatttc cccgattgaa actaaacgtt tcgctcgtca ggtgaatgaa ctgggcggtg
    16501 attatctcga tgcgccagtc tccggcggcg aaatcggcgc gcgtgaaggt acgttgtcaa
    16561 ttatggttgg cggtgacgaa gcggtatttg aacgtgttaa accgctattt gaactgctcg
    16621 gtaaaaatat cacccttgtg ggcggtaacg gcgacggtca aacctgcaaa gtggcaaatc
    16681 agattattgt ggcgctcaat attgaagcgg tctctgaagc tctgctgttt gcttcaaaag
    16741 ccggtgcaga cccggtacgt gtgcgccagg cgctgatggg cggctttgct tcctcacgta
    16801 ttctggaagt tcacggtgag cgcatgatta aacgcacctt taatccaggt ttcaaaattg
    16861 cgctgcacca gaaagatctc aacctggcac tgcaaagtgc gaagacactg gcgctgaacc
    16921 tgccaaacac cgcgacctgc caggagttat ttaatacctg tgcggcaaac ggtggcagcc
    16981 agttggatca ctctgcgtta gtgcaggcgc tggaattaat ggcgaatcat aaactggcct
    17041 gatacccgca ataaaaatgg ccgatatcag aatatgaatc ggccagcaat attaaaaaag
    17101 aaagcagcca aagatgttgc ttcagtatta aaaataatat ttttatttta tttgttcctc
    17161 atagctagat taaaacaacg ttattcgata cgtgaaatta agagggattt atggaacatc
    17221 agagaaaact attccagcaa cgcggctata gcgaagatct attgccgaaa acgcaaagcc
    17281 agcggacctg gaaaacattt aactatttta ccttatggat gggttcggtt cataacgttc
    17341 ccaattatgt gatggtcggc ggctttttta ttctcggctt gtctaccttt agtattatgt
    17401 tggcaattat cctcagcgcc tttttcattg ccgcggtaat ggtattaaac ggtgctgcgg
    17461 gcagtaaata cggcgtaccg tttgccatga tcctgcgtgc ttcttacggc gtacgtggcg
    17521 cactgtttcc cggattatta agaggcggta ttgctgccat tatgtggttt ggcctgcaat
    17581 gttacgcggg gtcactggcc tgcttgattc tgattggcaa aatctggccg ggatttttga
    17641 ctctcggtgg tgatttcact ctgttaggcc tttctctacc gggcttaatt actttcttac
    17701 tcttctggct ggtcaacgtt ggtataggtt ttggcggtgg caaagtttta aataaattca
    17761 ctgccattct taacccgtgc atctatatcg ttttcggcgg tatggcgatt tgggcgattt
    17821 cgctggtcgg gctcggtcca atctttgact acattccgag cggtattcag aaagcagaaa
    17881 acagtggatt cttgttcctg gtggtgatta acgcggtagt tgcggtctgg gcggcaccgg
    17941 cggtgagcgc atccgacttt acgcaaaacg cccactcgtt tcgtgagcaa gcgctggggc
    18001 aaacgctggg tttagttgtg gcctatattc tgtttgcggt cgctggggta tgtattattg
    18061 ccggagccag tattcactac ggcgctgaca cctggaacgt gctggatatt gttcagcgtt
    18121 gggacagcct gtttgcctcg ttctttgcgg tactggttat tctgatgacg accatttcca
    18181 ctaacgccac cggtaatatt attccggccg gttatcagat tgctgccatt gcaccgacaa
    18241 aactgaccta taaaaacggc gtactgattg ccagtattat cagcctgctg atctgcccgt
    18301 ggaaattaat ggaaaatcag gacagtattt atctgttcct cgatatcatc ggcggaatgc
    18361 tgggtccggt aattggtgtc atgatggcgc attattttgt ggtgatgcgt gggcaaatta
    18421 atcttgatga actgtacacc gcacctggcg attataaata ttacgataac ggttttaacc
    18481 tcaccgcgtt ttcagtaact ctggtggccg ttattttatc tcttggcggt aagtttattc
    18541 cctttatgga accgttatcg cgtgtttcat ggtttgtcgg cgtcatcgtc gcctttgcgg
    18601 cctacgcctt attaaagaaa cgtacaacag cagaaaaaac aggagagcaa aaaaccatag
    18661 gttaattaat cccgatattg aacattgagt taaaaaccaa tctgtatttt acaaggagtt
    18721 tgttatgtct tttgatttaa ccattaaaaa cggcaccgtt attttagaaa acgaagctcg
    18781 cgttgtagat atcgccgtta aaggcggcaa aattgctgct atcggtcagg atctgggcga
    18841 tgcaaaagaa gttatggatg cgtctggtct ggtggtttcg ccgggcatgg ttgatgcgca
    18901 cacccatatt tctgaaccgg gtcgtagcca ctgggaaggt tatgaaaccg gtactcgcgc
    18961 agcggcaaaa ggtggtatca ccaccatgat cgaaatgccg ctcaaccagc tgcctgcaac
    19021 ggttgaccgc gcttcaattg aactgaagtt cgatgccgct aaaggcaagc tgactatcga
    19081 tgcggcacaa ctcggtggtc tggtgtctta caacatcgac cgtctgcatg agctggatga
    19141 agtgggcgtt gtcggcttca aatgcttcgt tgcgacctgt ggcgatcgcg gtatcgacaa
    19201 cgacttccgt gatgtaaacg actggcagtt cttcaaaggt gcgcagaagc tgggcgaact
    19261 ggggcagccg gtgctggtgc actgcgaaaa cgcgctgatc tgtgacgcac tgggcgaaga
    19321 agcgaaacgt gaaggtcgcg taactgccca tgactatgtg gcttcgcgtc cggtatttac
    19381 cgaagtggaa gcgattcgcc gcgtgctgta tctggcgaaa gttgccggtt gccgtctgca
    19441 cgtttgccat atcagcagcc cggaaggtgt tgaggaagtg actcgtgcac gtcaggaagg
    19501 tcaggacgtt acttgtgaat cctgcccgca ttactttgta ctggataccg atcagttcga
    19561 agaaattggt actctggcga agtgttcacc gccgatccgc gatctggaaa accagaaagg
    19621 tatgtgggaa aaactgttta acggtgaaat cgactgcctg gtttccgacc actcaccatg
    19681 ccctccggaa atgaaagccg gcaacatcat ggaagcatgg ggtggtattg ccggtctgca
    19741 aaactgtatg gacgtgatgt tcgatgaagc ggtacagaaa cgcggaatgt ctctgccaat
    19801 gttcggcaaa ttaatggcga ctaacgcagc agatattttc ggtctgcagc aaaaaggccg
    19861 tatcgcccca ggaaaagatg ccgacttcgt cttcattcag ccgaatagca gctatgttct
    19921 taccaatgac gatctggaat atcgccacaa agtcagcccg tatgttggcc gtaccattgg
    19981 cgcgcgtatc acgaaaacca tcttacgtgg tgatgtgatt tacgacattg aacagggctt
    20041 ccctgttgcg ccgaaaggtc aatttatcct taaacatcag cagtaatctg gcccctgcaa
    20101 tgcccgtcct tgcggcgggc attctccggt taaggtgtgt ttatgttcaa ttttgcagtt
    20161 agccgcgaaa gcctgttatc aggatttcag tggtttttct ttattttttg caacacggtt
    20221 gtggttcctc ctacactact ttctgctttt cagttgccgc aaagtagcct gcttacgctc
    20281 acgcaatatg cttttcttgc taccgcactg gcctgcttcg ctcaggcgtt ttgcggtcat
    20341 cgtcgcgcta ttatggaagg gccggggggc ctgtggtggg gaaccatcct aactatcacc
    20401 cttggcgaag catcgcgcgg gacaccgatc aacgatatcg ctaccagtct ggcggtgggg
    20461 attgcactct ccggcgtgct gacgatgttg attggtttta gcggattagg ccatcgcctg
    20521 gcacggttat ttacgccgtc ggtgatggtc ttgtttatgt tgatgctggg cgcgcagctg
    20581 accactatct ttttcaaagg tatgctcggg ctgccgtttg gcatagccga cccgaatttt
    20641 aaaattcagt taccgccgtt cgcgctctcg gtggcggtga tgtgcctggt actggcgatg
    20701 attattttcc tgccgcaacg ttttgcccgt tatggcctgc tggtcggcac cataaccggc
    20761 tggttgttgt ggtacttttg ctttccttct tcgcattcgc tctccggtga gttgcactgg
    20821 cagtggttcc cgctcggcag tggcggtgct ttgtctccgg ggattattct gacggcggtg
    20881 attacaggtc tggtgaatat cagcaatacc tacggtgcga ttcggggcac ggatgttttt
    20941 tatccgcagc agggcgcggg gaatacgcgt tatcgtcgta gctttgtggc gaccggattt
    21001 atgacactga taaccgtacc gctggcggta attccatttt caccgtttgt ttcatccatt
    21061 ggtttattaa cccagactgg cgattacacg cggcgttcgt ttatttatgg cagcgttatt
    21121 tgcctgctgg tggcgctggt tccggcactc acgcgactgt tttgcagtat ccctttaccc
    21181 gtgagtagtg cggtcatgct ggtttcttat ctgcctttac tcttttccgc gctggtgttt
    21241 agccagcaaa taacgtttac cgctcgcaat atttatcgac tcgcattgcc gttatttgtc
    21301 ggcatatttt taatggcatt accgcctgtg tatctgcaag accttccatt aacgcttcgt
    21361 cctctgctca gtaacggctt attggtcggg attttactgg ctgttcttat ggataacctt
    21421 attccgtggg aacgcatcga ataatttgtt gaaaaaggat tgataatgaa gattgtcatt
    21481 gcgccagact cttttaaaga gagcttaagt gcagaaaaat gttgtcaggc aattaaagcc
    21541 gggttttcga ccctctttcc cgatgcgcac tatatctgtt taccgatagc ggatggcggc
    21601 gaagggacag tggatgcgat ggtcgccgcg acgggcggca acatcgtgac gcttgaagtc
    21661 tgcgggccga tgggcgaaaa agtgaatgct ttttatggcc ttaccggcga cgggaaaacg
    21721 gcggtgattg agatggcggc agcaagtggc ctgatgctgg tcgcgcctga aaagcgtaat
    21781 ccgttgctgg cctccagttt tggtacgggg gagttaattc gtcatgcgct ggataacggc
    21841 attcgccata ttattctcgg cattggcggc attggcggca gtgcgacggt cgacggcggt
    21901 atgggcatgg cgcaggcgct cggtgtgcgt ttccttgatg ccgacggtca ggtgctggtg
    21961 gctaacggcg gcaatttagc gcgcgtggaa agtattgaga tggatgaatg cgatccgcgt
    22021 ctggcgaact gccatattga agtagcatgt gacgttgata acccgctggt aggggcacgc
    22081 ggcgcggcgg cggtgtttgg cccgcaaaaa ggggcaacgc cggaaatggt cgaagagctg
    22141 gaacaggggc tgcaaaatta cgcccgtgtt ttacaacagc aaactgaaat caacgtctgc
    22201 cagatggcgg gcggcggcgc tgcgggcggt atgggtattg cggcggcggt atttctcaat
    22261 gcggatatta aaccgggtat tgagattgtg ttgaatgcgg tcaatcttgc gcaggcagtg
    22321 cagggcgcag cactggtgat taccggggaa gggcgcatcg actcgcaaac ggcaggcggt
    22381 aaagcgccgt tgggtgtggc gtcggtggcg aaacagttta atgtaccggt gattggaatt
    22441 gctggcgtat tgggtgatgg cgtggaagtg gtacaccagt acggcattga cgcggtattc
    22501 agcattttgc cacgtctggc acctttagcc gaagtgctcg ccagcggtga aaccaatctc
    22561 ttcaacagcg cgcgaaatat tgcctgcgcc attaaaatag gtcagggaat taaaaactga
    22621 ctcttacctt taaagcggat gcgatttatt tcgcatcttt ttttacctga atttactaca
    22681 aaaaaagcca cggtataaac cgtggcaaaa tccaacatag ctaaaaataa tcaggcgagt
    22741 ggtatgactt aaatctctac gtcgcggtta caatctttcg agtaaatata gctgaacgct
    22801 tcaccacgcc ctacgccata accagcctgt aaagaataag cgcccataaa gatgtaatcg
    22861 ccttttttca ccgggatcca gttattgtcg aggttataaa ccccctgacc ggaaagaata
    22921 taggcaccgt gttcctgaac gtgtgtttcg atataaccat ggctggcacc tggtgcaaaa
    22981 gagaggatat gcatgttcat atcaaaacct aactctttgg gcagaaaatc cagcaggatc
    23041 acatcatcca taccttcata gtgaatgcgt tccagttcgc tggcattgcc agaaaccagc
    23101 cacggtgcat ggccttctac cggaacatag cggcgcttat ataaaaagat ttggctgtct
    23161 tcggcctggg cgttaacaaa cgtcattaag gagccaggcg ggcaataaag atagccacct
    23221 tcacttaagg caaatgtttt gccttcggct ttggcagtga tatttccaga gatcacatac
    23281 aggaacgttt caatgccttc gccaccgaag ccctgttggt tgccaccgtt ttgatgcagt
    23341 gtgaccagat aatcaacaaa agaggcaccc agctttggcg tggagaggat tgtcgcgtca
    23401 caattttcaa agcccggaat aatatttttt accagaccgt ctggtgttaa cagtgcgaaa
    23461 ttaccgtgtt tcacaatcgc acggttagcc agtaaatcat cgcggtaacc ggtgacgtta
    23521 tttaaatatc ccatttatga ctccttattt ctgccaggca agttgataaa gcatgagtgc
    23581 caacgttttg accccttcgg caaggtcggt aatattggtg cgttctgccg ggttatggct
    23641 gatcccgttg atgctgggaa tgaaaatcat gcaggtcggc acgcgaggcg cgaaaatttg
    23701 tgcgtcgtgc ccggcaccac tgtgcatcac ccggtaattc agtttttcgc tttcacacaa
    23761 ttctgtcagg gtggcgacca ggtccttatt catcggcacc ggttcttcgt ccatccataa
    23821 atcgatatca ataccaatgt ccatttcatc gcaaatcgcc cgcatgtcgt tttctaactg
    23881 ttgggtgaaa tcacgcagca cggcggcgtc ggtatgacga caatcaatgg tgaacgtggt
    23941 tttacccggc accacattca ccgtattcgg gcgcggctct actttcccaa aggtcagcac
    24001 cagcgggtcg cccatctttt tcgctttttc gatcgcctga tggcaaatgc gactgaaagc
    24061 gtaaaccgta tcacgacgat aacccatcgg cgtggtgcct gcatggtttg attcgccgtt
    24121 cagcgtcacc gtatagcgac gttgcccgac aattgcattc accacgccaa ttgattgccc
    24181 attactttcc agcacacagc cctgttcgat atgcagttcg acaaaggctt taatatcctg
    24241 acgcggagtt agcggggcgt ccggcagagt aaatccgcaa gccttcatcg catcgacaaa
    24301 actatttcct ttggcatcac agatattccg cacgtcgtca ggattcgcca gtccaaagat
    24361 atttttactg ccccagaaaa catacgggaa gcggctgcct tcttcttccg ccatcgccac
    24421 cacttcaacc gtacgcagcg gtgcgccgta ttgagttttc agcctgtcaa ttgccagcca
    24481 cgccgccagc ccgccgaatt gcccgtcgag gttaccgccg ttaaccacgg tatcgatatg
    24541 cgaaccgctc agaaccactt cctgtggata ttcggtgcca ctcaggcgac cgtataaatt
    24601 ccccacttca tcgaaacgtg tttccagccc acttgcggcc attctttttt taaattgctg
    24661 ctgggtttcc agccattccg gcgaataaag taaacgggtc atcccacccg ttgggtcagc
    24721 gccaaatgag gaaagccagg gcagcgcttc ttctatggct tgacgaaaat gtgtaatcat
    24781 aagaaagtcc tgtctcaata attattgcgc aaagggattt ttcgtttcgt atgacgtgtt
    24841 ataaagcgcg tcggaaatta aatactggta aatatcatca acaatttcga tgccttcgac
    24901 ggcggctttg cgttgtttaa tatcctgatc ctgtccggga taataaacct gatttaaacc
    24961 gggcgcgggg gtaatggcat ttaattcgcg catggtctgg ctaagatgtt gacggaataa
    25021 ttcgctggag gagaaaaagt tcgggttaat aactacatgt aattgcccca aattacgccc
    25081 ggcgtgtaaa tcgtcataca tcgaactaac ctgtcgcccg aacggtaagc cgagtaagac
    25141 gcctgagagg acgtcaatca tcatcaggcc ataccctttc ggcccggctg cggggagcag
    25201 agcatgtacc gcgaacggat cggttgttgg tgcaccgttt ttatcgaccg cccaggtatc
    25261 cgggatagac atattacgcg agcgggcgtc gagcactttt ccccatgcct gtacggtagt
    25321 cgccatatca aaggtaagga tctcgtcgcc ttctcccggc gcggcaaagg ccagtgggtt
    25381 agtaccgtag taaatttccg cgccgccaaa cggcaccacc atcggatcgg actggcacat
    25441 cgaaatgcca attaatccgg cgcgggctgc ctgctgcaca aaataagaga ttgcgccgct
    25501 gtgacccatc cggctgatac cgaccaccgc aacgccattt tgctgggcgg ttttgatggc
    25561 atgttccata cccattttcg ccgcgacctg tccggcggca ttgtcggcat gtaaaattgc
    25621 cgagcacggc ccggtttcct caagacgaaa ctccggttcg cggttggtgc cgccttttga
    25681 aatgcgttcc gcgtagtatt ccacgcgcac cgcgtcatga gagtggatcc ctctggcatc
    25741 ggcgtaaacc aatacttcag ccacggttgc agcgtgctca cgttttaacc cagcctggca
    25801 gagtttattc tcaattagct ggtggagtgt ttcccgactg attttcatct gtcttccttt
    25861 ttaacgacgg tgtgaagcat gactgcaatt aacatacagg gaaaatatct ggattatgtg
    25921 atccagacag gcaaaaaaat atagttagaa tttatttgat aatcttcgcg cttttaacct
    25981 ggttgtttaa a
//
LOCUS       DAZUGK010000047        25194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZUGK010000047 DAZUGK010000000
VERSION     DAZUGK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.047"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..1453)
                     /gene="rhlE"
                     /locus_tag="ACHY5W_001729"
     CDS             complement(89..1453)
                     /gene="rhlE"
                     /locus_tag="ACHY5W_001729"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFS4446640.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSSSAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1682..2353
                     /gene="cecR"
                     /locus_tag="ACHY5W_001730"
     CDS             1682..2353
                     /gene="cecR"
                     /locus_tag="ACHY5W_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFS4446641.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2356..3351
                     /gene="hlyD"
                     /locus_tag="ACHY5W_001731"
     CDS             2356..3351
                     /gene="hlyD"
                     /locus_tag="ACHY5W_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFS4446642.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3344..5080
                     /gene="ybhF"
                     /locus_tag="ACHY5W_001732"
     CDS             3344..5080
                     /gene="ybhF"
                     /locus_tag="ACHY5W_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4446643.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5073..6206
                     /gene="ybhS"
                     /locus_tag="ACHY5W_001733"
     CDS             5073..6206
                     /gene="ybhS"
                     /locus_tag="ACHY5W_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4446644.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6217..7323
                     /gene="ybhR"
                     /locus_tag="ACHY5W_001734"
     CDS             6217..7323
                     /gene="ybhR"
                     /locus_tag="ACHY5W_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4446645.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWVNPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7285..7695)
                     /gene="ybhQ"
                     /locus_tag="ACHY5W_001735"
     CDS             complement(7285..7695)
                     /gene="ybhQ"
                     /locus_tag="ACHY5W_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFS4446646.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTIVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7828..8589
                     /gene="ybhP"
                     /locus_tag="ACHY5W_001736"
     CDS             7828..8589
                     /gene="ybhP"
                     /locus_tag="ACHY5W_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4446647.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPACTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8586..9827
                     /gene="clsB"
                     /locus_tag="ACHY5W_001737"
     CDS             8586..9827
                     /gene="clsB"
                     /locus_tag="ACHY5W_001737"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFS4446648.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVRAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9827..10783
                     /gene="ybhN"
                     /locus_tag="ACHY5W_001738"
     CDS             9827..10783
                     /gene="ybhN"
                     /locus_tag="ACHY5W_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFS4446649.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVIYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10819..11532)
                     /gene="ybhM"
                     /locus_tag="ACHY5W_001739"
     CDS             complement(10819..11532)
                     /gene="ybhM"
                     /locus_tag="ACHY5W_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4446650.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLIGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHNEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11737..12441)
                     /gene="ybhL"
                     /locus_tag="ACHY5W_001740"
     CDS             complement(11737..12441)
                     /gene="ybhL"
                     /locus_tag="ACHY5W_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4446651.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFIAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDSSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12577..13029)
                     /gene="moaE"
                     /locus_tag="ACHY5W_001741"
     CDS             complement(12577..13029)
                     /gene="moaE"
                     /locus_tag="ACHY5W_001741"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFS4446652.1"
                     /translation="MAGTKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13031..13276)
                     /gene="moaD"
                     /locus_tag="ACHY5W_001742"
     CDS             complement(13031..13276)
                     /gene="moaD"
                     /locus_tag="ACHY5W_001742"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFS4446653.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13269..13754)
                     /gene="moaC"
                     /locus_tag="ACHY5W_001743"
     CDS             complement(13269..13754)
                     /gene="moaC"
                     /locus_tag="ACHY5W_001743"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFS4446654.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13757..14269)
                     /gene="moaB"
                     /locus_tag="ACHY5W_001744"
     CDS             complement(13757..14269)
                     /gene="moaB"
                     /locus_tag="ACHY5W_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFS4446655.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14291..15280)
                     /gene="moaA"
                     /locus_tag="ACHY5W_001745"
     CDS             complement(14291..15280)
                     /gene="moaA"
                     /locus_tag="ACHY5W_001745"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFS4446656.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15268..15412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15677..16585
                     /gene="yvcK"
                     /locus_tag="ACHY5W_001746"
     CDS             15677..16585
                     /gene="yvcK"
                     /locus_tag="ACHY5W_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4446657.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16777..18798)
                     /gene="uvrB"
                     /locus_tag="ACHY5W_001747"
     CDS             complement(16777..18798)
                     /gene="uvrB"
                     /locus_tag="ACHY5W_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4446658.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19377..20054)
                     /gene="bioD"
                     /locus_tag="ACHY5W_001748"
     CDS             complement(19377..20054)
                     /gene="bioD"
                     /locus_tag="ACHY5W_001748"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS4446659.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKAPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20047..20802)
                     /gene="bioC"
                     /locus_tag="ACHY5W_001749"
     CDS             complement(20047..20802)
                     /gene="bioC"
                     /locus_tag="ACHY5W_001749"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFS4446660.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20789..21943)
                     /gene="bioF"
                     /locus_tag="ACHY5W_001750"
     CDS             complement(20789..21943)
                     /gene="bioF"
                     /locus_tag="ACHY5W_001750"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFS4446661.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21940..22980)
                     /gene="bioB"
                     /locus_tag="ACHY5W_001751"
     CDS             complement(21940..22980)
                     /gene="bioB"
                     /locus_tag="ACHY5W_001751"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFS4446662.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQSAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23067..24356
                     /gene="bioA"
                     /locus_tag="ACHY5W_001752"
     CDS             23067..24356
                     /gene="bioA"
                     /locus_tag="ACHY5W_001752"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFS4446663.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNVAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24415..24891
                     /gene="ybhB"
                     /locus_tag="ACHY5W_001753"
     CDS             24415..24891
                     /gene="ybhB"
                     /locus_tag="ACHY5W_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFS4446664.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         5834 a         6754 c         6612 g         5994 t
ORIGIN      
        1 caataaaatc agacatgtca tgcatgtctg accgtgttac ctgaataatg ggcaaaagcc
       61 tgtgatactc ttttgccctt acatggaatt actgcgcagc ggcaggttta cgcggacggc
      121 ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac ggtttttctg
      181 caggttttgc gcttgaagat tttgcgccac cttctcctcg gcgtggttgc tgttgaccgc
      241 gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccattc tggatcggtt
      301 cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg atctcttttt
      361 tcagcaattt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc aacgacaacg
      421 cttctccggt ggctgcggca cgaccggtac gcccgatacg gtggacataa tcttctggta
      481 cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga ccgcgcgcag
      541 cgatgtcagt tgccaccagt acacgaatat cgcccgattt aaaatcggcc agcgcacggg
      601 tacgcgcacc ttgcgattta ttaccgtgga tcgccgcact acggatgcca tctttattaa
      661 gctgttcagc gagatggtta gcgccatgtt tggtacgggt aaacaccagc acctgctgcc
      721 agttcccttt cccaatcatg tgcgacagca attcgcgttt gcgcttctta tcaacaaagt
      781 gaacatgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg atttccagcg
      841 ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc gcggagaaca
      901 acaggttctg gcgcttcgca ggtagttttg ttaacacgcg acggatatcg tggataaagc
      961 ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga tccagcttca
     1021 ctgcattctg atgttccagg tccagcaaac gtcccggcgt tgccaccagc acatcaacgc
     1081 cgccgcgcaa cttcatcatt tgtgggttaa tgctgacacc gccaaacacc accagcgaac
     1141 gaatgttcag gtatttgctg taatcacgga cgttttcgcc aatctgcgca gccagttcac
     1201 gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc ggctggcgag
     1261 tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttgccggtg ccggtctggg
     1321 cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc tgaatagggg
     1381 tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt aaacccaaag
     1441 aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt tttcagggag
     1501 atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt gtagcagttt
     1561 ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct tagccatact
     1621 ggacaactcc catatggagt cataatctta atcaatcatt tgattaagaa tggactgtgc
     1681 gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc tgattgctgc
     1741 cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga tagccgccca
     1801 ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag atttgtacct
     1861 cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc atgccgagga
     1921 agccgaacgc ttgttcgccc aaccacagcc tgatcgggct gccatccgtg aactgatcct
     1981 tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca acctcagcaa
     2041 gtttatctcc cgtgagcagc tctctcccac ggcagcctac cacctggtgc acgaacaggt
     2101 gattagtccg ctacacagcc acctgacacg cctgattgcc gcctggaccg gctgcgacgc
     2161 caatgacacc cgcatgatcc ttcataccca tgcgttgatt ggtgagattc tggcgttccg
     2221 tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg aagaaaagac
     2281 cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag gattatcgca
     2341 aaggagtttg tagtgatgaa aaaacctgtc gtgatcggac tggcggtagt ggtacttgcc
     2401 gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg cctgacgctg
     2461 tatggcaacg tggatattcg tacggtgaat cttagtttcc gtgttggggg gcgcgttgaa
     2521 tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc aggtgctggg cgaactggat
     2581 cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gcgtctcggt ggcacaggcg
     2641 cagtatgacc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc cgccgcagcg
     2701 gtgaaacagg cgcaagccgc ctatgactat gcgcagaact tctataaccg ccagcaagga
     2761 ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc ctcgcgcgac
     2821 caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg ttccggtaac
     2881 cgtgaacagg acatcgctca ggcgaaagcc agcctcgaac aggcgcaggc gcaactggcg
     2941 caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg cacgctgtta
     3001 acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt taccgtttca
     3061 ctaacgcgtc cggtgtgggt gcgcgcttat gttgatgaac ggaatcttga ccaggcccag
     3121 ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta tcacgggcag
     3181 attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac gccggatctg
     3241 cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga tgcgttacgc
     3301 cagggaatgc cagtgacggt acaattcggt gacgaggcag gacatgaatg atgccgttat
     3361 cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg tcgcgccgct
     3421 cgactgtacc attcacgccg gttatgtgac ggggctggtg gggccggatg gtgcaggtaa
     3481 aaccacgcta atgcggatgc tggcgggatt actgaaaccc gacagcggca atgccacggt
     3541 gattggcttt gatccgatca aaaacgacgg cgcgctacac gccgtgctcg gctatatgcc
     3601 gcagaaattt ggtctgtatg aagatctgac ggtgatggag aacctcaatc tgtacgcgga
     3661 tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc tggagtttac
     3721 gtctcttggc ccgtttaccg gacgcctggc gggcaagctc tccggtggga tgaaacaaaa
     3781 actcggcctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg atgaacccgg
     3841 cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg agctggcggg
     3901 cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc agtgccgtga
     3961 cgtgttactg atgaacgaag gcgagctgct gtatcaggga gaaccaaaag ccctgacaca
     4021 aaccatggcc ggacgcagct ttctgatgac cagtccacac gagggcaacc gcaaactgtt
     4081 gcaacgcgcc ttgaaactgc cgcaggtcag cgacggcatg attcagggga aatcggtacg
     4141 tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg ggatgccgga
     4201 aatcaacatc aacgaaacta cgccgcgttt tgaagatgcg tttattgatt tgctgggcgg
     4261 tgccggaacc tcagaatcgc cgctgggtgc aatattgcat acggtagaag gcacacccgg
     4321 cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg ccgccaccga
     4381 tcacgtcaac tttgccgtta aacgtgggga gatttttggt ttgctggggc caaacggcgc
     4441 gggtaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt ccggccaggc
     4501 gctggtgctg gggatggatc tgaaagagag ttccggtaaa gcgcgccagc atctcggcta
     4561 tatggcgcaa aaattttcgc tctacggcaa cctgacggtc gaacagaatt tacgcttttt
     4621 ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc gtatgagcga
     4681 ggcatttggc ctgaaaagta ttgcctccca cgccaccgat gaactgccat taggttttaa
     4741 acagcggctg gcgctggcct gttcgctgat gcatgaaccg gatattctgt ttctcgacga
     4801 accgacttcc ggcgttgacc ccctcacccg ccgtgaattt tggctgcaca tcaacagcat
     4861 ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag cggaatattg
     4921 cgaccgcatc ggcctggtgt accgcgggaa attaatcgcc agcggcacgc cggacgattt
     4981 gaaagcacag tcggctaacg atgagcaacc cgatcccacc atggagcaag cctttattca
     5041 gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt tcctggcgtc
     5101 gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg agtagctggc
     5161 tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc attaacctcg
     5221 actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg gcgctggatt
     5281 tcacccacac tatgaccggt tcgccctaca tcgacgccac catcagcgat aaccgtcagg
     5341 aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt cccgtggatt
     5401 tcgccgaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc accgacggca
     5461 gtgagccgaa taccgctaac tttgtacagg ggtatgtcga agggatctgg cagatctggc
     5521 aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat gtacaaaccc
     5581 gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt gcggtgacca
     5641 tcatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga gaatgggaac
     5701 gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg ctgctgtgta
     5761 agctgatccc ctattacttt ctcgggatgc tggcgatgtt gctgtgtatg ctggtgtcag
     5821 tgtttattct cggcgtgccg tatcgcggtt cgctgctgat tctgtttttt atctccagcc
     5881 tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc cgcaaccagt
     5941 tcaatgccgc tcaggtcgcc ctgaacgccg cgttcctgcc gtcgattatg ctttccggct
     6001 ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac attattcccg
     6061 ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt ccagtggtgc
     6121 tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc ctgacgtggc
     6181 tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt atggacgtta
     6241 atccgcaaag agttgcagtc gctgctgcgc gaaccgcaaa cccgtgcgat tcttatttta
     6301 cccgtgctga ttcaggtgat cctgttcccg ttcgccgcca cgctggaagt gactaacgcc
     6361 accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac ccaacgtttt
     6421 gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga gatccgccca
     6481 accatcgaca cacaaaaggc gctactactg gtgcgtttcc cggctgattt ctcgcgcaaa
     6541 ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa ctccaacagt
     6601 gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca ggaactgctg
     6661 gaaggaaaac cgaaacccaa caacagcgag ctggtggtac gcaactggta taacccgaat
     6721 ctcgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac tatcggcgta
     6781 atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct cgatcagcta
     6841 ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc ggcattaatt
     6901 gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta tcaaatcccc
     6961 ttcgccggat cgctggcgct gttctacttt acgatggtga tttatggttt atcgctggtg
     7021 ggattcggtc tgttgatttc atcactctgt tcaacacaac agcaggcgtt tatcggcgtg
     7081 tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt ggaaaacatg
     7141 ccagtatggc tgcaaaacct gacgtgggtt aaccctattc gccactttac ggacattacc
     7201 aagcagattt atttgaagga tgctagtctg gatattgtgt ggaatagttt gtggccgcta
     7261 ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg taaggtgatg
     7321 taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga ttgccagccc
     7381 tgcaattgcc agcaaaaagt tgttttccag aacgcgggac aacagccaca gcacaatcag
     7441 cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca cctcacgcca
     7501 gcgttgctcg gggtggcgct gaaacaccag catcatcact atcgtgacac aatacagtgc
     7561 acataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca ccagcagcgc
     7621 cactaccagt aaatgcaaca taatctgcca gcaacttaga cccgttgcga cacgaacacg
     7681 ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc agagtaccga
     7741 actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca ctatttcaat
     7801 gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt ttcgttcaag
     7861 gtgctcacca tcaatattca caaaggcttt accgcgttta accgacgctt cattttgccg
     7921 gaacttcgcg atgccgtgcg taccgtcagc gccgatattg tttgcctgca ggaagtgatg
     7981 ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc gcactacgag
     8041 tttctcgccg acactatgtg gagcgatttt gcctacggtc gcaatgccgt atacccggaa
     8101 gggcatcacg gcaacgcggt tctttcacgt tatcccattg aacattatga gaatcgcgat
     8161 gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt gccgccgatg
     8221 accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc gcaccgtcag
     8281 gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga accggtattg
     8341 gtggcgggtg atttcaacga ctggcggcaa aaagctaatc atccgttaaa agtgcaggcc
     8401 gggctggatg agatatttac ccgcgcccac ggacgcccgg cgtgcacgtt tccggtgcaa
     8461 tttcctctac tacgactgga caggatctat gtcaaaaatg ccagcgccag cgcgccaacc
     8521 gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag tgcggagatt
     8581 catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa aacggcgagc
     8641 aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc attcttgaaa
     8701 cgtttatctg gtttgaggat gacgtcggca aacaactgca tgctgcacta ctggcagcag
     8761 cgcaacgcgg ggttagagcg gaagtcttgc tggatggcta cggttcgccg gatctcagcg
     8821 atgagtttgt caatgaactg acggcagctg gcgtagtgtt ccgctactac gatccccgcc
     8881 ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa attgtggtga
     8941 tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat atgtccagct
     9001 acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt gtcgaagata
     9061 ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc tggtggcgac
     9121 gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg ctggtctggc
     9181 gcgataacga agaacatcgc gatgatatcg aacgtcacta tctgaaaatg ctcactcagg
     9241 cgcggcggga agtgattatc gccaacgcct acttcttccc cggctatcga tttttacacg
     9301 ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gataattcag ggcgaaccgg
     9361 atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt aaaggcggcg
     9421 ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg atggacgatc
     9481 actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg aatctcgaag
     9541 ctaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat ctgaacggca
     9601 ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc acctggtgga
     9661 acctaaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg ctggttggct
     9721 ggcttccggc acacacgcca cgtctggcgc aggttgatcc ccccgcacaa ccgacaatgg
     9781 aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga gtaaatcaca
     9841 cccgcgctgg cgcttagcaa agaagatcct cacctggctg ttttttatcg cggtgattgt
     9901 gttgctggtg atctacgcca aaaaggtgga ctgggaagag gtctggaagg tcatccgcga
     9961 ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct atctgattta
    10021 cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga agcgccaggt
    10081 gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct gggtcggcgg
    10141 cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca ctattacgcg
    10201 gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg cagggattat
    10261 ctttaccgca ggcgtggtgg agttgccgga ccactggtat gtcgatcaaa ctacgctgcg
    10321 tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt gcgctttcgc
    10381 gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcgt ggaaattcgc
    10441 cctcgcccag atgctgattt ccagcgttaa ctggatggta atgggagcga taatctggct
    10501 gttacttggt caaagcgtga actatttctt tgtactgggc gtgttgctgg ttagtagtat
    10561 tgctggtgtc atcgtgcata ttccggcggg aattggtgtg ctggaagcgg tgtttatcgc
    10621 gctactggca ggggagcata catctaaagg cacaattatc gccgccctac tcgcttatcg
    10681 tgtgctgtat tactttatcc cactgctgct ggcgctgatc tgttacctgt tactggaaag
    10741 ccaggcgaag aagctgcggg cgaaaaatga agcggcgatg tgaagtttag ttaagttctt
    10801 tagtatgtgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga caagcgttat
    10861 agctatcgct gcaagtatcc caaagaagcc gataaaatag taatagagtg ttaatgcacc
    10921 tgtaaaagcg caacgaaccg gactccacaa ctcttcatta tggcatttcc ccgccaattc
    10981 aaggagctta tttcgtccat gcgagataat gcctgaccat aacacaatca ttaagcaact
    11041 tattacccaa atgggccgtt cactcattaa tgcggcattc acgatgatca ccagggccaa
    11101 ccctatcaac gtcatcataa tcataaaacg gtgagaacca ggatcaatat taaataataa
    11161 accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga cgataatggc
    11221 aggtacagga gctaaactag caacgacaat cgccaccaaa gagagtaaaa acagatatat
    11281 tacgcctgcg atggcggcat ttatcttgtt gatatcctta cttaagccat acatacaaag
    11341 taataataac cctatagcga catacattgt tggtgaatca tatttggttg ccgtaatttc
    11401 gagatactta gctccccagg ccagtcctgc ggttgccacc aaacctaaag cggtaatgag
    11461 ccatataccg tttaatatcc tggcctcgtg ctgaaaatct aatttattgc tgttttgtga
    11521 gtatgattcc attcttttag ttgtccttag agaaaatatt aatattcacc atcaataaat
    11581 ttaaaaccac gcatacaaaa tacttttgta tttatggata cggaaaacac ttccgctcgg
    11641 ccataaggcg tattttatac ctggtacttg ttaagtgtcg tttttagtaa gaaaaaaatc
    11701 ccgacacatg gccgggatag tgatggtgaa aaagaattaa cggcggttgc cgaagatccg
    11761 cagcaacatc aggaacaggt tgatgaaatc gagatacagc gttaatgcgc cgagaatgga
    11821 gtatttacgc aggttagagc tgtcgcgggt atcaatctgc tcacccatgt ttttcagttt
    11881 ctgggtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg tgactgccca
    11941 catcaacgct tcgcttttca gccagaagtt gaccagcgat gccaacacaa tgccgattaa
    12001 cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt aaccgtacag
    12061 gctcattgcg ccgaacatcc cggcagtaac gacgaaggta ctggcgatag aagcagcggt
    12121 ataaacaatg aaaatactgg aaagcgtaag acccgtcagc gccgaataaa gcataaagag
    12181 catcgtcgtt acacctgcgc tcagcttttg aatcatcgct gataacacaa taaccaatgc
    12241 taattgcgcg atgatcagac cgattaaaaa gacacggtta gtgaacaaca gctccatcac
    12301 ggccgcggaa ttagccgcat accaggcaat aaatgcggtc agcaacaagc caacggtcat
    12361 ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta cgattgaatc
    12421 agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc ttaaggatac
    12481 tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc atgtactgag cggtgaaatt
    12541 gccggacgca gcggtgcctt atccggctaa caaaaactac cagcgttttg ccgcctgctg
    12601 atcgctctcc cgggcttcga cccagcggtc gccttccggc gtggcttcgc gcttccagaa
    12661 cggtgcgcgg gttttgagat aatccataat aaactgcccg gcttcaaacg cactgctgcg
    12721 atgcgcactg gtgacaccga caaaaacaat ttcatcgccc ggccataatt ccccgatgcg
    12781 gtgaatcacc gtgacacgcc ccagcggcca gcggttacgc gcttcatcga caatttctgc
    12841 cagtgctttt tcagtcatcc ccggatagtg ttcgagggtt aatgctttga cgctgtcgcc
    12901 caggttatgg ttgcgcacct taccggtaaa ggtgactacc gcaccgtctt cgtcacgctc
    12961 cgccagccac gggtactctt ctcctacgct gaacggctgc ggaccaacaa caattttggt
    13021 tcctgccatc ttaacctccg gttaccggcg ggaagaaagc tacttcgtcg ccgtcagtca
    13081 gcggatggtc aaaactcacc agcgtctggt tgacggcagc cagtaatttg ccatcttcca
    13141 gcgccagcgc ccagcgatcg ctctgcgcag ccaggtgctg gcgtaacgct tcaacggttg
    13201 ggaaatccgc agccacttcg gttgcatctg ttcctaccaa ctcgcgcacc tgggcgaaaa
    13261 aaagaacttt aatcatccgc ttccacctta aagtcacccg acttgccgcc gctcttcgcc
    13321 agcaaacgta ccggaccaat caccatatct ttttgcaccg ctttgcacat gtcataaatg
    13381 gtcagcgccg ccacggaggc cgcggttaac gcttccattt cgacaccggt tttcccggtc
    13441 aggcggcata aggtttctat acgcacccga ttgtgctccg gctcggcctg taaattgact
    13501 tcaactttgc tgagcatcag cggatgacac agcgggatca gatcccacgt gcgttttgcc
    13561 gcctgaatac cggcaatacg cgcagtggca aatacgtcgc ctttgtggtg gcgaccatca
    13621 ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg cttcggcgcg cgcttcacgc
    13681 acggtttccg ctttggcgga gacatccacc atgtgcgctt cgccagcggc gttgatatgg
    13741 gtcagttgcg acatacttat ttcttcaaat gtggatggaa attacacgga cgcgtacggg
    13801 catccagctg cggcgcgatg atattttccc atgcggtacg gcacgctttg gtcgaacccg
    13861 gcatggcgaa aatcagcgtt ttgttagcga cgcccgctac cgcacgagat tgcaacgtgg
    13921 aagtgccaat ctcttcaaac gacaacatac ggaacacttc accaaaacct tcaacttcac
    13981 ggtcgaacaa cggcagcaat gcttcgggag cctgatcacc ttccgtcagg ccagtacccc
    14041 ccgtaatcaa caccacttgt acatcgtcgc tggcgatcca cgcagatacc tgagcgcgaa
    14101 tagcgtagcg gttttctttc acaatggctt tatcgacaac gtgatggccc gcttcttgcg
    14161 ccgaatcgcg caaatagtga ccggaggtat cgtcttcttc accgcgccga ttagaaaccg
    14221 taagaatagc aatacgggtc gggataaatt cagtgcttac ctgactcatc tgatctctcc
    14281 ttttgacgtt ttagccgcca atgtacgata agttttgtgt aataccggtg ttgttttgat
    14341 gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga aatacgcgct tccagcgcct
    14401 gttgctgggt atcgtcttcc agcagatcgc gcaggttaac gccgccttca ccaaacaggc
    14461 agagatggag tttaccaatc gaggaaacgc gcaggcggtt gcaagtggcg cagaagtctt
    14521 tttcatacgg catgataagg ccaatctctc cggcgtaatc cggatggcaa aagacttgcg
    14581 cgggaccgtc gctgcgttga cgtaattggt ggatccagcc gcgacgcagt agctcgtcac
    14641 gcagaacctg accagagatg tgatgcttac ggaagagctc actgccctcg cccgtttcca
    14701 tcagttcgat gaaacgcagc tggatgggac gatgctggat ccagttcaga aaggtgtcaa
    14761 gctggtgatg attaacatca cgcatcagca cggtattgac tttgaccttc tcaaaaccgg
    14821 cctcaaatgc agcatcaatc cctgccatga cctggttgaa tttgtcctgc ccggtaatag
    14881 cgtgaaactg gcgggcgtcc agactgtcga cactgacgtt aatgccagta agtcccgcat
    14941 cgcgccagtt cgccacatcg cgttccagac ggtaaccatt ggtggtgaca gcaatctggc
    15001 ggatagcgtc gttttctcgc acagcggcga tgatatcggt aaagtcgcgg cgtaaagacg
    15061 gctcaccgcc tgtcaggcgc actttttcgg tgcccagact ggcgaaggcg cgcgtaaccc
    15121 ggcgaatttc atcgacggtc agaaagcctt tattggtgac gccgctcggt ttgtagccat
    15181 ccggcaggca gtaggtgcaa cgaaagttac acacatcggt aatcgacagg cgcaagtagt
    15241 aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat gtacaccttt ccagatacgg
    15301 gaggcgaagt catttcttcc ttcgccctgg tggcaatctt tacgagcatt gtcacggcca
    15361 aagcaccgta tcagttgacc caggtgcaga ggctagagtg tttagtggtt aagccgatac
    15421 tagcgtgtaa acgagatttt taccatccac actttcgcta tgcaatcatg tatatagcgt
    15481 catgatcgtg ctatttcgct acagaataag ggaaaatggc ggttttactt tgatatacat
    15541 catcagggag gcatagcgtg catcgtcctt tagaggtaga agaaaatagt ttgtatcctc
    15601 aatattggca ggttaattgc tgtttccccg caatttgcgc tactgtagcg cggataatct
    15661 gactccagga aactatatgc gcaatcgtac gctggctgat cttgatcgtg tcgttgctct
    15721 cggcggaggg catggactgg gacgcgttct ctcatcactt tcgtctttgg gttctcgttt
    15781 aacgggtatc gtcaccacca ccgataacgg tggctcgacg gggcgtattc gccgttcaga
    15841 aggcggcatt gcctggggcg atatgcgcaa ctgcctcaac caactgataa cggaaccgag
    15901 cgtcgcctcc gcgatgtttg aataccgttt tggcggcaat ggcgaacttt ccggtcataa
    15961 tctcggaaac ttgatgttaa aggcgctgga tcaccttagc gtgcggcctc tggaagccat
    16021 caatttaatt cgtaatctgc tgaaagtgga tacgcatttg attccaatgt cagagcatcc
    16081 tgttgatctg atggcaattg acgatcaggg gcatgaagtt tacggcgagg tcaatatcga
    16141 ccagttaact acgccgattc aagagttatt gttaacgcct aatgtacccg caacgcgtga
    16201 ggcggttcac gctatcaatg aagcggatct catcattatt gggcctggca gtttttatac
    16261 cagcctgatg ccaattctgc tgctgaagga aatcgcgcag gcattacgcc gcacgccagc
    16321 gccgatggtc tatatcggca atctggggcg tgagttgagt ttacctgcgg ccaatttgaa
    16381 gctggaaagc aagctggcaa ttatggagca gtatgttggt aaaaaggtca ttgatgcggt
    16441 catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag cggattgtga ttcaggaggt
    16501 gctggaggcc agcgatattc cgtatcgtca tgaccgtcag ttgttacata gcgcgctgga
    16561 aaaggcatta caggctttag gttaacggaa gtccgaagga aaattccggc ttcctattga
    16621 agacaaagtg cgcgttgttt atgccggatg cggcgtgaac gccttatccg gcctacaaac
    16681 cgcgctaatt caatgtattg cagagatcat gtaggcctga taagcgtagc acatcaggca
    16741 attttgcttt tgtcatctgt cttcttcgtt atcctgtcac gaagccgcaa tgaacaattc
    16801 acgcagctga tgcaactggt cacgaatttg cgccgcttct tcgaactcca gattctgcgc
    16861 gtgttgcatc atcaaccctt ccagttcatg gattttctgc tgcagcgctt taggcgacat
    16921 atccatcggc acattatccg gctcaacaat cgggcgcgat tttcctctgc ccttcgcttt
    16981 ggttttagca atgttctgcc ccaacgccag gatatcgacc actttcttgt tcaagccttg
    17041 cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc tcgcggcgac gttcggtttc
    17101 gccaatcgct ttcgccattg atggggtgat cttatcgccg tagagaatcg ctttaccgtt
    17161 aacgttacgt gccgcacgac caatggtctg gatcaacgaa cgttcggaac gcaggaagcc
    17221 ttctttgtca gcgtcgagga tcgccaccag cgaaacttcc ggcatatcca gaccttcacg
    17281 cagtaagttg atccctacca atacgtcgaa ctcacccaga cgcaagtcgc ggataatctc
    17341 catacgctcg acggtgtcga tatctgagtg aagataacgc acgcgctcac cgtgttcttc
    17401 gagatattcg gtaagatctt ccgccattcg cttggtcagt gtggtgacca gtacgcgttc
    17461 gttaattgcc gctcgctgac gaatctccga aagaagatca tcaacctgtg tcgccactgg
    17521 ccgcacttcg ataatcggat caagcaatcc ggttggacgc accacctgat ccaccacatc
    17581 gccgccggat ttttccagct cataattacc tggcgtcgcc gaaacataga tggtttgcgg
    17641 cgctaatgct tcgaactctt caaatttcag cgggcggtta tccagcgctg atggcaggcg
    17701 gaagccgtac tccaccagtg tctctttacg cgcccggtca ccgcgataca tgccgccaat
    17761 ttgtggaatg gtgacgtgag attcatcgac caccagcagc ccatcggcag gcaggtaatc
    17821 aaacagcgtc ggcggtggct cacccggtcc acgaccggag aggaagcgcg agtagttttc
    17881 aatccccgaa cagtagccca gctcgttcat catctccaga tcaaactggg tacgctgggt
    17941 cagccgttgc tcttccagca gtttgttgtt ttccaatagc actttgcgtc tggcggccag
    18001 ctcttctttg atctcctcca tcgcctgtac gatgcgctcg cgcggtgtga cgtagtgcgt
    18061 tttcgggtag atggtaaaac gtggaatggt ggaaacaatc tgtccggtca gcgggtcaaa
    18121 taacgacaat cgttccactt cctcgtcaaa cagttccacg cgaagtgcaa tgtcatccga
    18181 ttctgccggg aagatatcaa tcacctcgcc acgaacgcgg aaagtaccac gctggaatgc
    18241 ttgatcatta cgagcgtatt gcagctccgc caatcggcgc agaatcgcgc gctgatcgat
    18301 aatcataccg accgtgagat ggagcatcat cttgagatat aaatcaggat cgcccagacc
    18361 ataaatcgcg gaaacagacg ccaccacaac cacatcacgt cgctccagca tcgctttggt
    18421 ggcggacaaa cgcatctgtt cgatatgttc gtttaccgag gcatctttct caatgaaggt
    18481 gtcagaactc ggtacatagg cttccggctg atagtagtcg tagtaggaga cgaaatactc
    18541 caccgcgttt tccgggaaga actctttcat ttcgccatac agctgggccg ccagcgtttt
    18601 gttgggcgca agtaccatgg ttgggcgctg aaggtcagca atgacattgg caatggtgaa
    18661 ggttttcccg gagccagtca cgccaagtaa cgtctggtgc gccaggccat cttccagccc
    18721 ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa ggtttaaaag cggaattcag
    18781 tttgaacggt ttactcatga gtctctacct gaaggagttg ggcgggcagg tatgtaattt
    18841 tactcgtcgt acttaattat gccaacaaat tatactggat aaaaaaacag ttcatcacca
    18901 taatatttct gatacagcgt aaactccgct gtcaatcatg agcaaaattt actctgtggc
    18961 gagatacaac tccggcctta ccgggttatc cccaaagcaa cggctttttt aacatttgtc
    19021 aagatgagtg atgacagttt tatggcaaga gatgcctcgt tagtgactgc cattgcaatt
    19081 atctaaccag ttaaaaaata aaagaaattt ctttgagccg tctttaacgc caattcgcgt
    19141 gcaagccgcg tattctctcg cttgcctcgt gttttctaac tctaatacac atggttatcc
    19201 acaggaatag tggataactg tctgcagccc ctatcccccg ccgcctgggc aattcccaca
    19261 ttcaccgcat gatgtggagg taaaattttt ttcgtgatta ttttagaatt taattggtta
    19321 aattgcagtc aaccgaagac tcgatctcgc tcgcaattta accaaataca ggatggctac
    19381 aacaaggcaa ggtttatgta ctttccggtt gccgcatttt ctggattttc tgcaagccag
    19441 gggatctctc ccagcagcgg cgcgggaatc atgcgggtga gcgtggtcat atattcagcg
    19501 tgacgttttc ccggaggcgt gacatcgttc gccacccaac ccgccagcgt cagtccggcg
    19561 tgttgtattg cctgtgcagt caacatcgcg tgattaatac agccgagttt cacgccaacc
    19621 accagtatta ccggcagttg ttcctgtgtt acccaatccg caaaagtgaa agtgtcagaa
    19681 aggggcgtaa accagccgcc agcgccttcc actaacaccc agtcagcctg ttgttcaagc
    19741 gcgcgtaatc cggcgctcat taccgatgat tctatcggcc tgccctcttg cgcgctgatg
    19801 atgtgcggtg aggtgggttc tgcgaaggtg taaggattta ccgttgcgta atccagctgc
    19861 aggctgctgt tgcgctgtaa cgccagcgcg tcactattac gtaaaccctc cggggccttt
    19921 tcgctgccag aggcgaccgg tttataacct gccgtccgat aacctactgc ctttgcggct
    19981 tgtaaaagtg cacaactggc gacagttttc cccacttcgg tatccgttcc ggtgacaaaa
    20041 taacgtttac tcacgaacaa tcactcccaa aaaaagatga tacgtcagag gatatcgccc
    20101 ctgttgttgc ggccaggcca gttgcaatcg ctgcaactgt gaacgcgtta atattcgctg
    20161 gtcgcgccct tcatgaagat gcgtggcacc gatgcctttc agcgaacgca tggcactgag
    20221 cgcatcatca aaccatagtg tgatgggctg aatatgatgt tgataatgca cgccgttcag
    20281 cgactgttcg atttcatctg gcggtaaaaa gcgattagca tgcgggcgct cgtccaccgc
    20341 ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc agcgtggtaa acgcgaccac
    20401 gcctccgggg cgcaccaccc gatacagctc gcggagtgcc gttgataaat taccgcacca
    20461 ctgcaccgcg agattgctcc atgcaagatc gaacgtcgca gtcgctaacg gcagggattc
    20521 gatatctccc gccagataat gatctgcggc atctttatgg cgggcttgcg caagcattgg
    20581 cggcgagaga tctaacgccg tcacctgcgc gtgacgctct cgccagtggc ggctcatcca
    20641 gcctggccca caccccgcgt ccagtacgtg ggtgtattta cgctgtggga gcattgccag
    20701 taagacgtca gcactctggc gctgtaactc tgcgtgttgc tcatagtgcg cggctgcccg
    20761 accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc atgcagcacc tccagcagac
    20821 ggtcgatatc ctgcatttca tgcgcggcgg ttagcgttaa gcgcagtcgc gcagtaccag
    20881 agggtacggt tggcggacga atcgccgtga cccagcagcc ttgctggcgc agtttttctg
    20941 ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg ctggatggcg ctgcatgaat
    21001 cagccagcgt aaacggcaaa tcctgtactc cggcacgaaa acgcgtaatg agtgccgcca
    21061 gtttttcgcg ccgtgcatca ccctcatcac tgcgaatgac cgccagtgac gcacgtaatg
    21121 cctgcgcctg agcgggcggc atactggtgc tgtagataag atggcgggcg aattgcagca
    21181 gataatccgc caccgtactg gagcaaagca ccgctgcccc gctgacgcca aatcctttgc
    21241 caaaagtcac taccagcaat tctggtttta ccttttgcag ccagcagctg ccgcgcccct
    21301 gctccccgat aacgcccgtg ccgtgggcat catcgaccat caaccagcca ttgtgctgtt
    21361 gcgttacctg ctggatttcc gccagtggcg cactatcgcc gtccatgctg aacacgcctt
    21421 ctgtcaccac cagttgctgc cccggacagg gggaagcaag cagtcgcgcc agatgagcga
    21481 catcgttatg gataaaacgg cgaagctgcg acgggcttaa actggcagcc tccagcaatg
    21541 aggcatggct aagccggtcg gcaacaatac ggtcctcttt cgccatcatc gcggcaatga
    21601 ccgcctgatt agcggcaaaa ccagagataa acagcagtgc ccgcgaatag cccagccact
    21661 cggccagttc ttcttccagc gcctgatgtg ccacgctata accgctgacg tgaccggaac
    21721 cgccgctacc gacgccaaat tgctccgcac cctgctgcca ggcacggata atttgcggat
    21781 gatggcttaa acctaaataa tcgttactgg aaaagttcag atactggcga tcgtccgcca
    21841 ccagccagcg tccggctcct tgcgccaccg gataacggcg acgcagggca tcggcagcac
    21901 gccgcgcatc gagcgccgcg ttgattttct cctgccagct catagtgctg ccgcgttgta
    21961 atattcgtca gtatccgggg tcatcagtgc ctgctccaga cgctgctgtt gttcgttatc
    22021 ccctgccagc acggcacttt gctgcggatt taaccccagt ttgcgaaaca gttgcaggtc
    22081 tttatcttct tccggattcg gcgtagtcag cagtttgcag ccgtagaaaa tcgagtttgc
    22141 gccagccata aagcacattg cctgtgtctg ttcattcatc tgctcacgtc cggcagaaag
    22201 gcgtacgtaa gaggtcggca tcatgatccg cgctacggca atggtgcgaa taaaatcaaa
    22261 ggcatcgaca tcatcgttat ccgccagcgg cgtgcctttc accttcacca gcatgttaat
    22321 tggtacgctt tccggcggcg tcggcaggtt tgccagttgc agcaataatc cggcgcgatc
    22381 ttttaccgtt tcgcctaagc caacaatgcc accagaacag actttgatcc cggcatcgcg
    22441 cactttttcc agcgtatcga ggcgttcctg ataagtgcgc gtggtgatga tattgccgta
    22501 aaactccggc gaggtgtcga ggttgtggtt gtagtaatcc agcccggcgt tcgcgaggcg
    22561 ctgcgcctga gattcactca acgtgcccag cgtcatacac gcctccagcc ccatcgcttt
    22621 taccccctgc accatttgtt ccaggtacgg catatcgcgt tcgtggggat tcttccacgc
    22681 cgcgcccata cagaagcgcg tcgatcctgc cgctttcgct ttgcgcgccg actccagcac
    22741 ctgttcaact tccatcaacc gctcggcttc cagcccggtt ttgtagcgcg agctttgcgg
    22801 gcagtatttg caatcttccg gacaagctcc ggtcttaatc gacagcaacg tgctgacctg
    22861 cacctgacga ggatcgaaat gctggcgatg cacctgctgc gcttcaaaca gcagatccag
    22921 caacggtttt tcaaataatt ctgtgacttg cgacaatgtc cagcgtgggc ggtgagccat
    22981 ggggcttctc caaaacgtgt tttttgttgt taattcggtg tagacttgta aacctaaatc
    23041 ttttcaattt ggtttacaag tcgattatga caacggacga tcttgccttt gaccaacgcc
    23101 atatctggca cccatacaca tccatgacct cccctctgcc ggtttatccg gtggcgagcg
    23161 ccgaaggttg cgagctaatt ttgtctgacg gcagacgcct ggttgacggt atgtcgtcct
    23221 ggtgggcggc gatccacggc tacaatcacc cgcagcttaa tgtggcgatg aagacgcaaa
    23281 ttgatgccat gtcgcatgtg atgtttggcg gtatcactca tgcgccagcc attgagctgt
    23341 gccgcaaact ggtggcgatg acgccgcaac cgctggagtg cgtttttctc gcggactccg
    23401 gttccgtagc ggtggaagtg gcgatgaaaa tggcgttgca gtactggcaa gccaaaggcg
    23461 aagcgcgcca gcgttttctg accttccgca atggttatca tggcgatacc tttggcgcga
    23521 tgtcggtgtg cgatccggac aactcaatgc acagcctgtg gaaaggctat ctgccagaaa
    23581 acttgtttgc tcccgccccg caaagccgca tggatggtga atgggatgag cgcgatatgg
    23641 tgggctttgc ccgcctgatg gcggcgcatc gtcatgaaat cgcggcggtg atcattgaac
    23701 cgattgtcca gggcgcaggc gggatgcgca tctaccatcc ggaatggtta aaacgaatcc
    23761 gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga cgagatcgcc accggatttg
    23821 gtcgtaccgg caaactgttt gcctgtgaac atgcagaaat cgcgccggac attttgtgcc
    23881 tcggtaaagc cttaaccggc ggcacaatga ccctttccgc cacgctcacc acgcgcgagg
    23941 ttgcggaaac catcagtaac ggcgaagccg gttgctttat gcatgggcca acttttatgg
    24001 gcaatccgct ggcctgcgcg gcagcaaacg ccagcctggc gattctcgaa tctggcgact
    24061 ggcagcagca ggtggcggct attgaagtac agctgcgcga gcaacttgcc cccgcccgtg
    24121 atgccgaaat ggttgccgat gtgcgcgtgc tgggggcaat cggcgtagtc gaaacaactc
    24181 gtccggtgaa tatggcggcg ctgcaaaaat tctttgtcga acagggtgtc tggatccggc
    24241 cttttggcaa gctgatttac ctgatgccgc cctatattat tctcccgcaa cagttgcagc
    24301 gtctgaccgc agcggttaac cgtgcggtgc aggatgaaac atttttttgc caataacggg
    24361 aagtcagcgt gagggtttct ggctacactt tctgcaaact agaaaggagg gttcatgaaa
    24421 ctcatcagta atgatctgcg cgatggcgat aagttgccac atcgtcatgt ctttaacggc
    24481 atgggttacg atggcgataa tatttcaccg catctggcgt gggatgatgt tcctgcggga
    24541 acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc caaccggctc cggctggtgg
    24601 cactgggtag ttgttaattt acccgctgat acccgcgtat taccgcaagg gtttggctct
    24661 ggtctggtag caatgccaga cggcgttttg cagacgcgta ccgactttgg taaaaccggg
    24721 tacgatggtg cagcgccgcc gaaaggcgaa acccatcgct acatttttac cgttcacgcg
    24781 ctggatgtag agcgtattga tgtcgatgaa ggagccagcg gcgcgatggt cgggtttaac
    24841 gttcatttcc actctctggc aagcgcatcg attaccgcga tgttcagtta attactctgc
    24901 cagatggcgc aatgctatct ggtatcactt aaaggtatta aaaacaactt tttgtctttt
    24961 taccttcccg tttcgctcaa gttagtataa aaaagctgaa cgtgaaacgc taaagaccat
    25021 taatatcaat gtgttacaat atatttagtc taaaaaatag actgcataat actacaaaac
    25081 acaacatatc tagtaactat gaatcaacta cttagatagt attagtgacc tgagacagag
    25141 cattagcgca aggtgatttt atgtcttctt gcgctaattt tttgtcataa aaac
//
LOCUS       DAZUGK010000048        25084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZUGK010000048 DAZUGK010000000
VERSION     DAZUGK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.048"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..88)
                     /locus_tag="ACHY5W_001754"
     rRNA            complement(<1..88)
                     /locus_tag="ACHY5W_001754"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(532..3105)
                     /gene="clpB"
                     /locus_tag="ACHY5W_001755"
     CDS             complement(532..3105)
                     /gene="clpB"
                     /locus_tag="ACHY5W_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4446665.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3235..3966)
                     /gene="yfiH"
                     /locus_tag="ACHY5W_001756"
     CDS             complement(3235..3966)
                     /gene="yfiH"
                     /locus_tag="ACHY5W_001756"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFS4446666.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3963..4943)
                     /gene="rluD"
                     /locus_tag="ACHY5W_001757"
     CDS             complement(3963..4943)
                     /gene="rluD"
                     /locus_tag="ACHY5W_001757"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFS4446667.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            5078..5815
                     /gene="bamD"
                     /locus_tag="ACHY5W_001758"
     CDS             5078..5815
                     /gene="bamD"
                     /locus_tag="ACHY5W_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFS4446668.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            6086..6427
                     /gene="raiA"
                     /locus_tag="ACHY5W_001759"
     CDS             6086..6427
                     /gene="raiA"
                     /locus_tag="ACHY5W_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4446669.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6530..6655
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6531..6578
                     /gene="pheL"
                     /locus_tag="ACHY5W_001760"
     CDS             6531..6578
                     /gene="pheL"
                     /locus_tag="ACHY5W_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFS4446670.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6677..7837
                     /gene="pheA"
                     /locus_tag="ACHY5W_001761"
     CDS             6677..7837
                     /gene="pheA"
                     /locus_tag="ACHY5W_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFS4446671.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLQSAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7880..9001)
                     /gene="tyrA"
                     /locus_tag="ACHY5W_001762"
     CDS             complement(7880..9001)
                     /gene="tyrA"
                     /locus_tag="ACHY5W_001762"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFS4446672.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(9012..10082)
                     /gene="aroF"
                     /locus_tag="ACHY5W_001763"
     CDS             complement(9012..10082)
                     /gene="aroF"
                     /locus_tag="ACHY5W_001763"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFS4446673.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10292..10657
                     /gene="yfiL"
                     /locus_tag="ACHY5W_001764"
     CDS             10292..10657
                     /gene="yfiL"
                     /locus_tag="ACHY5W_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFS4446674.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10807..11325
                     /gene="yfiR"
                     /locus_tag="ACHY5W_001765"
     CDS             10807..11325
                     /gene="yfiR"
                     /locus_tag="ACHY5W_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFS4446675.1"
                     /translation="MRFSHRLFLLLILLLTGAPIFAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKKDG"
     gene            11318..12541
                     /gene="dgcN"
                     /locus_tag="ACHY5W_001766"
     CDS             11318..12541
                     /gene="dgcN"
                     /locus_tag="ACHY5W_001766"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFS4446676.1"
                     /translation="MDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLTL
                     KQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDNQQNIIA
                     SWHYTAREPGDTFSNFISRWLFPAPVIQPIRHNGETIGEVQLTARDSSISHFIWFSLA
                     VLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHR
                     FALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNSADAR
                     KTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVL
                     YDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADH
                     NMYQAKHQRAEKLVR"
     gene            12557..13039
                     /gene="yfiB"
                     /locus_tag="ACHY5W_001767"
     CDS             12557..13039
                     /gene="yfiB"
                     /locus_tag="ACHY5W_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS4446677.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(13116..13463)
                     /gene="rplS"
                     /locus_tag="ACHY5W_001768"
     CDS             complement(13116..13463)
                     /gene="rplS"
                     /locus_tag="ACHY5W_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4446678.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13505..14272)
                     /gene="trmD"
                     /locus_tag="ACHY5W_001769"
     CDS             complement(13505..14272)
                     /gene="trmD"
                     /locus_tag="ACHY5W_001769"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4446679.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14303..14851)
                     /gene="rimM"
                     /locus_tag="ACHY5W_001770"
     CDS             complement(14303..14851)
                     /gene="rimM"
                     /locus_tag="ACHY5W_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4446680.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14870..15118)
                     /gene="rpsP"
                     /locus_tag="ACHY5W_001771"
     CDS             complement(14870..15118)
                     /gene="rpsP"
                     /locus_tag="ACHY5W_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4446681.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15255..16616)
                     /gene="ffh"
                     /locus_tag="ACHY5W_001772"
     CDS             complement(15255..16616)
                     /gene="ffh"
                     /locus_tag="ACHY5W_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4446682.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16783..17574
                     /gene="ypjD"
                     /locus_tag="ACHY5W_001773"
     CDS             16783..17574
                     /gene="ypjD"
                     /locus_tag="ACHY5W_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFS4446683.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17595..18881
                     /gene="yfjD"
                     /locus_tag="ACHY5W_001774"
     CDS             17595..18881
                     /gene="yfjD"
                     /locus_tag="ACHY5W_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFS4446684.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18936..19529)
                     /gene="grpE"
                     /locus_tag="ACHY5W_001775"
     CDS             complement(18936..19529)
                     /gene="grpE"
                     /locus_tag="ACHY5W_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4446685.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19651..20529
                     /gene="nadK"
                     /locus_tag="ACHY5W_001776"
     CDS             19651..20529
                     /gene="nadK"
                     /locus_tag="ACHY5W_001776"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFS4446686.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20615..22276
                     /gene="recN"
                     /locus_tag="ACHY5W_001777"
     CDS             20615..22276
                     /gene="recN"
                     /locus_tag="ACHY5W_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4446687.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22425..22766
                     /gene="bamE"
                     /locus_tag="ACHY5W_001778"
     CDS             22425..22766
                     /gene="bamE"
                     /locus_tag="ACHY5W_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFS4446688.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22828..23118)
                     /gene="yfjF"
                     /locus_tag="ACHY5W_001779"
     CDS             complement(22828..23118)
                     /gene="yfjF"
                     /locus_tag="ACHY5W_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFS4446689.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(23108..23545)
                     /gene="ratA"
                     /locus_tag="ACHY5W_001780"
     CDS             complement(23108..23545)
                     /gene="ratA"
                     /locus_tag="ACHY5W_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFS4446690.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23716..24198
                     /gene="smpB"
                     /locus_tag="ACHY5W_001781"
     CDS             23716..24198
                     /gene="smpB"
                     /locus_tag="ACHY5W_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4446691.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24413..24775
                     /gene="ssrA"
                     /locus_tag="ACHY5W_001782"
     tmRNA           24413..24775
                     /gene="ssrA"
                     /locus_tag="ACHY5W_001782"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6390 a         6628 c         5866 g         6200 t
ORIGIN      
        1 agcaagctgc ttcctgttac cgttcgactt gcatgtgtta ggcctgccgc cagcgttcaa
       61 tctgagccat gatcaaactc ttcaatttaa aagtttgatg ctcaaagaat taaacttcgt
      121 aatgaattac gtgttcactc ttgagacttg gtattcattt ttcgtcttgc gacgttaaga
      181 atccgtatct tcgagtgccc acacagattg tctgataaat tgttaaagag cagtgccgct
      241 tcgctttttc tcagcggcgc ggggtgtgca taatacgctt tcccgctaca gagtcaagca
      301 tttatttttg ctttctctgc tgaagttctc aggagaaccc cgctgacccg gcggcgtgtt
      361 tgccgttgtt ccgtgtcagt ggtggcgcat tatagggagt tattccggcc tgacaagcga
      421 aaaatataaa aactttatcg ttcgctcatt tttcaggcaa aacatcttaa atatagtctt
      481 ttccgtctaa cttatagaca aaaacgagcc ccgaagggct cgttttatca tttactggac
      541 ggcgacaatc cggtcttcat taacttccag gcgaatcact ttacccggaa ccaattcacc
      601 agacagtatt tgctgtgcca gcgggttttc gatctgctgc tgaattgcac gtttcagagg
      661 acgtgcacca tagaccggat cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga
      721 aatgtggatt tcataaccac gttcttccag acgtttatac agacgtttca attgaatctg
      781 cgcaatcgac gcaatgtgct gttcacccag cggatggaag accaccactt catcgatacg
      841 gttaatgaat tccggacgga agttatggct aaccacaccg agcaccagct ctttcatgtg
      901 cgcataatcc agttcaccga agcgttcctg aatcagatcg gaaccgaggt tagaggtcat
      961 aatgacaacc gtattacgga agtcgaccgt tctcccctgc ccgtcagtca gacgcccatc
     1021 atccagtacc tgcaacagaa tattgaagac atccggatgc gctttttcca cttcatccag
     1081 caggatgacg gaatacggac gacgacgcac agcttcggtc aggtagccac cttcttcata
     1141 accgacatat cccggaggtg caccaaccag acgagacacc gagtgtttct ccataaactc
     1201 ggacatatcg atacggacca tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt
     1261 acaaagctct gttttcccca caccagttgg gccgaggaac aggaatgaac caatcgggcg
     1321 atttggatcc gccagccccg cacggctacg acgaatagcg ttagataccg catcaaccgc
     1381 ttcgttctga ccaattacgc gatggtgcag ttcttgctcc atacgcagca gtttttcgcg
     1441 ctcgctttcc atcatgcgag aaaccggaat ccccgtccaa cgcgccagca cttcagcaat
     1501 ttcggcgtcg gtcactttat tacgcaacag acgcatagtt ttgccttcga gctgcgttgc
     1561 ggcttccagt tgcttttcca gttccgggat tttgccgtat tgcagttcag acatccgcgc
     1621 caggtccccc acacggcgag cctgttcaat agcgattttc gcctgttcca gttccgcttt
     1681 aatggtctgc gtaccagaaa gcgatgcctt ctctgctttc cactcttctt ctaactcgga
     1741 gtactgacgt tctttgtcgc tcagttcttc gttgagcata tccagacgtt ttttactggc
     1801 ttcatcagac tctttcatta acgcctgttg ttccagtttg agctggatga tacggcgatc
     1861 gagtcggtcg agttcttctg gttttgagtc aatctgcata cgaatgctgg atgctgcttc
     1921 atcgatcagg tcgatggctt tatccggcag ctgacggtca gcaatatagc gatgagacaa
     1981 cgtcgccgct gcaacaattg ccgggtcagt aatttgcaca tggtggtgca attcgtaacg
     2041 ttctttcagg ccacgcagaa tcgcaatggt atcttcaaca gaaggctcgg caacaaacac
     2101 tttctggaaa cgacgttcca gcgcagcatc tttttcgatg tactggcgat attcgtcaag
     2161 cgtcgtggca cctacgcagt gcaattcacc acgcgccagc gccggtttca gcatgtttcc
     2221 ggcgtccatt gcgccatcgg ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa
     2281 caggatgacg ttgccttcct gtttggcaag atcgttaagc acgcctttta aacgttcttc
     2341 aaactcaccg cgatatttcg ccccagccac cagcgcgccc atatccagcg ccagtacccg
     2401 gcggcctttc aacccttccg gcacttcgcc gttgataata cgctgcgcca gaccttcaac
     2461 gatggcagtt ttaccgacgc cgggttcacc aatcagtacc gggttatttt tagtacgacg
     2521 ttgcagcacc tgaatggtac ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt
     2581 gccctgttcg gctcgttcgg taaggtcgat ggtatatttt ttcaaagcct gacgttggtc
     2641 ttcagcacct tgatcgttca cgctttcacc tccacgcatt tgttcaatcg cttgagtaat
     2701 gttggcggtg gtcgcccctg ctgctttcag gatgtcggcc agcgtgccgc gagactcaag
     2761 tgccgccaga acgaacagtt ctgacgagat aaagttatca ccacgttttt gcgccagctt
     2821 gtcgcaaaga ttaagaacgc gcaccagatc ctgtgatggc tggacatcac caccagtacc
     2881 ttcaacctgc ggtaaacgat ttaatgcctg attgatatct gtgcgcaact ggccagcatt
     2941 tatgccagcg gatgttaata aaggactaac cgaaccccct tcctgattca gcagggcgct
     3001 cattaaatga agtggttcga taaattggtt gtcgtgcccg agtgcaagtg attgggcatc
     3061 ggcaagagca agctggaatt tattagtaag acgatccaga cgcataactc ctcccataac
     3121 ggatcaaagt tgctactgga gattaaatga ggtcatccct caattattca aggttattga
     3181 ccagattaat gtgaaaagaa aatcacgcgt accggatcgt cttgattctt taggttatat
     3241 cagccaaatg aaacttgcca tacgcccggt ggtcttgtcg cgacgataag agaagaaagt
     3301 ctcattttcc gtatatgtac aacggtcgcc gccgaaaatt tgttcaacac caacgctcgc
     3361 cagacgctgc cgggcaagca gataaatatc cgccagatac ttatcaccat gctgaatgaa
     3421 agctgtactc gctttggcgt ctgctgccat aaacgcctcg cgaacctccg cccccacttc
     3481 gaacgcgcgt ggaccaattg ccggccctaa ccaggcgaga atattttccg gattatcagc
     3541 aaaacaggaa actgtctctt ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc
     3601 ggcggcgact tccgttcccg ctcgattgca aaacagcaca gggaggcagt cggcagtcat
     3661 cactgcgcaa accgtgccgg gcgtattgct ataagaggca tctgctcgtt ttgaggcata
     3721 aggttcgcca gtaagattaa gcacatcttt gccgtgtacc tgctcaagcc agaccggttt
     3781 agaaggcaaa ttgcccgcag caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt
     3841 atcgccacaa tgggcaccga ggttgagcga gtcatacggg ggcaagctca cgccgccgat
     3901 acgagtagag ctacaagccg caacaccttt tggcagcggc cactgcggga caatcagctt
     3961 actcataacc agtccacttc atccttatgt tcttcgaaat cggcacgcat cgcctcaatc
     4021 agctccacca tatcttgtgg aataggcgca tgccattcca tttcgatgcc ggagatcggg
     4081 tgataaagac gcagcatggt tgcatgaagc gcctggcggt caaacttacg cagcgtggag
     4141 ataaacgctt ccgaagcacc ttttggcgga cgcggacggc caccataaac cggatcgccc
     4201 accagcggat gagtgatatg ggccatatgc acgcggatct ggtgcgtacg tccagtttcc
     4261 agacgcaacc gcagacgcgt gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc
     4321 gctggtttgc ccatcggatg caccgccatg tgagtacgtt tggtcgggtg gcgactgatt
     4381 ggctcatcca ccgtaccgcc agcggtcata tgaccaattg ccaccgcttc atactcacga
     4441 gtaatttcac gcctttgcaa agattcgact aaacgcgtct gagccggaac ggtttttgcc
     4501 acaaccatca ggccagtggt gtctttatcc agacgatgga cgatgcctgc acgcggtaca
     4561 tcagcaatgg gcggatagta atgaagtaac gcattcagta ccgtgccatc cgggttaccc
     4621 gcgccaggat gtaccaccag atcgcgcggt ttattaatga cgatgatatc ttcatcttca
     4681 tagacgatat ccagcgggat atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg
     4741 ttgatggcaa cctgctcgcc acccaatact ttttctttcg gcttatcaca aactttgccg
     4801 tttaccagca cgcgctggtc gaggatccat tcttttattc gcgaacgtga ataatccggg
     4861 aacatttcgg ccaaagcctg atctaagcgt tgaccgagtt ggttttcgga caccgttgca
     4921 gtgagctgta ctcgttgtgc catatatact gcttcttcgt ttatcgttgg ggttttacgg
     4981 ctttgccgtt taatatagtg tgctattgta gctggtctta accgggagca ggaacagaga
     5041 atctcccgta ttacattttg aggaaagtca aaacgtcatg acgcgcatga aatatctggt
     5101 ggcagccgcc acactaagcc tgtttttggc gggttgctcg gggtcaaagg aagaagtacc
     5161 tgataatccg ccaaatgaaa tttacgcgac tgcacaacaa aagctgcagg acggtaactg
     5221 gagacaggca ataacgcaac tggaagcgtt agataatcgc tatccgtttg gtccgtattc
     5281 gcagcaggtg cagctggatc tcatctacgc ctactataaa aacgccgatt tgccgttagc
     5341 gcaggctgcc atcgatcgtt ttattcgcct taacccgacc catccgaata tcgattatgt
     5401 catgtacatg cgtggcctga ccaatatggc gctggatgac agtgcgctgc aagggttctt
     5461 tggcgttgac cgtagcgatc gcgatcctca acatgcacga gctgcgttta gtgacttttc
     5521 caaactggtg cgcggctatc cgaacagtca gtacaccacc gatgccacca aacgtctggt
     5581 attcctgaaa gatcgtctgg cgaaatatga atactccgta gccgagtact atacagaacg
     5641 tggcgcatgg gttgccgtcg ttaaccgcgt agaaggcatg ttgcgcgact acccggatac
     5701 ccaggctacg cgtgatgcgc tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa
     5761 tgcgcaagct gaaaaagtag cgaaaatcat cgccgcaaac agcagcaata cataacagaa
     5821 acctgaaaca caaaacggca gcccttgagc tgccgttttt ttattctgtc agttgtgaaa
     5881 ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag atattcctca
     5941 agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaaa cttcgttgac aaaaagtgac
     6001 aaaattatga gattttcatc acacattttg acatcaggaa cggtatgctg aattcaccaa
     6061 gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac aaatggaaat
     6121 tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac
     6181 acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg ttgctgacgc
     6241 cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag atatgtacac
     6301 cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc agcacaaagg
     6361 cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga
     6421 agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac
     6481 agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac atgaaacaca
     6541 caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg
     6601 aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata
     6661 acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag agaaaatcag
     6721 cgcgctggat gaaaaattat tagcattact cgcagagcgg cgcgaactgg ccgtcgaggt
     6781 gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt
     6841 actggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg
     6901 cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc tccaacaaca
     6961 tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca aaggctccta
     7021 ttcacatctt gccgctcgtc agtacgctgc ccgtcacttt gagcaattca ttgaaagtgg
     7081 ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt
     7141 cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc tgctgcaaca
     7201 taccagcttg tcgattgtgg gcgaaatgac ccttaccatc gaccactgcc tgctggtttc
     7261 cggcaccaca gatttatcta ccatcaatac ggtctacagc catccgcagc cattccagca
     7321 atgcagcaaa ttccttaatc gttatccgca ctggaagatt gaatataccg aaagtacgtc
     7381 tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga
     7441 agctggcggc actttgtacg gtttgcaggt actggagcgg attgaagcga atcagcgaca
     7501 aaacttcacc cgatttgtgg tgttggcgcg taaagccatt aacgtgtctg atcaggttcc
     7561 ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt
     7621 gctggtactg cgcaaccaca atctgattat gacccgtctg gaatcacgcc cgattcacgg
     7681 taatccgtgg gaagagatgt tttatctgga tattcaggcc aatctgcaat cagcagaaat
     7741 gcaaaaggcg ttaaaagagt taggggaaat cacccgttca atgaaggtat tgggctgtta
     7801 cccaagcgag aacgtagtgc ctgttgatcc aacctgatga aaaagtgccg gatgatgtga
     7861 atcatccggc actggattat tactggcgat tgtcattcgc ctgacgcaat aacacgcggc
     7921 tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt
     7981 caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat
     8041 agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtca gcataaagct
     8101 gcggatcctg agcgaacagt cgcccgacca tcgccagctc aaggcggtaa atcggcgaag
     8161 agagcgccag aagttgctca agctgaacat tttcttctgc caggtgcagc ccgtaagcaa
     8221 aagtagcaaa gtggcgcagt gcctgaataa acgccatatt ctgatcgtgc tcgacggcgc
     8281 taatacgatg caaccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt
     8341 ccggtttacg tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccggac
     8401 cgaacattgg gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg
     8461 gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa ggcggtaatt
     8521 tgccaataac ttgctcagta acgtggattg gcacactaac aatcaccatt ccggcatcgg
     8581 caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg
     8641 agagtgtcag catcttctcg aacagacgtc ccatctgacc gccgccgcca acgataacta
     8701 ccgggcgtaa cgcaggacaa agcgttttaa atcctttgtc gttttcactg gagtaagatt
     8761 cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg
     8821 cctctgcacg acgcgaggcc aacatagatg cctctcgctc cggaacataa ataggcagtc
     8881 caaagcggct tttcacctct cccacttcag caaccagttc cagacgcttc gctaataaat
     8941 tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca
     9001 taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca
     9061 cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat
     9121 ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt actttcgatc
     9181 atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc
     9241 gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac
     9301 gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag
     9361 ttcggcgctt taccaccgcg caggatgaca tgcccgtccg gattcccctg agtttgtagc
     9421 aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc
     9481 atagcgttaa ttgccgttgc cagactgcca tcggtgccgt ttttaaaacc aaccggcatg
     9541 gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaatggct
     9601 gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc
     9661 agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct
     9721 tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg
     9781 gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg
     9841 gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa
     9901 ggaccacata ctaccagcag acgaggatcg cgcccggcga taatatctga aatggtttta
     9961 cgcgagtcag caatctgggc ttcttgttgc aggcttaatg gaaaagctgc cttcagttgt
    10021 tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttttgc
    10081 atgatggcga tcctgtttat gctcggttgc gatagttgat cctcagcgag gatgacgtaa
    10141 cgataacaca taaagtaaag ttttcaatcc atatttcgta catttttatt tacacatgca
    10201 atttagccgc gctttcaacc cttacctctg tatagataaa tttacacccc ctttaaaaac
    10261 aatccgctat gctttgaaaa aggagaaaga aatgatgaaa aagtttatcg cccccttgtt
    10321 ggctttactg gttagcggat gtcagattga tccttatact cacgcgccaa ccttgaccag
    10381 caccgactgg tatgatgttg gtatggaaga tgcgatatcg ggcagcgcca taaaagatga
    10441 cgatgcattt agcgattcac aggcggatcg cggtctatac cttaaaggat atgccgaagg
    10501 acaaaagaaa acttgccaga ccgattttac ttatgcccga ggactttccg gtaaaagctt
    10561 tccagccagc tgcaacaatg ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg
    10621 ggctgatgaa aatgccagca caatacgatt aaattaatta aaaccccaca aataaattaa
    10681 acataagatt ttactcattg tgttttattt ctcacattga tgacgatcgc atgaaaaaat
    10741 gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga tggtggggat
    10801 ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct gacgggagca
    10861 ccaatattcg ctcaggagcc ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt
    10921 attgtcagtt acacccgctg gcctgcgcta tcagggccac caaagttatg tatcttttca
    10981 tcttcacgtt ttagtaccgc cctacaagag aatgccgcga catctttgcc ttatctgcct
    11041 gtcatcattc acactcaaca agaggcgatg atttcaggct gcaatggttt ttattttggc
    11101 aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa ggcgttatta
    11161 ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct gatcatccac
    11221 aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc
    11281 aacccggatg tcttaatgct cgcacggaag aaaaaagatg gataacgata attctcttca
    11341 taagcgcccc acgtttaaaa gagcattacg caacatcagc atgaccagca tatttatcac
    11401 tatgacgctg atctggttgc tgctttccgt gacgtcagtg ctaaccctga aacagtacgc
    11461 gcaaaaaaac ctggcgctaa ccgctgcaac aatgacttac agcctggaag ctgcggtcgt
    11521 ttttgccgat ggtcccgcag cgacagaaac tctggccgca ctaggtcagc aaggccaatt
    11581 ttcaaccgct gaagtgcgtg ataatcagca aaacattatt gcctcctggc actacactgc
    11641 cagagaacca ggagatacct tcagtaattt cattagccgc tggctctttc cggcccccgt
    11701 cattcagccg attcgccaca atggtgaaac cattggcgaa gtgcagttaa ccgcacgcga
    11761 cagctcaatt agccatttta tctggttttc gctcgctgtc ctgaccgcat gcattctgct
    11821 ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggtctgg ttgaagcgtt
    11881 aaaaaacatc accgatgtcg tacatgatgt gcgttccaac cgcaattttt cccgacgagt
    11941 ttcggaagaa cgtatcgcag agtttcaccg cttcgctctc gacttcaaca gtctgctgga
    12001 tgaaatggaa gagtggcagc ttcgtttgca ggccaaaaat gcgcagcttc tacgaaccgc
    12061 gctacatgac ccattaaccg ggctggctaa ccgtgcagcg tttcgtagcg gcatcaacac
    12121 gttgatgaac agtgctgacg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
    12181 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct tgattgaaat
    12241 cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
    12301 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc agatatgctc
    12361 agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc agaccaccat
    12421 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa aattacaaga
    12481 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
    12541 acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca cctcactaat
    12601 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
    12661 atcttatgga tttactgaat ccgccggcga ctggtcgcta ggcttatcag atgccattct
    12721 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
    12781 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgataacta
    12841 tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc
    12901 atgggctatt ggtggacaaa ttccacgcag caatctcacc acacagggtt taggaaaaaa
    12961 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
    13021 ggtgattact accccttaaa acacgagcaa acagataaaa aaagggccag ccaattggcc
    13081 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
    13141 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
    13201 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
    13261 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
    13321 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
    13381 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
    13441 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
    13501 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
    13561 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
    13621 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
    13681 acagtaacac tggcggaacc tccatccctt ctaacacctc aggccgcgta tagtgcgggc
    13741 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtactc
    13801 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
    13861 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
    13921 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgctagc tcgctgacgc
    13981 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
    14041 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
    14101 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
    14161 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
    14221 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
    14281 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
    14341 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
    14401 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
    14461 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
    14521 ggtctttcca gtagtagtca ccctcttcaa gctgaggcag ttgcgatgaa tccacgacaa
    14581 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
    14641 tcatgtcctg attatggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
    14701 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
    14761 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
    14821 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
    14881 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
    14941 aatgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
    15001 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
    15061 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
    15121 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
    15181 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
    15241 tcgcataagg tcatttagcg accaggaaat cctggtggca tcatcccttt catgcttctc
    15301 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
    15361 tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc aatacggcgt
    15421 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat tgagttgatg
    15481 atggcttcca tacgcaccag cactttatcg tccatctgcg acttgacgtt atccgggatc
    15541 tgccccatgc ccggcagttt gcccatcaag ctggccatgc cgcccatatt tttcatctgg
    15601 cgcagttgct caagaaagtc gttgagatcg aagccgtcac cttttttcag cttgctggct
    15661 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
    15721 tcgcccatgc cgagaatacg cgacgcaatg cggtccggat ggaacggctc cagcgcctca
    15781 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
    15841 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
    15901 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
    15961 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
    16021 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
    16081 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
    16141 aaatcaacgc ccacctgctc agccagcgtc tcaagctgtt tgattgccgc cgggcgatag
    16201 acgtcggcag aaaccaccaa cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
    16261 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgctggc
    16321 ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
    16381 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
    16441 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
    16501 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
    16561 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
    16621 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
    16681 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
    16741 atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt ttttgctctg
    16801 ctcgcgcttg tcgcctattc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
    16861 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
    16921 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
    16981 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
    17041 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
    17101 gcgaccttca tgcccaatga atacatcacc catctggaag ctacgcctgg gatgctggtg
    17161 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
    17221 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa ccaggaaatg
    17281 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
    17341 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag catggaaaat
    17401 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
    17461 cattatcatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggtgcagtc
    17521 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
    17581 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
    17641 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
    17701 atcgtcttcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
    17761 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
    17821 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggtgatgca ggcgtggcaa
    17881 ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca
    17941 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag tattctgtta gcaccgctgc
    18001 aaattttgat gatgccgctg gtctggttgc tgaacgctat cacccgcatg ttaatgcgca
    18061 tgatgggcat caaaaccgat atcgtggtca gcggctcttt gagcaaagaa gagttgcgca
    18121 ctatcgtgca tgaatcgcgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
    18181 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
    18241 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
    18301 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
    18361 aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
    18421 cggacgagat ctattttgtc ccggaaggta cgcccctcag cacgcagttg gttaagtttc
    18481 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
    18541 tgacggttga agatattctg gaagagatag ttggtgattt caccacgtcg atgtcgccaa
    18601 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattattgat ggcactgcca
    18661 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
    18721 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
    18781 ttggcgagta cgatatcgat attctcgacg tacaggacaa tatgattaag caggtaaaag
    18841 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
    18901 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
    18961 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
    19021 acgttacctg gcgcaacgtc atcagattcc accattgcga tggcctgatg cacattcggg
    19081 tccagtggga cgttagtttc ggcgatcact tcaacgccaa acttacgcac aacatccagc
    19141 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
    19201 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
    19261 ttctccagcg cgaatttgtg ggctttttca atatccagtt cagtacgacg acgcaggttt
    19321 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccaac
    19381 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
    19441 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
    19501 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
    19561 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
    19621 catcaatctt cgcaacaagg acctcggaaa atgaataatc atttcaagtg tattggcatt
    19681 gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta ccgctggctg
    19741 tgcacaaaag gttacgaggt tatcgtcgag cagcaaatcg ctcacgaact gcaactgaag
    19801 aatgtgaaaa ccggcacact cgccgagatt gggcaactgg cagatctcgc agtagttgtt
    19861 ggtggcgacg gtaatatgct cggcgcagcg cgcacgctcg cccgctacga tattaaagtt
    19921 attggaatca accgtggcaa cctgggtttc ctgactgacc ttgatcccga taacgcccag
    19981 caacagttag ccgatgtgct ggaaggtcac tacatcagcg aaaaacgttt tttgctggaa
    20041 gcgcaagtct gccagcaaga ttgccagaaa cgcatcagca cggcgattaa cgaagtggta
    20101 cttcaccctg gcaaagtggc gcatatgatt gagtttgaag tgtatatcga cgagatcttt
    20161 gcgttttcgc agcgatctga tggcctgatt atttcgacgc caacaggctc caccgcctat
    20221 tccctctcag caggcggtcc aatactgacg ccttctctgg atgcgattac cctggtgccc
    20281 atgttcccgc atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt
    20341 ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcactg
    20401 ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt
    20461 catccgaaag attacagtta tttcaacaca ttaagtacca agctcggctg gtcaaaaaaa
    20521 ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata ctgtacacaa
    20581 taacagtaat ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa
    20641 ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg
    20701 cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg
    20761 cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc
    20821 tctgaaagat acgccagcgg ctctgcgctg gctggaagaa aaccagcttg aagacgggca
    20881 tgaatgtttg cttcgtcgcg tgatcagcag cgatggtcgc tcccgtggtt tcatcaacgg
    20941 tacagctgtt cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg
    21001 tcagcacgct catcagttac tcaccaaacc cgagcaccaa aagttcctgc ttgatggcta
    21061 tgccaatgaa acctctctac tccaggaaat gaccgcacgt tatcagttgt ggcatcaaag
    21121 ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgctgccc gtgcggaact
    21181 gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag agtttgagca
    21241 aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa
    21301 tgcattggca ttaatggccg acggtgaaga cgcaaacctg caaagtcagc tttacacggc
    21361 taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat
    21421 gctggaagaa gctaccatcc agattgctga agccagcgac gaactgcgcc actactgcga
    21481 tcgtctggat ctcgatccca accgactgtt tgaacttgaa cagcgcatct caaaacagat
    21541 ttcgctggca cgtaaacatc acgtcagccc tgagacattg ccacagtatt accagtcgct
    21601 actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc tcgcgctggc
    21661 ggtaacgaaa catcatcagc aggcactgga aacggcgcgc gcattacacc agcaacgcca
    21721 gcattatgca gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc
    21781 gcatgggcag tttacgattg atgtcaaatt tgacgagcat cacctgggcg ctgacggcgc
    21841 cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa
    21901 agtcgcatcc ggtggtgaat tgtcccgcat cgcattggca attcaggtca tcacggcgcg
    21961 taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac
    22021 agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt
    22081 tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac
    22141 cgatggtgcg atgacagaaa cgcatatgca atctctggat aaaaaagcgc ggttacaaga
    22201 gctggcgcgc ctgcttggtg gcagtgaagt cacacgtaac acactggcga atgcgaaaga
    22261 actgcttgcg gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt
    22321 aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca
    22381 gatgagccac gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc
    22441 tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag
    22501 tggtttaccg tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa
    22561 tacgtgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg
    22621 atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg
    22681 taacgcagca aacgctgacg ctaaccttta acagtagcgg tgtgttgacc aatattgata
    22741 acaaacctgc gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt
    22801 gctcaatgag cacctttttt ctgtccgtta tttattcgct gatttttctg ctcgttgcct
    22861 gcgaagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat caccatcatg
    22921 cacactatcg cttagttttg ccggacggct gtaaatgccg actttgtttt tagttaaatc
    22981 gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc
    23041 ctcctgcagc gtcacgcgct gcaggtactg cttctcaggt agcgcataag ccacctcaac
    23101 ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat
    23161 tagacgccag ctctttaaac acgcgaccaa aggcgagctc aatcaactta ttggtaaact
    23221 caaagtcgag atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc
    23281 caccaatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg
    23341 tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg
    23401 cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact
    23461 gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca
    23521 gtgcggtccg gctaatctga ggcataacaa tttccatcaa caaaaatcca acaaataata
    23581 tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct
    23641 aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga
    23701 tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg
    23761 cttaacaagc gcgcccgtca cgaatacttt atcgaagaag agttcgaagc gggacttgcc
    23821 ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag ccaatatcag cgacagctac
    23881 gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggccgta
    23941 gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc
    24001 gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc
    24061 ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taagaaacag
    24121 cacgataaac gttcagatat caaagagcgc gaatggcagg tggataaagc gcgtatcatg
    24181 aaaaacgccc accgttaaac ctgcactcca attattgacc agttcctcac cgcgcctccc
    24241 tctccggcgg cgcgaatgaa catcttattg gttatcacat ccgacacaaa tgttgccatt
    24301 ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc
    24361 attgaggctg gtcatggcgc tcataaatct ggtatactta cctttacaca ttggggctga
    24421 ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt
    24481 aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct
    24541 gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc
    24601 caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag
    24661 tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt
    24721 agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccacttta
    24781 agaaggacta caaccggaca gtagcaataa atacagccac ttacggacac aagccagaca
    24841 gtagtcggac tgaaaaaaga caaaaatata cacgtgaaat gcacgtgcac ttctaaaaaa
    24901 ccccagttat caccgactgg ggtttttcaa tttgtaacta agggtaacaa aaacaccccc
    24961 gtttttttca tctcactcac cttgacactg tttatttgta cagttaaaaa taacactgta
    25021 tacaaacaca gtataggggg acttttatgc gtattgaaat ctgcatagcc aaagaaaaaa
    25081 tgac
//
LOCUS       DAZUGK010000049        24793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZUGK010000049 DAZUGK010000000
VERSION     DAZUGK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.049"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..565
                     /locus_tag="ACHY5W_001783"
     CDS             <1..565
                     /locus_tag="ACHY5W_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFS4446692.1"
                     /translation="LSGCGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVACIKGY
                     SNHEKDVLEAVTLARSQANRASSDLQQAPGDAQKLQAWQQAQAQVTRTLGQLTIISER
                     YPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTP
                     KKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            579..1250
                     /locus_tag="ACHY5W_001784"
                     /pseudo
     CDS             579..1250
                     /locus_tag="ACHY5W_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1487..2635
                     /locus_tag="ACHY5W_001785"
     CDS             1487..2635
                     /locus_tag="ACHY5W_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4446693.1"
                     /translation="MRIFLLLLMLCGFQVFAEQWPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSIVCFSLLLATTCKQRLAVVFNLCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2632..3534
                     /locus_tag="ACHY5W_001786"
     CDS             2632..3534
                     /locus_tag="ACHY5W_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFS4446694.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     REDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELSEDKGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     RGSSGGGVSGGGGSSGGGGASGRW"
     gene            complement(3594..4892)
                     /gene="eno"
                     /locus_tag="ACHY5W_001787"
     CDS             complement(3594..4892)
                     /gene="eno"
                     /locus_tag="ACHY5W_001787"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4446695.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4980..6617)
                     /gene="pyrG"
                     /locus_tag="ACHY5W_001788"
     CDS             complement(4980..6617)
                     /gene="pyrG"
                     /locus_tag="ACHY5W_001788"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFS4446696.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6845..7636)
                     /gene="mazG"
                     /locus_tag="ACHY5W_001789"
     CDS             complement(6845..7636)
                     /gene="mazG"
                     /locus_tag="ACHY5W_001789"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFS4446697.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7707..8042)
                     /gene="mazF"
                     /locus_tag="ACHY5W_001790"
     CDS             complement(7707..8042)
                     /gene="mazF"
                     /locus_tag="ACHY5W_001790"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFS4446698.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8042..8290)
                     /gene="mazE"
                     /locus_tag="ACHY5W_001791"
     CDS             complement(8042..8290)
                     /gene="mazE"
                     /locus_tag="ACHY5W_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFS4446699.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8368..10602)
                     /gene="relA"
                     /locus_tag="ACHY5W_001792"
     CDS             complement(8368..10602)
                     /gene="relA"
                     /locus_tag="ACHY5W_001792"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFS4446700.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10650..11951)
                     /gene="rlmD"
                     /locus_tag="ACHY5W_001793"
     CDS             complement(10650..11951)
                     /gene="rlmD"
                     /locus_tag="ACHY5W_001793"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4446701.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12008..14764
                     /gene="barA"
                     /locus_tag="ACHY5W_001794"
     CDS             12008..14764
                     /gene="barA"
                     /locus_tag="ACHY5W_001794"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFS4446702.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14996..16336)
                     /gene="gudD"
                     /locus_tag="ACHY5W_001795"
     CDS             complement(14996..16336)
                     /gene="gudD"
                     /locus_tag="ACHY5W_001795"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFS4446703.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16357..17697)
                     /gene="gudX"
                     /locus_tag="ACHY5W_001796"
     CDS             complement(16357..17697)
                     /gene="gudX"
                     /locus_tag="ACHY5W_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFS4446704.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGELIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17699..19051)
                     /gene="gudP"
                     /locus_tag="ACHY5W_001797"
     CDS             complement(17699..19051)
                     /gene="gudP"
                     /locus_tag="ACHY5W_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFS4446705.1"
                     /translation="MSSLSQAASRVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19486..19935)
                     /gene="yqcA"
                     /locus_tag="ACHY5W_001798"
     CDS             complement(19486..19935)
                     /gene="yqcA"
                     /locus_tag="ACHY5W_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4446706.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTTQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19953..20735)
                     /gene="truC"
                     /locus_tag="ACHY5W_001799"
     CDS             complement(19953..20735)
                     /gene="truC"
                     /locus_tag="ACHY5W_001799"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFS4446707.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20735..21064)
                     /gene="yqcC"
                     /locus_tag="ACHY5W_001800"
     CDS             complement(20735..21064)
                     /gene="yqcC"
                     /locus_tag="ACHY5W_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFS4446708.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21686..22231)
                     /gene="syd"
                     /locus_tag="ACHY5W_001801"
     CDS             complement(21686..22231)
                     /gene="syd"
                     /locus_tag="ACHY5W_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFS4446709.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22299..23147
                     /gene="queF"
                     /locus_tag="ACHY5W_001802"
     CDS             22299..23147
                     /gene="queF"
                     /locus_tag="ACHY5W_001802"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFS4446710.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGADIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23259..24623
                     /gene="ppnN"
                     /locus_tag="ACHY5W_001803"
     CDS             23259..24623
                     /gene="ppnN"
                     /locus_tag="ACHY5W_001803"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFS4446711.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6093 a         6508 c         5952 g         6240 t
ORIGIN      
        1 tttatcaggc tgtggatata acgatattca aacctatgat gagcaggtta acgcctcctg
       61 gtctgaggta ttaaatcaat atcaacgtcg cgccgatctt attcctaatc ttgtcgcctg
      121 tattaaaggt tattccaacc atgaaaaaga cgttttggaa gccgtaacat tggctcgtag
      181 ccaggctaat cgcgccagca gcgatcttca gcaagctccc ggagatgcac aaaaattaca
      241 agcctggcaa caggcccagg cgcaggtgac tcgcactctc ggtcagttaa cgataattag
      301 tgagcgttac cctgagttaa aggctcaaga gctctatcaa aacttaatgg tacagctcga
      361 aggaagtgaa aatagaattg ccgttgcgcg aggaagatat attaaagcca ttgaacaata
      421 taatgtcact atccgcaaat tccccgctgt attaaccgca aaggtcatgg attacacgcc
      481 aaagaaaaat tatttaccgg atgatgtcgc cgcagtaagt aaagccccaa caatagattt
      541 tagccagaat gctaatgctc attaagcggg gaagttctgt gcgaaatttt atcttcctta
      601 tggctttctt ctgttcatct gtgtttgcca cacaaattcc agtacctgaa tcccccaagt
      661 atgtgaatga cttaaccggc acattaacaa acagcgaagt aaacaccctg accaaccaga
      721 ttaaagccct gacgcaaaaa agccacgcac aactggtcgt gttagtggtt gaaacaacag
      781 gcgatgaaac catcgagcag tacgcaacgc gggtattcga tagctggaaa ccgggtgata
      841 aagaccgcga tgatggcgtt ctactgttag tcgcctggca ggatcatacc gtgcgtattg
      901 aaatcggtta cggactggaa ggtattatta cggatgcgca gtccgggcaa attattcgca
      961 acagtattat tccggcattt aaaaatggtg atcttgctgg cggtttacaa aaggggatca
     1021 gcgatattga aagtcgtctt acggggaata atttagcaac gataacaccg accgatcacc
     1081 ctttgccctt tagcggttgg tgggcattgt tagtctgggc gatagtactg acgtttattt
     1141 ctgccagagg atatatcaaa acgctgggag taatttgctt cgcagctatt gtactggcct
     1201 ttgttttacc tatagcaggc ttcagcggaa gctggggagt actcgcaaca ttactctgct
     1261 tcgccacacc gttcctggct gtcgccatcg cttttacccc cttcggcaaa aaagttcggg
     1321 atttgatgcg tgatgtcaac cagccatcac gccatacaag aagcaattct tcctcccact
     1381 attcatcatc gtcatcctct tcattcaaca atgacaattt cagcggtggt ggcggttcat
     1441 ctggtggcgg tggcgcttcc gggcgctggt aacaaaagga attgatatgc gtatttttct
     1501 cttacttctg atgctatgcg gttttcaggt attcgctgaa caatggcctt cagtaactga
     1561 cttaaccggg acattaacga cagaagaaca aactgcttta actcaacaat tacagactct
     1621 cgagcagcaa aaccacttcc aggtggccgt gttggttacg ccgaccacag ggggcaaaaa
     1681 cattaaactt gcagcctcgc agagatacgg ggtatatcac tggaaacccg ttggagagaa
     1741 gcgatatggc gaaggtatct taattctcgt cgtatggccg gaagggctgg cgtcaatgaa
     1801 gattggacat ggactggaac agatgttgcc gccggaacaa gctgcgcaaa ttgttcgtta
     1861 ccatatgcag ccagaattcg aaaaaaacaa tctctttgct ggattaacgg gcggaattga
     1921 aagcatcgca cagtttaccc atatcaaagc gaaccttagc cctttggatg ctttagccaa
     1981 tcatctgttt gctaatccgc aacgttcgct gccatgtctt gcctggacac tgctgatgat
     2041 cgttgccatc gttgtcctct ggcgatttgc cagccgccca cggcgtggca tatggatgat
     2101 atcgatgatt acccctatgg tctggttttt ctcctttcag gacgatgtca tcattcggcg
     2161 ggtgtcgatc gtttgtttta gtttattgtt agcaacaacc tgcaagcagc gtctcgcggt
     2221 agtttttaat ttgtgccgcg tatttcctat gatgctggcc ccaaaaaata aaaacgctaa
     2281 agtgaaaaaa caaaagaaga ctccgcaggt tcgtgaatca ggacgaaatt cattgtttgt
     2341 cgtgatgctg ataattattg tgggatggtg cctggtgttc gccagtaata aagatattgc
     2401 attcatctca acaattattg ggttaattga tcattttatc ggcccaataa ccattgctgg
     2461 catcgttctg ctgatcattg tggccaaatt tactggcaat cttaagttgg gatcaggaga
     2521 aaaaagtaac aggaaaacgg ggaatcgtaa tgctcactct cgctcttcgt cgagaaattc
     2581 tttccgcggt ggtggcggtt cttcaggagg cggcggttca tccggccgtt ggtgatcgta
     2641 atgcgcttaa tctttattct acttgctttg tggagccttc ccgggcttgc ccagcaaatt
     2701 gctgtgccgg agctgcgcca acaggtgacc gatattactg gtacgttaag cacttctgaa
     2761 caacaatcac tgacacagca actgcaggat attacacata aaacacgggc gcaggtcgct
     2821 gtattagtag tgccgtccac cggagacgat tccattgaac aatatgctac aagggttttt
     2881 gatagctgga aactgggaga taagcagaga aacgatggta ttttacttct ggttgccagg
     2941 gaggatcatg ctgttcgtat tgaggttggc tacgggctgg aaggggtggt taccgacctg
     3001 caagcggcga aaattatcag ggatatattg atccctgcct tcaaaagtga tgacctgatg
     3061 ggtggattaa cactggcaag cgaaaatatc ggcgcgcttc tgttgaatgg tgaattatcg
     3121 gaagacaagg gggattatta cagtatcaat cctcctattc cattatcgct tgctgtaatt
     3181 atattgctgg cggtactttc atattttatt gtttttaccg atccgtcgaa tttaccctgg
     3241 atcactctca ctggcgccat ttacgggatg gtattcctct atgttgccga gccaggacca
     3301 tggaccaatt taatcgttgc ctgcggtatg ttaacgcctt ttgcgatcgt accactggtt
     3361 attttttggc ttatcgtcaa taaaaagcta cgcgccaaat acaaaaaact cagtaaagaa
     3421 agagcttcaa gaaaaggttc ttcttcatcc tcttccggta gaggctcaag tggcggcggg
     3481 gttagcggcg ggggcggttc ttctggcggt ggcggcgcgt ccggtcgctg gtaactcaac
     3541 cataaaaaac gccagcccga aggctggcat ttttaaatca gataaagtca gacttatgcc
     3601 tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc ttcttcgata
     3661 cgaatcagct ggttgtattt agcaacgcgg tcagaacggc tcatagaacc agttttgatc
     3721 tggcctgcag cagtaccaac agccaggtca gcgatggtag cgtcttcagt ttcgccagaa
     3781 cggtgagaga tcactgcagt gtagccagcg tctttcgcca tcttgattgc agccagagtt
     3841 tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat acctttttcg
     3901 ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac cagctggatt
     3961 ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc gtccagaccg
     4021 tcttcgatgg aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg agtgaattct
     4081 tcagaggtga atgctttgtt gccttcgcca gccagaacgt atttaccgtc tttgtagaat
     4141 tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata accagcagct
     4201 ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt cggcgcatag
     4261 ccaccttcgt caccaacagc agtgttcatg cctttcgctt tcagaacttt tgccaggtga
     4321 tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc aaccggctga
     4381 atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt gatgatgttc
     4441 atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc gatgtgctcg
     4501 tacagcggca tccctttagc ggcagcagca gctttggcgt tagccagaga tacagccagg
     4561 attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat catgatcttg
     4621 tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat cgggccgttt
     4681 accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc gccatcgcgc
     4741 agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc cataccgacg
     4801 aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga gtcgatgatt
     4861 tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca ctagttaaac
     4921 tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa cttttttact
     4981 tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc aaacagcggg
     5041 tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac gaaccacggg
     5101 tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc
     5161 agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg gtgacgatga
     5221 cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc aaccaactgg
     5281 cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct acgaacttca
     5341 acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta cttacagtct
     5401 ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa catggcgagc gtaatcaatt
     5461 aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc acgcgcaaaa
     5521 cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg tacgaggatt
     5581 gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt
     5641 ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc gatcacggat
     5701 ttataagcat ccggcagttc aatgtacttg ccgaccatac cgatggtgac ttcacttacc
     5761 ggattcgctt cttcgaagat aacctgttcc cattcggaca gattcgcttc tgggcagttt
     5821 aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag gcccggaatt
     5881 ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac attacagaac
     5941 aatgcaatct tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca aatcaggatg
     6001 tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg tttggttttg
     6061 acttcaccag acgccgccat gtacggcacc agcgtcaggt gcataaacag ggtgtgctca
     6121 cggccaattt caacagccat ctggcgaatc gcttcgagga acggcaggga ttcgatatca
     6181 cctacagtac cgccgatttc gaccagtact acgtcatgac cttcgccgcc ttccagcaca
     6241 cgctctttga tagcgtttgt gatgtgcgga ataacctgta cggttgcgcc gaggtagtcg
     6301 ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg
     6361 cggctcattt tggtacgaat gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg
     6421 ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc tggatcgacg
     6481 ttgatgtacg gatccagttt catgatggtc acattgaggc cacgggcttc aagaatggct
     6541 gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc ggtcacaaaa
     6601 atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat ggaataacca
     6661 ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt atccctcctc
     6721 ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg taaatgtttt
     6781 tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atggcgcttg accgcgtaat
     6841 tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc attgtttcga
     6901 ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact tcgcgaaaac
     6961 gacgctcgaa tttttcgttc gctttttgca atgcgatttc tgctttcgtc cctaaatggc
     7021 gagccagatt aaccgtggca aacagcaggt cgcccatttc ctcctccagt ttagcctggt
     7081 cgataacagc ctgccgtgcc tcgtacatca cctcgtcgat ctcttcgtag actttatcaa
     7141 ccactgggcc aagcgtcgtc cagtcgaagc caacgttggc gcagcgtttc tggatttttt
     7201 gcgcccgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa tgctgcgctt
     7261 tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca ctactgtttt
     7321 cggcagaact gtcagcaaaa acatgcggat ggcgacgctc taatttatcg ctaatagcag
     7381 cgcaaatatc attaaagtca aagcgccctt cttcctgagc catttgcgcg taaaacacca
     7441 cctggaatag cagatcgccc agttcaccgc gcagatcgtc aaaatcttca cgggcgatgg
     7501 cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca aatgtctgct
     7561 ctttatccca cgggcagccg ttttccgggt cgcgcaggcg ctgcataata gtgagcaaac
     7621 ggtcgatttg attcattaga atgtcctgaa aattgcgggt ccgtcaggtg gaaacctgtg
     7681 accagaatag aagtgagtta gtaacactac ccaatcagta cgttaatttt ggctttaatg
     7741 agttgtaatt cctctggggc aactgttcct ttcttcgttg ctcctcttgc ccgccaggcg
     7801 atacttttta cctgatcagc taacgctacg ccatcacgtt cctgaccgga taaaacaact
     7861 tcgaacggat atccttttga ttgcgttgta caaggaacat acagacacat acctgttttg
     7921 ttgttgtaca tgaacggact caggacaaca gccggacgat gtccggcttg ctcgctacct
     7981 tttgtcgggt caaaatcaac ccaaatcaga tcgcccatat cgggtacgta tcggcttacc
     8041 attaccagac ttccttatct ttcggctctc cccagtcgat attctcgtgg aggttttccg
     8101 gcgtgatgtc gttgaccagt tcagcaagcg taaatacggg ctctttacgc actggctcaa
     8161 taattaattt gccatccacc aggtcaatct tcacttcatc atcaatattc agattgagcg
     8221 cctgcattaa cgtagccggg atccgcaccg ccggtgaatt tccccaacgc tttacgctac
     8281 tgtggatcat aaccctttcc tcaaaccgct atcatatgta gatacagtat atatcaatct
     8341 acattgtaga tacgagcaag tttcggccta actcccgtgc aaccgacgcg cgtcgataac
     8401 atccggcacc tggttgagtt tacccagcac gcgacccagc acctgcaggt tgtaaatctc
     8461 aatggtcatg tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg caacgccaag
     8521 cacgttcact ttctcattgg cgagaatggt cgtgatatca cgtaacaacc cactacgatc
     8581 attagccacc acgcggacca ccagcgaata tccggcggag tagctctcgc cccataccgc
     8641 gtcaacaatg cgttctggcg catgggagcg cagttccgcc agttgttcgc aatcggcgcg
     8701 gtgtactgaa ataccgcgtc cttgggtaat gaagccgaca atctcatctc caggaatcgg
     8761 ctggcagcag cgcgcgatgt ggtgcatcag gttaccgaca ccttcaacca ccacacgacc
     8821 gttatcttta ctgcggtttt gcggcgtgta gcttttttgc tgaagttgct tcagcgcggc
     8881 ggcgtcctgc tcttcggcac tcggcttatt aaattgcgat tgcaggaagt tcaccatctg
     8941 attgagacgg atatccccgc caccaatcgc cgccagcaac tcgtcgacat cattgaagtt
     9001 gtaacgcggc agcagatgtt tttctgcttc tttcaggctg atccccagat gttccagctc
     9061 gtcgtcaagg atttgccgcc cagccagaat gtttttgtca cggtcctgtt tacggaacca
     9121 ggcgtgaatt ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca
     9181 gtcacggctg gggttcggct gtttctgggt gataatttca atctggtcgc ccatctgcag
     9241 ctggtaggtg aacggcacaa tgcgcccgcc aatttttgcc ccgatgcagc ggtgtccgac
     9301 atcactgtgg atgtggtaag cgaagtccag cggcgttgat cccgcaggca aatcaacgac
     9361 atcacctttc ggcgtaaaga catacacccg gtcgtcaaac acctgactac gtacttcgtc
     9421 gagcatttcg ccggaatcag ccatctcttc ctgccacgca atcagtttac gcagccaggc
     9481 aatccggtct tcatgtcccg aacgtgcgcc gccagcagcc gcgccctctt tatatttcca
     9541 gtgcgcagca acacccaact ctgcatcttc atgcatctgt ttggtgcgga tttggatctc
     9601 aacggttttt ccacccggcc ccagaaccac ggtatgaata gactgataac cgtttggttt
     9661 cgggttagcg acgtaatcgt caaactcatc cggcaggtgg cgatagtgag tgtgcactat
     9721 ccccagtgcg gcataacaat cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc
     9781 aaacagctca tcgaaggcga ggttcttttt ctgcattttg cgccagatgc tgtagatgtg
     9841 tttcggacga ccatacactt cagctttaac gccttcagct ttcatctcag cgcgcagatg
     9901 gccgacaaac tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca acagttttgc
     9961 gatgcgtttg tattcggtcg ggtggagata acggaagcag taatcttcca gctcccattt
    10021 cagttgccca atcccaagac ggtttgccaa cggcgcgtag atattggtgc actcttttgc
    10081 cgccagtacg cgttcatctt ccggcgcatc ttttacttca cgcagatgag caatacgctc
    10141 cgccagtttg atgaccacgc agcgaaaatc atcgaccatc gccaataaca tccggcgaac
    10201 gttatcgacc tgttcggagg aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc
    10261 cgccatatca cgcacgccgt gaataaggtt aacgaccgac ttaccgacgc tctcacgcag
    10321 cacatcttcg ctgactacgt tggcatcagc cagagggaac agcagcgccg cccgcagcgt
    10381 gtcaatgtcc atacttaatg tcgagaggat ctccaccatc tcaacaccac gccacaataa
    10441 cagactggca tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa
    10501 gcactcacac gacttctggc tggtaatacc cagacttgcg atccattttt ccggatcaaa
    10561 ttcaccagcc ttattgatat gtgcacttct taccgcaacc atcgtcctct cctttaggga
    10621 ccagacctgc cgaaatcggc aaatcgcaac tatttaacgc gcgagaaaag taccatcgat
    10681 tccagatgtc ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct
    10741 gcttttaata acgcttcgct atcccgagcc agcgttgcag ggttacagga tacataaact
    10801 atacgaatag gttccagttt tataatttgc tgcataacac ctgcggcacc tgctcgcgcc
    10861 gggtccagca acactttatc gaagccgttt ttcgcccacg gctgctttgt gacatcttct
    10921 tcaagatttt catgataaaa cgtcacattc tgtaagccat taagacgcgc attctgctgg
    10981 cctttttcca ccagcgccgg aacaccttct acacccacca cactggctgc ttgtgtcgcc
    11041 aatggcagtg taaagttgcc cataccgcag aacagatcca gtacgcgatc ttcaggttgc
    11101 acatccagcc attccaacgc acgcgctacc attttttggt tcacacccgc attgacctga
    11161 ataaaatcgc gcgggctaaa agttaagcgc aacccgtttg agtcatacca gggcatctca
    11221 ccagagacgg tttcgagtat ctcactatcg ggggcgagat acagatccag gccttcagaa
    11281 tgcgaaaagc gttccagttt ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa
    11341 atcatcagcg tgccgctggt tgcctgtacc agttcaacat gaccaagatg gcgcatagct
    11401 tgtaagctgc ccaggcatgc cctgactttg ggcagcaatg cttcaagttg gggcactaaa
    11461 atggggcatt gtttaacgtc gacaatgtca ctggagcccg ctttgcgaaa ccccatctga
    11521 agttgctgtg ttttcggtaa gtagttcaga cttaaacgcg cgcgacggcg atagccccag
    11581 ggaacatcgg cgatcacttc agagacttcg tgtttcatta atcgggcgag tgccgcactt
    11641 ttgcttcgct gctgtaaatc cacgctggcg tgttgttgct gacagccgcc gcatacgcca
    11701 aaatgaggac agcgtggcgt ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta
    11761 gcgcgggcat actgtttttt atcttcagta acagtaactt ccgcgttttc ctgcggcaat
    11821 aatccgggga taaatagcgt tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg
    11881 aggtcgttga ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag
    11941 aattgcgcca ttggcgagac tttctcaatt taacagtgtg accttaattg tcccataacg
    12001 gaactccatg accaactaca gcctgcgcgc acgcatgatg attctgatcc tggcaccgac
    12061 cgtccttatt ggtttattgc tgagtatctt tttcgtcgtg catcgctata acgacttgca
    12121 gcgtcaactg gaagatgccg gtgccagcat tattgagccg cttgcagttt ctactgaata
    12181 tggcatgagt ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg
    12241 ccattccgat attgttcgcg cgatttcggt ttatgatgaa aataaccgac tctttgtcac
    12301 ctccaatttt catcttgatc cctcatcgat gcagctcggc agcaacgtgc cgtttcctcg
    12361 ccagctcact gtcactcgtg acggcgatat tatgatcctc cgcacgccga ttatttctga
    12421 gagttactcc cccgacgaat cgcccagtag cgatgccaaa aatagtcaaa atatgttggg
    12481 atatattgcg ctggagctgg atcttaaatc ggttcgcttg cagcaatata aagagatctt
    12541 tatttccagc gtgatgatgc tgttttgtat cggtattgcg cttatttttg gctggcgctt
    12601 aatgcgcgat gtaaccggtc cgattcgcaa catggtgaat accgtcgacc gcatccgtcg
    12661 cgggcaactc gacagccggg tggaaggatt tatgctcggc gagctggata tgctgaaaaa
    12721 cggtatcaac tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc agcacaatat
    12781 cgaccaggcg acgtccgatc tgcgtgaaac gctggagcag atggaaattc agaacgttga
    12841 gttggatctg gcgaaaaagc gcgcccagga agcggcgcgt attaaatccg agtttctggc
    12901 aaatatgtca cacgagctgc gtacaccact gaatggtgtt attggcttta cccgcctgac
    12961 gctgaaaaca gaattaacac caacgcagcg cgatcacctg aatacgattg aacgttcggc
    13021 aaataatttg ctggcaatta ttaatgatgt tctcgacttc tcgaaactgg aagcaggtaa
    13081 gctgattctg gaaagtattc cattcccact acgcagcacg ctggatgaag tcgttactct
    13141 gctggcacat tcttctcacg ataaagggtt ggaactgacg ctcaatatta aaagcgacgt
    13201 gcctgataac gtgatcggcg acccactgcg attacagcaa atcatcacta acctggtggg
    13261 gaatgcaatt aaattcaccg agaatggcaa cattgatatt ctggtagaaa aacgtgcgct
    13321 gagtaatacc aaagtgcaga ttgaagtgca gattcgggat accggcattg gtattcctga
    13381 acgcgatcaa tcgcgcttat tccaggcctt ccgacaggct gatgccagta tttcccgccg
    13441 tcatggtggc accgggctgg ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg
    13501 cgatatttcg ttccatagcc agccgaatcg cggttcaact ttctggttcc acattaatct
    13561 cgatctgaac ccgaacatta ttattgaagg gccatccacc cagtgcctcg ccggtaagcg
    13621 cctggcctat gtcgaaccaa actccgcagc agcgcaatgc acgctggata ttttaagtga
    13681 aacgccgctg gaagtggttt atagcccaac gttctccgcg ctgcctcccg cgcattacga
    13741 catgatgttg ttaggcatcg cggtgacctt ccgcgagccg ctaacaatgc aacatgagcg
    13801 attagcgaaa gcggtatcga tgaccgattt cctgatgctg gcacttcctt gccatgcaca
    13861 agtcaatgct gaaaaactta agcaagatgg tatcggcgcg tgtctgctga aaccattaac
    13921 acctacgcgc ctgttgcctg ccctgacgga attttgtcat cacaaacaaa acacgctttt
    13981 gcctgtaacc gatgaaagta agctggcaat gacagtcatg gcggttgatg acaaccccgc
    14041 taacctgaaa cttatcggcg cattgctgga agatatggtg caacatgtgg aactttgcga
    14101 tagcgggcat caggcggttg aacgggcgaa acagatgccg ttcgatttga tcttaatgga
    14161 tattcaaatg ccggacatgg acggcattcg ggcctgcgaa ctcatccacc agctccccca
    14221 tcagcaacaa acgccggtta tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa
    14281 gctgcttggc gcagggatga gtgattatct ggcgaaaccg attgaagaag agcgattgca
    14341 taatttgctg ttgcgctaca agcctggcag cggtatttcc tctcgcgtcg tgacgcccga
    14401 agtcaacgaa attgtggtga acccgaatgc gaccctcgac tggcaactgg cactacgcca
    14461 ggcagcagga aaaaccgatc tggcgcgcga tatgctgcaa atgttactcg atttcctgcc
    14521 tgaagttcgc aacaaagttg aggaacagct ggttggagaa aacccggaag gcctggttga
    14581 tttgattcat aaactgcatg gcagttgcgg ctatagcggt gtgccgcgta tgaagaatct
    14641 ctgccaactg atcgaacaac agttacgtag cggtactaaa gaagaagatt tggagccgga
    14701 gctgctggaa ctgttggacg agatggataa tgtcgcgcgc gaagccagca aaattctcgg
    14761 gtaatggacg tcttgtcgag tagcaatcgg ctttttatga agctggtttc agattggtga
    14821 aaaagtgcct gtgtttatgc cggatgcggc gttaacgcct tatccggcct acaaaaccaa
    14881 ttaaattcaa tgaattaaaa aatgatgtag acctgataca tagcacaaca ggctattttg
    14941 cgtttagcat cagtctcaaa ccggctccag atagagccgg ttttggtttt ctgtcttaac
    15001 gcaccatgca cgggcgcttg ttatcgaacg tccagccagg aatcagatac tgcattccca
    15061 tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag ctcatgggct ttcatcactt
    15121 gatccatatc gatttctaca cccagccccg gtttttctgg cacctgtacc agcccgcctt
    15181 tgatctcaaa cggttctttg gtcaggcgct gattaccttc ctgccagatc cagtgcgtat
    15241 caatagcagt aattttaccc ggtgcagcgg cggcaacatg ggtaaacatc gccagggaaa
    15301 tatcgaagtg gttgttagag tgtgaacccc aggtcaggcc aaattcatgg cacatttgcg
    15361 ccacacgtac cgaaccttgc attgtccaga aatgcggatc cgccagcggg atatcaacgg
    15421 attgtaacga gagcgtatgg cccatctgcc gccagtcggt ggcgatcata ttggttgcgg
    15481 ttggtagacc ggtcgcgcga cggaactctg ccatcacttc acgcccggag aaaccttgct
    15541 ccgcaccaca cggatcttct gcataagcca gcgaaccttt caggtatttg ccgattttaa
    15601 tcgcttcgtt cagcgaccag gcaccgttag gatcgagcgt aatacgcgcc tgcgggaagc
    15661 gttgcgccag tgccacaata gactcggcct cttcttcccc ggccagtaca ccgcctttca
    15721 gtttgaaatc gttgaagcca tatttttcat acgccgcttc cgccaggcgc accaccgcat
    15781 ccggcgtcat cgcttcttca tgacgcaggc gataccagtc gcatgagtca tccggctggc
    15841 tttgatacgg cagaggtgtg gctttgcgat taccgacgaa gaacagataa ccgagcattt
    15901 cgacttcgct acgctgttga ccatcgccca gcagcgatgc cacgtttacc cccagatgct
    15961 gccccagcag atccagcatt gccgcttcta tcccggtaac tacatgaata gtggtacgca
    16021 aatcaaatgt ctgcaaaccg cgcccaccag catcacgatc ggcaaaagta ttacgcacca
    16081 gcgtcagaac gtttttgtat tcacccagcg ttttacctac caccagcgga atcgcatctt
    16141 ccagcgtttt acggattttc tcgccgccgg gaatttcccc tacgccagtg tgaccagaat
    16201 tatctttgat aatcacaata ttacgcgtaa agaacggtgc gtgtgcacca ctcagattca
    16261 tcagcatact gtcatgaccc gccaccggga tgacctgcat ttcagtaaca acaggcgtcg
    16321 taaattgaga actcatattt atgtcctttt tcagaatcaa tgacggccga aaacgggacg
    16381 tttacggtca aaggtccagc cggggatcag gtactgcatc gggcctgcgt cgttacgcgc
    16441 accgccaggc agacgtttat aggcctcatg tgccttttgt acctgttccc agtccagttc
    16501 cacgcccaga ccgggcgcat caggaacggc aatttttccg tttttaatct ccagcggatt
    16561 ttgggtcagg cgacaatcgc cctcctgcca aatccagtgg gtatcgatag cggtaggatt
    16621 acccggcgcc gccgcgccca catgggtaaa catcgccaga gagatatcga aatggttatt
    16681 agaatggcag ccccaggtca gcccccagtc gtcgcaaagc tgcgccacac ggactgcacc
    16741 ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct accgcattga gcatcaccgc
    16801 atgacccatt tcgcgccagt tggtggcgat catgttagtc gcgacgggca agccggtcgc
    16861 ccgccgaaac tccgccatca cttcacgtcc ggagaagccc tgttctgcgc cgcatggatc
    16921 ttcggcatag gtaagaacat cattcagccc tttgcacaga gaaatggctt catcaagcag
    16981 ccatgcaccg ttgggatcaa cggtaatccg cgcatccggg aagcgtttct tcaatgcacg
    17041 aacagtgtcg atttcttgct cgccaggtaa cacgccgccc ttaagtttga aatctttaaa
    17101 gccatagcga tcctgtgagg cttccgccag acgcacaacg gcttcgctgt tcatcgcttt
    17161 ctgatggcgc aactgatacc actcatggtt gcccggcgta ttttccagat aaggaagatc
    17221 ggttttggtc cgatcaccga tataaaacag ataaccgagg acggtaatag cctcgcgttg
    17281 cttgcctggc cctaacagtt cgcagaccgg aacattcagc gccttaccta gcaggtcaag
    17341 caaggcggct tccagcgccg ccacggcgtt aacgcgcaat tcaaaggtcc aggccccttt
    17401 accgaaggtt tcaaaatcgg cggcctgatt acctttatgc acctgctgaa ccactttatt
    17461 caggcgcgca acttcctggc ccagcaccat cggaatagct tcgaccagtg tttgataaat
    17521 caactctccg cccggcgctt caccaatgcc ggtatgcccg gcgttatcgg tgagtaccac
    17581 gatattgcgg gtgaagtatg cgttatgcgc accaccaata ttgagcaaca tgctgtcatg
    17641 tccggcgacc ggaatgactt tcatatcagt aataacagga ctggattgtg tcgtcatctt
    17701 attgccccgc gacaggtttc agctcgatac gcttgatatc gcccaccagc accaggtagc
    17761 tcagtaccgc gattaaggca tgaacaccaa cataaatcag cgccccatta aacgagccag
    17821 tcgtgccaac gatataacca attgcgattg gcgtgacgat gccagaaatg ttaccgaaca
    17881 tgttgaacag gccaccggaa agaccgctga tctcttttgg cgcggtatct gccattactg
    17941 cccaacccag cgccccgatg cccttaccga agaaggccag cgccataaag ccgatgatca
    18001 tccactcaac gttgacgtag ttgcagaaca ccatcaccat cgacagcaac atgcccatta
    18061 cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg gcgcatcagc cagtcggaaa
    18121 taatcccacc cagcacacca ccgataaaac cacaaaccgc tggaacggaa gccacaaagc
    18181 ccgctttcag aatcgacatc ccacgcgcct gcaccagata aaccggaaac caggtaatga
    18241 aaaagtaagt cagggcgttg atacagtact gaccgatata aacgccgatc atcatccgtg
    18301 acccgagcag ttgtttgatc tgcccccact tcacgctgaa cggcacttta actttggtgt
    18361 tttgctgatc catattgatc agcgcgccac ccgcggcgat gtactccagc tctttctgat
    18421 ttacacccgg atgttgattt ggctcgtgga tgactttcaa ccagataaag ctgatgacaa
    18481 tccccagacc gcccataaag aagaagacgt gtgaccagcc cacttcatgc gtcagccagc
    18541 ccataatcgg cgcgaagatc accgttgcga agtattgagc ggagttaaaa atcgacaccg
    18601 ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat gcgactgttg ccggggaaag
    18661 atggcgcttc agcaagcccg accaggaagc gcagagtaaa cagggcaacg ataatgccga
    18721 atccactaaa gatatcgacg aagccttgca gcaaggtaaa catcgaccag ataaagatcg
    18781 accagaagta gacgcgtttt gaaccaaaac gatccagcaa ccaaccacca gggatctgcc
    18841 cgataacata agcccatgag aaggcagaga acacatagcc cattcccacg ggatcaaggc
    18901 cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt agcgcggtcg ccgtagttga
    18961 aggatgtgac gataaacaac atcaccacta tccagtaacg agcattcgtg cgtttttcca
    19021 cacggctcgc agcctgactt aaagaactca ttgttgcact cctgaaaatt cgcgttagcc
    19081 acgctcactc tggactgcga catcgccagg aaatcagagg tgacgtaggg tgttttttgc
    19141 cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat agcagtataa
    19201 aaagcgcgcc atagcggctc accgtgcaac aacacaacat taatgcgttc aatgaggccc
    19261 gattttggca ttagccctgg acggtggaat ccacttcacg gaaatgaaaa caagaacaag
    19321 aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa atggatcgct tgactccagg
    19381 caaacgccag taaaaatccg cgctatgaag cagtttttac tggcatttgc ctgaaaagat
    19441 tcgattcagc accgctaaaa cgacatttac cgctcgctga acatatcagg acaacagcgt
    19501 gccccactgt tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    19561 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    19621 tttgccgcca ttgcagaaat ttacataact gctgtcgccg agcgcaatca cgccataacg
    19681 cagattcggc tggaagccca gactatcttt gattccctga aagagcggca caatgctatc
    19741 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    19801 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgtggtcag
    19861 aattgcttct gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat
    19921 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    19981 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt
    20041 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta
    20101 aaaggatgcg tcaatgacaa ctgactggca tgcagcatta accgctgaag accaaaatgc
    20161 tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga
    20221 cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc
    20281 accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc
    20341 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    20401 gcgattttgt ccagttcttc caccagtgga taatccagca ccgcttcttc catcaaccag
    20461 ccgcgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    20521 agccgtccgg cctcgctgga taatcccatc aacaacacac cagaagttgg tcggtccaga
    20581 cgatgagcag taaaaacatg ctgtcctatc tggtcacgca cggtttgcat gaccactact
    20641 ttctcgtcgc gatccagcca gctgcggtga accagccagc cggagggttt atttaccgca
    20701 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    20761 catccagttt ttccaactca gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg
    20821 ccatttcata atagggtgca acagcaaaag cgcccggtaa cggctggttg ttatttaaca
    20881 aatcgtgcat tcgcgggatc agcacccact gcaaccactc cagtggttcc atggtgtcca
    20941 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    21001 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    21061 tcataaagca acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga
    21121 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttattgctcc
    21181 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    21241 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    21301 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    21361 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    21421 cctgcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    21481 tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    21541 ttcatatgaa agttaagaat taacaagcaa catgttgttt ttaaatgaaa ataacgattc
    21601 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    21661 ctcacaacca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    21721 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctcttta cacacttcac
    21781 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    21841 tgggtggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    21901 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    21961 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa
    22021 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    22081 agtatacggc atcttcagta gttgaaataa tgcacggtga aggaacgccg tacagttcct
    22141 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    22201 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    22261 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    22321 aggcacttgc gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca
    22381 gcctactgca aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    22441 acctgccttt tcaaggtgcg gacatctgga cgctgtatga actttcctgg ctgaatgcga
    22501 aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    22561 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    22621 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgctcag ggtgaggtta
    22681 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    22741 cttgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    22801 agaatgccac cagtggtgaa aaagtagtgg aagagacgct tgtcagccac ctgctgaaat
    22861 caaactgcct gatcacccat caaccagatt ggggttcgat ccaaattcag tatcgtggac
    22921 gccaaattga cagagaaaaa ctgctgcgtt acctggtctc tttccgccat cacaacgagt
    22981 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    23041 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    23101 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    23161 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    23221 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    23281 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    23341 gcgacctcta tcaattgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    23401 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcttgcgcc
    23461 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    23521 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgagacatt ctcttcgttt
    23581 acgggcaaat ccataatacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    23641 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc actgcatgtg ggtgaagcgc
    23701 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    23761 gtcgcgtcgg aaaccagcta ggcctgcgtg agctgaatat ctgcaccggc tgtggtccgg
    23821 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    23881 acagtcgttt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    23941 tggtcaacga attgatcatc atgccggata tcgaaaaacg tctggaagcg tttgtccgta
    24001 tcgctcacgg catcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt
    24061 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    24121 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgtg catacgctgg
    24181 gtgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    24241 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg cgacactggt gacgcttaca
    24301 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    24361 agaatatggc taatctgaag ctttacccgg atcaacctgt tgaagtgctg gctgccgacc
    24421 tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca
    24481 ttgaagagtt tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg
    24541 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    24601 cttgctacga aatctgcacg taatctcccc tcccccggtg acgtttgttg ccgggttttc
    24661 tcgtttttgg tcacttactc atcaactcat ttcatttgtt atatgaatgt ttcttaccac
    24721 cctcacgcga caaatatcat cacagttaat atgtcataca atttatattg caacgcaaac
    24781 gtttccctat ttt
//
LOCUS       DAZUGK010000050        24688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZUGK010000050 DAZUGK010000000
VERSION     DAZUGK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.050"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            401..1540
                     /gene="wza"
                     /locus_tag="ACHY5W_001804"
     CDS             401..1540
                     /gene="wza"
                     /locus_tag="ACHY5W_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFS4446712.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVYVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1546..1989
                     /gene="wzb"
                     /locus_tag="ACHY5W_001805"
     CDS             1546..1989
                     /gene="wzb"
                     /locus_tag="ACHY5W_001805"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFS4446713.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1992..4154
                     /gene="wzc"
                     /locus_tag="ACHY5W_001806"
     CDS             1992..4154
                     /gene="wzc"
                     /locus_tag="ACHY5W_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFS4446714.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLTKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDQDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLALGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4332..5171
                     /gene="wcaA"
                     /locus_tag="ACHY5W_001807"
     CDS             4332..5171
                     /gene="wcaA"
                     /locus_tag="ACHY5W_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFS4446715.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5174..5662
                     /gene="wcaB"
                     /locus_tag="ACHY5W_001808"
     CDS             5174..5662
                     /gene="wcaB"
                     /locus_tag="ACHY5W_001808"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFS4446716.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSMWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5659..6876
                     /gene="wcaC"
                     /locus_tag="ACHY5W_001809"
     CDS             5659..6876
                     /gene="wcaC"
                     /locus_tag="ACHY5W_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFS4446717.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDATLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDVATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALLFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6851..8068
                     /gene="wcaD"
                     /locus_tag="ACHY5W_001810"
     CDS             6851..8068
                     /gene="wcaD"
                     /locus_tag="ACHY5W_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFS4446718.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLIIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKRLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8079..8825
                     /gene="wcaE"
                     /locus_tag="ACHY5W_001811"
     CDS             8079..8825
                     /gene="wcaE"
                     /locus_tag="ACHY5W_001811"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFS4446719.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8841..9389
                     /gene="wcaF"
                     /locus_tag="ACHY5W_001812"
     CDS             8841..9389
                     /gene="wcaF"
                     /locus_tag="ACHY5W_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFS4446720.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETK"
     gene            9416..10537
                     /gene="gmd"
                     /locus_tag="ACHY5W_001813"
     CDS             9416..10537
                     /gene="gmd"
                     /locus_tag="ACHY5W_001813"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFS4446721.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKAIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10540..11505
                     /gene="fcl"
                     /locus_tag="ACHY5W_001814"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10540..11505
                     /gene="fcl"
                     /locus_tag="ACHY5W_001814"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFS4446722.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNESYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11508..11987
                     /gene="gmm"
                     /locus_tag="ACHY5W_001815"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11508..11987
                     /gene="gmm"
                     /locus_tag="ACHY5W_001815"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFS4446723.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFTVAEEELLLPDEQHDDYRWLTSDALLASDNVHANSRAYFLAEKRTGVPGL"
     gene            11984..13207
                     /gene="wcaI"
                     /locus_tag="ACHY5W_001816"
     CDS             11984..13207
                     /gene="wcaI"
                     /locus_tag="ACHY5W_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFS4446724.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCAPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVAEADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13210..14646
                     /gene="cpsB"
                     /locus_tag="ACHY5W_001817"
     CDS             13210..14646
                     /gene="cpsB"
                     /locus_tag="ACHY5W_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFS4446725.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14839..16209
                     /gene="cpsG"
                     /locus_tag="ACHY5W_001818"
                     /gene_synonym="manB"
     CDS             14839..16209
                     /gene="cpsG"
                     /locus_tag="ACHY5W_001818"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFS4446726.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLSQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16343..17737
                     /gene="wcaJ"
                     /locus_tag="ACHY5W_001819"
     CDS             16343..17737
                     /gene="wcaJ"
                     /locus_tag="ACHY5W_001819"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFS4446727.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17739..19217
                     /gene="wzxC"
                     /locus_tag="ACHY5W_001820"
     CDS             17739..19217
                     /gene="wzxC"
                     /locus_tag="ACHY5W_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFS4446728.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLKNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPSLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19289..20569
                     /gene="wcaK"
                     /locus_tag="ACHY5W_001821"
     CDS             19289..20569
                     /gene="wcaK"
                     /locus_tag="ACHY5W_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFS4446729.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCSFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMRMVQSVLERIGEAK"
     gene            20566..21786
                     /gene="wcaL"
                     /locus_tag="ACHY5W_001822"
     CDS             20566..21786
                     /gene="wcaL"
                     /locus_tag="ACHY5W_001822"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFS4446730.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIESCRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21797..23191
                     /gene="wcaM"
                     /locus_tag="ACHY5W_001823"
     CDS             21797..23191
                     /gene="wcaM"
                     /locus_tag="ACHY5W_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFS4446731.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23366..24259
                     /gene="galF"
                     /locus_tag="ACHY5W_001824"
     CDS             23366..24259
                     /gene="galF"
                     /locus_tag="ACHY5W_001824"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFS4446732.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAGVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     LVLAKRMAGDLSEYSVIQTKETLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGTKFRKSIEMLLEK"
     gene            24590..>24688
                     /locus_tag="ACHY5W_001825"
     CDS             24590..>24688
                     /locus_tag="ACHY5W_001825"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4446733.1"
                     /translation="MKQKLIIIGAGGFAKTVIDSLDHEKYEIEGFID"
BASE COUNT         6045 a         6167 c         6723 g         5753 t
ORIGIN      
        1 atagccaatt gccgaattgt taccttgcct gctattccgt tagctgtaac acttcctcct
       61 gcattattgg agagccaata ttcaattacc ataatgtcac cttatattcg gaagttaatt
      121 tccgcaataa ccctttctca tcaacgattg tatgcagaga gggtttgaaa aattaagcta
      181 aatcagtgta ttggtagcta aaaagccagg ggcggtagcg tgtctggatg cctgaaagac
      241 cagtctgaat tattctgtca atagcctgcg gatgaaatca atttttttag gactgatgcc
      301 agttaaattt gtattcaatt aactgcaagg taaatgtgca tggcgacatt gctattaata
      361 gtgcacagga taattactct gccaaagtga taaataaaca atgatgaaat ccaaaatgaa
      421 attgatgcca ttattggtgt cagtaacctt gataagcggt tgcacagtac ttccgggcag
      481 caatatgtcg acgatgggca aagacgtcat caaacagcag gacgctgatt tcgatctcga
      541 caaaatggtg aatgtttatc cgttgacccc gcgcctgatt gaccaattac gtccacgacc
      601 caacgtagcg cgccccaata tgacgctgga aagcgagatc gcgaattacc agtatcgcgt
      661 cgggccgggg gacgttctta atgtcaccgt ctgggatcac ccggaactca ccacgccagc
      721 cggtcagtac cgcagctcca gtgacaccgg caactgggta cagcctgacg gcaccatgtt
      781 ctacccgtat atcggcaagg tttacgtagt cgggaaaacg ctcgctgaaa tccgcagtga
      841 tattaccggg cgcttagcga cgtacatcgc tgacccgcag gtggacgtta atatcgccgc
      901 cttccgctca caaaaggcct atatctcagg tcaggtgaat aaatccggtc aacaggcgat
      961 caccaacgta ccactgacta ttctcgacgc catcaacgcc gcaggtggcc tgaccgacac
     1021 cgctgactgg cgcaacgtgg tgctaacaca caatggtcgt gaagaacgca tttctttgca
     1081 ggcgctgatg caaaacggcg acctcaatca gaaccgactg ctttatcccg gcgatattct
     1141 ctacgtgccg cgtaatgatg atctgaaagt atttgtgatg ggtgaagtga agaaacagag
     1201 caccctgaaa atggacttta gcggcatgac cctgactgaa gccctgggta atgctgaagg
     1261 catcgacatg accacctcca acgccagcgg catctttgtc attcgtccgc tgaaaggcga
     1321 gggcgggcgt aatggcaaga ttgccaatat ctaccagctg gatatgtccg atgcgacgtc
     1381 gctggtgatg gcgacagaat tccgcctgca accttatgac gtggtgtatg tcaccaccgc
     1441 cccggtttcc cgctggaacc gtctgatcaa tcagttgctg ccaactatta gcggtgtccg
     1501 ttacatgacg gatacagcca gcgacattca taactggtaa tcgtcatgtt taacaacatc
     1561 ttagttgtct gtgtcggcaa tatttgccgt tccccgacgg cggaacgctt actgcaacgt
     1621 tatcacccgg aactgaaagt ggagtccgct ggactcggcg cgctggtcgg taagggcgct
     1681 gatcctaccg ctatcagcgt cgccgcagaa catcaactgt ctctggaagg tcactgtgca
     1741 cggcaaatca gccgccgcct gtgtcgcaac tatgacctga ttttgaccat ggaaaagcgc
     1801 catatcgaac gcttatgcga gatggcacct gagatgcgcg gcaaagtgat gctgtttggt
     1861 cactgggata acgaatgtga aatccccgat ccgtatcgca aaagccggga aacgtttgca
     1921 gcggtgtaca cattacttga acggtctgcc cgccagtggg cgcaggcatt gaacgcagag
     1981 caggtataag aatgacagaa aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg
     2041 aaatcgatat tggtcgcctg gtcggcaccg tcattgaagc gcgctggtgg gtgattggca
     2101 tcaccgccgt attcgccctc tgtgctgtgg tttacacctt cttcgccacg ccgatataca
     2161 gtgccgacgc actggtacaa atcgagcaaa gcagcggcaa ttcgttagtg caggatatcg
     2221 gatcggcatt agccaataaa ccgccagcat cggatgccga gatccagttg attcgttcgc
     2281 gtctggtgct tggtaaaacg gtggacgatc tagacctcga tattgcggtg agcaaaaaca
     2341 cgttcccgat tttcggtgcg ggttgggatc gcctgatggg acgtcagaac gagacggtga
     2401 aagtgaccac ctttaaccgc ccgaaagaga tggcggacca ggtgtttacg cttaatgtgc
     2461 tggatgacaa aaactacact ctgagcagtg atggcggctt tagcgcccgt gggcaagcgg
     2521 gccagatgct gacaaaagaa ggcgtcacgc tgatggttga agctattcac gcccgcccgg
     2581 gcagtgagtt taccgtcacc aaatactcca cgctggggat gatcaaccaa ctgcaaaaca
     2641 gcctgacggt aacggagaac ggcaaagacg caggcgtttt gagcctgact tataccggtg
     2701 aagatcgcga acagatccgc gacattctta acagcatcgc ccgtaactat caggaacaaa
     2761 atattgagcg caaatcggcg gaagcgtcga aaagcctcgc tttcctcgcg caacagttac
     2821 cggaagtacg tagccgcctc gatgttgccg aaaacaaact gaatgccttc cgtcaggatc
     2881 aagattctgt tgatctgccg ctggaagcga aagcggtgct cgattcgatg gtgaacatcg
     2941 acgcacaatt gaacgaactg acctttaaag aagcggaaat ctccaagctg tacaccaaag
     3001 ttcacccggc gtatcgcacg ctgctggaga aacggcaggc gctggaagac gaaaaagcca
     3061 aacttaacgg tcgcgtaacg gcgatgccga aaacccagca ggaaattgtc cgtctgaccc
     3121 gcgatgtcga gtctggtcag caggtctata tgcaactgct gaataaagag caggagctga
     3181 aaatcaccga agccagcacc gtcggcgatg tgcgcattgt tgacccggca atcacccagc
     3241 ctggcgtgct aaaaccgaag aaagggctga ttatcctcgg cgcgattatc cttggcctga
     3301 tgctctctat cgtgggtgtg ctgctgcgct cgttgtttaa tcgcggcatc gaaagcccgc
     3361 aggtgctgga agaacacggt atcagcgtct atgccagcat cccgctgtcg gagtggcaga
     3421 aagcgcgcga tagcgtcaaa accatcaaag gggttaaacg ctataagcag agccagctac
     3481 tggcgttggg gaatccaacc gatctggcga ttgaagccat ccgcagtctg cgtaccagtc
     3541 tgcacttcgc gatgatgcag gcgcagaaca atgtgttgat gatgacaggg gttagcccgt
     3601 caatcggtaa aacctttgtc tgcgccaacc tggcggcggt gatcagtcag accaataaac
     3661 gggtgttgtt gatcgactgc gatatgcgca aaggctacac ccacgagctg ttgggcacta
     3721 ataacgtcaa tggcctgtcg gaaattctga ttggtcaggg cgatattact accgctgcta
     3781 aaccgacctc tattgccaaa tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt
     3841 ctgaactgtt gatgagcgaa cgctttgccg aactggtgaa ctgggcgagt aaaaactatg
     3901 acctggtgtt gattgatacg ccgccgattc tggcagtgac cgatgcggca attgttggtc
     3961 gtcatgttgg aaccacgtta atggtggcgc gttatgcggt caacacattg aaagaagtgg
     4021 aaaccagtct gagccgcttt gagcaaaacg gtattccggt gaaaggggtg attctgaact
     4081 ccatcttccg ccgcgccagc gcgtatcagg attatggcta ttacgaatac gaatataagt
     4141 cggatgcgaa ataacgacat ccgctgtcgg atgcgatgct gacgcatctt atccgaccta
     4201 cgctcacgcc gcatccggca agcaaaccag ctccgtaggc cggataaggc gctcacgccg
     4261 catccggcaa cagaacccgt aaacaagctt ataaaccggc agaacatctc gacactccgg
     4321 gagaacaacc tatgaaagac aatccgctga tctcaatcta tatgccgacc tggaaccgcc
     4381 aacaactggc gattcgggcg ataaaatcgg tcttgcgcca ggactacagc aactgggaga
     4441 tgatcatcgt ggatgattgc tccacctcct gggaacagct gcaacagtac gtcaccgccc
     4501 tcaacgatcc gcgtatcacc tacattcaca acgacattaa cagcggggcg tgcgcggtac
     4561 gtaaccaggc gattatgctg gcgcaagggg aatacatcac cgggatcgat gatgatgatg
     4621 aatggacgcc caaccgcctg agcgtcttcc tcgcccataa acagcaactg gtcacacacg
     4681 cctttttgta cgctaacgac tacgtttgcc agggagaagt ttattcccaa ccggcaagct
     4741 taccactgta tcccaaatcg ccgtactcac gccgcctgtt ctataaacgc aatatcattg
     4801 gcaatcaggt ctttacctgg gcatggcgtt tcaaagagtg tttgttcgat accgaactga
     4861 aagcagcaca ggattacgac atcttcctgc ggatggtggt ggagtacggc gaaccgtgga
     4921 aagtggaaga ggcgacgcag atcctgcata tcaatcacgg tgaaatgcag atcacctcgt
     4981 caccgaaaaa attctccgga tacttccact tttaccgcaa gcacaaagac aaattcgacc
     5041 gtgccagtaa aaaatatcag ctgtttaccc tctaccagat ccgcaataaa cgtatgacct
     5101 ggcgcacttt gctgacgctg ctgtcggtgc gtaacggcaa acgtctggct gatggtattc
     5161 gggggcgcta aagatgctgg aagatctacg cgccaacagc tggagtttac gcccgtgctg
     5221 catggttctt gcctatcgcg tcgctcattt ttgttcgatg tggcgcaaaa agaacgtcct
     5281 caacaatctg tgggcggccc cgctgctggt gctgtatcgc attatcaccg aatgcttttt
     5341 cggttatgaa atccaggcag ccgcgaccat tggccgccgc tttactatcc atcacggtta
     5401 cgccgtagtg atcaataaaa acgtggtagc gggggatgat ttcaccattc gccacggcgt
     5461 cactatcggc aatcgtggtg ccgataacat ggcatgtcca cacattggca acggcgtcga
     5521 actcggtgcc aacgtcatta ttcttggtga tatcacgctt ggtaacaacg tcaccgtggg
     5581 cgcgggcagc gtggtgctcg actctgtccc ggacaacgcg ctggtggtgg gagaaaaagc
     5641 gcgagtgaag gtaattaaat gaatattttg caatttaatg tgcgactggc ggaaggcggg
     5701 gcagcaggtg tggcgttaga tctccaccag cgtgcgctgc aacaggggct ggcgtcacat
     5761 tttgtctatg gctacggcaa aggcggcaaa gagagcgtca gccatcagaa ctatccgcag
     5821 gtcatcaaac atacgccgcg gatgaccgcg atggcgaata ttgccctgtt tcgtctgttt
     5881 aatcgcgatc tgttcggcaa tttcaatgag ttatatcgca ccattactcg cacaccgggt
     5941 ccggtggtcc tgcattttca tgtgctgcac agctactggt tgaatcttaa cagcgtggtg
     6001 cgcttttgcg aaaaggtgaa aaaccataaa ccggacgcca ctctggtctg gacgctgcac
     6061 gatcactgga gcgttaccgg acgctgcgcc tttaccgacg gttgcgaagg ctggaaaacg
     6121 ggctgccaga aatgcccgac cttaaataac tatccgccgg tgaagattga tcgggcgcac
     6181 cagctggtgg cgggcaaacg ccagttattc cgcgagatgc tggcactggg ctgtcagttt
     6241 atttccccca gccagcatgt ggctgacgct ttcaatagtc tgtacggtcc agggcgttgc
     6301 cggattatca ataatggtat tgatgtggca acagaagcga ttctggcgga cttgcctccg
     6361 gtacgcgaaa cccagggcaa gccgaaaatc gcggtggtgg cgcatgacct gcgttacgac
     6421 ggcaagacta accagcaact ggtgcgtgag atgatggcgc tgggcgataa aatcgaactg
     6481 catacctttg gtaagttctc gccgttcacc gctggcaacg tggttaatca cggctttgaa
     6541 actgacaagc gcaagttgat gagcgcgctc aatcagatgg atgcgctgct gttcagttct
     6601 cgcgtcgata actacccgct gattttgtgt gaggcgctat cgattggcgt gccggtgatt
     6661 gccacccata gcgacgcggc gcgggaagtg ctgcaaaaat ccggcggtaa aaccgtcagc
     6721 gaagaagacg tgctgcaact ggtgcagtta agcaaaccgg aaatcgcgca ggcgatattt
     6781 ggtaccacgc tggctgagtt cagccaacgc agccgcgccg cctacagtgg acaacagatg
     6841 ctggaggagt atgtcaactt ctatcagaat ctgtagctac ctgctgctgc cgctgatcta
     6901 cctgctggtt aacgtcaaaa tcgcccagct tggcgaaagt ttccccatca ccatcgtcac
     6961 ttttttacct gtcttattac tgctgttttt agaacgcatc agcgttaaaa aattgattat
     7021 tgccttaggg attggtgcgg gactcacggc gtttaactac ctgtttggtc agtcgttgga
     7081 tgccagtaaa tacgtcactt caaccatgct gtttgtctat attgtgatca ttattggcat
     7141 ggtgtggagt attcgcttta aaacgatttc gccacacaac catcgcaaga tattacgttt
     7201 cttttatctg gtggtcgggc tggtggtggc gctggcggcg gtggagatgg cgcaaattat
     7261 cctcaccggt ggcagcagta ttatggagtc gatttcgaaa tatctgattt acagcaacag
     7321 ctatgtactg aatttcatta aattcggcgg caagcgcacg acagcacttt atttcgaacc
     7381 ggcatttttc gctctggcat taatctcaat ttggctcagc atcaaacagt ttggtatcaa
     7441 aacgcctaaa accgatgcta tgattctcgc agggataata ttatccggat cgttttcagg
     7501 ggttatgacc tttatcctgt tttatttgct ggagtgggca ttccaatatc tgaataaaga
     7561 ggcgattaag aaaaggttac cgttagcatt gatttctctg gctgtattcc tggttggtgt
     7621 ggtaatcgcg tttccttata tttccacccg tctgggcgat ttaggtacgg aaggatcgtc
     7681 atcgtattat cgtattgtcg gtccgctggt gatggtcggt tattctttga cccatattga
     7741 cggtgtagtc agatttggct cactttatga atatgtcgca tcattcggaa tatttaacgg
     7801 tgcggatgtc ggaaaaacca tagacaatgg tttgtatctg ctgattattt atttttcctg
     7861 gttcgcggtg tttttatcgc tgtggtacat ggggaaagtg ataaaaatga tgatcaacgc
     7921 ttttggtgat aaccgcaatt ttcgcgtgca attatatctt tttactccgg tatcgctgtt
     7981 ttttaccggt tcgatattta gcccggaata tgcattttta atcgtctgtc cgtttatttt
     8041 gcgaaaagcg ttaaatatta cgaggtaaga ataagaacat gttgcttagc ataatcactg
     8101 tcgcgtttcg taacctcgaa gggatagtca aaacacatgc ctcgctggcg catctggcgc
     8161 aggcggacga tatcagcttc gaatggattg ttgtcgatgg cggctccaat gacggcaccc
     8221 gtgagtatct ggaaaatctc aatggtatct ataacctacg ctttgtcagc gagccagata
     8281 acggtatcta cgacgccatg aacaagggca ttgcgatggc acaaggcaag ttcgcgttgt
     8341 ttctcaactc gggcgatatt tttcatcagg atgccgcata ttttgtccgt aagttaaaaa
     8401 tgcaaaaaga taacgtgatg atcaccggcg atgcgctgct ggattttggc gacgggcata
     8461 aaattaaacg tagcgccaaa ccgggttggt atatttatca cagcctgccc gccagtcatc
     8521 aggcgatatt tttcccggta tccggtttga aaaaatggcg ttatgacctg gaatataaag
     8581 tttcttctga ttacgcgctg gcagccaaaa tgtataaagc aggttatgca tttaaaaaac
     8641 tcaatggcct ggtgtctgaa ttttctatgg gtggggtatc taccaccaat aatatggaat
     8701 tgtgtgctga cgcgaaaaaa gtccaacggc aaatattaca tgtgcctggc ttctgggcag
     8761 aattatcctg gcatttacgc caacgtacta cctcaaagac gaaagcctta tataacaaaa
     8821 gctgaatata agggaaaacg atgcaagatt taagcggttt ctcggtgccg aaagggttcc
     8881 ggggcggcaa cgctattaaa gtgcaattat ggtgggcagt acaggcaaca atatttgcct
     8941 ggtcgccaca agtattgtat cgctggcggg cttttttatt acgtttattc ggcgcaaaaa
     9001 taggaaaaaa cgtagttatt cgtccgtcag taaaaattac ctatccgtgg aaattaacct
     9061 taggtgatta cgcgtgggtc ggcgatgacg tcaatttata taccctcggt gaaataacca
     9121 ttggcgcaca ttcggtgata tcgcaaaaaa gttatttatg caccggtagc cacgaccatg
     9181 caagtcaaca tttcaccatt aatgccacgc ctattgtgat tggcgagaaa tgctggctgg
     9241 caaccgatgt ttttgttgcc cctggcgtca cgattggcga cggcaccgtc gtgggtgcac
     9301 gaagcagtgt ttttaaatcg cttccggcaa atgtggtttg ccgggggaat cccgcagtgg
     9361 tgatacgcga acgcgttgaa actaaataaa ttcaaaaaat acagaggaat aagacatgtc
     9421 aaaagtcgct ctcatcaccg gtgtaaccgg acaagacggt tcttacctgg cagagtttct
     9481 gctggaaaaa ggttacgagg tgcatggtat taagcgtcgt gcatcgtcat tcaacaccga
     9541 gcgcgtggat cacatttatc aggatccgca cacctgcaac ccgaaattcc atctgcatta
     9601 tggcgacctg agtgatacct ccaacctgac gcgcattttg cgtgaagtgc agccggatga
     9661 agtgtacaac ctgggcgcaa tgagccacgt tgcggtttct tttgagtcac cggaatatac
     9721 cgctgacgtc gacgcgatgg gtacgctgcg cctgctggag gcgatccgct tcctcggtct
     9781 ggaaaagaaa acccgtttct atcaggcttc cacctctgaa ctgtacggtc tggtgcagga
     9841 aattccgcag aaagaaacca cgccgttcta cccgcgatct ccgtatgcgg tcgccaaact
     9901 gtatgcctac tggatcaccg ttaactaccg cgaatcctac ggcatgtacg cctgtaacgg
     9961 tattctcttc aaccatgaat ccccgcgccg cggcgaaacc ttcgttaccc gcaaaatcac
    10021 ccgcgcaatc gccaacatcg cccaggggct ggagtcgtgt ctgtacctcg gcaatatgga
    10081 ttccctgcgt gactggggcc acgccaaaga ctacgtaaaa atgcagtgga tgatgctgca
    10141 acaggaacag ccagaagatt tcgttatcgc gaccggcgtt cagtactccg tacgtcagtt
    10201 cgtggaaatg gcggcagcac agctgggcat caaactgcgc tttgaaggca cgggcgttga
    10261 agagaaggcc attgtggttt ccgtcaccgg gcatgacgcg ccgggcgtta aaccgggcga
    10321 tgtgattatc gccgttgacc cgcgttactt ccgtccggcg gaagttgaaa cgctgctcgg
    10381 cgacccgacc aaagcgcacg aaaaactggg ctggaaaccg gaaatcaccc tcagagagat
    10441 ggtgtctgaa atggtggcta atgacctcga agcggcgaaa aaacactctc tgctgaaatc
    10501 tcacggctac gacgtggcga tcgcgctgga gtcataagca tgagtagaca acgcattttt
    10561 atcgctggcc atcgcggaat ggtcggttcc gccatcacgc ggcagctcga acagcgcggt
    10621 gatgtggaac tggtattacg cacccgcgac gagctgaacc tgctggacag ccgcgcggtg
    10681 catgatttct ttgccagcga acgcattgac caggtctatc tggcggcggc gaaagtgggc
    10741 ggcattgttg ccaacaacac ctatccggcg gatttcatct accagaacat gatgattgag
    10801 agcaacatca ttcacgccgc gcatcagaac gacgtgaaca aactgctgtt tcttggatcg
    10861 tcctgtatct acccgaaact ggcaaaacag ccgatggcag aaagcgagtt attgcagggc
    10921 acgctggagc cgaccaacga gtcttacgcc attgccaaaa tcgccgggat caaactgtgc
    10981 gaatcataca accgccagta tggacgcgat taccgctctg tcatgccgac caacctgtac
    11041 gggccgcatg acaacttcca cccgagtaac tcgcatgtga tcccagcatt gctgcgtcgc
    11101 ttccacgagg cgacggcaca gaatgcgccg gacgtggtgg tatggggcag cggtacaccg
    11161 atgcgtgaat tcctgcacgt cgatgacatg gcggcggcga gcattcatgt gatggagctg
    11221 gcgcatgaag tctggctgga gaacacccag ccgatgctgt cgcacattaa cgttggtacg
    11281 ggcgttgact gcactatccg cgagctggcg caaaccatcg ccaaagtggt gggttacaaa
    11341 ggtcgggtgg tttttgatgc cagcaaaccg gatggtacgc cgcgcaaact gctggatgtg
    11401 acgcgcctgc atcagcttgg ctggtatcac gaaatctcac tggaagcggg gcttgccagc
    11461 acttaccagt ggttccttga gaatcaagac cgctttcggg ggtaatgatg tttttacgtc
    11521 aggaagactt tgccacggta gtgcgctcca ccccgcttgt ctctctcgac tttattgtcg
    11581 agaacagtcg cggcgagttt ctgcttggca aaagaaccaa ccgcccggcg cagggttact
    11641 ggtttgtgcc gggagggcgc gtgcagaaag acgaaacgct ggaggccgca tttgagcggc
    11701 tgacgatggc ggaactgggg ctgcgtctgc cgataacagc aggccagttt tacggtgtct
    11761 ggcagcactt ttatgacgat aacttctctg gcacggattt caccactcac tatgtggtgc
    11821 tcggttttcg cttcacggta gcggaagaag agctgttact gccggatgag cagcatgacg
    11881 attaccgctg gctgacgtcg gacgcgctgc tcgccagtga taatgttcat gctaacagcc
    11941 gcgcctattt tctcgctgag aagcgtaccg gagtacccgg attatgaaaa tactggtcta
    12001 cggcattaac tactcgccgg agttaaccgg catcggcaaa tacaccggcg agatggtgga
    12061 atggctggcg gcacaaggtc atgaggtgcg ggtcattacc gcaccgcctt actacccgca
    12121 atggcaggtg ggcgagaact attccgcctg gcgctacaaa cgagaagagg gggccgccac
    12181 ggtgtggcgc tgcccgctgt atgtgccaaa acagccgagc accctgaaac gcctgttgca
    12241 tctgggcagt tttgccgtca gcagtttctt tccgctgatg gcgcaacgtc gctggaagcc
    12301 ggatcgcatt attggcgtgg tgccaacgct gttttgcgcg ccgggaatgc gcctgctggc
    12361 gaaactctct ggtgcgcgta ccgtgctgca tattcaggat tacgaagtgg acgccatgct
    12421 ggggctgggc cttgccggaa aaggcaaagg cagcaaagtg gcacagctgg caacggcgtt
    12481 cgaacgtagc ggactgcata acgtcgataa cgtctccacg atttcgcgtt cgatgatgaa
    12541 taaagccatc gaaaagggcg tggcggcgga aaacgtcatc ttcttcccca actggtcgga
    12601 aattgcccgt tttcagcatg tcgcagaggc cgatgttgat gcccttcgta accagcttgg
    12661 cctgccggat aacaaaaaaa tcattcttta ctccggcaat attggtgaaa agcaggggct
    12721 ggaaaacgtt attgaagctg ccgatcgcct gcgcgatgaa ccgctgattt ttgccattgt
    12781 cgggcagggc ggcggcaaag cgcggctgga aaaaatggcg caacagcgtg gtctgcgcaa
    12841 catgcaattt tttccgctgc aatcgtatga cgctttaccc gcactgctga agatgggcga
    12901 ttgccatctg gtggtgcaaa aacgcggcgc ggcagatgcc gtattaccgt cgaaactgac
    12961 caatattctg gcagtaggcg gtaacgcggt gattaccgca gaagcccaca cagaactggg
    13021 acagctttgc gaaacctttc cgggcattgc ggtttgcgta gaaccggaat cggtcgaggc
    13081 gctggtggcg gggatccgtc aggcgctcct gctgcccaaa cacaacacgg tggcacgtga
    13141 atatgccgaa cgcacgctcg ataaagagaa cgtgttacgt caatttataa atgatattcg
    13201 gggataatta tggcgcagtc gaaactctat ccagttgtga tggcaggtgg ctccggtagc
    13261 cgcttatggc cgctttcccg cgtactttac cccaagcagt ttttatgcct gaaaggcgat
    13321 ctcaccatgc tgcaaaccac catctgccgc ctgaacggcg tggagtgcga aagcccggtg
    13381 gtgatttgca atgagcagca ccgctttatt gtcgcggaac agctgcgtca actgaacaaa
    13441 ctcaccgaga acattattct cgaaccggca gggcgtaaca ctgcacctgc cattgcactg
    13501 gcggcgctgg cggcaaaacg tcatagcccg gagagcgacc cgttaatgct ggtgctggcg
    13561 gcggatcatg tgattgccga tgaagacgcg ttccgtgccg ccgtgcgtaa tgccatgccg
    13621 tatgccgaag cgggcaagct ggtgaccttc ggcattgtgc cggatctacc tgaaaccgga
    13681 tatggctata ttcgtcgcgg tgaagtgtcg gcgggtgagc aggatacggt ggcctttgaa
    13741 gtggcgcagt ttgtcgaaaa accgaatctg gaaaccgccc aggcctatgt ggcaagcggc
    13801 gaatattact ggaacagcgg catgttcctg ttccgcgccg gacgctatct cgaagaactg
    13861 aaaaaatatc gcccggatat tctcgatgcc tgtgaaaaag cgatgagctc cgtcgatccg
    13921 gatcttgatt ttattcgtgt ggatgaagaa gcgtttctcg cctgcccgga agagtcggtg
    13981 gattacgcgg tcatggaacg tacggcagat gctgttgtgg tgccgatgga tgcgggctgg
    14041 agcgatgttg gttcctggtc ttcattatgg gagatcagcg cccacaccgc cgagggcaac
    14101 gtttgccacg gcgatgtgat taatcacaaa actgaaaaca gctatgtgta tgccgaatct
    14161 ggcctggtca ccaccgtcgg ggtgaaagat ctggtagtgg tgcagaccaa agatgcggtg
    14221 ctgattgccg accgtaacgc ggtacaggat gtgaaaaaag tggtcgagca gatcaaagcc
    14281 gatggtcgcc atgagcatcg ggtgcatcgc gaagtgtatc gtccgtgggg caaatatgac
    14341 tctatcgacg cgggcgaccg ctaccaggtg aaacgcatca ccgtgaaacc gggcgagggt
    14401 ttgtcggtac agatgcacca tcaccgcgcg gaacactggg tagtggtcgc gggaacggca
    14461 aaagtcacca ttgatggtga tatcaaactg cttggtgaaa acgagtccat ttatattccg
    14521 ctgggggcga cgcactgcct ggaaaacccg gggaaaattc cgctcgattt aattgaagtg
    14581 cgctccggct cttatctcga agaggatgat gtggtgcgct tcgcggatcg ctacggacgg
    14641 gtgtaaacgt cgcatcaggc gctgtttgtc ggatgcggcg tgaacgcctt atccgaccta
    14701 cggttcgggt ttgtagtcct gataagacgc ggcagcgtcg catcaggcaa tgaatgcgaa
    14761 accgagatgt aaataacgac aaaaacaaaa ttggccgttt cggtcagggc caactattgc
    14821 ctgaaaaagg gtaacgacat gaaaaaatta acctgcttta aagcctatga tattcgcggg
    14881 aaattaggcg aagaactgaa tgaagatatc gcctggcgca ttggtcgcgc ctatggcgaa
    14941 tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc gtctcaccag cgaaacctta
    15001 aaactggcgc tggcaaaagg tttacaggat gcgggcgttg acgtgctgga tatcggtatg
    15061 tccggcaccg aagagatcta tttcgccacg ttccatctcg gcgtggatgg cggcattgaa
    15121 gttactgcca gccataatcc gatggattac aacggcatga agctggtgcg cgaaggggct
    15181 cgcccgatca gcggtgatac cggactgcgc gatgtccagc gcctggctga agccaacgac
    15241 tttcctcccg tcgatgaaac caaacgcggt cgctatcagc aaatcaacct gcgtgacgct
    15301 tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc tcacgccgct caagctggtg
    15361 atcaactccg ggaacggcgc agcgggtccg gtggtggacg ccatcgaagc ccgctttaaa
    15421 gccctcggcg cacctgtgga attaatcaaa gtgcacaaca cgccggacgg caatttcccc
    15481 aacggtattc ctaacccgct actgccggaa tgccgcgacg acacccgcaa tgcggtcatc
    15541 aaacacggcg cggatatggg cattgccttt gatggcgatt ttgaccgctg cttcctgttt
    15601 gacgaaaaag ggcagtttat cgaaggctac tacattgtcg gcctgctggc agaagcgttc
    15661 ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac gtctctcctg gaataccgtt
    15721 gatgtggtga ctgccgctgg cggtacgccg gtgatgtcga aaaccggaca cgcctttatt
    15781 aaagaacgta tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac
    15841 ttccgtgatt tcgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg
    15901 gtgtgcctga aaggaaaaac gctgggcgaa ctggtgcgcg accggatggc ggcattcccg
    15961 gcaagcggtg agatcaacag caaactgtca caacccgttg aggcgattaa ccgcgtcgaa
    16021 cagcatttta gccgcgaggc gctggcggtg gatcgcaccg atggtatcag catgaccttt
    16081 gccgactggc gctttaacct gcgctcctcc aacaccgaac cggtggtgcg gttgaatgtg
    16141 gaatcgcgcg gtgatgtacc gctgatggaa gcgcgaacgc gaactctgct gacgttgctg
    16201 aacgagtaat gtcattactt tcccctcacc ctgacgctct actgcctgcg gagagaagtg
    16261 agcacggaac tttcttctcc ccctcgcccc tttgggagag ggccggggtg aggggcgagg
    16321 cgacaataga acaggaacaa cgatgacaaa tctaaaaaag cgcgagcgag cgaaaaccaa
    16381 tgcatcgtta atctctatgg tgcaacgctt ttcagatatc accatcatgt ttgccgggct
    16441 atggctggtt tgcgaagtca gcggactgtc attcctctac atgcacctgt tggtggcgct
    16501 gattacgctg gtggtgttcc agatgctggg cggcatcacc gatttttatc gctcatggcg
    16561 cggtgttcgg gccgcgacag aatttgccct gctgctacaa aactggacct taagcgtgat
    16621 tttcagcgcc ggactggtgg cgttcaacaa tgatttcgac acgcaactga aaatctggct
    16681 ggcgtggtat gggctgacca gcatcggact ggtggtttgc cgttcatgta ttcgcattgg
    16741 ggcgggctgg ctgcgtaatc atggctataa caagcggatg gtcgcggtgg cgggggattt
    16801 agccgccgga caaatgctga tggagagctt ccgtaatcag ccgtggttag ggtttgaagt
    16861 ggtgggcgta taccacgacc caaaaccggg cggcgtttct aacgactggg cgggcaatct
    16921 gcaacaactg gtcgaggatg cgaaagcggg caagattcat aacgtctata tcgcgatgca
    16981 gatgtgcgac ggcgcgcgag tgaaaaaact ggtccatcaa ctggcggaca ccacctgttc
    17041 ggtgctgctg atccccgatg tctttacctt caacattctc cattcccgta tcgaagagat
    17101 gaacggcgta ccggtggtgc cgctgtatga cacgccgctt tccggggtta accgtctgat
    17161 aaaacgtgca gaagacattg tgctggcgac gcttatcctg ctgctgatct ccccggtgct
    17221 gtgctgcatt gcgctggcgg tgaaactgag ctcaccgggg ccggttattt tccgccagac
    17281 tcgctacggc atggatggca agccgatcaa agtgtggaag ttccgttcta tgaaagtgat
    17341 ggagaacgac aaagtggtga ctcaggcgac gcagaacgat ccgcgcgtca ccaaagtggg
    17401 gaactttctg cgccgcacct cgctggatga actgccgcag tttatcaatg tgctgaccgg
    17461 ggggatgtcg attgtcggtc cacgtccgca cgcggtggcg cataacgaac agtatcgaca
    17521 gctcattgaa ggctacatgc tgcgccataa ggtgaaaccg ggcattaccg gctgggcgca
    17581 gattaacggc tggcgtggcg aaaccgacac gctggagaaa atggaaaaac gcgtcgagtt
    17641 cgaccttgag tacatccgcg aatggagcgt ctggttcgat atcaaaatcg ttttcctgac
    17701 ggtattcaaa ggtttcgtta acaaagcggc atattgatat gagcttacgt gaaaaaacca
    17761 tcagcggcgc gaagtggtcg gcgattgcta cggtgatcat catcggcctc gggctggtac
    17821 agatgaccgt gctggcgcgg attatcgaca accaccagtt cggtttgctt accgtgtcgc
    17881 tggtgatcat cgcgctggca gatacgcttt ctgacttcgg catcgctaac tcgattattc
    17941 agcgaaaaga aatcagccac cttgaactca ccacgctgta ctggctgaac gttgggctgg
    18001 ggcttgtggt gtgtgtggcg gtgtttttgt tgagtgatct catcggcgac gtgctgaaga
    18061 atccggacct ggcaccgttg attaaaacat tgtcgctggc gtttgtggtg atcccccacg
    18121 ggcagcagtt ccgcgcgttg atgcaaaagg aactggagtt caacaaaatc ggcatgatcg
    18181 aaaccagcgc ggtgctggcg ggcttcactt ttactgtggt tagcgcccat ttctggccgc
    18241 tggcgatgac tgcgatcctc ggttatctgg tcaatagtgc ggtgagaacg ctgctgtttg
    18301 gctactttgg ccgcaaaatt tatcgcccca gtttgcattt ctcgctggcg tcggtggcac
    18361 cgaacttacg ctttggtgcc tggctgacgg cggacagcat catcaactat ctcaatacca
    18421 acctttcaac gctggtgctg gcgcgtattc tcggcgcggg cgtggcaggg ggatacaacc
    18481 tggcgtacaa cgtggccgtt gtgccgccga tgaagctgaa cccaatcatc acccgcgtgt
    18541 tgtttccggc atttgccaaa attcaggacg acaccgaaaa gctgcgcgtc aacttctaca
    18601 agctgctgtc ggtggtgggg attatcaact ttccggcgct gctcgggctg atggtggtgt
    18661 cgaataactt tgtaccgctg gtctttggtg agaagtggaa cagcattatt ccggtgctgc
    18721 aattgctgtg tgtggtggga ctactgcgct cagtgggtaa cccgattggc tcgctgttga
    18781 tggcgaaagc acgggtggat atcagcttta aattcaacgt attcaaaacc tttctgttta
    18841 ttccggcgat tgttattggt gggcagatgg cgggcgcaat cggcgtcacg ctcggtttcc
    18901 tgctggtgca gattatcaac accattctga gttacttcgt gatgattaaa ccggttcttg
    18961 gttccagtta tcgccagtac atcctgagtt tgtggctgcc gttttatctc tcgctgccga
    19021 cgctggtggt cagttatgcg ctgggcattg tgctgaaagg gcaactggcg ctggggatgc
    19081 tgctggcggt gcaaatagcc gcgggcgtgc tggcgtttgt agtgatgatt gtgctgtcgc
    19141 gccatccgct ggtggtggaa gtgaagcgcc agttttgtcg cagcgaaaaa atgaaaatgc
    19201 ttttacgggc ggggtgagtt gttgttttcg gttccggcaa cgaaaacaac aacataacct
    19261 cacctggcat caaatttatg aggaaaaaat gaaattactt attcttggca accacacttg
    19321 cggcaatcgt ggcgacagcg ccatcttgcg tggcttactt gatgccatca acatcctcaa
    19381 tccacaagcc gaagtggacg tgatgagccg ctatccggtc agttcttcct ggctgctcaa
    19441 ccgcccggta atgggcgatc cgctgttcct gcaaatgaaa caacacaaca gcgcggcggg
    19501 cgttgtcggg cgcgttaaaa aagtcctccg tcgccgctac cagcatcagg tattgctctc
    19561 acgcgtcacc gacactggca agctgcgtaa tatcgccatc gcccagggat tcaccgactt
    19621 cgtgcgcctg ctgtcaggtt acgacgccat tattcaggtc ggcggatcgt tttttgtcga
    19681 tctctacggc gtgccgcagt ttgaacatgc actttgctct ttcatggcga aaaagccgct
    19741 gtttatgatt ggtcacagtg ttggcccgtt ccaggatgag caatttaacc aactggcgaa
    19801 ctacgttttt ggtcactgcg acgcgctgat cctgcgcgaa tcggtcagcc ttgatctgat
    19861 gaaacgcagc aatatcacca ccgcaaaagt ggaacatggc gtcgataccg catggctggt
    19921 cgatcaccac acagaagact tcaccgccag ctatgccgtt caacactggc tggacgttgc
    19981 cgcacaacag aaaacggtag caattaccct gcgcgaactg gcaccgttcg acaaacgtct
    20041 cggcaccact cagcaagcgt atgaaaaagc ctttgccggg gtggtcaatc gcattctcga
    20101 cgaagggtat caggtgattg cgctctccac ctgtacgggc atcgacagct ataacaaaga
    20161 cgaccgcatg gtggcgctca acctgcgcca gcacatcagc gatcctgccc gttatcacgt
    20221 agtgatggat gaactcaacg atctggaaat gggcaaaatt ctcggtgcct gtgaactcac
    20281 cgtcggtacg cgcctgcact ccgccattat ctcaatgaac tttgccactc cggcgattgc
    20341 catcaactat gaacataaat ccgccgggat tatgcagcag ctgggactgc cagagatggc
    20401 gattgatatc cgtcatttac tggacggcag cctgcaagcg atggttgcgg ataccttagg
    20461 ccagcttccg gcgctgaacg cgcgacttaa cgaagccgtc agtcgtgagc gtcagacggg
    20521 aatgcggatg gtgcagtctg tgcttgagcg catcggggag gcgaaatgaa ggtcggcttc
    20581 ttcttattga aattcccgct gtcgtcagaa accttcgtcc tcaaccaaat cactgcgttt
    20641 attgatatgg gctttgaggt ggagattgtc gcgctgcaaa aaggcgatac agagaacacc
    20701 cacgcggcat ggacgaaata caaccttgcc gccagaaccc gctggttaca ggacgaacct
    20761 acgggcaaag tggcgaaact gcgccaccga gccagtcaga ccttacgcgg cattcatcgt
    20821 aaaaatacct ggcaggcgct caacctcaaa cgctatggcg cagagtcgcg gaacctgatt
    20881 ttgtctgcca tttgcggcca ggtcgcaaca ccgtttcatg ccgatgtgtt tatcgctcat
    20941 tttggccctg ctggggtaac cgcggcaaaa ctacgcgaac tgggtgtcat tcgcggcaaa
    21001 attgccacta tcttccacgg tattgatatc tccagtcggg aggtgctcaa ccactacact
    21061 cccgaatatc aacaactgtt tcgccgtggc gacctgatgt taccgataag cgatttgtgg
    21121 gccggaaggc tgcaaaaaat gggctgcccg agggaaaaaa tcgccgtatc gcgcatgggc
    21181 gtggacatga cgcgctttag cccgcgtccg gtgaaagcgc ctgcaacgcc gctggaaatc
    21241 atttccgtcg cacgcttaac cgagaaaaaa ggcctgcatg tggcgatcga atcctgccgt
    21301 cagttgaaag agcagggcgt ggcatttcgc tatcgcatcc tcggcattgg cccgtgggaa
    21361 cgacgcctgc gcaccctcat cgaacaatat caactggaag atgtggtgga gatgcctggc
    21421 tttaaaccga gccatgaagt gaaagcgatg ctcgacgacg cggatgtctt cctgttgcca
    21481 tcggtaacgg gcgcggatgg cgatatggaa ggtattccgg tggcgctgat ggaagcgatg
    21541 gcggtcggca ttccggtggt ttctactctg cacagcggaa taccggaatt ggtggaggcc
    21601 gataaatccg gctggctggt gcctgagaac gatgctcgcg cactggcgca acgcctggcg
    21661 gcatttagcc aactggacac cgacgaactg gctccggtta tcaaacgtgc gcgcgaaaaa
    21721 gtcgaacacg attttaacca gcaggtgatc aatcgagaac tcgccagctt gctgcaagct
    21781 ttatagagga gaacgcatgc catttaaaac actctcccga cgcaccttcc tgacggcaag
    21841 ctcggcgctt gccttcctcc ataccccttt cgctcgcgca cttcccgccc gacaaagcgt
    21901 taacattaac gactacaacc ctcacgactg gatcgcctca tttaaacaag ccttcagcga
    21961 agggcaaacg gtcgtcgtgc ctgctggatt cgtttgtgac aatatcaaca ccggcatctt
    22021 catccctccc ggcaaaacgt tacacatcct tggaagcctg cgcggcaacg gcagagggcg
    22081 atttgtctta caggacggca gccaggtgac aggggaggag ggcggcagta tgcataacat
    22141 caccctggat gtgcgaggct ctgactgcac catcaaaggg ctggcgatga gcggctttgg
    22201 tccggtaacg cagatttata tcggcggcaa aaacaaacgg gtcatgcgca acctgaccat
    22261 cgataacctg accgttagcc acgctaatta cgccatctta cggcagggat ttcataacca
    22321 gattatcggt gccaacatca ccaactgtaa gttcagcgac ttacagggcg acgccattga
    22381 atggaacgtg gcgattaacg accgtgatat tttgatctcc gaccatgtca tcgagcgcat
    22441 caactgtact aatggcaaaa tcaactgggg cattggcata ggccttgcgg ggagtactta
    22501 tgataataac tacccggaag accaggcagt gaaaaacttt gtcgtggcaa atatcacggg
    22561 atcggattgt cggcagttga tacatgttga aaatggtaaa cattttgtta ttcgtaatat
    22621 caaagcccgc aatatcacgc cggatttcag taagaaagca ggtattgata acgccacggt
    22681 cgctatttac ggttgtgaca attttgtgat tgataatatt gaaatgatta atagcgccgg
    22741 gatgttaatc ggctatgggg taattaaagg caaatatctc tcgataccgc aaaatttccg
    22801 agtgaataat attcagctgg ataacaccca tcttgcttat aaattgcgcg gcatccaaat
    22861 ctccgccggg aatgctctct cctttgtagc actgactaac attgagatga agcgtgcgtc
    22921 gctggagtta cacaacaaac cgcaacatct ttttatgcga aatatcaagg tgatgcagga
    22981 atcctcagtt ggacccgcat tgagtatgaa ctttgacatg cgcaaagacg ttcgcggagt
    23041 gtttatggcg aaaaaagaaa cactgctgtc tcttgcaaat gttcatgcgg tgaatgaaaa
    23101 aggacaaagc tccgtcgata tcgacaggat taatcaccat attgttaatg tggaaaagat
    23161 taactttaga ttgccggaac ggagggagta gatttgcgac cattcctgga aaaatggagc
    23221 catacttagg aacaatgcta ctgcaatcca caacgaagcg gcgtaacatc acaagtaatt
    23281 cagtaatcaa ttcagggtaa ttgatgctgg cgaaaaaaat cgaacaagct ataattcagc
    23341 aaccatttta caggtggatg aaataatgac gaatttaaaa gcagttattc ctgtagcggg
    23401 tcttgggatg catatgttgc ctgccactaa ggcgattccc aaagagatgc taccgatcgt
    23461 cgacaagcca atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagagat
    23521 cctcctggta actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga
    23581 attagaatct ctccttgagc agcgcgtgaa gcgtcaacta ctggcgggag tacagtccat
    23641 ttgcccgcca ggcgtgacaa ttatgaacgt gcgtcagggc gaacctttag gtttgggcca
    23701 ctccatttta tgtgcacgac ccgccattgg tgacaatcca tttgtcgtgg tgctgccaga
    23761 cgttgtgatc gacgacgcca gcgccgaccc gctgcgttac aaccttgcag ccatgattgc
    23821 gcgcttcaac gaaacgggcc gcagcctggt gctggcaaaa cgtatggcgg gtgacctctc
    23881 tgaatactct gttattcaga ccaaagaaac gctggatcgc gaaggtaaag tcagccgcat
    23941 tgttgaattt atcgaaaaac cggatcagcc gcagacgctg gactcagaca tcatggccgt
    24001 gggccgttat gtgctttctg ccgatatttg gccggaactt gaacgcactc agccaggtgc
    24061 atggggacgt atccaactga ctgatgctat tgctgaactg gcgaaaaaac agtctgttga
    24121 tgcaatgctg atgactggtg acagctacga ctgcggcaaa aaaatgggct acatgcaggc
    24181 gtttgtgaag tatgggctac gcaacctgaa agaagggacg aagttccgta aaagtattga
    24241 gatgttacta gagaagtaga tttcgagcat tagcagtcac atgttagaaa gaacaacgcc
    24301 agctaacgtc tctgcggtta ttacactaag taaaaaatgt tactccgatt ttttgtcgga
    24361 ttttccttgt ttctgccagt atttagtaag acaattgaga gttgaaatta taatgctttg
    24421 ctatggctgg tgaggcaggt tataactttg ttattcagat agtgctctgg ttgctgtaaa
    24481 gccaggggcg gtagcgtgtg tttttgagat atttatgtat tatcgttgtc atgaatacaa
    24541 gtaacaacaa agttaatgtg atctaatcaa accattagat ggtatgaata tgaagcaaaa
    24601 attaataatt attggtgctg gtggttttgc taaaactgta attgacagtt tggatcatga
    24661 aaagtatgaa atagaagggt tcattgat
//
LOCUS       DAZUGK010000051        24440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZUGK010000051 DAZUGK010000000
VERSION     DAZUGK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.051"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(98..270)
                     /locus_tag="ACHY5W_001826"
                     /pseudo
     CDS             complement(98..270)
                     /locus_tag="ACHY5W_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(481..1140)
                     /gene="rluA"
                     /locus_tag="ACHY5W_001827"
     CDS             complement(481..1140)
                     /gene="rluA"
                     /locus_tag="ACHY5W_001827"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFS4446734.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1152..4058)
                     /gene="rapA"
                     /locus_tag="ACHY5W_001828"
     CDS             complement(1152..4058)
                     /gene="rapA"
                     /locus_tag="ACHY5W_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFS4446735.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDSARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4222..6573)
                     /gene="polB"
                     /locus_tag="ACHY5W_001829"
     CDS             complement(4222..6573)
                     /gene="polB"
                     /locus_tag="ACHY5W_001829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFS4446736.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFALAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6648..7343)
                     /gene="araD"
                     /locus_tag="ACHY5W_001830"
     CDS             complement(6648..7343)
                     /gene="araD"
                     /locus_tag="ACHY5W_001830"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4446737.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7512..9014)
                     /gene="araA"
                     /locus_tag="ACHY5W_001831"
     CDS             complement(7512..9014)
                     /gene="araA"
                     /locus_tag="ACHY5W_001831"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4446738.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9025..10725)
                     /gene="araB"
                     /locus_tag="ACHY5W_001832"
     CDS             complement(9025..10725)
                     /gene="araB"
                     /locus_tag="ACHY5W_001832"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFS4446739.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11064..11942
                     /gene="araC"
                     /locus_tag="ACHY5W_001833"
     CDS             11064..11942
                     /gene="araC"
                     /locus_tag="ACHY5W_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFS4446740.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12028..12792
                     /gene="yabI"
                     /locus_tag="ACHY5W_001834"
     CDS             12028..12792
                     /gene="yabI"
                     /locus_tag="ACHY5W_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4446741.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTMLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12906..13604)
                     /gene="thiQ"
                     /locus_tag="ACHY5W_001835"
     CDS             complement(12906..13604)
                     /gene="thiQ"
                     /locus_tag="ACHY5W_001835"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFS4446742.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(13588..15198)
                     /gene="thiP"
                     /locus_tag="ACHY5W_001836"
     CDS             complement(13588..15198)
                     /gene="thiP"
                     /locus_tag="ACHY5W_001836"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFS4446743.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(15174..16157)
                     /gene="thiB"
                     /locus_tag="ACHY5W_001837"
     CDS             complement(15174..16157)
                     /gene="thiB"
                     /locus_tag="ACHY5W_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFS4446744.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESVQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16188..16287)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16529..17098
                     /gene="espY2"
                     /locus_tag="ACHY5W_001838"
     CDS             16529..17098
                     /gene="espY2"
                     /locus_tag="ACHY5W_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFS4446745.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMVNLVQKAENMNIFE
                     ELWETLRNLFRSDKHSQAAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17328..18986)
                     /gene="sgrR"
                     /locus_tag="ACHY5W_001839"
     CDS             complement(17328..18986)
                     /gene="sgrR"
                     /locus_tag="ACHY5W_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFS4446746.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNAEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19075..19206
                     /gene="sgrT"
                     /locus_tag="ACHY5W_001840"
     CDS             19075..19206
                     /gene="sgrT"
                     /locus_tag="ACHY5W_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFS4446747.1"
                     /translation="MRQFYQHYFTETAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19314..19919)
                     /gene="leuD"
                     /locus_tag="ACHY5W_001841"
     CDS             complement(19314..19919)
                     /gene="leuD"
                     /locus_tag="ACHY5W_001841"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFS4446748.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(19930..21330)
                     /gene="leuC"
                     /locus_tag="ACHY5W_001842"
     CDS             complement(19930..21330)
                     /gene="leuC"
                     /locus_tag="ACHY5W_001842"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFS4446749.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLRPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21333..22424)
                     /gene="leuB"
                     /locus_tag="ACHY5W_001843"
     CDS             complement(21333..22424)
                     /gene="leuB"
                     /locus_tag="ACHY5W_001843"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFS4446750.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPSATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22424..23995)
                     /gene="leuA"
                     /locus_tag="ACHY5W_001844"
     CDS             complement(22424..23995)
                     /gene="leuA"
                     /locus_tag="ACHY5W_001844"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFS4446751.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24088..24174)
                     /gene="leuL"
                     /locus_tag="ACHY5W_001845"
     CDS             complement(24088..24174)
                     /gene="leuL"
                     /locus_tag="ACHY5W_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFS4446752.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         5524 a         6612 c         6601 g         5703 t
ORIGIN      
        1 ctgaagaaat tgctactaaa caaagtactg ctgaagaaat tattgcaacg caattattga
       61 atgatccaga ataagctaag gttgaagggg ctggaacggc cccttcaacc ttagcagtag
      121 cgtaggatga tttcacaatt agaaagtcct gcatgatgag cgagagaaga ggctcgtgac
      181 gcaaggcgtc gtgcacgacg cggatcaccg agatgggcat cgccaaccag actgctaatt
      241 agcccatgaa taacaatcag aaaggaccat aacagacccg ataaaatgaa atagtagaga
      301 cgatcaacgg gtgaagaaaa agttcaaaaa atcgctgtgg agcagaaagg gaataaccaa
      361 atggaaagcg tagccacacg caacaactga aagcagtttg gcagaaaaaa aatccccgga
      421 ctcggggatt tatgtacaag aggcagccct taggatgagg gtataaacgt acaggaaagg
      481 ttaaaaatcc gctggcgctt taaacgtcat actattgcca tacgccggat gggtaatcgt
      541 caacatctct gcatgtagca acaaacgcgg tgccatcgct ctcgcttccg gtgatgcata
      601 aaaacgatcg ccgagaatcg gatgacccag cgccagcata tgcacacgca attgatgcga
      661 acgcccggta atcggtttta acaccactct tgccgtgtta tccgccgcat actccaccac
      721 ttcatattcc gtctgcgcag gtttacctgt ttcgtaacag actttctgtt tcgggcggtt
      781 tggccagtcg caaatcagcg gcagatctac cagaccttcc gcaggggatg gatgccccca
      841 gacgcgggcc acatactgct ttttcggctc gcgctcgcgg aactggcgtt ttaactcccg
      901 ctccgcggct ttggtcagcg ccactacaat cacgccgctg gtagccatat ccagacgatg
      961 caccgattct gcctgcggat aatcacgctg aatgcgcgtc atcacgctgt ctttgtgctc
     1021 ttccagacga cccggcacag acaacaaacc gctcggcttg ttgaccacca taatgtggtc
     1081 atcctgatac aggataacca accagggttc ctgcggtgga ttgtagtttt ccatccccat
     1141 tttcggctcc gttactgatg cgtcacaacg atcaaacgca gggcatccag acgccagcct
     1201 gcctgatcca ggctttccat tacctgctga cggttgctct caatggcagt cagttcgtcg
     1261 tcacgaatgt tcgggttcac tgcgcgcagc gcttccagac gagacagctc ggcagacagt
     1321 ttttcgtcgg cttcgttacg tgcgctatca atcaatgcac gggccgattt ctcgatctgc
     1381 gcttcaccca gctgaaggat agcgtgaaca tcctgctgca cggcattaac cagtttgctg
     1441 ccggtgtgac ggttgaccgc gttaagctgg cggttaaagg tttcaaactc cacctgggcc
     1501 gccaggttgt tgccattttt atccagcagc atacgtaccg gtgtcggtgg caggaagcgg
     1561 ttgagctgca actgcttcgg cgcctgggct tcgaccacgt aaatcagttc caccaacagc
     1621 gtacctaccg gtaacgcttt gtttttcaac agtgaaatcg tgctgctacc agtatcgcca
     1681 gaaaggatca gatccagacc gttgcggatc agcggatgct cccaggtaat aaactgcgca
     1741 tcttcacgcg ccagcgccac ttcacggtcg aaggtgatgg tgatgccatc ttccgacaag
     1801 ccagggaagt ccggcaccag catatgatcg gacggggtca gcacgatcat gttgtcgccg
     1861 cgatcgtcct gattgatacc gataatatcg aacagattca tggcgaaggc aatcaggttg
     1921 gtatcgtcat cctgctcttc aatgctttct gccagtgcct gggctttttc gccaccgttg
     1981 gaatggattt ccagcaggcg gtcacgaccc tgttccagct gtgctttcag cgcttcatgt
     2041 tgctcgcggc agtttttgat cagatcgtca aagccttcgg tttgatccgg tgaagccaga
     2101 tagttaatca gatcgttgta gacgctatcg taaatagtgc gtccggtcgg gcaggtatgc
     2161 tcgaaagcat ccagaccttc gtggtaccag cgcaccagca ccgactgagc agttttttcc
     2221 agataaggca catggatctg aatatcgtgc gcctgaccga tacgatccag acgaccaata
     2281 cgctgctcca gcagatccgg gttgaatggc aggtcaaaca tcaccatgtg gctggcgaac
     2341 tggaagttac gtccttcaga accgatttcc gagcacagca gtacctgtgc gccggtgtct
     2401 tcttcagcaa accaggcggc agcgcggtca cgctcaataa tcgacatgcc ttcgtggaac
     2461 acagctgcgc ggataccttc acgttcgcgc agtacttgct ccagttgcag cgcagtggca
     2521 gctttagcgc agatcaccag cactttctga gagcgatggc tggtcaggta gcccatcagc
     2581 cactcaacgc gcggatcgaa gttccaccag gtggcgttat caccttcaaa ttcctgataa
     2641 atacgctccg ggtagagcat atcgcgagca cgatcttccg cacttttacg tgcgcccata
     2701 atgccggaga ctttaatagc cgtctgatac tgcgtcggca gcggcagctt aatggtgtgc
     2761 agctcgcgtt tcgggaagcc tttcacaccg ttacgggtgt tacggaacag cacgcggctg
     2821 gtgccgtggc gatccatcag catcgaaacc agctcctgac gggcgctctg ggcatcttcg
     2881 ctgtcgctgt ttgctgcctg caacagcggc tcgatatcct gctcgccgat catctcgcca
     2941 agcatgttca gttcgtcatt gctcagtttg ttacctgcca gcagcatggc aacggcgtcc
     3001 gcaacaggac gataattttt ctgctcttca acgaactgcg caaaatcgtg gaaacggttc
     3061 gggtccagca gacgcagacg ggcgaagtgg ctttccatcc ccagttgttc cggggtcgcg
     3121 gtcagcagca gaacgcccgg cacgtgctct gccagttgtt caatggcctg atattcacgg
     3181 cttggcgcat cttcgctcca caccaggtga tgcgcttcat cgaccaccag caggtcccat
     3241 tcggcttcac agagatgttc caggcgctgt ttgctacgac gggcaaaatc cagcgagcaa
     3301 atcaccagct gttcggtgtc gaacgggttg taagcatcgt gctgagcttc ggcataacgc
     3361 tcatcatcaa acagcgcaaa gcgcaggttg aaacggcgca gcatttctac cagccactga
     3421 tgctgtaagg tttccgggac gataatcagc acacgttcag cagcgccaga gagcagttgc
     3481 tgatgcagga tcattccggc ttcaatggtt ttccctaaac ccacttcgtc agccagcagg
     3541 acgcgcggcg cgtggcggcg gccaacatca tgagcgatgt tgagctggtg cgggatcagg
     3601 ctggtacgct gaccgcgcag gccgctgtac ggcatacgga actgttcgct ggaatactta
     3661 cgcgcgcgat aacgcagcgc aaagcggtcc atacggtcaa tctgcccggc aaacaggcgg
     3721 tcctgcggtt tgctgaacac cagtttgcta tcaaggaaaa cttcacgcag ggctacgccg
     3781 gactcttcag tatccaggcg agtaccgata taggtcagca agccattttc ttcttttact
     3841 tcttcgactt gcatctgcca gccgtcatgg ctggtaatgg tatcaccagg gttgaacatc
     3901 acgcgggtca cgggggaatc actgcgtgcg tacagacggt tttcaccagt agatgggaaa
     3961 agtaaagtga cagttcgcgc atccaccgcg acaacggttc caagtcccaa ttcgctttct
     4021 gtatcgctga tccagcgttg accaagtgta aaaggcatat gtgttcggct ctatatcttt
     4081 aattgcaggc aataaccacc cgctaccgtg cttatgaggt agtggtgtta ttcaggtcca
     4141 ggaatggaaa gggcgctatg gtactggatg gcaaagcatt cgtcacgcat caaaatggta
     4201 tctggcgaac tctttttttg ctcaaaatag cccaagttgc ccggtcataa gtgtagcaaa
     4261 attatcctca ataaaaggga gtattccctc cgccacgggt tgtagctggc gggtcagata
     4321 gtgttcgtaa tccagtggtg aacgttggta gtccagcggc tccgggccgt tggtggtcca
     4381 tacgtactta atggtgccgc ggttctgata ttgcaagggg cgaccgcgct tttggttttc
     4441 ttcatcggca agacgagcgg cgcgtacatg aggcggaaca ttacgctgat attcgctcag
     4501 cggacggcga aggcgtttac ggtaaaccag ccgcgcatcc agttcacccg ccatcagttt
     4561 gtcgatggtt tcgcggacgt actcctgata tggctcgttg cggaagatac gcaggtaaag
     4621 ctcctgctga aactgctggg ccagcggcgt ccagtccgta cgcacggttt ccagcccttt
     4681 aaacaccatc cgctgtttgt cgccctcctg aatcagcccg gcataacgct ttttactgcc
     4741 ggtatcggct ccgcgaatgg ttggcatcag aaaacggcag aaatgggttt catactccag
     4801 ttccagtgcg ctggtcagct gttgtttttg cagcgtttcc gcccaccagg cgttaacgtg
     4861 ctgcaccagt gcacgaccga ttttcgccgc ttcttcttcc gaatgtgcgc ctttcagcca
     4921 gacaaacgtt gagtcggtat cgccgtagat cacgtcgtag ccctgcgctt caatcaacgc
     4981 tttggtttgc cgcatgatct gatgaccacg catggtgatc gacgatgcca gccgcggatc
     5041 gaagaagcgg caggcggtgg tgccgagcac gccataaaag gcattcataa tgatcttcag
     5101 cgcctgcgaa agcggcttat taccctgacg tttggcttca tcgcgcccgt gccagatgtt
     5161 agtcacaatc tccggcaggc aatgtttctc ccgtgagaac caggcatcga gaaaaccttc
     5221 ggtactgtgc tctggatcag gctgcgccat gccttccacc agcccgacgg gatcaatcag
     5281 aaaggtgcgg atgatcgacg ggtacaggct tttgtagtcc agcaccagca ccgaatcata
     5341 aagccctggc tgcgaatcca tcacatagcc gccagggctg gcgtgtggcg gcacttcgcc
     5401 gagattaggc gcgacataac cagcgcgatg catacgggga aaatagagat gaccaaacgc
     5461 tgccaccgac ccgccgtgtc gatccaccgg caggccgttc accgttgctc gttcgagtaa
     5521 aaatggcatg atttcagttt tgtggaagat ctgcgtcacc agctcgcaat ctttcaggtt
     5581 ataagttgcc agcgcaggtt tatcttcggc gaaacggcgg tcaatttcgt ccattcgatc
     5641 ccacgggtta tcgatagatt ttccttcgcc taatagctcc tgagcgacag tttccagcga
     5701 gaatgaagag aaattccaga acgcggattt cagcgcctcg ataccgtcga taattagccg
     5761 acctttagcc agggcaaaaa agacgccgtt tttaaagccg tgctcgcgcc actccagctc
     5821 actgttatcg cgccccagcc gcagcggaat acgataacgc tcggcatgtt tttgcagcat
     5881 tcgcagatcg aattgcacca cgttccaacc gatgatcaca tcaggatcgt agttggcaaa
     5941 ccaggcgtta agtttttcca gtagctgcgg gcgactggcg acgtattcca gttcaaaatc
     6001 gagcgcggag gcgtccccat tctctggccc cagcatataa acgatgcgct gcccgcaacc
     6061 ttccaggccg atgcagtaca gctcaccgtg gcgggtggtt tcaatatcaa tagaaaccca
     6121 cttgagcggc ggacgatagt cgggatgcgg tttcagacgg gcattaacga tagcgccatt
     6181 gtgcatatca ccctcgaccc acaccggtga ggtgataaac cgctccatca gatagcgttc
     6241 tggcgggcgc acatcggcct cgtagacggt aacgccacct tcacgcaggc gcttttcgta
     6301 attcatcaac tggcgatggg cgcgacagta aaggccatac accggctggc ggtgaaaatc
     6361 ctttaacgcc agcggcgtca ggcgaaagcc ttgttcaccc tgcaaaatat gctgagcgcg
     6421 gggaacctga tcggcgggaa taaacgccac ggactcttga ggcgcaagga aaacctgcaa
     6481 cggcccgttg tccgtcgcca gccagaagga gacctctgtt ccttgcgggg tatcccgcca
     6541 gtgtcgggtt aagataaaac ctgcctgcgc cacgctgaaa atccatcaaa aaaccaggct
     6601 tgattatagc ctggtttcat ttgattggct gtggttttat acagtcatta ctgcccgtaa
     6661 tatgccttcg cgccatgctt acgcagatag tgtttatcca gcagcgtttg ctgcatatcc
     6721 ggtaactgcg gcgctaactg acggcagaat atccccatat aagcgacctc ttccagcacg
     6781 atggcgttat gcaccgcatc ttcggcattt ttgccccatg caaatgggcc gtgagaatgg
     6841 accaggacgc cgggcatttg cgctgcatcg ataccctgtt tttcgaaggt ttctacgatg
     6901 acgttaccgg tttcccactc atattcaccg ttgatttctg cgtcggtcat tttgcgggtg
     6961 cagggaatgg tgccgtagaa atagtcggcg tgggtggtgc cggttgctgg aatcgactga
     7021 cccgcctgcg cccagatggt ggcgtggcgc gagtgcgtgt gcacaatgcc gccaatggac
     7081 gggaatgcct gatagagtag tcggtgagtt ggcgtatcgg aggagggctt tttcgcacct
     7141 tcaaccactt caccggtttc gatgctgacc acgaccatat cgtcagcggt catgatgctg
     7201 taatcgacgc cggaaggttt gatcacaaag acgccgcgct cgcgatcaac ggcgctgacg
     7261 ttgccccatg tgagcgtgac caggttgtgt tttggcagcg ccaggttggc ttctaatacc
     7321 tggcgtttga gatcttctaa catgttgact cctgagaggt cgtttcccct caccccgacc
     7381 ctctccccga aggggcgagg gggaaaacaa tgccggatgc gctttgctta tccggcctac
     7441 aaagtcggaa cgcgtaggcc tgataagacg cgccagcgtc gcatcaggcg ttgacagcct
     7501 gatgcggcta tttagcgacg aaatccgtaa tacacttcgt tccagcgcag tgcgtcttta
     7561 aacgcgggca ggcgggtgtc gttatcaatc accgtgattt caatgtcgtg catctcggcg
     7621 aactggcgca tatcgttgag gttcagtgca tggctgaaga cggtatggtg cgcgccacca
     7681 gcgaggatcc acgcttcgga agcagttggc agatccggtt gcgctttcca cagcgcattc
     7741 gccaccggca gtttcggcag ggagtgcggt gttttcaccg tgtcgatgca gttaaccagt
     7801 agacggtaac gatcgccgag atcaatcaag ctggcgacaa tcgctgggcc ggtttgggta
     7861 ttgaagatca ggcgggcagg atcgtcctta ccaccaatac cgagatgctg aacgtcgagg
     7921 atcggtttct cttctgcggc gatcgacggg cagacttcca gcatgtggga gccgagcacc
     7981 aggtcattgc ctttctcgaa gtgataggtg tagtcctcca taaaggaggt gccgccctgc
     8041 agaccggttg acatcacctt catgatgcga agcagggcgg cagttttcca gtcgccttcg
     8101 cccgcaaagc cgtagccctg ctgcatcaga cgctgtacgg ccagaccagg aagctgtttc
     8161 agaccgtgca aatcttcaaa ggtggtggtg aacgcgtgga agccaccttg ttccaggaaa
     8221 cgtttcatcc ccagctcaat acgcgccgct tccagcacgt tctgtcgttt ttcgccgtgg
     8281 atttgtgtgg caggcgtcat ggtgtagcag ctttcgtact catcgaccag cgcgttaaca
     8341 tcgccgtcgc tgatggagtt caccacctgc accagatcgc caaccgccca ggtattgacg
     8401 gagaaaccga acttgatctg tgcggcaact ttatcgccat cggtgaccgc cacttcacgc
     8461 atgttatcgc caaatcggca gactttcaga tgacgggtat cctgtttaga gaccgcctga
     8521 cgcatccagg agccgatacg ctcatgggct tgtttatcct gccagtgacc ggtaacgacg
     8581 gcatgttgct gacgcatacg cgcgccaatg aagccgaact cgcgaccgcc atgtgcagtc
     8641 tggttcaggt tcataaagtc catatcgata ctgtcccacg gcagcgccgc gttgaactgg
     8701 gtgtggaatt gcagcaacgg tttgttgagc atggtcaggc cgttgatcca cattttggcc
     8761 ggggagaagg tgtgcagcca caccaccaga ccagcgcaac gatcgtcgta attcgcgtcg
     8821 cggcaaatag cggtgatttc atccggcgtg gtgcccagcg gtttcaacac cagtttgcag
     8881 ggcagtttcg cttccgtatt tagcgcatta acaacgtgct cggcatgttg ggtgacctga
     8941 cgcagggttt ccgggccata cagatgctgg ctgccaatta caaaccacac ttcataatta
     9001 tcaaaaatcg tcattatcgt gtccttatag agtcggaacg gcctgggcag cctgtgccgg
     9061 ggcggaagtt ggaagatagt gttgttcggc gctcatcgcc cattgctgat agcggcgata
     9121 aagctgttca aagcgttgtg cctgctcgct gcacggttgc agggttttct ctaccgcact
     9181 ggccattttt tgctgggctg atgggatgtc tgcgtgcact ttcgcggcga cggcagcaaa
     9241 aatcgccgca ccgagcgcac agcactggtc agaggcaaca atttgcagcg ggcgattcag
     9301 cacgtcgcag caggcctgca taatgacctg gtttttccgc gcgatgccgc ccagcgccat
     9361 cacgttatta acggcgatcc cctgatcggt aaagcactcc atgattgcgc gtgcgccaaa
     9421 ggcggtggca gcaatcaaac cgccgaacag cagcggagca tcggtagcga ggttaagatc
     9481 ggtaatcacc cctttcaggc gttggttagc gttcggtgtg cggcggccgt taaaccagtc
     9541 gagcaccacc ggcaggtgat ccagagacgg atttttggcc catgcttcgg tcagcgccgg
     9601 aagcagttgt ttctggctgg cgttgatttg ctcttttagt tccggatgct gggcggcaag
     9661 ctgttccagc ggccagccga gtacgcgacc gaaccaggcg tagatatcac caaacgccga
     9721 ttggcctgct tccagaccga taaatccagg caccacgctg ccatcaacct gaccgcaaat
     9781 acctttcact gcccgctcgc caacgctctg tttgtcggca atcagaatgt cgcaggtgga
     9841 agtaccgata acttttacca gtgcgttagg ctgtgcgcct gcgccaactg cgcccatatg
     9901 gcagtcaaac gcgccgccgg aaatcaccac gctttcaggc aggccgagac gctgcgccca
     9961 ttccgggcat aaggtgccca ccggaatatc ggcagtccag gtgtcagtga acagcgggga
    10021 aggcaaatgg cgattgagga tcgggtccag ctcatcaaag aaactggctg gcggcaggcc
    10081 gccccagctt tcgtgccaca gagatttatg cccggcgctg caacgtccgc gacgaatatc
    10141 ctgcgggcgg gtggtaccgg aaagcagagc tggcacccag tcgcacagct caatccacga
    10201 tgcggcagat tgcgccacgg cgctgtcctg gcgagtcaca tgcaggattt ttgcccagaa
    10261 ccattcgctg gaataaatac cgccaatgta gcgggagtag tcaacgttgc ccggcgcgtg
    10321 gcacaaacgg gtaatctctt ccgcttcttc aaccgcagtg tggtctttcc acaatacgaa
    10381 catcgcgttc gggttttcgg caaactccgg gcgcagcgcc agcacgttac cgtcggcatc
    10441 aatcggtgcg ggcgtcgagc cggtactgtc aacgccaatc ccgaccacag ctgcgcgctg
    10501 ttcgacgcta agctctgcaa gcacggtttt cagtgccgct tccattgact caatgtagtc
    10561 acgcggatga tgacggaact ggttattcgg ggcatcacaa aattgccctt tttgccaacg
    10621 gggataccac tctacgctgg tggcgatctc ttcaccgctg gcgcagtcca ccgccaaagc
    10681 tcgcacagaa tcactgccaa aatcgaggcc aattgcaatc gccatcgttt cactccatcc
    10741 aaaaaaacgg gtatggagaa acagtagaga gttgcgaaaa aaagcgtcag gtaggatccg
    10801 ctaatcttat ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaattatcaa
    10861 tgtggacttt tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg
    10921 tcccgctttg ttacagaatg cttttaataa gcggggttac cggttgggtt agcgagaaga
    10981 gccagtaaaa gacgcagtga cggcaatatc tgatgcaata tggacaattg gtttcttctc
    11041 tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat
    11101 actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg
    11161 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
    11221 gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt gatattttgc
    11281 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc
    11341 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa
    11401 tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc
    11461 tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga
    11521 taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc
    11581 caccgatgga taatcgtgta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca
    11641 gcaattttga tatcgccagc gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac
    11701 atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgcatta
    11761 gtcaggcgaa gctgcttttg agcaccaccc ggatgcctat cgctaccgtc ggtcgcaatg
    11821 tcggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc
    11881 cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat
    11941 aattggtaac gaatcagaca attgacggct tgacggagta gcatagggtt tgcagaatcc
    12001 ctgcttcgtc catttgacag gcacattatg caagcattgc tggaacactt tattacccaa
    12061 tccaccgtgt attcattgat ggcggtggtg ttggtggcct ttctggagtc gctggcgctg
    12121 gtcggtttga ttctacccgg tacgatgctg atggcggggc tgggagcgct gattggcagc
    12181 ggcgagttaa gtttctggca cgcctggctg gcagggatcg tcggttgcct gctgggcgac
    12241 tggatctcct tctggctggg ctggcgtttt aaaaagccat tgcatcgctg gtcatttctg
    12301 aagaagaaca aagcattact cgataaaact gaacatgcgt tgcatcaaca cagcatgttc
    12361 accattctgg tcggtcgttt tgttggcccg acgcgtccgc tggtgccaat ggtggcggga
    12421 atgctggatc tgccggtggc taaatttatt acgccgaata ttatcggctg cctgctgtgg
    12481 ccgccgtttt acttcctgcc agggattctg gcgggcgcgg cgatcgatat tcctgccgga
    12541 atgcagagtg gcgagtttaa atggttgctg ctggcgacgg cggtattttt gtgggttggt
    12601 ggctggctgt gctggcggtt atggcgcagc ggcaaagcaa ccgatcgttt gagtcattat
    12661 ttgtcccgcg gtcgtttgtt gtggctgacg ccgttgattt ctgccatcgg cgtggtggcg
    12721 ctggtggtgt taattcgcca tccgttaatg ccggtgtata tcgatatttt gcgtaaagtg
    12781 gttggagttt agggcatagt cttgtgtggg ttgcctgatg cgacgcttgc cgcgtcttat
    12841 caggcctaca aaacgcacta cccgtaggtc ggataaggcg ttcacgccgc atccgacagt
    12901 gcatattaac ctgtaattcc caacagcgcc gaagcactcg ccttaccgct caacaattct
    12961 tcggtcttac cctgccaggc gatgcgcccg tcggcgacta ctaccgagcg cgtggcaatc
    13021 cgcgccgcat cttccacact gtgcgagacc atcaatagcg tcattttttg ctgctggcag
    13081 ctcgtgctca ccagcgtcaa catctcctgg cgtagcgccg gatcgagcgc agagaacggt
    13141 tcatcgagca ataaaatcgg ctgctcgcgt accagacaac gcgctaacgc cacgcgctgt
    13201 cgctgaccgc cggaaagctc gcccggtaac cgcgccatta aattatcaat ccccatctga
    13261 cgggcaatcg cgtgcatttt ctcctgctgt gccgcgttca gtttcaatcc cggatttagc
    13321 cccagcccga tgttctgcgc gaccgtcagg tggctgaaca ggttgttctc ctgaaacagc
    13381 atcgacaccg gacggcgtga cggcggcgtc gtggtgtgat caacgccatc gatagtcagc
    13441 gaaccgctgg ctggcgtcag aaaaccggcg atcaaattca gcagggtact tttacccgcg
    13501 ccgcttggcc cgaggatcgc cacctgctcg ccgcgttcca ccgttaagct aaaacgcatc
    13561 ggcaaatgtt ggtaaagcca ggtgatatca gtcagtttta acatttcgcc ccggtatttt
    13621 ttcaatcacg gtaaacagca gaaaacagag tagtagcaga attaacgcgg tgaccgcgcc
    13681 gtcctggctg cgataggagc caatttgctg gtagagataa aacggtaggg tgcggaaatc
    13741 atcgttaccg aacaacgcca ccacgccaaa atcaccaatc gacagcacac aggcaaaggc
    13801 cagcgcctgc gccagcgggc gtttcagggc gcgcagctcc accactttta agcgtgacca
    13861 gccttcaatc cccagcgact gacataacat gctgtagcgg gcggtgatat cgcgcatcgg
    13921 gttttccagc actttcaggg cgtaggggat cgccattaac gcattggtga aaatcacaat
    13981 gccgtcagca gattgtggca ggccgatagt gttgttgagc agtaaaaaga agcccgttgc
    14041 cagcacaatc cccggcatag cgaggatcaa catgccgctc atctccagcg cctggcccgc
    14101 cagcattttt tgccgcgccc gaagttcgcg actgctccat agcagcatca tggtcagcac
    14161 tacgcacaat acacctgccg ccagcgcaat acgcaacgag gtccacagcg cctgccacag
    14221 caccggttgt gccagcactt ccggcaactg gcgatttacc ccatcgacga tcaccgccag
    14281 caacggtggc agcaacagca gcagcgccag cactattaac accgtgtcgc aaatgcggct
    14341 atgcagacga tcgtccgggt cgcgccagcc ttgcagcagc gtggtgccgg gcgcaatggc
    14401 cttactcagt cgctgactca acagcaccag cccaaggcaa cacaccatct ggatcagcgc
    14461 cagcatcgcc gcgcgggcag gatcgtagtc gtaactcagc gcctgataga ttgccagctc
    14521 gatagtggtc gcctgcggac cgccgcccag cgacagtacg gtggcgaagc tggcgaaaca
    14581 gagcataaag ataagcgcgg taaccggcgg gatttgtcgc cgtaaccacg gccattcgac
    14641 gaagcggaaa aaatgccagc cgcgcatccc aagctgggcg gcaagctgac gttgttcgcc
    14701 gggaatgttt tccagtgcct ggagtaataa gcggctcgcc atcggcagat taaaaaacac
    14761 atgggccagc aaaatgcctt gcaggccgta gggcgaaaag gtccactcca gaccgagcga
    14821 ttggcagagc gatgccagcc agccctggcg accatagacg ctaagaatgc cgaaaacggc
    14881 gaccagcacc gggaggatca aggtcattgc gcacagacgc aacagcgcca gccgacccgg
    14941 aaagcgcctg cgatagagcg cgcgggcgag gaatatcgcg ggtacgacag agagtagcgc
    15001 cgagagaaac gcctgccaga aggagaagcg caccacatgc cacaggtagc tgtcctgcca
    15061 gactgccgac cagtcaccct gcggcgcgtt ccaccacagg gcgagaaacg ccgccagcgc
    15121 aaccgctacc accagcgtgg cggcgcttac acctggaatt aaccagccgg gaattaacgg
    15181 ctgacggcgc gttgccattc gctaatccat gcctgacgtt gtgccgccac ttcggctggc
    15241 gtgaactcca gcgtggttgc gggtttggtc aatttttcaa aaccggcagg cagcgtgacg
    15301 tttgccaccg gatacatcca gttgccggtt gggatcgcat tctggaaagc cggagaaacc
    15361 ataaactgga ggaatttttg cgccagctcc ggctgcttgc tggcagcggt gcgagcggcg
    15421 acttccactt gcagatagtg accttcgctg aagttcgcgg cggcgtagtt atctttcttc
    15481 tcttcgagaa tgtgataagc cggagaggtg gtgtaactca gtaccagatc gctttcacct
    15541 tttaaaaaca ggccgtaggc ttcgctccag cctttggtga ccgtgaccgt tttcttcgcc
    15601 agtttctgcc aggcttgtgg ggcgtcatcg ccatagactt tttgcatcca tagcaacaga
    15661 cccagccccg gtgtactggt gcgcggatcc tgataaatca ctcgccagtt ttgaacgctc
    15721 tcaaccagtt ctttcaggct ttgtggcggg tttttcagtt tgttcttgtc ataaacgaag
    15781 gcgaagtagc cataatcaaa cggtacgaaa gtgtcattat tccagccgcc gggaacgtta
    15841 acggcatccg ctgccacacc gcttttggca aacagtccgg ttttactggc ggcgtctaac
    15901 aggttgttat ccagccccag caccacatcg gctttactgt ttttgccttc catccgtaga
    15961 cggttgagaa gcgaaacgcc atcttccagc gccaccagtt tcagttcgca attacagtcg
    16021 gcttcaaagg cttttttaac caccggacca ggcccccagt cggcggcgaa ggaatcgtag
    16081 gtataaacag tcagaacggg tttagcgaaa acgggcgctg tgcacagcaa cagcagggga
    16141 agacattttt ttaacacttt gcacctcaaa aaagagtggc aaaggacttg agaaggagcc
    16201 tcaaatccct tcgccggcgt tatccggatc aggttcgacg ggtattttct cagcgcacgc
    16261 gtccgcgtgg caccccgttg agaacggcgt tagtgtagtg attttgttat caaccagcaa
    16321 tccatagaga aggcgtatta aaaaagccaa tattcgttca gctatcaaga gcgtatttgc
    16381 atagctggag agcgttgatc cgaaagaatc cccttagcac agtcaaaatc ggtaacgcaa
    16441 cggcttatta cgtgaatgag cgatttttga cagtattctt attcaagacg cttattctga
    16501 gttttttact cagagtggag ctatcagcat gaaagtaaga aacccagaac agattagtat
    16561 ccctgctagt aataccacta aagatccagg ccttactaat tctcagatta tcaggatggt
    16621 taacttggtc caaaaggccg aaaatatgaa tatctttgaa gagctatggg agacgttacg
    16681 caatctcttc cggtcagata aacattccca ggctgcggca aggcaaattc tgaaggatgc
    16741 attttatttc cagaatagtg atgactactc aaaatacttt accggggctg tcgacgggaa
    16801 ggcgcgtgat cagttaactc actggctgat aaaatttaat gaactcaaag aatacgcaaa
    16861 agatccggaa aatatggccg cgaaggcctc actctcgcca gaaggtacgt tatgcgtcag
    16921 tttttttatt ggcgatgagg cgatatttac tctggagttg cagctgaaaa agagcaccag
    16981 gaccgggggc attgatctga gtaatgctta ctttaacggg gttgtcattt gtggtattga
    17041 ctgtctcgag gttgacctga gcaatgctga aacgaacaat agcagatggt atgactgacc
    17101 tgcgcgaggt taacctgaaa aagctgacct ttccggccag aacacggcta gactgcgccg
    17161 ttattgatta actcagaata tgaaagaggg gctggaatgc cccttcttta atcttatttg
    17221 cgactctaat cagacgatgc ataaaacaga gaaaatataa aagtaatgta ttttattcat
    17281 tccgtctgaa tataaaaaac gcgctcatga ccgccaaaag gcgcaagcta ctctggatcc
    17341 ggcggcgcaa accacgctga tttaaaatcg aaccagccga gggtgttcat gcgtaggccg
    17401 cgcatactgc gttgcccctg aatgatcagc cagtggtgga taagtggcac cattgcttta
    17461 ctggcgacca gttgctggca ccagttcgcc agattcatct cggcattgcg ccagcgggcg
    17521 gcgtcggctt gccagtcgat cggaatacag tgttggagca gcggcacctc gcacaggtgc
    17581 gcgaacagcg aaaaatccag cggtagcgta aagttggcgc tgttaagcca gatatcgctc
    17641 tcgatctctc cttcatgcca ctgatcgtag ctgatctctt tgatttccag tgtgacctgg
    17701 tggcttgcca gaatctgctg catgatcccg gcaatcaccc gatgctcact gtgatcctga
    17761 taaaaggtca gggtgaggct ttccagacca gccggttttt cgctctttat ggtgcgggca
    17821 tgatgccaac gggggagcag tccataagcc gggaaccaca gttgctggta ctgttcctca
    17881 gcgaaataga ccagattagt tggagaaagc acatagctta cccagtccct gacttgctga
    17941 ttcgccccgc gatgggtgcg gctatcgaac agtaaatagt agcaaccttc ctccaggcgg
    18001 ctttcaatct ctttttcctc gccctgtgga ccttttagca tcagccctcc ggctggctcg
    18061 tcggcaattt ccggcagaac ccagacgtta acttcatcga ttaatgcccg gtaaccgaag
    18121 aagtcatcga atgcctgaat tttcagttga ttggtgctgt tgcgaatcac cgcatacgga
    18181 ccggtgccga tgggatggct ggcaaagtta ctgagggttt cccattcgcg cggcaggatc
    18241 atcgccggaa cttgtcccag cagtaacggc aaccagcgat ccggctgcgt gaggtggata
    18301 tccagcgtcc agggcgtcgg cgacacaatg tcagcaatat gcgaatagag cggcagcgta
    18361 ttgattcgtt ttaaagaggc gatcacgtcg tccatttcca gttcacgacc atgatgaaaa
    18421 tggactcctg gacgcaaaaa gaaacgccag tgaagcggtg atatttgctg ccagtggtgg
    18481 gcgatgtctg cttccagttc cccattttcc tcatttatgc gcgttagcga actgaagatt
    18541 tgccgggcga tatgggtttc ggaacggcgc aatgcgctgc caggtagcag attacgcaac
    18601 ggacgatagt agagcacgcg caggatgtgc cgcccctggc ggaagctgcg gcccagatga
    18661 gaaaccagca tttgcctcac agtcgctttg tcgccaacta actgcactag ttggtcgata
    18721 cggtcctgct ccagcaggtc ttccgcccgc tgttgctgaa gcgccagccc ggtatagagg
    18781 aatgtcagac gcgagcgttt accacgcccg acttccgctt cccacgtcag ccagccgcga
    18841 tcctgcatgg tgttgagcag ggtgcgcata tgacgacgcg agcagctcaa taacgctgcc
    18901 agttcgttga gcgttgtgtc ctgcgattta ccctcgcagc attgccacag gcggatgaac
    18961 tgttgttgca gacgagcaga tggcataaaa ggggaactcc tgcgcaaaag acagcaattt
    19021 tattttccct atattaagtc aataattcct aacgatgaag caaggaggtg ccccatgcgt
    19081 cagttttatc agcactattt taccgagaca gcgaagttgt gctggttgcg ttggttaagc
    19141 gtcccacaac gattaaccat gcttgaagaa ctgatgcagt gggaggggag tcattctgac
    19201 tactgatttg cggacatcat gtgtgactga gtattggtgt taatcgccac gccagcagtg
    19261 attatctgct ggcttttttc tttttggcga ttgagtaccg gacggtgtac aaactaacgc
    19321 ataaacgcgg gttgcttcgc ttcataagcc gcaatggcgt cgtcgtgctg caaggtaagc
    19381 ccaatgctgt ccagaccgtt catcatgcag tggcggcgga aggcatcaat ggtaaagcga
    19441 taggttttct cacccgcttt cacctcttgc gcttccaggt ctacatcaaa atgaatcccc
    19501 ggattggctt tcaccagcgc aaacagctca tccacttctg cgtcgcttaa tttcaccggc
    19561 agcagctggt tgttaaagct attgccgtag aagatgtcag caaaactcgg cgcaatcacc
    19621 accttaaaac cgtagtcggt caatgcccag ggcgcgtgct cacgcgagga gccacagccg
    19681 aagttttccc gcgccagcaa aatggaagcc ccctgatact gcgggaagtt cagcacgaag
    19741 tccgggtttg gctgttggcc tttttcatcc agaaaacgcc agtcgttaaa cagatgcgcg
    19801 ccaaaacccg tacgggtcac tttctgcaaa aactgtttcg ggatgattgc atcggtatcg
    19861 acattggcgg catccagcgg aaccaccagg cctgtgtgtt tgataaattt ctctgccatg
    19921 gtgtgctcct tatttaatgt tgcgaatgtc ggcgaaatgg ccggtcacag ccgcggcggc
    19981 agccattgcc gggctgacca gatgcgtgcg cccgccgcgc ccctgacggc cttcaaagtt
    20041 acggttgctg gtggaggcac aacgttcgcc cggattcaga cggtcgttgt tcatcgccag
    20101 acacattgag cagccaggca agcgccattc aaaaccggct tcaataaaga ttttgtccag
    20161 accttccgct tccgcctggg cttttaccgg gccagagccg ggaaccacca gtgcctgtac
    20221 gcctggcgcg acttttcgcc ctttggcgat ttcagccgcc gcgcgtaaat cttcaatgcg
    20281 cgagttggtg caggagccga taaacacttt gtcgatagcc acttctgtca gtggaatacc
    20341 cggtctcaac cccatatagg ccagcgcttt ttctgctgac gcgcgttcaa ccggatcggc
    20401 aaacgaagcc ggatcgggaa tattgtcgtt cacggaaatc acctggcctg gattggtgcc
    20461 ccaggtgacc tgcggcgaaa tctcttctgc ttgcagagtg acaacggtat cgaaagttgc
    20521 gccttcgtcg gtttgcaggg ttttccagta ggcaacggcg tcgtcgaaat ctttgccttt
    20581 cggcgcatgc agacgacctt tgacatagtt aaaggtggtt tcgtccggtg caaccagacc
    20641 ggctttcgcg cccatttcga ttgccatatt gcacagggtc atccgacctt ccatgcttaa
    20701 atcacggatt gcttcgccgc aaaactccac cacatgcccg gtgccgcctg cgctgccagt
    20761 tttaccgata attgccaaca cgatatcttt tgcggtaatg cccggcgcgg ctttgccctg
    20821 gacttcaatt ttcatggtct tcgcacggcc ctgtttcagg gtttgcgttg ccagtacgtg
    20881 ttcaacttcg gaagtgccga taccaaaggc cagtgcgcca aacgcgccgt gagtggcggt
    20941 atgcgagtcg ccgcagacaa tggtcatccc cggcaaggtc acgccctgtt ccggccccat
    21001 tacgtggacg atcccctgat acgggtgatt caggtcatac agctcgacgc caaattcttt
    21061 gcagtttttg atcagctcct gcatctggat gcgcgccatt tcaccacagg cattaatgtc
    21121 tttggtctgg gtagagacgt tgtgatccat ggtggcgaag gttttgcccg gctgacgtac
    21181 cgggcgaccg tgggcgcgca gaccatcgaa cgcctgcggt gaggtcactt catgcaccag
    21241 gtggcggtcg atatataaca gcggggtttc gttttcggct tcgtatacta cgtgagcgtc
    21301 gaataatttt tcgtataacg tcttagccat gattacaccc cttctgccac atagcgggca
    21361 atgatatcgc ccatttcatc ggtactaacg gcggcagcgc cacgggctaa atccccggtg
    21421 cgaatgcctt cttctaatgc gcggttaatg gcgcgttcaa tggcggaagc cgcatcatcg
    21481 gcatccaggc tgtaacgcag cagcagtgcc agcgacagga tttgtgcaat cgggttggcg
    21541 atgtttttgc ctgcgatatc tggtgccgag ccgcccgccg gttcatacag tccaaaacct
    21601 tgctcgttca ggctggcgga aggcaacatc cccatcgagc cagtgatcat tgcgcactcg
    21661 tcagacagaa tgtcgccaaa caggttggag cacagcagaa cgtcaaactg tgatggatct
    21721 ttaatcagct gcatggtggc gttgtcgatg tacatatgcg ccagttcgac atccgggtat
    21781 tccgtggcga tctcgttgac gatttcacgc cataaaatag aggattgcag cacgttagct
    21841 ttgtcgattg aggtgacttt gtggcgacgc ttgcgagcag attcaaacgc gatgcgggcg
    21901 atacgttcaa tctcaaaacg gtgatacacc tcggtatcaa acgctttttc atattgtccg
    21961 ctaccttcgc ggccttttgg ctgaccgaaa tagatgccgc cggtcagttc gcgcacgcac
    22021 aggatgtcga agccgttagc ggcaatgtca gcacgcagcg gacagaatgc ttccagcccc
    22081 tgatacagtt ttgccggacg caggttgctg aataatttga agtgcttacg caaaggcaac
    22141 agtgcgccgc gttctggttg ctggtccggt ggtaaatgtt cccatttcgg gccgcctacc
    22201 gagccaaaca gcacggcatc ggcttgctca caaccttcaa ccgtcgcaga cggcagtggt
    22261 tgcccgtggt tatcaatggc tgcgccgcct acatcgtaat ggctggtagt gatgcgcatc
    22321 gcaaagcggt tgcgcacggc atccagcact ttcagcgcct gggtcatcac ttccggacca
    22381 ataccgtccc ccggcaatac ggcaatatgg taattcttcg acatcacacg gtttccttgt
    22441 tgttttcgtt gtgttgagct ttgcgttgca actctttttc gacttctgtg gcacgccaga
    22501 tattgttaag tacgtgcacc atggctttgg cggaggactc gacaatatcg gtggccaggc
    22561 cgacgccgtg gaagcggcga ccgttgtagt tggcgacaat atccacctga cccagcgcat
    22621 ctttaccgtg acctttagcg gtcaggctgt atttcaccag ttcgacgtta tagtcagtga
    22681 tgcggtttat cgcctgatag acggcatcga ccggaccgtt accgttggcg gcttctgctt
    22741 tgacttcttc gccgcaggcc agtttgacgg cggcggtggc gatatcgtta gagccagact
    22801 gcacgctgaa gtaatccaga cggaaatgct ccggctcttc ttgctgctta ccgatgaagg
    22861 ccagcgcctc cagatcgtaa tcaaacacct gacctttttt gtccgccagc ttcaggaagg
    22921 cgtcgtacaa attgtctaaa ttatattcac tttctttata ccccatctca tccatgcgat
    22981 gtttcaccgc cgcacgcccc gaacgagagg tcagattcag ctggatttgg ttcagaccaa
    23041 tagattctgg tgtcatgatt tcgtagtttt cgcggttttt cagcacgcca tcctggtgga
    23101 taccggagga gtgtgcgaat gcgccgctgc caacaatggc tttgtttgcc gggatcggca
    23161 tattacaaat ctggctaact aactggctgg tgcgccatat ctcctggtga ttaatggcgg
    23221 tgtggacgtt gagaatatcc ttacgaactt taatcgccat gatgacttct tccagggaac
    23281 agttaccggc acgctcgccg atcccgttca ttgcaccttc cacctggcgt gcaccggcat
    23341 gtacagccgc cagtgagttg ccgaccgcca ggcccaaatc gtcgtgggta tgtacggaaa
    23401 taatggcttt gtcgatgtta ggcacgcgtt catacaggcc gctgatgatt ccggcgaact
    23461 caaacggcat ggtgtagccc acggtgtccg gaatgttgat ggtggtggca ccggcattaa
    23521 tcgccgcttc gaccactcgc gccagatcgg caatgggtgt acgcccggca tcttcgcaag
    23581 aaaattcaac atcatcggtg taattacggg cgcgtttcac catatagata gcgcgttcga
    23641 tcacctcgtc cagcgtgctg cgcagcttcg tggcgatgtg cattggcgaa gtggcaataa
    23701 aggtatgaat acggaaggct tcggcgactt tcagggattc ggccgccacg tcgatatctt
    23761 tttccacgca gcgagctaac gcgcatacgc ggctgttttt aacctggcga gcgatggttt
    23821 gcactgattc aaaatcaccc ggcgaagaga cgggaaaccc gacttccatc acgtcaacac
    23881 ccatacgctc aagggccagc gcaatttgca gtttttcttt cacactcaag cttgcctgta
    23941 acgcctgttc accgtcgcgc aatgtggtat cgaaaataat gacttgctgg ctcatggttt
    24001 gggtccttgt ctcttttaga gcgcctcgct tcgggcataa aaaaacccgc gcaatggcgc
    24061 gggttttttg tttgactgcg tgctggctta atgctggatg ccgctcactc gtctaccgcg
    24121 caaagaagat gcgtttagta gtagtagacc gataaagcga acgatgtgag tcattaaatc
    24181 agctccagat gaatgcgata tgcttttaga gttactggat acaaaaacgg atgtcaaccc
    24241 tgacgcaata aaaacgtccc gccagcatga gttctgcatc cgtaaaatta gctaattgtg
    24301 ctgctgtggt taaaacaagc gatattaatt tctgcttaac taccgacgct tttcatcggt
    24361 tgacatattt cagaataaat ttttgcatct aatccacgag gaaaaagggg acaaaatgca
    24421 cgcgttgcaa aacctatcct
//
LOCUS       DAZUGK010000052        24153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZUGK010000052 DAZUGK010000000
VERSION     DAZUGK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.052"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(155..1018)
                     /gene="potB"
                     /locus_tag="ACHY5W_001846"
     CDS             complement(155..1018)
                     /gene="potB"
                     /locus_tag="ACHY5W_001846"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFS4446753.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(1002..2138)
                     /gene="potA"
                     /locus_tag="ACHY5W_001847"
     CDS             complement(1002..2138)
                     /gene="potA"
                     /locus_tag="ACHY5W_001847"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFS4446754.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2388..3614
                     /gene="pepT"
                     /locus_tag="ACHY5W_001848"
     CDS             2388..3614
                     /gene="pepT"
                     /locus_tag="ACHY5W_001848"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4446755.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3663..4784)
                     /gene="roxA"
                     /locus_tag="ACHY5W_001849"
                     /gene_synonym="ycfD"
     CDS             complement(3663..4784)
                     /gene="roxA"
                     /locus_tag="ACHY5W_001849"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFS4446756.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5033..6262
                     /locus_tag="ACHY5W_001850"
     CDS             5033..6262
                     /locus_tag="ACHY5W_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4446757.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6627..6815
                     /locus_tag="ACHY5W_001851"
     CDS             6627..6815
                     /locus_tag="ACHY5W_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4446758.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6873..7427
                     /locus_tag="ACHY5W_001852"
                     /pseudo
     CDS             6873..7427
                     /locus_tag="ACHY5W_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7620..7817
                     /locus_tag="ACHY5W_001853"
     CDS             7620..7817
                     /locus_tag="ACHY5W_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4446759.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7810..8022
                     /locus_tag="ACHY5W_001854"
     CDS             7810..8022
                     /locus_tag="ACHY5W_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446760.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            8012..8476
                     /locus_tag="ACHY5W_001855"
     CDS             8012..8476
                     /locus_tag="ACHY5W_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446761.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8469..8702
                     /locus_tag="ACHY5W_001856"
     CDS             8469..8702
                     /locus_tag="ACHY5W_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446762.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8708..9007
                     /locus_tag="ACHY5W_001857"
     CDS             8708..9007
                     /locus_tag="ACHY5W_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4446763.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            9004..10404
                     /locus_tag="ACHY5W_001858"
     CDS             9004..10404
                     /locus_tag="ACHY5W_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFS4446764.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10605..10856
                     /locus_tag="ACHY5W_001859"
     CDS             10605..10856
                     /locus_tag="ACHY5W_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446765.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10853..11263
                     /locus_tag="ACHY5W_001860"
     CDS             10853..11263
                     /locus_tag="ACHY5W_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4446766.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11274..11546
                     /locus_tag="ACHY5W_001861"
     CDS             11274..11546
                     /locus_tag="ACHY5W_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4446767.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11503..11631
                     /locus_tag="ACHY5W_001862"
     CDS             11503..11631
                     /locus_tag="ACHY5W_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4446768.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11673..11897
                     /locus_tag="ACHY5W_001863"
     CDS             11673..11897
                     /locus_tag="ACHY5W_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446769.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12149..12355
                     /locus_tag="ACHY5W_001864"
     CDS             12149..12355
                     /locus_tag="ACHY5W_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446770.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            12355..13410
                     /locus_tag="ACHY5W_001865"
     CDS             12355..13410
                     /locus_tag="ACHY5W_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4446771.1"
                     /translation="MAGKATALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFNT
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNLIEGEGIERYE
                     IDWKIPESNIIEQADGKKWSELDKETHDPIYDIELYADQGNCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALVVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13423..13758
                     /locus_tag="ACHY5W_001866"
     CDS             13423..13758
                     /locus_tag="ACHY5W_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4446772.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLAENGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESADTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13771..14184
                     /locus_tag="ACHY5W_001867"
     CDS             13771..14184
                     /locus_tag="ACHY5W_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFS4446773.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNSLEEKD
                     KPRADAIMALHESDDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            14390..14932
                     /locus_tag="ACHY5W_001868"
     CDS             14390..14932
                     /locus_tag="ACHY5W_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4446774.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15189..15470
                     /locus_tag="ACHY5W_001869"
     CDS             15189..15470
                     /locus_tag="ACHY5W_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446775.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(16072..17532)
                     /gene="phoQ"
                     /locus_tag="ACHY5W_001870"
     CDS             complement(16072..17532)
                     /gene="phoQ"
                     /locus_tag="ACHY5W_001870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFS4446776.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(17532..18203)
                     /gene="phoP"
                     /locus_tag="ACHY5W_001871"
     CDS             complement(17532..18203)
                     /gene="phoP"
                     /locus_tag="ACHY5W_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFS4446777.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18372..19742)
                     /gene="purB"
                     /locus_tag="ACHY5W_001872"
     CDS             complement(18372..19742)
                     /gene="purB"
                     /locus_tag="ACHY5W_001872"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4446778.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(19746..20387)
                     /gene="hflD"
                     /locus_tag="ACHY5W_001873"
     CDS             complement(19746..20387)
                     /gene="hflD"
                     /locus_tag="ACHY5W_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFS4446779.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20423..21529)
                     /gene="mnmA"
                     /locus_tag="ACHY5W_001874"
                     /gene_synonym="trmU"
     CDS             complement(20423..21529)
                     /gene="mnmA"
                     /locus_tag="ACHY5W_001874"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4446780.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(21583..22044)
                     /gene="nudJ"
                     /locus_tag="ACHY5W_001875"
     CDS             complement(21583..22044)
                     /gene="nudJ"
                     /locus_tag="ACHY5W_001875"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFS4446781.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22055..22708)
                     /gene="rluE"
                     /locus_tag="ACHY5W_001876"
     CDS             complement(22055..22708)
                     /gene="rluE"
                     /locus_tag="ACHY5W_001876"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFS4446782.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREVTD"
     gene            22880..24130
                     /gene="icd"
                     /locus_tag="ACHY5W_001877"
     CDS             22880..24130
                     /gene="icd"
                     /locus_tag="ACHY5W_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFS4446783.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6371 a         5805 c         6270 g         5707 t
ORIGIN      
        1 ttgttgtttt tttatagcga tttgctaaaa gggagaagaa aaaacgttct ccaaaattag
       61 attgcaactg tttgatttta tggatagagg catgctgttt actagacagt aaaaagcatg
      121 ccaaatttcc tattaacatt ttgaatataa agcactaatt atctatttct gttgccttct
      181 tattcagcag acgagaagcg cgccagtaaa ccagcaacat caggcccatt acgatagtca
      241 gcgtaatgct ggtggctgca ccaaacggcc agtcacgaat attaaggaac tggaccttga
      301 tgacgttacc gatcagcagg tttttcgcac cgcccatcag gtcggacaca tagaacaggc
      361 ccatcgctgg cagcatcacc agcagacatc cggcaataat ccccggcatc gtcagtggaa
      421 taatgatacg gataaaagtc tgtaacttgc tggcaccgag atcgcgcgcc gcctcaagca
      481 acggcttatc cagtttttcg atactggagt acagcggcat caccataaac ggcagcagaa
      541 tgtaaaccag accgataatc accgcactgg gcgtgaacat gatacggatt ggtgtgtcga
      601 taacgcccag ccagagcaaa aactcgttga ggtagccttt ggtgctgagg aaaattttca
      661 gcccgtagat gcgaatcagt gagttggtcc agaagggaac aatcagcaga aacagcagca
      721 gcggacgcac cttgtgtggc agcttcgcca gaaaccaggc aaacggatag cccagcacca
      781 ggcaggcgag ggtggcgatc agcgccatat tcagcgagtg caatagcact tcaaaataga
      841 gcggatcgag cagacgtgtg tagttatcca gcgtaaagac cattttgacg aaactggcgt
      901 cgtcgcgggt caaaaagctg gtgccaatga tcatcaggtt gggcagaaag acaaacaaca
      961 caagccaacc gacaatagtg acaatcacta cattctggaa cttacttgtg ttcttcatca
     1021 gccagtacga cctcccagct ttctacccaa ttaatggcca ttttttggtc gagagagtgg
     1081 tcaaagtcag gatcgtcttc attgaagaat tcgctgacca tcaccatctt gccattttcc
     1141 agttcaacaa ccgactccag cgtcatgcct ttgtagttac gctcgcgaac gtaaccaatc
     1201 agcccttcag cgtggttgtc gtcgttaatc tcttcaacac gtaagtcttc cgggcgcagc
     1261 agaacatgca gtttttgccc cggttcaacg gcgaagttaa cgtagatatt acattcgcgg
     1321 ccttcaacgt tggcgcgtac gcgctgctcg tcgagacgtt cgatgacagt ggcgttaaac
     1381 atattgattt cgccaatgaa cccggcaaca aacaggtttt tcggctcttc gtagatttca
     1441 cgcggcgtgc cgtcttgctc aatgcgacca tcgcgcatca ccacaatcct gtccgacatg
     1501 gtgagtgctt cttcctggtc gtgagtcacg aagacgaatg taatgccaag cttacgctgt
     1561 aacgctttca gctcgttctg catttgcttg cgcagtttgt aatccagcgc tgagagcgac
     1621 tcatccagca acaacagacg aggcttgtta accaccgcgc gagcaatggc gacgcgttgc
     1681 tgttgaccac cagagagctg atgcggttta cgttgagcga aggtttccag ctgcaccatc
     1741 cgcagggctt ccatcacgcg gggcgtaatt tcagcagcgg gggttttttg catgcgcaac
     1801 ccaaaggcca cattttcgaa cacggtcatg tgggggaaaa gtgcgtagct ttggaaaaca
     1861 gtgttcacat agcggttttc cgccggaacg tgggtgatgt cctcgttatc caacatgatg
     1921 cgtccggaat caacagtttc cagacctgca atcaggcgaa gaacggttgt tttaccgcag
     1981 ccagaagggc caagcagcgt gaggaactcg ccattgttga tagtcagatc cagctgggga
     2041 atgacctctt taccatcaaa gcatttgcga attcccgcca attgcaccag cggtgaaagc
     2101 gaactcggtt gtttattcaa ttttttactc tgtcccatgt aaacgcaacg gatggcttac
     2161 cgatgcgggg tttgtggtta accaccttgg tgactcttaa tgagggcggt aattctacgg
     2221 caaaccgctt gaatcgccaa tctttgttgt gaattactgg cttagcttta tattcattaa
     2281 ggtaatgctg ataaatattc ccgcttgcag gggtaaaagt gacctgacgc aatatttgtc
     2341 ttttcttgct tcttaataat gttgtcacaa aaagtgaggg tgactacatg gataaactac
     2401 ttgagcgatt tttgaactac gtgtctctgg atacccaatc aaaagcaggg gtgagacagg
     2461 ttcccagcac ggaaggccaa tggaagttat tgcatctgct gaaagagcag ctcgaagaga
     2521 tggggcttat caatgtgacc ttaagtgaga agggcacttt gatggcgacg ttaccggcta
     2581 acgtccctgg cgatatcccg gcgattggct ttatttctca tgtggatacc tcaccggatt
     2641 gcagcggcaa aaatgtgaat ccgcaaattg ttgaaaacta tcgcggtggc gatattgcgc
     2701 tgggtatcgg cgatgaagtt ttatcaccgg ttatgttccc ggtgctgcat cagctactgg
     2761 gtcagacgct gattaccacc gatggtaaaa ccttgttagg tgccgatgac aaagcaggta
     2821 ttgcagaaat catgaccgcg ctggcggtat tgcaacagaa aaacattccg catggtgata
     2881 ttcgcgtcgc ctttaccccg gatgaagaag tgggcaaagg ggcgaaacat tttgatgttg
     2941 atgccttcga tgcccgctgg gcttacactg ttgatggtgg tggcgtaggc gaactggagt
     3001 ttgaaaactt caacgccgca tcggtcaata tcaaaattgt cggtaacaat gttcatccgg
     3061 gcacggcgaa aggagtgatg gtaaatgcgc tgtcgctggc ggcacgtatt catgcggaag
     3121 ttccggcgga tgaaagcccg gaaatgacag aaggctatga aggtttctat cacctggcga
     3181 gcatgaaagg caccgttgaa cgggccgata tgcactacat catccgtgat ttcgaccgta
     3241 aacagtttga agcgcgtaaa cgtaaaatga tggagatcgc caaaaaagtg ggcaaagggt
     3301 tacatcctga ttgctacatt gaattggtga ttgaagacag ttactacaat atgcgcgaga
     3361 aagtggttga gcatccgcat attctcgata tcgcccagca ggcgatgcgt gactgcgata
     3421 ttgaaccgga actgaaaccg atccgcggtg gtaccgacgg cgcgcagttg tcgtttatgg
     3481 gattaccgtg cccgaacctg ttcactggcg gttacaacta tcatggtaag catgagtttg
     3541 tgactctgga aggtatggaa aaagcggtgc aggtgatcgt ccgtattgcc gagttaacgg
     3601 cgcaacggaa gtaagcgaaa agggatgcgg catatgatgc cgcatccggc ttaaatccaa
     3661 acttaccctt cgaagaacca atacccgcta ttgaccagcg ccgcgagcat cgcgaggaat
     3721 gacggatctt ccagcgcatc gccaaaattc tccgcggtca gcgcaatgtt gctggcgagt
     3781 gcatccagtg ccggacggtg cggggaatcg atcttctcac cattggcata cacgtcgtcg
     3841 ccaatgcgca atacgcgcag accacccagg cgcaccagca catcaccttg tttcagcgca
     3901 tcgtagattt catctggctg ataaggcggc tctggcggcg cgatatccag ttcatgacgt
     3961 gactgggata taaactcccc aaaccattgc ttaaagtgtt ccggctggtt gatcaattcg
     4021 agcatcatct cacgcagttt atccatctct tgcggcagaa catccgcagg atgagcgcga
     4081 ggtggaacat caggatcgct gtagtagttg ccgcccagtt cacgttgcag cacataatcg
     4141 gcaaatccac taatcagttc ccgcgtattt ggcgcgcgaa aacccacgga atagttcatc
     4201 gcattttcca gcgcgtagcc ttcatgcggg aatcctggcg gaatataaag aatatcacca
     4261 ggctccagct cttcatcgat gatggcttcg aacggatcga cctgtaacag atccggatgt
     4321 gggcagtgct gtttcatttg cagcttttcg cccactcgcc agcgacgacg accggtaccc
     4381 tgaatgataa acacgtcgta ctgatcgaga tgcgggccga cgccgccgcc cggtacagaa
     4441 aaagagatca tcagatcatc aatacgccag tccggtagtt cacggaacgg tcgcatcagc
     4501 gcggcggtcg gctcatgcca gtggttcact gcctgtacca gtaatgacca gttggtttca
     4561 ccgagatgat cgtagctttc gaacggaccg tggctgacct gccattttcc atcctggtga
     4621 ctgaccagtc gactgtcgac ttcgctttcc atcgccagac ccgccaactc gtctggagag
     4681 atcgggtcaa taaaattatt aaagccgcgt tttaacacca ccgggcgttt ctgccagtga
     4741 cgttcaagaa aatcgggcca gttaagagtg agttggtatt ccatgttaag catccacagg
     4801 ctggtatctg caaccgatta taacggatgc ttaacgtaat gcgtgaagta tggacatatt
     4861 ttatcaatta gtgttttttg ccgtttcata atgtttcaga ttgttcatta tgtttttgat
     4921 tataaacaga tttgtgattt tccttgttcc agcttgtttc acgttgtagc cctttgtgca
     4981 acgatcggtg tagttaatgg tgtagttaat tgtgatgacc ggaatcaaaa ttatgagcag
     5041 agcacttaac aaactgagcg atacacagct gaggaaaatc aacggcacac ccgcccaaaa
     5101 aacagccttt cttaatgacg gtggaaacct gagcgtcagg cattcaacca gtggcctttt
     5161 aacctggtat ttcacttaca gggccggaac gggaaggggg gcaccaccgg aacgcattaa
     5221 gctgggaaat tatcctgatc tgagcctgaa atcagccagg gaaaaagccg cccagtgtcg
     5281 cgcatggctg gcagagggga aaaatccacg tcatgagctt aattacaccg tacaggaagc
     5341 gttaaagccg gtaacggttg gcgatgcgct cacctactgg cttgagtcgt acgcaaagga
     5401 aaaccgcgtg gattatgccg ccctgaaaaa gcgccttaat aatcacgtaa tacagcacat
     5461 tggtgctatg ccgctggata aatgcgagct acggcactgg ctggcctgtt ttgaccaggt
     5521 ggcaaagcga acgcctgtta ctgccggatt cttgctacag acgtgcaaac aggcgcttaa
     5581 gttctgccgg aggcggcgct atgcaatcag caacgttctt gatgatatga gtgtggcgga
     5641 cgttgggaaa aaaccggata taagcgagcg tgtcttaagc accaaagaac tgggcgaatt
     5701 attgcaggca ctggacaaaa aaatattctc cccctactac atcgcgttaa tccgcctcct
     5761 gattgtgttc ggatgccgga cggtagaact gaggttatcg gagatcagcg agtgggattt
     5821 taccgaaatg ctctggaccg ttccgaagga gcacagcaaa acgaaggtag ccatattccg
     5881 gcctataccg gaagcaatac tgccgttcat cacgcagctg gtggagcaga acaggcacac
     5941 gggcttattg ctgggggaag tgaaacagga ggccagcgta tcgcagtatg gaagattagc
     6001 gcacaggagg cttaatcacc ctcactggtc actgcatgac atccggcgca cctttacaac
     6061 catgctgaac gatttaggcg ttgatccgca tgtcgtggag cagcttacag gccaccagat
     6121 gccaggaatg cagcgagttt ataatcattc ccgttatctg gatgcgaaac gcaatgcgct
     6181 ggatatgtgg acggagcggt tagggatact ggcgggaaca catgaaaacg taaccacgct
     6241 accagtagcc agaagaaaat aatttttttc gtgttttttc agtatgcgca tactggatat
     6301 gcgaacagat acaacgtgca acaatgagca acaatgtgga acaactacga acaagaggcg
     6361 aaaaagcgta cggatttata agtagctgat ttttaatgtg ttactggttt tttatacact
     6421 agcgaatcac tctttaaaga gcgagaaaaa aggcatggaa caatatttta tgcctttgta
     6481 ttaacatgaa tttaaaagat tttattctta ctttttcatt ttctgtaaat catacatcct
     6541 cagtatttgc cattcatcac tctagcggga acaatacgac acaataagac acctgatgac
     6601 tctttaagaa acgaaagggg gcaatagtgt taagcactga tcggtttata cgtgaaaaag
     6661 aatgcgaaaa actaaccggc cttagccgta cgtgtcgcta ccgcctggaa aaggccggac
     6721 aattcccatc acgtcgtaaa cttggcggtc gttccgttgg ctggtcttta tccgaggttc
     6781 tggcctggaa ggatagctgc aaggcagttc attaatcacg ctggcggcac acagccgcca
     6841 cacatcaatc atctgaacac agagctataa ccatgaagat tgaatatacg ccagaacgtg
     6901 ggcggggatt cgttcgccct ggtgagactg gaaaaccaca aaattggggt ttttcaggca
     6961 taaaaaaagc ggccccgaaa tggagccgcc tttctgaaca gataacccgc tgcgccttat
     7021 gtgtatatga tcccaaacat aagcacgggg atgatagccg ctatcaggct ggtgggcaat
     7081 gctatcagtc tggttcagtt cgttgccata cctgcaatgg gcgcgtttcc ctgtactctt
     7141 taaggaattg ctcaagggca aaagcacatg gcgcgaatct ttctgattca tgctctatct
     7201 ttctgcgccg tcttttccgt gccggtgata atgttttggt caattcttta tcggtcattg
     7261 tgttgtcctg catagcaatg cgccgtaata cctcacacca cggcgctgat agtttttatc
     7321 ctttgggttc tatgccgcgt tgctgtaatt ctttgcggag aacgcgttta atccaagtgg
     7381 ctaaagatgt gtcgccatcg gccttagctg cttcttctag ctgcgctctg aagtcttccg
     7441 gtaatcgcat ttggtatgga ggcgaacgtt tttctaatgg tgttgacatg gtaattaccc
     7501 tttattacta tacctacatg gtaattacca ttgtacttgc cattacacaa aaatcaacgc
     7561 cccgcagtgc tgggaacaca tacggggcgt ctaaccaaca acgtaaacta ggagccgtta
     7621 tggttgccgt aaatcatata ccacaccttg tacacacaca aacggccttt gtgtggcgtt
     7681 ttctggcact gagtgccgga gaatctcaaa tcatccacgt aaccgcctgg acggaacgcg
     7741 aagcgcgtaa ccgttgcccg tccggttgtg ttgctgtatt tgccgcccgt attcgtcagg
     7801 aggtgcgcca tgattaatct gtcacttact gaccttaacc gcattcagtt tcgtgagaaa
     7861 ttcacagggc agctactggt caatgtggat aaaggtcgcg tggtgtgtaa ttaccactta
     7921 ccagatgaag caattgtcgc cacaagggaa tcattacagg aaatcacaaa acgtgccgga
     7981 atgattaaca cgaatattca ggggatgctt tatgcacaat aaaaccacac cggacgcagc
     8041 cgccgaagcc atcaaaacgc tgatgcacgc gctgattgat atttctgtta tcgcggacag
     8101 ggcacataag cacgccacca gcgaaacaga atatgccggt gctttcgttc ctcactcact
     8161 ggctgtcatg caatttagtg ctgatacggc gctgaatgag gccaaagcta tcctgattgc
     8221 tgattgtgaa aatggggggg ttatgcgtga tgatcgtttt aattccctga aacaggaatt
     8281 ttccggcgtt cctgatgatg cggctgatgc gctttcgtca atgccagaac ttattagagc
     8341 ggcttttttc ttactttcca cgagagaata taaatcaacg gggcttgatg tactgaatat
     8401 cgccgccgat tatgcggaat atgtggcaga ggcgcgttat agaagaaaat ttcctgagga
     8461 tgtaagccat gcgtgatatt taccaccaaa caatagaccg cgcatttctt gcactttctc
     8521 acagtgaaaa catgatggaa atattgcgca tatggcttga aacacttggc gacaatgaac
     8581 gcgacaaaca aaaatcaaga attgccacgg cattaataac gcttcttgag cctgtaataa
     8641 tggaactgca agaaatagat ctattgcacg acagatataa agaacagcac accggagaat
     8701 aaaaataatg aaacttaaat attctggctt aactgccagt ggcaacactc accctaaatt
     8761 tacgcgcggt gatatttacc gcgaccagta cggcggcacg gtaatgatta agggcgtgga
     8821 agaacggcgc gtaacctacc gccgtgaagg ttacgaatat gattgcgtga tgcctgttta
     8881 tcagttccgg cgtgattttt ctctggtaca gaccgcgccg cataacgtgc ccaccagcaa
     8941 cgccagggca cgggcaaaca tccagaagct gaaaaccatg gttaacggat tcaggggcaa
     9001 gaaatgaaaa gcgcaccgaa cttaaaaaaa cagccttacg acaagatgac cgaagtcatt
     9061 atttttgcgg gtagtgatgc ctgggcacat gcgaaacagt ggcaggaaca ggacgggcga
     9121 ctggctggcg ataatgtgcc tcccgttgtg ctggctgatg atcaactgga tgaactggca
     9181 gacctgagaa tcatcgacga ggggcgctat tgtgtccggc tgtacaaggc aggccacatc
     9241 aggccatcaa atattaatgc cattgcgcac aagctggcgg cggcgggtgt aactgatgcg
     9301 aattattacc ccgaagggat gcacagccat atgcgggaga actggcgcga atacctggaa
     9361 cgggtgcgcg ggaaagagcc ggcggaagaa aaaaaccacc agcgaaaaac cacgctaccg
     9421 atgagcgttg gatctaccgg atacgacacg caactggatt acgtggttaa ggggattatt
     9481 ccggcggtat cgctatgcag catatacggg gctagcgggt cctataaatc attccttgcc
     9541 ggatcgtggg cgtgccatgt tgccactggt cgccagtggg gaggccgcag ggttgcacat
     9601 ggtgcggttc tctatgtggt tggtgaaggc ggtataggtg ttccgcgtcg tgtaaaagcc
     9661 tgggaggttg tgcacgatga gcaggtgaaa aatctgtatc tggtaaaccg ccccatcttt
     9721 ccggctgccc cgcttgatgt tgatgaaatg gttatcgctg cccgtcaggt ggagcgggaa
     9781 acgggtaaac ctgtacgcat gattattctg gatacgctgg cgcgttgctt tggtgggaat
     9841 gatgaaaatg attcccgtga tatgggggcg tttatccgtg gttgtgacga actgaaacga
     9901 cgcacagggg ccacggtgct ggtggttcac cattccggca aggatgagac gaaaggcgcg
     9961 cgcggttcca gtgcatttcg tgcttcgctg gatgctgaat accggatacg cagggaggac
    10021 gcaggaagcg aagcgctggt tatctcatgc accaaaatga aggacgcgga ggaactcaaa
    10081 gaagccgcat atgacttacg cgtggtggag ctttttaccg acgctgacgg tgaattaatc
    10141 acgtcgctgg tggtggtgga tgatccgcgc cctcctgttg aactggagcg catcgaggag
    10201 gcagggaaca agacggaaaa ccataccgcg ctatgggggt gcatccgttc acgcacacag
    10261 aacggcgaca agtgcacgat cccgctgtta cgtgatgaca tgaaaaagct ggggtatgaa
    10321 atgaaaaact tccggcgctg gctgtacaag ctggaaaaag atggggttat tcgtatcgat
    10381 ggggatgatg tagcgccgct ataaaagtga ggagcaaaag cgagggggat agaaagaggg
    10441 ccaaaattag cccgctctcc ctcacttttc gacctgtata catcctcaaa agtgaggggt
    10501 aaaaaaatac ttatgaaaca cacacataga aaaaccgaaa atcccaactg cgacgaagtg
    10561 agacgcttga aaaagtgagg cgaaaaagtg agaggttgcg agaaatgacc caaaaacgca
    10621 gagacagaac agagccaaaa tataaagcgt tagacatgac tgagcacacc ttaaaggtgg
    10681 caatcagaac gatagaccgc cacacgcggg aaggatacgc gaaggaacat cccgacctga
    10741 taagcgcatt catgaccacg gcggcggcaa actttgccac gctgacagaa cgggagattg
    10801 ccgaagcgga acaggtaaca accatcaacg ttaaaaccgg agaggtggaa ttatgacggc
    10861 acagatagcc gcttacgggc ggctggtgga cgacccgcag gtaaaacaga ccagcaaggg
    10921 cacaccgatg acgctggcgc gtatggcggt atctttgcca tgcagccagg cacaggacgg
    10981 acaggcgacg ttatggttat cggtcatcgc atttggcaaa caggccgact tcctggctaa
    11041 acatcaaaaa ggcgatgttg ccagcgtatc cggcacgatg caggtaagcc agtggaccgg
    11101 acagaacgga gaaacgcggc agggctggca ggttatcgca gacagcgtaa tcagtgcccg
    11161 cgcggcacgt ccaggcggga acagacgcaa aaccacaggc acacagggta atcagccacc
    11221 agcgggaggc gatgaccctt acggtgatga tatttcgttc tgagggggtg acgatggtac
    11281 atgaccgcat agcggaggaa ctggaggcga aaggctttta ccggagggcg gcggcgcgat
    11341 ggggtgaagt catgcagctg gtggagacag acaaggaacg gcatcacatc acgatgcgac
    11401 ggctggaatg ttcaaggaag gcacagaggg caccggagcc gccggataat ttcggagacc
    11461 tgaaaaaggc agtcgatcgc acttatgccg aaatgggtat agatggtgct ggtgatgaaa
    11521 tatggcgcaa ttaccaggac agctaatcaa acagccggag aaatccggct ttttttgcac
    11581 cagttgaaac ggtatggcgc attaccgggt tttcgtcacg gtcaggcata gttactatct
    11641 gaaacaaaca gacacaacag aggaaaaaaa caatgccgat gaaatttgat gagatattaa
    11701 aacagcgtga taaataccat gctgacaaca tggagacgat gagcatcaat gattaccgcg
    11761 cattcctgga gacgggcgca ctgattgaaa aggatcagca tggttttgtg agatgtgctc
    11821 tatccggtga aatgctggcg gtaaatcctg aacagataga tgcattgata gaatttctga
    11881 aagagatcag agactgagcc agcacacagc acacatagcc ggagcaatcc ggcttttttg
    11941 cgcccaaaaa aagcccgata aggtcagagg gttcttatcg ggcttttgca tatgaggttt
    12001 ttggatgcac tggcgttcgt gatcgggata atcatttcat aatttgcaac ataactcaat
    12061 atcattgcat aaaatgcaat tctgattata atcaggactg gataaacatc cagttatgat
    12121 tttttaagtc gaagaggaat ttcttactat ggctgaagag aaaaaaggcg gtgtttcggt
    12181 gtacataagc cccgacatcg tgaaggcgct caaggaacgc caccagcaga acgtaaaagc
    12241 aggcattgcg gcaggacttg atccgctggc gatggttgag ccgtcaacag gctggcaggt
    12301 ccgcgcctat ttacgcgcgg cgctgggcat gaatcagacg catggaggtg aataatggca
    12361 ggcaaagcaa cggcacttaa cactaaccag cttttcgcgt acctgaatcg cggggatatt
    12421 gcagaattta aattcagtcc gctgtttacc acgctgtttt tcccgaacgt ggcgaccttc
    12481 aacacccaga acatcatgct ggacaccctg gacattgaag aggttacgat gtcagcgttc
    12541 tgttcgccta tggtgggtag tcaggtccag cgcgataaag ggtacgaaac cagcacgatt
    12601 aaaccaggct acatgaaacc caaacacgaa atcgatccaa caaaaaccat catgcgcatg
    12661 gctggagaag atccggcaca gcttaacgat cctacctatc gccgtatgcg cctgattact
    12721 ggcaacatgc gccgccagat aaacgccatt aaggcacgcg tggaatggct ggcggtgaat
    12781 gcggtaacga ccggaaaaaa cctcattgag ggcgaaggca tagaacgcta tgaaatcgac
    12841 tggaagatcc cggaaagcaa tatcatagaa caggctgacg gtaaaaaatg gtctgagctg
    12901 gataaagaaa cacacgatcc aatctatgac atcgagcttt atgctgatca gggcaattgc
    12961 cccgcaaacg tcatgattat gggcgctgaa gtatggcgca cgttacgcag ctttaaaaaa
    13021 ttccgcgaac tgtacgacct ttcccgtggt tcagaatccg ccgcagaact ggcctgtaaa
    13081 aacctgggtg aagtggtgag ctttaaaggc tatctgggcg atattgccct tgtcgtctat
    13141 tccggcaaat acactgacag cgacggcacc gaaaaatatt tccttgagcc tgatttgctg
    13201 gtcctgggta acaccaacaa taaagggctg gtggcctatg gtgcgattat ggatcaggaa
    13261 gcggtcagaa cgggcgcaac gcaaaacatg ttttacccga aaaactggat tgaggacggc
    13321 gatccggcga ttgagtacgt gcagacgcac agcgcaccgc agccggtacc ggcagatatt
    13381 cgcaaatttg ttaccgtcaa aattggttaa cggggggatt ctatggacac tccatacatt
    13441 gagttatttg caggtagtca gcaggtatcc acgacgctgg tacattttgc tgctgatgct
    13501 ggcgttattc aggaatttac cccgctgatg ctggcggaaa atggcgagtt taagccgtgg
    13561 gatggtcagg aatctggcaa ggctgtttat ctgacctcgt accccgtgga cacgtcgacg
    13621 cagaaatcag cacagtgcta caagacgggg atattcaata ttgccgccgt taactggcct
    13681 gagagcgccg acaccgacgc gaaaaaatgc gccgcctttg cgggttctgg cgtatccgtt
    13741 cagccgctgg cgcgataagc agggggaacg atggcaacga atgaaagtat catgacgcta
    13801 ccgctggcga gtaaatttaa agccgaagcg cgggcaatgg ctgacagagg tttatcaacc
    13861 tacgaggccg tatatcaact taacagcctg gaagagaagg acaagccgcg cgctgatgcg
    13921 attatggcgc ttcatgaatc tgacgactat cagccgctgt tacgtgcaat ggcaaacgtg
    13981 ccttgtattg atgttgatac ggctaaaaac atcctgaaca tgaccataga gcaggaacgc
    14041 ccgaaggttg caccagagct taccgcagcc tttgaaaact ttatggacat gcacagccca
    14101 caagccgtat cagctggcat ggcatacgat ggcagaaacc cgggcgatga cggcgacatc
    14161 gatcgcatac tgaaaaccat ctgagacaag gccggagaaa tccggctttt ttgcgggtcc
    14221 tttccggcat atggacctgt tacggggcgg cgacctcgcg gattttcgct atttatgagc
    14281 cttttcaggg tggtggtggt ggttttgttg tttgatctat ctttatgaat gaaaagggaa
    14341 agatgcaagc aatacaccaa cctgaagcag taattaagtt ggtgtattaa tgaaatctca
    14401 tctgatgaac aaaaaaaaca tggcgaaaag ctgccgtgta agtgcgacag cgttcgacaa
    14461 gtggggagta actcccgttg agcgtaaagg ccgtgaggcg ttttatgatg ttgccagcgt
    14521 gatagaaaat agggttaaca atgcaattag ccagcttata aacgacaaag gcgagattga
    14581 cgatgatgaa cttttacgag tcaggatcag attactgaca gcacaggcag aggcgcagga
    14641 acttaaaaac gatcgcgatc gcggtgacgt gattgatact gagttttgcc tgtacgcgct
    14701 ttcaaagctg gcgagtcaga tttcatcaat catggacagc ctcccgctta ctatgcaaag
    14761 gagcttccca cagattaccc ccgccatgct ggacaggctt aaaagggaag tggttaaagc
    14821 ctgtaatgcc agtgccagag ttgccgacaa cctcccacag atactggctg attacttgaa
    14881 agaaacaacc ggaaacgtac cggaaaagtt gctacagaag aaaggcgagt aacagacgcg
    14941 caattattga acaaaaatga gaaaggatct gataatggca ttttctggct aaaatgcctc
    15001 gtgtcagatc ctttcttttt tttaatattt tcattgttaa acagtctgtt acagagaaga
    15061 agatcggatc tgacttttgc cggaaaattc tcataaacag ataaaaaccg cgaggtcgcc
    15121 gccccgtaac gggccataat tccaggaagg acccgacgac accagactat cagagcgatg
    15181 ggggcacaat gacagaagcc gaactactgg gattaatccg ccgcgtcgcc ggaatcagcc
    15241 agcagcatga cgaacaggcc acacagccgg acagcgtgac cgctgaaaat tatgtgcgtg
    15301 ttgtagcgga agtgatgcgc cgtgatggta tagaacttaa tggcgttgat atgcgcaaca
    15361 tacggaaccg cgttcttgaa atgctggcct acaatcgccg cgttgaactg tatcgggaga
    15421 aagaaaaaat aacgtaccac tggaagaagc cggaacggct gcggcggtaa cttgctgata
    15481 ttcccgataa cgcaaaattg cgctggctac atccctcaca tctgaggact gttaatcaga
    15541 aggccggaat atattcacaa actacgaaat tttcgtagtt taattaatgg tcagattaat
    15601 cgctaacccg ctgatatttt cggaaacctc aatttgagga agtcggaagc gtcacccttc
    15661 atattctgaa tggtcataat gaccacgcag accatcaagg caaccacaac gaacatcgtt
    15721 acggttggag cttttacccc actgggggaa aggtattacg ataatcataa cacctaccga
    15781 acaggtaaaa cccaccagcc tgattaacag ttaaccggaa aaaaaatccg gtagccccgt
    15841 tatggggatc cccatatcga cattaacgcc cctcatgaat tggtgcgctt ccccctggaa
    15901 agattacccg cctgtatatt tcttgtgtct atttgttcca cgttgtttca tacagtgcac
    15961 cgaacggtgt agttaatggt gtagtcaatt tgctgttttt agcacttttt gcagtgtggg
    16021 ttgtttaatt aaatcaatgg gttgccttgc tgtggtgata aaaggacata tttattcatc
    16081 tttcggcgca gaatgctggc gaccaaaaat cacctccatc cgcgcaccgc ccagcatgct
    16141 ctctccggcg acgattttac cctcatattg ctcggtgatt tcgcgggcta ccgccagccc
    16201 cacaccttgc ccagggcgta aagtatcaac ccgttgacca cggtcgaaaa tgacctctcg
    16261 cttgcttaat ggaataccgg gtccatcatc ctcgaccaca atatagagat gctcgtcggt
    16321 ttgccttgca gaaatttcga caaactcgag gcaatattta caggcattat ccagcacatt
    16381 gcccatcacc tcgacaaaat cgttctgctc accgacaaag ctgatctctg gcgaaatatc
    16441 gagagagata ttgacccctt tgcgttgata cactttgttc agcgccgagg tgagattgtc
    16501 cagcagtggg gcgaccggat gcagctcgcg gctgagcaat gtcccgccgc gcatactggc
    16561 acgatgcagg tagtagccaa tttgctgtga aatgcggctg atttgctcca gcattaccgg
    16621 ttcagcatca ctgacgctca tcttttcact acgcagagaa cgcagcgtac tttgcagcac
    16681 cgccagtggc gttttcagac tatgggtcag gtcggtgagc gttgtacggt atttgtcgta
    16741 acgttcgcgt tcgcttttta acaatcggtt caggtttcgt accagactgg tcagttctcg
    16801 cgttgtggct ggattgagca attcgcggtt atgttcttcc agttcgcgga cttcttttgc
    16861 cagggcttcg atggggcgta aactccacca ggcggcgacc cacagcagcg ggatcactaa
    16921 cagcagattg gctgagagca catagataaa ccagctccag accatatagg aactttttag
    16981 ctccaccgga atggtatcca ccaccacaat ggttaacttt ggcatccgcg atgttgccgg
    17041 gtagacgttt accgccaccg agtgggtcat ctccgcgtcg tcatcatctt cccgcacttc
    17101 ctgcaactgt tgctgtatcg aatgatctcc actcagcaag aggctggtat cgttaacatc
    17161 cgcttcaatt tcatgaaaac cattcgattt cagccagtca ggctggatca tcttcatcag
    17221 ccagggcacg tcacgttgcg cccataaaag ctgcccgttc tcatcataaa ttagcgtcat
    17281 ggtggggctt tgcttgtcga tattttcggg taactcgaca tgcaacttat tgttttccca
    17341 cttcgcaagg gtatagaaca gattgctctc gccacgtaac agccgaaacg tagttttatc
    17401 gaaactgacg ctataaccga tcagcgcgac cattccgtag gcaagcgaaa gcaccagtac
    17461 taccgctgcc gttgccaaca gaaaacgtac ccgcagcgag agcgggaaaa aaagacgcag
    17521 taattttttc atcagcgcaa ttcgaacaga tagccctggc cgcgaacggt ggtaatcact
    17581 tcttggggat attgtgcctg aatttttttg cgcagacgtc ccatcagtac atcaatggta
    17641 tggctttccc gcagctccgc atccggatag agttggagca ttaacgaatc tttgctcacc
    17701 actttgccat tattgcgtat caacgtttcc ataatggtgt attcgaacgc ggtcagtttg
    17761 atcacttcgt cattaataga taattcacgg cgagagagat caacctgaaa cggggggagc
    17821 gaaatgacct gtgaagccag gccgctatta cgccgcatta atgcctgcat tcgcgccatc
    17881 acctcttcaa tatgaaacgg tttagtcacg taatcatcag caccggcact taatacttcg
    17941 actttgtcct gccagctttc acgggcggtt aataccagaa tcggcagtga aacatcattg
    18001 ctacgccagc ggcgaatcag tgacagaccg tcctcgtctg gcaatccgag atcgacaatc
    18061 gcaatatccg gtaaatgttc attgagataa taatcggctt ctttggcatc ttctgcatca
    18121 tcgacctgat gaccagcatc ctgaatctga actttaaggt ggtgacgtaa caacgcattg
    18181 tcttcaacaa ccagtacgcg catttttatt tctccctgtc ttaattatta aaatagtgta
    18241 acgcgattat gtggttatgg gggtaaacat taaataaacc agcgggggag gggaggtaaa
    18301 gtgaaaaaat aaaaagcgga taatcttaat aagcaggccg gacagcatcg ccatccggca
    18361 ctgatacgag tttatttcag ctcatcaacc atggtgatgg cgcgaccaat gtagttagcc
    18421 ggcgtcatcg ctttcaggcg ggctttctct tcttctggca acgccagacc gtcgataaac
    18481 tgcttcatgc cttcggcgtc aacgcgctta ccacgagtca gctctttcaa tttctcgtac
    18541 ggtttttcga tgccatagcg acgcataact gtctggattg gttctgccag cacttcccag
    18601 ttgtgatcca gctcatccag cagatggtca cggttcactt ccagtttgct cacgcctttc
    18661 agggtggatt gatacgcaat cagcgcataa ccgataccca cgccgaggtt acgcagcacg
    18721 gtggagtcgg tcaggtcacg ctgccagcgg gaaaccggca gtttgcttgc cagatgctgt
    18781 aataccgcgt tggaaaggcc cagattccct tcggagttct cgaagtcgat cgggttaact
    18841 ttatgcggca tggtggaaga accaatctca ccggcgatgg ttttctgttt gaagtggtta
    18901 agagcgatat aaccccagac gtcacggtca aagtcgatca gaatggtgtt gaagcgcgca
    18961 acgcaatcaa acagttcggc aatgtagtcg tgcggttcaa tctgggtagt gtacgggttc
    19021 cactgaatac ccagcgaggt gacgaactct tcgctgaact gatgccagtc aacttccggg
    19081 taagcggcga tgtgggcgtt atagttaccg accgcgccgt tgattttgcc gaggatctcc
    19141 acctggttaa gctggcggta ctggcgctcc atacggtagg cgacgtttgc catctcttta
    19201 ccgatggttg acggcgtggc tggctgaccg tgggtacgag acagcagcgg gatatcgcgg
    19261 tactgaacgg cgagatcttt aatgccatca attagttgac gccagtacgg caggatcact
    19321 tcatcacgcg cggttttcag cattaatgcg tgggagaggt tattgatatc ttccgaagta
    19381 caggcaaagt ggatgaattc agaaaccgca tgcagttccg ggatctccgc cactttttct
    19441 ttcaggaaat actcaaccgc tttaacgtcg tggttagtag tacgctcgat ggttttgatg
    19501 cgtgcggcat cttcttcgct gaaactggcg acaattgcat caaggtaacc gattgcgtcg
    19561 gcagcaaaag caggaacttc cttgatcgct gcgtgcgcgg ccagtttttg cagccaacgt
    19621 acttcaactt gtacacggaa tttcagcaaa ccatattcgc tgaaaatccc gcgcagcgcg
    19681 ctgactttat cgccgtagcg tccatcgaca ggggaaacgg cggtcagtga ggataattcc
    19741 atagatcaca actccggagt taaatgagca agaatttgtt ttgcctgagt ggtcaggcga
    19801 ttacgagaaa acatcagttg cagacgtccg ccgccgacct ggtgccagag cacggcggcg
    19861 cgaatgcctg ccagcagggt tgcgcgaact ttcgcctgca cttgtgggct ttgcagtact
    19921 gcaggggaac cggtgacctg aatacgcggg ccaagcgggc tgatcacatc aacatagata
    19981 gcagccatcg cgctcatcag cgtttcggac tgtaaatcga agtgttcgag ctggcgttgc
    20041 aggccgttga tccggttgcc cagagtgtcg agcgcacctt tcgctgagga gagtttgcgc
    20101 tcaagcacca tcaggctgag tgtgtagcgg gttaattcgg cgtttaagcc ctggcgactg
    20161 ctggcattga gcacgccgag caaggtttcc agcccgacgc gcaggttggc ttcgctaccg
    20221 ccaaaaaccg ccagcgtcga gctggggttc atatcaataa tactgttgag tgagacgtgt
    20281 agcgcatcgg catcacaatg cccctggtga gcgagttgtt gcaccaggcg tgccgactga
    20341 caaataccgg ccagggcgag ggtgatgtca tagtaattct ttgccacgtt cactgcttcc
    20401 ttgttttaag taaagataat aatcagaccg gcagcggcag acgctgctca ataataccgc
    20461 caccgaggca cacttcaccg ttatagaaga cggcagactg gcccggcgtc acggcggcaa
    20521 ccggctcatc gaaaatcact tcaatgcgat catcgtccag cgccttgacg gtgcaaggga
    20581 tatcggtctg gcgatagcgg gtttttaccg tgcaacgcat agtgccggta aatggttcgc
    20641 gatcgaccca gtgcagctgc tgggcaatca gaccgacaga catcagacgc gggtgttcat
    20701 ggccctgcgc aacaatcaga atgttgtttt cgacgtcttt atcgaccaca taccacggtt
    20761 cttcggtgcc ttctttggtg ccaccgatac ccaggccttt gcgctgaccg agggtgtgat
    20821 acatcagccc ctgatgctca ccaatttcat cgccatcgac ggtaatgatt ttgcccggtt
    20881 gcgccgggag ataacggccc aggaactcgc ggaatttacg ttcgccaatg aagcaaatgc
    20941 cggttgagtc ttttttcttc gcggtgacca gaccaagatc ttcagcaatc ttacgcacct
    21001 gcggtttttc cagttcgccg accgggaaca ggctttgcgc aatctgctca tggctgagcg
    21061 tataaaggaa gtagctctgg tctttattgc tgtccagacc acgcagcagg cggctcttgc
    21121 catcgacatc ggcccgacga acgtaatgac cggtagcgat ataatcggca cctaaatctt
    21181 cggcggcaaa ttcgaggaag gctttaaatt tgatctcttt gttgcacaga atatccggat
    21241 tcggcgtgcg accggcttta tattcggcaa ggaacagttc gaagacgttg tcccagtact
    21301 cggcagcaaa gttaacggtg tgcagttcaa tgccgagctt gtcgcagaca gcctgggcat
    21361 cagccagatc cgctgccgcc gtgcaatatt cctcaccgtc gtcttcttcc cagttcttca
    21421 taaacaggcc ttcgacctga tatccctgtt gttgcaacag ccaggcagaa acggaggaat
    21481 cgacaccgcc ggacatgccg acgattactt tttttgcggt ttcagacatt ggatcactca
    21541 cgacattgaa cttcaaggcg gcgtattcta tcacgcagcc ttttagatga caccctttgt
    21601 aaaaggccag ttaaaatcgc caatcatctc cagcggatag cgttgcccgc tttgataaca
    21661 acgaatactt tccgccacca gtggcgaacg aagatttgac gcctgtaaaa tttcttcggc
    21721 gctaacccaa cggcagcagt cgatatcgct gtcatgaggc tgggtcgggc atatttgctc
    21781 aagctcaatg gcaaaaagga aacgcaaaaa cggcgtttta tctggcgcaa tccactgatg
    21841 catacgaata aagtgttgcg gctgcgcgct gatgccggtt tcttcccaca gctcacgggc
    21901 ggcggcttcc actaaggttt catccgcttc cagatgcccc gccggttggt tccataacgc
    21961 tttaccatta atcgtctctt caacgactaa aaatttgcct tctgcgtgca ccacgcaagc
    22021 aacggtaacg tgcggtttaa acatttttgc atccttaatc tgtcacttct cgccattcgc
    22081 cattagcaag attatccaaa gtgtaatcac ccatcgcata acgaatcagt cgcagcgtgg
    22141 ggaaaccaac atgggcggtc atgcggcgca cctggcgatt acgtccttca tataaggtga
    22201 tcttcagcca gctggtggga atacttttgc gttcacgaat tggtggattc cgcggccata
    22261 accacgcggg ttcgtcaacc agctccgcgc cggcgggcag ggtagggcca tcatttaagg
    22321 ttacgccatt gcgcaaggct tcaagtgcgt cttgtgtggg aatgccttcc acctgcacat
    22381 aatagatttt tccagtgcgt ttgcctggct gggttaaacg cgcctgtagt gcaccattat
    22441 tggtcagcac caataaccct tcgctatctc tgtcaagacg acctgctgca taaacgccct
    22501 gaaccgggat gaattctttt aatgttttgc gtccggcttc atcggtgaac tgcggaagaa
    22561 catcgtaggg tttattgaac aggatcacac gcgtgggctg gttttcaggt ttacgcctgg
    22621 tagaacgttg cgagctgaat cgcttaacct ggtgatttct aaaagaagtt ttttgcatgg
    22681 tattttcaga gattatgaat tgccgcatta tagcctaata acgcgcatct ttcatgacgg
    22741 caaacaatag ggtagtattg acaagccaat tacaaatcat taacaaaaaa ttgctctaaa
    22801 gcatccgtat cgcaggacgc aaacgcatat gcaacgtggt ggcagacgag caaaccagta
    22861 gcgctcgaag gagaggtgaa tggaaagtaa agtagttgtt ccggcacaag gcaagaagat
    22921 caccctgcaa aacggcaaac tcaacgttcc tgaaaatccg attatccctt acattgaagg
    22981 tgatggaatc ggtgtagatg taaccccagc catgctgaaa gtggtcgacg ctgcagtcga
    23041 gaaagcctat aaaggcgagc gtaaaatctc ctggatggaa atttacaccg gtgaaaaatc
    23101 cacacaggtt tatggtcagg atgtctggct gcctgctgaa acccttgatc tgattcgtga
    23161 atatcgcgtt gccattaaag gcccgctgac cactccggtt ggtggcggta ttcgctctct
    23221 gaacgttgcc ctgcgccagg aactggatct ctacatctgc ctgcgtccgg tacgttacta
    23281 tcagggcacc ccaagcccgg ttaaacaccc tgaactgacc gatatggtta tcttccgtga
    23341 aaactcggaa gacatttatg cgggtatcga atggaaagct gactctgccg acgccgagaa
    23401 agtgattaaa ttcctgcgtg aagagatggg cgtgaagaaa attcgcttcc cggaacattg
    23461 tggtatcggt attaagccgt gttcggaaga aggcaccaaa cgtctggttc gtgcagcgat
    23521 cgaatacgca attgctaacg atcgtgactc tgtgactctg gtgcacaaag gcaacatcat
    23581 gaagttcacc gaaggcgcgt ttaaagactg gggctaccag ctggcgcgtg aagagtttgg
    23641 cggtgaactg atcgacggcg gcccgtggct gaaagttaaa aacccgaata ccggcaaaga
    23701 gatcgtcatt aaagacgtga ttgctgatgc attcctgcaa cagatcctgc tgcgtccggc
    23761 tgaatatgat gttatcgcct gtatgaacct gaacggtgac tacatttctg acgccctggc
    23821 agcgcaggtt ggcggtatcg gtatcgcccc tggcgcaaac atcggtgacg aatgcgccct
    23881 gtttgaagct acccacggta ctgcgccgaa atatgccggt caggacaaag ttaacccagg
    23941 ctctattatt ctctctgctg agatgatgtt acgccatatg ggctggactg aagcggctga
    24001 cctgattgtt aaaggtatgg aaggcgcaat caacgcgaag accgtaacct atgacttcga
    24061 acgtctgatg gaaggcgcta aactgctgaa atgttcagag tttggtgatg cgatcatcaa
    24121 gaacatgtaa tctctacatg tgttaaatat tta
//
LOCUS       DAZUGK010000053        23984 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZUGK010000053 DAZUGK010000000
VERSION     DAZUGK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.053"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(220..1131)
                     /gene="gsiD"
                     /locus_tag="ACHY5W_001878"
     CDS             complement(220..1131)
                     /gene="gsiD"
                     /locus_tag="ACHY5W_001878"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFS4446784.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1134..2054)
                     /gene="gsiC"
                     /locus_tag="ACHY5W_001879"
     CDS             complement(1134..2054)
                     /gene="gsiC"
                     /locus_tag="ACHY5W_001879"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFS4446785.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2072..3610)
                     /gene="gsiB"
                     /locus_tag="ACHY5W_001880"
     CDS             complement(2072..3610)
                     /gene="gsiB"
                     /locus_tag="ACHY5W_001880"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFS4446786.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3630..5501)
                     /gene="gsiA"
                     /locus_tag="ACHY5W_001881"
     CDS             complement(3630..5501)
                     /gene="gsiA"
                     /locus_tag="ACHY5W_001881"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFS4446787.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5488..6453)
                     /gene="iaaA"
                     /locus_tag="ACHY5W_001882"
     CDS             complement(5488..6453)
                     /gene="iaaA"
                     /locus_tag="ACHY5W_001882"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4446788.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQEIRYIEALSAIVETGQQMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGISH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            6657..7892
                     /gene="moeA"
                     /locus_tag="ACHY5W_001883"
     CDS             6657..7892
                     /gene="moeA"
                     /locus_tag="ACHY5W_001883"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFS4446789.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7892..8641
                     /gene="moeB"
                     /locus_tag="ACHY5W_001884"
     CDS             7892..8641
                     /gene="moeB"
                     /locus_tag="ACHY5W_001884"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFS4446790.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8717..9379)
                     /gene="fsa"
                     /locus_tag="ACHY5W_001885"
     CDS             complement(8717..9379)
                     /gene="fsa"
                     /locus_tag="ACHY5W_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4446791.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GISTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9510..10409
                     /gene="ybiY"
                     /locus_tag="ACHY5W_001886"
     CDS             9510..10409
                     /gene="ybiY"
                     /locus_tag="ACHY5W_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFS4446792.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVESC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10415..12847
                     /gene="ybiW"
                     /locus_tag="ACHY5W_001887"
     CDS             10415..12847
                     /gene="ybiW"
                     /locus_tag="ACHY5W_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFS4446793.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12993..13808
                     /gene="ybiV"
                     /locus_tag="ACHY5W_001888"
     CDS             12993..13808
                     /gene="ybiV"
                     /locus_tag="ACHY5W_001888"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFS4446794.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13960..15225
                     /gene="ybiU"
                     /locus_tag="ACHY5W_001889"
     CDS             13960..15225
                     /gene="ybiU"
                     /locus_tag="ACHY5W_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFS4446795.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPQLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15466..17058)
                     /gene="ybiT"
                     /locus_tag="ACHY5W_001890"
     CDS             complement(15466..17058)
                     /gene="ybiT"
                     /locus_tag="ACHY5W_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFS4446796.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELLVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17277..18197
                     /gene="ldtB"
                     /locus_tag="ACHY5W_001891"
     CDS             17277..18197
                     /gene="ldtB"
                     /locus_tag="ACHY5W_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4446797.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18264..18362)
                     /locus_tag="ACHY5W_001892"
                     /pseudo
     CDS             complement(18264..18362)
                     /locus_tag="ACHY5W_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18431..19549)
                     /gene="ybiR"
                     /locus_tag="ACHY5W_001893"
     CDS             complement(18431..19549)
                     /gene="ybiR"
                     /locus_tag="ACHY5W_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFS4446798.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(19546..20013)
                     /gene="mntR"
                     /locus_tag="ACHY5W_001894"
     CDS             complement(19546..20013)
                     /gene="mntR"
                     /locus_tag="ACHY5W_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFS4446799.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20199..20327
                     /gene="mntS"
                     /locus_tag="ACHY5W_001895"
     CDS             20199..20327
                     /gene="mntS"
                     /locus_tag="ACHY5W_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFS4446800.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20599..22182
                     /gene="opgE"
                     /locus_tag="ACHY5W_001896"
     CDS             20599..22182
                     /gene="opgE"
                     /locus_tag="ACHY5W_001896"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS4446801.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     STKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22231..22746)
                     /gene="ompX"
                     /locus_tag="ACHY5W_001897"
     CDS             complement(22231..22746)
                     /gene="ompX"
                     /locus_tag="ACHY5W_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFS4446802.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22799..22864)
                     /locus_tag="ACHY5W_001898"
     CDS             complement(22799..22864)
                     /locus_tag="ACHY5W_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446803.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23099..>23984
                     /gene="rhtA"
                     /locus_tag="ACHY5W_001899"
     CDS             23099..>23984
                     /gene="rhtA"
                     /locus_tag="ACHY5W_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFS4446804.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5845 a         6550 c         5967 g         5622 t
ORIGIN      
        1 aattatcagc cttatcttta tcttttcgta taaactcagc atgttaatac atttaacaaa
       61 ataactgtct gaacgctgtg ccgctgcaaa aacagtccct tgctgcggct ttttaaccaa
      121 attacccgat tctacaaaat cagaagaaag ttacaacact tgcttagggc aataatttcc
      181 tgtgaggtga ttactctttc aagcaatatt caaacgtaac tatcctttaa ttttcggatc
      241 cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca gaaaaatagc
      301 cagggccgga aaaatagcga catgcggcgc gataaccata tccgctcgtg cctcattgag
      361 cattgctccc cactctggtg tcggcggttg cgcaccgagg ccgagaaatg acaggctggc
      421 ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag acgagacggt
      481 cccaggcaag atatgacgca ataaaatggt catatcgctg gcaccaatac tgcgcgctga
      541 ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccagtc gggcaaacgc
      601 ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc ccaacaccgc
      661 aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc aaatgcgcat
      721 gatcagccga tcccaccagc cttcataata tccagccaac aagcccagca acgtcccgat
      781 cgccgcaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg caccaaccag
      841 gacacggctg aaaatgtcac gccccagtga atcgacgcca aaccagtgct gcaaagaagg
      901 tccgttattc agattgtcat aatcaaaata attttcggcg tcatagggag cgatccagcg
      961 tgcaaaaata gccaccacaa tcaataaaat aacgaacaat gcggcggtca tcgccatatg
     1021 ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgtacctggt caggtttgac
     1081 cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca tccttacttg
     1141 tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt gataagaata
     1201 aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg catttctacg
     1261 gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac cacgatagaa
     1321 ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat catcgcgttg
     1381 cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct cgcggtacgc
     1441 atataatctt cgcttaaaac gtcgacaaat gacgcacggg taaagcgcgc catcacggcg
     1501 gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact gtctgctccc
     1561 acggtaggca gccagcccag ttcaacggag aatacctgaa ttaaaagcat ccccagagca
     1621 aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc cggccaacga
     1681 ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc ccagaccata
     1741 ctggttatgg tcagccacag cgttggcata aagcggctgg caatctcatc ggcaaccgga
     1801 cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat atagtgccag
     1861 aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc tataacctgc
     1921 gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat atgcacaaat
     1981 aaaaacacca gcaccgagac gataaacagc gttggaatca accccagtaa gcgtttgata
     2041 acgtaattaa gcattccact ccctgcgttg cttattgcaa atccgcgtct tcaaagctga
     2101 agccggtgtc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc gacaccagtt
     2161 tttctaccac cagcgggatc cacggcgact cttgccagat gatatcctgc gccgccttat
     2221 acaagcgggt cttttccgcc ggatcattag ttttcagtgc ctgagccaag aagtcatcca
     2281 cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg tggccagttt tgcgatgcaa
     2341 acaacggcga tagtgcccag tcagcttcgc cggttgaagc cgaccaacca gtgtagaaca
     2401 tccgcacgcc gctctctttt tgccctttac cttcaacttc tgccgcccgc tgtccggcat
     2461 ccatcgcagt cacctgggct ttaatcccga cctgcgctaa ctgctgctgg gtaaattgca
     2521 gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtattg aaaccgttgg
     2581 gatatcccgc ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc cacggtttat
     2641 aactttgcgc gtaggcgata ctcggcggta ccacaccagt agctggcgtt gcatagcccg
     2701 cgaaggccac tttcaccagc gccgggcggt taatggcgta attcagcgcc tcacggacct
     2761 tcgggttatc gaatggcttt tgcgtcacgt tcatactgat ataacgctgc ataattgacg
     2821 gactggccat caactcgata tttttgtttt tctccagcag tgcggcctgc tcgtaaggaa
     2881 tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg ttgttatccg
     2941 ccaccggacg ccatgttatg ctgtccagtt tgggcaatcc tggctgccag taacccgcga
     3001 attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac ggtccggttc
     3061 ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgccggt gaaatcatcg
     3121 cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg agggtaatct
     3181 ttaccgttgt cggatcgatc gcttccgttt tagcaatatt cttatacagg ttatagcgtt
     3241 taagatgatt cgccggatcg ctggcccggt ccagattcgc tttcaccgcc gcggcgttga
     3301 aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtaa gtaatgccgt
     3361 catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct ttatccagac
     3421 cgaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca ttcgcgtcat
     3481 acggatcgag cgtggtgaaa ttcgatccta ccgccaccac cacatctttg gcagcgaatg
     3541 cacaagatgc catcaacgct gtcgcaatgc ccagcgccac taacccacta cggtgtacag
     3601 ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta ttctgattgt
     3661 ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc cacctcttcg
     3721 ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg cggtcgttgt
     3781 cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg atgctgcgga
     3841 ttttcaaaaa ccgcgcgccg tgggccaatt tcaacaattt gcccgagata catcaccgcc
     3901 acacgatgac taatccgctc taccacggcc atatcgtggg agataaacag atacgcaatg
     3961 ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat agaaacatcc
     4021 agcgccgaaa cggcttcgtc ggcaatgatc acttttggat tcaatgccaa cgcgcgagca
     4081 atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca ggcatgttca
     4141 ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcgtc tttacctggc
     4201 aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg acgtgggtcc
     4261 agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgtaatgc ctgaagtttg
     4321 ccgggtgaca aggtatcgat tcgctgaccg ttaaagataa tttcgccgcc ctgcgattcg
     4381 accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc gcccaccagc
     4441 gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg cacttcccgc
     4501 gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgaccagatt acgcacccgt
     4561 aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggggcctg tttcgctgga
     4621 tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta accctttcat cgcaccaagt
     4681 tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg aaaaatctgt
     4741 tctacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg atcggcaatc
     4801 tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat ctctttttgc
     4861 aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc ggtggttggc
     4921 tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat cactcgctgg
     4981 cgcatcccgc cagagagttg atgcggataa cgtgaaagaa tggtttgtgc ctccggaatg
     5041 cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact ggcgttttga
     5101 tgcagacgaa ttgattcggc aatctgttca cccacagtaa atactgggtt cagcgatgtc
     5161 atcggctcct gaaatatcat cgccatatcc gcaccgcgca catgacgcat ttgtgcagcg
     5221 ctctgctcgc taagttcaat cacttcacga ctgcgccgcc gcaacagcat tttatcgcac
     5281 tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg ccagcgcagt cactgactta
     5341 ccggagccgg attcgccaac aattgccagc gtctcaccgc gttgcaggct aaaagagaga
     5401 ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattaag attttcaacc
     5461 gccagcacat taccggcatc aagttcatca ctgtgtggca acggcgtccc ctttttcacg
     5521 gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca ttccttcggt
     5581 gttaaacggt agcgcgacat tcccttcatg gtcgatagcg attaagccac cgctaccgcc
     5641 aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac ttaatccgcc
     5701 gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga cttcgcccgt
     5761 gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggccc ccactaaggg
     5821 actatcgcca actcgtccgg gtaatttatt ggtcattccg cccgtggacg tggctgccgc
     5881 cagattgccg tctaaatcca acgccacggc ccccacggtg cccattttct gtttttcatc
     5941 cagtggcgca ccgctatggt cgaggactgt tgccccttcc tcgcgcgctg ccagtagttg
     6001 ttcataacgc aaaggcgtgg agaaaatctc cggcgagacg ctctccatgc catgagcaaa
     6061 cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct ccatcaccag
     6121 ccgggcggca agaaccggat tacgcagatg gctaatgccc gccaccgccc ccgctttcag
     6181 tgtgttgcca tccatcacgc aggcgtccag ttcatgcgtt tcatcacgcg taaagacggc
     6241 gccaattccg gcgttaaaga gcggacactc ttccagcaag cgcaccgctt ctgtcaccac
     6301 atccagcgcg ctttcgcccg cttcgagcat ttgctgtccg gtttcaacaa tggcagacag
     6361 cgcctcaatg tagcgtattt cctgttgcag actcatctgc gcgcggctaa ttgcacctgc
     6421 gccaccatga attgcaatga ctgctttgcc catagtattc gtccattaag ggtggctcgc
     6481 cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt gaatatagaa
     6541 agatggatat ggcatgtaaa ggcaggaccc agcgctacgc ccgaggtggc atacagtctg
     6601 tggcgttttt ctgacataat aggcaaattc gattttgcct ccgcaggagt gttttcatgg
     6661 aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt tctcgcgtca
     6721 ccccactgac cgcccaggaa acgctgccac tggtacagtg ttttggtcgt attctggcga
     6781 gcgatgtcgt ttcgccgctt gatgttccgg ggtttgataa ctccgcaatg gacggctacg
     6841 cggtgcgttt agccgatatt gcctccaggc aaccgctgcc cgttgccggt aaatcctttg
     6901 ccggtcagcc gtaccatggt gaatggcctg cgggtacctg cattcgtatt atgaccggtg
     6961 cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa caaacggaca
     7021 atggcgtgcg ttttactgct gaagtccgta gcgggcaaaa tattcgccgt cgcggtgaag
     7081 atatctctgc aggtgcggtt gttttcccgg cggggactcg cctgactacc gcagagctgc
     7141 cagtaattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg cgtgtagcgc
     7201 ttttttctac cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac ggccaaatct
     7261 acgataccaa ccgtctcgcc gtacacctga tgttagaaca gttgggatgc gaggtaatca
     7321 acttagggat tatccgcgac gatccacatg ccctgcgcgc cgcatttatt gaagccgaca
     7381 gccaggcgga tgtggtgatc agttccggcg gtgtttcagt gggtgaagcg gattacacca
     7441 aaacgattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt aaaccaggta
     7501 aaccgttcgc gttcggtaaa ctcagcaata gctggttctg cggcctgccg ggcaacccag
     7561 tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaaa ctaagcggta
     7621 acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc ctgaaaaaaa
     7681 cgccaggtcg ccttgatttc cagcgcggcg tgctgcaacg caacgccgat ggcgaactgg
     7741 aagtgacgac cactggacat cagggttcac atatatttag ctcctttagc ctcggcaact
     7801 gctttatcgt actggaacgc gatcgcggca atgtggaagt gggcgaatgg gtggaagtag
     7861 aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc aggagatgct
     7921 gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg aggcgctaaa
     7981 agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt cgcagtatct
     8041 ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gactcggttt cgctctcgaa
     8101 tctgcaacgc cagacgctgc acagtgatgc tacggtcggg caaccgaagg tggaatccgc
     8161 ccgtgacgcc ctggcgcgga tcaacccaca tatcgcgatt acgccagtca atgcactgct
     8221 ggatgacgca gaacttgcag cattgattgc tgaacacgat ctggtgctcg actgtacgga
     8281 taacgttgcg gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg tgccgctggt
     8341 ttccggcgcg gcaattcgta tggaaggtca aatcacggtc ttcacttatc aggacggcga
     8401 accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcgttaacct gcgtagaagc
     8461 aggcgtgatg gcaccgttga tcggcgtaat tggttcgttg caggcaatgg aagcgatcaa
     8521 actgctggca ggttatggta aaccggccag cgggaaaatc gtcatgtacg atgcgatgac
     8581 ctgccagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt gcgggcagta
     8641 attgccacct ccaggcctga taagacgcgg cactccgcca cgtcatcagc aatttatgag
     8701 gatgcagaac gtccggttaa atcgacgttc tgccaaacgc tccctgccag tcctgctcaa
     8761 acttcgccac agcggcatca accgccggat agctaatcat ctgttgtgcc acatccagtg
     8821 gcagagtaat tgattcacat cctgccagta agcagtccag cgcctgacgc ggggttttga
     8881 aactcgcggc taacactttc gcacgcggcg catgcatttt caataactgg tgtaagtcgg
     8941 tcacagtctg aataccgcta ccgccctgag catcaatacg attaacgtaa ggcgcaacat
     9001 attccgcacc tgccagcgcc gacagcagcc cttgtgccgc gccatatacc gcggttccca
     9061 gcgtcgaaat cccttccgct tttaacatct taatcgctgc cagcccctcg gcggtcaccg
     9121 gaactttcac cacgatatcc gcaataatag aacgcagctt aagcgcgtca ttcaccatcc
     9181 cttcagcagt ggtagccatt acctgggcaa acagacgccc ctgaccgccc atcgcttcat
     9241 gaagttccgg aagcacaacc tccagcggtt ttttacccgc ggcgataatg cttgggttag
     9301 tagtcacacc tgccagcgga aaaatacgtg acagcgcctt caccgcaaca acgtctgaag
     9361 tatccagata cagttccata accatcctca aaatgtactt tttgtctgaa tgtagactat
     9421 cacgggaaaa ctctctgaac agttgacgca catcaatcta actttcattc gaaagtattt
     9481 taatgtttta acgaaacaga gaggcaacta tgattttcaa tattcagcgc tactcgaccc
     9541 atgatggccc cggtatccgc acggtcgtat ttcttaaagg ctgttcgctg ggctgccgct
     9601 ggtgtcagaa cccggaaagc cgcgcccgca cgcaggatct gctgtatgac gcacgactgt
     9661 gtctggaagg ctgcgagctg tgcgctaagg cagcgccgga agtgattgag cgcgcgctga
     9721 atggtttgct tattcatcgg gaaaagttaa ccccggagca tctgacggcg ttaaccgact
     9781 gctgcccgac acaggcatta accgtgtgtg gtgaagtgaa aagcgttgag gagatcatgg
     9841 cgaccgtttt gcgggataaa ccgttttacg atcgcagcgg cggaggctta acgctttcgg
     9901 gtggtgaacc ctttatgcag ccggaaatgg cgacggcgct gctgcaagcc agccacgagg
     9961 ccggcattca tactgcggta gaaagctgtc tgcatgtgcc gtggaaatat atcgcccctt
    10021 ctctgcccta tatcgatctg tttcttgccg atttaaaaca cgttgccgac gcgccgttta
    10081 aacagtggac cgacggtaac gccgcaagag tgctggataa cctgaaaaaa ctcgccgcag
    10141 cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg ctttaatgcc gacgaaacct
    10201 ctgtgaaagc cattaccgat tttgccgccg acgagctgca cgttggtgaa attcatttcc
    10261 tgccctacca cacgctgggc atcaacaaat atcacttact taatctgccc tatgacgccc
    10321 cggaaaaacc gcttgatgcg ccagaactgc tcgactttgc ccagcagtat gcctgccaga
    10381 aagggttaac cgcgacctta cgaggataac aaccatgacc acactgaaac tggacacgct
    10441 cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat attgtgaaac cgccggtttg
    10501 taccgagcgc gcgcagcact ataccgagat gtatcaacaa catctcgata agccgatccc
    10561 tgtacgtcgc gcgctggcgc tggcacatca cctggcgaat cgcactatct ggatcaagca
    10621 cgatgagttg atcatcggca accaggcaag cgaagttcgc gccgcgccga tcttccctga
    10681 atatactgtc tcgtggatcg aaaaagagat tgatgatctg gcagatcgtc ccggtgctgg
    10741 ctttgcggtg agcgaagaga acaaacgcgt tctgcatgaa gtgtgcccgt ggtggcgcgg
    10801 tcagaccgta caggatcgct gctacggcat gtttaccgat gagcaaaaag gtctgctggc
    10861 gaccggcatc attaaagcgg aaggcaatat gacctccggc gatgcgcacc tggcggtcaa
    10921 tttcccgctg ctgctggaaa aaggtcttga tggtctgcgc gagaaagtag cggaacgtcg
    10981 ctcgcgcatc aatctgacgg tgctggaaga tttacatggc gagcaattcc tgaaagcgat
    11041 tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt ttcgctgccc tggcgcgtga
    11101 aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa ctgctggcga tggcagaaaa
    11161 ctgcgatctt atcgcccacc aaccgccgca gactttctgg caggcgctgc aactgtgtta
    11221 cttcatccag ttgattttgc agatcgaatc taacggtcac tcagtatcgt ttggtcgtat
    11281 ggaccagtat ctctacccgt actatcgccg cgacgttgaa ctcaaccaga cactggatcg
    11341 cgaacacgcc atcgaattgc tgcatagctg ctggttgaaa ttgctggaag tgaacaaaat
    11401 ccgctccggc tcgcactcga aagcctctgc gggaagtcct ctgtatcaga acgtcaccat
    11461 tggcggacaa aatctggttg atggtcaacc aatggacgcg gtgaatcccc tctcttacgc
    11521 gatcctcgaa tcctgcggtc gcctgcgttc cactcagcct aacctcagcg tgcgttacca
    11581 cgcgggaatg agcaacgatt tcctcgacgc ctgcgtacag gtgatccgtt gcggcttcgg
    11641 gatgccggcg ttcaacaacg acgaaatcgt catcccggaa tttattaaac tcggtattga
    11701 accgcaggac gcttacgact atgcagcgat tggttgtatc gaaaccgccg tcggtggcaa
    11761 atggggctat cgctgtaccg gcatgagctt tatcaacttc gcccgcgtga tgctggcggc
    11821 gctggaaggc gggcgtgatg ccaccagcgg caaagtgttc ctgccacaag aaaaagcgtt
    11881 gtcggcaggt aacttcaaca acttcgatga agtgatggac gcgtgggata cgcaaatccg
    11941 ttactacacg cgtaaatcaa tcgaaatcga atatgtcgtc gacaccatgc tggaagagaa
    12001 cgtgcacgat attctctgct cggcgctggt ggatgactgt attgagcgag cgaaaagtat
    12061 caagcaaggc ggcgcgaaat atgactgggt ttctggcctg caggttggca ttgccaacct
    12121 cggcaacagc ctggcggcag tgaagaaatt ggtatttgaa cagggcgcga taggtcagca
    12181 acaactggct gccgcactgg cagatgactt cgacggcctg acgcacgagc aactgcgtca
    12241 gcgtctgatt aacggcgcac ctaagtacgg caacgacgat gataccgtcg atacgctgct
    12301 ggctcgcgct tatcagacct atatcgacga actgaaacag taccataacc cgcgctatgg
    12361 tcgtggtccg gttggcggca actattacgc gggtacgtcg tctatctccg ctaacgtacc
    12421 gtttggcgcg cagactatgg caacgccgga cggacgtaaa gcgcacaccc cgctggcaga
    12481 aggcgcaagc ccggcttccg gtactgatca tcttggccct actgcggtca ttggctcggt
    12541 gggtaaactg cctacggcag cgattctcgg cggggtgttg ctcaaccaga aactgaatcc
    12601 agcaacactg gagaacgaat ctgacaagca gaaactgatg atcctgctgc gcaccttctt
    12661 tgaagtgcat aaaggctggc atattcagta caacatcgtt tcccgcgaaa cgctgctgga
    12721 tgcgaaaaaa catcccgatc agtatcgcga tctggtagtg cgtgtcgcgg gttattccgc
    12781 cttcttcacc gcgctctctc cagacgctca ggacgatatc atcgcccgta ccgaacatat
    12841 gctgtaatcc cctcagcccg gcggagacgt cgccgggcta aatcattcac accatcaatt
    12901 tcactctcac tttcattcga aatataattt gtgctctgcg tcacattgtt attacactgt
    12961 tacggtttca ttttcgagcc aggagcacac ctatgagcgt aaaagttatc gtcacagaca
    13021 tggacggtac ttttcttaac gacgccaaaa cgtacaacca accacgtttt atggcgcaat
    13081 atcaggaact gaaaaagcgc ggcattaagt tcgttgtcgc cagcggtaat cagtattacc
    13141 agcttatttc gttctttcct gagctaaagg atgagatctc ttttgtcgcg gaaaacggcg
    13201 cactggttta cgaacatggc aagcaactgt tccacggcga actgacccga catgaatcgc
    13261 ggattgttat tggcgagttg ctaaaagata agcaactcaa ttttgtcgcc tgcggtctgc
    13321 aaagtgcata tgtcagcgaa aatgcccccg aagcattcgt cgcactgatg gcaaaacact
    13381 accatcgcct gaaacctgta aaagattatc aggaaattga cgacgtactg ttcaagtttt
    13441 cgctcaacct gccggatgaa caaatcccgt tagtgatcga caaactgcac atagcgctgg
    13501 atggcattat gaaacccgtc accagtggtt ttggctttat cgacctgatt attcccggtc
    13561 tacataaagc aaacggtatt tcgcggttac tgaaacgctg ggatctgtca ccgcaaaatg
    13621 tggtagcgat tggcgacagc ggtaacgatg cggagatgct gaaaatggcg cgttattcct
    13681 ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc ccgttacgct accgatgata
    13741 ataatcatga aggcgcgctg aatgtgattc aggctgtgct ggataacaca tcccctttta
    13801 acagctgacc tctctaccgg agccatgcgc tccggttttc ccttcttttt acatcctgta
    13861 catcacatca aatcattaaa cttgcattaa cttatttata acttatattg tcacatgcaa
    13921 atcatcatag tttcgaatga aagttaatgc gaggtggtta tggcttctac ttttaccagc
    13981 gacacattgc ctgccgatca caaagcagct atccgtcaga tgaagcacgc gctgcgggcg
    14041 cagcttggcg acgtccagca gatctttaat cagctaagcg atgacattgc cacgcgagtg
    14101 gctgaaatca acgcacttaa agcacagggc gatgccgtct ggccggtgct gtcttatgcc
    14161 gatatcaaag caggtcatgt tactgcagag cagcgcgaac agattaaacg tcgcggttgt
    14221 gcggtgataa aaggccattt cccccgcgaa caagcgctag gctgggatca gtcgatgctg
    14281 gactatctgg accgcaaccg ctttgacgag gtctacaaag gccccggcga taatttcttc
    14341 gggacgctca gcgcttcacg tcccgagatt taccccatct actggtcgca ggcgcaaatg
    14401 caggctcgcc agagtgaaga aatggcgaat gcgcagtcgt ttctcaatcg tctgtggaca
    14461 tttgaaagtg atggaaagca atggtttaac ccggatgtga gcgtcatcta ccctgaccgt
    14521 atccgccgcc gtccgcccgg aacgacctcc aaaggtcttg gagcgcatac cgactccggg
    14581 gcactggaac gctggctgct tccagcgtat cagcgcgttt tcgccaacgt ctttaatggc
    14641 aatctggcgc aatatgatcc ctggcatgcg gcacatcgta cggaagttga agagtacacg
    14701 gtggacaaca ccaccaaatg ttccgtgttt cggacattcc agggctggac agcgctctct
    14761 gatatgctgc ctggtcaggg gttgctgcac gtcgtgccca ttcctgaagc catggcgtac
    14821 gtactgttac gtccgctgct tgatgatgtg ccggaggatg aactgtgcgg cgtagcgccc
    14881 ggaagagtat tgccggtatc agagcaatgg catccactgt tgattgaggc gttaaccagc
    14941 attccacaac tcgaagccgg agactccgtc tggtggcact gcgacgtcat ccattccgtt
    15001 gcccccgttg aaaatcaaca gggctggggc aacgtgatgt acattcctgc ggcaccgatg
    15061 tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg cactggaaaa aggcgcatcg
    15121 ccgggcgact tcccgcgcga ggactatgaa acaaactggg aaggacgctt tacgcttgcc
    15181 gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg tttgagagtt aatggaagtg
    15241 aattgatgtg ggattaagaa aatcgattcg acgttactca gattgctgac aagtgcgctt
    15301 tcttaatgcc tgatgcggcg ttaacgcctt atctggccta caaagtcctt cagattcaat
    15361 atattacaga tactgcgtag gcctgataag cgtagcgcat caggctattt tgcatttgtc
    15421 atcagccgcc gtcaccccga cagtatcggg gtgacggata ataacttact cgatcccttt
    15481 actacgcagg taatcttcgt aattaccgct aaagtcgatc acgcgttccg gggtgatttc
    15541 cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg tgtgaaacaa agatcagcgt
    15601 gccctgatac agttccagcg ccatgttcag tgactcgata gattccatat ccaggtggtt
    15661 ggtcggttcg tccatgatca gaatgttcgg cttctgcatc attaacttac caaaaagcat
    15721 ccgccctttt tctccaccgg aaagcacttt agctggcttt ttgatgtcgt cctggctgaa
    15781 cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca tcgccttcct gcttccactg
    15841 gctcatccat tcgaacacgg tcagatcatt ttcaaactca tattcgtggt cctgagcata
    15901 gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg ctgtccggtt gcagatcgcc
    15961 caccagcgtt ttcagcagcg ttgatttacc gacgccgttg gtacccagta ccgccagttt
    16021 ttcaccgact tccagcagca ggttgagatt tttaaacagc ggaccgttat caaacccttt
    16081 ggtcagacct tccacttcca gcgcgttacg gaacagcttc ttatcctgtt caaaacggat
    16141 gaacgggttc tgacggctgg aggctttcac ctcttccagt ttaattttat caatctggcg
    16201 cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg ctaaagcggc taacgaaaga
    16261 ttgcagctca gcaatctgcg ctttcttctt ggcgttatca gccagcagac gttcacgcgc
    16321 ctgggtcgcc gccgtcatgt actcatcgta gttgcccgga taaacgagca gctcgccgta
    16381 atccagatcc gccatgtggg tacagaccat gttaaggaag tgacggtcgt gcgagatgat
    16441 gatcatggtg ctgtcacgct cgttcagcac ctgttccagc cagcgaatgg tgtcgatgtc
    16501 gaggttgttg gtcggttcgt cgagcaggag aatatccgga tcagcaaaca gcgcctgcgc
    16561 cagcagcaca cgcagcttcc agccaggagc aacttcactc atcgggccgt agtgctgttc
    16621 cactggaatt cccacgccaa gcaacaattc accggcgcga gcttccgcag agtaaccgtc
    16681 catttcgccg tatttaactt ccagatcggc cactttatag ccgtcttctt cactcatttc
    16741 cggcaaagca tagatacggt cgcgctcctg cttcacttcc cacaactctt tatgccccat
    16801 gatcaccgta tccagcacag tgaactcttc aaaggcaaac tgatcctgac gcagcttacc
    16861 aatgcgctcg ttgggatcga gggaaacgtt acccagcgtc ggctcaaggt cgccgccgag
    16921 gatcttcata aaggtggatt taccactacc gttcgcgcca atcaggccgt aacggttgcc
    16981 gccgccaaat ttgacggaaa tgttttcaaa caacggctta ctgccgaact gcatggtgac
    17041 gttactggaa actaacacag gcgtatcctg aaaagagata tgacaaaccg cgcattatgc
    17101 cataagtctg aaagaagatc ctgctcctcg cttaaggtct acactcaagc caaaggcaaa
    17161 aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg aatgaactta taatgcgctt
    17221 ccaatactct aatattctca acccaatggc ctgccaggca caaaatctcg cttaacatga
    17281 atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg
    17341 cctctgcggt aacttatcct ctgccaaccg acgggagtcg cctggttggt cagaatcagg
    17401 tgatcaccat tcctgaaggt aacactcagc cgctggagta ttttgccgcg gagtaccaga
    17461 tggggctttc caatatgatg gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg
    17521 gtactgtcct gaacattccg cagcagctga tcctgccgga taccgttcat gaaggcatcg
    17581 tcattaacag tgcagagatg cgtctgtatt actatccgaa agggaccaac accgttatcg
    17641 tgctgccgat cggcattggt cagttaggca aagatacgcc tatcaactgg accaccaaag
    17701 ttgagcgtaa gaaagcaggc ccgacctgga cgccgaccgc caaaatgcac gcagagtacc
    17761 gcgctgcggg cgaaccgctt ccggctgtcg ttccggcagg tccggataac ccgatggggc
    17821 tgtatgcact ctacatcggt cgcctgtatg ctatccatgg caccaacgcc aacttcggta
    17881 tcggcctgcg tgtaagtcat ggttgtgtgc gtctgcgtaa cgaagacatc aaattcctgt
    17941 tcgagaaagt accggtcggt acccgcgtac agtttattga tgagccggta aaagcgacca
    18001 ccgagccaga cggcagccgt tatattgaag tccataatcc gctgtctacc accgaagccc
    18061 agtttgaagg tcaggaaatt gtgccaatta ccctgacgaa gagcgtgcag acagtgaccg
    18121 gtcagccaga tgttgaccag gttgttcttg atgaagcgat taaaaaccgc tccgggatgc
    18181 cggttcgtct gaattaatct tcaaaactta aagcaaaagg cggagtgata atccgcctga
    18241 tgctgctgat ggcaatacat aattgcctga tgcgctacgc ttatcaggcc tacgcagaac
    18301 ctgcaatata ttgaatccgc aagtttttgt aggccggata aggcgttaac gccgcatccg
    18361 gcatgaacaa agcgcgtatt accaacaatc agaggcggac taataatccg cctttttaat
    18421 ttgccagacc ttatcccgcc gggatcataa ccaacaaaac atatcccacc agcgccgccc
    18481 acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca
    18541 tacgtagcgc aatcaaattt gccagcgatc cgggtaataa cccaaagcca cctacgttta
    18601 ccgcccatgc cagtaataaa gacggcggca catagttcag caacaatatg gtactcggca
    18661 cgttactgat cacctgcgat aaaccgattg ccgttaacca taatccgggt tcagataaat
    18721 gactcacgtt acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tgaacgtcga
    18781 taaacatcgc cataaacacc agcagcagcg tccagtccac actgagcacc acgcgacgtg
    18841 ccagcagcgc aaagcccacc gccacaatca ccagtcccca cagctcttgt tttaactcca
    18901 gcgccgtcag aaagacgata tacagtccca gacaactcca caccagccgc ggtttccact
    18961 ccggcgtttg cacccccgta tggtattgga gtgcctttcc agggaaacaa caccagcaca
    19021 acagcaggag cgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg
    19081 caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca
    19141 gtagcgaacc agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct
    19201 cacacagcct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg
    19261 tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg
    19321 tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac
    19381 ctttggtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag
    19441 gccaggattt cggtgcaaag ggaacgaaaa aacttaatcc gataccaaca agaattaata
    19501 actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    19561 tttctgggta aacaaacgaa aggcatccag cgtctcttca ctaacatggt gctccatgcc
    19621 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaataaga aattttcgac
    19681 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    19741 gcctcgccag gggatcattt caatcagccc catagttgcc agtcgcttaa gcattttagc
    19801 caccgtcggt tgcgaaactc ccaaacgagc agccatgtct acctggcgag cttcccccac
    19861 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    19921 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    19981 ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    20041 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    20101 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    20161 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    20221 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    20281 atatggtcaa caacaaaccg cagcaagata aaccttccga taaatagcgg cgtcgcggta
    20341 cgccgcttca ctcctgcttt catgcaggca taacgcgttt tggtctgaaa aaccccactt
    20401 tttgtcggat ttgcaatccc ctttgcaaaa gatttgttcg tcagtagttg acctgaacgg
    20461 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    20521 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    20581 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    20641 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    20701 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    20761 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    20821 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    20881 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    20941 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    21001 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt
    21061 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    21121 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    21181 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    21241 aagtatcaga cctatgtggt ggtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    21301 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    21361 gactacattg ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    21421 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    21481 cagacctggt ggttttccaa ccagggtcaa atcggcgaat acgatacagc catcgccagc
    21541 atcgccaaac gagcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    21601 agcaccaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    21661 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    21721 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacat cgtgctatct ctataccatg
    21781 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    21841 ttctcgctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    21901 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    21961 tccagcgacg ataaagcgca tcgtgtgatt aaagcccgcc gctcagccaa tgacttctta
    22021 ggctttttct cccagtggac gggaattaaa gcgaaggaaa ttaacatcaa atacccgttt
    22081 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    22141 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    22201 agaggcggat tttttatatc accaaagcga ttagaagcgg taaccaacac cggcaatcca
    22261 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    22321 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    22381 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    22441 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    22501 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    22561 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    22621 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    22681 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    22741 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    22801 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    22861 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    22921 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    22981 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    23041 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    23101 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    23161 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgggcgcacc
    23221 gggtgtcact gcgctgcgcc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    23281 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    23341 gggtgggatg aattatcttt tttatctctc tattcagacg gtaccgctgg gtattgcggt
    23401 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    23461 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctacctc tggggcaaga
    23521 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    23581 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtggcaat
    23641 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    23701 actctggcac tggtcggtta ttccattggg tctggcggtc gctattctct cgaccgctct
    23761 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    23821 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    23881 gacacccata cagctgctgg cgctcggcgc aattatcgcc gcttcaatgg gttctacgct
    23941 gacagtacgc aaagagagca aaataaaaga attagacatt aatt
//
LOCUS       DAZUGK010000054        23946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZUGK010000054 DAZUGK010000000
VERSION     DAZUGK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.054"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            225..560
                     /gene="yjdM"
                     /locus_tag="ACHY5W_001900"
     CDS             225..560
                     /gene="yjdM"
                     /locus_tag="ACHY5W_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4446805.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            718..1161
                     /gene="yjdN"
                     /locus_tag="ACHY5W_001901"
     CDS             718..1161
                     /gene="yjdN"
                     /locus_tag="ACHY5W_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFS4446806.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AEGCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1294..2082
                     /gene="phnC"
                     /locus_tag="ACHY5W_001902"
     CDS             1294..2082
                     /gene="phnC"
                     /locus_tag="ACHY5W_001902"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS4446807.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCLSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADDPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2107..3123
                     /gene="phnD"
                     /locus_tag="ACHY5W_001903"
     CDS             2107..3123
                     /gene="phnD"
                     /locus_tag="ACHY5W_001903"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4446808.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPDKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKLYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3229..4008
                     /gene="phnE"
                     /locus_tag="ACHY5W_001904"
     CDS             3229..4008
                     /gene="phnE"
                     /locus_tag="ACHY5W_001904"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4446809.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4029..4754
                     /gene="phnF"
                     /locus_tag="ACHY5W_001905"
     CDS             4029..4754
                     /gene="phnF"
                     /locus_tag="ACHY5W_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFS4446810.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4755..5207
                     /gene="phnG"
                     /locus_tag="ACHY5W_001906"
     CDS             4755..5207
                     /gene="phnG"
                     /locus_tag="ACHY5W_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFS4446811.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLADGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNTSRVDFFTMVRGDNA"
     gene            5204..5788
                     /gene="phnH"
                     /locus_tag="ACHY5W_001907"
     CDS             5204..5788
                     /gene="phnH"
                     /locus_tag="ACHY5W_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFS4446812.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQIASLSGGRMLRLTGAGIAEERMIAPQLPESILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5788..6852
                     /gene="phnI"
                     /locus_tag="ACHY5W_001908"
     CDS             5788..6852
                     /gene="phnI"
                     /locus_tag="ACHY5W_001908"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFS4446813.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6845..7690
                     /gene="phnJ"
                     /locus_tag="ACHY5W_001909"
     CDS             6845..7690
                     /gene="phnJ"
                     /locus_tag="ACHY5W_001909"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFS4446814.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7687..8445
                     /gene="phnK"
                     /locus_tag="ACHY5W_001910"
     CDS             7687..8445
                     /gene="phnK"
                     /locus_tag="ACHY5W_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFS4446815.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8556..9236
                     /gene="phnL"
                     /locus_tag="ACHY5W_001911"
     CDS             8556..9236
                     /gene="phnL"
                     /locus_tag="ACHY5W_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFS4446816.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9233..9955
                     /locus_tag="ACHY5W_001912"
     CDS             9233..9955
                     /locus_tag="ACHY5W_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4446817.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLAQLGLLDIL
                     SSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKNPAQALNLQDRGVIGEGNRADLV
                     LAHRKGNHIHIDHVWRQGKRVF"
     gene            9955..10512
                     /gene="phnN"
                     /locus_tag="ACHY5W_001913"
     CDS             9955..10512
                     /gene="phnN"
                     /locus_tag="ACHY5W_001913"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFS4446818.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQAQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10499..10933
                     /gene="phnO"
                     /locus_tag="ACHY5W_001914"
     CDS             10499..10933
                     /gene="phnO"
                     /locus_tag="ACHY5W_001914"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFS4446819.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            10935..11693
                     /gene="phnP"
                     /locus_tag="ACHY5W_001915"
     CDS             10935..11693
                     /gene="phnP"
                     /locus_tag="ACHY5W_001915"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFS4446820.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPCEDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENTLPSGFEAGFDGMEIGVA"
     gene            complement(11770..12099)
                     /gene="yjdP"
                     /locus_tag="ACHY5W_001916"
     CDS             complement(11770..12099)
                     /gene="yjdP"
                     /locus_tag="ACHY5W_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFS4446821.1"
                     /translation="MKPYSTLFLFSLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLDQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12168..12371)
                     /locus_tag="ACHY5W_001917"
                     /pseudo
     CDS             complement(12168..12371)
                     /locus_tag="ACHY5W_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(12512..14776)
                     /locus_tag="ACHY5W_001918"
     CDS             complement(12512..14776)
                     /locus_tag="ACHY5W_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4446822.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTESLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQIEG"
     gene            14890..16410
                     /locus_tag="ACHY5W_001919"
     CDS             14890..16410
                     /locus_tag="ACHY5W_001919"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS4446823.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMMLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16407..17381
                     /locus_tag="ACHY5W_001920"
     CDS             16407..17381
                     /locus_tag="ACHY5W_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFS4446824.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            17400..18341
                     /locus_tag="ACHY5W_001921"
     CDS             17400..18341
                     /locus_tag="ACHY5W_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4446825.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18396..19079
                     /locus_tag="ACHY5W_001922"
     CDS             18396..19079
                     /locus_tag="ACHY5W_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFS4446826.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19094..19954
                     /locus_tag="ACHY5W_001923"
     CDS             19094..19954
                     /locus_tag="ACHY5W_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFS4446827.1"
                     /translation="MPLICLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGSDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            19951..20952
                     /locus_tag="ACHY5W_001924"
     CDS             19951..20952
                     /locus_tag="ACHY5W_001924"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS4446828.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGSVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(20949..21656)
                     /locus_tag="ACHY5W_001925"
     CDS             complement(20949..21656)
                     /locus_tag="ACHY5W_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4446829.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            21947..23932
                     /gene="yjcS"
                     /locus_tag="ACHY5W_001926"
     CDS             21947..23932
                     /gene="yjcS"
                     /locus_tag="ACHY5W_001926"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFS4446830.1"
                     /translation="MNNSQLFRLSRIVIALTAASGMMVNTAYAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5360 a         6918 c         6659 g         5009 t
ORIGIN      
        1 tgtgaattaa aaaaagaaca agtagagcat taaccttatc ttaaataata aatggaggca
       61 aaaagattat tttctttttg cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag
      121 cagtaccccc gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc
      181 cgttttttgg cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact
      241 gcccaaaatg caactccgaa tacacctacg aagataacgg catgtacatc tgcccggaat
      301 gtgcctacga atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag
      361 atgctaacgg caatctgctg gctgacggcg acagcgttac catcattaaa gatctgaagg
      421 tgaaaggtag ctcttcgatg ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg
      481 aaggcgacca taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg
      541 agtttgtgaa aaagaactga ttgtattgtg atcggtaagc cggataaggc gtttacgccg
      601 catccggcaa cggtgccaga tgcctgatgc gacgctcgcg cgtcttatca ggccgcctct
      661 catctgtata aatttcgaac tacacttaac tggcttctct taactgaggt caccatcatg
      721 ccgttaagtc cctacctctc ttttgccggt aactgtgccg acgcaattgc ttattatcaa
      781 cgtacgttgg gcgcggaact gctctataaa atcagcttcg gcgaaatgcc aaaatcagcg
      841 caggacagcg ccgaaggctg cccttccgga atgcaatttc ccgatactgc catcgctcat
      901 gccaacgtgc gtattgccgg aagcgatatc atgatgagcg atgctatacc atcaggaaaa
      961 gccagctact ccggctttac gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc
     1021 tggtttgata accttgccgc taacggaaaa atcgaaatgg cctggcagga aactttctgg
     1081 gcgcatggct tcggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcgtc
     1141 aaacaacaac caacgcaata atccgccggg aggcccgccc tcccgcactg tcatcgaatt
     1201 cccgttaact cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc
     1261 acgatggagc cactttttta gggaggctgc atcatgcaaa cgattatccg cgtcgaacaa
     1321 ctgagcaaaa ccttcaatca gcatcaggcg ctgcacgcgg ttgatctgaa tatccatcat
     1381 ggtgaaatgg tggctctgct tgggccgtcc ggttccggca aatccaccct tttacgtcat
     1441 ttaagcggtt tgattaccgg cgataaatcc gccggtagcc atatcgagct actgggccgc
     1501 actgtccagc gcgaaggccg tctggcccgc gatatccgca aaagccgcgc ccataccggc
     1561 tacatcttcc aacaattcaa cctggtgaac cgcctgagcg tactggagaa cgtgctgatt
     1621 ggcgcgctcg gcagcacgcc gttctggcgc acctgtttaa gttggttcac cggcgagcag
     1681 aaacagcgcg cattacaggc gctaacccgc gttggcatgg tgcattttgc ccatcagcgc
     1741 gtttccacgc tctccggcgg ccagcagcag cgtgtggcga ttgcccgcgc gctgatgcag
     1801 caggcgaagg tgattctggc cgatgacccc atcgcctcgc tggacccgga atccgcccgc
     1861 atcgtgatgg acaccctgcg cgacatcaat cagaacgacg gcatcaccgt ggtcgtcacg
     1921 ctgcatcagg tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgtcaggga
     1981 cacgtcttct acgacggtag cagccaacag tttgataacg aacgttttga ccatctctac
     2041 cgcagtatta accgcgtcga agagaacgcg aaagctgcct gacatcccca tcattgagga
     2101 aaacgaatga acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc
     2161 accctgttaa gcccggcaca cgccgaagag caggaaaagg cgctgaattt cggcattatt
     2221 tcaacggaat cacagcaaaa cctgaaaccg caatggacgc cgtttttaca ggatatggag
     2281 aagaagctgg gcgtgaaggt caacgccttc tttgccccgg actacgcggg cattatccag
     2341 ggaatgcgtt tcaacaaagt ggatatcgcc tggtacggca acctgtcggc gatggaagca
     2401 gtggatcgcg ccaacggtca ggtcttcgct cagaccgtcg cggcggatgg atcgccgggt
     2461 tactggagcg tgttaatcgt caacaaagac agcccgatca acaacctgaa cgatctgctg
     2521 gcgaagcgga aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc
     2581 gtccccggtt actacgtctt cgctaaaaac aatatctccg ccagcgactt caagcgcacc
     2641 gtcaacgccg ggcatgaaac caacgcgctg gccgtcgcca acaagcaggt ggatgtggcg
     2701 accaacaaca ccgaaaacct cgacaagctg aaaacctccg caccggacaa gctgaaagaa
     2761 ctgaaagtga tctggaagtc gccgctgatc ccaggcgatc cgatcgtctg gcgcaaaaat
     2821 ctttccgaaa ccaccaaaga caagctctac gacttcttta tgaattacgg caagacgccg
     2881 gaagagaaag cggtgctgga acgcctgggc tgggccccgt tccgcgcctc cagcgacctg
     2941 caactggtgc cgattcgcca gctcgcactc tttaaagaga tgcagggcgt aaaaagcaat
     3001 aaaggactga atgagcagga caagctggca aaaaccaccg agattcaggc gcagctggat
     3061 gacctggacc gcctgaacaa cgcgctaagc gcgatgagtt cggtgagtaa agcggtgcag
     3121 taaatcgtag gtcggataag acgccccggc gtcgcatccg acaatgtgca ggcgttgatg
     3181 ccggatgcgg tgcaagcacc ttatccggcc tacagaccgg agccaaacat gcaaaccatc
     3241 accatcgccc cacccaagcg cagctggttc tcgcttctga gctgggccgt tgttctcgcc
     3301 gtgttggtcg tctcgtggca gggcgcggaa atggccccgc tcacgctgat taaagacggc
     3361 ggcaacatgg cgactttcgc cgccgacttc ttcccgcctg atttcagcca gtggcaggat
     3421 tacctcaccg aaatggccgt cacgctgcaa atcgccgtct ggggcaccgc gctggcggtg
     3481 gttctctcca tccccttcgg cctgatgagc gccgaaaacc tggtgccgtg gtgggtttac
     3541 cagcccgttc gccgcctgat ggacgcctgc cgcgccatta acgaaatggt cttcgccatg
     3601 ctgttcgtgg tcgccgtcgg cctcggcccg ttcgctggcg tgttggcgct gtttatccac
     3661 accaccggcg tgctctccaa actgctttcc gaagcggtgg aagcgattga acccggcccg
     3721 gtggaaggca ttcgcgccac cggcgctaac aagctcgaag agatcctcta cggcgtgctg
     3781 ccgcaggtga tgccgctgct gatctcctac tccctctatc gcttcgaatc caacgtccgc
     3841 tcggcgaccg tcgtcggcat ggtcggcgcg ggcgggatcg gcgtcacgct gtgggaagcg
     3901 attcgcggtt tccagttcca gcaaacctgc gccctgatgg tgctgattat cgtcacggtc
     3961 agtctgctgg atttcctctc tcaacggttg cgtaagcact ttatctgatt agcgaggcat
     4021 tgatagctat gcacttgtct acacatccga ccagctaccc aacacgctat caggagatag
     4081 ccgcaaaact tgagcaggag cttcgtcaac actaccgctg cggcgactat ctccccgccg
     4141 agcagcaact ggcagcgcgc tttgaggtaa atcgccacac cctgcgtcgc gccatcgacc
     4201 aactggtgga aaaaggctgg gtacagcgcc gtcagggcgt cggcgtgctg gtgctgatgc
     4261 gcccgttcga ttacccgctc aacgcccagg cgcgttttag ccagaacctg ctggaccaag
     4321 gcagccatcc caccagcgaa aaactgctgt cagtattgcg tccggcttcc ggtcacgtcg
     4381 cagacgcact gggtatcgcc gagggggaga acgtcatcca cctgcgcacc ctgcgccggg
     4441 tcaacggcgt cgcgctctgt ttaatcgacc actactttgc ggacctcacc ctctggccga
     4501 cgctgcaacg cttcgacagc ggttcgctgc acgattttct gcgcgagcaa acgggaattg
     4561 cgctgcgccg cagccagacg cggatcagcg cccgccgcgc ccaggccaaa gagtgtcagc
     4621 gtcttgaaat tccgaatatg tcgccgctgc tgtgcgtgcg cacccttaac caccgtgacg
     4681 gtgaaagcag cccggcggag tactccgtca gcctgacgcg cgccgacatg attgaattca
     4741 ctatggagca ctgaatgcac gcagataccg cgacccgcca gcactggatg tccgtactgg
     4801 cgcacagcca accggctgaa ctggcagccc gcctgaacgc gctgaacatc accgccgact
     4861 atgaggtgat ccgcgccgct gaaaccggcc tggtacagat tcaggcgcgg atgggcggca
     4921 ctggcgaacg cttttttgct ggcgacgcca cgctcacccg cgccgccgta cgcctggctg
     4981 acggcacgct cggctacagc tgggtgctgg ggcgcgacaa acagcacgcc gaacgctgcg
     5041 cgctgattga cgcgctgatg cagcaaaccc gccattttca aaacttatca gaaaccctta
     5101 ttgccccgct ggacgctgac cgtatggcac gcattgccgc acgccaggcc gaagtgaaca
     5161 ccagccgggt cgatttcttt acgatggttc gcggagataa cgcatgaccc tggaaaccgc
     5221 ttttatgctt cctgtgcagg atgcccagca cagttttcgt cgcctgttaa aggccatgag
     5281 cgagccgggc gtgattgtcg ccctgcatca gctcaaacgc ggctggcagc cgctgaatat
     5341 cgccaccacc agcgtgctgc tgacgctggc cgataacgac acgccggtgt ggctttccgc
     5401 gccattaagt aacgatatcg tcaaccagag cctgcgtttt cataccaacg cgccgctggt
     5461 cagccagccg gaacaggcga ccttcgcggt aacggatgag gcgatttcca gcgaacagct
     5521 taacgccctt tccaccggca ccgccgttgc gccggaagcg ggcgcaacgc tgattttaca
     5581 gattgccagc ctgagcggcg ggcgcatgct gcgcctcacc ggcgcgggta ttgccgaaga
     5641 acgaatgatc gctccgcagc tgccggagag cattctgcac gaactcaccg agcgcccgca
     5701 cccgttcccg ctcggcatcg acctgatcct gacctgcggc gaacgcctgc tggctattcc
     5761 gcgaaccact catgtggagg tgtgctgatg tacgttgccg taaaaggggg cgaaaaggcg
     5821 atcgacgccg cccacgccct gcaagagagc cgacgccggg gcgataccga tttgcccgaa
     5881 ctgagcgtcg cccagattga acagcagctt aacctcgcgg tagatcgcgt gatgaccgaa
     5941 ggcggcattg ccgaccgcga actggcggcg ctggcgctga aacaggccag cggcgataac
     6001 gtcgaagcga tttttctgct gcgcgcctac cgcaccacgc tggcgaagct ggcggtgagc
     6061 gaaccgctcg acaccaccgg gatgcgtctc gaacggcgta tctccgctgt ttataaagat
     6121 attcccggcg gccaactgct tggcccaacc tacgactaca cccatcgcct gctcgatttc
     6181 accctgctgg caaacggcga agcgccaacg ctgaccaccg ccgacagcga acaacagccg
     6241 tcgccgcacg ttttcagcct gcttgcgcgt caggggctgg cgaagtttga agaggatagc
     6301 ggcgcacagc cggatgacat cacccgcacg ccgccggttt acccctgctc acgttcttcc
     6361 cgtttgcagc agttgatgcg cggcgacgaa ggctatttgc tggcgctggc ctactccacc
     6421 caacgcggtt acgggcgcaa tcacccgttc gcgggtgaaa tccgcagtgg ttacatcgac
     6481 gtgtcgattg tgccggaaga gctgggattt gcggtaaacg tcggcgaact gctgatgacc
     6541 gagtgtgaaa tggtcaacgg ttttatcgat ccgccggatg aaccaccgca ttttacacgc
     6601 ggctacgggc tggtgttcgg catgagcgag cgcaaagcga tggcaatggc gctagtcgat
     6661 cgtgcgttgc aggccccgga gtacggcgag cacgcgacag gcccggcgca ggatgaagag
     6721 ttcgtgctgg cgcatgccga caacgtcgaa gccgcaggct ttgtctcgca cctcaaactc
     6781 ccgcactacg tcgatttcca ggccgaactg gagctactca aacgtctgca acaggagcag
     6841 aaccatggct aatctgagcg gctacaactt tgcctacctc gacgagcaga ccaaacgcat
     6901 gatccgccgc gccatcttaa aagcggtggc gatccccggt tatcaggtgc cgtttggcgg
     6961 gcgcgagatg ccgatgccat acggctgggg aaccggtggc atacaactca ccgccagcgt
     7021 gattggcgaa agcgacgtgt tgaaggtgat tgaccagggc gcggatgaca ccaccaacgc
     7081 cgtatcgatt cgcaacttct ttaagcgcgt gaccggggtg aacaccaccg agcgcacgga
     7141 cgatgcgacg cttatccaga cccgccaccg catccccgaa acgccgctga cggaagatca
     7201 gatcattatt ttccaggtgc cgatccccga accgctgcgc tttatcgagc cgcgcgaaac
     7261 ggaaacccgc accatgcacg cgctggagga gtacggcgtg atgcaggtga agctgtatga
     7321 agatatcgcc cgcttcggtc atatcgccac cacctacgcc tatccggtga aggtgaacgg
     7381 gcgctacgta atggacccgt cgccgatccc gaaattcgat aacccgaaaa tggacatgat
     7441 gcccgccctg caactgttcg gcgcggggcg cgagaagcgc atctatgcgg tgccgccgtt
     7501 tacccgcgtg gaaagcctcg atttcgacga tcacccgttc accgttcagc agtgggatga
     7561 accatgcgcc atctgcggat cgacccacag ctatcttgat gaagtggtgc tggatgacgc
     7621 cggaaaccgc atgtttgtct gctccgatac cgattattgc cgccaacaga gcgaggcaaa
     7681 aaaccaatga atcaaccgtt actttcggtc aataacctga cccaccttta cgcgccgggc
     7741 aaaggcttta gcgatgtctc ttttgattta tggccggggg aagtgctggg cattgtcggg
     7801 gagtccggct ccgggaagac cacgctgctg aaatcaattt ccgcgcgtct gacgccgcag
     7861 cagggggaga ttcactatga aaaccgttcc ctctatggca tgagcgaggc cgaccgccgt
     7921 cgcctgctgc gcaccgaatg gggcgtagtg catcagcatc cgctcgacgg cctgcgccgc
     7981 caggtctcgg cagggggcaa tatcggcgag cggctgatgg cgaccggggc gcgtcattac
     8041 ggcgatattc gcgccaccgc gcagaagtgg ctggaagagg tggaaattcc cgccaaccgt
     8101 atcgacgacc tgccgaccac cttttccggc ggcatgcagc aacgtttgca gattgcccgc
     8161 aacctggtga cgcatccgaa gctggtattt atggacgaac caaccggcgg gctggatgtg
     8221 tcggtgcagg cgcgcctgct cgacctgctg cgcggtctgg tggtggagct gaatctggcg
     8281 gtggtgattg tcacccatga tctgggcgtc gcccgcctgc tggcggaccg tttgctggtg
     8341 atgaagcagg ggaaggtggt ggagagtggg ttaaccgacc gcgtgctcga cgacccgcac
     8401 catccgtata cccaactgct ggtgtcatcg gttttgcaga attgagccgg tgccggatgc
     8461 ggcgtaaacg ccttatccgg cctacaaatg cgctccccgt aggtcggata agacgcgtca
     8521 gcgtcgcatc cgacacccga accacgaggc gaaaaatgat taacgtacaa aacgtcagta
     8581 aaaccttcat cctgcaccag caaaacggcg tgcgcctgcc cgtcctcaac cgcgcctcgc
     8641 tcaccgtcaa cgcgggcgaa tgcgtggtgc tccacggcca ttccggcagc ggaaaatcaa
     8701 ccctgctacg ctcgctgtac gccaactatc tgcccgacga agggcaaatc cagatcaaac
     8761 acggtgacga atgggtcgat ctggtcaccg cgccagcgcg caaagtggtg gaaatccgca
     8821 aaaccaccgt tggctgggtg agccagtttc tgcgcgtcat cccgcgtatt tcggcgctgg
     8881 aagtggtgat gcagccgctg ctcgataccg gcgttccgcg tgaagcctgc gccgctaaag
     8941 ccgcgcgtct gctcacccgc ctgaacgtgc cggaacgcct gtggcatctg gcgccatcga
     9001 ccttctccgg cggcgaacag cagcgcgtca acatcgcccg cggctttatc gtcgactacc
     9061 cgattctgct gctcgacgaa cctaccgcct cgctggacgc caaaaacagc gccgccgtag
     9121 tcgaactgat ccgcgaagcc aaagcccgcg gcgcggccat cgtcggcatc ttccatgacg
     9181 aagccgtacg caatgacgtc gccgaccgcc tgcacccaat gggagcctct gcatgattat
     9241 caataacgtc aaactggtgc tggaaaacga ggtggtacac ggttcgctgg aggtgcagga
     9301 cggcgaaatc cgcgcctttg ccgaaagcca gagccgcctg ccggaggcga tggacggcga
     9361 aggcggctgg ctgctgccgg ggctgattga gctgcatacc gataacctgg ataaattctt
     9421 caccccgcgc ccgaaagtgg actggcccgc ccactcggcg atgagcagcc acgacgcgct
     9481 gatggtggcg agcggcatca ccaccgtact ggatgccgta gcaattggcg acgtgcgcga
     9541 cggcggcgat cggctggaaa atctggagaa gatgatcaac gccatcgaag agacgcagaa
     9601 acgcggcgtc aaccgcgccg agcaccgcct gcatctgcgc tgcgaactgc cgcatcacac
     9661 tacgctgccg ctgtttgaaa aactggcgca gcttggcctg ctggatatcc tctcttccga
     9721 ctactacccc gccagcctgc tggatgcggc gttccgcgtg gccgatgacc agagcaaccg
     9781 ctttacgctg ccgcaggcgg tgaggctggt gactaaaaat ccggcgcaag cgcttaatct
     9841 ccaggatcgc ggggtgattg gcgagggcaa tcgcgcggac ctggtgctgg cgcatcgcaa
     9901 gggcaatcac attcatatcg accacgtctg gcgtcagggt aaaagggtgt tctgatgacg
     9961 ggaaaactga tttggttaat ggggccgtcc ggctccggga aagacagcct gctggcggaa
    10021 ctccgcctgc gggaacaggc gcagctactg gtggcgcatc gctacatcac gcgcgatgcc
    10081 agcgctggaa gcgaaaacca tatcgccctg agcgagcagg agttttttac ccgcgcgggg
    10141 caaaatctgc tggccttaag ctggcacgcc aacggtctgt attatggcgt cggcgtcgag
    10201 attgacctct ggctgcacgc cggattcgac gtgctggtca acggctcacg cgcccatctg
    10261 ccgcaggcgc gggcgcgcta tgcatcggcg ctgctgccca tctgcttaca ggtgtcgcca
    10321 gagatcctgc gccagcgcct ggaaaaccgt ggacgcgaaa acgccagtga aattaacgcc
    10381 cggctggcgc gcgctgcccg ctatactcca caggattgcc atacgctcaa taatgacggt
    10441 agcctgcgcc agtcggtcga cacgctgctg acgctgattc atcagaagga gaaacaccat
    10501 gcctgcttgt gagcttcgcc cggccacgca gtacgacacc gacgcggttt acgcgctgat
    10561 ttgtgagcta aaacaggcgg agtttgacca tcacgcgttt cgcgtgggct ttaacgctaa
    10621 cctacgcgac ccgaacatgc gctaccattt ggcgctgctg gatggcgaag ttgtcggcat
    10681 gatcggcctg catttgcagt ttcatctgca tcatgtcaac tggatcggcg aaattcagga
    10741 actggtggtg atgccgcagg cgcgcggtct gaacgtcggc agtaagttac tggcgtgggc
    10801 agaagaagaa gcccgccagg ccggggccga aatgaccgag ctttcgacca acgtgaagcg
    10861 ccacgacgca caccgtttct atctgcgcga aggctacgag cagagccact tccgcttcac
    10921 gaaggcgctg taacatgagc ctgaccctca cgctcaccgg caccggcggc gcacagggcg
    10981 ttccggcatg gggctgcgag tgtgcggcct gcgccagagc gcggcgctca ccgcactatc
    11041 gccgccaacc gtgcagcggc gtagtgaagt ttaacgaggc gatcaccctg atcgacgccg
    11101 ggctgcacga tctcaccgat cgctggtcgc caggatcgtt ccagcagttt ttgctgacgc
    11161 actaccatat ggatcacgtc caggggctgt ttccgctgcg ctggggcgtg ggcgatccga
    11221 tcccggtcta cggcccgccg gatgaacagg gctgcgacga tctgtttaaa catccgggcc
    11281 tgcttgattt cagccacacg gtggaaccgt ttgtggtgtt tgatttgcag gggttacagg
    11341 tcacgcccct gccgctcaac cactcaaaac tgaccttcgg ttatctgctg gaaacggcgc
    11401 acagccgggt ggcgtggctg tctgacaccg ctggcttgcc ggaaaaaacg ctgaaatttt
    11461 tacgcaataa tcagccgcag gtaatggtga ttgattgcag ccacccgccg tgcgaggatg
    11521 caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct gaatcaggtt atccgctcgc
    11581 cgcgcgtgat tctgacgcac atcagccatc agtttgatgc atggctgatg gaaaacacac
    11641 tgccgtcggg gtttgaggcg gggtttgatg ggatggagat tggggtggcg tgatgagagg
    11701 gaatgtgcgt gctggccccc tcaccctaac cctctcccca gaggggcgat gcctgctcca
    11761 tacccaacat cattgcccat actcatcttc cattctccgc tcttcatctt ctagctgccg
    11821 acgctcctga tcaagctggt gctggcgatc gtccagttgc ctgcgacgat cttcaaactg
    11881 gcgacggcgg tcgtcatatt gtctgcgccg atcgtcgctc acttcacgct gccagccgtc
    11941 gtcgcgcgaa tcttcataat ctcgcccacg gtcagggtta taagcatcgt taatcgcctg
    12001 ctgaatattg ccaatggtgt cgtcgataat atcggcctgg gccggtacgg tggtcagcgt
    12061 gagcaggctg aaaagaaaaa gcgtggagta aggtttcata agccagtctc tgcgatggac
    12121 tggcttaacg ttagttgagt gtgaatgacc ggagtagcgg agtaatctca aattcggtaa
    12181 ggcgcagact cttccattag ctggttagct cgctgtttat tttccgccac cagcacaatg
    12241 ccatgacatt tttgatccgg tgaaatactc cagatacgct cttccggaat gcaaccgcca
    12301 tcgtagatca ctttcgtgcc atcataaggc caaaatgccc cttgttcagt gtctaataca
    12361 aacactgaaa tcgctatagg tggttggcat cacgatcata tgtgaatcgc ctcttgtcag
    12421 tactggcata attcctttta tatcccattc agaacagaat tgctcacaat agcagcacat
    12481 ttgcttcagg cctcttgata aattctgaaa atcatccctc aatttgcccc agcgcctgcg
    12541 ccaactgcgc acgggtaaag ggtttgcgca acagcgccac gtctggcagc gcggggttat
    12601 ggctggggcg taaatcctga ccgctgatga gtagcagcgt caggtgcggg taaagtttgc
    12661 gggccgcatt gaccacttct gcgccgctca tgccgccggg gagcattaaa tcgctgataa
    12721 aaatatcgat ttctgctgat gccgccagca gatccagcgc ctgttcgccg ctgcttgctt
    12781 ccagcgtcaa atagcccagc aggtgcaact gttcgcagat ggtctggcgc accgcggctt
    12841 catcttccag cactaacacc agtttatcgc cgctgacaga agcctgttct actgccgctg
    12901 gcgataggtt tttcgccgcc gtcagcgcgc ggggaagctg taatctgacc gttgtcccct
    12961 gccccggcgc gctttcaatt tcaacgcgcc caccggactg gcgcacaaaa ccatagacca
    13021 tcgacaaccc cagcccgctg ccgctgcccg tctgtttggt ggtaaagaat ggctcaaata
    13081 cctggctttt caccgcctgc gacataccgc tgccatgatc gatgacctcc agtgccacca
    13141 tgtcctgacg ctgcccactg ctgcgagtaa cgcgctgatt ccaggtacga attttgatga
    13201 ttcccgtctg cccttccatc gcgtcacggg cgttcatcac caggttgatg atggcatttt
    13261 ccagttggct gatgtcaatc cacgcgggcc acgccggaga ctgcgcttca atctccagcg
    13321 ccagcgttgc cggtaaggag tggcgcatta attcgcttaa ctcttccagc aatgttttca
    13381 gttcaacggc ttgcggatgt aaggactgct tgcgggaaaa cgccagcagt cgttgtgtca
    13441 gtagcgcccc acgttcggca gcttgcagcg cccgctgtat tcgcggcgcg tctggcgagt
    13501 ccggattcac cagctccagg ctgccaataa tcaccgccag cagattatta aaatcatggg
    13561 ccagcccgcc cgtcagttgc ccgaccgctt tcagtttctg gctgtgtaat agcgcctctt
    13621 ccaacccctg ccgctcagtt tgggcaatct ccatctgcgt ggtcttctct tttagcagcc
    13681 gtgaggttcg ttcgagtgac tcggtattgc gggcaaaaac gttaaaggcg cgcgccagtt
    13741 cacccaactc atcacgccgc tgtaacgccg gaaaagtgac gtccgcttcg ccgtgcgcca
    13801 ggcggctcat ggcgcgggaa atcgccgtca ggttagtgcc gaggttgcga tagatatacc
    13861 agccggcaaa cgcggtgatc accaccgcta acagggcgaa aatcatgatc accctctcgc
    13921 cggagcgcaa atcctggtaa ctttgcgcca cacgctgacc ggagagcgtc gccacctgtt
    13981 cggcgtaacg gttgatgtcg ccattgagga tcgccaccag cgctttgatg tgaaacatat
    14041 accagctgat tgccagatcg ctttgctcca gagcggcaga aagcggcgcg agtttacgca
    14101 tttcatcatt gaaatcattg agtaacgtat taaccaatgg cgtatcgctg tgggtcggca
    14161 acgcagacat taccccgacc agttgatgaa taatcgcctg cggcgcgggg gtggcaatag
    14221 cggcaacaat tagtcggttc atctcgttaa gcaaaatatc atcttgcgcg tgagtgactt
    14281 tttgcagatg ccgcagataa cttaagtttt gatacagcga acttaacagc gcgttgcgct
    14341 ccagatgccg cctttgcccg cgctccagca taccgccgac gctggtttgc agttcgttgc
    14401 tacgcgtgat gatgcgcgtc accagcccgg cttcctgctg cgctaaaggc gcgttcgcca
    14461 gttgtgcaag cgagcttttt aatgcccgct gtgtctcctg caaccgctcg gcttcgcctt
    14521 tatattccag cgctcccacc acctgcgaca atcgcaccgc cgccgttgcc acgtttgccg
    14581 tgtcgcgcgc cagattcagg ctgccggtca tatcatccac cgtttgctgc tgcaccagct
    14641 cctgcatctg gctggcatga cgaaagccca ttaccgccac gccgctgacc atcaacgtca
    14701 ccgccaccac cagcaaattc aaaagcagca atcgcccgcg cgcgctggca aaaaagtgac
    14761 gtccggggtg cgccatatca cgctccgtga agaaaaatcg caatatgaca aatatgaacg
    14821 gcttctgaca ttttgcattt actttcctgt gatggagtgc tgatatcgac aacctcgcag
    14881 gagtccacca tgaacagcac gccagttctg gagatgcgca atattgccaa agccttcggc
    14941 aaattttatg cgctcaaagg ggtggatttg acggtctacc ctggcgagat ccatgccctg
    15001 atgggggaaa acggcgcggg aaaaagcacg ctgatgaagg tgctggcggg ggcgtatacc
    15061 gccaccagcg gcgagatcct catagacggc aagccctttc acattcgcac gccaaaagat
    15121 gccttaagtg ccggtattac gctgatttat caggagatgc agctggcacc caatttgacg
    15181 gtggcagaaa atatttttct cggtagcgag ctttcccacg gcgggctggt gcagcgtaaa
    15241 gagatgctgg tgcaggcgca aagagtgatc gaccgcctcg gcgcgcagtt taacgccagc
    15301 gataaagtca tgatgctgac cattgccgag caacagcagg tcgaaatcgc ccgcgcgctg
    15361 catcgcaaca gccgcattct ggtgatggac gaacccaccg ctgccctctc ctcccgtgaa
    15421 actcaccgtc tgtttgaact gattatgcgg ctgcgcgatg aagggatggc gattatctac
    15481 atcagccacc gtatggcgga agtgtatgag ctttccgatc gcgtcagcgt gctgcgcgac
    15541 gggcaatacg ttggcagcct gacccgcgat aacctcaatg ccggggagct ggtgcggatg
    15601 atggtcggca ggccactgag cgatctgttc aataaagagc gcgatatccc gctcggcaaa
    15661 gcccgcctga atgttcacca cctgaccgac ggtggcaaag tccagccgag tagcctgctg
    15721 gtgcgttccg gcgaaattgt cggcctcgcc gggctggtag gagccggacg ttccgaactg
    15781 gcgcagttga tcttcggcgt gcggaaagcg acaggcggaa tgattgaagt cgatggtgaa
    15841 ccggtggtga tccactcccc gcgcgaagcc atcgatcttg gcattggttt tctcaccgaa
    15901 aaccgcaaag aacaaggctt attccttgaa atggcagcag ccgaaaacat caccatggca
    15961 accctggagc gcgatgccag ctggggaatg ctcaatcgca aaaaagcgca aaccatttcc
    16021 gatgacgcca tcaagttgct caacattcgc gtgcctcatg cccaggtacg cgcgggcggg
    16081 ctttccggcg gcaatcagca aaaactgttg atctcccgct gggtggcgat tggcccgcgc
    16141 attttactgc tcgatgaacc cacccgcggc gtggacgttg gcgccaaaag tgagatctac
    16201 cggatcatga acgagatggc gcgcaagggc gtggcgatcc tgatgatctc cagcgaactg
    16261 ccggagatag tgggaatgag cgatcgcgtc tatgtgatgc gcgaaggcag cattgccggt
    16321 gagttaaacg gcaaaaacat cacccaggaa aacattatga ccttagcgac tggcgtcaac
    16381 gacgcccatt cccaggcggt aacctcatga cgacctctgc aaaaacctct gccaaaaaaa
    16441 tgctgatgag cgatctgatg caaacggtcg gcatcttgcc cattttgatt ctgatcgtgg
    16501 cggtatttgg ctttatcgcg ccaaactttt ttaccgaaag caacctgctg aatattaccc
    16561 gccaggcatc gatcaacatt gttctggccg cggggatgac cttcatcatt ttgaccggcg
    16621 ggatcgatct ttccgtgggt tcgatcctcg gcaccacagc ggtggcggcg atggtcgtct
    16681 cccttagccc tgagttcgcc atgctgtcga tcccggcagc gctgatgctc ggcctgctgc
    16741 tggggctgtt caacggcgcg ctggtggcct ttgccggatt gccaccgttt atcgttacgc
    16801 ttggcaccta tacggcgctg cgcggggcgg cgtatctgct ggcggacggc accacggtta
    16861 tcaactccaa cattaatttc gagtggatag gcaacaacta tctcgggccg gtcccatggc
    16921 tggtggtgat tgcgctggcg gtgatcgtgg tgtgctggtt tattctacgc cgcaccacgc
    16981 ttggcgttca tatctacgcc gtcggcggca atatgcaggc ggcgcgtctt accggcatta
    17041 aggtctggct ggtgctgctg tttgtttatg gcatgagcgg cctgctttct gggcttggcg
    17101 ggattatgag cgcctcacgg ctgtacagcg ccaacggcaa cttaggcatg ggttacgaac
    17161 tggatgccat tgcagcggtg atcctcggcg gcaccagttt tgtcggcggc atcggcacca
    17221 tcaccggcac gctggtcggc gcgctaatta tcgccacgct caacaatggc atgaccttaa
    17281 tgggcgtctc ttacttctgg caactggtga tcaaaggggc ggtgatcatc attgcggtgc
    17341 tgatcgacaa ataccgtacc cgacaccatc aaagtgcata aacacatgag gaatacagca
    17401 tgagattgaa gcccttagtg accgcgctct gtgcaggcgc gctgctggcc gcagcaccgt
    17461 tcgcccaagc gaaagaactg aaatcgatag gcgtaacggt gggcgatctg gcgaacccgt
    17521 tcttcgtgca gatcaccaaa ggcgcggagc tggaagcacg caaactggca ggcgataacg
    17581 tcaaagtgac gctggtttcc agcggctacg atctgggtca gcaggtgtcg cagatagaca
    17641 actttatcgc cgctaacgtc gatatgatta tcctcaacgc tgccgattca aaagggatcg
    17701 gcccggcggt gaaacgggcg aaagatgccg ggatcgtagt cgtcgcggtt gacgtagcgg
    17761 cagaaggtgc tgatgccacc atcacctccg ataacaccca ggcgggcgaa atggcctgta
    17821 aatacatcac cgatcgcctg aaaggcaaag gcaatgtggt gatcatcaac ggcccgccgg
    17881 tatccgccgt gcaaaaccgc gtcgaaggat gccagactga atttaaacgc catcctgaca
    17941 tcaaagtgct ctctgacaac cagaacgcga aaggcagtcg tgaaggcggg ctggaggtaa
    18001 tgacgggact gctggcggca aatccgaaga tcgacggcgt atttgcgatt aacgatccca
    18061 ccgcaatcgg tgccgatctg gcagcaaaac aggcgcaacg taacgagttc tttattgtcg
    18121 gtgtggacgg aagcccggac gccgaagagg cgctcaagcg tgagaactcg ttgtttgtcg
    18181 ctactccggc gcaagatccc caggtgatgg cagcgaaagc ggtggaaatt ggctacgaca
    18241 ttttgcaggg caaacctgcg ccgaaagagc cggtgctgat cccggtgacg atgatcgata
    18301 aaaacaacgt cggcaattac aaaggctgga cggtgaaata agtccacgcg ccgggcggcg
    18361 cagctgcccg gtcaccgcta aggagaacgc tttcgatgaa acgctccgct ataaacgaca
    18421 ttctcggcca tacacggcaa ttcttttcgc aacatgacgt acatctgccg ccctttgcca
    18481 gtttctcgcc tgctcaatgg cagcagctcg acaccgccgc gtgggaggag gtttttgatc
    18541 ttaaactcgg ctgggatgtt accgcttttg gtcgcaacaa ctttgcagca cacgggctga
    18601 cgctgtttac cctacgtaac ggctcagcaa aaggaatgcc gtatgtgaaa tgttacgccg
    18661 agaagatcat gcatgtgcgc gatgcgcagg tcacgccaat gcattttcac tggcgtaagc
    18721 gtgaggacat tatcaatcgc ggcggcggta atttaattgt tgaactgtgg aatgccgata
    18781 gtaacgagca aacggctgat agtgacatta cggtggtgat agacggctgc cgccagaaac
    18841 atactgcagg cactcagttg cgcctgtctc ccggcgaaag tatctgcctg ccgcccggcc
    18901 tgtatcacag cttttgggcg gaaaccggct ttggcgatgt tctggttggc gaagtctctt
    18961 ctgtcaatga cgacgaccac gataaccact ttttacagcc actggaccgc tacaacctga
    19021 ttgacgaaga cgaacctgca cagttggtat tgtgtaacga ataccgccag ttccgctgaa
    19081 taaggataac gttatgccat tgatttgtct cgctgaaggg ctggcacatg cccgcgaaca
    19141 tcactattcg ttaggtgcgt ttaatgtgct ggactcgcac tttttacgcg ccttgtttgc
    19201 tgctgccaga caagaacgtt cgccctttat tatcaacatt gccgaagtac attttaaata
    19261 tatctcgctg gattccctgg tcgagacagt gaaatttgaa gcggcgcgtc atgccattcc
    19321 ggtggtgtta aatctcgatc acggactgca ttttgaatcg gtggtgcaag cgttgcggct
    19381 gggattcagc tcggtaatgt ttgatggctc cacgctgggg tatgaagaaa atgtccgcca
    19441 gacgcgggaa gtggtgaaaa tgtgccatgc cgttggcgta tcggtagaag ctgaactggg
    19501 cgcggtgggt agcgacgaag gcggtgcgct ctatggtcat gccgatgaat ccctgtttac
    19561 cgatccgcaa ctggcgcgtg attttgtcga ccgtaccggc attgacgcgt tggccgtggc
    19621 gattggcaat gcacacggca aatatcgcgg cgaaccgaaa ctcgatttcc cgcgcctcga
    19681 cgctatccgc cagcaggctg ctatcccgct ggtattacac ggcggctcag ggattagcga
    19741 tgccgatttc cgccgcgcca tcgaactggg tatccacaaa atcaatttct acactggcat
    19801 gtcgcaagcg gcgctggctg ccgttgaggc ccgaatggcg caccgtcagc cgatgtatga
    19861 cgagtttgcc gagttactgc tggcagtgga agccgccatc agcgatacgg ttgcacaaca
    19921 gatgcgtatt tttggcagtg cggggcaggc atgatggagc gtaaagggat tatcgccgca
    19981 ggcaatatgt tggttgacca cgtccatcag attatccagt ggccagagcg cggctggctg
    20041 gcggagatca cccacagcga acgctccacc ggcggtgcgc cgcttaacgt cctgctgacg
    20101 ctggcgaaaa tgcgcaccgg gctgccgtta caggcggttg ggctaatcgg tcaggacagc
    20161 gacggcgatt acatcatggc gatgcttgag caatatcacg tcaatcgtca gcatgtgcag
    20221 cgcactacct ttgccccgac gtcgatgtcg caggtgatga cagacccaag cgggcagcgg
    20281 acgtttttcc attcgccggg tgcaaacaga cttttggatc ttccggcgtt cgatcagctc
    20341 gataacacca tgaagatttt ccatctcggc tatttactgt tgctcgacag cctggatatg
    20401 ccggacgatg tttacggcac ccgtagcgca cgcctgctgg cgcaaatgcg tgatactggc
    20461 tttgaaacct cactcgacct ggtctcccgt aagggcgatc cgcgttatca gccgctggtg
    20521 ctgcccgcgt tgcgccatct ggattatctg gtaattaacg aactggaggc cggggagttt
    20581 agcgggctgg aaattcggtt acccaacggc gaaccgcata tcgcgcatat tgctgccgcc
    20641 gcgcgtgaac tgttagatgc gggcgtgcgc cagcgggtgg tgatccactg cccggaaggg
    20701 gcgtggggag agaccccaga acagggcagc gtgtggatcc cttcgcaaaa gttagaacaa
    20761 cgggaaatta tcggcagcgt cggcgcgggc gatgcatttt gtgccggatt tttatatggc
    20821 tgccatgaac gctggacgct taccgagagc atcaaactgg cacacgcctg cgcgcgggca
    20881 agcctgttat gcgccaatgc catcgacggg gcgaaaacgc tggaagagct gcaaacagaa
    20941 atgtcggatt aatgcacgtc ggcagcgaag acatacccca gaccgcgcag agttttaatc
    21001 aacatcggct gatgcgggtt gagttcgatt ttgcgtcgta agcgcatgat taatacgtcg
    21061 atggtgcgat caaacacctc cacgctgtcg ctgtgggtga acgccagcaa ctgctcgcgg
    21121 ctcaataccc gacgcgcgtt ttgcgccagt gccagtaaca agccatattc gccctgagtt
    21181 aactcgacat gttgctgaag cggattgtgc aactggcagc gcatggtatc gagataccag
    21241 ccattgaatg acagcccggc gcttttctcg tcattaaccg ccagcgcacc aacccgtcgc
    21301 aacaccgctt tcagtcgtgc gaccaccacc cgcggggtga acggtttgca gatgtaatca
    21361 tccgcgccca tttccagccc gaccaccaca tcagactcac tgcccatgcc ggtcaacatc
    21421 accaccggca atgtcggacg cagtttctgg atttgctgca ataccctcag gccgttggta
    21481 tcgggtagca tcatatccag caccaccagc gcaatgtcag gatcgccctc aatacgcaat
    21541 atggcctcct gtccggtatg gcagactgaa accgtaaaga catgttcact taacacgtcc
    21601 tgcaacagtg cgcagatagc cgtgtcatca tccaccacca gcacgacggg tttcatggtt
    21661 acactccgca tcattcatct gattaatcat tgtgcgtact tcttgtcgaa gtgaaagctg
    21721 aaagtaatat aaataataaa aatataagtc aggtcacatt attgatgata gtaataaata
    21781 tgccgtaatt ttccctcttc agcacaaatg cctaataaaa tggatcgttc cgctcacact
    21841 tccacttatt aaagtcgcaa tttcccaatt atgataacgc cagctttaaa tgacgtattt
    21901 tgcatggatt acatatttac gacataaatg ataaatggag atttatatga ataactctca
    21961 gttattccgt ttgagccgga ttgttattgc gttaactgcc gccagcggca tgatggtaaa
    22021 taccgcttac gcgaaagagg aagcgaaagc cgccactcaa tatacccaac aggttaatca
    22081 gaattacgcc aaatcattac cgtttagcga tcgtcaggat tttgacgatg cccagcgtgg
    22141 atttatcgcc ccgctgctgg atgaaggtat tctgcgtgat gcgaacggta aagtttacta
    22201 ccgcgcggac gattacaaat ttgatattaa tgccgcagcg ccggaaaccg taaaccccag
    22261 cctgtggcgt cagtcgcaaa tcaacggtat ttctggcctg ttcaaagtca ccgataaaat
    22321 gtatcaggtg cgcggccagg atatctctaa cattacgttc gttgagggcg agaaaggcat
    22381 tattgttatt gacccactgg tgacgccacc tgcagcaaaa gccgcacttg acctttactt
    22441 ccagcatcgt ccgcaaaaac cgattgttgc cgttatctac actcacagcc acaccgacca
    22501 ctatggtggc gtgaaaggca ttatctctga agccgatgtt aaatccggca aagttcaggt
    22561 gattgcccct gctggcttta tggacgaagc catcagcgaa aacgtgctgg cgggtaacat
    22621 catgagccgc cgtgcgctct actcttacgg tctgttactg ccgcacaacg cgcaaggcaa
    22681 cgtgggtaat ggccttggcg tgacgctggc aacgggcgac ccgagcatta ttgcaccgac
    22741 taaaactatc gtcagaactg gcgagaagat gattatcgac ggcctggagt ttgacttcct
    22801 gatgacccca ggtagcgaag cgccagccga aatgcacttc tatattccgg ccctgaaagc
    22861 cctgtgtacc gccgagaacg ccacgcatac tctgcacaac ttctacactc tgcgcggcgc
    22921 gaaaacccgc gataccagca agtggaccga gtacctgaac gaaacgctgg atatgtgggg
    22981 taacgacgcg gaagtgctgt ttatgccgca cacctggccg gtctggggca ataagcatat
    23041 caatgattat attggtaaat atcgcgatac catcaagtat attcacgacc agaccctgca
    23101 cctggcgaac cagggctaca ccatgaatga aatcggcgac atgattaagc tgccgcctgc
    23161 gcttgccaat aactgggcca gccgcggcta ttacggttct gtcagccaca acgcccgcgc
    23221 ggtgtataac ttctatcttg gctattacga cggtaacccg gctaacctgc atccgtatgg
    23281 tcaggtagag atgggcaaac gttacgtgaa agcgctgggc ggttctgccc gtgtcatcaa
    23341 cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca
    23401 ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga ataactttga
    23461 acagctgggc tatcaggccg agtccgccac atggcgcggt ttctacctga ccggcgcgaa
    23521 agagctgcgc gaaggggtgc ataagttcag ccacggcacc accggttccc cggacaccat
    23581 tcgcggaatg tcggtcgaaa tgctgttcga ctttatggcc gttcgcctcg atagcgcgaa
    23641 agctgcgggt aaaaatatca gcctgaactt caatatgagc aacggcgata acctcaacct
    23701 gacgctgaac gatagcgtgc ttaactaccg taaaacactg caaccgcaag ctgacgcctc
    23761 tttctacatc agccgtgaag atctgcacgc cgtgctgacc ggacaagcca aaatggcgga
    23821 tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg aagaaattat
    23881 cgcctgtctg gataatttcg atttgtgggt gaatatcgta accccaaatt aatgtcactc
    23941 gctttt
//
LOCUS       DAZUGK010000055        23558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZUGK010000055 DAZUGK010000000
VERSION     DAZUGK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.055"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..201
                     /locus_tag="ACHY5W_001927"
     CDS             <1..201
                     /locus_tag="ACHY5W_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFS4446831.1"
                     /translation="RREFAIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLR
                     DQIALRVTEAGADDEEELQQEE"
     gene            201..2303
                     /locus_tag="ACHY5W_001928"
     CDS             201..2303
                     /locus_tag="ACHY5W_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4446832.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLAAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEAPVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPTPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            2300..2512
                     /locus_tag="ACHY5W_001929"
     CDS             2300..2512
                     /locus_tag="ACHY5W_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446833.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2512..4020
                     /locus_tag="ACHY5W_001930"
     CDS             2512..4020
                     /locus_tag="ACHY5W_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4446834.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            3965..5992
                     /locus_tag="ACHY5W_001931"
     CDS             3965..5992
                     /locus_tag="ACHY5W_001931"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFS4446835.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6079..6402
                     /locus_tag="ACHY5W_001932"
     CDS             6079..6402
                     /locus_tag="ACHY5W_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFS4446836.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYMKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6395..6670
                     /locus_tag="ACHY5W_001933"
     CDS             6395..6670
                     /locus_tag="ACHY5W_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS4446837.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            6682..7260
                     /locus_tag="ACHY5W_001934"
     CDS             6682..7260
                     /locus_tag="ACHY5W_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4446838.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7257..7658
                     /locus_tag="ACHY5W_001935"
     CDS             7257..7658
                     /locus_tag="ACHY5W_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4446839.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     ILIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            7669..8412
                     /locus_tag="ACHY5W_001936"
     CDS             7669..8412
                     /locus_tag="ACHY5W_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4446840.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            8473..8859
                     /locus_tag="ACHY5W_001937"
     CDS             8473..8859
                     /locus_tag="ACHY5W_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4446841.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGTIDNAGDDEHEALTPEKS"
     gene            8868..9197
                     /locus_tag="ACHY5W_001938"
     CDS             8868..9197
                     /locus_tag="ACHY5W_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4446842.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9169..12234
                     /locus_tag="ACHY5W_001939"
     CDS             9169..12234
                     /locus_tag="ACHY5W_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4446843.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEAEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELVRGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     ISQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNES
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQLLALQQRISDLDGKKLTTDEKSVLA
                     RKDELIQALMLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKNQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12234..12563
                     /locus_tag="ACHY5W_001940"
     CDS             12234..12563
                     /locus_tag="ACHY5W_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4446844.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12573..13271
                     /locus_tag="ACHY5W_001941"
     CDS             12573..13271
                     /locus_tag="ACHY5W_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4446845.1"
                     /translation="MQDIPQETHHETTRLTQSAQMVLWEIDLTEVGGERYFFCNEQNE
                     KSEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13276..14019
                     /locus_tag="ACHY5W_001942"
     CDS             13276..14019
                     /locus_tag="ACHY5W_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4446846.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            13917..14564
                     /locus_tag="ACHY5W_001943"
     CDS             13917..14564
                     /locus_tag="ACHY5W_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4446847.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMVLGGVAQMLAPKARTPTATSTD
                     NGKQNTYFSSLDNMVAQGNSLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            14625..18023
                     /locus_tag="ACHY5W_001944"
     CDS             14625..18023
                     /locus_tag="ACHY5W_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS4446848.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDNEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSATIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGNYRLTVRAVNAWRQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            18090..18689
                     /locus_tag="ACHY5W_001945"
     CDS             18090..18689
                     /locus_tag="ACHY5W_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4446849.1"
                     /translation="MRKLYATILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            18754..22107
                     /gene="stfR"
                     /locus_tag="ACHY5W_001946"
     CDS             18754..22107
                     /gene="stfR"
                     /locus_tag="ACHY5W_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS4446850.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDTRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASNASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNAASSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASVSATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRSWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASA
                     VSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22107..22682
                     /locus_tag="ACHY5W_001947"
     CDS             22107..22682
                     /locus_tag="ACHY5W_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4446851.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(22780..23370)
                     /locus_tag="ACHY5W_001948"
     CDS             complement(22780..23370)
                     /locus_tag="ACHY5W_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4446852.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         5735 a         5605 c         7059 g         5159 t
ORIGIN      
        1 agacgggagt ttgccatctg ggcaaaagcg gtcgtgcagg tgctggagac attaccggat
       61 attctggaac gtgactgcgg cctgcagcct gccgctgtga gccgtgttca gtccattatt
      121 gatgatctgc gcgatcagat agccctgcgg gtgaccgaag caggtgcgga tgatgaggag
      181 gaattacagc aggaggagta atgctgaatc aggaaaccgc aaaggcagca cgaaccgatt
      241 caggttatat ccttcgcgca ccgagacgaa tgcgggttgc tgatgccgtt gctcagtata
      301 tgcgggtgcc catgggggcc gggaactcag tcccgtggga tccgctggtg gcaccgtatg
      361 ttattgagcc gatgaactgc ctggcctcgc gtgaatacga cgcagtgata tttgttggcc
      421 cggcacgaac tggcaagact atcggcctga ttgacggctg ggtgatttac aacgtgattt
      481 gcgatcctgc tgatatgctg atcattcaga tgacggagga aaaagcccgc gaacactcca
      541 aaaaacgact tgccagaacg tttcgcgtca gcccggaagt ggtcagtcgc ctgagtccga
      601 acaaaaatga caacaacgtt tatgacagaa cattccttgc tggcaactac ctgaaaatcg
      661 gctggccgtc agtcaatatc atgtcctcat cagattataa atgcgtggcg ctgacggatt
      721 atgaccgttt tccggaagat attgatggcg agggggatgc cttctctctt gcctcaaaac
      781 gtaccaccac atttatgtcc agtggtatga cgctggtgga gagttccccc ggcagggatg
      841 tgaaggatgt gaaatggcga cggacttcac cgcatgaggc tccaccaacc acggggatac
      901 tgtcgctcta taaccgtggc gatcgccgtc gctggtactg gccctgtcca cactgtggtg
      961 agtattttca gccctgcggc gatgtggttg ctggtttccg tgatattgcc gatcccgtgc
     1021 tggcaagtga ggcggcttat attcagtgtc cttcctgttc aggacggatt atgcctgaac
     1081 aaaaacgtga gctgaacgga cgtggggtct ggttgcggga tggtgaatcc atcaatgcgg
     1141 atggcagtcg ttatggtgat ccccgacgct cacgtattgc gtcattctgg atggagggtc
     1201 cggcagctgc ttaccagaca ctctcgcaac tcgtttacaa actgcttgct gcagaacagg
     1261 aatacgagac aaccggaagt gaagaaacac tcaagacggt tatcaatacc gactggggat
     1321 taccttatct tccccgtgcc agcatggagc aacgaaaaag tgaactgctt gagcagcggg
     1381 cagagccagt tccttcccgc agtgtgccgg atggcgttaa tttcctggtg gcgacagttg
     1441 atgtgcaggc gggacgtcat cgccgttttg tggttcaggt aacgggctat ggcagccgtg
     1501 gcgaacgctg gattattgat cgttacaaca tcacgcagtc attgcgcggt gacagcgacg
     1561 gggagagcca gcgaattgat ccggccagct atccggaaga ctgggatgtc ctgctgacgg
     1621 atgtttttca taaaagctgg ccgctggcct ccgatccttc tcaacaaatg cgactgatgg
     1681 caatggcggt ggactccggc ggtgaagacg gagtcactga taatgcctat aaattctggc
     1741 gtcgttgccg tcgtgatggc cttggtaaac gtatttacct gtttaagggc gacagcatcc
     1801 ggcgcgcaaa actgatcacc cgtacattcc ctgataacac cggacgaacg ggccgacggg
     1861 cgcaggccgc aggtgatgta ccgctctggc ttcttcagac ggatgccctg aaagaccggg
     1921 tgaataacgc gttatggcgt gactcgccag gtcccggcta tgtgcatttc cctgactggc
     1981 tggggagctg gttttacgac gaactgacgt atgaagagcg gagcagtgac gggaaatgga
     2041 gtaagccggg tcgcggtgcc aacgaagctt ttgacctgat ggtgtatgcc gaggctccgg
     2101 tcattctgca tggatacgaa aagatccgct ggccggatgc accggagtgg gcgagccggg
     2161 aaacctggct ggagtgtgtc ccggacagta ccgaaccgtc acccacaccg gaaccggtat
     2221 ccacgcctgt taaaaaacaa aaacggaaga aaacagtaac tgacgatgtt aacccctggc
     2281 tgacttccgg aggatggtta tgaaccagaa tgatatcgaa gccatgattc agcgttatac
     2341 ggaagctgaa atggcggtgc tggacggaaa atccgtcacc tttaatggtc agcagatgac
     2401 catggaaaac ttatctgaga tccggcaggg acggcaggag tgggagcgcc gccttgcggc
     2461 tctgattaca cgacgacggg ggcatcccgg gtaccggctt gcgaggttct gatggcaatt
     2521 cttgatgatg tgattggcgt tttttcacca ggatggaaag cggcaaggct gcgttcccgt
     2581 gcggtgatcc aggcttatga ggccgtaaaa acgacgcgga cacacaaagc ccggcgggaa
     2641 aaccgaactg ccgaccagtt aagccagtac ggggccgtgt cgttacgtga gcaggcccgt
     2701 taccttgata acaaccacga tctggtcatt ggtgtatttg acaagctgga agaacgggtg
     2761 gtggggaaaa acgggattat tgtcgagcca catccggtat tacgcaatgg ggccattgcc
     2821 cgtgatctgg cagcggagat acgcacccga tggagtgaat ggtctgtcag tccggaagtc
     2881 accgggcagt ttacccgtcc gatgctggaa cgtctgatgt tgcgtacctg gctgcgcgat
     2941 ggtgaggtgt ttgcccagat ggtttccggg cgcataaaca gcctgacgcc ttctgccggt
     3001 gttcatttct ggctggaggc gctcgagccg gactttattc ccatgaccag tgatgagagc
     3061 aacaggctga atcagggcgt gtttgttgat gactgggggc gtcccgaaaa atatctggtg
     3121 tataaaagcc gtcccgtatc cggacggcag atggaaacca aagaagtgga tgcagagcga
     3181 atgctgcatc ttaaatttgt tcgccgtctg caccagatgc gcgggacgtc tttgttgtcc
     3241 ggtgtgctga tccgcctcag tgccctgaaa gagtatgaag attctgagct gactgcagca
     3301 aggatcgccg ctgctctggg gatgtacatc cggaaaggcg acgggcagag ctatgaaccg
     3361 gatggtaatg gcagcaagga taaggaacgc gagcttacca ttcagccagg cattatttac
     3421 gacgatctga aacccggcga agaaatcgga atggtgaagt cggatcgccc caatcctaat
     3481 cttgaaactt ttcgtaatgg tcagttgcgt gccgtggcgg cgggcagtcg tctgagtttt
     3541 tccagtacag cgcgcaacta taacggcact tacagcgccc agcgtcagga gctggttgaa
     3601 tccactgatg gctacctgat cctgcaggac tggtttattg gtgccgtcac ccgcccgatg
     3661 tatcgtgcct ggctgaaaca ggctgtggca tccggtgtta tcaggctacc ccgcgatctt
     3721 gatcgttctt cactgtatac cgcggtgtat tccggaccgg tgatgccgtg gattgaccct
     3781 gttaaggagg ctgaggcctg gaaaattcag attcgtggtg gagcggcgac agaatcagac
     3841 tgggtacgtg caggtggtcg taatccggat gatgtcaaac gtcggcgcaa ggccgaaatt
     3901 gatgaaaacc gcaagctgga tctggtattt gataccgatc cggccagtga taaaggaggc
     3961 agcagtgccg caacgaaacg acaggagccg cagcacaccg acgaccagtc cgaagaataa
     4021 ttcctggttc aggatgcagg caggtcacca gagtgacgcg gatatttata tttatgacga
     4081 gattggtttc tggggtgtta cggcgaagca gtttatcagt gatctgaatg cactgggcga
     4141 tatcacccac attaatctcc atattaattc accgggtggc gatgtctttg aaggcatcgc
     4201 catttttaat gcactgaaaa cacatggtgc gtccattacc gtttatgtcg acggtgtggc
     4261 ggcgtcaatg gcgtcggtca ttgcgatggt gggaaacccg gtcattatgc cggaaaactc
     4321 cttcatgatg attcataaac catttggctt tacgggcggt gatgcggagg acatgcgcac
     4381 ctatgccgac ctgctcgata aagttgaggc ggttctgtta cccgcttatg cacagaaaac
     4441 cgggaaaacc accgatgaaa ttgctgccat gctggcggat gagacctgga tgtccggtgc
     4501 cgaatgtctg gcacagggat ttgctgatca ggtgacacca gccgttaagg caatggcatg
     4561 tattcagtca aaacgtacag aggaatttaa aaagatgccg gaatccattc gaaatatgat
     4621 tactccgcca cgcaacagtg ctccacgcgt acaggatgat ggacctgcag cctcccggac
     4681 gccagtgcag gcagcagcac ccgtggtgga tgaaaacagc atccgtgcgc aggtcctggc
     4741 agagcaaaaa gcgcgtgtaa acggtattaa tgatctgttt gccatgtttg gtgggcgtta
     4801 tcagtcgctg caggctcagt gtcttgccga tcctgaatgt tcgctggagc aggcccgcga
     4861 aaagctgttg aacgagatgg ggcgcgagtc cacgccatcc aataaaaata ccccggctca
     4921 tatttatgcc ggtaacggta attttgtggg ggacgggatc cgccaggcgc tgatggcgcg
     4981 cgccgggttt gaaaaaaccg aacgtgataa tgtctacaac gggatgaccc tgcgtgaata
     5041 tgcccgtatg tcactgactg aacgtggtat tggtgtttcc agttataacc cgatgcagat
     5101 ggtcggtgcg gcgttcacac acagtacgtc tgacttcggt aatattctgc tggatgttgc
     5161 gaacaaagcc attctgcagg gctgggaaga tgcccctgaa acctatgaac agtggacgcg
     5221 gaaaggtcag ttgtctgatt ttaaaattgc ccatcgtgtg ggtatggggg gcttcagtgc
     5281 tctgcgtcag gtgcgtgaag gggcagaata taaatacgtc accaccggag ataaacaggc
     5341 cactattgca ctggcgacct atggcgagct gttcagtatc acccgtcagg ccattatcaa
     5401 tgatgatctg aatatgctga ccgatgtccc gatgaagctg ggccgtgcgg cgaaatccac
     5461 tattgccgat ctggtttatg ccattctgac gtctaacccg aaaatctcca cagataatgt
     5521 aagtctgttc gataaagcga aacatgcaaa cgtactggag agcgcggcaa tggacgtggc
     5581 atcgctggat aaagcccgcc agttgatgcg cgttcagaaa gagggtgagc gtcatctgaa
     5641 tattcgtcct gcgttcgtac tggtaccgac ggcgatggag tctgttgcta accaggtcat
     5701 tcgctcctca agtgtcaagg gggctgacat taacgccggt attattaacc cggtgaaaga
     5761 ttttgcgacc gttattgcag agcctcgtct tgatgataac agccagacca ccttctacct
     5821 ggctgcgtcc aaaggctccg atacgattga agtggcttat ctcaacggtg tggatacgcc
     5881 atatattgat cagatggagg gcttcagtgt ggatggcgtg acaacgaaag tacgtattga
     5941 cgctggtgtt gcgccagttg atcaccgcgg cctagtgaaa tgtacggcgt aaacgtcgca
     6001 gacaacaact ctgatggccc gtaagggctt tttttgtacc tgaaatcagc ccctgaacgg
     6061 ggctgtgcgg agacagttat ggcaaagaat tttgtagaag aaggaaaaac ggtggcgatt
     6121 gttgccagtg cagccatcag cagcggagat ctggtgcagg tgggtgatgt ttttgcggtg
     6181 gcgctgaccg atattccaca gggagaaaca ggcgacggca taaccgaagg tgtgtttatg
     6241 ctgcctaagc tgaaaacgga tgacatgaaa acgggtaaga aggtttatat gaagtcagga
     6301 aaagttcagc tgactaacag cggctctgat ccgctggtcg gggttgtctg ggcagatgcc
     6361 ggaaccagtg cagaagaagt gccggtaaaa ctcaatgtct gatccctttt cccggctggc
     6421 agcgcgtatg gatgcgatca cggtcagaaa gatgggaaag acagcctcga ttaatgatgt
     6481 cgatatgact gtgatcccgg gagaaacact ggcagagctg aatgctctgt ccggacctgc
     6541 ggtctctctg gtggtgtttt cttcgggata ccgcccacgg cgcggggatc gcgttgttta
     6601 tgacggacaa caatggacgg tcacacggca tgaacgcttt aacggtaagc caatgatctt
     6661 tattgagtaa agaggtgtgg gatgaagggg cttgagaatg ccatccgcaa tctgaacagc
     6721 cttgataccc gtatggtgcc acaggccagc gcatgggcga taaaccgtgt ggcacagaaa
     6781 gcggtctcgg ttgccacccg gcaggttgcc gggaataccg ttgcgggaga taaccaggtg
     6841 aaagggatcc ccctgaaact ggtacgtcag cgtgtccggg tgtttaaagc cagtccgtca
     6901 ggaaaaatga cggccaggat ccgcgttaac cgaggcaatc tgcccgccat taagctgggg
     6961 acagcccggg tcagactggc ccggcgtggt ggaaaactgc agtaccgtgg cagtgtgctg
     7021 aaggtgggta aatatctttt ccgggatgcg tttattcagc aactggcgaa tggtcgctgg
     7081 catgtgatgc gacgtattga tggcaaaaat cgttacccca ttgatgtggt gaaaatcccg
     7141 ctgtccggac cgctgacaca ggcatttgaa gatgcccgcg accacatcat tgctgcggaa
     7201 atgccgaaac agctggggta tgcactgaaa caacaactga ggttatggct gacccgatga
     7261 accgacatac acaaatccgc caggccgtac tggcacgcct tcgggaacag tgtggagaca
     7321 gcgccacgtt ttttgacggg cttccggcat ttattgatgc gcaggaactg cctgccgtgg
     7381 cggtgtggct gagtgatgct cagtacaccg gaaaaatgac ggatgaagat gactggcagg
     7441 ctgttctgca tattgccgtc ttcatccggg cacaggcacc ggattcagag ctggatatgt
     7501 ggatggagag caccattttc cctgccctga atgatgtacc ggcactttcc ggactcatcg
     7561 acatcctgat cccactcggt tttaactatc aacgtgataa tgagatggcc acctgggcga
     7621 tggcggaaat cacgtaccag atcacgtaca cgaattaagg aggtggtaat gaccacacca
     7681 aatccactgg caaagacgaa aggtgcgggg acgacgttct ggatgtatac cggcaacggc
     7741 gatgcgtttg cgaacccttt gtcggacact gactggctgc gtcttgcgat ggtgaaggat
     7801 ctgcaacctg gcgaaatgac cgctgatgca gaagatgaca cttatctcga tgatgaagat
     7861 gcagactgga aaacgacaac ccaggggcag aaatccgtcg gtgatacttc ggcgacgctg
     7921 gcctggcgtc cgggtgacag cgggcagaaa aaactggttc agttgttcga ctccggtgaa
     7981 gtctgcgcgt ttcgtatcaa atatcccaac ggtactgttg atgttttccg tggctggctg
     8041 agttcactgg gtaaaaccat tgcctcaaaa gacgtgatga cccgcacagt gaaaatcagc
     8101 ggtgtggggc gtccgtatct ggcagaagaa ggcactgaaa ccgtgggcgt taccgggctg
     8161 acggtggcac cggcatctgc cagtgtaaaa gtgggagcaa ccaccacgct gacctttaca
     8221 gtaaaacctg acggagccag tgacaaagcg atcagtgtgc attcgacaga tccacagact
     8281 gccacggtga ccctgaacgg gcttgtggcc acggtgaaag gcgtgaagca gggcagtgtc
     8341 agcattgtgg gcatgacttc tgacggcgat tttgtggcag tggctacggt ggctgtcagc
     8401 gccgcaggtt aacaggacga tactcatcat ttgccccgat tatccggggc ttttttgcag
     8461 gtggagaaca tgatgtttct gaaacagggc acgtttaatt atgaaaagca gtccgtggtg
     8521 ctcagtgagc tgtccgggct gcagagaatt gaatatctgg cgtttgttca gcagcgaacg
     8581 gcaaagtttg atgccgaaga gggagaactg ccggaggctg aacgacagat tgcttttctg
     8641 cggatgggga tggatatcaa tgcctggctg gtttcccgct cactgtggaa tgcggatcag
     8701 tctaaggatg tagagacgct ttgcgcatcc gttattacaa catggtcgta tgatgccctg
     8761 ggtgcggggg cggagatggt tctgtcgctg agcggtatgg ggaccattga taatgccggg
     8821 gatgatgagc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg cagtttgtca
     8881 tgcggcttgc ccgggagttc cgacgggcag actggcggcg gatgctgtcg gaaatgtcgg
     8941 ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt gatgtgtgga
     9001 tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc ggtagcagtg
     9061 atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg gagcgaacgg
     9121 acgaagaact gatgcatctt ggggaaggta tttccggagg tgtgcgttat ggaccagata
     9181 gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga aattccccgt
     9241 atcaaaaacc ttctgaatgg tgcagccagc gatgcagaac ggtcttctgc ccgtatgcag
     9301 cgttttatgg agcgtcagac tcaggccgcc cggcagacaa tgcaggcggc ttcttcggct
     9361 gcaactgccg catccgtcca tgcgcagacg gtggagaaga gcgcacaggc tcatgaacgc
     9421 atggcccgcg aggtggagca aacccgccag cgtatggagg cactgagcca gaaaatgcgc
     9481 gaggaacagg cgcaggccat ggctctggcg gaggctcagg ataaagcggc tgccgcgttt
     9541 tatcgtcaga ttgacagtgt gaaacaggcc agtgcggggc tgcaggaatt acagcgtatt
     9601 cagcagcaga tccgacaggc tagaaacagt ggcgggattg gtcagcagga ttatctggcg
     9661 ctgatttctg aggttacggc gaaaacccgt gttcttacgc aggctgaggc agaggctacc
     9721 cgacagaaag tggcgtttat ccgtcagctt aaagagcagg caacccgcca gaatctttct
     9781 tcttctgagt tgcttcgtgc taaggctgcc cagctggggg taagcagtgc tgcagaagtg
     9841 tatatccgca aaatggagca ggcaggaaaa gccacgcatt cgctgggtct gaaaagtgca
     9901 gcggcccgcc aggagatagg cgttctgata ggtgaactgg ttcgcggcaa tttaggtgcg
     9961 ctgaggggat ccgggataac gctggctaac cgtgccggat ggatagacac actgatgtca
    10021 ccgaaaggca tgatgctggg cggggttatt ggcggtattg ccgcggccgt ctatggtctg
    10081 ggtaaagcct ggtatgatgg tcagaaggag ggggaagaat ttaaccgcca gttgtcgctg
    10141 acggggcatt atgccggagt cactgccggg cagctgtgga cgctcagtcg tgctatttcc
    10201 gggaatggta tcacgcaaca tgctgcagcc ggtgcgctgg ctcaggtggt ggggagtggc
    10261 gcatttcgtg gaaacgatat cggtatggtg gcgagagctg ccgcacagat ggagcgatcg
    10321 gttggccagt cggtcagcga taccataagt cagtttaagc ggctgaagga tgatcctgta
    10381 aatgccgcga aggctctgga caatgagctg cattttctta ctgccactca gcttgagcag
    10441 atacgtgtcc ttggggatca gggacggtcc agtgatgctg cacggatagc catgtctgca
    10501 ctggcagagg aaaccggtcg gcgtactgcg gatattgata ataacctcaa tgcgcttggc
    10561 agtacgctga agtatctgtc tgatttgtgg agtcgtttct gggatgcggc catgaatatt
    10621 ggtcgtgaag actcgctgga tgaacagatt gccgctttac aggagaaagt gtcgcgggcg
    10681 aaaagactcc cctggacggc atcatcttct caggttgaat acgatcagca gcgtcttaac
    10741 gatcttcagg agaaaaaacg ccagaaggat ttgcaggatg caaaagagca ggcagagcgg
    10801 aattatcagg agcaacagaa acgacgtaat gctgaaaatg ctgcactgaa ccggatgaat
    10861 gaatcggaag cagcacgaca tcagcgtgaa attgcgcgta ttaattccat gcagtacgcc
    10921 gatcaggctg tcagggatgc agcgatacaa cgtgaaaatg aacgttacga gaaagccctg
    10981 gcatccggta agaaaaaaac acgcgaaacc cgtaatgatg aggccacccg gttattgctg
    11041 cagtacagtc agcaacaggc acaggtggaa ggacagattg ctgctgcaag acagtcagca
    11101 ggcattgcca ctgaaaagat gacagaagcg cataaacagc ttctggctct gcagcagcgc
    11161 atcagcgatc tggacgggaa aaaactgacg acagatgaaa agagtgtgct ggcccgtaaa
    11221 gatgaactga ttcaggcact gatgctgctg gatgtaaaac agcaggagct tcagaaacag
    11281 acggcactca acgatctgaa gaaaaaaaca attcagctga ccagtcaact ggctgaagaa
    11341 gagcgcgctc agcgtcagca acatgacctg gatatcgcca cggtgggtat gggtgatcag
    11401 cagcggcagc gatatcaggt acaactgagt cttcgccaga aaaaccagca acagctggag
    11461 cagttgaggc gggatagtga gcagaaaggg acatataaca cggatgacta cagaaaggcc
    11521 gagcaggcgc tgacggagag cctgaaccga caactgaatg agaatcgccg ttactggcaa
    11581 cagcttgaag ttgtgcaggg taactggaaa aacggagtcc tgcgtgcatt tcaggatttt
    11641 accgtggatg cagacaatac ggcaggaaca gcagaacagg tgttctcgtc agccttcagc
    11701 aacatgggaa atggcctggc aacttttgtc actaccggca aactcaactt caaatccttc
    11761 acctcttcgg tgctgtcaga tatggcgaaa atcctggcgc aggcaaccat gatgaaatcg
    11821 ataaaaggga ttggcagtgt actgggattt gatctcagca gcctttccct gaatgccaat
    11881 ggggggattt atcagtctgc tgatttgagt cgttacagtg gcacggtggt taaccgtccg
    11941 acgttttttg cttttgcaaa aggcgcgggt gtgatggggg aagctggacc tgaagccatt
    12001 ctgccactgc gtcgtggtgc tgacggtaag ctgggggttg tggcggatat tggtggttca
    12061 ggtatggcga tgtttgcccc gcagtacaac atcgagatca ataacgatgg cacgaacggg
    12121 cagataggtc cggctgccct gaaggtggtt tatgaccttg ggaaaaaagc ggcagcggat
    12181 tttatgcaac agcaggcccg tgatggtggt cggttaagtg gagcatatcg gtaatggaga
    12241 cgtttcactg gaaagtgcgc ccggatatga atgtggtatc agagccgaaa gtggtgacag
    12301 tgaagctggg cgatggttat gaacagcgtc gtgcggcggg actgaataac cagttgtcga
    12361 tttacagcgt gacgatacgt gttcgtaaat gtgaacaccc atctttaaaa gcctttctgg
    12421 aacggcacgg tggcgtccgc gcatttcagt ggacgccacc ttatgactgg aagccgatca
    12481 gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt gtgggtaacc ataacggcag
    12541 attttgaaca ggtcgtggca taggagtctc tgatgcagga tattccacag gaaacacatc
    12601 atgagacgac acgcctcact cagtcagccc agatggtgct ctgggaaatc gatctgacag
    12661 aggtcggtgg agaacgttat tttttctgta atgagcagaa cgaaaaaagt gagccggtca
    12721 cctggcaggg gcgacagtat caggcgtatc ccattcaggg gacgggattt gaactgaacg
    12781 gcaagggcag tgctgcccgt ccgacactga cggtttctaa cctgtacggt atggttaccg
    12841 gtatggtgga agatatgcag agtctggtcg gcggaacggt ggtcaggcgt aaggtttacg
    12901 cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat ccggagcagg
    12961 aggtgatcag tcgctggcgc atcgagcagt gcagcgaact gagcgcggtc agtgcctcct
    13021 ttgtactgtc cacgccgacg gaaacggacg gcgctgtttt tccaggacgt atcatgctgg
    13081 ccaacacctg cacctggacc tatcgcggtg atgagtgcgg ttatcacggt ccggcagtcg
    13141 cggatgaata tgaccagccg acgtccgata tcacgaagga taaatgcagc aaatgcctga
    13201 gcggctgtaa atttcgcaat aacgtcggca actttggcgg cttcctttcc attaacaaac
    13261 tttcgcagta atcccatgac agagacagaa tcagcgattc tggcgcacgc ccggcgatgt
    13321 gcgccagcgg agtcgtgcgg cttcgtggtg agagcgccgg agggggaaag atattttccc
    13381 tgcgtgaata tttccggtga gccggaggat tatttccgga tggctccgga ggactggctg
    13441 caggcagaga tgcagggtga gattgtggca ctggtccaca gtcatcccgg tggtctgccc
    13501 tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg gtggctggtc
    13561 tgccggggtg agattcacaa gttccgctgt gtgccgcatc tcaccgggcg tcgctttgag
    13621 cacggggtga cggactgtta cacgctgttt cgggatgctt atcatctggc ggggattgag
    13681 atgccggatt ttcatcgcgg ggatgactgg tggcgtaacg gtcagaatct ctatctggat
    13741 aatctggagg ccacagggct gtatcaggtg ccgttgtcag cggcgcagcc gggcgatgtg
    13801 ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg tggtgacggc
    13861 gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac cgacaaatgg
    13921 cagcgacgca cacactccct ctggcgtcac cgggcatggc acgcatctgc ctttacgggg
    13981 atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggcc gaagccatcc
    14041 gggcgctggc cacacagatc ccggcgtttc gtcagaaact gagcgacggc tggtatcagg
    14101 tacgcattgc cgggcgtgat gctggtgaaa ccgaattgtc tgcccgtctt aatgagccgc
    14161 tggcaaatgg tgccgtgatc cacatcgtgc cgcgtctggt gggagctaaa agtggcggtg
    14221 tgtttcaggt ggtgcttggg gcggcgctga ttgcggtggc atggtggaac cctgtgggct
    14281 ggctgggtgc cgcggctgta tcgggcatgt atgcggcagg ggccagtatg gtcctgggcg
    14341 gagtggcgca gatgctggca ccgaaagcca gaactcccac agcgaccagc acggataacg
    14401 gcaaacagaa cacctatttt tcttcgctag ataatatggt tgcacagggt aattcgctgc
    14461 cggtgctgta tggcgaaatg cgcgtggggt cccgggtggt atctcaggag atcagcacgg
    14521 cagatgaagg tgatggtggt caggttgtgg tgattggtcg ctgatgaaaa acgttttatg
    14581 tgaaaccgcc tccgggcggt tttgtcgttt atggagcgtg aggaatgggt aaaggcagca
    14641 gtaaggggca taccccgcgc gaagcgaagg acaacctgaa gtccacgcag ttgctgagtg
    14701 tgatcgatgc catcagtgaa gggccggttg aaggtccggt ggatggatta aaaagcgtgc
    14761 tgctgaacag tacgccggtg ctggacaatg aggggaatac caacatctcc ggcgtcacgg
    14821 tggtgttccg ggcaggtgag caggagcagt caccgccgga ggggtttgaa tcctccggct
    14881 ccgagacggt gctgggtacg gaagtgaaat atgatacgcc gatcacccgg accatcacgt
    14941 cggcaaccat cgaccgtctg cgctttacct tcggtgtgca ggcactggtg gaaaccacct
    15001 caaaggggga caggaatcca tcggaagtcc gcctgctggt tcagatacag cgtaacggtg
    15061 gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacctcgcag tatctggcct
    15121 cggtggtggt ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc
    15181 cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcatac accgaaatca
    15241 tcgatgtgaa acagtgctac ccgaacacgg cactggtcgg cgtgcaggtg gactcggagc
    15301 agttcggcag ccagcaggtg agccgtaatt atcatctgcg cgggcgtatt ctgcaggtgc
    15361 cgtcgaatta taacccgcag acgcggcaat acagcggtat ctgggacgga acgtttaaac
    15421 cggcatacag caacaacatg gcctggtgtc tgtgggatat gctgacccat ccgcgctacg
    15481 gcatggggaa acgtcttggt gcggcggatg tggataaatg ggcgctgtat gtcatcggcc
    15541 agtactgcga ccagtcagtg ccggacggct ttggcggcac ggagccgcgc atcacctgta
    15601 atgcgtacct gaccacacag cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga
    15661 tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac cgaccatcag
    15721 ataaggtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc
    15781 gctacagctt cagcgccctg aaggaccgcc ataatgccgt tgaggtgaac tggattgacc
    15841 cgaacaacgg ctgggagacg gcgacagagc ttgttgaaga tacgcaggcc attgcccgtt
    15901 acggtcgtaa tgttacgaag atggatgcct ttggctgtac cagccggggg caggcacacc
    15961 gcgccgggct gtggctgatt aaaacggagc tgctggagac gcagaccgtg gatttcagcg
    16021 tgggtgccga agggcttcgc catgtgccgg gtgatgttat tgaaatctgc gatgatgact
    16081 atgcggggat cagcatcggc gggcgcgtgc tggctgtgaa cagccagacc cggacgctga
    16141 cgctcgaccg tgaaatcacg ctgccatcct ccggtaccac gctgataagc ctggttgacg
    16201 gaagtggcaa tccggtcagc gtggaggtcc agtccgtcac cgacggcgtg aaggtaaaag
    16261 tgagccgtgt tcctgacggc gttgccggat acagcgtatg ggggctgaag ctgccgacgc
    16321 tgcgccagcg cctgttccgc tgcgtgagta tccgtgagaa cgatgacggc acgtatgcca
    16381 tcaccgccgt gcagcatgta ccggaaaaag aggccatcgt ggataacggg gcgcactttg
    16441 acggcgacca gagcggcacg gtgaatggtg tcacgccgcc agcagtgcag cacctgactg
    16501 ccgaagtcac cgcagacagc ggggaatatc aggtgctggc gcgctgggac acgccgaagg
    16561 tggtgaaggg ggtgagcttt atgcttcgcc tgaccgtggc agcggatgac ggcagtgagc
    16621 ggctggtcag cacggcccgg acggcggaaa ccacataccg cttcacgcaa ctggcgctgg
    16681 ggaactacag gctgacagtc cgggcagtaa atgcctggag gcagcaggga gacccggcgt
    16741 cggtatcgtt ccggattgcc gccccggcag cgccgtcgcg gattgagctg acaccgggct
    16801 attttcagat aaccgccacg ccgcatcttg cggtttatga cccgacggta cagtttgagt
    16861 tctggttctc ggaaaaacgg attgctgata tcaggcaggt tgaaaccaca gcccgctatc
    16921 ttggcacggc gctgtactgg atagctgcca gtatcaatat caaaccgggc catgattatt
    16981 acttttatat ccgcagtgtg aacaccgttg gcaaatcggc attcgtggag gccgtcggtc
    17041 gggcgagcga tgatgcggaa ggttacctgg attttttcaa aggccagata accgaatccc
    17101 atctcggtaa agagctgctg gaaaaagtcg atctgacgga ggataacgcc agcagactgg
    17161 atgagttttc gaaagagtgg aaggatgcca acgataaatg gaatgccatg tgggctgtca
    17221 aaattgagca gaccaaagac ggcaaacatt atgtcgcggg tattggcctc agtatggagg
    17281 acacggagga aggcaaactg agccagtttc tggttgctgc taaccgtatc gcgtttattg
    17341 acccggcaaa cgggaatgaa acgccgatgt ttgtggcgca gggcaaccag atattcatga
    17401 acgacgtgtt cctgaaacgc ctgacggcac ccaccattac cagcggtggc agtcctccgg
    17461 tattttccct gacatcagac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg
    17521 tgaatgcgaa ctcagggacg ctcaacaacg tcacgattaa tgagaactgt cagattaagg
    17581 ggaaactgtc agccaaccag attgaaggtg atattgtcaa aacggtcagc aagtctttcc
    17641 cccgcacgag cacttatgcc agtggcacca tcacggtaag aatcagtgat gatcagaaat
    17701 ttgaccggca ggtcatgata ccaccagtgt tattccgcgg tggtaagcat gagaatttca
    17761 acagtaataa ccaacagtca tactggtatt caacctgccg gttaagagtg acccgcaatg
    17821 gtcaggagat ttttaatcag tccacgacgg atgctcaggg cgtattttcc tcagttatag
    17881 atatgcctgc cggacagggg acgctgacac tgacattcac cgtatcttca tcaggagcga
    17941 ataactggac accaacaacc agtatcagcg atctgctggt tgtggtgatg aaaaaatcca
    18001 cagcaggtat cagtatcagc tgaattttat aacccataac gggcgtcaga aatgacgcct
    18061 tttttattgc agaaaagcga gaggtaatta tgcgtaaact ttatgccacc attttgtccg
    18121 cagccatctg tctggccgta tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc
    18181 tgagcgcggg ctatcttcat gcctcgacga acgtttccgg cagcgatgat ctgaacggga
    18241 ttaacgtgaa ataccgttat gagtttacgg acacactggg gctggtgacg tcattcagct
    18301 atgcaggaga caagaatcgc cagcttaccc gttacagcga tacccgctgg catgaagatt
    18361 cagtgcgtaa ccgctggttc agcgtgatgg cggggccgtc tgtacgcgtg aatgaatggt
    18421 tcagcgcgta tgcgatggcg ggtgtggctt acagccgtgt gtcgactttc tccggggatt
    18481 atctccgcgt aactgacaac aaggggaaaa cgcacgatgt gctgaccgga agtgatgacg
    18541 gtcgccacag caacacgtct ctggcgtggg gggctggcgt gcagtttaac ccgaccgaat
    18601 ccgtggccat tgatattgct tatgaaggct ccggcagtgg tgactggcgc actgacggtt
    18661 tcatcgtggg tgtcggttat aaattctgat tagccaggta acacagtgtt atgacagccc
    18721 gccggttcag gcgggctttt ttgtggggtg aatatggcag taaagatttc aggtgtactg
    18781 aaagacggca caggaaaacc ggtacagaac tgcacaatcc agctgaaagc aaaacgtaac
    18841 agtaccactg tggtggtgaa cacgctggcc tcagaaaatc cggatgaagc cgggcgttac
    18901 agtatggacg ttgagtacgg tcagtacagc gttattctgt tggtggaggg attcccgccg
    18961 tcacatgccg ggaccatcac cgtgtatgaa gattctcaac ccggtacgct gaatgatttt
    19021 ctcggtgcca tgacggagga tgatacccgt ccggaggcac tgcgacgttt tgaactgatg
    19081 gtggaagagg tggcgcgtaa cgcgtccgcg gtggcacaga acacagcagc cgcgaagaag
    19141 tcagccagca atgccagcac atcagcccgt gaggcggcaa cccatgcgac tgatgctgca
    19201 ggctcagcac gcgcagccag cacgtcagcc ggacaggccg cgtcgtcggc tcagtcagcg
    19261 tcttccagcg caggaacggc atcagcaaag gccactgaag catcaaaaag tgctgccgct
    19321 gcagagtcct caaaaagcgc ggcggctacc agtgccggtg cggcgaaaac gtcagaaacg
    19381 aatgcggcag cgtcacaaca atcagcagcc acttctgcat ccaccgcgac cacgaaagcg
    19441 tcagaagcag ccacttcagc acgggatgcg tcggcttcaa aagaggcggc aaaatcatca
    19501 gaaacgaacg cagcctcgag cgccagcagc gcagcttcct cggcaacggc ggcaggaaat
    19561 tccgcgaagg cggcaaaaac gtccgagacg aacgccaggt cttctgagac ggcagcggga
    19621 cagagcgcct cagctgcggc aggctcaaaa acagcggctg cgtcgtctgc cagtgccgcg
    19681 tcaacaagtg ccgggcaggc ctcagtcagt gccaccgccg ccggaaaatc ggcagaaagc
    19741 gccgcatcat ccgcttcaac agccacaacg aaggctggcg aagccactga gcaggccacg
    19801 gcagcagcga ggtcagcttc cgcagcgaag acatccgaaa cgaacgcgaa agcgtcggaa
    19861 acccgtgcag aatcctcaaa aacggcagcg gcatcgtccg ccagttcggc ggcgtcatcg
    19921 gcctcatcgg cgtctgcttc aaaagatgag gcgaccagac aggcgtcagc agcgaagggc
    19981 agcgccacga cggcatccac gaaggcgaca gaggctgctg gtagtgcgac ggcagcagct
    20041 cagagcaaaa gtacggcgga atctgcagca acgcgcgctg agacagcggc aaaacgggca
    20101 gaggatattg catccgccgt ggcgcttgag gatgcgagca cgacgaaaaa ggggatagta
    20161 cagctcagca gtgcgaccaa cagtacgtct gaaacgctgg cggcaacgcc aaaggcagta
    20221 aaatcagcct atgacaatgc agagaaacgt ctgcagaaag accagaacgg cgctgatata
    20281 cccgataagg gacgcttcct gaacaacatt aacgcggtca gtaaaacaga ctttgctgat
    20341 aagcgtggta tgcgttatgt gcgggttaac gctcctgcag gtgcaacatc tggaaaatat
    20401 taccctgttg ttgttatgcg ttctgctggc tcagtaagcg aactggcatc aagggtcatt
    20461 atcaccacgg caacgcgaac cgcaggcgat ccgatgaata actgcgagtt taacggattt
    20521 gttatgcctg gtggctggac tgacaggggg cgttatgctt atggaatgtt ctggcaatat
    20581 caaaacaatg aacgagccat ccactcaata atgatgagta ataagggcga tgatttgcgc
    20641 tctgtgttct atgttgatgg cgctgctttc cctgtttttg cgtttatcga agatggcctg
    20701 tcaatatccg cacctggtgc tgatctcgtt gttaatgata cgacctataa gtttggggca
    20761 acaaatccag cgactgaatg tatcgcggcg gacgttatcc ttgattttaa gagtgggcgt
    20821 ggtttttatg agtctcattc gttaatcgtt aacgataact tgtcgtgcaa aaaacttttt
    20881 gccacagacg aaattgtagc gcgtggtggt aatcagattc gaatgatagg tggggagtat
    20941 ggtgcattat ggcgtaatga tggcgctaaa acttacctgc tgcttaccaa tcaaggtgat
    21001 gtttatggtg gctggaatac attaagaccg tttgctattg ataacgcaac cggcgagctg
    21061 gttattggaa ccaaactgtc cgcaagtctg aacggtaatg cattaacagc aacaaagctg
    21121 caaacgccaa gacgggtttc tggtgttgag tttgatggtt ccaaagatat tactttaacc
    21181 gccgcgcatg tggctgcttt tgccagaaga gcaacggata cgtatgccga tgcggatggg
    21241 ggcgttccat ggaatgccga atcaggcgct tacaatgtca cccgctctgg cgacagctat
    21301 attctggtta acttctatac cggagtcgga agttgccgga ccctgcagat gaaggcgcat
    21361 tacagaaatg gaggtctgtt ctaccgttcc tcaagagatg gctatggttt tgaggaagac
    21421 tgggcagaag tttatacctc gaaaaatctt ccaccagaaa gctacccagt cggcgcacca
    21481 atcccgtggc catcagatac cgttccgtct ggttatgccc tgatgcaggg gcagactttt
    21541 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
    21601 cgtagctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
    21661 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
    21721 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcatacgcac
    21781 agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca
    21841 gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg
    21901 aatacaagtg ctgcaggagc gcacactcat agtgttagcg gcacagccgc atctgcaggt
    21961 gctcacgcac atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat
    22021 ggacacacta tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca
    22081 tttaactaca ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
    22141 taaaaattta taatctgctg gccggaacta atgaatttat tggtgaaggt gacgcatata
    22201 ttccgcctca tacaggtctg ccagcaaaca gtaccgatat tgcaccgcca gatattccgg
    22261 caggcttcgt ggctgttttc aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc
    22321 ggggtaaaac ggtttatgac gtagcgtcag gggacgcgtt atttatttct gaactcggtc
    22381 cgttaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
    22441 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
    22501 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccgcttcag gatgctgcag
    22561 atctggaaat tgcaacggag gaagaaatct cgttgctgga agcatggaaa aagtatcggg
    22621 tattgctgaa ccgtgttgat acgtcaactg cacaggatat tgaatggcca gcactgccgt
    22681 agggtaaaac atataaattc tataattaga tgtatctttc catttacggc aaggaagggg
    22741 gcttggaaga cgtaaagcat ctcacaccaa gattattttt tatatgtcag gtgtctgaag
    22801 ttttgctttg gctcttaaaa tggtttgccg cgaggttttg aattcccggg caatggcact
    22861 tatacttaca cctgacttaa tccgttcgaa taccacctgt ttctgttctt catttaacac
    22921 aggtggtcga ccaaaacgtt tccctgcgcc ccgggctctt actatcccgg aatgagtgcg
    22981 ttcaagtaaa aggtctcgtt caaattcagc gactgctgaa attacttgca tcatcatttt
    23041 tcctgttgga ctggtcaggt caatgccccc caatgctaag caatgcactc tgatacctgt
    23101 ttcggtcagt tgttccactg ttttcctgat atccattgca ttacaaccaa ggcgatccag
    23161 ttttgtcaca atcaattgat caccacattt caggcgagca agcaaccggt taaaaccagg
    23221 acgctcactg gttgctgctg agccgctaat gtgttcttcg attatttgct gaggtttgat
    23281 tttaaaacct gcactttcga tttcccggcg ttgattttcg gtggtctgat ccagcgttga
    23341 tatccgacag taagcaaaaa ttcgagacat agtgagactc tatacgaaat tggtgtacat
    23401 atcataatgc atatccaaaa attatttcgc ttattattgt acatatttgt atgtatacgt
    23461 tcgaaaataa acgaattcgg atgcagtccc gtaattttgg tgtaaccaaa aatcgatttt
    23521 ttgaaaaatg gctttaactc ggtttgtttt tcgaactc
//
LOCUS       DAZUGK010000056        23468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZUGK010000056 DAZUGK010000000
VERSION     DAZUGK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.056"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..676)
                     /gene="focB"
                     /locus_tag="ACHY5W_001949"
     CDS             complement(<1..676)
                     /gene="focB"
                     /locus_tag="ACHY5W_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFS4446853.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHF"
     gene            complement(698..2710)
                     /gene="hyfR"
                     /locus_tag="ACHY5W_001950"
     CDS             complement(698..2710)
                     /gene="hyfR"
                     /locus_tag="ACHY5W_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFS4446854.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(2740..3153)
                     /gene="hyfJ"
                     /locus_tag="ACHY5W_001951"
     CDS             complement(2740..3153)
                     /gene="hyfJ"
                     /locus_tag="ACHY5W_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFS4446855.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDGMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYKDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3146..3904)
                     /gene="hyfI"
                     /locus_tag="ACHY5W_001952"
     CDS             complement(3146..3904)
                     /gene="hyfI"
                     /locus_tag="ACHY5W_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFS4446856.1"
                     /translation="MSPVLTQHVNQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3901..4446)
                     /gene="hyfH"
                     /locus_tag="ACHY5W_001953"
     CDS             complement(3901..4446)
                     /gene="hyfH"
                     /locus_tag="ACHY5W_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFS4446857.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(4456..6171)
                     /gene="hyfG"
                     /locus_tag="ACHY5W_001954"
     CDS             complement(4456..6171)
                     /gene="hyfG"
                     /locus_tag="ACHY5W_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFS4446858.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFAFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHVFALGFVEAPRGEDVHWSMLGDNQKLLRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6161..7742)
                     /gene="hyfF"
                     /locus_tag="ACHY5W_001955"
                     /pseudo
     CDS             complement(6161..7742)
                     /gene="hyfF"
                     /locus_tag="ACHY5W_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(7747..8397)
                     /gene="hyfE"
                     /locus_tag="ACHY5W_001956"
     CDS             complement(7747..8397)
                     /gene="hyfE"
                     /locus_tag="ACHY5W_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFS4446859.1"
                     /translation="MTGSTIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8409..9848)
                     /gene="hyfD"
                     /locus_tag="ACHY5W_001957"
     CDS             complement(8409..9848)
                     /gene="hyfD"
                     /locus_tag="ACHY5W_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFS4446860.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQAEKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(9865..10812)
                     /gene="hyfC"
                     /locus_tag="ACHY5W_001958"
     CDS             complement(9865..10812)
                     /gene="hyfC"
                     /locus_tag="ACHY5W_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFS4446861.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLSMALPLFIAVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVMMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10823..12841)
                     /gene="hyfB"
                     /locus_tag="ACHY5W_001959"
     CDS             complement(10823..12841)
                     /gene="hyfB"
                     /locus_tag="ACHY5W_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFS4446862.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHGGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGTISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTD
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12841..13458)
                     /gene="hyfA"
                     /locus_tag="ACHY5W_001960"
     CDS             complement(12841..13458)
                     /gene="hyfA"
                     /locus_tag="ACHY5W_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFS4446863.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13711..14181)
                     /gene="bcp"
                     /locus_tag="ACHY5W_001961"
     CDS             complement(13711..14181)
                     /gene="bcp"
                     /locus_tag="ACHY5W_001961"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFS4446864.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHLVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14181..14753)
                     /locus_tag="ACHY5W_001962"
     CDS             complement(14181..14753)
                     /locus_tag="ACHY5W_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFS4446865.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14899..15777
                     /gene="dapA"
                     /locus_tag="ACHY5W_001963"
     CDS             14899..15777
                     /gene="dapA"
                     /locus_tag="ACHY5W_001963"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4446866.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15794..16828
                     /gene="bamC"
                     /locus_tag="ACHY5W_001964"
     CDS             15794..16828
                     /gene="bamC"
                     /locus_tag="ACHY5W_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFS4446867.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17041..17754
                     /gene="purC"
                     /locus_tag="ACHY5W_001965"
     CDS             17041..17754
                     /gene="purC"
                     /locus_tag="ACHY5W_001965"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4446868.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17922..18785
                     /locus_tag="ACHY5W_001966"
     CDS             17922..18785
                     /locus_tag="ACHY5W_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFS4446869.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18800..20815
                     /gene="tmcA"
                     /locus_tag="ACHY5W_001967"
     CDS             18800..20815
                     /gene="tmcA"
                     /locus_tag="ACHY5W_001967"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFS4446870.1"
                     /translation="MAELNALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRIEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQQNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            20889..21587
                     /gene="ypfH"
                     /locus_tag="ACHY5W_001968"
     CDS             20889..21587
                     /gene="ypfH"
                     /locus_tag="ACHY5W_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4446871.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21698..21898)
                     /locus_tag="ACHY5W_001969"
     CDS             complement(21698..21898)
                     /locus_tag="ACHY5W_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFS4446872.1"
                     /translation="MDWLAEYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDEDDWPKKDQPKK"
     gene            complement(21926..23053)
                     /gene="dapE"
                     /locus_tag="ACHY5W_001970"
     CDS             complement(21926..23053)
                     /gene="dapE"
                     /locus_tag="ACHY5W_001970"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFS4446873.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23057..23413)
                     /locus_tag="ACHY5W_001971"
     CDS             complement(23057..23413)
                     /locus_tag="ACHY5W_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFS4446874.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5848 a         6219 c         6305 g         5096 t
ORIGIN      
        1 caaaatggcg aatggcaatg gcgaacggaa tcacaaacaa attggcgata cagtgctcaa
       61 agccactggc gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataatg
      121 aacgcccgca ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga
      181 ggctgacaga ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga
      241 ctccccacat cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac
      301 aggcaatacc tgccagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc
      361 aactaataac gccccgactt tttgccatca ccgtcattac cgacgaggtg aacaggctgg
      421 tgccgcaaac cgccagcaaa ataaagccga gtgtaaagca aaggccgccc accagatggg
      481 ttaatgcctg tgaagacggg gcatcggcga taacggaaag gtaaaaagta aatccaatcg
      541 ccataaacac gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt
      601 tatgggcggc aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag
      661 agagtttgtt tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga
      721 tccccagacg ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg
      781 cgccacgggg cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac
      841 gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa
      901 gcgctgaatc ttcatttagc gtcggcagta aacggctttg tctgacatta agatgtaaat
      961 tcagactgtt accgcgagtc agcagtaccg cccgctcaat cacgttttcc agctcgcgca
     1021 cgttgcccgg ccaatcccac gacatcaact ggcgtagtgc ctcagtcggg atggcgtcaa
     1081 ttgcgcgatt catatggcgc gccatttttt gcgtgaaatg ttttgctaaa agagggatat
     1141 cttccggacg gtcgcgtagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga
     1201 gatcgctgcg aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa
     1261 tgactctgac attcaccggg atcgttctac tcccgccgag acgctcaatc tcccgctcct
     1321 gcaatacgcg cagcagttta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa
     1381 acaacgtgcc gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa
     1441 acgccccttt gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc
     1501 agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga
     1561 tcacctcttt gccagttccg gtttcaccgc aaatcaacac cgtactgtcg ctcttcgcca
     1621 caatatctac ctgctggagc aggtcttcca ttgcctggct ttgatagata atgtcaccga
     1681 cgcccagatt cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct
     1741 gcaaatcggt catgctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg
     1801 ctatgtgttg tagtagctgg cagttttcct cactaaagag agtggaagag gtatgcgcca
     1861 gcaacaatgc acccggtgta tggttgccaa aggtaagcgg tatcaggagc gcagattcgc
     1921 agccgttaga tgcaagtaag tgtaataacg ggtcgcgttg ccagagaaac agatcgtctg
     1981 cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc
     2041 caggcatatt gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc
     2101 tgaacttctc gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa
     2161 aatgatggat ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca
     2221 ctgcattagt gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca
     2281 gcgccacctg ttgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg
     2341 ccagccagtt aaacagtgtg caatcctggg gagtaaaacg gctgccgtgg gtacgaataa
     2401 agcgtagctc cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgacca
     2461 tctcgccctg atactctact gttgccgtca catcgctgcc atcatcatgg tagcggtaat
     2521 agaagttgct tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca
     2581 gcgggatgaa ggcgcgtaat aaagacgcct tgccgtgttt ctgtgctaac cgctccgcga
     2641 gcattccgtt taccgcttca attgttattc cttgtggcgg ggcaaacatc gcctcgtctg
     2701 acatagccat caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat
     2761 cagatagatg gccggttcct gcacaatttc atccaacaga tgaatgaatt gaatgctacg
     2821 cgcttgccac acagacgtcg cgtcatctgc cagtggcgcg aatgcccggg tcaacagggc
     2881 ggtgtggctg gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg
     2941 ggcttgctcc tcttcgacca gtgcaagcca atctttgtat tccgtcagtg ggcagcggaa
     3001 ggcgacattc agacaatcaa tcacgccaac gtgatggccg atagctagcg agtaatacat
     3061 tacctgagag ctgtgttccg gcatcccgtc gctactggcg acaaactttt ttcgcagcgt
     3121 ccagaaaaca atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt
     3181 gctgaacgat actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat
     3241 ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc gcaaatttct cgcccctgac
     3301 gatagcccgc cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct
     3361 gcggccacaa cggttgcata gtcaccccgg tgggatcgcg ataatccacc gcgtgaatct
     3421 tctgttgcag caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg
     3481 ggcagccggg gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt
     3541 agagatcgtg gaaaataccg ccgccgacac cgcacgcgcc gtaggaaaca cagattttat
     3601 gatcgggggc agactcatac gcccgaagtg caggcatacg catcgcccgg gtgactgcgc
     3661 cagtaaataa caaaatatcg gcgtgacgcg gtgatgaaac aaccttaatg ccaaaacgtt
     3721 ctgcgtcgaa tactggtgtg atggcggcaa aaatttcgat ttcacaggcg ttgcagccgc
     3781 cgcaatcgac gcgataaacg taggctgagc gacggatatc ctgtagcaaa tgccgcttca
     3841 tcttttgcgt ttgctcgtct agcgtgatgg gctggttgac atgttgtgta agcactggac
     3901 tcatagctgc tccttagcca ccagcggtac atctgtatcg tcgttgatca gcgtcgcgcg
     3961 ttgtttacac tccgggcaga cgctcgcttg cgcccgcaac atttcgcggt tttgtggcgc
     4021 attttgttgc tgtgccaaca attcagcagc cagtgcgacg gttttttgcg gggcaaacgg
     4081 gcgttcgcaa cggctgcaac gttgtagatg gaacgtcgcg cgggtataga gatcggcttt
     4141 attggtgacg gtcagttcaa agttattggt aagctggatg gctctggtcg ggcacacttc
     4201 ttcacaacgt ccgcagtaaa tacaacgccc cagatagagc tgccaggtgc gcgaattttg
     4261 ctggtcgtcg gtctggatag tcagcgcatt tgccggacaa gcacaggcgc aggcaccgca
     4321 ggcaatacat tggctgggca tcaggtccgg tttcccgcga aagcctgggc tgacctccag
     4381 tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt
     4441 cagcatcttc tgtccttact tcagcggcga acggttacga tcaatgccgt agcgttcgat
     4501 ctctttatac ggcacggttt ttgactggcg cttgcgcaca tcaaccagcg tcacacggtc
     4561 ggtacaggag tagcagggat caaggctacc gataatcagc ggtgcgtcag aaacggtatt
     4621 gccgcgtagc atgtaacgca gcaccggcca gttggcgtag gtggcggcac ggcagcgcca
     4681 gcggagcaat ttttggttat caccgagcat gctccagtgc acgtcttcac cgcgtggcgc
     4741 ttcgacaaag cccagcgcga atacgtgagg tttatagcta aagccttcgg tcagcagtgg
     4801 agtatccggc atgttgtcga gggcaaattc cagcattgcc agcgaatcaa acgtctcttt
     4861 gacacggacc atcacgcggg agaagacatc gccgccggta aaggcaaaca gtgtttttgg
     4921 aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcaa aaccactgcc
     4981 gcggatcagc ggtcctacgg ggctataatc acgggcgatt tgtcggtcga gaatgccaat
     5041 gccctgagtg cgttgttcca tattcggcgt ggcaagcagc atttctacca gctccgacac
     5101 gtcggcgcgc atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc
     5161 gcggcgaaca ccaccaatca gattcagacc gtaggtttta cgcgacccgg tcagcaattc
     5221 cgccattgtc atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaatcc
     5281 aacaaaatgg caggagaggc caaggttgag caaatgactg tgtagccgtt cgacttccag
     5341 cagaatcgag cgaatggtat gcgctcgttg cggcacctca atccccagtg cattttcaac
     5401 cgagttggta taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa
     5461 gaaggtcact tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac
     5521 ataaaacagg cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg
     5581 acccggttca tcggaggtga tatgcagcgg gccgacaggg atcacccgcg catcgctgtt
     5641 gccctcattg ataaacggat acgtttcgga atcagtcgtc ggttcagggc gcagtcgata
     5701 atccatcgca tctttgcgta gcggatgcat atcttccggc cagtcatcgg gcaacaccag
     5761 gcgacgctga tccggcaggc caaccggaat cagcccgtac atatcgcgaa tttctcgctc
     5821 gccccagacc gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc
     5881 gaccagtgcc ttcaccacga tccagcattt ttcggcccct tccattgaaa gggcataata
     5941 aaccgcgtaa tgaccgttaa gtgtccgctc gtcgttgcca aacaggaccg gaagccagcc
     6001 atcatgttga taataaagat actgtacaac gtcaggcagc agattgattt tcaccgtaat
     6061 ggtgacctgt tcaggcgttt gtcgctcttc atccagcacc gcgccgggga actgcgtttt
     6121 cagggcggcg agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct
     6181 ccgaaacaga tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca
     6241 ggtcgtgcgt ccctgagagg actatagtgg aagcgcccgc caggatcctg atgacaggtt
     6301 gtggaatatg cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg
     6361 tcaaccagcc gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg
     6421 cagccatccg taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa
     6481 tcagcaggtg attacgtgcc agtccggcgg taacggtcat aaattcgcta agaaaaatgt
     6541 tgaagggcgg catccctgcc agcgccagcg caccgccgcc aaacagcacg gcggtaaatg
     6601 gcatgatttt gagcatccca cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta
     6661 cattgccgga accgcagaac agcagcgttt ttgccagact gtggtttaag gtgtgcagca
     6721 gcgcggcaaa aattcccagc ggcccgccaa tgcctagcgc caccgcgacc agtcccatgt
     6781 tctccacgct ggagtacgcc agcagacgct taatgtcccg ctgtaccaga atgaaaaatg
     6841 ccgccacggc aaccgacaac atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc
     6901 cgatggcctg gcaaataatg atgtaatagc gaatcagcac caacagcgcg cagttcagca
     6961 atacggcgga gagtagggcg ctgaccggac tcggcgcttc actgtgagca tccggcagcc
     7021 aggcgtgcat gggaaatagc ccggttttgg taccaaagcc aattagcaca aacacaaagg
     7081 ccaacagcat taatgttggg tcaagcaagg acgattgctt aagaacctcg ctccagaata
     7141 tcgccatttc tgcctgcggc ataacgctgg cggcgttggc gtataccagc acggtaccga
     7201 acagaccaaa agcgacacca acagtacaaa taatgatgta cttccatgca gcttccagcg
     7261 atgaacgctg accgtaaatg cctaccagaa acgccgagct taaggtggtg gcttcgatcg
     7321 ccgcccacat cacaatcagg ttattgctgg taacaaccag cagcatggtg aacaaaaaca
     7381 gatggaagaa accgtagtaa tcgcacagcg taacgggtga aagctcgccg tgtgccactt
     7441 catgacgcat gtagccaatc gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg
     7501 cgaggaacaa accgccccag accatcaata tgaagccaca gtcccgcagc gaatatttct
     7561 cctgcatcag cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct
     7621 aagctatgta atacggtcac cgtgcgagtc gcagagagcc tccgtttccg gcaggcaaaa
     7681 cagagcagcg aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc
     7741 atctcattat cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca
     7801 tatttttctt gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc
     7861 aatttccacc agttccggtg cacgccaggc tagaagcgcc agcaccagat gggagccgtt
     7921 ttccatcagg cagtaaccaa aaatttgccg caggatattg cgctggctaa caatgcacag
     7981 caggccaagc agaaaatgac ctaacgccac cgccagcgct ggtttcagcc cggtagccat
     8041 cggtagcttc acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag
     8101 tgccatcatt gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc
     8161 agcgtaagtc atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca
     8221 gatcagcagt tgctctgcgg cgaacaggca cgagagggtg gcgaaaatag acaccagcac
     8281 cagtgactgg caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa
     8341 cagcgatgtc agcatcatca gtcccgccag attatttacg atcgtagaac cggtcattca
     8401 tcatctcctt actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc
     8461 agtacaatca gcaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg
     8521 ctcggtttgc cggggacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc
     8581 gattcaatca tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca
     8641 aaaccggcag caaacagcgg gaatttactg aagaagccgt tgaacggcgg tacgccggta
     8701 atcgccagcg ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca
     8761 cgcagacgcg gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa
     8821 aacaggcttt tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagcgctagc
     8881 cgcgagccga agatggagag cgacaaaccg aagaacatcc agccaagttg agtgatggtc
     8941 gaccaggcca gcaaccgctt catatcctgc tgtggcaaat acatcagaaa tccataaaga
     9001 atggtgacca gtgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg
     9061 tcgataatgg cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga
     9121 taggcgctga tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc
     9181 tgggccgatt tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg
     9241 ccgtgtaact cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg
     9301 gcggcaagat acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa
     9361 cgctgcgctt tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt
     9421 tcaaaaaaca acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg
     9481 ataaacacca gcaaaaatgc ataataacga ttcgtgccgt tatgcgggtg ttcgcgattt
     9541 ttatccgtca gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca
     9601 aacagaatca gcgtactcac gcgatcaatg accagaccaa acaatgccac atcccccacg
     9661 ttgaccaacg tcaacgtgac ggcaactttc tcggcctgat aaaacgcgga gatcagtgac
     9721 aacatgcaca gcgtggtcag cgcggcgaac aaaacccccc attcggcggc ccgacgttgt
     9781 ggcgaaaacg aaacgaccag tgcgccgata aaaggcagca ataacgtcgt cagagcaaga
     9841 ttttccatat tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc
     9901 agcaaggctg aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag
     9961 cgtgttttct gcgatagagg ccagaacaaa aatcagcaaa accttgagca gcgtaacgac
    10021 aagtgaagtc agcaggcagg cgagagaaag ctcttgcgcg cgcccaaagg gcagaaacag
    10081 ggccacaaac agtgctgcca tcatgacctg tttcagcccc agcccccatt tcgccagcgc
    10141 cagcccggca ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc
    10201 aacatcaaag ggaatttttc ccatctctat gaagcaggca aaaccacagg ccagtaacgc
    10261 cagtacggtg gttagcggcg agttccagcc catcgccagc gtattgctga tcatctcgat
    10321 atgcgtggaa tccgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac
    10381 cagaatgccg agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc
    10441 cagccctgaa agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag
    10501 atcgccgccg cccgcaaaag gggaaacggc aataaatagt ggtagggcca tcgacagcac
    10561 cagcatgctg ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg
    10621 cgcaacttcc tgccgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg
    10681 tccgcggcgg gagtgcattc gcgcgcgtat ctgccgggaa atacccgtaa aaagcggggt
    10741 tagcagcagc agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag
    10801 agtttgtctc atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg
    10861 cgaccacata caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg
    10921 ccagccgttg ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg
    10981 tgctctgagc cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga
    11041 gctgttgtcg tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat
    11101 tgcctgacgg ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc
    11161 gacgaaacgc tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca
    11221 tcgccagtag taacagcaac agtaatgagg gagtgacccg tgtatgaaac gtgccgggta
    11281 caagtgctat tccgctggcg acagtgacgg gaggggtatc ggtaaacgca tgggcaatat
    11341 gcattatctt cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga
    11401 gcagtagcat ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac
    11461 tgcgcggcgc accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc
    11521 caccagtgac tgccagcatc accatagcaa taggacccgc aagttgtagc gctacggctt
    11581 ccacacgact tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca
    11641 gcggaggaat ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca
    11701 tccgtttcgc cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg
    11761 cgcccgcgcc gagaaatagt agccctttga acagcgcatg gttgagcaga tgaaacagtg
    11821 cgccgagcag tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca
    11881 caccgactgc cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt
    11941 tgatatcctg ttccgccagc gcatatagca cgcccaggag cgcggagatt gtgccgatcg
    12001 ccatcaccag aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca
    12061 ctttcaggat gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt
    12121 gcgatggtgc ggcagggtga gcgcgcggca accagctgtg caacggcatc attccggctt
    12181 tcgcgccaaa accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg
    12241 aaagcgtgcg gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg
    12301 cgatcataat cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga
    12361 tcgacgtttt atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa
    12421 acagcacgat gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga
    12481 aaatattcat aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag
    12541 tcaatgaata gagcgaacaa actaccacca gcaaggagat aaccagcacc ataaatgccg
    12601 agaggctatc cattcgcagg gttaaatcgg caaagtcaaa aggggtggca aaacgggcga
    12661 ctaaagttac gcctccatgt aattgcgtaa ttccgctgat aatgccaatc acgccgccca
    12721 ccagcgatgt gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg
    12781 aagccagact ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca
    12841 ttagcgttgc tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc
    12901 ggcaaggcgc tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa
    12961 cgcctgattc gggcaagcgc gaacgcaggc cggaccttgt ggcaagaaat cacacaggtc
    13021 gcatttcacc gcgacggtct ggacacccgg ttcccagcgc agcaaagcat gttgtgttgg
    13081 cgcgttttct tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc
    13141 attcaccgga cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag
    13201 cttgcagcca atacagaggc tttcgttgag ttggatcgca tcatccctct gggagatggc
    13261 attgaccggg cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc
    13321 agtgagtgtt gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt
    13381 atgcacgtcc gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc
    13441 caccacaaag cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt
    13501 gcataaagga tgccagtctg attaatttaa tatattcaat gagttatgaa ttaactggat
    13561 tctgaacggt acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt
    13621 gtcgcagggg aaagtgagaa tgtgatcttg atcacgagaa gtgaggtgtg ctggccgcaa
    13681 gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac
    13741 aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc
    13801 gtcaatcagg aagctgatgc gatgaatacc atcgtaggtt ttgcccatga aggacttttc
    13861 cccccagacg ccgaattgct cgcacaccag gtggtcctca tcagacagga gcgtaaagtt
    13921 aagcagctct ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag
    13981 cacatcaacg cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac
    14041 ggtacagccg ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa
    14101 gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc
    14161 accggctttc agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg
    14221 acgacgttaa tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca
    14281 atatttgctg cgtccgcaga tgcggggctg tgggcagtaa tctgaatatg caactgtgca
    14341 gcccgttcat tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc
    14401 gtgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga
    14461 acccagacag atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt
    14521 aaaagatcca gttcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc
    14581 catgaaccgg aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg
    14641 tcttcaatat tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg
    14701 cgatcggcac ccaacgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt
    14761 attcctttaa ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat
    14821 cagaacggtt ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt
    14881 ttgcacagag gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat
    14941 gaaaaaggta atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc
    15001 ggtacttcgg cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac
    15061 gaacatgctg atgtggtgat gatgacgctg gagctggctg acgggcgcat tccggtgatt
    15121 gccgggaccg gtgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac
    15181 agtggtatcg tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt
    15241 ttgtatcagc atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat
    15301 gtgccgtccc gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta
    15361 aaaaatatta tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa
    15421 gagctggttt cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc
    15481 atgcaattag gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg
    15541 gcccagatgt gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag
    15601 cgtctgatgc cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg
    15661 gcatgtaagg aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc
    15721 accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt
    15781 ttagggagat ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt
    15841 ttcgcttgtt ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag
    15901 tggtgatgaa gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat
    15961 gattttgccg gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt
    16021 cggtaaggcg ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg
    16081 tacccagttc acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct
    16141 gtggccgcag gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga
    16201 tgctggtcag acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca
    16261 gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt
    16321 taaactgttg aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg
    16381 ttacagcacg gagatgatga acgttatttc cgctggtctg gataaatctg ccactgacgc
    16441 cgcgaacgct gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga
    16501 caccggttta ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc
    16561 agcggcgctg gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat
    16621 ggccgtaact tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc
    16681 aggcctggca tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct
    16741 acagttcatc gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc
    16801 tgtcttccag gctgcgttta gcaagtaaaa gtacagggct ggaatcatcc ggcccttttt
    16861 tctgatatga tacgcaaacg tgtgcgtcgg taggaaaacg cgattttagc ggtaattcgc
    16921 acgaaatttg tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca
    16981 tccggcacac cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag
    17041 atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac
    17101 ccggacctgt tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt
    17161 gagcagtttg atcgcaaagg tatggtgaac aacaagttca actacttcat tatgagcaaa
    17221 ctggctgaag cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg
    17281 gtgaaaaagc tggatatggt accggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct
    17341 ctggtgaaac gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg
    17401 tttctgaaaa acgacgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt
    17461 ggctgggtaa gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac
    17521 gtgctgaaaa aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt
    17581 ggtctgtaca aaggtgaagt ggtactgggt gatgagttct ccccggacgg cagccgcctg
    17641 tgggacaaag aaacgctgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc
    17701 ctgatcgagg cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc
    17761 tgttcatcat cttgccgtgc tgctggcacg gcaagacaac cattctcgta agatgtgcat
    17821 tgaacgctca ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca
    17881 cctacgatca tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac
    17941 gtgaaagtga caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc
    18001 ccggtttccg cctgccgagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg
    18061 caggctacta tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tctcaacagc
    18121 aatcaacgcg gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc
    18181 tggcaaccac ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc
    18241 aaccgaagct ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca
    18301 taatggggcc gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg
    18361 atgacatgaa agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc
    18421 atgaagtcgg tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc
    18481 aacaaaacgc gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg
    18541 actgttttgc cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg
    18601 atctggaaga ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa
    18661 gtcaggggcg agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct
    18721 ggtttaaacg tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca
    18781 tttaacttcc ggggcaggga tggctgaact gaatgcgctt cacacattaa cagcgcaaat
    18841 gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga
    18901 tcatgcgctt aagttacgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc
    18961 agatgctgaa aaccactgtt ctccctcggc actacaaact ttacttggac gcgagttccg
    19021 gcatgcggta ttcgacgccc gccacggctt tgatgccgct gcctttgccg cacttagcgg
    19081 aacgttgaaa gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa
    19141 ccaacctgat gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca
    19201 ttttgtccag catttcaaac gcgtacttac ggcggataac gacgctatcc tctggcggca
    19261 aaaccagccg ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg
    19321 cgcaccacaa ccagaacaac agcaactctt acagcagcta ctgaccatgc cgtcgggcgt
    19381 ggcagcggta actgctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc
    19441 tcgtattgcg ggtagtgcga ttgtcactgc gcccgcaaaa gcggcaacgg atgtactggc
    19501 acaatttgcg ggcgagaagt ttcgctttat tgcgccggat gccttgttag ccagcgatga
    19561 gcaagccgac tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca
    19621 actggtatcg cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac
    19681 cggacgtggt tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact
    19741 gcaacagccg atccgctggg cacagggatg cccgctggaa aaaatggtta gtaatgcact
    19801 ggtttttgac gatgaaaact tcacccatac accacaaggc aatattgtca tttccgcatt
    19861 tgaacagacg ttatggcgaa tcgagccaga aacgccgtta aaggtttatc agttattgtc
    19921 tggtgcgcac taccggactt cgccgctgga tttacgccgc atgatggatg caccagggca
    19981 acatttttta caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga
    20041 gggtggatta tctcaagaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg
    20101 taatctggtg gcccagtcgc tggcggcgca cggcagcaat ccactggcgg cgacattgcg
    20161 tggacggcgg gtcagccgga ttgcagtcca tccggcgcgt cagcgcgaag gcgttgggca
    20221 acagctcatt gccagcgctt tgcaatatag gcctggcctc gactatcttt cggtgagttt
    20281 tggttacacc ggggagttat ggcgtttctg gcaacgctgc ggttttgtgc tggtgcgaat
    20341 gggtaatcat cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag
    20401 tgatgcgggt aaacaactgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc
    20461 tctcgcgcaa tggaatggcg aaacgctccc tgttgatcca ctaaacgata tcgtgctttc
    20521 tgacgacgac tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc
    20581 gttaggttgc ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg
    20641 gcgtttacag caaaacgcca gtgacgcgca gttatgtacc acacttaaac tttcaggccg
    20701 taagctgtta ctggttcgcc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga
    20761 tgttcgcact gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct
    20821 tcaattttat ctcatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg
    20881 agttcgccat gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt
    20941 tgttactgct tttccacggt gtcggcgata acccggtggc aatgggggaa atcggcagct
    21001 ggtttgcgcc gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg
    21061 gtaatccggc ggggcgtcag tggttttcgg tgcagggtat tacggaagat aatcgccagg
    21121 cgcgtgtgga tgccatcatg ccgacgttta ttgagacggt gcgctactgg cagaaacaga
    21181 gcggagtagg ggctaacgcc acggcgctaa ttggtttctc acaaggcgcg attatggcgc
    21241 tggagagcat taaagccgaa ccgggccttg cttcgcgcgt cattgccttt aacggacgct
    21301 atgcgagcct gccggaaacc gcgtctacag caacgacaat tcacctgatt catggcggtg
    21361 aagatccggt tatcgatctg gcgcatgcgg ttgcggcgca ggaagcgtta atcagtgccg
    21421 gtggtgacgt gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta
    21481 tgcaatttgc cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc
    21541 taagtggcgg taagcctggc gatgatgatg tgattgaaat gatgtaaaag acagattacc
    21601 aggcctgata agacgcgtga tgcgtcgtat caggcatcgt tcaccattgc cggatgcggc
    21661 gttaacgcct tatccggcct acggttgagc acttagctta cttcttcggt tgatcctttt
    21721 tcggccagtc gtcctcatcg tcccacttat cgttgaaatc acgatgcggg ggaagctccg
    21781 gcttgttggc gagaaatttc ttatggtcga cgcgcttgag atctttaatc acgttcagca
    21841 ggacgcctac caaaaagaca atcaccagaa tccaccaata ttcagccagc cagtccatgc
    21901 ttatttcctc ttgcagaacc actcatcacg cgacgagctg ttccatgata cgttgataca
    21961 tacgggcaag tagctgcagg tcggcagcgt tcacacattc attaatttta tgaatagtgg
    22021 cattgaccgg cccgagttcc accacctgcg cccccatgcg ggcgataaag cgcccgtcgg
    22081 acgttccgcc tgtggtcagt agctgcggtt taatttcatt atagtgctca accgcgttaa
    22141 cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa agccaccagt
    22201 ccaccgtata acgcagttga tgtttttcaa gcagggcaag cacctgtgct ttgatcatct
    22261 catcagtcag ttcggtgctg aagcggaagt taaactgcac aaacagttca cccggaataa
    22321 cgttgttact gcccgttccc gcctgaatat tggcaatctg catactggtc gccgggaaga
    22381 attcattgcc ctgatcccac tcaatagcca ctaattcatt aaggaaaggt gctgcgcgat
    22441 gtaccggatt gtcagccaga tgtgggtagg caacatgccc ctgaacgcca tgaatggtta
    22501 ggttgcaggt taacgatccg cgacgaccat ttttcaccac atcacctacc acttcgatac
    22561 tcgacggttc gccgaccagg cagtaatcga gacgctcatt acgtgccatt aacgcttcga
    22621 cgacttttac cgtaccattg tgtgcactgg cttcttcatc agaggtgatc agaaatgcca
    22681 gtcgccctgt atggttggga tgttgtgcga caaaacgttc tgctgctacc accattgccg
    22741 ccagcgagcc tttcatatct gccgcaccgc gcccgaataa catgccgtca cgaatggttg
    22801 gttcaaacgg cggattgatc caacgatcgg cgtcgccagg cggcaccacg tcggtatgcc
    22861 cggcaaaggc caatgtttca ccttgcccac gccatgccca aaaattctgc gtatcggcaa
    22921 aatccatgcg ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac aaagcctggc
    22981 atcctgcgtc atcaggactc agggaagggc ggcgaataag ctgttgtgtc agctcaataa
    23041 ccgggcacga catagactac acctcatgga aaaattgctg ataactggaa tcactgaaac
    23101 ccagcagcat aggcttaccg ggcgcgcaga gcaatggacg tttgataatt gcaggcattt
    23161 cagtcattaa tgccgccgca gaggccgcat cggtgatttt attgcgggtg gtttcgtcca
    23221 gtttacgcca ggttgtacca cgggtgttga gtaacgcttc ccagcctaat tcgttgataa
    23281 aaccgttcaa taattcgctg tccagcccat cgacgcggta atcatgaaaa cgatagtcga
    23341 tgttattggc ttccagccaa cggcgagcct ttttaatggt gtcacaattt ttgataccgt
    23401 aaagtgtaac cataatgaat cctttttcga tttatgagaa taaatactca tttaagggtg
    23461 aaaacatt
//
LOCUS       DAZUGK010000057        23459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZUGK010000057 DAZUGK010000000
VERSION     DAZUGK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.057"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..438
                     /locus_tag="ACHY5W_001972"
     CDS             <1..438
                     /locus_tag="ACHY5W_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4446875.1"
                     /translation="FMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEA
                     KQMLLRNPDSFGRISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAA
                     LLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            438..6752
                     /locus_tag="ACHY5W_001973"
     CDS             438..6752
                     /locus_tag="ACHY5W_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4446876.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWSFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKAPLWAFNTTASAPRVRFGSSSIVQRHVNAQVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADELADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLENLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRK
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            6976..9834
                     /locus_tag="ACHY5W_001974"
     CDS             6976..9834
                     /locus_tag="ACHY5W_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4446877.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTEADTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            9837..14745
                     /locus_tag="ACHY5W_001975"
                     /pseudo
     CDS             9837..14745
                     /locus_tag="ACHY5W_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14745..21086
                     /locus_tag="ACHY5W_001976"
     CDS             14745..21086
                     /locus_tag="ACHY5W_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4446878.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASHAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQDYAIAYSQYPGPEAASALDPQSY
                     STPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYLEVDNMATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGEFWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVVIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21083..23197
                     /locus_tag="ACHY5W_001977"
     CDS             21083..23197
                     /locus_tag="ACHY5W_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4446879.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAPEAVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHHDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         5954 a         5597 c         6293 g         5615 t
ORIGIN      
        1 tttatgaacc atcgtgcaca tatcgatcgt cggctggctc cgattgcgaa tatgttactt
       61 acatatagtg agagcctacc aaccggttgt gatgtattgc caactgttag tcgtgagcag
      121 tttaatgaag ctaaacagat gctattaagg aaccccgaca gctttgggcg tatcagtatc
      181 ttccctaaag aactttggtc tagtgctatt actccagagt taaaatctgt ttttaataag
      241 ctttggattg aagataaata tcactcacgg cttgaaaaac gttttaattt gatgttagtc
      301 gcagcgctgt taacccaaaa agataataac ctgatacatc aactgtctgc gctttttgaa
      361 tttcactatc agcaagcccc acagcaatta ggggtaatct atcaatatta ttttgaacaa
      421 gcaggagtat gtcattgatg gacgttcagc aactgcatta tgcgccactt ctaaatcagc
      481 ttcatcagcg tgcggtgagg tcggtggtaa gtcagttggc attgcggagc aagtcgttga
      541 ctcattatct ggaatcacaa tattttcagc ctcccggtca ggaggggtca ctgcttgcag
      601 acccggtatt cgaaagtaca ttcggctgga tgcctgcgga tgaaacaatg gaggatttgc
      661 gggacaagct gatctccagc ccgctggttg atgcattgag caaagcgcca gtgccatttc
      721 gtcatcaact tactgcgtgg cgaacgattc tggaagacaa aaaatccttg ttagtgagca
      781 gcggtacggg atccgggaaa acagaatgct ttatggtacc ggttctggaa gatcttattc
      841 gtcagcaaaa atcgacccgc cagtcactaa cagggacaca agctattttc ttgtatccgt
      901 taaatgcatt aattaacagc caacaggagc ggcttcgcga atggacgcgc ggatttaagg
      961 ggaaaatacg cttcgcgcta tataatggcg aaacccggca taccaaatac gaaattcagg
     1021 aagatcagtt gaaagtacct gaacaagctt tgagtcggga agccatttat gaagcaccac
     1081 cttccatcat ggtgacgaat accaccatgc tggaatatat gctgattcgt cgtaaagatg
     1141 ccccaatcat cgaaaagtct caggggatgt taaagtatat cgtcctggat gaagcccata
     1201 gttatattgg ttctcaggct gctgagttag cgctgctttt acgtcgtgtt atgcaggctt
     1261 tcaatgttgg acccggcaca gataaacctg tgcagattat cgcgacctct gccactattg
     1321 gtgaagactc tcctgaaggt aataaggagc ttgctaaatt tgtcgctgat ctcgcgggcg
     1381 ttacagaacg tgatgtcaaa gtggttcgcg gttatcgaca aataccgcgg gtatcggagt
     1441 cgctgattcg tcatgaatac cccacatctt taacttctct taaatccctg agccctcagg
     1501 atctttacca acagctttgt cattaccgtg tggcccagca attgcgtcag gcgttaactc
     1561 atcccggacg gcaggcagta cgtttgagtg agttgctcaa tgttgccaga agaacctggc
     1621 cagatattaa tcaccgtgag ctactgcaac tcctcgatct aatggcaaga tcgcgagaag
     1681 gtgaactggc ttttacgcca ttacgtatgc atgggtttat cagaacactg gcgggtctat
     1741 gggcatgttc aaacaaacaa tgctcacata aagcacatga attaaatcag agtgactggt
     1801 catttggtca ggtatggttt gagcagcgcc aatattgtga ctgtggagcg ccggtatttg
     1861 aagttttacg ttgctccggg tgtggttcag cttatttgtc tgccaaagag gagatgcgcg
     1921 gtgatggaac gaactggttg gtagcgcaac cagccgctgc cgaagtggat gagtttgctc
     1981 ttgatgtaga tgtttattct gaagatgaag acaacgacga attagacaat aactctcaat
     2041 ttgatcgtct gattgcgagc gatggtgaaa aatatatcat ttcactggaa gaaacgacgg
     2101 cggggaaaat tgatagtgaa gcgcaaaagc actatgaaat aaacctgtta agaccggaat
     2161 ctcgaggtga gggacgcaat aactcctttg cttgtgtttg ctgtggtgac actcaacgga
     2221 aaaataatcc tttattccgt ccgcttcggc tcggcgcgcc ctttttttta aatgaaatta
     2281 ttcctactct gctggagttt tctccattac cgcaaacaag agaagagcag ttaggaccgt
     2341 ttaatggtcg acgcttgctc acctttacgg attcgcgtca gggtaccgct cgcatttcag
     2401 cccgcttaca gcaggatgcc gataaagcga cgttgcgagc tctttgttac caggaactgg
     2461 caaatattac agagccttca gcaaaactca gcccggcaca ctgtgcactg ttagagaaag
     2521 ttattagcca gttttctgtc ctccctttaa aaaattccat cgacgcgctg aaaaaagcga
     2581 tggctgcgaa tgaggagctt agcccgacgg acattcaggc acttaaaatt attgagccga
     2641 tggtgagttc cttaagcacg gagcatgctg aagcgatgca aatacttcag gctaactctg
     2701 gcaatcagca gaagaaaacc atgtcatggc aaacgctgtc tgaacagctg gttggcagtg
     2761 tggaccttgc cgatagaatg agaggcagtt ttaaagaatt gtctgggctt gatcttacta
     2821 aacaacagtt tgcagatttt tgtttataca gcgagtttgg tcgccgccct aaaaatgcct
     2881 ggaccctcga aagtctgggt ctggtagcca ttcactatcc attcattgat aaagttacaa
     2941 cctgcccgca ggactggaag acattattac ccgatcccga taagcaatta gcggaatggc
     3001 gtaatttatt gaagattact cttgatttct ttatccgtga aaacagtgcc gttttttacg
     3061 aaaacgagca ataccctcgc tggatggggg cacgtttccc ggtaaaaatg ctccaggggc
     3121 cagataaaaa aaacaaaggg cagaaacgtg atcaactttg gccacaaata cgcgatcgcc
     3181 attataaccg ggtcattaag ctattaatcg cagtctttcc ggcaatacaa cctgagcagg
     3241 ctcactggaa atcgttagtt aaccacttaa tgattgaggt ctgggatgca attcgaccct
     3301 gcttacgtca gtttgagtcg ggttatcagc tggatattaa acaacaagct gaattttaca
     3361 gtccaaagca agtgtggcgt tgcccctata ctcgacgtgc gttagacgtc acgttgctgg
     3421 gatactctcc gtatttgccg ggtagcaaag aaattgcacc tgaaaaagca gtgttgattg
     3481 aaatgcctga gcttcctgta cgtcactggc gtttgtctgg tggtggagaa atcgcccgag
     3541 aagaacgtct ggagtggctg gaaagtaatg cgctgattca acatgcccgc gaggaaggat
     3601 tatggtctac tcgcagcgac cgactggccc tgaaagatag ctggtatcgt ctggaggaac
     3661 actctgcgca gagaacgcct gagcagaacc agtttaatga aaaacaattt aagtcaggca
     3721 aagttaatgt gctgaactgt tccacgacca tggagatggg ggtggatatt ggtggtatga
     3781 gtttggtggc aatgaacaac gttcctccag cgccggcaaa ttatttgcaa cgtgcaggtc
     3841 gcgcggggcg tcgtggagag agtgcatctg ctgccattac gttatgtaaa aacacggcgc
     3901 atggaatgga ggtattcaaa gcccccctct gggcgttcaa cactactgcc agtgcgccac
     3961 gggttcgttt tgggagcagt agcattgttc aacgacatgt taatgcccag gttttaggtc
     4021 tgtttttacg agctgaagtt cctgatgcga caaaactgag ttgcaaatgg ttctttgaag
     4081 gtgatgagag ccagtgtctt cgttttcttc attggttaaa tcaccaggcc gatgagctgg
     4141 cggataaact taaacgcctt actcagggaa ccgtcctgat gtcattgacg gcaacccagc
     4201 tcttgacccg cacacaagca atgatgcagc aggttgatat ccgctggaga agccaacttg
     4261 cgatactgtt ggaaaatatc gaagcgttga aggcggataa cagcgcgtgg gaagaaacgc
     4321 cagcaggtaa agccatcgct tatcagcttc gtgattatcg cggtgcatat ctgttttcta
     4381 aactcattag tgaagcgttc ctgccggggc atggtttccc ggttggcgta gtcaatttca
     4441 attatctgac ggcagatgag ctggaaaaac gtcgcgcaat aaaagccact caggctgacc
     4501 ctaatgaagg gggagaaagc ttttctcgtc gtattgaaaa attgcccagc cgtgatttgc
     4561 ctacagcatt acgcgagtat gcgcctggtg cagatgtggt attgggcgga aaagtatatc
     4621 gttcctcggg cattatgctt ggcaaggtgc ttgccagcgg gcaggagctg tcaggcgatc
     4681 atcatattcc gtggttctgg cattgccgta aatgtggtgc gggggcaacc agtactaccc
     4741 atcctgtcga atgtagtcac tgcaaagcgg atatacagca acttgatgtg aagcgttatt
     4801 tacagcctgt tggttttgcg acagatattc gttatcaggc acataatgat gtatccatgc
     4861 ctgcacaact tccctggaaa gatccacgtg ttcttgtacc gtcttcggtg tgggtttctt
     4921 tacccgatgc tgggctagga cgttaccgtt ttagccatag tggggaattg ttccacttca
     4981 gtgaagggga atttgggcac ggctacgcaa tttgcctctc atgtggtcgg gcagaatctc
     5041 aaactcaacc ccagcgtacc cctgaaaatc taaagaaccc agagcgggaa aatacgcatt
     5101 acctgttgcg agggggcagt aacgatcgtc aggggagcaa taaattgtgc cacggtcatg
     5161 tgcataaaga tctgtggttg gggtatagca gtcgtacaga tatggttgag ctgcaactta
     5221 atgatgataa tggcctgttg atccgtgatg aggttgccgc gcgttccctt gcggtcgctt
     5281 tgcgtgaagg gttagcgcat aaattgggta tcgaaaatac tgagctgggc gtgacgacgc
     5341 agcaagcgcg cgatatcaat ggctataccg gatattccat cttcatatac gacaataatg
     5401 cgggtggtgc aggttatgca gtgcaattga tcgaccattg ggctgatgtc tttaattacg
     5461 ccagaaaatt gctcgattgc agttgcgata aattttgtca ccactgcttg ctcagctacg
     5521 acagtcagca ttatgtcaac agattagatc gtcaccatgc attaaccctg cttaccaatg
     5581 tacggttgca gcgacttaac ctcgcgccag aatatcagta cttcggcgat ggtagccggg
     5641 ttgaaactaa ccctctgagt ttacgtattg cgcaatgcct gaacagcgag atttatgact
     5701 cctgctcgct ggttcttgcg gggccgcagg aacagtggga ctttgcccaa tggccattat
     5761 ttaaagagtt gctccagttt gcctcctccg gtgggaatgt tgagttgctg gtggccacgc
     5821 cgctggaaaa cttaactgat agctccagac atcagctttc cgcgttggcg gcgatgccgg
     5881 gaggacgctt acaagtgaaa tccattgcca ctgcgcaact catgcaagga aaggggcgct
     5941 ggcttgcgca ggtgactcgc aaagggcaaa gccaacagtg ggccgcagac gacagcgcta
     6001 ccgttgcccc tggtgaatta tggggacaaa gtgcgtcttc accagtagtg acacttaaag
     6061 gaacgtccgg aaaaaccttc tcgggtcaga ccttaagtgc agaagattta ctgcccgcct
     6121 tacccaccgg ggcggtgcgc atcaatctct gtgaacaact ggacgggccg ctggaaggtt
     6181 ttggttcgcg tttctggtcg ttagtcacgc aacagcacgc tggctggaag caggcattta
     6241 cccgccacaa agagataacg catgttgagt acagcgatcg ttatcttaat agcccattca
     6301 cggcgcgttt gttaggtgaa atcttaaccg agctggttga acagggaatg gcggaacgcg
     6361 ccagcctcac tgtttgcgtc aaaaaattgg attacaactc tcgccaacac gacgctctct
     6421 ataacgcgtg gcttaatgag gaagatcgtc agcaggttgt gaccacttta cttgaagaag
     6481 gatatctggg gcctgcgtgg ccgggagcaa ttagctggtt aacgggggat aaccagagta
     6541 cggagcatgg acgagaactg acagtcacct tcagcgatgg cagtcagcat tatgtcctgc
     6601 tcgacatggg gcttagctac tggcgctgta ttgaagatac cttctttgat tttgccctgc
     6661 gagtaccgca gcaagttgaa cggctggcaa acacgcgagc gcgagctgtt gccccgggta
     6721 atgaccttag gagttatatt atcgctggat aaataattgc tcccctgggg atatcttcca
     6781 ggggagttga cccagctcac tttttaataa tttttgtttg aaaatcatgc gcaatttatg
     6841 cgcattccct cccattttgt cactttgccg ggggccttca tgatatcttt aaggccctct
     6901 tttttatcgc agtgtgccgc gccacatttt tcaggatgac gttaccgcca ctgccccaaa
     6961 tagacagaaa aaccgatgaa taagcaaaat tatgcacctg gtatgcgggt ggttattcgt
     7021 gatgccgaat ggcgtattcg ccgggcggat gacagtggtg atggtgggta tttgctgacc
     7081 tgcgatggta tttcagagct tgtgcgcggt aaagaagggt tatttctgac caaactggaa
     7141 caaaaagtag agatcctgga tcccgcaaaa acgcatttgg tggaagatga atccgccaat
     7201 tatcaagcgg cgcagttgta tatcgaaagt cagttacgcc aacgcgtacc gacagacagt
     7261 aaagtccatt ttggtcatct ggcggcgatg gattccatgc cgtttcagct cgatcctacg
     7321 cggatggcgc tggcacaacc gcgtcagcgg atactcattg ccgacgctgt cggtctgggt
     7381 aaaacgctgg aagcgggtat tctggtttcg gagctgatcc gtcgtgggcg cggaaaacgt
     7441 attctggttc tggcggtcaa atcgatgctg acacaattcc agaaggagtt ctggagccgt
     7501 tttgcgatcc cattgacccg cctggattct gctggtttac aaaaggtgcg taaccgcatc
     7561 ccaaccaacc acaacccgtt ccactatttt gacaagacca ttatctccat cgatacgctg
     7621 aaacaagaca ttgaatatcg tcaccatctg gaaaatgcct ggtgggatat tattgtcatt
     7681 gatgaggccc ataacgtcgc agaacgtgga accagttcgt tacgcagtaa actggcaaaa
     7741 ctgctcgccg ggcgtagcga tacgctgatt atgctttcag caacgccgca tgatggtaag
     7801 gctgaaagtt ttgccagcct gatgaacatg cttgacccga cggcgatcgc caacccgaaa
     7861 gagtatgaat acgccgactt tgccgataaa aatctggttg tccgtcgctt caaaaaagac
     7921 gtgaaagatc agatgtccgg cgagttcccc gagcgcaata ttgtcaaatt gactcgcctg
     7981 gcatccggtg ccgaagaaga agcataccgt cgtctggttg agagccagtt ccgtgatgat
     8041 gacgatgaac aggcccagtc taacaaaggt cgtctgttca agataaccct cgaaaaagcg
     8101 ctattttcca gcccgatggc gtgtgccagc gtcgtggcga atcgtctgaa gcgtcttgag
     8161 agccgtaaag atcataatag ccagagccag atcaacgaac tggagtcatt gctgttggcg
     8221 ctgaataaca ttgatgccag ccagtttagc aaataccagt tgctgctcga caccatccga
     8281 aaagacctcg cctggaaagc caataacacg gaagatcgcc tggtgatctt caccgaaagt
     8341 attaaaacgc tggagttcct cgaacagcaa ctgcgagcgg atctgaaatt gaaagatgac
     8401 cagatcgcca cgctgcgcgg cgatcagggc gatacggtat tgatggaaac cgtagaagcc
     8461 tttggtaaaa cgcagtcgcc gttgcgtctt ctggtttgtt cggatgtggc atctgaaggg
     8521 atcaacttgc atcaccttag ccacaaaatg attcactttg atattccgtg gtcgttgatg
     8581 gtattccagc aacgtaacgg gcgtattgac cgctatgggc aaaaacatca gccccaaatc
     8641 cgctatctgc ttactgaggc cagcgaacca cagatcaatg gcgatatgcg cgtactggaa
     8701 gtgttgatca acaaagacga acaggcgcag aagaacattg gggacagttc ggagtttacc
     8761 ggcaaattta cccaggaaga ggaagaagag caggttgcag agttcatgat gcaggatgat
     8821 ggtgccagcc tgtttgatca actgctgaac agcaacgtct cggaaagcgc cgaacacgat
     8881 ctgtttggcg aaatatgcag tgcggtttcc agcgatgcct caatggtcac cgaagcagac
     8941 accagcttgt ttgccagcga acaggcgtac tgcgaaagag cattgggtta cctgaaagcc
     9001 agtggtcaaa ctatccagta tgaaaccttg cctgataata ccttgtcgct ggtggcaccg
     9061 gaggagttac gccgccgctt caaccagcta ccgcctgaaa tcgccccgga gaactggcag
     9121 ctctatttaa gtcaggataa aaccgtcatc accgacgcga ttgcacgcgc tcgcggtgag
     9181 caacatgcct ggcccgatgt gcagtatctc tggcaaatca acccggtagt gcagtggctg
     9241 gacgataaaa tctcttcggc ttttggtcgt catcaggcac cagttatccg tctgccatac
     9301 ctgcttgaac ccgatgaaga tcacttcatt ctttccgggt tattcccgaa ccgtaaatca
     9361 catccgatgg tgaacccgtg gatagtggtg agctttaacc gtgaatcgct aatcggcagt
     9421 cagccattcg ctgagttttt acaacgtcat ccgcagttga gtaacaagct gactaacagc
     9481 ggcggtaaag atcgtaatca ccaacgccag caggatttac ttgaagcggc tattgctcat
     9541 gcccgggagg tattcatcca tgatcggaat gcgtttgaaa cgcacattaa ccagcaactg
     9601 aatgagcatc tgcaaaagct ggacgttttg cgtgggcggc agttgagcca acttgagctg
     9661 gattttgccg ataataaaca gcaattgtca gtcaagcaga gccgtaaaga gcagagacaa
     9721 cgcgaaatcg aacacaattt tgacagctat atcgaatgga ttgaggacac catgacgact
     9781 gaaaaagaac cctacattca ggtaattgct gttatcaccg gagcggaggg ttaatcatgg
     9841 cgctggtcgg tattaataac gaaaacgaat tttactctaa ccactatttg ggtgaggttt
     9901 tcaccagtga tatccgcgat gtgctggaac cctggatagc caaggaaaat gcagcgcgtg
     9961 aagcggagcg tgccgctcgt gaacagggca aagacgtgga gccgggatac cgcgctccgt
    10021 ggaaccagtt taacagtctg gcgactgagt ttttccgcaa acttgccgag cacgaaaaac
    10081 agcgtcagat cccgcagcgt ctggccgatc aacgtaatcg ctggcagcca ttgttaaagg
    10141 cgctgggcta cgaaattacg ccacagatcc aaatgctgga tgacgataca ccgctgccgg
    10201 tactggcgcg ttacaacagc actgacggta gcccgtggct gtggattgtt gaagcacacg
    10261 atcaggaaga aggaacgctg gatccgctgg cgctctcctt actgaccgcg caattcccgg
    10321 cggataccga caaacataag cgcgacagcc tgcgcaaaaa agccaacggt gaatatcgca
    10381 gctggcagga tctgctctct acggcggtct tcacccaaaa tgaaccgccg cgttttgtgc
    10441 tgctgctcgg taaccgtcag ctcttgttgt tggaccgtac taagtgggcg caaaaccgtc
    10501 tgctacgttt tgattttgaa gagattttaa gccgtcgtga aacggatacg ctgaaagcga
    10561 ctgcggtgtt gttacataaa gattctctgc tgccgggcag tggggcacct tatcttgact
    10621 cgctggatga caattcgcac aaacatgcgt ttggtgtttc ggaagatctg aaatatgccc
    10681 tgcgcgaaag cattgagttg ctgggcaacg aagcgatgca ttatctgatc gaccgtggcc
    10741 tggcaaacta taccggtaac cgtgcggtgg acccggatga actgagccgc gaatgtctgc
    10801 gttacatgta ccgcctgctg ttcctgttct acattgaagc gcgcccggag ctgggttatg
    10861 cgccaatgac tgccaaaact tatctacagg gctacagcct ggaaacgttg cgcgatctgg
    10921 agatgatccc gctgaccagc gaagaagatc gcaacgggcg ctacttccac gacagcctga
    10981 atatgctgtt taaactggtg cgcgaaggct acaacggcgg cgtgaaaatg cagagtgacc
    11041 tggagagcgg cgaccggatc accatccata gtcatcagtt cagcgtcccg cgtctggaaa
    11101 gtcatctgtt tgatgccaac aacacgcgca ttcttaaccg cgtggtattc cgtaacgaaa
    11161 ccctgcaaca gattatccag gcgatgtcgt taagccgccc ggccaaaggg cgctttaacc
    11221 gccgcggacg tatttcttat cgccagttgg gtatcaacca gttgggtgcg gtgtatgagg
    11281 cgctgctctc ctatcgtgga ttcttcgcca gcgaagacct ctacgaggtg aagaaagccg
    11341 gggaagagtt taacgagctg gagacaggtt acttcgtcag taaggatgag attggcaaat
    11401 accacgaaga cgagaaggtc tacgagaaag acggcagtct gcgcattcac cgcaaaggca
    11461 gctttatcta ccgtatggcc gggcgcgacc gtgagaaatc tgcctcttat tacaccccgg
    11521 aagtgctgac ccgctcactg gttaaatatg ccctgaaaga actgtttaag gagcagattg
    11581 atcccattag cgatccgcac gccaaagctg atgccatctt aaacctcacc gtgtgcgaac
    11641 cggcgatggg cagcgcggcg ttccttaacg aagccatcaa ccagctggcg gaagcgtatc
    11701 tgttccacaa gcagcaggcg gaaggtcgcc gtattccgca ggatcgttac acccaggagt
    11761 tacagcgggt gaaaatgtac attgccgaca acaacgtttt cggcgtggac ttaaacccgg
    11821 tggcggtgga actggcggaa gtgtcactgt ggttgaacgc catcagtggc gatgcctttg
    11881 taccgtggtt tggttaccag ctgcactgcg gtaactcact ggtgggcgcg cgccgtcagg
    11941 tgttcaacaa gagtgaactg acctacaaaa aagccaaaga tccgagctgg cttaacagcg
    12001 agccggtcga actggcgatg aacacgccgc gtgaagagac gcagattttc cacttcctgc
    12061 tgcccgacgg cggtatggct aactacagcg ataaaactgt taagcagcgt tatccggatg
    12121 acttcaaagc cctggacagc tggcgcaaag agtttattaa aagctttgcc gggcatgaga
    12181 ttgctgatgt gcagcgtatc agcgaaaagg tggaagctct gtggaacact tatcgccaac
    12241 aacttaaagc agaacgtctg aaaaccgccg acagctaccc ggtgtggccg gcagaaaaca
    12301 gcgagcagac gcgttcttcg ctgagcagta aagatgaaac ctttagcggt cgtcttgaag
    12361 ataacagcgc ctaccagaag ctgcgttggg taatggacta ctggtgcgcg ctgtggttct
    12421 ggccgatcga caaagccgat gagctaccgg atcgcggcac ctggttgttt gagattgaaa
    12481 ccctgctcga cgggattgta atcacggaaa aagtcactga agttgcggag cacaccaccg
    12541 gcgatctgtt tgccgaagaa ggcctgctgc gagaagagtc ttcgctgttt tccgttgctg
    12601 gtcgtctgaa aaccgaggtg ttgttccgtc atttgccgcg tctggcgatt gtcgatgccc
    12661 tgagaaagca gcaccgtttc ttccaccggg atctggagtt ctgcgacctg tttgccgagc
    12721 gcggcggttt tgacctgatg ctcggaaacc cgccgtggct gaaagtggaa tggcaggaag
    12781 cgggcgtgct gggtgattac gagccggaat ttgtgctgcg taagctgagc gcctcgaagc
    12841 tggcaacgtt gcgtgttgat acctttaacc agatcccggc actggaagcg gcctggcgca
    12901 gcgagtatga aggctgtgaa gggatgcaaa acttcctcaa tgcgcagcag aactacccgg
    12961 tactagcagg acaaaaagcg aatttgtata aatgttttct acctctgggg tggcgcttag
    13021 gggcgcagaa aggtgtcgta gggttcctgc atccggaagg aatttatgaa gacccgaaag
    13081 ggggaaaact gcgtggggcg gtctatccaa ggttaagagc tcattttcag tttcaaaatc
    13141 agcatatgct tttccctatc ggtgacaggg ttaagtttag tattaatatc tacaccgcgt
    13201 ttgtaagtga tcatattggc ttcattagta ttgcaaactt attctcggca aaaacgattg
    13261 aactgtgttt gtcgcatgat ggaactggag aagtgggtgg tattaaagat gacgaaaccg
    13321 actcatggaa cgaatcagga caccaaagcc gcatattgaa agtatcgtta gagcggcttt
    13381 ccttgtttgc taatttgtat gatgagctag gtatacctgc gattgaggcc agattacctg
    13441 cttcatgcgg atcacttggt gtctgtgctt gagaaatttg ctaagcagcc aactagattg
    13501 agtgacttag aaaaagacta ttattcaacc caacattgga acgaagttaa tgctcaggat
    13561 gatgggataa tcagacgcga aacccaattc ccggaagacg catcccaatg gattttgtct
    13621 ggtccgcact tctatgttgg aacaccgctt tataaaacgc cgagagaaat ttgcacagag
    13681 aaaggacatt atgactgcct ggacttgcta actctgcccg acgactatct gccgcgcact
    13741 aactatattc cagcatgtga tgcacaggag tatgcaaagc gcaccccacg ggtttcgtgg
    13801 aaagaacagg atgaagacga gccgaggaag gtgacggatt attatcgtca tataaatcga
    13861 agagccattg gttgttcttc ggagcgaact gctataggtg caattatacc caaagatgtc
    13921 gctcatatag atgggggatt ctcattaact ttcaaagacc ctaaacaact ggtgattttt
    13981 aataccagct tcaattcctt accttttgac tttgtaatta aaacaacggg taaaagcgat
    14041 tgtcgggcgg aattagtaaa aattttacca atgctttcag atatagggac aaggctattt
    14101 gctcggggct tagttaataa tgcaataaca gcgcattact ccgatctctg gcaatcctgc
    14161 tacaccccag acttcaacac ccagcgttgg agccgcgatc tcccgctgct gccccaggat
    14221 ttcttcgcca atctgacccc agagtggcag cgtaactgcg ccttacgctc cgactacagc
    14281 cgccgtcagg cgctggtgga aatcgacgta ctggtggcgc aggcgctggg gttaaccctc
    14341 gaagagctgc ttaccattta ccgtgtacag ttcccggtta tgcgccagta cgaagcggac
    14401 acctggtacg atcaaaacgg tcgtattatc tttaccccaa gcaaagggct ggtgggcgtt
    14461 ggcttgccgc gtaccgcgcg taaagctgac ctgaaaaacg gctttgtctt taacgtcgac
    14521 agcccggact ggaccggcgg tgactgcacc gatcaagcca tcggctggga tgatgtcaaa
    14581 catcttaaaa ctggtaccgt cagcgtcacc tttgatgact atacccgcag cgacgaaggc
    14641 gagcgccgta ccgtcacctg gcaggcaccg tttatcaagt cggatcgcga agacgactac
    14701 aaagtggcct gggcgttctt tgcacaagat aaggagagcg cctgatgctg ccgtccgttg
    14761 tcagtcggca ggtcgccgac agcgttgccg cctttttacg ggcggcgttc ccgctaaaca
    14821 gcccgctgtt taacggtgag aataacgata atgtctcaat gctggagcaa tttctggccc
    14881 agcccgaaac gctgctgaaa ggaccatacc tttccgccca gttgccgttt cgtaaaagtg
    14941 acttaccact gaatttcttc cctaatctga cgctgccgtt cccgccgcac gcgcatcagg
    15001 cccgggcatt tcagcgtctg ggaagcgacg cgccacagcc gacactggtc gctaccggta
    15061 ccggctccgg taaaacagaa tgctttatgt tcccgctgct caaccactgc gcgggcgcgt
    15121 cacatgccgg ggttaaagcg attatcatct acccgatgaa cgccctggca accgatcagg
    15181 ccagccgttt cgccaaaacc atcgccagcg atccacaact gcatggcaag gtgaccgtcg
    15241 gcttgtttgt cggcgacagc gaaatcgaac ccagcaagaa aatgtcggca gaaaaggtca
    15301 ttacctgcaa gcacacgctg cgcgaaagcc cgccggacat cctgttgacc aactacaaaa
    15361 tgctcgacta cctgctgatg cgccctggcg atcagccgct ctggcgttac aaccagcccg
    15421 gttcattgcg ttacctggtc gtggatgaat tgcatacgtt tgatggcgcg caagggtcgg
    15481 atctggcctg tctggtgcga cgtctgaagc atcacatagg tgtggataac caacgattcg
    15541 cctgcgtcgg gacatcagct accgtcggcg acgaactggg gcaactgctc gattacgcaa
    15601 aaacaatctt tgaccagccg tttaccgatg acgccgttat tcgcgaagat cgctacacgg
    15661 cagcggagta cttgcaggat tatgccattg cgtacagtca gtatccgggg ccggaggccg
    15721 cttcggccct ggacccgcag agttactcta ccccggtagc gtatctcaac gggcaaatcc
    15781 ctttgtggtt ccccgacagc gatttgcaac tcccggacga tctggagagc gacgacgggc
    15841 gtgaaaagcg tattgcgctg gggtcactgt tacgccgcca cagcgtgatg catgtcttac
    15901 tccatgatct gcaaggcggg atcctctctg agcgagagtg tctggaaaat ctccaggtca
    15961 tgctggcaga aagcgccgac catgcccgac gcgtgctgca aagcattctg gcgctgatcg
    16021 cgcaggcacg tctcgaagta ccggaaacgg aacacgatcg tcaaaaacgt ctccaggcga
    16081 agaaagcgcg tccggtgctg ccgtttgtgc aactgcgctc ccagttgtgg cttcgtgaga
    16141 tgcgtcgtct ggtcgccagc gtggcgaaaa cgccgcgact ggtgtttgcc gatgatgtgg
    16201 cggtggaaga ccgcgaacac tatttgccgg ttatccactg ccgtgactgc catgcaaccg
    16261 gctgggccag cctgcgtcat gggcaaagcg ttcagctgga aacagacctc gacggcattt
    16321 atcgccagtt ctttgaaaaa gggcgctcca tcgtcctcgc tttcccggat aataacgaaa
    16381 aagcggtcag tggcgcacac cagaaactgt gtcctacctg tctgaaactt aaccgacagt
    16441 ccagcgtcca gtgtggacat tgcggtcata cggaactgtt gcaggtactg atcccggata
    16501 tgctcaaaga gcgtaaggac gagctggagt ttgctaatga atgcccgtac tgcaacagca
    16561 aacaggggat ttcgatcctg gggtcacggg ctgccagtct gtcggcggtg atgattcacc
    16621 agctttatgg cagccagttc aacgaacata aaaagctgat tacgttttct gactcggtac
    16681 aagacgccgc gcatcgtgcc agcttcttta ctgcccgaac ctggccgctg atgattcgtg
    16741 ggcaaatcgc cagtgttctt ggcgaaaaag agacattacc gctggatgcg ttcgcggcgc
    16801 gtgtttgcca acaaacgcgt gaacgttcac cggacgatgc tcattttgca gcgaacttca
    16861 tcgcaccaaa tatgcagtgg ctgaatgatt atcagacgct gaaaaatgaa ggcaaactgg
    16921 cggagaactc cgatctgccg gagctggttt ccctgcgcct cgactgggag gtgtacagcg
    16981 aatttaccct gcgcgcgcgt atcggacgta cgctggaagc caccggttat gccgttgccg
    17041 gagtggataa agatcgcttt aatccggcaa ttaccgccct gcatcaacag ctttgtgaag
    17101 agctgggtga tgagatgtcc ggtgtgagcg tcgagcaggt agcgcacctg gcgctggggg
    17161 ttatctggta tcaacgccag gccggggccg ttatgcatcc ggcgttcgaa agttatcgcc
    17221 gtgatggcac gacctttatg atgacgcaaa aagagcgtac atcgcgctat atgccttcta
    17281 tcggtccgaa aacgcgtaaa ccggcttatg cgtcgtttga aaaaattaat ggtttccatc
    17341 gcctgattgg cgcgaagtct aaccccagtt ggtatcagca ttggatcaat cgcacgctga
    17401 gtaacggcaa taatctgttt atcagtagcg ttgcggaaac agtgttacga cgcctgttta
    17461 cggcgttgaa aatagccgga gtagtaaaag attttgacac caaagggcgt gaagcctggg
    17521 gactggtgcc ttcggcgctg gtggtgagcc gtgacgtcgt tcagcttaat tgctcatgtt
    17581 gccgtgaggt tgtccgggct ccggccgatc aaggctggca gtggcgtaat gcgccttgtc
    17641 tctctttacg ttgtgaaggt cgttacctag aagtcgacaa tatggcgacc tggcgttggg
    17701 agaacatgga catcgcgcgc gtgcagggcg cagaacacac ggggctgctt agccgtgaag
    17761 atcgcgaagc caccgaaaaa tcgttctacc gcggcaacca accgtggaat atcaacctgt
    17821 tgtcggcaac accgacgctg gagatgggta ttgacgttgg ggatctttcc accgttttac
    17881 tctgttctgt cccacctgct caggccaact acttgcaacg tattggtcgc gccggtcgta
    17941 aagatggtaa cgcgttaaac attaccgtgg cagaagggaa cccgcacgat cagtttttct
    18001 ttgaagaacc actggagatg atgcagggcc aggtgcaggc tcctggcgtc ttcctcaatg
    18061 caacggctat tctcgaacgc cagctggcag ctttttgtat ggataactgg gtgaaaaccg
    18121 gtgtaccaga atccgctatc tgtaaaaacg tcaaacagat gctggatgag ctggagttcg
    18181 ggcgtaaatc gggttttccg tataacttac tgcgatatat cgagcagcat catgcggaga
    18241 ttgctgcgca atttacggca atcttccccg atcttgccgc agaaactcgc caacagctgt
    18301 tgtcttatct tcagggcgcg ccaggacaac gctcgctggt gcagcgtatc gaagaagcgt
    18361 taaaactgct ggtggaagat cgcaaatctt tgcgctcacg catcgataaa ctcaagcgca
    18421 gtatcgataa actggagaac gcgccgcgcg atcaaaactt tgatagcgat atgcgcgagt
    18481 taacttcgga acggcaggcg ctgatggcgc tggtcaatca aatcaacaat aagcaaacgc
    18541 tgaatttcct cacggatgaa ggtctgctgc ctaactacgc cttcccggaa gccggtatta
    18601 cgttacgttc tgtgctctgg cggcgtaaag aaggcgggga agcacgtgaa tatcaaaaca
    18661 ccactttcga atatgaacgc ccggcatcaa cggcgctggc tgagctggct ccgctgaata
    18721 atttctatgc cggtgggcat aaagttgaaa ttgagcagat agacctgaag gtctctgagc
    18781 cggagaactg gcgtatctgt agccactgta actacagcga aaatatcgac cagacgggcg
    18841 accagcataa atattgcccg aaatgtggca caccggggtg ggccgatgcc ggacaaaaaa
    18901 ccacgctgct gaaacttcgc caggtttatg cccgcgccag cgcacgtgat agccagatca
    18961 gcgacgagag cgacagccgt gaacctgctt tcttccagcg tcagctattg gttagtttcg
    19021 aaaaagaaga tgtatcggcg gcatacgcga ttgaggaagg cgaagttccg tttggttttg
    19081 aatttttaag caaagtcacc ctgcgggata tcaacttcgg caaaatggcg gatgatgcca
    19141 atgagttgat gattgccgga gaagcgaaga agcgaaccgg gtttaaagtt tgcctcgggt
    19201 gcggcatggt gcagcgtcct cgcgatcatg aaccacgtca tgatttgagc tgcaaatatc
    19261 gcacggaacc ggaaaaggcg aagttcgaag attatctcta cctctatcgc cagctggagt
    19321 cagaagcgtt gcgtattctg ctcccggtaa gcagttacag taatgaccgt gtcgtggagg
    19381 cttctcttgg ggcggcgatc cagctcggtc ttaaacatta cttcaagggg aatgtcgatc
    19441 acctgaaagg cgttgtgtac cgcgagccgg aaaatgaagg cgaattctgg cggcagtatc
    19501 tggtgattta cgataccgtc ccggggggga ctggttctct taaagaactg atgcgcactc
    19561 ccgacaattt gctgaaatta cttgaactgg cctatcaggc aatagtggaa tgcagttgca
    19621 acaatgatac gcataaagat ggttgttatc gttgcgttta cgcttaccgt gaccgtgggc
    19681 gcatgaagta tgtctcccgc gatcaggcgc gtctcttgct ggcgaaaatt ttgcaggcaa
    19741 gagattcgat acgggtaatt gattccatca aaaacatttc cctcgaagcc atgatgggca
    19801 gtgagctgga aaaacggttt attagctgcc tgcaagaaaa taaaaacctg attgtcagtc
    19861 gcagttacgc gcatcagggc gctgggtgga ttattaacac ccgcagcgaa ccgatgatga
    19921 gctggcatct taaagctcag gtggatttag gcgctaaaga ggcggtgggg atcccttgtc
    19981 gcccggatta cgtgctatat ccgcttatgc agtctgatgc cattaaaccg gtggttattt
    20041 tccttgatgg ctttgcgttc cacaaaaata gcgtcgcaga ggatgtgcaa aaacgccagg
    20101 cgattcggga tagcggtaat ttctgggtgt ggacagtcac gtgggccgat ctcaaagagc
    20161 ctgggcttaa acatgttcag gatgtcctgg gacttgggca taacccggat atgaaacagc
    20221 ctaagtttta taacctgttt catgatacca actttgcggc ccttgaagcg tcgttccggg
    20281 aacgcaacag ttttgcactg ctgctggatt atctcgccga tccgggcgga agaacgcagt
    20341 tatggcagag aatggctgct gcacatgcct gggtgtggtt ggatgtgaag aagtcacagg
    20401 acgcgggaac gaaacaaaag tatgcctatg aaatgcagga aaacgcgcca gcctggcggc
    20461 tagcagagct tttgccagac gaaccgtttg tctttggtgg tttactcgat agctgtaact
    20521 catcgcagca atttattgag ctggcttcgg tgttgccaca acaagccatt aaaccgacga
    20581 ccagcgtcgc acaaatgcgc agctggctgc gtttgcatat ctgttttgac gatcgctata
    20641 cgcaggatga cggttatgaa gcgggactca atggcttctg gcgactggta aacctgttac
    20701 agttcctgcc ggatatgacc tttaccagcc gtaaagccgt gcatttaccg caggaaagcg
    20761 ttgacactaa agtttcggca acgccagagg cagtaccaga gactgatgag agttgggctg
    20821 agattattga gtttggctta ttaagcgctg aagagatcgc gttgcttcag tctcattctc
    20881 tctctgcgcc gactctcggc tacgaattgc aaaatgatga aggtgaaatt attgcggaag
    20941 ccgatctcgc ctggccatca caaaaatggg cattgattat tgataatcaa gaatttattc
    21001 cattattcac aaccaggggt tggcacgttg cattcgggcc aattgacgaa aatacattgc
    21061 aacatctgtc cggaggtgac aaatgacggt gcaaacgccc aaggtggcac tttctgatgg
    21121 ttttctgggc gcattcgcgc ggatcccaaa ggcacagcaa aaaaaggtgc aggaattcat
    21181 ctccaaattc cgtcaggacc cgaccagtaa tggacttaac tatgaaaaga tccacgatgc
    21241 gcgcagtaaa aatgtccatt cggtgcgaat cgatcaaact tatcgcggca ttgttctgaa
    21301 acctgaacag ggtgccctgt atatgctgat gtgggtggat aaacacgacg aagcatacga
    21361 ctgggcgcgg cggcatgatt gttcgattca cccggttacc ggcgcgattc aggtcattga
    21421 tattagctat atcaaaccgg caccggaagc ggtggttgat aaaccgaaac tcttcgcggc
    21481 ttacagcgcg gaacagattc tggccctggg cgtgccgcct gtctttattg atcaggtgat
    21541 ggcgcttacc gatgaagccg ggcttaatca acttgaaagc ataatgccag cagaagcctg
    21601 ggaacctctg cactggctgg cagaagggct ggattaccag gaagtgctgg aagagtttaa
    21661 cgatcatcat gatgagcctg ttgataccaa cgactttatg gaggcgattg aacgaagtaa
    21721 acgtcgcttc catgtcgttg aaaatgaaca ggagctgttg cagatgctta atgcgccttt
    21781 agaaaaatgg cgtgtatttc tgcatccgag ccagcgaaaa ctggtggagt cgcccgctaa
    21841 tggtccggta cgagtactgg gcggtgcagg tacagggaaa acggttgttg cgatgcaccg
    21901 tgcgcggtgg ttatctcagc gtctggcaga taaacccggt aagaaggtac ttttcacgac
    21961 ctttacgcgt aacctggcgg cagatattcg cgctaatctc cagcggttgt gtacgcgaga
    22021 agagatggcg cgtattgacg tcgttaacat tgatgcctgg atgagcgacc aactaaaacg
    22081 ccacggttat gatttccgcg tggtatacga cagcgatgaa gggcgacgca aatgctggaa
    22141 ctatgccttg cagcaggcac ctgccgaact ggggctacct gacaatttct atgctgaaga
    22201 gtggcaacgc gttgtccagc caaatgcggt ttacacccgg gaggaatacc tcaaagtcag
    22261 ccgggttgga cgtggtacag cgttatcccg tattcaacgg gcgaaaatct ggccagtctt
    22321 tgaagaattc agagcacaaa tggcgcgtgc caaactgcgc gaaatgggcg atgccatgca
    22381 tgaggctatt gttctgttta aagaaaaaca ggttcaacta ccttatagca gtattgtggt
    22441 cgatgaagct caagatatcg gtgccccggc atttactctt attcgcagcc tggttcccga
    22501 gtcgcccaac gatctgttta ttgtcgggga tgggcatcag cgtatttatc gcaataaggt
    22561 cgttcttggt cagtgtggta ttaacgtccg tggtcgtcgc agtaaaaaat tgaagattaa
    22621 ctatcggacc acagaagaga cgcgtcaatt tgctgttggg ctgttaactg gtgtgaaggt
    22681 tgataacctc gatggcgaag cggataccag taatgattat ttgtcattac tgcacgggga
    22741 aaagcccatg attacgcatg cggcagattt caaagaagag gctgcaacgc tcgtcaagca
    22801 aattcaggca ttgctggcga atcaggttcg ttctcaggat atctgcatta ctgcgcgtac
    22861 taaacatgct tgtgaccgct acgcatcagc actgaatgac gccggaattg agacatttaa
    22921 tttaggcaat gatagcggtg acagcgatgc acgccctggc gtgcgggttg caacgatgca
    22981 ccgtattaaa gggcttgagt tccagtatgt gttcctggtg gggattaacg aaggcgttgt
    23041 gccggaaata aaagcattag catccgatga tcccgttgaa cagcgtgatg cattatttaa
    23101 tgagcgcgcg ttactgcatg tggctgcaac ccgtgccgta aaagggctgt ttgtttcatc
    23161 atctggtgtc cctagtccgc tattagtggc tgactgataa tatgtccaaa acagaccagg
    23221 agccgaaacg ctcctggtct atataaccgt tctggcaact ggcgatattt atattgctta
    23281 gctcccaaca gccagacaat atcaacttag aaattatatt cgcttatgcg gtttgcatat
    23341 tgcacagtac aacctgtttt ctctatatct aacgtaacgt caggatgtgg atgatttttg
    23401 gatggggtga cacactggac aaatatagct ggcacgaaaa ggctggcatt tccagctga
//
LOCUS       DAZUGK010000058        23349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZUGK010000058 DAZUGK010000000
VERSION     DAZUGK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.058"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..3185
                     /gene="yfaL"
                     /locus_tag="ACHY5W_001978"
     CDS             <1..3185
                     /gene="yfaL"
                     /locus_tag="ACHY5W_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFS4446880.1"
                     /translation="NNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAFTNN
                     TAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSSAAGGFMY
                     LGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSGDLVLNADNNDFTGEMQ
                     IENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQTFVHS
                     LTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLAGAQSMALTGDIVV
                     DAGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQSGTSYNDGLAVSGSGGT
                     VIGSQDVVDLAGGDNLHIGGDGKDGVYVVIDAGDGLVSLANNNSYLGTTQIASGTLMV
                     SDNSQLGDTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHGRDIEMRADGEVAVDAG
                     VDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIL
                     PYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLRLTGQDTSVALNASLFN
                     GDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNGVAGDTLT
                     VNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVNSITGIGEPTSTGIKVV
                     DFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDPDPTP
                     DPDPTPDPDPTPAPEPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLN
                     LRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSR
                     SSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYAWFSNDVSEHEDGVDHY
                     HSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTR
                     LGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQ
                     RVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3326..4048)
                     /gene="ubiG"
                     /locus_tag="ACHY5W_001979"
     CDS             complement(3326..4048)
                     /gene="ubiG"
                     /locus_tag="ACHY5W_001979"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFS4446881.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4195..6822
                     /gene="gyrA"
                     /locus_tag="ACHY5W_001980"
     CDS             4195..6822
                     /gene="gyrA"
                     /locus_tag="ACHY5W_001980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFS4446882.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6971..8659
                     /gene="yfaA"
                     /locus_tag="ACHY5W_001981"
     CDS             6971..8659
                     /gene="yfaA"
                     /locus_tag="ACHY5W_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFS4446883.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPALAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLNKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGNKRWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGATFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRTAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            8656..9279
                     /gene="yfaT"
                     /locus_tag="ACHY5W_001982"
     CDS             8656..9279
                     /gene="yfaT"
                     /locus_tag="ACHY5W_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFS4446884.1"
                     /translation="MRNGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9303..13817
                     /locus_tag="ACHY5W_001983"
                     /pseudo
     CDS             9303..13817
                     /locus_tag="ACHY5W_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            13818..15466
                     /gene="yfaQ"
                     /locus_tag="ACHY5W_001984"
                     /pseudo
     CDS             13818..15466
                     /gene="yfaQ"
                     /locus_tag="ACHY5W_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            15471..16247
                     /gene="yfaP"
                     /locus_tag="ACHY5W_001985"
     CDS             15471..16247
                     /gene="yfaP"
                     /locus_tag="ACHY5W_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFS4446885.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            16522..19371
                     /gene="rcsC"
                     /locus_tag="ACHY5W_001986"
     CDS             16522..19371
                     /gene="rcsC"
                     /locus_tag="ACHY5W_001986"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFS4446886.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSCKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTGKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(19571..20221)
                     /gene="rcsB"
                     /locus_tag="ACHY5W_001987"
     CDS             complement(19571..20221)
                     /gene="rcsB"
                     /locus_tag="ACHY5W_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFS4446887.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20238..22910)
                     /gene="rcsD"
                     /locus_tag="ACHY5W_001988"
     CDS             complement(20238..22910)
                     /gene="rcsD"
                     /locus_tag="ACHY5W_001988"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFS4446888.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5657 a         5733 c         6412 g         5547 t
ORIGIN      
        1 acaataacca ggcatataca tcaacaagtt acagtgatgg cgatggcggc gcaatcgatg
       61 tcacagataa taatagcgac agcaagcatc cttcaggtta tacgataata aataacactg
      121 cctttacaaa taacactgcc gaaggttatg gcggggcgat atataccaat agcgcgacgg
      181 ctccctatct tattgatatt tctgttgatg acagctacag ccagaacgga ggcgtgttag
      241 tcgatgagaa caatagcgca gcaggctatg gagatggtcc ttcctctgcg gcgggtggct
      301 ttatgtatct cggcttaagt gaagttacct ttgatattgc cgacggaaaa acgctggtta
      361 ttggcaatac agagaatgac ggagctgttg actctattgc tggtaccggg gtaatcacca
      421 aaacaggttc cggcgatctg gtacttaatg cagataacaa tgactttact ggcgagatgc
      481 agatagaaaa cggtgaagtt accctgggcc gcagcaactc cctgatgaat gtcggcgata
      541 cgcattgcca ggacgatccg caagactgct acggtctgac gatagggagt attgataagt
      601 accagaatca ggcagagcta aatgttggct ccacccaaca aacctttgtg cactcattga
      661 cgggctttca gaatggcact ttaaatatcg atgctggtgg caatgttact gttaatcaag
      721 gcagttttgc tggcaccatc gaaggtgctg gtcagctcac cattgcgcaa aacggcagct
      781 atgtgctggc gggggcgcag tcgatggcgc taaccggcga tatagtggtg gatgctggtg
      841 cggtgctttc gctggaaggc gacgcggcag atcttgccgc tctccaggac gatccgcagt
      901 cgatcgtgtt aaacggcggc gtgctcgatc tctctgattt cgccacctgg cagagcggca
      961 catcatacaa cgatggcctt gcagtcagcg gcagcggcgg tacagttatc ggcagtcagg
     1021 atgtggtgga tctcgcaggt ggcgacaatt tgcatatcgg cggcgacggg aaagatggcg
     1081 tctacgtggt gatcgatgcg ggcgacgggc tggttagcct ggcaaacaat aatagttacc
     1141 tgggcaccac gcaaatcgct tctggtacgc tgatggtgag cgacaactcg cagcttggag
     1201 atacccatta caaccgccag gttatcttta ccgataagcc acaagaaagc gtgatggaga
     1261 ttactgccaa tgtcgatact cgctctacaa cgactgagca tgggcgtgat attgaaatgc
     1321 gcgccgacgg tgaagtggca gttgatgcgg gggtagacac gcagtggggc gcactgatgg
     1381 ctgacagcag cgggcagcat caggatgagg gtagcacatt gacgaaaacg ggggcgggta
     1441 cgctggagct gaccgccagc ggtacaacgc agtcggcggt acgtgtcgaa gaaggcaccc
     1501 tgaaaggtga tgttgctgat atccttcctt atgcttcgtc actgtgggtt ggtgatgggg
     1561 caacgttcgt tactggcgcg gatcaggata ttcagtcaat tgatgctatt tccagcggca
     1621 ctatcgacat cagcgatggt acggttttgc gcctgaccgg gcaggatact tccgtcgccc
     1681 ttaatgcctc actatttaac ggcgatggga cgctggtgaa tgccaccgat ggtgtgacgt
     1741 tgacaggtga gcttaatacc aaccttgaaa ctgacagcct gacttatctt tccaacgtga
     1801 cggttaatgg caatctgacc aatacgtccg gtgcggttag cctgcaaaat ggcgtcgctg
     1861 gcgatacgct gacggtaaac ggtgattaca ccggcggcgg tacgttactg ttcgacagcg
     1921 aattaaacgg cgatgactca gcaagcgacc agttggtgtt gaacggtaat actgctggca
     1981 acacaactgt ggtggttaac tccattacag ggattggtga gccgacatcg acaggcatta
     2041 aagtggttga tttcgcagct gatcccacgc agtttcaaaa caatgcgcag ttcagtctgg
     2101 caggcagcgg ctacgtcaat atgggagcgt atgactacac gctggtggaa gataacaacg
     2161 actggtatct gcgatcgcaa gaagtaacgc cgccatcgcc acctgatcca gacccgactc
     2221 ccgatcctga tcccacgccg gatcctgatc caacacccgc cccggaacct acgcctgctt
     2281 accagccggt gttgaatgcc aaagttggcg gttatctcaa taacctgcgg gcggcaaatc
     2341 aggcgtttat gatggagcga cgcgatcacg caggtggcga tggtcagacg ctgaatttac
     2401 gtgttatcgg cggagattat cattacacag cagcggggca actggctcaa catgaagaca
     2461 cttctacggt gcaacttagc ggcgatctgt ttagcgggcg ctggggcacg gatggcgagt
     2521 ggatgcttgg gattgttggt ggctacagcg ataaccaggg cgacagccgc tcgagtatga
     2581 ccggaactcg cgccgataac cagaaccacg gttatgcggt tgggctgacc tcaagctggt
     2641 ttcagcacgg taagcagaag cagggggcct ggctggataa ctggttgcag tacgcgtggt
     2701 ttagcaatga tgtttctgaa catgaagatg gcgtggatca ttaccattcg tcggggatta
     2761 tcgcctcgct ggaagcgggg tatcagtggt taccggggcg tggtgtggtg attgaaccgc
     2821 aggcgcaggt gatttatcag ggcgtgcagc aggatgattt taccgccgct aaccgtgcgc
     2881 gcgtgtcaca atcgcagggt gatgatattc agacgcggct gggtttacac agcgaatggc
     2941 gtaccgctgt tcatgtcata ccaacattag atctgaatta ttatcacgat ccccattcga
     3001 cggaaattga agaggatggc agcactatca gtgacgatgc ggtgaagcaa cggggggaaa
     3061 taaaagtggg agtcacgggc aatatcagtc agcgagtgtc cctgcgcggc agcgtggcgt
     3121 ggcagaaagg gagtgatgat tttgcccaga cggcagggtt tttgtcgatg acggtgaaat
     3181 ggtaatcgct gtctgaccat cactattgcc ggatgcggcg taaacgcctt atctggccta
     3241 cggagagcat ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca ttggcgcggt
     3301 tgccggatga atccaaacaa ccatctcact tattctgcgt gtgcagcata tagttcacat
     3361 ccacgccggg gccgagctta aaggagttag tgatcgggtt gtaatgcagc ccggtcatat
     3421 gacgttcttt aagactggtc tgatccaccc agcccagcag ttctgccggt tttataaatt
     3481 tcttcacgtc gtgcgtacct ttaggcacca tgcgcaaaat atattcagcg ccaaccaccg
     3541 ccatcagcca ggacttgccg ttgcggttaa gcgtcgagaa aaagacatcg ccgcccggtt
     3601 tcaccagttg cgcgcaggct ctgaccactg actgcggatc gggaacgtgc tccagcatct
     3661 ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct tccacagttt
     3721 cttgcacgta atccacctga atgccgcttt ccagcgcgtg cagttttgcc acctgcaatg
     3781 gctcaaagcc catatccaga ccggtcaccg tcgcaccttc gcgcgccata ctctcggcca
     3841 gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataagccg ccagcacgct
     3901 cggcaatata gcccagacgc agcgggttaa tgcggtgcag cggtttgaac tcaccttcca
     3961 gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg tctacgttat
     4021 ggtttaccgg cgatttttcg gcattcattg gcacttctac tccgtaattg gcaagacaaa
     4081 cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa ctgcgcggct
     4141 gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg ttagatgagc
     4201 gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag ctcatatctg
     4261 gattatgcga tgtcggtcat tgttggccgt gcgctgccag atgtccgaga tggcctgaag
     4321 ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg gaacaaagcc
     4381 tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc ccatggtgac
     4441 tcggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg ttacatgctg
     4501 gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc aatgcgttat
     4561 acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga aaaagagacg
     4621 gtcgatttcg ttgataacta tgacggcacg gaaaaaattc ccgacgtcat gccaaccaaa
     4681 attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc aaccaacatc
     4741 ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga tgatgaagac
     4801 atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac ggcggcaatc
     4861 attaacggtc gtcgcggtat tgaggaagct taccgtaccg gtcgcggcaa ggtatatatc
     4921 cgcgcccgtg ccgaagtgga agttgacgcc aaaaccggac gtgaaaccat tatcgtccac
     4981 gaaattccgt atcaggtgaa caaagcgcgc ctgatcgaga agattgcgga actggtaaaa
     5041 gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga cggtatgcgc
     5101 atcgtgattg aagtgaaacg cgatgcggtc ggtgaagttg tgctcaacaa cctctactcc
     5161 cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca tggtcagccg
     5221 aagatcatga acctgaaaga tatcatcgcg gcgtttgttc gtcaccgccg tgaagtggtg
     5281 acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat ccttgaagca
     5341 ttagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca tgcgccgacg
     5401 cctgcagaag cgaaaactgc gctggttgct aatccgtggc agctgggcaa cgttgccgcg
     5461 atgctcgaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc agagttcggc
     5521 gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct ggatctgcgt
     5581 ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa agagctgctg
     5641 gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtctgat ggaagtgatc
     5701 cgtgaagagt tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac tgaaatcacc
     5761 gccaacagcg cagacatcaa cctggaagat ctgatcaccc aggaagatgt ggtcgtgacg
     5821 ctctctcacc agggctacgt taaatatcag ccgctttctg aatacgaagc gcagcgtcgt
     5881 ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga ccgactgctg
     5941 gtggcgaaca ctcacgatca tatcctgtgc ttctccagcc gtggtcgcgt ctattcgatg
     6001 aaggtttatc agttgccgga agccactcgt ggcgcgcgcg gtcgtccgat cgtcaacctg
     6061 ctgccgctgg agcaggacga acgtatcacc gcgatcctgc cggtgaccga gtttgaagaa
     6121 ggcgtgaaag tcttcatggc gaccgctaac ggtactgtga agaaaaccgt cctcaccgaa
     6181 ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgacgg cgatgagctg
     6241 atcggcgttg atctgaccag tggcgaagat gaagtaatgc tgttctccgc cgaaggtaaa
     6301 gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaataccac tggtgtgcgc
     6361 ggtattcgct taggcgaagg cgataaagtc gtctctctga tcgtgcctcg tggcgatggc
     6421 gcaatcctca ccgcaacgca aaacggttat ggtaagcgta ccgcagtggc ggaataccca
     6481 accaagtcgc gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg taacggttta
     6541 gttgttggcg cggtacaggt agatgactgt gaccagatca tgatgatcac cgatgccggt
     6601 acgctggtac gtactcgcgt ttcggaaatc agcatcgtgg gccgtaacac ccagggcgtg
     6661 atcctcatcc gtactgcgga agatgaaaac gtggtgggtc tgcaacgtgt tgctgaaccg
     6721 gttgacgagg aagatctgga taccatcgac ggcagtgccg cggaagggga cgatgaaatc
     6781 gctccggaag tggacgttga cgacgagcca gaagaagaat aatttcatcg cttcatgcca
     6841 aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc tgggcttttg
     6901 tcgtattagg gcgcggtaaa gtttggctgt gcccgtaaaa aatggctggc tatacacaag
     6961 gaatgtggca atgagtggtg aaaaaaaggc gaaaggctgg cggttctatg gtcttgtagg
     7021 ttttggcgca atagcactgc tttccgctgg tgtctgggcg ttgcaatatg ctggcagtgg
     7081 gccagaaaaa acgttgtcgc cgttggtggt gcacaacaat ctgcaaatcg atctcaatga
     7141 gccggacctc tttctcgaca gcgactctct gagccagctt cccaaagatc tccttaccat
     7201 tccgtttctc cacgatgttc ttagcgaaga tttcgttttc tattatcaga atcatgccga
     7261 tcgtctgggc attgaaggca gcattcgccg cattgtctat gaacacgatc tcacgctgaa
     7321 agataagctc ttttcgtcac tcttagatca gcccgcgctg gcggcgctgt ggcacgataa
     7381 acaaggccat ctttcacatt acatggtgct gatccagcgc agtggtttaa gcaaactgct
     7441 ggagccattg ttgtttgccg ctaccagcga cagccagtta aacaaaacgg aaatcagtag
     7501 catcaagata aatagtgaaa ctattcctgt ttatcagttg cgctataacg gcaataacgc
     7561 cctgatgttc gcgacatatc aggacaagat gttggtgttt tccagcacgg atatgctgtt
     7621 taaagatgac cagcaggata cggaagccac ggcgatcgca agtgatttgt tgagcggcaa
     7681 taaacgctgg caagccagct ttggcttgga agagcgtgct gccgaaaaaa cgccagtacg
     7741 ccagcgcatc gtggccagcg ccaggttgct gggttttggc taccagcggt taatgccttc
     7801 ttttgctggc gtacgcttcg aaatgggtaa cgacggctgg catagctttc tggcgttaaa
     7861 tgatgaatcc gccagcgtag atgccagttt cgattttacg ccagtctgga acagtatgcc
     7921 tgccggggcc accttctgtg tggcggtgcc gtattcacac ggtattgccg aagagatgct
     7981 ttcgcacatc agccaggaaa acgacaagct gaatggggcg ttagacggtg ccgcggggct
     8041 gtgctggtat gcagactcaa aattgcaaac cccgctgttt gtcggtcagt ttgatggcac
     8101 tgccgaacag gcgcaattgc cagggaaact gtttacgcaa aatattggtg cgcacgaaag
     8161 caaagcgcca gaaggtgttt tgccggtaag ccagactcag cagggcgaag cgcaaatctg
     8221 gcgtcgcgaa gtgagttccc gatacggtca gtatccgaaa gcgcaggcgg cgcaacccga
     8281 tcaattaatg tcggattatt ttttccgcgt gtcgctggcg atgcaaaaca aaacgctgct
     8341 tttctccctc gatgacacgc tggttaataa cgccctgcaa acactgaata aaacccgcac
     8401 ggcaatggtg gatgtaatac ccactgatgg catcgttccg ctctatatca atccacaagg
     8461 cgtggcgaaa ctgctgcgta acgaaacgtt gaccagtctg ccgaagaatc tcgaaccggt
     8521 tttttataac gccgcacaaa ctttattaat gccgaagctg gacgctttat ctcaacaacc
     8581 gcgttatgtc atgaagctgg cccagatgga acccggtgcc gcctggcagt ggctacccat
     8641 aacctggcag ccgctatgag gaacgggctg ctggcgctga tttgctggct gtgttgtgtt
     8701 gttgcccata gcgaaatgct gaatgtcgaa caatccgggc tgtttcgcgc ctggtttgta
     8761 cgcattgcac aagaacagct ccgccagggg ccaagtccac gttggtatca gcaggattgt
     8821 gcgggcctgg tgcgatttgc ggcgaacgag acgctgaaag ttcacgacag taaatggcta
     8881 aaaagtaacg gtttatctag ccagtatttg ccgccagaga tgacgctaac acctgaacag
     8941 cggcaactgg cgcaaaactg gaatcagggg aacgggaaaa ccggccccta cgtaaccgcg
     9001 attaatttga ttcagtacaa cagccaattt attggccagg acataaacca ggcgctgcct
     9061 ggcgatatga tttttttcga tcagggcgat gcccagcact taatggtctg gatggggcgt
     9121 tacgtcatct accacaccgg aagcgccacg aaaactgaca acggaatgcg cgcagtcagt
     9181 ctgcaacaac ttatgacatg gaaggacacc cgatggatac ccaacgattc caatcccaat
     9241 ttcattggca tttatcgttt aaattttctg gcgcgatagc agcgtgcttg tgcctgtctc
     9301 ttgtgggaac agagcttgct aatgctgatg attcgcttcc ttccagtaac tatgcgccgc
     9361 ccgccggggg aacattcttt ttgcttgctg acagcagttt tagcagcagt gaagaggcga
     9421 aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa gagtacggcg
     9481 gcgtggacgt tcgcctgtat cgtattcctg acccgatggc atttttgcgc cagcagaaaa
     9541 acctgcatcg cattgtggtg caaccgcaat atttaggcga cgggctgaac aatacgctga
     9601 cctggctgtg ggataactgg tacggcaaat ctcgccgcgt gatgcagcgt actttctctt
     9661 ctcagtcacg gcagaatgtg actcaggcat tacccgaatt acatctcggc aatgccatta
     9721 ttaaaccttc ccgttatgta cagaacagcc agttttcgcc gctgaaaaaa tatcccctgg
     9781 tggaacagtt ccgttatcca ctatggcagg ctaaaccggt cgagccgcag caaggggtaa
     9841 aactggaagg cgcatccagc aatttcatct cgccgcagcc gggtaacatt tatattcctc
     9901 tcggccaaca agagccggga ctgtacctcg tcgaggcgat ggttggtggg tatcgggcga
     9961 cgacggtggt gtttgtttcc gataccgtgg cgcttagcaa agtgtcaggc aaagagcttc
    10021 tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag atcttgtgga
    10081 ctgacggtct tggcgtgatg acccgcggtg tgaccgatga cagcggttcc ttgcagttac
    10141 aacatatatc gccagaacgt tcatacattc tgggtaagga tgctgaaggc ggcgtttttg
    10201 tctccgagaa cttcttctac gaaagcgaaa tctacaacac ccgcttgtat atctttaccg
    10261 atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc cgcgagttcc
    10321 acgatccgtt gcattcatcc cccatcgtca gcgccccggc gaagctttcg gtgctggacg
    10381 ctaacggcag tctgttgcaa accgtcgatg tcacgctgga tgcgcgcaat ggcgggcagg
    10441 ggagtttccg cctgccagaa aatgccgttg ccggaggtta tgagttacgt cttgcttacc
    10501 gcaatcaggt ctatagcagt agttttcgcg tagccaacta tatcaagcca catttcgaga
    10561 ttggtttagc cctcgacaaa aaatagttca gaactggcga agcggtcagc ggcaaactgc
    10621 aactgctcta cccggatggt gagccggtaa aagatgcccg cgtgcagtta agtttgcgcg
    10681 ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc cggacgtttc cccgtgtcgc
    10741 tggaaggcag tgaaacggtg tccgacgcca gcggtcatgt ggcgttaaat ctccccgtcg
    10801 ccgataaacc gagccgctat ttgttaaccg tctccgccag tgacggcgcg gcgtatcgcg
    10861 tcaccaccac caaagagatc ctcattgaac gcggcctggc gcattactct ttaagtaccg
    10921 ccgcacaata cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt
    10981 caaaacaggt tcctgttacg tatgaatggt tgcgtctcga agaccgcacg agccatagcg
    11041 gagagctacc gtcaggcggc aaatccttta ccgtcaattt cgataaacct ggcaactaca
    11101 atctgacatt acgcgataaa gacggcttaa ttctcgctgg gttaagtcat gccgtcagcg
    11161 gtaagggcag cacggcgcat actggtacgg tagatatcgt ggcggataaa acgctgtacc
    11221 agccaggcga aaccgcgaag atgctgatta cctttccgga gccaattgat gaagcattat
    11281 tgacgctgga acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg gcaaactggc
    11341 taacgctaca acgtttaaac gatacccagt atgaagcccg ggttccagtg agcaattcct
    11401 ttgcgcctaa catcactttt tcggtgctgt atacccgtaa tggtcagtac agttttcaga
    11461 acgccgggat caaagttgcc gttcctcagc tggatatccg ggtgaaaacg gacaaaaccc
    11521 attaccagcc tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg
    11581 tttctgcgca gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg caaccagaaa
    11641 tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg taacaatgtg cgtaccagct
    11701 ccagtctgtc gtttatcagc tacgaccagg cgctctccag cgagccggtt gcgcctggcg
    11761 caactaaccg cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt gaagaggtgg
    11821 ataccgcggc atggatgccg tcactcacaa ccgataaaca aggcaaagcg tatttcacgt
    11881 tcctgatgcc tgattcgtta acccgctggc gtatcaccgc gcgtgggatg aacggcgacg
    11941 ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa aaatctctac atgaagtgga
    12001 gtatgccaac ggtgtatcgc atgggcgaca aaccggcagc aggactgttt atcttcagtc
    12061 agcaggataa cgaaccggtg gcgctggtga ctaaatttgc aggcgctgag atgcgccaga
    12121 cgctgacgct gcacaaaggg gcgaattata tttcgctgac gcagaacatt cagcaatctg
    12181 gcttgttaag cgcagaactg caacaaaatg ggcaagtgca ggacagcatt agcacaaaac
    12241 tgtcttttgt ggataacagc tggcccgttg aacagcagaa aaatgtcatg ctcggcggtg
    12301 gcgagaacgc gctgatgttg cccgagcagg ctagcaatat ccggctacaa agtagcgaaa
    12361 cgccgcagga gattttccgc aacaatcttg atgggttagt cgatgaacca tggggtgggg
    12421 tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg cttgccgatc
    12481 atcaaagtgc caccgctaac gacattcgtc agatgattca ggataaccgt ctgcggctga
    12541 tgcaactggc ggggcccgga gcgcgcttta cctggtgggg tgaagatggc aatggtgacg
    12601 ccttccttac ggcatgggca tggtacgccg actggcaggc cagccaggcg ctcggcgtaa
    12661 cgcaacaacc ggaatactgg cagcatatgc tcgacagtta cgcggagcag gcggacaaca
    12721 tgccgttatt gcatcgggcg ttggtactgg cgtgggcgca ggagatgaat ctgccgtgca
    12781 aaacattgct aaaagggttg gatgaagcta tcgcccggcg cggaactaaa actgaagatt
    12841 tctctgagga agacaccagc gatatcaatg acagcatgat cctcgataca ccggaatctc
    12901 cactggcaga tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga
    12961 agtccacggt gatgccacag gttcagcaat atgcgtggga taaagcggca aacagcaatc
    13021 agccgctggc acacacggtt gtgctgctca atagcggggg cgacgctacc caggcggctg
    13081 ctattttaag tggtttgacc gctgagcaat ctactattga gcgcgcgctg gccatgaact
    13141 ggctggcgaa atatatggcg acaatgcctt cggttgtgtt gcctgcgcct gcgggcgcat
    13201 gggccaaaca taagttaact ggagggggcg aatactggcg ttgggttggt cagggcgtgc
    13261 cggacattct ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg
    13321 aaccggcaaa aacggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc
    13381 ggcttatccc tggtgaagaa gagatgagct ttactctgca gccagtgacc agcaatgaga
    13441 ttgacagcga tgcgctgtat ctcgatgaaa tcacgcttac taacgagcag gatgcagttc
    13501 tgcgctacgg tcaggtggaa gttccgctcc cgccgggagc cgacgttgaa cggacaacgt
    13561 ggggcatttc ggtcaataaa cctaacgccg cgaaacagca ggggcaattg ctggaaaaag
    13621 cgcgtaatga aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg
    13681 tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc
    13741 gttatgtgcg ttcctatgca cctgcgcagc aaagtgttgc ggcagggagt gaatggaccg
    13801 ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gatattggcg ttggttactc
    13861 tgccaacgct tgcggaggag acgccgttac aactggcact gcgtggtgcg cagcacgatc
    13921 aactgtatca gctgtcgtca tcaggcgtga ccaaagtctc agcattacct gactcgctca
    13981 ccacgccgtt agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc
    14041 aaccggagca gccctaccag tgtcgcggaa actccccgga agaggtttat tgttgtcagg
    14101 ctggggagag cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc
    14161 cgcagcggtt gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcac
    14221 ctgcgtggtt agcgtctctt tcttcgctga agccggaaac ctcggtaacg gttaaatcgc
    14281 tgcttgattc attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg
    14341 tggtgctgga cgaggcgaaa atcggcgtcg catctatgct cggtagtcgg gtacgggtga
    14401 aaacctggag ctggtttgcc gataataaac aggaaatccg tcagggtggt tttgctggct
    14461 ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa acggtactga
    14521 cccgttatgc gacggtgctt aatcgggttt tgccagtgcc gacacaagtt gccagtgggc
    14581 aatgtgtaga ggtggagcta tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga
    14641 gtacgacggc gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacat ttgccaatgg
    14701 taaccacatt acgtttgtca gccacggtga gacgacctta ctgaccgaaa agggcaaact
    14761 gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
    14821 gaaaagtacg ccacctgagg cagcgaaagc catgaccgtg gcgatacgca cttttttaca
    14881 gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg ccacgcagcg
    14941 tgtttctgct tcgccagcga ccacgggggc gcgaacgatg accgcctgga cgcaggatct
    15001 catctatgcg ggcgatccgg ttcattatca cggcagtcgc gctaccgaag ggacgctttc
    15061 ctggcggcaa gccatggccc aggcggggca gggagagcgt tacgaccaga tactcgcgtt
    15121 tgcttatccc gacaacagcc ttagccgctg gggagcgcca cgctcaacct gtcagttatt
    15181 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
    15241 cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
    15301 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
    15361 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
    15421 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
    15481 tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg
    15541 aagtcgagat tgacgcaccg ctctcaggct ggcatccggc agagggcgaa gatgccagtt
    15601 tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
    15661 cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggcc
    15721 ggctggtggt caacggtgcc agtatgccgc agagaattga atccgatggt tcatttgcac
    15781 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa
    15841 gccgacaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt gcacgtttac
    15901 ggctggttct ctcgtgggat acggacaata ccgatctcga tctccatgtt gtcacgcccg
    15961 atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg
    16021 atgtcacgac ggggtatgga cccgagattt tcgccatgcc agcgccagtt cacggacgct
    16081 atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattaacc accgcccaac
    16141 tgacgctgat caccgatgaa ggatcggtca atgagaaaca ggaaacattt attgtaccga
    16201 tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatcc atcccttcac
    16261 acaacctggc tctttctttt ttcagataag actgctgggc gatctgccga ccccaggata
    16321 taaccattaa caagcgtcgg cagattggtc atttcttatt tttcgtactg agaactgtcc
    16381 atttactccg gctttcctgg ttaaggcaat ggtttctaag cgatacgcat tattgcaatg
    16441 gtgacagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
    16501 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc
    16561 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
    16621 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
    16681 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
    16741 aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
    16801 gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg ccgactccga ttgttccgca
    16861 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
    16921 gataattttt ctgcggctta cgatctcaac cgggtatttt taatcggcag cgataacctc
    16981 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagcg
    17041 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
    17101 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
    17161 tatctggcga acaggttgca ggcgcttttg ggtgtcgagc agaccatccg gatggagaat
    17221 tttttcttac cgggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
    17281 ctgatttcgc ttaccggacc agaaagcaaa attaagggcg atcctcgctg gatgcaggaa
    17341 cgctcctggt ttggctatac ggaagggttc cgggagctgg tgctgaagaa aaatctgcca
    17401 ccctcatcgc taagcatcgt gtattcggtg ccggttgata aggtgctgga acgcattcgc
    17461 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
    17521 gcgcggatgt acgagcgacg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
    17581 gaacatgagc agtttaatcg caagattgtc gcctccgcgc cagtgggtat ctgcattttg
    17641 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
    17701 cttacgcatg aggaccgcca acgactgacg caaattatct gtgggcagca ggtcaatttt
    17761 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
    17821 cgtaatgaaa acgtggccat ttgtgtactg gtggatgttt cttcgcgcgt gaagatggaa
    17881 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
    17941 cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
    18001 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcggc tggtgacggc aatgaacaac
    18061 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa gattgaatcg
    18121 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
    18181 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
    18241 gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
    18301 ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt ccgcgctgat
    18361 ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc gaaagaagtg
    18421 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa cttccagggg
    18481 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg
    18541 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
    18601 cagtacccgc agaaaaaagg cgtggaaggg ttgagttgta aacgctgctg gctggcggtc
    18661 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgta
    18721 acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga cgaggtagtg
    18781 agtaaaaaat ggcagggcag agcggtagtg accttctgtc gtcgacatat tggtattccg
    18841 ctggagaaag cgccagggga gtgggtacac agtgtggctg ctccgcatga gctaccggca
    18901 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
    18961 tcgacgggca aagcggtcag cgataatgac gatatgatga ttctggtcgt ggatgatcat
    19021 ccgattaacc gacgtctgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
    19081 gcgaatgatg gcgtcgatgc gcttaatgta cttagcaaga atcatattga tatcgtgctt
    19141 agcgacgtca acatgcccaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    19201 ggactgacgt tgccggtaat cggagtaact gctaatgcgt tggctgaaga gaagcagcgg
    19261 tgtctggagt ccggtatgga cagctgcctg tcgaagccag taacgctgga tgttataaaa
    19321 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggccggataa
    19381 ggcgtttacg ccgcatctgg cattcagtgc aaatgccaga tgcggcgctg acgcgtctta
    19441 tctggcctac tttaatgtaa tctgttgaaa taatgggaat cgtaggacgg ataaggcgtt
    19501 tacgccgcat ccggcaatcg gtgcaaatgc cagataagac actaacgcgt cttatctggc
    19561 ctacaggtga ttagtcttta tctgccggac ttaacgtcac tgaagagaga taattcagca
    19621 gggcgatatc gttctcgaca cccagcttca tcatcgcaga tttcttctgg ctactgatgg
    19681 ttttaatact gcggttcagc tttttagcga tctcggtcac caggaagcct tccgcaaaca
    19741 ggcgcagaac ttcactctct tttggcgaga gacgtttgtc accgtaacca ccagcactga
    19801 ttttttccaa caggcgagaa acgctttccg gggtaaattt cttccctttc tgcagtgcgg
    19861 cgagagcttt cggcagatcg gtcggtgcac cttgtttcag cacgatccct tcgatatcca
    19921 gatccaatac cgcactaaga atcgccgggt tgttgttcat agtcagaaca atgatcgaca
    19981 ggcttgggaa atggcgcttg atgtacttga ttaaggtaat gccatcgccg tacttatcgc
    20041 caggcatgga gagatcggta atcaacacat gcgcatccag tttcggcagg ttgttgatca
    20101 gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt
    20161 tgcgaatacc gaacaagact atcggatggt catcggcaat aattacgttc atattgttca
    20221 tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc gctgatgtat
    20281 ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc acataactgc
    20341 ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc cgtttgggct
    20401 aacgcagcaa agtcactggt tgctgcttca gtatacagcc tcttaacatc atccggtact
    20461 gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc atctccgccc
    20521 agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg taagaccgct
    20581 tcctgcatag cgttgctcat attgaagttg acgcacaatt gaccaggccc aatttcccgt
    20641 acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga cggattatcc
    20701 gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat acaggttgca
    20761 ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt aacatccacc
    20821 attacgcaga catcatccag taaacgctct tcttcctctt caacttccgg gtcagctgcg
    20881 agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt gatgtttaaa
    20941 tgaccgccca gtttacgtgc cagttgatcg ctcagccaga atgccagcgg gtccgccttg
    21001 ccatagcgat cgttttgggt ctggttgata aacgggaagt gcaaattatc catttcatga
    21061 atgcttacgc cttctccggt gtccagaatg cggaacgtca ggcggtcttc ggaggactca
    21121 tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc atattgcatc
    21181 agtagcagca aaatacgtcg taaggcatcg cgatcgccac ggcgcatatc gtgtgctttc
    21241 agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa cactgaaggc
    21301 acaacttcat caattaaatc ctgcacggag aacagcaccg tctcactttt ccagctatcg
    21361 tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc gcaccagcac atctgcctga
    21421 ttcgccagtt gtttgctttc cggggcgttg agtttagccg cgctctccgc cagggactgt
    21481 gcaggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc ctgctggttt
    21541 ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc gcgatcctga
    21601 tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc tgcggaacat gcggatctca
    21661 tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc catggtggtg
    21721 atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact tatgacagtg
    21781 cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac tatctcttca
    21841 ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg tgttgggtag actttctgta
    21901 ctgcgactgg agaaatggcg gaatgtggta tagccaaata acgccagcgc cagcaaaccg
    21961 atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcaataa ggtgccataa
    22021 ggaacctgcc agaccagtcg catatcggta gagttgagtg ccgaggagat ttcaatcttc
    22081 gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattatc attgtctccc
    22141 gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa
    22201 tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg gttaaaggtg
    22261 gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt agaaaagctt
    22321 tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg cacgacgtga atcaacaatg
    22381 tcgctgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag ggttgagatc
    22441 agcaccagac tgttatcctg accattcagg taatacatcg accacggtac attttctgca
    22501 ccccacaatg tatccagata agtggacatc cgctgagtca tctcaagcgt tgagttgtcg
    22561 tgagagccaa agattaacgc ttccgttttg cggcgtggtt tctccagata gtagacatcc
    22621 tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt cgcggcaatg
    22681 ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg gattgcgtgg
    22741 gtaatgtcga caatctggta acttttatct ttcagccagg cgttaacggc gctttgtacc
    22801 attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg ggtaatgctc
    22861 cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat
    22921 gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt gaaattccag
    22981 actcttacct gattgatgat tctctattct cccgtgatat ttatcatcct gtatgggggg
    23041 aaattgatgt acaagcgcca ttttgctgta caaataacct acgaaaagcc caacgaaatt
    23101 aattgcaaat aaacgaaaat tgctggaaat tatgctgatg ttaattattt gtgaaatagt
    23161 taacaagcgt tatagttttt ctctggtagc acagaataat gaaaagtgtg taaagaaggg
    23221 taaaaaaaac cggatgcgag gcatccggtt gaaatagggg taaacagaca ttcagaaatg
    23281 aatgacggta ataaataaag ttaatgatga tagcgggagt tattctagtt gcgagtgaag
    23341 gttttgttt
//
LOCUS       DAZUGK010000059        23344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZUGK010000059 DAZUGK010000000
VERSION     DAZUGK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.059"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..729
                     /gene="ais"
                     /locus_tag="ACHY5W_001989"
     CDS             127..729
                     /gene="ais"
                     /locus_tag="ACHY5W_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFS4446889.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(768..1193)
                     /gene="nudI"
                     /locus_tag="ACHY5W_001990"
     CDS             complement(768..1193)
                     /gene="nudI"
                     /locus_tag="ACHY5W_001990"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFS4446890.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1472..2014
                     /gene="yfaZ"
                     /locus_tag="ACHY5W_001991"
     CDS             1472..2014
                     /gene="yfaZ"
                     /locus_tag="ACHY5W_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFS4446891.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2114..3316
                     /gene="yfaY"
                     /locus_tag="ACHY5W_001992"
     CDS             2114..3316
                     /gene="yfaY"
                     /locus_tag="ACHY5W_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFS4446892.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTFSV"
     gene            3535..4317
                     /gene="yfaX"
                     /locus_tag="ACHY5W_001993"
     CDS             3535..4317
                     /gene="yfaX"
                     /locus_tag="ACHY5W_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4446893.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4332..5537
                     /gene="rhmD"
                     /locus_tag="ACHY5W_001994"
     CDS             4332..5537
                     /gene="rhmD"
                     /locus_tag="ACHY5W_001994"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFS4446894.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5594..6883
                     /gene="yfaV"
                     /locus_tag="ACHY5W_001995"
     CDS             5594..6883
                     /gene="yfaV"
                     /locus_tag="ACHY5W_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446895.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     IEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWIFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6901..7704
                     /gene="yfaU"
                     /locus_tag="ACHY5W_001996"
     CDS             6901..7704
                     /gene="yfaU"
                     /locus_tag="ACHY5W_001996"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFS4446896.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVQGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7745..7978)
                     /locus_tag="ACHY5W_001997"
                     /pseudo
     CDS             complement(7745..7978)
                     /locus_tag="ACHY5W_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7979..8950)
                     /gene="rpnE"
                     /locus_tag="ACHY5W_001998"
     CDS             complement(7979..8950)
                     /gene="rpnE"
                     /locus_tag="ACHY5W_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFS4446897.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPKTARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(9143..10333)
                     /gene="glpC"
                     /locus_tag="ACHY5W_001999"
     CDS             complement(9143..10333)
                     /gene="glpC"
                     /locus_tag="ACHY5W_001999"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFS4446898.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSWVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10330..11589)
                     /gene="glpB"
                     /locus_tag="ACHY5W_002000"
     CDS             complement(10330..11589)
                     /gene="glpB"
                     /locus_tag="ACHY5W_002000"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFS4446899.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDKTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11579..13200)
                     /gene="glpA"
                     /locus_tag="ACHY5W_002001"
                     /pseudo
     CDS             complement(11579..13200)
                     /gene="glpA"
                     /locus_tag="ACHY5W_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
     gene            13473..14831
                     /gene="glpT"
                     /locus_tag="ACHY5W_002002"
     CDS             13473..14831
                     /gene="glpT"
                     /locus_tag="ACHY5W_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFS4446900.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14836..15915
                     /gene="glpQ"
                     /locus_tag="ACHY5W_002003"
     CDS             14836..15915
                     /gene="glpQ"
                     /locus_tag="ACHY5W_002003"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4446901.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGVMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLHKDN"
     gene            complement(15960..16838)
                     /locus_tag="ACHY5W_002004"
     CDS             complement(15960..16838)
                     /locus_tag="ACHY5W_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4446902.1"
                     /translation="MTLTQIHTLLAVLDYGGFTEASKRLFMTQSAVSQAIATLEQELG
                     VEILIRERRKALQLTDAGSRIASHLRAITREVNAVKEIAEQEKHNPTRTLRIGCFPSA
                     CACILPGIIRYFETYHPQIKIIPYEENSSEIIDSVQNETIDAGFVHFPVTGMYSVPVY
                     QDKFTVVLPDAHPLAKNNAISVEELVGEPLIISKGRYELSIMALFKEKNITPVIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKAVAGELCSVPLEPTFYRQISLLVKEAPVEGSPL
                     FLLQMCMETLVVKGIL"
     gene            17003..18193
                     /locus_tag="ACHY5W_002005"
     CDS             17003..18193
                     /locus_tag="ACHY5W_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446903.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLQASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVSKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSCALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYQMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            18274..18924
                     /gene="inaA"
                     /locus_tag="ACHY5W_002006"
     CDS             18274..18924
                     /gene="inaA"
                     /locus_tag="ACHY5W_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFS4446904.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(18978..19232)
                     /gene="yfaE"
                     /locus_tag="ACHY5W_002007"
     CDS             complement(18978..19232)
                     /gene="yfaE"
                     /locus_tag="ACHY5W_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFS4446905.1"
                     /translation="MASVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(19232..20362)
                     /gene="nrdB"
                     /locus_tag="ACHY5W_002008"
     CDS             complement(19232..20362)
                     /gene="nrdB"
                     /locus_tag="ACHY5W_002008"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4446906.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(20596..22881)
                     /gene="nrdA"
                     /locus_tag="ACHY5W_002009"
     CDS             complement(20596..22881)
                     /gene="nrdA"
                     /locus_tag="ACHY5W_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4446907.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            23009..23152
                     /gene="ypaB"
                     /locus_tag="ACHY5W_002010"
     CDS             23009..23152
                     /gene="ypaB"
                     /locus_tag="ACHY5W_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFS4446908.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         5410 a         5709 c         6231 g         5994 t
ORIGIN      
        1 aaaattttga ggcattttgc cgacatcgta ggattttaat attacaccaa ctgctaatta
       61 tcgccagaaa tgtcgctcaa tttcacggta attgtctggt tgcgcttgtc tataggtgga
      121 gtttacgtgt tagctttttg ccgctcatca ttgaagtcaa aaaaatattt catcatttta
      181 ctggcgctcg ctgcaattgc cggactgggt actcatgccg cctggagtag caatggtttg
      241 ccacgtatcg acaataaaac attggccaga ctggcgcagc agcacccggt tgtcgttttg
      301 tttcgccatg ctgaacgttg cgatcgttca accaatcaat gcttgtcaga taaaacaggt
      361 attacggtta aaggtaccca ggatgcccgt gaactgggca acgcttttag cgctgatatc
      421 cctgatttcg atctttattc cagtaatacc gtccggacca ttcagtcggc gacctggttt
      481 tcagcgggta aaaagctgac ggtagataaa cgacttcttc aatgcggtaa tgagatttat
      541 agtgcaatta aagacttaca aagaaaagca cctgataaaa atatcgttat ttttacccat
      601 aatcattgcc tgacatatat tgctaaagat aagcgtgatg cgacatttaa acctgattat
      661 ctggatggtt tagtcatgca tgtggaaaaa ggcaaagttt atctggatgg ggaattcgtt
      721 aaccactaaa ttttcaatct gacagccagt gatggctgtc atcattgtta aagaagacct
      781 ttcaaacgta acgtttttcg ggtggcaaca ttcaaatcat aatgcaccag atcttcaggt
      841 tttacccacg cgtagtcctg aaactcttcg tttattttca cttctcggtt ggcagaaacg
      901 cagtcaaaaa tcaggtaaat catataaatc tcttccttgc gaccatctgc atacgtcttg
      961 gtgcgaatat catcgctgaa ggtccacggc gtgatttctg tcaaaagcag ctgttctccc
     1021 agttcttcgc gaatttcgcg gcgtagtgcc tcttcaattc gttcgccggg ctccacgccg
     1081 ccacccgaaa gcgcccattg accggggaaa acgccgcgat cgtcggccat tttacacagc
     1141 aaataagcac catcattttg aatcaaaggg catacaatag tccgttgtcg cacaggggta
     1201 ttccttataa ataaaaagaa accatgagaa tacggtaact cagaaatatg tgaaagtgac
     1261 agtaatctct tttgcgatga taactgcact tattattatt tttgtgtgta tcggtaagct
     1321 gtgtttattt tttcggcgtt taattattta agaagtattt agttacaata agtgactaga
     1381 tccctttttt cgctcattga atcctctgcg ccaggcagta caatcccgca gctttgggaa
     1441 ttttgattaa ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc
     1501 cggaatgctg ttggtttctg catcggtcaa tgcaatgagc atcagcggcc aggcgggtaa
     1561 agaatacacc aatattggtg tcggttttgg gactgaatcg acgggcctgg cgttaagcgg
     1621 taactggaca cataacgacg acgacggtga cgtcgcgggc gtggggctgg ggttgaatct
     1681 gcctctcggg ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca acccgaatta
     1741 cggcgatgaa ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag
     1801 cttccgtttg tttggcgagt attactactc tccggattca ctctccagcg gtattaaaag
     1861 ttatgaggaa gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc
     1921 gggttatcgc tacctgaatc tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga
     1981 cggcccgtat gttggggtta acgccagttt ctgatccctt ccggcgcggc attctgtcgc
     2041 gccattcagt ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa
     2101 gcaggagata accatgttaa aagtggaaat gctatccacc ggggatgaag tgttacacgg
     2161 gcaaatcgtt gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggttgcc
     2221 attatctcgc cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga
     2281 acgtagtcag cacgccgatg tgctgatcgt taacggcggg ctgggaccga ccagcgatga
     2341 tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg
     2401 gctcaaagag atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg
     2461 taaacaagcg gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg
     2521 tggttttgcc gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga
     2581 atttaaggtg atggtcgagc acgaaatcct gccgcgtctg cgcgagcgtt tttctttacc
     2641 acagccgccg gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg atctggcaca
     2701 aagcctggac actctacaac tgccgccggg ggtaacaatg ggctatcgct cctcaatgcc
     2761 gatcatcgaa ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg
     2821 gctggacgtt aaacgtgttg ccggacagag cgtgattttc gaaggcaccg aaggactgcc
     2881 cgcgcagatc agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt
     2941 caccggtggt ttattggctt tgcaactttc tcgcgcaggt gctccactgc tggcgtgtga
     3001 agtggttcct tcacaggaag aaaccctggc gcaaactgcg cactggatta cagaacggcg
     3061 ggccaaccat tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa
     3121 ctttgcgcta gccacgccag acggcacttt cgctctgcgt gtgcgtttca gtactacacg
     3181 ctacagcctg gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctacgccg
     3241 ttggttaaac ggccaggaca ttgccagtga gcatggctgg attgaggttg ttgagtccat
     3301 gaccttctcc gtctgattcc gccactctgt gccggtttac tgcaccggca cattccccct
     3361 ccggcaaaat ttgtccaacc tgttgtactc aataaaaaaa atccgtgcgc tgattcacaa
     3421 atttgtgcgt aatttctcca gtccgttgga cgaggagcgg gatttgatgc gcttcacaat
     3481 tttactcccc ctgagcaacg agactgattc cagtatctaa gactggagtc cagtatgttg
     3541 gaatcaagca aagttcccgc cctgacacgg gctattgata ttctcaattt gattgcccgc
     3601 attggcccct gtaatgctgc gacgatcatt gacacattgg ggatccctaa aagcacggcc
     3661 tatttgctgc ttaatgaact caggcgtcag cgttttctta gccttgatca ccaggaaaat
     3721 ttttgtttgt ggaccaaact ggtggagctt tccggtcatg cgttaagcaa gatggatctc
     3781 cgggaactgg ctcgaccgcg tttgacgcaa ctgatggata ccaccgggct gttatgtcac
     3841 ctggggatta ttgataacgg cagcgcttat tacattttga aagtggagtc atcagccact
     3901 atcagcgtgc gttcacatga aggaaaaagc ctttcgcttt atcgctccgg aataggtaaa
     3961 tgcctgcttg cctggcaacc tgcaactgta cagcaaagta ttatcgaagg gctggtctgg
     4021 gagcaggcga cacccacaac gattactcat ccacagcaac ttcacgaaga actggcgcgt
     4081 attcgccgtc agggctggag ctatgacaac ggcgaagatt acgccgatgt ccgttgtgtc
     4141 gctgcgccgg tgtttaacgc caataacgag cttaccgctg cgatctcggt ggtcggtacc
     4201 cgcttacaaa tcaatgaaga ataccgtgat tatctggcgg gcaaggccat tgcctgcgcc
     4261 agggatattt cacgtctgtt gggatggaaa agtccctttg acttacaagc ctcataacaa
     4321 tggagaacat catgacccta ccaaaaatta aacaggttcg cgcctggttt actggcggtg
     4381 cgacagcaga aaaaggcgct ggcggcggtg attatcacga ccagggggcg aatcactgga
     4441 tagacgatca tattgccacc ccgatgagta aataccgcga ttacgagcag tcacgccagt
     4501 catttggcat taacgttctt ggcacgttgg ttgttgaagt cgaagcagaa aacggccaga
     4561 ccggattcgc tgtttcgaca gccggtgaaa tgggctgttt tatcgtcgaa aaacacctta
     4621 accgtttcat tgagggtaaa tgtgtcagtg atatcaaact gatccatgat caaatgctca
     4681 atgccaccct gtattactcc ggctctggtg gcctggtgat gaacacgatt tcctgtgtcg
     4741 atctggctct gtgggatctg ttcggcaaag tggtcgggct tccggtttat aaacttttag
     4801 gcggcgctgt tcgtgatgag attcagttct acgccacagg tgcgcgtccg gatctggcaa
     4861 aagagatggg ctttatcggt ggcaaaatgc cgacgcactg ggggccacat gatggcgatg
     4921 cggggatccg caaagatgcc gctatggtcg cggatatgcg tgaaaaatgc ggtgaggatt
     4981 tctggttaat gctcgactgc tggatgagtc aggacgtgaa ctatgcgacc aaactggccc
     5041 acgcttgcgc gccctataac ctgaaatgga tcgaagagtg cctgccgcca cagcagtatg
     5101 aaggttatcg cgaactgaaa cgcaacgcgc cagtcggaat gatggtcacc agcggtgagc
     5161 accacggcac actgcaatct tttcgtacgc tttcagaaac cggtatcgac attatgcagc
     5221 cggatgttgg ctggtgcggt ggcttaacca cgctggtgga aattgccgca atcgccaaat
     5281 ccagggggca actggtggtg ccgcacggtt cgtctgttta ctcccaccat gcggtgatca
     5341 ccttcactaa tacgccattc agcgaattcc tgatgaccag tccggattgt tcaacgatgc
     5401 gtccgcagtt tgacccgatt ctgctcaatg agccggttcc ggtgaatggt cgtattcata
     5461 aatcagtgct tgataaaccc ggttttggcg tcgaactcaa tcgtgactgc aatttgaaac
     5521 gcccctacag ccactaatca ctccgggcgt tgctgtgaca acggtaacgc ccacatattc
     5581 taaggagctt tttatgagca ccgctttgct tgacgccgtg gtgaagaaaa accgtgtgcg
     5641 tttaattccg tttatgttgg cgctgtatgt gctggcgttt ctcgaccgtt cgaatatcgg
     5701 ttttgccaaa cagacctacc agattgatac cgggttgagt aatgaagctt atgcgctggg
     5761 agcaggcatt ttctttgtgg tatatgcgtt tctgggtgtt ccggcgaatc ttttgatgcg
     5821 caaactgggg gccagaacct ggattggtac gacaacactg ctgtggggat ttctttcggc
     5881 agccatggca tgggccgata ttgaagcgaa atttctgata gttcgcactc tgcttggtgc
     5941 tgcggaggcc ggatttttcc ctggtatgat ttatctcacc tcgcaatggt ttccgcagcg
     6001 taatcgcgcc agcattatgg ggctgttcta tatgggcgca ccgctggcgt taacactggg
     6061 atcaccgctt tccggcgcgc tgctggagat gcatggattt atggggcatc ccggctggtt
     6121 ctggatattt gtgattgaag gattgttggc agtcggcgct ggggtattca cattcttttg
     6181 gcttgatgac acaccggagc aggcacgttt tctgagtaaa caagaaaaaa cgttgcttat
     6241 caatcaactg gcaagtgaag aacaacaaaa agtgacttct cggctgagcg atgcgctgcg
     6301 taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg cggtttacgg
     6361 attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag tgggctttac
     6421 agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct ggcttattcc
     6481 gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat tactggcggc
     6541 gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg tagcgctgtg
     6601 tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc cgacacaact
     6661 gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgtttg gtgcagtggg
     6721 cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca gcgatgcggc
     6781 gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt tcactctgcg
     6841 tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt aaggaacacg
     6901 atgaacgcat tattaagcaa tccctttaaa gaacgtttac gcaagggcga agtgcaaatt
     6961 ggtctgtggt taagctcaac aactgcctat atggcagaaa ttgccgccac ttccggttat
     7021 gactggttgc tgattgatgg ggagcacgcg ccaaacacca ttcaggatct ttatcaccag
     7081 ctacaggcgg tagcccccta tgccagccaa cccgtgatcc gtccggtgga aggcagtaaa
     7141 ccgctgatta aacaagtcct ggatattggc gcgcaaactc tactgatccc gatggtcgat
     7201 actgccgatc aggcacgtca ggtggtgtct gccacgcgct atcctcccta cggtgagcgt
     7261 ggagtcgggg ccagtgtggc acgggctgcg cgctggggac gcattgagaa ttacatggcg
     7321 caagtcaacg attcactttg tctgttagtg caggtggaaa gtaaaacggc actggataat
     7381 ctggacgaaa tcctcgacgt ccaagggatt gatggcgtgt ttattggacc tgcggatctt
     7441 tctgcgtcgt tgggctaccc ggataacgct gggcacccgg aagtgcagcg aattattgaa
     7501 accagtattc ggcggatccg tgctgcgggt aaagcggctg gctttctggc tgtggctcct
     7561 gatatggcgc agcaatgcct ggcgtgggga gcgaactttg tcgctgttgg cgttgacacg
     7621 atgctctaca gcgatgccct ggatcaacga ctggcgatgt ttaaatcagg caaaaatggg
     7681 ccacgcataa aaggtagtta ttgatatcaa aggcccatgg ggatcggctg tgggcctgtg
     7741 ttaattagtg gttattcgct gccagatcgg cttcgcttag ctgggtggcc gcgagcacca
     7801 ggtcacgatc aaagccctgc tccagcatgc gcaaagcaat tttaatggct tgttcatgca
     7861 tgccttcctg caaaccttct aatttacctt gctgcaaacc tactaattta ccttcttgcc
     7921 agccaatttg atgtccttcc tgccgtagcc gttcagcaat agtcatcaat ctctcctttt
     7981 aatgacagtt cggaatatca gcatcagtga gttgggttgc cgccagcaca atctcacgtt
     8041 caaagccctg ctccagcatg cgcaaggcaa ttttaatggc ttgctcatgc atgccttcct
     8101 gcaaaccttc taatttacct ttctgcagac cctccaattt accttgctgc aaaccttcta
     8161 atttaccttc ctgccaacca atttgatgcc cttcctgcct cagccgttca gcaatagtca
     8221 ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa cgggtgaaac
     8281 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     8341 tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     8401 gaatatgctt ttgaatcagt tcaagcagag ctatacgccg atgttgcatg atctcatcgt
     8461 caggtacgat ggtaatatcc accaatggaa aagcttcggt gtacaactgc cgggcaagtt
     8521 gcggatcgtc aaactcatcc agccagttga gtgagtacgg gtagggcgag gtttcgccat
     8581 gataaaacag caacggcacc accagcggga ctctgtcata gcctttgtcc aggtgacgct
     8641 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     8701 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     8761 catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc aggcgtaagg
     8821 tttgcaggtt gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg
     8881 cggttttggg tgtgaacata aaggttttaa acaccgcatc atgcggggag gaggttgttg
     8941 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     9001 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     9061 gttgcaacgc atacactggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     9121 ttaccgggca tcagaaagga gtttaagcca gcgcctgggc cagcagcgta atcggatgtt
     9181 cgcagcgaag gcttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     9241 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     9301 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     9361 gcaccgttaa ctcaagcccc gggatgttac gcaacagctc cagggtgtag agcgtccagc
     9421 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     9481 gtaacgtttt gccttcatcc agcttgcgcc acagccagcg ggtcgccagt tcgatatgat
     9541 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     9601 tacaggttga ggaggtggca atcaccggaa tgccttttac gcccacagct tcgcggatcg
     9661 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgatcagcg
     9721 gtacgccgca gcatttttct ttgctgagca gttgtacacc agtacccatt gcgttgagca
     9781 ctttaattaa atctttgcct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     9841 aagcgacctg gtctttatac tgcgcttgct gagccgccac gctgcgatac cagcgacgga
     9901 acgtgccgaa ggagtatttc ggtagcgtac ggcgatgatc gatttttaac gccgcatcaa
     9961 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
    10021 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
    10081 tgtcatattt cgcccgcgcg cgctggatga tatcgccaat cttcacatcg gacgggcagg
    10141 cgacttcaca acgtttgcag ttgatacaat atttcagcgc ctcgtcatac agtgcgccat
    10201 cttttaaacg cagacgctcg ccatccggcc cggcttgttt tggtcctgga taaccgggat
    10261 tcacccagct cactgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
    10321 tggtgtcatt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
    10381 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
    10441 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgtgatgggc gtagcgtctt
    10501 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatctc ccttatacca
    10561 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctctc gaatgccgtt
    10621 acgttccgct accagtccgc cactaaaaaa actgccgctg gcgagaaccg cgaaacgtgg
    10681 acgcagcggg atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca
    10741 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctgacgca caaactggcg
    10801 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
    10861 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcag ccaggccgaa
    10921 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
    10981 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc ggaactcggt
    11041 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caacggcaag
    11101 gccgagttca cgcaacgaag ctgccgcaag gtgcgcctga aaatccatca gtccgctaat
    11161 tcccactaca catattttct tcgcgggcag cggccagacg gggacttctg gcgaacttag
    11221 ccaggttgag cgcagggtgc cgagcggggt aacacgctgg tgagccagct ccacgctacc
    11281 ctggagttgc gcgccgcttt ccgctatcaa tgcctgcgct tggcaagcga gatcgagcac
    11341 gcgttgtggc ccgagaagag agtaaggatg ggcaggtgcc tgctgacgca aagattccag
    11401 tccactgtga atgtctgtca ccgcttgacc atctggcaga tggctcagca aatccagcga
    11461 accggatgag aaatgcagcg cgctttgacc acgcgtgaca atggcacagc ggaggccgtg
    11521 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgatagtatc
    11581 aaagcgcatc tttctgctcc ttctccagac cacacagtcc ctgataaacc cagcgggtaa
    11641 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    11701 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaccgttgca
    11761 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg
    11821 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    11881 cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca
    11941 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
    12001 gggagatgac tttacgcaag gtgacttcag cgggttcctg tgacccagga agtgccagat
    12061 cggcggtcgt acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt
    12121 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
    12181 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
    12241 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
    12301 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
    12361 ctcgattatc gtcaatctca ttgtaatcaa tacgtaaaga ggtggtgcca attagcgaaa
    12421 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt gcggcagcgg ttgatcacat
    12481 gctgattaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
    12541 gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta accacgacag
    12601 gcgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgtcgcgcc
    12661 ttcacgaatc agtcccgtga cttcatgagc agtaaggata acggcaccgt gttctttggc
    12721 atccagcatg tttgctgcgg tcagacgaaa tggatcaacg gtgccatccg gaactttcac
    12781 cgcgccaatc agtgccgggt taacggcagg ttcgataatg cgcgcttgct gcgggtctat
    12841 agcttctgcg ctgatccctg cttcttcgca ggcgcgaata aaagtggcct ggaaggagag
    12901 gtcatcttcc tgcagggtga taaacaggcc gttagttggt tcaacgcagt gacgtgcaat
    12961 gcgtttcagg atctggtttt cactaatgca ttcgcgggcc gattccgcat cggttactgc
    13021 atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg gtggcaccgg ttgcgatgtc
    13081 gtggcgctca accaaaatca cgcgcagccc gcgcagggca cagtcgcggg caatcccggc
    13141 tcccgttgcg ccgccgccaa tgataatcac gtcacttgat tgcgagtcgc gagttttcat
    13201 tgttatccct ctgaagttcg ttttttacca tttagccata gtaaaaacat gaattgtttg
    13261 atttcgcgca tattcgctca taattcgaaa gtgaaacgtg atttcatgcg tcattttgaa
    13321 cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt atgaatgttt
    13381 tcttaacatc gcggcaactc aagaaacggc aggttctctc actgaatcag gctgttaatc
    13441 ataaataaga ccacgggcca cggaggcttt caatgttgag tatttttaaa ccagcgccac
    13501 acaaagcgcg cttacctgcc gcggagatcg atccgactta tcgtcgattg cgctggcaaa
    13561 ttttcctggg gatattcttt ggctatgctg cttactattt ggttcgtaag aactttgcgc
    13621 tcgctatgcc ttacctggtg gagcagggat tctcacgcgg tgatttaggt tttgcgcttt
    13681 ctgggatctc gatcgcctat ggattttcga aattcatcat gggttcggta tcggatcgct
    13741 cgaatccgcg cgttttcctg cccgcaggtt tgattctggc ggcggcagtg atgttgttta
    13801 tgggctttgt gccatgggcg acgtcgagca ttgcggttat gtttgtactg ttgttcctct
    13861 gcggttggtt ccaggggatg gggtggccgc catgcggtcg tactatggtg cactggtggt
    13921 cgcagaaaga acgtggcggc attgtgtcag tgtggaactg tgcgcacaac gtcggtggtg
    13981 gtattccgcc gctgctgttc ctgctgggga tggcctggtt caatgactgg cacgcggcgc
    14041 tctatatgcc cgctttctgc gccattctgg tggcattatt cgcctttgcg atgatgcgcg
    14101 ataccccgca atcctgtggc ttgccgccga tcgaagagta caaaaatgat tatccggacg
    14161 actataacga aaaagcggaa caggagctga ccgcgaagca aatcttcatg cagtacgtgc
    14221 tgccgaacaa actgttgtgg tacatcgcca tcgccaacgt gttcgtttat ctgctgcgtt
    14281 acggcatcct cgactggtca ccgacttatc tgaaagaggt taagcatttc gcgctggata
    14341 aatcctcctg ggcctacttc ttctatgagt atgcgggtat tccgggcacc ctgctgtgcg
    14401 gctggatgtc ggataaagtc ttccgtggca accgtggggc aaccggcgtt ttctttatga
    14461 cactggtgac catcgcgact atcgtttact ggatgaaccc ggcaggtaac ccaaccgtcg
    14521 atatgatttg tatgattgtt atcggcttcc tgatctacgg ccctgtgatg ctgatcggtc
    14581 tgcatgcgct ggaactggca ccgaaaaaag cggcaggaac ggcagcgggc tttaccggtc
    14641 tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat tgttggctac accgtggact
    14701 tcttcggctg ggatggcggc tttatggtaa tgattggcgg cagcattctg gcggttatct
    14761 tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatca ggaattactg caaaaacgca
    14821 acggaggcta atggcatgaa attgacgctg aaaaacctta gcatggcgat catgatgagc
    14881 actatagtca tgggaagcag tgcaatggcg gcggacagca acgaaaaaat agtcatcgcc
    14941 catcgcggtg ccagtggata tttgccggag catacgctgc cagcaaaagc gatggcgtat
    15001 gcgcagggag cggattatct ggaacaggat ttggtgatga ccaaagacga ccatctggtt
    15061 gttctgcatg accattacct cgatcgtgtt actgatgttg ccgatcgttt cccggatcgg
    15121 gcgcgcaaag acggtcgtta ctacgcgata gatttcactc tggatgaaat taagtcgttg
    15181 aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag tgcagactta tccagggcgt
    15241 ttcccaatgg gtaagtccga cttccgggtg cacacctttg aagaagagat tgaatttgtt
    15301 caggggttaa atcactctac cgggaaaaat atcggtatct atccagaaat caaagcgccg
    15361 tggttccatc atcaggaagg gaaggatatt gcggcaaaaa cgctggaagt gctgaagaaa
    15421 tatggttaca ccggtaaaga cgataaagtt tatttgcaat gttttgatgc tgatgagctg
    15481 aagcgtatta agaatgagct ggaacccaaa atgggcatgg atctcaatct ggtacagctg
    15541 attgcctata ccgactggaa tgaaacgcag cagaaacagc cggatggaag ctgggttaat
    15601 tacaactacg actggatgtt taagccgggt gtcatgaaac aggtggcgga atatgcagat
    15661 ggtattggtc cggattacca tatgttgatt gaggagacat cgcagccggg taatatcaaa
    15721 ctcactggca tggtgcaaga tgctcagcag aacaagctgg tagtgcatcc ttataccgtg
    15781 cggtcagata aactgcctga atacacaact gatgtgaatc agttatatga tgctctatat
    15841 aacaaagcgg gtgtaaatgg gttgtttact gatttccctg ataaagcggt taaattcctg
    15901 cataaagata attaatgaca caggcatccg ggaaggcaaa acctgaccgg atgccctttt
    15961 cacaatatgc ctttgaccac cagggtttcc atacacattt gcaataaaaa caatgggctg
    16021 ccttctaccg gcgcctcttt gaccagcaat gaaatttgcc gataaaacgt cggctccagc
    16081 gggacagagc ataactcacc ggcgacggct ttcagcgtca gctccggtaa cagagcaatt
    16141 cctaatccct ggcgaataaa acttattgcc gtatctggat ggttaaattc atatttaatg
    16201 accggcgtaa tattcttttc cttaaataac gccataatgc ttaattcata acgcccctta
    16261 ctgataatga gcggttctcc caccagttct tccaccgata tagcgttatt ttttgccagg
    16321 ggatgagcat ctggcagcac cacggtaaat ttatcctgat ataccgggac ggaatacatt
    16381 ccggttaccg gaaaatggac aaaaccggca tcgatagttt cgttttgcac cgaatcaata
    16441 atttcactgc tgttctcttc gtaaggaatg atttttatct gcggatgata agtttcaaaa
    16501 tagcgaataa tacccggtaa aatacacgcg caggcgctgg gaaagcagcc gatgcgcagc
    16561 gttctggtcg ggttatgttt ttcctgctcg gcaatttctt tcacagcgtt cacttcacgg
    16621 gtaatcgccc gcagatggct tgcgatgcgt gagccggcgt cagtcagctg cagtgctttt
    16681 cgccgctcgc ggatcaggat ctctacgcca agctcttgct ccagggtggc aatggcctgg
    16741 cttacggcgg attgcgtcat aaacaggcgc ttactggctt cggtaaagcc gccataatcc
    16801 agtacggcga gcagggtgtg gatttgggtt agtgtcatta gtaatatcta attaatatga
    16861 ttaaaacgat taatttctct tatagagtgc atccatttat actctatttc tatcgagtaa
    16921 atcacatttt gacgcacctg tagtggttca gcctgtgatg ctgagcggtg tccttgtgcg
    16981 ccatttgaac gggtcgttta gtatgaaaga aaaattatgg acgaaagatt tttgggcaat
    17041 caccattatt agctttatta tctttttcgt cttttatgtg ttgctgacct tattacctat
    17101 ttatatctcc gatcgtctcc aggcatcccc tgataaagct ggtctgctgg tgactttatt
    17161 tttaattgcg gcgattgtta ttcgaccctt tgccgggcaa tgggtgagta aatattcgaa
    17221 taaaactatt ctggtgctct cttctctggc ctttttggtg gtcactgcgc tgtatccttt
    17281 ttgccactca atagaatcac tgctttttat tagggtgctt catggtatta ccttcggggt
    17341 tatcacaacg gtaaagggaa cgatttccgc gcggctgatc ccggcctccc gacgtgggga
    17401 gggcatcagt tttttctctc tggcaatggg gctggcaatg gtggtcgggc cgtggattgg
    17461 cctgaatatg gcgcgctggg aggcctttaa tatggctttc tggttatgta ctggcgttgc
    17521 ggcggtgggg attatcctgt cgctgattat gaccgtgccg ccggttatca gccatgccga
    17581 cggttcaaag ccaaagatgg gcttcgccgc catgttcgat cgcgcggcat tgccgtttgc
    17641 catggttaca ttctttatga ccttttcgta tgccggggtt tctgcctttc tggcgcttta
    17701 cgcccgcgaa cttaatctga tgtcggcggc cagtaatttc ctgctctgct acgccatctt
    17761 cctgatgatc tgccgtacct tcaccggcaa tgtttgcgac aaaaaaggcc cgaaatatgt
    17821 ggtttacccc tgcctgctgt tctttacggt tgggctggtg gttctcggct acacccaggg
    17881 cagcgtaatg atggtcgttt cttgcgcgtt gattggtatc gggtatggtt ccgtgacgcc
    17941 agtttttcag acgcagatta tcagttcagt ggaaccgcat aaaatcggtg tcgcaaactc
    18001 cctcttcttc aatgcgatgg atgcaggcct ggcgctggga gcctgtgtga tggggatgat
    18061 ggttgcacat actggctacc aaatgattta tctgctgggc gcactattag tggtagtggc
    18121 tggtggagtc tatgcgctgc aaatgaaggg taaaagcggt gtcgcgctag tagtggcaaa
    18181 agaaattcat taatacgaca cgtttcatta agatttttcg caggtaacca cctatagtca
    18241 ttgtcgttaa taaaaaatga caggcgatag ggtatggcag tttcagcaaa gcacgacgaa
    18301 tttaatcact ggtgggcaac agagggcgac tgggttgaag agcctaacta tcgccgtaat
    18361 ggtatgagcg gggtgcaatg cgtcgagcgc aacggcaaaa agctatatgt aaagcgcatg
    18421 acgcatcatc tgtttcattc cgtacgttat cctttcggcc gaccaacgat tgtccgtgag
    18481 gttgcggtaa ttaaagaact ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt
    18541 gaagcggtga aaattgaggg ggaatggcgc gcgttgctgg tgactgaaga tatggcgggg
    18601 ttcatcagca ttgctgactg gtatgcccag catgcagtat cgccttattc tgacgaagta
    18661 cggcaagcca tgttgaaagc agtggcgctg gcgtttaaga aaatgcatag cattaatcgt
    18721 cagcatggct gttgttatgt tcgccatatc tatgtgaaaa cagaaggtaa ggcagaagct
    18781 ggttttcttg atctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat
    18841 gacttccgcc agctggaaaa atatctggag ccgatcccca aagcggactg ggagcaggta
    18901 aaagcgtatt actacgccat gtaatcgttg taggccagat aatacgcgta agcgtcgtat
    18961 ctggcgctag tacccgatca caactcgatt tcaatatcgc cttttgcccg gcaacaacag
    19021 ggcaaaattt cccccggctg aataaaagct aacggttcgg caatccagtc cacctggcct
    19081 gcgaccagtc gggtgcgaca ggagccgcag taaccttcgc ggcactggta ctcaaccgcc
    19141 acattgtggg attccagcgc cgccagaagg gaagggtgtt catcctggca cagcagttgt
    19201 gtgccagtga tgcgcagggt aacgctagcc atcagagctg gaagttactc aaatcgtcgg
    19261 tgtccacttc cgagtcaatc tgcccgacca gataagaact gacttccact tcctgcggag
    19321 caacctgcac gttatcagac accagccagg tgttgatcca cgggatcggg ttggagcgcg
    19381 tctggaacgg cagatccaaa ccgactgcct gcatgcggat attggtgatg tattcaacgt
    19441 actggcagag aatgtcttta ttcagaccaa tcatcgaacc gtcacggaac agataatccg
    19501 cccagtcttt ctcttgctgt gccgcctgga caaacaggtc atagcattcc tgcttacatt
    19561 cttcggcaat ttccgccatc tccggatcat ccgcaccgct gcgcagcaga ttcagcatat
    19621 gctgagtacc ggtcaggtgc agggcttcgt cgcgggcaat caggcgaata attttggcgt
    19681 taccttccat cagttcgcgt tctgcaaagg caaaggaaca agcaaagctg acgtagaagc
    19741 ggatcgcttc cagcgcgtta acgctcatca ggcagagata cagttttttc ttcagctcgc
    19801 gcaggctaac ggtcacggtt ttaccgttaa cagtgtgggt gccttcgccc agcaggtgcc
    19861 agtagctggt catttcgatc agctcatcgt agtagctgga gatcccttcc gcacgtttct
    19921 ggatctgctc gttggtgacg atatcgtcaa acacaactga cggatcgtta acgatattac
    19981 gaatgatatg agtataggaa cgggaatgaa tcgtttctga gaacgcccag gtttctaccc
    20041 aggtttccag ttccgggatc gagatcagcg gcagcagtgc cacgttcggg ctacgaccct
    20101 gaattgaatc gagcaacgtc tggtatttca ggttgctgat aaaaatgtgt ttttcgtgtt
    20161 caggcagcgc ctggtaatcg atacggtcac gagaaacgtc aacttcttcc ggacgccaga
    20221 agaaagagag ctgcttttcg atcagctttt cgaagatgtc atatttttgc tgatcgtagc
    20281 gagccacgtt gaccggctga ccaaagaaca tcggttcttt gagctgatca tttttcgtct
    20341 gtgaaaaggt ggtatatgcc atgagtgtgt cctgttggga gtttaatgcc ggataaggca
    20401 ttttacgccg catcctgtgc ctgatgcgac gctggcgcgt cttatcaggc ctacaaatcc
    20461 gagtcgtagg ccggataagg cgttcacgct gcatccggca cccggagcct gatgcgacgc
    20521 tggcgcgtct tatcaggcct acaaaccgag ccgtaggcct gataaggcgt ttacgccgca
    20581 tccggcatct caatatcaga tcttacatgc gccgctttcg cagccatcgt cctggattga
    20641 cggcaccaga tcgtcctgcg cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt
    20701 tttgaccccg aatttgtagg cggtgagcag gtctttcagc aactgctgca tcggcacttt
    20761 tcctgacggg aagcgtgacg gatcgtagtt ggtgttagca gagatcgact gatcgataaa
    20821 tttctgcatg atacctacca gttgcaggta gccatcgtta cccggcattt cccacagcag
    20881 ctcataggcg tcgtgcagat gctcgtagtc cggcaccacc tggcgcagaa taccgtcttt
    20941 cgacgctttg atgctgacgt aaccgcgcgg cggttcaata ccgttagtgg cgttagagat
    21001 ctgtgaagaa gtctcggacg gcatcagggc agaaagcgtg gagttacgca gaccgtgcgt
    21061 tttgatcgat tcacgcagtg cttcccagtc gtaatgcagt ggttcattag cgatggcatc
    21121 cagatctttc ttataggtat cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa
    21181 ccacgggcac gcgccttgct ctttcgccag ctcattagac gctttcagca ggtaatactg
    21241 aatcgcttcg aaggttttat gcgtcaggtt gttggcgctg ccgtcggagt aacgtttacc
    21301 gtgcttcgcc aggtagtaag cgaagttgat cacaccaata cccagcgtac gacgacccat
    21361 cgctccacgt ttggcggccg ggatcgggta atcctgataa tccagcagcg cgtcaagtgc
    21421 acgaaccgcc agaattgcca gctcttccag ttcatccagg ctattaattg cgcccaggtt
    21481 gaaagcagac agcgtacaca gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg
    21541 tttggtcggc agggctatct ccaggcacag gttagactga cgcactggcg cgatggccgg
    21601 atcaaacggg ctatgggtat tgcagtggtc aacgttctga atatagatac gaccggtaga
    21661 tgcacgttcc tgcatcatca gcgagaacag ctcaacggct ttcacacgct gcttgcggat
    21721 gctgtcgtct ttctcatatt tggtatacag gcgttcaaac tcttcctgat cggcgaagaa
    21781 tgcgtcgtac agccccggta cgtcggacgg gctgaacagg gtgatatctt cacctttcag
    21841 caggcgggta tacatcagtt tgttgatctg taccccgtag tccatatggc gcacgcggtt
    21901 gccttccaca ccacggttgt ttttcaacac cagcaggctt tccacttcca gatgccacat
    21961 cgggtagaac agcgttgccg caccgccgcg cacgccgccc tgagagcagg atttcaccgc
    22021 agtctggaaa tgtttgtaga acggaatgca gccggtatgg aacgcttcgc caccgcgaat
    22081 cgggctaccc agcgcacgaa tacgcccggc gttgatgccg atcccggcac gctgggaaac
    22141 atatttcaca atcgcgctgg aggtggcgtt gatggaatcc aggctgtcgc cgcactcgat
    22201 cagcacgcag gagctgaact gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg
    22261 cagcgaaatt ttaaatgtgg aaaccgcgtc gtaaaaacgc ttcacatatt gcaggcgcgt
    22321 ttcacgcggg tagttcgaga acaggcacgc ggcaaccaga atataaagga actgggcgct
    22381 ttcgtagatt tcgccggtca cgcggttctg taccagatat ttgccttcca gttgcttaac
    22441 ggcagcataa gagaaggtca tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa
    22501 ctcttcttcc gtgtagtctt ccagcagatg attatcgtat ttgcccatct cgaccatttt
    22561 caccacgtgg tcgtacagcg caggcggctc aaactggccg taggcttttt tacgcaggtg
    22621 gaagatcgcc aggcgcgcgg cgagatactg ataatccggc gcatcacggg agatcaggtc
    22681 tgcggcagcc ttgataatag tttcgtggat gtcagaggtc ttgataccgt cataaaactg
    22741 aatgtgggag cgcagctcga cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca
    22801 atccagaacg cgatggattt tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt
    22861 caccagcaga ttctgattca tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt
    22921 ttcgtgcaga ctacctgtag tacgggaagc tgcttagaac actatatata gggggtatgt
    22981 ttgggattca ctgcaagata gtgtgaaaat ggccctcttg caagtgcata actttgtgga
    23041 taactcagga aggaaaaagt ggctttcgcg caccttaggt cagacaaggt gtccgggaaa
    23101 gtcaatggga agagaaaaat ttgttaaaaa taactgttcg gcgatttctt gaacggcaga
    23161 ataggttgga taaattgata tagatggttt attaatcttt ttgctaatga ttgcaaaaat
    23221 tgatctaagc acgagtcatc acataatctg atgtatcagt tgtggtcaat atataagcga
    23281 atgtaagcga ttgttgatgt cgaaatggtt gttttctatt cggttattgt taaatgcgaa
    23341 gcca
//
LOCUS       DAZUGK010000060        23200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZUGK010000060 DAZUGK010000000
VERSION     DAZUGK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.060"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1047
                     /locus_tag="ACHY5W_002011"
     CDS             <1..1047
                     /locus_tag="ACHY5W_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFS4446909.1"
                     /translation="KPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNIL
                     NNNKKKTAILLLALIPFLFFPVLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKIL
                     VCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEV
                     SSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVM
                     LVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSA
                     QAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALRE
                     AKSEGGNKVIIHHI"
     gene            1228..2718
                     /gene="dgcJ"
                     /locus_tag="ACHY5W_002012"
     CDS             1228..2718
                     /gene="dgcJ"
                     /locus_tag="ACHY5W_002012"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFS4446910.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIASQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2761..3264
                     /gene="yeaK"
                     /locus_tag="ACHY5W_002013"
     CDS             2761..3264
                     /gene="yeaK"
                     /locus_tag="ACHY5W_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFS4446911.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     INFRRTA"
     gene            complement(3265..3369)
                     /gene="yoaI"
                     /locus_tag="ACHY5W_002014"
     CDS             complement(3265..3369)
                     /gene="yoaI"
                     /locus_tag="ACHY5W_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFS4446912.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3539..3985
                     /gene="yeaL"
                     /locus_tag="ACHY5W_002015"
     CDS             3539..3985
                     /gene="yeaL"
                     /locus_tag="ACHY5W_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4446913.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3942..4763)
                     /gene="nimR"
                     /locus_tag="ACHY5W_002016"
     CDS             complement(3942..4763)
                     /gene="nimR"
                     /locus_tag="ACHY5W_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFS4446914.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4860..6041
                     /gene="nimT"
                     /locus_tag="ACHY5W_002017"
     CDS             4860..6041
                     /gene="nimT"
                     /locus_tag="ACHY5W_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFS4446915.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQIGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAMLSLLMAIFGLCAGRDKEI
                     R"
     gene            6096..6443
                     /gene="yeaO"
                     /locus_tag="ACHY5W_002018"
     CDS             6096..6443
                     /gene="yeaO"
                     /locus_tag="ACHY5W_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4446916.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6465..6719)
                     /gene="yoaF"
                     /locus_tag="ACHY5W_002019"
     CDS             complement(6465..6719)
                     /gene="yoaF"
                     /locus_tag="ACHY5W_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFS4446917.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPDAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6902..7927
                     /gene="dgcP"
                     /locus_tag="ACHY5W_002020"
     CDS             6902..7927
                     /gene="dgcP"
                     /locus_tag="ACHY5W_002020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFS4446918.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7960..8058
                     /gene="yoaK"
                     /locus_tag="ACHY5W_002021"
     CDS             7960..8058
                     /gene="yoaK"
                     /locus_tag="ACHY5W_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFS4446919.1"
                     /translation="MRIGIIFPVVIFITAVVFLACFFIGGYAAPGA"
     gene            8061..8135
                     /gene="yoaJ"
                     /locus_tag="ACHY5W_002022"
     CDS             8061..8135
                     /gene="yoaJ"
                     /locus_tag="ACHY5W_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFS4446920.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8194..8442)
                     /gene="yeaQ"
                     /locus_tag="ACHY5W_002023"
     CDS             complement(8194..8442)
                     /gene="yeaQ"
                     /locus_tag="ACHY5W_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4446921.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8590..8772)
                     /gene="yoaG"
                     /locus_tag="ACHY5W_002024"
     CDS             complement(8590..8772)
                     /gene="yoaG"
                     /locus_tag="ACHY5W_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFS4446922.1"
                     /translation="MSKATYTVTVTNNSNGVSVDYETETPMTLLVPEEAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8776..9135)
                     /gene="yeaR"
                     /locus_tag="ACHY5W_002025"
     CDS             complement(8776..9135)
                     /gene="yeaR"
                     /locus_tag="ACHY5W_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFS4446923.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9308..9947)
                     /gene="leuE"
                     /locus_tag="ACHY5W_002026"
                     /pseudo
     CDS             complement(9308..9947)
                     /gene="leuE"
                     /locus_tag="ACHY5W_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
     gene            complement(10074..10996)
                     /gene="dmlR"
                     /locus_tag="ACHY5W_002027"
                     /pseudo
     CDS             complement(10074..10996)
                     /gene="dmlR"
                     /locus_tag="ACHY5W_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
     gene            11099..12184
                     /gene="dmlA"
                     /locus_tag="ACHY5W_002028"
     CDS             11099..12184
                     /gene="dmlA"
                     /locus_tag="ACHY5W_002028"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFS4446924.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12435..14045
                     /gene="yeaV"
                     /locus_tag="ACHY5W_002029"
     CDS             12435..14045
                     /gene="yeaV"
                     /locus_tag="ACHY5W_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFS4446925.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSTDAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14077..15201
                     /gene="yeaW"
                     /locus_tag="ACHY5W_002030"
     CDS             14077..15201
                     /gene="yeaW"
                     /locus_tag="ACHY5W_002030"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFS4446926.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLLESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15257..16222
                     /gene="yeaX"
                     /locus_tag="ACHY5W_002031"
     CDS             15257..16222
                     /gene="yeaX"
                     /locus_tag="ACHY5W_002031"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFS4446927.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSNVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGICG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16276..17391)
                     /gene="rnd"
                     /locus_tag="ACHY5W_002032"
     CDS             complement(16276..17391)
                     /gene="rnd"
                     /locus_tag="ACHY5W_002032"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFS4446928.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHNISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17473..19158)
                     /gene="fadD"
                     /locus_tag="ACHY5W_002033"
     CDS             complement(17473..19158)
                     /gene="fadD"
                     /locus_tag="ACHY5W_002033"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFS4446929.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19363..19944)
                     /gene="yeaY"
                     /locus_tag="ACHY5W_002034"
     CDS             complement(19363..19944)
                     /gene="yeaY"
                     /locus_tag="ACHY5W_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFS4446930.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHFTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(19984..20679)
                     /gene="tsaB"
                     /locus_tag="ACHY5W_002035"
     CDS             complement(19984..20679)
                     /gene="tsaB"
                     /locus_tag="ACHY5W_002035"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4446931.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20737..22647)
                     /gene="yoaA"
                     /locus_tag="ACHY5W_002036"
     CDS             complement(20737..22647)
                     /gene="yoaA"
                     /locus_tag="ACHY5W_002036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4446932.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGELPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESRFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22779..23123
                     /gene="yoaB"
                     /locus_tag="ACHY5W_002037"
     CDS             22779..23123
                     /gene="yoaB"
                     /locus_tag="ACHY5W_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS4446933.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5684 a         5931 c         5641 g         5944 t
ORIGIN      
        1 aaaccaatta acggcagtac actgatccag acaaagtcga atgatgtttc tattttctat
       61 attttccgcc aactcagttt tatttgttta acctcgctgg cgctcttttg ttatggcaaa
      121 gacaacatcc tgaacaataa taagaaaaaa acggcaatcc tgttgctggc gctgatccct
      181 tttttatttt ttcccgttct ggcacacaat ctgagcagtt ataacgctga ctattctttg
      241 tatgtcgtcg attactgtcc tgacaaccat actgcgacct ggggaatcaa ctatacaaaa
      301 atattggttt gtctgtgggc atttttactg ttctttatta tcatgcgtac acgattagcc
      361 agcgaactat ggccattaat agcattatta tgtctggcat cgctatgctg caacttactt
      421 ctactgactc tggatgagta taattacacc atctggtaca tcagtcgcgg gattgaagtc
      481 tccagtaaac tgtttgttgt gtcttttctg atttataaca tttttcagga gctgcaactc
      541 tccagcaaac tggcagttca tgatgtgctg accaatattt ataatcggcg ctactttttc
      601 aacagcgtag agtcattatt gtcgcgacct gttgttaagg acttttgtgt catgttggtt
      661 gatattaatc agttcaaacg catcaatgcc caatggggac atcgtgtggg tgataaagtg
      721 ctggtatcaa ttgtcgatat tatccagcaa agcatccgcc ccgatgatat tttagcgcga
      781 ctggagggtg aggtgtttgg cttgctattt accgaactca atagtgccca ggcaaaaatc
      841 attgcggaac gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa cagatatgat
      901 gttcctgaac aaatgaccat cagtattggc actgtttttt caacgggtga cacgcgtaat
      961 atctcgcttg tcatgacgga agcagataaa gccttacgcg aagcgaaaag cgaggggggc
     1021 aacaaagtga ttattcatca tatttaagtg caaaaatatt cagagccatg ctttttgcgt
     1081 ggcttttgca tacaatttat tactattacc cctaaatttc ccctccccag cggcgcagag
     1141 atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg ttttctcgtt
     1201 tcactaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct ccggcatttt
     1261 atcgccgcaa gtgtcattgt gctgacatct tccttcctta tttttgaact tgtcgccagc
     1321 gacagagcaa tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt
     1381 tatgataagt atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag
     1441 cagtcggaag tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat
     1501 aatacccatg gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa
     1561 accgcatcca ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat
     1621 caggcagtgg aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa
     1681 gagaaatttc actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt
     1741 aatgttgaat actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc
     1801 gatcgcaaag atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag
     1861 gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc cggtgtatgt tgcagggcag
     1921 ctaaaaggga ttgtgctgct ggatattaac aaaaacaatc tgcggaatat cttttacact
     1981 catgaccgcc ctctcctctg gcgttttctc aatgtcacgc taaccgatac agattcgggg
     2041 cgcgacatta tcatcaacca gagcgaagat aatctgttcc agtatgtcag ttacgtccat
     2101 gacttaccgg gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct
     2161 tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtgcgg
     2221 atgcacttcc gcttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga
     2281 ctgtataatc gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa actggtgcaa
     2341 tccggttctt cggtgatgtt tattgcaatt gacatggaca agttaaagct aataaatgac
     2401 accctcggtc atcaggaagg ggatttagcg attacgttgt tagctcaggc gattaaacag
     2461 tcgattcgta aaagtgatta tgccatccga ctcggtggcg atgaattctg catcattctt
     2521 gtcgattcga cgccgcaaat tgcatcacaa ctgcctgaac gtatcgaaaa acgtctgcaa
     2581 catatcgcgc cgcagaaaga gatcggcttc tcttccggta tttacgcgat gaaagaaaac
     2641 gatacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa taagcagaac
     2701 aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca ggagcatgaa
     2761 atgactgaaa tggctaaagg aagcgtgacg catcagcggt taatcgcttt attatcacaa
     2821 gaaggtgcag acttccgcgt cgttacccat gaagcggtag gtaaatgtga agcggtgtca
     2881 gaaattcgtg gcaccgcttt aggccagggg gctaaggcac tggtatgtaa agtcaaaggc
     2941 aatggcgtaa atcaacatgt tctggcgatc ctcgctgcgg atcaacaggc cgatctgagc
     3001 caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc ggaagtcgat
     3061 gaactcacag gctgtgtctt cggcgcgatc ccccctttca gcttccatcc aaaactcaaa
     3121 ctggttgccg accccctact cttcgaacga tttgatgaaa tcgccttcaa tgcaggcatg
     3181 ctggataaat ccgttatttt gaaaaccgcg gattatttgc gcattgcgca accagaactg
     3241 atcaatttcc gccgcactgc gtaactagcc tgtccgttca ataagcagaa cggacaaaac
     3301 cagcacaaca gcaatggcaa aaaacgatga cgtgataatc agtgtctcga cgaacatttg
     3361 atcgttcata gcatgccccc gggagatagc tgttttcacg ttattgttag cgtgcacaaa
     3421 tggcagtttg atgacagttc gcctgatttt ttatgcaaaa aacgtaaaga ttttctactt
     3481 ccttcctgca gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat
     3541 gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa
     3601 caccactgtc gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac
     3661 ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg
     3721 cgttatggcc cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct
     3781 gaactggaaa tcgctggtgg caattgcagt aggcgttatt gtctcctggc tgggtgggcg
     3841 cggcgtgacg ttaatgggca gccagccgca actggttgct gggctgctgg tgggcactgt
     3901 tttaggcgta gcgctgtttc gcggcgtacc ggttggcccg cttattgccg cgggtctggt
     3961 ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc
     4021 aggcctttct taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca
     4081 actttctgta cagtatcgcc tttcactaat ccctgaagcg ccataatcag ctgtaactgc
     4141 tgacgccact ggcgaaaact caaccccgtc tcttttacta tcagtcgggc aagattgcgt
     4201 tcactcatcg caaaaaagcc agcccattgc cccaatgacc cccactcgac gggctctttc
     4261 gccatcatct ccaccatcgt gcggatttta ggatgagaag aaacgggcaa gtgcagctgt
     4321 tgttgtggct gctgagggag ttcatcaaac aggacctgaa ttaatctgcg ggtcatcggc
     4381 tcagcacgct gagtcgtagt cctgtttgcc agggttaaaa tcaattcccg gcataagggc
     4441 gatattttca atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga
     4501 aaacagagtt cagcgttagc tgtcacttga ttgctatgct tcaccccgcc cggtatccaa
     4561 accgcatatt gtggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca
     4621 tgtaatgcta gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc
     4681 gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca
     4741 ttgagattca acctgtgcat cattttgtcc gaatatagcg ataatttgtc attttagctt
     4801 gattcaacat aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata
     4861 tgacctgttc aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga
     4921 tgattgccac tacattacgc gtcaccttca ccggcgctgc accgttactc gatacgattc
     4981 gttccgctta ctcgctgacg actgcgcaaa tcggcttatt gaccaccctg ccattattgg
     5041 cctttgcgct aatctcacct ttggccgccc cggtggcgcg acgttttggt atggaacgta
     5101 gcctgtttgc cgcgttactt ttgatctgtg ctggtatcgc aattcgctct ctcccttcgc
     5161 cttacttatt atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct
     5221 tactgccagg attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat
     5281 attccctgac aatgggagct gcggcggcac tgggatcggc tatggtagtg ccgctggctt
     5341 tgaacggttt tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct ctgctggctc
     5401 tttttttatg gctgccacag tggcgaagcc aacaacatgc aaatttgagt acttcacgcg
     5461 ccttacatac tcggggtatc tggcgttcgc cgcttgcctg gcaggtaaca ttgtttcttg
     5521 ggatcaactc actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc
     5581 acggctatag cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag
     5641 ctcccggttt gctgatcccg cttttcttac atcatgtgaa agatcagcgt ggtattgcag
     5701 cgttcgttgc cttgatgtgc gcagtgggcg cggttgggct ctgctttatg ccagcgcacg
     5761 cgatcacctg gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga
     5821 cgttcattgg tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac
     5881 aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct gatgggtaaa atacacgatg
     5941 ctaacggtaa ctggtctgta ccactgatgg gtgttgccat gctttcacta ctgatggcga
     6001 ttttcggact ttgcgccgga agagacaaag aaattcgcta atatccggtg ctatagtgac
     6061 gtgacaaatc atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt
     6121 atgatccggc agaacagagc gatggttatc gcatactggt cgaccgcctc tggccgcgcg
     6181 gtatcaaaaa aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg
     6241 aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc aacctttcgc gagcaatatc
     6301 ttgcagaact ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac
     6361 agccgctgac cctgctctac tcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg
     6421 ccgactggct acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc
     6481 ggatggtccc gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa
     6541 ttgttgctga ccccttgcgc tgtctgcact ggaatgagcg tcccgccctt ctgctggcaa
     6601 tagaccgacg ccggatttgc cataccaatc tgcggcggtt taggtgcatc tggctgagaa
     6661 ggggttgaac aaccagccag gaccagcaag caaggcagaa caaaactgat aattttcatt
     6721 tattgatctc acatatttat ccaagcttag agtatcgcgg tatcgttttg ttttgcagca
     6781 ctatttttat tacattcact caaaacatat tacgtcttgt ttcatctttg ttgatgatgt
     6841 tttatcatgc ctgcaaagat taaataatca gcacttaccc gccgtatcct ggagttgttc
     6901 cgtgtcagat cagattatcg cccgcgtctc gcaatccctt gccaaagaac agtcactgga
     6961 aagtctggtc cgacagcttc tggagatgct ggaaatggtc actgatatgg aatcaaccta
     7021 cctgaccaaa gtggatgtcg aagcgcgcct gcagcatatt atgtttgccc gtaacagcca
     7081 gaaaatgcac atcccggaga attttaccgt ctcgtgggat tactcgttat gcaaacgcgc
     7141 cattgatgaa aactgctttt tcagcgatga agtccctgac cgttggggcg actgtattgc
     7201 ggcacgcaat cttggcatca ccacatttct gagcacgcca attcacttac cggatggatc
     7261 attctatggc acgctttgcg ccgccagcag tgagaagcgc cagtggagtg aacgcgcgga
     7321 acaggtttta cagttattcg ccggactgat tgcacaatat attcaaaaag aggcactggt
     7381 tgaacagctg cgcgaagcca atgctgcgct gattgcgcaa tcgtataccg actcgttaac
     7441 cgggctaccg aatcggcggg cgatttttga aaatctgacg acgctgtttt ccctcgcccg
     7501 gcatcttaac cataagataa tgatcgcgtt tatcgatctg gataacttca aattaatcaa
     7561 tgatcgtttt ggtcataata gtggcgatct gtttctcatt caggttggcg agcgccttaa
     7621 tacgctccag caaaatggcg aagttattgg tcgtctcggt ggtgatgagt ttttggttgt
     7681 ttcactaaac aacgagaatg cggatatttc gtcgctgcga gaacgtattc aacagcaaat
     7741 acgtggagaa tatcacttag gtgatgttga tttgtattat cccggtgcca gtcttggcat
     7801 agtagaagtc gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg ccgatatcgc
     7861 gatgtatcag gagaaaaaac acaaacagaa aacacctttt gtcgcgcatc cagcgctaca
     7921 ttcctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg tattattttt
     7981 ccggttgtaa tcttcattac agcggtcgta tttttagcat gtttttttat tggcggctat
     8041 gctgccccgg gagcataaag atgaaaaaaa caacgattat tatgatgggt gtggcgatta
     8101 ttgtcgtact cggcactgag ctgggatggt ggtaacgtca cctctaaaaa atagcaaagg
     8161 ctgcctgtgt gcagcctttg tgcaatttaa gcgttaactt ttaatcttcc tgtagataaa
     8221 tagcacgaca atcgcaccaa taacggcaac cacgaagctg ccaaaattga agccatcaac
     8281 tttaccaaag ccaaacagcg tgctgatcca tccgccgact acggcaccga ctatccccag
     8341 caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact tcgccagaat
     8401 accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct cacttatctg
     8461 ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcggg agggcaagat
     8521 ttgtgacgag catcacggag gatttttttg cgatggcgca gaaattgcgc catcaacgat
     8581 cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcataag aacgtacggt
     8641 attcaccaga tcttttatca cttcagccgc ctcttctggc accagcaaag tcatcggcgt
     8701 ctctgtttca taatcgacag aaacgccatt gctgttattg gtgacggtca cggtatacgt
     8761 tgctttgctc atgattcatt tcccgttatg aatgactttc cgttgttgcg caccttccat
     8821 caggacttca ggagccacga agaagtcaat attgaaataa gtatcgtcag tcatggcttc
     8881 aatgttgtgc cacttttctg gagggaacac cgcgaactgc cccgcttcga taaggatcac
     8941 ctgatcaggc tctgcactgt gttcatcagc gtagccgaga tatttgaccg ccccatgcat
     9001 aacggaaagg cgtggataaa cccccgggcg cgttccttta tcaagatgac gttcgaatat
     9061 tccggcaggc gcagtttgtt tattccagaa aggcgttgag cgcgtatgaa tataattctg
     9121 tgggatttgc agcatccttt tccctccttc ggtgaatgcg ctgaaaacgg tttattccag
     9181 ccgtttcagg gtacgcctga taatttgcat tttaaatacc atttattggt tactttttag
     9241 caccatatca gagaagaatc agggaggatt atagatggga agcccgcgac aacgcgggct
     9301 gaaagcatca ggattgcagc gtcgccagtc gggcagcgaa acccacgaac atcaaaccaa
     9361 tcagtgagtt gccaacttta gccagtttct ttttggtacg tatgtactgc gtgacaaaag
     9421 caccagatat aatcaggaag ctcaaatagc agaaactcac cagttccagc gtcgtcgcca
     9481 gaataaagaa tgaaattccc gtatgtgggg cattaacatc gataaactgt acgaaaaacg
     9541 acacatagaa caaaatggct ttcggattag tcaggctcaa aattaacgcg cgtttaaaaa
     9601 tagcaccgta ttggggctca tcggatttgg cctcgctatt tttacccttc agggtcgcgt
     9661 aaagaatttt actccccaga tagagcaaat aaaacgcacc aagataacgt acaatgttga
     9721 ataatatcgg ggtggtctta attaatgtcg ccactccagc ccatgccaga aacatcaata
     9781 ccgcatcgcc aataaataca ccgcaggccg caagatagcc gcctttcata ccgctactga
     9841 cgctattttt gagtacaaac agggtatttg gccctggcac caacacaata aaaatggccc
     9901 caagcagatt aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta
     9961 attatctttc gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag
    10021 aaaggagaaa atcaggtttt aacctgatat caacccgata attgaatcat taatcaggca
    10081 tgctccagtg aaaaattcgg ttgtgctcgg caaaatactg gcgtaaaaat tctaccgtta
    10141 tccgcacttt cgctgacgtc gccagccttg aaacataaac ggcccagacg ttcgctggct
    10201 ggtaatattc cggtagcact tgcactaaat gaccactggc aatgttttcg ctaacatccc
    10261 accaggaacg caaagcaatc ccctgcccat cgagacacca ctgatggaca atttctccat
    10321 gattagacga cagtgggccg gtgactttaa tcgcatggtg gccttcttta ttgcgtaatt
    10381 gccagacacc aaaaggatga tcgcgctctt taatgaccag gcacggcaat gcagaaagat
    10441 cggttaaatg tttcggcgcg ccatgttgcg caataaattc cggcgacgcg cagagaatgc
    10501 ggtaattggt cgccagtttg cgggcgatta aattaggggc gatgtcatcg ccaatgcgaa
    10561 tatcgagatc gacaccttca ttgaccaaat cgaccagccg gtcttccaca tcaaaacgta
    10621 attccagttg cggatacgcc ttcgccagcg ctgataacgc cggagccacc acctgtcgtc
    10681 caaaaccaaa gctgctgata atacgcagca tcccctgtgg cacctgacgc acgtcagaaa
    10741 gttcgtccat catctgaccg acatcctgca aaatccgctg cgcccattca taaatccgct
    10801 ctccttcttc ggtaatggtg acgcggcggg tggtgcggtg taacaacacc acgtttagcg
    10861 tttgctccag caaggcgatg cgcttgctga cgaacgccgg tgaaacgccc agttcttccg
    10921 ccacggcggc aaaaccagcc cggcgagcca ccagcataaa gacgcgcaaa tcattcagca
    10981 gcggtaaatt attcatgatt cgtgttttat gtttcaccag ttacggggat taattccttt
    11041 tcagtcaatt ataggatggt gatgttgtca attttgatgg tcaggaagtg agaacccaat
    11101 aatgaaaacg atgcgtattg ctgcgatccc gggagacggg attggcaaag aagtccttcc
    11161 tgaagggatt cgcgtgttac aggctgccgc tgagcgctgg ggcttcgcct tgagttttga
    11221 gcaaatggag tgggcgagct gcgagtatta cagccatcac ggtaaaatga tgccggacga
    11281 ctggcatgag caacttagcc gtttcgacgc catctatttt ggtgccgtcg gctggccgga
    11341 caccgttccg gaccatattt cgttgtgggg ttcgctgctg aaatttcgtc gtgaattcga
    11401 ccagtacgtc aacctgcgcc cggttcgtct ctttcctggc gttccctgcc cgctggcggg
    11461 aaaacagcct ggcgacatcg atttttacgt ggtcagggaa aacaccgaag gcgaatattc
    11521 ctcgctcggc ggtagagtga atgaaggtac agagcatgaa gtcgtcattc aggaatcggt
    11581 atttacgcgt cgtggtgtcg atcgcatttt gcgttatgcc ttcgaacttg cgcaaagccg
    11641 cccacgtaag acgctaactt ctgccactaa atcaaacggt ttagccatca gcatgccgta
    11701 ctgggatgag cgagtggaag caatggccga gaattacccg gagatccgct gggacaagca
    11761 gcatattgat attctctgcg cgcgttttgt gatgcagccg gaacgcttcg atgtggtggt
    11821 ggcgtccaat ttgtttggcg atatcctttc cgatcttggc ccggcctgca ccggcaccat
    11881 tggcattgcc ccatccgcca acctgaatcc ggaacgcact ttcccgtcgc tcttcgagcc
    11941 tgtccacggt tccgcgccgg atatctacgg gaaaaatatt gctaacccta tcgccacaat
    12001 ttgggccggt gcaatgatgc tcgattttct cggcaatggc gatgagcgtt tccagcaagc
    12061 gcataacggt attctggcag cgattgaaga agtgattgct cacgggccga aaacaccgga
    12121 tatgaaaggc agtgccacca cgccacaggt tgccgacgcg atttgcaaaa ttattttgcg
    12181 ttaaggtcaa accagtttat ttgaaccgcg tcactgacgc ggttttttta ttcgttcttt
    12241 gcagttaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt
    12301 ccgtattctt aattgtttaa tttatgtaac atgcaaataa tcaatttgtt aaacacaaca
    12361 tacaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg gcttttatgc
    12421 ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg ataagcatca
    12481 gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa caatcgaccg
    12541 acgcggccaa cgcaatatac acttttgtta cgcgtacgtt aggttccgca gtacaggtat
    12601 tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac ggcaatattc
    12661 gtcttggcga aggaaaaccg gaatacagca cgctctcctg gctgtttatg tttatttgtg
    12721 ccggtttagg ttcttctacg ctttattggg gggttgctga atgggcctat tattatcaaa
    12781 cgcctggatt aaatatcgca ccgcgttcac aacaggcact cgaatttagc gtcccttact
    12841 ccttcttcca ctggggcatc agcgcctggg caacttatac gctggcctca ttaatcatgg
    12901 cttatcactt tcatgtgcgg aaaaacaaag gtctgagtct ttccggcatt attgccgcga
    12961 ttaccggcgt tcgcccgcaa ggcccatggg gaaaactggt tgatttgatg ttcctgatcg
    13021 ccactgtcgg cgcactgact atttcccttg ttgtcaccgc agcaaccttt actcgtggac
    13081 tttccgcact gaccggttta cccgataact tcaccgtgca ggcatttgtg atcctgcttt
    13141 ccggcggcat tttttgccta agctcatgga ttggtatcaa caacggtttg caacgtctga
    13201 gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt gtcggtccaa
    13261 ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa aacttcctgc
    13321 aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac tggaccgttt
    13381 tctactggct gtggtggatc tcatacaccc ctggcgtagc aatgtttgtc acccgcgttt
    13441 cccgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc accgtcggtt
    13501 gctggttctt cttcggcgta atggaaagct atgccattca tcagtttatc aatggcgtaa
    13561 tcaacgtccc acaggtgctg gaaacactgg gcggcgaaac agctgtacag caagttctga
    13621 tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg attattttcc
    13681 ttgcctcgca tatggatgca gtggcctaca ccatggcggc gaccagtacg cgtaatctcc
    13741 aggaaggtga cgatcctgac cgtgggctgc gtcttttctg gtgcgtggtg atcactctga
    13801 tcccgctttc catcttgttt accggtgctt cgctggaaac gatgaaaacc accgtcgtgc
    13861 tcacagccct tcccttcctc gtcattttac tggtgaaagt cggcggattt attcgctggc
    13921 tgaaacagga ttacgccgac attccggctc atcaagttga acattatctc ccgcagacac
    13981 cggttgaagc cctggaaaaa acaccagtgc tccctgcggg aaccgtattc aaaggcgaca
    14041 actgagcgcc atcatcctaa cgataaaggt atccctatga gcaatctgag ccctgacttt
    14101 gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc tgcccgtttt
    14161 tataccgatc agaacgcctt tgaacacgaa aaagagaacg tcttcgccaa aagctggatt
    14221 tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtaacgcg tgagatcatt
    14281 ggcgaaagca tcgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg
    14341 tgtccgcacc gtggtcatca gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc
    14401 tgcccatatc acgcatgggc attcaaactc gatggcaatc tggcccatgc acgtaactgc
    14461 gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa
    14521 tatgccggat tcgtcttcat caacatggac cccaacgcca ccagcgtaga agatcagtta
    14581 cccggtctgg gcgcgaaagt gctggaagcc tgcccggaag tccacgatct gaaactggcg
    14641 gcccgcttta ccacccgcac gcctgccaac tggaagaaca ttgtcgataa ctatctcgag
    14701 tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt tgatcgttac
    14761 tggcacacca tgcacggtaa ctggacgctg caatacggtt tcgccaaacc gtccgaacag
    14821 tcgtttaaat ttgaagaggg tacggatgcg gcattccacg gtttctggct gtggccgtgc
    14881 acgatgctga acgtcacccc gatcaaaggg atgatgacgg tcatttatga attcccggtg
    14941 gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga gttaaccgac
    15001 gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg
    15061 cttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg
    15121 gccgacagta gcggtagcgg catttccgaa catggtatcg cccatttcca taatctgctg
    15181 gcgcaggtat ttaaggacta atgacatcgg cggcggtatt ttccgccgcc gggctgattt
    15241 ttgatggagt acagcaatgt cagactatca aatgtttgaa gtacaggtga gtcaggtgga
    15301 accacttacc gaacaggtga aacgcttcac gctggtggca accgatggca aaccgctgcc
    15361 tgcgttcacc ggaggaagtc acatcatagt gcagatgagc gatggtgata accagtacag
    15421 caatgcgtat tcactactga gttcaccgca taacacctct agttatcaga ttgccgttcg
    15481 actggaagaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa aagtgggcga
    15541 tcggttaacg atctcaacgc ctaataacct gtttgcgcta attccctcag ccagaaagca
    15601 tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcgcacatgg cagagttgca
    15661 acacagcaac gtcgactggc agctacatta ctgctcgcga aatccagaaa gttgcgcatt
    15721 tcgtgatgag ctagtccagc atccgcaggc tgagaaagtc catttgcatc attcatcaac
    15781 cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca cacacgttta
    15841 tacctgtggc cccgaggcgc taattgaagc ggtaagaagt gaagctgcgc gtctggacat
    15901 cgccgccgat acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcatt
    15961 taccctagtg cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga tgactatttt
    16021 gcaggttatt gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg aagggatatg
    16081 cggaacctgc gaaacggcaa tactggaagg tgaagctgac catcgggatc agtattttag
    16141 cgatgaagag cgtgccagcc agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa
    16201 acgcctggtg ttggatttgt agtttgccga agccggatgt ggcgctgagc gcgcccagtc
    16261 cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat
    16321 cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca
    16381 gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatatt
    16441 atgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc
    16501 cggcatgtcc atcaggttaa gcatcggctg cggtaaggca tcttccggca atgtctgcgc
    16561 tttttccacc agcgccagca gcgttttacc gtgaaagcgg atttcgctac cggataaacc
    16621 caggctgtcc agttcgccta aacttcccgg catataacgc gctaccgacc acaaatgctc
    16681 ttcacgcacg acaaagttca ccgccagatc gcgctctcgc gccttgcgca gtcgccagtc
    16741 ggctaacagt tgcagacagg ccagttggcg tgtgcgtaat tgccaggcat tggtgatatc
    16801 acgccaggca tcttccggcg caacgacttc ctgacgacgc atttgcatca ggcgacattc
    16861 atccagcgcc gctggtagcc agccggaggc ctccgtttct accataagct tggcggtgat
    16921 cggtaacaga taccagacat ccgccgctgc atattcacac tgacgttcgg tcagcggtct
    16981 ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac
    17041 catggaagcg aaaccccatg acatcgggcg tccgcagaag gcagcaagga tttgcgtgtc
    17101 aatcaggggt tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc
    17161 tgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc
    17221 ggtgatcccg agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc
    17281 cagctgcggg taataagtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa
    17341 ggcacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt
    17401 caaattgtat tctctttagt gggcgtcaaa aaaaaagccg gattaaccgg cgtctgacga
    17461 ctgacttaac gctcaggctt tattgtccac tttgccgcgc gcttcgtcac gtaattctcg
    17521 tcgcaaaatt tttccgacgt tagatttcgg taactcatca cgaaactcca ccagcttcgg
    17581 tactttgtag cccgtgagct gacggcggca aaaggtcacc agtgactctt cggtaagcga
    17641 tggatctttt ttcactacga agattttcac cgcttcacca ctggagccgg aaggtacgcc
    17701 aacagccgcg acttcctgta cgccaggatg ctgcatgacg acatcttcaa tctcgttggg
    17761 atagacgtta aaaccggaaa ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa
    17821 cccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt tgatgatctc
    17881 atctgtagca tccggacgct gccagtaacc cagcatcacc tgcggtcctt tgacacaaag
    17941 ctcacccggt tgacccggtg gtacttcatt atcatcatca tccaccagtt tggcttccgt
    18001 cgacggcacc ggcaaaccga tgctaccact atgataatca atatcatatg ggttaacgct
    18061 gaccagcggc gcacactcgg taaggccata gccttccagc agatactgtc ctgtcagttt
    18121 cacccaacgc tctgccacca cttgctgcac tggcatccca ccgcctgcgg aaagatgcag
    18181 actggagaaa tccagctgct ggaactcttt attgttcagc aacgcattga acaaggtgtt
    18241 aacgccagtg atagcggtaa acggatattt cgctaactct tttaccaacc ctggaatatc
    18301 acgcgggtta gtgataagca ggttctgccc acccagttcg ataaacagca ggcagttaat
    18361 ggtcagggca aaaatgtgat acagcggcag tgccgttact accagttctt tacctggatg
    18421 cagcaacggc ccataggtag cgttgacctg ttccaggttc gccagcatat tgcggtgagt
    18481 cagcatcgcg cctttcgcca cacctgtggt gccgccggtg tattgcagga aggctaaatc
    18541 ttccggcacc agttcgggtt tgacgtactg catccggtag ccgttatgca gtgcgctacg
    18601 aaatgaaatg gcatctggca gatggtattt cggcaccaaa cgcttgatgt atttaacaac
    18661 gaaattgact accgtgcctt ttgccgtaga tagctgatcg cccatacggg tcagaattac
    18721 gtgctgaacg gcggttttat caactacctt ttccagagtg tgggcaaagt tagacacgat
    18781 aacaatcgcc gatgcgccac tatcgttaag ctgatgctca agctcgcgcg gggtatacaa
    18841 cgggttgacg tttacgacaa tcatcccggc gcgcaaaatg ccaaacagcg ccactggata
    18901 ttgcaataag ttaggcatca tcagcgcaac gcgatcgcct ttcttcagcc ccaacccttg
    18961 ttgcaaataa gcggcaaacg cgcgactgcg ctcttccagc ttgcggaagg tcatcacctc
    19021 ccccatattc acaaacgcag gctgatcggc gtagcgcgcg accgactgct caaacatatc
    19081 taccagagat tgataacggt cagggttgat ctccgtcgga acgtccgcgg gataacggtt
    19141 aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc
    19201 aaatgatata catgccgtta acataatatt aactcatcat accagcttga taattaccca
    19261 acgaaaaggt tgcgaagcgc gtcactattt atttttatct ttaccgtaag aatgcagaaa
    19321 cagcggacca gccgctgttt ctttttcttt aaaaacaagc aattattcag ttacaactgt
    19381 ttgtactctc gcggggccgg gattatacca gccccatccg ccgtagccat agggccagcc
    19441 acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc ataatcacct gctgggtaaa
    19501 atgccaacgt ttgtaacccg ttacttgcat caccataaat ttatagggcg tattgccgat
    19561 tttgccgtca accgcaccag tgattggccc gactaccgta accagttgtc cacggaaatc
    19621 caccgggtcc agaaaaccgt tcacatcggc ataaatgcga ccgcgagaag gttctcccag
    19681 cgtcggtctg gctccgctgt ccagcggtac ggtagcaatc tccaggcggg ttttcccttg
    19741 ctggttttgt accgcaacca ctttgccacc aaagcgtgcc tcctgaccaa cgtacagctg
    19801 cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg ggactgctgc ctttaatggc
    19861 gtccggcaca gtgacacaac cgctcagcat tagcgcaaac gtacctgcca gtatgccttt
    19921 gataacattc ttttgaaccg ccatgatgcg actccttttt ttctcagggc atactcttaa
    19981 gattcattct ttgcccggaa gtttcttcca tgcgacgttg ttacgtaaat aaaccggttc
    20041 ggcatgttcc accgccaccg ttttaccctc ggcaaacatc tgacacgcaa tcggcagcat
    20101 atcttccgcc gctggcagta aaacttctcc gtcgcgcaaa accagcccgc tctctttacc
    20161 gagatccggc caggcttgcc agcccgtccc taccgtcacc cattcgccgg aaagctgttg
    20221 cattcgttca tgaacgagtt cgggtttgag tacggcttcg gtttcttcac cgtgccagat
    20281 accgttttca tcacgctgat attcggccca gtaaacttcg cccattcgcg cgtcaatggc
    20341 tgacagcacg cgtgttgcgc cgtttttgcg ccacgcccct tgcgccatcg tcattagcgt
    20401 ggagacgcca atcatcggta attccgcgcc aagcgccagc ccttgcgcga tgccaatacc
    20461 aatgcgcacg ccggtaaagc taccggggcc gcgcccgtaa gccagagcgt taatatcagt
    20521 cagggaagtt ccgctggtgg tcaggatatc ctgcaccatc ggtaagattc gttgagtatg
    20581 ttcacgaggg caaagctcaa aatgagcgtt gacagtaccg tcgttccaca gggcgacaga
    20641 gcaggcctct gtcgcggtat cgatagccag aattcgcatg ggtcttcgtg cttagatcaa
    20701 taaaaaggcg cgcatcatac catactccgt aacaaattac ctggaggatg gtatcgcaag
    20761 gaaacgaacc gcacgggcaa tgtcacgggt gcgcggcgct ggcggcagac tggcgagaaa
    20821 cgtcgcgccg taagggcgca tcaccagccg gttgtcgcaa atcaccaaaa ccccgcgatc
    20881 gtcggcgtcg cgaatcagtc gccctacccc ctgcttgaga gtaatgaccg catccggcag
    20941 ttgcacttca tcgaacgggt cgccaccgcg caaacgacaa tcttccatgc gcgcttttaa
    21001 cagtggatca tccggcgagg taaacggcaa tttgtcgata attaccaatg acaatgtatc
    21061 gccacgcacg tccacccctt cccagaaact actggttgcc acaagaagcg cgttaccggc
    21121 gctgacaaat tgctgcaaca gttgcccttt gctggtttcc ccctgcaaca aaacgggaag
    21181 cgtcatggta gcgcggaact gctcggccag atcgcgcatc atggcgtgcg aggtacaaag
    21241 cataaaacaa cgaccgttgt tagcttcgat gatcggtcgc agcattgccg ccagttgccg
    21301 tgcacaaccc ggttgattgg tttgcggcag gttgcgcggc acacagagta acgcctggcg
    21361 gctgtaatca aacgggcttg gcaatagcaa cgactccgcc tgttctatcc caagccgcga
    21421 ggtgaaatga tgcagatcgt cgttcaccga cagcgttgct gaggtgaaga tccagctacc
    21481 gggcttttgc gccattaact ctttgaattt atccgccacg ctgagcggcg tgagagccag
    21541 agtaaaatgg cgcgaagtgc attcgtacca gtagctgtag cccggctgat tgatctcttt
    21601 tagccgcttc agccgtgtgc gatacaacgt ggcgcgctca aatgccgcat ccagcaaggc
    21661 ggaacgcccc agtgacagtt tcgccacgtc ataacaaagt tccagggtgt catcgagcag
    21721 taaaaatgcc cgctgaattt gcggattagc taacagctca cgcaggttac cgcgataacc
    21781 tggctcaccg agttgcagac gaaaatcctg cgcactctgg gcaagacgat cagcgcactt
    21841 ttgtaactgc tgggtgtctt ttaattcggt gcggtaggcg atggtgatgt cttttgccag
    21901 gtcgagcagt tgtcgactgg agagtgactg accaaaatac tggctggcaa tgtccggtag
    21961 ctggtgggct tcgtcgaaga tcatgacgtc cgcttccggg atcagttcgc caaatcgact
    22021 ctctttaacc accatatccg ccagaaagag atgatggttt accaccacca catcggcgtc
    22081 catcgctttt ttacgtgctt tgaccacaaa gcaatcttta tacatcgggc agtcgctgcc
    22141 aagacagttg tcgttggtgc tggtgaccag cggccacgcc tgtgaatctt ccgccacgct
    22201 gacgcaggtg ctgatatcac catcgactgt ttgattagac caggagcgca gcaggatcac
    22261 atcgcttaag atttgtaccg gcagttcgcc ccccgccagc gcctgctgtt cgagacgctc
    22321 gaggcagagg tagtttgagc gccctttcag cagcgccacg ttgcccgtat atttcaatgc
    22381 ctttgagact gttggcaaat cgcggctgta gagctgatcc tgcaacgctt ttgagccggt
    22441 cgagataatg actttctttt tcgcccgcag cgcaggagcc aggtaagcgt aggttttgcc
    22501 cgtaccggtt cctgcttcca ccaccagcgg ctggcctttt tctatcgcct gggtgacggc
    22561 taccgccatc tgtcgctgtg gttctcgcgg cttaaagcct ggtatcgctt tcgccagctg
    22621 accgtctggt gcaaaatcgt ccgtcacact accccctgtt gatttgaaca gggattatgt
    22681 caggatgagg gcgctttcgc cagttgaagt ggtgacggcg acctcacatt gtggcagtct
    22741 ttgcagcacg aaaatggaag gataatgagg acaaaatgat gactatcgtt cgtatcgatg
    22801 ctgaagcccg ctggtctgat gtagtaatcc acaacaacac gctctactac actggtgtac
    22861 cggaaaacct cgacgccgat gcctttgagc aaaccgccaa cacgctggcg cagattgacg
    22921 ccgtgctgga aaaacagggc agcaataaat cgagcattct ggatgccacc attttcctgg
    22981 ccgataaaaa cgacttcgcg gcgatgaata aagcgtggga tgcctgggtt gtcgcgggtc
    23041 atgcgccggt gcgctgcacg gtacaagcgg gtttgatgaa cccgaagtat aaagttgaaa
    23101 ttaagattgt ggctgcggtg taagctttat cgaagcaaaa taagtcagac gataatttat
    23161 cgataatact ggtcggtttt acataaatcg accagagaat
//
LOCUS       DAZUGK010000061        23050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZUGK010000061 DAZUGK010000000
VERSION     DAZUGK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.061"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..719
                     /gene="ruvA"
                     /locus_tag="ACHY5W_002038"
     CDS             108..719
                     /gene="ruvA"
                     /locus_tag="ACHY5W_002038"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4446934.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            728..1738
                     /gene="ruvB"
                     /locus_tag="ACHY5W_002039"
     CDS             728..1738
                     /gene="ruvB"
                     /locus_tag="ACHY5W_002039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4446935.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ACHY5W_002040"
     CDS             complement(1984..2769)
                     /gene="znuB"
                     /locus_tag="ACHY5W_002040"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFS4446936.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ACHY5W_002041"
     CDS             complement(2766..3521)
                     /gene="znuC"
                     /locus_tag="ACHY5W_002041"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFS4446937.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3600..4532
                     /gene="znuA"
                     /locus_tag="ACHY5W_002042"
     CDS             3600..4532
                     /gene="znuA"
                     /locus_tag="ACHY5W_002042"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFS4446938.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4548..5870
                     /gene="mepM"
                     /locus_tag="ACHY5W_002043"
     CDS             4548..5870
                     /gene="mepM"
                     /locus_tag="ACHY5W_002043"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFS4446939.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5990..6961
                     /gene="lpxM"
                     /locus_tag="ACHY5W_002044"
                     /gene_synonym="msbB"
     CDS             5990..6961
                     /gene="lpxM"
                     /locus_tag="ACHY5W_002044"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFS4446940.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ACHY5W_002045"
     CDS             complement(7092..8534)
                     /gene="pyk"
                     /locus_tag="ACHY5W_002045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4446941.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ACHY5W_002046"
                     /gene_synonym="yebK"
     CDS             complement(8662..9531)
                     /gene="hexR"
                     /locus_tag="ACHY5W_002046"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFS4446942.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9869..11344
                     /gene="zwf"
                     /locus_tag="ACHY5W_002047"
     CDS             9869..11344
                     /gene="zwf"
                     /locus_tag="ACHY5W_002047"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4446943.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11579..13390
                     /gene="edd"
                     /locus_tag="ACHY5W_002048"
     CDS             11579..13390
                     /gene="edd"
                     /locus_tag="ACHY5W_002048"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFS4446944.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13427..14068
                     /gene="kdgA"
                     /locus_tag="ACHY5W_002049"
                     /gene_synonym="eda"
     CDS             13427..14068
                     /gene="kdgA"
                     /locus_tag="ACHY5W_002049"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4446945.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ACHY5W_002050"
     CDS             complement(14124..15302)
                     /gene="purT"
                     /locus_tag="ACHY5W_002050"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFS4446946.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15436..15726
                     /gene="yebG"
                     /locus_tag="ACHY5W_002051"
     CDS             15436..15726
                     /gene="yebG"
                     /locus_tag="ACHY5W_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFS4446947.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15793..16149
                     /gene="yebF"
                     /locus_tag="ACHY5W_002052"
     CDS             15793..16149
                     /gene="yebF"
                     /locus_tag="ACHY5W_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFS4446948.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16476..17135
                     /locus_tag="ACHY5W_002053"
     CDS             16476..17135
                     /locus_tag="ACHY5W_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS4446949.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17344..19404
                     /gene="ptrB"
                     /locus_tag="ACHY5W_002054"
     CDS             17344..19404
                     /gene="ptrB"
                     /locus_tag="ACHY5W_002054"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFS4446950.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ACHY5W_002055"
     CDS             complement(19401..20063)
                     /gene="exoX"
                     /locus_tag="ACHY5W_002055"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFS4446951.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTIFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ACHY5W_002056"
     CDS             complement(20087..20743)
                     /gene="yobB"
                     /locus_tag="ACHY5W_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFS4446952.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ACHY5W_002057"
     CDS             complement(20845..21075)
                     /gene="holE"
                     /locus_tag="ACHY5W_002057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFS4446953.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21214..21588
                     /gene="yobA"
                     /locus_tag="ACHY5W_002058"
     CDS             21214..21588
                     /gene="yobA"
                     /locus_tag="ACHY5W_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFS4446954.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLMHQYPAANAQVTAS
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21592..22464
                     /gene="copD"
                     /locus_tag="ACHY5W_002059"
     CDS             21592..22464
                     /gene="copD"
                     /locus_tag="ACHY5W_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFS4446955.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            22477..22818
                     /locus_tag="ACHY5W_002060"
     CDS             22477..22818
                     /locus_tag="ACHY5W_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFS4446956.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKTQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT         5805 a         5731 c         5974 g         5540 t
ORIGIN      
        1 cagttcatcg agacacctcg caagttttct tcatccttcg ctggatatct atccagcatt
       61 tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac
      121 tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag
      181 gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag
      241 cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca
      301 ataaacaaga gcgcacattg tttaaagagt tgatcaaaac caacggcgtc ggtccgaagt
      361 tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg
      421 aagaagtggg ggcactggtg aaactgccgg gtattggaaa aaaaaccgcc gaacgcttga
      481 ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg ccagccgccg
      541 acctggtact cacgtctcct gccagcccgg cgaccgacga tgctgaacaa gaagcggttg
      601 ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg
      661 ctcgccctga cgctagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag
      721 gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt
      781 agcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg
      841 ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt
      901 gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga
      961 aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc
     1021 tgcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgttt
     1081 atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat
     1141 gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat
     1201 tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt
     1261 gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagcgcacg
     1321 ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg
     1381 tacgccgcgc attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca
     1441 cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga atgtcgatgc
     1501 tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg
     1561 tgggccggta ggtctggata acttggcggc agccattggc gaagaacgtg aaaccattga
     1621 ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgtgggcg
     1681 tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt
     1741 cggattggtc gctcgcgccg catccgacat tggcagtgtg cctgatgcga cgctattcgc
     1801 gtcttatcag gcatatacca gagatagcga accgtaggtc ggaaaaggcg ctcgcgccgc
     1861 atccgacatt ggcagtgtgc ctgatgcgac gctgttcgcg tcttaccagg cctaccagag
     1921 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     1981 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     2041 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     2101 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     2161 tgcagtagct gcaggaataa tcagcagcga agtaataatc aacgcgccga caaatttcat
     2221 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactc gctgtaattt
     2281 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     2341 ccagaacaaa atagccacca cgatgaccac gccaattgca atagagatga gatcttctgg
     2401 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     2461 cagactaacg accaccaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     2521 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     2581 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     2641 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     2701 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     2761 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     2821 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     2881 ataaactccg gatgcaggga aacaacctcc ggtgtgccgg aacaacaaat gtggtgattc
     2941 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     3001 acgccacaat ccagttcacg acgcagttgg tcaataaggt catataacgc cacctggcca
     3061 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggcg atttaacaat
     3121 gctcgcgcta acagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     3181 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     3241 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     3301 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     3361 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     3421 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     3481 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     3541 tagtcatgaa atgttataat atcacacttt tcatattcat tacgatgatt ggtcgcatta
     3601 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     3661 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     3721 ccattgctga tggggtaaca gaaacggagg ttttacttcc tgacggcgct tcagaacatg
     3781 attattcact ccgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     3841 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     3901 aggtaacgat tgcgcagcta gaagatgtga aaccgctact gatgaaaagt attcacggcg
     3961 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     4021 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     4081 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     4141 aggcacaatt agcttcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     4201 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     4261 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     4321 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     4381 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     4441 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     4501 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     4561 cccgctctgt cgccctggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     4621 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgcgatg
     4681 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     4741 aagccagtga accgattgat caagctgcac aagaagatga agccattccc caggacgaac
     4801 tggatgacaa aattgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     4861 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caactggctg
     4921 cggccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     4981 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     5041 accgtactgc tgctaacggt tttaaaatga ccagcgaaat gcagcaggga gagtgggtta
     5101 acaatctgct gaaaggtacc gtcgggggga gctttgttgc cagcgccaga aacgccggtt
     5161 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
     5221 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
     5281 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcta
     5341 tccgcgctga agatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
     5401 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgtacta
     5461 atccggtgac cggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccgcagggta
     5521 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
     5581 gttattatgt agctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
     5641 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
     5701 ataccggacg ttcaaccggg ccgcatctgc actatgaagt atggataaac cagcaggccg
     5761 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
     5821 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
     5881 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgcgcc
     5941 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
     6001 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
     6061 gggagcatgg ctgggcgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
     6121 tgaccccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
     6181 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
     6241 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
     6301 aatacgcggg ccggagaaaa ttcagccgcg cgttgactgg caagggctgg agatcatcga
     6361 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
     6421 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
     6481 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
     6541 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
     6601 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
     6661 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtgtgcc gtgcgcgcgt
     6721 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
     6781 accgatggat gatctgttag aggcggatga tcatacgatt gcgcggcgga tgaatgaaga
     6841 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
     6901 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
     6961 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
     7021 attcgaaatc tgttgaacta tcattgaact gtaggccgga tgtggcgttt tcgccgcatc
     7081 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
     7141 atcacgtcgc cctgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
     7201 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
     7261 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
     7321 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
     7381 atggtaatga tcgccgtaac gcctttcagg tggttagctg cgtacattgc tgacatggca
     7441 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
     7501 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
     7561 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
     7621 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
     7681 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
     7741 atgccgacca gttccgggtc gccaatttct acaccgaggt cgccacgtgc aaccattacc
     7801 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
     7861 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
     7921 ttcagatctt cgccgcagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
     7981 gcagttttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
     8041 agtttgttga tacctttatt gttggagagg ggaccaccaa cggtgacttc ggtgaacact
     8101 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
     8161 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
     8221 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
     8281 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
     8341 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
     8401 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
     8461 tcaaggttat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt agttctgcga
     8521 agccttctgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
     8581 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
     8641 ttagaacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
     8701 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
     8761 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
     8821 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
     8881 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atagcgtcgt tttcgcgtgc
     8941 cagctgcgcc agctcgacca gattttttgt tcttccggtg tgagaaatca gcaccaccac
     9001 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acaatatcat cggagtacac
     9061 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggctgaaga
     9121 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
     9181 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
     9241 tttccctgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
     9301 gccattcgcc agactctgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
     9361 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
     9421 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
     9481 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
     9541 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
     9601 attacgcctg tgtgctgtgt taatgacaaa agcagataaa aaagttgtta ttttttttca
     9661 taacatgatc agtgtcagat ttttacccaa tggaaaacga tgattttttt atcagttttg
     9721 ccgcactttg cgcgctttta ccgtaatcgc acgggtggat aagcgtttac agttttcgca
     9781 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
     9841 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
     9901 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
     9961 tcaactggaa aaagccggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    10021 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    10081 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    10141 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    10201 tatcaccatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    10261 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    10321 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    10381 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    10441 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    10501 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    10561 gatgcgcgac atgatccaga accacctgct gcaaattctt tgcatgattg cgatgtctcc
    10621 gccgtctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtctct
    10681 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    10741 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaagagcag
    10801 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    10861 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    10921 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    10981 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    11041 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    11101 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    11161 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    11221 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    11281 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    11341 gtaatatctg cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    11401 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    11461 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    11521 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    11581 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    11641 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    11701 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag cctctttgaa
    11761 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgctca
    11821 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    11881 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    11941 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tcgggctgtc
    12001 ccataacatg tttgatggtg ctctgttcct cggggtgtgc gacaagattg tcccgggtct
    12061 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    12121 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    12181 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    12241 tactttctac ggtactgcca acaccaacca gatggtggtg gagtttatgg ggatgcagtt
    12301 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    12361 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    12421 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    12481 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    12541 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaatggtcc
    12601 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    12661 caaagctggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    12721 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    12781 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    12841 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    12901 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    12961 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    13021 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    13081 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    13141 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    13201 ggacatcatt cgtgtgaatg gacagacagg cgaactgacg ctgctggtag acgaagcgga
    13261 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    13321 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    13381 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    13441 aacaagtgca gaatcaatcc tcaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    13501 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    13561 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    13621 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    13681 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    13741 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    13801 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    13861 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    13921 tccggctaac taccgtgact acctggcgct gaaaagcgtg ctgtgcatcg gtggttcctg
    13981 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    14041 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatcgagg atcgggcatt
    14101 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    14161 gcgcgttcaa tggcgtcaac aacactttct gcagtagcca gtgccacccc cagacgacgg
    14221 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    14281 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    14341 gcaggaccat actgacggat cccgccaacc ggaagtccga ggaaggcacg tacatgcagg
    14401 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    14461 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    14521 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    14581 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    14641 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    14701 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    14761 gcgtattccc atgcctgagc aagttgctct gcagaacgaa taaacgtctg ccccttgccg
    14821 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    14881 aaaaggcttt cgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    14941 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    15001 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    15061 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    15121 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc gtagcgatcg
    15181 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    15241 ccggagccta ataacatcac acgagttgct gccggacgca gcgcagtgcc taataacgtc
    15301 ataacgaata tcccctgata aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    15361 tttattgctg gtgttgattt ctcaaccgaa aagaaataca ctgtataaaa tcacagttat
    15421 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    15481 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    15541 gatctgctgg acacctggct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    15601 gccctttcgc tatggctggc agaacaaaaa gatgtgctga gcaccattct gaaaaccggc
    15661 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    15721 gcctgaagca acttgcactc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    15781 atggagaaaa acatgaaaaa aagaggggcg tttttagggc tgttgttggt ttctgcctgc
    15841 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg taactttccc aaagtgtgaa
    15901 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    15961 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc atgggtcagt
    16021 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    16081 agtgccgata ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag
    16141 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    16201 tccgccccaa aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    16261 aatcagcatc aaacaatgcc actcaaaacg tcaaactact ttcacctaaa atacccttat
    16321 cacctgatat acagtaaaca ccttacctgg cgcgaatttc tttgaataac ggccaaccgc
    16381 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccactatg
    16441 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    16501 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    16561 aacttttagt gccgggcgca ttaggtggac tggctggact gctggtcgca aataaatcag
    16621 cacgtaaact tcttacgaaa tatggcacca acgcgttgct ggttggcggc ggagcggtag
    16681 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    16741 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    16801 tagtctttgc cgctaaaagt gatggtcata ttgatgccaa agaacgtgcg gcaatcgacc
    16861 agcaattgcg tgaagccggc gtggaagagc aggggcgggt actcattgag caggcaatcg
    16921 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    16981 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    17041 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    17101 atctcgaaca gcaaaaacgc actttggcgg aataatcccg ctctctggat agcctgaagt
    17161 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    17221 gcataacatt cgatattcgc gccattacct gtcatcatct aagcaatgac tgccctgttt
    17281 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttcaaccag aaagaacaat
    17341 aacatgctac caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc
    17401 atcgataatt actactggct gcgggacgat acgcgttctc agccggaagt cctggactac
    17461 ctgcaacaag aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat
    17521 cgcatcttaa aggaaatcat cgaccgcatt ccgcaacgag aagtttctgc gccctacatc
    17581 aaaaatggct accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag
    17641 cgtcaatcgg cgttcagtga agagtgggac gagtgggaaa tattgctcga tgccaacaag
    17701 cgcgcggctc atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc
    17761 attatggcgc tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat
    17821 ctggaaacag gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg
    17881 gcgaatgact cctggacttt ctactatgtt cgcaagcatc cagtgacgct gctgccttat
    17941 caggtctggc gtcacgctat cggtacgcca gcatcgcaag ataaactgat ctacgaagaa
    18001 aaagacgata cctattacgt cagcctgcat aaaacgacgt cgaagcacta tgtagtcatt
    18061 catctggcca gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc
    18121 gagccgtttg tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag
    18181 catcgttttt atctgcggtc caaccgccac ggcaaaaact ttggcttata ccgtacccgt
    18241 atgcgtgatg agcaacagtg ggaagagtta attccgccac gcgataacat tatgctggaa
    18301 gggtttacgc tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt
    18361 ttgcgccaaa ttaaccgcaa gacccgggaa gtcattggta ttgcctttga tgatccggcc
    18421 tatgtgacct ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat
    18481 tcttccatga ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt
    18541 gtattaaaac aaacggaagt tcctggtttt gatgcggcga attaccgcag tgaacacctg
    18601 tggatagtcg cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat
    18661 tttcgcaaag gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt
    18721 gatgccgatt tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt
    18781 gtccatgttc gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg
    18841 aagaagaaaa atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc
    18901 tatggctctc cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc
    18961 gttgcaatta atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt
    19021 gatgttgtaa caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag
    19081 tgggggaatc cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac
    19141 aacgtcaccg cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag
    19201 gtgcaatatt gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgataac
    19261 catcttttat tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt
    19321 aaatcgtacg aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca
    19381 ttacccgcta cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc
    19441 tccgggctca tgctgtccag gttattaaat aaccagcgca gatagcccgg atcgcgttcg
    19501 gcaacgtcgg aaaccgcttt gccacggtat ttaccaaagg tgaagatcgt catcaacgac
    19561 ggacgtccgg tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg
    19621 ataagcaacg cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc
    19681 ggcgtctgta cattgagctt gcgtgtttta tataacgcca tattgctgta cttgatccct
    19741 ggccacaaac gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt
    19801 acgcggcggt caaagctggc gttatgcgcg acataccatt cactaccgta atagtgtggg
    19861 atcacatctt caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc
    19921 atcgcttgtg gactaatagg acgatcgggg cgcaccaggt ggctcatggg gttgacgatt
    19981 tttccgtcaa tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt
    20041 tctgtatcga taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa
    20101 tgatatcgcc ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat
    20161 cagcgcgaac gatgagacga ccatgttcat cccatagtgc actgttacca cgggcatttg
    20221 ccatcagcac agcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca
    20281 gcaggtcggg ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat
    20341 ccataccttg cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg
    20401 caccatgact ctgatggtag atcccaggcg tttttcgcca cggtgcgaac accgcaattc
    20461 cacgaataaa gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgccagg
    20521 tcgttgcggc ataacaaagc gggtctaaca gtgagagatc gggtggggca ggaagggcac
    20581 gtcgcgaata atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt
    20641 gcctggcggc ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt
    20701 tacgaggctc atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa
    20761 aatgactgca aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta
    20821 agaaagtgta actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag
    20881 attgaccgaa gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc
    20941 aggctgttca cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaatgc
    21001 caccccggcc gccgccaaat cgacattcac tttatccatt tctgtttgat ccagttttgc
    21061 cagattcttc agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag
    21121 cctggatggc aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct
    21181 gtaaaaaagt tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc
    21241 tacgcgctgg caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat
    21301 ttaatgcatc agtatcctgc ggcaaacgcg caagtgacag catctccgca ggcaatcact
    21361 ttaaacttct cggaaggtgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa
    21421 aacgaaaata ttaaaacatt accggcgaag cgaaatgagc aggaccaaaa gcaactgatt
    21481 gttccactgg ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg
    21541 gtggacgggc ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg
    21601 tttacctgga tcgcgctgcg atttatccat ttcacctcgc tgatgctggt ctttggcttc
    21661 gcaatgtacg gtgcctggct ggcaccctta acgattcgtc gcttgctgac gaagcgtttt
    21721 ctgcgtttac aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc
    21781 gttcagggcg ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag
    21841 gctgttttgc aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta
    21901 acgttgatcg tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact
    21961 accgcgcaat ttattctgct ggcgggagtc ggccatgcga cgctgaatga aggggtaacg
    22021 gcgaaaatcc atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg
    22081 ggattactgc cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acaccaggct
    22141 attcaggcgc tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca
    22201 agcggcgtgc ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg
    22261 ggccaactct tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct
    22321 aatcgttatg ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt
    22381 gtgtggatga ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg
    22441 cttgcgactc tcgaaccttt ttgatggatt atgaaaatga tgaaaaaaag tattctggcg
    22501 tttctgttac tcaccagttc tgcagcggcg ctggctgcac cgcaggtgat taccgtcagc
    22561 cgttttgaag tgggtaaaga caaatgggcg tttaatcgcg aagaggtgat gctgacttgc
    22621 cgaccgggta atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac
    22681 gatatcgcac aaaaggaagt tgccagtggg aagactaaaa cccaacccat ttcggtgatt
    22741 cagattgatg atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga
    22801 gctgaaaaac tctgttaatt acctaaaata gccttttgat ttccaataaa aaaccgcctc
    22861 agttctttca ccagaacggg cggtttttaa catttaagct gatgaccacc gcgcttttta
    22921 ttgaccattt tgcacgcaaa ctggaaaacc tggcgtcgtc atctattctt aaagggcaag
    22981 gcaactaagc ctgcattaat gccaactttt agcgcacggc tctctcccaa gagccatttc
    23041 cctggaccga
//
LOCUS       DAZUGK010000062        22703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZUGK010000062 DAZUGK010000000
VERSION     DAZUGK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.062"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(502..1593)
                     /gene="ychF"
                     /locus_tag="ACHY5W_002061"
     CDS             complement(502..1593)
                     /gene="ychF"
                     /locus_tag="ACHY5W_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4446957.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1710..2294)
                     /gene="pth"
                     /locus_tag="ACHY5W_002062"
     CDS             complement(1710..2294)
                     /gene="pth"
                     /locus_tag="ACHY5W_002062"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4446958.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPISEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2572..2850
                     /gene="ychH"
                     /locus_tag="ACHY5W_002063"
     CDS             2572..2850
                     /gene="ychH"
                     /locus_tag="ACHY5W_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFS4446959.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2905..4584)
                     /gene="dauA"
                     /locus_tag="ACHY5W_002064"
     CDS             complement(2905..4584)
                     /gene="dauA"
                     /locus_tag="ACHY5W_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFS4446960.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVVGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4709..5656)
                     /gene="prs"
                     /locus_tag="ACHY5W_002065"
     CDS             complement(4709..5656)
                     /gene="prs"
                     /locus_tag="ACHY5W_002065"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4446961.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5807..6658)
                     /gene="ispE"
                     /locus_tag="ACHY5W_002066"
     CDS             complement(5807..6658)
                     /gene="ispE"
                     /locus_tag="ACHY5W_002066"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4446962.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVAPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6658..7281)
                     /gene="lolB"
                     /locus_tag="ACHY5W_002067"
     CDS             complement(6658..7281)
                     /gene="lolB"
                     /locus_tag="ACHY5W_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFS4446963.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7495..8751
                     /gene="hemA"
                     /locus_tag="ACHY5W_002068"
     CDS             7495..8751
                     /gene="hemA"
                     /locus_tag="ACHY5W_002068"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFS4446964.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8793..9875
                     /gene="prfA"
                     /locus_tag="ACHY5W_002069"
     CDS             8793..9875
                     /gene="prfA"
                     /locus_tag="ACHY5W_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4446965.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9875..10708
                     /gene="prmC"
                     /locus_tag="ACHY5W_002070"
     CDS             9875..10708
                     /gene="prmC"
                     /locus_tag="ACHY5W_002070"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4446966.1"
                     /translation="MEYQYWLREAINQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10705..11097
                     /gene="sirB2"
                     /locus_tag="ACHY5W_002071"
     CDS             10705..11097
                     /gene="sirB2"
                     /locus_tag="ACHY5W_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFS4446967.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11101..11910
                     /gene="sirB1"
                     /locus_tag="ACHY5W_002072"
     CDS             11101..11910
                     /gene="sirB1"
                     /locus_tag="ACHY5W_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFS4446968.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11946..12800
                     /gene="kdsA"
                     /locus_tag="ACHY5W_002073"
     CDS             11946..12800
                     /gene="kdsA"
                     /locus_tag="ACHY5W_002073"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFS4446969.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKALDDLVKG
                     FEELDTSK"
     gene            complement(12948..13055)
                     /locus_tag="ACHY5W_002074"
     CDS             complement(12948..13055)
                     /locus_tag="ACHY5W_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4446970.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13484..13591)
                     /locus_tag="ACHY5W_002075"
     CDS             complement(13484..13591)
                     /locus_tag="ACHY5W_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS4446971.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVDWWRNRK"
     gene            complement(13996..15096)
                     /gene="chaA"
                     /locus_tag="ACHY5W_002076"
     CDS             complement(13996..15096)
                     /gene="chaA"
                     /locus_tag="ACHY5W_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFS4446972.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15366..15596
                     /gene="chaB"
                     /locus_tag="ACHY5W_002077"
     CDS             15366..15596
                     /gene="chaB"
                     /locus_tag="ACHY5W_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFS4446973.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKKDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15754..16449
                     /gene="chaC"
                     /locus_tag="ACHY5W_002078"
     CDS             15754..16449
                     /gene="chaC"
                     /locus_tag="ACHY5W_002078"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFS4446974.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRIVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16493..16846)
                     /gene="ychN"
                     /locus_tag="ACHY5W_002079"
     CDS             complement(16493..16846)
                     /gene="ychN"
                     /locus_tag="ACHY5W_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFS4446975.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17032..18426
                     /gene="ychO"
                     /locus_tag="ACHY5W_002080"
                     /gene_synonym="iatA"
     CDS             17032..18426
                     /gene="ychO"
                     /locus_tag="ACHY5W_002080"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFS4446976.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAHSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSIEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18427..19077)
                     /gene="narL"
                     /locus_tag="ACHY5W_002081"
     CDS             complement(18427..19077)
                     /gene="narL"
                     /locus_tag="ACHY5W_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFS4446977.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19070..20866)
                     /gene="narX"
                     /locus_tag="ACHY5W_002082"
     CDS             complement(19070..20866)
                     /gene="narX"
                     /locus_tag="ACHY5W_002082"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFS4446978.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEEDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGILPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21205..22596
                     /gene="narK"
                     /locus_tag="ACHY5W_002083"
     CDS             21205..22596
                     /gene="narK"
                     /locus_tag="ACHY5W_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFS4446979.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5594 a         5653 c         5850 g         5606 t
ORIGIN      
        1 ttgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
       61 caaaaaacat cgatattcat ttttggtcaa tgggttttct ggcctatcgt tattatcgtg
      121 ctattggtta tcattttcat cttgccagga tcgcacgtaa tctcattatt cggggacttt
      181 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc
      241 aatattgatc agctgtgaag ggggtgatgt gtagtggaac aaaaacttac gttatatcac
      301 tactaatgag atatccgtca tctgaacact gatgtcttta attattctgg tggttcactg
      361 taggcaataa gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa
      421 aaatccacgc aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa
      481 cctcatgaga caacaaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt
      541 aacgatgtaa tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc
      601 tttgtaagtg atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa
      661 atcagtatgg attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc
      721 acgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc
      781 acggatcaca cggttcaggc ccggctcttc aagcccaagc tcctgcataa actcgtcacg
      841 ttcttcgtcg tccagttcgg caatgtctgc ttcaacagca gcacaaaccg gaaccacaac
      901 agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc
      961 gtcttcgttg acgttggcga tgtacattgt tggttttagc gtcaggaagc tcaggtaacg
     1021 aatagccgct ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag
     1081 ctggggcagg catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt
     1141 ggctttcttc tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag
     1201 ttcggtattg ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat
     1261 gttgtcattt tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt
     1321 caggaactgg ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc
     1381 gacaaattcc atggtcgtgg gaagcgtacg ctgcggtttt acgatttcag ccagttgatc
     1441 caggcgaggg tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa
     1501 gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt
     1561 gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata
     1621 atcaacctgt tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg
     1681 cactcggcaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc
     1741 gttgctttgg tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg
     1801 gcttcatcaa ttaacttctg ttcactaata ggcggtttgc ctaacacaaa accaacaact
     1861 ttatttttat cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta
     1921 cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta
     1981 aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga
     2041 ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta
     2101 gtcgggacta acaggcggac atcttcgcct ccaagggtga ctcgcgaagt ataaccaaag
     2161 aatttagcct cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag
     2221 gcaccagcat tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc
     2281 aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg
     2341 ttgcggcccc gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga
     2401 gtagactatt agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag
     2461 ggttgtaatt gtgatcacgc ccgcacataa cccacagggt gttgtctata ctttacacat
     2521 aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc
     2581 aaaaacgctt cgttactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtt
     2641 ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca
     2701 catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt
     2761 ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gccactatga caaacgttgc
     2821 cgccgtagcg ataatcgccg tcatagctaa caaataatgc agttttctga ccagtcagtt
     2881 tgcgctgact ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa
     2941 gaacgcgagg cgtcccggga tcggttgaat gcctgcgcga gccatagtgc gcagtggctg
     3001 gaattccacg ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg
     3061 gaacgcatca agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat
     3121 ccgtttgcct tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa
     3181 cagcgggcca acaacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc
     3241 caggcgagtc atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac
     3301 gctgatggca ataaccatat caaacagcac ggtcagcgac atgcacagca gcatgacaat
     3361 gatgtcatct ttcggtgcat gacgcagcaa gtcgaccact ttatgcgctt cactcatgtt
     3421 ccacgccacc atcaacagca gggctgccat agcggaaagc ggcagccagg agagcagcgg
     3481 tgccagtacc agcagggcaa gaataaccag aatagagtgg atcaccgccg agatagggga
     3541 agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagcggtag cggtaatacc
     3601 accaaagaac ggagcgatga tattccccag tccctgtcca accagttcgc tattcgcctt
     3661 gtgcttcgtc ccggtcatac catccagcac cacggcgcag agcagagatt cgattgcgcc
     3721 gagcattgcc attgagaatg ccgcaggcag cagtgtgcga atagaatccc aggttagcgt
     3781 gaattctgaa ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc
     3841 gttaccctga gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg
     3901 tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg
     3961 accgggtaaa cgaatgccca gacgcggcca gaaaacaaga atacctagcg tcacaatgcc
     4021 aatggcagca tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg
     4081 tagataatgt tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt
     4141 accgatggtg atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc
     4201 aatcaggcga ccaaagcgtg ccagacccat cagaatcaaa aagatccccg acagcaaggt
     4261 cgcaaccagc agtcctgcca gtccaaactg ttgcgacacg ggatagagaa ttaccacaaa
     4321 tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc
     4381 cgcaacagct gcggtatata aaccgtactg gggtgccaca ccactaccaa tagccaacgc
     4441 catcgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa
     4501 ccgtgcggca gtatattttt ctttccagca agcgtcgatc agagcgcgga aaggcatcac
     4561 atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac
     4621 taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg
     4681 ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt
     4741 cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt
     4801 tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa
     4861 ttacagagtt acgcaggtta ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg
     4921 caaatacacg tttagcacca cgttctttca gcgcttcagc agctttacac agcgtaccgc
     4981 cagtgtcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat
     5041 gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat
     5101 cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca
     5161 caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata
     5221 cgttatcaac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca
     5281 gcacacggtc aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta
     5341 cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg
     5401 tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt
     5461 tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt
     5521 cattaatttg tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg
     5581 aagtgtacag gcggttggca atacgttgtg ctagttccgg ggtggcgtta ccagcaaaaa
     5641 gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc
     5701 gaaaactgtg cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa
     5761 cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc
     5821 tgtgcaatgg ggaaagatta gcgcctttcg ccacaaagcc attgagccat tccggggctt
     5881 gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg
     5941 tcccagtcag gcgcgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc
     6001 gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt
     6061 ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg
     6121 gaatacttac accagggtgc gccaccagat accacttctc tggcggagcc accggcgtta
     6181 gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg
     6241 cgcccagcgt cagccccatt tccgccagct catccatgct tagtccgcat tgccagagat
     6301 gatttaatgc caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca
     6361 tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt
     6421 ctgccgcagt tttcatcaat aaccgcgctg cgcgaacgat caggttatct tcatgttcca
     6481 cgccttcaac gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg
     6541 tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct
     6601 gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta
     6661 tttcactatc cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat
     6721 attggctggc atcgcaggtt gcgttttggt gtcataacca ccgtaaacaa ctttccagtt
     6781 tttgccattc tggctgtagg taatttcgct caggcgatat tgatcgtcca gtttgtagtc
     6841 ggttgcatca cccggtaaac ctaaaatcca ctggcgcaaa ctgttgagcg gaattggcat
     6901 tccagtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt
     6961 gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg
     7021 gttagtgagc agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta
     7081 cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt
     7141 gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt
     7201 gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag
     7261 acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc
     7321 cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat
     7381 ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc
     7441 ctgtatgatg caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc
     7501 cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg
     7561 ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag
     7621 ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag
     7681 gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa
     7741 gatctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt
     7801 gttgccagcg gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa
     7861 aaagcgtttg ccgattcgca aaaaggccat atgaaggcca gcgaactgga acgcatgttc
     7921 cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagcgct
     7981 gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg
     8041 gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc
     8101 gaacataaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg
     8161 gcagatgaag tcggcgcgga agtgattgcc ctgagtgata tcgacgaacg tctgcgcgaa
     8221 gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg
     8281 gagcgcgcat taaaaagccg tcgcaaccaa ccaatgctgt tggtggatat tgccgttccg
     8341 cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag cgttgacgat
     8401 ctgcaaagca tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa
     8461 actattgtcg ctcaggaaac cagcgaattt atggcgtggc tgcgtgcaca aagcgccagc
     8521 gaaaccattc gcgagtatcg cagccaggca gagcaagttc gcgatgagtt aaccgccaaa
     8581 gcgttagcgg cccttgagca gggcggcgac gcacaagcca ttatgcagga tctggcatgg
     8641 aaactgacta accgcttgat ccatgcacca acgaaatcac ttcaacaggc cgcccgtgac
     8701 ggggataacg aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc
     8761 attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg
     8821 aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg
     8881 ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc
     8941 gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg
     9001 atgatcctga aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg
     9061 agcaactgga acagcaatta caggttctgt tactgccaaa agatcctgat gacgaacgta
     9121 acgccttcct cgaagtccga gccggaaccg gcggcgacga agcggcgctg ttcgcgggcg
     9181 atctgttccg tatgtacagc cgttatgccg aagcccgccg ctggcgggta gaaatcatga
     9241 gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg
     9301 gtgtgtatgg tcgtctgaaa tttgaatccg gcggtcatcg cgtgcagcgt gttcctgcta
     9361 cggaatcgca gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactgc
     9421 ctgacgcaga actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt
     9481 caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc
     9541 cgaccgggat tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagctaaag
     9601 cactttcagt gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg
     9661 aagcgtctac ccgtcgtaac ctgttgggga gtggcgatcg cagcgaccgt aaccgtactt
     9721 acaatttccc gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg
     9781 atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg
     9841 ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaatact ggttacgtga
     9901 agcaataaac caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga
     9961 gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgagacgc agctgactga
    10021 cgaacaatgt cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc
    10081 tcatttaacc ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt
    10141 aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca
    10201 accttgccgt attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag
    10261 cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc
    10321 acaacgtaat gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt
    10381 tagcgcgcta gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga
    10441 gcaggaccca catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc
    10501 ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt
    10561 atccggcggc tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaggc
    10621 atttatcctc gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg
    10681 tgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt
    10741 attagtatcg cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag
    10801 catcctcagg catttgcgcg ctggacgcgc attgtgccgc cggttgtcga tacggtgtta
    10861 ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca
    10921 cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca
    10981 ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg
    11041 gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc
    11101 atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg
    11161 gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt
    11221 ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa
    11281 aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt
    11341 ctttccgatg cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca
    11401 ttaggtgcgg ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt
    11461 ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat
    11521 ccttttaacg gtgaatcgtt aagcgaacat atgctggacg tatggttaaa gggaaatatc
    11581 agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc
    11641 cgcaaattac tggacacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg
    11701 ttacgcacca gcgaagcttt attacaattc aaccctgaag atccctatga aattcgcgat
    11761 cgcgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat
    11821 ttcgttgaac aatgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac
    11881 atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg
    11941 atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt
    12001 tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg
    12061 agcactacgt aaccgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg
    12121 acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga
    12181 aaatcttcca ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac
    12241 caagtcaggc acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg
    12301 ctcgccagac tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga
    12361 aaccacagtt tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg
    12421 gcaacgaaaa agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg
    12481 tcgatatgct gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg
    12541 acgtgaccca cgcattgcaa tgccgcgatc cttttggcgc agcttccggc ggtcgtcgtg
    12601 ctcaggtggc tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg
    12661 aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta
    12721 aactggaacc gttcctcaag cagatgaaag cgcttgatga tctggtgaaa ggtttcgaag
    12781 aactggatac cagcaagtaa tcttttttgc tggaaaaata aagtcttagc gttctgcgtt
    12841 aagacttttt tcatgggtgc cggatacaaa aaaggccgca ggctgttacc ctgcggccgg
    12901 tttcgggcgc atattgccat cacggcagcc tgacgcccgt tttcgcctta cttccggtta
    12961 cgccaccagc cgacaatcgc tgcggtaata attcccgcca ggatcggtgc tgccaggtcg
    13021 tgccagaaaa tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg taatgacaac
    13081 attttgcggc tattcttgaa ttgttgatgg ttcaagatta gcccccgttc tgttgtcagg
    13141 ttttacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
    13201 gcccccgcaa cattgcgcct caccggataa cgccggcttg gtgtggatac tacgactcaa
    13261 ttcatcttca cttcatccct gaaatgtttg caatgaagag tgcattccgg tttttcaaca
    13321 gctgttacag tcgtttcatg agtgctctgg atgatgcttc cagctcgggt tgccaatatt
    13381 tacttgtgga agtgataaag acaaaaatgg ccgcaggctg ttacccctgc ggccggtttc
    13441 gggcgcatat tgccatcacg gcagcctgac gcccgttttc accttacttc cggttacgcc
    13501 accagtcgac aatcgctgcg gtaataattc ccgccaggat cggtgccgct aggtcgtgcc
    13561 agaaagtcat ggcaaactgc gcgagcgtca tatagccgcc ttgttgtaat gacaacattt
    13621 tgcggctatt cttgaagtgt ctggtttcaa gattagcccc cgttttgttg tcaggtttta
    13681 cctctcaacg tgcgggggtt ttctctttcc agcaaccaat gccaccaggg ataaagcccc
    13741 cgcaacattg cgcctcaccg gataacgccg gcttggtgtg gatactacgt ctcaattcat
    13801 cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt caacagctgt
    13861 tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg acctgtccag
    13921 gtagtatgaa gaaatacaat aagcaggaat ttatattatc tcccccgatt tccgtcatcg
    13981 gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca gtgccagatg
    14041 cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc acagcactaa
    14101 agaggccacc atcaccacca tttctggcgc accaagtgca aactgcaatt cgttacccgt
    14161 cataaatgca attagcgtga cgacaggtac ggtaagcgaa atggttgcta acactgaacc
    14221 aaagaacaga ttcatcgcgc gctgaacctg gttgttcaac actgctttta atgcacctaa
    14281 accttccggc gacagaatca acagtgccac caggaagcca gtaaaggcga caggggcatt
    14341 catgctgtcg agcaaagtct ccagcgggct ggcgttcatt ttggtgactg caataacggc
    14401 aatcaggtgg ataatcaacc agatagcatg ccacaggctg ctatgggcag acggtttacc
    14461 gtgatgcggg tcgtcatcat cactgtcatc ttcgtgctcg tagacaaaca aactttgatg
    14521 cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta atgctaccag
    14581 taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata ccagtacgat
    14641 tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat tcatatattg
    14701 ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac cggtaacaat
    14761 cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc cggttgccat
    14821 taaagctgaa atcaaactaa cttcaagaat aaccactgaa aggctaagaa taagcgaacc
    14881 gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac taaaggcgct
    14941 acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta gtgtctggct
    15001 gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg aagtctcctt
    15061 gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt tgcagatgaa
    15121 tttgtcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg atatcggtaa
    15181 cattttacga aattttaccc cgctgtcgat tttttactca ttggggcata aaaataagta
    15241 atacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacac gaaaattctt
    15301 atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg taaaaggagt
    15361 caacgatgcc gtataaaacg aaaagcgacc tgccggaaag cgtaaagcac gttctaccgt
    15421 ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa tataaagata
    15481 aaaaagatcg gcgtgatgac gctagtcgcg aagaaaccgc gcataaagtg gcctgggctg
    15541 ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa tcgtaaaacc
    15601 ggtagcttag ttaaagctat tcgtgcgatg ttgccttgca agtggtccgc ggattgcata
    15661 ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac caaaaagttg
    15721 tcgcagggaa gcatgcagtg gcggaggtgt aaggtgataa cgcgtgattt cttgatgaat
    15781 gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc agcagaacaa
    15841 cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc agtgtggatc
    15901 ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc gtgcaccggt
    15961 acactggttg gatggcatcg tgcgttttgc ctgcgcctga ccgccgggcg cgggactgcg
    16021 caccagccgg gacggatgct tgcactgaaa gagggcggac gcaccacagg cgtcgcctat
    16081 cgactgcctg aagagacgct agagcaggaa ctaaccctgt tgtggaagcg agagatgatt
    16141 accggctgtt atctgccaac ctggtgccag cttgatcttg atgatggacg catagtaaac
    16201 gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac tcgcgctcag
    16261 gtcatagcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc acaatacctg
    16321 ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct gaatgatttg
    16381 ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt acggccggga
    16441 ttcgcctgag taaacttccc tcatagtggg gcgtcagacg ccccctcaaa cattaaaatg
    16501 tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta ccgatttcca
    16561 ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt ttgcacaatt
    16621 tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg tagccttccc
    16681 ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catcaggaac agacgcagat
    16741 ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta aacaaggatt
    16801 cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt tactcctgta
    16861 ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg accaatcggt
    16921 cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg ccagtaatcg
    16981 ttattcttta aggctatggt ttttcatttt ttaccggaag ttaccgacgt tttgagccgt
    17041 ttcgttcctc gcattattcc gttttattta ctcttgctgg cggcaggcgg tacagctaac
    17101 gcacactcta ccttcgagca aaaagcggca aatccctttg ataataacaa tgatggtctg
    17161 ccggatttag gcatggcacc tgaaaatcat gatggggaaa aacactttgc tgaaattgtg
    17221 aaagatttcg gcgaaaccag tatgaatgat aacgggctgg atactggcga gcaggcaaaa
    17281 gctttcgcat tgggaaaagt ccgcgacgcg cttagtcaac aggttaatca gcacgtagag
    17341 tcttggctat caccgtgggg aaatgccagt gttgacgtca aagtggataa cgaaggacat
    17401 tttaccggca gtcgtggaag ctggtttgtg ccgttacaag ataatgatcg ttatctcacc
    17461 tggagccagc ttggtcttac tcagcaggat gatgggttgg tgagcaatgt gggcgttggg
    17521 caacgctggg cgcgcggcaa ctggctggtg ggttataaca ctttttatga caacttgctg
    17581 gacgaaaatc ttcagcgagc gggctttggt gccgaagcgt ggggcgaata tttgcgacta
    17641 tcggcaaact tttatcagcc atttgctgca tggcatgaac agacagccac gcaggaacaa
    17701 cggatggcgc gcgggtacga cctgacagcc cggatgcgca tgccgttcta tcaacacctc
    17761 aataccagtg tcagcataga acagtatttt ggtgatcgtg ttgatttgtt taactctggt
    17821 acgggttatc acaatcccgt cgcgttgagt ctgggattaa attacacccc tgtgccatta
    17881 gtcactgtga cggcccagca taaacagggt gaaagtggcg agaatcaaaa taacctcgga
    17941 ctgaatctta attaccgctt tggtgtaccg ctcaaaaaac aactttctgc gggcgaggtt
    18001 gccgaaagtc agtcgttacg tggtagtcgc tatgataatc cgcagcgaaa taatctaccg
    18061 actcttgagt accgacagcg aaaaacgtta acggtgtttt tggcgacacc gccgtgggat
    18121 ctaaaacctg gcgaaacagt gccgctgaaa ttacaaatcc gcagtcgtta cggtattcgg
    18181 caactgattt ggcagggcga tacgcagata ttaagtttga cgccgggcgc acaagccaac
    18241 agcgcggagg gctggacgct gatcatgcct gactggcaga acggggaagg ggcgagcaat
    18301 cactggcgat tgtcggtggt ggtggaagat aaccaggggc agcgtgtctc ctccaatgag
    18361 atcacgctaa cgcttgtcga accgttcgac gcattgtcaa acgacgaact gcgctgggaa
    18421 ccgtaatcag aaaatgcgct cctgatgcac ccataccgct gcttccacgc gagacttgag
    18481 cttcattttc ttcagcatgt gcttgacgtg cacttttact gtgctttcgg tgatgtccag
    18541 gcggcgggca atcatcttgt tcggcaaacc ttgggcaatc agcttgagaa tatcgcgctc
    18601 gcgtggggtt aattggttaa catcgcgctc agtcgtggca cggttagcgc gcaagctggc
    18661 ggccagaaca ggcgttaatg cttcgcttaa taccatttcg ccagcagctg cctgatgcaa
    18721 tgctttcagc agatcttccg gttccatatc ttttaacaga tagccatccg cgccgcgttt
    18781 cagtgcggtg accacatctt cttcatggtt agagacgctg aataccacaa tgcgccctga
    18841 gagggacttt tcgcgcagtt tatccagcgt ttccagaccg ttcatgccgg gcatattgag
    18901 atctaacagg atcagatcgg gatcaagaga ttccgccagt tcaataccct gttcgccatt
    18961 actcgcttcg ccaaccacgg tgatatctgg tgccatactg ataagctgtt ttacgccagt
    19021 tcgcagcatc gggtgatcgt caatcagcag gatagtagcc ggttcctgat tactcatggg
    19081 tatctccttg gacgtctgtg aaagtttttt cgggaataaa ggtgaccacc acttcggtgc
    19141 cacctgattc acgacggcgg acgcggcaat cgcctcgtaa actttgcgca cggtcgcgca
    19201 ttattatcat gccgtagtga ttgctgcgga tggcattttc aggcacgccg cagccgttat
    19261 cctggacggt cagtttgacc tgattatcgt tttgcgccac cgtcaccacg acctcactcg
    19321 cttgcgaatg tttgagggcg ttacttaatg cctcacgggc aatttgcaac aagtggattg
    19381 cctgatgcga aggcaccaga cgaggcggca attgataatc cagcttcacc gggaagccaa
    19441 atttggcgct gtactcttcg caactcgcct ccagcgccgg acgtaatcca ggctcggtga
    19501 gctgcaagcg gaatgtggtg agcaattcac gcaactgcgc ccaggatgca ttcagttcgt
    19561 tacggatctg acttaacagt tcgcggctgc tttctggcag cgcatcgccc tgcatctgta
    19621 aacaactcac ctgcatcttc atgcaagaga gagattgggc aatagaatca tgcagttcgc
    19681 gcgcaatggt ggcacgctct tccatcacga tcaactgttg ctgacgttcc tgatgccgat
    19741 ccagcgccag cgtggcggtg agttgttcaa ccagggtatc caccagttgt tgttgatcat
    19801 ggctaagatg acgcccctgc ggcagggtcg ccagcaaaat accgtactgc gtatgagagt
    19861 cagccagccg ccacttcagg gtcgtgccgc gatcaccaac gggtaatatg ccgcgcgggc
    19921 agagctggca gcctttatca tcacaagtca tatctggctg gcaggtaaac tcctgatgat
    19981 tctcttcatc atcagtgtca tacacccgca gttcgatatc acgtagcagg gttaaattct
    20041 gtaagccgtt gagtacaggt gacaggcgtt cacacagcgg ggcgcgggaa tgcaaacggc
    20101 ggttagcctg ccataaaaaa gagaggatct gatttttatg ctccagcccg gcggttttct
    20161 cctgaacccg ctgctcaagt acggcataac tttcggccag ttctgcagac atattgttca
    20221 acgcagttcc aagcatcgcc atttcgttgc gcccgctgat gtttgcgcgt tgggtaaaat
    20281 cgcgatggct gacggcactc gccattgcca gcagttgccg ccacggttgt agcagtcgcg
    20341 cccgcaacca gataatagtg aacaccagta aaagtgccat aaataccgcc attacccgat
    20401 ggaccagtac cactgtctca atgcgcattt ccgtggtgcg gtcaaaacca gatactagct
    20461 gatcaagccc ggcaacaaac tggctgacat ccgctgacac cgtttctcgg ttttgtgcac
    20521 gcatcagcgc agggatcagt tcattacgcc agtaatcttg taaaccctgt aattgcgcca
    20581 gttgtccgtc tcgttctgct gctcgagtca actcggcgct aaatgccgtt tgttccatct
    20641 ctttaattaa gggcttgtct tcctcgctta atggaactgc cgccaacaga cggtaacttt
    20701 gcatgcgcag cgatcccgct ttgttgatcg catgggcgct gccctgaacg ccttgcacca
    20761 gccagccaga aaccgccatc cctgccagtc caatagcagt agaaagcaac acaataagcg
    20821 caacctgatt aaccagggtg agcggagaga gacaacgttt aagcatgtaa acctcttcct
    20881 tcaggcttta aatgagcaat aaccttaatg aatgtgacga tacattctgg aatggcagta
    20941 ttctcggcta ttggctgaag tatacccata cccggaaaga gttactcctt atttgccgtg
    21001 tggttagtcg ctttacatcg gtaagggtag ggattctaca gcaccgtgaa aaatctcata
    21061 atttttatga aatcactgta ctcactatgg gtaatgataa atatcaatga tagataaagt
    21121 tatcttatcg tttgatttac atcaaattgc ctttagctac agacactaag gtggcagaca
    21181 tcgaaacgag tatcagaggt gtctatgagt cactcatccg cccccgaaag ggctactgga
    21241 gctgtcatta cagattggcg accggaagat cctgcgttct ggcaacaacg cggtcaacgt
    21301 attgccagcc gcaacctgtg gatttccgtt ccctgtctgc tgctggcgtt ttgcgtatgg
    21361 atgttgttca gcgctgttgc ggtgaactta ccgaaagtcg gttttaattt tacgaccgat
    21421 cagctattta tgttgactgc gctgccttcg gtttctggcg cgttattacg tgttccatac
    21481 tcctttatgg ttcctatctt cggtggtcgt cgctggacgg cgttcagcac cggtattctg
    21541 attattcctt gtgtctggct gggttttgcc gtgcaggata cctccacgcc ttatagcgtc
    21601 ttcatcatca tctctctgct gtgcggcttt gctggcgcga acttcgcttc cagtatggca
    21661 aacatcagct tcttctttcc gaaacagaag cagggtggcg cgctgggtct gaatggtggt
    21721 ctgggcaaca tgggcgtcag cgtcatgcag ttggttgctc cgctggtggt atcactgtcg
    21781 attttcgcag tatttggtag ccagggcgtc aaacagccgg atgggactga gctgtatctg
    21841 gcgaatgcgt cctggatatg ggtgccgttc cttgccatct tcaccattgc ggcgtggttt
    21901 ggcatgaacg atcttgctac ctcgaaagcc tccatcaagg agcagttgcc ggtactcaaa
    21961 cggggtcatc tgtggattat gagcctgctg tatctggcaa ccttcggctc ctttatcggc
    22021 ttctccgcgg gctttgcgat gctgtcaaaa acgcagttcc cggatgttca gattctgcaa
    22081 tacgctttct tcgggccgtt tattggtgcg ctggcgcgtt ctgcaggtgg tgcattatct
    22141 gaccgtctgg gcggaactcg tgtcacgctg gtgaacttta ttctgatggc gattttcagc
    22201 ggcctgctgt tcctgacctt accgactgac gggcagggcg gaagcttcat ggcgttcttc
    22261 gcggtcttcc tggcgctgtt cctgacagct gggctgggta gtggttccac tttccagatg
    22321 atttcagtga tcttccgtaa actgacaatg gatcgcgtga aagcagaagg gggttctgac
    22381 gaacgtgcga tgcgtgaagc ggcaaccgac acggcggcgg cgctgggttt catctctgcg
    22441 attggcgcga ttggtggctt ctttatcccg aaagcgtttg gaagctcgct ggcattaacg
    22501 ggttcgccag tcggcgcaat gaaggtattt ttgattttct atatcgcctg cgtagtgatt
    22561 acctgggcgg tatatggtcg gcattctaaa aaataaatct taatatttga ttatcctttg
    22621 cgcggcatga tgtcgcgctt tttttatgcg tcatttagtt acaacatact aatgttatat
    22681 ggtttatttc gccggattta att
//
LOCUS       DAZUGK010000063        22616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZUGK010000063 DAZUGK010000000
VERSION     DAZUGK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.063"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..598
                     /gene="alaE"
                     /locus_tag="ACHY5W_002084"
     CDS             149..598
                     /gene="alaE"
                     /locus_tag="ACHY5W_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFS4446980.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(635..979)
                     /gene="ygaC"
                     /locus_tag="ACHY5W_002085"
     CDS             complement(635..979)
                     /gene="ygaC"
                     /locus_tag="ACHY5W_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFS4446981.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1131..1460
                     /gene="ygaM"
                     /locus_tag="ACHY5W_002086"
     CDS             1131..1460
                     /gene="ygaM"
                     /locus_tag="ACHY5W_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFS4446982.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1610..2944)
                     /locus_tag="ACHY5W_002087"
     CDS             complement(1610..2944)
                     /locus_tag="ACHY5W_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4446983.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWVRVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGLPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3032..3463
                     /locus_tag="ACHY5W_002088"
     CDS             3032..3463
                     /locus_tag="ACHY5W_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4446984.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3672..3917
                     /gene="nrdH"
                     /locus_tag="ACHY5W_002089"
     CDS             3672..3917
                     /gene="nrdH"
                     /locus_tag="ACHY5W_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4446985.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3914..4324
                     /gene="nrdI"
                     /locus_tag="ACHY5W_002090"
     CDS             3914..4324
                     /gene="nrdI"
                     /locus_tag="ACHY5W_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4446986.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNNEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4297..6441
                     /gene="nrdE"
                     /locus_tag="ACHY5W_002091"
     CDS             4297..6441
                     /gene="nrdE"
                     /locus_tag="ACHY5W_002091"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4446987.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYPRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWQALRT
                     SMLLARERGQTFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6451..7410
                     /gene="nrdF"
                     /locus_tag="ACHY5W_002092"
     CDS             6451..7410
                     /gene="nrdF"
                     /locus_tag="ACHY5W_002092"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4446988.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRSDEPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILTALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7766..8968
                     /gene="proV"
                     /locus_tag="ACHY5W_002093"
     CDS             7766..8968
                     /gene="proV"
                     /locus_tag="ACHY5W_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFS4446989.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKIPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8961..10025
                     /gene="proW"
                     /locus_tag="ACHY5W_002094"
     CDS             8961..10025
                     /gene="proW"
                     /locus_tag="ACHY5W_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFS4446990.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10082..11074
                     /gene="proX"
                     /locus_tag="ACHY5W_002095"
     CDS             10082..11074
                     /gene="proX"
                     /locus_tag="ACHY5W_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFS4446991.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11266..12450
                     /locus_tag="ACHY5W_002096"
     CDS             11266..12450
                     /locus_tag="ACHY5W_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4446992.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12574..13311
                     /gene="ygaZ"
                     /locus_tag="ACHY5W_002097"
     CDS             12574..13311
                     /gene="ygaZ"
                     /locus_tag="ACHY5W_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFS4446993.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13301..13636
                     /gene="ygaH"
                     /locus_tag="ACHY5W_002098"
     CDS             13301..13636
                     /gene="ygaH"
                     /locus_tag="ACHY5W_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFS4446994.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13727..14257
                     /gene="emrR"
                     /locus_tag="ACHY5W_002099"
     CDS             13727..14257
                     /gene="emrR"
                     /locus_tag="ACHY5W_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFS4446995.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14384..15556
                     /gene="emrA"
                     /locus_tag="ACHY5W_002100"
     CDS             14384..15556
                     /gene="emrA"
                     /locus_tag="ACHY5W_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFS4446996.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15573..17111
                     /gene="emrB"
                     /locus_tag="ACHY5W_002101"
     CDS             15573..17111
                     /gene="emrB"
                     /locus_tag="ACHY5W_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFS4446997.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGSGGGG
                     GGAH"
     gene            17369..18076
                     /locus_tag="ACHY5W_002102"
     CDS             17369..18076
                     /locus_tag="ACHY5W_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFS4446998.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAIAQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFAYGEQS"
     gene            18073..19185
                     /locus_tag="ACHY5W_002103"
                     /pseudo
     CDS             18073..19185
                     /locus_tag="ACHY5W_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(19234..19749)
                     /gene="luxS"
                     /locus_tag="ACHY5W_002104"
     CDS             complement(19234..19749)
                     /gene="luxS"
                     /locus_tag="ACHY5W_002104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4446999.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19899..21455)
                     /gene="gshA"
                     /locus_tag="ACHY5W_002105"
     CDS             complement(19899..21455)
                     /gene="gshA"
                     /locus_tag="ACHY5W_002105"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFS4447000.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLVTTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(21528..21956)
                     /gene="yqaA"
                     /locus_tag="ACHY5W_002106"
     CDS             complement(21528..21956)
                     /gene="yqaA"
                     /locus_tag="ACHY5W_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFS4447001.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVALLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(21953..22519)
                     /gene="yqaB"
                     /locus_tag="ACHY5W_002107"
     CDS             complement(21953..22519)
                     /gene="yqaB"
                     /locus_tag="ACHY5W_002107"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFS4447002.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5376 a         5933 c         5733 g         5574 t
ORIGIN      
        1 atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata tccagcgata
       61 tacgctatgc gaaaatgcag atgacaatga gatccactgt tttcatctcc attaacatcc
      121 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
      181 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
      241 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
      301 gaacatctta attgcatggc catacggtat gtaccgtgat ctgtttatgc gcgcggcacg
      361 caaagttagc ccgtcgggct ggataaaaaa tctggcggat atcctggctt atgtgacgtt
      421 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
      481 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
      541 cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggtaa aagcctgacc
      601 cttcttttcg cgactggcat cgccagtcgc gtctttaact ggcttcgcca aataaaccat
      661 tcaaataacg ttcaagcgca acacgcgaac taaagccatg cgggataccg taatgctcgt
      721 gtcgctcgcc tgctaaatag agcggaaatt gttgataatg atcgcaagtt ttaatatcgg
      781 aagcgggttc agccagtgtc gaactacgtt cttttaatgt tggatgaatt accagtgcgg
      841 tacgtcccat tcgcgcttcg cgattaacat agacataatt ttccccacgg cggtaaccat
      901 acgctttctg ggttaccaca tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct
      961 cgtctggtcg taaatacata tttgatcctc gttattatca accgcgggcc taccttacct
     1021 gattgcgcat tcacaacgct ttcagaaaag tccataaagc cgcgatgaac ctcagttaag
     1081 aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat atgtttaacc
     1141 gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat gtcaatcaat
     1201 tagctgacag cctcgaatct gtattgaagt cctggggcag cgacgccaaa ggggaagctg
     1261 aagccgcacg cagtaaagct caggcattgc tgaaagaaac cagggcgcga atgcatggtc
     1321 gtactcgcgt ccagcaagcc gcacgcgatg ccgttggctg cgcggattct tttgttcgtg
     1381 aaagaccctg gtgtagcgtg ggtacagcag ctgcggtagg tatttttatc ggtgcgttgt
     1441 tgagcatgcg taaatcgtag tgagacgatg acaagcagta gtctgacttc tgaaaagtac
     1501 tcagtgcgtc aacgccttgt ccgcctacag gcctgaaaaa catagcgcat caggggattt
     1561 tgccctagtc accagtctta taggaccccg aagcctggct tcggggttat taatttaaag
     1621 cactgcgcaa ttgctgtgct acctgtcgcg ccatactttc gctaacgata ttcgtaaacg
     1681 agagcaataa tcctccgtcc tcgcttgagc ggatacgcca gtcgctaagt gcctgtacgg
     1741 caagaccagc agcattggct ttatgcacga atgtcacgtc attacctgct agtttgatca
     1801 ccatctggat accgcctttt tgcggcacaa cctgaaatcc ctgctgcgta agtgcctgct
     1861 caatccactg ccgtcgctgg gcataatgct ggcgcatttt cttcagatgc cgccagaaat
     1921 gcccctcacg gatgaaatct gccagtgtgt tctgccacag tacgggtaca gcacatggtt
     1981 ccagtgacgc ctgctgccgg aattgcgaaa tctgctccac cggcaccacc agccacgcac
     2041 agcgcaatgc aggaaatagc gctttgctga atgttccggc ataaatcacc cgctgcggcg
     2101 cgtcgagact ttttagtgcc ggtaacggct taccgtgata gcgaaactca ctgtcgtaat
     2161 catcttcaat aatccatgcc tgactacgct ctgcccattc cagtatctga tacctacgcg
     2221 ctaaagagag tgccaccccc agcgggcttt ggtgtgccgg agtaatcagg gcaaaacgcg
     2281 catcaggata attttgtatt ccgcttgtga catccagtcc gttgtcatca accggcacag
     2341 gtaaaatttc cacaccgtgg cgagtgacaa tcgggcgaat cagcggtaag ccgggatctt
     2401 ccatccacat cccgtctccc ggttgcgcca gagcgtgcag aattaaagcc attgaggccg
     2461 cataaccgtg agtgataaag acctgctctg gctgacaatc aatcccacgt gaaacgcgta
     2521 aataatcaac aatagcctcg cgcaaggccg cctcgccgca gacatcgcct aatgccaaat
     2581 caaaacgcgt ctgggtacgc agacggcggc ccatcacccg cacccacaat tcacgcggaa
     2641 aaagatcaag tgcaggtagc cccatctgaa aaggcagcgg ctgctgactt tcaccagcaa
     2701 aagccacgac atccacagca gactgttgcg gatgtatctg ctcactgaca aaggtacccg
     2761 cctgtccccg cctgaccagc catccctgcg ccaccagctc agcataagca ttttcgacag
     2821 tggatcgaga gacacccagc tcctgcgacc aggtacggct cgagggtagc cttgcgccag
     2881 gtttcagctc tccttgctcg atagccgttt ttaactgacg ggcgatatcc tgataacgtg
     2941 gcatatggtc tgctttttta ggtaatttat ggctctcttt agcagtccat tatagtgcta
     3001 cattgcgggc gttaataaga ggagaaactt tatgactaca ttacgtcagc cttactacga
     3061 acttagcccg gcagtgtata acgcactggt gcaggccaaa acggcactgg aaaatagcac
     3121 gctggatacc acgttgatgg agttaattta tttgcgcgtc tcgcaaatca acggctgcgc
     3181 attttgtctg gagatgcaca gcaaagcatt gcgcaaatcc ggtgtgccac aacacaaact
     3241 ggacgccctg gcaggctggc gcgtaagcca tcattttgat gaacgcgagc gagcggcgct
     3301 ggcatgggcg gaatcagtga ccgatattgc cagaacccat gcggaagacg aggtttatca
     3361 gcctttgctt gagcatttca gcgcagcaga aatcagcgac ttaacgtttg ccatcgggct
     3421 gatgaattgt tttaaccgtc tggccgtttc catgcggatg taatttgctg ttgccgggta
     3481 tgcgcccggc aaaaaaacac aaaatcccct accccgttga gccactcacc ggtggcgtaa
     3541 tcaatctaaa attcccatat cttgtgtggt tgaatctttt ttcaactaca tctagtatct
     3601 ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg
     3661 aaatacgaat catgcgcatt actatttaca ctcgtaacga ttgcgttcag tgccacgcca
     3721 ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc
     3781 ctgaagcggc agaagcgttg cgtgctcagg gctttcgtca gttgccggta gtgattgctg
     3841 gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat ccagcgccac
     3901 acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa acacgcagcg
     3961 ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat
     4021 tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg
     4081 cgcggtgcca cgacaggtaa ttcgcttttt aaacaacgag cataaccggg cattgctgcg
     4141 cggcgttatt gcttcgggta atcgcaactt tggtgaggcg tatggccgcg ccggagatgt
     4201 gattgcccgg aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg gtacgcaaag
     4261 cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaacgc
     4321 ctgacgcagg aaacgatgga ttatcacgcg ctgaatgcga tgcttaacct ctacgatagc
     4381 gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat tgcgacgcat
     4441 gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg gctggtcaac
     4501 gaaggttact atgatgaaag cgttcttaat cgctaccctc gcgactttgt cattacgctg
     4561 tttgcccacg cacacaccag cggttttcgt ttccagacat tcctcggggc atggaagttt
     4621 tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga ttttgccgat
     4681 cgagtcacga tggtggcgct gacgctggca caaggcgatg aaacactggc gacgcagctc
     4741 actgatgaga tgctgtcagg acgctttcag ccagccacgc cgacattcct caactgcggt
     4801 aagcagcagc gcggcgaact ggtttcctgt tttctgctgc gtattgaaga caatatggag
     4861 tcgattggcc gggcggtaaa ttccgcacta caactgtcga aacgcggcgg cggcgtggca
     4921 tttttgctgt cgaatctgcg agaagcgggc gcgccaatta aacgtattga aaatcaatct
     4981 tctggcgtaa ttccggtgat gaaaatgctg gaagacgcat tttcctatgc caaccaactc
     5041 ggcgctcgtc agggggctgg tgcagtctat ttacatgctc atcatcccga tattctgcgt
     5101 tttctcgaca cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctg
     5161 ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg
     5221 ttttcgcctt atgacgtaga gcgagtttat ggcaagccgt ttgccgatgt ggccatcagc
     5281 caacactatg acgaactggt tgccgatgaa cgcattcgca aaaaatacct caacgcccgt
     5341 gatttcttcc agcgactggc agaaatccag tttgagtctg gctatccgta catcatgtat
     5401 gaagacacgg taaaccgtgc taaccctatc gccgggcgca taaatatgag taatctctgc
     5461 tcagaaattt tgcaggttaa cagcgcctca gagtataacg agaatctcga ctatgcccgc
     5521 acaggccatg atatctcctg caatttaggt tcgttgaata ttgcccacac catggattcc
     5581 cccgactttg cccgcacggt agagactgcc gtacgcggtt taacggcggt gtcagatatg
     5641 agtcatatcc gcagcgtgcc ctccatcgaa gccggaaatg ccgcctcgca cgccatcgga
     5701 ctggggcaga tgaatttaca tggttatctg gcgagggaag gcatcgctta tggttcgccg
     5761 gaagcactgg atttcaccaa tctctatttc tataccatca cctggcaagc actgcgtacc
     5821 tcgatgttgc tggcgcgcga acgcggtcaa accttcgccg ggttcaaaca gtcacgctat
     5881 gccagtggtg aatatttcag ccaatatctg caaggtaact ggcagccgaa aacggcgaaa
     5941 gttggcgaac tgtttgcccg cagcggtatt acgttaccta cccgtgagat gtgggcgcaa
     6001 ctgcgcgatg acgtgatgcg atacggcatt tacaaccaga atctccaggc ggtgccgcca
     6061 accggttcta tctcttatat caaccatgct acgtcgagta ttcatccgat tgtggcgaaa
     6121 gtagagatac gcaaagaggg caaaacagga cgcgtttact accctgcccc atttatgact
     6181 aacgaaaatc tggcgctgta tcaggacgct tacgaaatcg gcgcagaaaa gatcatcgac
     6241 acctacgcgg aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc
     6301 gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caaaggtatc
     6361 aaaacgctct attacatccg cctgcgtcag atggcgctgg aaggcactga aattgaaggc
     6421 tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac
     6481 tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc
     6541 tggctaccag aaaaagtgcc actatcgaac gatattcctg catggcagac actaagtgtc
     6601 gtagaacaac aactgacgat gcgcgttttt actggcctga cgctgctcga cacgctgcaa
     6661 aatgttatcg gcgcgccttc gctgatgccc gatgcgctca cgccccatga agaggcggta
     6721 ttatcgaata tcagctttat ggaagcggtt catgcccgtt cttacagttc gattttctcg
     6781 acgttgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcaccg
     6841 ttgcagcgaa aagctcagat tattcagcaa cattatcgca gtgatgaacc gctgaaaaag
     6901 aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg
     6961 tatttttcca gccgcggaaa gctaaccaat accgcagacc tgatccgtct gattatccgg
     7021 gatgaagcag tccacggtta ctacatcggc tataaatatc agaaaaacct ggaaaagatc
     7081 tcccaggcac gacgtgaaga attgaagagt ttcgccttcg atttgttgct ggaactctat
     7141 gacaacgagt tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg
     7201 aaagcgtttc tctgttacaa cgccaataag gcgttgatga atctgggcta cgaaccgcta
     7261 tttcccgcag aaatggcgga agtgaatccg gcaatcctca ccgcgctttc gccgaatgcc
     7321 gatgaaaatc acgatttctt ttccggttca ggctcttcat atgtgatggg aaaagcggtc
     7381 gaaacagaag atgaagactg gaatttctga gggggttatt ttcaaaaata tcactacctg
     7441 ctgcagggaa ataattcccg ccaaatagct ttatatcacg taaataattt gtggtgatct
     7501 acactgatac tctgttgcat tattcgcctg aaaccacaat attcaggcgt ttttttgcta
     7561 tctttgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat catgggaaat
     7621 tccggcgatt tgctcacatc aatattcatg ccacatttgc cctcaggggt tgcctcagat
     7681 tctcagtatg ttagggtaga aaaaagtgac tatttccatt gggtaatgta tcgacataaa
     7741 caaataacag gaatctttct attgcatggc aattaaatta gaaattaaaa atctttataa
     7801 aatatttggt gagcatccac agcgagcttt caaatatatc gaacaaggac tttcaaaaga
     7861 acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc tggccattga
     7921 agaaggcgag atatttgtca tcatgggatt atccggttcg ggtaaatcca caatggtacg
     7981 ccttctcaat cgcctgattg aacccacccg cgggcaagtg ctgattgatg gtgtggatat
     8041 tgccaaaata tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt
     8101 ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt tcggtatgga
     8161 actggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac tgcgtcaggt
     8221 cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg
     8281 tgtgggatta gcccgcgcgt tagcaattaa tccggatata ttattaatgg acgaagcctt
     8341 ctcggcactc gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc
     8401 gaaacatcag cgcaccattg tctttatttc ccacgatctc gatgaagcca tgcgtattgg
     8461 cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat
     8521 tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggtgttg atattagtca
     8581 ggtattcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc gtaaaatccc
     8641 tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgcg aatatggcta
     8701 cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc ttaaaaccgc
     8761 gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag cagtcgatgc
     8821 acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg cggtgcccgt
     8881 ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcgcgcttt
     8941 agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc acgccagcgg
     9001 cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc gacgactgca ccgactgacg
     9061 gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc
     9121 tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact
     9181 gggtcgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc
     9241 tcaacggttt ccagcaattg ctgctgggta tgccagcgcc agtggcgatt atcgttttcg
     9301 ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg gtttcgctga
     9361 ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt gactctggcg ctggtgttaa
     9421 ccgccctgct gttctgtatc gtcatcggtt tgccgttggg gatctggctg gcgagaagtc
     9481 cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg
     9541 tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc gtggtggtga
     9601 cgatcatctt tgctctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc
     9661 cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca
     9721 aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc
     9781 tggccctttc tatggtggtc attgcctcga tgattgccgt cggcgggctg ggtcagatgg
     9841 tacttcgcgg tatcggtcgt ctggatatgg ggcttgccac cgttggaggc gtcgggattg
     9901 tgatcctcgc cattatcctc gatcgcctga cgcaggccgt tgggcgcgac tcacgcagtc
     9961 gcggcaaccg tcgctggtac accactggcc ctgtcggtct gctgacccgc ccattcatta
    10021 agtaactctg cacttgcccg gtgacgccgg gcatatcacc ctgccaaaaa aaggaataac
    10081 aatgcgacat agcgtacttt ttgcgacagc gtttgccacg cttatctcta cacaaacttt
    10141 tgctgccgat ctgccgggca aaggcattac tgttaatcca gttcaaagca ccatcaccga
    10201 agaaaccttc cagacactgc tggtcagtcg tgcgctggag aaattaggtt ataccgtcaa
    10261 taaacccagc gaagtggatt acaacgttgg ctacacctcg cttgcttccg gcgatgcaac
    10321 cttcaccgcc gtgaactgga cgccactgca tgacaacatg tacgaagctg ccggtggcga
    10381 taagaaattt tatcgtgaag gggtatttgt taacggcgcg gcacagggtt acctgatcga
    10441 taagaaaacc gccgaccagt acaaaatcac caacatcgca caactgaaag atccgaagat
    10501 cgccaaactg ttcgatacca acggcgacgg taaagcggat ttaaccggtt gtaacccagg
    10561 ctggggctgc gaaggtgcga tcaaccacca gcttgccgcg tatgaactga ccaacaccgt
    10621 gacgcataat caggggaact acgcagcgat gatggccgac accatcagtc gctacaaaga
    10681 gggcaaaccg gtgttttact acacctggac gccgtactgg gtgagtaatg agctgaagcc
    10741 agggaaagat gtggtctggt tgcaggtgcc gttctctgca ctgccgggcg ataaaaatgc
    10801 cgataccaaa ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca tgcatatcgt
    10861 tgccaataaa gcctgggccg agaaaaaccc ggcagcagcg aaactgtttg ccattatgca
    10921 gttgccagtg gcagatatta acgcccagaa cgccattatg catgacggca aagcctcaga
    10981 aggcgatatt cagggccacg ttgatggctg gatcaaagcc caccagcaac agttcgatgg
    11041 ctgggtgaat gaggcgttgg cagcgcagaa gtaattttta ttcgggcgga taaggcgttt
    11101 tcgccgcatc cgccgttctg tacacaatgc ctgatgcgac gctggcgcgt cttatcatgc
    11161 ctacaaacca caccgcacat ttcaacaatc tatctttcat cccatattca tcaacatccg
    11221 ctattattga tttccagctt aatcatcacc tgatgaacaa aaataatgac taaacctaat
    11281 catgagctta gcccggcgct gatcgtgctg atgtctatcg ccaccggtct ggcggtcgcc
    11341 agcaactatt acgcccagcc attgctcgac accatcgcgc gtaacttttc cctttccgcc
    11401 agttcggcag gctttattgt taccgccgcg cagttgggct atgccgccgg tctgctgttt
    11461 cttgttcccc tcggtgatat gtttgaacgc cgccgcctga ttgtctcgat gaccttactg
    11521 gcagcgggcg gtatgttaat cactgccagc agtcagtcac tggcgatgat gatcctcggt
    11581 acggcattaa ccggtttatt ctcagtcgtg gcacaaattc tggttccact ggcagcgacg
    11641 ctggcttcac cggacaaacg cggcaaagtg gttggcacca ttatgagcgg tctgctgttg
    11701 gggatcttgc tggcacgaac cgttgccggg ttactggcaa acctcggcgg ctggcgcacc
    11761 gtcttttggg ttgcctcggt gttaatggca ctgatggcgc tggcattatg gcgtggtctg
    11821 ccacaaatga aatcagaaac ccacctcaat tacccacagt tgttgggttc cgtgttcagt
    11881 atgtttatca gcaataagat cctgcgcacc cgcgcgttgc tgggctgcct gacctttgcc
    11941 aacttcagca ttctctggac ctcaatggcc tttttgcttg ccgctccacc ttttaactac
    12001 agcgatggtg taattggtct gtttgggctt gcgggagctg ccggagcgtt gggcgctcgt
    12061 ccggcgggcg gttttgccga caagggcaaa tcgcaccaca ccacaacttt cggtctgctg
    12121 ctgctgttac tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt
    12181 atcggcattc tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta
    12241 atttatcgaa tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac
    12301 tttattggcg gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg
    12361 gctggcgttt gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga
    12421 ggttttcatc gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca
    12481 cttataaggg tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat
    12541 ttataatatt aacaatcgtt aagcgtacac tctatggaaa gccctactac acagcctgct
    12601 cctggttcgg cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat
    12661 attccggtgg cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa
    12721 agcgtttttt tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg
    12781 ctggcagcag ggagtagttt gtgggttgct gcactgaccg tcatggcaat ggatgttcgc
    12841 catgttttgt atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa
    12901 accgccctat gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg
    12961 gtacgcaata atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg
    13021 tcatcgtggg tctttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt
    13081 tatcccgccg ttgaagcagc attaggtttt atgcttccgg cactctttat gagtttcctg
    13141 ctcgcctctt tccagcgcaa acaatctctt tgcgttaccg cagcgttagt tggtgccctt
    13201 gcaggcgtaa cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc
    13261 ctcactgcgt taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct
    13321 gcttgggtta ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg
    13381 tgtgggtaat gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg
    13441 catcgcctcg atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac
    13501 acgccgtttc gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac
    13561 acgcagcatt atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt
    13621 gatggcgatt atataaccca caagaatcat ttttctaaaa caatacattt actttatttg
    13681 tcactgtcgt tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt
    13741 tacgcccatt gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca
    13801 ggagatcctt ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa
    13861 taaaatgctg aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga
    13921 gtctcaggaa aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg
    13981 taccaacgcg acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtga
    14041 aagcgataac gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt
    14101 gcgcgaggtt ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac
    14161 aacagaaaaa gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat
    14221 ggaacaagac ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc
    14281 agatttataa aagaaaaaat gactggccag catcgcaaca tgctggcctt tttggcaagc
    14341 aggtcggctc agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga
    14401 gactcaaacc ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct
    14461 tctcaccttg ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact
    14521 gcgtcacttc gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc
    14581 tcaggtgtct ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg
    14641 cgatgttctg gtcactctcg acccgacaga tgctcgccag acgtttgaaa aagccaaaac
    14701 tgcactggct tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca
    14761 ggcgaatatt gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg
    14821 tgtgccgctg ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc
    14881 cgttaccagt gcccaggcgc aactggacgt cgcgattcaa caatacaatg ccaatcaggc
    14941 gatgattctg gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt
    15001 acgtaacgcc tggctggcgc tggagcgtac tcgtattgtc agtccgatga ccggttatgt
    15061 ctcccgccgc gcggtacagc ctggggcgca aattagccca acgacgccgc tgatggcggt
    15121 cgttccagcc accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat
    15181 gcgtatcggt cagccggtca ctatcaccac cgatatttac ggcgatgatg tgaaatacac
    15241 cggtaaagtg gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca
    15301 aaatgcgacc ggtaactgga tcaaagtcgt tcagcgtctg cctgtgcgta tcgaactgga
    15361 ccagaaacag ctggagcaat acccgctgcg tatcggttta tccacgctgg tgagcgtcaa
    15421 taccactaac cgtgacggtc aggtactggc aaataaagta cgttccactc cggtagcggt
    15481 aagcaccgcg cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa
    15541 agctaacgct ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg
    15601 cgcaactggt cattatgacg attgcgctgt cactagcgac attcatgcag gtgctggact
    15661 ccaccattgc taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc
    15721 agggaacgtg ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg
    15781 gctggctggc aaagcgcgtc ggggaagtga aactgttcct ctggtcaacc atcgcctttg
    15841 ctattgcgtc gtgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg
    15901 tgattcaggg gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata
    15961 attacccgcc agccaaacgc tcaatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg
    16021 cgccaatttg cggcccaatc ctcggcggtt atatcagcga taattaccac tggggctgga
    16081 tattcttcat caacgtgcca attggcgtgg cggtggtgtt gatgacactg caaaccctgc
    16141 gcggacgcga aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg
    16201 ttattggtat aggcagcctg cagattatgc tcgaccgcgg taaagagctg gactggtttt
    16261 catcacagga aattatcatc cttaccgtgg tggcggtggt ggctatctgc ttcctgattg
    16321 tctgggagct gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact
    16381 tcaccatcgg ctgtttgtgt atcagcctcg cgtatatgct ctacttcggc gcgattgttc
    16441 tgctgccgca gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct
    16501 ctgcgccggt agggattatt ccagtgatcc tgtcgccgat tatcggccgc ttcgcgcata
    16561 aactggatat gcgccgactg gtaaccttca gctttattat gtatgccgtc tgcttctact
    16621 ggcgtgccta tacctttgaa ccaggtatgg attttggcgc gtcggcctgg ccgcagttta
    16681 tccaggggtt tgcggtggcc tgcttcttta tgccgctgac caccattacg ctgtctggtt
    16741 tgccaccgga acgactggcg gcggcatcga gcctctccaa ctttacgcgt acgctggcgg
    16801 ggtctatcgg cacgtcgata accacaacca tgtggaccaa ccgcgagtcg atgcaccatg
    16861 cgcagttgac tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac
    16921 tggaagggct tgggatgaca caacagcagg catcaggctg gattgcccag cagatcacca
    16981 atcaggggct gattatttcc gccaatgaga tcttctggat gtcggccggg atattcctcg
    17041 tcttgctggg gctggtgtgg tttgctaaac cgccctttgg ctcaggtggc ggcggaggcg
    17101 gtgcgcacta aggttgtcaa agggagctgc ggctcccttt ttatttgctc attctgtaaa
    17161 cggttgtttt atccactcat tcggtgtatc ttttcgccgc cccaaactct tccattcacc
    17221 attaaccggt tctgagcatt cgctcaggtt gttaattctc tttttccttc gccctcgctt
    17281 tcgcgggtga tactcacatt tttcttttaa ccccataact cgcaccctcg cgggcgtaca
    17341 tttattacac acaaaataat ggattattat gaatacccaa cggaaatacg ggagaacctg
    17401 gcattatcct ttctcccctg gcaccaccag cgatgaccgc attaacgccg attactggca
    17461 ggatttacag gccattgcgc aactggtgca cacagaaaaa ctcgacggcg aaaataactg
    17521 ccttaaccgc tacggcgtgt ttgcccgttc ccatgctgca cctacgcagt cggcatggac
    17581 ttataaaatt cgccagcgtt ggcaactgct gaaaaacgat ctcggcgatc tggaactgtt
    17641 tggtgaaaat ctctacgccg ttcattccat tgaataccgc gcgctggaac aggactttta
    17701 cctgtttgcc gttcgctgcc aggatatgtg gctgagctgg gaagaggtgc agttttacgc
    17761 cgctctgttt gatttcccct gcgttcccga aatctccggt ccgcagccgg ggaatgatga
    17821 aaaaagctgg caacgtgatt ttctggcact gaccaatgcg cgcggcgcgt tcgatccctg
    17881 ggatacacaa accgggcaac cctgtacgct ggaaggcatt gtcagccgga atcgtgatgc
    17941 gttttccgtc gctgactttt cgcataacgt cttcaagtat gtgcgcaaaa atcatgtcaa
    18001 aaccaccgtg cactggaaac gacactggca acgggcgcgg atggcccatg agttcgctta
    18061 tggagaacag tcatgatttg gcaacttacc gacgataaac gctggtcggc gctacgtcag
    18121 cgttttagct gggttgagga gatgcatcac acgccacaag acccggagca tcatggtgaa
    18181 ggcgacgtcg gcgtgcatac cgaaatggtg cttaacgccc tcgttactct gccagaattc
    18241 cagcaactcc ccgctcaaca gcaggaagtt ttatgggcgg cggcattatt gcatgatgtt
    18301 gaaaagcgca gcactaccgt gcaggaaaac ggacgtattc agtcgccggg ccatgctcgt
    18361 cggggcgaac tgacggcccg gctgattttg tggcgcgata tcccaacgcc gttcgtgctg
    18421 cgcgagcaaa tcgttgcgct ggtgcgtctg catgggttac cgctatggtt actggaacgt
    18481 ccggaaccgg agcgtttgct gctcacggct gcaatgcgca tcgacacgcg tctgctcgct
    18541 ttgcttgctc gtgccgatct gcttggtcgc caaagcccgg atcggcaatc aatgctggaa
    18601 cgcatcgatc tgttcgagct gttttgccag gagcaacagt gttggggaaa agtgcgctcg
    18661 ttcgtgtctg attccgcacg ctggcattac ctgacccatg agcaaagttc tcctgatttt
    18721 gttccctgag aggctgaacc ttttgaggtc attttgctgt gtggcttacc gggtatgggc
    18781 aaagatcgct atatcagtaa acagtgccag ggaattgacg ttatcagcct tgatgatatg
    18841 cggcggcgaa tcaatgccag cccggatgac aaaaccgcta ccgggcgtat tgtgcaacag
    18901 gcaaaagaag aggcgcgcgt ttttttacgc cagaaaaagc cgttcatctg gaatgcaacg
    18961 aacattaccc gccagctgcg tagccagctc atcagcctgt ttacggctta cggcgcacga
    19021 gtaaaaatcg tttatcttga agtgccatgg gcgcaatgga agcagcaaaa tgccagacgc
    19081 gagtatgcag ttcctgacgc ggtcgtgatg cggatggcct caaggctgga agttcctcaa
    19141 cctgatgaag cacatagcgt ggagtatcgg gtgattgaaa ggtaaaaaaa ccgggcggtg
    19201 gtgtcacctg cccggcttct tttgttccac tgactaaatg tgcagttcct gcaacttctc
    19261 tttcggcagc gccagttctt cgttgctgtt gatgcgcacg tcacgttcca gaatgctacg
    19321 cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt
    19381 cagctccgga atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca
    19441 ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg
    19501 gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat
    19561 aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca cttctttgtt
    19621 cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt
    19681 tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa
    19741 caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca
    19801 ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg
    19861 aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac
    19921 accgcaaacg gttcggtatc tgcggcttcc atttcctgct gacggcgttc agacgcctcg
    19981 cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg cagcagatta
    20041 cggtaggctt ctgcaaatgc tttgcctgtt ccgccaatac cagtatcaat catagacctt
    20101 aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag ttcatcacac
    20161 actttctgat acgcttcgcc gccgttaatg ctatccagcg tttgcgcgac gcgtttcaga
    20221 tcgcggaaca gatctttacc cacctgcggt aacgggaact gtgcggtttc gcagccgata
    20281 cccagcgtca gacccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta
    20341 caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac
    20401 aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaatgg gttgatgtcc
    20461 agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggcgactcg
    20521 ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca gttcgttttc aatctgcagc
    20581 acgttgctgt tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct
    20641 tccgatggcg ttttgattgc ttgtttaagg cccgctacgt actcgtaaag atcgttgaag
    20701 gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag
    20761 gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt
    20821 tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc
    20881 caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg
    20941 gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa
    21001 ttgtaatgca cgccggaaat ggtttgcatc agcgcgccat agcgattttt cagcccttca
    21061 cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc
    21121 tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg
    21181 cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat atcaccatcc
    21241 actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttatgcgtc
    21301 agtgcggaac ctaatgcttc aggatgacct gttgttacca gtgtgccatc agcattaaca
    21361 cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc
    21421 agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg
    21481 ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac
    21541 atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt accaaggcac
    21601 aaaaaaaaga ttactggccc ccaggagatg cgcatccatc ccgctaagag acacagtaaa
    21661 tcgccaacca cgggcatcca gcttaataat agcgcgactg caccatagcg tttcagccag
    21721 ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata
    21781 acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga
    21841 tgactgatcc cagaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt
    21901 gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca
    21961 agcgaacatc cacagcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat
    22021 cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg
    22081 tgtctggcgc gggtttatgg tgtttgacgt gatcggcagc gacgacggcg tcaaaataac
    22141 ggcgtaatcc caggtgagcc agcaatgcct cagcgatggc gctttcactc cccgttccta
    22201 cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga
    22261 cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggt
    22321 cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat
    22381 taagcgcaat catcgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc
    22441 acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac
    22501 ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt
    22561 aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataa
//
LOCUS       DAZUGK010000064        21924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZUGK010000064 DAZUGK010000000
VERSION     DAZUGK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.064"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..687
                     /gene="yehC"
                     /locus_tag="ACHY5W_002108"
     CDS             13..687
                     /gene="yehC"
                     /locus_tag="ACHY5W_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4447003.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            703..3183
                     /gene="yehB"
                     /locus_tag="ACHY5W_002109"
     CDS             703..3183
                     /gene="yehB"
                     /locus_tag="ACHY5W_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS4447004.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSEFVQVGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3199..4233
                     /gene="yehA"
                     /locus_tag="ACHY5W_002110"
     CDS             3199..4233
                     /gene="yehA"
                     /locus_tag="ACHY5W_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFS4447005.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLEGIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4315..4653)
                     /gene="rcnB"
                     /locus_tag="ACHY5W_002111"
     CDS             complement(4315..4653)
                     /gene="rcnB"
                     /locus_tag="ACHY5W_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFS4447006.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(4872..5696)
                     /gene="rcnA"
                     /locus_tag="ACHY5W_002112"
     CDS             complement(4872..5696)
                     /gene="rcnA"
                     /locus_tag="ACHY5W_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFS4447007.1"
                     /translation="MTECTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            5817..6089
                     /gene="rcnR"
                     /locus_tag="ACHY5W_002113"
     CDS             5817..6089
                     /gene="rcnR"
                     /locus_tag="ACHY5W_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFS4447008.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6181..6277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6312..7100
                     /gene="thiM"
                     /locus_tag="ACHY5W_002114"
     CDS             6312..7100
                     /gene="thiM"
                     /locus_tag="ACHY5W_002114"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFS4447009.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLQAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSAQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7097..7897
                     /gene="thiD"
                     /locus_tag="ACHY5W_002115"
     CDS             7097..7897
                     /gene="thiD"
                     /locus_tag="ACHY5W_002115"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4447010.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            7962..8780
                     /gene="yegX"
                     /locus_tag="ACHY5W_002116"
     CDS             7962..8780
                     /gene="yegX"
                     /locus_tag="ACHY5W_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFS4447011.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            8832..9578
                     /gene="yegW"
                     /locus_tag="ACHY5W_002117"
     CDS             8832..9578
                     /gene="yegW"
                     /locus_tag="ACHY5W_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4447012.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9552..10517)
                     /gene="yegV"
                     /locus_tag="ACHY5W_002118"
     CDS             complement(9552..10517)
                     /gene="yegV"
                     /locus_tag="ACHY5W_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS4447013.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQCLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10514..11518)
                     /gene="yegU"
                     /locus_tag="ACHY5W_002119"
     CDS             complement(10514..11518)
                     /gene="yegU"
                     /locus_tag="ACHY5W_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS4447014.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAIATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(11515..12792)
                     /gene="yegT"
                     /locus_tag="ACHY5W_002120"
     CDS             complement(11515..12792)
                     /gene="yegT"
                     /locus_tag="ACHY5W_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFS4447015.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNVTGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13049..14101
                     /gene="fbaB"
                     /locus_tag="ACHY5W_002121"
     CDS             13049..14101
                     /gene="fbaB"
                     /locus_tag="ACHY5W_002121"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFS4447016.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            14408..15262
                     /gene="gatY"
                     /locus_tag="ACHY5W_002122"
     CDS             14408..15262
                     /gene="gatY"
                     /locus_tag="ACHY5W_002122"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFS4447017.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15291..16553
                     /gene="gatZ"
                     /locus_tag="ACHY5W_002123"
     CDS             15291..16553
                     /gene="gatZ"
                     /locus_tag="ACHY5W_002123"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFS4447018.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVEMVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            16563..17015
                     /gene="gatA"
                     /locus_tag="ACHY5W_002124"
     CDS             16563..17015
                     /gene="gatA"
                     /locus_tag="ACHY5W_002124"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFS4447019.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17046..17330
                     /gene="gatB"
                     /locus_tag="ACHY5W_002125"
     CDS             17046..17330
                     /gene="gatB"
                     /locus_tag="ACHY5W_002125"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFS4447020.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKEFCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG"
     gene            17334..18689
                     /locus_tag="ACHY5W_002126"
     CDS             17334..18689
                     /locus_tag="ACHY5W_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS4447021.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            18737..19777
                     /gene="gatD"
                     /locus_tag="ACHY5W_002127"
                     /pseudo
     CDS             18737..19777
                     /gene="gatD"
                     /locus_tag="ACHY5W_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            19883..20656
                     /locus_tag="ACHY5W_002128"
     CDS             19883..20656
                     /locus_tag="ACHY5W_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4447022.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(20738..21637)
                     /gene="yegS"
                     /locus_tag="ACHY5W_002129"
     CDS             complement(20738..21637)
                     /gene="yegS"
                     /locus_tag="ACHY5W_002129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFS4447023.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     SVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         5754 a         5082 c         5584 g         5504 t
ORIGIN      
        1 agaggcatta aaatgaaagg attattatct ttactcattt tttctatggt ccttcctgca
       61 catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa taaagaagtg
      121 atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg gattgatgat
      181 ggcgatacgt cattaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg
      241 gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg
      301 cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc aaatagtcca
      361 gagcaagaag gtaagaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac
      421 cggccagcgg gtattgctcc ggtaaataaa gcgacattta aaaaattgct ggtaaatcgc
      481 agtggcaatg gtttggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc
      541 aaagctaata atgtcaaagt caattatgaa actattatga ttgccccctt agaaagtcag
      601 agtgttaatg tcaaaagtaa taatgcaaat aactggcatc tgaccattat cgatgaccat
      661 ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca
      721 cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc
      781 tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt
      841 gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa taagcaatgg
      901 cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt atcaagagaa
      961 gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca
     1021 tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt tcgtctcgat
     1081 ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaagtg gctatgttcc accggaaaac
     1141 tgggagcggg gtattaatgc gttttatacc tcttattatc tgagtcagta ttacagcgac
     1201 tataaagcgt cgggtaataa caagagtaca tatgtacgtt ttaacagcgg gttaaattta
     1261 ctggggtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa tccaggggtg
     1321 tggaaaagca ataccctgta tctggaacgt ggatttgccc aacttctcgg cacgcttcgc
     1381 gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag aggtgtgcgg
     1441 ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc acgggtgcag
     1501 gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt cgtttatcag
     1561 aaagaggttc ctcctggccc gttcgcgatt acagatttgc agttggccgg tggtggagca
     1621 gatcttgatg tcagcgtgaa agaggcggac ggttcggtaa ccacctatct ggtgccttat
     1681 gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc ggcgggtcgt
     1741 agccatattg aaggggcgag caaacaaagt gaatttgtcc aggtgggtta tcagtatggt
     1801 tttaataatt tattgacgct gtatggcggc tcgatggtcg cgaataatta ttacgcgttt
     1861 actttgggga ctggctggaa tacacgcatt ggtgccattt ccgtcgatgc cactaagtcg
     1921 catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac
     1981 aaatttgtga gccaaacgtc gacgcgtttt ggtcttgcgg cctggcgtta ttcatcgcgt
     2041 gattaccgga catttaatga tcacgtttgg gcaaacaata aagataatta tcgccgtgat
     2101 gaaaacgatg tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc
     2161 ttttctgcca atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg
     2221 ttatggcgag attactgggg acgtagcggc agcagtaagg attatcagtt gagttattcc
     2281 aataactggc ggcggataag ctataccctc gcggcaagcc aggcttatga cgagaatcat
     2341 catgaagaga aacgatttaa tatttttata tcgattccct ttgattgggg tgatgacgtt
     2401 acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga tcaggggttt
     2461 gcctcaaata atacgggatt atcaggaacc gttggaagcc gggatcagtt taactatggg
     2521 gtcaacctga gttatcagca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg
     2581 aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacaggct
     2641 ggagccagtg tttctggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt
     2701 ctttccgaaa cgtttgctgt gatgaatgcc ccaggaatta aagatgctta tgtcaatggg
     2761 caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat
     2821 cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc
     2881 aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat
     2941 cagcgcaagc cctggtttat aaaagcgtta agagcggatg ggcaaccatt aatgtttggt
     3001 tatgaagtca atgatatcca tggtcataat attggcgttg tcggtcaggg aagccagtta
     3061 tttattcgca ccaatgaagt accgccatcg gttaatgtag caattgataa gcaacaagga
     3121 ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag
     3181 taatagtcag gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt
     3241 atgcctgtga gctatgcggc atgttatagt gagttatctg ttcagcacaa cttggttgtt
     3301 cagggggatt ttgcacttac tcaaacacaa atggcgacat atgagcataa ttttaatgat
     3361 tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc cgtcggatat tattgttgga
     3421 ctttataacg ataccattaa attaaattta cattttgaat ggaccaataa aaataacatc
     3481 acgttgtcaa ataatcagac cagtttcacc aatggttatt cggtaacggt gacacctgcg
     3541 gccagtaatg caaaagtgaa tgtttctgcg gggggcggcg gttcagtgat gattaatggt
     3601 gttgcgacat tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta
     3661 ctgtgtttat taggtggcaa gtcatgggat gcatgtgtaa atagctatag aaatgcattg
     3721 gcacaaaatg caggtgtcta ttcctttaat ctgaccttgt catacaaccc gataaccaca
     3781 acctgcaaac cggacgattt attaattact ttagagggta ttcccgtttc acaattacca
     3841 gccacaggta acaaagcaac aataaatagt aaaaaagggg atattattct gcgttgtaaa
     3901 aatttattag gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct
     3961 gacttgttaa ccaacagcaa cacaatactg aaaggtgcgg aagataatgg cgtaggattt
     4021 attcttgaaa gtaatggttc gccagtcaca cttttaaata tcactgataa cagcaaagga
     4081 tacacaaatt taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt
     4141 ccgataacag ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag
     4201 gcaaccgcat taatcaacgt gaaatacgac taataacaag caagacgagc aagtggctaa
     4261 taataaaaaa taacaaggtg tctgcatttc cccctcatga tgagggggat ttttttagcg
     4321 atgataaaaa atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa
     4381 cacgtacgcg ccacccatat aggtccagtg cgtcccggca tcaggtgcgg gcaggttacg
     4441 taactgccac tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc
     4501 tggtttgaaa tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga
     4561 ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag
     4621 cacacccagc aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat
     4681 atcagtcgca tatctttcct gccatacaat ataactgaca agcgatattt tgttagtgat
     4741 cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag
     4801 agagtcagcg gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct
     4861 aaaggattga gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccaact
     4921 aagccaatca acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc
     4981 cagcgttttg cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gaccagcgtt
     5041 aacgccaggc caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac
     5101 tgaatgcaaa tcaacagcac ggtaattgct gccggacagg ggataagacc acctgttaag
     5161 ccaaataaca aaatttgcca gttggtgacc tctctaccat caaagcgtcg tttaatatca
     5221 ttggcatggg ctcgtgcatg ggcatcctga tactcgccat gttcgtgatg gtgatgatgt
     5281 ccatggtcgt ggtgatcttc gtgatcgtga tgatgatgct cataatcatg cccgtgcata
     5341 ttctccagcc agttgcgttc gccgcgccag gtacgccaga acatccagaa cgcggtgcta
     5401 ataatgatca ctgcggaaat cagctggagc cacggttctg ctgattgagc agtaaagcgc
     5461 ttactgatca ccatcccgcc aaaggcaatt aaccagacca ctgcggtatg cgaaatagtt
     5521 gctgccagtc cgagcatcac cgcttgttta atggtgcctt tgatggcgat gataaacgcc
     5581 gccatcatcg tttttgagtg ccctggttcc aggccatgaa gcgcaccaag taagatggcg
     5641 ctggggatga agaaccaggc gtttccttgc tgaagaagag ttgtacattc ggtcatgata
     5701 ataattctta gtattaattc ggcaatctga ttctactccc ccccagtacc tgatactacc
     5761 ccccagtaga ttaatagtgc tatgattttt catattcttg ttaaccaggt gttgccatgt
     5821 ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggccagg
     5881 tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga
     5941 ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctga
     6001 cggaacacat cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgtcgttc
     6061 tgaaggtgct ggattcatat atcaaataat ttattaacgc gattgtaaaa ctgccgtttt
     6121 tcctcgttta caacgcgtgc gctggacatt accatcctcc tctgcgattt atcatcgcaa
     6181 ccaaacgact cggggtgccc ttctgcgtga aggctgagaa atacccgtat cacctgatct
     6241 ggataatgcc agcgtaggga agtcacggac caccaggtca ttgcttcttc acgttatggc
     6301 aggagcaaac tatgcaagtc gacctgctgg gttcagcgca atctgcgcac gcgttacacc
     6361 tttttcacca acattcccct cttgtgcact gcatgaccaa tgatgtggtg caaaccttta
     6421 ccgccaatac cttgcaggcg ctcggtgcat cgccagcgat ggttatcgaa accgaagagg
     6481 ccagtcagtt tgcggctatc gccagtgcct tgttgattaa cgttggcaca ctgacgcagc
     6541 cacgcgctca ggcgatgcgt gctgccgttg agcaagcaaa aagcgctcaa acaccctgga
     6601 cgcttgatcc agtagcggtg ggtgcgctcg attatcgccg ccatttttgt catgaacttt
     6661 tatcttttaa accggcagcg atacgtggta atgcttcgga aatcatggca ttagctggca
     6721 ttgctaatgg cggacgggga gtggatacca ctgacgccgc agctaacgcg atacccgctg
     6781 cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt cactggcgag atggattatg
     6841 ttaccgatgg acatcgtatc attggtattc acggtggtga tccgttaatg accaaagtgg
     6901 taggaactgg ctgtgcatta tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata
     6961 cgctggaaaa tgtcgcatct gcctgtcact ggatgaaaca agccggagaa cgcgcagtcg
     7021 ccagaagcga ggggccaggc agttttgttc cacatttcct tgatgcgctc tggcaattga
     7081 cgcaggaggt gcaggcatga aacgaattaa cgctctgacg attgccggta ctgatccgag
     7141 tggtggtgcg gggattcagg ccgatcttaa aaccttctcg gcacttggcg cttatggttg
     7201 ctcagttatt actgcactgg tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat
     7261 tgagcctgat tttgtcgccg cccagctcga ttcggtgttc agcgatgtgc gaatcgatac
     7321 cactaaaatc ggtatgctgg cggaaaccga tattgttgaa gcggtggcag aacggttgca
     7381 acgttatcag atccaaaacg tggtactcga caccgttatg ctggcaaaaa gcggcgaccc
     7441 gctgctttca ccttcggcgg ttgctacgct gcgcagtcga ttattgccac aggtttcatt
     7501 aataacgcca aacttgcccg aagctgccgc cttgctcgac gcgccacacg cgcgcaccga
     7561 acaggaaatg ctggaacaag ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat
     7621 gaaaggtggt catctggatg atgagcaaag cccggactgg ctgtttaccc gcgagggtga
     7681 acaacggttt accgcaccgc gcattatgac caaaaacacc cacggcactg gttgtacact
     7741 ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac tgggctgaca ccgtacagga
     7801 ggcaaaaagc tggctttcat cggcgttagc ccaggccgac acgctggaag ttggtcacgg
     7861 tattggtccg gttcaccact tccacgcctg gtggtgagca ccgtactata gctgctttgg
     7921 ttattgtcag cctttggcac tgacattcag ggatgaaacc aatgcagcta agaattacca
     7981 gtcgaaaaaa actcacctct ctattatgcg ctcttgggct aatttccatc gttgcaattt
     8041 atccgcgtca gacggtgaat tttttctatt cgacagcagt tcagattaca gactacatcc
     8101 acttctacgg ttatcgcccg gttaaatctt tcgctattcg cattcctgcc agttacacca
     8161 ttcacggaat agatgtttcg cgctggcagg agcggatcga ctggcagcgg gtggcaaaaa
     8221 tgcgcgacaa cggtatccgc ttacagtttg cttttattaa ggcgacggaa ggcgaaaagc
     8281 tggtggaccc ctatttttcg cgtaactggc aactaagccg cgaaaatggc ctgctgcgtg
     8341 gggcgtatca ttatttctcc ccgtcggtat ccgcttcagt tcaggcgaga ttatttctgc
     8401 aaacggtgga tttctcacaa ggcgatttcc ctgccgtgct ggacgtagaa gaacggggaa
     8461 aattatcggc aaaagaatta cgcaagcggg taagtcagtg gctaaaaatg gtcgaaaaaa
     8521 gtacggggaa aaagccgatt atttactcag gagccgtttt ttatcacacc aatctggcgg
     8581 gctatttcaa tgaatatccg tggtgggtgg ctcactacta tcaacgtcgt ccggacaatg
     8641 acggaatggc ctggcgcttc tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg
     8701 gtccggtgga ttttaatgta tttaatggca cggtggagga gttgcaggca ttcgttgatg
     8761 ggattaaaga aacgccttaa ataagaagca gtgatatact cccaccagac tccccggcag
     8821 gaaaaagaca aatggaacaa gcgcataccc agctgattgc ccaactgaac gagcgtattt
     8881 tagcggcgga taacacgccg ctttatatta agtttgccga aacggtaaaa aatgccgtgc
     8941 gcagcggggt gctggagcat ggcaatattt tgcccggtga gcgtgattta agtcagttaa
     9001 ccggcgtttc gcgcattacg gtgcgcaagg cgatgcaggc gctggaagaa gagggtgtgg
     9061 tgacgcgttc gcgcggttac ggcacgcaga tcaacaacat cttcgaatac tcgttgaaag
     9121 aagcgcgagg tttttctcag caggtggtat tgcgcgggaa aaagcccgat acgctatggg
     9181 ttaacaagcg tgtcgtgaaa tgtcccgaag aagtcgcgca gcagctggcg gtcgaagcag
     9241 gaagtgatgt ctttttgctt aagcgtattc gctatgtcga tgaagaagcg gtatcgattg
     9301 aggaatcgtg ggttccggcg catttaattc atgatgttga tgccatcggg atttcacttt
     9361 atgactattt ccgcagccag cacatttacc cacaacgtac gcgttcccgc gttagcgccc
     9421 ggatgccaga tgccgagttt cagtcacata ttcagttaga tagcaaaata ccggtgctgg
     9481 tgatcaagca agtggcgctt gaccaacagc aacggcctat tgaatacagc attagccact
     9541 gtcgcagcga tctatacgtt tttgtgtgcg aggagtagtt cctcgcgcgt tggcgcacaa
     9601 tcaccgcccc gatgcccgac aacccacgac gccactgcat tgcccagtaa tacggcatcc
     9661 gccagtggca gaccagaggc cagcccggca agtacgccac cggcatgact gtcgcccgcc
     9721 ccaatggtgt cgacaacttg cgttggaaat gccggaatgc agccagaagc gtcgttgctg
     9781 aaataccatg cgccttcttt atcgagacga acgatcaacg gcgcggcaaa tttctcctgc
     9841 cattgtttgc caagtgttgt tatctctgcg gataaagcaa aacgttcggc ggcaatctca
     9901 gcctcttgac gattgagcga cactaaaggt cgacaggcca tgatccgcgc cagtaatgca
     9961 tccgggatat cgccaatacg tgggccaaaa tcgataaacg gcgtcacgtc ttgcagcttt
    10021 tccagccatt ccactaataa ttcgccgcag ggcgaggcca gttgataacc ggaaaaatag
    10081 agcaggctgc caggcgcaac ggttaatcgc gccagacact ggcgattcca ctgattttcc
    10141 acaccgctga atgacataaa agtgcgttcg ccatccggct caaccagcgc cagacaccag
    10201 ccgttatcac cttcggcgtt atcgatcagg ctgattaagc cctcttttgc catccggttg
    10261 cgaataatct ccgcccacac gccctgaccg agcggcaagg cattacccgc ttcgatgccg
    10321 aggcgcttta acgccacggc aatattcagt gcgcagccgc caacgttaac gctctgctgt
    10381 ttcagttcga tatcgcaccc acgccagggg agggcataag cgtcggcgat cacgtcaatg
    10441 accgccgcgc cgacgaccat caccgactga cgcgtggtta attcaggcag cagcgtgtgt
    10501 aatcgagcgc cgctcatacc gcctcccgtt gttgacgata tttcgccagc gctgtggcat
    10561 agcggttgaa atcaagctga tttaccgcat ccagctccgc ctttaacgca ggatcgatag
    10621 cgttaatgcc atgcaacgcg ccgcagattg ccgtcgctat agcaccgatg gtgtctgtat
    10681 cgccgccaag gttagcgcac aggacggcgc aacggttcgg gtcggtttgt gccagttcaa
    10741 ccagcgcaat ggcgcacgga acggactcaa tagtgctggt acctgcgcca acgacctggt
    10801 aaagctgttc gctggcggat tcggtgccgt cggcattgcg cacaattttc agcgcaatct
    10861 ccaaccgcgc tgccagtgag gcgctgaacg tagtgatgcg tttttgctgt gcatgtcgcg
    10921 caattgaagg cagggaatca acaatcgctg accagctttc tccgtcaatg gcacgagaaa
    10981 tcgcccatgc gatgactacc gcgcctgcaa ccgccagatc ggatttatgg gtcgggctgg
    11041 acgccagcgc tacatcatca ataaaggaat caacatcacg cgccggaagc aaacaaccta
    11101 atggcgagac gcgcatcgct gcgccgttgg tcacgccgtt gttttccagt tcagcaacgg
    11161 gtttaccgtc gcgaatggcg ttaagcgcaa tcttcgaggt cggacctagt acgtttttgt
    11221 taaaggcgtc gaaacgcagc gcccagtcga gaatattacg cccaatcaga tccggatcga
    11281 tcttgccttc acgttccagt aacgcatccg ccagacacag cgccatcgag gtatcgtcgg
    11341 tgaattcggc gcggttaaaa taacaggccg cgttattctc ctttggtcca ggaagaaaac
    11401 ggtcaatcca gccaaagtgt gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc
    11461 ccatcgcatc ccctaacgcc tgcccataaa gagcaccgag aatacgttct gttttcattt
    11521 aacttcccct tgtgtcaacg caatatcgcg atcatcgacc ttgatagccg taatttcgtt
    11581 gtcggattcg cggaaaaaaa tcatgaacag cacggcgata atggcaatca tcaccgcgcc
    11641 gaaagtccac atcccggacc agttgaaagt cagtccgttt accggttcct gataagcgaa
    11701 catcttttcc atcatcacac cgccaagacg atagccgagc aaactgccga agccctggca
    11761 gcagagcgtg atcagcccct gcgcagcggt acgcatatgc acgggggctt ttttatcgac
    11821 atagatgtaa gcggtaacgt agtaaaaatc gtaacttacg ccgtgaagca aaataccgag
    11881 gaacagtaac gcgtaggtga aatattcatc cgcactaccg taaataaaga agccatagcg
    11941 gatcgcagcg gtgaccagac caagcaataa tacctttttg ataccaaagc gtttagtgaa
    12001 aaacggcaat gccagcataa agaagatttc agagaactgg ccgagcgtca tccagccggt
    12061 gacgtttttc atgccaactt cggtcagata accgttggca aagatgtaat agaacgctag
    12121 tggcatcgca aacaggaatg aacagaagaa aaagacgagg aagtttttat cgcgcagcag
    12181 gatcagcgca tccaggccga gcatgacttt aatatccatt ttgccggtgc tttttggtgg
    12241 cgtgtcgggc aggaaaaacg caaacacacc aagcagagca gaacttccgg cggtaatcag
    12301 cagcgggatg ttagtcggtg agatatcggc ataccccagt atttgcggca agaaaccaca
    12361 tgccagaccg gaggcaatcc agccgatagt gcccatcaca cgaatgcgcg ggaaatcacg
    12421 ctcaacatcc ggcacgttgg caaaagcgat gctgttagtc agcgcaatgg tcggcatata
    12481 ggttagcgag taggccagca gtaacgggaa gaacccggca aaagtggtct gttgcgcagc
    12541 gaaatacatc agcagcgcgc ctgcgaacat caataccgcc agcactttct gcgccgagaa
    12601 aaagcggtca gtgatggagc caaccagaat tggcgacagg atcgccgcaa tggcggtaca
    12661 ggcatacgac cagccaattt ctccggcact aaaaccgctt ttacttaacc acaaccacaa
    12721 tggcacaaac cacgcgcccc agataaacca ttcaacaaac atcatgaacg acagctttgc
    12781 tgttgttttc atcttttaat tccttcatga caggtaatgt aaggtacgcg atgacaatac
    12841 cacttaataa taccttttaa atacctttgc ggtgaaaact tgaccgcgct aacatttttt
    12901 ctgatgaatc gagccaacag aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc
    12961 cgtaacgacc aggattatga ttgcccgcaa aaggcgggcc aggaaaatcc atagccgata
    13021 tccaatcgga atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga
    13081 cgccgacaac cttttacagc accgttgtat gactattcct tctgaccagc tttatctccc
    13141 cggacatgac tacgtagacc gcgtgatgat tgacaataat cgcccgcctg cggtgttacg
    13201 taatatgcag acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct
    13261 gccggttgac cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta
    13321 ctttgacccg aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcgtc
    13381 aacttacggc gtgctggcgt cggtatcgcg gcgttatgcg catcgcattc cattcctcgt
    13441 caaacttaat cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc
    13501 cagcgtggag caggcgttca acatgggcgc ggttgcggtt ggtgcgacta tctattttgg
    13561 ctcggaagag tcacgtcgcc agattgaaga aatttctgcg gcttttgaac gtgcgcacga
    13621 gctgggcatg gtgacagtgc tgtgggccta tttgcgtaac tccgccttta agaaagatgg
    13681 cgttgattac catgtttccg ccgacctgac tggtcaggcg aaccatctgg cggcgaccat
    13741 tggcgcggac atcgtcaaac agaaaatggc ggaaaataac ggcggttata aagcgattaa
    13801 ttacggttat accgacgatc gcgtgtacag caagttaacc agcgaaaacc cgattgatct
    13861 ggtgcgttat cagttagcta actgctatat gggccgggct gggttgataa actccggcgg
    13921 tgctgcgggc ggtgaaactg acctcagtga tgcagtgcgt actgcggtta tcaacaaacg
    13981 cgctggcgga atggggctga ttcttggacg taaagcgttc aagaaatcga tggctgacgg
    14041 cgtgaaactg attaacgccg tgcaggacgt ttatctcgat agcaaaatta ctatcgcctg
    14101 atacgcctta tacgaacaac accaccccgt aggacggata aggcgtttac gctgcatccg
    14161 gcaacctatg cctgatgcga cgctgaagcg tcttatcatg cctacatagc actgccacgt
    14221 atgtttacac cgcatccggc ataaaaacac gcgcactttg ctacggcttc cctatcggga
    14281 ggccgttttt tgcctttcac tcctcgaaca attttcatat tgtcgttttt gtgatcgtta
    14341 tctcgatatt taaaaacaaa taatttcatt atattttgaa atcgaaaaca aacgacaggg
    14401 tatgaaaatg tacgtggtat cgacaaagca gatgctgaac aacgcacagc gcggcggtta
    14461 tgcggttccg gcattcaata ttcacaatct cgaaacgatg caagtggtgg tagaaaccgc
    14521 tgccaacctg catgcgccgg tcatcatcgc cggaacgcct ggcacattta ctcatgctgg
    14581 tacagaaaat ctgttggcgc tggtcagcgc gatggcgaag caatatcacc atccactggc
    14641 aattcatctc gaccatcaca cgaaatttga cgatatcgct cagaaggttc gttctggcgt
    14701 gcgctcagtc atgattgacg cctcgcattt gccttttgcg caaaatattt cacgggtcaa
    14761 agaggtggtg gatttttgcc atcgctttga tgtcagcgtc gaggcggagc tggggcaact
    14821 tggcggccag gaagatgatg tgcaagtcaa tgaagccgat gcgttgtaca ccaaccccgc
    14881 tcaggcgcgt gaatttgccg aggcaaccgg aattgattcc ctggcggtcg ccatcggcac
    14941 ggctcatggg atgtatgcca gcgcaccggc gcttgatttt tctagactgg agaacattcg
    15001 ccagtgggtg aacttaccgc tggtgctgca tggcgcgtca gggttatcga ctaaggatat
    15061 tcagcaaacc atcaaactgg ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc
    15121 cttctcgcag gcgttaaaaa attacctgac cgagcaccct gaagcgaccg atccccggga
    15181 ttatttgcag tcggctaaat ccgcaatgcg cgatgtggtg agcaaagtga ttgccgattg
    15241 tggctgcgag ggcagggcat aacgcacctg ccatttaaca aggaaaaaac atgaaaacgt
    15301 taattgcccg gcataaagct ggtgaacata tcggcatatg ttcagtctgt tctgcccatc
    15361 cgttggttat cgaagcggcg ctggcatttg atcgcaacag cacgcgcaaa gtgctgattg
    15421 aagcaacgtc aaaccaggtc aatcaatttg gcggttatac cggaatgaca ccggcagact
    15481 ttcgcgaatt tgtttttacg attgccgata aagttgggtt tgcacgcgaa cgcattattc
    15541 tcggcggcga tcatctgggg ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg
    15601 aaaaatccgt cgagatggta aaggaatatg ttcgtgccgg cttcagtaaa attcatcttg
    15661 atgcgtcaat gtcctgcgcg ggggatccca taccgttagc accagaaacg gttgcggaac
    15721 gagctgctgt gctttgcttt gctgcggaaa gtgtggcgac agattgccag cgtgagcaac
    15781 tgagctatgt cattggcacc gaagttccgg ttccgggcgg tgaggccagc gccattcagt
    15841 cagtacacat cacccatgtt gaagatgccg ccaatacttt acgtacgcat caaaaggcct
    15901 ttattgcccg tgggctgaca gaggcgttaa cacgtgtgat tgccatcgtg gtgcagccgg
    15961 gtgtggaatt tgatcacagc aatattatcc attatcagcc gcaggaagcg cagccgctgg
    16021 cgcaatggat agaaaacacc cgaatggttt atgaagcaca ttctaccgat taccagaccc
    16081 ggacggctta ttgggaatta gtccgcgatc actttgcaat attgaaagtc ggtcccgcat
    16141 taacctttgc tttacgcgag gcgatatttg cactggcaca aattgagcag gaacttatcg
    16201 cccctgaaaa tcgcagcggt tgcctggcgg taattgaaga agtgatgctc gacgaaccgc
    16261 aatactggaa aaaatattat cgtacgggtt ttaacgattc attactggat attcgttaca
    16321 gcctgtcgga tcgtattcgt tattactggc cgcatagtcg gattaaaaat agcgtcgaaa
    16381 cgatgatggt gaatcttgaa ggcgtggaca tcccactggg catgattagt cagtatcttc
    16441 ccaaacaatt tgaacgcatt cagtccgggg aattatcagc aataccgcat cagctgatta
    16501 tggataaaat ttatgatgtt ttgcgcgcct atcgctacgg ctgtgcggaa taaggacggt
    16561 atatgactaa cctgtttgtt cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa
    16621 cccatatcgg taatgagatg ctcgccaaag gtgtggttca tgatacctgg ccacaggcat
    16681 taattgccag agaagcagaa ttccctaccg ggataatgct tgagcagcac gctattgcaa
    16741 taccgcattg tgaggcgatt catgctaagt cgtcagccat ttatctgtta aggccaacaa
    16801 ataaagttca ttttcagcaa gcggatgatg ataacgacgt ggcggtatcg ttggttattg
    16861 cgttgattgt ggaaaatccg cagcagcaat tgaaactttt acgctgttta tttggcaagt
    16921 tacaacagcc cgatatcgtc gagacactaa tcactcttcc tgaaacccag ttaaaggaat
    16981 acttcacaaa gtatgtttta gattcagacg aataaatccc tctgtaacaa taaaaaggac
    17041 tatttatgaa acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg
    17101 cggcggaaga aattaaagag ttttgtcaga atcataatat tcctgttgaa ttaatccagt
    17161 gtcgggttaa tgaaatagaa acctatatgg atggtgtgca tttgatatgc accactgcca
    17221 aagtggatcg tagttttggc gatattccgt tagttcacgg catgcctttt atttctggta
    17281 tcggtatcga agcattacaa aataaaattc tgactatctt acaggggtga cctatgtttt
    17341 cagaagtcat gcgttatatt ctcgacctcg gccctacggt gatgctgccg attgtcatca
    17401 ttattttttc taaaatatta ggcatgaagg caggcgattg ctttaaagcg ggtctgcata
    17461 tcgggattgg ctttgttggc attggccttg tgattggctt aatgctggat tccattggcc
    17521 cggcggcgaa agcgatggcg gaaaatttcg acctgaatct gcatgtggtc gatgttggct
    17581 ggccgggctc ttcaccaatg acctgggcgt cgcaaattgc gctggtggcg attccgattg
    17641 cgattctggt taacgtggcg atgttactga cccgtatgac gcgggtggta aatgttgata
    17701 tctggaatat ctggcatatg accttcaccg gcgcgttgct gcatctggca accggttcat
    17761 ggatgatagg gatggcaggt gtggtaattc acgcggcgtt tgtttataag ctcggcgact
    17821 ggtttgcccg cgatacccga aatttctttg agctggaagg tattgctatt ccgcacggta
    17881 cgtcggcgta tatggggccg attgcggtgc tggtcgatgc tatcatcgag aaaatcccag
    17941 gcgttaaccg aattaaattt agcgccgacg atattcagcg caaatttggt ccatttggcg
    18001 agcctgtcac cgtgggtttt gtgatggggc tgattatcgg catcctcgcg ggttacgatg
    18061 tcaaaggtgt attgcagctg gcggtaaaaa cggcggcagt gatgctgcta atgccacggg
    18121 tgattaaacc catcatggat ggtttaacgc ccatcgctaa gcaggctcgt agtcgtttac
    18181 aggcgaagtt cggcggtcag gagttcctga ttggccttga tccggcgttg ctgctgggac
    18241 atacggcggt ggtatcggca agcctgattt ttatcccact caccatttta attgctgttt
    18301 gtgtgccggg taatcaggtg ctgccgtttg gcgatcttgc caccatcggc ttcttcgtgg
    18361 cgatggcggt cgccgtgcat cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca
    18421 ttatgagcat caccctgtgg atcgcgacgc aaactattgg tttgcacacc caactggcgg
    18481 ctaatgctgg ggcgttaaaa gccgggggta tggtggcttc aatggatcag ggcggttctc
    18541 ccattacctg gttactgatt caggttttct ccccgcaaaa tattcccggt ttcattatta
    18601 tcggtgcaat ttatctgacc ggtattttca tgacctggcg tagagcgcgt ggctttatta
    18661 aacaagagaa agtcgttctc gcagaataat ttttacctga gggggaggtt atccccctta
    18721 acaatcagga gtttttatga aatcagtggt gaatgatact gatggtatcg tgcgcgttgc
    18781 agaaagcgtc attcctgaaa ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc
    18841 gggcttatgt ggttccgatt tacccaggat atttaaaaat ggtgcacatt attatccaat
    18901 aacgttaggc catgaattta gcggctatat tgatgcggtg ggatccggtg ttgatgattt
    18961 acaccctggc gatgcggttg cctgtgtgcc gttattaccc tgttttactt gtccagagtg
    19021 tctgaaaggg ttttattccc agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg
    19081 tggatttgct gaatatattg tcgttaagcg aaaaaatgtc tttgctctac ccacggatat
    19141 gcctattgag gatggggctt ttattgagcc gattaccgtt ggtctgcatg cttttcattt
    19201 agcgcaaggt tgtgagaata aaaacgttat tattattggt gccggaacca ttggcctgct
    19261 ggccattcag tgcgctgtcg cgctgggagc aaagagtgtg acggcgatcg acattagttc
    19321 agaaaaactg gcactggcaa aatctttcgg tgcgatgcaa acatttaaca gtagcgaaat
    19381 gagcgcgccg caaatgcaga gcgttttacg cgaactgcgc tttaatcagc ttatcctcga
    19441 gacggctggc gtaccgcaaa ctgtcgaact ggcggtagag attgccggtc ctcatgccca
    19501 actggcgctg gtgggcacgt tgcatcagga tctgcattta acatcggcaa cgtttggcaa
    19561 aatatagcgt aaagagctga cggttatcgg cagttggatg aactactcca gcccttggcc
    19621 ggggcaggag tgggaaacgg cgagccggtt gctgacagaa cgtaagttaa gcctggagcc
    19681 attaatcgct caccgtggaa gctttgaaag cttcgcccag gcggtgcgtg acatcgctcg
    19741 taatgctatg ccgggcaaag tgttgctcat tccctgaaac cgcgggccag cgtgatgctg
    19801 gcccggtatt gtgcaaaaca gatcattcac caatggtccc ccttcgttta cactagccac
    19861 aattgaatat gggtaaatga cgatgaattc attcgagcga aggaataaga tcatccaatt
    19921 agtgaatgaa cagggaaccg tgcttgttca ggatctggcg ggagtatttg ctgcctcgga
    19981 agcgacaatc cgtgccgatt tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca
    20041 tggcggtgcg gcgaaaataa tgtctggtaa tagtgaaacc gagacccagg aagtcgggtt
    20101 taaagagcga tttcagctcg ccagcgcgcc aaaaaacaga atagcgcagg cggcagtcaa
    20161 aatgatccac gaagggatga ccgttattct cgacagcgga agtacgacaa tgcttatcgc
    20221 cgaaggatta atgaccgcca aaaatatcac ggtgattacc aacagtctcc cggcagcgtt
    20281 tgccctttcc gaaaataaag acattactct ggtcgtctgt ggtggcacgg tccgccataa
    20341 aacgcgctcg atgcatggtt ccattgccga gcgttcatta caggatatta atgccgattt
    20401 aatgtttgtc ggtgctgatg gtattgatgc ggttaatggt attacgacct ttaatgaagg
    20461 ttattctatt agcggtgcga tggtcacagc cgctaataaa gtgattgccg ttctcgattc
    20521 atcgaaattt aaccgtcgcg gttttaatca ggtactacca atcgaaaaaa ttgacattat
    20581 tattaccgat gatgctgtgt cagaggtgga taaactggca ttgcagaaga cgcgggtaaa
    20641 attaattacg gtgtagtatt gttctgtatg ccggataagg tgtttacacc gcatccggca
    20701 ttacggattc tccctggttc aagagagaat agtttgatta acgcaacagt ggacaatctg
    20761 gtggtaatcg acaacgcaac gctgccggaa gtatttcaat atgaaaattt tgcccactca
    20821 acggttcgcc atcaagatta aaggtgatgt cgtgtggtgc ctgaatatca aaccacgacg
    20881 aagcgccttc gataatattc gggttatctt cgtcagattt taaggttgat acgagagccg
    20941 gaagtatttc atcgccggta aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt
    21001 tcggacacaa ttgctgaccg ccaccggcct gacgcccgtt accaatacca atgaccaggg
    21061 cgtcaccttg ccagtgaaag ttttcaccgc ggatttcaca acggtccggt tgcagagtat
    21121 ccatacgcat taagccatga atgatgtaag agacgctacc cagcgcggct tttaattttt
    21181 ccggcgtttc tgtggtaata cgcgtcccaa atccgcctgt cgccatatta ataaaacagg
    21241 tttgtttgtt gacctgcgcc atatctatcg caatggcgtc accggcaatt gccagtttca
    21301 gcgccttatc cagtgcctca ggaatcccta cactggtggc aaaatcattg gcggttccta
    21361 atggcaaaat tcccagcgcg ggtatgtcat ccccctcaca ctgaatcaac gccgtagaaa
    21421 cttcattaat ggtgccatcg ccaccaccgg caatcaccgt tgcgacgccc aacttccggg
    21481 cctcctctac atatcgtgcg gcatcgcctt tctcccaggt gacccgcaca tggatcgtca
    21541 ttccttcctc acgcaacagc ataattgctt cgcgcaaggg taaattgtca gtacttttgc
    21601 cattaagaat cagtaagctg gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg
    21661 gtctgtagat agtgtagagc agaaaacaaa aggtggagtc agaacaggat gaaagtcgga
    21721 ggataaaaag tcagttcaag tatggaggat tcagtggtta ccaccaggag cttgcaatgg
    21781 ggacagcgaa tactaacggc tgccaccgat aaatttcaaa aaagagcata tacctaatat
    21841 tcaactaaac agtggcatct tcaatataat atattaaagc ccccatggag ttaccctgaa
    21901 gggcctcaat gtccgtaatt ccta
//
LOCUS       DAZUGK010000065        21901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZUGK010000065 DAZUGK010000000
VERSION     DAZUGK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.065"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..609
                     /locus_tag="ACHY5W_002130"
     CDS             <1..609
                     /locus_tag="ACHY5W_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447024.1"
                     /translation="FLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKRSSRSF
                     IRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGPTLVTR
                     DAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQERVQTMSDAKV
                     RHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            732..1358
                     /locus_tag="ACHY5W_002131"
     CDS             732..1358
                     /locus_tag="ACHY5W_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447025.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1397..1642)
                     /gene="zapB"
                     /locus_tag="ACHY5W_002132"
     CDS             complement(1397..1642)
                     /gene="zapB"
                     /locus_tag="ACHY5W_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFS4447026.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2068..2913
                     /gene="glpF"
                     /locus_tag="ACHY5W_002133"
     CDS             2068..2913
                     /gene="glpF"
                     /locus_tag="ACHY5W_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFS4447027.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2936..4444
                     /gene="glpK"
                     /locus_tag="ACHY5W_002134"
     CDS             2936..4444
                     /gene="glpK"
                     /locus_tag="ACHY5W_002134"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4447028.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4674..5684
                     /gene="glpX"
                     /locus_tag="ACHY5W_002135"
     CDS             4674..5684
                     /gene="glpX"
                     /locus_tag="ACHY5W_002135"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS4447029.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVSAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEIQVH
                     IL"
     gene            5781..6527
                     /gene="fpr"
                     /locus_tag="ACHY5W_002136"
     CDS             5781..6527
                     /gene="fpr"
                     /locus_tag="ACHY5W_002136"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFS4447030.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6532..6960)
                     /gene="uspD"
                     /locus_tag="ACHY5W_002137"
     CDS             complement(6532..6960)
                     /gene="uspD"
                     /locus_tag="ACHY5W_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFS4447031.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6987..7286)
                     /gene="yiiS"
                     /locus_tag="ACHY5W_002138"
     CDS             complement(6987..7286)
                     /gene="yiiS"
                     /locus_tag="ACHY5W_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFS4447032.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCISKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7498..7938)
                     /gene="yiiR"
                     /locus_tag="ACHY5W_002139"
     CDS             complement(7498..7938)
                     /gene="yiiR"
                     /locus_tag="ACHY5W_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4447033.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8039..8638
                     /gene="yiiQ"
                     /locus_tag="ACHY5W_002140"
     CDS             8039..8638
                     /gene="yiiQ"
                     /locus_tag="ACHY5W_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFS4447034.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8746..9513
                     /gene="tpiA"
                     /locus_tag="ACHY5W_002141"
     CDS             8746..9513
                     /gene="tpiA"
                     /locus_tag="ACHY5W_002141"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4447035.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9568..10323)
                     /gene="cdh"
                     /locus_tag="ACHY5W_002142"
     CDS             complement(9568..10323)
                     /gene="cdh"
                     /locus_tag="ACHY5W_002142"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFS4447036.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10430..11419)
                     /gene="sbp"
                     /locus_tag="ACHY5W_002143"
     CDS             complement(10430..11419)
                     /gene="sbp"
                     /locus_tag="ACHY5W_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFS4447037.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11738..12700)
                     /gene="pfkA"
                     /locus_tag="ACHY5W_002144"
     CDS             complement(11738..12700)
                     /gene="pfkA"
                     /locus_tag="ACHY5W_002144"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4447038.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12881..13783)
                     /gene="fieF"
                     /locus_tag="ACHY5W_002145"
     CDS             complement(12881..13783)
                     /gene="fieF"
                     /locus_tag="ACHY5W_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFS4447039.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(14019..14237)
                     /gene="ogrK"
                     /locus_tag="ACHY5W_002146"
     CDS             complement(14019..14237)
                     /gene="ogrK"
                     /locus_tag="ACHY5W_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFS4447040.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(14318..15481)
                     /locus_tag="ACHY5W_002147"
     CDS             complement(14318..15481)
                     /locus_tag="ACHY5W_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFS4447041.1"
                     /translation="MLDTLTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKVGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKAPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDD"
     gene            complement(15481..15960)
                     /locus_tag="ACHY5W_002148"
     CDS             complement(15481..15960)
                     /locus_tag="ACHY5W_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4447042.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     TGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(15975..18422)
                     /locus_tag="ACHY5W_002149"
     CDS             complement(15975..18422)
                     /locus_tag="ACHY5W_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4447043.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKSRISETTVQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAVFTTVGSAVMTAIGAVSWPVVAVVAAIVAGALLIRKYWEPVSALFG
                     GVVEGLKVAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDALDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTVPAGRSYVDQSKNEYHINLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(18415..18570)
                     /locus_tag="ACHY5W_002150"
     CDS             complement(18415..18570)
                     /locus_tag="ACHY5W_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFS4447044.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(18567..18842)
                     /locus_tag="ACHY5W_002151"
     CDS             complement(18567..18842)
                     /locus_tag="ACHY5W_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFS4447045.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(18899..19417)
                     /locus_tag="ACHY5W_002152"
     CDS             complement(18899..19417)
                     /locus_tag="ACHY5W_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFS4447046.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(19430..20620)
                     /locus_tag="ACHY5W_002153"
     CDS             complement(19430..20620)
                     /locus_tag="ACHY5W_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFS4447047.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAEIFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSITATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDQLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            20939..>21901
                     /locus_tag="ACHY5W_002154"
     CDS             20939..>21901
                     /locus_tag="ACHY5W_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HFS4447048.1"
                     /translation="MKPEQMKNREELTSFATKLATQIKMQTSSNLNDLSTAQESSLLP
                     LINHAWGEQFEDMNRISQNYPGLDYVQPGKRLGLQMTATARKVKYQKTLDKLRNNEKL
                     KGKFDEIWFFVLTAERLPDNVREHPDDFQCKYFTLYDLVEKVMGQTLEFQRDFLTLAR
                     KEYSDYFSPGDVAFNQGYCVSENKPIPFDLSLFNALIHTNEWFDDVDEGEQIVYEFLE
                     LFKNRLRQCTLSARNLLSKIISEINIPKSTSTKIEFPEKQLFGPLMIDDTNYQSFSKD
                     LDDLFEMSLIERWDNYVGMYYKGDDAYIKNERMISVSFPKIEPEM"
BASE COUNT         5341 a         5737 c         5373 g         5450 t
ORIGIN      
        1 tttcttttat ctggcaacgc gggtgaaatg attttatatt ttgctaacgt attttttaac
       61 ccacttgcct tatcatttat tatcgccaca atcatctgta taacgataaa aaaaagaagc
      121 agccgcagct ttattcgcgg cacttgttgg ttaatgggat tatttcttat ctatagcagc
      181 tatacggtgt gggaacgaca cagcatatgg gattatactt tcccggagcc aggtatcacc
      241 gtcacggttc cgagcaaaca gtgggttgcc aatatagtaa aacaaggacc aaccctggtg
      301 acccgggatg cccatgctat tttagctatt gttgccttct cacaaaatga actcggtgtg
      361 cattcaatcg aggaacttac cgcgactcaa aacggaactg cagaaatgca tgtttgtgat
      421 gttcagggat ttgcctgcgc ttatcaggaa agagtgcaaa caatgagtga tgccaaagta
      481 agacatgcta ttttcatgac tctactggat aacactaatt tgatacaact ggtcgcagct
      541 atcgacccag attatttgga cgaatatcaa gaagaagtaa tgaaaatgat gctttctgct
      601 cgtcaatgat ctgatgtgca tgttaataat aatggattga tatgagatac gttatcttat
      661 taatactatg gataataact gtttttctta gtcatcttca gcacagtaac ttgcttactg
      721 gagatcctgg gatggttggc gagtatatcg gttcagtact tcttgggccg ttattacttc
      781 ccgtgcttgt gtcaggtatt ctgtgcacat tcactaaaaa aacgcgtaac ttcgccagtt
      841 ttactcgcgg gtgctgttgg gtattgggag tcctgctttt atccaacgtc ggcaatacgt
      901 ttcgtctgtt caccccgtgg cactatacgt ttgagaaggc agctatttcc gtgactgtcc
      961 caaaccgaca ctggaatacg gtttctatta gtactgataa aaccattgat ataagaagtg
     1021 aagataatag cgtctttatt tctgctttcc gcctgcccgc aggacgtagc gccgatgact
     1081 cgctggaaga gttaaagaag atgcagcgag acaatctcaa ggatcaatac aatgaagaaa
     1141 ccttccagtt tcatgattgt aatgccaaac atttcacctg taagtatcag gatgtgttga
     1201 taaattttga tggtcagcag aaaagaacca tcagtgttta tcttgaagac acccccaggg
     1261 ctgtggggat cattgcatta atggagccag atacggcaga taaatatcgc caacaggcga
     1321 tggagattat gttgtctgct aaaaatacag tgaagtaaaa aataaggcgc cttgcggcgc
     1381 ctctgcatgg aagagattaa acctcttcca tgcgacccag cagggcctgc agacgttcct
     1441 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     1501 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     1561 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     1621 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat
     1681 cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt tagcaccgat
     1741 tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac
     1801 ttttcatcct tctcctggtg acataatcca caccaatcga aaatgttaat aaatttgttg
     1861 cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac
     1921 gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta
     1981 acgataagtt tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa
     2041 caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc
     2101 attgctgaat tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca
     2161 ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg
     2221 gtggcaatgg ccatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt
     2281 accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt
     2341 tcacaagttg ccggcgcttt ctgcgctgcg gctttagttt acgggcttta ctacaattta
     2401 tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg
     2461 gctggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt
     2521 gagatggtga ttaccgctat tctgatggga ctgatcctgg cgttaacgga cgatggcaac
     2581 ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc
     2641 gcatctatgg gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa
     2701 gtctttgcct ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct
     2761 tacttcctgg tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac
     2821 cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga aaaggagacc
     2881 acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac
     2941 tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat
     3001 ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa
     3061 accaggctgg gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt
     3121 agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa
     3181 ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat
     3241 tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga
     3301 agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt
     3361 gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcgcgtcgtg gtgaattgct
     3421 gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac
     3481 cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga
     3541 caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc
     3601 cgaagtgtac ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg
     3661 gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc
     3721 gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa
     3781 atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc
     3841 gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa
     3901 gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg
     3961 tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg
     4021 cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatcc gcgcgacgct
     4081 ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat
     4141 ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcaatt
     4201 ccagtccgat attctcggca cccgcgttga gcgcccggaa gttcgcgaag tgactgcatt
     4261 gggtgcggcc tatcttgctg gtctggcggt cggtttctgg cagaacctcg acgagctgca
     4321 agagaaagcg gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta
     4381 ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga
     4441 gtaatgtcag gcacctgaat cgattgcctg atgccgatcg cctgatgcga cgcttacgcg
     4501 tcttatcagg cctacgagcc aatgccattt tgtgtaggcg ggataaggcg ttcacgccgc
     4561 atccggcatc acgcagactc tgaaaccgtg cgggggcaac cccgcacaca tcaataatcc
     4621 cacccttccc ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac
     4681 gagaacttgc catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat
     4741 ggttaggacg cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta
     4801 tgctcaacca ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag
     4861 caccgatgct ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg
     4921 ctgttgatcc gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg
     4981 tgctggcagt aggcgataaa ggctgtttcc tcaacgcgcc agatatgtat atggagaagc
     5041 tgatcgtcgg accgggagcc aaaggcacca ttgatctgaa cctgccgctg gcggataacc
     5101 tgcgcaatgt ctcggcggcg ctcggcaaac cgttgagcga actgacggta acgattctgg
     5161 ctaaaccacg tcacgatgcc gttatcgctg aaatgcagca actcggcgta cgcgtatttg
     5221 ctattccgga tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg
     5281 acgtgctgta cggtattggt ggcgcgccgg aaggcgtggt ttctgcggcg gtgatccgcg
     5341 cattagatgg tgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg
     5401 aagagaatcg tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag
     5461 ccggtaaagt attgcgtctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca
     5521 ccggtattac caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta
     5581 ccgaaacgct gctgatccgc ggcaaatcac gcaccattcg ccgcattcag tctatccact
     5641 atctggatcg caaagacccg gaaattcagg tgcacatcct ctgattgatt tgatcgattg
     5701 agccttccag tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc
     5761 aatcaaaaca ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga
     5821 actggaccga cgccctgttt agtctcaccg ttcacgcccc tgtgcatccg tttaccgccg
     5881 ggcaatttac caagcttggc cttgaaatcg acggcgaacg cgtccaacgc gcctactcct
     5941 atgttaactc gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca
     6001 aattaagccc acgattggcg gcactgaaac caggagatga agtgcaggtg gttagcgaag
     6061 cagctggctt ctttgttctg gatgaagtgc cagattgcga aacgctatgg atgctggcaa
     6121 ccggtacagc gattggccct tatttatcga ttctacaact aggcaaagat ttagatcgct
     6181 tcaaaaatct ggtcctggtg cacgccgcac gttatgccgc cgacttaagc tatttgccac
     6241 tgatgcaaga actggaaaaa cgctacgaag gaaaactgcg cattcagacg gtggtcagtc
     6301 gggaaacggc agcggggtcg ctcaccggac ggataccggc attaattgaa agtggtgaac
     6361 tggaaagcgc gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca
     6421 atccacagat ggtgcgcgat acccaacagt tgctgaaaga gacccggcag atgacgaaac
     6481 atttacgtcg ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc
     6541 gataaacggc acgatgagca aatccgcact caacttattg atcatcccgc gatatgccgg
     6601 catcaaacgg ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt
     6661 ttgcataatt tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt
     6721 cggccattga atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa
     6781 ttgaagaata tcttctgttg cagggaagta gatacccggg tacaactcgc ttaagccatc
     6841 atcaatatga attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt
     6901 caccagtaag gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat
     6961 gtttaactcc ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata
     7021 atcatctcgg cctggcatga gaaggtgaag gccgcatcaa gctcaacttg cccctccaga
     7081 tccttaatct tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc
     7141 tctttcagtt tggtcataaa ggactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac
     7201 ttactgatac agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta
     7261 ctgcatttat ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt
     7321 acgacttaag aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta
     7381 ccattttttt tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat
     7441 ttgcatcgtg gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta
     7501 attacttgat ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt
     7561 ttcgccttgt gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt
     7621 tgggacaaaa cgccctaccg cccattgcca gacacccggt aaaatcgccc agttacccgc
     7681 cagtagcatc cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg
     7741 caagcgctta acggttactg ccgctgttgg ccagagcaag cacacaaggc aaaacgccgc
     7801 ggtctgaata tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa
     7861 ccacaggcct atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa
     7921 ccattgctgt atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt
     7981 tgacaagggc gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat
     8041 gaagccaggg tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc
     8101 gcatgcgcaa acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac
     8161 tttcgatctc tccatcagcc agtttcgaga agactttaac agccagaatc ccagcctgcc
     8221 actgaacgaa tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc
     8281 cagtaaaatt aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat
     8341 caaaagcatt caaatgacct ggctacccat ccaggggcca gagcaaaaag ccgcgaaagc
     8401 gaaagctcag gaatatatgg cagcggtgat ccgcacactc accccattaa tgaccaaaac
     8461 acaaagccag aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac
     8521 cgagacagaa ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt
     8581 cgctgttgaa ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca
     8641 aaaagcaaag cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg
     8701 gggcggccat cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta
     8761 gtgatgggta actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac
     8821 ctgcgtaaag agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg
     8881 tacatcgata tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac
     8941 gtggacctga acctgtccgg cgcattcacc ggtgaaacct ctgccgctat gctgaaagac
     9001 atcggcgcac agtacatcat catcggccac tctgaacgtc gtacttacca caaagaatct
     9061 gacgaactga tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg
     9121 tgcatcggtg aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt
     9181 cagatcgacg cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct
     9241 tacgaacctg tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct
     9301 gttcacaaat tcattcgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg
     9361 atcattcagt acggcggttc tgtaaatgct tctaatgctg cagaactgtt cgctcagccg
     9421 gatatcgacg gcgcgctggt tggtggtgct tctctgaaag ctgacgcctt cgcagtaatc
     9481 gttaaagctg cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc
     9541 ggcacttact ttccttaatt cttcgcctta acgcaaaatc tcacactgat gatcctgaat
     9601 ttcctcggct gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa
     9661 tgagttatca ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc
     9721 aggtacttct tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac
     9781 ccgacgcgcc aggtattcat gcccgcgcaa accacctggc agtggcaacc agcggctgct
     9841 gatgttcgcc agattgttat ccagctgttc gcgcacatca ggacgaatac aagagatatg
     9901 aatatgaaaa tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg
     9961 atcgggaacc ggctggccgt actttttgct cataaaatca cgcgcctgcc aggccaacca
    10021 aaagaagttc ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata
    10081 cgttggcatc aacagatatt gcagtgggcc attaagatct tttaaaacca cgtatccggc
    10141 attgggtttg acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca
    10201 ttcctcaagg acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc
    10261 aataccggca gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt
    10321 catctttttt tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa
    10381 aaaacccggt cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc
    10441 tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc
    10501 cgaactcttc atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg
    10561 ctacctcagc gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg
    10621 agtagagata tttcaggtag gcttccgcca cctctttagt accttttttc tcgaccactt
    10681 tatcgaccac cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga
    10741 atttatcttt ccccagttca ttcgctgcca gcagagcttc gttttcccag gcaatcagta
    10801 catcgccaat tccgcgctcg acaaaagtgt tagtggagcc gcgcgcgcca gaatccagaa
    10861 cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt
    10921 tgtgatgcag cgcgtagccc caggcagcca ggtaattcca gcgcgcgcca ccagagcttt
    10981 tcggattagg cgtgatcact gaaacacccg gtttaatcag atcgttccag tcatggatct
    11041 gcttcggatt gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat
    11101 ccggcagacg tttgatccac tctttatcaa tccgcccgcg ttccgcaatc gcgtccacgt
    11161 cataggccag agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt
    11221 gtttgcctga accaccgtgt gactgacgaa tcaccacgtt atcgccagtt tgctgtttcc
    11281 agtgggcgct gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa
    11341 cgttaagaag ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc
    11401 ctacgcccca cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg
    11461 ccagaaggtt aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat
    11521 gttgggggtt aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt gcgctttatc
    11581 gatgccggat gcggcgcgaa cgccttatcc ggcctacaaa agtttgtaaa ttcaataaat
    11641 tgcagaattc acgtaggcct gataagcgaa gcgcatcagg catttttgct tctgtcatcg
    11701 gtttcaggct aaaggaatct gcctttttcc gaaatcatta atacagtttt ttcgcgcaat
    11761 ccagccagtc gcctttgaac ggacgcttca tgttttcgat agcgtcgatg atgtcgtggt
    11821 gaaccagctg ttcgttctgg atacctacgc aacgaccgcc gtaacctgcc agcagcagat
    11881 cgatagcgta agcgcccata cgggaagcca gaatacggtc gtaaggcacc ggagaaccac
    11941 cgcgctggat gtggcccagc acagttgcgc gggtttcacg accggtttct ttctcgatga
    12001 aatgcgccag ttcgtcaaca tcacacatat gttcggtaat cgccacgatc gcgtgttttt
    12061 tacctttcgc gatacccgct ttgatttcgt ttaccaggtc ttcacggctg aattcaactt
    12121 ccggaaccac aacgaattca cagccaccag caatggccgc agccaacgtc agatcgccac
    12181 aataacggcc catcacttcc accacggaaa tacgctggtg agaagaagag gtgtcacgca
    12241 gacggtcgat cgcttctaca acggtgctca gcgcagtgaa gaaaccgata gtgtagtcag
    12301 tgcctttgat gtcgttgtca atagtgcccg gcaggccgat gcacgggaag cccatttcgg
    12361 tcagacgcat tgcacccatg taggaaccgt caccgccgat aaccaccagc gcgtcgatac
    12421 cacgtttttt caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg aattccggga
    12481 aacgcgcaga accgaggaac gtaccgccac ggttgatcat gtcagaaacg ctgtaacggt
    12541 ctagctgtac catacggtct tcatacagac ccagatagcc gtcataaatg cccattactt
    12601 ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc atgcctggcg
    12661 catcaccgcc gcttgtcaac acaccgattt tcttaatcat gactacctct gaactttgga
    12721 atgcaaaatg aaatctgttg ccggaagtct tcttgcacat cgaagtgatc caacgaatgt
    12781 gcaaatagta taacaatcac ttcctgctga attgattcag gtcaggccaa atggcggtat
    12841 tttatacaca aaatgcgggt ctggctctct tttatactga ttatgaaagc atagaccgtt
    12901 taccctccct gggtacgacg gaacaggggt cctgatggat aatcacatcc gatcccggaa
    12961 aacgccgtaa aatagcctgc tctacctgat ccgccaccat atgtgcctga accaaaggca
    13021 gagagtcttc catttccaaa tgaatctgaa taaagcgggt cggccctgac tgccgcgtgc
    13081 gaagatcgtg agcgccgcta acacccggcc aggaagtcac gatatcaata atttcttgcc
    13141 gttcctcatc aggtaatgcg cgatccagta atgactgtac cgcctcatat cccatgcgta
    13201 acgcgctata taaaatatag atgccgattc ccaatgcaaa cagagcatcg gcgcgatgcc
    13261 agccgtacca ggacaacccc agcgcgagca gaattgcgcc gttcatcata acatcagact
    13321 ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc acccaacgct
    13381 gaaacgagac aaggataatc gtacaaatta gcgccacaat tgtcacgata accccgacgc
    13441 ctggatctgt catcggtgtt ggagatatca gatgttgaat acccgtcaaa aacaggaata
    13501 gtgccgaacc ggagataaac atactttgcg ccagcgccgc gagggactca gctttaccgt
    13561 gaccaaacga gtgattatcg tcggcaggtt gcagggaata tcgcaccacc aataaattcg
    13621 tcaacgacgc gccgatatcc accagcgaat ccaccagcgc ggcgagaata ctcaccgacc
    13681 cggtatacca ccatgcaaaa attttaatca gcaatagcag cgaagccatc gccgtcgcag
    13741 caatcgccgc ccgactgacc agccgtccat aagattgatt cataaatact cccgctatca
    13801 actgacgcta gtataacgga agcaaatcat ctgcaatgca ttaagcagca ggcaaattga
    13861 ggataaaaaa aacccccaca tcatgtgggg gaagacaggg atggtgtcac aaaaaagcac
    13921 ctaacctgat gatataaatg gatttatgtc aaccactgtc cacatagcgt ccacatcgac
    13981 cataaataag cccctcgact gaggggctat ttttgtgatc acatccacat aatttgctgc
    14041 cctgacggca acgggtgcgg cctcacggcg tggacttctc ccggcttcac gatgtatctc
    14101 tgtaccgact cataagtgat gaacgtggcg ctgcaattca cgttctggca ctggtgataa
    14161 cgctcttttg tcgtgtcagt gatatagcgg cttgtacgcg catgtgcggc atgctggcat
    14221 aaaggacaat gaaacatcgc gagcacctct tccggttttg ttaatggtgc cattttattt
    14281 aatttatcct tataaaacaa acagataaaa ctaaacatta atcatcatct tctgtttcgt
    14341 actccacatc agaaagcctg acctcaagct ctaaggacgt cgtgaagccg ctattattca
    14401 gaaaatgtgt caccttagtg attgtccagt cctgctcgtc tatgacgcgc ttaaagcctg
    14461 acactctgac cggtgtttcc gtgtaaatat cagcacgacc agtggccagg ctgatggaga
    14521 actccgcaac gccccgttgc agtttatccc acttcgcctg agcggcacgc atggcctgcg
    14581 cttttgtggc atataccgta gtcagggcaa aaacgttgtc agcttcacct gccatgtatt
    14641 caccttcgcg cgcttccggt gcttttggcg ctttcttctg cgtgaccggt ttcgctttcg
    14701 ggtgctccag tgcgcgcagg tgtttctctt tctttttgcg tttcagtttt accttctgct
    14761 tttgcggctt cgggtctttg gtgtgtaacc actttgccgt tacgccggtg taagctccac
    14821 ggtcagcaat cgcaaaatga tgacggtcgc cgtcgctgcg ggtgatggta atctgcggga
    14881 tttttttacc gctggccgtc accccctgcc ccactttgag aaacagcagt tttcccattt
    14941 ttaccgacac ctcaccgccg ttgcgttcag caaggcgggt caggaatttc gcatcagact
    15001 cctgcgactg gtcgatgtgc ggaattttaa ttccggccag tgacggagcg acactggctt
    15061 ccagcctgtt acgggaggct atcgcctcaa caatcgcacc gagcgtggtg tcatgccatg
    15121 aaccttcacg gcgggaattg agcgtcccgc ggaaatctgc actccgggcg cggatggtga
    15181 ccacatccgg cgcgccccgg tgttcaacct catcaacggt aaatttccct ttgcatacca
    15241 gggcaaaacc tttccagccg atatacaccg tcaggacagc gccacgaacc ggcagtccga
    15301 cctgcccgtc ggcatcgttc agttcaatat caagctggtc agcctcaaag ccccggttat
    15361 ccgtcagagt catgctcatc agacggtcgc tgatattgcc ggtaatatcc ctgctgtcga
    15421 gcatcagcat gtaatccggc gtcagcgtac tgcctgcatc aaatgtcagc gtatccagca
    15481 ttatcccgcc cccgtcatac ccgtgaatct ggtcgccata ctgccagcct taccgatgag
    15541 cgattccgcc tgtttaccga tatcgccata aagcgcggcc agtgattcat caacgcgggt
    15601 gagtgacagc gtaaaatcaa ttttccgggg tgtgccgtct gcaaagaaaa tactccctgt
    15661 ttcactcacc ttgctgatga catacatgcc gtaaatcatg ccggtgccat ccagcaacgg
    15721 ccacgcccgg ccttcctctg ccatcagtct gagcgtggtc atcgtcagct ttccgccggt
    15781 cagttcagga taaagcacac cggcaagcgt gatgttttcc tcacccgtac cgagaaactg
    15841 aaaggcatcc cgtttgccga tacgggaatt tgacggccag cgataatctg attcacgctg
    15901 catggtctgg tgtggcagcg tctggcgcat aaaaacaaac atacctaacg cgagcatcat
    15961 ttttcgttac ctccttaacc gtcatgcatc atgctggcac gggcgcgcgc acgtttatcc
    16021 cgctcgtatt tttcgagcgc atcctgtaac tggcggtcaa gctgtgtccc cggcgcagta
    16081 ccgcccgtca ggttgatgtg atattcgttt ttactctggt ccacataaga gcgaccagcc
    16141 ggtaccgtga ccggctgata agcctgatag cctgcataag agctggtcgc cggaatataa
    16201 ccacctgtgc catacgtggc ggcatgagtt ctggcggcag tctggtcaag tgcgtcagac
    16261 tctttgttga tgacgccgag tttttccagt acccagtcaa taccgctgcg cagtttgttg
    16321 aacgcattaa gcggcagcat cagcgcgtca gccagtgcct gcccgaacat gacgccagta
    16381 tcacggcaac ggttcagggt gtcctgggtg gctttgaccg gggcaatcag gtttttaaac
    16441 cactgccacg cggcctgtaa cttttcgccc agccagtcaa acaccggctt aagtggcgtg
    16501 aacagttccc ccaccggcgc aaatgccact ttcagccctt caaccacacc gccaaataat
    16561 gcgctgacag gctcccagta tttacggata agtaacgccc cggcgacaat tgcggccacc
    16621 acggccacaa ccggccagct aacagccccg atggcggtca taacagcact gccaaccgtc
    16681 gtgaagactg cccccattgc gcctgctgcc gcgatgatgg cattgatgcc ggtgataacc
    16741 ggccaggcta cgaggccaat ggcaccgatg atgccagtca gcgccagcgc gccaccggca
    16801 atgatgccga tggttgacgc cagtgatttg tttttctgga tccagccgtc gagttttaac
    16861 acatactttg tggccgtctg cgtgagctta cgcagcgcgc cttcctgctg gtcaaacagg
    16921 tcagtcccca ctgcctcata agcggactga aactctttaa agtcaccgcc taggttgtcc
    16981 tgcatgatat ttaccagctc ggcggtcttc ccgtctgagg ctttaaacgc agcggtcagt
    17041 ttgtccagct ttccggttga ggcggcagtc atcagcacgg cggcggctga gctggcctcc
    17101 tccccgaaaa tagttttcat gtattcagcc tgctgggcag taccgagccg gtttttctca
    17161 aaactggcct gcatttcttt cagaatggta aatattggcc gggtattccc cttactgtct
    17221 gaggttttca cgccaagctc tttgagtgca tcccatgctt ttcccgtcgg tgcctgcagg
    17281 cgacttaaca cggcacggct tcccgtcccc gccattgagc ctgtgatttt tgcatcatgc
    17341 agcgccccga ccattgcggc ggtttcttca atgctgacac cggcattttt tgccacaggt
    17401 gcggcatagg tcagcgcatc gctcatgccg tcaaaatcgg cggcggtttt gttcatcgtc
    17461 atggagagaa catccccgat atgagcgacc ttatcgtttg aaagctgaaa ggcggatttc
    17521 atccccatca gcagggcggc gttttcttcc atcgtgcggc ggttcgccag cgccatattc
    17581 agcgtgaccg gcgttgccgc ctgaatggca tcaacatccc cacccgcttt cgcgatgatt
    17641 atctgtgcac cggccgcatc atccgccgag gcggcggtgt tgtcaccgag ctggcgcgcc
    17701 tgtttgcgta gtgcggccat ttcggtggag tcttttgcca ctccgagcac agcctgtaat
    17761 tctgagtttt tctgcgcaaa ctcataaccg ggcatcagca gcttaactcc ggccatcgtt
    17821 cccgccgccg caatccccac accggcagcg cccaccgagg ccatatttcc ggccagttct
    17881 tttcctgcct gataacgctg tttgactgcg ttaagttttg cctgttgcgc actgacacgc
    17941 gccagcgcat cacgctgacg gttaagctgt acggtggttt cactgatacg gcttttcagt
    18001 ccctgctcat catgtgcaag attgcgggta ttaattccca cagcggccag ttcccgctgc
    18061 tggcgtttaa cggaatccgt caggcggtta tatttcgcct gtaagtcctc cgccgcacgc
    18121 tttgcggatt ccagcacttt cgcctgagca cgggtcggac gttcggtgtt tttaaactgt
    18181 gtggcaagtg cttcggcttc ctgccgtgcc ttttcaagtg catgaccagt cacggcaagc
    18241 tgtgcgctgg tcttgcgaaa tccctcaata cgggatgcgt gaccattcag ctcgcgcagt
    18301 gatttttgtg tttcccggat atcccccgac agcgacttac tcgctgtgcg gatggattta
    18361 aacgggcggg atgcctggtc aacagccctg agcaatacct gtaattttac attgttactc
    18421 attcgtgttt ccgcttcgcc ggagcgcctt ttcgcgccat gtgatgagtt cggtcaggct
    18481 catgggatac agttctgatg gcggccagtg aaatatcact gccacatccg ccatcaggtc
    18541 atcgaccgag agatttttcg gaaacgtcac tgcaccgagt tcggcgacaa aaaaccgacc
    18601 accttaccgg ccagcgccac aaggtcaggc agttccagcg cggcgacttc ctgctcggtc
    18661 agcatcggtg ccgtcatgcg cggcagcacc ttaatcagtg catcgacttc ggagttcgcg
    18721 accgcagcca gactgacacc gcgcagcgtc ccggcattag gtttcatcag cgtgacctgt
    18781 tcgataacct gctcaccacg cttgaccgga ttgtccaggg taatcacatt ttctttgttc
    18841 atggttttct cacttctgaa tcagggttaa ccggtcagcc tggctgaccg gatgaaaatc
    18901 acaggccgat attgcggcgg tgttgctcca gccggtcgac gccgttcacc ttctcaatca
    18961 tgttgatggt gtcgatttcg accagctcct taccgtccat cgtcagccgg aaataggtgc
    19021 agaccacgga gattttcgac tcggtatctt ctccctgttt cccctcgccg gtgtcgattt
    19081 ctttctgacg tccacgcatg accacctcga cggccaccgt ttcgccggta tcgtcgcgct
    19141 ggtaagaacc tgcaaaacga atcggtacgg catccacacc ggttgcggcg taaagctccc
    19201 agataaccga atccgggaag ccaccgagcg accactccat tgacagcgca tcgtcatcaa
    19261 ggccgaggtc taccggtgcg ctgccattca tccccgcacc gcgatagttt tcgagcttac
    19321 gggtcagttt tggcagcgtg acggacttcg cgacgccctg atagctgtag ccgttcagaa
    19381 agacgttcat taacttgagt ttgcgcggca ttgccatcgg tcaggctcct taattgctgt
    19441 taaccgaggt gaccagattt gccaggtatt tatcggtaat acgctggcgc agggtcaggt
    19501 tttcaagagg aggtaccggt gtatagtcgt agtcgatata cagttttccg gccttgaggg
    19561 tttccgcatc gttggattct tcgctgaacc agcaggtcgc atccacgata tagccgtttg
    19621 ttttcagctc acggaatttg gcattgatgc cgtcaacgat gtcgcgaatc agcgttgcgg
    19681 tgatgggctt gtccaccgcc cacatgtgcg cctcagccat cgtgtcggcc agcacctgcg
    19741 cggtgcgggt gtagttttca aagaggaaca gctggtcatc agagcaggta cggttacccc
    19801 agaagcggaa accgtcgcgg cgaatcagcg tagtgacgcc tgactcgtta agcaggtcag
    19861 catcggtgcc ggactcctgc aaatcccaga atacagatgc gctgatgccg gtaacaccgt
    19921 tcaccccgac attggacagc gttttatgcc agccctgctc ctggtcgatt ttggcacgca
    19981 gccccagtgc acgggcggtt gcatacgcgg tggcggtgat actggtgacc gtatcccatg
    20041 cgaggaaatc cggccagatg accatcagct cacgctggct gaaattctgg cggtaagctt
    20101 tcacctcgga aatggtttta cagccccatg cgctgatata cccgaaagcg cgcaacttct
    20161 gacagactga tgccagtgca accgccacct ctttggtgtc cagtcccggc acaccgagaa
    20221 tacgcggttt aacaccggta accgactccg ccgccagcag ggctttcagt ccggtgtact
    20281 gaccgttttc gtcggtggtg ccgatgatat tggaaacagt ctgcgcaagt ttcgtttctt
    20341 cgtcgtcgcc ggtgccgtct tccacacgca cgactacggt gaccggtttt gactggtcag
    20401 cgatggcctg caacgacgcc gccagcgtgc cttttttacc ggcctttgca attgcgctct
    20461 gcacattggt aatcagcacc ggtttattga gggggaagat ttccgcatcc gcatcgctgg
    20521 ccgtgcagac catgccaaca atggcggtgg atacggtgga aatgacgcgg gtgccgtcgt
    20581 taatctccag cacctgcacg ccgtgatgat agtcactcat ccgtttaact ccgtggttaa
    20641 tgggtgcaac tattttctgt tgtgcagagc ataagacgct atttgacctg actggtcagt
    20701 ggatgaaaca acagattaaa gaaaaggcgg gcaactcgcc cgcccgtcct gatttgtact
    20761 cactcatttt tcaactgaca atttacatag cccaaacgct atcaaatctg acagcctatt
    20821 ttgagcgaaa agcagacatt agcatccttc ttatagaaaa ggtaaacctg cttctatttg
    20881 ttaatcaata gagagtaagt cacaatggca gatacgaata tcggctggag aaattaaatt
    20941 gaaaccagag caaatgaaaa acagggaaga attaactagc tttgcaacga aacttgctac
    21001 tcaaatcaag atgcaaacca gcagcaattt aaatgatctt tctacagccc aagagagctc
    21061 gctattgcct ctaatcaatc atgcctgggg agagcagttt gaggatatga ataggatatc
    21121 ccaaaactac cctggtctcg actatgtgca accgggaaag cgcttaggtt tgcaaatgac
    21181 tgccactgca agaaaggtta agtatcagaa aacattagac aaacttcgaa ataatgagaa
    21241 gttaaagggt aagtttgacg agatttggtt tttcgtcctc acggctgaac gcctacctga
    21301 taacgtacgt gaacaccctg acgattttca gtgcaaatat ttcacattgt acgacctagt
    21361 cgaaaaggta atgggacaga ctttagaatt tcagcgtgat tttttgaccc ttgcccggaa
    21421 agaatactcg gattatttct ctcctgggga cgttgctttc aatcaggggt attgtgtatc
    21481 agaaaacaag ccgatcccct ttgacttgag tcttttcaac gcgttaattc atactaatga
    21541 atggtttgat gatgttgacg aaggtgagca gatcgtatat gaatttctag aactttttaa
    21601 aaatagactg cgtcaatgca ctttatctgc tagaaatctt ttaagcaaaa ttatcagtga
    21661 gataaatata ccgaagagta caagcacgaa aatcgagttt cctgaaaagc aacttttcgg
    21721 gccactaatg attgacgata caaactatca atctttttca aaagaccttg atgatttgtt
    21781 cgagatgagt cttattgaac gatgggacaa ttatgttgga atgtactata agggtgatga
    21841 tgcatatatc aaaaatgaac gtatgatcag cgtgtcattt ccaaagattg aacccgaaat
    21901 g
//
LOCUS       DAZUGK010000066        21675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZUGK010000066 DAZUGK010000000
VERSION     DAZUGK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.066"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..407)
                     /gene="yhaH"
                     /locus_tag="ACHY5W_002155"
     CDS             complement(42..407)
                     /gene="yhaH"
                     /locus_tag="ACHY5W_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4447049.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(690..1676)
                     /gene="yqjG"
                     /locus_tag="ACHY5W_002156"
     CDS             complement(690..1676)
                     /gene="yqjG"
                     /locus_tag="ACHY5W_002156"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFS4447050.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYAEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFS"
     gene            complement(1746..2138)
                     /gene="yqjF"
                     /locus_tag="ACHY5W_002157"
     CDS             complement(1746..2138)
                     /gene="yqjF"
                     /locus_tag="ACHY5W_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS4447051.1"
                     /translation="MKKLEDAGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLTILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2324..2623)
                     /gene="yqjK"
                     /locus_tag="ACHY5W_002158"
     CDS             complement(2324..2623)
                     /gene="yqjK"
                     /locus_tag="ACHY5W_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFS4447052.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2613..3017)
                     /gene="yqjE"
                     /locus_tag="ACHY5W_002159"
     CDS             complement(2613..3017)
                     /gene="yqjE"
                     /locus_tag="ACHY5W_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4447053.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3020..3325)
                     /gene="yqjD"
                     /locus_tag="ACHY5W_002160"
     CDS             complement(3020..3325)
                     /gene="yqjD"
                     /locus_tag="ACHY5W_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFS4447054.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARANEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3363..3731)
                     /gene="yqjC"
                     /locus_tag="ACHY5W_002161"
     CDS             complement(3363..3731)
                     /gene="yqjC"
                     /locus_tag="ACHY5W_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFS4447055.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3878..4261)
                     /gene="mzrA"
                     /locus_tag="ACHY5W_002162"
     CDS             complement(3878..4261)
                     /gene="mzrA"
                     /locus_tag="ACHY5W_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFS4447056.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4265..4927)
                     /gene="yqjA"
                     /locus_tag="ACHY5W_002163"
     CDS             complement(4265..4927)
                     /gene="yqjA"
                     /locus_tag="ACHY5W_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFS4447057.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4949..5032)
                     /gene="yqiM"
                     /locus_tag="ACHY5W_002164"
     CDS             complement(4949..5032)
                     /gene="yqiM"
                     /locus_tag="ACHY5W_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFS4447058.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5272..6048)
                     /gene="exuR"
                     /locus_tag="ACHY5W_002165"
     CDS             complement(5272..6048)
                     /gene="exuR"
                     /locus_tag="ACHY5W_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFS4447059.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6178..7476)
                     /gene="exuT"
                     /locus_tag="ACHY5W_002166"
     CDS             complement(6178..7476)
                     /gene="exuT"
                     /locus_tag="ACHY5W_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFS4447060.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7959..9371
                     /gene="uxaC"
                     /locus_tag="ACHY5W_002167"
     CDS             7959..9371
                     /gene="uxaC"
                     /locus_tag="ACHY5W_002167"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4447061.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRSMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9386..10873
                     /gene="uxaA"
                     /locus_tag="ACHY5W_002168"
     CDS             9386..10873
                     /gene="uxaA"
                     /locus_tag="ACHY5W_002168"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFS4447062.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10956..11507
                     /gene="ygjV"
                     /locus_tag="ACHY5W_002169"
     CDS             10956..11507
                     /gene="ygjV"
                     /locus_tag="ACHY5W_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFS4447063.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11512..12756)
                     /gene="sstT"
                     /locus_tag="ACHY5W_002170"
     CDS             complement(11512..12756)
                     /gene="sstT"
                     /locus_tag="ACHY5W_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4447064.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13312..14277)
                     /gene="alx"
                     /locus_tag="ACHY5W_002171"
     CDS             complement(13312..14277)
                     /gene="alx"
                     /locus_tag="ACHY5W_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFS4447065.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14561..15547)
                     /gene="ygjR"
                     /locus_tag="ACHY5W_002172"
     CDS             complement(14561..15547)
                     /gene="ygjR"
                     /locus_tag="ACHY5W_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4447066.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMN
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(15626..16318)
                     /gene="ygjQ"
                     /locus_tag="ACHY5W_002173"
     CDS             complement(15626..16318)
                     /gene="ygjQ"
                     /locus_tag="ACHY5W_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFS4447067.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVII
                     GPFSEHGFPAQK"
     gene            complement(16395..16898)
                     /gene="ygjP"
                     /locus_tag="ACHY5W_002174"
     CDS             complement(16395..16898)
                     /gene="ygjP"
                     /locus_tag="ACHY5W_002174"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS4447068.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16983..18119
                     /gene="rlmG"
                     /locus_tag="ACHY5W_002175"
     CDS             16983..18119
                     /gene="rlmG"
                     /locus_tag="ACHY5W_002175"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFS4447069.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     TTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFIQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAILCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLNYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18404..18718
                     /gene="higB"
                     /locus_tag="ACHY5W_002176"
     CDS             18404..18718
                     /gene="higB"
                     /locus_tag="ACHY5W_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFS4447070.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18715..19131
                     /gene="higA"
                     /locus_tag="ACHY5W_002177"
     CDS             18715..19131
                     /gene="higA"
                     /locus_tag="ACHY5W_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFS4447071.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(19176..21194)
                     /gene="fadH"
                     /locus_tag="ACHY5W_002178"
     CDS             complement(19176..21194)
                     /gene="fadH"
                     /locus_tag="ACHY5W_002178"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFS4447072.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMLILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNMAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         5411 a         5668 c         5532 g         5064 t
ORIGIN      
        1 taaaagccgg atgagtcaga catccggcga gcagatttgt tttaaggttc cagttttgga
       61 tcaggtccaa agcggttttc gcctggcgta cccgcctggc agttaaagac gatgataata
      121 agccagccga taaacgggat taagaatagc agtgcccacc acgccgagcg gtcggtgtcg
      181 tgcaggcggc ggaactgaac tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc
      241 gtcaggatgc cttcgccacc agcgcgttgc cagcctaaca ttttatccag cagccccagc
      301 acgaacgtaa agatgatgtt gactagaata aacatccagt actctttgcg ccgcgcacga
      361 ccccggaaac cgacataatt ttttagtact ttcagatacc agtccatttt tgcctcaatt
      421 ttgctcaatc acttgttttt aaagcagcaa attaagaata gacgtgaatg aaatggcagt
      481 aaatattatc gcgatgaatt aataaggagc tgtgaaggat aagggcaagc gcaaaattgc
      541 cagatgcgct acgcttatct ggcctacgcg gcctttgcaa catattgaat ataaatgttt
      601 tgtaggtttg ataaggcgtt cacgccgcat ccgacatgaa caacatgcac ttagctaatt
      661 atcagaggca cctcgtaagg tacctttttt taactgaagc gaacatctcg tccatgcggt
      721 tcatcgagat cctgccacgg accaattgaa ataatccccg tagggttgat ggtcttatgg
      781 ctgcggaagt aatgattacg gatgtgatcg aaattgactg tttcggcaat tcccggcatc
      841 tggtagatat cgcgcaggaa gccatacaga ttcaggtaat cgctgatgcg gtgcttatca
      901 cacttgaagt gggtcacata cactggatca aaacggacca gcgtggtcca caggcgaata
      961 tcggcttcgg ttagctggtt gccggtcagg taacgatgct gacctaaaat ctgttccagt
     1021 cgcgccagcg attcaaacac tttttccacc gcctcggcgt aggcttgctg gctggtggca
     1081 aaaccggctt tatacacgcc gttgttaacg gtgtcataaa tccagccgtt aagttcgtca
     1141 attttcgttt gcagggctgg tgggtagtaa tcacccgctt tcgcgcccag cgcatcaaac
     1201 gcggtattaa acatgcggat gatttctgct gattcgttgc tgacgatggt gtggttcttt
     1261 ttgtcccaca gtacgggaac agtaactcgt ccgctgtagt gtggatcggc gtggagataa
     1321 agctgataca gaaattcatg ttgatagagc gtatctccgg ttgctcccgg aaaactgtca
     1381 tcaaaggtcc agccgttttc cagcatcagc gggttcacta cggaaacgga aataaacggt
     1441 tccagtcctt tgagtttgcg catgatcagc gtgcggtgcg cccacgggca ggcgagtgaa
     1501 acatagagat gataacgatc tttctctgcg ataaaaccgc ctgtgccagt ggggccagga
     1561 gcgccatcgg cagtgagcca gttacgaaat gcggaagctg aacgttgaaa tttaccgccg
     1621 gtagatttgg tatcgtacca ggtgtcatgc cagacgccgt caatcagttg acccattttt
     1681 ctctcctcca atagcaaaaa cgaggagctt cctcctcgtt ttttatcatt aagtatagcg
     1741 cgtgcttacc actttttatt cagcaggcgg tcgatgctat acgcgcccgg accggtaatt
     1801 gccagcagca agaatccgcc agaaattgtc aggtttttca tgaacatcag cgagttaacg
     1861 ccttcagcaa agttgctgtg aaataaaaat gctgtcagca gcgtaaagcc cgcagtaaac
     1921 agggcagtgg tgcgagtcag gaaaccgaac aggattgtca gaccaccacc aaactcaagc
     1981 agaatcacca gcggcagcat aaaacccggg acgcccatcg cttccatata ttgttgggta
     2041 cccgcgtaac cagtaatttt tccccagcct gcggtaataa acagaatcgg cattaaaatg
     2101 cgcgctacca gtacaccagc atcttctaat tttttcatca tactctccac tgcgccactc
     2161 aggctgctga ttgttttttt gtgcaagggc gcagtattag cgtcgttgct gtcgatggag
     2221 agaatcataa acgtggtgaa tgatgattgt tagcaaggaa aactgtcaaa aatcttcaaa
     2281 aaatttgatg ggtaaggccg gaatggctcc ggccagaggg aagttaaccg cgaagctgtt
     2341 gctgcttgag ggtcgtttta accagacgcc atgcgctcca tacgccaaaa ccgcgtctgg
     2401 cccagcggac cagcatatta ggatggcgaa tcgtccagat cgccatcacg ctactgccaa
     2461 ccagcgccca ggagcgcaga ctgagcagca tattccagcg acgatcgtaa gcgcctgttg
     2521 cctccagcca ttcacgacga ctggcagaaa gatccagccg ttgttgctgg atctggctaa
     2581 gcagttgcgc cttacgtcgt tcacgttcga ctttactgct cacgggactc ctcctcgagc
     2641 agctgccgat cgtttgccag ctcatggcgt gtatggcgca gcaaggtgga tttacgcgat
     2701 ttacgtaacg tccagatacc gccaatcagt gccagtagca gcaacaccac ggtggtggca
     2761 atcatcgcat tcaggcgata ttgcggatca accgcccaaa taattagcac catcaggctc
     2821 ataagaccaa atgcagcgaa aagcatcgtc aggcccagca tcagtaaaag ttgaaagaga
     2881 ttagcttttt cctcttccag ctccaccacc gccagccgca gacgtgtttc caccatttca
     2941 accatgatgg aaacaattcg ctgcccgatg cccagaacgc ttttaccggg cccttgcgcg
     3001 tgatgagtgt ccgccataat taacgacgcg acagcagaac gccgagcact acaccgattg
     3061 cagcgccaat gcccacgccc gtccatggat tttcacgcac atactcattg gcacgcgccg
     3121 ccgcgacacg ggtttgtttg gcgatggcat caccggtttc acccaggcga taacggctct
     3181 gtttcagtgc ctgctccgct ttgctacgaa tcttactcaa ctcttctttc gacttctcgc
     3241 cagatgagct aagcacctct tccagcgtat cggaaaggga tttcaactca gcacgcagat
     3301 gttccgtagt gtgttctttc gacatagttt tctccaggtg agtaagtagt gactattgtg
     3361 agttagtagt cgcgcgcttc cagctttttc agctcttcct gcgcttctgc cagtttccgt
     3421 tcgcgtttgg caatcttatc ggcatcgcct ttttgcttcg cctcggctaa atcttgctgg
     3481 cgttccgcca cctcatcttt ctgctttgcg attttcttct gatgatcggc acgcagctgg
     3541 ctatctgaac agttggcccg gacttcactc agggctttat tcagaccgtc aatacgattc
     3601 tggttttggt gtttttcggc atagctgatc tccttaagga tattttgctc cttttcctga
     3661 cacagggtag tggcataact accggcacta agagcaaaaa gagaaacagc taaagcgatg
     3721 cggtatttca ttcttgaacc ttccattgtg attcggacct attacaaagg aacgattgtc
     3781 cagacaacga agcggtagtg ttaaccaccg cctcattaat catagacagt tacggtgaaa
     3841 tcaccaaagt gagtgatctg tttcggataa caggaagtta tccgaagcga tgagagttat
     3901 cccgtagccg ggtcagccac tgcatagcct gactgttatt gtcctgctgc gcaatgatgt
     3961 agccttgggg caatgttctg tcgaggaccg cttttgcagc ggcgctctgg tcgctggagt
     4021 caaaggtaat taacagagtg tcgtttttgg gggtgatact tttgaaagga atgccatgag
     4081 cgtcaaggtg atgccagatt gaaaaaccgt ctggcatcgt tgtgccttga tgaacggcac
     4141 gaatcgccag cgtagactct tgctggcgaa ccgcggacca ggccagcaac agcgtgccga
     4201 ccagaagtaa aacagcgccg gaccaggcta gctggcgaag cgacatgcga ggtatttgca
     4261 tcccttaccc ccgatttcca tatttctttt tccataacac gaccagagaa cctgccaggc
     4321 caaaaaccag cagcaccacc gggagcagca tcaggcatga catcagctgg tcctcgtact
     4381 ttaaaaatac cggcgttttg ccgagcatgt aacccagagt tgtcaggatc aatacccaca
     4441 gcagaccgct catccagttg aaaaactgaa agcgcgcgtt attcagccct gataacccgg
     4501 caatcgtcgg cagcagtgtt ctgacaaacg caataaagcg accaattaac agcgccgata
     4561 aaccgtgttt atgaaaaaga tggtgtgcgc gttgatgata atgcgcgggt aaatgagata
     4621 gccagttttg tacggtgcgg gtattgccca gccatcgccc ctgaatatag ctgacccagc
     4681 agccgaggct ggcggcaacg gtcagcagca gaatcgtttg cggatagccc atcgcccctt
     4741 tcgcaatcaa cacgccgacc aataccagta aactgtcgcc cggtaaaaag gccgccggaa
     4801 gcaagccgtt ttcaaggaac aaaattacaa acaagacaaa atacaacatg ccaatcatcg
     4861 atggattggc cagggtttca aaatcctgcg cccacagggc ttgcagcaat tgggtcaaaa
     4921 gttccattca ttattcctgg tgaatcggtt aacgccacgc tggttttaaa agatgcacta
     4981 ccgcaatgtt attgctgttt tggtatgttc tgcgggtttg atacatcaac acagcctgaa
     5041 ctattccctg ttaaatcgtg agataagaaa gcattcaaaa acataaattc aaattgaatc
     5101 taattgtaac aaaggtcaac gttgttcgtt atcaaaatta catgtccgtg gagtctgatt
     5161 ttgctgaatg caggggaggg tggcgagagg atttacacag gctgacactt tatacttttc
     5221 gcttaccgac gagacaaaca ggcgcgtcgc agcgcctgtc ggacaggaat ttcatttact
     5281 gccgctggtg gctgtatcga gatgcaccac cgggttttcg gcgaatagat agcggtcggc
     5341 attgaactcg aagtcgtcgc tggtttcgtt gaataacatg atcttggtgt tttccaggtg
     5401 ctgccacatt gccagcttag cagcatgagg atctttgcga atcagcgcct tgaggatttg
     5461 atcgtggtca tcacaccagt tatcgacggt acgggaatca atgtgttcgt gcagtttttt
     5521 ccagtacggg ttatggctac gctgggtcca cattttttca acgatagccg ccagggcgga
     5581 gttctgcgtc gccagggcta cctgaatatg gaactgcaaa tcccactcgg aatcacggaa
     5641 gcattgttcg ccgcgcgcct gctcctggat ggccatcaat ttcatgatgt cctgtttcgt
     5701 tacctgagtt gccgcgaact cggcaatatt actttcgatg agctggcgag cctggagtaa
     5761 ctcaaacgga ccgtaattgg cgaattccat attattgtcc gccgcctgct gatggcgcgg
     5821 ctggttggaa accacgtgaa tacctgaacc tttacgcact tcgacatagc cttcaacttc
     5881 cagcatgatg atggcttcac gaactaccgt acggctgacg ttcttttcat cggcaataaa
     5941 gcgctcggca ggcagtttat cacccaccag atagacgccc tgttcgatgc gctctttcag
     6001 gtcagcggca agttgttgat acaaacggcg tggttcagtg atttccatat gcgctccagg
     6061 catgatacgg cagactatat ttgttatacc acttttgcga gtttagctct actcgcagtg
     6121 atgaaaaagc cgcctgagaa ggcggctttg aagggaaact aaaacttaca cgaaacgtta
     6181 atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg
     6241 caacacggtc cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag
     6301 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
     6361 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     6421 gtcggaagag agcgtaatca gtgcgccaga cagtgcctgg tgagcaaaac caccgataca
     6481 cagcagcata attgcgacat acgggttggt gaacaaaccg atcatacccg ggccaatcat
     6541 cagcactgca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     6601 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     6661 cggcatccag gcgaacatcg caatctcttt caggttaaag ccgtaaactt taaacatgaa
     6721 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagcgc
     6781 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtact
     6841 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     6901 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     6961 tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgccc ataccaccag
     7021 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtagccta ctgcgatgga
     7081 acgctctttc gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     7141 ttccgcggca ccgaccgcac cacgagcaac agccaggcca ccccagctac ctgccagcgc
     7201 ggttgcacca cagaacacgg cccacaacac agcaaacatt gcataaccga ttttcgtacc
     7261 cagcacatcc agtacataac ccgctaccgg ttgcatgacc gtataagcag cagaataggc
     7321 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     7381 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc
     7441 gatcatatac caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata
     7501 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     7561 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     7621 ccgtaccggt taacccgatt catctaacaa atgagtgaat cggcaatttg cattccgtcg
     7681 acttatgatt tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca
     7741 tcacaaatgt gggaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     7801 cggcctgcag cgagatttac ctctttgaga gtaatttttt taactacgtt tattgatcta
     7861 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     7921 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     7981 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     8041 cgattttcga ttaccattgc catttgccac cgcagcagat tgcggaagac tatcgtttta
     8101 aaaacctgta tgacatctgg ctgaaaggcg atcactacaa atggcgcgct atgcgtacca
     8161 acggtgtggc cgagcgtctg tgtaccggtg atgcgtctga ccgtgaaaaa tttgacgcct
     8221 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg cacctcgaac
     8281 tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     8341 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     8401 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     8461 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     8521 aagcctttaa catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     8581 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     8641 acttcgccgc tcacggctgt aaagtgtctg accacgcgct ggatgtggtg atgtttgctg
     8701 aagcgaacga agcggaactg gacagcattc tggcgcgtcg tctggctggc gaaaccctga
     8761 gcgagcacga agtggcacag ttcaaaactg cggtgctggt gttccttggt gccgaatatg
     8821 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     8881 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt tcgatggcgg
     8941 aagagctgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     9001 tctactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     9061 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     9121 acggtatgga acgtcagatg acccaactgg cgcagctcgg tttgctgagc cgctttgtcg
     9181 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     9241 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     9301 tgctgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     9361 aacttaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     9421 cgcggtcgct ttagcggatt tggctgaagg cacagaagtc agtgtcgata accaaactgt
     9481 taggctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacgaata tcgcaaaagg
     9541 ggccaacgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     9601 ggaacacgtt cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     9661 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg
     9721 tcgcgctaac ggcgatgtcg gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg
     9781 tgtcaacggc atcgcgcggc agatccaaaa tcgtttcctg aaagagacca acaacgccga
     9841 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgctcacagc tgggcgacga
     9901 tcacattaat acccgcacca tgctgcaaaa catggtgcgc cacccgaacg caggcgcagt
     9961 gctggtgatt ggcctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgctggg
    10021 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
    10081 agccggaatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
    10141 aggcaaactc agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg
    10201 tattactgct aacccgatgc tggggcgttt ctctgactat gtgattgcta acggcggtac
    10261 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
    10321 ccgcgacgaa gcaacgtttg aaaaactggt cactatggtc aacgacttca aacagtactt
    10381 tattgcccat gaccagccga tctatgaaaa cccatcgccg gggaacaaag cgggcggtat
    10441 caccacgctg gaagacaaat cacttggctg tacccagaaa gcgggttcca gcgtcgtggt
    10501 tgacgtgcta cgttacggcg agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc
    10561 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
    10621 cagtactggt cgtggcacgc cgtatggtgg atttgtgccg acggtgaaaa tcgccaccaa
    10681 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca
    10741 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
    10801 cggtaagcaa acctgtaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
    10861 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
    10921 gccgtttgct tttccatacc tgctaaggat ttatcatgac cgcgtattgg ctggcccagg
    10981 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
    11041 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
    11101 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
    11161 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
    11221 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
    11281 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
    11341 gggtgattca caacttctgg gcggggtcga taggcggcac gatgattgag ggtagttttc
    11401 tgcttatgaa tggcctgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc
    11461 cgtttaaagt agagaaaacc cctcccgcca tagacgaaag gggttaaaca attaattacg
    11521 cagggcgcta tttgccagac gatcgtcttc tgcctggcaa gctgccgcag tgaatagcac
    11581 gtcagttgaa gagttcagcg cggtttcgca agagtcctgc aatacgccga tgataaagcc
    11641 gacggcaacc acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagtgg
    11701 gatcagcagc agagaccccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac
    11761 tacgctcaac agcagcgctg tgggcagatc gaccggaata cccagcgtat taaccgcagc
    11821 cagcgtcaac acggtaatgg tgattgctgc gcccgccata ttgatggtgg cacccagcgg
    11881 aatagaaacg gaataggtat cgcgatccag attcagcttt tcacacagcg ccatattcac
    11941 cggaatgtta gctgcagagc tgcgggtgaa gaaggcatac acgccgcttt cgcgcaggca
    12001 cagcagcacc aacgggaacg ggttacgacg aattttccac cacaccagca atgggttaac
    12061 caccagcgcc accagtaaca tacagcccac cagcacgacc agcagttgcg cgtagcccca
    12121 cagtgtggag aaaccggtgg ttgccagggt agaagaaacc agcccaaaaa taccgatcgg
    12181 tgcgaagcga atgaccagtt tcaccataaa ggtgacggca ttcgacatat cgttgaccag
    12241 gtttttggtg gtttcgttac cgtgacgcag tgcgaaaccg aggccaattg cccacaccag
    12301 gatcccgatg tagttacctt tcagcagcgc gtcaatgggg ttggaaacca tgctcattac
    12361 cagtccacgc atcacttcga caatgcctga cggcggcgaa atatcacccg cgctactgga
    12421 caggtgcagg gtagaaggga aggcaaagct gaagactact gcggccagag ctgcagagaa
    12481 ggtgcccagc agatagagga acaaaatagg acggatattg gttttctgcc cgtgctggtg
    12541 gttagcaata gatgccatta ccagcatcaa caccaggatg ggggcaacag ctttcagtgc
    12601 gccgacgaac aaagtaccta acagaccaac agcttccgcc gcgggttttg agatccatgc
    12661 cagaagaatc cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag
    12721 acgccggaat agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttgt
    12781 cgcacatccc tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag
    12841 gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagagc
    12901 tcatgttttg caacttaatg gttacattta tttgacataa acagagggaa ggactggcta
    12961 ctaaggctga ttagataaca tatgggcaaa actatttgga atatgagaac ggttgcgctc
    13021 ctttaatgtg ctatgccata taaagtcact ggcgcaagga gcgcgcaggg gacggccaat
    13081 cgccgccgcc ccctgcaccc ccaggctctg gcgaacaaaa tcgccgctgc gcggttccct
    13141 cagcttatcc cttgcggcta ccggttcggg cgcaagggaa cgcttcggcg attttgacgc
    13201 caccaacacc cgtgcggtct ttttgtaaag agctataagc cgattacata attaatagct
    13261 cacctttgtc tggaatgagt tatctctagt aatggcttaa tatacagcgg atcattctcc
    13321 ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca tcaccaggat
    13381 gccaaacacc acgcccagcg agacggcgat cggaatatgg tagaagtcga caatcagcat
    13441 cttgataccg ataaatacca gaatcaccgc caggccatat ttgagcatcg agaaacgctc
    13501 tgctacgccc gccagcagga aatacatcgc acgcaggccg aggatcgcaa acaggtttga
    13561 ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc tatccacggc
    13621 aaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg tggcgtacag
    13681 caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg
    13741 accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt catgggccag
    13801 cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga tccagtcgaa
    13861 ctgcgaaatc agccagctgc ctgtgaagat catgatggta cgcagaacaa tcgccccgag
    13921 cacgccatac accagcacgc gacgttgtaa tgccgccgga acggagaaat agctgaacaa
    13981 catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat aacccgtgag
    14041 aaaggccagt gcctgtggat cggcaacggc gcgaccttcg gtttgcacca gataccacca
    14101 gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg ccgcagcctg
    14161 tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga tagccagcat
    14221 aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag tattcataga
    14281 agttccttac acataaaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg
    14341 catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat
    14401 tcctttcggg ttgcccggtg accggatgtg atttttcaca catcgtaatg acgatcgacc
    14461 ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc acccagtcaa
    14521 tggtctgtta catgcctttt acactgcttt acggtaacaa ttattgtttt acgctatctg
    14581 ccggaaaaat cacccctgtc tggcggcgga tctctgtcag caatttggcg gtgatgcgac
    14641 tgaccgccag tcccggatga tccaccagat gctcatccac cagggtagcg aacagctctg
    14701 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    14761 gcggcacaaa gcatactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    14821 cgccctgaat ttcgcttgcc aggacagaat cactgacttt tgagtgctgc aacgtaacgc
    14881 tgaaatcacc gtaattcatt accaccacgc cgtgggcatc tacgccgctt gccagcaaac
    14941 tggcggttgc ctgcacgctt ttcggctcac caaataacgc caccgccgac gccaggcagt
    15001 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    15061 caccgtccag gtaacgttga taccgcgagg aatactggca atagttgaaa aaaactttcc
    15121 gcagtttgcc gactttcggc agcgcctggc gcaacaaatg aaagttcggc aggctggcgg
    15181 ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg gcggcatcca
    15241 cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaattttg tggctaagga
    15301 aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc gcgtcaatgg
    15361 catcgctttc cgccatcgct tccagcgacg taaacagatg ctcgacagaa aaatcattgg
    15421 cgaaatgctg ggcctgttca aggctgcggg aataaacggc ggttaacttg tatttaccgc
    15481 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
    15541 gtatcataac gcgtggatac tccataaagg gattctgccg tcaatgtagc acgccttttt
    15601 catcttcgac gtgcgcggat acgcattatt tctgcgctgg aaaaccatgc tcactaaacg
    15661 gaccaataat gaccgacggc ccgagatatt ttggctgacg atggaagatt ttcgcgtcaa
    15721 tcaccgcgct gattctggcg agtttctccc gtaactgagt ataaaaaccg tgcttcatat
    15781 tcaggtctgg tgcgttaaac ccgatagcat cgatgccgta ttgttttgcc agccagatgg
    15841 cgcgttggtt atgaaattct tgcgaaataa tagtgatatg gttttcccca aacacctttt
    15901 tggcacgcac taccgaatca agcgttgaga aaccggcata gtcgcagaag atcactttcg
    15961 ccggtacgcc tttagcgatc aaagcctgct gcatacctga tgcttcatcg taatttttac
    16021 gtccgttatc gccactgact aacagccatt tcacttttcc ggcgtggtac aactctgctg
    16081 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
    16141 cgttacgcgc cggaacgacg ttgacgtcgc tccaggtcag ctgtttgctg gcgttgacca
    16201 ttacgcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct
    16261 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg
    16321 tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac
    16381 gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgggttag ccacagtcgg
    16441 gtgtcgaact caagctggtg gtactggggt tccatatgac agcacaactg gtaaaacgct
    16501 ttgttatgct ctttctcctt caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac
    16561 ggttccggcg cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc tttcagcttg
    16621 ccgccctgca cacgcgaaac agcggtatgt aacccgagcg cgttttttag cacatggatc
    16681 ttattgtcat acatcacttt attgatcggc ggggcattac gcagaaactg atttttcaga
    16741 tcctgagtat attgccagag ggctttatcg gtggcgtagt cgtgtgttcc cggatagcgt
    16801 tttgccagca catcgcccag acgctgttcg ttaatcagtg ttcgcacctg ggaaagtagc
    16861 tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa
    16921 aagggtatac tcacgcaccc ttttcagggg caatgtcgaa attttatcat tcaggagggc
    16981 cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt ccggcgacgg
    17041 atgacgttaa cccgctacag gcgtgggaag cggcggatga atatttgctg caacagttgg
    17101 acgacacaga aatccgcggc ccggtgttga tcctgaatga tgcctttggt gcgttaagct
    17161 gcgcgctggc ggaacataag ccgtacagca ttggcgactc atacatcagt gaactggcga
    17221 cgcgcgagaa tttacgcctc aacgggattg atgagtcgag cgtgaagttt ctcgacagca
    17281 ccgccgacta cccgcaacag ccgggtgttg tgctgatcaa agtgccgaaa acactggcat
    17341 tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac gtcggataca cgtattattg
    17401 ccggtgccaa agcccgtgac attcacactt ccacgctgga actgttcgaa aaagtactcg
    17461 gtacgaccac caccacgctg gcatggaaaa aagcgcgcct gattaattgc actttcaatg
    17521 aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga
    17581 ctatccacaa ccatgcgaat gtcttctccc gcaccgggct tgatatcggc gcgcgcttct
    17641 ttatacagca tctgccagag aatctcgaag gggagattgt cgatctcggt tgcggtaatg
    17701 gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg
    17761 aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt
    17821 tggatcgctg cgaatttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta
    17881 atgctatact ctgcaacccg ccgtttcatc agcaacatgc gctgaccgat aacgtcgcct
    17941 gggagatgtt ccaccacgcc cgccgctgcc tgaaaatcaa cggcgaactg tatatcgttg
    18001 ccaaccgtca cctgaattac ttccataaac tgaagaagat tttcggcaac tgcaccacta
    18061 ttgcgacgaa taataaattt gtggtgctga aagcggtgaa gctcgggcgt cgtcgataag
    18121 gttgagaaca acctgccgga tgcggcgtga acgccttatc cggcctacgt tcagcacgtt
    18181 actcccaggc cggataagac gcgtcaagcg tcgcatccgg caatgtctgc ccagttctgg
    18241 aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc tctacattct cttgtttagc
    18301 gtttttctac gtttattctt ccgtcacaca gataaattca tccgttgcca atctgtcaac
    18361 ggatgttatc atattgccaa tttgtcaacg gacgtgatga agaatgcacc tgataactca
    18421 aaaagcattg aaagatgctg cggaaaaata cccgcaacat aaaacggagt tggtggctct
    18481 agggaacacg attgctaagg gatatttcaa aaaacctgag tcattaaaag cagtattccc
    18541 atctctggat aacttcaaat atctggataa gcattatgtt ttcaatgttg ggggcaatga
    18601 attacgtgtt gtagcaatgg tcttttttga atcgcaaaag tgctacatac gtgaagttat
    18661 gacgcataaa gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt
    18721 gcgattgccg acatcttgca agcaggagaa aagctaactg ctgtggcacc ttttctggcg
    18781 ggtattcaga acgaggaaca atacacccag gcgctggaac tggtagatca tttgctgctc
    18841 aacgatcctg aaaacccctt actggatctg gtgtgtgcca aaataaccgc gtgggaagaa
    18901 tcagcgcccg aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatagcc
    18961 gtgattcgta cccttatgga tcaatatggt ttaacccttt ccgatctgcc ggaaattggc
    19021 agtaaatcta tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct
    19081 aaaaaattgg caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg
    19141 ccggatgcgt cgcatccggc gcttcaaata atgatttaaa tctccagcgc cagccgtgtt
    19201 ccctgggcaa ttgcccgtcg tgcgtccagc tccatagcta catcgcagcc gccaattaaa
    19261 tgcaccgttt tcccgctatc aatcagcggt tgcgccaatg cgcggtttgg ctcttgccct
    19321 gcgcagatca ccacattgtc cactgctaat acctgcggtt cgccgttgat caccacatgc
    19381 agcccgtcat cgtcgatctt ctgataactt acacttggga tcattttcac gccgcgcgag
    19441 agcagggtgg tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg
    19501 gctttgcgct ggagcatcac gatctgccgt gggctacgag ggatctgcat tccctgcggg
    19561 cttaagccac cagcctgttg taggctactg tcgatccccc actcattaca aaactcagcg
    19621 atattctggc tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc
    19681 ccgccacaac cgatgatggc aactttgtta cccaccggcg ctttgtcgcg cagtacatcg
    19741 agataactca ataccttcgg atgatcgatc ccgtcgatgg gcggagtgcg cggcacgatc
    19801 ccactggcga ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg
    19861 agttttagcg tcacgcccgt cacttcgatc atccggtggt aatagcgcag cgtttcgtaa
    19921 aactcctctt tgccggggat ctgtttggcc atattaaact gcccgccgat ctcgctgtgg
    19981 gcatcaaaca atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca
    20041 gcaggtcccg caccgaccac cgccagattt tttttctgca cggcgggaag gattagcatt
    20101 ttggtttcgt ggcaggcgcg aggattcacc aggcacgagg tgaccttgcc aacgaagatc
    20161 tgatcgagac acgcctggtt gcagccaatg caggtgttga tctcatcggc tcgccctgat
    20221 tgcgcttttg acagcagctc cgcatccgca agaaacggtc gcgccatcga taccatatcg
    20281 gcatcgccgc gcgagagaat atcgtcggca acctgcggat cgttaatccg gttggtggtt
    20341 accagcggca gcgagacgtg gcctttcagt ttgcgcgtga cccagctaaa tgcgccgcgc
    20401 ggcacgggcg tggcaatggt cggaatacgt gcttcatgcc agccaatgcc ggtgttgata
    20461 atggtcgcgc ccgccgcttc aatggcctgc gccagctcta ccgtttcggc aaaagtcccg
    20521 ccgtcttcga ccaggtcgag catcgacagt cggtagataa taatgaagtc gttgccaacg
    20581 cgttcgcgta ccgcactcac cacttcaacg gcaaaccgca tccggttgtg gtaatcgccg
    20641 ccccactggt cactgcgctg attggtgcgc agcgtcagaa attcgttgat caaataccct
    20701 tcggaaccca tcacctctac accgtcgtat cctgcctccc gtgccagttg cgcgcagtgg
    20761 gcgaaatcgt cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg
    20821 ttgatggggg cctgcaatgc ggagggagcg accagatgcg gttggtagct gtagcgcccg
    20881 gtatgcaaaa tttgcagggc tattttgccg ccttcctgat gtaccgcttc ggtaatggtg
    20941 cgatggtgtg ggatctggct ggcgtcgttg agcattgcgc caccttccat gccaacgcct
    21001 gttaaatctg gtgcaatgcc gccgctaaca atcagcgcca cgccgtgacg ggcgcgttcg
    21061 gcataaaacg cagccagccg ttcagcgccg tccgggtatt cctccagccc ggtgtgcatt
    21121 gaacccatca acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc
    21181 gacgggtagc tcataaaatt gtccagtatg taaaataatt gttatgtggt cggatgagtt
    21241 ctaatttagc cgtcgccgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc
    21301 aaaaagaata cgcccattgg aaatgccgga tgcgacgctt gtcgcgtctt atctgtatat
    21361 ggacacctct cgttgtgcaa gtactgtgtt cgaaaaatca ataggtaaca ggaggtctga
    21421 ctgacaggac ggcaggctat ggagagagta aatcacgctc tctttagccc gctcatccgg
    21481 ggtcatcttc gaccatttca ggatgacttc gtcggtaatg tgggcctttt ggtccctttc
    21541 tgtcttgccc ggacggaggg caattgcggg ccagactcat gttccgcact ctgatttcca
    21601 tccatcagta agggctgtga agacctgaaa cttcggatgt ttttcccggg agggctggct
    21661 cttttggcca ctttc
//
LOCUS       DAZUGK010000067        21456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZUGK010000067 DAZUGK010000000
VERSION     DAZUGK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.067"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..1539
                     /gene="caiT"
                     /locus_tag="ACHY5W_002179"
     CDS             25..1539
                     /gene="caiT"
                     /locus_tag="ACHY5W_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFS4447073.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSFFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1570..2712
                     /gene="caiA"
                     /locus_tag="ACHY5W_002180"
     CDS             1570..2712
                     /gene="caiA"
                     /locus_tag="ACHY5W_002180"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFS4447074.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMNKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2841..4058
                     /gene="caiB"
                     /locus_tag="ACHY5W_002181"
     CDS             2841..4058
                     /gene="caiB"
                     /locus_tag="ACHY5W_002181"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFS4447075.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4132..5685
                     /gene="caiC"
                     /locus_tag="ACHY5W_002182"
     CDS             4132..5685
                     /gene="caiC"
                     /locus_tag="ACHY5W_002182"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFS4447076.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5794..6579
                     /gene="caiD"
                     /locus_tag="ACHY5W_002183"
     CDS             5794..6579
                     /gene="caiD"
                     /locus_tag="ACHY5W_002183"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFS4447077.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6585..7175
                     /gene="caiE"
                     /locus_tag="ACHY5W_002184"
     CDS             6585..7175
                     /gene="caiE"
                     /locus_tag="ACHY5W_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFS4447078.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDKLHWKLLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7261..7656)
                     /gene="caiF"
                     /locus_tag="ACHY5W_002185"
     CDS             complement(7261..7656)
                     /gene="caiF"
                     /locus_tag="ACHY5W_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFS4447079.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7917..11138)
                     /gene="carB"
                     /locus_tag="ACHY5W_002186"
     CDS             complement(7917..11138)
                     /gene="carB"
                     /locus_tag="ACHY5W_002186"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4447080.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11156..12304)
                     /gene="carA"
                     /locus_tag="ACHY5W_002187"
     CDS             complement(11156..12304)
                     /gene="carA"
                     /locus_tag="ACHY5W_002187"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFS4447081.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12761..13582)
                     /gene="dapB"
                     /locus_tag="ACHY5W_002188"
     CDS             complement(12761..13582)
                     /gene="dapB"
                     /locus_tag="ACHY5W_002188"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4447082.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
     gene            complement(13749..14663)
                     /gene="rihC"
                     /locus_tag="ACHY5W_002189"
     CDS             complement(13749..14663)
                     /gene="rihC"
                     /locus_tag="ACHY5W_002189"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFS4447083.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALAP"
     gene            complement(14729..15679)
                     /gene="ispH"
                     /locus_tag="ACHY5W_002190"
                     /gene_synonym="lytB"
     CDS             complement(14729..15679)
                     /gene="ispH"
                     /locus_tag="ACHY5W_002190"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFS4447084.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15681..16130)
                     /gene="fkpB"
                     /locus_tag="ACHY5W_002191"
     CDS             complement(15681..16130)
                     /gene="fkpB"
                     /locus_tag="ACHY5W_002191"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4447085.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16218..16712)
                     /gene="lspA"
                     /locus_tag="ACHY5W_002192"
     CDS             complement(16218..16712)
                     /gene="lspA"
                     /locus_tag="ACHY5W_002192"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4447086.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16712..19528)
                     /gene="ileS"
                     /locus_tag="ACHY5W_002193"
     CDS             complement(16712..19528)
                     /gene="ileS"
                     /locus_tag="ACHY5W_002193"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4447087.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19571..20512)
                     /gene="ribF"
                     /locus_tag="ACHY5W_002194"
     CDS             complement(19571..20512)
                     /gene="ribF"
                     /locus_tag="ACHY5W_002194"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFS4447088.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20520..20738)
                     /gene="yaaY"
                     /locus_tag="ACHY5W_002195"
     CDS             complement(20520..20738)
                     /gene="yaaY"
                     /locus_tag="ACHY5W_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFS4447089.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20841..21104
                     /gene="rpsT"
                     /locus_tag="ACHY5W_002196"
     CDS             20841..21104
                     /gene="rpsT"
                     /locus_tag="ACHY5W_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4447090.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5079 a         5760 c         5531 g         5086 t
ORIGIN      
        1 tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg aatagaaccg
       61 aaggttttct ttccgccgtt aataatcgtc ggcatacttt gttggcttac agtcagagat
      121 ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa tgtatgggga
      181 tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct ggtgtttggc
      241 ccgtatgcca aaaagcgttt aggtaacgaa ccgccagagt ttagcaccgc cagttggatc
      301 tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc gattgagatc
      361 tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg agcgaaagag
      421 ttggggctgg cttacagctt tttccactgg ggacctctgc cgtgggccac ttacagcttc
      481 ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc
      541 tcgacactgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg taccatcgtc
      601 gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg ccttgccacg
      661 ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcatacctt gcaactggac
      721 gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcctg cggtctgcaa
      781 aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat gctgggttgg
      841 gtgttcattg tcagcggtgc cagcttcatc atgaactact tcaccgattc ggtggggatg
      901 ttgctgatgt atctgccgcg aatgttgttc tataccgatc ccatcgctaa aggcggcttc
      961 ccgcagggct ggaccgtgtt ctattgggca tggtgggtga tttacgccat ccagatgagt
     1021 atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt cggcatggtg
     1081 ctgggactga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa cactctgctg
     1141 ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg tgtggcgcgc
     1201 gccattatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc
     1261 atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac cctggcgatg
     1321 tccacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tgctggtgcg tatcggttgg
     1381 tcaattctgg ttggcattat cggtattgtt ttgctggcgc tcggcggcct gaaaccgatt
     1441 caaactgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat ggtgacgctc
     1501 tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa aatatcaaga
     1561 ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc
     1621 gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc
     1681 tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct gctgatccct
     1741 gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg gatggagctg
     1801 ggacgtctgg gggcaccaac ctatgtactg taccagctgc cgggcgggtt caataccttc
     1861 ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg caccggtaag
     1921 cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg tagcctgaaa
     1981 acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg ttttattacc
     2041 agtagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc tccggacaaa
     2101 cctgtctaca ccgaatggtt tgttgatatg aacaaaccgg gcatcaaagt gaccaaactt
     2161 gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga tgtggaactg
     2221 gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa agaagagttc
     2281 gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg cgcctttgaa
     2341 gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg tcgtttccag
     2401 ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa aaacatgctg
     2461 tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc agcgatgtgc
     2521 aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca ggtgctgggc
     2581 ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct gcgtgtagac
     2641 cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgtgc ggtgctgaag
     2701 caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat caggcctacg
     2761 ggagcacgaa tgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgctacat
     2821 aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcggg ccgttggccg
     2881 gattgcgcgt tgtcttctcc ggtatcgaaa ttgccgggcc gttcgccggg caaatgttcg
     2941 cagaatgggg tgcggaagtt atctggatcg agaacgtcgc ctgggccgac accattcgcg
     3001 ttcaaccgaa ctacccgcaa ctctcccgcc gcaatttgca cgcgctgtcg ttaaatattt
     3061 tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat atcttcatcg
     3121 aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta ctgtggcagc
     3181 acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc accgaggagt
     3241 acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac ctgattcaga
     3301 acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac ttttctggcc
     3361 tgaccgccac cacggcggcg ctggcagcac tgcataaagc gcgtgaaacc ggtaaaggcg
     3421 aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac ttcatgatgg
     3481 attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat ccctactacg
     3541 ccggttgcgg tctgtataaa tgcgccgacg gctacatcgt gatggaactg gtgggcatta
     3601 cccaaattga agagtgcttt aaagatattg gcctcgcaca tctactcggt acaccggaaa
     3661 tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca ctggtagaag
     3721 aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa cgctttgccg
     3781 aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc aatccacagt
     3841 atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc tgcaaagggc
     3901 cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga atgccctcac
     3961 atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac gacattcagg
     4021 agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc agtcggcttg
     4081 cctgactaac gcccgcccaa taacaaacga acaaaaatgg atagaggtgc aatggatatc
     4141 attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta cggtcataaa
     4201 acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta tcttgagtta
     4261 aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg caaaggcgac
     4321 aaggttgcac tacatctcga caactgcccg gaatttatct tttgctggtt cgggctggca
     4381 aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga aagcgcgtgg
     4441 atcctgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaatttta tcctatgtat
     4501 caacagattc agcaggaaga tgccactcaa ttgcggcaca tttgcctgac agatgtggca
     4561 cttcccgctg atgatggcgt gagttcgttt actcaactga aaaatcaaca acctgccacc
     4621 ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt cacctccggc
     4681 accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt cgctggatat
     4741 tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acctgacggt aatgcctgcg
     4801 tttcatatcg attgccagtg tactgcagcg atggcggcgt tttctgccgg tgccaccttt
     4861 gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggtgcagaa gtaccgcgcc
     4921 accattaccg aatgtattcc gatgatgatt cgtacgttga tggtgcagcc gccttcagcg
     4981 aacgatcggc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc ggagcaggaa
     5041 aaagatgcgt tttgtgaacg cttcggcgtt cgcttgctga cgtcttatgg gatgacggaa
     5101 accattgtgg gcattatcgg cgatcgccct ggcgataaac gacgctggcc gtcgattggt
     5161 cgggcggggt tttgctacga agcggagatc cgcgacgatc acaatcgccc gctcccggca
     5221 ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt caaagagtat
     5281 tttctcaacc cgaaagccac tgcgaaagtg ctggaagccg acggctggct acataccggc
     5341 gatacaggat actgcgacga agaaggcttt ttttatttcg tcgatcgccg ctgcaatatg
     5401 atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatatcat cgccacccat
     5461 ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgattcgcga tgaagccatc
     5521 aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt ttttcgcttc
     5581 tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag aaaagatctg
     5641 ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa acggcaggga
     5701 actaccctgc cagctaataa tcacgctcta aaacaatatg cattttttgc agggatgttg
     5761 tcgtccctga aaaagcaaaa atggagaaaa gcaatgagtg aatcattaca tctgacccgc
     5821 aatggatcaa ttctggaaat tacccttgat cgcccaaaag cgaatgctat tgatgcaaaa
     5881 accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca attacgtgtc
     5941 gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt aaaagcggca
     6001 gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg attaaccgaa
     6061 attttcaatc tcgacaaacc ggttatcgca gctgtgaacg gctacgcctt tggcggcggt
     6121 ttcgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag cttcgccctg
     6181 ccggaagcca aactgggcat cgttcctgac agcggcggtg tgctgcgtct gccgaagatc
     6241 ctgccgcctg ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg cgcagaagag
     6301 gcgctgcgtt gggggatagt caaccgcgtg gttagccagg cggaactgat ggataacgcc
     6361 cgcgaactgg ctcagcagct ggttaacagc gccccgctgg cgattgcggc gctgaaagag
     6421 atctaccgca ccaccagcga aatgccggta gaagaagcgt atcgctatat tcgcagcggc
     6481 gtgttgaaac actatccgtc ggttctgcat tcggaggatg ccattgaagg gccgctggcg
     6541 tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc tattacgcct
     6601 ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt gccgtcttga
     6661 ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca ctgcgtggtg
     6721 actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc attatgcatg
     6781 gctactgcga cactgacacc atcgttgggg aaaacggcca tatcgggcac ggagcgatcc
     6841 tgcatggttg tgtgattggt cgcgatgcat tggtcgggat gaacagcgtg attatggatg
     6901 gcgcggtcat tggcgaagag agcattgttg ccgcaatgag ctttgtcaaa gcgggctttc
     6961 gcggcgagaa acgtcagttg ttgatgggta cgccagcccg cgcggtacgc actgttagtg
     7021 acgacaagtt acactggaaa ctgttgaata ccaaagagta tcaggatctt gtcgggcgct
     7081 gtcatgcatc gttacatgaa acacagccac tgaggcaaat ggaggaaaat cgcccccgtt
     7141 tgcaggggac gacggatgtg acgcctaaac ggtgatcttg ccggatgcga cctggttgct
     7201 ggataagatg ctacaacatc gtatctggca agcaggacgc ccggtatcaa cgagataaaa
     7261 ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt taagttccgt
     7321 gagcggaacg gcaggaatac gcggtgtctt tcttacgccg actaattttt cccgactgtt
     7381 attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc actttaacca gtcgctgaca
     7441 ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc ttatttctgt
     7501 gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg
     7561 aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt cggcgattaa
     7621 caagtagagt ggtttttcaa catatccttc acacatacga actccagttt cacaaattaa
     7681 aagtctggct atgactcgtt ttgaagcaat taaacaaggc catattaaaa ttgtggatat
     7741 atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa
     7801 aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaacggtcct catcagagaa
     7861 ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccatgac acgctattat
     7921 ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc ggcattcagc
     7981 gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt atattgcagc
     8041 gcactgcgac ggatcacgcg ggagtcttca atcgcacgac gacctgaggt ggtgttgatg
     8101 atgtaggtat attcgccatt cttaatacgg tcctgaatgt gcggacggcc ttcatgcacc
     8161 ttgttcacca gacgcggatt gatacctgct tcgcccagca caatcgccgt gccgtgggtc
     8221 gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta
     8281 tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc
     8341 agctgcgctt tggcaaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta
     8401 gagcgcattt ctggccctaa cagcgggtca acgcccggga atttattgaa cggcagcacc
     8461 acttctttca ccgagtagta cggcgggata acttctttgg ttacgccctg ctcagccagc
     8521 gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag
     8581 acgaacggaa cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac ttcgttgttt
     8641 ttcaccgcaa actgcacgtt catcaggcca cgcacctgca attcgaaggc cagtttctgc
     8701 acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtaggctgg cagagaacat
     8761 gcggagtcac cggagtgcac gcccgcctgc tcaatatgct ccatgatgcc gccaatcagc
     8821 accatttcgc cgtcgcagat ggcatccacg tcaacttcta ccgcgtcatc gaggaagtgg
     8881 tccagcaaca ctggcgcatc gttagacacg ctgaccgccg tctggaagta gcgacgcagg
     8941 tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgtacc
     9001 accagcgggt agccaatctc tttcgccttc tctaccgcca tttcaatagc ggtaacggtg
     9061 gcgttcgccg gttgtttcag tttcagacgc tcaaccgcat gctggaagcg ttcacggtct
     9121 tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc ggcttccagt
     9181 gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc tttcggcttc
     9241 tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta gaggcgatcg
     9301 gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat ggtttcgtaa
     9361 ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc gataccctga
     9421 ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt agacggattc
     9481 gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc aaactctgcc
     9541 gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg cagcttacgg
     9601 atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt
     9661 ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc cactttctct
     9721 tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg
     9781 aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct
     9841 gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc atccaggctc
     9901 actttcgggt cgaatccagt cgcaccgact tccaggccgc gcagcgcttt ttgcagggat
     9961 tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga
    10021 cggtcgttag cgccggcgaa tttttcgaag ttgaagcgag gaattttagt aaccacatag
    10081 tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt catcagttca
    10141 tcgagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa accggtcgct
    10201 ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac aatcaggcga
    10261 ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac gccgatttca
    10321 cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcggt cagcgtttgg
    10381 gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata
    10441 gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc
    10501 cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc cagaccgcgg
    10561 gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct accgcccatg
    10621 gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaaccgc cagcgcttct
    10681 tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt catcgctacg
    10741 tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc aatcatggtg
    10801 acgccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc
    10861 tgaccgccca tcgttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc
    10921 acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg
    10981 gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag ggctttacat
    11041 gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc
    11101 agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact
    11161 tagcggtttt acggtactgc tcaattaact cgataaagtg gtcgaacaac ggcgcggcgt
    11221 cgtgtggacc agggctggct tccgggtgcc cctggaaact gaacgccggt ttatcggtgc
    11281 gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag
    11341 gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt
    11401 tctcaacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct
    11461 tcgcaccgct cgccagcgcc agcagctgat gaccgaggca gatgccgaat accggaatat
    11521 cggtttcgag gaatttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag
    11581 gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt
    11641 gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg atgttgcgct
    11701 tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt ttcgcttctg
    11761 gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct gcggtggtca
    11821 cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaacgcca
    11881 gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca
    11941 gtaaacgtgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgcttca
    12001 ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc agatcgcgaa
    12061 tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta gtgccgacat
    12121 tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga
    12181 tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg
    12241 cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa
    12301 tcaaaacacc ctccagagaa tattcactca ctttatttgc atattaattc acaatgatga
    12361 cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga
    12421 tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt ggctcatgca
    12481 tgttttacag gcattaagcg acaaaaagat catctaacct gttctggaaa acgcttgcgc
    12541 agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat ggtcaaaatt
    12601 tacattaaaa caacaaaaat agaagacaaa acatgtaatt tttggcagtt atagaaaatt
    12661 taaaagcata acaccaaaaa ataaaagggc taaaaattaa agccctttat aattaataaa
    12721 ttaaatgtgt tacttttgca tcatcataaa cattttgtgg ttacaaatta tggagatcaa
    12781 gcacatctcg catatcaaaa agaccacttt ccttaccact caaccacaaa gccgatctta
    12841 ccgcaccgtt agcaaatgtc atgcggctgg acgccttatg ggtgatctcc agacgctcgc
    12901 caatatcggc aaacatcgcg gtatgttcgc caacgatgtc acctgcacgc acggtggcaa
    12961 aaccaatggt accaggcaca cgttcaccgg tatggccttc acgactgtag accgcgcaat
    13021 ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt gcggtgcctg
    13081 acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg gtgtagtcac
    13141 ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca acgctaaaat
    13201 tggcggcaaa gacaatcgca atatcggcag cggcgtcacg aattgcctgt ttaccggctt
    13261 cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctggcgacaa aaagcgagat
    13321 ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca tcttttatcg
    13381 catcaagact gctttgcacg gtaacgcctg ttttccctgc accagccagc tcaccggcgt
    13441 cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc acgccttcta
    13501 atgccagcgc cgcctgaatc aactggcggc ccatacgccc cccggctccc gcgatggcaa
    13561 cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag accgttttca
    13621 gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga aattaatgat
    13681 aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg ccaataacat
    13741 gtgacaggtt acggcgccag agccagcacc tcagccaccc actgctggaa gccttttaca
    13801 ttcagatcca atgccacctg tacattggct ggtttgccca ggcaaccgtc gatatcaacc
    13861 accgtcgtgc ctgaggtaaa ttcgccttga gtttctaccg cgacaaaaca cggcttgagg
    13921 gtgaataagt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat tcgcagaccg
    13981 ctttgcatac tgccgctacg gtagtggcta aacagggcgt ggagcatttt tccggtgcga
    14041 ttcaactccg gtaaagtagc gagatagtca ggggttaata ttgcctgatt ggtgacatcc
    14101 agaccgcaca tgacgatttc gatgccgcta cggaagacac aggctgccgc ttctggatcg
    14161 gcagcgatat taaactcggc gtttggtgta cagttgccgc gaccagcaga accgcccatg
    14221 atcaccagac gacgaatata cggtttacat tccgggcatt gtgaaagtaa cagcgcaata
    14281 ttggttaacg ggccaatggc caccagggta acaggctctg gtgcacgcat cagggcatcc
    14341 cgaatcgcca gaaacgccgg tatcccgagt ggctttcggt tgtgctcaac aaagtcgtag
    14401 ccagccattc ccgattcgcc gtgcacagat gctgcatcac gcggtgcgcg taccagcggc
    14461 acagcggctc cctgggcgag cggaatctcc gcattccaga aatgcagcag ttgcaaggcg
    14521 ttacgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag ttgcaggtcg
    14581 agttcggggg caaaaatcgc ggcagcaatg gcgacggcat cgtcaatgcc ggggtcagta
    14641 tcgaggaaga taggtaaacg catgttttct ccataaaaaa tgccggtaac aagaccggca
    14701 ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct
    14761 ctttcggcac ttcgaaaaca atgttttctt cacgtccttc cagcggaatg gcttcaccac
    14821 cgcccagctg ttgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc
    14881 ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat
    14941 cgtcaatcaa aaacgcgcgt ttgcccatgc gctgggccag ttccgccaga cggttggagt
    15001 tggaggagtt tttcgaaccg accaccagca ccacttccgc ctgctctgcc agggcgcgta
    15061 ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt
    15121 tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg
    15181 tttgggtcat aaaggagagt ttctcttcgt ttttgaccgt cagtttccac acatcgtctg
    15241 gcgattcgac cagatacatt cccccttccg ggttgctgta ctgccccatc gtcccttcca
    15301 cttccgggtg cccggcgtgg ccgatgagaa tcgattcttc gccacggcgg ctggcgcggg
    15361 cgacttccat atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc
    15421 ggctttttgc ttcgttacgt accgcctgag aaacaccgtg tgcggagaaa atcaggatcg
    15481 cgccgtccgg tacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaggctat
    15541 cgaccacgta gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg
    15601 ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt
    15661 tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata
    15721 tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg
    15781 ttaatttcgc ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt
    15841 gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg
    15901 tccggtgacg gcacgccaaa cgccgcatcg ggctccagtg agaaggtggt tttatcgccc
    15961 actttcagcc ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg
    16021 aacagcgccg gtttaccgtt gttgcgggta gactcggcgg tggtgccatc gtcgagtttt
    16081 agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc
    16141 tcttatattt tgaggatttt tgccggatgc ggcctacgat ttcgcagcaa tcggtaggcc
    16201 ttatccggca gggtttatta ttgttttttc gctttagaag gcaaaaaacc ttccagcaca
    16261 atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc gaagtgccag
    16321 tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg gtcgaacagg
    16381 ttgcccagcg cgccgccaat aatcagcgca taagcgatat tgtttagctt ctgcgtggcc
    16441 ttcgagcgat acatcatcac tgccaggatc acgctaatac caatcgcaat accggcaaag
    16501 aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc atagttacgc
    16561 gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc aaagttctgg
    16621 aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag ccacagccag
    16681 cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt tcaccgtcac
    16741 cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc ttgccgacat
    16801 cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc aacgcgactt
    16861 tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta tagtctgcaa
    16921 cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg gtcagtttcg
    16981 ccgccagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca cccactttct
    17041 tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc aacagctcgt
    17101 cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacaggcct tcgtaccact
    17161 caccggtgaa gacgtatttt tcacgttcgc ccggcaggta gccccacact tcatcagcgg
    17221 tgaaggagag gattggtgcc atccagcgca ccagcgcttc agcgatgtga tacagcgcag
    17281 tctggcagct acgacgcgcc acgctatccg ctttggcggt gtactgacgg tctttgatga
    17341 tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc tgtaccactt
    17401 cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct ttcgcacaac
    17461 ctacggccca gcgatcaagt accaccatct cttccggttt caccatatct tttgctggat
    17521 caaaaccatt caggtttgcc agcaggaagc gcgcggtgtt acggatacga cgatagctat
    17581 cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag tcggttgatg
    17641 ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc ggcgaaacgg
    17701 tgttgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag ccgtgggtca
    17761 gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg gaagacatga
    17821 accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga ccggcaaatt
    17881 ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat acatccaatg
    17941 tgtccggcac tttcacgtac tgatcagctt catcgccgag gatctctttc gcatcaagat
    18001 cccaccacgc ctggatgccg tcaacttcaa cgcgttttgc cacttcttcc attagttcga
    18061 gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc ggtacgcccc
    18121 aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac tcgatacgcg
    18181 cctggcccca gtccgggatc cactgcacgc ctttgatctc tttcagtgac tgcgcacgca
    18241 gacctttctg atccatgctg acgaaccact gcggtgtcgc gcggaagatg atcggcgttt
    18301 tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc agcgcgcctt
    18361 tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg ccatccagcg
    18421 tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg gtttccaggc
    18481 cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg tgaacggcac
    18541 cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg aaacccataa
    18601 acggatgggt aaagcgcagc agctcaagct ccgcaccttt taccgtgccg agaatggtgt
    18661 aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc agaatcacgg
    18721 cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag attgcgcggt
    18781 tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg ccgttaacgt
    18841 tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa gcgacgtcaa
    18901 tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca gaacggcagt
    18961 ctacgcacca gtgaactggc ttcgcgcctt tgtgcaggtg accgttgccg atgattttgc
    19021 ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat ggtcaggtac gggtgcgacc
    19081 agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc tgggtcgccg
    19141 cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc ggtttaccgt
    19201 attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca ggcacatacg
    19261 gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga atcttgttaa
    19321 ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc agaatgaagg
    19381 tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca gtccaacgcg
    19441 ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct gtttccggca
    19501 aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc ggtttgatta
    19561 cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt aattcatcac
    19621 gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg cgtatttttt
    19681 tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct aacaaatgca
    19741 cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt gccacgccgg
    19801 gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc accggggaaa
    19861 cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct aattcatcac
    19921 cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct gccagagcca
    19981 gattgtcatc cgccagcgcc tgacgcacgg cggtgctgct gatacgcact ccaccttcgc
    20041 aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc tgtaataaca
    20101 agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc acctacggca agaaatttta
    20161 cgcgcaaatg cttcaccaga agatcgctga tgaaattttg cgcggttaac gccgcgaaac
    20221 gcctgtcgaa acgcacgcac agcacgtaat caacgccaca ctctgcaagg taacgcagtt
    20281 tttcccgcag ccgggtcagc cgtgccgggg ctttatcggt agcaaacagt tccagtggtt
    20341 gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct tcctgcaagc
    20401 cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata gtcagcacac
    20461 acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc atgtctggct
    20521 caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa ccggcgattg
    20581 agtaccgaaa tcagaaagaa gtggcttttc attgccatgg cgcaaatcac gggaagaaac
    20641 tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt cacaccccgc aagctggtag
    20701 aatcctgcgc catcactacg taacgagtgc cggcacatta acggcgctta tttgcacaaa
    20761 tccattgaca aaagaaggct aaaagggcat attcctcggc ctttgaattg tccatataga
    20821 acacatttgg gagttggacc ttggctaata tcaaatcagc taagaagcgc gccattcagt
    20881 ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat gatgcgtact ttcatcaaga
    20941 aagtatacgc agctatcgaa gctggcgaca aagctgctgc acagaaagca tttaacgaaa
    21001 tgcaaccgat cgtggaccgt caggctgcta aaggtctgat ccacaaaaac aaagctgcac
    21061 gtcataaggc taacctgact gcacagatca acaaactggc ttaatcgcca atttgctgaa
    21121 gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc tgctgctttt gtgatgcgtg
    21181 agagaacaca ctgccggttg ccagatagtc aaaagttgca ggttcttgca gacatgccgt
    21241 tgtactatct gaactcttcg tggcttaaga atcgtagaac catcggctga tacattctga
    21301 acaagaatat ctactcaatc tccagccccc ctacaaagaa tgtggatatt gtgatacaca
    21361 ttgaggcatt aaacttaaga ccactctatt tttgaattaa attcctatat actttaacca
    21421 ttcataaata tctcctcatt agaccttgta aattat
//
LOCUS       DAZUGK010000068        21040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZUGK010000068 DAZUGK010000000
VERSION     DAZUGK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.068"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(102..983)
                     /gene="prmA"
                     /locus_tag="ACHY5W_002197"
     CDS             complement(102..983)
                     /gene="prmA"
                     /locus_tag="ACHY5W_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4447091.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(995..2446)
                     /gene="panF"
                     /locus_tag="ACHY5W_002198"
     CDS             complement(995..2446)
                     /gene="panF"
                     /locus_tag="ACHY5W_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFS4447092.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2436..2678)
                     /locus_tag="ACHY5W_002199"
     CDS             complement(2436..2678)
                     /locus_tag="ACHY5W_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFS4447093.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2787..4136)
                     /gene="accC"
                     /locus_tag="ACHY5W_002200"
     CDS             complement(2787..4136)
                     /gene="accC"
                     /locus_tag="ACHY5W_002200"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4447094.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4147..4617)
                     /gene="accB"
                     /locus_tag="ACHY5W_002201"
     CDS             complement(4147..4617)
                     /gene="accB"
                     /locus_tag="ACHY5W_002201"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4447095.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5483..6313)
                     /locus_tag="ACHY5W_002202"
     CDS             complement(5483..6313)
                     /locus_tag="ACHY5W_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447096.1"
                     /translation="MKSLFNRLTGKAVSRTAFVEHLGQEVVQHNPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6350..7324)
                     /locus_tag="ACHY5W_002203"
     CDS             complement(6350..7324)
                     /locus_tag="ACHY5W_002203"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFS4447097.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            7476..9416
                     /gene="csrD"
                     /locus_tag="ACHY5W_002204"
     CDS             7476..9416
                     /gene="csrD"
                     /locus_tag="ACHY5W_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFS4447098.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRMVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9721..10764
                     /gene="mreB"
                     /locus_tag="ACHY5W_002205"
     CDS             9721..10764
                     /gene="mreB"
                     /locus_tag="ACHY5W_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFS4447099.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10830..11933
                     /gene="mreC"
                     /locus_tag="ACHY5W_002206"
     CDS             10830..11933
                     /gene="mreC"
                     /locus_tag="ACHY5W_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4447100.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPPRATPPQSGAQPPARAPGGQ"
     gene            11933..12421
                     /gene="mreD"
                     /locus_tag="ACHY5W_002207"
     CDS             11933..12421
                     /gene="mreD"
                     /locus_tag="ACHY5W_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4447101.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            12430..13023
                     /gene="yhdE"
                     /locus_tag="ACHY5W_002208"
     CDS             12430..13023
                     /gene="yhdE"
                     /locus_tag="ACHY5W_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFS4447102.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSSQTHQ
                     VMTAVALADSQHILDCLVITDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            13013..14482
                     /gene="rng"
                     /locus_tag="ACHY5W_002209"
     CDS             13013..14482
                     /gene="rng"
                     /locus_tag="ACHY5W_002209"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFS4447103.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14550..18350
                     /gene="yhdP"
                     /locus_tag="ACHY5W_002210"
     CDS             14550..18350
                     /gene="yhdP"
                     /locus_tag="ACHY5W_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFS4447104.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSTEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGAPLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18506..19951
                     /gene="tldD"
                     /locus_tag="ACHY5W_002211"
     CDS             18506..19951
                     /gene="tldD"
                     /locus_tag="ACHY5W_002211"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFS4447105.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(20079..21008)
                     /gene="aaeR"
                     /locus_tag="ACHY5W_002212"
     CDS             complement(20079..21008)
                     /gene="aaeR"
                     /locus_tag="ACHY5W_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFS4447106.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
BASE COUNT         4992 a         5341 c         5713 g         4994 t
ORIGIN      
        1 aaattttggc acgcaaattt gcgtaaataa tcggcaaatt tacgtgcctt ttgtgagctt
       61 gctcgcactt cgccccgcgt caccctacgg cgatgcgaag gttaattctt acgaccggta
      121 atacggcacc actcttcttt ttccacgacc gggtccagtg cgaagctgtc ggcataagct
      181 tcacaaacgc tctctgcctg gcttgccaga ataccggaaa ggcccagcaa accgcctgaa
      241 accggcagga cgctgattaa cggtgccagt tcacgtaatg ggcctgcaag gatgttagcg
      301 accaccacgt cggctttcat ttcttctggc tgatctttcg gtaagtagag ttccagacgg
      361 tcagaaacgc cattacgttc ggcgttatcg cggctggcct gaatcgcctg cggatcgata
      421 tcaataccaa tggcttttgc tgcacccagt ttcagcgccg cgatcgccag aatgccggaa
      481 ccacagccaa agtcgatgac tgttttaccg gttaaatcga ggctgtcgag ccattgcagg
      541 cacagagagg tggttggatg ggtacccgta ccaaacgcca gccctggatc taacatcacg
      601 ttgacggcgt tttcgtccgg cacatcacgc cagctagggc agatccacag tcgttcacca
      661 aagcgcatcg ggtggaaatt atccatccat tcgcgctccc agtctttatc ttctagttgt
      721 tcgattttat gcgcgaagcc tgcgccgagc agcggatggt tttccagaat cgccaccacg
      781 tcgttcatat cggtttcagc gtcgaacaga ccaatcacat cggtgtcgcc ccacaggcgc
      841 gtttcgcccg gcagcggctc aaatactggt gtatcgtggg tatcctgaaa agtgatagaa
      901 acggcacccg cttccatcag cgcatcgcta agatcttccg cgttcgcgcc ggtggtgttc
      961 agtttcagtt ggatccaagg catggcaaaa ctctttattt atcagtagtc aaaacggtag
     1021 cttgcgggac ggatgtaccg aaacggtttc cgaccaggaa agccagcaaa cttagtagta
     1081 acgagggcac gatagggtgg aagcccaggt actgaatatt cagcgtcgcg agtacggcat
     1141 acagcacgcc gccaacgatc atcgcactta gcgcgccttt ggcgttggcg cgttcccagt
     1201 aaagacccag caccagcggc cacaggaaaa cggcttccag cccaccaaag gccagcaaat
     1261 tcagccagat gatcatttct ggcggcttcc aggcggcaag cagcagcaat gcgccgagaa
     1321 ctaacgtaat taccgccgac atccgtttca gacgcgtctc gttttgcatt tgatccggac
     1381 ggatattcag atagagatct ttaatgatcg tagcggaact ttgtagcaat tgggcgttaa
     1441 ttgtcgacat gatcgcagcc ataggtgcag ccagaaagat cccggcagca aacggtggca
     1501 gcacttttac cattaacgtc gggatcacca gatccggtac ggtgagatcg gggatcactg
     1561 cacgacctaa cgctccggcc aggtgcatac cgaacatcag gattgcgact acaatcgtac
     1621 cgatgatgat cccccggtgt actgctttgc tgtctttata agagatacag cgcaccgcag
     1681 tatgcggcag gccaatcacg ccaaaacaca ccagcaccca gaacgacgtc ataaaggcag
     1741 gcgacagaat atcgtcagcg ccttgtggcg taaccagttg cggatcgatg gtttgcaacg
     1801 tctgtactgc gttgcttaag ccgccagcgg catgtactac gccaataagc agcacaatgg
     1861 tgccaatcag catcaccaat ccttgcatgg tgtcgttcag cacgctggcg cgaaagccgc
     1921 caaaggcggt gtagagcgca atgctgatac caaaaatcag caaacctgtt tcataaggaa
     1981 tacccgccgc ggtttccagc agacgcgcac cgccgataaa ctgcacggtc atcgcaccaa
     2041 cgaacgcaac cagcaaactc aaactcgcca gccacaccag aagacgactc tggtagcggg
     2101 caaacagcat gtcgttcagt gtcaccgcat tgtagcggcg cgcaagaatc gcgaacttct
     2161 tgccgagaat accgagcgaa agccagactg caggaagctg aatcatcgcc agcaataccc
     2221 agcccaggcc gtatttataa gcagctccag gcccgccgat aaacgaactg gcactgatat
     2281 aggtcgcggt gagcgtcatc gccagcacaa taccgcccat agagcggctg ccgaggaaat
     2341 actcattaag gaaggtgccg gtgctccgtt tacgcatcgc ataaacagag ataccgaaca
     2401 ccaccaccag ataggcgacc agcggtagaa tcacttcaag ctgcatcgtc atcctccagt
     2461 gggatatcgc gatagataaa tttcaccatc gcccagcaca gtccaataaa cagcagcggc
     2521 gtcaggatgc aggccatctc aaaccagcgc ggaaagccgg taaaaccggg ggcaacgcca
     2581 ggtaagtaag cggctactaa ccataccgcc agatacaaaa gggtcagccc cagcgcccag
     2641 cgcgcctctt tatgggcctg aacaaaacga gtgtccattt tttgtccctg atgggtgaag
     2701 aaagcgagga ttgtacctta tgggggttgt cgatccccag taataaaaaa ggccggaaaa
     2761 tccggccttt tgacgctttt gcagtcttat ttttcctgaa gaccgagttt tttctccaga
     2821 tagtggatgt tagtgccacc atgctggaag ttctcgtcat tcatgatgcg gatctgcaga
     2881 tcaacgttgg ttttgatacc gtcgatgatc agctcctgca gcgcattctt catgcgggca
     2941 atcgccacgt cacggttttc accgtagcaa atcagcttac cgatcattga gtcatagtac
     3001 ggcggtacgg tgtagcccgc gtagatatga gactcccaac gtacgccaaa accgccaggt
     3061 gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg tgttcggatc ttcggcgtta
     3121 atacggcatt ccaccgcatg gccgcgaaca tgaacctctt cttgcttgat cgacagcggt
     3181 tgaccggcag cgatacgcag ctgttctttg atcaggtcaa cgccggtgat catttcagta
     3241 accgggtgtt ctacctgaat acgggtgttc atttcgatga aatagaactc gccgttttcg
     3301 aacaggaact cgaaagtacc tgcaccgcga tagccgatat caacacacgc tttagcgcaa
     3361 cgttcgccga tgtagcgacg cagttccggg gtaatgcccg gtgctggtgc ttcttcgacc
     3421 actttctggt ggcggcgctg catggagcag tcacgttccg ccagatagat agcgttgccc
     3481 tgaccgtcag ccagtacctg aatctcgacg tggcgaggat tttccaggta tttctccatg
     3541 taaaccatat cgttgctgaa agcagctttc gcttccgcac gggtcatgga gatggattgt
     3601 gccagttcag cgtcgccacg cactacgcgc ataccgcgac caccgccgcc gccggaggct
     3661 ttgataatca ccggataacc aatgcgttta gcaatggcgc ggtttttatc catatcgtcg
     3721 cccagcgggc cgtcagaacc cggtacgcaa ggaacgcccg cttttttcat cgccgcgatt
     3781 gctgatactt tatcgcccat caggcgaatg gtttctgctt tcgggccgat gaagataaag
     3841 ccggagcgtt caacctgctc ggcaaagttt gcgttctcgg agaggaagcc gtaccccgga
     3901 tggattgcta ctgcgccggt gatttcagcg gcgctgatga ttgccgggat gttcagataa
     3961 ctttttactg acggagcagg gccgatacag accgtttcat ctgccagtaa tacgtgtttt
     4021 agatcgcgat ccgcgctgga gtgcacagcg acagtcttga tgcccagttc tttacaggca
     4081 cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa caattttatc cagcatgttc
     4141 gcctcgttac tcgatgacga ccagcggctc gtcaaattct accggttgtc cactttcgac
     4201 cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca ttttcatggc
     4261 ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga tgaacgcttt
     4321 tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggaacgta cgatgtgacc
     4381 actgatttcc gctgctgctg gcgcttccat ggaaggaacg gtcgccggag cggctgcgtt
     4441 agattgagct ggctgctgca tcattggtgc agcgtaagct tgttgcatca cagggaaact
     4501 tgcggcagga gctgcacggc taatgcgtac tgactcttcg ccttcagaaa tttccagttc
     4561 ggagatgcct gattcttcaa ccagctcgat cagtttttta atcttacgaa tatccatgag
     4621 tgggttccgt actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc gtctgtaaag
     4681 cgtatgcccc gacagctgta tgcatagcga taaattccag caggccggga cgccggtcta
     4741 ttttgcccca gagtgtcaat gttagacttg acggacattg tgcagtgatc tgctgctatc
     4801 ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt caccttttcc gcagcgcaaa
     4861 aactgcgtca gggaggacga ggtcgcacat tataacgatt tcgtagcaat tggcagctaa
     4921 atactggtct tatcagggaa gataatcaac agctaacatg taaataacct tcaacaccgt
     4981 gtaatttgca acaagccgca caattcacga aattagcgcc atcatcgacg gaacttctta
     5041 taacgtaagg tgaaaagctg taaagccagc cctgcgaaga tgaggggctg caatgagata
     5101 atcttcacag accacaaata atgtagggat gccaggatcg cgacaagata gacgacatta
     5161 cgcagcggtt gctggggatt acccaatttt cactgcatcg tctggataga tgtgaatgct
     5221 aaagcacgca aaattaaccg attgacaatg ctcagcatta aataggttcg agtgattaat
     5281 ttgtagtcaa acaaaatcag attattcacc ccattttgca gaaggtaaac tgttttgtca
     5341 ggcggcagtg cggctatctg atgctttcag aatttgccac ctgacaataa aaaagccgat
     5401 ttggtagcaa aatcggcttc ggtgagtttg cagcattcat cacatactcc gacaagcctt
     5461 ttcaagcatg acggaggttg cgttaatgca aggtttctac ccagggataa aagtgaccat
     5521 tgcggcggat gaatatctgt gttgtcagag gatggtcatc ctcacggaag cattgttctg
     5581 ttagctgtcg taatgcgctg agatttgctg gctgatcatt gtgggtgaaa aacagttttc
     5641 ctctggaggg aacggtcact aacaggtcgc cgcattcagg atcgggtgat tgcacccaaa
     5701 gatcgtcaat gagcatcaga ctgggctcat aagtaccccc tgcggagata acggcaactt
     5761 ttccatcgtt tgcgcctgta tcaacgattt cagccgtaac ttccggcaat atttgcttca
     5821 aattactgat ggcttgttcg cggtcaatgg tcacttcgta gcgcatcaga tagcgcatgg
     5881 tttcgctacc atcttcagca aacaagataa ttaactcatc gttaaagtct tcccaggcaa
     5941 aatccagcga ggcgttgagg acctctggat cagactcata ctggctaagc tgttggcgaa
     6001 cggtatccag ccaggctttt tctttaatca ccggaacaat acagcgcccg gtcggcagca
     6061 tttctgcaag ctgtttcagc atatctgcag ccacttcttg cagcgggtct ggtgcggctt
     6121 gataacgttt gtaggcatca tcaagggtga tttccaccgt ttggtgaccc gtatcaatgc
     6181 gcatcaattt atggtcagtc gaaatcatga ctttccagtt tggattatgt tgtacaactt
     6241 cctgaccaag atgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga
     6301 ataatgattt caaaatgagt tccctctctt attattcctt gctaaatggt tagttaacct
     6361 tcaccagcgt gcgaccctgg atctggttat taatgatggc ctcggcaaac ttcggtgcct
     6421 ctgccagaga tatctctttt gccgcctggg tatagaatga ttccggtaaa tcggcgacca
     6481 gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat tactgaatcc accccttgca
     6541 aacggacatt acgcagaata aatggcatga ccgtggttgg cagagtaaaa ccacccgcca
     6601 gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc cagcactttt gccagcactt
     6661 tgtcgccaac ggtgtcaatt gccccagccc agacttgttt ttccagagga cgggattcgg
     6721 caaactcatc acgagggaga atacggctgg cacctaaact tttcagatat tcatgcgtac
     6781 tttcgcgacc ggaaacggca acgacctgat aacccaactt atgcagcagc gccacggcgg
     6841 tactgccgac gccaccactg gcacccgtca cgacaatctc cccgtcctgc gggcgaacac
     6901 cggcatcttc cagcgccatc acacacagca tggcggtaaa accggcagta ccgataatca
     6961 ttgctttacg cgcgtccagc ccttgcggca tggcaaccag ccagtcacct ttcactcgcg
     7021 cctgctccgc cagcccaccc cagtggtttt caccaacgcc ccagccagtg agtaacacct
     7081 cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac agttccggca aaatcgatcc
     7141 caggaatcat cggaaaatta cggatgattt ttcccttacc ggtaatcgcc agcgcatctt
     7201 tatagttcag gctcgaccag tgaacatcga ccgtgacatc gccctccggc aggcgacttt
     7261 cgtccagagt ctgtactgat gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct
     7321 gcataagtgg tcctcatgtg catgatggat tggaaaataa tttctgaaga ctatactcgc
     7381 taatggaaaa gcaaagccga tgaagcgcaa taaattgcga gataaatctg atttgctagt
     7441 atgcccgctt cctcactatc ggagttaaca caaggatgag attaacgacg aaattttcgg
     7501 cctttgttac gctgctcacc gggttaacaa tttttgtgac tttgctgggc tgttcgctaa
     7561 gtttctacaa cgccattcag tataagttta gtcatcgcgt tcaggcggtg gcgacggcga
     7621 ttgataccca ccttgtgtcg aatgacttca gcgcattaag gccacaaatt accgaattaa
     7681 tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg tgataaacag gtttataccc
     7741 tggccagaaa tggtagttat cgtccggttg gcaccagcga tctgtttcgt gaactgagcg
     7801 ttccgttgat aaagcatccg gggatgtcgc tgcgtatggt ttatcaggat ccgatgggca
     7861 actatttcca ttcgttgatg accaccgcgc ccctcacggg ggcgattggc tttatcattc
     7921 ttatgctctt tctggcggta cgctggttac aacggcaact tgccgggcaa gaattgctgg
     7981 aaacccgggc tactcgtatc ttaaacggtg aacgtggctc taatgtgttg ggaaccatct
     8041 atgaatggcc gcccacaacc agcagtgcgc tggatacgct gcttcgtgaa attcagaacg
     8101 cacgcgaaca acacagccgt cttgatacgc tgatccgctc ttatgccgcc caggacatga
     8161 aaaccggcct caataaccga ctctttttcg ataatcaatt agcaacgtta ctggaagatc
     8221 aggagaaagt aggtacccac gggatcgtga tgatgattcg tctgccggat ttcaatatgt
     8281 tgagcgatac ctgggggcac agccaggttg aagaacagtt cttcactctg acgaatctgc
     8341 tatcgacatt tatgatgcgc taccctggcg cactgctggc gcgttaccac cgcagtgatt
     8401 ttgctgcgtt gttaccgcac cggacgttaa aagaggctga gagcatcgcc ggtcagttaa
     8461 tcaaagccgt cgataccttg ccgaacaata aaatgctcga tcgcgacgat atgatccaca
     8521 ttggtatctg cgcctggcgt agtggtcagg ataccgagca ggtaatggaa catgcagagt
     8581 ctgccacgcg taatgcggga ttgcagggcg gcaatagctg ggctatttac gatgactcgt
     8641 tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc
     8701 gcggcggccc gcgcctttat caaaaaccgg cggttactcg cgaaggtcag gttcatcatc
     8761 gcgaactcat gtgccgcatc ttcgatggta atgaagaggt tagctcggcg gagtatatgc
     8821 cgatggtctt gcagtttggc ttatcggaag agtatgaccg tctgcaaatc agccgtctta
     8881 ttccactatt gcgttactgg ccagaggaaa atctggcgat tcaggttacc gttgagtcgc
     8941 tgattcgccc gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcac
     9001 aacgaaaacg cataattatt gaacttgcag aggccgatgt aggtcaacat atcagtcgtt
     9061 tacaacctgt tattcgttta gtgaatgctt taggggtacg ggtagccgtc aaccaggctg
     9121 gtttgacgct ggtaagcacc agttggatca aagaacttaa tgttgagtta ctcaagctcc
     9181 atccggggct ggtcagaaac attgagaagc gaacggagaa ccagctgctg gttcaaagcc
     9241 tggtggaagc ctgctcaggg accagcaccc aggtttacgc caccggtgtg cgttcgcgaa
     9301 gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg gcaaggggat tttttcgcgt
     9361 cctcacagcc acttgatact aacgtgaaaa aatattcaca aagatactcg gtttaacctg
     9421 ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc
     9481 tgctcgccag attgttgcag cacatatgca gatgaatgac cttacgcggt tgcaaacagg
     9541 cgaggaatgc tgctgatgca ttaagccttt ctggactcag gcagagattt gtaacaaagg
     9601 aaacgaactg cactaatttt caccgtagca gatgattttt gcgccttgtc gctgctgcgt
     9661 gtggttggta aagtaagcgg attttctttt ccgccccagc tttcaggatt atcccttagt
     9721 atgttgaaaa aatttcgtgg catgttttcc aatgacttgt ccattgacct gggtactgcg
     9781 aataccctca tttatgtaaa aggacaaggc atcgtattga atgagccttc cgtggtggcc
     9841 attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag
     9901 cagatgctgg gccgtacgcc gggcaatatt gctgccattc gcccaatgaa agacggcgtt
     9961 atcgctgact tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc
    10021 aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg taccggttgg cgcgacccag
    10081 gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg
    10141 attgaagaac cgatggctgc cgcaattggt gctggcctgc cggtttctga agcgaccggt
    10201 tctatggtgg ttgatatcgg tggtggtacc actgaagttg ctgttatctc cttgaacggc
    10261 gtggtttact cctcttccgt gcgcattggt ggtgatcgtt tcgacgaagc tatcatcaac
    10321 tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac
    10381 gaaatcggtt cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac
    10441 ctggcagaag gtgttccacg cggttttacc cttaactcca acgaaatcct cgaagcactg
    10501 caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg
    10561 gaactggctt ccgacatctc cgagcgcggc atggtgctca ccggtggtgg cgcactgctg
    10621 cgtaaccttg accgtttgtt aatggaagaa accggcattc cagtcgttgt tgctgaagac
    10681 ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga catgcacggc
    10741 ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg gaagcgtctg tttaccctgc
    10801 ctggtctgat acgagaatac gcataactta tgaagccaat ttttagccgt ggcccgtcgc
    10861 tacagattcg ccttattctg gcggtgctgg tggcgctcgg cattattatt gccgacagcc
    10921 gcctggggac gttcagtcaa atccgtactt atatggatac cgccgtcagt cctttctact
    10981 ttgtttccaa tgctcctcgt gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg
    11041 accaattaga acttgaaaac cgggcgttac gtcaggaact gttgctgaaa aacagtgaac
    11101 tgctgatgct tggacaatac aaacaggaga acgcgcgtct gcgcgagctg ctgggttccc
    11161 cgctgcgtca ggatgagcag aaaatggtga ctcaggttat ctccacggtt aacgatcctt
    11221 atagcgatca agttgttatc gataaaggta gcgttaatgg cgtttatgaa ggccagccgg
    11281 tcatcagcga caaaggtgtt gttggtcagg tggtggccgt cgctaaactg accagtcgcg
    11341 tgctgctgat ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc
    11401 gcgtaattgc agccggtaac ggttgtacgg atgatttgca gcttgagcat ctgccggcga
    11461 atacggatat tcgtgttggt gatgtgctgg tgacctccgg tctgggcggt cgtttcccgg
    11521 aaggctatcc ggtcgcggtt gtctcttccg taaaactcga tacccagcgc gcttatactg
    11581 tgattcaggc gcgtccgact gcagggctgc aacgtttgcg ttatctgctg ctgctgtggg
    11641 gggcagatcg taacggcgct aacccgatga cgccagaaga ggtgcatcgt gttgctaatg
    11701 aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt
    11761 tacctgaacc ggcaacgggg atcgctcagc cgactccgca gcaaccgacg acaggaaatg
    11821 cagctactgc gcctgctgcg ccgacacagc ccgctgctaa tcgctctcca ccaagggcta
    11881 cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag
    11941 ctatcgtagc cagggacgct gggtaatctg gctctctttc ctcattgcgc tgttgctgca
    12001 aatcatgccc tggccggata acctgattgt tttccggcca aactgggtgt tactcatctt
    12061 gttgtattgg atcctggcct tgccccatcg cgtaaatgtg ggcacaggtt ttgtgatggg
    12121 tgccatactg gatctgatca gcggctcgac gcttggcgta cgcgtattgg cgatgagcat
    12181 cattgcttac ctggtggcgc tgaaatacca gcttttccgc aacctcgcat tatggcagca
    12241 ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt attgttttct gggcagagtt
    12301 tttagtgatt aacgtctctt tcagaccgga agtgttctgg agtagtgtag tcaatggggt
    12361 gctctggccg tggattttct tgctgatgcg taaagtccgt cagcagtttg cagtgcaata
    12421 aaggtttcta tgacttctct gtatttagct tccggttctc cgcgtcgtca ggagttactt
    12481 gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca ttgaggagca gcgtcagccg
    12541 caggagagcg cgcagcagta tgttgtgcgt ctggcgcgcg agaaagcacg ggcaggtgtc
    12601 gcgcaaacgg cgaaggatct cccggtgctg ggtgcggata ctatcgttat cctgaacgga
    12661 gaagtgctgg agaaaccgcg tgacgcagag catgcggcgc agatgttgcg caaattatcg
    12721 agtcagaccc atcaggtgat gacggcagtg gcgttggccg acagccagca cattctcgat
    12781 tgcctggtga tcaccgatgt gactttcaga acgttaacag acgaagacat cgcgggctat
    12841 gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg gtattcaggg gctgggtggc
    12901 tgttttgtca ggaagataaa tggcagctat cacgccgtag tcggtttacc gctggttgaa
    12961 acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga aaagggataa acatgacggc
    13021 tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg atggcggtat
    13081 tctgcaggaa attcatattg aacgtgaggc gcgacgcgga atagtaggca atatctacaa
    13141 gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct tttgtagata ttgggctgga
    13201 taaagccgcg tttcttcatg catctgacat catgccgcac accgaatgtg tggcgggtga
    13261 agaacaaaag caatttacgg tgcgcgacat ctcggaactg gttcgtcagg ggcaagatct
    13321 gatggtgcag gtggtgaaag atccgcttgg cactaagggg gcgcgcctga ccaccgatat
    13381 cacgctgcct tctcgctatc tggtctttat gccaggggct tctcacgttg gtgtttccca
    13441 acgtattgaa agcgaatcag aacgtgaacg cctgaaaaaa gtggtcgcag agtattgcga
    13501 cgagcagggc gggtttatca tccgtaccgc agcggaaggg gttggcgagg ctgaactggc
    13561 ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt atggagcgta aaaagcgccc
    13621 gcagacccgt tatcagctgt acggcgaact ggcgctggcg cagcgtgttc tgcgtgattt
    13681 cgccgatgcc gaactggacc gcattcgcgt tgactcacgc ctgacttacg aagcgttact
    13741 tgagttcacc tcggagtaca ttcccgagat gaccagcaag ctggagcatt acaccggacg
    13801 ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc cagcgagcgc tggaacgcaa
    13861 agtagaactg aaatccggtg gttatctcat tatcgaccag accgaagcga tgaccaccgt
    13921 ggacatcaat accggagcgt ttgtcggtca tcgcaatctg gacgacacca ttttcaatac
    13981 caatattgaa gcgacgcagg ctatcgctcg ccagttacgg ttgcgtaatc tgggcgggat
    14041 tatcattatt gatttcatcg atatgaataa tgaagatcac cgccgccgag tgctgcactc
    14101 gctggagcag gcgttgagca aagaccgggt gaaaaccagc gttaatggtt tttcggcgct
    14161 ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa
    14221 cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga
    14281 aatcatgcgc gagattgttc gtgtccacca tgcttacgac tccgaccgtt tcctggtcta
    14341 tgcttctccg gcagtagctg aagccttgaa aggcgaagag tcacactcgc tggcggaagt
    14401 ggaaattttc gttggtaaac aggttaaagt acaaattgaa ccgctctata accaggagca
    14461 gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca tccggcaaag ggtttttgag
    14521 tcacatattt agcagacaag gagtgacggg tgaggcgatt gccggggatt ttactgctta
    14581 ctggagccgc gctcgttgtg atcgctgccc tgctggttag cggcctgcgt attgctttac
    14641 cgcatcttga cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg actggcatgc
    14701 cggttgaagc cagtcagctc tcagccagct ggcagaattt tggcccgacg cttgaagcac
    14761 acgacatccg tgcagaacta aaagatggcg gcgaattttc ggttaaacgc gttactctgg
    14821 cgctggatgt ctggcagagc ctgttacata tgcgctggca gtttcgcgac ctcactttct
    14881 ggcagctgcg ctttcgcacc aacactccta tcaccagcgg tggtagcgat gacagtctgg
    14941 aagccagtca catcagcgat ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca
    15001 gtgaagtcag tttcctgacg ccatccggtc agcgcgccga gctggcgatc ccacaactca
    15061 cctggctgaa cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc tccagcctta
    15121 ccggacagca cggcgtgatg caggtgcgca tggatttgcg cgatgatgag gggttgttaa
    15181 gcaatggtcg cgtctggctc caggcggatg acatcgacct gaagccgtgg ctcggtaaat
    15241 ggatgcagga caatattgcg ctggaaacgg cacagttctc ccttgaaggc tggatgacga
    15301 tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa acagggcggt gccagctggt
    15361 tgggcgagaa gcaaacgcat acgctgtcgg tggataatct gaccgcgcat attacgcgtg
    15421 aaaatccggg ctggcagttc tctattcccg atacacggat cacgatggac ggcaaaccct
    15481 ggccgagcgg agcattgacg ctggcctgga taccggaaca ggacgttggc ggcaaagaca
    15541 ataaacgcag tgacgaactc cggattcgcg ccagtaatct ggagctggca ggcctggagg
    15601 gcatacgccc gctggccgcg aaactttcac ctgcactggg tgatgtttgg cgctccacac
    15661 aaccgagcgg caagattaac actctggcgc tggatatccc gcttcaggcg gcagacaaga
    15721 cccgttttca ggcatcgtgg agcgatctgg cctggaagca atggaaatta ttaccgggtg
    15781 cggaacactt ctccgggacg ctttccggca gcgtagaaaa tggtttgctt accgcgtcga
    15841 tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc gccactggaa atcgccgacg
    15901 gccaggcaac tataagctgg ctgaacaatg acaaaggttt ccagctggat gggcgtaata
    15961 ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt tcgttacctg caacctgcta
    16021 acgatgaacc ctggctgggt attctggctg gcatcagtac cgatgatggt tcacaagcct
    16081 ggcgctattt cccggaaaac ttgatgggta aagacctggt tgattactta agtggcgcga
    16141 ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg tggcaatccg caactcttcc
    16201 cctataaaca caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg
    16261 ccttccagcc ggactggcct gcattaacta accttgatat tgaactggac tttattaacg
    16321 acggtttatg gatgaaaacc gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca
    16381 ccgcagtgat ccctgactac tcgacagaaa aactgctgat tgacgctgac attaaaggtc
    16441 cgggtaaagc cgttggccct tactttgatg agacaccgct gaaagattct ctgggtgcgc
    16501 ccctgcaaga actccagctc gacggcgatg tgaatgctcg cttacatctt gatatcccgc
    16561 tgaacggcga gctggtaacc gcgaaaggtg aagtgacgct gcgtaataac agtctgttta
    16621 tcaaaccact cgacagcacc ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg
    16681 atctgcaaag tgaaccactg acagcaagct ggtttaatca gccgttgaac gtggattttt
    16741 ccaccaaaga aggggcaaaa gcctaccagg tagcggtaaa cctcaacggt aactggcaac
    16801 cggcgaaaac cggcgttctg cctgaagcgg tgaacgaagc attgagtggc agcgtggcgt
    16861 gggatggtaa agtgggcatt gagctgcctt atcatgctgg cgcgacctat aacgtagagc
    16921 tgaacggcga tttgaagaat gtgagcagtc acttaccttc accgttagcc aaacctgcgg
    16981 gtgaaccact agcggtaaac gttaaggttg atggcaatct caacagcttt gaattaaccg
    17041 gacaggctgg tgcggataat catttcaata gccgctggtt gctcggtcaa aagctcacgc
    17101 tcgaccgtgc tatttgggcg gcagacagta aaacgctccc gccgttgccg gaacaaagtg
    17161 gcgttgaact caatatgccg ccgatgaatg gtgccgagtg gctggccctg tttcagaaag
    17221 gtgcggcgga gagtgtcggt ggtgcagcga gtttcccaca acacataacg ttacgtacgc
    17281 ctatgttgtc gctgggaaat cagcaatgga ataacctgag tattgtttcg caaccgacgg
    17341 caaatggcac ccaggttgag gcgcaagggc gtgaaatcaa cgccacgctg gcgatgcgta
    17401 ataacgcgcc gtggctggcg aatatcaaat atctttatta caacccgagc gtggcgaaaa
    17461 ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac ggagcgcatt aacttccgtg
    17521 gctggccgga cgcacaaata cgatgcgcag agtgctggtt ctgggggcaa aaattcggtc
    17581 gtattgacag tgatctcacc atttctggcg atacattaac gctgaccaat ggactgattg
    17641 atactggttt ttcgcggctc actgccgatg gtgaatggat taacaatccg gggaatgaac
    17701 gtacctcgct gaaaggaaaa ctgcgcgggc agaaaattga tgccgccgca gaattttttg
    17761 gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta cactggcgca
    17821 aagcaccgtg gcagccggat gaggcgacgt tgaatggcat cattcatact caactgggca
    17881 aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc ttattgagcg
    17941 tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc gaagggttct
    18001 attttgactc cattcgcagc accgcgtgga ttaaagacgg cgttatgcac accgacgaca
    18061 cgttggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat ctggtacgtc
    18121 gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg ggcgtggctg
    18181 cggcttttgc ggttaacccc attgttggcg cggcagtgtt tgccgccagt aaagtgctgg
    18241 ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg ctggacgatc
    18301 cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga tttgacgagg
    18361 gcgcgtaatt gccccaatct cataggataa tcgttgccaa aggccaacga gccagaacat
    18421 aaccgtaggt cggatagggc gttcacgccg catccggcag ccgtaaaaaa tcctctactg
    18481 cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac aattgctagc
    18541 ggcgaacggc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg
    18601 ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg ttttagaaga
    18661 ccgtattatt aaagatggtt cttacaacat cgatcagggc gttggtgtgc gtgcaatcag
    18721 cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc tggaacagag
    18781 tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggtaaagtac agacgctggg
    18841 cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca tgagccgtga
    18901 agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg acaagcgcgt
    18961 acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg cggccaccga
    19021 cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg ttctggtcga
    19081 agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg gttatgagtt
    19141 cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag aagcagtgcg
    19201 tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact
    19261 tggcgcaggt tggccgggcg tgctgttgca tgaagcggtt ggtcacggtc tggaaggcga
    19321 cttcaaccgc cgtggcactt cagtatttag tggacaggtc ggggaactgg tggcttcaga
    19381 actgtgtacc gtggttgatg atggcacgat ggtcgatcgc cgaggttcgg tggcgattga
    19441 tgacgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc tgaaaggcta
    19501 catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca acggtcgccg
    19561 tgaatcctac gcccatctgc ccatgccgcg tatgaccaac acctatatgc tgccgggtaa
    19621 atcgaccccg caggaaatta ttgaatccgt tgagtacggt atctatgcac cgaactttgg
    19681 tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag aagcatatct
    19741 gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg gttccggtat
    19801 cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata acggcgtggg
    19861 tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt
    19921 tgataacctg actgttggcg gtactgcgta ataatttcag tattttcaga aggataatta
    19981 atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga ggggaacccg
    20041 ttggcacagg tttgtacaac gtaacagtac aatatgaatt acttctcttt cccgcgcccg
    20101 tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga gttgatcacg
    20161 acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac cgggcgtgga
    20221 tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc gttgatcacc
    20281 cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac cagcgtcatc
    20341 ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc ttccggtgca
    20401 atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg gctacttaaa
    20461 tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc gcacaccacc
    20521 attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg
    20581 atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt aaccagattg
    20641 acgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag aacattttgt
    20701 gccatagttg aagaacagcc aatgcgtagc gttccgatgg gggtgttatt gaaggcatac
    20761 agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg gtagtaaatt
    20821 ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt tacctgcaac
    20881 tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg tagctgtctg
    20941 gcggcggcgg taaaagagcc aaattcaact actttggcaa ataccgacat gcgttttagt
    21001 cgttccatta ttcactctga cttaaaagtg atttagatca
//
LOCUS       DAZUGK010000069        20997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZUGK010000069 DAZUGK010000000
VERSION     DAZUGK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.069"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..1381
                     /gene="amiC"
                     /locus_tag="ACHY5W_002213"
     CDS             128..1381
                     /gene="amiC"
                     /locus_tag="ACHY5W_002213"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFS4447107.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(1456..1532)
                     /locus_tag="ACHY5W_002214"
     tRNA            complement(1456..1532)
                     /locus_tag="ACHY5W_002214"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1496..1498),aa:Met,seq:cat)
     gene            1741..2838
                     /gene="mltA"
                     /locus_tag="ACHY5W_002215"
     CDS             1741..2838
                     /gene="mltA"
                     /locus_tag="ACHY5W_002215"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFS4447108.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            3076..3882
                     /gene="tcdA"
                     /locus_tag="ACHY5W_002216"
     CDS             3076..3882
                     /gene="tcdA"
                     /locus_tag="ACHY5W_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFS4447109.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3933..4376)
                     /gene="csdE"
                     /locus_tag="ACHY5W_002217"
     CDS             complement(3933..4376)
                     /gene="csdE"
                     /locus_tag="ACHY5W_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFS4447110.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLVLFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(4376..5581)
                     /gene="csdA"
                     /locus_tag="ACHY5W_002218"
     CDS             complement(4376..5581)
                     /gene="csdA"
                     /locus_tag="ACHY5W_002218"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFS4447111.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDIVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5773..6000
                     /gene="ygdI"
                     /locus_tag="ACHY5W_002219"
     CDS             5773..6000
                     /gene="ygdI"
                     /locus_tag="ACHY5W_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFS4447112.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6351..7268
                     /gene="gcvA"
                     /locus_tag="ACHY5W_002220"
     CDS             6351..7268
                     /gene="gcvA"
                     /locus_tag="ACHY5W_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFS4447113.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7287..7682
                     /gene="ygdD"
                     /locus_tag="ACHY5W_002221"
     CDS             7287..7682
                     /gene="ygdD"
                     /locus_tag="ACHY5W_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFS4447114.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7675..8775
                     /gene="rlmM"
                     /locus_tag="ACHY5W_002222"
     CDS             7675..8775
                     /gene="rlmM"
                     /locus_tag="ACHY5W_002222"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFS4447115.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8819..9550)
                     /gene="fucR"
                     /locus_tag="ACHY5W_002223"
     CDS             complement(8819..9550)
                     /gene="fucR"
                     /locus_tag="ACHY5W_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFS4447116.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9608..10030)
                     /gene="fucU"
                     /locus_tag="ACHY5W_002224"
     CDS             complement(9608..10030)
                     /gene="fucU"
                     /locus_tag="ACHY5W_002224"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFS4447117.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(10032..11450)
                     /gene="fucK"
                     /locus_tag="ACHY5W_002225"
     CDS             complement(10032..11450)
                     /gene="fucK"
                     /locus_tag="ACHY5W_002225"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS4447118.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11559..13334)
                     /gene="fucI"
                     /locus_tag="ACHY5W_002226"
     CDS             complement(11559..13334)
                     /gene="fucI"
                     /locus_tag="ACHY5W_002226"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFS4447119.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(13367..14683)
                     /gene="fucP"
                     /locus_tag="ACHY5W_002227"
     CDS             complement(13367..14683)
                     /gene="fucP"
                     /locus_tag="ACHY5W_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFS4447120.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            15230..15877
                     /gene="fucA"
                     /locus_tag="ACHY5W_002228"
     CDS             15230..15877
                     /gene="fucA"
                     /locus_tag="ACHY5W_002228"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFS4447121.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            15905..17053
                     /gene="fucO"
                     /locus_tag="ACHY5W_002229"
     CDS             15905..17053
                     /gene="fucO"
                     /locus_tag="ACHY5W_002229"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4447122.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(17108..17863)
                     /gene="xni"
                     /locus_tag="ACHY5W_002230"
     CDS             complement(17108..17863)
                     /gene="xni"
                     /locus_tag="ACHY5W_002230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFS4447123.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQTGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(17975..19342)
                     /gene="sdaB"
                     /locus_tag="ACHY5W_002231"
     CDS             complement(17975..19342)
                     /gene="sdaB"
                     /locus_tag="ACHY5W_002231"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFS4447124.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(19400..20689)
                     /gene="sdaC"
                     /locus_tag="ACHY5W_002232"
     CDS             complement(19400..20689)
                     /gene="sdaC"
                     /locus_tag="ACHY5W_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFS4447125.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5166 a         5173 c         5572 g         5086 t
ORIGIN      
        1 tgtcgtgaat ataaaaagaa tgtttaccgt ttattagatg acagattatg cgtctttcgc
       61 taaagtttcc ggtcaaatta gtcgtttact tgttacacag cttagatttc ttttgctcgg
      121 gagaggcatg tcaggatcca acactgcaat cagccgtcgt cgtttactgc aaggcgcggg
      181 tgccatgtgg ctattgagcg taagtcaggt cagtctggct gcggtcagcc aggtcgtggc
      241 ggtgcgcgtc tggcctgcgt ccagctacac ccgcgtgacg gtagaatcaa atcgtcagct
      301 gaaatataag cagttcgcgt tgagtaaccc ggaacgcgtg gtggtggata tcgaagatgt
      361 aaacctgaac tcggtactca aggggatggc ggcacaaatt cgtgcagacg acccgttcat
      421 caagtcggcg cgcgtcgggc aatttgaccc gcaaaccgta cggatggttt ttgaattaaa
      481 gcaaaacgta aaaccgcagc tgtttgccct tgcgccggtc gccgggttta aagagcgtct
      541 ggtgatggac ctctatccgg ccaatgcaca ggatatgcag gacccgctgc tggcgctgct
      601 ggaggattac aacaaaggcg acctcgaaaa gcaggtgcca ccagcgcaaa gtggtccaca
      661 accaggtaaa gcagggcgcg atcgtccgat tgtcattatg cttgaccctg gccacggtgg
      721 cgaagactcc ggtgcggtgg ggaaatacaa aacgcgcgaa aaagacgtgg tattgcaaat
      781 agctcgccgt ctgcgctctc tgatcgagaa agagggcaat atgaaggtgt acatgacgcg
      841 caatgaagac atcttcattc cgttgcaagt gcgcgtagca aaagctcaga aacagcgtgc
      901 tgacctgttt gtctctatcc atgccgacgc ctttaccagt cgccagccga gcggttcctc
      961 ggtgtttgcg ctctcaacca aaggtgcaac cagtactgcg gcaaaatatc tggcacaaac
     1021 ccagaacgcc tcggacttga ttggtggcgt gagcaaaagc ggtgaccgct atgtcgacca
     1081 caccatgttc gatatggtgc agtcgctgac cattgccgac agccttaagt ttggtaaagc
     1141 ggtgctgaat aagctcggta aaatcaacaa gctgcataaa aatcaagttg aacaggccgg
     1201 gtttgccgta ctaaaggcac cagatattcc ctccattctg gtcgaaacgg cgtttatcag
     1261 taacgttgag gaagagcgta aactgaaaac ggcgactttc cagcaggaag ttgcggagtc
     1321 tattcttgcg gggattaaag cgtattttgc cgatggggca acgctggcga gaaggggatg
     1381 ataaaaaggc gctgaatggc gcttttttta tgggagacca gaaacaaaaa aacacccgtc
     1441 agggtgttca ataattggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag
     1501 cccgacgagc taccaggctg ctccaccccg cgtccgtgga tgcgcactat actccgctcg
     1561 cgttttcatg caaccttttt tttaccccga tcacggattt agcatgattt tgaacaaaaa
     1621 caaaccacta aagcctattt tttgtgcaaa atttgcgtca gggagaggct gctcatttca
     1681 ttaaacgcgg cggtttgtta tcttcgttgc gccttatttt ttaacctgaa gaagagaaca
     1741 atgaaaggac gttgggtaaa gtaccttctt atgggcacgg ttgtggcaat gcttgccgcc
     1801 tgctcttcca aaccaaccga tcgcggacag caatataaag acgggaaatt tacccagcct
     1861 ttctctctgg tgaaccagcc agatgccgtt ggcgcgccga ttaacgccgg tgattttgcc
     1921 gagcaaatta accatatccg taattcgtca ccgcgtctgt atggcaacca gagtaatgtt
     1981 tataacgcgg tgcaagagtg gctgcgcgca ggcggtgata cccgcaatat gcgtcagttc
     2041 ggcattgatg cctggcagat ggaaggtgcc gacaactatg gtaacgtgca gtttaccggt
     2101 tattacacac cggtaattca ggcgcgccat acccgccagg gcgagttcca gtatcctatt
     2161 taccgtatgc cgccaaaacg tggtcgcctg ccgtctcgtg cggagatcta cgcgggggca
     2221 ttgagtgata aatatattct cgcttacagt aactccctga tggataactt cattatggat
     2281 gtgcagggta gtgggtatat cgactttggt gatggcagtc cgcttaactt tttcagctat
     2341 gcagggaaaa acggtcatgc ctatcgcagc attggtaagg tgctgatcga ccgtggcgaa
     2401 gtgaaaaaag aagatatgtc gatgcaggcg attcgtcact ggggcgaaac acacagtgaa
     2461 gccgaggttc gcgagctgct ggaacagaac ccgtctttcg ttttctttaa accgcaatct
     2521 tttgcaccgg tgaaaggggc aagtgcggtg ccgctggttg gtcgcgcttc agttgcctct
     2581 gatcgttcca ttatcccacc aggtacaacc ttgctggcag aagtgccgct gctggataat
     2641 aacggcaaat ttaacggtca gtacgaattg cgtctgatgg tggcgttgga tgtcggtggc
     2701 gcaatcaaag gccaacactt cgatatctat caagggatcg ggccggaagc cggacaccgc
     2761 gcaggttggt acaaccacta tggacgggtt tgggtgctga aaacggctcc gggcgcaggt
     2821 aacgtcttta gtggctgatg tgatattctg tatgccgttt ttacggatat gacagggtga
     2881 ggggtaacct cacatcctga ttattgacta agtgcgcgtt gttcatgccg gatgcggcgt
     2941 gaacgcctta tccagcctac aaatcatgca aatccaaaat attgcaggag ttatgtaggc
     3001 ctgataagcg tagcgcatca ggcatttatg cgtttagggt gagggataac ctcgcccttt
     3061 ttattctgag gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc
     3121 acagcgcgtc tgtatggtga aaaagcgctg caactgtttg ctgacgcgca tatttgtgtg
     3181 gtcggtattg gcggtgtcgg ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc
     3241 gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcat
     3301 gccctgcgcg ataacgttgg gctggcaaaa gcggaagtca tggcggaacg tattcgccag
     3361 attaacccgg agtgccgtgt aacggtggtg gatgatttcg tgacgccgga taacgtagcg
     3421 caatatatga gtgccggtta ttcgtacgtg attgatgcca ttgatagtgt acggcccaaa
     3481 gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg
     3541 ggtgggcaga ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggac
     3601 ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt
     3661 aaaggtaagc tcggcgtgga ttgcgtgttt tctactgaag cgctggtgta cccgcagtca
     3721 gacggtacgg tgtgtgcgat gaaagccacg gcggaaggac caaagcggat ggattgtgca
     3781 tctggatttg gcgcggcaac gatggtgacc gccacctttg gttttgttgc ggtttctcat
     3841 gcgctgaaga agatgatggc gaaagcggcg cgtcaggggt aaacaatcag gtcggatgcg
     3901 acgcaaacgt cttatccgac ccggttctgt gcttaaacct gcttcgtcgc agcgataatc
     3961 gcctcgctta acgcatttaa cccctggctg cgtgaggcgc taagttgcgc acgcaatccc
     4021 agctcatcaa acaataccag cggcgactgt gcctgcaatt cggcggcggt tttcccctca
     4081 acggcagtca acagcaccgc cagcaggccg cgcactatac gtccttcgct gtcgccaaag
     4141 aagtgcattt taccgttttc agccacggta tatcccagcc agacgcggtt ttcgcatccg
     4201 gcaatctctt tagcctgcgc ttttaactcg tctggcaaag ccggaagctg tttccccagc
     4261 atgatcaact ggcgatattt atcttcccat tgcgtcaacg gtgcgaaggt attgcgtaac
     4321 gtttctgcgg ttacggttgt gccgaacgga tgtccggcga attgcgggtt cgtcattaat
     4381 ccaccaataa ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg
     4441 tattatatgg cgcaaaagag gcgcgcagtg tgccggtcac gcctaattct gccagtagcg
     4501 gctgagcgca atgttgcccg gcccgcaagg caataccgta ctccgccagc agcgtcacaa
     4561 tatcgctgtg atgaacgcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga
     4621 atgaacggaa gcctggacgt ttcgccagcg catcttccgc cagtgttgct aaactacggc
     4681 tccagctttc ggcctggttg atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc
     4741 ctatgacacc agccacattt ggcgttcctg cttccagttt ccacggcgca gattgagtcg
     4801 tgaagccgtc aaaactcact tcgtgaatca ttttgccgcc gcccagccag ggcgacatcg
     4861 cttccagcag ttctgatttg ccatacagca cgccgatacc cgtcgggcca tacagtttgt
     4921 gaccagagaa agcatagaaa tcgatatcca gttgctgaac atccgcgggg aaatgcaccg
     4981 ccccctgagc accatcaacc atcaccacca tcccggctga atgagcaaag gtaatcgctc
     5041 gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg acccaacgcc agaatccgac
     5101 tacggggagt aatcagttct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg
     5161 gcaatttcac cactttggct ccagtttgtt gggcaaccat cagccagggg acgaggttag
     5221 cgtggtgttc tgccacgctg acaataatct catcgcccgg ttgcagacgc ggacgcgcat
     5281 agcattgtgc caccatgttg atggattcag tggtgccgcg cgtccagacg atagttttat
     5341 catccggtgc attcagtaat tgcgccactt tctctcgtgc agcttcataa cgcgcggtca
     5401 ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc ggcgctcaga ctgtaaaact
     5461 gtcgggtggc ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg tcgagataga
     5521 cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg cgcgggatta aaaacgttca
     5581 tggtactcct cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag
     5641 tgctaaccat cagaattatc tggatatcct ggcgaagccg cgtcagtaac atatgctgaa
     5701 tagtgttagg cgttaatttt agcctgttaa tcaatactat taagatttat taatcgcttt
     5761 aaggagaaga tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc
     5821 ctgagcgcct gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc
     5881 tctgacggca aaccacagac tgataacgat accggtatga tttcgtataa agacgctaat
     5941 ggcaacaaac agcagatcaa ccgtaccgac gtgaaagaga tggtcgaact ggatcagtaa
     6001 ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg
     6061 gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat
     6121 cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag
     6181 aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt
     6241 tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta
     6301 cacagtgtga tctaattgtt aaattcattt aacatcaaag tttaaaagcc atgtctaaac
     6361 gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttca
     6421 ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc
     6481 ttgaggattt tttggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg
     6541 aggaagggca aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga
     6601 cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac ggtcagttta ctccccagtt
     6661 tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg
     6721 acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg
     6781 cgatatttta tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat
     6841 atttattgcc ggtgtgttcg ccgctactgc tgactggcga aaaacccttg aagaccccgg
     6901 aagatctggc taaacatacg ttattacatg atgcttcgcg ccgtgactgg cagacatata
     6961 cccgacagtt ggggttaaat catatcaacg ttcagcaagg gccaattttt agccatagcg
     7021 ccatggtgct gcaagcggct atccacgggc agggagtggc gctggcaaat aacgtgatgg
     7081 cgcaatctga aattgaagcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta
     7141 aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct
     7201 ttcgtcaatg gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg
     7261 aacaataatt tacgtagggt acgaccatga ccagccgttt tatgctgatt ttcgccgcca
     7321 ttagcggctt catttttgtg gctctgggcg cttttggcgc gcatgtgtta agtaaaacca
     7381 tgggggccgt tgagatgggc tggatccaga ccggcctcga ataccaggcg tttcatacgc
     7441 tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat tagtatctgg ttttactgga
     7501 gtagcgtttt cctcgcgtta ggcacggtgc tgttcagcgg cagcctttat tgcctggcac
     7561 tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg cggcgtgagc ttccttgcgg
     7621 gctgggcgtt aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat
     7681 aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat
     7741 aaagccggcc agcgggaaat tttcggtttt gcccgcgtga aagagaatgc gggttatgtc
     7801 atttatgaat gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt
     7861 tcattaattt ttgcccgcca gtggtttgtg gtgggggaac tcttgcagca tttgccgcca
     7921 gaagatcgta ttacccccat tgtcggcatg ttgcagggcg tagtagagaa gggcggtgaa
     7981 ctgcgtgttg aagttgccga taccaacgaa agcaaagagt tgctgaaatt ctgccgtaaa
     8041 tttactgttc cgctacgcgc tgccttgcgc gatgccgggg tgctggcgaa ctatgaaacg
     8101 ccgaagcgtc cggtagtgca tgtattcttc attgcaccag gctgctgcta taccggttac
     8161 tcatacagca acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca
     8221 gatgcgccga gtcgttctac gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg
     8281 gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg tggatttggg cgcttgccca
     8341 ggcggctgga cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc
     8401 ccgatggcgc aaagtctgat ggataccggg caggtgacgt ggctacggga agacgggttc
     8461 aaattccgtc cgacgcgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg
     8521 gcgaaagttg cggcattgat ggcgcagtgg ctggttaatg gctggtgtcg tgaaaccatt
     8581 ttcaacctca aactgccgat gaaaaaacgc tacgaagaag tgtcacacaa tctggcgtat
     8641 attcaggcac agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat
     8701 cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt
     8761 cgcgacgagc gataacaggc aaggccgggg cgtcgtctcc ggcctgctac cctttccgtc
     8821 aggctgttac caaagaagtt gcaacctggc gctcatcaga aataatgtgc gttacctcat
     8881 ccagatgccc gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca
     8941 acgattgcgc ggcgcgtttt aatagcatcg atttgtaatc agcgttgatg gcattggagt
     9001 cccacagtgc gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca
     9061 gcgatttaag ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg
     9121 tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc gagttcatgg caaatcggat
     9181 ggctattggt gaagacctga atgttgatgt caggcaactg gcgtgccaga taccagcaag
     9241 ttgaactggc gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg
     9301 cgatatctgc tttatgcgca taatggcttt tcagcctgat gtgaaaggga tcgccactgt
     9361 cttgattttg acggtggata tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg
     9421 tctgtaattc attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga
     9481 gagcttccgt ggtcaggctg gtatggttca gcagcaggtc gactattgct tgctggcgtg
     9541 ccgctttcat atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg
     9601 tatgagatta cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc
     9661 ctgtgataac gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc
     9721 ggatgatgtc aggacagggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt
     9781 caggatcgag agtgtcacct tctaccgctg ccatcatcac cagcggcggt gcataactgt
     9841 ccagctcaaa taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc
     9901 ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat
     9961 ctccatgtcc catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg
    10021 ttttcagcat ttcacacttc ctctataaat tcaggttcag tttgcgggta gaaataacgg
    10081 tactgataat gaatctgtgc gcgggcttct tccgggctgt taaattcccc tacgccatac
    10141 caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt cgtcgagaac ttttaccgga
    10201 atatcaagca tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccgccgact
    10261 aacaataatt cagaggcttt aaagtggccg attttttcca gcatctgtag attgcgctgt
    10321 aattgcgcag ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc
    10381 gtcactcctt gccagccagc gttctgacac gacaataaat cacactgcat tttcacgcca
    10441 tccgcgccag gcgcgatcag acgagcctct tcaatcaaca tttgccaggg tgtttcagcc
    10501 gtccagaaca gttttctcac ccattccagc acgccggatg ccagccattg catacctggg
    10561 ttatacaacc ctgcctggct atccagttcg caggtggaac cggcgtactg acttaacagc
    10621 gaagtatcaa cctgggcgct gcgaaccatt aaaatttccc atgtaccgga agagagcacg
    10681 ggttcatttt gttcagcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa
    10741 atcaccggta tgccaacggg taagccgagc attgctgcgg cgctgttctg tagcgtacca
    10801 atctgttcac ccgcttccac cagacgaggg aagagtcggc gtggaatacc ggtggcttgt
    10861 aaaatttgcg gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc
    10921 gtgatatcag tagtgaattc gccggttaaa cggtggttaa tcagcgacga aataaagagc
    10981 caggcgtgtg cgcgttccag cagttgtgga tgattttctt tcaaccacac caatttatat
    11041 aacgtattga aactaaaggc tccgacacca gaaatagcct gcaaccgctg tgcggagatc
    11101 aactgttcaa tattgtccat aaccgctgct gttcgcggac atttccagct aatgatcgga
    11161 tagagcagat tgccttgctt atctaccaga gcgccatcca caccaaaggt ggtgacggcg
    11221 ataccgcgga tgtggcattc agtcagttca ctattgattt gccgacagca atcagcaaag
    11281 cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt tgttttccat cgcgatatcg
    11341 ctggcattag gcgttgaggc gcgggcaaca atttttccct gccgattaac cgcgatggcc
    11401 ctgacattgg tcgcgccaca gtcgagtacc aggataactt cttgtttcat aatcgctcct
    11461 gcaatatagc cggataatat tgcttatccg gctaaccact cttgcttgtt caggcccggt
    11521 agccggagcg accgggcatc acatcaggga gtaatgtatt aacgcttgta caacggcccg
    11581 tagttctggc aagcgcggta atcctggcct tcaatatcca tgccgtgcgc agcccaggca
    11641 gaaggacgat acactttggt ctcttcaacg ttgtgcatac ataccgggat acgcagcatg
    11701 gaggcgagag tgataaagtc tgcgccaacg tggccgatgg tcagaactcc gtggttggca
    11761 ccccagttcg ccattaccga atacacatcc gtaaacggcc ctttaccagt gaggcgcggt
    11821 gcaaaccagg tggttggcca ggttgagttg gtgcgtttgt tgaggatgtc atgcacatcc
    11881 ttcggcaatt ccacgctcca gccttccgcg atttgcagta ccggtcccag gcctttgatg
    11941 atgttgacac gagtcatggt gaacgggacg ccgccttcgg taaggaagcg ggaagagtaa
    12001 ccgccgccac ggaagtattc gtggatcgcc gggcaccatt cggtagcggc gaggcaagcg
    12061 tcagcctctt gctgagagat ttcccagtgt ggcttcatcg tcgggttacc ttcgctgtcg
    12121 cgttgtttac aggaaccgtc cagcgcagca gaaccggagt tgatcaaatg gatgatgccg
    12181 tgttctgcca gtccatccag tttatgcccc gttacacgct caattgcttc tggtgaccag
    12241 taggtacgca catcggcaaa tacctgagcg gtgccggtga gctggtgacc cattagcatt
    12301 gccacgccgt taagactgtc gttttcggtc gccacgacaa agggttcgcg cacgccattc
    12361 cagtcaaatg aactgttgag gatcgcttcg gcggtgtcac cattgggata ttgatcggtc
    12421 cagtgacgtt gcccctggaa gcccgcagcg atggcgttgt agccaagtga ttcttccacg
    12481 cgaccaatat cggccagttt gctgttgcct tgcatcatgt cgcggataca catcgccatc
    12541 agtaaacttt cgcgcagaac tgcgcggctt tgctcggcat tgcgttgata ctgtttgtta
    12601 ttttcatctt cgccatagcg gaagttttta tcagcccagg ccagtgccat ttccagttcg
    12661 gcttcgtcgt aaatcttctg atcgatacgg cgacgcagtt cggtcatatc caccgcctgg
    12721 actttcattc ccagccagga ttcaaagaag ttgtgatcaa caatagaacc ggcgataccc
    12781 atcgaaacgc cgcccagcga cagatagctt ttacctttca tgctggcgac ggccaaaccg
    12841 gcgcgggcaa agcgcagcag tttttcttca acatcggcag gaatcgatgt gtcatcggca
    12901 tcctgaacgt catgaccgta aatggagaat gccgggatgc ctttctggct gtgagctgcc
    12961 agagccgctg ccaggtaaac agcgccggga cgttcagtgc cgttaaagcc ccaaatggct
    13021 ttcgggcggg ttggatccat gtcgatggtt tcactgccat agcaccagca aggcgttacc
    13081 gtaatggtga ggcctacatt ctggctgctg aatttttctt cgcaagcagc ggcttcagcc
    13141 atacccgcga tacaggtatc ggaaatgaca cactcgacgg cagctccgca ggcatggcgc
    13201 agtttctcgg tcagcagtgc ggccgtagct ttcgccatat tcattgtttg ttcttcaagc
    13261 gactcacgaa cacccatgcg acgaccgtca ataaccgggc ggataccaat tttcggtaag
    13321 ctgatttttt tcattacaga ttcctcactt tattcggaaa atatgttcag ttagttgccg
    13381 tttgagaacg gaaacgggca aagataaaga tgaccgcgaa gcagagtgcg gggatcagtt
    13441 cagcagtggg gatgttgccc gccgcgtcac tgacaaaacc catgaccgga gtgacaatac
    13501 cgccgccaat aatggtcata acgatgaagg acgaaccgta tttggtgtcc tggccgagat
    13561 tcttaatgcc cagcgagaag attgttgggt actgaatcga cataaaggcg ctgcataaag
    13621 tcagggctat taagcccaca tgaccgccag cgaaggctga gatcaggcac agtgccatag
    13681 cgattaatgc gtaggcggcc aggactttgt gtggtgcgaa gcgactgatg agccaggtac
    13741 cggtgaaacg accaataaag aagcacacca tggttccggt taaatagtta gcggcaaagc
    13801 ctgcagtcat acctggaatt tcttctacag cgtagcgaat caaatagctc cagcaggccg
    13861 tttgtgcgcc aacgtagcag aattgcgcta atactgccca gcgccagtgg cgaatacgcg
    13921 ccaggcgaga aagcgatgcg gagaacgatc cttgtttggc gtcactgtgg ttatcactct
    13981 gcaatgccgg gaatttcgtc agcatgatca gcagggcgac cagtaacacg atagccacga
    14041 tgatcatata aggtgtctgt accgataata ccaggctgtg tttatacgca ctcaattgct
    14101 ctggagacat tttatcgaga acgtcttgcg attgatgtgg cacgttagac aaaataagac
    14161 tttgcccaaa gacaaccgcg ataattgcgc caaatgagtt aaatgtttgc gcaagattta
    14221 agcggaagtg accactgctt tccggcccta ataccgtaac aaaagggttt gcggcagttt
    14281 ccagacaacc taatccggct gcaataataa ataggccaac taaaaataag gtgtagttca
    14341 ttatttctgc ggcgggccag aataatgcag cacccaaggc atataaaaat aacccggtaa
    14401 taattcctgc tttataactg agttttttca tcaatatccc agcaggaatt gggataatga
    14461 aataaccaaa gtaaaaggcc gattggatca ggccagcctg gaaatttgtc agcgtaaaag
    14521 cctgctggaa ttgaggtaat aaaatgtcgt taaggttatt ggctaccgcc caaagaaaaa
    14581 acagtgagca cagcagcgcg aatggaataa tgtaacttct gctttgccct gcatctttat
    14641 ctaccgcacg gtaactctgc gtttgtattg atgtgtttcc catagcatcc tcttaggttc
    14701 agaagcttaa gcacgtaatc ggttatgttt tcaaaagtgc aaaaccagcg agttcgggct
    14761 tcatatcagt gaatatttgc ctgtctgaaa tgaactcatg gattcatttt caaataaaaa
    14821 acgctctgta atatgacggc ggtcacactt attagctgga aggtgacttt atgtgactac
    14881 catcacttta attatcgaaa tttcatgccc gtttcagaaa ataaatcata aaaaacacaa
    14941 accagatgac cgtattaatt gtttgtgccg aaaacgggca ttcctggctt gggtgaatag
    15001 aaaaatgacc gttttgataa atatttttaa gagcggtcat ttttaatggg gttataaaaa
    15061 gaaaacttgc agttatttac tgtgattagt tgaaccaggt cacaaaatat aagcttgtga
    15121 atttctttct ggttaatcag gaagaagatg gtgggtaatt aaacggctaa ttcaatagtg
    15181 tgaaaggaac aacattattg ccctgttttg aatcagagag aggtagctaa tggaacgaaa
    15241 taaacttgct cgtcagatta ttgacacttg cctggaaatg acccgcctgg gactgaacca
    15301 ggggacagcg gggaacgtca gtgtacgtta tcaggatggg atgctgatta cgcctacagg
    15361 cattccatat gaaaaactga cggagtcgca tattgtcttt attgatggca acggtaaaca
    15421 tgaggaagga aagctcccct caagcgagtg gcgtttccat atggcggcct atcaaagcag
    15481 accggatgcc aatgcggttg ttcacaatca tgccgttcat tgcacagcag tttccattct
    15541 taaccgaccg atccccgcta ttcactacat gattgcggcg gctggcggta attctattcc
    15601 ttgcgcgcct tatgcgacct ttggaacacg cgaactttct gaacatgttg cgctggctct
    15661 caaaaatcgt aaggcaactt tgttacaaca tcatgggctt atcgcttgtg aggtgaatct
    15721 ggaaaaagcg ttatggctgg cgcatgaagt tgaagtgctg gcgcaacttt acctgacgac
    15781 cctggcgatt acggacccgg tgccagtgct gagcgatgaa gagattgccg tagtgctgga
    15841 gaaattcaaa acctatgggt tacggattga agagtaattt cgtaaagcaa caaggagaag
    15901 gatgatggct aacagaatga ttctgaacga aacggcatgg tttggtcggg gtgctgttgg
    15961 ggctttaacc gatgaggtga aacgccgtgg ttatcagaag gcgctgatcg tcaccgataa
    16021 aacgctggtg caatgcggcg tggtggcgaa agtgaccgat aagatggatg ctgcagggct
    16081 ggcatgggcg atttatgacg gcgtagtacc caacccaaca attactgtcg tcaaagaagg
    16141 gctcggtgta ttccagaata gcggcgcgga ttacctgatc gctattggtg gtggttctcc
    16201 acaggatact tgtaaagcga ttggcattat cagcaacaac ccggagtttg ccgatgtgcg
    16261 tagcctggaa ggactttccc cgaccaataa acccagtgta ccgattctgg caatccccac
    16321 cacagcaggc actgcggcag aagtgaccat taactacgtg atcactgacg aagaaaaacg
    16381 gcgcaagttt gtttgcgttg atccgcatga tatcccgcag gtggcgttta ttgacgctga
    16441 catgatggat ggtatgcctc cagcgctgaa agctgcgacg ggtgtcgatg cgctcactca
    16501 tgctattgag gggtatatta cccgtggcgc gtgggcgcta actgatgcgc tgcacattaa
    16561 agcgattgaa atcattgctg gggcgctgcg aggatcggtt gctggtgata aggatgccgg
    16621 agaagaaatg gcgctcgggc agtatgttgc gggtatgggc ttctcgaatg ttgggttagg
    16681 gttggtgcat ggtatggcgc atccactggg cgcgttttat aacactccac acggtgttgc
    16741 aaacgccatc ctactaccgc atgtcatgcg ctataacgct gactttaccg gtgagaagta
    16801 ccgcgatatc gcgcgcgtta tgggcgtgaa agtggaaggt atgagcctgg aagaggcgcg
    16861 taatgccgct gttgaagcgg tgtttgctct caaccgtgat gtcggtattc cgccacattt
    16921 gcgtgatgtt ggtgtacgca aggaagacat tccggcactg gctcaggcgg cactggatga
    16981 tgtttgtacc ggtggcaacc cgcgtgaagc aacgcttgag gatattgtag agctttacca
    17041 taccgcctgg taaatgcgct gatgtgataa tgccggatac gacgtttacg gcgtatccgg
    17101 ctcgccgtta ccgtaccaat cgcaattgct gtaaattgcc atcaatatgc aaatcggttt
    17161 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
    17221 gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga
    17281 ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc
    17341 tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
    17401 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
    17461 aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggtctgtg
    17521 tcactttgac cgccagcgtg gcggctaagt catcggcttc gttgccgctg gttgaccagc
    17581 acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt
    17641 ccggcattgg cggtcgaccc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac
    17701 tgcggttttc atcatcaaaa acggcgacag cgtgggttgg ctggctgtgc ataatgagct
    17761 gatcgagcgc gtgctggcag gtttcgacac agggcgaacc ctgaacggca tgaatgcgac
    17821 gaataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga
    17881 tcaggcgata agggtaacat tcgatactgg aacaggctat ggctggatga gaaatcggga
    17941 agaggcctcg caaaacgagg cctctggaga gcgattaatc gcaggcaacg atcttcattg
    18001 ccaggccgcc gcgagaggtt tcgcggtact tggcgttcat atctttacct gtttcgtaca
    18061 tggtttcgat aactttatcg aggcagacgc gcggctcgct ggtacggcgc agcgccatac
    18121 gtgcggcgtt caccgctttt actgccgcaa tggcgttacg ctcgatgcat ggcacctgga
    18181 cctgtccggc gaccgggtca cacgtcagac cgaggttgtg ctccatggcg atttccgccg
    18241 cgatgcacac ctgcgccggg cttgcgccta atagttctgc cagaccagcc gccgccattg
    18301 agcacgccac gccaacttca ccctggcaac ccacttcagc accagaaatc gacgcgttca
    18361 tcttataaag agaaccaatg gcgctggcta ccagcaggta acgagccagt gagttagcgt
    18421 tcacttcgcg gataaactta tcgtagtacg ccagcacagc cgggataatc ccgcacgcac
    18481 cgttagtcgg cgcagtcacc acgcgaccgc cagcggcgtt ctcttcgttc actgccagtg
    18541 caaacatgtt gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga
    18601 ccagcatccg gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggcaacacgc
    18661 cttcggtaga aataccgcgc tcaataccgc cgcgcatgac ttcccagacg ttcgccaggt
    18721 gctgttccag ctcttctttg ctgtgcagcg ccagctcgtt tttcatcatc aggccagaga
    18781 gtgacagccc ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag
    18841 gaacttcaac cggtgcgcta tcctgctggc caaaatgctc ttcatcaacg ataaagccac
    18901 cgccaataga gtagtaagtc tgactgtaaa cgactttatc acccgccagc gcggtaatgc
    18961 gcataccgtt ttcatgcaga gaaaggttgt ctgcatggaa gttcatgcac tgatcaaccg
    19021 ggaactccac ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct
    19081 gaataaaacc agggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca
    19141 taataatggc gatatcagtg tggtggcctt taccagtcag agagagcgag ccgtacacgt
    19201 caaccaccac gcgggtcacg tctttaagca ggttacgggc aatcagatcg tcggtaaatt
    19261 gtttacccgc tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga
    19321 aaatatcgaa tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc
    19381 gaaagcggcg cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca
    19441 gacccatcac gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta
    19501 ctttctgaat tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca
    19561 gggtttcaat catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca
    19621 gcatgaacag cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac
    19681 gcagagattt aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga
    19741 aggatttggt gatagcgata atcgcgataa tcggagccat ccacgcgata accggtgcgt
    19801 taaagtggtt agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt
    19861 ctgccggagt caggctcagt acgcagctga agacgaagaa cattacggtc agcaccatca
    19921 tgatgtgtgc gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt
    19981 cacgcttcgc aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta
    20041 ccggaattgc cagccacagg gtcatccaca gaccgtttcc ggttgcagat gcagtgtcca
    20101 gagacagcgt ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca
    20161 tcagtacgcc aacaaacggg aataccagaa tactcattgc tttaacgatc atctgctcac
    20221 cgaagcgaac gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg
    20281 gtggcgtcat acccagctgg tgagacatga agctttcaac tgtattggtg attgccacgc
    20341 tataaaccag caggatcggg tagatagcga agaagtagag cagggtaatc agtttacctg
    20401 cgccaatacc aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag
    20461 acagtacgaa gcgagtcagg ccgcggtgag caaaaaacgt catcgggaac gcaaggatag
    20521 ccatgatgat cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc
    20581 ccgcgccgat tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg
    20641 cactacgaga gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct
    20701 ggaggaatgc aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa
    20761 aaatgaaatc tgtctatcgg cgatggctta atcatttacc gccgtaataa tttgagggcg
    20821 gaaagataca cacaagttat gtatctatca gtgatcttga tcccaaatac aataatctac
    20881 ttggaagata gattatccca acgctttact ctgtaaatca aaagtaaaag agaatttaag
    20941 ggcaatattt gcccattaaa cgacatctga cttcttccgg ggcgcgcaat atatgta
//
LOCUS       DAZUGK010000070        20995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZUGK010000070 DAZUGK010000000
VERSION     DAZUGK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.070"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(255..569)
                     /gene="fliE"
                     /locus_tag="ACHY5W_002233"
     CDS             complement(255..569)
                     /gene="fliE"
                     /locus_tag="ACHY5W_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS4447126.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            784..2442
                     /gene="fliF"
                     /locus_tag="ACHY5W_002234"
     CDS             784..2442
                     /gene="fliF"
                     /locus_tag="ACHY5W_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS4447127.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSATVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVMAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2435..3430
                     /gene="fliG"
                     /locus_tag="ACHY5W_002235"
     CDS             2435..3430
                     /gene="fliG"
                     /locus_tag="ACHY5W_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS4447128.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3423..4109
                     /gene="fliH"
                     /locus_tag="ACHY5W_002236"
     CDS             3423..4109
                     /gene="fliH"
                     /locus_tag="ACHY5W_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS4447129.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4109..5449
                     /gene="fliI"
                     /locus_tag="ACHY5W_002237"
     CDS             4109..5449
                     /gene="fliI"
                     /locus_tag="ACHY5W_002237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS4447130.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASL"
     gene            5501..5944
                     /gene="fliJ"
                     /locus_tag="ACHY5W_002238"
     CDS             5501..5944
                     /gene="fliJ"
                     /locus_tag="ACHY5W_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS4447131.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5941..7068
                     /gene="fliK"
                     /locus_tag="ACHY5W_002239"
     CDS             5941..7068
                     /gene="fliK"
                     /locus_tag="ACHY5W_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS4447132.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KTAPQLLVATDKPMTKGEPLISDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSTQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7173..7637
                     /gene="fliL"
                     /locus_tag="ACHY5W_002240"
     CDS             7173..7637
                     /gene="fliL"
                     /locus_tag="ACHY5W_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFS4447133.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7642..8646
                     /gene="fliM"
                     /locus_tag="ACHY5W_002241"
     CDS             7642..8646
                     /gene="fliM"
                     /locus_tag="ACHY5W_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFS4447134.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8643..9056
                     /gene="fliN"
                     /locus_tag="ACHY5W_002242"
     CDS             8643..9056
                     /gene="fliN"
                     /locus_tag="ACHY5W_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFS4447135.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALGEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9059..9424
                     /gene="fliO"
                     /locus_tag="ACHY5W_002243"
     CDS             9059..9424
                     /gene="fliO"
                     /locus_tag="ACHY5W_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFS4447136.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRA"
     gene            9424..10161
                     /gene="fliP"
                     /locus_tag="ACHY5W_002244"
     CDS             9424..10161
                     /gene="fliP"
                     /locus_tag="ACHY5W_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS4447137.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10171..10440
                     /gene="fliQ"
                     /locus_tag="ACHY5W_002245"
     CDS             10171..10440
                     /gene="fliQ"
                     /locus_tag="ACHY5W_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFS4447138.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10448..11233
                     /gene="fliR"
                     /locus_tag="ACHY5W_002246"
     CDS             10448..11233
                     /gene="fliR"
                     /locus_tag="ACHY5W_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS4447139.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPTSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            11523..12146
                     /gene="rcsA"
                     /locus_tag="ACHY5W_002247"
     CDS             11523..12146
                     /gene="rcsA"
                     /locus_tag="ACHY5W_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFS4447140.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12190..12378)
                     /gene="dsrB"
                     /locus_tag="ACHY5W_002248"
     CDS             complement(12190..12378)
                     /gene="dsrB"
                     /locus_tag="ACHY5W_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFS4447141.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12541..12768
                     /gene="yodD"
                     /locus_tag="ACHY5W_002249"
     CDS             12541..12768
                     /gene="yodD"
                     /locus_tag="ACHY5W_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFS4447142.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13066..13881
                     /gene="yedP"
                     /locus_tag="ACHY5W_002250"
     CDS             13066..13881
                     /gene="yedP"
                     /locus_tag="ACHY5W_002250"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFS4447143.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKILGLQGLPLIAENGAVIQLAEQWQDIDGFPRINSGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13878..15572)
                     /gene="dgcQ"
                     /locus_tag="ACHY5W_002251"
     CDS             complement(13878..15572)
                     /gene="dgcQ"
                     /locus_tag="ACHY5W_002251"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFS4447144.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLIEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15743..15925)
                     /gene="yodC"
                     /locus_tag="ACHY5W_002252"
     CDS             complement(15743..15925)
                     /gene="yodC"
                     /locus_tag="ACHY5W_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFS4447145.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(16004..16921)
                     /gene="yedI"
                     /locus_tag="ACHY5W_002253"
     CDS             complement(16004..16921)
                     /gene="yedI"
                     /locus_tag="ACHY5W_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFS4447146.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLINQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSTLMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVEKMRGQAH"
     gene            17094..18014
                     /gene="yedA"
                     /locus_tag="ACHY5W_002254"
     CDS             17094..18014
                     /gene="yedA"
                     /locus_tag="ACHY5W_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFS4447147.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVDYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18003..18473)
                     /gene="vsr"
                     /locus_tag="ACHY5W_002255"
     CDS             complement(18003..18473)
                     /gene="vsr"
                     /locus_tag="ACHY5W_002255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFS4447148.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18454..19872)
                     /gene="dcm"
                     /locus_tag="ACHY5W_002256"
     CDS             complement(18454..19872)
                     /gene="dcm"
                     /locus_tag="ACHY5W_002256"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFS4447149.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPGQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(19939..20634)
                     /gene="yedJ"
                     /locus_tag="ACHY5W_002257"
     CDS             complement(19939..20634)
                     /gene="yedJ"
                     /locus_tag="ACHY5W_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFS4447150.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     YRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20674..20955)
                     /gene="drpB"
                     /locus_tag="ACHY5W_002258"
     CDS             complement(20674..20955)
                     /gene="drpB"
                     /locus_tag="ACHY5W_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFS4447151.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5060 a         5592 c         5432 g         4911 t
ORIGIN      
        1 aatatgaata aaaacatcaa caaagattaa attatccaga ggttactgtt gcgtctgtgg
       61 tcgtaacaac atccgacgtg gtgatgtgtc aaatgagacg ctctgatgtt caggggggtt
      121 atcgcgtata cggatcgtgg atggcagaaa acgttcagga tcaggtatcc attttaaacc
      181 agaaaatgag tgagtttgcc ccatccatgc cccacgcggt gagatcggat gtaattaaca
      241 acaggttaca aaacctacac ctgcatggac atcacttcct gatacgccgc caccagctta
      301 ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc ggtcatcaca
      361 tcgtttaacg ccacgccggg ttcaccgaga gtgaattttt ctgcctgcgt gcgggcggct
      421 gtttgtgtat cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc
      481 ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata
      541 accccttcaa tcccctgtat cgctgacatt ttcatctcct ggataatttc tggtagcaaa
      601 gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg
      661 gcggatagaa aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtttgctgac
      721 ccgggagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc
      781 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
      841 aatccgaaaa ttccattgat tgttgccggt tccgcgacag tggcggtcat ggtcgcactg
      901 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
      961 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc
     1021 agcggcgcta ttgaagttcc ggcagataaa gttcacgagc tgcgcctgcg cctggcgcaa
     1081 caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt
     1141 atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt
     1201 accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg
     1261 tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc
     1321 ggccgcgcac tcgatgaagg gcaaattagc gccattgtgc atctggtttc cagcgccgtt
     1381 gctggtctgc cgccgggaaa cgtcacgctg gtggatcagg gcggacatct gttaacccag
     1441 tccaatacca gcgggcgcga tcttaatgac gctcaattga aatatgccag cgatgtcgaa
     1501 ggccgtattc agcggcgtat tgaagcaatc ctgtcgccta ttgttggtaa cggtaatatt
     1561 cacgcccagg tcatggcgca actggacttc gccagtaaag aacaaacgga agaacagtat
     1621 cgccctaacg gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag
     1681 caaagcggtt ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct
     1741 gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg
     1801 agcaccacca gcaatagtgg gccgcgtagc acacaacgta atgaaaccag taactacgaa
     1861 gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc
     1921 gcggtagtgg tgaattacaa aaccttgcca gatggcaaac cgttgcctct cagcaacgaa
     1981 cagatgaagc aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt
     2041 gactcgctca atgtcgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagaactg
     2101 ccgttctggc aacagcaagc gtttatcgat cagttacttg ctgccggtcg ctggttgctg
     2161 gtactgctag tggcgtggct gctgtggcgg aaagcggtac gtccgcagct aacacgtcgc
     2221 gctgaggcga tgaaagctgt acagcaacag gcgcaggccc gcgaggaagt ggaagatgcg
     2281 gtggaagtcc gcctgagcaa agacgaacaa ctacaacaac ggcgcgctaa ccaacgtctg
     2341 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     2401 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     2461 aagcgtcatc ttgctgatga ccattggcga agaccgggcg gcagaagtgt tcaagcacct
     2521 ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     2581 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact
     2641 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaggaacg
     2701 tgccgccagc ctgctggaag atattctcga aactcgcgat accgccagcg gtattgaaac
     2761 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     2821 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     2881 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     2941 agccgcgctg gcggagctga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     3001 gcgcagcaaa atgggcggtg tgagaacggc agccgaaatt atcaacctga tgaaaactca
     3061 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgaactgg cgcagaaaat
     3121 catcgacgag atgttcctgt tcgaaaatct ggtggatgtc gacgatcgca gcatccagcg
     3181 tctgttgcag gaagtggatt ccgaatcgct gttgatcgcg ctgaaagggg ccgagcagcc
     3241 actgcgcgag aaattcctgc gcaatatgtc tcagcgtgcc gccgatattc tgcgcgacga
     3301 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct
     3361 gctgattgtg cgccgccttg ccgaaactgg cgagatggta attggcagcg gcgaggatac
     3421 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg
     3481 cggagtttgt gcccatggtc gagccggaag aaaccatcat tgaagaggcc gaacccagcc
     3541 ttgagcagca actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggga
     3601 ttgccgaagg tcgccagcaa ggtcataagc agggctatca ggaaggactg gcccaggggc
     3661 tggagcaagg tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc
     3721 aactggtcag cgaatttcaa actacccttg atgcacttga tagtgtgata gcgtcgcgcc
     3781 tgatgcagat ggcgctggag gcggcacgtc aggtcatcgg tcagacgcca acagtggata
     3841 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta
     3901 aaccacagct gcgcgtgcat ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcta
     3961 ccttaagttt gcatggctgg cggttgcggg gcgatcccac cctccatcct ggcggttgca
     4021 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     4081 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     4141 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     4201 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     4261 ctgtgtcatt gaacgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     4321 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg tcctgcccgg
     4381 cgcgcgtgta tatgccaaaa acatttcggc agaagggctg caaagcggca aacagttgcc
     4441 gctcggtccg gcgttattag gtcgcgttct ggacggcagc ggtaaaccgc tcgatggcct
     4501 cccctccccc gatacgactg aaaccggggc gctgattacc ccaccattta acccgttgca
     4561 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     4621 cgttgggcgt gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct
     4681 gctggggatg atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     4741 acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag ggcgtgcacg
     4801 ctccgtggtg attgccgctc cggcggatgt ctctccgctt ctgcgaatgc aaggtgccgc
     4861 ctatgccacg cgaattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     4921 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     4981 acccgccacc aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     5041 tgccggaaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgcttactga
     5101 aggcgatgac cagcaggacc ccattgcaga ctccgcgcgg gcgatcctcg acggtcacat
     5161 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     5221 gatcagccgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     5281 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     5341 caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta
     5401 tttacaacaa ggcatttttg aacgtgcgga ctgggaagcg tctctctagg ggctggagcg
     5461 tattttcccg acagtgtcat aacccaggag ataacggcag atggcagaac atggcgcgct
     5521 ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga
     5581 aatgcgtcgc gggtgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa
     5641 tgaatatcgc aataacctca acagcgatat gagtgccggg atgaccagca accgctggat
     5701 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     5761 taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg
     5821 tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     5881 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     5941 atgattcgct tagcgccctt gattaccgcc gacgttgaca ccaccacatt gcctggcggc
     6001 aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag
     6061 acaacaactg acaaaacggc cccccagttg ctggtggcaa cagataagcc catgacaaaa
     6121 ggcgagccgc tgatcagcga tattgtttcc gacgcgcaac aagcggattt actgatccct
     6181 gtggatgaaa caccgcctgt catcaacgac gaacaatcca catcaacacc gttaaccacc
     6241 gctcagacga tgacgttggc tgcggtggct gacaaaaata cgacaaaaga cgaaaaagcg
     6301 gatgatctga atgaagacgt caccgcaagc ctgagtgccc tttttgcgat gttgccgggt
     6361 tttgacaata cgcccaaagt gactgatgcg ccgtcaaccg tgttaccgac agagaaacca
     6421 acgctcttca caaaactgac ttctacgcaa ctcacaacag cacagcctga tgacgccccc
     6481 ggcacgccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     6541 atcagcacac cttcaccggt gaccgctgcc gccagcccgc taatcactcc acaccagaca
     6601 cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg
     6661 caacaatcat taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
     6721 ttgcgtctgc acccgcagga tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac
     6781 caggcgcaaa tccagatggt ttcaccgcat caacacgtac gcgccgccct ggaagcagcg
     6841 ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatc
     6901 agtggcgaaa gctttagtgg ccagcagcag gccgcttccc aacaacagca aagccaacgc
     6961 acagtaaacc atgaacctct ggcgggagaa gacgacgata cgcttccggt tcccgtctct
     7021 ttacaagggc gcgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag
     7081 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
     7141 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
     7201 gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg
     7261 caggttacag ctactggcat tcgcatcagg ttgccgctga agacaaagcg cagcaacgcg
     7321 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg
     7381 atcgcgtact ttatatcggc ataaccctgc gcctgaaaga tgaagctacc cgctcgcggc
     7441 tgagtgagta tttgccggaa gtccgtagtc gcttgctgtt actgttttcg cgtcaggatg
     7501 ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacac
     7561 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
     7621 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
     7681 ctgttgaatg gcgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt
     7741 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
     7801 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacttgctg
     7861 cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
     7921 cgcaacctgc cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca
     7981 gggctggtgg tgttctcgcc gagtctggtg tttatcgccg tggataacct gtttggcggc
     8041 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccataccga acagcgcgtc
     8101 atcaaccgca tgttgaaact ggcgcttgaa ggctatagcg acgcatggaa ggcgattaat
     8161 ccgctggaag ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc
     8221 tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
     8281 gaatttaata tctgcctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac
     8341 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
     8401 gtgcagcatt cacagctgga gctggtcgcc aactttgccg atatctcgct acgcctgtcg
     8461 cagattttaa aactgaaacc cggtgacgtc ctgccgatag aaaaacccga ccgcatcatc
     8521 gcccatgttg acggcgtccc ggtgctgacc agtcagtacg gcactctcaa cggtcagtat
     8581 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
     8641 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
     8701 ctgaggcgtt gggcgaacaa aaatcaacca gcagcaaaag cgctgccgac gcggtgttcc
     8761 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata
     8821 ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc
     8881 gtctgacgca agggtccgtc gtggcgctgg acggtctggc gggcgaacca ctggatattc
     8941 tgatcaacgg ttatttaatc gcccagggcg aagtggtggt cgttgccgat aaatatggcg
     9001 tgcggatcac cgatatcatt actccgtctg agcgaatgcg ccgcctgagc cgttagtgat
     9061 gaataaccac gctactgtgc aatcttccgc gccggtttct actgcgccac tgctgcaggt
     9121 gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
     9181 gttgggattt gctcctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
     9241 gctgggcgcg cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
     9301 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga
     9361 gataccgcag accgattttc agtcggtcat gaaaaatttg cttaagcgta gcgggagagc
     9421 ctgatgcgtc gtttattgtc tgtcgcacct gtccttctct ggctgattac gcccctcgcc
     9481 ttcgcgcaac tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg
     9541 ctcccggtgc aaacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg
     9601 atgatgacca gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcgctggga
     9661 acaccctccg cgccacctaa ccaggtattg ctggggctgg cactgttttt gacctttttt
     9721 attatgtcac cggtgatcga caaaatttat gttgatgcgt accagccatt cagcgaagag
     9781 aaaatatcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
     9841 cgtcagaccc gtgaggcaga tttagggttg tttgccagac tggcgaatac cggcccgttg
     9901 cagggacctg aagccgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg
     9961 aaaaccgcat ttcagatagg cttcacgatt ttcatccctt ttttgattat cgacctggtg
    10021 atagccagcg tgttgatggc attggggatg atgatggttc cccccgccac cattgctctg
    10081 ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg
    10141 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg
    10201 actgaagcga tgaaagtcgc gctggcactg gctgccccgc tattgttggt ggcgctggtc
    10261 acgggcctta tcatcagtat tttgcaggcc gccacgcaga ttaacgaaat gacgctatcg
    10321 tttattccga aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc
    10381 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    10441 ccgtactatg ttacaggtga caagcgaaca atggctatcc tggttaagcc tgtacttctg
    10501 gccgttactg cgcgtgctgg cgctcatttc taccgcacca attctgagcg aacgcagcgt
    10561 gccgaaacgg gtaaaactgg ggctggcaat gatgatcacg ttcgccattg ccccatcatt
    10621 acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg tgcagcagat
    10681 cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc
    10741 tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acgtttgtcg atccgaccag
    10801 ccatctcaat atgcccgttc tagcgcgtat catggatatg ctggcgttat tgctgttcct
    10861 gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc
    10921 gattggtggc gaaccgttga acagcaatgc gtttctggca ctcaccaaag cagggagttt
    10981 gattttcctt aacgggctga tgctggcgtt accgctcatt actctgctgc tgaccctgaa
    11041 tctggcatta ggtttactga atcgtatggc cccgcaatta tccatttttg ttattggatt
    11101 tccattaact ctgactgtcg gtatctcttt aatggcggca ttaatgccgt taattgcgcc
    11161 tttttgcgaa catttattca gtgagatgtt taatttgctg gctgatatta ttagtgaatt
    11221 gccattaata taattccata acgtttatca tgttatccta aggattatcc gaaaaataat
    11281 acctacgaac atcttccagg atactcctgc ggcgaaatat ttgttttaag ctcactcaca
    11341 tatcgcaaca tttactttac tttaagacaa ttccaggcaa attatacaac actttacggg
    11401 atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta ggtgacccat gttgttccgt
    11461 ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg
    11521 ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta accgggtatc
    11581 tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg
    11641 ccatagcttg tgattcacag cgcccttcag tggtgtttat taatgaggac tgtttcatcc
    11701 atgatgcttc taacagtcag cgcatcaagc acatcattaa tcaacatccc aatacgttat
    11761 ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa
    11821 atttattgat cagttctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata
    11881 ttctgaaaaa agagacaacg ataacctctt ttttaaatat gccgacgtta tcattgagcc
    11941 gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt caaatctctg
    12001 accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga
    12061 tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta
    12121 atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc
    12181 tgaataagat tactctgctt tctccacaaa gataccgtcc tgatgccctg cttcattaaa
    12241 gaaccagatg ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa
    12301 ctcctcaact gccagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac
    12361 ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg
    12421 tttttctggc agcgccaggc gcgcgcgagt gctgattttc tcgacggtct atacttaaga
    12481 gatgacaggc ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg
    12541 atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat
    12601 gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat
    12661 tctgaacacg ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc
    12721 ttcgaactgg atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa
    12781 aaaatcccga ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa
    12841 ttcgttacac caggaaatct gatgtgttca tcaccttatc cgcaattttt ttcgctgaca
    12901 agaaaatatt cgctatatga atgattaacg ctacttatga atagaaatgt gacgcacgtc
    12961 accccttggt ggctctggcg tcagcgggat cgctcccgaa aaaatcatct ttgattattt
    13021 gtgctgtttt gagaatcatc ctggtaaggg ggggactgcg cgaccatgct ttcaattcaa
    13081 caaccactac tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac
    13141 tggcaaccgg cagccccctg gctcagccgt ttacgcgaag caaatgttcc cgtcattctg
    13201 tgtagtagta aaacttcagc ggaaatgctg tacttgcaaa aaatattggg gctacaaggt
    13261 ttaccgctga ttgcagagaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata
    13321 gacggttttc cacgcatcaa ctcaggtatt agccatggcg aaatcagcca ggttttaaat
    13381 acgctacgcg agaaagaaca ttttaaattc acgacttttg atgatgtcga cgatgcaacc
    13441 atcgccgaat ggacgggatt aagccgtagc caggcggcgc tgacgcagct gcatgaggcg
    13501 tcggtaacgc taatctggcg cgacagtgac gagcgtatgg cacaatttac cgcgcgtctg
    13561 aacgaactgg gcttgcagtt tatgcagggt gcgcgcttct ggcacgtact ggatgcctct
    13621 gccggaaaag atcaggctgc caactggatt atcgcgacct atcaacaatt gtcaggcaaa
    13681 cgcccaacca cacttggcct gggcgatggg ccaaacgatg cgcccttact ggaggtaatg
    13741 gattacgcgg tgattgtgaa agggctaaac cgtgaagggg tgcatctgca tgatgaggat
    13801 ccggcccgcg tctggcgaac gcagcgtgaa ggaccggaag gatggcgtga agggctggac
    13861 cattttttct ccgcccgtta agcgttatcg ctcgcgaata cccgattacg cccagcctgt
    13921 ttagcgagat aaagccgacg gtcggccagt gactggagtt gttcaaaatc ataatcaccg
    13981 gtttcctcgc tgctacttac ccccaacgag gcactgatgc gtatcgtcgt actcttggcg
    14041 atcaacatct ctttttcaat taacttcagg cgaatacgtt ctgcgacctc cgcagcttcc
    14101 gtcagactcg cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg
    14161 tcctgcgcac gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc
    14221 gcctgatgac caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca
    14281 gaaaaaggat gttggtgcgt ctgacacaat ttcgcgagcg gacgggcttt ttcgaacagt
    14341 gcgccacggt tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt
    14401 tgcagaacat acatgttgct aaccatccgg cgaatcacat accaggagat gagtaacatg
    14461 gtggtaaaga gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc
    14521 acgccttcgc ttaacgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc
    14581 cagctaacat agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc
    14641 gccagcaagg ccagttcacg aggatcaaaa atattccctg ttggatggtc aggattggaa
    14701 gaggtcaaaa atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg
    14761 atggcgtttc ttaaaaattg ctgcatagta cgcacgggaa tactcatccc cagcacgcca
    14821 taccagtagt tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg
    14881 gcgtgttccg gttgcgaagt gaaccagcgt accgcgcggt gacgattttc tcgttgtgaa
    14941 tggccgataa accagggttg ggtgacatag ccgtaataac gcgtcgggac attgcgcgta
    15001 aacaaggtcg gctgcgtcga aacgtaaaat ccggcacgcg agacatacat cgcctgttca
    15061 accatcgacg aggagttgtg cgctagtcgc agcaagtaac caacttccag tgcagcggta
    15121 atttcattgt cgaggctttc attttcgcga gacaggagat tcccctcgct gactaatgca
    15181 tccgacacgc cattgactgg cagggtgcgc cgtcggttga gttcgatttt ccaggcgtgc
    15241 tcgtcgcgat gctgttcgaa ctcggttaca gcatcacgca gagaggtgaa atccagtggc
    15301 gctacgagag cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc
    15361 aaatgcttat cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag
    15421 gcatcttcca gcactaccac ttcacgccag gtcagcaagg tggaaaaaag caataccacg
    15481 ataaagcaga gattaacgat atgaccaggc cccaggcggc gtgcgagttt tttcaaccag
    15541 ctctggtttt ccatttttgt ctcgtgctgc accctgacga ctccccacct gcttttttat
    15601 gattctggca taacctggcg atagcgtacc gcaaaacagc gataatttta taatggtggc
    15661 atgataagac gcgactatca ttcattgcag gcattggcac atcaggccgg atgtgatccg
    15721 gcctggtctt aatgataccc tttcaaactt cttccgcagg acgactcccc tcccccggaa
    15781 caagctcggt ttcatgaaaa gcttcccgct tgaccccgta accgtcatac caacgacact
    15841 caaccatacc gctggagtat ccagtgacaa tcatccgcgg gccaccctct ttaaccgtaa
    15901 cttcctcact aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc
    15961 ttagttgaag atggcgaatt ttgcataata gcctgtgcga gttttaatgt gcctgaccgc
    16021 gcattttctc tacggctttc actcccagca ccacgatgcc gccgatgata aatccaagaa
    16081 tcagatttaa aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgaaat
    16141 gttcaatggc gtgatgcagc ggcgcaatac catgtaccac aatcccgccg ccgacgagga
    16201 acatcgccag cgtgccgaca atcgataacg ctttcatcag ccagggcgca atgatcaata
    16261 accctttacc taatgcctgc atcagcgtgc tggatttttc cgccagccaa taccccaggt
    16321 catcaatctt aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga
    16381 tgcctgacag taccagcacc tgattaatca acggcgcttc ggccacaatc cccagcgtga
    16441 tggcgacgat ttcggcagac aatataaaat cggtacgaat cgctcctttt attttgtcct
    16501 tttcaaactt cagcggatcc tgcgccgcca gcttctccag acgctgctgg ctctgcgccg
    16561 gatcttcctt atgtttacgc gcctccagca tatgcagcac tttctctacc ccttcaaagc
    16621 agagaaacgc gccaccaatc atcaacagag gcgtaatcgc ccacgggata aacgcactga
    16681 tgatcagcgc cagtggcacc agaatcactt tattaatcag cgatcctttc gccacgcccc
    16741 agaccacggg aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata
    16801 agtcatcccc taatacaccg gcggttttct tcgccgccag tttgcccatc acggagatat
    16861 cgtccagcag tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca
    16921 ttccctctca tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata
    16981 cttgcttcac aggtcaacat ttttttaaca atcaagtgca attatatctc tcgccagcag
    17041 aatgattttc acgtttacta tgaaccacct ttttatttcc accgtgaggg attatgcgtt
    17101 tccgccagtt attaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa
    17161 cctattttgt cattcggatt ggcgtggaaa gctggcctcc attaatgatg gcgggcgttc
    17221 gattcctggc agccggtatt ttattgctgg catttttgct actgcgcgga cacaagctcc
    17281 ccccgctacg tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta
    17341 atggcatggt gacggttgcc gaacatcaaa atgttccttc cggcatcgcc gccgtagtgg
    17401 ttgcaaccgt gcccctcttt accctgtgct tcagccgcct gtttggcatt aaaacgcgca
    17461 aactggaatg ggtgggtatc gccattgggc ttgccggaat catcatgctc aatagcggtg
    17521 gaaatttaag cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg
    17581 cgtttggctc agtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga
    17641 ttgagatgct ggcggcaggc gtggtgttaa tgatcgcgtc gatgattgcg ggtgaaaaac
    17701 tgacggcgct cccttccctt tcaggcttcc ttgcggtcga ctatctggcg ctgtttggtt
    17761 cgattatcgc catcaacgct tatatgtatt taatccgcaa cgtcagtccg gctctcgcca
    17821 ccagctacgc ttacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggcggag
    17881 agacgttgtc gaagattgaa tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg
    17941 tcacgttggg aaaatatctc tttccggcaa aacccgtagt tgcgccagtt attcaggacg
    18001 catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag
    18061 atccactctt ccagacgctc gctaagcgct tcatccgtca gcttctcgcg cccacgtaac
    18121 gcgcactccc agacaatcaa tacgcgccag ccgagttcct gcaagcgact gatatcgcgg
    18181 cgatcgcgct caacattttt acctatcttc tccagccaga actcggttcg agtcgcaggc
    18241 actttaaaca gatagcagtg atgatgatgc cagaagcagc catgggtaaa tatcacgcag
    18301 cgatattcat caacgacaaa atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac
    18361 gccaggccct gcccggttaa cagactggcg aggcgcttct caatcgccgt atcacgcgtg
    18421 gcaatcgcgc gcatattttt gctgcgagtg gccttatcgt gaacgtcggc catgttgtgc
    18481 ctcttgctga cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc
    18541 ggcaaagacc ggcacgacca ccgagttacc gaactggcga taggcctgag tgtccgaaac
    18601 cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcccg
    18661 aggcgttaac cgacgtggac gatgttgctg attctgcgga tcgtcaaagt ctttctcacc
    18721 cgtggccata tcccagccgc gatcgattaa aatttccgcg ccatctttgt aataacgcgc
    18781 agacagcgta cgggtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc
    18841 gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt
    18901 caggatatat ttcgcctcga ccatcgggtc cagcagctgc gctagcgtca ctcgctgtcc
    18961 agggaaacat tcgctgatat cacgcagagt aaaatcggct ttaagattca gatcgcgacg
    19021 aaaacccacc agcacgatgc gttcacggtg ctgcggcaga aaatgtttac catcgatgat
    19081 tttcggatcg tccggcccgt tatcttctgc atcagccacg tcatagccca gttcgtccag
    19141 cgtctgcatg atgatgcgga acgttttacc ctggtcgtgg cttttcaggt ttttgacgtt
    19201 ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg cgcaccacat caaagaacag
    19261 cgtgccctga gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac
    19321 gccagccagc gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat
    19381 gtgttgacga atatgttccg ccgccgcctc atcactcacg ccttctttat ggctgagggt
    19441 gatgtcgcgg atatcttcat taaaatgatg cgtcgccgga tcgcaataat ggttggcttt
    19501 ataagtgcgt accgcatgtt tgttccattc gctggtaaac acgcactgtc cgccaatcga
    19561 ttcaaaaccg cgacggatgc cgccaattcc ggcgaataga tcgataaagc gaaacgcata
    19621 atgcggatga tgtgccggtg gtttgggtaa caacgtttgc agatgggcga actctttctc
    19681 acttaaacgg tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca
    19741 gtgattctca cctacaccat taagctgagc caccagcgtt ttaacatcat aaatttgcag
    19801 cagtttctcc agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat
    19861 attttcctgc atagatttaa ccggccatct gagatggtcg acaggttaac ataattactg
    19921 ccgtttaggg ccacgcgctc agcccgcagg tgaaaacgca tctatcacct tgtgatcgac
    19981 accttcattc tcccccgcca gttcggcact gagcttcgcc ataaactcga ctaaaaattg
    20041 cgcgttgtgc tgcgccagct gcttgcctct tacggtttgc atggtttgcg gtagcttcag
    20101 caacttagtc tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc gatactgtgc
    20161 gaacggatct tcgccatcaa acagcgccac gcccaatgcc cctgaaacgg caaacacgcg
    20221 cgccaggcca atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc
    20281 cgtggttaag ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc
    20341 ctcgattttc tccgccggaa attgctcgaa ctcttcacgg agcaggcgac gcgtctcttc
    20401 tgccgccagg attgaagaac gctgccgttg cggatgattt ttcgccaagc tcacaatatc
    20461 gtgaaaataa caggcggtta aaatcaccag catatcgacg tcatcatctg ccgccagctt
    20521 ctgtgccgta gcccagacgc ggcgaaaatg acacacgtca tgggccgcgt cctggtgctg
    20581 atgatggttt ttcaaccagt tttcgaactc tgcctgccag tgttgtaagt ccatacgcgc
    20641 tccgttgcca aagagtcgcc accttagcag tttttattca tagcgtctgc tacgtgcggg
    20701 acgaggtcgg gtgtaccagg caatgctgcc taacaatgcc ccgaccagag ccataaataa
    20761 aaatccgacc agtgccccta accactttcc cgcggtagag cctaaatcgc tttcaaatcc
    20821 cgacacgggg gcatcgggga tccggctcat cacccagatg taacgcgcca ttgcccagac
    20881 gaaatagcca caccatgtat agaaagccca caaagccagt ttgccaccag ggctgcgaga
    20941 gagtctctct tccatctttg tcgaaccgta ttccattttg agataacccg gaaaa
//
LOCUS       DAZUGK010000071        20585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZUGK010000071 DAZUGK010000000
VERSION     DAZUGK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.071"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1512
                     /gene="mltF"
                     /locus_tag="ACHY5W_002259"
     CDS             <1..1512
                     /gene="mltF"
                     /locus_tag="ACHY5W_002259"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFS4447152.1"
                     /translation="LLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEI
                     NGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVK
                     NYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPEL
                     SWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFS
                     PLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQL
                     KPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDA
                     EQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDS
                     WADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEA
                     AMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKHN"
     gene            complement(1509..2012)
                     /gene="tadA"
                     /locus_tag="ACHY5W_002260"
     CDS             complement(1509..2012)
                     /gene="tadA"
                     /locus_tag="ACHY5W_002260"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4447153.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2070..2705)
                     /gene="yfhb"
                     /locus_tag="ACHY5W_002261"
     CDS             complement(2070..2705)
                     /gene="yfhb"
                     /locus_tag="ACHY5W_002261"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFS4447154.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTSRGELQQLE"
     gene            2914..3762
                     /gene="yfhH"
                     /locus_tag="ACHY5W_002262"
     CDS             2914..3762
                     /gene="yfhH"
                     /locus_tag="ACHY5W_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4447155.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3818..4078
                     /gene="yfhL"
                     /locus_tag="ACHY5W_002263"
     CDS             3818..4078
                     /gene="yfhL"
                     /locus_tag="ACHY5W_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFS4447156.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(4273..4353)
                     /gene="shoB"
                     /locus_tag="ACHY5W_002264"
     CDS             complement(4273..4353)
                     /gene="shoB"
                     /locus_tag="ACHY5W_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFS4447157.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(4774..5154)
                     /gene="acpS"
                     /locus_tag="ACHY5W_002265"
     CDS             complement(4774..5154)
                     /gene="acpS"
                     /locus_tag="ACHY5W_002265"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4447158.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHD
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(5154..5885)
                     /gene="pdxJ"
                     /locus_tag="ACHY5W_002266"
     CDS             complement(5154..5885)
                     /gene="pdxJ"
                     /locus_tag="ACHY5W_002266"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFS4447159.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(5897..6625)
                     /gene="recO"
                     /locus_tag="ACHY5W_002267"
     CDS             complement(5897..6625)
                     /gene="recO"
                     /locus_tag="ACHY5W_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4447160.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGGTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(6637..7542)
                     /gene="era"
                     /locus_tag="ACHY5W_002268"
     CDS             complement(6637..7542)
                     /gene="era"
                     /locus_tag="ACHY5W_002268"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4447161.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(7539..8219)
                     /gene="rnc"
                     /locus_tag="ACHY5W_002269"
     CDS             complement(7539..8219)
                     /gene="rnc"
                     /locus_tag="ACHY5W_002269"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4447162.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(8492..9466)
                     /gene="lepB"
                     /locus_tag="ACHY5W_002270"
     CDS             complement(8492..9466)
                     /gene="lepB"
                     /locus_tag="ACHY5W_002270"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4447163.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSRFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(9482..11281)
                     /gene="lepA"
                     /locus_tag="ACHY5W_002271"
     CDS             complement(9482..11281)
                     /gene="lepA"
                     /locus_tag="ACHY5W_002271"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4447164.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(11479..11958)
                     /gene="rseC"
                     /locus_tag="ACHY5W_002272"
     CDS             complement(11479..11958)
                     /gene="rseC"
                     /locus_tag="ACHY5W_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFS4447165.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFLLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(11955..12911)
                     /gene="rseB"
                     /locus_tag="ACHY5W_002273"
     CDS             complement(11955..12911)
                     /gene="rseB"
                     /locus_tag="ACHY5W_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFS4447166.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(12911..13561)
                     /gene="rseA"
                     /locus_tag="ACHY5W_002274"
     CDS             complement(12911..13561)
                     /gene="rseA"
                     /locus_tag="ACHY5W_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFS4447167.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(13594..14169)
                     /gene="rpoE"
                     /locus_tag="ACHY5W_002275"
     CDS             complement(13594..14169)
                     /gene="rpoE"
                     /locus_tag="ACHY5W_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFS4447168.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(14166..14321)
                     /gene="rseD"
                     /locus_tag="ACHY5W_002276"
     CDS             complement(14166..14321)
                     /gene="rseD"
                     /locus_tag="ACHY5W_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFS4447169.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            14577..16199
                     /gene="nadB"
                     /locus_tag="ACHY5W_002277"
     CDS             14577..16199
                     /gene="nadB"
                     /locus_tag="ACHY5W_002277"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFS4447170.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIKPNGEESYHLTREGGHSHRRILHAADATGKEVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(16184..16921)
                     /gene="trmN"
                     /locus_tag="ACHY5W_002278"
     CDS             complement(16184..16921)
                     /gene="trmN"
                     /locus_tag="ACHY5W_002278"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFS4447171.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            17053..18387
                     /gene="srmB"
                     /locus_tag="ACHY5W_002279"
     CDS             17053..18387
                     /gene="srmB"
                     /locus_tag="ACHY5W_002279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFS4447172.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(18420..19301)
                     /gene="yfiE"
                     /locus_tag="ACHY5W_002280"
     CDS             complement(18420..19301)
                     /gene="yfiE"
                     /locus_tag="ACHY5W_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4447173.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            19404..19991
                     /gene="eamB"
                     /locus_tag="ACHY5W_002281"
     CDS             19404..19991
                     /gene="eamB"
                     /locus_tag="ACHY5W_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFS4447174.1"
                     /translation="MTPTLFSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(20047..20430)
                     /gene="grcA"
                     /locus_tag="ACHY5W_002282"
     CDS             complement(20047..20430)
                     /gene="grcA"
                     /locus_tag="ACHY5W_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFS4447175.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT         5051 a         5439 c         5077 g         5018 t
ORIGIN      
        1 ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc cgacaaccgt
       61 atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac tcccctgact
      121 tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa acagtttgcc
      181 gattacctcg gtgtaaaact gaaagtgacc gtgcggcaga atatcagcca gctgtttgac
      241 gacctcgata atggtaacgc cgacctgctg gcggcaggac ttgtctataa cagtgagcgg
      301 gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact ggtttataaa
      361 gtgggtcagt atcgcccacg tacgctgggc aacctgacgg cggagcaact taccgttgca
      421 ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt cccggaatta
      481 agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt catcgaagga
      541 aaactcgatt acaccattgc tgattctgtc gccatcagcc tgtttcagcg cgttcacccg
      601 gagctcgccg tagcgctcga tatcaccgat gaacaaccgg tgacctggtt tagcccgtta
      661 gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat gaatgaagac
      721 ggtacgctgg cacgcattga agagaaatac ctggggcatg gcgatgattt tgattacgtc
      781 gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt
      841 gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta tcaggaatcg
      901 cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa
      961 aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag catcagcggt
     1021 ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa
     1081 cggatctggt ttgccctcgc tgcgtacaat atgggctatg cgcatatgct ggatgcccgc
     1141 gccctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa acagcgtctg
     1201 cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc tcgtgggcat
     1261 gaagcctacg cctatgtcga aaatattcgt aagtatcaga ttagcctggt gggttatctg
     1321 caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga ttatccggcg
     1381 gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct ttcccagtcg
     1441 tcatcaaact atttgaccca ttctccctct ctgctgtttt ccaggaaagg gagtgaagag
     1501 aaacataatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc ctggcggcgc
     1561 atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc ttccgtaatt
     1621 tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc cgcagcgcca
     1681 gttttcgcgt cacgcgcgcc aaagaccacg cgaccaatgc gactgtggat catcgctccg
     1741 gcacacatta cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag acgataattt
     1801 tgcatcacca gaccgccctg cctcagggcc atgatttctg catgtgcggt gggatcatgt
     1861 cgaccaatcg gacggttcca gccttcgcca ataacacgat tgttatgcac taataccgcg
     1921 ccgaccggta cttcccgctc atcccaggca cgcttcgcca gcgtcattgc atgacgcatc
     1981 cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg
     2041 cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc accgcgcgag
     2101 gttactcgcc aacgatgctg acagaaataa agcagcggat tgtcctgatt actgtcgcta
     2161 tagccactgt acagccgcag cggcgtgccg attttgcgct ccagttgtgc gaccttttca
     2221 tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat ttggctggcg
     2281 ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc
     2341 ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt ggttaatcgc
     2401 tcctgaacca gcggaaaggc ggtaacattg tcacgaaacc agcgtacgaa atcggcctgc
     2461 aacgcctgca aacgcgcttc attgtgacca aaagtgcatc cccacagaag cagactcatc
     2521 ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg caacaacggc
     2581 aggacaagta acgcattcag cggttggcga cgcagtaaat agcgcagaaa actgccgaac
     2641 atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt
     2701 gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact acatagccta
     2761 acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac tggaattttt
     2821 aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtgtc tcatattgcc
     2881 tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag
     2941 cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct gctacaacct
     3001 gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt cagtcagtcc
     3061 agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct taagttggcg
     3121 ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca taaccaaatc
     3181 cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa tactgccgct
     3241 atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt aacaatgttg
     3301 cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt ggcgcaaaac
     3361 tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga tatgcatgcg
     3421 ctactcgcta ccgttcaggc gtcgtcccct gacgatctgt tattagccat ttcttacacc
     3481 ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg cggaaaagtg
     3541 ctggcgatta ccggctttac tcccaatgcc ctgcaacagc gcgcttctca ttgcctgtat
     3601 accattgccg aagaacaagc gacaaacagt gcttcaatct ctgcttgtca cgcgcaggga
     3661 atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact ggcaccagaa
     3721 cgtattcgtc atagtgaagc gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata
     3781 atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgctgttaa tcactaaaaa
     3841 atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt caatgggaga
     3901 tcatatctat gagattaaca gcgataagtg taccgaatgc gtagggcact acgagacacc
     3961 aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaagatccgc aacatgtcga
     4021 gacagaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata aaatttaata
     4081 ttctgctcct gaagtagagt attaattatt ttatagggaa accttaacgc tattatattt
     4141 atttaattga tgattattag cacaaacatt cactaagtta atttatatag tatctgccca
     4201 gacacttatt tatagttatt aaaggtgcgt ccgactggtt caccggacgc accttaagta
     4261 cgtttccttg tgttataaga acagaaggat cagctgtaaa acagcaatga tgattttgat
     4321 ggcccgttta ataaggtatc ggcaatcagt cattcgtttt tccttaaaca aggaatgcag
     4381 ccgttcagat tagcccttac atctccccaa aactgaacgt gcgagttatt gagggtgcat
     4441 gctgcactcc acaccagagc tttgacgaca ccactcgttt caatggggga attctgtggc
     4501 atggtgtaaa gcacagcaaa atcgccaata acgaaaccaa ttttaatgta cttacgaatt
     4561 ggcggtcacg ttaatcttgc catcaatatc gctttcttcg taaaggctcg agtttttatg
     4621 ataaagattg caagttgctt gtaaaagata actaccctga tccataatcg ctgttgttga
     4681 gggtgcgtgc tgcactaaat taaagttaca aagtaaaacc cccgttcctt accagttcgg
     4741 gggttttact ttttaaagag aacggtatta tttttaactt tcaataatta ccgtggcgca
     4801 agcatagtgg cgctcatcgg ccagcgtcac atgcatattt gcaacgccca gcttttccgc
     4861 cagttttaat gcctcgcccc acagtcgcag ccgtggctta ccaagctcat cgttaaatac
     4921 ttcaaactga ttaaacgcca gaccattgcg gatcccggtg ccaaacgctt ttgctgcggc
     4981 ttcttttacg gcaaagcgtt tcgccagaaa acgcaccggc tggtcgtgcg ttttccaaat
     5041 ttcccattcg ttatcgctta atacgcggcg tgccaggcgc tcaccggatc gggcgatcac
     5101 tgcttctatg cgagcgatct cgacgatatc cgtgcctaaa cctaatattg ccattagcca
     5161 cgcgcttcca gcatcaggcg cttcatttct gccaccgcat ctttcagtcc ggtcatcact
     5221 gcacgaccaa taatggcatg accgatattc agttcgtgca tctcagggat ggcggcaatg
     5281 gcttttacgt tgtgataggt cagaccgtgt ccggcgttaa ctttcaaacc gagactagcg
     5341 gcaaaggtcg cggctttggc gatacgcgcc agctcttgcg cctgttcggc gtcagttttg
     5401 gcatcagcat agcaaccggt gtggatctcg ataaacggtg cgccaacctc tgccgcagct
     5461 ttgatctgct cttcatcggc gtcgataaac agagaaacct gaatcccggc atccgccaga
     5521 cgtttgcagg catcgcgcat tttgtcacgc tgccctgcaa cgtccaggcc gccttcggtt
     5581 gttacttcct gacgcttttc tggtaccagg cagcaaaaat gcggcttcgt ctcaaccgcg
     5641 atcgccagca tctcttcggt caccgccatc tccagattca tgcgggtatc cagcgtctga
     5701 cgcaggatgc gcacatcgcg gtcagtaatg tggcggcgat cttcacgtaa atgcacggta
     5761 atgccgtccg cccccgcctg ctcggcaata aacgcggcct gcaccggatc cgggtaagcg
     5821 gtaccgcgcg cgttgcgcag cgtagcgata tggtcaatgt tgacgcctaa cagtaattca
     5881 gccatgacaa tcctcatcat tcataatgtg ttttcaccgt tcgcttaggc ataaattgcc
     5941 ggaacagttc cctgctcttt aaaggtttac cgccaagata cggcttaagc gccatgcggg
     6001 taaagcgttt cgcggcgcgc agtgtgtctg cgtcaggaaa ttcccgtgcg tttaacgctt
     6061 ttaactgcct tccggtgaac gttttattat cgataacgac gcttgcgata aacccttttt
     6121 cttcgcgata acgatacgtc atggtgtcat ctaccggctc gccgctaccc gcacaatggg
     6181 taaaattgac gccataaccc agatgcccga gcagtgccag ttcaaagcgg cgcagcgcgg
     6241 gttctggcgt accagtgccc cctgcaagag actgaatgca gtgcaagtaa tcgaaaaaaa
     6301 gttcagagaa gcgcgtctcg tattccagta cgcgggagag aagttcgttg atgtacagac
     6361 cgctgtaaag cgtgataccg cttaatggca gcgccagcga gacggcttca gcactgcgca
     6421 gcgttttgac ttcgccacgc ccgccaaaac gtagcaagag aggggtgaaa ggctgtaatg
     6481 cacctttcag ggtagagcgt ttagagcgtg cgcctttggc aaccagacgc acgcgccccg
     6541 attcctccgt gaagacgtcc agcatcaggc tggtttcgct ccacgggcga ctatgcagga
     6601 caaatgcgcg ctgccagcct tccatcggag ttactcttaa agatcgtcaa cgtaaccgag
     6661 actgcgcagt gcgcgttcgt cgtcggccca accggatttc acttttaccc acagttcaag
     6721 gtggacaggc gcttcgaaca tttcctgcat gtctttacgc gcttcaatcc cgatggtttt
     6781 gatcttggcc cctttgttgc caatgaccat cttcttctgc ccttcacgct caacgagaat
     6841 caaaccgttg atgtcataac caccgcgttc gttagagacg aaacgttcga tctccacggt
     6901 cacggagtac ggcagttcag cgccgaggaa acgcatcagt ttttcgcgga tgatttcaga
     6961 cgccataaaa cgctgtgagc gatcggtgat gtaatcttcc gggaagtgat gagtcgcttc
     7021 aggtagatgc ttacgcacga ttgccgcaat agtgtcaaca ttcagcccgg tttcggcaga
     7081 gattggcacg atatcgagga agttcatctg gcttgccagg aactgcaggt gcggcagcag
     7141 atcggctttc tcctgcacgt tgtccacttt gttcaccgcg aggattaccg gcgctttgcc
     7201 ttcgcgcagt ttgttgagca ccatttcgtc gtccggcgtc cagcgggtgc cttcaacgac
     7261 aaaaatcacc agctcaacat cgccaataga gctgctcgcc gctttgttca tcaggcggtt
     7321 aatggcgcgt ttttcttcca tatgcaggcc cggtgtatcg acgtagatcg cctgatacgc
     7381 gccttcagta tggatcccca caatgcggtg acgagttgtc tgcgccttgc gggaggtgat
     7441 ggagattttc tgccccagca gtttgttcaa caatgtggat ttgccaacgt tcggacgtcc
     7501 gacgatggca ataaatccgc agtaactttt atcgatgctc attccagctc cagttttttc
     7561 aacgcctgtt cggcggcagc ctgctcagcc ttacgacggc ttgaacctgt gccaaccacc
     7621 ggttcactca ggccgctgac ctggcagtgg atagtaaatt cctgatcgtg cgcttcgcca
     7681 cgtacctgga caaccagata agtcggcagc ggcagatggc gaccctgcaa atattcttgc
     7741 aagcgcgttt tcggatcttt ttgtttatcg cctgggctaa tttcgtccag acgagtttga
     7801 taccagttga ggattaattt ctcgacggtt tgaatatcac tgtcgaggaa tacgccacca
     7861 attaatgctt cgacggtgtc ggcgagaatt gactcacgac gaaatccacc gcttttaagt
     7921 tcacctggcc ctaaacgtaa gcactcgcct aactcaaatt cgcgcgccag ttccgccagc
     7981 gtattgccac ggaccagcgt ggcgcgcatc cggctcatat cgccttcatc cacacgaggg
     8041 aaacggtgat aaagcgcatt ggcgataacg tagctcagaa tagagtcgcc taaaaattct
     8101 aaacgctcgt tatgtttgct gctggcacta cgatgagtta atgcctgctg caacagttcc
     8161 tgatgattaa aagtgtagcc cagcttccgt tgaagccgat taattacgat ggggttcatg
     8221 cgataccaat aaataaatgc gtcaacaatt cagcacacga aacagacctg atatacatgg
     8281 ctctgctaac tgcttcgctg cagtttcagt ttagcggtat atggggacca acgctgtttc
     8341 gtgtgccgtg gcaacctgga ggtgccaacc ttaaacttcg ggggaatatt ctatacacaa
     8401 cgacggggga tgtcgttagc cacgggagat ttatctcata aataattcac gttgtcgcca
     8461 taacggcgac aacgtgaacg aagatgagct attaatggat gccgccaata cgacttaagc
     8521 gcacacctgt cggccattcg ccttcttgct tatcgaagct catccagata gccgttgccc
     8581 gaccgaccag attcgcttca ggcacaaagc cccagtaacg gctgtccgcg ctgttgtcgc
     8641 ggttgtcgcc catcatgaag tattgtcccg gaggaacaat ccaggttgcc agttgttgcc
     8701 ctggctgctg gtaatacatc cccacctgat cctgcgcaat cggcactgtc agaatgcggt
     8761 gcgtcacatc acccagtgtc tctttacgct cggaaagacg aattccattt tctttggttt
     8821 cgtttttcgg cacttcaaag aatctgctgg tcgcttcccc accattacgg cgtgagaagg
     8881 tctgaacgaa atcgctcggt tccacgtttg agtaggtgac cggcagcgcg ttttcacacg
     8941 cctggccgga actgcatccc ggttgaatcg tcagctcttt tgagaccgga tcgtaagtga
     9001 ctttatcgcc cggtaaaccc accgcgcgct tgatgtaatc aagctttgga tcttccggat
     9061 atttaaagac cacgatatcg ccgcgtttcg gatgaccggt ttcgatcagc gttttctggt
     9121 agataggatc tttaatgcca taagcaaact tctctaccag aataaaatca ccgattaaca
     9181 gagtcggcat catcgaacct gacgggatct ggaacggttc ataaataaac gaacgcacaa
     9241 tcaatacgat agccagtacc ggaaaaacag aagcaccggt ttccagccag ccaggcttcg
     9301 gcgcaacctt tttcaacgtt gctttatcca gtgagtcacc ggcagccgcc tgcgccgctg
     9361 cctgacgttc ccgccgttta ggtgcgaaaa agaatttatc cacgcaccat aaaatgcccg
     9421 tcaccagtgt ggcaatcacc agaatcaggg caaacatatt cgccatgcca actcctaagg
     9481 gttatttgtt gtctttgccg acgtgcagaa tggcgaggaa cgcttcctgc ggcagctcga
     9541 cgttaccgat ctgcttcatg cgtttcttac cttctttctg cttctgcagc agctttttct
     9601 tacggctgat atcgccgcca taacatttag ccagtacgtt tttacgcagc tgtttcacgg
     9661 tggatcgcgc aataatgtgc gtaccaatcg ctgcctgaat ggcgatatca aactgctggc
     9721 gtgggatcag atctttcatc ttctccacca actcgcgacc gcggttttgc gaattatcac
     9781 ggtgggtgat caacgccagc gcatcaacac gttcaccgtt gattaatacg tctacacgta
     9841 ccatgtcgga cgcctggaag cgtttgaagt tgtaatccag agacgcataa ccacgcgagg
     9901 tagatttcag gcgatcgaag aagtcgagca ccacttccgc catcgggatc tcgtacgtca
     9961 gcgccacctg attaccgtgg taaaccatat tggtctgcac gccgcgtttt tctacgcaca
    10021 gcgtaataac gttgccgaga tatgcctgcg gcagcagcat gtgacactct gcaatcggct
    10081 cgcgcagttc gtaaatgtta tttaccgcag gcagcttgga tgggctgtcg acgtagataa
    10141 cttcccttga cgtggtttca acttcataca ctacggtcgg cgcagtggtg atcagatcca
    10201 gatcgtattc acgttccaga cgctcctgga tgatctccat gtgcagcagg ccaaggaagc
    10261 cgcagcggaa accaaagccc agcgcgctgg agctttccgg ctcatagaac agtgaggcat
    10321 cgttcaggct gagtttaccc agcgcgtcac ggaaggcttc atagtcgtcg gaacttaccg
    10381 ggaacagacc ggcgtatacc tgcggtttga ctttcttaaa gccaggcagc gccttttctg
    10441 ccggattacg cgccagcgtt aaggtatcgc cgactggagc gccgtggata tctttaatcg
    10501 cacatacgag ccagcctact tcgccacatt tcagttcagt gcggtcaacc tgtttcggcg
    10561 tgaagatgcc cagacggtcg gcgttatagg tctgcccggt actcatgact ttcactttat
    10621 cgcccttacg cagggtgccg tttttaatac ggataagtga aacaacaccc aggtagttgt
    10681 cgaaccagga gtcgataatt agcgcctgca acgggccttc cggatcgcct tccggcggcg
    10741 gaatgtcgcg caccagacgt tcgagaacgt cctgcacgcc aacgccggtt ttcgctgaac
    10801 agcgcaccgc gtcggtggcg tcgatgccga cgatatcttc aatttcttcc gccacgcgtt
    10861 caggatcggc tgccggcagg tcaatcttgt tcagtaccgg caccacttcg agatccattt
    10921 ccatggcggt gtagcagttt gccagggttt gcgcttccac gccctgcccg gcgtcgacca
    10981 ccagcaatgc accttcacag gcagccagcg aacgggaaac ttcataggag aagtctacgt
    11041 ggcccggggt gtcgataaag ttaagctgat aggtttcgcc gtcagacgct ttgtagtcca
    11101 gcgtcacgct ttgcgctttg atggtaatgc cacgctcacg ctcaagatcc atggaatcga
    11161 gaacctgcgc ctccatttca cggtcagaca ggccaccgca gatctggata atacggtcag
    11221 acagcgtcga tttaccgtgg tcaatgtgag ctatgatcga aaagttacgt atattcttca
    11281 taaagtatga ttattgtgcc ttaatgcccg gttaaccagg cttttagaag tcgctgttct
    11341 gagcttaacg tctgtattaa tagaaacgcc gcattctaca ctacaacatt gaggcgagga
    11401 aatgttcata ccgtatggat tgtggtatct ggaaacgtcc tcgcatttgt tatgcaaaat
    11461 gcaacaaagc cagtgaaatc actggctcgc gtcttccgaa gatgtttcaa atcgcacaag
    11521 gccaggcggc aatgccacgc ttaagatgat cggttgccat tctgcccggg cagcaaactt
    11581 acgcgagtag ccacgcgcaa tcaggaaccc gccaataccg ccgaggaccg caccacataa
    11641 tgctgcgacg tcagaagcaa agagtagcag aaatagcgaa gcgatgagga ataatcccac
    11701 cagcggcgac atataaacca gtaatgcgga gctaagcagg ctgccttcgg cgatccccaa
    11761 ttccactttt tgccccggca ctaacggctc atcacagggt acgacaatgg tatgcgtggt
    11821 ttgcgggcca agtttattca acacgcggct accgcaaccg gcgcgtgaag cgcagctgct
    11881 gcatgaggct ttaacatcac aactgaccag cgcctgcccg ttttgccagg agacgacggt
    11941 agcccactct ttgatcattg cgctgccccg aacttaatat tctcggcaat gcgtttcgcc
    12001 gtttgcggcg gcagttcacc gacaatggtg atttcggcgt tatcacggac gcttgtactg
    12061 acggttctgc gtccggtgcg caacatctga tcggtgctcg atggcgtagc gcggttaacg
    12121 tttaccgaga agctgaataa tccgtcggaa tagagacgtg attcgatagg catgttgtcc
    12181 atcgtcggta gcggacgtcg actactggaa acttcgctaa aaccctgtgg caaccaggtt
    12241 ggcgtccagc tgaatttagc tttttcacct acaggaacag aaagcaacgg cggcaaattt
    12301 gccttcgcca gcgtctgcat actgctgctg atatcctgat tgacgttaaa agcaatcacg
    12361 cgaaattgtt ccagcgtttc accatcgcga tcaagaagat caacccgcat cggtaatttc
    12421 gattcggtgt ccatccacac gatgtagctg tagcgtgtac catctcgggc aaccacgcga
    12481 atgacttcgc aaagacgatc agcaatacgc gtgcgcccga cggagataaa gtcgtagtaa
    12541 ggagaaaggc gtttgaaatc ggtatagatg agcgatggca gagaatcaac aatgtaatcg
    12601 ccattaagcg tgaacggttc aagtcccggt tcaaaatagc tgatttcatt gccgcgctgt
    12661 accacttccc ggcgcgggcc atccatttgc aacaattgtg caagaggacg gttatcgagg
    12721 cgtgcatgtc gataacgcag agactcaaca ccctgtttat tgatgctgat gaatgacagc
    12781 tcgtaattca gtgactgact ggccaggttc atctgctgta ataacgcccc ggacgcggga
    12841 gtggccgagg cgttagcaga gaataacagg ctacctgtca ctaatgacat ggcaaaccaa
    12901 agttgcttca ttactgcgat tgcgttccta aagtttgaat tcctggcacc tgtacagcgg
    12961 cttgctgggt ttgcgcctgc tcaaactgaa gctgttcaga gtggagtcgg cgttgcagtt
    13021 cgtaatcctg caacattgca ttaatgcgac gacgctgctc ctgtacctgc tgctgttgac
    13081 cattatttgc ggtcgcttca gaaggtactc ccaggctaac cgggctggct ttacccatca
    13141 tcggcagtgt attaaatacc ggcgtttcgg gctgctggga cgtttcagat tgtccattat
    13201 agtgctggac gccaacgata actgcaagcg atacgcatgc ggctacgccc atttgggtaa
    13261 gctgtgccgc ccacggacgt actttctgcc agaatggcat tttctgccat tgatgcggcg
    13321 caggctgggc ttccgggatc aatgtcgccg gttgacgtac tggctcttct tcaatggcgg
    13381 ccatcacgcg tgaagagata tcgaaatgga gcacctcggg agtatcaccc cgcattgagt
    13441 cacggattaa gtgatagctt tcccaggttt tctgcatttc tgggttatga gccagttcgt
    13501 taagcagctc actatccagc gtttcgccat ccattaaagc ggaaagttgt tctttctgca
    13561 tgcctaatac ccttatccag tatcccgcta tcgtcaacgc ctgataagcg gttgaacttt
    13621 gttatcaata gcttccctcg ctcggaagat acgtgaacgc accgtaccta ccggacaatc
    13681 catgatagcg gctatctctt catagctcag gccatccagc tcccgcaagg ttattgccat
    13741 gcgtaaatct tccgggaggg actcaatagt tcggaaaact atctgtctca gttcttctga
    13801 caacattaag ttctcagggt tcgaaatttc tttcaacgcg ccgccacttt cgaagttttc
    13861 agcttcaatg gcatccacat cactggaagg tggacgacgc ccctgagcaa ccaggtaatt
    13921 tttcgctgta tttacagcaa tccgatacag ccatgtataa aaagcactat ctccccggaa
    13981 cgaatccagc gcacgatagg ctttaataaa agcttcttgt accacatcgg gaacatcacc
    14041 cgacggcaca tagcgggaaa ccagactcgc cactttatgc tgatagcgca ctaccagtaa
    14101 gttaaaggct ttctgatctc ccttctggac ccgttcaacc aggacctggt ccgttaactg
    14161 ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc acgcgctatc gaaacgccac
    14221 tccattagct gcaatttgag caagcaaagg gttagagtgt ctcgtttttg taaagttccg
    14281 taacgcatct gtttttgttt gtcatgctgt agacggatca ttatctatca ttataagtct
    14341 acagaatctg aacatcgcat tatctgtgta gaaatgccca tttaactgcc tgaagagtaa
    14401 cccaacggcc tttttatttc accacctaat cctccaccag ccagtaactt ctctttttct
    14461 cgccgccctg cgtcagcgtg tttagccact gtaacaaata ttaaaatagc aggtgtttat
    14521 tcgcacaaca tgatgctatg ctgaccaaac catgtttagt aaattaaaca aagaaaatga
    14581 atactctccc agaacattca tgtgacgtgt tgattatcgg tagcggcgca gccggacttt
    14641 cactggcgct acgcctggct gaccagcatc aggtcatcgt tctaagtaaa ggcccggtaa
    14701 cggaaggttc aacattttat gcccagggcg gtattgccgc cgtgtttgat gaaactgaca
    14761 gcattgactc gcatgtggaa gacacattga ttgccggggc tggtatttgc gatcgccatg
    14821 cagttgaatt tgtcgccagc aatgcacgat cctgtgtgca atggctaatc gaccaggggg
    14881 tgttgtttga tacccacatt aagccgaatg gcgaagaaag ttaccatctg acccgtgaag
    14941 gtggacatag tcaccgtcgt attcttcatg ccgccgatgc caccggtaaa gaagtagaaa
    15001 ccacgctggt gagcaaggcg ctgaaccatc cgaatattcg tgtgctggag cgcagcaacg
    15061 cggttgatct gattgtttct gacaaaattg gcctgccggg cacgcgacgg gttgttggcg
    15121 cgtgggtatg gaaccgtaat aaagaaacgg tggaaacctg ccacgcaaaa gcggtggtgc
    15181 tggcaactgg cggtgcgtcg aaggtttatc agtacaccac caatccggat atttcttctg
    15241 gcgatggcat tgctatggcg tggcgcgcag gctgccgggt tgccaatctc gaatttaatc
    15301 agttccaccc taccgcgctg tatcacccac aggcacgcaa tttcctgtta acagaagcac
    15361 tgcgcggcga aggcgcttat ctcaagcgcc cggatggtac ccgttttatg cccgattttg
    15421 atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac catgaaatga
    15481 aacgcctcgg cgcagattgt atgttccttg atatcagcca taagcccgcc gattttattc
    15541 gccagcattt cccgatgatt tatgaaaagc tgctcgggct ggggattgat ctcacacaag
    15601 aaccggtacc gattgtgcct gctgcacatt atacctgcgg tggtgtaatg gttgatgatc
    15661 atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat accggcttac
    15721 acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat ggctggtcgg
    15781 cggcggaaga tatcaccaga cgtatgcctt atgcccacga catcagtacg ttaccgccgt
    15841 gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat aactggcacg
    15901 agctacgtct gtttatgtgg gattacgttg gcattgtgcg cacaacgaag cgcctggaac
    15961 gcgccctgcg gcggataacc atgctccaac aagaaataga cgaatattac gcccatttcc
    16021 gcgtctcaaa taatttgctg gagctgcgta atctggtaca ggttgccgag ttgattgttc
    16081 gctgtgcaat gatgcgtaaa gagagtcggg ggttgcattt cacgctggat tatccggaac
    16141 tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata aacagataaa
    16201 aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca cgaatgacta
    16261 agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc cgatgcggca
    16321 gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc atacttaatg
    16381 ccagttcagt aaaaccatta ccaatctgct ctggcagcac cacgcagaaa aatccctctt
    16441 cggtaatgca ctccgccgca caggtcagca acgatgggtg atcaagcgta gtggtatagc
    16501 gagcctgttc ccgttgaggt gtcgagcact ctactccctg ctgatagtaa ggtgggttac
    16561 tgatgattaa atcgaagcgt actgtctgct gtgtgatcca ctgctgaata tccgccgtat
    16621 ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcttgcgcc tgcgcggcag
    16681 cttcactttc cagttcaact gcatcaatca tcacgctgtc atcggttcgc tgcgccagca
    16741 ttaatgccag caacccgcta cccgcgccga tatcaaggca acgttttacc ccagccaccg
    16801 gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacaa cgatcgtgag
    16861 caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta gactgtgaca
    16921 tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa tacccgagaa
    16981 gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca gcgccccaca
    17041 cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga aagcctgctg
    17101 gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc cgccattccg
    17161 cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg taaaacggcg
    17221 gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cacgtaagaa atccggtccg
    17281 ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc cgatcatgcc
    17341 cgcgaactgg cgaaacatac gcatctggat atcgccacca tcaccggcgg cgtagcctat
    17401 atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac gaccggacgt
    17461 ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac gctgatcctc
    17521 gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca tattgctggc
    17581 gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa ctctggaagg cgatgcgatt
    17641 caggactttg ccgagcgtct gctggaagat ccggtggaag tttctgccaa tccgtccacc
    17701 cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgatcttga gcataaaacc
    17761 gcgttgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt gtttgtgcgt
    17821 aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat caacaactgc
    17881 tatctcgaag gtgagatggt acaaggcaag cgtaacgaag cgatcaagcg tttgaccgaa
    17941 ggtcgcgtaa acgtactggt tgcaaccgat gttgctgcgc gcggtatcga cattcctgac
    18001 gtcagccacg tctttaactt cgatatgccg cgcagtggcg atacttattt gcaccgtatc
    18061 ggacgtaccg cgcgcgccgg tcgtaaaggc accgcaattt cgctggtgga agcccatgac
    18121 catctgctgc tgggcaaagt tggtcgctat attgaagagc caattaaagc tcgcgtaatt
    18181 gatgagttac gcccgaaaac gcgtgcgcca agcgagaagc agaccggcaa gccatcgaag
    18241 aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa gccgcgggtg
    18301 aaaaaacgcc atcgcgatac caaaaatatt ggtaagcgcc gtaaaccaag cggaacgggc
    18361 gtgccaccgc aaacgacaga agagtaatct caatgccggg tttaagcccg gcattatttt
    18421 tatcccggca aaaaacaatc ttcaacacac tgaataaaag tgtgcatcgc cgggctaacc
    18481 gcttttccgg catggtgagc gcacattgcc gtaatggtct gcgagtgttc gccaaaaggc
    18541 aattcaatta attctccgct ctcaagctct ttttccaccg caaagcgggg caagtaactg
    18601 acaccgatat tcgccgcgac acaacgtttg atactttcaa tgcttaacag ctcaatggta
    18661 ttttccaccg tgatccgccg ctggcgcagc gtgctctcaa atatctgccg gaatacacat
    18721 tgcggttcgt tgataataaa gctacaggcg ttatgtcttc ccggctcagt aaaatcgaca
    18781 tctgcaattt gcggtgaagc taccagcacc agcgattgct cacccaattc tcgtcggttc
    18841 agggcatcat cattccctac gcgataaaaa accccgacat cggcctcatc attcaatagc
    18901 gcatcacgga tcacgtaaca gttcagagac tgcaacgata aacgcacttt tggggcgcgt
    18961 tgccgaaaac gctgcaacac ctgcggcatt cggtaagaga gcaacgtttc gcccgaaaca
    19021 acgcgtagtt ccccgtccgg atccgactcc tttttggcag cctcacgaag ggtatccatc
    19081 actctggtaa gttcatagat gtgcggcagc agctttttcc cttcgcgggt aaggcacatt
    19141 cgtcgaccaa ttttctcaaa taactgtact gagaactcct gctcaagctg ctgaatatga
    19201 aaagtcaccg tcgattgtgt gcagcacaat ttttgcgaag ctcgcaaaaa ggaaccctct
    19261 tccaccacgg ttttaagcgt aataaaacgg cgcagatcca taaccccaaa cctatcgaaa
    19321 atatcgaatt tagaatgtaa aaacattcat ttttttaaat gttccgtgtc gggtactgtc
    19381 taccaaaaca gaggagataa caagtgacac cgaccctttt cagtgctttt tggacttaca
    19441 ccctgattac cgccatgacg ccaggaccga acaatattct cgcccttagc tcagccacaa
    19501 cgcacggatt tcgtcagagt acccgcgtgc tggcagggat gagtctggga tttttaatcg
    19561 tcatgttgct gtgtgcgggc atttcgtttt cactggctgt tatcgacccg gcagctgtgc
    19621 atctgttgag ttgggcgggg gcggcatata ttgtctggct ggcgtggaaa atcgctacca
    19681 gtccaacaaa ggaagacggg cttcagacaa aaccaatcag tttttgggcc agctttgctt
    19741 tgcagtttgt gaacgtcaaa atcattttgt acggtgttac ggcactgtcg acatttgttc
    19801 tgccgcaaac acaggcgtta agctgggtag ttggcgtcag cgttttgctg gcgatgattg
    19861 ggacgtttgg caatgtgtgc tgggcgctgg cggggcatct gtttcagcga ttgtttcgcc
    19921 agtatggtcg ccagttgaat atcgtgcttg cactgttgct ggtctattgc gcggtacgca
    19981 ttttctatta acgaaaaaaa gcggaagaag tcgccctctt ccgcttagta acttgctact
    20041 taagccttac aggctttcag taaaggtacg agcaataacg tcgcgctgct gttccggagt
    20101 cagagagtta aagcgaactg catatccgga tacacggatg gtcagctgcg gatatttttc
    20161 cggatgctta actgcatctt ccagagtttc acgacgcaga acgttaacgt tcaggtgctg
    20221 accaccttca acgcgaactt ctggtttcac ttctactgga acttcacggt actcaatatc
    20281 acccagtttg cttactgcaa ccacttcgtc ttctgcaaaa cctgctttcg caacgatgca
    20341 acgcgcttcg cctttttcgc tgtccagcag ccagaaagag ttcagcagat cgtcgttagc
    20401 ggctttagta atctggatac ctgtaatcat gtgatgcctc cccggcaaaa ttatttgatt
    20461 tgttcagcct gtcgcggcca attggtaaaa ccattgttgc ttgagtgtat atatactcct
    20521 caaacaccct tgaatctttg atttaaatca ataaaaacca cacatcaagt atggtcgcaa
    20581 atgga
//
LOCUS       DAZUGK010000072        20280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZUGK010000072 DAZUGK010000000
VERSION     DAZUGK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.072"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHY5W_002283"
                     /gene_synonym="yiiD"
     CDS             complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHY5W_002283"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFS4447176.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHY5W_002284"
     CDS             complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHY5W_002284"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4447177.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHY5W_002285"
     CDS             complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHY5W_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFS4447178.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHY5W_002286"
     CDS             complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHY5W_002286"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFS4447179.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            3215..3493
                     /locus_tag="ACHY5W_002287"
                     /pseudo
     CDS             3215..3493
                     /locus_tag="ACHY5W_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            3484..3606
                     /locus_tag="ACHY5W_002288"
                     /pseudo
     CDS             3484..3606
                     /locus_tag="ACHY5W_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(3687..4952)
                     /gene="yihN"
                     /locus_tag="ACHY5W_002289"
     CDS             complement(3687..4952)
                     /gene="yihN"
                     /locus_tag="ACHY5W_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4447180.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(5054..6034)
                     /gene="yihM"
                     /locus_tag="ACHY5W_002290"
     CDS             complement(5054..6034)
                     /gene="yihM"
                     /locus_tag="ACHY5W_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS4447181.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(6042..6752)
                     /gene="yihL"
                     /locus_tag="ACHY5W_002291"
     CDS             complement(6042..6752)
                     /gene="yihL"
                     /locus_tag="ACHY5W_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4447182.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(6969..8792)
                     /gene="typA"
                     /locus_tag="ACHY5W_002292"
     CDS             complement(6969..8792)
                     /gene="typA"
                     /locus_tag="ACHY5W_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFS4447183.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            9165..10574
                     /gene="glnA"
                     /locus_tag="ACHY5W_002293"
     CDS             9165..10574
                     /gene="glnA"
                     /locus_tag="ACHY5W_002293"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFS4447184.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            10748..11797
                     /gene="glnL"
                     /locus_tag="ACHY5W_002294"
     CDS             10748..11797
                     /gene="glnL"
                     /locus_tag="ACHY5W_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFS4447185.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            11809..13218
                     /gene="glnG"
                     /locus_tag="ACHY5W_002295"
     CDS             11809..13218
                     /gene="glnG"
                     /locus_tag="ACHY5W_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFS4447186.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VDAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            13330..13440
                     /gene="yshB"
                     /locus_tag="ACHY5W_002296"
     CDS             13330..13440
                     /gene="yshB"
                     /locus_tag="ACHY5W_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFS4447187.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(13630..15003)
                     /gene="hemN"
                     /locus_tag="ACHY5W_002297"
     CDS             complement(13630..15003)
                     /gene="hemN"
                     /locus_tag="ACHY5W_002297"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFS4447188.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(15192..15698)
                     /gene="yihI"
                     /locus_tag="ACHY5W_002298"
     CDS             complement(15192..15698)
                     /gene="yihI"
                     /locus_tag="ACHY5W_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFS4447189.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(15817..15983)
                     /locus_tag="ACHY5W_002299"
                     /pseudo
     CDS             complement(15817..15983)
                     /locus_tag="ACHY5W_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16280..16912
                     /gene="yihA"
                     /locus_tag="ACHY5W_002300"
     CDS             16280..16912
                     /gene="yihA"
                     /locus_tag="ACHY5W_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4447190.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(17294..20080)
                     /gene="polA"
                     /locus_tag="ACHY5W_002301"
     CDS             complement(17294..20080)
                     /gene="polA"
                     /locus_tag="ACHY5W_002301"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4447191.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         4923 a         5256 c         4997 g         5104 t
ORIGIN      
        1 tgctcctgaa cagtgatgta gcaagacacg tatggagcca acgaagaata aaaatttata
       61 gagttcttat tcaatataac cgaaatagcc aaggactaat ataaatgacc tgaatgagag
      121 aggcagcacc gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc
      181 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
      241 cgccaaagat ttcgacctgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
      301 gatcgccgct taaggcacca aggtcggcta ccgcatgggg tttaccgcta atcggtttgc
      361 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcacgcagca
      421 tcagccagat aagcccccaa ccggtgagcg tcgccagtga gaataaactc ccggcaaaca
      481 gcgtatggtg cggattctga ttgccggttt ctggcatggt ggtgataaat ttttgcccgg
      541 tatattgttg aatgcgcacg cccatttttt cgcttaacgg gatatgttcg taccacgcct
      601 gttgcagctg cgcgcaccag tcaccgcgat gcagaatgtc atccagagtg gcaacgggtt
      661 taatcatcaa aaaatggcga atcggcgtgg tagttggggt ggtgatctcc ccctgattaa
      721 tgaatcccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
      781 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtg cctaaccctt
      841 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
      901 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
      961 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     1021 tttcccagcg aaactgatag taacgctcta actcttcttc tgtttgtgga acccgaaggt
     1081 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     1141 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg
     1201 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     1261 ctctgcgcga tccggtgatg cacctttgga gaaacttggg cgcatccccc gttcggtatc
     1321 tgcggcgagg gtaaactggg aaaccaccag cacactgccg cccgcctgtt gcacgttgag
     1381 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     1441 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     1501 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     1561 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     1621 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     1681 acaaagagaa tggggatcac cgccagcaca ccgtaaatga gctgatatga cgggaacatg
     1741 gtgatataaa gcgcgaaacc cttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     1801 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     1861 aaggagatcc acgacaacag cagcggaaaa atacgcagca cattgtcgat gacagtattg
     1921 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     1981 agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc
     2041 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     2101 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     2161 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     2221 tgacgcaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     2281 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     2341 gtcatgttgt cctcatcaat gcgttgccag agtagtttca gccaggccca tagtggacgg
     2401 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     2461 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgatta gctccttcta
     2521 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     2581 aaacatgctg gtaaattcga gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     2641 tatggtcagc agcatcacga atttccgggt attcctccgg ccagaaggta gtatgcaggc
     2701 ggttagtatt ggaaagcaca accacgcgat gcccctgctc acgcagttta tgcatgatgg
     2761 cgatcacttc cggtcgtagc gcaacaaata ccgcctgcca accgtgggag aactgctcat
     2821 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     2881 ccccacgctc atgctgatga aacgcctccc ccatatgaaa actcttctta agcgtagcca
     2941 gcggaatacg cgttaaatcg ctccaggttc ccagcacacg gttaaagtcg atatcgacaa
     3001 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     3061 aactgtagcg ggaaaggatg ggtttggcta tgtggcaata agggtaatct tgtgatgctg
     3121 attgcgaaaa agcagagtaa tttcactggc tttttcgcaa tagatgaata gttaagattc
     3181 gggtgtaaat tgccaacgag tttcgatgac cagcgccgat atgacaccgc aggatattta
     3241 tccacttgtc gcgcttactg atcatgccgt gttctcaacg ccagggcgga aacgcatgac
     3301 tggcctgcag tcacctgaag aaggtttgta ccaggcaacg acacaaactg cgggtaaagt
     3361 gtatgtcacg ctgggttctg aagattcatt gtggattaaa gatgatcatt tgtgtcaaca
     3421 ggatgcattt tctgtcaatg tggttgatac gacaggagca ggcgatgttt ttcacggcgc
     3481 ggtagcgttg gcaatgcacg gaatgtcagg gcttttttta ctgatgcccc gcctcccaat
     3541 tctttctgcc agttgttaag tgaagagaat gttgaactgg tagttgccga gcaagaagta
     3601 tcctgaaatt gaaacttcga aaaatccgct ggggattcag cggatttttt ttggccggat
     3661 taagattaag cacatccggc cattaattaa gcttcagccg ccagcatctc tttcttacgt
     3721 tggctaatac gcttaaccag cattacggaa accaccgcac cacagaatgc gaagcaggcc
     3781 ataatgccaa acacgatttt gtagccaata atccccggat ttaaatccag aatgtagcca
     3841 tacagactga agcagaacat cgccggggcg taaccaataa agctccccaa cgccatcgcc
     3901 gcgcctgttt tattttcagc aatttttgct tcgccgatag gtgcaaaaaa tacggctcgc
     3961 tgtgtaaaga ctatcgcgcc aaagcccagc gtacatgcca tccctaaata gaccggcata
     4021 ctttcgtgcg gcagcataat caacagtacg agcgcagcgg tactgatgat aaaggtgtag
     4081 catagatatt tactcggcga tttcaggatc ttatctgaaa tcatgccacc aatcggtcca
     4141 ccaatcattt tcagacagta ttggttaatg atgccgtaag cccccaccag cgcaacgggc
     4201 aatagataga tgtttttcag gaatggaatg aagaatgtca ggccacagta aaccgcgtaa
     4261 acgaagaaga cgttgaaagc gataagccag atggttttgt ctctcagcac cgaggtcata
     4321 ctggtgtttt gcgatgcccc gtcttctttt ttcacctcaa cggacggtgc ctcttctttg
     4381 tcattcagga caaagaaaat aataatgcct acggcaatca caataaggga atagaaccag
     4441 atgcctgctt taaaacctaa taagccactg ccaaaccagg taaatactgc caacgcagaa
     4501 aatgccacca cggtatcgac aatgccacgc cctgtttcga agaagccaaa caaccgacct
     4561 tgttgttcgc tattgcccaa tcgacttacc gacttcagca agaccggcca gttcatcatg
     4621 tcgcaagtaa caccaaatag cgcccagaca aagaggatgc cccaataccc cggcattgtc
     4681 gtcaaataaa cacccagtaa tcctgtcgca atgagtgaga atgacatggt gtatctgcgc
     4741 ggtagtttat cggcaaaata aatagacaga aaaaagccca ctgtggtgac aaatgagttt
     4801 accgacatag cattaccaat ttgaccattg gtcaaatgga aatattcctg catcgggata
     4861 taaaacgcat ctttcagcga cggtaattta taaattgtac cgccacacag tgttaataga
     4921 ctaaataacg cccatttctt tttcgtgagc ataacatccc ctaagcgatt tttaaaatca
     4981 ctgttttaaa ggcaataaga taccgaccat tcatatggaa tggttttatt aactgcgtat
     5041 gagttaaaac ttattagtgg ttcagaagat ggtttgcctc ttgtttgatt tgttgtagta
     5101 cgttacgcac atgatttatc tgattaattg catcttcttt ttctttacgt aaccgcgcat
     5161 ccagtaaatg attttctggt aggggtgttt cactcatctg gcgataaggg atccacggat
     5221 tatcatcttc gtcttcaaag cggaacgccg gcgcatagta atcaacctct tcttccaggc
     5281 catatgccgt cacctgcggc tcatcatcac caaggcagat aaggtgcgtt aataacgctt
     5341 tgaacggcat atcaccctga cctgaaatac aggctttatg acccaggcca ccctgttctt
     5401 taacgatcaa ggcatcttta atatggacct gggtaatatg cggtgccatc gtttttaagg
     5461 cgtcgatggg atgctcattt gcattgatca tgttcgcaaa atcaaacagt aaggaaagag
     5521 actccatctc actttctttg accagcgaca ccagctcatg actttttaaa tcttcatgct
     5581 gctcaatcgt aaaagtcagg ccgctgtcct gatacgtttc ccgtacatag gcaatgtcgt
     5641 tagcgataat cgataatacg tcgcgcaaat taccttcata acgtggataa aaacgtacgg
     5701 acgatgcccc agttttcaac gcgatggcga cggcttcgtc aatagatgcc ttatctgagg
     5761 cgctggtttc aatatgaata tcaaggttca gacggcgggc tttatctcca aaggcagaga
     5821 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
     5881 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
     5941 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
     6001 gtagaatctt tcttgcgtta tttattgtca tcataaaaca cctacagatt aatgatgagg
     6061 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
     6121 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
     6181 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
     6241 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga
     6301 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc
     6361 cagccggtga tgcgatagag ttcagttgat ggtgtcactg caatcagtgg cgcgagcgtc
     6421 tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aggaaggctc tcgcccttgc
     6481 tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg
     6541 gtcacgaacc accccttacg atctttacgg aagattttgg attctgcctc gaggttcaac
     6601 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
     6661 gggagttttc cacccggagt ggtgatgcca tctttcaacc accgatccag tttctctttt
     6721 gcattttcta cggtggattg attctccgcc attgtttaat ccttttggtt taaaccagat
     6781 gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata
     6841 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
     6901 tgtgcgctca cgacaggcaa aaaaaaacct gccatcgctg gcaggttttt tatgactaaa
     6961 aaacgaaatt aatcgtcttt cggtgcgcgg ttagcgcgac gacgatcgtt ttccgtcagg
     7021 tgacgtttac gaatacggat agaggtcgga gtcacttcta ccagttcgtc atcatcgatg
     7081 aactccagag cttgttccag agtcatgcgg ataggcggaa ccagaacaac ggcttcgtca
     7141 gtaccggaag cacgcatgtt ggtcagtttc ttaccggtca ggcagtttac agtcaggtcg
     7201 ttagagcggc tatgaatacc gataatctga ccttcgtaaa cttctgcacc gtgaccgagg
     7261 aacagcttac cgcgatcctg cagaccgaac agcgcgaacg cgaccgcttt accctgaccg
     7321 ttagagatca gtacaccgtt ctgacgctga cccacttcgc ccggacgtac gtcgtcgtag
     7381 tggctgaagg tggagtacag cagaccagta ccggaagtca tggtcatgaa ctcagaacgg
     7441 aagccaatca gaccacggct tgggatcacg tagtcgagac gtacgcggcc tttaccgtct
     7501 ggattcatgt ttttcaggtc gcctttacgt tcgcccagcg cctgcattac agaaccctga
     7561 tgctgttctt caacgtccag agtcacgttt tcatacggct cttgtttacg accgtcgatt
     7621 tcacggaaga taactttcgg acgggatacc gccagttcga aaccttcacg acgcatgttt
     7681 tcgatcagaa cagacaggtg tagttcgcca cgaccagaaa cgcggaacgc atcagcgtct
     7741 tcggtttctt ctacgcgcag cgcaacgttg tgtaccagtt ctttgttcag acgatccagg
     7801 atctgacgag acgttacgaa cttaccttct ttaccgcaga acggcgaggt gttaacgcag
     7861 aagaacatag aaacggtcgg ctcatcaacg gagagtgccg gcagcgcttc aacgttttgc
     7921 gtgtcgcaaa cggtgtcaga aatgttcagt tcgcccaggc ccgtgatcgc cacgatatcg
     7981 ccagcttccg ccagatcggt ttcgatacgt tccagaccga ggtggcccag cactttaccg
     8041 actttcgcgt tgcgggtttt gccttcgcta tcgatgatag taacctgctg gttcggcttc
     8101 actttaccgc gcttgatgcg gccaatgccg ataacgccaa catagctgtt gtaatcgagc
     8161 tgagaaatct gcatctggaa cggaccgtca aggtcaacgt ccggcgcagg aacgtggtca
     8221 acaatcgcct ggtacagcgg ggtcatgtct tccgccatat cttcgtggtc cagacccgcg
     8281 ataccgttca gcgcagaagc gtaaacgatc gggaagtcca gctgctcgtc ggtcgcgtcg
     8341 aggttaacga acagatcgaa tacctgatcc acaacccaat caggacgcgc gccagggcgg
     8401 tcaactttgt tgataacaac aatcggcttc aggccgtaag caaacgcttt tttggttacg
     8461 aagcgcgttt gcggcatcgg gccgtcaaat gcgtcaacca ccagcagcac tgagtctacc
     8521 atggacatta cacgttcaac ttcaccaccg aagtcggcgt gccccggggt atcaacgatg
     8581 ttgatacggt aatcattcca tttgatagcg gtgtttttcg cgaggatggt aatcccacgc
     8641 tctttctcca aatcgttgga gtccatcacg cgctcttggg tttcggcacg agagtcgaac
     8701 gtaccggatt gttggagcag cttgtctacc agggtggttt taccatggtc tacgtgcgcg
     8761 atgatggcga tattacgcaa tttttcgatc acaactttgc ctcaggcatt agaaatagcg
     8821 cgttattgta cacggattaa tcgcactaca aaacaggatc acaaacatcc tccgcaaaca
     8881 agtattgcag agtccctttg tgatcgcttt cacggagcat aaaaagggtt atccaaaggt
     8941 cattgcacca atatagtgct taatgtttcc attgaagcac tatattggtg caacattcac
     9001 atcatggtgc agcccttttg cacgatgtta agcatgataa cgccttttag gggcaattta
     9061 aaagttggca cagttttcgc tttatctttt ttacggcgac acggccaaaa taattgcaga
     9121 tttcgttacc acgacgacaa tgaccaatcc aggagagtta aagtatgtcc gctgaacacg
     9181 tactgacgat gctgaacgag cacgaagtga agtttgttga tttgcgcttc accgatacca
     9241 aaggtaaaga acagcacgtc actatccctg ctcatcaggt gaatgctgaa ttcttcgaag
     9301 aaggcaaaat gtttgacggc tcctcgattg gcggctggaa aggcattaac gaatccgaca
     9361 tggtgctgat gccagacgca tccaccgcag tgattgaccc gttcttcgcc gactctaccc
     9421 tgattatccg ttgcgacatc cttgaacctg gcaccctgca aggctatgac cgtgacccgc
     9481 gctccatcgc gaagcgcgcc gaagattacc tgcgttccac tggcattgcc gacaccgtac
     9541 tgttcgggcc agaacctgaa ttcttcctgt tcgatgacat ccgtttcgga tcatctatct
     9601 ccggttccca cgttgctatc gacgatatcg aaggcgcatg gaactcctcc acccaatacg
     9661 aaggtggtaa caaaggtcac cgtccggcag tgaaaggcgg ttacttcccg gttccgccgg
     9721 tagactctgc tcaggatatt cgttctgaaa tgtgtctggt gatggaacag atgggtctgg
     9781 tggttgaagc ccatcaccac gaagtagcga ctgctggtca gaacgaagtg gctacccgct
     9841 tcaataccat gaccaaaaaa gctgacgaaa ttcagatcta caaatatgtt gtgcacaacg
     9901 tagcgcaccg cttcggtaaa actgcgacct ttatgccaaa accgatgttc ggtgataacg
     9961 gctccggtat gcactgccac atgtcgctgt ctaaaaacgg cgttaacctg ttcgcgggcg
    10021 ataaatacgc aggtctgtct gagcaggcgc tgtactacat tggcggcgta atcaaacacg
    10081 ctaaagcgat taacgccctg gcaaacccga ccaccaactc ttataagcgt ctggtcccgg
    10141 gctacgaagc accggtaatg ctggcttact ctgcacgtaa ccgttctgcg tctatccgta
    10201 ttccggtggt ttcttctccg aaagcacgtc gtatcgaagt acgtttcccg gatccggcgg
    10261 ctaacccgta cctgtgcttt gctgccctgc tgatggccgg tcttgatggt atcaagaaca
    10321 agatccatcc gggtgaagcc atggacaaaa acctgtatga cctgccgcca gaagaagcga
    10381 aagagatccc acaagttgca ggctctctgg aagaagcact gaacgagctg gatctggacc
    10441 gcgagttcct gaaagccggt ggcgtgttca cggatgaagc gatcgatgcg tacatcgctc
    10501 tgcgtcgcga agaagatgac cgcgtgcgta tgactccgca tccggtagag tttgagctgt
    10561 actacagcgt ctaatagttg aagttgtact acccggcgca acaacgccgg gatttagttg
    10621 ccgtggaaac tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct
    10681 tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga
    10741 ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa
    10801 cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccccg ccgcgcaaca
    10861 actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta
    10921 cttctcatta aatattgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga
    10981 taacgaagtg acgctggtga tcgacggtcg ctcgcatatc ctttctgtga cggcccagcg
    11041 tatgccggac ggcatgatcc tgctggagat ggcaccgatg gataaccagc gccgcttaag
    11101 tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc
    11161 acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctac tcagcaaagc
    11221 attacctgac ccgtcactac tggaatacac caaagtgatt atcgaacagg cggaccggct
    11281 gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg tgaccgaaag
    11341 tattcacaaa gtagctgaac gcgtggtgac gctggtgtcg atggaactgc cggacaacgt
    11401 gcggttgatt cgtgattacg accccagcct gccagaactg gcgcacgacc cggatcaaat
    11461 tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg
    11521 tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg aacgctatcg
    11581 gctggcggcg cggattgatg tggaagataa cgggccaggc attccgcctc atttgcagga
    11641 tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat
    11701 cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccagggca
    11761 taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg
    11821 atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct
    11881 ggagcgggtt taacctgtac gacatttgag aacggcgcgg aggtactgga ggcgctggcg
    11941 agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg
    12001 ctgctcaagc agattaaaca gcgccatccg atgcttccgg tcatcattat gaccgcacat
    12061 tccgatctgg atgctgccgt cagcgcctat caacaagggg cgttcgatta tctgcccaaa
    12121 ccgtttgata tcgacgaagc cgtggcgctg gttgagcgcg ccatcagtca ttaccaggaa
    12181 cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggcgaagcg
    12241 ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg
    12301 ctgattaacg gcgaatccgg taccggtaaa gaactggtcg ctcatgccct gcatcgccac
    12361 agtccgcgag ccaaagcgcc gtttatcgcg ctcaatatgg ctgctatccc gaaggatttg
    12421 atcgaatccg aactgtttgg tcacgagaaa ggcgcattta ccggcgcgaa taccattcgt
    12481 caggggcgtt ttgaacaggc tgatggcggt acattattcc tcgatgaaat tggcgatatg
    12541 ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag atggtcagtt ttaccgcgtt
    12601 ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctt
    12661 gaacagcggg tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc
    12721 cgcgttcatc tgccgccgct gcgtgagcgt cgggaagata ttccccgcct ggcacgccat
    12781 tttttacagg ttgctgcgcg cgaactgggc gtagacgcga agttgctgca tccggaaacc
    12841 gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc
    12901 cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa
    12961 ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag ttgggcgacg
    13021 cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa
    13081 gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat
    13141 aaacaggaag cggcgcggct acttggctgg ggccgtaaca ccctgacgcg taagttaaaa
    13201 gagctgggga tggagtgatt cacggcttgt gtgtaaagat tgattattga gcgcaaattg
    13261 ctggtatttt gcgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg
    13321 agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac
    13381 acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcga tgattgggct gtttagttga
    13441 gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga actccttatc
    13501 cggtctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag
    13561 gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc
    13621 attcactatt taaatcaccc gagagaactg ctgcatccgc gctttctggc gcagataggt
    13681 atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc
    13741 cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc
    13801 cgcaaagtaa tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg
    13861 gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc
    13921 aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact gtttcaactc
    13981 tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag
    14041 cagatcggta tcgccctgag tggtgtagcc ctggaaatta cgatgcagca cgccttcacg
    14101 ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata
    14161 gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact
    14221 cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca aatgcgcgta
    14281 gttaaagacg ctcagacggt cagggtttag ctccgccacg cgtttcaggg taaaggcgaa
    14341 actttccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaagcc
    14401 aatctcacgc gcatggttaa gcagcgcaaa gataaactct tcatcctgct cgcggttaac
    14461 cagacgttgc acttctttgt tgaagtcctg cacgcccata ctcaggcgat taaagccctc
    14521 ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat
    14581 ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg
    14641 cgctttatta agatacgtcg gcgttccgcc gccccagtgc agctggctga cgtgacgccc
    14701 ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc
    14761 cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca
    14821 gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa
    14881 aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg
    14941 tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga
    15001 catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt
    15061 cgggtcataa gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg
    15121 aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg
    15181 gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct
    15241 tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga
    15301 tccagtttag catccaccca ggattgctct tcggcactca gggtttcgcc tgcttccaga
    15361 cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc cgcctgcggt
    15421 gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc
    15481 aatggaatag gggttttact gccaatacgt ggatctttcg gtgcgttctg gcctttgctg
    15541 cctgacgtgt tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc
    15601 tgacgcttgc ggtcacgagc ttcctgattc agctcttcgc gcgttttgcg acgcgctttt
    15661 gcatgacctt tgctgcgtga tggtgaagat ggtttcataa tggttcgtct tttagccgtt
    15721 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    15781 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    15841 ggccgccaac acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttccg
    15901 tttaattacg tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    15961 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    16021 tacgcctttg ctttaaacaa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    16081 tatataaaag caattaactg atttatatag cttatttgac atgcatcgca gaattttctc
    16141 tgctatttcg cataaagact atcggcaatg tctcacaaag cgtatggctt ttacttacaa
    16201 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgccg caaagctttg
    16261 actgacggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    16321 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    16381 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    16441 tacctcaaaa accccaggac gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    16501 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    16561 atggcagcgc gcgctcggcg agtacctcga aaaacgtcag agcctgcaag gtctggtggt
    16621 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    16681 agacagcaat atcgccgtgc tggtgttgct gaccaaagcg gacaaactgg caagcggcgc
    16741 acgtaaagcg caattgaata tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca
    16801 ggttgaaacg ttttcttcgt tgaagaaaca aggcgtggac aagctgcggc agaaactgga
    16861 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    16921 ccttaatgct tgtgccggat gtgacgtatc cggcccataa attcaataga ttacaattct
    16981 accgttggcc tgataaacat ggcgcatcag gcattacgga tcttttcttt cgcccaataa
    17041 aaaacgcccc actcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt
    17101 gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac
    17161 tcttttttgt tcagttcaga aagcactttt tgtaattttt tttcagttgt tgcataggaa
    17221 tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat
    17281 gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca
    17341 acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat
    17401 cgacatcatc tttatggact tcaaatacca gttcatcgtg tacctgcatg atcatacgta
    17461 cacgcggttg ctcagcctgt aaccacgcat caacggcaat catcgcccgt ttgataatgt
    17521 cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag
    17581 caccattgct ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt
    17641 agccctgctc tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc
    17701 gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg
    17761 ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacggc
    17821 gttgctcgct ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcccggt
    17881 ggatatcttt tccttccgcg aatgcggtca gcaagccttt gtcacgcgag agatgcgcca
    17941 taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa
    18001 taaacgcctg gcggatacga cgaccttctt cgttacgcac cggaatgttt tgcaggttag
    18061 gatcggttga cgataaacgt cccgttgcag ttactgcctg gtgataagag gtatgcacac
    18121 gcccggtttt cgggttgatc atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg
    18181 ccagaccacg atactccaga atcacttttg gcaacggata gtccagcgcc agttcttcca
    18241 gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt ttaatgccct
    18301 gtttttcaaa cagaatggtt tgtaactgct tggtggaaga aaggttaaat tcctcacctg
    18361 caatttcatg cgctttcttt tccagctcag ccagacgaag ggtgagctct tcagaatgat
    18421 tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca
    18481 ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg
    18541 gccacatttt cagatgcaac tgcaaggtga catctgcatc ttcggcggcg taacgtccgg
    18601 cctcttcgag ggcaatctgg ttaaaggtca gttgattttt gcctttaccc gcaatctctt
    18661 caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc
    18721 cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagct
    18781 caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg
    18841 ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgcga gagatttgat
    18901 cgggcgcatc aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag
    18961 caaaagaaag cccgaccagg ttagcagaga tgttatcgag gctgtcggtt tcggtatcaa
    19021 atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa
    19081 ggatggtgac gtagttgtca taagaaatca ccgttgccgt cacttctggt gcttcgtctg
    19141 caacactggt ttcctgtggc ttcgcggcgg gttttgcccc tttggcctgt aaccatttgc
    19201 ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc cccaacaact
    19261 cttccgctgc cggttgctgc acttccagtt gttcacaggt cagctccagt tcaacgtcgg
    19321 ttttaatcgt cgccagctgg tatgagagat aagcaacttc tttgttttgc tcgagcttcg
    19381 ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc tcggcataca
    19441 gcgtatccag cccgccaaga ccttgcagca atgcctgcgc ggttttttca ccgacgccgg
    19501 gtacgccagg aatgttatca gaggagtcac ccatcagcgc caggaaatcg atgatcagtt
    19561 ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg
    19621 tattgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctga
    19681 tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg
    19741 cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcaagg
    19801 gttcgatttg tgcacgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa
    19861 acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt
    19921 tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct
    19981 cgcctgcgct gttagtcagc ggaggaaacg cgtgatatgc gcgataaaga taagatgaac
    20041 catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc
    20101 agattatggg taaaggatgc cacagacagg ataaaaacat gagtttttca ccgcttttcg
    20161 tgaaaatctt ttgtagatct tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc
    20221 ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg atgtttgtct
//
LOCUS       DAZUGK010000073        20240 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZUGK010000073 DAZUGK010000000
VERSION     DAZUGK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.073"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..841
                     /locus_tag="ACHY5W_002302"
     CDS             26..841
                     /locus_tag="ACHY5W_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4447192.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TPLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            843..983
                     /locus_tag="ACHY5W_002303"
                     /pseudo
     CDS             843..983
                     /locus_tag="ACHY5W_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            1085..1558
                     /gene="ysaA"
                     /locus_tag="ACHY5W_002304"
     CDS             1085..1558
                     /gene="ysaA"
                     /locus_tag="ACHY5W_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS4447193.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAVKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1597..2850)
                     /gene="avtA"
                     /locus_tag="ACHY5W_002305"
     CDS             complement(1597..2850)
                     /gene="avtA"
                     /locus_tag="ACHY5W_002305"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFS4447194.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQMLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3028..5058)
                     /gene="malS"
                     /locus_tag="ACHY5W_002306"
     CDS             complement(3028..5058)
                     /gene="malS"
                     /locus_tag="ACHY5W_002306"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS4447195.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVANQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDVPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEVWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            5378..6202
                     /gene="bax"
                     /locus_tag="ACHY5W_002307"
     CDS             5378..6202
                     /gene="bax"
                     /locus_tag="ACHY5W_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFS4447196.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(6310..7488)
                     /gene="xylR"
                     /locus_tag="ACHY5W_002308"
     CDS             complement(6310..7488)
                     /gene="xylR"
                     /locus_tag="ACHY5W_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFS4447197.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7566..8747)
                     /gene="xylH"
                     /locus_tag="ACHY5W_002309"
     CDS             complement(7566..8747)
                     /gene="xylH"
                     /locus_tag="ACHY5W_002309"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFS4447198.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKALNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8725..10266)
                     /gene="xylG"
                     /locus_tag="ACHY5W_002310"
     CDS             complement(8725..10266)
                     /gene="xylG"
                     /locus_tag="ACHY5W_002310"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFS4447199.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMLPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10344..11336)
                     /gene="xylF"
                     /locus_tag="ACHY5W_002311"
     CDS             complement(10344..11336)
                     /gene="xylF"
                     /locus_tag="ACHY5W_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4447200.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            11702..13024
                     /gene="xylA"
                     /locus_tag="ACHY5W_002312"
     CDS             11702..13024
                     /gene="xylA"
                     /locus_tag="ACHY5W_002312"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFS4447201.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            13096..14550
                     /gene="xylB"
                     /locus_tag="ACHY5W_002313"
     CDS             13096..14550
                     /gene="xylB"
                     /locus_tag="ACHY5W_002313"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4447202.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWIQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGGFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            14719..15060
                     /gene="yiaB"
                     /locus_tag="ACHY5W_002314"
     CDS             14719..15060
                     /gene="yiaB"
                     /locus_tag="ACHY5W_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFS4447203.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            15106..15525
                     /gene="yiaA"
                     /locus_tag="ACHY5W_002315"
     CDS             15106..15525
                     /gene="yiaA"
                     /locus_tag="ACHY5W_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFS4447204.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVT"
     gene            complement(15585..16580)
                     /gene="wecH"
                     /locus_tag="ACHY5W_002316"
     CDS             complement(15585..16580)
                     /gene="wecH"
                     /locus_tag="ACHY5W_002316"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFS4447205.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGEKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16755..17054
                     /gene="ysaB"
                     /locus_tag="ACHY5W_002317"
     CDS             16755..17054
                     /gene="ysaB"
                     /locus_tag="ACHY5W_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFS4447206.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            17149..18060
                     /gene="glyQ"
                     /locus_tag="ACHY5W_002318"
     CDS             17149..18060
                     /gene="glyQ"
                     /locus_tag="ACHY5W_002318"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4447207.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            18070..20139
                     /gene="glyS"
                     /locus_tag="ACHY5W_002319"
     CDS             18070..20139
                     /gene="glyS"
                     /locus_tag="ACHY5W_002319"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4447208.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         5026 a         4947 c         5186 g         5081 t
ORIGIN      
        1 tgaataagcg ggaaattaca tatcaatgct cgaattatcc atgacgttac cgattaaagt
       61 gcaaaatggt ggattattta tttcccgtgg cgtagggagg catcctgcaa ggcggctgga
      121 gtcatgggaa attatctttg tcgaaaaagg tcgattaacg attcaggaag aagaacggat
      181 atttctggtc gatgcgggag agagtttatt actctggccg aaccgacaac atgttggggt
      241 ggaggatttt cccgccgatc tcaaatttta ctggctgcat tttgaggtta aagcgccgga
      301 caacgaccct cgctggctta ctcatcttag cgtgccgcaa caaacgaagg ttgtcgatcc
      361 gcaggcgctg gtggccctgt ttcgccagtt cttaagcgaa caggaaaagc atcaacgcac
      421 tccggcgctg gagtttattg tgctgctcat tttgcagcag ctcacccgtg acgcgcgtct
      481 tgatgaacag gccgatggcg caggcacccc ccttgcctgg aaagcgcaga aactgatccg
      541 cacgcaatat catcgcccgc tttctacggc gcttctggct cgcgagctgt actgcaatgc
      601 cgattatctt gggcgcgtct accggcgggt gtttcatctg acgttaacag aagcaatcca
      661 tcgccagcgg gtgcgcgaag ccgaaaagct gttaattagc gaagcgcgat cgctcacgga
      721 agtggcggcg ttgtgcgggt ttaatgacgt tggctatttc cggcagattt ttcgcaaaca
      781 caccggttta acgccagcag cgtggaagcg gcgttacagc aaggaacata tcaattcgta
      841 gtgccggggc gatttcgctt tcgacatcac gtctgaaaca ggtgggggag aaaaatctcc
      901 tgaaaccact gcgtgaaacc gcgcaggcta tttctaatga actggggttt actgtcaggg
      961 acgacctggg cgcgatcaca taacgctttg gacaagtgcc aaaactttaa catttccttc
     1021 gttggatcaa agcagtaggg gcgcgttctc tggcactctg ctgttttagt gcaaaggggt
     1081 gatcatgaac cggtttatta ttgcggatgc gacgaaatgt atcggttgcc gtacctgtga
     1141 agtggcttgc gcagtgtcgc atcaggaaaa tcaggattgc gctgcgttgt caccagacga
     1201 gtttatttcc cgtattcgtg ttattaaaga ccacagctgg accacggcag tagcctgtca
     1261 tcagtgtgaa gatgcaccgt gcgcgaatgt ctgccctgtt gacgcgataa gccgcgaaca
     1321 tgggcatatt ttcgttgaac aaacacgttg cattggctgt aaaagctgta tgctggcttg
     1381 cccgtttggt gcgatggagg tcgtttcttc gcgcaaaaag gcgagggcgg ttaagtgcga
     1441 tctgtgctgg catcgggaga cgggaccggc ctgtgttgaa gcctgcccga caaaggcgtt
     1501 gcagtgcatg gatgtcgaga aagtgcagcg gcatcggcta cggcagcagc ctgtttgaaa
     1561 cgtttttccg gcgtttctca acgccggaat agcgtattaa tggctttcag cccaggcgcg
     1621 ttcaatctct tccgccagaa tcttcacacc cgcctcaatt ttctccggct ctggtacgta
     1681 gttcatacgc atacattgat gcgtatgcgg ccacggttta tccagccctg ggaagaagtt
     1741 gtgccccggc accatcagca cgccgcgtgc tttcaggcgc tggtagagct gctcggtcgt
     1801 aatgggcaaa tccttaaacc atagccagag gaaaatggct ccttccggtt tatgaatcag
     1861 gcagcgattt tccggtaaat agcggcgaat gatggcgata gtttcctgaa cacgctggta
     1921 gtaaaacggt ttgatgactg tttcagacag gcgcagcaga tcgttacgct taatcatttc
     1981 acacatcatc gccggaccaa taccgccagg tgccaggcta ataatgccgt tcatattggt
     2041 gatggcggtg atgatttttt cattggcgat gataatgccg cagcgggagc caggtagacc
     2101 cagcttggaa agactcatgc acagcacgat attcggattc catagcgggc gcgcttcact
     2161 gaagatgata cccgggaacg ggacgccata agcgttatca attaccagcg gaatgccgtg
     2221 ttgattcgcc agcgcgtcaa gcttcagcaa ctcttcgtca gtaatcacat tgcctgttgg
     2281 attcgtcggc cgggagacgc aaatcatccc ggtttcttcg ccaatatgca gatgctcaaa
     2341 atcgacgtgg tatttaaact ggccttcagg cagcagttca atattcggac gcgcagagac
     2401 aaacagatct tcttccagtc cggcgtcagc gtagccaatg tattccggtg ccagcgggaa
     2461 cagcactttt ttgacccgac catcggcacg gcgtccggca aacagattaa ataagtagaa
     2521 aaacgcgctc tggctgccgt ttgttagtgc aatattctgt ggttcgatat cccaacccaa
     2581 cttctcgcgc agcattccgg caagcagtgt gagtagctcc gttttcccct gtggaccgtc
     2641 gtaattacac agtgcatcag tcgctttgcc actttccagc atgtcggtca gtagcatctg
     2701 gaagtagtcc tgcatttccg ggatctgcgc cggattaccg ccgccgagca taatcgcgcc
     2761 aggcgtgcgt aaaccgtcgt tcagatcttc catcagcagc gtaatgccgg agtggcgggt
     2821 aaatttgtca ccaaaaaggg agaatgtcat agcgggatat ctgtcgaact ctgtagcaag
     2881 gaaggtaaca ataacgctac atttgctggg gtgcaaatcg gtctgttgaa gggtgagtgg
     2941 tgctttatcc tgcaacgctg attagggctg acattttatc ctggttggcg gcttcccgag
     3001 gtactgacga acgggaagcc ggaaaagtca ctgttgccca gcccagatga ccagcacttt
     3061 atcgtcgcca tgctcacgaa caaagccgta gccctgcttc agcgaaagtg tcgtttgttt
     3121 gcccgcgcca attgcgggat ggcgggcgcg gaactggctg attttctgcc agtgcgcgac
     3181 gctggcggca gatttaccgc taacatcctg ccagttcata tccgaacgtg taccttgcag
     3241 cggatcagaa cctgtaggac cgaacggacg cgaggattca tcaccataaa agatttgtac
     3301 cgcgcctggc gctaatagta ataactctgc tgctttgtcg cctccttcac ggaacaggcg
     3361 ggtatcatgc gacgagaggt agctcaacac gttgaaaccc tgcaatttct ccgccatttg
     3421 ctgccaggtc gtatccatct gcgccagaca atcgactgct ttcgccgcct gctcctgata
     3481 atcgaaattg atcatcgcat cgaagccgtg gcgatagtag tcactttgca tcacgccgtg
     3541 gccccagact tcaccggtca tccagaaagg tttgtcatct aatgctttgt cggggttagc
     3601 ttttttccat tcgcgaagcg cggcgctggc ttcggttttc agttgctgcc aggcgggcaa
     3661 ctcaacatgt ttggcggtat cgacccgaaa accatcaatc ccatagtcgc ggacccactg
     3721 acttaaccag tgggttaagt aatcgcgagg tgtaaagcct tcgatgactt tagcgtgagt
     3781 atccgttttg tttttataga acaccggcag accagaagcg gtagttgatt cggttttgat
     3841 atccggcaaa aaggccagcg acatagtgag atcgtcaaat ccaggattgt cgtaatcgcc
     3901 gatatccgtt ctgatccagt tttttcccca ccatttatcc cagcctgttt tgtcgctgaa
     3961 attaatgtaa tcgttaaagc tatgccaggt ttgcccggcg gcaggtttcc agtcgctcca
     4021 gcgttcaccc agcgtttttt tcacttcgtc accagaaaga tataacgcgc caaactgata
     4081 ctcctgcata tccgccagcg tggcatagcc ggtgtggttc atcacgacat caaagagaat
     4141 acgaataccg cgctgatgcg cgctatcaac cagcgtccgt agatcggctt cgctgcccat
     4201 attggcatca agattcgtcc agtcctgtgt gtaataaccg tggtaggcat aatgcgggaa
     4261 atcacctttt gtaccgccgc cgacccagcc gtgaatttgc tcaaatgggg cgcttatcca
     4321 caaagcattg acgcccagct gctggagata atccagtttg ttggtcaggc cgcgtaaatc
     4381 gccgccgtga aaagtaccta tttctgccat accgtcttta tgacgtccgt aactctggtc
     4441 attactggga tcgccgtttt cgaaacgatc tgtcagcaca aagtaaaccg tggcgttatg
     4501 ccagtcgaaa ggggcaggga cgtcagtttc tgcccgttcc agcaggagta aaccgttgct
     4561 ggtggcagcg ggttgtaacg ttatttgacc gttcttcact atcgcaattt gctggctgta
     4621 ataatcccgt acggcggctc cttccggaaa agtggcgctg acatccagcg tgagcggtaa
     4681 tccatcccat ttcgggcata cacggacctg gtttgctacc ggttcggcgg cgttctggat
     4741 ggaaatcatc aatgttggcg tgccggagcg ggtgtctatt tgcagcgtat attcgccgtc
     4801 cctgaacaat cgccattgag gcggcgtgtt gctacaaggt tgcagggaaa gcatctgatt
     4861 gagttttatc gcatccgcag gctgccagca ctgttggtca aaatttagcg ttagtggacg
     4921 cgtacctttg ggcaactgcg cgtggctgac aaatgttccc gttccctgtt cactaaaggc
     4981 agggaacccc ggagaagtcc agctggcggc aacggcgaag ccaggaagga gtgtcagaaa
     5041 acaggcggcg agtttcatgg cggatgagtc cttattgctg cgatttcaga cagtttgcca
     5101 gcgataagcc aaacaaaact catccctgag cggtaagtcc acaggatgag aagcaagggt
     5161 gagcgatctc gcgcaaaaaa cggctgaatt ttgcgataaa ccccacattt tctgcgattt
     5221 agcgccaatc tgaatcgtta acacgtgaca tagtttcaga tttggactat tccttggggt
     5281 gctcttgaca gctatttttg atatgatttg agattcaact ctcaaatttg tgaaaaatat
     5341 aaggtgttgg aatgattaaa tccgaccagg agatctgatg attttgactc ccatacgacg
     5401 atatggggcg atgattctta tgttactcac tctggtgttt tcgagtgagg tgttagcgaa
     5461 gacgcacaca acaacagcga gtcaaaagtc ccacttaact aaagctagta ataaacaggt
     5521 aagcagtaaa caagagtatt ctcgcaatag tgcaaagagt agttcacttc ctgatttgcg
     5581 aaaataccct tccggaacac caaggaaaaa ggcgtttctc cggaccgtaa tgccttacat
     5641 taccagccaa aatgcagcca ttactgcaga acgtaactgg ctgatttcaa aacagtatca
     5701 gggccaatgg tcacctgctg agcgtgcgcg tctgaaagac atcgccaaac gctacaaggt
     5761 gaagtggtct ggtaatacgc gaaaaatccc gtggaatacc ttgcttgaac gcgtagacat
     5821 tatccctacc agtatggtgg cgacgatggc tgcagcagaa agcggttggg gaacgtcgaa
     5881 gctggcgcgc aacaataaca acctgttcgg catgaaatgc atgaaaggac gttgtaccaa
     5941 tgcgccaggt aaagtgaaag ggtactcaca gtttagttct gtcaaagaat cggtgagcgc
     6001 ctatgtcact aacctcaata cgcatccggc ttactcttcg ttccgtaaat cgcgtgcgca
     6061 gctgcgtaaa gcggatcagg aagtgactgc cacagcgatg attcacaagc tgaagggcta
     6121 ctcgaccaaa gggaagagtt ataacaacta cctgttcgca atgtaccagg ataaccaacg
     6181 gttaatcgcg gcgcatatgt aattgtattt cctcgagcct tatccgaatt gtcggtcgga
     6241 taaggctttt gatcgcatca ggtggttgtg ctgagttacc tgatgcgacg ccgacaattc
     6301 tcatcatcgc tacaacatga cctcgctatt tacatcgcga tactcttttg gcgtcgtgtc
     6361 atatgctttt ttaaaaacag agtagaaata ttgcagcgat ggataaccgc acatttgcga
     6421 tatctcattg atcgacaagg tggttgaaat cagcagactg cgcgctttct ccagcttctc
     6481 ggcatgaatc atggcatgga tggtttcacc cacctcttct ttaaaacgct tctcaagatt
     6541 ggagcgcgag atcccgaccg catcgagtac ctgatccact ttaatccctt tacaggcgtg
     6601 attacgaatg taatgcatgg cctgaataac ggcgggatcg gtcagcgaac ggtaatctgt
     6661 tgagcgccgt tcaatgacgc gaactggagg gaccaaaatc cgctgtagcg gcatttcttc
     6721 tttatctaat aatcgatgca acagttttgc cgcctgatag cccatttgcc gcgcgccctg
     6781 agcgaccgaa gaaagggcga cacgcgacag atagcgggtc agttcttcgt tatcgatgcc
     6841 aatcacgcat aatttttccg gtacgggaat gtgtagatgt tcacatactt gcagaatatg
     6901 ccgcgcccgg gcgtcagtaa cggcaataat cccggtttgc ggtggcagcg tttgtagcca
     6961 gtctgccagc cgattttgcg cgtgttgcca gttctctggt gcggtttcta acccctgata
     7021 aaccactccg cgatactttt cttcggcgac aagctgacga aatgcatatt cgcgttcagt
     7081 ggcccaacgt ttgccgcttg attccggaag accataaaaa gcaaagcggt taacgccttt
     7141 ctcttttaaa tgcaaaaatg cgctttcaac cagcgcatag ttatcggtgg caatgtaatg
     7201 aacgggtggg taactttctg caaggtgata cgagccgcca accccaacaa tggggacgtc
     7261 gacatcagcc agcgcttgct cgatctgttt gtcgtcgaag tcggcaatga cgccatctcc
     7321 taaccagtcc ttgattttat caatgcgggc gcggaaatct tcttcaatga aaatatccca
     7381 ttccgattgt gacgcctgta aatattcccc tacgccctct actacctgcc ggtcataggc
     7441 tttattggca ttgaacagta atgtgatgcg gtgacgttta gtaaacatgg ttcttttcct
     7501 gctgaatcat gcaaaaactc aaaaccggta atacgttacc ggctttgaga aaatttttat
     7561 caaaatcaag aacggcgttt ggttgcggag tccatccata ctgccagcaa cagaatcgca
     7621 cctttaacga tatactgcca gaaggtcggt acatccatca tactcatgcc gttatccagt
     7681 gaagccatga taaatgcccc cattaccgct ccagccacgc tcccgacacc gcctgccaga
     7741 ctggtgccgc caatcacgca tgctgcaatt gcgtccagct cggcgatatt ccccgcagaa
     7801 ggtgaaccag cgccaagtcg agaactgagg attaatccgg cgatggctac cattaatccg
     7861 ttaatagcga acacggcaag tttggtgcgt tcaacgttaa tgccggagag acgtgctgct
     7921 tccagattgc cgccgatggc ataaatgcgt cgtccaaatg ctgtccgcgt tgccataaac
     7981 attccgccga gtaacagcaa cgtcagcagc agaacaggag tgggaacgcc acggtaatca
     8041 ttcaacagcc agattgcgcc taatacgatg atagcggtta aagcctggcg accgactact
     8101 gcggtagagg ccggagactg caaacccaaa gcctgacggc gcattcttcc gcgccattgc
     8161 cagccaacaa aagccattaa gccaagcgcg ccaatgatga agccggtgct ggcggggaga
     8221 tagctttgcc caatttgtga catcgcggcg ctggtggggg atacagtcgt gccgttggtg
     8281 atgccaatga gtatgccgcg aaatgccaac atgcccgcga gagtgacaat aaatgaaggg
     8341 actttgcggt acgcgaccca ccatccgttc caggcaccga gaagcagtcc cagaaccaac
     8401 gtcacaatga tggtaagtgg caaaggccag cctagccaga cgtcacaaat cgccgcgacg
     8461 ccacctaaca gccccatcat tgagccgacg gaaaggtcga tttcagcaga aattatgacg
     8521 aacaccattc ctaccgcgag gatgccggta attgcggtct ggcgtaacag gttggagacg
     8581 ttacgggcgc ttaagtaggc accatcggtg gtccaggtaa agaacagcat gattgcgatg
     8641 atagctgcaa tcatcacgaa gacctgcaaa ttcagtgctt tcagcccgga gaagccaccg
     8701 gatgtcggta cggccaattt cacttcagac ggattgcttt tcgacatgat gttcgctcct
     8761 caatgcggct tccatcacct gttcctgagt caggttatga tttatcaggt tggcttttag
     8821 tttcccttca tgcatcacca gtacacgatc gctaaggccg agcacttcag gtaattcaga
     8881 agagatgaca ataacggcaa taccctgctg gacgagttgg ttaattaatt tgtagatctc
     8941 gtatttcgcg ccaatatcaa tacccctggt aggttcatca agaatgagaa tgcgcgggtt
     9001 gagtaacaga cagcgggcga ggatcgcttt ttgctgattg ccgccgctca aacgtccaat
     9061 agcaaggtcg ggggacgacg ttttaacttt gagttgctgg attgattcca gaatacattt
     9121 ttgctctgcc gcgtcatcaa gctggctaat gccaccggta aatttattga gtgcggcgag
     9181 ggtaatattt ttaccaaccg ccattaccgg aacgatgccg tcgcgctttc tgtcttcggg
     9241 gagcatcgca atcccctggg cgatggcttg ctgacagtta cgaatatcta cctgtttgcc
     9301 atcaatataa atttttcctt cccattgtcc gggccacaca ccaaacaggc actgaatggt
     9361 ctcggtacgt ccggcaccaa cgagtccggc aatacccagt atttcgccac gtttcaggga
     9421 aaacgagaca tcattaactc gtttaatatg acgattaacc ggatgccatg ccgtcagatg
     9481 ttcaatacgt aatatttcat ctccggtggt atgtggttca ttagggtaaa gcgcggttaa
     9541 ctctcgcccg accatcatgg tgataatatc gtcttcactc attccggcag catcacgcgt
     9601 accaatgtgc tgtccgtcgc gaataacgca aatcgtatcg gaaatcgctt tgacttcgtt
     9661 gagtttgtgc gaaatataaa tacaggcgat accgtgctgt tgtagatcgc gaataatatc
     9721 cagtaaaacc gacgtttcct gctcagttaa tgaggctgtc ggttcatcga gaattaacaa
     9781 gcgcacctgt ttattaagtg ccttggcaat ttcaaccagt tgttgttgcc caagccctaa
     9841 atcgccaacg cgggtatcag gtgaaatgga taaactgacc tgtgcgagca gcttctgaca
     9901 gcgtagcgtc atcaggtcat aatccataat gccattgtgg gttatttcgt tacccaggaa
     9961 gatattttcc agcacggtca attctttcac cagggccaat tcctggtgaa tgatggcgat
    10021 acctttgcgt tcggtatcgc ggatgtggct cgcctgaatc tcttctcccg caaaaataat
    10081 ttcgccttcg taggagccat ggggataaat accacacagc actttcatca gcgttgattt
    10141 accagaccca ttttccccac agagtgagac gatttcgcca gcattcaacc gcaagctgac
    10201 gttatcaatc gccttcacac tgccgaaggt tttggtaatg ttcttcattt caagtagata
    10261 aggcataacg actccaccta agccaattca ttcacgcggc atggagagaa atcacgcccc
    10321 cgctccgcgc tggggcgtaa cgcttacagc tcgctctctt tgtggaatcc gtctttaatt
    10381 accgtatctt tgatgttgtt tttattcaca tcgatcggtg tcaggaggcg ggagggcaca
    10441 tctttcaggc cattattcag tgtggtatct gcttttggtt cctgaccatt gcccaactca
    10501 acggcaattt ctgcggcagt atttgccaac aacgtaatag gtttatacac cgtcatagtt
    10561 tgcgtaccgg cagcaatacg tttaatacct gcgagatccg catcctggcc ggaaattgct
    10621 actttccctg ataaaccttg cgcgcttaat gcctgaattg ccccacctgc ggtggcatcg
    10681 tttgaggcaa ctacagcatc aattttgtta ttattggcgg ttagcgcgtt ttccataatt
    10741 ttcaatgcgt tttccggtaa ccagccatca acccattggt caccaacgac tttaattttt
    10801 ccggaatcaa cgtaaggttt taacactttc atttgtccgg cgcggaacag cttagcgttg
    10861 ttatctaccg gcgagccgcc catcaggaag taattacctt gcggaacaat atcgaccagg
    10921 gcttttgcct gcaattcacc gactttttcg ttatcgaaag aaatataaaa atcgatatcc
    10981 gcatcgttaa tcatacggtc gtaagctaat actttaatgc cttcttgttt ggcttctttt
    11041 acaacgttac ttaatacctg accgttatac ggaataatga caagaacatc gacaccccgg
    11101 tttatcatgt tttcaatctg cgacatttgt gtttcttcat tgccatttgc agactgtaca
    11161 aatactttcg cgccgagaga ttctgccttt ttcacaaaaa tatctcgatc tttttgccag
    11221 cgttcaagac ggagatcatc aatcgccata cctattttga cttctttggc gtgtgccgca
    11281 acgttggtaa gcagaagtga ggtgcaaagg gtgagtagaa tgttctttat tttcatggtg
    11341 tagggccttc tgtagttaga ggacagcttt aataagtaac aatcaccgcg ataaacgtaa
    11401 ccaattttta gcaactaaac aggggaaaac aattacagat ttttatcttt cgattacgat
    11461 ttttggttta tttcttgatt tatgaccgag atcttacttt tgttgcgcaa ttgtacttat
    11521 tgcatttttc tcttcgagga attacccagt ttcatcattc cattttattt tgcgagcgag
    11581 cgcacacttg tgaattatct caatagcagt gtgaaataac ataattgagc aactgaaagg
    11641 gagtgcccaa tattacgaca tcatccatca cccgcggcat tacctgatta tggagttcaa
    11701 tatgcaagcc tattttgacc agctcgatcg cgttcgttat gaaggctcaa aatcctcaaa
    11761 cccgttagca ttccgtcact acaatcccga cgaactggtg ttgggcaagc gtatggaaga
    11821 gcacttgcga tttgccgcct gctactggca caccttctgc tggaacgggg cggatatgtt
    11881 tggtgtgggg gcgtttaatc gtccgtggca gcagcctggt gaggcactgg cgttggcgaa
    11941 gcgtaaagca gatgtcgcat ttgagttttt ccacaagtta catgtgccat tttattgctt
    12001 ccacgatgtg gatgtttccc ctgagggcgc gtcgttaaaa gagtacatca ataattttgc
    12061 gcaaatggtt gatgttctgg caggcaagca agaagagagc ggcgtgaagc tgctgtgggg
    12121 aaccgctaac tgctttacaa accctcgcta tggcgcgggt gcggcgacga acccagatcc
    12181 agaagtcttc agctgggcgg caacgcaagt tgttacagcg atggaagcaa cccataaatt
    12241 gggtggtgaa aactatgtcc tgtggggcgg tcgtgaaggt tacgaaacgc tgttaaatac
    12301 cgatttgcgt caggagcgtg aacaactggg ccgctttatg caaatggtgg ttgagcataa
    12361 acataaaatc ggcttccagg gcacgttgct tatcgaaccg aaaccgcaag aaccgactaa
    12421 acatcaatat gattacgatg ctgcgacagt ctatggcttc ctgaaacagt ttggtctgga
    12481 aaaagagatt aaactgaaca ttgaagctaa ccacgcgacg ctggcaggtc actctttcca
    12541 tcatgaaata gccaccgcca ttgcgcttgg cctgttcggt tctgtcgacg cgaaccgtgg
    12601 cgatgcgcaa ctgggctggg acaccgacca gttcccgaac agtgtggaag agaatgcgct
    12661 ggtgatgtat gaaattctca aagcaggcgg tttcaccacc ggtggtctga acttcgatgc
    12721 caaagtacgt cgtcaaagta ctgataaata tgatctgttt tacggtcata tcggcgcgat
    12781 ggatacgatg gcactggcgc tgaaaattgc agcgcgcatg attgaagatg gcgagctgga
    12841 taaacgcatc gcgcagcgtt attccggctg gaatagcgaa ttgggccagc aaatcctgaa
    12901 aggccaaatg tcactggcag atttagccaa atatgctcag gaacataatt tgtctccggt
    12961 gcatcagagt ggtcgccagg agcaactgga aaatctggta aatcattatc tgttcgacaa
    13021 ataacggcta actgtgcagt ccgttggccc ggttatcggt agcgataccg ggcatttttt
    13081 taaggaacga tagatatgta tatcgggata gatcttggca cctcgggcgt aaaagttatt
    13141 ttgctcaacg agcagggtga ggtggttgct tcgcaaacgg aaaagctgac cgtttcgcgc
    13201 ccgcatccac tctggtcgga acaagacccg gaacagtggt ggcaggcaac tgatcgcgca
    13261 atgaaagctc tgggcgatca gcattctctg caggacgtta aagcattggg tattgccggc
    13321 cagatgcatg gagcaacctt gctggatgct caacaacggg tattgcgacc tgccattttg
    13381 tggaacgacg ggcgctgtgc gcaagagtgc actttgctgg aagcgcgagt tccgcaatcg
    13441 cgggtgatta ccggtaacct gatgatgccc ggatttactg cgcctaaatt gctatggatt
    13501 cagcggcatg agccggagat attccgtcaa atcgacaaag tattattacc gaaagattac
    13561 ttgcgtctgc gtatgacggg ggagtttgcc agcgatatgt ctgacgcagc tggcaccatg
    13621 tggctggatg tcgcaaagcg tgactggagt gacgtcatgc tgcaggcttg ccacttatct
    13681 cgtgaccaga tgcccgcatt atacgaaggc agcgaaatta ctggtgcttt gttacctgaa
    13741 gttgcgaaag cgtggggtat ggcgacggtg ccagttgtcg caggcggtgg cgacaatgca
    13801 gctggtgcag ttggtgtggg aatggttgat gctaatcagg caatgttatc gctggggacg
    13861 tcgggggtct attttgctgt cagcgaaggg tttttaagca agccagaaag cgccgtacac
    13921 agcttttgcc atgcgctacc gcagcgttgg catttaatgt ctgtaatgct gagtgcagcg
    13981 tcgtgtctgg attgggccgc gaaattaacc ggcctgagca atgtcccagc tttaatcgct
    14041 gccgctcaac aggctgatga aagtgccgag ccagtttggt ttctgcctta tctttccggc
    14101 gagcgtacgc cacacaataa tccccaggcg aaggggggtt tctttggttt gactcatcaa
    14161 catggcccca atgaactggc gcgagcagtg ctggaaggcg tgggttatgc gctggcagat
    14221 ggcatggatg tcgtgcatgc ctgcggcatt aaaccgcaaa gtgttacgtt gattggtggc
    14281 ggggcgcgta gtgagtactg gcgtcagatg ctggcggata tcagcggtca gcagctcgat
    14341 taccgtacgg ggggggatgt ggggccagca ctgggcgcag caaggctggc gcagattgcg
    14401 gcgaatccag agaaatcgct cattgagttg ttgccgcagc taccgttaga acagtcgcat
    14461 ctaccagatg cgcagcgtta tgccgcttat cagccacgac gtgaaacatt ccgtcgcctc
    14521 tatcagcaac ttctgccatt aatggcgtaa acgttatccc ctgcctgacc gggtggggga
    14581 taattcacat ctatatatct cagcaattaa ttaatattta gtatgaattt attctgaaaa
    14641 tcatttgtta atggcatttt tcagttttgt ctttcgttgg ttactcgtaa tgtatcgctg
    14701 gtagatatgg agatcgttat gaaaacctca aaaactgtgg caaaactatt atttgttgtc
    14761 ggggcgctgg tttatctggt tgggctatgg atctcatgcc cattgttgag tggaaaaggc
    14821 tattttcttg gcgtgttaat gacagcaact tttggcaact atgcatatct tcgcgcagaa
    14881 aaactcgggc aactggataa tttttttacc catatttgcc agttagttgc gttaatcact
    14941 atcggtctct tgtttatcgg tgttttaaat gcacctatca atgcttatga aatggtgatc
    15001 taccccatcg ccttttttgt ctgcttgttt ggtcaaatgc gtttgtttcg ctcggtatga
    15061 gcaacataaa gctcttacac attcaggaat gaaaggaata ctgtgatgga caacaaaata
    15121 tcaacctatt caccggcctt tagtattgtg tcatggatag ctctcgttgg tggtatcgtt
    15181 acctatctgt tagggctatg gaatgcagag atgcagttaa atgaaaaagg atattatttt
    15241 gccgtactgg tattaggact gttttctgcg gcgtcttatc aaaagaccgt tcgggacaag
    15301 tatgaaggca taccgaccac ttccatttat tatatgacct gcctgactgt ctttattatc
    15361 tctgttgcat tactgatggt aggtctgtgg aatgcgacat tattactcag cgaaaaaggt
    15421 ttttatggac tggctttctt cttaagcttg tttggtgcag tagcggtgca gaaaaatatt
    15481 cgtgatgccg gaataaaccc accaaaagaa acacaggtta cctaggaaga atacagcgaa
    15541 taactcacgt aagcccggtc agtccaatgt gaccgggctt ttacttaact cactaatctg
    15601 tttctgtcga ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa cgttgcgcaa
    15661 aagatccaaa taatatccag tattggccaa tttttaagct caattccccg ggtgcgcagc
    15721 gcatggataa tcaaggcgtg gaatccgtat atacccaacg aatggcggga gattaagcca
    15781 agtccgcgaa tggtacgcgt atccagcgtg tttttaacca gagtcaatag cgcgattgcg
    15841 cagataaaaa ccatcggccc acagtaaaga taccaggtat cggcaaaatt tccgcgccac
    15901 tgcaattcat ataatgtccc gcgagagata ataaaaactc ccgtcgcaaa cagtgcggcg
    15961 ctcacccacg acagtgcttt atgctgtgtg tccatcatcc ctatagcgcg gcccaacatg
    16021 ccatacagaa tgtagtaaaa agtatcgcca ttgatatata agttaattgg cagccattca
    16081 aaaccgtcga ttttctgcgg cacggtgttt gggttagcga tgataccaat caccaccatt
    16141 agcaccagca acattttttc gccgacgttc ttcacctgaa tcagcggtga aaccagataa
    16201 atcaccgcaa tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg cagcaggttt
    16261 ttcagcgcta actccacatt aatggaggta aacagtgcaa tgtagagcag cgcgattgcg
    16321 ctataaaaaa acagacataa gccgatacgc aagaaatggc gcggctgggc gctgcgttcg
    16381 ccaaaaaaga gatagccgga aatcatgaaa aatagcggca cgctgacacg agaggcagag
    16441 ttcagaacat tggcgatatc ccaggtgacg gggctaacac tatgagcatt ggtcacatac
    16501 caggtagtgg tgtgaatcat caccaccatt aaacacgcta tccctcgcag gttatcaatc
    16561 cagtaaattt tgggctgcat ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa
    16621 atcattcagt tggcgcactg gaataatctg agttttaaca ctacagctta tagaggctta
    16681 aggaaattcg taagatatca gccactatac cgatataaat aataagactc accagcaaac
    16741 cagacggtaa tttaatgatg atgaactctt tctttccggc aatggcgctt atggtgctag
    16801 tgggttgttc tacaccgtca cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc
    16861 gttcgttgaa tatggaagcg ttatgcaagg atcaggcggc aaaacgttat aacaccggcg
    16921 agcaaaaaat cgacgtcacc gccttcgaac agttccaggg aagctatgaa atgcgcggtt
    16981 ataccttccg taaagagcag tttgtctgtt cttttgacgc agatggccat tttttgcatc
    17041 tttccatgcg ttaagccctg ctttttcccg tttcgtactg tatatcttcc atccagcggg
    17101 tatactgatc ccttccttta aatccacacg tatccagcac gaaataatat gcaaaagttt
    17161 gataccagga ccttccaggg cttgatcctg accttacagg attactgggc tcgccagggc
    17221 tgcaccattg ttcaaccatt ggacatggaa gtcggcgcgg gaacctctca cccaatgacc
    17281 tgtctgcgcg cgctggggcc agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc
    17341 ccgaccgatg gtcgctacgg cgaaaacccc aaccgtttac agcactacta tcagttccag
    17401 gtggtcatta agccatcgcc ggacaatatt caggagctgt accttggttc tctgaaagag
    17461 ctgggcatgg acccgactat ccacgatatc cgtttcgtgg aagataactg ggaaaacccg
    17521 acgctgggtg cctggggact gggctgggaa gtgtggctaa acggcatgga agtgacgcag
    17581 ttcacttact tccagcaggt tggtggtctg gagtgtaaac cggttaccgg cgagatcacc
    17641 tacggtctgg aacgtctggc catgtacatt cagggcgtag acagcgttta cgacctggtc
    17701 tggagcgacg gcccgctggg taaaaccacc tacggcgacg tgttccatca gaacgaagtg
    17761 gagcagtcca cttacaactt cgaatacgcg gatgtggact tcctgttcac ctgctttgag
    17821 cagtacgaga aagaagcgca gcagctgctg gcgctggaaa atccgctgcc gctgccagct
    17881 tacgagcgta ttctgaaagc cgcccacagc ttcaacctgc tggatgcgcg taaagccatc
    17941 tccgtcaccg agcgtcagcg ctacattctg cgcattcgca ccctgaccaa agcagtggca
    18001 gaagcatatt acgcttcccg tgaagccctc ggcttcccga tgtgcaacaa agataagtaa
    18061 gaggcggcta tgtctgagaa aacttttctg gtggaaatcg gcactgaaga gctgccacca
    18121 aaagcactgc gcagcctggc agagtccttt gctgcgaact ttactgcgga gctggataac
    18181 gcaggcctcg cacacggcac cgttcaatgg tttgccgctc cgcgtcgtct ggcgctgaaa
    18241 gtagctaacc tggcggaagc gcaaccggat cgtgaaatcg aaaaacgcgg cccggcgatt
    18301 gcccaagcgt tcgacgctga aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt
    18361 tgcggtatta ccgttgacca ggctgagcgt ctgactaccg ataaaggcga atggctgctg
    18421 tatcgcgccc atgtgaaggg cgaaagcact gaagcactgc tgccgaatat ggttgcgact
    18481 tcgctggcga aactgccgat tccgaaactg atgcgctggg gggcaagcga cgtgcacttc
    18541 gtgcgtccgg tgcacaccgt gaccctgctg ctgggcgaca aagtcattcc ggcaaccatt
    18601 ctgggcattc agtccgatcg cgtgattcgc ggccaccgct ttatgggcga gccggaattc
    18661 accatcgata acgccgatca gtatccggaa attctgcgtg agcgcgggaa agtcatcgcc
    18721 gattacgaag aacgtaaggc gaagattaaa gccgatgccg aagaagcagc gcgtaagatt
    18781 ggcggtaacg ctgacttaag cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg
    18841 ccggtcgttc taaccgcgaa atttgaagag aaattcctcg cggtgccgtc tgaagcactg
    18901 gtttacacca tgaaaggtga ccagaagtac ttcccggtgt atgcgaacga cggcaaactg
    18961 ctgccgaact ttatcttcgt tgccaacatc gaatcgaaag atccgcagca aattatctct
    19021 ggtaacgaga aagtcgttcg tccacgtctg gctgatgccg agttcttctt caacaccgac
    19081 cgtaaaaaac gtctggaaga taacctgccg cgcctgcaaa ccgtgctgtt ccagcaacag
    19141 ctgggtacgc tgcgtgacaa aactgaccgc atccaggcgc tggctggctg gattgctgaa
    19201 cagattggcg ctgacgttaa ccacgcaacc cgtgcgggcc tgctgtctaa gtgtgacctg
    19261 atgaccaaca tggtcttcga gttcaccgac acccagggcg ttatggggat gcactacgcg
    19321 cgtcacgatg gcgaagcgga agacgtagcc gtcgcgctga atgagcagta tcagccgcgc
    19381 tttgccggtg atgacctgcc gtccaacccg gtagcttgtg cgctggcgat tgctgataag
    19441 atggatactc tggcgggtat ctttggtatc ggtcagcatc cgaaaggcga caaagacccg
    19501 tttgcgctgc gtcgtgccgc acttggcgtg ctgcgaatta tcgttgagaa gaacctcaac
    19561 cttgatctgc aaacgctgac cgaagaagcg gtgcgtctgt atggcgataa gctgactaat
    19621 gccaacgtcg ttgatgatgt tatcgacttt atgctcggtc gcttccgcgc ctggtatcag
    19681 gacgaaggtt ataccgttga caccatccag gcggtactgg cgcgtcgtcc gactcgtccg
    19741 gctgatttcg atgcccgcat gaaagcggta tcgcacttcc gtaccctgga agcagctgca
    19801 gcactggcgg cggcgaacaa acgtgtatct aacatcctgg cgaaatctga cgaagtgctg
    19861 agcgatcgcg tgaatgcctc tactctgaaa gagccggaag aaattaaact ggcgatgcag
    19921 gttgtggtgc tacgtgacaa gctggagccg tactttgcgg aaggtcgtta ccaggatgcg
    19981 ctggtcgaac tggcagagct gcgtgaaccg gtcgatgcct tcttcgataa agtgatggtc
    20041 atggttgatg acaaagaatt gcgtatcaac cgtctgacca tgctggagaa actgcgcgaa
    20101 ctgttcctgc gcgttgcgga tatttcgctg ttgcagtaat aacgccgtta ttaaaaagcc
    20161 tgccatctgg caggcttttt ttatcgctaa ataatacagc aacctttaat aatcttctgc
    20221 tgaataaaga ttatctcata
//
LOCUS       DAZUGK010000074        20151 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZUGK010000074 DAZUGK010000000
VERSION     DAZUGK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.074"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(151..897)
                     /gene="cutC"
                     /locus_tag="ACHY5W_002320"
     CDS             complement(151..897)
                     /gene="cutC"
                     /locus_tag="ACHY5W_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4447209.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAEFGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(911..1477)
                     /gene="yecM"
                     /locus_tag="ACHY5W_002321"
     CDS             complement(911..1477)
                     /gene="yecM"
                     /locus_tag="ACHY5W_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4447210.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1693..3426
                     /gene="argS"
                     /locus_tag="ACHY5W_002322"
     CDS             1693..3426
                     /gene="argS"
                     /locus_tag="ACHY5W_002322"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4447211.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3468..3512)
                     /locus_tag="ACHY5W_002323"
     CDS             complement(3468..3512)
                     /locus_tag="ACHY5W_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447212.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3603..4091
                     /gene="yecT"
                     /locus_tag="ACHY5W_002324"
     CDS             3603..4091
                     /gene="yecT"
                     /locus_tag="ACHY5W_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFS4447213.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4213..4605)
                     /gene="flhe"
                     /locus_tag="ACHY5W_002325"
     CDS             complement(4213..4605)
                     /gene="flhe"
                     /locus_tag="ACHY5W_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFS4447214.1"
                     /translation="MRTLLAMLLFPVMAQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     SRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIPTAEPL
                     RFIWEVPGGGRLIPPLRVQRNEVIVNYR"
     gene            complement(4605..6683)
                     /gene="flhA"
                     /locus_tag="ACHY5W_002326"
     CDS             complement(4605..6683)
                     /gene="flhA"
                     /locus_tag="ACHY5W_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS4447215.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATIDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQHLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6676..7824)
                     /gene="flhB"
                     /locus_tag="ACHY5W_002327"
     CDS             complement(6676..7824)
                     /gene="flhB"
                     /locus_tag="ACHY5W_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFS4447216.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8026..8670)
                     /gene="cheZ"
                     /locus_tag="ACHY5W_002328"
     CDS             complement(8026..8670)
                     /gene="cheZ"
                     /locus_tag="ACHY5W_002328"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFS4447217.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8681..9070)
                     /gene="cheY"
                     /locus_tag="ACHY5W_002329"
     CDS             complement(8681..9070)
                     /gene="cheY"
                     /locus_tag="ACHY5W_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFS4447218.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGALSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9085..10134)
                     /gene="cheB"
                     /locus_tag="ACHY5W_002330"
     CDS             complement(9085..10134)
                     /gene="cheB"
                     /locus_tag="ACHY5W_002330"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFS4447219.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10137..10997)
                     /gene="cheR"
                     /locus_tag="ACHY5W_002331"
     CDS             complement(10137..10997)
                     /gene="cheR"
                     /locus_tag="ACHY5W_002331"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFS4447220.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11016..12617)
                     /gene="tap"
                     /locus_tag="ACHY5W_002332"
                     /pseudo
     CDS             complement(11016..12617)
                     /gene="tap"
                     /locus_tag="ACHY5W_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
     gene            complement(12663..14324)
                     /gene="tar"
                     /locus_tag="ACHY5W_002333"
     CDS             complement(12663..14324)
                     /gene="tar"
                     /locus_tag="ACHY5W_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFS4447221.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14469..14972)
                     /gene="cheW"
                     /locus_tag="ACHY5W_002334"
     CDS             complement(14469..14972)
                     /gene="cheW"
                     /locus_tag="ACHY5W_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFS4447222.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14993..16957)
                     /gene="cheA"
                     /locus_tag="ACHY5W_002335"
     CDS             complement(14993..16957)
                     /gene="cheA"
                     /locus_tag="ACHY5W_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFS4447223.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16962..17888)
                     /gene="motB"
                     /locus_tag="ACHY5W_002336"
     CDS             complement(16962..17888)
                     /gene="motB"
                     /locus_tag="ACHY5W_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFS4447224.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17885..18772)
                     /gene="motA"
                     /locus_tag="ACHY5W_002337"
     CDS             complement(17885..18772)
                     /gene="motA"
                     /locus_tag="ACHY5W_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS4447225.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18899..19477)
                     /gene="flhC"
                     /locus_tag="ACHY5W_002338"
     CDS             complement(18899..19477)
                     /gene="flhC"
                     /locus_tag="ACHY5W_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFS4447226.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19480..19830)
                     /gene="flhD"
                     /locus_tag="ACHY5W_002339"
     CDS             complement(19480..19830)
                     /gene="flhD"
                     /locus_tag="ACHY5W_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFS4447227.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4825 a         5563 c         4914 g         4849 t
ORIGIN      
        1 acaacgctat gcgcactgtc acccgctcaa cacatcacgg cagtcgttcg ttttttatac
       61 tgttcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
      121 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
      181 tttcatttca gcaaccgccg ccccgtctac gacataacgc gaatactcgt ccgcgtgtgc
      241 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
      301 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
      361 gactcctgct ccggccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
      421 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agcacccggg caatgccgaa
      481 ttccgcaaga ttattgagtg tatttagagg gttagcgcac atatcaaagg cgcgatgaaa
      541 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
      601 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
      661 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
      721 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
      781 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
      841 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
      901 tactccttaa ttacgccgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
      961 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     1021 caccttttgg cgaactggtt ttcactgaga tccccggtag gctgagtcct tcatcagaaa
     1081 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc tggcagaacg atttcaatat
     1141 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acaatagaaa
     1201 actgccagtg agcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     1261 tgatcatatt ttctgacaaa agctcgccgc actgttcaaa cccgcgacgc cagcgttcag
     1321 cggtggcgtt ttgatggcaa cgcaaggaaa tgtgatcggc agtcaacgga gtgatattca
     1381 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     1441 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     1501 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     1561 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     1621 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     1681 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     1741 gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc agtcagcaaa agttcagttc
     1801 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     1861 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     1921 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     1981 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     2041 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     2101 attattggtg acgcagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     2161 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     2221 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     2281 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     2341 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     2401 acgcagaacc agatcaccta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     2461 ggtgaaagcc tctacaaccc aatgctgccg ggtatcgtgg cggacctgaa agccaaaggt
     2521 ctggcagtag aaagtgaagg cgcgaccgtc gtattccttg atgagttcaa aaacaaggaa
     2581 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     2641 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     2701 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     2761 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     2821 ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggccgatct gctggatgaa
     2881 gccctggaac gtgcacgccg tctggtggca gaaaagaacc cggatatgcc agccgacgag
     2941 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     3001 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     3061 gcgccataca tgcagtatgc atacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     3121 aacgaagagc aactggctgc agctccggtt atcatccgtg aagatcgtga agcgcaactg
     3181 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     3241 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     3301 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
     3361 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
     3421 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
     3481 ttgccatcta aatgtattct gaaaacggac atgccatcgt tttctcactg ttgagtaaga
     3541 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
     3601 cagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
     3661 atacggttat agtaaatgat catgacattt cagccatcaa agattgttgg caaaaaaatt
     3721 cagatgatga tactgacatt aacgtgatca aatcatgtct gcgacaagaa tacaacctcg
     3781 tcgatgcgca attaaataaa gcttatggtg aagcttatcg ttatatagaa caagtgccac
     3841 gcacaggtgc aaaaaaacct gataccgaac aacttaactt gcttaaaaaa tcacaacgag
     3901 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
     3961 atttaagtga cccttattct gaatcagaat ggctatcatg tatgatcata cagaccaata
     4021 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
     4081 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaataat
     4141 tgcaacaaaa catcgagcct ttttcgctga gttattaaac atgctcgcga gcgcgtaatt
     4201 tttatttgtc cttcagcgat aattcacaat cacttcatta cgttgtactc ttagcggcgg
     4261 aattaaccgc ccaccgccag gcacttccca gataaagcgc aacggttctg ccgtcggtat
     4321 accggaaaag gccacggtgg ttccgctctg cccatctaat tcgacacaac gagattgtga
     4381 acacaagcga acccgcagtc ctgctggtgt cgggccgata agctgataac gccacgccac
     4441 cagcgtcatc aatcctgaag caggttgtcg cgaagaaaga ggcgcagaag acatcgactc
     4501 accgcgatga tttagcgtaa ttcctacact acttgcctgc cacatcccct ccccggcggc
     4561 ttgcgccatc accggaaaca acagcatagc caataaggtt ctcattattt accgccaatc
     4621 gtcgccgtca ttcggatatg tcggttatct gatagttcca gattcgacag caccactaac
     4681 tgcggcaaac tgcggcgcag aaagcgagac aataatggtc gcagcgcgtg gttcaccaac
     4741 aatactggcg gcgcacccaa catctcctga cgggatagcg cttcctgagt ttgcgccagc
     4801 aaacgatccg ccaaccctgg ctccaatccg cccccgccct gcaacgcctg tagcaacaaa
     4861 cgttccagcg gtgtatcgag gccaataaca tggacttcat ctttgccagg aaaccactgc
     4921 tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg tggatcactt
     4981 tggatgggtg catgttccgc cagcgtttcg agaatggtgc gcatatcgcg aatcggtact
     5041 ttttcatcga ggagattttg cagcacttta tgcagtgtgg tgagcgtgac gacgccagga
     5101 acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccagcag atgttgcgcc
     5161 tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgct
     5221 accaccgtgc tggcctcaac cacggtgtac ccctgaatct gcgcctgttc ttttagcgca
     5281 ctttcaatcc agatagcatt caggccaaat gccggatcga tggtcgcctc accaggtaac
     5341 gtcccggcag cggttccagg attaatcgcc aaccaacgcc ccggataagc atcaccactg
     5401 cctatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagatc catattgtcg
     5461 cgaatgtgca ccactggcgg cagaaatccc atctcctggg caaatttctt gcgaatactg
     5521 cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat cagtcgataa
     5581 cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg
     5641 gcattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5701 cggatccacc aggccagccc cagcaatcca gcagtgaaca gcaaaaatac caggttcggc
     5761 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5821 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5881 acaatgaccc ccgcggcggt agaaatcacc agcgccggga tttgtgccac cagaccgtca
     5941 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     6001 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     6061 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     6121 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccggcgtt aaggtcggcg
     6181 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6241 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6301 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6361 cctgccgccg ctgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6421 agtgccagac gcaacagcgt ggtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6481 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6541 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6601 atcaaaatcg gtccagcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6661 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6721 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6781 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6841 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6901 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6961 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     7021 gttattgaca atgacatccg ctttcggcac atcggccatc atccgacgcc gtgcggcagc
     7081 tcgctgcatc tgacggatcc gtcctttaac atgggggtcg ccttcgcttt gtttgaactc
     7141 atcacgaata tcctgccgcg acattcgcag ctttttcagg tggctgaaga tttggaaaaa
     7201 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagccctac
     7261 caaatccatc gcattaccca tggcggtaat cggagactcg gccattaagc gcatcatctg
     7321 cggccagtga tgccagagaa aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7381 tgctttaagc aactccgcgc cagtctgtgc cgagaacatc cgttttatgc ccggtagcgg
     7441 gttgagtttg gaaaacttcg gctgcaagga ttttccgcta aataccagcc cccccagcat
     7501 aaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggtagca gcgccagcat
     7561 ggcttctctg atcagcagaa taatttgccc gaggatcaga ttcgggtctt tgataatact
     7621 gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca gcgatacacc
     7681 gccaaaccag ataacactaa cgcccaccag caaaatcagc agtgaggtca gttcacggga
     7741 acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggagtgg gggcttctgt
     7801 tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa gcgtaaatga
     7861 tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg gatttagggc
     7921 ttttgctgcc acacatcaag catagtgtgc gtttgtcgga tgcggcgtca tcgccttatc
     7981 agaccgcctg atatgacgtg gtcacgccac atcaggcaat acaaatcaaa atccaagact
     8041 atccaacaaa tcgtccacct gatcctgact ggctaccaca ccggctttgc tggtatcgac
     8101 ctgaggtcca ttaagcaaac tctggttttc acgttttgga cgcgactcct gttccgggat
     8161 gttttccaac agcaccatca gcaactggcg ttcgatctcc tgaatgacat ccatcatccg
     8221 cttaatgacc tgcccggtga gatcctgaaa atcctgcgcc atcatgattt ccagcagttg
     8281 cgcgttagta aagctggtat gcgcgggtac atctgccaga aattgtcgcg tatctgttac
     8341 caattcacgg gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt
     8401 taacgctttt gctgatttct ccatttgatc ctgatgcggt tgtgacgcct caacactgtt
     8461 cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc
     8521 atcggggatg gcttccgccg cttcggcaat ggcctgatcc agtcccagtt cccgcaaact
     8581 gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc
     8641 gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc tcacatgccc agtttctcaa
     8701 agattttgtt gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc acatagccac
     8761 tggcccccgc ttgcgccgca gcaatgatgt tctctttctt cgcttctgca gtcaccatta
     8821 acactggcaa tgccgacaac gcgccatctg cacgaatcgt tttcagcaac tccaggccat
     8881 ccatattggg catgttccag tcggagataa caaatccata accgcctgcc tgcaacttat
     8941 tgagagcgtc gacgccatct tccgcttcct caacattatt gaatcccagc tctttcagca
     9001 ggttacgcac tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat ttaagttctt
     9061 tatccgccat ttcacactcc tgatttaaat acgtatcgcc tgtccggcac taatttttgc
     9121 caacatttgt tggcttacct ggctaagatc gaccacttcg cagacaccac ccatattgat
     9181 ggcctcgcgc ggcatgccga acaccacgca acttgcttcg ttttgcgcaa gggtccatgc
     9241 ccccgcctga cgcatcgcca acattcctgc cgcgccgtcg ttgcccatac cggtcaggat
     9301 caccccaacc gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca catctaccga
     9361 aggccgatga cggttaaccg ccgggccatc gtgaattttg atttggtaat ttgcgccact
     9421 acgcgccagc tccatatgcc gatcgcccgg cgctatataa gcatgtcctg gcaagacacg
     9481 ctctccgtct tcggcttctt taaccccaat ctggcaaagc ttattaagtc tgtcggcaaa
     9541 agagcgggtg aaacctggcg gcatatgttg ggtaattaac agtgccgggc tggaaagcgg
     9601 caatggttgc agtacgtgac gaattgcctc agttcccccc gttgaagcac caatcgcaat
     9661 cagtttttca gaactcaaca acggccccgc cttcagcgtt gtcggtgccg acaatggctt
     9721 atgtgctgca aggctcgcct ttgctgctgt acgcaccttt tcagcaatca tttcgctata
     9781 cgccagcatt ccttcgcgaa tacccagttg cggtttggtg acaaaatcta tcgcccccag
     9841 ctccagcgcg cgcagcgtga cttcagaccc tttgccggtc agggaagaaa ccatcactac
     9901 gggcattgga cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat
     9961 ttcaacatcc agcgtcaata catcgggatt gaatttctta atcaaatcac gcgcaaccag
    10021 cggatcaggg gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat
    10081 gatctggcgc atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa
    10141 tccttactta gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta
    10201 aagttttcag agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg aacaaagcgg
    10261 cgcaaaatct cctgctgggt agtttgatcg aagtagatca tgacgttacg acagaagatc
    10321 gcatcaaacg gccccggcac ggtgtactgt ttcgccagta aattcagcgg ggcaaaatca
    10381 acatagttcg ccagctcctg acgcacgcgt accagccctt catgcggtcc cgtccctcgc
    10441 atgaaatacc gttgcagttg ctgcggcgtc aggtttttca actcttcatg gcgatagata
    10501 ccgcttctgg ctttttccag cacttcggtg tcgatatcac tggcaaacac tttccagcgt
    10561 ccgggcgcgg tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg ctcttcgccg
    10621 gtcgaagccg ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc gtgatccgcg
    10681 agcagaggga aatgatgtgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg
    10741 ataaacgcct gccactcacc gctgtgctga ttagactcca gcaagttcag ataatgaccg
    10801 aaatccgtca gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt
    10861 ttatggtcag ccagaacgat cccggctcgt tgatagatca attgacttat ccgccgaaaa
    10921 tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca ataaagacgt ttgcccacag
    10981 ggcagagatg aagtcatagc gccttctcaa tcacttcagg ataccactgg cgcaatttgt
    11041 aactgcgccg actcatgtcg tgctacttca tgttcttcaa gggtaaatac cgccacacgc
    11101 gacgaaagat ggtcggcctg attcgccagt tgttccgttg ccaccgccgc ttcttctacc
    11161 agcgaggcgt tctgctgagt cacctgatcc atctggctga cagcctgtgc aacctgctca
    11221 atcccccgcc gttgttcttc cgacgccgaa gcaatttctc ccataatgtc gttcacgcgg
    11281 gtcaccgaac tgacaatatc ggtcatggtc gcggcggcgt tattcaccag tttcgaaccc
    11341 tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc tgcctgcgcg
    11401 ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg cccctgctct
    11461 ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg gaaagcaatt
    11521 ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat ctcctgcatg
    11581 gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc ggcatttttt
    11641 gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc ggttaattgc
    11701 tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg acttgagaga
    11761 tcgttattgc ctgcgacaat ctccgcgata ccaatgtgca tctcctggct tcccttacgc
    11821 acatcactca ccgtcccacg caaagcctgc tgcatggtct tcaggctggc aaaaatggcg
    11881 gtgatctcat tacggccata taccgcaatc ggacgcgcca gattacccgc agcaatgctg
    11941 tcaaaatggc taccgataat ggccagtggt tgaacaatca tcttgcgcgt ccaccacagc
    12001 gcactgctga tgtagatcgc cgcaacaata atcatgctga taaacaccag cgccgaaatc
    12061 tgatagttac gctggctttg cgcactggcg gcttccagca catggttgat ctccagctgc
    12121 caggcctcaa agttaacgtc aaaggcattc tgtgatccct gcaccggcgc agtgaggaaa
    12181 tccgaaagtt gattactttc aagctaggtc gcctgatgtt cgagatcgtt gtgccagcgg
    12241 gcaaaacttt tctccgtctc tttttgcaat gccctgacgt gctcgttgcc cgcagtcatc
    12301 gccataaaac ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc cgtcgtcatc
    12361 aacgttttaa tgtcatccgc cggatagcta agcgctgtca gtgtgcctgc tttgttcagc
    12421 gcggtactgg cctgtaacat taccgcccga gtttgcgcta atgccgcacg ttgctgattg
    12481 ctctgctcga cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa agacatgccg
    12541 ttactgccaa tctgcaagat cccgcagaga atcaaaatta aaaacagcgt ggtcgaaatt
    12601 cgaatacgat taaacatcaa cgctccccat caggcggcaa tgaccgcgtt agtaaatact
    12661 cgtcaaaatg tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg ctgtgccggt
    12721 ggttgctcac tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg gctggctgcc
    12781 agacgaaacg cggaaaccgc ttgcgttaaa cgactagcct gttcttccag cgcagctgcg
    12841 gcggcagctg attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc catttccgaa
    12901 accgccaatg cgacttgatc gatgccacgg ctctgttcat ccgatgccga tgcaatctcg
    12961 cccataatgt cagtcacgcg agtgacagca ttgacgatat tgttcattgt ttccccggcg
    13021 ctttcgacca gcaccgaacc ggtatcaacg cgcgagacgg agtcttcaat gagagctttg
    13081 atctcttttg ccgcctgggc gctacgactg gcaagattac gcacttcgcc cgccaccacg
    13141 gcaaaaccac ggccctgttc acctgcacgc gcggcttcaa ccgcggcatt cagcgcgagg
    13201 atattagtct ggaaggcaat accgtcgata acgctgataa tgtcggcaat tttcttcgaa
    13261 ctatcggcga tctcatgcat cgttttcact acgccatcca ccactttgcc gccgtgctgg
    13321 gcggtgtcgg aggcactttg cgccagttgc gaggcctggc gggcgttatc ggcgttttgc
    13381 ttcactgtcg cggtgagctg ctccatgctg gcggcagttt cttccagcgc ggatgcctgc
    13441 tgttcagtac gggaggaaag atcggtgttg cccgccgcaa tttcacgggt accggcatag
    13501 atggcatctg aaccttcgcg gacatgagtg acggtgtcag tcaaagagcg ttgcatatgt
    13561 gaaacgctct gcgccaggtc gcccatttca ctgcgcccgt caatggtcag ggtattcgcc
    13621 aggttaccac cggcgatttc gcgaatgtga gcaataattt ttgccagcgg tgtaagcaac
    13681 atacggcgaa tgccgtacca cgccaccagc agaatcaata ccaccaccag cgcgataacc
    13741 gccagttgcc actgggcaaa tcggtaatca tctgcgttgt cagtgacgat atcgcgatac
    13801 agtttttcac tgctgagggc gtactgagca aacgcttcgc ccattgcatt ttgcattccc
    13861 tgggttggct gagcgaaata agctccagta ttgccataat caagataatc aatcagttca
    13921 gttaacgctg tgtaatagtt tttatatttt tcatcaatat tacgactggt agcgaccatt
    13981 tcaggtaacg gtgccatgct tttgaatttt ttataatgag tcgcggcctg ggccaatgtt
    14041 ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt gttgattgga ggaatccatc
    14101 atcatccgca ccgctgaacg actcaggtta atgcgcgttt gcagcattaa atcccaggtt
    14161 gacgtcagct cgccctgctg ttcccgtaat tgattggaaa ccacaaagct cttctggcta
    14221 tggtgaagag aagaaaaaaa cagactgccg gaaataagct gtaacagtgc gaataccccc
    14281 agcaccatta ccaacagcgt gactacgcgg atacggttaa tcataaggca ccttcctgaa
    14341 aacaagttga tctcgttatc ggcaaggaag ggggaaactt tattgctgat gccacccgcc
    14401 gcgaaattga aataaaaaac ccgatgcgca gatcatcggg ttcaattcaa ttgaggaaat
    14461 caggagaatt acgccacttc tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc
    14521 agcagttttt cgatgttcac cagaatcaac atccggtcgc ccagtgcgcc cagtccagtg
    14581 agatattctg ttgaaagcgt cacggcaaac tctggtgccg gacgaatttg ctccgccgtc
    14641 aatgaaagca cgtctgaaac gccgtcaacc acgatgccga ccacccgctg tccgagattc
    14701 aggacgataa ctaccgtgtt gtcgttatag tccacatcca cctggctgaa cttaattcgt
    14761 aagtcaacaa tcggcacaat aacgccgcgc agattcgtga cgcctttgat aaacgctggc
    14821 gtgttcgcaa tccgtgttac ctgatcgtag ccacggatct cctgcacttt caggatatca
    14881 ataccgtact cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg
    14941 gccagctttg ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg
    15001 cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca
    15061 gtgccacgct gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt
    15121 tactttcaag gtttttaact acaacctgat gttgaccaat taattgatcc accagcaagg
    15181 cgtagcggcg accgccactt tgtaagatca ccacaattcc ctgggtagct tcggttttcg
    15241 cgcccgcgac gttgaacact ttccacagtt cgacgatggg cagatattca ccacgcactt
    15301 ccagcacccg ctcgccgccg gccagtggat ggagatcggc ttcacggggt tgcagtgatt
    15361 ccataacagc attcagcggc agaatgaaaa cttcatccgc aacgcgtacg gacatgccgt
    15421 cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta ccctgcttcg
    15481 actggatttc gacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga
    15541 cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga gaagcctggt gcaaatatca
    15601 gcatcgcgac ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc gaggccgctt
    15661 ttgccagaat tcgctcacgg tttagccccg ccccatcgtc ggtcacttca atgcaaatgt
    15721 tgccgccctg atgttcggca gacagaatta aatttccgac gctgttttta cctgcggcga
    15781 gccgtttttc tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg
    15841 ggtcgataat gcgttctatc aggctcttgt caagctcggt ggaactgccc accagcgtca
    15901 gttctacctg cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa
    15961 agacatattc catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt
    16021 tacgttgtaa ctgccccatg ctggtaatca aatcaccatg attaaccggg tccagttcgc
    16081 tggaacgctg ggcaagcatg gactgggtga taaccagctc gccgacgagg ttaattaatt
    16141 gatcaacctt ttctaccgct acacggatgc tggtggattc actgctgcgc gtcgtttttt
    16201 cccgctccac gcgaccggtt ggcgcttgtt cggctgccag tttaagcact ggtggggtgg
    16261 atatttttgg cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac
    16321 agagtaccgc tgtgatgtca tcttcggcga tatcgcccgg taatattgcc gagagagaat
    16381 ccgccccttt caccacgtca gttaacgttg tcagatgccc cagctcttct tccagcaggt
    16441 cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg cggcaactga ctgcgactct
    16501 gctcatcttg cggttcactt ttggcaacca cacttaatcg ggtcactgcg gatggcgttt
    16561 cgcctttcgc ttctaatgcc agttgacgca aggcctggca gatataatcg aagctggcgg
    16621 catccggctc ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg tccttcgttt
    16681 ccaaaaacag attgataatg tcggtgttga gttgcatctc acctcgtctg gcttcatcga
    16741 gcaggttttc catcagatgc gtggtttcct gcaaaacgct gaagccaaaa gttcctgccc
    16801 ctcctttgat cgagtgggca gcccgaaaga tggcattcaa ttgttcggca tctggcgctt
    16861 ccggctgcaa aaccagcaga tgctgctcca tatcagccaa cagttcgtcc gcttcatcaa
    16921 aaaatgtctg ataaaaatcg cttatatcca tgctcacgct gtcacctcgg ttcggctgat
    16981 ggcattgtgg gaacactgac ctgtggtgca acctcaggtt tttccagggc gcttactggc
    17041 tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc
    17101 agcaggctga tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa gcgcatcgtt
    17161 gccgccatgc cgacgacacg taacactttg ccgctatcca accctccgac catcagttcg
    17221 cggcgggatg cattggcccg atcggcagaa agctcccagt tgctatatcc tttctcaccg
    17281 ctggcgtagg ggaaatcatc ggtatgaccc gaaaggctaa tacggttggg aataccattc
    17341 agaacgggcg cgatggcgcg cagaatgtcg cgcatatagg gttcgacatc ggcactgccg
    17401 gttttaaaca tcgggcgatt ctggctatcg atgatctgaa tacgtagacc ttcctggacc
    17461 agatcgattt tgagatgtgg acgcaacgcc cgcagtttcg gatcggactc tatcaactgg
    17521 tcgagatcac cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt caactcttcg
    17581 atgttcggct gcttattcac ttccccctgg ctttgggtgt aatcatcgcc accgccggga
    17641 attgggcttt cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc cagtggagtc
    17701 cggaagtact ccgcaatctg aatcaactct tttgggctgg agatggagat cagccacatc
    17761 accagaaaaa aggccatcat cgcggtcata aagtcggcat aagcaatctt ccacgatcca
    17821 tgtgctgccc cgtggctttt ggctttgcgt cgtttgacga caataatcgg atgcgcttga
    17881 ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct
    17941 cttccagttc aataaacgac ggacgttcgc tggaatagag cgttttgcga ccaaactcaa
    18001 cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag tgtgactttg acgcactgca
    18061 tcattttgct ggtttcggcg cttttctgac gtaaaacact cgctaatggg gaaataaatc
    18121 cgtaagccaa taaaatgccg aggaaagtcc ccaccatcgc atgtgcgata agcgccccca
    18181 gctcggcggc aggacgatcg gctgaaccta acgcgtgaac gacccccatt acagccgcga
    18241 caataccaaa cgccggaagt gagtccccga ccagcgccag actgttcgcc gggacttctg
    18301 cctcgctttc gtgcgtctca atctcttcat ccatcagggc ttcgatttcg aaggtgttca
    18361 tgtgaccact gataatcagg cgcagataat cgacgataaa atcaagcatg acgctatccg
    18421 cgaggatgcg tgggtagctg gcgaagatct cgctctcacg gggattttca atatcacgtt
    18481 ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa ccgataaagc agagccagca
    18541 gatccatata cattgctttg gtgtatttgg agcgacgaaa cagcaacggc agcgccttca
    18601 gcgtgccttt aatcgctttg ccattattgc cgacgataaa cgacccaatc cctgcaccgg
    18661 caataatcac cagttcagcg ggttgataga gtgctccaag gcttccaccg gtcatcaaat
    18721 aaccgccgaa aactgtaccg agaacaacca ggtaacctaa taagataagc acgacatcat
    18781 ccttccactg ttgaccatga caggatgttc agtcgtcagg cgttaacgtg cgattggggc
    18841 aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt tgcgccacac cgtatcagtt
    18901 aaacagcctg tactctctgt tcatccagca gttgtgggat aatatcggca ggattctggg
    18961 aaagtttacg tctttttact gcccgggatg gcggttgaca taagctgcag gcaaagctgc
    19021 caacaggctg atgagcgtgg gtaataaaat tgccgccgca gcagttgcag ctggaaagtt
    19081 gcagtaatcc actttcaaca aaccgcacca atgtccaggc acgggttaat gccagcagcg
    19141 gtccttcttc tgcttgcggg cactgttcaa ggtataaacg gtaggctttg atcacagcat
    19201 cgacgccatt acacaaaccg gttttcagta aaaactgcca tgcattacag aacatcgaag
    19261 catgaacgtt ttgttcccag gtcataaacc agtcggttga gaatggcagc atgcctttcg
    19321 gcggtgggct tccgcgcagt tctttataaa gttttatcag gcgtccgcga cttaactgtg
    19381 tttcgctttc cagcatctgc aaacgagcgc ccagggtgat caattccatt gccagctgaa
    19441 tatcccgcgc ttcctgaaca atgctttttt cactcatgat caggcccttt tcttgcgcag
    19501 cgcttcttca ggctgattaa catcattcag caagcgtgtt gagagcatga tgccggtatg
    19561 aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac tgagtaatcg tctggtggct
    19621 gtcaaaacgg aagtgacaaa ccagctgatt ggtttctgcc agcttaacca tttgcggaag
    19681 agtcagtgcc gctaatgttg tcgccatttc ttcatttatg ccgagacgaa acatagcgga
    19741 cgctttgtcc tgaacaatca gacgctgtgc aagtagtaaa tatgacaagt tgatgtcata
    19801 aatgtgtttc agcaactcgg aggtatgcat tattcccacc cagaataacc aactttattt
    19861 ttatgcggtt tcaccgcacc ccgtgatgtc gccgggaagc cccggtaaaa aataattagc
    19921 attagaatag ttgcgataag ctgcaataag cagagccacc tttttggttt aataagtcct
    19981 tacaaataga agggggtctt tacacttatc taagattttt cctaaatcga cgcaactgta
    20041 ctcgtcacta cacgcacata caacggaggg ggggctgcga ttttcaataa tgtgtgatgc
    20101 agatcacaca aaacactcaa ttacttaaca taaatgtata agtcatactt t
//
LOCUS       DAZUGK010000075        20136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZUGK010000075 DAZUGK010000000
VERSION     DAZUGK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.075"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..332)
                     /gene="cbrA"
                     /locus_tag="ACHY5W_002340"
     CDS             complement(<1..332)
                     /gene="cbrA"
                     /locus_tag="ACHY5W_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFS4447228.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSL"
     gene            433..1647
                     /gene="yidR"
                     /locus_tag="ACHY5W_002341"
     CDS             433..1647
                     /gene="yidR"
                     /locus_tag="ACHY5W_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFS4447229.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANLPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(1649..1981)
                     /gene="yidQ"
                     /locus_tag="ACHY5W_002342"
     CDS             complement(1649..1981)
                     /gene="yidQ"
                     /locus_tag="ACHY5W_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS4447230.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    2221..2293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            2287..2700
                     /gene="ibpA"
                     /locus_tag="ACHY5W_002343"
     CDS             2287..2700
                     /gene="ibpA"
                     /locus_tag="ACHY5W_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFS4447231.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2812..3240
                     /gene="ibpB"
                     /locus_tag="ACHY5W_002344"
     CDS             2812..3240
                     /gene="ibpB"
                     /locus_tag="ACHY5W_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFS4447232.1"
                     /translation="MPNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            3436..5097
                     /gene="yidE"
                     /locus_tag="ACHY5W_002345"
     CDS             3436..5097
                     /gene="yidE"
                     /locus_tag="ACHY5W_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFS4447233.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5094..5810)
                     /gene="yidP"
                     /locus_tag="ACHY5W_002346"
     CDS             complement(5094..5810)
                     /gene="yidP"
                     /locus_tag="ACHY5W_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4447234.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            6106..7722
                     /locus_tag="ACHY5W_002347"
     CDS             6106..7722
                     /locus_tag="ACHY5W_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4447235.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGIWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            7722..9044
                     /locus_tag="ACHY5W_002348"
     CDS             7722..9044
                     /locus_tag="ACHY5W_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFS4447236.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIEFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKTLNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPGTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(9041..9934)
                     /gene="yidL"
                     /locus_tag="ACHY5W_002349"
     CDS             complement(9041..9934)
                     /gene="yidL"
                     /locus_tag="ACHY5W_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4447237.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSQEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            10101..11816
                     /gene="yidK"
                     /locus_tag="ACHY5W_002350"
     CDS             10101..11816
                     /gene="yidK"
                     /locus_tag="ACHY5W_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS4447238.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            11813..13306
                     /gene="yidJ"
                     /locus_tag="ACHY5W_002351"
     CDS             11813..13306
                     /gene="yidJ"
                     /locus_tag="ACHY5W_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4447239.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13353..13802)
                     /gene="yidI"
                     /locus_tag="ACHY5W_002352"
     CDS             complement(13353..13802)
                     /gene="yidI"
                     /locus_tag="ACHY5W_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFS4447240.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            13911..14258
                     /gene="yidH"
                     /locus_tag="ACHY5W_002353"
     CDS             13911..14258
                     /gene="yidH"
                     /locus_tag="ACHY5W_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFS4447241.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            14248..14610
                     /gene="yidG"
                     /locus_tag="ACHY5W_002354"
     CDS             14248..14610
                     /gene="yidG"
                     /locus_tag="ACHY5W_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFS4447242.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            14607..15104
                     /gene="yidF"
                     /locus_tag="ACHY5W_002355"
     CDS             14607..15104
                     /gene="yidF"
                     /locus_tag="ACHY5W_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4447243.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15112..16296)
                     /gene="emrD"
                     /locus_tag="ACHY5W_002356"
     CDS             complement(15112..16296)
                     /gene="emrD"
                     /locus_tag="ACHY5W_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFS4447244.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAITTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            16378..16452
                     /gene="ysdE"
                     /locus_tag="ACHY5W_002357"
     CDS             16378..16452
                     /gene="ysdE"
                     /locus_tag="ACHY5W_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFS4447245.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(16576..16665)
                     /gene="tisB"
                     /locus_tag="ACHY5W_002358"
     CDS             complement(16576..16665)
                     /gene="tisB"
                     /locus_tag="ACHY5W_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFS4447246.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            17230..17328
                     /gene="ivbL"
                     /locus_tag="ACHY5W_002359"
     CDS             17230..17328
                     /gene="ivbL"
                     /locus_tag="ACHY5W_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFS4447247.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            17434..19122
                     /gene="ilvB"
                     /locus_tag="ACHY5W_002360"
     CDS             17434..19122
                     /gene="ilvB"
                     /locus_tag="ACHY5W_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFS4447248.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            19126..19416
                     /gene="ilvN"
                     /locus_tag="ACHY5W_002361"
     CDS             19126..19416
                     /gene="ilvN"
                     /locus_tag="ACHY5W_002361"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS4447249.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            19679..20136
                     /locus_tag="ACHY5W_002362"
                     /pseudo
     CDS             19679..20136
                     /locus_tag="ACHY5W_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4837 a         5067 c         5277 g         4955 t
ORIGIN      
        1 atcagcgatt tcatccacaa gtcgaaagca tggcgattaa tattgatata gcttcgctgg
       61 tagttacgcg tgagcgatgc ggcgacgtcg acggttttga cgctgaaaat ctgcgggttg
      121 gcgatcacat cgacaggaag cgtcagtcca tcgcgaataa aagatcgctg cgcgtccggt
      181 gccagcagac cgccgcaggg tttgctgaaa ccttcagtac cacactggtg ctttttatcc
      241 agcgcgatca cctgcatttt gcctgctaac tttcgcgcca acgccgaccc tgccgggccg
      301 aggccaataa tcgccacgtc gaaatgttcc atcttttcac tcacattcat cacgatggca
      361 aaagactatt gtctgaaagt gaagtccttg tgaagtatga tggcgtgaac cgttttgtat
      421 caggaccacg ccatgaaaca gataaccttt gctccccgta atcacctgct caccaatacc
      481 aatacctgga cgcccgacag ccagtggctg gtatttgacg tgcgtccttc tggggcgtcg
      541 tttaccggcg agaccattga gcgtgtgaat atccatactg gagaggtcga ggttatctat
      601 cgcgcgtcac agggcgcaca cgtcggcgtg gtgaccgttc atccgaagtc agaaaaatat
      661 gtcttcattc acggcccgga aaatcctgat gaaacatggt attacgattt ccatcatcgg
      721 cgcggagtca ttgctgaagg cggcaaggtg agcaatctcg atgcaatgga tattactgca
      781 ccgtacaccc caggagcgct gcgcggcggc agccatgtgc atgtctttag cccgaacggt
      841 gaaatggtga gctttaccta taacgaccat gtaatgcatg aacgcagccc ggcgctggat
      901 ttgcgaaacg tcggggttgc tgcaccgttt ggcccggtca acgtacaaaa gcagcatccg
      961 cgtgaataca gcggtagcca ctggtgcgca ctggtgagta aaacaacgcc cacgccgcag
     1021 cctggcagcg atgaaatcaa tcgtgcttat gaagaaggat gggtaggaaa tcacgcgctg
     1081 gcgtttattg gcgatacgct ttcgccaaag ggcgagaaag tgccggagct gtttatcgtt
     1141 gagttaccgc aagatgaagc tggctggaaa gcagcaggtg atgcgccgtt aagtggaacg
     1201 gaaacaaccc tgcccgcgcc accgcgtggc gtcgtgcagc gacgtttaac ctttacccac
     1261 catcgggctt atccggggtt agtcaacgtc ccgcgccact gggtgcgctg taatccgcag
     1321 ggtacgcaaa tcgcgttttt aatgcgagat gataacggca ttgtgcaact gtggcttatc
     1381 tcgccacagg gcggcgagcc gcgccagtta acccataaca aaacggatat tcagtctgca
     1441 tttaactggc atccgtcagg agaatggttg ggctttgtgc tgggtaatcg aattgcttgt
     1501 gcccatgcgc aaagcggcga ggttgagtat ttaaccgaaa accacgccaa tctaccttct
     1561 gcggacgccg tggtcttctc gccggatggt cagtggctgg cgtggatgga aggcggccag
     1621 ctgtggatca ccgaaactga tcgctaaatc agttatcagg cattctggcc ggtggaataa
     1681 ccgcattggt ggcttgcgcg ttggcttcgc ttttttcaac gcgtgagcgc accgagctgt
     1741 ctttgcggaa cacatcccac ggtaataaaa gcgtgtccat cacagcggta aacggcatat
     1801 cgagaatcgc cagcgactta gtgccccagt tggtttcatc atcgccaatc atggtggcgc
     1861 tggcgcgggt gccgggatac gttccttctt tccctccggt atgggacatc acgctggagc
     1921 atcctccgag taatgccatt ccgctgcata tcatgaacgc taacaacaca tttcttatca
     1981 taataaattc atctgttgat cgtgggtgtt ggcctgatga gttatagcga tcccttgctg
     2041 aaaataacat catcattacg tcgctctgtg gcggcaatcg cactttaacg tttcgtgctg
     2101 ccccctcagt ctatgcaata gaccataaac tgcaaaaaaa agtccgctga taaggcttga
     2161 aaagttcatt tccagaccca tttttacatc gtagccgatg aggacgcgcc tgatgggtgt
     2221 tctggctacc tgacctgtcc attgtggaag gtcttacatt ctcgctgatt tcaggagcta
     2281 ttgattatgc gtaactttga tttatccccg ctttaccgtt ctgctattgg atttgaccgt
     2341 ttgtttaacc acttagaaaa caaccagagc cagagtaatg gcggctaccc tccgtataac
     2401 gttgaactgg tagacgaaaa ccattaccgc attgctatcg ctgtggctgg ttttgctgag
     2461 agcgaactgg aaattaccgc ccaggataat ctgctggtgg tgaaaggtgc tcacgccgac
     2521 gaacaaaaag agcgcaccta tctgtaccag ggcatcgctg aacgcaactt tgaacgcaaa
     2581 ttccagttag ctgagaacat tcatgttcgt ggtgctaacc tggtaaatgg tttgctgtat
     2641 atcgatctcg aacgcgtgat tccggaagcg aaaaaaccgc gccgtatcga aatcaactaa
     2701 ttccctaagg ccgcctggcg cggcctgaca tctccatgct cgccgtcagg gagcatatgc
     2761 gaatcttcgg atttgcaggt acttactcgc ttcttagaag gagaaatgac tatgcctaac
     2821 ttcgatttat ccccactgat gcgtcaatgg atcggttttg acaaactggc caacgcactg
     2881 caaaacgccg gtgaaagcca gagcttcccg ccgtacaaca ttgagaaaag cgacgataac
     2941 cactaccgca ttacccttgc gctggcaggt ttccgtcagg aagatttaga gattcaactg
     3001 gaaggtacgc gtctgagtgt aaaaggcacg ccggagcagc caaaagaaga gaaaaaatgg
     3061 ctgcatcaag ggcttatgaa tcagccattt agcctgagct ttacgctggc tgaaaatatg
     3121 gaagtctctg gcgcaacctt cgtaaacggt ttactgcata ttgatttaat tcgtaatgag
     3181 cctgaaccca tcgcagcgca gcgtatcgct atcagcgaac gtcccgcgtt aaatagctaa
     3241 ctagctattc tctttgcccc gccattctgg tggggctttt tttgtctttc ttcttcaaca
     3301 atgcgttaaa tttcgactgt ttaagatatt tcggcacgtt ctttcccgtg ataatgtgcg
     3361 ctataaggca aatctgcttc accgattcag ccataatccg tagcaattaa aatgttatct
     3421 caagagaaat ggacgatgag tgatatagca ttaacggtca gtattctggc tttggtggca
     3481 gtcgtcggtt tgtttatcgg caacgtcaaa tttcgcggca taggattagg tattggcggc
     3541 gtgctgtttg gtgggatcat cgtcggccat tttgtttctc aggcggggat gacattaagt
     3601 agcgatatgc tgcatgttat tcaggaattt ggcctgatcc tgttcgttta tactatcggg
     3661 attcaggtag ggccgggctt ctttgcctca ttgcgcgtct ccggattacg cctcaacctg
     3721 tttgctgttc tgatcgtcat catcggtggt ctggttaccg ccatcctgca taaactgttt
     3781 gatattccac tgccggtagt gctggggatt ttctccggtg cggttaccaa tacgccagcg
     3841 ctgggggcag ggcagcagat cttgcgcgac ctgggtacac caatggaaat ggtcgatcag
     3901 atggggatga gttacgcgat ggcgtatcca ttcggcattt gcgggatttt gttcaccatg
     3961 tggatgttgc gggttatttt ccgcgtcaat gtcgagacag aagcccagca gcacgagtct
     4021 tcacgcacca atggcggcgc gctgatcagg actatcaata ttcgcgttga gaaccctaac
     4081 ctgcatgatt tagccattaa agatgtaccg attctcaacg gcgacaaaat tatctgctcg
     4141 cgtctgaaac gcgaagaaac cctaaaagtt ccttcgccag ataccattat ccaactgggc
     4201 gatttgctgc atctggtggg tcagccagcg gatttacata atgcgcaact ggtgattggt
     4261 caggaggtcg atacttcgct gtccacgaaa ggcactgatt tgcgcgtcga gcgtgtggtg
     4321 gtcaccaatg aaaacgtgct tggtaagcgt attcgcgacc tgcattttaa agaacgctat
     4381 gacgttgtta tctcgcgcct gaaccgtgcc ggggtcgaac tggtcgccag tggcgatatc
     4441 agcctgcagt tcggcgatat cctcaacctg gtggggcgtc cgtccgcaat tgatgccgtt
     4501 gccaatgtgc tggggaatgc gcagcaaaaa ctgcaacagg ttcagatgct gcctgtgttt
     4561 attggcatcg ggcttggcgt attgttaggt tctattcccg tctttgtgcc gggattcccg
     4621 gccgcgttga aactggggct ggcgggcggt ccgctgatta tggcgttgat cctcgggcgt
     4681 atcggcagta tcggcaagct gtactggttt atgccgccaa gtgccaacct cgcgctgcgg
     4741 gagctgggga tcgtgctgtt cctctcggtc gttggtctga aatctggtgg ggattttgtg
     4801 aataccctgg tcaatggcga agggctaagc tggattggtt atggtgccct gatcaccgcc
     4861 gttccgctga ttactgttgg cattctggcg cggatgttag ccaaaatgaa ttacctgacc
     4921 atgtgcggga tgctggcagg ttccatgacc gatcctccgg cgctggcgtt tgctaataat
     4981 cttcatccaa ctagcggtgc ggcggcgctc tcttacgcca ctgtctatcc gttagtgatg
     5041 ttcctgcgca ttatcacccc ccaattactg gcggtgctct tctggagtat cggttaactc
     5101 ttgtccgggt ggagtcgccg taaatggtac tccacctggt attcgctggc atttctgaac
     5161 atcactgaat aattcaaaaa ctccccgctc tcgctatatg acaacgaggt gatccgcagc
     5221 agtggcgtgt gttccgctac cttcatttgc cgcgccagca gtctatcggc gagcaccggc
     5281 gtcaggcttt cataattacc gccaatcaaa atcccgcact cttgttcaat atattcaaac
     5341 ttcgaccctt ccagatgttg cagcgaaagg ttacggaaca gttttaccgg catatagctg
     5401 tcttccagca tcagcggttt cccttccaca aaacgaacgc gacgggagaa gtagatctgc
     5461 tcgttgattt gaatccgtaa ctggctggca atggccggag gcgcaggcat gatttcaaaa
     5521 atcagcacct ggctggtgac tgtttttccc tgccgtttta acacctccac cagcccggtc
     5581 aggctggcgg tttgatgcag tacatctttg cgcaccaggt aagtgccgct gccgtggcgg
     5641 cggaccacca acccccacgc taccagcagg tcaatcgctt tacggatggt catccgcgat
     5701 accgcaaatt cttccgccaa ctttttttca ccgggaagaa ggctgtttag cgtgaaatct
     5761 gcggaattaa gtcgaattct taaccgctcc gcaatgcttt tgtagatcat ctctagatct
     5821 cttttttgcc tgcaagcccg ggtaatgcgc ccacaagttc tgcttaatcg attgaaaaaa
     5881 catctttttt ttaaagatgt gttcgatgct gtgtaccagt gctcacagat gtctactttt
     5941 tcgcgaaaac gtagatctct accgcccaac gaaaagcatg aaagcgatca cgaatcccat
     6001 tgggtcgtca tgttctgccg atcgcatctt cctatcctcg ctccaggcct gccgcataac
     6061 caatcaggct tcctacttac agaattgaga aaagaggatg tggaaatgct cagtcaaatt
     6121 caacgctttg gcggcgcgat gttcacgcca gtgctgctgt ttcccttcgc cgggattgtg
     6181 gtgggtcttg ccatcttgct gcaaaacccg atgtttgtcg gggaatcact gaccgatccg
     6241 aacagtttat tcgcgcaaat cgtacacatt attgaagagg gcggttggac ggtattccgt
     6301 aatatgccgc tgatttttgc tgtcggttta cccattggcc ttgctaagca agcgcaggga
     6361 cgtgcttgtc tggcggtgat ggtgagtttc ctgacctgga actatttcat caatgcgatg
     6421 ggaatgacct ggggaagcta cttcggcgtc gatttcactc aggacgcggt ggcaggtagc
     6481 ggtctgacaa tgatggctgg gattaaaacc ctcgatacca gcattatcgg cgcaattatc
     6541 atttccggca ttgtgacggc gctgcataac cgtctgttcg ataaaaaact gccggttttt
     6601 ctcggcattt tccaggggac gtcttatgtg gtgattatcg ccttcctggt gatgatcccc
     6661 tgtgcctggc tgacattgct cggctggcca aaagtacaaa tggggattga atctctgcaa
     6721 gcgttcctgc gttcggcggg tgcgcttggg atctgggttt acaccttcct cgaacgtatt
     6781 ctgatcccaa ccggtttaca ccacttcatc tacggaccgt ttatctttgg tccggcagct
     6841 gttgaaggcg gcattcagat gtactgggcg cagcatctgc aagagttcag tttgagcgcc
     6901 gagccgctga aatcgttgtt cccggaaggc ggttttgccc tgcacggtaa ctcaaaaatc
     6961 tttggtgccg tgggcatttc tttagcgatg tacttcactg ccgcaccgga aaatcgggta
     7021 aaagtggcgg gcttgctgat tcccgcaacc ttaaccgcca tgctggtggg aattaccgaa
     7081 ccgctggaat ttaccttcct gttcatttca ccgttgctgt ttgcggtaca cgccgtgctg
     7141 gcggcctcaa tgtcgaccgt aatgtatctc tttggtgtgg tgggcaacat gggcggaggt
     7201 ctgattgacc aggttttacc gcaaaactgg atcccgatgt tcagcaacca cgcggatatg
     7261 atgctgaccc aaatcgccat tgggttgtgc tttaccctgc tgtacttcgt ggttttccgc
     7321 accctgattc tgcaattcaa catgtgcacg ccgggacgtg aagatgcgga agtgaaactc
     7381 tactcaaaag ccgaatacaa agcctcgcga ggccaaacca ccgcggcaga gccaaaaaaa
     7441 gagctggatc aggctgccgg tatcctgcaa gccctgggcg gggtcggcaa tatctccagc
     7501 attaacaatt gcgcgacgcg tttacgtatt gcactgcatg acatgtcaca aacgttggat
     7561 gacgaagtct ttaaaaagct gggagcgcac ggcgtcttcc gtagtggcga tgccattcag
     7621 gtgatcattg gtctgcatgt atcccagctg cgtgaacagc tcgatagctt aattaattct
     7681 catcaatcag cagaaaatgt tgccattacg gaggcagtat aatgaccaaa ttctcagtgg
     7741 ttgtcgctgg cggtggaagc acctttacgc caggcatcgt gttgatgctc ctggcgaatc
     7801 aggaccgttt cccgcttcgt gcactgaaat tttatgataa cgatggtgcg cggcaggaag
     7861 tgattgccga agcctgtaaa gtcatcctta aagagaaagc gccggacatt gagtttagtt
     7921 acaccaccga tcctgaagtg gcattcagcg acgttgattt tgtcatggcg cacatccgcg
     7981 tcggcaaata cccgatgcgc gaactggatg aaaaaatccc gctgcgccac ggcgttgttg
     8041 gtcaggaaac ttgcggaccc ggcggaatag cgtacggcat gcgttccatt ggcggcgtcc
     8101 tggaactggt ggattatatg gaaaaatatt caccaaatgc ctggatgctc aactactcca
     8161 acccggcagc cattgtcgca gaagccacgc gtcgtctgcg cccgaatgcg aaaaccctca
     8221 acatctgtga catgccaatc ggtattgaaa gccggatggc gcaaattgtt gggctgcaag
     8281 atcgcaaaca gatgcgcgtg cgctactacg gcctgaacca ctttggctgg tggacatcaa
     8341 ttgaagattt gcagggcaac gacctgatgc cccagctgcg gcaatatgtc tctaagcacg
     8401 gttatgttcc accgcagcaa gatacacata ctgaagcgag ctggaacgac acctatgcaa
     8461 aagcgcggga tgtccaggca ctggacccgg gtacattacc aaacacctat ctgaaatatt
     8521 atctcttccc ggattatgtt gttcagcatt ccaaccctga acatacccgc gcgaatgagg
     8581 tgatggaaca tcgcgagaaa caggttttcg atgcttgccg cgccattacg gcggcaggaa
     8641 attcagcggc gggcaagctg gaaattgacg aacatgcgtc atacatcgtc gatctggcgg
     8701 cggcaattgc cttcaacact caggagcgga tgttgctgat tgtgcctaac aacggggcaa
     8761 ttcataactt tgatgatgaa gcgatggtcg agatcccgtg tctggtcggg cacaacggac
     8821 cagaaccact ggtggtcggc gatatcccgc agtttcagaa agggttaatg agtcagcaag
     8881 tggcggtgga aaaactggtc gtggacgcct gggaacagcg ttcatatcag cacctgtggc
     8941 aggcgattac gttatcgaaa acggtaccga gcgcctcggt cgccaaagct attctggatg
     9001 agttgctgga ggccaacaaa gcgtactggc cagagttacg ttaattgtgt ggactggcat
     9061 ccgttgggtg ctggtccgta ttctttagca cggctgacgg cgcataacca aatttatgtt
     9121 taaaggcttt gctgaaatga aaggagtcga agaagttgag cgtatcagcg acttcgccaa
     9181 ccgagtttcc ttgttgcttt aatagcgata gcgccagatc cagacgcgca tcgaggtaat
     9241 attctttcgg cgtcttgccg gtatagcgta aaaacaggcg acgcaaccag gcttcgctac
     9301 aggggatcgt ggcagccata tcagctacgc tccagcgttg ttgcagactg gcatgtaaag
     9361 tggcaattaa tttttcaatt tgccgccgtt gtggatcttt tttaccgtct gcgtacataa
     9421 gacaaatcca ctgataaata attttcgtca gaaaagcgac tgccagatta ttttttattg
     9481 cttcttgtga agttattaac tcagcaactt cggtgagttc ctgattataa atttcgccat
     9541 tataaataac gctttgctga ccaacgggaa tatccatcat actggtgggg gtaaattcca
     9601 tccagtactg ttcccagact aaaccttcac agtgataaga gtgaatatcc attggcttta
     9661 aaaatataat acagttgcca tgcagagtta tttgttcgcc attctttaaa agaattttcc
     9721 cacatccttg tacggtatac accgcaaccc atgagttggc gataagcggt ttcttcttgt
     9781 cccgtggcca gatcacccgg taatgctcgt cggcgtaggt atgccagagc gagatcagag
     9841 aaattccgct ggagatcaca ttttcgttaa tcaataaagg aatattgtat ggagtttcgt
     9901 tttttacatc tgaagtttgc aattttccat tcattttaat atccctccgg cgaattatgc
     9961 tcacaacctg aacttaacag aaaacaatcc gttcagggat acgacgtttg tattttaaga
    10021 atctgactgc ctgacccgac gcattttacc tctccctata ttcatgcgtc cgggacataa
    10081 acataaataa gggctatgag atgaattcgt tacaaatctt gagttttgtc ggttttacgc
    10141 tgctggtggc gatcatcacc tggtggaagg ttcgcaaaac agataccgga tcgcaacaag
    10201 gctattttct tgccggacgt tcactaaaag cgccggttat tgccgcttcg ttaatgttaa
    10261 ccaacctttc cacggaacaa ctggttggtc tttccgggca ggcctacaaa agcggcatgt
    10321 cggtgatggg ctgggaagtg acttcagcgg tgacgctgat cttcctcgcg ctaatctttt
    10381 taccgcgcta tctgaagcgc ggcattgcca ccatccccga ttttctggag gaacgttatg
    10441 ataaaacgac gcgtattatc atcgacttct gcttcctcat tgccaccggc gtctgctttc
    10501 tgccgattgt tctctactcc ggcgcgttgg cgctcaacag cctgtttcac gtcggggaat
    10561 cgctacagat ttctcacggt gcggctatct ggctactggt aattttgctt ggtctggcgg
    10621 gaattttgta tgcggtgatc ggcggactgc gcgcaatggc agtggcggac tccatcaacg
    10681 gtattgggct ggttatcggc gggttgatgg tgccggtatt tggcttgatc gcgatgggca
    10741 agggcagctt tatgcagggc attgagcaaa ttaccaccgt tcacgccgag aaattaaact
    10801 cagtcggtgg cccgaccgat cccttgccga ttggcgcggc atttaccggt ttaattctgg
    10861 tgaacacctt ttactggtgt acaaatcagg gcatcgtgca acgcacgctg gcgtcaaaaa
    10921 gcctggcgga agggcaaaag ggggcgctgt taacggcggt gctgaaaatg ctcgacccgc
    10981 tggtactggt gctgccaggg ttgattgcgt ttcatctgta tcaggatcta ccgaaagccg
    11041 acatggccta cccgacgctg gtcaataacg ttctgccagt gccactggtg ggtttcttcg
    11101 gcgcggtgtt atttggtgca gtgatcagta ccttcaacgg ctttctgaat agcgccagta
    11161 cgttattcag tatgggtatt taccgtcgca tcattaacca gaatgccgag ccgcagcagc
    11221 tggtcaccgt cgggcgcaaa tttggtttct ttatcgctat cgtttcggtg ctggtagcgc
    11281 cgtggatcgc caacgcgccg caggggctgt atagctggat gaaacagctc aacggcattt
    11341 acaacgtgcc gctggttacc atcatcatta tgggcttttt cttcccgcgc atcccggcgc
    11401 tggcggcaaa agtggcgatg gggattggca taatcagcta catcaccatc aactatctgg
    11461 tgaagttcga cttccatttc ctctatgtgc tggcctgtac gttctgcatc aacgtggtcg
    11521 tgatgctggt gatcggtttt atcaaaccgc gcgccacgcc gttcaccttc aaagatgcgt
    11581 ttgcggtgga catgaaaccg tggaaaaacg tcaagatcgc gtcaattggc atcctgttcg
    11641 cgatgattgg cgtctatgcc gggctggctg aattcggcgg ctacggtacg cgctggttag
    11701 cgatgatcag ttatttcatt gccgccgtag tgattgtcta cctgattttt gacagctggc
    11761 ggcatcgtca cgacccagcc gtaaccttta ctcccgacgc gaaggatagc ctatgaaacg
    11821 ccccaatttt ctgttcatca tgaccgatac ccaggccccc aatatggtcg gttgctatag
    11881 cggtaaaccg ctgaatacgc aaaatattga tagtctggcg gcggaaggta ttcgctttaa
    11941 ttccgcctac acctgttcac cggtttgtac accggcgcgc gccgggctgt ttaccggtat
    12001 ctacgctaac cagtccgggc cgtggaccaa caacgtcgcg ccgggtaaaa acatctccac
    12061 tatggggcgc tactttaagg atgccggcta tcacacctgt tacatcggca aatggcatct
    12121 cgacggtcat gactatttcg gcactggcga gtgtccgccg gagtgggacg ctgattactg
    12181 gttcgatggg gcgaactatc ttagcgaact gacggaaaaa gagattagcc tgtggcgcaa
    12241 tggcctaaac agcgtcgaag atttacaggc gaaccatatt gacgaaacct tcacctgggc
    12301 gcaccgcatc agcaatcgag cggtggattt tctgcaacag cccgcgcgcg ccgaggaacc
    12361 cttcctgatg gtggtttcgt atgatgagcc gcatcacccg ttcacctgtc cggtggagta
    12421 tttagagaaa tacgctgatt tttactacga gctgggcgag aaagcacagg atgacctggc
    12481 gaacaaacca gaacatcacc gcttatgggc gcaggcgatg ccatcgccag tcggtgatga
    12541 cgggctttat caccatccgc tctattttgc ctgtaatgac tttgttgatg accaaatcgg
    12601 acgggtcatc aatgccttaa agccagagca acgtgaaaat acgtgggtta tttatacctc
    12661 cgatcacggc gaaatgatgg gcgcacataa gctgatcagt aaaggggcgg cgatgtatga
    12721 cgacatcacc cgcattccgc tgatcatccg ttcgccgcaa ggggagcggc gacaggtcga
    12781 tacgccagtc agtcatatcg atttactgcc gacaatgatg gcgctggcag atattgaaaa
    12841 accagagatt ctgccggggg aaaatatcct tgcagtgaaa gagccacgcg gcgtgatggt
    12901 ggaatttaac cgctacgaga ttgagcatga cagctttggc ggttttattc cggtgcgttg
    12961 ctgggtgacg gatgacttta aactggtgct caacctcttc accagtgatg aactttacga
    13021 tcgccgtaat gatccaaatg aaatgcataa cctgatcgat gatgcccatt ttgccgacgt
    13081 tcgcagcaaa atgcatgatg ccttattgga ttatatggac aaaattcgcg atccgttccg
    13141 cagttaccaa tggagcctgc gtccgtggcg taaagatgca cagccgcgct ggatgggggc
    13201 atttcgtcca cgcccacaag atggttattc gccagtggta cgcgactatg acaccggcct
    13261 gccgacacaa ggggtgaagg tggaagagaa aaaacagaag ttctgacgat aatgcccgct
    13321 gatgaacacc cggcgggcat tattcaggct gcttaaacca gtgaaccgcc agtcaggaag
    13381 gaaaagataa ggaatatcag caaaatgctg cacacgatgc cgataccaaa aagcaaggta
    13441 tggaaagcca gcgtcccgcc gccaaaaacc agcgcgccgc gaatgcccca gcgattttcc
    13501 ttacgcatac cgccaataaa tgcgcaaagt agcgcagcaa tggccagtgc gataccgctt
    13561 tgatcgagga ttttatcgac atccacgtcc tcttttctct ccacggtggt aattttttcg
    13621 ccttttattc cggcgagaag acctctttta atttcagccg ttttgtccgc cacgatgctt
    13681 tcaagcgttg gcggtggtgc ggaaaaaggg ccgaatgaaa agtggataat ccccatcatt
    13741 aaagcgatgg caccgaatag catgcccaat gaactgattt tattttgcgc tatgataccc
    13801 atctgacctc cttgtagcgg atggcaaaag catatcagga aacgggcgga cgaaattctt
    13861 aaaaaaaacc gctaaaggta gagcgttagt gaaattgcaa aggagcaaca atgaaaattt
    13921 cccgcctcgg agaagcgcct gattaccgct tctcgctggc taatgagcgt acttttctgg
    13981 cgtggatccg tactgcacta ggatttctgg cggcgggggt tgggcttgat caacttgcgc
    14041 cagatttcgc cacgcccgtc attcgcgaac tgctggcgtt gttgttgtgt ctgttttccg
    14101 gcgggttagc gatgtatggc tacttgcgct ggttacgcaa tgaaaaagcg atgcgtctga
    14161 aagaggacct gccctacacc aacagcttat taatcatcag cttaatattg atggtcgtag
    14221 cagtgatcgt tatgggactg gtgttgtatg ccggatagcc gcaaagccag acgcattgcc
    14281 gacccagggc tgcagccgga gcgtacatca ctggcgtggt ttcgtaccat gctgggctac
    14341 ggcgcgttga tggcgttggc tatcaaacac aactggcacc aggcgggcat gttattctgg
    14401 atttcgattg gcatcctcgc catcgtggcg ctgatcctct ggcactacac tcgtaatcgc
    14461 aatttaatgg atgtcacgaa tagcgatttt tcccaatttc acgtagtccg tgacaaattt
    14521 ttgatctccc tcgcggtgtt atctctcgca atactgtttg ctgtaacgca tatacatcaa
    14581 cttatcgtat ttattgagag agtcgcatga caggaagtca ggtcattgat gctgaagagg
    14641 acaggcataa actggttgta gagtacaagg atgctctgca acctgccgat ttttatcaca
    14701 atttcaaaca acggggcatc cgctccgtac agcttattcc ccatcttgaa tttgatgacc
    14761 ggggcgatat gacggccgct tcggtgacag cagagctgtg ggggaaattt ttaattgccc
    14821 tgtttgagtg ttgggtgcgg gcagatatca gtcgtatctc gattgagctt tttgacgcca
    14881 cattacaaaa atggtgtggc agcgaaaacc cgcaaccgcg ccgtggctgc caggcgtgcg
    14941 actggcatct gctgtgtccg cacgctcggg aggagacgcc agacagcgtg ctttgtgcgg
    15001 gctatcaggc gttttattct tactctgcgc cgcatatgcg ggtgatgcgt gatctgataa
    15061 agcagcatcg ttctcccatg gagctgatga cgatgctgcg gtgacgtgag cttaaacggg
    15121 ctgcccctga tgcgacatcc gcgtcgccag cggtagccag cacagcacga tcaacaatcc
    15181 cattaaggtc atcaacaacc ccaggctgcc ctgaccggtt tgcggcaaca tcgcagagag
    15241 cgacgccagc acgccggaac caatgttttg tagaccgccg accagcgcgc cagccgtgcc
    15301 cgccaggaaa gggaacggct ccatcgcgcc gctggtcgcc agcggaaaca gcatcccggc
    15361 accgaaaaag aacagcgcgg cgggaacgag cagcgtccag acattcatca cgccaaacca
    15421 gtcggggatc cacatcagta agccagccag caggcagcag ataaccgact gccacatcaa
    15481 cgttgagaag cgtttattag gacgtccggc aaaccatgcc ccaaaaaatg ccgccggaat
    15541 cggcagaata aacaaaatac tgaccgtcat actgctcagc cctaacaccg cgcccatcag
    15601 cacgccggag caggcttcaa aggcggcaat cccggccaga ccgccaatca gcatcagcaa
    15661 ataacagtta aaaccgctgt taccgaaaag cgttttataa ctggtaagca ggcgcgtgcg
    15721 cggtgcgtcg accggacgcg tttccggcat ccagcgggcc atactgaagg tcacaccggc
    15781 acaaagtacc aacaaaaaga gataacaggc gcgccagttc cacatcgtat ccagcagacc
    15841 gccgattagc ggtgcgagca acggactgac aagaattccc atgtttaaca ggctgttagc
    15901 gtggcgcaac tgtgtccgtt catataaatc acgcggcaaa gtacgcgcca ttacgccgcc
    15961 aacgccggtg cccatcccct gcatcgcgct ggcggcaatc aatactgtca aactggaggt
    16021 cgtgatcgcg accagcgttg ccagcataaa aatggacatt ccgacgagga tcaccggtcg
    16081 gcgacccaca cggtcggaaa tcgggccata aaacagctgt gagacaccgt aagtcagcag
    16141 ataagcgccc attacgctct gcaccgcccc ttcacgaacg ttgagatcgc gcgccatatc
    16201 ggcaatagct ggaatataaa tggtttgcgc catctgaccg acggccacga gtaataccaa
    16261 catcaataac aaattgatgt ttctatgcct tttcattatc acggatgctt ttataaaaat
    16321 aattagaata agtcactacg cattcccata aatgcgggag aggaatctat cacattgata
    16381 aatgttagcc agatatacgc caggaatggg gaattgttta gcggcagaat atgtaaacaa
    16441 aagcggcaat aaatgttgcc gggagggatg gtaggtcgga taagatgcgt cagcatcgca
    16501 tccgacacca acccacacgc taaatataaa atgggagcgg ttacccgctc ccctttagtg
    16561 cgacttgaat ctgaattact tcaggtattt cagaacagca tcaagcagtt gcagtgctgc
    16621 aacaatgagt ttgaggataa gaatggcgat atccaccagg ctcatacgcg tctcctgtgg
    16681 ttcaggagca ctgccagctg acgtaccttt ccgctgcctg ttgccagctg ttgtgcacgc
    16741 ggtatctcaa ggagaaacgt tgtgctcgtt gacataacac agtgtgctca cgcggggtta
    16801 aggcgctgac ggcaccaccc gtttcagcca ggacttagtg cgccgggtaa cgatgcggcc
    16861 cccgcataac acattgcgta cagtgatatt atagaagctt gctgtataaa taaacagtaa
    16921 tatttggaca aaacgcaaac tgtgatcgat actcaactat gcactaaata cgtcaaaatt
    16981 cgtgccgaaa ttgcgcgttt tgcgcggaac acgtatactt tctgtgttga cataatacag
    17041 tgtgctttgc ggttaccagc cgcgggcggc tgacgaaacc tcgctccggc ggggtttttt
    17101 gttacctgca gtacagtaca aaacgtgatc aacccctcaa ttttcccttg ctgaaaaatt
    17161 ttccattgtc tcccctgtaa agctgtgctt gtataaatat tgttaaacac aaaaccaaca
    17221 aggtccccaa tgactacttc catgctcaac gcaaaactac taccaactgc gccatccgcc
    17281 gcagtggtcg tcgtgcgtgt ggtggtggtc gtcggcaatg cgccgtaggg actggaacaa
    17341 cacacgattc caaaaccccg ccggcgcaaa ccgggcgggg tttttcgttt aagcacctcc
    17401 cggaaagtcg gcccagaaga aaaggactgg agcatggcaa gttcgggcac atcatcgacg
    17461 cgtaagcgct ttaccggcgc agaatttatc gttcatttcc tggaacagca gggcattaag
    17521 attgtgacgg gcattccggg cggttctatc ctgcctgttt acgatgcctt aagccaaagc
    17581 acgcaaatcc gccatattct ggctcgccat gaacagggcg cggggtttat cgctcaggga
    17641 atggcgcgca ccgacggtaa accggcggtc tgtatggcct gtagcggacc gggggcgact
    17701 aacctggtga ccgccattgc tgatgcgcgg ctggactcca tcccgctgat ttgcatcact
    17761 ggtcaggttc ccgcctcaat gatcggcact gacgccttcc aggaagtcga cacctacggc
    17821 atctctatcc ccatcaccaa acacaactat ctggtcagac atatcgaaga actcccgcag
    17881 gtcatgagcg atgccttccg tattgcgcaa tcaggccgcc ctggcccggt gtggatagac
    17941 attcctaagg atgtgcaaac ggccgttttt gagattgaag cacagcccgc ggtggcagaa
    18001 aaagccgtcg cgcccgcctt tagcgaagaa agcattcgtg acgcagcggc aatgattaat
    18061 gctgccaaac gcccggtgct ttatctgggc ggtggtgtga tcaatgcgcc tgcgagagtg
    18121 cgtgaactgg cggagaaagc gcagctgcct accacaatga ctttaatggc gctgggcatg
    18181 ttgccaaaag cgcatccgtt gtcgctgggt atgttgggga tgcacggtgt gcgcagcacc
    18241 aactatattt tgcaggaggc ggatttgttg attgtgctcg gtgcgcgttt tgatgaccgg
    18301 gcgattggca aaaccgagca gttctgcccg aatgccaaaa ttattcatgt cgatatcgac
    18361 cgcgcagagc tgggtaaaat caagcagccg cacgtagcga ttcaggcgga tgttgatgac
    18421 gtgctggcgc agctgatccc gcaggtggaa gcgcaaccgc gtgcagagtg gcaccagttg
    18481 gtagcggatt tgcagcgcga attcccgtgt ccaatcccga aagcgtgcga tccgttaagc
    18541 cattacggcc tgatcaacgc cgttgccgcc tgtgtcgatg acaatgcgat tatcaccacc
    18601 gatgtgggtc agcatcagat gtggacggcg caggcttatc cgctcaatcg cccacgccag
    18661 tggctgacct ccggcgggct gggcacgatg ggttttggcc tgcctgcggc gattggcgcg
    18721 gcgctggcga acccggatcg caaagtgttg tgtttctccg gcgatggcag cctgatgatg
    18781 aatattcagg agatggcgac cgccagcgaa aaccagctgg atgttaaaat cattctgatg
    18841 aacaacgaag cgctggggct ggtgcatcag caacagagtc tgttctacaa gcagggcgtt
    18901 tttgccgcta cctatccggg aaaaatcaac tttatgcaga ttgccgccgg attcggcctc
    18961 gaaacctgtg atttgaataa cgaagccgat ccgcaggctg cattgcagga aatcatcaat
    19021 cgccctggtc cggcgctgat ccatgtgcgc attgatgccg aagaaaaagt gtacccgatg
    19081 gtgccgccag gtgcggcgaa tacagaaatg gtgggggaat aagccatgca aaacacaact
    19141 catgacaacg tgattctgga actcaccgtt cgcaaccatc cgggcgtaat gacccacgtt
    19201 tgtggccttt ttgcccgccg cgcttttaac gttgaaggca ttctttgtct gccgattcag
    19261 gacagcgaca aaagccatat ctggctactg gtcaatgacg accagcgtct ggagcagatg
    19321 ataagccaaa tcgataagct ggaagatgtg gtgaaagtgc agcgtaatca gtccgatccg
    19381 acgatgttta acaagattgc agtgtttttt cagtaaggag cgacattaaa ccgctcgggg
    19441 gagttactga tgagcgattt ttgatagatc cgttcagata tatagggtga aattgtgcca
    19501 gagaccatct caggcacaat ttattagccc aggacgttaa tttaagtcgc agcaccggta
    19561 acctgggggg gacagttagg gctaaaattt ccagcgatgg tgttaaattc tgattgctgt
    19621 ctataccagt ggtcatgacc agcattctat taattaatgg ttcatcgtga ggtatattat
    19681 ggcaagtcct ataaacagcg gaataatgat ggcaaatctc tgcccttcca cctttttgga
    19741 taaaaataga aatgtcgctg ctgagcttga tattaaaaat aacgagaaaa atattcacct
    19801 ggcagtaatt tcgcaaaatg gatgttgcag gaaataaagc gcttaatact aaacatcatg
    19861 tccggttctc ggagtattaa caccgatatt cttgactatt tccaccctat gccaggcacg
    19921 gaaaataatg gtaatcgtac ctggatggcg gcaactggag aggatgaata tatcgaaatc
    19981 aaacaaacag gtgataagtc ttttaatatt acactggttg gcagagataa accttcacga
    20041 aaagaaattc catattcagg agtggctgta gcgactatta ttaaatcgtt gtctgaaaaa
    20101 acttctgcgt tagaaacaca ttccgctgac acggtt
//
LOCUS       DAZUGK010000076        20133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZUGK010000076 DAZUGK010000000
VERSION     DAZUGK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.076"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..616
                     /locus_tag="ACHY5W_002363"
     CDS             <1..616
                     /locus_tag="ACHY5W_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447250.1"
                     /translation="LRSFTKARQGIIQFWAKNFAVVSKFCATALLPAHSTVRVFDANS
                     DDRRKYYGTDLRKALKRLGKDLAVPYINEQECERADPRGDGGFDIVATIEFEDKLSSN
                     FAFLGQCGAQERDWPKKTLEAHSLKLRTYFQVHFDIPTLMFTPVFYRNSDGQWVDNSP
                     CAGVLIIDRARILQLLKKTNHCPKIVSEQWFLDFEQIINDLKVE"
     gene            complement(617..1555)
                     /locus_tag="ACHY5W_002364"
     CDS             complement(617..1555)
                     /locus_tag="ACHY5W_002364"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4447251.1"
                     /translation="MPTVVSLFSGCGGSDAGVLRAGFNVLMANDILPYARDVYLENHP
                     ETDYILGDISGLQSFPSAELLIGCYPCQGFSQGGARKADRKINTLYLEFARALRKIKP
                     KAFIVENVSGMVRRNFEHLLKDQFKVFEEAGYTVSSQILNASHYGVSQDRKRIFIVGI
                     RKDYGITYKFPKPTHGDGLKPYSTIRDAIGHMPVWPIGEFYDADFHWYYLSRNRRQDW
                     DQISKTIVANPRHMPLHPISPTLEKLGPDKWQFTSDAPARRFSYREAAILQGFGDLIF
                     PETERASINMKYTVVGNAVPPPLFEAVAKSLPDIWD"
     gene            complement(1807..2040)
                     /locus_tag="ACHY5W_002365"
     CDS             complement(1807..2040)
                     /locus_tag="ACHY5W_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447252.1"
                     /translation="MHRIPGEIPQHKTKNIKLMAIVHRLQQIMVNENLTPDELVGCAE
                     MVRDNYRRFNDISRQKIEVFRPANMPKKLPHRM"
     gene            complement(2040..2492)
                     /locus_tag="ACHY5W_002366"
     CDS             complement(2040..2492)
                     /locus_tag="ACHY5W_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFS4447253.1"
                     /translation="MAKIHEVKLHAKYFDLVLEGKKRAEFRKNDRNYERGDTLILHEW
                     VQGVFTGRKVEARITDVTDLSDWLEDYVLLSIELLNTSAYEIVNWKELSERGLVFRIN
                     HEIMHQLGLAVMYEPETGVSGGAMVATDGAWNYSDEQMERAQQNGWLG"
     gene            complement(2471..4777)
                     /locus_tag="ACHY5W_002367"
     CDS             complement(2471..4777)
                     /locus_tag="ACHY5W_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFS4447254.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWAHQLKGQRMRWHEALLIAVGAVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNHEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPHQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSERAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRTPNRQ
                     QRNGSPLKPNEIAPSARLTRSERMQITRIRVDLAQNGIWPQRWELEALARGATVNYDG
                     KKFTYLVADVWPGFSTVMEWTSWQKFTR"
     gene            complement(4767..5042)
                     /locus_tag="ACHY5W_002368"
     CDS             complement(4767..5042)
                     /locus_tag="ACHY5W_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFS4447255.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNVGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(5039..5263)
                     /locus_tag="ACHY5W_002369"
     CDS             complement(5039..5263)
                     /locus_tag="ACHY5W_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFS4447256.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(5266..5565)
                     /locus_tag="ACHY5W_002370"
     CDS             complement(5266..5565)
                     /locus_tag="ACHY5W_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFS4447257.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(5565..5789)
                     /locus_tag="ACHY5W_002371"
     CDS             complement(5565..5789)
                     /locus_tag="ACHY5W_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFS4447258.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(5853..6353)
                     /locus_tag="ACHY5W_002372"
     CDS             complement(5853..6353)
                     /locus_tag="ACHY5W_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFS4447259.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(6350..6547)
                     /locus_tag="ACHY5W_002373"
     CDS             complement(6350..6547)
                     /locus_tag="ACHY5W_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447260.1"
                     /translation="MAFVDGVVIMSEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIR
                     TKKQSPLKKALTFLKSRRVWK"
     gene            complement(6523..6795)
                     /locus_tag="ACHY5W_002374"
     CDS             complement(6523..6795)
                     /locus_tag="ACHY5W_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFS4447261.1"
                     /translation="MEVNDYVIQYPIDAVHTVKFAELLGKPETAVVKMVKENKLPVIE
                     LRDPSKPNARVGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            6948..7241
                     /locus_tag="ACHY5W_002375"
     CDS             6948..7241
                     /locus_tag="ACHY5W_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4447262.1"
                     /translation="MQTFEKLKAIRKAEGLTQAKFSEISGIALGTVKNYESGHKDPGL
                     SIVMRVTNTPLFKKYTLWLMTGDTSPQAGQIAPALAHIGQKPTESDHSEKQTG"
     gene            7311..8291
                     /locus_tag="ACHY5W_002376"
     CDS             7311..8291
                     /locus_tag="ACHY5W_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4447263.1"
                     /translation="MSIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEAVAFERYTIA
                     YASQKEWAGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLDGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(8477..8977)
                     /gene="cpxP"
                     /locus_tag="ACHY5W_002377"
     CDS             complement(8477..8977)
                     /gene="cpxP"
                     /locus_tag="ACHY5W_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFS4447264.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            9127..9825
                     /gene="cpxR"
                     /locus_tag="ACHY5W_002378"
     CDS             9127..9825
                     /gene="cpxR"
                     /locus_tag="ACHY5W_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFS4447265.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            9822..11195
                     /gene="cpxA"
                     /locus_tag="ACHY5W_002379"
     CDS             9822..11195
                     /gene="cpxA"
                     /locus_tag="ACHY5W_002379"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFS4447266.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSFSV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(11311..11985)
                     /gene="yiiM"
                     /locus_tag="ACHY5W_002380"
     CDS             complement(11311..11985)
                     /gene="yiiM"
                     /locus_tag="ACHY5W_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFS4447267.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(12134..13117)
                     /gene="kdgT"
                     /locus_tag="ACHY5W_002381"
     CDS             complement(12134..13117)
                     /gene="kdgT"
                     /locus_tag="ACHY5W_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFS4447268.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASHIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(13377..13997)
                     /gene="sodA"
                     /locus_tag="ACHY5W_002382"
     CDS             complement(13377..13997)
                     /gene="sodA"
                     /locus_tag="ACHY5W_002382"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFS4447269.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            14282..15316
                     /gene="rhaT"
                     /locus_tag="ACHY5W_002383"
     CDS             14282..15316
                     /gene="rhaT"
                     /locus_tag="ACHY5W_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFS4447270.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(15313..16161)
                     /gene="rhaR"
                     /locus_tag="ACHY5W_002384"
     CDS             complement(15313..16161)
                     /gene="rhaR"
                     /locus_tag="ACHY5W_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFS4447271.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSVSAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(16235..17071)
                     /gene="rhaS"
                     /locus_tag="ACHY5W_002385"
     CDS             complement(16235..17071)
                     /gene="rhaS"
                     /locus_tag="ACHY5W_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFS4447272.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDTVADQFSLSLRTLHRQLKQQTGMTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            17359..18828
                     /gene="rhaB"
                     /locus_tag="ACHY5W_002386"
     CDS             17359..18828
                     /gene="rhaB"
                     /locus_tag="ACHY5W_002386"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4447273.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            18825..20084
                     /gene="rhaA"
                     /locus_tag="ACHY5W_002387"
     CDS             18825..20084
                     /gene="rhaA"
                     /locus_tag="ACHY5W_002387"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4447274.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
BASE COUNT         5071 a         5103 c         4915 g         5044 t
ORIGIN      
        1 gttacgttct ttcacaaagg ctaggcaagg aataatacag ttttgggcaa aaaattttgc
       61 tgtcgtaagt aaattttgtg caacagcact actcccagca cactcaactg tacgagtatt
      121 tgacgcaaac tcagatgaca ggcgaaaata ttatggtact gatttaagaa aagccttgaa
      181 aaggctagga aaagacttag ctgttcctta tattaatgag caagagtgcg aaagagctga
      241 tccgagaggt gatggtggtt ttgatatagt tgcaactata gaatttgaag ataaattaag
      301 tagtaacttt gcctttttag gtcaatgtgg agcccaagaa agagattggc ccaaaaaaac
      361 acttgaagcc cactcattga aactacgaac atattttcaa gtgcattttg acataccaac
      421 tctaatgttt acccccgtat tctatcgaaa ctcagatggt caatgggtag acaatagccc
      481 ttgcgctgga gtattgatca ttgacagagc aaggatatta cagcttttaa aaaagacaaa
      541 ccattgccct aaaatagttt cagaacaatg gtttcttgat ttcgaacaaa ttatcaatga
      601 tcttaaggtc gaataactaa tcccaaatat ctggaagaga tttagcaacc gcctcaaata
      661 aaggaggcgg aacagcattg ccaactactg tatacttcat attaatagaa gcccgttcag
      721 tttctggaaa aattaggtct ccaaaaccct gtaaaatagc ggcctctcga taactaaacc
      781 tacgagctgg tgcatccgaa gtaaattgcc acttatctgg acccaatttt tctaatgttg
      841 gacttattgg atgtagaggc atatgtctag gatttgcaac aattgtttta gatatctgat
      901 cccaatcttg ccgacggttg cgcgatagat aataccaatg aaaatcggca tcataaaact
      961 caccaatagg ccaaacaggc atatgaccaa tagcatcacg aattgttgaa tatggtttca
     1021 agccatcacc atgtgttggt tttggaaatt tgtatgtaat accatagtct tttcgtattc
     1081 ctacgataaa gattctcttc ctatcttgtg atacgccata gtgggacgca ttcagaattt
     1141 gcgagcttac tgtataacct gcttcttcga aaactttgaa ttgatccttt aacaaatgtt
     1201 caaagttacg ccttaccatt ccagaaacat tctctacaat gaatgctttt ggcttaatct
     1261 ttctcaaagc acgagcaaac tctaaatata gagtattaat ctttctatct gccttccttg
     1321 ctccaccctg gctaaatccc tggcaaggat agcatccgat aagcaattcg gcagaaggga
     1381 acgattggag tcctgaaata tcgcccaata tgtaatcagt ttcaggatgg ttttctaagt
     1441 aaacgtccct tgcataaggc aaaatatcgt ttgccatcag cacattgaac cctgctctca
     1501 aaactcccgc atcagaacca ccacatccag aaaaaagtga aactacagtt ggcatcgccc
     1561 ctcctaaaaa ccgaccgcgt attatagcga aaccacccct tgggaaaagc tagatttcgc
     1621 caagttttga tattctcatg ttttagtagt tgtggccatc tttaacgaga gaaagatgaa
     1681 attgagttct cattaatttt caataggttt aattgtaaac acagcaggaa tcaaagctaa
     1741 cacgtcgcta cgctcattgt tcaaccccgc catctctgaa aacaagtttc agaaatggcg
     1801 agtatactac atcctatgcg gtagtttctt aggcatgttt gctggtcgaa atacctcaat
     1861 tttctgtcta ctgatatcgt taaaccgtct gtaattatcc cggaccattt cggcacaccc
     1921 gaccagctca tcaggcgtca gattttcgtt gaccataatc tgctgtagac ggtggacaat
     1981 ggccatcagc ttgatatttt tagttttatg ctgcggtatc tcgcctggta ttctgtgcat
     2041 tatccaagcc acccgttttg ctgtgcacgc tccatctgtt catctgaata gttccatgct
     2101 ccatccgtgg caaccattgc cccgccagac acccccgtct ctggttcata cataacagca
     2161 aggccgagct gatgcataat ttcatgatta attctgaata ccaggccacg ctcactaagt
     2221 tctttccagt tcacaatctc atatgcgctt gtattaagca gctcaatact tagcaagaca
     2281 taatcttcca gccagtctga caggtcagta acatctgtta tccgggcttc aacctttcgc
     2341 cccgtaaaca caccctgtac ccattcatgc aaaatcagcg tatccccgcg ctcataatta
     2401 cggtcatttt tccgaaactc tgcacgtttc tttccttcca gcacaaggtc gaaatatttt
     2461 gcgtgcagct ttacctcgtg aatttttgcc atgatgtcca ctccattact gttgagaatc
     2521 ccggccacac atcagcgacc agatacgtga atttttttcc gtcataattt acggttgctc
     2581 cacgcgccag cgcctcaagt tcccatcgct gaggccagat gccgttctga gcaaggtcaa
     2641 cacggatacg ggtaatttgc atacgctccg accgggtcag cctggccgat ggcgctattt
     2701 cattcggttt taacgggctt ccgtttcttt gctgacggtt tggtgttctc aggccgtgtt
     2761 ttaatgcgcc cctgagcgcc ctcacgacct ccgggtcatt ccattcgata acaccgtcat
     2821 caaccagatt aagcacggct gcggcgcgct cagaaggtgt gggagccggt aacgaagtat
     2881 caccaccggt gagctttcca cagttattga caggactccg aggcgcggcg atgccgcttt
     2941 ttaaagtcaa aggctcaacg accggcactt tcggaacaat gcgccagtcc gtcgttctgg
     3001 tgatatgaat atgacgcgcg ccgagatgcg gcgcgtaaat gccgaccact ctctcgactt
     3061 cttcctcgta ctcgttaact tcatccgacg gactacgggc gaccctgaca gtctgacaat
     3121 cgcgcgggac atttgcccca ccctgcgcgc tgatatacag cgcaaaatca ccactgtctg
     3181 cggcggcgcg tgcagcctcg acgcgttcgt caaactcatc agcaatgctg acgccgcgag
     3241 gcagtttgcg tagttcacgg taagccccca ttgtcggcag gccaaccgtt ttaaattgtg
     3301 ggatacgcca cgttgacgcc catgcggtaa cagccgcagc agtgtctttc agcggtctgc
     3361 cggtatcgtt atcgagctga ccatccagtg catagccatc gatgtttttt gagatgtatt
     3421 tcgcgatata tcccgcagca ccgccccggt taaggtgttt tgcctgaaaa cggtttcgcg
     3481 cggctcctct ttcgtcgcca tcctctttga gcgcataacg acgcatgatt tcaataatct
     3541 ggttacgctg gtgtggatta caaaaaagca tcatatgcca gtgcggcgtt ccgtcgtggt
     3601 gtggctcgac gacacgcaaa ccgtagacct gtaaatcatt atccttgaat gccgtgcgca
     3661 tcaggctcca gatacggcag agataacgct gcgcatcctt tggattaaat gcctcatggt
     3721 tccagccgtg attaagctgg acggttttac tttcgccttt tccgacctga cgtgtcgggt
     3781 gatactttga cggcgcggtc agcgtgataa acatccccac atcaccctct gcggcggcgt
     3841 aacgctcaat accggcaatg gtgttcatca gctccatccg gcgaatttca ggattagaaa
     3901 tactgcccat caccttactg ataaggtcga tgcgttcgcc ggtttccctg ttttcaaggt
     3961 cacacgattt aagaaattcc agatttgcct ggcggcgtgc acgtacatca cgaatggcat
     4021 gtttactggc ataaggagaa cggtctttat tcaccgcccc gacagcaatc agtaacgcct
     4081 catgccagcg catacgctgg cctttaagct gatgagccca ccactcatcg ttaaacagac
     4141 gggcaatggc agaatatgcc tgcctcgtgg tcatctgccc tttacggtat tttttccagt
     4201 agagagggga aatattgaaa gctcgtgcag cgccagcaac atgaccatac aggtgagcct
     4261 gcgcctcatc cgtaaacagc gattctttct cgccatgcgc atccacccag gcatcgctga
     4321 gttcctcata catcatgaaa agctgcgatg agatacgggc ggcaaatttt ttcagctcct
     4381 tgtcattcat tcccggcagg cgcgcatagt ggtcacgctc tgccagaaac agtaacgacg
     4441 cgtcggtgtt catttcatgg cgctgattca cacgctcaat gcgcggccat aaacgacgct
     4501 gaaaagtgga tgtgagaaaa taaaacccgt gcaccgggct tttattgcgc cggatgtagt
     4561 catagcgtga agtaaacagc gagcgcaaaa agtaaggcag gcggttaatc gtggataaaa
     4621 caccttgcac ctgacgcatc tcgtcacgtg taaggggtct ttcgcgcccg acagcctcgc
     4681 gtggcgcgtt ccatgcataa gcaccggtaa acgccttacc ggtgcctgcg gcaaatgctg
     4741 acggagggac aaaacgcccg gaggctttaa cggccatatg agccaaaagc ctctgaacaa
     4801 cgcttgctga gttgctcaac ctgcgcgttt aaatcagcaa aagactttgc gcttccggtc
     4861 agaatatcgt gatgcatcag gccggaaacg agctggctta atttcgggta ataaccaacc
     4921 actgccagcc attcctgacc gacgttttta ccgctttccg ctctcttttt ctcgtggaga
     4981 ataaactgaa agctgtcact ggtaacgaca taacgttcgc caatttcaat acgaatactc
     5041 atgccgttct ccggtaatgt ttgttttttg cttcaaagac tgactgacag gaaacacaac
     5101 gcgtggctga cggataagcc gcacgacggg cagcaggtat tggcgcgtca cactcttcgc
     5161 aaaccagcgc agaagcaccg caatgtttta cccttgccgc gttaatctgg cgctccagta
     5221 attcagcctg ttgttcctga ataaaatcta cgttgtccgg cattatcagc tccttttatc
     5281 gttaagtttc ctggatacat cagcgcaata actggcaagt tctgtcgtta attttgtcag
     5341 ttcatccacg gaggaaattt gcttgtggaa tacagcgcgt ttaacaagta aattgaccac
     5401 atcagacagg aggtttaatt cattctgata aatcgcgata acagattcag ttatgtcgcg
     5461 tttttcttta tcaagacaaa gttgaataag agacaaatcg ccatttttca taacggcgat
     5521 ttttaaggcg ttattcagta atacaactga atgagaacag gacatcaaag cacctccccg
     5581 cgagacaatc cgatattgtg aaatttttcc gactcctgac tgagcagctc gactatctcc
     5641 acgcgggata actccgcctt tgtgatgtgg cgaatcatgg catcaagatg agaagaaaag
     5701 cgcgtcgccg cgtcggcctg tgcttcggtt ctggcctgtt gcagcagtaa tgcgtattta
     5761 ccgcactgat tttcagaaac tgtatgcatg actttctcca ggcaaaaaga tgccccgcac
     5821 gattaagtgc gttaaaaact ctggttaatt acttaatgca gatattgctc tggttttacc
     5881 gacgtcagaa ttgtcggtgc atattcaaac agactgaata attcacgtaa tgcacggaat
     5941 aaagcatcac gccagtaaca tgactcttca ttaattcgcc agtatggctg attgaattct
     6001 ttttctgtca atcccgcatg cataaataaa gtgcgacgct gactgactgt taaaaaacta
     6061 atatatgcat actcactcgc gccaacctga cgacgttttg agaatgcccc acgcaattca
     6121 tcaattgcac ataccagtcg ttcacgttcg acgtcgttca tttcttcaaa acgcatcgtt
     6181 gcgtgacgct gttttaactg cgcatgaaag caaaccgtta gccgttcgcg ctccatcatc
     6241 tgattataat aatcacatgt atcctgccag cgaggaacgg caagatgctt accaattatc
     6301 cggcgcatag ctgctggctg tttttcaacg agattgagcg tcatcactgt catttccata
     6361 ccctccggct tttcagaaag gtcagagcct tttttaacgg actctgtttt ttggtgcgga
     6421 taatgattcc cttacgcccc ttaccgtggg tgatggtgaa gtcaatcgcc ctggggcttt
     6481 cgttacgcag taactgagca atacaacgcg gttcactcat aatcacaacc ccatccacaa
     6541 aagccatgca tcacgctgtt caactggtcg gttataaaac gcctctcgta cagcgcgatt
     6601 aaactctgga atgaaaaccc acttctcacc gacacgagcg ttcggcttac ttggatcacg
     6661 aagctcaata actggcaatt tattctcttt taccatcttg actacagccg tttctggctt
     6721 accaagtaac tctgcaaact taaccgtatg taccgcatca atcgggtact gaatcacata
     6781 gtcattgact tccattgatt agcccttttt gctttcgtgt tacccttatt agatccagtc
     6841 ccttctaggt cgcccctgtc ctttctaggg actggctaac acacccaaaa ggtcaccaat
     6901 acacaacctt ttgacgggaa tataagtcac caataggtta ctgtcaaatg cagacattcg
     6961 aaaaactgaa agcgattagg aaagcagaag gcttaacaca ggcgaaattc agcgaaatta
     7021 gcgggatagc tctaggaaca gtcaaaaatt acgaaagtgg gcataaagac cctggtctca
     7081 gcatcgttat gcgagtcaca aatacgcctt tatttaaaaa atatacgctc tggttaatga
     7141 ctggtgatac gtcaccacaa gctggtcaga tcgcgccggc tctcgcacac attgggcaaa
     7201 aaccaacaga atcagaccac tccgaaaaac agactggtta aaactctata aacattacat
     7261 tttcactatt tgttaccaag atggtgaata cagcgtcaga gggctttctt atgtcaatta
     7321 agaagctcga tgatggacgc tatgaagtgg acattagacc tcgcggtcgc gacggaaaac
     7381 gcatccgcag gaaatttgaa agaaaagctg aggctgtagc atttgagcga tacacaatcg
     7441 cctacgccag ccagaaagaa tgggcaggtc agcgagcaga tcgcagaact ttgagtgagt
     7501 tgctggacat ctggtggaaa tatcacgggc aaaaccacga gcatggaaca aaagagttta
     7561 atcatctgct caaaaccatc agcggcatag gtgatatacc agtgagccgg atgagcaaaa
     7621 gagctttgat ggattatcgt tccatgcgac tacgtgatgg tatcagtgcc gcaacgataa
     7681 accgtgacat gtaccgatta tccggcatgt tcacaaaatt aattcaattg gatgaatttt
     7741 ccgggcaaca cccaattcac ggactgccgc cactggcgga ggccaaccct gaaatgacgt
     7801 tcctggaaaa agcagaaatc gaaaaactgt taaatgtttt ggatggtgat gacttacttg
     7861 tcgcactttt atgtctgagc actggaggaa gatggacgga agttgccacg ctaaaaccag
     7921 cacagattac aaattgcagg gttaccttcc tgaaaaccaa aaacggtaaa aagcgaaccg
     7981 tgccgatttc tgaggaactg gagaaaaaag ttaaagagga ggccagcgct aaattattca
     8041 aagttgatta tgagaagttt tgcgggattt tacgcagagt gaagccagat ataccaccca
     8101 atcaggcaac ccacatcctg cggcatacat tcgcaagcca tttcatgatg aatgggggca
     8161 atataatcgc actgcaacag attctgggac atgcgagcat tcagcagacg atggcctatg
     8221 cgcaccttgc gcctgactac ctgcaaaatg ccgtcgcact gaatcctcta aaaggcggag
     8281 tgacgttata aatttccctt ctgagtgtcc acatagtgtc cacactctca gaactttgta
     8341 gcccttccag tcccttatag gttttcttaa gttactgttt tcttacggaa accgatgtaa
     8401 gtgattgata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca
     8461 aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg
     8521 aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcgt
     8581 ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca
     8641 tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt
     8701 tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa
     8761 caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt
     8821 cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac
     8881 ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca
     8941 gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg
     9001 tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa
     9061 aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt
     9121 taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag
     9181 gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt
     9241 gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat
     9301 ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg
     9361 gcgcgcggca gcgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc
     9421 ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg
     9481 cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc
     9541 ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc
     9601 ggtactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt
     9661 gaacatttaa gccaggaagt gctgggcaaa cgcctgacgc ctttcgaccg cgctatcgat
     9721 atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt
     9781 aaaaccttgc gtggtcgcgg ctacctgatg gtttctgctt catgataggc agcttaaccg
     9841 cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt
     9901 tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgagcag cgtcaggggc
     9961 tgatgattga gcagcatgtt gaagcggagc tggcgaacga tccgcccaac gatttaatgt
    10021 ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat
    10081 tagtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta
    10141 actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg
    10201 aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc
    10261 gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac
    10321 tgctgattgt caccatgctg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg
    10381 caaaaccggc gcgtaagctg aaaaatgctg ccgatgaagt tgcccaggga aacttacgcc
    10441 agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc
    10501 agatggtcac cgcgctggag cgcatgatga cttctcagca gcgtctgctt tctgatatct
    10561 ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc
    10621 gtagcggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca
    10681 tgatcaacga tctgttggtg atgtcacgta atcagcagaa aaacgcgctg gtaagcgaga
    10741 cattgaaagc taaccagttg tggggcgaag tgctggataa cgcggcgttc gaagcagaac
    10801 agatgggcaa gtcgttttct gttaacttcc cgcctgggcc gtggccgctg tacggcaacc
    10861 cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata
    10921 cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg
    10981 atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg
    11041 atgaagctcg cgatcgtgaa tctggcggta caggtttggg actggcgatt gttgaaaccg
    11101 ccattcagca gcaccgtggt tgggtgaagg cggaagacag cccgcttggc ggtttacggc
    11161 tggtgatttg gctaccgctg tataagcgga gttaaacact gcgcagggca gcttttgtcg
    11221 gccgaataag gcgtttacgc cgcatccggc atttgagcag aatgcctgat gcgacgctgg
    11281 tcgcgtctta tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc
    11341 ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc
    11401 ggcggcggag agtaaacggt gatattgatc gtcatcaaac ggcatatgcc atgcgatggc
    11461 ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc
    11521 agacactttc cctggcgcta tcacgctata caaccatccc actttacccg tgttttgcat
    11581 caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg
    11641 ctggctgacc tgaattaatg cctctcccca gcggaaaata tcgccaatat aaacattact
    11701 ttccgtgaga ccgtcggttg agagattttc gccaaacgca ggcgcaacaa acaactccgc
    11761 ctgttccgga aattcccgcg cccagtagag atagtgctca cgaggataat gacacagcgc
    11821 tctgtctggc ccaccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc
    11881 cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg
    11941 ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccctctccac
    12001 gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag
    12061 tatccaggca ggaactcaat agcaaaccgg acgaacgtta gttcatccgg cggtggggat
    12121 taaggaagca ggtttatttc accgtaccta aaatttcgat tttcgccgct ctggctttga
    12181 ctttacgtga ccagatagag gtaagaattg gcaccagaat cgaagtcaca atgactgccg
    12241 tcgctaccag cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa
    12301 tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc
    12361 catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta
    12421 ctgccacacc caacaggatc cccagcaaac ctgtctgggc aattacagtc aaatcaatag
    12481 tattgcctag tgcgaaggca aagaatggaa tcagcgtctg caccgctttg ctgaaaaatt
    12541 ctcgtaattc agggtcaagg ttcccaagag caaagcccac caggaacggt aatactgcgc
    12601 cgacgaaaac atgcggttca aacgaggcaa tcccggcagt gcccagaata atcatcgtca
    12661 tgagcggccc ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt
    12721 actgctgcat gatggaagcg taaagtccgc cgttggtcat atccatcgcc gccaccagcg
    12781 ccagcgttga aagtccggca aagaatccaa cttcaacacc atgttccgga ataatgtgcg
    12841 aggcaattgc cgcaaccacc cacgcgacgg caattttagt taccaccagc gtaccggatt
    12901 tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca
    12961 ccgccagaat gggcaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc
    13021 ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc
    13081 cccccgggat tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt
    13141 ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt tgatggaaac gaagatagac
    13201 attcatttaa tcaataaaag tgatcaaaac cacaccaatt gaaacgctgt tttattttta
    13261 tcggatcatt gtttttattt tgaaattgat cacaaaaaaa ccaccgccat tggcaatggt
    13321 tcatttttta agctgatatg cggcgtaaac gcctcattgc agcagacggc aaatgattat
    13381 tttttcgccg cgaaacgtgc cgctgcttcg tcccagttca ccacgttcca gaactctttg
    13441 atgtagtccg ggcggcggtt ctggaatttc aggtagtaag catgttccca cacatccagg
    13501 cccagaatcg ggaagccgga tgcgccagaa atagcttcac ccatcagcgg ggagtcctgg
    13561 ttagcagtag aaaccaccgc cagtttatcg cctttcagca ccagccatgc ccagccggaa
    13621 ccaaagcggg aagctgccgc tttctcaaat tctgctttga agttatctac ggaaccgaaa
    13681 tcacgttcga tagccgcttt caggtcaccc tgcagggtgg tgcctttttt cagacctttc
    13741 cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct
    13801 gctggcagct ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc
    13861 aggctttcca gcgccgcgtt ggcgttgttg acgtaggtct gatggtgttt ggtgtggtgg
    13921 atttccatgg tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat
    13981 ggcagggtat agctcatatt catctccagt attgtcgggc ggccgattgt taatgccgcg
    14041 taagcagttg gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac
    14101 ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt
    14161 aatgccgtga ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc
    14221 acgattttta cactcatctc gtcggagatg tgacgcgacg aaaaatgatg aggataagaa
    14281 gatgagtaac gcgattacga tggggatatt ttggcatttg atcggcgcgg ccagtgcagc
    14341 ctgtttttac gctccgttca aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt
    14401 cggtgggatt gtttcgtgga ttattctgcc gtgggccatc agcgccctgt tactaccgaa
    14461 tttctgggcg tattacagct cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg
    14521 cgctatgtgg gggatcggta atatcaacta cggcctgacc atgcgttatc tcggcatgtc
    14581 gatgggaatt ggcatcgcca ttggcattac gttgattgtc ggtacgctga tgacgccaat
    14641 tatcaacggc aatttcgatg tgctgattaa caccgaaggc ggacgcatga cgttgctcgg
    14701 cgttctggtg gcgctgattg gcgtagggat tgtaactcga gccgggcagt tgaaagagcg
    14761 caagatgggc attaaagccg aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat
    14821 gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga
    14881 agccgctgcc gcacttggcg tcgatccact gtatgtcgct ctgccaagct atgttgtcat
    14941 catgggcggc ggcgcgatca tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa
    15001 ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt
    15061 actctcggca ctgggcgggt tgatgtggta tctgcaattc tttttctatg cctggggcca
    15121 cgcccgcatt ccggcgcagt atgactacat cagttggatg ctgcatatga gtttctatgt
    15181 attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg aacaatgcag gacgccgtcc
    15241 ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc gccgctaaca tcgtcggcat
    15301 cggcatggcg aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc
    15361 ggtttcccgg gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga
    15421 aatatcactg attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac
    15481 ctgccgcaga tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac
    15541 gcgctcactg cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct
    15601 ggccgccagc cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaatga
    15661 atcactggtg taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactctgc
    15721 catttcgtta gcaaacggca catgctgact actttcatgc tcaagctgac cgataatctg
    15781 ccgcgcctgt gccatcccca tgctacctaa gcgccagtgt ggttgccctg cgctgacgct
    15841 aaatcccgga atcgccccct gccagtcaag attcagcttc agccgctccg ggcaataaat
    15901 aatattctgc aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat
    15961 gtaaaagaga tcgccacggg taatgcgata agggcgatcg ttgagtacat gcagaccatt
    16021 accgcgccag acaattacca gctcacaaaa atcatgtgta tgttcagcaa agacatcttg
    16081 cggataacgg tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag
    16141 aagttttaat tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc
    16201 aatatggcgt acaaatacgt tgagaagatt cgcgttattg cagaaaacca tcccgtccct
    16261 gacgaatatc acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac
    16321 tgtcgctgaa tccacagcga taggcgatgt cagtaacgct ggcctcgctg tggcgtagca
    16381 gatgtcgggc tttcatcagt cgcaggcggt tcaggtaacg ctgaggcgtc attcccgttt
    16441 gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga aaattgatcc gccacggtat
    16501 cccaattcac ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg
    16561 atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca
    16621 taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca
    16681 tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgagacg
    16741 gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc
    16801 gatccggcga gcgatatagc acattggtca gacacagatt atcggtatgt tcatacagat
    16861 gccgatcatg gtcgcgcaca aaacagaccg tcccaccggt gatggtatag ggctgcccat
    16921 taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat
    16981 gttcagggaa atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg
    17041 gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg
    17101 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa
    17161 acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc
    17221 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
    17281 tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa
    17341 attcagcagg atcacattat gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc
    17401 agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa
    17461 atccatcgtt ttaacaatgg gctgcatagt cagaacggct atgtcacctg ggatgtggat
    17521 agccttgaaa gtgccattcg ccttggatta aacaaggtgt gcgaggaagg gattcgtatc
    17581 gatagcattg ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag
    17641 cgtgtgggcc tgcccgttgc ttatcgcgat agccgcacca atggcctgat ggcgcaggca
    17701 caacagcaac tcggcaaacg cgatatttat cagcgtagcg gcatccagtt tctacccttc
    17761 aatacgcttt atcagttgcg tgcactgacg gagcaacaac ctgaactcat tccacacatt
    17821 gctcacgctc tgctgatgcc ggattacttc agttatcgcc tgaccggcaa gatgaactgg
    17881 gaatatacca acgccacgac cacgcaactg gtcaatatca atagcgacga ctgggacgag
    17941 tcgctactgg cgtggagcgg tgccaacaaa gcctggtttg gtcgcccgac gcatccgggt
    18001 aatgtcatag gtcactggat ttgcccgcag ggtaatgaga ttccagtggt cgccgttgcc
    18061 agccatgata ccgccagcgc ggttatcgcc tcgccgttaa acggttcccg cgccgcttat
    18121 ctctcttctg gcacctggtc attgatgggt tttgaaagcc agacgccatt taccaatgac
    18181 acagcactgg cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg
    18241 aaaaatatta tgggcttatg gctgcttcag cgagtgcttc aggagcggca aatcaacgat
    18301 cttccggcgc ttatcgccgc gacacaggca cttccggctt gccgcttcat tatcaatccc
    18361 aatgacgatc gctttattaa tcctgagacg atgtgcagcg aaattcaggc tgcgtgtcgg
    18421 gaaacggcgc aaccgatccc ggaaagtgat gctgaactgg cgcgctgcat tttcgacagt
    18481 ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc agctgcgtgg tgaagatttc
    18541 tcgcaactgc atattgtcgg cggcggctgc cagaacacgc tgctcaacca gctatgcgcc
    18601 gatgcctgcg gtattcgggt gatcgccggg cctgttgaag cctcgacgct cggcaatatc
    18661 ggcatccagt taatgacgct ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc
    18721 agcactaccg cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcccactat
    18781 gtggcgcaga ttcactctac acgacagaca aaggagcttt gcgcatgacc actcaactgg
    18841 aacaggcctg ggagctggcg aaacagcgtt tcgcggcagt agggattgat gtcgaggagg
    18901 cgctgcgcca acttgatcgt ttacccgttt caatgcactg ctggcagggc gatgatgttt
    18961 ccggttttga aaacccggaa ggttcgctga ccggagggat tcaggctact ggcaattatc
    19021 cgggcaaagc gcgtaatgcc agtgagctac gtgccgatct ggaacaggct atgcggctga
    19081 ttccggggcc aaaacggctt aatttacatg ccatctatct ggaatcagac acgccagtct
    19141 cgcgcgacca gatcaaacct gagcacttca aaaactgggt tgaatgggcg aaagccaatc
    19201 agctcggtct ggattttaac ccctcctgct tttcgcatcc gctaagcgcc gatggcttta
    19261 cgctctcgca tcccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc
    19321 gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga
    19381 tcccggatgg tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg
    19441 cagcactgga tgaggtgatc agcgagaagc taaaccctgc gcaccatatc gacgccgttg
    19501 agagcaaatt gtttggcatt ggcgcggaga gctacacggt tggctccaat gagttttacc
    19561 tggggtatgc caccagccgc cagacggcgc tgtgcctgga cgccgggcat ttccacccga
    19621 ctgaagtgat ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc
    19681 acgtcagccg tccggttcgc tgggacagcg atcacgtggt gctgctggat gatgaaaccc
    19741 aggcgattgc cagtgagatt gttcgtcacg atctgtttga ccgggtgcat atcggcctcg
    19801 acttctttga tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga
    19861 aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg
    19921 cgggcgatta cactgcgcgt ctggcgctac tggaagaaca gaaatcgttg ccgtggcagg
    19981 cggtatggga aatgtattgt cagcgtcacg atacgccagc gggtagcgaa tggctggaga
    20041 gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg gtaaacactg ccggatgcgg
    20101 cgcgagcgcc ttatccggcc tacaggtcgg caa
//
LOCUS       DAZUGK010000077        20091 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZUGK010000077 DAZUGK010000000
VERSION     DAZUGK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.077"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..221
                     /locus_tag="ACHY5W_002388"
     CDS             <1..221
                     /locus_tag="ACHY5W_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4447275.1"
                     /translation="YCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQK
                     AIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            432..755
                     /gene="ybjQ"
                     /locus_tag="ACHY5W_002389"
     CDS             432..755
                     /gene="ybjQ"
                     /locus_tag="ACHY5W_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4447276.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            752..1582
                     /gene="amiD"
                     /locus_tag="ACHY5W_002390"
     CDS             752..1582
                     /gene="amiD"
                     /locus_tag="ACHY5W_002390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFS4447277.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDNSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1579..2592)
                     /gene="ybjS"
                     /locus_tag="ACHY5W_002391"
     CDS             complement(1579..2592)
                     /gene="ybjS"
                     /locus_tag="ACHY5W_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4447278.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGSKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2691..4121)
                     /gene="ybjT"
                     /locus_tag="ACHY5W_002392"
     CDS             complement(2691..4121)
                     /gene="ybjT"
                     /locus_tag="ACHY5W_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFS4447279.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPILLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDSAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4132..5133)
                     /gene="ltaE"
                     /locus_tag="ACHY5W_002393"
     CDS             complement(4132..5133)
                     /gene="ltaE"
                     /locus_tag="ACHY5W_002393"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFS4447280.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHIDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5170..6888)
                     /gene="poxB"
                     /locus_tag="ACHY5W_002394"
     CDS             complement(5170..6888)
                     /gene="poxB"
                     /locus_tag="ACHY5W_002394"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFS4447281.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7021..7989)
                     /gene="hcr"
                     /locus_tag="ACHY5W_002395"
     CDS             complement(7021..7989)
                     /gene="hcr"
                     /locus_tag="ACHY5W_002395"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFS4447282.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLASVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVEEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8001..9653)
                     /gene="hcp"
                     /locus_tag="ACHY5W_002396"
                     /gene_synonym="priS"
     CDS             complement(8001..9653)
                     /gene="hcp"
                     /locus_tag="ACHY5W_002396"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFS4447283.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREVLKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9797..10696)
                     /gene="lysO"
                     /locus_tag="ACHY5W_002397"
     CDS             complement(9797..10696)
                     /gene="lysO"
                     /locus_tag="ACHY5W_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFS4447284.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11154..11849)
                     /gene="aqpZ"
                     /locus_tag="ACHY5W_002398"
     CDS             complement(11154..11849)
                     /gene="aqpZ"
                     /locus_tag="ACHY5W_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFS4447285.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            12275..13933
                     /gene="ybjD"
                     /locus_tag="ACHY5W_002399"
     CDS             12275..13933
                     /gene="ybjD"
                     /locus_tag="ACHY5W_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS4447286.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(13930..14886)
                     /gene="ybjX"
                     /locus_tag="ACHY5W_002400"
     CDS             complement(13930..14886)
                     /gene="ybjX"
                     /locus_tag="ACHY5W_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFS4447287.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15037..16152
                     /gene="macA"
                     /locus_tag="ACHY5W_002401"
     CDS             15037..16152
                     /gene="macA"
                     /locus_tag="ACHY5W_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFS4447288.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKNNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16149..18095
                     /gene="macB"
                     /locus_tag="ACHY5W_002402"
     CDS             16149..18095
                     /gene="macB"
                     /locus_tag="ACHY5W_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFS4447289.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPTIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18168..18392)
                     /gene="cspD"
                     /locus_tag="ACHY5W_002403"
     CDS             complement(18168..18392)
                     /gene="cspD"
                     /locus_tag="ACHY5W_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFS4447290.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18542..18616
                     /gene="yljB"
                     /locus_tag="ACHY5W_002404"
     CDS             18542..18616
                     /gene="yljB"
                     /locus_tag="ACHY5W_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFS4447291.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            18797..20020
                     /locus_tag="ACHY5W_002405"
     CDS             18797..20020
                     /locus_tag="ACHY5W_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4447292.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRAGTGRQISPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFLTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLDV
                     LAGNHENIVMLKVAK"
BASE COUNT         4949 a         5118 c         5330 g         4694 t
ORIGIN      
        1 aatattgtat taaactatta agcgctcagg atcgggcgtt tatatacgaa tttgcaagaa
       61 tgatcttcgc ttatattaat gacaaaagca ttgagatctt attactttct tgttttgcgg
      121 cggcggacca aaaagcaatc cagcggtata tacctcaggc gcaagatggg gaagacttta
      181 ggaatcattt acaatataag ttgactcttc ccactcatta atgttgccgg cacaacataa
      241 tagggcaggt aagcggtgct ccataatgaa accaatatcc ggttacgaaa cagataattg
      301 tacaggctta tgtagctgtt tttgacacaa tgagatgatt tcctaaactg atcaaaacct
      361 tctttccaac atggcatatc agtccgataa tgctttagag atcgaaggct taacaaacaa
      421 ggagatcgat catgcaattt tccacaactc caactctgga aggccagacc atcgttgaat
      481 attgcggtgt ggtgactggc gaagcgattt taggcgcgaa tattttccgt gatttctttg
      541 ccggtatccg cgatatcgtt ggcggacgct ccggtgccta cgaaaaagag ctacgtaaag
      601 cgcgggagat cgcctttgag gagcttggct cccaggcgcg ggcgctgggg gccgatgccg
      661 tagtcggtat tgatatcgac tacgaaacgg tcgggcaaaa cggcagtatg ctgatggtta
      721 gcgtcagcgg tacggcggtg aaaacgcgtc gatgagaaga gtcttctggc tgatcgctgt
      781 cgctctgtta ctggcagggt gcgcaggcga aaaaggcatt gtcgagaaag agggatatca
      841 gcttgatacc cgacgccagg cgcaggcggc gtatccgcgc attaaagtgc tggtgatcca
      901 ctacaccgca gatgattttg ataactcact ggcgacactg accgataagc aggtcagttc
      961 gcattatctg gtccctgcgg tgccaccgcg atacaacggt aaaccgcgca tctggcaact
     1021 ggtgccggaa caagaactgg cctggcatgc ggggattagc gcctggcgcg gggcaactcg
     1081 ccttaacgac acctctattg gcattgagct ggaaaaccgt ggctggcaga aatcggcggg
     1141 agtgaaatat tttgccccgt ttgaaccggc acagattcag gcgcttattc cgctggcgaa
     1201 agatattatt gcccgttatc acatcaagcc ggaaaacgta gtggcacatg cggatattgc
     1261 gccgcagcgc aaagacgatc cggggccatt atttccctgg cagcaactgg cgcagcaggg
     1321 gattggtgcc tggccggatg cgcagcgggt gaacttttac cttgccgggc gcgcgccgca
     1381 cacaccggta gataccgcgt cattgctgga gcttttggcg cgctacggtt atgacgttaa
     1441 acctgatatg acaccgcgcg agcagcggcg cgtgattatg gcgttccaga tgcatttccg
     1501 cccgacgtta tacaacggcg aagcggatgc agaaactcag gcaattgccg aagcgttgct
     1561 ggagaaatac gggcaggatt agcgcggcag ttttccatgg tcgcgtagcc aggcggcagt
     1621 tttctcgata ccttcatcca gggtgatgac tggctgataa cctaactctt cctgcgcccg
     1681 cgtaatatcc agcgtaaagt caaaattaag cttcgagacg ccgtagtggg tcagcggcgg
     1741 ctcttttgct gacttgctgc ctaaacgctc catgctgcgg gcgatcatat ccagcatcgg
     1801 gtaaggaacc gaacgaatac gacagtcaat attcaactcg tcgatcagct tctgcacgat
     1861 gctgcgcagt gtgcgatgct cgccgttggt gatgttgtac acacgcccgg aaggtagctt
     1921 atcgcaggct tcctggcttg ccagccacat ggcgtgcact gcattttcat agtacgtcat
     1981 atcgaccagc gcacttccgc cgtgcggtaa cagaatgctg ccgtagtggt gcatcatatg
     2041 cgccagacgg gggataaaga ctttatcgtg cggtccgaac agactttgtg ggcgcagaat
     2101 agtaaagcgc gtttgtggat tcgcctgcga aagcatattg atcacttctt cgctggctgc
     2161 tttgctgcgg gcaaactcgt tggcgaagcg gtgagggcga aaatcttctt taatatcgcg
     2221 atggtggtga taatcgaagt acagggaggg ggaagagata tgaataaagt tacgcacacc
     2281 ccaggcgaca gcccattcac ccaggcggcg agtggcgcga acgttagcca gatcgaaagc
     2341 ctgttgtgtc ccccagggcg aggtaaagct ggagcagtgc cacagcgtat caatgcccgc
     2401 gagcatcact ttagcttgtg atgagaccag ctcggtcaga tccgccggaa caaactctgc
     2461 gcccattttt tccagcaatt tacccattgc ctcgttgcga ccggtcgctc gcacgctgat
     2521 gcctttctgg cataaaaact ctaccgcgtt tcgacctaag ccgctggtgg cgccggtaac
     2581 cagtaccttc atatcaatcc actgttgttg agaaaatgac gtgcgcattc ttccgtgatt
     2641 tcccccatga tgcaatggga aacatgaaag aataacgcag gttttgtcga ttaatctgtg
     2701 ctttgttctg ccagtctggc gatttgtttt gccattccgc gaaaaataaa cagatgcgcg
     2761 gggatcatca ataaccagta aaacagcccc ggcataccgt gcggatgcca gaaagcgcgg
     2821 acatcgatag tacgatagtc gcctttatct tccagggtaa aacacagtcg tcccagcccc
     2881 ggtgctttca tgccaaataa caacgaaagt tgctttttcg gttcaacgac aatgactttc
     2941 cagctatcca ccgcatcgcc agtttgcaaa tattcgcgct ccggacggcc tttcgctaat
     3001 ttatgaccga tcgcacggtc catcaacgcc cgtgtctgcc acaaaatatt gccaaagaaa
     3061 taacgctctt taccgccgat ttggttcact acctgccata aagccgcaag gctggcggac
     3121 gttttaacgg taaaccccgc ctgtttggcg aaataaccgt actcaggtcg ccagcgggca
     3181 aaggcctgag cgtcgtagcc ccagtcgctg gagttgacca gtttttcttc ctctttcaac
     3241 gtgctacgta ccgcgtcatc gaaagcgatc agccgttgtg ggatgagcgc acgtagcgcg
     3301 gagtcatccg ccagcagatc gtgtttcagc ccctgaatca acgccctggc ggtggtgggc
     3361 ggtacggaag taatcacatt aagaaaccac accgaaatcc agcgagtggg gaggaggatg
     3421 gggatcaacc agcggcgctt accgctcacc gccataaaat gttcaaactg ttgctgataa
     3481 ctgagcacct ctggtccggc ggcttcgaag atgcggtgtt cgttggctgg atggtctaac
     3541 agcgccacca gatagtgcag caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc
     3601 cagcgtggcg gcgttaacac tggcaggttg tagaccatat cgcgcatgac ctcgaacgcc
     3661 gctgaacctg cgccaacgat aattccggcc cgcagttcgg tcactggtac attcgcttca
     3721 cgcagaatgt ccgccgtagc ctgacgagca cgcagatgat ccgactgctc atgtggcggg
     3781 gcctgcaacg aactaagaaa gattaattgc ttaactggta cttcacgtag cgcatcgcgg
     3841 acgttgagag ccacctggcg ctcctgagcg ataaaatcgc cgccttcgcc catgctgtgc
     3901 accagaaaat agaccgtatc gatatcctgc aacagggccg gaaggttatc cggccagctg
     3961 agatcgactt tatggcaact gacgtttgcc agttgcagct ttgcaagcct gtcgacatga
     4021 cgtgccgccg ccaggatctg atgtccttgc tggctgagtg tgcgcaccag atgctgacca
     4081 atgtagccac tggcaccgag aactaaaatg cgttgcggca cgtctctctc cttaacgcgc
     4141 caggaatgcg cgccagtggg cggcgacttc cgccagttgt tcgcgggaga cgtcaagatg
     4201 cgtcaccagg cggacaatcg gcgaggcgtt aatcagcacg tttctcgctt tcatgtattc
     4261 gcctaacgcg gcagcatttt cttccccgac gcgaacaaac agcatattag tgtcctgacg
     4321 catcacatcc gcgcctgctt cacgcagctg ctccgccatc caggcagcgt tgtcgtggtc
     4381 ttcctgcaag cgcgcaacat tatttttcag ggcatatatc ccggcggcag ccagaatgcc
     4441 ggactggcgc atcccgccac cggccatttt ccgccagcgg atggcacgtt taatgtaatc
     4501 acgattaccg acgagtaatg aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt
     4561 gaacgaatca caatattgcg tgatctcttt cagttcgcag ccgtaagcca ccacggcatt
     4621 aaagatgcgc gcaccgtcaa tatgcagcgc cagatttcgc tcgcgggtaa attcccatgc
     4681 ttctttaagg tattcacgcg gcagcacttt gccgttgtgg gtgttttcca gactgagtaa
     4741 tttggtgcgg gcgaaatgga tatcgtcggg tttgattttc atcgccactt tatccagcgg
     4801 tagcgtgccg tcggcagccg cgtcgatggg ttgcggttga atactgccca gcaccgccgc
     4861 gccaccggct tcaaacagat agttatgcgc ggcctgaccg acaatatact cttcgccacg
     4921 ttcgcagtga ctgagcagag cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc
     4981 ggcttcttta ccggaaagct ctgctgcata gtcctgcaaa gcattaacgg tagggtcgtc
     5041 tccgtaaacg tcgtccccaa ccggggcggc catcatcgct tcgagcatgg cgcggctcgg
     5101 tcgggttacg gtatcactgc gtaaatcaat catggcatgt ccttattatg acgggaaatg
     5161 ccaccctttt taccttagcc agttcgtttt cgccagttcg atcacttcat caccgcgtcc
     5221 gctgatgatt gcgcgcaaca tatacaagct aaatcctttg gcctgttcga gtttgatctg
     5281 cggtggaatg gctaactctt ctttggcgac caccacatcc accaataccg gaccgtcgat
     5341 ggagaaggcg cgttgcaggg cttcatcaac ttcagacgct ttttctacac ggatacccgt
     5401 aatgccgcat gcttcggcaa tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt
     5461 caggtagcct ccggctttca tctccatcgc cacaaagccc agcacgctgt tattaaagac
     5521 gataattttc accggcagtt tcatctgcac taccgagagg aaatcgccca tcaacatgct
     5581 aaaaccgcca tcgccgcaca tggcgaccac ctgacgttcc ggctcggttg cctgcgcgcc
     5641 cagcgcctgc ggcatggcgt tagccatcga accgtggtta aacgaaccta acaggcgacg
     5701 cttgccgttc attttcagat aacgcgctgc ccacaccgtt ggcgtaccaa cgtcacaggt
     5761 gaaaatggcg tcatcggcgg caaaatgact aatttgctgc gccagatatt gcgggtgaat
     5821 ggctttctcg ctcggtttag ctaaatcgtc cagccctttg cgggcatcgc ggtaatcttc
     5881 cagcgcttta tccagaaatt tgcgatcggc tttttcttcc accagtggca gcaatgcacg
     5941 cagggttgat ttgatatcgc cgaccagtgc catatccacc ttgctgtgag cgccgatgct
     6001 ggctggattg atatcaatct gaataatttt ggcatcggtc gggtagaacg cacggtaggg
     6061 gaactgagta ccgagtagca ctaacgtgtc ggcgttcatc atggtatgga aacctgacga
     6121 gaatccgatt aacccggtca ttccaacatc atacggatta tcgtattcga catgctcttt
     6181 accgcgtagg gcatgaacaa taggtgcttt aattttcccg gcaaactcaa ctaactcttt
     6241 atgcgccccc gcgcagccgc tgccacacat cagagcgata ttgctggaat aacgcagcag
     6301 ttgcgccagt ttgcgtaact cttcttcttc cggcgtcaca actggctgtg gcgcatgata
     6361 ccagtgcgtg gttgcccctt ctggcgcagg ttttaacgcc acgtcacccg gtaacacgac
     6421 aacggaaacg ccacggttaa gcaccgcttt gcgcatagca attgccagca cttgtgggat
     6481 ctgctccggg ctggaaacca gctcgcaata gtgactacat tcgcggaata gctcttgtgg
     6541 gtgagtttcc tggaaatagc cgctgcctat ttcgctggag ggaatatgag cggcaatcgc
     6601 cagtaccgga acgtgattgc ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc
     6661 ggggccgcac gatccggcac agaccgccag ttctcctgaa agttgtgctt cagcgccagc
     6721 ggcaaacgcc gccacttctt catggcgggt ggacatccac tcgatggtgc ccatgcgatt
     6781 aagactgtcg ctaagaccgt tcagagagtc gcccgtgact ccccagatgc gtttcacgcc
     6841 tgccgattcg agtgttttgg cgatataagc tgcaaccgtt tgtttcatgg ttctccatct
     6901 cctgaatgtg ataacggtaa caagtttagt tcatctgacg gagggggaag ggatgggaga
     6961 aaaaggaggc actaacggtt aaatagcccg atgaaatgaa tatcatcggg cataaggcga
     7021 ttatgcgaga accaaatccc cctgcggatg gcaggagcag gccagtacgt aaccttcagc
     7081 gatttcggcg tcggtcagcg tcattgtgct gctcaccgta tattcaccgg aaaccacttt
     7141 tgtcttacag cagccgcaaa cacccgcacg gcaggcagcg acaaccggaa cgttattgct
     7201 ttccagcgcc tccagtagcg tggtgccaac cggagcgtaa aattctcgtg ccggttgcag
     7261 tttggtgaat ttcagaccgc tggtcgccgc ttcctctact ggagtgaaga atttctcttt
     7321 aaagaaacgc gtcacgccga gcgctttcac ttcctgttct acccaatcca tatacggtgc
     7381 tggaccacaa gtcatcacgg tacgtgaggc taaatcagga acgcttgcca gtagttcgcg
     7441 agtgagacga ccagcgataa agccttcggt aacgttattt tccgccacca gcgttaccgg
     7501 atagttacgc cactcatcgg cgaaaataac atcctgcggc gtacgcacgt tgtagatcac
     7561 ccgcacatcg gcctgtggac ggttcttcgc cagccagcga cgcatcgaca taatcggcgt
     7621 gacgccgcag cctgccgcca gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa
     7681 ttcccccatc gcgtctgaaa gccagagata atcaccgcgt tttacatcgc gggtcagcca
     7741 ctgggagccg acaccgtcat caatccgccg cacggtcagg gtgatgtatt cactcactcc
     7801 tggcgtggag gaaatagtgt aagcacgcag cgtttccgct gagttacgca cgctgaccag
     7861 tgcatattgc ccggcgcgat atgggtagta atcgtggcaa atcagggaaa tcgtccacac
     7921 atccggcgtt tcttgcttaa tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg
     7981 catcgtcatc tgctaactcc ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg
     8041 gtgatagaac gcaggccgaa tttctcgttc agcaccgcca gcaggtctgg tgtcaggaaa
     8101 ccaggtgcag tcgggccggt gacgatattt ttcacgccca gagaaagcag cgtcagcaga
     8161 atgacaatcg ctttctgttc aaaccaggag agcaccagcg acagcggcag atcgttcaca
     8221 ccacagccca gtttctctgc cagagtgaca gccagaataa tcgctgagta agcatcgtta
     8281 cattgacctg catctaccag acgcggcaga ccttcgatat cgccaaactc aagtttgtta
     8341 aagcgatatt taccacaggc gagggtcagg atcaggcagt catccggcac gctggtggcg
     8401 aaatcggtga agtagtgacg ttcgccgcgt gcgccgtcac agccgccaag caggaagata
     8461 tgacgcagtt tttcacggct caccagatca atcagcgtat ccgctgcgcc aagcagcgtc
     8521 tggcggccaa agcccacggt gatcaggtgc ggaatttcgc tgtatgggaa gcctgccatt
     8581 tgttgcgcct gggcgatgac cgcagaaaaa tcttcgccat ccagatgacg cacacccggc
     8641 cagccaacaa tgctgcgggt ccagatacga tcgtcataag cgcctacggt tgggtcgatg
     8701 atgcagttcg aggtcatcac gatggggcca gggaaacgag cgaactccac ttgctgattc
     8761 tgccagccgc tgccgtagtt accgaccaga tgcttgaatt tacgcagctc cgggtagccg
     8821 tgtgcaggca gcatttcgcc gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc
     8881 agcaggttgt agagatcttt gagatcgtga ccggaaatca ggatgcattt acccgccgtc
     8941 gctttgacgt tgacctgagt tggggtcggg tgaccgtatt taccggtttc acctgcgtcc
     9001 agaatgctca tcactttgaa gttcatctgg ccgatttcca tagaacactc aagaagcgcg
     9061 ttcatatcgg caggccaggt ccccagccac gccatgattt tatggtactg ggcataaata
     9121 tcattgtcgt attgaccgag aacgtgtgcg tgttccatat aggccgccgc acctttcagg
     9181 ccatacaggc acagcagacg caggccgaga atgttttcgc caatcgccgc tttatctttg
     9241 ttaggggtaa attctgctgc ctgacgttgc agctcgccga gatcgtcgct caccagttgc
     9301 aggtcagcca tcgggttatc gacgcgcgcg ttggcatcta cagccaggca ttgtgctttc
     9361 agcacctcgc gcagggcaat cgcttcacga gcgtagccga ctatacgcgg agaatcgaag
     9421 ttaacgttgg tcagggttga gaaaaatgcg cgtggcgcaa agctgtcgac atcgtggttg
     9481 atgatgccgt attcacgcgc ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg
     9541 agtaaatcct gaaggtcaga agtttccgcc gttttaccac acatcccctg cgcgtatgag
     9601 cagccgtttc ctgccggagt acggatagtt tgttcacatt gcacacaaaa catgatcaca
     9661 ccttttaaag ttatatttaa tatacatgtt taaggttaag acgcttaacg cggggataaa
     9721 agggattttt catgcaactt taagggagat tgatttagcg caattttggc ggcagggatc
     9781 taccgccaga gaggtattac gcggagaaaa aggcgatgag gatcggcact aacaggctaa
     9841 gaataaaacc gtgaacaatt gccgccggga ccatatccag cccgccagta cgttgaagaa
     9901 cgggcagggt gaaatccatc gacgtcgcac cgcataagcc cagtgcggta gagcggctgc
     9961 ggcgaatcag cccagggatc aacataattg caatcagctc acgggccaga tcattaaaaa
    10021 acgccgcgct cccgattacc ggaccaaaag attcggtcaa taaaataccg gaaagagaat
    10081 accagccgaa accagaggcc attgccagcg cggtattgat gggtagatca agaataaagg
    10141 cgttaattaa accaccaatt aatgaactgg caaccaccac cacggcgaca atcattcccc
    10201 ggcgattaag gacaatctgc tttaaggtca tgccattatt gcgcaactga ataccgacga
    10261 ggaaaagtag cagaattaag gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca
    10321 gtccactcag gccaatggca aaaccaatca ctactacgcc gcacagtttt agcgactcaa
    10381 gcgccatcgc aatacgcgac gggagttttt cttgctgatg gtgattgcgc catggcaggc
    10441 ctcgctccag ccacatcagg gcggcaatat tacacagtaa aataacggta atactgacag
    10501 cagaataatg cagaatcgcc aacaggttac tggcgaggtt atcgagaaac gccagactga
    10561 tacccataaa aaagagaata aggtaaacca tccagcttaa tagctgatta ataactctta
    10621 atgcagcttt ttggcgaagc ggaatgaggt aacccacaat caggggaacc agaatgatta
    10681 acagcccaga aaacatgaaa acccagtcct tgcaaagaaa gaagtcgaaa tgcgcgatga
    10741 cacactactg aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca
    10801 gagggggatt tttaattcta tatgagtgtt tagcattgtt gcgctccttt catgtgcaat
    10861 gccatataaa gccactggcg cagggagcgc gcagggggcg cccagtcgcc gccgccccct
    10921 gcacccccgg gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt
    10981 cggcaaccgg gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat
    11041 gtgggctgcc ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc
    11101 ggtttgtaat gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatta
    11161 cgcttttcca gcagagtccg gtaaatcaga ccaccgataa tgccaccgac aattggcacc
    11221 acccagaaga accacagttg ttctaatgcc cagccgccct ggaagatggc aaccgcggtg
    11281 ctgcgcgccg ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt
    11341 aaggccagac caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg
    11401 tggatcacta acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg
    11461 gaataaccgc ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg
    11521 tcaaaacccg ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg
    11581 acaacctggg caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat
    11641 aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc
    11701 atcgtcagaa cggtcaaacc gaatgccagc gccacgccgg caaaaccaat gcctaattcc
    11761 gggaagcctg cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca
    11821 aaacattcag ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
    11881 aaatatggtc gttttatagg gtcgtcgtaa aaaagtgacg acggaaataa tgcgcggcta
    11941 ttttaaaaac gaaggcgagt cattcaccag ataaataaat ccagtaaatt tgatttaggg
    12001 caacagcggg ttgccccata tagtcatttg tctgattgac agtgtagtgc aagcaaaaga
    12061 tttaatcctt taggcgtaat aaaaaataat ttatcatgct aattatttga ttttgttgtt
    12121 tttgcagact tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt
    12181 tgccatgctt aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag
    12241 cgtaaaggat aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt
    12301 gggttttcgc ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg
    12361 ggagaacgcg tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga
    12421 atcagatctc taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg
    12481 gcgagaacat catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg
    12541 ggttcgccgt tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg
    12601 tattttttat cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag
    12661 ttttctcgat aaagacggac atccgattga tgttgaggat attaacgatc aggcacgcca
    12721 tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg
    12781 taacggcacg gtgccaaatg tccctaatgt ggaagtcacc gcgcgccagc tcgatttcct
    12841 cgcccgtgag ttgtcctcac atccgcaaaa tctctctgat ggacagattc gtcagggact
    12901 ttccgcaatg gtgcagctgc ttgagcatta tttctctgag cagggggctg gacaggcgcg
    12961 atatcgttta atgcggcggc gggccagcaa tgagcaacga agctggcgct atctggacat
    13021 catcaaccgg atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct
    13081 gtttgctact ttattgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt
    13141 gttgctgatc gaagatccag aaacgcgttt acaccccatt atgctttcag ttgcctggca
    13201 tctgttgaat cttttgcctt tacaacgtat tgctaccacc aactctggcg agctgctttc
    13261 gttaactcca gtggaacatg tttgccgcct ggtgcgtgaa tcttctcgtg ttgccgcctg
    13321 gcgtctgggg ccaagtggct tgagcaccga agacagccgc cgcatctctt ttcacattcg
    13381 ttttaatcgt ccgtcatcgc tgtttgctcg ctgctggttg ctggtggaag gggaaacgga
    13441 aacctgggtt atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat
    13501 caaggtcatt gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat
    13561 ggggattgaa tggcatgtgc tggtcgatgg cgatgaagca gggaagaaat atgccgctac
    13621 ggtacgcagc ctgttgaata atgatcggga agccgaacga gaacatttaa cggcgttacc
    13681 ggcgctggat atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt
    13741 ggcgcaaatc ccggaaaatg taccgatgaa tctgcgcaaa attatctcga aagcgatcca
    13801 tcgctcttcc aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt
    13861 ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg
    13921 tcgcgcggat taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc
    13981 atagcgccga cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc
    14041 tatctgtgct ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca
    14101 gaaagcgttg taatcggcat ggatcttgcc ttctttatca cgataacgca ggctgcggta
    14161 aatatgcgtt tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag
    14221 acaggccgct tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt
    14281 ctggatttcc tgatgtggga tttcccattt tgcgccttgc agtcctccaa taaacatcgt
    14341 tcttttcccc tgatattcac acagggtaaa agtgagctct gccagaggaa taccttcgct
    14401 gttgcggaac aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc
    14461 aagcgtgaac tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt
    14521 caaatataag ctgaattctt ccgccgacat acacccgcgg agtaacgcat aatggtaacg
    14581 taacgcctcc agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg
    14641 tacaggcagt cgcggctggc gcgttaacaa cacattgaga ttaggccagt gtgaaagttc
    14701 gttcatccac tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg
    14761 aaaactacgg cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa
    14821 aagtgacagg ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat
    14881 attcatgaaa tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac
    14941 atcaatagtg tgattactaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa
    15001 tgacccggat tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag
    15061 aagcgttacg ttattgcgct ggtgatagtc atcgccgggc tgattacgtt atggagaatt
    15121 cttaacgcac ccgtgccgac ttatcagacg ctgattgtgc gccccggtga tttacagcaa
    15181 agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc
    15241 agcggtcagt tgaaaacgct gtcggtggcg attggcgata aagtaaaaaa agaccagctt
    15301 ttaggggtta ttgatcctga acaggctgaa aaccagatca aggaggtcga agcaacgctg
    15361 atggagctac gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg
    15421 tattcccgtc agcaacgtct ggcacaaacg caggcggttt cacagcagga tctcgacacc
    15481 gccgcgacgg agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag
    15541 cgcaatcagg cttctctcga tacggctaaa aacaatctcg attacactcg tatcgttgcc
    15601 ccgatggccg gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca
    15661 caacaagcac cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag
    15721 gtttctgaag cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtactt
    15781 ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag
    15841 gttaacgacg ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg
    15901 cggctggata tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg
    15961 atccctctgt cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg
    16021 cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt
    16081 gagattgtca aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga
    16141 gctgcgcaat gacacccttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg
    16201 atgagcaggt tgaggtgctg aagggcatca ccctcgatat ttatgccggt gagatggtag
    16261 cgattgttgg cgcttcgggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg
    16321 ataaggccac cagcggcacc tatcgcgtcg ccggtcagga tgttgccaca ctggacgccg
    16381 atgcgctggc gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc
    16441 tttcgcattt aaccgccgag cagaacgttg aagtacccgc cgtctatgcc ggtcttgagc
    16501 ggaaacagcg gctgcttcgc gcccaggagt tactgcaacg actggggctg gaagaccgta
    16561 cagagtatta tccggcacag ctttcgggtg gtcagcaaca gcgcgttagc atcgcgcggg
    16621 cattgatgaa cggcggtcag gtaattcttg ccgatgaacc gaccggcgca ctggacagcc
    16681 attcaggcga agaggtgatg gcgatcctgc atcagctgcg cgatcgcggg catacggtga
    16741 ttatcgtcac tcacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg
    16801 acggcgaaat tgtgcgcaat cctcccacca ttgaaaaagt gaatgttgct ggcgggacgg
    16861 aacctgttgt caacacggcg tctggctggc ggcagtttgt cagcggtttt aatgaggcgc
    16921 tgacgatggc atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg
    16981 ggattattat cggtattgcg tcggtggttt ccattgtcgt ggtgggcgac gccgccaaac
    17041 aaatggtgct ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga
    17101 aagattttgg cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg
    17161 ccatccaaaa acaaccgtgg gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc
    17221 tgcgttataa caatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta
    17281 atgtctatgg catgaccttc agtgaaggga acacctttaa tcaggagcag ctgaacggtc
    17341 gtgcgcaggt cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag
    17401 atgtggttgg cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg
    17461 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttatagca
    17521 cgatgtccgg gcgagttatg ggccagtcgt ggcttaactc cattactgtc agggtgaaag
    17581 aaggatttga cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
    17641 gaaagaagga tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca
    17701 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtt gtgggcggta
    17761 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
    17821 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac
    17881 tggtttgcct ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct
    17941 tgcagctttt cttacccggc tgggagattg gtttttcacc gttggcgctg ctgctggcgt
    18001 ttctctgctc gacggtcacc gggattttat ttggctggtt acccgcacga aatgcggcac
    18061 gactggatcc agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga
    18121 tcgggctggc attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc
    18181 gcttctactt cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca
    18241 tcaaactgaa cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa
    18301 taatgagcga aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca
    18361 ttgttgaacc acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac
    18421 aagtaagatg gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag
    18481 aaatacaatt tagagaatta gggcgagccg tcaagcattt gacaggggac aaggggcagg
    18541 tatgaatcaa aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga
    18601 tgccaacgat agatgatagt tatttatcag ttagtgttcg tgggtgttcg tcatggtgtg
    18661 ggcatcccat ttatttctat gattataaag gataatttcg tttttcttgt tcgcccttgt
    18721 tcgtcacagt tcgcccttgt acgatgcacg gtgtagtcaa tggtgtagtt aattttgatg
    18781 accggaaaca cgaaacatga gcagggcaat tcatagactt agcgacactc ttttacgaaa
    18841 attaagcgga tcaccaacca caaaaagcac cttttttaat gatggtggca accttagcgt
    18901 aaggcattca accaatggcc tgttaacctg gtatttcact tacagggccg gaactggtag
    18961 gcagatatca ccggaacgtc tgaggctggg caattatccc gatctgagcc tgaaagctgc
    19021 cagggaaaaa gcggcacagt gtcgcgcctg gctggctgag gggaaaaatc cgcgctatga
    19081 acttaatcat gctgtacagg acgcgttagc ccccgtgacg gttaaggaag cgctcactta
    19141 ctggcttgaa tcgtacgcaa aggaaaagcg cactgactac gaatcactga aaagccggat
    19201 caataaacac ataattagcc agattggcgc tatgccactg gaaaaatgcg agctacgcca
    19261 ctggctggcc tgttttgacc agcttgctaa gcgtaatccg gtatcagccg gatttttgct
    19321 acaggtatgc aaacaggcgc ttaagtactg ccgaaaacga cgatacgcaa tcagcaacgt
    19381 tcttgatgat atggttgtcg gtgatgttgg gaaaaaagca gaaataagcg agcgcgtact
    19441 aacaaacaag gagcttgggg aattactacg cgcccttgat gaaaaaattt acccaccgta
    19501 ctacagcgcc ctgattcgcc tcctgattgt cttcggatgt cgtaccactg agctaagacg
    19561 ctctgaggtc caggaatggg attttaaaga aatgctctgg accgtgccga aagaacacag
    19621 caaaacgaag gtagcaatat tcaggccaat accggaagcg atcttgccgt tcttaacgaa
    19681 gctggtggag cagaacaggc acacgggatt attgctcgga gaactgaaag ggcaatcatc
    19741 tgtatcagag tacggaagaa cggcacacag acgcattaat caagccccct ggacgttgca
    19801 cgacatccgg cacacgttta caacgatgct gaacgattta ggcgtggaac cgcacgttgt
    19861 ggagcagctt accgcgcacc agttgccagg aatgcaacga gtctataatc actcccgtta
    19921 tcttgatgca aaacgtgacg ctcttaatct atgggttgag cgtctcgatg tgctggctgg
    19981 aaatcatgaa aatattgtta tgctgaaggt ggccaaatga aattacctaa aagactctat
    20041 tatccgttac ctgaagcggc agaaaagtta ggctgtgcaa taaaagatct t
//
LOCUS       DAZUGK010000078        20000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZUGK010000078 DAZUGK010000000
VERSION     DAZUGK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.078"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(196..1008)
                     /locus_tag="ACHY5W_002406"
     CDS             complement(196..1008)
                     /locus_tag="ACHY5W_002406"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFS4447293.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKRS"
     gene            1270..2031
                     /gene="udp"
                     /locus_tag="ACHY5W_002407"
     CDS             1270..2031
                     /gene="udp"
                     /locus_tag="ACHY5W_002407"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFS4447294.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2172..3599
                     /gene="rmuC"
                     /locus_tag="ACHY5W_002408"
     CDS             2172..3599
                     /gene="rmuC"
                     /locus_tag="ACHY5W_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFS4447295.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3694..4449
                     /gene="ubiE"
                     /locus_tag="ACHY5W_002409"
     CDS             3694..4449
                     /gene="ubiE"
                     /locus_tag="ACHY5W_002409"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFS4447296.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4463..5068
                     /gene="ubiJ"
                     /locus_tag="ACHY5W_002410"
     CDS             4463..5068
                     /gene="ubiJ"
                     /locus_tag="ACHY5W_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFS4447297.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5065..6705
                     /gene="ubiB"
                     /locus_tag="ACHY5W_002411"
     CDS             5065..6705
                     /gene="ubiB"
                     /locus_tag="ACHY5W_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFS4447298.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6784..7053
                     /gene="tatA"
                     /locus_tag="ACHY5W_002412"
     CDS             6784..7053
                     /gene="tatA"
                     /locus_tag="ACHY5W_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFS4447299.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7057..7572
                     /gene="tatB"
                     /locus_tag="ACHY5W_002413"
     CDS             7057..7572
                     /gene="tatB"
                     /locus_tag="ACHY5W_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFS4447300.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPATAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7575..8351
                     /gene="tatC"
                     /locus_tag="ACHY5W_002414"
     CDS             7575..8351
                     /gene="tatC"
                     /locus_tag="ACHY5W_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFS4447301.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8393..9175
                     /gene="tatD"
                     /locus_tag="ACHY5W_002415"
     CDS             8393..9175
                     /gene="tatD"
                     /locus_tag="ACHY5W_002415"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFS4447302.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELQELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9172..9660)
                     /gene="rfaH"
                     /locus_tag="ACHY5W_002416"
     CDS             complement(9172..9660)
                     /gene="rfaH"
                     /locus_tag="ACHY5W_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFS4447303.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9827..11320
                     /gene="ubiD"
                     /locus_tag="ACHY5W_002417"
     CDS             9827..11320
                     /gene="ubiD"
                     /locus_tag="ACHY5W_002417"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFS4447304.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11366..12067
                     /gene="fre"
                     /locus_tag="ACHY5W_002418"
     CDS             11366..12067
                     /gene="fre"
                     /locus_tag="ACHY5W_002418"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFS4447305.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12259..13422)
                     /gene="fadA"
                     /locus_tag="ACHY5W_002419"
     CDS             complement(12259..13422)
                     /gene="fadA"
                     /locus_tag="ACHY5W_002419"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFS4447306.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13432..15621)
                     /gene="fadB"
                     /locus_tag="ACHY5W_002420"
     CDS             complement(13432..15621)
                     /gene="fadB"
                     /locus_tag="ACHY5W_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFS4447307.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPELRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     AFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15811..17142
                     /gene="pepQ"
                     /locus_tag="ACHY5W_002421"
     CDS             15811..17142
                     /gene="pepQ"
                     /locus_tag="ACHY5W_002421"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFS4447308.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDEPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWPAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17142..17756
                     /locus_tag="ACHY5W_002422"
     CDS             17142..17756
                     /locus_tag="ACHY5W_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFS4447309.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17795..19246
                     /gene="trkH"
                     /locus_tag="ACHY5W_002423"
     CDS             17795..19246
                     /gene="trkH"
                     /locus_tag="ACHY5W_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFS4447310.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19258..19803
                     /gene="hemG"
                     /locus_tag="ACHY5W_002424"
     CDS             19258..19803
                     /gene="hemG"
                     /locus_tag="ACHY5W_002424"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFS4447311.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         4629 a         5077 c         5493 g         4801 t
ORIGIN      
        1 tgggagtgga tggtaaatct ctaaggtgtc ttccattgaa gagactcatt tatgactatg
       61 acttgccgac tttcttaagg tcaaatagcg gagtcgtaat ctccaatcaa ttcatactga
      121 ttattttttg ggggacagag atggcagcaa aacgcactcg ttcactcacc atctcaaata
      181 tagactaaga ggaaactaac tccgcttccc accatactgc ttaaaccatt ccagcatacg
      241 ctgccagcca tcttctgcag atgcggcatg atagctcggg cgataatcag cgttgaatgc
      301 atgcccggcg tccgggtaca cgataatctc tgctttcgca ttagcagccc gcagcgcctg
      361 gcgcatggtt tcaacgctct cctgcggaat gctgttatcc tgaccaccat ataagccgag
      421 aatcggcgcg ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt
      481 gtcgcccgtc agtttgccgt accacgccac tgcggctttt agctgtggat tatgcgcggc
      541 atacagccag gtgatacgtc caccccagca gaatccggtg atcattaaac gatgaacatc
      601 gccgccgttg cgggaagccc aactggcgac atgatcgaga tcggccagca cctgcgagtc
      661 aggcactttt gctaccagac cgctaagcaa cgtggggata tcggcaaaat cattcggatc
      721 gccttcgcgg aagtaaagtt caggtgcgat agccagatac ccctccagcg ccagacggcg
      781 acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc tgcactacaa tgaccactgg
      841 cagtgggcca tcgctttgct ttggtctggc atggtaagca ggcatgttat ccccttgtga
      901 agggatagag gtgaagcccg ccacaatcgc gtcgtctggg gtctgaacga tggtcgaagc
      961 gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg tattctccgt
     1021 acccttataa aaatgttgcg caatgttaac tatagtcagc atgcaacaaa tcacattgcc
     1081 tgaatcggct catcttttat gcagtcctgc agaatgaaga gtgatttatg tgatttgcat
     1141 cacttttggt gggtaaattt atgcaacgca tttgcgtcat ggtgatgagt atcacgaaaa
     1201 aatgttaaac ccttcggtaa agtgtctttt tgcttcttct gactaaaccg attcacagag
     1261 gagttgtata tgtccaagtc tgatgttttt catctcggcc tcactaaaaa cgatttacaa
     1321 ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg
     1381 atggataagc cggttaagct ggcatctcac cgcgaattca ctacctggcg tgcagagctg
     1441 gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac ctctattgct
     1501 gttgaagagc tggcacagct gggcattcgc accttcctgc gtatcggtac aacgggcgct
     1561 attcagccgc atattaatgt gggtgatgtc ctggttacca cggcgtctgt ccgtctggat
     1621 ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg tcgctgattt cgaatgtacg
     1681 actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct
     1741 tcttctgata ccttctaccc aggtcaggaa cgttacgata cttactctgg tcgcgtagtt
     1801 cgtcacttta aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg
     1861 gaatctgcaa ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg
     1921 ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg ctgagacgat gaaacaaact
     1981 gaaagccaag cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta attctcttct
     2041 cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat
     2101 gcctttccaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt
     2161 caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg
     2221 caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg
     2281 aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc caaagcgagc
     2341 actggcgtgc agagtgcgag ttactcaata acgaagtgcg cagcctgcaa agtattaaca
     2401 cctctctgga ggccgatctg cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg
     2461 ctgacgataa aattcgccag atgattaaca gcgagcagcg cctcagtgag cagtttgaaa
     2521 acctcgccaa ccgtattttt gagcacagca atcgccgggt tgatgagcaa aaccgtcaga
     2581 gtctgaacag cctgttgtcg ccgctacgtg aacaactgga cggtttccgc cgtcaggttc
     2641 aggacagctt cggtaaagaa gcacaagaac gccataccct gacccacgaa attcgcaatc
     2701 tccagcaact caacgcgcaa atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag
     2761 gcgacaataa aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt
     2821 ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct
     2881 cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg aaaagatgtg gtgatcgacg
     2941 ccaaaatgac gctggtcgcc tatgaacgct attttaacgc cgaagacgac tacacccgcg
     3001 aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca
     3061 aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg tttattcccg
     3121 ttgaacccgc ttttttactg gcgcttgacc gccagccgga gctgatcacc gaagcgttga
     3181 aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgctgcgc actatcgcca
     3241 acctgtggcg ttatgagcat caaagccgca acgcccagca aatcgccgat cgtgccagca
     3301 agctgtacga caagatgcgt ttgttcgtcg atgacatgtc agcgattggt caaagtctcg
     3361 acaaagcgca ggataactat cggcaggcaa tgaaaaaact ctcttcaggt cgcggaaatg
     3421 tgctggcgca ggcagaagcg tttcgcggtt taggagtaga aattaaacgc gagattaatc
     3481 cggatttggc tgaacaggcg gtgagccagg atgaagagta tcgacttcgg tcggttccgg
     3541 agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc
     3601 ccattgggag tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact
     3661 tctggaacaa ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg
     3721 cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc
     3781 cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcatttgg tattcatcgt
     3841 ttgtggaaac gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat
     3901 ctggctggtg gcaccggcga cctgacagcg aaattctccc gcctggtcgg agaaactggc
     3961 aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt
     4021 aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc gctgccgttc
     4081 ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa
     4141 gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt
     4201 gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat
     4261 gtgctgccgc gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca
     4321 gaatccatcc gtatgcatcc cgatcaggat accctgaaag ccatgatgca ggatgccgga
     4381 ttcgaaagtg tcgactacta caatctgacg gcaggggttg tggcgctgca tcgtggttat
     4441 aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag
     4501 tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt
     4561 gggcaaagtg ctgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag
     4621 cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc
     4681 ctacgccagt gtgttgccga aacttcgcga tcgccagcag cttgccgccc tgattcgcag
     4741 tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg cgctggcaga
     4801 tctggcagag ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga
     4861 aggaatcagc aaagccatgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca
     4921 gcaacgttat gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga
     4981 agtggcctgg tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa
     5041 acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc
     5101 attcgcactt ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg
     5161 ccgctacggc tatggcgata ctcattattc tggatgccaa atcggcataa agacaaacct
     5221 ttaggtgagc gactacgact ggccctgcaa gaactggggc cggtatggat caagttcggg
     5281 caaatgttat caacccgccg cgatcttttt ccgccgcata ttgccgatca gctggcgtta
     5341 ttgcaggaca aagtcgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca
     5401 atgggcggct tgccggtaga agcgtggttt gacgattttg aaatcaagcc actggcttct
     5461 gcttctatcg cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt
     5521 aaagtcatcc gcccggatat tttgccggtc attaaagcag acctgaaact tatctaccgt
     5581 ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc gtctacgccc aaccgaagtg
     5641 gtgcgcgagt acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaac
     5701 gctattcagc ttcggcgcaa ttttgaagac agcccgatgc tctacatccc ggaagtttac
     5761 cctgactatt gtagtgaagg gatgatggtg atggagcgca tttacggcat tccggtgtct
     5821 gatgttgcgg cgctggagaa aaacggcact aacatgaaat tgctggcgga acgcggcgtg
     5881 caggtgttct tcactcaggt ctttcgcgac agctttttcc atgccgatat gcaccctggc
     5941 aacatcttcg taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg
     6001 attgttggct cgctaaacaa agaagataaa cgctatctgg cagaaaactt tatcgccttc
     6061 tttaatcgcg actatcgcaa agtagcagag ctacacgtcg attctggctg ggtgccacca
     6121 gataccaacg ttgaagagtt cgaatttgct attcgtacgg tctgtgaacc tatctttgag
     6181 aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt
     6241 cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctacgtc
     6301 gaaggggtag gacgccagct ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc
     6361 ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc tggtgagagc atttaaagaa
     6421 aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg aattggttta cgacagtttg
     6481 cgccagggca agtatttaca gcacagtgtt gataagattg cccgcgagct tcagtcaaat
     6541 catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt
     6601 ggcacattct tgttggtcag ccgacctgag tgggggctga tgcccggctg gttaatggca
     6661 ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca
     6721 aggcgggccg tgtaacgtat aatgcggctt tgtttaatca tcatctacca cagaggaaca
     6781 tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt
     6841 tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt
     6901 aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagtcagga tgctgatttt
     6961 actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa
     7021 gacgcgaagc gccacgataa agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa
     7081 ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg
     7141 gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac
     7201 gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag
     7261 gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaact acgccaggcc
     7321 gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg
     7381 cacaccatcc ataacccggt ggtgaaagat aatgaagctg cgcatgaagg cgtaacgcct
     7441 gccactgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg
     7501 gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt
     7561 gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctgattgagc
     7621 tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt gatattcctg tgtctggtct
     7681 atttcgccaa tgacatctat cacctggtat ccgcgccatt gatcaagcag ttgccgcaag
     7741 gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct
     7801 ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg
     7861 ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc
     7921 tgctgtttta tatcggcatg gcgttcgcct actttgtggt ctttccgctg gcatttggct
     7981 tccttgccaa taccgcgccg gaaggggtgc aggtatccac cgacatcgcc agctatttaa
     8041 gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg
     8101 tgctgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg
     8161 tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa
     8221 cgctgttggc gatcccgatg tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt
     8281 acgttggtaa agggcgaaat cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa
     8341 ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga
     8401 tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg
     8461 cgcttttgac gcgggagtta atgggctact catcaccggc actaacctgc gtgaaagcca
     8521 gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc
     8581 tcacgacagc agccagtggc aagctgcgac tgaagaagcg attattgagc tggccgcgca
     8641 gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc
     8701 ggaagagcaa gaacgcgctt ttgttgctca gctacgcatt gccgcagaat taaacatgcc
     8761 ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct
     8821 ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc
     8881 gtgcgtggcg catggaattt atatcggcat taccggttgg gtttgcgatg aacgacgcgg
     8941 actggagctg caggaacttt tgccgttgat tccggcggaa aaattgctga tcgaaactga
     9001 tgcgccgtat ctgctcccgc gcgatctcac gccaaagcca tcatcccggc ggaacgagcc
     9061 agcccatctg ccccatattt tgcaacgtat tgctcactgg cgtggagaag atgccgcatg
     9121 gctggctgcc accacggatg ccaatgtcaa aacactgttt gggattgcgt tttagagttt
     9181 gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat taagcaatag
     9241 catggagcga gcctcaccat cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc
     9301 ttcggtaata atcaccttat cacccggata aggggttgcc ggatcgacaa tgtctttcgg
     9361 tttatatacc gatagctgat gaatcaccgc cgatgggact atcgctggcg acgcgccaaa
     9421 gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg
     9481 atcaaattcc acaaacaggt agttggggaa caatggctca ctgactgcag tacgtttgcc
     9541 acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag
     9601 gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc aggattgcat
     9661 aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg ctaagttaat
     9721 tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa atttacagca
     9781 tcgcaaagat gaacgccgta taatgggcgc agattaagag gctacaatgg acgccatgaa
     9841 atataacgat ttacgcgact tcttgacgct gcttgaacag cagggtgagc taaaacgtat
     9901 cacgctcccg gtggatccgc atctggaaat cactgaaatt gctgaccgca ctttgcgtgc
     9961 cggtgggcct gctctgttat tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa
    10021 tctgttcggt acgccaaagc gcgtggcgat gggcatgggg caggaagatg tttcggcgct
    10081 gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg gagccgccaa aaggtttccg
    10141 cgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga caaagcggct
    10201 gcgcggtgcg ccttgccaac aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat
    10261 tcccattatg acctgctggc cggaagatgc cgcgccgctg attacctggg ggctgacagt
    10321 gacgcgcggc ccacataaag agcggcagaa tctgggcatt tatcgccagc agctgattgg
    10381 taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgctgg attatcagga
    10441 gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc
    10501 cgccacgatt ctcggtgcag tcactcccgt tccggatacg ctttcagagt atgcgtttgc
    10561 cggattgcta cgtggcacca agaccgaagt ggtgaagtgt atctccaatg atcttgaagt
    10621 gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga
    10681 agggccgtat ggcgaccaca ccggttacta taatgaagtc gatagtttcc cggtatttac
    10741 cgtgacgcat attacccagc gtgaagatgc gatttaccat tccacctata ccgggcgtcc
    10801 gccggatgag cccgcggtgc tgggtgtcgc actgaacgaa gtgtttgtgc cgattctgca
    10861 aaaacagttc ccggaaattg tcgattttta cctgccgccg gaaggctgtt cttatcgtct
    10921 ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt
    10981 ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg atgatgacgt
    11041 caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg acccggcgcg
    11101 ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct cgcctgtctc
    11161 cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccggggg aaacccagcg
    11221 tgaatgggga cgtcccatca aaaaagatcc agatgttgtt gcgcatattg acgccatttg
    11281 ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta
    11341 tttatcgatc cgacagagaa agcgcatgac aaccttaagc tgtaaagtga cctcggtaga
    11401 agctatcacg gataccgtat atcgtgtccg catcgtgcca gacgcggcct tttcttttcg
    11461 tgctggtcag tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc
    11521 ttcgacgccg gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg aaatcaacct
    11581 ttacgcgaaa gcagtcatgg accgcatcct caaagatcat caaatcgtgg tcgatattcc
    11641 ccacggagaa gcgtggctgc gcgatgatga agagcgtccg atgattttga ttgcgggcgg
    11701 caccgggttc tcttatgccc gctcgatttt gctgacagcg ttggcgcgta acccaaaccg
    11761 tgatatcacc atttactggg gcgggcgtga agagcagcat ctgtatgatc tctgcgagct
    11821 tgaagcgctt tcgttaaagc accctggtct gcaagtggtg ccggtggttg aacaaccgga
    11881 agcgggctgg cgtgggcgta ctggcaccgt gttaacggcg gtattgcagg atcacggtac
    11941 gctggcagag catgatatct atattgccgg acgttttgag atggcgaaaa ttgcccgtga
    12001 cctgttttgc agcgagcgta atgcgcggga agatcgcctg tttggcgatg cgtttgcatt
    12061 tatctgagat ataaaaaaac ccgcccctga caggcgggaa gaacggcaac taaaacttta
    12121 ttcagtggca tttagatcta tgtcgcatcc ggcattcggc acacgatgcc tgatgtgacg
    12181 ctggcgcgtc ttatcaggcc tacattggtg ccggatcggt agaccggata aggcgttcac
    12241 gccgcatccg gcaagtggtt aaacccgctc aaacaccgtc gcaataccct gacccagacc
    12301 gatacacatc gtcgccagac caaactgaac gtctttgtgt tccatcagat tcagcagcgt
    12361 ggtgctgata cgcgcaccgg aacaacccag cggatgaccc agcgcgatcg caccaccgtt
    12421 gaggttgatc ttctcgtcaa tctgctcaat tagtcccaga tctttaatac atggcaggat
    12481 ctgcgcggca aatgcttcat tcatttcaaa cacgccgata tcactgacag aaagccccgc
    12541 ttttttcagc gccagcttcg aggccggaac cgggccgtaa cccataatcg atgggtcaca
    12601 accaacgacc gccatcgaac gcacacgagc gcgcggctta agacctaatt catgggcgcg
    12661 gctttcactc atcaccagca tggcagctgc gccatcggaa agtgcagaag atgtgcccgc
    12721 cgttaccgta ccgtttactg gatcaaacgc cggacgcagc gtggcgaggg cttccacggt
    12781 ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg
    12841 accaccagtt gggatgattt catttttaaa tgcagccgac tgcgtggcgg cccaggcgcg
    12901 ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt gcatacgcgc
    12961 cagcatttct gccgttaagc ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc
    13021 ggggtgaaaa tcgacgccgt gactcatcgg cacatggccc atatgctcca cgccgccaac
    13081 cagacatgcc tgcgcatcgc cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat
    13141 ggatgaacca cacaagcgat tgacggtaac cgccgggaca gagtgcggta cttctgccag
    13201 cagcgccgcg ttacgggcga tgttaaaacc ctgctccagc gtctgctgca cacaacccca
    13261 gtaaatatcg tcgagggccg ccgcttccag cgccgggtta cgcgccagca ggctacgcat
    13321 taaatgagcg gagagatctt ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc
    13381 catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc cttaagccgt
    13441 tttcaggtcg ccaaccggac gggctggctc aaccggggga tagtacggtt cgttatgacg
    13501 tgctttatta cgcagacctt ccggcacttc gtacagcggg ccgaggtgct gatattgctg
    13561 tgccatatcg aggtattttg cgctgccgag ggtgtccagc cagcggaacg cgccgccgtg
    13621 gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttctgccg gagtggcgat
    13681 aatgccttct tccagacagc gcaccacttc gttgaccatc gggatcatca tgcgggcgat
    13741 aatctcttct tcgctgaaat cgcgcttcgg ctggctgact tctgccagca ggtcgtcaac
    13801 agcggcgtct tcttctttct tcggcttacc tttgctgtct tctttataac gccagaaacc
    13861 gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg catcgcgata
    13921 atctttctgc atccgctgcg ggaagcctgc tgccatcacc gcctgggcgt gatgcgcggt
    13981 gtcaatgccc acgacatcca gcagatatgc cggacccatc ggccagccaa actgtttttc
    14041 catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcagctggc tgaaagcggc
    14101 gaaatacggg aacagcacgc ggttgacgaa gaagccgggg cagtcgttaa ccacaatcgg
    14161 cgttttgccc atcttgctcg cccaggcgac gactttcgcg atggtttcgt cagagctttt
    14221 ctcgccgcga ataatttcca ccaacggcat tcggtggacc gggttaaaga agtgcatccc
    14281 gcagaagttt tccggacgct ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt
    14341 gttagacgcc agcacggtat ccgggcgtac tttctgctcg gtttctgcca gcacggcttt
    14401 tttcactttc gggttttcaa caaccgcttc taccacaaca tccacgcggt caaatccggc
    14461 gtagtcgagc gttgggtgga tcgtggagat cacgccagcc agtttcagac catcgatctt
    14521 gccgcgctca agctgcttgt tcagtagttt cgcggcttca gtcatgccga gggctaacga
    14581 cttgtcgtta atatctttca taatcaccgg cacgcctttc cacgcagact ggtaagcgat
    14641 gccgccaccc ataatgcctg cgcccagcac agcggcctgt ttcggggttt caacgtcttt
    14701 ggtgagtttc ttcgctttgc cttttacata ttgatcgttg aggaaaatgc cgaccagtgc
    14761 gcgggcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt ttaaggcttc
    14821 ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg gggccggata
    14881 atgtttcccc gctgtttgtg cgaccatccc tttagcgatg gtgaagctca tggtggcttc
    14941 aatcttgctc agttttagcg gttccagctt cggctgacgt ttcgctttcc agtcgaggtc
    15001 gccgttaatg gcctggcgta aaatcgccat cgccccttca atcagttttt ctgctttgac
    15061 tacgccatcc accagaccga ttttcagcgc ctggtccgcg ccgacatctt taccggcggc
    15121 aatgatttcc agcgcgctgt ctgcaccgag catacgtggc atacgtacag aaccgccaaa
    15181 gccaggcatg atgcccagtt tggtttccgg caaaccgatg cgcagctccg gcgtcgccag
    15241 acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc cattgacggc
    15301 agcaatggtc ggcaccggca gatcttccag gcgattaaac acgctattgg caaaatgcag
    15361 ccactggctt agctgttctt cgggaacgag gaacagagac aaaaattcgg tgatatcggc
    15421 accgacgata aaggctgctt tgttcgaacg cagcagcaga ccttttaaat ctgattgctg
    15481 ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga gtttattaac
    15541 tgaacctggg gcatcaaata ccagttcggc aatgccgtct tccagccagt caaggtacag
    15601 ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca
    15661 gatgagtcga agtgtgtatt ttgtgttaaa aatatgcaaa caaaagatta aagaaatgcc
    15721 gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa gatgcggaga
    15781 cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcgct ctataaaaat
    15841 catatagcta ccttacaaga acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg
    15901 ttacttattc actccggcga gctgttcaat gtttttctcg acgatcatcc ctatccgttt
    15961 aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg
    16021 ctggtggatg gcgtgaacaa gccgaaactg tggttttatc tgccggttga ttactggcac
    16081 aacgtcgaac cgctgccgac ctccttctgg actgaagatg tggaagtgat cgcgctgccg
    16141 aaagccgatg gcattggtag cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt
    16201 ccggtgccgg agcgtgcgct gcaactgggt attgaggcca gcaacatcaa cccgaaaggg
    16261 gttatcgact acctgcatta ctatcgctcc ttcaaaaccg agtacgaact ggcctgtatg
    16321 cgtgaagcgc agaaaatggc ggtcaacggt catcgtgcgg cagaagaagc gttccgttct
    16381 ggcatgagcg agttcgatat caatattgcc tatctgactg cgaccggtca tcgtgatacc
    16441 gacgaacctt acagcaacat tgtggcgctc aacgaacacg ctgcggtgct gcattacacc
    16501 aaactggatc atcaggcgcc ggaagagatg cgcagcttcc tgctggatgc cggggccgaa
    16561 tataacggct atgccgctga cctgacccgt acctggccgg caaaaagtga caacgattac
    16621 gcacagctgg tgaaagacgt aaatgatgaa caactggcgc tgatcgcgac catgaaagct
    16681 ggcgttagct atgtggatta ccacatccag ttccatcagc gcatcgccaa actgctgcgt
    16741 aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga tcttaccggg
    16801 ccgtttatgc cgcatggtat cggccatccg ctgggcctgc aggtgcatga cgtcgccggt
    16861 tttatgcagg atgatagcgg tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc
    16921 tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat ctacttcatt
    16981 gaatcgctgc tggcaccgtg gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa
    17041 attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt gatccacgaa
    17101 aacaacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc tggttaattc
    17161 ctgcggcacc ggtcacggtc gttgaagaga tcaaaaaaag ccgcttcata acgatgttgg
    17221 cgcataccga tggcgttgag gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc
    17281 ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat tcacaacagc
    17341 tggggttctc tgacgatggg gagccggcgg gaacggcagg taaaccgatg ctcgcccaac
    17401 tgatgggcag cggcgtcggg gaaattaccg ctgtggtggt gcgctactac ggcggcatat
    17461 tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc
    17521 agctaacgac ccaacgcaag acgccattaa ccgaatatac tttgcaatgt gaatatagtc
    17581 agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc aacagtgatt
    17641 atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct gaattttccg
    17701 caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa gaataatccc
    17761 cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca ttacccgaat
    17821 cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc tggtagcact
    17881 catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg ccctcgccat
    17941 tggctctatg ctgtggtggc cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg
    18001 gtttctgata gtggtgctgt tctggaccgt gctgggtagt gtcggtgcgc tcccttttat
    18061 cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt tctctggcct
    18121 gaccaccacc ggggccacta cgctggtggg gctggattcg ctccctcacg ccatcctctt
    18181 ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttag cggttgcgat
    18241 actgcctatc ctcggcgtgg gtgggatgca gctctatcga gcagaaatgc ccggcccgct
    18301 gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta
    18361 tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gccggaatgg atgcctttga
    18421 tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac atgatgccag
    18481 tatcggttat ttcgatagcc cgactattaa cactatcatt gctatcttcc tgctgatctc
    18541 cggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga aggtttattg
    18601 gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt acgctggtgg ttatttgtac
    18661 cctcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta accaggcgtt
    18721 tttccaggtg gtgtcgatgg cgacaaccgc cgggtttacg actgacagca ttgcccgctg
    18781 gccgctcttt ttgccggtac tgcttttatg ttcagctttt atcggcggtt gtgccgggtc
    18841 aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg ggaaccgtga
    18901 actgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga atcgcgcact
    18961 gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat tggtgtttat
    19021 tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct ttgcctcggt
    19081 tgttgcgaca ttgaataacc tggggccagg gcttggcgtg gttgctgata actttaccag
    19141 tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt
    19201 ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga gtaatacgtg
    19261 aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc ctcctacctg
    19321 gcttcggaac tgaaagaact ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa
    19381 gaaccacagt gggaaaacta cgaccgtgtg gtcattggtg cttctattcg ctatggtcac
    19441 taccactctg cgttccagga atttgtcaaa aagcatgcga cgcggctgaa ttcgatgccg
    19501 agcgcctttt actccgtaaa cctggtggcg cgtaaaccgg agaagcgcac tccacagacc
    19561 aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc
    19621 attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat gatcaagctg
    19681 attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta taccgattgg
    19741 gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa
    19801 taagcataaa gaataaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    19861 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
    19921 ggtcagcggg gttctcctga gaactccggc agagaaagca aaaataaatg cttgactctg
    19981 tagcgggaaa gcgtattatg
//
LOCUS       DAZUGK010000079        19879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZUGK010000079 DAZUGK010000000
VERSION     DAZUGK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.079"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..697
                     /gene="lpxT"
                     /locus_tag="ACHY5W_002425"
     CDS             <1..697
                     /gene="lpxT"
                     /locus_tag="ACHY5W_002425"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFS4447312.1"
                     /translation="QIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQKLVESK
                     AFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTAVVLNQL
                     GQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAFMWRYFG
                     KVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDRLITFFD
                     KSLPGKNKHFQNK"
     gene            1109..1675
                     /gene="mepS"
                     /locus_tag="ACHY5W_002426"
     CDS             1109..1675
                     /gene="mepS"
                     /locus_tag="ACHY5W_002426"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFS4447313.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1856..3412
                     /locus_tag="ACHY5W_002427"
     CDS             1856..3412
                     /locus_tag="ACHY5W_002427"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS4447314.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMIAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3494..5308
                     /locus_tag="ACHY5W_002428"
     CDS             3494..5308
                     /locus_tag="ACHY5W_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4447315.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGNNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            5309..6403
                     /locus_tag="ACHY5W_002429"
     CDS             5309..6403
                     /locus_tag="ACHY5W_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFS4447316.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSHYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKILWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6403..7428
                     /locus_tag="ACHY5W_002430"
     CDS             6403..7428
                     /locus_tag="ACHY5W_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4447317.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7430..9019
                     /gene="yejF"
                     /locus_tag="ACHY5W_002431"
     CDS             7430..9019
                     /gene="yejF"
                     /locus_tag="ACHY5W_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFS4447318.1"
                     /translation="MTQTLLAIEDLSVGFRYQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(9023..9367)
                     /locus_tag="ACHY5W_002432"
     CDS             complement(9023..9367)
                     /locus_tag="ACHY5W_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFS4447319.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9700..10890)
                     /gene="bcr"
                     /locus_tag="ACHY5W_002433"
     CDS             complement(9700..10890)
                     /gene="bcr"
                     /locus_tag="ACHY5W_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFS4447320.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10918..11613)
                     /gene="rsuA"
                     /locus_tag="ACHY5W_002434"
     CDS             complement(10918..11613)
                     /gene="rsuA"
                     /locus_tag="ACHY5W_002434"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFS4447321.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11762..13522
                     /locus_tag="ACHY5W_002435"
     CDS             11762..13522
                     /locus_tag="ACHY5W_002435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4447322.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13647..13931
                     /gene="rplY"
                     /locus_tag="ACHY5W_002436"
     CDS             13647..13931
                     /gene="rplY"
                     /locus_tag="ACHY5W_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFS4447323.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(14070..15077)
                     /gene="yejK"
                     /locus_tag="ACHY5W_002437"
     CDS             complement(14070..15077)
                     /gene="yejK"
                     /locus_tag="ACHY5W_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFS4447324.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLTVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15259..15486
                     /locus_tag="ACHY5W_002438"
     CDS             15259..15486
                     /locus_tag="ACHY5W_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFS4447325.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15506..17266
                     /gene="yejM"
                     /locus_tag="ACHY5W_002439"
     CDS             15506..17266
                     /gene="yejM"
                     /locus_tag="ACHY5W_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFS4447326.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFAAYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQTFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17341..17417
                     /locus_tag="ACHY5W_002440"
     tRNA            17341..17417
                     /locus_tag="ACHY5W_002440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17375..17377,aa:Pro,seq:ggg)
     gene            complement(17519..>19879)
                     /gene="yejO"
                     /locus_tag="ACHY5W_002441"
     CDS             complement(17519..>19879)
                     /gene="yejO"
                     /locus_tag="ACHY5W_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFS4447327.1"
                     /translation="INGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQV
                     YGAANDPTIKGGRLIVEKDGIAVFAAIEKGGLLEVKEGGLAFAVDQKAGGAIKASTRV
                     MEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTAT
                     GVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTI
                     LDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAG
                     TMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENS
                     AWTIIADITTTNQNTLLNLANLAMSGANVIMMAEPVTRSSVTASAENFITLTTNTLSG
                     NGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFT
                     LGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDT
                     VRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESST
                     IRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGK
                     MSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRAD
                     VGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNNDVA
                     GTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         4850 a         5156 c         4812 g         5061 t
ORIGIN      
        1 gcaaatagtg ttgttgaata ttgtcggcct cgcgctgttt ctttcctggt atattcccgt
       61 taatcatgga ttctggttgc cgattgatgc ggatattttt tatttcttta atcagaaact
      121 ggtcgaaagt aaggcctttt tatggctggt tgcattgacc aacaatcgcg ctttcgacgg
      181 ttgttcactg ctggcgatgg gaatgttgat gctgagtttc tggctgaaag aaaacgctcc
      241 aggcagacga cgtatcgtga ttattggtct ggtcatgcta ttaactgcag tggtattaaa
      301 ccagctgggt caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga
      361 tattaaccgc gtcagcgaac tgctctctgt ccccacgaaa gatgcctcac gagatagctt
      421 tcccggcgat cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg
      481 caaagttgca ggtcttatcg cccttattat ttttgtggtt tttgcgtttc ccagagtaat
      541 gattggcgca cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg
      601 tttgccctgg gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc
      661 actaccggga aaaaacaaac atttccaaaa caaataactc acagtaatta acatcatcag
      721 gtttattttt atagtgagga taatcctgat gatgcgcacc gtgctttcat ctatcgaacg
      781 caaaaatcat tctctaagta aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa
      841 ttgaccaatc ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa
      901 ccgctcgcca agaccgcacc aatcgggtaa tctcgaactc gttttgcctc ggcggtagat
      961 tatcctcaca gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt
     1021 caacatgccc aggtaattag tctcgtgtcg cttggcattt ttttataacg atatttgtcg
     1081 ttaaggactt caagggaaaa caaacaacat ggtcaaatct caaccgattt tgagatatat
     1141 cttgcgcggg atccccgcga ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa
     1201 caccgcaaag aatatgcatc ctgagacacg tgcagtgggt agtgaaacat catcactgca
     1261 agcttctcag gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat
     1321 ggatcagtat gctgactgga aaggcgtacg ctatcgtctg ggcggcagca ctaaaaaagg
     1381 tatcgattgt tctggtttcg tacagcgtac attccgtgag caatttggct tagaacttcc
     1441 gcgttcaact tacgaacagc aggaaatggg taaatctgtt tctcgcagta atttgcgtac
     1501 gggtgattta gttctgttcc gtgccggttc aacaggacgc catgtcggta tttatatcgg
     1561 caacaatcag tttgtccatg cttccaccag cagtggtgtt attatttcca gcatgaatga
     1621 accgtactgg aagaagcgtt acaacgaagc acgccgggtt ctcagccgca gctaataaac
     1681 cgtttggatg caatcccttg gctatcctga cgagttaact gaaagcactg cttaggcagt
     1741 gcttttttgt tttcattcat cagagaaaat gatgtttccg cgtcttgatc caggctatag
     1801 tccggtcatt gttatctttt aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt
     1861 catacgcgct cccaattctg gacgtaagct cctgcttacc tgcattgttg caggcgtgat
     1921 gattgcgata ctggtgagct gccttcagtt tttggtggcc tggcataagc acgaagtcaa
     1981 atacgacaca ctgattaccg acgtacaaaa gtatctcgat acctattttg ccgacctgaa
     2041 atccactact gaccggcttc agccgctgac cttagatacc tgccagcaag ctaaccccga
     2101 actgaccgcc cgcgcggcgt ttagcatgaa tgtccgaacg tttgtgctgg tgaaagataa
     2161 aaaaacattc tgttcatctg cgaccggtga gatggacatt ccactcaatg aattgattcc
     2221 ggcgctcgac attaataaaa atgtcgatat ggcgatctta cccggcacgc cgatggtgcc
     2281 gaacaaaccc gcaatcgtca tctggtatcg caaccctttg ctgaaaaata gcggcgtctt
     2341 tgccgctctg aatctcaacc tgacgccttc actcttttat agttcacggc aggaagatta
     2401 cgatggcgtc gccctcatta ttggcaatac tgcgctatct accttttctt cacgtttgat
     2461 gaacgttaac gaattaaccg acatgccagt ccgtgaaact aaaattgcgg gcattcctct
     2521 gaccgttcgg ctttatgcag atgactggac atggaacgat gtgtggtacg catttttact
     2581 gggcggcatg agtggaactg tcgttggcct gctctgctat tacctgatga gcgtacgtat
     2641 gcgccccggc agagaaatca tgattgccat caagcgcgaa caattttacg tggcgtatca
     2701 accggtggtg gatacacaag ctttgcgagt aacgggcctg gaagtactgc tacgctggcg
     2761 gcatcctgtc gcgggagaaa ttcccccgga tgccttcatt aactttgccg aatcgcaaaa
     2821 gatgattgtg ccgctgactc agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt
     2881 agaaaaagtg ctgccggtag gcgtcaaatt tggtattaac attgcgccgg accatctgca
     2941 cagcgaaagc tttaaagcag atatccagaa actgctcact tccctgcccg cacaccattt
     3001 ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag ccacacaact
     3061 cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt gatgacttcg gcaccgggca
     3121 cagcgcgctt atctatcttg agcgttttac gctcgattat ctgaaaattg accgtggatt
     3181 tatcaacgcc atcggtacgg aaacgatcac ttcacccgta cttgacgcgg tgctgacgct
     3241 ggcgaaacgc ctcaatatgc tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg
     3301 atggctaagc gaacgcggcg ttaatttcat gcaaggctac tggattagtc gcccgttacc
     3361 gctggacgat tttgtccgct ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct
     3421 tacgtccctt attattcata gtgaaagcat gccggattgc ggctaatgat gagtaaaagg
     3481 aaatccgttg cagatgattg tgcgcatact gctgctgttt atcgctctgt tcacctttgg
     3541 tgcgcaggcg caggctatca aggaaagcta tgcctttgcc gtgctgggcg aaccccggta
     3601 cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat
     3661 aacgttgtca gccctcggca ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc
     3721 gggcgcacgc accgagcagt tgtacgacac gctatttacg acttccgatg acgaaccagg
     3781 cagttattac ccgctgattg ctgaaagtgc acgctatgct gacgattatt cctgggtgga
     3841 ggtcgctatt aatccgcgcg cccgttttca tgatggttcg cccattactg cccgcgatgt
     3901 agagtttact tttcaaaaat ttatgaccga aggcgtgccg caatttcgtc tggtctacaa
     3961 aggcaccacc gtcaaagcca ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg
     4021 caaagaagat atgttgagtc tgttttcgct gccggtattt ccagaaaagt actggaagga
     4081 tcacaaactt agcgatccgc tcgccacgcc tccgcttgcc agtggtccgt accgaattac
     4141 gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg aaagattact gggcagcaaa
     4201 cttaccggta aaccgtggac gctggaattt cgacaccatt cgctacgatt attacctcga
     4261 tgataatgtc gcctttgaag cgtttaaagc aggtgccttt gatttgcgta tggaaaacga
     4321 cgctaaaaac tgggccacgc gttataccgg taataatttc gataaaaaat acatcatcaa
     4381 agatgagcaa aagaacgaat cagcccagga tacgcgctgg ctggcgttta atatccaacg
     4441 tccggtattc agcgatcgcc gggtccgcga agcaatcacc ctcgcctttg actttgaatg
     4501 gatgaacaag gcgttgtttt acaatgcctg gagtcgcacg aacagttatt ttcagaatac
     4561 agaatacgcg gccagaaatt accccgacgc cgcggagctg gtgcttctgg caccaatgaa
     4621 aaaagatcta ccgccagaag tcttcacaca aatctaccag ccgccggtat ccaaaggcga
     4681 tggctacgat cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt
     4741 gctgaaaggt cagcaacgcg ttaatgccac tacgggtcag ccgctcagct ttgaattatt
     4801 gcttcctgca agcagcaata gtcagtgggt attgccgttc cagcacagcc tgcaacggct
     4861 gggtatcaac atggacattc gcaaggtgga taactcgcaa atcaccaacc gcatgcgcag
     4921 tcgcgactat gacatgatgc cgcgcctatg gcgggcgatg ccgtggccca gttccgattt
     4981 acagatttcc tggtcatcgg aatatatcaa ttccacttat aatgcccccg gcgtgcaaag
     5041 cccggttatc gactcgctga tcaaccaaat tattgccgcg cagggaaata aagaaaaatt
     5101 actgccgtta gggcgagcac tggatcgcgt attaacgtgg aattattaca tgctgccaat
     5161 gtggtacatg gcggaagacc gtctcgcctg gtgggataaa ttctcccaac ccgctgtgcg
     5221 ccctgtttac agtctgggta tcgatacctg gtggtatgat gttaataaag cggccaaact
     5281 gccgtcagcc agacaacagg gagagtagat gggcgcttat ctgattcgcc gtctgttgct
     5341 ggtgatccca acgttatggg cgattatcac tatcaacttt ttcatcgtgc aaattgcgcc
     5401 tggcggtccg gtcgaccagg ccatcgccgc cattgagttt ggtaatgccg gagtattacc
     5461 cggcgcaggc ggtgaaggcg ttcgcgccag ccatgcgcaa acgggtgtcg gcaatatcag
     5521 cgacagtcat taccgtggcg gacgcggatt agatccagaa gtgatcgctg agatcactca
     5581 tcgctacggt ttcgataagc cgctccacga acgttacttc aaaatactct gggactacat
     5641 ccgctttgat tttggcgata gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga
     5701 cagtctgccg gtttccatca ccctcggatt gtggagcacg ctgattatct atctggtgtc
     5761 gattccgtta ggcattcgca aagctgttta taatgggagc cgctttgacg tctggagtag
     5821 cgcatttatc atcatcggct acgccattcc ggcctttttg tttgccatcc tgctgattgt
     5881 cttcttcgcg ggcggcagct atttcgacct gttccctcta cgcggcctgg tttccgctaa
     5941 ctttgattcg ctgccgtggt atcagaaaat caccgattat ctgtggcata tcacgctgcc
     6001 ggtgctggcg acagtgattg gtggctttgc ggcgctgacc atgctgacca aaaactcatt
     6061 ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg taagtgagaa
     6121 aaacattctc tggaaacatg tgttccgcaa cgccatgctg ctggtgattg ccggttttcc
     6181 ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg attgaagtga tgttttcact
     6241 caatggcctt ggcttactgg gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt
     6301 tggtaccttg tatattttca ccctgattgg cctgctgctg aatattgtca gtgatatcag
     6361 ctatacgctg gtcgatccgc gtatagattt tgagggacgt taatgtcgcg actcagcccc
     6421 gtcaatcagg cccgttgggc gcgttttcgc cataaccgtc gcggctactg gtcgttatgg
     6481 attttcctcg ttttgtttgg tttgagtttg tgttctgaac ttatcgccaa cgataaaccg
     6541 ttgctggtgc gttatgacgg cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc
     6601 gattttggcg gcccgctggc aagtcaggct gattatcagg acccgtggct gaaacaacgg
     6661 ctggaaaata atggctgggt actgtgggca ccgattcgct ttggtgctac cagtatcaac
     6721 tttgctaccg ataagccctt cccttctccc ccctcccggc aaaactggct gggaacggat
     6781 gccaacggcg gcgatgtgct ggcacgtatt ctctatggca cgcggatctc ggttctgttt
     6841 ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc
     6901 tattacggcg gtaaagtcga tctctggggg caacgcttta ttgaagtatg gtcggggatg
     6961 ccgacgctgt ttttgattat tttactttcc agcgtcgtac agcctaactt ctggtggctg
     7021 ctggcaatta ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg ggcggagttt
     7081 ttacgtaccc gtaatttcga ctacattcgt gcggcgcagg cgcttggcgt cagcgatcgc
     7141 agtatcatcc tgcgtcatat gttgcctaat gccatggtcg cgaccctcac ctttttaccg
     7201 tttattttat gtagttcgat caccaccctg acctcactcg atttcctcgg cttcggtctg
     7261 ccgctcggtt caccgtcact cggtgaactg ctgttacaag ggaaaaataa ccttcaggct
     7321 ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt gctgatcttt
     7381 attggtgaag ccgtccgcga cgcatttgat cctaataagg cggtgtagca tgacgcaaac
     7441 tctgttagcg attgaagatt tgtcggtggg ttttcgctat cagcaaaccg tacgtacagt
     7501 agtcaatgat gtttcactac agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc
     7561 aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt ccccgccggt
     7621 tgaatatctc tccggcgata ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca
     7681 aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt tttcaagagc cgatggtgtc
     7741 gttaaatcca ttgcataccc tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg
     7801 gatgcgtcgg gaagcggctc gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg
     7861 ccaggcggca aaacggctga cagattatcc gcatcagctc tccggcggcg aacgccagcg
     7921 ggtgatgatt gcgatggcac tgttaacgcg accggaatta ttaattgccg atgaaccgac
     7981 caccgcactg gacgtctctg tccaggcgca gattttacag ctgttgcgcg aactgcaagg
     8041 cgagctgaat atgggcatgc tgtttattac tcataacctc agcattgtca gaaaactggc
     8101 ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct
     8161 atttgcctca cccactcatc cttacacaca aaagctactc aacagtgaac cgtcaggcga
     8221 cccggtgcca ttgccagaac ctgcctcaac gttgctggat gttgaacagc ttcaggttgc
     8281 cttccccatt cgcaaaggga ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa
     8341 catcagtttt acgctacgag cgggtgaaac actgggttta gtgggcgagt ccggttccgg
     8401 gaaaagcacg acgggactgg cgctgctgcg actgattaat tctcagggca gcatcatctt
     8461 tgacggtcag ccactgcaaa atttaaatcg ccgccagctg ttacctattc gtcatcgcat
     8521 tcaggtagta tttcaggatc caaactcctc gctcaaccca cgactcaacg ttttgcagat
     8581 tattgaggaa ggcttacggg ttcaccagcc gacgctttct gccgcacaac gcgaacaaca
     8641 agtgatagcc gtgatgcatg aagtgggatt agatcctgaa acacgccacc gttatccggc
     8701 ggagttctct ggtggtcagc gacaacgtat tgcgattgcc agggcattaa ttcttaagcc
     8761 ctcgctgatc atacttgatg aaccgacatc atcactcgac aaaacggtac aggcgcaaat
     8821 attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca
     8881 tgatttgcac gttgttcgcg cgttatgtca tcaggttatc gtactgcgac aaggggaagt
     8941 agtggaacaa ggaccgtgcg cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca
     9001 gctactggcg ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg
     9061 ccacaccaaa atttttcaat cggcatgttg cagcaaactc gtcctggcga tttacaaaca
     9121 ggcacggctc accttcacac tccagcaccg aacatggaac ttcgatgtcg cttaacgcct
     9181 ggcttagttt atacatcacc gaccatgcat tatcaccatc cgggctaagc aatttaagcg
     9241 ccaccaggct gttgtcaccg cacggtccat tttgcggaat cggttcaacc ttcgaacctg
     9301 cgaaaccagc agaccagcga tagttctgcg gcagtcgatg gacgattgag agttgtaatg
     9361 aagtcactta ttttccccgg aagcacattt acttcacaat ttgtttacat caattttaac
     9421 acatcatcaa caatccgtct ttatacagat cgtaaagatt gatgcctcac ctgcgtggcc
     9481 tctatggctc tgatttaagt aatatctgcg ggcgtggtca cgtttgcggg gctatatgta
     9541 cccgtttctg cgatctaact caaccttttt aactacaatg atgtgacttt ttacacaaat
     9601 tgattttaca taaaataaac atatatcggg ggaatgatag atttgtggtt gacatgcatc
     9661 aacaaaggaa gccttttagc ttcctcgttg tgcaatagat caccgttttt tcggccgact
     9721 ggcgtacaga cagaagagaa tggagctggt tgcgcagaat gcaattgacc aaatcatcgg
     9781 ccatgcagag ttaaaggtcg caagagaaag caatgcgcca acaattgctc ctatgccaaa
     9841 acggaaggtt cctgccagcg aagatgccgt tcccgccata tggggaaact catcaagaat
     9901 gaccgccatc gcgttggatg acaccatcga cacgcagccc acaaacgccg caacgccaac
     9961 caccagcgac caaaatccca gccccagcag cgcactgatg accatccacg ctgccataat
    10021 aaattgtatc cacaaccccg agcggaacat atttaacgcg ccaatacggc ggacgaagcg
    10081 gctattaaag atggtcatca cgaacaggaa aacaatgttt agtgcaaagt aataaccaaa
    10141 gttttccggc gcgacgtggt taatttcaat gtagacaaac ggtccggcgc ttaagaacga
    10201 gaacatcccg gcaaagctga aaccactggc cagcatataa ctcaggacac gcttatgacg
    10261 gaacagcgcc gcaaagttac caatagtggt acgaatgtga aaaggctggc gacgctccgg
    10321 tggtaaggtt tctttgatca gaaagaagat cattgccgaa gccagaatcg ccgccacggc
    10381 gaggatccag aagatgtaat gccagctcag ccacaccagc acccagccgc caactatcgg
    10441 tgccatcagc ggtgcaatgg ttgtcaccag catgacaaac gacatcatcc gcgagaactc
    10501 ttctttcggg tagatatcac gcatcaaagc gttgataacc acgctggccg cagccgccgc
    10561 cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc
    10621 caccgcggcg gcggcaaaca ccagcgtacc gccaagaacc accggcttac gcccgaagct
    10681 gtctgccatc ggcccgtaga ttaactgccc caacgcaaag cccagaatat aagtactgag
    10741 ggtcatctgc gtactgcccg ccggtacgcc aaactgcgct gaaattaccg gtagcgcggg
    10801 cagatacata tcaatcgaca gcggcatcaa catggccagc aggccaagga taaaaacaat
    10861 agcaaacgac gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta
    10921 gacgacgctg gcaatttctt cttcagttaa cggacgatat tcaccggggg ctaaatcagc
    10981 atccagcgta atgccgccaa tacgttcacg atgcagctca accacatggt tacccacagc
    11041 ggcgaacatg cgtttcacct gatgataacg cccttcgctg atggtcagac gaacctgcgt
    11101 tggggtaatc acttccagca ccgccggctt agtgagatct ttttcgttat gcagctgcac
    11161 gcctttagca aattgctctg ccgtatcgtc agctacgggt gattccagcg tcaccagata
    11221 ggtcttctcg caatgatggc gcggagaagt aatgcggtgt gaccactgac catcatcagt
    11281 cattagcacc agaccggtgg tatcgatatc cagccgccct gcagcgtgca gtttccacgc
    11341 cactggttca tcaagaaaat agagcaccgt tgggtgatca gggtcgtccg tggagcaaac
    11401 atagccctga ggcttattga gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt
    11461 gccatcgtaa gcgacatcat gttcaggaag cagtttgaac gctgcattac ggacgatttc
    11521 gccatcgacg gtgacacgat tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc
    11581 gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg attttgcctg taaaaacgga
    11641 ggtcgggcat tgacccgaaa atctgaactg ttgtctgccc agtatagcgg tctaataact
    11701 tccctcaagg gaaaaagaac cgtggcatac tattagcaga ataatctacc tacgcgagac
    11761 catgattttt acactccgcc catatcagca agaagccgtg gatgccacgc tcaaccattt
    11821 tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc ggtgcaggta aaagcctggt
    11881 gatagcggaa cttgcgcggc tggctcgtgg tcgcgtgctg gtgctggcac acgttaaaga
    11941 actggtggcg caaaaccatg caaagtatca ggcgctgggg ctggaagccg atatttttgc
    12001 cgccgggcta aagcgcaaag agagccacgg taaagtggta tttggcagcg tgcagtcggt
    12061 cgcccgtaat cttgatgcct ttcagggtga attttcgctg ttgattgtcg atgaatgtca
    12121 ccgtattggt gacgatgaag agagccagta tcagcaaatc ctcactcacc tgacaaaagt
    12181 gaatccccac ttacgcctgc tggggctgac tgccacgcct tttcgactgg gcaaaggctg
    12241 gatctaccag ttccattatc acggcatggt acgcggcgat gagaaagccc ttttccgtga
    12301 ctgcatttat gaactgccgc tgcgttatat gattaaacac ggctatctga cgccgccaga
    12361 acgactggat atgccagtag tgcaatacga tttcagccgc ttgcaggcac agagtaatgg
    12421 gctgttcagc gaagccgatc tcaaccgcga gctaaaaaaa caacaacgta ttaccccgca
    12481 catcatcagc cagattatgg agtttgctgc aacgcgcaaa ggggtgatga ttttcgccgc
    12541 caccgttgaa cacgcaaaag agattgtggg attactaccc gccgaagatg ctgcactgat
    12601 tactggcgac acccccggcg ctgagcgcga tgtgttaatt gaagatttta aagcccagcg
    12661 ttttcgctat ctggtcaacg tcgcggtact gaccaccgga tttgacgccc cgcacgtcga
    12721 tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt taccaacaaa ttgtcgggcg
    12781 cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt cttgattatg cgggtaatcc
    12841 tcacgatctc tacgcgccgg aagttggtac accgaaaggc aaaagtgaca acgttccggt
    12901 acaggttttc tgccctgcct gcggttttgc caacaccttt tgggggaaaa cgaccgccga
    12961 cgggacattg attgaacact ttggtcgccg ctgtcaggga tggtttgaag atgacgacgg
    13021 tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga
    13081 aaatgatatt gccgctcgcc gctgccgcga atgtgacacg gtactggttg atccggatga
    13141 tatgttaaaa gcggcgctac gactgaaaga tgcgctggta ttacgctgta gcggcatgtc
    13201 tttgcagcat gggcacgacg agaaaggcga atggttgaaa atcacctatt acgatgaaga
    13261 cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc gcccagcgta ccgccttcga
    13321 gcagcttttt atccgcccgc atacgcgcac accgggcatc cctctgcgct ggatcaccgc
    13381 cgccgatatc ctcgcccagc aagccttatt gcgacacccg gattttgtcg ttgcccgcat
    13441 gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg
    13501 ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg tataaggcga ttgagtatag
    13561 aatctcgccc gcttttgcat acgcaaagca gatcacttac ctgttgctgg gtcgcctgta
    13621 gcaggattaa tttaagagag aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc
    13681 agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa gttcccggca atcatctacg
    13741 gtggcaaaga agcaccgctg gctatcgagc tggatcacga caaagtcatg aacatgcaag
    13801 ctaaagctga attctacagc gaagttctga ccatcgttgt tgacggtaaa gaaatcaaag
    13861 ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa gctgcagcac atcgacttcg
    13921 ttcgcgctta attgctgaat aagttgtaaa aaaccccgct ccggcggggt tttttgtatc
    13981 tgcagattat gcctgattac ggtattgcta ttttttgcag gccagataag gcgttcacgc
    14041 cgcatctggc attcagaaca caaaaccgat taattgccgc cagacgtccg gcgctgcaat
    14101 tggtcgcgca aattcggcgg cgtgccttta atggtcaagg tatcggttgc tggatcccag
    14161 aagatgcgtt cacctaacag catcgcatca aagttaatag tcaagccacc gccgctaccg
    14221 gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg ggaagctctc ttccagttcg
    14281 tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc tgacgcctgc cagctctttc
    14341 gacagcgact ccagttcaat ctcttcgcct gcctgcagct gctcattaca gtagctataa
    14401 acctgctggc gcacgttctg tcgctctgct ttatccagtt gtgcttcggc agtgaaatca
    14461 tcaaccgctt gcaacagccc acggttttgc gctttcgcgt ttaatccttc gctggccccg
    14521 aggaaatcca taaagaaatc cgccactttg cgccctactc gccctttcag gaaagtgaga
    14581 tagcgcgtcg actctggatt ggtttcccat tcggttaaat cgatacgcgc aacaatatcc
    14641 gcatgattga tatcaagata atgggttggg ttgatgtcga gattttcgtt aacgcgcata
    14701 ctgctcaggt tgctcagaac tgccaccagc aaatactcca ccgtcagata acggtagtga
    14761 caaaacagca cgaatccacc gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcgg
    14821 ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc cctgacgctg taagcgcagc
    14881 gtctgcgcca gttcactctc ttcgctaaac aagccgtaag ctttattttt ggcgctatag
    14941 acccgatgca gttctgccac catctcaacg acggtttctg tcggttccag caatgaatcg
    15001 cgcaacacca gctcaaggtt ctgctcatca cgcttgataa gctggtgcag agcaatctgg
    15061 ttgatatcca gactcatgat aaactctcct ttaagaccgg gcggtattca accaccgccg
    15121 gttaagcgac gcaataaaag atggcggtca ttttacagaa ggcagcgtaa cgagaatgat
    15181 ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt aatatgttgc cctttcatca
    15241 actaactgat ttcgatttat gccacaaatt tcccgctaca gtgatgaaca ggttgaacaa
    15301 ttgctcgcag agctgctcaa cgtactggaa aaacataagg ctccgaccga cctttccctg
    15361 atggtgttag gcaacatggt cactaacctt atcaacacca gcattgcccc ggcccaacgc
    15421 caggcaattg ccaactcttt tgcccgcgcc ttacagtcct ctatcaacga agacaaagcg
    15481 cactaaggga aacagataac aggttatggt aactcatcgt cagcgctacc gtgaaaaagt
    15541 ctcccagatg gtcagttggg ggcactggtt tgcactgttc aatattctgc tttcgctcgt
    15601 cattggcagc cgttacctgt ttatcgccga ctggccgacc acgcttgctg gtcgcattta
    15661 ttcctacgta agcattatcg gccatttcag cttcctggtg ttcgccgcct acttgctgat
    15721 cctcttcccg ctgaccttta tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat
    15781 tctggcaacg gcgggaatga cgctattact gatcgatagc gaagtcttta ctcgtttcca
    15841 tctccatctt aatcccatcg tctggcaact ggttatcaac ccagacgaaa atgagatggc
    15901 gcgcgactgg cagctgatgt tcatcagcgt gccggttatt ttattgcttg aactggtgtt
    15961 tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt cgtcgacgct tcgcgcgccc
    16021 gctggccgca ttcttattta tcgcctttat cgcctcgcat gtggtgtata tctgggccga
    16081 tgcaaacttc tatcgcccga tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat
    16141 gacggcgcga cgttttcttg agaagcatgg cctgcttgat gcacaggagt atcaacgccg
    16201 tcttattgag caaggtaatc cagacgccgt ttccgttcag tatccgttaa gcgaactgcg
    16261 ctatcgcgat atgggcaccg ggcagaatgt cttgttgatt actgtcgatg gcctgaacta
    16321 ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt
    16381 cacgcgccat atgagctccg gcaacactac agacaacggc atctttggcc tgttctatgg
    16441 catctcaccg agctatatgg acggcattct gtcgacccgt acgcctgcgg cattaattac
    16501 tgcgcttaat cagcaaggct atcagctggg gttattctca tcagatggct ttaccagccc
    16561 gctgtatcgc caggcattgt tgtcagattt ctcgatgccg agcgtacgca cccaatccga
    16621 cgagcagacc gccacgcagt ggatcaactg gctgggccgc tacgcacaag aagataaccg
    16681 ctggttctcg tgggtctctt tcaatggcac taacattgac gacagcaatc agcagacatt
    16741 tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac cagatcaacc gcgtgctcaa
    16801 tgcactgcgt gattctggca aactggacaa cacggtggtg attatcactg ccggtcgggg
    16861 tattccactg agcgaagagg aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc
    16921 attagtgatt cactggccag gcacgccggc gcagcgtatt aatgcgctga ctgatcatac
    16981 cgatctgatg acgacgctga tgcaacgcct gctacatgtc agcacacctg ccagcgaata
    17041 ttcgcaaggt caggatttgt tcaaccctca acgccgtcat tactgggtta ccgcagcgga
    17101 taacgatacg ctggcaatta ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa
    17161 ataccgcact tacaacttac gtggtgaaag agtgaaagat gaaaaaccac agttaagttt
    17221 gttattgcaa gtactgacag acgagaagcg ttttatcgct aactgattaa ttataaatca
    17281 gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg tagtattaac agccacgagt
    17341 cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa
    17401 tcctctcgtg ccgaccaaaa atcccaaaaa aaaccaaccc ttacggttgg tttttttata
    17461 tctgcaatta attcgataaa cagaccgtga cacatcacag cctgtttatt ttctgttatc
    17521 agaacgtcca gaccacaccc gcctgagtat tccacggcga ttctacccct gcgccattgc
    17581 catagctgac tgacaaatga ccgcttaacg tcggggtaaa cgatgacctt atcccagcct
    17641 gataaacgcc acgggttcca gccacatcat tattaaattt gccatcgtca ttaactttca
    17701 cctggttaga atcggcgtat tcctgacgta cggcggcttt cagccagggt tccagcgtcg
    17761 taccgttttg caggtccatg tgatagctta ccgccgttcc cgcttcagcg cgtaatatcc
    17821 gggtatttcc cacatccgcg cgcatgccgt ttgataacgt gtagtcctga ccatctgtgg
    17881 taaagccggt aaaggccaga taaggtctaa cactccacaa tccgtcaacc caacggaacc
    17941 cgctctcgac atgagcaccc gcgccgttac tattgtaatc gccaaacgct gttgccccat
    18001 tactcatctt gccatggatg gtgttggcaa aacggtcaac tttcaccacc ccatcaacat
    18061 aggcaccgtt ctgatgctcc caaccggcat aagcacccag ggtatagcta tcgacattgc
    18121 ctttgccgcc gcgatcaaaa ccaatatcag aatgagagta accaaagaac aagccgcgaa
    18181 ttgtgctgct ttcttcacgg gagaaacggc tatcgatacc gagcgtcagg cccgtcaatg
    18241 tttgctcaaa accagctccc gcatcagtgg tcacgttgtt gcgggtgtta attgccgaac
    18301 tccacatcgc cgtatcgtaa ctaacgcctt ttacgctacc aagacgctca cgcacggtgt
    18361 ccagttctgc atcaaatacc agcggttgtg cggccgccat attcagcaca tcagtggttg
    18421 aaggggtaat ttgcgcgcga ttctctgcca gactccagct atggttacca ttatccagca
    18481 aggtatattc atacgtaccg atatcaacaa cgcctccggc attgcccaac gtaaatgcag
    18541 catcaccgcc gcccgttgtt accagtgtaa ggctatctcc tgctgccggg ctggcaccgg
    18601 tgtccgtcac gaatattttg aaatcacctg ttgcctgacc ggtgacgttg agctgatcgc
    18661 tctgatgatt agccatatcg gtacgcatat aaaaattgcc gtttcccgac agggtattgg
    18721 tggtcaacgt aatgaaattt tccgcacttg ccgtcactga tgaacgagtc actggctcag
    18781 ccatcataat cacatttgcg ccagacatcg caaggttggc taagttgagg agggtgtttt
    18841 ggttcgtcgt agtgatatcg gcaatgatgg tccatgcgct attttcgagc gaaacatccg
    18901 ctttgctggt atccacggca ccatgcgttc taaaagaagc gccctcagaa accactacct
    18961 tacccaccaa cattgcgggt gcataattta tttgcggctt catgacctca agaatgccat
    19021 cattccctct gactgaaacg ttaaccattg tgccagccca gacattagcg cgaccattgt
    19081 tgataaccat attttcagag atggctttag aggaatacgt tatacttcca ttctgatctg
    19141 atcgaccgag atcatatacc gcatttgcct gcactctcgt cccagtatcc tttcccagcg
    19201 attgcatagt ggcatgctca tcgaggatag tgtcaatggc ctgcgtgtcc tccataacaa
    19261 taagcccgga accatcatcc agttcataat tttttgacac accatctttt atactaaatt
    19321 ggcctttact gtttgtacca ctcacttcca gcgcattcgt tgagacaatt aattttccgc
    19381 ctgctttttt atcaacgcca gttgcagtcc cgccatccat aacctccagt aagccgccac
    19441 tatctacagt agtattataa gcgaagtcat tttcttcaac tcgcaaactt ccgccgtttt
    19501 ccaacagcat attgttagca ataccattct tgatttcgaa ctgaccgaga cggtttgttc
    19561 cgaatacctc catgacccgc gtgcttgctt taatagcacc gcctgctttc tgatctaccg
    19621 caaatgctaa tcccccctct ttaacctcca gtaatcctcc cttttcgata gcggcaaaga
    19681 cggcaatccc atctttttca acaattaagc gcccgccttt aatcgtggga tcattcgctg
    19741 cgccataaac ctgtagcaca ccattattga gcgtagtctg gtttgccgcg ccacccattt
    19801 gaacaatttg ataaccgtca tttaatactg agtattctgc ggtgccattc atttcaatgt
    19861 actgataccc gccgttaat
//
LOCUS       DAZUGK010000080        19791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZUGK010000080 DAZUGK010000000
VERSION     DAZUGK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.080"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHY5W_002442"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="ACHY5W_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4447328.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHY5W_002443"
     CDS             complement(1003..3117)
                     /gene="fusA"
                     /locus_tag="ACHY5W_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4447329.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHY5W_002444"
     CDS             complement(3214..3684)
                     /gene="rpsG"
                     /locus_tag="ACHY5W_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4447330.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHY5W_002445"
     CDS             complement(3781..4155)
                     /gene="rpsL"
                     /locus_tag="ACHY5W_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4447331.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHY5W_002446"
     CDS             complement(4281..4568)
                     /gene="tusB"
                     /locus_tag="ACHY5W_002446"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFS4447332.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHY5W_002447"
     CDS             complement(4576..4935)
                     /gene="tusC"
                     /locus_tag="ACHY5W_002447"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFS4447333.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHY5W_002448"
     CDS             complement(4935..5321)
                     /gene="tusD"
                     /locus_tag="ACHY5W_002448"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFS4447334.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5321..6043)
                     /gene="yheO"
                     /locus_tag="ACHY5W_002449"
     CDS             complement(5321..6043)
                     /gene="yheO"
                     /locus_tag="ACHY5W_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4447335.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHY5W_002450"
     CDS             complement(6210..7022)
                     /gene="fkpA"
                     /locus_tag="ACHY5W_002450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4447336.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            7243..7461
                     /gene="slyX"
                     /locus_tag="ACHY5W_002451"
     CDS             7243..7461
                     /gene="slyX"
                     /locus_tag="ACHY5W_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFS4447337.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHY5W_002452"
     CDS             complement(7510..8100)
                     /gene="slyD"
                     /locus_tag="ACHY5W_002452"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4447338.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(8195..8395)
                     /gene="yheV"
                     /locus_tag="ACHY5W_002453"
     CDS             complement(8195..8395)
                     /gene="yheV"
                     /locus_tag="ACHY5W_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFS4447339.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8405..10181)
                     /gene="kefB"
                     /locus_tag="ACHY5W_002454"
                     /pseudo
     CDS             complement(8405..10181)
                     /gene="kefB"
                     /locus_tag="ACHY5W_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(10181..10732)
                     /gene="kefG"
                     /locus_tag="ACHY5W_002455"
     CDS             complement(10181..10732)
                     /gene="kefG"
                     /locus_tag="ACHY5W_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFS4447340.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10863..12776
                     /gene="yheS"
                     /locus_tag="ACHY5W_002456"
     CDS             10863..12776
                     /gene="yheS"
                     /locus_tag="ACHY5W_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4447341.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12776..13798
                     /gene="yheT"
                     /locus_tag="ACHY5W_002457"
     CDS             12776..13798
                     /gene="yheT"
                     /locus_tag="ACHY5W_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4447342.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFILEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13792..14010
                     /gene="yheU"
                     /locus_tag="ACHY5W_002458"
     CDS             13792..14010
                     /gene="yheU"
                     /locus_tag="ACHY5W_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFS4447343.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            14064..14933
                     /gene="prkB"
                     /locus_tag="ACHY5W_002459"
     CDS             14064..14933
                     /gene="prkB"
                     /locus_tag="ACHY5W_002459"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFS4447344.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14988..15392)
                     /gene="yhfA"
                     /locus_tag="ACHY5W_002460"
     CDS             complement(14988..15392)
                     /gene="yhfA"
                     /locus_tag="ACHY5W_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFS4447345.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDVAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15694..16326
                     /gene="crp"
                     /locus_tag="ACHY5W_002461"
     CDS             15694..16326
                     /gene="crp"
                     /locus_tag="ACHY5W_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFS4447346.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16377..18467
                     /gene="yhfK"
                     /locus_tag="ACHY5W_002462"
     CDS             16377..18467
                     /gene="yhfK"
                     /locus_tag="ACHY5W_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFS4447347.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKQL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18534..19754)
                     /gene="argD"
                     /locus_tag="ACHY5W_002463"
                     /gene_synonym="dapC"
     CDS             complement(18534..19754)
                     /gene="argD"
                     /locus_tag="ACHY5W_002463"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFS4447348.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVMAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
BASE COUNT         4855 a         5403 c         4903 g         4630 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatataag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtcg tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gcgcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcatattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcacg agcagcaccg ccgtaggttt tagccagtac ggtggtgatt
      841 gcagcggtca gagtagtttt accgtggtca acgtggccga tagtaccaac gttaacgtgc
      901 ggttttgtac gttcaaattt ttctttagac acggctatat tccttactat agtgctctcc
      961 ccttcaggag agagcacggg actttggtat taacccttag gcttatttac cacgggcttc
     1021 aattacggcc tgagcaacgt tactcggcgc ttcatcatac ttcaggaatt ccatggtgta
     1081 tgatgcacga cctttggtca gagaacgcag ctgagttgcg tatccgaaca tttcagacag
     1141 cggtacttca gcgtggatct taacgccagt aacttcagat tcctgacctt tgagcatacc
     1201 acgacgacgg ctcaagtcac cgataacgtc accggtgttc tcttccggag tttctacttc
     1261 aaccttcatg atcggctcaa gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc
     1321 gatagaagca gccagtttaa acgccagttc agaggagtca acgtcatggt aagaaccgaa
     1381 gtgcagacga atacccatgt ctactaccgg gtagcctgcc agcggacctg ctttcagctg
     1441 ttcctggata cctttatcaa cggccgggat gtattcgcca gggattacac cacctttaat
     1501 gtcgttgatg aactcgtagc ctttcgggtt tgaacccggc tccagcgggt acatgtcgat
     1561 aacaacatga ccatactgac cacgaccacc agactgtttc gcgtgtttac cttcaacatc
     1621 ggtaactttc tggcggatag tttcacggta agcaacctgc ggtttaccta cgttcgcttc
     1681 aacgttgaat tcacgcttca tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc
     1741 cgcaatgatg gtctggttag attcttcgtc agtccataca cggaaagacg ggtcttcttt
     1801 agccagacgg cccagagcca gacccatttt ttcctggtca gctttggttt tcggttcaac
     1861 tgcgatggag attaccggct cagggaattc catacgttcc agaatgatcg gcgcatccgg
     1921 gtcacacagg gtgtcaccag tggttacgtc tttcagaccg atagcagcag cgatgtcgcc
     1981 cgcgcgaact tctttgatct cttcacgttt gttagcgtgc atctgaacga tacgaccgaa
     2041 acgctcacgt gcagctttca cggagttcag tacggtatca ccagagttaa ccacaccgga
     2101 gtaaacacgg aagaaggtca ggttaccaac aaacgggtcg gtagcgattt tgaacgccag
     2161 tgcagagaac ggctcgtcat cacttgcgtg acgttcagcc ggagtgtctt taccgtcgtc
     2221 caggataccg ttgatcgcag gtacgtcaac cggggatggc aggtaatcga ttaccgcatc
     2281 cagcatcgcc tgaacacctt tgttcttgaa cgcagaacca caggttacca ggatgatttc
     2341 gttgttcaga acgcgctgac gcagagcacc tttgatttct gcttcagtca gttcctcacc
     2401 acccaggtat ttttccatca gctcttcaga agcttcagct gcggattcga tcaggttctg
     2461 gtgccattcg ttagccagtt caaccatgtc tgccgggata tcttcgtatt cgaaggttac
     2521 gccctggtca gcgtcgttcc agttgatagc tttcattttc accaggtcaa caacaccggt
     2581 gaaatgttct tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt
     2641 tttgatctgg ttaacaactt tcaggaagtt cgcacccata cggtccattt tgttaacgaa
     2701 cgcaatgcgc ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg
     2761 aacaccacca actgcgcagt aaaccattac cgcaccatcg agcacacgca tggaacgttc
     2821 tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc
     2881 atactgctta gccataccag accagaatgc agtagtcgca gcggaagtga tggtaatacc
     2941 acgttcctgc tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat
     3001 tttatggttt acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc
     3061 gatgtgcgca ctgataccga tgttacggta gcgtgcgatg ggtgttgtac gagccatttg
     3121 tttcctcgtt tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga
     3181 agcgcccgcc tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt
     3241 gttggcttcg gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc
     3301 tgcagcatca gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg
     3361 agcagcttca acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac
     3421 tggtacctga taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac
     3481 gttttcgaga gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt
     3541 ctccagcgcg ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat
     3601 atttacaaat ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg
     3661 accaatgacg cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa
     3721 ctctacgagt ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca
     3781 ttaagcctta ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc
     3841 ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg
     3901 accgccacgg atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta
     3961 ggaagtcact tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt
     4021 cggtttttta ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc
     4081 ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg
     4141 gttaactgtt gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa
     4201 aacgtcctca cacaatatga ggacgccgaa ttttagggcg atgccgaaaa ggtgtcaaga
     4261 aatatacaac gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg
     4321 acgaaatcag tatagtcaat gggaatgatg tcgttcgaaa tttgaccagt caaaccgcgg
     4381 gcaataaggt cttcgttcag ggcatagacc ttaatggggg cattacgcag actttcaagg
     4441 tagcggttac cgtcaactgc ggcagttacg ccatcttgca ataacagcag ttcgtcgcct
     4501 tcactgagca gacgcagcag cgcagcaaaa tccgtcagcc agggtgaacg atgtaacgtg
     4561 tgcagcataa acgtctcaaa acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa
     4621 ggcatctgct tcgagcggcg tggcttcgac aacaaagggt gtctgcggat ctaacccgcg
     4681 ttcgcgcagt gaagccgcac aaacccagca ctgttcaatg tcgtacagac ccaacaattt
     4741 aaaagtggca atgtaatcac gcgccagcac tgcatcgggc ttttgtcctg gcagcagctg
     4801 aaaaacgcca tcagcaataa agaagacggc cagatcgtca gttaatgcgg aagttgccag
     4861 taaagcatct aaaccttccc ggcctgcagc tgtaccatga ggcgcagtag aaaaaacaaa
     4921 cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc
     4981 gccccaagtc cgcttaaggt aaatcccggc tgaaggtttg acgaggccaa tcccagcctt
     5041 ccggcctccg tttcatcaac aacgccacgg cgtaatgctg ccgctacgca gatattcagc
     5101 gccacaccat gttgcgcatt cagttgttgc caggcccgta cgaggtcaaa ttcgtcactt
     5161 gccggagagg tcaattggtt agcgttatag accccttccc gatagaaaaa gacgctgctt
     5221 aactcatggc catctgctat cagcgcctgc gcaaactgaa aagcactact cgcctgttgc
     5281 gtaccgtatg ctggcccggt caccacgatg gcaaaacgca ttacttatct tgcccctgga
     5341 aatcaccgct cttgaactgg cggatgtaga gatagacagt gtgtttggag atgttcaggc
     5401 ggtcagcaac ctggttgatc gcatctttaa tatcgaagat ccctttctcg tagagattca
     5461 gcacgatctg acgatttttg gcgttattag aaacattgcg atcggcattc acttcttcga
     5521 tggtgaactc cagcgtttgg gtaaccagat cttcaacaga agaggcaaag ttgacgcttg
     5581 aaccgacatc cggggtttct ggcggcacaa aagtgctcat aatctgcgag aagggaacat
     5641 caagattcat attgatgcac agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga
     5701 tagtcaggga cttcattaat acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc
     5761 tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc caggtcagta attggcgaac
     5821 caatcttccg gcctgtatgt tcaccgttag caatgcgaat ggctgaacat tttagatcct
     5881 gcaaagagtg caaaacgatt tcacagtggg agccaataag catcgctaac ccgtccacca
     5941 ccgcttcgta ggatttcaga atatcaaaat cggtctggtc gaaaggacgt tgatccagta
     6001 aatccaactc actggtttcg ttggttaaaa gcgacctgga catgaaaaaa aacactcctt
     6061 ttcaggagcc tgtcgttaac ttttcagggc aggctcatta atgatgcggg taactaaatt
     6121 aatacagcgg aggttccgct ttccagcact aattatatcc ggcctgtaat aaaaaaaccg
     6181 ccgcctggtc aggcggcggt tcttaatgct tattttttag cagaatctgc ggctttcgca
     6241 tcagcttccg gctttgcatc agccttcggc gctggtttca catccagcag ctctacgtca
     6301 aacaccaggg tagaattcgg tgggatcccc ggaacacccg ctttgccata agccagttct
     6361 ggtggaataa ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccaa
     6421 cccgggataa caccgtccag acggaaagaa agcggttcac cacgggtgta agagttgtcg
     6481 aactctttac cgtcgatcag cgtacctttg tagttcacta caacagtatc gctgtctttc
     6541 ggtgcttcgc ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc
     6601 acacctttct ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc
     6661 gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga
     6721 gtctgttcga tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca
     6781 gcgatcagct gatctttatc cagtttgatg cccagttttt cttgttcttt tagagagttt
     6841 tccatgtaac gacccagcga ggcacccagt gcataagctg atttctgatc gtcatttttg
     6901 aacgctgctt tgctgtcagc agttgtagca ggttttgctg cttcagcagc aaaagtgatt
     6961 ggtgcatgca gggcaacggc cattgtggtc gccagcagcg ttactttaaa cagtgatttc
     7021 atccatatct ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact
     7081 ataaagcgtt gtcgaacaaa tctacataca gacacgccct attatcatct attttcagac
     7141 tctttttgtt taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt
     7201 taagtagaat ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg
     7261 gaagcacgcc tggcagagct ggagagccga ctggcttttc aggaaatcac cattgaagaa
     7321 ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt
     7381 ctgctgaccg agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa
     7441 acgccaccgc cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcact
     7501 ttttcggtat tagtggcaac cgcaaccgcc gttaccttta ccgccacagc agccttcgcc
     7561 accgtgctca tgaccgtgat catggccatg accaccgcag caaccgtcgt ggtcgtgatc
     7621 gtggtgatga tcgtgcgcac cgtgaacgtg gccatgagcc agctcttctt cagtcgcttc
     7681 gcgaatcgcc acaacttcaa cgttgaattt caggttctga ccggccagca tgtggttacc
     7741 atcaaccacg acgtgatcgt cttcaaccgc agtgatttca accggtaccg gaccctggtc
     7801 ggtttcagcc aagaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt
     7861 aggaacacgt tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc
     7921 gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag
     7981 ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac
     8041 accgtcttct gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat
     8101 gatatctcct gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg
     8161 tactcactag cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca
     8221 cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc
     8281 cgcacttaac gcattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct
     8341 gcgcctgaca ggccgggcat tttgcgcccg caataaaacg ttttcggatt gccatcttta
     8401 ccctctactc aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct
     8461 ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcgg
     8521 catgcattgg gatgagctct cgcagcatcc gcatatccag gcggcgaaaa tgcagttgcg
     8581 cccgctgtgc ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta
     8641 acgcactgga gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg
     8701 cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata
     8761 tttccaccag cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg
     8821 cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc atcaccgtaa taaactttgt
     8881 agccatattt gcgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca
     8941 ttttatttgc catcagcaaa cgaccaatca cctgaccaaa acggccgaag cccacgacaa
     9001 tgacctgggg tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa
     9061 actggcggga tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa
     9121 gcgtcaccgt caccagcaac agcgccatct ggtcgccctg aaacaaccgt tgcgaagaag
     9181 cagtagaaaa gaggacaaag gcaaactcgc cgccctgact caacacgcca gcaaactgca
     9241 tccgctctga gctacgcacg ccatacagtc gcgccagcag atacagcacg agaattttca
     9301 ccgccaccaa cacaataacg cttaacacca cccacaacag atgggtataa agcacaccga
     9361 ggttgagtga catcccgaca gagataaaga acaaaccgag cagcaagcct ttaaagggat
     9421 cgatagccgt ttccagttca tggcgatatt cactttccgc cagcagcacg cctgcaataa
     9481 acgtaccgag cgccatcgac agccccaacg catccataaa caatgcggaa cccaatacca
     9541 gcagcagcgt cgcggcggtg aacacttccc gcacgccaga agctgcaata aaacggaata
     9601 ccggacgcag taaatagcgc ccgccaatca gcatgccgac aaacgccagc accttcatgc
     9661 cgatcttcat ccagtcgaaa tgttcgtctg ccgaacccgc caacaacggc actaacgcca
     9721 gtgccgggat caccgccaga tcctgaaaca gcagtaccga aaacccgagc tgcccggatt
     9781 cgctgcgatt catccctttc tcacgcatca attgcaacgc cattgcggtc gaagacatcg
     9841 caaggccaat gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc
     9901 ctgccagcaa cgccgcgctt aacagcacct gtgccgcacc tacgccaaaa atcgaacgcc
     9961 gcagttgcca aagcttggag ggattcaact caaggccgat gataaacatc aggaacacca
    10021 caccgagttc cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agtccccatg
    10081 ggccaattgc aatccctgcc agcaaatatc ccaacacagc gccaataccc agccgcgaag
    10141 agaaacagca ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca
    10201 gcggatttgc cagccagtca ccgtaggctc tggcgtggct cgccagctcc tgtgcgcttt
    10261 gccgtctcgc ccagtaaata atgatgggac ttaaccaatg catccggcac atgcccgccg
    10321 ccagttcaaa ggggcgcagg acatcgctca tcgggtagcg attcaacgcg tcataacggt
    10381 aagcactttc cggctcgccg gtggtgatca cgctacgcca gtactttccc gccagttggt
    10441 ttcctcccgg cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta
    10501 gcgccgggca actataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca
    10561 gtaatgcctg ctcacggggg atatcaataa aaaaatcggg atagtgcgca taaaggtcgt
    10621 gcacggtaac attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt
    10681 cctgagattc cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc
    10741 tcccggtatg gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca
    10801 gctcgcccat acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc
    10861 ttatgattgt tttctcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg
    10921 ccaccgccac catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta
    10981 aatctaccct gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct
    11041 ttccgggaag ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg
    11101 cgctggaata tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctgcacg
    11161 acgccaacga acgtaacgac gggcacgcca ttgcgaccat tcatggcaag ctggatgcta
    11221 ttgacgcatg gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca
    11281 atgaacaact ggagcgcccg gtaagtgatt tctctggtgg ctggcgtatg cgtcttaacc
    11341 ttgcccaggc gctgatttgc cgttcagact tgctgctgct cgacgaaccg actaaccacc
    11401 tcgatctcga tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga
    11461 tcctgatctc tcacgaccgc gacttcctcg atccgatcgt cgataaaatt attcatatcg
    11521 aacaacaaag catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca
    11581 cccgtctggc gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcacatctgc
    11641 aaagttatat cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca
    11701 ttaagatgct cgagcgtatg gagctgattg ccccggcgca cgtcgacaat ccgttccgct
    11761 ttagcttccg cgcgccggaa agtctgccaa atccgttact gaagatggaa aaggtcagcg
    11821 caggctatgg cgatcgcatt attctcgact cgattaaact gaatctggtc cccggttcgc
    11881 gcattggtct gttaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg
    11941 gtgaacttgc gccagtcagt ggtgaaatag gcctggcaaa agggatcaag ctcggctact
    12001 tcgcccagca tcaacttgaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac
    12061 gtttagcgcc gcaggagctg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc
    12121 agggcgataa agtaaccgaa gaaacgcgcc gcttctcagg tggggaaaaa gcccgcctgg
    12181 tgctggcatt aattgtctgg cagcggccga atctgctgct gctcgacgaa ccgactaacc
    12241 accttgacct tgatatgcgt caggcactca ccgaagcatt aatcgagttt gaaggcgcgc
    12301 tggttgtcgt ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg
    12361 ttcacgatcg taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga
    12421 gcgacgtaca aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcgaacagcg
    12481 cccaggcacg taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccagccac
    12541 tgcgtaaaga gattgcccgt ctggaaaaag agatggagaa gctgaacgcg caactggcgc
    12601 aggcggaaga gaaactcggc gatagcgaac tgtatgacca gagccgtaaa gcggagttga
    12661 ccgcctgcct gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcat
    12721 ggctggaagc ccaggagcag cttgagcaga tgttgctgga aggccaaagc aactgatggc
    12781 gcagataacg acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg
    12841 cggctttagc aattgtcatc tgcaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa
    12901 attcaccccg cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg
    12961 gagtgaagcc cctgcacagg cgcggcataa accgcgttta gtggtatttc acgggctgga
    13021 aggcagtctc aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg
    13081 gctgggcgtg gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat
    13141 ttaccattcg ggcgaaaccg aagacgccag ttggttttta cgctggctgc aacgcgaatt
    13201 tggtcatgcg ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt
    13261 gctggcaaaa gaaggcaatg atctcccggt tgatgcggcg gtgattgtct ctgcgccgtt
    13321 tattctggag gcctgtagtt accatatgga aaagggcttt tcccgcattt atcagcgtta
    13381 cttgctgaac ctgttaaaag ccaatgccgc acgcaagctg gctgcctacc ccggaacgct
    13441 gccgataaat ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat
    13501 caccgccagg attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat
    13561 gccgatgctg aaccggatcg ccaaaccgac gctgattatt cacgctaaag acgatccgtt
    13621 tatggatcat caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact
    13681 gactgaacat ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg
    13741 gctggagtca cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt
    13801 ccgtggcaag acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt
    13861 gaaggcaccg attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa
    13921 cgccagctac agtgcggaga agcggtgctg gtatggtcag agctgcacga aacggtcaat
    13981 atcatgccgc gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat
    14041 gatagctaag tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat
    14101 ccagcggcgc ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa
    14161 atctgcatgc agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg
    14221 acatggcgat ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc ggccccgaag
    14281 cgaacgactt tggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga
    14341 aatctcgcaa atatctgcat acctacgacg aagccgtacc ctggaatcag gtaccgggaa
    14401 cattcacccc ctggcaacct ttaccagaac ccactgatgt actgttttat gaaggtttac
    14461 acggcggcgt agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg
    14521 tggtgcctat cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg
    14581 ggcactcacg agaagcagtg atggactcag tagtgcgttc aatggaagac tatatcaact
    14641 acatcacacc gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca
    14701 cttcaaaccc gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc
    14761 attttcgtaa tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat
    14821 tcatttccca catcaatacg ttagtggtgc ccggcggcaa aatgggtctg gcaatggaat
    14881 taattatgct gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactctgct
    14941 attacgccgg ataaaatact atccggcttc acaacgggat agttaaatca cgcggcaacc
    15001 acttcatacg agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat
    15061 ttctcagcag agagatcaac cgcacgcgca accgctacgt ctttcaggtc gcgaccggtg
    15121 acgataaaat gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag
    15181 gtcaatttta cttcacaatc gaccacatcc tgacgccctt tttgcaggat cgaaaccaca
    15241 tcgatggcac tgcaaccacc cgccgccatc aacaccattt ccatcggact tggtgcttta
    15301 tcgcctgagt tgccgtccat taaaatctgg tgaccagagg cagattcgcc cagaaaagtt
    15361 aacccttcga cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc
    15421 tgacagagta cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag
    15481 cgagacacca ggagacacaa agcgaaagct atgctaaaac agtcaggatg ctacagtaat
    15541 tcattgatgt actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc
    15601 cccttcccag gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct
    15661 ctggagaaag cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac
    15721 ccgactctcg aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg
    15781 cttattcacc agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca
    15841 gtgctgatca aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat
    15901 tttattggcg aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg
    15961 aaaaccgcct gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta
    16021 aacccggaca ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca agtcacttca
    16081 gagaaagtgg gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctc
    16141 aatctggcaa aacaaccaga cgctatgact cacccggacg gtatgcaaat caaaattacc
    16201 cgtcaggaaa tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag
    16261 atgcttgaag atcagaacct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact
    16321 cgttaatccc gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt
    16381 ggcgcagact gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat
    16441 gtttgcccgt ggccgttggg ttaatgcttg gcgaattacg attcggtctg ctcttctctc
    16501 tcgttcctgc ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac
    16561 gcttaatcat tggtgcgtcg ctgtttgcca cctgtagctt actgacacag ctgctactgg
    16621 caaaagatgt tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg
    16681 ctgagttggg gccattgcac gcaaaattgc ttcccgcatc gctgctggca gccattttta
    16741 ccctcagtct ggcgggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca
    16801 ctctctggta cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc
    16861 gcgagtcact aagtctgctg taccgtgaac tggcagatta ttgtgaagcc aaatacagcc
    16921 tgcttaccca gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg cgccagcaaa
    16981 aagcggtcga tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata
    17041 atactgacta caagcggatg ctgcgtattt tccaggaggc gctggattta caggaacata
    17101 tttcggtcag tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt agccatgcgg
    17161 aagaagttat ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg
    17221 acattcttta ccatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg
    17281 aaaaaatcgc ccgccagcat ccggataatc cggttgggca attctgctat tggcacttca
    17341 gccgcatcgc ccgcgtgctg cgcacccaaa aacagctcta tgcgcgtgac ttactggccg
    17401 ataaacagcg gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc
    17461 cggcgctacg caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca
    17521 ccgcgctgca tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa
    17581 atggctatgg tgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt
    17641 taatcattgc gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgaccttga
    17701 tgctgattac cacccttgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg
    17761 tcggttttac tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt
    17821 acatccttcc gcgtcttatc gataccatta ttggttgttt aattgctttc ggtggtaccg
    17881 tctggctgtg gccgcagtgg cagagcgggt tattgcgtaa aaacgcccat gacgctttag
    17941 aagcctatca ggaagcgatt cgcttgattc tgagcgagga tccgcaacct acgccactgg
    18001 cctggcagcg aatgcgggta aatcaggcac ataacactct gtataactca ttgaatcagg
    18061 cgatgcagga accagcgttc aacagccatt atctggcaga tatgaaactg tgggtaacgc
    18121 acagtcagtt tattgttgaa catattaatg ccatgaccac gctggcgcgg gaacaccggg
    18181 cattgccacc tgaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt
    18241 gtcagcagcg actggagtat gacgaaccgg gtagttctgg cgatgccaat atcatggatg
    18301 cgccggagat gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacagcggg
    18361 ttattggtca tctgaacacc atgcacacca tttcgtcgat ggcatggcgt cagcgaccgc
    18421 atcacgggat ttggctgagt cgcaagttgc gggattcgaa ggcgtaatgc aggctaaacc
    18481 atcggataag gcattcacgc cgcatccgac atcttttgcc tgatgcgtct tatttacgcc
    18541 ccaaccacct tcgccaccgc gtgggcgaaa cgttgcatcc cttcatcgat atccgcatct
    18601 tccaccacca gcgacggtgc aaaacgcatc acatccggtc cggcattcag caccattacg
    18661 ccagcctctg cgcccgcata caggaaatca cgcgcctgac ctttgtactg tggtttcagc
    18721 tccgcgccaa ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg
    18781 atcttctgca aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca
    18841 ggggtattga tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg
    18901 taggtggaac cgtgagaacc aggatgaaac gcagaagcaa tttccgccgt ggtcagcatg
    18961 gcgctaatcg ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcacg
    19021 ccgtagtgca tgtaagcaaa caaatcaccg gtccgcccca tcccgcactg cacttcatca
    19081 aacaccaata atgcctgata ttgatcgcac aactcacgca agccctgcaa aaactctggc
    19141 gtcgctgcca tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtgtga
    19201 tcatccatca ccgctttcac tgcgtggaga tcgttaaagg gaacgtggat gatgtctgct
    19261 ggttttggcc caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc
    19321 gagcgaccat gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc
    19381 acacaggcgt aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccagagttc
    19441 ataaacacca cacgttcggc aaacgttgcc tcaatcagtt tacgcccaag acgcagcgcc
    19501 ggttcattgg tgaaaacgtt actgatatgc cacagagttt cgccctgggt ttttaacgcg
    19561 ttcaccagcg caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg
    19621 acatactcct tgccttgctg atcccagatt cggctgccct gaccttttac cggaataaac
    19681 tctgccggag cataaatcgg caggatcact tcatcgaaag tcgcgcgtgt aattgctgtt
    19741 tgttcaattg ccatctcatg atcaccctgt tacgcataaa taaatgtgaa a
//
LOCUS       DAZUGK010000081        19601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZUGK010000081 DAZUGK010000000
VERSION     DAZUGK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.081"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHY5W_002464"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHY5W_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFS4447349.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2761)
                     /gene="hemX"
                     /locus_tag="ACHY5W_002465"
     CDS             complement(1574..2761)
                     /gene="hemX"
                     /locus_tag="ACHY5W_002465"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFS4447350.1"
                     /translation="MTEQEKTSAVVEDTREAVDTTPQPVVAEKKSKNSTALILSAVAI
                     AIALAAGVGLYGWGKQQAVSQTATSDALASQLTALQKAQESQKAELEGIIKQQAAQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTEDATTKAFLDEVDQL
                     SQQSISMDLPETLQSQTMLEKLMQTRVRNLLAQPAAGATEAKPAPAPAPQADTPAAAP
                     QGE"
     gene            complement(2783..3523)
                     /gene="hemD"
                     /locus_tag="ACHY5W_002466"
     CDS             complement(2783..3523)
                     /gene="hemD"
                     /locus_tag="ACHY5W_002466"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFS4447351.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DLLSALGEGDLLFALSQHAVAYAQSQLHQQALKWPLLAGYFAIGRTTALALHTVSGHQ
                     VRYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGETLTARGAEVAFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLHQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWNDIKVADNADNDALLRALQ"
     gene            complement(3520..4461)
                     /gene="hemC"
                     /locus_tag="ACHY5W_002467"
     CDS             complement(3520..4461)
                     /gene="hemC"
                     /locus_tag="ACHY5W_002467"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFS4447352.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLKNRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4848..7394
                     /gene="cyaA"
                     /locus_tag="ACHY5W_002468"
     CDS             4848..7394
                     /gene="cyaA"
                     /locus_tag="ACHY5W_002468"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFS4447353.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKIDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7434..7754)
                     /gene="cyaY"
                     /locus_tag="ACHY5W_002469"
     CDS             complement(7434..7754)
                     /gene="cyaY"
                     /locus_tag="ACHY5W_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFS4447354.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7802..9127)
                     /locus_tag="ACHY5W_002470"
     CDS             complement(7802..9127)
                     /locus_tag="ACHY5W_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS4447355.1"
                     /translation="MKGQIITVALILLGHTFLTPAAQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSNNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NAVHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDHSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVRELHNRDIIIPLTDESCRNAAAEVQTLKP"
     gene            complement(9124..10602)
                     /locus_tag="ACHY5W_002471"
     CDS             complement(9124..10602)
                     /locus_tag="ACHY5W_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS4447356.1"
                     /translation="MHYRNVVCLLACSLVLSSAWGCRLSAPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPQTDLTTDYLYEGAQAWYPHYS
                     WYSDGRNILYAGEVVQNPPGTPPVDVASFKAWGDFAADKHSLYFEGKRTDDNGGRNSL
                     DIKTLHQVEFRPPWAPDLLGLILRDANFLYINGHRLADPESFRVLAQKSWDQRGKFST
                     TFNPCVAVPFGPWDTLARTRTKILINGEQLVADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLLLLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10791..10994
                     /gene="yifL"
                     /locus_tag="ACHY5W_002472"
     CDS             10791..10994
                     /gene="yifL"
                     /locus_tag="ACHY5W_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4447357.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11031..11855
                     /gene="dapF"
                     /locus_tag="ACHY5W_002473"
     CDS             11031..11855
                     /gene="dapF"
                     /locus_tag="ACHY5W_002473"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4447358.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11852..12559
                     /gene="yigA"
                     /locus_tag="ACHY5W_002474"
     CDS             11852..12559
                     /gene="yigA"
                     /locus_tag="ACHY5W_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFS4447359.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12556..13452
                     /gene="xerC"
                     /locus_tag="ACHY5W_002475"
     CDS             12556..13452
                     /gene="xerC"
                     /locus_tag="ACHY5W_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4447360.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13452..14168
                     /gene="yigB"
                     /locus_tag="ACHY5W_002476"
     CDS             13452..14168
                     /gene="yigB"
                     /locus_tag="ACHY5W_002476"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFS4447361.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14252..16414
                     /gene="uvrD"
                     /locus_tag="ACHY5W_002477"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14252..16414
                     /gene="uvrD"
                     /locus_tag="ACHY5W_002477"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFS4447362.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16459..17361)
                     /locus_tag="ACHY5W_002478"
     CDS             complement(16459..17361)
                     /locus_tag="ACHY5W_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFS4447363.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(17444..18208)
                     /gene="yigE"
                     /locus_tag="ACHY5W_002479"
     CDS             complement(17444..18208)
                     /gene="yigE"
                     /locus_tag="ACHY5W_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFS4447364.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18370..18426
                     /gene="ysgD"
                     /locus_tag="ACHY5W_002480"
     CDS             18370..18426
                     /gene="ysgD"
                     /locus_tag="ACHY5W_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFS4447365.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18578..19528
                     /gene="corA"
                     /locus_tag="ACHY5W_002481"
     CDS             18578..19528
                     /gene="corA"
                     /locus_tag="ACHY5W_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFS4447366.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4525 a         5096 c         5159 g         4821 t
ORIGIN      
        1 agctcagggt aaatattcac tcagaaagtt attactcagg aagccaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg tgtcaggaag gccccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tttgctggcg cagcactttt tccagctgtt ccggattgtt tgttttcagt
      661 cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa
      961 ctccatgctc ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt
     1321 gcacggcgac gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga
     1501 tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag
     1621 gtgcaggtgc aggtttggct tccgttgccc ccgctgccgg ttgtgccagc aggttacgca
     1681 cgcgagtctg catcagtttt tctagcatcg tctggctttg cagtgtttcc gggagatcca
     1741 tcgaaatact ttgctgactt aactggtcca cctcgtcgag gaatgctttg gtcgttgcat
     1801 cttcagtatc gtaataagca cgaacccagg tggagacatt ctccagtgcc tggcggtaag
     1861 tctcttcctg atgacgtggc acagcttgtg cggcgaccag cagacgggaa cgaatatttt
     1921 cgcgcagata gatatcctga tttggcgcta acagcggtac ggcggtgtca tcacgacggc
     1981 gaatcgtaat gaagttgtcc ataaagttct gccagctttt ttgcagattg atacgccatt
     2041 cgcttatgga actggaaagc tcttcaccgt cggaatccat cggcgaacca tcgctgtcat
     2101 tatcggccag acgcaggtta tctacctgat ttgaaagctg attaagctta aggatgatgc
     2161 cgtcataatc cacctgcgat actgcagaaa ggctggcgat atcatcggta attgcccgac
     2221 gaacggtaat cagacttggg tcattcatat ccgccaggct ggcgtctgca cttttcagca
     2281 acgctgcagc ggtcgtgacg tcctgatcgc tccacagctt ccgtccggcg agtttcacca
     2341 gaaaatcggc ctgagccagc agccaggttt tagcatcgct gccggaaatg gtggcgacct
     2401 tttgttggac ttcatccaac tgttttgcca gcgtttcttg ctgacgattc gcctgttcaa
     2461 gctgtgcagc ttgttgcttg ataatgcctt ccagctcggc tttttggctc tcctgggctt
     2521 tttgcagcgc cgtgagttgg cttgccaggg catcgctggt ggcagtctga ctgacggcct
     2581 gttgtttacc ccagccatac aaaccgacgc ctgctgccag agcaatggcg atagccaccg
     2641 cactgagaat cagagcggta ctgttcttac tcttcttttc tgcaacgaca ggttgtggcg
     2701 tggtgtccac ggcctccctg gtatcttcaa ccacggcgga ggttttttct tgttccgtca
     2761 ttatggcttc ctgttatgag agttattgta atgcccgtaa tagcgcatcg ttgtcagcgt
     2821 tatcggcgac cttaatgtca ttccagccca gttcccgggc gagtttcgcc aaacgctcac
     2881 tgacgaccaa aagccgacag cgtagtaacc agtgctcacg ataccattgt gggatcaatg
     2941 accagagttg atgcaacatt tcaccgctgg taacaacgac ggtagtcacc tcgcgagatt
     3001 gccaacgcat cgcctcttct gcaccgtcat aatgaatggc gcaccgttga taacattcac
     3061 aaaaagcgac ttcagcaccg cgtgctgtca gagtttctcc aatcagctca cgaccgccgt
     3121 tgccacgtaa tatcagcgca cgtttgccag caatattttg taattcaggt aattgtagca
     3181 agacttcact aatttcccga tcctgcggat aacgaacctg gtgtccactc acggtatgca
     3241 gcgccagcgc agtggtgcgg ccgatagcga aataaccagc aagtaatggc cacttaagag
     3301 cttgctgatg cagttgcgat tgggcataag caacggcgtg ttgcgaaagg gcaaacaaca
     3361 gatcgccttc accaagtgct gatagcaggt cggcaagttg tggtaattgc cgacctgggg
     3421 aaaactctat cagtggaaaa tgccaggcca cctgccccag tgtgcgcaga cggctcacta
     3481 actcttctcc agcgggagac gggcgggtga ccaggatact catgccgggg cgtctccgtt
     3541 atagacttca gcgaggatct cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat
     3601 gcccatttgt tcggcatcct gcggcgcacc gcggcgttca ccgcgaataa tttgcgaacc
     3661 gtccggcgcg ccgaccagcg cacgcagcca gatttcgcca tcaataagct cggcgtagct
     3721 accaattggc acctgacatc cgccttcgag acgggtattc atggcgcgtt ctgcggtaac
     3781 gcgcagtgca gtttcgtggt gattcagcgc ggcaagcagc tcgcgagtgc gtgtatcatc
     3841 aaggcggcat tcaataccga ctgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg
     3901 caacgcggcg cgaatgcggg actccagacc taaacgtttt agtccggcta cggcaagaat
     3961 gatggcatcg tattcgccgt tatccagttt gctcaggcga gtgccgacgt tgccgcgcag
     4021 ggagcggata atcagatccg ggcggcgttc agccagttgg cactggcgac gtaaactgga
     4081 cgtcccgacg atactgcctg ccggtaacgc atccagactg tcatagttat tggacacaaa
     4141 ggcatcgcga ggatcttcac gctcgcaaat agtgaccagc cccagacctt gcgggaattc
     4201 aaccggcaca tctttcattg aatgtacggc gatatcggcg cgatttttga ggagcgcgac
     4261 ttccagctct tttacaaata agccttttcc gccgactttc gccagcggcg tatcaagaat
     4321 cacatcgccg cgcgtcacca tcggtaccag ttcaacgacc aggcccggat ggctcgccat
     4381 caacttgtct ttgacatagt gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat
     4441 tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc atccgtggtc catcctaaca
     4501 tccttgccag agtgatgtca gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa
     4561 tgaggtctgg cagtggatcc tgacaggcgt ttcacgccgt cgtaataagg aatttacaga
     4621 gaataaacgg tgctacactt gtatgtagcg catctttctt tacggtcaat cagcaaggtg
     4681 ttaaattgat cacgttttag accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa
     4741 aagtggtaac ggttaccttt gacatacgaa atatcccgaa tgccgcgtgt taccgttgat
     4801 gttggcggaa tcacagtcat gacgggtagc aaatcaggcg atacgtcttg tacctctata
     4861 ttgagactct gaaacagaga ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg
     4921 ctgctatggg gcctgcattc caacaggtct acagtctact gccgacattg ttgcactatc
     4981 accatccgct aatgccgggt taccttgatg gtaacgttcc caaaggcatt tgcctttaca
     5041 cgcctgatga aactcaacgc cactacctga acgagcttga actgtatcgt ggaatgtcag
     5101 tacaggaccc gccgaaaggt gagcttccaa ttactggtgt atacaccatg ggcagcacct
     5161 cgtccgtagg gcaaagttgt tcctctgacc tggatatctg ggtctgtcat caatcctggc
     5221 tcgatagcga agagcgccag ttgctacaac gtaaatgcag cctgctggaa agctgggccg
     5281 cctcgctggg tgtggaagtt agcttcttcc tgattgatga aaaccgtttt cgccacaacg
     5341 agagtggcag tctggggggc gaagattgtg gctccaccca gcatatactg ctgcttgatg
     5401 aattttatcg taccgccgtg cgtctcgccg gtaagcgtat tctgtggaat atggtgccgt
     5461 gcgacgaaga agagcattac gatgactacg taatgacgct ctacgcacaa ggcgtgctga
     5521 cgccaaatga atggctggat ctcggtggct taagctcgct ttctgctgaa gagtactttg
     5581 gtgccagcct ttggcagctc tacaagagta tcgattcccc atacaaagcg gtactgaaaa
     5641 cactgctgct ggaagcctat tcctgggaat acccgaaccc acgtctgctg gcgaaagata
     5701 tcaaacagcg tttgcacgac ggcgagattg tatcgtttgg tctcgatcca tactgcatga
     5761 tgctggagcg tgttactgaa tacctgacgg cgattgaaga ttttacccgt ctggatttag
     5821 tacgtcgctg cttctattta aaagtgtgcg aaaagctcag ccgtgaacgc gcctgcgtag
     5881 gctggcgtcg cgcagtgttg agccagttag tgagcgagtg gggttgggac gaagctcgtc
     5941 tggcaatgct cgataaccgt gctaactgga agattgatca ggtgcgtgag gcgcacaacg
     6001 agctgctcga cgcgatgatg cagagctacc gtaatctgat ccgctttgcg cgtcgcaata
     6061 accttagcgt ctccgccagt ccgcaggata tcggcgtgct gacgcgtaag ctgtatgccg
     6121 cgtttgaagc attaccaggt aaagtgacgc tggtaaaccc gcagatttcg cccgatctct
     6181 cggaaccgaa tctgaccttt atttatgtgc cgccgggccg ggctaaccgt tcaggttggt
     6241 atctgtataa ccgcgcgcca aatattgagt cgatcatcag ccatcagccg ctggaatata
     6301 accgttacct gaataaactg gtggcgtggg catggtttaa cggcctgctg acctcgcgca
     6361 cccgcttgta tattaaaggt aacggcattg tcgatttgcc taagttgcag gagatggtcg
     6421 ccgacgtgtc gcaccatttc ccgctgcgct tacctgcacc gacaccgaag gcgctctaca
     6481 gtccgtgtga gatccgccat ctggcgatta tcgttaacct ggaatatgac ccgacagctg
     6541 cgttccgcaa tcaggtggtg catttcgatt tccgtaagct ggatgtcttc agctttggcg
     6601 agaatcaaaa ttgcctggta ggtagcgttg acctgctgta ccgcaactcg tggaacgaag
     6661 tgcgtacgct gcacttcaac ggcgagcaat cgatgatcga agccctgaaa actattctcg
     6721 gcaaaatgca tcaggacgcc gcaccgccag atagcgtgga agtcttctgt tatagtcagc
     6781 atctgcgcgg cttaatccgt actcgcgtgc agcaactggt ttctgagtgt attgaactgc
     6841 gtctttccag cacccgccag gaaaccggac gcttcaaggc gctgcgcgtt tctggtcaaa
     6901 cctgggggtt gttcttcgaa cgcctgaatg tatcggtaca gaaactggaa aacgccatcg
     6961 agttttatgg cgcgatttcg cataacaaac tgcacggcct gtcagtgcag gttgaaacca
     7021 atcacgtcaa attaccggcg gtggtggacg gctttgccag cgaagggatc atccagttct
     7081 ttttcgaaga aacgcaagac gagaatggct ttaatatcta cattctcgac gaaagcaacc
     7141 gggttgaggt gtatcaccac tgcgaaggca gcaaagagga gctggtacgt gacgtcagtc
     7201 gcttctactc gtcatcgcat gaccgtttta cctacggctc aagcttcatc aacttcaacc
     7261 tgccgcagtt ctatcagatt gtgaagattg atggtcgtga acaggtgatt ccgttccgca
     7321 caaaatctat cggtaacatg ccgcctgcca atcaggatca cgatacgccg ctattacagc
     7381 agtatttttc gtgatgaacg tgccggaaag cgaggcttat ccggcatgca atcttagcgg
     7441 aaactgactg tttcacccgc ctgctgcgtc gccgcctgtt ccagcaaatc ccagaaggtt
     7501 tcgccgctgc gatcgcaaat ccactcatcg cctttcagat caaaatggta gccgccttgt
     7561 ttggttgcca gccatacctg gtgcagcggt tcctggcggt tgataatgat tttgctgcca
     7621 ttctcaaagg taatggtcag cacgccgccg ttgatttcgc agtcgatatc gctgtcgcca
     7681 tcccagtcgt ccagacgctc ttcaatggtc agccacagtt gatcagccag gcgatgaaat
     7741 tcactgtcgt tcattgttgt atcctgtttt taagtgatgg cggcagtata gcggcatggg
     7801 gtcagggctt caaagtttgc acctctgcgg ctgcgttacg gcatgattca tccgtcaacg
     7861 gaataataat gtctctgttg tgaagttcgc gcacattctc ttctctgacg cgtgttttcg
     7921 ccgggcaggg ggagggtgta atccttacct ctgtatcggt ccatgtataa acattgcgat
     7981 cgtcataagc gaagcgatcg tttttagtta gcggaccgtt aactggaata atttgcacgc
     8041 tctttgaccg taattggcgg ataaaataag catgctgttt gccgctatta aatgagtgat
     8101 caatgaccag gtctggcgta tccagcggga ttatctcaag ctggtcttca ccaaatgtcg
     8161 ataattttgt tacatacaac cgatctttat attgcgcaat attgccagta atcagatgaa
     8221 attgttcagg ttcaatacct ggtaggtcta tccacttgct ccactgacgg tcgggcgtga
     8281 gctgtttacg ccagcgaacg cgactttccc ctacttcaaa agagacgtca tcatctacgg
     8341 gtatcgcttt gccagacaat tgcccaaagg gataacgatg cagaccctta ttgtcgcgaa
     8401 aaaccagcag tgaatgaggt atccagcgaa ttatctcaaa tgagtctgca ttcgctgtaa
     8461 ggtgaacggc atttaaataa atattctgcc cactgcggat aagcagatca ttggaataca
     8521 aatttctgtt tacactcata agacgtggat taatacccca ccatcttttt tctaatacag
     8581 taacattatt gctgctcccc tgacgtcgac cggacaaata gagattttta ctatccacca
     8641 gcgtggtaga gttaccttcg atagctttta gtgtcgccag atcaacacgt gacgcgccgc
     8701 tattactttc ggtgcgctgt ccatcaaaat agaggctata tttatcgacc gcgaagcggc
     8761 ccatagcctg aaagctggca atatcgacag tgggtgttcc cggtggattg ctaacgattt
     8821 tgccacgcca gagaataacg cggccgtctg tcacccagtg gtattcctca ttttttatgc
     8881 ttatttcatc agtgggtata tagccttcca ctaactcccg ctccccggat atatttgtca
     8941 caaggcgcaa atcggctctg ttcatcttcg gaaattgggc aatgattttc tcatcctccc
     9001 cataaccttt accgtaagac ataacaccgt gatcattgta atagtcgaac cctatggcct
     9061 gtgcagcagg tgtcagaaat gtgtgcccca acaggatgag tgcaactgtg attatttgac
     9121 ccttcatttt gtcgcctcat tgcgcggggc aggaataaat tcgccgtcat cggtaatttt
     9181 tccgtccaga tatcccgttt tgtaatttac cccgtagcca tatcccgaat gttgttcctt
     9241 ccagattaag cgaacttgtt tcggatcagc ggtgcggtat cgccagagct gcgcgccatc
     9301 aaagctgtaa acgtagcggt tatcgcgggc ataccatctg cgcaaaaagg gatggtcatt
     9361 aagatgggct tcgcgttcat ccggagaacg gttatccagt aagagcagcg gtccgtcaga
     9421 ttcaaacacg tgtaaaccgg aatggtatgg ccacccttcg gcacgcgtaa gtaaatagcc
     9481 gtggcgtttc ccggcgttga aacgtttgtt gataataaga ttgggatcat caagtttcac
     9541 gatctccagc ttgcggtcac cagactctgt ttcgatgatg gtataaagcg agtcctgata
     9601 ctgtgcgaca gcatcactga tggggtgaaa ctgttccgga tcgagtcccg gtaattcttc
     9661 ctgtttacag tcaggcccct gacgccagaa cactttcttt tccagcagag aaaaatccag
     9721 acagtgcttt gttaaatcct catcccacgc cagattttcc ttgtcgagga ctttacgctg
     9781 tagcccgttt ttatcacgcc aggtcaagag tgagccgggc atccagcgca cgacggaaaa
     9841 ggtgtccggg tcggcaacaa gctgttcacc gttgatcagg attttggtcc gtgtacgagc
     9901 cagggtatcc caggggccga atggtacggc aacgcagggg ttaaacgtgg tggaaaactt
     9961 tcctcgctga tcccaggact tctgcgccag cacacggaaa ctctcggggt cagcgaggcg
    10021 atgcccattg atatacaaga aattagcgtc acgtaatatc agccccagta agtcaggagc
    10081 ccagggaggc cggaattcca cctgatgcag cgttttgata tctaagctat ttctcccacc
    10141 attgtcatcg gtgcgcttgc cttcaaaata gagactgtgt ttatctgcag caaaatcacc
    10201 ccatgccttg aacgaggcga catcgacggg aggtgtgccg ggagggtttt gcacaacttc
    10261 acccgcgtag aggatattgc gaccatcgct ataccaggaa taatgaggat accaggcctg
    10321 tgcgccctca tagaggtagt cggttgtcaa atcagtctga ggcggttctt tatcccattc
    10381 gtctagtgtt gtcgggtcta ttaataaatt gggtaaccga cgcaaactct tgtaattcac
    10441 gtcaggtaga gaaagatttg tttctttata agggcgcagg tacaccacgc cttttccctg
    10501 tttctggtaa atattatgtt caggtgcgga aagccgacat ccccatgccg atgataagac
    10561 caatgaacaa gccaacaaac aaaccacatt gcgatagtgc ataaagccat cctggcgcga
    10621 ggtgccgatc acgaaactat cagcaaaaca taaatcccta cgagtaagcg ttatactcgc
    10681 agcatttcct cacttttcag acttcataaa gagtcgctaa acgcttgctt ttacgccttc
    10741 tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata atgaaaaacg
    10801 tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc ggtctgaaag
    10861 gtccgctcta tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa ccggtagaga
    10921 cgcaaacgca atccacggtg ccggataaaa acgaccgcgc cactggcgat ggtccatccc
    10981 aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg atgcagttct
    11041 cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg cagaatgtct
    11101 ttttttcacc ggagctgatt cgtcgcctgg cggatcggca cctgggggta gggtttgacc
    11161 aactgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat cgcattttca
    11221 atgctgatgg cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt gcccgttttg
    11281 tgcgcctgaa agggctgacc aataagcgcg atatccgcgt cagcaccgcc aacgggcgga
    11341 tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa cccaacttcg
    11401 aaccttccgc tgtgccgttt cgcgctaaca aagcggaaaa gacctacatt atgcgcgccg
    11461 ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc gtgattcagg
    11521 tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa agccacgagc
    11581 gttttccgga gcgagccaat atcggtttta tgcaagtggt taagcgcgag catattcgtt
    11641 tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc gcatgtgcgg
    11701 cggtggcggt agggattcag caaggtttgc tggccgaaga agtacgcgtg gaactccccg
    11761 gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg actggcccgg
    11821 cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag aactgcagga
    11881 aacactcacg gagcttgatg accgggcggt tgtcgattat ctgattaaaa atcctgagtt
    11941 ttttatccgc aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg tacgcggcac
    12001 cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat catattcatg ttctggaaga
    12061 gaacatggcg ctgttgatgg aacaggctat cgccaacgaa ggcctgtttt atcgcctact
    12121 ctacctgcag cgcagtctca ccgccgccag cagtctcgac gatatgctga tgcgctttca
    12181 ccgctgggcg cgcgatctcg gcctggcagg tgcgagtctg cgcctgtttc cggatcgctg
    12241 gcgcttaggt gcgccgtcga accacactca tctggcatta agccgtcagt ctttcgaacc
    12301 gctgcgtatt cagcgtttgg ggcaggaaca gcactatctt gggccgctta acggaccaga
    12361 gctgctggtg gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt cgatgctggg
    12421 aagcgatgct gatttgggtg tcgtgctgtt taccagtcgc gatgccagtc actatcaaca
    12481 agggcaggga acgcagttac ttcatgaaat tgcgctgatg ttgccggagc ttctggagcg
    12541 ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctacct acgttatctg
    12601 agcgtggagc gtcagcttag cccgataacc ctacttaact accagcgtca gcttgaggcg
    12661 atcatcaatt ttgccagcga aaacggcctg caaagctggc aacaatgcga tgcagcgatg
    12721 gtacgcaatt ttgctgtacg cagtcgccgt aaagggctgg gagcagcaag cctcgcgtta
    12781 cggctttctg cgctacgtag cttttttgac tggctggtca gccagaacga actcaaagct
    12841 aacccggcga aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa aaacatcgac
    12901 gtcgacgata tgaatcggct gctggatatt gatatcaacg atcccctcgc tgtacgcgac
    12961 cgtgcaatgc tggaagtgat gtacggcgcg ggtctgcgtc tttccgagct ggtggggctg
    13021 gatatcaaac acctcgacct ggagtctggt gaagtgtggg taatggggaa aggcagcaaa
    13081 gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg gcttgatttg
    13141 cgcgacctgt ttggtagcga agacgacgcg ctttttctgt cgaaactggg caagcgtatc
    13201 tccgcgcgta atgtgcagaa acgctttgcc gaatggggca taaaacaagg gctgaataat
    13261 cacgttcacc cgcataaatt acgtcactcg ttcgctacgc atatgctgga gtcgagcggc
    13321 gatcttcgtg gtgtgcagga gctgctgggt catgccaacc tctccaccac gcaaatctat
    13381 actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc acgcgccaaa
    13441 cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc acctttgacc
    13501 tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcga gaggcgctta
    13561 cctttgtgca aaattatcat ccggcgctgc gcagcttcca gaatgaagat ctgcaacgcc
    13621 tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg cgttggcgtt
    13681 ttcgttcgat tgaacaagcg atgctcgacg ccgggctgag tgccgaagaa gccagtgcag
    13741 gcgcacacgc agcaatgatt aactttgcca aatggcgcag ccgaatcgac gtcccgcagc
    13801 aaactcacga caccttaaaa cagctggcga agaaatggcc gctggtggcg atcaccaacg
    13861 gtaacgccca gccggaactg tttggtttgg gggattattt tgagtttgtg ctgcgcgctg
    13921 gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt tctggctgcg gaaaaactca
    13981 acgtgccgat cggcgagatc ttacatgttg gggacgatct caccactgac gtgggtgggg
    14041 caattcgcag cggaatgcag gcctgttgga tcagaccgga aaatggcgat ctgatgcaaa
    14101 cctgggacag ccgtttactg ccgcatctgg aaatttcccg gttggcatct ctgacctcgc
    14161 tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg ttgcgcttct
    14221 ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc tcgacagcct
    14281 taatgacaaa cagcgcgaag cggtggccgc gccacgcagc aaccttctgg tgctggcggg
    14341 cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga tgagcgtgga
    14401 aaactgctcg ccatactcga ttatggcggt gacgtttacc aacaaagcgg cggcggagat
    14461 gcgtcatcgt atcgggcaac tgatgggcac cagccagggc ggcatgtggg tcggcacctt
    14521 ccacgggctg gcgcaccgtt tgctgcgtgc gcaccatatg gacgccaatc tgccgcagga
    14581 tttccagatc ctcgacagtg aagaccagct acgcctgctt aagcgtctga tcaaagccat
    14641 gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca acagccagaa
    14701 agatgaaggc ctgcgtccgc atcatattca aagctacggt aatccggtgg agcagacctg
    14761 gcagaaggtg tatcaggcgt atcaggaagc gtgtgaccgc gcgggtctgg tggacttcgc
    14821 cgagctgctg ctgcgcgccc acgaactgtg gcttaacaag ccgcatatcc tgcaacacta
    14881 ccgcgaacgt tttaccaaca tcctggtgga cgaattccag gataccaaca acattcagta
    14941 cgcgtggatc cgcctgctgg cgggcgatac cggcaaagtg atgatcgttg gcgatgacga
    15001 ccagtcaatc tacggctggc gcggggcgca ggtggagaat attcagcgtt tccttaatga
    15061 tttccccggt gctgaaacta tccgtctgga gcagaactac cgctctacca gcaatattct
    15121 gagcgccgcg aacgccctga ttgaaaacaa caacgggcgt ctgggtaaaa aactgtggac
    15181 cgatggcgcg gacggtgagc cgatttccct ctattgcgct tttaacgaac tcgacgaagc
    15241 gcgttttgtg gttaatcgca tcaaaacctg gcaggacaac ggcggggcgc ttgccgagtg
    15301 cgccattctc taccgcagca acgcccagtc gcgtgtactg gaagaggcct tattacaggc
    15361 gagtatgccg taccgtattt acggcgggat gcgattcttc gaacgccagg aaatcaaaga
    15421 tgcgctctcg tatctgcgcc tgattgccaa ccgcaacgac gacgcggcct ttgagcgtgt
    15481 ggtgaatacg ccaacgcggg gtattggtga ccggacgctg gacgtggtac gtcagacatc
    15541 gcgcgatcgc cagttaacac tctggcaggc atgtcgtgag ctgttgcagg aaaaagcgct
    15601 cgccgggcgt gctgccagcg ccttacagag gtttatggaa ttaatcgacg ccttagcgca
    15661 agaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag actccggcct
    15721 gcgcaccatg tacgagcagg agaagggtga aaaaggccag acgcgtatcg aaaacttaga
    15781 ggaactggtg acggcaacgc gccagttcag ctacaacgaa gaagatgaag atttaatgcc
    15841 gctgcaggcg ttcctctccc atgcggcact ggaagcgggc gagggacagg cggatacctg
    15901 gcaggacgcg gtgcagttga tgacgctaca ctcggcgaaa gggctggagt tcccgcaggt
    15961 gtttatcgtc ggtatggaag agggcatgtt cccaagccag atgtcgctgg atgaaggcgg
    16021 tcgtctggaa gaagaacgcc gtctggccta cgttggcgta acccgtgcga tgcagaaact
    16081 gacgctgacc tacgcggaaa ctcgccgtct gtatggcaaa gaggtttacc atcgcccgtc
    16141 gcgctttatc ggtgagttgc cggaagagtg tgtggaagag gtgcgcctac gcgccacggt
    16201 aagccgcccg gtcagccatc agcgtatggg tacgccgatg gtcgagaacg acagcggcta
    16261 caagctcggc cagcgtgtac gccacgctaa gtttggtgaa ggcactatcg tcaatatgga
    16321 aggcagcggc gagcatagcc gtttgcaggt ggcattccag ggccagggaa tcaaatggct
    16381 ggtggcggct tacgcccggc tggagacggt ataacgttgc gggatgcggt gcgccacacc
    16441 gcatccggca tcagcacctt acttatcaca tttcttcaca tcaatattcc agtcagcacc
    16501 agacgcaacc tgatgtttct gcgcgaaatt atccatattc agcgccacgg aaacattcaa
    16561 taagctgttc agatacacca tcggctgtcc ttccggcaca tcgccgaagc tggcgacata
    16621 cggcattttc ccttcgtatt ttttctcgga accttcggaa atggtcacac ataacgtatc
    16681 gttgagtttg attcctgcct gattcagcaa ctcgtcgctg atattgctcc agacgttacc
    16741 gtactgaata tcgagaatcg ggatattgcc cttcacttca ccttttgtcg ctttcgcttt
    16801 ctgataaggc agttcgacaa cttttgccgg aagctctggc cccacctgtt cgaacgtgat
    16861 cgctccggaa gccaggcgtg caccggtgta ggcatataca tcgcgaccgt ggaaggtata
    16921 tgacttttcg gaacctttca ggcggttggt tttttcatcg atttgccgga cgctctcaat
    16981 acccagcgat tccgccacca aagtgagcgt accgttatct ggtgaaacga agtattgacc
    17041 gtttttggtt ttcagcacta ccgatttacg gtctgtacca acaccaggat cgaccacact
    17101 gacaaatacc gaaccttgcg gccagtaact ggcggtctga tacaggcggt aagcgccttc
    17161 ccagatgtta tacggtggaa tttcgtgtgt taaatcaaat attttaagat tatggtcaac
    17221 accgaatgcg acccctttca tcgccgagac ggcaccatct ttcaggctaa aatcagtttg
    17281 taaaatcaaa gcgttatccg cataagcgga tgatgatccc agacacgtta ataaaaataa
    17341 cgttgcgagc gtttttttca tttttgtttc ccttcattta tctgatgatt ttccagctgt
    17401 gtagccagcc ccagtttgtc tggcctcctc gagtgtacaa tgcttaacct ttccgctcga
    17461 cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca
    17521 tatgtgaaat cgtgccatca agataaagca gctgctcaac gttcagtttc gctttggcat
    17581 aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcattcccat
    17641 gtttattaat cccaacaccg ttacgaattt tacgtgaggc gacgttggga tgaatacgcg
    17701 gattaattac actgttttcc atcaacattg gccctgactg caccgcaaac tgaatctctt
    17761 tactggtttt gaaggcatcc agacgaacga tgccgacttt atctcccgcg acataaaaca
    17821 cgccgccagg acggataaag aaattccctt cacctgaagc gagatttaac gccaccttct
    17881 gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag atgccgccgt
    17941 tcatcgccat ctgcacctga ccctgactat tcatatccgc cagcagagca tgtaaagttc
    18001 cccacgcttc gccattggct ttttgccagt acattttcac ccgctctgtt tgaggattaa
    18061 cggtatacgc ctgtacggtc agcgtcggat ctgagagtgc gcaatcatca gcggcaacag
    18121 caaacaaggg aagtaaggtg agggtgagaa aaatccgttt gagattcaag gtgatcattc
    18181 ctttaccaat gagtagccga tgcgccatta taggtcctgg atgtgggatt tttttatcct
    18241 gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct gtggtgttct
    18301 gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca ctctaagagg
    18361 acattcgcct tggacacacc cagtagatac tggctcacta tcctgtcatc caggatcaac
    18421 tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct caatttttcc
    18481 cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg tgtgcccacc
    18541 gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat ttcaactgga
    18601 aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa atgcagtatg
    18661 gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg aacttggcca
    18721 gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt tctttgaaga
    18781 cgacgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag atcacgccgg
    18841 taactccact gtggcattta ccatccgtga tggtcgtctg tttactctgc gtgagcgtga
    18901 actgcccgct tttcgtctgt atcgtatgcg tgcccgtagc cagtcgatgg tagacggtaa
    18961 cgcctacgag ttgctgctgg atttgttcga aaccaaaatc gaacagttgg cagatgaaat
    19021 tgaaaatatc tatagcgacc tggagcaatt gagccgggtg attatggaag ggcatcaggg
    19081 cgatgagtac gacgaggcgc tctccactct ggcggaactg gaagatatcg gctggaaagt
    19141 tcgcctgtgt ctgatggata cccagcgcgc tctcaacttc ctggtacgta aagcacgctt
    19201 accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat ccctgctgcc
    19261 gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcaa tgggttttat
    19321 caacatcgag cagaaccgca tcatcaaaat tttctcggtg gtatccgtcg tgttcctgcc
    19381 gccaacgctg gttgcttcca gctacgggat gaactttgag tttatgccag aactgaagtg
    19441 gagtttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg caccgtatct
    19501 gtactttaag cggaagaact ggttgtaata tgagtgccgg ataaagctat ttttttatcc
    19561 ggcttatttc taaaaattgt cttttttaaa gtcatgcgga t
//
LOCUS       DAZUGK010000082        19224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZUGK010000082 DAZUGK010000000
VERSION     DAZUGK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.082"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            288..593
                     /locus_tag="ACHY5W_002482"
     CDS             288..593
                     /locus_tag="ACHY5W_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447367.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            700..1344
                     /gene="gfcB"
                     /locus_tag="ACHY5W_002483"
     CDS             700..1344
                     /gene="gfcB"
                     /locus_tag="ACHY5W_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFS4447368.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALTIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1341..2087
                     /gene="gfcC"
                     /locus_tag="ACHY5W_002484"
     CDS             1341..2087
                     /gene="gfcC"
                     /locus_tag="ACHY5W_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS4447369.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2087..4183
                     /gene="gfcD"
                     /locus_tag="ACHY5W_002485"
     CDS             2087..4183
                     /gene="gfcD"
                     /locus_tag="ACHY5W_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS4447370.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWDDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQARHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4229..5368
                     /gene="gfcE"
                     /locus_tag="ACHY5W_002486"
     CDS             4229..5368
                     /gene="gfcE"
                     /locus_tag="ACHY5W_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFS4447371.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5356..5802
                     /gene="etp"
                     /locus_tag="ACHY5W_002487"
                     /gene_synonym="yccY"
     CDS             5356..5802
                     /gene="etp"
                     /locus_tag="ACHY5W_002487"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFS4447372.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5822..8002
                     /gene="etk"
                     /locus_tag="ACHY5W_002488"
     CDS             5822..8002
                     /gene="etk"
                     /locus_tag="ACHY5W_002488"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFS4447373.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8122..9420)
                     /gene="appA"
                     /locus_tag="ACHY5W_002489"
     CDS             complement(8122..9420)
                     /gene="appA"
                     /locus_tag="ACHY5W_002489"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFS4447374.1"
                     /translation="MKAILLPFLSLLIPLTPQSTFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9500..9592)
                     /gene="cbdX"
                     /locus_tag="ACHY5W_002490"
     CDS             complement(9500..9592)
                     /gene="cbdX"
                     /locus_tag="ACHY5W_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFS4447375.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9605..10741)
                     /gene="appB"
                     /locus_tag="ACHY5W_002491"
     CDS             complement(9605..10741)
                     /gene="appB"
                     /locus_tag="ACHY5W_002491"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS4447376.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10753..12297)
                     /gene="appC"
                     /locus_tag="ACHY5W_002492"
     CDS             complement(10753..12297)
                     /gene="appC"
                     /locus_tag="ACHY5W_002492"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFS4447377.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTRQQG"
     gene            complement(12431..13288)
                     /gene="hyaF"
                     /locus_tag="ACHY5W_002493"
     CDS             complement(12431..13288)
                     /gene="hyaF"
                     /locus_tag="ACHY5W_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFS4447378.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHKLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13285..13683)
                     /gene="hyaE"
                     /locus_tag="ACHY5W_002494"
     CDS             complement(13285..13683)
                     /gene="hyaE"
                     /locus_tag="ACHY5W_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFS4447379.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13680..14267)
                     /gene="hyaD"
                     /locus_tag="ACHY5W_002495"
     CDS             complement(13680..14267)
                     /gene="hyaD"
                     /locus_tag="ACHY5W_002495"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFS4447380.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14264..14971)
                     /gene="hyaC"
                     /locus_tag="ACHY5W_002496"
     CDS             complement(14264..14971)
                     /gene="hyaC"
                     /locus_tag="ACHY5W_002496"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFS4447381.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14990..16783)
                     /gene="hyaB"
                     /locus_tag="ACHY5W_002497"
     CDS             complement(14990..16783)
                     /gene="hyaB"
                     /locus_tag="ACHY5W_002497"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFS4447382.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16780..17898)
                     /gene="hyaA"
                     /locus_tag="ACHY5W_002498"
     CDS             complement(16780..17898)
                     /gene="hyaA"
                     /locus_tag="ACHY5W_002498"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFS4447383.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18324..18411
                     /locus_tag="ACHY5W_002499"
     tRNA            18324..18411
                     /locus_tag="ACHY5W_002499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18358..18360,aa:Ser,seq:tga)
     gene            18494..18898
                     /locus_tag="ACHY5W_002500"
                     /pseudo
     CDS             18494..18898
                     /locus_tag="ACHY5W_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
BASE COUNT         4762 a         5072 c         4926 g         4464 t
ORIGIN      
        1 gttatagtac cacttgaaac tatattgcca acaatagtta atagctttct taacaatttt
       61 cttatcttta atttgtattt tgctaagatt tatcttatgc tcacaagtcg tatttccaga
      121 ggggaatagt taacaatctt atatttccct ctgcgcgcct ttctcacccg ctgtatatag
      181 tgcaagtggt atgaggaaag ggagatttca caaggatgtt atactcaaag tagacaatcc
      241 tgatctcaag cgtacgtatt gtccatgcga tgaataaagg aaaagttatg aaacacaagc
      301 tttctgcaat ccttatggcc ttcatgttga cgacgccagc tgcgtttgct gcccctgaag
      361 cagcaaacgg gaccgaggca acgacgggta ctactggcac cacaacgacc actaccggtg
      421 caaccacgac ggctgctacc actggtggcg tggctgctgg cgctgtcggc acggcgacag
      481 ttgtcggtgt ggcaacggct gttggtgtgg cgacgttggc agtagttgca gcaaatgaca
      541 gtggcgatgg cggttctcat aacacctcaa ccaccacgtc tactacgcgc taattctgct
      601 taaacgcgtc gaaattaaag ttaaataata cccggttgcg atattcgtac cgggctttag
      661 agatttgcat ttttttaatt aacctttcaa ggattaaagg tgcgccctct tattttatcg
      721 attttcgcac tatttcttgc gggatgtacg cacagccagc aaagtatggt cgatacattt
      781 cgcgccagcc ttttcgataa tcaggatatc accgtagcgg atcagcagat ccaggcgttg
      841 ccttattcca cgatgtattt acgccttaat gaagggcaac gaatctttgt ggtactggga
      901 tatatagaac aagaacaaag caaatggtta tcccaggata acgccatgct ggttactcat
      961 aatggacgtc ttttaaagac cgtcaaactt aataataatc tgctggaagt gacaaattcc
     1021 gggcaggacc ctctgcgtaa cgcgctgaca ataaaagacg gcagccgctg gacgcgcgat
     1081 attctctgga gtgaagacaa ccattttcgc tctgcgaccc tgagttccac tttttccttt
     1141 gctggattag agacgctgca tattgcgggt cgcgatgtgc tgtgtaatgt ctggcaggaa
     1201 gaggtgactt ccacgctacc agaaaaacag tggcaaaaca cattctgggt cgattcggct
     1261 actggccagg ttcgtcaaag tcggcaaatg ttaggcgcag gggttattcc cgtagaaatg
     1321 acgtttctta aacccgcacc atgaataaat tacagtcgta tttcattgcc agcgtacttt
     1381 acgtaatgac accccatgcc tttgcgcaag gaacggtgac tatttatctg cctggcgaac
     1441 aacagacact ttccgttggc cccgtggaga atgttgccca gttggtgaca caaccgcaac
     1501 tgcgcgatcg cctctggtgg cctggtgccc tgctgaccga ctccgcggtg aaagctaaag
     1561 cgcttaaaga ctaccaacat gtcatggcac agcttgcttc ctgggaagca gaagctgacg
     1621 acgatgtagc ggcaacgatt aaatccgtgc gccagcagtt actcaatctc aatatcactg
     1681 gtcgcttacc ggtcaaactg gaccctgatt ttgtacgagt ggatgagaac agcaatcccc
     1741 cgctggtcgg ggactatacg ctctataccg tacagcgtcc ggtgactatt actctgctgg
     1801 gtgcggtttc tggggctggg caattgccct ggcaggcagg ccgtagcgtt accgattatc
     1861 tgcaagatca cccccgcctt gcaggcgcgg acaagaataa cgttatggtg attacgcctg
     1921 agggtgagac tgtcgtcgcc cctgttgccc tgtggaataa aagacatgtg gaaccaccac
     1981 ctggcagcca gctttggtta ggcttttctg cgcatgtact gcccgaaaaa tatgctgatt
     2041 taaatgacca gattgtgtct gttttaacgc aacgtgttcc tgactgatga agaagaattc
     2101 ttatctttta agctgcctgg ccattgccgt ctccagtgcc tgtcatgctg aagtattaac
     2161 ctacccggat ccgctgggtt cgtcgcaatc ggactttggc ggcacaggat tgttgcagat
     2221 gccaaatgcg cgcatcgcac cggaaggtga attcagcgtc aactaccggg ataacgatca
     2281 ataccgtttc tactccacct ccgtggcgct gttcccatgg ctggaaggca ccattcgtta
     2341 tacggatgtg cgcacacgta aatatagcca gtgggacgat ttcagcggcg atcagtcata
     2401 caaagacaaa tcattcgatt ttaaacttcg cctgtgggaa gaaggttact ggctaccgca
     2461 agtggcgttt ggtaaacgcg atattgctgg tacgggtctg tttgacggtg agtatttggt
     2521 ggccagcaag caagcggggc catttgattt caccctcggg atggcatggg gctacgccgg
     2581 taatgcgggc aatattacca acccgttttg ccgggtgagc gataaatatt gtcatcgcgc
     2641 agagtctcac gatgcgggcg atatcagctt tagcgatatc tttcgtggcc cggcttccat
     2701 ctttggcggc attgagtatc aaacgccgtg gaatcccctg cgtctgaaac tcgaatacga
     2761 cggcaacaat taccagaatg atttcgctgg caaactgcct caggcaagac atttcaacgt
     2821 cggtgcagtt tatcgcgctg ccagctgggc agacctcaac ctgagttatg aacgcggtaa
     2881 cacgttgatg tttggtttca ctttacggac caatttcaac gatctgcgcc ctgccctgcg
     2941 cgatacgcca aaaccggcat atcaacctgc gcctgaatct gaaggattgc agtacaccac
     3001 ggtagcaaac caacttaccg ccctgaagta taacgcgggc tttgacgcgc cagaaattca
     3061 gctacgcgat aagacgctgt atatgtctgg tcagcaatac aaataccgtg actctcgtga
     3121 agcggtcgat cgtgccaacc ggattctggt gaataacctg ccgcaaggcg ttgagaagat
     3181 tagcgtgacg caaaagcgcg agcatatggc gatggtgact accgaaaccg acgtagccag
     3241 cctgcgcaaa cagctggctg gtacagcgcc tggtaaatca gagcaactgc aacaacaacg
     3301 tgttgaagca gaagatcttt ctgcctttgg tcggggctac cgtattcgtg aagatcgctt
     3361 tagctactct ttcaacccaa cactttcaca atcgctgggc ggcccggaag atttctatat
     3421 gttccagttg gggctgatgt ccagcgcccg ctactggttt actgaccacc tgctgcttga
     3481 tggcggtatt ttcaccaata tttacaataa ctacgacaag tttaagtctt cgctattacc
     3541 cgcggactct accctgcccc gcgtgcgcac gcatatccgt gattacgttc gcaatgacgt
     3601 ttatctcaac aacttgcagg cgaactactt tgccgactta ggcaatggtt tctatggcca
     3661 ggtgtatggc ggttatctgg aaacgatgta cgccggtgtc ggttccgagc tgctttatcg
     3721 cccgctagat gccagctggg cgctgggtgt ggacgttaac tacgtgaagc aacgtgactg
     3781 ggacaacatg atgcgcttca ccgattattc cacgccaact ggtttcgtga cggcttactg
     3841 gaacccgccg acgctcaatg gcgtactgat gaaacttagc gttgggcaat atctggcaaa
     3901 agataaaggc gcaacgatcg acgtcgccaa acgctttgac agcggcgtgg cggtaggggt
     3961 atgggcggca atcagtaacg tatctaaaga tgactacggc gaaggcggct ttagtaaagg
     4021 tttttatatc tcgattccgt tcgacttgat gaccattgga cctaaccgca accgcgcggt
     4081 ggtttcgtgg acaccattga cgcgtgatgg tggacaaatg ctgtcacgca aataccagct
     4141 ctatccaatg acggcagagc gagaagtacc ggttggacaa taacctgacg taattcataa
     4201 cattacattg actgataaat ataaaccaat gaaaaagaac atttttaagt tttcggtact
     4261 gacattggct gttctgtctt tgacagcatg tacccttgtt cctggtcaga atttatcgac
     4321 cagtaacaag gatgtcattg aactacctga caatcagtat gacctggaca aaatggtcaa
     4381 tatctatccc gtcacgccag gattgattga tcagctgcgc gccaaaccga ttatgtcgca
     4441 ggctaacccg gagctggagc agcaaatcgc gaactatgaa tatcgtatcg gcattggtga
     4501 tgtgttgatg gttacggtct gggatcaccc ggaactaacc acccccgcag ggcaataccg
     4561 tagcgccagt gataccggca actgggttaa cgccgatggt gcgattttct acccctatat
     4621 tggtcgcctg aaagtggccg ggaaaacact tacccaggta cgcaacgaga ttacagcgcg
     4681 tctggattca gtcattgaaa gcccgcaagt cgatgtcagc gtcgcggcgt tccgctcgca
     4741 aaaagcgtac gtcaccggtg aagtcagcaa atcgggtcag cagccgatta ccaatattcc
     4801 tctgaccatc atggatgcga ttaacgccgc gggggggctg accgcagatg cagactggcg
     4861 caatgtggtg ctcacccaga atggcgtaaa gaccaaagtg aacctctatg cgctgatgca
     4921 gcgtggcgac ttgcgccaga acaaactgct gcacccgggc gatattttat ttattccgcg
     4981 caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat
     5041 ggaccgcagc ggtatgaccc tggcggaagc gctgggtaac gctgagggta tgaatcagga
     5101 tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa
     5161 aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga
     5221 attccagctt gaaccttacg acattgttta tgtcactacc gcgccgctgg ctcgttggaa
     5281 ccgtgtgatt tctctgctgg taccgacgat ttccggggtg catgacctga cagagacttc
     5341 acggtggatt caaacatggc ccaactaaaa tttaactcaa tcctggtggt ttgtaccggc
     5401 aacatttgcc gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc aggcgtaaaa
     5461 gtcaaatcgg caggtgttca tggtctggta aaacaccctg cagatgcgac agcagcagat
     5521 gttgccgcta atcatggcgt atctcttgaa ggacatgccg gacgtaagct cactgctgag
     5581 atggcacgaa attacgatct gattctggca atggagtcgg aacatattgc tcaggttacc
     5641 gcaatcgctc ccgaggtccg cgggaagaca atgctgtttg ggcaatggct ggaacagaaa
     5701 gagatcccgg atccctatcg taaaagtcag gacgcatttg aacatgtcta cggtatgttg
     5761 gagcgcgcca gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt tataaaatac
     5821 gatgacaact aaaaatatga atacgccacc aggcagcact caggaaaatg agatcgatct
     5881 gcttcgtctg gtcggcgagt tatgggatca ccgtaagttt attatcagcg tgaccgcgtt
     5941 attcacgctg atcgctgtcg cttactcgct gttaagcaca ccaatttatc aggcagatac
     6001 tctggtccag gttgagcaaa aacagggcaa cgccattctc agcggcctga gtgatatgat
     6061 ccctaactca tcgcccgagt ctgcaccgga gatccaactg ctgcaatcgc gcatgattct
     6121 cggtaaaacc attgctgaac tgaatctgcg cgacatggtt gagcagaagt attttccgat
     6181 tgtgggtcgc ggctgggcga gattaaccaa agagaaacca ggtgagctgg cgatcagctg
     6241 gatgcatatt ccacaactga atggtcagga tcagcaactg acactcacgg ttggggaaaa
     6301 cggccactat acgctggaag gtgaagagtt caccgtcaat ggtatggtcg gccagcgtct
     6361 ggaaaaagat ggcgttgcgc tgactatcgc ggatattaag gccaaaccag gaacacagtt
     6421 tgtcctgagc caacgtaccg aactggaagc gattaacgcg ttgcaggaaa cctttaccgt
     6481 tagcgaacgc agtaaagaaa gcgggatgct ggaacttacc atgaccggtg atgatcccca
     6541 gttgattacc cgtatactga acagcatcgc taacaactat ttgcaacaga atatcgctcg
     6601 ccaggcggcg caggattcac aaagccttga attcttacag cgccagttgc ctgaagtgcg
     6661 cagcgagctg gaccaggcgg aagaaaaact caacgtttat cgccagcagc gcgattcggt
     6721 tgaccttaac ctggaagcca aagccgttct tgagcagatt gtgaacgttg ataatcaact
     6781 caatgagctg accttccgcg aggcagagat ctcccagctg tataagaaag atcacccaac
     6841 ttatcgtgcg ctgctggaaa aacgccagac gctggagcaa gaacgcaaac gcctgaataa
     6901 gcgggtatcg gctatgcctt ccacccaaca ggaagtgttg cgtttaagtc gtgacgtaga
     6961 agcgggccgt gcggtatatc tgcaattact taaccgccag caggagttga gtatttcgaa
     7021 atccagtgcc attggtaacg tgcggattat cgacccggca gtcactcagc cgcagccagt
     7081 gaaaccgaaa aaagcgttga acgtggtgct tggttttatt cttggcctgt ttatttcggt
     7141 gggtgccgtg ctggcgcgtg cgatgttgcg tcgtggtgta gaagcaccgg aacaactgga
     7201 agagcacggc atcagcgttt atgccaccat cccgatgtcc gagtggctgg ataaacgtac
     7261 ccgtctgcgt aagaaaaatt tattttctaa tcagcagcgc catcgtacta aaaatatccc
     7321 cttcctggcg gtggataacc cggcggattc tgctgtggaa gccgtacgtg cgctacgaac
     7381 cagtctgcat ttcgctatga tggagactga gaataacatt ctgatgatca ccggtgcgac
     7441 gccagacagt ggtaaaacat ttgtcagttc aactctggca gcggtgatcg cccagtccga
     7501 tcaaaaagtg ttatttattg atgccgactt acgccgtggt tattcgcata acctgtttac
     7561 cgtgagtaat gaacatggct tgtcggaata tctggcaggt aaagatgagc tcaacaaagt
     7621 gatccagcat tttggcaaag gaggctttga tgtgattact cgcggtcagg tgccacctaa
     7681 cccgtctgaa ctgctgatgc gcgatcggat gcgtcaatta ctggaatggg cgaacgacca
     7741 ttacgatctg gtgattgtcg atacgccgcc gatgctggcg gtgagcgatg ctgcggtcgt
     7801 ggggcgctct gtcggcacca gcctgctggt tgcgcgtttt ggcctgaaca ccgccaaaga
     7861 ggtgagcttg tcaatgcagc gtctggaaca ggcaggcgtc aatattaaag gcgctatcct
     7921 caatggtgtg attaaacgcg ccagcaccgc ttacagttac ggctataact attacggtta
     7981 tagttactcc gagaaagagt aaaactgaag gtaaagtgac cggatgcggc gtgaacgcct
     8041 tttccggtct acgcattaga aagttcttcg tttgtcatca gcctcagagc attcaggtag
     8101 ctgaatgctc ttttttatgc attacaaact gcacgccggt atgcgtgctt cattcacgat
     8161 ttgcgtaaaa ccggccaacg aacacatgcc ctgcgcattt cgctcttcac atcctgccag
     8221 ggtcagtttc acctctccgg gcggcgtatt taatgacagc ggcgttttat cacgcatctg
     8281 ctgtaaagtc tggaagacca gcgaaacctg aatccactgg ctgttatcgc ttagccgacg
     8341 ccagcgttca aacaccagtt caccacctgg cggcgtgtta tccggctgac ctggaagcgt
     8401 ccagttgagc tccagtgcgc cgccgagatt tgccagatta gtatcgtgtc cggcgataaa
     8461 cagcactgaa gtgggtaatg tcacaccata cgcctgtttt tgcggtggat ggggcgtcaa
     8521 cgctgtcatg atcaaatcca acaacggggt ggcgcggctg cgggcaacct ctggcgtgcg
     8581 ttgtagtaaa taaaattgcg cgttatgcaa acttagcaac gtgttccact ggtgtgaatc
     8641 agtgatcctt ccccaccccg gctccggcat tccctgtgct tgttgcagga gaaatatttc
     8701 cgtcagcatt gatgcgaggc ttaccgcacc ggttaatgag acattgtcgg cgctcacctt
     8761 gagttccgat ggtaatgcct gcgttaatga acagctttcg tcctgtttct cacggttaag
     8821 gcacaagttt gattgcggaa aattaagcac ccgttccagt tcgcgaaacg ccgtttgccg
     8881 atgcccggta aagtcagcaa ttgaccctcc tgccctgctg aggatcgcgt cagtcacgtt
     8941 cgcgttatcc agttggcaaa cgccagtttt tagaggatta aataacggat cgggactgga
     9001 cgtatctgcc tgggtatgta cggttattgc acagtcaggt gccagcccgg cggcgaaggc
     9061 ttcgcctgtt ttacgggtac gctcgtcgac atcagcaata atcgcgacct gaccagactg
     9121 cgggcagccc tttttcgcca gcaatccgtc ggctaccaga cgctggcgtt ggtaatgtcc
     9181 gagataggcg attagctcac caccgcgcgg tgtcagccaa cccagtttta ccggccaggt
     9241 tggccatgcg tctggggtga catcctgcat cagttgcgtg gccttggttg gtgcacgcac
     9301 accatgacga ctgacaatca ccacactttc cagcttcagt tccggctcac tctgagcgaa
     9361 tgtagattgc ggggttaacg gaatcagaag agataaaaat gggagcaaga tcgctttcat
     9421 cgatatgttt ccgcttttca tatccgacat tattgattgc ctgatgcgac gctaatgcgc
     9481 cacatcagac ttacgttcgt taacttttca atcgcgggtc cagccatacc aacacaaggg
     9541 tggagagcga acacatcaac aaaatgccaa cgaaccaaag taaataccac attgttcctg
     9601 ctccttagta cagctcgttt tcgttacggc ggagagtttc tgtcgtcatg cgcccccaca
     9661 ttttgtagta gctccagaga gtgtagagca acacaatggg caaaaatatc agcacgatta
     9721 ccaacataat actcagcgtc agctgactgg aagtgctgtc ccacaacgtc aggctggaga
     9781 ttggactcac gcttgacggc atgacaaagg ggaacagcgt gatgcctgcc gtgaaaatca
     9841 cgccaaattg catcaatgat gccatcaaaa atccccaacc cgggcgacca cgataaatcg
     9901 ccatcaccgt cagcaatggg cagaagaacc ccagtagcgg gaagatccac aaaacgggcg
     9961 actcgacaaa attattcatc caggcaccag gtagcactgc caccagtttc attaacggat
    10021 tggaaggacc gttagcatcc tgggcgagca gtacaaagcc atcaataccg acccacagcc
    10081 agtaacccgc cagcaaaaag cacagcatca ccaacagtgc agcgcgttta gtcgccagct
    10141 gtgaacgcag atgaatcaca ccaacagttt tcagttgtaa ccagacgcca ccttgcaaaa
    10201 tcaccatccc aaggctgagc aatccgcaca ataaagggaa tggcgtcagc agttgccaga
    10261 agctgccgag atactccacg cgtaattgcg gtgtgaaggc aaacggcacg ccgagcaaca
    10321 agttgccgaa ggcgataccg aagactaccg gcggcaccag actgccgatg accagaccgg
    10381 catcccacat tttacgccag cgtgcatcgg cgatttttcc gcgataatca aaggccagcg
    10441 ggcggaagaa cagcgagcac agcaccagga tcatcgccac ataaaagccg gaaaacgccg
    10501 ctgcatacac tctgggccag gcggcaaata atgccccacc tgcgaggatc aaccagacct
    10561 gattgccttc ccagtgcgca ccaacgctat taatcactat ccggcgttca tcatcatcac
    10621 gcgccaccag cggcagcaaa cagccaatcc ccatgtcaaa tccgtcggag atcataaaga
    10681 ccaccaggat cacgccaatc agcagccacc agatgaagcg caatgtttca taatcaaaca
    10741 tgattttctc ctttacccct gttgccgcgt cggttgttca ctctgcatcg cgctcggccc
    10801 cagacgggca tatttctgca tcaggtagac ttcggcgatt aagaacaggg tgtaaagccc
    10861 tacgatcatg atcagtgaga aagccagttg tcctgtggtt aaagcggagt gcgcggagta
    10921 tgtcggtaag atgtcctgta tcgcccacgg ctgacgacca aactcggtca taaaccaccc
    10981 ggcttcaatc gcaatccacg gcaacggcaa actccagagc gccattttca gcacccagcg
    11041 atgctggtcg attttgccac gcagcgtctg gacaagcgca atcagcatca ccagtagcag
    11101 cagggaacca cagcccacca tgatgcggaa actccagaat accggcgcaa cctgaggtat
    11161 cgcgccacgc atcgccgcct ggtactgtgc ggctgtgaca tgattcatat ccggcgcata
    11221 gcgggagagc aacatgccgt agcccaggtc gccttccagc tcccggaatg cctgcaacac
    11281 atgcggctca cgattgcctt gcgatatttc ctgcattaac agccaggcca tacgtccgcg
    11341 ttgcaagcgt gggtaggttt cagccatcaa attcttcaga cccggcacgg gtttatctaa
    11401 tgagtgagtg gcgaggatcc ctagcagcgc gggaattttg atggcaaagg cgttacgctc
    11461 ttgatcctgt tccggccagg caaccacatg gaacggtgca ggtgcaggtt ccgtttgcca
    11521 ctccccttcc atcgccgcca gttttaccgg ttgtacttgc gcgacttcat acgcagaact
    11581 gtctccgagt tgcagggtac cgataatcgc cagagtaccg aagacggaac cgatggcaaa
    11641 cgagcgtaat gcgacatcgc gctcgcgtcc gcgcagtaaa taccaggcgc tgatcgccat
    11701 aataaacatg gccccggtca cgtagcccgc cattacggtg tgcacaaatt tcacctggct
    11761 gaccgggtta aagaccagct cgctgaagct ggtcatctcc atacgcaggg tgtcgatatc
    11821 aaaatgcgca ccggtcgggt attgcatcca accgttggca ttcaatatcc acaatgcaga
    11881 gagatttgaa ccgaacgcca ccagccacgt caccagcagg tgctggtatt tattcagccg
    11941 ctgccagccg aagaagaaca gcccgacaaa ggtggattcg aggaagaagg ccattaatgc
    12001 ttccatcgcc agcggtgcgc caaaaatatc gcccacatag ttggaataga atgaccagtt
    12061 agtaccaaac tgaaactcca tggtcaggcc ggtagccacg ccaagagcaa agttgatacc
    12121 gaagagctta ccccagaagc gcgtcatatc gcggtagatt gttttgccgg tgaccacgta
    12181 aatggtttcc atgatagcca gcaaaaaaat cagccccagg gtaaggggta caaataaaaa
    12241 gtgatacagc gcggtcagcg caaactgcca gcgcgataaa tcaatgacat cccacatgcg
    12301 cactcctgta ggcgaagaga gaaaaataac ccgttaatgg tagatctccc tctttatcct
    12361 gaaactctac gaccagaggt attcgccgat tgcgatattc ataagcctga aagcgatact
    12421 taccgtcttt ttacgtcggt gcagcttccg ccagccactg acatacctcg ctaagccgct
    12481 gcgcagagtc gaccaaatct tctggcgctg ccagcaccac ttccggtatt gggcagattt
    12541 cataactttc cagtaaaggg ccttttaagg tgtccgtaca gcgtaaatgc cagacatggc
    12601 gtaaccccgt ggcgttgata tagctctcgc catagccaat ggtacgaatc tgaatatttc
    12661 ccggcccaca gagacgtgag agaaaaagcc ggtcagcctc gctgatgggt aattgcgtca
    12721 gattaatgct gtgcggctgc gcgtcggggt tacgtacatg cgccagtaac tcatgcgcca
    12781 acggtaggcc attcatcagg ccatcaatgg gcggcggtaa cagcgaatct gtcggcaaga
    12841 gattttgcgt ggccgcctgc cacaacgcca gcggcgcgca gccggcctcc agtttgtcct
    12901 ccagcaactt ttcgccgcga cgtctgcgca cccgccataa tccgcagaag atcgcctcct
    12961 gtatttcact ttcactgtcg tcagcctgct gaatgcgcac tgacacttcc ccttccccga
    13021 gaagcgtatt gataaagtga cgatcgtcgg cattgagatt taacagatcg actgccagca
    13081 cctcgcccaa cggcggctgg cgttcgctta gtttatgttg taactcgttt aacagcgcaa
    13141 tcacctgcgg gctgtgagca cattgctcca gggctgccat actcggttca tcattcacct
    13201 gacaggtgat cggcagtgga ttcatgctga aactgtcatc gttcggttgc gttcctggcc
    13261 ccagcagatg gaaaaaagtt tcgctcatga ggcacgctcc tgttgtggtt cgacaagccc
    13321 acgcatcagg tttatcagtt ccgcccacgg gtgaatacca ttcagcacgc cgcgatagtt
    13381 tccgccggta aacactaatg tggcgggaaa gcgaaagacg ccaaagcgat ctccgatggc
    13441 ttcgctctgt tcaaggtcag caatcgccac ctgccatgtg tagtcgggaa actcgcgcag
    13501 taattcgcca atcattaccg gattatcgct gacctctggc gtgcgtttcg ggtcactgct
    13561 taataacacc acgccgtctg gcgcttgcgt aagccagtcg tcaagacgga attcactaac
    13621 cggcgtccag ccgcgcgcca gcattcgttg ccacaacgca tcaaatggcg tgtcgttgct
    13681 catccctgct cctccagtgt catcctgtac tggcgcaagc gaacgccttc gtaattttcc
    13741 atcgacagac agtcataatt gagacagcgc gattcattag ccggttgcgg cacaattccc
    13801 catgcagcca gctgcgccag cgccgcctgt tccgctgcgg gcagttgctc ccgtgccagt
    13861 tcgctcaggc tgccgccgta gtcgtcgagc attgcgggtt gcagaccgac gagggcaata
    13921 tgtgctggca gatgtccgcg gatatctgcc agcgccagca cttcggagaa actgttctgg
    13981 tgcaggctca ttttcttcgc gctgagataa gccggaatac gttctccggc ataggttcgc
    14041 agcgttccag gttccagccc gtagtcaatg gcatcgagaa tcaacagatg gctggcgctt
    14101 tcgacatacc ccagcaagtt cagtccctga gtaccgccat cgacaatctc cacatactcg
    14161 ggccagtggt aatgggcata caaccgttcc gccacccgca cgccgaaacc ttcatcggcc
    14221 cacagcaggt tgcccagccc cattaccacc acgcgttgct cgctcatgaa cgctccttgt
    14281 tacttatttt gccaaatttg tggctacggt agccgttgac catggtggag atcaccgtgt
    14341 cgtcggacat gatgtcttca cgcagcgcca tatagacatg accgatcaca aacgcgccaa
    14401 tcagccacat ccccagccga tgccagctgt gaatgtccat tgagttgcct cccgtccagt
    14461 agaaaaattc caccacataa cggaacggcg caaaaatagc gtactggctg tgttcgctgt
    14521 acagcgcaaa gccggtgatg atcataaaga ccgacatcag gaaatagccg aacatcgccg
    14581 cctgggcgat ggggttatgg cctatatcgg cactcggacg ttttgccaga aacagatacc
    14641 agcggatttc ataccacacg ccctgccacc agcttttacg ccataccggc acgataaaca
    14701 gctcgcggga gtatcgattg ccaacaaaag cccagtagat ccgcatcagc aagaccacgg
    14761 taaaaaccat cccggcgctg aagtgaatta acctgatgta gcccatatag aacagatacg
    14821 tcgcctcgcc gctgacggaa ggtagcggct tgccgataaa gtatccggtg accatcaaca
    14881 ccgccatgca taacaccgtc agccagtgcc agatgcggac tggcgcttca aagacatagt
    14941 ggctgacaac gttgtcgctt ttctgttgca tgatgattct ccttcgctgt taacgcacct
    15001 gcacggagat cagctcgcta ccgtcgtcgc ccagcacgtg tgttgaacag gcgaggcacg
    15061 gatcaaagct gtgcagagta cgcaggatct ccagcggttg ctcggggatc gccattttgg
    15121 tgttcatcag cgccgcttca taagcgccaa tctgcccttt gggatcgcgc gggctggcgt
    15181 tccaggtggt cggcaccacg cactggtaga gatcaatctt gccatcgcga atggcggccc
    15241 agtggcctaa cgctccgcgc ggcgcttcgg taaagccgac accacggcac tctgtcggcc
    15301 aggttgctgg ttcccacttc tcagtggagg cggtggcgag attgccgttt ttgagattgg
    15361 tcatcagctt gtcgaagaaa tattgcaact tgcctgcggc ccactgcgct tcgtgcgcgc
    15421 gacacaaaat gcggcctaac gttgactgga tgccggaaag cggcaggttc aacgccgaca
    15481 tcatgcgatc gaccgactca acggtcgcag catcgccttt gtgataagcg attaacgtgc
    15541 gtgccagcgg ccccacttcc atcgcgttac cgcgccagcg tggtgctttg atccacgagt
    15601 agcgttcctg ttcattcagc tgctgaatgt tggtatcgct gcctttgaca tcgccggggt
    15661 tgtaccacgg gtcggtaatg ccatcgaatg gatgacgccc gacctgatcg ttgggataac
    15721 gataccaggc gtgatcgaca aactcctgca cctgctgcgg atcaaccaaa tccactggca
    15781 gcacattgtt gaagtcgccg ttaatcaccg cgccgccagg catcagcaga cttttctcac
    15841 caaagtcgtt ggcaatatcc gggaatgcgc cgtagctgag gacacattta tcagaaagac
    15901 ccgtgccgat ttcgctccac ggtttgttga actggccgat ggctaaggcg tcggggatca
    15961 tcacgttgtt aatgaagtct gccgtgcggg tgataatcga ctgcaccagg ttcaggcgtt
    16021 ccatattgac cgccccgacc gcgccgcttt cgtcaatgtt aatggcgcaa ggcatcccgc
    16081 cgacaatcca gtttggatgc gggtttttac cgccaaagac cgcgtggatt ttgacaattt
    16141 cacgctggaa atcgagagct tcgagatagt gggcaaagcc catcaggtta gcttctggcg
    16201 gcagtttgta ctgcgggtgc ccccagtagc cattgcggaa gatccccaac tggccgcctt
    16261 caacgaattt cttcaggcga ttttgtacgt cgaagaaata gccaggggat gatttcggcc
    16321 aagaggagag actttgcgcc agttcggagg ttttccgcgg gtcggctttc agcgcatcta
    16381 acacatcgat ccagtccatc ccggcaagct gatagaagtg caccagatga tcgtgacacc
    16441 agagcgttgc cagcataatg ttgcggatga tattggcgtt gtccggcact ttaataccga
    16501 tggcatcttc gatggcgtaa accgaagcta gagcgtgtac gccagtacag acgccgcaga
    16561 tacgttcaac gaacgcccac gcatcgcgcg ggtcgcgccc ttgcaggatg atctccagcc
    16621 cgcgaaacat ggtgccgcag gagacagcat tggtgatcac attctgatcg ttaatattca
    16681 cttcgcagcg catgtggcct tcaatgcgcg taatcgggtc gaccaccagg cggcgtccgg
    16741 cattattgat ggtgtatccc tgagtttcgt actgagtgct catgcctgtt tatcctcatt
    16801 gcctggctga tgttcggttt ctgtaggttg ctggttatga cgtctgcgct ggtcaacggc
    16861 gctggcgact gcgtgcacac caacagccgc tgccaccacg ccaagcgcgg ttaaaccgac
    16921 ggtatcggcg gtggaatgag tacccatttg cggaatatcg accacgcggc tgtagaacga
    16981 accgcgatcc cagaaaccat tttccgcaca gcccaggcag ccgtgaccag actggattgg
    17041 gaaagaaacg ccatcattcc agcgtgtgga ggaacaggcg ttataggtgg taggcccttt
    17101 gcagcccatt ttgtacaggc aataaccttt gcgggcagcg tcatcatccc aactctggac
    17161 gaactctccg gcgtcgaagt gagcgcggcg atagcattta tcgtggatac gctgaccata
    17221 aaacatcagt ggacggccca ttctgtcgac atctggcaag cgatcaaaag tcaccatgta
    17281 agtaatgatg gcgctcatca catccgggat cggcgggcag ccaggtactt tgataatggg
    17341 tttgtcggtg atgactttgt cgataggcgt tgcctgtgtc ggattaggtc gcgcggcctg
    17401 cacgcagccc caggacgcac aggttcccca ggcgataatc gcgctggctc cggcagcggc
    17461 acgtttgagt ttctcaataa acggtcgacc gctgctgata cagaacatcc cctgctcgcc
    17521 cagcggcgga ttaccttcta ctgcgaggat atatttgcca ttgtattgcg tgatgatgtc
    17581 ttcaaagact tcttccgcct gggttccggc ggcagccatc aaagtatcgt cgtaatcgag
    17641 ggaaatcagg gaaaggatga cgtcctttgc cagcgggtga gcggagcgga taaaagattc
    17701 ggtacagcag gtgcattcca gaccgtggat ccataccact ggaatgcggg gtttgttctc
    17761 cagcgcccag gcaatctttg gtgccattcc cgcgcctaat cccagcgacg tggcagccag
    17821 actacaatat ttgagaaagc tgcgccgggt aacgccctga cgccgcatgg cctggtaaaa
    17881 tgtttcctcg ttattcatat cgcacgtctc tcctccttgc gacaccggca ggacaccggg
    17941 cgcacatggt cggcaagcga cgtccctgta gcaaggcaaa ccatcgcttc acgtgagatg
    18001 ctgaaaacga aagctcatcc ttctgcactg gcgcacgtcg ccagaaagta atgttaataa
    18061 agcgtagtga aacttttgca caaaacaata caaactgtgt ggatttatct tttagcgata
    18121 aaaatggacc tatttttctt tttgccgggc ggtgggatgt ttagccagtt gctaaacgag
    18181 caaaagagaa ggaattcatg gtgatgaaat gatcaatctg ctgttatttc acgctatcaa
    18241 tgcacttttg gctgtttttc aggcaaacaa acaaattagg ggtttacacg ccgcatcggg
    18301 atgtttatag tgcgcgtcat tccggaagtg tggccgagcg gttgaaggca ccggtcttga
    18361 aaaccggcga cccgaaaggg ttccagagtt cgaatctctg cgcttccgcc aaataatcaa
    18421 ggggttacca aatggtagcc cctttgtttt ttggctcttg gtataaactt ggtataccaa
    18481 ttggtatatt tttattcgga tgagattatc ggtcatgagc ggaacctcag gttcctcgtc
    18541 tgatgccgct cttgcaacac gttacgctgc cgagtatttt tgtaaaacat ggactgctcc
    18601 aggcctgaac caggcagaag gatacaaagc tatctcagat ctgtcacatc actattttcg
    18661 tgcagaaggc tctagccctc cccaatcaat ggcgacaggt ataaataagg ttcgttgtga
    18721 tgcaggaatg aaagagaaac atgtaaactc actggatata tttacacata gttatccaga
    18781 aatttatgct ggagtagcat taacacttgc aggagtatgc agtaaagata aacaggcgat
    18841 gtataatatt acacaagaaa ccgtccgtat ccttcacgaa actgaaacaa aagattgaac
    18901 attctcaata aagagctgcg ccaagcagca catattctgc attgcagtaa gatatgcatg
    18961 cccagccccc tgtaaaccta taaatatttc aaatcacatt gatcatctta tagccatatt
    19021 ttttgtgaca ccctgtttca gtgaacatga agacactcac cttgttcctg atattcatta
    19081 gcatcatcct gattatttaa cttctctatt cagggaagcc cgtctgatac aaagcttcta
    19141 tttctcgtta aatattctcc attctggcgc caagccctat ctatagctga ttgaaaacaa
    19201 ctctaaaaac gcaataatat caaa
//
LOCUS       DAZUGK010000083        19049 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZUGK010000083 DAZUGK010000000
VERSION     DAZUGK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.083"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            163..1383
                     /gene="ybdN"
                     /locus_tag="ACHY5W_002501"
     CDS             163..1383
                     /gene="ybdN"
                     /locus_tag="ACHY5W_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS4447384.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1356..1984
                     /gene="ybdM"
                     /locus_tag="ACHY5W_002502"
                     /pseudo
     CDS             1356..1984
                     /gene="ybdM"
                     /locus_tag="ACHY5W_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(1985..3145)
                     /gene="ybdL"
                     /locus_tag="ACHY5W_002503"
     CDS             complement(1985..3145)
                     /gene="ybdL"
                     /locus_tag="ACHY5W_002503"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFS4447385.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQQQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3254..4342
                     /gene="hcxA"
                     /locus_tag="ACHY5W_002504"
     CDS             3254..4342
                     /gene="hcxA"
                     /locus_tag="ACHY5W_002504"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFS4447386.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4352..4549)
                     /gene="ybdD"
                     /locus_tag="ACHY5W_002505"
     CDS             complement(4352..4549)
                     /gene="ybdD"
                     /locus_tag="ACHY5W_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS4447387.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4731..6836)
                     /gene="cstA"
                     /locus_tag="ACHY5W_002506"
     CDS             complement(4731..6836)
                     /gene="cstA"
                     /locus_tag="ACHY5W_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFS4447388.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILVMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRIDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            7148..7570
                     /locus_tag="ACHY5W_002507"
     CDS             7148..7570
                     /locus_tag="ACHY5W_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447389.1"
                     /translation="MSYDKAKYHFDSVIEEGLPLSQAYIHTGFYLGWLIDNNLLDEEF
                     KEDFAESLALFTRREITAPQLFEEFDGTLTDEELSTQGNAFTKHYFDFDTGDYLEDYC
                     THLVKDLPSEFHVKDTWENYKIAASFISRRYQEWLHNQ"
     gene            complement(7681..8094)
                     /gene="entH"
                     /locus_tag="ACHY5W_002508"
     CDS             complement(7681..8094)
                     /gene="entH"
                     /locus_tag="ACHY5W_002508"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFS4447390.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVIG"
     gene            complement(8097..8843)
                     /gene="entA"
                     /locus_tag="ACHY5W_002509"
     CDS             complement(8097..8843)
                     /gene="entA"
                     /locus_tag="ACHY5W_002509"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFS4447391.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8843..9700)
                     /gene="entB"
                     /locus_tag="ACHY5W_002510"
     CDS             complement(8843..9700)
                     /gene="entB"
                     /locus_tag="ACHY5W_002510"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFS4447392.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMAQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9714..11324)
                     /gene="entE"
                     /locus_tag="ACHY5W_002511"
     CDS             complement(9714..11324)
                     /gene="entE"
                     /locus_tag="ACHY5W_002511"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFS4447393.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTSGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11334..12509)
                     /gene="entC"
                     /locus_tag="ACHY5W_002512"
     CDS             complement(11334..12509)
                     /gene="entC"
                     /locus_tag="ACHY5W_002512"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFS4447394.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQLLNVVERQAIPQQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12698..13654
                     /gene="fepB"
                     /locus_tag="ACHY5W_002513"
     CDS             12698..13654
                     /gene="fepB"
                     /locus_tag="ACHY5W_002513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4447395.1"
                     /translation="MRLAPLYRNALLLTGLLLSGISAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13658..14908)
                     /gene="entS"
                     /locus_tag="ACHY5W_002514"
     CDS             complement(13658..14908)
                     /gene="entS"
                     /locus_tag="ACHY5W_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFS4447396.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15019..16023
                     /gene="fepD"
                     /locus_tag="ACHY5W_002515"
     CDS             15019..16023
                     /gene="fepD"
                     /locus_tag="ACHY5W_002515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFS4447397.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16020..17012
                     /gene="fepG"
                     /locus_tag="ACHY5W_002516"
     CDS             16020..17012
                     /gene="fepG"
                     /locus_tag="ACHY5W_002516"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFS4447398.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGVRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17009..17824
                     /gene="fepC"
                     /locus_tag="ACHY5W_002517"
     CDS             17009..17824
                     /gene="fepC"
                     /locus_tag="ACHY5W_002517"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS4447399.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSITNS"
     gene            complement(17821..18954)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY5W_002518"
     CDS             complement(17821..18954)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY5W_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFS4447400.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFVKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRHAMASRKKDAMMADHLV"
BASE COUNT         4556 a         5360 c         4864 g         4269 t
ORIGIN      
        1 aaatgagtgt ttttgatata taacctcaga cagctactat aactttcacc tatttattca
       61 cagcgcgaat gaaaaataaa acactctgct tttcagtgtt tactttataa ttaacagtct
      121 tatctcactg atcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt
      181 cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac actcaacacc
      241 ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg
      301 acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggag
      361 gcgcaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc
      421 atcgaagagt tttactgggt tgcgctcccg cttacgacgc aaaattccct ttcacaatac
      481 caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat
      541 gcgataaccg atcctgactt tttctgcttt taccagccag gcatgacctt cgaacaattt
      601 gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc
      661 cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt
      721 gccgacgata aaccctggac tacggccgca ccaggcggtc atagctggta catttatccc
      781 atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca aagtctctgc
      841 aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc
      901 gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat cgaaccggat
      961 cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga
     1021 catgacaatc atttctatgg gcatcggaaa atcctcaagc cagaacattt agactggcaa
     1081 gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat
     1141 aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag
     1201 caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc
     1261 aataacgatt actggtgtcg agcattatca tttagcccta ctaaagcgaa gaactatcag
     1321 cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat
     1381 taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaacgcga
     1441 ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt
     1501 gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg
     1561 caccacccga aaaaaactgc tgaaaaaatc cattgaaata gatggtttta cccaacccat
     1621 tgtggtcacg catactgata aaaatgccct ggaaatcgtg gatggttttc accgccatga
     1681 aattggtaaa ggctcatcat cattaaaatt acgcttaaaa ggttatttgc cggtgacctg
     1741 tctggaaggt acgcgtaatc aacgcatcgc cgcgactatt cgtcataatc gcgctcgtgg
     1801 acgccatcaa atcaccgcca tgtcggaaat cgtccgtgaa ctcagccagt tgggatggga
     1861 cgataataaa attggcaaag agctgggaat ggacagcgac gaagtgctgc gcctgaagca
     1921 aattaacggt ctgcaagagc tgtttgccga ccgtcaatat tcccgcgcct ggactgtcaa
     1981 ataactacag ctggcgcagg cgttcagctg ctgccagcaa cgtcgattcc ttcttggcaa
     2041 aacagagacg aatcagttta tgagggaagg gatcggcgca aaacaccgat agcggaatcg
     2101 ccgctacgcc gtgctcctgc gtcagccact ggcaaaactc aacatcatcc agggtagata
     2161 cggcgctgta atccaccagc aaaaagtatg taccttcaca cggtaaaatc tccagccggc
     2221 tttcatttaa agcattcacc agaatatcgc gcttctggcg ataaaagtcc ggtaacgcaa
     2281 gataatgctc aggttctgca cgtagcatat cagcaagcgc cagctgtgcc ggggtattca
     2341 ccgaaaaggt cagatactga tgtaccttgc gaatttcggc gctgatgggc gctggcgcaa
     2401 cacaataacc cactttccag ccggtcatat gataggtctt gccaaatgaa gaaaccgcca
     2461 ctgcccgctc acgcagctgc ggatgcgcca gcacactggc atggccctgt tgtgaaaagt
     2521 tgatgtgctc gtagacttca tcgctaatga caaaaatctc gtgcccggcg atcgcctgcc
     2581 acaaagcggc gaaatcagcc tgctgccaga cagttgcact ggggttatgc ggagtgttga
     2641 ggatcaccag tctggtgcgc tcgcttaaca atgcggcaaa ttcctgccag tcaacgcgaa
     2701 aatgcggtgg ttgcagtgcc atacgcttca ctattccccc agaaagcgcg atggcggggg
     2761 cgtaactgtc atagctggga tcaaaacaaa tcacttcgtc gccattgcgc accaatgcgg
     2821 ttatcgccgc gtataacgct tccgtcgccc ctgccgttac ggtaatatcg ctatcggcat
     2881 ctggttgata gccatacaaa cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca
     2941 cgccggtcat gggcgcgtat tggtttgccc cctgggcaac gtggtacgcc agccgctcct
     3001 gtaaatagcg cggaccatca aaatcaggaa agccttgcga caggttaatc gcctgctgtt
     3061 gctgcgccag cgcgctcatc tgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt
     3121 gtggaatcag agggttattt gtcattttta ttgtacctga cgaagttgcg tgttgaaggc
     3181 actataacac gatgctagta tttggcaatc aagacgttta gatgtctaaa taaaacaata
     3241 aggacaacac aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg ctaactactt
     3301 ttcacatcca ggaagtttca atcacctgca cgattttttc actgatgaac aactttctcg
     3361 cgcggtgtgg atctacggcg aacgcgccat tgctgcggcg caaaccaaac ttccgccagc
     3421 gtttggactt ccaggggcaa agcatatttt gtttcgtggt cattgcagcg aaagcgatgt
     3481 acagcaactg gcggctgagt ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg
     3541 cgcactactc gacaccgcga aagccctcgc ccgccgtctc ggtctgccat ttgttgccgc
     3601 tccaacgatc gccgccacct gcgccgcctg gacaccgctc tccgtctggt ataacgatgc
     3661 cggacaggcg ctgcattatg agattttcga cgacgccaat tttatggtgc tggtggaacc
     3721 agagattatc ctcaatgcgc cgcaacaata tctgctggcg gggatcggtg atacgctggc
     3781 gaaatggtat gaagcggtgg tgctggctcc gcaaccagaa acgttgccgc taaccgtgcg
     3841 actggggatc aataatgcgc aggctattcg cgacgtcttg ttaaacagta gcgaacaggc
     3901 gctgagcgat cagcaaaatc aacagttaac gcaatcattt tgcgatgtgg tggatgctat
     3961 tattgctggt ggtgggatgg tcggtggtct gggcgatcgt tttacgcgtg tggcggcggc
     4021 tcatgccgtg cataacggtc tgaccgtgct gccgcaaacc gagaagtttc ttcacggcac
     4081 caaagtcgcc tacggaattc tggtgcaaag cgccttgctg ggtcaggatg atgttctggc
     4141 gcaattaact ggagcgtatc agcgttttca tctgccgact acgctggcgg agctggaagt
     4201 ggatatcaat aatcaggcgg agatcgacaa agtgattgcc cacaccttgc gcccggtgga
     4261 gtccattcat tacctgccag tcacgctgac accagatacg ttgcgtgcag cgttcgaaaa
     4321 agtggaatcg tttaaagcct gacgtgcaaa tttagcagca gcgcgcgccg ccttttccac
     4381 cgtagcgcgc gtcctgccgc tcacggaaaa actcttcgta ggtcatcggc gtttgatcgg
     4441 gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca atcatcagtt
     4501 tcgccgcctg acctaaatat tttccggctt ttgccagtga atcaaacatc ttggcctccg
     4561 ctaaccatgt tgctgaaaga gtgccggatg cggcgtgaac gccttatccg gcctacaaat
     4621 gctatcaacg cgctcggtca gttccctcac cccatcgggg agagggttcg ggtgaggggg
     4681 aacccacggc gccatgccaa ctttcgccaa catcccccct cactctgact ttagtgcgcg
     4741 ccttttgcct gcgccacgat ctcctcaaca ttttccggca tcggctcata cggcgtttct
     4801 ttcgccgttg gcttcggatc tttcaatgcc gcaagtgccg tcttaatcga gaacagtgcc
     4861 agaaccacca cgaccaccat aaagaagatg gttaacccgg catccagacg gttgttgaac
     4921 accagttgcg ccagctgtga ctcagtatac tgcgacggaa tattgccgct gtcgatcatt
     4981 gcctggaact tgttagcaat ggccaggaag ccgactttcg catccgggct aaacgctttc
     5041 tgccagccag cggtcagggt acaaatcagc agccaggccg ttggtaccag cgccacccag
     5101 gcgtaacgtt gacgtttcat tttgaacagc accacggcac agagcatcag cgccatccct
     5161 gccagcatct ggttggcaat accaaacagc ggccacagag tgttaatacc gcccagcgga
     5221 tcgaccacac cctgatggag gaagtagccc cacgccagta cgcacagcgc cgttgccagc
     5281 aggttagcag gcagtgaatc gatccgcttc aggccaggag acaccacgcc cagcagatcc
     5341 tgcaacataa agcgcgcagc acgcgtaccc gcatctaccg ccgtcagaat aaacagagct
     5401 tcaaacagaa tggcgaagtg ataccagaac gccacatcca tcataccacc cagcgcaccg
     5461 tgcaggatgt aagccatccc caccgccaac gtcggcgcac cgcccgcacg ggaaatgatc
     5521 gactgttcac ccacttcgct ggcaatctgg tttagcgtat ccggggtaat cgcaaagccc
     5581 cagctactca ccacttgcgc cgcagaagcg accacatccg ccgtccctgc cggagccagc
     5641 accgccatcg ggctgttcat ggcgaaatag acgcccggat cgatgataca ggcggaaacc
     5701 agcgccataa tcgccacgaa ggattccatt aacatcccac cgtagccgat aaaacacgcc
     5761 tgcccttcgt tcgccagcat cttcggcgtg gtgccggaag agatcagcgc atggaagcca
     5821 gacaccgcgc cacaggcaat ggtgataaac aggaacggga acaggttacc ggtccatacc
     5881 gggccagtgc catcaacaaa tttggtcaac gcaggcatgg tcagcgtcgg acgcataacc
     5941 aaaataccca ccgccagacc aacgatagtc ccgattttca ggaaggtaga gaggtagtca
     6001 cgcggggcca gcagcagcca caccggcagc accgccgcca caaaaccgta acccaccagc
     6061 atccaggtca gctgcacgcc ggtaaagtca aagtacggtg cccaggtcgg actttctgcc
     6121 acccagccgc cagagataat ggcgaaaatc aggaatacca gaccgatgac cgacacttca
     6181 ccaatacgcc ccggacgcag atagcgcagg tagatcccca taaacagcgc cagcggaatg
     6241 gtgaacgcaa cggtatacgt tccccacggg ctatgagtca gggctttcac cacgatcatc
     6301 gccagcacag caaggataat gaccatgatc ataaagcagg ccaccagcgc gatcaccccg
     6361 gcggttggcc ccatctcttc tttgaccagc tcacccagcg aacgaccgtc acggcgcgta
     6421 gaaacaaaca gcaccatgaa atcctgcacc gcaccggcga gaaccacccc ggcgagtagc
     6481 cagatcatcc ctggcaggta acccatttgc gccgccagta ccggcccaac caacggacct
     6541 gctccggcaa tggccgcaaa atggtgaccg aacagcactt tcttgtccgt cggcacatag
     6601 tccagcccgt cgttatgccg caccgctggc gtcatacgcg tcgggtcaac cgccagcaca
     6661 tttttggcga tatacagacc ataaaaacgg taagcgatca gatagataca gaccgacgcc
     6721 accacaatcc acagcgcgtt gatctgctcc ccacgattta aagcaatgta tcccagagca
     6781 aatgctccca ttacagagag cactgtccag acgaggtatt tccctgactt gttcatagtt
     6841 gttatccgtg tgcgtgtcca tagagatgtt acattttgtt tctatatcaa cagattttat
     6901 ttaacaactc agaaaccaac atacacaaca aaaacaccac atatttacat cacattgtta
     6961 acgagatcac tttagtcgcg aaaagacgtg tacaactacg gacaattgag agggtcgact
     7021 caccagatta gtaacactac ttataggtga aaatgcatag acgatatcta tagtattctg
     7081 cgtcaatgtt atgcaacgca accatacgat agcatggcta tttcattata tgataaggag
     7141 tccctccatg agctacgaca aggcgaaata ccattttgac tcggttatag aagaaggttt
     7201 accgctcagc caggcataca ttcatactgg tttttattta ggttggttga ttgataataa
     7261 cctgttggac gaggagttta aggaagattt cgctgaatct cttgccctct ttacgcgcag
     7321 agaaataaca gcccctcagc tttttgaaga attcgatggt actttgaccg atgaagaact
     7381 ttcgactcaa ggaaacgcct ttactaaaca ttattttgat tttgatacag gcgattatct
     7441 cgaagactac tgcacccatc ttgtcaaaga tcttcccagc gaattccatg taaaagatac
     7501 gtgggaaaac tataaaatcg cagcaagttt catatcaaga cgctatcagg aatggctaca
     7561 taaccaatag tctcaccctc tgcacatatt tttccgggta gcgctttcac ttacctggcc
     7621 aacaaattct cgcgaatcgt aggcctgata agcgaagcgc atcaggcata ttaatccgat
     7681 ttagccgatc actgccgtac ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa
     7741 aacgacgatt tcccagctct gattttgccg cccaagatgc agcggctggc agacgccgcg
     7801 taccttgcct tcagacaccg ggcgatgatg ggtggcgtta agttccgtgc ccaccacaca
     7861 ctgcccgtca cgggtcatca taaatccggc catcgatccc agcgtttcag ccagcgccgc
     7921 cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta cgggtatcaa ccggcatttc
     7981 ggcttccagc acatcatcgc ccagacgggt atacacaatt cccagatgcg ccaccattgt
     8041 gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga tcatgcttat
     8101 gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtgatatg gctggcgagg
     8161 tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat tttccccagc
     8221 ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc ggcgtcatcg
     8281 ctcacccaca gcgtgcgttg catatcggta tcggtggaac ccggcgaaac cacattacag
     8341 cgcacgccgc tacccgccag ttccagcccg acgctcaacg ccaggctttt cagcgccgct
     8401 ttcgatgcgc cataagcgct cataccaata cgcggcgtgt gtgcggcgtc ggacgccaca
     8461 gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg gaacaggtta
     8521 aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt cctccttact gagctgatcg
     8581 gtcgcgccca tgcgcaaaat tcccgccgca ttgaccagca cgtccagtcg ctccgtttca
     8641 gctaacagtc gctgacatac ctgcgccacc tgcccggcgt cggcaacatc cattacttcg
     8701 gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa aaccggtaac tttcgctccc
     8761 gcttcaacaa acgccagcgc cgtggcgtag ccaatacctt tccccgcgcc agttacccag
     8821 acatttttgc cgctaaaatc catcatttca cctcgcggga gagtagcttc caccaggcgt
     8881 cgatggtcgg gtttttcgcc agcatgacaa aatcgatatc gccatgcact ttgcgccagc
     8941 gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat
     9001 cgaacggttc atcggactcg tccagcaacg gcaggataac ctcacgcagc gccgctttgc
     9061 tggcggggat aggtgctggc agtaattctt cggtcatcac cacccggcca gaacgtccgg
     9121 ccacatattt cagcgacatt aaatgctcgt cacggctgaa atcggccagc gcatccgcca
     9181 ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata cagccaatgt
     9241 gggcatatac ccctgtaata atcagctggt tacgtccact ctctttgagc atttgctcca
     9301 gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg
     9361 gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg ggtcaggccc ggcccccaca
     9421 tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa
     9481 ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg atcacctgcg
     9541 ccatcatcgg gcagttctca ccccagaagc tgacaaaata gtcctgcata tcatggatta
     9601 acaacgcggc acgttgcggt tcaaaggccc agtcgacttt attcttaggg atatcgtgag
     9661 actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct ccttcaggct
     9721 gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc gaccgccgtc
     9781 agcggaagtg aatccacaca ctccacgcga tccggtaatt taaattcggc aataccctgt
     9841 tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct cttttaccac cagataagca
     9901 cagctttttt cgcccatcag ctcatcttcc atactgacaa gtgcggcgta gatcaccgcc
     9961 ggatggcgca gtaacaggtt ttcgatctct tcggcagcga tcttctcgcc gccacggttg
    10021 atctgatctt tctcgcgccc ctgcacggtg atgtaaccct ctggatcaat agagatcaga
    10081 tcgccggaac agtaaaaacc gttggcatca aaggcgctgg cattgtgctg cgggcttttg
    10141 taatagccac ggaaggtgta cggcccgcgc gtcatcagtc gtccgacttc cccttgcggc
    10201 agtggatttc cttcggcatc ggcaacccat acttcgtcat ccggacacat tgggtaaccc
    10261 tgggtatgga taattttctc cgcgctatca tcaagacggg tatagttcac cagcccttcc
    10321 gccataccaa acacctgctg caactggcaa ccaatctcag cgggaatacg cgccgcaagc
    10381 gtggcagaaa gacgtgcgcc accgacctgt aacagtttca gcgaggcaag ctgcgcccgg
    10441 ctttcgcctt cggtcagcgc ctgcaaccac aggctgactg ccggcggcac cagcgcggta
    10501 acgttaatct gatgtttttc aatcaatggg aagcaaagcg tggcgctggg atcggccgcc
    10561 agaacaaccg ttcctccggc aagaaagacg cccagcgatc ccggcgaact catggcgtag
    10621 ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg acaaatctcg
    10681 acgctacgac gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag cttcggtgta
    10741 ccggaggtgc cgccggaaag ctggaaatag gccacttcat cagcaggtga cggcgtagca
    10801 gtaaaaccgt ctgccggatg gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg
    10861 agtagctgca ccacgcgaat ggaagaatgc tctgcaacaa atgtgttgag gaaatcatcc
    10921 ccgctaaaca gcgcatgttg gcgatcggca atcagcaatg cgggttcaat ctggctggca
    10981 taggcgttca gttcactacg ctgatggctg aacaacgcca gcaccggcgc aacgcccagt
    11041 ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt tacccagttg taccagcgcg
    11101 gtttcaccag gtttaatgcc ctgacggcgt aaactacacg cgaggttatc cgccgcctga
    11161 ttcagctccc ggtaactcaa ctgtcgctcg ccgtcgataa ccgcgatgct gtcactcgca
    11221 gcgtggcgag tcagaatgtc agtcagtggc aaatcctgcc agtagccttt ttcccgatag
    11281 cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc
    11341 aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac
    11401 ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt ttcccgcagc
    11461 ttcgcgcaac ggatggtcac cacccattcg ccgttacctt cgctgtcaca ccaacccaca
    11521 atgccgccaa acagttcgcg gtcgaacggc tccagttcag caataacctg ggtcgcggcc
    11581 tgatgcggga aaccgctcag cgcgggggtc ggatgcagca gacaggccag agtcagtgcg
    11641 ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag cgtcggcgtg
    11701 gtaatcaatt gtggagagga gggaacgtgt aactcactac tgcgttcgcg cagtacctct
    11761 ttcatcgcct gagtcaccag ttcatgttca tggcgatctt tttctgacgc cagcagacga
    11821 ttacccgctt cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc ggctaacgga
    11881 atggagctaa aacgctcgcc gtctttacgt agcagcagtt ccgggctggc ccccagcaga
    11941 acgccaccat ctgccaacgg cacatggaag ttgtaactta ccgggttttg cgcaatcaac
    12001 cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat caaccgtgac
    12061 aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cagcgcgagc aaccatctgt
    12121 tcaaacgtgg tttgttgcgg aattgcctgg cgttccacca cattcagcaa ctggctgcgg
    12181 gtgaaacggc gtgcggaagt ttgtttttcc tgacgggaga acgactgcca ggattcggga
    12241 atatacagcg acgaaggctg acgtggatcg aagggaatcg ccccgaccat caccggattt
    12301 ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg gaagggactg
    12361 tcgggcgaat ccccattcac agccggttca tcgaagcggg cgaaacatcc tgacgtcgta
    12421 aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc catggtctgc
    12481 tgtacttcct cagccagtga cgtatccata tcatcctcca caaaatgata aaggctttaa
    12541 taatgattat catttatatt ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac
    12601 tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttac aatgaaaatg
    12661 agaagcatta acttcattaa taacaggaag ctgatttgtg agactcgccc cgctctaccg
    12721 caacgccctt ctattaacag gacttttgct ttcaggaata tccgcagttc aggccgccga
    12781 ctggccgcgt cagattactg acagccgtgg cactcatacc ctggaaagcc agccgcagcg
    12841 tattgtttcc accagcgtca ccctgaccgg atcactgctg gcgattgatg ctccggtgat
    12901 cgccagcggc gcgaccacgc cgaataaccg cgtcgcggat gaccagggct ttttacgcca
    12961 gtggagcaag gtggcgaaag aacgcaaact gcaacggctc tatatcggcg aaccgagcgc
    13021 cgaagccgtt gccgcgcaaa tgccggatct gattttaatt agcgcaaccg gcggtgattc
    13081 ggcgctggcg ctgtacgatc agctttccac catcgcgccg acattaatca tcaattacga
    13141 cgacaaaagc tggcagtcgc tgttaacgca gcttggcgaa attaccgggc atgagaaaca
    13201 agcggcagag cggattgcac agtttgataa gcaactggcg gcggcgaaag agcaaatcaa
    13261 attaccgccg cagccggtca ctgccattgt ctataccgcc gctgcacaca gtgccaatct
    13321 ctggacgccg gaatcagcac aagggcagat gctggaacaa ctcggcttta cgctggcgaa
    13381 gttgcccgca ggcttaaacg ccagccaaag ccagggcaaa cgccatgaca tcattcagct
    13441 tggtggggaa aatctggctg cagggttaaa tggcgagtca ctattcctgt tcgccggtga
    13501 tcagaaagat gccgatgcta tttatgctaa tccgctgctc gcgcacctgc ctgcagtaca
    13561 aaacaagcag gtttatgcgc tgggaaccga gacgttccgt ctggattact acagcgccat
    13621 gcaagtgctg gaacgactta aggcgctgtt ttaagcatta accgtcagac gctgtcacct
    13681 gcggcggcgt ctggcgaaaa cgtcgcaact ccaccagcac cagcaataac aacacgccga
    13741 taatcaataa accaaaaccg ctcgcgcttg cggaagcaac cggtgtcatc atcgctccca
    13801 aaccacccag cagcgccgcg cctatggcat cgcccgtcac gttttgtgcc gtccacaaac
    13861 cgttaatccg ccctaacatc gcttccgggg tttgcgtttg cagcattgtg tattgcagca
    13921 acgagctgac cgcactcaac cagccgaaca gcgccagaca aaccacgcct aaaatccaca
    13981 tcggcatcag gccaaacaga ccaatggcga ggaacgatcc cagcgtggag agcagcatca
    14041 ataaccctgg tcgcgcgcta tgcgccagct tcccgctggt taacgcgcca atagccgcgc
    14101 cgagcgggat cgccgcatag agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag
    14161 ccagcgccgg atacagtacc cgcaccgcac tcgccatcgt caataaacca cccagcagcg
    14221 caatccctcc caccagcggg ctggcgagca gaaaacgaaa tcctgccagt aatgatttca
    14281 acggatgctc acgcggctgc ggtggcggtg gcagtgccgg aaggcttaac aacggtagca
    14341 aggtaataaa cgtgcccgcc gccgccagcc cgtagttcca ggctacgcca ccggtcgcca
    14401 gcaataaacc gccaatcatg ggcgaaatca ccgaccccag acgcacggtc aacatggtga
    14461 tcgccccggc ctgcattaag ttttcacgcc ctaccagtgc aggtgtcgcc gccagtagcg
    14521 ccgtaacgcc aagcgatgcg aaaaaaccat cccataaacc aagtaaatag attgccagca
    14581 atgacggctc cggcagcagc gcgttcaggc acagtccaat gaagccaatg ccacaggtgc
    14641 cgcgcgccag caaaatcact tttttgcgtt catagcgatc cgccagcaca ccgccgacca
    14701 tcaggccaac aaacatcgcg ccgccggtca gcgtcaccga aagccccacc tgccaggtag
    14761 aatgcgtcat catctggatc tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa
    14821 ttgagataaa acgagcgagg aatactgcgc gaaacgccgg gtgcgttttc aacaggctga
    14881 ggttaagcag ccaggattgt ttattcatta caatgccttg ccatcaaata ttttaaatac
    14941 ctattcttag gctgcacatg ctaacatatc caaataagat cgataacgat aattaatttc
    15001 attatcatgg aagttcgtat gtctggttct gttgccgtga cacgcgccat tgccgtgccc
    15061 ggattgctgt tattactgat tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca
    15121 ctccccgctt ccgtagtgct ggaggccttc tccggcacct gccagagcgc cgactgcacc
    15181 atcgtgctcg acgcgcggct gccgcgtacc cttgccggtt tactggcagg cggcgcgctt
    15241 ggccttgccg gggcgttgat gcaaaccctc acccgaaatc cacttgccga ccccggcttg
    15301 cttggcgtga acgccggagc cagctttgcc attgtgctgg gcgcggcgct gtttggttac
    15361 tcatccgcgc aggaacaact ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt
    15421 gttgccttta ccggcagcca gggcggcggg cagttaagtc cggtgcgttt aaccctggcg
    15481 ggcgtagcgc tggcggcggt gcttgaaggg ctgaccagcg gcatcgccct gctcaaccct
    15541 gacgtctacg atcagttgcg tttctggcaa gccggttcgc tggatattcg taatctgcac
    15601 accttaaaag tggtgctgat cccagtgctg atcgccggag caaccgcgct attactgagc
    15661 cgcgcgctga atagtctgag tctcggcagc gacaccgcga cggcgctggg cagtcgcgtg
    15721 gcacggacgc agttaattgg cctgctggcg attaccgtgc tttgtggtag tgcgacggcg
    15781 gtagttggcc cgattgcctt tattggcctg atgatgccgc atatggcgcg ttggctggtg
    15841 ggtgccgatc atcgctggtc gctgcccgtc acgctacttg ctacccctgc cctgctgctg
    15901 tttgccgata tcatcgggcg ggtgattgtg cccggcgagc tgcgcgtttc tgtggtcagt
    15961 gcgtttattg gtgcaccggt gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca
    16021 tgatttacgt ctctcgccga ttactcatca cctgtttgct gctgatttcc gcctgtgtgg
    16081 ttgcaggtat ctggggagta cgcagcggtg ccgtcacgct ggaaacctcg caggtattcg
    16141 ccgcgctgat gggcgatgcg ccgcgcagta tgacgatggt agtcaccgaa tggcgtttac
    16201 cacgcgtgct gatggcgctg ttgattggcg cggcactggg cgtcagcggc gcaatttttc
    16261 agtcgctgat gcgtaacccg ctcggtagcc ctgacgtaat gggctttaac accggggcgt
    16321 ggagcggcgt gctggtggcg atggtgctgt ttggtcagga cctgacggct atcgcgctgt
    16381 cagcaatggt gggcggcatt gtcacttcgc tgctggtctg gctgctcgcc tggcgcaacg
    16441 gcatcgacac ctttcggttg attattatcg gtatcggcgt tcgcgccatg ctggtggcct
    16501 ttaatacctg gctgttgctg aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga
    16561 atgccggatc gctcaatggc ctgacgtggg caaaaacttc gccttccgca cccatcatta
    16621 tattgatgct aattgccgcc gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg
    16681 atgataccgc ctgcgcgctg ggcgtcagcg tcgaacgttc gcgtctgtta atgatgctgg
    16741 ttgcagtggt gcttaccgct gcggcaacgg cgcttgccgg gccgatttcc tttattgctt
    16801 tagtcgcgcc gcacattgcc cgacgcatta gcggcaccgc tcgctggggg ctgacccagg
    16861 cggcgctgtg cggagcgctg ttactgctgg cggccgatct ctgcgcccaa caactgttta
    16921 tgccgtatca acttccggtt ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg
    16981 tcttgttaat tcaggagtct cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga
    17041 acagttaacc ctgggatatg gcaaatatac cgttgcggaa aatctgactg tagaaatacc
    17101 tgatggtcac ttcacggcaa ttatcgggcc aaatggctgc ggtaaatcca cgttactgcg
    17161 taccttaagc cgcctgatga cgcctgctca tgggcatgtc tggctggatg gcgagcacat
    17221 tcaacattac gccagtaaag aagttgcccg gcggattggt ctgttggcgc aaaatgccac
    17281 cacgccggga gatatcaccg tgcaggagct ggtggcgcgt gggcgttatc cgcatcaacc
    17341 gctgtttacc cgctggcgca aagaggatga agaagcggta acgaaagcga tgcaggccac
    17401 gggaataact catctggcag atcaaagcgt ggataccctt tccggtgggc aacgccagcg
    17461 agcgtggatc gcgatggtgc tggcccagga aacggcaatt atgctgctcg acgaacccac
    17521 gacctggctg gatatcagtc atcagattga tttgctggaa ctgttaagcg aactaaaccg
    17581 cgagaaaggc tataccctgg cggcggtgct gcacgatctt aatcaggcct gtcgttacgc
    17641 cagccatttg attgcattgc gggaagggaa aattgttgct cagggagcgc cgaaggagat
    17701 tgtcactgct gaactgattg agcgcattta tggtctgcgc tgcatgatca ttgacgatcc
    17761 agttgccgga acgccgctcg tggtgccgct cggacgaacg gcaccttcaa tcacaaatag
    17821 ttaaactaag tggtcagcca tcatggcatc cttttttctg gatgccatcg catggcgcaa
    17881 taacacgcta ccacaagcca ccatcccgcc gatcagcgcg gaaaggatca caataatcgc
    17941 cttacccgga ccgtcttttt tcactggcaa tgacgggctt aactgatatt taaacggcgt
    18001 aaaattcaca tcgttgatat ttgcttttgt taactgctca acaagatact gacgattacg
    18061 taattcaccg ttcagttccg caacgtcagt gaccgctttt tcaatttcca gtttgcgttc
    18121 aataccgtct gcgccgagag aaatagagaa atcggggtca tctttaacgg cctgaccatt
    18181 actgtaaacg ggctttttaa ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg
    18241 ctgaatgttt gcatcaagtt gatttttcat tttaatgcgg tcctgagcca gtttttcttt
    18301 ttcaaactga gttttgatct ccagtttatt acggacgttt tctatcgact ctttcaccac
    18361 caacgcagag atataatcga tatacccgct caaaacggtc tgcgcctctt cactggttgg
    18421 cgcggtaaaa cttagcgtcc aggaggtata cagtgacggt tcatcttttt tcttactggc
    18481 attgtcatca accgctttca ttttttcgct caatgcgaca attgcgcgat gcaaatccag
    18541 ttcgtcgatt ttcgcctctt ttaattggtc catcacataa ggtgatgaac gcaggtactc
    18601 ttccagcaag ctaaccgact gaaacttctt gacaaacagg ttaaatgctt ctgtacgatc
    18661 aattttgata tccagatcca gcacacgaag cttagtgaat gttttctcca actcttgcca
    18721 ctgaacaggt tctggaggcg tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa
    18781 agagatcagc aagcctgcgc aggcaaacgc aaaaacgacc gccatgaccg tttttttggc
    18841 ccgccataaa actgagatta gattaagtaa atcaatttca ttattactgg gcgacgccag
    18901 aggataatcg ggaaaatgag cgtcacttcc ctgtttaata ttcagtgatg acatggggta
    18961 taacctgaaa atgagtccaa tagggggaaa tctccacggc gctcacttta ccagttggtg
    19021 agaattatcc gaagctgaag tttgtaaat
//
LOCUS       DAZUGK010000084        18952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZUGK010000084 DAZUGK010000000
VERSION     DAZUGK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.084"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..962
                     /gene="ybhD"
                     /locus_tag="ACHY5W_002519"
     CDS             9..962
                     /gene="ybhD"
                     /locus_tag="ACHY5W_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4447401.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESELCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1003..1998)
                     /gene="pgl"
                     /locus_tag="ACHY5W_002520"
     CDS             complement(1003..1998)
                     /gene="pgl"
                     /locus_tag="ACHY5W_002520"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFS4447402.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2153..2971
                     /gene="ybhA"
                     /locus_tag="ACHY5W_002521"
     CDS             2153..2971
                     /gene="ybhA"
                     /locus_tag="ACHY5W_002521"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFS4447403.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALTRAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2972..4030)
                     /gene="modC"
                     /locus_tag="ACHY5W_002522"
     CDS             complement(2972..4030)
                     /gene="modC"
                     /locus_tag="ACHY5W_002522"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFS4447404.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4033..4722)
                     /gene="modB"
                     /locus_tag="ACHY5W_002523"
     CDS             complement(4033..4722)
                     /gene="modB"
                     /locus_tag="ACHY5W_002523"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFS4447405.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     SLLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLVSEW
                     LARISRERAGR"
     gene            complement(4722..5495)
                     /gene="modA"
                     /locus_tag="ACHY5W_002524"
     CDS             complement(4722..5495)
                     /gene="modA"
                     /locus_tag="ACHY5W_002524"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4447406.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVIAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5662..5811)
                     /gene="acrZ"
                     /locus_tag="ACHY5W_002525"
     CDS             complement(5662..5811)
                     /gene="acrZ"
                     /locus_tag="ACHY5W_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFS4447407.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5940..6728
                     /gene="modE"
                     /locus_tag="ACHY5W_002526"
     CDS             5940..6728
                     /gene="modE"
                     /locus_tag="ACHY5W_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFS4447408.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6796..8268
                     /gene="modF"
                     /locus_tag="ACHY5W_002527"
     CDS             6796..8268
                     /gene="modF"
                     /locus_tag="ACHY5W_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFS4447409.1"
                     /translation="MLSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KKELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8530..9546
                     /gene="galE"
                     /locus_tag="ACHY5W_002528"
     CDS             8530..9546
                     /gene="galE"
                     /locus_tag="ACHY5W_002528"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4447410.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKSVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9556..10602
                     /gene="galT"
                     /locus_tag="ACHY5W_002529"
     CDS             9556..10602
                     /gene="galT"
                     /locus_tag="ACHY5W_002529"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFS4447411.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10606..11754
                     /gene="galK"
                     /locus_tag="ACHY5W_002530"
     CDS             10606..11754
                     /gene="galK"
                     /locus_tag="ACHY5W_002530"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4447412.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNNFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALLPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11748..12788
                     /gene="galM"
                     /locus_tag="ACHY5W_002531"
     CDS             11748..12788
                     /gene="galM"
                     /locus_tag="ACHY5W_002531"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFS4447413.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQVQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPLDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHLWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRAGEE
                     YSSLTEYQFIAE"
     gene            12990..13742
                     /gene="gpmA"
                     /locus_tag="ACHY5W_002532"
     CDS             12990..13742
                     /gene="gpmA"
                     /locus_tag="ACHY5W_002532"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4447414.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13901..14953)
                     /gene="aroG"
                     /locus_tag="ACHY5W_002533"
     CDS             complement(13901..14953)
                     /gene="aroG"
                     /locus_tag="ACHY5W_002533"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFS4447415.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15269..15649
                     /gene="ybgS"
                     /locus_tag="ACHY5W_002534"
     CDS             15269..15649
                     /gene="ybgS"
                     /locus_tag="ACHY5W_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFS4447416.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15763..16704
                     /gene="zitB"
                     /locus_tag="ACHY5W_002535"
     CDS             15763..16704
                     /gene="zitB"
                     /locus_tag="ACHY5W_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFS4447417.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16701..17420)
                     /gene="pnuC"
                     /locus_tag="ACHY5W_002536"
     CDS             complement(16701..17420)
                     /gene="pnuC"
                     /locus_tag="ACHY5W_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4447418.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILIFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17458..18501)
                     /gene="nadA"
                     /locus_tag="ACHY5W_002537"
     CDS             complement(17458..18501)
                     /gene="nadA"
                     /locus_tag="ACHY5W_002537"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFS4447419.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18779..18904)
                     /locus_tag="ACHY5W_002538"
     ncRNA           complement(18779..18904)
                     /ncRNA_class="other"
                     /locus_tag="ACHY5W_002538"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         4761 a         4964 c         4734 g         4493 t
ORIGIN      
        1 gagtaataat gaagcatgaa ttatcaagta tgaaggcatt tgtcatactg gcagagtcca
       61 gttcatttaa caatgccgct aaattactca acattacgca acctgcatta acgcgaagaa
      121 taaaaaagat ggaggaagat ttacatattc aactatttga gcgtacaact cgcaaggtta
      181 cgttaacaaa agcaggaaaa aggttgctcc cggaggcgcg ggaattaata aagaaattcg
      241 atgaaacgct ttttaatatt cgtgatatga atgcttatca tcgtggtatg gtgacgttag
      301 cgtgtattcc gaccgcagta ttttattttt taccgctggc aatcggtaaa tttaatgagc
      361 tgtatcccaa tattaaagtg cggattctgg aacaaggcac gaataattgc atggaatcgg
      421 agctatgtaa cgaatcagac tttggcataa acatgaacaa cgtcacaaat tcatccattg
      481 attttagccc actggtcaac gagccgttcg tgctggcctg tcggcgtgac catccattgg
      541 ccaaaaagca gcttgtagaa tggcaggagt tagtgggtta caaaatgatt ggcgtacgct
      601 cctccagcgg caaccgactg ttgatagaac aacagctagc cgacaagccc tggaagctgg
      661 actggtttta cgaagtgcgt catctttcga cgtcattagg actggtggag gcaggactgg
      721 ggatttcagc gcttcctggt ctggcaatgc cccatgcgcc ctattcttcg attattggta
      781 tcccactggt ggaacccgtt atacgtcgga cattggggat tattcgccgc aaagatgccg
      841 tgctttctcc ggcagctgaa cgcttttttg ccttgctgat taatttgtgg actgacgata
      901 aagacaattt gtggaccaat atcgtcgaac gccagagaca tgcgttacag gagataggtt
      961 aatacaccgg cgcaggagag actgcgccgg gtaaatcagc ggttagtgtg cgttaaccac
     1021 cacccacatt ggtccctgcc cgaccgcgta gcggcctttt tcatgcagta gcccctgctc
     1081 accaacaatt tcgtataccg agatgtggtg cgatttttgc ccggcggcaa tcagatactt
     1141 accacggtga tcgatattaa agccgcgcgg ctgggtttcc gttggctgga agccctcttt
     1201 actcaacacg ctgccatctt ccgaaacgct gaagacagta atcaggctgg cggtacggtc
     1261 acaggcatat aagtgacgac catccggggt aatatgaata tcagccgccc aacgggtgtc
     1321 ggagaagttt tccggcatca tatccagcgt ctggacacat tcgatattgc cgtgcggatc
     1381 tttcagttcc cagacatcca ctgagctgtt taactcattg acgcaatacg catattgttc
     1441 gtttggatgg aataccatat gacgcgggcc ggccccttca acggtagtca cttccgcagg
     1501 gtcctgcgcc accagatgac catcatcgct gaccgtaaac aggcaaatgc gatcctgctt
     1561 taatgccgga acccacagcg tacggttgtc cggtgagata ttggcggaat ggcaaccgtc
     1621 cagcccctcg accacatcga cgacgccaac tggcaggcca tcttccagac gcgttacgct
     1681 cacgttaccc gcattgtaag aacctacaaa gacaaactgc ccctggtgat cggtggaaat
     1741 atgcgtcgga ctacccggca gcgcagactc tgcggcaaag gtcagtgcgc catcgtccgg
     1801 ggcgatacga tacgccagga cgcgaaactc agggcgaaca ccaacataga gataacgttt
     1861 gtccgggctg accaccatcg gctgcacctg ccccggcaca tcgacaacct gtgtcagcgt
     1921 cagtgcgcct tcatgattca ggttccagac gtgaatttgc tggctctcag ggctggcgat
     1981 ataaactgtt tgcttcatga atgctccttt gcatttagca actgactgta acagctaaaa
     2041 ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg atagtggtat
     2101 cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg ccatgaccac
     2161 acgcgtgatt gctctcgact tagacggcac cttattgacc ccgaaaaaga ccctgcttcc
     2221 ttcatcgata gaagccctga cccgcgctcg cgaagcaggc tatcaattaa tcatcgtcac
     2281 aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg atacacctgc
     2341 tatttgctgt aatggcacct atttgtatga ttatcatgca aaaaccgtgc tggaagcgga
     2401 cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac accacattca
     2461 cggtctgatg tatgtggatg atgcaatggt ctatgagcac ccgaccgggc atgtcattcg
     2521 cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca cacaagtcgc
     2581 ttctctggct gaaacggcgc aacaagttaa cgccgtatgg aagttcgccc tgacgcacga
     2641 tgacctgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg gactggagtg
     2701 tgaatggtcc tggcacgatc aggttgatat tgcacgcggc ggcaacagca aaggtaaacg
     2761 tttgacgaaa tgggttgagg cgcaaggctg gtcgatggaa aacgtcgtgg cgttcggcga
     2821 taactttaat gatatcagta tgctggaggc cgctggtaca ggtgtggcga tgggcaacgc
     2881 cgatgacgcg gtaaaagcgc gcgccaacat tgtgattggt gataacacca ccgacagcat
     2941 tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca cttttaattt
     3001 gcgcgtacag ccacaggcca ggtttgatcg ccagttcatc cctggcccac gggctgatac
     3061 gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctggccg ttgtcgtcat
     3121 aactattaac aacttttgcc cgcaatacgt tacgaatgct ggtttgctgc ggcggttgta
     3181 aaaccagaga aacatcggaa gcctgaatgc ggatacgtag cgcagcttgc agcggttcgt
     3241 ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcggtcatc gcgtaatgcg
     3301 gatgatgctc aagcaccgtc actttcagaa tgctactttg ttgctctttc ggcagccacg
     3361 gattcatcac gctactaccc cagacttctt ccagcgcgcc aaaggctttc acctgaccgt
     3421 tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa tggctgacat
     3481 acaacatcgg gatgttgatt tcccgcgtca gacgttgcag ataaggcaac agttcgcgtt
     3541 tccgcggaat atccagtgac gccagcggtt catccagcaa cagcaattct ggtgctgtca
     3601 gcaaagcccg cccaatcgct acgcgctgtt tttcgcctcc ggacaggctg cctggtaaac
     3661 ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga tcgaccatgc
     3721 ttttcgccat gccgtagcgc agattgccac gcactttgta atgcggaaac agccgcgcat
     3781 cctgaaaaac atagccaacg cgacgttttt ctggcgtcag gcagatacct ttatccgcgt
     3841 catttagtac ccgaccattg aggacaatcc tccctttttg cgggcgcgtc agtccactga
     3901 tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca gtgatgccat
     3961 tggcgggcag cgtttcatta atagtcaggc aatggttgcc caacgtctgg gaaaaattca
     4021 gttccagcat gattagcgcc ccgcccgttc acggctgatt ctggccaacc attctgaaac
     4081 caacagggag atcatcgcca gcgcaataga aataatgcac agtctcgctg cgccactttc
     4141 cccgccgggg gtctgaatca gggtatacat ggcagaagga atggttcgcg tttcaccagg
     4201 aatgttcgac acaaaggtga tggttgcgcc aaattcaccg agagaacgag caaaagccag
     4261 taccgtacca acaataattc ccggtaaggt cagcggtaac gtgatagtaa agaaaacgcg
     4321 ccagcgcccg gcccccagtg ttcttgcggc ctgttccagt ttgacatcaa ccccttccag
     4381 cgccagacga attgcccgca ccatcagcgg aaacgacatg acggcggcag cgagaaccgc
     4441 gccgcgccag ctaaaggcga aggttatacc aaaccagtca tacagacgtt caccgataaa
     4501 tccgcgccgc cccatcgaaa ctaataataa gtaaccgacg accacgggcg gtaacaccag
     4561 cggtagatgc agtacgctgt cgagcagaga tttgcccgga aacgtgcaac gcaccagtaa
     4621 ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag aaactttcag
     4681 gcttaataaa actgcctgcc attctggatc ggtcagtatc attacttggt tgtaaatccg
     4741 taacgtttaa agatttccgc agcctgcggt cccttcagat aatcataaaa tgctttcact
     4801 gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt atgtgaatct
     4861 tccgggaagg tggcaaccac ttttacccct ttgctggcaa ctgcgtcaga accgtaaaca
     4921 atgcccaacg gcgcttcatt gcgttcgacc aacgccagcg cgccacgaac atcttccgcc
     4981 ggagccagtt tcggggagag cgtatcccat gcgcccagtt tttgcagtgc ttcttttgca
     5041 taaatgccag cagggacatg ttccggatcg ccaaccgcga ggcgaccgcc attcagcagc
     5101 gaagtccagt tagttttgct gtcgatgatg aaatctttct gctcgctggc tttcggtgct
     5161 ataacgacca ggctattgcc gagcagtgtc tgacgcgtag ctgtatcgat cgctttttta
     5221 tcaaccgcat aatccatcca tttctgatcg gcagaaataa acagatccgc aggcgcaccc
     5281 gcttcaatct gacgggcgag agtagatgac gaagcgaaag aagaaaccac atccacgcct
     5341 ttctcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga tgcggcggcg
     5401 aacaccgtga ttttcccttc atctgctagt gcattgccag caacagcgaa agagagtgcc
     5461 gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat gtaacgttat
     5521 gtaggcgaca atataacgac taactcggaa ttttcccagc agttattgct aacctttgtg
     5581 aggtagataa gaaaaaatat cggcaggaaa agcaggaagt tgagaaaaag aaaatgcccg
     5641 actaagcggg cattcaggga atcaatgatt ttgtccgggc tggtcttttt taccaacacc
     5701 agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga tggccatcac
     5761 gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca tggtcaactc
     5821 cagatatagt catgagatta ttctaaccgc taagctcaga aaagcactcc ccttttgtgc
     5881 ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag gacattgtta
     5941 tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc gacccgcgcc
     6001 gcatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga gcgaaagatg
     6061 ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag ttaagtgagc
     6121 atattctggt cgagcgcgca acaggcggta aaggtggcgg cggcgcagta ctgacccgct
     6181 atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa aaagcctttg
     6241 atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg atctcacgtt
     6301 tttcactgca aaccagcgcc cgtaaccagt ggttcggtac catcaccgcc cgcgatcatg
     6361 atgacgttca acagcatgtt gatgtcttac tggctgatgg aaaaacacgc ctgaaagtcg
     6421 caattaccgc acaaagcggc gcgcgtctgg ggctggatga aggcaaggaa gtgttgatat
     6481 tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcgcaa aacgctgaca
     6541 accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc gaagtattaa
     6601 tggcgctacc ggacgggcaa acactgtgcg ccacagtgcc ggtaaatgaa gcgacttctc
     6661 ttcagcaagg acagaatgtc acggcctact ttaatgccga cagcgtgatt atcgccacgc
     6721 tgtgctaagc gtgttgacaa tttgttatga aacacgtatc cctgtcagta atcgctgcac
     6781 aaaatgggat ataaaatgtt atcgttgcaa attttgcaag gcacatttcg tcttagcgac
     6841 acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg ggcgtttgtc
     6901 ggttcgaatg gaagcgggaa atcggccctg gcccgcgcgc tggcggggga acttccgctt
     6961 ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt cgagcaattg
     7021 caaaagctcg tcagcgacga atggcagcgg aataacaccg atatgctcgg ccctggcgaa
     7081 gatgacaccg gacgcactac ggctgagatt attcaggatg aagtaaagga tgcaccgcgc
     7141 tgcatgcaac tggcacagca gttcggtatt accgccctcc tcgaccgacg ctttaaatac
     7201 ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc ggagcctgac
     7261 ttgttgattc ttgatgagcc gttcgatggc ctggatgttg cctcacgtca gcagctggct
     7321 gagctactcg cctcgttaca tcagtccggt attactctgg tactggtgct caatcgcttc
     7381 gatgagatcc cggaatttgt ccagtttgct ggcgtgctgg cggattgtac gttagcggaa
     7441 actggcgcta aaaaggaact gctccaacaa gcactcgtcg cgcaactggc gcatagtgaa
     7501 cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca cgccttaccc
     7561 accaacgaac cgcgcattgt gctgaataat ggcgtggttt cttataacga tcgccccatt
     7621 cttaataacc ttagctggca ggtgaatcct ggcgagcact ggcaaattgt cgggccaaat
     7681 ggtgcgggaa aatcgacgtt attaagcctg attactggcg atcatccgca aggttacagc
     7741 aacgatttga cgcttttcgg ccgacgtcgc ggcagcggcg aaaccatctg ggacatcaaa
     7801 aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag taccacggtg
     7861 cgtaatgtga ttctttctgg ctatttcgat tcgattggca tttatcaggc cgtttcggat
     7921 cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa acgcacggct
     7981 gacgctccgt tccatagtct ttcctgggga cagcaacgtc tggcgctgat tgtccgcgca
     8041 ctggtgaaac atccgacgtt gcttattctc gatgaaccac tacaggggct tgatccgctg
     8101 aatcgccagc ttatccgccg ttttgttgat gtgctgatta gcgaaggtga aacgcaattg
     8161 ttgtttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg tcttgagttc
     8221 gtgccggacg gtggactcta tcgctatgtg ctgacaaaaa tatattgagt cggtagtgct
     8281 gaccttgccg gaggcggcct aagcgccctc tccggccaac ggttcgacgc atgcaggcat
     8341 gaaaccgcgt ctttttttca gataaaaagc gcaatcattc ataaaccctc tgttttataa
     8401 tcacttaatc gcacataaaa aatggctaaa ctcttgtgta aacgattcca ctaatttatt
     8461 ccatgtcaca cttttcgcat ctttgttatg ctatggttat ttcataccat aagcctaatg
     8521 gagcgaatta tgagagttct ggttaccggt ggtagcggtt acattggaag tcatacctgc
     8581 gtgcaactac tgcaaaacgg tcatgatgtc atcattcttg ataacctctg taacagtaag
     8641 cgcagcgtac tgcctgttat cgagcgttta ggcggcaaac atccaacatt tgttgaaggc
     8701 gatatccgta acgaagcgtt gatgaccgag atcctgcacg atcacgctat cgacaccgtg
     8761 atccacttcg ccgggctgaa agccgttggc gaatcggtac aaaaaccgct ggaatattac
     8821 gacaacaatg tcaacggtac tctgcgcctg attagcgcca tgcgcgccgc taacgtcaaa
     8881 aactttattt ttagctcctc cgccaccgtt tatggcgatc agcccaaaat tccatacgtt
     8941 gaaagcttcc cgaccggcac accgcaaagc ccttacggca aaagcaagct gatggtggaa
     9001 cagatcctca ccgatctgca aaaagcccag ccggactgga gcattgccct gctgcgctac
     9061 ttcaacccgg ttggcgcgca tccgtcgggc gatatgggcg aagatccgca aggcattccg
     9121 aataacctga tgccatacat cgcccaggtt gctgtaggcc gtcgcgactc gctggcgatt
     9181 tttggtaacg attatccgac cgaagacggt actggcgtac gcgattacat ccacgtaatg
     9241 gatctggcgg acggtcacgt cgtggcgatg gaaaaactgg cgaacaagcc aggcgtacac
     9301 atctacaacc tcggtgctgg cgtaggcagc agcgtgctgg acgtggttaa tgccttcagc
     9361 aaagcctgcg gcaaatcggt taattatcat tttgcaccgc gtcgcgaggg cgaccttccg
     9421 gcctactggg cggacgccag caaagccgac cgtgaactga actggcgcgt aacgcgcaca
     9481 ctcgatgaaa tggcgcagga cacctggcac tggcagtcac gccatccaca gggatatccc
     9541 gattaaggaa cgaccatgac gcaatttaat cccgttgatc atccacatcg ccgctacaac
     9601 ccgctcaccg ggcaatggat tctggtttca ccgcaccgcg ctaagcgccc ctggcagggg
     9661 gcgcaggaaa cgccagccaa acaggtgtta cctgcgcacg atccagattg cttcctctgc
     9721 gcaggtaatg tgcgggtgac aggcgataaa aaccccgatt acaccgggac ttacgttttc
     9781 actaatgact ttgcggcctt gatgtctgac acgccagatg cgccagaaag caacgatcct
     9841 ttgatgcgtt gccagagcgc gcgcggtacc agccgggtga tctgcttttc accggatcac
     9901 agtaaaacgc tgccagagct cagtgttgcg gcattgacgg aaatcgtcaa aacctggcag
     9961 gagcaaaccg cagagctggg aaaaacgtac ccgtgggtgc aggtctttga aaacaaaggt
    10021 gcggcgatgg gctgctctaa cccgcatccg cacggacagg tttgggcaaa tagcttcctg
    10081 cctaacgaag ctgagcgcga agaccgcctg caaaaagaat attttgccga gcagaaatca
    10141 ccaatgctgg tggattatgt tcagcgcgag ctggcagacg gtagccgtac cgttgtcgaa
    10201 accgaacact ggttagccgt agtgccttac tgggctgcct ggccgttcga aacgctactg
    10261 ctgcccaaag cccacgtttt acggatcacc gatttgaccg acgcccagcg cagcgatctg
    10321 gcgctggcgt tgaaaaagct gaccagtcgt tatgacaacc tcttccagtg ctccttcccc
    10381 tactctatgg gctggcacgg cgcgccattt aatggcgaag aaaatcaaca ctggcagctg
    10441 cacgcgcact tttatccgcc tctgttgcgc tccgccaccg tacgtaaatt tatggttggt
    10501 tatgaaatgc tggcagaaac ccagcgagac ctgaccgcag aacaggcagc agagcgtttg
    10561 cgcgcagtca gcgatatcca ttttcgcgaa tccggagtgt aagaaatgag tctgaaagaa
    10621 aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcatac cattcaggcg
    10681 cctggccgcg tgaatttgat tggtgaacac accgactaca acgacggttt cgttctgccc
    10741 tgcgcgattg attatcaaac cgtgatcagc tgtgcgccac gcgatgaccg taaagttcgc
    10801 gtaatggcag ccgattatga aaatcagctt gatgagtttt cccttgatgc gcccattgtc
    10861 gctcatgaaa actatcaatg ggctaactac gttcgtggcg tggtgaaaca tctgcaactg
    10921 cgtaacaaca acttcggcgg cgtggacatg gtgatcagcg gcaacgtgcc gcagggtgcc
    10981 gggttaagtt cttccgcctc actggaagtg gcggtcggaa ccgtattgca gcagctttat
    11041 catctaccgc tggacggcgc acaaatcgcg cttaacggtc aggaagcaga aaaccagttt
    11101 gttggctgta actgcgggat catggatcaa cttatttccg cgctcggcaa aaaagatcac
    11161 gccttgctga ttgactgccg ctcactgggt accaaagcag tttccatgcc gaaaggtgtg
    11221 gctgtggtca tcatcaacag taacttcaaa cgtaccctgg ttggcagcga atacaacacc
    11281 cgtcgtgaac agtgcgaaac cggtgcgcgt ttcttccagc agccagccct gcgcgatgtc
    11341 accattgaag agttcaacgc tgttgcgcat gaactggacc caatcgtggc gaaacgcgtg
    11401 cgtcatattc tgactgaaaa cgcccgcacc gttgaagctg ccagcgcgct ggagcaaggc
    11461 gacctgaaac gtatgggcga gttgatggcg gagtctcatg cctctatgcg cgatgatttc
    11521 gaaatcaccg ttccgcaaat tgacactctg gtagaaatcg tcaaagctgt gattggcgac
    11581 aaaggtggcg tacgcatgac cggcggcgga tttggcggct gtatcgtcgc gctgctcccg
    11641 gaagagctgg tgcctgccgt acagcaagct gtcgctgaac aatatgaagc aaaaacaggt
    11701 attaaagaga ctttctacgt ttgtaaacca tcacaaggag caggacagtg ctgaacgaaa
    11761 ctcccgcact ggcacccgat ggtcagccgt accgactgtt aactttgcgt aacaacgcag
    11821 ggatggtagt cacgctgatg gactggggtg cgactttact ttcagcccgt attccgcttt
    11881 ccgatggcag cgtccgcgag gcgctgctcg gctgtgccag cccggaatgc tatcaggttc
    11941 aggccgcgtt tctgggggcc tctattggtc gttatgccaa ccgcatcgcc aatagccgtt
    12001 atacctttga cggtgaaacc gtgacgcttt cgccaagtca gggcgtcaac caactgcacg
    12061 gcggcccgga agggttcgac aaacgtcgct ggcagattgt gaaccagaac gatcgtcagg
    12121 tgctgtttgc cctgagttca gatgatggcg atcagggctt cccgggtaat ctcggcgcga
    12181 cagtgcaata tcgtctgacc gacgataacc gtatctccat tacttatcgc gccacagttg
    12241 ataaaccttg cccggtgaat atgactaatc acgtctattt caatctcgac ggcgagcagt
    12301 ctgacgtgcg caatcacaag ttgcagattc tggcggacga atatctgccg gttgatgaag
    12361 gcggcattcc cctcgacggc ctgaaatctg tcgccggaac atcttttgat ttccgcaatg
    12421 ccaaaatcat cgccagtgag tttcttgccg acgacgatca gcgcaaagtg aaaggttacg
    12481 atcacgcgtt cttgttacag gccaaaggcg atggcaagaa agtggcggca catctctggt
    12541 cagcagatga aaaattgcag ttgaaggtct acaccaccgc tccggctctg caattctact
    12601 ccggcaactt cctcggcggc acaccgtcgc ggggaaccga accttacgcc gactggcaag
    12661 ggctggctct ggaaagcgag tttctaccgg acagcccgaa ccaccctgaa tggccgcaac
    12721 cggactgctt tctccgtgct ggcgaagagt attccagcct gacggaatat cagtttattg
    12781 ctgagtaatg ttaagccctc gtcatgaggg ctttatctca tattgttcaa atcaccagca
    12841 aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgcc actattttcg
    12901 ctatggttat gcgtaagcat tgctgttgct tcgtcacagc aatataatga gaattattat
    12961 cattaaaaga tgatttgagg agtaagtata tggctgtaac taagctggtt ctggttcgtc
    13021 atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac gtggatctgt
    13081 ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag gaaggttaca
    13141 gctttgactt tgcttacacc tctgtgctga aacgcgctat ccataccctg tggaatgtgc
    13201 tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg aacgaacgtc
    13261 actacggtgc gctgcagggt ctgaacaaag cggaaactgc tgaaaagtat ggcgacgagc
    13321 aggtgaaaca gtggcgtcgt ggttttgccg tgactccgcc ggaactgact aaagatgatg
    13381 agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagag ctgccgctga
    13441 cggaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa accattctgc
    13501 cgcgtatgaa gagcggtgag cgcgtaatca tcgctgcaca tggtaactcg ttacgtgcgc
    13561 tggtgaaata tctcgataac atgagcgaag aagagattct tgagctgaat attccgactg
    13621 gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc tattatctgg
    13681 gtaatgctga cgagatcgcg gcgaaagcag cagccgttgc gaaccagggt aaagcgaagt
    13741 aaacgtcatt cgtttaaaaa tgagaaagcc gactgcaagt gagtcggctt ttttgttgct
    13801 aacaatggag cacattgcct gatgcgacgc tgcgcgtctt atcaggccta cggtgattca
    13861 tcggatacgc cactctgacg gcgcatccga caattaaact ttacccgcga cgcgctttta
    13921 ctgcattcgc cagttgacgt aacagagcat cggtatcatc ccagccaatg caggcatcgg
    13981 tgatactctt accgtaggcc agcggctccc cgctctcgag gctctgattg ccttccacca
    14041 gatggctttc caccatcacg ccaataatgg ccttttcgcc accggcaatc tgctggcaaa
    14101 catcagcaca aacatccatc tgctttttga attgttttga cgagttagca tggctgaaat
    14161 cgatcatcac ctgagctggc agacctgctt tgttcagccc ttctttcact tcagcaacgt
    14221 gcttcgcgct gtagttaggc tctttaccgc cgcgcagaat gatatggcaa tcgccgttac
    14281 cgctggtatt cacaatcgcc gaatgccccc atttcgttac ggacaggaag cagtgcggcg
    14341 caccggcggc attaatggca tcgatagcca ctttaatcgt accgtctgtg ccatttttaa
    14401 aacctaccgg acaagaaaga ccagacgcca gttcgcggtg cacctgcgat tccgtggtac
    14461 gtgcaccaat tgcgccccag ctcatcaggt cagcgagata ctgaggagtg atcatatcga
    14521 ggaattcacc cgcagctggc agaccgctgt cgttaatatc gagcagcaat ttacgggcaa
    14581 tacgcaaacc gtcgttgatc tggaagctgt tatccatatg cggatcgtta atcagccctt
    14641 tccagcccac cgtggtccgc ggcttttcaa aatagacgcg catcacgatt tccagctcat
    14701 cttgcagctc ttcacgtagc gccagcaagc gagtggcata ctctttagcc gcgacaggat
    14761 catgaattga gcacgggcca atcaccacca acaggcgatc atcattacct ttcaggatct
    14821 tatggatcgc ttttcgggca tgggcaaccg tattcgcggc attttcagta gcggggaatt
    14881 tttccagcaa tgcgacagga ggaagtaact ctttgatttc tttgatgcgt aaatcgtcgt
    14941 tctgataatt catgtctgtt ccagtgttgc catacttatc ttagtgaatg caatacttcc
    15001 aatctatatc ttccgtcaga atgtgtaaac ggggttttac actatgaacg gattaatcct
    15061 ggaattagca agaaaaacgc cagattgtcg cgaaaaacga gatctctcct acaatttcta
    15121 actgtaactc ctttgtttgt taattatttc aagattctct gctgcgtttc ataacctggc
    15181 tgaaatctta aaccaatgcc ttatattcac ctgcaaatgc actgttggaa gaggttatcc
    15241 gacataacga ccataacagg agcatcctat gaaaatgaca aaactggcca cactatttct
    15301 gactgccact ctaagccttg ccagcggtgc cgcactggct gccgatagcg gagcgcaaac
    15361 taataacggc caggcaaacg ccgcagctga tgcgggccag gtagcccctg acgcccgtga
    15421 aaatgtcgcg ccaaataacg tcgacaataa cggggtaaat accggttctg gcggcacaat
    15481 gctgcattcg gatggttctt caatgaacaa tgacggaatg accaaagatg aagagcacaa
    15541 gaacaccatg tgcaaagatg gtcgctgtcc ggatatcaat aaaaaggtac aaaccggtga
    15601 tggcatcaat aatgatgtcg ataccaaaac cgacggtacc acgcagtaac cctgcaaaat
    15661 agccaataat gggagagcgg agtctctccc ttttaatttt tcacttctaa aagcagtatg
    15721 atgtcataaa gttaagtgag aataattaag ggatgactcg tcatggcgca ctcacactca
    15781 cacacgtcct cacacctgcc agaagataat aatgctcgtc gcttgttgta tgctttcggc
    15841 gtgactgccg ggtttatgct ggtagaagtc gttggtggtt ttctttctgg ttctctggca
    15901 ttgctggccg atgcgggtca tatgttgacc gatactgctg ccctgctttt cgccctactc
    15961 gccgtgcaat tttcacgtcg acctcccacc attcgccaca ctttcggttg gctaagattg
    16021 accacgctcg cggcgtttgt gaatgccatc gccctggtgg tgattaccat tttgattgtc
    16081 tgggaggcga tagaacgctt ccgcacaccg cgtccggtcg agggcggcat gatgatggca
    16141 attgccgtgg ctgggttgct ggcaaatata ctttctttct ggttacttca tcacggcagc
    16201 gaagagaaaa acctcaacgt gcgcgcagcg gcactgcatg tgctcgggga cctgctgggt
    16261 tcggttgggg ctattattgc cgcgttgatt attatctgga ccggctggac acctgctgac
    16321 cccattctct cgatactggt gtcgcttctg gttctgcgta gcgcgtggcg actgttgaaa
    16381 gatagcgtga atgaattact tgaaggtgca ccggtatcgc tggatatcgc tgaactgaag
    16441 cgtcgtatgt gccgggaaat cccggaagtc cgcaatgtgc accatgtgca tgtatggatg
    16501 gtaggcgaga agccggtgat gacgttgcat gtgcaggtga tcccgccaca cgatcacgat
    16561 gccttgttgg atcagatcca acactacctg atggatcact atcagattga gcacgccacc
    16621 attcagatgg aatatcaacc ttgtcatggg ccagactgcc acctgaatga aggggtgtct
    16681 ggccattcac atcatcacca ttaatgggac agcgcgcgtg agcctctttc acgtgcgctg
    16741 ttgatccaca tccggctgcc gttgagcgca ataaagatca ggatgatgta ctccagagac
    16801 atggcgtaaa cgccctgaag tgcaaaaata acgacgctaa tcacgttaat aatcacccac
    16861 aacagccagt tttccacata cttacgcgtc atcagaatca ttgccacgat agataacacc
    16921 atcatgcatg aatcccagaa cgggaaagcg tccggttgca gttcaggcat cacaacctgt
    16981 aatcctaatg cttgcatgat catgactgcc acgcgggtca aaaatgcaaa caccggattg
    17041 ataaataccg tcatcagacc aatcgaaaca acgcaaaccg ccaaccagct tagcgccttc
    17101 ggcaatggca accagcgaat tttcaactcc gcctcgttct gactggtttg tcgcgaccac
    17161 gcataccaac cgtaaatatt cgcggcaaag aaaaacacct gtaatagcag gctggcatac
    17221 agctgaatct gaaagaaaat aatgccaaac aaggtgacgt tgatcaggcc aaagaagtag
    17281 ttgctgatct tctccagact ggcaaggcca atacacagca acccggcgat cgtgcctacc
    17341 gcttcgatcc atgagagatc ataaccgcct gctcctattg gtatatgtac caggatattc
    17401 tgcacactaa aaaaatccat ctttccccca aagcgtaacg ttacgcctta ttattcgtta
    17461 tccacgtagt gtagccgcaa aatccagcat acgattgagc ggcaccagcg ccctctctcg
    17521 cagcctttca tcaacatgaa cctcgtgatt gcttccttcc tgttctaatg cctctgcgat
    17581 ggcctgaagg ccattcatgg ccatccacgg acaatgcgcg cagctgcggc aggttgcacc
    17641 ctcacctgcg gttggtgctt ccagtaactc tttatctggc accgcctgct gcattttgta
    17701 gaaaataccc cgatcggttg ccacgataag ccgctgatgt ggcaatgttt ttgcagcagc
    17761 gatcagttga ctggtggaac cgaccgcatc cgccatatcg acaatagctt gtggtgattc
    17821 tggatgcacc agtatggcag catccgggta ttcttcttgc aagcgggtta acgcctgagt
    17881 cttaaattca tcatgcacaa tgcaagcacc ctgccagcat aaaatgtctg cgcccgtctg
    17941 tttttgcacg taacgcccca gatgtttgtc gggtgcccag atgatttttt cacccaaact
    18001 atcaagatga tcaataagtt cgacggcaat gcttgaagtt accacccaat ctgcgcgcgc
    18061 ttttaccgca gcagaagtgt tggcgtagac gacgacagta cgatcgggat gggcatcgca
    18121 aaatgcgtta aattcttcaa cagggcagcc gagatccagt gaacactcag cctgaagtgt
    18181 cggcatcaga attgtttttt ccggactgag aattttggcg gtttctccca taaatctcac
    18241 cccagcgact aacaacgtag aagcgggatg ctttgcaccg aagcgcgcca tttccaaaga
    18301 atcagaaata cagccaccgg tttcttctgc cagttgttga atttcgggat cggtatagta
    18361 atgggcaacc atcaccgcat tacgttcttt tagcagacgt tttatcttct cgcggtaata
    18421 cgctttttca tcaatgctta acggcgtcgg cttcgggggg aaaggataaa tcgccgtgtc
    18481 tggatcaaac atcacgctca tcttaccatc tcgttttaca ggcttaacgt taaaaccgac
    18541 ataagcgtca tattcgctgt tatcggtgta atttgtttta tatgctaaac aagatagtcg
    18601 aatggtgggg aaaagtcact cgaattacgt atgagatgcc gaatgccact ctttgcatct
    18661 tattcctccg acgtaaagtt atttacgcag caagcgcgga gtatttcatc agggttaaga
    18721 attgaaattg agagatgcct gaatagcaaa aaagcgcctt tagggcgcct tttaacacgg
    18781 gtgggtcgtg caggatgact cgcttcgctc gcccttcggg ccgtcgccgc gagcggctcc
    18841 gttgtttcgc tgcgctcaac tcaaacctgc tgcaggttcg aatctttgag tatcgcagat
    18901 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg at
//
LOCUS       DAZUGK010000085        18512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZUGK010000085 DAZUGK010000000
VERSION     DAZUGK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.085"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(9..632)
                     /gene="rsmG"
                     /locus_tag="ACHY5W_002539"
     CDS             complement(9..632)
                     /gene="rsmG"
                     /locus_tag="ACHY5W_002539"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4447420.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(696..2585)
                     /gene="mnmG"
                     /locus_tag="ACHY5W_002540"
     CDS             complement(696..2585)
                     /gene="mnmG"
                     /locus_tag="ACHY5W_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4447421.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2964..3407)
                     /gene="mioC"
                     /locus_tag="ACHY5W_002541"
     CDS             complement(2964..3407)
                     /gene="mioC"
                     /locus_tag="ACHY5W_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFS4447422.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3497..3955)
                     /gene="asnC"
                     /locus_tag="ACHY5W_002542"
     CDS             complement(3497..3955)
                     /gene="asnC"
                     /locus_tag="ACHY5W_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFS4447423.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4107..5099
                     /gene="asnA"
                     /locus_tag="ACHY5W_002543"
     CDS             4107..5099
                     /gene="asnA"
                     /locus_tag="ACHY5W_002543"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFS4447424.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5104..6555)
                     /gene="viaA"
                     /locus_tag="ACHY5W_002544"
     CDS             complement(5104..6555)
                     /gene="viaA"
                     /locus_tag="ACHY5W_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFS4447425.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQIMDLLHRL
                     NSPWAEQARQLVDTNSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSASQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6549..8045)
                     /gene="ravA"
                     /locus_tag="ACHY5W_002545"
     CDS             complement(6549..8045)
                     /gene="ravA"
                     /locus_tag="ACHY5W_002545"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFS4447426.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRYLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8268..10136
                     /gene="kup"
                     /locus_tag="ACHY5W_002546"
     CDS             8268..10136
                     /gene="kup"
                     /locus_tag="ACHY5W_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFS4447427.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10303..10722
                     /gene="rbsD"
                     /locus_tag="ACHY5W_002547"
     CDS             10303..10722
                     /gene="rbsD"
                     /locus_tag="ACHY5W_002547"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4447428.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10730..12235
                     /gene="rbsA"
                     /locus_tag="ACHY5W_002548"
     CDS             10730..12235
                     /gene="rbsA"
                     /locus_tag="ACHY5W_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFS4447429.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12240..13205
                     /gene="rbsC"
                     /locus_tag="ACHY5W_002549"
     CDS             12240..13205
                     /gene="rbsC"
                     /locus_tag="ACHY5W_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFS4447430.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13230..14120
                     /gene="rbsB"
                     /locus_tag="ACHY5W_002550"
     CDS             13230..14120
                     /gene="rbsB"
                     /locus_tag="ACHY5W_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFS4447431.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14246..15175
                     /gene="rbsK"
                     /locus_tag="ACHY5W_002551"
     CDS             14246..15175
                     /gene="rbsK"
                     /locus_tag="ACHY5W_002551"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4447432.1"
                     /translation="MQNAGSLVVLGSINADHILNLHSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPVLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15179..16171
                     /gene="rbsR"
                     /locus_tag="ACHY5W_002552"
     CDS             15179..16171
                     /gene="rbsR"
                     /locus_tag="ACHY5W_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFS4447433.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16149..17564)
                     /gene="mdtD"
                     /locus_tag="ACHY5W_002553"
     CDS             complement(16149..17564)
                     /gene="mdtD"
                     /locus_tag="ACHY5W_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFS4447434.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVP"
     gene            complement(17587..18279)
                     /gene="yieP"
                     /locus_tag="ACHY5W_002554"
     CDS             complement(17587..18279)
                     /gene="yieP"
                     /locus_tag="ACHY5W_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4447435.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4452 a         4605 c         4840 g         4615 t
ORIGIN      
        1 gataaaaatt aaattttatt tgctttaatc accaccagat ggcgttcgcc atccagggca
       61 ggaacctgaa gtttaaccac tgattcaacc tgatattctt cgggcaacaa agcgatttca
      121 tcttccggca tttgcccttt cagcgcgtag aaacggcctt gctcaccagg aagatggtga
      181 caccagctca ccatatcgtt cagagaggca aaagcgcggc taattacgcc atcaaatggc
      241 ggctctgaag gaaactcttc taccctgctc tgtactggct caatattctc cagtttaagc
      301 tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa
      361 tgggcttcag gacgcacgat agagagtgga atgccaggca gtccaggacc ggtgccgaca
      421 tcgataaacc gttcaccttg cagatacggt gccaccacaa tgctatcgag aatatggcgt
      481 accagcatct cattaggatc gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc
      541 agcatattca cgtaggcaat aagctggttt ttctggtgat cggtaagcga aatacctgcg
      601 tctttcagca gtaaggagag tttgttgagc acggtgatta cctgttcttg atgcgttacc
      661 tggtaagcgg gtgcttacca ggcattttta atgcgttatg cgctacgacg cagcataccc
      721 tgttttttca gccacaccag cagaatggag atagccgcag gcgtgacgcc agaaatacgt
      781 gaagcctggc cgatagaggc cggtttgtga tcgttaagtt tggcgatcac ttcgttagaa
      841 agaccggata cctggcggta atccagtgtc gcgggtagca gggtgttctc gttacgcagc
      901 tgcttttcga tctcatcttg ctggcgcgcg atataacctt cgtatttaac ctgaatctca
      961 acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat
     1021 ttttcataag tcatttccgg acgacgcagc agatcttcac cactggcttc acgggaaagc
     1081 ggcgcagtca ggtgagcatt cacttcggct gcagcttccg ccgacggggt tacccaggtc
     1141 gatttcagac gctgacgctc acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa
     1201 cgttcgtcat ccaccaggcc cagttcacga ccgatttcag tcaaacgcag atccgcatta
     1261 tcttcgcgca gcatcagacg atattctgcg cgcgaagtaa acatacgata cggttctttg
     1321 gttcctaaag tgcacaggtc atcaaccagt acgccgagat acgcctgaga acgcgccgga
     1381 gcccaacctt ctttgtcagc agacagacgg gcagcgttaa gaccggccag caaaccttgc
     1441 gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg
     1501 ataaacttgc tctccagcgt cggtttcagg tcgcgtggat cgaagaagtc atactcaatg
     1561 gcataacccg gacgcacgat cttcgcgttt tccatcccct gcatggagcg gacgatttgc
     1621 atctgcacat cgaacggcag gctggtggag ataccgttcg gataaatttc gttagaggtc
     1681 agcccttccg gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg catgactttg
     1741 tcttcgatcg acgggcagta gcgtgggccg acaccttcga tcacccctgc gtacattggg
     1801 ctacgatcga ggttactgcg gatcacatca tgggttttct cgttggtatg agtgatataa
     1861 cacggcacct gctggggatg ctgggacgca ttgcccataa acgagaatac cggcattggg
     1921 ttatcgccat gctgttgcgc cagtacgcta aagtcgatgg ttcgagcatc aatacgcggt
     1981 ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc
     2041 ggaatggacg gcggatcacc agcacggcca ccgctgtaat tatccagacc gatatgaatt
     2101 ttaccgtcga ggaacgtccc aacggtgagc acgacggctt tggcacggaa cttcagtccc
     2161 atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa taagatcttc aacagcctgc
     2221 tggaagatca tcaggttcgg ttggttctcc agcgccgtgc gtaccgcctg acggtagagc
     2281 acacgatccg cctgagcgcg ggtagcgcga accgccggtc ctttgcttgc gtttagtatc
     2341 ctaaactgga tacccgcctg atcgatcgct ttcgccatca gaccgccgag tgcatccact
     2401 tcttttacca gatgtccctt cccaataccg ccgatcgccg ggttgcagct catctgcccc
     2461 agagtgtcga tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc cgcggccatc
     2521 gcggcctcgg tgcctgcatg acccccacca atgatgatga cgtcaaaagg atccggataa
     2581 aacatggtga ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg ggccgtggat
     2641 tctactcaac tttgtcggct tgagaaagac ctgggatcct gggtattaaa aagaagatct
     2701 atttatttag agatctgttc tattgtgatc tcttattagg atcgcactgc cctgtggata
     2761 acaaggatcc gacttttaag atcaacaacc tggaaaggat cattaactgt gaatgatcgg
     2821 tgatcctgga ccgtataagc tgggatcaga atgaggggtt atacacaact caaaaactga
     2881 acaaccgttg ttctttggat aactaccggt tgatccaagc ttcctgacag agttatccac
     2941 agtagatcgc acgatctgta tacttatttg agtaaattaa tccacgatcc cagccattct
     3001 tctgccggat cttccggaat gtcgtgatca agaatgttga tcttcagtgt ttcgcccgtc
     3061 tgttttgcac cggaattttt gagttcagcc tcgagtttat caatagcccc acaaaaggtg
     3121 tcatattcac gactaccaat accgattgcg ccaaagcgga ccgcagaaag atcgggcttc
     3181 tgttcctgca atgcttcata gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg
     3241 gtggagctga taaccagcca gatccccgag gcagataaat cttccaacag cgggccgtgc
     3301 agcgtttcgg tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc tgctacatat
     3361 tcggcaccgc cgagggtgct gccgctgata agagtgatat ctgccataaa ccgccacctt
     3421 tattaagagt ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg
     3481 ggattaaaaa agccgatcag ggcttgatgg tacgcatgat cgggttctgc aggacgatca
     3541 gtgtctcggt ggactgaatt tcatcaattg tttggatctt gttgataagt acatgctgga
     3601 gagcatcgat cgaacggcac atcactttta taaagatgct gtagtggccg gtggtgtagt
     3661 acgcctcggt cacttcatcg aggctttcca gctttgccag cgcggaagga tagtctttgg
     3721 cgctctttaa tataatgccg ataaagcagc ctacgtcata accgagctgc ttcgggctga
     3781 catcaatacg cgccccggta atgatccccg cctgcttcat tttctctact cgaacgtgaa
     3841 tcgtccccgg actgacgcca aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc
     3901 ccattaatgc ttccaggatg ccacggtcca gattgtcgat tagataattt tccataggat
     3961 tttcttatgc agattgatga ttcattctat tttagccttc ttttttaatg aatcaaaagt
     4021 gtgtgaggct ttttattgaa tgattattgc atgtgtatcg gtttttgttg cttaatcata
     4081 agcaacagga cgcaggagta taaaaaatga aaaccgctta cattgccaaa caacgtcaaa
     4141 ttagcttcgt gaaatctcac ttttctcgtc aactggaaga acgtctgggg ctgatcgaag
     4201 tccaggcgcc gattcttagc cgtgtggggg atggcacgca ggataacttg tcgggctgtg
     4261 aaaaagcggt gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt
     4321 cactggcgaa gtggaaacgt cagaccttag ggcaacacga cttcagcgcg ggcgaagggc
     4381 tgtacacgca catgaaagcc cttcgccccg atgaagaccg tctttctccg ttgcactcgg
     4441 tctatgtaga ccagtgggac tgggaacgcg taatgggcga cggtgagcgt caattctcga
     4501 ctctgaaaag cacggtagag gcgatctggg cgggaattaa agcaaccgaa gctgcggtta
     4561 gcgaagagtt tggcctagca ccgttcctgc cggatcagat ccacttcgta cacagccagg
     4621 agttactgtc tcgttatccg gatctcgatg ccaaagggcg tgagcgggcg attgcgaaag
     4681 atcttggcgc ggtattcctc gtcgggattg gcggcaagtt gagtgatggt catcgtcacg
     4741 acgtgcgcgc accggattat gatgactgga gcaccccgtc agagctgggc catgcgggcc
     4801 tgaacggcga tattctggtg tggaacccgg tactggaaga tgcgtttgag ctttcttcca
     4861 tggggatccg cgtggatgcc gacacgctga agcatcagct ggcgctgacc ggtgacgaag
     4921 atcgcctgga gctggagtgg catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg
     4981 gcggcggtat cggccagtct cgtttgacca tgctgctgct gcaactgccg catatcggcc
     5041 aggttcagtg tggagtatgg ccagctgctg ttcgcgagag cgtcccttct ctgctgtaat
     5101 aatttatcgc cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat
     5161 atgatcgaaa atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa
     5221 gcgatgctga tgtacccgct gcagctcttt cactttactc gtcacgtcgt caggcaaccg
     5281 ctgggcgata aaatcagaaa tcaccaccgc atcggcatca aaccattccc tgctttgcaa
     5341 gcgttccata atggcgcgaa aacaactggc aagatcggtg ccgccacgaa actgctggct
     5401 taaaaaacgg attgcttgtt cgatgccttg tgggcctgaa agctcataac ggacgatctc
     5461 ggtggaaaat agcataatat aacagcgccg gttttcagcg agagcaatgc gcatcaaagc
     5521 caggcagaac gctttcgcac actgttcatt aaagccgccc attgagccgg aagtatccac
     5581 gcagacaata aacggtccgc gtggctgttc gtcgtaatct ttatgcacca ccgggcgttc
     5641 gatcactttt tcacgccacg actcaccgtg caggcgatag gtgagcaact gtttttccac
     5701 cagccgacgg taaaactcat actccagttc cgttatccct agtgtcgcca gttctggcgg
     5761 cagaagacgt aaaatatcat cgctttgttg cagaccatca acctgctcag gaaccgtcgc
     5821 cggttcgcgc accatggtgc ggaaggtttc catctgcgca tcgttacgcg gtattgattt
     5881 ggcttcccga gaacgcccca gctgctctgc caggcgtttc agttccggct gttcgttaag
     5941 aaattcaccg tatttcacaa tcaactgata gtcgccacgt ttaagctgac tggcgctcat
     6001 atcccacaga cgaccagctg cggtattgtt atctgcgaga atcggttcaa gttgtccgct
     6061 cagcgtcatg cgttcctgaa cttcactcaa cagttgttcg cgttcttctt ctaatagctg
     6121 ttgatttaac gtcgttgctt gcacgatcag acttaaacgc cagcgctgga gaaaaagcgt
     6181 gtgtaacgct gaagtgatcg tgctgttagt atcaaccaac tgccgggctt gttctgccca
     6241 tggggaattc agacgatgca gtaagtccat gatctgtggt agctgcacaa taaactgcgg
     6301 cgtggagagg agctggcttt gctgatagca catcacctct tcggtgagtt ccggcgggac
     6361 tcgggcatct ttcagccgac tgcgcagcgc ctcacgccag cggggaacat catcagtgat
     6421 tgccgccttc agtcgtggga atttttcaaa gaagaccgcc agctgcggtg aggccagcag
     6481 cgcgatgatc atctcttcga tcaatccctc ttcgctgacg gccagcatca cattaagcgt
     6541 atccagcgtt agcattgttg tgcctggcga atctgtgcgc cgacatcctg taggctggct
     6601 tcaatgcgac ctaaccagtc gccagggata aacagatatc tttgctgttc gctgaataaa
     6661 gcgtgttgct tgcgccagtc gctttcaagc tcttccagtt gttgttttat ttcacctggc
     6721 agaccttcag ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact tacatcgcgt
     6781 acaacaagat gttgggcgct atccacttcc agattcaatt tctgggcaaa gccgataccg
     6841 ttcagtttgc cgcgaatttc accaccttta ctcagccact gttccagcgc gttacgctca
     6901 aaggagatat gaaccacttc catatcatgc aattttaacg gtttttgcag cagcagagtc
     6961 agagtggaag cagtaacgtt aacaggaagt tgatactgct gacgacggct gaaaatgccg
     7021 cccagacgaa ttaccgttaa ggctgtttta tcgctttgtt gctgctggag ttgcaggtga
     7081 cgttgcacaa tcgcaccaag gcgagtcaac atcccttgct gttgccaggc gtgaccggtc
     7141 atcaatacat caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa
     7201 tctttcagca aaatgagatc taccggggca acagcactgc gaccgctaaa aaaggcgctg
     7261 gcctgcaata agcggatcgc ttttttccag cgacgatccg agacataagg cgcatccggt
     7321 aatttatcca gttgctggcg cagcataaaa atcagctcaa atacatgatc gggcagcgta
     7381 atttcaccaa tctctttctg ccagcgttca tattcttcat ctgtgacctg caaggcatca
     7441 ggaaccggat tgtcgttttc atcctgttga ctggtcagca tggagcggaa attcgcttta
     7501 tcctgcactt tatctaacca cagacgaatc agcatgcggt catataacgc ttccagactg
     7561 ctgtctgctt ccggcagctc gttggaggcc gccaccagca ggcgcatcgg gattttttct
     7621 acgtgtgcac cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt
     7681 gccggacccg ctttccagat ctcatccaga aagacaattt ctgcttccgg caggtaaccg
     7741 ctggttaaac gttcatagcg cccttcatct tttagcgcct gaatagaaag gggaccaaaa
     7801 acttcttccg gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc attctgaaag
     7861 gcgaatttta agcgccgggc gatcaaactt ttggcaatac ctggcgggcc aaggaggaac
     7921 acactttcac cacttaatgc cgctaataaa cacaagcgga tggcgtggct acgttcataa
     7981 agcccctttt ccaacgaact gctcaggcgg gaaattcttt ccgctaataa atgagggtga
     8041 gccataatga agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt
     8101 tcttgctggt ggctaaaata gtctcaaagg gggggtattt ttctttgagc caggttaatg
     8161 tggccgcatt taggagtacg attttgccgt taatcgtgca tactgtgcgc ttttttgtgg
     8221 gccaagggac taagcacaca tttcatattt caacgaaaga ctagtctatg agcactgata
     8281 ataagcaatc attgcccgcg attaccctcg cggcgattgg agttgtctac ggcgatattg
     8341 gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt tttggcgttg
     8401 aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc tttgtggttt
     8461 ccattaaata tctcaccttc gtgatgcggg cagataacgc tggtgaaggg gggatcctga
     8521 cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg ctggtgatta
     8581 tggggctaat cggcggcagc tttttctatg gtgaagtcgt cattaccccc gcaatttcgg
     8641 tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc tggatagttc
     8701 ccctctcaat tatcgttctc actttattat ttatgattca aaaacatggc acggctatgg
     8761 ttggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca ggactggggt
     8821 tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg tgggcggtgc
     8881 atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg gtgctgtcga
     8941 ttacgggggt cgaggcgctg tatgctgata tgggacactt tggtaagttc cctattcgtc
     9001 tggcgtggtt cactgtcgta ttaccttcct taactcttaa ttacttcggc cagggagcgc
     9061 tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca ccggactggg
     9121 cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg caggcggtta
     9181 tttctggcgt cttctcattg acgcgtcagg cggtacgtct ggggtatttg tcgccgatgc
     9241 gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt gtgaactgga
     9301 tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc aacctggcgg
     9361 cggcgtacgg gattgcggtg accggaacca tggtgctgac gtctattctc tcgactaccg
     9421 tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg attgctttcc
     9481 tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc ggcggctggt
     9541 tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag agcgagcgtt
     9601 tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg attgcttcgc
     9661 tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg cgtgcaatca
     9721 acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg catgagcggg
     9781 tgattctgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc cgtcgggtac
     9841 agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt tggcgagaaa
     9901 cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt tgccggatga
     9961 tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc ccgtggtatt
    10021 tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgg gcaccggatc
    10081 aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa atctaacgcc
    10141 agacgcctcc tttctccata agggggcgtt tttgttttca tggttaatca ccatgtaaaa
    10201 cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact cagtcaggat
    10261 taaactgcgg gtcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa aggcaccgtt
    10321 cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac gctggtggtg
    10381 tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc attaacccag
    10441 ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca ggtcgaggct
    10501 gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac gttgctcact
    10561 caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta caccacgcat
    10621 gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg agaatgttct
    10681 ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca tggaagcatt
    10741 acttcagctt aaaggcatcg ataaagcctt cccgggcgta aaagccctct cgggcgctgc
    10801 gttaaatgtc tatccgggcc gcgtgatggc gctggtgggc gaaaacggcg cgggtaaatc
    10861 caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc ttttatggct
    10921 ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga ttgggattat
    10981 ccatcaggaa ctgaacctga tcccgcagtt aaccattgcc gaaaacattt tcctcggtcg
    11041 tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgccg aagcggataa
    11101 attgctggct aaacttaacc tgcgctttaa aagcgacaag ctggtgggcg atctttccat
    11161 cggtgaccag caaatggttg aaatcgccaa agtgctgagc tttgagtcga aagtcatcat
    11221 tatggatgaa ccgaccgatg cgctgaccga taccgaaacc gaatccctgt tccgcgtcat
    11281 ccgcgagctg aaatcgcaag gccgcggtat tgtctatatc tcccaccgca tgaaagaaat
    11341 cttcgagatt tgcgatgacg ttaccgtttt tcgtgatggg caatttattg ctgagcgcga
    11401 agtggcatca ctgaccgaag attcgctgat tgagatgatg gtgggtcgca agctggaaga
    11461 tcaatatccg cacctggaca aagcgccggg agatatccgc ctgaaagtcg ataatctctg
    11521 cggacctggc gttaacgatg tctcttttac tttacgcaaa ggcgaaattc ttggcgtctc
    11581 tggtttgatg ggcgcaggtc gtaccgaact gatgaaagtg ctctacggcg cactaccgcg
    11641 caccagcggt tacgtcaccc tggatgggca tgaagtcgtt acccgttcac cgcaggatgg
    11701 cctggcaaac ggcattgtgt atatctccga agaccgtaaa cgtgacggtt tagtgctggg
    11761 catgtccgta aaagagaata tgtcgctgac cgcgctgcgc tacttcagcc gcgctggcgg
    11821 cagtttgaag catgccgatg aacagcaggc agtgagtgat tttattcgcc tgttcaatgt
    11881 gaaaacgccg tcgatggaac aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt
    11941 ggcgattgcc cgcggtctga tgacacggcc caaagtattg atccttgatg agcctacccg
    12001 tggcgtagat gtcggcgcga aaaaagagat ctatcaactg attaaccagt tcaaagccga
    12061 tggcttgagc atcattctgg tgtcatcgga gatgccagaa gtattaggca tgagcgatcg
    12121 catcatcgtc atgcatgaag ggcatctcag cggggaattt actcgtgagc aggccaccca
    12181 ggaagtgtta atggctgccg ctgtgggcaa gcttaatcgc gtgaatcagg agtaaaaaaa
    12241 tgacaaccca gactgtctct ggtcgccgtt atttcacgaa agcgtggctg atggagcaga
    12301 aatcgcttat cgctctgctg gtgctgatcg cgattgtctc gacgttaagc ccgaactttt
    12361 tcaccatcaa taacttattc aatattctcc agcaaacctc agtgaacgcc attatggcgg
    12421 tcgggatgac gctggtgatc ctgacgtcgg gcatcgactt gtcggtaggt tctctgttgg
    12481 cgctaaccgg cgcagttgct gcatctatcg tcggcattga agtcaatgcg ctggtggctg
    12541 tcgctgccgc tctcgcgtta ggtgccgcaa ttggtgcggt aaccggagtg attgtagcga
    12601 agggtcgcgt ccaggcgttt atcgctacgc tggttatgat gcttttactg cgcggcgtga
    12661 ccatggttta taccaacggt agcccagtga ataccggctt tactgagaac gccgatctgt
    12721 ttggctggtt tggtattggt cgtccgctgg gcgtaccgac gccagtctgg atcatgggga
    12781 ttgtcttcct cgcggcctgg tacatgctgc atcacacgcg tctggggcgt tacatctacg
    12841 cgctgggcgg caacgaagcg gcaacgcgtc tttctggtat caacgtcaat aaaatcaaaa
    12901 tcatcgtcta ttctctttgt ggtctgctgg catcgctggc cgggatcatt gaagtggcgc
    12961 gtctctcctc cgcacaaccc acggcgggga ctggctatga gctggatgct attgctgcgg
    13021 tggttctggg cggtacgagt ctggcgggcg gaaaaggtcg cattgttggg acgttgatcg
    13081 gcgcattaat tcttggcttc cttaataatg gattgaattt gttaggtgtt tcctcctatt
    13141 accagatgat cgtcaaagcg gtggtgattt tgctggcggt gctggtagac aacaaaaagc
    13201 agtaataacg actacaggac atcttgaata tgaacatgaa aaaactggct accctggttt
    13261 ccgctgttgc gctaagcgcc accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc
    13321 tggtggtctc cacgcttaac aacccgttct ttgtatcgct gaaagatggc gcgcagaaag
    13381 aggcggataa acttggctat aacctggtgg tgctggactc ccagaacaac ccggcgaaag
    13441 agctggcgaa cgtgcaggac ttaaccgttc gcggcacaaa aattctgctg attaacccga
    13501 ccgactccga cgcagtgggt aatgctgtga agatggctaa ccaggcgaac atcccggtta
    13561 tcactcttga ccgccaggca acgaaaggtg aagtggtgag ccacattgca tctgataacg
    13621 tactgggcgg caaaatcgcc ggtgattaca tcgcgaagaa agcgggtgaa ggtgccaaag
    13681 ttatcgagct gcaaggcatt gctggtacat ccgcagcccg tgaacgtggc gaaggcttcc
    13741 agcaggccgt tgctgctcac aagtttaatg ttcttgccag ccagccagca gattttgatc
    13801 gcaccaaagg tttgaacgta atgcagaacc tgttgaccgc tcatccggat gttcaggctg
    13861 tattcgcgca gaatgatgaa atggcgctgg gcgcgctgcg cgcactgcaa actgccggta
    13921 aatcggatgt gatggtcgtc ggatttgacg gtacaccgga tggcgaaaaa gcggttaatg
    13981 atggcaaact agcagcgact atcgctcagc tacccgatca gattggcgcg aaaggcgttg
    14041 aaaccgcaga taaagtgctg aaaggcgaga aagttcaggc taagtatccg gttgatctaa
    14101 aactggttgt taagcagtag ttttaatcag gttgtatgac ctgatggtga cataaatacg
    14161 tcatcgacag atgaacgtgt aatataaaga aaagcagggc acgcgccacc ctaacccggt
    14221 ggcgcatttt atggacatcc cgaatatgca aaacgcaggc agcctcgttg ttcttggcag
    14281 cattaatgct gaccatatcc ttaatcttca ctcttttcct actccaggcg aaaccgtaac
    14341 cggtaaccac tatcaagttg catttggcgg caaaggcgca aatcaggctg tggctgctgg
    14401 gcgtagcggt gcgaatatcg cgtttattgc ctgcactggc gatgacagta ttggcgagag
    14461 tgttcgccag cagctcgcca ctgataacat cgatattact ccggtcagcg tgatcaaagg
    14521 cgaatcaaca ggtgtggcgc tgatttttgt taatggcgaa ggtgagaatg tcattggtat
    14581 tcatgccggc gctaatgctg ccctttcccc ggtgctggtg gaagcgcaac gtgagcgtat
    14641 tgccaacgcg tcagcattat taatgcagct ggaatcacca ctcgaaagtg tgatggcagc
    14701 ggcgaaaatc gcccatcaaa ataagactat cgttgcgctt aacccggctc cggctcgcga
    14761 acttcctgac gaactgctgg cgctggtgga cattattacg ccaaacgaaa cggaagcaga
    14821 aaagctcacc ggtattcgtg ttgaaaatga tgaagatgca gcgaaggcgg cgcaggtact
    14881 gcatgaaaaa ggtatccgta ctgtactgat tactttagga agtcgtggtg tatgggctag
    14941 cgtgaatggc gaaggtcagc gtgttcctgg attccgggtg caggctgtcg ataccattgc
    15001 agccggagat acctttaacg gcgcgttaat cacggcgttg ctggaagaaa aaccgttgcc
    15061 agaggctatt cgttttgctc atgccgccgc tgcgattgcc gtaacgcgta aaggcgcaca
    15121 accttccgta ccgtggcgtg aagagatcga cgcattttta gacaggcaga ggtgacgctt
    15181 ggctacaatg aaagatgttg cccgcctggc gggcgtttct acctcaacag tttctcacgt
    15241 tatcaataaa gatcgcttcg tcagtgaagc gattaccgcc aaagttgaag cggcgattaa
    15301 agaactcaat tacgcgccat cagctctggc gcgtagcctc aaactcaatc aaacacatac
    15361 cattggcatg ttgatcactg ccagtactaa tcctttctat tcagaactgg tgcgcggcgt
    15421 tgaacgcagc tgcttcgaac gcggttatag tcttgtcctt tgcaataccg aaggcgatga
    15481 acagcggatg aatcgcaatc tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt
    15541 actgtgcacc gaaacgcatc agccttcgcg tgaaatcatg cagcgttatc cgacagtgcc
    15601 taccgtgatg atggactggg ctccgttcga tggcgacagc gatcttattc aggataactc
    15661 gttgctgggc ggagacttag caacgcaata tctgatcgat aaaggtcata cccgtatcgc
    15721 ctgtattacc ggtccgctgg ataaaactcc ggcgcgcctg cggttggaag gttatcgggc
    15781 ggcgatgaaa cgtgcgggtc tcaacattcc tgatggctat gaagtcactg gtgattttga
    15841 atttaacggc gggtttgacg ccatgcgcca actgttatca catccgctgc gtcctcaggc
    15901 cgtctttacc ggaaatgacg ctatggctgt tggcgtttac caggcgttat atcaggcaga
    15961 gttacaggtt ccgcaggata tcgcggtgat tggctatgac gatatcgaac tggcaagctt
    16021 tatgacgcca ccattaacca ctatccatca accgaaagat gaactggggg agctggcgat
    16081 tgatgtactc atccatcgga taacccagcc gacccttcag caacaacgat tacaacttac
    16141 tccgattctt atggaacgcg gttcggctta gatttacgct gtcttttgat caaattatta
    16201 ccatcggttg ttttcagaag catgaacatt gctgctgaag caacagtaat aatgcccatc
    16261 gtgataaacg tatagtggaa ttgttcgaca gtcgttgtgc cttccatccc ttcataaacg
    16321 cgaaggacgg ccgcacttac agcaacgcct aaactgatag acagttgctg cgtgaccgcc
    16381 agaacactgt taccgctgct ggcgttgtca tcggtcagat cggcaagtgt gatggtattc
    16441 atcgcggtaa actgcgtcga catagccatc cctaatataa acaacggcaa gatcagcatc
    16501 catatcgcca ttgccggtga ttgcaaagag aactgagcga tcattagccc aataatcacc
    16561 gtgatcccca ctaatgtatg gcgatagccc agacgacgta agacttgggt aaccatcgat
    16621 tttgcaataa tggaacctaa cgctgtcggt gccatcatac agccggcaat aaacgcctga
    16681 taaccaaatc ctacctgtaa catcaatggc ataaggaacg gtacacagcc ggtccccaga
    16741 cgggttgcaa tattgcctac gataccgatc gagaaagtgc gggttttaaa taaatctaat
    16801 gaaattaatg ggtttggcgt gcgtcgtgca tggagaatat agagaagcag taacccgatg
    16861 ctggtgacaa ttaccgtcaa ggcaatccag ctggcgacaa tcttttcccc gaatagctct
    16921 attccgcttg agaagagaac aaggctgagg ccaaacagca aaaagccagt gatatcgaat
    16981 ctgcgtcgtg cggtggtgaa attgggcata tgtttgcgcg cgtaaagaag gcccgcaata
    17041 cctatgggga tattgattaa aaatatccag tgccaggttg cccaggtgac cagcacgccg
    17101 ccaagaacgg ggcctaaaat tggccccacc agacccggca tggcgacaaa attcaatact
    17161 ggaagaagtt cattacgagg ataagcgcgc agtaaggcca gccgagcaac aggcatcatc
    17221 attgcgccgc ctatcccctg aataacccgg aagacaacca gctgtggtag cgagttggaa
    17281 agtgcgcagg ccagagaacc caacgtgaac agactcacgg caagggtaaa aatgcgacgc
    17341 gtaccgaagc gatcggctag ccatccgctt accggaataa gcatcgccac cgtcagcgta
    17401 taactgatga tggctgattg catcgcgaga ggagaacgat taaggctatg agcgattgcg
    17461 ggtaaggcgg tattcagaat agtggcatca agtgcctgca tgaagaaggc catcgccgcg
    17521 atccacggca aacccgccat actgcgcttc tttttatcgc tcattcaatg tcctgttatc
    17581 gggttatcac ttatcaggtg agcgtagcag cgcctgacaa gctttaaatg ccgcgtcgcc
    17641 atcgctttgg ataatcgcat cgacaatcgc ctgatgcaga tccagcttta tcactgtgtc
    17701 gctggtaatt gacgtgaagt aagtgtgata aaccgaatgg aatagcgagg cgaatgaggt
    17761 caaaaacgga ttggcgctca tttcatagat atgctcatgc caggccatat cgacttcgat
    17821 ccagcgttca cggcgaaagt tctcttttaa tgccgccatt tcggccatta acgtattgag
    17881 atgcgctttc tgttctgcgg tgccaaccgt tgctgccagt aggcaggctt gcggctccag
    17941 acagatacgc ataaccagaa agtgatcgat gacctgatga aagttctctt ctgtcatcca
    18001 ccaggtaagc aattcctgat caagaaaatt ccagttcgat tgtggcatta cccgggtacc
    18061 aattcgcggt cgcggtaaaa ccatcccttt tgccgttaac gttttgaccg cttcgcgtac
    18121 cgctgtacga ctcactccaa attgctcgcc cagctcaatc tcaccgggca aaatggtgcc
    18181 gggttcatat tcacctttta agatccgttg cgccagcttc tcagccagaa catacgaaag
    18241 gtttttctgt gcagctaact gttgtgcgct taaaggcatt actaatcttc ctttttcttt
    18301 ttattcctcc ttagtatgcc accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc
    18361 ttgttttatg gcacattaac gggggttttg ctgaaaaaat gcgcggtcag aaaattattt
    18421 taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac
    18481 aacggcaaac acgccgccgg gtcagcgggg tt
//
LOCUS       DAZUGK010000086        18143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZUGK010000086 DAZUGK010000000
VERSION     DAZUGK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.086"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2242
                     /locus_tag="ACHY5W_002555"
     CDS             <1..2242
                     /locus_tag="ACHY5W_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFS4447436.1"
                     /translation="FFMTGNACATEWISSEDLITSDFHLMTADERNVVKAATDDSMEA
                     AYMLKDNIRWYYHNGDLSLPANFSNQNKLVVNGNLTISGDYDDYLSGNGHLIVLGNVI
                     VDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFEVIKAEF
                     YINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADNIVQGLP
                     LFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVMLQLLQH
                     PDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADVNKKLMSVPVESVQ
                     LEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELINNEDPELRER
                     ITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTEDIERIAEQMYLDN
                     KENKNIVKALLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQHDVPTVWDE
                     LARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEEMLNNAIDL
                     LVDLPAEYRYRMRNQLFDNKDLPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSERG
                     LHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLTE
                     SQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDQPDLSQLRDLVKTGATRKIRSEARH
                     RLEEKQ"
     gene            2239..2694
                     /locus_tag="ACHY5W_002556"
     CDS             2239..2694
                     /locus_tag="ACHY5W_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447437.1"
                     /translation="MTQRPWSKLQRENYDLLTPTINLHIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICPISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRFDQLRRKTHNIAALKIIARRSE"
     gene            complement(2772..3683)
                     /gene="rdgC"
                     /locus_tag="ACHY5W_002557"
     CDS             complement(2772..3683)
                     /gene="rdgC"
                     /locus_tag="ACHY5W_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFS4447438.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3808..4716
                     /gene="mak"
                     /locus_tag="ACHY5W_002558"
     CDS             3808..4716
                     /gene="mak"
                     /locus_tag="ACHY5W_002558"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFS4447439.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4859..6043)
                     /gene="araJ"
                     /locus_tag="ACHY5W_002559"
     CDS             complement(4859..6043)
                     /gene="araJ"
                     /locus_tag="ACHY5W_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFS4447440.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMILTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(6169..9312)
                     /gene="sbcC"
                     /locus_tag="ACHY5W_002560"
     CDS             complement(6169..9312)
                     /gene="sbcC"
                     /locus_tag="ACHY5W_002560"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFS4447441.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKLLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTVPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(9309..10511)
                     /gene="sbcD"
                     /locus_tag="ACHY5W_002561"
     CDS             complement(9309..10511)
                     /gene="sbcD"
                     /locus_tag="ACHY5W_002561"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFS4447442.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFTTTLH
                     TLAGESEA"
     gene            10701..11390
                     /gene="phoB"
                     /locus_tag="ACHY5W_002562"
     CDS             10701..11390
                     /gene="phoB"
                     /locus_tag="ACHY5W_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFS4447443.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11448..12743
                     /gene="phoR"
                     /locus_tag="ACHY5W_002563"
     CDS             11448..12743
                     /gene="phoR"
                     /locus_tag="ACHY5W_002563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFS4447444.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            13150..14469
                     /gene="brnQ"
                     /locus_tag="ACHY5W_002564"
     CDS             13150..14469
                     /gene="brnQ"
                     /locus_tag="ACHY5W_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFS4447445.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14545..15918
                     /gene="proY"
                     /locus_tag="ACHY5W_002565"
     CDS             14545..15918
                     /gene="proY"
                     /locus_tag="ACHY5W_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFS4447446.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16074..17891
                     /gene="malZ"
                     /locus_tag="ACHY5W_002566"
     CDS             16074..17891
                     /gene="malZ"
                     /locus_tag="ACHY5W_002566"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFS4447447.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGSQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         4203 a         4465 c         4836 g         4639 t
ORIGIN      
        1 attttttatg actggcaatg catgtgccac agagtggatc agtagcgagg accttatcac
       61 ctctgacttt catcttatga cggcagatga gcgcaacgtg gttaaagcgg caaccgacga
      121 tagtatggaa gctgcttata tgctaaaaga taatatccgc tggtattatc ataatggtga
      181 cctatcatta cccgctaact tcagcaatca aaataaactg gtggtgaacg gtaatttaac
      241 gatttccggc gattatgatg attatctgtc aggtaatggt catctgattg tattaggtaa
      301 tgttattgtc gataacttta ttaatcatga tttcgcgtat gtcaaaggac agatgacagc
      361 gaaaggttta gtttatgctg attacaatga tcataacttt gaagtgatga agggaattag
      421 tgcgcgaggc attattgttt ccgataaagc aacgcaattt gaagtcatta aagctgaatt
      481 ttatatcaat gaagatggaa gtggtgaagg atataactgg gatgagaata ttcaaaaagc
      541 ctattcactg gtgactgccg atctttatga tcacactgaa atcgaaaccg ataacatttc
      601 caatgcgtat cctgactatg atagcgtcgc agacaacatc gttcagggtt taccgctttt
      661 tcgagataaa gccgctcctg aaattaatga gaaattaaaa tggattgaaa cggggaaact
      721 cgataatttc ccagcgaata aaataaagca tcaagatcca ctggtggcac gttttttgac
      781 tcatacagag agtttatcac ccgccgttat gctgcaactg ttacagcacc ccgatgacca
      841 gacgcgtgaa tcgatggcgc agtcctggcc cgctcaacag atgcatttgc ttaccgatga
      901 attaattaaa gatgaggctg ttgccagagg gctggttaaa aacagtaata tttccgccga
      961 tgtgaataaa aaattaatgt ctgtacccgt cgagagcgta cagctggagc aggccagaca
     1021 ggataatctt tctccagata ttgttgcgtc gttaagtcat agcccctttc tcagtgtacg
     1081 caagacatta ctcagccatt acgattacgc ctggctggtt ccgactgcag tggccgatga
     1141 gttgataaat aacgaagacc cggagttacg tgaaagaatt accggagcag atttaaccgc
     1201 acaacaagca gtgatgttga gtaaagataa atcactgaaa gttcgtgaag cattagcccg
     1261 gacgcttact gaattaaaaa ttacccaact gtcagcaacc ctgcgtacag aggatattga
     1321 acgtatcgca gaacaaatgt atctggataa taaagaaaat aaaaatatcg taaaagcgtt
     1381 attgatagcg ttgccggaga tgcgtcaact gtctctggca aaagaagatg tgcacaattt
     1441 gcgcgaaggt gcgcgttatc tgacatcgaa ggacgtgatt agttatctat tgactcagca
     1501 tgatgttccc accgtctggg atgagctggc acgcgataaa ttattgccgc tggaatataa
     1561 gaaacagtta tggcaacgca cattaaattt aatgatgagc aaacggcaag aagatcaaga
     1621 gcaagcctac gaagtacagc tggcgctgat tgataatggt gttgttgacg aagagatgtt
     1681 aaataatgcc attgatttac tggttgattt acccgctgaa taccgctatc gaatgcgtaa
     1741 ccaattattt gataataaag atttaccttc cggaattatc aacaaactcg atcaacagta
     1801 tcgcttcaac tctgactggg cgctggctgt tgtcagtatg aaaaatagca cccgtaggca
     1861 aagtgaaaga ggattacacc gctggaacca tgaggatagc gatatttttg cggaactggc
     1921 gacgataaaa gataaatcag atgatgaatg gtggcgtgca ttgctgcaaa gtcgcaatga
     1981 tcatttgcgc caaacggcat tacgcaacgc acataccccg gcgtcgttgt taacgacatt
     2041 gactgagtct caggatcggt cgctggctat taataatcca cagctggctg ccgatgtgaa
     2101 aacggtgtgg ctaaaagagg atccatcatt acttttattt gtcgatcaac ccgatctttc
     2161 gcagttacgg gatttagtga aaaccggggc aacgcggaaa attcgcagtg aagcgcgtca
     2221 ccggcttgag gaaaaacaat gacacaacgc ccctggagca agttacagcg ggagaactat
     2281 gacttactga cgccaacgat caacctgcac attcactgta cccgttatcc catgcgcagc
     2341 caaaatggtg gaagcaccga tttaccgcgt tattggatca cgttggataa aaacgtgatc
     2401 tgggattacc cgaaagactt tattgcaggt aacggtggag tgcgtaattt tcacggtgaa
     2461 acttgctggt atccttacct gacggatatt tgtccaattt ctgatttatt gcgtgaatat
     2521 attgacaccc caaaagcgga gctgttaaca aaacaattta ccagcgataa atgggggtta
     2581 gtgaatattc tgcgtgctgc tgacaggcga atagggatga gacgattcga tcagttacgg
     2641 cgtaaaacgc ataatattgc tgcgctaaaa attattgccc gccgtagcga ataattatat
     2701 gcctggtgtg gcttcgtacg ccggataaga cgcggcaggc gtcgcatccg gcattaaagg
     2761 aaaatcagca attaacgttg tgcttcacca cctaatcctt caatcaggtt ttgaattaac
     2821 gctgccagtt caccagtcat caggatgaaa tcggcatcaa aacgctgggc gaaatcttca
     2881 cggtcgatat cttcgttttg atcgcgcagc tcgtcgcaga acttcagacg cttgagcgaa
     2941 ccatcgtcgc acatcacaaa ctgaatgcgc tgctgccagt cgagcgccag tttagtcacc
     3001 acttttccgg cttcaatgtg attggtgatc tcttcgctgg tcagatcttg tttcttcgcg
     3061 cggatcactc cgccatcttc cagcaacgat ttcagctcgg cttcatcaag cagctggaag
     3121 ccctgtgccg cactaccgga acgtacccat tcggtcagcg tcagttcaat cgggttttcc
     3181 atgctcaatg gtacaaccgg taacgacccc aggcttttac gcagcagtgc cagcgtatct
     3241 tccgcttttt tggcactggc gcagtccacc ataatcaaac cattaaccgt gtcgatccac
     3301 atcattgtct ggctaaaacg gctgaaagca cgcggcagca gagagtgcag cacttcgtct
     3361 ttcagcgaat ctttttcggt tttcttcagc ttacgcgcct gttccgcttc cagtttggcg
     3421 attttcgctt ccagcgcctg tttaatcacc ggagacggga ggattttttc ttctttgcgt
     3481 gcgcagataa caatttgacc attggcaacg tgcgttaacg catcgctgtg cgatcccatc
     3541 ggaggaaccc agcccatctt cgccatgtcc tggctgccgc atggggtaaa tgccatcgag
     3601 gctagctgtt tttccatctc ttctgcacgc agcgaaatct cgcggctaag acggtaaacc
     3661 attaaatttt tgaaccacag catgataatt tccacggcct tgtcgttaaa tttagcgggc
     3721 atgataacga attgtcggcg gccttgcatt gccaatccgg ttgtccgtct ctacgctatt
     3781 gatattgaaa aaaataagga gagtaccgtg cgtataggta tcgatttagg cggcaccaaa
     3841 actgaagtga ttgcactggg cgatgcaggg gagcagttgt accgccatcg tctgcccacg
     3901 ccgcgtgatg attaccggca gactattgaa acgatcgcca cgttggttga tatggcggag
     3961 caggcgacag ggcagcgcgg aacggtaggt atgggcattc ctggctcaat ttcgccttac
     4021 accggtgtgg tgaagaatgc caattcaacc tggctcaacg gtcagccatt cgataaagac
     4081 ttaagcgcgc ggttgcagcg ggaagtgcgg ctggcaaatg acgccaactg tctggcggtt
     4141 tcagaagcag tagatggcgc ggcagcggga gcgcagacgg tatttgccgt gattatcggc
     4201 acgggatgcg gcgcgggcgt ggcattcaat gggcgggcgc atatcggcgg caatggcacg
     4261 gcaggtgagt ggggacacaa tccgctaccg tggatggacg aagacgaact gcgttatcgc
     4321 gaggaagtcc cttgttattg cggtaaacaa ggttgtattg aaacctttat ttcgggcacg
     4381 ggattcgcga cggattatcg tcgtttgagc ggacatgcgc tgaaaggcag tgaaattatc
     4441 cgcctggttg aagaaagcga tccggtagcg gaactggcat tgcgtcgcta cgagctgcgg
     4501 ctggcaaaat cgctggcaca tgtcgtgaat attctcgatc cggatgtgat tgtcctgggg
     4561 ggcgggatga gcaatgtaga ccgtttatat caaacggttc cgcagttgat taaacaattt
     4621 gtcttcggcg gcgaatgtga aacgccggtg cgtaaggcga agcacggtga ttccagcggc
     4681 gtacgcggcg ctgcgtggtt atggccacaa gagtaaaaaa cgtaggcaat tggcgcatca
     4741 tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa aatgtgccag aaccgcgtag
     4801 ggcggataag gcgttcacgc cgcatccggc aataagcaac gcgctccgtg actgcaatct
     4861 accccagagg tttcgccagc accggagtat ccgccgcttg ctggcgctta tagcgaccat
     4921 acagcagcaa tgacgacatc gcagcaaacg aaagcagggc ggcaggcagc gccacgtaat
     4981 tatatgccag ccccagcgtc agtatcatac ctccgcaata tgcgccgacg gcgctaccga
     5041 ggttaaacgc tatttgccca cctgcggcac ctaataactc tccgcctttg gcattttgca
     5101 gtaacaatat ttgcagaggt gctgaaaggg caaataatcc cgcgcaacaa ataaaagcaa
     5161 aaataagcga cgttgttttc atgccgccgc agaaaaagag catcagcagt gccagtacaa
     5221 tgataaagtc agtcactgct gcaatgcgca gtggtgaata acgtcctgaa atcctgccac
     5281 ttagcatatt tcccagcacc atccctagcc caactaacat cataataaag gtcatcgccg
     5341 tttccgaaaa accggaaata aacatcatgt atggctttac gtagctgaac caggcaaaca
     5401 cacctgcgtt gccaaacatg gtggcggcga aaattaacca cggggccggg ctgcgcaaaa
     5461 agtgaaattg ttcgcgcagc tttcctttcg cctcgtcgcg aatatccggc acccaaaaat
     5521 agaccgatgc catcaccgca atattaaaaa cagcgatcaa taaaaaggtg taacgccagc
     5581 taaattcctg acttaaatac gttcccagcg gaatgcccag caaattggcg actgtcatcc
     5641 cggaaaccat ccccgccacg gcggcggtga cttttccggg tttgataatt ttcgataaca
     5701 cgatcgcgcc gacgccaaaa aatgcgccat gcggaaagcc ggaaaccagg cgaccaatgg
     5761 caagcatcag gtaagacgaa gagagcgtga acatggcgtt accaatgacg cacaacgcca
     5821 ccagaaacaa caagatatgt ttgagtgagt agcggctgga aaagagtgcg atgattggcg
     5881 caccgaccac cacccccagc gcataatacg agatcatatg cccggcggca ggaatcgaaa
     5941 ttcctacgtt atgcgccagc tccgtgagca cgcccataat gccaaattcg gccatcccca
     6001 aaccaaacgt gcccagagcc aaagataaaa tgactttttt cataccacca tcacaaccac
     6061 cagaacggct actaatcacc gtagccaata aagtgatagc ttgcaggata acgaccagcg
     6121 aggccaagag ataattcgcc cctctgtatt cattatcctg ctgaatagtt atttcactgc
     6181 aaacgcactt tccagtttgc tgtagcccag gccgttgatc tttttcactt tgatctgcac
     6241 cggaatacgc tctttcatcg cttctacgtg gctaatcaca ccgatggttt tgccactggc
     6301 gttcagggca tccagcgcat caagggcggt atccagcgtt tcgctatcca gcgtgccaaa
     6361 accttcatca aggaacagcg agtcaatacg cgttttatgg ctgaccagat ctgaaagcgc
     6421 cagcgccagc gccagactga cgaggaaact ttcgccgcca gaaagggtac gggtgtcacg
     6481 taccgcatct gcctgccagg tatcaacaac ctcgacttcc agcgcctcgc tggctttgcg
     6541 ctgtaacaga tagcgcccgt gcagtcgggt aagttgctga ttagcgagat ggactaaatt
     6601 atccagcgtc agcccctggg caaacttgcg gaatttatcg ccctctttgg aacctattag
     6661 cgaattcaga tatccccagt cctcaacctg ctgcgtcatt tgagcaattt gctgcattaa
     6721 ggtttgttgt tgctgacggt tatctgcatc ctgcttcagc tgctggcgaa tctcgccttg
     6781 actcgtggtg ttttcacgca acttttggtg agtttgcgct aactcttgct gaatctgctc
     6841 caccgtcaca gtgagagcca acccgtcagg tcggtgttgc tgatgctgtg ccagcgtttc
     6901 tgctgtctga gtgaccagcg tttgcgcctg acggcgctgg ttttccagat tctgcttgag
     6961 ctgttccagc tgcgttagtg tttgttcatc cattagcgcc gcaaggaacg cctgctgatc
     7021 gtcaaagacg ctggcctgta gcgcggtgtc aaactgcgcc tgggcttttt gcagactttg
     7081 cgccgccaga acgtcctgtt gctgtaacgt ctgctgctgg ctgtgtaatg cgagacattg
     7141 ttcatgtacc tgccgccagt tttccagtac cacagtttct tcgcagtgcg ggagttcatc
     7201 actttgcggc aacgtttcca gaatcggcgt cagctgctga atacggtttt gcagcgcagt
     7261 taattcgttc tggcgatgct gccagctctg cgcttcttgt tgacgtgtcg ccaaccagct
     7321 ctcttcttca tcttcctgtg gcactgtcag tgcataaccc gccaatgccg ttaagagttg
     7381 ctgctggcgt tgttcaattt gctgttgata ctggataatt tgctgattat gcgcggcaat
     7441 ctgcccttgt aattcatgcc gttggctgag taaccgcagc tggcgttcgt gctcatcttg
     7501 tgcatccagc cacggttgaa tatcgtcctg tggctgcaac gtgatattga ggctggccgt
     7561 gacggcttgc cattgttgag taagtgcttg ctcatcttgt cggaggcttt gcgcttcgtt
     7621 ttcatcacgc tgaagctgct ttgttaaggc gtccagttgc ccacgtagcg tcgcaccttc
     7681 ttcaccgagc tttttaactt cgttttccag cgccagtaat cgagactgat taacaccagg
     7741 ctccagcgcc tgatacgcct cgaccgccgg gtggctggtg gaaccacaaa gtgggcaagg
     7801 ctgacccgcc tgtaactgtg cacgttgagc ttccagcgtt ttgatgcgcg cttcctgctc
     7861 gcaaatggtt ttgacatcgg caagttgctg cgtcttttct ttataacgct ggcgcatttc
     7921 gttaagtgcg gcattacgtt gcgtctgttc cagagtgaca ttctggatag tgaccattaa
     7981 ctgcgccaga cgtttttgtt ggggaacaat ctgcccatgc agcgcgacca ggcgctgacg
     8041 cagtgggcgt tgctcggcat gttgcgccag ggcgctagca acttcatcgg cggttaacgt
     8101 caacgtgatc gccgcaagcg tattaagttt ttgctcagca tgggttaact gttgctgcca
     8161 ttgccgcaga tgctcgcgat cgctggtttg ttgggagaac tgcgcacgcc aacccgccag
     8221 ttcgttgttc cactgacgga agcggtcgtg ttcctgtaac caggtattca ggctttgttg
     8281 ctgctgctgt aattctgctg actgcttcgc cgcgtggtgg cgaatgctcg cgcgaagcgc
     8341 cattgtgctc tgtaagcgag tatttacttc ttcaatctgc tggcgagtat gcgccagcgc
     8401 cgcgctgtgt tctgcgatgc gttcccagtg cggacgaaga tttcgtgccg gttgtgccag
     8461 actgagcgcc gccagttgag gttgcgcttt ttcttcttcg gctaacgcct gttgcaaggc
     8521 ctgctgacgg cggctggctt cttgctgcaa ttcgtccaga cgcgttaacc agtttagtga
     8581 ttgttgttct tgctgctgcg cggtaagtaa cagtttttct tcgtcagtaa gtacctgcaa
     8641 actcgctgtc agcgattgca cttgttctgg cgtgagcaac gcgacgccgc tggcctgtgc
     8701 ttgcagcttc tccagctcag tgcgggccga tttgtgctgc tcaaaaacca tcgccgagat
     8761 ttgcccgtag atttcagtgc cagttaactc ctcgagcaat tccgcgcgtt ctttgggttt
     8821 ggcattcagg aaggcagcaa attgcccctg cgaaagcagc atcgaacggg tgaagcgccc
     8881 gtaatccagc ccggttaacg tcgctgtcag ttccagctta tctttcactt tgtcggcgag
     8941 aattttgccg tcggcgcagc gcgccagctc tacgcgtggc acctgcaaat taccgtcggg
     9001 ttggttacgc gcccgattct ggctccagaa cgcgcgatac gcttcacctt tcacttcaaa
     9061 ctccacctcc gccagacatt cagcggtatc gcgggtcatg agatcatttt gcgattgtga
     9121 aacgttagag agacgcggag tttcgtgata cagcgccaga caaatggcgt ccagcagggt
     9181 agttttcccc gcaccggttg ggccggtaat ggcaaacagc ccgttgctgg cgaacggctc
     9241 gcgggtgaaa tcaatcttcc attcgccttt taatgagttc aggtttttca ggcgcaggct
     9301 gagaattttc atgcttcgga ttctccggcg agggtatgca acgtcgtggt gaaaagatgc
     9361 tgcagacgtt gctgctgcga ttcctccagt tcttccagtg ccagacggcg attgaacacc
     9421 tcttcgacgc tgagttcgct gagggtttca cgctgttggc tggctaacac acgctcgcgc
     9481 tgttcacgac tccggcgtac cagcaatact tcaacaggca atgattcggt taatgcctgg
     9541 attttgcgct gaatatcatg cagatactca tcagtagtga tttcgatatc cagccagaca
     9601 ggcggctcct gtgatacatc gcgccactgt tccagctgtg cggtaatcga cgccagatcg
     9661 cctttcagca ccgccatggg ttgcgttacc ggtacgttca ggttttccac gctctctaat
     9721 ttgccgtttg aaaatgtcac cagatggaca tatttactct ttccgcattc atcaaaactc
     9781 agtggaatgg gggagccgca atagcgaaca tgttccatgc cgccaataat ctgtgcgcgg
     9841 tgaatatgcc cgagcgcgat gtagtcggct ggtggaaagt tttgtgccgg aaacgcgtcc
     9901 agcgtgccaa tataaatgtc acgcacggcg tcacttttac tggcccccac ggtcgttaaa
     9961 tgtcccgtgg cgatgatggg cagaggctga tcgccgcgca gtttgcaggc atcggcatag
    10021 tgttgttggt aataatcggt aatcgctgcc agtaaatgct gctgtttttc aataccgtta
    10081 agccccgcct ggctggtaat aatgtcacgc ggacgtaaaa acggaatggg gcacagcacg
    10141 gcgcctggcg tcccgtcgcg acgaggcaag atttgcggcg catgtccggc gctggcgacc
    10201 acggtagtat tgaggaacgc catgatatcg cgcgattcat tcagcgtggc gaccgagtca
    10261 tggtttcctg ccagtaccac cagatgacag ccagtttgct gtaaattgac aacaaaacgg
    10321 ttgtataacg tgcgggcgta actgggcggc gagccggtat cgaaaacatc accagcaaca
    10381 ataatcgcat ccacctgatg ggtttgtgct gtttccagca gccagtcaag aaaagcctga
    10441 tgttcagctt cgcggctttt actgtagaag ttctggccga gatgccagtc tgaggtgtga
    10501 aggatgcgca taacggttcc ctggcgaaaa agcatgggcg cgattatacc caaacagatg
    10561 tgccatttgc ttttttctgc gccacggaaa tcaataacct gaagatatgt gcgacgagct
    10621 tttcataaat ctgtcataaa tctgacgcat aatgacgtcg cattaatgat cgcaacctat
    10681 ttattacaac agggcaaatc atggcgagac gtattctggt cgtagaagat gaagctccaa
    10741 ttcgcgaaat ggtctgcttc gtgctcgaac aaaatggctt tcagccggtc gaagcggaag
    10801 attatgacag tgctgtgaat caactgaatg aaccctggcc ggatttaatt ctccttgact
    10861 ggatgttacc tggcggctcc ggtatccagt tcatcaaaca cctcaagcgc gagtcgatga
    10921 cccgggatat tccagtggtg atgttgaccg ccagagggga agaagaagat cgcgtgcgcg
    10981 gccttgaaac cggcgcggat gactatatca ccaagccgtt ttcgccgaag gagctggtgg
    11041 cgcgaatcaa agcggtaatg cgccgtattt cgccaatggc ggtggaagag gtgattgaga
    11101 tgcagggatt aagtctcgac ccgacatctc accgggtgat ggcgggcgaa gagccgctgg
    11161 agatggggcc gacagaattt aaactgctgc acttttttat gacgcatcct gaacgcgtgt
    11221 acagccgcga gcagctgtta aaccacgtct ggggaactaa cgtgtatgtg gaagaccgca
    11281 cggtcgatgt ccacattcgt cgcctgcgta aagcactgga gcccggcggg catgaccgca
    11341 tggtgcagac cgtgcgcggt acaggatatc gtttttcaac ccgcttttaa cgccttgctc
    11401 atcggacgca gagcagggct tatgatttct taactggagt atcttacgtg ctggaacggc
    11461 tgtcgtggaa aaggctggtg ctggagctgc tactttgctg cctcccggct ttcatcctgg
    11521 gtgcattttt tggttacctg ccctggtttt tgctggcatc ggtaacggga ctgcttatct
    11581 ggcatttctg gaatttattg cgcctttcat ggtggctgtg ggtggatcgt agtatgaccc
    11641 cgccaccggg gcgtggtagc tgggaaccgc tactatacgg cttacaccag atgcagctgc
    11701 gaaataaaaa acgccgccgt gaactgggca atctgattaa acgctttcgt agcggcgcgg
    11761 agtcgctgcc cgacgcagtg gtgctgacca cggaagaggg cggtattttc tggtgtaacg
    11821 gtctggcgca acagattctt ggtttgcgct ggccggaaga taacgggcag aacatcctta
    11881 acctgctgcg ttacccggag tttacgcaat atctgaaaac gcgtgatttt tctcgcccgc
    11941 tcaatctggt gctcaacacc gggcggcatc tcgaaattcg cgtcatgcct tatacccaca
    12001 aacagttgct gatggtggcg cgtgatgtca cgcaaatgca tcaactggaa ggcgcgcggc
    12061 gcaacttttt tgccaacgtg agccatgagt tacgtacgcc attgaccgtg ttacagggtt
    12121 acctggagat gatggatgag cagccgctgg aaggcgcggt acgcgaaaaa gcgttgcaca
    12181 ccatgcgcga gcagacccag cggatggaag ggttggtgaa gcaattgctg acgctgtcga
    12241 aaatagaagc cgcaccgacg catttgctca atgaaaaggt tgatgtgccg atgatgctgc
    12301 gcgttgttga gcgtgaggct cagactctga gtcagaaaaa acagacattt acctttgaga
    12361 tagataacgg cctcaaggtg tctggcaacg aagatcagct acgcagtgcg atttcgaacc
    12421 tggtctataa cgccgtgaat catactccag aaggcacgca tatcaccgta cgctggcagc
    12481 gagtgccgca cggtgccgaa tttagcgttg aagataacgg accgggcatt gcaccggagc
    12541 atattccgcg cctgaccgag cgtttttatc gcgttgataa agcgcgttcc cggcaaaccg
    12601 gcggtagcgg attagggtta gcgatcgtga aacatgcggt gaatcatcac gaaagtcgcc
    12661 tgaatattga gagtacagta ggaaaaggaa cacgtttcag ttttgttatc ccggaacgtt
    12721 taattgccaa aaacagcgat taatccgcct ttgtcatctt ttattgccat aagccagtcg
    12781 atgctggctt attttctttg cagtcaaaat acgggcgtta gattttacaa cggttggtga
    12841 ttttttgttc gcatgattag ccatgtcttt ttcacggaaa tagtgtttta tactggttgg
    12901 tgatttctta tcgctatata cctctggttt ttagatccct ccttgcttta aaacgttata
    12961 agcgtttaaa ttgcgcttca ggtgctgtca tactgactgc attaacgcgg taaatcgaaa
    13021 aactattctt cgccgcgcct ggttgggagt atttcccgct aaaattgttt aaatataccg
    13081 ctgtatcatc cccagggatt ggcacaaaaa tttaacgtta caacaccaca tccacaggca
    13141 gtatgattta tgacccatca attaagatcg cgcgatatca tcgctctggg ctttatgaca
    13201 tttgcgttgt tcgtcggcgc aggtaacatt atttttcctc caatggtcgg cttacaggca
    13261 ggcgaacacg tctggactgc ggcattcggc ttcctcatta ctgccgttgg tctgccggta
    13321 ttaacggtag tggcgctggc aaaagttggc ggcggtgttg acagcctcag cacgccaatc
    13381 ggtaaagtcg ctggcgtact gctggcaacg gtttgttacc tggcggtggg gccgcttttc
    13441 gctacgccgc gtacagctac cgtttctttt gaagtgggga ttgcgccgct gacgggtgat
    13501 tccgcgctgc cgctgtttat ctacagcctg gtctatttcg ctatcgttat tctggtttcg
    13561 ctctatccgg gcaagctgct ggataccgtg ggcaacttcc ttgcgccgct gaaaattatc
    13621 gcgctggtca tcctgtctgt tgccgcgatt gtctggccgg cgggttctat cagcacggcg
    13681 actgaggctt atcaaaacgc tgcgttttct aacggcttcg ttaacggcta tctgaccatg
    13741 gatacgctgg gcgcaatggt gtttggtatc gttattgtta acgcggcgcg ttctcgtggc
    13801 gttaccgaag cgcgtctgct gacccgttat accgtctggg ctggcctgat ggcgggtgtt
    13861 ggtctgactc tgctgtacct ggcgctgttc cgtctgggtt cagacagcgc gtcgctggtc
    13921 gatcagtctg caaacggcgc tgctattctg catgcttacg ttcagcacac ctttggcggc
    13981 ggcggtagct tcctgctggc ggcgttaatc ttcatcgcct gcctggtaac ggcagttggc
    14041 ctgacctgtg cttgtgcaga attctttgcc cagtacgtac cgctctctta tcgtacgctg
    14101 gtgtttatcc tcggcggctt ctcgatggtg gtttctaacc tcggcttaag ccagctgatt
    14161 cagatctccg taccggtgct gaccgctatt tatccgccgt gtatcgcact ggttgtatta
    14221 agttttacac gctcatggtg gcataattcg tcccgcgtga ttgctccgcc gatgtttatc
    14281 agcctgcttt ttggtattct cgacgggatc aaagcatctg cattcagcga tatcttaccg
    14341 tcctgggcgc agcgtttacc gctggccgaa caaggtctgg cgtggttaat gccaacagtg
    14401 gtgatggtgg ttctggccat tatctgggat cgcgcggcag gtcgtcaggt gacctccagc
    14461 gctcactaaa tcactgaaca tttgttttaa ccacggggct gcgatgcccc gtggtttttt
    14521 attgtgttga tgggttagga attgatggaa agtaagaaca agctaaagcg tgggctaagt
    14581 acccgccaca tacgctttat ggcactgggt tcagcaattg gcaccgggct gttttacggt
    14641 tcggcagatg ccatcaaaat ggccggtccg agcgtgttgt tggcctatat tatcggtggt
    14701 atcgcggcgt atatcattat gcgtgcgctg ggggaaatgt cggtacataa cccggcagcc
    14761 agctctttct cgcgttatgc gcaggaaaac ctcggaccgc tggcaggtta cattaccggc
    14821 tggacctact gctttgaaat cctaattgtc gccatcgccg atgtgaccgc ttttggtatc
    14881 tatatgggtg tctggttccc gacggtgccg cactggattt gggtactgag cgtggtgctg
    14941 atcatttgcg ccgtaaacct gatgagcgtg aaggtcttcg gggagctgga attctggttc
    15001 tcgttcttta aagtcgccac catcatcatc atgattgtcg ctggtttcgg catcatcatc
    15061 tgggggattg gcaacggcgg gcaaccgacc ggtattcata acctgtggag caacggcggc
    15121 ttcttcagta acggctggct tggcatggta atgtcgttgc aaatggtgat gtttgcttac
    15181 ggtgggatcg aaattatcgg gattaccgcc ggtgaagcga aagatcctga gaaatcgata
    15241 ccgcgtgcga ttaactccgt gccgatgcgt attctggtgt tctacgtcgg tacgctgttc
    15301 gtcattatgt ctatctaccc gtggaatcag gttggcactg ccggtagccc gttcgtgctg
    15361 acgttccagc atatgggcat tacctttgcc gccagcattc ttaactttgt tgtgctgact
    15421 gcttcgctgt cggcaattaa cagtgacgta tttggcgtag gccgtatgct ccacggtatg
    15481 gcagagcagg gcagcgcgcc gaaaattttc agcaaaacgt cgcgtcgcgg tattccgtgg
    15541 gttacggtgc tggtgatgac taccgcgctg ctgtttgcgg tgtatctgaa ctacatcatg
    15601 ccggaaaacg tcttcctggt gatcgcttcg ctggcaacct tcgccacggt atgggtgtgg
    15661 attatgatcc tgctgtcgca aatcgccttc cgtcgccgtt tgccgccaga agaagttaag
    15721 gcgctgaaat ttaaagtgcc gggtggggta gcaacgacca tcggcgggct gattttcctg
    15781 ctctttatta tcgggttgat tggttatcac ccggatacgc gtatctcgct gtatgtcggt
    15841 ttcgcgtgga ttgttgtgct gttgattggc tggatgttta agcgtcgcca cgatcgtcag
    15901 ctggctgaaa accagtaatc cttgcgttct gtaagccggt tcaggaggaa atcctgatcc
    15961 ggctattccg aaagttatcc gcccccgtcc tcctccccca aatatccttc agatgatgag
    16021 tgatcctgca ttaggctatg gcaaggtgat cagattttca tcacagggga attatgatgt
    16081 taaatgcatg gcacctgccg gtgcccccat ttgttaaaca aagcaaagat caactgctca
    16141 ttacactgtg gctgacgggc gaagacccac cgcagcgcat tatgctgcgt acagaacacg
    16201 ataacgaaga aatgtcagta ccgatgcata agcagcgcag tcagccgcag cctggtgtca
    16261 ccgcatggcg ggcggcgatc gatctctcca gcggacaacc ccggcggcgt tatagtttca
    16321 aattgctttg gcacgatcgc cagcgttggt ttacaccgca aggcttcagc cgaatgccgc
    16381 cggcacgact ggagcagttt gccatcgatg taccggatat cggttcacaa tgggctgcgg
    16441 atcagatttt ttatcagatc ttccctgatc gtttcgcccg tagtcttcct cgtgaagctg
    16501 aacaggatca tgtctattac catcacgcag ccggacaaga gatcatattg cgtgactggg
    16561 atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat ctggacggga
    16621 taagcgaaaa actgccgtat ctgaaaaaac ttggcgtgac ggcgttgtat ctcaatccgg
    16681 tgtttaaagc tcccagcgta cataaatacg ataccgagga ttatcgccat gtcgatccgc
    16741 agtttggcgg tgatggggcg ttgctgcgtt tgcgacacaa tacgcagcag ctgggaatgc
    16801 ggctggtgct ggacggcgtg tttaaccaca gtggcgattc ccatgcctgg tttgacaggc
    16861 acaatcgtgg cacgggggga gcttgtcaca accccgaatc gccctggcgc gactggtact
    16921 cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg ccgaagctgg
    16981 attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt attgtccgcc
    17041 actggctgaa agcgccgtgg aatatggacg gctggcggct ggatgtggtg catatgctgg
    17101 gggaagcggg tggggcgcgc aataatttgc agcacgtagc cgggatcacc gaagcggcga
    17161 aagaaaccca gccggaagcg tatatagttg gagagcattt tggcgatgca cggcaatggt
    17221 tacaggccga tgtggaagat gccgccatga actatcgcgg cttcacattc ccgttgtggg
    17281 gatttcttgc caataccgac atctcttacg atccgcagca aattgacgcc caaacctgta
    17341 tggcctggat ggataattac cgcgcagggc tttctcatca acagcaactg cgtatgttta
    17401 atcagctcga cagccacgat actgcgcgat ttaaaacgct actcggtcgg gaggttgcgc
    17461 gcctgcccct ggcagtggtc tggctgttca cctggcctgg tgtaccgtgc atttattacg
    17521 gtgatgaagt gggactggat ggcaaaaatg atccgttttg ccgtaagccg ttcccctggc
    17581 aggtggaaaa acaggatatg gcgttattcg cgttgtacca gcgaatgatt gcgctgcgta
    17641 agaaaagtca ggcgctgcgt cgtggcggct gtcaggtgct gtatgcggaa gataacgtgg
    17701 tggtatttgt ccgcgtgctg aatcagcagc gtgtactggt ggcaattaac cgtggcgaag
    17761 cctgtgaagt ggtgctaccc gcgtcaccgt ttcttaatgt cgtgcaatgg caacgcaaag
    17821 aaggccatgg gcaactgaca gacgggattc tggctttgcc tgccatttcg gctacggtgt
    17881 ggatgaacta aagcatctgt tttatttgtt acgtcgttag tcaccagccc tgacactacg
    17941 cagcgcaaaa aagctgctca atgtcagggc tgaatttgtt aaatatttgt cccgatcgat
    18001 atgagcgatt tttggtattg tttgcccatg atgttatgta caacatgctc caggataacc
    18061 ctgtagtgga gcaaagatat gacaatttca aattcgaatt tattaaaacc gtttattact
    18121 ggttctaagt tgctaccttt taa
//
LOCUS       DAZUGK010000087        17688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZUGK010000087 DAZUGK010000000
VERSION     DAZUGK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.087"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..557
                     /locus_tag="ACHY5W_002567"
     CDS             54..557
                     /locus_tag="ACHY5W_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFS4447448.1"
                     /translation="MGMIGYFAEIDSEKINQLLESAEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(624..2081)
                     /gene="nuoN"
                     /locus_tag="ACHY5W_002568"
     CDS             complement(624..2081)
                     /gene="nuoN"
                     /locus_tag="ACHY5W_002568"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFS4447449.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2088..3617)
                     /gene="nuoM"
                     /locus_tag="ACHY5W_002569"
     CDS             complement(2088..3617)
                     /gene="nuoM"
                     /locus_tag="ACHY5W_002569"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFS4447450.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3781..5622)
                     /gene="nuoL"
                     /locus_tag="ACHY5W_002570"
     CDS             complement(3781..5622)
                     /gene="nuoL"
                     /locus_tag="ACHY5W_002570"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFS4447451.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5619..5921)
                     /gene="nuoK"
                     /locus_tag="ACHY5W_002571"
     CDS             complement(5619..5921)
                     /gene="nuoK"
                     /locus_tag="ACHY5W_002571"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFS4447452.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5918..6472)
                     /gene="nuoJ"
                     /locus_tag="ACHY5W_002572"
     CDS             complement(5918..6472)
                     /gene="nuoJ"
                     /locus_tag="ACHY5W_002572"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFS4447453.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6484..7026)
                     /gene="nuoI"
                     /locus_tag="ACHY5W_002573"
     CDS             complement(6484..7026)
                     /gene="nuoI"
                     /locus_tag="ACHY5W_002573"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFS4447454.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7041..8018)
                     /gene="nuoH"
                     /locus_tag="ACHY5W_002574"
     CDS             complement(7041..8018)
                     /gene="nuoH"
                     /locus_tag="ACHY5W_002574"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFS4447455.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8015..10741)
                     /gene="nuoG"
                     /locus_tag="ACHY5W_002575"
     CDS             complement(8015..10741)
                     /gene="nuoG"
                     /locus_tag="ACHY5W_002575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFS4447456.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10794..12131)
                     /gene="nuoF"
                     /locus_tag="ACHY5W_002576"
     CDS             complement(10794..12131)
                     /gene="nuoF"
                     /locus_tag="ACHY5W_002576"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFS4447457.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTYLINGIQPNLLKERW"
     gene            complement(12128..12628)
                     /gene="nuoE"
                     /locus_tag="ACHY5W_002577"
     CDS             complement(12128..12628)
                     /gene="nuoE"
                     /locus_tag="ACHY5W_002577"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFS4447458.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12631..14433)
                     /gene="nuoC"
                     /locus_tag="ACHY5W_002578"
     CDS             complement(12631..14433)
                     /gene="nuoC"
                     /locus_tag="ACHY5W_002578"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFS4447459.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14527..15189)
                     /gene="nuoB"
                     /locus_tag="ACHY5W_002579"
     CDS             complement(14527..15189)
                     /gene="nuoB"
                     /locus_tag="ACHY5W_002579"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFS4447460.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15205..15648)
                     /gene="nuoA"
                     /locus_tag="ACHY5W_002580"
     CDS             complement(15205..15648)
                     /gene="nuoA"
                     /locus_tag="ACHY5W_002580"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFS4447461.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16278..17216)
                     /gene="lrhA"
                     /locus_tag="ACHY5W_002581"
     CDS             complement(16278..17216)
                     /gene="lrhA"
                     /locus_tag="ACHY5W_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFS4447462.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4402 a         4943 c         4591 g         3752 t
ORIGIN      
        1 ttccctcatc tgttttaaga tgcgaaacat gaaattcaac ggaggaaaaa taaatgggaa
       61 tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt gagtctgctg
      121 aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga ctggatattg
      181 ataaacgctg ggatttttta cactttgggc tgacaggcac ctctgctttt gatcccgcga
      241 agaatgatcc gctgagtcgt gccgtgcttg gtgaacacag cctcgaagat ggcatagatg
      301 gattccttgg attgacctgg aatcaggagt tagccgcgac aatcgaccga ctggaatcac
      361 tggatcgaaa tgaactgcgt aaacagtttt ccattaagcg actcaatgaa atggagattt
      421 atccgggagt gaccttttca gaagagttag aggggcaact ctttgcctcg atcatgcttg
      481 atatggaaaa gctgattagc gcgtatcgac gtatgctgcg gcaagggaat catgcgctga
      541 cggttattgt tgggtgatgg tgtcagggca ccaaaaaaaa ccgccgaaca tgtcggcggc
      601 tttctgactt acaaactaac agattacatc agtggcattg ctaaccgcac aatgctaatc
      661 agcggttgtg gccatacacc cagcaccagt accaacagtg cagagatcag caccacgata
      721 ccgcccgcgc tgtactgcca gtttgacggc gcatcgcgac ccggttgttc cggggcgtga
      781 agatacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga accgacaacc
      841 acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac gtagaactta
      901 ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac cgtcatcact
      961 gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga atcagcatcc
     1021 gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag gctgctgaac
     1081 agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccagt ttgcagcgca
     1141 atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag cagacgtttg
     1201 atattggtct ggctcagcgc catcaggtta ccgaagatga tggaggcaaa ggcgataatc
     1261 gccagcacca cacgaatcgc ttcgctgtca cccaccggtg cgtagaggaa cagacgcatc
     1321 actacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc
     1381 gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag tttgaagccg
     1441 aggccaacaa tcatcagacc gaaacctgcc agcaacagcg gctcgttgag cataccgtcg
     1501 ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata caccagcgcc
     1561 ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt gatactggct
     1621 tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc
     1681 agttcaatac cgaggaacag agacgccaga tggttggcat tcgccagcag gatcccgccc
     1741 agggcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata gccttcaagc
     1801 cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc
     1861 atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc ctggccaaca
     1921 aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa
     1981 tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc gacgatcagc
     2041 aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta cggccttgta
     2101 gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg cgaggtatcc
     2161 agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa caggatcata
     2221 aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct tttcgcttta
     2281 ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac cagcccaaag
     2341 gtagaaataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat cataaattcg
     2401 ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa cagcgacagt
     2461 gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg
     2521 atacgttcat aaagctgacc gcagagaata aacagacccg ccgccgacaa gccgtgcgca
     2581 atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat agcaatcagc
     2641 acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc ggtctgggcg
     2701 aaggccatcc acgcaccgta gaagatgccg ataacaccca gccacatagc gattggcgcg
     2761 aactctgccg acgcatttgg gaacagcggc agggagaaac gcagcaaacc gtaagcggca
     2821 gttttcagca agatccccgc gaggtcaacg gaaccggcgg tcggagcctg ggagtgcgca
     2881 tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc gatgaagaag
     2941 cccagcatca acagatattc cacaccactg gacattggcg tattcaacag ctcttcatag
     3001 ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc
     3061 aacatcacca gaccactcgc ctgtgtgtaa atgaagaact tggttgccgc cgtgatacgc
     3121 gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat cggcaccagc
     3181 atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac gccgataacg
     3241 ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc gatctctttc
     3301 cacgaacaga gtaccgccag cacaccgagc agaccggtca gcacgaccat tagcagcgac
     3361 agcccgtcaa tggcgagatg gatagagata ccaaaacgcg ggatccacgg catgtcgaat
     3421 tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac
     3481 agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca gcgcggcacc
     3541 ttgacgccaa agcgttcggt ctgccagcac aggaagccgc cgataaaggg aattaatatt
     3601 agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag gaccggattt
     3661 tcaacgaatt ctcaagacag aaaattctct tcggactggg ggtagtgtcg gatgcgccgc
     3721 gaaggcggct tatccgacct acgttcttat atgcccgggg gcaatcccac aatccttaac
     3781 tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca tggatgccac
     3841 ataccagcgc agataaccgt tctcgcttaa cagcaggcct ttacctgcaa agcgggaaag
     3901 aaccgccgga atgttcatca ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc
     3961 caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccagg cgttgtacca
     4021 ccaggtactt agcaggcggc ccggcgcact gttggcgatg gaggtcacca gagtacgttt
     4081 acccagccac agccaggcag ccagcagaat gccgacaatc gcgaccacgc cagaggtaat
     4141 ttccagggtc aacatgctgc cgtgcgccag ctccgtagtt tgcggaagca cgccctgcag
     4201 cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc aacagcacaa tcagcggcag
     4261 gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat
     4321 gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac ctgccaccat
     4381 cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc
     4441 cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc agagataaac
     4501 cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt gatggcaggc
     4561 cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt gggtcatcaa
     4621 gtgaaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt agccaatctg
     4681 gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagtg cggcaaaacc
     4741 ggccagcagc agcgtaaccg ccccaacaat gcccaccaga tgcagaactt ccggcgtcat
     4801 caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca tcgttgcggc
     4861 gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcggcaagcc atgtctgcaa
     4921 cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg cccacatcag
     4981 catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc ggaagttcag
     5041 ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg
     5101 ggtcacgacg aacgctttca ttgccgctgc gccattcttc ggatcggtgt aatagaaacc
     5161 gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca gcagcaggtt
     5221 gtcggcaagc accagaacta ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg
     5281 agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtggatca ggaaacccac
     5341 accagtgacc accgagagca tggtcagcga caggccgtcc aacaccaggt taaaaccgat
     5401 gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt
     5461 agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca cagagccaac
     5521 gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca gcaggacgaa
     5581 gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat ctcacttact
     5641 gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc aaggccgata
     5701 ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc gtcggtctga
     5761 ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat gatttccaga
     5821 ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc aagaacgaat
     5881 aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc tccgtttttc
     5941 ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct tcacgaccga
     6001 cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc accgccagta
     6061 cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta ccgtcgatac
     6121 cttgatcgtt aacaccgaga atggcgtaaa caatcaccac caacatgatg gctgacaaaa
     6181 ttgccggacc aatccacacc tgcggtttca gccactggcg ttcttgttcg atttctgaac
     6241 cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca cccgcgtaga
     6301 cgataatttc cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg
     6361 ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg gtgatcactc
     6421 gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc attgcccctc
     6481 tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg cttcgccctt
     6541 atctttgccg tcgattgcca tacctgccat ccggtagaag ttatactccg ggtatttgcc
     6601 cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct tgtattcccc
     6661 catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt cgcacaaacc
     6721 acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc ggataccagc gaccgtcttt
     6781 ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcgc agaggttaca
     6841 ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac cacgataacg
     6901 gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg
     6961 caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca attcttttaa
     7021 ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc cgttaccagc
     7081 aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac ctggtcataa
     7141 cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa cgcggttttc
     7201 agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa gaacagcgtc
     7261 accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa cagaccgaac
     7321 ttcatgccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc ttccggctga
     7381 tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat aaaaccaaag
     7441 aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt catgttgaat
     7501 gaaccggctt gcgccaccac gcccatcagg gaaagcccga ggaacacttc gtagctcagg
     7561 gtctgcgcag aagcacgcat cgcacccagc agcgagtatt tgttgttact tgaccagccc
     7621 gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa aatcccgatg
     7681 ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc cagcagcagc
     7741 gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa tttcgggatc
     7801 cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga accaccccag
     7861 ccaacacggt taggtccgta acggttctgg aacagaccca gcaggcgacg ttcgccaaag
     7921 ctcatgaatg ccccgcaggt gacaaccacc agcaggatca ccaccgcttt gaggatggtc
     7981 agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc ttgagatcct
     8041 caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc aagcccacct
     8101 gccctgccgt cagcccttca gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac
     8161 taaaggagac gcgtgtacct gcgtttacac ccaacttcgc ggcatccgct gggttgagtt
     8221 tgatgtacgg ctgcggcata cgactctgga agaccggagc acgctgtgac aattcatcac
     8281 tgccaaacag gtggtagtac ggcgcaatac gccatttccc gtcctgcggc tggaagcgtg
     8341 ccggtacgct ggtgaagtaa tccagaccat tttcgctggt ttcaaacaga cgcacgcccg
     8401 gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac gcctgcgggg
     8461 agttccagcc cggagcccag gcaaacggca cttgcgaacg atgcgcggtc ggctggttgt
     8521 taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc ggctcatgaa
     8581 cgctgatatt ggcgcgcatg gcagtacgac cgctgtaacg gtgcggttca cgggccagtt
     8641 tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgatacct gccagttccg
     8701 ggattttcgc cacaacagcg tcaatcacat ggtcgagctg cgtccagtcc acttcacggc
     8761 tcagcagagt gctgtgcagc gagtgtaacc agcgccagct ttccagcatg acagttttgc
     8821 tgtcgtaata agccggatcg taaacctgga agaaacgttg ggcgcggcct tcgttgttga
     8881 tcaccgtacc gtcgctttca gcaaagctgg cggcggaaag caccagatgg gcgttttcca
     8941 tgatcgctgt gcgttgatgg tcaaccacca tcaccagcgg cgctttagcc agcgcagcat
     9001 tcacgcgggt agcggatgcg tgacgatgca gatcgttctc cagcaccacc accgcgtcgg
     9061 cgcgtccggt ttccagttcg gttaacgctt cttcaagcga accgccaccc ataatgccca
     9121 gccccatgct gttgacggaa cgggcaatca tggtgatacc gacgtcagcg ccgcgacctt
     9181 tcagggcttt tgcgacgtta gccgccgcct gaatcacctc taagctaccg gcgttcgtcc
     9241 cggagataat caacggtttc ttcgcacccg ccagtgcctg cacgatgacg tcgattttgc
     9301 tttgcagttc aggttcaata ccgtcaaccg ctggcgcgga gttgtccagc gcatgggcga
     9361 tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat
     9421 catccagacg ggtgtcatca acgttggtaa caaacagcgg atgcttagca cgttgaccga
     9481 tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgccgcc atttcgcgcg
     9541 ctttaccttt caccgcctga cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt
     9601 cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc ggagtataaa
     9661 tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga
     9721 taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg ctttccacgc
     9781 tggcgcgcgg agaaccaatg ccgatcactt tcttcgactg acgcagaata tctgctgcgc
     9841 cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc tgtactggct
     9901 gacgcggacg atctttcagg ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga
     9961 agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttca ccataacgct
    10021 cgccggggct gatgttacag ccgatggaac attgctggca gatgctcggc gcaaactgca
    10081 tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc
    10141 aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga
    10201 agtagacgtt gtcgtgtgcg ccataaacgc ccagatctgt accgtcagcg taatctttgt
    10261 agtaacgcac gcagcggtag caggcgatgc agcggttcat ttcgtgagag atgaacggcc
    10321 ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg
    10381 tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc ggacagtcgt
    10441 gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc gcttcttcgt
    10501 cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc accaggcgac
    10561 cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc
    10621 ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac agacaagctt
    10681 ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga attgtagcca
    10741 ttagcatgct tccagttttc tcagtcagag actgagcgtt aatcgaaatt cggttaccag
    10801 cgctctttca gcaggttcgg ctgaatccca ttaatcaaat aggtattgct gaacggctgt
    10861 ttgatccccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa cggctccact
    10921 gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca cagttgctca
    10981 agtgtttcga tatcgcccgg ctgaccttcg ccacgctcca gcgcacgcag aattttcacg
    11041 ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg ggcgaaaaac
    11101 tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc catcgccagc
    11161 gccgtaccca gacggcttcc cgctttaccg atactttcga attccatcgg cagatcaagg
    11221 tgcgcttcgg tcaggaagtc ggtgcccgcg ccgcctggct gccaggcttt gaatttcaga
    11281 ccgtcacgca taccaccggc gtaatcttcg aggatctcgc gcgcggtagt accaaacggc
    11341 agttcccaca ggcccggatt tttcacccga ccggagaagc ccatcagctt ggtgccagca
    11401 tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat cgccggaacg
    11461 ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc ggaagttgcc
    11521 gggaatggcg gtttcgaacg cgggttagcg cgacgccctt ccagggagtt gattaacgct
    11581 gtttcttcac cgcagatgta gcgccctgcc ccggtatgga cgaacagttc gaaatcgaaa
    11641 cctgttccca taatgttttt gccgagcagc cccgcttcag tcgcttcggc aatggcgcgg
    11701 cgcagattca ctgccgcttc aatatattcg ccacgcagga agatgtagcc acggtaagct
    11761 ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag ttgctccatc
    11821 aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag caggtaacgg
    11881 atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga aaagcccgcg
    11941 ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc cggagacagc
    12001 ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc gtccagccac
    12061 actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg agtacggata
    12121 atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc agatgcgcgt
    12181 gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg cagcaagttg
    12241 gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc agctttttct
    12301 caagcgccgc ctgaataccc tgataaccgt tgatatgaca gaccacgctg tcgcaataac
    12361 ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt gccacacctt
    12421 cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca ccatccggca
    12481 cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca cgcgggtctt
    12541 cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct
    12601 cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca taacaaaatc
    12661 gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg ccgccggaat
    12721 ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca tggtgctacc
    12781 gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct ggaaagattc
    12841 attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca gggtttcgat
    12901 atgttgcagc gtgcgctctt tcggcggtgg cgtggtcagc gggtgatccg ctttgaacgg
    12961 gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc gcagctcttc
    13021 cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa
    13081 gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt cgatcccggt
    13141 agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc cataggcggc
    13201 aacgccctgg gaacgacctt tcagaatggt attttgcagc gccgctttct cgtaagacgc
    13261 cagacgtttc ggcatccagt ctaggaactc acgcagcaga cgatcccagc cgcgcggcag
    13321 gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac ccgtgatcgc
    13381 ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc
    13441 gccgacgtcc tgaataaagg tcgagatata cagcaggtgg ctgttgatgc ggaacagttc
    13501 ggagagcatt acgcgaatga cgttaacgcg atccggcacg gtgatcccgg ccagtttctc
    13561 taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcga tacggtcggt
    13621 atacggaatg tagctgtgcc aggactggcg ttcgcccatt ttctccgcac cacggtggtg
    13681 gtagccgatg tctggtacgc agtcgacaat ctcttcgcca tcgagctgca aaacgatacg
    13741 gaaagcaccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga agtcctcgtt
    13801 ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt ccatctccag
    13861 atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc
    13921 tttacgcagc gggtgacctt tccaggtttg cggcatcatg attcggcgca ggttcgggtg
    13981 accgtcgaat gtaatgccaa acagatccca ggtttcacgc tcataccagt tagcgttcgg
    14041 gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca ccttcagcat
    14101 gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat ccgcggcagg
    14161 taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa acagcatgac
    14221 gtaaggtttc ggcagtttct ttaagaaatc gcccacttcc agtaattgtt cacgcttgat
    14281 ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg
    14341 gttgcgcagt tcgccaatca ccggatcatc aagatgatcg cgggtctgcc aggcgggttc
    14401 ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt gactgtcggc
    14461 gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt tgccgacagg
    14521 cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt
    14581 gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa ccacccagga
    14641 gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct gcatgtacgc
    14701 ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt tatcgacgcc
    14761 ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggcac ccattgagat
    14821 aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg ccattttggt
    14881 aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc
    14941 tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca
    15001 gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt taaccatgtc
    15061 attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt cggttacgat
    15121 ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt
    15181 ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt tcgtttccgg
    15241 gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag
    15301 ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa agcctaccca
    15361 gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat
    15421 aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg cggagccgac
    15481 cgagtcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa
    15541 aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa gaaagatagc
    15601 gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg cttactcatc
    15661 aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg attcatgggg
    15721 aggaataaaa aaaaccttac aatcactgta gaaattcttt tatacagcta attgatgtgg
    15781 tcttttactc ctttctataa ccttttgtca actttaacaa aagtttcttc acattagttt
    15841 acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt gacgactcca
    15901 ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta
    15961 ttttgacagg aatttgtgtc gattcctccc cctaaaagag agtattttct tgatctgtga
    16021 cacgcttttg tcattccata acaaaaacgc agcaacaaat ttacgtattt tttaacatca
    16081 ttgtagcagg tgattttttt caggcgatta tttgtgcgta cggggcgtga atctctggtg
    16141 attgaaaaat gaacagtttt gtacgttctg cacgatgtga agaaggttct ttactaaaaa
    16201 aaagccgctg gggtctaaaa cacccccagc ggctcgtttt tacactattg ttccaggaat
    16261 tatctatggt ccgtcgatta ctcgatatcc ctttcaatta acaatgagtc atctccttcc
    16321 ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc
    16381 acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt
    16441 agcggcggta agccatctac tccactcagc acacgcaagt ccggactcat catctcgacc
    16501 ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag
    16561 gcgacataag ccagtcgcca gggaatatct gctttattaa gcgtcgccag caccatatcg
    16621 cgaaacggac ttggatcatc cagcaataca agagggatcg gctcaccttt ttgcaatacg
    16681 tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct
    16741 gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat ttctgccatg
    16801 taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga
    16861 tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg
    16921 ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg caggattttc
    16981 ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga
    17041 gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac
    17101 tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca
    17161 aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat
    17221 tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat
    17281 tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc
    17341 atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca
    17401 cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac
    17461 atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct
    17521 ggtcatacgc gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt
    17581 catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc
    17641 tgatgcaaag ttatgttatg ttaggcaaag taatcttcta ttattaat
//
LOCUS       DAZUGK010000088        17545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZUGK010000088 DAZUGK010000000
VERSION     DAZUGK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.088"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(135..1163)
                     /gene="epmB"
                     /locus_tag="ACHY5W_002582"
     CDS             complement(135..1163)
                     /gene="epmB"
                     /locus_tag="ACHY5W_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS4447463.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1205..1771
                     /gene="efp"
                     /locus_tag="ACHY5W_002583"
     CDS             1205..1771
                     /gene="efp"
                     /locus_tag="ACHY5W_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4447464.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1823..1948
                     /gene="ecnA"
                     /locus_tag="ACHY5W_002584"
     CDS             1823..1948
                     /gene="ecnA"
                     /locus_tag="ACHY5W_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS4447465.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2059..2205
                     /gene="ecnB"
                     /locus_tag="ACHY5W_002585"
     CDS             2059..2205
                     /gene="ecnB"
                     /locus_tag="ACHY5W_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS4447466.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2386..2703
                     /gene="sugE"
                     /locus_tag="ACHY5W_002586"
     CDS             2386..2703
                     /gene="sugE"
                     /locus_tag="ACHY5W_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS4447467.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKALPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2700..3233)
                     /gene="blc"
                     /locus_tag="ACHY5W_002587"
     CDS             complement(2700..3233)
                     /gene="blc"
                     /locus_tag="ACHY5W_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS4447468.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYHHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3322..4455)
                     /locus_tag="ACHY5W_002588"
                     /pseudo
     CDS             complement(3322..4455)
                     /locus_tag="ACHY5W_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            complement(4518..4877)
                     /gene="frdD"
                     /locus_tag="ACHY5W_002589"
     CDS             complement(4518..4877)
                     /gene="frdD"
                     /locus_tag="ACHY5W_002589"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS4447469.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4888..5283)
                     /gene="frdC"
                     /locus_tag="ACHY5W_002590"
     CDS             complement(4888..5283)
                     /gene="frdC"
                     /locus_tag="ACHY5W_002590"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS4447470.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5294..6028)
                     /gene="frdB"
                     /locus_tag="ACHY5W_002591"
     CDS             complement(5294..6028)
                     /gene="frdB"
                     /locus_tag="ACHY5W_002591"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS4447471.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6021..7829)
                     /gene="frdA"
                     /locus_tag="ACHY5W_002592"
     CDS             complement(6021..7829)
                     /gene="frdA"
                     /locus_tag="ACHY5W_002592"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS4447472.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8154..9131
                     /gene="epmA"
                     /locus_tag="ACHY5W_002593"
     CDS             8154..9131
                     /gene="epmA"
                     /locus_tag="ACHY5W_002593"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS4447473.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9350..10852
                     /gene="yjeM"
                     /locus_tag="ACHY5W_002594"
     CDS             9350..10852
                     /gene="yjeM"
                     /locus_tag="ACHY5W_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFS4447474.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11003..14326)
                     /gene="mscM"
                     /locus_tag="ACHY5W_002595"
     CDS             complement(11003..14326)
                     /gene="mscM"
                     /locus_tag="ACHY5W_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFS4447475.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14348..15316)
                     /gene="asd"
                     /locus_tag="ACHY5W_002596"
     CDS             complement(14348..15316)
                     /gene="asd"
                     /locus_tag="ACHY5W_002596"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFS4447476.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15413..16465)
                     /gene="rsgA"
                     /locus_tag="ACHY5W_002597"
     CDS             complement(15413..16465)
                     /gene="rsgA"
                     /locus_tag="ACHY5W_002597"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFS4447477.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKLAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16560..17105
                     /gene="orn"
                     /locus_tag="ACHY5W_002598"
     CDS             16560..17105
                     /gene="orn"
                     /locus_tag="ACHY5W_002598"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFS4447478.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPAILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17316..17391
                     /locus_tag="ACHY5W_002599"
     tRNA            17316..17391
                     /locus_tag="ACHY5W_002599"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17349..17351,aa:Gly,seq:gcc)
BASE COUNT         4062 a         4525 c         4615 g         4343 t
ORIGIN      
        1 ccgtactcac tctatataaa cagaaaagaa aggttaatta ttggtgttgg ttatataaag
       61 agtaacaaca gcaatgtata tgaataatta aaggttatgc aacgggcaaa gattaaactt
      121 ccctgtttaa tcagttactg ctggcgtagc tggagatcca gcggcgtttt gctgggttcg
      181 ccgccaattt ctcgcgccag tttcggcacc agatatcccg acaccagtgt cagcaactca
      241 cgcataatct gccgtgcttc gtcatcactc accataaaat gcgccgcgcc ctgtacttta
      301 tcgagcacat gcaggtaata cggcatcacg cctgcatcga acaacgcatt actcaggttt
      361 gccagcgttt gtgcgttatc gttcacacca cgtaacagaa cgctctggtt cagcaaagta
      421 acgcccgccc ggcgcaactt agccatcgcc tgacggaatg tttcatctat ctcattggca
      481 tggttgatgt gattcaccag caagatttgc agcgtagaat gtgaaaagcg ttcaaccagc
      541 gcatctgtga tgcgtgccgg gatcacaatc ggcagacggc tgtgaatccg cagacgtttg
      601 atatgcggga tggcttccag ttgtgtgagc aaccagtcca gctcgtgatc tttcgccatc
      661 agcggatcgc cgccggagaa aatcatctcg tccagttccg gatgcgcagc aacatactca
      721 agtgcagttt gccagttacg cttgttgccc tgattttcgg cataggggaa gtggcgacgg
      781 aagcaatagc ggcaatttac cgcgcagccg cctttgacca gcaaaagcgc ccggttgtgg
      841 tatttatgca acaaaccagg cactacgctg tgctgttctt ccagcgggtc ggtggagaat
      901 ccgggcgcga tggcaaactc atcttgcgag gtaagtacct gacgcaaaag cggatcgtcc
      961 ggattgcctt tctccatgcg atcgataaat gagcggggca cacgcagggc aaacagcttt
     1021 ttggcgctgc gtccggccaa cagtttttcg tccgcgtcta tattcaaaag acgcagaagt
     1081 tcatcaggat cggtcacaac atcggcaagt tgcgttaacc aatcttctct ggatggggta
     1141 tttagggtta caatatgcgc cattttgtgg cttagctacc aattaacaaa tttcagaggg
     1201 ccttatggca acgtactata gcaacgattt tcgtgctggt cttaaaatca tgttagacgg
     1261 cgaaccttac gcggttgaag cgagtgaatt cgtaaaaccg ggtaaaggcc aggcatttgc
     1321 tcgcgttaaa ctgcgtcgtc tgctgaccgg tactcgcgta gaaaaaacct tcaaatctac
     1381 tgattccgct gaaggcgctg atgttgtcga tatgaacctg acttacctgt acaacgacgg
     1441 tgagttctgg cacttcatga acaacgaaac tttcgagcag ctgtctgctg atgcaaaagc
     1501 aattggcgac aacgcgaaat ggctgctgga tcaggcagag tgtatcgtaa ctctgtggaa
     1561 tggtcagccg atctccgtta ctccgccgaa cttcgttgaa ctggaaatcg ttgataccga
     1621 tccgggcctg aaaggtgata ccgcaggtac tggcggcaaa ccggctaccc tgtctactgg
     1681 cgctgtggtt aaagttccgc tgttcgtaca aatcggcgaa gtcatcaaag tggatacccg
     1741 ctctggtgaa tacgtctctc gcgtgaagta atgcggttgt ggtgcggcct gcaggctgca
     1801 ccatcactta ttcaggtcag agatgatgaa acgccttatc gttcttgttt tgcttgccag
     1861 cacgctgctc acgggctgta acaccgctcg cggtttcggc gaagacatca aacatctcgg
     1921 caactccatc tcccgcgctg ccagctaatt tttcttctct tccgaaaaat catcagattt
     1981 ccgtcatttt tggcgatgtt gtctattatt aatttgctat aggcaaacat aaataacatt
     2041 acctaaaagg aagacgttat ggtgaagaag acaattgcag cgatcttttc tgttctggtg
     2101 ctttcaacag tattaactgc ctgcaacacc acgcgtggcg ttggtgaaga catttctgat
     2161 ggcggtaacg cgatttctgg cgcagcaacg aaagcgcagc aatagcaata agcaataacg
     2221 gtacgacagc tgtgtcgtgc cgtttgtttt tttgcgatag tcacaaaggt aatagttgaa
     2281 attcctctgc cacctggcaa aatatccgtt caaccatcag ctttgcagga cgacctgcaa
     2341 acgcctcttt tcaccgggga cggccccaat tctccggagc ctgatatgtc ctggattatc
     2401 ttagttattg ctggtctgct ggaagtggta tgggccgttg gcctgaaata tacccacggc
     2461 tttagtcgtt tgacgccgag tgttattacc gtgacggcga tgattgtcag tatggcgcta
     2521 cttgcctggg cgatgaaagc gttaccagta gggacggctt atgccgtgtg gacgggtatt
     2581 ggcgcagtcg gcgcggccat caccggcatt gtgctgctcg gtgagtccgc taacccgatg
     2641 cgtctggcga gtctggcgtt aatcgtattg gggattattg gtctgaaact cagcactcac
     2701 taactaccgg gctgctgtac ccaaataaac ttactgacat caaacccttc ccgggtcgcg
     2761 actgccagca tctcctgttt cacttcgtca gaaatggttg gcgtgcggga gagtatccac
     2821 aggtagtcgc ggtccgggcc gcaaaccagc gcatggtggt attcccgatc gagtgcaata
     2881 acgttataac cgccatagaa aggaccaaag aatgacactt tcagcgcagc gcgagttggt
     2941 gcgccggtaa agtacgcttt cccttcactc tgctgccaca ttcctctgtc agggttatag
     3001 cctttattaa tgacattcag gccgccatca tccctcaggc tgtatgttgc ggtgactttt
     3061 tccagtccac gttcaaagcg gtgatcaaaa cgggcaatct cataccaggt accgagatag
     3121 cgtttagcgt cgaaattatt cactacggtc acgccacgcg gcggcgtagg agaactgcag
     3181 gcaacgacca gaaatgcagc tgtcgctgcg gcaacgagag ggagtaagcg cataaatgtt
     3241 tccttactgg tttttttcta agtgtagatg gcagcaagga aaagcggaga aaaggtccga
     3301 aaattcggac ccgatgaaat tttactgcag agcgttgaga atctgccagg cggcggcgac
     3361 tctcgctgga ttgggatagt ttttgttagc cagcatcacg atacccagct ctttttctgg
     3421 aataaacgcg acatagctac caaatccgcc ggtcgccccc gttttatgca cccatgatgc
     3481 gtgtactgca ggagttggag gcgtaatcgc ttttacgggg cgtgctgcca gtgcaatttt
     3541 attgtcactg ccgttaacga tgatgtcagg atttaccggc cagtccagca tttcccagcc
     3601 cagaccctga tacatatcgc cggtttgcca gtagcgagat tgtgccagtt gtatcccttg
     3661 gtgaagtgtt ttgtcgttga tatcacgggg attcatattg cttcgtaccc agcaggccat
     3721 atcttcaatg gtcgacttca caccataagt ttcagcatct aacgcccctg gcgaaacatg
     3781 cactgcctta ccttcgcgat atccccaggt gtaattcttt tcttctgcgg gcggtacatt
     3841 aatccacgta tggttgagtt tgagtggctg gaagacacga gtttgcatcg cctgctcaaa
     3901 actcaaacca gacggcttca cagccagtgc gccgaacaaa ccgatactgg agttggcata
     3961 cagacgttat gttcctggag cccatgcagg ctgccagttt tgatagaagc gcagcaagtc
     4021 gcttgaggat ttcacctcat ccggcacctg caatggcagg ccgccagcgg tgtaggttgc
     4081 gagatgtaat agtgtgatcc cgttccactg tttagcggta agttcaggcc agtattttgt
     4141 tgcgggatcg cttaacttga tttcccctcg agcaatagcg tcgccaccaa gcacgcccgt
     4201 aaatgttttg ctgaccgaac ctaactcaaa caacgtttgc tgtgtgacgg gctgcttttt
     4261 ggcgatgtcc gcatagcccc agataaagta ataaggttta ccctgataaa ttaccgccac
     4321 cgccataccc gggatctttt gttgctctat aagcggggta attgtgcgat gcacaatatc
     4381 gttgattttt tggggggcag caaatgtgga gcaagaggcg gtaattaata aggcgcagag
     4441 cgtcgttttg aacatagggt ctggtttcca tacaaaacgg cccgccatag gcgggccgga
     4501 tttacattgg cgatgcgtta gattgtaacg acaccaatca gcgtgacaac tgtcaggata
     4561 gcagccagac cgtagaaaac ccatttgccc gcaggtacgt ggattttcag atcgtgcatc
     4621 gcgtggtgca tacggtgtaa accacaccac agcggcagaa cgatcatcag gaacaggaat
     4681 acgcgaccaa tgaagctctg cgcgaacgcc agaacgcgct cgtagctcag tgcatcaccc
     4741 ggaaacagcc ccagtggcag cagaataccc accagcagga tcatcaccgg cgcaatgatg
     4801 gcgctccaca taccaccggc cccgaagagg ccccagaata ccggttcgtc agaacgcttt
     4861 ggatttggat taatcatctc aggctcctta ccagtacagg gcaacaaaca ggattacgat
     4921 ggtggcaact acagttaccg cccagagact tttgataatt ggctctggtc ccattttttc
     4981 gtcttttaca atgatattgg ccgctttcgg tgccagttca aaccaggttt tggtgtgcag
     5041 cagagctgcc gccagagtga tcaagttaat gatcacaata accgggtttt gtaaaaagtc
     5101 gacgaatccc gcccaggctt ccgggccatt tttcagggca aacagcccga aaatcagttc
     5161 aatgctgaac cacacagccg gaaccgccgt gccttcgcgc agcatgtaaa agcgataaaa
     5221 cggcaatttt ttccaccagg tggacgtcat tggccgtaca tacggtttac gtttagtcgt
     5281 catgttgcac tccttagcgt ggtttcaggg tcgcgataag aaagtctttc gaactttcta
     5341 ctttgccctg ctgaatggcc gcagccggat cgacgtgttt cgggcagact tcggagcagt
     5401 agcccacgaa agtacagctc catacgccgt tctggctgtt caactgcgcc atacgctcct
     5461 tcttaccgtg gtcgcggcta tcttcgttat aacgatgcgc cagcgtaatg gcagccggac
     5521 cgatgaactc tgggttcagg ccaaactgcg ggcacgcggc gtagcacaga ccacagttga
     5581 tgcaaccgga gaactggtga tacttcgcca tctgcgccgg ggtctggatg ttagtaccct
     5641 gatccgcggt gcgggagttg ccgatgatgt acggtttgat cgcttccaga ctttcgatga
     5701 agtgggtcat atcgaccacc agatcgcgtt caatcgggaa gttagctaac gcttcaacct
     5761 tcataccgtc ggtgtaatca cgcaggaagg ttttacatgc cagttttggc acgttgttaa
     5821 ccatcatgcc gcaggaacca caaatcgcca tacggcagga ccagcggtag ctcaggtccg
     5881 gtgccaggtt gtctttgatg tagcccagcg catccagtaa tgaggtagtt gcgtcataag
     5941 gcacttcata gaatgcgcta tgcggtgcgg tatcgacttc cgggttatag cgcaccacct
     6001 caattttcag gtttttcatc tcagccattc gccttctcct tcttattggc tgcttccgcc
     6061 ttatcggctg catccgcttc gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc
     6121 ttcacgtcgc tgtactccag gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt
     6181 ttgaggaagt tgacgtcgtc acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg
     6241 cgggactctt tacgtgccat tgcggagtgc gccatacatt cagcaacgtt cagaccatgg
     6301 cccagttcaa tggtgtagag caggtcggtg ttgaacacgc tggaagtgtc ggtgatgcgc
     6361 acgcgcttga agcgttcctg aagctctgcc agcttgtcga tggttttctg catcagttcc
     6421 ggcgtacggt aaataccgca gccttcttcc atagccaggc ccatttcgtc gcggatcttc
     6481 gcccagtttt cgccgccatc ctggttaacc agatctttca gacgttgttc aacgccagct
     6541 gcctgcgctt caattgccgc ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct
     6601 tgttcaccgg ccagacgacc gaagaccacc agttccgcca gggagttgga gcccagacgg
     6661 tttgcaccgt gcagaccaac agaggaacat tcacccacgg cgaacagacc tttaatgcgg
     6721 gtttcacagt tctgatcggt ttcgataccg cccatggtgt agtgtgcggt cggacgtacc
     6781 ggaatcggtt ctttaaccgg atcgacgcca acgtacgctt tcgccagttc gcagatgaac
     6841 ggcagacgtt catgcagttt tttctcgccg aggtgacgca ggtcgagata aaccacatca
     6901 ccacgtggcg tggagatggt gttgccttta cgccattcgt gccagaaggc ctgagaaact
     6961 ttgtcgcgtg gacccagttc catatatttg tttttcggct cgcccagcgg agtttccggg
     7021 cccatgccgt aatcttgcag ataacggtag ccatttttgt tgaccagaat accgccttca
     7081 ccgcggcagc cttcggtcat caggataccg gaacctggca gaccggttgg gtgatactga
     7141 acgaattcca tgtcacgcag cggaacgccg tggcttagcg ccatacccat accgtcaccg
     7201 gtaacgatgc cgccgttggt gttgtaacga taaacgcgac ccgcaccgcc agtagccata
     7261 acgaccgcgt tagcacggat ctgcaccagc gtgccttcca tcatgttcat tgctaccagg
     7321 ccgcgaacat gaccatcatc aaccagaata tccagtacga aatgttcgtc aaaacgctgg
     7381 atctgcggga attgcagaga ggtctggaac agcgtgtgca gcatatggaa gccggtctta
     7441 tcggcggcga accaggtacg ctcgattttc atgccgccga agcgacgtac gttgacgcta
     7501 ccatccgggc gacggctcca tgggcatccc cacagttcca gttgggtcat ttcggttggg
     7561 cagtggtgga cgaaataatc cacgacatcc tgctcacaca accagtcgcc acccgctact
     7621 gtatcgtgaa agtgatattc gaagctgtca tgatcctgcg cgacagcggc ggagccccct
     7681 tctgcagcaa cggtatggct acgcatcggg tatacttttg agattagtgc gatttttgca
     7741 ttcggatttg cctgcgcggc agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca
     7801 atggcaagat cggcttgaaa ggtttgcacg acattcctcc agattgtttt tatcccacag
     7861 ccccgtactt cagggtaagt gcctgaaagt tacggtctgc gaacgctatt ccactgctcc
     7921 tttataggta caacagtata gtctgatgga taagtctgaa atttgacgag atcgattttt
     7981 ttagtgcgca agagggctaa attatcactg aagatgatta atttaattac taaaccatca
     8041 gatcgtgctt tttttagtca ctgcccaccg ctgtttgatt cctgcgtaaa atttgtctcg
     8101 ccgtcgcgtt gcgagtagac ttcgtgccct tgtcaaaaac tggagattta actatgagcg
     8161 aaacggcatc ctggcagccg agcgcatcca ttcctaactt attaaaacgc gcggcgatta
     8221 tggcggagat ccgtcgtttc tttgccgatc gtggagtgct ggaggtggag acgccttgta
     8281 tgagccaggc gacggtaacc gatattcatt tggtcccgtt tgagacacgt ttcgttggcc
     8341 ccgggcattc gcaggggatg aatctctggt taatgaccag cccggaatac catatgaaac
     8401 gcctgctggt tgctggttgt gggccggtat tccagctgtg ccgcagcttc cgtaatgaag
     8461 agatggggcg ttatcacaac cctgagttca ctatgcttga gtggtatcga ccgcactatg
     8521 atatgtaccg gttgatgaac gaggtggacg atctcttaca acaggtgctg gactgcccgg
     8581 cagcagaaag cctttcttat caacaagctt tcttgcgtta tctggaaatt gacccactct
     8641 ctgccgacaa aacgcaactg cgggaagtgg cagcgaaact ggatttgagc aatgttgctg
     8701 ataccgaaga agaccgcgac acgttgctac aattgctgtt tacctttggc gtagagccaa
     8761 atattggcaa agaaaaaccg acctttgtgt accactttcc agccagccag gcatcactgg
     8821 cgcaaatcag taccgaagat catcgggtcg ctgaacgctt tgaggtttat tataaaggta
     8881 ttgagctggc gaatggtttc catgaattga cggatgcccg tgagcagcaa caacgctttg
     8941 aacaagataa ccgtaagcgc gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc
     9001 tgattgaagc cttgaaagtc ggtatgcctg actgttccgg cgtggcatta ggcgttgatc
     9061 gtctggtgat gttggcgctg ggcgcggaga cactggctga agtcatcgcc tttagcgttg
     9121 accgggcata attctgaaaa attactgaat taacagcgaa gaatggcgtg ataagcgcca
     9181 ttctgtagca tatgttttca tttttccgtt aaaggccaca atttaagact ggatatttga
     9241 tatcatccag gtatcaattc tgtattggtt tttacgctgc ctgctcaatg ttgcgcagtg
     9301 cagttcagtg ggcacgtgtt atacacgcgc tgaaatgaag gatggtttca tgcctcacac
     9361 gataaaaaag atgagtctga taggactcat attgatgatc tttacttccg tatttggatt
     9421 tgccaatagc ccatcggctt attacttaat gggttatagt gcgattccct tttatatatt
     9481 ttctgcattg ttattcttta ttccattcgc cttaatgatg gctgaaatgg gagctgctta
     9541 tcgcaaagaa gaaggtggta tctattcctg gatgaataat agtgtcggac cacgttttgc
     9601 cttcattggt acgtttatgt ggttttcctc ttatatcatc tggatggtga gtacctccgc
     9661 gaaagtttgg gtaccgttct caacattcct ctatggtagc gacatgaccc agcactggcg
     9721 tattgccgga ctggagccta cgcaggtggt tggtctgctg gcagtggcat ggatgattct
     9781 ggtcaccgtc gttgcttcta aggggattaa taaaattgcc cgcattactg cggtgggcgg
     9841 tattgcagta atgtgtctga atttagtatt gctgttagta agcattacta ttttgttatt
     9901 aaatggtggg catttcgcgc aggatattaa tttccttgca tcaccgaacc cgggttatca
     9961 gtccggtctg gcaatgctat cgtttgtggt atttgctatt tttgcctatg gcggaattga
    10021 agcggttggt ggtctggtcg ataaaacgga aaatccagaa aagaactttg ccaaaggtat
    10081 tgtttttgcc gctattgtta tttcaatcgg ttattcgctg gcaatatttt tatggggcgt
    10141 cagcacaaac tggcagcagg tattaagtaa tggttccgtt aaccttggca atattaccta
    10201 tgtgctgatg aagagcctcg gggtgacgct gggtaacgca ctgcatttgt cacctgaagc
    10261 gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta
    10321 taccggtgcg ttctttacgc tttgctattc accgctgaaa gccatcatcc aggggacgcc
    10381 gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc cgtctatcgc
    10441 catgtggatg cagtgcgggt tggttactgt cttcatcctg ctggtttcgt ttggtggcgg
    10501 taccgcatcg gctttcttta acaagctgac gctgatggcg aacgtgtcta tgacgcttcc
    10561 ttacctgttc ctcgcgctgg ctttcccatt ctttaaagca cgtcaggatc tcgacagacc
    10621 atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acagtggttg tcgtactggt
    10681 ggtgacattt gcgaacgtct tcaccatcat tcaacctgtg gttgaagccg gagactggga
    10741 cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga tggcgattta
    10801 ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact gatcctatga
    10861 aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc gtatggatga
    10921 tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga gccacgttga
    10981 catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg cccgcagacg
    11041 tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg aagggcggga
    11101 atggcatatc gataccgtgg gcatggaagc cagccagaat cagctggtgg atctcatggc
    11161 gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc
    11221 cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc agcgagcagc
    11281 gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg gcaggggccg
    11341 gaatcgtcaa caccacgcgc gtgaccgagt cagagagcga ccagttgata aactgctcgg
    11401 taataaacgc cttgttcggc acgataattt ctttgcggtc ccagtcgctg attgtagtgg
    11461 cgcgggtatt aattttcgtc acgctaccag taagatcgcg gatcgttacc gtatcgccaa
    11521 tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg aaaatttcct
    11581 gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat ttcgaccact
    11641 caataccaat catcgagaag ccgaccagcc cgccaatcag catcaacagg tatttggtga
    11701 tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa atcgccagtt
    11761 ccagcagcgc gggcaggttg cgcaccagtt gcgtggtgat gataaacacc agaatggcaa
    11821 tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc gtggaggtga
    11881 catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct gaccacagca
    11941 caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc aaggactgcg
    12001 cactgatggc atcgagatcg acttcgcttt catcgacttc aattgcccct tccgggctac
    12061 tgtgatgatg tgcttcctct tcgccacgcg cacgttgcgc taacatctct gcccggcgat
    12121 gcttcgcccg gtcaaacgcc agcctgcgtc gttggatcag catccagcgg cggataacgt
    12181 gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc gctaacagcg
    12241 cctgtgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt ggcgcgccaa
    12301 tcatcatgtt ccacaacata tggttggtaa tattgtcgcc gctgccttct ttgttgagat
    12361 acagcgggat cccggccttt ttcaggctga gggtgaccac cgccagcgca ccgcaaatga
    12421 ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgaga ttatcgaaca
    12481 tcatcagcgc cataatcagt ggcacaataa gcccgatgct catcaggtag tagcgcatcc
    12541 cacgagaaac acgttcgcgc ggccagccaa aatgagcgat aaacaagccg ttcgggcggg
    12601 caaaggtagc gcaaatcatc actacccaca gcagcggcac ggtggctgtt acgccatcgc
    12661 caatcgcgac cgccagcgga taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc
    12721 acagcaccgg cagcggtgac gcgacgagaa tcgaccagaa aagagtgcgc aacgtcagcc
    12781 agaagtgatc ctgagtcact ttgccgactt tcgccgccga acgttcaaga aaacgggtga
    12841 aatagcggcg cgagtaaata ctgcaaccga ccagaatcaa cgcgccaaac agcggcaaaa
    12901 tcgtctcttt gctggtcagc atcatcacac tggctttgcc caactgactg aaggtgtcca
    12961 gcgagataag gcggcgcaga tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc
    13021 gcacgtcaga ggtccagaac agatagcggt gcgtcgcttc gttcacctct ttcagcgcat
    13081 cctccagttg cccgttagag actttcagct tggtcagttc cagcagtagt gtgtcgccac
    13141 cctgcaacaa tgagttcagc aactcacgct gagtgcgtag ctgtgcttcg agaatacggt
    13201 tttgctcggc agtcagcggc tgaccgtcgg cctgatgaat ttgccgtagc agcggctgtt
    13261 tattaagcag atcctcataa cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa
    13321 gctgttgtgg tttcggcatt tccggcagcc gtgccacctg cgcccgcaac gcttcaccga
    13381 gcagattgga cgatcccagc cattgcgact gttcacgcag cgtattcaac gcctgccgga
    13441 cctgtaacgt ctggctggca gcctgacgct gttgcgaggc aacgagatcc atccgctgcg
    13501 cctgttgatt cagcgccgcc gatagttcgc ggttaatttt gaattgcgcg acgatatctt
    13561 tcggcaaatc ggcactgttt tctgctagta attcggtact ttccagcgcc cgctctgcct
    13621 caagctggcg ttggctgttc aattgattac gcaaggcctg caaatacgca tccagttgct
    13681 ggctctcttt ttccgccagc tctgagcgca agcgcgctaa ttcctggcgg ttattggcag
    13741 acagctgcgc cagttccagt tcatcaacga gcgctttcag gcgtgcggag tcagactgca
    13801 acgcgaaatt ttgtgcctga ttgagcggag tattgccggt aagcgttccc aggcggcgct
    13861 cgatctcgtt taactgacgg cgggcgtcgg tttgctgttg cggcagttga ttcagcgaat
    13921 cggcaatctc gcgggcgcgc tcctgctctt gctgggcctg acggctttta tccagcaact
    13981 gactgctgac ctggagaatt tcctgattca gcgcgtcggt agacatcccc ggcgacacgc
    14041 tgcgcggctc gtcacgcatg ttgtttaatt gtgcgcgcag agtagcggag agtttcggat
    14101 aattatcaat gacttcttga tattgcttga tgcgctcaag ggaacctttt cgttcctcaa
    14161 gcgcatttaa ggcagactgg agcgcctcta cgacttccgg ctgtgcgggt ttcgccgctt
    14221 ttgcctgctc cagttcctgg gtgatttgtt tgctatcggg ggccgtcgcg gcgtacgccc
    14281 cccaactgag gcaccaggcc atcagaaaag tgataatcag gcgcacgtca gcgtttcctt
    14341 tgatggatta gacctggtct tttttatcgt caaccaatgg gctggcgtcg tgttctgctt
    14401 cgatctcttc agcaggaagc ggggcgggtt cagcgtctgg cgtaacaaag gtttcggtag
    14461 atactgccag cggctggcca attttcgtga cagacaggct ttccagttgc tcaaccagat
    14521 tcactttacc tggtgcaaac aggttgataa cggtggaacc gagtttaaag cgacccattt
    14581 cctggccttt cagcagtgcc acagaaccgt cgttttcccc ggcaggccag gtccagcgct
    14641 tgatgatgcc ttcgcgcggc ggcgtaatgg tgcccgccca gacagtctca atgctgccta
    14701 caatcgtcgc cccgaccaga atctgcgcca ttgggccaaa ttcggtatcg aacaggcaga
    14761 tcacgcgttc gttacgggca aacagattcg gcacgttctg agcagtgaga tggttaacgg
    14821 agaagagatc gcccggcacg tagatcattt cacgcagaat accgttgcac ggcatgtgta
    14881 cgcggtggta gtcacgcggg gagaggtaag tggtcacaaa cgtaccgttg cggaacaggt
    14941 cagccatcag atagttgcct gccagtaggg cttcaaggct gtagttgtgg cctttggctt
    15001 gcaggatttt atcttcttcg attttaccca actggctgat aacgccatcg gcaggcataa
    15061 ccaggacatt cggatcggta tcgattgggc gtacttcgtc acgcagcgga cggacaaaga
    15121 attcgttaaa ggtgcggtag ctggcggtgt ccggcttttg cgcctctttc atgtcgacct
    15181 tgtagtattt aacgaacaga tcgataacca gttttgtcag ccatcctgcc cgcttgcttg
    15241 cgccccaacc cgccaggcga gtaagccata gtttcggcag aatgtactgt agcgaaagtt
    15301 taaatgaatt taacaaggta gcctccaggc cattgttttg tcgttcctga tccggcctac
    15361 atgccggatc ctgaaaaaaa ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat
    15421 ccgtatcaga aaagttttta cgcgttttta cctgcgccat gctttccaga atacggtgat
    15481 agttttcgaa acgggtttcc gcgattttcc cttcctcaac cgcttcccgg atagcgcagc
    15541 ccggatcggt atcgtgtttg caatcgcgat atttacacag acctaaatag tcatggaatt
    15601 cgacaaagcc ctgagtgatt tgttccggct ccaggtgcca gaggccgaac tcacgcacac
    15661 caggggaatc aatcacatca ccgccgtgcg ggaagtgata caggcgtgcg gcggtcgtgg
    15721 tgtgttgacc gagacccgag ttgtcagaga catcgttggt caggatctct ttttgcagcc
    15781 ccagcaacgc gttcagcagg ctggatttac caacgccaga ctgcccggca aaaatgctga
    15841 tgcgcccggt caacgcctct tccagcggtt ttagcccatc ctgagtatgg ctggaaacca
    15901 tcaatacgcg ataaccgata ttgcggtaga tatccatctg ctcgttgacg aacgccatgc
    15961 cttcgtcgtc cagcaggtct atcttattga gcacaataat cggctcaatc tgcaaggttt
    16021 cgcaggccac caggtaacgg tcgataatat tgagcgacag ctccggcaaa atggcggaga
    16081 caatgacaat ctggtcgatg ttggcggcaa taggtttcac gccgtcgtag aaatccggac
    16141 gcgtcaacac cgaggtgcgc tcatgcaccg cttccacgat ccctttgaca tttacgcctt
    16201 ccgccgccag tttacccgga cgccagacta cgcggtcgcc ggtcaccagc gaacggatgg
    16261 tacggcgaat attgcagcgg tgaacgtcgc catcggcgga ttccacatca gcgtgcatac
    16321 caaagcggct gatgacgata ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg
    16381 gcttctcctt agacgtttta agacgacgct ggtgattggc gttcacgcgg cgctgctggc
    16441 ctttggagag tttattttta ctcaatcgta ctgactcctg gtcgcccctg atgggcaaaa
    16501 catctatgat acacgcaatt gtggatcaat atagtcactg tgaatgggtg gaaaatagca
    16561 tgagtgccaa tgaaaacaac ctgatttgga tcgatcttga gatgaccggt ctggatcccg
    16621 agcgtgatcg cattattgag attgccacgc tggtgaccga tgccaacctg aatattctgg
    16681 cagaagggcc gaccattgca gtacaccagt ccgatgaaca gctggcgctg atggatgact
    16741 ggaacgtgcg cacccatacc gccagcgggc tggtagagcg cgtgaaagcg agcacgatgg
    16801 gcgatcgtga agctgaactg gcaacgctcg aatttttaaa acagtgggtg cctgcgggta
    16861 aatcgccgat ttgcggtaac agcatcggcc aggatcgtcg cttcctgttt aaatacatgc
    16921 cggagctgga agcctacttc cactaccgtt atctcgatgt cagcaccctg aaagagctgg
    16981 cgcgccgctg gaagccggca attctcgacg gctttaccaa gcaggggacg catcaggcga
    17041 tggatgatat ccgtgaatcg gtggcggagc tggcttacta ccgcgagcat tttatcaagc
    17101 tgtaaaattt ttgctggtca cgtaagcgcc ggaatccact tgcggcggcg tgattttgac
    17161 gctaaattga acgtcttgct gataatttca gcacttgaga taaaaactca aaaaaaattt
    17221 tttagggggg ttgcagaggg aaagatttct cgtataatac gcctcccgta acgacgcaga
    17281 aatgcgaaaa ttacgaaagc aaaattaagt agtacgcggg aatagctcag ttggtagagc
    17341 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttgaa
    17401 aagtgctgca aagcacagac cacccaatcg cagtatttcg aattgcgaca aggcggcaac
    17461 tgaacgaatc cttgggaact tacaaaagta agtgactaag gtgagtgaag gcagccaagg
    17521 cagtagcggt tcgaaagacg aagat
//
LOCUS       DAZUGK010000089        17539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZUGK010000089 DAZUGK010000000
VERSION     DAZUGK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.089"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..529)
                     /locus_tag="ACHY5W_002600"
     CDS             complement(<1..529)
                     /locus_tag="ACHY5W_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFS4447479.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDS"
     gene            complement(625..852)
                     /locus_tag="ACHY5W_002601"
     CDS             complement(625..852)
                     /locus_tag="ACHY5W_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4447480.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            1582..3195
                     /locus_tag="ACHY5W_002602"
     CDS             1582..3195
                     /locus_tag="ACHY5W_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447481.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDSIEFSGIRNNIINAVKRLESFEKWYETNKVLLVSNKTIRTEKIDVVVRLAGLKAYD
                     LQEGVPDGLKWKVKDGINERIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLM
                     EQKKKNKRFPYSKDRHAIQPLWSKCVIEQ"
     gene            complement(3288..3884)
                     /locus_tag="ACHY5W_002603"
     CDS             complement(3288..3884)
                     /locus_tag="ACHY5W_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447482.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(4014..5330)
                     /locus_tag="ACHY5W_002604"
     CDS             complement(4014..5330)
                     /locus_tag="ACHY5W_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4447483.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRFCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELACVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(5627..6391)
                     /gene="nfeF"
                     /locus_tag="ACHY5W_002605"
                     /gene_synonym="yqjH"
     CDS             complement(5627..6391)
                     /gene="nfeF"
                     /locus_tag="ACHY5W_002605"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFS4447484.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            6679..7302
                     /gene="nfeR"
                     /locus_tag="ACHY5W_002606"
                     /gene_synonym="yqjI"
     CDS             6679..7302
                     /gene="nfeR"
                     /locus_tag="ACHY5W_002606"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFS4447485.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(7409..8929)
                     /gene="aer"
                     /locus_tag="ACHY5W_002607"
                     /pseudo
     CDS             complement(7409..8929)
                     /gene="aer"
                     /locus_tag="ACHY5W_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            9347..10726
                     /gene="ygjG"
                     /locus_tag="ACHY5W_002608"
     CDS             9347..10726
                     /gene="ygjG"
                     /locus_tag="ACHY5W_002608"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFS4447486.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(10768..11100)
                     /locus_tag="ACHY5W_002609"
     CDS             complement(10768..11100)
                     /locus_tag="ACHY5W_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFS4447487.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            11319..12302
                     /gene="ebgR"
                     /locus_tag="ACHY5W_002610"
     CDS             11319..12302
                     /gene="ebgR"
                     /locus_tag="ACHY5W_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFS4447488.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPSSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            12486..15578
                     /gene="ebgA"
                     /locus_tag="ACHY5W_002611"
     CDS             12486..15578
                     /gene="ebgA"
                     /locus_tag="ACHY5W_002611"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFS4447489.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSSEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSNVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            15575..16024
                     /locus_tag="ACHY5W_002612"
     CDS             15575..16024
                     /locus_tag="ACHY5W_002612"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFS4447490.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            16087..17520
                     /locus_tag="ACHY5W_002613"
     CDS             16087..17520
                     /locus_tag="ACHY5W_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4447491.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVKLNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         4592 a         4422 c         4129 g         4396 t
ORIGIN      
        1 tgctatcgga gtaatatttt aacattccct ctatcgttgt atcattaaaa attgttcgac
       61 gtatctgcgg cggaacagga gagttattca gatgcgtttc aagctctgat tctgttgcct
      121 tgtaatcttt accggcacga atctctttat gaaattttga ttccagcgct ttttgttttt
      181 gctcccatat ttccttttct gtactgtaat tctcaaccag tttcgcgtat tcttccgcca
      241 gggcttcatc cctgagataa aatgctttca taaacacttt atccacggtc gttttccttt
      301 caccggaatc agccagaatc agagagtaaa tattaacggg cccacgtaaa tttccaggtc
      361 tgcacacgtc aatctgattc tgacaggcaa ttgacatcgc tgttaatgcg gatgtcgcca
      421 ccatagccaa aggagcctgt gtattttgtt gagtttcaat tattgcattt ctcaccagcg
      481 gtggtagtgc atatatcgga taaggatttt ctggtgctag taagcacata aagcctccat
      541 tttcattaat gggttagtaa agttgcagca atttccgcta caatagcgaa aatagctgct
      601 attcagactg aaaatacaaa cagttcatct ggtctgaatc caccagagaa ggtcacttag
      661 taaccagcca caagatttct ttccgatagt cttacgcggt gggtagtttc cctctcgttc
      721 aagtttccag gcttgtgaac gggaaattga ggtgatctct tttcgttcct tttcacgtac
      781 cagtctgtca ccctcagtcc cataggcttt caaaattact gaacgttgct catctgttaa
      841 tggaacccac atgacggccc tcctgatata tcaatgattc ttatctcagt tgtgagcatg
      901 aaatgactga atttcctgga caatccatag tggagattcc gttgtcatat tgctcacaga
      961 acacccatgg acagcaaggt acatacgaaa acaaatcaag tcaagttaag gcaagtaaaa
     1021 caatacttga ctcacaagtc cactcatagg tcaatatgtg ttgattagca acaaaacaac
     1081 caataatgca cattacaaac aatgatttta agccaagaag tggtttccac tgttgacaat
     1141 gacacaaaat gaatctagta tcgacttcat gttatgctca gataaacttc tgacagtgat
     1201 agtaaagaag agaaggaggg ggttaaattt gtgacaaaga tcacaaaaat aaattagttt
     1261 ttactattca caataggagg gaaagagagc caggcatttt acctgtcatt gaaatgacaa
     1321 caacagcttc aacagatcgg aatattcagg aggaggaaga agaaggagat ctgacgggtg
     1381 ttataattgt tacaacacgc tggaggaaaa ttatcgtaat aatagatgca ttctcaggta
     1441 taccctgtat caccccgaaa acacggcagg catccgtttt attagtagat gcagactcag
     1501 tatcaacgta tcctttcagg agataacatc gattttaact gccagtatcc ggtatgcggc
     1561 aaaaggaaac cttcttacat aatggtaaat cagactatgc aacaaaatca gtactatcgt
     1621 attttatccg gagaatcccg cccattccct ggcatatatg aggctgtttc cgaacaactc
     1681 cttttgctca tttttaaggc acgtcagcta tatcaggatc cagaatatcg cccctggttt
     1741 gaagaacagc acaaagttct cttggcattg cgtgataata cagagctcca gaaaattttt
     1801 aacaaacaaa gaaaagaaaa ttttgctctt ggcgcatcta catcgtctca acccactgct
     1861 cttactaatg cgcttaatgt tatgtgccat ttatacaatg gctttgatga aggaaatatc
     1921 agcctgaaaa gctggggagc gatgcaagag gtacagacaa caccagactt tttcagcctg
     1981 ggaccaagaa cctggaaaac acctgaactg gcaaagtttg taggagatga tttatttaat
     2041 attctgactg aatgtaaagc ctggagttca ccaattccag cagaagcccg gcaggggtat
     2101 tgccatcagg atctgtctgt tgcgcactcc tgcacctcag agttgctggc acacctgaag
     2161 cagtatcatg aaatagatga atcaacaata cttgccataa ccattggtct ggcggtcaac
     2221 gaattctacc agtataagca aaatattcca gaatggcttt cctgttttga gaataagaac
     2281 aaatggccct gggactgcag gaagtcagat actgaagatg tgcacagact gagagaacga
     2341 ctatcgataa tcggatgtac agaattcgta aaaaatatgt tctcagtcgc cagtcagcat
     2401 gaaataaaga caatgtacaa tatttatgtc catatttatg cagctgaaat ctgcggttgg
     2461 gtaaaaacaa gcatggcata ttttaaacca acagatttca tccagtacac atggaaattc
     2521 gcaacagtta ctccggataa agatgaaaaa accgagaata cgaccgcatg ggaaagggat
     2581 tattgtcaga taaccataca acgagatgaa ctccttagat tgattcgcac taatactctg
     2641 caggatgcag caattcatct ttcaggctgc ctgcagtatg catatctgga gtctgtaaag
     2701 aatgatattg actatcctgg aatagcccct aatgctgaag aaaagaaaat gaaatacaca
     2761 gattctattg aattttctgg aattaggaat aacatcataa atgcagtaaa acggctggaa
     2821 tcatttgaaa aatggtatga aactaataaa gttttgttgg taagtaataa aacaatacga
     2881 acagagaaga ttgacgttgt tgtacgactt gcgggactga aagcctatga tttgcaggaa
     2941 ggggttcctg atggtctgaa atggaaagtt aaagatggca taaatgaacg catcaaaacc
     3001 gataccagtt tatgcctgcc tcaggatatc agtgatacat cgctaaaccg ttatcgtcac
     3061 gctgtgacgc gcattgtcaa cgacgaaatc gacacattac tgatggaaca gaagaaaaaa
     3121 aataaaagat tcccctacag caaagatcga catgcgatcc aacctttatg gagcaaatgc
     3181 gtgatcgagc agtagagttc tgaggggata accgagctgt tatcccccgt ttattctgcc
     3241 aactctcagg tctgtcagag ttccaagaat acgatgcggc tggcaaatta tgcggctttt
     3301 ccattaacag attcggccac aagaacaaca ccgtgtttat ttcgatcagg atgttcactc
     3361 cagatttctg attcaggaat actttccccg tcatagcaga tccgatcgcc atcaaacgga
     3421 taataccgcg cggaatcagt atctaaaata aacactttca gattcgttga acgacgcgct
     3481 atatgctgcc agagggcttt acctccttca tattgctcgt tatcacttaa tagtataacg
     3541 cccgcttttg tggctaccgt ttcatataca aacgtcgcaa ggccaacatc acgtacagcc
     3601 ccatcaacaa caatcccttc aatctgagcg acgtcctgct cttcccagaa tttccggaca
     3661 aacacccctt caagctgtga gcgacgctga agctccagag tggcaacaac tttatggcga
     3721 atatcaccat caataacaga aggtgcagct tcgattaccg cagcgaacgg agtctttgga
     3781 tcccagatga tctggtaaag cacgccctga atagttactc catccagtat cgaaaaacga
     3841 cgagctacag tagcagggga gtaagacatt ggaattacag ccatttttga atcccctctg
     3901 agtaatacag gcacaaaaat ttacctcaat aattatagta aacaatacaa gaactaggtg
     3961 catcaacaat acccatcaat tgtacgcaat tcgtacagta accaggaact ccctcacctt
     4021 ctgccaaaca gattgacaac attattgcct gtttgctgtt gatagttatt aacccgatcc
     4081 tcccagatct caagtgctct gcgtttttct ggcaaataat cgtatcgatc ataatgtttt
     4141 gaactgacat cgttcaacgc atgattttga atacgatcgc ggatttcctt actgattcca
     4201 gcttctccca ttaacgtttt acaggtccga cgtaagtcac gtgctgtaaa aattttaaag
     4261 tcaggattac aagcccggaa atacatgata gcgcgagcca gactgtccgt acgaaccgga
     4321 cgcttaccat tagtcgaaag cgggaaaatg taagggctcg atttttcttt tgttaactcc
     4381 tgcacacagg ctaattcctg taatgctgat tcagtcaacg gtatcaaatg ctctcgttta
     4441 tttttagaga catccgcagt caccagcagc gttttttgct gaaaatcaac atcacaccac
     4501 tgactggcga tcatttcaaa gggacgttgc cctccggtat agatacagaa tcgaatcaaa
     4561 tgttgcatca ataatccaac gttggttgct tcagcaaaat gttccattag aaaacgcaac
     4621 tcttcgagtg tcagccaggt gtcaccgact ttttctgcag atgactgttt cggtatcact
     4681 gatacaggat tggcctccag cccaaaacta atccctacac ttgtgttcat cggatcgtta
     4741 tcagctttca gaccatagtt aaatgcagcc atcaggtaag aacggatccg gttagagtga
     4801 accacagcac cgcgctgaat gatcccggcc aagatatgtt taatctgtcc ggaagtcaca
     4861 tctttagctt tagtctcacg ggggatcact gtatagcatt cattttcaag gcgtttcaga
     4921 acatcctgcc atgttctctt attatcaatt ttcattttgt taacataacc atgaatcaat
     4981 gcttcaaatg aaccctgtga ttgatggagt tgtctccgct gctgttctgc aagctgttgt
     5041 cgctccagtt ctgtctgggg atcctttcct tcaatgagcc aggcaccata cttttttgcc
     5101 agttcgttcg ctgtggccag tttcatctca ggccatatac ccaattgaat gaatctttct
     5161 tttgtccctt tttccacgta atagcggaaa tagaaaattt tgctgccaga agactgaact
     5221 ttgacgccaa gtcgtccagt gccacgttga gagctattgc tccagacata gaacgatgtc
     5281 ttttttgctt ttaatgctcg aatagctgtt tcagttagat tgacggccat agaacctctt
     5341 ttctaaatca gtaacaatca aaataactcg taaatttggg tcaggtaatg ggtcaggtaa
     5401 cgatcgtaca atggagaaaa aacggaacca agcggaaaca gaagaaatta cataattagt
     5461 tgattataaa caattaataa aacaattacg aacaaaccaa aacaatacag aatggacttg
     5521 cctcatgact cataatcgct tggtcgctgg ttcaagtcca gcaggggcca ccagatatag
     5581 caaaggctga cgagaaatcg tcagcctttt tctttttata tatcagttac tttgcgtgcc
     5641 agtaagccgc tgcacgtacc cgctgtgggt catactgttc cgcttcaaag cggcggctta
     5701 aattcttaac gactttacct tcgccggtta tccagatgaa gtaatcatcg gcagggattt
     5761 gcatctgcgc cagacgcgca tctaccgcct gctcatcatg tgccagccat tcgatattaa
     5821 aaccatcaag gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta accagcgcac
     5881 taacttgcgg tttaacggca agtttgctca acgtttccag gcggcggcgc aatgcgggca
     5941 ttccggattc atcgcagaca tacagctgat acgcgtaatc ttccggcacc accagcgaac
     6001 cgcgcggacc tgccaccgtg agtttatcgc ccggttgggc ctgcattgcc cagctactgg
     6061 cgacgccgtc ttcgtgaata aagaaatcaa tcgccagttc atggcgtagt tcgtcataca
     6121 acggcgtata gtcacgcgac ggtgggcgtg gtccttccgg ccagacgatg ccctcttccg
     6181 taaccgttgg cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt
     6241 caaagccgcg cgatgtaaaa ccgtccagcg cctcgccgcc aaggacaatg cgctgaaaac
     6301 cggcgctgat acgctcaacg cgtagcacag tcagttcacg gaagcgcaga tcattgcgaa
     6361 cgcgctgcgg gtagcggggg gtgttattca ttgttatcgc cttcgtgatg gtaatcagat
     6421 atatctaaat aaaattcgca aatgataatg attgttaatc atgataaatg caagcgattt
     6481 gtagaactga tatgtctata gtctgataag acgaaccgcc tcttctcagg catcattact
     6541 caacgccgga tgcggcgtga acgctttatc cggcctacgt gtgagatgag tccacgttta
     6601 aaaatcattt ttacacttgc aagagcgctc atatcagata taaattagat acatataatt
     6661 aagcaaaagg aggctgatat gagccatcat cacgaagggt gttgtaaaca tgaaggccag
     6721 ccacgccatg agggctgctg caaaggtgag aagtcagaac acgagcactg cggacacggt
     6781 caccagcatg aacacggtca atgttgcggt ggtcgccacg gtcgcggcgg cggtcgtcgg
     6841 caacgtttct ttggtcacgg tgaattgcgt ctggtgattc tggatattct ctcgcgcgat
     6901 gacagccacg gttacgaatt gattaaagcg attgagaatc taacccaggg gaattacacc
     6961 ccaagcccgg gcgtcatcta cccaacgctg gattttctgc aggagcagtc gctgattacc
     7021 atccgcgaag aggaaggtgg taagaagcag attgcactga ccgaacaagg cgcgcagtgg
     7081 ctggaagaaa accgcgaaca ggtggagatg attgaagaac gcatcaaagc gcgttgcgtt
     7141 ggcgctgcgc tgcgccagaa cccgcaaatg aagcgagcgc tggataattt taaagcggtg
     7201 ctggatttac gcgtcaacca gagcgatatc actgatgcac aaataaaaaa gatcattgcg
     7261 gtgatcgacc gcgccgcttt tgatattacg caactggatt aatcaccgat tgccggatgc
     7321 ggcgtaccct acacaccccg taggcctgat aagatgcgcc agcatcgcat caggcattgt
     7381 gctccaaccg ccggatccgg aataccgatt aatgcagcac cgtcaccgcg tcttccagtc
     7441 ggctggcgcg gtgtttcacc atcgccgaca cctgcgcact ctcttccacc agctcggcat
     7501 ttttctgggt gatgaggttg agctcatcca ctgcacgggt caggctggaa agaccatcgg
     7561 cctgttccag cgttgaatgg ctaatctggg caatcaactg ggtgacgttt ttcacctgtg
     7621 ccacaatatc ttccatcgta cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct
     7681 tatcagcact ggcatcaatc agcttgcgaa tatcgttggc agcattggcg ctgcggctgg
     7741 ctaaatgacg catttccccc gccaccactg caaaaccttt gccctgttcg ccggctcgcg
     7801 ccgcttccac cgccgcattc agggccagaa tattggtctg aaacgcaata tcgttaatca
     7861 gcgaagtaat ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt gtcttgatca
     7921 ccgtggtcat cgcctccccg ccctgcactg ccgcattact ggcggtgatt gacagtttat
     7981 cggcagccga cgccgtggca gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg
     8041 ccaccgtttg ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc
     8101 ctttcgccag cgtctcactg ccgtttctga cactggacac ctagcttgag acatcgttaa
     8161 ttagccaacg gcacatcagg ccaagttgcc ctaccgcacg taatgtcagc cccagctcat
     8221 cgctgcgatt cagatgctca acactattac gttctccggt cgccaccttc agtgcctgac
     8281 gggcaacatt ttctatcggg cgcacaatct gccattcaaa acaggcgctt gccaacaata
     8341 ccactaacgc acagaggata tacgtcacca ccggggcagc aacaaaccaa agcatggccg
     8401 ccagcaagat aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag
     8461 gcagtttacc cagccagcct ttacgcacca ccaggccttt atgaatacgc ttactggtac
     8521 gtccggcgct caacgctttg tacagcggct ccaccgccgc gatctcttca tccgtcgccc
     8581 gggtacgaat cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg
     8641 cccgcaccca ataatggtca ccatttttgc ggcgattttt cacgatgccg ctccagggct
     8701 cccctttttt cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac
     8761 gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa
     8821 aagtgtcatt agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat
     8881 cgtccgccag cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct
     8941 gtgcaggtta tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat
     9001 ctttaaccga ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattcgt
     9061 tgatttaaaa tactttcact ctgttactat acgaaaacgt taattatctt gcccaaaaat
     9121 caggcaatta tagccctgaa aacgtgcatt tgcgcagcaa ttatcaaatc catacccgac
     9181 aaaaaccgtg caaaataaca acggatgtta acaaatagca ttaaatattg cacaaatgat
     9241 aaccacattt gtgtttatcc cgattttcgc gatcgcagcc ggagtggcgc aatccctgca
     9301 atacttaaat cggtatcatg tgatacgcga gcctccggag catattttga acaggttacc
     9361 ttcgagcgca tcggctttag cgtgcagcgc ccacgccctg aatctcattg aaaagcgaac
     9421 gctggatcat gaggagatga aagcacttaa ccgagaggtg attgaatact tcaaagagca
     9481 tgtcaatccg gggtttttag agtatcgcaa atctgttacc gccggcgggg attacggagc
     9541 cgtagagtgg caagcgggag gtttaaatac gcttgtcgac acccagggac aggagtttat
     9601 cgactgcctg ggaggttttg gaattttcaa cgtggggcac cgtaatccag ttgtggtttc
     9661 cgccgtacag aatcaacttg cgaaacaacc actgcacagc caggagctac tcgatccgtt
     9721 acgggctatg ctggcgaaaa cccttgctgc gctaacgcca ggtaaactga aatacagctt
     9781 cttctgtaat agcggcaccg agtccgttga agcggcgctg aagctggcga aggcttatca
     9841 gtcgccgcgc ggcaaattta cttttattgc caccagcggc gcgttccacg gtaaatcact
     9901 tggcgcgctg tcggccacgg cgaaatcgac cttccgcaaa ccgtttatgc cactgctgcc
     9961 agggttccgt catgtgccgt ttggcaatat cgaagccatg cgcacggctc ttaatgagtg
    10021 caaaaaaacc ggtgatgatg tggctgcggt gatcctcgaa ccgattcagg gcgaaggtgg
    10081 cgtaattctg ccgccgccgg gctatctcac cgccgtgcgt aagctgtgcg atgagttcgg
    10141 cgcactgatg atcctcgatg aagtacaaac gggcatgggg cgcacgggca agatgtttgc
    10201 ctgcgaacat gagaacgttc agccggacat tctgtgtctg gcaaaagccc tcggcggcgg
    10261 cgtgatgccg attggcgcaa ccatcgccac tgaagaggtg ttttcagttc tgttcgacaa
    10321 cccgttcctg cataccacca cctttggcgg caacccgctg gcctgtgcgg cggcgctggc
    10381 gaccatcaat gtgttgctgg agcagaactt accggctcag gctgaacaaa aaggcgatat
    10441 gttgctggac ggtttccgtc aactggcgcg ggaatatccc gatctggtac aggaagcgcg
    10501 tggtaaaggg atgttaatgg cgattgagtt tgttgataac gaaatcggct ataactttgc
    10561 cagcgagatg ttccgccagc gcgtactggt ggccggaacg ctcaataacg ccaaaacgat
    10621 ccgcattgaa ccgccactga cactgaccat tgaacagtgt gaactggtga tcaaagcggc
    10681 gcgtaaggcg ctggcggcca tgcgagtaag tgtcgaagaa gcgtaatatc aaatcggatg
    10741 gcgatacgac gtcgccatcc gatcttttta tacgacgcgt acgcccgcag gcatcatccg
    10801 ctccggcgtt aacagcacgc tttcactgcc gtcatcggtt tccgcacaca acagcatgca
    10861 ttctgacgtt tcaccgcgca ttttcgcttt ttgcagattg cataagacca ccaccgtttt
    10921 tcccatcagc tcttcttcgc tgtagtacgg caccaggctg gtcacggttt gcagcgtttt
    10981 ttcccccaca tcaacctgta cgatgtacag cttgtcggcg ttttcatggc gtttcacttc
    11041 cacaatcttt ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca cggtttccat
    11101 tgcttcctcc cttcgagtaa aattttacta aactatagca aaagtttttc tcaatcctgt
    11161 aggctaaaaa tggagaatgc aggcgtgatc acattcgtga gccgctgtgt taccgttaca
    11221 gcgtcaaaga aacgcgcttt atttactgaa aacaggtgac ccgataagca cttcctctac
    11281 aatggaagcg cacatcaggg aaagtaaaaa aggtaaacat ggcaacacta aaagacatcg
    11341 caatcgaagc tggcgtatcc ctggcgacag tatccagggt cttaaatgac gatccgacat
    11401 tgaatgtgaa agaagagacg aagcatcgca ttctcgagat cgccgaaaag ctggagtaca
    11461 agaccagtag cgcccgtaaa ctacagacag gcgcagtcaa ccaacaccat attctggcta
    11521 tctacagcta ccagcaggag ctggagatca acgatcctta ctatctggcg atccgccacg
    11581 gcattgaaac tcagtgcgaa aagctggcga tcgaactgac caactgttat gaacacaacg
    11641 gattaccaga cattaaaaac gtcaccggta ttttaattgt cggcaaaccc acgcccgccc
    11701 tgcgcgccgc cgcctgtgcg ttgaccgaca atatctgttt tatcgacttt cacgaaccca
    11761 gcagcggtta cgatgcggtg gatatcgatc tggcacgcat cagtaaagaa atcatcgact
    11821 tctatatcaa ccagggcgtt aatcgcattg gttttattgg cggtgaagat gagcctggca
    11881 aggcggatat tcgtgaggtc gcctttgcgg aatatggccg actgaaacaa gtggtacgcg
    11941 aagaggatat ctggcgcggc ggtttttcca gttcgtcggg ttatgaacta gcaaaacaga
    12001 tgctggcgcg ggaagactat ccgaaggcac tgtttgttgc ttccgattcc attgctatcg
    12061 gcgtactgcg ggcaattcat gaacgtggcc tgaacatccc acaggatatt tcgcttatca
    12121 gcgttaacga tatccccacc gcgcgattta cctttccgcc gctctccacc gtgcgcatcc
    12181 attccgaaat gatgggaagt cagggcgtaa acctggtgta tgaaaaagcc cgcgatggtc
    12241 gcgcgctgcc gctgttagtc ttcgttccca gcaaattaaa actgcgcggc acgacccgtt
    12301 aaatcccctt acacactgtc cggcagtcgt ttttgccgga cagtgctgcc gtttattttc
    12361 gtgatccagt taaagtaaat gcatttaccc gctacttttt agtaaaaatt ttactaaact
    12421 cccctgcaat tacacaaact accatcacca tgaatggttc cgatttctct ctaccgggag
    12481 gccctatgaa tcgctgggaa aacattcagc tcacccacga aaaccgactt gcgccgcgtg
    12541 cgtacttttt ttcatatgat tctgttgcac aggcgcgtac ctttgcccgc gaaaccagca
    12601 gcctgtttct gcccttaagc ggtcagtgga atttccattt ttttgaccac ccgctgcaag
    12661 tgcctgaagc cttcacctct gagttaatgg ctgactgggg gcatattacc gtccccgcca
    12721 tgtggcaaat ggaaggtcac ggcaaactgc aatataccga cgaaggtttt ccgttcccca
    12781 tcgatgtgcc gtttgttccc agcgataacc caaccggtgc ctatcaacgt attttcaccc
    12841 tcagtgacgg ctggcagggt aaacagacgc tgattaaatt tgacggcgtc gaaacctatt
    12901 ttgaagtcta cgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc ctgaccgcag
    12961 agtttgacat cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg cgcgtgatgc
    13021 agtgggcgga ctccacctac gtggaagacc aggatatgtg gtggtcggcg gggatcttcc
    13081 gcgatgttta tctggtcgga aaacacctaa cgcatattaa cgatttcacc gtgcgtaccg
    13141 actttgacga agcctattgc gatgccacgc tttccagcga agtggtgctg gaaaatctcg
    13201 ccgcctcccc tgtcgtcacg acgctggaat ataccctgtt tgatggcgaa cgcgtggtgc
    13261 acagcagcgc cattgatcat ttggcaattg aaaaactgac cagcgccagc tttgctttta
    13321 ctgtcgaaca gccccagcaa tggtcagcag aatcccctta tctttaccat ctggtcatga
    13381 cgctgaaaga cgccgacggc aacgttctgg aagtggtgcc acaacgcgtt ggcttccgtg
    13441 atatcaaagt gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg ctgcacggcg
    13501 tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt tggaatggat cgcgtcgaga
    13561 aagatctcca gttgatgaag cagcacaaca tcaactccgt gcgtaccgct cactacccga
    13621 acgatccgcg tttttacgaa ctgtgtgata tctacggcct gtttgtgatg gcggaaaccg
    13681 acgtcgaatc gcacggcttt gctaacgtcg gcgatatcag ccgtattacc gacgatccgc
    13741 agtgggaaaa agtctacgtc gagcgcattg ttcgccatat ccacgcgcag aaaaaccatc
    13801 cgtcgatcat catctggtcg ctgggcaatg aatccggcta tggctgtaac atccgcgcga
    13861 tgtaccacgc agcgaaggcg ctggatgaca cgcgactggt gcattacgaa gaagatcgcg
    13921 atgctgaagt ggtcgatatt atttccacca tgtacacccg cgtgccgctg atgaatgagt
    13981 ttggtgaata cccgcatccg aagccgcgca tcatctgtga atatgctcat gcgatgggga
    14041 acgggccggg cgggctgacg gagtaccaga acgtcttcta taagcacgat tgcattcagg
    14101 gacattatgt ctgggaatgg tgcgaccacg ggatccaggc gcaggatgac aacggcaatg
    14161 tctggtataa attcggcggc gactacggcg actatcccaa caactataac ttctgtcttg
    14221 atggtttgat ctattccgat cagacgccgg gaccaggcct gaaagagtac aaacaggtta
    14281 tcgcgccggt aaaaatccac gcgctggatc tgactcgcgg cgagctgaaa gtcgaaaata
    14341 aactgtggtt taccacgctt gatgactaca ccctgcacgc agaggtgcgc gccgaaggtg
    14401 aaacactcgc gacgcagcag attaaactgc gcgacgttgc gccgaacagc gaagccccct
    14461 tgcagatcac gctgccgcag ctggacgctc gcgaagcgtt ccttaacatt acggtgacca
    14521 aagattcccg cacccgctac agcgaagccg gacaccctat agccacttat cagttcccgc
    14581 tgaaggaaaa caccgcgcag ccagtgcctt tcgcaccgaa taacgcccgt ccgctgacgc
    14641 tggaagacga tcgtttgagc tgcaccgttc gcggctacaa cttcgcgatc accttctcaa
    14701 aaatgagtgg caaaccgaca tcctggcagg taaatggcga gtcgctgctg acccgcgagc
    14761 caaagatcaa cttcttcaag ccgatgatcg acaaccacaa gcaggagtac gaagggttgt
    14821 ggcagccgaa tcatttgcag atcatgcagg aacatctgcg cgactttgcc gtagaacaga
    14881 gcgatggtga agtgttgatc atcagccgca cagttattgc cccgccggtg tttgacttcg
    14941 ggatgcgctg tacctacatc tggcgcatcg ctgccgatgg ccaggttaac gtggcgcttt
    15001 ccggcgagcg ttacggcgac tatccgcaca tcattccgtg catcggtttc accatgggga
    15061 ttaacggcga atacgatcag gtggcgtatt acggtcgtgg accgggcgaa aactacgccg
    15121 acagccagca ggctaacatc atcgatatct ggcgcagcaa cgtcgatgcc atgttcgaga
    15181 actatccctt cccgcagaac aacggtaacc gtcagcatgt ccgctggacg gcactgacta
    15241 accgccacgg caacggtctg ctggtggttc cgcagcgccc aattaacttc agcgcctggc
    15301 actataccca ggaaaacatc cacgctgccc agcactgtaa cgagctgcag cgcagtgatg
    15361 acatcaccct gaacctcgac caccagctgc ttggcctcgg ctccaactcc tggggcagcg
    15421 aggtgctgga ctcttggcgc gtctggttcc gtgacttcag ctacggcttt acgttgctgc
    15481 cggtttctgg cggagaagct accgcgcaaa gcctggcgtc gtatgagttc ggcgcagggt
    15541 tcttttccac gaatttgcac agcgagaata agcaatgagg atcatcgata acttagaaca
    15601 gttccgccag atttacgcct ctggtaagaa gtggcaacgc tgcgttgaag cgattgaaaa
    15661 tatcgacaac attcagcctg gcgtcgccca ctccatcggt gactcattga cttaccgcgt
    15721 ggagacagac tccgcgaccg atgcgctatt taccgggcat cgacgctatt ttgaagtgca
    15781 ttactacctg caagggcagc aaaaaattga atatgcgccg aaagagacat tacaggtcgt
    15841 ggaatattat cgtgacgaaa ctgaccgcga atatttaaaa ggctgcggag aaaccgtaga
    15901 ggtccacgaa gggcaaatcg ttatttgcga tatccatgaa gcatatcggt ttatctgcaa
    15961 taacgcggtc aaaaaagtgg ttctcaaagt caccatcgaa gatggttatt tccataacaa
    16021 ataacaacta cggcggcaaa aggagtttgc cgccaccgct accctactca ttttcggaga
    16081 tgtgttatgt ctgataccaa acgtaataca atcggcaaat tcggcttgct ctcgctgact
    16141 tttgccgccg tttacagctt taacaacgtt atcaataata atattgagct tggactggcc
    16201 tcggcaccga tgtttttcct cgcgacgatt ttttatttta ttcccttctg tctgatcatc
    16261 gcagaatttg tttcgttaaa taaaaactca gaagccggtg tctacgcgtg ggtaaaaagt
    16321 tcgctgggcg gacgttgggc atttattact gcctatacct actggttcgt aaacctgttc
    16381 tttttcacct cgctgttgcc gcgcgttatt gcttatgctt cgtatgcctt cctcggctac
    16441 gaatatatta tgacgccggt tgccaccacc attatcagta tggtgctgtt cgccttctct
    16501 acctgggttt ccaccaacgg ggcgaaaatg ctggggccaa ttacctccgt cacttcaacg
    16561 ctgatgctgc tgttaacgct ctcctacatt ttactggcag gtacggcact ggttggcggc
    16621 gtacagcctg ccgatccgat caccgttgac gcgatgatcc cgaacttcaa ctgggcgttc
    16681 ctcggcgtta ccacctggat ctttatggcc gcaggtggcg cagagtccgt cgcggtgtac
    16741 gttaacgacg tcaaaggcgg ttcgaaatcg ttcgttaaag tgatcatcct cgccgggatt
    16801 tttatcggcg tactatattc cgtctcctcg gtgctgatta acgtcttcgt cagcagcaaa
    16861 gagttgaaat ttaccggcgg atcggtgcag gtattccacg gcatggcggc gtattttggt
    16921 ctaccggaag cgttaatgaa tcgctttgtc ggtctggtgt cctttaccgc aatgttcggt
    16981 tccctgctga tgtggaccgc aacgccagtg aaaattttct tctccgaaat cccggaaggc
    17041 atctttggta agaaaaccgt caaactgaac gaaaacggcg ttccggcgcg cgcagcgtgg
    17101 atccagttcc tgatcgtcat cccgctgatg attatcccga tgctcggttc caataccgtg
    17161 caggatctga tgaatactat tattaatatg accgccgcag cgtccatgct tccgccgtta
    17221 ttcatcatgc tggcttacct gaatttacgc gccaaattag atcacctgcc acgcgatttc
    17281 cgtatgggct cccgccgcac cggtattatc gttgtttcaa tgctgattgc gatatttgcc
    17341 gtagggtttg tcgcttcgac attcccgact ggcgcgaata ttctgaccat cattttttat
    17401 aacgtcggcg gtattgttat cttccttggc tttgcgtggt ggaaatacag taaatatata
    17461 aagggattaa cggctgaaga gcgccatatt gaagcaacgc cagccagcaa tgttgattaa
    17521 gcagaataat aaaaaatga
//
LOCUS       DAZUGK010000090        17521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZUGK010000090 DAZUGK010000000
VERSION     DAZUGK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.090"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..1184
                     /gene="arnB"
                     /locus_tag="ACHY5W_002614"
     CDS             45..1184
                     /gene="arnB"
                     /locus_tag="ACHY5W_002614"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFS4447492.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIAMTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1188..2156
                     /gene="arnC"
                     /locus_tag="ACHY5W_002615"
     CDS             1188..2156
                     /gene="arnC"
                     /locus_tag="ACHY5W_002615"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFS4447493.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2156..4138
                     /gene="arnA"
                     /locus_tag="ACHY5W_002616"
     CDS             2156..4138
                     /gene="arnA"
                     /locus_tag="ACHY5W_002616"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFS4447494.1"
                     /translation="MKTVVFAYHDMGCLGIEALLVAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVTRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEVAQRENEATCFGRRTPDDSFLEWYKPASVLH
                     NMVRAVADPWPGAFSYAGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIS
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4135..5025
                     /gene="arnD"
                     /locus_tag="ACHY5W_002617"
     CDS             4135..5025
                     /gene="arnD"
                     /locus_tag="ACHY5W_002617"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFS4447495.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNATSLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIALGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5025..6677
                     /gene="arnT"
                     /locus_tag="ACHY5W_002618"
     CDS             5025..6677
                     /gene="arnT"
                     /locus_tag="ACHY5W_002618"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFS4447496.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQSWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSIDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6674..7009
                     /gene="arnE"
                     /locus_tag="ACHY5W_002619"
     CDS             6674..7009
                     /gene="arnE"
                     /locus_tag="ACHY5W_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFS4447497.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIALWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7009..7395
                     /gene="arnF"
                     /locus_tag="ACHY5W_002620"
     CDS             7009..7395
                     /gene="arnF"
                     /locus_tag="ACHY5W_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFS4447498.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHY5W_002621"
     CDS             complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHY5W_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFS4447499.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCTAAGKTQ"
     gene            complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHY5W_002622"
     CDS             complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHY5W_002622"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFS4447500.1"
                     /translation="MIFSDWPWRHWRQMRGETIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RAVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQVLKEWVQRQ
                     Q"
     gene            complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHY5W_002623"
     CDS             complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHY5W_002623"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFS4447501.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLRLRL
                     DANRAWTLLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHY5W_002624"
     CDS             complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHY5W_002624"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFS4447502.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHY5W_002625"
     CDS             complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHY5W_002625"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFS4447503.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAIRVDGFDDVTDLLRKTLVSYNILNFWLAGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHY5W_002626"
     CDS             complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHY5W_002626"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFS4447504.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHY5W_002627"
     CDS             complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHY5W_002627"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFS4447505.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHY5W_002628"
     CDS             complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHY5W_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFS4447506.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15199..15660)
                     /locus_tag="ACHY5W_002629"
     CDS             complement(15199..15660)
                     /locus_tag="ACHY5W_002629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4447507.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15725..16642
                     /gene="rbn"
                     /locus_tag="ACHY5W_002630"
     CDS             15725..16642
                     /gene="rbn"
                     /locus_tag="ACHY5W_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFS4447508.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16830..>17521
                     /locus_tag="ACHY5W_002631"
     CDS             16830..>17521
                     /locus_tag="ACHY5W_002631"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFS4447509.1"
                     /translation="MIVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTAPDNSDDLLFKKLSSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNDNIKSDP
                     SWPKEVQLFPINT"
BASE COUNT         4129 a         4731 c         4421 g         4240 t
ORIGIN      
        1 agcaaatata agtggacagg tattcaatgg cggaaggaaa agcaatgtca gaatttttgc
       61 ctttttcgcg accagcaatg ggcgtggagg aactcgctgc agttaaagag gttctcgaat
      121 ctggttggat cacaaccggt ccgaagaatc acgcgcttga acaagccttt tgccagttga
      181 cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat atcacgctaa
      241 tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa
      301 ccctcaatat gatttccttg ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata
      361 cgctgatggt cacgcctgaa gctatcgagt cagccattac gccacgcact aaagccatca
      421 ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc attggcgaac
      481 gttacggcat cgcagttatc gaagatgcag cccatgccgt cggtacgtat tacaaagggc
      541 gacatattgg cgcaaaaggt accgctattt tttcgtttca cgccatcaaa aatattacct
      601 gtgcggaagg tggcctgatt gttactgata atgaaaacct tgcccgccag ctacggatgc
      661 tgaaatttca cggtctgggt gtcgatgcct atgacagaca aacctggggc cgtgcaccgc
      721 aggctgaagt cttaacgccg ggctataagt acaatctgac cgatattaac gccgcgattg
      781 ccatgacaca gttagccaaa ttagagcacc tcaacacccg tcggcgcgaa attgcccagc
      841 aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc gcctggccgc
      901 acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt ggtatcagtc
      961 gcgatgcgtt gatggaagcg ttaaaagaaa gaggtattgg taccgggtta catttccgcg
     1021 ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta ccgaataccg
     1081 aatggaatag cgaacgcatc tgttcgttgc cgctgttccc ggatatgact accgccgatg
     1141 ccgaccgcgt catcacagcc cttcagcaac tcgcaggaca ataagccatg tttgaaatcc
     1201 accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa agcttaccgg
     1261 aattaatcag gcgcaccacc acagcctgtg aatcactggg gaaagagtat gagatcctgc
     1321 tgattgatga cggcagtagc gataattccg cgcatatgct ggtcgaagcc tcacaagcgg
     1381 agaacagcca tattgtgtct attttgctta accgcaatta cgggcagcat tcagcgatta
     1441 tggcgggatt cagtcacgtt actggcgact taattattac cctcgatgcc gatctccaga
     1501 atccgccaga ggagatcccc cgtctggtgg caaaagccga tgaaggttac gacgtggtag
     1561 ggactgtacg ccagaaccgc caggacagct ggtttcgcaa aaccgcttcg aagatgatta
     1621 accggcttat tcagcgcacc accggcaaag cgatgggtga ctacggttgt atgctgcgcg
     1681 cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgcagc acctttatcc
     1741 cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtgcat catgccgagc
     1801 gtgagtatgg tgaatccaaa tacagcttta tgcgcctgat taatttgatg tacgacctgg
     1861 tgacctgcct taccacaacg ccgctacgta tgctgagcct gctcggcagc attattgcga
     1921 ttggaggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc ggaccacaat
     1981 gggcggcaga aggtgtcttt atgctatttg ccgtgctgtt tacttttatt ggcgctcagt
     2041 ttatcggcat gggattgctc ggtgaatata tcggcaggat ctacaccgat gtccgcgccc
     2101 gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat gaataatgaa
     2161 aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagccc tgctggttgc
     2221 cggttacgaa attagcgcca tttttaccca tactgataat cccggtgaaa aagcctttta
     2281 tggttcggtg gctcgtctgg cggcggaaag aggcattccg gtttatgcgc cggataacgt
     2341 taatcatccg ctgtgggtgg aacgcattgc ccaactctca ccagaggtga ttttctcttt
     2401 ttattatcgc catcttattt gcgacgagat tttgcagctc gctcccgcag gtgcatttaa
     2461 tctgcacggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt
     2521 gaacggtgaa acggaaacgg gcgttacatt gcaccgaatg gtgacacgtg ccgatgccgg
     2581 ggccattgtg gcgcaactgc gcgttgccat tgcgccagat gatatcgcca ttacgctgca
     2641 tcataaattg tgccatgctg cgcgccagct actggaacag acattacccg ccattaaaca
     2701 cggtaatatt ctggaagtcg cccagcgcga aaacgaagcc acctgttttg gtcgcagaac
     2761 gccggatgac agtttcctcg aatggtataa gccggcatcc gtactgcaca acatggtacg
     2821 tgccgttgcc gatccgtggc cgggtgcctt cagctatgct ggcaatcaga aattcaccgt
     2881 ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg tgatttctgt
     2941 tgcgccgttg ctgattgcct gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg
     3001 cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc
     3061 acgcttgaat agccagcctg cctgtgccgc ccgacgccgt acccgggtac tcatcctcgg
     3121 ggtaaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag atcattatga
     3181 agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc atccgcattt
     3241 tcactttgtc gaaggcgata tcagtattca ttccgaatgg attgagtatc acgtcaaaaa
     3301 atgtgatgtc gtcttgccgc tggtggcgat agccacgccg attgaatata cccgcaaccc
     3361 gctgcgcgta tttgaactcg attttgaaga gaatctgcgc attatccgct actgcgtgaa
     3421 gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa
     3481 atacttcgat gaggaccatt ctaatttaat cgtcggcccg gtgaataaac cacgctggat
     3541 ttattcggta tcaaaacaat tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg
     3601 tttacagttc accctcttcc gcccgtttaa ctggatggga ccacggctgg ataaccttaa
     3661 tgcagcgcga attggcagct cccgcgctat tacgcaactc attctcaatc tggtagaagg
     3721 ttcaccgatt aagctgattg atggcggaaa acaaaaacgc tgctttactg atatccggga
     3781 tggtatcgag gcgttgtacc gcattatcga aaatgcggga aatcgctgcg acggcgaaat
     3841 tatcaacatt ggcaatcctg agaacgaagc gagcattgag gaactgggcg agatgctgct
     3901 ggcgagcttc gaaaaacatc cgctgcgcca tcacttcccg ccgtttgcag gctttcgcgt
     3961 tgtcgaaagt agcagctact acggcaaagg atatcaggat gtagagcatc gtaaaccgag
     4021 catcagcaat gcccgccgct gcctggactg ggaaccaaaa attgatatgc aggaaaccat
     4081 cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac catcatgacc
     4141 aaagtcggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt
     4201 ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag cgtcggcccg
     4261 gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg gaagatgctg
     4321 cgctcaaacg cgacatcgct ttatggctgg gatattttac tggcaggtac ggcctggcca
     4381 ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa acatcacgaa
     4441 gtcggcctgc acgcctggga tcaccatgcc tggcaagccc atagcggtaa ctgggatcgg
     4501 caaacaatga tcgacgatat tgcactcggc cttcgcaccc tggaagagat tatcggtcaa
     4561 ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc agcaggtgat cgaagcaaaa
     4621 gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg
     4681 ctcgaatcag gaaaccctgg cactgcgcaa attccggtga ccttacccac ctgggatgaa
     4741 gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa ccgcatcctg
     4801 cgagataaag gcacgccggt ttataccatt catgcagaag ttgaaggctg cgcttatcag
     4861 cataattttg tggatctcct caaacgcgca gctcaggaag gcgtgacatt ttgcccttta
     4921 agcgaactgt tatcagagac gttgccgctc ggacaagtag ttcgcggaaa tattgccgga
     4981 cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt
     5041 accttatcgg cctcttcgcg tttattgcct gctattacct gttaccgatc agcacgcgtc
     5101 tgctatggca acccgatgaa acgcgttatg cggaaatcag tcgagaaatg ctggcatccg
     5161 gcgactggat tgtgccccat ctgttagggc tacgttattt cgaaaaaccc attgccggat
     5221 actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt gtgcgggcag
     5281 gcgttatctt tgcgaccctg ttaactgccg cgctggtgac ctggtttact ctgcgcttat
     5341 ggcgcgataa acgtctggct ctactcgcca cagtaattta tctctcattg tttattgtct
     5401 atgccatcgg cacttatgcc gtgctcgatc cgtttattgc cttctggctg gtggcgggaa
     5461 tgtgcagctt ctggctggca atgcaggcac agtcgtggaa aggcaaaagc gcaggatttt
     5521 tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc gcccttgccg
     5581 tgccggtatt aagcgtactg ccatgggtag cgacgcaaaa acgctggaaa gatctcttta
     5641 tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg ggactggcga
     5701 tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat attcaacgct
     5761 ttgcactgga tgatgcccaa catagagctc cgttctggta ctacgtgccg gtcatcattg
     5821 ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacgcaggc tggaaaaacc
     5881 gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg ctgtttttct
     5941 ccgtcgctaa aggtaaattg cccacctata ttctttcctg ctttgcacct ctggcaatgc
     6001 tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgttg cggattaatg
     6061 gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg gtctccccct
     6121 ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct
     6181 ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac gtcgaaaaga
     6241 cctggtcttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga ttttcaattc
     6301 ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca caagaatcac
     6361 ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt ctggcatgga
     6421 gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa tacggcctta
     6481 attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac tggcttaatc
     6541 aacatcgtca ggaggggatt attacactcg tgctttcgat tgaccgcgat gaagatatca
     6601 acagtctcgc cattccgccc gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc
     6661 agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta gcgttgccgg
     6721 gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc gcaaacatat
     6781 cgcgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc tctggctgct
     6841 ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga attttgtctg
     6901 ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc actggtgtgg
     6961 ggtggcgttc attattggtg gcattgtgat cctcgggagt acggtgtaat gggcctgatg
     7021 tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg ttttgcggcg
     7081 agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt agcttttggg
     7141 ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt tttttgttgg
     7201 tataaaacac tacataaact tgcgctaagc aaagcctatg ccttgctcag tatgagttat
     7261 gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt ttcgctgaaa
     7321 gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct gcccacgaca
     7381 aaacaacgtt actgagtttt ccctgccgct gtacactgcc gttcccactc atcggggcta
     7441 taacagcttg cagagaggac tttcaccgta tgcagctttt cacgattaat acaataaagc
     7501 gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact cttcacttcg
     7561 gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct gttatcttgt
     7621 ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt gataagagtt
     7681 ttcacatcaa tttacagatt aacagataaa cctattatca acggcaatgg aatggcagaa
     7741 atgagaagtg aaacggaggg ttaattattg ctgacgttgc acccactctt ttagcacctg
     7801 acgtgaaatt ttaataccgc cgtttttcag ttcaggcggc aacgttaacc aacgcaccgg
     7861 ttgctgaaaa cgggccagct tatctttcac ccattcacta agatcaacgc tctcgtggtc
     7921 atactccatc accgccaccg ctcgatgacc aaactccttg tcggcaacgg ggacgataaa
     7981 cacctgcaaa accgcaggat gtgcagcaat tacgcgctcg acttcttccg gctgaatacc
     8041 ctctccgcca ctgaagaata gattgtctaa acgtccgaca atggtcagct tgccattatg
     8101 catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg aaaccagttg
     8161 cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt cattattaac
     8221 gattttcact tcccgacccg gcagcggcga accaacgtct gccaggccgt cggcttcttt
     8281 cgcacacacc gtggaggcaa actcggtcag accatagccg caaaagcaac gaatcccctg
     8341 ctcgcgtgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta acaccgcttt
     8401 cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca ccagtgaagc
     8461 gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg tcatccgcgc
     8521 accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca gcggtaaaga
     8581 gagcaaccag tcatcgtgat cgccaaacgg aatcaacgat aacacccctt cggcactggc
     8641 aagatgggcc tgataagtat ggacagcggc tttcggcaac ccggtagaac ccgaggtcaa
     8701 cgtcattgag cacagacgcg tcggctgcca cgtagcgaca tgtgcgcctt caacccgctg
     8761 aatgtgcagc gacgttaatg ccggaaacgt gttttcccca tccggcacca gcgcaaattg
     8821 cagcgtcaga ttgggcagca attcttcaag caacggttgc ggcagctgag ggttcacggg
     8881 caacacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt
     8941 ccacgcccgc aacatcacgc cgctgccctc aaccacaccc tgcaccgcaa aaccggaggc
     9001 taattcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa
     9061 ggcgatggtt tctccccgca tttgccgcca gtgacgccac ggccagtcag agaagatcat
     9121 aacaaccgct ccagtgcatc aacttccacg acaggcagcg tgctacccgg ccagcgacgt
     9181 acctgctgcg cctgcatcag atccagcgtg tccagccctg gaatggtgtc cggcgttaac
     9241 caggcggcaa tccgcgccag ttgcgttaag cctaagctcg attcaatgga agaactgatc
     9301 accgccgtca gccccagcgc gtgcgccgcc tgtacctgct cgcgtacttt ttccagactg
     9361 cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac aaaggcaaaa
     9421 tccggctcgc gcaggctttc atcccaggca atggcaatgc cggtttcacg ggcaaacgct
     9481 cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtc gcgataatcc
     9541 gggttaacgt atttggcaaa ctgctgacct ttcagcagtg tccaggcgcg atttgcgtca
     9601 agacgcaaac gcagatccgg aattgcctcc agcaacagat tcaccaccat gccgtcgcgc
     9661 accgcttcgt acaatcccac tttgaccttc gccactttct cgcctggcat atctgcaagt
     9721 ttgaggatca gatcgtccgg atcgccatta cacagcggtg ccgcacggta gttggctgct
     9781 tgcggcaacg tatctgtcag ttctgccaat gcacagctta cgccaaaggc cacggaaggc
     9841 atctgcggta gctcgcaatc gcctgccagc cagttattta cccaggcaag cagcacactt
     9901 tgcgcctctt cccaggtttc ctgactgaag cccggcagtg gggagatctc cccccaccct
     9961 tcgcgctcgc cttcacgcag gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg
    10021 cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc gctacgcatt
    10081 acggattccg tttgaatttg ctgaagtcag gctgacgttt ctggttgaag gcgttgcgac
    10141 cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc agctcctgca
    10201 gccccgcctg cccgtcacag tcggcgttca gcgcagcttt caggcaacgc agcgccatcg
    10261 ggctgttttg cagcatttca cggcaccaac ggacggtttc tttttccaga tccgccagcg
    10321 gtaccacggt gttgacaagg cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca
    10381 ggaaccagat ttcacgcgct tttttctgcc cgacgatacg agccatgtag gaagcgcccc
    10441 agccgccgtc gaaggaaccg actttcgggc cagtctgacc aaagatggca ttatctgccg
    10501 cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc gccgatggag tagccagcca
    10561 ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc agcacgttca
    10621 ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact ttctggtcac
    10681 caccggagca gaacgcttta tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt
    10741 cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga cggaaggcat
    10801 tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat ttttcataac
    10861 gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa agcattgctt
    10921 catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg ccagacttgc
    10981 gattacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca
    11041 gtcggcagcc agttccgccg ccagggcgcg gaatttgctg tcacgttcac cacataaata
    11101 ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcaggctgaa cggcgagaga
    11161 agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca gcgccaccag
    11221 ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc agtcggtaaa
    11281 caccgccgtt aacggttctg agcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg
    11341 ttccgcacgt tgttcagcat tttgcagccc cggatgccca ccttcgacaa taaccccaca
    11401 aagccccgcc agcccctggc aagccgccat catcgccacc cgtccaccaa gtgagtatcc
    11461 cgccaaccag aagttaagga tgttgtaact aaccagggtt ttacgcagta agtcagtgac
    11521 atcatcaaat ccatcgactc taatcgccgc cgaaccaccg tgacctggga gatcaacata
    11581 caaccgtgag tagtcggcaa acgcctcgcc tacttcttgc cattcgtggc aatcgccgga
    11641 aaaaccgtgc aaaaacacca gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg
    11701 caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg
    11761 tcgttaacca ccatttcaat caccgtggtg gttggcgtgc gccaggcgtc agcaagcgcc
    11821 gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg
    11881 tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cgcgctcgct ttgcggcgtt
    11941 ggcaacagcg agaaaatttg cccgccgttg ttgttcacca caattaatac cagcggcgcg
    12001 gaaacctggc gcaataacgc cagcgcattc aggtcgtaaa gtgcggagag atcgcccaca
    12061 atcgccagcg tcggtttgcc gcttgcccgc tgaacgccag cggcagtcga gagcagcccg
    12121 tcgataccgc tggccccacg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc
    12181 gcatcaatca gacgtaccac caggctgtta ccgacaaaca attgcccctg ttcaggcaga
    12241 tagtcgcaga tgcgatgcgc cagttgtgct tcgccaaacg catcgcgacg ggcaataacc
    12301 gcctgcattg cctgttccgc caggcgcgga atttcaacgc accagggctg gcgtttttct
    12361 gccggatgca gttccagcca gtcggcaata ttggcaatta accgacgccc gcgatggtgc
    12421 gccggatcaa gccgcccttc gatgtcatcc acaatccagt actcttcggg ttcacagctt
    12481 gcctgccatt gcaggagccg tttgcccgtc aggctgcttc ccagttgcac cacaatttgc
    12541 gcctgttgca gttcgctggt cgctttggca ttgcctaacc agagatcggc acacggcagc
    12601 ggctgtccgg tttgcgacag cacgtcgcca atcagcggcc agccaagagt ttgcgcccac
    12661 agtgcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgcttt
    12721 tgccgccaga agaaccagtc gcgctgtttt tcgctctcaa gacgaggcgc ttcacgcagc
    12781 cacggcttat catcctgcca ccagtcaccc agacgctgtt gccagctaag cccagtgtcg
    12841 tccatctcgc catacagcgg ttcagcaaac gggcagttga tatggacccc accagcatga
    12901 agcgtaccga gagcatggtc gatggtagaa accagccaac gtgcggggat atcctgggtc
    12961 gggcgcggca gtgaaatact gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc
    13021 gcctgattcg cgccgcagtc aattagctcc ggcgggcgat cggcggttaa gagaatcagt
    13081 ttttctccgg ttaacccggc ttcaatcagt gccggataga gatttgccac cgccgtaccg
    13141 gaggtgacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc
    13201 cccagtccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct
    13261 aacgttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtcttac gccgtgacgc
    13321 gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt
    13381 ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg
    13441 aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca gccctgccgc
    13501 tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc
    13561 agcatataat cgcacgacat tgccgctaat ttttgctgag cgcagggaaa cgcagaattc
    13621 gctttgttgt aatgagagat agcccgcaga accagcgtac cattctcggg tgaacggttc
    13681 gtgacgggcg ataaactgtc gcgccagatc gcgcggtaaa ccagcaactg ctgccgttgg
    13741 ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc agatacagcg
    13801 gcgaagatgc tgtactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg
    13861 ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc agcatgttct cgcgctggtt
    13921 tttatcatcc gccatcagcc actctcctaa ctgctgcgcc tgcttatcat caggatgatt
    13981 tgctactgtt cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg
    14041 ttccggtgaa gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca
    14101 attgagattc agtcgacgac tggccgccat catcgccgcc gcattgaccg gacttgcgaa
    14161 atgcaggtca gttgcccgag caagcaccac tttgtcgagc tcaccttcgg cgatggtttt
    14221 cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt
    14281 agtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattctct
    14341 tgccttaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc
    14401 tttaccgcca cagcgtcgcc attccaggcg tggtaaaagt aaattgccct gcgacgggtc
    14461 aaacgcattc agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg
    14521 ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc
    14581 accattacgt tgttgccagt agaattgcgg gtacgtttgc tgactggcca gccagctcaa
    14641 ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc
    14701 cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac
    14761 gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta
    14821 ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccaacggc
    14881 cgcacccaca ccaattccct gccagggttt ttcgtggacg tagtcatcag cacgataaac
    14941 agcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc
    15001 atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc
    15061 agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg
    15121 tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact
    15181 atagacaagg gttattgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat
    15241 accatcttct tcatagatct ccgtcacagg gataaaaccg aaactctggt agaagttttg
    15301 cagatgcgcc tgcgccccga ggtagacagg cttatcaggc cagtgatgcg tgcaagtgtc
    15361 gagggtttta ctcatcagtt gctgccccac tttttcgcca cgcaacgctt cactgacaat
    15421 cacccgacca ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatatgc
    15481 caccagttca tcatttttcc aaccgagaat atggcgatta tcccccgtca aatcatcacc
    15541 atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa
    15601 taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat
    15661 aatgctctcc ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga
    15721 actcatggaa ttaatttttt taggtacttc ggccggtgtg ccaacccgca cgcgcaatgt
    15781 cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt tgtttgactg
    15841 cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa aactggacaa
    15901 gatttttatc agtcaccttc atggcgatca tctttttggt ttacccggct tgctgtgcag
    15961 tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga
    16021 atttgtggag accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt
    16081 cgaaattggc gctggcgaaa ttctcgacga tggcctgcgc aaagtgaccg cttatccaat
    16141 ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt
    16201 aaatgcccag gcattaaaag ctgctggcgt gccgcctggc ccactgtttc aggaattaaa
    16261 agcgggcaaa acaatcacgc tggaagatgg aaggcagatt aacggcgcag attacttagc
    16321 tgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc
    16381 acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat
    16441 ggaagccaaa gccaatagtc gcggtcatag ctctacacgc caggctgcga cactggcccg
    16501 tgaggctgga gtcggtaagc taatcattac ccacgtcagc tcgcgctatg atgacaaagg
    16561 ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tagcgaatga
    16621 tttcaccgtg tttaacgttt aactctgctt attattaaaa acagggcgaa acttgccctg
    16681 ttatcgcaac ccgcgataca tatatccacc cacacgggca tattgagcga tttttgatat
    16741 ttattcatct cattacgcac tatattcaga gcaataagtt gcctcttttt atcttcattc
    16801 agcacaacct ttgcaactaa aaggagcata tgatagttac agttgtcagc aattattgtc
    16861 aattatctca aacccaactc agtcagacat ttgcagaaaa atttactgtg accgaggaat
    16921 tactgcagtc tttaaaaaaa acagcgttat ccggagatga agaaagtatt gagttactgc
    16981 ataacattgc gttaggttat gatgaatttg gaaaaaaagc tgaagatatt ctttaccata
    17041 ttgttagaaa cccaacaaat gagaccctat cgattatccg acttattaaa aatgcctgtt
    17101 taaaattata taatttggca cataccgcaa ccaattccca ccttaaacca actgctccgg
    17161 ataattcaga tgatctcctg ttcaaaaagc tatcctcccc ttcgaaatta atgacaatta
    17221 tcggcgatga aattcctctt atatctgaaa aacagtcgct ttcgaaggta cttttaaatg
    17281 atgagaataa tgaactgagt gatggtacaa acttctggga taaaaatcgt caattaacca
    17341 cagatgaaat agattgctat cttcagaaga tcgccgctaa tgcaaaaaat actcaagtca
    17401 attatcctac tggtctctac gtcccctact ccaccagaac tcatctggaa gacgctctca
    17461 atgataatat taagagcgat ccatcatggc cgaaagaagt ccagttattc cccataaata
    17521 c
//
LOCUS       DAZUGK010000091        17449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZUGK010000091 DAZUGK010000000
VERSION     DAZUGK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.091"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(95..826)
                     /gene="rlmB"
                     /locus_tag="ACHY5W_002632"
     CDS             complement(95..826)
                     /gene="rlmB"
                     /locus_tag="ACHY5W_002632"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4447510.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1007..3448)
                     /gene="rnr"
                     /locus_tag="ACHY5W_002633"
     CDS             complement(1007..3448)
                     /gene="rnr"
                     /locus_tag="ACHY5W_002633"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4447511.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3487..3912)
                     /gene="nsrR"
                     /locus_tag="ACHY5W_002634"
     CDS             complement(3487..3912)
                     /gene="nsrR"
                     /locus_tag="ACHY5W_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFS4447512.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4117..5415)
                     /gene="purA"
                     /locus_tag="ACHY5W_002635"
     CDS             complement(4117..5415)
                     /gene="purA"
                     /locus_tag="ACHY5W_002635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4447513.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5519..5716)
                     /gene="yjeT"
                     /locus_tag="ACHY5W_002636"
     CDS             complement(5519..5716)
                     /gene="yjeT"
                     /locus_tag="ACHY5W_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFS4447514.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5798..6802)
                     /gene="hflC"
                     /locus_tag="ACHY5W_002637"
     CDS             complement(5798..6802)
                     /gene="hflC"
                     /locus_tag="ACHY5W_002637"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFS4447515.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6805..8064)
                     /gene="hflK"
                     /locus_tag="ACHY5W_002638"
     CDS             complement(6805..8064)
                     /gene="hflK"
                     /locus_tag="ACHY5W_002638"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFS4447516.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8150..9430)
                     /gene="hflX"
                     /locus_tag="ACHY5W_002639"
     CDS             complement(8150..9430)
                     /gene="hflX"
                     /locus_tag="ACHY5W_002639"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFS4447517.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9507..9815)
                     /gene="hfq"
                     /locus_tag="ACHY5W_002640"
     CDS             complement(9507..9815)
                     /gene="hfq"
                     /locus_tag="ACHY5W_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFS4447518.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9901..10851)
                     /gene="miaA"
                     /locus_tag="ACHY5W_002641"
     CDS             complement(9901..10851)
                     /gene="miaA"
                     /locus_tag="ACHY5W_002641"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4447519.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10844..12691)
                     /gene="mutL"
                     /locus_tag="ACHY5W_002642"
     CDS             complement(10844..12691)
                     /gene="mutL"
                     /locus_tag="ACHY5W_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4447520.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLSLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12701..14035)
                     /gene="amiB"
                     /locus_tag="ACHY5W_002643"
     CDS             complement(12701..14035)
                     /gene="amiB"
                     /locus_tag="ACHY5W_002643"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFS4447521.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPVVAKRVETPTVVAPRVSEPARNPF
                     KTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVT
                     IAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRS
                     ATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFG
                     HSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEER
                     LLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14054..14515)
                     /gene="tsaE"
                     /locus_tag="ACHY5W_002644"
     CDS             complement(14054..14515)
                     /gene="tsaE"
                     /locus_tag="ACHY5W_002644"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4447522.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14487..16034)
                     /gene="nnr"
                     /locus_tag="ACHY5W_002645"
     CDS             complement(14487..16034)
                     /gene="nnr"
                     /locus_tag="ACHY5W_002645"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFS4447523.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRAAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVALDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLNESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16033..17172
                     /gene="queG"
                     /locus_tag="ACHY5W_002646"
     CDS             16033..17172
                     /gene="queG"
                     /locus_tag="ACHY5W_002646"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4447524.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17155..17208)
                     /locus_tag="ACHY5W_002647"
     CDS             complement(17155..17208)
                     /locus_tag="ACHY5W_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447525.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3973 a         4976 c         4377 g         4123 t
ORIGIN      
        1 taataaaatg aatatttgca atacgtaatt atcttaccag ctatagacaa aaaaaaccat
       61 ccaaatctgg atggcttttc ataattcaga ggaactagct gcgctgacga accgcttcaa
      121 ataagcaaat tccggttgca accgaaacgt tcagagaaga aacacttcct gccattggga
      181 tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca ccttccgcgc
      241 ccatcaccag cgccaggcgt ccggtcattt tgctctgata gagtgtatga tccgcctcgc
      301 ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg cgcgcaaggt
      361 tagtcacccg aatcagtgga acgctttctg ccgcgccgca ggctactttt ttcgccgtgg
      421 cgttgagctg tgcggagcga tctttcggca caatcaccgc gtgaacgcca gcggcgtccg
      481 cgctacgcag gcacgcgccg aggttgtgcg gatcggttac accgtcgagg atcagcagga
      541 acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac tgacgtcctg
      601 gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg tcgagatatt
      661 ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc agcggtaaca
      721 gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca ggggcgcgct
      781 ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat gtactcgttg
      841 tttacgtttt gcctgatgca ctacgtttat ccggtctaca tgatccctgc aatatattga
      901 atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg
      961 cattttgtca gcaatctaac cctcttctgt ttgaagaggg taatgatcac tctgccactt
     1021 ttttcttcgc cgcacgcttc gctttggtcg ctgcagctat tttctgcgtt ttcgccgatg
     1081 gctttttcgc ttttctcgcg tctttcttcg ctgctttcgg cttcgttttt ttctcaccgc
     1141 ggaaggcgct gtctggctca aagtttacct ttttaccgac ctgacgacgc ttgccgcctt
     1201 ttttacctgc atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg
     1261 cgcgttcgct ggagatcagg ctaaagtcga ttttgcgctc gtccatatta accgcttcga
     1321 cgcgaacttc cacgcgatcg cccaggcgat aagtctggcc gctggattca cccatcaggc
     1381 gttgccctac ctggtcaaag cgatagtagt cattgtccag cgaagagaca tggaccagcc
     1441 catcaatgaa caagtcgtcc agacggacga agaagccaaa gccagtgacg ctggaaatta
     1501 cgcctttaaa gacgttacct acctggtcga gcatgaagtc acacttcagc cagtcagcca
     1561 catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga cccagttgca
     1621 gcatctcttc catcgaataa tggtagccac cggtttcagt ggtgttgccc tgatgcccct
     1681 gctctttcgc caacagatac ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa
     1741 tcggcgaagt aaagtgcgca taggactgca atgccaggcc aaagtgacca cggttttccg
     1801 gatcgtaaat cgcctgtttc atcgagcgca gcagcatggt ttgcagcatt tctgcgtcag
     1861 gacgatcggc aacagactcc agcaactctg catagtcgcg cggttccggc ttgttaccgc
     1921 ccggcaattc cagccccagt tccgccagca ctgaacggaa agaggtaatc gcttcggtgc
     1981 tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc tttctcaacg aaacgcgccg
     2041 ccgagatatt cgccagaatc atgcactctt caattaactt gtgcgcgtcg ttacgctggg
     2101 tctgttcgat acgctcaata cggcgttcag cgttgaaaat gaacttcgct tcctcgctct
     2161 caaatgagat cccgccgcgt tcttcacggg ctttatccag cactttatag aggttatgca
     2221 actcttcgag atgcttaacc agcggggcgt actgctcgcg cagatcctga tcgccctgca
     2281 gaatatgcca gactttggtg taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga
     2341 atttgtagcc agtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc atacacaggc
     2401 ggtctacctg cgggttgagc gaacacaggc cgttagagag cacttccggc agcatcggga
     2461 taacctgcga agggaagtac accgacgtgc cacggttacg cgcttctctg tccagcggcg
     2521 ttggcggacg cacatagtag ctgacgtcag caatcgcgac ccataaacgc cagccgccgc
     2581 cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg
     2641 taaccagcgg taaatcgcgc agatcaacac ggcccgcttt tgcttcttcc ggcacttctt
     2701 ctttcagccc ggcaacctgt tgctcaacag cctgcggcca gatgtacgga atttcatggg
     2761 tacgcagagc gatatcaacc gccatgccgg tgcccatatt gtcgcccagc acttcgacga
     2821 ttttacccac cgctttggta cggcgagtcg ggcgttgggt cagttcaaca accaccacaa
     2881 agcccatccg cgcgcccatg atctgatcgg gcgggattaa gatatcgaag ctcagacgac
     2941 tatcgtcagg aaccacaaag ccgacgcccg cttcggtaaa gtagcgacca acaatctggc
     3001 tggtttttgg caccagtacg cggacaatac gcgcttcacg acgaccttta cggtcagcgc
     3061 ccagcggctg cgccagcacc tgatcgccat gaatgcaggt tttcatctgc tcgctggaga
     3121 gatacaaatc atctttacgc ccttcaaccc gcagaaagcc gtagccatca cggtggccaa
     3181 taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc atagcactga cgacgagtaa
     3241 agaccagttg accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct
     3301 cttcgccttc aatgtgcagt tctaccgcca gctcatcacg gctggccggt ttttcacgtt
     3361 tggttaaatg ttcgaggata aattcccgac tagggatggg attcgcgtat ttttcagctt
     3421 cgcgttcctg gaaaggatct tgtgacatct cggttcctcc gttgtcatct ctgatgaaga
     3481 ttttcgtcac tccaccagca ataatttata aagcggttga ttctcttcaa ccaaatcggc
     3541 aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt
     3601 caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca gcgataaggg
     3661 ctccagctcg cgcaccacat caccaatacg tatcgcactc gccggtttac ccaggcgaat
     3721 gccgccattt tttccacgaa cagcagtcac gtagccggca cgactaagtt gattgattat
     3781 tttgaccata tgattacggg agacgccgta gacgtcagtc acttcagaaa tactggtcat
     3841 ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat cagtgaaact
     3901 cgttaactgc acatcaacct caaaagggaa atcgggaaaa aatacattta tattgatgat
     3961 aaaccagcca caagctgtgt cgctaattta tttcagttcg gggaaggaaa aaagcgagga
     4021 tttaacactg tgtcggatag cgtaatggct taaccgacac agcaatgtat cacactgtta
     4081 gcccggcagg caaaatatct gccgggcgta ccagaattac gcgtcgaacg ggtcgcgcag
     4141 aatcatggtt tcagtacgat ccggaccggt tgagatgata tcgatcggca caccggtcag
     4201 ctcttcaata cgtttgatgt agttcagcgc cgcctgcggc agaccgctac gatctttcac
     4261 gccgaaggtg gattcagacc agcccggcat ggtttcgtaa atcggctcta cacctttcca
     4321 gtcgtcagct gccagcggag tggtagtcac ttcacgacca tccggcatac ggtaagccac
     4381 gcagagttta acctctttca ggccatccag aacgtccagt ttagtcaggc agaagccaga
     4441 cagggagttc aactgtaccg cacgacgaac ggcaacggtg tccagccagc cggtacgacg
     4501 acgacgcccc gtagttgcgc cgaattcgtt accctgcttg cagaggaact cgccagtttc
     4561 atcaaacagt tcagtcggga atggacctgc acccacacga gtggagtaag ctttgaggat
     4621 acccagaacg taatcaacat aacgcgggcc caggccggaa ccggtcgcca cgccaccagc
     4681 agtggtgttg gaagaagtta cgtacggata agtaccgtgg tcgatatcca gcagcgtacc
     4741 ctgcgcacct tcgaacatga cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga
     4801 aacgtcaacc accatagaag tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt
     4861 ctggtaatca accgcttcag ctttgtagta gttaaccaac tggaagttgt gatattccat
     4921 cacttctttc agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc
     4981 gcgacgtgcc actttatctt cataagcagg cccgatacca cgaccggtgg tgccgatcgc
     5041 tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca acgtgataat caaggatcag
     5101 cggacatgct tcagacagca gcagacgctc acgaacgggg atgccacggt cttccagttc
     5161 tttcatctct ttcatcagcg cggccggaga cagcacaaca ccgttaccga tgatgctggt
     5221 tacattctcg cggagaatac ctgatggaat aagatggaga acggtttttt caccgttgat
     5281 tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc
     5341 agtcagaaga tcgacgatct tacctttacc ttcgtcaccc cattgggtgc ccagtacgac
     5401 gacgttgtta cccatttttc aaaatcaccg tttgcttaaa aatggattct accatcgctt
     5461 tttcagagtt acagcacttt ttgcactcaa aaatgacctc gatcagtctg ttttttgttc
     5521 agccaatcgt tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc
     5581 caaaacgacg taaaatatta tcgggcaaat tggtcatcgc agagatcatc ttcttccatg
     5641 ccttcgggta aagcatcggc cctaaacctt ccagtaccaa aaccagggca agcgccagcc
     5701 agattgtcga attcattcat tatccttata gaaaaagaaa accaccgaca tccctgagga
     5761 tgcggtggct ttattgacct gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg
     5821 tcttcatgta gcggaagaaa tcgctatccg ggctcatgac catcacgtcc tgattgccag
     5881 agaagctgtt ctcataagca cgcaggctac ggatgaatgc gtagaagtcc ggatctttac
     5941 tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc ataatgcggc
     6001 cctgacgctc agcttctgcc agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt
     6061 tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc
     6121 gcatacggtt gtagatcgct tcagacactt cggtcggcag gttgatctgc ttgatacgca
     6181 catcgacaac ttcaataccc agcgccgcca tactgttcgg gttgatgacc ggaactttgc
     6241 ccttcgtctc agccgttacg cgctctgccg cttcggcaat ggcgttatct gccgccgggg
     6301 tagtaacttc atcttctgta cccgcagaac cggagttcag cgcgtcacgt acttcgaggg
     6361 tcagacgacc acgggaatca gtgacgatat ctttcacgtc caggcgacca atttcagaac
     6421 gcagacggtc agagaactta cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac
     6481 ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa gagtcgacga
     6541 tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac
     6601 gtgcgtcgag cattttcacc gtttcaatga acggtatctt gaaatgcaga cccggctcat
     6661 aaaccagagg tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc
     6721 gctcaccttc tttgacgaca aagacagaca tgtaaagcac taccagcacg atgatgataa
     6781 tcgcgataac tgacttacgc atcgttattc cccctgacgc tggtagtcgt tacgctgcgc
     6841 gttggcgcgg cgttggtcca taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc
     6901 actggttgtg gaggaagagg ctggcggcag acgcagcaga ttgctggcac cgttatcgct
     6961 cttcgccgca ggggcgttac cacctttcag catctggtct aacggcagaa ccatcaggtt
     7021 gccacctttg tcgttaacca gcactttgcg ggtgttaccc aacacttttt ccatcgtctc
     7081 gatatacaga cgctcgcgag taatttccgg cgcggcttta tattccggca gaagtttagc
     7141 aaagcgcgcc acttcaccct gagcttccag gatggtctgg gccttgtacg cacgcgcctc
     7201 ttcgaggata cgttgcgcct gaccgttcgc acgcggctga acttcgttgg tatacgcttc
     7261 tgcttcacga atgtattgct gttcgttttc acgcgcggca atcgcatcgt caaacgccgc
     7321 ttttacttct tccggcggac gagctgcctg gaagttgacg tccagcagag tgatgcccat
     7381 gtcatacgga cgaatcgtct cttccagttc gcgctgagta tcgctacgaa tcacggtacg
     7441 accttccgtc agaatgcggt ccatggtgta tttaccgata actccacgca gggcgctgtc
     7501 ggtagcctga cgcaggctgt catccgggct ggtcacgcta tacagatatt tttccggatt
     7561 ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg ttctcgtccg acgtcagcat
     7621 cacaccagaa gcggccagtt cacgcacggc ttccacgttc accggtttga cttcgtcgat
     7681 aaacgtcggt ttccagttca gacccggctc aaccagatgg ctgaatttac caaagcgtgt
     7741 taccacgccg cgttcggctt ctttaatggt atagaaacca ctggccgccc agataatgac
     7801 aatcgctgcc gctgcgatgg taacgacacg accgccaagc tgcgggcgcg ggccttgcga
     7861 tgaactgcca ccgccagatc cggtgccttt accgccgccc agaccaccga gctttttgct
     7921 cagtttgcgg aagatatcat ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc
     7981 atttccctca gagttgccgc caggtttgct gcttccccac gggtcgcggt cttgtccgtt
     8041 attaccgggc tgattccacg ccatgtttgt gctccatatt tgttatgcgg tgatccctgt
     8101 tgtctttcga cgccagagga tatgactcca cgcttcagac gctacgccgt tagatcaggt
     8161 aatcgatcaa cgccggttct tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa
     8221 cttgcagact tacgctgccg tcctcctcca tccactcttt ttctattgcc tgaagctgat
     8281 aaaaacgact tctcagacgc ccttcctgcg gtggcagacg caatgtatgc tgcgccacct
     8341 cgccggaaag ccgctccgtc aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg
     8401 cggaaagcca gacacggatc ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat
     8461 cttccagcat atcgatcttg ttcatcacca gcagggttgg gatctcgtga gcgtcgatct
     8521 cttcaagaac cgtattcacc gcttcgatgt tttcttgtac acgcacatcc gccgcatcaa
     8581 tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca
     8641 ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc tgccaacacg gtttcaccga
     8701 catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg
     8761 cgtagacccg cgcttcggtg atgcgattga aaagggtaga tttaccggcg ttggtatatc
     8821 ccaccagcga aacagtagga acgtcggctt tgatacgcga ttgccgcccc tgctcacgct
     8881 gcttttcaac tctttccagg cgcgactgta tctgcacgat gcgattacgc aacaaacgac
     8941 ggtcggtttc gagctgggtt tcacccggac cacgcaaacc tatcccgcct ttctgtcttt
     9001 caaggtgggt ccagccacgc accaggcgcg tagccagatg gcgcagctgc gccagctcaa
     9061 cctgcaactt accctcatgg gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg
     9121 tgcggtcgat aacacgacac tcgcacaaac gctccaggtt acgctcttgc gccgggctca
     9181 gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa
     9241 ctgctttacc ttcacctaca aaatactttg ggtgcggcgc tttacggcta ccggtaatca
     9301 cctgcaatgc ttcgacaccg gcggaagaga ccagagattc aaactcctgg aggtcttcca
     9361 tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca gcatcataac
     9421 ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg aacgcaggat cgctggctcc
     9481 ccgtgtaaaa aaaacagccc gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa
     9541 gtattctgcg cgctgctacc atgatggtag ttactgctgg taccgccacc ggcgttgtta
     9601 ctgtgatgag aaaccgggcg agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc
     9661 tggctgaccg tgtttttcaa caggatcacg aactgatcaa aagactcgat ttgcccttgc
     9721 agcttaatac cattcaccaa ataaatagaa actggaacac gttcccgacg cagtgcgttc
     9781 aggaacggat cttgtaaaga ttgcccctta gccattctct cttttcctta tatgcttatt
     9841 tgtactttga acctttcgat tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat
     9901 tcagcctgcg atagcaccaa caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc
     9961 actatcaagc cagtgaaccc cttcccaacc acgcagccag gttatctgcc gcttcgccaa
    10021 ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg tatgagattt cgccttcaag
    10081 gtaagaccac atctggcgat aacccacgca acgaatggaa ggcaagtccg tatgcaaatc
    10141 tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa
    10201 acgctgctca atgcgttgat ggagcagttc acggctcgcc ggggcgatgg cgaactgatg
    10261 cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga
    10321 aatgaaaaaa acttccagtg cccgggaaag cctttgtgga tcatttggat gaatccttgc
    10381 tgccgcaacc ggatcgatct cctgaagttg acgatgcaat gactcccaac cttgctctgc
    10441 cgcctgttgc tcaattctgg cccgtacttc cgggtctgcc gacggtagcg gcgacaaccc
    10501 ttccagcaat gccttgaaat acaacatcgt accgcccact aacagtggga tccgccccgc
    10561 cgcggtgata tcggccattt ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc
    10621 ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt
    10681 cggcttcgcc gtcccgatat ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa
    10741 ctctactggt aaaattttac gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg
    10801 ccccatcaaa aaaatcgcct taggcaggct cgccttactg atatcactca tctttcaggg
    10861 cttttatcgc cggatgtaaa tcaacagatt gtaacagacc acccggcggc gttttcacaa
    10921 gttgcggaca taaccgttcc acgtccgcca gcagggttat ggcctgtgcc attgaccact
    10981 gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc aatattgcca ggttcgaata
    11041 cggactgctt cgccaggtag cctatcagtt caggaatcaa gatttgtaaa ttttgttggc
    11101 gtaagggtaa aggcacggcc ctgatggtca catgctgtgc atctgactgg aaatcaatac
    11161 ccaattccgc cagggcagac tgcgcttttt ctaatgccga tttttcttcg ccagaaactt
    11221 ttagccgcaa cggaatcagc aacggctggg cgcaaacggg cgcttcaccc ggcgtcaatt
    11281 gtgcctgacg cagccaacgt tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc
    11341 gctccagcaa cgcacagtcg gaatggacga tagtcagtac ccgaccaaaa ctctgactgt
    11401 tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg tttttgcatc ggcgcgggcg
    11461 tttgcaaaag ctggcgatac acttcacctt gctgtttctg gtaacctggc tgcgcattcg
    11521 gccagggggc agccggacga ctgcctgagg cgggcgcagg agtgtaacgc ggagctaccg
    11581 gctcacgagc tgccggttca gcaaaatgat tgcgccccgc cgccacgcgg ttttccggaa
    11641 tagcacgcgg tgcaggttgg ggttcatcgt ccagcgatag cggcgtttcc agctgctgtt
    11701 gtagcacgct cagcacgccc tgatagataa agtcatgcac cagacgcgac tgatggaaac
    11761 gcacttcgtg tttggcgggg tgtacgttga cgtccacctg atgcgggtcg atctccagat
    11821 acagcacaaa tgccggttgt tgatcggccc ccagtttgtc ttcacaggcc tggcggatcg
    11881 catgattgat caggcgatcg cgcatcatgc gaccattcac gtagcaatac tgaatttctg
    11941 ccagggcggg cgtggtgtga ttcggatcgg ccacccagcc gcgcagggtg agatcgccgt
    12001 gttgccattc aatcgccagc gcttgttcga gaaaagcggt gccgcaaatc gcgcctaagc
    12061 gccgttcttt ttgcccgcct tccggcactg cacggtactg acgcacaatt ttaccgttat
    12121 gcgacaggtt gatcgtgacg tcgaaacgcg ccagcgcaat gcggcggatg atttcatcga
    12181 tatggttaaa ttcagttttc tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga
    12241 acagatccag cacctccagc gtcgttccca ccggatgcgc cgccggttta accgtcacgt
    12301 ccatatcgcg cccttcggca taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag
    12361 tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc ctcaccgcga aagccaaggc
    12421 tgataatggc ttcgagatcg tccagagagg cgattttact ggtggcatga cgagccagcg
    12481 ccagcgccag ctcatctttt ttgataccgc agccgttatc acgaatgcgg ataagtttcg
    12541 ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca
    12601 ccagttcttt gactaccgac gcaggtcgct cgaccacctc acctgcggca atctggttcg
    12661 ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat
    12721 ctggcgtcgt caccgtactg gcagtttgtg ccgttgcacc ctgcggcgca gattgcatcg
    12781 gatgcgcaag gaaataattg cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt
    12841 aatcatcgct cgccagcaaa cgttcttcgc tgttgttgct gataaaaccg gtttcgacca
    12901 gtactgatgg gatatccggc gaacgcagaa cgccaaggct ggcatgttct ggccgacgtt
    12961 tatgtatttc gccaatgcgt tgcaactgac tgatcatact ggtcgctaca tcatacccta
    13021 cccgctggga atgaccgaac tgtaaatcca gcaccgcctg gcttaaatag gggtcagact
    13081 gactgttcgc cagcacatca cccgccccgc ccagcagctc cgactgtttc tcgtgctgct
    13141 ccagccagct ggccatttcg ctgttggcgc gacggttaga gagcacccat acggaagcgc
    13201 cagtcgcact gcggttcggt gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt
    13261 gcttacgtgc cacatcgctg cgccccatca ccgagataaa gtaatccccg tcacgggtta
    13321 aaacgccttt aaacatcgga tcgtcattga gcaaagtacg caatttacgc gcgatggcga
    13381 tggtgacatt tttctcccgc gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt
    13441 gtccggcatc aatagcgatg ataattttat cgccagtgtt agccgtcgcg cgcgctgccg
    13501 gacgcgttac cgtattactg ctgataacac ccgtagtgcg gttactttcc gttttaaacg
    13561 gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgt aggcgtttca acgcgtttcg
    13621 caaccacagg cggcggagga ggcggtggcg catcggcgtt aatcgtaaag acgacagtgt
    13681 aattgctgcc attctgccgc ttcaccgctt cggttttacc attttcggta agatcgacca
    13741 ccagccgtag cgtttgtgca tctttaggcg ttccggagcg aatcgccttc accagattat
    13801 taccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata tcgagcgcca
    13861 cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa ctcaacgtta
    13921 tccgcgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg gcacccacct
    13981 gcgcgcacag cagcagcaac gtcgctacca accaatttct gatgcgatac atcatcccgc
    14041 cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca gaggaaaccg
    14101 cacttacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg acatccgggt
    14161 caggaagaac acctgtacct tgttgtggcc actccaccag gcagatggca tcgttggcaa
    14221 aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg tacaaatcaa
    14281 agtgataaac cattaagttg tcgagcgtat agggttcgac cagcgtataa gtggggcttt
    14341 tgacattacc ctgatgaccc aaagcctgca aaaagccccg gctaaaggtg gttttacctg
    14401 cgcctaaatc gccatacaga tagattacgg ttgcgccatc gcaggctttc gctacccgct
    14461 cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc atcatggttt
    14521 ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc ggttgccagc
    14581 atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg cgcgacacag
    14641 cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc aataatacca
    14701 gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc aataatgcct
    14761 aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc tacgccgcca
    14821 taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc tgctacggaa
    14881 cagcctaaca accgtgcggc ctcgccagga tgcggcgtga tcacgcgatt gtgacgctta
    14941 tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg tttgcgaaaa
    15001 ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc gggaccaatc
    15061 accaccacat cggcccattc caggctttcg ttaagagagt ccatcgtcag ttcatgcacc
    15121 atcaattccg gtcgtgcagt cagcaacggc gcaatgtttt cactgcgggt cagaactcgg
    15181 actaaaccag caccagcacg cagcgccgct tcccccgtca tacgaatagc ccccgccgtg
    15241 ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga agtcgggcga
    15301 cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt cgtctcctga
    15361 cctgccagcc aactatccaa ccccagtgag tcaaaatgca gttgtccggt aacatcccgc
    15421 gcttttccag tgagcaagcc tggtttcagc gcaataaaag tgatggtgtg atcggcgttg
    15481 atcaccgcac ctggcgtagc gccggtttca gccagcaggc cggaagggat atcaagcgcc
    15541 acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga ttcgcggggc
    15601 gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga ttcgggccag
    15661 acaatattcg aagcatggat ctcgccaccc gcgtttaacc aagcttcgcg tgccagcgcg
    15721 gcctcttccg gcaacggttt gtcgctatcc tgggccaaca acgtgacctc aatgccgacc
    15781 gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg accgcacagc
    15841 accagccagt ggcgggcgtc aggataagcc gcgcgacaca cctggaatgc ggcttcgcca
    15901 gcgcgaagca tcagctcata gagtgtgagc cccagcgcat ctgccgcctc gcgttctccg
    15961 cggcggatat cgtcggcgtg ccagacggtg tgtggtatac ttacggggtt tttcttcatt
    16021 gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag
    16081 tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct cagcgagtcc
    16141 gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga ctggatggcg
    16201 cgtcacggta tgctgcgcgc tcgcccccat gagttattgc ccggtacgct gcgcgtgatc
    16261 agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct gaaaaacccc
    16321 aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact tctgcgcaac
    16381 cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa ttttagaccg
    16441 tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg
    16501 acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt tttaggcgaa
    16561 ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg cggcaaatgc
    16621 gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac cgtcgatgct
    16681 cgccgctgta tctcttatct caccatcgaa ctggaagggg cgatcccgga agagttgcga
    16741 ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg cccgtggaat
    16801 cgctattcac aactcactac agaagacgat ttcagcccgc gtaagccgct acatgcgcca
    16861 gaactcattg agttattcgc ctggagcgaa gagaaattct taaaagtcac ggaaggatcg
    16921 gcgattcgcc gtataggtca tctgcgttgg ctgcgtaata tcgccgtggc attaggcaat
    16981 gcgccctggg atgaaacgat tctggcggcg ctggagagtc gcaaaggtga gcacccactt
    17041 cttgatgagc acatagcgtg ggcgatggcg cagcaaatcg agaggcgaaa tgcgtgcata
    17101 gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa agggttaccg
    17161 cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg caattcaaga
    17221 cgaataaaat cgtcaagtga tcgacccgac aaaacggaat cttaattttt aacttatcta
    17281 tcaataggtt aaaaaaatac tattcaccgt gcgaagtttc tttgaaatta agattacagc
    17341 ctgaggctgt ggataactct gtttacaaga gtttgtcaga gacaacaaaa accagcaata
    17401 acgcatcgcg tcgctgtgga tattttattg agagaagaat ttggagcgg
//
LOCUS       DAZUGK010000092        17275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZUGK010000092 DAZUGK010000000
VERSION     DAZUGK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.092"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1375)
                     /gene="djlB"
                     /locus_tag="ACHY5W_002648"
     CDS             complement(<1..1375)
                     /gene="djlB"
                     /locus_tag="ACHY5W_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFS4447526.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLGYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIF"
     gene            complement(1372..2079)
                     /gene="ybeR"
                     /locus_tag="ACHY5W_002649"
     CDS             complement(1372..2079)
                     /gene="ybeR"
                     /locus_tag="ACHY5W_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4447527.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLVASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2244..3221
                     /gene="ybeQ"
                     /locus_tag="ACHY5W_002650"
     CDS             2244..3221
                     /gene="ybeQ"
                     /locus_tag="ACHY5W_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFS4447528.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLELAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3291..3773)
                     /gene="ybeL"
                     /locus_tag="ACHY5W_002651"
     CDS             complement(3291..3773)
                     /gene="ybeL"
                     /locus_tag="ACHY5W_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFS4447529.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4009..6591
                     /gene="leuS"
                     /locus_tag="ACHY5W_002652"
     CDS             4009..6591
                     /gene="leuS"
                     /locus_tag="ACHY5W_002652"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4447530.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6606..7187
                     /gene="lptE"
                     /locus_tag="ACHY5W_002653"
     CDS             6606..7187
                     /gene="lptE"
                     /locus_tag="ACHY5W_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFS4447531.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7187..8218
                     /gene="holA"
                     /locus_tag="ACHY5W_002654"
     CDS             7187..8218
                     /gene="holA"
                     /locus_tag="ACHY5W_002654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4447532.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANCGVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8220..8861
                     /gene="nadD"
                     /locus_tag="ACHY5W_002655"
     CDS             8220..8861
                     /gene="nadD"
                     /locus_tag="ACHY5W_002655"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4447533.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8885..9496
                     /gene="cobC"
                     /locus_tag="ACHY5W_002656"
     CDS             8885..9496
                     /gene="cobC"
                     /locus_tag="ACHY5W_002656"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFS4447534.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9756..10073
                     /gene="rsfS"
                     /locus_tag="ACHY5W_002657"
     CDS             9756..10073
                     /gene="rsfS"
                     /locus_tag="ACHY5W_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4447535.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10077..10544
                     /gene="rlmH"
                     /locus_tag="ACHY5W_002658"
     CDS             10077..10544
                     /gene="rlmH"
                     /locus_tag="ACHY5W_002658"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4447536.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10575..12476
                     /gene="mrdA"
                     /locus_tag="ACHY5W_002659"
     CDS             10575..12476
                     /gene="mrdA"
                     /locus_tag="ACHY5W_002659"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFS4447537.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12479..13591
                     /gene="mrdB"
                     /locus_tag="ACHY5W_002660"
                     /gene_synonym="rodA"
     CDS             12479..13591
                     /gene="mrdB"
                     /locus_tag="ACHY5W_002660"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFS4447538.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13602..14690
                     /gene="rlpA"
                     /locus_tag="ACHY5W_002661"
     CDS             13602..14690
                     /gene="rlpA"
                     /locus_tag="ACHY5W_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFS4447539.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14830..16041
                     /gene="dacA"
                     /locus_tag="ACHY5W_002662"
     CDS             14830..16041
                     /gene="dacA"
                     /locus_tag="ACHY5W_002662"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFS4447540.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16151..16414
                     /gene="ybeD"
                     /locus_tag="ACHY5W_002663"
     CDS             16151..16414
                     /gene="ybeD"
                     /locus_tag="ACHY5W_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFS4447541.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16515..17156
                     /gene="lipB"
                     /locus_tag="ACHY5W_002664"
     CDS             16515..17156
                     /gene="lipB"
                     /locus_tag="ACHY5W_002664"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFS4447542.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHDFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4331 a         4361 c         4421 g         4162 t
ORIGIN      
        1 agaaaataat tttaatgatg cccagacagc ctggcccacc atcgtcaacg ggcattggct
       61 gcggttgcgg ctccggttca acagtttgct tctcggtaaa attcgccgcc tgaattaata
      121 atcgacgaat aattaatgct tccagtggac aacgattttc tgcatcaaga cggcttacga
      181 tcggcattaa tgattcttct gttggttggc tggcctgcac taattgtttg gcaactaaat
      241 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
      301 tgtgcaaaac catctcgacc agtgaacgcc ccagagcaga gagtttttca tcgctgagta
      361 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gatataaagt agcggttttc
      421 cctgatcgtt aaccagttca cgagcttcaa tcatcatcac cactaacggg taataatcga
      481 cccgatgctg acggcaccat tgcaatatta aagcatccgc ctgggtggag ggatactcgc
      541 gccagtagtc acaaagttct gccagcaagc tttcgccttc tccacaatag acgcgttgtg
      601 cgtattcata ataacgcgca tcttcattat cagattcagc ttcccgccac gaccgggcgt
      661 aacccaggag ttcgggaatt cccaggcgac ctgcgctata ccagcggagt aaatttctgt
      721 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
      781 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
      841 gcacggcaac gggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
      901 catataaaaa ggattccagt tcttcttcgt ctatttcccc ggtattttcc tcctgtaacc
      961 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
     1021 ctattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
     1081 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
     1141 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
     1201 atttagcagg cgactgcgca atccggagcg cctcctcata cgcctgacga agttgtttaa
     1261 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
     1321 taatatcgac gtcagtcgtt tcctctattt cgaggatctt ccagcaattt ttcataggtc
     1381 gctcatatcc ttaagcgttt ccgggcatgc agaatagtaa gccaatggtt gccaggacaa
     1441 ggtagggtaa tacgcatcat cactggcaac taattgataa aacatatcat tgccggaatc
     1501 gtcgtattct ttacgcgcta agaattcacg taataactga agattatcat cttcgggaga
     1561 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
     1621 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
     1681 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
     1741 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
     1801 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accaacggtg
     1861 ataaaaccct gccaaccgcg cggcatgacc gttatcgatc attcggtgaa ttgtgcgcag
     1921 caggtcatca cggtcatcaa ttccccagga ggaagaaagg tcaaaaaagc gtgtacctaa
     1981 atacattttg ggtgatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
     2041 ggaaagtgca aacaatattt tttgcgactc catgtccata taatttatcc ttaaatattc
     2101 attgaatgaa tattggccgc attgcgacgc tcgtttattt atacttttct gacgcgacat
     2161 aatacgcata aaataaggct tgcgcaaaaa atgaaacaca taaaaattca atatatttta
     2221 tctacaatca taaggaaatg ataatgattt tcacgtcaag ttgctgcgat aatttatcaa
     2281 tagatgagat tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat attgttgggt
     2341 tttattataa tcgcgatagc gcaattgact ctccggacga cgaaaaggcc ttttactggc
     2401 tggagctggc cgctgagcaa ggtcattgtg aagcacagta ttccttaggg caaaagtata
     2461 ccgaggataa aagccgtcat aaagataatg agcaagccat cttctggctg aaaaaagctg
     2521 ccctacaagg ccatactttc gcttccaacg cccttggctg gatactggat cgtggagaag
     2581 accccaacta taaagaagcg gttgcctggt atcagatagc cgcggagagc ggaatgtctt
     2641 atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa cggagtcgca caagactata
     2701 cgctggcatt tttctggtac aaacaagctg cattacaagg ccatagtgac gcgcaaaaca
     2761 atctggccga tctttatgaa gacggaaaag gcgttgctca aaacgagaca ctcgccgcat
     2821 tctggtattt gaaaagcgca cagcagggta atcggcatgc tcagtttcaa attgcatggg
     2881 attataacgc tggcgaaggg gtggaccagg actataagca agcgatgtac tggtatctga
     2941 aggctgccgc tcaggggagc gtcggcgctt acgtcaacat cggttatatg tataaacacg
     3001 gacaaggcgt tgagaaagat tatcaggctg cctttgaatg gtttacgaaa gccgctgaat
     3061 gcaatgacgc cactgcctgg tataacctgg ccattatgta tcactacgga gaaggaagac
     3121 ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag
     3181 atgtcagtca ggaaattcgt gagactgaag atttactgta gagataaaaa caagggccgg
     3241 ggaatttcat tcggcccata ttctctttat tgctgtgcga aatcagacta ttacggctca
     3301 aacgggcgtc tctggaactg gtcatgacca catttcgggc atagcgtcag cacttccggt
     3361 gtgtagatcg ggagatggaa gtgacatttc tcgcagacca gatttcccag cccgaccact
     3421 tctccgctgt gataaacccc atgatgattg aggtcctgga aaacttcgcg ccattcaagc
     3481 tgcgttttat cggtgatgtc tgccagctcc tgccacaagc tttctttaat cacccgcata
     3541 aagacgctgt cagattcttc tttcaggctc tcttcatagc tcatggcgaa ctcttccagg
     3601 tcacgtctga cagctcgcgt cagctcatcg acctcggttc gcgttaactc ccctgttttt
     3661 attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg
     3721 cgttcgctca gtgacgcaac cagttcacgg taatattgag caaccttgtt catcgtttca
     3781 tctcccgggt cgttaactga ctataataat agacgttctt tgttccttgc tttgctaata
     3841 cgcccacaaa ctctgtaaat taatgtatga ataatccggg catcatttca acgtcgagtg
     3901 cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta tgcggatctg aaaaaccacc
     3961 tctaacgcta cttttgtagc cgtaatgaaa acaggaccac tggctgccat gcaagagcaa
     4021 taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt
     4081 gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct
     4141 tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc
     4201 taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc ctttggtctg
     4261 cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac
     4321 atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag
     4381 ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat
     4441 aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc
     4501 gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa
     4561 cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac
     4621 gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc
     4681 ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc
     4741 gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt
     4801 catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa
     4861 tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat
     4921 actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac
     4981 ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc
     5041 gactacgagt ttgcctctaa atacggcctg aacatcaaac cggttatcct ggcagctgac
     5101 ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct
     5161 ggcgagttca acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact
     5221 gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttcccgt
     5281 cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg
     5341 accccggacg accagctgcc ggtgatcctg ccggaagatg tggtaatgga tggcattacc
     5401 agcccgatta aagcagatcc ggagtgggcg aaaactaccg ttaacggtat gccagcactg
     5461 cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact
     5521 tgcccgcagt acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg
     5581 gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc
     5641 cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg
     5701 tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac
     5761 tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg
     5821 aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag
     5881 aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg
     5941 tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggcgtggaa
     6001 ggggctaacc gcttcctgaa acgtgtctgg aaactggtgt acgagcatac tgcgaaaggt
     6061 gatgttgcgg cactgaacgt tgatgcactg actgaagatc agaaagcgct gcgtcgcgat
     6121 gtgcataaaa ctatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc
     6181 gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccgac cgatggcgag
     6241 caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc
     6301 accccgcaca tctgcttcac gctgtggcag gaactcaaag gcgaaggcga tatcgacaac
     6361 gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg
     6421 caggttaacg gtaaagtccg tgctaaaatc accgttccgg tggacgcaac ggaagaacag
     6481 gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttactgta
     6541 cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag
     6601 gaagcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt
     6661 gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg
     6721 actcaggtga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg
     6781 gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta
     6841 aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc
     6901 agatgatcat gacggtcaat gcgaccgtgt tgatccccgg ccgtgacatc tacccgatta
     6961 gcgccaaggt cttccgttcg ttctttgata acccgcaaat ggcgttagcg aaagataacg
     7021 aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc
     7081 tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg actacaacgg
     7141 atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta
     7201 cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg
     7261 taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca
     7321 aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt
     7381 ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc
     7441 agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca
     7501 tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc
     7561 ctggtttact gctcttgcga attgcggcgt gcaggtgacc tgtcagacac cggagcaggc
     7621 tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc
     7681 tgcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact
     7741 ggagcgttta tcgctgctct ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc
     7801 ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa
     7861 aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg agccggttat
     7921 tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc
     7981 ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat
     8041 gatgggcgag gcgttaaatc gcttaagtca gccgcagtta cgtcaggccg tgcaactcct
     8101 gacacgaact gaactcaccc tcaaacaaga ttatggtcag tcagtgtggg cagagctgga
     8161 agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata
     8221 tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctaa
     8281 aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaata atccctaata
     8341 atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg
     8401 aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg
     8461 ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaagga ccggacgtgc
     8521 cgctggcgtt tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg
     8581 aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa
     8641 tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc
     8701 ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg
     8761 cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac
     8821 cggtattgac ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg
     8881 catgatgcga ctgtggttaa ttcgtcatgg cgaaacacaa gcgaatgtcg atggtctgta
     8941 cagcggtcat gcgcccacac ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca
     9001 tacactgcta gatgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca
     9061 gcataccgcg cgactggttc tcagtgaccg ccagctcccc gttcatatca tccctgaact
     9121 caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga
     9181 tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga
     9241 aggatttcag gcattttcgc aacgtgtgga acgctttatc gcaaggctta gtgaatatca
     9301 gcactatcag aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg
     9361 tttaattggc atgcctgccg agtctatgtg gcattttcgc gttgaccagg ggtgttggag
     9421 cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg
     9481 ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc
     9541 cccggacagg ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa
     9601 attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc
     9661 gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac
     9721 catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt
     9781 ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg
     9841 gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta
     9901 tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg
     9961 tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg
    10021 tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga
    10081 agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg
    10141 agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa
    10201 aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg
    10261 cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc
    10321 cgcagttagc cgctgagctg gaacgctgga agctggatgg gcgcgacgtc agtctactga
    10381 ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggctgagcag agctggtcgc
    10441 tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc
    10501 gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataagag agctttgagt
    10561 agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc
    10621 tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta
    10681 tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg
    10741 aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaacg
    10801 gtatccctct ggccctcaac cgcactatct accagataga aatgatgccg gagaaagtcg
    10861 ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt agatctgacc gatgacgata
    10921 ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa
    10981 ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg
    11041 ttgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca
    11101 tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac
    11161 tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag
    11221 atgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta
    11281 tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc
    11341 tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca
    11401 ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaacc
    11461 tgtttgttga cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac
    11521 cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg
    11581 tggcggtttc ggcattaagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag
    11641 gctggtggca actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg
    11701 gacgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg
    11761 cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt
    11821 acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac
    11881 agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg
    11941 gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg
    12001 gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat
    12061 gggtacaacc gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga
    12121 aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca
    12181 gcgcaccgta caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga
    12241 acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg
    12301 cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggaa aacggcggcg
    12361 cgggtccggc ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata
    12421 acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat
    12481 gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct
    12541 gctgatttta ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca
    12601 ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt
    12661 ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat
    12721 ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg
    12781 ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc
    12841 actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaacactgg
    12901 catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg
    12961 aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg
    13021 gcgtctgatt ggcgtcgcag tagtgctggt agcggcgttc attccgattc tgtggttctt
    13081 cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc
    13141 actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg
    13201 cggcaaaggc tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac
    13261 tgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct
    13321 cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac
    13381 ctttggtcgc gtcatggctg gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa
    13441 tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta
    13501 tggaggatcg gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac
    13561 ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg
    13621 ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga
    13681 cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg
    13741 acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa
    13801 gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg
    13861 atgccgaacc aggcagtaac ctgacggcct ctggcgaagc attcgatccg acacagctga
    13921 cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg
    13981 ggcgaatgat cgtggtgcgc attaatgatc gcggtcctta cggcaacgac cgcgttattt
    14041 cgctttctcg cgcagcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg
    14101 atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag
    14161 tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt aagcggcggc gcaggaacaa
    14221 gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagtg
    14281 aagatccgac cggcgcgccg gtaaccagca gcggtttcct cggcgcacca acgaccctag
    14341 cgccaggtgt actggaaggc agcgaaccga cgcctgctcc acagcccgtt gttacagctc
    14401 cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg
    14461 ccagcggcaa ctttatggtg caggtcgggg ccgttagcga tcaggctcgt gcgcaacagt
    14521 accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg
    14581 tctggcggat ccagcttggc ccattcgcca gcaaagccga agccagtacc ttgcagcaac
    14641 gtttgcaaac cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag
    14701 gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc
    14761 ctgccggtag tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta
    14821 gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg
    14881 gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg
    14941 atcccgggtg taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc
    15001 aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg
    15061 atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta
    15121 gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg
    15181 atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg
    15241 caatcgggta acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct
    15301 tttgttggct tgatgaacag ctacgttaac gccctgggcc tgaaaaatac ccacttccag
    15361 acggtacatg gtctggatgc tgatggtcag tacagctccg cgcgcgatat ggcgctgatc
    15421 ggccaggcat tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt
    15481 acgtttaacg gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat
    15541 gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg
    15601 actgaaggcc agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt
    15661 gaagccgaaa gtaaaaaact gttaacctgg ggcttccgtt tcttcgaaac cgttaaccca
    15721 ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct
    15781 tcgttagggg ttgataaaga cgtgtacctg accattccgc gtggccgcat gaaagatctg
    15841 aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc
    15901 gtcggaacta tcaacttcca gcttgatggc aaaacgatcg aacaacgccc gctggttgtg
    15961 ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg
    16021 ttccatcact ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata
    16081 ctaagcatca gtaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc
    16141 cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta
    16201 caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg
    16261 ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc
    16321 ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact
    16381 gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac ccggcccctg
    16441 gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc
    16501 acggagatgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt
    16561 acgagccaat ctcccaggct atgcatgact tcaccgatac ccgcgatgat agtacccttg
    16621 atgaaatctg gctggtcgag cactatccgg tattcaccca agggcaggca ggaaaggcgg
    16681 agcacatttt aatgcccggt gatattccgg tgattcagag cgatcgcggt gggcaggtga
    16741 cttatcacgg gccggggcaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac
    16801 tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac
    16861 tgggtataga agcgcatcct cgtgctgacg cgcctggtgt ctatgtaggg gaaaagaaaa
    16921 tttgctctct gggtttacga attcgacgcg gttgttcatt ccacggtctg gcattaaacg
    16981 tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgcc ggaatggaaa
    17041 tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgct ccacgtttac
    17101 tggaaaatat tttagcgcta ctaaacaatc ctgacttcga atatattacc gcttaattcc
    17161 atacatcaat ggcccaattt acactgggtc attactcata atacctgcta atgctttaca
    17221 tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcgatgct acgcc
//
LOCUS       DAZUGK010000093        17142 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZUGK010000093 DAZUGK010000000
VERSION     DAZUGK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.093"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..1368
                     /gene="hpaG"
                     /locus_tag="ACHY5W_002665"
     CDS             79..1368
                     /gene="hpaG"
                     /locus_tag="ACHY5W_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFS4447543.1"
                     /translation="MKGTIFAVALNHRSQLYAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFLPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHY5W_002666"
     CDS             1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHY5W_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4447544.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHY5W_002667"
     CDS             2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHY5W_002667"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFS4447545.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEAIKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3694..4074
                     /locus_tag="ACHY5W_002668"
     CDS             3694..4074
                     /locus_tag="ACHY5W_002668"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFS4447546.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4142..4945
                     /gene="hpaH"
                     /locus_tag="ACHY5W_002669"
     CDS             4142..4945
                     /gene="hpaH"
                     /locus_tag="ACHY5W_002669"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFS4447547.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4956..5744
                     /gene="hpaI"
                     /locus_tag="ACHY5W_002670"
     CDS             4956..5744
                     /gene="hpaI"
                     /locus_tag="ACHY5W_002670"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFS4447548.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPGVY"
     gene            5919..7295
                     /gene="hpaX"
                     /locus_tag="ACHY5W_002671"
     CDS             5919..7295
                     /gene="hpaX"
                     /locus_tag="ACHY5W_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFS4447549.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKANWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPHICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7305..8195
                     /gene="hpaA"
                     /locus_tag="ACHY5W_002672"
     CDS             7305..8195
                     /gene="hpaA"
                     /locus_tag="ACHY5W_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFS4447550.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8446..10008
                     /gene="hpaB"
                     /locus_tag="ACHY5W_002673"
     CDS             8446..10008
                     /gene="hpaB"
                     /locus_tag="ACHY5W_002673"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFS4447551.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWSTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQINQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10026..10538
                     /locus_tag="ACHY5W_002674"
     CDS             10026..10538
                     /locus_tag="ACHY5W_002674"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFS4447552.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10931..13081
                     /gene="btsT"
                     /locus_tag="ACHY5W_002675"
     CDS             10931..13081
                     /gene="btsT"
                     /locus_tag="ACHY5W_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFS4447553.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDQETPYVPIPEGGVKISSHH"
     gene            13131..13334
                     /locus_tag="ACHY5W_002676"
     CDS             13131..13334
                     /locus_tag="ACHY5W_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS4447554.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13345..14301
                     /gene="yjiA"
                     /locus_tag="ACHY5W_002677"
     CDS             13345..14301
                     /gene="yjiA"
                     /locus_tag="ACHY5W_002677"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS4447555.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAELKK"
     gene            complement(14379..16919)
                     /locus_tag="ACHY5W_002678"
     CDS             complement(14379..16919)
                     /locus_tag="ACHY5W_002678"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFS4447556.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESSYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETIMVKNGQAETFTVWRTVHGNIIQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVDTLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAVVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKSQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEEARHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
BASE COUNT         4021 a         4666 c         4530 g         3925 t
ORIGIN      
        1 ttgccaaact acgcaatcgg gaataaaagt aatcattaac atattaatga ttaagatgat
       61 cgaatccgag gagtggtaat gaaaggcact atcttcgccg tagcattgaa ccatcgcagc
      121 caactttatg cctggcagga agcgttccag caatccccct acaaagcccc gcctaaaact
      181 gcggtctggt ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt
      241 ccacagggtg aaaaggtact gagcggtgcg acagttgcgc tgattgtggg aaaaacggcg
      301 acgaaagtac gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac
      361 gtcagcctgc cggaagagag cttttaccgt ccggcaatca aagcaaaatg tcgtgatgga
      421 ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg ataatctgac tatctatacc
      481 gagatcaacg ggcgtcctgc cgatcactgg aacaccgccg atttacaacg taacgccgca
      541 cagctgctga gcgccctgag cgaatttgcc acgctgaacc caggcgatgc cattctgctc
      601 ggcacgccac aggcgcgcgt ggaaatacag ccaggcgatc gcgtgcgtgt tctcgcagaa
      661 ggtttcctgc cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcgcaagagc
      721 ttcccaacgc tgccacaccc gcacggtacg ctgtttgccc tcggcctgaa ctacgccgac
      781 cacgccagcg aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca
      841 aataccctca ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac
      901 tatgaagcgg agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat
      961 gccatggatt atgtcgcggg ctacaccgtg tgtaacgact acgccattcg cgactatctg
     1021 gaaaactact accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt
     1081 tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc
     1141 gtcaacggcg agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc
     1201 ctgatcgcct acttaagcga atttatgacc ctgaatccgg gcgacatgat cgccaccggc
     1261 acaccaaaag gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc
     1321 gtgggccgcc tggtgaaccg aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc
     1381 attggatcaa cggcaaaaat gtcgcaggta acgactactt ccagaccacc aatccggcaa
     1441 cgggtgaagt gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag
     1501 cggcagcgaa agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc
     1561 tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc tgagatcgcc gcgatggaaa
     1621 ccgcggacac gggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttctc
     1681 acaactttga atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg
     1741 acgacaagat gctcaactac acgctggtgc aaccggtagg cgtttgtgcg ctggtatcgc
     1801 cgtggaacgt gccgtttatg accgccacat ggaaggtcgc gccgtgtctg gctctgggca
     1861 ataccgcggt gctgaagatg tcggaactct ccccgctgac cgctgaccgc ctgggtgagc
     1921 tggcgctgga agccggtatt ccggcgggcg tgctgaacgt ggtacagggc tacggcgcaa
     1981 ccgcagggga tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta
     2041 cggccaccgg gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg
     2101 gcggtaaatc gccggtgctg atttttgaag atgccgatat tgaacgtgcg ctggacgctg
     2161 ccctgttcac catcttctca atcaacggcg aacgctgcac cgccggttcg cgcattttta
     2221 ttcaacagag catctatcca gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc
     2281 gcgtgggcga tccgaacgat ccgaacaccc aggttggggc gcttatcagc cagcagcact
     2341 gggaaaaagt ctccggctat atccgtctag gcattgaaga aggcgcaacc ctgctggcgg
     2401 gcggtccgga taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc
     2461 caacggtgct ggcggacgta gataaccgta tgcgcgttgc ccaggaagag attttcgggc
     2521 cggtcgcctg cctgctgccg tttaaagatg aagccgaagg cttacgcctg gcaaacgacg
     2581 tggagtacgg cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg
     2641 cgcgtggcat tgaagcaggc atggtgttcg tcaacaccca gaacgtgcgc gacctgcgcc
     2701 agccgtttgg cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg
     2761 aagtgttcgc ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat
     2821 ggggagtctg atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg
     2881 tatctctctg aactaccagg aaaaaaccac ggttgtcgcc agggtgcgat cgacggacat
     2941 aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc
     3001 cactggctgg tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac
     3061 accagcaacg agctgccgca ctttattcgc gacatgacct acaactacga aggcaacccg
     3121 gagttggggc agctaattgc cgatgaagca ataaaactcg gcgtgcgggc gaaagcgcac
     3181 aacattccca gcctgaaact ggagtacggc acgctggtac cgatgcgcta catgaatgaa
     3241 gacaagcact tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac
     3301 agccgcaagc tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg
     3361 gtccttgcca gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg
     3421 atgaacagct acacccgcga gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg
     3481 cgcgaaggcc agttcaaaga gttttgcaat atgctgccgg agtacgccga ctactgctac
     3541 ggcgaaggca atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac
     3601 gacggcaagg tggagtttat taccgagctg ttcccaagct ctggcaccgg tcaggttaac
     3661 gctgttttcc cgctgcccgc gtaaggacgt tttatgccgc actttatcgt ggaatgtact
     3721 gaaaatattc gtgaagaagc ccgcttacct gagctgtttg ccagtgttaa cactgcactg
     3781 gcggcaacgg ggatttttcc gcttggggga atacgtagcc gggcgcactg gatcgatacc
     3841 tggcaaatgg ctgatggcaa gcacgattac gcctttgtgc atatgacgct aaaaatcggc
     3901 agcggacgca gtctggaaag ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa
     3961 acgcacttcg cctcactgat ggagagccgt tacctggcgc tttcatttga gattgcagag
     4021 ctgcacccga cgttgaattt caaacagaac aacgtacacg cgttgtttaa ataaatttgc
     4081 accagcttcc aggccggatg ataaggcgct gccgcatccg gcaatgccac aggatatcgc
     4141 tatgttcgat aaacacaccc acaccctgat cgcccagcgt ctggatcagg cagaaaaaca
     4201 gcgcgaacag atccgcgcga tctccctgga ttaccccgag atcaccatcg aagacgccta
     4261 cgccgtgcag cgcgaatggg ttcgcctgaa aatcgccgaa ggccgtacgc tgaaaggcca
     4321 caaaatcggc ctgacctcga aagctatgca ggccagctca caaatcagcg agccggatta
     4381 cggtgccctg ctggatgaca tgttcttcca cgatggcagc gatatcccga ccgatcgctt
     4441 tatcgtgccg cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc tgcgtggacc
     4501 aaactgcacg ctgttcgacg tttacaacgc cacggactat gtgatcccgg cgctggagct
     4561 gatcgacgcc cgctgccaca acatcgatcc ggaaacccag cgcccgcgca aagtgttcga
     4621 caccatttct gataacgccg ccaatgccgg ggtgatcctc ggtggtcgtc cgatcaaacc
     4681 tgacgagctg gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg tgattgaaga
     4741 aaccggcgtc gccgctggtg tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa
     4801 caaactcgca ccgtatgacg tacaactgga agccgggcaa atcattctcg gcggttcgtt
     4861 tacccgcccg gttccggcgc gtaagggcga caccttccac gtcgattacg gcaacatggg
     4921 ctccattagc tgccgctttg tttaaggaga gaacgatgga aaacagtttt aaagcggcgc
     4981 tgaaagcagg ccgcccgcag atcggattat ggctggggct gagtagcagc tacagcgcag
     5041 agttactggc cggagcagga ttcgactggt tattgatcga cggtgagcac gcgccgaata
     5101 acgtgcaaac cgtgctcacc caactacagg cgattgcgcc ctaccccagc cagccggtag
     5161 tacgtccgtc gtggaacgat ccggtgcaaa tcaaacaact gctggatgtc ggcacacaaa
     5221 ccttactggt gccgatggta caaaacgccg acgaagcccg tgaagcggta cgcgccaccc
     5281 gttatccccc cgccggtatt cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga
     5341 atcgcattcc tgattacctg caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg
     5401 aaacccgtga ggcaatgaag aacttaccgc agattctgga cgtggaagga gtcgacggcg
     5461 tgtttatcgg cccagcggat ctgagcgccg atatgggtta cgccggtaat ccgcaacacc
     5521 cggaagtgca ggccgcgatt gagcaggcga tcgtgcagat ccgcgaagcg ggtaaggcgc
     5581 cggggatcct gatcgccaat gagcaactgg caaaacgcta tctggaactg ggcgcgctgt
     5641 ttgtcgccgt cggcgttgac accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac
     5701 ggtttggcgc gcaggctaca gcgattaagc ccggcgtgta ttaatgccgg atgcgctgcg
     5761 cttatccggc ctacactcgc accaaaccgt aggccggata aggcgtttac gccgcatccg
     5821 gcaaaaagcc gtaccaaatc gcggaccgga taaggcgttt gtgcgcatcc gccataaacc
     5881 ttgtcgtacc ctacaaaaat cccattagag gaagaaaaat gagcgacacc tcacctgcca
     5941 taccggagag tatcgatccg gcgaatcagc ataaagcgct gactgccgga caacaggcgg
     6001 ttattaagaa gctatttcgc cgcctgatcg tctttctgtt cgtgctgttt atcttctcgt
     6061 tccttgatcg catcaacatt ggttttgccg gactaactat gggacgcgac ctcggtctga
     6121 gcgccaccat gtttggcctc gctaccaccc tgttctatgc cgcttatgtc atcttcggca
     6181 ttcccagcaa cattatgctg agtattgtcg gtgcgcgacg ctggatcgcc accatcatgg
     6241 tgctttgggg catcgcctct actgccacca tgtttgccac tggccccacc agcttatacg
     6301 tactgcgtat actggttggc attaccgaag ccggctttct gcctggcatt ctgctctatt
     6361 taaccttctg gtttccggcc tatttccgcg cccgtgccaa cgccttgttt atggtggcaa
     6421 tgccggtaac gacagcgttg ggatcgatcg tttccggcta cattttgtcg ctggatggcg
     6481 taatggcatt aaaaggctgg cagtggctgt ttttgctgga aggcttcccg tcggtattac
     6541 tcggcgtcat ggtatggttc tggcttgatg actcaccgga caaagctaac tggctgacga
     6601 aagaagacaa aaaatgcctg caagagatga tggataacga tcgtctgacg ctggttcagc
     6661 cagagggagc catcagccac cacgccatgc aacaacgcag catgtggcgg gagatcttca
     6721 ctccggtggt gatgatgtat accctggcgt atttctgcct gaccaacaca cttagtgcga
     6781 tcagcatctg gacaccgcag atcctgcaaa gctttaatca gggcagcagt aatatcacca
     6841 tcggcctgct ggccgccgta ccgcacattt gtaccattct cgggatgatc tactggagcc
     6901 gtcactcaga tcgccgccag gaacgaaggc atcacaccgc ccttccttat ttgttcgctg
     6961 ccgctggttg gttactggct tcggcaactg atcacaacat gatccagatg ctggggatta
     7021 ttatggcttc gaccggatca ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt
     7081 ccatcagcct gcgggcacga gcgatcggta ttgcggtgat caacgccact ggcaacattg
     7141 gttcagcatt aagtccgttt atgatcggct ggttgaaaga tctgaccggc agctttaaca
     7201 gtggattgtg gtttgttgcc gcgctgctgg tgattggtgc agggattatc tgggcaattc
     7261 caatgcagtc ctcccgtccg cgagcgaccc cgtaaggaac gacgatgtgt gaccgtcaga
     7321 ttgccaatat tgatatcagc aaagagtacg atgaaagcct gggcacggac gatgtgcatt
     7381 atcagtcctt cgcccgcatg gcggcatttt ttggccgcca tatgctgcca catcgccacg
     7441 aacagtactt tcagatgcat ttcctcaata gcggacagat tgagctacag cttgacgatc
     7501 atcgctactc ggtggaagcg cccctgtttg tcctgacgcc gccgtcagta cctcatgcgt
     7561 ttattacgga gtctgacgcc gacggtcatg tgttgacggt acgggaagat ctgatctggc
     7621 ccctgctgga agttctttat ccaggcactc gggaaacctt cggcctgccg gggatttgcc
     7681 tgtcactggc agataaaccc gacgaactgg cggcgctgga acactattgg caactgatag
     7741 aacgggaatc ggtggaacaa ctgcctggac gggaacacac cctgacgctc ctggcacagg
     7801 cagtgttcac cctactgctg cgtaacgcaa aactcgacga ccatgccgcc agcggaatgc
     7861 gcggagaatt aaaactgttc cagcgttttc atatgctgat tgaaagccat tttcatcagc
     7921 actggacagt accggattac gctaacgaac tgcatatcac cgaatcacgc ctcacggaca
     7981 tctgccgccg ctttgccaac cgtccgccaa aacggttgat tttcgacagg cagctgcgag
     8041 aagccaagcg gctgctgctg ttttctgata acgccgtgaa caatattgcc tggcaactcg
     8101 gttttaagga tccggcttat tttgcgcgct tttttaatcg tttagtcggt tgctcgccca
     8161 gtgcttaccg tgccaaaaaa gtacctgtga cgtgaaaatc ccttagcctt aacgggaaac
     8221 caggcatcac ctgctattcc tctttctccg cctggagcaa ggggatttag taacgtcaaa
     8281 aatagctaaa agcgacctcg atcacaaaac cgtacccgac ctgaaaagta ccagccattg
     8341 tcccaaaagt ctcttcccgt aatcgcccct gcaagcttca attgaataac aaaaacaaca
     8401 cacaaaatta acaattgtat tccgattaat actgtagagg tcgatatgaa accagaagat
     8461 ttccgcgcca gtacccaacg tccgttcacc ggggaagagt atctgaaaag cctgcaggat
     8521 ggtcgcgaga tctatatcta tggcgagcga gtgaaagacg tcaccactca tccggcattt
     8581 cgtaatgcgg cagcgtctgt tgcccaactg tacgacgcgc tgcacaaacc ggagatgcag
     8641 gactctctgt gttggagcac cgacaccggc agcggcggct atacccataa attcttccgc
     8701 gtggcgaaaa gtgccgacga cctgcgccag caacgcgatg ccatcgctga gtggtcacgc
     8761 ctgagctatg gctggatggg ccgtacccca gactacaaag ccgctttcgg ttgcgcactg
     8821 ggcgcgaatc cgggctttta cggtcagttc gagcagaacg cccgtaactg gtatacccgt
     8881 attcaggaaa ctggcctcta ctttaaccac gcgattgtta acccaccgat cgatcgtcat
     8941 ttgccgaccg ataaagtgaa agacgtttac atcaagctgg aaaaagagac tgacgccggg
     9001 attatcgtca gcggtgcgaa agtggttgcc accaactcgg cgctgactca ctacaacatg
     9061 attggcttcg gctcggcaca agtgatgggc gaaaacccgg acttcgcact gatgttcgtt
     9121 gcgccaatgg atgccgatgg cgtgaaatta atctcccgcg cctcttatga gatggtcgcg
     9181 ggtgctaccg gctcgccgta cgactacccg ctctccagcc gcttcgatga gaacgatgcg
     9241 atcctggtga tggataacgt actgatcccg tgggaaaacg tgctgatcta ccgcgatttc
     9301 gatcgctgcc gtcgctggac gatggaaggc ggttttgccc gtatgtatcc gctgcaagcc
     9361 tgtgtgcgcc tggcagtgaa actcgacttc atcacggcac tgctgaaaaa atcactcgaa
     9421 tgtaccggca ccctggagtt ccgtggtgtg caggccgatc tcggtgaagt ggtagcgtgg
     9481 cgcaacacct tctgggcatt aagtgactcg atgtgttcag aagcgacgcc gtgggtcaac
     9541 ggggcttatt taccggatca tgccgcactg caaacctatc gcgtactggc accaatggcc
     9601 tacgcgaaga tcaaaaacat tatcgaacgc aacgttacca gtggcctgat ctacctccct
     9661 tccagtgccc gtgacctgaa taacccgcag atcaaccagt atctggcgaa gtatgtgcgc
     9721 ggttcgaacg gtatggatca cgtccagcgc atcaagatcc tcaaactgat gtgggatgct
     9781 attggcagcg aatttggtgg tcgtcacgaa ctgtatgaaa tcaactactc cggtagccag
     9841 gatgagattc gcctgcagtg tctgcgccag gcacaaagct ccggcaatat ggacaagatg
     9901 atggcgatgg ttgatcgctg cctgtcggaa tacgaccaga acggctggac tgtgccgcac
     9961 ctgcacaaca acgacgatat caacatgctg gataagctgc tgaaataacg cagcaggagg
    10021 ttaagatgca attagatgaa caacgcctgc gctttcgtga cgcgatggcc agcctgtcgg
    10081 cagcggtaaa tattatcacc accgagggcg acgccggaca atgcgggatt acggcaacgg
    10141 ccgtctgctc ggtcactgat acaccaccat cgctgatggt gtgcattaac gccaacagtg
    10201 cgatgaaccc ggtttttcag ggcaacggca agttgtgcgt caacgtcctc aaccatgagc
    10261 aggaactgat ggcacgccac ttcgcgggca tgacaggcat ggcgatggaa gagcgtttta
    10321 gcctctcatg ctggcaaaaa ggtccgctgg cgcagccggt gctaaaaggt tcgctggcca
    10381 gtcttgaagg tgagatccgc gatgtgcagg caattggcac acatctggtg tatctggtgg
    10441 agattaaaaa catcatcctc agtgcagaag gtcacggact tatctacttt aaacgccgtt
    10501 tccatccggt gatgctggaa atggaagctg cgatttaagc tccagcccct tcacgtattc
    10561 gtcgaagggg catttttatt gtgatctcaa tcacatatcc acccgatgaa taattcttgc
    10621 ccaaccgctc accgccattc ctgaccgctt acatccctaa aataaccact cagttattta
    10681 ccttacttta cgcgcgcgta tctctggcaa catcactgca ggatagcggt caatttacct
    10741 cctcaaacgc aacgcaaacc tagaacggct tcggcctatg attaacaaat tactctatgg
    10801 attttggttt gcaggaaggc agcaagtgag tgaatcaccg ggagcttaca acagtaagtg
    10861 acaggggtga acgaacgcag ctgccgcacc tgtaagccaa aagacgacga gtaaaatgcc
    10921 aggttttact atggatacga aaaaactatt caagcacata ccctgggtga ttctcggaat
    10981 catcggtgca ttctgcctcg cggtagttgc attacgtcgg ggggagcacg tcagcgccct
    11041 gtggatcgtg gtcgcctctg tgtcggtgta tctggtggcg tatcgctact acagtttgta
    11101 tatcgcccag aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa
    11161 cgacggtctg aactacgttc cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc
    11221 tatcgccggt gctggtccgc tggtcggtcc ggttctcgcc gcacagatgg gctacctgcc
    11281 tggcacgctg tggctgctgg cgggggtcgt gctggccggt gcggttcagg actttatggt
    11341 gctgtttatc tcctctcgcc gtaacggcgc gtctctcggt gagatgatca aagaagagat
    11401 gggaccagta ccggggacta tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct
    11461 cgccgttctg gcgctgattg tggttaaagc cctggccgaa agtccgtggg gtgtcttcac
    11521 cgtttgctca accgtaccga ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc
    11581 ggggcgtgtg ggtgaagtct ctgttattgg tatcgtgctg ctggttgcct ctatctactt
    11641 cggtggcgtg attgctcacg atccgtactg gggtccggca ctgaccttta aagacaccac
    11701 cattaccttc gcgctgattg gctatgcgtt tgtttccgca ttgctgccgg tgtggctgat
    11761 cctcgcaccg cgcgactatc tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc
    11821 gctgggtatc gtggtgctga acccggaact gaaaatgcct gcgatgaccc agtacattga
    11881 cggtactggc ccgctgtgga aaggcgctct gttcccgttc ctgttcatca ccatcgcctg
    11941 tggtgcggta tctggcttcc acgcgctgat ctcttccggt acgacgccga aactgctggc
    12001 taacgaaacc gacgcgcgtt tcattggcta cggcgcaatg ctgatggagt ccttcgtggc
    12061 gattatggcg ctggttgctg cgtccatcat cgaaccaggt ctttacttcg cgatgaacac
    12121 cccgcctgcg ggccttggca tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc
    12181 gccgatcatc atggcgcagc tgaaagacgt taccgcacac gcggcagcga ccgtcagctc
    12241 ctggggcttc gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc
    12301 ttctgtcctg aaccgtgcag gtggtgcgcc gacgctggcg gtaggtatcg ctcacgtgtt
    12361 ccacaaagtg ctgccgatgg ctgacatggg cttctggtat cacttcggta ttctgttcga
    12421 agcactgttc atcctgaccg cgctggatgc gggtacccgt tctggccgct ttatgctgca
    12481 agatctgctg ggtaacttca tcccgttcct gaaaaaaacc gactctctgg ttgccggtat
    12541 catcggtact gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga
    12601 tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt atctccaacc agatgctggc
    12661 agccgtagcg ctggtactgg gtaccgttgt gctgattaag atgaagcgca cccaatacat
    12721 ctgggtaact gttgttccgg ctgtatggct gcttatctgc accacctggg cgctgggcct
    12781 gaaactgttc agcaccaacc cgcagatgga aggcttcttc tacatggcaa gccagtacaa
    12841 agagaagatt gctaacggta ctgacctgac ggcgcagcag attgccaaca tgaaccacat
    12901 cgttgtgaac aactacacca acgcaggcct gagtattctg ttcctgattg tggtgtacag
    12961 catcatcttc tacggtttca aaacgtggct tgcggtgcgt aacagcgaca aacgtactga
    13021 ccaagaaaca ccgtacgttc caatcccgga aggcggcgtg aagatctctt cgcaccacta
    13081 accgtgttta gccccgcttc ggcggggctt tgttcttatc agagtgaact atgtttggta
    13141 acttaggaca ggcaaaaaaa tatctcggcc aggcggcaaa gatgttgatt ggcattccag
    13201 actacgacaa ctacgtcgag catatgaaga ccaaccatcc cgacaagccg tacatgagct
    13261 atgaagaatt cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt aaaggcggta
    13321 tgcgctgttg ttaatggaga gagcatgaac ccgattgcag ttaccctact caccggtttt
    13381 ttaggcgcgg gcaaaaccac cctgctgcgc catattctta acgaacaaca cggctacaag
    13441 attgccgtga ttgaaaacga attcggcgaa gtctccgttg atgatcaatt gattggtgac
    13501 cgcgccacgc agatcaaaac gctgaccaac ggctgcatct gctgttcgcg ctccaacgag
    13561 ctggaagacg cactactcga cctgctggat aatctcgata agggcaatat tcagttcgat
    13621 cgtctggtca ttgaatgcac cggcatggcc gatcccggcc cgattattca gacctttttc
    13681 tcccatgaga ttttatgcca gcgttacctg ctggacggcg tgattgcgct ggtggatgcg
    13741 gtacatgccg atgagcagat gaatcagttc accatcgccc agtcccaggt tggctacgcc
    13801 gaccgtattc tgctgaccaa aaccgacgtc gcaggcgaag cagaaaaact gcgcgaacgc
    13861 ctggcgcgca tcaacgcccg tgcaccggtc tacaccgtca cccacggcga catcgacctg
    13921 ggcctgctgt tcaacaccaa cggttttatg ctggaagaga acgtcgtcag caccaaaccg
    13981 cgtttccact ttatcgcgga taaacaaaac gatatttcgt cgattgtggt ggaactggat
    14041 tacccggtag atatcagcga agtttcccgg gtgatggaaa acctgctgct ggaatcggca
    14101 gataaactgc tgcgttacaa agggatgctg tggattgacg gcgaacctaa ccgcctgctg
    14161 ttccagggcg tccagcgcct ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa
    14221 ccgcatagca cgatggtgtt tatcgggatt cagttgccgg aggaagagat tcgggcggcg
    14281 tttgcggaac tcaaaaagta accagccctc caacatcaca atacctgaga ctttatcacc
    14341 ctcatattcc tcctgaatat gagggctttc gggcttaatt atctctgaac gtgcaacact
    14401 tcctgcgact ccttatgcgc ctccacatcc tgcttcgtta accagagcga cttacgacca
    14461 aaattttcgt acattttcag ctgatcttca tagtgcttat caactgttcc atcgggagca
    14521 ataaatccac tctgaccggg tgcgaccaca tcccaggcaa gcaccggacg atcgcttgtc
    14581 gttggtgaga agacaatcat atcgttttct gttccacggt tttgatactc cgcctgatga
    14641 cgcgcttctt ccgctgcggc ctgcggtacg ccaaagaaat tatttgcccg gaacgttaag
    14701 gccattgcag gtgttttcca gttactcaca ttattgccat agcgtttgga aagagtctcc
    14761 caggtatctt ccagcgcagc caacacaacc tcctgctgtg atttcccggc aaacagatca
    14821 accgcctgtg ggattggtga tttgtctccc tgcaccgcct catacaaaat ttttgctcca
    14881 acacttatat tcagcgaacc agttggaccg tcctgggttg tttcgtagcc actggcgctg
    14941 taccacttat caaatggcat aggtacaaca gccactacgg tacgcttcaa catactggtc
    15001 agccaaacgt tcaggatggc agagcctggc tgctgccagg ttttaccatc atcattaagc
    15061 aaattgatgc catcccaacg tgttaatgta tccaccaact gacggcgcgg atcgctctgt
    15121 gtcaaaccag atgtcgctgc ttgcagagta ggtaaaaaaa gcctcaggtt aagatcctga
    15181 cgactggttt ggcgaataac atcccatgcc tgatcagcag ttaagcgtgg cttttgctca
    15241 agcagtcggt cgatctccgt aacgcgatct gcaccacccc acaaaaaggc aaacagatcc
    15301 gaagcgggat aatctttttg gggagaattg ttccagttag caatatatcc cgactggggg
    15361 ttatacacct tagggttcat ttcaaaaggc aatagccctt tccagtccca ttttcccgtg
    15421 ccaggaacgg gtaatcgcgg atcatggcct gattgacgat ccggataagc accagtatga
    15481 acataaccaa tattgccgtt cacatcagca tagtaccagt tgatggtcag tgcttgtttt
    15541 gctgcctgct gtgtccactc ctgccaattt ttggccttca tctgatgagt ccaggccagc
    15601 aaagacgcca cctctttacc atcccaggcg cgggatttag cgtaagccgt ttgtgtcgtc
    15661 tggtcagttt ggataatgtt gccatgcacc gtgcgccaga cagtaaaggt ctctgcctga
    15721 ccatttttca ccataatggt ttcttcacgg cttaacattt tcacccactt accattatgc
    15781 aagtagtagc ctggtttctc tgccgacagc cgttcagcaa aaatatcgac atcatcgccg
    15841 aaacctgccg ttgatcccca ggaaatcaca ccattatgac caaaaaccag cccaggatag
    15901 gcaaatggtg tattgccagt gacatcataa ccagcaccgt gcagaccaat accataagta
    15961 tacgcaggcg cataccagcc aaactgcgga ccatttacca tgattgcttt cgcatcctgg
    16021 gctttgcttt tgccgatcac ccacatattg ctggtcgttg gataccccgc cagaccattg
    16081 gcaccaccct gtgcaaattg tgcagcaata gtttcccggt tcttccctgc tgttaacgcc
    16141 agcagtgcgc catccgcccc ttttgctggt cggtcaagca ttggtgcagg taaatcgtag
    16201 cgtggcaaca gagctgctgt ttgcgagttt tgctgattaa atttaagtgg gtagctactc
    16261 tcttgtacgg caatagtggt tggcgctgat gggtttacca gccatttcaa ctgattaaat
    16321 accgccatgc cttgtgatac accatattta tcttttaaag ccgttagcag tgccagatta
    16381 tcaatttcgc tagtgctatc agagaagcgg tttgccatgg tgcccacaaa tatcatcgcg
    16441 acatcaaacg gttcccagcg cttaggagta aagccaaatg tattaaactg ttttggtaag
    16501 agcgtctctg gattggtatt taccttatca atccaggcat tcattccatc agcgtagcct
    16561 tgcagaatgg acatatcctc tggggaaagg gcagcaattt gcgcccggat agcatccggc
    16621 cagtagttac gacggatatc tttatcaaat ttcacaaaat ctttgccaag cacttccgcg
    16681 acagtccctt gagtactgcg acgtgccatt tccatctgaa aaaggcgatc ttgtgctact
    16741 acatagccat agccataaaa taggtgccat gtatcattgg cataaatatg cggcatgccg
    16801 tattcatcgc gaacaatctt tatctcactt gacgactgct cagccagtgc aggtaagctc
    16861 caataataca tcagggaagc agtaacacag ttcacgatca tacgatttct atttttcatt
    16921 gtatcctctg gcaggtgtat aattagcgaa ttgatactag caacgaagct tcggttatct
    16981 atttgcagag aattgtttca ttatgaactg tgagcgcgat aactaatttt tgatacaaaa
    17041 attttattaa tgtgatctaa aacgaatcgc tttattcacc gctgatgatt ttgttctatt
    17101 cctttttgtg acagatcccc cggacgcata gatgatgacc cc
//
LOCUS       DAZUGK010000094        17125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZUGK010000094 DAZUGK010000000
VERSION     DAZUGK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.094"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..217
                     /locus_tag="ACHY5W_002679"
     CDS             <1..217
                     /locus_tag="ACHY5W_002679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447557.1"
                     /translation="IFLWLKANVELSIKFTSNCNYILYETQEEIIIYRDDTRRKKNNN
                     LHETHPDGSIVNSPITGIPPAFYGCFH"
     gene            361..1704
                     /locus_tag="ACHY5W_002680"
     CDS             361..1704
                     /locus_tag="ACHY5W_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447558.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNGPHEYAKFTLEHIRQWAALDKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1853..3361)
                     /gene="putP"
                     /locus_tag="ACHY5W_002681"
     CDS             complement(1853..3361)
                     /gene="putP"
                     /locus_tag="ACHY5W_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFS4447559.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGSVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3520..3729)
                     /locus_tag="ACHY5W_002682"
     CDS             complement(3520..3729)
                     /locus_tag="ACHY5W_002682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447560.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3784..7746
                     /gene="putA"
                     /locus_tag="ACHY5W_002683"
     CDS             3784..7746
                     /gene="putA"
                     /locus_tag="ACHY5W_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4447561.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSLVLWPDDALHRQLVKALPSAVSERIQLAKAENIIAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7786..8424)
                     /gene="rutR"
                     /locus_tag="ACHY5W_002684"
     CDS             complement(7786..8424)
                     /gene="rutR"
                     /locus_tag="ACHY5W_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFS4447562.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQQIIIEGIRPR"
     gene            8712..9802
                     /gene="rutA"
                     /locus_tag="ACHY5W_002685"
                     /pseudo
     CDS             8712..9802
                     /gene="rutA"
                     /locus_tag="ACHY5W_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
     gene            9802..10494
                     /gene="rutB"
                     /locus_tag="ACHY5W_002686"
     CDS             9802..10494
                     /gene="rutB"
                     /locus_tag="ACHY5W_002686"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFS4447563.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10506..10892
                     /gene="rutC"
                     /locus_tag="ACHY5W_002687"
     CDS             10506..10892
                     /gene="rutC"
                     /locus_tag="ACHY5W_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFS4447564.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10900..11700
                     /gene="rutD"
                     /locus_tag="ACHY5W_002688"
     CDS             10900..11700
                     /gene="rutD"
                     /locus_tag="ACHY5W_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFS4447565.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11710..12300
                     /gene="rutE"
                     /locus_tag="ACHY5W_002689"
     CDS             11710..12300
                     /gene="rutE"
                     /locus_tag="ACHY5W_002689"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFS4447566.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12311..12805
                     /gene="rutF"
                     /locus_tag="ACHY5W_002690"
     CDS             12311..12805
                     /gene="rutF"
                     /locus_tag="ACHY5W_002690"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFS4447567.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12826..14154
                     /gene="rutG"
                     /locus_tag="ACHY5W_002691"
     CDS             12826..14154
                     /gene="rutG"
                     /locus_tag="ACHY5W_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFS4447568.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLLFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYVVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGRLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14237..14410)
                     /gene="ymdF"
                     /locus_tag="ACHY5W_002692"
     CDS             complement(14237..14410)
                     /gene="ymdF"
                     /locus_tag="ACHY5W_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS4447569.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14783..15379
                     /gene="wrbA"
                     /locus_tag="ACHY5W_002693"
     CDS             14783..15379
                     /gene="wrbA"
                     /locus_tag="ACHY5W_002693"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS4447570.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVGGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15400..15627
                     /gene="yccJ"
                     /locus_tag="ACHY5W_002694"
     CDS             15400..15627
                     /gene="yccJ"
                     /locus_tag="ACHY5W_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFS4447571.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15665..16906)
                     /gene="agp"
                     /locus_tag="ACHY5W_002695"
     CDS             complement(15665..16906)
                     /gene="agp"
                     /locus_tag="ACHY5W_002695"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFS4447572.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEKAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
BASE COUNT         4039 a         4553 c         4534 g         3999 t
ORIGIN      
        1 aatttttctc tggctgaagg ctaacgtcga gttatcgata aaattcacga gtaattgtaa
       61 ctatatactt tatgaaacac aggaagaaat tattatttac cgcgatgata caaggcgtaa
      121 aaaaaataat aatcttcatg aaactcaccc cgatggcagc attgtaaatt cgccaataac
      181 tggcattcct cctgcttttt acgggtgttt ccattaacac cagaatgagc ggattgttta
      241 ctacatcaag aacgcatccg aagtgtacat cctggtgcgc cgactgaatc gcccggagcc
      301 atgcggatgg ctacgactta atacgaatca ttaaccctgt aaggatacgg agagcatgac
      361 atgagaccaa tatccaacct caatacgagc gcagcaacct atattccacc gcaacaactc
      421 ccgagtagtt gtctggaaac tttgacgctt ctggtgcaga tgaataatta tatcaaaagg
      481 gatgcggact acagcacggg catggctgtt actccactgg ttcctgaaga ggttcacttg
      541 ctggctgttg cgatgacaac cgaactacat cgacatcagt tccaaccggt ggtaagtgca
      601 aatgatctgc aacttcctga accgttcacc ttcgatgttg ccggtttcac catcaccttc
      661 acgaagacgc aagagcatga caacacgggc aatctcatga aaattgcagt cagcaagagc
      721 ggtgtgtgca ccagtacaaa tattactctt gagctgtttc actccattgt cacaaccctg
      781 atgtcacgca gtcaatatgg aacctttgat ctctggtccg tacggccaat actcacggat
      841 gaaagtcaaa acagggtgca tgaagcagct cgctactccc cggcccagca atacggaaga
      901 gaagaaacac actttaccaa caatggcatg cgagaatttg gaaatatgtc tccgctggcg
      961 tggcgtaacg atgtggaatt acagcaatcg tgtaatacaa gcaacatccc accaaatgct
     1021 gcaaatgacg acataaacaa caccaggcag accatcgagg accagccaga ggcggacgag
     1081 ccacagcaat atgttaaatt gactgtagac gacatgcgaa aatgggcggc tatggaccag
     1141 caggcgcgaa acgctctaca gggagtttca ggatggtgta cgcgcaatca ttttaatatc
     1201 aaaaatgcca gaaattacct gaccgatcac ggcctgaact acgcgggaca ggtaaaggta
     1261 aacgggccgc atgaatatgc caaattcacc cttgaacata tccgccagtg ggcagcctta
     1321 gacaagcatg tgcgaaaacc agttggttat ttagaaaagt ggtgtaagga aagaaacctc
     1381 gcgccaacta ctgccaggaa ttacctgaag aacgacgggc ttaccgccct gggtgagttg
     1441 aagctgacag ggccgcagaa atgggtcacg ttcaccttcg gagacatcgt acaatgggcg
     1501 aatatgaccc aggaggagcg taacagcgca gggggagccg aaaagtggag taaaaaacgc
     1561 ggcttccagt ggtctactgc cagatcctac ctgaaatcga gcggagtgac caagcaaggt
     1621 gcgagaaaac tggcgtggtt aaaaaacagc gggaatatgt cgaatccatt ctatctcgcc
     1681 caaccaacca gccgcaggac ataagctgaa ggtaccgata gcggcaattt tgttcatctg
     1741 tgccggctgg gcaaatagca attgcccccg aacactccct gccaggcaac ttataccgca
     1801 tggcagggaa cattcgggtt aaaaagcaaa agataaataa ttactcttca gcttagcctt
     1861 cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc agcctcggca aagcgttttt
     1921 gcatcgccgc tgacggcgct ttacccagca aactaaacac tacaatccca atactgccga
     1981 agataaagcc cggaataatt tcgtacagtc ccagccagcc gaactgtttc cagacgataa
     2041 ccgtcagcgc cccgatgatc atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca
     2101 tcaccgagaa cagcaccacc gaaccaaacg ccgcgccaaa gcctgcccac gcgtagctca
     2161 ctaagcccag cacgcggttt tccgggtttg ccgccagcgc aatggccacc agcgccacca
     2221 ccagcaccat cacacgccct acccacacca gctctttctg gctggcatgt ttacgcagaa
     2281 acgctttgta taaatcttcg gtaatcgcac tggagcacac cagcagctgg caacttaagg
     2341 ttgacattac cgccgccaga attgccgaca gcagaatccc ggcaatccac gggttaaaca
     2401 gaatttgcgc cagttcgata aacacgcgtt cagcgttctg gtttaccgca cctgctaccg
     2461 ccgggtgctc gttaaagtaa gcgataccaa agaagccgac agccaccgcc cctgccaggc
     2521 agaggatcat ccaggtcata ctgatacgac gcgcatggac gatgctgtgg tgagaatccg
     2581 ccgccataaa acgcgccagg atgtgcggtt gcccgaagta acccagcccc caccccatca
     2641 gcgagataat ggcgacaaag ttcagccctt tgagcatatc cacgttttcg atgctctttt
     2701 gtttgatcac ttccagcgag tcaccaaagc caccgacact gataatgacg ataaccggcg
     2761 tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag ctcaccgcga
     2821 ggaaaccgcc aataaaggtg taaaggatcg tcgccgcagc tcccgcccac agagccgttt
     2881 cgtagctcat gccaaaggta ctttcaaaca gacgcgcgcc tgccacaatg cccgaagcgc
     2941 aataaatggt gaagaacagc aaaataacca gcgcggagat aatgcgcaaa atgcggcttt
     3001 tatcttcaaa acgcccggtg aaataatccg gcaatgttaa ggcgttattg ttgtattcgg
     3061 tatgcacacg caaccgcccg gccaccagct tccagttaat ccacgcgcct aatgtcaggc
     3121 caatggcgat ccaactttcg gaaatcccgg aaagaaaaac cgcgcccggc aaccccatta
     3181 acagccagcc gctcatatcc gatgcacccg ccgataatgc cgtcacgaat ggcccaaggc
     3241 tacgtccgcc cagaatatag tcgtcaaagt ttttcgttga tcgccaggcg ataaacccaa
     3301 tcaatatcat gccaaagata tagacacaaa atgtcaccaa catcggtgtg ctaatagcca
     3361 tctaaagtct ccaaaaaatt attatcggca atgtcgaaac ttgccgttat atctgccacc
     3421 ggaacggggt aacagagttt atgttttacc agggcgaccg tatcctgccg gaagcgctgg
     3481 ttattcacaa tcgatttaac acaccattta cattaaattt tagtgctcag cgacactatt
     3541 tttcatcagg ttgcactctc tcacattttt tgcggttgca cctttcaaaa atgttaactg
     3601 ccgcagagaa aaagtctgag ttattttttt aatccctgtc atatcgattt cttttattaa
     3661 catttcattc atttttaagc ttgctacgca ggtcacattt aacatggttg cacaaagttg
     3721 caacatcatg gatatttcac gataacgtta agttgcacct ttcagaacaa caggagtaat
     3781 ggcatgggaa ccaccaccat gggggttaag ctggacgacg cgacgcgtga gcgtattaag
     3841 tctgccgcga cacgtatcga tcgcacgcca cactggttaa ttaagcaggc gattttttct
     3901 tatcttgaac aactggaaaa cagcgatact ctgccggagc tacctgcgct gctttctggc
     3961 gctgccaatg agagcgatga agcaccgact ccggcagagg aaccacacca gccattcctc
     4021 gactttgccg agcaaatttt gccccagtcg gtttcccgcg ccgcgatcac cgcggcctat
     4081 cgccgcccgg aaaccgaagc ggtttctatg ctgctggaac aagcccgcct gccgcagcca
     4141 gttgctgaac aggcacacaa actggcgtat caactggccg ataaactgcg taatcaaaaa
     4201 aatgccagtg gccgcgcagg tatggtccag gggttattgc aggagttttc gctgtcatcg
     4261 caggaaggcg tggcgctgat gtgtctggca gaagcgttgt tgcgtattcc cgacaaagcc
     4321 acccgtgatg cgttaattcg cgacaaaatc agcaacggta actggcagtc acacattggt
     4381 cgtagcccgt cactgtttgt taatgccgct acctgggggc tgttgtttac tggcaaactg
     4441 gtttccaccc ataacgaagc cagcctctcc cgctcgctga accgcattat cggtaagagc
     4501 ggtgaaccgt tgatccgcaa aggtgtggat atggcgatgc gcctgatggg agagcagttc
     4561 gtcactggcg aaaccattgc ggaagcgtta gccaatgccc gcaagctgga agagaaaggt
     4621 ttccgttatt cttacgatat gcttggcgaa gctgcactaa ctgccgcaga tgcacaagcg
     4681 tatatggttt cctatcagca ggcgattcac gccattggta aagcgtccaa tggtcgtggc
     4741 atctatgaag gtccgggcat ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc
     4801 gcccagtatg accgggtaat ggaagagctt tacccgcgtc tgaaatcact caccctgctg
     4861 gcgcgtcagt acgatattgg tatcaacatt gacgccgaag aggccgatcg tctggagatc
     4921 tccctcgatc tactggaaaa actctgtttc gagccggaac tggcaggctg gaacggcatc
     4981 ggttttgtta ttcaggctta tcaaaaacgc tgcccgttgg tgattgatta cctgattgat
     5041 ctcgccaccc gcagccgtcg ccgtctgatg attcgcctgg tgaaaggcgc gtactgggat
     5101 agtgaaatca agcgtgcgca gatggacggc cttgaaggtt atccggttta tacccgcaag
     5161 gtgtataccg acgtttctta tctcgcctgt gcgaaaaagc tgctggcggt gccgaatcta
     5221 atctacccgc agttcgcgac gcacaacgcc catacgctgg cggcaattta tcaactggca
     5281 ggtcagaact actatccggg tcagtacgag ttccagtgcc tgcatggtat gggcgagcca
     5341 ctgtatgagc aggtcaccgg gaaagttgcc gacggcaaac ttaaccgtcc gtgtcgtatt
     5401 tatgccccgg ttggcacaca tgaaacgctg ctggcgtatc tggtgcgtcg cctgctggaa
     5461 aacggtgcta acacctcgtt tgttaaccgt attgccgaca cctctttgcc actggatgaa
     5521 ctggtcgccg atccggtcac tgctgtagaa aaactggcgc aacaggaagg gcaaactgga
     5581 ttaccgcatc cgaaaattcc cctgccgcgc gatctttacg gtcacgggcg cgacaactcg
     5641 gcagggctgg atctcgctaa cgaacaccgt ctggcctcgc tctcttctgc cctgctcaat
     5701 agtgcactgc aaaaatggca ggccttgcca atgctggaac aaccggtagc ggcaggtgag
     5761 atgtcgcccg ttattaaccc tgcggaaccg aaagatattg tgggctatgt gcgtgaagcc
     5821 acgccgcgtg aagtagaaca ggcgctggaa agtgcggtta ataacgcgcc aatctggttt
     5881 gccactcctc cggctgaacg cgcggcaatt ttacatcgcg ctgccgtgct aatggaaagc
     5941 cagatgcagc aactgattgg tattctggtg cgtgaagccg gaaaaacctt cagtaacgcc
     6001 attgccgaag tgcgcgaagc ggtcgatttt ctccactact acgccggaca ggtgcgggat
     6061 gatttcgcta acgaaaccca tcgtccatta gggcctgtgg tatgtatcag cccgtggaac
     6121 ttcccgctgg cgattttcac cgggcagatt gctgccgcac tggcggcagg taacagcgtg
     6181 ctggcaaaac cggcagaaca aacgccgctg attgccgcgc aagggatcgc cattttgctg
     6241 gaagcgggtg taccgccagg cgtggtgcag ttgctgccag gtcaggggga aaccgtgggc
     6301 gcgcaactga cgggtgatga tcgcgtgcgc ggggtgatgt ttaccggttc aaccgaagtc
     6361 gctacgttac tgcagcgcaa tatcgccagc cgcctggacg ctcagggtcg ccctattccg
     6421 ctcatcgctg aaaccggcgg catgaacgcg atgattgtcg attcttcagc actgaccgaa
     6481 caagtagtaa tagatgtgct ggcctcggcg ttcgacagtg cgggtcagcg ttgttcggca
     6541 ctgcgcgtgc tgtgcctgca agatgagatt gccgaccaca cgttgaaaat gctgcgcggc
     6601 gcaatggccg aatgtcggat gggtaatccg ggtcgcctga ccactgatat cggtccggtg
     6661 attgatagcg aagcgaaagc caatatcgag cgtcatattc agaccatgcg tagcaaaggc
     6721 cgtccggtgt tccaggcggt acgggaaaac aacgaagatg cccgtgaatg gcaaagcggc
     6781 acctttgtcg ccccgacgct gatcgaactg gatgactttg ccgaattgca aaaagaggtc
     6841 tttggtccgg tgctgcatgt ggtgcgttat aaccgtaatc agctgccgga gctgatcgag
     6901 cagattaacg cttccggcta tggtctgacg cttggcgttc atacgcgtat tgatgaaacc
     6961 atcgcccagg tcactggctc agcccatgtt ggtaacctgt atgttaaccg taatatggtg
     7021 ggcgcagtgg ttggggtgca gccattcggc ggcgaagggt tgtccggtac cgggccgaaa
     7081 gcaggcggtc cgctctatct ctaccgtctg ctggcgaatc gcccggaaag tgcgctggcg
     7141 gtgacgctcg cgcgtcagga tgcagagtat ccggtcgatg cgcagttgaa agccgcattg
     7201 actcagccgc taaatgcact gcgggaatgg gcagcaaatc gtccagaatt gcaggcgtta
     7261 tgtacgcaat atggcgagct ggcgcaggca ggaacacaac gattgctgcc agggccgacg
     7321 ggtgaacgca acacctggac gctgctgccg cgtgagcgcg tattgtgtat cgcggatgat
     7381 gagcaggatg cgctgactca gctcgccgcc gtgctggcgg tgggcagcct ggtgctgtgg
     7441 ccggatgacg cgctgcatcg tcagttagtg aaggcattgc catcggcagt cagcgaacgt
     7501 attcaactgg cgaaagcgga aaatataatt gctcaaccgt ttgatgcggt gatcttccac
     7561 ggtgattcgg atcagcttcg cgcattgtgt gaagcagttg ccgcgcggga tggcgcaatt
     7621 gtttcggtgc agggttttgc ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc
     7681 gagcgttcgc tgagtgtgaa taccgctgcc gctggcggta acgccagctt aatgactata
     7741 ggttaaacag gtagccggag gatgttacaa cctcctccgg catctttaac gtggtcgaat
     7801 cccctcaata ataatctgct gcacgttttc aaccgtttga ttgaaaaata cctcatcgcg
     7861 caacgttgcg cctgtcactg cctccacctg aggggcgaaa tcggcgtaat gttgagtgga
     7921 agcccaaatc ataaaaatca aatgctgcgg atcaatcggc gcgagtttgc cgcttttgac
     7981 ccaaccggca atcagcgccg atttctcatc aattaatgcc ttcaaatcgc ccgtcagttc
     8041 atccattaac agcggcgcgc ctgccagcat ctccatacag aacaggcgcg aagcctgcgg
     8101 ataatcgcgt gagacttcca gcttcagacg gatgtactct ttgatcgccg ccagcggggc
     8161 gaaatcttca cgaaacgctt ttaacggtgc cagccagata tcgagaatct gccgcagcac
     8221 ggcaatatac agcgcctctt ttgacggaaa gtaatacagc agattggttt ttgaaacacc
     8281 cgccaactct gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc
     8341 cagtgctgcg ctaagaatcg cttttttctt cgcactgact gcgcgcgaac gtttacccgt
     8401 tgttttcact gcaccttgcg tcatgcgctc tcccctcttg tagattggca acaggatagc
     8461 aaaacccccg cccggcctcg accttctgca ctctcatcgc gcacagatgc atgttttatg
     8521 tgcaactgtt ttgaccgttt agtccacttt ttaccagaca ttttagataa cgcgatgtta
     8581 acacattaat aacactccct ttttaaaaac tggcatccac tttgcaaaca agccattacg
     8641 cggctgaaaa ggatatgcag gacgcagtgc cccgtctgac tttcacttta cgagacgaag
     8701 agaggttgat gatgaaaatt ggcgtattcg tacctattgg caacaacggc tggctcattt
     8761 cgacccacgc gccgcagtac atgccgacct ttgaactgaa taaatccatt gtgcaaaaag
     8821 cggagcacta ccatttcgat ttcgccctgt cgatgatcaa actgcgtggc tttggcggca
     8881 aaactgagtt ctgggatcac aaccttgagt cgttcacctt gatggcgggg ctggcggccg
     8941 tgacctcgcg cattcagatt tacgccaccg ccgccacctt aacgttacct ccagcaatcg
     9001 tcgcccgtat ggccgcaacc atcgactcca tctctggcgg gcgttttggc gtcaacctcg
     9061 tgactggctg gcaaaagccc gagtatgagc agatgggtat ctggcctggc gatgactatt
     9121 tctcccgtcg ttacgactat ctcaccgaat atgttcaggt gctgcgcgac ctgtggggct
     9181 cggggaaaag cgattttaaa ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc
     9241 aaccgagtgt acccatgaaa gtgatctgcg ccgggcaaag cgacgctggc atggcgttct
     9301 ccgcccagta tgccgatttc aacttctgtt tcggcaaagg cgtaaataca cccacggctt
     9361 tcgccccgac cgctgcgcgg atgaaacagg ccgcagagca aaccgggcgc gacgttggtt
     9421 cttatgtatt gtttatggtg attgccgatg aaaccgacga tgccgctcgc gccaaatggg
     9481 aacactacaa agcgggcgcg gatgaagagg cgttaagctg gctaaccgaa caaagtcaga
     9541 aagatacccg ctccggtact gacaccaacg tccgtcagat ggccgatccc acttcggcgg
     9601 taaacatcaa tatggggacg ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg
     9661 aagtcgcaag cgtgcctggt gcgaaggcgt gctgttaacc ttcgacgatt ttctgtcggg
     9721 aatcgaaacc ttcggcgagc gcattcaacc actgatgcag tgccgcgccc atctccctgt
     9781 gctgactcag gaggtggcat gatgacgaca ttaaccgctc gaccggaagc cattaccttc
     9841 gatccgcagc aaagtgcgct gatcgtggtg gatatgcaaa acgcctatgc cacgccaggc
     9901 ggctacttag atctcgccgg gtttgatgtc tcaaccactc gcccggtcat tgccaacatt
     9961 caaaccgccg tgaccgcagc gcgagcggca gggatgttga tcatctggtt tcaaaatggc
    10021 tgggatgaac agtatgtcga ggctggcggc cccggctcac cgaattttca taaatcgaac
    10081 gccttgaaaa ccatgcgtaa gcagccgcag ctacagggta aattgctggc gaaaggctcc
    10141 tgggattatc aactggtgga tgaactggtg ccacaacctg gtgatattgt gctgccgaag
    10201 ccgcgctaca gcggtttctt caatacgccg ctggacagca ttttgcgcag ccgcggaata
    10261 cgccatctgg ttttcaccgg catcgctacc aacgtctgcg tcgaatcgac gctacgcgac
    10321 ggcttttttc tggagtattt cggcgtggtg ctggaagacg caactcacca ggcggggccg
    10381 gaatttgcac agaaagctgc gttgttcaat atcgaaacct tttttggctg ggtcagcgac
    10441 gtcgaaacgt tctgcgacgc gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt
    10501 taacgatgcc aaaatccgta attattcccg ctggcagcag cgcaccgctg gcccccttcg
    10561 ttcccggcac gctggctgat ggcgtggtgt atgtctccgg tacgctggct tttgatcaac
    10621 ataataacgt gctgtttgcc gatgacccaa aggcgcaaac ccgccacgtt ctggaaacta
    10681 tccgcaaggt gatcgagacg gcgggtggca cgatggcgga tgtgaccttc aacagcatct
    10741 ttattaccga ctggaaaaat tacgccgcga ttaacgaaat ctacgccgag ttttttccgg
    10801 gtgataaacc ggcgcgattc tgcattcagt gcggactggt aaaacctgac gcgctggtgg
    10861 aaatcgccac aattgcgcat atcgccaagt gaggccgcga tgaaactttc actctcacct
    10921 cccccttatg ctgatgcgcc cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt
    10981 tactggttac cgcaactggc ggtgctggag caggagtatc aggtagtctg ttacgaccag
    11041 cgcggcaccg gcaataatcc cgacacgctg gcagaagatt acagtatcgc ccagatggca
    11101 gcggaactgc atcaggcgct ggtagccgca gggattgagc gttacgcggt ggtcggccat
    11161 gcgctcggtg cgctggtggg gatgcagctg gcgctggatt atcccgcgtc ggtaactatg
    11221 ctggtcagcg ttaacggctg gctacgaata aacgcccata cgcgccgctg ttttcaggtt
    11281 cgcgaacgat tactgtatag cggtggcgcg caggcatggg tggaagcgca gccgttgttc
    11341 ctctatcccg ccgactggat ggcggcccgc gcacctcgcc ttgaggcaga agacgcgctg
    11401 gcactggcgc attttcaggg caaaaataat ttactgcgtc gacttaacgc cctcaaacgc
    11461 gctgacttta gtcaccatgc ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt
    11521 gatgatctgc tggtgccatc agcatgttcc agtgaacttc atgccgccct gcccgatagc
    11581 cagaaaatgg tgatgcgcta tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt
    11641 aatgctctgt tactcaacgg gcttgccagc ctgttacatc accgtgaagc cgccctgtaa
    11701 ggaattgcta tgaacgaagc cgttagccca ggtgcgctta gcaccctgtt caccgatgcc
    11761 cgcactcaca acggctggcg ggagaccccc gtcagcgatg agacgttacg ggagctttat
    11821 gccctgatga aatgggggcc gacatcagct aactgttctc cggcacgtat cgtgtttatc
    11881 cgcactgcag agggaaaaga acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa
    11941 accctgaccg cgcccgtcac cgctatcgtc gcatgggaca gtgaatttta tgaacggtta
    12001 ccgcaactgt ttcctcacgg cgatgcccgc agttggttta cctccagccc acaacttgcc
    12061 gaagaaacag cgtttcgcaa cagttccatg caggcggctt atctgatcat cgcctgccgt
    12121 gcgctggggc tggataccgg cccgatgtcg ggttttgatc gtcaacacgt agacgacgct
    12181 ttttttgcag gcagcacgct gaagagcaat ctgctgatta atatcggcta tggcgatagc
    12241 agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa
    12301 ggagcaaact atgaacattg tcgatcaaca aacttttcgc gatgcgatgt cctgcatggg
    12361 cgcggcggtc aatatcatca ccacggacgg tccagccggg cgcgccgggt tcaccgccag
    12421 cgccgtctgc agcgtgaccg acacgcctcc cacattactg gtgtgcctga atcgtggggc
    12481 gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt gtaaatacac ttagcgccgg
    12541 gcaggagccg ctttcaaacc tttttggcgg caaaacgccc atggaacacc gctttgccgc
    12601 cgcccgctgg cagactggtg tgaccggatg cccgcaactg gaagaggcgc tggtttcgtt
    12661 tgactgccgt atcagccagg tggtgagcgt cggcacccac gacattttgt tttgcgccat
    12721 cgaagcgatt catcgtcacg ccacccctta cgggctggtg tggttcgatc gcagttatca
    12781 cgcgctgatg cgccctgctt gttaacctcc ttaaggagac agctgatggc aatgttcggt
    12841 cttcctcact ggcagttaaa atcgacctct acagagagcg gcgtggtcgc gccggatgaa
    12901 cgattgctgt ttgctcagac ggcaataatg ggcgttcaac acgcggtggc gatgtttggc
    12961 gcgacggtgt taatgccgat tctgatgggg ctggatccca atctttccat tttgatgtcg
    13021 gggatcggca cgttactgtt tttctttatt accggcgggc gcgtccccag ttatctcggc
    13081 tccagcgcgg cttttgtcgg cgtggtgatc gccgcaacgg gctttaacgg tcagggcatg
    13141 aacccaaata tcagcatcgc gctgggcggg attatcgcct gtgggctggt ttataccgtc
    13201 attggtctgg tggtgatgaa aatcggtacg cgctggattg aacggctaat gccgccggtg
    13261 gtgacgggtg cggtggtgat ggcgattggc ctgaacttag cgcctatcgc ggtaaaaagt
    13321 gtatcggctt caggctttga tagctggatg gcggtaatga cggtgctgtg tattgggctg
    13381 gtggcggtgt ttacccgtgg catgatccag cggttattga ttcttgtagg attaattgtc
    13441 gcctgtctgc tgtatgtcgt gatgaccaac gttttagggc tgggaaaagc cgtcgatttt
    13501 actctcgtca gccatgctgc ctggttcggt ctgccgcatt tttccactcc tgcttttaat
    13561 agtcaggcaa tgatgctgat tgcgcccgtg gcggtgattc tggtggcgga aaacttaggc
    13621 cgtctcaagg cggtcgccgg gatgaccgga cgcaatatgg atccgtatat gggacgggca
    13681 ttcgtcggtg atgggctggc gacgatgctt tctggttctg tcggtggcag cggcgtaacc
    13741 acctacgccg agaatatcgg tgtaatggcc gtgacaaaag tctactcgac gctggtgttt
    13801 gtcgctgctg ccgtcatcgc catgctgttg ggcttttcac cgaaatttgg cgcgctgatt
    13861 cataccattc cggccgcggt gattggtggt gcatcaattg tagtgttcgg gctgattgcc
    13921 gtcgcaggcg cacggatctg ggtacaaaac cgtgtcgatt taagccagaa cggtaatttg
    13981 attatggtcg cagtaacctt agtgctgggc gcgggtgatt ttgctctcac gctgggcggt
    14041 tttacgttgg gagggatcgg tacagcaacc tttggcgcga ttttactcaa tgcgttatta
    14101 agcagaaggt tggttgacgt tccaccgcca gaagttgttc atcaggagcc ataaacagaa
    14161 aaccgccggg attaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgcattcg
    14221 tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac
    14281 ctgcttcaga tgcgcgctgc gggtcattct tgaaattacc cccgctgtgc tgtccacctt
    14341 ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat
    14401 ggtttgccat tactgacctc cgtctgggta tagacatcat attgcataga attaacgcgg
    14461 cgtatggcgg ctgccatatt ttcgctacgt gggactaagc ttagtgaact gacgcgggca
    14521 gacccgcgtt tggtaatatc ctgcaacagc ttcttagcga gaatagatat catctccaat
    14581 aattatccat aagccgctct tatgattata aaaacgcgat gagataaaaa tgtgtcattt
    14641 tgcgacaaaa ttgcgtgctt gtgaaagttg ttataaatca aataagtggt tgtgaaattt
    14701 gcactctgaa aaggacgtct tatctttaaa taagtggtag cgaatcgcta cggaatagag
    14761 ataacacgag gagtggttag aaatggctaa agttctggtg ctttattatt ccatgtacgg
    14821 acatattgaa acgatggcac gcgcagtcgc tgagggtgca agcaaagtgg gtggcgctga
    14881 agttgtcgtt aagcgtgtac cggaaaccat gccgccgcaa ttatttgaaa aagcaggcgg
    14941 taaaacgcaa actgcaccgg ttgcaacccc gcaagaactg gccgattacg acgccattat
    15001 ttttggtaca cctacccgct ttggcaacat gtccggtcaa atgcgtacct tcctcgacca
    15061 gacgggcggc ctgtgggctt ccggcgcact atacggaaaa ctggcgagcg tctttagttc
    15121 caccggtact ggcggcggtc aggaacaaac catcacatcc acctggacga cccttgcgca
    15181 tcacggcatg gtgattgtcc ccattggcta cgcagcgcag gaattatttg acgtttcaca
    15241 ggttcgcggc ggtacgccgt acggcgcaac caccatcgca ggcggtgacg gttcacgcca
    15301 gcccagccag gaagaactgt ctattgctcg ttatcaaggg gaatatgtcg caggtctggc
    15361 agttaaactt aatggctaat cttcaacagg aggatacgca tgccaactca agaagcgaaa
    15421 gctcatcacg tcggtgaatg ggcatctctg cgcaatacgt cgccggaaat agccgaggcc
    15481 attttcgaag tcgccgggta cgacgaaaaa atggcggaaa agatttggga agaaggtagt
    15541 gatgaagtgt tagtcaaagc ctttgctaag accgataaag attcgctttt ttggggcgaa
    15601 cagaccatcg aacgtaaaaa cgtttaacca gcgactcccc cgcttctcgc gggggagttt
    15661 tctgttattt caccgcttca ttcaacacgc tatcaaactt atccatcggg cagaaaccat
    15721 cagcgtctat cgggcaaccg cttaattcca gcgtcacgcg ctgagcaggt gcctgaaggg
    15781 ttaacgcatc ggcattacgt aactgttccg cactctggta cacatactcg attttcatca
    15841 aatcgcgatt ggctttgctg tcatgccaac gctggaaaac gattttgccg ccaatcggcg
    15901 tgcgttcgtt ctggtcatgc aactgatacg gtttgaaatc cagcgccgtt aacagagagg
    15961 ctatgttgga gtcgtgccca actaacaccg tgattttcgg agcactggcg cgatcggtga
    16021 ccagtgcttt gtcgatataa ctgaccagcg gtttcgcaac attgcgcgcc acttccggtg
    16081 aggtaaacag gctgtcctgg taaccgtttt tcagcttcga caacaccttc cactgctggt
    16141 cagatttgat ttctccccag gccacctgat ccatcggaaa accttcgtag tattgcaaag
    16201 taaacgcatc taccagcgag ttgccgactt tcagcggccc ggaaacacct ggttcttgtt
    16261 gatacttcgc gctaaaggta tttttgccat ccaccagcga acactgttgt ttctctttac
    16321 aggcagggga atctttatag ttaacgattt tttccagtag ctggtagctg tcggtaagct
    16381 ggagtttgct gagctctttc tccattgccg ccaccgcctt ttcactgaat gcggcggaat
    16441 catcggtgat caccgggtta aaggttgggt ccatggtgcc cattttttcc tggtgatgca
    16501 caggaatatc acaccccggg aatgcgccgg taataaagaa ctgtgcagtc gcaacggtac
    16561 gttgcagact attggcatag gcatatacgg tgtccggcgg cgggcattca cccgatttta
    16621 ccatcccctg ctctgccagc cattcacgca tgtaatggcc catatacact tcgagcacgc
    16681 cacctttggt ggtgagttgc ccaccgggga cgtcccattc tggccattta ttcggcgtcg
    16741 actgctccag cacactgcca ttgttcgcca gcggcgcacg taagttatgg cggctcatca
    16801 tgagcacttg ctgtagctga tagccttccg gtacggtttg tgcctgagca tttgaagcga
    16861 gtaaaactat ccctgccaca gttgcggtga ttagcgtttt gttcattcct ggcacctctt
    16921 ttgttatcaa tcactaaaga gtgtgacaga aatatgactt ttttccggga acaggttcgc
    16981 aaaatggctt gttgaacgcc tgtttttcta tatacatctt caaaaacagg caaggtttga
    17041 cgcaaactat gcctgattag cccctgcctc gcgtacgccg ctgataattg cacctgtggc
    17101 taaacagaat atctgcggcg ttacc
//
LOCUS       DAZUGK010000095        17115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZUGK010000095 DAZUGK010000000
VERSION     DAZUGK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.095"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..529)
                     /locus_tag="ACHY5W_002696"
     CDS             complement(<1..529)
                     /locus_tag="ACHY5W_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4447573.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQE"
     gene            1007..1795
                     /gene="fabI"
                     /locus_tag="ACHY5W_002697"
     CDS             1007..1795
                     /gene="fabI"
                     /locus_tag="ACHY5W_002697"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4447574.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1939..3066
                     /gene="yciW"
                     /locus_tag="ACHY5W_002698"
     CDS             1939..3066
                     /gene="yciW"
                     /locus_tag="ACHY5W_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFS4447575.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRVNTQPNNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3134..5068
                     /gene="rnb"
                     /locus_tag="ACHY5W_002699"
     CDS             3134..5068
                     /gene="rnb"
                     /locus_tag="ACHY5W_002699"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFS4447576.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFSA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5146..5249)
                     /locus_tag="ACHY5W_002700"
                     /pseudo
     CDS             complement(5146..5249)
                     /locus_tag="ACHY5W_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5303..7288
                     /gene="pdeR"
                     /locus_tag="ACHY5W_002701"
     CDS             5303..7288
                     /gene="pdeR"
                     /locus_tag="ACHY5W_002701"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS4447577.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQLVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(7270..7398)
                     /gene="ymiD"
                     /locus_tag="ACHY5W_002702"
     CDS             complement(7270..7398)
                     /gene="ymiD"
                     /locus_tag="ACHY5W_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFS4447578.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            7436..7609
                     /gene="yciZ"
                     /locus_tag="ACHY5W_002703"
     CDS             7436..7609
                     /gene="yciZ"
                     /locus_tag="ACHY5W_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFS4447579.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7699..8448
                     /gene="yciT"
                     /locus_tag="ACHY5W_002704"
     CDS             7699..8448
                     /gene="yciT"
                     /locus_tag="ACHY5W_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFS4447580.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            8717..8935
                     /gene="osmB"
                     /locus_tag="ACHY5W_002705"
     CDS             8717..8935
                     /gene="osmB"
                     /locus_tag="ACHY5W_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFS4447581.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9061..9387)
                     /gene="yciH"
                     /locus_tag="ACHY5W_002706"
     CDS             complement(9061..9387)
                     /gene="yciH"
                     /locus_tag="ACHY5W_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFS4447582.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGSVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9387..10124)
                     /gene="pyrF"
                     /locus_tag="ACHY5W_002707"
     CDS             complement(9387..10124)
                     /gene="pyrF"
                     /locus_tag="ACHY5W_002707"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4447583.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(10317..11486)
                     /gene="lapB"
                     /locus_tag="ACHY5W_002708"
     CDS             complement(10317..11486)
                     /gene="lapB"
                     /locus_tag="ACHY5W_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFS4447584.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(11493..11801)
                     /gene="lapA"
                     /locus_tag="ACHY5W_002709"
     CDS             complement(11493..11801)
                     /gene="lapA"
                     /locus_tag="ACHY5W_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFS4447585.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(11950..12714)
                     /gene="pgpB"
                     /locus_tag="ACHY5W_002710"
     CDS             complement(11950..12714)
                     /gene="pgpB"
                     /locus_tag="ACHY5W_002710"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFS4447586.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12884..13474
                     /gene="ribA"
                     /locus_tag="ACHY5W_002711"
     CDS             12884..13474
                     /gene="ribA"
                     /locus_tag="ACHY5W_002711"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFS4447587.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(13538..16213)
                     /gene="acnA"
                     /locus_tag="ACHY5W_002712"
     CDS             complement(13538..16213)
                     /gene="acnA"
                     /locus_tag="ACHY5W_002712"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFS4447588.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(16377..16472)
                     /gene="ymiC"
                     /locus_tag="ACHY5W_002713"
     CDS             complement(16377..16472)
                     /gene="ymiC"
                     /locus_tag="ACHY5W_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFS4447589.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(16586..16753)
                     /gene="yciX"
                     /locus_tag="ACHY5W_002714"
     CDS             complement(16586..16753)
                     /gene="yciX"
                     /locus_tag="ACHY5W_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFS4447590.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(16756..16896)
                     /gene="ymiA"
                     /locus_tag="ACHY5W_002715"
     CDS             complement(16756..16896)
                     /gene="ymiA"
                     /locus_tag="ACHY5W_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFS4447591.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT         4305 a         4389 c         4081 g         4340 t
ORIGIN      
        1 tttcctgcag atgttgtgga tcgctttttt gcggtgttaa acgacgataa accgtacctt
       61 ccatcatggt agcaaacact tcaacgcagc aacgaatgtg ctcgtcgcta agatggggat
      121 gcattctttt catatgtgca caaccgttag cgaacattct ggcgtcagct tcttcaagca
      181 ttgccataac ccgcgggttg cgtgtggctt ctgcagccac ttccagcatc agcgcgtcgt
      241 cgtcctcatt taaggattta cgcaaggcaa ggacttccgg gaagtgatcg gtacgcgcgt
      301 caatgtccat ttgggcaata cgaaagtcga tgatacggcg aaccatctct tcaataatgg
      361 cgtctttgtt ggcgaagtaa cgatatatct gacccacgct aagctgagct tcgctggcaa
      421 tttgcgacat actggcggca tgaaacccgc agcgacgaaa acaccgacgg gcggcgttaa
      481 tgatttcatc ctgacgctgt ttgtggcgta tctccagctt gctggtcatt tctgtctcct
      541 ggcctatcac ttggtgagaa tgatcgttct catttttgct gattatacgt ttctccaaag
      601 taatggaaag tatttttatc ttattcatgg tgcatattct gataatgaag agattgtgga
      661 gattctgtga agatagttga caatattaag ttggggtcaa ttcatcagaa aggctgctac
      721 ttgttgtcgc cagttctgtt ttcatcatca aacttatcgg agctaatacg aatagtttca
      781 tagtcagatt ctataagata tttccccaac ctaccccttg caggcgtcaa gagtagataa
      841 cgcaaaatgc tctgtaccgc agcttctctc cggtacagaa agcgcaacta tagccaccca
      901 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
      961 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
     1021 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
     1081 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
     1141 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
     1201 tgccgaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
     1261 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
     1321 taacgccgtt actcgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
     1381 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
     1441 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
     1501 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
     1561 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
     1621 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
     1681 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctcgg gtgaagtggt
     1741 ccacgttgac ggcggtttca gcattgctgc aatgaacgaa ctcgaactga aataatcgtt
     1801 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
     1861 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
     1921 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
     1981 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
     2041 gtcagcgatc cctttctact cgacgtgata cttgaaaaag aaacgctggc ccccttcctt
     2101 tcatggctgg accctgcgcg tgttcttgca gtggatttgt tccctgacca gcttaccgtg
     2161 acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
     2221 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccggg
     2281 cctgattcca ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
     2341 ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
     2401 cttactgcct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
     2461 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
     2521 gagatacaaa actacgccga cgcgtcactg tttgctgatg aatcattacg ctggcgaagc
     2581 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
     2641 caactggccg aaatactctc tctccaccct atttcacttt cccttctcga aaagttatta
     2701 aacagcacac gggtcaatac acagccgaat aatcagcttg cggcgttgtt atgcgcgcgg
     2761 ataaatggca gtcctgcttg ttttgccacc tgtatggatt catcaaatga atataaaaaa
     2821 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc aatgggctga ccgtcattct
     2881 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
     2941 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
     3001 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
     3061 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
     3121 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
     3181 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
     3241 ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
     3301 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
     3361 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
     3421 tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc gcgcagcccg
     3481 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
     3541 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggtgacga
     3601 ccactttgta ccgtggtggg ttacacttgc gcgccataat ctggaaaaag aagcaccaga
     3661 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga ccgcgctgga
     3721 ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt tcgctaaggc
     3781 gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
     3841 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
     3901 cttcaacatc cctatgcttc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
     3961 tgaagtccgt ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
     4021 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
     4081 ttctgactgg ctggaaaata ccggtgactg gcagcctgaa agtgaagcaa ttgccgaaca
     4141 agtccgtttg ctagcgcaaa tttgccaacg acgcggcgag tggcgtcata accacgcact
     4201 ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
     4261 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgatcgccgc
     4321 taatatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
     4381 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
     4441 gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact
     4501 ggacgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
     4561 aattagcact gaacccggtc ctcactttgg cctcggtctg gaggcatacg ccacctggac
     4621 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
     4681 aggcgaaact gcgacgcgtc cacaggatga aatcactgtt caaatggccg agcgtcgccg
     4741 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
     4801 caaagccggg accgacaccc gtttctcggc ggaaattgtc gatatcagcc gtggcggcat
     4861 gcgtgttcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
     4921 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
     4981 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
     5041 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
     5101 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
     5161 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa aacaatgcgt
     5221 ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc
     5281 agttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt
     5341 tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgttt tgcgcttttc
     5401 taccaccgaa accacagaac ctgatcgtac tttgcagtta tctgccgaac aggctgctcg
     5461 catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgagag
     5521 cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc
     5581 gtctgcatgg catgacacac cggcagttgc tcgtgattta tcacacgggc tttcctttgc
     5641 tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat
     5701 ccaacgcttc aatcggttat gtgaagatta cacagggttg aacgaacacg acgtcattgg
     5761 gcaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa
     5821 ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacacgtaa
     5881 aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga
     5941 gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc aggagcgact
     6001 gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga
     6061 gttaatcgat cacgctatta atcatgcaga taacaataaa gttggggttg tgtatcttga
     6121 tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttgtttggtg accagttatt
     6181 acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggttt tggcgcgtcc
     6241 gggtggcgat gagtttctgg tactggcatc caacacctca caaagcgcgc tggaagcaat
     6301 ggcatcacga attttgaccc gcttacggct cccctttcgc attggtttaa ttgaagttta
     6361 taccagctgt tcagtaggta ttgcactctc tcccgaacat ggttcggaca gcgcggctat
     6421 tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag gacaattttg
     6481 cgtttttacc ccagaaatga atcaacgggt atttgaatat ctctggctgg ataccaactt
     6541 gcgtaaagca ctggaaaacg atcagttggt tattcactat caaccgaaaa tcacctggcg
     6601 tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac gtgggttgat
     6661 tccaccgttg gacttcattt cctacgccga agagtcaggg ctaattgtgc ctttaggccg
     6721 ttgggtgatt ctcgatgtcg tacgccaggt ggcgaagtgg cgggataaag gcattaacct
     6781 gcgagtggcg gtaaacattt ctgcacgtca gctcgccgat caaaccattt tcaccgccct
     6841 gaaacaggtt ctccaggaac tcaattttga atactgccct attgatgttg aattgacaga
     6901 gagttgtctg attgagaatg atgaactggc actgtctgtt attcaacaat ttagccaact
     6961 aggtgcgcaa gtgcatctgg atgattttgg taccggctac tcttcacttt cgcaactggc
     7021 gcgctttccg atcgatgcca tcaaacttga ccaggttttt gttcgagata ttcacaaaca
     7081 acttgtctcg cagtcactgg tccgggcgat cgtcgctgtg gcccaggcat tgaatcttca
     7141 ggtgatcgcc gaaggtgtag agagtgcaaa ggaagatgct tttttaacca aaaacgggat
     7201 caatgagcgg caaggatttt tgtttgccaa accgatgccc gccgtcgcct tcgaacgctg
     7261 gtataaacgc tatctgaagc gcgcataagt agatgaatat atatacttag cgctatattc
     7321 cgtcattata tatcgtattc ccggatgata attagccgtt gctgttaaac acttctcttt
     7381 tagcggcaat ttttgcatta ttcattttcc cccttttgct ataaggatgt ccttgatgtc
     7441 tgaatttgat gcccagcgcg ttgccgaacg tattgatatt gtgttagata tccttgtggc
     7501 aggcgattac cactctgcga tccataatct cgagattctt aaagcagaac tcttgcgcca
     7561 ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg gagatttaac aatcgtgcct
     7621 tcaggttttc ctatttgagg atgcccggcg cgtcagtatt tttttattta gtattataac
     7681 gttataagaa ttacagcgat gaattcccga caacaaacta ttctacagat ggtcattgac
     7741 cagggtcagg ttagcgtaac cgatctggca aaagccactg gagtttctga agttaccatt
     7801 cgccaggatc tcaacaccct cgaaaaactg agttacctcc gccgtgcaca tggctttgca
     7861 gtttcgcttg atagtgatga cgtcgaaacc cgtatgatga gcaattatac gctgaagcgt
     7921 gaactcgccg agtttgccgc gtcactggtt caaccgggcg aaaccatctt tatcgaaaat
     7981 ggcagcagca atgccctgct cgctcggact ctgggcgagc agaagaaaaa tgtcactatc
     8041 atcacggtca gcagctacat cgcgcatttg ctgaaagacg cgccttgtga agttattttg
     8101 ctcggtggcg tgtaccagaa aaaaagcgaa agtatggttg gccctttgac acgccagtgc
     8161 atccaacagg tgcatttcag caaagcattt attggtattg atggctggca acctgaaact
     8221 ggatttaccg gtcgcgatat gatgcgtacc gatgtggtca atgccgtgct ggaaaaagag
     8281 tgcgaagcga tagtcctgac tgacagctcg aaatttggtg ctgtacattc atactccatc
     8341 ggtcccgttg agcgattcaa tcgcgtgatt accgattcga aaatacgcgc cagcgatctg
     8401 atgcatcttg agcacagtaa actcaccgtt cacgtcgttg acatttaacc atttcccttc
     8461 tctacggatg atttgcagtt tggcaaatca tccgctctaa gatgattcct ggttgataat
     8521 taagactatt tacctgttat taacactctc aagatataaa attattatca gcgatataac
     8581 aggaagtcat tatcacctgc gtgatataac cctgcgcgcg agcggatttc acggaataat
     8641 ttcaccaggc ttattcttag ctattatagt tatagagagc ttacttccgt gaatcataaa
     8701 ttcaggagag agtattatgt ttgtaacgag caaaaaaatg accgcggctg ttctggcaat
     8761 taccctggca atgtctctga gtgcctgttc taactggtct aaacgggacc gcaacaccgc
     8821 aatcggcgcg ggtgcagggg cattaggcgg tgcagtactg accgatggca gtacgttggg
     8881 tacattaggt ggcgcagctg tcggtggtgt tattggtcac caggtcggta aataatcgta
     8941 aataaaagtc gattatcgac tcgtaattat aatacgcttg attctgttca gtacgaaata
     9001 actgaataat acacacagta aaatattcga aagccacgga tatatccgtg gctttttatg
     9061 ttaaccgcct gcgagtttta ccttcatccc tttcgcttca agtagtgatt ttaataaatc
     9121 acgcttatcg ccctggattt caataactcc atctttaact gatccgccgc agccgcattt
     9181 tttcttcagt tccgctgcaa gttttgtcag ttcggcatca tcgagatcga taccggtaat
     9241 caggcaaacg cccttacctt tacgtccact ggtctgacgc tgaatacgca ccacaccatc
     9301 gcctttagag cgtactgggg ccgctttggg ctcatcaata cgcccggttt ctgttgagta
     9361 gaccagacgg ctgttggaat cactcatcat gcactccgct gtaaagaggc gttgatcgct
     9421 ttcagcgtct gcgctggatc taccgattgc gttaccgggc gaccaatcac catataatca
     9481 acaccagccg ccaacgcctg ttctggcgtc ataatgcgac gctggtcacc tgcttcactc
     9541 ccctgcggac gaatgcccgg tgtaaccagt ttgaattcct gaccgaatac ctgtttaaag
     9601 cgcacagctt cctgagcaga acataccaca ccatcaaggc cacatttttg cgtcagtgcc
     9661 gccagacgtt ctgcataatc tgcaggtgac agtgtcacgc caagatcggc caggtcgctg
     9721 gcttccatgc tggtcaacac tgtcacagca atcaaaagcg gtgcatcttt gccaaacgga
     9781 accagtgcct cacgcgctgc ggtcatcata cgcgccccac cagaggcatg aacattcacc
     9841 atccacacgc ctaagtcagc tgcagcagcg acagcgtgcg ctgcagtgtt ggggatatcg
     9901 tggaatttca ggtcaagaaa gatatcaaaa ccacgctgtt gaagttcgcg cacaaactgt
     9961 ggcccaaaca atgtaaacat ctctttgccg accttcagac gacaatcgcg tgggtcgatc
    10021 ttgtcgacaa aggccagcgc ggcatcacga ttatgataat caagggcaac aaccacagga
    10081 gaattcgtaa cagcgcggga agaagatgaa gcagttaacg tcatgaccag accttcttga
    10141 tgatgggcac cggaagtggc gcgaaacagg taaacggcga gcattctacc cgtggaaata
    10201 aaaaattaac agttgcgatt tctcccctgg caggcttttt ctgctctgtc gttgccggag
    10261 cacaatggaa catcaataac tagtatgttg taactaaagt cggttttttt aaaaaattac
    10321 aggccatcaa gaccgcgaat cggtttaatg gttgaccagg cacgacaaga cggacaatgc
    10381 cagtagaggg tgtatgcggt aaaaccacat ttctggcagc gataacgagg cttactacgt
    10441 accttctcgc caaccatgtc acgcagcacc atcaggctct ctttggcacg cccttcttcc
    10501 gcttcattta agtggtaatc cattaactta tggaacacac gcatggtcgg atgacgctga
    10561 agctggcgcg taatatagac ctgtgcggcc tcactaccgt cgcgcgcttc gatgatatcc
    10621 gcaagcatca attcagcatc ggcgccggtg ttctcttcca ccgcgcgctg caggaattct
    10681 gcccattcgg cagttttacc caactgctga tagcaggttt gcaacatttc cagcgtttcg
    10741 ctgaccagtt ctctgtcctg ggatatgaca cgttgcagac tttcgacggc tttggcgtat
    10801 tctcctttcg ccataaacac gcgtcccatc atgatggata cgcgggcgct gtttttatct
    10861 gccgccgccc ctttttttag caaggtcatg gcacgatcga gatcgtcgct ggccatatgc
    10921 tgcagggcta actcacagta gaaatgggca atttcgacgc gctgtttatc tttacccagc
    10981 ttcaccaggc gttcggcaac atcaattgct ttctgccact cactggtagc ctggtagatt
    11041 tgtagcaact gttgcagtgc gccaatgcgg aagtcagttt catcggtcag ctgattgaac
    11101 atgtcttccg cgcggtcata taacccggcg gccatgtaat cacgccccag ttgttgaatc
    11161 gccaacagac gctgttcata ggtcagcgag gcgctttcca ttagggtctg atggatgcga
    11221 atagcgcgat caacttcgcc acgcgaacgg aacaggtttc cgagcgtaag gtgggcttca
    11281 acggtgcctg tatcctcttt aagcatatcg agaaacaggt ctaccgcttt atcctgttga
    11341 ttactaagca ggaagttaac ccccgctacg taatcacgcg acaagcggtt ggcttcatct
    11401 tgcttgtttt gttgcgcact tctgcggccc atataccagc cataggcagc ggctacaggc
    11461 aaaagcagaa acaacaactc cagcatagaa agttattcct tcgccgctga cgagtgctgc
    11521 actacagcca cgtcagtcgc gggtgaaagc tggttttcca gtcgctttat tttacgttca
    11581 gcgcgcgcca gggaaacacg aactcgcagc cagaacaggc cacaaatcaa ccaaccgata
    11641 gcaaaccccg cagcaaacaa taccgccagc aatgtggaaa tacggtactc cccttgcgct
    11701 aacagataat taaacgtcac ctgttgatcg ttctgcgcac ccaacgtgac cgaaatcacg
    11761 aagatcgcta acaccagtaa gaaaatgagt aaatatttca cattacttcc cgttatgtgg
    11821 ttagagcgaa taaattatgg tcaacttacc gcaatagccc tttaaagtac cattttcgcg
    11881 ccgtctgcga aatggcatcc acgcgcattg ttgatgattt aagggctaaa ttcggaaaat
    11941 caaccagcgt taactttctt gttctcgttg cgctatttcg cgattttctt ccgcaggtgg
    12001 tgttaatggc ccacaaattc gttgcgcaag ccacgttgcc accgccacca gtgcccaaga
    12061 aatcaacgta gctaccacca gatcgcgtgg ccaatgcatc ccgagcagca ggcggcttcc
    12121 catgactccc gttgcccaga ccagcaagat agcaatggtt aatgttcgcc gacgcggcca
    12181 cagcaaacca acggccagca gtgcccaact ggcagcaaac atcgtgtgac cggaaggaaa
    12241 ggcaaacccc gtctctttct gccagtgtga acgcaaatat tgtggaatat ttttctcttc
    12301 agccaactgt tctttcacta aatttccgcg ttctgctcgc tttaaagtgt agaactcatc
    12361 aaccggaata tgatgtgttt tttccagcca gataacaaaa ggtcgtggtt cctggacttt
    12421 gtctttgatc caggatttaa cgccttggcc cacaaggatt gcggccgcca gaatggcaaa
    12481 taatacaaag gcagccttaa tgcgaaaacg cagacaccag agaaaccagc cgaataaaat
    12541 caaatgtgta atgacgcccc agggctgggt gacagtttca gtaacccaaa aagccgcttt
    12601 tagtagccaa ctttgttctc caggttgcca acgccagcca gaaatccata cggctactgg
    12661 cataacaagc aatagtgcag ctcccactgc ggtacgtctg gcaatcgaac gcatggcctc
    12721 tcctttttga taagtcccac aatcataact gaaaatgcca gttccaggaa aaattgacag
    12781 atttgtgcca ttccgtgaac gatcgacgcg tcgtgattag gtgaacccct tctcgttatg
    12841 gcaaaataag ccaatacaga accagcatta tctggagaat ttcatgcagc ttaaacgtgt
    12901 ggcagaagcc aaactgccaa ccccatgggg cgatttcctg atggtgggat ttgaagaact
    12961 ggcaaccgga cacgatcatg tcgcgctagt ctatggcgat atttccgggc ataccccggt
    13021 acttgcgcgc gtccattccg aatgtctgac cggtgacgcc ctgttcagct tgcgctgcga
    13081 ttgtggcttc cagctcgaag cggcattgac gcaaattgcc gaggaaggcc gtggtatttt
    13141 gctgtatcac cgtcaggaag gtcgtaacat tggtctgctg aataaaatcc gcgcttacgc
    13201 actgcaggat caaggttacg atactgtaga ggctaaccac cagttaggct tcgccgctga
    13261 tgagcgcgac ttcactcttt gcgctgatat gttcaaactc cttggcgtca atgaagtccg
    13321 cttgttaacc aataacccga aaaaagtcga aattctgacc gaagcaggga ttaatattgt
    13381 tgaacgcgta ccattgattg taggtcggaa ccccaataac gaacattatc tcgataccaa
    13441 agccgagaaa atgggccatt tgctgaacaa ataaccctct tgcattgtgt aattaatttg
    13501 cttgccggaa gcaaaataac cggcaagcaa atacttgtta cttcaacata ttacgaatga
    13561 cataatgcaa aatgccgtcg ttctggtagt aggtcaactc cgtcgcggtg tcgatacgac
    13621 aacggcaggg tacgacttcc tggctaccat ccgcgcgcgt aagcgtcacc ggaaccgtcg
    13681 cgccgggttg tagattttgc agatcgccaa tatcaatctt ctcttccccg gttagcccta
    13741 acgttttacg cgttacgcct tgcggaaatt ccagcggcag gatgcccatg ccaattaaat
    13801 tcgaacggtg aattcgttca aacgattcgg caatcaccac acgaatacca agcagacgcg
    13861 gaccttttgc cgcccagtca cgactggagc ctgatccata ctctttcccg gcaatcaccg
    13921 ccagcggcgt ttgctcctgc ttatagcgca tcgcggcatc atagatagag acaacatcgc
    13981 tgtcaggtaa atgccgcgtc atcccccctt caacgccagg caccatttca ttacggatgc
    14041 gaatattggc gaaggtgccg cgcatcatca cttcatggtt accacgccgc gaaccgtagg
    14101 agttaaagtc tttccgctca acaccccgac cttgtagata tcgacccgcc gggctgtcgg
    14161 gcttaatact gcccgccgga gagatatggt cagtggtgac tgaatccccc agcattgcga
    14221 ggatccgcgc accgtgaata tcttccacag gtgctggtgt tgcctgcatt tcatcaaaga
    14281 aaggcgataa gcgaatatag gttgaatcct cctgccaacc gtaggtatcg gatcgtgtga
    14341 cgttaatttc cttccactct gctgtgcctt caaaaacttc tgcgtactct ttacggaaca
    14401 tttctgtgga gacttgttct accgcacggg caatttcttg tgccgatggc cagatatctt
    14461 tcagataaac cgggtcgcct ttacgatcat gaccaatagg ctcagaagcc aggttgatat
    14521 tcatatttcc cgccagcgca taggcaacca ccagcggcgg cgaggccagc cagttagttt
    14581 taaccagcgg atggatacgg ccttcaaagt tacggttgcc ggacagcact gcaccgacgg
    14641 ttaaatcgcc ttttttgatt gccgtttcga taggatcggg cagcggccca gagttaccaa
    14701 tacaggtggt acaaccgtat cccacaaggt taaaccccag ttcgtcgaga tacggtgtca
    14761 atttcgcttt tgccagatag tcagaaacaa ctttcgagcc tggtgccagc gacgctttga
    14821 cccacggttg ccgcttgagg ccaagagtta cggctttttt cgccagcaag cctgcggcca
    14881 tcagcacact tgggttagag gtgttggtgc acgaggttat cgcagcaatg accacagcgc
    14941 catcaggtaa ctgatactga tgtccgttca taacataatc gaccggctgg cgatctttat
    15001 gcgtggcatt cacttccagt tcgttactgg cggcaaatgc ttttggtaca tcgggcagtg
    15061 caacgcgatc ctgtgggcgt ttaggccctg ccaggctcgc ttcaacgtca ttcatatcca
    15121 gttctaacgt actggtaaaa atcggttcat cgcccgggtt acgccacatg ccctgcgctt
    15181 tggcatattt ttcgaccaac tcgacctgat cttcgctgcg cccgcttaaa cgcatgtaat
    15241 cgagggttac agcatcgatt gggaagaagc cacaggtggc accatattct ggcgacatat
    15301 tggcaatggt ggcgcgatcc gccaacggta gtgaatccag accatcacca taaaattcga
    15361 cgaatttccc cacaacgcca tgtttgcgca gcatttgggt aacagtgaga accaggtctg
    15421 tggcggtaat accttcacgt aattttccgg taagtttaaa gcccactacg tccgggataa
    15481 gcatggaaac cggctggcct aacattgcgg cttctgcttc gatgccaccc acgccccacc
    15541 ccagcacgcc aaggccgttg atcatggtgg tgtgcgagtc agtaccaacg agtgtatccg
    15601 gataagcaat ccattcaccg tcctgcaatt cgctccacac tgctttgccg agatattcga
    15661 ggttaacctg atggcaaatg cctgtgcctg gcggcacgac gctaaaccga ctgaacgctt
    15721 gctttcccca tttcaggaac acataacgtt cgtggttgcg ctccatttcc aggcgtacgt
    15781 tttcttcaaa tgcctcatca tcaccaaaac gatcgacggt caccgagtgg tcaatgacca
    15841 ggtcgaccgg tgagagcggg ttaacctttg cagtatcgcc gccgaggcgt ttaaccgctt
    15901 cgcgcattgc agcgagatca acaacggcag gtacgccggt aaagtcctgc atcagcaccc
    15961 ttgccgggcg gtaggcaatt tcacggtcag catgggcatt tttcagccat cccgccagcg
    16021 cgtggatatc ctcttcggta accgagttac catcctgcca gcgcagcagg ttttcgagca
    16081 aaactttgag tgacttgggt agacgggtga tatcgcccag tgatttggca gcaagcggca
    16141 ggctgtagta gtggtaagtt ttatctttgg cctgcaacgt gtccttactg gcttctcgta
    16201 gggttgacga catagctcct ccttaatgac agggttgcga agccctgatt ctcttcaggg
    16261 tgaatattaa agataacaca aacatcgcgt aacgatttga taacaaccca aattgataaa
    16321 agaggagaaa gcttgtggca aagcaaatga aaaccccgcc gcagcggggt cagggatcag
    16381 tgagtaatga tccagaggag ttcggcccag aagagcatcg acagagaaaa cgcgcccatc
    16441 caggaccaat atttcatatt tgtgttgatc ataggctaca cccttaacgg ttattacttt
    16501 gtgttgagat aactcaacct gttattgctg cggtgtatta aaagcccttt aattcattaa
    16561 aaatgggtaa taagaatgat gataattatt gtttcggctg atcatcttca gcaaacagtt
    16621 ctataaaggc ttgctgctgc gatgttagtt gccagtcgtc tggaataacg acggaacatt
    16681 cccgtttcgt ttctggttca ttgtcactat cctggcataa tggtgacgac tccagttgct
    16741 cctgaccaac catagttatc cccacacttt ccaaatcagc agtgcgacaa ccacccagaa
    16801 aagtgcagaa ccaagaaaaa ccgccagcca ggctttacgt tttaattcag gatcgcggcg
    16861 gggttcctga tttccagaag gcattgctaa cctcatataa tcgccatcac ttatcagcaa
    16921 gacgttgaac gattggtgta attaatcata cgatgagaca aatcctaaag aaactttagt
    16981 caaaaatcca gcgaatttac gaatcgtctc cagtgaataa ttcaatcttt tgacctgaaa
    17041 tagatatttg aaaactggaa tttgcgaggc gggtaattag acactacttt ataggtaatt
    17101 taccctataa atatg
//
LOCUS       DAZUGK010000096        16879 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZUGK010000096 DAZUGK010000000
VERSION     DAZUGK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.096"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(164..979)
                     /gene="djlA"
                     /locus_tag="ACHY5W_002716"
     CDS             complement(164..979)
                     /gene="djlA"
                     /locus_tag="ACHY5W_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFS4447592.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1234..3588
                     /gene="lptD"
                     /locus_tag="ACHY5W_002717"
     CDS             1234..3588
                     /gene="lptD"
                     /locus_tag="ACHY5W_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFS4447593.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGLLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3641..4927
                     /gene="surA"
                     /locus_tag="ACHY5W_002718"
     CDS             3641..4927
                     /gene="surA"
                     /locus_tag="ACHY5W_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFS4447594.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            4927..5916
                     /gene="pdxA"
                     /locus_tag="ACHY5W_002719"
     CDS             4927..5916
                     /gene="pdxA"
                     /locus_tag="ACHY5W_002719"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFS4447595.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSRAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRTQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            5913..6734
                     /gene="rsmA"
                     /locus_tag="ACHY5W_002720"
     CDS             5913..6734
                     /gene="rsmA"
                     /locus_tag="ACHY5W_002720"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4447596.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            6737..7114
                     /gene="apaG"
                     /locus_tag="ACHY5W_002721"
     CDS             6737..7114
                     /gene="apaG"
                     /locus_tag="ACHY5W_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFS4447597.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7121..7969
                     /gene="apaH"
                     /locus_tag="ACHY5W_002722"
     CDS             7121..7969
                     /gene="apaH"
                     /locus_tag="ACHY5W_002722"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFS4447598.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLVAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(7966..8280)
                     /locus_tag="ACHY5W_002723"
     CDS             complement(7966..8280)
                     /locus_tag="ACHY5W_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFS4447599.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFI
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8283..8516)
                     /locus_tag="ACHY5W_002724"
     CDS             complement(8283..8516)
                     /locus_tag="ACHY5W_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFS4447600.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(8602..9081)
                     /gene="folA"
                     /locus_tag="ACHY5W_002725"
     CDS             complement(8602..9081)
                     /gene="folA"
                     /locus_tag="ACHY5W_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFS4447601.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVKWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9273..11135)
                     /gene="kefC"
                     /locus_tag="ACHY5W_002726"
     CDS             complement(9273..11135)
                     /gene="kefC"
                     /locus_tag="ACHY5W_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFS4447602.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNKRNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11128..11658)
                     /gene="kefF"
                     /locus_tag="ACHY5W_002727"
     CDS             complement(11128..11658)
                     /gene="kefF"
                     /locus_tag="ACHY5W_002727"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFS4447603.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11769..13100)
                     /gene="yaaU"
                     /locus_tag="ACHY5W_002728"
     CDS             complement(11769..13100)
                     /gene="yaaU"
                     /locus_tag="ACHY5W_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4447604.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQLQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13158..13445)
                     /gene="fixX"
                     /locus_tag="ACHY5W_002729"
     CDS             complement(13158..13445)
                     /gene="fixX"
                     /locus_tag="ACHY5W_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFS4447605.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKIDADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13442..14728)
                     /gene="fixC"
                     /locus_tag="ACHY5W_002730"
     CDS             complement(13442..14728)
                     /gene="fixC"
                     /locus_tag="ACHY5W_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4447606.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14779..15720)
                     /gene="fixB"
                     /locus_tag="ACHY5W_002731"
     CDS             complement(14779..15720)
                     /gene="fixB"
                     /locus_tag="ACHY5W_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS4447607.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAKA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGTDGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15735..16505)
                     /gene="fixA"
                     /locus_tag="ACHY5W_002732"
     CDS             complement(15735..16505)
                     /gene="fixA"
                     /locus_tag="ACHY5W_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFS4447608.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4169 a         4682 c         4211 g         3817 t
ORIGIN      
        1 tctcaacctt aagcagggtc aacgcgataa aaccaacaga aaccctcccg taaaccgtgt
       61 attgagcctg tccaccactc tgagtaaacg ccttttctca aagaagtgac cgccagggcg
      121 gccaccttta atcgtgggca gttactcagc atcatttaca gggtcactta aaccctttct
      181 gctgcttaat caattcatag gcctgctgaa tttcctgcgc tttttgcttc gccatctcca
      241 tcatctcagg cggcaaacct ttcgccacca acttatcggg atggtgttca ctcatcagct
      301 tacggtaggc acgtttgatg gtggtcgcat catccgtcgg cttcacgccc aaaacattac
      361 aggcatcttc cagcgtcggg ccacgctgcg cttgctgcca gttaccacca ccagattgct
      421 gctgataacc gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact
      481 gggcgcggga gatccctaat tcttcagcaa tgacatacag caccgcccgt tcattcgggt
      541 gcagtgaacc atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt
      601 caaaacgacc aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt
      661 ctgattttcc cacccggaac gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca
      721 ttcggtccat caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt
      781 tggttaaatg ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct
      841 ggttggcgaa ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca
      901 gacctaacac tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga
      961 tttttcccca atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc
     1021 acaatataaa ggtgctttta ccgttttctg gctgcggtca attgtgggac atcgcctata
     1081 atttgcatta tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac
     1141 aggattaaca ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca
     1201 acgttaccga tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg
     1261 gccaccatga ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca
     1321 cagtgcatgt tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac
     1381 ttacccgtga ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt
     1441 actggcagcg tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc
     1501 catcaaaaag aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat
     1561 gtccattacg acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac
     1621 accaaagata ccaacgtctg ggagggtgat taccagatgg tgggtcgcca gggccgcggt
     1681 aaagcggacc tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc
     1741 tttacctcct gtctgcctgg ttctgacacc tggagtgtgg taggtagcga aattattcat
     1801 gaccgcgaag aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg
     1861 atcttttata gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg
     1921 atcccgaacg ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg
     1981 aacatcgcgc caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac
     2041 atcatgtggg agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg
     2101 gactatctgc cttcagataa agtctatgaa gatgaacacc cgaacgatga tagttcacgt
     2161 cgttggttgt tctactggca acactccggg gtcatggatc aggtgtggcg tttcaacgtc
     2221 gactatacca aggtcagcga tcctagctac ttcaatgatt ttgataacaa gtacggttcc
     2281 agtactgacg gctacgcaac gcaaaaattc agcgttggct atgcagtgca aaacttcaat
     2341 gccaccgttt caaccaagca gttccaggtc ttcagcgagc agaacaccag tagctactcg
     2401 gcagagccgc agttagacgt taactactac cagaatgatg ttggtccgtt tgatacgcgt
     2461 atttacggcc aggcagtgca ctttgttaac accagagacg acatgcctga agcaactcgt
     2521 gttcacctgg aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc
     2581 gaagcgaagt tgctggcaac ccattatcag caaaccaatc ttgactggta taactccaga
     2641 aacacaacca agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc
     2701 aaaatggtct ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa
     2761 ccgcgcgcgc agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac
     2821 tcatctctgc tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt
     2881 gaccgtatag cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat
     2941 gctgccgttg aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc
     3001 actggcgatg acaacataac atgggagaat gacgacaaaa cgggcttact ggtgtgggca
     3061 ggcgatactt actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat
     3121 acacgtctgg ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac
     3181 cgtctggtac agctgaatta ccgttacgcc agcccggaat atattcaggc tacgctgcct
     3241 aagtactatt ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc
     3301 agctggccaa ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg
     3361 aacaagcaag ctgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc
     3421 gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac
     3481 aacgcaattg gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg
     3541 caagagatgc tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta
     3601 ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct
     3661 gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa
     3721 agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca
     3781 gtcggtaaaa ctgaacgctg ctcaggcaag gcagcagctt cctgatgacg cgacgctgcg
     3841 ccaccaaatc atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat
     3901 gggagtgaaa atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa
     3961 caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac
     4021 ctatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg
     4081 tcgtcgcatc accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca
     4141 aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc
     4201 gacctctgat caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg
     4261 taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa
     4321 cggcggccag atgggctggg gccgtattca ggagttgccg gggatcttcg cccaggcatt
     4381 aagcaccgcg aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat
     4441 tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc
     4501 tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc gcgtgaaact
     4561 ggaacagatt gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga
     4621 gttctcgcag gatccaggtt ctgctaacca gggcggcgat ctcggctggg ctacaccaga
     4681 tattttcgat ccggctttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc
     4741 accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga
     4801 taaaaccgac gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc
     4861 ggaagaagca gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag
     4921 caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc
     4981 ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc
     5041 gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgacct
     5101 tattccccca actcccctgc acaaccgcaa actgcgggca cattaacgct acttcctgtc
     5161 gcgctacgtg cacctgtcac tgcggggcag ttagcggttg aaaatgggca ttatgtggtg
     5221 gaaacgctgg cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca
     5281 ggccccgtgc ataaaggcgt cattaacgac gcaggcattc cgtttaccgg tcataccgag
     5341 tttttcgaag agcgttcgcg ggcgaaaaag gtggtgatga tgctggcgac cgaagaactt
     5401 cgcgtggcgc tggcaacgac gcatttacct ctgcgcgata tcgcagacgc tatcacccct
     5461 gcacttttgc acgaagtgat tgctattttg catcacgatt tgcggaccaa atttggtatt
     5521 gccgaaccgc gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg
     5581 ggcacggaag agatagacac catcattccc gttctcgacg aactgcggac gcaggggatg
     5641 aaactcaacg ggccgttgcc ggcagatacc ctgtttcagc cgaaatatct cgacaacgcc
     5701 gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc
     5761 gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc
     5821 accgcgcttg aactggcggg acgtggcaaa gccgatgtcg gcagttttat tacggcgctt
     5881 aatctcgcca tcaaaatgat tgttaatacc caatgaataa tcgagtccac cagggccact
     5941 tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg
     6001 tttctgccat taacccgcag aagggtcagg cgatggtcga aatcggcccc ggtctggctg
     6061 cattgaccga accggtcggc gaacgtctgg accaactgac ggttatcgaa cttgaccgcg
     6121 atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc
     6181 aggatgcgat gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg
     6241 ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata
     6301 ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg
     6361 caggaccgaa cagcaaggcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg
     6421 tgatcccagt gctggaagta cctccgtcag cctttacacc accacccaaa gtggattccg
     6481 ccgtcgtgcg cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgcgtgt
     6541 tgagccgcat caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg
     6601 gcaacctgtt tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg
     6661 aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt
     6721 tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta
     6781 cattgaggca caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat
     6841 acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg
     6901 caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc
     6961 gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat
     7021 gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt
     7081 attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg
     7141 cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc
     7201 tgggaaagat accctctggc tgacgggcga tctggtcgcg cgcggcccgg gttcgctgga
     7261 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga
     7321 tctgcatctg gtggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac
     7381 accgctgctg gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccaacctct
     7441 gctgcaaatc gacgaagaga aaaagctggt gatggcccac gcagggatca cgccgcagtg
     7501 ggatctgcag accgccaaag agtgcgcacg cgatgtagaa gcggtgctgt cgagtgactc
     7561 ctatcccttc tttctcgacg ctatgtacgg cgatatgcca aataactggt caccggaatt
     7621 gcgggggctg ggaagactgc ggtttatcac caacgccttt acccgtatgc gtttttgctt
     7681 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaagccc ctgccccact
     7741 gaaaccgtgg tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca
     7801 ctgggcatcg ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg atactggctg
     7861 ctgctggggt ggtacattaa cctgtctgcg ctgggaagat aaacagtatt ttgtccagcc
     7921 gtcgaaccgg cataaagata tgggtgaggg agaggcggcc gcgtcttaaa tcccgtcgag
     7981 cataaaatct aatgctcctt taatcaacgt tcggtgcgcg ctggcatcgc aaaatttcgt
     8041 tcccagcgcg ttaactggaa cagttgcagt ctcatgcgtc atgagcacat attctttgcc
     8101 gtctaccagt aaaaggattg ggttaagccg ttctggtggg cgaatacgga taaatcgttc
     8161 aataggtgtt aatggaataa caatacggcg attaatcaca ccaataatgt cgctggtaac
     8221 atcgataaca aagggaaacg cggcgttcct gctgcgactg cggtataccg taaattgcat
     8281 cgctagaagc tccggtactc atcagagaaa catccggttt catcagccaa ttgatttaac
     8341 gcagcgatag cttctgcgtt ctcttcacgc caacgtgttg ctgcatgttt tttaagttca
     8401 gcattgagcg caaccgtaag ggtggcgctc atatttaagc ctgcatcacg agcgcgattg
     8461 actaactcac ggtcgacggt gacggtcaca ctttgtgtgg tacgttttgc agtcatgata
     8521 tcgtgctcct gtacaattta tacgtctagt atacacggat atctgcacgt atcagtgatg
     8581 ccggaattct aatatacaaa attaccgccg ctccagaatc tcaaagcaat agctgtgaga
     8641 gttctgcgca tcggcgtcgt ggaattcgct gaataccgat tcccagtcat ctggctcgta
     8701 atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat acagtttttg
     8761 cgcttttgcc aggaactgct cataaacgcg accgccgcca atcaccatga tttctggtac
     8821 gtcaccacac gccgcgatgg cttcatccac cgacttcacc cactttacgc gatcgtccgt
     8881 acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac cgattgattc
     8941 ccaggtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa accaggcgag
     9001 atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat ctaccgctaa
     9061 cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc cactatacgt
     9121 aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt tatattctgc
     9181 tggcgagtcc acgctctccc cctggactcg ccgcattaca atgaaacaaa aacaaacagt
     9241 tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt ttccggttca
     9301 tccgccatgt tgccggtatg tttaccttct tctgttccct gccagccatg tcgttgaatt
     9361 aatgacagat gttcgcggtc ctcggtgatg atctcactta acatcgcgct ggtgcgttta
     9421 tagaccgccg cgcgggcttt ggtgtcattc tcaaccattg ccatctcttc caccatctga
     9481 atattaaagc ggcggaacac atcggcacgt tctcgcgctt catacggccc cagacctaaa
     9541 ctttccagcg ccagacgccc ggttttcagc gcaccttcaa aggtttcacg ctccggcttt
     9601 tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg ggcaataatc
     9661 tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt ttgcggatcg
     9721 tcaatagcat taatcagcac ttccgctttc gccgctccgg cagattccag taaatccatc
     9781 cgcgtggcat cgccgtaaaa cactttcata ccaaatttac gcagcgtttc gatatggtcc
     9841 ggatcgtgat cgagcaccac cattttcacc ccgctggaga gcagtaaacg tccggtaatc
     9901 tgtccaaaac gaccgaatcc ggcgataatc acgcgcggct gttcttcatc gatctcatcg
     9961 gcttcacgcg cttcctcagt agaagattgc tcaaggcgat tgaggatcac cagcagaatc
    10021 ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg atttcgccca ctccggctcc
    10081 agcacattcg ccatctgcgc cgcaccaaac accacaaagg caaactcact accctgccct
    10141 aacaacaccg caaaccagcg acgctgttta tttggcacct gcaacggtcg ggcaatcagc
    10201 catagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg
    10261 ttttcaagca gcgtgccaaa atctatcgac atgccaacac cgatgaaaaa cagccccagc
    10321 agcaaacctt taaacggttc gatatcgctt tccagcgcat gacggtattc cgagcttgcc
    10381 agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc ttccagcagc
    10441 aaaccaaagc caaacacaag gaataacgcc acggcactaa acacttcccg caagccagag
    10501 cgggcaacaa aacgcagcgc cggacgcgta acatagcgcc ccagcaatac caccagtacc
    10561 agcgcccccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg
    10621 ctcgcagcca gtagcggaat catcgccacc aacgggatcg ccgcgatatc ctggaacagc
    10681 agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttt attcatcgcc
    10741 tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc cgcgacctgc
    10801 cagcgcagcc caagtaacat acagaacagc cccagcaggc cgccgcaaat caccatctgc
    10861 aatgcgccac cgccgaacac tgccgcacgc agcttccaca gcctttgtgg atcgagttcg
    10921 aggccgataa taaacagcat cagtaccacg ccaatctcgg caaagtgcag aatagattcg
    10981 gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat caggtagcca
    11041 agtaccgatc ccagaccaag acgtaccgca atgggtacaa tcagcgccgc cgatccgagg
    11101 tagatcagcg cctgaatcag cgtatggcta tccatgatgt gcctcctgcc attccagcag
    11161 acgttgctta tagtggcgcg cctgcccttc gagggtttcg tcgtcacaaa taaaggtgca
    11221 gtgcatggca aacggaggca gccagttcag cccgcagtag attgccgtcg cctgtagcgg
    11281 ctgcgacagc acatcaaagc ccggatgcgc accaatttca aaatggcttt ccccgccgcc
    11341 ggtcgtcacc gcccacagca aatgtttgcc atgcagcgcc gtgccgccgt gaccgtaagc
    11401 ccagccgtgc gagaaaactt tatcgatcca aagtttaaga agcggaggaa tgctgtacca
    11461 ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct gctcggcggc
    11521 aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga cgccttccag
    11581 cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat acggatgcgc
    11641 ataaattatg aggatcatag ttattagccc gcgtgtttac gcctttgttt tcccacaaag
    11701 tgtagtcata tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc
    11761 tgaacaaatt atcccattct ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac
    11821 attcctcgag tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc
    11881 gcccccatta gcatcgtgtt actgataccg taattattga taaagatcgg tagtgcccag
    11941 gtcgaaacaa tggtgccaat acggcttaag gacataatca cgcccacggc agaggcgcgg
    12001 atatctgtcg ggaagagttc attaggatag agccactgca aattacccgg cccgccagag
    12061 aaaaaggcat ataccgcaaa ggccatcact accagccaga tccccatatc cgggattagc
    12121 cccaaaaccg ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt
    12181 ccggcagtgt ttaaccacag catcggcgga atacagccga gcataaagaa caggctaatc
    12241 accacgttcc ccagcgccgc gtttttgcca acccccaatc ccaacaaacc aacgatttgc
    12301 ggaccaaagg tataaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca
    12361 aacagaacaa aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcagc
    12421 tgctcttcat cgaaagccac cggttcgcca aacagtttta tcatcatctc ttcgcactct
    12481 tttactcgcc ctttgcgtaa taaccagcgg ggagattcag gcagttcgaa tcgaccaatc
    12541 aaaatcaaca aacaggggat cgctgcgcta cccagcatcc agcgccagcc gccttccaca
    12601 tcataaagcc agtagccgac cagatcggca caggtcgcgc cgacatacca catggcggcg
    12661 ataaagctga tggaaaacgc ccgctgacgg gtactggaga actcggtgat cattgaggtg
    12721 gcgatgggat aatctgcacc gatgacaatg ccgatgagta cccgcatcac caacagttcg
    12781 acgggggatg aaacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg
    12841 aggaacattt tgcgccgtcc gactttatcg gagatatagc caaacagcga tgtgccaacg
    12901 aacagcccgg cgagcgttcc cgcgcccagc aagccaatcc agtcagcgtc cagtttcagc
    12961 gccggagtca gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac
    13021 ggaccaccgc ttccccacag caaaatgcgg cggtgaatag aggagaattt gagatcgtca
    13081 aagtttctgg acggttgcat gggttattcc tgttttttgc aatgagagtc agtgcggcgt
    13141 tacgtatcaa accaacatca gccgtaacgg aactccacac caaaggtgcc gcgcgggtat
    13201 tcccactgtt ccagcgccga ccccagcccc agaatgcgac aggtgccgca ctccagacat
    13261 ccggcgtaat cgaagcgcac actgccgtca tcctgcttct tgtacagacc tgcggggcac
    13321 gctttcacca gcagctccag ggcctgttta tcggcatcga tcttcacaac aatgtgcgga
    13381 tgctcttcat cgacattgaa tttattgacg cccagtttga cgtccacatt gacgggagaa
    13441 gtcataaaac ggtcactcct ttcatgccat ccttgatcag attgatgaag cccactttct
    13501 tgccgtggcg gaggattttc ttgcgcatca gttccggcgc actgccgtca atggtgaaca
    13561 ggtcacgcgc cacgcctacc gccagttccg ggtagccgct aaacatgcgt gggttatcaa
    13621 ggaaggccgg tagtttctgg tacatgcgca tatcgcgcag cgggccactc tcaagatgct
    13681 gacgatattc cgcgagtttt tgcttactga aatcgtcgct tttcatcgct gaaagcacgg
    13741 tttttgctgc ggcttccccg gcggcaatcg ccagatccat accgcgaatg gtaaaaccga
    13801 ggttcataca cataccggcg gcatcaccgg caatcaatac gccgtcacca accaactccg
    13861 gcagcatgtt gatgcctgct tccggcacta cgtgagcgga atattccacc agcttgccac
    13921 ccgcgatcag cggtgcaacg gccggatgct gtttgaaatc ttccagcatt tgcggcaccg
    13981 atttttttgc gtcatgcaga tgatgcagac cacaaaccag ccccagcgac agggtgtttt
    14041 cattggtata aaggaagccg ccgcccatca ggccatcggt gggtgatccc gcaaacaggc
    14101 aagccgcccc ctgattaccc tgcaactgaa aacggtcttc gataaccgac ttcggtaact
    14161 cgatcagttc cttcacgcca accgccacat ccgtcggttt aacgcgtttt gccatcccca
    14221 gcttttcggc aaggatggag ttcaccccgt cagcaaggat caccgttttc gcttcaatca
    14281 catcgccatc ggcttctacg ccgacaactt tgccatcgcg ctgtacgagg ttatcgacgc
    14341 ggatcccggt aattaactgc gcgcccgctt cttcggcctg ctccatcaac caggcatcaa
    14401 atttactgcg caaaacggag taagaacgct gggatggcga ggtttcgtca ccgttgcagt
    14461 agtccatagt catcgctgac ttttccgtca taaacgcgag tttttcatgg gtgatcaggc
    14521 gttctacggg ggcggattcg gcgaaacctg gaataatgtg ttccagactg tgggcataga
    14581 gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc acgctcgata actaacactt
    14641 gcgcaccttc gcgggcgagc accagtgcgg caaccgaacc ggcaagccct gcaccgacga
    14701 tgatggcgtc aaagatatct tcggacataa gagctccctg tcagcggcag gaccgtaatg
    14761 cagccctgcc agagtggatc aacgcgctaa agctgcggtc agcgccggaa ggatcttcac
    14821 ggcgtcgcca acaatgccgt aatccgcgta ctggaagatc ggcgcatttt tatctttgtt
    14881 gatggcgaaa atggtttgcg atgcattagc accaaccatg tgctggatct gcccggagat
    14941 ccccaccgct aggtacagtt caggtttcag catcaggttg gagataccga catagcgttc
    15001 gtgctccatc catttttcgt tttccgccac cggacgagaa caggccaact ccgcacctat
    15061 cgccttgcaa agctgctctg ccagcgcaat gttctctttg ctgccaatgc cgcgaccgac
    15121 gctgaccacc agacgggctt tgtcgagatc gacgctgttg ctctggcgcg cctgggtcgc
    15181 cgtgcgggta atcgccacag ccggagcctg ccactccacg gtatgcgttt cgccagtgcg
    15241 tgacgcgtct ggctgagccg catcgaacgt gccactgctg atggtcagta ccgcatacgg
    15301 cgtggcaatg cgttcttcgc caatcgccag gccgccgtaa accatgtgtt tcactgttgc
    15361 tttaccgtcc tgtacgctga cggtgctggc atcgttagac accgccgctt taaggcgata
    15421 acccagtttt gccgccagta atttgccgcg acgggtgttt ggcaacagca ccaggccgtc
    15481 tgtgccgtgc tggcgaatag tgtcagccat gacactggcg taatcttcga tcatccgatc
    15541 gtccggtttg ccgtttaatt tccagacatg attagcgccg agctggattg cctttgcgcc
    15601 gtcggcatca ttgaggacaa aggtgttgat ttgattagct aaagcctgcg caccgttcat
    15661 cagttccggc agacgagaag gggtatcgct gaatacccag acttgagaaa acgtgttcat
    15721 agcatcccct gtaattaaat gactttgcga agattttcag caaatgcggc gatctgttct
    15781 tcgccgtcac cttcaatcac gatgcgctga cgctcgcgct gtttcggcgc ggcaacctgt
    15841 tgttctgacc aggctgcctc tgcgttaaaa ccgatatccg ccgccgacca tacctggact
    15901 ggctttttcg ctgcgccgag aatggctttc atcgaaggaa tttgtgggga gttgatatca
    15961 gtggaaacag caacaaccgc aggcagcgga atgcttaagg tttcggtttc atcttccagt
    16021 tcgcgctcaa cggtgagggt atctgccgtc agggagataa ttttgctgac gccgttaact
    16081 gccggaatat tgaggatttc gcccaccagc agaccaacct gctgggcata aaggtcggaa
    16141 gaaccatcgc cacagaggat cagatcaaag cctgctttct gggcggctgc agccagtgcg
    16201 ctcgccgttt gttgcggcag tgcctgctcg aattggtcat caatcaccac aatcagttca
    16261 tccgggccgc gcgatagcac atctttacgc cctttggcgt tggtcagggc tttaccgccc
    16321 acacttaagg ctgtcacctg cgcctctgct gcctgttgct ttagctggca agccgcttca
    16381 atagcgttga gatcgtattg gcttattttg gcatcggctt tgctgaagtc taatgaacca
    16441 tcagcattat tgaccgcaat atcctgttca tcaggcacgc acttatagca agtaataatc
    16501 ttcattgcat ctccagaaat catgaaggaa acgtcgttca tatcggtggt gttaattaca
    16561 gatatcacgc cctttaattt ggaaataaaa ctttaatcac caatattgaa aatgtcacac
    16621 gcagataaat ccaactttca atattgttaa gttcctcacc aatattgaaa acatggcgta
    16681 gcaaaaagaa attttcaata ttgttttata gatcaccaat attgaaagtc agatgctatt
    16741 tttttaccca caaaagaaca taacaaactg attaataacc aaaattaacg gaaaaacgtc
    16801 ctctattcac ccgaaacaaa aatgtgacac caatcacaga atacagctta ttgaataccc
    16861 attatgagtt agccattaa
//
LOCUS       DAZUGK010000097        16863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZUGK010000097 DAZUGK010000000
VERSION     DAZUGK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.097"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..753
                     /gene="espX2"
                     /locus_tag="ACHY5W_002733"
     CDS             <1..753
                     /gene="espX2"
                     /locus_tag="ACHY5W_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFS4447609.1"
                     /translation="MRLSQQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPET
                     ICTRAFPLFAKGWEYAQRNKKHQLILNALGFKGYIRDVFMSAIMRKTDFVPECNNQPT
                     ELNSSFSSLMTDSDQWQQHSLKDKHYANLLTMLDLKEASESDKSKIFFCLSAVFANIS
                     HSNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEAN
                     QLRISSLYRDMVRYAQYRFSKVLSEWTPDAWV"
     gene            1036..3186
                     /gene="dinG"
                     /locus_tag="ACHY5W_002734"
     CDS             1036..3186
                     /gene="dinG"
                     /locus_tag="ACHY5W_002734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFS4447610.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYVLIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3214..4176
                     /gene="ybiB"
                     /locus_tag="ACHY5W_002735"
     CDS             3214..4176
                     /gene="ybiB"
                     /locus_tag="ACHY5W_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFS4447611.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIAQRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4317..5402
                     /gene="hcxB"
                     /locus_tag="ACHY5W_002736"
     CDS             4317..5402
                     /gene="hcxB"
                     /locus_tag="ACHY5W_002736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFS4447612.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5469..5528
                     /gene="ybiE"
                     /locus_tag="ACHY5W_002737"
     CDS             5469..5528
                     /gene="ybiE"
                     /locus_tag="ACHY5W_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFS4447613.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5631..5891)
                     /gene="ybiJ"
                     /locus_tag="ACHY5W_002738"
     CDS             complement(5631..5891)
                     /gene="ybiJ"
                     /locus_tag="ACHY5W_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFS4447614.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6157..6423)
                     /gene="ybiI"
                     /locus_tag="ACHY5W_002739"
     CDS             complement(6157..6423)
                     /gene="ybiI"
                     /locus_tag="ACHY5W_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFS4447615.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKQAYTGYNRRGSKDSQLR"
     gene            complement(6497..7174)
                     /gene="ybiX"
                     /locus_tag="ACHY5W_002740"
     CDS             complement(6497..7174)
                     /gene="ybiX"
                     /locus_tag="ACHY5W_002740"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFS4447616.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(7216..9498)
                     /gene="fiu"
                     /locus_tag="ACHY5W_002741"
     CDS             complement(7216..9498)
                     /gene="fiu"
                     /locus_tag="ACHY5W_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFS4447617.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPVGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATEDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9763..10023)
                     /gene="mcbA"
                     /locus_tag="ACHY5W_002742"
     CDS             complement(9763..10023)
                     /gene="mcbA"
                     /locus_tag="ACHY5W_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFS4447618.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10299..11225
                     /gene="rlmF"
                     /locus_tag="ACHY5W_002743"
     CDS             10299..11225
                     /gene="rlmF"
                     /locus_tag="ACHY5W_002743"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFS4447619.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11222..13447)
                     /gene="ybiO"
                     /locus_tag="ACHY5W_002744"
     CDS             complement(11222..13447)
                     /gene="ybiO"
                     /locus_tag="ACHY5W_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFS4447620.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(13564..14286)
                     /gene="glnQ"
                     /locus_tag="ACHY5W_002745"
     CDS             complement(13564..14286)
                     /gene="glnQ"
                     /locus_tag="ACHY5W_002745"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFS4447621.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14283..14942)
                     /gene="glnP"
                     /locus_tag="ACHY5W_002746"
     CDS             complement(14283..14942)
                     /gene="glnP"
                     /locus_tag="ACHY5W_002746"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFS4447622.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15080..15826)
                     /gene="glnH"
                     /locus_tag="ACHY5W_002747"
     CDS             complement(15080..15826)
                     /gene="glnH"
                     /locus_tag="ACHY5W_002747"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFS4447623.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16230..16733)
                     /gene="dps"
                     /locus_tag="ACHY5W_002748"
                     /gene_synonym="pexB"
     CDS             complement(16230..16733)
                     /gene="dps"
                     /locus_tag="ACHY5W_002748"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFS4447624.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         4125 a         4192 c         4459 g         4087 t
ORIGIN      
        1 atgcgcttat cgcaacaggc gttaatctca atgcttgagc cagaggttaa gaaaaagact
       61 atctggaata attactctat ttatccatct ttgcaggata cccatgaagt tgtcagagat
      121 gatccagaaa cgatttgtac gcgtgcattt cctttatttg caaaaggatg ggaatatgcg
      181 cagagaaata agaaacacca attgattctc aatgctcttg gatttaaagg ttatattcgt
      241 gacgtattta tgtcagcaat aatgcgaaaa acagactttg tgcccgaatg caacaatcaa
      301 ccgacagaac ttaattcatc attttcctct ttgatgaccg attcagacca atggcaacag
      361 cattctctga aagataaaca ttacgctaat ctattaacga tgctggatct caaagaggcc
      421 agtgaaagtg ataagtctaa aatatttttc tgcctgtctg ccgtatttgc aaatatttct
      481 cacagtaatg tctttaacgg tattcctgat gcgtcaaaaa ctttaaaacg ttatgcattt
      541 gctttattgg cgaaagctca ttctttagat gaaagtatga taagcaatca aacttttaat
      601 acatacaaaa cggtattgct tgattttaat aatctgtcta atgaagaggc caaccagttg
      661 cgcataagtt cgttatatcg tgatatggtc cgctacgcac aatatcgttt ttctaaagtt
      721 ttatccgagt ggacgccaga cgcatgggtg taaagatcgc agcagaataa tggcattgat
      781 ggccctggct caggccaggg cgggcaatac cgatttctgc gaagcgatga cggtggcaag
      841 tgtaaccacg aaatgcggta tcacttgctg ttttctgccc tgaagactgg cctcactgtg
      901 ccgtcgaact ttcaatcaaa tatattgcga gagggcgtgt ttgcgagccg ctttccagaa
      961 acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattgactg tttatacagt
     1021 atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat
     1081 aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt
     1141 gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat tgaagccccc
     1201 actggcgttg ggaaaacgct ctcctatttg attcccggca tcgccattgc ccgcgaagag
     1261 caaaaaacgc tggtggtgag caccgccaac gtggcattgc aggatcagat ctacagcaaa
     1321 gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc ttttgggcgt
     1381 gggcgctacg tttgcccgcg taatctgacg gcgcttgcca gcactgagcc cacgcaacag
     1441 gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga gcaaaaacgt
     1501 tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg cgatcatacg
     1561 gatatcgcta tagatgacga tctctggcgt cgtttaagta ccgacaaagc cagttgcctc
     1621 aaccgcaact gttactacta tcgcgaatgc ccgttttttg ttgctcgtcg ggagattcag
     1681 gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcg
     1741 gtattgcctg acccgaaaaa tttactgctg gtgctggacg aaggccatca cctgccagat
     1801 gtggcgcggg atgcgctgga gatgagtgcc gaaatcaccg cgccatggta tcggctacag
     1861 ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgacc gaagaccatc
     1921 ccgccgttgg cgatccctga acgtttgaat gcgcattgtg aagagctgta tgtgcttatc
     1981 gcctcattaa acaacattct caatctctac atgcctgccg ggcaggaagc agaacaccgt
     2041 tttgcgatgg gcgaactgcc tgatgaagtg ctggagatct gccagcggct ggcaaaactc
     2101 accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga gaaaaccggc
     2161 agccatgaca ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc gttggggatg
     2221 ttcgaggcgc aaagcaaact ctggcggctg gcttcgctgg cgcaatcttc cggtgcaccg
     2281 gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg gtttcactgc
     2341 gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt
     2401 attgtcacct ccgcaacctt gcgttcgctg aacagttttt cgcgtttgca ggagatgagc
     2461 gggctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc
     2521 gaacagggca aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagaa
     2581 cagcatattg ctgaaatggc ggcctttttc cgtgagcagg tggagagcaa aaaacatctc
     2641 ggtatgttgg tgctgtttgc cagcgggcgt gcgatgcagc gctttctcga ctatgtgacg
     2701 gatttacgtc tgatgttgct ggtgcagggc gatcagccgc gttaccgttt agttgaactg
     2761 caccgcaaac gcgtcgccaa cggtgagcgt agtgtgctgg tgggcttaca gtcatttgcc
     2821 gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa aatcgctttt
     2881 ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc
     2941 tatccatttg aggtgcaaag cctgccgagc gcctcgttta acctgattca gcaggttggg
     3001 cgactgattc gaagtcacgg ttgctggggc gaagtggtga tttacgataa acgcttgctg
     3061 accaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat agagcaaccg
     3121 gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg
     3181 cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag
     3241 atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat
     3301 gcccatatgc tcaatggtga agtccctgac cttgagttgg gcggtgtatt gattgcgcag
     3361 cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac
     3421 accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc cagttacaac
     3481 ggcgcgcgta aacaggccaa ccttacgccg ttgttggcga ttctcctgca caaactcggt
     3541 tttcctgtgg tggttcacgg ggttagcgaa gatccaactc gcgtgctgac cgaaaccatt
     3601 ttcgaactga tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac
     3661 gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg
     3721 gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg
     3781 ccatttgccg aaggtgaggc actgcgtctt tccagcgttt cgcatccgga atacatcggg
     3841 cgagtcgcga agttctttag cgatatcggc gggcgggcgt tactaatgca tggtactgaa
     3901 ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga ccgtgaaggg
     3961 atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttattgcc acaagcaaaa
     4021 gatccggaaa ccacggcgca gtggattgag cgttgcctgg ctggcagcga accgattccc
     4081 gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc
     4141 agcgatggcc tggcgcgagt taatcaggca ttttaattct tttctcagcc ggatgagcca
     4201 tgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt gaccttcccg
     4261 cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag ttaaccatgg
     4321 aaagtggtca tcgctttgat gctcagacgc tgcacagctt tattcaggct gtatttcgtc
     4381 agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc gcggcaaacc
     4441 tggcagggca tgattcacat ggtattggca tgatcccaag ctatgtgcgt tcctggagtc
     4501 aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg gcggcagtca
     4561 cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg gcactaggga
     4621 ttgagaaagc gcatcagcac ggtattgccg ccgtggcgct acataactcg catcatatcg
     4681 gccgtatcgg ttactgggca gaacagtgcg cagcggcggg gtttgtctct atccactttg
     4741 ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc cgctttggca
     4801 ccaatccgtt ctgcgtggtt ttcccacgta aagatgattt tccgctgttg ctcgattacg
     4861 ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc
     4921 cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg
     4981 agtcgccgtt gggggcgctg ttgacctttg ccgaacataa aggctacgcc cttgcagcga
     5041 tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag gaaacgttac
     5101 aaaccagtcc cgatgccatt cttaactgca tgaccactat catcatcaac ccggaactgt
     5161 tcggcgcgcc ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc
     5221 cgcatgatga cgataagccg attttgctcc cgggcgagtg ggaagtgaac acgcgtcgcg
     5281 aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt tgtgatgcgg
     5341 cgcggcagat tggtatgccg gaagagacgt tgcaggcttt ctgtcagcag ttagccagct
     5401 aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat caatgagggt
     5461 tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga cgtagcgtaa
     5521 acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg tagcgtcgca
     5581 tcaggcatct gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa ttacttataa
     5641 attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct atatccactg
     5701 gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg
     5761 ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc cgttaccggt
     5821 tccgcggcaa acacgccaaa tgacagagtt gaaagagcca tagcagcaac aacagtattg
     5881 atagttttca taattaatct ctcgcaggtg attttttgtt taagtgacgt tgtttcgtcg
     5941 atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg accgccttgt
     6001 tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg tacttatgga
     6061 cttgttttat gcagcgtggc agtcacagca gcgttaaagg cacggaatgc cacgcaaaat
     6121 acaaattata ctcgctatgt atctcgccag ttgtagttaa cgtaactggc tgtctttcga
     6181 acctctgcga ttatatcctg tataagcttg tttttgtaaa tctttctcct gctgacaatg
     6241 aatgcataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg gggcaccgca
     6301 ctcttcacat tcatacaggc tttcgccacg cggaatttca ccccgagcgc gggcaatcgc
     6361 atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc aaccggatgc
     6421 catatcgtcc tccccatatc aatacttgta cagttaagtg tagctaatat agggacgaac
     6481 tcgggcagtt caagcatcag atctccgacc attcccgcag cagattatga taaagattaa
     6541 gcagcgacag gatctcttca ctttcgccgt agcggctttt cagcgactga atattgttgt
     6601 ccagttcaaa cagcatggcg cgctttttat catcacggat catcgactgg atccacataa
     6661 atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg ctggagggat
     6721 acaacaccag atcgcctgcc gggagtttta cccgatgttg tccgaaggtg tcattaacga
     6781 ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca gaaaggtcag
     6841 tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg aaaccatagg
     6901 tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc aaggccgcgg
     6961 caaagaacaa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg gcggcgtata
     7021 acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt gcgccagtgg
     7081 tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaacgg gcgacgtcct
     7141 gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa gtggggcctg
     7201 cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc ttggctcgcc
     7261 cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat caaacaggtt
     7321 gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt tggcatcggc
     7381 gacccagtaa ccttctgtaa acgctggcgt tcccaccgcg ccgtctgaac ctttatgcat
     7441 actgccgata tagcgtgcgc ccgcgccaac agagatatct tcggttgcct gatattggct
     7501 ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat cctgggcaac
     7561 atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca cctgccacgc
     7621 gggagtgata ttccctgcca cggatatctc atagccttcg acgcgtttct taccgtattg
     7681 cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg atatcagtac ggaacagcgc
     7741 ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct cgctggtgtt
     7801 ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccgctgccag actgcgcaag
     7861 ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat agacattgcc
     7921 gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc ccgacttggc
     7981 ggtgtcgacg gtggtgaccg gagaaccttt tgccacacca actgggcagg tgatggcacc
     8041 gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat tatccagacg
     8101 gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg cgtaaattgc
     8161 gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc cgggatgaat
     8221 gctgctgtca ggatgataaa tatttaccgc aggtaacgtc accggattaa cgccgtagtt
     8281 agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga tagaaccggt
     8341 atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca catctttagt
     8401 attcgccgtg cgtgaccagg tccagctatt cacatcgctg gtgggctggg taatattcga
     8461 cgccccaccc ataatcgccg tcatcaggta atcctgcttc acgcgcgacc aacgggtagt
     8521 gttgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtag cggtgtcggt
     8581 ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgcgtatcaa cttttccgga
     8641 atgattcagg gtcgccgtac ccgcagatgg ggcagaatag cccggcaaac cgatggttgg
     8701 aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga taattaagat acaaacgatt
     8761 cgctgtacca aggccaaaag cgacagaagg ggcgatgccg taacgctcat ttttgacttt
     8821 gtcgcgtccg gcatcgtgcg ttttttcgcc catcacattc aggcgcaccg cggtggtatc
     8881 accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc caatactggc
     8941 ggaggcgtca atgccagaat cattgcgcgg ctgcttgctg atcatattga tcgagcccgt
     9001 cggcgcgctg cgcccgtagt cggtgccgga aggcccttta atcacttcga cctgctcagt
     9061 attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat aaatactgtt
     9121 agaagtatca gcaccgcgca tataaatggc gtcgccagtg gtggagttac cgttctcacc
     9181 agcaaaaaat gcacccacgc cggggacgtt tttgagcgcg tcggtaaggt tggttgcgcc
     9241 ctgatcttta atcacttgtt cagaaattac cgtcatcgtg cgggtagtat ccgctaccgg
     9301 acgtgagaat ttcggatcgg cagattgttg tggcgcataa agcgaaggcg ttgatgcttc
     9361 gacaaccagc gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct gagccacagg
     9421 cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc tggcagggaa
     9481 attgcgattg ttttccattt tgcaggtgac tttttcttat atgttgaaat tttcactgcc
     9541 acgagaaagc ctctctgcgg atgcgatgcc actgttaatc tcgtgatagt gctcgtgcaa
     9601 gtgataataa ttttgataag cattatcact tggcgcgatt ttctatcacc cattgtgcga
     9661 aaaagaaata catttattta cattgcgttc atatttgatg ttttttgatt acaaaacaga
     9721 cgaaaactgc atccagaggc gaatgcagtt acgttggtgg ggttatttgt agatggttgc
     9781 agtaccgaac atctggtcat ttccgcctgc ggaattaatg acataacctt tcgcgccttg
     9841 ttcgcgcgct ttttctgcca gtttatcctc cagatcgctt aggtttgatg cgccagttgc
     9901 cgaaacggta ccggcgggcc gtagctgacc gctgtccagg ttactcatcg gttgcgcggc
     9961 ataagccgtg agagagattc cgctcaggac ggtggcaatc agtagcgtga ggcacttttt
    10021 cataatgaca tcctcataaa tacaacgggt agtgcctgat aagtgtaggt cgcctgcgcg
    10081 atgaaaatga cgcaaaaaat tgacagagat gtgcttgttt tttgaacgat atatttttac
    10141 aaaataagat aaatcaatga catgcataat gctgattttt atgatgaatt gccataaacc
    10201 atagccatgt gaaggtgttt agaaatgata aacgcagcca gatatttacc ctgtcgcgtt
    10261 atgcgggtat cttacgccgc tgcttaaagg agaatgctat gtccgcccag aaaccggggt
    10321 tgcatccgcg caaccgtcat cacagccgct acgatctcgc cacgctttgt caggtcaatc
    10381 ctgaactcaa gcaattcctc acgcttacac ccgccgggga gcaaagcgta gactttgcca
    10441 atccgctggc ggtgaaggcg ctcaataagg cgttgctggc ccatttttac gccgtagcga
    10501 actgggatat cccggacggt tttctctgcc cgccagtacc gggccgggcg gattatattc
    10561 atcaccttgc cgatttactg gctgaagcaa gcggaacaat tccagctaat gccagcattc
    10621 ttgatatcgg cgttggtgcg aactgtattt atccgctgat tggcgtacat gaatatggct
    10681 ggcgttttac cggtagtgaa accagcagcc aggcgttaag cagcgcgcag gcgatcatca
    10741 gtgctaatcc ggggcttaat cgcgccattc gtctgcgtcg gcaaaaagag agtggggcga
    10801 tttttaacgg catcatccat aaaaacgagc aatacgacgc gaccttgtgt aacccgccat
    10861 tccacgattc tgccgctgcg gcgcgggcag gtagtgagcg taaacgccgt aacctggggc
    10921 tgaacaaaga cgatgcactg aactttggtg gccagcaaca ggagttgtgg tgtgaaggcg
    10981 gtgaagtcac ctttatcaaa aagatgattg aagagagcaa aggcttcgcg aagcaggtga
    11041 tgtggtttac ttcactggta tctcgtggtg aaaacttacc gccgttgtat cgtgctctga
    11101 cggacgtggg cgcggtgaag gtggttaaaa aagagatggc ccaggggcaa aagcagagtc
    11161 gctttattgc ctggaccttt atgaacgacg agcagcgccg ccgttttgtt aatcgccaac
    11221 gttaaagcgt tggctccccc ggcggcagcg gttcagccgg gggcgcgccc ggagcaggca
    11281 gcacctgata agtctgcact ggcgcgcgaa cgcccgccag gtcgaaatgt tttttcacct
    11341 ggctgtcgag ggcaaagcgt accgtccact gtttgagcgg cagcgtagtg aacgaaacac
    11401 gcagtgtaaa cgcggtattg cttaagccga caatcccggc aaaattcggt tcaccaataa
    11461 tcagcccgcg aatttcttcg ttttccatta attccgctac cgcatctttc agtgcctgat
    11521 tggctttatc agcatcttca tggcgatcaa catcgtagtt tgccactacc gaaccaatgc
    11581 cgcggacaaa gttggcaaag gtggtaatcg aagaccacgg aatgatgtgg tacgccccgg
    11641 tatcctgacg tacgcccacg gagcgaatcg acatccgttc cacagtgccg gtcaacggcc
    11701 caatagtcac caaatctcca gtgttcatgc cgttttcaaa ctgaataaat accccggtga
    11761 taatatcttt caccagcgtt tgcgaaccaa acgagatagc cagacctaat gccccggcac
    11821 ctgccagcaa tggcgcgata ttgacgccga tttccgacaa cacaatcatg atggtgatgg
    11881 tactgataat caccgccagc gcgttacgaa acagcgtaag cagggtacgc gtacgggcgc
    11941 tgggtagcgg gcggccatga atatccgaag ccagccggtt ttcgatcaaa ctggcgagca
    12001 ccgtccagcc aaccgccgag aagaaaagaa taagtgcgat gcggatcagg atatctacgg
    12061 ttttctgccc cgcgccgttt tgcagccagt tccagaaatc gaacaatccc catgcgctca
    12121 acagcagcat tacagccacg cagacggtca gaatacgcgc cgttttcagc gccgccgaaa
    12181 gccagccatt cagccgtttt tgcaactccg gatagttacg ctgagtatgt ggcgagagag
    12241 tgatggtttt tgccagccag cgggaaaaca taccggaaac aaacgctgcg ataccaataa
    12301 tcgccaggct gcgcaccgtt gcacccatca taaatttcag gctgttgccc ggatcgaaca
    12361 acgaaaagaa aaacagcacg ataaaatagg cgcttgccag ccagtgccac accagcgcaa
    12421 aggcgcggat aaacaggctg aaaaaggcca gcgaatgctc cgcaaagttg agcaaatgct
    12481 gggtaatctc ttttttatta cgaaagatca ggtacaacgc ccagacggtc atgcacagca
    12541 taatgatgac gttcgccagc gcacctatct gtacattcac ctgattagag ataatcggca
    12601 cggccacaat caggccataa cctatcaaac tgcttaacca gctcaggcga cgactccagt
    12661 aacgggcgct ctcgtcctga atcgtgaacg ggcgcagttc cgccacgttt gggcaaaaaa
    12721 tcaggcgtag tacggctttg aaaaattcaa tcagggcaaa ggcgttgaga aacaaacttt
    12781 gttggaaagc gatggtgcga ctgcctgcat tcaggttgtc gcttaatacc tggccgacaa
    12841 ataatgtcag ggccagtaac agcaggtcga taataaacgc cccgataatc atcgccggaa
    12901 gctgcaacca gttactgcgc tcacgatttt tttgccgcgc ccactggccc attttgcgat
    12961 acagcggcag tgcgcacaga cgaatcagcc agtaaaaacc aaacactaat accgctaaca
    13021 ttgaaaaatg ggtcagcgca ttactgaagg tttgtggatt aaacggctta tgcggggagc
    13081 cggtgatatt gcgataaagc tgcccgaagc gggcggaaag ggcctcacca taatggcggc
    13141 tgaattcggt gaccttttgc agcacggttt gctcttcaac cagcgtcggc ggcacgatct
    13201 tcgggaccgg ttcagcaggt ggcgtcgcgg cgacggtgcg caactggtcg atcaactctt
    13261 tacgcgaggt gtcattatcc agcacatccg ccagtgcgcc ataggccgct tttttttgtt
    13321 cgatatccgg ttccggggcc ggttcagtcg ttgagtccgt tgtcgttgtg gttgtaacgc
    13381 cgggtatgga taccgcgtgg gcaggtgcgc ccagcaggca gaagaggatg aacaggatcc
    13441 accgcatgac tcctccagtg agaaaacaga gcaaaaagat aagtatagat gctgggggag
    13501 gggcgatttt aaatgagagg aatctggtgt acctcccttt cgggtgaaag ggaggctgag
    13561 aagttaagag acgtgttgca aaaattcctg caagcgctgg ctcggcggat ttttgatcaa
    13621 cacctgcgga tcgccatctt ccgcaatccg gcctttgtcg ataaagatca ggcgcgaagc
    13681 tactttctcg gcaaaaccga tttcgtgggt cacgatcacc atcgtcatcc cttcttcagc
    13741 cagatcctgc ataaccttca gcacttcatg gcgcagttcc gggtcaagag cggaagtcgg
    13801 ttcatcaaac agcatcattt tcggcttcac cgccagcgcg cgggcaatcg ccacacgctg
    13861 ctgttgacca ccagaaagtt cggaagggta gtgatgtgca cgttctgcca gaccgacttt
    13921 cgccagcagc tcacgtgcca gtttttccgc ctcttctttg ttcgcgccac gcacgcgcag
    13981 cggaccaaac atgacgtttt ccagcgccgt cagatgcggg aagaggtaaa actgctggaa
    14041 caccatacct gcttcctggc gaatcaggcg ctcgtcaact ttcggatcgt taaccttcag
    14101 gccatcgaca atcagatcgc cggaggtgat ttcttccagt ttgttgatgc agcgcagcag
    14161 ggtcgattta ccggaaccgg acggcccgat aatcaccacg acttcgccct gggcaatgtt
    14221 caaatcgata ttgtgcagca cctgggttgg gccaaagtgc ttggagacgt ttttaaattc
    14281 aatcacagga ttttcatcct tctttccaga cgacgcagaa taaagctcag caccagggta
    14341 ataatcagat agaacaccgc cacggcgctc cagatctcaa gggcgcggaa gttaccggca
    14401 ataatttctt gcccctgacg ggtcagttcc gccacgccga taacaataaa cagcgaggtg
    14461 tctttaatgc tgatgatcca ctggttaccc agcggcggca gcatacgacg cagtgccagc
    14521 ggtaaaatga cgtagcgaat ggtttcccaa cgtgaaagac cgagcgccag tcctgcttca
    14581 cgaaaacctt tgtggataga cagcaccgca ccacgcgtga tttccgcaat gtacgcgccg
    14641 gagttgatca tgatggtgac caccgccgca gtaaatgggt cgatgcgtaa gtcgttaaac
    14701 gccatcggca gggcaaaata gataaacatc acctggacga cgataggtgt gccgcggatc
    14761 acttcaataa agaccagcgc gacgtggttg gctatccaac caccgaaggt gcgtgcaaaa
    14821 cctgccagca atccgattac cagaccgcca gccagaccga ggaccgaaat ccacagggtc
    14881 attttggcac cttcaatcag aagcggaatg gcaggccaga tggcactcca gtcaaactgc
    14941 atatgttgtt cctgttaccg tggtgtaaat aatcaaattc agggtaatta atggccccgg
    15001 agcgggtttg cgccctcacc ctaatcctct ccccagaggg gagagggaac tgccagtgcg
    15061 ctttacaggt gtagcgttat tatttcggtt cagtaccgaa ccattttttg tagatttcgt
    15121 tgtaagttcc gttctcgcgc agggttttca acgcgccgtt gactttgtca cgcagctcgt
    15181 cgctaccttt cgggaacgct ataccgtatt gctgcgcttc cagagagtca cctaccgctt
    15241 tgaactgacc gttaccggcg gttttgatga agtacagaat gtttggcgta tcgtgcagaa
    15301 cggcgtctgc gcggttggtg cccagttcca tataggcgtt atcgatgttc gggaactgac
    15361 gcagatcttt agttttgatg tttgctttcg cgtaatcaac ggagccagta ccgctcttca
    15421 cagcaaccac tttcccgtcg agatctttca cgcttttcac atcgttattg ttagctttca
    15481 ccatcactaa caggccgctt ttgtagtagc cgtcagagaa atcgatcgct tttttacgct
    15541 cgtcggtgat ggtaatgccc gccagcgcca gatcgacgtt tttggtttgc agtgccggaa
    15601 tgatcccact gaaatccatc ggcttcagtt cgtaatccag cttcagctct ttagcgatgg
    15661 cagcccacag atcaacgtca aagcccacat atttatcgcc ctgtttaaat tcaaacggaa
    15721 cgaaggcggt atccgtcgcg acaactaatt ttttatccgc ggcatgagaa gaaaccgcaa
    15781 aagccagggt cagtgcagcc agtgaaactt ttaatacaga cttcatagca tttccttttt
    15841 tctatccacg ggacgatccc ctgcgtgaca ttcacatata tgaaaaaatc gtgccagttt
    15901 tgcaactcat tgatttttcg agatgcggca aaaacgtgat gcacgattta tagggcaata
    15961 ccctgaagat gcaccattct ggggcaccaa actggtgcgc taaaattgtg cactcaacac
    16021 agtgattatt tagcgtaaaa cctgatgaaa aaacaatctt tatgtaacga ttgtgtgatg
    16081 atgtggatac aaaaaattta cttaatcagc tggggatagc agatggatgc actaaataag
    16141 tgcgttgagg cgggctgcaa aacaaaacgg cctcctgtca ggaagccgct tttatcgggt
    16201 actaaagttc tgcaccatca gcgatggatt tattcgatgt tagactcgat aaaccacagg
    16261 aatttatcca ggtcgcgaga cgcggcggtc aggatatctg cggtgtcgtc atctttcgct
    16321 tcgccaatcg ctttgcgtac gtcattagcg acgattgcgt aacggtcagc cagttctttc
    16381 aggtgatcct gaacgttgtg gatgtccagc gggtaacttt tcagcggggt tttgctgttg
    16441 ataacttgag tggtccccag agctacaccg cctagctgca ctgcacgttc tgccatggta
    16501 tccagatgat cgatcagtgc agtgcggaag ccatccagca tttcatgtac ggcaatgaag
    16561 ttagcgccgc gcatgttcca gtgcgcttgt ttggtaatca aagaaagatc aataaactgg
    16621 ataacctggc gattcagcaa ctctactgtt gcttttttct cgctgtcaga gacatcgttg
    16681 cgggtataaa gcagattggt cgcttttgat ttaactaatt tagcggtact cataatttca
    16741 tatcctcttg atgttatgtc ccagtaatta acgagactaa gtatagcacc ggctatgtgt
    16801 tccgctattc tggctgttcc tatcacacta atagtggtaa caagcgtgaa aaacaaaact
    16861 aat
//
LOCUS       DAZUGK010000098        16613 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZUGK010000098 DAZUGK010000000
VERSION     DAZUGK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.098"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            186..338
                     /gene="mokC"
                     /locus_tag="ACHY5W_002749"
     CDS             186..338
                     /gene="mokC"
                     /locus_tag="ACHY5W_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFS4447625.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(442..1572)
                     /gene="dnaJ"
                     /locus_tag="ACHY5W_002750"
     CDS             complement(442..1572)
                     /gene="dnaJ"
                     /locus_tag="ACHY5W_002750"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4447626.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1661..3577)
                     /gene="dnaK"
                     /locus_tag="ACHY5W_002751"
     CDS             complement(1661..3577)
                     /gene="dnaK"
                     /locus_tag="ACHY5W_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4447627.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3954..4358
                     /gene="yaaI"
                     /locus_tag="ACHY5W_002752"
     CDS             3954..4358
                     /gene="yaaI"
                     /locus_tag="ACHY5W_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFS4447628.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4384..5097
                     /gene="msyB"
                     /locus_tag="ACHY5W_002753"
     CDS             4384..5097
                     /gene="msyB"
                     /locus_tag="ACHY5W_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS4447629.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVLHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5246..5812
                     /gene="satP"
                     /locus_tag="ACHY5W_002754"
     CDS             5246..5812
                     /gene="satP"
                     /locus_tag="ACHY5W_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFS4447630.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5846..6433)
                     /gene="mog"
                     /locus_tag="ACHY5W_002755"
     CDS             complement(5846..6433)
                     /gene="mog"
                     /locus_tag="ACHY5W_002755"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFS4447631.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRYVSE"
     gene            complement(6548..7501)
                     /gene="tal"
                     /locus_tag="ACHY5W_002756"
     CDS             complement(6548..7501)
                     /gene="tal"
                     /locus_tag="ACHY5W_002756"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS4447632.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7780..9210
                     /gene="yaaJ"
                     /locus_tag="ACHY5W_002757"
     CDS             7780..9210
                     /gene="yaaJ"
                     /locus_tag="ACHY5W_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFS4447633.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9280..10056
                     /gene="yaaA"
                     /locus_tag="ACHY5W_002758"
     CDS             9280..10056
                     /gene="yaaA"
                     /locus_tag="ACHY5W_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFS4447634.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10209..10505)
                     /gene="yaaX"
                     /locus_tag="ACHY5W_002759"
     CDS             complement(10209..10505)
                     /gene="yaaX"
                     /locus_tag="ACHY5W_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFS4447635.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10719..12005)
                     /gene="thrC"
                     /locus_tag="ACHY5W_002760"
     CDS             complement(10719..12005)
                     /gene="thrC"
                     /locus_tag="ACHY5W_002760"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFS4447636.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTIRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12006..12938)
                     /gene="thrB"
                     /locus_tag="ACHY5W_002761"
     CDS             complement(12006..12938)
                     /gene="thrB"
                     /locus_tag="ACHY5W_002761"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFS4447637.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12940..15402)
                     /gene="thrA"
                     /locus_tag="ACHY5W_002762"
     CDS             complement(12940..15402)
                     /gene="thrA"
                     /locus_tag="ACHY5W_002762"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFS4447638.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15428..15547)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15483..15548)
                     /gene="thrL"
                     /locus_tag="ACHY5W_002763"
     CDS             complement(15483..15548)
                     /gene="thrL"
                     /locus_tag="ACHY5W_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFS4447639.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15762..16448)
                     /gene="yjtD"
                     /locus_tag="ACHY5W_002764"
     CDS             complement(15762..16448)
                     /gene="yjtD"
                     /locus_tag="ACHY5W_002764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS4447640.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3852 a         4420 c         4116 g         4225 t
ORIGIN      
        1 acagaatggg ttaaagagag cgatataata gcggcgggtg cttgaggcta tctgtctcaa
       61 gcattagctg aacggcagat agagaaaagc cccgagtgat attttactat caacccgagg
      121 ccccctatat gctgaacaca tgtagagtgc ctcttactga ccgtaaggtc aaggagaaga
      181 gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt atcaccgccg
      241 tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga actggccaga
      301 cggaggttgc tgttttcacg gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc
      361 ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc aggcctgcct
      421 acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct tcacaccatc
      481 aaagaagctc tttgagcgcg ggctgttgtg ctcaccggtt gggccaccga agctttcttg
      541 cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt cgacgacaac
      601 gcggcacagc aaatcaccct gtgcgccacc gcggacagac ttgacgcctt taccgcgcat
      661 acggaacagc ttaccggtct gggtttcgcc aggcactttc agtttaacgc gaccatcaag
      721 ggtcggtact tcgatttcac cacccagcgc cgccatagcg aagttgatcg ggacttcgca
      781 atacaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta
      841 cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg
      901 gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc gctcaacacg
      961 accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcggc cctgacagtg
     1021 tggacaggtc tgctgcacgg caaagaaacc ctggcgcatc tgcacctgac cagaaccatg
     1081 acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc
     1141 acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag cttcttcgag
     1201 ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc
     1261 gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc
     1321 aaaaccgccg ccgcccatgc caccttgctc aaacgcagca tgaccatact gatcgtatgc
     1381 cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt taaatttcgc
     1441 ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca ggcgtttgta
     1501 ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct cgtaataatc
     1561 ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag aggaaattcc
     1621 ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg tctttgactt
     1681 cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac
     1741 cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc tgggaaacct
     1801 gtgccagctc ctgcattttc gcttcgatag cggctttgtc ttcacctttc agagcagttt
     1861 ccagtgcagt cagtgcagac tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg
     1921 cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg cgagtctgta
     1981 ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt accattttct
     2041 ggatttcatc ttcgttcaga ccagaagaag ctttgatggt gatcttctgc tctttaccgc
     2101 tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg aaggtaactt
     2161 cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caagttgaac tgacccagag
     2221 atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt accgcagact
     2281 ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg gtgtttttcg
     2341 cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc ggggtaacgt
     2401 ccagcagcag tacgtctttt acgtcaccag tcagaacacc accctgaaca gcagcaccga
     2461 ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag aactcagcaa
     2521 ctttcttctg aaccattggc atgcgagtct gaccaccaac gaggataacg tcgtcgatat
     2581 cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg gaacggttta
     2641 ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc atgtgtttcg
     2701 gaccggtcgc atctgcagtg atgtacggca ggttaacgtc ggtctgctga gcggaagaca
     2761 gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc ggatcgttgc
     2821 gcaggtcaat gccctgatct ttcttgaatt cttcaaccag atagttgatc agacggctgt
     2881 cgaagtcttc accacccagg tgggtatcac cgttggttgc cagaacttcg aaggtttttt
     2941 cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccacca cccaggtcat
     3001 aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc gcagctgcgg
     3061 tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg tctttggttg
     3121 cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca gttaccggtt
     3181 cacccaggta atcttcagcg gttttcttca tttttttcag cacttcagca gaaatctgcg
     3241 gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca gcagcaataa
     3301 ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag cggcggccaa
     3361 tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga cgtttagccg
     3421 gctgaccaac cagagtttca ccatcctggg tataggcaat aatagaaggc gtggtgcgat
     3481 cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct acacaagagt
     3541 tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca ctatatattc
     3601 ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca actgcccaat
     3661 ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg taacccacgt
     3721 catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg cgatatggca
     3781 ggacaagacg taaaattgac agcaatttta ttcatataaa gaatgaatcg cagtcaaggc
     3841 attcatcaaa aaattgtaat aaaaagaaaa gattacgtgc ctgaatcttc tctttatcag
     3901 cagtaaacta gtgggtattc atccccctac ctcttcccac taagagaatc cttatgaaat
     3961 ccgtttttac gatttccgcc agcctggcga ttagcctgat gctgtgctgc acggcgcagg
     4021 caaacgacca taaactcctc ggcgtcattg caatgccgcg taacgaaacc aacgatctgg
     4081 cgctgaaact tcctgtttgt cgcattgtga aacgcataca actctctgcc gaccatggcg
     4141 atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc agtcagagcc
     4201 tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac attaacagtg
     4261 ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat
     4321 cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata gaaaccacaa
     4381 aaaatgaatg ttaattacct gaatgattca gatctggatt ttcttcagca ttgtagtgag
     4441 gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa aactcgcctc
     4501 tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc cgagcaacat
     4561 cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga tagtatcgcc
     4621 aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt ttcaaaacga
     4681 ttgaagctga aagccgacaa agagatgtct acgtttgaaa ttgagcaaca gttactggaa
     4741 caatttctgc gtaatacctg gaagaaaatg gacgaggaac ataagcagga gtttctgcac
     4801 gcggtcgatg ccagggtgaa tgagctggaa gagctgctgc cgctgctgat gaaagacaaa
     4861 ttattggcaa aaggcgtgtt gcatctgctt tccagccaac tgacccgcat tttacgcacc
     4921 cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg gcgcggggct gggaggccct
     4981 gtaggcgcgg cactaaatgg ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt
     5041 ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca ggtctgatac
     5101 cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg ccccactggc
     5161 tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat ttcaccattc
     5221 attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc cggcaccgct
     5281 gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg tgggttattt
     5341 cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg cgcaaatttt
     5401 tgccggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat tcacctctta
     5461 cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg gtctgaccga
     5521 tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat ttacgctgtt
     5581 tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa ttcgttttct ttagcctgac
     5641 cgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa tcatccactt
     5701 tgccggctgg attggtctga tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt
     5761 actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact aattgttttg
     5821 agatcccccc gctcgggggg atgttttatt cgctaacgta gcgtcttgca ctcttcggtc
     5881 tgaatgcagc aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc
     5941 agtacggtac gctggcaaaa ataccgtgta ccacaacgtt accctcagcg tccttcacac
     6001 cttccagcgt ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt
     6061 tgcgaatcac acccacctga cgcgaaagga tcgcagttgg tacaaaatgc aggctgatct
     6121 ggcgcatctg ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg
     6181 gcgttacgtc acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca
     6241 tttcatccac cagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc
     6301 gggtttccag ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgcaggga
     6361 tacctttatc ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac
     6421 gtaaagtatt catcaagatt tttccgttta aacagtctcg ttaaacagaa tgatacactg
     6481 cgaagggagt gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta
     6541 agaatgatta cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact
     6601 tacggatacc ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga
     6661 actcggactc agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac
     6721 gttcgatagc cccttcgctc tcagccagct ctttcagcag tgccggtgcg atggtcagac
     6781 ggtcgcagcc tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca
     6841 cggtttcata accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg
     6901 gatcttctgc cggagcgtac tctttcttat cggtattcgc tttgtaccag tcaagaatac
     6961 ggccaacaaa cggcgagatc aggaacacgc ccgcttccgc acaagcacga gcctgagcga
     7021 aggagaacag cagggtcagg ttacagttga tgccttcttt ttccagctgt tctgcagcac
     7081 ggataccctg ccaggtagaa gccagtttga tcagaatacg atcgttgcta atacctgcat
     7141 cgttgtagag tttgatcagg cgttttgctt tcgcaattga cgcttctgtg tcataggaaa
     7201 gacgcgcatc aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa
     7261 tatttactgc cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct
     7321 gtttcgccca ggcgacagca tcatcaatca acttacggta ttccggaatc tgcgctgcgt
     7381 taagaatgag agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga
     7441 tgtccccagt gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca
     7501 tgatagtatt tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata
     7561 tcacgacgca acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc
     7621 ggatttctga cactgcgttg atatcgcccg ccatttttat acaaaacctc atgtatgcta
     7681 cgcagaagtt atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac
     7741 acaggaaagt catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt
     7801 attaacagcg tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg
     7861 ttcacttttc gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt
     7921 aaaaatagca ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt
     7981 cttgcggcgc gcgtgggtag cggcaacctg gccggcgttg cgctggctat taccgccggt
     8041 ggacctggag ccgtcttctg gatgtgggtt gccgcgttta tcggcatggc gacctcgttt
     8101 gccgaatgtt ctctggcaca actttataaa gaacgtgacg tcaatgggca gtttcgtggc
     8161 ggaccggcat ggtatatggc gcgcgggctg gggatgcgct ggatgggcgt tctgttcgcc
     8221 gtctttttgc tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc
     8281 cgagccctga gtttttcttt tgattttccc ccgctggtga caggcattat tctcgctgtc
     8341 tttgctctgc tggcaatcac tcgcggtctt catggcgtcg cccggctcat gcaagggttt
     8401 gtcccgttga tggcgataat ctgggtgctg accagcctgg tgatttgcgt aataaatatc
     8461 gggcaacttc cccacgtcat ttggtctatt tttgagagtg cttttggctg gcaggaagcg
     8521 gcaggcggcg cggcgggata taccttaagc caggcgatta ctaacggttt tcagcgcagt
     8581 atgttttcca atgaggcggg aatgggttcg acgccaaacg cggcagcggc agcggcgtcc
     8641 tggcctccgc atccggcagc gcaaggaatt gtccagatga ttggcatttt tatcgacacc
     8701 ctggtcatct gtacggcaag cgccatgctg atattactgg cgggtaacgg cacaacctac
     8761 atgccgctgg aaggtattca gcttatccag aaggcgatgc gggtgctaat gggttcctgg
     8821 ggtgctgagt ttgttaccct cgtggttatt ctgtttgcct tcagctccat cgttgccaac
     8881 tacatttatg ccgaaaacaa tctcttcttt ttacgcctga acaaccctaa agcgatctgg
     8941 tgtttgcgga tctgcacctt cgcaacggtc atcggcggca ccttgctaag tcttccgctg
     9001 atgtggcaac tggcagatat cataatggcc tgcatggcta ttaccaattt gaccgccatt
     9061 ttactgctct cgcctgtggt tcataccatt gccagtgatt atctacgcca gcgtaaactc
     9121 ggcgtgcgcc cggtgtttga tccgttgcgt tatccggata tcggtcgcca gctttctcgg
     9181 gacgcgtggg atgatgtttc gcaggagtaa tcacaactat cgatcaactc attctctttt
     9241 tttgctaaag tcggcataaa tttcctgcaa ggactggata tgctgattct tatttcacct
     9301 gcgaaaacgc ttgattacca aagcccgttg accaccacgc gatatacact gccggagctg
     9361 ttagacaatt cccagcagtt gatccatgaa gcgcggaaac tgacgcctcc gcagattagc
     9421 acgctgatgc gtatcagcga caaactggca ggtatcaacg ccgctcgctt tcatgactgg
     9481 cagccagatt tcacgccgga gaatgcccgc caggcgattc tggcgtttaa aggtgatgtc
     9541 tacaccggct tgcaggccga aaccttcagc gaagacgatt tcgattttgc ccaacagcat
     9601 ttgcgaatgc tttccggctt gtatggcgta ctccgcccgc tcgatttaat gcagccttat
     9661 cgtctggaaa tggggatccg tcttgagaat gcccgaggga aagatctgta tcaattctgg
     9721 ggagatatca tcaccaacaa gctgaacgag gcgctcgcag cacaaggcga taatgtggtg
     9781 attaatctgg cgtcagatga gtattttaaa tcggtgaagc cgaagaaatt gaatgccgag
     9841 attatcaagc cggtgttcct cgatgagaag aacggcaaat ttaagataat cagcttctac
     9901 gctaagaaag cacgcggtct gatgagtcgt ttcattattg aaaatcggct gaccaaacca
     9961 gagcaactga ctggttttaa tagcgaaggt tacttctttg atgaagcttc ctccagcaat
    10021 ggcgaactgg tgtttaaacg ctacgagcag cgttaagttt gtagtcaatg ccagatgcgg
    10081 cgtgaacgcc ttatccggcc tacaaaagca tgcagattca atatattgca gaattcacgt
    10141 aggcctgata agacgcgcca gcgtcgcatc aggcgctgaa tgccggattg ttacccggca
    10201 tttgtcattt agcggtgatg tttgcctgga ccatgaccgc cgtgatgatc atgatgagct
    10261 ttcttatggt gacgcggcgg tggcggcggt ccgtgtgggt gccagcgatt gcctcgccat
    10321 tcataatgtt gtttccacca gccgtggtcg cgccagtgac cgccatccca gtaataaccg
    10381 cgattatcac gatcgcctat ctgtaatttt actgacggga ctaacgtaat ttccgcagcc
    10441 tgcgttgcca tgggagcgac cagaaccagg gaaagtgcga gtacgataga ttgcatcttt
    10501 ttcacaggtt actcctttta tcattgcccg ttatgggtcg atatccggag attacgcgat
    10561 gagaacggcg gttgttattc tccgcaatcc taatctctaa gttaccgcat ttattgagaa
    10621 tttctccttt ttccctgtcg tttctctcca tattttcttc acaaaaaagc cgggctgcat
    10681 aaaagcaaac ccggcctgat tgagataatg aatagatgtt actgatgatt catcatcaat
    10741 ttacgcaacg cagcaaaatc ggcgggcaga ttatgcgaaa gcaagggtaa atcggcacgt
    10801 tctgccagct cttttggcag atccaacgtt tcaccgagaa tcgcttccac gctctcttta
    10861 aatttcgccg gatgcgcggt gccgaggaac aagccatatt cgcctggatt caactggtca
    10921 cgcagcgcac gatacgctac ggcagcgtgc ggctccgaag tgtagcccag ttcttttaac
    10981 tcacgtattg tctgttgcgt ggtttcatca tccacggctg cataacccag ctctttcagt
    11041 tgccagattt tgcggcggaa caactcttcc acacgcggcc agttgttcgg ctgactcacg
    11101 tccatcgcgt tggataacgt cgcctgagtc gctttgggtg accactgacc gtcatgcagg
    11161 aaacgtggca cggtatcgtt cacgttggtc gcagcaataa aacgtttcac cggcagaccg
    11221 agtgacttcg ccagcagacc cgccgtcaaa tcgccgaagt ttccgcttgg caccgagaca
    11281 accagctggt tgcgcgcttc ctgcggcagc tgcgcaactg cttcaaagta gtagcaaatc
    11341 tgcgccagca accggctgat gttaatcgag ttagctgagt ttaaccccag cgccactttc
    11401 agctcttcat catcaaacgc ctgcttcacc agcgcctgac aggcatcgaa atcgccgtcg
    11461 atggcaacag tttcgatatt gccgcccaat gtacagaaca gtttttcttg cagtggactg
    11521 attttgcctc gtggatagag gataaccact ttcacattcg gtaaaccgta gaaagcatga
    11581 gccactgccg ctccggtatc accggaggtc gcggtcagaa tggtcactgg cttatcgccc
    11641 gcaatatggg tcagcatttg tgccataaag cgaccgccga aatctttaaa tgccagcgtt
    11701 ggcccgtgga acaattccag acaaccgaca tcgctttcaa cattggcgac cggagccggg
    11761 aaggcaaacg ccgcgcgcac gcgctcttcc aggatttcct gcgggatttc atcaccaata
    11821 aacgccgaga ggatcttcgc actgcgggtg acaaaatcca gcttcagcat ctcatcaatt
    11881 tcagtcaggc tgaattccgg caggtcgtgc ggaaaaaaca gcccctgatt tttgcccaac
    11941 ccctgggtta cggcttgcgc aaagctgacc tgctcattgt gatctttaag attgtagagt
    12001 ttcatttagt tttccagtac tcgtgcgccc gccgtatcca gccggcaaat atgaacaaaa
    12061 ccttcctgat tttgcaggta gttcttaccc aaccagtcgg caacgcgctg ggcggtatcc
    12121 ggcttgtcac acagcgcgaa caaggtcggg ccggagccgg agataccgct cgctaccgcg
    12181 ccgatttccg cgaccgcctg ccgcgcctgc cggaagccag gcagtaaccg ttcacggtag
    12241 ggttctgcga taacatcttt catcagcttc gcggcaagct caggctgacg ggaatagcag
    12301 gcgtgaatga agcctgccag atgtcgcccg tgcgcaatgc aatcctggcg gcgatactgc
    12361 gccggtaaaa tagcccgggc ttctgccgtc gagactttaa tccccggata cgccagcacc
    12421 cacagccact catcaaaccc tggcacttgc tggctgatga tgtcgttttc ttcgatcatc
    12481 aactgcatac caccaagaaa acacggtgcc acgttgtcgt aatgtatgct gccggagatt
    12541 cgtccttcca gctcacccat caaagccagc aaacgagtgt cattaagcgg cttgccacag
    12601 tgttcattca tcgccatcag cgccgcgacc accgaacagg cgctggagcc taagcccgaa
    12661 ccgatcggca tattcttttc cagagtcatc gccactggaa tttgcttccc aagctcctgg
    12721 caaaaacgct cccagcactg ataaacgata ttttcccgtg gttctgacgg cagcttatcg
    12781 gcaaagcgtc ctaggttgtt gagactgaat gtctctgccg cctcaaccgt gactacatct
    12841 ccgagcaatg caccatcaac gggtgtcacc gccgccccga gcacatcaaa cccgacgctc
    12901 atattggcac tggaagccgg ggcataaact ttaaccatgt cagactccta acttccatga
    12961 gagggtacgt agcagatcgg caaagacacc ggcagcggta acgtcattgc ccgcaccata
    13021 tccgcgcagc accaacggca gcggctgata atagtggcta tagaaggcca gggcgttttc
    13081 gccatttttc actttgaaca gcggatcatt accatccact tcggcaatct tcacgcggca
    13141 gacgccatct tcatcaatat tgccaacata gcgcaaaact tttccttcat cacgggcctt
    13201 cgccacgcgc gcggcaaaga gatcgtcgag ctgtgacaga ttcgccataa aagcggcaac
    13261 atcaccctcg gcgttaaact ctgcgggcag cactggttca atttcaatat ccgccagctc
    13321 cagttcacgt cccgtttcac gagcgagaat caatagttta cgcgctacat ccataccaga
    13381 aagatcatct cgcggatccg gttcggtata acccatttcc cgcgccagcg tagtcgcctc
    13441 ggagaaactc atgccttcgt ctaacttgcc gaagatataa gaaagcgaac ctgaaagaat
    13501 gccggagaac ttcatcaatt catcaccagc attgagcaga ttttgcaggt tctcaataac
    13561 cggtaatcca gccccaacgt tggtgtcata gaggaattta cgccgcgatt tttccgccgc
    13621 atgacgcaac agatggtagt aatccatcga cgaggtgttg gcctttttgt tcggcgtgac
    13681 aacgtggaaa ccttcgcgca ggaagtcggc atattgatcc gccactgcct ggctggaggt
    13741 gcagtcaaca atgaccgggt tcagcagatg atattctttc acgaggcgaa ttaagcgccc
    13801 gagattaaac ggctctttgg cttgcgccag ttcttcctgc cagttttcca gatttaggcc
    13861 atgcacattg gtgagcagag ccttcgagtt agcaacgccg cagacacgta agtcgatatg
    13921 tttattcttc agccagcttt gttgacgctt cagttgctcc agcagcgcac cgccaacgcc
    13981 accgacgcca atcacaaaca cttcgataac ctgatccgta ttgaacagca tctgatgagt
    14041 aacgcgcacg ccagtggtcg catcatcgtt atttaccacg acagagattg agcgttcaga
    14101 agatccctga gcaatggcga caatgttgat attggcgcgg gccagcgcgg caaagaattt
    14161 cgccgagatc ccacgcaagg tgcgcatacc atcacctacc accgagataa tggccagccg
    14221 ttccgtcacc gccagcggct ccagtaagcc ttctttcagt tccaggtaga actcttcctg
    14281 cattgcccgt tcagctcgca cacagtcgct ttgcggaacg cagaaactga tactgtattc
    14341 ggaagatgat tgcgtaatca gcaccacgga aatacgggcg cgtgacattg cagcaaagac
    14401 gcgcgccgcc atgccgacca ttcctttcat ccccgggccg gaaacgctga acattgccat
    14461 gttattcaga ttggaaatgc ccttgaccgg taattcgtct tcatcacggc tggcaccaat
    14521 tagcgtacct ggtgcttgag gatttccggt atttttaatc aggcaaggga tctggaactg
    14581 ggcgatgggg gtaatggtgc gggggtgaag aactttagcg ccgaagtagg aaagctccat
    14641 cgcttcctgg taggacatcg acttcaacaa cctcgcatcg ggcacctgac gcgggtcgca
    14701 ggtatagacc ccgtcaacgt ccgtccaaat ctcgcaacaa tcggcgcgta aacaggcagc
    14761 cagaaccgcc gcggagtagt cggaaccgtt gcgtccaagc accaccagtt cgcctttttc
    14821 attaccggcg gtgaaacctg ccatcagcac catgtgatca gccggaatac gacttgccgc
    14881 aatacggcgg gtggactcag caatatcgac ggtagattcg aggtaatgcc ccactgccag
    14941 tagtttttcg accggatcga taacggtaac gttgtgacca cgcgcttcta acacgccggc
    15001 cataatggcg atcgacattt tctcgccacg gcaaatcagc gcagcgttaa tgctatccgg
    15061 gcactgccct aacaaactaa tgccatgcag gacatgtttt atttgggcaa attcctggtc
    15121 gacgaaagtt ttcaattgcg ccagcgggaa ccccggctgg gcggcggcga gtcccgtcaa
    15181 aagttcggca aaaatacgtt cggcatcgct gatattgggt aaagcatcct ggccgctaat
    15241 ggttttttca atcatcgcca ccaggtggtt ggttattttg gcgggggcag agaggacggt
    15301 ggccacctgc ccctgcctgg cattgctttc cagaatatcg gcaacccgca gaaaacgttc
    15361 tgcatttgct actgatgtac cgccgaactt caacactcgc atggttgtta cctcgttacc
    15421 tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg
    15481 cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg
    15541 cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg
    15601 gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc aggtaaccag
    15661 ttcagaagct gctgtcagag actctttttt taatccacac agagacatat tgcccgttgc
    15721 agtcagaatg aaaagctgaa aaatacttac taaggcgttt tttatttggt gatatttttt
    15781 tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg
    15841 cgttgttgta accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat
    15901 gccatggctc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc
    15961 gtcggttgtt gtattaatgt tgctaattga tagcaataga ccatcaccgc ctgccccaga
    16021 ttgagcgaag gataatccgc caccatcggc acaccagtaa gaacgtcagc caacgctaac
    16081 tcttcgttag tcaacccgga atcttcgcga ccaaacacca gcgcggcatg gctcatccat
    16141 gaagattttt cctctaacag cggcaccagt tcaactggcg tagcgtagta atgatatttc
    16201 gcccgactgc gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcagccagc
    16261 gtcgggaaaa ctttaatatt atcaataata tcaccagatc catgtgcgac ccagcgggtg
    16321 gctggctcca gatgtgcctg actatcgaca atccgcagtt cgctaaaccc catcgttttc
    16381 attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcgaccag aataatcgtt
    16441 atacgcatat tgccactctt cttgaccaaa tgaccgcgaa cagggtgatc actgtcaact
    16501 tattacgcga tacgaattta caaattctta acgtaagtcg cagaaaaagc cctttactta
    16561 gcttaaaaaa ggctaaacta tttcctgact gtactaacgg ttgaattgtt aaa
//
LOCUS       DAZUGK010000099        16236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZUGK010000099 DAZUGK010000000
VERSION     DAZUGK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.099"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(133..858)
                     /gene="phoU"
                     /locus_tag="ACHY5W_002765"
     CDS             complement(133..858)
                     /gene="phoU"
                     /locus_tag="ACHY5W_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4447641.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(873..1646)
                     /gene="pstB"
                     /locus_tag="ACHY5W_002766"
     CDS             complement(873..1646)
                     /gene="pstB"
                     /locus_tag="ACHY5W_002766"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4447642.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1829..2719)
                     /gene="pstA"
                     /locus_tag="ACHY5W_002767"
     CDS             complement(1829..2719)
                     /gene="pstA"
                     /locus_tag="ACHY5W_002767"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4447643.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2719..3678)
                     /gene="pstC"
                     /locus_tag="ACHY5W_002768"
     CDS             complement(2719..3678)
                     /gene="pstC"
                     /locus_tag="ACHY5W_002768"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFS4447644.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3765..4805)
                     /gene="pstS"
                     /locus_tag="ACHY5W_002769"
     CDS             complement(3765..4805)
                     /gene="pstS"
                     /locus_tag="ACHY5W_002769"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4447645.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKNFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5119..6948)
                     /gene="glmS"
                     /locus_tag="ACHY5W_002770"
     CDS             complement(5119..6948)
                     /gene="glmS"
                     /locus_tag="ACHY5W_002770"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4447646.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPNRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVGSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7110..8480)
                     /gene="glmU"
                     /locus_tag="ACHY5W_002771"
     CDS             complement(7110..8480)
                     /gene="glmU"
                     /locus_tag="ACHY5W_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4447647.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8833..9252)
                     /gene="atpC"
                     /locus_tag="ACHY5W_002772"
     CDS             complement(8833..9252)
                     /gene="atpC"
                     /locus_tag="ACHY5W_002772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4447648.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9273..10655)
                     /gene="atpD"
                     /locus_tag="ACHY5W_002773"
     CDS             complement(9273..10655)
                     /gene="atpD"
                     /locus_tag="ACHY5W_002773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4447649.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10682..11545)
                     /gene="atpG"
                     /locus_tag="ACHY5W_002774"
     CDS             complement(10682..11545)
                     /gene="atpG"
                     /locus_tag="ACHY5W_002774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4447650.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11596..13137)
                     /gene="atpA"
                     /locus_tag="ACHY5W_002775"
     CDS             complement(11596..13137)
                     /gene="atpA"
                     /locus_tag="ACHY5W_002775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4447651.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(13150..13683)
                     /gene="atpH"
                     /locus_tag="ACHY5W_002776"
     CDS             complement(13150..13683)
                     /gene="atpH"
                     /locus_tag="ACHY5W_002776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4447652.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLKALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13698..14168)
                     /gene="atpF"
                     /locus_tag="ACHY5W_002777"
     CDS             complement(13698..14168)
                     /gene="atpF"
                     /locus_tag="ACHY5W_002777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4447653.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14230..14469)
                     /gene="atpE"
                     /locus_tag="ACHY5W_002778"
     CDS             complement(14230..14469)
                     /gene="atpE"
                     /locus_tag="ACHY5W_002778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFS4447654.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14516..15331)
                     /gene="atpB"
                     /locus_tag="ACHY5W_002779"
     CDS             complement(14516..15331)
                     /gene="atpB"
                     /locus_tag="ACHY5W_002779"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4447655.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15340..15720)
                     /gene="atpI"
                     /locus_tag="ACHY5W_002780"
     CDS             complement(15340..15720)
                     /gene="atpI"
                     /locus_tag="ACHY5W_002780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFS4447656.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3912 a         4393 c         4049 g         3882 t
ORIGIN      
        1 tgtactgggg agagtttcga ggtgagtttg tgtggcaata tttcagtgag aaagtttatc
       61 agcccggtaa ctatgtatga ccgggctgat tgccagatgc ggcaaaaagc tttatctggc
      121 ctacagttag cactactctt tggggtcttt tcccgccagc agtttatcca gctcatcacc
      181 accgacgtga cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg
      241 gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa gtacgctcgg
      301 aatggtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtactc
      361 ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc
      421 gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact
      481 taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc
      541 cgccacgtcg ccaatacgct ccagttcggc aatggtttta ctgatcacca taaccaatcg
      601 caggtcgctc gccgtcggct ggcgtttggc aataatgcgc acgcaggctt catcgatcgc
      661 cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc
      721 ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt
      781 catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa tatgtttatt
      841 aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct
      901 tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat
      961 tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg
     1021 gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg
     1081 gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg
     1141 gcgataccac gcgcaatgca cagacgctgt tgctgaccac cagagagaga gtaaccgctc
     1201 tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgtacg
     1261 cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa ggcgatgttg
     1321 tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc
     1381 agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct
     1441 tctgcgcgct gctccgggta cagttcaaac attttgttga aggtacgcag cagcgtcgat
     1501 ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat atccaggttg
     1561 atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta
     1621 ctcggggcag tttcaaccat actcattgca atcttttcct catcgtgcct gatgcacttc
     1681 gctaatcagg cttaccgtag gccggatcag gcgtttacgc cgcatccggc aaagtgttaa
     1741 ccgtgtacag agtcaaaaag gcgctcggtc ggtcccctcg cccctctggg gagaggatta
     1801 gggtgagggg aaaaccgtgt cagcaatatc aaccgtgttt attcttcgca aaaacaacgc
     1861 gcgccagaat gttcagcagc agtacgcaca gggtaatgat caataccccg gcccaggcca
     1921 gttgctgcca ttccgcaaac gggctcatcg caaacttaaa gatcgtcacc ggcaggttgg
     1981 cgatcggttg catcatgtcc gtgctccaga actggttgga gagcgcggta aacagcagcg
     2041 gcgcggtttc accggcaata cgggcaatcg ccagcaggat accggtcata attccggaca
     2101 ccgacgcttt cagcgtaatc gcggagatca tcttccactt cggcgtaccc agcgcataag
     2161 ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt ctcggtggtg cggataacaa
     2221 tcggtacctg caacagcgcc agcgcaatca cacccgccca accggagaag tgctccatct
     2281 gcgccaccac aatggtgtaa acaaacagac caaccacaat cgacggcgca gagagcagaa
     2341 tgtcgttaat gaagcgaatc acttctgcca gccaggattt acgaccatat tccgccagat
     2401 aaatccccgc cataatgccc agcggcgtac cgaataccgt ggcccacaaa attaacagcc
     2461 cgctacctgc cagggcgttc gccagaccac caccttccgt attgggcggc ggcgtcattt
     2521 cagtgaacag agccagcgac ataccatcga taccgcgagt gatggtggac attaaaatcc
     2581 agatcagcca gaacagaccg aaggccatcg tcgccattga gagcgtcagt gcaatacggt
     2641 tcttgaggcg gcgacgcgcc tgcattttgc ggcgggattc agccagcgcc gcagtggttt
     2701 gcatttcaac catagccatt agcgtgcccc ctcattctta gccaggcgca taatcataaa
     2761 cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg atcaggccca gttccatcag
     2821 tgcggcaacg tgcagaccgg attccgcttc ggcaaattcg ttcgccagcg cagaggtgat
     2881 actgttgccc ggcatgtaca gcgaggcgct gtcgagctgg taggtgttac cgatgataaa
     2941 ggtcaccgcc atggtttcac caagcgcgcg gcccagcccc agcataatgc cgccgataac
     3001 accatttttg gtgaacggaa gaacgatacg ccagataacc tcccaggtgg tgcagccaat
     3061 accgtaggcc gactctttca tcatcaccgg ggtttgttcg aacacatcac gcattaccgc
     3121 cgcaatgtac ggaataatca tgatggcgag gatcacgcct gccgcgagga taccgatacc
     3181 aaatgccggg ccagagaaca gcgcgccgac aatcgggata ttcgacatga tattgccgac
     3241 cggctcctgg aagtaaacgg cgaacagcgg cgcaaagata aacaggcccc acatgccgta
     3301 aacgatactt ggaatggctg ccagcagctc aatggcgata cccagcgggc gtttcagcca
     3361 gcctggcgca agctcagtca ggaacagggc gataccgaaa ctcaccggga cggcgatcag
     3421 cagcgcgata aacgaggtca ccagcgtacc gtagatcggc accagcgccc cgtagatatc
     3481 gttcggtgca tcccactctt tggtccagag gaaagccaga ccaaatttct gaatgctcgg
     3541 ccatgaggag atgatcagag agacaataat gccacccaac atcaatagca caatcagcgc
     3601 cgccagtttt accagcacgc tgaaaattat gtcgcccttt ttacccggtg ggttaaaagc
     3661 aggcttggtt gcagccataa gttactcttc agttaaacgc gtttacaaag tcattttgta
     3721 atgccggata agccgtttaa tgccttatcc ggcctggagt tttattagta cagcggcttg
     3781 ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa cctgttcaac tacgctatcc
     3841 ggcaggctgg cgtaatccag gtcgttcgcc tgtttagccc cggttttgta agcccagtcg
     3901 aagaacttca gcacttctgt gccttgttct ggtttcttct gatctttgtg gatcagaatg
     3961 aacgtggtag aggtaatagg ccacgcatct tcgcctttct ggttggtcag atcctgagcg
     4021 aagtttttgc tccagtctgc gccttttgct gcattagcga agttttcttc ggtcggcaaa
     4081 acaggtttac catcagcgga gatcagtttg gtgtacgcca ggttgttttg tttagcgtaa
     4141 gcatattcaa cgtaaccaat tgcacccggc agacgctgaa cgaacgcggc gataccgtcg
     4201 ttacctttac cgcccagacc gatcggccat tttacggtag agccagtacc aacgttgttt
     4261 ttccactctt cgttcacttt cgccaggtag ctggtgaaga cgaaggaagt cccggagcca
     4321 tctgcgcggc gtactacagc aatgttctgt gaaggcagtt tcagacccgg attcagtttg
     4381 gcgatggctt catcatccca cttcttgatt ttgccgaggt agatgtcgcc gagggtttta
     4441 ccatccagca ccagctcgcc agacttcaac cctggaatgt taaccgccag caccacgccg
     4501 ccaatcacgg tcgggaactg gaacagacct tcctgagcca gtttttcgtc agacagcggc
     4561 gcgtcagaag caccaaaatc aacggtattg gcgataatct gttttacgcc accggaagaa
     4621 ccgataccct ggtaattaac tttattaccg gtttctttct ggtaagtgtc agcccatttg
     4681 gcatacaccg gcgcagggaa ggttgcacct gcacctgtca ggcttgcttc tgcaaacaca
     4741 gagaaagcac tcatcgataa ggtcgcggcg acaacagttg cgacggtggt acgcataact
     4801 ttcataatgt ctcctgggag gattcataaa gcattgtttg ttggctacga gaagcaaaat
     4861 aggacaaaca ggtgacagtt atatgtaagg tttatgacag ttttatgaca gagagataaa
     4921 gtcttcagtc tgatttaaat aagcgtttat attcagttaa ttacaaacat taataacgaa
     4981 gagatgacag aaaaattttc attctgtgac agagaaaaag tagccgaaga tgacggtttg
     5041 tcacatggag ttggcaggat gtttgattaa aaacataaca ggaagaaaaa tgccccgctt
     5101 acgcagggca tccatttatt actcaaccgt taccgatttt gccaggttac gcggctggtc
     5161 aacgtcggtg cctttgatca gcgcgacgtg gtaagccagc agctgcagcg gaacggtgta
     5221 gaagatcggt gcaatcacct cttccacatg cggcatctcg atgatgtgca tgttatcgct
     5281 acctacaaaa ccagcatcct gatcggcgaa gacgtacagt tgaccgccac gcgcacgaac
     5341 ttcttcaatg ttggatttca gtttttccag caattcgttg ttcggcgcaa cgacgataac
     5401 cggcatatcc gcgtcaatca gcgccagcgg gccgtgtttc agctcgccag cggcgtaggc
     5461 ttcagcgtga atgtaagaga tctctttcag cttcaatgcg ccttccagcg cgattgggta
     5521 ctgatcgcca cgtcccagga acagcgcgtg atgtttgtca gagaaatctt ctgccaaggc
     5581 ttcaatgcgt ttgtcctgag acaacatctg ctcaatacgg ttcggcagcg cctgcagacc
     5641 atgcacgata tcatgttcaa tggaggcatc cagacctttc aggcgagaca gtttcgccac
     5701 cagcatcagc agcacggtta actgggtggt gaatgcttta gtggatgcca cgccgatttc
     5761 cgtacccgcg ttggtcatca gcgccagatc ggattcgcgc accagagaag aacccggaac
     5821 gttacagatt gccagcgaac caaggtaacc cagctctttc gacagacgca ggcccgctag
     5881 ggtatccgcg gtttcgccag actgtgacaa ggtgatcatc aggctgttac gacgcacggc
     5941 agatttgcga tagcggaatt cagaggcgat ttcgacgtcg cacggaatac ctgccagcga
     6001 ttcaaaccag tagcgggaaa ccataccgga gttataagaa gtaccacagg cgaggatctg
     6061 aatatgctca accttcgaca gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac
     6121 ctgaccgtgg ctgatgcgcc cggtaagggt gtttttgatc gcgttcggct gttcgtagat
     6181 ctctttctgc atgtagtgac ggtaaatacc tttatcgccc gcgtcatatt gcagattgga
     6241 ttcgatatcc tgacgtttta cttccgcgcc agttttatcg aagatgttta ccgaacggcg
     6301 agtgatttcc gcaatatcgc cctcttcaag gaagataaag cgacgggtca ccggcagcag
     6361 cgccagctgg tcagaagcga taaagttttc acccataccc agaccaatca ccagcggact
     6421 accagaacgt gctgccagca gggtatccgg gtgacgggag tccatgatca ctgtaccgta
     6481 cgcaccacgc agttgcggga tagcacgcag aacagcctca cgcagagtac cgccctgttt
     6541 cagctcccag ttcaccagat gggcaatcac ttcggtgtcg gtttcagaaa cgaaggtata
     6601 gccacgcgct tttaactctt cacgcagcgg ttcatggttt tcgatgatgc cgttatgcac
     6661 caccacaatg tgttcagaaa catgcggatg cgcgttaact tctgaaggtt caccatgggt
     6721 cgcccagcga gtgtgagcaa taccggtgcc gccatgcaga ggatgttctt ccgctgcctg
     6781 agccagcatc tggactttac cgaggcgacg caggcgggtc atatgacctt ccgcatcaac
     6841 aacggccaga ccggcagagt catatccgcg gtattccaga cgacgtaaac cttcaagaag
     6901 gatttctgct acatcacgtt gcgcgatcgc gccaacaatt ccacacatag tttttgattc
     6961 cgatttatat cgttgtcggt caacctgtat gcccgttttt tcgggcgccc cgagccttgt
     7021 agagagtggg gttattttta tagttactgc ttgtgggagg gagattatgt tatctcctca
     7081 tcccatgtga ccggattacc cggccagaat cactttttct ttaccggacg acgccagcct
     7141 tctttctgag tctgcggcac acggctgata gctaatgcat tttcgccgac attacgcgtc
     7201 acagttgtac ccgcagcaat ggttgcacct ttgcctactg ttaccggggc caccagctga
     7261 gtgtcggaac cgacaaacac atcgtcgccg ataatggtct taaatttatt cgcaccatcg
     7321 tagttgcagg taatggttcc cgcgccgatg ttaacgttat cgccaatttc cgcatcgcca
     7381 aggtaagtca gatgaccagc tttcgagcct ttacccagac gtgctttttt catctcaaca
     7441 aagttaccga cgtgtgcacc ttcgagcaac tcagcaccag gacgcaaacg ggcaaacggg
     7501 ccaatggtac aggccgccgc cagattcgcg tcttccacga cggtatatgg gctaatttcg
     7561 caatcatcgc caatcacgct gtttttaatc acgcaaccgg tgccgatttt cacgcgatga
     7621 ccgagagtca cgttgccctc gatgataacg ttagtatcaa tttcaacatc gcgcccgtga
     7681 gtaagcgtac cgcgcagatc aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc
     7741 agtttttcag cctgttcgga ctggtaaacg cgctccagac gggagagttg caggcggtta
     7801 ttcacgcctt ctacttcgct taaacgttgc ggatgaacgg cgacgatttc acgcccttcc
     7861 tgatacgcca gcgcaataat gtcggtgatg tagtattcgc cctgagcatt attgttggta
     7921 agcttcgcca gccagcgttt catatctgcg ccgttagcga tcagaatgcc ggtgttgatc
     7981 tcctgaatct gacgctgctc gtcggtggca tctttgtgct caacaatgcc ggtaactttg
     8041 ccgttttcac gggtgatacg tccataaccg gtcggatcat ccagtttcac cgtcagcaga
     8101 ccaatgccac cctgcggttt agcatcacgc agacgctgga gtgtttcgac agagatcagc
     8161 ggcacgtcgc cgtagagcat taaaatgtct tcatcatcgg caaagaaagg tgcagcctgc
     8221 tgcattgcat gaccagtacc cagctgttct gcctgaagca cccagttcag gttgtcgtct
     8281 ttcagcgcct gttttagcag atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg
     8341 cctaattcat tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc aagggtatgc
     8401 agcactttcg gaagatcgga atacatgcgc gtgcctttac ctgcggcaag gatcactacg
     8461 ctcatagcat tattcaacat acgcgtcctg actgtaattt gagaacgaat ttaaaccgct
     8521 tcaccttgaa aaaactacat ttttttcatc gtgaaatgga cagaggataa attgttcaat
     8581 aacggattat ccccgctgac gaaagcgcca ttttcgacca tcgtcacgcc ttttgtctgc
     8641 agaaaattaa ggcagtacct tcatttaagc gtcaggtgga tgtttttgct cttatttcga
     8701 tcaatgaata aacgggaaaa cagggggttt tatatcagca ggatctatgt gaacgctatt
     8761 caggacgggt cacacgcgca aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt
     8821 ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc
     8881 tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg
     8941 ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat
     9001 tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag
     9061 atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc
     9121 ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg
     9181 gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta
     9241 agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct
     9301 tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc
     9361 atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg
     9421 gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgc
     9481 gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc gatgatgtct
     9541 ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc gtagtgttcc
     9601 tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg
     9661 tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag
     9721 gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc ctgtacggag
     9781 gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat ctcttccgcc
     9841 agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta
     9901 ccggctaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca
     9961 cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt tcccggcggc
    10021 tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc ggtcatttcg
    10081 tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg
    10141 gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt acctacaccc
    10201 gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact
    10261 ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga aggtgctgcg
    10321 cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc
    10381 agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg
    10441 aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc gatggtacgc
    10501 acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc
    10561 acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg
    10621 gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg
    10681 tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac
    10741 gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg
    10801 ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat
    10861 aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt
    10921 cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca
    10981 gcaactggct gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt
    11041 aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca
    11101 gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca
    11161 cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt
    11221 tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac
    11281 cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt
    11341 ccaggtaagg gtgcttgtat tccagattac cgtgtgcaag gtgaccaatc actttgcgca
    11401 tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag
    11461 cggcgaccat ctccatcgct ttagtgatct tctgcgtgtt ctggacgctt gcgatcttac
    11521 tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag
    11581 gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag
    11641 cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc
    11701 gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccgattt tcgacagttc
    11761 aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct gcgcaacgga
    11821 catcggcgca tactgtttct gtttcagcag ttcggtcact ttttgaccgt ggtcaagctg
    11881 cttacgagtt gcatcgtcga ggtcggatgc aaactgagag aacgctgcca gttcacgata
    11941 ctgtgccagc gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc
    12001 accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac cggcgttaaa
    12061 caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa
    12121 cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag aaccggtttt
    12181 ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac gcgcagcacg
    12241 ctccagcaga cgagagtgga ggtagaatac gtcgcccggg aatgcttcac gtcctggcgg
    12301 acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca ggtcatcgta
    12361 aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc aaccagcata
    12421 tggtgccaga tattgcagtg cagcggattc agacgcggtt gctaccacaa cgatggtgtt
    12481 agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg
    12541 gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat
    12601 agccagtgcg gttttaccgg tctgacggtc accgatgatc aattcacgct gaccacgacc
    12661 gattgggatc atggagtcaa cggctttata accggtctgt accggctgat ctacggactg
    12721 acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg
    12781 acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc
    12841 aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc
    12901 gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg cgatagcgta
    12961 acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg
    13021 gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt
    13081 gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct
    13141 ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc
    13201 atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt
    13261 gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag
    13321 ctgttgttca ctcaatgcag cagcggaaat gacgtctacc tcagcggtag cctcactcac
    13381 ggcacgcagg tgaataaact gctccagaac atccgggagc gctttaagac gaccattttc
    13441 agccataacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat
    13501 aaacgactcg gcgagcgttt ctggcgccag tgcgccagag agaagctctg ccatttgttc
    13561 gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg
    13621 ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga
    13681 catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca
    13741 gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact
    13801 tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttccgc ctgcgcctgg
    13861 gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag
    13921 cggcgtttgt ttgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg
    13981 tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg
    14041 ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac
    14101 ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta
    14161 agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag
    14221 caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg
    14281 gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc
    14341 aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc
    14401 accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag
    14461 gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat
    14521 gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca
    14581 gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac tgtgaccacc
    14641 acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca
    14701 gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga
    14761 caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga
    14821 tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt gccatagaca
    14881 gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc agtacatgtt
    14941 cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc
    15001 agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt
    15061 tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac ttacctggca
    15121 caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca
    15181 ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttttgtg
    15241 gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt
    15301 aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac
    15361 agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa
    15421 taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa
    15481 agcttcgcca aatgcgaatg tccaagccac ccggcctttc gctggtgtat gcgcctggtg
    15541 acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc ccccgcttat
    15601 tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac
    15661 cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat
    15721 cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa
    15781 ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa
    15841 caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga
    15901 cttttctact aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat
    15961 aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc
    16021 aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa
    16081 tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta acagccaatg
    16141 atgattctta gcgccgattt ttagcagact gatattttca ctaatgactt attttctgct
    16201 taccaaaaaa agccacgtta tcttgttgat gcaaaa
//
LOCUS       DAZUGK010000100        16204 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZUGK010000100 DAZUGK010000000
VERSION     DAZUGK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.100"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..1108)
                     /locus_tag="ACHY5W_002781"
     CDS             complement(59..1108)
                     /locus_tag="ACHY5W_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4447657.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1207..2457
                     /locus_tag="ACHY5W_002782"
     CDS             1207..2457
                     /locus_tag="ACHY5W_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4447658.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPLEHVPEAASSS"
     gene            complement(2461..3018)
                     /gene="dcrB"
                     /locus_tag="ACHY5W_002783"
     CDS             complement(2461..3018)
                     /gene="dcrB"
                     /locus_tag="ACHY5W_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFS4447659.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSHDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(3091..3756)
                     /locus_tag="ACHY5W_002784"
     CDS             complement(3091..3756)
                     /locus_tag="ACHY5W_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFS4447660.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            3976..4221
                     /gene="tusA"
                     /locus_tag="ACHY5W_002785"
     CDS             3976..4221
                     /gene="tusA"
                     /locus_tag="ACHY5W_002785"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFS4447661.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            4366..4674
                     /locus_tag="ACHY5W_002786"
     CDS             4366..4674
                     /locus_tag="ACHY5W_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447662.1"
                     /translation="MDYLIPVCLGLLGLFAMFAPNWLMPVLIAVGGWLGVLACGWLWL
                     MIFLPLLNGVGLAYALWLMFKRPSFTSRLVATCCVISVILMLALYYTFLTMPPFPAQH
                     "
     gene            complement(4707..6905)
                     /gene="zntA"
                     /locus_tag="ACHY5W_002787"
     CDS             complement(4707..6905)
                     /gene="zntA"
                     /locus_tag="ACHY5W_002787"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFS4447663.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAIHSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASTIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(6979..7605)
                     /locus_tag="ACHY5W_002788"
     CDS             complement(6979..7605)
                     /locus_tag="ACHY5W_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFS4447664.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAITAACYFAGHFLIVRSLYL"
     gene            7746..8105
                     /locus_tag="ACHY5W_002789"
     CDS             7746..8105
                     /locus_tag="ACHY5W_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFS4447665.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(8108..8377)
                     /locus_tag="ACHY5W_002790"
     CDS             complement(8108..8377)
                     /locus_tag="ACHY5W_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFS4447666.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8367..8963)
                     /gene="rsmD"
                     /locus_tag="ACHY5W_002791"
     CDS             complement(8367..8963)
                     /gene="rsmD"
                     /locus_tag="ACHY5W_002791"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFS4447667.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADESLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            9113..10609
                     /gene="ftsY"
                     /locus_tag="ACHY5W_002792"
     CDS             9113..10609
                     /gene="ftsY"
                     /locus_tag="ACHY5W_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4447668.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPEPVVEEMP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10612..11280
                     /gene="ftsE"
                     /locus_tag="ACHY5W_002793"
     CDS             10612..11280
                     /gene="ftsE"
                     /locus_tag="ACHY5W_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4447669.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            11273..12331
                     /gene="ftsX"
                     /locus_tag="ACHY5W_002794"
     CDS             11273..12331
                     /gene="ftsX"
                     /locus_tag="ACHY5W_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4447670.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12576..13430
                     /gene="rpoH"
                     /locus_tag="ACHY5W_002795"
     CDS             12576..13430
                     /gene="rpoH"
                     /locus_tag="ACHY5W_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFS4447671.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13701..14804
                     /gene="livJ"
                     /locus_tag="ACHY5W_002796"
     CDS             13701..14804
                     /gene="livJ"
                     /locus_tag="ACHY5W_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFS4447672.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            14954..15220
                     /gene="ataR"
                     /locus_tag="ACHY5W_002797"
     CDS             14954..15220
                     /gene="ataR"
                     /locus_tag="ACHY5W_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFS4447673.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAVKRLQGM"
     gene            15227..15754
                     /gene="ataT"
                     /locus_tag="ACHY5W_002798"
     CDS             15227..15754
                     /gene="ataT"
                     /locus_tag="ACHY5W_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFS4447674.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDHSI
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(15751..16134)
                     /gene="panM"
                     /locus_tag="ACHY5W_002799"
     CDS             complement(15751..16134)
                     /gene="panM"
                     /locus_tag="ACHY5W_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFS4447675.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         3932 a         4148 c         4416 g         3708 t
ORIGIN      
        1 tcaaatcaaa tggccactgt tagataaacg gtggccattg cctgttcagg aggtgctatt
       61 aacccggtaa ccgacttttc ggtctgcccg gccccagtaa aatcgccagt ttgctgccac
      121 ctttcgtcgt ttccatccag attttacaca cgctggttaa tggcaccgaa agtagcatcc
      181 ctaccgggcc gagcagccat ccccagatta acaatgaaag aaataccacc atggtggaca
      241 tccccaggcg atggcccatc atccgtggtt ctaaaatatt gccgatgacc atatggacca
      301 ctaaaaacaa tgcgccgacc agaatacatt cgtaaacacc attaaacagc agcacctgaa
      361 tcattggcgg tacggcggaa attaccgcgc cgatattggg cacgtagttg agcaaaaacg
      421 ccagtactgc ccacatcagc gcaaactgca cccccatcag ctccagcccc agccagacga
      481 tgacacctgt ccataaactg agtagcgtct ttaacgccag ataatgcgaa acgcctttaa
      541 gtgcgcggtg taatcctgcg atgtgaatct gtgggttatt cagcgcaaaa cgcattttgt
      601 aagggacgtg gcgcacttca aacagcataa aaactacggt catcaccagc aaaagcacgc
      661 tcgccattgc cccggaaagc ccggtcatta gcgctgtggt gaaggtaacc actttttccg
      721 agtccatccg ctgcagcatt cgctccggcg acatatgcaa attaagaaaa ggcaacatct
      781 cctgcaattt aaaaagtttg cgcgtcagct ccttattaaa cttcggcagc atagagataa
      841 attcgttaaa cgatgccgcc agtacgccga ccagcgcggt tagtgcgatc agcatcacca
      901 ccactacaat cgtaatggca acggggcgtt gtactccccg acgaataaac caggtgacga
      961 gcgggttgag gacgatggca aaaaacagcg ccagtaacag ctgcacaatg atatctgccg
     1021 ctgcgtgaat gcccgcgagg atcactacca gcgaggccag cttgagcaga atgtgcatgc
     1081 ccgttttatc gggttgaggg gtttccattg gggcttcctt gtgacttttt gtattaagtg
     1141 tagcgggagt cacgccagca atattctgat tcttactgct gattttcacg tcagcacgtg
     1201 gtaaaaatga aacactgttg taaaaatgtg gtgatcctca tgcccgaacc cgtagccgaa
     1261 cccgcgctaa acggattgcg cctgaatttg cgcattgtct ccattgtcat gtttaacttc
     1321 gccagctacc tcaccatcgg gttgccgctc gctgtattac cgggctatgt ccatgatgtg
     1381 atgggcttta gcgctttctg ggcaggattg gttatcagcc tgcaatattt tgccaccttg
     1441 ctgagccgtc ctcatgccgg acgttacgcc gatttgctgg gccccaaaaa gattgtcgtc
     1501 ttcggtttat gcggctgctt tttgagcggt ctgggatatc tgacggcagg attaaccgcc
     1561 agtctgcctg tcatcagcct gttattactt tgcctggggc gcgtgatcct tgggattggg
     1621 caaagttttg ccggaacggg atcgaccctg tggggcgttg gcgtggttgg ctcgctgcat
     1681 atcgggcggg tgatttcgtg gaacggcatt gtcacttacg gggcgatggc gatgggtgcg
     1741 ccgttaggcg tcgtgtttta tcactggggc ggcttgcagg cgttagcgtt aatcattatg
     1801 ggcgtggcgc tggtggccat tttgttggcg atcccgcgtc cgacggtaaa agccagtaaa
     1861 ggcaaaccgc tgccgtttcg cgcagtgctt gggcgcgtct ggctgtacgg tatggcgctg
     1921 gcactggctt ccgccggatt tggcgtcatc gccaccttta tcacgctgtt ttatgacgct
     1981 aaaggttggg acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg
     2041 cgtttgttat tccctaacgg cattaaccgt atcggcggct taaacgtggc gatgatttgc
     2101 tttagcgttg agataatcgg cctgctactg gttggcgtgg cgactatgcc gtggatggcg
     2161 aaaatcggcg tcttactggc gggggccggg ttttcgctgg tgttcccggc attgggtgta
     2221 gtggcggtaa aagcggttcc gcagcaaaat cagggggcgg cgctggcaac ttacaccgta
     2281 tttatggatt tatcgcttgg cgtgactgga ccactggctg ggctggtgat gagctgggcg
     2341 ggcgtaccgg tgatttatct ggcagcggcg ggactggtcg caatcgcgtt attactgacg
     2401 tggcgattaa aaaaacggcc tctggaacac gtccctgagg ccgcctcatc atcttaaaac
     2461 ttactgaata accagcgtat taatgatgtt ttctgcggtg gtctgcgctt tttgctgatt
     2521 gtcagctggc agagtaattt gcatggtcag cagttgatta cccacgttac ccagaataac
     2581 ggaagagtac gccgtctggc ctttcgcgga gataatactg tctaactgct gcattttgtg
     2641 acctttcagc tcaatggctt tattggttac cacttgcagc tgcggatcgt ggctacgttg
     2701 ctgatcttcc agacgcttcg ccagcaccgc cagatcttct ttcggatcat cgcccatgat
     2761 gacgatgact gctttctgcc cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt
     2821 tcccagctta ccgctctggt cggtcatatc cgctggcagc gagaaactta acttgccatc
     2881 aagcaggttg acgggattcc cggtagcgtt actttccgcg accgaaccct gcgccgtagc
     2941 gttagtgtct ttatcatcac aggccgcaag ccccataacc agcaggccaa ttccgacata
     3001 tttaaccaga ttgcgcattg acttcttcct ttcgataaac ggccataacg gctcattcat
     3061 ccatcttatc acaactctga taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga
     3121 tttatctgcc agtcttttca acagcatatt gagtaatacg ccatacattg gcaggaagaa
     3181 aacgatactg attaacactt tgaaacagta atcgaccagc gcgatttcca tccagtgttc
     3241 agccataaag gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct
     3301 gacgttaccg aacagtgtgg acgctgtcgg tgccagccac cagcggcgac tctgacgcag
     3361 gcggttaaaa acgtgcacgt cgaggatctg ccccagcgcg taggccatga aactggcggt
     3421 ggcgatacgg gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc
     3481 catatagaat agcgacgaga tgacgtagga gatcagcaaa gcggggatca tcaccgcaaa
     3541 gatgatacga cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg tagcaaggaa
     3601 aataaacggg aagctaaacg cgccccaggt ggtatggaaa ccaaaaatgg agacgggaag
     3661 ctgaaccaga tagttactgg aggtgatcac cagcagatga aataacgata accagaacaa
     3721 cgccttatag cgttgagttt gcgagaaaac gttcatattg tacctttttg attaaccatt
     3781 ggggtgaggg aacccaatac gtacgacacg tctgttacct taacataata acgtgcttac
     3841 ccttttttcg agccgccgca tgatactgct ttacgttgac aatgcaatgg ttaattttca
     3901 cgcaatcgtt aacctggttt gcttacggac tcacagggcg taaactagcg ccgtttttta
     3961 tgtgatgaga agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt
     4021 ggcctgcgct gcccggaacc cgtaatgatg gtgcgcaaaa cagtgcgcaa tatgcagcca
     4081 ggtgaaacgt tgctgattat tgctgacgat ccggcgacga cccgcgatat ccctgggttt
     4141 tgtaccttta tggaacacga actggttgcc aaagagacgg atggactgcc ttatcgctat
     4201 ttgattcgta aaggtggtta aggtggatca cggactgtta ccctaaccct ctcccctgag
     4261 gggcgagggg accgatcgca ctcaatgttg ctgcttgaaa ccggtacgta ggacggataa
     4321 ggcgttcacg ccgcatccgg caagcaagtg taatggaata cacaaatgga ttatttaatt
     4381 cctgtttgcc tgggattgct gggattgttc gcgatgttcg cccccaattg gctgatgcct
     4441 gttttaatcg ccgtgggggg atggctgggg gtacttgcct gtggttggct ctggctaatg
     4501 atttttttac ctttacttaa cggcgtcggg cttgcttatg ccctgtggtt gatgttcaaa
     4561 aggccatcat tcacgtcccg cctggttgct acatgttgtg ttatctccgt cattctgatg
     4621 ttagcgctat attacacttt cctgaccatg ccaccgttcc cggcccagca ctaaccctga
     4681 tagccagagg ctatcaggca acacctttat ttcctgcgca acaaccttaa cgcattcgcc
     4741 gttaccagca ccgtcgcacc cgtatctgcc agcaccgcca gccatagtcc ggtcattcct
     4801 aacagcgtgg tgacgaggaa gatccctttc agccccagcg caatagtgat gttctggcgg
     4861 atattggcgt gagtggcgcg tgccagttca atcatttgca ccagaccgcg caggtggtta
     4921 tgggttaatg ctgcgtcggc ggtttccagc gccacgtctg tgccgctgcc cattgcaatc
     4981 ccgatggcgg cagctttcat cgctggcgcg tcgttaatac cgtcaccgac catcgccagc
     5041 ggcgcatgtt gattcagctc ggtcaccgct ttgactttat cttccggcaa caggcccgct
     5101 ttaaactcca gccccagctc cccggcaatt gccgccgctg cgcgtggatt atcgccggtg
     5161 aggatcaccc ctttgacgcc cagcgcgttc agttcactga tggcagttgc agcatcggcg
     5221 cgcagggtat cctgtaacgc aatgacacca agcacgtcat cgttacgtac taccagcact
     5281 accgtttgcc cggcgctttc cagttcgtta attaaaccag caaatgcatc agcgggatgt
     5341 ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct gcgcttcaat gccagacccg
     5401 accagcgccc gctgtgattc ggcggcggga atggcgagtg cagcaacctg tgcttcgcgc
     5461 acgatggcct gcgccagtgg atgcgtcgcg ccttgctcga ccgccgccgc cagtgtcagc
     5521 agttcagatt cactaatacc cgttgccgga tgaatcgcgg taacgcgcgg tttaccgacg
     5581 gtcagcgtac cggttttatc aaacgccacc tgggtaacac gacctagctg ctccagcgcc
     5641 gcaccgcctt taattaacgc cccacgacgt gccgctgctg ccagcccgga ggtaatcgcc
     5701 gcgggcgtgg agataactaa cgcacacggg cagccaatca gcagcagcgt cagcccttta
     5761 taaatccact cttgccagct ggcggcaaac agcagcggcg gcaccagcgt taccagcagg
     5821 gcgacggcca taattgctgg cgtataaata cggctgaaac ggtcgataaa ccgctcaatg
     5881 ggggcacggc gctcttcggc ttcttcgatc agtttcagaa tccggtcaat ggtgctggca
     5941 cccggttctg acagcacttc cagcgttacc agacggtcta cgctggtggc tcctgcagga
     6001 actttatcgc ccgttgcgcg ctccaccgga atggattcgc cggtcagggc gctttcatca
     6061 aaactggcaa agggcgagag cagtttaccg tcggcaggca aacgcccgcc cgccgcaact
     6121 tcaatcacat cgccagggcg caggctgtga atcgccacct cttcccgctc accgttacgc
     6181 aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc taacgccctg acgcgcgcgg
     6241 ctggcggccc agccttccag tcgttcacca atcaaaaaca gcagcaacac catcgcagct
     6301 tcagccgttg cgccaataaa cagcgcacca atagcagcta cgctcattaa ggtttcaatg
     6361 gcgaagtagc tgccggattt gatcagccgt aatgcctgac gagcaatcgg gtacagccca
     6421 accagcgtgg tcgcgataaa cgccagttgc ccgaacggat gattgaactg ctccagaccc
     6481 cagctgattg ccatcatcac gattagcgta atcagcggca gattctcttt caggcgcgat
     6541 gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat agcccgcttt ttgcaccgca
     6601 gattcaacct gggcacggat gtcattgtcg gcatcaacca ccagtttttc ggtggcgaac
     6661 agtacctgca cctgattcac gcctgcaagc tggcgcacgg cattttctac cttgcgcgca
     6721 caggcggcgc agtccatgcc gctgactttc cagctatagc gggtgccgga gacgttttca
     6781 gagagcgttg gcgtgctgga acatgcgccg tcgcagcaac agtcgttggc gttctgtacc
     6841 gtggttagcg gtttgaacgc agcaaattga ggggctttct tgccgtgatt gtcaggagtc
     6901 gacatggcat cctccggtta agttttttct cattaaccga aggatacact ctggagtcga
     6961 ctccagagtc aagttttatc agagatacag cgagcggacg atgaggaagt gaccggcaaa
     7021 gtagcaggcc gcagtaatcg cgttatccgc acggaagcgt cggcgatagt ggctacccag
     7081 ccagacaaag ttactgataa acagcaacga agcgccgaca aacgcagaga gtgccggagc
     7141 ggtcggacgg aagaaccaca gttcacctgc cagccacacc atcaccagcg tcatgccgat
     7201 aaacgtgcag atgggccaac gatactcttc cagccgcgtc cagataatcg ccagtaacag
     7261 cgcgcccaga accagcaaca ccagtggcag aggccagaag aaagagagcg tcatctgact
     7321 ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac gcgccgatgg cgtacatcag
     7381 acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt gaggcgcaca gccccgccag
     7441 caccagatag ctaatggcgt cgaacatcgg cgcttgccag gccagtaaca acaggagaag
     7501 aagggttaac ggtttaaaca cccagcgttg ccaggttggc ccacgatacg atgcatccac
     7561 agatagccat gcggaaagac agacagcgat aaacgaccaa agcatcttag ttccttgaat
     7621 tcgttatttt ttcgtaggcg gaatgcctac ggtcttgctt ccagtttagt ggcgagggtg
     7681 gacggatgac aacaccataa ctggcagggt atgcttaatt ccgcattttc cgatgaggga
     7741 taaagatgag caaaccacct cttttcttta ttgttatcat tggcttaatc atcgtcgccg
     7801 catcgtttcg ttttatgcaa cagcgacggg aaaaagctga taatgatatg gcaccgctcc
     7861 agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc gattaacgat cgccgttcgc
     7921 gtcagcagga agtgactccg gcaggcacca gtatgcgcta tgaggcaagc ttcaaaccgc
     7981 aaagcggagg aatggagcag acgtttcgcc tcgacgccca gcagtaccac gccctgacag
     8041 tgggcgataa aggtacgctg agctataaag gaacgcgctt tgttagcttt gttggcgaac
     8101 aataaggtta ttttttaact ttaaatttct tctgccagac cagcagttca aacacgccga
     8161 aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg gccatctttc ggtaaagtgg
     8221 atttcaatag cgccagctgc ataccatgca tcagcacggt aaaaatcagc gccacgttaa
     8281 cgaaaatatt cagcgggcgc gggaagggat gcaccaggtt gaggattaaa aatccccaaa
     8341 cgcagagcat taacaaacgt ccaatattaa tcagcatcac tttctccttg tgcttcgcgt
     8401 tgatacagcc gataagccac ctgacccgcc actttttccc gatgcaatga ccagtttgcc
     8461 ggaacggtgg gcagaccgtt ttcgacttcg ctttcgacat aaatcaggga ttcgtcagcc
     8521 agccagccgt tatcttccag taaatttatc gtctcttcta acaagccacg gcggaacggt
     8581 ggatcgacaa acacgatatt atgcggtgtg cctttttgcg ccaggaatga catcgcgttg
     8641 ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca gattcttaat taactgctga
     8701 gaaaccgcgc gatccatctc aatcaacgtg gcccccgcag catagcgcga taacgcttcc
     8761 agccccagcg cgccgctccc ggcgaagcaa tccagacatt gggcgtcaac aatgaccgga
     8821 gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg tggggcgcag acctgggcta
     8881 tcaggaaccg gtagtttacg gcctcgccac tgcccgccaa taatgcggat ttggccgctg
     8941 ccggaatgat tcggtttttt catgataaat tgctcaatcc gccagataac acatacttgc
     9001 aggcggtgat gtgaattaag ttaagtgata gactatttca tcattttttt agctgctatg
     9061 tacatagcgt taacgctgtg ccatgaagca acagcgagga gtgtagtcgc aaatggcgaa
     9121 agaaaaaaaa cgtggctttt tttcctggct gggctttggt caaaaagagc agaccccgga
     9181 aaaagagaca gaagttcaga atgaacaacc ggttgtagaa gaaatcgttc aggcgcaaga
     9241 gcctgtgaag gcttctgaac aagccgttga agagcagccg caggcgcata ctgaagccga
     9301 ggcggaaact tttgctgccg aagttgtgga agtcactgaa caggtagctg aaagtgaaaa
     9361 agcgcagcct gaagcggaag tcgttgcaca gccggaaccg gtcgtagaag aaatgccgga
     9421 gccagtggct atcgagcgtg aagagctgcc gttgccggaa gacgtcaacg ccgaagaggt
     9481 ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag attgtcgaag cggcggaaga
     9541 agaagcggct aaagaagaaa ttaccgacga agagctggaa acacaggcgc tggctgccga
     9601 agcggcagaa gaggcggtga tggtggttcc tccggcagaa gaagagcagc cggtggaaga
     9661 aatcgctcag gagcaggaaa aaccgaccaa agaaggtttt ttcgcgcgcc tgaaacgcag
     9721 cctgttaaaa accaaagaaa atctcggttc cggatttatc agcctgttcc gcggtaaaaa
     9781 aatcgacgat gatctgtttg aagagctgga agaacagttg ttgatcgccg atgtgggcgt
     9841 ggaaactaca cgtaaaatta tcaccaatct gacggaaggc gcatcccgca agcagcttcg
     9901 tgatgccgag gcgctctatg gcctgctgaa agaagagatg ggcgagattc tggcgaaagt
     9961 cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg atcctgatgg tgggcgtcaa
    10021 cggtgtgggt aaaaccacga cgattggtaa gctggcacgt cagtttgagc agcagggtaa
    10081 atcggtgatg ctggcggcgg gcgatacttt ccgtgcagca gcggttgaac agcttcaggt
    10141 ctggggtcag cgcaacaata ttccggtgat tgcccagcat accggtgcgg attccgcctc
    10201 tgttatcttc gacgccattc aggcagctaa agcgcgtaat atcgacgtcc tgattgccga
    10261 taccgccgga cgtctgcaga acaaatcgca tctgatggaa gagttgaaga aaatcgtccg
    10321 cgtgatgaag aaactcgacg ttgaagcgcc gcatgaagtt atgctgacta ttgatgccag
    10381 caccgggcag aacgcggtaa gccaggccaa actgttccac gaagctgttg gcttaaccgg
    10441 catcacgcta acgaaactgg acggcacggc gaaaggcggg gtaattttct cggtggctga
    10501 ccagtttggt atccctatcc gctacattgg tgtcggcgaa cgtattgagg atttgcgtcc
    10561 gtttaaggcg gacgacttta tagaggcact ttttgcccga gaggattaac aatgattcgc
    10621 tttgaacatg tcagcaaggc ttatctcggt gggagacagg cgctgcaggg cgttacgttc
    10681 catatgcagc cgggtgagat ggcgtttctg accggtcatt ccggcgcagg gaaaagtacc
    10741 ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg ggaaaatctg gtttagcggc
    10801 catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc tgcgccgcca gattggcatg
    10861 attttccagg atcaccatct actgatggac cgtactgtct acgataacgt ggcgatcccg
    10921 ctgattatcg ccggtgccag cggtgacgat attcgtcgcc gggtgtcggc ggcactggat
    10981 aaagtcgggc tactggacaa agcgaagaac ttccctattc agctttcggg cggtgaacaa
    11041 cagcgtgttg gcattgcccg cgcggtggtg aataagcccg cggtactgct ggcggacgaa
    11101 ccgactggta acctggacga cgcgttgtcg gaagggattc tacgtctgtt tgaagagttt
    11161 aaccgcgttg gggtaaccgt attgatggca acgcacgaca tcaacctgat ttcacggcgt
    11221 tcctatcgca tgctcaccct gagcgatggt cacttgcatg gaggcgtggg ccatgaataa
    11281 gcgcgatgca atcaatcata ttcggcagtt tggcgggcgt cttgatcgct tccgtaaatc
    11341 ggtcggcggc tcaggcgacg gcggtcgtaa cgcaccgaaa cgcgcgaaat cctcgccaaa
    11401 accggtaaat cgcaaaacca acgttttcaa cgaacaggtg cgctatgcct tccacggcgc
    11461 attgcaggat ctgaaaagca aaccgttcgc cacgttttta acggtgatgg ttatcgccat
    11521 ctccctgacg ctgcccagtg tctgttatat ggtgtacaaa aacgttaacc aggcggcgac
    11581 gcagtattat ccgtcaccgc aaatcactgt ttatctgcaa aaaacgctgg acgatgacgc
    11641 cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc gtggagaaag tgaactatct
    11701 ttctcgtgaa gacgcactgg gcgagttccg taactggtct ggttttggtg gtgcgctgga
    11761 tatgctggaa gaaaacccgc ttccggcagt ggcggtggtg atcccgaaac tcgatttcca
    11821 gggtacggaa tcactcaata cgctgcgtga tcgtatcacg cagattaacg gcattgacga
    11881 agtgcggatg gatgacagct ggtttgcccg tctggcggcg ttgaccgggc tggtcgggcg
    11941 cgtttcggcg atgatcggcg tgttgatggt ggcggcagtg ttcctcgtca tcggtaacag
    12001 tgtccgtctg agtatctttg ctcgtcgtga ctccattaac gtgcagaaac tgattggtgc
    12061 aacggatggt ttcatcctgc gcccgttcct gtacggtggc gcattgctgg ggttttctgg
    12121 cgcattgttg tcactaattt tgtcagaaat tctggtactg cgattgtcat cggcggttgc
    12181 ggaagtggca caggttttcg gaacgaagtt tgatatcaat ggcttatcgt tcgatgaatg
    12241 cctgctattg ctgctggtat gctcgatgat tggctgggtg gcagcgtggc ttgccactgt
    12301 acaacattta cgccacttta cgcctgaata ataaaagcgt gttatactct ttccctgcaa
    12361 tgggttccgt agcagggaga gagaccccgt tgtctcttcc cggtatttca tctctatgtc
    12421 acattttgtg cgtaatttat tcacaagctt gcattgaact tgtggataaa atcacggtct
    12481 gataaaacag tgaatgataa cctcgttgct cttaagctct ggcacagttg ttgctaccac
    12541 tgaagcgcca gaagatatcg attgagagga tttgaatgac tgacaaaatg caaagtttag
    12601 ctttagcccc agttggcaac ctggattcct acatccgggc agctaacgcg tggccgatgt
    12661 tgtcggctga cgaggagcgg gcgctggctg aaaagctgca ttaccatggc gatctggaag
    12721 cagctaaaac gctgatcctg tctcacctgc ggtttgttgt tcatattgct cgtaattatg
    12781 cgggctatgg cctgccacag gcggatttga ttcaggaagg taacatcggc ctgatgaaag
    12841 cagtgcgccg tttcaacccg gaagtgggtg tgcgcctggt ctccttcgcc gttcactgga
    12901 tcaaagcaga gatccacgaa tacgttctgc gtaactggcg tatcgtcaaa gttgcgacca
    12961 ccaaagcgca gcgcaaactg ttcttcaacc tgcgtaaaac caagcagcgt ctgggctggt
    13021 ttaaccagga tgaagtcgaa atggtggccc gtgaactggg cgtaaccagc aaagacgtac
    13081 gtgagatgga atcacgtatg gcggcacagg acatgacctt tgacctgtct tccgacgacg
    13141 attccgatag ccaaccgatg gcaccggtgc tctatctgca ggataaatca tctaactttg
    13201 ccgacggcat tgaagatgat aactgggaag agcaggcagc aaaccgtctg accgacgcga
    13261 tgcaggggct ggacgaacgc agccaggaca tcatccgcgc gcgctggctg gacgaagaca
    13321 acaagtccac gttgcaggaa ctggctgacc gttacggtgt ttccgctgaa cgtgtacgcc
    13381 agctggaaaa gaacgcgatg aaaaaactgc gcgctgctat agaagcgtaa tttccgctat
    13441 aaagcagaga accctggatg cgagtccggg gtttttgttt tttgggcctc tacaataatc
    13501 aatttcccct ccggcaaaac gccaatcccc acgcagattg ttaataaact gtcaaaatag
    13561 ctattccaat atcataaaaa tcgggtatgt tttagcagag tatgctgcta aagcacgggt
    13621 agtcatgcat aaaacgaaat aaagtgctga gcaacaacat cacaacacac gtaataacca
    13681 gaagaatggg gattctcagg atgaacataa agggtaaagc gttactggca ggatgtatcg
    13741 cgctggcatt cagcaatatg gctctggcag aagatattaa agtcgcggtc gtgggcgcaa
    13801 tgtccggtcc ggttgcgcag tacggtgacc aggagtttac cggcgcagag caggcggttg
    13861 cggatatcaa cgctaaaggc ggcattaaag gcaacaaact gcaaatcgta aaatatgacg
    13921 atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac gacggcatta
    13981 aatatgtgat tggtcacctc tgttcttcat caacgcagcc tgcgtctgac atctacgaag
    14041 acgaaggcat tttaatgatc accccagcgg caaccgcgcc ggagctgacc gcccgtggct
    14101 atcagctgat cctgcgcacc accggcctgg actccgacca ggggccgacg gcggcgaaat
    14161 atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa cagcaatacg
    14221 gcgaaggtct ggcgcgtgcg gtgcaggacg gcctgaagaa aggcaatgct aacgtggtgt
    14281 tctttgatgg catcaccgcc ggggaaaaag atttctcaac gctggtggcg cgtctgaaaa
    14341 aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg caaatcctgc
    14401 gtcaggcacg cgcggcaggg ttgaaaactc agtttatggg gccggaaggc gtggctaacg
    14461 tctcgctgtc taacattgcg ggcgagtcag cggaagggct gctggtgacc aagccgaaga
    14521 actacgatca ggttccggca aacaaaccca ttgttgatgc gatcaaagcg aaaaaacagg
    14581 acccaagcgg cgcattcgtt tggaccacct acgccgcgct gcaatctttg caggcgggcc
    14641 tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct gaaagcgaac tccgtggata
    14701 ccgtaatggg accgctgacc tgggatgaga aaggcgatct gaaaggcttt gagttcggtg
    14761 tgtttgactg gcacgccaac ggcacggcga ccgatgcgaa gtaacggata gataaatgaa
    14821 aatgcccgat gcgcttaacg tccatcgggt ttttttatgt ttgtcgaaat ggttgtagaa
    14881 agattaaaca atccaggata tgcttattgt acggtgatgt gccgtacata ttatacggag
    14941 caggagagtt cacatgtctg ctgttaaaaa gcagcgtatc gatctgcgtt taactgacga
    15001 tgacaaaagt atgattgagg aagcggcagc gatatctaat cagtccgtca gccaatttat
    15061 gttgaacagc gcttcgcagc gggctgcgga agtgattgaa cagcatcggc gggtgatcct
    15121 caatgaggaa tcctggacgc gggtgatgga tgcgctgagt aatccaccgt caccaggtga
    15181 aaagctaaaa cgtgcggtaa aacgtcttca gggaatgtaa taatttatgg atgatctgac
    15241 gatagagatt ctgaccgatg atgcagatta tgatctacag cgattcgact gcggcgagga
    15301 agcgttaaat ctctttctga cgacacatct cgttcgtcaa catcgcaaca aaattctgcg
    15361 agcgtatatc ctttgtcgca acactccaga acgtcaggtg ctgggatatt acacattatg
    15421 cggcagttgt tttgaacgag ccgcattgcc ctcgaaatcg aaacagaaaa aaattcccta
    15481 caaaaatatt cccagcgtca ctcttgggcg tctggcaatt gatcattcaa tacaggggca
    15541 gggatgggga gcaacactgg ttgctcatgc catgaaagtc gtctggtcag cctctttagc
    15601 agtaggtatt cacggtcttt ttgtagaggc gctgaatgaa aaagcccata cgttttataa
    15661 atcgctgggc tttatcccgt tggtcgggga aaacgaaaat gcgttatttt tcccaaccaa
    15721 atccattgaa ctgcttttta cacagagcga ttaacgcttc tcccagccgc cctgctgtgc
    15781 cgtaaacccc agcgcctgca taaacgccgt catcacaccg cgatcttcca cgcctgcatc
    15841 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    15901 ccccacaccg cgacgtcgag tgacttcccg cacgcgcagg gaatccagtg ctccctcggt
    15961 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    16021 gcggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    16081 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatggggaa
    16141 cccgtgtaaa tcacaaaagt ggaaccagtg taacgaaata atttaatcgg aggctttctc
    16201 tttt
//
LOCUS       DAZUGK010000101        15943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZUGK010000101 DAZUGK010000000
VERSION     DAZUGK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.101"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            46..2227
                     /gene="sinH"
                     /locus_tag="ACHY5W_002800"
                     /pseudo
     CDS             46..2227
                     /gene="sinH"
                     /locus_tag="ACHY5W_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
     gene            2283..3248
                     /locus_tag="ACHY5W_002801"
     CDS             2283..3248
                     /locus_tag="ACHY5W_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFS4447676.1"
                     /translation="MKHYLKRPMTKVALALALAGYCAVPAAMAEGQGNLRAGQWQSET
                     ETTGTIQGTVPWITRSATEIIDADKSHVSITVDRGGRSASTDGEKQFHIGDKLTANWA
                     IGDTQGDLDDNNTATKATLQWMSYSDQAGGDPKEIGTTGSDTYTIAAADADRYIGLKI
                     TPTTTTGDPNVAEQLILLDLSTNAGGGSDSDDIPEGPVFDDAVKVVIHEQGVNTNLLG
                     KETKLKTNTTYQVMLWKDKNSNGSYDTGEEVTSQYNYRWRFTGTSLQLRTNGGIVNPS
                     YNNSDLVIPVTNAEAKTAFDYSEGGLTLGADGVQGYGLSIDYQRK"
     gene            3543..9962
                     /locus_tag="ACHY5W_002802"
     CDS             3543..9962
                     /locus_tag="ACHY5W_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFS4447677.1"
                     /translation="MDGTLKLIKAITAVFLLFLSFQVGAVLKGGQWQQTGISTDAING
                     TVPGAESASVPVYQGSVQLDPSKTYDIQASVKPIAFSVDDTAARMILVNPHDPEGDLF
                     SNPPVLGWQNQTPPAVSLVWADAATPDTPLSPQPRPDRSFCAQNLAGRSLVAWPRINE
                     QEALPLLYLYTLTGVPNQGTVPLAEHKVTLNIAPAQGDLVTISASGYDDTLQAAKTTV
                     DGSITLTVTPQDCEGNIAGNIPFVIKRKDAENRQGVVNNTSPVNLDTTELTTTATEYR
                     GTTNADGAATVTVTQPNGPGVKTPLVVSLSGISQTSETAVIFTVLTSPDAPQANMWGH
                     MAETLTAMNYTFSRPKLAAEVDNEDGSVNDHGETWSTFTWSGADNHCDILPGMRQFGA
                     LATVIPNSIQETAGWPMQGDYYWSSLAGSTGQHHAADVSNRSEAQKPDSTKYIVSCVD
                     KAEPDVEPELVLTPSNFDESINAAKAEVGDSTTMRLTITDKKNNNQPLAYYYFSLHLD
                     DGVNRKNQTDPAWEAHPVQISGGTNLQKVDDHNYEGMTDVNGQATLQLTQPEGAGVKT
                     HITAKMRSNFNAEDAKDVIFTVVTSPDSEYARMWGHMHGIIDAGSLYKRPLLAAETTH
                     EVGTVRENNEDWALFDQNTSMQAECGTGHIPREISLESLYSAYPGNIIGTEYGWPTAV
                     KDYLTAAEQTTHSSVNLGTGNVDSYSGFKQNYLSCSGNEMVAKVEVTTDRDTTSGSLA
                     RAKVGEQITMTVRTVNALNGERVPFTAFTITKDISLDREESATGFTDPSNGAITLNGT
                     QYGTSQSSMVYSGITDAQGQATVVVEQPQGVGLRTRLVVTPPNSVLPNTINYYVIFTV
                     PTSPDVMGARMWGHMDDTITVDSTTFNRPKLLLEVSNGKDSREENNEKWVIVAQADIE
                     NTSAGGCETRKVPRQTQLTALYNANSNNRILTNHGWPTVFKEYWSSTPIDKVPHLASV
                     WLNNGNVQNTGNDRVYMSCLKDANPPASTITLEVVDQAQWNSTLNAAKLKKGETLQVK
                     VTVKDTAGNPMPGMPFTLKRGDGYTRNNERHVAGSDNIVSPVVVDAGMSDEASLNDSA
                     TAYAALTDADGSKILTITRPDTHGTKTALTAALYSDPTINASMDTIFTVPTSPDTDKA
                     KMWGHMPETLTAGELTFKRPLLFAELAVTNKRKSQDEDNETWALFTLAQAGDASKNGC
                     GAEYIPTQDALVALSSSWQGHAIDGWPVQKTYDSSTPDKNSINDRKYMSVDISKGTSK
                     SLASADLGYLTCQTTANPPVNSIELTSTQTAKYDNVDAVKVRTDYSDTGDTITLTVTT
                     KDAAGNPMGYVPFTLNHGTTVPRTKSTANTQKITNQTKLIYVRDSYNNSNGYFNDGQK
                     FYSMTGPDGTLTFTIYQNYQGIGGRIPLTASIDDGSGRTSTELSAIFTTITSPDTPLA
                     NFWGHMPETFTSSAGVKFRRPQLKAELSGTASFNINNEDWARPDATNKANSSISGCTS
                     PYQPFSSELMGLYNDHPNGALETDLGLPLNAGNYWWAYDQTVSSSTWYNQVLNLMDGA
                     IRNESTTSTAGNLMLCLVDPHTEATKIELTSTLLNADKTGINDNRPTAWAKKGESIPL
                     TIKVTDDAGRPAAYESVTLSRATAQVRTTSDFKSSTADDLLLQFLTPVAQANYKFADD
                     NASWNGVTDGNGIITLTATQDNTWGLVTPFTATLARKTAVKSDLDLMYTVVTSPDTPK
                     ANFWGHMPETVSSADGTQFERMKLWAEMGATSGGGYSLTKVNINNESWPAPYITQRYL
                     PELTPCETARYPSLDELQSLYNRYPNGSIVNEIGWPVKQNSYVWWTNADCPAGANSCE
                     SFDFNTGQIVTVSKSRAFQACLVHPHSTVSTVTLTSTAFDSTAQAAKVKKGEAMPVTV
                     TVKDSAGKPVPNVAFTLRRGDASPRNSGATLYGDVAAMDDITIQPSSGAATLLAVSGN
                     EISGVTGADGTANFTVRQNNTPGYKTPLTVTLDKYSTVTAMMDTIFTVLTSPNVSSAH
                     FWGHMADTAVVNGKTLHRPILTTELPSGANPVSSPIINYENWASAHITDASKWDISKQ
                     CGNIENAPTYNELETLYAVFNSLGWPASPSFPYLSSQRCGMDESTGTEDCSITLANKP
                     GLVTCFQ"
     gene            complement(10031..11407)
                     /gene="xseA"
                     /locus_tag="ACHY5W_002803"
     CDS             complement(10031..11407)
                     /gene="xseA"
                     /locus_tag="ACHY5W_002803"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4447678.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSTTDGTVLKQVKQVKAGDSLTTRLGDGVVISEVSAVTKNRK
                     PRKKAANT"
     gene            11569..13035
                     /gene="guaB"
                     /locus_tag="ACHY5W_002804"
     CDS             11569..13035
                     /gene="guaB"
                     /locus_tag="ACHY5W_002804"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4447679.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13104..14681
                     /gene="guaA"
                     /locus_tag="ACHY5W_002805"
     CDS             13104..14681
                     /gene="guaA"
                     /locus_tag="ACHY5W_002805"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4447680.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRILGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(14774..15300)
                     /locus_tag="ACHY5W_002806"
                     /pseudo
     CDS             complement(14774..15300)
                     /locus_tag="ACHY5W_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
     gene            complement(15316..15834)
                     /locus_tag="ACHY5W_002807"
     CDS             complement(15316..15834)
                     /locus_tag="ACHY5W_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS4447681.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4078 a         3954 c         4189 g         3722 t
ORIGIN      
        1 caatattaga cattatttca gaattaaatt ttcacaggga aaaacatgct gcgatggatt
       61 cgctatataa cgctaatcgt tatttctggt gctgcttatg cggcaccgga gataaatatt
      121 aagcaaagcg aatcttcatt acctgatctg gcaagcgaaa cggctcaaca agaggaacaa
      181 agtaacaaag gcaaatcttt caaggaacgt ggtgccaatt acgttaccga gtccgcccag
      241 cagggctttg agaacctgac tccagaagct cttgaatccc aggcgagaag ctatctgcaa
      301 gggcaaatca catcatctgc acagtcttat attgaaggtg cgctttctcc ttatggtaaa
      361 gttcgctcta acctttccat cggtcagggt ggcgatctgg acggcagttc gatcgactat
      421 tttgttccct ggtatgacaa tcagagcaca gtctatttca gtcagttttc tgcgcaacga
      481 aaagaagaac gcacgatagg taatataggt ttaggtgtaa gacataattt tgataaatgg
      541 cttctggggg gggaatatat tttatgatta tgatttcacc cgcggacatc gccgtttagg
      601 cttaggaaca gaagcctgga cggattattt aaaattttct ggtaactatt atcatccgct
      661 ttcagagtgg aaagattcaa aagattttga tttctattta gaacgacccg cacgtggctg
      721 ggatgtccgg gcggaagcct ggctaccttc ttatcctcag ctggggagca aaattgtttt
      781 cgagcaatat tatggtgacg aagtggcgct catcggcacc gataatttag aaaaagatcc
      841 ctatgcagtg acgcttggcg tgaactatca acccgttccg ttactgacgg tcgggacgga
      901 ttataaagca ggtaccggag ataacagtga tcttagcgtt aacgccacga ttaactatca
      961 gttcggtgtt ccgctaaaag atcaactgaa tagcgataac gtcaaagcag ctcactcgct
     1021 gatgggcagc cgccatgact ttgttgagcg caataatttt attgttctgg aatataaaga
     1081 aaaagatcct ctcgacgtta cgttgtggct gaaggctgac gccaccaatg aacacccgga
     1141 ttgtgtcatc aaggacaccc ccgaagctgc ggtagggctg gaaaaatgta agtggacaat
     1201 caatgcgcta atcaatcatc actataagat tgtcgtcgcc tcttggcagg cgaaaaacaa
     1261 cgctgcgcgt actctggtga tgccggttat caaagaaaat accctcacgg aagggaacaa
     1321 caaccactgg aatctggtgc tgccagcctg gcagtacagc tctgatcccg ccgaacagga
     1381 aaaactcaat acctggcgcg tgcgtctggc tctggaagat gaaaaaggca accggcaaaa
     1441 ctccggcgtg gtggaaatca ccgttcagca ggatcgcaaa attgaactga tcgtcaacaa
     1501 cattgcggat gtgccggatg aaaacaatca cagtcatgaa gccagcgctc aggcggatgg
     1561 cgtcgatggc gtggtgatgg atctcgatgt taccgatagc tttggtgatg acactgaccg
     1621 taacggtaat gcgctgccgg aagatggcct gagtccgcag ctttacgatg ctcaggataa
     1681 aaaagtcacc ctcaccaata aaccctgctc gaccgataac ccgtgcgtct ttatcgcgaa
     1741 gcaagataaa gagaaaggaa ccgtcaccct ctccagtact ttgcccggca ccttccgctg
     1801 gaaagcaaag gccgcaccgt atgacgatag taactatgtc gatgtgactt tcctcggcgc
     1861 ggggggcaat gatgttaatg cctttatcta ccgtgtatcc gcgactaacc ccatcaatct
     1921 tatcggcaat gagaaagagc atttgcctgt tgataacgcc tatcgttttg tcctgtggcg
     1981 cgatgtcaac aaagatggcg ttttccagca gtcggaaaaa ctgacggaag aagaaatggc
     2041 gcaatacgac taccagtggg aatttaccgg acacagcgtc aatggctaca ccggtgcgca
     2101 atcgaatacc agtaatgaag atattgtcat ccctgccact aacgaggaag cggtacagaa
     2161 atttggtgcc caaaaagagg atggcgtgca ggggtatgga ttacgtgtgg tttacagcaa
     2221 gcactagatc ggcgtaatgg ccgatcggcc agtcgctgga aattaatcaa ggagagagtt
     2281 gaatgaaaca ctatttaaag cgaccgatga ctaaagtcgc gctggcgctg gctttagcgg
     2341 gatactgcgc agtgcctgct gctatggcag aagggcaggg caaccttagg gcgggtcagt
     2401 ggcaatcaga gacagaaact accggaacga ttcagggtac agtgccgtgg atcactcgta
     2461 gcgcgacgga aatcatcgat gctgataaat ctcatgtgtc cattaccgtt gatcgtggcg
     2521 gacgttctgc ttcaaccgat ggcgagaagc aatttcatat tggtgacaag ttaactgcaa
     2581 actgggccat tggcgatacc cagggcgacc ttgacgacaa caacactgca accaaagcga
     2641 cgctgcaatg gatgagctat agcgaccagg cgggggggga tccgaaagag atcggtacaa
     2701 cgggcagtga cacctatacc attgccgcag cggatgccga tcgctacatc ggtttgaaaa
     2761 ttaccccgac caccacgacc ggtgatccta acgttgcaga acaactgatc ctgttagacc
     2821 tctccaccaa cgcgggcggt ggttctgaca gcgatgatat tccggaaggt ccggtatttg
     2881 acgatgcggt taaagttgtc attcatgagc agggtgttaa caccaacctg ctgggcaaag
     2941 aaaccaaact caaaaccaat accacttatc aggtcatgct gtggaaagat aaaaacagca
     3001 acggctccta cgacactggc gaagaggtta ccagccagta caactaccgc tggcgcttta
     3061 ccggtaccag tctgcaactg cgtaccaacg gcgggattgt caacccgagc tacaacaaca
     3121 gcgacctggt tatcccagtt accaacgccg aagcgaaaac tgcgttcgac tacagcgaag
     3181 gtggcctcac ccttggcgcg gatggtgttc agggctacgg tttgtccatc gattaccaga
     3241 gaaaataatc tttaccagca ccattcccgc aagggaatgg tcagattgat gacaaaggca
     3301 aaactgcctg atgtgcttcg cttatcaggc ctacatggtt cctgcaatgt attgaatttg
     3361 cacgattttg taggtcggat aaggcgttaa cgccgcatcc ggcatgaata acgcgcactt
     3421 tgtcaacaat ctggtcattt cagcaaggga atgaatggtg cttctccgca cactttgctt
     3481 agccagcgtt aagcaaagtg ggttgaagtg aaaaaataac tttaaacggg caggtttcaa
     3541 tcatggacgg gactttgaaa ctgataaaag cgataacggc ggtttttctg ctcttcctct
     3601 cttttcaggt tggggcagta ttaaaaggtg gccagtggca acaaactggc atctcgaccg
     3661 acgcaatcaa tggcacagtg cccggggcag agagcgccag cgtacccgtt tatcagggaa
     3721 gcgttcagct tgatccgagc aaaacctacg acattcaggc atctgtaaaa cccatcgcat
     3781 tcagtgttga tgacacagcg gcgagaatga ttctggttaa ccctcacgat cctgaaggcg
     3841 atcttttttc taatcccccc gtgctaggct ggcaaaacca gacgcctcct gctgtatctc
     3901 ttgtgtgggc ggatgccgcg acgccagata cgccgctcag cccacaaccg cgcccggacc
     3961 gctctttctg cgcgcagaat ctggcagggc gttctttggt ggcgtggccg cgaatcaatg
     4021 agcaggaagc gctaccttta ctctacctct acacgttaac tggtgtgcct aatcagggga
     4081 cggtgccgtt ggctgaacat aaagtgacgc tgaatattgc tcccgcccag ggcgacctgg
     4141 tgacaattag cgccagtggc tatgacgata cgttacaagc ggcaaagact actgtcgatg
     4201 gttcaatcac cttaaccgta accccccaag attgtgaagg taatattgcg ggcaatatcc
     4261 cttttgttat caaacgaaaa gatgctgaaa accgccaggg agtggtgaat aacacctcac
     4321 ctgtcaatct tgataccact gaactcacca ccacagcaac ggagtatcgg ggcacgacta
     4381 atgctgatgg cgcagctacc gtcacggtga cgcagcccaa tggtccaggt gtcaaaacgc
     4441 cgctggtggt cagcctttct ggcatctcgc aaaccagtga aacggcggtg atatttactg
     4501 tactcaccag cccggatgcc ccgcaggcca atatgtgggg ccatatggcg gaaaccctga
     4561 cggccatgaa ttacaccttt agtcgtccga aattggcggc ggaagttgat aacgaagatg
     4621 gcagcgtgaa tgatcacggt gaaacctggt cgaccttcac ctggagtggc gcagataacc
     4681 actgcgatat tttgcctggc atgcgccagt ttggcgcgct ggctacggtg atccctaact
     4741 ctattcagga gaccgccggt tggccgatgc agggtgatta ttactggtca tctctggcag
     4801 gaagtaccgg gcagcaccac gcggctgatg tgtctaaccg cagcgaagcg caaaaaccag
     4861 acagtacaaa gtatatcgtg agctgtgtgg ataaagcaga acccgatgtt gagccagagt
     4921 tggtgctcac gcccagtaac tttgatgaaa gtataaatgc cgcgaaggca gaggtcggtg
     4981 acagcactac gatgcgcctg accattaccg ataaaaaaaa taacaatcag ccgctggctt
     5041 attactactt ctcgctgcat ctcgatgacg gcgttaaccg caaaaatcaa accgaccctg
     5101 cgtgggaagc gcatccggta caaatttccg gcggcaccaa cctgcaaaag gtagatgatc
     5161 ataattatga gggaatgacc gacgtcaatg gtcaggcaac tctgcagttg acacagcctg
     5221 aaggcgcagg tgttaaaacg catattacgg cgaaaatgcg cagtaatttc aatgcggaag
     5281 atgccaaaga tgtgatcttt acggtggtga ccagccccga cagcgaatat gcgcggatgt
     5341 ggggtcatat gcacggcatt attgacgctg gcagtttata taaacgaccg ctgctggcgg
     5401 ctgaaaccac gcatgaagta ggcacagtac gggaaaataa cgaagactgg gcgttattcg
     5461 atcaaaatac cagtatgcag gcagaatgcg gcacggggca tataccgcgt gagatctcac
     5521 tggaaagtct gtattcggct taccctggca atatcatcgg gactgagtat ggctggccaa
     5581 ccgcagtaaa ggactattta actgccgcgg agcagacaac gcattcatcg gtaaacctcg
     5641 gaacagggaa cgtcgacagt tatagcggtt ttaaacaaaa ctatctctcg tgctctggta
     5701 atgagatggt ggcgaaggtt gaagtgacaa ccgatcgtga tactacctcg ggcagcctgg
     5761 cacgggcgaa agtcggtgaa caaatcacca tgacggtgcg cacagttaat gctctgaatg
     5821 gcgagcgggt gccgttcacg gcttttacta ttaccaaaga tatcagtctt gaccgtgaag
     5881 aaagcgcaac cgggtttacc gatcccagta acggcgcaat aaccctgaat gggacgcagt
     5941 acggtacctc acaatcctca atggtttact ccggtattac agacgcacag gggcaggcaa
     6001 ccgttgttgt tgagcagcca caaggcgttg gcttaagaac gcgacttgtt gtcacgcctc
     6061 ctaattcagt tttgccgaac actattaact attatgtgat ctttaccgtt cccaccagcc
     6121 cggatgtgat gggagcgaga atgtgggggc acatggacga cacgataacc gttgactcca
     6181 cgacctttaa tcgcccgaaa ttattgttgg aagtgagtaa tggcaaagac tcgcgtgagg
     6241 aaaataacga aaaatgggtg atcgtcgcac aggccgatat cgaaaacacg tcggcgggcg
     6301 ggtgtgaaac ccggaaagta ccgcgtcaaa cacaactgac tgcgttgtac aatgcgaaca
     6361 gtaataacag gatcctaacc aatcacggct ggcctacggt atttaaagag tactggagca
     6421 gcacgccgat tgataaagtc ccgcaccttg cctcagtctg gctgaataat ggcaatgttc
     6481 agaatacggg caatgatcgg gtatatatga gttgcctgaa ggacgccaac ccacctgcca
     6541 gcaccattac gctggaagtc gtggatcagg cacagtggaa tagcacgctt aatgcggcaa
     6601 aactgaaaaa aggcgaaacg ttacaagtga aagtcacggt gaaagataca gcgggcaacc
     6661 ctatgccggg aatgccgttt accctgaaac gtggcgatgg ctatacccga aataatgaaa
     6721 gacatgtcgc aggtagtgac aatattgtct cgccagtagt ggttgatgca ggaatgtccg
     6781 atgaagcgtc actgaatgac tctgccaccg catacgccgc gttaaccgac gctgatggca
     6841 gcaaaattct gaccatcact cgtccggata cccatggcac caaaacagcg ctcactgcag
     6901 cgctctattc tgatccaacg ataaatgcgt cgatggacac catttttact gtacccacca
     6961 gcccggatac tgacaaggcg aagatgtggg gtcacatgcc ggaaacactg actgcgggcg
     7021 agctgacctt taaacgtcca ttactgtttg ccgagcttgc ggtgaccaac aagcgtaagt
     7081 cccaggacga ggataacgaa acgtgggcac tgtttacctt ggcacaagct ggtgatgcca
     7141 gtaagaatgg ctgcggtgcg gaatacatcc ctactcagga tgcgctggtc gccctttcca
     7201 gcagttggca gggccatgcc attgatggct ggccagtgca aaaaacatac gactccagca
     7261 caccggataa aaacagtatc aacgatcgta aatatatgtc cgtggatatt agcaaaggca
     7321 ccagcaaaag ccttgcatcg gcagacctgg ggtatttaac ctgtcagact accgcgaatc
     7381 cgccggtgaa ctcgattgag ctgacatcaa cgcaaaccgc gaaatatgac aatgttgatg
     7441 cggtgaaagt acgaaccgat tacagcgata ccggagatac catcaccctg acggtgacca
     7501 cgaaagatgc tgcgggcaat ccgatggggt atgtgccgtt tacgttaaac cacggcacca
     7561 cggtaccgcg tacgaagagt acggctaata cacagaagat caccaatcag acaaaactca
     7621 tttatgttcg ggatagctac aataactcca acggttattt taatgatggt cagaagttct
     7681 acagcatgac cgggcctgac ggcacgttaa cgtttaccat ttatcaaaat tatcagggga
     7741 taggtggacg tattccacta acggcgagca ttgatgacgg tagtggaaga acatcaactg
     7801 agttgagtgc gatttttacc accattacca gcccggatac gccgttagct aatttctggg
     7861 gccatatgcc ggaaaccttt accagtagcg cgggcgtcaa gttcaggcga ccacagttga
     7921 aggccgagtt gtccgggacc gcgtccttta acatcaacaa tgaagactgg gcgcgaccag
     7981 atgcaacgaa caaagcgaac agttcgatct caggctgcac ttcgccttat caacccttca
     8041 gtagcgagct aatggggctt tacaacgacc atccaaatgg tgcgctggaa actgatttgg
     8101 ggctgcctct taacgcaggt aattactggt gggcgtacga tcaaactgtt tcaagttcaa
     8161 catggtataa ccaggtgctt aatctgatgg acggtgctat ccgcaacgaa agcacaacat
     8221 cgacggcggg caatttaatg ctctgcctgg ttgacccgca cactgaggca acgaagattg
     8281 agttgacctc tacgttgctg aatgcggaca aaacaggcat caacgacaac cgtccgaccg
     8341 catgggcgaa gaagggcgaa tcaataccgt taaccatcaa ggtcacggat gatgcgggca
     8401 gacctgccgc ctatgaaagc gtcacgttgt cgcgtgcgac agcacaagtg cgcactacga
     8461 gtgatttcaa aagctcgaca gcagatgatc tgctgctaca gttcctgacg ccggttgcgc
     8521 aagccaatta caaatttgca gatgataacg ccagttggaa cggggttacg gacggtaatg
     8581 gcatcatcac gttaaccgct acgcaggata atacctgggg actggtaaca ccgtttacgg
     8641 cgacgcttgc gcgcaaaaca gcggtgaagt cagatctcga cctgatgtat accgtagtaa
     8701 caagcccgga tacgccaaaa gccaacttct ggggacatat gccagaaacg gtaagctcag
     8761 cagacggaac gcagtttgag cggatgaaac tgtgggccga aatgggggca accagcggtg
     8821 gcggttattc gctcactaaa gtgaatataa ataatgaaag ctggcctgcg ccatatatta
     8881 cccagcgata tttgccggag cttacccctt gtgaaaccgc acgatatcca tcgttggatg
     8941 agcttcaatc tttatataac cgctatccga atggctctat cgtcaatgag attggctggc
     9001 cggtgaaaca gaatagctat gtgtggtgga caaatgcaga ttgtcctgcg ggcgcgaatt
     9061 cttgtgaatc atttgacttc aatacaggtc aaattgttac tgtatctaaa agccgcgcct
     9121 tccaggcgtg cctggtgcac cctcactcaa cggtttctac cgtcaccctg acgtcaacgg
     9181 catttgactc caccgctcag gcggcgaaag tgaagaaggg cgaggcaatg ccagtcaccg
     9241 tgacggtgaa agacagcgcc gggaaacctg tgccaaatgt ggcgtttact ctgcgacgtg
     9301 gtgatgcctc tccgcgtaat agtggggcga cgctatatgg cgatgtggcg gctatggatg
     9361 atattaccat ccagccatca tcgggggcgg ctaccctatt ggcggttagt gggaacgaga
     9421 tcagtggcgt gacgggggca gacggcacgg caaactttac tgttcgtcag aacaatacgc
     9481 cgggttataa aacgccgctg acggtaacgc tggataaata ctcaacagtg actgcaatga
     9541 tggacactat tttcaccgta ttgaccagcc caaatgtatc ttctgctcac ttctgggggc
     9601 atatggcaga cacggcagtg gtgaatggaa aaacattaca tcgaccgata ctgacaactg
     9661 aattaccgtc cggggccaat cccgtatcct caccgattat taactacgaa aactgggctt
     9721 ctgcgcatat tactgatgcc agcaaatggg atatctcgaa gcagtgtgga aatatagaaa
     9781 atgcaccgac atacaatgag ttagagacat tgtatgctgt gtttaattcg ctgggatggc
     9841 cagcttcgcc aagtttccct tacttgtcct ctcaacggtg tgggatggac gagagcactg
     9901 gcacagagga ttgtagtatc acgttagcca ataaacctgg tctggttact tgtttccagt
     9961 gacgagggga gcgcatttgg cgtagcctgg gcgctattgc ctaatgcgat gctggcgcct
    10021 cttttccggc ctacgtgtta gcggcttttt tgcgcggttt gcggtttttt gttacggcgc
    10081 tgacttcact aatcaccacg ccatcaccta accgcgtggt cagcgaatcg cccgctttca
    10141 cctgcttaac ctgcttcaac actgtgccat cggttgtact ggtgacacta taaccacgcg
    10201 ccagcgtcga aagagggcta actgcttcga ggtgcgttac tgcattaccg aaacgttcac
    10261 gcgtggcgct aagctgtgcg cgcagggttt ctgctaaacg atattccagt tgctgaatgc
    10321 gcgtttgcgc gcgatgaatc ttcggctgtg gattttgctg attcagccgc tgtgttaatc
    10381 gctgctgctg ttgcccggca cgcttaagtt gattttccag cgcaaagctc atccgttttt
    10441 gcaggcgttc aagcatggtt tgctggcgtg ccagccggag ctgcggatgc tgttgctgta
    10501 atcggtgatg aatttgcgta aagcgacgtg tgcggttggc gagataataa tccatcgcca
    10561 tctccagccg ttgacgggtc gattgcacct ggcgcagtaa ctcttgctga ttacggctca
    10621 ccacttcggc ggcagccgac ggtgttggcg cacgcagatc ggcaacaaag tcggcaatcg
    10681 tcacatcggt ttcatgtccg acggcactca ctaccggaat gcggctggca aaaatcgccc
    10741 gcgctacgcg ttcatcgtta aaactccata aatcttccag cgaaccgccg ccgcgcccaa
    10801 cgatcaacac gtcgcactca ttgcgctgat tcgccagttc aatggcgcga acaatttgcc
    10861 ccggcgcgtc atcgccctga acggcggtgg ggtagatgat caccggcaga gaaggatcgc
    10921 gacgttttaa cacatgcaaa atatcatgta gcgcagcacc ggtttttgag gtgatcacac
    10981 caacgcaatg cgcaggggag ggaagtggtt ttttgtattg ctgatcgaac aaaccttcag
    11041 cctgcaactt cgctttgagc tgttcgtact tctgttgcag cagcccttca ccggccggct
    11101 gcatactctc aacgattatc tggtagtcgc cgcgcggctc gtagagcgta atattggcgc
    11161 gaactaaaac ttgttgccca tgctgtgggc ggaaggtcac ccggcggttg ctgttgcgga
    11221 acatcgcgca gcgtacctgg gcggtgtcgt ctttgagtgt aaagtaccag tgaccggaag
    11281 ccggttgtgt gaaattagag atttcgccgc tgatccaaac ctgtcccatc tcatgctcaa
    11341 gcagcagacg aaccgtttga ttcaggcgac taacggtaaa aattgcaggg gattgagaag
    11401 gtaacatgtg agcgagatca aattctaaat cagcaggtta ttcagtcgat agtaacccgc
    11461 ccttcgggga tagcaagcat tttttgcaaa aaggggtaga tgcaatcggt tacgctctgt
    11521 ataatgccgc ggcaatattt attaaccact ctggtcgaga tattgcccat gctacgtatc
    11581 gctaaagaag ctctgacgtt tgacgacgtt ctcctcgttc ctgctcattc taccgttctg
    11641 ccgaatactg ctgacctcag cacccagctg acgaaaacta ttcgtctgaa tatccctatg
    11701 ctttccgcag caatggatac cgtaacggaa gcgcgcctgg ctattgctct ggctcaggaa
    11761 ggcggtatcg gctttatcca caaaaacatg tccattgaac gccaggcaga agaagttcgc
    11821 cgtgtgaaaa aacacgaatc tggtgtggtg actgatccgc agactgttct gccaaccacg
    11881 acgctgcgcg aagtgaaaga actgaccgag cgtaacggtt ttgcgggcta tccggtcgtt
    11941 accgaagaaa acgaactggt gggtattatc accggtcgtg acgtgcgttt tgttaccgac
    12001 ctgaaccagc cggttagcgt ttacatgacg ccgaaagagc gtctggtcac cgtgcgtgaa
    12061 ggtgaagccc gtgaagtggt gctggcaaaa atgcacgaaa aacgcgttga aaaagcgctg
    12121 gtggttgatg acgaattcca cctgatcggc atgatcaccg tgaaagattt ccagaaagcg
    12181 gaacgtaaac cgaacgcctg taaagacgag caaggccgtc tgcgtgttgg tgctgcagtt
    12241 ggcgcaggtg cgggtaacga agagcgtgtt gacgcgctgg ttgccgctgg cgttgacgtt
    12301 ctgctgatcg actcctccca cggtcactca gaaggcgttt tgcagcgtat ccgtgaaacc
    12361 cgtgctaaat atccggatct gcaaatcatc ggcggcaacg tggcaacagc tgcaggtgca
    12421 cgcgctctgg cagaagctgg ttgcagcgcg gttaaagtcg gtatcggccc tggctctatc
    12481 tgtacgactc gtatcgtgac tggcgtaggt gttccgcaga tcactgccgt tgctgacgca
    12541 gttgaagcgc tggaaggcac tggcattccg gttatcgctg acggcggtat ccgtttctct
    12601 ggcgacatcg ccaaagctat cgccgctggc gcaagcgcgg taatggtggg ttccatgctg
    12661 gcaggtactg aagaatctcc gggtgaaatt gaactctacc agggccgttc ttacaaatct
    12721 taccgtggga tgggctctct gggcgcgatg tccaaaggtt cctctgaccg ttatttccag
    12781 agcgataacg ctgccgacaa actggtgccg gaaggtatcg aaggtcgcgt agcttataaa
    12841 ggtcgcctga aagagatcat tcaccagcag atgggcggcc tgcgctcctg tatgggtctg
    12901 accggctgtg gtactatcga cgaactgcgt actaaagcgg agtttgtacg tatcagcggt
    12961 gcgggcattc aggaaagcca cgttcacgac gtgaccatta ctaaagagtc cccgaactac
    13021 cgtctgggct cctgattctc ttcgcccgac ttcatgtcgg gcgatttata ttatctgttt
    13081 cacttgcctc ggaataagcg tcaatgacgg aaaacattca taagcatcgc atcctcattc
    13141 tggacttcgg ttctcagtac actcaactgg ttgcgcgccg cgtgcgtgag ctgggtgttt
    13201 actgcgaact gtgggcgtgg gatgtgacag aagcacaaat tcgtgacttc aatccaagcg
    13261 gcattattct ttccggcggc ccggaaagta ctactgaaga aaacagcccg cgtgcgccgc
    13321 agtatgtctt tgaagcaggc gtaccggtat tcggcgtttg ctatggcatg cagaccatgg
    13381 caatgcagtt gggcggtcac gttgaagcct ctaacgaacg tgaatttggc tacgcgcagg
    13441 ttgaagtcgt aaacgacagc gcactggttc gcggtatcga agatgcgctg accgcagacg
    13501 gtaaaccgct gctcgatgtc tggatgagcc acggcgataa agttaccgct atcccgtccg
    13561 acttcgtcac cgtagccagc accgaaagct gcccgtttgc cattatggct aacgaagaaa
    13621 aacgcttcta tggcgtacag ttccacccgg aagtgactca cacccgccag ggtatgcgca
    13681 tgctggagcg ttttgtgcgt gatatctgcc agtgtgaagc cctgtggacg ccagcgaaaa
    13741 ttatcgacga tgctgtagct cgcatccgcg agcaggtagg cgacgataaa gtcatcctcg
    13801 gcctctctgg tggtgtggat tcctccgtaa ccgcaatgct gctgcaccgc gctatcggta
    13861 aaaacctgac ttgcgtattc gtcgacaacg gcctgctgcg tctcaacgaa gcagagcagg
    13921 ttctggatat gtttggcgat cactttggtc ttaacattgt tcacgtaccg gcagaagatc
    13981 gcttcctgtc agcgctggct ggcgaaaacg atccggaagc aaaacgtaaa atcatcggtc
    14041 gcgttttcgt tgaagtattc gatgaagaag cgctgaaact ggaggacgtg aagtggctgg
    14101 cgcagggcac catctaccct gacgttatcg aatctgcggc gtctgcaacc ggtaaagcac
    14161 acgtcatcaa atctcaccac aacgtgggcg gcctgccgaa agagatgaag atgggcctgg
    14221 ttgaaccgct gaaagagctg ttcaaagacg aagtgcgtaa gattggtctg gagctgggtc
    14281 taccgtacga catgctgtac cgtcacccgt tcccgggacc aggccttggc gttcgtattc
    14341 tgggtgaagt gaagaaagag tattgtgacc tgctgcgccg tgctgacgcc atcttcattg
    14401 aagaactgcg taaagcggac ctgtacgaca aagtcagcca ggcgttcacc gtcttcctgc
    14461 cagtacgttc cgttggcgta atgggcgatg gtcgtaagta tgactgggtt gtctctctgc
    14521 gtgctgtcga aaccatcgac tttatgaccg cacactgggc gcatctgccg tacgatttcc
    14581 tcggtcgcgt ttccaaccgc attatcaatg aagtgaacgg tatttcccgc gtggtgtatg
    14641 acatcagcgg caagccgcca gctaccattg agtgggaatg atttgaccct gcactatgaa
    14701 tgaacaaaac cctctgttac tacagagggt tttttatctt caagaatcat aggcttgaag
    14761 ttagtaacat cgattaatta aaccagctgt ccgatttgtt ctcttctgct ttgcccacgc
    14821 ttttcatcag atcgcgaccg ccttcagtca tatttctgtt ggcgtcagct tcagattgca
    14881 ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc gataaattcg ttttcgcgct
    14941 taacgcgggc tgcttctttc gccagcgcca gtttttgttt ctgaatctct aagctgcgta
    15001 gctcatcttc ataactttga tcgcgttttt tgtccgcagt ggcttcggcg tccagtttat
    15061 cctgacgagc tttcttattt gccgctgccg ctcttttgtt agcggcggcc tgggcatttg
    15121 cgcgacgttg cttctcttgc tggatttccc tgttgcgctc cgcgacccat tcgtcatgct
    15181 gcctttgctc ttcattttta ccttgctgtt ccgcttctgc gacagcagag agtgcaatga
    15241 tgaggcgata gccggatagc ttaaggaggc taagatggcg caaagaaaaa ctttcttcat
    15301 gactcctcct gattattagc tcttttcagg acatttggta tttggctgaa tacgcgtttc
    15361 gttatacgtc gtggtaataa caacggctaa acctgtcgta aactggcact ctttacccac
    15421 ctgagtagag gtatacactt tggtgccttc cttatacgtt aaagaaacac cttccactaa
    15481 ggttttatca ttcaccatag aacccgctgc cgcgcctaca gctccgccgc caactgcacc
    15541 cgccgtcgtt ccggaattgc tgccagaccc gacgttgtgg ccgataacac cgccagcgac
    15601 tgcgccaata agcgcgccga aggcttgtgc gttccgttta ttttgggagt tgtctacggc
    15661 aacttttgcg ggaagaatgg aaataatatt aacggtttta gtttcttgtt tggtattcag
    15721 ttgatcggtt tgataaacat cggcggcatg atcgtcagca tttgactggc atcctgccag
    15781 agtgaatgac gctaacattg ccacaggcag aagacatttt ttaaatttca tcactattcc
    15841 ttgttataca cattacgaaa aataagacat ttaagatcat caaaagagga aagtaaatgt
    15901 attgcgtata aagggataga ttaaatttat taaaattcac ttg
//
LOCUS       DAZUGK010000102        15901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZUGK010000102 DAZUGK010000000
VERSION     DAZUGK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.102"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..171
                     /locus_tag="ACHY5W_002808"
     CDS             <1..171
                     /locus_tag="ACHY5W_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4447682.1"
                     /translation="PADILESDENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILR
                     ADGTWENIGGMK"
     gene            900..1556
                     /gene="pphA"
                     /locus_tag="ACHY5W_002809"
     CDS             900..1556
                     /gene="pphA"
                     /locus_tag="ACHY5W_002809"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS4447683.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPHSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPNRQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1557..1748)
                     /gene="yebW"
                     /locus_tag="ACHY5W_002810"
     CDS             complement(1557..1748)
                     /gene="yebW"
                     /locus_tag="ACHY5W_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFS4447684.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1853..2089)
                     /gene="yebV"
                     /locus_tag="ACHY5W_002811"
     CDS             complement(1853..2089)
                     /gene="yebV"
                     /locus_tag="ACHY5W_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFS4447685.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(2207..3646)
                     /gene="rsmF"
                     /locus_tag="ACHY5W_002812"
     CDS             complement(2207..3646)
                     /gene="rsmF"
                     /locus_tag="ACHY5W_002812"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFS4447686.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     HEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPSANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSRVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3726..6359)
                     /gene="yebT"
                     /locus_tag="ACHY5W_002813"
     CDS             complement(3726..6359)
                     /gene="yebT"
                     /locus_tag="ACHY5W_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFS4447687.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(6328..7611)
                     /gene="yebS"
                     /locus_tag="ACHY5W_002814"
     CDS             complement(6328..7611)
                     /gene="yebS"
                     /locus_tag="ACHY5W_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFS4447688.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGSRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7741..8238
                     /gene="msrC"
                     /locus_tag="ACHY5W_002815"
     CDS             7741..8238
                     /gene="msrC"
                     /locus_tag="ACHY5W_002815"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFS4447689.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8335..9033
                     /gene="proQ"
                     /locus_tag="ACHY5W_002816"
     CDS             8335..9033
                     /gene="proQ"
                     /locus_tag="ACHY5W_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFS4447690.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            9053..11101
                     /gene="prc"
                     /locus_tag="ACHY5W_002817"
     CDS             9053..11101
                     /gene="prc"
                     /locus_tag="ACHY5W_002817"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFS4447691.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEV
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            11293..12174
                     /gene="htpX"
                     /locus_tag="ACHY5W_002818"
     CDS             11293..12174
                     /gene="htpX"
                     /locus_tag="ACHY5W_002818"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFS4447692.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(12220..13593)
                     /gene="yebQ"
                     /locus_tag="ACHY5W_002819"
     CDS             complement(12220..13593)
                     /gene="yebQ"
                     /locus_tag="ACHY5W_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4447693.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINLPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            13770..14561
                     /gene="kdgR"
                     /locus_tag="ACHY5W_002820"
     CDS             13770..14561
                     /gene="kdgR"
                     /locus_tag="ACHY5W_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFS4447694.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14705..14944)
                     /gene="yobH"
                     /locus_tag="ACHY5W_002821"
     CDS             complement(14705..14944)
                     /gene="yobH"
                     /locus_tag="ACHY5W_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFS4447695.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            15103..15246
                     /gene="mgrB"
                     /locus_tag="ACHY5W_002822"
     CDS             15103..15246
                     /gene="mgrB"
                     /locus_tag="ACHY5W_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFS4447696.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            15321..15608
                     /gene="yebO"
                     /locus_tag="ACHY5W_002823"
     CDS             15321..15608
                     /gene="yebO"
                     /locus_tag="ACHY5W_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFS4447697.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         4201 a         3808 c         4062 g         3830 t
ORIGIN      
        1 ccggcagaca tcctggagtc agatgagaat ggcattatcc cggagcagga cagggtaatc
       61 acgcaggtgg tgatactgga tgcggataaa aagcagatac agtgcgtggt aagaccgctg
      121 caaatcctgc gtgctgacgg gacgtgggaa aatattggcg ggatgaagta acccgacagc
      181 ttcacaaaac cggagtccgg ctccggtttt gtgttgcaat gtccggggga tatttgttaa
      241 gcagattgta ttgtatgtaa aggcagagcc ccgaatttta ctgttgtaag tgggcctggc
      301 gggaatggta tcgccagcat cattggcttg ggtccagtaa aaagagacgc agaaagatgc
      361 acaataggca ctacgcgtca tgcatggatt aggtcgctca tcatttacac agtactgcta
      421 aaaaattatt aaggaaagcg cggcacgtcg tatgcaagaa cgtgccacgg ctggctgatg
      481 gacgttcgat agcgcgagtt tgaatgaaaa tcagccggag atgattttac ataattgcta
      541 cggaattatt caatacagga attgcttgtg tatgcatgga ttgacctgaa atattcccga
      601 aaatttctct aaaaaactcg aaaaaaatgg taactaattg aatgtattaa tatgtaatag
      661 tacgtgttag agattaaaag atgagcataa atttatttaa cacattaatt ctaaaagatt
      721 ttgttgtttg ttgacgaaaa caggaatcgt gttcggtctc tttttatctg ttaaaagcca
      781 gaagcatttc cttcgctgac tttatagtca accataacac acactctact gtctgagtcc
      841 agcgtttttt aacattcttg ttaagattat gtgatcttta gcgcgggagg aaaatattga
      901 tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga catatctggc
      961 tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg cattgtcgtt
     1021 ttgatccgtg gcgagattta cttatctcag tgggagacgt tatcgatcgt gggccgcata
     1081 gtttacgttg tctgcagtta ctggaacaac attgggttcg tgcggtaaga ggcaatcatg
     1141 aacagatggc gatggatgcg ctggcatccc agcagatgtc tttgtggttg atgaatggcg
     1201 gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg gcgctggaaa
     1261 aatgtcagca tttgcccttt attcttgaag tacacagccg caccggcaaa catgttattg
     1321 ctcatgccga ttatccagat gatgtttatg aatggcaaaa ggacgttgat ttgcatcagg
     1381 tcttgtggag ccgctcacga ttaggtgaac gcccaaatag gcagggaatt acaggtgctg
     1441 atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc aacctgcatt
     1501 atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg caataattaa
     1561 aaatcaccgt actcctgtgc aggtcgccag aaaccatcta taaaatcctc aatcggaaaa
     1621 caaccgccat ggcggatccg ttgatcgctc atagaatgaa gacactgctg ttccgtgttg
     1681 tagacatcca caacaatatc ttcacaaccg ccatccaggt agcaaacaaa aagtaccagc
     1741 gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa gtttaggaga gattttacaa
     1801 cgggggaata accaggacaa ataacccgcc atgccgacgg gttctttttg gatcaggcaa
     1861 gacgcataat ccaggcatca gattgttgat cgtaacgggt acggtaaagg acagagtgtt
     1921 cgccatttag cagggcaggg atactggttt cagcatccca ggcatccagt tgcatacata
     1981 aatcggggtc agatttacaa ggaatggtta acgttcgatc acccggcgat tcaccgtgta
     2041 attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc ttcgtgctct
     2101 ccattttgct gccgattgtg gtacgaccag tccgatcgca ccatttttca gcctagttga
     2161 ggccgccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcat gcgttactgg
     2221 taaaaagttt cccgtcccgc accagttcac gcgggtagct gtttttcagt cgcgaaccaa
     2281 tccgtttggc taaaccaatc ggctgatgct ggaaagtaac caacacatca tccgccactg
     2341 gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttct tgcggtgtca
     2401 gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca tgctgccagc
     2461 gataaccttt gttgtgcgtt tcggcaagtt taatccccaa ccgagaaaat cggactttac
     2521 cgatcagggc ttcaatgccc accgggaaca accacacttc tttgtcgcgc tgccagagtc
     2581 gcaggtttcc atcccagttt aagccaacac ttgcagccgc ctgacgaatt tgtgcggctt
     2641 cgcgatcttt cacccggctg aacgggaaat tgcccacttt gtattttgga gcgggtaagg
     2701 cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag tcgtaaattt
     2761 gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcactt gggaagagat
     2821 cgccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac aggcaaacgg
     2881 cttcgttttc ttcccggttt aaggtgcagg tcgagtaaac cagcgtaccg ccaggacgta
     2941 atgcatgaaa ggcgctgtca atcagttccc gttgggtagc tgcgatttca tgattgcttt
     3001 cgggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg ccggagcagg
     3061 gagcgtccag caaaatggca tcgaacattt ctggcactgc cgcaccaaac acgcggccat
     3121 caaaatgtgt gagcgcaaca ttactgatgc cacagcggct gatattggca tgtaacactt
     3181 ttacccgact ggcggaaaac tcattggcaa ggatcgctcc ttcgttattc atccgcgcgg
     3241 caatttgcgt cgtttttgag ccaggcgcag cagcgacatc catcacccgc tgtggtgcat
     3301 taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga atataaaaca
     3361 ggccgcttaa atgctcggcg gtactaccca atggcaatgc atcttcatcg tcgcgttcaa
     3421 tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa ggagcggtta
     3481 attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg cggcgcaacg
     3541 ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc gcttcgcgca
     3601 tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc atataccact
     3661 ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg cgcaggcacg ccggagcagt
     3721 gggcattatt tgggaagcgc agtaccccat tcacgccact ctttcggttc actttcctgc
     3781 aacaggaagt gcttgccttc ctgggctttc ggtgccagtg gcgtacccgg aggcgtggcg
     3841 aaggcgatgc cgccacggat aaactggtta aaggtgccgg ttttcactac gccgcccgtc
     3901 agaccaaagt ccagactgta acccgatgcc aaccagaaga cggaattgtt acgcaccagg
     3961 tgttgatagc gtttactgat gcgcatcgca atcatcacgc gatctgacaa tgtccccagc
     4021 gtcattccgg taaccgtacc gacttccaga ccacggaaca gcacaggcgt accgatgcct
     4081 aacgaaccgg cttccggcgc ttcaacaata atgcttaagc catccaggta acgcgaatca
     4141 gtaatggtgg cctcttgtaa ttcaaagtcg cggcgaggat tgccccggcc tggttcgacg
     4201 ttgatatacg gctggaggat agtatcaaga tgctcaacgc cagctgccga aatttgcggt
     4261 gtgaccactg agaagcgcgt accaccgcga gcaaaggtct ggacatattc cggatagagc
     4321 accgcctttg cctgtacttc attgcgcgcg gtaatcagat ccagcgtctg gatttgcccg
     4381 atatcaatac cgagatagcg aattggcatc ccgactgcca gttttccggc atcgaaagcg
     4441 tgaagcgtaa tctgcccgcc aaccgcacgg gccgctgttt cggaagcata cagaatacgc
     4501 ttatcgcctt tacgctgact ggcgctggca ccgctgaggt tatcgaagct aatggctccc
     4561 tttaatgctc tggagagcgg ggatgcctgt acggtcaggc cactaccatt cagctgaact
     4621 ttcgccccgc cttctgccca gaacacgcta ttgctggtca gaaggttgcg atactccggc
     4681 ttaatatgca gatcgatatc aaacgcgtta gctcgcggac gcacggtaat aacttcacca
     4741 acttcaaatt tacggtacag cactaccgat cctgcctgca catccggcag cgtctctgca
     4801 ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt tctccagcgc tttttccaga
     4861 ttggcataca gtggatagct ggctttcatc tcgcctttat cgcccggcag aatacgtatc
     4921 ccgccgttaa tccattctga ggcgctggca ccgagaaact caacgccatc cagccccacc
     4981 ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc cttttaccag ttctcgatgc
     5041 tgaggctcga tggcgacggt aaaggtgacg cctttgctgg tgagtttacg atcgataacc
     5101 tggcctacct gcacgccgtg aagaatgagc ggctgacccg catcaatacc gtaactttcc
     5161 ggtgcggtca gggtcagcgt cagaacatca ggttcatgca gcagtgcttt ttcgcctggc
     5221 acaacaacga actctttgcg tggctcgcca tcgcccggta ccaactcgaa ggttttgcca
     5281 gtcagcaggg cgctgagatt ggcatcgcta agggataatt tcggattacg taattcgatg
     5341 cgggtatttt cacgaagcag ggtaacgacg ctgggatcaa cggtcatttc tccggtgact
     5401 ttaccaccag gatttaaatc cagtttagtc agctgtccga cttccagccc ctgatacatt
     5461 aacggcgtcg agtcggcggt taatcctgcc ccactcggca gttccagttt tattattacg
     5521 ccacgctggc tgtgggccag atcttcatac agaccaaagg tatcttccgc ctcggcaggt
     5581 ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc caggctttcc
     5641 agtttcacct tcgcgccact gatactgacg ttggcatcaa cgccggaaac gttccagaaa
     5701 cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc aatcaccacg
     5761 ccttgtttat tgggattgat ggcatagtcg tagactttac ccaccgggat cttgcggaaa
     5821 tagaccaatg aaccgctgtt cagcgaaccg agatcggggg cttgcaggtg gatcatcaga
     5881 tcgccattgt ccagccgata tttcggttgg gtatcgagcg cgacaaagtg atcctgctct
     5941 ttacctttac ccggcatcat gccgatatag ttcccaccga cgagggcgtc cagcccggag
     6001 acacctgcca acgatgcttt tggcgtcacc agccagaact gagtctcttc gcgcagcgca
     6061 tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc gtcgctgagg
     6121 ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt acggcctgga
     6181 acaataccat ccgccgacat aaagtcgatg gtgacggtat taccccggtc ctgataactg
     6241 tcccaaatca gccaaccggc aatcattagc gcgatgaacg gcagcagcca gaaaggtgag
     6301 atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg
     6361 tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca aaattaccgc
     6421 tgcgccgaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat cgcgattaat
     6481 cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgac caatccaggt
     6541 caccattcgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct
     6601 caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac tggcgataaa
     6661 cacgattccc gcaacggcaa tgttgctact tgccagcgac ataatcccgg aaagaatcgt
     6721 atcttcctgc cggctaccat tcagataaat gatagaaata ggcaacaggt tggcaggtaa
     6781 caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat gacggcgacg
     6841 caggcgtagc ggaatatggc aacgcgggca gcgaccacgc tgatcaggat agccggtaaa
     6901 atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc gctgcggata
     6961 aaatcgctcc cacagctcct cgacattaag atgtgacaac gtcaccgtcg tcaaaatcac
     7021 caacgccaca aaagagaaca agccgacacc cgcctggata tgggcataat cctgtacctt
     7081 tatagaagca acgccaatgc cgaccaggta gatatcgagc attacccact ctttaagtcg
     7141 ctcaagcatc agcagtaccg ggcgtagatt cattcccagt cggttaccaa accataaata
     7201 agctatggag gtcaccagaa tgaggggggc accaataacg cagaaaaaga ccatcgaccc
     7261 cgttatcgca tcgccctgtt tggtcatttg ccagatgcct tgcatgacat tggcgtcgat
     7321 acgaatacct aacagccaga tatgcaacag cggttcgccc caggcaaaag gcatcagcaa
     7381 cagcatggtg aaagccattg ccgccaggcg cgttagcgac cagtcgcgcc cgtcacgaat
     7441 ttttgcctga cagcgcggac aataggcact ttgatgagaa tttatctcgg gcaggctaaa
     7501 cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct cgccgattgc
     7561 cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca
     7621 aatgtgataa gaattaacat taatcttaac ccatgatggg aatgatcttg agcattaacg
     7681 catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag tgattatata
     7741 atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct gatggcggga
     7801 gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatatga gcgtctcact
     7861 gacgtaaact gggcaggttt ttatttgctt gaggacgata cactggtact cggaccattt
     7921 cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gcgtgtgcgg cactgcggtt
     7981 gcccgcaatc aagtgcagcg tatcgaggat gttcatgtgt ttgacgggca tattgcttgt
     8041 gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca gattattggt
     8101 gttctcgaca tcgatagtac cgtcttcggt cgctttacag acgaggacga gcaaggctta
     8161 cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa aaaattcttt
     8221 gcgagcgtcg caggataatc aacggataac gtagcattta ctgatagcgt cattataatg
     8281 acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa
     8341 aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga acgttttccc
     8401 cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa tcggtatttt tcaggatttg
     8461 gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc cgctttacgt
     8521 ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac acgtgtcgat
     8581 cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc tcgcaagcag
     8641 cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc
     8701 gaagctgcag cagctgctgg tgagaaagaa gacgcaccgc gtcgcgaacg caagccacgt
     8761 ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta
     8821 gagaaagcgc caaaaacagc aaaagcacct cgcgaagaac agcacacccc agtttctgac
     8881 atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat
     8941 gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc gggtatgtct
     9001 ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag gcatgaacat
     9061 gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga ccttcgctgt
     9121 agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc agcatgcgac
     9181 ggtgagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt tcgacctcga
     9241 tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt acagccacaa
     9301 cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga
     9361 actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc aaaagcgtcg
     9421 ttttgaacgt taccagtacg ctttgtcggt actggaaaag ccgatggatt tcaccggcaa
     9481 cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg ttgagttgaa
     9541 cgcgctgtgg gacagtaaag tcaaattcga cgagttaagt ctgaagctga caggaaaaac
     9601 ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc gtcgtctggc
     9661 gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc gtgaaatcga
     9721 cccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg aaatgagttt
     9781 gtcgctggaa ggtattggcg cagtgctgca aatggatgat gactacaccg ttatcaattc
     9841 gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg acaaaattgt
     9901 cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt
     9961 ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt tacctgctgg
    10021 taaagggacc aagacccgca ctgtaacatt gacccgtgaa cgtattcgtc tcgaagaccg
    10081 cgcggttaag atgtcggtga agaccgtcgg taaagagaaa gtcggcgtac tggatattcc
    10141 gggcttctat gtgggtttga cagacgatgt caaagtacaa ctgcagaaac tggaaaaaca
    10201 gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc
    10261 ggtatcgctc tcaggtctgt ttattcctgc gggtcctatt gttcaggtcc gtgataacaa
    10321 cggtaaagtt cgtgaagaca gcgataccga cggacaggtt ttctataaag gcccgctggt
    10381 ggtactggtt gaccgcttca gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga
    10441 ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata
    10501 ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag cgctgggttc
    10561 tgtgcagtac acgatccaga aattctatcg cgttaacggt ggcagtacgc aacgtaaagg
    10621 cgtaacgcca gacatcatca tgccgacggg taatgaagaa acggaaacgg gtgagaaatt
    10681 cgaagataac gcgctgccgt gggatagcat tgatgccgcg acttatgtga aatcaggaga
    10741 tttaacggcc tttgaaccgg agctactgaa ggaacataat gcgcgtatcg cgaaagatcc
    10801 tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa
    10861 tatcgtttct ctgaattacg ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg
    10921 tctggcgcgt ttgaacgaac gctttaaacg cgaaggtaaa ccggagttga agaaactgga
    10981 tgatctaccg aaagattacc aggagccgga tccttatctg gatgagacgg tgaatatcgc
    11041 actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgcac ccgtcaagta
    11101 atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga tgccgtaaat
    11161 cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat ccagacttga
    11221 aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt
    11281 aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc
    11341 gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca ggggctgatg
    11401 atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat gtccaaatgg
    11461 atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga aagggaacgt
    11521 tggctggtca atactgtagc aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg
    11581 gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct
    11641 ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc cgaggcggta
    11701 attgctcacg aaatcagcca catcgccaat ggcgatatgg tcaccatgac gctgattcag
    11761 ggcgtggtga ataccttcgt tatctttatt tcccgtattc tggcgcagct tgccgcgggt
    11821 tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct gatctacttt
    11881 gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat caccatgtgg
    11941 ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt tggtcgcgag
    12001 aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga agcaaccagc
    12061 atgatggctt tctgcattaa cggtaagtcg aaatcgctca gtgagttgtt tatgactcac
    12121 ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa
    12181 gtaaatacta aaaagcgcgt cctgctcgac gcgctttttt tatgccctgg atcttggctg
    12241 agtgatacgt aacccactga tacaggcagc aatcactgcc agaatagccg cagccatcag
    12301 cgagacgtgt gtaccattat ctccaaactg atttagcatc agcgccacca gcgccgcgcc
    12361 gctactctga cccagtagac gagccgttcc taacatgcca ctggctccac cgctacgttc
    12421 gcgaggagcg gaggtaataa tggtgtggtt attgggtgac tggaacaagc caaatccagc
    12481 gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg gcagcagaac
    12541 cagggaaaaa agccccgcag ccatgatgaa taatcctaaa gcacccagca atcctgcatg
    12601 tacgcgttca atcaaatagc ctgccaacgg agccatcacc atcgttgcta acggccacgg
    12661 tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca ggtaaaaggg
    12721 cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag
    12781 tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa gagaaagctg
    12841 gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag caattaacgt
    12901 cagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga aggttaacgc
    12961 gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg
    13021 cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtaggttga ttaaaaataa
    13081 ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg cagcagaaga
    13141 gacggcaaca ataaacgagt ttatgcccat ccctctaccc agaaaacgtt gtggatagat
    13201 caggcggata agtgcggtat ttacgctcat caacgctgca ccgccgaaac cttgtatgac
    13261 acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg aagacaacag
    13321 aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc ccagaaacga
    13381 aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc agatggaact
    13441 ggccggcgtg gcatgaaggt ccgtggcgat tgttggcaag gcgacgttgg cgattgcgcc
    13501 gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt atcgctgggg
    13561 caatggcagg ccgtcggcct gaacttttgg cataggaatt ttaaatcttt ggtgaataat
    13621 caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat tgtaactaac
    13681 cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac agttctgatt
    13741 tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat aaacagcctg
    13801 attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt gaagagcgcg
    13861 aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct
    13921 ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg gagaaatatt
    13981 ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc
    14041 gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact atccacctcg
    14101 gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg tacaatttgc
    14161 gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac
    14221 tgctagcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg gagtataaac
    14281 gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg gaccaggtgc
    14341 gcgagcaggg gtatggcgaa gataacgaag agcaggaaga agggctgcga tgcattgcgg
    14401 taccggtatt tgatcgcttt ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt
    14461 tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca
    14521 aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa gaacctgtcg
    14581 gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga aggtggcgtt
    14641 atattaaacg cgcttgctat aagagtattt tactcaggag tgagaatctg gttatttatt
    14701 gcccttaacc attatcgacc acgatattgc ttttgcgtaa cagcgggcaa tctgttatcc
    14761 ccaaaaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    14821 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    14881 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    14941 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    15001 aagcgactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    15061 atgctagttt aatgacataa ggtaggtgaa acggagattg gagtgaaaaa gtttcgatgg
    15121 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    15181 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    15241 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    15301 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    15361 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttattaatc
    15421 gtgccagttc tcggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    15481 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    15541 aaaagaccat tgagagtcaa aaatcggttg aagacgaaga tattattcgc ctggtcgccg
    15601 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    15661 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    15721 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    15781 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    15841 tttctctctt gatttttttg cttattcatc attttttctt attttaaatt tccaatacct
    15901 t
//
LOCUS       DAZUGK010000103        15813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZUGK010000103 DAZUGK010000000
VERSION     DAZUGK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.103"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..317
                     /gene="bhsA"
                     /locus_tag="ACHY5W_002824"
                     /gene_synonym="ycfR"
     CDS             60..317
                     /gene="bhsA"
                     /locus_tag="ACHY5W_002824"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFS4447698.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHY5W_002825"
     CDS             complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHY5W_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFS4447699.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKNAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1505..4951)
                     /gene="mfd"
                     /locus_tag="ACHY5W_002826"
     CDS             complement(1505..4951)
                     /gene="mfd"
                     /locus_tag="ACHY5W_002826"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4447700.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5079..6152)
                     /gene="ycfT"
                     /locus_tag="ACHY5W_002827"
     CDS             complement(5079..6152)
                     /gene="ycfT"
                     /locus_tag="ACHY5W_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4447701.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMLTASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLQVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            6414..7613
                     /gene="lolC"
                     /locus_tag="ACHY5W_002828"
     CDS             6414..7613
                     /gene="lolC"
                     /locus_tag="ACHY5W_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFS4447702.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7606..8307
                     /gene="lolD"
                     /locus_tag="ACHY5W_002829"
     CDS             7606..8307
                     /gene="lolD"
                     /locus_tag="ACHY5W_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFS4447703.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIAKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8307..9551
                     /gene="lolE"
                     /locus_tag="ACHY5W_002830"
     CDS             8307..9551
                     /gene="lolE"
                     /locus_tag="ACHY5W_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFS4447704.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9580..10491
                     /gene="nagK"
                     /locus_tag="ACHY5W_002831"
     CDS             9580..10491
                     /gene="nagK"
                     /locus_tag="ACHY5W_002831"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFS4447705.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10507..11328
                     /gene="cobB"
                     /locus_tag="ACHY5W_002832"
     CDS             10507..11328
                     /gene="cobB"
                     /locus_tag="ACHY5W_002832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFS4447706.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11484..12530)
                     /gene="potD"
                     /locus_tag="ACHY5W_002833"
     CDS             complement(11484..12530)
                     /gene="potD"
                     /locus_tag="ACHY5W_002833"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFS4447707.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12527..13321)
                     /gene="potC"
                     /locus_tag="ACHY5W_002834"
     CDS             complement(12527..13321)
                     /gene="potC"
                     /locus_tag="ACHY5W_002834"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFS4447708.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13630..13833
                     /locus_tag="ACHY5W_002835"
     CDS             13630..13833
                     /locus_tag="ACHY5W_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447709.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            13811..14278
                     /locus_tag="ACHY5W_002836"
     CDS             13811..14278
                     /locus_tag="ACHY5W_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFS4447710.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(14356..15474)
                     /locus_tag="ACHY5W_002837"
     CDS             complement(14356..15474)
                     /locus_tag="ACHY5W_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4447711.1"
                     /translation="MSPRPRKNSTDVAALYEKFDRRTGKVYYQYKNPVTGKFHGLGTD
                     KVKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRTQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(15443..15712)
                     /locus_tag="ACHY5W_002838"
     CDS             complement(15443..15712)
                     /locus_tag="ACHY5W_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4447712.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGEAGIAPEIHTGDDDALKEILTHVTEATKKQY"
BASE COUNT         3753 a         3978 c         4098 g         3984 t
ORIGIN      
        1 tagttatcga tcgttaagta attgcttgcg acgtcattca tctgcataag gccactatta
       61 tgaaaaacgt aaaaaccctc atcgctgcgg cgattttaag ctccatgtca tttgccagct
      121 ttgcggctgt cgaagttcag tcaacgccag aaggccaaca aaaagtcggt acaatcagtg
      181 ctaacgcggg gacaaatctg ggatcgctgg aagagcagct ggcgcaaaaa gcggatgaga
      241 tgggcgcaaa atctttccgt attacttctg taaccggtcc gaataccctc catggaacag
      301 cagtaattta taaataagca ttaaccctca ttaatgcctg ctactgctga ttttttcccc
      361 gcgacatgcc gtgtcgcggg gattttttta tccaggattt acagagtttg tgggctcact
      421 tgatgacggc ggacatccac cggcatcccg gaacggacat ccatcacatg ttgcatcact
      481 tcagcgtcag tttgtgctgc atttttaaat gattgcattg cgctattcag cgtaattggc
      541 agcagctgcg gatcgtcatc aatcttctct gacagcggct gatgtacttc aaccagacgc
      601 gcaccgtttg gttcggcaga gacttttatc ggagtgttga tgatattcac tttggtgcct
      661 ggggtgacct ggctaaagag tgttttgata tcgtcatccc gcagacgaat acagccagaa
      721 cttacccgca tgccaatgcc gaaatcggcg ttcgtaccat gaagcaaata aacgccgcca
      781 taggccgcca gacgaatcgc atgatggccc attgggttat ccggtccagc cggcactacc
      841 gcaggcaact caattccctg tgctttataa cgggcgcgga tgtttgccgt tggcgtccag
      901 gttgggtttg cacgtttgtc tgaaacggtg gtcaccattg tcggtgtcag cgtgtcacca
      961 cctaactgac caatacctat tggatacacg gttaccgaat ttttacccgg cgggtagtaa
     1021 tagagacgca gctcagcaat gttgatcaca atgccttcac gcggcgcgtc tggaagtagg
     1081 gtttgcaacg ggatcgttaa cacgctgccc gcgcgcggta cgtaaggatc aacgccgggg
     1141 ttagcctgta acagagcgag aaagccgacg ttatattttt tggcgatggc ttccagagaa
     1201 ccaccgtcat tttccaccac atgaaatttg ttttcgccaa ccagacgact gcccgctggc
     1261 ggcagcggcc aggtgttggc ttttgccggt agcgccagcg ccacggcagc ggcgaacgta
     1321 aaaaacgtta gccagcgaga aaaacgcgtt ttgatcatca ccaaaaatcc ataataaata
     1381 taaggttatt gtaataaaca gataagcctg aattatggat ggtgacagtg tcggatagtg
     1441 cagggaagtg caaagaattt gtaaatgttg cagatggggg cgcagaaacg cccccgattt
     1501 accattaagc gatcgcgttc tcttccagtt cacgcataaa ctggcgtacc cattcgatac
     1561 gcgttttccg ctcactcaaa tcctgaataa atttcaggcg cgtcggacca tcaaggcggt
     1621 aatgctgcgg ctgtttttgc agcaaaccaa tcaaccaggc cggattaacg tgattcttct
     1681 cggcaaattc gatcaccccg cctttctcat taccttccag cttcctgatc cccagtttct
     1741 gcgcttgctg gcgcagacgg gcaatatcca gcagggtacg cgccggatcc ggcagcaggc
     1801 cgaagcgatc gataagctcg actttgatct cttccagttc gttttccgtt ttggcgctgg
     1861 caatacgttt gtagaacgac agacgcgtgt tcacgtcagg gatgaaatca tctggcaata
     1921 gcgacggcat ccgcagctcg acttctgttt gctggctggt gagatcttcc agcgacggct
     1981 cgcgtccggc tttcagtgca tcgacggcgt tttccagcaa ctccatatac agcgagaaac
     2041 cgatggtttc cattgagccg ctttgttctt cgccaagcag ttcacccgcg ccgcgaatct
     2101 ccagatcgtg cgttgccagc gcaaaacctg ccccgagatc ttccagcgag gcaatcgctt
     2161 caagacgttt ttgtgcatcg gtagtcatcg cttttggatg cggtgtcagc aaccatgcat
     2221 atgcctgatg atgcgaacgt ccgacgcgac cgcgtaactg gtgcagctgc gccagaccga
     2281 agtgatccgc gcgttcaatg ataatggtgt tggctgtcgg gatgtcgatc ccggtttcga
     2341 taatggttgt acaaaccagc acgttgaaac gttgatgatg gaaatcattc atcacccgtt
     2401 ccagttcgcg ctcgcgcatc tgcccgtgac cgatggcgat ccgcgcttct ggcaccagtt
     2461 ctgccagccg ttcggcggct ttctgaatgt tttccacatc attgtagaga taataaacct
     2521 gccccccgcg caaaatttca cgcaggatcg cctcccggac caccaggctg tcatactcac
     2581 ggacaaaggt tttaactgcc agacgacggg cgggcggcgt ggctataatc gacaggtcac
     2641 gcattccgct cattgccata ttcagcgtac gtgggatcgg cgttgcagta agcgtcagaa
     2701 tatccacgtt cgcgcgcatc gctttaatgc gctctttatg acgcaccccg aagcggtgtt
     2761 cttcatcgac aatcagcagg cctaaatctt taaacttgac gtcactttgc agcagtttgt
     2821 gcgtaccgat cagaatatcg attttccctt ccgccacttc cgcaaggatt tgcgtctgct
     2881 ctttggcgct gcggaaacgg gagatcattt cgatacggac cggccagttg gcgaaacggt
     2941 cgcggaagtt gtcgtaatgc tgctgcgcga gaagggtggt aggcaccagc accgccacct
     3001 gcttgtggtt atctactgcc aggaaagcgg cgcgcattgc cacttctgtt ttaccaaagc
     3061 caacatcgcc gcacaccaga cgatccattg ccagcggctg acacatgtcg ctaagtaccg
     3121 cattaatggc ctgcgcctga tccggcgtgg tttcaaacgg gaagctgtcg cagaacaact
     3181 gatactgctc acgatcgtgt ttaaacgcga agccctcttt ggcggcgcgt tgcgcgtaga
     3241 tatccagcaa ttccgccgcc acatcacgca ctttttccgc cgctttctgc cgcgcgcgtg
     3301 accacgcatc gccgccaagt ttatgcagcg gggcgttttc ttccgcgcca cctgcgtaac
     3361 ggctaatcag atgcagtgac gacaccggaa catacagttt ggcgtcgttg gcataggtga
     3421 gcatcaggta ctcgccagta atgccgccag cttccagcgt ggtcattccg gcatagcgac
     3481 cgacgccgtg ctccagatgg accaccggct ggccaatatg cagctccgcg aggttgcgga
     3541 tcagtgtatc ggggttgatg gtgcggcgag aatcctgacg acggcgggca acgcgttcac
     3601 cgagcagatc gctttcgcaa atcagcgcca ggttacgcac cgtatcgaca aaaccatgct
     3661 ctgcagcgcc aatcatcaaa taacgcccac ggtcgctggc ttcatcaaga cgcataatgc
     3721 gttgcggagc aattttaatt cgtgcgagca gttcacccag cgcttcacgg cgaccttcac
     3781 tttctaccga gaacaccacc ggaccgtcga aagtctcgag gaacttacgc agcgcatcca
     3841 gcggcgcttt ttgttgtgcc tgaacggcca ggtctggcag tttctggaaa cctaaattgg
     3901 cattcgcggc ttttgtcggt aaatgttcag tttttagctg cactcggggc cagtttttca
     3961 gctctgagaa gagctcgtcc acccgcagcc agagcgattg tggtggcaac agtgggcgca
     4021 tcggatcgac gccgcgattc tcaaaacgcg ccagcgtgtc agcctggaaa cgttcggcac
     4081 tgttttccag atcgccagta ttcaccagca aggtattggc agggaaataa ctgaacagcg
     4141 gcggcagtgg ttcgctgaag aacaatggct gccagtactc gatcccggct ggtaatgtgc
     4201 ctttactcac ttgctggtaa atatgttccg gatcgcgctt cacttcgaag gtatcgcgcc
     4261 actggctgcg gaacagttca attgccgctt tatcggtcgg aaattcgtgc gcgggcaaca
     4321 gattaatcgc ttctacttcc tccagcgtgc gctggctgtc gacgtcaaac acccgcaggc
     4381 tgtcgatttc atcatcaaag aaatcaagac gataaggcag ctcactcccc atcgggaaga
     4441 gatccagcaa cgcgccgcgc gtggcgtatt cgccgtgctc catcacctgg tcaacatggc
     4501 gataaccggc gctgtccagt tgggttcgta atgcatctcg tgacaggcgc tgaccttttt
     4561 tcatcaccag cgcatgaccg tggagaaaac tgtgtgggca aacgcgctgc ataagcgtat
     4621 tcaccggaac aatcagtacg ccacgctgca tcgtcggtag ctggtaaagg gtggaaaggc
     4681 gcgaggagat aatgtcctga tgaggcgaaa aactgtcgta agggagggtt tcccagtccg
     4741 caagattcat caccatctga tcggtgaact ggctgatttc atcatgcaaa cgcagagcat
     4801 tttgcatatc tggtgcaatg agtaccaccg gaccggcgtg acgttcggca atttccgcca
     4861 ccagcgtcgc gcaggcagcg ccggttaact cgcccagcag acgctgctca cccgctttga
     4921 cgggcagcgt ataacgatat tgttcaggca taacggttgt cagattctcg ttaggatatg
     4981 cctcaacata tgggggcata tctctgataa gcaatgctgt tcattatccg taatcgacat
     5041 ggtttagcaa attgaatcgc cccggctggg gcgatggctt agcgggagta actgatggcc
     5101 gcgggcagag aaggcgggga gaacaacaga tcgctgaatg cacgctgtga aagttttctc
     5161 acctgcaagc ccgcaacagt acagatgaac aaactgacaa agggataaac cagcaggagt
     5221 gtcagttcga cttgcggcga ccagcgtgct gcgttcattt gcgcaagcag agttaagctg
     5281 aatatttcaa ccagaatgcg atgggtggtg tagatagcaa tggtgttgga gccaatcaca
     5341 ttcagcaggc tggtggagcg catgccgaat cgttgctcgt attgataaaa cagcttcatg
     5401 atcaccacaa tcgataccag cgacaacagc agcgagatag taaacaacca ggcaccgacc
     5461 gccagaacgg taagcaaaga agccatcagc agatggcggc gcaacggcac ctctttaaca
     5521 caggtcatta ttgttgcgcc aaaccatgcg ccaaggctgt aataaggcaa attgcggatc
     5581 acactgttca ttccccacca cggcgtggga acgaaattaa ccgccacact cagcagtaca
     5641 aacaaggcga atagtggcag cgccagacgg ctaaaaattt tacataccac gaaatagaca
     5701 attaacgcat acagatacca caagctggtg ctggcggtga gcatcccgtg caggaactca
     5761 ccggtggaat cggcatacgc ggcattggaa gcgttgctta aatcgcgctc aggtgccagc
     5821 cactggttca gcgcacttaa cgccagccac tgcaccacgc cccaaagcgc cagcacccaa
     5881 aagatgctcc agatgcgttt atcgagacaa tttccccacg gtacgctgtc gatatagcgg
     5941 cgaattaaat agccggaaat aaagaaaaaa accggcatac gaaagggagc aaggtaaaga
     6001 ttgaaataga tccagcattt gctcaggact tccgataacg gatgctgaaa agtggtcaga
     6061 tgcggataaa aggtaatgac cgagtgataa attactacca gacaaataca taaccctttg
     6121 atctggttaa tccatagctc tttttgtttc atcaggacaa catgctcttt tttaatttac
     6181 atacggttga tcctgcgggt tatggcccta tgtgtaatgg gtatctgtct gtattggatg
     6241 atttttcaga ttaagatcag gcggcaagat tgatgataaa acatggcaat ttagccgatt
     6301 gatttacggg ggcttttcag attagccctg acgatcactt acagttcaga cgtttaccca
     6361 tcttgctttc gcttatatac tcgtgtcttt gctacagcaa ccagacggat ttcatgtacc
     6421 aacctgtcgc tctatttatt ggcctgcgtt acatgcgtgg gcgtgcagcg gatcgcttcg
     6481 gtcgtttcgt ctcctggctt tctaccatcg gcattaccct cggggtgatg gcgctggtca
     6541 cagtattgtc agtgatgaac ggctttgaac gcgagctgca aaacaacatc cttggcctga
     6601 tgccacaggc aattctctct tctgagcatg gctctcttaa cccgcagcaa ctcccggaaa
     6661 cggcagtcaa actggacggc gttaatcgcg tcgcacctat tactaccggt gatgtggtac
     6721 tgcaaagcgc gcgcagcgtg gcggtcgggg tgatgctcgg tatcgacccg gcgcaaaaag
     6781 atccactaac gccgtatctg gtcaatgtga aacaaactga cctcgagccg gggaaatata
     6841 atgtcatcct cggcgaacaa cttgcctcac agctaggcgt taatcgcggt gatcaaatcc
     6901 gcatgatggt accatctgcc agccagttca cgccgatggg gcgtattcca agtcagcgcc
     6961 tgttcaatgt gattggcact ttcgccgcca acagtgaagt cgatggcaat gaaatgctgg
     7021 tgaatattga ggatgcctcg cgtctgatgc gttatccggc aggcaatatt accggctggc
     7081 gtttgtggct ggatgagccg ctgaaagtcg actcattaag tcagcaaaaa ctgcctgaag
     7141 gcagcaaatg gcaggactgg cgtgatcgta aaggcgagtt gttccaggcc gtacgcatgg
     7201 aaaaaaatat gatgggttta ctgctgagcc tgattgtcgc cgttgcggcg tttaacatta
     7261 ttacctcact agggctgatg gtaatggaga agcagggcga agtagcgatc ctgcaaacgc
     7321 aaggcttaac tccgcgacaa atcatgatgg tctttatggt gcaaggggcc agcgccggga
     7381 ttatcggtgc gatcctcgga gcggcgcttg gcgccctgct tgccagccag ttaaataatc
     7441 tgatgccgat aatcggcgtc ctgcttgatg gcgcggcgct gccggtcgct atcgaacctt
     7501 tacaggtcat tgttattgcg ctggtggcga tggctatcgc gctgctgtct acgctttacc
     7561 cttcatggcg cgctgccgcc actcaacccg ctgaggcttt acgttatgaa taagatcctg
     7621 ttgcaatgcg acaacctgtg caaacgctat caggaaggca gtgtgcaaac cgatgtgctg
     7681 cacaatgtca gtttcagcgt cggcgaaggt gaaatgatgg cgatcgtcgg tagctctggt
     7741 tccggtaaaa gtaccttgct gcacctgctg ggcgggctgg atacgccaac ctccggcgat
     7801 gtgattttta atggtcagcc aatgagcaaa ctgtcgtcgg cagcgaaagc agaactgcgc
     7861 aaccagaagc tgggctttat ttatcagttt caccacctgc tgccggattt taccgccctg
     7921 gaaaacgtgg ctatgccgct gctgattgcc aagaaaaagc ccgctgaaat caacagccgt
     7981 gcacttgaga tgttaaaagc ggtggggctg gatcatcgtg cgaatcaccg cccgtcagag
     8041 ctttctggcg gcgaacgcca gcgtgtggcg attgcccgtg cgctggtcaa taacccgcgt
     8101 ctggtactgg cggatgaacc aaccggtaac cttgacgcac gtaacgccga cagcatcttc
     8161 cagttgcttg gagaattgaa tcgcttgcag ggcaccgcct tcctggtggt tactcacgac
     8221 ctgcaactgg cgaaacgtat gagccgccaa ctggagatgc gtgatggtcg tctgacggcg
     8281 gaactgagcc tgatgggggc ggagtaatgg cgatgccttt atcgttattg attggcctgc
     8341 gttttagccg cggacggcga cgcggcggca tggtgtcgct gatctccgtc atttctacca
     8401 ttggcattgc ccttggcgtg gcggtattga tcgtcggctt aagcgcgatg aacggctttg
     8461 aacgcgaact gaataaccgc attctggcgg tggtgccgca tggcgaaata gaggcggtgg
     8521 atcaaccgtg gactaactgg caggaagcac tggataacgt gcaaaaagtg ccaggtattg
     8581 ccgccgctgc gccgtatatc aatttcaccg ggctggtaga aagtggtgcc aatctgcgcg
     8641 caatccaggt gaagggcgtt aacccgcaac aggaacagcg tctgagcgca ttaccctcgt
     8701 ttgttcaggg ggatgcctgg cgcaatttta aagcgggcga acagcaaatt atcatcggca
     8761 aaggcgtggc ggatgcgctg aaagtgaagc agggcgattg ggtgtcgatc atgatcccca
     8821 actcgaatcc tgagcataaa ctgatgcagc caaaacgtgt gcgtttgcac gttgccggta
     8881 ttttgcagtt gagtggtcaa ctcgatcaca gttttgccat gatcccgctg gcggatgccc
     8941 aacaatatct tgatatgggt tccagcgtgt caggtattgc ccttaaaatg acggatgttt
     9001 tcaacgccaa taagctggta cgcgatgcgg gtgaagtgac caacagctat gtttatatta
     9061 aaagctggat tggtacttac ggctatatgt atcgcgatat ccagatgatc cgcgccatta
     9121 tgtatctggc gatggtactg gtgattggcg tggcctgttt caacatcgtc tccaccttag
     9181 tgatggcggt gaaagacaag agtggcgata tcgcagtatt aagaacgctg ggggcgaaag
     9241 atggtttaat tcgcgccatc tttgtctggt atggattgct ggcagggcta ttcggtagcc
     9301 tgtgtggtgt gattatcggc gtagttgttt cactgcaact taccccgatt attgagcgga
     9361 ttgaaaagct gatcggtcat cagttcctct ccagcgatat ctattttatt gatttcctgc
     9421 catcggaatt gcactggctg gacgtcttct acgtactggt cacagcattg ttgctgagtc
     9481 ttttggcaag ttggtatccg gcgcggcgcg ccagtaatat tgaccctgcg cgagtcctta
     9541 gcggccagta aaggcagtac attaaaacaa ggagcggcaa tgtattacgg gtttgatatt
     9601 ggtggaacaa aaattgcgct tggcgtgttt gatagcggtc ggcagttgca gtgggaaaag
     9661 cgggtgccga caccgcgtga tagctatgac gcatttttag atgcagtgtg cgagctggta
     9721 gccgaagctg atcaacgttt tggctgtaga ggttctgtcg gcatcggtat tccgggaatg
     9781 ccggaaacag aagatggtac gctgtatgcc gccaatgtcc ccgctgccag cggtaaaccg
     9841 ctgcgtgccg acctgagcgc acgtcttgat cgcgatgtac gccttgataa cgatgccaac
     9901 tgttttgccc tttcagaagc ctgggatgat gaatttacgc aatatccact ggtgatgggg
     9961 ttgattctcg gcaccggcgt tggcggcggg ctgattttca acggcaaacc gattaccggt
    10021 aaaagctaca ttaccggcga gtttggccat atgcgtctgc cggttgatgc gttaaccatg
    10081 atggggttgg atttcccgtt acgccgctgc ggctgtggtc agcatggctg cattgaaaat
    10141 tatctgtctg gtcgcggttt tgcgtggctg tatcaacact attatcatca accgttgcag
    10201 gctcccgaaa ttattgcgct ttatgatcaa ggcgatgagc aggcaagggc gcacgttgag
    10261 cgttatctgg atttattagc ggtttgtctg ggaaatatcc tgaccatcgt tgaccctgac
    10321 ctggtcgtca ttggtggtgg cttatcgaat ttcccggcaa tcacaacgca actggcggac
    10381 aggctgcctc gtcatctctt acctgtagct cgtgttccgc gcattgaacg cgcgcgccac
    10441 ggtgatgcgg gaggaatgcg tggtgcggcc ttcctacatc taaccgatta aacaacagag
    10501 gttgctatgc tgtcgcgtcg gggtcatcgg ttaagtcgtt ttcgtaaaaa taaacgccgc
    10561 ctgcgcgagc gtttgcgtca gcgtattttt ttcagagata aagtggtgcc ggaagcaatg
    10621 gaaaaaccaa gagtactcgt actgacaggg gcaggaattt ctgcggaatc aggtattcgt
    10681 acctttcgcg ccgcagatgg cctgtgggaa gaacatcggg ttgaagatgt ggcaacgccg
    10741 gaaggtttcg atcgcgatcc tgaactggtg caagcgtttt ataacgcccg tcgtcgacag
    10801 ctgcagcagc cagaaattca gcctaacgcc gcgcatcttg cgctggctaa actgcaagat
    10861 gctctcggcg atcgcttttt gctggtgacg cagaatatag acaacctgca tgaacgcgca
    10921 ggtaatacca atgtgattca tatgcatggg gaactgctga aagttcgttg ttcacaaagt
    10981 ggtcaggttc tcgactggac cggagacgtt accccagaag ataaatgcca ttgctgccag
    11041 ttcccggcac ccttgcgccc gcacgtagtg tggtttggcg aaatgccact cggcatggat
    11101 gaaatttata tggcgttgtc gatggccgat attttcattg ccattggtac ttcggggcat
    11161 gtttatccgg cggctgggtt tgttcacgaa gcgaaactgc atggcgcgca caccgtggag
    11221 ctgaatcttg aaccaagtca ggtcggtaat gaatttgccg agaaatatta cggcccggca
    11281 agccaggtgg tgccagaatt tgttgaaaag ttgctgaagg gattataaag caggaagcat
    11341 tgcctgatgc gctacgctta tcaggcctac aagcggacca caataccgac gaaagagcgg
    11401 gataaaactt tactgtcctg actcattagc cacatccttg ctaactaaaa aacgggcggt
    11461 aataccaccg cccgcttgct gaattaacgt cctgctttca gcttctgata atactcttca
    11521 taaatgctgc tggctgcgcc aacgtcattc tgccattcgc catttttaat ggtttcagca
    11581 tccgggtaga gcgtcttatc gttcgccact tctggactta acagcttacg cgccgcaagg
    11641 tttggcgttg gataaccgat agtttcagca acctgttttg ccacatccgg gcgcagcagg
    11701 aagttgatca atttcagcgc gccttctttg tttttggcat ttgccgggat cgccaggctg
    11761 tccatccaga atatgccgcc ttctttcggc cacaccacgt caataggcgt acccgcctgg
    11821 cgtgcaacga aagcagaacc gttccagatc atgccgaggt taacttcgcc ttccatgtac
    11881 gggttagccg ggttatcgga gttaaaggct gccacgtttg gcatcagttt tttcagctcg
    11941 ttatatgcag cttcaatctc tttcggatcg gtggtgttac cggagtagcc cagcttacgc
    12001 agcgccatct ggaacacttc acgggcatcg tcggtcagca gcaggctgcc tttgtactca
    12061 ggcttccaca gatcggccca gctggtaacg gatttcggat ctaccgcatc accgttaacg
    12121 ccaatcgccg tcgcccccca gatatacgga atggagtagt cgttattcgg gtcaaacggc
    12181 ttgttgagca tgtctggatc gaggttgctg aaattgctta acttcgactt gtcgatcttc
    12241 tggatcatcc cttctttacg cattttatcg acgtaatagg ttgaaggaac caccaggtcg
    12301 taggcaccgt ctttgtacgt tttcagcttg gcgtacatgg tttcgttcga ctcgtaagtc
    12361 gaatagataa ccttaatacc ggtttctttg gtgaactgtt caagcagtcc tggcggcacg
    12421 tactcggtcc agttgtagaa atacagcgtg ttgttgtcat cggcgtgagc ggcgctcatg
    12481 cccagtgcca gagcacccgc cgcgagcagg tggcgtgacc attttttcat ttaacgtccc
    12541 ctgtgttacc tttcgtttta tcacgagcaa taagctggct ggcaataacc atcaccagcg
    12601 acagcaccag taatatggtt gccagcgcgt taacttccgg cgatacgccg actttgacca
    12661 tcgaataaat ttttaacggc agaatttcat aacttggtcc ggtgacgaac gaagaaacta
    12721 ccacatcgtc catcgacagg gtaaagctta acacccagcc cgccgccacc gctggcattg
    12781 ccagtggcag aatgattttt cgcagaatgg taaattcgct ggcaccgaga tctttcgccg
    12841 cttccagcat tcgcacgtca aaacctttca ggcgcgaata caccgtcacc accacaaatg
    12901 gcaggcagaa ggtgatatgc gagaacagca gcgaccagaa gccaagctga atacccagca
    12961 gcataaacag caccagcagc gaaattgcca tcacgatatc tggtgacatc attaccacaa
    13021 acagcattcc gctaacgaac ggcttaccac gaaagcgata acggtacagt gcaactgccg
    13081 tcagtgaacc gataagcgta gcaaacgtcg ccgaaaacac cgccattgtt agtgaatgct
    13141 gcgctgcctg taacaggctg tcgttgttca tcagcaagct ataccattta gtggtaaaac
    13201 cctgccagtt gatgccaaag cgcgagctgt taaaggagtt cacaatcaaa ataatgattg
    13261 ggatatacag gtacgcgtag atagcggtca taaaaccgcc gcgaagcagt cgaccgatca
    13321 ttcgagttcc accttcttat tcataaggcg ggacactttc aggattttga atttattcaa
    13381 ttttttcctt tctccaaaat ctctccaaaa cttttcccca aaacaggacc acgcatttta
    13441 cagcattttt atacatgggt gagcttacta ggacagaaaa gtattccagt ttgtttaccc
    13501 atatcgcttt ccgaatgaga tttttgatgt gatttcttcg tgttgaaaaa ccacataatg
    13561 tggtattaga attagctatc aagattcact ttgaatccgt tttgaatccc attgaactca
    13621 ggagtgacta tgaagcgtaa aagtgtggct ttgttacaag ccattggctc ggttatagcc
    13681 ctgagcccga cggtcgtttc agcaaatggc atgccagcga atccagtttc cgctttgcgt
    13741 caggatgctg cggctatcag aggcgatttc caaaaagcaa tggtatccgt taacgttgaa
    13801 aagctgaaca atgccagatc aaaaagaaag tgagaacacc aaactcactt gtgaagaaca
    13861 aaaggataat gaactggttt ctcgagtaat cgaaaatcca gaggtcttaa acagggtttt
    13921 ggatagtccg caggtgcggg ctattgtttg ccagcatttt caggggcctg ttccgccacc
    13981 ttcaatgctt aaaaagtatg atcaactggt gcctgggctt gcaaatcgac ttgttgagtt
    14041 gaccgaaaaa gagcaggctc atcgccataa aacagtggct gatagcattg atattgccag
    14101 agatggtcaa acaaaggctt tttggttggc aatattgatc atcttagctg ccactgtctt
    14161 tggcgtcatg ggggagacag ttcttgccgg aactctcgtt tcaatagatc ttgttgcatt
    14221 ggttacggca tttattgttg gaaaacatta ttctaagcag gaacctgatc aagattagtc
    14281 tccgaacccc ggttgatgcc ggggttttta cactagcaaa agcaatatca acatttttca
    14341 gctgcattta tttcatcaaa caaccaattt aacccattcc tgaccacgag tatcgttata
    14401 acgatcggtg gttgcctgga ctttatgtcc cagtaatgtt tttgtatcaa taccctgcgt
    14461 gcggtacagt cgttctgaca gggagcgttg ttcatgaaat gttggcggag tttttcctgc
    14521 tggtggagtt atcccagcca gatcccgtgc tttggcaaag tagtcgctca ggttgtcttt
    14581 actcatcggc tttggttgtt tctggtgccg actatggatt agatatggac ttaatattct
    14641 gtctcggcac ccatcaataa cttcttttaa cgttatccca atggcatcac agcgtagtgt
    14701 aagcggtaac gccagacgca ttccggtttt tccctgggtg atatgcaagt gttcgttcca
    14761 cacatctgaa aaacgcatgt ggcaaatgtc atcacggcgc tgaccagtaa caatcgcaag
    14821 aagcattgcg ttacggataa agtgtttttc aggcgttgca ttgtaaattt tttgccagtc
    14881 ttccatggtg agcctggctc tggttacttt agggatcggt ttacgggtag cctccggagg
    14941 attccatcca ggaggaactt cccctgcatg ctgtgcttct ttataaatat caacccataa
    15001 tccacgattt actctcgctg tgctgaccat gtctttatcc agccactcat ccagtattaa
    15061 tgcaaagtct cttacttcca gttctttcaa tgggtggttt cccagacggg aaaccaggta
    15121 tgcagccatt cgggtttttt ctttgtgagt tgtagctgca atatctccat ttttcagtcg
    15181 cgtgtcctgt attttcagat atcgatcaac ccatgccttt aatctgatac cccgacgttt
    15241 tgttgctgat ggactttcat caattttgcg catgaaatat tctgcttctg ctgcagctat
    15301 tcgctgattg gctgttgaag cgattttttc tgccttgact ttgtctgtcc cgagtccgtg
    15361 aaattttcca gtcacaggat ttttatactg gtagtaaacc ttgccagttc tgcgatcaaa
    15421 cttttcgtaa agagctgcta cgtcagtact gttttttcgt ggcctcggtg acatgggtta
    15481 aaatctcctt cagtgcgtca tcatcgccag tatgaatttc cggcgcaatt ccagcttcac
    15541 ccggtccaac aaagactgcg cggcgatcta ttagccagcg accacgaatt ttttgtggtc
    15601 ttggtacgat gtatccaagt tttccgtatt tcaccagggt agtgtttgtt attgggagac
    15661 tgaaccgctt aggtttccac tcgtcgagcg taatcaggta ctgttcgctc atgaccatca
    15721 ctccggaacg cgccagttgc agaataccaa ccacaaccgg cgacggttga acattaaaaa
    15781 tcagcctgat tcgggaacag tttttgccag ata
//
LOCUS       DAZUGK010000104        15304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZUGK010000104 DAZUGK010000000
VERSION     DAZUGK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.104"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..627
                     /gene="yceJ"
                     /locus_tag="ACHY5W_002839"
     CDS             61..627
                     /gene="yceJ"
                     /locus_tag="ACHY5W_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS4447713.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            631..1206
                     /gene="yceI"
                     /locus_tag="ACHY5W_002840"
     CDS             631..1206
                     /gene="yceI"
                     /locus_tag="ACHY5W_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFS4447714.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1248..2300)
                     /gene="trhO"
                     /locus_tag="ACHY5W_002841"
     CDS             complement(1248..2300)
                     /gene="trhO"
                     /locus_tag="ACHY5W_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4447715.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2525..3445
                     /gene="lpxL"
                     /locus_tag="ACHY5W_002842"
     CDS             2525..3445
                     /gene="lpxL"
                     /locus_tag="ACHY5W_002842"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFS4447716.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3617..4843
                     /gene="mdtG"
                     /locus_tag="ACHY5W_002843"
     CDS             3617..4843
                     /gene="mdtG"
                     /locus_tag="ACHY5W_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFS4447717.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4926..5300
                     /gene="msyB"
                     /locus_tag="ACHY5W_002844"
     CDS             4926..5300
                     /gene="msyB"
                     /locus_tag="ACHY5W_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFS4447718.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLNGEAAQSVFNGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5301..5528)
                     /gene="yceK"
                     /locus_tag="ACHY5W_002845"
     CDS             complement(5301..5528)
                     /gene="yceK"
                     /locus_tag="ACHY5W_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS4447719.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5701..8244)
                     /gene="mdoH"
                     /locus_tag="ACHY5W_002846"
     CDS             complement(5701..8244)
                     /gene="mdoH"
                     /locus_tag="ACHY5W_002846"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFS4447720.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8237..9772)
                     /gene="mdoG"
                     /locus_tag="ACHY5W_002847"
     CDS             complement(8237..9772)
                     /gene="mdoG"
                     /locus_tag="ACHY5W_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFS4447721.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVYVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10166..11323
                     /gene="mdoC"
                     /locus_tag="ACHY5W_002848"
     CDS             10166..11323
                     /gene="mdoC"
                     /locus_tag="ACHY5W_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFS4447722.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPHILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11331..12812)
                     /gene="clsC"
                     /locus_tag="ACHY5W_002849"
     CDS             complement(11331..12812)
                     /gene="clsC"
                     /locus_tag="ACHY5W_002849"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFS4447723.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12754..13287)
                     /gene="ymdB"
                     /locus_tag="ACHY5W_002850"
     CDS             complement(12754..13287)
                     /gene="ymdB"
                     /locus_tag="ACHY5W_002850"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFS4447724.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFTAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13382..13693)
                     /gene="ymdA"
                     /locus_tag="ACHY5W_002851"
     CDS             complement(13382..13693)
                     /gene="ymdA"
                     /locus_tag="ACHY5W_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFS4447725.1"
                     /translation="MFRPFLNSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13792..14145)
                     /gene="csgC"
                     /locus_tag="ACHY5W_002852"
     CDS             complement(13792..14145)
                     /gene="csgC"
                     /locus_tag="ACHY5W_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFS4447726.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISQDDRVKIVVTVSDGQSLHLSQQ
                     WRPLQKSLNLLKYRA"
     gene            complement(14204..14659)
                     /gene="csgA"
                     /locus_tag="ACHY5W_002853"
     CDS             complement(14204..14659)
                     /gene="csgA"
                     /locus_tag="ACHY5W_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFS4447727.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(14700..15155)
                     /gene="csgB"
                     /locus_tag="ACHY5W_002854"
     CDS             complement(14700..15155)
                     /gene="csgB"
                     /locus_tag="ACHY5W_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFS4447728.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAFVAQEGSSNRAKIDQTGDYNLAYIDQAGNANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3799 a         3926 c         3655 g         3924 t
ORIGIN      
        1 tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtttt ggatgcactt
       61 atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta
      121 agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat
      181 tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg
      241 atggggctgg ttattcgcgt tctgtggcgc gtcatatctc caccgccggg tccgctgccg
      301 agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt gtacctattg
      361 ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc
      421 gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt
      481 gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga
      541 tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga
      601 aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc
      661 gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa
      721 ggtcagcacg cctttgttaa cttccgcatc cagcatcttg gctatagctg gttatacggc
      781 acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccggc tgccgataaa
      841 gtgaatgtga caattaacac caccagcgtc gatactaacc acgccgaacg cgataaacat
      901 cttcgcagtg cggattttct caataccgca aaatatccac aggcaacatt cacctccacc
      961 agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct gaatggtgta
     1021 accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc gtggggtggt
     1081 aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca aggacttcaa tatcaagaca
     1141 gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag
     1201 aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga
     1261 tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca
     1321 ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag
     1381 caaatctcac tacaacaacc tttgtatttt tccgcacatg ctggacactg aataaacagc
     1441 aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcacc gcactggtgg
     1501 caatgcgcga taatctcatc agatatacgt tcgcccatcc gctcgtcaaa aacaaaattt
     1561 ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt
     1621 ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt
     1681 tcacaacgaa tgccgccggt gcagtacata acgatttttt tatctttatg tgcctgcatc
     1741 atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc gagcgcgttt
     1801 tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag tgcatcggga
     1861 tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca
     1921 aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc
     1981 cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg
     2041 tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgctggtac gctgatttgc
     2101 gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc
     2161 tgatataaag cgtcacgggt caccttagga tcggcgatgt ggaaatactt ataaaacgaa
     2221 atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc gttggaaatg
     2281 cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg
     2341 gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt
     2401 aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagtc acaaattttg
     2461 gttgcggacg aaaaaatgcg acaatacata caattgcccg tataggttga aaaacaggat
     2521 tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac
     2581 ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccgg ttatctaccg
     2641 cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt
     2701 gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt
     2761 ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg
     2821 gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt
     2881 gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg
     2941 cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc
     3001 actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg
     3061 caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc
     3121 ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg ttgagcaggc
     3181 tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt
     3241 cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc cgccagagtg
     3301 ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa
     3361 atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc
     3421 ggaaggcgtt ccttcacgct attaaatctt ccatgccgga tgcttcagaa tggcatccgg
     3481 cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg
     3541 aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca
     3601 ccaacagcga attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa
     3661 cctgatcgtc gcctggctag gctgttttct taccggtgcc gccttcagtc tggtaatgcc
     3721 cttcttaccc ctctacgttg agcagcttgg cgttaccggc cactccgccc tgaatatgtg
     3781 gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg
     3841 tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgctc tcggcatggg
     3901 catcgtgatg gtgttgatgg ggctggcaca aaatatctgg cagtttttga tcctacgggc
     3961 gcttcttggg ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt
     4021 accgcgtaat aaaagcggct gggcactggg tacgctctcc acaggcggcg ttagcggtgc
     4081 gttgctcggc ccaatggctg gcggcctgct cgccgatagc tacggcttac gtccggtatt
     4141 ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctctttt gcatcagaga
     4201 aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact
     4261 taaaaacccg aaactggtac tcagcctgtt tgtcactacg ttaatcatcc aggtggcgac
     4321 gggctcaata gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa
     4381 cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctgagtgc
     4441 accacgactc ggcaaacttg gcgatcgaat cggccccgaa aagatcctga ttacggcgct
     4501 gatcttttct gtactgctgt tgatcccaat gtcatacgtt cagacgccat tacaacttgg
     4561 gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagaccct
     4621 gttggtttac aactcgagca accaaatcgc cggacgtatc ttcagctata accaatcgtt
     4681 tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg
     4741 tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg
     4801 gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac
     4861 ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa
     4921 tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc
     4981 ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt gcaacagttc aatgcccaaa
     5041 aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaaggtg
     5101 aagaaggtga atgtttacct atgcttaacg gtgaagccgc gcaaagtgtt tttaatggcg
     5161 actatgatga gatagagatc cgccaggagt ggcaggaaga gaatacatta catgaatggg
     5221 acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg
     5281 atgagtggga tgaacgttaa ttactcatac gggccatgat gaatgtcgat cggcagcagt
     5341 aaagtatcaa aaaccaggga gaatggcaga tcaaggattg tgacataacg ccaggcggag
     5401 tcacgcacat cccattgcac accgggataa tattggttgc catgcccctg ccccggtata
     5461 gtgcggctaa tgatgctgcc acagccactc agcaaggtca ccatgatgct caccacaatt
     5521 aaacgcattt gatacctctt ccgtaaaaaa ataccggcat aacgccggta tttttattgg
     5581 ctcgttgttt ttgccagatg cgacgtgaac gccttaccca acctacaaaa tcttgcaaaa
     5641 tcaataaatt gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt
     5701 ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta
     5761 ataactcacc catgaagaat atctctccgg ggaattccag acgcggaaat gcagacgggc
     5821 catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt
     5881 ctcgttcagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct tactggcgcg
     5941 gtgacgcgcg gtcgccattg cggtggccag agcgttaaat gacgggttaa atactgcgtg
     6001 cataaaacca tcatcaagag agcgttgacg gttcatctca aggaaccgat cggtatcaac
     6061 cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc gtttggttcg
     6121 cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga tcaacgagaa
     6181 gacaatcgga gccagccaga acaggaaacg cagatccagc cacgccatcc caacagccca
     6241 aactaaccct aacagcagct gtgagccgtg gcgtttgaac gcttcacccc aggaagtgga
     6301 gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg cgctgacgac
     6361 gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca gcacttccag
     6421 cagcaacgat aatgtaacgc gccagaagcc gccatattct ttcgttcctt tgcaccagat
     6481 aagcaaaatg ctcaataact tcggcaggaa caacagcacc atggtcgaag caaaaagtgc
     6541 aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg gttgcaggaa
     6601 gtattgcggt tcggtcaacg catgtactac ctgcaatgca gtagagagcg cgaggaacat
     6661 aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaataccg cacggtgaac
     6721 cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg
     6781 gcggtcacgt tttagctcat caagcaagtt aggcggtaat tcttcataag aacccgggag
     6841 atcgtaagca atccagactc cccaacctgc acggcgcatc aacgccgctt ccacgaagtc
     6901 atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggggcca gtgcgcagtg
     6961 ctcgataaac ggtttcacgc ggataatcgc gttatgcccc cagtaatgcg actcgccaag
     7021 ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg
     7081 acagcgtgca tacagcgtat ccataccgga cgctttcggc gacgactgaa tgatcccggc
     7141 gttcgggttg gcttccatca agcgcaccag gccgcataaa caatcaccgg tcattaccga
     7201 gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc
     7261 gatattaccg cttttacgct tcacgcggcg acggcggcgg cgatagaaaa tctgaccttc
     7321 gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg
     7381 gttataactg tcactcagaa tgtagacatc aaagtgtttg gcattcccgg tggcttttac
     7441 tgattcccac gttgcacgca agccagcaaa aacacggttc acgtcttcgt tacagatagg
     7501 catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga
     7561 tatactgtat ttatcgcgac caataagcag ttgcaggaag cccattaacg ccgtccagaa
     7621 tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat
     7681 ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat ccataggatt
     7741 aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg cgacgacagt
     7801 ttgcgcgagc gtcaggatca acagaatgta acgccggatg gtaccgacgg tacgccactt
     7861 ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggtg ttacatcgcg
     7921 tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat
     7981 cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg cgcccttcgt catctttaat
     8041 taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc
     8101 ttgcggggag tcatcctccc gcgcccaagt gcggtgttcg gcatccagcg cctgatgaac
     8161 ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc
     8221 aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg
     8281 tctgatcggc attcaccagc gcagcacgca tttcagtggt tttcttggca tctttcactt
     8341 tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc gtgctttcaa
     8401 ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc tctggcagtt
     8461 ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgga
     8521 tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat
     8581 ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct
     8641 ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttggttt
     8701 catcgttggt tggaatttcc accagctcaa cgctgccttt gccccactcc cctttcggag
     8761 tcacccatgc gcttggacgg agatcgtaac gatcatcgag atcttcaaag cgggagaaat
     8821 cacgaccgcg ctgcaacaga ccgaagcctt gcgggttttc catggagaag ctgctgaccg
     8881 ctaaatgttt cgggttattc aacggacgcc agatccattc gccattacca gcatggatag
     8941 agagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg
     9001 gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca
     9061 gatagatttt cgactgcaca tacacaaccg tgtcacgccc tggcataacc acgaatttgt
     9121 aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagtcg
     9181 gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg
     9241 cggtatcaat tgccagaccg cgggcagaaa ggccataaac ctgacctgca ccaatcacgc
     9301 ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg
     9361 ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg tcatgctgaa
     9421 catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca
     9481 cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga
     9541 atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt
     9601 ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct tttaggcgcc tcatagcctt
     9661 tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag
     9721 atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc atttgtagtt
     9781 tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt
     9841 taatggataa ccagccattc cgacaacaga atcccggtat cgttcagcac gctggctcat
     9901 ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca
     9961 tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca gacgcttcca
    10021 ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc gcctggcgcg
    10081 gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga
    10141 aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct
    10201 cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta
    10261 ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttgtggctga ccctttttaa
    10321 tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccgggt acttttccta
    10381 catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg
    10441 gatcccgatg ttaacagcca tccccctact gacattgccg caatttatta tgctgcaata
    10501 cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg tatgacaaat ataatacgtt
    10561 agcctgggaa ttaatatcgc acctgtggtt tttattggtg ttagtggtca tgacgacgct
    10621 gtgcgtctgg atattcaaac gcatcagaaa taatttagaa aattctgata aaacgaataa
    10681 aaaattctcg atggtaaaac tatcggtgat ttttttgtgc ctcggcatcg gctatgcggt
    10741 aataagaaga acgattttta ttgtgtatcc gcacattctg agtaacggca tgttcaattt
    10801 tattgtcatg caaacgctat tttatttgcc gttctttatc ctcggcgcac tggctttcat
    10861 tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc ttgcagcagc
    10921 attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta
    10981 cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg atggtgaatg tggtcttctc
    11041 ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg ttaacgcatc
    11101 gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat acattacacc
    11161 gcacatcacc tccaactggc ttggttttct ctgtggcctg atattcgtag tagggattgc
    11221 gataattctg tatgaaattc atttgcgcat cccgttactg aagtttttgt tctctggtaa
    11281 accggttgtt aagcgtgaga acgataaagc accagcccgt taagccgcgt ttacaataac
    11341 cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa actggtggcg
    11401 ggttcttttt tgagaacaat ctctttcttt gcatgacgat caacccagtt gatccgcccc
    11461 cacctgtcca gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca
    11521 attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaatgtc
    11581 gaacgtggat cgaaattgaa agagccgata aacaccgttt taccatcgat gctaaaggtt
    11641 ttagcatgca ggctggcacc ggaattaccg gttatgccgc gatcgtgtaa cgtactactt
    11701 tgttcacgcg tcggcttgag ttcatataat tccacgccgt agcggagcaa tttttttcgc
    11761 cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga attggttagg
    11821 atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcgcgtgtc
    11881 ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac
    11941 aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca
    12001 cttaataaac gtgttttcgc ccagataagc ggcaacgttc catcaaccag atgatttata
    12061 aacgggctgg attccatttt gcgtaaataa cgatgcgtca tggcatcgtc atgccaggag
    12121 gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac ctgctgtaag
    12181 ggtgaaaccg atttacaata ccagtagcgg gcgaaatcat cggcaacgtc ctctacaact
    12241 ggtcctattg ccatgacatc taaatccgaa aaaagtggct cctcccctgc tccaaaatag
    12301 gcatcaccaa tatttcgccc ccctaccaga gtcaccacgc catcgacagt gaaactttta
    12361 ttgtgcatac ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgtagcaag
    12421 cgaaacgaga aaggattaaa aagccggact tcaatgcggg gatgactgtc aagcaagcgt
    12481 aaaatgtcgt caagtccggg tgtattgttg tcgtccagca acaaacggac gcgaacgcca
    12541 cgctttgctg cggctaacag ggcggaaaac agtaaccgac ccgacatatc gtcctgccag
    12601 atgtaatact gaacatccag tgtatgctct gccatttcgg ccagacgata ccgggcggca
    12661 aacgcatcca gacttttctc cagagggaaa agaccacact gaccgggatg ttgcgaacac
    12721 agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt gggtaaggag
    12781 tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct gttcgggtaa
    12841 agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg atttcagccg ctgccgcacg
    12901 agggtaacca taaaccccag tactgattgc agtaaaagcc actgacgtat agctgtttgc
    12961 cgccaccagt cgtaggctat tgagataggc atcctgcaaa agctggtctt cgttttgttc
    13021 accaccacgc cagactggcc cgacggtgtg cactacggct ttagcgggaa gatcgcctgc
    13081 aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctga cttttaaaca
    13141 ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc
    13201 cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc
    13261 ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg
    13321 tacagcaggc tttttggtta aatcgcattt ttctgctcag caaggtgagt tgttcagaaa
    13381 cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt
    13441 ttactgaggc tatttgcgcg tttttgacat tgccgctcat taacgctttg ctggaatagg
    13501 ttctgcccgg aacagagcca ttctgtgggc agttcatttc tacgggtgat gactgagtgc
    13561 tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc ccttgcgcgg
    13621 tgcttcctgc aatggcaata aaaggaaaaa acatactgcc gagcataaga gagtttaaaa
    13681 aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaggg ttgtgttatc
    13741 catactttca ggttggctga tccgcagcag accattctct ccagattcat cttatgctcg
    13801 atatttcaac aaattaagac ttttctgaag agggcgccat tgttgtgata aatgaagtga
    13861 ctgtccatca gaaacagtaa caactatttt cacccgatcg tcctgggaaa tatttaaact
    13921 caacttcgtc aaagcaatgg gttgattagc aggcaatgag agggtctttt cttgcttcgt
    13981 ctgactttgc cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga
    14041 ttgagtaaga gtgacttcag gaataatggt gtacatatcc ccttgctggg tcgtattaaa
    14101 ggttatctga ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa
    14161 aaaaacaggg cttgcgccct gtttctttaa tacagaggat gtattagtac tgatgagcgg
    14221 tcgcgttgtt accaaagcca acctgagtga cgttgacggt ggaattagaa gcagtctggt
    14281 caaccgctgc accgttgccg ccaccgaact gtttaacagt catttcagaa tttttgccgt
    14341 tccactgatc aagagtggcg ctgttaccga agccacgttg ggtcagatcg attgagctgt
    14401 catctgagcc ctgaccaaca tctgcaccgt taccaccgcc atgctgggta atagtcaagt
    14461 cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt accgccaccg tactggtaaa
    14521 tgttcagctc tgaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc
    14581 cgtactgagg aacaacacct gccagagcgc taccagagaa tacgattgct gcaattgctg
    14641 ctacttttaa aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat
    14701 taacgttgtg tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt
    14761 tgagtaccat actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca
    14821 gtattaccat aagcaccttg cgaaatactg gcatcgttgg cattgcccgc ctgatcaata
    14881 tatgcaaggt tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc
    14941 gcaacaaacg ccaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca
    15001 gcttgaccaa ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag
    15061 ttatattctg aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt
    15121 attgttaaca tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac
    15181 atttaagaaa ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct
    15241 gcgttacgat ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttgtat
    15301 tttt
//
LOCUS       DAZUGK010000105        15261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZUGK010000105 DAZUGK010000000
VERSION     DAZUGK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.106"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..671
                     /locus_tag="ACHY5W_002855"
     CDS             <1..671
                     /locus_tag="ACHY5W_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447729.1"
                     /translation="LLDVFENFESGIPPEDPDLYVFWLAEKQRHPRLFRMEDILGCSY
                     AVILLTQQEFNGPFCEPPELIPNHYRNQQDTPEWMTTGSAFSYFQASVRPKDTPESNF
                     VYRKMGTIPEQSLAFNLAISCKPRAFDPNAGISPTERNNTEYQSIRYFSKDAEGKSKC
                     ETHQWHSAHQPDHLGGSMVPLQSIPDGMSPFYIEFEEYFGGYNFGTGNAWLDFKNMKF
                     DFSC"
     gene            726..1769
                     /locus_tag="ACHY5W_002856"
     CDS             726..1769
                     /locus_tag="ACHY5W_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFS4447730.1"
                     /translation="MTFKHFLLSLVSGLLLISSHNVAAEKILRYTDHEPYGGMRTQII
                     KEIFFAEIEKESQGRLKIEPHWNGETAISYDALTTISDGSKADMGIVVPEYTAKQLPL
                     HQIFKSFAIGPDHGASQVEFFRRVYAEIPEFNAELERNNIVNLQFFLGYPVGFFSTRP
                     IDKLTALQGTTWRTASFWHRAYLTHTGAKTVTLPWNEQITTALRDGQLDGLMVNLDSG
                     YDIHAERAAPNVLLSPSLWLGHVYLLVMNKQTWENLDNRDREAIQRAAITTEKALGKA
                     LDNNLISMVKTLEQEGAQVRYLKKSELDAWQKAIGYQQEQTQWVEKQNNEGVEKAGGV
                     MQKVANILDETMR"
     gene            complement(1811..2686)
                     /gene="mscS"
                     /locus_tag="ACHY5W_002857"
     CDS             complement(1811..2686)
                     /gene="mscS"
                     /locus_tag="ACHY5W_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS4447731.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKENKENEAVTR"
     gene            complement(2877..3956)
                     /gene="fbaA"
                     /locus_tag="ACHY5W_002858"
     CDS             complement(2877..3956)
                     /gene="fbaA"
                     /locus_tag="ACHY5W_002858"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4447732.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4171..5334)
                     /gene="pgk"
                     /locus_tag="ACHY5W_002859"
     CDS             complement(4171..5334)
                     /gene="pgk"
                     /locus_tag="ACHY5W_002859"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4447733.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5384..6403)
                     /gene="epd"
                     /locus_tag="ACHY5W_002860"
     CDS             complement(5384..6403)
                     /gene="epd"
                     /locus_tag="ACHY5W_002860"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFS4447734.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6775..7206
                     /locus_tag="ACHY5W_002861"
     CDS             6775..7206
                     /locus_tag="ACHY5W_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFS4447735.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7229..7807
                     /locus_tag="ACHY5W_002862"
     CDS             7229..7807
                     /locus_tag="ACHY5W_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4447736.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            7808..8515
                     /locus_tag="ACHY5W_002863"
     CDS             7808..8515
                     /locus_tag="ACHY5W_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS4447737.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TIILLMLTEFGAWIWLR"
     gene            8503..9180
                     /locus_tag="ACHY5W_002864"
     CDS             8503..9180
                     /locus_tag="ACHY5W_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4447738.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9174..9851
                     /locus_tag="ACHY5W_002865"
     CDS             9174..9851
                     /locus_tag="ACHY5W_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4447739.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFENWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRIIN
                     PPDDIHP"
     gene            complement(9823..10536)
                     /locus_tag="ACHY5W_002866"
     CDS             complement(9823..10536)
                     /locus_tag="ACHY5W_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFS4447740.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10533..11042)
                     /gene="fumE"
                     /locus_tag="ACHY5W_002867"
     CDS             complement(10533..11042)
                     /gene="fumE"
                     /locus_tag="ACHY5W_002867"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFS4447741.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EAPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(11064..12029)
                     /gene="glpX"
                     /locus_tag="ACHY5W_002868"
     CDS             complement(11064..12029)
                     /gene="glpX"
                     /locus_tag="ACHY5W_002868"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS4447742.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(12026..13303)
                     /gene="yggP"
                     /locus_tag="ACHY5W_002869"
     CDS             complement(12026..13303)
                     /gene="yggP"
                     /locus_tag="ACHY5W_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS4447743.1"
                     /translation="MKTKVVAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(13318..14706)
                     /gene="cmtA"
                     /locus_tag="ACHY5W_002870"
     CDS             complement(13318..14706)
                     /gene="cmtA"
                     /locus_tag="ACHY5W_002870"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS4447744.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(14734..15177)
                     /gene="cmtB"
                     /locus_tag="ACHY5W_002871"
     CDS             complement(14734..15177)
                     /gene="cmtB"
                     /locus_tag="ACHY5W_002871"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFS4447745.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNNISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
BASE COUNT         3769 a         4041 c         3682 g         3769 t
ORIGIN      
        1 aattattgga tgtgtttgag aattttgagt ccggaatacc ccccgaagat ccagaccttt
       61 atgttttctg gctggcagaa aagcaacgac atccacggct atttcggatg gaagatatat
      121 taggttgttc ttatgcggtg attttactca cccagcaaga gttcaacggc ccattttgtg
      181 aaccgcctga acttatacct aatcactatc gcaaccagca agatacacca gaatggatga
      241 ccaccggcag tgcgtttagt tattttcagg caagcgtcag accaaaagat acgcctgaaa
      301 gcaattttgt atatcgtaaa atgggcacca ttcctgagca gtcgctggct ttcaatctgg
      361 cgatttcatg taagccgcgc gcttttgatc ctaatgcggg aattagcccc acagaaagaa
      421 ataatacgga atatcagtcc attcgttatt tttctaaaga tgcagaggga aagagtaaat
      481 gtgaaactca ccagtggcat agtgcacatc aacccgatca tctcggtggt tcaatggttc
      541 cattgcaatc tataccagat ggaatgtcac ctttttatat tgaatttgaa gaatacttcg
      601 gcgggtataa ttttggtact ggcaatgcgt ggctggactt taaaaatatg aagttcgatt
      661 ttagttgtta aagttcacgg cttttaatat taatgacgac caataaaatt acagatggaa
      721 ctgagatgac attcaaacac tttttacttt ctctggtatc aggattactt ctgatatctt
      781 cccacaacgt tgccgctgaa aaaattcttc gttatacaga ccatgaaccc tacgggggaa
      841 tgcgtacaca aataattaaa gagatatttt ttgcagaaat tgaaaaagag tcgcaggggc
      901 gtttgaaaat cgaaccacac tggaacggtg aaaccgccat cagctatgac gctctgacga
      961 caataagtga tggtagcaaa gctgatatgg gtatagtggt accggaatac acagcgaaac
     1021 aattaccgct tcatcaaatc ttcaagagct ttgctattgg cccggatcat ggagccagtc
     1081 aggtagaatt ctttcgtcgc gtatatgcgg aaattcccga atttaacgct gaacttgagc
     1141 gtaacaatat cgtgaattta cagtttttcc ttggctaccc ggtaggcttt ttctctacca
     1201 ggcccattga taaattgact gcacttcagg gaaccacctg gcgaacagcc agtttctggc
     1261 atcgggctta tttaactcat acgggggcaa aaaccgtaac cttaccgtgg aatgagcaaa
     1321 ttacgacagc actccgcgac ggacaactcg atggcctaat ggtcaatctt gatagcggat
     1381 atgacatcca tgctgaacgt gctgcgccga atgtgttgct ctccccttcg ctctggcttg
     1441 gtcatgttta tctgttggta atgaataaac aaacgtggga aaaccttgat aacagagatc
     1501 gtgaggctat tcaacgagcg gccattacaa ccgagaaagc gctgggcaag gcattagata
     1561 acaacctgat cagcatggta aaaacgcttg agcaggaagg tgcacaggtt cgctatttga
     1621 aaaaatcaga gctggacgcc tggcagaaag cgattggtta tcagcaagag caaactcagt
     1681 gggtagaaaa gcaaaataat gaaggcgtgg agaaagccgg gggagtcatg caaaaagttg
     1741 ccaatatact tgatgaaaca atgcgttaaa ttggtagtgc gcccatgaac ttcatgggcg
     1801 ctttacatga ttaacgagtt acggcttcgt tttctttatt ttctttcacc cgcttaaagt
     1861 tcacatccat ttgcgggtac gggaagctga taccggcggc atcaaattca cgtttaatac
     1921 gctccagcac atcccagtac acgttttgca gatcgccgct gttgctccag acgcggacca
     1981 cgaaattaat cgacgatgca ccaagttcgt tcaggcgcac agtcatttcg cgatctttca
     2041 aaatgcgatc ttcagactgg ataatattgg tcaggatctg cttaacctga tcgatatcgg
     2101 aatcatacgc cacgccaata ataaattcgt tacggcgaac tggctcgcgg gagaagttaa
     2161 taatatttcc ggcaataatt ttaccgttcg gaataacgat aattttaccg tctgcagtac
     2221 gcatggtggt ggagaaaatc tgcacactca gcacagtacc ggctacgccg ccgaggtcaa
     2281 catattctcc ggcacggaac gggcggaaca tgacaagtaa cacgccagcg gccaggttag
     2341 acagtgaccc ctgcaaagcc aaaccaacag ctaagcctgc ggcaccgagt acagcaatga
     2401 cggacgcggt ttgtacaccc acgcgtccca gtgcagcgat tagcgtaaag gcgataatac
     2461 cgtaacggac taatgcagaa agaaaatcag caacagtggc atcgatttta cgggagatca
     2521 tcaggcgatt caccgcgttg gaaatcatcc gcgcgataat caaaccaacg atgatgatcg
     2581 cgagtgccgc cacgatgttt actgcataac ttagcagcag cgcctggtta gccaccagcc
     2641 agcttcccgc gccgtttatg ctatcgacaa cattcaaatc ttccattcaa tattcctttt
     2701 caactgactc aaaatggaga aatacactcc gccgccttat gacgggcagt ctgacaaggg
     2761 taaacaataa agcataattt tgccaaatag atcacagatt tctcatctgc atcaccttgc
     2821 ttttggcgaa aaaaagaccc gcagagcggg ccttgagata agcagaaagg aatgacttac
     2881 agaacgtcga tcgcgttcag ttcctggaat gctttttcca gacgagcgat catcgaagtc
     2941 tgaccggcac gcagccatac gcgcggatcg tagtatttct tgttcggctg atcttcgcct
     3001 ttcgggttac ccagctgacc ctgcagataa gcttcgttcg ctttgtagta gttcagaacg
     3061 ccttcccagg ttgcccattg ggtatcggta tcgatgttca ttttcactac gccgtagctt
     3121 acggagtctt tgatttcctg agcagtagaa ccggaaccac cgtggaatac gaagttcagg
     3181 ctgttgtgcg gcaggttgtg tttcttagaa acatattcct gagaatcacg caggatggtc
     3241 ggagtcagaa ccacgttacc cggcttgtaa acaccgtgta cgttaccgaa ggacgctgcg
     3301 atggtgaaac gcgggctgat tttgctcagc tcggtgtatg cgtaatcaac gtcttccggc
     3361 tgggtgtaca gtgcagaagc gtccatgtgg ctgttgtcca cgccgtcttc ttcaccaccg
     3421 gtgcaaccca gttcgatttc cagagtcatg ccgattttgg acatgcgctc caggtattta
     3481 gagcagattt cgatgttctc ttgcagagat tcttcagaca ggtcgatcat gtgagaagag
     3541 aacagcggct tgccggtagc agcgaagtgt ttttcacccg cgtccagcag accgtcgatc
     3601 cacggcagca gtttcttcgc gcagtggtca gtgtgcagga taaccggaac accataatgt
     3661 tcagccatct ggtgaacgtg atgcgcacca gagatcgcgc ccaggatagc agcaccctgc
     3721 ggaacgtcag atttcacgcc tttaccagcg ataaaagaag caccaccgtt ggagaactga
     3781 acgataaccg gcgctttaac tttagcagcg gtttccagta cggcgttgat ggagtcagta
     3841 ccgacgcagt ttactgctgg cagtgcgaag ttgttttctt ttgctacctg gaaaactttc
     3901 tgtacgtcat caccagtgat tacgccaggt tttacgaaat caaaaatctt agacatgtct
     3961 cttgtcctgt atcgtcgggc cttatactcg tcatacttca agttgcatgt gctgcgtctg
     4021 cgctcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc tcttgtcgcc
     4081 ttcctgcaac tcgaattatt tagagtatga aaaaatgcgt gctctaaaag cgcgctgaaa
     4141 caagggcagg tttccctgcc ctgtgatttt ttacttctta gcgcgctctt cgagcatcgc
     4201 tactgcaggc agtactttac cttccacgaa ttcgaggaat gcgccgccgc cagtggagat
     4261 gtaggagatt ttgtcagcaa tgccgaacag gtcgattgct gccagagtgt cgccgccgcc
     4321 agcgatggag aacgcttcgc tgtctgcgat agcgttagcc acgatttcag tacctttgcg
     4381 gaagttcggg aattcgaaca cgccaaccgg accgttccac agaatggttt tcgcattctt
     4441 caggatttca gccagttcct gagcggaagc atcaccgata tccaggatct gctcgtcagc
     4501 tttcacatcg ttaacagatt tcagggtagc cggtgcagtt tcagagaact cggttgctac
     4561 gcgaacatca gacggaaccg ggatgttgca ggtggtcagc agacgttttg cttcgtcaac
     4621 caggtcagct tcgtacaggg atttacccac atcgtggcct tgtgccgcga taaaggtgtt
     4681 agcgatacca ccaccaacaa tcagctggtc agcgatttta gacagggagt ccagaacggt
     4741 cagtttggta gatactttag aaccaccaac gatagccacc atcgggcgag caggttcttt
     4801 cagtgcttta cccagcgcgt ccagttcagc tgccagcagc gggcctgcgc acgcaacgtc
     4861 agcgaattta ccgataccgt gagtagaagc ctgcgcgcgg tgagcagtac cgaatgcgtc
     4921 cattacgaac acgtcacaca gtgcagcgta ttttttggac agggtttcgt cgtctttctt
     4981 ctcgcctttg ttgaagcgaa cgttttccag aacaaccagt tcaccttcag caacgtcaac
     5041 gccgtcgagg taatctttaa ccagacgaac cgggttagac agtttgtctt tcaggtagtt
     5101 aacaaccggc agcagagaga attcttcgtt gtactcgcct tcggtaggac gacccaggtg
     5161 ggaagttacc atcacttttg cgccttgttt cagggccagt tcgatggtcg gcagagaagc
     5221 acggatacgc gcgtcgctgg ttactttccc gtcttttact ggtacgttca gatccgcacg
     5281 gataaataca cgtttcccag caagatccag atcggtcatc ttaattacag acatggtgaa
     5341 tcctctcgtt gattctaaaa gttttgcaga cgctgcttgc gtcttacctg aaagcaacag
     5401 tagccatagc taacgtcgtg tcgagcattc ggttagcaaa gccccattcg ttatcgcacc
     5461 agaccaacgt tttgatcagg tgtgcgccac tgacccgggt ttgggtgcca tcgacaatgg
     5521 cactgtgcgg atcgtggtta aaatctacag agaccaacgg caattccgta tagtcaacta
     5581 taccatgaaa tgcaccttgt gctgcttttt gcagcaacag gttgacttca ttggctttta
     5641 caggtttctt caccgtcacg cttaaatcga ttgccgtcac atttatggtt ggcacacgta
     5701 ccgcaatcgc ttcaaagcga tcgttaaatt gcggaaaaaa tcgtgtgata ccggcggcca
     5761 gtttagtatc gaccggaatg atcgactggc tggctgcccg ggtgcgacgc aggtcaggat
     5821 ggtatgcatc aataacctgt tgatcgtgca tggcggagtg aattgtggtc acagtgccgg
     5881 actcaatacc gtacgcatca tctaacaatt tgatgacggg aattatgcaa ttcgtggtac
     5941 aggaagcgtt agaaacgatg cggtgttccg cacgaagttg atcctgattg acgccgtaaa
     6001 caacggtcgc gtcgagatcg ttactgccag gatgtgaaaa gagcactttt ttggccccgg
     6061 cggcaatatg cgcttcgcca tgctcgcggg agccatatac gccggtgcag tcgagtacta
     6121 catcaacgcc aagttcacgc caggggagcg attgcagtga acgttcatgc aatacgcgga
     6181 tggcgtcatc accgacaaaa agttgatcgc gttcctgtcg tacttcccat gcaaaacggc
     6241 catggctggt gtcatatttc aacaaatgcg ccatgcccgc agcatccgcc agttcgttga
     6301 ttgccaccac ggtaatttcc gcccggcgtc cggattcata caaagcacga accacattac
     6361 gcccgatgcg accgaagcca tttatcgcta cgcgtacggt catagatctc ctgcaaggtt
     6421 ttccctgagc aaatttgcca gacagagtaa tccagcaaat cgtccgggga aaccttacct
     6481 gtcgcaaact gcgactgatt ggttaattgt cgaacattta atcgactgaa acgcttcagc
     6541 taggataagc gaaacgtgga ataaaaggaa tgattgtcca gtcaaagaaa acaattatct
     6601 gacttacgtc acgttttcag ccccgcccct gtggaattta ttccactttg cagaatcacg
     6661 aataagcgta gcaaaatctt ttcgttgtgc cgtcacaaac cctgacttat atcagaatat
     6721 gactgatgca gcgtcgatat tatccgcaat acgtccgagt ttcggagtac tccaatgatg
     6781 acattgccct acccatattc ttcctccgca cgcttttacg cactgcgcct cctccccggg
     6841 caggaagttt tttcccagct acacgctttt gcacagcagc agcaacttca tgccgcctgg
     6901 attgcaggct gtaccggtag cctgactgac atcgccttgc gctatgccgg gcaagaaggc
     6961 accacgctcc tgaacggtac attcgaagtg atctcactaa acggcacgtt ggaacagagt
     7021 ggtgagcatc ttcatctctg cgtttccgat ccacacggca cgatgcttgg cggtcatatg
     7081 atgcccggct gtaccgtgcg gaccacgctt gaactggtga tcggtagcct tgaggaactg
     7141 gcgtttagcc gccaaccttg cgcgctttct ggttacgacg agttgcatat ttcccccgtt
     7201 aaataactac tttcagagag gttttcttat ggcgcgtagc cacttttcca gtcaggcact
     7261 ggtgttgatc gttatatcta tcgccatcaa tatgatcggc gggcaactgg ccagcatggt
     7321 caaattacct atatttctcg actccatcgg cacacttatt agcgcggtac tgcttggtcc
     7381 ggtgatcgga atgctgaccg gcttactgac caatttactc tggggattgc tgaccgaccc
     7441 gatcgccgcc gcatttgcac ccgtcgcgat ggtcatcggc ctggtcgccg gttggctggc
     7501 gcgagcgggt tggtttcgca ccctgccaaa agtcgttgtc agcggcgtga ttattacgct
     7561 tgcggtaacg gtcgtcgctg taccgctgcg tactgcgctg ttcggcggtg tcaccggtag
     7621 cggagccgat ttgttcgttg cctggatgca ctctatgggg caaaacctgg tggagtcggt
     7681 ggcgattacg gtgataggcg caaacctggt cgataaaatt cttacagcgg tgattgtctg
     7741 gcttttactg cgccagctgc cgattcgcac cacgcgccat tttccggcaa tggctgccgt
     7801 gcgctaaatg catccgttta catccttaac gctgtgggcg ctggcggcct gcaccacgct
     7861 tatcctgccc gcgcagacca ttttgccgat ctatagcgcg gcgacctttt tttgccttat
     7921 tgcgctaaag gccacgcggc ggcgggcaaa atatgtcgtc tggctgatgt tctcgctggg
     7981 agccgggctg tggctggtac acggtggctg gctgacagaa tggctaagcg gcacgccacg
     8041 ctcgcctgaa cggtggacgc atgctattac gctctggttg cgcattctgg ctatcgtttc
     8101 aacctcacag ttgtggatgc agtacgtccc cgtacagcgc tttatccgcg ccctgttcgc
     8161 ctcccgcctg ccgccgggcg tcgcttatct gtttgccggg ccgttattgg tcgttgagca
     8221 attaaaacgg caattagcga ttattcatga agcacagcgt gcgcgcggcg tgccgctgga
     8281 tgagggctgg tatcagcgtt tacgtgcgat gcccgcgtta attattccgc taacacacaa
     8341 tgcgctaaac gatcttgctg tccgcggtgc ggcgctggat atgcgggcat ttcggatcca
     8401 caaccgacga acaacgttgt gggcaccagc cgatagcacc ctacaacgcg tagcgcgtta
     8461 caccattatc ttgctgatgc tgacagaatt cggagcgtgg atatggttac gttagaacag
     8521 ttccgttatt gcccgacaca ctcaacgcat cctccgttct gttatgactt tcactatgtc
     8581 aaaccgggga tggtcgccat ctttggtgac aacggcagtg gcaaaagtac gctggcacag
     8641 ctgatggcgg gctggtatcc ggactttctg ccaggagaga taacaggcac ggggacgctc
     8701 ctcggtacgc ccattgggca tctcccgctt aatgaacaat ccgccaccat ccagctcgtc
     8761 cagcaatcgc cctatttgca gctctccggc tgtaccttta gcgtggaaga agaagttgcc
     8821 tttgggccag aaaatctgtg tcttgctgaa aaagagatta tggcgaggat tgatgccgcc
     8881 ctgactctga ccgagtgcca gccgttgcgc catcgtcacc cggcaacact ctctggtgga
     8941 gagacacagc gcgtggtcat cgcctgcgcc atcgcgatgc agccaaaact attaattctt
     9001 gatgaagcct ttagccgcct gacgccgcag gccagcgaaa tgctgttgca gcgcttacaa
     9061 cactgggcgt ttgaacgtgg ttcgctgatt attctttttg aacgtcatcg cacacctttc
     9121 ctgaactact gccagcaagc gtggcaattg caaaacggag cgctccagcc actatgttaa
     9181 cgttaaatca gatatcttat cgctggcctg gcgcggcaac ggattgtctt tgcgacattt
     9241 ctctgcaact caaacaaggt gaatggttgg cgttgaccgg tgacaatggc gcagggaaat
     9301 cgacgctgtt acgagtaatg gcaggtcttc ttacgcccac agccggtacg gttatgctgc
     9361 aacaacaggc gatgaaaaac ctgaaaaacc gccagcgcgc ggcaaagatt ggcgtactgt
     9421 ttcaggaagc ggaaaaccaa ctttttcaca gcaccgtcgc tgacgagatc gcatttggtt
     9481 tgaaactgca aaaatgcccg gcggatgaaa tcactcaacg tacccatgct gccctacaat
     9541 gttgtcagtt agcagacact gccagcgcgc acccgttgga tctgcatagt gcgcagcgac
     9601 ggatggtcgc cgtcgcctgc ctggaagcac tctctccgcc actattgctg ctggacgagc
     9661 cgagtcggga ttttgacgaa aattggttga gcgtgtttga aaactggctg gagaagtgcc
     9721 gccagcgagg cacgagcgtc gtagcaatca gtcatgacgc cgcatttacc cggcgtcatt
     9781 tctctcgtgt ggtgcgactg gaagatggtc taatcaggat cattaatcca ccagacgata
     9841 ttcacccgta gcggtcatca ttaaggtcaa atttgcaggc agactctctt ccagcacccg
     9901 gcgaacattc ggcccgtcgg tacggtcata aaaagcttct gcgtctgcca gcgataaccc
     9961 tcccgccagc ttgcgcccca ccagtcgttc ccgcaacgca gtagctggcg cattgataaa
    10021 gatcgagaaa tcacaaaatt gcgcaagctg gcaccacttc tcgtcgtcca gcaacaacca
    10081 gtttccttca acgatgacga gtggtgcggt aacgtgcaac gcatcttcaa caggatcatg
    10141 cttttgtcga tcatactgcg gccacgtaca atccccttcc acgacctggc gcagattttc
    10201 cgccagtttc gctacgtcaa atgtctctgg tgcgcctttg aaggggcgca attgatgcgc
    10261 atccagccag ctattgtaat ggtgaaaacc atccatcggc agcgtctgaa tagcgggcag
    10321 ttccgggtct tgttgcgcga gatattccca gaaggtggtt agggtagatt tacccgtccc
    10381 cggcggagcg caaagaaaaa ccacggttcg gcgctgcgga tttaccgtct gtaaggccgc
    10441 cagcatacgc agcaacggtt tatgcacgcg ttctatctct tcatcatggt actgcgcttg
    10501 cacgttcagc ccattcaccg ttaattcaat tttcacggcg ttaattcctt taaggtggtc
    10561 gttactgcca gcgtcagcgc cgttttgacc attccgctaa aacgcgcctc tgaccagacg
    10621 gcgaaatcgg cgaacttcat cgctttcaac gcgtcataga gcatcgcatt gcgggttatg
    10681 ctattgacgt tgctgataaa atcccaggtc atgtcatcgg caaatccggg cgcttcatcc
    10741 tgttcgttca ggtaatgcca gaactgggaa aaatgagtga tatcggcgta cagccattta
    10801 tccattttcc ccagcgcata aatcaaacgc agcgcaacat caatatcgtc aagcgggccg
    10861 ctttgggcaa gtaacggctt cactgcatat tccacgatct cttcatcgtt atcgacaaac
    10921 agcgacggca gaaactggcg tattccctgg agcaaaatag aatgcgcagt tttcataaat
    10981 gaaaataaat tgtcctgtgc atccagttgc tcaagcacat catcttctgt cagcgtcgcc
    11041 attggtcttc cgatagatca ccatcaatgc agggagtcta ttatattaca cgtttggtcc
    11101 gcgccgccga tcagtaatgt ctgcgtccgc accccattcg ccgtctgttg gataccgttc
    11161 accagctcgc cgcccgtgac gcccgtagcg ctaaagagga tatcgttccc cctcaccagt
    11221 tcgtcgagcg agtacacacg gttaacgtca acgcccattg ccttgcaacg cttgcgctcc
    11281 tgctctgcaa tctgccgatt ttccgtgtaa tcccctttag cctgacaaaa atcgattaat
    11341 tccgcctgca tatctccgcc taacgctttg actgcacagg cagaaatcac gccttccggc
    11401 gcgccgccga tggtgtacat cacgtcgtaa ggattatcct gccagcaggt cagcacgctg
    11461 gcggcgacat cgccatccgg cagggcaaaa actttcaccc ccagctgtgt tgcttcctca
    11521 atcgcggcac tcaggcgcgg tttatccagc gtaaccatgc gcagcttgtc cagcggttta
    11581 cctaacgccc gcgccacgtt acgcaagttg tccgccagag gcaaagacag atcaatcgcc
    11641 ccggcagcca gtcgattaac aaccagcttt ttcatataca tatcgggcgc atgtaacagg
    11701 ctatctcgag gggcgaaagc catgaccgcc agcgcattgc tctgccccat cgccaccatc
    11761 cgcgtgcctt caatgggatc aaccgcgata tcgacttctg gcccatcgcc tttgcccacc
    11821 tcttcgccaa tccacagcat cggcgcatgg tcaatttctc cttcgccgat aaccactcgt
    11881 ccacgaaaag cgacgtcgtt taatgcctgg cgcatcgcgg tgaccgccag gccatcaatt
    11941 ttatttttgt cgccacatcc ggtttgcggc caggcagcca acgctgcctg ttccgtaacg
    12001 cgaaataatg gccacgccag ggacatcatt gcgcggcctc cccgatatca acgccaaatt
    12061 gtgccagcag atatttctcg gcctgttcat tccagatgcc gtgcgtttcc tcaaccagcc
    12121 gcgccaactc tttaaacagc ggatcggttt tgcctttttc ggcaaaatcg gcaatggcag
    12181 tgagcggcat ggtcacgccg ttataaatga gttttttacc gccagggata tccggcagat
    12241 tgagcacggt ttctggcacc gcatccaggc caccgatatg ggtcaccata aacgacggct
    12301 gtaactgccc agtggcgcta agggcaatcg cctctttcat gtcgtccgtt gaaccgccgg
    12361 atgtaccgac gacgtgcgtg ctgttgtaat gaacgttgta gaaattaaac ggcactttga
    12421 agtttttatc cgtcggcccg gcaaagaagt tcagacagcc atcttccgcc agtaattcat
    12481 cagccatctc aacgacagca ggcaccgccg cataaacaaa aatgtcatcg aacccggcat
    12541 ctcctgtcag cgcccgcagc atctggacag gatcgctcat ccctttggta ttcacataca
    12601 ccagctcaat gcctttactg gccgccagtt ccaccggcag cagcttctgt acctgcgcca
    12661 gacgtttgtc gtcgatatcg accaccacca cccgcgacgg ttgtatgccg ccgttaatgg
    12721 cgtaatcgat agcgccaatg cccatcggac ctgcacacgc cagcagtgca atattgccgc
    12781 caggtttgac gcccatgcga tgctcataaa catattgcgt ggtgtgataa ttggcatgat
    12841 aagcaccaat aatgcagcac ataggctctg ccagcgacgc cgcagcaaaa taagagccgt
    12901 gatacggtaa tacgcagccc aaattaatgg cgatttcggg aataatcata taagtggcat
    12961 tgccgccaaa atattcgtag ctgtagcccg ctgaatatcc gcttggtaac cccatcgccg
    13021 gttgcaatac aaaacgctgg ccttttttat atttgccagt gagattttta cccacttcga
    13081 caataacccc ggcacattca tgcccggtaa tgaccggatg attttctaaa tcgtcgggta
    13141 cgcgtttatg ttcactaccg agtaacgccg ctttccaggt ggataaacag acgctgtcag
    13201 aaatcacact caccagtaat tcattatcgg taatttctgg cagttcaaat tcgcgcagac
    13261 ggacatcccg cttgccataa atagcaacaa ctttggtttt catttttacc tctgtaatca
    13321 gtgtttatgt tcggcggtta attgattaaa taaagtgtcg agttttggat cgccaatata
    13381 gttattaatc aatatcagtg gtttatccgt cacacgtttc acgcgccctt ccagactggc
    13441 gtgagtgacg acaatatccg catccgcagg cacgttttct atagcgtaat gtttaacctc
    13501 tattgccagc ccggcttttt ccagacgttt gcggaaggtg gtcgcgccca tcgcactcga
    13561 tcccatcccc gcatcgcaaa caaaggcaat acgcttaacc ctgcttaacg agaatgcacc
    13621 ttcttgtttc atcgccttaa ccgcattggc tgactgagca aactcatctt cgctctccgt
    13681 ttccaccgtt ttttccatct tcagtatcag cgaagtaata gcaaaggaca ccagggtacc
    13741 taccgtaaca ccggcaattg tcgccaggaa cgagcctttc ggcgtcagtg ccaggtaagc
    13801 aaagatagac cccggacttg ggccagccac cagaccaccg tccagtaagt taaacatcca
    13861 ggtgccagac ataccgcccg caatcatggc aataatagtc agcggcttca tcagcacata
    13921 tgggaaatac agttcgtgga tcccaccgag gaagtgaata atcatcgccc cgggcgcaga
    13981 acgtttactc atccctttac caaacaaggt aaacgccagc agcagcccca ggcccggacc
    14041 tgggttagag gccaccataa agaagatgga tttaccgtta accgaagcct gttgcattcc
    14101 cagcggataa tagacgccct gatcgatcgc attattaagg aaaagcactt tcgccggttc
    14161 attgatgact gacagcagag gcagataacc cgcatgtacc agcgcctcaa tacactcttt
    14221 gacgaaagta ttggcaatta acaccgccgg gccgatcact tcaaaaccca gcagacaaag
    14281 gagcatcccc gcgataccta atgagaagtt attgataacc atctcaaaac cggcaggtat
    14341 gcgtttttcc agtgccttat caacatattt tatgaccagc ccaccgagcg gccccataat
    14401 cattgagcca aggaacatcg ggatctctgc gccgacgatc acaccaatag tacctattcc
    14461 gcccatgacc gcgccgcgtt taccgccgac cagatgacca ccggtagaac caatcatcac
    14521 gggcaataaa taggtaatca tcgggccgac aattttggcg aaatgttcgt taggcagcca
    14581 accggtggga ataaataacg cagtaataaa accccaggca ataaaagcac caatattggg
    14641 gatgaccatt gcagtcaaaa agcccccaaa agcctggacc tttgcacgag cagacttgtt
    14701 ttccataata ttatcctata gaggagagag taattatccg cggctgataa tgtccgctaa
    14761 ttgtttttct gatgatgctg ttaagagttg ttcgagtatt tcttcttcgc acagtaattc
    14821 acttaacgcc tgaatagcgc caatgtgcga atcggcatcg gcagcagaga gtccgatgag
    14881 taatttaatt ggttcgtcat tccccggaaa ataaacacct tgttcaagta atgtcaaaga
    14941 catcccggtt ttaagcgccc cacattccgg tcgcgcatga ggcattgcca cgcctggtgc
    15001 gagaatataa taagggccat tgttaatggt ggaatcttta attgcctgaa tgtaattctc
    15061 gctgatgttg tttttatcca gcaatgatac catcgagaaa tcgatagctt cctgccaatc
    15121 ttttgctgaa tgtataactg agattgatga ttctggaaaa taatcactaa gccgcataaa
    15181 ttatccttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
    15241 aaagcaattg ggtacgcgag a
//
LOCUS       DAZUGK010000106        15241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZUGK010000106 DAZUGK010000000
VERSION     DAZUGK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.107"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            209..481
                     /locus_tag="ACHY5W_002872"
     CDS             209..481
                     /locus_tag="ACHY5W_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFS4447746.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(482..1354)
                     /gene="rluF"
                     /locus_tag="ACHY5W_002873"
     CDS             complement(482..1354)
                     /gene="rluF"
                     /locus_tag="ACHY5W_002873"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFS4447747.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPAGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLNGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKVEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1702..2649
                     /locus_tag="ACHY5W_002874"
                     /pseudo
     CDS             1702..2649
                     /locus_tag="ACHY5W_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            2719..3525
                     /locus_tag="ACHY5W_002875"
     CDS             2719..3525
                     /locus_tag="ACHY5W_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4447748.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTAAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3535..3942
                     /locus_tag="ACHY5W_002876"
     CDS             3535..3942
                     /locus_tag="ACHY5W_002876"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFS4447749.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3942..4437
                     /locus_tag="ACHY5W_002877"
                     /pseudo
     CDS             3942..4437
                     /locus_tag="ACHY5W_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4503..5300
                     /locus_tag="ACHY5W_002878"
     CDS             4503..5300
                     /locus_tag="ACHY5W_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4447750.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMHVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5311..6135
                     /locus_tag="ACHY5W_002879"
     CDS             5311..6135
                     /locus_tag="ACHY5W_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4447751.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGFLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6187..7416
                     /gene="sorE"
                     /locus_tag="ACHY5W_002880"
     CDS             6187..7416
                     /gene="sorE"
                     /locus_tag="ACHY5W_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFS4447752.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILAAVVTDSLCLSSWK
                     EANLGENHKKVPEDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYALTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILTRHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7796..8485
                     /gene="pepE"
                     /locus_tag="ACHY5W_002881"
     CDS             7796..8485
                     /gene="pepE"
                     /locus_tag="ACHY5W_002881"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFS4447753.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8576..10207)
                     /locus_tag="ACHY5W_002882"
     CDS             complement(8576..10207)
                     /locus_tag="ACHY5W_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFS4447754.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10427..14110)
                     /gene="metH"
                     /locus_tag="ACHY5W_002883"
     CDS             complement(10427..14110)
                     /gene="metH"
                     /locus_tag="ACHY5W_002883"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4447755.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLKLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVIKVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALAESFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLDVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14310..15134
                     /gene="iclR"
                     /locus_tag="ACHY5W_002884"
     CDS             14310..15134
                     /gene="iclR"
                     /locus_tag="ACHY5W_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFS4447756.1"
                     /translation="MVAPIPAKRGRKPAAATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3629 a         3992 c         3999 g         3621 t
ORIGIN      
        1 gataaaaaat tttaattaca tgaaaaataa agatttaata aatcattgtt gtttgtaggg
       61 ggagattttt tcgctaaatt tgtatctgta tttatcgtca attatcaaga aagataaata
      121 tttaagaaga gaacaaagga atattatcag ttctgcttgc aaataaattc ccgtacactg
      181 aactatccga ttaacagagg gcaccatcat ggcactcccc cgtattactc aaaaagagat
      241 gactgagcgt gaacagcgcg aacttaaaac actgctggat cgggcacgga ttgcacatgg
      301 tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg ataaattaat
      361 ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta aagaaaaagc aggcttataa
      421 accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg
      481 attagcgccc cttcttttta cgccccggcg aggtaaaacg cttaccgttg gacgccgggc
      541 gaccgccttt ttccgccgtt ttttctacct tcaccaccgg gcgtttgatg cctgccgttt
      601 tcggtttggc cttcgctttg ggcttcgcct cggaagagga attttcaatg agcttaaaga
      661 ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc tagtggaatg ccgtttaagc
      721 tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccaaag tgttcgcaca
      781 tgcgacggat ctgacggttc agcccctgca ccagggtaat gcggaagaca aacggagctt
      841 cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcactccc gcccccatac
      901 cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct ttctcatgat
      961 cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg aaaatcagcc
     1021 cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat
     1081 cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccggcg ggcttgttca
     1141 gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg tttactttca
     1201 caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta aggaacacat
     1261 tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt
     1321 atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa tgcggaatat
     1381 accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc
     1441 tgccacgcct tttatcacaa aagtgtctcc ggaagtacaa tgcaaatttg cctggcaaaa
     1501 taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta ctgcgcagag
     1561 aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa aatcgctttg
     1621 aaaggtcatc atgatggggt tatatcagat caagcggtga gaaagcgtat ttgcacaaat
     1681 gtgcaaaatc aggaggcggc aatggaaaac agtgacgata tccgtttgat tgtgaagatt
     1741 gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt
     1801 taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc
     1861 gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa gcaaaagttt
     1921 ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac tcaactggcg
     1981 atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg cgatattgtc
     2041 ggtttttcct agggtcgcgc ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa
     2101 tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact cgaaagccgc
     2161 tatcacgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc
     2221 gcggattttc cggctctact ggataaccca ttaattcgta atgggatcat gcagtttcag
     2281 cactttaaaa ccatctctgc ctactgggat aatctggatg tcgccctggt gggaattggc
     2341 tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga agagagtgac
     2401 gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga tattcacggc
     2461 gcaatggttg aaacgaatat gagcgaaaaa acactctcta tcgaaatgaa taaattaaag
     2521 caggcgcggt attccattgg cattgccatg agtgaagaaa aatacagcgg aattgttggt
     2581 gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc tgaactatta
     2641 ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt ccctattatc
     2701 taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat cattatcgtc
     2761 accggcggcg catccggtat tggtctggcg attgtagagg aattattagc acaaggcgca
     2821 aacgtacaga tggtagatat tcacggtggc gatggtcaat atgaagttca taaaggctat
     2881 cagttttggc cgaccgatat ttccagcgcc aaagaggtaa atcatacggc agcggaaatt
     2941 atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt
     3001 ctgctggtcg atgagaaagc gcctgccggg cagtatgaac ttaacgaagc tgcattcgaa
     3061 aaaatggtca atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag
     3121 atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg actggaaggc
     3181 tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc
     3241 tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg
     3301 gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac gcgcaatatc
     3361 accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga
     3421 agattagcag aagtcgctga ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc
     3481 accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc
     3541 aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg
     3601 gtttacggca acgccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac
     3661 atcgtagtaa agctggaaaa gttagtaagc attcacaatc aagatgagtg gctgattgcc
     3721 gtggatttac agtgcggcag cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct
     3781 cgtctgcggg taatcagcgg actttctctg ccgctggcgc tcgaactggt tgataaccag
     3841 gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc
     3901 gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt acgctcgccc
     3961 gcattgatga ccgcctgatc cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg
     4021 cccagcgcat tattatctgc aatgacgaag tttataacga tgaagttcgc cggacattat
     4081 tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccatcg
     4141 cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac
     4201 aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctaaat attggcggca
     4261 tggcctggcg gcccggtaaa aaaacagtta accaaagcgg tttcattaga tgatgatgat
     4321 attaacgcat ttcatgagtt aaataatctt ggcatcatac tggatttacg cgtcgtagcg
     4381 tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc aaattaaata
     4441 atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa aggtgacaaa
     4501 tcatggaaat aagtacccta caaattattg ctatatttct tttttcctgt attgccggaa
     4561 tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt acggtgattg
     4621 gtttaattct cggtgattta aaaaccggaa taatactcgg tggtacgctg gaattgatag
     4681 ctctcggctg gatgcacgtc ggcgcggcgc aatctccgga ctctgcactc gccagcataa
     4741 tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc gccatcgcgt
     4801 tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc accgtagtgt
     4861 tccagcacgc ggcggataaa gcagcggaag aggcgcggtt tcgcactctc gatattctgc
     4921 atgtctccgc gcttggcgtg caggcgctgc gcgttgctat tccggcactg attgtctcgc
     4981 tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa tttgtgaccc
     5041 gaggtctgca aattgctggc ggttttatcg tggtggtcgg ttacgccatg gtgctacgta
     5101 tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca ggtggctacc
     5161 tcgatctcag tctgctggcg ttcggtggcg tcggcgtgat catggccctg gtctacatcc
     5221 agctaaatcc acagtggcgt aaagctgaac ctcaactcca ggccaccgcc tccaccgccc
     5281 ttgaccaact tgatgattaa cggagccatc atggaacaga gaaaaattac acgcagcgat
     5341 ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt tgaacgtatt
     5401 cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta cccattaaaa
     5461 gaggatcagg ttgcggcgct caggcgacac ctggtgttct tcaataccac gccagccgta
     5521 tgtggcccgg tcatcggcgt cactgctgcc atggaagagg cgcgggccaa cggcgcggaa
     5581 attgatgacg gcaccattaa cggcatcaaa gtcggtctga tgggaccatt ggcaggggtt
     5641 ggcgatccac tggtctgggg aacgctgcgc ccgattaccg ccgcgctcgg cgcatctctg
     5701 gcactttcgg gcaacattct cggcccgctg ctgttcttct ttattttcaa cgcggtgcgt
     5761 ctggcgatga agtggtatgg cctacagctc ggctttcgca aaggggtgaa tatcgtcagc
     5821 gatatgggcg ggaatgtgct gcaaaaactc accgaaggcg cgtcgattct cggactgttt
     5881 gtgatgggcg tgctggtcac caaatggacg tcaatcaacg taccgttggt ggtttcacaa
     5941 acgcatgccg ccgatggctc caccgtcatc atgaccgtgc agaacattct cgaccaactt
     6001 tgccctggtt ttctggcgct cggtctgacg ctactaatgg ttcgtctgct caacaaaaaa
     6061 attaacccgg tatggctgat tttcgccctg tttggcttag ggattatcgg caatgcgctg
     6121 ggcttcctgt cctgattctt cgccccggca cgactgccgg ggccatcgct caacatgagg
     6181 tggtttatga aaacaacagc tctgcgtctt tatggtaaac gtgatttacg cctggaaacc
     6241 tttgaccttc ctgaaatgca ggaggatgaa atcctcgcgg cggtggtcac tgacagcctg
     6301 tgcctctctt cctggaaaga ggccaacctg ggtgaaaacc ataaaaaagt acccgaagat
     6361 gtggcgacca acccaatcat catcggccac gagttttgcg gcgatattct ggccgtgggt
     6421 aaaaagtggc agcacaaatt ccagccgggt cagcgttatg tgattcaggc caacctgcaa
     6481 ctccccgacc gcccggactg ccccggctac tccttcccgt gggtaggcgg cgaagccacg
     6541 catgtggtta ttcccaacga ggtcatggaa caagattgcc tgcttgcata cgacggcgaa
     6601 acctattttg aaggctcgct ggttgaaccg ctttcctgcg tgattggcgc gttcaacgcc
     6661 aactatcatc ttcaggaagg tagttataac cacacgatgg ggattcgccc gcaagggcgc
     6721 acgctgatcc tcggcggcac cggaccaatg ggactgttgg cgattgatta tgcgctacat
     6781 ggacccgtta acccgtcgct gctcgtcatt accgataccg acaacgataa attgagttat
     6841 gcgcgcaagc actatccatc agaaccgcaa acactgattc attatctcaa tgccaccgat
     6901 gcagcatttg atacgttaat ggcgctgagt ggcggtcacg gcttcgatga tattttcgtc
     6961 tttgtgccta atgaaggact ggtgactctc gcctcttcct tgctggcgac agatggttgc
     7021 ctgaatttct tcgccggacc gcaggataaa catttcagcg cgccaattaa tttctacgat
     7081 gtgcattatg cattgaccca ctacgtgggc acgtcaggcg gcaataccga cgacatgcgc
     7141 gcggcggtca aactgatcga agagaaaaaa gtgcaggccg caaaagtggt aacacatatt
     7201 cttgggctga atgccgcggg cgaaaccacg cttgaattgc ctgccgtcgg cggcggcaaa
     7261 aagctggtgt ataccgggaa atacctgccg ctgacgtcac tcacgcagat tcaggatcaa
     7321 gcactggcgg cgattctgac gcgtcatcag gggatctggt ccggtgaggc ggagcaatac
     7381 ctgctcgccc atgcagaggc aatttcccat gattaatcgc gctactcaac tctgcatgtc
     7441 gctggctggt cgccccggca acttcgcgac cggatgtgcg gctgattgct gagcggccca
     7501 atgtgcacga gaaggataaa aaatatcagc gccttaaggg gcgctcaaat tggtggcaaa
     7561 gtacacattg tttatgccgg atgcgggatg aaatcttatt cggcctacat aatctcgaaa
     7621 attcaatata ttgcagttat ctggtaggcc aggtaagcat agcgcatcag gcaatgctgt
     7681 gtttgtcatc agtctcagcg cctgtgcaat acgtgacatc gtgagccatg tcagtaagtg
     7741 gttaaattcg cagtggtagc atgccttccg tgatttctta ctaaccggag aagtaatgga
     7801 actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac atgcactgcc
     7861 gctgattgct gaacagttgc agggtcgccg ctcagcggtg tttatccctt tcgctggcgt
     7921 aacgcagacc tgggatgatt acacagcgaa aacggctgcg gttcttgctc cgctgggtgt
     7981 ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa atgctgagat
     8041 cgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg agcgcgggct
     8101 gctggcacca attactgacg tggttaaacg tggcgcactg tatattggct ggagcgcagg
     8161 cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg tcgatccgca
     8221 aggtttcgat gcgctgaatc tgttcccgtt gcaaatcaac ccgcacttca ccaacgcgct
     8281 gccggaaggc cataaaggtg aaacccgtga acagcgtatt cgcgaactgc tggtcgtcgc
     8341 gccagaactg acgattattg gtctaccgga aggtaactgg atcacagtga gtaaaggtca
     8401 cgctacgctg ggtggcccga acaccactta tgtgtttaag gccggtgaag aagcggttcc
     8461 gctggaagcg ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg catcttgttc
     8521 atagtgcctg atgcgacgct tgacgcgtct tatcaggcct acaggattca caatattaat
     8581 actcgtcccg tccttcatct tcatccggct gttccagcac actgtaagcc accgaacaaa
     8641 acagcgagtt cagacgctgc atatctccca gtaagcctaa atgtagcgaa ctggtttcaa
     8701 tgctctgcac gttttgctga tgcaggcgat cgacgtgggc gtgggaatag cggcgattaa
     8761 gaatgcgaaa gcgatgcttg ctgcggcgca aacgacgggc gctggtaaca tcaccagaga
     8821 agaaaaccga cattgccagt tttaaattac tgagcaattg ctcatagagc gcatccagtt
     8881 ccttcaagcc atcaagcgaa aatgcccgcc gtgctgccag cgatttatcg gcaatttcgc
     8941 tccccatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt tcgatgatct
     9001 ccgcccagcg gcgcgactct tcctccgcca gctcttcttt tggcatccgc gccagataca
     9061 gcttaatggc ggtatagaga acgttgatat catctgccag cttacgcagc tctttctcct
     9121 gccgtggctc gccgtgcatc actttattca gcccttccat catctgttcc atggcgtcgc
     9181 caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgta tccagcgcgc
     9241 tgacatccag atgtttgggc cgtagctggg tatccagttc cggttcatcg cgaataatcg
     9301 ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat gaccaggcag cgtacaaggt
     9361 tgtagaagac gtggaaatag atcaccagtt ccgcttttgg caatgacagc ttccccattg
     9421 tctctgccag caaatggaca aacggcagga taatcaggct acccaccagc ttaaacagca
     9481 gactacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg agcatcgcca
     9541 gcaggccgga accgaggtta gcaccaatca ccagacagag tgccacgggg aaggagataa
     9601 tgcctgcggc ggtcagagtc gcggtcagca gtacagcagc aaggctggag tagctgataa
     9661 tggcgaacat cgcgccaatc agcgcatcca gcagaatatc gccggtcagc gaggcaaaga
     9721 tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc aactccagcg
     9781 ccagcaaaat cagcccaaga ccaataccga cgcggcccag ttgcccggcg cgtgactgtt
     9841 tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa cggtgacagc caggataagt
     9901 cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcccccagc acaatgacca
     9961 gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc atggtggtgg
    10021 cattactgct ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg agcggctttt
    10081 tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc attacgccgg
    10141 ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg tgaagcagcg
    10201 ttaacacgag ttcaggttct ccttatcgtt atatgttcct gaagcaattg aatgaccaaa
    10261 ggaccggaaa cacgctgacg ggcatcttta ctgtgccttt tgacattcgt ttttccggct
    10321 aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag acagggcact
    10381 cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag tccgcgtcat
    10441 accccagatt cggtgccagc cagcgctcaa catctgagac gctcatacct ttacggcggg
    10501 catagtcttc aacctgatcg cgctgaattt gcgccacggc gtaatacttg ctgtccgggt
    10561 ggctgaaata ccaacctgaa accgacgctc caggccacat cgcgaaagat tccgtaagtt
    10621 tcatgccggt gtgtttttct acgtccagca gctcccagat ggtggctttt tccgtatgtt
    10681 ccgggcaggc cgggtagcct ggtgccggac ggatgccttg atagttttcg cggatcagct
    10741 cttcgttgct gaggttctcg tttggtgcat agccccagta aactttacgc acgcgctcat
    10801 gaagatattc cgcaaaggct tcggctaaac ggtcggcaag ggctttgacc atgattttgt
    10861 tgtaatcatc gtgctgtgct tcgaacgatt ctgccagcgc gtcctcttcc agcccgccag
    10921 tcacagcaaa tgcgccgatg taatccgctt taccggagag cttcggtgcc acgaagtcag
    10981 cgagacagta gttagcgaag ccggtttttt ccgtctgttg acgcagatgg tggctgactt
    11041 tgatcacatg ggtgcgcgtt tcatcacggt agatttcaat gtcatcaccc acacggtttg
    11101 ccgggaacag ccccaccacg ccgcgtggat tcagcgtttt ctcggcgctt aatttatcca
    11161 gcatgtcgtt ggcgtcttta aacaggcgct gcgcctcaac gccaaccact tcatcttcca
    11221 gaatgcgcgg atacttcccg gccagcgacc aggtcataaa gaacggcgtc cagtcgatgt
    11281 aattacgcag cgtttcgatg ctggcttcaa cttcctgcac gccgagacgg tgcgccaccg
    11341 gtggcgtgta agcctgccag tcaaaagcga aatcgttatc gcgcgccgct tccagcgtga
    11401 ctggcggtgt gcgcggtttc ttgcgcccgt gctgaatacg cacggtttcg tactctttgc
    11461 gggtacgagc aacgaaatca tcgcgctgag tgtcggaaag cagcgccgcc accacaccaa
    11521 cggtgcgcga ggcattctgc acatacaccg tcgggccgct gtagttctgc tcaattttca
    11581 ccgccgtgtg cgcttttgag gtcgtcgcgc caccgatcag taacggaatg gtgaagccct
    11641 gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg ataagccccg
    11701 aaagtccaat cagatcagca ttcacttctt tagcggtacg gagaattttt tccgcaggca
    11761 ccataacgcc gagatcgaca atttcgtagt tgttacattg cagcaccaca ccaacgatat
    11821 ttttaccgat gtcgtggacg tcgcccttca cggtggcgat caccatcttg ccgttggttt
    11881 tgccctgctc tttgctggct tcaataaacg gttcgaggta ggccaccgcc tgcttcatca
    11941 cacgcgccga tttgaccacc tgtggcagga acattttccc ttcgccgaac agatcgccga
    12001 ccacgttcat gccgtccatt agcgggcctt caatcacttc aatcggacgc gtagcctgct
    12061 ggcgtgcttc ttcggtatcc tgctcgataa actcggtaat gcctttgacc agcgagtatt
    12121 ctagacgttt attcacttcc cacgagcgcc actccgcctg ctgggcgtta gcggtgtcgt
    12181 cggttttgct gccgcgatat ttctcggcaa gctccagtaa acgctcagtg ccatcatcgc
    12241 gacgattaag aatcacatct tccaccgcgt cgcgcagttc agcgggcagg tcgtcgtaaa
    12301 tcgccagttg cccagcgttg acgatcccca tatccatgcc attgcgaata gcgtagtaga
    12361 ggaacaccgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag aaagaaacgt
    12421 tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct tcgcaggcgc
    12481 cgataaagtc ctgcgcatag ttgttgtgct cttcaatgcc ggtggcgacc gcgaagatgt
    12541 tcgggtcgaa gatgatatct tctggcggga agccaacctc ttcggtgagg attttgtacg
    12601 cccgacggca aatctcgatt ttccgtgcgc gggtatcggc ctgtccctgt tcgtcaaagg
    12661 ccattaccac caccgctgca ccgtaacgac gcaacagttt cgcatgatgg ataaaggcgt
    12721 cgacgccctc tttcattgag atagagttaa caatgccttt gccctgaata cacttcagcc
    12781 ccttttcgat gacctcccat tttgaggagt cgatcataat cggcacgcga gcgatatccg
    12841 gttcaccggc aatcagtttg agaaaacgca ccatcgccgc ttcggcatcg agcatccctt
    12901 catccatgtt gatatcgata atctgcgcac cgttttccac ctgctggcgg gccacatcca
    12961 gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcggaa ccggtgacgt
    13021 tagtccgctc gccgacgttc acaaacaggc tgtcgtcgcc aatgttcagc ggctccaggc
    13081 cggacaaacg gcaggctacg ggaatttcag gcagtttgcg cggcgctaat ccttctactg
    13141 cacgactcat cgctgcaata tgttgtggcg tggtgccaca gcagccgccg acgatattga
    13201 gaaaacccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg tcgagatcgt
    13261 actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc ggtgacgtag cattccgcaa
    13321 tccgtgacag ctcctgcacg tactggcgca gttcatctgg ccccagcgca cagttcaggc
    13381 caaaggtcag cgcttcggcg tggcgcaatg agttgtaaaa tgcttcggtg gtctgcccgg
    13441 agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc agctcaacgc
    13501 ccagcgcttc aaactccgtt ttcaccgcaa aaaccgccgc tttggcgtta agggtgtcga
    13561 aaacggtttc aatcaggatc agatccgcac caccttccac cagcgctttg gtggattcgc
    13621 gataggccgc caccagctgg tcaaaagtga tgttgcggaa tgccggatcg ttgacgtccg
    13681 gagaaataga cgccgtgcgg ttggtcgggc cgagaacacc ggcaacgtag cgcggtttct
    13741 ctggcgtgcg cgcggtccac tcatcagcac aggcgcgcgc cagtttcgcc gccgcaaagt
    13801 tgatttccgc cgacagggat tccatctggt aatccgccat cgcaatggtc gtggagttga
    13861 aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg atagcggcga
    13921 tcacttccgg tttactgagt accagcaggt cgttgttgcc tttgaggtcg catggccagt
    13981 cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg atcatggtgc
    14041 ccataccgcc gtccagcacc agaatacgtt catttaactg cgcacgcagt tgttccactt
    14101 tgctgctcac acttgctccc gacacgctca acccagattt aataaaaatt caacaaacca
    14161 tactggcata aacgcatctg tggtaaaagc gaccaccacg caacatgaga tttgttcaac
    14221 attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa atgaaaatga
    14281 tttccacgat acagaaaaag gagactgtca tggtcgcacc cattcccgcg aaacgcggca
    14341 gaaaacccgc cgctgccacc gcccccgcta ccgggcaagt acagtcttta acgcgtggcc
    14401 tgaaattact ggaatggata gccgaatcca atggcagtgt ggcactcaca gagctggcgc
    14461 aacaagccgg gttacccaac tccacgaccc accgcctgct aaccacgatg caacagcagg
    14521 gtttcgtgcg tcaggtcggc gaactaggac attgggcaat cggcgcacat gcctttatgg
    14581 tcggcagcag ctttctccag agccgtaatt tgttagcgat tgttcaccct atcctgcgca
    14641 atctaatgga agaatctggt gaaacggtca atatggcggt gcttgatcaa agcgatcacg
    14701 aagcgattat tatcgaccag gtacagtgta cgcatctgat gcgaatgtcc gcgcctatcg
    14761 gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc caactgagcg
    14821 aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc cacgcaacgc
    14881 tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg ggttattcat
    14941 ttgacgatga ggaacatgca ctggggctac gttgccttgc agcgtgtatt ttcgatgagc
    15001 accgtgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt accgatgacc
    15061 gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg ctggcgtacg
    15121 gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccatc atacctgagt
    15181 ggcaatagaa taagggtgtc tgttaatcgc attgacgcca aaataactta atgtcataca
    15241 c
//
LOCUS       DAZUGK010000107        14942 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZUGK010000107 DAZUGK010000000
VERSION     DAZUGK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.108"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..178)
                     /locus_tag="ACHY5W_002885"
     CDS             complement(<1..178)
                     /locus_tag="ACHY5W_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447757.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKY"
     gene            complement(409..1383)
                     /gene="yajO"
                     /locus_tag="ACHY5W_002886"
     CDS             complement(409..1383)
                     /gene="yajO"
                     /locus_tag="ACHY5W_002886"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFS4447758.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEDLGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1438..3300)
                     /gene="dxs"
                     /locus_tag="ACHY5W_002887"
     CDS             complement(1438..3300)
                     /gene="dxs"
                     /locus_tag="ACHY5W_002887"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4447759.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTQLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3325..4224)
                     /gene="ispA"
                     /locus_tag="ACHY5W_002888"
     CDS             complement(3325..4224)
                     /gene="ispA"
                     /locus_tag="ACHY5W_002888"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFS4447760.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRNRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(4224..4466)
                     /gene="xseB"
                     /locus_tag="ACHY5W_002889"
     CDS             complement(4224..4466)
                     /gene="xseB"
                     /locus_tag="ACHY5W_002889"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4447761.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4672..6120
                     /gene="thiI"
                     /locus_tag="ACHY5W_002890"
     CDS             4672..6120
                     /gene="thiI"
                     /locus_tag="ACHY5W_002890"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4447762.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(6174..6764)
                     /gene="yajL"
                     /locus_tag="ACHY5W_002891"
     CDS             complement(6174..6764)
                     /gene="yajL"
                     /locus_tag="ACHY5W_002891"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFS4447763.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6727..7638)
                     /gene="panE"
                     /locus_tag="ACHY5W_002892"
     CDS             complement(6727..7638)
                     /gene="panE"
                     /locus_tag="ACHY5W_002892"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4447764.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7806..8297
                     /gene="yajQ"
                     /locus_tag="ACHY5W_002893"
     CDS             7806..8297
                     /gene="yajQ"
                     /locus_tag="ACHY5W_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFS4447765.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8425..9789)
                     /gene="yajR"
                     /locus_tag="ACHY5W_002894"
     CDS             complement(8425..9789)
                     /gene="yajR"
                     /locus_tag="ACHY5W_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4447766.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(9938..10828)
                     /gene="cyoE"
                     /locus_tag="ACHY5W_002895"
     CDS             complement(9938..10828)
                     /gene="cyoE"
                     /locus_tag="ACHY5W_002895"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFS4447767.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(10840..11169)
                     /gene="cyoD"
                     /locus_tag="ACHY5W_002896"
     CDS             complement(10840..11169)
                     /gene="cyoD"
                     /locus_tag="ACHY5W_002896"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFS4447768.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11169..11783)
                     /gene="cyoC"
                     /locus_tag="ACHY5W_002897"
     CDS             complement(11169..11783)
                     /gene="cyoC"
                     /locus_tag="ACHY5W_002897"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFS4447769.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11773..13764)
                     /gene="cyoB"
                     /locus_tag="ACHY5W_002898"
     CDS             complement(11773..13764)
                     /gene="cyoB"
                     /locus_tag="ACHY5W_002898"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFS4447770.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13786..14733)
                     /gene="cyoA"
                     /locus_tag="ACHY5W_002899"
     CDS             complement(13786..14733)
                     /gene="cyoA"
                     /locus_tag="ACHY5W_002899"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFS4447771.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3729 a         3931 c         3814 g         3468 t
ORIGIN      
        1 tgtatttatg aatcgattta aatgcctttt gtttatcact gtccagaaaa aaatcacgaa
       61 ttttgttcca cagcctttca ataagatttt gatttaaatc ttgcttcatt gagtttctta
      121 aatctgcaac cggtacatca gtaaacagta tcggttgttt ggtgatggtt ttcatcatga
      181 ttgtaatcct cttacattga ctaaataaga catttttagg ggatattatc gggagaaact
      241 atcaaaaatc gctcaaagcg atgaagatga tgaatagctg taaatttaat cacgaagaat
      301 ttctcttgca gaggtggatt atggttacag aaggtgatgg gaggtacgtt gtccccatca
      361 tcgcagacga tggggaattc agcagataaa atgggcgaaa agcgaaattt acttaaatcc
      421 tacgaccgga tgcggtttat acggcgtttc cagttcggca atctgttctg gcttcaaagt
      481 gatatccacc gcgttcagta gctcatcaag ctgttcttcc cgcgatgtac cgataatcgg
      541 tgcagcaatg ccaggtttgc tcaacaacca tgccagtgcg acttgtgctc gtgtcgcgcc
      601 cagatcttca ctgacgcccg ttaaccgctc cgcaatttgc gcgtcattct catcgctttc
      661 tttatagaga tttttcccca cctcatcaga caccagtcgt gcagtggttt ctccccacgg
      721 acgcgtcaga cgtccccgtg ccagcgggct ccacggaatc accgccacgc cttcctgata
      781 acacagcggc agcatctcgc gctcttcttc gcgataaatc agattgtagt gatcctgcat
      841 actgacaaac tgcgcccagc cgtgctgttt ttgtagctcc agcgcctgag caaactgcga
      901 agcgtgcatt gatgacgcgc cgatataacg cgctttcccg gcttttacca cgtcgttgag
      961 ggcttccagc gtctcttcga tcggcgtgtt gtaatcccag cgatgaattt gcaggatatc
     1021 gacataatcc atgccgagac gtcgcaggct gtcgtcgata gagcgcaaaa tttgtgcacg
     1081 ggataatcct tccggtaaat caccaacgcg atggaacact ttggtggcaa cgaccacgtc
     1141 ttcacgacgg gagaaatccc gcagtgcgcg accgacgatc tcttcgctgc tgccatcgga
     1201 atagctattg gcggtatcaa agaaatttat gccgccttcc agcgcgcgtt taattatggg
     1261 acggctgctc tcttccggca gtgtccatgc gtgattacca cgatcaggct cgccaaaggt
     1321 catacagccg aggcaaagtc gggaaacgcg aaggtcggtt tttcctaagg ggttgtattg
     1381 catgctgcca ctcctgccat gaataatttc ttaagcatag caggagtgga gtagggatta
     1441 tgccagccag gccttgattt tggcttccat accggcggca tcgaggccga gttcggcgcg
     1501 catttcttcc tgagttcctt gcggaataaa gaagtccggc aggccaatgt tcagcacggg
     1561 tactggttta cgatgggcca tcagcacttc gttcactccg ctgcctgcac cgcccataat
     1621 ggcgttttct tctacggtaa ccagtgcttc atgactggcg gccatttcca gaattaacgc
     1681 ttcatcaagc ggtttcacaa aacgcatatc gaccagcgta gcgttcagcg attcggcgac
     1741 tttcgccgct tctggcatca gcgtaccaaa gttaaggatc gccagtttct cgccacgacg
     1801 cttcacaatg cctttgccaa ttggtagttt ttccagctgc gtcagttcca cgccaaccgc
     1861 gttgccgcgc gggtagcgca ccgctgacgg gccatcgtta tagtgatagc cggtatagag
     1921 catctggcga cattcgtttt catcgctcgg ggtcataatg accatttccg gtatgcagcg
     1981 caggtaagag agatcaaaag cgccctggtg ggtttgaccg tcagcaccaa caatgcccgc
     2041 gcggtcgatg gcaaacagga ccggaagctt ttgaattgcc acgtcatgca gcacctgatc
     2101 ataggcgcgt tgcaggaaag tggagtaaat cgcgacaatg ggtttgtacc caccaatggc
     2161 cagacccgca gcaaaggtca ctgcgtgttg ctcggcaatc gctacatcaa aatagcgatc
     2221 cgggaatttg cgtgaaaact cgaccatacc ggaaccttca cgcatcgccg gagtaatcgc
     2281 catcagcttg ttgtctttcg ctgccgtttc gcacaaccag tcgccaaaga tttttgaata
     2341 gctcggcaaa ccgccgctac ttttcggcaa acaaccgctg gagggatcaa atttaggcac
     2401 ggcgtggaag gtgattgggt ctttttctgc cggttcataa ccacgacctt ttttggtcat
     2461 gatatgcagg aactgcgggc ctttcaggtc gcgcatgttc ttaagcgtgg tgataagccc
     2521 cagcacatcg tgaccgtcaa ccgggccgat gtagttaaag cccagctctt caaacaacgt
     2581 gccaggcact accatgcctt taatatgttc ttcggtacgt ttgagcagct ctttaatggg
     2641 cggaacgcca gagaaaactt ttttcccgcc ttcgcgcagt gaagagtaaa gcttaccgga
     2701 aagcagctgc gccagatggt tgttgagcgc gccaacattt tcggaaatcg acatttcatt
     2761 gtcgttgagg accaccagca tatcaggacg gatatcgccc gcgtgattca tcgcttcaaa
     2821 cgccatgcct gcggtaatcg cgccatcgcc aatgacacag acggtacggc gatttttgcc
     2881 ttctttctcg gcagcaaccg caataccaat tccggcactg atggaggttg atgaatgccc
     2941 gacgcttaat acgtcatatt cgctttcgcc gcgccacggg aacgggtgca gaccaccttt
     3001 ttgacggatg gtgccgattt tgtcgcggcg tccggtcaaa attttatgcg gataagcctg
     3061 atgccccaca tcccaaatca attggtcaaa cggggtgttg tagacatagt gcagcgccac
     3121 ggtcagttcg accgtgccca gcccggaggc gaagtgcccg ctggaacggc tcacgctgtc
     3181 gagtaaatag cggcgcagtt cgtcgcagag tttcggtaaa ctctctttcg gcaacagtcg
     3241 taactcctgg gtggagtcga ccagtgccag ggtcgggtat ttggcaatat caaaactcat
     3301 caggggccta ttaatactta ttgtttattt attacgctgg atgatgtagt ccgctagcgc
     3361 ttccagtgcc gaggtatcga gtgactgttc agccagttgt ttcagcgact gacgggcatc
     3421 gtcgatcaga tcccgggctt tcttccgggc ttgctcaaga cccagaagtg cagggtaggt
     3481 acttttacca agttgctggt cggcaccctg gcgttttccc aacgttgcag tatctcccac
     3541 cacatccagg atgtcatcct gaacctggaa ggcaaggccg atgctctctg catacttgtc
     3601 gagtactggc agagcacgac gtcctttatc tccggcgctt aatgcaccaa ggcgaacggc
     3661 ggcgcgaatc aatgcgccgg ttttatgacg atgaatacgc tcaagcgcgt ccagaggtac
     3721 gtgtttgcct tccgcgtcta aatctaatgc ctgaccaccg cacattccgg caataccact
     3781 agcgcttgcc agttctgaaa tcatcgaaat tctgttgcgg tccgacactt ccggcatatc
     3841 ggcatcgctt aaaatcgaga acgccaacgt ttgtaaagcg tcaccagcga gaatcgcgtt
     3901 tgcttcgcca aacttcacat ggcaggtcgg caaaccgcga cgcagatcgt catcatccat
     3961 cgccggtaaa tcatcatgaa ttaatgagta agcgtggata cactctacgg cggcagcggg
     4021 tgcgtccagc gtgtttgtgc taacgccaaa catatgaccg gtggcataaa ccaggaaagg
     4081 tcgcaggcgc ttaccaccta ataatgcgcc atactgcatg gtttcgacca cgggagtgtt
     4141 ctgaaagggc agtggagcga taaaacggct cagcgcctgg ttggcctgct taacgcaggc
     4201 ttcgagttgc tgcggaaagt ccattactca ttgtccggtg taaaaggggt tagagaggcg
     4261 tcttcattgt cagacagcag aatttgtacg cgctgttcgg cttgttgtaa tttggcctgc
     4321 ccctgacgtg ccagctgcac gccgcgttcg aactcgttca gcgcctcttc cagcggcagg
     4381 tcgccacttt ccagacgggt tacaatctgt tccagctcgc tcagcgcctt ttcaaagctg
     4441 gcgggcgcct catttttctt cggcataatg aatgtctgac tctcaatatt tttcgccccg
     4501 tcatggtaac ggactcaggg caaatagcaa ataacgcgca atggtaaggt gatgtgcgca
     4561 gcaaagcgat gttagtggta tacttccgcg cctggatgca gccgcaggtg tgggctgctg
     4621 tatttttccc tatacaagtc gcttaaggct tgccaacgaa ccattgccgc catgaagttt
     4681 atcattaaat tgttcccgga aatcaccatc aaaagccaat ctgtgcgctt gcgctttata
     4741 aaaatcctta ccgggaacat tcgtaacgtt ttaaagcact atgatgagac gctcgctgtc
     4801 gtccgccact gggataacat cgaagttcgc gcgaaagatg aaaaccagcg tctgactatt
     4861 cgcgacgccc tgacccgtat tcctggtatc caccatattc tcgaagtcga agacgtgccg
     4921 tttaccgaca tgcacgatat tttcgagaaa gcgttggttc agtatcgcga tcagctggaa
     4981 ggcaaaacct tctgcgtacg cgtgaagcgc cgtggcaaac atgattttag ctcgattgat
     5041 gtggaacgtt acgtcggcgg cggtttaaat cagcatattg aatccgcgcg cgtgaagctg
     5101 accaatccgg atgtgactgt ccatctggaa gtggaagacg atcgtctcct gctgattaaa
     5161 ggccgctacg aaggtattgg cggtttcccg atcggcaccc aggaagatgt gctgtcgctc
     5221 atttccggtg gtttcgactc cggtgtttcc agttatatgt tgatgcgtcg cggctgccgc
     5281 gtgcattact gcttctttaa cctcggcggc gcagcgcatg aaattggcgt gcgtcaggtg
     5341 gcacattatc tgtggaaccg ctttggcagc tcccaccgcg tgcgttttgt cgctattaat
     5401 ttcgagccgg tcgtcgggga aattctcgag aaaatcgacg acggtcagat gggcgttatc
     5461 ctcaaacgta tgatggtgcg tgccgcgtct aaagtggctg aacgttacgg cgtacaggcg
     5521 ctggtcaccg gcgaagcgct cggccaggtg tccagtcaga cgctgaccaa cctgcgcctg
     5581 attgataacg tctctgatac gctgatcctg cgtccgctga tctcttacga caaagagcac
     5641 atcatcaacc tggctcgcca gattggcacc gaagactttg ctcgcacgat gccggaatat
     5701 tgcggtgtga tctccaaaag cccgacggtg aaagcggtta aatcgaagat tgaagcggaa
     5761 gaagagaagt tcgacttcag cattctcgat aaagtggttg aggaagcgaa taacgttgat
     5821 atccgcgaaa tcgcccagca gaccgagcag gaagtggtgg aagtggaaac tgtcaatggc
     5881 ttcggcccga atgacgtcat cctcgatatc cgttctatcg atgaacaaga agataagcca
     5941 ctgaaagtcg aaggggttga tgtggtttct ctgccgttct ataaactgag caccaaattt
     6001 ggcgatctcg accagagtaa aacctggctg ttgtggtgtg agcgcggggt gatgagccgt
     6061 ctgcaggcgc tctatctgcg cgagcagggc tttaacaatg tgaaggtata tcgcccgtaa
     6121 tttgtggttg taacgtcgca tctggtcaga tgcgacgttt gccgcatccg acactactcg
     6181 taataattat aaatccctgc cgccatcacc agttgtgatg ccacttcatg ggctttctcg
     6241 cgcccaacca gcaggtcgat aattttcaga ccaaagtcga tagctgtacc cggcccctgg
     6301 ctggtcagca attttacccg tgcatcccag acgacgcgct tgtcctgcca ttgttcagcg
     6361 ggaattttgt ctttcagcgt cgggaagccg gtcatattgc caatcggaaa gatatcgtgc
     6421 ggcaccagca cggtggctgg cgcggcgcaa atagccgcga cgatacgccc ggaacggtgg
     6481 aactgtttaa cggtttcaac cagcagggta ctatcgcgaa aacactctgc gcctttaatg
     6541 ccaccaggca gtacgatcac gtcatattcg ccatcagcca cttcgaccag cggcgcatcc
     6601 gccagcagct tcacgccgcg cgagcaggta attgccaggt tgccatcgct ggctacgctg
     6661 gcagtggtga ctttgatacc gccgcgaacc aagaggtcga tcgtggtgac ggcttcagtc
     6721 tcttcactac caggggcgag gcaaaccagt gccgatgcgc tcatattcac tctcctttct
     6781 ttttaccatt tcaaacaggc gggtgttttc cggtacggca atcccatgcg cgcgggcgcg
     6841 gcgtaagaga aaaccattga tatagtcgat ttcggtgtgg cgcagcgcac ggatatcctg
     6901 caacatcgac gagatatttt ccgctgtggc atcaatcacc tgcatcacgt aatcacgcaa
     6961 atcttccgct gaagtatgat gcccttcgcg ttcgatcact gccgcgactt cttcgcatat
     7021 ttgcataatt tcttgcggat gatgacgtaa ttcaccgttc gggcaattcc agatggcagt
     7081 cagtggatta atcacacagt tgactgccag cttgcgccac agctcggcgc gaatattgtt
     7141 atgccaggca acgtcaggca acacggtttg caaaatatcc gccagataac tgtaatcgcc
     7201 gtcctgttgc cgtgctggac caatatgcgt gataccgttt gccacatgaa taatgacatt
     7261 gccgtcgcgg cgggcggcat gggtggtggt gcccatcagt aatggctgct gaatgttttg
     7321 caattcttcg atggtgccca tgccgttgtg aattaacagt attggcgtag ttacaggcag
     7381 tgtggacgcg aggcttttga cggcatcgga aacctgccat gctttcagcg tcaccaggag
     7441 cagatcgctg gtggcaagaa aatcgggatc gttggcggtc agcgattcgt taaatatcga
     7501 accatcagtc tcaaccagat tcacactaca ataaggttgc ggtacgcgca gccagccctg
     7561 aacttcgtga ccctgtttac aaagtgctgt aagccataat tgccctaagg caccgcatcc
     7621 caatacggta attttcattg ttcctcctca cccgcaacca ctccgggtgt tcaataaggc
     7681 tatcccttaa ttgtgcatgc tgttgcgact atgcacaatt aagggatacg tcctggtgca
     7741 ggactgttgg ttatttaact ttgcaggtat tatgcttcgc atcaaaaatg aagggagagg
     7801 aaaagatgcc atctttcgat attgtctctg aagttgatct tcaggaagca cgtaacgcgg
     7861 tcgataacgc gagccgcgaa gtggagtccc gttttgactt ccgtaacgtt gaagcctcat
     7921 ttgagctgaa cgacgccagc aaaaccatca aagtgttgag cgagtccgac ttccaggtca
     7981 atcagttgct ggatattctg cgtgccaagc tgctgaagcg cggcattgaa ggcagttcac
     8041 tggatgtacc ggaaaatatc gttcatagcg gtaaaacctg gtttgtggaa gcgaaactga
     8101 aacagggtat tgagagcgcc actcagaaaa aaatcgtcaa gatgatcaaa gacagcaaac
     8161 tgaaagtgca ggcgcaaatt cagggcgatg agatccgcgt gactggcaaa tctcgtgatg
     8221 atttgcaggc tgtcatggcg atggtgcgcg gtggcgatct gggtcagccg ttccagttca
     8281 aaaacttccg cgattaatcg cgacaacctg cgctttgttc atgtcagatg cggcatgaac
     8341 gcctggttcg gcctacaaag gtttgtaaat caggaactta gtaggcctga taagcgtagc
     8401 gcagcaggca atttcgcctc gcttttatgc ctggcgaatt gcctgttcaa cctcaaagcg
     8461 attcgtcact ttgctgtcga ttttcacata agctgaatgt tcttcttctg caatcaacac
     8521 ttctttgatg ccttcagttt ctagcaaacg cacttttaac gcctcgtttg cggcaatgtt
     8581 cgccgggatt tcaatgcgca aactgctgac atacggcggt tctttcatgg tactggcgac
     8641 tgccagccac actgcggcca gcattgcgcc agcgagaaat accccctgac cgtcaaacat
     8701 gccgtcaatc cagccgccca gcgaaccgcc aatcgccacg ccaagaaact ggctggtgga
     8761 gtaaacaccc atcgccgtac ctttgtaacc tgctggcgac tctttactga taagtgaggg
     8821 caggagggct tccatcaaat taaacgctac aaagaaaagc tgcacgccga ccaccagttg
     8881 ccagaactgc gtttgcgcgt tccacaacac aatttccgca accacgatca acccgacgca
     8941 gaagacaaag acttgcttca ttttgcgctt aacttcagcg tagataatga aaggcacgac
     9001 cgagccaaag gcgattagca ttgtcgccag atagaccttc cagtgttcag ccgccgggaa
     9061 ccccgcatca gccagttgtc cgggcagggc aacaaacgtc gacatcagca aaatatgcag
     9121 acacataatg ccaaagttga gtttcagcag ccgcggttcc gccagcactt tactgaaact
     9181 gcctttcacc attccggact cacgattaag tacgtgagtg ctactgttgg gcacaaccca
     9241 aatggtcaac gcaatgccgg tcgttgccag aatagcgatc atccagaaca gcgcgtgcag
     9301 cccaagtttg tgagtgatga tcgggccaag caccatcgca atggcaaagg taatgccaaa
     9361 gctcacgccg ataaacgcca ttgctttggt gcggttttgt tcgcgcgtga gatcggaaag
     9421 cagcgccata acggcagcgg caatcgcacc ggagccttgt agcgcccgac ccagaataat
     9481 tccccagatg gagtcagaga gcgcagcgat aacgctaccg gcggcaaaca ctgccagccc
     9541 accgacaatt aatggtttgc gaccaatgcg gtcggaaagc aggccaaacg gaatctgaaa
     9601 aacggcctga gtcagaccat aaataccaat ggcaataccg attaatgctt cgctggcacc
     9661 ttgcagagcc atgccgtacg tggtcagaac cggcagaacc atgaacatgc ccagcatgcg
     9721 caacgagaat acggtcccta aaccccaggt cgcgcgccgc tcacctggcg tcattttata
     9781 atcgttcatt accacctctg ttttaaattc gcgactagtg taaagcggca aacgaatggg
     9841 gtaaacatgc ggatttttta gtaaatattt cgagagggat atgtttctaa tgctaagaaa
     9901 aaaggtgccg tagcaccttt ttaatagaga ggttttgtta ccacacagca gccagcagcg
     9961 tatgcgagtc cggtaccata aaatcaacgg acatcatcac cgagagggca gtgatggcga
    10021 tgatagagaa gccgaacagc ttgcgcgccc agattctgtc atcagcaact ttataaccgc
    10081 gcagagccat acctaaccac cagacgctaa ccgccgcggc gaccaccaga tatttatacc
    10141 cagcgtaacc gccaagagag agcatcagcg tggcaacggc aaaggcgatg atatacagcg
    10201 tgatgtgatt cttcgccacc gaaatgcctt ttaccactgg caataccgga atgtttgccg
    10261 cctggtaatc cttaaagcgg aaaatggcga tggcatagga gtgaggcatc tgccacaggc
    10321 tgaagatagc cagcaggatc gctgcgccgc tatcgaactc gccggttact gcacagtagc
    10381 caatcactgg cggcgcagcg ccggagagcg aaccaatcaa tgtgccgtag actgagtggc
    10441 gtttcatgta caggctataa acgccgacat aaaccacaaa gcccatcacc cccagccagc
    10501 aggccagcgg attcgcgcca aaccacagca gcataaagcc agcaataccc agcaaggtgg
    10561 cgtacaccag cgagacagca ggagagatca ggcctttaac cagcacccga ttcttcgtcc
    10621 tttccatctt tctgtcgata tccctgtcga tgtagttgtt aaacacacaa cccgacgcca
    10681 caaccagtga caccccaacc agcgtgtaga taaacagggg ataatcaatg ctgccctttg
    10741 aggccagcag gaatccccca atcaccgaga tcaggttgcc aaagatgatg cctggtttcg
    10801 ttacttgcag gtattgctta aacatcataa ccgccgctct tagtgcatca tcatgttgta
    10861 gttgaggttc cacataatcc agatggagcc tacaaccagg atagcgatga ttagcacggt
    10921 gaagacaaac gccgtcatgt tccagccttc atctgatttg gtattcatgt gcaggaagca
    10981 caccagatgc accagaacct gtaccactgc cattgccagg attgttccca gaattacggc
    11041 cggagaggca gcccctgtca tcaccatcca gaacggaatc accgtcagaa tgatcgacag
    11101 gataaagcct gtcatgtagg tttttacgct gccatgggac gcgccgctgt gatcggtaga
    11161 atgactcatt acatcgcccc catcagataa acaacagtga acacacagat ccaaaccaca
    11221 tccaggaagt gccagaacag gctcaggcac atgatgcggg tacggttagt gctggtcagg
    11281 ccgcgacgag cgatttgtac catcagcacc gccatccaga taagaccgga agtgacgtgc
    11341 agaccgtgcg tgccgaccag cgcaaagaac gctgacagga agccgctgcg atccgggccc
    11401 atgccgttaa caatcaggtg atggaattca tagatttcca tcccgataaa tccggcgcca
    11461 aacaaccagg tcaacgccag ccaggagata acctggcttt tgttgttttt gtacatggcg
    11521 atagccgcca tgccgtaggt gatggagctg aacaacagca agaaagtttc aaccagaacg
    11581 aacggcagtt cgaaaatgtc cttacctgtc gggccgcctg cggtgccgtt caccagaacg
    11641 gcataggtag caaacaagat agagaacaga atgcagtcgc tcatcaggta gatccaaaat
    11701 ccgaagattt tggttccgcc tgcatcgtgg tgcccgtgtt cgtgcgcgtg ggcagtcgcg
    11761 tgcgtcaaag tatcagttgc catttttcag ccctgcctta gtaatctcat cgaaatgctg
    11821 gttttccagt ttttcgattt ctgccaccgg cacgtagtaa tccacgtcct cgtcgaagct
    11881 tttcacgatc caggtgatga tcatgcctgc gaagccaaca atcgccagcc accagatatg
    11941 ccagatcatg gcgaaaccga agatggtgga gaaagctgca atgacgatac ctgcaccgct
    12001 gtttttcggc atatgaattt cttcatagtg gtcaggcttt ttatacgctt cgcctttctc
    12061 tttcatttcc cagaatgcat cacgctcgtg aacgtgcggc acaacggcaa agttatagaa
    12121 cggaggcggg gaagaggttg cccactccag cgtacggcca ccccacgggt cgccagtcag
    12181 gtcacggttc tggtcgcggt cacgaataga aacgtacatc tgaataacga ggcagagaat
    12241 acctagcgca atcagtactg caccgctggc tgcaatcatc agcatggtgt ggaactgcgg
    12301 gtcaatctgc tggctcaaac gacgggtcat gcccatgaag cccagcgcgt acagtggcat
    12361 aaaggcaacg aagaagccga tgatccagaa ccagaacgcg cgtttacccc aggtttcgtt
    12421 cagtttgaaa ccgaacgctt taggccacca gtaggtcatc cctgcgaagc agccgaagac
    12481 cacgccgccg atgatcacgt tatggaagtg cgcaatcagg aacaggctgt tatgtagaac
    12541 gaagtccgcg cccggtactg ccagcagcac gccagtcata ccgcccaccg agaaggtgac
    12601 gataaaaccg atagtccaca gcatcgcaga atggaacacg atgcggccct gatacatggt
    12661 gaacagccag ttgaagatct tcaccccggt cgggatggcg ataatcattg tggtgatacc
    12721 aaagaaggcg tttacgttcg cgcccgcacc catcgtaaag aagtggtgca gccaaacgat
    12781 gaacgacagc acggtgatac agacggttgc ccataccagc gaggtataac caaacagacg
    12841 tttacgcgag aaggttgccg caatttcgga gaacacaccg aaaacaggca ggatcaggat
    12901 ataaacttcc gggtggcccc aggcccaaat caggttgatg tacatcatca tgttgccacc
    12961 catatcgttg gtaaagaaat gggtgcccag atagcgatcc agggtcaaca acgcgacggt
    13021 aaccgtcaga attgggaagg aagcaataat cagtacgttc gcgcacagtg atgcccaggt
    13081 aaatactggc atcttgaaca tggtcatgcc cggtgcgcgc atcttcagaa tggtaacgaa
    13141 gaagttgata ccggtaagcg tcgtacctat accggatagc tggagactcc atatccagta
    13201 atcgacaccg actcccggac tgtactctat tcccgatagc ggtggatagg ccagccagcc
    13261 agtctgcgca aattcgccca cgccgagaga aacgttaacc agaatcacac caacaacggt
    13321 aaaccagaag cttaagttgt tgaggaacgg gaacgcaacg tcacgcgcgc caatctgcag
    13381 cggaaccacc aggttcatca gaccgataac gaaaggcatc gccacgaaga agatcataat
    13441 cacgccgtgc gcggtaaaga tctgatcgta gtggtgaggt ggcaggaagc ccgcttcgcc
    13501 cgccgaggca agagcctgct ggctacgcat cataatggcg tcagcaaaac cacgcagcaa
    13561 catcacaatc gccacgatga tatacatgat accgaggcgt ttatggtcga cggaggtcag
    13621 ccactctttc cacaggtagg tccacttacc gaagtaagtg atcaggccaa cgagcgccag
    13681 acctcccaaa ataatgccag cgatcgtaac catgacgata ggttcatgga acgggactgc
    13741 atcaagtgat aattttccga acatctttat tcttcctcaa cccctttaat gggcggattc
    13801 cgcgtggctc atgtccatgc cttccatacc ttcgtgtgcg ctgtgctcac cttctggctg
    13861 ggtcatgtcc atgctcttac cgtgagccat aaacttgtta atcacatcag caaacaagtc
    13921 tggtttcacg ttggagaaat attccacctg gttgtattcg ctaggcgcgg ccagtttttc
    13981 gaacgcagcc atgtcagaca tggtgttcgg cgactgcttc gcttttgcga cccactggtc
    14041 gaattcggcg cgatccggtg ttgcaatagc tttgaacttc atgcctgaga agcccgggcc
    14101 gctgtagctg gcggagatac cgtcataagt accgggttcg ttggcgatca gatgcaggcg
    14161 agtctgcata ccggccatgg cataaatctg gctacccaga cgcggaatga agaaggagtt
    14221 catcacggag ttggaggtca ctttgaagta caccggagtg ttcgccggga aagcgatttc
    14281 attcacggta gcaatgccct gttccgggta gatgaagaac catttccagt ccatggaaac
    14341 cacttcgatg gtaatgggct tctcgtcgtg tgccagcggc ttgctaggct caagagcgtg
    14401 agtggttttc caggtcagta ccgcaaggaa gatgatgatt aagataggta ccgtccagac
    14461 cacagcttcc actttattgg agtgtgacca gttcgggctg tacttagcat ctttattgct
    14521 cgcacggtac ttccaggcga aaccaacagc catcaagatt gcgggaataa cgacaatcaa
    14581 catcaggcca aatgccgtca gtatcagtga acgttgctcc agaccaatct gtcctttggg
    14641 atctaacagc gcagaattac agccactgag caatacagtg cctgcaaata atgacaacca
    14701 tcccaaactt ttattgtatt tcctgagtct catttaacga cctcaattcc acgggatctg
    14761 gtggcgttta aagtgtgtgg gcattttacg ggaaggttac attactgtaa acatgattaa
    14821 attcctgtta cttggatttg gctgtcaggt cacatatgtt gcaaaacata tcaggttgat
    14881 tttaagaagc tgttgcaaaa gggcgttata acaaaagggg agtgcagaag gagtctgggc
    14941 gg
//
LOCUS       DAZUGK010000108        14757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZUGK010000108 DAZUGK010000000
VERSION     DAZUGK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.109"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..294
                     /locus_tag="ACHY5W_002900"
     CDS             <1..294
                     /locus_tag="ACHY5W_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFS4447772.1"
                     /translation="PQYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEH
                     SDPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(515..3235)
                     /locus_tag="ACHY5W_002901"
     CDS             complement(515..3235)
                     /locus_tag="ACHY5W_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4447773.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(3247..3579)
                     /gene="mobA"
                     /locus_tag="ACHY5W_002902"
     CDS             complement(3247..3579)
                     /gene="mobA"
                     /locus_tag="ACHY5W_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFS4447774.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            3811..4146
                     /locus_tag="ACHY5W_002903"
     CDS             3811..4146
                     /locus_tag="ACHY5W_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFS4447775.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4232..5080)
                     /locus_tag="ACHY5W_002904"
     CDS             complement(4232..5080)
                     /locus_tag="ACHY5W_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4447776.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            5430..5625
                     /locus_tag="ACHY5W_002905"
                     /pseudo
     CDS             5430..5625
                     /locus_tag="ACHY5W_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5659..5910
                     /locus_tag="ACHY5W_002906"
     CDS             5659..5910
                     /locus_tag="ACHY5W_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4447777.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(5941..6186)
                     /locus_tag="ACHY5W_002907"
     CDS             complement(5941..6186)
                     /locus_tag="ACHY5W_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447778.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(6186..7076)
                     /locus_tag="ACHY5W_002908"
     CDS             complement(6186..7076)
                     /locus_tag="ACHY5W_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS4447779.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            complement(7141..7407)
                     /locus_tag="ACHY5W_002909"
     CDS             complement(7141..7407)
                     /locus_tag="ACHY5W_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447780.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            complement(7500..7934)
                     /locus_tag="ACHY5W_002910"
     CDS             complement(7500..7934)
                     /locus_tag="ACHY5W_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFS4447781.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(8662..9162)
                     /locus_tag="ACHY5W_002911"
     CDS             complement(8662..9162)
                     /locus_tag="ACHY5W_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4447782.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(9624..10221)
                     /locus_tag="ACHY5W_002912"
                     /pseudo
     CDS             complement(9624..10221)
                     /locus_tag="ACHY5W_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10218..10937)
                     /locus_tag="ACHY5W_002913"
     CDS             complement(10218..10937)
                     /locus_tag="ACHY5W_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFS4447783.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(10934..11182)
                     /gene="psiB"
                     /locus_tag="ACHY5W_002914"
                     /pseudo
     CDS             complement(10934..11182)
                     /gene="psiB"
                     /locus_tag="ACHY5W_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            complement(11177..11416)
                     /locus_tag="ACHY5W_002915"
                     /pseudo
     CDS             complement(11177..11416)
                     /locus_tag="ACHY5W_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11461..11947)
                     /locus_tag="ACHY5W_002916"
                     /pseudo
     CDS             complement(11461..11947)
                     /locus_tag="ACHY5W_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(11907..12125)
                     /locus_tag="ACHY5W_002917"
     CDS             complement(11907..12125)
                     /locus_tag="ACHY5W_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447784.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            complement(12125..12808)
                     /locus_tag="ACHY5W_002918"
     CDS             complement(12125..12808)
                     /locus_tag="ACHY5W_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFS4447785.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            complement(12884..13195)
                     /locus_tag="ACHY5W_002919"
     CDS             complement(12884..13195)
                     /locus_tag="ACHY5W_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447786.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(13192..14094)
                     /locus_tag="ACHY5W_002920"
     CDS             complement(13192..14094)
                     /locus_tag="ACHY5W_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFS4447787.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLTGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            14367..>14757
                     /gene="parM"
                     /locus_tag="ACHY5W_002921"
     CDS             14367..>14757
                     /gene="parM"
                     /locus_tag="ACHY5W_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFS4447788.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLRE"
BASE COUNT         3523 a         4175 c         3717 g         3342 t
ORIGIN      
        1 ccgcaatatg atacagatat cgaatcccgg gcaaacgaac tgtggaaaaa ggctgtgcac
       61 actatcaata acacaaccag ggaagaacgc cgggtatgtt atatcacact gaatcgtccg
      121 gaagaggaac actctgatcc tgaagatata ccttcagtga aggcgattct gaataccctg
      181 ctccccctgg agatactgtt accggtcgct gaaaataata aatcgcagac aataaataat
      241 gatcatcccc agaaatcatg tggagcacaa ccaacaactg acgaaaaata ctgaaggaat
      301 acaaatattt tctggtgacc atatctgtca atcagactgt aaaaccctgt tcactaagtc
      361 cggggtttta cggcgcactg gataagggcc gcagacacgg cccttaattc agagttaacc
      421 gggtataaac tcaattcagt gactactccc gcccttattg acgaaggttc ctgccgtttt
      481 tgggctgaat ctttgcacca tcacttatcc gggcttacat tccaggtgat ggtggctccc
      541 atgttttctc ctgccgtaac gtataattat catcatggct caccgataaa cgggtctgct
      601 gtctgatata ttcccggtaa tactgctcac tgcgttgacg gtgcaaatcc acgtccggac
      661 gagtaatggt gaagtgtccg tttccggtta ttcccgctgc attgtgccag gccaccattt
      721 ctgcaaacac atcattaaac tgctggtatg aaccttccgg ctcaaaacca agctttccgt
      781 tgcggctgaa cagaactggc gcaattaaat caagcttctc tgccagttca tttcggtccg
      841 tgtgatggta gaacacaacc cgatctccgt aatccgtaca gacatttttt cctgtccgcg
      901 tatccctgaa atgaacactt ccatttttct gaagacgagc ttcaagtttt gcaacattat
      961 taaaaagtgg cgttcctccg ggttcgcaga gtacgacaca acgatcaaca ttagttgtgc
     1021 gtcttttatc cttattacgc gagcgacgat cacgataatc ccagccacgt aactgcgata
     1081 ctgcagccct gtctccctga gccgcctgtt gttccaccca ctgccggtag gacaaaggat
     1141 accacttacc ctccgcaatc agtgacaacc gctcttcctt cacactctcc ttcagcctga
     1201 tcagcgcctg catacgctgc acttccgcaa tatggtaatg cagcttacgc aattgtggat
     1261 cccggaactg cacacggata tacgccttgc gccgacggca ggcagcatga atttcccgta
     1321 accgttctcc gtaacgtaag tcgggtttac gccagtgctc cttccatgca agataacgcg
     1381 cacgcaaatc ttcccgcgca gctgcacggg cttcacggcg ctcacgacga atctcaggat
     1441 cgcgccggaa gcctggttcc acttcatcac tggcagcaag ttccggattg taacgacatt
     1501 caggcttcac ccgttcgaaa atatctgcag ccgcaatttc aaatggcccg gcctgaggct
     1561 cggcacggga taacgtcaaa tcaggatgga tactgctggc cttcaccggt gtcagctcat
     1621 gattaaacgc atccacaatc acaagaccat gatgctgttt ctgcagcata agccctttac
     1681 gcgcaaacat ctcatgacat gcctgccagc tgaaatcccc gtcagctatc atctcttcaa
     1741 tcaggctcct gcactgaata aggccgtatc cccacagact ttccttatca gaaaacagtt
     1801 cgcgctttat tgctccctgt ggacctgtcc ccatccgcat acgataccat tcgtaatcac
     1861 tgacagcctg agtaccaaga ttacgtcccc ggaaccgttc tccgggtata acctgagtaa
     1921 aaatatcctt cggtacaggc tgccatccac catccagatc ctgcatataa tccagcgtca
     1981 ttaacggcca cacactgtct gcacgtactg gtgtctgttg tttatcaaat ccatcatgca
     2041 gtaccagatg cccgtgctgg atccccatcc ataatccagc cgttgccaga gttctgtgca
     2101 catccaggca actttccagc tcaccatttc tcgccatttc cggtaagcgt tcggcaaaat
     2161 gacggcaggc gtactgtttc agactctccg tttctgccac tttttccggc cggatatcaa
     2221 cgtttatggc ttccggatca taacgaccgg gtgtccccac ctgactgaaa atatctgtcg
     2281 gtacaggctg atattccccc agtttccgcc ccagtttttc agctgtccat aacggtccga
     2341 acgaactgag tgcaacacct tcccgcgctc tgtcccagcc atccttcacc accagttccc
     2401 cttcctgcat cgtgatatac agaccatcac tggccagccg tttatgcaaa gataaccagt
     2461 cctgtgcggg ctcttcacgc agcccggcaa tcgacatctg aacaatatat tccctgaacg
     2521 tctgcttttt cccgcgtcgc caggcattac ggcgctcacg taccagcgct gttttgcgca
     2581 caatacgatt gcccggtgca tgcacaaagc agccattatc cggcgcgaat ccgtgcttaa
     2641 tttcaagttc acggcatgca cgactgagtt tttcctggct ccacggaagg caattgatat
     2701 agccggtttc agggtggaca cgattcactg caacatgtac atgcaggtta tccgtgtctg
     2761 tatgaacagc tgccacatac tgatgttttg acagctcaag ggatttcagg gtgtgacgca
     2821 cacagtcaaa aagctgctca gaacgcggac tttcgtgcgc aggccaggag agtatatagt
     2881 gaaaaacagg atctgtatca tctttggaaa aatgagcaag gtctgctgct ttctgcattt
     2941 cttcagccgc agattcaaga gacaaacagt tatgaaagca ggtcacacca tagaaattca
     3001 cccactgact gccgtctttc atcacatcaa tcagggaaac aaatttttca ttacgcagac
     3061 gtgttgcata atcaacaaga cgtctgaagc ggttccggtg cggaatctca gctttcattt
     3121 ccgtgtctgg cctgagttca tcttccggca catcatcccg cactgaggtg tacgcaatca
     3181 ggtcttcaaa agaagacttt ccgtcgcgtc tttttttcgg aatgatggca ttcatcagcg
     3241 attatctcag cgtcccatta aatctcttcg cagagtatta gcaagctctg taatagcaac
     3301 gagaacctct gcatactctt tatctcccgt gcgttttcct tccacaaaca gatgtttctg
     3361 cagacgacca agacgcatca gctctttcaa aaaatcttcg tcaatgcggg catttatctg
     3421 ttttcccagc acagtttttc ttatcagagt tgaaactgtc agtccgtttt tttctgcctt
     3481 ttctttaaca atctcagcct cttctggtga aaaacgaaca gcaatgagga cattcttttg
     3541 ccgtttctca ctgccacttc gggttttctt ctcactcatg gtctgctcct taccggggtg
     3601 tcggggctaa gccctgacca ggtggtaatc gtatagccgt gcgtgcgcgg ttatacgatt
     3661 acacatcctg tcccgttttt caggcattat agcctgttat ttttgtgcat catagtctta
     3721 tgcagacggc agaggcgggg cgcagctctg ccaggaggca gtttttgatg ataaaatcag
     3781 acttcaacat gaaacggaga aaaagtgcca atggcttcta aaaaatttta ctcagatgat
     3841 gacatccagc tggcaaaagc tgctttatca gaattgccgg acctcaccgc tcaacgaaaa
     3901 acattgcgtg attttcttga tgccattcgt gatgacatca ttgtcctggt aaggacaaag
     3961 ggttatacac tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga agtaggagag
     4021 aaggcacttc gtgacattat tcgggaagca gaatcgaaaa aaccatcccg ccgttcttcc
     4081 tctaaaacag cacctaaaaa gacagattca ggcaggaaag atggcatcga tatgaacaac
     4141 aattgatgtg acaatgtcac atcgtattct gagattatga tgattacaga aaaaaaagcc
     4201 ccggcataaa taccagggct tttctgttta atcagactcg tggcggcaat acaccaggat
     4261 aatgccgtgc aataatatca ttcagttttt caacaaggtc aggacgctta aagacaatgt
     4321 gtacggtccc tttctggtag ccacgcaccg taaaatattc cccttcaaac aactcaccga
     4381 tattgtcgcc gttcccgaga aattcaccat actgcgcccc gatgctgttc ctgaagtcag
     4441 gaacgttgcg tccctccagc agataaaacg gacgtaccag gtcatccagt gcctcccgtc
     4501 cgctgcagtc cagcgtcaca cgcccgttac tccagcggta cagcaggttt tcaataatga
     4561 ttctcttgcc cagacggcag ggattattgg ttctgtaatc ccatgacaat ttccgccaga
     4621 tatcaatcag ttcctgttca aatgtctgca tcttactggc attcagatgg cggaatgttg
     4681 ccagtacatt gtccagggtg atttcaggac aggtgtcgct catcagctgg ctgttccact
     4741 catcacgctg gcatgaactc atgaaggtgt acatgcccgt ataagtcatc aggcgatccc
     4801 agattttccg gtcggcaaga cgggtgatat tcttttttgc ttcttccggt ttatccatgc
     4861 atcgaacgga gtcagtcacg cagtcttcaa acccgtagag aaaaatgtca ccggaagcat
     4921 cgagcagaat cctgcgcgct gtcgccaggc attccatcgc ctgcatgaac agtgcaatac
     4981 cttcgttacg ctgcgcgatg atgcggtcga tagccataga cggaataata tctgtcgtgc
     5041 cggcagtatt caggtcagca gtgggtaaag tctgattcat catattgtct cctcatcgtc
     5101 acggctgccg ggtcagcctg gtcatttccc ggaggagcga agctccttct gccgtccact
     5161 tcacaggggg ttcttcccgc agtggacctt tccgatgggg gacgctcacc acgtcacggg
     5221 gcagttgtcc gggggcagac gcaaaagttt ttgacgcctg cggcaaaaag ttttgtggct
     5281 ggtacatctc tcccctggac gactgaaacg ggatgtggtc gtgtatgtac ccccggaaag
     5341 tccgtctgcg gagaacgggc cgcagtgcgg cggcagacag gcagagacag ctgcgccatc
     5401 aggcgcagtg gccttacaca ccgtcaggtg tgcagggagc gccgcatccg gcgcgactga
     5461 agaaaaacga cagcacaaca atggcatgtg gtggtcactg gcgcaccact cccgcacagg
     5521 tgaaaacaga gaaaacagca cgacacctac aactaaaaag tgaaatttct tcactttttc
     5581 actcttattt atcgcgctga cctggtgatt atgatgataa aataaagtga agttttttca
     5641 ctttttcaaa ggtgttttat gaccggctgg gaactgcgaa tctggcgaaa aagcatgctc
     5701 tggtcacgcg aaaaagcggc cagagaattt ggcgtgacac agagaacatg gcacgcctgg
     5761 gaaaatgcgg aacaggtgga tgtcaccgtc tggaggacca cccaggcact ttcagtccgt
     5821 gatctgctgc ctcacatgca ggggatgcgc aaggcagata tcatcaggcg tctggaaaat
     5881 gaactggggg aaacggcagg gaatgtgtga gcgtgattca cagcctggca gaatgccagg
     5941 ctactgctca tcaggaaaca gctttttcag ttcttcaccg gaaagtccgg ttgcctcctg
     6001 aatccgttcc ggcgtccagc ccatatcccg cagccggcag gctgcttccc gcgccacttc
     6061 aagcgcacct ttcttaaaac cttcgtcgaa acctttctgt ataagacgtt ctgcaatcgt
     6121 catgctgttt gctctgctcc tgctgacaaa aaaacccggc attttgccgg gctttgtggt
     6181 tctcatcagt gagtgatgtt cttcagttca gcttcggaaa gtccggtagc ctgcttaatc
     6241 tgctccggcg tcatccccat tttctgaagc tggcacgcaa tagccagggc tttttcaagc
     6301 gctccttcct ggcgaccttt ttgctcaaga cgttcagcga tagtcatcag tttatcctca
     6361 tgctgcggca gacgctcagc cagcgcccgc ataaattccg gtgatacaga gtcccctgcg
     6421 ttaacgatgt aatgaatcag aacacgcacc tgctcgtctg aaatttccac catgacctgc
     6481 gggagtttgt ccagcagctc catcatgtca cgatggcgga tatgcttcat cagcagcgtc
     6541 agtgcggcca tccggcgatg gttcatgatt tcattgtcat caacgacagt gatatcaacc
     6601 agcggaaacg gctttgtgta tattttagcc gcaagcgccg gttcctcaaa acagtccagc
     6661 cagttcatgc tgtacgggta cgggctgcgc tcaccatgat agaacagcac cggaatcacc
     6721 agtggcaatg ttttgtgctg ctccagatgt ctctgcatcg ccgccacact gtaacgtaac
     6781 atgcggaaag gcatgtacag attttcggtg ctctggtgct cgatgagcgt ataaacatag
     6841 ccgtcttcac ccccggtggt tttcacactc cagaggatat cgctggcgta ctgatgcagg
     6901 tccggctcaa caaaggtggc gggttccagc ttcagcgtgg acaggtcgca caactgccgg
     6961 tactccgccg gaagatgcag ttccagaaaa tctctggcga cgtcgggatt cgccaggaac
     7021 gaccagaacg cggcatcatg cggcgtgggc gttgtggtgt ttttcttctt actcacggtt
     7081 cctcctggtg agatgtctga acgtatcctg ccgcctgcac aggcagacgg caagggttaa
     7141 tcagtggcag gcccgctcaa tttcaagcgc gcggagcagg tggccccatt cgctgacttt
     7201 catggcgggg atacgtaccc gcttctgctc actgcagcgt ctggcgatat gaccgctcat
     7261 cgccaggacg tccgggttaa tctcatcctg tgccagctcc tgacgtctga cgcgggtatg
     7321 gcggtgcagg ggtttattct cccgcaggca ctcttcacgc agacgcttta catactcggc
     7381 ggttgcgaag ataatggatg gtctcatggc ataatctcca tagatagtga ttgagatgcc
     7441 ggacttccgt acggggaagc gccggcggag aaaggcaggg tttatcgccc tgcctttttt
     7501 catttcggac agcggcgcag tgccagcgcg agcgctttca tcttccagcc ttccggcgtg
     7561 cggtacatca ccttccagta gccgtcgatg aacacataat ggaagcgcac ctcttcagtg
     7621 ctttcagtgg caatagcctc catgaaagaa cggtactcac gcgcctcact gtccatccgg
     7681 aaaccactgt cacgtccgta ataagccgtc acaccgtcca ccgggttctg aagcgtatgc
     7741 ccggcggatt tgtcgcaacg gggaccgagg caacggatat ctccgtgttc aaccagggct
     7801 tcggccagct gaccggtggc atagtgggta tgcaggatgc gaccgttcca ggtcggctcc
     7861 ccgtgcaggt ggcagtaaac ggtacggaac accccgtcgc ggcaaaaaac ggaaatcgtg
     7921 ctgctgactg tcatgaaaac gtctccttct gctgaatcag gtcgccgggc cgaccggttg
     7981 ctcccccgga ggggcgaaag ccccttctgc cgtccacttc ttcggggttt ctccccgcag
     8041 tggacctttc cgatggggca gcgactgcca cggcacgggg cagttgtccg ggggcagacg
     8101 caaaagtttt tgacgcctgc ggcaaaaagt tttgtggctg gtacatcttc ccctggacgg
     8161 ctgccccgtg ttgtggccgc ttcagccccc ggaaagtcca cagcgggaaa caggccgcag
     8221 gggtggcggc agaagggttg actgcgccat caggcgcagt ggccttacac accgtcaggt
     8281 gtgcagggag cgccgtttcc ggcgcgacag gggcggcgcg accggaacgg tcgctgcagg
     8341 cctggttttt cagacatcgg accgtagcgg cagcccgaaa ccgcgccagc gggttcggcg
     8401 gagactggcg ggtgcgccag cagctgcctg tcggagtcgg gccgacgacc ggatgccgcc
     8461 aggcagccgg gagccgaagg gtgtccccgc ctccgggcat cagaacaaca gaaacgacaa
     8521 aacagaaaaa tgcctcacac catgacgggc aggaagggca gcgccgcagg cgcggccctc
     8581 ctgtatttca ggcaggcgta agcctgcctg ggttttccgg cggcccggca ggccataccc
     8641 ccgccggagc gggggagtaa atcaccgacg gaacacatga ccgtcaataa aggtgaagga
     8701 gtccaggaat aaatcccgcg catacgcctc atagtcaaaa tagagcgcca ccgtctccgg
     8761 cacgtcagcc agcaggccgg tatcactgac gaactcaacc gcaaaagcct cctcactgtc
     8821 agcctcgccc caccaggcat cgcggaaggt gtcaaaatcc gtctcaccgg tatcatccac
     8881 ccagagacga taagcctctt cgcagccttc atcccgtgcc tggcggaagc cttcaaccca
     8941 ggcccagttg atatggcatt cggaggccat attcaccggg aacccctcat aatcctgaaa
     9001 catcagttca ggatcggctt catcctggtg aagagcacgg caggcggcga aaaagtcgcg
     9061 ttcatcatca aacgtggtca ggtcaaacca gcggccagcg atgcttccac agttgtattt
     9121 atgccaggtt ccaacgtata cagcaggtgc aacaacagac attcccgaat cctccatagt
     9181 cacatgttct gcgacaccgg gcggtgccgt gcctccatct ttgccgggcc tgcagggatg
     9241 caagggcgca agggaagccg cagcccgacg cgcacggctg acgggagttg gagggtgaga
     9301 ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg
     9361 gtgaccgcag cggttcatct taccttgcat cccgcccgca gaagcccggc tacgatggag
     9421 gcacggcaca gcccgcacac gacgctgagg ccacataact gaaaacccac aaggccatgc
     9481 cccgcatggc cgggtaaaag cggatccgcc ggcgacttcc ttattccggc gagtgcagag
     9541 gacacgccgc atgacctcca gaccacccct gatatgaaac gatcatgatc ggcgtgtccc
     9601 tgcgaaaaat atacatgcct ggttcaccgg agaatatctg tgatatccac aagttttctg
     9661 tcgcacagcc agtcctgcgc cgcacgcacc acgctactgt ccggtttcac cgctttcaga
     9721 gaagatgcca gtcgtttgat cagcatcacc agttcccgtg tctgtatgtc cggcattgtc
     9781 tctggaagtg cagagggact ctcatttccg acgctcgtac catgcgcttc cagtgtggca
     9841 cggacaaacc aggcggcatc caccagttgc tggcgaatcc aggccagctc ggcatatctg
     9901 tcacgggctg ccattcgctc acccgacagc gccttgctga tatggctgcg caccagatta
     9961 tccaggagtt cctgcgcttc ttccggtgaa aagccacctg gtatctctgt gcgcaacagt
    10021 cctttcctgt acagtagctg cagcagatcc aatctgcgac cgcactgcaa ctatcagtac
    10081 ataacggaca atattcgcac tggcggtggt ttctgtatca ttcagaagtt cctccggatc
    10141 attaccaccg gtattgcaga gcaacggcaa cagaggtcgc atccacacgg gaacctgata
    10201 ttgttcgtct gatttcatca tattgcgttc tccagcttgt tcgggatcat ccactgctca
    10261 agtgcacgta cctgtaccgg tgcaccacga ccggcctcac cggcttcaaa aatcagccgc
    10321 cagagtggtt cctcctgcca gaaaaaccgg tcaaatgatg acagtgatgg aaaacgcccg
    10381 caccaccccc agaaaatcgt ttccagatca tgctcctcaa caacaccaga ccagcgggac
    10441 aaccagcttc cgaccgtggc gggagtatga aacgcaaggt caatctgcgc ctgagcaaaa
    10501 catgtctggt attccagttc ccgtcgttct gcctcacgtt cccgctggcg gctgtatttc
    10561 tgctctgtca gcgtggtttt ctgacgacga cgctcacctt cacgaaaagg aacggacggg
    10621 aacagacgat gccccgcatc ggatggcagg ggaagaacac agacttcacc aaaactttca
    10681 atcagtttat ccacggcagc cagccacagg agacggttgc catgaaattc ctccttcgtc
    10741 agcgcagggg caaaaaagcg gagatcggtc agcgaaatcc ggcgactgcc attcaggcag
    10801 cgaaagaaag cgctggcata aggccatgct gaaagtgtgc gtccctgacg acgacgttgt
    10861 tctgtgacag ccacagcctg catggcggcc tgttgttctg cgctgagggg aatcagtgcc
    10921 cgtgaacgtg cgctcatgcc tgacctcctt ctgccatcag gatatggatg atgtcgttaa
    10981 cactgtatcc ctgcgcatca agtgtcgcgg caagcgccag cgtgtgactg atagcgacag
    11041 gcgtgaagat atgttgcacc tgaacgacgg cgaaaggctg tccaccacaa ttcaccagaa
    11101 ccagcatcca gagctgagag acgtctcccg gcgaacataa tgccaggtgg aaacgttcgt
    11161 gggctggcgt gaaaccaagt tccgccccgg ttccggtcag tttctgaatg gcggaggcca
    11221 ggtgcggcgg cagtacgcca tcgtggcggt taacgagtaa cgtcgccagc cggggcgcat
    11281 ccagccgcac gtaaatgctg atccagcccg gatacttcag gttacgctgg tcaacatccg
    11341 tctcttccgg ccagccctgc gccagcagca ggtcgcgggc atgacgacaa atcagtgcat
    11401 tcagcgtttt attcatgatt catggtctcc ctgaatcgcc gcccggaggc ggcgataaca
    11461 tcagtcgttt tcgtgcgtgt tgtttttcag caggatgcgg gcctgattaa ccaccgcctg
    11521 acggcgggta acgcattccg ggacggcgag gccgtaatca cgggcggccc attcacgctt
    11581 ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc agttcagcag ggacggtcac
    11641 ctggcggttt tcgctcacgt tgctgataat ctccatcgcc gcggaggaag aaataccgga
    11701 ctcagtccgc tggtcttccg ggatgtcctc caggcgcgcc aggatggtcc tgatttcatg
    11761 atcggccagg gaaatatcca ttccgtcagc gagggcctga atctcttccg gggaccagat
    11821 aaccagcatc aacggcgtgt ctcccggata ctttgctgcc agcgcccggc agagcgtttc
    11881 gcatgttccg tacatactct ttcccctcaa tctttgaagc agtaatccag ttccagtatt
    11941 tcgttatatg ccttcataaa caggtggctg gcacgaaaga gcgtctgacg caccagacgt
    12001 tccggcaggg tgtctgcatc gctgtaccgc tgctccatct cattcatggt tgtgcgcagg
    12061 tcgcacatgg tgttggccag ttcagccacc tgttcacgca tatcttcaaa tccgctgtaa
    12121 ttcatcatgc tgcctccggt tcaaacctgt tatcatttgc ggccccctgc tgcatggccc
    12181 gttgcacggc ggcaaggcgt tgctgtccgg cacggtgata ctgttcaagc agctcgatac
    12241 caatataccg gcgtccggac tgaagggcgg ccacgcaggt tgaaccgctg cctgcaaacg
    12301 ggtccagcac aattgagttc gggtgtgtga agctctcaat cagcggttgc aggctggtaa
    12361 cgggcttttc cgtcgggtga tggcggttgc ccgaatattt ccagcccagc acatccggca
    12421 gcgggttacg gggccgggcc ggactgccct ttgccaggat gtaagcgcat tcgtggcggt
    12481 acgcgacata cgcagatttg gatgtgtagt ttttggtgaa cacgaggtgc cctgccacgc
    12541 tgaatccggc gcgtatccag accgccataa agcggtcaat acggtgccag ccgtagaagc
    12601 tgaccatcag cgcgtctttt ttgagtacgc ggtacatctc attgctggca ggttgcagcc
    12661 agtcatcatt aacgtcaccg gcaatggtgc gcccggaacg gtcacggaaa ccgacgaggt
    12721 acggcgggtc agtcagtatg aaatcaatgg cgttatcagg aatacgggcc ataacatcga
    12781 tgcagttacc gaggacaaaa cgggacatac aaaactcctt ctcttacaga gggcaccggg
    12841 agggcacccc tgtggtgccc gttacccgct gcctgtgggc tgttcaccgc gtccggtaac
    12901 gggtcaggcc gaccgtggaa taccgcagcc ttcgtctttt tgacggcaag gctatgccgc
    12961 gaaagcggct tgaagggcgt tcatcagaaa agggcgacgg gctggcgtac actgtcaccg
    13021 acgccagccg ggagcggtaa aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc
    13081 acagacagcg tttatgccgc gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac
    13141 atcctttacc ggaaaagaaa cgggcagttg cccgcccgtc tccgtcatac ttcatccgcc
    13201 ataatgcgcc accttgtcga ctatccacgc aggtccggtg acttccggca actcgtcatc
    13261 atctgtcgga tgtgaccatt ccagctcccc ccacagcaca tcaacaagct cgccacgctc
    13321 atatcgctgc cagccacaga aattgcagcc ctgttcggca taccagtgct ccagtgtgca
    13381 gctgaaacgg cggcttaatt ctgcaataac gtcgctttcc ggctgatacc acggcgtgtc
    13441 gaaatcaacc tgaatgaagt tgtctccctg actactgata ttcacctcat accccacagg
    13501 ccacttcacg ccatactgtt ccgtatacca gtgatatgca gaaggaacac cgttcagaac
    13561 accaccgtta aagccattca cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc
    13621 aaacggcatg gttttttccg gcagtacgtt gtcacacaga cggttccacc acaccgatac
    13681 atcctcgtta ctccagaagc cacaccagtc acctcttttt gcggtgaaaa gtgcggtgat
    13741 ggtatccctg acatcgtccg gtaatccttc ccagcgacgc tgtccagtac cgctctgcag
    13801 ccagagttca tgcagcatca ggcagttttg ctcatccagc agtacaccgt tctgcaggta
    13861 tgtcagccag cgggtgaacg cgatattctc cggcgataca acgccacgcc cggcagccgt
    13921 cagtccgggg catggttcaa accgcacatc ttcagtggtc tgcagaagtc cggcacttcc
    13981 ggtcagaaac agctgaatac cttcatttgt ggcgcggcga taaagcggtg tgacggcacc
    14041 gctggccagt cgtttaatct cagcaatctg tgccggttca ccagaaaaat gcatacgatt
    14101 tgagcaccag tttggcatgg gaattctcct ctcacatgcg gcctggttaa gtagtttccg
    14161 tctcagttcg aaacgcaaaa tgagaaatcg atagagactc aaactgagtc acaacaagaa
    14221 tgcaaagaga ctctaaaaga gcctcatcgc ttgacatgaa cccaaaatga gacacaataa
    14281 ggaacaacaa aaaactcaaa aagatgcatc tagataaagg atgctcaatt tgagataaat
    14341 aaaaactcaa aacgaggcaa gaaaaaatgc taaaagtatc atgcgatgat ggttcaacta
    14401 acgtaaaact ggcctggctt gaagatggcg aggtaagaac cagtctgtca ggaaacagct
    14461 ttaaagaagg ctggaacccg ggactgttta atgccggcaa agtttacaat tacgtcgtgg
    14521 atggaaaaaa atacacctat gatttgggca gtactgctgt cataggtaca acacatgtca
    14581 gttatcaata cagcaccacc aatttgcttg ccatacatca tgcactgctg acatccggcc
    14641 ttcagccaca ggatgtagaa ctgacagtga cattacctgt aaccgaattc tttgataatg
    14701 acaatcaacc aaatgaagaa agaatagaaa gaaaaaaggc aaatgttctc cgtgaga
//
LOCUS       DAZUGK010000109        14726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZUGK010000109 DAZUGK010000000
VERSION     DAZUGK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.110"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHY5W_002922"
     CDS             complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHY5W_002922"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFS4447789.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHY5W_002923"
     CDS             complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHY5W_002923"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFS4447790.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHY5W_002924"
     CDS             complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHY5W_002924"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFS4447791.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHY5W_002925"
     CDS             complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHY5W_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4447792.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHY5W_002926"
     CDS             complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHY5W_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFS4447793.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTRLVQKSSSLPSSDIY"
     gene            complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHY5W_002927"
     CDS             complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHY5W_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFS4447794.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5174..6916)
                     /locus_tag="ACHY5W_002928"
     CDS             complement(5174..6916)
                     /locus_tag="ACHY5W_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFS4447795.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKDELAAAAAAAAGTTPAVGTDGVTKYTVDAG
                     LNKATAANVFANLADGAVVNASISNGFGAAATDYTYNKATNDFTFNASIAAAAVGDSN
                     SAALQSFLTPKAGDTANLSVKIGTTSVDVVLASDGKITAKDGSELFIDVDGNLTQNNA
                     GTVKAATLDALTKNWHTTGTPDAVSTVITTEDETTFTLAGGTDATTSGTITVANARMS
                     AESLQSATKSTGFTVDVGTTGTSAGDIKVDSKGIVQQHTGTGFEDAYTKADGSLTTDN
                     TTNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRS
                     SLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAK
                     ANQVPQQVLSLLQG"
     gene            7182..8588
                     /gene="fliD"
                     /locus_tag="ACHY5W_002929"
     CDS             7182..8588
                     /gene="fliD"
                     /locus_tag="ACHY5W_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFS4447796.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8613..9023
                     /gene="fliS"
                     /locus_tag="ACHY5W_002930"
     CDS             8613..9023
                     /gene="fliS"
                     /locus_tag="ACHY5W_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS4447797.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9023..9388
                     /gene="fliT"
                     /locus_tag="ACHY5W_002931"
     CDS             9023..9388
                     /gene="fliT"
                     /locus_tag="ACHY5W_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFS4447798.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9466..10953
                     /gene="amyA"
                     /locus_tag="ACHY5W_002932"
     CDS             9466..10953
                     /gene="amyA"
                     /locus_tag="ACHY5W_002932"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS4447799.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALIRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYIFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNNYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEFPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10987..11400)
                     /gene="yedD"
                     /locus_tag="ACHY5W_002933"
     CDS             complement(10987..11400)
                     /gene="yedD"
                     /locus_tag="ACHY5W_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFS4447800.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11587..12792
                     /gene="yedE"
                     /locus_tag="ACHY5W_002934"
     CDS             11587..12792
                     /gene="yedE"
                     /locus_tag="ACHY5W_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFS4447801.1"
                     /translation="MSWQQFKHTWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12789..13022
                     /gene="yedF"
                     /locus_tag="ACHY5W_002935"
     CDS             12789..13022
                     /gene="yedF"
                     /locus_tag="ACHY5W_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFS4447802.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13131..13799
                     /gene="yedK"
                     /locus_tag="ACHY5W_002936"
     CDS             13131..13799
                     /gene="yedK"
                     /locus_tag="ACHY5W_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFS4447803.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAAL
                     IQPV"
     gene            13949..14389
                     /gene="yedL"
                     /locus_tag="ACHY5W_002937"
     CDS             13949..14389
                     /gene="yedL"
                     /locus_tag="ACHY5W_002937"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4447804.1"
                     /translation="MQALVAYHISGMLQQSPPESSHALDVQKLRNPTVTFWSVWEGEQ
                     LAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHDRCLHRLSLETGTQ
                     AGFTACHQLYLKHGFVDCEPFADYRFDPHSRFLSLTLCENHELP"
     gene            complement(14527..>14726)
                     /locus_tag="ACHY5W_002938"
     CDS             complement(14527..>14726)
                     /locus_tag="ACHY5W_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFS4447805.1"
                     /translation="NLKLLFMECNPIKEAFLMPWALTGICYEISQRKYIVTNPDDYDK
                     YSDMVKKYVIDGEELIIKYYM"
BASE COUNT         3603 a         3788 c         3631 g         3704 t
ORIGIN      
        1 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
       61 gttttatcac gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt
      121 tcttattatt gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctcgggatcg
      181 gcaaataacg cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc
      241 cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt
      301 tcctgcgcca gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgct
      361 gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgca
      421 acacgctgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc
      481 ccaacttttg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
      541 acgatcaccg gcccttcaat aatgttctcc agcaccgtac gatgcggaaa caaattaaag
      601 ttctggaaaa caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
      661 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt
      721 tccagcagat ttatgctacg tagcaacgtg gttttgccgg aaccactcgg accgataatt
      781 gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtgg
      841 aattttttca ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat
      901 taagttgatt ctcaaaatgg ttctgcaacg tcgataacac agtcgccatg atccagtaga
      961 tgagcgaagc cgccagatac atggtgaaca cctccagcgt acgcgaggta atcaactgcg
     1021 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc
     1081 tgataaagct gttcgacagc ggcggcaacg ccacacgcgc cgcctgtggc aaaatcgcgc
     1141 gacgcatggt ctgccacggc gtcataccaa tactggctgc cgcttcccac tgacctttat
     1201 caatagaaga aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcaacgaca
     1261 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat
     1321 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
     1381 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca
     1441 gtaagccaaa aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca
     1501 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
     1561 aacgtgggga tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg
     1621 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agacccgcca tcgcttttcc
     1681 ggtatacaca ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat
     1741 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
     1801 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg
     1861 caattgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
     1921 gtgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgaccaccac
     1981 cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata
     2041 acccagcgcg cccagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
     2101 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca
     2161 catttcaatc tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt
     2221 ttctgcggtt gtgccaatag gattttccag cagcgccacg cagtgcagtc cgagtttcgc
     2281 agcgactgcg gcagtctggc gcacatggtt agactggatc gccccggcag taatcagcgt
     2341 atcggcacct tcgcgcagag catctgctgc gagaaattcc agcttacgta atttattgcc
     2401 gcccattgcc atgggtgtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc
     2461 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca gccgtggaaa
     2521 acgggttaaa ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat
     2581 tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat
     2641 tatgctgatt atttggtcac atcagcacca aaccattttt cggaaagggc ttgcaaagtg
     2701 ccgtcttttt gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct
     2761 ttacgcagcg ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta
     2821 tcgttggttt tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata
     2881 cggcctacgc gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgaca
     2941 ccctgaacat tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt
     3001 ttgcctttca gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc
     3061 tgaataccag aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcatcagaa
     3121 atggtgacct ggttaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg
     3181 tcccatttgg tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca
     3241 aattccactt caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa
     3301 gttccttcca gccctaccag tagcgtgccg cgctctttga ctttattaag cagaccttca
     3361 tctgcaaaac ttttaacgct catgcccgca accagcgcca cggccatcac acccatcaat
     3421 gcctgacgtc ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgtttgc
     3481 agtgtagagc cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa
     3541 tatatatcag aagaaggcag gctggaggat ttctgcacaa gtctggtaca ggtttggcgc
     3601 tgatagtgtt gatacttccg taacgcgatg cggtaattgt tggtgctggt ggtgggcagc
     3661 cacggggcga ggatttcatc aaggaaaccg tcctgcagct gatcgaggga aatattttgc
     3721 tcgtgtagat gattccccaa acggcgcaaa cgaacgacat attcacgcac cgtacctggg
     3781 ctcatttcag tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc
     3841 tgcgttttgc aatgtaaatc accgcaaaat cggcacaatg ccgcccatga tttttgttcc
     3901 gtttgccagc cattttcatc aagcagcgtg tccaggcggg aaatctcgat tttattcact
     3961 attttgccgt cgcgaactaa ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg
     4021 gtctggctgt gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc
     4081 accatcatta agaactcctg gtagtcaaag ttaaagtgcg gcatttactg acgttataac
     4141 ttacccagtt tagtgcgtaa ccgtttaata gcctggctgt gtaactgact gacccgcgat
     4201 tccccgacct ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg
     4261 gttaatacca gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctggcgc
     4321 agattactgt ccagtagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt
     4381 tcgatgctat cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg
     4441 gtgtcgagca acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca
     4501 gtttccgtgg cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca
     4561 cgcgcgttgc gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata
     4621 gcgccacgga tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag
     4681 cgttcgacgg cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg
     4741 ctcgcgggca gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc
     4801 tgccacagcg agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac
     4861 agccctgcgt tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga
     4921 cgattagtgg gtgaaatgag gggttatttg ggggttacca gtaaattgcg aacagaaaaa
     4981 acccccgccg gtggcgggga agcacgttgc tgacaaattg cgctttatgt tgccggatgc
     5041 ggcgtaaacg ccttacccgg cctacaaaaa tgtgcaaatt caataaattg caattcacct
     5101 tgtaggcctg ataagcgcag cgcatcaggc aatttggcgt tgccgtcagt ctcagttaat
     5161 caggttacga cgattaaccc tgcagcagag acagaacctg ctgcggtacc tggttagctt
     5221 ttgccagcac ggagttaccg gcctgctgga tgatctgcgc tttcgacatg ttggacactt
     5281 cggtcgcata gtcggcgtcc tgaatacggg actgcgcttc agacaggtta gtagtggtgt
     5341 tgttcaggtt ggtgactgcg gaatccagac ggttttgcac cgcaccgagg gaagaacgga
     5401 atttgtcgat ggagctgatc gcttcgtcca gagctttcag gggatcggcg gtggttgcag
     5461 ctttagtttc agcgtcagta gtaaaattac catccgctga aacatagact gctttacctg
     5521 aaccattggt cacagttccg tctttttgca aaaacagatt ggttgtatta tcggtagtca
     5581 gtgaaccatc agctttggtg taagcgtctt caaaacctgt acctgtgtgt tgttgtacta
     5641 tacctttact atcaacttta atatcgcctg cgctggtacc agtagttcca acatcaaccg
     5701 tgaatcctgt ggacttagtt gccgattgaa gagactcagc actcattctt gcatttgcta
     5761 cagtgattgt accagaagta gtagcatcag taccgccagc cagagtgaag gttgtttcat
     5821 cttcagttgt aattaccgta gatacggcat ccggtgtgcc tgttgtatgc cagtttttag
     5881 tcagtgcatc aagagtggct gctttgacag tcccagcatt gttttgagtg aggttaccat
     5941 ctacgtcaat aaatagttct gaaccatctt tcgcggtaat tttgccatcg ctcgccagaa
     6001 caacatcaac agatgtcgta ccgattttga cgctcaggtt agctgtatca cctgcttttg
     6061 gtgtcaggaa ggattgcaga gctgcgctgt tactatcacc gaccgcagca gcagcaatgc
     6121 tggcattgaa agtgaaatca tttgtagctt tattgtaggt gtagtctgta gctgctgcac
     6181 caaaaccgtt ggaaatgcta gcattaacaa cagcaccatc tgcaaggttt gcaaacacgt
     6241 ttgctgctgt ggctttgtta agccctgcgt ctacggtata tttggtcacg ccgtcagtac
     6301 cgacagcagg agttgtaccc gcagcagcag cagcagcagc agccagttca tctttagtcg
     6361 ctgcagtatt agccacagcc ccgccaccat tcacattaaa cccactcagc cccagcgtat
     6421 ctgagtcaat tttcttcaga tcaatagtga tagtctggcc gtcattcgca ccaacctgaa
     6481 ttttcattga accgtctttc gccagtacgt tcacgccgtt gaactgggtc tggccagata
     6541 cgcggtcaat ttcgtccaga cgggatttga tttcgtcctg aatggagtcc agatccgaat
     6601 cggagttagt cccggtagaa gcctgaaccg tcagttcacg gatacgctgt aagttgttgt
     6661 tgatttcgga cagcgcgcct tcggtggtct gtgcaacaga aataccgtcg ttggcgttac
     6721 gtgcagcctg agtcaggcct ttaatattag aggtgaaacg gttagcaatc gcctgacctg
     6781 cggcgtcatc cttcgcgctg ttaatacgca agccagaaga cagacgctcg atagaactcg
     6841 acagcgcaga ctggttcttg ttgatattat tttgagtgat cagcgagagg ctgttggtat
     6901 taatgacttg tgccatgatt cgttatccta tattgcaagt cgttgattac gtattgggtt
     6961 tccacccgtc ggctcaatcg ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag
     7021 aatttttttc acaaacagcc attttttgtt agtcgccgaa atactctttt ctctgcccct
     7081 tattcccgct attaaaaaaa acaattaaac gtaaactttg cgcaattcag gccgataacc
     7141 ccggtattcg ttttacgtgt cgaaagataa aaggaaatcg catggcaagt atttcatcgc
     7201 tgggagtcgg gtcaggtctg gatttaagtt caatcctcga tagcctcacc gccgcgcaaa
     7261 aagcgacgct aacccctata tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct
     7321 acggtacgct gaaaagcgcg ctgacgactt tccagaccgc caatactgca ttgtctaaag
     7381 ccgatctttt ttccgccacc agcaccacca gcagcaccac cgcgttcagt gccaccactg
     7441 cgggaaacgc tatcgccggg aaatacacca tcagcgtcac ccatctggcg caggcgcaaa
     7501 ccctgaccac gcgcaccacc agagacgata cgaaaacggc gatcgccacc agcgacagta
     7561 aactcaccat tcaacaaggc ggcgacaaag atccgattac cattgatatc agcgcggcta
     7621 actcgtcgtt aagcgggatc cgtgatgcca tcaacaacgc aaaagcgggc gtaagtgcga
     7681 gcatcattaa cgtgggtaac ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc
     7741 ttgataatgc gatgacactc tcggtcagcg gtgatgatgc gctacaaagt tttatgggct
     7801 atgacgccag tgccagcagc aacggtatgg aggtctcggt cgccgcccag aatgcgcagc
     7861 tgacggtcaa caacgtcgcc attgagaaca gcagcaacac catcagcgac gcgctggaaa
     7921 acatcaccct gaacctgaac gatgtcacca cgggcaacca gacgctaacc atcactcagg
     7981 acacctccaa agcgcaaaca gcgattaaag actgggtgaa tgcctataac tcgctaatag
     8041 ataccttcag cagcctgacc aaatacaccg ccgtagatgc gggagctgat agccagagtt
     8101 ctagcaatgg cgcactgctc ggcgactcca cgttgcggac gattcagaca cagttgaagt
     8161 cgatgctgag taataccgtc agttcttcca actataaaac gctggcgcag attggtatca
     8221 cgaccgatcc cagcgatggc aaactggaac tggatgccga caaactcacc gctgcactga
     8281 aaaaagatgc cagcggcgta ggtgcattga ttgttggcga tggtaaaaaa accggcatca
     8341 cgaccaccat cggcagcaac ctgaccagtt ggctttcgac aacgggcatt attaaagccg
     8401 ctaccgatgg cgttagtaag accctgaata agttaactaa agactacaac gccgccagcg
     8461 atcgcattga tgcgcaggtc gctcgataca aagaacaatt tacccaactg gacgttttaa
     8521 tgacctcgtt aaacagcacc agcagctact taacgcagca gttcgaaaac aacagtaatt
     8581 ccaagtaatc attattcatc gggagacagg tcatgtacgc ggcaaaaggc acccaggcct
     8641 atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca
     8701 tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca
     8761 atcagcaagg caaaggcgtc tctttatcaa aagcgatcaa catcattgag aacggactgc
     8821 gggtgagtct tgatgaagag agcaaagacg aattaaccca aaacttgatt gctctttata
     8881 gctatatggt caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag
     8941 aagtggaagc attaatgcgc aatattgccg atgcctggaa agagtcgtta ctctcccctt
     9001 ctttgattca ggacccagtc tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca
     9061 actcgtcgaa aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact
     9121 catcgccagc gaaatggcgt atgttaatgc ggtgcaggag attgcacatt tgactgaaga
     9181 ggttgacccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga
     9241 caacgaaagc aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt
     9301 cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt
     9361 gctggctccg caggataacc tcttttgaat ctgaatgagt cgatggctcg cgaataatcc
     9421 gattacggct acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg
     9481 ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc
     9541 gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat
     9601 aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggt
     9661 gagtttgatc agaaaggcag catccctact aaatatggcg ataaagtaca actgctggcc
     9721 gccattgacg ccctgatacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac
     9781 aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt
     9841 acgcaaattg atgaagaaat cattgagtgc gaaggctgga cgcgttacat cttccccgcc
     9901 cgtgccgggc aatactcaca gtttatctgg gatttcaaat gttttagcgg tatcgaccat
     9961 atcgaaaacc ctgatgaaga tggcatattt aaaattgtta acaactacac cggcgaaggc
    10021 tggaacgatc aggttgatga tgaattaggt aatttcgatt atctgatggg tgagaatatc
    10081 gatttccgca atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa
    10141 caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccagc ctggttttat
    10201 aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcagag
    10261 tactggtcgc atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc
    10321 atgctgttcg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac
    10381 tacgacatga cgcagatttt cacgggtaca ttagttgaag ccgatccttt ccatgccgtg
    10441 accctcgttg ccaaccacga cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg
    10501 tggtttaaac cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg
    10561 ttctatccgg acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat
    10621 ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc
    10681 gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt
    10741 ggcaccgacg aatttcccgg ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa
    10801 accattcatt taggagagaa ttacggcaac aaaacctggc gtgattttct tgggaaccgg
    10861 caagagagtg tagtaaccga cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc
    10921 gtcagcgtgt gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag
    10981 atttcattac ggcaatggcg tcggtaacgg cgctttatcc agcgcagcgg cacactctgc
    11041 cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg
    11101 ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt
    11161 caacttgccc ggcacagcaa tcacccgctg ccactgacgg caatcaagag tatcgccctc
    11221 tttagtgaca atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt
    11281 ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt
    11341 ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat
    11401 cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc
    11461 tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa
    11521 ataaatttgc atggtgtatg attcgcgact cttgtttcaa accttctgag ttcagaggct
    11581 acattcatgt catggcagca attcaaacac acctggttga ttaagttctg ggcacccatc
    11641 cctgcggtca tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt
    11701 tgggctgtca cgggtgaatt tacccgttgg ggcggccagc tcctgcaact gttcggcgtc
    11761 catgctgaag agtggggtta ctttaaaatt atccatctgg aaggatcgcc attaacccgc
    11821 atcgacggga tgatgatcct cggtatgttt ggcggctgct ttgccgcagc gctgtgggcc
    11881 aacaatgtca aactgcgaat gccgcgcagc cgtatccgca ttatgcaggc catcattggc
    11941 ggcattatcg ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt
    12001 accggtattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt
    12061 tcatggtttg gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag
    12121 aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt
    12181 ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc tgctcacagc gatgaaccaa
    12241 cccaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg
    12301 caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg
    12361 aaagcaatca ttattggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta
    12421 ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt
    12481 ggttttggca tcgtgctggc tggcggctgc gaaaccggct ggatgtaccg cgcggtagaa
    12541 ggccaggtgc actactggtg ggtcggtctg ggcaatgtga tcggctcaac gattctggcg
    12601 tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg
    12661 aaaacctttg gtccgatggg tggcctgctg gtgacatatt tgctgttgtt tgctgcgctg
    12721 atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag
    12781 gagatcgcat gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc
    12841 cttatccggc agtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg
    12901 aagtggtgag cgactgtccg cagtcgatca ataatattcc actggatgcg cgtaatcacg
    12961 gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt
    13021 aatccctcat tcccgctgtg gcccacagcg ggaatatcgt tgcctctgta ccacatagat
    13081 tttacccgcc ccctctccta ttcttttcag tgtgctctca ggaggtttct atgtgtggac
    13141 gctttgccca atcccaaacg cgtgaagatt accttgcgct tctcgcggaa gatattgaac
    13201 gcgatattcc ctatgatccc gaacccattg gcagatacaa cgtcgcgccg ggaaccaaag
    13261 tccttctgct cagtgaacgt gacgaacacc ttcatttgga tccggttttc tggggatatg
    13321 ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gccgcaacca
    13381 gtcgcatgtt taagccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
    13441 ttgagtggaa aaaagaaggc gacaaaaagc agccttattt tatctatcgt gctgatggac
    13501 aacctgtttt tatggcagcg ataggcagca caccgttcga gcgcggtgac gaagccgaag
    13561 gatttttgat tgtcactgca gcagcagacc agggtctggt agatattcat gaccgccgcc
    13621 cgctggttct gtcgccagaa gctgcgcggg aatggatgcg gcaagatatt ggcggtaaag
    13681 aagcctcaga aatcgctacc agtggttgtg tgccagcaaa ccagttcacc tggcacccgg
    13741 tatcgcgcgc ggtgggtaat gttaaaaacc agggcgcagc gttaattcaa cctgtttgat
    13801 aagcctggag attattgatc aacaatactg cgtcataaga aatctctatt agacaaagat
    13861 ttcattacct gttggcatat tgcaaaaata acaccaatac ggaatcgtca tgttcacgat
    13921 ttaaacagat gatctcaccc atccagcagt gcaagcatta gtggcttacc atatttccgg
    13981 catgctgcag cagtctcccc ccgaaagcag tcatgcttta gatgtgcaaa aattacgtaa
    14041 cccgacagtg acattctggt cagtatggga aggcgaacaa ctcgcaggaa ttggtgcgct
    14101 gaagttgctg gatgataaac atggcgagct gaaatcaatg cgtaccgcgc caaattattt
    14161 acgtcgtggt gtcgccagtc tgattttacg ccacattttg caggtcgccc atgacagatg
    14221 ccttcatcgc ctgagcttag aaacgggtac acaggctgga tttacggcct gccatcaact
    14281 ttatttgaaa catggattcg ttgattgcga accgtttgct gattatcgat ttgatccaca
    14341 cagtcgattt ttatcattga cgctatgcga aaatcatgaa ttgccatgag ccagacgcag
    14401 cacattcttg cattcgacgt gctgcgcctt tatttatcac cagcaggaaa cgccttgtcc
    14461 atagacgcca cttctacatg cgccacaaga aacctctatt ccagtgacac aattacgcct
    14521 aattaattac atataatatt taattatgag ctcctcacca tctattacat actttttaac
    14581 catgtcggaa tatttatcat aatcatcggg gttcgtaaca atatattttc gctgcgatat
    14641 ttcatagcaa atccctgtca gagcccatgg cattaaaaat gcctctttaa tgggattgca
    14701 ttccataaaa agtaatttta aattgc
//
LOCUS       DAZUGK010000110        14697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZUGK010000110 DAZUGK010000000
VERSION     DAZUGK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.111"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            104..307
                     /gene="aaeX"
                     /locus_tag="ACHY5W_002939"
     CDS             104..307
                     /gene="aaeX"
                     /locus_tag="ACHY5W_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFS4447806.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            315..1247
                     /gene="aaeA"
                     /locus_tag="ACHY5W_002940"
     CDS             315..1247
                     /gene="aaeA"
                     /locus_tag="ACHY5W_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFS4447807.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1253..3220
                     /gene="aaeB"
                     /locus_tag="ACHY5W_002941"
     CDS             1253..3220
                     /gene="aaeB"
                     /locus_tag="ACHY5W_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFS4447808.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3312..3584
                     /gene="yhcO"
                     /locus_tag="ACHY5W_002942"
     CDS             3312..3584
                     /gene="yhcO"
                     /locus_tag="ACHY5W_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFS4447809.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGQLRFNVRH"
     gene            complement(3640..3903)
                     /gene="yhcN"
                     /locus_tag="ACHY5W_002943"
     CDS             complement(3640..3903)
                     /gene="yhcN"
                     /locus_tag="ACHY5W_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFS4447810.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4268..4738)
                     /gene="argR"
                     /locus_tag="ACHY5W_002944"
     CDS             complement(4268..4738)
                     /gene="argR"
                     /locus_tag="ACHY5W_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFS4447811.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5173..6111
                     /gene="mdh"
                     /locus_tag="ACHY5W_002945"
     CDS             5173..6111
                     /gene="mdh"
                     /locus_tag="ACHY5W_002945"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS4447812.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(6174..7241)
                     /gene="degS"
                     /locus_tag="ACHY5W_002946"
     CDS             complement(6174..7241)
                     /gene="degS"
                     /locus_tag="ACHY5W_002946"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFS4447813.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(7331..8698)
                     /gene="degQ"
                     /locus_tag="ACHY5W_002947"
     CDS             complement(7331..8698)
                     /gene="degQ"
                     /locus_tag="ACHY5W_002947"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFS4447814.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(8852..9250)
                     /gene="zapG"
                     /locus_tag="ACHY5W_002948"
     CDS             complement(8852..9250)
                     /gene="zapG"
                     /locus_tag="ACHY5W_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFS4447815.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9444..10571
                     /gene="zapE"
                     /locus_tag="ACHY5W_002949"
     CDS             9444..10571
                     /gene="zapE"
                     /locus_tag="ACHY5W_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFS4447816.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            10790..11218
                     /gene="rplM"
                     /locus_tag="ACHY5W_002950"
     CDS             10790..11218
                     /gene="rplM"
                     /locus_tag="ACHY5W_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4447817.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            11234..11626
                     /gene="rpsI"
                     /locus_tag="ACHY5W_002951"
     CDS             11234..11626
                     /gene="rpsI"
                     /locus_tag="ACHY5W_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4447818.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            12021..12659
                     /gene="sspA"
                     /locus_tag="ACHY5W_002952"
     CDS             12021..12659
                     /gene="sspA"
                     /locus_tag="ACHY5W_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFS4447819.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            12665..13162
                     /gene="sspB"
                     /locus_tag="ACHY5W_002953"
     CDS             12665..13162
                     /gene="sspB"
                     /locus_tag="ACHY5W_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFS4447820.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(13204..14571)
                     /gene="dcuC"
                     /locus_tag="ACHY5W_002954"
     CDS             complement(13204..14571)
                     /gene="dcuC"
                     /locus_tag="ACHY5W_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS4447821.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSSYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVTSCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         3603 a         3631 c         3778 g         3685 t
ORIGIN      
        1 aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg cgacagatgc
       61 tgaaaataat aacgcctgct ctctctatac aaccaaggtc aacatgagtc tgtttcccgt
      121 tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt tatcactggc
      181 gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact ttgtctggca
      241 tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat cgcgactgtt
      301 cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc atcacggtcg
      361 tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac accgaatccc
      421 cgtggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg gacgtttctg
      481 gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga caggtactgt
      541 tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt
      601 attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg
      661 tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa acggttctgc
      721 atcagttagc gaaagcgcaa gcgacccgcg atctggcaaa actggatctt gaacgcacgg
      781 tgatccgcgc gccagcggat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta
      841 ttactcgtgg atcaacggcg gttgcgctgg tgaaacagaa ctctttctat gtattggcct
      901 atatggaaga aactaagctg gaaggggtgc gtccggggta tcgtgcagag atcacgccgc
      961 ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgcaggg gtcaccaacg
     1021 ccagcagcac gcgtgacgac aaagggatgg cgactatcga ctctaacctt gaatgggtgc
     1081 gtcttgcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc
     1141 ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg
     1201 attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat
     1261 tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact
     1321 ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc
     1381 ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat
     1441 tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt
     1501 gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc
     1561 cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtatg cgtgggggct
     1621 ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat tgcttacgcc
     1681 gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg cgattatggc
     1741 ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc tggaaagttt
     1801 gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt
     1861 cgataaagcc tggggcgacc tggtgcgacg caccacggcg ctacaaggca tgcgcagcaa
     1921 cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac
     1981 gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc
     2041 ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga
     2101 cgtccacaag cagctcaaac gcctgcgaag agttatcgcc tggaccgggg aacgggaaac
     2161 gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg
     2221 cgttattagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga
     2281 agtcaaagta gagtcagccg aacgtcatca tgcgatggtt aacttctggc gaaccacact
     2341 ttcctgcatt ctgggaacgc ttttctggct gtggacgggc tggacttccg gcagtggtgc
     2401 aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt
     2461 ggcgatcgac tttatctacg ggacgctggc cgcgctgccg ttagggctgc tctacttttt
     2521 ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc tggcagtgct
     2581 gggattcttc ctcggaatag aagtacagaa acggcgactg ggctcgatgg gggcgctggc
     2641 cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct
     2701 cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt
     2761 gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc
     2821 tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta
     2881 tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc
     2941 gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga
     3001 tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag
     3061 cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaacttgaaa tctaccagga
     3121 aaagttacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc ggctggcggg
     3181 gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag
     3241 cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc gaaacgtgac
     3301 ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt caggaggatt
     3361 tttatcgcga cttcagccaa gcctttggtc tggcgaaaga taaggtacgc gatctcgact
     3421 cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc
     3481 atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag
     3541 aggaagagct ggaagggcaa ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc
     3601 cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca gttcagccgt
     3661 agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc
     3721 ttccgctttt ttgttcagca tttcacgtat atccattggc gaagacgcca caccacttac
     3781 ggatacggtc ccgattgctt cacggttttg tgcttgtgca gcatcaatgg agtcggcagc
     3841 gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gcaacagtgg ttttgatttt
     3901 catgattttt acctcgacat aatctttttg ctgggtcttt gtttcgtgac ccttatcaca
     3961 aaatcaagta tacactaatt attgggctaa ttaataccac gctaactgtt tctgttggta
     4021 ctaagtgtta aaaatgtgaa ttttaataac aaaaaagaat taaaaatacg gttatttatc
     4081 gttaattcta gtgttttcaa ttagataatg aattttgcat aatgtgcttt ttttaccgta
     4141 tgtattcatt gtgtgaatga catgtcgcag taaaacgcac tattcgttaa attaatcgca
     4201 ggggaaagca gggggttgag agggataagc aacattttcc ccgccgccag aagcgacggg
     4261 gcagagatta aagctcctgg tcgaacagct ctaaaatcgc ttcgtacagg tctttgacgg
     4321 tgaaaccgtt agcaggggtg gtaaagatgg tgtcatcgcc agcgatggtg cccagaatac
     4381 cttctgcttt gcccagtgag tccagcaggc gagcaattaa ctgcgccgcg ccagggctgg
     4441 tatgaatcac gacaactgca tcgttgtagt cgatatccag caccagattc ttcaatggac
     4501 tggaggtggt tggtacaccc agttcagctg gcaggcagta aaccatctcc attttggcat
     4561 tgcgtgtacg tacagcacca aacttggtca acatccgcga gactttagac tgattaatat
     4621 tgtcaaagcc ttgctcctgc aacgcggcga cgatttcgcc ctgggagcta aatttctctt
     4681 ctttaagtaa tgctttaaat gctttaacta gttcttcttg cttagccgag cttcgcataa
     4741 gtcacccgat atggtggttg atacaacatt attgtgcata cagatgaatt tttatgcaaa
     4801 cagtcagccc tgaagaaggc tgaaataatg ttatgaaaga gcgggatttt atcaaatttc
     4861 gttattgaga aacatgcctg cgtcacggca tgcaaattct gcttaaaagt aaattaattg
     4921 ttatcaaatt gatgttgttt tggctgaacg gtagggtata ttgtcaccac ctgttggaat
     4981 gttgcgctaa tgcataagcg actgttaatt acgtaagtta ggttcctgat tacggcaatt
     5041 aaatgcataa acgctaaact tgcgtgacta cacattcttg agatgtggtc attgtaaacg
     5101 gcaattttgt ggattaaggt cgcggcagcg gagcaacata tcttagttta tcaatataat
     5161 aaggagttta ggatgaaagt cgcagtcctc ggcgctgctg gcggtattgg ccaggcgctt
     5221 gcactactgt taaaaaccca actgccttca ggttcagaac tctctctgta tgatatcgct
     5281 ccagtgactc ccggtgtggc cgtcgatctg agccatatcc ctactgctgt gaaaatcaaa
     5341 ggtttttctg gtgaagatgc gactccggcg ctggaaggcg cagatgtcgt tcttatctct
     5401 gcaggtgtag cgcgtaaacc gggtatggat cgttccgacc tgtttaacgt taacgccggc
     5461 atcgtgaaaa acctggtaca gcaagttgcg aaaacctgcc cgaaagcgtg cattggtatt
     5521 atcactaacc cggttaacac cactgttgca attgctgctg aagtgctgaa aaaagccggt
     5581 gtttatgaca aaaacaaact gttcggcgtt accacgctgg atatcattcg ttccaacacc
     5641 tttgttgcgg aactgaaagg caaacagcca ggcgaagttg aagtgccggt tattggcggt
     5701 cactctggtg ttaccattct gccgctgttg tcacaggttc ctggcgttag ttttaccgag
     5761 caggaagtgg ctgatctgac caaacgtatc cagaacgcgg gtactgaggt ggttgaagcg
     5821 aaagccggtg gcgggtctgc aaccctgtct atgggccagg cagctgcacg ttttggtctg
     5881 tctctggttc gtgcactgca gggcgaacaa ggcgttgtcg aatgtgccta cgttgaaggc
     5941 gacggtcagt acgcccgttt cttctctcaa ccgctgctgc tgggtaaaaa cggcgtggaa
     6001 gagcgtaaat ctatcggtac cctgagcgca tttgaacaga acgcgctgga aggtatgctg
     6061 gatacgctca agaaagatat cgccctgggc gaagagttcg ttaataagta attgattagc
     6121 gaataataaa aaaccggagc acagactccg gttttttgtt ttgagcactc gacttaattg
     6181 gttgccggat attcctgaat ggtgacctgc agcgttaact gcttatcatc acgcatcact
     6241 acaaccggga tcaccgaacc agggcgaatt tctgccacct gatccatcgt ctccagagca
     6301 gagatggccg gtttgttatc caccgaaata atcagatcgt tgacctgaat acccgcattc
     6361 gccgccgggc cgtcaggtga cacttcatta accacgatcc cttgcagttg atctatacca
     6421 ccgccctgcg cgtgcagtgg tgcgatctcg cgtccgccga taccaatgta gccgcggatc
     6481 acgcgaccat cgcggatcag cttatccata attttggttg ctaactggaa aggaatcgca
     6541 aagccgatac cttccggcgt ttcgccatcg ttactcttat caaacgacag cgtgttaatg
     6601 cccatcagtt cgcccagcga gttcaccagc gcgccgccag agttaccgtg gttaatggaa
     6661 gcatcggttt gtaggaagtt ttgccgcccg gtcgggttca gaccgattcg acccgtggca
     6721 ctaataatcc cctgggtaat ggtctgcccg aggttgtacg ggttaccgat cgccagtacc
     6781 acgtcgccaa tgtgcggtac gcgacgtgca ttaattggaa tggtaggtaa accgccagtg
     6841 gcattaattt taagtaccgc cagatcggtt agagagtcag atcccaccag caatgcttca
     6901 aatacacgtc catcctgtaa ggcgacgatg atctgatcgg cgtcgttgat gacgtgttta
     6961 ttggtgatga tataaccgcg ttgatccatg attacaccgg atcccagggt gcggatctca
     7021 agctggttgt gagagttggt gttcaaacca cggttgtaaa cgttaaccac cgctggcgcg
     7081 gcgcggcgaa ccgccagatt ataactggca ggcgtctcat cggtactgtc aaattgcgga
     7141 gtggaaagcg ggttaaggct gcgcagcgaa ggcatggcaa ccagcagaat agcgccgaca
     7201 attaatccaa tcgcaacgga acgtaagagc ttcacaaaca tgatggaggc gtcattaaaa
     7261 aagggaacgg cagcagcata ccacgagtta accggacatc acacgtcagc ctgatgcccg
     7321 gtttacgaca ttaacgcatc agcagataga tgctttcatt gccgcgtaca atttgcaggg
     7381 cgatgatggc cggttttgcc gccagcactt tacgcatttc agcaatcgag ttcacccgat
     7441 cgcggttgac gccaatgatc acatcgtctt tttgcaagcc agcctgagca gctgggcttc
     7501 ctttgacaac ttcatcgatt ttaatacctt tgccgccatc ttttagctga ccatcgctca
     7561 acgttgcacc ttccagcgct ggcgtgatca tttcagcgct ggccgacgaa gaggtgctgg
     7621 tatcgagcgt cacttctact tccagtggtt tgccgttacg cagcaggcca agcttcactt
     7681 tcgtgcccgg ctcggtggtc gcgatacgag agcgcaactc agcaaagcta ttcagcggtt
     7741 tgccgttgag gctggtaata atatcgcccg ctttgacgcc cgctttcgcc gagccagaac
     7801 ctggcaacac ttcgctgaca aacgcgccac gctgcacgtc aaggttgaag gctttggcga
     7861 tatcggcact catctcggtg cctttgatgc ctaacaaacc gcgtttgatt tcaccaaagt
     7921 cgataagctg ctgcgccagt gttcgcgcca tattactggg gatggcaaat ccaattccga
     7981 cgctcccgcc gccaggcgca aggattgcag tgttgatgcc aattaactca ccgttaaggt
     8041 ttaacagtgc accgccggag ttaccgcggt taatggaagc atctgtctgg ataaagtttt
     8101 ccagaccttc aagattcaac ccgctgcgtc ctaatgcgga aacaatgcca gaggtggcgg
     8161 tttgcccaag gccaaatggg ttaccgaccg ccacggcaaa atcaccgacg cgcaatttat
     8221 cggagtcggc aatagcgatt tgcgttaatt tgctcgggtt ttgaatttgc aacagggcga
     8281 tatcgctctg gtcatcgcta ccaatcagtt ttgcatcaaa ctcgcgccca tcattgagct
     8341 gaatactgat tttctgtgcc tgattaatca catggttgtt ggtcagcaca tagcctttac
     8401 tggcgttgat gatgacaccg gagcctaaac cttcgaaggg ttgtgcaggt tgatccggta
     8461 aatcatcacc aaaaaacttt ttgaattctt ccgggatttt ctgtccctga ctggccgttc
     8521 cttccacccg tacgctcacc actgccggaa gcactttttc cagcattgga gccagactgg
     8581 gaaggggggc ctgatcggca acctggcctg gaatcgacgc gacggcctga aatgacgccg
     8641 agagagttaa cccgacactt aacgctaatg cactcaacag ctgggtttgt tttttcatta
     8701 ttcctgctct cgtacctgaa tgataagaaa agagattcaa aacagttcga tgttattgaa
     8761 ttttcagacg gctaacaatg agacaccaga ttaaatgata gtcggggagg gagagaagag
     8821 gagggcgcaa tggctgcgcc cgaaaaataa attagtcgcg cttcgcgcca gtacgcagca
     8881 ggccggatgc gccttcagaa tagtcgcgag gcatctgcac cggtgcctga tcgttactgg
     8941 cttcagactc tgccagacga ttacggaacg ggtttgcttc tgcagacagt tccggcagca
     9001 ggctgctgga gcttttcgcc atgtgctgat acagctggcg atagtcgtgc gccatggtat
     9061 ccagtaattc cgcgctgcgg gcaaagtggc taaccagctc ttcgcgatac tcgtccagtt
     9121 cagctttatt cttttccagt tcgtactgca acgcctgttg ctggcgtagt ttacgattac
     9181 caaaacgcat ggccacagca ccaataatga tgccgacgac taacccaatt agcgcatatt
     9241 cccaggtcat gaacatctcc cgttgtcttt tgtttccgta gggtgttggc ttcaggctcc
     9301 atgcctgcgg ctgattatgc cactataacc gttaattcca cagaagtgga atcccgactg
     9361 catatcgcgt agtgtagaac ggcctttttt tcgtcaaccg tgaacaacgg cgcaccgatt
     9421 attcaaggaa taacaataag atcatgcaaa gcgttacccc aacatcgcaa tacctgaagg
     9481 cgctcaatga aggcagccat caacccgacg acgttcaaaa agaggccgtc agccgcctgg
     9541 aaattattta tcaggaactc atcaatagca cgccaccagc ccccaggacg agtgggctaa
     9601 tggcgcgggt cggtaagctg tggggtaaac gcgaagacac aaagcatacg ccagtgcgtg
     9661 gcttatatat gtggggcggt gtaggacgcg ggaaaacctg gctgatggac cttttctatc
     9721 aaagcctgcc gggagagcgg aaacagcgcc tgcactttca ccgttttatg ctgcgggtgc
     9781 acgaagagct aactgcctta caggggcaga ccgatccgct ggaaattatt gccgatcgct
     9841 ttaaagccga aactgacgtg ctctgttttg acgaattttt tgtttctgat attaccgacg
     9901 ccatgctact tggcggtctg atgaaagccc tgttcgcccg cggtattacc ctggtagcga
     9961 cgtcaaatat tccgccggat gaactttatc gaaatggcct gcaacgtgcg cgttttctgc
    10021 ctgcaatcga tgccattaaa cagcattgtg atgtaatgaa cgtggacgct ggtgttgatt
    10081 atcgactgcg tacactcact caggcgcatc tgtggctttc gccactcaac gatgaaatcc
    10141 gggcgcaaat ggataaacta tggttggcgc tggcgggggc gaaacgagaa atttcaccaa
    10201 cgttagaaat caaccatcgg ccattagcga caatgggcgt cgagaaccag acgctggcgg
    10261 tctcttttac tacgctgtgc gtcgacgccc gcagtcagca tgactatatt gcgctctcac
    10321 gtctctttca tacggtcatg ttgtttgatg taccagttat gacgcggttg atggagagcg
    10381 aagcgcggcg ctttattgcg ctggtggatg agttttacga gcgccatgtc aaattagtgg
    10441 tgagtgcaga agtgccgctg tatgaaattt atcagggcga tcggctgaag tttgagttcc
    10501 agcgttgcct gtcacgtctg caagagatgc aaagcgaaga gtatctgaag cgcgagcatt
    10561 tggcgggtta aaacctgtca caaatcacaa aaaggggtcg atctttgacc ccgacttctc
    10621 tataatcctg cgaccccacg ttacaagaaa gtttttttcc caaaactttt tgtgtgctgg
    10681 cataggctat tcgaaggggt aggtttgccg gactttgtcg tgtgaacctc aacaattgaa
    10741 gacgtttggg tgttcaccaa cgtgtaacta tttattgggt aagcttttaa tgaaaacttt
    10801 tacagctaaa ccagaaaccg taaaacgcga ctggtatgtt gttgacgcga ccggtaaaac
    10861 tctgggccgt ctggctactg aactggctcg tcgcctgcgc ggtaagcaca aagcggaata
    10921 cactccgcac gtagataccg gtgattacat catcgttctg aacgctgaca aagttgctgt
    10981 aaccggcaac aagcgtactg acaaagtgta ctatcaccac accggccaca tcggtggtat
    11041 caaacaagcg acctttgaag agatgattgc tcgccgtcct gagcgtgtga ttgaaatcgc
    11101 ggttaaaggc atgttgccaa aaggcccgct gggtcgtgct atgttccgta aactgaaagt
    11161 ttacgcgggt aacgagcaca accacgcggc acagcaaccg caagttcttg acatctaatc
    11221 gggattatag gcaatggctg aaaatcaata ctacggcact ggtcgccgca aaagttccgc
    11281 agctcgcgtt ttcatcaaac cgggcaacgg taaaatcgta atcaaccaac gttctctgga
    11341 acagtacttc ggtcgtgaaa ctgctcgcat ggtagttcgt cagccgctgg aactggtcga
    11401 catggttgag aaactggacc tgtacatcac cgttaaaggt ggtggtatct ctggtcaggc
    11461 tggtgcgatc cgtcacggta tcacccgcgc tctgatggaa tacgacgagt ccctgcgttc
    11521 tgaactgcgt aaagctggct tcgttactcg tgacgctcgt caggttgaac gtaagaaagt
    11581 cggtctgcgt aaagcacgtc gtcgtccgca gttctccaaa cgttaattgg cttctgctcc
    11641 ggcagaaaac aatttgcgaa aaaacccgct tcggcgggtt tttttatggt taaattctga
    11701 atcagcgtaa aaaatggaaa gttacttttt gctgccgtct gacagacagg caaaataaaa
    11761 accacatcgc caataaggga ctaagtcaac tatttcagac taaagcgcat ctctttttcc
    11821 ccatttccgg catcgactca ccacaaaggt cgcaaaatct ggtaaactat catccaattt
    11881 tctgcccaaa tgtcgggtat tgctcatttt ttgtttgatt ttcgaacaaa gagagtcgtt
    11941 ccttactggg taacacaact tctgactggc cacctggtgg ctggtagcag taaaaattct
    12001 gactatacct ggaggttttc atggctgtcg ctgccaacaa acgttcggta atgacgctgt
    12061 tttccggtcc tactgacatc tatagccatc aggtccgcat tgtgctggct gagaaaggtg
    12121 taagtttcga gatcgaacac gtggaaaagg acaatccgcc tcaggatctg attgacctca
    12181 acccgaatca gagcgttccg accctggtgg atcgtgagct gaccctgtgg gaatctcgca
    12241 tcattatgga atatctggat gagcgtttcc cgcatccgcc actgatgcct gtttacccgg
    12301 tagctcgcgg tgaaagccgt ctgtacatgc atcgcatcga aaaagactgg tacacgctga
    12361 tgaacaccat catcaacggt tcagcttctg aagcagatgc cgcacgtaag caactgcgcg
    12421 aagaactgct ggcgattgcg ccggtcttcg gtcagaagcc gtacttcctg agcgatgagt
    12481 tcagcctggt cgattgctac cttgctccgc tgctgtggcg tctgccgcaa ctgggcatcg
    12541 agttcagcgg cccgggtgcg aaagagctga aaggctatat gacccgcgtc tttgagcgtg
    12601 actctttcct tgcttcttta actgaagcag aacgtgaaat gcgtctgggc cggagttaat
    12661 ctgtatggat ttgtcacagc taacaccacg tcgtccctat ctgctgcgtg cattctatga
    12721 gtggttgctg gataaccagc tcacgccgca cctggtggtg gatgtgacgc tccctggcgt
    12781 gcaggttcct atggaatatg cgcgtgacgg gcaaatcgta ctcaacattg cgccgcgtgc
    12841 tgtcggcaat ctggaactgg cgaatgatga ggtgcgcttt aacgcgcgct ttggtggcat
    12901 tccgcgtcag gtttctgtgc cgctggctgc cgtgctggct atctacgccc gtgaaaatgg
    12961 cgctggcacg atgtttgaac ctgaagctgc ctacgatgaa gatgccagca tcatgaatga
    13021 tgaagaggca tcggcagaca acgaaaccgt tatgtcggtt attgatggcg acaagccaga
    13081 tcacgatgat gacactcatc ctgacgatga acctccgcag ccaccacgcg gtggtcgacc
    13141 ggcattacgc gttgttaagt aatacaaaac aggccctggt ggcctgtttt gtcttttaat
    13201 ggcttaataa aatagcgtga ttgtggcaat catattcacc acgaaaccga ctgccatggg
    13261 gatcgctgtc cgcttcacca ccgcaaaagg cgaaacgcct gcaattcctg acgtaacgac
    13321 taccaccgca gtaatcggcg aaaccgcgcg tgctagcgtc gtggcaaaat gcatcggcat
    13381 aatcattaca accgctggta catgtagtcc ggctgcgata ttcggaataa gactggcaaa
    13441 tgacataaac ggcgcattgc cagagcccat cacaatggca caaatggcaa tgaccagcgc
    13501 cataataatc atcacgccaa taccgcccag accagaatgc tccgcacccc tgataaccgc
    13561 atcgacagtg ccaatcgtcg ttaagccttt cgcaaatatt tcacccgcga ccaccagcgt
    13621 taccacgttg gcaaactgcg tacccatgcc gtcaaaaaac gcctgcacat cgtccattgt
    13681 ttcgcgcaag ttatgcttgc ggaagaactc aacaaacatc gtcaccgtca aactcagtaa
    13741 catcaccacc accagatgca gttctgactg catcagcccg atgtgggcaa ggaatagcga
    13801 gccgagcatc aggattaacg gcatcacagg taaaatggcg taatagagcg gcgggacatt
    13861 atcgagagct ttttgctctg cctgttcgtg attgatattt ttatcttttt tgtcaaaagc
    13921 gcgttgcacg aaaaagtggg agatcgccac cgagataatg acgcaagagg tgaccggaag
    13981 ctggtagtgg aagaagtaag tggcaatttt cattcccgcg acctgggctg caaaaatgga
    14041 gttcgtttcc agaatccccc actcaatgga catcgtggtt gcgataaccg ccaccgcaga
    14101 gaggcgactt actcccagac tcaccagcgt cggaaataag gtgaccatca gcaacatacc
    14161 cagaccggag gcgctggtaa taaactgcgc catgatttgg ccgatgacgt aagttgccga
    14221 cagaataata tacggcgagc gaatgagttt taacgggcgg cttaacaagc tcaccatcgc
    14281 gcgactggcc cctatgcgct ccatgtagcg ggcataaccg cccaccgcca taatcgacag
    14341 ccccaatccg gcaatgcggt tggacagcat gcgcaggatt tcattataaa tatcgacgat
    14401 caagtagctg ctgcttttgg tgggatcgag tacaccaccg aaccctaacc agacaccgca
    14461 catcatcagg aagatacctg cggcagccag caccacctga ggtttgtagt ttttcaggat
    14521 caaatagccc atcgtaatta atacgatgac agatataatt atgccgaaca ttgttgaact
    14581 ccgtgtcaaa agaaaacggt caatcccata aacggcagat tgaaaacaac gatgttatat
    14641 tttttgcaag gctatttatg gtgcggatgt cgtgttttta attgtggatg aggttat
//
LOCUS       DAZUGK010000111        14569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZUGK010000111 DAZUGK010000000
VERSION     DAZUGK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.112"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..517
                     /gene="trmL"
                     /locus_tag="ACHY5W_002955"
     CDS             44..517
                     /gene="trmL"
                     /locus_tag="ACHY5W_002955"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4447822.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(570..1391)
                     /gene="cysE"
                     /locus_tag="ACHY5W_002956"
     CDS             complement(570..1391)
                     /gene="cysE"
                     /locus_tag="ACHY5W_002956"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS4447823.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADTEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1471..2490)
                     /gene="gpsA"
                     /locus_tag="ACHY5W_002957"
     CDS             complement(1471..2490)
                     /gene="gpsA"
                     /locus_tag="ACHY5W_002957"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4447824.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2490..2957)
                     /gene="secB"
                     /locus_tag="ACHY5W_002958"
     CDS             complement(2490..2957)
                     /gene="secB"
                     /locus_tag="ACHY5W_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFS4447825.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3020..3271)
                     /gene="grxC"
                     /locus_tag="ACHY5W_002959"
     CDS             complement(3020..3271)
                     /gene="grxC"
                     /locus_tag="ACHY5W_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFS4447826.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3412..3843)
                     /locus_tag="ACHY5W_002960"
     CDS             complement(3412..3843)
                     /locus_tag="ACHY5W_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4447827.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4088..5632
                     /gene="gpmM"
                     /locus_tag="ACHY5W_002961"
                     /gene_synonym="pgmI"
     CDS             4088..5632
                     /gene="gpmM"
                     /locus_tag="ACHY5W_002961"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFS4447828.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5666..6925
                     /gene="envC"
                     /locus_tag="ACHY5W_002962"
     CDS             5666..6925
                     /gene="envC"
                     /locus_tag="ACHY5W_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFS4447829.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6929..7888
                     /locus_tag="ACHY5W_002963"
     CDS             6929..7888
                     /locus_tag="ACHY5W_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFS4447830.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFYRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(7875..8909)
                     /gene="waaH"
                     /locus_tag="ACHY5W_002964"
     CDS             complement(7875..8909)
                     /gene="waaH"
                     /locus_tag="ACHY5W_002964"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFS4447831.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKYYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(9148..10173)
                     /gene="tdh"
                     /locus_tag="ACHY5W_002965"
     CDS             complement(9148..10173)
                     /gene="tdh"
                     /locus_tag="ACHY5W_002965"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4447832.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(10183..11379)
                     /gene="kbl"
                     /locus_tag="ACHY5W_002966"
     CDS             complement(10183..11379)
                     /gene="kbl"
                     /locus_tag="ACHY5W_002966"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4447833.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVIEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            11593..12525
                     /gene="rfaD"
                     /locus_tag="ACHY5W_002967"
     CDS             11593..12525
                     /gene="rfaD"
                     /locus_tag="ACHY5W_002967"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFS4447834.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            12535..13581
                     /gene="rfaF"
                     /locus_tag="ACHY5W_002968"
     CDS             12535..13581
                     /gene="rfaF"
                     /locus_tag="ACHY5W_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFS4447835.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            13585..>14569
                     /gene="rfaC"
                     /locus_tag="ACHY5W_002969"
     CDS             13585..>14569
                     /gene="rfaC"
                     /locus_tag="ACHY5W_002969"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFS4447836.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAILGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAKGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQIVCRAPGNELSQLTANAVKRFIEKNAENAENAAM"
BASE COUNT         3641 a         3853 c         3776 g         3299 t
ORIGIN      
        1 tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact
       61 ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg ctaataccgg
      121 ctttcgtctg catatcatcg aaccgatggg atttgcctgg gacgataagc gcctgcgccg
      181 cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact acagcgcgtt
      241 cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca
      301 cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct
      361 gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc cgatggtgcc
      421 agacagccgc agcatgaacc tgtccaatgc ggtgtcggta gtggtgtatg aagcctggcg
      481 gcagctggga tatccgggag cggtgttgag agattagtta cgccatgccg gatagatgat
      541 tacatcgcat ccggcacgat cacaggacat cagatcccat ccccatactc aaatgtatgg
      601 ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc tggtttaccg
      661 acaatgcgtg ccggaacgcc agcggcggtg gtatgcggcg gcaccggttg cagcaccacg
      721 gaacctgcgc caatcttcgc gccgcgacca acttcaatat tgccgagaat cttcgcgccc
      781 gcgccaatca tcacgccttc acgaattttc gggtgacggt cgccgctagt tttcccagta
      841 ccgccaaggg tgacggattg cagaatcgac acgtcgtttt caatcaccgc cgtttcaccc
      901 acaacgatac ccgtggcgtg atcaagcatg ataccgcgac caatttttgc tgccgggtga
      961 atatcaacct ggaacgtcac agaaacctgg ttttgcagaa agattgccag tgcgcgacgc
     1021 ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa acccttcagg
     1081 tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac cgcctgaata
     1141 tcacaggcag cagaggcgat catttccgtg tcagcggcgt aggcttcttc caccacctca
     1201 cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat gtagctcagt
     1261 gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc
     1321 tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat ttccagttct
     1381 tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct gcgcgggccg
     1441 ggtcatagcg gtaacaaagg ttccctgggg ttagtggctg ctgcgctcgt ccttgcgtgc
     1501 acgacctaat aacgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata
     1561 aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt
     1621 cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt gtacatccat
     1681 gccctgaccg agcatcatgc caaaacggcg gttacgcgac tggttgtcgg tacaggtaag
     1741 caccaaatcg ccaagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc
     1801 gccaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg cattcgcacc
     1861 aaaaccgata ccgtcggaca tccccgcacc aatggcaatg acgtttttca ccgcgccgcc
     1921 aagctgcacg ccaatgaaat ccggattgct gtaaacgcgg aaacttttgc cgcagtgcag
     1981 cagctgctga agatcatcag caaaagtttg gtcggtcgag gccagcgaaa tggctgtcgg
     2041 taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg
     2101 atcgcctaac gcctcgcggg ccacgtcctg taacagacgc ccggtttccg cttccagccc
     2161 tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac
     2221 ttcaccaaag acatggctgg gtacgacgac aagtatatta cggctggctg ccagcgcagt
     2281 ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc
     2341 gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac
     2401 ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc cggcaccgat
     2461 cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg
     2521 ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc
     2581 agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac
     2641 agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg
     2701 atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccagggag
     2761 gccgttacgg taacacgcag taccacttcg taaacgtcat ctgccagttg agtagatgcc
     2821 gtatccagat caagtttaac ttctggctgc caatctttct ggaaaacgtg cggcgcgttc
     2881 ggcgcttcga aagaaatatc cttggtataa atacgttgga tctggaaagt catttcggtg
     2941 ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg
     3001 taatgccaac gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg
     3061 catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg
     3121 tgcgaccgct gcgtttgatc atctcttcac gcttggcggc gttgccatcg atcggcagct
     3181 cctggaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg
     3241 tttctttggt atagatttca acattggcca taaattagct cctgtttttt accctgaaat
     3301 ttcatgttgc agggcggcag caaaatggag tgtccccaga agcttataac aataagtcgc
     3361 tgggggccta attgcagcta acggtctgca tcatgaaaga cgacaggtaa attatttgcc
     3421 gcgcaccaaa ggcaagtttt cgccagccca gccagcgacg ccttctttca gcacgaatac
     3481 ttgcgcaaaa ccagctttcg tcagtgcgtt tgcaggctcc tggcactgca tgccagaacc
     3541 gtctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt
     3601 gatttcgctc ggcaacagat taatagaacc tgcgatatgg cctttacgga agtcatcacg
     3661 ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg
     3721 agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag
     3781 taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg
     3841 catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc
     3901 gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcgcgacgaa
     3961 aaaaaagcgt aaatctggca tcggcttgcc gcgggcggtc gtatattttg atcttcagag
     4021 gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt
     4081 tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta
     4141 tcgcgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact
     4201 gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg gtctgcctga
     4261 ccgtcagatg gggaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta
     4321 tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccggt
     4381 gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct
     4441 gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg tagaactggc
     4501 agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc gcgacactcc
     4561 gccgcgcagt gctgaatcct cgctgaaaaa gtttgaagag aaatttgctg cgctgggtaa
     4621 aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga
     4681 tcgcgttgaa aaagcttatg acctgctgac tctggcgcag ggtgagttcc aggccgatac
     4741 cgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc
     4801 gaccgttatt cgtgcagaag gtcagccaga cgcggcaatg gaagacggcg atgcgctgat
     4861 tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgctttcg tgaacgctga
     4921 tttcgatggc ttcgcgcgta agaaagtggt taacgtggat ttcgtgatgc tgaccgaata
     4981 cgccgctgac atcaaaactg cggttgctta cccaccagct tccctggtta acaccttcgg
     5041 cgagtggatg gcgaaaaacg acaaaactca gctgcgaatt tccgaaaccg aaaaatatgc
     5101 ccacgtcact ttcttcttta acggtggtgt agaagagtcg ttcaaaggcg aagaccgcat
     5161 tctgatcaac tcaccgaaag tggcaactta cgatctgcaa ccggaaatga gctccgcaga
     5221 gctgaccgaa aaactggttg cggcaatcaa gagcggcaaa tacgacacca tcatctgtaa
     5281 ctacccgaac ggtgacatgg taggtcacac cggggtgatg gaagcagcag ttaaagccgt
     5341 tgaagcgctg gatcactgcg tggatgaagt tgcgaaagcg gttgagtctg taggtggaca
     5401 actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca
     5461 ggcgcacaca gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt
     5521 gaaagcggtt gaaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg
     5581 tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc
     5641 catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat
     5701 caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc
     5761 ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg cgaaagagcg
     5821 cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga
     5881 agaagcgatc tcggaagcca cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact
     5941 gaataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc
     6001 ccaggaacgc agcctcgccg cgcaactgga tgccgcattc cgtcagggcg agcataccgg
     6061 tattcagctg attctcagcg gtgaagaaag ccagcgtgga cagcgtttac aggcttattt
     6121 cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga
     6181 agtcgccatg cagcgtgctg aactggaaga gaaacagagc gagcaacaaa cgctgttata
     6241 tgaacagcgc gcccaacagg cgaagctgac ccaggcgctg aacgagcgta aaaagacgct
     6301 ggcagggctg gagtcttcca tccagcaagg tcagcaacag ttgagcgagc tgcgcgccaa
     6361 cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag cgcgtgcaga
     6421 acgagaagca cgtgaggccc aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg
     6481 caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg
     6541 cgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc gctatggcga
     6601 gcagctacag ggtgaattac gctggaaagg gatggtgatt ggtgcctctg aaggtactga
     6661 agttaaagcg attgccgacg gtcgggtgat tctggctgac tggctgcaag gctacggtct
     6721 ggtggtggtg gttgagcatg gtaaaggcga catgagtctt tacggctata atcagagcgc
     6781 actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag
     6841 tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa
     6901 tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt
     6961 tgccgctctg ttggcgctct cctcccccgt acttgctggc aaacttgcca tcgtcattga
     7021 tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc
     7081 cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag
     7141 cggtcacgaa gtgttgattc atctgccaat ggcaccgttg agtaaacagc cgctggagaa
     7201 aaatacgctg cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt
     7261 caataacgtg ccctatgccg tggggatcaa caaccacatg ggtagcaaga tgacctctaa
     7321 cctgtttggt atgcagaaag tgatgcaggc actggagcgt tacaatcttt acttcctcga
     7381 cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt
     7441 tattaaacgg aaggtgttcc tcgacgattc acagaacgaa gcagacatcc gtgtgcagtt
     7501 ttatcgtgca attgacctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca
     7561 cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct
     7621 ggtaaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac cgccgaaaaa
     7681 cactgtgccc gacacaccgc gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa
     7741 accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca
     7801 gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc
     7861 ggcgaaactt aacgctagcg caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc
     7921 gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctggta acccaccttc
     7981 acgctgcgca cattggtaat caggcgctta tacataccgg aagtaaatat ctcggcaatc
     8041 atccgttggc gggtaagaat atccggctct ttgcgcaccg catggcaaac acgcaatgct
     8101 tcgtaagtta tttgctgatg aaattccgga taaatcgtaa ttttgtcggc ataatttcga
     8161 tttaatttct ccagcaggcg ggtaatctta atatagtgac gttgataatt aaggttttta
     8221 ttcccttgtc tatgcaaccg actcactgac gtattatgca gataatattt atataatgat
     8281 tgctcggtat atcgcgcacg cagcgcgtta aacatgaatt ctgttgtcca gacaatatcc
     8341 tgatgatgta atccggcaat aaatttaatg ttatttttaa caataacatc gcggcgataa
     8401 acccccatcc agaccacgtg tgtccaacgg cgcgaagaaa gccccatccg cagccagtcc
     8461 gggccggtta agacgccggt tgagcgaagg cgatcggtgg ggatggattg ccaggtttct
     8521 cccgtttcac gaaaacacca gtcagcgttg cactgcgcca cgtcgaggtc gtcctctaac
     8581 gccatggtca tcagcgtttc gtacatggtg ggatagactt catcgtcagc atcgacaaaa
     8641 gcgacatatt tgcccgttgc cacttcaatc ccacgattac gcgccaccga tgcgccagca
     8701 ttcgcctgat gcaacaaacg aacgtgcgga tagttttctg cgtaatactt tgctatttca
     8761 acagaattat ccgttgaacc atcgttaata ataatgattt ccagagcagt ccaggtttgc
     8821 gtaattaaag attccataca agtacggaaa tcatcgcccg cattatataa cggaataata
     8881 acactaagtt tattggtgct gttcatcata aattaccgac gacctctgaa gttatcctga
     8941 cgttttacag gctttgtatt aagaaactat taagcctgtg agatattatt tttacaggtg
     9001 agagagtcac ttcagggtag agagataatg agagcagcta tataagagat cagcacaatt
     9061 acgaactctt tattacactg acaggatttt acatatttaa cagattatcc tcagataaaa
     9121 gggctggaat accagccctt gttcgtgtta atcccagctc agaataactt tcccggactg
     9181 gcccgaacgc atagcgtcaa agcccttctg gaaatcatcg atagagaaac gatgggtaat
     9241 gatcggcgag agatcgaggc cagactgaat cagcgccgcc atcttgtacc aggtttcaaa
     9301 catctcacga ccgtaaatac ctttaatgaa caagccttta aagatcactt tggtccagtc
     9361 gatagacata tcagacggtg gaatacccag catcgcaatg cgaccgccgt ggttcatggt
     9421 gtcgagcatg gtacgaaacg ctggcggcgc accagacatt tccagaccga catcaaagcc
     9481 ttcggtcatg cctaactccg ccatcacgtc attgagattt tctttggcga cgttaaccgc
     9541 acgggtgata cccattttac gcgccagctc aaggcggtat tcattaacat ccgtgatcac
     9601 cacgttgcgt gcaccaacgt gtttcgccac cgccgcagcc ataataccta tcggccctgc
     9661 accagagacc agcacatcct cgccaaccag atcgaacgac agcgcggtat gcacggcgtt
     9721 accgaaggga tcaaaaatgg aagccaggtc gtcggagata ttgtctggga ttttgaaggc
     9781 gttgaacgcc gggatcacca gatattcggc gaagcagccc ggacggttaa cgccgacgcc
     9841 gatcgtgttg cggcacagat gggtacgacc gccacgacag ttgcgacagt gaccgcaggt
     9901 gatatggcct tcgccagaaa cgcgatcgcc gattttgaag cctttcactt cctgaccaat
     9961 acctaccact tcaccgacat attcatggcc tacgaccatc ggcaccggga tggttttttg
    10021 cgaccactca tcccagttat agatgtgaac gtcagtcccg cagatggctg ttttacggat
    10081 tttaatcagc agatcgttat gcccgagttc cggtacagga acgtcggtca tccagatgcc
    10141 ctcttccgct ttcagtttgg ataacgcttt catctcacat cctcaggcaa taacgcccag
    10201 ttgtttacca atacgcgtga atgcttctac tgcacgcgta atttgctcag gggtatgcgc
    10261 cgcagacatc tgggtacgaa tacgcgcctg acctttcgga acgaccggat agaagaaacc
    10321 ggtgacgtaa atgccctctt tttgcagctc acgggcaaat ttctgcgcca ctactgcatc
    10381 gccaagcatg accggaataa tggcatgatc ggctcccgcc agggtaaagc ccgccgccga
    10441 catttgctca cggaactgac gcgcgttcgc ccacagacgg tcacgcagct cgctacccgc
    10501 ttcaaccatc tccagcactt tgatagaagc ggcaacaatg gccggagcca gcgagttgga
    10561 gaacaggtac ggacgagaac gctggcgcag ccactcaatc acttctttgc gcgccgcggt
    10621 ataaccacca gaagccccgc ccagcgcttt accaagcgta ccggtgataa tatcgacccg
    10681 gcccatcaca tcgcagtatt catgggaacc acgaccattt tcaccgacaa aaccgaccgc
    10741 gtgggagtcg tctaccatca ccagggcatc gtatttatct gccagatcgc aaacgccttt
    10801 caggttggca atcacgccgt ccatcgagaa cacaccatcg gtggcaatca gcacatgacg
    10861 cgcaccggct tcacgcgctt ctttcagacg tgcttccagc tcctgcatat cgttgttggc
    10921 atagcgatag cgtttagctt tacacagacg cacaccatca ataatagacg cgtggttcag
    10981 tgcgtcggag ataatggcgt cttccgcgcc cagcagcgtt tcaaacaggc caccgttagc
    11041 atcaaagcag gaagagtaga gaatcgcatc ttccatcccc aggaatgccg ccagtttttg
    11101 ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa cgcaccgaag ccatgccgaa
    11161 accatgagaa tccattcccg cctttgccgc cgcaatcaga tccggatgat tcgccagccc
    11221 gagatagtta ttggcacaaa agttaatgac gtggcttcca tcagccacag tgatatctgc
    11281 ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac ccttccgccc gtgcggtttc
    11341 cagatcgttg gttaactgct gataaaattc tccacgcatt gcgattctcc agacagggca
    11401 aattccagca catattaccc aaacttatag gtcgcgacga gatgacgcgt taacacttct
    11461 gcaaaattca ggataaataa cgtccatcaa tctcactttt ggcgaatggc tgaagccaac
    11521 gccgtgtagt gatatgatag aagtattcgt gtctgagatt gtctctgact ccataattcg
    11581 aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag caacatcgtt
    11641 aaggccctga atgataaagg catcaccgat attctggtgg tggacaacct gaaagacggc
    11701 accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa ggaagacttc
    11761 ctgatccaga ttatggctgg cgaagagttc ggcgatgtcg aagcgatttt ccacgaaggc
    11821 gcatgctctt ccaccaccga gtgggatggc aagtatatga tggataacaa ctatcaatac
    11881 tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta tgcatcttcc
    11941 gcggccacct acggcggacg cacctccgac tttattgaat cccgcgagta cgaaaaaccg
    12001 ttgaacgtct acggttactc aaaattcctg tttgatgaat atgttcgtca aatcctgcct
    12061 gaagcgaact cgcagattgt tggcttccgt tatttcaacg tttatggacc gcgtgaaggc
    12121 cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa caacggtgaa
    12181 tcgccaaaac tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta tgtgggcgac
    12241 gtggcagatg taaacctgtg gttcttggaa aatggcgttt ccggcatctt caacctcggc
    12301 accggtcgtg cggaatcctt ccaggctgta gctgatgcta cgctggctta tcacaagaaa
    12361 ggccagatcg aatacattcc gttcccggat aagctgaaag gccgctacca ggcgttcact
    12421 caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa aaccgttgct
    12481 gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct ctgcatgaaa
    12541 atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag tctctatcgc
    12601 acgctccagg cgcgctatcc ccaggcgata atcgatgtga tggcaccggc atggtgccgt
    12661 ccattattat cgcggatgcc ggaagttaac gaagctattc ctatgcctct cggtcacgga
    12721 gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa gcgctacgac
    12781 cgcgcctacg tcttacccaa ctccttcaaa tctgcattag tgcctttctt cgcgggtatt
    12841 cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga tgtacgcgtg
    12901 ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc ctatgacaaa
    12961 ggcgtgatgc gtaccgcaca agatctacca aaaccgttgt tatggccgca attgcaggtg
    13021 agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga acgtccgatg
    13081 attggttttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca ctaccactat
    13141 gcggaactgg caaagcagct gattgatgaa ggttatcagg tggttctgtt tggctctgcg
    13201 aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca gcaggcatgg
    13261 tgtcggaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt
    13321 aaagccattg tcactaacga ttctggcctg atgcatgttg cggcggcgct caatcgtccg
    13381 ctggttgccc tgtatggtcc gagtagcccg gacttcacac cgccgctatc ccataaagcg
    13441 cgcgtgatcc gcctgattac cggctatcac aaagtgcgta aaggtgacgc tgcggagggt
    13501 tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact caacgcgcta
    13561 ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc gtcgatgggc
    13621 gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaattct agggattaaa
    13681 tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc tgccgttgag
    13741 cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc ccccataaaa
    13801 gcagaacgca aagcgtttcg tgaagcgcta caagcagaga actatgacgc agttatcgac
    13861 gctcaggggc tggtaaaaag cgcggcgctg gtgacgcgtc tggcgcatgg cgtaaagcat
    13921 ggaatggact ggcaaaccgc tcgcgaacct ttagccagtc tgttttacaa tcgtaagcat
    13981 catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc caaaagtctg
    14041 ggctatagca aaccgcaaac ccagggcgat tatgctattg cacagcattt cctgacgaac
    14101 ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg tgatgataaa
    14161 cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc aggaatacgg
    14221 attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact ggcgaaagga
    14281 tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg cgtgctggcc
    14341 ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc ggcactggat
    14401 agacccaata tcacggttta tggaccaacc gatccgggat taattggcgg atatgggaag
    14461 aatcagatag tttgtagagc tccggggaat gagttgtctc aattgacagc aaatgctgtt
    14521 aagcggttca ttgaaaaaaa cgctgaaaac gctgaaaacg ctgctatga
//
LOCUS       DAZUGK010000112        14374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZUGK010000112 DAZUGK010000000
VERSION     DAZUGK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.113"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHY5W_002970"
     CDS             complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHY5W_002970"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFS4447837.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKYTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGSFSKEDGEKVVDEAGNRLK"
     gene            1464..1547
                     /gene="dinQ"
                     /locus_tag="ACHY5W_002971"
     CDS             1464..1547
                     /gene="dinQ"
                     /locus_tag="ACHY5W_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFS4447838.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1601..2953)
                     /gene="gorA"
                     /locus_tag="ACHY5W_002972"
     CDS             complement(1601..2953)
                     /gene="gorA"
                     /locus_tag="ACHY5W_002972"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4447839.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTELQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(3025..3867)
                     /gene="rlmJ"
                     /locus_tag="ACHY5W_002973"
     CDS             complement(3025..3867)
                     /gene="rlmJ"
                     /locus_tag="ACHY5W_002973"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFS4447840.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            4070..6112
                     /gene="prlC"
                     /locus_tag="ACHY5W_002974"
     CDS             4070..6112
                     /gene="prlC"
                     /locus_tag="ACHY5W_002974"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFS4447841.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRSFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGISAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            6120..6872
                     /gene="rsmJ"
                     /locus_tag="ACHY5W_002975"
     CDS             6120..6872
                     /gene="rsmJ"
                     /locus_tag="ACHY5W_002975"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFS4447842.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6921..8390)
                     /gene="dtpB"
                     /locus_tag="ACHY5W_002976"
     CDS             complement(6921..8390)
                     /gene="dtpB"
                     /locus_tag="ACHY5W_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFS4447843.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIATLVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8556..8609
                     /locus_tag="ACHY5W_002977"
     CDS             8556..8609
                     /locus_tag="ACHY5W_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447844.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8587..8640
                     /locus_tag="ACHY5W_002978"
     CDS             8587..8640
                     /locus_tag="ACHY5W_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447845.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8707..9141)
                     /gene="uspA"
                     /locus_tag="ACHY5W_002979"
     CDS             complement(8707..9141)
                     /gene="uspA"
                     /locus_tag="ACHY5W_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFS4447846.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9532..9867
                     /gene="uspB"
                     /locus_tag="ACHY5W_002980"
     CDS             9532..9867
                     /gene="uspB"
                     /locus_tag="ACHY5W_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFS4447847.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9938..11437)
                     /gene="pitA"
                     /locus_tag="ACHY5W_002981"
     CDS             complement(9938..11437)
                     /gene="pitA"
                     /locus_tag="ACHY5W_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFS4447848.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11669..12871
                     /gene="yhiN"
                     /locus_tag="ACHY5W_002982"
     CDS             11669..12871
                     /gene="yhiN"
                     /locus_tag="ACHY5W_002982"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4447849.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAVRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDADLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISALTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(13186..14238)
                     /gene="yhiM"
                     /locus_tag="ACHY5W_002983"
     CDS             complement(13186..14238)
                     /gene="yhiM"
                     /locus_tag="ACHY5W_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFS4447850.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSEQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWTLLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3654 a         3635 c         3759 g         3326 t
ORIGIN      
        1 atccagtacg tctttaaact gctgcactgt atcgcggata actaagcagc tcactgatac
       61 accatgctgg acgcgataat tggaatctca cgcagggagt aatgtgtaaa tagcaaaatc
      121 gcctgatgcg ctacgcctgt caggcctaca aataacatac tattcatcga attttcataa
      181 ctttgtaggt cggataagac gcgccagcgt cgcatccgac ataaataaag cgcacttttc
      241 taacaacctg tgggggggat atagccgctt tatttcaggc gattacccgc ttcatcaacc
      301 actttctcgc catcttcctt ggagaatgag cctttttgcg catctggcag aatttccagc
      361 acgacttctg aagggcggca cagacgagtt cccagcgtcg tcaccacaat cgggcgatta
      421 atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtata tttatcttcc
      481 gcaaggccca gctcctcata cggctcgacg tttttacgca gcagcgcgcg tacggaaatc
      541 cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg
      601 ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg
      661 caggccgggt tgtgataaat ggtaatgttg ctcatatcat tatctcatta caaagtgaaa
      721 gagagacgta gcgccagcgc caccagcgtt acaaaccaaa gcggataagt caaaagcata
      781 tatggtttaa cgaatgcgta tgtgcagagg aaggtaatag gtgtgaattt tgagttggct
      841 attcatttga aaggaggtct gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt
      901 actttccagc cctgagttgg tggctctggc cggagtgaga atgcccacat ctaacggttc
      961 aatactatta gccagtggct aacattgcaa gcgttagcga tttcttcagc tggagaaggg
     1021 acagtttgct atacatgttg gtgaaagagg atttatatac tttcatcggg tcgctgccgt
     1081 accaagcctt gtccgaatcc ggacgtaaaa aaaccgactt cgcgtcggtt tttttactct
     1141 tcagcccaga gttggtggct ctggctggag tgagaatgcc atcatctaac gcatcaatgc
     1201 taataccata ctgaaactat tgcaaggaca tgctggtttt ataacctgaa tatactgtat
     1261 gattatccag ttagctctga ggcattttca ctctggcaat gcgcataaac gctttcaaag
     1321 tcctgatcag tggtacgggt ggtgccgtta actgatgctc tggccggagt gagaatgcca
     1381 ttatctaaca atagggcatg cgccgtgaca ggcaatggat gagtaagcgg atgcattctc
     1441 actccatcgt atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa
     1501 ttgcgctgtt ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta
     1561 attaagggct aagagcacac tactcttagc cctttaacat ttaacgcatg gtcacgaact
     1621 cttctgccgc cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgccccca
     1681 tcttcagcgc caccgcgaag ccctgcaaca tttcgtccat accaaaacca atgccgtgaa
     1741 taccgacaat cttctcttcc ggcccaacgc acaccagctt catgcggcac ggctggcggt
     1801 gagtggtgac ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt
     1861 cgccgtactg ctcgcgcgcc tgcagttccg ttaaaccaac agtaccaatc ggcggatggc
     1921 tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc atccggtttg ttattaaaca
     1981 ggcgttcaga gagacggcga cccgctgcaa ctgccaccgg tgtcagttct actgcacccg
     2041 tgttatcgcc caccgcgtaa ataccttcaa tgttggtgtt ttgatattta tcgacgacga
     2101 tatagccttt ttcgttggtt ttaacgccag cggcttccag gttgatgttg tcattggcag
     2161 gctcgcgacc aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct
     2221 ccagcgtcag gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca
     2281 actgcgggcc ttcggcgttc atcacttcga ccagcgtttc agagatcatc gggtcgaagc
     2341 tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca
     2401 cgccagccag ctcaacggcg atgtaacccg cgccaacaac cgccacacgc tctggcaaag
     2461 caggaagggc aaagaaacca tcagaatcaa taccgtattc cacgcccgga atatccgggt
     2521 ggctcggacg accgccggtg gcgatcagaa tatgatcggc tgtgatggtt tcgccattaa
     2581 cttccagcgt tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt
     2641 taccgagcac gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga
     2701 tcaacgtttc ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca
     2761 tatggatcgc ttcacggatt tgcgccgcgt gccacatcac tttcttcggt acgcagccga
     2821 cgttcacgca ggtgccgccc agctctttgg cttcaatcag cgcgcatttc tgaccgtaca
     2881 tagccgcgcg gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat
     2941 agtgtttagt catgacagag tgtccttatc gttgattacc gcgattgtag cgcgagacgt
     3001 aataggtgcc agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc
     3061 ggtgcctgcc ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgttgttc
     3121 cagcttccac ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct
     3181 gtctggcagt accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc
     3241 gtggatcatg cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc
     3301 agtggcgaaa cgtttgtaac cttctgctat cccgctgacc accgcttgat agtcggtttt
     3361 catttcatac ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc
     3421 cttaagctgc tggaaaccgt cggctttttc aacgcgcgca cggctatctt tctgaaattc
     3481 agaacgcagc aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcttgttc
     3541 gcgcagtagt tggcgagcaa tcaacggcga accggggtag taacgcaact gaccgctacg
     3601 gttgaagtgt tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg
     3661 ccagatacgg gcgatacctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa
     3721 ctgataacgc ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt
     3781 cagcgactcg atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc
     3841 gtgaaagctg tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc
     3901 cgtattcggg gcgcacaaaa gcctatcagg acagtatacc gaaaagaggc cgccgccgcg
     3961 aaagcgtaac gtttctcatt gaaattcact acacttaacc ccatgctaca aacattatgt
     4021 aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg accgcgctaa tgacgaatcc
     4081 gttactgact ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc
     4141 agccgtgact caggcgctga acgactgccg ggaaaatgtg gagcgcgtag tagcgcaagg
     4201 ggcaccgtac acctgggaaa atctctgcca gccgctggcg gaagtggacg atgtgttggg
     4261 gcgtatcttc tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga
     4321 agcctacgag caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga
     4381 agggctgtat aaagcgtatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac
     4441 ggcgcagaag aaagcggttg ataacgcact gcgcgacttt gaactctctg gcatcggtct
     4501 gccgaaagag aaacagcagc gttacggcga aattgctacc cgtctttctg aactgggcaa
     4561 tcagtacagc aacaacgttc tcgatgcgac gatgggctgg accaaactcg ttaccgacga
     4621 agcggagctg gcggggatgc cagaaagcgc gctggctgcg gcaaaagccc aggccgaagc
     4681 gaaagagctg gaaggttatt tgctgacgct ggatatccca agctacctgc cggtaatgac
     4741 ctactgcgac aaccaggctc tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc
     4801 cgatcaaggc ccgaacgccg gtaagtggga caacagcaag gtgatggaag agatcctcgc
     4861 gctgcgtcac gaactggcgc aactgctggg ctttgaaaac tacgccttta aatcccttgc
     4921 cactaaaatg gcagaaaatc cgcagcaggt gcttgatttc ttaaccgatc tggcaaaacg
     4981 cgcacgtccg caaggcgaaa aagagctggc acaactgcgt tccttcgcca aagccgaatt
     5041 tggcgtcgat gagttgcagc cgtgggatat cgcttactac agcgaaaaac aaaaacagca
     5101 cctctacagc atcagtgacg aacagctgcg tccgtacttc ccggaaaaca aagcggttaa
     5161 cggcctgttt gaagtggtga aacgtattta cggcatcagc gctaaagagc gtaaagatgt
     5221 tgatgtctgg catccggatg tacgtttctt cgaactgtat gacgagaaca acgaactgcg
     5281 aggcagcttc tacctcgacc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga
     5341 tgactgcgta ggccagatgc gtaaagccga cggttctctg caaaaaccgg ttgcttattt
     5401 gacctgtaac tttaaccgcc cggtaaatgg taaaccggcg ctatttaccc atgacgaagt
     5461 gatcaccctg ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcattgaaac
     5521 cgccggtgtt tccgggatca gcggggtgcc gtgggatgcg gtcgaattgc cgagccagtt
     5581 tatggaaaac tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac
     5641 cggcgaaccg ctgccgaaag agttgctgga taaaatgctg gcggctaaga actaccaggc
     5701 ggcgctgttt attctgcgtc agctggagtt cggcctgttt gatttccgcc tccatgccga
     5761 gttccgcccg gatcaggggg caaaaatcct cgaaactctg gcagaaatca agaaactggt
     5821 tgccgtagta ccgtctccat cctggggccg tttcccgcac gctttcagcc atattttcgc
     5881 cggtggttat gccgcaggtt actacagcta cctgtgggcc gacgtgctgg cggcagatgc
     5941 cttctcgcgc tttgaggaag agggcatttt caaccgtgaa accggacagt cgttcctcga
     6001 caacattctg agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg
     6061 tcgtgaaccg cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag
     6121 tgaaaatctg cttaattgat gaaacaggca ccggagacgg tgccttatct gttctggcgg
     6181 cccgctgggg gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac
     6241 atctggaatt gcgcaaacgt gatgagccaa aacttggcgg catctttgtt gattttgttg
     6301 gcggagcgat ggcgcaccga cgcaaattcg gcggtggtcg cggtgaggcg gtggcgaaag
     6361 cggtgggcat taaaggcgat tatttgccgg atgtggtgga tgccaccgca gggctggggc
     6421 gcgatgcctt tgtgctggct tcagtcggct gccgcgtgcg gatgctggag cgtaatccag
     6481 tcgttgccgc attgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg
     6541 gctggttgca ggagcggttg cagttaattc acgcctccag cctgacggcg ctaaccgata
     6601 ttaccccacg cccgcaggtg gtctatctcg atccgatgtt cccgcataag cagaaaagcg
     6661 cgctggtgaa aaaagagatg cgtgtctttc agtcgctggt ggggccggat ctcgacgcgg
     6721 atgggttgct ggagcctgcg cggctgctgg cgactaaacg cgtggtggta aagcgcccgg
     6781 actacgcgcc accgctagcg aatgtcgcca cgccaaacgc ggttgtgact aaagggcatc
     6841 gctttgatat ctatgcaggt acgccagtgt aaaagtaaag cagggtagcg ataacgctac
     6901 cctgctttct gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac
     6961 ggcaccatca acagcatcac tactgcaacg cccagcgtga ccagaccaat cttaccaaac
     7021 acgttggtat agacgggcaa cgtctcaagc ggatcggtaa tgttgtccgg caccgcggta
     7081 aatgttgcca catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg
     7141 agaataaaac ccatcaaatg ctgcggcacc agtgtagcaa tcatcgccag gccaagggcg
     7201 ctaataaaca gttcacccag gctctggaat aagtacacca gcacgataaa ccatggcgat
     7261 gtcagccctt gtgcatcagc aaaccacatt cccgcagctg ccgccgtcaa aaagcccagt
     7321 gagcacataa acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta
     7381 cccagatgcg tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta
     7441 agcgcctgga agctgaccgg gttgatggaa aagccgagaa tttcatgatg cacgttgttg
     7501 atggcaaaga agttcagcga cgttggcatc tgggcgtaga gaatgtaaaa caccaccgct
     7561 tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat
     7621 gcctgacgaa agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg
     7681 acttctacgt tgtgcatcag ccatgcacag acgaagatca tcaccacgct gccaagtaac
     7741 acgtacaaca gtttgctgaa gctcatcggc cggaagtcgg gttcagaacc aatgtctttc
     7801 accattccac gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg
     7861 ttgtaggtga cagaataacc gaatctatca gcgatcacag gggccagcga taacgctatc
     7921 aacgagccga tgttgatcga catatagaac agggtgaatg cgccatcaag gcgcggatct
     7981 ttcggcggat agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg
     8041 acagcgatag tccccagggc gatgaaaatc aggtcaggct taagtagcga caggccggtc
     8101 atgaagtagc caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc
     8161 aggtggtcgc cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca
     8221 ccaaaagtga caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc
     8281 agtacgccct gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg
     8341 aaaaatgggc gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac
     8401 cttccaatac catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca
     8461 tctgtcagag attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa
     8521 ataaccagct gatcacacta atgatggaaa atatgatggt gttaaatatt gtattggctg
     8581 gggattttga ttgcgaacgc agaccgtagg ccagataagg cgtttacgct gatcaggtaa
     8641 caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtggag
     8701 ggaagattat tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt
     8761 gatcagctga cgtgcggaag acatcagttt gctccagaag tcctggtggt gaccacaaac
     8821 caccagatcc atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct
     8881 gccgctcagg gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg
     8941 atgtgtctct tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt
     9001 gtataggtca gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg
     9061 agccatagag actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat
     9121 gagaatgtgt ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag
     9181 ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac
     9241 ccagccgtaa agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt
     9301 caatccgcct tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg
     9361 ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg
     9421 ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg
     9481 gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc
     9541 accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc
     9601 tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat
     9661 gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt
     9721 tggtatatct atgcccaacg ttatcgcgat catcacgatg atgagtttat tcgccgctgt
     9781 gagcgggtgc gtcggcagtt tattctgact agcgcattgt gtggtctggt ggtggtcagc
     9841 ctgattgcat tgatgatttg gcattaagct cttcagcaat aataacgcat aaaaaaagcg
     9901 ggccagtaaa ctgacccgct cgttttggtg tgtacgatta caggaactgc aaggagagcc
     9961 agtacagccc gccggaaagc agtaccgcag ccggaagggt aaacacccag gccatcagaa
    10021 tgctggtcac ggttttacgc tgtaagccgc caccatctac caccatcgtc cccgcgacag
    10081 aagaggagag tacgtgagta gtggaaaccg gcatcccggt ataactcgcc aggccgatag
    10141 acactgccgc cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct ttcttaccga
    10201 ttttctcacc gatagtcgtt gccacacgac gccagccaat catcgtaccg atacctaacg
    10261 ccagcgcgac cgccatgatg atccacaccg gcgcatactc gatggtgcta agcatgtcgg
    10321 acttcagttt cttcaacagg cgctgatcgt cagcactcac gccaggcatc ttcaccactt
    10381 tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt tgatcaagcg
    10441 acagcttgtc gtagctttcc acatcggtag tcaacatgcc tttcaggcgg ttgagcgcgt
    10501 taatggtatt cgacggatgg caatggaact ccgcgggttg cgttgcgcca gcttccggag
    10561 ccggtactaa ttgatcagca ccagtagctt gtttcagcag cgcaggatgc tgctcaaagt
    10621 aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca gtggcattca
    10681 tgttcaccac gaagcctgct ggtgcgacgc caatcaatac caacataacc agaccaatgc
    10741 ctttctgacc atcgtttgcg ccgtgcgaaa acgccacgcc gatagcggaa aggatcagcg
    10801 caatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc tccgctgggg
    10861 tcaggtggat tcgggcgcgt ttcttggtgc cgctccagta gcgacgcagc aagaaaatca
    10921 gaccgccagc aaacaccaga ccgacaatag gggaaacgat cagagaaccg aaaatactta
    10981 atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac gcattggtta
    11041 aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt aaaccaaagt
    11101 accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc atggcaaggc
    11161 catgagacga tcccatatta agcagcagat ctgtcggcag catatgcaca atggcatagg
    11221 caacactcag accacccagc aaaacaccca aaaagttgaa caccgctgcc ataaccacgg
    11281 cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg gctgtgtcat
    11341 ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat aataacagcc
    11401 cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc attttgagga
    11461 catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa tatagacttt
    11521 ttttgatttg cctcctgtat ggatttcact caaaaaataa ttatcttata taattcaggc
    11581 aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct ttacaattca
    11641 cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt gatgccatta ttataggcgc
    11701 tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc gggttctgct
    11761 gatcgataat ggtaaaaaac cagggcgcaa aatccttatg tctggcggtg ggcgctgcaa
    11821 ctttaccaac ctttatgtcg aaccgggcgc ttatctgagc cagaatccgc atttttgtaa
    11881 gtctgcactc gcgcgtttta cccagtggga tttcattgat ctggtcaata aacacggcat
    11941 cgcctggcac gagaaaacgt tagggcaact cttctgcgat gactccgctc agcagattgt
    12001 cgacatgctg gtggatgagt gcgagaaggg caatgtggcc gtcagattgc gtagcgaagt
    12061 gctgagcgtg gagaaggatg atacaggttt cacgcttgaa ctgaacggca tgactgtcgg
    12121 ttgcgaaaag ctggtcatcg cgaccggtgg gctgtcaatg ccggggctgg gcgcatcgcc
    12181 gtttggttat aagattgccg aacaatttgg cctcaacgtg ctgccgaccc gcgcgggtct
    12241 ggtgccattc actctgcata aaccgttgct cgaagagtta caggtgctgg cgggcgtggc
    12301 ggtgccttcc gtgattaccg ctgaaaacgg caccgttttc cgtgagaact tactcttcac
    12361 ccatcgcggc ttgtctggac cggcggtgtt gcaaatttcc agctactggc aaccggggga
    12421 atttgtcagt atcaatctgc taccggatgc ggacctcgaa accttcctga atgagcagcg
    12481 taacgcacat ccgaatcaaa gcctgaaaaa cacactggcg gttcatctac cgaagcggtt
    12541 ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt tcgctaaaac agctcaacgt
    12601 gcgtgaccaa caggcactga ttagcgcatt gaccgactgg cgcgtacaac ccaacggcac
    12661 tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg aactctcttc
    12721 acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag tgatggacgt
    12781 caccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcat gggcttgtgc
    12841 gcaggatttg attgcagcga agtcgtcctg acctattatc taaagatgaa aattattaga
    12901 gccgcattaa aaatgagcta attttgatag tggttatctt gtgattattt tctaatgagc
    12961 ccgtgagctg aaaccctcca tgcttaataa aaggtggagg gaaagtgatt gaaaactcac
    13021 ccagtgatca gctctttata aaacatgaac aggttgaaaa agacagtaac tttgctgttt
    13081 ttttatcctt aaccactatg cattaatgct gcgttatttc attctaccta agaaaaacca
    13141 gcgttacgca aatggtcaac gctggtttta tccggtacgt tgcaattatt ttttagcaga
    13201 acccgcttct aatattgaaa cgattgagaa caacgtaaag cataccgctc ccaaaccgac
    13261 cagaacccgc gaaggaataa aatatcccat gtcggtctgc gccatttccg caagaaacga
    13321 tgccagaaaa aggcaaaaca gacaggtaaa taccggtatc atcggtattc tgttggctaa
    13381 cgaacaggta cgtctccaga ccagtgccag tagccagact tttgagaata tgctgtaaca
    13441 gatcattccc aagcaaatga ggataatacc gggagccagt cgggcgcttg catcagaact
    13501 gactaacacg tatatgccct gcagcaccgt gattgagccg agaaaaataa cccaataaca
    13561 ccacagccag tgctctttgg aagagaacgt attacgtgtt tgatggacga ttgtggcaac
    13621 aaggccaatt agacaggcac agattgcggt taacccgagc aatacgtgac cggcgacata
    13681 atgcggagtt atgtcggcac tacgtaacag cgtaatagac caaatgaaac cgagcagggt
    13741 gagtaacacg ggaacggcaa tgaggcaatt accgattaat gaagaataag cctgtaccgg
    13801 tgttccgtcg ctcttgctcc ccgctgcgtt tttaggaatg agcagaaagt gaccggatga
    13861 cgctgccact gtcgatacac aggcggcaat cataccaacg ccgaaaataa catggccggc
    13921 gacaaactcg tctgccatca catcattacc tgctaacagt gtccaacccc atatcatggt
    13981 gataattgac cccgcatagc caataatggg gaacaatgta ttgtaaaacg tattaacgcc
    14041 gcgcgtgagc tgcgaaatga taataaatgc ggtagtgaat aatgccaggc atatggctgc
    14101 cagagaaatc agcacatgac ccgcgacgaa atactcgctt tgctctgaac tttccagcac
    14161 aaagccacct aacgcgatac aaatcaagcc cataattaaa gggattaatt tgaaaagcca
    14221 cccgatatat atgttcactt taaactcctc aggtatccct gctaatattg aaatgtgcat
    14281 taatgataat ttcagcctgt taatacaatg acgagatatc attaaagaca aatgcatagc
    14341 ataactatag cggggtttac tttgcagagc gcta
//
LOCUS       DAZUGK010000113        13996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZUGK010000113 DAZUGK010000000
VERSION     DAZUGK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.114"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..438
                     /locus_tag="ACHY5W_002984"
     CDS             <1..438
                     /locus_tag="ACHY5W_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4447851.1"
                     /translation="SLRTWIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAIL
                     DGVLINYIAICLASARKKQGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLN
                     QLRPELFEGRTLLVWCGENRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            498..605
                     /gene="yohO"
                     /locus_tag="ACHY5W_002985"
     CDS             498..605
                     /gene="yohO"
                     /locus_tag="ACHY5W_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFS4447852.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(586..1317)
                     /gene="yehW"
                     /locus_tag="ACHY5W_002986"
     CDS             complement(586..1317)
                     /gene="yehW"
                     /locus_tag="ACHY5W_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFS4447853.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1322..2248)
                     /gene="yehX"
                     /locus_tag="ACHY5W_002987"
     CDS             complement(1322..2248)
                     /gene="yehX"
                     /locus_tag="ACHY5W_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFS4447854.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            complement(2241..3398)
                     /gene="yehY"
                     /locus_tag="ACHY5W_002988"
     CDS             complement(2241..3398)
                     /gene="yehY"
                     /locus_tag="ACHY5W_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFS4447855.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3405..4322)
                     /gene="osmF"
                     /locus_tag="ACHY5W_002989"
     CDS             complement(3405..4322)
                     /gene="osmF"
                     /locus_tag="ACHY5W_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFS4447856.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLREYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(4533..6830)
                     /gene="bglX"
                     /locus_tag="ACHY5W_002990"
     CDS             complement(4533..6830)
                     /gene="bglX"
                     /locus_tag="ACHY5W_002990"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFS4447857.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIVALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7026..8741
                     /gene="dld"
                     /locus_tag="ACHY5W_002991"
     CDS             7026..8741
                     /gene="dld"
                     /locus_tag="ACHY5W_002991"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS4447858.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWLDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8779..9711)
                     /gene="pbpG"
                     /locus_tag="ACHY5W_002992"
     CDS             complement(8779..9711)
                     /gene="pbpG"
                     /locus_tag="ACHY5W_002992"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFS4447859.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9885..10472)
                     /gene="yohC"
                     /locus_tag="ACHY5W_002993"
     CDS             complement(9885..10472)
                     /gene="yohC"
                     /locus_tag="ACHY5W_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFS4447860.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            10642..11220
                     /gene="yohD"
                     /locus_tag="ACHY5W_002994"
     CDS             10642..11220
                     /gene="yohD"
                     /locus_tag="ACHY5W_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS4447861.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11350..12111)
                     /gene="yohF"
                     /locus_tag="ACHY5W_002995"
     CDS             complement(11350..12111)
                     /gene="yohF"
                     /locus_tag="ACHY5W_002995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4447862.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12164..13600)
                     /gene="mdtQ"
                     /locus_tag="ACHY5W_002996"
     CDS             complement(12164..13600)
                     /gene="mdtQ"
                     /locus_tag="ACHY5W_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFS4447863.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            13824..13907
                     /gene="yohP"
                     /locus_tag="ACHY5W_002997"
     CDS             13824..13907
                     /gene="yohP"
                     /locus_tag="ACHY5W_002997"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFS4447864.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3314 a         3911 c         3536 g         3235 t
ORIGIN      
        1 agcctgcgaa cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca
       61 catctgttta ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg
      121 ctggcgatcc tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt
      181 aaaaaacagg gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg
      241 tggctggagg gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg
      301 ctcaatcaac tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa
      361 aatcgaacct ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt
      421 ttcccactcg gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt
      481 aacataagga gtgcattatg cgcatagcta aaattggggt catcgccctg ttcctgttta
      541 tggcgttagg cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg
      601 gctaagcgcc tgcaccagcc tttcaaacag gcggtctgcg atgatcgccg ccagtgccac
      661 cagtaacgcc ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg
      721 cgtacccagc gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat
      781 caccgaagtt cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg
      841 cagtcgctga ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat
      901 cgctcccagc ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc
      961 gataatcgct ggttgcagac caaagccgat caccggaacg gcgatcgcca gaactgcgac
     1021 gggcggaaaa gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc
     1081 ccacgggcga gtgacagcaa ttccggcacc agtgccaatg atcaccgcaa acaaactcga
     1141 aattcccacc agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac
     1201 gggtcgtggc agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa
     1261 aatcagcgcc acaaacagag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg
     1321 cttacgcctc caccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcatgt
     1381 tcaccaccgg cagcacctcg catccccgcg caacaaacag agagagcgca tcgcgtagcg
     1441 tcatctcttc tgccagtgcc tcaccatctg ctcgttcttc gcgacgcacg taatccgcca
     1501 cactacgtaa cgaaagcagg cgcacaccca gttcactacg tccaaaaaac tggcggacaa
     1561 aatcattcgc cggacgagtc agcatcgtca gcggattgcc ctgctgcact acttcaccgt
     1621 gatccatcaa taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca
     1681 gcacaatggt acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc
     1741 gcgttaccgg gtccagtgcg ccaaaaggtt catccattag taagacttgc ggatcggcag
     1801 ccagtgcacg cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac
     1861 gctcacgcaa atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa
     1921 tccgcgcccg cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc
     1981 agtgggggaa caggccaata gattgaatgg catagcccat ccggcggcgc aactccagta
     2041 ctggcagcga gcgaatttct tctccggcaa agcggatctc tccgctgtca tgctccacca
     2101 ggcggttaat cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg
     2161 aaaaactccc ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca
     2221 gtttgctgac atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa
     2281 tcgaacagcg cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact
     2341 aaatcaatgg cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg
     2401 attaacgccg caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc
     2461 agaaataccg gtaacgccag cggtaactga acatgcagga aacgctgcgc cccgctcatc
     2521 cccatcgctc tggcgctttc cagcacatcg cgcgggatct ggttcaaacc gactaccacg
     2581 ccgcgcacca gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg
     2641 gttcctgcta tgccgagcgt ccccagccac ggaaaggccg taaccagcgc ggcaagcggc
     2701 gcaatcaaca ggccaaagag cgccaccgaa ggcacggtct gaatcacatt gagcagagaa
     2761 aaaattgccc cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc
     2821 actaacgcag gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca
     2881 aacacatcct gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag
     2941 tacagcaacc acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga
     3001 tgcgtggaga ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc
     3061 cagaaaccac tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgggtc
     3121 gccgcctttc cagctcccca caccagcaat acgaagacga attgcgccag aatgagtgca
     3181 caaatgctgc cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc
     3241 accagcatcc agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact
     3301 aaacgattag gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc
     3361 agcagcgcca gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct
     3421 ttttgtttca ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg
     3481 gcattcagtt gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt
     3541 gccatttgcg gatactccct caacaccgac tcacgcacca ctggtgcagg cgcgtagata
     3601 ggttgcacac cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca
     3661 gtgccgtaag ccattgcagc attaacgcca gaggtttgct gggcagcggc tttgatcgtc
     3721 accgccgtgt cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag
     3781 gctttttcaa acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt
     3841 ttgaaggtgc cgccctcttg cagataacga ctcaggtcgg caagcgaagt gagtttattt
     3901 ttctctgcca catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc
     3961 agccagatta acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc
     4021 gcgtttttcc acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat
     4081 tccggataga tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg
     4141 agttgcactt tattcaccgt cggtacgccg tggctttcca gcacctgcaa aatgatattg
     4201 ccgagtagcg caccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt
     4261 agcggcaggc tcacggctgc caacataacc agtgaacctg cccagagctt taagagtggc
     4321 atgatgcttt cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg
     4381 ataatggcgg ggttagcttg agcggaagga ttcagttgca gaatcagata aatacttaag
     4441 aggcatattc ggtccggcgt ttcctgccgg atgcggcgcg agcgccttat ccggcctaca
     4501 aagggcgcaa acgtcgcgcc cttactaaag cattacagca actcaaactc gcctttctta
     4561 acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc caggctcggc gtcatatttc
     4621 atctgttgat tccagaactt cagcgcctca atatcgatcg ggaagctgac agtctgagtt
     4681 tcgcccggtt tcagggtgat tttctcaaag cctttcagct gtttcacagg gcgactcatg
     4741 gaagccgtca catcctgcaa gtacatctgc actaccgtgg caccctcgcg cttaccggtg
     4801 ttcgtcacct gcacgctggc agtcactttg ccgtcacgct tcatggtcgg cgcagaaagt
     4861 ttcacatcag agacggtgaa agtggtgtag ctcagcccat agccgaacgg atacaacgcc
     4921 ccgttagctt catcaaaata acgcgaagtg tatttgttcg gcttgtcggc attatacggg
     4981 cgaccggtat tcagatggct gtagtacacc gggatctgcc cgacagaacg cgggaaggac
     5041 attggcagct tgccggacgg gttgtaatcg ccaaacaata catcggcaat tgcattaccg
     5101 ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag cctgctgatc ttctttcacc
     5161 agcgccagcg gacgcccgtt catcagcacc agcaccagcg gtttaccggt ggctttcagc
     5221 gccacaatca agtcacgttg gctttgcgga atagtgatat cggtccggct ggaggcttcg
     5281 tgcgccatcc cctgtgcttc accgactaca gccaccacca catcagattg tttcgccgtc
     5341 tgcaccgctt catcaatcat ctcttgcggc gaacgcggat cgactttgac cgcttcttca
     5401 tactgattca ggaaatcgat aatgcctttg tcactggtaa cgttcgcccc tttggcatac
     5461 agcactttac cgttttcacc gacggcattt ttaatcccgg tcagtacggt cacggattga
     5521 tcggcaacac cggctgcgga ccagctgccc atcacgtcac gtttactgtc cgccagtggc
     5581 ccaaccaccg caatggtggc cgattttttc agcggtaacg tttcgagacg gtttttcagc
     5641 aacaccaagc tttcgcgcgc cacttcacgc gcttctttac ggtgcaggcg gctttcggca
     5701 ttggtatcca ccgggtcaga ctctttcggc cccaaatggc tgtatgggtc gttaaacaac
     5761 cccatatcat atttaacgtt cagtacatgg cgggcagcat cgtccagctc tgccatcgtc
     5821 actttgccgg atttaatcaa cccaggcaga tacttcgagt agtactcgtc gctcatgctc
     5881 atgttgattc cggatttcag cgccacgcgc accgcatctt ccgggtctgc cgccgtgcca
     5941 tgtttaatca gctctttgat tgcaccgtga tcggaaacgg tgatgccttt aaagccccac
     6001 tggtcgcgca gaacatcttt cagcagccag gaatcggagg tggctggcgt gccgttcagc
     6061 gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca gccccgcttt gtacggcggc
     6121 atataatcat taaacaggcg ctgcggactc atatcgacgg tgttgtactc tttaccgcct
     6181 tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca tcaccgagta gcgatctgcc
     6241 gggcttttac cctgcatcgc ttccaccatg gttttaccca ttgttgaggt gagatacgta
     6301 tcttcgccaa aaccttcgga agcacgtccc cagcgcggat cgcgcgagac atcgaccatc
     6361 ggtgcccagg tcatattcag gccatcatct gccgcttcat aagcagagac acgtccgacc
     6421 gttttcactg catcgaggtt aaaagacgag gccagaccga ggctaatcgg gaacaccgtg
     6481 cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa ttttcaggcg gcttaattcc
     6541 atcacctgat cctgcatggc gcggatatcc tgacgggtta cggtgttgaa aatcgcccca
     6601 acctgaccgt ctttgatcat ctcgcggatc gcctctttcg ggttatccgg gccgacgctg
     6661 attaagcgca gctgaccaat tttctcatca actgtcattt tcttaagcag ttcggtgacg
     6721 aacgcatccc gcgcttcggg cgttaatgga tggttgccga ataaatcatc cgccagtgct
     6781 ggctgcaggg ccagactcac cgcgattcct actgaacata gccatttcat atggattttt
     6841 cttcctttgt tgcccgacgt ggcagcgaaa atggtgcaaa aaccgtagtt tgccataagc
     6901 atgatggaga gagaaaaaga atgctcagtt tattgtctga attttcaaaa tattcactcg
     6961 ctgaattgtt atacaaggcg ctattctagt ttgtgatatt ttttcgccac cacaaggagt
     7021 ggaaaatgtc ttccatgaca acaactgata ataaagcctt tttgaatgaa cttgctcgtc
     7081 tggtgggttc ttcacacctg ctcaccgatc ccgcaaaaac ggcccgctat cgcaagggct
     7141 tccgttctgg tcagggcgac gcgctggctg tcgttttccc tggctcacta ctagaattgt
     7201 ggcgggtgct gaaagcctgc gtcaccgccg acaaaattat tctgatgcag gccgccaata
     7261 caggcctgac cgaaggatcg acgccaaacg gtaacgatta tgatcgcgat gtcgttatca
     7321 tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa gggcgaacag gtgctggcct
     7381 atccgggcac cacgctctat tcgctggaaa aagccctcaa accgctggga cgcgaaccgc
     7441 actcagtgat tggatcatcg tgtataggcg catcggtcat cggcggtatt tgtaacaact
     7501 ccggcggctc gctggtgcaa cgtggcccgg cgtataccga aatgtcgtta ttcgcgcgta
     7561 taaatgaaga cggcaaactg acgctggtga accatctggg gattgatctg ggcgaaacgc
     7621 cggagcagat ccttagcaag ctggatgatg atcgcatcaa agatgacgat gtgcgtcacg
     7681 atggtcgtca cgcccacgat tatgactatg tccaccgcgt tcgtgatatt gaagccgaca
     7741 cgcccgcacg ttataacgcc gatcctgatc ggttatttga atcttctggt tgcgccggga
     7801 agctggcggt ctttgcagta cgtcttgata ccttcgaagc ggaaaaaaat cagcaggtgt
     7861 tttatatcgg caccaaccag ccggaagtgc tgaccgaaat ccgccgtcat attctggcta
     7921 acttcgaaaa tctgccggtt gccggggaat atatgcaccg ggatatctac gatattgcgg
     7981 aaaaatacgg caaagacacc ttcctgatga ttgataagtt aggcaccgac aagatgccgt
     8041 tcttctttaa tctcaaggga cgcaccgatg cgatgctgga gaaagtgaaa ttcttccgtc
     8101 cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct gttccccagc catttaccgc
     8161 cgcgcatgaa aaactggctc gataaatacg agcatcatct gctgttaaaa atggcgggcg
     8221 atggcgtggg cgaagccaaa tcgtggctgg tggattattt caaacaggcc gaaggcgatt
     8281 tctttgtctg tacgccggag gaaggcagca aagcgttttt acaccgtttc gccgctgcgg
     8341 gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt cgaagacatt ctggcgttgg
     8401 atatcgctct gcggcgtaac gacaccgagt ggtatgagca tttaccgccg gagatcgaca
     8461 gccagctggt gcacaagctc tattacggcc attttatgtg ctatgtcttc catcaggatt
     8521 acatagtgaa aaaaggcgtg gatgtgcatg cgttaaaaga acagatgctg gaactgctac
     8581 agcagcgcgg cgcgcagtac cctgccgagc ataacgtcgg tcatttgtat aaagcaccgg
     8641 agacgttgca gaagttctat cgcgagaacg atccgaccaa cagcatgaat ccggggatcg
     8701 gtaaaaccag taaacggaaa aactggcagg aagtggagta aaaattacgg atggcagagt
     8761 atcgccatcc gaattcactt aatcgttctg tgccgtctgc cccgccgccg ccatttgggc
     8821 ggctttttgt tttttatagc tcaacgctgc tgccggcaca ggcatcactt taccggtttc
     8881 aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa acgcgtccat
     8941 cactaccagc gccaccggtt tattattgat aaccgtacgc atcaccagac aatggcccgc
     9001 cgcattggta aagccggttt tggttaactg aatattccag ttatcgcgat acaccagatg
     9061 attagtattg cggaacggca gcgtatacgt cggattagag aaggttgcca tatcttcccg
     9121 ggtagtactt aactgcccga tcaacggata ttgtttgctg gcaatgagca gtttggttaa
     9181 gtcacgggca gttgaaacgt tatgcaccga caatccggta ggttcaacaa agcgcgtgtt
     9241 gttcattccg agcgatttcg ctttcgcatt cattgcctta ataaaggctt tgtaaccacc
     9301 gggataatgg tgcgcaaggc ttgccgccgc gcggttttct gaagacatca gcgccagcaa
     9361 cagcatatct ttacggctga tttcgctatt cagtcgtacg cgcgaataga cccctttcat
     9421 ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc gtgcatccag
     9481 cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca gatccgggtg
     9541 gttcgaatag atcactttgt tggtattcag atcaacaatc atcgcgctac cggaggcaat
     9601 ttccggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg gtgcaaaagg
     9661 cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca tgatgagcat
     9721 tcagatagtg gttcacgcgc acgggttgcg caccgccgga gtaaggattt actgaggcta
     9781 gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag gagaatcgtg aggaaatgct
     9841 gcattgctga catttacgcc agcaatgcaa cgtcaaagaa ctttctagaa caaacgataa
     9901 ccgtagcccc atagtataac ggttagggcg agcagcactt ccagtaccag cacgccaatc
     9961 gccagcgtcg aactagagaa gctaaggcct tcctctttgt tgatattcag gaagctcgga
    10021 ataccaaggt aaagcaggta gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac
    10081 aaccagacca gtggataaag cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag
    10141 cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg gataattacg cgccatccac
    10201 cagatgaccc gccccatcac cgcgacccca gccagcataa cgccgtaaaa caagacagcc
    10261 agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa attccagcca
    10321 atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg
    10381 tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg
    10441 ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga gttcatgaac
    10501 ctgacagttc aagtataagt cagcttgtga ttattttttg ttcgcccatg taattttcac
    10561 tgtctgatga acgtcctttt tcagcgtgaa tgattcacag ggtgtatgct tactggcaac
    10621 caaagggaga cagactggcc tatggatctc aatacactta tctcacaata tggttatgcc
    10681 gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga ctttgctggg aggcgttgcg
    10741 gcgcatcagg ggctattaaa gttcccgctg gtggtacttt ctgtggcgct tggcggcatg
    10801 attggcgacc aggtgctcta tctgtgcggg cggcggtttg gcggcaagct gttacgccgt
    10861 ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg ccatccgtat
    10921 ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg tgattggccc gacgctgatt
    10981 ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg cgcatttgcc
    11041 tgggcgttga tttttaccac tattggttac gctggtggtc aggtgattgc gccgtggttg
    11101 cacaatctcg accagcattt gaagcactgg gtctggttga ttctggtcgt ggttctggtg
    11161 gtgggcgtgc gctggtggct gaaacgacgc gggaagaaaa agccggatca tcaggcgtaa
    11221 aaccattgcc ctggataagg cgttcgcgcc gcatccgaca tccggagtac catgcctgat
    11281 acgacgcttg tgcgtctttt catgccgacc aaaatattac ccaatgaaat atccacgcac
    11341 ctcactgcgc tattctgggt tgaactgtgg attcgccaac ataaagccgc catccactat
    11401 caacgactgc ccggtggtgt aatttgcgcc ctccgaacaa agccacacca ccaggctggc
    11461 aatctcatgc gttgcgccaa aacgccgcaa gggaatcgaa ggctccgcgt cgggcttcac
    11521 gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc ccaggcgcga ctgcgttcac
    11581 caaaatctta tgcctgacca gctccagcgc catcgctttg gttaacccac cgagcgcatg
    11641 tttagcggct gtgtaggcgc tggcatccgg cagcggcgta tgttcatgta ccgacgtaat
    11701 gttgatgatg cgaccgccct gcccttgttt caccatctga cgagccgcaa tttgcgagca
    11761 taagaatgca ccatcgacat caacggtaaa aatcttgcgc cactcatcaa aagccatatc
    11821 aagaaacggc gctttggtca ttgcacccgc attattcacc agcacatcaa tgcgccccag
    11881 ccgttgaatg agtttctcca gcgccagtgc cccttctggt agattgccga gatccagctg
    11941 cacgatctcc gcacgtacgc cgtggctaac tacctcacgc gcggtatctt ttgccccttc
    12001 ttcatctgag tgccaggtaa taccaatatc aaacccctgc tgcgccagta ataacgcgca
    12061 ctctttgccg atccccgaat cggaggcggt aataatcgca acctgtgcca tcgagttctc
    12121 cacttaacgc tgaataaacg ttaagtatag aaggcgcata tcatcagcgt ttgtaacccc
    12181 cgcccaacgc accagtgagt tgaatggagg catccagcca ctgcccttgc aataacaggc
    12241 cattggcccg ctcacgcagc gcggggattc tggcttcgct gacgcgggaa ccagcaatga
    12301 tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg caaggcatcg cgctcaattt
    12361 gcgcctgatg ctggtttttc tccgccagtg tctgaacctg actggctgcc cgcgccacgt
    12421 cattcaccgc ttcaaccacc gctttgttgt agctggcgat agacaagttg ctttcggctt
    12481 ttgcgatatc gagattggcg ttaagacgac cactatcgaa aatgggtagc gtcaggcctg
    12541 ccgtaacgcc catttgctgc gcggaatgac ggaacagatc gcttaagtgc aacgcatcct
    12601 gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc cgcttttgcc gcatcaatgg
    12661 tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa atctgcccgc cgggccagta
    12721 aggagtaccc cagttcatca ggaagctggc ttgccacttt cggcaacgcg accggtttaa
    12781 gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa ccgtgcctca ataattttca
    12841 ttttccccgc gacatcgttg agctgctgcc gggttttgct ggcattaata tcggtttcca
    12901 caccttcaac tgaagaagta atcccgttct gatatagctg gcgatcggtc gcgataatgg
    12961 tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa cgccgcctgg gtttgccact
    13021 cccagtacag gcgggctacg ctgccagcca gcaattggcg ggtttgctcg cgttccgccg
    13081 cccgtgcttt aaccgtaccc aggcgggcag taacctccgc ccgattcttt ccccagatat
    13141 cgagatgcca gcccgccgtt aagccaaaag taccgttggt gtaccacggg ccggtcgtac
    13201 ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc ttctgccgac attttttgcc
    13261 gctccatatc cgccgaaaag tcgatctgcg gaccatcctg agtggcaact gccttcgcct
    13321 gggcttcagc tagctgaatg cgctgttcag ccacctgcat atccggtgcg ttctgtagtg
    13381 cattgttaat taaggaagtg agttgattat cgtgatactc cagccaccat tggctgtctg
    13441 gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac ttgtgcagcg ggcgtttgct
    13501 ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc ggccagcatc agtaacagcg
    13561 gaaaacaggc gatggctgga taaaaggaat cacgattcat gggggaataa tcaggtaaga
    13621 aaaggtgcgc ggagattacc gtgtgttgcg atatattttt tagtttcgcg tggcaataca
    13681 tcagtggcaa taaaacgaca tatccagaaa aatatacact aagtgaatga tatcttccga
    13741 tttatcttaa tcgtttatgg ataacggcaa agggcttcgt tttttcctat acttattcag
    13801 cactcacaaa taaaggaacg ccaatgaaaa ttatactctg ggctgtattg attattttcc
    13861 tgattgggct actggtggtg actggcgtat ttaagatgat attttaaaat taattaatgt
    13921 catcaggtcc gaaaataacg agaatatttc agtctctcat cctgttgcgc tcctgtcatg
    13981 tgcattgctt catata
//
LOCUS       DAZUGK010000114        13898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZUGK010000114 DAZUGK010000000
VERSION     DAZUGK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.115"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..755
                     /gene="cbrA"
                     /locus_tag="ACHY5W_002998"
     CDS             <1..755
                     /gene="cbrA"
                     /locus_tag="ACHY5W_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFS4447865.1"
                     /translation="VYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTCFTSPTSRM"
     gene            complement(716..829)
                     /locus_tag="ACHY5W_002999"
                     /pseudo
     CDS             complement(716..829)
                     /locus_tag="ACHY5W_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            965..1621
                     /locus_tag="ACHY5W_003000"
     CDS             965..1621
                     /locus_tag="ACHY5W_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447866.1"
                     /translation="MKLNFKGFFKAAGMCSLAVSLTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGGEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKASDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(1665..2477)
                     /gene="yidA"
                     /locus_tag="ACHY5W_003001"
     CDS             complement(1665..2477)
                     /gene="yidA"
                     /locus_tag="ACHY5W_003001"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4447867.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(2592..2990)
                     /gene="yidB"
                     /locus_tag="ACHY5W_003002"
     CDS             complement(2592..2990)
                     /gene="yidB"
                     /locus_tag="ACHY5W_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFS4447868.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            3108..3206
                     /locus_tag="ACHY5W_003003"
                     /pseudo
     CDS             3108..3206
                     /locus_tag="ACHY5W_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3230..5644)
                     /gene="gyrB"
                     /locus_tag="ACHY5W_003004"
     CDS             complement(3230..5644)
                     /gene="gyrB"
                     /locus_tag="ACHY5W_003004"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4447869.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(5673..6746)
                     /gene="recF"
                     /locus_tag="ACHY5W_003005"
     CDS             complement(5673..6746)
                     /gene="recF"
                     /locus_tag="ACHY5W_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4447870.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERDTAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(6746..7846)
                     /gene="dnaN"
                     /locus_tag="ACHY5W_003006"
     CDS             complement(6746..7846)
                     /gene="dnaN"
                     /locus_tag="ACHY5W_003006"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4447871.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(7851..9254)
                     /gene="dnaA"
                     /locus_tag="ACHY5W_003007"
     CDS             complement(7851..9254)
                     /gene="dnaA"
                     /locus_tag="ACHY5W_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4447872.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            9551..9631
                     /locus_tag="ACHY5W_003008"
     CDS             9551..9631
                     /locus_tag="ACHY5W_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447873.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            9861..10001
                     /gene="rpmH"
                     /locus_tag="ACHY5W_003009"
     CDS             9861..10001
                     /gene="rpmH"
                     /locus_tag="ACHY5W_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4447874.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            10018..10377
                     /gene="rnpA"
                     /locus_tag="ACHY5W_003010"
     CDS             10018..10377
                     /gene="rnpA"
                     /locus_tag="ACHY5W_003010"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4447875.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            10341..10598
                     /gene="yidD"
                     /locus_tag="ACHY5W_003011"
     CDS             10341..10598
                     /gene="yidD"
                     /locus_tag="ACHY5W_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4447876.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            10601..12247
                     /gene="yidC"
                     /locus_tag="ACHY5W_003012"
     CDS             10601..12247
                     /gene="yidC"
                     /locus_tag="ACHY5W_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4447877.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            12353..13717
                     /gene="mnmE"
                     /locus_tag="ACHY5W_003013"
     CDS             12353..13717
                     /gene="mnmE"
                     /locus_tag="ACHY5W_003013"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4447878.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         3204 a         3742 c         3617 g         3335 t
ORIGIN      
        1 aggtttatca cgacagcctg tgtcgaaaaa tctggcgtga ggatgataaa tggcatgtca
       61 tttttcgtgc agacggttgg gagcagcata ttaccgcccg ctatctggtc ggtgccgatg
      121 gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg
      181 ctatccagca gtggttcgca gagaaacatc ctgtgccgtt ctactcctgc atctttgata
      241 atgcgataac tgactgttac tcatggagta tcagcaaaga cggttatttt atctttggcg
      301 gtgcctatcc catgaaagac ggtcagacgc gtttcacgat gctgaaagag aaaatgagcg
      361 cctttcagtt ccagtttggt aaggcggtga aaagcgaaaa atgcacggtg ctgtttccct
      421 cgcgctggca ggattttgtc tgcggtaagg acaacgcctt tctgattggt gaagcggcgg
      481 gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc gcagagattc
      541 tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg cgcgccaccc
      601 gcaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg
      661 ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg aaagattatc
      721 caacgtgctt cacatcgccc accagcagga tgtaagagag cgcgccaatc aacgcgacgg
      781 cggagatata aaccagtgcc ggggcgaacc catagccttg caacagatat tagtccacaa
      841 aaaaacacct cataagaaaa ggtcttatat cgggcagaaa tctgaaaagg gatatcaggc
      901 gctaatcagg agggaaagaa gagtatgctt tcaacggctt agctactcgt ttaaaggatt
      961 aatcatgaag ttgaatttta agggattttt taaggctgcc ggtatgtgtt ctttggcagt
     1021 ttcgttgacc ggatgtatca cctggggctt ggtttccaat actgcaagcc gtccacatgc
     1081 cgagcaatgg cggtcagaca cgattaaggg tttatcgctg gcagaggata gtaacggtac
     1141 gaaaggctat gtttttgttg gtgaatcgtt ggactatctg ttgaccaccg gcggcggtga
     1201 ggtggttaaa atgctgaacg atccggcaat tcatggtgaa agaatcaccg tttctgataa
     1261 cgcgaaattt attttaagca gcagtaataa gaatttctct ggtgctatta cgctctatta
     1321 cgactggaac aacgaagaag ataaggcact ggcaacacaa tatggtttta tttgtgatac
     1381 ccggcgttgt acatggatgc tggacggcct gaaaggctct atccaccaga aaaataaaaa
     1441 agcggattac tcaaacgtga tggttttcca tcaacccttt acggtgggat tttatgagta
     1501 caaagcatca gatggcgttc cgcgcggcct cgttaatgcg ctgctaccag tcactcttac
     1561 gctggatata gtaacgtcac cactccagtt cctgatttta tgcactacca gaaattgtta
     1621 atcgctgcgg gcaaacgcgt tcatgccgtt tgcccgccca cagattaatt cagcacatac
     1681 ttctcaatag caaacgccac gccatcttca aggttggatt tggtgacaaa gttcgccact
     1741 tccttcaccg aaggaatagc gttatccatc gccacaccga cgcctgcata ttcaatcatt
     1801 gcgatgtcgt tttcctgatc gccaatcgcc atgatttctt ccggtttaat acctaacacg
     1861 tcggccagtg atttcacacc cgtacccttg ttaacgcgtt tatcgaggat ttcgaggaag
     1921 tacggcgcac ttttcagcac ggtatatttc tctttcactt cctgcggaat acgcgcgata
     1981 gcctggtcga ggatggcggg ttcatcaatc atcatcactt tcaggaactg ggtattgggg
     2041 tccattttct ccgcttcgca gaacaccagc ggaatggtgg caacgaagga ttcatgcacc
     2101 gtgtagtagc tgatatcacg gttggcggtg tacagcgtgg tgcggtccag tgcgtggaaa
     2161 tgagaaccga cttcgcgaga gagtttttcc aggaaacggt agtcgtcata gctgagagcg
     2221 gtttgcgcca cagtgctacc atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg
     2281 cagtagtcgc ccggctgttc catatgcagc tctttcaggt aattgtgaac acctgcataa
     2341 gggcgacccg tcgttagcac gacattcacg ccacgggcgc gagctgcggc aatcgcattt
     2401 ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg tgccatccat atcgatagca
     2461 atgagtttga tagccatgag ttccccaggt agattggttc ctgacccatg ctaacgcgat
     2521 tccgctcaaa aatcagtaca acacccgagg gaaaaggggg atgcaacgcg ggttgcatgc
     2581 tccccatttg cttagcggaa gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga
     2641 tcgttattgg cttgtggtga cacttcacct tgcggtgaga gcgcatcaat aatcttcggc
     2701 aattgttctg ccagtaaact ggaagctgta ctggtatcca cgccaagttt ttgcccgaga
     2761 tcggacaccg catttgtgcc gagcgccgat tccagttgtt cgccgctcac cgattgattg
     2821 ccctgttgat tactcagcca ggttgagaga atggccccta agccgccact ttgcagtttt
     2881 tccagcagca cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc ctgatatttc
     2941 cccgcatcgc ctttcagaaa ggcgccgaca acttcatcaa aaagccccat gataatcacc
     3001 tgtaaagcgt tacgtgttga cccaaaaagt atagaactgc gggatgataa ttgcggattg
     3061 cagaaataaa aagggcggag atgatctccg cccttttctt atagcttctt gccggatgcg
     3121 gcgtgaacgc cttatccggc ctacaaaaac atgcaaattc aatatattgc aggggtctcg
     3181 taggcctgat aagcgtagcg catcaggcac gctcgcatgg ttaacgccat taaatatcga
     3241 tattcgccgc tttcagggcg ttctcttcaa taaacgcacg gcgcggttca acggcgtcgc
     3301 ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc cttaacggta acgcgcagca
     3361 tacgacggct ttccgggtcc atggtggttt cccacagctg ttccgggttc atctcgccca
     3421 gacctttata acgctggatg gagaggccgc gacgggactc tttcaccagc cagtccagcg
     3481 cctgctcgaa gctggctacc ggctgacgac gctcgccacg ttcgataaac gcatcttctt
     3541 ccagcaagcc acgcagtttc tcacccagcg tgcagatacg acgatattcg ccaccggtga
     3601 taaactcgtg atccagcgga tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg
     3661 gttcaaacag gttttgttcg gcattggtgt ggacatcaaa cttccactgg ctgccgtgct
     3721 gttctttgtc gttcagttcg ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat
     3781 cagagaggtc ggcttccgtc agcgtcggct gatagataag ctctttcagc attgctttcg
     3841 gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc gttgtactca gacaccagtt
     3901 tctctaacgc ttcgccagcc aatgccggtg cgctggcgtt ggtgtgcagc gttgcgccgt
     3961 ccagcgcgat agagatctgg tactgatcca tcgcttcgtc gtctttaatg tactgttcct
     4021 gcttgccttt cttcactttg tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa
     4081 caatttccgg catctgacga tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt
     4141 cgacgtccgc atcggtcatg atgatgatgc tgtgataacg cagtttgtcc gggttgtact
     4201 cgtcacgacc gataccacaa ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga
     4261 gcatcttatc gaagcgcgct ttctcgacgt tgaggatttt acccttcagc ggcagaatcg
     4321 cctggttctt gcggttacgc ccctgcttcg cagagccgcc cgcggagtcc ccttccacaa
     4381 ggtacagttc agagagcgcc ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc
     4441 cagctaagtc gagcgcacct ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac
     4501 gggcacgggc agcatcgata attttgccaa ctacgatttt cgcgtcggtt gggttttcca
     4561 gcaggtactc cgccagcagt tcgttcatct gctgttctac cgccgatttc acctcagaag
     4621 aaaccagttt gtctttggtc tgggaggaga atttcgggtc cggcactttc acggaaacga
     4681 ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct gactttggct tttttgctgt
     4741 agccttcttt gtccatgtag gcgttcaggg tacgggtcat cgccgcacgg aagcctgcca
     4801 ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga
     4861 agccatcgtt ccactgcaac gccacttcga cgccgatacc gtctttttcg gtggagaagt
     4921 agaagatatt cggatggatc ggcgttttgt tcttgttcag atattcaacg aacgccttga
     4981 tgccgccttc atagtggaag tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg
     5041 aaacgccgga gttgaggaac gacaactcac gcagacgttt cgccagaatt tcatactcga
     5101 actcggtcac attggtgaag gtttcgaggc tgggccagaa acgcaccatg gtgccggttt
     5161 tttcagtctc gccggtaacc gccagcgggg cctgcggtac accgtgttcg tagatctgac
     5221 ggtgaatttt accctcgcgc tggataacca gctccagttt ttgcgacagg gcgtttacta
     5281 ccgaaacacc aacgccgtgc agaccgccgg acactttata ggagttatcg tcaaatttac
     5341 cgcctgcgtg cagaacggtc atgatcactt ccgccgccga tacgccctct tccgggtgaa
     5401 taccggtcgg aatgccgcgc ccgtcatcct gtacagagac agagttatcg gcgtgaatgg
     5461 tgacgataat ttctttacag tgacccgcga gcgcttcgtc gatagcgtta tctaccacct
     5521 cgaataccat gtggtgcaga ccggtgccgt catccgtgtc gccgatatac atacccgggc
     5581 gcttacgcac cgcatccagc cctttcagga ctttgatact ggaggagtca taagaattcg
     5641 acatcaacgt ttctcgctca tttatacttg ggttaatccg ttattttacc cttttccacg
     5701 gtaaacatct tcgaattttc gtccgacatg tctataacgt gttcagcact gatcgcgctg
     5761 acaaagacct gcgattgcgt cgcttttaag cggctggcaa gcagcccgcg acgctcatca
     5821 tcaagctcag aggcaaaatc atctatcagg tagagacacc gccgcccgct ttcacgggtg
     5881 aggaactctc cttgcgccag acgtaaggcg cacatcaaca gcttaagctg cccacgcgat
     5941 aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta agtccgcttt atgcgggcca
     6001 tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac gttccagcac ctcagcatat
     6061 tctgtctctt tctcccagcc gcgctggaaa gagaaagtca gagaaaactc agggagaaat
     6121 tgcttacagg tatcagccat atcggccgcg ataccggcgc tatactccgc gcgccaggtg
     6181 ctgatttgct ccgccagcgg gatcagttct ttatcccacg ggcgtagctg ttcgtaacgt
     6241 gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc gcttgagatt gctccaggcg
     6301 gtgaaaaatc cgggttcgtt gtgaaagcat ccccagtcga ggaatgctct tctgtatttg
     6361 gggccgccgt tgagtaaagt aaacccttct ggcgttatca gctgcattgg catcaggtgc
     6421 gccagttccg cgactttatg cccgtcagta ccgtcgatgc ggactttgct gtcgccctgt
     6481 ttgtctttgg ttaagccaat cgccgtatcg cgctcttcgc cctgtaatcg cccgtggaga
     6541 acaaacgcct cctgctcatg gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga
     6601 ccatggccga gcgtatagat ggcttccagc acgctggttt tgccactgcc gttggcacct
     6661 accagaaagt taaagccggg agataaagcg agatccgcgg tttcaatgtt gcggaaatcg
     6721 cggatcaaca agcgggtgag ggacattaca gtctcattgg catgacaaca taagccgcgc
     6781 tctggctggc tgcatcttca atctgcacgc tggaaaccga atcggtcagc atcatgcgga
     6841 cgttttcgca tttcagcgcg ttcagaacat ccagcacata gctgacgttg aagccgattt
     6901 ccatctccgc accgctatag gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt
     6961 tgttggcggt gattttcagc tggttttcgc tgacatacag gcgcacaccg cggaatttct
     7021 cgttagagag aattgccgcg cgagcaaacg cctgcttgag gagatcgcag cctgcttcca
     7081 gatgtttgtc cgggttcttc ggcagaacgc ggcgataatc cgggaagcga ccatccacca
     7141 gtttggaggt gaagataaag tcgccaacgt gggcgcgaat gttgttgctg ccaatctgca
     7201 cgcgcagcgg attatcgccg ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg
     7261 gcacgatcac cgaatggctt ggcaaagatt gaccaattgg cattgaacag actgccagac
     7321 ggtgaccatc ggtcgccacg gtacgcagtt cttcaccttc ggtttcaaac agcataccgt
     7381 ttaagtaata gcgaacgtcc tgatgcgcca tcgaaaactg ggtcgcttca atcagacgct
     7441 tcatcgtcgc ctgcggcagg gtaaattcga cttcactctg ccagtcatcg aggttcggga
     7501 aatccgccgc tggcagggta gacagcgaaa aacggctacg cccggagcgc accagcatcc
     7561 gctcaccttc cagctgcacg gcaatttccg cgccttcagg cagaccacgg cagatatcaa
     7621 agaatttgcg cgccggaacg gtcgtcgccc ctggctcgtg tggctgaacc agcgcaacgc
     7681 gtgccaccat ttccatctcg agatcagtac cggtcagcga caacgtaccg tcagcaacct
     7741 gtaacagcag attaccgaga atcggtagcg taggacgacc acctaacgga ccgctcacct
     7801 gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa tttcataggt ttacgatgac
     7861 aatgttctga ttagatttga aaaatcttct ttgatatcgt ggctctcttc acgcagctgc
     7921 tcgatcttcc ggcaggcatg aagcaccgtc gtgtggtcac gaccaccaaa cgcatcgcca
     7981 atctccggca gactgtggtt agtcagctct ttcgccagcg ccatcgccat ctgacgcgga
     8041 cgagccaccg agcgggatcg acgcttggaa aggagatccg cgactttgat cttgtagtac
     8101 tccgccaccg tcttctgaat attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag
     8161 tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta
     8221 gcaataacgc ggttcagcgc gccttccagc tcacgtacgt tagatcgtag acgcttggcg
     8281 ataaagaacg ccacttcacc tggcaaacga atgtcatttt cgtcggcctt tttcatcagg
     8341 atcgccacgc gggtttctaa ctctggcggt tcgatcgcca cagtcagccc ccagccgaag
     8401 cgggatttca aacgatcctc aacgccgttg atctctttcg gatagcgatc cgaggtgaga
     8461 atgatctgtt gattaccttc cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat
     8521 cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca gtgcatctac ggaacggtag
     8581 tagcgtttaa actcttcgat cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag
     8641 cgctcggagt gcatataaac cactttggca ttcggcttgc gcgccataat gccgttcccc
     8701 accgcatgca gcaggtgagt tttacccaga cccgtgccgc cataaaggaa caacgggtta
     8761 taggcaccgc cagggttatc cgccacctgg cgagccgccg cgcgcgccag ttggttagat
     8821 ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt
     8881 tctgccgggg ccgggacgtt atcccaacct gagcgcgtag aaggcgcagc acgttgcggc
     8941 tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt
     9001 tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa
     9061 ctggttagca gtccattgat attattaagg tacttgtccc gtacccaatc gaggacaaaa
     9121 cggtttggcg cgtacagggc cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc
     9181 cacatactga attctgtggc tggtaactca tcctgcaatc gggcaagaca ctgctgccaa
     9241 agcgaaagtg acacggcgga ctccactcga acaaaagtcg ataatgacta aggctgaaac
     9301 attcatgatt gttgacgtac gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg
     9361 tctgcggtta tatgcccgat caagatcctg caaaacgatc gggaccgcgg atcatagcct
     9421 aaactgcgca agagatcttc tgtttctcac agattttccc gatttatcca caggatgatc
     9481 cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg cacgatttac gccgcatatt
     9541 ggaaaattta atgaccatag acaaaaattg gcttaatcga tctaataaag atccaggacg
     9601 atccttgcgc tttacccatc agcccgtata atcctccacc cggcgcgcca tgctggtttt
     9661 cactggtgtg aggccgtacg ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag
     9721 gaaagagaat tgactccgga gtgtacaatt attacaatcc ggcctcttta atcacccatg
     9781 gcttcggtgt ccatcgtttc atttttcggc ggatatccaa taaagccatt gaatttattc
     9841 aagtttaggt agaaatcgcc atgaaacgca cttttcaacc gtctgtactg aagcgcaacc
     9901 gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc
     9961 gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta ataaagctaa cccctgagtg
    10021 gttaagctcg catttcccag ggagttacgc ttgttaactc ccagtcaatt cacattcgtc
    10081 ttccagcagc cacaacgggc tggcacgccg caaattacca ttctcggccg cctgaattcg
    10141 ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa
    10201 cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg
    10261 gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc
    10321 tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag
    10381 ccctcattcg ggtctatcaa cgcctgatta gtccgctact cgggccgcat tgtcgtttca
    10441 ctccaacctg ttcaagctac ggaattgagg cattgcgcag gtttggagtg ataaaaggca
    10501 gttggttgac ggtgaaacgc gtattaaaat gccacccttt acaccctggt ggtgacgatc
    10561 ccgtcccgcc cggaccattt gataccagag aacactaacg atggattcgc aacgcaatct
    10621 tttagtcatc gctttgctgt tcgtgtcttt catgatctgg caagcctggg agcaggataa
    10681 aaacccgcaa cctcaggccc aacagaccac gcagacaacg accaccgcag cgggtagcgc
    10741 cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg atctcggtta agaccgacgt
    10801 gcttgatctg accatcaaca cccgtggtgg tgatgttgag caagctctgc tgcctgctta
    10861 cccgaaagag ctgaactcta cccagccgtt ccagctgctg gaaacttcac cgcagtttat
    10921 ttatcaggca cagagcggtc tgaccggtcg tgatggcccg gataacccgg ctaacggccc
    10981 gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact
    11041 gcaggtgccg atgacgtata ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct
    11101 gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc
    11161 gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc actctgccac cgcatctcga
    11221 taccggaagc agcaacttcg cactgcacac cttccgtggc gcggcgtact ctacgcctga
    11281 cgagaagtac gagaaataca agttcgatac cattgccgat aacgaaaacc tgaacatctc
    11341 ttcgaaaggt ggttgggtgg caatgctgca acagtatttc gcgacagcgt ggatcccgca
    11401 taacgacggt accaataact tctataccgc taatctgggt aacggcatcg ccgctatcgg
    11461 ctataaatct cagccggtac tggttcagcc tggtcagact ggcgcgatga acagcaccct
    11521 gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt gctccgcacc tggatctgac
    11581 cgttgattac ggttggttgt ggttcatctc tcaaccgctg ttcaaactgc tgaaatggat
    11641 ccatagcttt gtgggtaact ggggcttctc cattatcatc atcaccttta tcgttcgtgg
    11701 catcatgtac ccgctgacca aagcgcagta cacctccatg gcgaagatgc gtatgctgca
    11761 gccgaagatt caggcaatgc gtgagcgtct gggcgatgac aaacagcgta tcagccagga
    11821 aatgatggcg ctgtacaaag ctgagaaggt taacccgctg ggcggctgct tcccgctgct
    11881 gatccagatg ccaatcttcc tggcgttgta ctacatgctg atgggttccg ttgaactgcg
    11941 tcaggcaccg tttgcactgt ggatccacga cctctcggca caggacccgt actacatcct
    12001 gccgatcctg atgggcgtaa cgatgttctt cattcagaag atgtcgccga ccacagtgac
    12061 cgacccgatg cagcagaaga tcatgacctt tatgccggtc atcttcaccg tgttcttcct
    12121 gtggttcccg tcaggtctgg tgctgtacta tatcgtcagc aacctggtaa ccattatcca
    12181 gcagcagctg atttaccgtg gtctggaaaa acgtggcctg catagccgcg agaagaaaaa
    12241 atcctgattc ggtgagtttt cgctaaaata agggcggtca gttgaccgcc ttttttcttt
    12301 tcgtagggcg gataagcacc gcgcatccgc cacacaaagc aacaggaaca tcatgagcga
    12361 taatgacact atcgtagccc aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg
    12421 catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc
    12481 gcgctacgcc gattatcttc cgtttaaaga cgccgacggc agcgtgctcg atcaggggat
    12541 tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa gatgtgctgg aactgcaggg
    12601 tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc attctgacca ttcccggcct
    12661 gcggattgct cgccctggtg agttttccga acgcgcgttt cttaacgata aactcgactt
    12721 agcccaggcc gaggcgattg ccgatcttat cgacgccagt tcggaacagg cggcccgttc
    12781 ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt aatcatctgg tagaagccct
    12841 cacccacttg cgcatttacg ttgaagcggc aattgatttc cccgatgaag agatcgattt
    12901 cctctccgac ggaaaaattg aagcccagct taatgacgtg attgccgatc tcgatgcagt
    12961 gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg atgaaggtgg tgattgccgg
    13021 acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat
    13081 cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt gagcatatcc atattgacgg
    13141 aatgccgctg catatcatcg ataccgccgg gctacgtgaa gccagtgacg aagtggaacg
    13201 tattgggatc gagcgcgcgt ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt
    13261 cgatggcacc acgacagacg ccgttgatcc ggcagagatc tggccggaat ttatcgcccg
    13321 tctgcccgcg aaactgccga tcaccgtggt gcgcaataaa gccgatatca ccggcgaaac
    13381 gctgggaata agtgaagtga acggtcacgc gttaattcgt ctgtctgcaa ggactggcga
    13441 aggcgtggac gtgctgcgta accatctcaa acagagcatg ggctttgaca ccaacatgga
    13501 aggcggcttc ctggcgcgcc gtcgccacct acaggcgctg gaacaggcag cggagcattt
    13561 gcagcaaggt aaagcgcaac tgttaggtgc ctgggcaggc gaactactgg cagaagagtt
    13621 gcgcctggcg cagcagaact taagcgaaat caccggggaa tttacctctg acgacctgct
    13681 ggggcggatt ttctccagct tctgtattgg taagtaaccg cgcttacgaa gccgcattct
    13741 gactgtcaga tgcggcttcg cttcattatt agcactcctg ttattcctca accctttttt
    13801 taaacattaa aattcttacg taatttatac tctttaaaaa aagcatttaa tattgcgtcc
    13861 cgaacgattg tgattctatt cacatttaaa caatttca
//
LOCUS       DAZUGK010000115        13854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZUGK010000115 DAZUGK010000000
VERSION     DAZUGK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.116"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(189..1076)
                     /gene="rarD"
                     /locus_tag="ACHY5W_003014"
     CDS             complement(189..1076)
                     /gene="rarD"
                     /locus_tag="ACHY5W_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFS4447879.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1128..1595)
                     /gene="yigI"
                     /locus_tag="ACHY5W_003015"
     CDS             complement(1128..1595)
                     /gene="yigI"
                     /locus_tag="ACHY5W_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFS4447880.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1760..2629
                     /gene="pldA"
                     /locus_tag="ACHY5W_003016"
     CDS             1760..2629
                     /gene="pldA"
                     /locus_tag="ACHY5W_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFS4447881.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2762..4591
                     /gene="recQ"
                     /locus_tag="ACHY5W_003017"
     CDS             2762..4591
                     /gene="recQ"
                     /locus_tag="ACHY5W_003017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4447882.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4655..5275
                     /gene="rhtC"
                     /locus_tag="ACHY5W_003018"
     CDS             4655..5275
                     /gene="rhtC"
                     /locus_tag="ACHY5W_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFS4447883.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5337..5957)
                     /gene="rhtB"
                     /locus_tag="ACHY5W_003019"
     CDS             complement(5337..5957)
                     /gene="rhtB"
                     /locus_tag="ACHY5W_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFS4447884.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6068..7090
                     /gene="pldB"
                     /locus_tag="ACHY5W_003020"
     CDS             6068..7090
                     /gene="pldB"
                     /locus_tag="ACHY5W_003020"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFS4447885.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7098..7898
                     /gene="yigL"
                     /locus_tag="ACHY5W_003021"
     CDS             7098..7898
                     /gene="yigL"
                     /locus_tag="ACHY5W_003021"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFS4447886.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHSELEVIGTNAEDAVPHYLRKLYLS"
     gene            7974..8873
                     /gene="bioP"
                     /locus_tag="ACHY5W_003022"
     CDS             7974..8873
                     /gene="bioP"
                     /locus_tag="ACHY5W_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFS4447887.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIACFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8761..9714)
                     /gene="metR"
                     /locus_tag="ACHY5W_003023"
     CDS             complement(8761..9714)
                     /gene="metR"
                     /locus_tag="ACHY5W_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFS4447888.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSI
                     DNTLLLIQMVAARMGIAALPHWVVESFERQDLVATKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9950..12211
                     /gene="metE"
                     /locus_tag="ACHY5W_003024"
     CDS             9950..12211
                     /gene="metE"
                     /locus_tag="ACHY5W_003024"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4447889.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNAILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12496..12741)
                     /locus_tag="ACHY5W_003025"
     CDS             complement(12496..12741)
                     /locus_tag="ACHY5W_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4447890.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12731..13174)
                     /locus_tag="ACHY5W_003026"
     CDS             complement(12731..13174)
                     /locus_tag="ACHY5W_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HFS4447891.1"
                     /translation="MRLPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTTDGVTINR
                     EGIAIVEKHIARFGHDPVNEVMINRLKDIKKGKIPPEQVDLNFYTHECREYQRYCNLG
                     WETGQPDGDAGYALWNHTHTATLEDYKLKGELNDLYHQDALDYDN"
     gene            complement(13218..13853)
                     /gene="tssD"
                     /locus_tag="ACHY5W_003027"
                     /pseudo
     CDS             complement(13218..13853)
                     /gene="tssD"
                     /locus_tag="ACHY5W_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
BASE COUNT         3295 a         3458 c         3643 g         3458 t
ORIGIN      
        1 agggttaagt tttgggtttt gtcaaatcct tggtagatat acgatgtctc taggatggag
       61 taaatactgg ggagttttgg gggtatttcc tgtaacaaat attgttcttt ttcttatctt
      121 aatttttttc aaaaaaagat aaataagtca gtgggtaggc tctttaaacg gcctacccat
      181 gttaattctt aactttttct acgctgcgta taaatcgcat ccatcacaaa aattgccagc
      241 gccacccaaa taaaggcgaa agtcaccatc ttatcggcac ccggtttttc accataaaac
      301 gtcacagcca gcaggaacat cagcgtcggg ccaatgtact ggaaaaagcc taacgttgag
      361 agacgcaagc gcgtggcagc ggcagtaaaa cacaacagcg gtacggtagt gacaataccg
      421 gcggcgatca gcagtaaatt cagcgacatc gggttttgcc ccatatggct ggttgagctg
      481 tcggcaatag caaacaggta aatcgccgcc acgggcagca gccacatggt ttcaattaac
      541 atgccggttt gtgcttctac cgcaatcttc ttgcgcacca ggccgtagaa ggcaaaacta
      601 aatgccagcc ccagcgcgat aataggtagc gaaccaaaag tccacagctg gaccaacacg
      661 ccacatatcg ccagaatcac cgccagccat tgcatccggc ggaatcgctc gccgaggaaa
      721 atcatcccca gcacaatgtt caccagcggg ttaataaagt aaccaaggct cgcttccagc
      781 atatggtgat tgttcaccgc ccagataaac agtagccagt tgccaccaat cagcacggca
      841 gagactgcca gcataaaaat tttctgtggc gtctgaatca gcgtttttaa ataggaccac
      901 tggcggcaaa tgctcatcag caccaccata aagaaaaacg accagatcac gcgatgcgtc
      961 aggatttcat cggcgggcac gtagtaaatc aacttgaagt acgctggggc tataccccaa
     1021 ataaaataag cggcaagagc gagtaatacg ccctgccgcg tttgttttgc atccatcggg
     1081 aatactcatt tttaattggt aacagcagtt tacctgcttt tatgtcttca acctaccata
     1141 taggtggcgg tggcactggc aatataaagc tgttcttcat tgtgtaattc aacgcgggcg
     1201 acggcgactt tattgcctgc acgcaacagg ctactggttg cagtaaaacg ctcgcccctg
     1261 cctgggcgca gataatcaac gcgaagatca atggtcccca tccgcgatag ccgctggcgt
     1321 agttcatctt cactgatggt ttcgtggcgg gttaaggtac ttcccacgca caccagacct
     1381 gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca aaatgctttg cgcccagtta
     1441 cccaccatca tcggctgatt tttaaaggcc agctgtgcga actctttttc gtaacgctcc
     1501 agttccatcc ccaatgcgcg gttaaatggc atgtgataaa caaacatctc acccactaat
     1561 ttcagggctt gttcagcggt cagtacggca gacatatcat ccttacactt cattggttaa
     1621 tgaaatgttg attttatgct tctttgttgt tggtttctac tttaggaagg gataactaac
     1681 ggcaatggag ttaagtatgt aaaatagccc gcagaaaaac attcacctta tcaataattc
     1741 gttacggaga acacgaccga tgcggactct gcagggctgg ttgttgccgg tgtttatgtt
     1801 gcctatggca gtatatgcac aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt
     1861 gcgtggcagt attatcgcca atatgctgca ggagcatgac aatccgttca cgctctatcc
     1921 ttatgacacc aactacctca tctacaccca aaccagcgat ctgaataaag aagcgattgc
     1981 cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc
     2041 atttccgctg tggcgtggga ttttaggccc gaactcggtg ttgggtgcgt cttatacgca
     2101 aaaatcctgg tggcaactgt ccaatagcga agagtcttca ccgtttcgtg aaaccaacta
     2161 cgaaccgcaa ttgttcctcg gttttgccac cgattaccgt tttgcaggtt ggacgctgcg
     2221 cgatgtggag atggggtata accatgactc taacgggcgt tcagacccga cctcccgcag
     2281 ctggaaccgc ctttatactc gcctgatggc agaaaacggt aactggctgg tagaagtgaa
     2341 gccgtggtat gtagtgggta atactgacga taacccggat atcaccaaat atatgggtta
     2401 ctaccagctt aaaatcggct atcacctcgg tgatgcggtg ctcagtgcga aaggacagta
     2461 caactggaac accggctacg gcggcgcgga attaggctta agttacccga tcaccaaaca
     2521 tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa tcgctcatcg actataactt
     2581 caaccagacc cgtgtcggtg tgggggttat gctaaacgat ttgttttgat gaacggttga
     2641 gtggttggca aatctggaat ccagcatcca ggattaccct ctcagagact aaaagcattg
     2701 cagtttctcg cgcaggcgct gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa
     2761 tgtggcgcag gcggaagtgt tgaatctgga gtccggagct aaacaggttt tacaagaaac
     2821 ctttggctac caacagtttc gccccggcca ggaagaaatt atcgacactg tgctttccgg
     2881 tcgcgattgc ctggtcgtca tgcccaccgg tggcggaaaa tccctttgct atcaaattcc
     2941 tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga
     3001 tcaggtggat caactgcaag ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac
     3061 ccgcgaacag caacttgaag tgatgacagg ctgccgcacc gggcaaattc gtctgcttta
     3121 tatcgccccg gaacgcctga tgctggataa ctttcttgag catctggcgc actggaatcc
     3181 ggtgttatta gccgttgatg aagcgcactg tatctcccaa tggggccacg atttccgccc
     3241 ggaatatgcc gcgctcggtc agttgcgcca gcggttcccg acgctgccat ttatggcgct
     3301 gaccgccaca gccgacgaca ccacgcgcca ggatatcgtg cgcctgctgg ggctgaacga
     3361 tccgctgatt caaatcagca gttttgaccg tccgaatatt cgctacatgc tgatggagaa
     3421 attcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa cagcgcggta agtccggcat
     3481 tatctactgc aacagccggg cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg
     3541 tattagcgcg gcggcctatc atgccgggct ggaaaataat gtccgcgccg acgtgcagga
     3601 gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat
     3661 caataaacca aacgttcgct tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta
     3721 ttatcaggaa accggtcgcg ccgggcgtga tggcctgcct gcggaagcga tgctgtttta
     3781 cgatccggct gatatggcgt ggctgcgccg ttgtctggaa gagaagccgc aggggcagtt
     3841 gcaggatatc gagcgccaca aactcaatgc gatgggcgcg tttgccgaag cgcaaacttg
     3901 ccgtcgtctg gtattgctga actattttgg cgaagggcgt caggagccgt gcgggaactg
     3961 cgatatctgc ctcgatccgc cgaaacagta cgacggttca accgatgctc agattgccct
     4021 ttccaccatt ggacgtgtga atcagcggtt tgggatgggt tatgtggtgg aagtgattcg
     4081 tggtgctaat aaccagcgta tccgcgacta tggtcatgac aaactgaaag tctatggcat
     4141 gggccgtgat aaaagccatg aacattgggt gagcgtgatc cgccagctga ttcacctcgg
     4201 cctggtgacg caaaatattg cccagcattc tgccctacaa ctgacagagg ccgcgcgccc
     4261 ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc
     4321 gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg
     4381 caaactgcgt aaatcgattg ccgatgaaag taatgtcccg ccgtacgtgg tgtttaacga
     4441 cgcaaccttg attgagatgg ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt
     4501 taacggcgtt gggatgcgca agctggaacg ctttggtaaa ccgtttatgg cgctgattcg
     4561 tgcgcatgtc gatggcgacg acgaagagta gtcagcagca taaaaaagtg ccaggatgaa
     4621 gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg atgttatttc tcaccgtcgc
     4681 catggtgcac attgtggcgc ttatgagtcc cggtcccgat ttcttttttg tctctcagac
     4741 cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg ctgggtatta cctgcggcgt
     4801 tatggtttgg gcagggattg cgttgcttgg cctgcatttg attatcgaaa aaatggcctg
     4861 gctgcatacg ctgattatgg ttggcggtgg cctgtatctc tgctggatgg gttaccagat
     4921 gctgcgcggc gcgctgaaaa aagaggcggt ttctgcacct gcgccacagg tcgagctggc
     4981 gaaaagtggg cgcagtttcc tgaaaggttt actgaccaat ctcgctaatc cgaaagcgat
     5041 tatctacttt ggctcggtgt tctcattgtt tgtcggtgat aacgttggca ctaccgcgcg
     5101 ctggggcatt tttgcgctga tcattgtcga aacactggcg tggtttaccg tcgttgccag
     5161 cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt ctggcgaagt ggattgatgg
     5221 ttttgccggg gcgttatttg ccggatttgg cattcatttg attatttcgc ggtgatgcca
     5281 gacgcgtctt cagagtaagt cggataaggc gtttacgccg catccgacat tatttttcac
     5341 gcatgcctcg ccgatgctaa cagtgctccc accagcataa acaacgagcc gaaaatctta
     5401 ttcagcgcct tcatctgctt tggtccttta atccatagag caatccgttg agcaagggtg
     5461 gcgtaaccga tcatcacaat aatatcgacc acaatagtgg tgacgccgag cacgatatac
     5521 tgcatcagtt gcggctgttg cggcatgatg aattgcggaa atagcgccgc cagaaacaca
     5581 atacttttgg gattggtgag attcacaaaa actgcgcgct ggaacaaatg tcgacgcgat
     5641 tgagtagagg ccagcgattt aagatcaatt gcgccagcgg cgcgccattg ctgtattccc
     5701 agccaaatca agtaagccgc gcctgcccac ttcaacactt caaacgcaat cactgagcgg
     5761 gaaaatagcg tccccaaccc cacgccaacc agcacaatat gaatcgccag tccggtctga
     5821 agcccagcaa tagacgccac cgcgccgcga taaccgtggt tgagcgaggt ggtcatagtg
     5881 ttgattgcac cagagcctgg cgacaggctt aaaatgatcg atgtcagcag gtaggcaaac
     5941 caccattcta aggtcatgat gaactcccgg tgtgtctatt tttgtgccac aatacgctac
     6001 tgtcgcagcg ttgtgtcagg cacgctaaaa aaaacgattt tacgtggttt aagaggcaga
     6061 ttacccgatg tttcagcagc aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt
     6121 taccatggga ccgctgactg atttctggcg tcagcgtgat gaagcagagt ttactggtgt
     6181 ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag caccatgacc gggtggtagt
     6241 catctgcccg gggcgtattg agagctacgt aaaatatgcg gaactggcct acgatttgtt
     6301 ccatttgggg tttgatgtct taatcatcga ccatcgcggg cagggacgtt ccggtcgcct
     6361 gttagccgat ccgcatctcg ggcatgttaa tcgctttaat gattatgttg atgatctggc
     6421 ggcattctgg caacaggagg tccagcccgg tccgtggcgt aaacgctaca tactggcaca
     6481 ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc catccaggtg tatgtgacgc
     6541 cattgcgcta actgcgccaa tgtttgggat cgtgattcgt atgccgtcat ttatggcacg
     6601 gcagatcctc aactgggccg aagcgcatcc acgtttccgt gatggctatg caataggcac
     6661 cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg acccacagca gacagcgata
     6721 tcgacgtaac ttacgcttct atgctgatga cccaacgatt cgcgtcggtg ggccgaccta
     6781 ccattgggta cgcgaaagta ttctggctgg cgaacaggtg ttagccggtg cgggtgatga
     6841 cgccacgcca acgcttctct tgcaggctga agaggaacgc gtggtggata accgcatgca
     6901 tgaccgtttt tgtgaactcc gcaccgccgc gggccatcct gtcgaaggag gacggccgtt
     6961 ggtaattaaa ggtgcttacc atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc
     7021 gctccacgcc atcgttgatt ttttcaacag gcataactca cccagcggaa accgctctac
     7081 agaggtttaa atttcttatg taccaggttg ttgcgtctga tttagatggc acgttacttt
     7141 ctcccgacca tacgttatcc ccttacgcca aagaaactct gaagctgctc accgcgcgcg
     7201 gcatcaactt tgtgtttgcg accggtcgtc accacgttga tgtggggcaa attcgcgata
     7261 atctggagat taagtcttac atgattacct ccaatggtgc gcgcgttcac gatctggatg
     7321 gtaatctgat ttttgctcat aacctggatc gcgacattgc cagcgatctg tttggcgtag
     7381 tcaacgacaa tccggacatc attactaacg tttatcgcga cgacgaatgg tttatgaatc
     7441 gccatcgccc ggaagagatg cgctttttta aagaagcggt gttcaaatat gcgctgtatg
     7501 agcctggatt actggagccg gaaggcgtca gcaaagtgtt ctttacctgc gattcccatg
     7561 aacaactgct gccgctggag caggcgatta acgctcgctg gggcgatcgc gtcaacgtca
     7621 gtttctctac cttaacttgt ctggaagtga tggcgggcgg cgtgtcgaaa ggccatgcgc
     7681 tggaagcggt ggcgaagaaa ctgggctaca gcctgaagga ttgtattgcg tttggtgacg
     7741 ggatgaacga cgccgaaatg ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc
     7801 accagcgtct gaaagatctg cattcggagc tggaagtgat tggtactaat gccgaagacg
     7861 cggtaccgca ttatctgcgt aaactctatt tatcgtaatc gttctttatt tggtcagttg
     7921 tcaacctgat acttcgctac aatggatact cgttaatcaa agagttttcc attgtggcgc
     7981 tacttatcat caccacgatt ctgtgggcct tctcctttag cttttatggc gagtaccttg
     8041 cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt
     8101 ttctgccgtt tctgcgtacc cgtggcaata gcctgaaaac agtcggcctg tatatgctgg
     8161 tgggcgcgat gcagctcggc gtgatgtata tgctgagttt ccgcgcttat ctctacctga
     8221 cggtttccga actgctgctg ttcaccgtac tgacgccgct ctacatcacg ctgatttatg
     8281 acatcatgag taagcgccgt ctgcgctggg gatatgcttt tagtgccttg ttggcggtga
     8341 ttggtgccgg gattattcgc tatgatcagg tcaccgacca tttctggact ggcttgttgc
     8401 tggtgcaact ctccaatatc acttttgcca tcggcatggt gggttacaaa cgcctgatgg
     8461 aaactcgccc gatgccgcag cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg
     8521 tggcggtaat tgcatgtttc ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc
     8581 aatggggcat tctggtgttt cttggcgtgg tggcttccgg gattggctac tttatgtgga
     8641 actacggcgc aacccaagta gacgccggaa cgctgggcat tatgaataat atgcacgttc
     8701 cggcagggct gctggttaac ctggctatct ggcaccaaca gccgcactgg ccaacgttta
     8761 ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc
     8821 gctcttcaca aacggcagat gatcgcaggc gtgattgcgc gctgagcgaa taaatgcttc
     8881 tgtaatcggc tgacgctgct cgccatcgcg cacggcggcg tacagccggc tccacaagcc
     8941 ttcgcccagg gttttggtcg ccaccagatc ctggcgctca aaactctcta ctacccaatg
     9001 tggtagcgcg gcaataccca tccgcgcggc aaccatctga atcaacaata aggtgttatc
     9061 gatgcttttc agtgacgggc tgacgcctgc cggctgaaga aaatgccgcc agacatccag
     9121 tcgactacgc tgcaccggat aaattaatag cgtctcgctg gcgagatctt ccggtgtaat
     9181 acgcgttttt gccgccagtg gatggtcagg tgctaacacc aggcgtactt cataatcgaa
     9241 catcggcgaa taatgcaggc cactgcgcgg caggatatcg gaggtcatca ccagatccag
     9301 ctcgccctgt tgcaacgcgg gctgcgggtc aaacgtcacg cccgatttaa aatccatctc
     9361 tacctgcggc cagttcttat ggaaattttc taacgcgggt gtcagccact gaatacagct
     9421 atggcactca atggcaatgc gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc
     9481 ctggctgatt tgcggcagca cctggtttgc cagttgcaac aggatttctc cctgcggtgt
     9541 aaagcgtagc ggctggctct tacgcacaaa tagccggaag ccaaggcgtt gttccagatc
     9601 gctaaactgg tgagacaggg cggattgcgt ctgatgcaac gtcgccgcag cggctgcgag
     9661 agagccgcag ttccgcaacg cttgtagcgt tttcaggtgt tttacttcga tcatgaaagt
     9721 ccttcacttc ggcatgaata atttgcgctt gaggaatata cagtaaccgc caattatgga
     9781 tgtgtaaaca tctggacggc taaaatcatt cgtcttttaa atttatggtg cgttggctgc
     9841 gtttctccac cccggtcact tacttcagta agctcccggg gatgaataaa cttgccgcct
     9901 tccctaaatt caaaatccat aggatttaca tataattaga ggaagaaaaa tgacaatatt
     9961 gaatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga
    10021 gagttattgg gcggggaact ccacgcgtga agaactgctg gcggtagggc gtgaattgcg
    10081 tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt
    10141 tgcctggtac gatcatgtac tgaccaccag tctgctgctg ggtaacgttc cagcgcgtca
    10201 tcagaacaaa gatggttcgg tagatatcga caccctgttc cgtattggtc gtggacgtgc
    10261 gccgactggc gaacctgcgg cggcagcgga aatgaccaaa tggtttaaca ccaactatca
    10321 ctacatggtg ccggaattcg ttaaaggcca acagttcaaa ctgacctgga cgcagctgct
    10381 ggaggaagtg gacgaggcgc tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc
    10441 ggttacctgg ctgtggctgg ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct
    10501 gaacgccatt ctgccggttt atcagcaagt gctggcagaa ctggcgaaac gcggcatcga
    10561 gtgggtacag attgatgaac ccgcgctggt actggaacta ccacaggcgt ggctggacgc
    10621 atacaaaccc gcttacgacg cgctccaggg acaggtgaaa ctgctgctga ccacctattt
    10681 tgaaggcgta acgccaaatc tcgacacgat tactgcgctg cctgttcagg ggctacatgt
    10741 ggaccttgta catggtaaag atgacgttgt tgaactgcac aagcgcctgc cttctgactg
    10801 gctgctgtct gcgggtctga tcaatggtcg taacgtctgg cgcgccgatc ttaccgagaa
    10861 atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc
    10921 gttgctgcac agccccatcg acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag
    10981 ctggtttgcc ttcgccctac aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa
    11041 cagtggtgac acggcagctc tggcagagtg gagcgcccca attcaggcgc gtcgtcactc
    11101 tactcgtgta cataatccgg cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag
    11161 ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc
    11221 agcgtggccg accaccacga ttggttcctt cccgcaaacc acggaaattc gtaccctgcg
    11281 tctggatttc aaaaagggca atctcgacgc caataactac cgcacgggca ttgcggaaca
    11341 tatcaggcag gccattgttg aacaggaacg tttgggactg gatgtgctgg tacatggcga
    11401 ggccgagcgt aatgacatgg tggaatactt tggcgagcac ctcgacgggt ttgtctttac
    11461 gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg aagccaccga ttgttattgg
    11521 tgacgttagt cgcccggcac cgattaccgt ggagtgggcg aagtatgcgc aatctctgac
    11581 tgataaaccg gtgaaaggga tgctgactgg cccggtgacc attctctgct ggtcgttccc
    11641 gcgtgaagat gtcagccgtg aaaccatcgc caaacaaatt gcgctggcgc tgcgtgatga
    11701 agtggcggac ctggaagccg caggaattgg catcatccag attgatgaac cggcgctgcg
    11761 cgaaggttta ccgctgcgtc gtagcgactg ggatgcatat ctccagtggg gcgtggaggc
    11821 cttccgtatc aacgccgccg tggcgaaaga tgacacacaa atccacactc acatgtgtta
    11881 ttgcgagttc aacgacatca tggattcgat tgcggcactg gacgcagacg tcatcaccat
    11941 cgaaacctcg cgttctgaca tggagttgct ggagtcgttc gaagagtttg attatccgaa
    12001 tgaaatcggt ccaggcgtct atgacattca ctcgccaaac gtaccgagcg tggaatggat
    12061 tgaagccttg ctgaagaaag cggcaaaacg cattccggca gagcgcctgt gggtcaaccc
    12121 ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc gcggcactgg cgaacatggt
    12181 gcaggcggcg cagaacttgc gtcggggatg atgaatatta acgagtcact gtaaattgca
    12241 gtacgatgaa agaggcaaaa ctgcctgata ccaggcctac ataatttatg caatttattg
    12301 aattttaggg ttttgttggc tggacaaggc gttcacgccg catccggcat gaacaacgcg
    12361 cactttgcca ataatctgat ggcttccatc accggaagcc attttgctat ctgcgcccgt
    12421 tcagatacag aaataaccga atcattttta cgcaaaatat aatcctgagc gatttgttca
    12481 gtaatcaacg ctacgttatt tattctcact tttcacgaaa ttgtatccct tttcagtaac
    12541 gtggtagtga ggcatagact tatgtccgct atcctcaata ataaccaaac cctcgttagc
    12601 taatttgtta acaatgttaa caacattacc gaatcctgga atttgcctta tggatagtgt
    12661 gcgatcgaga ttcatccagt tccagtttat atctttctca gccataactt tcagtacgtt
    12721 aatttcagtt tcagttatca tagtcaagtg catcctggtg gtataagtca ttaagttctc
    12781 ccttaagttt ataatcttct agagtagcag tatgcgtatg attccagagt gcatagcctg
    12841 cgtcaccatc aggttgtcct gtttcccaac caagattaca gtaacgctga tattcccgac
    12901 actcatgagt atagaagtta agatctactt gttctggggg gatttttcct ttcttaatgt
    12961 ctttcagtct attgatcatc acttcattta cagggtcatg accaaatctg gctatgtgtt
    13021 tttcaacaat agcgatacct tccctgttga ttgttacgcc atctgtcgtc agattttcta
    13081 ttggaccacc ggcaaggtcg ggattaaatg gtcttcctga tactgctcct ctggctccga
    13141 aatgctcaag tgattccaaa tcagggagtc tcattctccc cagaactgct ccagcaacta
    13201 ttgaagatga gtaacccttt gttatccata aattgatcgt aaccagcagg tgcatctccc
    13261 cccaacttgt ctgctgtttc cctgaatcct tcgatattcc cattgtatat gccaccagca
    13321 gcaagcagtc tgccaaccgc tttactgtta agtgttttga aaccattacc agctttagtt
    13381 tttggccgtg gtataaacag acggttgtag ttatcgggaa atgccaaata actaaattca
    13441 gtattagcaa tcagatgcct ggagagtata tattcataac tgacagtgat ggcttctgta
    13501 tccagattgt tctcagtaaa caggtgattg attgctgata agagcgcgcc gcggagttga
    13561 atgttgtaat atttctccca tcgaccaaat ctattgatcc gatagaagtc aaactccatg
    13621 aacaactgct cgttattgtt aatagcgtta ataagtaacg gggagctttt gtcaatcaat
    13681 ttacagaaac taaggccatg caattgtgac cctttgcctg tattcgtaat actattagag
    13741 agcgagaacg cgaagatttc atcttcatgg ccaatctgcc agcgattacc tattgatgcg
    13801 ctcgttccac aacgagagga aatacatcct tgttgttctc cggttatggt caag
//
LOCUS       DAZUGK010000116        13770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZUGK010000116 DAZUGK010000000
VERSION     DAZUGK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.117"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..154)
                     /gene="yojO"
                     /locus_tag="ACHY5W_003028"
     CDS             complement(<1..154)
                     /gene="yojO"
                     /locus_tag="ACHY5W_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFS4447892.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSL"
     gene            263..757
                     /gene="napF"
                     /locus_tag="ACHY5W_003029"
     CDS             263..757
                     /gene="napF"
                     /locus_tag="ACHY5W_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFS4447893.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            747..1010
                     /gene="napD"
                     /locus_tag="ACHY5W_003030"
     CDS             747..1010
                     /gene="napD"
                     /locus_tag="ACHY5W_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFS4447894.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1007..3493
                     /gene="napA"
                     /locus_tag="ACHY5W_003031"
     CDS             1007..3493
                     /gene="napA"
                     /locus_tag="ACHY5W_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFS4447895.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3500..4195
                     /gene="napG"
                     /locus_tag="ACHY5W_003032"
     CDS             3500..4195
                     /gene="napG"
                     /locus_tag="ACHY5W_003032"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFS4447896.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4182..5045
                     /gene="napH"
                     /locus_tag="ACHY5W_003033"
     CDS             4182..5045
                     /gene="napH"
                     /locus_tag="ACHY5W_003033"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFS4447897.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5042..5491
                     /gene="napB"
                     /locus_tag="ACHY5W_003034"
     CDS             5042..5491
                     /gene="napB"
                     /locus_tag="ACHY5W_003034"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFS4447898.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5501..6103
                     /gene="napC"
                     /locus_tag="ACHY5W_003035"
     CDS             5501..6103
                     /gene="napC"
                     /locus_tag="ACHY5W_003035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFS4447899.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6116..6739
                     /gene="ccmA"
                     /locus_tag="ACHY5W_003036"
     CDS             6116..6739
                     /gene="ccmA"
                     /locus_tag="ACHY5W_003036"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFS4447900.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6736..7398
                     /gene="ccmB"
                     /locus_tag="ACHY5W_003037"
     CDS             6736..7398
                     /gene="ccmB"
                     /locus_tag="ACHY5W_003037"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFS4447901.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7440..8177
                     /gene="ccmC"
                     /locus_tag="ACHY5W_003038"
     CDS             7440..8177
                     /gene="ccmC"
                     /locus_tag="ACHY5W_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFS4447902.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8174..8383
                     /gene="ccmD"
                     /locus_tag="ACHY5W_003039"
     CDS             8174..8383
                     /gene="ccmD"
                     /locus_tag="ACHY5W_003039"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFS4447903.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8380..8859
                     /gene="ccmE"
                     /locus_tag="ACHY5W_003040"
     CDS             8380..8859
                     /gene="ccmE"
                     /locus_tag="ACHY5W_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFS4447904.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8856..10799
                     /gene="ccmF"
                     /locus_tag="ACHY5W_003041"
     CDS             8856..10799
                     /gene="ccmF"
                     /locus_tag="ACHY5W_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFS4447905.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRMAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVASMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGSLVRWGRDRPRKIRNLLIFAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10796..11353
                     /gene="dsbE"
                     /locus_tag="ACHY5W_003042"
     CDS             10796..11353
                     /gene="dsbE"
                     /locus_tag="ACHY5W_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFS4447906.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11350..12402
                     /gene="ccmH"
                     /locus_tag="ACHY5W_003043"
     CDS             11350..12402
                     /gene="ccmH"
                     /locus_tag="ACHY5W_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFS4447907.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12613..13260)
                     /gene="narP"
                     /locus_tag="ACHY5W_003044"
     CDS             complement(12613..13260)
                     /gene="narP"
                     /locus_tag="ACHY5W_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFS4447908.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13580..>13770
                     /locus_tag="ACHY5W_003045"
     CDS             13580..>13770
                     /locus_tag="ACHY5W_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447909.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINSGG"
BASE COUNT         2927 a         3479 c         4040 g         3324 t
ORIGIN      
        1 tgagagagca aaccagcaga aatatctctg gtgaattaat cgctatataa atatatttat
       61 aaccatttga aatgtgagca aaagcccgtt tttgccacgc ttcccgccac tcttttgatc
      121 ctgctacagg ttttacctcg atcggggtat gcatctttga cacatccttt aatatcttag
      181 cggctataaa aatggcttat taattatgcg gtttatttgg tcgctctcaa ttttcagagc
      241 gcgttaatga tggaaggtca atgtgaagat tgatgcatcc cgtcggggca tactcactgg
      301 tcgctggcgc aaagccagta acggtatccg tccgccctgg tccggtgatg aatctcattt
      361 tctgacccat tgcacccgct gcgacgcctg tattaatgct tgtgaaaaca acattctgca
      421 acgcggcgcg ggcggctatc cgagcgttaa tttcaaaaac aacgaatgca gcttctgtta
      481 cgcctgcgcg caggcttgcc ccgaatcgct attttctccg cgccacacca gggcctggga
      541 tttgcagttc accattgggg acgcgtgcct ggcgtatcag tcagttgaat gccgccgctg
      601 tcaggatagc tgtgagccaa tggcaattat ctttcgcccg acgctctccg ggatctacca
      661 gccgcaactt aatagccaac tttgcaacgg atgtggagca tgtgcagcca gctgtccggt
      721 atcagccata actgcggagt atctccatgc acactaactg gcaagtttgc agcctggtcg
      781 tgcaggccaa aagcgaacga atttcagaca tcagcaccca actgaacgcc tttcccggct
      841 gtgaagttgc tgtcagcgac gcgccgagcg gtcagttgat tgtggtggtg gaagcagaag
      901 acagcgaaac gctgatccaa accattgagt cagtacgcaa cgtagagggc gtgctggcgg
      961 tgtcgctggt ttatcaccag caggaagagc aaggtgagga aacaccatga aactcagtcg
     1021 tcgtagcttt atgaaagcta acgccgttgc ggccgctgcg gcggctgccg gtctcagcgt
     1081 gccgggcgtt gcccgcgccg ttgttggtca gcaggaagcc atcaaatggg ataaagcgcc
     1141 gtgccgtttc tgcggtactg gttgcggcgt tctggtcgga acgcagcagg ggcgtgtggt
     1201 ggcctgtcag ggcgacccgg acgcaccggt taaccgtggc ctgaactgca ttaagggcta
     1261 tttcctgccc aagatcatgt acggtaaaga ccgtttgacg cagccgctgc tgcgtatgaa
     1321 aaacggtaaa tatgacaaag aaggcgaatt taccccaatc acctgggatc aggccttcga
     1381 tgtgatggaa gagaaattca aaaccgccct gaaagaaaaa gggccggaat cgatcggtat
     1441 gttcggttct ggtcagtgga ctatctggga aggttatgcc gcgtccaagc tgtttaaagc
     1501 gggcttccgt tcgaacaaca tcgacccgaa cgcgcgtcac tgtatggcgt cggcagtagt
     1561 tggctttatg cgtacctttg gtatggatga gccgatgggc tgctatgacg acattgagca
     1621 ggctgacgcg tttgtgctgt ggggcgcaaa catggcggag atgcacccga tcctctggtc
     1681 acgcatcact aaccgtcgtc tctctaacca ggacgtcacc gtggcggtgc tttcaaccta
     1741 ccagcatcgt agcttcgagc tggcggataa cggcatcatc tttacgccgc aatctgacct
     1801 ggtgatcctg aactacatcg ccaactatat cattcaaaac aatgcgataa atcaggactt
     1861 cttcagcaag cacgttaacc tgcgcaaagg ggcgacggac atcggctacg gtttacgtcc
     1921 gacccatccg ctggaaaaag cagcgaagaa tccgggttct gacgcctccg aaccgatgag
     1981 cttcgaagat tacaaagcct tcgttgctga gtatacgctg gaaaaaaccg ctgaaatgac
     2041 cggcgtgccg aaagaccagt tagaacaact ggcgcagctg tatgccgatc cgaacaagaa
     2101 agtcatctcc tactggacga tgggcttcaa ccagcatact cgtggcgtgt gggccaacaa
     2161 cctggtctac aacctgcacc tgctgaccgg caaaatttcc cagccgggtt gcggtccgtt
     2221 ctccctgacc gggcagcctt ccgcgtgtgg tactgcgcgt gaagtgggca cctttgccca
     2281 ccgtctgcct gcggacatgg tggtgactaa cgagaaacat cgtgatatct gcgagaagaa
     2341 gtggaatatc ccgagcggca ccattccggc gaaaatcggt ctgcatgcgg tagcacaaga
     2401 ccgtgcgctg aaagacggca agctgaatgt ttactggacc atgtgtacca acaacatgca
     2461 ggccgggccg aacatcaatg aagagcgtat gccgggctgg cgcgatccgc gcaacttcat
     2521 catcgtctcc gatccgtatc cgacagtcag tgcgctggcc gccgacttga tcctgccgac
     2581 cgcaatgtgg gtagaaaaag agggcgctta cggtaacgcc gaacgccgta ctcagttctg
     2641 gcgtcagcaa gtacaggcac ctggtgaagc gaaatcggat ctctggcagt tagttcagtt
     2701 ctcccgtcgc ttcaaaaccg aagaagtatg gccggaagag ctgctggcga agaaaccgga
     2761 actgcgtggc aaaacgctgt acgaagttct gtatgccacc cccgaagtga gcaaattccc
     2821 ggtatccgaa ctggcggaag atcagctgaa cgatgaatcc cgcgagctgg gcttctatct
     2881 gcaaaaaggg ctgttcgaag agtacgcatg gtttggtcgc ggtcacggtc acgatctcgc
     2941 gccgttcgat gactaccaca aagcgcgcgg tctgcgctgg ccggtggtga acggtaaaga
     3001 aacgcagtgg cgttacagcg aaggtaacga cccgtacgtg aaagcgggcg aaggctataa
     3061 gttctacggt aaaccggatg gtaaagcggt gatcttcgcg ctgccgttcg aaccggcggc
     3121 ggaagcaccg gatgaagagt acgacctgtg gctctctacc ggacgcgtcc tggagcactg
     3181 gcacaccggc agtatgactc gccgtgtgcc ggaactgcac cgcgccttcc cggaagcggt
     3241 cctgtttatt cacccgctgg atgcgaaagc gcgcgatctg cgccgtggcg acaaagtgaa
     3301 agtggtttct cgccgtggcg aagtcatctc gattgttgaa acgcgcggtc gtaaccgtcc
     3361 gccacagggc ctggtgtaca tgccgttctt cgacgccgca cagctggtta acaaactgac
     3421 gctggacgcg accgatccgc tctcgaaaga gacggacttc aagaagtgcg cggtcaaact
     3481 ggagaaggtg taagccatta tgtcccggtc agcgaaacct caaaatggtc gccgccgctt
     3541 tctgcgcgat gttgttcgca cagcaggcgg gctggctgcc gtgggtgtgg cgctggggtt
     3601 acaacagcaa accgcacgcg catctggcgt gcggttgcgc ccgcccggag ccataaacga
     3661 gaacgccttt gccagtgcct gtgtgcgttg tggtcagtgt gttcaggctt gcccttacga
     3721 caccttaaaa ctggcgacgc tggcctctgg tctgtcggcg ggcacgccat attttgtcgc
     3781 acgggatatt ccttgcgaaa tgtgtgagga cattccgtgc gccaaagtgt gcccgagcgg
     3841 tgcgctggat cgtgagattg aatcgatcga cgacgcgcgg atggggctgg cggtactggt
     3901 ggaccaggaa aactgtctca actttcaggg gctgcgctgc gatgtttgtt atcgcgaatg
     3961 cccgaaaatt gatgaggcca tcaccctgga gctggagcgc aacacgcgta ccggtaagca
     4021 cgcccgcttt ctgccgacgg ttcacagcga cgcctgtact ggttgcggta agtgcgaaaa
     4081 agtgtgcgtg ctggaacaac cggcaatcaa ggtgttaccg ctgtcactgg cgaaagggga
     4141 gttaggtcac cattaccgct tcggctggct ggaggggaac aatggcaaat cgtaaacgtg
     4201 acgccgggcg cgaggcgctg gagaaaaaag gctggtggcg cagtcaccgt tggctggtgt
     4261 tacgtcgcct ttgtcagttc ttcgtgctgg ggatgttttt gagcggtccg tggtttggtg
     4321 tgtggatctt gcacggcaac tacagcagta gcctgttatt cgacaccgtt ccgctgaccg
     4381 atccgctgat gacgctgcaa agtcttgcca gtggtcatct gcctgccacg gtagcgctga
     4441 ctggtgcggt gattatcacc gtgctctacg ccctggcagg aaagcgatta ttttgcagct
     4501 gggtctgccc gctgaacccg attaccgact tagcgaactg gttacgcagg cgctttgacc
     4561 tcaatcagtc tgcgacgatc cctcgccaca ttcgctacgt gctgttggtg gtcattctgg
     4621 tgggatctgc cttaactggc acgctcatct gggaatggat caaccccgtt tccctgatgg
     4681 ggcgtagcct ggtaatgggc ttcggcagcg gcgcgctgct tattctcgcg ctgtttttat
     4741 ttgatctact ggtcgttgaa cacggctggt gcgggcacat ttgtccggtt ggcgcgctgt
     4801 atggcgtgct gggtagcaaa ggtgtgatta ccgttgcggc cagcgatcgg cagaaatgta
     4861 accgctgtat ggattgtttt catgtttgcc cggaaccgca tgtgctacgt gccccggtgc
     4921 tggatgaaca aagcccggtg caggtcacca gccgcgattg catgacgtgc ggtcgctgcg
     4981 tggatgtctg ttctgaggat gtatttacaa taactacacg atggagttcg ggagcgaaat
     5041 catgaaaagc catgacctga agaaagcgct gtgtcaatgg acggcgatgc tggccctggt
     5101 ggtaagcggc gcggtttggg cggctaatgg cgtcgatttt agccagtcgc cggaagtatc
     5161 cgggacgcag gaaggggcca ttcggatgcc aaaagagcag gatcggatgc cgctgaacta
     5221 tgtgaatcag ccgccaatga tcccgcatag cgttgaaggt tatcaggtaa cgaccaatac
     5281 caaccgctgc ttgcagtgcc acggtgtcga aagctatcgc accactggcg cgccgcgtat
     5341 cagtcctact cactttatgg acagcgacgg caaagtgggc gcggaagtgg ctccgcgtcg
     5401 ttatttctgt ctgcaatgtc acgtaccgca ggccgatacc gcgccaatcg tggggaatac
     5461 ctttacccca tcaaaaggtt acgggaaata agaggttatt atgggaaatt ctgaccgtaa
     5521 gcctggtctg attaagcgcc tgtggaaatg gtggcgtacc cccagccgtc tggcgctggg
     5581 gacgctgctg ttgatcggtt ttgtcggcgg catcgtcttc tggggcggct ttaacactgg
     5641 gatggaaaaa gccaataccg aagagttctg cattagctgc cacgaaatgc gtaacacggt
     5701 gtatcaggaa tacatggatt ccgtgcacta caacaaccgt agcggcgtcc gtgcgacctg
     5761 tccggattgt cacgttccgc acgagtttgt gccgaagatg atacgcaagc tcaaagcaag
     5821 taaagagctg tatggtaaaa tttttggcgt tattgacacg ccgcagaaat ttgaagctca
     5881 tcgtctgacg atggcacaga atgagtggcg gcgcatgaag gacaataact cgcaggagtg
     5941 ccgtaactgt cacaacttcg agtatatgga tacaaccgcc cagaaatcgg ttgccgcgaa
     6001 gatgcatgac caggcggtga aagatgggca aacctgtatt gattgccata aagggatagc
     6061 gcacaagctg cccgatatgc gtgaagtcga gccaggtttt taacagggtt attgcgtggg
     6121 tatgcttgaa gccagagagt tactttgtga gcgggatgaa cgaaccttat ttagtggctt
     6181 gtcatttacg ctgaacgcag gagagtgggt acaaatcacc ggtagcaacg gcgcggggaa
     6241 gacaacgctt ctccgtttgc tgacggggtt gtctcgccct gacgcaggcg aggttctctg
     6301 gcaagggcag cccttgcatc aggtacgcga cagctaccat caaaacctgt tatggatagg
     6361 ccatcagccg gggatcaaaa cccggctgac ggcgttagaa aatctgcact tttatcatcg
     6421 cgatggcgat accgcacaat gtctggaagc cctggcgcag gccgggcttg ccggattcga
     6481 agatattccg gtaaatcaac tttctgccgg gcaacaacgc cgcgtcgctt tagcgcgtct
     6541 gtggctgacc cgtgccacgt tatggatcct cgacgagcct tttaccgcga ttgacgtcaa
     6601 cggcgtcgat cgtctgaccc agcgtatggc gcagcatacg gagcaggggg ggattgtgat
     6661 tctgactacc caccagccgc tcaacgttgc tgaaagtaaa attcgccgca tttcactgac
     6721 gcaaacgagg gccgcatgat gttctggcgc attttccgtc ttgagctgcg cgtagcgttt
     6781 cgccatagcg ccgaaatcgc caacccgctg tggttcttcc tgattgtaat tacccttttt
     6841 ccgctcagta tcggtccgga gccgcaactg ctggcgcgta ttgcaccggg cattatctgg
     6901 gttgctgcgc tgctttcatc cttgctggcg ctggaacgac tgttccgtga cgatttgcag
     6961 gacggcagtc ttgaacaatt gatgttgttg ccgttaccct tgcccgccgt tgtgctggcg
     7021 aaggtgatgg cgcactggat ggtaaccggt ctgccgttac tcatcctttc gcccctggta
     7081 gcaatgctac tgggaatgga tgtttatggc tggcaagtga tggcgttaac gctactgctg
     7141 ggaaccccta cgcttggctt tctcggtgca ccgggcgtgg cgctgacagt gggacttaag
     7201 cgcggtggtg tgctgctcag catactggtg ttaccgctga ctatcccatt actcatcttt
     7261 gccaccgccg cgatggacgc ggcttccatg catttacctg tagatgggta tctggcaatt
     7321 ttaggcgcgt tgctggcagg caccgcgaca ttaagtcctt ttgcgacggc ggcagcgtta
     7381 cgaatcagca ttcaataacg ctggattact gatttttgtt cgagtctggt atcgaaacta
     7441 tgtggaaaac actgcatcaa ctggcgatcc caccacggct gtatcaaatc tgtggctggt
     7501 ttataccgtg gctggcaatt gccagtgtgg tcgtgcttac cgtcggctgg atctggggat
     7561 tcggctttgc tccggctgat tatcagcagg gaaatagcta ccgcattatc tacctgcatg
     7621 tgcctgcggc gatctggtcg atgggcattt atgcatcaat ggcggtggca gcgtttattg
     7681 gccttgtctg gcagatgaaa atggccaacc tggcggtggc ggcgatggcc cccattggtg
     7741 ccgtgtttac ctttattgcc ctggttaccg gctctgcatg gggaaaaccg atgtggggca
     7801 cctggtgggt atgggatgca cgtctgactt ctgaactggt gctgctgttt ttgtatgtgg
     7861 gtgtcattgc cctgtggcac gccttcgacg accgccgtct ggcgggccgt gcggcaggta
     7921 tcctggtgct gattggcgtg gtgaatctgc cgattattca ttactccgtg gagtggtgga
     7981 acaccctgca tcagggatca acgcggatgc agcaaagtat cgatccggcg atgcgttcgc
     8041 cgctgcgctg gtcgattttt ggcttcctgc tcctgtctgc cacgctgacg ctgatgcgga
     8101 tgcggaattt gattttgctg atggaaaaac gccgtccgtg ggtgagtgaa ctgatactga
     8161 aaagaggccg taaatgaccc ctgcatttgc ttcctggaat gaatttttcg caatgggcgg
     8221 ttacgccttt tttgtctggc tggcggtggt gatgaccgtt attccgctgg tggttttggt
     8281 cgtgcactcg gtgatgcaac atcgcgcaat tctgcgtggt gtggcgcaac agcgggcgcg
     8341 tgaggcgcgt ttacgtgctg cgcaacagca ggaggctgca tgaatattcg ccgtaaaaac
     8401 cgcttgtgga ttgcctgtgc cgtgttggca gggctggcgc tgactatcgg tctggtgcta
     8461 tatgcgctgc gctcgaatat cgatctcttt tatacgccgg gggaaattct ctacggcaag
     8521 cgtgaaactc agcaaatgcc ggaagtcggt cagcgtctgc gcgttggcgg gatggtgatg
     8581 ccgggtagtg tgcagcgcga tcccaattcg ctgaaagtga ccttcaccat ttacgacgct
     8641 gaaggctcag tggatgtctc ttacgaaggc attttgccgg atctgttccg tgaagggcag
     8701 ggcgttgtgg tgcagggcga gctggaaaaa ggcaatcata tcctcgcgaa agaagtgctg
     8761 gcgaaacacg acgaaaacta cacgccgcca gaagttgaga aagcgatgga agctaaccac
     8821 cgtcgcccgg cgagtgttta taaggaccca gcatcatgat gccggaaatt ggaaacgggc
     8881 tgctgtgcct ggcgttaggg attgcgctgc tgctgtccgt gtatccgcta tggggcgtgg
     8941 cgcgcggaga tgcgcgtatg atggcgtctt cccgcttgtt tgcctggctg ctgtttatgt
     9001 ctgtggctgg cgcatttctg gtgctggtca atgctttcgt ggtcaacgac tttaccgtca
     9061 cctatgttgc cagcaactcc aatacccagc ttccggtgtg gtatcgcatg gcggctacct
     9121 ggggcgcaca tgaaggctca ctgctgctgt gggtgctgct gatgagcggc tggacctttg
     9181 cggtggcgat ttttagtcag cgtattccgc tggatattgt ggcccgcgta ctggcgataa
     9241 tggggatggt cagcgtcggc tttttgctgt tcattctctt tacctctaac ccattctcac
     9301 gcacgctgcc gaacttcccg attgaagggc gcgatcttaa cccgctgtta caggatccgg
     9361 ggctgatctt ccatccgcct ctgctctata tggggtacgt gggtttctcg gtggcgtttg
     9421 cttttgccat tgcttctttg ctgagcgggc gtctggacag cacttatgcg cgttttactc
     9481 gtccgtggac actggcagcg tggatcttcc tgacgctcgg catcgtgctc ggttccgcat
     9541 gggcctatta cgaactcggc tggggtggct ggtggttctg ggatccggta gaaaacgcct
     9601 cgtttatgcc gtggctggtg gggactgcgc tgatgcactc actggcggtc actgaacaac
     9661 gcgccagctt caaagcgtgg acattactgc tggcaatcag tgccttctcg ttgtgtctgt
     9721 tggggacttt cctcgtgcgt tccggcgtgc tggtatcggt acacgcgttt gcgtctgatc
     9781 cggcgcgcgg tatgtttatc ctcgccttta tggtgctggt gattggcggt tcgctgctgc
     9841 tgtttgccgc gcgtggacac aaagttcgct cacgcgtaaa caatgcgctg tggtcgcggg
     9901 aatctttgct gttagcgaac aacgttttgc tggtcgcctc gatgctggtg gtgttgctgg
     9961 ggacgctgct gccgctggtg cacaagcaac tgggactggg cagtatttcg attggcgaac
    10021 cgttcttcaa caccatgttt acgtggctga tggtgccgtt tgcgctactg cttggtgtcg
    10081 gttctctggt gcgctggggg cgcgatcgcc cacgtaagat ccgcaattta ttgattttcg
    10141 cctttatctc tacgctggtg ctgtctctgc tgttgccgtg gctgttcgaa agcaaagtgg
    10201 tggcgatgac ggtgctcggc ctggcaatgg cctgctggat tgcggtgctg gcaattgcgg
    10261 aagctgcgct acgtatttca cgcggcacga aaaccacctt cagttattgg gggatggtgg
    10321 cggctcacct ggggctggca gtgacaattg ttggcattgc ctttagccag aattatagcg
    10381 ttgagcgtga tgtgcgcatg aagtccggcg atagcgtcga tattcatgaa tatcgcttca
    10441 ccttccgtga cgtcaaagag gtgactggcc cgaactggcg cggcggtgtg gcgactatcg
    10501 gcgtaacgcg agagggtaaa ccggaaacgg tgctgtatgc ggaaaaacgt tattacaaca
    10561 ctgccgggtc gatgatgacc gaagcggcaa ttgacggcgg catcacgcgt gacctgtacg
    10621 ccgcgctcgg tgaagagctg gaaaacggcg cgtgggctgt gcgtctttac tacaaaccat
    10681 ttgttcgctg gatttgggcg ggcgggctga tgatggcgtt gggcggactg ctgtgtctgt
    10741 ttgatcctcg ctatcgtaag cgcgtgagcc cgcaaaaaac tgcgccggag gctgtatgaa
    10801 gcgcaaagta ttgttaattc cgttgattat cttcctggcg attgccgcgg cgctgctgtg
    10861 gcagctggcg cgtaatgccg aaggggatga tccgaccaat ctggaatcgg cgctcattgg
    10921 caagccggtg ccgaagtttc gtctcgaatc actggacaat ccggggcagt tttatcaggc
    10981 ggatgtgctg actcagggca aaccggtgct gcttaacgtc tgggcgacct ggtgtccgac
    11041 ctgccgtgcg gaacatcaat atctgaatca gctttctgcg cagggtatcc gcgtggttgg
    11101 catgaactat aaagacgatc gccagaaggc aatcagctgg ctgaaagagc tgggcaatcc
    11161 ttacgcgcta agcctgtttg atggcgacgg catgttaggg ctggatctcg gtgtctatgg
    11221 cgcgccagaa acgttcctta ttgacggcaa cggcatcatt cgctatcgcc atgcgggcga
    11281 tctgaatcct cgcgtctggg aagaagagat caagccgctg tgggagaaat acagtaagga
    11341 ggccgcacaa tgaggttttt attgggcgtg ctgatgctga tgatctccgg ctcagcgctg
    11401 gcgaccatcg atgtgttgca gtttaaagat gaagcacagg aacaacagtt ccgtcagctc
    11461 actgaagaac tgcgttgccc gaaatgccag aacaacagca ttgccgattc caactcgatg
    11521 attgccaccg acctgcgcca gaaagtgtat gaactgatgc aggaaggtaa aagtaagaaa
    11581 gagattgtcg attatatggt ggcgcgttac ggcaacttcg tcacttacga tccgccgtta
    11641 acgccgctga ccgtgctgct gtgggtgctg ccagtagtgg ctattggcat tggcggttgg
    11701 gtcatatacg cccgctcgcg gcgtcgggta cgcgtggtgc tggaagcgtt tcctgaacaa
    11761 agcgtgccgg aaggtaagcg tgccggatat gttgtttatc tgccgggtat tgtggtggcg
    11821 ttaattgtgg ctggcgtcag ctactaccag actggcaatt atcagcaggt gaaaatctgg
    11881 cagcaggcca cggcacaggc tccggcgttg ctggacaggg cgctggatcc gaaagccgat
    11941 ccgctcaacg aagaagagat gtcgcgcctt gcgctgggga tgcgtactca actgcaaaaa
    12001 aatccgggag atatagaagg ttggattatg ttgggtcgcg ttggcatggc gctgggtaac
    12061 gccagtatcg ccaccgatgc atacgctact gcgtatcgcc tcgatccgaa aaacagcgat
    12121 gccgcactgg gttatgctga agcgttgaca cgttcatctg atcccaacga caaccgcctc
    12181 ggcggcgaac tgctgcgcca gttggtgaga acggaccata gcaatattcg tgtgttaagc
    12241 atgtatgcgt ttaatgcctt tgagcagcag cgatttggcg aagccgttgc cgcgtgggag
    12301 atgatgttga aactcttacc tgccaacgat actcgccgtg ctgtgattga acgtagtatc
    12361 gcgcaggcga tgcaacattt gtcgccgcag gagagtaaat aaaaagtggc ccgatggtga
    12421 ctccatcagg ccagactgat gacaaagtgc gcttagttta tgccggatgc ggcgtgaacg
    12481 ccttatccgg cctacacaat cgtgtgaatt caataaattg atcgatccgc gtaggcctga
    12541 taagcgtagc gcatcaggca gttttgcgtt tgtcataaat ctcagcccga tgataaatcc
    12601 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg
    12661 tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg tctgctctga
    12721 aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    12781 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    12841 ttcatgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccggcacg
    12901 aatcgcttcc agcaacactt ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc
    12961 gtctatcagt gcaaagacat cgctggaggc atctgatacg gtcaggataa taatttgcgc
    13021 ggtaacgcca tccctgcgca aggcattgag ggtatctagg ccactcatac ctttcatatt
    13081 gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatag cactcgcgcc
    13141 gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact gacgaacacc
    13201 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    13261 agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    13321 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttgt
    13381 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct tctcatcaag
    13441 aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt ttttaaaaac
    13501 tgactccggg tatggagcta tgggtatttt ctctacccaa tgcttttaac agcaattaat
    13561 ttcataggat gaaagctcaa tgcatcaatc tggttctgtt tctctttgtc gttccgcaat
    13621 atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa cagttgagta
    13681 tggtgagaca gttgatggtg ttgtcctgga aaaagatatc cagctggttt atgggaccgc
    13741 caataatacg aaaatcaatt ctggcggaga
//
LOCUS       DAZUGK010000117        13730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZUGK010000117 DAZUGK010000000
VERSION     DAZUGK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.118"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..257
                     /locus_tag="ACHY5W_003046"
                     /pseudo
     CDS             1..257
                     /locus_tag="ACHY5W_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(295..2028)
                     /gene="ggt"
                     /locus_tag="ACHY5W_003047"
     CDS             complement(295..2028)
                     /gene="ggt"
                     /locus_tag="ACHY5W_003047"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFS4447910.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            2148..2588
                     /locus_tag="ACHY5W_003048"
     CDS             2148..2588
                     /locus_tag="ACHY5W_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFS4447911.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            complement(2575..3318)
                     /gene="ugpQ"
                     /locus_tag="ACHY5W_003049"
     CDS             complement(2575..3318)
                     /gene="ugpQ"
                     /locus_tag="ACHY5W_003049"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4447912.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWGGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTTRLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3315..4385)
                     /gene="ugpC"
                     /locus_tag="ACHY5W_003050"
     CDS             complement(3315..4385)
                     /gene="ugpC"
                     /locus_tag="ACHY5W_003050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFS4447913.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(4387..5232)
                     /gene="ugpE"
                     /locus_tag="ACHY5W_003051"
     CDS             complement(4387..5232)
                     /gene="ugpE"
                     /locus_tag="ACHY5W_003051"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFS4447914.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5229..6116)
                     /gene="ugpA"
                     /locus_tag="ACHY5W_003052"
     CDS             complement(5229..6116)
                     /gene="ugpA"
                     /locus_tag="ACHY5W_003052"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFS4447915.1"
                     /translation="MSSSRPVFRARWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITYFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(6214..7530)
                     /gene="ugpB"
                     /locus_tag="ACHY5W_003053"
     CDS             complement(6214..7530)
                     /gene="ugpB"
                     /locus_tag="ACHY5W_003053"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFS4447916.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(7890..8639)
                     /locus_tag="ACHY5W_003054"
     CDS             complement(7890..8639)
                     /locus_tag="ACHY5W_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447917.1"
                     /translation="MHYAVVAMLEMLCCIHAYRTGQERYWFFIIFCFPVIGCVAYFVM
                     VMLPETGADRHGRTFLMRLQDKINPERHLRKLTEELAIAETNQNHYQLANELARLERY
                     HEAVPHYQQALSGIFAHEAAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADG
                     HLLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVV
                     DTAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            complement(8758..9471)
                     /gene="livF"
                     /locus_tag="ACHY5W_003055"
     CDS             complement(8758..9471)
                     /gene="livF"
                     /locus_tag="ACHY5W_003055"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFS4447918.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(9473..10240)
                     /gene="livG"
                     /locus_tag="ACHY5W_003056"
     CDS             complement(9473..10240)
                     /gene="livG"
                     /locus_tag="ACHY5W_003056"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFS4447919.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAAIWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(10237..11514)
                     /gene="livM"
                     /locus_tag="ACHY5W_003057"
     CDS             complement(10237..11514)
                     /gene="livM"
                     /locus_tag="ACHY5W_003057"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFS4447920.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(11511..12437)
                     /gene="livH"
                     /locus_tag="ACHY5W_003058"
     CDS             complement(11511..12437)
                     /gene="livH"
                     /locus_tag="ACHY5W_003058"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFS4447921.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIISALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(12485..13594)
                     /gene="livK"
                     /locus_tag="ACHY5W_003059"
     CDS             complement(12485..13594)
                     /gene="livK"
                     /locus_tag="ACHY5W_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFS4447922.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGEAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         3236 a         3937 c         3514 g         3043 t
ORIGIN      
        1 aaagcgaacc attaccttga tgatgcagat ctcccggtcg tcattactct acatggacgc
       61 tttagccagg gctggtattt ctgtttcgaa gcacgagaat ttctcgaaac tggagatgag
      121 gccgcgcgct tagctggtaa cgcacctttt attattgata aagacagtgg tgaaattcat
      181 tctctgggaa cggcaaagcc gctggaagaa tatctacagg attacgaaaa aaaaaggcta
      241 ccttcggctt gccctgacaa aatagccctc ttcccacgaa gagggccgct aaccttaata
      301 ccccgcagtt aaatcatcca ccgagcgcgg gtcggatgca ccatacagct caccgtccgg
      361 cccaaccata atgctttgtg tactgcccat cgcctctttc agcgccactt tctgaccttt
      421 tgcttccagc agcttgagcg tatccgggct aaaccctttt tcgacacgca gctcgtccgg
      481 caaccactga tggtggaaac gcggcgcatt ggtcgcttcg gcgacgttca tgccataatc
      541 gatgctattc accaccattt gaagcactgt agtgatgatc cggctcccgc cagggctacc
      601 ggtgaccagc caggttttac cgtctttcac cacaatggtg ggtgacatcg atgacagcgg
      661 gcgtttgttc ggcccgacgg cgttggcatc accgcccacc agcccgtaaa cgttcggtac
      721 gcccggtttg gcggagaaat catccatctg gttattaagc agaataccgc tctcgcccgc
      781 gacaatgccc gtaccgaagg tggtgttcag cgtataggtc accgccaccg cgttaccatc
      841 tttatccacc actgagtaat gggtagtttg attactctca taaggcgcaa gcttgccagg
      901 gcgaatctcg ctggacggtt tcgctttatt gatatcgatt tgctcggcaa tagatttggc
      961 ataggcttta ttggtcagcg cctgccacgg gactttcaca aaatccgggt cgccaagata
     1021 ttccgagcgg tcagcgtagg catatttctc cgcttctgcc atgatctgca tcgcgtcggc
     1081 gctgccaaaa ccgtatttct tcatatcgaa gttttccaga atattgagga tttgtacgat
     1141 atggatcccg ccggaggatg gcggtggcat ggagtaaacc tgatacccgc gataatcgcc
     1201 gcttatcggt gtgcgttcga ccgctttata cgccgctaaa tcttctttag tgatcaggcc
     1261 accgtttttc tgcatctcct gggcaatctg ttccgctatc gtgcctttgt agaattcgtc
     1321 cgggccgttt tcggcaatca tctccaggct ctttgccagg ttcgcctgca ccagcttgtc
     1381 gccctttttc agcggctcgc cctctttcca gaagatagcc ttgctgtttt cgtgattcgg
     1441 cagcacttcg ctcccgtagg ttttgagatc gtcagccagc gcgtcgttaa cgataaaacc
     1501 atcgcgtgcc agtttaaacg ctggctgcac cactttgttc aacggcattg tgccgtattt
     1561 atccaacgcc agcgagaaac cagcgaccgt accgggcgtg ccggaagcca gatgcgaagt
     1621 gagtgatttt ttgctgtccg ggttgccctg gtcatcaagg aacatatcgc gggtcgcttt
     1681 ggcgggtgcc atttcgcgga aatcgatagc ggtggtattg ccatttttcg agcggatcag
     1741 cataaaacca ccaccgccca gattccctgc ctgcggatgc gttaccgcca gcgcgtagcc
     1801 cacagccacg gcggcatcaa cggcattccc gccctccttg aggatatcca cccccacctg
     1861 agtggcagta gcatctacag atgccaccat cccctgtttc gcgcgtaccg gatggaagac
     1921 atcttcctcc acgccgtaag aaacaggagg cgcggcggcg gcgctaaaac aacttcctga
     1981 gagcagagca gcaatagcca cccggcgtaa aaacgtcggt tttatcatcg ttattctcca
     2041 gagattaagg ggcaacccca gctaagcctg gtatataact ctgaattaat cctgatgatt
     2101 gcaggaaagg agtaaactta aagaatatct tcagaggagg atacgcgatg aaacgacttc
     2161 tgattcttac ggcactcctg ccgtttgtcg gctttgcaca gcccattaat actctgaaca
     2221 accctaacca gccggggtat cagatcccca gtcagcagcg gatgcaaacc cagatgcaga
     2281 ctcagcaaat ccagcaaaaa gggatgctga atcagcaact gaaaacgcaa actcagttgc
     2341 aacaacagca tttagaaaac cagataaaca ataattctca gcgggtgttg cagtcacagc
     2401 cgggggagcg aaatcccgcc cggcagcaaa tgctgcccaa caccaatggc gggatgttaa
     2461 acagcaaccg taatccggat agttcgttga atcagcagca tatgttgccg gagaggagaa
     2521 acggcgacat gctgaatcag cccggcacgc cgcagcctga tataccgttg aaaactattg
     2581 ggccgtaaag ttcggaccaa tcacatcaat cgcatcggta cagatgcaat ccacgcccca
     2641 gcgcagcaac tctgccgcgc gctggggttt attgacggta taaaccagaa tccgcagtcc
     2701 agcatctttc aactgcatta ctcgcgcttt atcaagcaac ttatgattga gatgaataga
     2761 gacgcagccc agccgcgtag tcagttcgcg ccagtcgtcg cgccattcat ccagcaacaa
     2821 accgcgcggc agttctggta ctgcctgttg tgcggcttct aaagcatcaa tttcgaacga
     2881 tgacagcagc ggcggcgtca taccgcccca cagctcgcgt gccgccagcg ccaccatttt
     2941 gcccgttaat ggcccggtgc cagtggtggg tttgatttcg atattcgcca tcatcccatg
     3001 ttcgcgacag cgttccgcca cctgcgaaag caacggcagc ggctcacctt taaacgcttt
     3061 gccgtaccaa ctgcctgcat ccacacgtag taaatcctgc cagttcagtt cacccgcgac
     3121 gccccagccg ttgctggtac gttcgagatt gtcgtcatgg agcaggaaga tttcgccatc
     3181 tttcgacaac ttcgcgtcaa attcgatcat cttatgaccg tattttgccc cgacgtcgat
     3241 tgccgccagg gtgttttccg gggccagctt accgccgcca cgatgagcga cgatgcgggg
     3301 ataaggccag ttactcatac tcgttgtcct gtttcaccat caaaaagatg cagctgattt
     3361 tccgccagat gcagccacag cgtgctgcct gccgtcgggc gctcctgatg cgccagtcgc
     3421 accaccagct tctgttcgcc ccagcgtccg tgcgccaggt tatctgcgcc gaggatctcc
     3481 agcgtgtcca tcaccagcgg aacgccacct tctgcctgcg agcttagcgc aatatgttcc
     3541 gggcgaatgc cgagagtcat tttacgcccg gcatactgac ggtagccacc gtttagcggc
     3601 agcacaatac cgccgtccag ttcgaaatgc gtgccttcgg tattcacgcg gcctgtcagc
     3661 aggttcatcg ccgggctgcc gataaaactc gccacaaaca ggctggcggg cttttcgtag
     3721 acttcaactg gcgtgccaat ctgttcggca acgccgccgt tcatcaccat tactcgctgg
     3781 gcgagggtca tcgcctcaac ctgatcgtga gtaacgtaga gtgaagtcgt tttcaggcga
     3841 cggtgcagct gttgcagttc aagacgcatc tgtacgcgca gcttggcatc gaggttagag
     3901 agtggctcat caaacaggaa caccgccgga tcgcgcacaa tcgcgcggcc catcgccaca
     3961 cgctgacgct gaccgccgga aagctcgcgc gggcggcgct taagcagacc gtccagttcc
     4021 agaatgcgcg ccgcttcttt aacgcgctcg gcaatttgct gcttgcccat gccgcgaatt
     4081 ttcagccccc acgccatgtt ttcttcaaca ctcatatgcg gataaagcgc gtagttctgg
     4141 aacaccatcg caatcccgcg atctttcggc tccatttcgg tcacccgctg gtcgttgatc
     4201 cagatatcgc cttccgtcac ccgctccagc ccggcaacca tgcgcagcag cgtcgatttc
     4261 ccgcagcctg acggcccgac catcacgata aattcgccat ccgccacatc aagggtcagc
     4321 ggtttaatca cctgggtttt gccatcccag cttttggtta ctgcctgtaa tttcagtcct
     4381 gccatcttat ttctcactat cgaccaggcc gcgcacgaag gcacgctgca tcactaaaac
     4441 aatcactact ggtgggatca gggttaacaa catcgccgcc atcactgagt tccattctgt
     4501 ggtgccttcg ccagtggcga tcatcccttt gatccctgcc acggtggtgc cgagatccac
     4561 gtcggtaata atcaacaacg gccacaaata ctggttccag ccgtaaataa aggtgatcac
     4621 aaacaacgcc gccagattag ttttggacaa cggaaaaacg atgtcgcaaa agaagcgcat
     4681 tggcgatgcg ccgtcgatcc gcgcggcttc caccagttca tccggtaggg tcataaagaa
     4741 ctggcggaaa agaaacgtcg cggtcgccga ggccatcagc ggcagcgtta aacctgcgta
     4801 gctgtcgagc atcttcaggt tggcgatgac ttccaccgtc gggaagatac gtacttcaac
     4861 cggcaacatc agggtgataa aaatcatcca gaaaaagagg ttacgcagcg gaaaacgaaa
     4921 ccagacaatg gcgaatgccg agagcatcga gacggtaatt ttgccgagcg taatgctgaa
     4981 cgccatcacg aagctgttaa gcaacatccg ccagaacggc gcactatttg tgcctacccc
     5041 gtttacccag atgttgtgga tgttttccag cagatgcgtg ccggggatta gcgtcatcgg
     5101 cgcggcgtag acggcttgtt tatccagcgt cgccgcgaca aacgccacgt acagcgggaa
     5161 gaggatcacc gcgatcccga ggatcagcat ggtatggctg aatatcgtca gccacggacg
     5221 gttctcaatc attggtaacg caccttgctt tcaacatagc ggaactgcac caccgtcagc
     5281 acgatgacga ggaacatcaa caccaccgac tgtgcggcag acgaagccag atccagtccg
     5341 gtaaagcctt cgcggtagat cttataaatc agcgtcgtgg tggcctgaac cggcccgccg
     5401 gacgtggcgg cgtcgatcac cgggaaggtg tcgaagaagg catacaccag gttcactacc
     5461 agcaggaaga aactcactgg ggcgataagc ggcagcgcaa tcttaaagaa gcggcgaatc
     5521 ggccctgcac cgtcgatggc tgcggcttcg atcaacgaac ggggaatgga ttgcagcgcg
     5581 gcatagaaga acaggaagtt gtagctgatt tgcttccata ctgaggcaaa caccaccaga
     5641 aacatcgcct gaccgctatt ttgcgcgtgg ttccagtcgt agccgaactc agcgagaaaa
     5701 taggtgatca gcccgcgacc ggggttaaac aggaagatcc acaatacggc ggcaacggcg
     5761 ggagccacgg catacggcag cagcatcaag gtttgataga aacgactgcc gcgcacgatg
     5821 tactccacca gcgccgcaaa gaacagcgat accagcaaac cgctgacggt gacaaaggtg
     5881 ctgaatttta tcgtcgtcca gaaggagtcg agatagtagc tgtcatgaaa cagcgcgacg
     5941 aagttatcca ggccgacaaa ctggctggag aaaccaaacg gatcgacgct ttgcagcgag
     6001 taccacaacg cttcgcccgc aggccagata aaaaagataa cggtgatgat gagctgcggc
     6061 gcgaccagca gataaggcag ccagcgcgcg cggaacaccg gacgggatga tgacattacg
     6121 gttaattcct gaactgtgcc ggatgcgctt cgcttatccg gcctacacgc gatgccaggt
     6181 cagataagcg aaacgcatcc gacattacac tgattaagac ttcgtcgatt tctcaaagcg
     6241 gcgcagtaac tggttcccac gctcaacggc ggtatccagt gcctgctgcg gtgtcttctt
     6301 accggtccac acgctctcca gctcttcatc cacaatcacg cggatctgcg gcatgttgcc
     6361 cagacgcagc cctttggtga acggcaacgg cggcttgttc agcatctgac gcgtcgcggt
     6421 atccgcccct gggtttttct cataaaagcc ctgctcacgg gtcaggtcat acgctgcttt
     6481 ggtgattggc agataaccgg ttttctgatg ccactcggca gcgttttctg gcttcgcgag
     6541 gaagtcgagg aacttcgcca cgccggtata cgtttcttta tctttgccct gcatcaccca
     6601 caggctggcc ccgccgataa tggcgttttg cggtgcatct ttcgcatcgg cgtcgtaagg
     6661 catcatgcct acgccatagt taaatttggc gtactcacga atattagcga gagaaccgga
     6721 agaggcggtg gtcatcgcgc aatcaccgtt atagaacttc tcggtggatt catccttacg
     6781 cccgacgtag ctgaaatcgc ccttcttgtt catctcttcg agcatggcga tgtgtttcac
     6841 ctgctccggc ttgttgaact ccagcaccgc atccgtgccg tcaaagccgt tgtttttgct
     6901 ggcaaacggc agaccgttcc aggcgctaaa gttttccagt tggatccagc cctgccagcc
     6961 gctggcgtag ccgcacttca ttccggaggc tttcagtttt gcagaatagt ccgccagatc
     7021 ctgccaggtt ttcggcggct gttccgggtc taatcctgct ttcttgaagg cgtctttgtt
     7081 gtaatagaga acgggggtcg agctgttgaa tggctgggag agtaagtggc ccgttttgct
     7141 gtcggagtag taaccggaaa ccgtcggcac aaactgcgac tcatcgaatt gaatccccgc
     7201 ctctttaaac acgtcatata ccggtttaat ggctttcgac gccatcatgg tggcggtgcc
     7261 aacttcataa acctgcaaaa tagccggagc attgccggta cgaaatgcgg caatccccgc
     7321 gcttaaattc tgttcgtagt tgcctttata ggtcggtaca attttgtaat ccgggttttc
     7381 ggcgttaaaa cgttgggcca gagaatccac ctctttaccc agttcccctt ccatagaatg
     7441 ccagaacgga atggtcgtca ctgcctgtgc attccccatt aacgccagtc cgagcgccag
     7501 tgctgaagct gtataatgta acggtttcat cgtttatctc tcttgttgta ccgaatgcgc
     7561 gaattcacgc gttttatgct cgcggggtaa catgacatgc tcgaattaca gaaaaataac
     7621 ttttttgtta catttgtaag atagtaaggt gtcagaaaga tgacaaggcg gtgacggtac
     7681 gggtgaggga aaatgggaga tggggcacgg atatcccgag aatgcactga aaagaggcga
     7741 ttaacggcca gctaatcgcc agaactcctt accatgaaga gcaaaaatgg cagcaaattc
     7801 tgcgtccagt tttgctgccc gatgagagct acatttttca attttaacaa aagattgccg
     7861 cgacactggt ctaacggcaa agaagatttt cattgcacca cactctgctt caaccgttca
     7921 ttggcggtct taatccattc ccggtggtgt tttctataat gaggtcttga gcgtttagcc
     7981 gtatcaacta cagcaacata ctgctcgttg gcttcacgta accgactcat tttcgccaac
     8041 atttccgcat agtaaatacg cgcctgcggg ccggggtaat aagaaatcaa tacttcaaac
     8101 tcgctttccg catcagcgta tttttcttgt gcggctaacg ttctggcaaa taacaaatgc
     8161 ccgtctgccg actgaaaatc agggttataa cgcatcacat cttccagcgt ctgctgacag
     8221 gcggcgaact cctgaatagc aaactgtgcc tgcgccaggc ttagcatcat tgccgccgct
     8281 tcatgggcga atatcccgct taacgcctgc tgataatggg gaacggcttc gtggtaacgc
     8341 tccagacgcg ccagctcgtt ggcaagttga tagtggtttt gattggtctc ggcaatcgcc
     8401 agctcttcgg ttaatttgcg caggtgacgt tccggattga ttttgtcctg caggcgcatc
     8461 agaaaggtac gcccgtgacg gtcagcgccc gtttctggca gcatcaccat gacaaaatac
     8521 gccacgcaac caatcaccgg aaaacagaag atgataaaaa accaatatcg ctcttgtccg
     8581 gtacggtaag cgtggataca gcaaagcatt tcaagcatgg cgacaacggc ataatgcatg
     8641 ggagggaacc tttccgtggc aaataagtgg tctggtttta atcctcccgg tcagttgaaa
     8701 caagtctgta tacggacgaa gagcccggca gaacgtggct gccgggtatt cgataattta
     8761 cccgcctaaa tacgcacttc tcaccgcttc gttcgccagt agtgcatcac cagtatcgga
     8821 gagcaccaca tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt
     8881 ggcgttctgc tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc
     8941 gaaaatttgc tggatgataa tcggcgcaag accgagcgat ggctcatcaa gcaacaacaa
     9001 acgcgggttg ctcatcagcg cgcgaccaat cgccagcatc tgctgttcac cgccggacat
     9061 ggtgcccgcc cgctgaacac gacgctcatg aagacgtgga aacagctcat acacccactt
     9121 tatgcgctcc tgaaactgat cacgttcggc aaaaaagccg cccatcgcca ggttctcttc
     9181 caccgtcatc cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat
     9241 gattttcgcc gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt
     9301 ggcgcgcgga tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc
     9361 aatcagcgtg acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat
     9421 tttgccgtag tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct
     9481 cacctaaata ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg
     9541 ccagcggcgt cccctgattg accacgtaaa ttcggtccga aattcccatc accagcttca
     9601 tatcgtgttc aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct
     9661 catccagctc tttcgtttct ttcgggttaa gacctgccgc aggttcgtcg agcattaaaa
     9721 tctccggctg cgtcaccatg cagcgggcga tctccagacg gcgctggtca ccgtaggcca
     9781 ggttgctcgc ctggcggttg gcgtgttcca gcaaaccaat gcgctcaagc cagatggcgg
     9841 cgcggtcgag cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga
     9901 acagcccggt tttcagttgc tgatgctgcg ccaccagcag gttttcaatt accgtcattt
     9961 cacggaacag acgcacatgc tggaaggtgc gcaccacgcc catgcgggcg atttgctgcc
    10021 ccggcaaacc ctccagatgc tgatcgcgca gtaaaatagt gccgccggtg ggtttgtaga
    10081 atccggtcag gcagttaaag accgtggttt ttccggcacc gttaggaccg attaacgaga
    10141 cgatctcctg cgggtacagt tcaagattga cgttgttcac tgccagcagg ccgccgaagc
    10201 gcatcatcag gccgttaaca gataataatg gctgactcat gcctgctctc ctttcgctgc
    10261 gccgtttttc agcttgagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat
    10321 catcatcagc accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat
    10381 caactcgcgc gacaccacca gcaaaattgc cgccagaatc accgcaaatt gcgagcccat
    10441 accgccgagc accactatcg ccagtacaaa ggctgattca gcaaaggtga aggattccgg
    10501 gctgacaaag ccctgacgcg ccgcaaacag cgttccggca aaacccgcaa acgcggcgct
    10561 gatggtaaag gcggtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat
    10621 ttcgtcttca cgcaacgctt cccaggcgcg acccagcggc atccgcagca ggcggttaat
    10681 gacaaacagg cttaatacca ccagcagcaa cgccaccagg tagaggaaga taacgcgatc
    10741 ggagggatcg tatttcaggc caaagaaatt actgaacgtg tcccagccgc cttcacgggc
    10801 ggtacggctg aactcgaggc cgaagaaagt cggtttcggg atctgactga taccattcgg
    10861 gccgccggta atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag
    10921 ggtaacgatc gccagatagt cgccgcgcag gcgcagcacc ggaaaaccga gcaggaagcc
    10981 cgccgccgct gccattaacc cggcaatcgg cagacaggtc cagaagccca ggccgtaata
    11041 gtgattgagc agcgcaaagg tgtaagcgcc gatggcgtaa aagccgccgt aacccagcac
    11101 cagcaggcca gaaagcccta ccaccacgtt cagcccgagg ccgaggataa tgtagatcat
    11161 ggtcaaggtg gcgatatcca ccgtcccgcg tgaaaccata aacggccacg ccaccgcaag
    11221 caccagcagc gccacgagaa acagcttttg cttcaccgtg gagccatcaa tcgcgggcag
    11281 aataaacttc ggtccggaaa cgcttttcaa ccctttctgg aaagccggtc gcaaaagctg
    11341 gaagaaaaag accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct
    11401 atcgaccacc agtttggtgc catccagctc cagttgcacg cccataaaga cgcccgccag
    11461 cacaaagaac atcgcggcag agagcagcgc cattgcaata tgcatcggtt tcatactttc
    11521 tctacctccg ggcgccccag aataccggtc ggcatcacca gcagcaccag aatcagcagg
    11581 gcgaatgaga ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca
    11641 atccccagaa tcagcccgcc aatcatcgct cccggaatgc tgccaatccc accgagcacc
    11701 gcagcggtaa aggctttcat cccggccata aagccgatgt aggggttaat gacgccgtag
    11761 aactgaccga gcagtacgcc cgccaccgcc gccattgccg cgccaatcac aaaggtcagc
    11821 gcaatcaccc ggtcggtgtt aatgccaagc aggctcgcca ttttcagatc ttcggcgcag
    11881 gcacggcagg cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg
    11941 aggaaagtaa caatccagat caccgcctgc atggtagtaa tagaggcaga gaagttttcg
    12001 ctatggccca ccacccactg accgttaaac aggctcggca gcgccacgtc gcgcgaacct
    12061 tcggtcaggc tgacgtagtt ttgcaggaag atggacatac cgattgccga gatgagtgca
    12121 atcaggcgct tagagttacg caccgggcgg taagccaccc gttcgatact ccagccgtag
    12181 gcgctggcaa tgacgattgc gccaacgaat cccgccgcca ccagcagcca gccggtatca
    12241 atgcccagca tcatcagcgc ggagatgatc ataaaggaga catagctacc aatcatataa
    12301 acctcgccgt gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg
    12361 gctatcagcg cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat
    12421 aaaaactgct cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg
    12481 attttcactt ggctgccgtg gatgaaccgt cggcgtgcca ctggaagaca ccaaaatcaa
    12541 atcccttaag atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt
    12601 tagcttttaa atctttcacc agcgccagcg gctcatcgct gccggtacgc tcaagggcag
    12661 ttgccagaga ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct
    12721 tgtctgcttt cagcgcatca acgatgccct ggtttgccgg gtcctggtca tagcgttttg
    12781 gcatagtgac caacatgcct tcggcggctt cgccagcaat gttcgacagc gacgcattac
    12841 ccacaccttc cggccccata aactgggttt tcaggccaac ggaacgggcc tggcgcagca
    12901 tctgccccat ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca
    12961 ggcgtgcgat cagcgcggag aaatctttct cgccagcggt aatgccgtcg aagaagacga
    13021 cattggcgtt agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact
    13081 gttgtttgtc gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg
    13141 ccgccgttgg cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc
    13201 gttgggtcag ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcat
    13261 agatatctga cgcaggctgg gtagaagaag aacacagatg accaataacg tatttaatgc
    13321 cgtcattaac gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat
    13381 attccacgcc aaccagctta tcgcctttaa ttcccccttt ggcattaatg tctttaatcg
    13441 cctgacgcgc gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc
    13501 cgacaacggc aactttaata tcgtcggcta aagcggtgtg tgaaattgcc agtgcaatca
    13561 tccctgcgat gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt
    13621 gtgttgcttt gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt
    13681 tttgcctgtc tgattaaggg tttagcgcag tattttgtga taatagcgat
//
LOCUS       DAZUGK010000118        13510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZUGK010000118 DAZUGK010000000
VERSION     DAZUGK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.119"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(247..762)
                     /gene="pgpA"
                     /locus_tag="ACHY5W_003060"
     CDS             complement(247..762)
                     /gene="pgpA"
                     /locus_tag="ACHY5W_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4447923.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(740..1717)
                     /gene="thiL"
                     /locus_tag="ACHY5W_003061"
     CDS             complement(740..1717)
                     /gene="thiL"
                     /locus_tag="ACHY5W_003061"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFS4447924.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1795..2214)
                     /gene="nusB"
                     /locus_tag="ACHY5W_003062"
     CDS             complement(1795..2214)
                     /gene="nusB"
                     /locus_tag="ACHY5W_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4447925.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2234..2704)
                     /gene="ribE"
                     /locus_tag="ACHY5W_003063"
     CDS             complement(2234..2704)
                     /gene="ribE"
                     /locus_tag="ACHY5W_003063"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFS4447926.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2793..3896)
                     /gene="ribD"
                     /locus_tag="ACHY5W_003064"
     CDS             complement(2793..3896)
                     /gene="ribD"
                     /locus_tag="ACHY5W_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFS4447927.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3900..4349)
                     /gene="nrdR"
                     /locus_tag="ACHY5W_003065"
     CDS             complement(3900..4349)
                     /gene="nrdR"
                     /locus_tag="ACHY5W_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4447928.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4500..5039
                     /locus_tag="ACHY5W_003066"
     CDS             4500..5039
                     /locus_tag="ACHY5W_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS4447929.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISMDQLGFVRIHDIQPVMQ"
     gene            5338..6222
                     /gene="tsx"
                     /locus_tag="ACHY5W_003067"
     CDS             5338..6222
                     /gene="tsx"
                     /locus_tag="ACHY5W_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFS4447930.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6399..6746)
                     /gene="yajD"
                     /locus_tag="ACHY5W_003068"
     CDS             complement(6399..6746)
                     /gene="yajD"
                     /locus_tag="ACHY5W_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFS4447931.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6875..7846)
                     /gene="secF"
                     /locus_tag="ACHY5W_003069"
     CDS             complement(6875..7846)
                     /gene="secF"
                     /locus_tag="ACHY5W_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFS4447932.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7857..9704)
                     /gene="secD"
                     /locus_tag="ACHY5W_003070"
     CDS             complement(7857..9704)
                     /gene="secD"
                     /locus_tag="ACHY5W_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFS4447933.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9732..10064)
                     /gene="yajC"
                     /locus_tag="ACHY5W_003071"
     CDS             complement(9732..10064)
                     /gene="yajC"
                     /locus_tag="ACHY5W_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4447934.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10087..11214)
                     /gene="tgt"
                     /locus_tag="ACHY5W_003072"
     CDS             complement(10087..11214)
                     /gene="tgt"
                     /locus_tag="ACHY5W_003072"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4447935.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11270..12340)
                     /gene="queA"
                     /locus_tag="ACHY5W_003073"
     CDS             complement(11270..12340)
                     /gene="queA"
                     /locus_tag="ACHY5W_003073"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4447936.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12433..13014
                     /gene="acpH"
                     /locus_tag="ACHY5W_003074"
     CDS             12433..13014
                     /gene="acpH"
                     /locus_tag="ACHY5W_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFS4447937.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13035..>13510)
                     /locus_tag="ACHY5W_003075"
     CDS             complement(13035..>13510)
                     /locus_tag="ACHY5W_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447938.1"
                     /translation="FSQEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQCLHNV
                     ARDGSPRYAGLLQQIASKCAILQDFDQQLFWRTFTGGVLHRHISVKSEPEKTDVWRFS
                     TQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3339 a         3625 c         3279 g         3267 t
ORIGIN      
        1 aataattttg atgatatttt tcgtcaacag tcagagaata tgagcaatta ttatgagttc
       61 gtcgactgac gatatttcag tgtcattgaa ttatgagtga caaagataca ccttgaaata
      121 tcgccgcata gtatattcgg tgatgtcggg ttttacttta acagagtgcc ccaaaattgg
      181 ggcactgttc atttataaat caaaccgggt ggtctggaat cgcctacacc tcccggtcaa
      241 tggcatttaa agaataccca gcggccagtg atgaccgata aaatacagga tgcctgcgga
      301 aatcaccccg gcgacaatat catcgatcat gatccccatg ccaccatgta cattgcgatc
      361 aaaccagcgg atcggccacg gtttccacat atccaggata cggaaaatca caaatccggc
      421 ggcgacccac tgccagtcat tggtcggcag cgccatgagc gtgatccaca taccaataaa
      481 ttcgtcccag acaatgctgc cgtgatcgtg cacgcccatg tctttcgccg tttgatgaca
      541 aagatagacg ccgatacaga tccccagcat caccaccagc gaatagagct gccagggcaa
      601 aaaggtcatc agataccaga acggaatcgc tgccagcgag cccatcgtcc caggaacgat
      661 cgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac tcatcttgag
      721 gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc cagtctaacg
      781 tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc atttgcccga
      841 tacaggtaaa cggtacgccc agatgaccga gagccacatc cagcgcgcca cggttcagtt
      901 ccggcacagt gaaacacaac tcgtaatctt caccgccaga gagcgcccag cgcagcgcct
      961 gttccggtac aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac
     1021 gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga
     1081 gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg
     1141 gacggagatg acgtttgatc aagtagtccg catctttagc atcggcaacc tgcaaacggt
     1201 tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca tagatccagt
     1261 cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac
     1321 ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat
     1381 aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg tctacgtccg
     1441 gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc agatcgctta
     1501 ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg aggaaatggt
     1561 tgcccgccac cagcgtatca gtgctgatcg ccaaggtctg tttctcgggg atattgagaa
     1621 gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt acacggtcaa
     1681 aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca gcaggtaaaa
     1741 aagaaaaggc cggcgatcgc ggaacagtct tccgtgaatc taccggcctg gatattactt
     1801 tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga acttatggct
     1861 gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg
     1921 cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt tttctacctg
     1981 tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga ggtatgcggt
     2041 attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt cttttacatc
     2101 ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca actgccagga
     2161 gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt tcacggattt
     2221 ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca agcgcggtca
     2281 gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc gcttgttcaa
     2341 tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg
     2401 ccaggccgtt gcttgcacca ccagcgacat attcaaaatg ggcagtgcca ccacgaataa
     2461 ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca cccgccgcca
     2521 gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct tttacctgac
     2581 cgatacgttt cagtgcatca attgcacctt ccagcaggct gtcattgata aagttgttga
     2641 aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct tcaataatgt
     2701 tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt agcataatat
     2761 ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg caggcaaaca
     2821 tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcggc taatttctca
     2881 agccctggca gtgtgcataa tccacgggcg tcgctgccta atagtttagg tgcgatatag
     2941 acaatcagct catcgactaa acccgcctgc agcaatgcgc cagcgagcgt tggccccgct
     3001 tccacccaga tgctgttaat ctgctgttta cccagttgca tcattagtac aaccagatcc
     3061 agatgacctt tatgctctgg aatcaacaag gtacgtaccg tttccggcca ctcacgagaa
     3121 tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg atgttccggc
     3181 gtcacgcgat tttggctatc aatcacaata cgtatcggct gacggagatt ttgttgcgga
     3241 tagagcgcct gagtttgttc atccagttca gaccaacgca ccgttaaggc cggatcatcc
     3301 gccagcaccg tggcgctgct ggttaaaatg gcatgacttt gtgcgcgcag tcgttgtacg
     3361 tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat cgccgtgcgg
     3421 ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg catccgcttg
     3481 agaaagcctt tattcaattg ctcggcttca ctcatcatca ggccgtggct gacgtcaatg
     3541 ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttcgg atcttgcatc
     3601 gcagcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg tggcgtacga
     3661 ccatgatggc tacagggttc gagtgtgaca taggcggtcg cacctttggc tttttcaccc
     3721 gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca
     3781 ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg gtgggtggta
     3841 aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct
     3901 tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa actgcggtag
     3961 acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc catcaccaga
     4021 ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata
     4081 tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag cgcccgcagc
     4141 attccgctac gtaatttttc ttcattaaac ggttcacgca cgtcgttgct ttttacaaca
     4201 cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca caccagacac
     4261 tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat taccttagtg
     4321 tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt taacagaaat
     4381 ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat aaaggatcga
     4441 tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag ggacgaccta
     4501 tgaatattaa cgtttttcga ctgatcctgc tgggaagcct gttcagcctt agcgcctgtg
     4561 tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac caggagatga
     4621 cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca
     4681 gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa agccagatcg
     4741 gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc accctgacac
     4801 tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact gttgaatatg
     4861 ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtacagaat caattgatta
     4921 atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag ttaaagggga
     4981 tttctatgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt atgcagtaaa
     5041 cgtatagcgg ggcgattttg tgcgtcccgc aacatctttc cccgtctttt tgttactccg
     5101 cttacatcac ccagattgat agtaaaagtt tgcaacaagg gtgaaagtca gtacaatccc
     5161 tgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg
     5221 aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg aaatatttag
     5281 aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg catacatatg
     5341 aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt tactgtcaac
     5401 gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag cgttaacgtt
     5461 gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta ccttgagtac
     5521 gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc gccggtattc
     5581 ttcggcggta actccgatgc aaaaggtatc tggaaccacg gttctccgct gtttatggaa
     5641 atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt cggtccgttc
     5701 aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa agatggtcgc
     5761 cagagcacct ggtacatggg tctgggtacc gatatcgaca ctggcctgcc gatgagcctg
     5821 tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag
     5881 tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct gtggggcggt
     5941 cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg ggatgacagc
     6001 ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc cagccatatt
     6061 ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg gcacgacggt
     6121 ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa cgttcgctct
     6181 accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa atgccgggat
     6241 ttattcccgg catttctgat tgttaacaac gtgcgctttg ttcatgccgg atgcggcgta
     6301 aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa taacgtaggc
     6361 ctgacaagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt gttcatcatc
     6421 gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc
     6481 cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg ctcatgatcg
     6541 tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt ggtatggtcg
     6601 tgatcaatgt ggtgaacagt aagttcacgc aggttggaat aaacaaactc gcgggaacag
     6661 cgaccgcaga cccacggata tatttttaat gctttttcgc gatagccgct ttctaaccgc
     6721 gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta gggttacgcg
     6781 cagtttccca agatgcgggg attttcgcaa tctccataca tcaaaaaaaa tcccgatctt
     6841 ctgaccggga ttttcaacat caacgtgaaa ttgattaagg cagaattgac ggctgatccg
     6901 ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg cttcataccc agtttcagag
     6961 ccaacgcaga tgctacgtag atggaagatg cagtgccgat ggaaacaccg ataagcatgg
     7021 tcagcgagaa gccttccagt accggaccac cgaagaggta cagcatcagg ataaccatca
     7081 aggtagtacc ggatgtgatc aaggtacgat gcagcgtctg ggtcaaggac acgttaaaga
     7141 tttcgtaagg cgtaccgcga cgaatcttgc ggaagttttc acgaatacgg tccgatacca
     7201 cgatactgtc gttaagcgag taaccgataa ccgacatcaa cgatgccaca atggtcaggt
     7261 caatctcgat atggaataac gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg
     7321 caataaccac ccctgccgcc agtcgccact caaagcggaa acctacgtac acgaggatag
     7381 acagcagcgc tgccatcaac gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg
     7441 gaccgacgaa ctcaatacgc ttcactgctg cattctgatt ggtggattca ttaatcacct
     7501 tcagaacctg gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga
     7561 ccatgatgtc gtggctgcta ccaaagtttt gcagcatcgg ctcttcaaaa ccggcttttt
     7621 gcaatgcatc acgcattacg tcaatttcag ccggtttttc gagcgtaatt tcgataaccg
     7681 taccaccggt gaaatccagc ccccagttaa agccgcgcac gcccataata acgatagcag
     7741 cgattaacag cagaccagag atgccgaaag cccagtagtc ccagcgcata aagtcataga
     7801 ctttacggcc gtggtttagt tgttcaacag tatattcctg tgccacatcg cactcctcag
     7861 attgacagct tcttgacgcg cttgccgcca tatagcaggt ttacgatggc acgcgtaccg
     7921 acaatcgcgg taaacatcga cgtcgccaca ccgataccgg tagtaatcgc gaacccttta
     7981 attgccccgg tacccactgc gtacaggatg atgactttaa tcagcgtggt gatgttcgca
     8041 tcgaagatag aactgaatgc gccacgataa ccttcatcaa ttgcctgttg aacagtacgt
     8101 ccgttgctca actcttcttt aatacgttcg ttgatcagta cgttcgcatc gaccgctacc
     8161 gcaagggtta agacgatacc cgcgatacct ggcatactca gtgttgcgcc tggcaacagc
     8221 gacataatac cgacgatcaa gatcaagttg gcaatcagag cactggtcgc aatcagacca
     8281 aacttcttat agaagatgat catgaacaga atagaaacca gcagaccggc aaggcaagcc
     8341 tccagcccct gctcaatgtt ctgcataccc agggtcgggc caattgtacg ttcttcaaca
     8401 atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg acgggcttcg
     8461 ttcgggttgt tgatgccggt gatacggaag ctgttaccca gacgagactg gatgttggcg
     8521 atgttaatca cctcttcctg tttcaccaga accgcacgac catttgcatc tttcttaccg
     8581 ctgtctttgt actccacaaa cagggttgcc atcggtttgc cgatgttgtc cttggtgaag
     8641 ttagacataa tgttgccacc agcgctatcg agcgagatgt taacctgcgg ctggttgtat
     8701 tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg tcagaattac gcgtttgtac
     8761 agcacaaccg gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg
     8821 gatgccgcag cggcctgatc aacgttggtg tttaccagac ggaattccag cgttgccgtc
     8881 gcacccagaa tctctttcgc acgcgcagtg tcctgaatac ctggcagttc aacaacgata
     8941 cggtcagcac cctgacgctg aaccaccggc tccgccacgc caagttggtt tacacggtta
     9001 cgcaggatat tgatgttctg ctgcaccgca tattcacgcg cttcactcag acgagcatcg
     9061 ctcattaccg cacgcagctg gttgctgccc tggctactaa tcaccaggtc cggatggcgc
     9121 ttgctcagat acgcaatggc ttcatcacga gctttagcat cgcggaaagt gatgctcagg
     9181 ccgtagttgt tttctttacg aacggtggta tacgggatgc ctttttcgcg caggtcgctg
     9241 cgcaggctat cgatattttg ttcctggagt ttgccaagcg cggtgtccat atccacttcc
     9301 atcaggaagt gaacgccgcc acgcaggtca aggccgagct tcatcggctc agcgtgaata
     9361 gctgccagcc agcgcggcgt tgccggggca aggttaagcg ccacgacgta tttgtcaccc
     9421 ataacgccca ttaatgcttc acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca
     9481 agaatagcgc cctcttccag tgccacagac ttagcagtta ttttttcttc ttgtaacgtt
     9541 ttctggacct ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc agtgatctga
     9601 acagccggat cctcaccaaa caggttggga agcgcataca gcagaccgat gacaatcacc
     9661 acgatcagca tgacgtactt ccacaaagga taacggttta acacggcaat tcccttaggg
     9721 aaaaatttta attacagcgc cttcatggtg cctttcggca ggacggcagc tacgaagtca
     9781 cgtttaataa ctacttcagt ggtgtcattc agcgcgatag caatgtagcc gttttccgct
     9841 actttggtta cgcgaccgac caggccaccg ttcgtcagaa cttcatcacc tttggcaatg
     9901 gagtccatca gctttttgtg ttctttggtg cgcttctgct gtggacgcag gatcatgaaa
     9961 tagaaaatca gaccgaatac caccagcatc aaaatcaaag acatcgggct accttgcgcc
    10021 ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt tccctcatta
    10081 ttaatattaa tcaacgttca aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt
    10141 tacgaagctc tctaatttac cctcttcaat agccttgcgt aaacccgcca tcaaacgctg
    10201 gtagtaacga aggttatgaa tggtattgag tcgcgcgcct aatatttcgt tgcaacggtc
    10261 aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac actcaggatc
    10321 gagtgggcca gtatcgctct tatacttcgc attgcggatt ttcaccacgc catcggtcac
    10381 gaacaaatga ccatttcggg cgttgcgggt tggcattacg cagtcaaaca tatcgatacc
    10441 acgacgtacg ccttcaacca ggtcttctgg tttaccaacg cccatcaggt aacgcggttt
    10501 gtctgccgga atttgcgggc atacatgctc cagaatgcgg tgcatatctg ctttcggctc
    10561 acccacagcc agaccgccga cagcgtagcc atcaaaaccg atatctacca gacctttaac
    10621 agaaatatca cgtaaatctt cgtaaacgct gccctgaatg ataccaaaca gcgcattttt
    10681 gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc caacgcagag acatctccat
    10741 ggagcgtttt gcgtaatccc aatcagcagg atacggcgta cactcatcaa agatcatgac
    10801 gatatccgaa ccaagatcgt actgaatctc catcgacttt tccgggtcga ggaaaatcgg
    10861 gtcgccgttg atcgggttac ggaagtgaac gccctgttcg gtgattttac gaatatcacc
    10921 aaggctgaag acctggaagc cgccggagtc ggtgaggatc ggtcccttcc actgcataaa
    10981 atcgtgcaga tcgccgtgca gtttcatgat ttcctgaccc gggcgcagcc acaggtggaa
    11041 ggtgttgccg aggataattt gcgcgccagt ggcttcaact tcttccggcg tcatcccttt
    11101 tacggtgccg taggtgccaa caggcataaa acaaggcgtt tccactacgc cacgatcaaa
    11161 gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc
    11221 tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc gccgcggaat tactccccga
    11281 cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa
    11341 agcgatattt ctcttctacc gctgctttat aggcgttcat ggtgtgttga taaccggcaa
    11401 aggccgaaac cagcataatc agcgtcgact ctggcaagtg gaagtttgtc accagcgcat
    11461 cgaccacttt gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt
    11521 cgatgagatc gtttttcgcc gcctgagccg cgctttccag cgaacgtact gaggtagtgc
    11581 caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct
    11641 gcggtacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg
    11701 gctggaaggt gcccgcacca acgtgcaggg tgacgaacgc catctccacg cctttggcgc
    11761 gtaatttttc cagcaaaggt tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc
    11821 ccggtttttc gctataaacg gtttgataaa gttcgcggtc agcgtcttcg tccggacggt
    11881 cgatatacgg cggcagcggc atatggccga tgctgttgag aatatccagt accgagcgtt
    11941 catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac
    12001 tttcgtcatc gcccagcagc agttctgcgc caggttttgg cgctttcgag gcgcgaatat
    12061 gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag cacttcaatc ttgccgccac
    12121 tggctttacg cccaaacaga cgcgctggga tcacccgggt attattaaaa accagaagat
    12181 cgccggggtt gagcttatcg agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg
    12241 gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catcggatag tgggcaatca
    12301 gggattcggg caattcaaag gagaaatcgg taacgcgcat gacactgact cagactaaaa
    12361 taagaggcgg gtagtctagt gccggggcgc tctccctgca acctttaccc ccctttggat
    12421 agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg
    12481 ctttccggca atttgctggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc
    12541 gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg tattgactga caatctgccg
    12601 gaagtccgcg aagcacggga atggtttcgt agtgaaacgc gccgcgttgc gcctattacc
    12661 ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc gccggacttt
    12721 ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt gccggactca
    12781 ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct ggtgcgctat
    12841 cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgtcg cccacgtctg
    12901 gatgccctgc gtgactcctg gtacgattta aacgcacatt atgccgccct cgaaacccgc
    12961 ttctggcagt tttatccgcg gatgatggcg caggcgtcac gcaaggcgct ctgaaacttc
    13021 aaactccggc gtcattactt tttagcattc gcatccagcg gggcggcata acaacggtaa
    13081 gaaaaacatt cgctggaatg attggcgata tctttaaagt tgctttcagc aaccccctcg
    13141 aacatgagta ccgttctgga caactctccg ttttcattta tatgtggaaa atcaaattgt
    13201 gtactgaatc gccatacatc tgtcttttct ggctcgctct tcacagagat atgtcgatgg
    13261 aggacccctc ctgtaaacgt gcgccagaac aattgttgat caaaatcttg taatatcgca
    13321 cacttggatg ctatttgttg tagtaaacct gcataccgtg gactgccatc acgagcaaca
    13381 ttatgcaaac actgactaaa agcatcgaac aaacaataga tgtgatcact agtgaaaaaa
    13441 ggtcttaatt ttttttgttc ttcgtcgctg taatccagga gcatctcatt gatttcttcc
    13501 tgtgagaatt
//
LOCUS       DAZUGK010000119        13341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZUGK010000119 DAZUGK010000000
VERSION     DAZUGK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.120"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..860
                     /locus_tag="ACHY5W_003076"
     CDS             30..860
                     /locus_tag="ACHY5W_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS4447939.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDNELTRRYEYARMDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMEN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1001..1774)
                     /gene="uxuR"
                     /locus_tag="ACHY5W_003077"
     CDS             complement(1001..1774)
                     /gene="uxuR"
                     /locus_tag="ACHY5W_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFS4447940.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVIREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1989..3449)
                     /locus_tag="ACHY5W_003078"
     CDS             complement(1989..3449)
                     /locus_tag="ACHY5W_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFS4447941.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTTAYQQLCERGARECVAAL"
     gene            complement(3530..4714)
                     /gene="uxuA"
                     /locus_tag="ACHY5W_003079"
     CDS             complement(3530..4714)
                     /gene="uxuA"
                     /locus_tag="ACHY5W_003079"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4447942.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLEQYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5054..6397
                     /gene="gntP"
                     /locus_tag="ACHY5W_003080"
     CDS             5054..6397
                     /gene="gntP"
                     /locus_tag="ACHY5W_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFS4447943.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6572..7474)
                     /gene="fimH"
                     /locus_tag="ACHY5W_003081"
     CDS             complement(6572..7474)
                     /gene="fimH"
                     /locus_tag="ACHY5W_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS4447944.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7494..7997)
                     /gene="fimG"
                     /locus_tag="ACHY5W_003082"
     CDS             complement(7494..7997)
                     /gene="fimG"
                     /locus_tag="ACHY5W_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFS4447945.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8010..8540)
                     /gene="fimF"
                     /locus_tag="ACHY5W_003083"
     CDS             complement(8010..8540)
                     /gene="fimF"
                     /locus_tag="ACHY5W_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFS4447946.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVATES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQILLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8550..11186)
                     /gene="fimD"
                     /locus_tag="ACHY5W_003084"
     CDS             complement(8550..11186)
                     /gene="fimD"
                     /locus_tag="ACHY5W_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFS4447947.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNQAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSHSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11253..11978)
                     /gene="fimC"
                     /locus_tag="ACHY5W_003085"
     CDS             complement(11253..11978)
                     /gene="fimC"
                     /locus_tag="ACHY5W_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS4447948.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12015..12554)
                     /locus_tag="ACHY5W_003086"
     CDS             complement(12015..12554)
                     /locus_tag="ACHY5W_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4447949.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12619..13167)
                     /gene="fimA"
                     /locus_tag="ACHY5W_003087"
     CDS             complement(12619..13167)
                     /gene="fimA"
                     /locus_tag="ACHY5W_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4447950.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     YAIGAATPGAANADATFKVQYQ"
BASE COUNT         3232 a         3453 c         3216 g         3440 t
ORIGIN      
        1 cagtgaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat acccttcaga
       61 gtcaaattat tacagataat cacttccttc atcacccaaa agtcgacaat gagttaacac
      121 gtcgatatga atatgcacgc atggatacgg aaaatatcta tcttctgcct cttgctcgtg
      181 gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca
      241 aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcgat ggtattacaa
      301 caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac gaaatatatg
      361 gtggcatatg taaaaaggcg catgctgcta cagaattagc agaaggttta cggctaaatt
      421 tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa ataacattag
      481 gccctggttg caatgttcca ggttacgccg gaaccacgat aggatatatt tcaacccttc
      541 cagtttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc tatattgatc
      601 agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc gcattactta
      661 atgctaatat tgagatggaa aatgacccaa tcattggtat agaagcctat cctggtaccg
      721 cggagatcca tgcaaaaatg ggatataaag ttatccccgg tgatgaagat gcgccactca
      781 aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat
      841 ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag gttatacaga
      901 tatttaaaca ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga
      961 aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca
     1021 gcggccatga atcaaacaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca
     1081 gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat
     1141 cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca
     1201 ggctgtcatc cagatggctg tgcaactgga tccacattgg gttgttttcc cgccactgcc
     1261 aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcggca atagcgagat
     1321 ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt tcacgctctt
     1381 ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa
     1441 actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa ggacccgcat
     1501 cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagaacat
     1561 agataccggc accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt
     1621 cacgtatcac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc
     1681 gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct
     1741 ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta
     1801 atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac
     1861 ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca
     1921 aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa
     1981 tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat
     2041 gccgtcgtca ccgcgccaac aaagtcggca ttttgcggca gatcatcggc aaaaataccg
     2101 ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg
     2161 aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg
     2221 gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc
     2281 aggtgcagac gcaccgggtc cagcagacgc tgcggtaact tctggctgcc gtccattgca
     2341 atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca
     2401 taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc
     2461 agggcgaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat ggtttcatag
     2521 ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat acgcagcttc
     2581 atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccggg
     2641 cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg
     2701 cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc
     2761 ggaacgatgc ggtcaaccat ggtgcatggg aaggtgacgt tctcttcaat ccatgccgcc
     2821 agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt
     2881 tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc acgacgcaga
     2941 cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg
     3001 tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct
     3061 ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata
     3121 ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc
     3181 tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt
     3241 tcgatcagca cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg
     3301 tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg
     3361 caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc
     3421 ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagggtg
     3481 acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa
     3541 agaaagcgcg ctggatcgcc aattcgacac cgcgaacttc ggccaggcct ttcagacgac
     3601 caattgcgga gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt
     3661 ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt
     3721 caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt
     3781 ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg
     3841 gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg
     3901 tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt
     3961 caatggtgga aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa
     4021 ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg aagacggcaa
     4081 agttttcgcg cagcttcgct ttatcgatat ctttgtactg ctccaggtgc ttacggaact
     4141 ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta cgcgtcagac
     4201 gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca atttcttctt
     4261 cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat gctgcgaatt
     4321 cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac
     4381 gagtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca
     4441 ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt
     4501 tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa
     4561 caatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt
     4621 gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat
     4681 cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt
     4741 tccacagcaa agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac
     4801 cgcagacgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat
     4861 gtcaacccga attctgaaat tggttaacca catcacaaga atttcactct cctggttgtt
     4921 gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg
     4981 caacaatggt tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt
     5041 aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggtctgat
     5101 gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct
     5161 gtccgtcggg atgctggcgg gcatggattt gatgtcgctg ctgcacacca tgaaagcggg
     5221 cttcggcaac acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa
     5281 attgatggtc gactccggcg cggctcacca gatagcgcat acgctgctgg cgcgtctcgg
     5341 tctgcgctat gtacagctgt cggtgattat catcggcctg atttttggtc tggcgatgtt
     5401 ctatgaagtg gcctttatca tgttagcgcc gctggttatt gtcattgccg ccgaagctaa
     5461 aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact
     5521 gttcccaccg cagccgggtc ctgtggcgct ggtgaatgct tatggcgcag atatggggat
     5581 ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct
     5641 gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc
     5701 ggtagatatg aacaatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc
     5761 gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata cccctgcctg
     5821 ggaagtggtt aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc
     5881 attcgtactc tttggcaccg cacgtggtca tgacatgcag tgggtgatga acgcttttga
     5941 aagtgcggtg aaaagcattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa
     6001 gcaaactatc atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa
     6061 tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg cgacgggtca
     6121 gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc ttgatccagc
     6181 aaccggtcag ctggttggcg tgaatccggc gctgctggtg ctggcgacgg ctgcgggttc
     6241 caacaccctc acccacatta acgatgcatc tttctggttg ttcaaaggtt actttgacct
     6301 gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg
     6361 gctaattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag cctgccaatg
     6421 gcaggctctg ttcggattgt cggcaaagtg cgctttgttt atgccagatg cgacgctgac
     6481 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatatttgc
     6541 gtacctgcat tagcaatgcc ctgtgatttc tttattgata aacaaaagtc acgccaataa
     6601 tcgattgcac attccctgca gtcacctgcc cgccggtacg tgcgtaattt gccgttaatc
     6661 ccagacttac cgccgaagtc cctactgctc ctaacgatac cgtgttattc gctggaataa
     6721 tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc tggtgaaaac gacgcggtat
     6781 tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga gaggtaatac cccaggtttt
     6841 ggcttttcgc acaataaacg gtaagaggaa ttggcactga accagggtag tccggcagag
     6901 taacggtgac atcacgagca gaaacatcgc agccgccagt aggcaccacc acatcattat
     6961 tggcgtaaat attccacaca aactggaaat catcgctgtt atagttgttg gtctgtcgca
     7021 aaataagcac ggcaattaat gagccagctt taatcgccac cccacccgca ctgctcacag
     7081 gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa acaacccgcg
     7141 gcgtttcgct ggtagtcgga aatggatagc tactgccact atattttacg gtcccggaaa
     7201 aattagataa cacgccgcca taagccgagc ctcgttgcag tgtgacatag tctgtaatgg
     7261 tttccggata atcgttatgg caaaagattt gcgtcgaaag atctacgacc aggttttgcc
     7321 ccacattcac ggcaggcgca aggtttacat aaacattagc gctgccaccg ccaataggga
     7381 tagcggtacc attggcggtt ttacaggcga atgaccaggc atttaccgac cagcccatca
     7441 gcagtacagc aaacagggta ataactcgtt tcattacaat tatctcttcg ggttcagctg
     7501 taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc gccatttacc
     7561 gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc cacctgaact
     7621 gttttggttg cgccagtatt caatgtgttg ccactgtcat cctgtagctc taactggatg
     7681 ttttgcgcgg tcccctggtt tttataatat ccggtactgt cggctgcccc gctgaagctg
     7741 gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac atcatgccag
     7801 gccgatgccg ccccggcaga catcagactg aaagaataaa gatcgccgag atcaaccgtg
     7861 gcattggtgg tggaaactgt gcacggtttg gcgacgacct taccgttcac cgtgatggtg
     7921 acatcggctg cctgtatcgt cgcacttgcg agcgccaata ttgccgccaa taaataccca
     7981 cgtttgcgcc atttcatgag catctccagt tactgatatt caagagtgaa ggtagccgtg
     8041 gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc gtaaaaattc
     8101 aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcggacga tggagcatta
     8161 aggagtatct gattttgctg ctcattcaga agctgtattc ccagtcccga agccgctgac
     8221 accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacgcc ggtaaaccca
     8281 acctttacgg cagaaacggc gttaccacag ggtgacagca aaatacgaaa tggaacgaca
     8341 ggagtcgttg cgccaatgtt gttaaattgc ttcgccgcgt tttccatcag atcaacagta
     8401 aaattggttg attcagtggc cacactacag ccgttatctc tgacatagcc gcggatagta
     8461 atcgtactat ccgcagccaa aacgcgactt accgccagcc acaaaaaagc gcacagaaga
     8521 taaaaaggtt tgtttctcat cacgccccct taacgacatt cagctgatag ctgggttaat
     8581 aactgctgct gactctctgg tggcagttga taattggcga cacaatgagc attttcctct
     8641 tctccccatt tcacctgaac ttttcccgct agaggcattc cgctgaggta aacctgacca
     8701 ttatccgcaa caatgccgct actatggcta ctctctgatg tcaccatcgc cccaaacggc
     8761 agcggcttat tattgtgggt tagcgtcatg agcagtttta tcccaacgcg cgctttaaac
     8821 tctgctcgca cgatcgcccc acgagtggga acaacgttag cgaccgcgtt atctaaatcg
     8881 acgttatcag ccagggtatt ggtatccagc gccactctgt tttcccgata ttcggtggca
     8941 taaggcagca cggcataacc gcgccagtcg gtacgcaccc ccgtctggtt ttcgactttt
     9001 gcatcttttg cgccaggcgc tttaacaagt accaccgtat cgtttaacgg ctgccccagc
     9061 gttacgccat tggcatgagc cagtacccca ccgctgactc cgtaatagag ctgcttaata
     9121 tcatcgctat ggctgtaacc gatattggca ttgccgtaac caccgcgata attcagcgtg
     9181 gcgtagcctg tacttccgct attaccatcg cctcccccgg catagccggt ttgcacgcta
     9241 tagctgaggt tgttgtcttc cagcaacgta ccgtatacac cagcctgatt ggtcattcga
     9301 ccgttgagat cgtgtgacat gctgtagctg gcactggcat gtcgccactg agatttactg
     9361 tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat ctgatcccgt
     9421 cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat atcttcgaac
     9481 gcagtattta atccagcctg gaattgctca tcgacattac tcgttcccca ataagtttga
     9541 tggctaccac tcaaatacag tgttgatgtg cgcccgagtt gctgagtaac ggtgagctgt
     9601 aatttcccgc gtttgttata agcgaggttg taatagtcgg tgaatttcgg cttaacctga
     9661 ataactccgt cctgtgtttc gatgttgtag ccattcattc gactgtatgt tgtatcagcg
     9721 aaattaaaat atccgctggt cgaataacgg taacccacta actgaatatt cgtgcctgac
     9781 tcattgagcg atttgttata gagaaaacgc accgattgtc cgtcatgctg actgtcatcg
     9841 ggaagtgtgg aattagcctg agtcatatcc acagacagag cgcccagtgc ccccatattt
     9901 ttcccgatac caaaattaaa agcacgataa cgatctgcca gttgcgttcc accatatatt
     9961 gtccagccag ccggaaggcc gtggagtaat gtactttgga aaaagcgggg tttttcctgt
    10021 tgcgcatttc cactacggta ttctcctgcc gtaatggaat aacgagtatg cccttcacgt
    10081 tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtgctgcc gtcagcctct
    10141 ttaatcgtta cctgcaagtc accactatta cctgcagcat agatatcgtt gatggtaaaa
    10201 ggccccggcg gcaccgtact attataaatg tcatacccat tttgtttaat agtgacctgt
    10261 gcagtaccac gagcaatacc gtggatcacc ggagcaaatc ctctttggct atcgggtaac
    10321 atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc
    10381 tgagtataac catcacccag cgtcagccgg gaacgtaacg gtattatgtc tcgctcaagc
    10441 caggtattga tatgctgcca tttatttttg ctacctgatg atctgtcgct actgttataa
    10501 ctccaggtgg tattgtcgcg taaacgccac gcaccaatat ttaacccact ctgtaggttt
    10561 aaatatgcat aatggctgtt acccccaatc cgattctgta cactatttcc gctgaaatta
    10621 taattgagca atccggcatt aataccggga tcccataact caggaggaat ataaccacgc
    10681 gcgcgattac tcataaatgc ctgagggatc gtcaggttga gtcgctgctg accaacatct
    10741 aaatgcgcag tagcgtcctg gaccattgtg gttaatggca cacaggcatc atccgccagc
    10801 agattcatac cggcgacaga agccgtattc agccccatac tggcaagttg cgcgcgtgtc
    10861 aggcagggaa caatcccttg ttcgctgtcg cccgtattaa atgtgacatc acgcgttgcc
    10921 atataaccat tattcaaata gatatcgaca cgatacgtcc ctggcggtaa ttcttgccca
    10981 ttttcaaaac gcgataaatc ggccacagcc tggggatcat ccgctaaaaa gcgcggatta
    11041 aaatagagtt cggcagatga caaaggtgcc tgtgcggcaa aagcacaggc gacaacgagc
    11101 cggacaaaaa aaccagccaa acgatgctta cgaatatgca agcattgtgt gtttcgctgg
    11161 taaagtctta aattcagata tgacattatt cctccggttt aaaatcaccc ggcagtcaat
    11221 tctttttatt caggcgaaaa attccccctg cattattcca ttacgcccgt catttttggg
    11281 gtaagtgcgc cataatcatt tattgttcgg taagtaatat tgcttcccgc atcagaaggc
    11341 aatttaaccg tgctttcgcc cattggaggc accaaggcat tttcaagaac ccgggctccg
    11401 gcattcaact ctgttaccgt caggtaatag ggtgtcgggt taatcagcgt cagagaattc
    11461 gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg gtggcaacgc taatttagcc
    11521 ggacgatagt acagtttaat gcggctgata attgcgagct gtagcgtatt ctcagtcaat
    11581 tttgatttat ccattgacgg aatcgcttta acgttcatcc agaataaact ttcccggtcc
    11641 tgtggcaatt ggttatttgt tgcatcaaga atacgcaagg tattctcttt ttttcccttc
    11701 atcgcaaaca gaggaggcgt cacgataaaa cgaccatcct ttacaccatc ggcattttcc
    11761 acccatgatt gaattaaata ggtactattt tcatcattat ttgtcacggc aagttgcact
    11821 tgtttttgcc ctgccggata aattacgcga gtcgcaccta aggccactcc cgcttcagcg
    11881 cgtccggcaa ccatcattgc catgaacatc aggatacctg ccagcaagca gaatgttatt
    11941 tcctgcgatt tccttacatt gacgttttta ttactcactg tcctgttcct gttatcacat
    12001 tatctgctga acaattactg ataggttaaa gagaaccagg cctgtgcatt ggcgatgccg
    12061 ccagtaaccc gacgcccggt agcacgatat ttggcgatga aatgtagcga agtagagcct
    12121 gagtaaagcc gtttccagtt tgctggagga cgattaatcg gtacgaggtt tccttcatca
    12181 tcaaacaagg ctacgccaat attggtggct atccctggcc cctctcccac ggaaagcaca
    12241 tccggatttt taccatccgc gacaccgtga aacgccacac ctacacgttc actcaccacc
    12301 gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc ccctaccgca
    12361 tgaaaccggt tactgctgat ttgccccata ttgaccgtca tttgtttatc accggcttca
    12421 atccggcaag tttccgcaat aatgacgccc tggaattgca tatttccgcc gggcaacgtg
    12481 gtattccatt tatttccggc cagagcaaac attggcagca acgacgctaa tagaaataat
    12541 ctcttccttt tcatcgttat ttttatcgca caagggtggg catccctgcc cgtaatgacg
    12601 tccctgaacc tgggtaggtt attgatactg aaccttgaag gtcgcatccg cattagctgc
    12661 gccaggggtt gcggccccaa ttgcataata acgcgcctgg aacggaatgg tgttggttcc
    12721 gttattcagg gttgtttgtg cactaaatgt cgcaccgtcc agcgccagcg cagcgcccgt
    12781 tctgtccagg atctgcacac caacttttgt tgcgctaccc gcagctgaac tctgcaaagc
    12841 taatacgtta ggatgagtgc catcaattgc ggtaccaagg aaggcaatag ctgctttgtc
    12901 tgcaacagta gtgtcacaat cgttcagttg aatgttaaaa ccgacagcag aactggttgc
    12961 tccttcttgt gccagcgatg cggtacgaac ctgtcctaac tgaacggttt gatcaacaga
    13021 gcctgcatca actgcgcaag cggcattaac aacttcccct ttaaagtgaa cggtcccacc
    13081 atttaccgtc gtagtatcgg ccagagccgc tgcggaactg agggacagag ccgacagaac
    13141 aacgattgcc agagttttaa ttttcatgct gctttccttt aaaaaaacta tttctaaatc
    13201 gacatgggca gtcgttctgt acactttgtt ttgtcaacga gtttgactgc caacactgca
    13261 cagttttccc ccaaaagatg aaacatttgg ggccattttg actcatagag gaaagcatcg
    13321 cgcacaaact ttttcagttt a
//
LOCUS       DAZUGK010000120        13325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZUGK010000120 DAZUGK010000000
VERSION     DAZUGK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.121"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..866
                     /locus_tag="ACHY5W_003088"
     CDS             3..866
                     /locus_tag="ACHY5W_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFS4447951.1"
                     /translation="MSIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDL
                     LIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLE
                     LTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELVLRITSLLD
                     FLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTRE
                     ATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRV
                     QSTSKTAPQAK"
     gene            complement(1059..1412)
                     /locus_tag="ACHY5W_003089"
     CDS             complement(1059..1412)
                     /locus_tag="ACHY5W_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFS4447952.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1550..3196)
                     /gene="groL"
                     /locus_tag="ACHY5W_003090"
     CDS             complement(1550..3196)
                     /gene="groL"
                     /locus_tag="ACHY5W_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4447953.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3240..3533)
                     /locus_tag="ACHY5W_003091"
     CDS             complement(3240..3533)
                     /locus_tag="ACHY5W_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4447954.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3809..5065
                     /gene="yjeH"
                     /locus_tag="ACHY5W_003092"
     CDS             3809..5065
                     /gene="yjeH"
                     /locus_tag="ACHY5W_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFS4447955.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5081..5557)
                     /locus_tag="ACHY5W_003093"
     CDS             complement(5081..5557)
                     /locus_tag="ACHY5W_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFS4447956.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5894..7330
                     /gene="aspA"
                     /locus_tag="ACHY5W_003094"
     CDS             5894..7330
                     /gene="aspA"
                     /locus_tag="ACHY5W_003094"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFS4447957.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7448..8749
                     /gene="dcuA"
                     /locus_tag="ACHY5W_003095"
     CDS             7448..8749
                     /gene="dcuA"
                     /locus_tag="ACHY5W_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFS4447958.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8865..9203
                     /gene="cutA"
                     /locus_tag="ACHY5W_003096"
     CDS             8865..9203
                     /gene="cutA"
                     /locus_tag="ACHY5W_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFS4447959.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9179..10876
                     /gene="dsbD"
                     /locus_tag="ACHY5W_003097"
     CDS             9179..10876
                     /gene="dsbD"
                     /locus_tag="ACHY5W_003097"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFS4447960.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGAMLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTTQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10913..11488
                     /locus_tag="ACHY5W_003098"
     CDS             10913..11488
                     /locus_tag="ACHY5W_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4447961.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11595..11670
                     /locus_tag="ACHY5W_003099"
     tRNA            11595..11670
                     /locus_tag="ACHY5W_003099"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11628..11630,aa:Phe,seq:gaa)
     gene            11868..13133
                     /locus_tag="ACHY5W_003100"
     CDS             11868..13133
                     /locus_tag="ACHY5W_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4447962.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
BASE COUNT         3237 a         3437 c         3272 g         3379 t
ORIGIN      
        1 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
       61 tggcgctgaa aattaaagaa ccccgcaata aagaatcgtt gttctttatg tctgtcatgg
      121 aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg
      181 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      241 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      301 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      361 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      421 ttcatgcaat taataatgcg gaaatgcgtg aactggtatt acgtattacc tcactgttag
      481 atttcctgcc gttatatgat gttgactgcc aggaaaatgg caatctggaa tatgacacct
      541 attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg ctttaccgct
      601 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      661 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga
      721 aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc
      781 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca
      841 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
      901 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
      961 ccggcctaca aattctcaag cattcaatca gttgcaatgc cttatgtagg cctgataagc
     1021 gaagcgcatc tggcaattat acgtttgtca ccatcacatc agttcgggca cttatatacc
     1081 tggccgataa tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggcta
     1141 ctgatgccat aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct
     1201 gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt gctttgctta
     1261 cctgtcgcag taccaatcag ctggcactct gcgcctggct gctcgtccac aatgcgtaca
     1321 ctctgaccgg cagcactcaa ttcgttgctg gagctacaac ccgccatcag taacgcggca
     1381 ccgacaatcc ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac
     1441 gccactcgcg tcgtccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc
     1501 cagacatttc tgcccggggg tttgtttatt tctgcgaggt gcagggcaat tacatcatgc
     1561 cgcccatgcc acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt
     1621 tcggcaggtc ggtaaccatg cactcggtgg tgatcatcag gccagccaca gaagccgcgt
     1681 actgcagagc agaacgggtt actttggttg gatccaggat acccatgtcg atcatgttgc
     1741 cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag
     1801 caacaacaga cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg
     1861 cacgcagtgc aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca
     1921 gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg
     1981 cagcacgggt cgcgtgcagg gcgtcttcaa cgcgtgcttt tttctctttc atttcaactt
     2041 cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct
     2101 gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa
     2161 cacggccctg gattgcagct tcttcgccca cgccatcgat gatggtggtg gtgtctttgt
     2221 tgatcacaac acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac
     2281 cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt
     2341 tacgacgatc gccgaagccc ggagctttaa ctgcagcaac tttcacgatg ccacgcatgg
     2401 tgttaacaac cagagttgcc agcgcttcgc cttctacatc ttcagcgatg atcagcagcg
     2461 gtttgcctgc tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttggagattt
     2521 tcttgtcagc cagcaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt
     2581 tgatgaagta aggagacaga tagccacggt cgaactgcat accttcaacc acgtccagtt
     2641 cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca
     2701 tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagata gtaccaacct
     2761 gagcaatcgc tttagagtca gagcacggta cggacagcgc tttcagttct tcaactgcag
     2821 cggtaacagc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag
     2881 ctttcagacc ttcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc
     2941 ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc atgttttcga
     3001 acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg
     3061 caccgaaaga tttatccaga actacgttac ggcctttcgg accgagggta actttcactg
     3121 catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta
     3181 cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgaggat
     3241 tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg
     3301 atcttctcgg atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg
     3361 tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg
     3421 ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt
     3481 tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac
     3541 tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg
     3601 ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa
     3661 ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc
     3721 ttcatcgccg tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca
     3781 aaatttgggc gcaatcggac atcaacccat gagtggactc aaacaagaac tggggctggc
     3841 ccagggcatt ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc
     3901 gttagctgcg ctggtagcgg gcaataacag cctgtgggcg tggcccgttt tgattatctt
     3961 agtgttcccg attgcgattg tgtttgcgat tctgggtcgc cactatccca gtgcaggcgg
     4021 cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggct
     4081 gtttttatcg gtcattcccg tgggtttgcc tgccgcgcta caaattgctg ccggattcgg
     4141 ccaggcaatg tttggctggc atagctggca actgttgttg gcagaactcg gtacgctggc
     4201 gttggtgtgg tatatcggta ctcgcggtgc cagttccagt gcaaatctac aaacagttat
     4261 tgccggactt atcgtcgcgc tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc
     4321 gaatatcccc ttccctgcgc caggaaatat cgaacttacc gggttatttg ctgcattatc
     4381 agtgatgttc tggtgttttg tcggtcttga agcatttgcc catcttgcct cggaatttaa
     4441 aaatccagaa cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg caggattagt
     4501 ctactggggc tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc
     4561 ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt gtaggagcgt tatggattgc
     4621 ctgcgtgatt ggctatctgg cctgctttgc cagtctcaac atttatatac agagcttcgc
     4681 ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc
     4741 tcgccatatc ccgaataatg ccctcaatgc ggtgctcggc tgctgcgtgg tgagcacgtt
     4801 ggtgattcat gctttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt
     4861 tattatgatt tatctgttat gcatgctggc aggctgtaaa ttattgcaag gacgttatcg
     4921 actactggcg gtggttggcg ggctgttatg cgttctgtta ctgacaatgg tcggctggaa
     4981 aagtctctat gcgctgatta tgctggcggg gttatggctg tttctgccaa aacgaaaaac
     5041 gccggaaaat ggcataacca cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt
     5101 tatgatcaag gcggtcgcgg tcatcatcct ttcgctggta ctcaccatca aaagtattac
     5161 cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca
     5221 tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt
     5281 cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat gatcagcgaa acacttttaa
     5341 tcatctccgc cgcagggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct
     5401 taaagccctg gttacgtacc agtgacatac cgataactga cgtgaatata accagcacga
     5461 gggtcagcaa tacccccaat acatgggcaa cctgaataaa gattgaaatc tcaatataga
     5521 cataaaggaa aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc
     5581 ggttaacccc ggctacctga atgggttgcg aatcgcgttc agcttatatt gtggtcatta
     5641 gcaaaatttc aagatgtttg cgcaactatt tttggtagta atcccaaagc ggtgatctat
     5701 ttcacaaatt aataattaag gggtaaaaac cgacacttaa agtgatccag attacggtag
     5761 aaatcctcaa gcagcatatg atctcgggta ttcggtcgat gcaggggata atcgtcggtc
     5821 gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag caaatcattg gcagcttgaa
     5881 aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa gatctgttgg gtaccaggga
     5941 agttccagct gatgcctact atggtgttca cactctgaga gcgattgaaa acttctatat
     6001 cagcaacaac aaaatcagtg atattcctga atttgttcgc ggtatggtaa tggttaaaaa
     6061 agccgcagct atggcaaaca aagagctgca aaccattcct aaaagtgtag cgaatgccat
     6121 cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt tcccggtaga
     6181 cgtctaccag ggcggcgcag gtacttccgt aaacatgaac accaacgaag tgctggccaa
     6241 tatcggtctg gaactgatgg gtcaccagaa aggtgaatat cagtacctga acccgaacga
     6301 ccatgttaac aaatgtcagt ccactaacga cgcctacccg accggtttcc gtatcgcagt
     6361 ttactcttcc ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg
     6421 taaagctgtc gaattccagg acatcctgaa aatgggtcgt acccagctgc aggacgcagt
     6481 accgatgacc ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa
     6541 aaacatccaa cgtaccgctg aactgctgct ggaagttaac cttggcgcaa cagcaatcgg
     6601 tactggtctg aacacgccga aagagtactc tccgctggca gtgaaaaaac tggctgaagt
     6661 cactggcttc ccatgcgtac cggctgaaga cctgatcgaa gcgacctcag actgcggcgc
     6721 ttatgttatg gttcacggtg cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa
     6781 cgacctgcgc ttgctctctt ctggtccacg tgccggcctg aacgagatca acctgccgga
     6841 actgcaggcg ggctcttcca tcatgccagc taaagtaaac ccggttgttc cggaagtggt
     6901 taaccaagta tgcttcaaag tcatcggtaa cgacaccact gttaccatgg cagcagaagc
     6961 aggtcagctg cagttgaacg ttatggagcc ggtcattggc caggctatgt tcgaatccgt
     7021 tcacattctg accaacgctt gctacaacct gctggaaaaa tgcattaacg gcatcactgc
     7081 taacaaagaa gtgtgcgaag gttacgttta caactctatc ggtatcgtta cttacctgaa
     7141 cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa
     7201 gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact gaagcggaac ttgacgatat
     7261 tttctccgta cagaatctga tgcacccggc ttacaaagca aaacgctata ctgatgaaag
     7321 cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt
     7381 ttcttgtgag cagtaactta aaaataacaa tctaatatca acttgttaaa aaacaaggaa
     7441 ggctaatatg ctagttgtag aactcatcat agttttgctg gcgatcttct tgggcgccag
     7501 attgggggga ataggtattg gttttgcagg cggattgggg gtgctggttc ttgccgctat
     7561 tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc attatcatgg cggttatcgc
     7621 cgctatttct gccatgcagg ttgctggcgg tctggactat ctggttcatc agacagaaaa
     7681 gctgctgcgc cgtaacccga aatacatcac gatcctcgca ccgatcgtga cctatttcct
     7741 gactatcttt gctggtactg gcaacatctc tctggcgaca ctgccagtta tcgctgaagt
     7801 tgcgaaggaa caaggcgtta aaccttgccg tccgctgtct actgcagtgg tatccgcgca
     7861 gattgcgatc accgcatcgc caatctcagc ggcagtggtt tacatgtctt ccgtgatgga
     7921 aggtcatggc atcagctacc tccatctgct atccgtggtc atcccgtcca ccctgctggc
     7981 ggttctggtg atgtccttcc tggtcactat gctgttcaac tccaaactct ctgacgatcc
     8041 gatttatcgc aagcgtctgg aagagggcct ggttgaactg cgcggtgaaa agcagattga
     8101 aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt
     8161 tatctatgca atcatcaaca gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac
     8221 caccaacgca atcctgatca tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa
     8281 agtcgatacc gacaacatcc tcaactccag caccttcaaa gcaggtatga gcgcctgtat
     8341 ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca tcgactggat
     8401 caaagatacc gctggtgaag tgattcaggg tcatccgtgg ctgctggccg tcatcttctt
     8461 ctttgcttct gctctgctgt actctcaggc tgcaaccgca aaagcactga tgccgatggc
     8521 tctggcactg aacgtttctc cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct
     8581 gttcattctg ccgacctacc cgacgctggt tgctgcggta cagatggatg acacgggtac
     8641 tacccgtatc ggtaaattcg tcttcaacca tccgttcttc atcccgggta ctctgggtgt
     8701 tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat gaccaatcgc
     8761 ggggcgttca cgccccgctt tctttcccgc cgactaacat ccttcccccg tccgttgtat
     8821 agtgacctct ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa
     8881 gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc agatgaagcg acagcccagg
     8941 atttagccgc caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg atccccggcg
     9001 ctacctctct ctattactgg gaaggtaagc tggagcaaga atacgaagtg cagatgattt
     9061 taaaaactac cgtatctcac cagcaggcac tgctggaatg cctgaagtct catcatccat
     9121 atcaaacccc ggaacttctg gttttacctg ttacacacgg agacacagat tacctctcat
     9181 ggctcaacgc atctttacgc tgatcctgct actttgcagc acttccgttt ttgccggatt
     9241 attcgacgcg ccgggacgtt cacaatttgt ccccgcggat caagcctttg cttttgattt
     9301 tcaacaaaac caacatgacc tgaatctgac ctggcagatc aaagacggtt actacctcta
     9361 ccgtaaacag atccgcatta cgccggaaca cgctaaaatt gccgacgtgc agttgccgca
     9421 aggcgtctgg catgaagatg agttttacgg caaaagcgag atttaccgcg atcggctgac
     9481 gcttcccgta accatcaacc aggcaagtgc gggagcgatg ttaactgtca cctaccaggg
     9541 ctgtgctgat gccggtttct gttatccgcc agaaaccaaa accgttccgt taagcgaagt
     9601 ggtcgccaac aacgcagcgc cacagcctgt gtctgttccg cagcaagagc agcccaccgc
     9661 gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt attggtatcg cctttacgcc
     9721 atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg ctgggtggta aacagcggct
     9781 ctccactgcc agagcattgt tgctgacctt tatttatgtg caggggatgg cactgactta
     9841 cacggcgctg ggtctggtag ttgccgccgc agggttacag ttccaggcgg cgctacagca
     9901 cccatacgtg ctcattggcc tcgccatcgt ctttaccctg ctggcgatgt caatgtttgg
     9961 cttgtttact ctacaactcc cctcctcgct gcaaacgcgc ctcacgctga tgagcaatcg
    10021 ccaacagggc ggctcacccg gcggagtgtt tattatgggg gcgattgccg gactgatctg
    10081 ttcaccatgc accaccgcac cgcttagcgc gattctgctg tatatcgccc aaagcgggaa
    10141 catgtggctg ggcggcggca cgctttatct ctatgcgttg ggcatgggcc tgccgctgat
    10201 gctaattacc gtctttggta accgcttgct gccgaaaagc ggcccgtgga tggaacaagt
    10261 caaaaccgcg tttggttttg tgatcctcgc actgccggtc ttcctgctgg agcgagtgat
    10321 tggtgatata tggggattac gcttgtggtc ggcgcttggt gtcgcattct ttggctgggc
    10381 ctttatcacc agcctacagg ccaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct
    10441 tgcagcggca ttggttagcg tgcgcccact tcaggattgg gcatttggtg cgacgcatac
    10501 cacgcaaact cagacgcatc tcaactttac acaaatcaaa acggtagatg agttaaatca
    10561 ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat ctttatgccg actggtgcgt
    10621 cgcctgtaaa gagtttgaga aatacacctt cagcgacccg caggtgcaaa aagcgttagc
    10681 agacacggtc ttacttcagg ccaacgtcac ggccaacgac gcacaagatg tggcgctgtt
    10741 aaagcatctt aatgtccttg gcctaccgac aattctcttt tttgacggac aaggccagga
    10801 gcatccacaa gcacgcgtca cgggctttat ggatgctgaa accttcagcg cacatttgcg
    10861 cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg
    10921 tgaagatgta ctgggagaag ccctgaaatt attagaatta caagggattg ccaacaccac
    10981 gctggagatg gttgctgaac gtgtggatta tccactggac gagctgcgcc gcttctggcc
    11041 agacaaagag gcaatcctct acgatgcgct gcgttatctt agccaacaga tagatgtctg
    11101 gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa caaaagttgc tggcacgtta
    11161 tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctgt ttatcgctgc
    11221 ctgtacgttt tatcccgatc ctggccaccc tattcatcaa ctggccgatc agcaaaaaag
    11281 cgcggcctac gatttcaccc acgaactgtt aaccacgctg gaagttgacg atccggcgat
    11341 ggtagcaaag cagatggaac tggtactgga aggctgttta agccgaatgc tggtgaatcg
    11401 tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa gatatcttgc gcttcgcccg
    11461 ctgccgccag ggtggcgcat tgacctgaca gaaacacaga aaagaagcga tttgccgcaa
    11521 tcttaagcag ttgaatcgct tttactgaaa ttaggttgac gagatgtgca gattacggtt
    11581 taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa aatccccgtg
    11641 tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc cacggaggtc
    11701 cgtttttcgt ttcagaacac catgatttaa gtgttcttcc tccaaatcaa ttctaccgaa
    11761 ctcaaccaaa ttctccccac atcaacctca ttgtgtgggt ataattgcgg gcatacccca
    11821 gttcgacaga atttgtaccc tctttaacgc attcagaagg ctgaactatg gcactgactg
    11881 acgcaaaaat ccgggctgca aagcccactg acaaggctta taaactcact gacggggctg
    11941 gcatgttcct gctggtacat cctaatggtt cccgttactg gcgtctccgt tatcgtattc
    12001 tgggtaaaga gaagactctg gcacttggtg tgtatccaga agtatctctc tccgaagctc
    12061 gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg ggttgaccct tgcgaacaga
    12121 aaagagctaa aaaagtagtc cctgatttac aactctcttt cgaacatatt gcacgacgct
    12181 ggcatgccag taataaacaa tgggcacaat cacacagcga taaagtactc aaaagcctcg
    12241 agacacacgt attccccttt atcggcaatc gggatatcac aacactcaat accccggacc
    12301 tgcttatccc tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc agtcgtctgc
    12361 agcaaagaat atctgccgta atgcgttatg ccgtacagtc tggcatcatc agatataatc
    12421 ctgctctgga tatggctggc gcattgacta cggtaaaacg ccagcatcgc cccgctcttg
    12481 atctttctcg cctgcctgaa cttttgtcgc gtattggcag ttacaagggg caacctgtca
    12541 cccagcttgc cgttatgctg aatttactgg tttttattcg ttccagtgaa ctcagatacg
    12601 cccgctggtc tgaaattgat attgacaatg ccatgtggac tattccagcc gaacgcgaac
    12661 ctctgccagg cgtaaaattc tcacaccggg gatccaagat gcgaacacca catcttgtgc
    12721 cactcagcaa acaggttgta gccatactgg cagaacttca gacatgggct ggtgaaaatg
    12781 gtctgatctt tacgggagca catgacccac gtaaaccaat cagtgaaaac accgtaaata
    12841 aagccctgag ggttatgggg tatgacacaa cccaggatgt ctgtggtcat ggattccggg
    12901 cgatggcatg tagtgcgtta atagaatcag gtttgtggtc ccgcgatgca gttgaacgtc
    12961 agatgagcca tcaggaacgt aacggtgtac gtgctgctta cattcataaa gcagaacatc
    13021 tggaagaacg tcgactgatg ctacagtggt gggccgattt tctggatgca aacagagaaa
    13081 gatttatcag tccatttgaa tatgcaaaga ttaataatcc attaaaacag taatcatccc
    13141 gggcaaatgc ccgggaacct actccaggat tattttcttt gttaaaaaaa cagacaaaac
    13201 ggtattaact tcgtaagcgt acagcgaggg ccgtattgac ggggatgtgt tattcagctg
    13261 gcagtgctat gcgccacgga agcagttcgc tgacccggtt gaccggccag tctgctatga
    13321 cgcca
//
LOCUS       DAZUGK010000121        13183 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZUGK010000121 DAZUGK010000000
VERSION     DAZUGK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.122"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..834
                     /locus_tag="ACHY5W_003101"
     CDS             <1..834
                     /locus_tag="ACHY5W_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4447963.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHY5W_003102"
     CDS             876..1271
                     /locus_tag="ACHY5W_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4447964.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            1283..1636
                     /locus_tag="ACHY5W_003103"
     CDS             1283..1636
                     /locus_tag="ACHY5W_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4447965.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            1648..2226
                     /locus_tag="ACHY5W_003104"
     CDS             1648..2226
                     /locus_tag="ACHY5W_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4447966.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHY5W_003105"
     CDS             2223..2618
                     /locus_tag="ACHY5W_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4447967.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHY5W_003106"
     CDS             2626..3366
                     /locus_tag="ACHY5W_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4447968.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHY5W_003107"
     CDS             3382..3804
                     /locus_tag="ACHY5W_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4447969.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHY5W_003108"
     CDS             3786..4220
                     /locus_tag="ACHY5W_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4447970.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..6792
                     /locus_tag="ACHY5W_003109"
     CDS             4213..6792
                     /locus_tag="ACHY5W_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4447971.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            6789..7118
                     /locus_tag="ACHY5W_003110"
     CDS             6789..7118
                     /locus_tag="ACHY5W_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4447972.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            7118..7816
                     /locus_tag="ACHY5W_003111"
     CDS             7118..7816
                     /locus_tag="ACHY5W_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4447973.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            7822..8565
                     /locus_tag="ACHY5W_003112"
     CDS             7822..8565
                     /locus_tag="ACHY5W_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4447974.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            8562..9134
                     /locus_tag="ACHY5W_003113"
     CDS             8562..9134
                     /locus_tag="ACHY5W_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4447975.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            9195..12692
                     /locus_tag="ACHY5W_003114"
     CDS             9195..12692
                     /locus_tag="ACHY5W_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS4447976.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGITVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGDRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFLKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            12763..>13183
                     /locus_tag="ACHY5W_003115"
     CDS             12763..>13183
                     /locus_tag="ACHY5W_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4447977.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGK"
BASE COUNT         3023 a         3307 c         4193 g         2660 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggtggtat
     1081 cagcgcagcc ggataccgtg attgatacgg ctgctctggt cacggtcgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgagcc tgtggcattt gtgctgccgg
     1201 gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat gacagaacat ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga ttccgataac ctgttcgatg ctgccattgc
     1321 ccgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gctgtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggggtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgtggagaca cactgaccat cggcgaggaa aacttctggg tggaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cgtggcgtgc cgcctgccgt
     1621 taaccgtcgc cgctgaaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcaa aacggcggtg cctggtgccg ccgcaatggc cattaaccgc
     1741 gttgcgtcat ccgcgatatc gcagtctgcg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaggg ttaaccgggg ggatttgccc gtaatcaagc tgggtaacgc gcgggttgtc
     1921 ctttcccgcc gcagacgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggaaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg tcatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaga atattgaacg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgagaat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acatggcagg
     2401 cggagctgca tattgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gtgatatccc ggcactgtca gatttgatca
     2521 ccagtatggt ggccagcggc tatgactacc ggcgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg aagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgca
     2821 gactggactg cgaccgggca ggggcagaaa tctgccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cggcatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagcccgt ggctgatcct gctgcaacag
     4501 gggggtcagg ttaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcaggcctga cgtttaacca gaccagcgag tcactgacgg cgctggtgaa tgccggtgtg
     4801 cgtggtggtg agcagtttga ggcgatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 ggactgacag cgatggcacg tcagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg agatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggacagcacg ggcattcaaa tccatgtggg attcggtgct ggatattggt
     5161 cgcccggaca ctgcccaggg aatgctggag aaagcagaaa aggcttttga tgaggcggac
     5221 aaaaaatggc agtggtatca gagccggagc caccggcgcg gtaaaacctc agcatttctt
     5281 gccaatctcc ggggagcatg ggaggacaga gcgaatgcgc aacttgggct ttcagccgcc
     5341 acgttgcagg ccgatcttga aaaggccaga gagatggcag caaaggactg ggccgagtct
     5401 gaggcatcac ggctgaaata taccgaagag gcgcagaagg cttacgaacg gctgcagacg
     5461 ccgctggaga aatataccgc ccgtcaggaa gaactgaaca aggcactgaa agacgggaaa
     5521 atcctgcagg cggattacaa cacgctgatg gcggcggcga aaaaggatta tgaagcgacg
     5581 ctgaaaaagc cgaaacagtc cggcgtgaag gtgtctgcgg gcgatcgtca ggaagacagt
     5641 gctcatgctg ccctgctgac gcttcaggct gaactccgga cgctggagaa gcatgccgga
     5701 gcaaatgaga aaatcagcca gcagcgccgg gatttgtgga aggcggagag ccagttcgcg
     5761 gtactggagg aggcggcgca acgtcgccag ctgtctgcac aggagaaatc cctgctggcg
     5821 cataaagatg agacgctgga gtacaaacgc cagctggctg cacttggcga caaggttacg
     5881 tatcaggagc gcctgaacgc gctggcgcag caggcggata aattcgcaca gcagcaacgg
     5941 gcaaaacggg ccgccattga tgcgaaaagc cgggggctga ctgaccggca ggcagaacgg
     6001 gaagccacgg aacagcgcct gaaggaacag tatggcgata atccgctggc gctgaataac
     6061 gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc agcttcgcgg gagctggatg
     6121 gcaggcctga agtccggctg gagtgagtgg gaagagagcg ccacggacag tatgtcgcag
     6181 gtaaaaagtg cagccacgca gacctttgat ggtattgcac agaatatggc ggcgatgctg
     6241 accggcagtg agcagaactg gcgcagcttc acccgttccg tgctgtccat gatgacagaa
     6301 attctgctta agcaggcaat ggtggggatt gtcgggagta tcggcagcgc cattggcggg
     6361 gctgttggtg gcggcgcatc cgcgtcaggc ggtacagcca ttcaggccgc tgcggcgaaa
     6421 ttccattttg caaccggagg atttacggga accggcggca aatatgagcc agcggggatt
     6481 gttcaccgtg gtgaatttgt cttcacgaag gaggcaacca gccggattgg cgtggggaat
     6541 ctttaccggc tgatgcgcgg ctatgccacc ggcggttatg tcggtacacc gggcagcatg
     6601 gcagacagcc ggtcgcaggc gtccgggacg tttgagcaga ataaccatgt ggtgattaac
     6661 aacgacggca cgaacggtca gataggtccg gctgctctga aggcggtgta tgacatggcc
     6721 cgcaagggtg cccgtgatga aattcagaca cagatgcgtg atggtggcct gttctccgga
     6781 ggtggacgat gaaaaccttc cgctggaaag tgaaacccgg tatggatgtg gcttcggccc
     6841 cttctgtaag aaaggtgcgc tttggtgatg gctattccca gcgagcgcct gccgggctga
     6901 atgccaacct gaaaacgtac agcgtgacgc tttctgtccc ccgtgaggag gccacggtac
     6961 tggagtcgtt tctggaagag cacgggggct ggaaatcctt tctgtggacg ccgccttatg
     7021 agtggcggca gataaaggtg acctgcgcaa aatggtcgtc gcgggtcagt atgctgcgtg
     7081 ttgagttcag cgcagagttt gaacaggtgg tgaactgatg caggatatcc ggcaggaaac
     7141 actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg gtgctctggg aaatcgacct
     7201 gacagaggtc ggtggagaac gttatttttt ctgtaatgag cagaacgaaa aaggtgagcc
     7261 ggtcacctgg caggggcgac agtatcagcc gtatcccatt caggggagcg gttttgaact
     7321 gaatggcaaa ggcaccagta cgcgccccac gctgacggtt tctaacctgt acggtatggt
     7381 caccgggatg gcggaagatc tgcagagtct ggtcggcgga acggtggtcc ggcgtaaggt
     7441 ttacgcccgt tttctggatg cggtgaactt cgtcaacgga aacagtgacg ccgatccgga
     7501 gcaggaggtg atcagccgct ggcgcatcga gcagtgcagc gaactgagcg cggtgagtgc
     7561 ctcctttgta ctgtccacgc cgacggaaac ggatggcgct gtttttccgg gacgtatcat
     7621 gctggccaac acctgcacct ggacctatcg cggtgacgag tgcggttata gcggtccggc
     7681 tgtcgcggat gaatatgacc agccaacgtc cgatatcacg aaggataaat gcagcaaatg
     7741 cctgagcggt tgtaagttcc gcaataacgt cggcaacttt ggcggcttcc tttccattaa
     7801 caaactttcg cagtaaatcc catgacacag acagaatcag cgattctggc gcacgcccgg
     7861 cgatgtgcgc cagcggagtc gtgcggcttt gtggtaagca cgccggaggg ggaaagatat
     7921 ttcccctgcg tgaatatctc cggtgagccg gaggcgtatt tccgtatgtc gccggaagac
     7981 tggctgcagg cagaaatgca gggtgagatt gtggcgctgg tccacagcca ccccggtggt
     8041 ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt gccgtggtgg
     8101 ctggtctgcc gggggacgat tcataagttc cgctgtgtgc cgcatctcac cgggcggcgc
     8161 tttgagcacg gtgtgacgga ctgttacaca ctgttccggg atgcttatca tctggcgggg
     8221 attgagatgc cggactttca tcgtgaggat gactggtggc gtaacggcca gaatctctat
     8281 ctggataatc tggaggcgac ggggctgtat caggtgccgt tgtcagcggc acagccgggc
     8341 gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc acgccgcaat ttactgcggc
     8401 gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag gtacaccgac
     8461 aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc atctgccttt
     8521 acggggattt acaacgattt ggtcgccgca tcgaccttcg tgtgaaaacg ggggctgaag
     8581 ccatccgggc actggccaca cagctcccgg cgtttcgtca gaaactgagc gacggctggt
     8641 atcaggtacg gattgccggg cgggacgtca gcacgtccgg gttaacggcg cagttacatg
     8701 agactctgcc tgatggcgct gtgattcata ttgttcccag agtcgccggg gccaagtcag
     8761 gtggcgtatt ccagattgtc ctgggggctg ccgccattgc cggatcattc tttaccgccg
     8821 gagccaccct tgcagcatgg ggggcagcca ttggggccgg tggtatgacc ggcatcctgt
     8881 tttctctcgg tgccagtatg gtgctcggtg gtgtggcgca gatgctggca ccgaaagcca
     8941 gaactccccg tacacagaca acggataacg gtaagcagaa cacctatttc tcctcactgg
     9001 ataacatggt tgcccagggc aatgttctgc ctgttctgta cggggaaatg cgcgtggggt
     9061 cacgcgtggt ttctcaggag atcagcacgg cagacgaagg ggacggtggt caggttgtgg
     9121 tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc tgcgggcggt tttgtcattt
     9181 atggagcgtg aggaatgggt aaaggaagca gtaaggggca taccccgcgc gaagcgaagg
     9241 acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc catcagcgaa gggccggttg
     9301 aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccggtg ctggacactg
     9361 aggggaatac caacatctcc ggtatcacgg tggtgttccg tgccggtgag caggagcaga
     9421 caccgccgga ggggtttgaa tcctccggct ccgagacggt gctgggtacg gaagtgaaat
     9481 atgacacgcc gatcacccgc accattacgt ctgcaaacat cgaccgtctg cgctttacct
     9541 tcggtgtaca ggcactggtg gaaaccacct caaagggtga caggaatccg tcggaagtcc
     9601 gtctgctggt tcagatacaa cgtaacggtg gctgggtgac ggaaaaagac atcaccatta
     9661 agggcaaaac cacctcgcag tatctggcct cggtggtggt ggataacctg ccgccgcgcc
     9721 cgtttaatat ccggatgcgc aggatgacgc cggacagcac cacagaccag ctgcagaaca
     9781 aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
     9841 cactggtcgg cgtgcaggtg gactcggagc agttcggcag ccagcaggtg agccgtaatt
     9901 atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaatta taacccgcag acgcggcaat
     9961 acagcggtat ctgggacgga acgtttaagc cggcatacag caacaacccg gcctggtgtc
    10021 tgtgggatat gctgacccac ccgcgctacg gcatggggaa acgtcttggt gcggcggatg
    10081 tggataaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg ccggacggtt
    10141 ttggcggcac ggagccgcgc atcacctgta atgcctggct gaccacgcag cgtaaggcgt
    10201 gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacgggcaga
    10261 cgctgacgtt cgtgcaggac cgaccatcag ataaggtgtg gacctataac cgcagtaatg
    10321 tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc
    10381 ataatgccgt tgaggtgaac tggattgacc cgaacaacgg ctgggagacg gcgacagagc
    10441 ttgttgaaga tacgcaggcc attgcccgtt acggtcgtaa tgtcacgaag atggatgcct
    10501 ttggctgtac cagccggggg caggcacacc gcgccgggct gtggctgatt aaaacggaac
    10561 tgctggagac gcagaccgtg gatttcagcg tgggtgctga agggcttcgc catgtaccgg
    10621 gcgatgtcat tgaaatctgc gatgatgact atgccggtat cagcaccggt gatcgcgtgc
    10681 tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg ctgccatcct
    10741 ccggtaccac gctgataagc ctggttgacg gaagtggtaa tccggtcagc gtggaggttc
    10801 agtccgtcac cgacggactt aaggtgaaag tgaaccgggt tcctgacggc gttgcagaat
    10861 acagtgtgtg ggggctgaag ttgccgacgt tgcgtcagcg cctgttccgc tgtgtgagta
    10921 tccgtgataa cgatgacggc acgtatgcca tcactgccgt gcagcatgta ccggaaaaag
    10981 aagccatcgt ggataacggg gcgcactttg acggcgacca gagcggcacg gtgaatggtg
    11041 tcacgccgcc agcggtgcag cacctgaccg ccgaagtcac cgcagacagc ggggaatatc
    11101 aggtgctggc gcgctgggac acaccgaagg tggtgaaggg cgtgagcttc ctgctccgtc
    11161 tgaccgtaac agcggacgac ggcagtgagc ggctggtcag cacggcccgg acgacggaaa
    11221 ccacataccg cttcacacaa ctggcgttgg ggaactacag gctgacagtc cgggcggtaa
    11281 atgcgtgggg acagcagggc gatccggcat cggtatcgtt ccggattgcc gcaccggcag
    11341 cgccgtctcg gattgagctg acaccgggct attttcagat aaccgccacg ccgcatcttg
    11401 cggtttatga cccgacggta cagtttgagt tctggttctc ggaaaaacgg attgcggata
    11461 tcaggcaggt tgaaaccagc gcgcgttatc ttggtacggc actgtactgg atagccgcca
    11521 gtatcaatat caaaccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg
    11581 gcaaatcggc attcgtggag gctgtcggtc agccgagtga tgatgcatca ggctatctgg
    11641 attttttcaa aggcgagata gggaaaaccc atctggctca ggagctgtgg acgcagattg
    11701 ataacggtca gcttgcgcct gacctgactg aaatcaggac gtccataacg gatgtcagca
    11761 atgaaataac acagaccgtc aataagaaac tggaagacca gagtgcagcg atccagcaga
    11821 tacagaaggt tcaggttgat acaaataata acctgaacag catgtgggca gtgaagctgc
    11881 agcagatgca ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaacaccc
    11941 ctgacggcat gcagagtcag gtgctgctgg cagcagacag gattgcgatg attaatcctg
    12001 cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaatgaag
    12061 tgttcctgaa acgcctgacg gcccccacca ttaccagcgg cggtaatcct ccggcatttt
    12121 ccctgacatc agacgggaga ctgacggcga aaaatgcgga tatcagtggc agtgtgaatg
    12181 cgaactcagg aacgctcaac aatgtcacga ttaaccagaa ctgtacgatt aagggcatgc
    12241 tggaggcgac ccaggtcaga ggagatttcg ttaaagctgt atcaaaagcc ttcctgaaaa
    12301 aagtcggtac gtggggtaac acggaaacac caaacggtac ggttacagtc accatcagcg
    12361 atgatcataa ctttgaccgc cagattatta ttccgcccat tatttttaac ggtatagcgt
    12421 atgacgatcc ggggagcgga aataacccag gaggcacgcg atacacgggt tatggttttg
    12481 aagttcgcaa aaacggcgta ttaatcgcat ccagagaaac taaaggggcc attcccggta
    12541 gttacagtgc agttattgat atgccgagtg gcaggggaag cgtcactctg gagtttaaga
    12601 ttttccagaa aggcaatcag ggggcaggca atatcaccga ctgtacggtg attgtgacca
    12661 aaaaagccgc ttccggcatc agtattcgtt gaaatattta taaccccaat aaagggcgtc
    12721 aggaatgacg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa agtttgtgca
    12781 gcaattttgt ccgcagccat ttgtctggcc gtatccggtg cgcctgcatg ggcgtctgaa
    12841 catcagtcca cgctgagcgc ggggtatctt catgcctcga cgaacgtccc cggcagcgat
    12901 gatctgaacg ggattaacgt gaaataccgt tatgagttta cggacacact ggggctagtg
    12961 acgtcattca gctatgcagg agacaagaat cgccagctga cccgttacag cgatacccgc
    13021 tggcatgaag attccgttcg taaccgctgg ttcagcgtaa tggcggggcc gtctgtgcgc
    13081 gtgaatgaat ggttcagcgc gtatgcgatg gcgggtgtag cttacagccg tgtgtcgact
    13141 ttctccgggg attatcttcg cgtaactgac aacaagggga aaa
//
LOCUS       DAZUGK010000122        13163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZUGK010000122 DAZUGK010000000
VERSION     DAZUGK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.123"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            294..4754
                     /gene="gltB"
                     /locus_tag="ACHY5W_003116"
     CDS             294..4754
                     /gene="gltB"
                     /locus_tag="ACHY5W_003116"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFS4447978.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSSFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4767..6185
                     /gene="gltD"
                     /locus_tag="ACHY5W_003117"
     CDS             4767..6185
                     /gene="gltD"
                     /locus_tag="ACHY5W_003117"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFS4447979.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6369..7496
                     /gene="yhcG"
                     /locus_tag="ACHY5W_003118"
     CDS             6369..7496
                     /gene="yhcG"
                     /locus_tag="ACHY5W_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFS4447980.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLISVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYE
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(7556..8020)
                     /gene="nanQ"
                     /locus_tag="ACHY5W_003119"
     CDS             complement(7556..8020)
                     /gene="nanQ"
                     /locus_tag="ACHY5W_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFS4447981.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(8017..8892)
                     /gene="nanK"
                     /locus_tag="ACHY5W_003120"
     CDS             complement(8017..8892)
                     /gene="nanK"
                     /locus_tag="ACHY5W_003120"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFS4447982.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALCDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(8889..9578)
                     /gene="nanE"
                     /locus_tag="ACHY5W_003121"
     CDS             complement(8889..9578)
                     /gene="nanE"
                     /locus_tag="ACHY5W_003121"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4447983.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9626..11116)
                     /gene="nanT"
                     /locus_tag="ACHY5W_003122"
     CDS             complement(9626..11116)
                     /gene="nanT"
                     /locus_tag="ACHY5W_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFS4447984.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11225..12118)
                     /gene="nanA"
                     /locus_tag="ACHY5W_003123"
     CDS             complement(11225..12118)
                     /gene="nanA"
                     /locus_tag="ACHY5W_003123"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS4447985.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12240..13031)
                     /gene="nanR"
                     /locus_tag="ACHY5W_003124"
     CDS             complement(12240..13031)
                     /gene="nanR"
                     /locus_tag="ACHY5W_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFS4447986.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3131 a         3609 c         3549 g         2874 t
ORIGIN      
        1 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
       61 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
      121 tatgcagtaa ttgagatccc ctcttcaccg tattaatcga tgcgaaaagg acaacaaggg
      181 ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt tctgtgcccg
      241 tgcgcagcgg ttcggtagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
      301 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
      361 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
      421 gcgcgattct cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg
      481 atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa aactacgctg
      541 tcgggatgct cttcctgaat aaagatcctg aactcgctgc tgccgcacgc cgcatcgttg
      601 aagaagagct gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg
      661 aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga
      721 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
      781 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg
      841 tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg
      901 cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg
      961 taccgcgctg gccgctggcg caaccgttcc gctacctggc gcataacggt gaaatcaaca
     1021 ccatcaccgg taaccgccaa tgggcgcgag cgcgtactta taaattccag acaccgctta
     1081 tccctgacct gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga
     1141 tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc gccatgcgtc
     1201 tattagtacc acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
     1261 ttgactttaa ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtctg
     1321 acggtcgttt tgctgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca
     1381 tcaccaaaga taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg
     1441 acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc gacactcgca
     1501 gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc cacccttata
     1561 aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag
     1621 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
     1681 acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg
     1741 tcggttcgat gggcgacgac accccattcg ccgtgctctc cagtcagccg cgcattattt
     1801 acgactactt ccgccagcag tttgcccagg tgactaaccc accaatcgac ccgctgcgtg
     1861 aagcgcatgt tatgtcgctc gccaccagca ttggtcgtga aatgaacgtc ttctgtgaag
     1921 cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tcggatttca
     1981 aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg
     2041 acgtcaccaa aaccacgctc gaagcgacgg tcaaagagct gtgcgacaaa gccgaaaaaa
     2101 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
     2161 tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
     2221 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccatttcg
     2281 ccgtgttgct gggcttcggt gcgacggcta tttatccgta ccttgcctat gaaacgctgg
     2341 gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg ctcaactacc
     2401 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
     2461 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct gcacgatgat gtcgtgggcc
     2521 tgtgcttcca gggggcggtc agccgcattg gcggggcgag ctttgaagac ttccagcagg
     2581 atctgctgaa cctgtcgaaa cgtgcctggc tggcgcgtaa acccatcagc cagggcggcc
     2641 tgctgaaata tgtgcacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc
     2701 tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta
     2761 atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg
     2821 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga
     2881 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca
     2941 tcggcggtaa ctcgaactcc ggtgaaggcg gtgaagatcc ggcgcgctac ggcaccaaca
     3001 aagtgtcgcg catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg
     3061 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc
     3121 agttgccggg cgacaaagtc actccttaca ttgccaaact gcgctattcg gtgccgggcg
     3181 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac ttagcgcagc
     3241 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
     3301 aaccgggcgt tggcaccatc gccaccggtg tggcgaaagc ttatgccgat ttaatcacca
     3361 tcgcaggcta tgacggtggc accggcgcaa gtccgctttc atcggtgaaa tacgcaggct
     3421 gtccgtggga gctgggactt gttgaaacac aacaggcgct ggttgccaac ggcttgcgtc
     3481 ataaaatccg tttgcaggtc gatggcggcc tgaaaaccgg cgttgatata atcaaagcgg
     3541 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta
     3601 aatatctgcg tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca
     3661 aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta
     3721 tcgcccgtga aacccgcgag ctgatggcac agctgggggt aacgcggctg gtggatctga
     3781 ttggtcgcac cgacctgctg aaagagctgg acgggttcac cgccaaacag cagaaactgg
     3841 cgctgtcgaa gctgctggag actgccgagc cacatgcagg taaggcactc tactgtaccg
     3901 aaaacaaccc gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac
     3961 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac accgaccgtt
     4021 ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag
     4081 ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtgtggaacg
     4141 cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg
     4201 cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
     4261 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
     4321 gcgcgggtga acgtttcggc gtgcgtaact ccggtgcaat caccgtggta gaaggcattg
     4381 gcgacaacgg ctgtgaatat atgacgggtg gtatcgtctg cattctgggt aaaaccggcg
     4441 ttaacttcgg tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggcgatt
     4501 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
     4561 atgaagagca tctgcgtggt cttatcaccg agcatgttca gcataccggt tctcagcgtg
     4621 gtgaagagat tctggcgaat tggtcaagct tcgccactaa atttgcgctg gttaaaccga
     4681 agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagct gagttgcgcg
     4741 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
     4801 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
     4861 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
     4921 acccatactg cgagtggaaa tgcccggtac acaactacat cccgaactgg cttaagctcg
     4981 ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac actctgccgg
     5041 aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc accctgaacg
     5101 atgagtttgg cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg
     5161 aaatgggctg gcgtccggat atgtccggcg tgaaacagac cggtaaaaaa gtggcgatta
     5221 tcggcgcagg tccggcaggt ctggcgtgtg cagatgtcct gacgcgcaac ggcgtacaag
     5281 cggtcgtctt cgaccgtcac ccggaaattg gcggcttgct gaccttcggt attccggctt
     5341 tcaaactgga aaaagaggta atgacgcgcc gccgtgaaat cttcaccggc atgggtattg
     5401 aattcaaact caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt
     5461 acgatgccgt gttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg
     5521 aagacgctga tggcgtgtac gcagcgctgc cgttcctcat cgccaacacc aaacagttaa
     5581 tgggctttgg cgaaactcgt gaagaaccgt tcgtcagcat ggaaggtaaa cgcgtggtag
     5641 tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc cagggagcaa
     5701 agcacgtaac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag
     5761 tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagccg ctgggtatag
     5821 aagtaaatgg caacggcaaa gtcagcggtg tgaaaatggt gcgtaccgaa atgggcgaac
     5881 cggatgccaa aggccgtcgc cgcgcggaga ttgtggcagg ttccgaacat atcgttccgg
     5941 cagatgcggt gatcatggcg tttggttttc gcccacacag catggaatgg ctggcgaaac
     6001 acagcgtcga actggattcg caaggccgca tcatcgctcc ggaaggcagc gacaatgctt
     6061 tccagaccag caacccgaaa atctttgccg gcggcgatat cgtccgcggt tccgatctgg
     6121 tggtgaccgc tattgccgaa ggtcgtaaag cggcagacgg cattatgaac tggctggaag
     6181 tttaagctct gttccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc
     6241 gttctggaaa gcgcctcgca taatctgaag aaaaccaatg taatacgcac atctggattg
     6301 ttaccggcag atgcccggcg tatagctgat tccattattc aacactgcgc catattaagg
     6361 gacagataat ggaatcgctc tcagaaggaa ccacagcagg ctaccagcaa atccacgacg
     6421 gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt aacgcgctga
     6481 tgaccgcgac atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg
     6541 ccagggctgc gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt
     6601 ataagcgtgg gttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc
     6661 aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga
     6721 caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc aaatctttcc
     6781 ccctgccctg gtccacctac gtccgtttaa tttccgttaa aaacgctgac gcccgcagct
     6841 tttatgaaaa agagacgctc cgctgtggct ggtctgttcg ccagctagaa cggcaaattg
     6901 cgacccaatt ttatgagcga acactgctgt cacatgacaa atcagccatg ctgcaacaac
     6961 acgctcctgc cgagacgcat attcttccgc aacaggcgat acgcgatccc tttgtgctcg
     7021 aatttctgga attgaaagat gaatactcag aatccgattt tgaggaggcg ctgatcaacc
     7081 acctgatgga tttcatgctg gaacttgggg atgattttgc ctttgttggt cggcagcgaa
     7141 ggttacgcat tgatgacaac tggtttcgtg tcgatctgct gtttttccac cgccgtttac
     7201 gctgcctgct aatcgtcgat ctaaaagtgg gcaaattcag ctatagcgat gccggacaga
     7261 tgaatatgta tctcaactac gccaaagagc actggacgct accggatgaa aatccgccca
     7321 tcggtctggt tctctgtgca gagaaaggag tcggagaagc gcattatgaa ctggctggtt
     7381 tgcctaacac cgttctggcg agtgaatata agatgcaact acctgatgaa aaacgactcg
     7441 cggatgaact cgttcgaaca caggcggtgc tagaagaagg ctatagactc cgttaatttc
     7501 agcggtttcg aattctgcag acactgtctg cagaatcttt aaaacgaact aagttttaag
     7561 ccattaaatc agccttaacc ttcaccacaa cctttttaat ctcaccaggt tcgccgacaa
     7621 cgcatcctgg tttatgcggt tctcccggca taaacacggc gaacattccc ggttttaagt
     7681 tgatggtttg ctcgttttca atggcgctgc aaagctggta atcatcttca tggtggaact
     7741 cttcacactg gcgcgcagtg cctgccgtgc caaacagaat ccgttcctca ccttttaata
     7801 acagctggat atcaatgtat tgctcgtgca attccgcttt tttctcgacg ggcgattgag
     7861 tgttaaacgt catgacattc ataaaaatat tgtcgccctg taattcgtaa cgacccggcg
     7921 ctttttcttg cggtctggca gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta
     7981 acccagcaga cggtaatgac tgtacttcac ccatcatcat aatatttctc cctgggccaa
     8041 cagcgcagcc ccaagtaaac ctgcatcatg gcggtagtgc gccgccagta aatcaacatg
     8101 aaatgccgct ggctcctgcg ccagatacgt ttccaccagc gccagatacc cttctgccag
     8161 accaacgctg ccaccgacca ccacgcactg acaatcagtt gtggctttaa tatcagcgat
     8221 cagccttgcc agcacatgtg cggagcggtg aatcagctgc tgcgcctgct cgtcgccctg
     8281 cccggcgcgc atgaaaatag ttttcgcatc cgcgccagcc aactccccct gcgctgccgc
     8341 tgcaataccg cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac
     8401 tgggccgtgt ggatcggcaa gcgtatgccc gatgtgcccc gccagaccgc cagggccggt
     8461 aagcagtttg ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaagac
     8521 catatcggtt atatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc
     8581 gttaatggca atggtcggca aatcggtaag ttgctccagc gttttgacta acggaaagtg
     8641 tagcaatcca ccaagattat gcggattcag cgccagcaaa ctgccgtcgc gaataattcc
     8701 ggttgaagcg atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa
     8761 ggcatcacac aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg
     8821 gatctgcccg tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc
     8881 cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca
     8941 agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatcagcc
     9001 gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc gacatccggc gtcgctcaac
     9061 cttttcacca gcgtcagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc
     9121 gtagtgccaa taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag
     9181 cagtcggtca tcgccagtaa accgtgatga tgaatacgtg ccagcagcgt ttcaacaggc
     9241 accggacgcg ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc
     9301 gcatcgacat cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc
     9361 acaatcccga taatcggcac gctcaccacc gcacgcgtgg cttgcaaatt tgccacacct
     9421 tcgatgcgaa tggcaaccgc gcccgcctgc tctgccgcta atgccatggc tgcgacgatt
     9481 tcgggtttat cgagcgggct gtcaggaacc ggctggcagg agacaatcag gccaccgtta
     9541 gcagcgattt tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc
     9601 ccggtacata gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc
     9661 tgccaaatgg cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg
     9721 cttccgggcg cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca
     9781 ccacaaatgt cagactgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag
     9841 cgatcaacgc gccgatgatt ggggccagtg caccgcccaa tgcgccaacg ttgtaggtaa
     9901 agcccaggcc cgctgcacgc tggtcggtat cgaaataacc gccaatcagt tttggtaaga
     9961 tcccggcgat cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga
    10021 cgtttgcgcc gccaatcgca aataccggaa taatcagcag ctgcgaggcc aacagactac
    10081 aaacgtacgc tttgcgggtt cccagccagt caccgaggaa gccacctacg cagcacccca
    10141 ccgccgcgcc aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca
    10201 gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa
    10261 acaacacgac caccatcagc attacgccag ttggccagcg tttgcctgta ctctgcacca
    10321 taaagctgat aaagattgcg gcgcataaca gcccaagaac agcgacgatc gcggcatttt
    10381 gcaggttacc ggcgaagcag aaccacagcg cagtagccgc cgccagtgtc attacgatat
    10441 tggcaatgcg atgttcacca cggtagagaa tatccaccat tgtgcgtact ggtgctttac
    10501 ctgcgtgttt ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa
    10561 agatgattgg caaaatgccg ataaagaaca gcgcacgcca gccccagacc ggaaccacca
    10621 ggctgtagac ctgagcggca acgacggccc ctacagagaa gcctgaaatc agaaagccac
    10681 tagctttgtt acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt
    10741 attcacccgc catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg
    10801 gcgcaaagcc gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca
    10861 gacgacgccc gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga
    10921 taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccttgta
    10981 cttcggtgag taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc
    11041 aggcagcgga aaatgcccgc cattgtgcac gattgagatg gcgataccac gggatattct
    11101 gggttgtagt actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga
    11161 aaattgtagg gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa
    11221 caactcaccc gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt
    11281 tttcatctac cggaccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    11341 gcagaacagt tttcaggccg cggaatacac cagttttgat cagtaaatca atgactttgt
    11401 tgcattcagt ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttaacga
    11461 tcccctgata gcgccagccc atgatgttgt aggtactacc gataccacca tcagcgcccg
    11521 ccagcagacc agaggcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt
    11581 cacgacggat ctgctccatc tgatagagat cgccagaggt ctgtttcagc gcacctacgc
    11641 caggcaatgt aacaagtgtg ttgatctgat ccagggtcag ttttacccca ctcagggctg
    11701 gaatgttgta caccaccatc ggcaaaccat ccgccgaatc aataattgcc cgatagtgat
    11761 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    11821 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    11881 cgtgggcgat gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt
    11941 cacgctcgga aaggctttgt acaaaagctt cgccggtcga accacccacg tataaaccgt
    12001 caatgccctg ctgaatattg aactgaacca ggcggcgcag actcgcttta tccagtgctt
    12061 gttgttgatc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    12121 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    12181 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagttgcat
    12241 tatttctttt tgttggtggt ctgaccgaag gcgtgccagg tagcagagac gctgttgaga
    12301 tgcgattgca acgcacgatc ggcttcatcg ggatcgtggc ggcggatcgc gtcaacgatc
    12361 gcaatatgct gttgataact aacgttgtta tgttcgtgca gtgcctgatc ggtaaccgtt
    12421 gggcgtgcgg caataagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    12481 ccggggatct ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg
    12541 ttatccagcg actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    12601 gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    12661 aaatgggcaa tcccaccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    12721 atgatggtgt ccgcagaagg acgcgagacg cgggcacgtt cgccgttgtt tatttgcacc
    12781 agacctttgc gttttaacgc tgccagcgct tcacgcaccg aaggacgccc gacgttaaag
    12841 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    12901 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    12961 ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc
    13021 ataaggccca ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga
    13081 ttgaaagtag gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac
    13141 ggaataatta actattgcga aaa
//
LOCUS       DAZUGK010000123        13141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZUGK010000123 DAZUGK010000000
VERSION     DAZUGK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.124"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(166..618)
                     /gene="yafP"
                     /locus_tag="ACHY5W_003125"
     CDS             complement(166..618)
                     /gene="yafP"
                     /locus_tag="ACHY5W_003125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4447987.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(615..1670)
                     /gene="dinB"
                     /locus_tag="ACHY5W_003126"
     CDS             complement(615..1670)
                     /gene="dinB"
                     /locus_tag="ACHY5W_003126"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4447988.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1741..2526)
                     /gene="lafU"
                     /locus_tag="ACHY5W_003127"
     CDS             complement(1741..2526)
                     /gene="lafU"
                     /locus_tag="ACHY5W_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFS4447989.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2498..4210
                     /locus_tag="ACHY5W_003128"
                     /pseudo
     CDS             2498..4210
                     /locus_tag="ACHY5W_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            4315..4593
                     /locus_tag="ACHY5W_003129"
     CDS             4315..4593
                     /locus_tag="ACHY5W_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4447990.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAAGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            4586..4942
                     /locus_tag="ACHY5W_003130"
     CDS             4586..4942
                     /locus_tag="ACHY5W_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4447991.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(4999..5748)
                     /locus_tag="ACHY5W_003131"
     CDS             complement(4999..5748)
                     /locus_tag="ACHY5W_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4447992.1"
                     /translation="MYLFSHDRLLRLGVFSLPLLLPLLASAATPHISLSYAARQRMQN
                     RARVLKLYQAHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPGPLKLSDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYYRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            6061..6801
                     /gene="dpaA"
                     /locus_tag="ACHY5W_003132"
     CDS             6061..6801
                     /gene="dpaA"
                     /locus_tag="ACHY5W_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFS4447993.1"
                     /translation="MRKIALILAMLLVPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6772..7539)
                     /gene="yafJ"
                     /locus_tag="ACHY5W_003133"
     CDS             complement(6772..7539)
                     /gene="yafJ"
                     /locus_tag="ACHY5W_003133"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFS4447994.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7745..8323)
                     /gene="lpcA"
                     /locus_tag="ACHY5W_003134"
     CDS             complement(7745..8323)
                     /gene="lpcA"
                     /locus_tag="ACHY5W_003134"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFS4447995.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8563..11007
                     /gene="fadE"
                     /locus_tag="ACHY5W_003135"
     CDS             8563..11007
                     /gene="fadE"
                     /locus_tag="ACHY5W_003135"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFS4447996.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11050..11496)
                     /gene="ivy"
                     /locus_tag="ACHY5W_003136"
     CDS             complement(11050..11496)
                     /gene="ivy"
                     /locus_tag="ACHY5W_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFS4447997.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11677..12447
                     /gene="yafV"
                     /locus_tag="ACHY5W_003137"
     CDS             11677..12447
                     /gene="yafV"
                     /locus_tag="ACHY5W_003137"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFS4447998.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHRFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
     gene            12428..12634
                     /locus_tag="ACHY5W_003138"
     CDS             12428..12634
                     /locus_tag="ACHY5W_003138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4447999.1"
                     /translation="MSLGCGEWGLCLRTGRVGETRTQNQDTQALAKEINQRLDGLLSR
                     WLLLKNTGQDMATDNSTVPVLPTH"
     gene            12652..13131
                     /locus_tag="ACHY5W_003139"
     CDS             12652..13131
                     /locus_tag="ACHY5W_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS4448000.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGVNEPYYK
                     VKLTNARISNIGLTVPHTINDSPGQPEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         3112 a         3372 c         3520 g         3137 t
ORIGIN      
        1 tcaggaaatg gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc
       61 aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt ggaataagaa
      121 aacgcgcaca tattaggcgg gcgttatttt cattgcaagc tggatttaat gttgcggttt
      181 ataacgcata taaaaattaa taaaccacgc gccccggcat tcaacgcgct gctgcttaac
      241 tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgcttgcgt ccaccgtaag
      301 ttcggattca gacttaatca aaggttttaa caaagcgctg gcaaccccac ggcgggtgta
      361 ttcagggtca acaaataaca tatcgatata atgttcaatg cgggaaataa aaccaaccgg
      421 ttgtgcatta atgactgcaa cccgcacttg tgatttcgcg agtttctcct tccagcgaga
      481 ttcgtcaatc tgcgcccagg cggcaatttg ttgtggtgaa taatgctgac tggcggtcat
      541 cgtaaccgct ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct gatagtttct
      601 tatttgtatg ttattcataa tcccagcacc agttgtcttt ccatttgcgg atccagcaac
      661 gtcacatgca gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta
      721 cgcgcggtgg cgattagatc agctttattc agccgcggcc agacgtgctc ctgggtggtt
      781 tgctgaaaat cgtcgaactt taatttcacc ccctggcgag caatcagtaa atcaggtttc
      841 acctttgcca gacggcgttc aagttccgga tacagccgct cgataatcgc ctcacattca
      901 gaccagtgat gaatatcttc cgccatcgtg cgttccacgc cgacggattt tcgcaaccgt
      961 tcgctgttaa catcgcgttc gtcaatcccc tgactacgct cccacaaaat gcggccaaat
     1021 ttgccaaagc gtttaagcag tatcaccaga tcacactttt gtacatcacc gcaggtccgc
     1081 agccccatcg cttccagttt tgccgccgag actttgccga cgccggggat ttttgccagt
     1141 ggtaaggttt gtaaaaatgc cggaacttct gccggcgtaa tcacaaactg gccgttgggt
     1201 ttattcatgt cggaggcgat tttggcgaga aactttactg gtgccacgcc cgcagatgcc
     1261 gtcagttgca gctcgttgaa gatcgtctgg cggatttcct gggcgatgag ggtcgcagaa
     1321 ccgtggcaat ggacgctatc ggtgacatcg agataagcct catccagtga caacggttca
     1381 atacgcgagg tgtagcgcga gaagatttca cggatatgat ttgaggcttc tttgtaggcg
     1441 tcaaagcgcc ccggaagcaa ggtgagatgt gggcataatt tgagcgccat ccctgtcggc
     1501 atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccga
     1561 cgttcgcggc taccgccaat agcaataggg atatcgcgca gggcgggatt gtcgcgcatc
     1621 tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat tgctcacctc
     1681 tcaacactgg taaagtatac agtgatttca gggtttgaga aatgcgtaaa gattcagcat
     1741 ttagcgcatt cgctgcgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac
     1801 cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag
     1861 caccataatc tcaatacgcc gattgcccgc gctttgcgga tttttggcat ccagcagcat
     1921 ctggtccgcc attgcgctta cctgcatcac tttatcttcc ggcattccgg cctcttccag
     1981 cacccgacga gccgaaagcg cgcggtcacc cgaaaggttc cagttgttgt agatattgtt
     2041 tttgtaggcc atcgcatcgg tatgcccggt aataataatt ttattatcga gcgagtcgaa
     2101 cactggcgct aactccacca gcagcgtttt aaagaacggc ataatctgcg cgctgccgcg
     2161 ttcgaacata ttgcgattct ggtcgtcttt aatcagcacg cgtaatccct gcgggacaat
     2221 ctccatctcc agattcgctt ccatatgcgc atcacgggca atggtgttga tactggttgc
     2281 cagctcacca agctcggtgg cggatttctt cttcagcagc gccgttttct cgttgacgtc
     2341 gcgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg gcggatgaga
     2401 cgggctgatt ttatttaacg gcgacaaccc gccaccgttg aaaatcgact gcccgtgcag
     2461 cgcggcaata atactttcgc gctcggattt actgacgctg ttgacaatca actttatcgt
     2521 cgtcaccaaa ggggccgagc gtatttccga ggtttctgcc cgctttaccc tggacgcgat
     2581 gcccggcaag cagatggcga ttgacgccga tcttaacgcc gggttgatca accaggcgca
     2641 ggcgcaaacc cggcgtaaag atgttgccag cgaggccgat ttctacggcg caatggacgg
     2701 ggcatctaag tttgtgcgcg gggacgccat cgccgggatg atgattctgg cgatcaacct
     2761 gatcggcggc gtctgtatcg ggatcttcaa atacaacctg agcgccgatg ccgccttcca
     2821 gcagtatgtg ctgatgacca tcggcgacgg cctggtggcg cagatccctt ccctcctgct
     2881 ctccaccgcg gcggcgataa tcgtcacccg cgtcagcgat aacggcgata tcgcccatga
     2941 cgtgcgccac cagctgctgg caagcccgac ggtactctac accgccactg ggatcatgtt
     3001 cgtgctggca gtggtgccgg ggatgccgca cctgccgttt ttgctgttca gcgccctact
     3061 gggttttacc ggctggcgga tgagcaaacg cccgcaggcg gcggaggcgg aagagaaaag
     3121 cctcgaaacg ctgacccgca ctatcactga aaccagcgag caacaggtca gttgggaaac
     3181 cattccgctt atcgagccta tcagtttaag ccttggctac aagctagtag cgctggtcga
     3241 taaagcgcag ggcaacccac tcacccagcg gattcgcggc gtgaggcagg tgatttctga
     3301 cggcaacggc gtgctgctgc cggagatccg cattcgggaa aatttccgcc tcaagcccag
     3361 ccagtacgct attttcatca acggcattaa ggccgatgaa gcggatattc cggcggataa
     3421 actgatggcc ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga
     3481 cccggcgtac gggatgccgg tgacctggat ccagcccgcg cagaaagcga aggcgctgaa
     3541 tatggggtat caggtaatcg acagcgccag cgtgattgcc acccatgtaa acaagattgt
     3601 gcgcagctat attcctgatt tgtttaacta tgatgacatc acacagttgc ataaccgcct
     3661 gtcgtcgatg gcaccgcgcc tggcggagga tttgagcgcg gcgctgaatt acagccagtt
     3721 gctgaaagtg taccgtgcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat
     3781 cgccaccgtg ctggtcgcca gtagcacggt gactaaagat cacattctgc tggcagccga
     3841 tgtacgcctg gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagcttac
     3901 ggtgtatatg ctgaataatg agctggaaaa tctgctgacc aacgtggtga atcaggcgca
     3961 acagggcggc aaagtgatgc tcgacagcgt gccggtcgac ccgaatatgc tcaaccagtt
     4021 ccagagcacg atgccgcagg taaaagagca gatgaaagcg gcggggaaag acccggtact
     4081 gctggtgccg ccgcagctgc gccctttgct ggctcgctat gcaaggttgt ttgcgccggg
     4141 gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc
     4201 agtgagttga tggggattct tttccatgct ttgcatgcat ctgacaccat tgattgacgt
     4261 cacattgcaa acatactacg atgacaccat acaatgatgt catggattga tagtatgatt
     4321 aaggtcagcg cgctaggctc ccgccataga aaaacattcg acgatgtaat gacggagccg
     4381 ccaaaatctg gaattaaatg ggatgatgtt gttgcgctca ttaaagcggc gggtggaaca
     4441 ataaaaaata acaatgggtc cagaaggaaa tttcagattg gaacgaccaa attcagtaca
     4501 catgagccac atccaaagaa tgtgatggat aaaggcgcag tagcaggtct taaggagtgg
     4561 tttgttaact gtgtaggggt agattatgaa taaagcatcg aacattatca caattaaagg
     4621 tcaaccagcc gcagtgacat ttgaagccga tatcaatgca tttcgcggca aattcctcaa
     4681 tgtaaacggt tattgtgact ttgtagccac aagcattgag gcattatatc gtgagggcga
     4741 aagcgcgctg gatgagtgga tggcggattg tgaagaggac ggtattgccc cttttcgtga
     4801 agaagaagag caaaaacgcc tgacgcttcg tgttcctcat cacattgatt cgcggctgac
     4861 catcgtcgcc agacaacatt ccatttcaaa aaaccagctc attgttgatg tcttagaacg
     4921 tgagttttca gcccagatgt aaacaagtcg gatgccgtgt ggcatccggc cttttgccag
     4981 ttcgtcaata ttacccattt acaaaatcgt ctcttccgtc aaaatcctgc gcgcgcccaa
     5041 aaaatggtcc tgccagaaag gttcagctaa ctggcttacc cgaatggttt ccccggtgcg
     5101 tggcgactcg ataaattgcc catcgcccag atacacgccc atatgatcgg caatttcgcg
     5161 gctgtggata tggaaaaaca gcaaatctcc ccggcgcagg tcgttgttcg ccacaattgt
     5221 tgcccgatag tagtgataca tctcattggc cgtgcgcggg agcttcgcct cgaggatctt
     5281 gttgtaggca taaaaaacca acccactaca gtcgaagcct ttatcgggcc gcgtgccgcc
     5341 ccagacgtaa ggcttgccaa gctgctgctc caggcggtgg atcgccactt cggtaatatt
     5401 atgcaggtga tcgctgctta aaaagtgatt gttttccgcc agcgcctgcc agcgtcggtc
     5461 gctcaatttc agcgggccag gaaaccactc cgggtgctgt tttatctgct cacggttgtg
     5521 ctggcgcagc gcccgtctgc tttcggcatt gccttccaca atatagctgg cctgtttttt
     5581 cagatgtgcc tggtacagtt tgagtacccg cgcacggttt tgcatccgct ggcgggcggc
     5641 gtagctgagg gagatgtgcg gtgtggcggc gctggcgagt aaaggaagca aaaggggcaa
     5701 agaaaacacg cccaggcgca gcaatctgtc gtgagagaac aaatacatgg aagatataaa
     5761 ccggctgaat gaagatgaga atattagggg acagaaagga gaagcgggat tattaattgc
     5821 ttatttgcat attactatcg aggtttcata atcttaggaa ataagaaata aatccgtttt
     5881 acatgcgctt ttcggacttt tgaaaatcgc tacttatgct aaatttgtaa accctgctcc
     5941 gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc agactattcc
     6001 gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt
     6061 atgcgtaaaa tcgcattaat tcttgcgatg ttgttggtcc cgtgcgtttc atttgccggt
     6121 ttgctgggca gcagtagctc taccacgcca gtgagcaaag agtataagca gcagttgatg
     6181 ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa
     6241 atgggcgagc aatatcagct gctcgacagc tataaaatct gtaaatattc cggcgggtta
     6301 ggtccgaaac aacgtcaggg cgatttcaaa agtccggaag ggttttatag tgtccagcgt
     6361 aatcagttaa aaccagatag ccgttactac aaagcgatta atattggttt tcctaacgcc
     6421 tatgaccgtg cgcatggtta tgaagggaaa tacctgatga tccacggcga ttgtgtttcc
     6481 atcggctgct acgcgatgac caatcagggc attgacgaga tcttccagtt cgttactggc
     6541 gcgctggtat ttggtcagcc gagcgtgcag gtgagcattt acccgttccg catgaccgac
     6601 gctaatatga agcgccataa atattccaac tttaaggact tctgggagca actgaagccg
     6661 ggttacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt caatggtcgt
     6721 tacgtggtca gcaagccgtt aagccacgaa gttgtgcaac cacaactggc atcaaactac
     6781 acgctccccg aggcaaaata agcgccattc gcctggcata atcttttgcc aggtttcatt
     6841 gcccgtcagc ggctgtgttg caatcaccgt gaccacatca ttcggtgtgg tctgcgagct
     6901 gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg cgcggcgggt
     6961 gatccagtgt aaattagtcg agcaatacgc cattacatag cgaccgtccg aaagcagcat
     7021 gttgaaaacg cccttcgccc gcagttcatc cgccagtgag gcgatatatt taaacactgc
     7081 cgccatgttg cccggcgtgc gcgggtaacg ctgcgttaat ttatgcagga gccagcaaaa
     7141 ggctttttcg ctgtcggttt caccgaccgg gcggaagttg ccggtttcca gtgatttgta
     7201 gcccgtcagt tgcccgttat gggcataagt ccagttacgc ccccataact cgcgggtaaa
     7261 cgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat gagccaccac
     7321 cgaacaggat tttatcgggt agtcctggac cagtttggcg ataggtgagt taaagctggg
     7381 ttgcggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc cccagccatc
     7441 tttatgtggc ccggttccac caccacgctg caccagaccg gtgaaactaa agcagatatc
     7501 ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg
     7561 gagcgcatgg cccccggcga agattgattt aagaattttc aggtcggatg cggcgtgaac
     7621 gcattattcg gcctacggtt cgcgtacaga tttgtaggca tgataagacg cgtcagcgtc
     7681 gcatcaggca tcggtgcaca attgccggat gcggcgtaaa cgtcttatcc ggcctacgcc
     7741 agacttactt aaccatctct ttttcaatca actggatcag gatatggatc actttaatgt
     7801 gaatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgccg
     7861 tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatt cccttctcac
     7921 gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca
     7981 gtacatcgcc ttcgcgacct accgcttcaa cgtaacggga gaaaatatca ttgaaaccga
     8041 aatcgttacc gacgcatgaa atatgactaa cgtcagaaat agcaatcgcc gggtagcccg
     8101 gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatggcg tcgcaatggg
     8161 agccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctatct gctaacagga
     8221 ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta gccagcgttt
     8281 ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca
     8341 taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag
     8401 gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt
     8461 tgttgtaatt atattgtaaa cacattgcta aatgttttta catccactac aaccatatca
     8521 tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt
     8581 ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc
     8641 agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg gtcggcgtgg
     8701 gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg
     8761 atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact
     8821 gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt ccagggcaag
     8881 ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgcaga agagcaagcg
     8941 tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca gattactcat
     9001 gagctggcgg atctgccgcc ggagttgtgg gcgtacctta aagagcatcg cttcttcgcg
     9061 atgattatca aaaaagagta tggcggactg gagttctcag cttatgccca gtctcgcgtg
     9121 ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccgaactca
     9181 ttaggcccgg gcgagctgct gcaacattac ggcactgacg agcagaaaga tcactatctg
     9241 ccgcgtctgg cgcgtggtca ggaaatccct tgctttgcac taaccagccc ggaagcgggt
     9301 tccgatgcgg gcgcgattcc ggataccggg attgtctgca tgggtgaatg gcagggccag
     9361 caggtgctgg gcatgcgact gacctggaac aaacgctaca ttacgctggc accgattgcg
     9421 accgtgcttg ggctggcgtt taaactctcc gacccggaaa aattactcgg cggtgcagaa
     9481 gatttaggta ttacctgtgc gctgatccca accaccacgc cgggcgtgga aattggtcgt
     9541 cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc
     9601 gtgccgatcg attacatcat cggcgggccg aaaatggccg ggcaaggctg gcggatgctg
     9661 gtggagtgcc tctcggtagg ccgcggcatc acactgcctt ccaactcaac cggcggcgtg
     9721 aaatcggtag cgctggcaac cggcgcgtat gctcacattc gccgtcagtt caaaatctct
     9781 atcggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg
     9841 atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg
     9901 ctgtcggcta tcgttaagta tcactgtact caccgcgggc agcagtcgat tattgatgcg
     9961 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct
    10021 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
    10081 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
    10141 gcgaagaaca atgacgttaa cgcgttcgat aaactgctgt tcaaacatat cggtcacgtc
    10201 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
    10261 ccaaccggcg atgccactaa acgctactat cagcatctta accgccttag cgccaacctc
    10321 gccctgcttt ctgatgtctc gatggcggtg ctgggtggca gcctgaaacg tcgtgagcgt
    10381 atctcggcac gtctggggga tattttaagc cagctttacc tcgcctctgc cgttctgaag
    10441 cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacag
    10501 gatgcgctgt atcaggcaga acaggcgatg gacgatttac tgcaaaactt cccgaaccgc
    10561 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctagcaccg
    10621 tctgacaagc tggatcataa ggtggcgaag attttacaag tgccgaacgc cacccgttca
    10681 cgtattggtc gcggtcagta cctgacgccg agcgagcata acccggttgg tttactggaa
    10741 gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg
    10801 ggtaaaaatc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
    10861 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
    10921 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
    10981 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
    11041 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
    11101 cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca accaggtgag
    11161 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
    11221 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
    11281 cacctgatac gtctcatctc ccaatgtcac ggtttgtgca ggggtataag taccgccttt
    11341 catcacccag gcaggcagct tatgcccttg caccatctga ttaaatgcag ctttggtggt
    11401 ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat
    11461 gaccagtgcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc
    11521 catgtaatta acctcctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
    11581 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa
    11641 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
    11701 ttgcagcaac cactggtgtg gatggatggt cctgccaacc tgcgtcattt tgatcgtcaa
    11761 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
    11821 gccatggaag cggcagcttc gtcgctagcg caagatgacg tagtgaactg gatgacagct
    11881 aaagcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt
    11941 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgacaagcgt
    12001 catcggttcc gaatggcaga tgagcatctg cattataaag cgggcaatga gcgagtgatt
    12061 gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttccggtg
    12121 tggtcgcgca atctcaacga ttacgacctc gccctgtacg tcgccaactg gcctgctccg
    12181 cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg
    12241 gcgggatgta atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
    12301 gtgattaatc cacagggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
    12361 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcgg
    12421 gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttaaggacgg ggagagtcgg
    12481 ggagacccgc acgcagaatc aggacacaca ggcgctagca aaagagatca accaacggct
    12541 ggatgggctg ttaagccgct ggctgttgct gaaaaatacc ggacaggata tggcaacgga
    12601 caacagcacc gtgccagtcc ttccgactca ttgagtttaa ggagaataac aatggcaaat
    12661 cccgtctatc tgacactgaa cggagaactt caggggctga tttcatccgg atgctcgtca
    12721 atgccgtcca tcggtaacaa agcccagatt gcacatcagg atcagatcat ggtgacgtca
    12781 ctgtcccatg gtctgagccg ggcgcagaat gtcaaccacc aggagctgac catcacgaaa
    12841 ccggtggata aatcttcccc cctgctgggc aaggccattt cggagaatga atgtctgacc
    12901 tgtgattttg tattctatcg caccaaccgc tttggtgtta atgaaccgta ctacaaggtg
    12961 aaactgacta acgcccggat ctcaaacatt ggccttaccg ttccccatac cattaacgac
    13021 agcccaggac agccggaaga gtctgtgtca tttacctatg agagtatcaa ctgggagcac
    13081 agcgtcgccg gaaccagtgc gtacagcctt tggagtgaac ggattttcta agttacctgg
    13141 g
//
LOCUS       DAZUGK010000124        13140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZUGK010000124 DAZUGK010000000
VERSION     DAZUGK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.125"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..332
                     /locus_tag="ACHY5W_003140"
     CDS             <1..332
                     /locus_tag="ACHY5W_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4448001.1"
                     /translation="NYVGFSGRARRKEYWMFTLINAIVGAIINVIQLILGLELPYLSM
                     LYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEGTSGSNRYGN
                     DPKFGSN"
     gene            complement(383..1279)
                     /gene="yhaJ"
                     /locus_tag="ACHY5W_003141"
     CDS             complement(383..1279)
                     /gene="yhaJ"
                     /locus_tag="ACHY5W_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFS4448002.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1384..2085
                     /locus_tag="ACHY5W_003142"
     CDS             1384..2085
                     /locus_tag="ACHY5W_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFS4448003.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2108..2272
                     /gene="yhaL"
                     /locus_tag="ACHY5W_003143"
     CDS             2108..2272
                     /gene="yhaL"
                     /locus_tag="ACHY5W_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFS4448004.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2406..3716)
                     /gene="cyuA"
                     /locus_tag="ACHY5W_003144"
     CDS             complement(2406..3716)
                     /gene="cyuA"
                     /locus_tag="ACHY5W_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFS4448005.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3744..5075)
                     /locus_tag="ACHY5W_003145"
     CDS             complement(3744..5075)
                     /locus_tag="ACHY5W_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4448006.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLFSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5351..6715)
                     /gene="tdcG"
                     /locus_tag="ACHY5W_003146"
     CDS             complement(5351..6715)
                     /gene="tdcG"
                     /locus_tag="ACHY5W_003146"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS4448007.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6787..7176)
                     /locus_tag="ACHY5W_003147"
     CDS             complement(6787..7176)
                     /locus_tag="ACHY5W_003147"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFS4448008.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7190..9484)
                     /gene="tdcE"
                     /locus_tag="ACHY5W_003148"
     CDS             complement(7190..9484)
                     /gene="tdcE"
                     /locus_tag="ACHY5W_003148"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFS4448009.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9518..10726)
                     /gene="tdcD"
                     /locus_tag="ACHY5W_003149"
     CDS             complement(9518..10726)
                     /gene="tdcD"
                     /locus_tag="ACHY5W_003149"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFS4448010.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAISWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10752..12083)
                     /gene="tdcC"
                     /locus_tag="ACHY5W_003150"
     CDS             complement(10752..12083)
                     /gene="tdcC"
                     /locus_tag="ACHY5W_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFS4448011.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12105..13094)
                     /gene="tdcB"
                     /locus_tag="ACHY5W_003151"
     CDS             complement(12105..13094)
                     /gene="tdcB"
                     /locus_tag="ACHY5W_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFS4448012.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3330 a         3584 c         3135 g         3091 t
ORIGIN      
        1 aaaattatgt tggtttctct ggtcgcgcac gtcgtaaaga gtactggatg tttactctga
       61 ttaacgccat cgtcggcgct attatcaatg tcattcaatt gattttaggt ctggagcttc
      121 catatctgtc tatgctttat ttgctggcaa ccttccttcc cgtccttgcg cttgctatcc
      181 gtcgcttaca cgataccgac cgttcaggtg cctgggcctt gctctttttt gttccgttta
      241 ttggttggct cgtgcttctg gtttttttct gcacagaagg tacttctggc agcaatcgtt
      301 acggaaacga tccgaagttt ggttcaaatt aatcttagaa ttggggcgat atttcgcccc
      361 tttttattaa caataataaa tattattttc cgttaaaaag tttgggaatt tcccgcagac
      421 accaggattt cgcttccccc atactgtcac gacgccaggc cataataata tcgatctcgc
      481 tggtcgattc cgggctgacg acacgcaacc gcccttccgc aatatctttt tcgaccatcg
      541 gatacggcat cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg
      601 tgctcaccgt taagcgcggc tgtttgtcca gcagctgtac agtcaacacc gggcgctcac
      661 gagcggtatc cgccaccgca attccacgat atttcacacg cgtcacttca gataacggtt
      721 ccggctcctg atgaatcggg tgatcaggcg cggcaacgta gacgttcatt aacgtataga
      781 gcttgcgcga gttgatctcc gacgaggaac gaaaatgcat atccggcgcg ataacaatat
      841 ccgcccgccc ctgctccagc cgttcccacg cccccgccag cacttctgtg atgattgcca
      901 gttgggtatt ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta
      961 ccagcgcttc ggtcacaatg gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat
     1021 cagtagtcag tttatctgcg gcttccagca aaacgcgccc tcgctccaac aacatcctcc
     1081 cgacattggt gaatttggtg cgatggcccg agcggtcaaa cagcaccaca tccagctctt
     1141 cttccagttt ttgcatggtg tagctaagtg cggaaggcac gcgtcccagc tcatccgctg
     1201 ccgccgcaaa actgccccgg cgatcgatcg catccataac ccgtagtgct tccagcgtta
     1261 atgccctttc tttggccatt tcgttctcat tcaggaaatt tgaacatacc gggcagaata
     1321 tctggctaac aatgtagcgt ccagcccctt accataaaag gaagtaaaga gaggtcaaga
     1381 attatgatta ctacccgaac tgccaggcag tgtggacaag cagactacgg atggttgcag
     1441 gcccggtata ctttttcctt tggacactac ttcgacccga aattgttagg ctatgcctcc
     1501 ctgcgtgtgc ttaaccagga agtgctggcc ccaggtgccg cctttcagcc gcgaacctat
     1561 cccaaagtcg atattttaaa tgtgattctg gatggagaag cagagtatcg cgacagcgaa
     1621 ggcaatcatg ttcaggccag cgccggtgag gcgttgctgc tctctaccca gccgggtgtc
     1681 agctacagcg aacacaatct cagcaaagac aaaccgttaa cacgaatgca gctttggctg
     1741 gacgcctgcc cgcagcgaga gaatccgctg attcaaaaac tggcgcttaa tatgggcaag
     1801 cagcaattaa tcgcctcgcc agagggtgcg atgggaagcc tgcaattacg ccagcaagtg
     1861 tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga atttccagtt gcatgggcca
     1921 cgcgcgtatt tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa
     1981 gcggcgctga cctgcggtga tggggcgttt attcgtgacg aggctaacat tacgctggtt
     2041 gccgattccc cactgcgcgc tttgctgata gatttgcctg tctagttgtt tttacaggga
     2101 gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg tggtggcgaa
     2161 agaacctgct cgcaccgcca aaaattttgg ctatgaagag atgttgagcg agctggaagc
     2221 catcgtcgcg gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt
     2281 tgccggatgc ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat taacgaatta
     2341 aacaacccgt aggccggata agatgcgcca gcatcgcatc cggcacgata cccaaaacct
     2401 ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc
     2461 gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc
     2521 ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc
     2581 gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg
     2641 ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataacg cccatccacc
     2701 agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac
     2761 aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc
     2821 gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg
     2881 gtgatcccct ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata
     2941 cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc
     3001 aaaccgcgcg cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc
     3061 aaaccttcct gcgataacgc acaatttagc ttcgcagaat cgagaataaa gcggatcgcc
     3121 gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc
     3181 gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa caccacacca
     3241 tcgtgcgtct caatatgcac aatgttggta tgcccgccga cgatagtgac acacgcccac
     3301 ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc
     3361 ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt
     3421 gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc
     3481 gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc
     3541 agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca
     3601 accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc
     3661 tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta
     3721 aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac
     3781 aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact
     3841 ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata
     3901 gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg
     3961 aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga
     4021 cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag
     4081 aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg
     4141 ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct
     4201 tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg
     4261 acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca
     4321 atggattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga
     4381 atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc
     4441 ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg
     4501 accaccagca gcttggtcag caccatgcca gtcgaaattt tgaataacaa tttctcgccg
     4561 cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac
     4621 agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt ggtgatggcg
     4681 gtggaataga cgaacatcca aatcaccagc attacgaaat agagtgcacc taacaggatg
     4741 ccccagtttt tacctaaata accgctaatg acgctcgggt aatctttaca ttctggtgat
     4801 tctgccagcg tattaataaa caatcgctga aacagataca ttgccgggta accaatcacc
     4861 gatgagaaca aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc
     4921 cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat
     4981 ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg
     5041 tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg
     5101 gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga
     5161 gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt
     5221 tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga atattcattc
     5281 tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
     5341 gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt
     5401 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
     5461 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
     5521 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
     5581 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc
     5641 ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc tccccctgac
     5701 agccgacctc cgcgccagag atggaggcgt tcattttata cagcgcgcca atagccccgg
     5761 ctgcaagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
     5821 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
     5881 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat
     5941 tcatcggatc gttagagatg ttatcgctgg aaaccagctg acgacgcagc gctacggcac
     6001 ggcgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac
     6061 cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct ttgctgcgca
     6121 gcgctagctc gttgtgcatc atcaggccag aaatagacag gccgttgtaa tcacacattt
     6181 tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca
     6241 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta
     6301 atagctcttc ctgtgccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
     6361 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg
     6421 ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga
     6481 caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc gtggcatggc
     6541 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
     6601 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg
     6661 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
     6721 caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttacgaccg tcccgctcac
     6781 gaggctttac gcactacgta ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa
     6841 acgcgcgacc tgtacacagc tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg
     6901 cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
     6961 gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
     7021 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc aaaccggaat
     7081 ttgaccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
     7141 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
     7201 gggtaaaggt acgtgaaata acatcctgct gctgttcacg ggtcagtgcg ttgaagcgca
     7261 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
     7321 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
     7381 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt
     7441 ctttgcccag cgccgcaggc acaatcgaga aggtgtacga gatcccgtct ttagcgtagg
     7501 tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcgg tcacgaccat
     7561 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtgttac
     7621 cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg
     7681 cgttgcgata ggttggcagc gctttaattt tcttcataaa acgttcaacc aggtcgcagg
     7741 caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa
     7801 agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag
     7861 acagggagtc cgttgccacc gacaggcccg cgatgccgca cgccatggtg cgatagacat
     7921 cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
     7981 tgatattcag cgcgctgatg tactgcaccg ccagccagtc catgaagtga tcgaggctgt
     8041 ccatcacttt gtcgtaatcc agcacatcgt ccatcaacgg tgctgttttc ggcccgacct
     8101 gaatcttcag cttctcgtcc accccgccgt taattgcgta gagcagcgtt ttcgccaggt
     8161 tagcgcgtgc accaaagaac tgcatttgct taccaatcac catcgggcta acgcagcagg
     8221 caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
     8281 aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt
     8341 ccgaccaaag aatggtcagg ttaggttccg gagccggccc catagtgtgc agggtgtgca
     8401 aatagcggaa ggagtttttg gtcaccaacg tacgaccgtc cagccccatc ccgccgatca
     8461 cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcagga
     8521 agcgtaccat acggatcttc atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat
     8581 tgaggacgcc agctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgcc
     8641 ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc cagataagcg aagtagagcc
     8701 actgcaccgc ttcctgtgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg
     8761 ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga
     8821 tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt
     8881 cgcgttcacg caccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa
     8941 tgcgcccacg accatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca
     9001 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
     9061 acagatattc aaattcactg tccatctcac gaccataggc gtggaatgaa cttttaatca
     9121 tattaatgcc accgaacggg tgtaacgcac gtttcaacgg cgcatccgtt tgcaggccaa
     9181 caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa
     9241 tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc gatacggatg ccttccatta
     9301 ctttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt
     9361 cataaggtgt atagttatgt tgaataaaat cgcggacatt aatttcgctt ttccagtccg
     9421 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
     9481 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt
     9541 taactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaataa
     9601 cagcgcaaat gacacgcgca ttttcactgg aaacaattcg ctcaccacag gagttagagc
     9661 gattattcat ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac
     9721 gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca tccagccgac
     9781 gtaatgaagc tgcgtgtccg gcaatatgac gggcgattcg gtgaacaaag gttttaattg
     9841 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
     9901 aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca
     9961 ggctctggtt ggtttggctg gcgacccagg atatcgcacc aaagtcgaca tcgccactgc
    10021 gggtacccat catcaagcct tccagcggcg tcattcccat tgaggtgtca acactctgac
    10081 cgttgcgaac tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
    10141 catcttccgc cagattcagc agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc
    10201 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
    10261 aataagcttc cggagccatc gtctggtgga aactggtatc aaatacagcc acctgagtta
    10321 cgcccggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
    10381 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
    10441 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccacg ctgtcattta
    10501 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
    10561 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
    10621 cggcaatacc tgacattaat acttcacagt cgctggcatc gagtacggaa aacttaatcg
    10681 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
    10741 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
    10801 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
    10861 gcatacatcg gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc
    10921 aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc
    10981 atgctgagag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
    11041 ttcagaatca gaccattcaa gccttccagc gtccccagat agtgaccgaa gaaagatttg
    11101 aagatagcca cgagtgcgat gatggaagcc gcatattcca gtgtaattgc gaacgttgtt
    11161 ttcgtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
    11221 gctttggctt ccgccatgtt ggccggagac agcgtaaaca ggcagctaaa ggcaaagaac
    11281 atcaccactg caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg
    11341 gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt tagaaaccac gaaggaagag
    11401 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc
    11461 aggataccgt catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca
    11521 gagttccagt aagggatcag cgacaaagaa atcagcacca ggctggcgat aaacggccat
    11581 accaggtagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
    11641 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
    11701 tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa
    11761 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
    11821 accgtttccg taatgttgcc ggaaggatta gagccagaaa gacacagacg cgccagcgcc
    11881 cggtggcaat aaaatgcgat ggggtatgcc aataccaaca tcagaagaat cgggatcagt
    11941 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
    12001 aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat
    12061 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
    12121 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
    12181 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
    12241 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
    12301 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
    12361 ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt
    12421 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttctgactg tacgccaata
    12481 acacgaatgg tcgggttaat agatttaatt gccaccgcaa taccagcaat taaaccgcca
    12541 ccaccaattg gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca
    12601 atcgttccct ggccagcaat cactttcgga tcatcataag gtgggataaa aatacggcct
    12661 tccatttcga caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga
    12721 acgacttctg cggagtagtc gcacgttgcc gctactttgg attttggcgc accttttggc
    12781 atcaccactt taccgtcgat acccagcatc gcgcaggaga gggaaacccc ttgcgcatgg
    12841 ttgcccgcag aacaggccac cacgccttta cgtttttccg catcggtcag tgaacttaat
    12901 ttattaaatg cgccacgaat tttaaatgaa cccgtacgct gcatattttc aaacttcagg
    12961 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa
    13021 attcgcccag ccagtcgttg tttcgcttca ataatgtcat caatagcaac cggcagatcg
    13081 tatgtaatat gcattataaa acctcttcgc ctgaattaaa tatgtaggta accgtaaccg
//
LOCUS       DAZUGK010000125        13122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZUGK010000125 DAZUGK010000000
VERSION     DAZUGK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.126"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..145)
                     /gene="rprA"
                     /locus_tag="ACHY5W_003152"
     ncRNA           complement(38..145)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHY5W_003152"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(331..1443)
                     /gene="ydiK"
                     /locus_tag="ACHY5W_003153"
     CDS             complement(331..1443)
                     /gene="ydiK"
                     /locus_tag="ACHY5W_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFS4448013.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1832..4888
                     /locus_tag="ACHY5W_003154"
     CDS             1832..4888
                     /locus_tag="ACHY5W_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFS4448014.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNCIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRVVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKIFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTSLPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4885..5295
                     /gene="menI"
                     /locus_tag="ACHY5W_003155"
     CDS             4885..5295
                     /gene="menI"
                     /locus_tag="ACHY5W_003155"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFS4448015.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5395..5583
                     /locus_tag="ACHY5W_003156"
     CDS             5395..5583
                     /locus_tag="ACHY5W_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFS4448016.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6131..6499
                     /gene="sufA"
                     /locus_tag="ACHY5W_003157"
     CDS             6131..6499
                     /gene="sufA"
                     /locus_tag="ACHY5W_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFS4448017.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6508..7995
                     /gene="sufB"
                     /locus_tag="ACHY5W_003158"
     CDS             6508..7995
                     /gene="sufB"
                     /locus_tag="ACHY5W_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4448018.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            8005..8751
                     /gene="sufC"
                     /locus_tag="ACHY5W_003159"
     CDS             8005..8751
                     /gene="sufC"
                     /locus_tag="ACHY5W_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4448019.1"
                     /translation="MLSIKGLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8726..9997
                     /gene="sufD"
                     /locus_tag="ACHY5W_003160"
     CDS             8726..9997
                     /gene="sufD"
                     /locus_tag="ACHY5W_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4448020.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9994..11214
                     /gene="sufS"
                     /locus_tag="ACHY5W_003161"
     CDS             9994..11214
                     /gene="sufS"
                     /locus_tag="ACHY5W_003161"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFS4448021.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVMGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVRALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMMATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11227..11643
                     /gene="sufE"
                     /locus_tag="ACHY5W_003162"
     CDS             11227..11643
                     /gene="sufE"
                     /locus_tag="ACHY5W_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFS4448022.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11792..12796
                     /gene="ldtE"
                     /locus_tag="ACHY5W_003163"
     CDS             11792..12796
                     /gene="ldtE"
                     /locus_tag="ACHY5W_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFS4448023.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12859..13095)
                     /gene="lpp"
                     /locus_tag="ACHY5W_003164"
     CDS             complement(12859..13095)
                     /gene="lpp"
                     /locus_tag="ACHY5W_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFS4448024.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3287 a         3235 c         3549 g         3051 t
ORIGIN      
        1 gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga
       61 gatgggcaaa gactacacac agcaattcgt tgtttcactc aggggatttc catgcttata
      121 aatcaatatg ttgatttata accgtgagct aatagtaggc atacggactt tttgcgtcga
      181 tcagattcgt ctcattagtt taaaaactgt aaagaatttg cgacaggtac taccagctga
      241 atggcggtac gcaggaaaat atggggaaga gcaagcaaat atcaattagc ggtggttaac
      301 aatcaggcgg cagagccgcc tgatgagaaa ttacttattc ggtttctcga tttcgccaag
      361 ttcttcgaga atttcttccg gttggtccgt cggcggcggg acttcttcca cccacgcggc
      421 aaacagacgc caggaaacgg ctaacagaac cggaccaata aacagaccga tcatcccgaa
      481 agcaatcaaa ccaccaataa cgccagagag gatcaggatc agcggtaaat cggcacccat
      541 gcgaattaac attgggcgga tgacgttatc cagcgtgcca accacaccgc tccacactaa
      601 caataccgtt ccccaggtgg tatcgccggt ccagtagagc cagataatcg ccggaatcag
      661 taccggcaac gggccaagct ggacaaggca ggagaggatc attaacaccg ttagcaacgt
      721 tgcataaggt acgccggata cggcgaggcc gataccgcca agcactgcct gtactaacgc
      781 cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt gccgccagca gcacggcggc
      841 atctccgcga acgcctgcca gacgggttgc aaaatggcga atgccttgtg ccacctgttc
      901 accgcgccag tacagcaggg cactgaagag aagcatcagc gcacaatgca ccataaagcg
      961 cccgatatgc gccgcttgcc caacgaacca ggtggtggtg gtgccaatat aagggcggac
     1021 tttcgccatg atcgccgtcc cccccatatc cagcaagttg tgccagcctg catatagctt
     1081 cgcgccaatc accggaatgg tattaagcca cgccagatcg ggtaacgtca tgtcaccgct
     1141 ggaaatggct ttaattagcg ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat
     1201 agggatgata aacaccatca ctaataacag tgtcatcacc agaacggcga gagagcggcg
     1261 gccaaacatg atcttttgca aacgtaacaa taccggccag gtggcgataa ccaccgtacc
     1321 ggcccatgca aagccgagaa taaagggttg aacaatccac agacatgcca caatcatgat
     1381 ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac gaacatttac
     1441 cataactact tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa
     1501 ctcacctgca taatgatgag caatttcagc ggtttttaac aggccgattc tgcccttaat
     1561 tctgatgggc gtgttagaaa aaaatgtggt acaacaactc aggcaacacg caaacgattt
     1621 actcgtcgta tttcaagttg catatgctgc ggctacgttc gttcacccca gttacttact
     1681 gttgtaagct cctggagttc actcgcttgc cgccttcctg caactcgaaa tccatagagt
     1741 aaataaacat cacctttctg tgcgctattg cagccgcctg aaagaacagt gtatcgacct
     1801 acatcacaga ccgcaggaaa gggtcaatat aatgattcca cagatttccc aggcacccgg
     1861 cgtcgttcaa ctggtgctta attttttgca agagctggag caacaaggtt ttaccggcga
     1921 tacggcgaca agttatgccg atcgtctgac aatgtcgacc gacaacagta tttaccaact
     1981 tctccccgat gcggtggtat ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct
     2041 tgccgcgcag gaacgctatt catcgctgat ctttaccccc cgcggcggcg gcaccggcac
     2101 taacggtcag gcgctcaacc aggggattat tgttgatatg tcccgccata tgaactgcat
     2161 catcgaaatt aaccctgaag agggctgggt gcgcgttgag gccggggtga taaaagatca
     2221 actcaatcag tacctgaaac cgttcggcta cttttttgcg ccggaacttt cgaccagcaa
     2281 ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc ggtcagggat cgctggtcta
     2341 tggcaaaacg tcagatcacg tacttggcgt acgcgtggtg ttgttggggg gcgatattct
     2401 cgatacgcaa cctttacccg tcgagctggc ggaaacgctg ggtaaatcca ataccacaat
     2461 cgggcgaatt tataacacgg tttatcaacg ttgccgtcag caacgccagt taatcatcga
     2521 caacttcccc aaacttaacc gctttcttac cggttacgat ctgcgtcatg tctttaacga
     2581 tgagatgacc gagttcgacc tgacgcgcat tctgacgggt tcagaaggga cgctggcctt
     2641 tattaccgaa gcgcggctgg atattacgcg cttgcctaaa gtgcgccgtc tggtgaatgt
     2701 caaatatgac tcttttgact ctgcgctgcg taacgcgccg tttatggttg aggcgcgggc
     2761 gctttcggta gagacggtgg actcaaaagt gctgaatctg gcgcgggaag atattgtctg
     2821 gcattccgtc agcgagttga ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat
     2881 tgtggaattt gctggtgatg atgaggcgct gattgatgag cgggtaaatg cactctgtgc
     2941 gcggttggat gagctgatcg ccagtcatca agcaggtgtc atcggctggc aggtgtgccg
     3001 tgagctggcg ggggttgaac gtatctatgc aatgcgcaaa aaagccgttg gtctgcttgg
     3061 caatgccaaa ggtgccgcta agccaattcc gtttgctgag gatacctgcg taccgccgga
     3121 acacctggcg gattatattg ctgaatttcg cgcgctgctc gacagccacg gcttaagcta
     3181 cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc cgtccagcgc tggatatgtg
     3241 cgatcctcaa caagagattt tgatgaagca aatctctgat gacgtggtgg cgctgactgc
     3301 gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg aatacagccc
     3361 ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg catttgatcc
     3421 gcataaccga ctcaacccag ggaagatttg cccgccagaa ggtctcgatg cgccgatgat
     3481 gaaagtggac gcggtgaagc gcggtacatt cgatcggcag atccccattg cggtacgcca
     3541 gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta tgcttcaact ttgatgcccg
     3601 tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg attcattcac cgaaagggcg
     3661 cgcaacgctg gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg atccactcaa
     3721 actggaacaa gaactgcctg aatccggcgt cagtttgcgg acgttaattg cccgcacgcg
     3781 caatagctgg catgcgaata aaggcgaata tgacttctca cacgaagtca aagaggcgat
     3841 gtcgggctgt ctggcctgta aagcttgttc gacccagtgc cccatcaaaa ttgatgtgcc
     3901 ggagtttcgc tctcgtttcc tgcagctcta tcacacccgt tatttacgtc cgctgcgcga
     3961 ccacctcgtc gctacggtcg agagctacgc accgctgatg gcacgcgcgc cgaaaatctt
     4021 taacttcttc attaaccagc cgctgatgcg caaactctcg gaaaaacata tcggcatggt
     4081 tgatttgccg ctgctgtcgg tcccctcgct acaacaacaa atggtggggc atcgctcggc
     4141 aaacatgacg ctggaacagc ttgaagctct caatgcagag cagaaagcgc gcacggtgtt
     4201 ggtggtgcag gaccccttta ccagctatta cgatgcgcaa gtggtggcgg attttgtccg
     4261 tctggtcgaa aaattaggtt tccagcctgt gttactgcca ttttcgccaa atggcaaagc
     4321 ccagcatatt aaaggttttc ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt
     4381 cctcaaccgt atggcgaagc tgggtatgcc aatggtaggc gtcgatccgg cgctggtact
     4441 ttgttatcgc gatgaatata aactggcact gggcgaggaa cgtggcgagt ttaacgtctt
     4501 actggcgaat gaatggctgg caagcgcact tgagtcacag ccagtggcta cagtcagcgg
     4561 tgaatcatgg tatttctttg gtcactgtac cgaagttacc tccttgccgg gggcgccagc
     4621 acaatgggcc gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg
     4681 ctgcggcatg gcagggactt acggacatga agcgaaaaac cataaaaatt cgctcgggat
     4741 ctatgagtta tcctggcatc aggctatgca gcgactgccg cgtaaccgct gtctggcgac
     4801 cggatattcc tgccgtagtc aggtcaaacg ggttgaaggc acgggggtac gccatcctgt
     4861 gcaggcttta ctggagatta ttaaatgata tggaaacgaa aaatcaccct ggaagcactg
     4921 aatgccatgg gtgaaggaaa catggtgggg ttactggata ttcgctttga acatattggt
     4981 gatgacaccc ttgaagcgac aatgccagta gactcgcgga caaagcagcc tttcgggttg
     5041 ctacatggag gtgcatccgt ggtactggcc gaaagtatcg gttccgttgc cggttattta
     5101 tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca atgctaacca cgtccgctcg
     5161 gcacgagaag ggcgggtacg cggcgtatgc aaaccgttgc atctcggttc gcgtcaccag
     5221 gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg
     5281 accgccattt tgtgacgttg tcaccagaaa agtgtgacgc atattgcttt tggtcattat
     5341 ctgcacagta aacggtggta tactgtgcag gtcttgtcta aaagcgagga tgctatgtct
     5401 actcaacttg atcccaccca gctggccatt gaatttttac gccgtgacca gagcaatctt
     5461 tcacctgccc aatatctcaa acgtctgaaa caactagaac ttgaatttgc tgacctgctt
     5521 actctctctt ctgctgaatt aaaagaagaa atctactttg cctggcgtct gggcgtgcat
     5581 tgatcctgac ttaatgccgc aagttcgcca aatcgacctg gattaacctc cgtccaggtt
     5641 cgatgacctt cttaatactg cttatctacc cgcctgatat tgttcaaaaa taatactaat
     5701 cgcagtttgt gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag
     5761 agctaagcca ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg
     5821 tgtcgcacag acttaagggt tttcttattt ctgaatgctg cactttttta cctatctctt
     5881 aaatagtcat tttcaataca acttttcttt ttcatcacag ccttaatgct ccttagataa
     5941 taaccattat ctaacaatga gatacctaat tcttagcgtg cctgttaacc cacacatcag
     6001 ggtctatgct tattaaaagc aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc
     6061 tgggttgaac tgataatcat tatcactaac atgctgttat acgctgaaag cgatgaagtg
     6121 aggtaaatcg atggacatgc attcaggaac ctttaaccca caggatttcg cctggcaagg
     6181 cttaacgctg acacccgcag cggcgataca catccgcgag ctggtggcaa agcagccagg
     6241 tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc gcgggctttg gctatgtgct
     6301 cgacagtgtt agcgagccgg acaaagacga tctgctgttt gaacacgacg gcgcgaagct
     6361 gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga
     6421 aggacttaat cagatattca aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg
     6481 cgaaagcttt ggggtatagg cggtactatg tctcgtaata ctgaagcaac tgacgatgtc
     6541 aaaacctgga ccggcggccc gctgaattat aaagaaggat tcttcaccca gttagccacc
     6601 gatgagctgg caaaggggat aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat
     6661 gagccggagt ggatgctgga gtttcgtcta aacgcctatc gcgcatggct ggagatggaa
     6721 gaaccgcact ggttgaaagc gcactacgac aagctgaatt atcaggatta cagctactac
     6781 tcggcaccat cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag
     6841 caaactggcg cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga gcagttgggc
     6901 gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca ttttcgactc agtttcggtt
     6961 gccactactt atcgcgaaaa actggcggag cagggaatta ttttctgttc ctttggtgag
     7021 gcgatccacg atcacccgga actggtgcgt aaatatctcg gcaccgtggt gccggggaat
     7081 gacaacttct ttgccgcgct taatgcggcg gtagcctctg acggtacgtt tatttatgtg
     7141 cctaaaggcg tgcgctgccc gatggaactt tccacctatt ttcgcattaa cgcggaaaaa
     7201 accgggcagt ttgagcgcac cattctggtg gccgacgaag acagctacgt cagctacatt
     7261 gaaggctgtt ccgctccggt gcgtgacagc tatcagttac acgcggcagt ggtggaagtc
     7321 atcatccata aaaacgccga ggtgaaatat tccacggtac aaaactggtt ccctggcgat
     7381 aacaacaccg gcggtattct caacttcgtc accaagcgtg ctctgtgcga aggcgaaaac
     7441 agcaaaatgt catggacgca atcagaaacc ggatccgcga ttacgtggaa atatcccagc
     7501 tgcattctgc gcggcgataa ctccattggt gagttttact cagtggcgtt gaccagcggt
     7561 catcagcaag cggataccgg caccaagatg atccacatcg gtaaaaacac caaatcgacc
     7621 attatctcga aagggatctc tgccggacat agtcagaaca gttatcgcgg cttagtgaaa
     7681 atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt gcgactcaat gctgattggc
     7741 gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc gcaataacag cgcacaactg
     7801 gagcacgagg caacaacatc acgtattggt gaagatcaac tgttttactg cctgcagcgc
     7861 gggatcagcg aagaagacgc catctcgatg attgttaacg gtttctgcaa agacgtgttc
     7921 tcggaactgc cgctggaatt tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa
     7981 cacagcgtcg gataaggaat aaacatgtta agtattaaag gtttacacgt cagcgtggaa
     8041 gataaagcta ttctgcgcgg attaagcctc gacgttcgtc ccggcgaagt tcacgccatt
     8101 atggggccaa acggttcggg caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat
     8161 tatgaagtga cgggcggcac ggttgagttc aaaggcaaag atttgcttgc gctgtcgccg
     8221 gaagatcgtg caggcgaagg catctttatg gccttccagt atccggtgga gattccaggt
     8281 gtcagtaacc agtttttcct gcaaacggca cttaatgcgg tgcgcagcta tcgcggccag
     8341 gaaacgctcg accgctttga ttttcaggat ttgatggaag agaaaatcgc tctcctgaag
     8401 atgccggaag atttattaac ccgttcggta aacgttggtt tttccggcgg cgagaaaaag
     8461 cgcaacgata ttttgcaaat ggcggtgctg gaaccggggt tatgcattct tgatgaatcg
     8521 gactccgggc tggatattga cgcattaaaa gtggttgccg atggcgtgaa ctcgctgcgt
     8581 gacggcaagc gctcattcat cattgttacg cactaccaac gcattctcga ctacatcaag
     8641 cctgattacg tccatgtgct atatcaggga cgaattgtga aatccggcga tttcacgttg
     8701 gtcaaacaac tggaggagca gggttatggc tggcttaccg aacagcagta acgcactgca
     8761 acagtggcat catttgtttg aagctgaagg ggtgaaacgt tccccgcaag cacaacagca
     8821 tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa catgaaaact ggaaatatac
     8881 gccgctggaa gggctgacca atagtcagtt tgtcagcatt gcgggagaga tatccccaca
     8941 gcagcgagat gccttagcgt taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg
     9001 ttacgtgcct gcgctgagcg atgcgactga aggcagcggg tatgaagtga gcattaacga
     9061 cgaccgtcag gggttacccg atgctattca ggcggaagtg tttctgcatt tgacggaaag
     9121 cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt caacggccgg caaagccatt
     9181 gctgttaatg catatcaccc agggcgtggc aggtgaagag gtgaacactg cccattaccg
     9241 acatcatctg gatctggcgg aaggtgccga agcaacggtg atcgaacatt ttgtcagcct
     9301 taatgatgct cgtcatttta ccggggcacg gttcactatc aacgtcgcag cgaatgccca
     9361 cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt caccactttg cccataacga
     9421 tttgttgctg gctgacgatg ccaccgcatt tagccacagt ttcctgctgg gtggcgcagt
     9481 gttacgacac aacaccagta cgcaactcaa tggcgaaaac agcatgttgc ggatcaatag
     9541 cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt acctggctgg aacacaataa
     9601 aggtttttgt aacagccgac agttgcacaa aactatcgtc agcgacaaag gccgcgcggt
     9661 atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa acggatggtc agatgaccaa
     9721 caataatctg ctgatgggca aactggcgga agtggatacg aaaccgcagc tggaaatcta
     9781 tgcagatgat gtgaaatgca gccacggcgc gacggtgggg cgtattgatg atgaacagat
     9841 gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc cagcagatga tcatttacgc
     9901 ctttgctgcc gaactgacgg aagcactgcg tgatgagggg cttaaacagc aggtgctggc
     9961 ccgaatcggt caacggctgc caggaggtgc aagatgactt tttccgtcga caaagtgcgg
    10021 gccgactttc cggtgctttc gcgtgaggta aacggtttgc cgctggctta tctcgacagc
    10081 gccgccagtg cgcagaaacc gagccaggtg attgacgccg aggccgagtt ttatcgtcat
    10141 ggctacgcgg cggtgcatcg tggtattcat accttaagcg cccaggcgac cgagaaaatg
    10201 gagaacgtgc gcaagcgggc atcgctgttt attaatgccc gttcggcgga agagctggtg
    10261 ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca atagctgggg caacagcaac
    10321 gtgcgggcgg gcgataacat catcataagt cagatggagc accacgctaa cattgttccc
    10381 tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg tgatcccgct caaccccgac
    10441 ggtacgttgc aactggagac gctgcctaat ctgttcgatg agaaaactcg cctgctggca
    10501 attactcatg tctccaacgt gatgggaaca gaaaatccgc tggcggaaat gatcacgctt
    10561 gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc aggcggtgat gcatcatccg
    10621 gtggatgttc gggcgctgga ttgcgacttt tacgtgttct ccgggcataa actgtatggc
    10681 cccaccggaa ttggcattct ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg
    10741 gaagggggcg gttctatgat ggccaccgtc agcctgagtg aaggcactac ctggaccaaa
    10801 gcaccatggc ggtttgaagc cggtacaccc aataccgggg gcatcattgg tcttggcgcg
    10861 gcgctggagt atgtttcggc gctggggctt aataacatag ccgagtatga acagaatctg
    10921 atgcattacg cgctatcaca gttggaatct gtaccggatc tcactctcta tggtccgcag
    10981 aacaggcttg gcgttattgc ttttaatctc ggtaaacacc acgcctatga tgttggcagt
    11041 tttctcgata attacggcat tgctgtgcgt accggacatc actgcgctat gccattaatg
    11101 gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa
    11161 gaagtggatc gtctggtgac cggcctgcaa cgtattcacc gtctgctggg ataacaggga
    11221 ggcactatgg ctttattgcc ggataaagaa aaattgctgc gtaatttttt acgctgcgcc
    11281 aactgggaag agaaatatct ctacattatt gagctgggcc agcgtctgcc agaattacgc
    11341 gacgaagaca gaagcccaca aaatagcatt cagggttgcc agagtcaggt gtggattgtc
    11401 atgcgccaga atgcgcaggg aattattgaa ttgcaaggcg acagcgatgc ggcgattgta
    11461 aaagggctta ttgcggtcgt atttattctc tacgatcaga tgacgccgca ggatattgtc
    11521 aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca cccaacatct caccccatct
    11581 cgttcacaag gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc
    11641 taaactaaag ggacagcttt catccggttg gctcacattg ccggatggtc aatgtttaca
    11701 ctgacctgca caggagtgtt tcggtttcag gtgaacataa gcgccatccg acgtttcagc
    11761 gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc ttacactcac
    11821 gcttatcggc gcttttagcg ccatccaggc tgcctgggcg gttgattatc cgctaccacc
    11881 aaccggaagc cgactggttg ggcaaaatca aacgtatacg gtgcaggaag gggataaaaa
    11941 ccttcaggcc atcgcccgac gttttgatac tgcggcaatg ttgatccttg aagccaataa
    12001 cactatcgcc ccggtgccaa aacccggtac gacgataact attccttcgc aactgttatt
    12061 acctgatgcg ccgcgtcagg ggattatcgt taaccttgca gagctgcgcc tttattatta
    12121 tccgccggga gaaaatatcg tgcaggttta tccgataggt attggcttgc aggggctgga
    12181 aacgccggtg atggaaacgc gtgtggggca gaaaatccct aacccaacct ggacgcctac
    12241 ggcaggcatt cgtcaacgtt cgctggagcg tggcattaaa ttaccgccag tcgttcctgc
    12301 cggaccaaat aacccgctag gacgttacgc actgcgcctc gcgcatggta atggcgaata
    12361 cctcattcat ggcaccagtg cgccggacag cgtcggtttg cgcgtcagtt cagggtgtat
    12421 tcgcatgaat gcaccggata ttaaagcctt gttctccagc gtgcggacgg gaacgccggt
    12481 gaaagtgatc aacgaaccgg tgaaatattc cgtggagcct aacgggatgc gttatgttga
    12541 agtacatcga ccactatcgg cagaagaaca gcagaacgtt cagacaatgc catacacact
    12601 gccagcaggc tttacgcaat ttaaagacaa taaggctgta gatcagaagt tagtcgataa
    12661 agcgttgtat cgtcgggcag ggtatccggt tgcggtgagc agtggagcaa ctcccacagc
    12721 cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt
    12781 acgcgcgacg cagtaatggt aaatagcaga caaaaaaatg gcgcacaatg tgcgccattt
    12841 ttcacttcac aggtactatt acttgcggta tttagtagcc atgttgtcca gacgctggtt
    12901 agcacgagct gcgtcatctt tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt
    12961 gctcagctgg tcaactttag cgttcagagt ctgaacgtca gaagacagct gatcgatttt
    13021 agcgttgctg gagcaacctg ccagcagagt agaacccagg attaccgcgc ccagtaccag
    13081 tttagtagct ttcattatta ataccctcta gattgagtta at
//
LOCUS       DAZUGK010000126        13032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZUGK010000126 DAZUGK010000000
VERSION     DAZUGK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.127"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1455
                     /gene="pitB"
                     /locus_tag="ACHY5W_003165"
     CDS             <1..1455
                     /gene="pitB"
                     /locus_tag="ACHY5W_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFS4448025.1"
                     /translation="LLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAF
                     FNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLP
                     ASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIF
                     LLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKG
                     IGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLITMEPPL
                     PAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISD
                     TSAKLAKLPGVSKEDQNLLKKLRNDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAM
                     TIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGL
                     QRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1504..2196)
                     /gene="yghT"
                     /locus_tag="ACHY5W_003166"
     CDS             complement(1504..2196)
                     /gene="yghT"
                     /locus_tag="ACHY5W_003166"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4448026.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACMRKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2370..3083
                     /gene="yghS"
                     /locus_tag="ACHY5W_003167"
     CDS             2370..3083
                     /gene="yghS"
                     /locus_tag="ACHY5W_003167"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4448027.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3115..3873
                     /locus_tag="ACHY5W_003168"
     CDS             3115..3873
                     /locus_tag="ACHY5W_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448028.1"
                     /translation="MDALQTQTVISTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            3919..5268
                     /locus_tag="ACHY5W_003169"
     CDS             3919..5268
                     /locus_tag="ACHY5W_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4448029.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEVYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5268..6092
                     /locus_tag="ACHY5W_003170"
     CDS             5268..6092
                     /locus_tag="ACHY5W_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448030.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPSTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHALKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6104..6664
                     /locus_tag="ACHY5W_003171"
     CDS             6104..6664
                     /locus_tag="ACHY5W_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS4448031.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGSGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6695..7765
                     /gene="lptF"
                     /locus_tag="ACHY5W_003172"
     CDS             6695..7765
                     /gene="lptF"
                     /locus_tag="ACHY5W_003172"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS4448032.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQE
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLIPG
                     VMLIGLLLLIARDFSLLQKYFR"
     gene            7762..8841
                     /gene="lptG"
                     /locus_tag="ACHY5W_003173"
     CDS             7762..8841
                     /gene="lptG"
                     /locus_tag="ACHY5W_003173"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS4448033.1"
                     /translation="MNIFSRYLIRHVFLGFTAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVMVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(8876..10048)
                     /locus_tag="ACHY5W_003174"
     CDS             complement(8876..10048)
                     /locus_tag="ACHY5W_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4448034.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10048..10296)
                     /locus_tag="ACHY5W_003175"
     CDS             complement(10048..10296)
                     /locus_tag="ACHY5W_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4448035.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10328..11242)
                     /locus_tag="ACHY5W_003176"
     CDS             complement(10328..11242)
                     /locus_tag="ACHY5W_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4448036.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKYVAEQRLTAMAGKITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11239..12927)
                     /locus_tag="ACHY5W_003177"
     CDS             complement(11239..12927)
                     /locus_tag="ACHY5W_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFS4448037.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     RVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3119 a         3233 c         3411 g         3269 t
ORIGIN      
        1 ttattacttg ctctggcatt tgtgttgttc tacgaagcaa tcaatggttt tcatgacacg
       61 gcgaatgcgg tggcaaccgt tatttatact cgtgccatgc agccacagct tgccgtggtg
      121 atggcggcat tttttaactt ttttggcgtg ttattgggcg gacttagcgt tgcctatgcc
      181 attgtccata tgttgccaac cgatttgttg ctgaatatgg ggtcaaccca cggcctggcg
      241 atggtctttt ccatgctgct ggcggcgatt atctggaacc tgggaacgtg gttctttggt
      301 ttaccggcct ccagttcgca taccttgatt ggcgcgatta tcggcatcgg tttaaccaac
      361 gcgctgttaa ccggctcatc ggtgatggat gcgttaaacc tgcgtgaagt gaccaaaatt
      421 ttctcctcgc tgattgtttc ccctatcgtc ggcctggtca ttgcgggagg cctgatattc
      481 ctgctgcgac gctactggag cggaacgaaa aagcgtgacc gtattcaccg cattccggaa
      541 gatcgcaaaa agaaaaaagg caaacgtaag ccgccattct ggacgcgtat tgcgctgatt
      601 gtttccgctg cgggcgtggc gttttcgcac ggcgctaacg acgggcagaa agggatcggc
      661 ctggtgatgc tggtactggt ggggattgcc cctgctggct tcgtcgtcaa tatgaacgcg
      721 tccggctatg aaattacccg tacccgcgat gccgtcacca acttcgaaca ctacctgcaa
      781 cagcatcctg aactgccgca gaagttgatt acgatggaac ctccattgcc tgcggcatct
      841 actgatggcg cgcaagtgac ggagtttcac tgtcatccgg caaatacctt tgatgcgatt
      901 gcgcgcgtta aaacgatgct gccaggcaat atggaaagtt acgagccgtt aagcgtgagt
      961 cagcgcagcc agctgcgccg cattatgctg tgcatctctg atacctccgc gaagctggcg
     1021 aaactgccag gcgtcagtaa agaagaccag aacctgctga aaaaacttcg caacgatatg
     1081 ttaagcacca ttgagtacgc tccggtgtgg atcatcatgg cggtagcact ggcgctcggc
     1141 attggcacca tgattggctg gcgtcgtgta gcgatgacca tcggtgagaa gattggtaag
     1201 cgcggcatga cgtatgcgca aggcatggcg gcacaaatga cggcggcagt gtctatcggt
     1261 cttgccagtt atattgggat gcccgtctcc acaacacacg tcctctcgtc tgcagttgca
     1321 gggacgatgg tggtggacgg cggtgggtta cagcgtaaaa cggtaaccag catcctgatg
     1381 gcatgggtgt ttactttacc ggcggcaatt tttctttctg gtgggctgta ctggatagca
     1441 ttgcagttga tttaaccttc ctgaaaatgc ccggtcctgg cgatcgggca tttccatatt
     1501 tggctagtga taaccacgcg cggtcataaa atccgtaatc gctttttctg catcaaccaa
     1561 cacctgttcc agtggctgat tggcatcgat atcaaccagt tgtgcaccac caaaggttaa
     1621 ctgtggcgtt atggcaatct tcctcgccag cgattcccgt ttatggtcgg gtttacgcat
     1681 gcaggcaact tcaaggtcaa cattgagttt gatgaccaga tcaggcttat ggctcgccat
     1741 ccagtgaaac gctttacgtt cctggcttgc cagccatgag acaaaacgac caccttcaac
     1801 gttaggtggg aacaccgtac catcgtaagc gccaggaatt tggtcctgag gataacggtc
     1861 ggttagaaca attaacccgc gacgacgaca ggcaagcata tgacgaaagc gcagtaagcg
     1921 acgggcgaca aacgctgtaa ttaccagcgc cggaactggt cctggcaatt tttttgctgt
     1981 tttcacctga tttcgttcaa ttgttttatg taaggatttt cccatcaacg gtaattttgt
     2041 cactgcacga ccgacatttc cggcctgttt tcctaaatga actctttcgg cagcaccata
     2101 tttttcgaca acggtaatga gatgttcgca caccgttgac ttgcctgaac catcgctacc
     2161 aataacggca attaatggag gtgttattga ctgcatgaat atatccttaa atttaaatat
     2221 ccatccaaaa tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc
     2281 acaattaacc attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat
     2341 tcaatacatt atttactgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt
     2401 ccacgccagt gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca
     2461 cggcaagcct ggtaaatgaa ctggcagcaa gaatgccaac agaacgcatt tatctcgggc
     2521 aatcgtccgg gcgaattggc gaatggattt cacagctccc tgttattggc gcaccttttg
     2581 ggcgttattt gcgaagtaaa gcggcacatg tgcacgaaaa gcccttaaca ccgcctggca
     2641 atattactgc tctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa
     2701 tgttgtgtaa aagccagcaa ggctttctgc tcatcaccga ccgctacccg caggttgaag
     2761 tgccggggtt tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga
     2821 taaaaatgtt aaggcagcgc gagctgaagc tgtaccaatg gatggcgtct tatttgcccg
     2881 tattgttgat tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc
     2941 aactggcagc gttacaggaa aaaatcgccg ttacgccgca attgacattc aatggcgcaa
     3001 agatccttga actggatggt cggcaaccag ccgatgaaat tctgcaagcg tcactacgcg
     3061 caattcacgc cgcgctttct tgatcaccaa aatggatagg ggatgattta acgaatggat
     3121 gcactacaaa cacaaactgt tattagtaca accgcgccgc agcccaacta cattccgggg
     3181 ctgattgcgg tggtcgggtg tgatggcacc ggtaaatcca cactgaccac cgacctggtg
     3241 aaatcgctgc aacaacactg gcaaaccgag cggcgctatc tggggctgct ctccggcgaa
     3301 gacggcgaca aaatcaaacg attgccgttg gttggcgtct ggctggaacg gcgactggcg
     3361 gccaaatcct cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg
     3421 attatgtact gcttctcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg
     3481 caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat
     3541 tatgacggac cggggattgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg
     3601 cagcgcgaac ggcgtttata ccaacaaatg gcgcaatatc gcccggaatt aattattcgc
     3661 ctgggcattg atattgagac tgccatctcc cgcaagcctg accatgacta tgccgagctg
     3721 caggacaaaa tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt
     3781 gattccagag cgccctacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt
     3841 gccatcgttt ctgaccgccg aagtttaact taaggctggc gggatacttt ttactatcaa
     3901 cgataaggtc aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg
     3961 tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcgt gagcgctttg
     4021 ctgggtctgt tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta
     4081 ctggtgattg tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg
     4141 cagctggtgg ttcagtacgg aacaccagca ttaaccaaca ataatccgca gcaatttcgc
     4201 aatgtcgtct cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc
     4261 ggcattgcct tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg
     4321 ctggcggcgc tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt
     4381 ctgcgtgcgg tagatcgctt tgatttgatt gctgtacagc aggcgacgaa accttttctg
     4441 cgcgcagcgg ggagcgtcgc agcctggtat tttgactttg gttttgcggg ttttgttatt
     4501 gcctggtacg tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcacgaa
     4561 ttacgccgca gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac
     4621 attaaaggcg cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg
     4681 cgtaactcgt gcagcacagt gttggtgggg atcgtgttag gacccgctgc cgccgggtta
     4741 tttaaaatcg ccatgacatt cttcgacgcc gccggaacgc cagcgggttt gctgggtaaa
     4801 agtttttacc cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggt
     4861 gtgaagtccg gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc
     4921 gtcggcaagc cgctcatttc gctggtgttt ggcgttaagt atctcgaagt gtatgacctg
     4981 attcaggtga tgctgggcgc aattgtgatt tcgatgctgg gtttcccgca ggagtcattg
     5041 ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc
     5101 tacatcgtat tgctgttcat gttctgtcat ctgtttggcg tgctgggagc cgcgtttgct
     5161 tacttcggtg ggcaatgtct ggacgtggcg ctctcgttaa ttccaacctt aaaggcattt
     5221 tttcagcgcc attccttgct ttataacgca gccggagaga aatcctgatg atgagaaaat
     5281 atttcccgct tgaggcgagc gagcgtctat ttgttgcgat tgaagaagat gatgtcgttg
     5341 atgcacaggt tagtttgccg tcgactattg cactaagttg taccaccgaa atcattcatg
     5401 ataattacgc gttatgcctg caattctggc tcaatggcgt taatcgtcag gaattactgc
     5461 gtttagttcg caaacaagcc aaaggcgatg agctgactgc tgatgagcgc aaacaattta
     5521 aatacatgcg agcgcgctac aagcacctgc gttttgcgca acggctgtat ttaaagaagc
     5581 atcaggccgg atttttattc ggcaaaacga ccgtttttct gggacgtttt caggacggat
     5641 ttcgtaacgg taagaaaaat attgtgtcgt attacggcaa cctgttacga atttatttaa
     5701 gctctccggt ctggtcgttg gtgaactatt cattacgcca tagccagtta gaaagcgtaa
     5761 gcggttttat tgcctatcgg caaaagcaaa tgcatgccct gaaagagata attgccaaac
     5821 cgcgactaac cggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt
     5881 attacgacac cttaagatcg cttgatcctg aaaataaaga ggcgttacag atttcccgtt
     5941 tcctcgccgc cattaatggc ctgatggggg ataaacacga cgacatggtg gcggatgata
     6001 tggaaaaccg ccagtcgtat gatgcaccgg tggcgctgga tagcgatatt cgccagcgct
     6061 tagagctgct gatttcgcgt tttccgctat aaccggagca atgatgtcat ctcgtttgat
     6121 tattgcgctg attatcatgc tcttagcccc gggtgtgcag gcacacaatt tcgtcactgg
     6181 caaaacggtt acccctgttt atattcagga aggcggcgag ctgcttctga atagcgacga
     6241 cgaaattcac taccaaaagt ggaacagcac acaattggcg ggaaaagtcc gcattatcca
     6301 gtatatcgcc gggcgtaagt cagcgaagaa aaagaactca ctgttaatta aagcggttga
     6361 agcggcaaac tttccgcagg atcgttttca gcccaccacc attgtgaaca ctgatgatgc
     6421 catctttggt agtggctatt ttgttgttgg caaaatcgaa aaaaataaac gccgctatcc
     6481 gtgggcgcaa ttcgttattg atggcaatgg gctgggacgc gttgcctggc gcttgccgga
     6541 gcagagttca accattctgg tgctgaataa agctgggcaa attcagtggg caaaggacgg
     6601 cagcctgacg ccggaagagg tagatcatgt cattgcgctg gcgcagaaac tgatcaatga
     6661 ataaaaaact caaaggattg agggcgttag tgagatgaag ctggttgaac actacatcat
     6721 gcgtggtacg cgccgtctgg tgctgattat cgtcggtttt ctgatcttta ttttcgccag
     6781 ctactccgca cagcgttatc tgaccgaagc ggcaaatggg acattagctc ttgatgttgt
     6841 gctggatatc gttttctaca aagtgctgat tgcactggag atgttgttac ctgttggtct
     6901 gtatgtgtca gttggcgtga cgctagggca gatgtatacc gactcggaaa ttaccgctat
     6961 ctctgcggcg gggggcagtc cgggacgctt gtacaaagcc gttctttatc tggcgatacc
     7021 gctaagtatt tttgtcaccc ttctgtcgat gtatggtcgt ccgtgggctt atgcgcagat
     7081 ttatcaactg gagcagcagt cgcagtcgga gctggatgtt cgccagttgc gggcaaagaa
     7141 atttaacact aacgataacg gacgaatgat cctttcgcag acggttgatc aggataataa
     7201 tcgcctgact gacgcgctga tttatacttc tactgccaat cgaacccgca ttttccgcgc
     7261 ccgttcggtt gatgtggttg acccatcacc cgagaaaccg accgttatgt tgcacaacgg
     7321 cactgcctat cttctcgatc atcaggagcg tgacgacaac gaacagatct accgtaatct
     7381 gcaattacat ctgaatccgc tggatcaaag ccctaacgtc aaacgcaaag cgaaatcggt
     7441 cacggagctg gcgcgctccg tctttccagc cgatcatgcc gaactgcaat ggcgacaaag
     7501 ccgtggcctg accgcattgt tgatggcgct gctggccatt tcattaagtc gggtaaaacc
     7561 gcggcaggga cgattttcaa cgttattgcc actgacgttg ctgtttattg ccatttttta
     7621 tggcggcgat gtttgccgta cgctggtggc taatggcgcg atccctctta ttccaggttt
     7681 gtggttgata ccaggggtca tgttgattgg cttgctgctg ctcattgccc gcgacttctc
     7741 tttgttgcag aaatacttcc gatgaatatt ttcagtcgct atttgatacg tcacgtgttt
     7801 ctcggcttta cggctgcggc tggattactg cttccgcttt tcaccacctt caacctgatt
     7861 aacgaactag atgatgtcag ccccggcggc tatcgctgga cccaggcggt gatggtggtg
     7921 ctgatgacct taccgcgcac actggtggag ctttcgccgt ttatcgcatt attaggaggg
     7981 attgtcggcc tggggcagtt atcgaaaaac agtgagctta ccgccattcg cagcacgggg
     8041 ttttctatct tccgtattgc actggtggcg ctggttgccg ggatattgtg gactgtttcg
     8101 ttaggcgcga tagatgagtg ggtggcgtcg ccattgcagc aacaggcgct gcaaatcaaa
     8161 tcgaccgcca ccgcgttggg ggaggacgat gacattaccg gcaatatgct gtgggccagg
     8221 cgcggcaatg aatttgtgac ggtgaaatcg ctgaatgagc agggccagcc ggtgggcgtg
     8281 gagatatttc attatcgcga cgatctctcg cttgaatcct acatttatgc acgcagtgcc
     8341 accattgaag acgacaaaac gtgggtcctg catggtgtga atcataaaaa atggctgaat
     8401 ggcaaagaaa cgctggaaac attagataat cttgcctggc aatcggcctt caccagtatg
     8461 gatcttgaag agctatcgat gccggggaat actttttctg tccgtcagct taatcattac
     8521 atccattatt tgcaggaaac cgggcaaccc agcagcgaat accgccttgc actgtgggaa
     8581 aaactggggc aacccatcct gaccctggcg atgattttgc tggctgtgcc gtttaccttt
     8641 agtgccccgc gctcgccagg gatgggtagc cgtctcgctg taggtgtcat cgttggctta
     8701 ctcacctgga tcagctatca aatcatggtc aatctgggat tgctatttgc gttgagcgca
     8761 cctgttaccg cgctcggttt accgatagcg tttgtgctgg tggcgttgag cctggtgtat
     8821 tggtatgaca gacaacatta aacccgccgt ggcgcaacac cacggcgggc tcacattact
     8881 gcttcagagt agcaaatgcc tgaatgatgg catcgatttg ctcacgactg tgggcagcgt
     8941 ttacgctaca gcgcagcagc gtgatgcctg caggcgcggc aggtggcagg ataagattca
     9001 cataaacgcc cagcgaaata agctcgcgcc agatgcgtaa accttcctct ttcgaaccga
     9061 ttattaccgg cactaccgga ctaatatgtg aacttaactc ataacccagt ttttgcagac
     9121 cgtcatacag atggttggcg ttatccatca gcttttgccg cagctctggt tgtgtggcaa
     9181 tggttcttaa cgacgagcgc acggtcgcaa tgcaggacgg agaaggagag gcggtaaaaa
     9241 tatacgggcg gctgccgtag cgcagcactt ccatcgcttc cgaaccgaca gcaaaaccgc
     9301 caatcgaggc cagactttta ctgaaggtgc caacgataat atccacatca tcttcaacgc
     9361 cgacagcttc agccagtccg cgccccgttg cgcctaacac gccaaacgaa tgcgcttcat
     9421 cgacaatcaa ataaccaccg agacgacgtt tgatatcaac aatttccgcc agcggcgcaa
     9481 cgtcaccgag catgctgtag ataccctcaa cgatgataat cgcctctttc gcccgttcac
     9541 cgaggcgaac catgcgccgt tccagatctt tagcatcgtt atggcggaaa cgaataattt
     9601 ccgcgccacc gagagaacag gcgtcataaa tactggcgtg gctgtcggca tccagtaaca
     9661 ccacggcgtt atggtcggca agtgcgctga tgacgcctaa attagcggta taaccggttg
     9721 aaaaaacgat agcggtaggg cgattgaaaa actcggcaat ctctttctct aacgccaggt
     9781 gcggcgcata actgccgttt gccatacgag aaccggtggt gccggtgcct tgggccgcta
     9841 acgctgcctg accttcggcg atggcatcat gattaaaggt taaaccgaga taattattgg
     9901 tcccggccag aatcactttt ttattgccga tccggccttc ggtggcggaa tacacttcat
     9961 caatacaggt gccaaacggt gttaacccgt tgtcactaaa ttgcagtcgc tcgccagcaa
    10021 ggcgcgcata tttatcgtat agccccatta tttattctcc agccatggaa gtagagtttc
    10081 cattaattgc gcaggggttt tgacatccag cagaatatta ataggaatgg aaatatcgaa
    10141 tctgtcttcc agcatcatta agagatccat cactttgatc gactccaggc caagatcgtt
    10201 gacgagatcg ctgtcgggct taatttccac accgttttcg accaaatcct gtaaacagga
    10261 aaggatgtaa tccattacta tttcacgatt taccatagaa acgttaacac acctttaaac
    10321 aagacaatca aaatagcccg ttgcgtaatg cgtgttccag gctaatcccg ggaaaccaat
    10381 taatatcttc agaaatacgg ttattacttg ccgaccagtc ggcgtgggtt aattcacgaa
    10441 ttttcgagcg ggtcagcata ggttctttac ctgccaggcg actcaacgca gtactgatat
    10501 ccgcaaggca ggtgagtacc ggcagaggaa tgccaaccat ccgcacagaa ccacaacggg
    10561 cgtcggcggc aagctgtcgt acgtgttgcc agtcatagcc gccagcgacg ccatcgcata
    10621 attcataggt ttgcgtctgt acagtttcgg cgcttaacca ctgacccact gcttgcgcaa
    10681 aatcggtgac gtgcaggaaa gagagttgtg tatctggcgc accaagtcgt ggcagcaggc
    10741 cgcgcagcat ccaatcaaac agcggtttta actctttatc gccgggacca tacacggctg
    10801 tcgggcgaaa aacgcccagc gtaattttgc cagccattgc agtcagccgt tgttcggcga
    10861 cgtatttgga atttgcgtac caggagagtt cgggatggcg cgccgccagc gaagagataa
    10921 acagaaaacg ttggcaaaaa ccgctctctt ttgccgcctg catcaggcgc agactgccgt
    10981 caacgttaca gcgggtgaaa atctcttctt tgtgcccgcg cacttgtccg gcgcaatgaa
    11041 ccaccgcgct ggctccggca accagctcgc taagtgaatg agtatcttcc agcgaaccgc
    11101 gcacccaagt gagattgtca ttgacgtgag cgcgggcagt acgcgtcaat gcgcgaacgt
    11161 gaaagccgcg ggcgagcagg ttatcgataa tatatttacc gataaaaccg gtagcgcccg
    11221 tcaccgcgac agtttgattc atgccaggga ttcctgcaca tgaaggcttg cagcataagc
    11281 cttctgataa cgttttttcg cttccgcacg ggcaggcttg ccggaggacg ttcggggaat
    11341 gctgtggggc ggcaacagat cgatagccgc ggtcacgcca aattcgcttt ggatccgtgc
    11401 tgccagcgcg tggataagct gcccgcgacg ttcttcatcg ctgatccgac actggatctg
    11461 caaaatgatt ttttcctggg cggtaacaaa agcaatcgca tcgccagaat gaatttccgg
    11521 ttcctgttcc gctatatatt caatatcctg cggccagata ttacggccac gaataataat
    11581 cagatcttta atgcgtccgg tgacatacag ataaccgtcc agcagataac cgaggtcgcc
    11641 agtgtctaac cagcccgtcg cggcaatctc gtcttgcgaa gcctggtcgc caaagtaacc
    11701 gctcatcaga ctgggaccgg agatgcaaat atggcctacc acacgttccg cgaccggcat
    11761 acctgcttca ttgcggattt caataccatg ttccggcaac gctttgccgc agttgacgaa
    11821 agtcgatacg gcgcgtgtct ctgcacccgg cgcgacggct ttaccctgat attcgaggat
    11881 gtcgcgatcc acttcgttaa ccacaacccc ggaggcttca tcagagaagc tgacagccag
    11941 cgcattttct gccagtccgt agcacggcat gaaagtttta ttgtcaaagt taacctgacg
    12001 gaaacattca gcgaattgat ggagttgttc tgcggagatg ggttctgcac caataccagc
    12061 gacgcgccag caggaaagat ccagttcagc gagatctttt tcattcacgc ggcgctggca
    12121 caattcatag ccaaacggcg gcgcaacgga aacggtgccg cgatttttac tgatcaattt
    12181 aagccattgc agaggacgca tggcaaaatc ctgagtgcgc aaataatcta ctgaaagttg
    12241 cgtggcgacg ggggtcagga gaaagccgac ccgtcccata tcatggtaga aaggcagcca
    12301 ggagacgcag cggtcgccag ggcgtaattt aatgccgtcg tggcttatag cacgtagatt
    12361 agccatcact tcgcgatggg tgataatgac gccacgggga aaacgggtgc tgccggaggt
    12421 gtactggagg taggcgatat cgttcggaac tggacgctgg agcgcaacat cggcttccgg
    12481 taatgcctta aaccaggcgt ggcttaaaac atgtaattcg gggttgtcat gcgtcgcggc
    12541 attgaccagt ggcaaccact catcaccagt gataatggct gcgggctggc aacttgccag
    12601 taaaccctgc aatttggcgc tccaggaatc ccgctgacca acgcccattg gaatcgccaa
    12661 cgggacggcg actaagccgg catactggca ggcaaaaaaa gcctctacga acccgctact
    12721 ggtttcggca atcagtgcca cgcgatcgcc ttttttcagg ttcagcgata acaaccgctt
    12781 cgcaccagct tcggcacgcg cttttaacgt ctgatattcc agttgatctt caagttggca
    12841 acgtctgtca taaaaattca ttccagcgct actcagagcg gcgtagtcca aagcatcaac
    12901 cagcgttgga aaatcggcat agcgcatagg tagggaatgc gtaaagattt tattagacat
    12961 atacaccacg aaaaaattgg atatccaaaa taaaatgaca ataatgcggt ggattatata
    13021 ggcataaaag at
//
LOCUS       DAZUGK010000127        12875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZUGK010000127 DAZUGK010000000
VERSION     DAZUGK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.128"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..262
                     /locus_tag="ACHY5W_003178"
     CDS             <1..262
                     /locus_tag="ACHY5W_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4448038.1"
                     /translation="NCSVLTKAWMFNTRLYRVNFDEASVQGMGISILRGEENIPINSD
                     TLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQHAD"
     gene            complement(416..2065)
                     /locus_tag="ACHY5W_003179"
     CDS             complement(416..2065)
                     /locus_tag="ACHY5W_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFS4448039.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2216..3565)
                     /gene="ghxP"
                     /locus_tag="ACHY5W_003180"
                     /gene_synonym="yjcD"
     CDS             complement(2216..3565)
                     /gene="ghxP"
                     /locus_tag="ACHY5W_003180"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFS4448040.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4111..4575)
                     /gene="soxR"
                     /locus_tag="ACHY5W_003181"
     CDS             complement(4111..4575)
                     /gene="soxR"
                     /locus_tag="ACHY5W_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFS4448041.1"
                     /translation="MEKKLPCIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4661..4984
                     /gene="soxS"
                     /locus_tag="ACHY5W_003182"
     CDS             4661..4984
                     /gene="soxS"
                     /locus_tag="ACHY5W_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFS4448042.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(4987..6573)
                     /gene="pdeC"
                     /locus_tag="ACHY5W_003183"
     CDS             complement(4987..6573)
                     /gene="pdeC"
                     /locus_tag="ACHY5W_003183"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFS4448043.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDQSELVIHQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYFRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVNKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7000..7281
                     /locus_tag="ACHY5W_003184"
     CDS             7000..7281
                     /locus_tag="ACHY5W_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFS4448044.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7380..7916)
                     /gene="ssb1"
                     /locus_tag="ACHY5W_003185"
     CDS             complement(7380..7916)
                     /gene="ssb1"
                     /locus_tag="ACHY5W_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFS4448045.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8171..10993
                     /gene="uvrA"
                     /locus_tag="ACHY5W_003186"
     CDS             8171..10993
                     /gene="uvrA"
                     /locus_tag="ACHY5W_003186"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4448046.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADIDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11028..11384)
                     /locus_tag="ACHY5W_003187"
     CDS             complement(11028..11384)
                     /locus_tag="ACHY5W_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4448047.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11388..11804)
                     /locus_tag="ACHY5W_003188"
     CDS             complement(11388..11804)
                     /locus_tag="ACHY5W_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFS4448048.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(11915..12628)
                     /gene="aphA"
                     /locus_tag="ACHY5W_003189"
     CDS             complement(11915..12628)
                     /gene="aphA"
                     /locus_tag="ACHY5W_003189"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFS4448049.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3318 a         3372 c         3184 g         3001 t
ORIGIN      
        1 caactgttct gttttaacaa aagcctggat gtttaatacc cgtctctatc gggttaattt
       61 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg agaatattcc
      121 cattaatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
      181 tactggcatg tcacaaactg agaataatac tcatgaagta gctatgaaga ttactgcaga
      241 tattatgcaa cacgcagatt gagattaagt cgaagaacag ccaatgaaat gactttaaaa
      301 gagagataac ttatttgttt tagaatcatg aatgttagcc cgagctaacc tgttaaaggt
      361 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
      421 gattttcctc aatcaacaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
      481 tttcgttgct gatctcccgc gtggcgcgta ggtggtacag ttcagcacgt tcagaacgca
      541 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
      601 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
      661 gctggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
      721 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
      781 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
      841 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
      901 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
      961 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
     1021 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
     1081 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
     1141 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggtagctgc agacctaaca
     1201 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
     1261 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
     1321 caaccgcagc gaggatgccg gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
     1381 gcagcaggaa cagcaggacg atctgcgtcg cgggttcatc accgccccag cggctgagga
     1441 atcgcagcga gcgaccgtac agccagctaa ccacaaaacc ggcgagaata ccgccaatgg
     1501 ctactttcat aaattcgacc gtcgcaccgc cgacggtgaa gatcatcgtc cccatcgcca
     1561 ctgctacggc aaacttcaac gacaccagac cggaggcgtc attcatcaac gcttcgccct
     1621 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
     1681 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
     1741 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagtg
     1801 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
     1861 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
     1921 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
     1981 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
     2041 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
     2101 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
     2161 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
     2221 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
     2281 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
     2341 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
     2401 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
     2461 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
     2521 acagaatcag caggaacagc acgccaacgg tgatagccgt caaaccggtt ttaccgcccg
     2581 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agccgcaccc accaggccag
     2641 agaaaacgct gctcatggag tcagtggtca gtgccttccc gccgtcgata atttgcccat
     2701 ctttatccag caggttcgcc tggccggcga cggcacggat agttccggtg gcatcaaata
     2761 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
     2821 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
     2881 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
     2941 tcagcagaat gccaccaggg actttcagtt tttccaggcc gatgatcacc gccagaccta
     3001 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
     3061 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
     3121 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
     3181 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
     3241 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagagaga
     3301 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
     3361 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
     3421 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
     3481 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
     3541 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
     3601 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcgac
     3661 aatttgctgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
     3721 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
     3781 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
     3841 tgcgcaagat tgcagcgtta gcgagccgta taccgttctg gattgcgata aaaaatcgtc
     3901 tgagatgaca aaaatattgc ggctaaaaag tatactttta cactggcgaa gaaacgtcgg
     3961 tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
     4021 tgttgacgtc gggggaaacc ctcctgtgta ccagcgggat agagagaaag acaaagaccg
     4081 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc
     4141 gccggtacct tcttctccta agcggtcgcc cgggttacgc aacgggcaat cactgcgcga
     4201 aaggcagcca caaccaatac atccgtccag ttcgtcacgc agcgccacta aggtatgaat
     4261 gcgccgatct aattcttctc gccattgcga agaaagctgc ttccactctt tggcgcttaa
     4321 tgtatgccct tcgggcaaca cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat
     4381 acgctgagca attttgataa ttgcgacata tcgcaacaca tcacgtttat atcgccgctg
     4441 attgccgctg ttacggatac tggtaatcaa ccctttgctt tcatagaaat gcagcgccga
     4501 taccgccaca ccgctgcgtt tcgccacttc gccgggggtt agcagcgctt taatgcaggg
     4561 taatttcttt tccataaatc gctttacctc aagttaactt gaggaattat actccccaac
     4621 agatgaatta acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat
     4681 tcaggatctt atcgcatgga ttgacgagca tattgaccag ccgcttaaca ttgatgttgt
     4741 cgcaaaaaaa tcaggctatt caaagtggta cttgcaacgg atgttccgca cggtgacgca
     4801 tcagacgctt ggcgattaca ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg
     4861 caccaccgag cgtccgattt ttgatatcgc aatggacctg ggttatgtct cgcagcagac
     4921 cttctcccgc gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct
     4981 gtagttttat tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc attgcataaa
     5041 cacctgcggc ggcatcgcct tcgcaaagaa ccatccctgg caatagcgca cgccgcgttt
     5101 gcgcagccag ttaacctgct cctccgtttc gacgccttca gcgattgttt ttaaccccag
     5161 gctgtgcgcc agctcgatga tatgttccgc aatcaaatga ctggttttat gggtggtcag
     5221 cgtctcaaca aacgatttgt cgattttcaa aatatcgaca ttcaatgatt taaggttatg
     5281 caagttagag tagccaatac caaaatcatc aattgccact tcgtaaccgg cctggcggaa
     5341 agccagaata atcggcgtca ttttgttgac atcaagaaat gcatgctcag tcacttcaaa
     5401 tttaatttgc tgcggacgca ccgcgtattg ctctgttttc tgattgattc gcgctatcaa
     5461 ccgtgacgta tgaaaatcgg aggccgacag attaatagaa acatagcgat cagcatgtgt
     5521 tgcaaggtac gcgcccagat cgcggaagac attatcaata acataatcag ttacctgttc
     5581 tatcatcccc tccttttctg ccagcggaat aaactctgcc ggattcatta cttgcccctg
     5641 ctcaccaggc caacgtaaca acgcttcagc gccgatacat ttttctgttt tgatatcgat
     5701 tattggctgg taataaagac aaagttgatg tttttctagg gcgcgttgca atttgcgttt
     5761 gggagacaaa tagttttgtc gaatacgtag ccagagtaat agcagaacaa gactccccaa
     5821 aataccggcg ggtaacgcaa aaataagatg attataaaaa tgggctataa gacgttgata
     5881 tgaagtcgca acaatggctg caattgggcg ttttgtcgaa taaactgtcg catataaata
     5941 gccatttctt tgtacggtta aatccttcaa atgaatcagc ggagaaaacg ttgctgccga
     6001 ggcctcgttg cttaacgaga aaaaggtttt cgtcaccgta tcatacacac cccattgcaa
     6061 tgtcgggtca tcagacatca cttcactcca gaagagaggg ttgataaccg ccacataatt
     6121 tccccgctgc atataggtca ttttatagcc agagaaaaaa ggcgtatcgc ggaaataata
     6181 gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc cgtttacagg
     6241 cgctatcagt gatgagcata aaaaatggtt atcacgggca tagatcaatt cattaatata
     6301 aagatagcca cgaataatat ttaacattcg cttttgatgg gctggagagc aaacttgccc
     6361 ctgatagcgt tctgctgcat cgctcactaa atctgcctgg tgaatgacca gctcggattg
     6421 atccagtgcc agttgagcaa aggttcggag ctgattattc acttctgatt ttgcccaaag
     6481 gaaggcaatc catagcgaaa gaatgatggg aaagagaact aaaaagataa tgcccggcaa
     6541 cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc ctgtttatac
     6601 ccggccattg caaggtgaaa ccctggctac cgacgtcttt agataaatct tacggtataa
     6661 atagcacttt tgcctgattt gaaattgata cctttttaat cgtcacctgg atgaaaataa
     6721 ttcattgata ttcaaattaa ttttcttcag gaatgcaggg cggcgggaag atatgccctt
     6781 aatgccacag aaaaaccaca taatctgtac agcaataaaa gtcacgacct aatctggcta
     6841 acaaacaatc aattgtgata tagttcacaa aattaatgaa acaaacagag tgtttcattt
     6901 ttgtgttctg caagccccgc gatagccaga gtgtaccacg cctcccgtga acaacgccgc
     6961 gctgtccagg gcatcggctc ttttcagagg aattgtttga tggctaccct caccactggc
     7021 gtggttcttc ttcgctggca acttcttagt gccgtaatga tgtttctggc cagcacactc
     7081 aacatccgtt ttcgtcggtc tgattatgtc gggcttgcag tgatcagcag cggtctgggc
     7141 gtggtttccg cctgctggtt cgcaatgggg ttgcttggca tcacaatggc ggacatcacc
     7201 gccatctggc acaacatcga gtcggtgatg atagaagaga tgaatcagac accgccacaa
     7261 tggccaatga ttttgacctg atacgtagaa gcctccataa cggaggcttc ttttttatgg
     7321 ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt aatgacaaat
     7381 cagaacggaa tatcgtcatc aaagtccatc ggcggctcgt tagacggcgc tgccggagcg
     7441 gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg cggctgctga
     7501 ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc aggagcacca
     7561 ccaccctgac gaccacccag catctgcatg gtaccgccaa cgttcaccac gacttctgtg
     7621 gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg accttcgata
     7681 taaacctgag aacctttacg cagatattcg ctggctactt ctgccagttt gccgaacagc
     7741 acaacgcggt gccattcagt ctgctctttc atctcgccgg tcgctttatc acgccaggat
     7801 tcggaagtag ccagcgtaat gttggcaaca gcgccaccat ttggcatgta gcgtacttcc
     7861 gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct ggccatgttc
     7921 gtgtctcctg aaaaaaaatc gttctgaata agtgtaaacg cgcgattgta ccattaccaa
     7981 tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca gacattgtta
     8041 cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa tttgtgtcat
     8101 aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg
     8161 ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc tcaaaaacat
     8221 caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg gttctggcaa
     8281 atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg ttgaatccct
     8341 ttccgcctac gcgcggcagt ttctgtcact gatggaaaag ccggacgtcg atcatattga
     8401 ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc cgcgttctac
     8461 ggtggggaca atcaccgaaa tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga
     8521 accgcgctgt ccggaccacg acgtcccgct ggcggcgcaa accgtcagcc agatggtgga
     8581 taacgtgctg tcgcagccgg aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga
     8641 gcgcaaaggc gaacacacca aaacgctgga gaacctggca agccagggtt acatccgtgc
     8701 tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac tgcaaaagaa
     8761 acacactatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctta cccaacgtct
     8821 ggccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcagtag tggcggatat
     8881 agacgacccg aaagcggaag agctgctgtt ttccgccaac ttcgcctgcc caatttgcgg
     8941 ctacagtatg cgcgagctgg agccgcgact gttttcgttt aacaacccgg cgggggcctg
     9001 cccgacctgc gacggccttg gcgtacagca atatttcgat cctgaccgcg tgatccagaa
     9061 tccggaactg tcgctggcag gtggtgcgat ccgtggctgg gatcgccgca acttctatta
     9121 ttttcagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag cgccgtgggg
     9181 cagcctgagc gcgaacgtgc ataaagtggt gttgtacggt tctggcaaag aaaacattga
     9241 attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt
     9301 gctgcacaat atggagcgcc gttataaaga gacggagtcc agcgcggtac gcgaagaatt
     9361 agccaagttt atcagtaatc gtccgtgtgc cagctgcgaa gggacgcgtc tgcgtcggga
     9421 agcgcgccac gtgtatgtcg agaatacgcc gctgcccgcc atctccgaca tgagcattgg
     9481 tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga agattgcgga
     9541 aaaaattctt aaagagatcg gcgatcgttt gaaattcctc gttaacgtcg gcctgaatta
     9601 cctgacactt tcccgctcgg cagaaacgct ttctggcggt gaagcccagc gtatccgtct
     9661 ggcgagccag attggtgcgg gcctggttgg tgttatgtat gtactggacg agccgtctat
     9721 cggcctgcac cagcgcgata acgagcgcct gttgggtaca cttatccatc tgcgcgatct
     9781 cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgccg ctgatcatgt
     9841 gatcgatatc ggtcctggtg caggtgtaca tggcggtgaa gtggtcgcgg aaggtccgct
     9901 ggaagcgatt atggcggtgc cggagtcgtt gaccgggcag tacatgagcg gtaaacgcaa
     9961 gattgaagtg ccgaagaaac gcgttccggc gaatccggaa aaagtgctga agctgactgg
    10021 tgcacgcggt aataacctga aagacgtgac gctaacgctg ccagttggtc tgtttacctg
    10081 catcacaggg gtttcaggtt ccggtaaatc gacactgatt aacgacacac tgttcccgat
    10141 tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca
    10201 gggactggag catttcgata aagtgatcga tatcgaccaa agcccaattg gtcgtactcc
    10261 gcgttctaac ccggcgacct ataccggcgt gtttactcct gtgcgcgaac tgtttgcggg
    10321 cgtaccggaa tcccgtgcgc gcggctatac gccgggacgt ttcagcttta acgtccgtgg
    10381 cggacgctgc gaagcctgtc agggcgacgg cgtgatcaaa gtggagatgc acttcctgcc
    10441 ggacatttac gtaccgtgcg accagtgtaa aggtaaacgc tataaccgtg aaacgctgga
    10501 aattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg aagaggcgcg
    10561 tgagttcttt gatgcggtgc cagctctggc gcgtaagctg caaacgttga tggacgttgg
    10621 tctgacgtac attcgcctgg ggcagtccgc aaccacactt tctggtggtg aagcccagcg
    10681 cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt atattctcga
    10741 cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg tgctgcataa
    10801 actgcgcgat cagggcaata ccatcgtggt gattgagcac aatctcgacg tgatcaaaac
    10861 tgctgactgg attgtcgacc tgggtccgga aggcggcagt ggcggcggcg agatcctcgt
    10921 ctccggtaca ccagaaaccg tcgcggagtg cgaagcatca cacacggcac gcttccttaa
    10981 gccgatgctg taatcgttaa ggccgctttc tgagcggcct tttcctttca gagttgcacc
    11041 agcaatttac gtttttcttc cggcagtaaa ttcaccgcct gctgataaga cgcatccacc
    11101 agataataga tttgcgaatc tggcagcgaa ccgtcgagat acacggtgct ccagtgcgct
    11161 ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag ctccgccagc
    11221 tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac catcgcaaac
    11281 agtacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg
    11341 cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttta ctccccttgt
    11401 agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag ccaaatgcct
    11461 tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga tgttcccagc
    11521 attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg ctcatagttt
    11581 ccattatcgg gaacggtgcg gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg
    11641 cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg cagtaagccg
    11701 atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac cagatgaaac
    11761 ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg ctccttgata
    11821 aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac
    11881 agcattcggg agagtgtcac gctctcccgc tctgtcagta ttctgaattg acgatcacct
    11941 cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa
    12001 tgcggatacc acgagcgccg acatcgcgtg cagcggtaat atcgttatcg gaatcgccat
    12061 aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc cctggtttat
    12121 cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta tccgccagcg
    12181 tttttgaaac cgtttctgtt ttcgtcgggc tacgaccagt cacaaagaag attgcgtcac
    12241 cacggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg ctgaattcat
    12301 cccagccatt gttcattttt tcccagaaca caggattttt cagataatct tcgctttctg
    12361 gcgagaaggt ttttttgccg cgccagaagc ccggactgga aaaaagtacc gtgtcatcga
    12421 tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg
    12481 aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtacca gggttaagcg
    12541 gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat aagcaaacgg
    12601 cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta tgttgaaact
    12661 ttttattgtg agatttgttg caaaaaccat ctgaccataa cgccagcagc cggggaaagg
    12721 aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac ccagtaaata
    12781 cgttcctgtc tttttttgta taaaaaatgt ttattactcg atgtaaggat acattaattt
    12841 tacgtttatt atgatgaatc ctcggcaaat gagcg
//
LOCUS       DAZUGK010000128        12814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZUGK010000128 DAZUGK010000000
VERSION     DAZUGK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.129"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(169..1527)
                     /gene="plaP"
                     /locus_tag="ACHY5W_003190"
     CDS             complement(169..1527)
                     /gene="plaP"
                     /locus_tag="ACHY5W_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFS4448050.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1517..1579)
                     /gene="yoeI"
                     /locus_tag="ACHY5W_003191"
     CDS             complement(1517..1579)
                     /gene="yoeI"
                     /locus_tag="ACHY5W_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFS4448051.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1794..2723)
                     /gene="yeeY"
                     /locus_tag="ACHY5W_003192"
     CDS             complement(1794..2723)
                     /gene="yeeY"
                     /locus_tag="ACHY5W_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4448052.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2769..3593)
                     /gene="yeeZ"
                     /locus_tag="ACHY5W_003193"
     CDS             complement(2769..3593)
                     /gene="yeeZ"
                     /locus_tag="ACHY5W_003193"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4448053.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTSRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3676..3930)
                     /gene="yoeB"
                     /locus_tag="ACHY5W_003194"
     CDS             complement(3676..3930)
                     /gene="yoeB"
                     /locus_tag="ACHY5W_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFS4448054.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3927..4178)
                     /gene="yefM"
                     /locus_tag="ACHY5W_003195"
     CDS             complement(3927..4178)
                     /gene="yefM"
                     /locus_tag="ACHY5W_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFS4448055.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4462..4512
                     /gene="hisL"
                     /locus_tag="ACHY5W_003196"
     CDS             4462..4512
                     /gene="hisL"
                     /locus_tag="ACHY5W_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFS4448056.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4488..4611
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4658..5557
                     /gene="hisG"
                     /locus_tag="ACHY5W_003197"
     CDS             4658..5557
                     /gene="hisG"
                     /locus_tag="ACHY5W_003197"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFS4448057.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMMG"
     gene            5563..6867
                     /gene="hisD"
                     /locus_tag="ACHY5W_003198"
     CDS             5563..6867
                     /gene="hisD"
                     /locus_tag="ACHY5W_003198"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFS4448058.1"
                     /translation="MSFYTIIDWSSCTTEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6864..7934
                     /gene="hisC"
                     /locus_tag="ACHY5W_003199"
     CDS             6864..7934
                     /gene="hisC"
                     /locus_tag="ACHY5W_003199"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFS4448059.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITIGTREESQCVIDALRAEQV"
     gene            7934..9001
                     /gene="hisB"
                     /locus_tag="ACHY5W_003200"
     CDS             7934..9001
                     /gene="hisB"
                     /locus_tag="ACHY5W_003200"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFS4448060.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWSMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9001..9591
                     /gene="hisH"
                     /locus_tag="ACHY5W_003201"
     CDS             9001..9591
                     /gene="hisH"
                     /locus_tag="ACHY5W_003201"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFS4448061.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKLTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGVAGAKLLKNFLEM"
     gene            9591..10328
                     /gene="hisA"
                     /locus_tag="ACHY5W_003202"
     CDS             9591..10328
                     /gene="hisA"
                     /locus_tag="ACHY5W_003202"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFS4448062.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10310..11086
                     /gene="hisF"
                     /locus_tag="ACHY5W_003203"
     CDS             10310..11086
                     /gene="hisF"
                     /locus_tag="ACHY5W_003203"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFS4448063.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11080..11691
                     /gene="hisIE"
                     /locus_tag="ACHY5W_003204"
     CDS             11080..11691
                     /gene="hisIE"
                     /locus_tag="ACHY5W_003204"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFS4448064.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKITFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11788..12765)
                     /gene="wzzB"
                     /locus_tag="ACHY5W_003205"
     CDS             complement(11788..12765)
                     /gene="wzzB"
                     /locus_tag="ACHY5W_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFS4448065.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3214 a         3365 c         3280 g         2955 t
ORIGIN      
        1 accagatgaa tgttaatggg gaaattattc atgactggtg gaatgcagac caataaccaa
       61 attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag aataaaaaaa
      121 ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc
      181 ttcgtactgc ggaaccggat tacggaaact cttggttacg caagccaggt aaatcagacc
      241 gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc
      301 accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt
      361 cttgttacgc ttctcacgga tccagaactg cgaaataacc gacaggttaa cgaaggtgaa
      421 cgccaccagc gcaccaaagt taatcagcgc cgtagccatt accaggtcga agttgattgc
      481 cagcaaggca atcgcgccaa ccaggatgat gttcattgcc ggagtacgcc atttcgggtg
      541 tacataaccg aagaagcttt tcgggaacac accgtcacgc cccattacgt acatcagacg
      601 cgctacgcct gcatgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag
      661 cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc
      721 cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat
      781 gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc
      841 atctttggtt tcttccgaca ggttgctgat gccgtcaaag ccggtaaagg agaagcacag
      901 gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgagt
      961 gctcgccagc gtacccgcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat
     1021 caccgcgatc agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt
     1081 cagactacgc aggttaaagg cggtcatgaa ggccaccaac gccaccacaa acatccacga
     1141 tgggatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg
     1201 cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat
     1261 ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc
     1321 gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg
     1381 accatccgtg aggcctgaaa cgataccaaa agtatcaaac agcgtcatcg gctgcatata
     1441 ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga
     1501 ggtgtttgga gtaacgttat gcgacatggt cattctcctt tacggtgata accgtcgcgt
     1561 aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc gatttttagt
     1621 aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa
     1681 ataaccgacg ctgatgaaac gtcggttttt agtcattttt tgacagcggc gcattgtgcc
     1741 taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt tctttaaatg
     1801 gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt tgctaaaatt
     1861 tcatcccgcc accagccact ggcaagtcct gcggtctgtt cattccagcc aatccagacg
     1921 ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact atcgagaaaa
     1981 cgttgcgcca gataacgggg taaatagccg cagccgaggc cgctaatttg cagctccagc
     2041 ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg tgaggccgta
     2101 gaagcgccgg cctgcgcagt gtcccccacc acaatcgccc gatatcgttt aatgatacga
     2161 cggtttaacg gctcttcttc cagggctaac ggatgatgag gcgcgaccgc aaagacttgt
     2221 tccagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggttc atgcatcgcg
     2281 ccaacaataa tatccgctcg cccctgagtc agggcatccc aggaaccacc gagtacgcca
     2341 ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat cagtggcgca
     2401 agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc ccagccttca
     2461 tgcagtttga tcgcctgctt ttccagttcc cgcacggtat gcagaacttc ccgccctttc
     2521 tctaataaca tttttccggt acgggtgaat ttagcgcggt gaccgctacg atcaagcaat
     2581 tgaatattaa ggtcgctttc cagcttgtga acggtgtaac tcagagcgga cggcgtctta
     2641 tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc
     2701 aacacatcca gcagtggttt catacacgcc cccttgcagt gtatgcggcg tcctgctggc
     2761 gtgatcgttt actccagcgg cattaccagc ggatcgggat actggtattc aaaccccagt
     2821 tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag actatttctg
     2881 aactgcggag gttccagccc cagtaaacgg gccatctgcg gatagaaaac attacgcgca
     2941 gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg taacaacaga
     3001 gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc accatcaggc
     3061 gcggtttttc cggcaaagaa gcgtccggga tgacgtcccg gtccgaccag gcccgcaaga
     3121 cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat
     3181 actcgtccac tgttggttac cggattacgc gaggtggttt ctttcaccgt gccttgcgcg
     3241 tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg ggccagcgcg
     3301 ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggagcc gctacgacgt
     3361 gccggaagcg taatgaccag tgcatcggca tccatcaggg catccagatc gtcagaatcg
     3421 caaactaact caggttccat gcgaagcaga tagctatcaa tgccactcat tcgggccgct
     3481 tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac
     3541 atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt catccgttat
     3601 ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga caattcattc
     3661 tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg aatcatcagt
     3721 aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac ctgacaaatt
     3781 atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg tatctttgat
     3841 aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca gataatcatc
     3901 ccatgattcc tcagaccaga ttagtttcac tcaataatgt ccttttccgt tcctttgcct
     3961 gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga acgcagtaga
     4021 taagccgtct cttccagcga gttgtattct tcgagtgaca tcagaacaca agcctctcca
     4081 ttctgacgag taatgaggat cggggcatga tcttcaacgg ctttcatcat tgttgccgac
     4141 aaattctgac gcgcttcgct gtaactaatt gtacgcatgt caatctcctc ttttgtacag
     4201 cttattgtac aatgatagac gttgattcac tatttattaa ttagtttgta gatcaagaca
     4261 ttgtcagtga gacgaaaaat ccaggcttcg ctattttggg taccgtcagg ttagaggaca
     4321 gtcctcttag cgccctcctt tccccgctca ttcattaaac aaatccatcg ccataaaata
     4381 tataaaaaag cccttgcttt ctaacgtgaa agtggtttag gttaaaagac atcagttgaa
     4441 taaacattca tagagacttt tatgacacgc gttcaattta aacaccacca tcatcaccat
     4501 catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc
     4561 atgaacgcat gagagagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt
     4621 gataacggtt cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg
     4681 catagctatg cagaaatccg gccgtttaag tgatgactca cgcgaattgc tggcgcgctg
     4741 tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat
     4801 tgatattctg cgcgtgcgtg acgacgacat tcccggtctg gtaatggacg gcgtggtaga
     4861 ccttggaatt atcggcgaaa acgtgctgga agaagaactg cttaaccgcc gcgcccaggg
     4921 tgaagatccg cgctacttta ccctgcgtcg actggatttc ggcggctgcc gcctttcgct
     4981 ggcaacgccg gttgatgaag cctgggacgg cccgctctcc ttaaatggta aacgtatcgc
     5041 cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggcatct cttttaaatc
     5101 ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc ggactggcgg atgcgatttg
     5161 cgatctggtt tccaccggtg ccacgctgga agcaaacggc ctgcgtgaag tcgaagttat
     5221 ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa atggaagaat ccaaacagca
     5281 actgatcgac aaactgctga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata
     5341 catcatgatg cacgcaccga ccgaacgtct ggatgaagtc atcgccctgc tgccaggtgc
     5401 cgaacgccca accattctgc cgctggcggg tgaccaacaa cgcgtagcga tgcacatggt
     5461 cagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcgctgg gtgccagttc
     5521 aatcctggtc ctgccgattg agaagatgat ggggtgatcg ccatgagctt ttacacaatc
     5581 attgactgga gtagctgtac tacggagcaa caacgccagc tgttaatgcg cccggcgatc
     5641 tccgcttctg aaagcattac ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt
     5701 ggcgatgaag ccctgcgtga atacagcgcg aagtttgata aaaccacggt taccgcgctg
     5761 aaggtgtctg ctgaggaaat cgccgccgcc agcgaacgcc tgagcgacga gttaaaacag
     5821 gcgatggcgg tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa actgccgccg
     5881 gtagatgtag aaacgcagcc aggcgtacgt tgccagcagg tcacgcgccc ggtggcttca
     5941 gtgggtttgt atattcctgg cggctccgcc ccgctcttct caacggtatt aatgctggca
     6001 actccggcgc gtattgcagg ttgcaataaa gtggtgctgt gctcaccgcc gccgattgcc
     6061 gatgagatcc tttacgcggc gcagctgtgc ggcgtgcagg acgtgtttaa cgtcggcggc
     6121 gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc
     6181 ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac
     6241 ggtgcggcga tcgatatgcc cgcaggtccg tcggaagtgc tggtgattgc tgacagcggc
     6301 gctacgccgg atttcgtggc ttctgatttg ctttctcagg ctgaacacgg cccggactca
     6361 caagtgattt tactgacccc cgacgccgat atggcgcgtc gcgttgccga ggccgtcgaa
     6421 cgccaactgg cagaactgcc gcgtgccgaa accgcccgcc aggcactgag cgccagccgc
     6481 ctgatcgtga ctaaagattt agcgcagtgc gtagagattt ctaatcagta cggcccggag
     6541 cacctgatca ttcagacccg caacgcccgc gagctggtcg atggcatcac cagcgccggt
     6601 tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg actatgcctc cggtaccaac
     6661 cacgtactac cgacttacgg ttataccgcg acgtgttcca gcctcggcct ggctgatttc
     6721 cagaagcgta tgaccgtgca ggagctgtcg aaagaaggtt tctccgcgct ggcttcgacc
     6781 attgaaacac tggccgccgc cgagcgcctg actgcccaca aaaatgccgt tactttgcgt
     6841 gttaacgccc ttaaggagca agcatgaaca ccgtgactat taccgattta gcgcgtgaaa
     6901 acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct
     6961 ggctgaacgc caacgaatac cccacagccg tggagtttca gctcactcaa caaacgctca
     7021 accgctaccc ggaatgccag ccgaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg
     7081 taaaaccgga gcaggtgttg gtcagccgtg gcgcggacga aggtattgaa ctgctgattc
     7141 gcgctttttg cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt
     7201 acagcgtcag cgctgaaacc attggcgtcg agtgccgcac agtgccgacg ctggacaact
     7261 ggcaactgga cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt
     7321 gcagccccaa caaccccacc gggcaactga tcaatccgca ggattttcgc accctgctgg
     7381 agttaacccg cggtaaggcg attgtggttg ccgatgaagc ttatatcgag ttttgcccgc
     7441 aggcatcgtt agctggctgg ctggcggaat atccgcacct ggcgattttg cgtacccttt
     7501 cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca
     7561 tcaacctgct gatgaaagtg atcgccccct acccgctctc gacgccggtt gccgacattg
     7621 ccgcacaggc gttaagcccg cagggaatcg tcgccatgcg cgaacgggtg gcgcaaatta
     7681 ttgcagaacg cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt
     7741 ttgactctga aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat
     7801 ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct
     7861 gcctgcgaat taccatcgga acccgtgaag aaagccagtg cgtcattgac gccttacgtg
     7921 cggagcaagt ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag
     7981 cgaaccgccg agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt
     8041 gatcccggag ctgctgaagc tgcaaaaagc gggatataag ctggtgatga tcactaatca
     8101 ggatggtctt ggaacacaaa gtttcccgca ggcggatttc gatggcccgc acaacctgat
     8161 gatgcagatc ttcacctcgc aaggcgtaca gtttgatgaa gtgctgattt gcccacacct
     8221 gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagc gttatctcgc
     8281 tgagcaagcg atggatcgcg ccaacagtta tgtgattggc gatcgcgcga ccgacattca
     8341 actggcggaa aacatgggca ttaatggttt acgctacgac cgcgaagccc tgaactggtc
     8401 gatgattggc gaacaactca ccaaacgaga ccgttacgcc catgtagtgc gcaacaccaa
     8461 agagacgcag attgacgttc aggtatggct ggatcgcgaa ggtggcagca agattaacac
     8521 cggcgttggc ttctttgatc atatgctgga tcagatcgct acccacggcg gtttccgtat
     8581 ggaaatcaac gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg
     8641 cctggcgctg ggggaagcgt taaaaattgc cctcggcgat aaacgcggta tttgccgctt
     8701 tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc gcgctggata tctctggtcg
     8761 cccgcacctg gaatataaag ctgaatttac ctaccagcgc gtgggcgatc tcagcaccga
     8821 gatgatcgag cacttcttcc gttcgctttc atacaccatg ggcgtgacgc tacacctgaa
     8881 aaccaaaggt aaaaacgatc accaccgtgt agagagcctg ttcaaagcct ttggtcgtac
     8941 cctgcgccag gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag gagtgctgta
     9001 atgaacgtgg tgatccttga tactggctgc gccaacctga actctgtgaa gtctgccatt
     9061 gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg acgtcgtgtt gctggccgat
     9121 aaactgtttt tacccggcgt tggcaccgca caagcagcga tggaccaggt gcgtgagcgc
     9181 gagctatttg atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg
     9241 caactgctcg ggcgacgtag cgaagagagc aatggcgtcg acttgctggg catcatcgac
     9301 gaagacgtgc cgaaattgac cgactttggt ctgccactgc cacatatggg ctggaaccgc
     9361 gtttatccgc aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac
     9421 tttgttcaca gctacgcaat gccggtcaat ccgtggacca tcgcccagtg taattacggc
     9481 gaaccgttca ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag
     9541 cgttctggtg tcgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc
     9601 cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat tacggcaaac
     9661 aacgcgatta cggtaacgac ccgctgccac gcttacagga ttacgctgcg caaggtgccg
     9721 aagtgttgca tctggtggat ctgaccgggg caaaagatcc ggctaagcgt caaatcccgc
     9781 tgattaaaac cctggtcgcg ggcgttaacg tcccagtgca ggttggcggc ggtgtgcgta
     9841 gtgaagaaga cgtagcgacg ttactggaag cgggcgttgc acgcgtggtg gttggctcca
     9901 ccgcggtgaa atcaccagaa atggtgaaag gctggtttga acgtttcggt gctgatgcct
     9961 tagtgctggc gctggatgtc cgtattgacg agcaaggcaa caagcaggtg gcggtcagcg
    10021 gctggcaaga gaactcgggc gtttcactgg aacaactggt ggaaacctat ctgcccgtcg
    10081 gtctgaaaca tgtgctgtgt accgatatct cgcgcgacgg cacgctggcg ggatctaacg
    10141 tctctttata tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag tcctccggcg
    10201 gcattggcga tattaacgat gtggcggccc tgcgtggtac tggtgtgcgc ggcgtaatag
    10261 ttggtcgtgc attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa
    10321 acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg
    10381 caaccatgaa atcattggcg atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc
    10441 agatgaactg gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag
    10501 ctgggtatct cgcgtggcgg aagtaatcga cattccgttt tgtgtggcgg gtgggattaa
    10561 gtctctggaa gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc
    10621 tcctgcgctg gcggacccga cgttaattac tcgcctggcg gatcgctttg gcgtgcagtg
    10681 tattgtggtc ggcattgata cctggtacga cgccgaaacc ggtaaatatc atgtgaatca
    10741 atataccggc gatgaaagcc gcacccgcgt cactcagtgg gaaacgctcg actgggtaca
    10801 ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt
    10861 gcgtaacggt tacgacctcg agcagctgaa aaaagtacgt gaagtttgcc acgtcccgct
    10921 gattgcctcc ggtggcgcgg ggaccatgga acacttcctc gaagccttcc gcgatgccga
    10981 cgttgacggc gcgttggcag cttccgtatt ccacaaacaa ataatcaata ttggtgaatt
    11041 aaaagcgtac ctagcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt
    11101 cgcgaactgg actgggaaaa aaccgacgga ctgatgccgg tgattgtgca acacgcggta
    11161 tccggcgaag tgttaatgct gggctatatg aacccggaag ccttagacaa aaccatcgaa
    11221 agcggcaaaa tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacg
    11281 tcgggcaatt tcctcaacgt agtaaatatt gcgccggact gcgacaacga cacgctactg
    11341 gtactggcga atcctatcgg accaacctgc cacaaaggca ccagcagctg cttcggcgac
    11401 accgctcacc aatggctgtt cctgtatcaa ctggaacaac tgctcgccga acgcaaatct
    11461 gccgacccgg aaacctccta caccgccaaa ctgtatgcca gcggcaccaa acgcattgcg
    11521 cagaaagtag gtgaagaagg ggtggaaacc gcactggcag cgacggtaca tgaccgcttt
    11581 gagctgacca acgaggcatc tgacttgatg tatcacctgc tggtgttgtt gcaggatcag
    11641 gatctggatt taacaacggt gattgagaac ctgcgtaaac gtcatcagtg agttgcgggg
    11701 taagcggatg cgatgttgtc gtcgcatccg gcaaaaaaaa cgggcaaggt gtcaccaccc
    11761 tgcccatttt ctttaaaacc gaaaagatta cttcgcgttg taattacgca gagcattacg
    11821 ccccaacaca atccccgcgc caaccatgcc gcccagcagc accgccagaa tcaaggtaat
    11881 tgcctttttc gggctatcgc gacgaatagg taacgtcggt ttcatcacat aacggtaagc
    11941 atgaatatca agatcatcaa cctttaagtt ttcaatatcc aacaggtttt gacgtgtctg
    12001 atagtagttt ggtgagaaca ccaacggacg ggtcgcctca tgcttaatca tcgactccag
    12061 cgcttcgcta cccaaaagga acaacgtatc ttgcgtcaca tcttcagtct gctgaacctg
    12121 tggctttgtc acctgcgcct gattcgcata ctgcaacgct tcctgaatct gacggatacg
    12181 cagatctttc tgctcctgcg caactacttc ctgcgttctt aaagagtcct gcaagttttt
    12241 ccgtcccaga gcaatgttgt ccttgagatc cttttctaac tcttgattca ctttatcgtc
    12301 aacttgctga atgtattggg ccaacttcat ttgtgcgccc tctgcagttt gcccaacata
    12361 agaaacagtt aatggtaatt gctggttctt aacagaaggt tcgatggtaa gtttttctgg
    12421 ttcttcctga ttatccagcg tttctgctaa tgcagagaag gcagaactga agcgaccaat
    12481 taacgtctcc tgcaaatccg atactttcgg tgcagcctga ccatagataa cattcatggc
    12541 attgttatag ccagcaattt gccccacgtc gggctgagtg ataattgctg ttgacgtcca
    12601 tttctccttc gctactgcca aatatccaat agccagggca atagccacaa tgacggaaat
    12661 gatgattgtc atcttgccac gccacaactg cactagtaaa tcaatcaaat caatctgttc
    12721 cgggtcatgg ttttgcccag aaacattatt attttctact ctcatcatta ccctaactga
    12781 cggaaatttc caagtccgca tagtttagcg ataa
//
LOCUS       DAZUGK010000129        12792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZUGK010000129 DAZUGK010000000
VERSION     DAZUGK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.130"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1899)
                     /gene="espL1"
                     /locus_tag="ACHY5W_003206"
     CDS             complement(1..1899)
                     /gene="espL1"
                     /locus_tag="ACHY5W_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFS4448066.1"
                     /translation="MSENDIIIRTHYKSPHRIHIDSDTPTPSSEPINHFARQLITLLD
                     TSDLSSMLSYCFSQEFTASCRKISQNCYSTALFIINFATSPIHAENTLITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQHHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGTIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNTLTAPIAQKHLNKKMVMDILAMKTRDGEPGLFAAMENNHP
                     LCVTRFLSKIYGIAVKYNLSKINIIDLLKGATVHGTPALYIAMSKGNKDVMLSYISTL
                     DTFAKKYSFSQHQLFTLLAAKNHDNMSAVHIAIYHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            2422..4350
                     /gene="thrS"
                     /locus_tag="ACHY5W_003207"
     CDS             2422..4350
                     /gene="thrS"
                     /locus_tag="ACHY5W_003207"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4448067.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWVDKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4354..4896
                     /gene="infC"
                     /locus_tag="ACHY5W_003208"
     CDS             4354..4896
                     /gene="infC"
                     /locus_tag="ACHY5W_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4448068.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4993..5190
                     /gene="rpmI"
                     /locus_tag="ACHY5W_003209"
     CDS             4993..5190
                     /gene="rpmI"
                     /locus_tag="ACHY5W_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4448069.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5243..5599
                     /gene="rplT"
                     /locus_tag="ACHY5W_003210"
     CDS             5243..5599
                     /gene="rplT"
                     /locus_tag="ACHY5W_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4448070.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5716..5840
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5722..5766
                     /gene="pheM"
                     /locus_tag="ACHY5W_003211"
     CDS             5722..5766
                     /gene="pheM"
                     /locus_tag="ACHY5W_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFS4448071.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6049..7032
                     /gene="pheS"
                     /locus_tag="ACHY5W_003212"
     CDS             6049..7032
                     /gene="pheS"
                     /locus_tag="ACHY5W_003212"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4448072.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7047..9434
                     /gene="pheT"
                     /locus_tag="ACHY5W_003213"
     CDS             7047..9434
                     /gene="pheT"
                     /locus_tag="ACHY5W_003213"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4448073.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGKAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9439..9738
                     /gene="ihfA"
                     /locus_tag="ACHY5W_003214"
     CDS             9439..9738
                     /gene="ihfA"
                     /locus_tag="ACHY5W_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFS4448074.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9839..10819
                     /gene="btuC"
                     /locus_tag="ACHY5W_003215"
     CDS             9839..10819
                     /gene="btuC"
                     /locus_tag="ACHY5W_003215"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFS4448075.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPQAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10882..11433
                     /gene="btuE"
                     /locus_tag="ACHY5W_003216"
     CDS             10882..11433
                     /gene="btuE"
                     /locus_tag="ACHY5W_003216"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFS4448076.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVSSKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11433..12182
                     /gene="btuD"
                     /locus_tag="ACHY5W_003217"
     CDS             11433..12182
                     /gene="btuD"
                     /locus_tag="ACHY5W_003217"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFS4448077.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12260..12724
                     /gene="nlpC"
                     /locus_tag="ACHY5W_003218"
     CDS             12260..12724
                     /gene="nlpC"
                     /locus_tag="ACHY5W_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4448078.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         3280 a         2899 c         3358 g         3255 t
ORIGIN      
        1 ttataaatac gtctttaatt catcagcact aaaactcatg ctttggctga ttacatttat
       61 agcagcataa tatgtttcta cagttttata atgattatga taaatggcta tatgaacagc
      121 tgacatgttg tcatgatttt tagcggccaa cagtgtaaat aactgatgtt ggctaaaaga
      181 atattttttt gcaaaagtat ccagcgtcga tatataagat aacatgacgt ctttattacc
      241 cttgctcata gcgatgtata aagcaggggt tccatgtact gttgcgcctt ttaataaatc
      301 tatgatgtta attttgctaa ggttatattt aacagcgatt ccataaattt tagagaggaa
      361 ccgtgtgaca cacaacgggt gattattttc cattgcagca aataaccctg gttcaccatc
      421 acgtgttttc atggctaaaa tatccatcac catttttttg tttaaatgct tttgtgctat
      481 tggagcggtt aatgtattca gaaagatatc gattatctca tcgaatccat acaacatgga
      541 gatataaagt ccaggtgttt cgttagcact tttagtttgc agtaatttaa ccagattatc
      601 ttcatgaatg atgtgattat ctaccagtga ctgaatttct tgcataaata ttttcacgtt
      661 gtcagtatgt ccgttcatca tagccaaaaa taaagcaggt gtaccaacgc tattctgcgc
      721 agttattatt tcaatgattt ctttttcagg gagtatgggc aactgtctga ataatgtaat
      781 aagctgttga gtctggcctg tggccattat taacaacatt gccgatggat gtataggggc
      841 aaatggcatt cgtgttgcga tacccgtgaa aatagtccct ggaactccct caggtaatag
      901 gggcagaata gcaattgcac agttgcgaac atacctatca gcccaggtct gagcatcggg
      961 acctgaactg atgaacttat caagcgagag aaagccccgt gctgtcctgt gggtgcctct
     1021 gacggcaacg ttggtatcgt tcgggtcgta aacggacaca acataattta attgcccata
     1081 cgccgactgc tttattcgta gccttactga caaagtatga ttgtctatat ataaaatcgc
     1141 agacatatga ctgacattgt ttgccatcat tcgacagaag aaaatatcta aagcgaagcc
     1201 aaaatcctta ctcgcgataa cgcgactccc tgcaggagga tggcgaagta atgctctgaa
     1261 ctccgcgtcc cggactaagg ggacatgatg ctgtatggat tcaacggagg agtaatgttt
     1321 aatcaggttg accttgcctt ttccatttct caatgaatca agtgcgtatt gcaatgacag
     1381 atggcgacaa aaaatagcgt cagaaggaga ttgcgaaaaa aatgcactac cattgagatt
     1441 tactgttggt tgtatagttt tttctctatg gattttttta gacagtttca tacaatggtt
     1501 acgattttcg gcagttaact gttcctgttc aatataatcc agtatgcttc gcaaatggtt
     1561 agctttaata ggcgtggttt ccagtaataa ggaaatgatt tcttttttat agtgtaatgt
     1621 aatgagtgta ttttctgcat ggatgggtga agtggcaaag ttaatgataa aaagggcagt
     1681 ggaataacaa ttttgtgaaa tttttcgaca gcttgcggta aattcttgag aaaaacagta
     1741 tgacagcatc gaacttaagt cagaggtatc aagcagggtg atgagctggc gcgcaaaatg
     1801 attaataggc tctgatgaag gcgttggtgt gtcgctatcg atgtgtattc tatgaggaga
     1861 cttataatga gttctgataa tgatatcgtt ttcactcata atctattcgc cttatatatg
     1921 tctggaggtt ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa
     1981 ttgctataag aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctta
     2041 tgatcgttta aatactaccg ccaaatcatg caacgaactt tgctggattc agtttatgct
     2101 gctgtaaatc cgctcgcagt aaacctttca gacgctccgt gatgttatca gttgttcttt
     2161 aagcgttttg ctggtgtact cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga
     2221 aaggtacaat ctcccttttt ttagtgttaa catcgctcaa ccgggtggaa ggtgagaggt
     2281 taatgtcttt catttgtgta cctgattgcg aaccaattta gcatttgttg gctagatggt
     2341 ttcgcaatga actgttaata aacaaatttt tctttgtatg tgatctttcg tgtgggtcac
     2401 cactgcaaat aaggatataa aatgcctgtt ataactcttc ctgatggcag ccaacgccat
     2461 tacgaccacg ctgtaagccc catggatgtt gcgctggaca ttggtccagg tctggcgaaa
     2521 gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg cttgcgatct gattgaaaac
     2581 gacgcacaac tgtcgatcat taccgccaaa gacgaagaag gtctggagat cattcgtcac
     2641 tcctgtgcgc acctgttagg gcacgcgatt aaacaacttt ggccgcatac caaaatggca
     2701 atcggcccgg ttattgacaa cggtttttat tacgacgttg atcttgaccg cacgttaacc
     2761 caggaagatg tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa aaactacgac
     2821 gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggggag
     2881 agctacaaag tctccattct tgacgaaaac atcgcccatg atgacaagcc aggtctgtac
     2941 ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc
     3001 catcatttca aactaatgaa aacggcaggg gcttactggc gtggcgacag caacaacaaa
     3061 atgttgcaac gtatttacgg tacggcgtgg gtagacaaaa aagcgcttaa cgcttacctg
     3121 cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg
     3181 taccatatgc aggaagaagc gccgggtatg gtattctggc acaacgacgg ctggaccatc
     3241 ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag agtaccagta tcaggaagtt
     3301 aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac
     3361 aaagatgcaa tgttcaccac gtcttctgag aaccgtgaat actgcattaa gccgatgaac
     3421 tgcccgggtc acgtacaaat tttcaaccag gggctgaagt cttatcgcga tctgccgctg
     3481 cgtatggccg agtttggtag ctgccaccgt aacgagccgt caggttcgct gcatggcctg
     3541 atgcgcgtgc gtggttttac ccaggatgac gcgcatatct tctgtactga agaacaaatt
     3601 cgcgatgaag ttaacggatg tatccgttta gtctatgata tgtacagcac ttttggcttc
     3661 gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac gtattggcag cgacgaaatg
     3721 tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag aaaacaacat cccgtttgaa
     3781 tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg aatttaccct gtatgactgc
     3841 ctcgatcgtg catggcagtg cggtacagta cagctggact tctccttgcc gtctcgtctg
     3901 agcgcctcct atgtgggtga agacaacgag cgtaaggtac cggtaatgat tcaccgcgca
     3961 attcttgggt cgatggaacg tttcatcggt atcctgaccg aagaattcgc tggtttcttc
     4021 ccgacctggc ttgcgccggt tcaggttgtt atcatgaata ttaccgattc acagtctgat
     4081 tacgttaacg aattgacgca aaaactatca aatgcgggca ttcgtgttaa agcagacttg
     4141 agaaatgaga agattggctt taaaatccgc gagcacactt tgcgtcgcgt cccatatatg
     4201 ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt
     4261 aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc
     4321 agccgcagtc ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg agttcaaacg
     4381 gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg
     4441 gaaggcgagc agcttggtat tgtgagtctg agagaagctc tggagaaagc agaagaagcc
     4501 ggagtagact tagtcgagat cagccctaac gccgagccgc cggtttgtcg tataatggat
     4561 tacggcaaat tcctctatga aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa
     4621 gttatccagg ttaaggaaat taaattccgt cctggtacag atgaaggcga ctatcaggta
     4681 aaactccgca gcctgattcg ctttctcgaa gagggtgata aagccaaaat cacgctgcgt
     4741 ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa
     4801 gacgatttgc aagaactggc agtggtcgaa tccttcccaa cgaagatcga aggccgccag
     4861 atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg
     4921 gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg tttattaaca atgcgaagtg
     4981 gaagttatta aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa
     5041 accggtaaag gtggttttaa gcacaagcac gctaacctgc gtcacattct gaccaaaaaa
     5101 gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg cgatctgggc
     5161 ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg tttttaactt tttaattaga
     5221 atatagatac aggagagcac atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg
     5281 tcacaagaaa attttgaaac aagctaaagg ctactacggt gcgcgttctc gcgtataccg
     5341 cgttgccttc caggctgtta tcaaagctgg tcagtatgct taccgtgacc gtcgtcaacg
     5401 taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac gcagcagcac gtcagaacgg
     5461 tatttcttac agcaaattca tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa
     5521 gatcctggct gatatcgcag tattcgacaa agtagcgttc accgctctgg ttgaaaaagc
     5581 gaaagcagct ctggcataag ccagttgaaa gagggagcta gtctccctct tttcgtttca
     5641 acgccatcaa aacattgact tttatcgccg tagccttttc aataaaggtc ttttgaagag
     5701 taccaaaagg taacgcaagc aatgaatgct gctattttcc gcttcttttt ttactttagc
     5761 acctgaatcc aggaggctag cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc
     5821 ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa aaagccagtc tatcaggcgt
     5881 aattgtcgca gccagtctgg atacggccag tgcggagaaa ccggagcgta cttaagtacg
     5941 tgaggatttc gagcactgcc cgggaccaaa atggcaagta aaatagcctg atgggatagg
     6001 ctctaagtcc aacgaaccag tgtcaccact gacacaatga ggaaaaccat gtcacatctc
     6061 gcagaactgg ttgccagtgc aaaggcggcc attagccagg cgtcagatgt tgccgcgtta
     6121 gataatgtgc gcgtcgaata tttgggtaaa aaagggcact taacccttca gatgacgacc
     6181 ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa
     6241 gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat
     6301 gcgcgtctgg cggcggaaac gattgatgtc tctctgccag gtcgtcgcat tgaaaacggc
     6361 ggtctgcatc cggttacccg taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc
     6421 tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc ataacttcga tgctctgaac
     6481 attcctggtc accacccggc gcgcgctgac cacgacactt tctggtttga cgctacccgc
     6541 ctgctgcgta cccagacctc tggcgtacag atccgcacca tgaaagccca gcagccaccg
     6601 attcgtatca tcgcgcctgg ccgtgtttat cgtaacgact acgaccagac tcacacgccg
     6661 atgttccatc agatggaagg tctgattgtt gataccaaca tcagctttac caacctgaaa
     6721 ggcacgctgc acgacttcct gcgtaacttc tttgaggaag atttgcagat tcgcttccgt
     6781 ccttcctact tcccgtttac cgaaccttct gcagaagtgg atgtcatggg taaaaacggt
     6841 aaatggctgg aagtactggg ctgcgggatg gtgcatccga acgtgctgcg taacgttggc
     6901 atcgacccgg aagtttactc tggtttcgcc ttcgggatgg ggatggagcg tctgactatg
     6961 ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa
     7021 cagtttaaat aaggcaggaa tagattatga aattcagtga actgtggtta cgcgaatggg
     7081 tgaacccggc gattgatagc gatgcgctgg cgaatcaaat cactatggcg ggcctggaag
     7141 ttgacggtgt agaaccggtt gccggtagct tccacggcgt ggtcgttggt gaagtggttg
     7201 agtgtgcgca gcatccgaac gctgacaaac tacgtgtgac aaaagtgaat gtcggcggcg
     7261 atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg
     7321 tagcgaccat tggtgctgtt ctgccgggtg atttcaaaat taaagcggcg aagctgcgtg
     7381 gtgaaccgtc agaagggatg ctgtgctctt tctctgagct gggaatttct gacgatcata
     7441 gcggcattat tgagctgcct gcggatgcgc cgattggcac cgacatccgt gaatacctga
     7501 aacttgatga caacaccatc gaaatcagcg tgacgccaaa ccgtgccgac tgcttaggca
     7561 tcattggtgt tgcgcgtgac gttgccgtgc tgaaccagct gccgctggtt gaaccggaaa
     7621 tcgttccggt tggtgcgacc atcgacgaca cgctgccgat tacagtcgaa gcgccggaag
     7681 cctgcccgcg ttatcttggc cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc
     7741 tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg
     7801 tcaccaacta tgtgctgctc gaactgggcc agccgatgca cgctttcgat aaagatcgca
     7861 ttgaaggcgg cattgtggtg cggatggcga aagagggcga aacgctggtg ctgctcgacg
     7921 gcactgaagc gaagctgaat gccgacaccc tggttatcgc cgaccacaac aaggcgctgg
     7981 cgatgggcgg catttttggt ggcgaacact ctggcgtgaa tgacgaaacg caaaacgtcc
     8041 tgctggaatg cgcgttcttc agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc
     8101 tgcatactga tgcgtctcac cgttatgagc gtggcgttga tccggcactg cagtacaaag
     8161 cgatggaacg tgcgacccgt ttgctgatcg acatctgcgg tggtaaggct ggtccggtaa
     8221 ttgatatcac caacgaagca acgctgccga agcgtgcaac catcactcta cgtcgtagca
     8281 aactggatcg cctgatcggc catcatattg cggatgagca ggtaactgac attctgcgtc
     8341 gtctcggctg cgaagtgacc gaaggcaaag acgaatggca ggcagttgcg ccgagctggc
     8401 gtttcgatat ggagattgaa gaagatctgg ttgaagaagt cgcgcgtgtt tacggctaca
     8461 acaacatccc ggatgagccg gtacaggcaa gcctgattat gggtactcac cgtgaagctg
     8521 acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa aggctatcag gaagtgatca
     8581 cctatagctt cgttgatccg aaagtgcagc agatgatcca tcctggggtt gaagcgttac
     8641 tgctgccaag cccgatttcc gttgaaatgt cagcaatgcg tctttctctg tggaccggcc
     8701 tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa tcgtgtacgc attttcgaaa
     8761 gcggtctgcg tttcgtgcca gatactcagg caccgttggg cattcgtcag gatctgatgt
     8821 tagccggtgt gatttgcggt aaccgttacg aagagcactg gaacctggca aaagagaccg
     8881 ttgatttcta tgatttgaaa ggcgatcttg aatccgttct cgacctgacc ggtaaactga
     8941 atgaggttga gttccgtgca gaagcgaatc cggcactgca tccggggcaa tccgcagcga
     9001 tttatctgaa aggtgaacgt attggttttg ttggggttgt tcatcctgaa ctggaacgta
     9061 aactggatct taacggtcgc actctggtgt tcgaactgga gtggaacaag ctcgcagacc
     9121 gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg
     9181 tggtggtcgc agaaaacgtt cccgcagcgg atattttatc cgaatgtaag aaagttggcg
     9241 taaatcaggt agttggcgta aacttatttg acgtgtaccg cggtaagggt gttgcggagg
     9301 ggtataagag cctcgccata agcctgatcc tgcaagatac cagccgtaca ctcgaagaag
     9361 aggagattgc cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga ttccaggcat
     9421 cattgaggga ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat
     9481 aagcttgggc ttagcaagcg ggatgccaaa gaactggttg aactgttttt cgaagagatc
     9541 cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg gttttggtaa cttcgatctg
     9601 cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat tcccattaca
     9661 gcacggcgcg tggtgacctt cagacccggg cagaagttaa aaagccgggt cgaaaacgct
     9721 tcgcccaaag acgagtaatc ttatctaact aaaaaggccg ctctgcggcc ttttttcttt
     9781 tcactgtcga agagtcaccg taaaatcaac gccatgacac ttcagcagaa cggataccat
     9841 gctgacactt gcccgccaac aacagcgaca aaatattcgc tggttattat gcctgtcagt
     9901 tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt gaacaatgga tctcgccagg
     9961 tgactggttt tctcctcgtg gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct
    10021 ggctgtattg ctggttggtg cggcgctggc tatatccggc gctgtaatgc aggcgttgtt
    10081 tgaaaatcct caggcagaac ctggactact tggcgtctct aacggcgcag gcgtggggct
    10141 tatcgccgcg gtattgcttg ggcaagggca actccccaac tgggcgctag ggctgtgtgc
    10201 gattgctggc gcgcttatca tcactttaat actcttacgt ttcgcccgtc gtcatctttc
    10261 gaccagtcgg ttattgctgg ctggcgttgc attagggatt atctgtagcg cactaatgac
    10321 gtgggctatc tacttttcca cctctgttga tttacgtcag ttgatgtact ggatgatggg
    10381 cggttttggc ggcgtagact ggcggcaaag ctggctgatg ctggcattga tccccgtgtt
    10441 gttgtggatc tgttgtcagt ccaggccgat gaatatgtta gcacttggcg agatctcggc
    10501 gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg ctggtagcag cgaccggctg
    10561 gatggttggc gtcagtgtgg cgctggcggg tgctatcggc tttattggtc tggtgatccc
    10621 acatattctt cggttgtgtg gtttaaccga tcatcgcgta ttacttcccg gctgcgcgct
    10681 ggcaggggcg agcgcattgc tgctggccga tattgtagcg cgcctggcat tagctgccgc
    10741 agagctacct attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta tctggttatt
    10801 gttaaaagca ggacgttagc cgcaaaaaga cggtctatga ttaaaagcta agatttttac
    10861 tgaccacacc caggagaaac gatgcaagat tccattctga cgaccgtagt gaaagatatc
    10921 gacggtgaag tgaccacgct ggagaagtac gccggtaatg tgctgttgat tgtcaatgtc
    10981 tcctcaaagt gtggcttaac gccgcaatat gagcagttgg agaatattca gaaagcctgg
    11041 gccgatcgag gttttgtggt gctgggattc ccgtgcaacc agtttctgga acaagaaccg
    11101 ggcagcgatg aagagattaa aacttactgt accaccacat ggggggtgac gttcccgatg
    11161 ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc tgtatcaaaa attgattgcc
    11221 gcagcgccga ccgcagtcgc gccggaagag agcggattct atgcccgtat ggtcagcaaa
    11281 ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt ttgagaaatt tttagttggc
    11341 agggacggat tggtcatcca gcgtttttcc ccggatatga cgccggaaga tcccattgtg
    11401 atggaaagca ttaaactggc gttggcaaaa taatgtctat tgtgatgcag ttacaggatg
    11461 ttgcggaatc tacccgcctg gggccgcttt ctggcgagat tcgggctggg gagatcctgc
    11521 acctggtggg gccgaatggc gcgggtaaga gtaccttact ggcgcgaatg gccggaatga
    11581 ccagcggtaa gggaagcatt cagttcgcgg ggcaaccact ggaagcatgg tccgcaacaa
    11641 aactcgcgtt gcatcgcgcc tatctttcac aacagcagac gccgccgttt gcaatgccgg
    11701 tctggcacta cctgacactg catcagcacg ataaaacgcg taccgaacta ctgaatgatg
    11761 tcgcaggggc gctggctctt gatgacaaac tcggacgtag caccaatcaa ctttccggcg
    11821 gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca aatcacacca caagccaatc
    11881 ccgcaggcca attgctgctt cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa
    11941 gtgcgttaga caaaattctg agcgctctgt gtcagcaagg actggcgatt gtgatgagca
    12001 gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa
    12061 aaatgctggc cagtggacgc agagaagagg tgctcacgcc gccaaatctg gcgcaggcct
    12121 atgggatgaa ttttcgccgt ctggatatcg aaggtcacag aatgctgatt tcgaccatct
    12181 gatttcatta gtctcttgaa aatcgggtaa ttacgacgct aaattaatgc aagaataaaa
    12241 aacagaggaa ccgtacggaa tgcgtttctg ccttattttg atcacagcac tgtttctggc
    12301 cgggtgtagc caccataaag caccgccgcc aaatgccaga ctttctgatt cgattaccgt
    12361 tattgccggt ttgaacgacc agctacaaag ctggcatggc acgccgtatc gttatggtgg
    12421 catgacgcgg cgcggtgtgg actgttcggg atttgtggtt gtgacgatgc gcgatcgttt
    12481 cgatttgcag ctgccccgag aaaccaaaga acaagcctct atcggcacgc aaattgataa
    12541 agacgagttg ctgcctggtg acctggtctt tttcaaaacg ggttccggac aaaatggttt
    12601 gcatgtaggt atttacgata ccaacaacca atttatccac gcctctacca gcaagggagt
    12661 gatgcgttcc tcacttgata atgtctattg gcagaaaaat ttctggcagg cgagacgaat
    12721 ctagtatgtc gaaaattgga cggcgatgat acatcgccgt ccatttactc cagaatacag
    12781 tagtattttg ca
//
LOCUS       DAZUGK010000130        12492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZUGK010000130 DAZUGK010000000
VERSION     DAZUGK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.131"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..173
                     /locus_tag="ACHY5W_003219"
     CDS             <1..173
                     /locus_tag="ACHY5W_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFS4448079.1"
                     /translation="DKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNENQKVEFSIEQG
                     QRGPAAANVVTL"
     gene            184..372
                     /gene="ymcF"
                     /locus_tag="ACHY5W_003220"
     CDS             184..372
                     /gene="ymcF"
                     /locus_tag="ACHY5W_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4448080.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            347..577
                     /gene="ymcE"
                     /locus_tag="ACHY5W_003221"
     CDS             347..577
                     /gene="ymcE"
                     /locus_tag="ACHY5W_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFS4448081.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            567..740
                     /gene="gnsA"
                     /locus_tag="ACHY5W_003222"
     CDS             567..740
                     /gene="gnsA"
                     /locus_tag="ACHY5W_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFS4448082.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(789..1862)
                     /gene="yccM"
                     /locus_tag="ACHY5W_003223"
     CDS             complement(789..1862)
                     /gene="yccM"
                     /locus_tag="ACHY5W_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4448083.1"
                     /translation="MTEKKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFLARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1934..4678)
                     /gene="torS"
                     /locus_tag="ACHY5W_003224"
     CDS             complement(1934..4678)
                     /gene="torS"
                     /locus_tag="ACHY5W_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFS4448084.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLTTEMADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRVASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4761..5789
                     /gene="torT"
                     /locus_tag="ACHY5W_003225"
     CDS             4761..5789
                     /gene="torT"
                     /locus_tag="ACHY5W_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFS4448085.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAVEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5762..6454)
                     /gene="torR"
                     /locus_tag="ACHY5W_003226"
     CDS             complement(5762..6454)
                     /gene="torR"
                     /locus_tag="ACHY5W_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFS4448086.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6584..7756
                     /gene="torC"
                     /locus_tag="ACHY5W_003227"
     CDS             6584..7756
                     /gene="torC"
                     /locus_tag="ACHY5W_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFS4448087.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7756..10302
                     /gene="torA"
                     /locus_tag="ACHY5W_003228"
     CDS             7756..10302
                     /gene="torA"
                     /locus_tag="ACHY5W_003228"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFS4448088.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCCRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10299..10898
                     /gene="torD"
                     /locus_tag="ACHY5W_003229"
     CDS             10299..10898
                     /gene="torD"
                     /locus_tag="ACHY5W_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFS4448089.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFATRCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(11050..11355)
                     /gene="cbpM"
                     /locus_tag="ACHY5W_003230"
     CDS             complement(11050..11355)
                     /gene="cbpM"
                     /locus_tag="ACHY5W_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFS4448090.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11355..12275)
                     /gene="cbpA"
                     /locus_tag="ACHY5W_003231"
     CDS             complement(11355..12275)
                     /gene="cbpA"
                     /locus_tag="ACHY5W_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFS4448091.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2984 a         3223 c         3314 g         2971 t
ORIGIN      
        1 cagataaagg ttttggcttt atcactcctg atgatggcag caaagacgtt ttcgtccatt
       61 tcaccgccat ccagagcaat gaattccgca cgctgaacga aaatcagaaa gttgaatttt
      121 ctattgagca ggggcaacgt ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc
      181 gttattactc aacatctcca tttccgctgt ccatgttgtc atggttcaca gtaccgcaca
      241 tcagcattcg atgtgacgga gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg
      301 acgatgatca cctttgataa cgtcgcgctg caaatacgca ctgaccatgc gccgctggat
      361 ttcacaaaat aatatcaggc tccctcgtgg agcctttttt atatctgcct tatttttctt
      421 caacgctgta tgtatagtaa gcgataacct gttgattatt gaatctttcg gggagatggc
      481 ttataacatt tcttacctga ccagggtacc gggaaccaac accttactgg cgtgttgctg
      541 tctttcaaga ccagacgagg ttaacagtga atattgaaga gttaaaaaaa caagccgaaa
      601 cggaaatcgc cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag
      661 aagtctctga aattcgcttc accgcacgag aaaaaatgac cgggcttgaa agttatgatg
      721 tcaaaatcaa aataatgtga ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg
      781 ttgctaattt agtgaccaat cattggcgct tgtggaatta agcgtcggta caattcctcc
      841 ggcaccgggc tttgccatac tcccgcatac attgcgtaac caatcaccgc aaacataatc
      901 cccagaacca gtagtgtcat taaccagcca gacaacgcaa aggctttttt atttgccgca
      961 ggtttttgca gtgaaaaggt caatgttgag gctaccggac atgactctac gcaagtcata
     1021 cagccggtac attccactgt tcgtacctga attaatttat cgaccgggat ccgtgatggg
     1081 caattttttg cgcatttgcc acaatcgata caactttcgg cattgcgacg aatcttaaac
     1141 ggcgataata gcgaaaccac gcccatcagc gcgccatatg ggcaaagata acgacaccag
     1201 gcatggcgaa taaacaggct ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc
     1261 cccatatgac gaaagaaatc gagcatttta acgtccatca ccacgctgta gggcgacaac
     1321 ataaaatagt gaatcgcctg agcaggcatc aataacgcga tatagagaaa aaaactcaac
     1381 agcaaatact ttacaccgcg cagaggaata tccagccagc ggggaaggac aaattgccga
     1441 ccaaacagtt tgttaccgag atcgccgatt aattcagaaa gcgtaccaac cgggcataac
     1501 catgagcaaa aggccttttt gagtaataga ctgatgacga taaaagcgac caataacagc
     1561 atcgcggcgg cgtggacgga cggtaactga cctgttgcaa ggctatattt cagattcatc
     1621 agaccggcaa tcggtagcca gccttcgata cctcccggtc tggcgagaaa tgtcgtacta
     1681 cctgccgttt cgtaatagcg cacccaatac cagaacgtga tggcaatata aatattcatt
     1741 gccaacagca ataattgcgt cgctttacgc caggtcgtgg cattgcgcca gttattccac
     1801 ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc gggttctttt tttctctgtc
     1861 atgattttgc cagtccgtta agttgcatac caaatgccac tattctagtt attcttaact
     1921 ggctgatatt gattcaaatc gcgttcaggt ctttcttatg caaccatgct tccagagcgg
     1981 caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc tgctccagtt
     2041 gtgcgcatag ctggctggcg acacgcattc ccagactcga gcaactgctt tttagctgat
     2101 gcgcggcacg ctttattttc tcgctatcct gactggcgcg ggcaatgtcg atttcatcga
     2161 gaagcggcag ggcatgttgt ttaaataatg ccagccattc gtggatcttc tctgtcccca
     2221 ttaactgagc gtcttcattg agttgcgaga catccagtgg tagatcgtta ttggcttgca
     2281 gttggagata gtgcgccagt aactgaccga gcacttcacg cggcaccggt ttagggataa
     2341 tcccgcgaaa tagtgaactg gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa
     2401 agccaatcaa aaccagcgac ggatattgct gtgccagttg ccgggcaagg gtaatgccgt
     2461 cgatatccgg cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg
     2521 tctctaaagc ctgcgcggca ttgccaatag caacaacctg cgcaccactg gtgttcagca
     2581 tctcaacggt aattcgctgg gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt
     2641 caagacgcac cgcctgattg actgtttttg gcacgggtgc cgtggcaaca cgtaacggca
     2701 agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcactcagt tcgccgccca
     2761 tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc
     2821 ttacctgcac aaatggctgg aagatttctg ccagtttcgc gggatcaata ccgcagccgc
     2881 tgtcttccac ttcgaccagc cattgctcgc catcagtgcg gctacgtaaa acgatctgcc
     2941 cttcgtcagt aaaacgtagg gcgttgctca acaggttggt tataacctga cgaatacgtc
     3001 gcggatcgcc cattaacgca gtcggtacat cgtcggccat ttcagttgtc aggcgaattg
     3061 gccgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcagc gggcgcggtt
     3121 caaagggttc atcgctgacc gaaacattct tgccacctgc ttcgatagcg gaataatcga
     3181 gaatatcgtt gaggatggtc agcaacgatt cgccggagtc ggtaattgcc cgcaaatcat
     3241 cacgctgggc gttaagtgcg gggttatctg ccagcagttg agcagtgccg agaataccgt
     3301 acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt gcctggctgg
     3361 ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg
     3421 tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg attcagtgca tgaacactgc
     3481 tgcgaaacgc atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg
     3541 ggaaagggga gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa
     3601 gtggacgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt gcgcaaagtg
     3661 aaaccatccc cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgcg
     3721 ccagtccatg ctgattgcgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa
     3781 actgcgcgaa ctgggcgata ttattttggg cgagagtttg caggtgattg ctgatttcac
     3841 tgtcctgctg gaacagcgcc aacagatcgc tatattggct aacggtagtt aacgttgttg
     3901 cgacctgagc acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca
     3961 ccgcattatt gagctgcttt tccagcgttg gtgcattttt ctggatctgc tccagcccca
     4021 gattcatcac catttgctgc acccgcaggg cgctaaggcg cagttcattc atctggttaa
     4081 catactcaag atcgatatca atcagccgat cgagtgcact ttctgcggcc tgacgctgat
     4141 cttgttcgat caaatcgtaa atcccggcct gggtcgctcc ggcggaggtt gccgcattat
     4201 tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc ggcgactatc tgctgactga
     4261 gtcgctgttg ttgctggcgc agttgcaaac gctgccccac cagttcccct tgctgacgta
     4321 acgaacggga gatctcctgc tcctgttgtt caatagcggt ggtatcaaaa ccttgttccc
     4381 gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt cgcccctgcg
     4441 cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa agttcccagg
     4501 cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc tgagtattgt
     4561 ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta ctggtcaggg
     4621 ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt aaattcacgg
     4681 tcggtgtact taaggtgaaa aaggttgagt cgcaaagcgg aatgcatcta gcataaagcc
     4741 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
     4801 tgccggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
     4861 aagcctctat gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt tatccgagcc
     4921 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
     4981 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
     5041 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
     5101 catttcccga tctgcaaaag caggtagcaa gtctgccggt gatcgaactg gtaaatgcta
     5161 ttgatgctcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
     5221 ctgggcgata tctggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ttgatgcccg
     5281 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
     5341 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgta
     5401 acctgttgca ggagatgctg gaacgccatc cagaaatcga cgtcgttgcc ggaacggcca
     5461 ttgcggtaga ggcggcaatg ggggaagggc gtaacctgaa aacgccgctt accgtggtgt
     5521 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     5581 ccagcgatca aatggtctgg cagggggaac tggcggttga gcaggccatc aggcaattac
     5641 agggacaatc ggtatctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     5701 ccgaccgcga acatatccgc cgctcgctgt cgccaggggg atttcgtccg gtctattttt
     5761 atcagcacac atcagcggct aagaaataac cttcgccatg ttgcgtcacc agtaaatccg
     5821 cgctgagttt atggcgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     5881 ccacccgacg cgcagagagc atacgcagca gacgttcacg gctgagaatt tctcccggat
     5941 tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcaccat
     6001 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagttgt
     6061 cctgagtgag cggttgaact tgtcgcgcga ggtcgattcg ccagagcaga ttttttaccc
     6121 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     6181 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     6241 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggcaag ttgatatcca
     6301 gcagaattaa atccaccggc tgattctgca taatttcccg cagcccggca ccgctcgccg
     6361 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     6421 gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     6481 atctgttcat attctgccgt aagccattca tcctgaccag tgccgctgtt catatttgct
     6541 cattaagatc gcttcactaa accataattt tacaggggct attatgcgga aactctggaa
     6601 cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcta ttgggattgt
     6661 gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca gcacaaccga
     6721 attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac agtcggtgca
     6781 tttccagaac gcctccggcg tgcgagctga atgccatgac tgtcatatcc cgccggatat
     6841 tccaggcatg gtgaagcgca aactggaagc gagcaacgac atttaccaga cctttattgc
     6901 tcactccatt gatacacctg aaaaattcga agccaaacgc gcggaacttg ccgagcgtga
     6961 atgggcgcga atgaaagaaa acaactcggc aacctgccgc tcctgccata actacgacgc
     7021 gatggatcat gcgaagcagc atcctgaagc ggcacgtcag atgaaggtgg cagcgaaaga
     7081 taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg atatgagtag
     7141 cggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgacagtg gtgacacgct
     7201 gtactctatt gatattaagc cgatttatgc ggcgaaaggc gataaagaag cctctggttc
     7261 tctgctgcct gcttcggaag tgaaagtcct taaacgtgac ggcgactggc tgcaaattga
     7321 aattaccggc tggacagaaa gcgccggacg tcagcgtgta ctcacccaat tcccaggtaa
     7381 acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtgaaaa cgctggagaa
     7441 aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggccaccg cgtggatgaa
     7501 gaaaggcgac atggtgaacg atatcaaacc gatctgggct tatgcggatt cgctgtacaa
     7561 cggcacctgt aaccagtgcc acggcgcacc ggaaatctcc cactttgacg ctaacggttg
     7621 gatcggtacg ctcaacggca tgattggctt taccagcctc gataaacgtg aagaacgcac
     7681 cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaggctc acggcgataa
     7741 gaaggaagaa aaataatgaa caataacgat ctctttcagg catcacgtcg gcgttttctg
     7801 gcacaactcg gcggcttaac cgtcgccggg atgctggggc cgtcattgtt aacgccgcgc
     7861 cgtgcgactg cggcgcaagc ggcgactgag gctgtcatct cgaaagaggg tattcttacc
     7921 gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg gtcgctttgt ggcggcaaaa
     7981 ccgttcgaac tggataaata tccgtcgaaa atgatcgccg gattgccgga ccacgtacac
     8041 aacgcggcgc gtattcgtta tccgatggta cgcgtggact ggctgcgtaa gcgccatctg
     8101 agcgacacct cccagcgcgg tgataaccgt tttgtgcgtg tgagctggga tgaagccctc
     8161 gacatgttct atgaagaact ggaacgcgta cagaaaactc acgggccgag tgccttgctg
     8221 accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat gctggcgaaa
     8281 gctattgcct tgcatggtaa tagcgttggt acgggcggag attactctac tggtgctgcg
     8341 caggtgatcc tgccgcgcgt agtcggttcg atggaagtgt atgaacagca aacctcctgg
     8401 ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctccgattt gctgaaaaac
     8461 cagcaagcga actggtggtg cccggatcac gatgtttatg aatattacgc gcagttgaaa
     8521 gcgaaagtcg ccgccggtga aattgaggtc attagcatcg atccggttgt tacatccacc
     8581 catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca aactgacgtg
     8641 ccgctgcaac tggcgctggc gcatacgctg tacagtgaaa acctgtacga caaaaacttc
     8701 ctcgctaact actgtgtggg ttttgagcag ttcctgccgt atctgctggg tgagaaagac
     8761 ggtcagccga aagatgccgc atgggctgaa aaactgaccg gcattgatgc cgaaaccatt
     8821 cgtggactgg cgcggcagat ggcggcgaac agaacgcaga ttattgctgg ctggtgcgta
     8881 cagcgtatgc agcacggtga acagtgggcg tggatgattg tggttctggc ggcgatgctg
     8941 gggcaaattg gcctgccagg tggtggcttt ggttttggct ggcactataa cggcgcaggc
     9001 acgccggggc gtaaaggcgt tattctgagt ggtttctccg gctctacgtc gattccgcct
     9061 gttcacgaca acagtgatta caaaggctac agcagcacca ttccgattgc ccgttttatc
     9121 gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt aaaactgccg
     9181 ccgctgaaaa tgtgtatttt tgccggaact aacccattcc atcgccatca gcagatcaac
     9241 cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa ccagtggacc
     9301 tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga gcgtaacgat
     9361 ctcgaccagt acggcaacca ctccaaccgt ggcattatcg ccatgaaaca ggtcgtgccg
     9421 ccgcagttcg aggcgcgcaa cgactttgat attttccgcg agctgtgctg ccgctttaat
     9481 cgcgaagaag cctttaccga agggctggac gaaatgggct ggctgaaacg catctggcag
     9541 gaaggtgttc agcaaggcaa aggacgcggc gttcatttgc cagcgtttga tgacttctgg
     9601 aataacaaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca ccaggcattc
     9661 cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga gatctattcg
     9721 aaaaccatcg ccgatatgaa ctacgacgat tgtcaggggc atccgatgtg gtttgagaaa
     9781 atcgaacgct cccacggtgg gcctggctcg cagacgtatc cgttgcatct gcaatctgtg
     9841 catccggatt tccgacttca ctcgcagtta tgtgagtcgg aaactctgcg tcagcaatat
     9901 acggtagcgg gtaaagagcc agtgttcatt aacccgcagg atgccagcgc gcgcggtatt
     9961 cgtaacggtg atgtggtacg cgtctttaac gctcgcggtc aggtgttggc aggggcagta
    10021 gtttctgacc gctatgcacc cggcgtggcg cgaattcatg aaggggcatg gtacgatcct
    10081 gataaaggcg gtgagcctgg tgcgctgtgc aaatacggta accccaacgt gttgaccatc
    10141 gacatcggta cttcacaact ggcgcaggcg accagtgcgc acactacgct ggtggaaatt
    10201 gagaagtaca acggaacagt ggatcaggtg acggcgttta acggccccgt ggagatggtg
    10261 gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat gaccacgctg acagcacaac
    10321 agattgcctg tgtttacgcc tggctggcgc agttgttctc ccgtgaactg gacgatgaac
    10381 aactgacgca gatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg aaaagcgaac
    10441 cgccgctcac tgcggcggtg gatgagctgg aaaaccgtgt tgccactctg acagtacgtg
    10501 acgatgcccg tctggaactg gccgctgact tttgcggcct gtttctgatg actgacaaac
    10561 aagcggcgct gccgtatgca tcggcctaca aacaggacga gcaagagatt aaacgcttgt
    10621 tagttgaggc agggatggaa accagcggca atttcaacga accggcagat catctggcga
    10681 tctatctcga gttgttgagt catctgcatt tttcgctggg agaggggacg gttcctgcgc
    10741 gaagaatcga cagtttacgg caaaaaacac tcacggcgct gcggcaatgg ttaccagagt
    10801 ttgctacgcg ttgccatcag tatgacagct ttggttttta cgcagcacta agccagttat
    10861 tgctggtgtt agtggagggc gaccaccaaa acagataacg tcgtttgtgc gcctgaaaag
    10921 atgcgtttag cgtcgcatca ggcattaggg caccgccgcc ggatgcggca tgaacgcctt
    10981 aaccggccca caggaaaagt actctttgta gaccggttaa gatgcgtcat cacatccggc
    11041 aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgcagca ggcggttttc
    11101 ctgcttcagg tgcgcaatat catccattaa cgtcagcgcc accgcgatcc ccggccagtc
    11161 cagagccagt tcatgacgca ggcgcaccgc gcgttgcacc acgatggcgg catggtcgtc
    11221 aaatacccag gttgtttcct gaatctcacg cggttcaacc acccccaaac cgacaatttc
    11281 attcaattcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag taaaagtcac
    11341 cgtaacatta gccattatgc tttcccccaa tctttacgtg gatcaaaaga cgactgggcg
    11401 tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg catcacgatt
    11461 ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt gcctttaacg
    11521 cgcaatcgtt gcccggcctg gctgccaggc gggatagtca gcaaaatgct ttctttcagt
    11581 gttggaacgg tgactttagc acccagcgcc gcttcccacg ggctaaccgg caccacaatt
    11641 tccagatcct ggccgacaat atcaaacagc ggatgtggcg caatatgaat caccagccac
    11701 aggtcgccat ttggaccgcc gttttcgccc ggcgtcccct ggcctttcag gcggatacgt
    11761 tgaccattgc cgacgcccgc cgggatcttc acattcagcg ttttcgggat ttcctgttcg
    11821 atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt atgctcagta
    11881 agcgtttctt cgaggaatac cgccacttcg atttcaatat cgtggccgcg tgtggcgggg
    11941 cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc gtcaaaatct
    12001 tcggcgttaa aactctgtcc gtcgccatga tggaactgac ggttaaattg cggatcgttg
    12061 cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa cacttcccag
    12121 gcttcagcga cctctttgaa gcgggcttcg gcatccggtt ctttgctgac atcaggatgg
    12181 tatttgcggg caagtcgacg ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc
    12241 acgcccatga tggcgtaata atcctttaat tccatagcgt tatctcgcgt aaatcaacac
    12301 aaattgaagg aacccctgta aggtaactcc tataagtgta gggtaatcct caaaatttca
    12361 tatgccaaca cagaatatgt tattgaaatc atcgcggaga ggaggtcgcc atcaagatgg
    12421 gttgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata gttgaaccag
    12481 gcactttaag tt
//
LOCUS       DAZUGK010000131        12442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZUGK010000131 DAZUGK010000000
VERSION     DAZUGK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.132"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(574..658)
                     /locus_tag="ACHY5W_003232"
     tRNA            complement(574..658)
                     /locus_tag="ACHY5W_003232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(622..624),aa:Leu,seq:caa)
     gene            854..1873
                     /gene="ahr"
                     /locus_tag="ACHY5W_003233"
     CDS             854..1873
                     /gene="ahr"
                     /locus_tag="ACHY5W_003233"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFS4448092.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTIGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVVPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2003..3505
                     /gene="yjgR"
                     /locus_tag="ACHY5W_003234"
     CDS             2003..3505
                     /gene="yjgR"
                     /locus_tag="ACHY5W_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFS4448093.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3666..4748)
                     /gene="lptG"
                     /locus_tag="ACHY5W_003235"
     CDS             complement(3666..4748)
                     /gene="lptG"
                     /locus_tag="ACHY5W_003235"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS4448094.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4748..5848)
                     /gene="lptF"
                     /locus_tag="ACHY5W_003236"
     CDS             complement(4748..5848)
                     /gene="lptF"
                     /locus_tag="ACHY5W_003236"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS4448095.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(5911..5961)
                     /gene="ytgA"
                     /locus_tag="ACHY5W_003237"
     CDS             complement(5911..5961)
                     /gene="ytgA"
                     /locus_tag="ACHY5W_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFS4448096.1"
                     /translation="MSIIILATISTTQENK"
     gene            6115..7626
                     /gene="pepA"
                     /locus_tag="ACHY5W_003238"
     CDS             6115..7626
                     /gene="pepA"
                     /locus_tag="ACHY5W_003238"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFS4448097.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            7980..8423
                     /gene="holC"
                     /locus_tag="ACHY5W_003239"
     CDS             7980..8423
                     /gene="holC"
                     /locus_tag="ACHY5W_003239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFS4448098.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8423..11278
                     /gene="valS"
                     /locus_tag="ACHY5W_003240"
     CDS             8423..11278
                     /gene="valS"
                     /locus_tag="ACHY5W_003240"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4448099.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTDAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11334..>12442)
                     /gene="yjgN"
                     /locus_tag="ACHY5W_003241"
     CDS             complement(11334..>12442)
                     /gene="yjgN"
                     /locus_tag="ACHY5W_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFS4448100.1"
                     /translation="NVLLTIITLGIYLPWALMKCKRYLYANMEVNGQRFSYGITGGNV
                     FVSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLLIFMAAKGLRYQALMTSLNGVR
                     FSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIMSVVLMAIVGIVS
                     IGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAFLALLPFLAVAGYII
                     FDQILNEYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFM
                     SNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLL
                     GDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3192 a         3175 c         3303 g         2772 t
ORIGIN      
        1 tgatggataa tccgatattg gtcgcattta atggcatggt agcaaagttt tcatcacata
       61 cctcctttgc accagaataa acgccagaga tctgaaattg cgagtacact acagaaattg
      121 tactgccggt cccatcagca taaaaccata gttacacagg taatcatcat tattcgccaa
      181 gataataaat attattcggt agaatagcgt tctcaggtgg tcagtataat tgtcttcaac
      241 aagatgtctg cgcatctcgt ctgctagccc tacataactg ctctcgaccg aaactattta
      301 ttgaatgttc accaaggtgg gcgtggagac aaaatgacgt cttttgatat tagctacctg
      361 agccacatgg tcaaaacgtt caagtagcat agcgaaacca ccctgcccac gtaatgtgga
      421 cgatgcagct tccagccaga gcaaacttgg gaccatcgac tacaaaatga agaatactga
      481 actgcttttc tggcaggcct atatactgca cggatatggg tgatttcaga cacaaaaaaa
      541 gccgcccttg agcgactcga tttgcatacg gtgtggtgcc gaaggccgga ctcgaaccgg
      601 cacgtatttc tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac
      661 tgaagaggta tgcggaaaac gttgtggatt atacctgtcg cacgccacca tgcaagcgcc
      721 agcttgctcg cacctcgcta agtgttgaaa ttttcagcat tacactcagc gatgtgtaac
      781 ctttgtcaca ctccaggcac cccgccctgc catgctctac acttcccaga caacaccaga
      841 gaaggaccaa aaaatgtcga tgataaaaag ctatgccgca aaagaagcgg gcggcgaact
      901 ggaagtttat gagtacgatc ccggtgagct gaagccacaa gatgttgaag tgcaggtgga
      961 ttactgcggg atctgccatt ccgatctgtc gatgatcgat aacgaatggg gattttcgca
     1021 atatccgctg gttgccgggc atgaggtgat tggtcgcgtg gtggcgctcg ggagcgccgc
     1081 gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt ggctggacgg cacgcagctg
     1141 cgggcattgc gatgcctgta ttagcggtaa tcagatcaac tgcgagcaag gtgcggtgcc
     1201 gacgattatg aatcgcggtg gctttgccga gaagttgcgt gcggactggc aatgggtgat
     1261 tccactgcca gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg gcggtatcac
     1321 ggtctttaaa ccactgttga tgcaccatat cactgctacc agccgcgttg gggtaattgg
     1381 tattggcggg ctggggcata tcgctataaa acttctgcac gcaatgggat gcgaggtgac
     1441 agcctttagt tctaatccgg cgaaagagca ggaagtactg gcgatgggtg ccgataaagt
     1501 ggtgaatagc cgcgatccac aggcactgaa agcactggcg gggcagtttg atctcattat
     1561 caacaccgtc aacgtcagcc tcgactggca gccttatttt gaggcgctga cctatggcgg
     1621 taatttccat acaatcggtg cggttctcac gccgttgccg gttccggcct ttacgctgat
     1681 tgcgggcgat cgcagtgtct ctggctctgc taccggtaca ccttatgagt tgcgtaagct
     1741 gatgcgcttt gccgcccgca gcaaggttgt gccgactacc gaactgttcc cgatgtcgaa
     1801 aattaacgac gccattcagc atgtacgcga tggtaaagcg cgctaccgag tggtcctgaa
     1861 agccgacttc tgatcccttt gccgggtgcc tgtcgcccgg cacttaaatc tctttggaag
     1921 gcgtctcgca atctgctgaa tttagcggtg aagagtgcgc agccgttgcc tatactccag
     1981 gcaggaaact ggaggaaatc tcatgagcga acccctgtta attgcccgca cgccggacac
     2041 agaactattt ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg
     2101 gacgggtaaa actgtcacgc tgcaaaaact ggcggagtct ctatcggaaa tcggtgttcc
     2161 ggtgtttatg gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc
     2221 ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat gactggcaac cgcatgccaa
     2281 tccggtggtg gtgtgggata tctttggcga gaaaggccac ccggtgcggg cgacggtttc
     2341 ggatctgggg ccgctgttgc tggcgcggct gttgaatctc aacgatgtgc aatctggcgt
     2401 gctgaatatc attttccgca ttgctgacga tcaggggctg ttactgctcg actttaaaga
     2461 tctacgggca attacccagt acatcggcga taacgccaaa tctttccaga atcagtacgg
     2521 taatatcagt agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc tggaacagca
     2581 aggcgcagca cacttctttg gtgagccgat gctggatatc aaagactgga tgcgcaccga
     2641 tgccaacggt aaaggcgtta ttaatatcct cagcgccgag aagctttatc agatgccgaa
     2701 actgtacgcc gccagcctgc tgtggatgat ttcagagttg tatgaacaat tgccggaagc
     2761 gggcgatctg gagaagccaa aactggtgtt tttcttcgac gaagcacatc tgctgtttaa
     2821 cgatgcaccg caggtactgc tggataagat tgagcaggtg ataaggctta ttcgctcaaa
     2881 aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat attccagata atgtgctcgg
     2941 ccagctcggt aatcgcgttc agcacgcttt gcgggctttt acgccaaaag atcagaaagc
     3001 agtgaaggca gcggcgcaaa ccatgcgggc taatccggcg tttgataccg aaaaggcaat
     3061 ccaggaactg gggacgggcg aagcgttgat ctcgtttctc gatgcaaaag gaagtccttc
     3121 tgtggtggaa cgggcgatgg tgattgcgcc ttgttcgcgg atgggaccgg tgacggaaga
     3181 tgagcgcaat ggcttgatta atcactctcc ggtgtatggc aagtacgagg atgacgtgga
     3241 ccgggaatcc gcctatgaga tgttgcaaaa aggctttcag gccagtaccg agcagcaaaa
     3301 taatcccccc gcgaaaggga aagaggtagc ggtagatgac ggcattcttg gtggattgaa
     3361 ggatattttg ttcggcacta ccggaccacg cggcgggaag aaagatggtg ttgtgcaaac
     3421 aatggcgaaa agcgccgccc gccaggtgac gaatcagatt gtgcgtggaa tgttggggag
     3481 tttgctgggg gggagaaaaa ggtaagccag gtgcgatttg tctttgcctg atgcgacgct
     3541 ggcgcgtctt atcatgccta caccactgca atatattgga ttttcataat ttttcaggaa
     3601 tgaacgaagc acactttgct aacaataata aagggagctt tcgctccctt tattcgttca
     3661 ttcggttacg attttctcat taacagccac aggctgatta agaagaagct ggcgcttggc
     3721 aacagtgcgc cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc gaagatctgg
     3781 tccagtacgt agaagacaaa accgaagcta ataccggtga ccacacgcac acccatcggt
     3841 acgctacgca gtgggccaaa gatgaacgac agcgccatca gcatcatcac cgccacggat
     3901 agcggctgga agattttgct ccacatgttg agctgataac gtccggcatc ctgaccgctc
     3961 gacttcagat acttcacata gttgtgcaaa ccgctgatgg agagtgcatc cgggtccagc
     4021 gccaccacgc ccagtttgtc tggcgtgagg ttggttttcc aggtgccgct cactgtctgc
     4081 gaaccggtga tctgtttcgg attggtcaga tcagattcat caacctgcga cagacgccag
     4141 actttatgtt ccgggtcaaa cttcgcagta gcagcatagc gtacggattg cagacgacga
     4201 ttctcgttaa aggcataaat gctgatgcca cctaactctt cgtctccttt aacccgctca
     4261 atgtagacga agttgttgcc atctttcgcc cataagcctt gctgggtaga gagcaacgag
     4321 ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct gctcgccctg cggcgcgacc
     4381 cattcaccaa tcgccatcgt cagtaagacc agcggaatgg cggttttcat caccgacagc
     4441 gcaacctgca tacgggtgaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc
     4501 agcatcccaa gaccaagcaa cgccccaagc agagccgcca tcgggaagaa tatttgcaca
     4561 tctttcggca cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc
     4621 ccggcttttt tcaactgatc gacaaatttg ataatgcctg acagcgacac cagcatgaac
     4681 agcgtcatca tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa
     4741 ggttgcatca caccgctcct ttacgcgaaa aactggcgcg caggcggcgg accggcacgg
     4801 tgtcccaaag gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc
     4861 acagcgtcgg gtccagctta cctttaccac cgttcgattt cagggaggtc tggatcagga
     4921 agaaaagtag atacagcagc atggctggca gcatcgatag tacgcgtccc tgacgtgggt
     4981 tgaccacgct cagcggtacg accataagtg ccatcataaa cacggtgaat accaacgtga
     5041 tacgccagtt cagttctgcg cgggcacgat cggtgtcagt gttccacaat gtacgcatgt
     5101 ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat
     5161 aatcctggaa gtccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc
     5221 cctggttgag agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg
     5281 ccaccaccac agaaggacgt gcattacctt ttggtcgaat ttgcgcgagg aacacatctt
     5341 tgaaatcgct gccgtcaacg ctttcgatga acagcaccga gctgccatta gtcgcttgct
     5401 ggaattgccc ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt
     5461 catcctgatg acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg
     5521 ccgtgaatac cgcaaggatc attgccgctt tcaccagaac cgctttgctc aggccgcagg
     5581 catgcattac cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga
     5641 ggaacaggct taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg
     5701 agagcaccag attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact
     5761 tttgacagaa gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct
     5821 cccgcaccag atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc
     5881 aggattttag cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt
     5941 cgctaagata attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc
     6001 cgtaataacg ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct
     6061 tgcaattcta tctgtagcca ccgctgttgt ctttaagatt caggagcgta gtgcatggag
     6121 tttagtgtaa aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc
     6181 ttcgaaccac gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac
     6241 atcagcgccc tgctacgtcg gggcgaactg gaaggaaaac cggggcagac attgttgctg
     6301 caccatgttc cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt
     6361 gagctggatg agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat
     6421 actggctcaa tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac
     6481 tactggaaag tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag tttcgatcag
     6541 ctgaaaacga acaagagcga accgcgtcgt ccgctgcgta aaatggtgtt caacgtgccg
     6601 acccgccgtg aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc
     6661 gggattaaag cagcgaaaga tctcggcaat atgccgccga atatctgtaa cgccgcttac
     6721 ctcgcttcac aagcgcgcca gctggctgac agctacagca agaatgtcat cacccgcgtt
     6781 atcggcgaac agcagatgaa agagctgggg atgcattcct atctggcggt cggtcagggt
     6841 tcgcaaaacg aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca
     6901 cgcccaatcg tgctggtggg taaaggttta accttcgact ccggcggtat ctcgatcaag
     6961 ccttcagaag gcatggatga gatgaagtac gatatgtgcg gtgcggcagc ggtttacggc
     7021 gtgatgcgga tggtcgcgga gctacaactg ccgattaacg ttatcggcgt gttggcaggc
     7081 tgcgaaaaca tgcctggcgg acgagcctat cgtccgggcg atgtgttaac caccatgtcc
     7141 ggtcaaaccg ttgaagtgct gaatactgac gctgaaggcc gcctggtact gtgcgacgtg
     7201 ttaacttacg ttgaacgttt tgagccggaa gcggtgattg acgtggcgac gctgaccggt
     7261 gcctgcgtga tcgcgctggg tcatcacatt accggtctga tggcgaacca taatccgctg
     7321 gcccatgaac tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg
     7381 ggtgacgagt atcaggaaca gctggagtcc aattttgccg atatggcgaa cattggcggt
     7441 cgtcctggtg gggcgattac cgcaggttgc ttcctgtcgc gctttacccg taagtacaac
     7501 tgggcgcacc tggatatcgc cggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc
     7561 ggtcgtccgg tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa
     7621 gagtaattgc gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc
     7681 tacacagcac tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg
     7741 ttattgtcgg atgcggcatg aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc
     7801 tgataagacg cggcagcgtc gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg
     7861 ccttatccga cctacacaca gcactgaact cgtaggcctg ataagacaca acagcgtcgc
     7921 atcaggcgct gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata
     7981 tgaaaaacgc gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg
     8041 ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca
     8101 tcgcctgtga agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg
     8161 cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg
     8221 agatcgcctg gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa
     8281 caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag
     8341 attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc
     8401 tgaatacggc aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc
     8461 gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga
     8521 gagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc
     8581 cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac
     8641 cctgtggcag gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa
     8701 gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa
     8761 aatctgggaa tggaaagcgg aatccggcgg cacgattacc cgtcagatgc gccgtctcgg
     8821 caactccgtc gactgggagc gtgaacgctt cacgatggac gaaggcctgt ccaatgcggt
     8881 gaaagaagtt ttcgttcgtc tatataaaga agacctgatt taccgtggca aacgcctggt
     8941 aaactgggac ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc
     9001 gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg
     9061 taaagattat ctggtggtcg cgactacccg tccggaaacc ctgctgggcg atactggcgt
     9121 agccgttaac ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc
     9181 gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac
     9241 cggctgcgtg aaaatcactc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca
     9301 cgcgctgccg atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt
     9361 gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca
     9421 gaaactcgag cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct
     9481 gctggaagaa attaaaccgc acgacctgac cgttccttac ggcgaccgtg gcggcgtagt
     9541 tatcgaacca atgctgaccg accagtggta cgtgcgtgcc gatgtactgg cgaaaccggc
     9601 ggttgaagcg gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta
     9661 cttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca
     9721 ccgtatcccg gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagaaga
     9781 agtgcgcaag gaaaataacc tcggtgccga cgttgccctg cgtcaggacg aagacgttct
     9841 cgacacctgg ttctcttctg cgctgtggac cttctctacc ctaggctggc cggaaaacac
     9901 cgacgcactg cgccagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt
     9961 cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa
    10021 accgcaggtg ccgttccaca ccgtttacat gaccggcctg attcgtgacg acgaaggcca
    10081 gaagatgtcc aaatccaagg gtaacgttat tgacccgctg gatatggttg acggtatttc
    10141 gctgccggaa ctgctggaga aacgcaccgg caatatgatg cagccgcagc tggcggacaa
    10201 aatccgtaag cgcaccgaga agcagttccc gaacggtatt gagccacacg gtactgacgc
    10261 gctgcgtttc accctggcgg cactggcttc taccggtcgt gacatcaact gggatatgaa
    10321 gcgcctggaa ggttaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct
    10381 gatgaacaca gaaggtcagg attgcggctt taacggcggt gaaatgacgc tgtcgctggc
    10441 ggatcgctgg attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga
    10501 cagcttccgc ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga accagttctg
    10561 cgactggtat ctggagctga ccaagccggt aatgaacggt ggaacagacg ccgaactgcg
    10621 cggtactcgc catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat
    10681 cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc
    10741 cgacaccatc atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc
    10801 actggccgac accgaatggc tgaaacaggc gatcgttgcc gtacgtaaca tccgtgcaga
    10861 aatgaatatc gcgccgggca aaccgctgga gctgctgctg cgtggttgca gcgcggatgc
    10921 agaacgtcgc gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc tggaaagcat
    10981 caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc
    11041 agagctgctg atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc
    11101 gaaagaagtg gcgaagatcg aaggcgaaat cagccgtatc gagaacaagc tggcgaatga
    11161 aggctttgtc gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg
    11221 ctatgcggaa gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc
    11281 aaaacacagt gatgaaaacg aaggccggag catgctccgg ccttttttat ctcttacaga
    11341 aatggtaaag aaggcataat tccccgtgaa atactggcta agaagccttt atctggttgt
    11401 tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt attttttgcc
    11461 tgccagtcaa tcatccatat tttcagcagt ggataagcca gaccgcccgt aatcccggat
    11521 atcaccacca acgcacacat gcgataaagc ataccgtggt atgttaaagt tgagcgaaaa
    11581 cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt
    11641 aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat tatcatttta
    11701 cgctgcattt ccataaattg ctgtaaattc tcaatatcat catttgcata cacagaacta
    11761 tcatactcat ttaatatttg atcgaagata atataaccag caacagcgag aaaaggtaat
    11821 aaagctaaaa atgcgagaat ggcatatttt atacaatacg tagtatctaa tttcaccttg
    11881 aaacgatgta taccgaaact ggtattgctc cagagaaaac tcattaccag gctatataaa
    11941 gtaccattaa aaataccaat ggaaacaata ccaactattg ccatcagaac cacagacata
    12001 ataacactac ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagtcccc
    12061 atcccaatgg ccattaaaat cggcaagaaa aaggttaccc accagaaccc tttcaaagag
    12121 caattaaaac taaatcttac gccgttgaga ctggtcatca aggcctgata acgcagtcct
    12181 tttgctgcca taaatataag caaaaccaac agtgacaaag tcaaaacaca gccaacaagc
    12241 ggcatatctg ctgacactgt cattaagatt gcgaaataga aaaaaacaaa aacaagacaa
    12301 ctaacaaaaa cattcccacc agtaattcca taagaaaatc gttgtccgtt aacttccata
    12361 ttagcataga gataacgctt acatttcatt aatgcccatg gtaaatagat acctagcgtt
    12421 ataatcgtta acaacacatt ca
//
LOCUS       DAZUGK010000132        12270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZUGK010000132 DAZUGK010000000
VERSION     DAZUGK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.133"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHY5W_003242"
     CDS             complement(<1..293)
                     /locus_tag="ACHY5W_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4448101.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEG"
     gene            537..860
                     /locus_tag="ACHY5W_003243"
     CDS             537..860
                     /locus_tag="ACHY5W_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFS4448102.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1158..1427)
                     /locus_tag="ACHY5W_003244"
     CDS             complement(1158..1427)
                     /locus_tag="ACHY5W_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFS4448103.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1439..2398)
                     /locus_tag="ACHY5W_003245"
     CDS             complement(1439..2398)
                     /locus_tag="ACHY5W_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFS4448104.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(2489..2565)
                     /locus_tag="ACHY5W_003246"
     tRNA            complement(2489..2565)
                     /locus_tag="ACHY5W_003246"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2529..2531),aa:Arg,seq:tct)
     gene            complement(2579..2655)
                     /locus_tag="ACHY5W_003247"
     tRNA            complement(2579..2655)
                     /locus_tag="ACHY5W_003247"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2619..2621),aa:Arg,seq:tcg)
     gene            complement(2665..2740)
                     /locus_tag="ACHY5W_003248"
     tRNA            complement(2665..2740)
                     /locus_tag="ACHY5W_003248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2705..2707),aa:Ile,seq:cat)
     gene            complement(2781..3839)
                     /locus_tag="ACHY5W_003249"
     CDS             complement(2781..3839)
                     /locus_tag="ACHY5W_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS4448105.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(3991..4188)
                     /locus_tag="ACHY5W_003250"
     CDS             complement(3991..4188)
                     /locus_tag="ACHY5W_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4448106.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4404..4784)
                     /locus_tag="ACHY5W_003251"
     CDS             complement(4404..4784)
                     /locus_tag="ACHY5W_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS4448107.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4803..5792)
                     /locus_tag="ACHY5W_003252"
     CDS             complement(4803..5792)
                     /locus_tag="ACHY5W_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4448108.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(5844..6101)
                     /locus_tag="ACHY5W_003253"
     CDS             complement(5844..6101)
                     /locus_tag="ACHY5W_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448109.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(6098..7498)
                     /locus_tag="ACHY5W_003254"
     CDS             complement(6098..7498)
                     /locus_tag="ACHY5W_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4448110.1"
                     /translation="MTNPTNPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVGRVECRNQGLEKSRALMTDIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(7495..8385)
                     /locus_tag="ACHY5W_003255"
     CDS             complement(7495..8385)
                     /locus_tag="ACHY5W_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4448111.1"
                     /translation="MTNFLNKQYMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDCVYDELRALKVREL
                     LDLAAIARRFQDCEFDNYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMIMIGNCGTG
                     KNHLAVSMAKNIIRHHLATVEITDVMRLTRAVKNTWRHNSERTEDEVIDHFVSLDLLI
                     IDEVGVQFGTPAEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(8403..9296)
                     /locus_tag="ACHY5W_003256"
     CDS             complement(8403..9296)
                     /locus_tag="ACHY5W_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4448112.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNHFSRNGETRFRETVKPDFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFDESWQQSAVPGGGR
                     GKSLPISGFSEQDYGSTNFNW"
     gene            complement(9283..9516)
                     /locus_tag="ACHY5W_003257"
     CDS             complement(9283..9516)
                     /locus_tag="ACHY5W_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448113.1"
                     /translation="MIRYIVNSLYHRYNRCPRVGQWFATSNGHVLRVCLVNTESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(9513..9707)
                     /locus_tag="ACHY5W_003258"
     CDS             complement(9513..9707)
                     /locus_tag="ACHY5W_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448114.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            complement(9704..10366)
                     /locus_tag="ACHY5W_003259"
     CDS             complement(9704..10366)
                     /locus_tag="ACHY5W_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFS4448115.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELIKAIYDAPASF
                     LSSALTHARIMVGWAGEPKGSPVSLIAGTANPVQFTTHEFRSSGGGSFSPIKEAAIMA
                     TVPTSPFLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIGAFHEAETSLNHTAFRRNQWEV
                     RP"
     gene            complement(10475..11182)
                     /locus_tag="ACHY5W_003260"
     CDS             complement(10475..11182)
                     /locus_tag="ACHY5W_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4448116.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHSDVKRSIERLVAKNIIRKP
                     PMAVSEKINNLGFKVQYEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQVR
                     KPMSEIEMVAAMALEAVRQQKRLDKMEEKVSHVAETVEQIKRGTIRDGYAGYRQLVAK
                     TGMSDAKCRNLVNDYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(11179..11430)
                     /locus_tag="ACHY5W_003261"
     CDS             complement(11179..11430)
                     /locus_tag="ACHY5W_003261"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4448117.1"
                     /translation="MKSAIEKAIDIAGGVNALARAVGVKQPSVSRWRKIGLVGVEHVP
                     DVSAVTGIPAHELRPDKPKLFPHPSLAHTEDGVCEGDKQ"
     gene            11566..>12270
                     /locus_tag="ACHY5W_003262"
     CDS             11566..>12270
                     /locus_tag="ACHY5W_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015840118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4448118.1"
                     /translation="MLRTLADRLNFAMYEMGMSQAQLAKAANMAQPTIWRIASGNARG
                     TTKIVDLANALGVTPEWLSSGVGSMRAENKKPSIPPKSEWGKIESWDEHTPLSDDEVE
                     VPFLKDIEFACGTGKVISEDHNGLKLRFSKATLRRIGANSDGSGVLCFPATGNSMEPI
                     IPDGTTVAIDTNNKRIVDGKLYAIGQDDGCGGQLKRIKQLHRRPGGKLIIRSYNSDEY
                     PDEETSIDKVDIIGRLF"
BASE COUNT         3088 a         3289 c         2796 g         3097 t
ORIGIN      
        1 ccttcaccat acgacatgcc gtttgactgc cctgccagaa ccacaacaaa gtaatacccg
       61 gggtcgctgg tggcgctgat tactgcacct tctccacctg tcggcttcac cacaacaggt
      121 gtgctcacat caccttctgc gacaatcgcc tgaataagtg ctgcgccatc atccgtatac
      181 gaagaaaacg gcccaccata tggttgccat ccttcacgaa ttttttgcgc aagtgcatca
      241 gcaaggtctg acggcgacgc cgccctgacc acatcgtagt gtttaaatgt cataacctct
      301 cctgccgcca gcaaagtaat tatccggaat acacaatcat gtaacacata aaaaacacgc
      361 tataaaaaaa ccatgtctct tatataacaa aaaaacgccg acaatttaaa atgtcctctt
      421 attttaaaca aagaataaac gacaccagaa aaacaactca ggctgacaga ttgctttcaa
      481 aacatcactt ataatttatg gcatcattta aaaccttaag aaaataagga aagcaaatga
      541 actggatagt gattgatacg gtaatccagc cagcatgtga catatcattt tcagccatat
      601 ggtgtaacat aaaattaata ttatggtatc agtctgatat cttcctccct ccgggctgta
      661 tttttacacc aacccggaca ggcgtaatac tcaataataa agagctcccc gtcaccattt
      721 acaacgtgac cccatttaat aaaaacgtct ggaatttaat taaaagcagc caggaatgcc
      781 ctgcaaacac aggaaatatc acagataagt gttttaatca tcgctgcatt ctggaaatat
      841 gcccatacgg gcgaaaataa cagaatcatt cagataaaaa gcccctccgg agaggggctg
      901 aagccgcatt tctgtatcac catgaacatg gtgccgggtg cctcccggtg agttcagtcc
      961 ggtgtccctg aacccgcgta tctcgatcca ggttgtcgtc agagatgaca ccttatacac
     1021 cagtcgcccc tccgcacagg gggattcacc atgcgaaatt tttttaacaa atgctcagtc
     1081 tgacaggcaa ctgtcaactg actgaattgt gacacagatt acacttgtta cccacatacc
     1141 acgaatcagg ttatgcctca gtcattatta aactgcactt cagcaaatcc ggagcctgat
     1201 tcacaggtac tggatttgat tgtgacagtc attcctgtca actgagcact ttgcagtaac
     1261 ggttgcagat tccagcgact ggtccagtat tctttcccgt caaccttcac tgtaaatgtg
     1321 tcatcctcat tatacttgga aaactcaatt ttacctttag cacaatccgc cgccattgca
     1381 ttaacagaaa ctaatgcaaa taaaaccgcc ataaacatct tcttcatact taactccttt
     1441 atttacccgt tgtatataaa aactgtgact ttctgttcag aaacgctgca gctgtattac
     1501 tttcccataa tgtattgttt atttttataa cgggcctgtc gccagttatc tgacattctg
     1561 gttgactctc ttcattcacg gcgcgaacag aacgcgcccc ctgatgatgg caattcagta
     1621 taacggccac agtacccagt atcgctgata tattattaaa ggatattctc cccactctga
     1681 caccatcctc tccccgatac tccggaagca cattgctgat tcgcccccag ttcagagtga
     1741 ggtccacgtc tcccggcgtc atcgtataca caggagcagt ttcagacagt gcctgacgaa
     1801 attctctctg tatctgcctg aagcgtaagg cttctgccgt gacagtgaca aaacgcagaa
     1861 ctgctctgga tgcatctctg gtcattgtat taccactgaa ctccattaac gccagatatg
     1921 atgaaaccag tgagtgacga ctgatttgca ttccggaacg ttccagcgct gcgacacgtt
     1981 gcagagtggt ataactgctg tccgttgtca tggaaaccgt tgtcacatcg ggcactgata
     2041 tatgtgtaaa atctgaaaaa cggtagaaag tatttgttgc cgtattaacg aacccggcca
     2101 catataaatt attttgctca ataatcagac gaagatggtc aaaacgcgcc tgatagacat
     2161 caagccctcg tatatccaca gcaaaataac tgcccggcgg ggtgtggtta ataacagaca
     2221 ccgatgtggt cccctgagat atatgttcaa gaggggtcga tatctctgtc cgtatactat
     2281 ttaacgaaga gacataactt tgttgagtcg aaaagtctat cgtaaattcc cgggaatagg
     2341 ataccgaaga aaaacccagt aacaggcaca gtacccattt aaataatata cacttcatat
     2401 acaggtgttc cttttggctg aagtaatcag caccagaccc ggcgcagata taaaaaaggc
     2461 ccgcaaaagc gagcctggta aataaatatg gcgcgttgta ctggattcga accagtgacc
     2521 gattgcttag aaggcaattg ctctgtccgg ctgagctaac aacgctgaat accgataatg
     2581 gaccgccatc gaggactcga accccgcgca gccagcttcg aaggctggcg ctctgtcccg
     2641 atgagctaat ggcggtatgt gatatggtgg cccttgctgg atttgaacca gcgacctggc
     2701 gattatgagt cgctcgctct caccactgag ctaaagggcc gggcgcagga taataacggt
     2761 acgtaactaa tcctgcaata tcatccgttc tgactggcta aatcctgaac ttccctgacc
     2821 gtctgctcaa aacgttcagt ctccagctca acgccagttg cacgacgccc cagcgccagt
     2881 gcagctttga ctgtcgaacc cgaccccatg aaaaaatctg caaccaggtc acccggacga
     2941 ctgctcgcgc tgattatctg ctgcagcatt tctgccggtt tttcgcacgg atgtttcccg
     3001 ggatagtact gcaccggttt atgcgtccac acatccgtgt acggcacctg cgccgtcacg
     3061 ccaaaatacc gccgcagatg cttatattca ctctgcagtt ccgcatactg ccggttcagt
     3121 gaagtatacg tctccagcag ctggtggtgg ggcttttcca gttcaccgcg ctgatgcttc
     3181 tcttctgcca cccgggcaaa cagcgactgt aatttcagat aatcgctttc gttcggtagc
     3241 tgccactgac tggcactgaa ccagtgcgac accatgtttt tctttcctgt ggcatccact
     3301 atctgttttg ccgttatccc cagggcagca cgcgcatcac gaaagtaagc aatcagcgga
     3361 gccatcacat gctgtttcag tgccctgccc ttcgcctcat acccggcatc tttcggacga
     3421 tacggcccct gataatgttc cgcgaacaga atgcgctctg tggcggggaa atacgccctc
     3481 aggctttcct tgttgcaccc gttccagcgt ccggacggct tcgcccagat aatatggttc
     3541 agcacactga agcgttcacg catcatgatt tcaatgtcag atgccaggcg atggccacag
     3601 aacaggtaaa gacttccggc aggtttcagc acccgccaga actgcgccag acactggtcc
     3661 agccatttca ggtaatcctc atcacccgtc cactggttat cccagccctc aggcttcact
     3721 ttaaagtacg gcgggtccgt gactatcagg tcaacagaat tttcgggtaa cgaccggata
     3781 aattccaggc agtcggcgtt gattaactca caactggata tttttacagt attaaacatg
     3841 gatcattaag cctgtctctg ataggctcat accgcttttg cgcaaagcag atgggcctga
     3901 ggtttgcttg tgaccccaac gcatgagcag atggctggtg agtgccccta acacccacca
     3961 gccgcccatt taccacaaat aaaaaagcct tcaggactga aggcgtctgt aacaaccaaa
     4021 ctgatagtct gccagacccg ccataacaag ctgggtcagt attagctggc agcgttcgcg
     4081 tgaaaggtac gtattctgtg caatctcccc gactgtcgcc ggtgcggtgg cacttaattc
     4141 attaaacacc actctggcgg tttctgtcat atcctgctgt ttcagcatgt ctttttccct
     4201 tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagacc
     4261 ggtattcacc gccaccagcg cgtttaacgt actggtccga tttcaggcat aaaaaacccg
     4321 cctgacggcg gatttaagct gtgtggcaaa gtaaccactc ttaacatact gacatacttt
     4381 ttgcggaccg cactaatcat tttttacttt tttagcagcc agtcgtccat ctccagtctt
     4441 acccccagca cagacaaaca tccgtcaata aacccttcgg ctatctgcat ctcaattcgt
     4501 attgcttttt cgcttttctt tctcgtcctg gctatctgtc tttttgatat tcgcaacaaa
     4561 taatgagcaa tgagaagcga atactcctca ggttttttct gcttcagacg tgcaagacag
     4621 ttttcaatga taagtccgtc atcatcgcag caggctggac gtggtttagt ggtagatggt
     4681 aaaaggcctt tgaatccggc agcgatcgga gaatagtcca ccccggtgtt accacttgcc
     4741 gcccatgccc cccagcgttc aagaaccatc tgaatatcac gcatcaactt tctccacaaa
     4801 aatcaggaca gcacaccaat cgccagtgcg cgatcgataa aacgaaatat cagctccagt
     4861 tgggaaccat acttctcttc aaatgccacg gtatccgcat gcagttcgtc atggtgtttt
     4921 ctgcacaaag gcaacacaaa aaggtcatgc gcttttgtac ccattccacc ctgaccatgg
     4981 ccaatcaggt gatgtggatc gtcggctggc ttaccacaac acgcgcaagg ctgtgtctta
     5041 acccagcgcg tgtacttttc attaacccag cgacgacgtt tggggcgtaa cataaaagac
     5101 tccggcgact ccggatccac tttcagcgcc agcacctttt tcgctttatc ctggataatg
     5161 ctggtggcag gaaccgaagg cacaaggtca ctctcccggg tgacagacgg caaaacaggc
     5221 ttcggtaatc tcagggcctt acgggctgca ctttccggta aggcatccgc caggtcatta
     5281 cgaaccagcc accagcacag ttcaggcatt gtcacaacgt gactgtcatc aaaaccgaga
     5341 tcacgacgga ctacggacaa cacccagcgg gcacagttat ccgttgccat tgactccaga
     5401 cgttccgtga actgatcgcg aagcaggtta tcgcagtgcc agcacagacg gattgcgccc
     5461 ggcgcgtgtc gcattgtggt catgttctcg ctgtgccatc cggaatgagg ccactggcag
     5521 cctttttcac gaagtaacca gctctcaagg cattccacgc caccagcacg acggatcacc
     5581 gcctcatggc ggaacacggc ccgaacggca ggatcatccg ccagcggttg tgatgctgcc
     5641 ggaacggcac cactggcaaa agatgaataa cgttccggct caggctccag caggacacgc
     5701 ccctgcataa acaggggcat cagctctgaa ccgggtctga acaagacgat ccccatacgc
     5761 ggggcaattt caggggtcag cagtgctctc acggtcacct cagcgaacgg tattgcatga
     5821 acgcaggaga aaaaattcag ccatcacgca gtaaactctt tcaccagcgt ttcaaactgg
     5881 cttacctggc cttccagttc cgccacgcaa tccaccagct catccaccgc cttttgtgtg
     5941 cgatgttttg cctgcagcag atcacgaagc gccggagtaa gctgcttgcg gagcgtatct
     6001 tttgccacgc tcgttttttc catctgttca gcacaacgaa gcatctcctg cgcctgccga
     6061 cgaagttgtt ccggtgaaac agtggtggtt ctgttgttca aaataaacgc tccgttttac
     6121 tacccgacat gcggttattg ctgtatctgc gcggattgcc cggcgtcatg ggagtggaaa
     6181 gaacccgggc actctcctgg tccacaggca gaaaatgccc gttatgaaaa cgccggtaaa
     6241 tggtacccag cgtgccatta cgctgtttcg tgatgttgat ttcagctatg cctctcgcct
     6301 gtgtctccgg gttgtacacc tcatccctgt aaagcatcag aatgatgtct gcatccgcct
     6361 ctatttcccc agagtttttc aggtccgagt tcatggggcg tttattgggt ctggattcca
     6421 cgccacggga gagctggctc agagcaatca gcggaaaacc gccggatttt gccaggcttt
     6481 taagtccctt tgagatttcc cccacagcaa ggtcgtgacg ccccgtgctg cgggttttaa
     6541 tcaggccgag gtaatcgacc accaccagcg ccgtttccgg gtgtttcatc cggtggtgcc
     6601 tcgtggttgc acatatctca tcaatggtca gatttgcctg gtccaccatc cagatattac
     6661 gccccgtcat tcgtcccacg ccctgtgaga aacgcgccca gtcttcatct tcaaaacggg
     6721 caacagactt aagacgggat accggcattc cccctgctgc agacaccata cgttcaccaa
     6781 tctggatgtt cgccatctcc atggtgaaca gaagcacgcc atgcccctgc tcagtcacct
     6841 tgtcgatgat gtccagcgca agttctgttt tccccatcga aggacgggcg gcaatgaata
     6901 ccaggtctcc gggctccata ccgcccgttt ttgcgtccag ttcatcaata tcggtcatca
     6961 gcgccctgga tttctccagt ccctgattcc ggcattcaac acggcccacc acttccggaa
     7021 ggacatcatc aatgtgaacc ggctgaatga cgccctttcc ggtcgacagt gaggccatca
     7081 tgttctgcgc atccttcagg gcatcttctg ccgcttcgca ggtatgtgca tcacgtaaat
     7141 tctgtaatgc ttcagtcagt gttttttctg catcgcgcag tgcggcattg cgtcgcagcg
     7201 ctgcaacata gtgctccagt gaagacttca cccaggtttt gcgcccggtg tcggtaatca
     7261 ccggggcaag ttccggcatc tcattgcaca gcagcacggg gtcaatgacg ccggatacgc
     7321 gggcctgtct gcaaatcccc gcgtaaatat cccggtactg acgcacgaaa aatacatccg
     7381 ccggaagcgt ggccagaata tccatcactt ccggatcagc cccacgcaga aaaaacgcgc
     7441 caatcaccgc accttccagg tcatcattac gccacaccgg atttgttggg tttgtcatgc
     7501 tgccacacct ctgatatgcg cacggtagct ttcccaacca aacaccaggc agttacgccc
     7561 accatcagta acgcgatcca caatccgttc accaatggat tccttaagct gttcaaaagt
     7621 caggttgctg atcaaaattg tcggtaaaac gctttcgtaa cgcgcattta tgatttcctg
     7681 caggatggtt atctccgcag gcgtaccgaa ctgcacacca acctcatcga taataagcag
     7741 gtccagcgat acaaaatggt caataacttc atcttcagtg cgctcagaat tgtgacgcca
     7801 ggtatttttc acagcgcggg tcagtcgcat aacatcagta atttcgacgg tcgccaggtg
     7861 gtggcgaata atattttttg ccattgatac agccaggtga tttttcccgg taccgcaatt
     7921 gccaatcatg atcatgctgg tgcctgcagc gagacatttt ttccaggaag cggcgtagcg
     7981 ctgacaggct tcaaggtttt tctgtgcgtc agtattcacc gcctgataat tatcaaactc
     8041 acaatcctgg aaccggcggg caatagcggc cagatcgagt aattcacgaa ccttcagggc
     8101 gcgtaattcg tcatacacgc agtccagctc ctcactgagg caggaaaggc aaccggacac
     8161 ccgtttgaca gctttccctc ttacatccgg gcctgtcagc acatagcgtg tgtattttcc
     8221 gtgtttcccg caggacaccg tctcagtgct ttgcacccaa tgctcacagc gccacggacg
     8281 ctttccgcca cggacaaatg ccagctcctc ttccaggtct gccttgcggt taagtaactg
     8341 cgctttgtcg tgttgcatgt attgtttgtt caggaaatta gtcattttca caccccacaa
     8401 aatcaccagt tgaagttcgt tgagccgtag tcctgttcac tgaatcccga gatcgggagg
     8461 cttttgcccc gcccacctcc gggaactgct gactgttgcc aggattcgtc aaagtgccga
     8521 tcggggccaa agaacgtcga tgcctgcttc acgaactggg tgccggtatt tcctgagaca
     8581 cgcacccagg cggcatagcg tttcacaccg ttgagcatgg tttcgggtgt cacaccttcc
     8641 ctgatacggg ctttccaggc tttgaaggct gctgacttgg aattaccacc agcacgtttg
     8701 ggatattcct gccaggcctg ttcaaattcc ggtgaatatt cctgtcgggc agaacgtgct
     8761 ggtgcagacg cgtcagcgga tgcgccaata gtgtttttac tctctgtagt attctctgaa
     8821 gtaatctctg ttgtattctc tgtaagatcg aaattggttt tcccttctcc gcggcgaggg
     8881 gtttcccgtg tccgcggtga aggctttccc tcctccgcga aatcgggttt tacagtttcc
     8941 cgaaaacggg tttccccatt tcgggaaaag tgattgtttt cattgataat ttcattaaga
     9001 cgctcacaat ctatacggta gaacattttg tgctcaagac gcttgttggt ttcaaccaaa
     9061 atgcctctgg acacaagatg cttacgcgct acagcctgtt gttcaaatgt aagtccggtt
     9121 tcgtgttgta tctcttcacg cgttttatgt acgccttccg ctgcatgtgc tttatcctgc
     9181 cagtagaaaa tctgaccaaa gaaaataaca gcgtgcggac ttcccatgta tttaacgagc
     9241 ccagggtaat aagcaaccgg atgcccaaaa tcgagcagaa gatcagacgg acgcatagcc
     9301 acctcccagg cgcttaaaca ttttcccgga ctgaaaagcc accagcggat aactcagggt
     9361 atgagtacgt ccctgaacct ggcagacaac cttctggctt tctgtattga ccaggcaaac
     9421 ccgcagaacg tgaccgttgc tggtggcgaa ccactgcccc acacgggggc aacggttgta
     9481 tcggtgatac agggaattaa cgatgtagcg gatcatggac gcacctccgc cgtagttacg
     9541 tatttaaccg ggctaccttt cattgagatg gtttcacaca tctctgccgc tttcagttcc
     9601 gccgtttttc tggatttata gcgacggtgc cagacagata catccgtgcg aactgataca
     9661 tcgtttctgt attccgtagt ggagatgatg atttcgtaac taatcatggg cgtacctccc
     9721 attgattacg gcgaaaagca gtgtgattca ggctggtttc agcctcatgg aatgctccaa
     9781 tgcagctctc gtagtaccgc attgtgcgta gacttaaccc aagctgaagc atcatcaggc
     9841 catcaagggt gatgtaataa ccacgcaggg agtcaccata gatgtggtaa gtacccggta
     9901 tgaagttgcg ggtaaaaaat tcgcgcgagc agttcagata ctcgattttg tcgacgatgt
     9961 tctggtgcat gcgcttaaag tggcaggcaa catgcaggga gaaaataaca gctttgccgt
    10021 tgacgacttc gattttgagg aatggggaag ttgggactgt agccatgatg gcagcctcct
    10081 tgatcggtga aaaacttcca ccaccggagc tgcgaaactc atgggtggtg aactgaacag
    10141 ggttcgcagt accggcgatc aaggaaaccg gcgagccttt cggctcccct gcccagccca
    10201 ccataattct ggcgtgcgtg agcgcagacg ataaaaaaga cgctggcgcg tcgtatatcg
    10261 ccttgatcaa ttccgggctg cgacccccgg cacccgcttt ataaggtgca gagacagtgt
    10321 aacgtcccga aattgcagaa tcaatatttg gtcttgaaat gatcatatag ctgctgatat
    10381 ctttagaact gttcttggat gtttcggagc cgttttatgc gaaacagctc cccgttattg
    10441 atgttgagtg agccgggtta ctcccggctt tttttcaccg ctgccaacca ataacctgaa
    10501 ataaccccat tttcgggtga taccagcgag tccctcgcgg ttctgcttcc tccataaccc
    10561 gataaaaagc agccataaaa ggttccacgg caacaattgc gcgacgtgac aacaatccgt
    10621 ccggcgtcat gaactcatgg gtgtctgtag gaatttgata atcgttcacc agattgcggc
    10681 atttcgcatc tgacatacct gttttcgcca ccagctgacg gtatcctgca taaccatcgc
    10741 gtatggtgcc tcttttgatt tgttcgacag tttcggcaac gtggctaact ttttcttcca
    10801 tcttgtcgag gcgtttttgc tgacgaactg cttcaagagc catcgcggca accatttcga
    10861 tttcgctcat tggtttgcgc acctgctctt ccagttcgcg ccaacgatct accaggcgag
    10921 cagtgaattc aggacagagc tgtgcgacga caatgatact gtcgcgctta ccttgttctc
    10981 cttcaaacag gtaatgctca tattgaactt taaaacctaa gttattgatt ttctcggaaa
    11041 cagccattgg cggtttccgg atgatgtttt tagcaaccag gcgttcgata ctacgtttaa
    11101 catctgagtg ctgactaccc accagctctg cgatctcaac gctggtcatg gatgctttat
    11161 cgttaaaaat tgcggtgttc actgtttatc tccttcgcac actccatctt ctgtgtgcgc
    11221 taagcttggg tgtgggaaaa gttttggctt atctggccta agctcatgag cgggaatccc
    11281 tgttacggca gaaacgtctg gaacatgctc cacccctaca agtccaatct ttctccatcg
    11341 ggaaacagat ggctgtttga ccccaacggc gcgagctaat gcatttacgc cccctgcaat
    11401 atctattgct ttctcaatcg ctgatttcat aaaacacacc taacaattgc tttttctatc
    11461 aaaaaataat agcaacacct attccaccat gcaatagaca cacttataga acgcatctac
    11521 aatgtaatag cggaggctat atttatgtcg aaatcacaaa tgagcatgtt gagaaccctt
    11581 gcagataggc tcaactttgc aatgtacgaa atgggaatga gccaagctca gttggctaag
    11641 gcagcaaaca tggctcaacc gaccatttgg cgaatagcat cggggaatgc aagaggaaca
    11701 acgaaaattg tcgatctcgc taatgctctc ggtgttacac cggaatggtt aagttctggt
    11761 gtaggttcta tgagggcaga gaacaagaaa ccatctattc caccaaaatc cgaatggggg
    11821 aagatagagt cgtgggatga gcacacgcct ctaagtgatg atgaggttga agtccctttt
    11881 cttaaagata ttgagtttgc gtgtggtact gggaaagtga ttagcgaaga tcataatgga
    11941 ttaaaactta ggttttcaaa agctaccctt cgtcggatcg gcgcaaactc tgacggaagt
    12001 ggagtgctat gttttccggc gactggtaac agtatggagc ctataatccc tgatggaacg
    12061 accgtagcta ttgacaccaa caacaaaaga attgtcgatg gtaagttgta tgctattgga
    12121 caagatgatg gttgtggcgg acaactaaag cgcattaaac aactacacag aagaccaggt
    12181 ggaaaattga ttatccgcag ttacaacagt gacgaatacc cagatgaaga aaccagtatt
    12241 gataaggttg atattatcgg gcgtttattc
//
LOCUS       DAZUGK010000133        12208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZUGK010000133 DAZUGK010000000
VERSION     DAZUGK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.134"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(137..1420)
                     /gene="gltA"
                     /locus_tag="ACHY5W_003263"
     CDS             complement(137..1420)
                     /gene="gltA"
                     /locus_tag="ACHY5W_003263"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFS4448119.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1626..1733)
                     /locus_tag="ACHY5W_003264"
     CDS             complement(1626..1733)
                     /locus_tag="ACHY5W_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448120.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2114..2518
                     /gene="sdhC"
                     /locus_tag="ACHY5W_003265"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2114..2518
                     /gene="sdhC"
                     /locus_tag="ACHY5W_003265"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS4448121.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2512..2859
                     /gene="sdhD"
                     /locus_tag="ACHY5W_003266"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2512..2859
                     /gene="sdhD"
                     /locus_tag="ACHY5W_003266"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFS4448122.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VDVIYGFVVVWGV"
     gene            2859..4625
                     /gene="sdhA"
                     /locus_tag="ACHY5W_003267"
     CDS             2859..4625
                     /gene="sdhA"
                     /locus_tag="ACHY5W_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFS4448123.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4641..5357
                     /gene="sdhB"
                     /locus_tag="ACHY5W_003268"
     CDS             4641..5357
                     /gene="sdhB"
                     /locus_tag="ACHY5W_003268"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFS4448124.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNEQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5658..8459
                     /gene="sucA"
                     /locus_tag="ACHY5W_003269"
     CDS             5658..8459
                     /gene="sucA"
                     /locus_tag="ACHY5W_003269"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFS4448125.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKTQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8474..9691
                     /gene="odhB"
                     /locus_tag="ACHY5W_003270"
     CDS             8474..9691
                     /gene="odhB"
                     /locus_tag="ACHY5W_003270"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFS4448126.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALTPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9785..10951
                     /gene="sucC"
                     /locus_tag="ACHY5W_003271"
     CDS             9785..10951
                     /gene="sucC"
                     /locus_tag="ACHY5W_003271"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFS4448127.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10951..11820
                     /gene="sucD"
                     /locus_tag="ACHY5W_003272"
     CDS             10951..11820
                     /gene="sucD"
                     /locus_tag="ACHY5W_003272"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFS4448128.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2944 a         3118 c         3335 g         2811 t
ORIGIN      
        1 gatatgtatt cttgtaacgt gtaattatgt atttgtgtcc gattacgttt ttttgtcgat
       61 agacataact aacgaacaaa aaaaagcccg ccagatattc aggcgggcta aacgatatat
      121 ggttcaaatc agataattaa tgtttaacat ctgatttaaa gtcgcgtttt tcatatcctg
      181 tatacagctg acgcggacgg gcaatcttca taccgtcact gtgcatttcg ctccagtggg
      241 cgatccagcc aacggtacgt gccattgcga aaatgacggt gaacatggaa gacggaatac
      301 ccatcgcttt caggatgata ccagagtaga aatcgacgtt cgggtacagt ttcttctcga
      361 taaagtacgg gtcgttcagc gcgatgtttt ccagctccat agccacttcc agcaggtcat
      421 ctttggtgcc cagctctttc agaacttcat ggcaggtttc acgcattacg gtggcgcgcg
      481 ggtcgtaatt tttgtacacg cggtgaccga agcccatcag gcggaaagaa tcatttttat
      541 ctttcgcacg acgaacaaat tccggaatgt gtttaacgga gctgatttct tccagcattt
      601 tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca cagtgaagca atacctgctg
      661 cgatacaggc aaacgggttc gcacccgaag agccagcggt acgcacggtg gaggtagagg
      721 cgttctgttc atggtcagcg tgcaggatca gaatacggtc catagcacgt tccagaatcg
      781 gattaacttc atacggttcg cacggcgtgg agaacatcat attcaggaag ttaccggcat
      841 aggagagatc gttgcgcgga taaacaaatg gctgaccaat ggaatacttg taacacatcg
      901 ctgccatggt cggcattttc gacagcaggc ggaacgcggc aatttcacgg tgacgaggat
      961 tgttaacatc cagcgagtcg tgatagaacg ccgccagcgc gccggtaata ccacacatga
     1021 ctgccattgg atgtgagtcg cgacggaaag cgtggaacag acgggtaatc tgctcgtgga
     1081 tcatggtatg acgggtcacc gtagttttaa attcgtcata ctgttcctga gtcggttttt
     1141 caccattcag caggatgtaa caaacttcca ggtagttaga atcggtcgcc agctgatcga
     1201 tcgggaaacc gcggtgcagc aaaatacctt catcaccatc aataaaagta attttagatt
     1261 cgcaggatgc ggttgaagtg aagccagggt caaaggtgaa cacacctttt gaaccgagag
     1321 tacggatatc aataacatct tgacccagcg tgcctttcag cacatccagt tcaacagctg
     1381 tgtccccgtt gagggtgagt tttgcttttg tatcagccat ttaaggtctc cttagcgcct
     1441 tattgcgtaa gactgccgga acttaaattt gccttcgcac atcaacctgg ctttacccgt
     1501 ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc tgggtacaga gctctgggcg
     1561 cttgcaggta aaggatccat tgatgacgaa taaatggcga atcaagtact tagcaatccg
     1621 aattattaaa cttgtctacc actaataact gtcccgaatg aattggtcaa tacttccaca
     1681 ctgttacata agttaatctt aggtgaaata ccgacttcat aacttttacg cattatatgc
     1741 ttttcctggt aatgtttgta acaactttgt tgaatgattg tcaaattaga tgattaaaaa
     1801 ttaaataaat gttgttatcg tgacctggat cactgttcag gataaaaccc gacaaactat
     1861 atgtaggtta attgtaatga ttttgtgaac ggcctatact gccgccaggt ctccggaaca
     1921 ccctgcaatc ccgagccacc cagcgttgta acgtgtcgtt ttcgcatctg gaagcagtgt
     1981 tttgcatgac gcgcagttat agaaaggacg ctgtctgacc cgcaagcaga ccggaggaag
     2041 gaaatcccga cgtctccagg taacagaaag ttaacctctg tgcccgtagt ccccagggaa
     2101 taataagaac agcatgtggg cgttattcat gataagaaat gtgaaaaaac aaagacctgt
     2161 taatctggac ctacagacca tccggttccc catcacggcg atagcgtcca ttctccatcg
     2221 cgtttccggt gtgatcacct ttgttgcagt gggtatcctg ctgtggcttc tgggtaccag
     2281 cctctcttcc cctgaaggtt tcgagcaagc ttccgcgatt atgggcagct tcttcgtcaa
     2341 atttatcatg tggggcatcc ttaccgctct ggcgtatcac gtcgtcgtag gtattcgcca
     2401 catgatgatg gattttggct atctggaaga aacattcgaa gcgggaaaac gctccgccaa
     2461 aatctccttt gttattactg tcgtgctttc acttctcgca ggagtcctcg tatggtaagc
     2521 aacgcctccg cattaggacg caatggcgta catgatttca tcctcgttcg cgctaccgct
     2581 atcgtcctga cgctctacat catttatatg gtcggttttt tcgccaccag tggcgagctg
     2641 acatatgaag tctggatcgg tttcttcgcc tctgcgttca ccaaagtgtt caccctgctg
     2701 gcgctgtttt ctatcttgat ccatgcctgg atcggcatgt ggcaggtgtt gaccgactac
     2761 gttaaaccgc tggccttgcg cctgatgctg caactggtga ttgtcgttgc actggtggtt
     2821 gacgtgattt atggattcgt tgtggtgtgg ggtgtgtgat gaaattgcca gtcagagaat
     2881 ttgatgcagt tgtgattggt gccggcggcg caggtatgcg cgcggcgctg caaatttccc
     2941 agagcggcca gacctgtgcg ctgctctcta aagtcttccc gacccgttcc cataccgttt
     3001 ctgcgcaagg cggcattacc gttgcgctgg gtaataccca tgaagataac tgggaatggc
     3061 atatgtacga caccgtgaaa gggtcggact atatcggtga ccaggacgcg attgaatata
     3121 tgtgtaaaac cgggccggaa gcgattctgg aactcgaaca catgggcctg ccgttctcgc
     3181 gtctcgatga tggtcgtatc tatcaacgtc cgtttggcgg tcagtcgaaa aacttcggcg
     3241 gcgagcaggc ggcacgcact gcggcagcgg ctgaccgtac cggtcacgca ctgttgcaca
     3301 cgctttatca gcagaacctg aaaaaccaca ccaccatttt ctccgagtgg tatgcgctgg
     3361 atctggtgaa aaaccaggat ggcgcggtgg tgggttgtac cgcactgtgc atcgaaaccg
     3421 gtgaagtggt ttattttaaa gcccgcgcta ccgtgctggc gactggcgga gcagggcgta
     3481 tttatcagtc taccactaac gcccacatta acaccggcga cggcgtcggc atggctatcc
     3541 gcgcaggcgt accggtgcag gatatggaaa tgtggcaatt ccacccgacc ggcattgccg
     3601 gtgcgggcgt actggtcacc gaaggttgcc gtggtgaagg cggttatctg ctgaacaaac
     3661 atggcgaacg ctttatggag cgttatgcgc cgaacgccaa agacctggcg ggccgtgatg
     3721 tggtggcacg ttccatcatg atcgaaatcc gtgaaggtcg cggctgtgat ggtccgtggg
     3781 ggccacacgc gaaactgaaa ctcgatcacc tgggtaaaga agttctcgaa tcccgtctgc
     3841 cgggtatcct ggagctttcc cgtaccttcg ctcacgttga tccggtgaaa gagccgattc
     3901 cggttatccc aacctgtcac tacatgatgg gcggtattcc gaccaaagtt accggtcagg
     3961 cgctgactgt gaatgagaaa ggcgaagatg tggttgttcc ggggctgttc gcggtaggtg
     4021 aaattgcctg cgtatcggta cacggtgcta accgtctggg cggcaactcg ctgctggacc
     4081 tggtggtctt tggtcgcgca gcgggtctgc atctgcaaga gtctatcgcc gagcagggcg
     4141 cattgcgcga tgccagcgag tctgatgttg aagcatctct ggatcgtctg aaccgctgga
     4201 acaataatcg taacggtgaa gatccggtgg cgatccgtaa agcactgcaa gaatgtatgc
     4261 agcataactt ctcggtcttc cgtgaaggtg atgcgatggc gaaaggtctt gagcagttga
     4321 aagttatccg cgagcgtctg aaaaatgccc gtctggatga cacttccagc gagttcaaca
     4381 cccagcgcgt tgagtgcctg gaactggata acctgatgga aacggcgtat gcaacggctg
     4441 tttctgccaa cttccgtacc gaaagccgtg gcgcgcatag ccgcttcgac ttcccggatc
     4501 gtgatgatga aaactggctg tgccactccc tgtatctgcc agagtcggaa tccatgacgc
     4561 gccgaagcgt caacatggaa ccgaaactgc gcccggcatt cccgccgaag attcgtactt
     4621 actaatgcgg agacaggaaa atgagactcg agttttcaat ttatcgctat aacccggatg
     4681 ttgatgatgc tccgcgtatg caggattaca ccctggaagc ggatgaaggt cgcgacatga
     4741 tgctgctgga tgcgcttatc cagctgaaag agaaagatcc cagcctgtcg ttccgtcgct
     4801 cctgtcgtga aggtgtgtgc ggttccgacg gtctgaacat gaacggcaag aatggtctgg
     4861 cctgtattac cccgatttcg gcactcaacc agccgggcaa gaagattgtg attcgccctc
     4921 tgccaggttt accggtgatc cgcgatttgg tggtagacat gggacaattc tatgcgcaat
     4981 atgagaaaat taagccttac ctgttgaata atgaacaaaa tccgccagct cgcgagcatt
     5041 tacagatgcc agagcagcgc gaaaaactcg acgggctgta tgaatgtatt ctctgcgcat
     5101 gttgttcaac ctcttgtccg tctttctggt ggaatcccga taagtttatc ggcccggcag
     5161 gcttgttagc ggcatatcgt ttcctgatcg atagccgtga taccgagact gacagccgcc
     5221 tcgacggttt gagcgatgca ttcagtgtat tccgctgtca cagcatcatg aactgcgtca
     5281 gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg ccatatcaag tcgatgttgt
     5341 tgcaacgtaa tgcgtaaacc gtaggcctga taagacgcgc aagcgtcgca tcaggcaacc
     5401 agtgccggat gcggcgtgaa cgccttatcc ggcctacaag tcattacccg taggcctgat
     5461 aagcgcagcg catcaggcgt aacaaagaaa tgcaggaaat ctttaaaaac tgcccctgac
     5521 actaagacag tttttaaagg ttccttcgcg agccactacg tagacaagag ctcgcaagtg
     5581 aaccccggca cgcacatcac tgtgcgtggt agtatccacg gcgaagtaag cataaaaaag
     5641 atgcttaagg gatcacgatg cagaacagcg ctttgaaagc ctggttggac tcttcttacc
     5701 tctctggcgc aaaccagagc tggatagaac agctctatga agacttctta accgatcctg
     5761 actcggttga cgctaactgg cgttcgacgt tccagcagtt acctggtacg ggagtcaaac
     5821 cggatcaatt ccactctcaa acgcgtgaat atttccgccg cctggcgaaa gacgcttcac
     5881 gttactcttc aacgatctcc gaccctgaca ccaatgtgaa gcaggttaaa gtcctgcagc
     5941 tcattaacgc ataccgcttc cgtggtcacc agcatgcgaa tctcgatccg ctgggactgt
     6001 ggcagcaaga taaagtggcc gatctggatc cgtctttcca cgatctgacc gaagcagact
     6061 tccaggagac cttcaacgtc ggttcatttg ccagcggcaa agaaaccatg aaactcggcg
     6121 aactgctgga agccctcaag caaacctact gcggcccgat tggtgccgag tatatgcaca
     6181 ttaccagcac cgaagaaaaa cgctggatcc aacagcgtat cgagtctggt cgcgcgactt
     6241 tcaatagcga agagaaaaaa cgcttcctaa gcgaactgac cgccgctgaa ggccttgaac
     6301 gttacctcgg cgcaaaattc cctggcgcaa aacgcttctc gctggaaggc ggtgacgcgt
     6361 taatcccgat gcttaaagag atgattcgcc acgctggcaa cagcggcacc cgcgaagtgg
     6421 ttctcgggat ggcgcaccgt ggtcgtctga acgtgctggt gaacgtgctg ggtaaaaaaa
     6481 cgcaagactt gttcgacgag tttgccggta aacataaaga acacctcggc acgggtgacg
     6541 tgaaatacca catgggcttc tcgtctgact tccagaccga tggcggcctg gtgcacctgg
     6601 cgctggcgtt taacccgtct caccttgaga ttgtaagccc ggtagttatc ggttctgttc
     6661 gtgcccgtct ggacagactt gatgagccga gcagcaacaa agtgctgcca atcaccatcc
     6721 acggtgacgc cgcagtgacc gggcagggcg tggttcagga aactctgaac atgtcgaaag
     6781 cgcgtggtta tgaagttggc ggtacggtac gtatcgttat caacaaccag gttggtttca
     6841 ccacctctaa tccgctggat gctcgttcta caccgtactg tactgatatc ggtaagatgg
     6901 ttcaggcacc gattttccac gttaacgcgg atgatccgga agccgttgcc tttgtgaccc
     6961 gtctggcgct cgatttccgt aacaccttta aacgtgatgt cttcatcgac ctggtgtgct
     7021 accgccgtca cggccacaac gaagccgacg agccgagcgc aacccagccg ctgatgtatc
     7081 agaaaatcaa aaaacatccg acgccgcgca aaatctatgc tgacaagctg gagcaggaaa
     7141 aagtggcgac gctggaagat gccaccgaga tggttaacct gtaccgcgat gcgctggatg
     7201 ctggcgattg cgtagtggca gagtggcgtc ctatgaacat gcactctttc acctggtcgc
     7261 cgtatctcaa ccacgaatgg gacgaagagt acccgaacaa agttgagatg aagcgcctgc
     7321 aggaactggc taaacgcatc agtacggtgc cggaagcggt tgaaatgcag tctcgcgttg
     7381 ccaaaattta tggcgatcgc caggcgatgg ctgccggtga gaaactgttc gactggggcg
     7441 gcgcggaaaa cctcgcttac gccacgctgg ttgatgaagg cattccggtt cgcctgtcgg
     7501 gtgaagactc cggtcgcggt accttcttcc accgccacgc ggtgatccac aaccagtcta
     7561 acggttccac ttacacgccg ctgcaacaca tccataacgg ccagggcgcg ttccgtgtct
     7621 gggactccgt actttctgaa gaagccgtgc tggcgtttga atacggttat gccaccgcag
     7681 aaccacgcac cctgaccatc tgggaagcgc agttcggtga cttcgccaac ggcgcacagg
     7741 tggttatcga ccagttcatc tcctctggcg agcagaaatg gggccggatg tgtggcctgg
     7801 tgatgttgct gccgcacggt tacgaagggc aggggccaga gcactcctcc gcgcgtctgg
     7861 aacgttatct gcaactttgt gctgagcaaa acatgcaggt gtgcgtaccg tctaccccgg
     7921 cacaggttta ccacatgctg cgtcgtcagg cgctgcgcgg gatgcgtcgt ccgctggtcg
     7981 tgatgtcgcc gaaatccctg ctgcgtcatc cgctggcggt ttccagcctc gaagaactgg
     8041 cgaacggcac cttcctgcca gccatcggtg aaatcgacga gcttgatccg aagggcgtga
     8101 agcgcgtagt gatgtgttct ggtaaggttt attacgacct gctggaacaa cgtcgtaaga
     8161 acaatcaaca cgatgtcgcc attgtgcgta tcgagcaact ctacccgttc ccgcataaag
     8221 cgatgcagga agtgttgcag cagtttgctc acgtcaagga ttttgtctgg tgccaggaag
     8281 agccgctcaa ccagggcgca tggtactgca gccagcatca tttccgtgaa gtgattccgt
     8341 ttggggcttc tctgcgttat gcaggccgcc cggcctccgc ctctccggcg gtagggtata
     8401 tgtccgttca ccagaaacag caacaagatc tggttaatga cgcgctgaac gtcgaataaa
     8461 taaaggatac acaatgagta gcgtagatat tctggtccct gacctgcctg aatccgtagc
     8521 cgatgccacc gtcgcaacct ggcataaaaa acccggcgac gcagtcgtac gtgatgaagt
     8581 gctggtagaa atcgaaactg acaaagtggt actggaagta ccggcatcag cagacggcat
     8641 tctggatgcg gttctggaag atgaaggtac aacggtaacg tctcgtcaga tccttggtcg
     8701 cctgcgtgaa ggcaacagcg ccggtaaaga aaccagcgcc aaatctgaag agaaagcgtc
     8761 cactccggcg caacgccagc aggcgtctct ggaagagcaa aacaacgatg cgttaacccc
     8821 ggcgatccgt cgcttgctgg ctgaacataa cctcgacgcc agcgccatta aaggcaccgg
     8881 cgtgggtggt cgtctgaccc gtgaagatgt ggaaaaacat ctggcgaaat ccccggcgaa
     8941 agagtctgca ccggcagcgg ctgctccggc ggcgcaaccg gctctggctg cacgtagtga
     9001 aaaacgtgtc ccgatgactc gcctgcgtaa gcgtgtggca gagcgtctgc tggaagcgaa
     9061 aaactccacc gccatgctga ccacgttcaa cgaagtcaac atgaagccga ttatggatct
     9121 gcgtaagcag tacggtgaag cgtttgaaaa acgccacggc atccgtctgg gctttatgtc
     9181 cttctacgtg aaagcggtgg ttgaagccct gaaacgttac ccggaagtga atgcttctat
     9241 cgacggcgat gacgtggttt accacaacta tttcgacgtc agcatggcgg tttctacgcc
     9301 gcgcggcctg gtgacgccgg ttctgcgtga tgtcgatacc ctcggcatgg cagacatcga
     9361 gaagaaaatc aaagagctgg cagtcaaagg ccgtgatggc aagctgacgg ttgaagatct
     9421 gaccggtggt aacttcacca tcaccaacgg tggtgtgttc ggttccctga tgtctacgcc
     9481 gatcatcaac ccgccgcaga gcgcaattct gggtatgcac gctatcaaag atcgtccgat
     9541 ggcggtgaat ggtcaggttg agatcctgcc gatgatgtac ctggcgctgt cctacgatca
     9601 ccgtctgatc gatggtcgcg aatccgtggg cttcctggta acaatcaaag agttgctgga
     9661 agatccgacg cgtctgctgc tggacgtgta gtagtttaag tttcacctgc actgtagacc
     9721 gacgtggata gggtgtcggt ctacggttta aaagataacg attactgaag gatggacaga
     9781 acacatgaac ttacatgaat atcaggcaaa acaactgttt gcccgctatg gcttaccagc
     9841 accggtgggt tatgcctgta ctactccgcg cgaagcagaa gaagccgctt caaaaatcgg
     9901 tgccggtccg tgggtagtga aatgtcaggt tcacgctggt ggccgcggta aagcgggcgg
     9961 tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt gcagaaaact ggctgggcaa
    10021 gcgtctggta acgtatcaaa cagatgccaa tggccaaccg gttaaccaga ttctggttga
    10081 agcggcgacc gatatcgcta aagagctgta tctcggtgcc gttgtagacc gtagttcacg
    10141 tcgcgtggtc tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag tggcggaaga
    10201 aactccgcac ctgatccata aagttgcgct tgatccgttg actggcccga tgccgtatca
    10261 gggacgcgag ctggcgttca aactgggtct ggaaggtaaa ctggttcagc agttcaccaa
    10321 aatcttcatg ggcctggcga ccattttcct ggagcgcgac ctggcgctga tcgaaatcaa
    10381 cccgctggtc atcaccaaac agggcgatct gatttgcctc gacggcaaac tgggcgctga
    10441 tggcaacgcc ctgttccgcc agcctgatct gcgcgaaatg cgtgaccagt cgcaggaaga
    10501 tccgcgtgaa gcacaggctg cacagtggga actgaactac gttgcgctgg acggtaacat
    10561 cggttgtatg gttaacggcg caggtctggc gatgggtacg atggacatcg ttaaactgca
    10621 cggcggcgaa ccggctaact tcctggacgt tggcggcggc gcaaccaaag aacgtgtaac
    10681 cgaagcgttc aaaatcattc tctctgacga caaagtgaaa gccgttctgg ttaacatctt
    10741 cggcggtatc gttcgttgcg acctgatcgc tgacggtatc atcggcgcgg tagcggaagt
    10801 gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac tcggcgcgaa
    10861 gaaactggct gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg atgcagctca
    10921 gcaagttgtt gccgcagtgg aggggaaata atgtccattt taattgataa aaacaccaag
    10981 gttatctgcc agggctttac cggtagccag gggactttcc actcagaaca ggctattgca
    11041 tacggcacta aaatggttgg cggcgtaacc ccaggtaaag gcggcaccac ccacctcggc
    11101 ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta ctggcgctac cgcttctgtt
    11161 atctacgtac cagcaccgtt ctgcaaagac tccattctgg aagccatcga tgcaggcatc
    11221 aaactgatta tcaccatcac tgaaggcatc ccgacgctgg atatgttgac cgtgaaagtg
    11281 aaactggacg aagcaggcgt tcgtatgatc ggcccgaact gcccaggcgt tatcaccccg
    11341 ggtgaatgca aaatcggtat ccagccgggt cacattcaca aaccgggtaa agtgggtatc
    11401 gtttcccgtt ccggtacact gacctatgaa gcggttaaac agaccacgga ttacggtttc
    11461 ggtcagtcga cctgtgtcgg tatcggcggt gacccgatcc ctggctctaa ctttatcgac
    11521 attctcgaaa tgttcgaaaa agatccgcag accgaagcga tcgtgatgat cggtgagatc
    11581 ggcggtagcg ctgaagaaga agcagctgcg tacatcaaag agcacgttac caagccagtt
    11641 gtgggttaca tcgctggtgt gactgcgccg aaaggcaaac gtatgggcca cgcgggtgcc
    11701 atcattgccg gtgggaaagg gactgcggat gagaaattcg ctgctctgga agccgcaggc
    11761 gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt tctgaaataa
    11821 atatctgtaa taagaaatag ccctcgccgc ttccctctac aggaatggcg aagggctgtc
    11881 ggtttcgaca tggttggcca tcgtatgatg gcctttttta tgccttaaac ggttaaccac
    11941 ggtcgaaatc tgactgcagg cttgactgca tgaccacagc cactgtggat tgccgcaacc
    12001 gaccgcaagc cacgaataag aacgacggcg aatttcgaat cgttacctga tgcactggct
    12061 tcccagcgtt tgaccgtagc gttaagtaaa tcaacaaaag cgatgttcac cgcttttcgt
    12121 caagtcgtgc catttgtcag ccaggatcgg gtctttaccc cggatattgc agcatcgcaa
    12181 aaattcttag agggatatgc tataaatc
//
LOCUS       DAZUGK010000134        12102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZUGK010000134 DAZUGK010000000
VERSION     DAZUGK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.135"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(62..970)
                     /gene="galU"
                     /locus_tag="ACHY5W_003273"
     CDS             complement(62..970)
                     /gene="galU"
                     /locus_tag="ACHY5W_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4448129.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1172..2185)
                     /gene="rssB"
                     /locus_tag="ACHY5W_003274"
     CDS             complement(1172..2185)
                     /gene="rssB"
                     /locus_tag="ACHY5W_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFS4448130.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVENPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2277..3182)
                     /gene="rssA"
                     /locus_tag="ACHY5W_003275"
     CDS             complement(2277..3182)
                     /gene="rssA"
                     /locus_tag="ACHY5W_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFS4448131.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3295..3753
                     /locus_tag="ACHY5W_003276"
     CDS             3295..3753
                     /locus_tag="ACHY5W_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFS4448132.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFAE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3803..4645
                     /gene="purU"
                     /locus_tag="ACHY5W_003277"
     CDS             3803..4645
                     /gene="purU"
                     /locus_tag="ACHY5W_003277"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFS4448133.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLYSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4805..4889
                     /locus_tag="ACHY5W_003278"
     tRNA            4805..4889
                     /locus_tag="ACHY5W_003278"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4839..4841,aa:Tyr,seq:gta)
     gene            4936..5067
                     /locus_tag="ACHY5W_003279"
     ncRNA           4936..5067
                     /ncRNA_class="other"
                     /locus_tag="ACHY5W_003279"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5251..5928)
                     /gene="narI"
                     /locus_tag="ACHY5W_003280"
     CDS             complement(5251..5928)
                     /gene="narI"
                     /locus_tag="ACHY5W_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS4448134.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLYSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5928..6638)
                     /gene="narJ"
                     /locus_tag="ACHY5W_003281"
     CDS             complement(5928..6638)
                     /gene="narJ"
                     /locus_tag="ACHY5W_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS4448135.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6635..8173)
                     /gene="narH"
                     /locus_tag="ACHY5W_003282"
     CDS             complement(6635..8173)
                     /gene="narH"
                     /locus_tag="ACHY5W_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS4448136.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8170..11913)
                     /locus_tag="ACHY5W_003283"
     CDS             complement(8170..11913)
                     /locus_tag="ACHY5W_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFS4448137.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVHDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGREHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2864 a         3288 c         2988 g         2962 t
ORIGIN      
        1 agctataaaa aaacggcgtc gattgctcaa cgccgtttcg tggataacac cgatacggat
       61 gttacttctt aatgcccatc tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt
      121 tatgacgaat accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat
      181 ggctcttccc tttcatatga taggcttcaa ccgtttcttt ttcgattagc atatcaattg
      241 cgtcggtgag ctgaatttca tcaccagctc ccggaggggt ttttgccagc aacggccaaa
      301 tatccgcact aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt
      361 ttggtttttc aaccacgcca accatcggta cgctttcacc cggcgctaat tcaacgcctt
      421 tgcaatccac aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat
      481 gacccgtttc atcgaagcgg cggatcatct ctgccaggtt atcctgtgat aaatcggatt
      541 catattcatc cagaataacg tcaggcaaaa taacagccac aggttcatca cccactaccg
      601 ggtgagcaca caataccgcg tgtcccaggc ctttcgccag cccctgacga acttgcataa
      661 tagttacgtg cggcggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt
      721 tttccagcat tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag
      781 atgagtgtgt aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt
      841 attgaattaa tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg
      901 gcaacatcct ggttcctaat cccgcaacgg ggataacggc ttttttgact ttcgtattaa
      961 tggcagccat ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat
     1021 cgcatcccag tatatcagca ccccggaggg gctaagttct gaaaaggatg cgagttagca
     1081 taattaagac aattacttat acgaatggcg acgatagtac caccagccga cattagctgg
     1141 taatgcaaat ttagcccgcg ttatcgtttg ctcattctgc agacaacatc aagcgcagcc
     1201 gaccaccggt tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca
     1261 aataagcgtt acctaaagtg cctaacggaa caccattact gatttgcacc tggtgttcgc
     1321 cggtatttaa cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat
     1381 gataatagcc aactaatagc ggaaactgcc ccggcagatt ggcctgacgg agtaaatggt
     1441 ttacctgctt caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt
     1501 cctgtaataa tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc
     1561 cagctcgggt gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat
     1621 caagcaccag gccgggtttg tccgcggcaa ccaattgacg ataattaacc cggcaatggg
     1681 aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcggcagggt
     1741 tttcaaccat tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgcgaattaa
     1801 acatgctggg atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaactg
     1861 gtttcagcaa aacatcttca acgcccagac gtaacgcttt ggcaatatct gccatatttt
     1921 cagtggcaga tatcaccaga actggggtct ggtcgcctct gttacgtata tgctccagca
     1981 gtttaagccc gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac
     2041 ctcccagcaa ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg
     2101 aggaaaacca cgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct
     2161 gttttccgac caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc
     2221 atgctattgc cacctgcgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa
     2281 atgttggtgc gtaccaacgg caaaagttcg tccattttct tttccactgc cagctgaccg
     2341 gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt
     2401 tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccagc
     2461 acctggatag aagtggtcat aatctctgtt gccgttggtg ctgtcaccgc ccgacgtgtc
     2521 gttatgctgc ctaacctttc tttcagacgc gcatgccacg gcagagaatc accattctcg
     2581 ctattttctt cactgacatt aaaggagagc aaatcttgtt gcatcaaatg agcatcgtgc
     2641 tgcaggtcga ccgctatcac aatatcagca cccaatgcac gcgtgagtga aataggaatt
     2701 gggttaacga ctgccccatc aaccagccag tagccgttat gcgcaacggg tgccatgagt
     2761 cctggaatac tgcatgatgc gcgaatagca agatggagat cgccttcagt aaaccataat
     2821 tcacgcccag tacttaaatt ggtggcaaca gccgcgaagc gacgggaaca attttcgatc
     2881 tctgtttccg gcattatttc gcggtattga ttgaagacac gctcgccgcg cagtaaccca
     2941 ccgcgctgcc aggagagatc catcaggcgt aaaacatccc aataactgaa agaggttacc
     3001 caatcttcca gcgcagataa tcgatcgcat gcataggcag cgcccaccag cgaaccaatt
     3061 gaacatcctg caacgatatc aatttcaata cccacttttt ttagcgcatt aataacgcca
     3121 atatgcgacc aacctctcgc cgcgccagat cccagcgcca gccctatttt tatctttctc
     3181 attattcccg caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa
     3241 catagtgcta ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct
     3301 cagctttgtc cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct
     3361 ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg
     3421 atgcaagacg cagattattt aataaagacc tggcatccat cttgtggagc agcggcgtta
     3481 cgtgccgaat tgattgccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa
     3541 cattgttggc aggacgccga caatatcgga tttgtcagtt ttgttgctcg atttgccgaa
     3601 ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg
     3661 tactatattg acggtacacg tccgcagttt ggccgcaacg atccctgccc ttgtggttca
     3721 ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac
     3781 tctcaacaag gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg
     3841 tccggaccaa aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa
     3901 tatcgtacag aacaatgaat ttgttgatca ccgtaccggg cgctttttta tgcgcacgga
     3961 actggaaggg atttttaatg attccaccct gctggcggat ctctatagcg cattgccaga
     4021 aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa
     4081 agaagcgcat tgccttggcg atttgttgat gaaagccaat tatggcggcc tggatgtcga
     4141 aatcgcggca gtgattggta accacgatac tttacgttct ctggttgagc gttttgatat
     4201 accgtttgag ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc
     4261 ggatgccatt gatgcttatc aacctgacta cgtggtgctg gcgaagtata tgcgggtatt
     4321 aacaccggaa tttgtgtcac gcttcccgaa taagatcatc aatattcacc attccttcct
     4381 gccagcgttt atcggcgcac gtccttatca ccaggcctat gaacgtggcg tgaagattat
     4441 tggcgcaacc gctcactatg tgaatgacaa tctggacgaa ggcccaatca tcatgcagga
     4501 cgttattcat gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt
     4561 cgagaaaaac gtcttaagtc gcgcgctcta caaagtactg gcgcagcgcg tctttgttta
     4621 cggtaatcgg acgattattc tttaatcgcc agcaaaaata actggttacc tttaatccgt
     4681 tacggatgaa aattacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg
     4741 tcatttgata tgatgcgccc cgcttcccga taagggagca ggccagtaaa aagcattacc
     4801 ccgtggtggg gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt
     4861 cgaaggttcg aatccttccc ccaccaccat cactttcaaa agtacctgaa ctctcaagcg
     4921 aatccgtaaa taaatattct gcccaagcgg ggaaggacga gaagcttcga ccaaggttcg
     4981 actcgagcgc cagcgagaga gcgttgccgc aggcaacgac ccgaagggcg aagcgcgcag
     5041 cgctgagtaa tccttccccc accaccattt cttcctgaaa tactcaaaac ctgttactcc
     5101 gagctaattc tgcccgatgg caccgcgttt accggaccta caatatcctc ccctgtaggt
     5161 cggataagcg aagcgcatcc gacaactcac cccgcaccgt gcccataaaa aaaccccgcc
     5221 gaagcagggt ctatgtgaac taaaattcgc ttagtgacga gcgcgcacca gctggtactt
     5281 acgtgtcaga tactccaccg gtacgctcca gatgtgtacc agacgcgaga acgggaacag
     5341 caggaataac gtcatcccca gcaccaggtg tagacggaag ataaacgcca caccatcaag
     5401 gtgttgagaa gcaccaccgt ggaaggtcac taccgactgc gcccagccaa ccagtttcat
     5461 catctcgcta ccgtccatat gctgggcgga gaacggaatg gtcagcagac ccaacgcgca
     5521 ctggataacc agcagcgaca ggatcaggat gtccgctccg gtagtcgttg cacgcacgcg
     5581 cgggctatac aggcggcgtt tgagcaatag cacgccgcca atcagacaca gcacgccgct
     5641 ggcaccacca gcaaacatgg ccattttctg tttcacttca atcggcagcc aggcttcata
     5701 catccagtgc ggcgtcagca taccgaagaa gtgaccgaca aaaatcccca gaataccgat
     5761 atggaacagg ttcgacgcca ggttcatccc tttgcgatcc agcatctggc tggacgccgc
     5821 gcgccaggtg tactgcccgt agtcataacg cagccagcta ccaatcagga agaccgcccc
     5881 ggctatatac gggtagatat caaagaagaa catattcagg aattgcatta gtgctgtcct
     5941 ccggtggtga tattcagata ttgcggcgca acggcaccgg caaagcgacg ctgatgagca
     6001 gtgattgctg aatcaccgca gcctttgtca gcaaagaatt taacctgctc ttcttcccag
     6061 acagcatcca gcgcctgcgg cgtatcatcg cgcgcttcgt cggcaatttt ttccgccact
     6121 ttgtcgctgt cgatagcggt attcgccagt ttcagcagca gatcaaacag cacggcataa
     6181 cggctttcac gctgttgcag acgcgcgctc agcaatgcca gaatcggcgc gatatctttc
     6241 aaaccttcca cggcttcgct ttgcggcagc tgcgccaggt actccagata cagcggcaga
     6301 tggtccggca attcgcggct gtttaactgc aagccgtgct gctcgtactg cgccagcagg
     6361 tccaccatcg cctggccgcg gtcgcgggat tcgccgtgca catgttcaaa cagcaacagt
     6421 gacgtggcgc ggccacggtc gaacagttcg ctgtactgcg cctgggcatc gagcggatcc
     6481 atcgtcgtta aatcgcgcag gaaaatgccc agcgcatggg catcctcttt tggcagattt
     6541 ttcgacgcgg caatcgcctc aaacatctct tgttgatgct gccataaggc agcatccgga
     6601 tattcaagga gacgcgatac aatcacgagt tcgatcatgg gtgcggctcc gttttgctgg
     6661 ttacgtcgat ggcatcgata cggcggctgt tgaacagatt gaatttggta tctgaaccgt
     6721 ggcagccatc accaaaggta aagccgcagc catttttctc cgggaaggct tcccgtgcca
     6781 gttcacgatg actactcggc accacaaagc gatcttcgta gttagcaatc gccagataac
     6841 ggtacatctc ctgtgcctgg gcttcggtca gaccgacctc ttccagcgca cgggtatcaa
     6901 ctttaccgtc aacggtttca gcacgtttgt aatgacgcat cgccagcata cgtttcagcg
     6961 cgcgcagtac cggtttggta tcaccggcgg tcagcagatt cgccagatac tgtaccggaa
     7021 tacgcaggct ttcgacgtct ggcagaatgc cgttgctacc cagctcaccc gcgtctgctg
     7081 cagactgaat cggagacaga ggcggcacgt accagaccat cggcagtgtg cgatattctg
     7141 gatgcagcgg cagcgccaat ttccattcca ttgccatttt ataaaccggc gactgctgtg
     7201 cagcttcaat aacgctcagc ggaataccgt ctttaatcgc ctgctcgatg actttcggat
     7261 cgttcggatc gaggaacacc tccagctgac gctggtaaag atctttctcg ttctcggtgc
     7321 tggctgcacg ttcaatagcg tcggcatcgt acaacagcac gccgagataa cggatacgac
     7381 cgacacaggt ttctgagcac acggtcggct gacccgcttc aatacgcgga tagcagaaga
     7441 tgcacttctc agacttaccg ctcttccagt tgaagtagat ttttttgtac gggcatccgg
     7501 tgatgcacat acgccagcca cggcatttat cctggtcgat caggacgatg ccatcttctt
     7561 cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca tgccgggttc aggcagtgtt
     7621 cgcacaggcg cggcaaatac atcatgaagg tgttttcgaa ctggctatac atcgccttct
     7681 ggatgttgtc gaagttcttg tctttcgcca gtttgtcaaa ctcaccgccc aggtcatctt
     7741 cccagttcgg ccctttttcg attttcgcca tccgttcccc ggtaatcagc gaacgcggac
     7801 gggcaatcgg ctgcgatttg ctgccttccg gcgcggtatg caggttctga tagtcaaaat
     7861 cgaacggctc gtaataatcg tcgatccccg gcagatgcgg gttagcgaag attttaccca
     7921 gcagcatggc acggttaccc atgcgcggct gcagtttgcc attgatttta cggatccagc
     7981 cgcctttgta tttttcctgg ttttcccagt cagtcgggaa gccctggccc ggcttggttt
     8041 ccacgttgtt gaaccacgcg tattccacgc cttcacggct ggtccagacg tttttacagg
     8101 taactgaaca ggtgtggcag ccgatgcact tatcgagatt cagcaccatg ccgacttgtg
     8161 aacgaatttt cattttacgc tctcctgtac ctggtcattg ccttcgccat ctaaccagtc
     8221 aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta
     8281 gtagttaaag ccgtatgcca gatgggcata gccgccgatc atatgcgtcg gtttcggcgt
     8341 gatacgggtg accgagttat ggataccacc acgctgttgg gtaatttccg aaccaggcag
     8401 gttaacgata cgttcctgcg cgtggtacat catggtcatc cctgccggaa cacgctggct
     8461 gacaaccgca cgggcagtca gagcaccgtt gctgttgaat acttcaatcc agtcgttatc
     8521 ggcgataccc agatctttgg catcggcttc actcaaccag accaccggac caccgcgacc
     8581 taaagtcagc atcagcaggt tgtcgctgta ggtggagtgg ataccccact tctggtgcgg
     8641 cgtcaggaag ttgagcgctt tttccgggtt gccgttggat ttctggccta tcacttcttt
     8701 caccgaacgg gtgtcgatcg gcggacgata aaccagcagg ctttcaccga aatcacgcat
     8761 ccactggtga tcctgataca gttgctgacg accagagagt gtacgccatg ggatcagctc
     8821 gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca tcttccagac cagaccaggt
     8881 cgggctggag ataattttgc gcggctgtgc ctgaatatcg cggaagcgga tcttctcgtc
     8941 ttctttattc agcgccagat gcgtatgttc acgaccggta aattcgctca gggcagccca
     9001 ggctttcacg gctacctgac cgttggtttc cggtgccagt gtcaggatca tctctgccgc
     9061 atcaattgcg gtgttcagca tcggctggcc tttcgccgga ccttccgctt tggtgtagtt
     9121 gagcttacgc agcagatcca tctcgctctg ggtgttccag gcaatccctt taccaccgtt
     9181 accgattttc tccatcagcg ggccgataga ggtaaagcgt tcgtaagttg ccggataatc
     9241 gcgctcaacc accataatgt gtggagcggt tttacccggg atcaggtcgc attcgccttt
     9301 tttccagtct ttcacatcca gcggctgcgc cagttcagcg gcagagtcgt gctggatagg
     9361 cagcgtgacg atgtcggttt ctttacccag atggccgacg cacacttcgg agaatttctt
     9421 cgcgatggct ttgtagattt cccagtcgct tttcgcttcc caggccggat cgactgccgc
     9481 agacagcggg tgaataaacg gatgcatatc cgaagtattc atgtcgtctt tttcgtacca
     9541 ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg ctcgacagac ggaagtccag
     9601 cgtaaccacc agatccagct tgccttccag accattgtcc tgccagtcca cttcttccgg
     9661 cttcacgccg ccctgctgcc ccagatcttt accctgtata ccgtgctccg tccccagcag
     9721 gtacttgagc ataaactcat gacctttacc ggaagaaccg agcaggttag aacgccagat
     9781 gaacaggttg cgcgggtggt ttttaccgtt ttctggttgt tctgccgcaa aacggatgga
     9841 accctctttc agggatttca ccgtatagtc caccggattc atcccggctt tttccgcttc
     9901 gccagcgata gtcagcgggt tagtgcctaa ctgcggtgca gacggcagcc agcccatgcg
     9961 ttctgcacgg acgttaaagt cgatcaggtg tccggtataa cgggatttat ccgccatcgg
    10021 cgacagcaac tcttccgccg tgacggtttc ataacgccac tggctggagt ggttatagaa
    10081 ataagaagtg ctgttcatgt gacgcgccgg acgctgccag tcaagggcaa acgccagcgg
    10141 ctgccagccg gtttgcggac gcagtttttc ctgacctaca tagtgcgccc agccgccccc
    10201 gctctgaccg acacagccgc agaaaatcag catgttgatc agaccacgat agttcatatc
    10261 gaggtgatac cagtggttca gccccgcacc gacgataatc atcgaacgac cgtgcgtttt
    10321 atcagcgtta tcggcaaatt cacgggcgat gcgaataatc tggctgcgag aaacgccggt
    10381 aatctgctcg gcccaggccg gggtataagc tttcacatcg tcatagctgg ttgcacagtt
    10441 aacgtcgttc aggccacgtt ccagaccgta gtttgccagc gtcagatcgt gaacggtggt
    10501 caccagagcg gtgctgccgt cagccagttg caggcgtttc accggcagct tgtgcaggag
    10561 cacgttttcc agttccactt tgttgaagtg ttccgtgccg tcgccaccaa agtacgggaa
    10621 gcccacctct gcgatctcat cctggctacc cagcaggctc agttgcagct cggtttcttc
    10681 gccagttttg ccgtcgcgct gctcaagatt ccatttgccc ttctcgcccc agcggaagcc
    10741 aatagaaccg ttcggcgcaa ccatttcgcc attggtatta aaggcgacag ttttccattc
    10801 cggattgttt tcctggccca gcgcatcaac cagatcagca gcgcgcagca tacgacctgc
    10861 agcgtagtaa ccgtcgcgtt cttccagcat caccagcatc ggcatgtcgg tgtagcgacg
    10921 cacatagtcg gtgaaatact ggcttgggtt gtcgaggtgg aattcacgca gcattacgtg
    10981 gcccatcgcc agcgccattg ccgcatcggt gccctgtttc ggtgccagcc acagatcgca
    11041 cagtttggcg atttcagcgt agtctggtgt gacggcaaca gttttggtgc ctttgtaacg
    11101 cacttcagta aagaagtgag catccggggt acgcgtctgc ggcacgtttg aaccccaggc
    11161 gatgatgtag ctggagttgt accagtcagc agattccggt acgtcagttt gctcgcccca
    11221 agtttgcgga gacgcaggag gcaggtcgca gtaccagtcg tagaagctta agcaagtacc
    11281 gccaatcagc gagagatagc gtgcacccga tgcgtaagaa accatcgaca ttgccggaat
    11341 tggcgagaaa ccagcaacac ggtccgggcc gtagtttttg atagtgtaaa cgttagatgc
    11401 ggcgatcagt tcgttcacct cctgccagga agaacgaaca aaaccaccgc gtccacgcgc
    11461 ctgcttaaag cttttcgctt tatcggcgtc ttcaatgata gaagcccatg cctcaaccgg
    11521 atcgctatgc agcgccttcg cttcacgcca cattttcatc aggcgtttgc gcatcatcgg
    11581 gtatttcagg cggttggcac tgtaaagata ccaggagtag ctggcaccgc gcgggcagcc
    11641 gcgaggttca tggtttggca gatccggacg ggtacgcgga tagtcagtct gctgggtttc
    11701 ccaggtgacc agaccgtttt tgacgtagat tttccagctg caggagccgg tgcagtttac
    11761 cccgtgggta gagcggacga ttttgtcatg ctgccaacgc tggcgatatc catcctccca
    11821 gtcacggttg gtattgagaa gctggccatg cccatcggca aaggtttcac ccttctgttt
    11881 gaagtagcga aaccggtcca ggaatttact catcggtttt ctcctgtggg agcctgtcgg
    11941 ccctctgata aatcgacatt gctaatgttt aagggcaagg taacgctctg aaacagcgtg
    12001 ggaattgata acgatcaaga gtgatgggga tgaaaaataa agtaaattcc taagataccc
    12061 ctttaggata attaacagtt acagcataac atgctgtaaa tt
//
LOCUS       DAZUGK010000135        12084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZUGK010000135 DAZUGK010000000
VERSION     DAZUGK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.136"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(345..1079)
                     /gene="cysH"
                     /locus_tag="ACHY5W_003284"
     CDS             complement(345..1079)
                     /gene="cysH"
                     /locus_tag="ACHY5W_003284"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS4448138.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1153..2865)
                     /gene="cysI"
                     /locus_tag="ACHY5W_003285"
     CDS             complement(1153..2865)
                     /gene="cysI"
                     /locus_tag="ACHY5W_003285"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFS4448139.1"
                     /translation="MSEKHPGPLVVEGKLADAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AADNTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIERIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2865..4664)
                     /gene="cysJ"
                     /locus_tag="ACHY5W_003286"
     CDS             complement(2865..4664)
                     /gene="cysJ"
                     /locus_tag="ACHY5W_003286"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFS4448140.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRLERRYQRDVY"
     gene            4983..5345
                     /gene="queD"
                     /locus_tag="ACHY5W_003287"
     CDS             4983..5345
                     /gene="queD"
                     /locus_tag="ACHY5W_003287"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFS4448141.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5423..6694
                     /gene="ygcN"
                     /locus_tag="ACHY5W_003288"
     CDS             5423..6694
                     /gene="ygcN"
                     /locus_tag="ACHY5W_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4448142.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6685..6945
                     /gene="ygcO"
                     /locus_tag="ACHY5W_003289"
     CDS             6685..6945
                     /gene="ygcO"
                     /locus_tag="ACHY5W_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS4448143.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            6962..7537
                     /gene="ygcP"
                     /locus_tag="ACHY5W_003290"
     CDS             6962..7537
                     /gene="ygcP"
                     /locus_tag="ACHY5W_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFS4448144.1"
                     /translation="MPFLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7684..8544)
                     /gene="ygcQ"
                     /locus_tag="ACHY5W_003291"
                     /pseudo
     CDS             complement(7684..8544)
                     /gene="ygcQ"
                     /locus_tag="ACHY5W_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(8541..9320)
                     /gene="ygcR"
                     /locus_tag="ACHY5W_003292"
     CDS             complement(8541..9320)
                     /gene="ygcR"
                     /locus_tag="ACHY5W_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFS4448145.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQNYLRQRMQP"
     gene            complement(9298..10707)
                     /gene="ygcS"
                     /locus_tag="ACHY5W_003293"
     CDS             complement(9298..10707)
                     /gene="ygcS"
                     /locus_tag="ACHY5W_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4448146.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHSIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10729..>12084)
                     /gene="ygcU"
                     /locus_tag="ACHY5W_003294"
     CDS             complement(10729..>12084)
                     /gene="ygcU"
                     /locus_tag="ACHY5W_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFS4448147.1"
                     /translation="DRFRKFPDIHGIYTLPIPAAVVKLGSTEQVSRVLNFMNAHKING
                     VPRTGASATEGGLETVVENSVVLDGSAMNQIINIDIENMQATAQCGVPLEVLENALRE
                     KGYTTGHSPQSKPLAQMGGLVATRSIGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNV
                     PRRAAGPDIRHIIIGNEGALCYITEVTVKIFKFTPENNLFYGYILEDMKTGFNILREI
                     MVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYP
                     QCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVI
                     NRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKII
                     CEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIE
                     K"
BASE COUNT         2759 a         3332 c         3094 g         2899 t
ORIGIN      
        1 tttttttagt attatcgatg atatcagcgg gctatttttc gatgctgctc ataaatatcg
       61 aattacagtg atatacacat gtcttgcctc cctgcaaaga agagactaac atccgaattg
      121 cttaggttcg aacgctggcc tcaggttgat agaaatatcg cctggggctt ttgtccatct
      181 ggaacctcgc gaatgcttaa cgccagacag cctcaagcac ccaacgccat tctatacctg
      241 ttaattcttc cagcggcgtt cacgtttaaa atcaggaata ctgctcgcaa aataaaaacg
      301 cccccatcat ttctgacaga ggcgtttaat ttatccggca atatttaccc ttcgtgtaac
      361 ccacattccc tttttaagcc aaagaaacgc gtttcttctt ccgccatgcc gggttcccat
      421 ttacgggttg tatgggtatc gcccaccgat aaatatcctt cgtcccataa tgggtgatat
      481 ttcaggccat gtttttgcag gtactgataa atagttcggt tatcccagtc gataatcggc
      541 agcactttaa atacgccacg ctgaattgcc agcaccggta aattggcgcg gctgccggat
      601 tgttcacggc gcaggccagc aaaccaggtt tgcgcgttaa gttctttcag cgcccggttc
      661 atcggttcga ctttgttgat gtcattgtac ttttcaatgc cttcaacgcc ctgctcccac
      721 agttttccgt agcgtgcttc ctgccaggct gcgctttcgg tagcacggta cactttcagg
      781 ttgagcttga gtttgtccgt taactcgtca ataaagcggt aggtttccgg gaacaagtaa
      841 ccggtatcgg tgaggatcac tggaatatcc gggcgaatct gattcaccag atgcaggctc
      901 accgccgcct gaatgccgaa gctggaagaa agcacatatt caccgggcag attatccagc
      961 gcccaggcta cgcggccttc agcgtccagt ttttccagtt gggcgttagt ttccgccagc
     1021 gccagaatgc gatctacctt cggcagttcg ttcagggcgt ttagatcgag tttggacata
     1081 gattcctcac tgtttgcctt gcctgatgcg acgctcacgc gtcttatcag gcctacaaga
     1141 ccgggctgat ggttaatccc acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc
     1201 cgcacgcacc gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg cccagcgccc
     1261 tatcagttca tcaagcgacg ccaggatttc cggctcggtg atgttttctt tatacatccg
     1321 tgggatacgt gtcccaatac ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt
     1381 acccaccagg cccacttccg ccagcatcgc gcgaccacaa ccgttcggac agcctgttac
     1441 acgcatcacg atatgctcat cgctgacacc atgtttcgcc attaaattat cgatgttgtc
     1501 gataaaagac ggcaggaaac gctccgcttc cgccatcgcc agcgggcaag tcgggaatga
     1561 cacgcaggcc attgagtttt cacgctgcgg cgtgacggca ttcattaacc cgctctcttt
     1621 ggcgatcctc tcgatcttcg ctttctcact ttccggcaca ccggcgatga tcagattctg
     1681 gttcgccgta atgcggaaat cacctttgtg gatcttcgcg atctccagca ggccggtttt
     1741 cagcggacgc cccggataat caaggatgcg accattttcg ataaacagcg tcaggtgcca
     1801 gttatcatca atgcccttaa cccagccaat acgatctccg cgtccggtaa actcatacgg
     1861 gcgaatcggc tcgaatttga tccccgcgcg acgttccact tccgctttaa atgtctcaac
     1921 acccacgcgc tccagcgtgt atttggtttt ggcattttta cgatcggtac ggttacccca
     1981 atcgcgctga gtcgtcacga cggcttccgc caccgccagg gtatgctcca gcggcagata
     2041 accaaactcg ctcgccgtgc gggcgtaggt tttcttatta ccgtgttcga tggaaagccc
     2101 gccgcccacc agcaggttaa agcctaccag cttgccgttt tcggcgattg ccacgaagtt
     2161 catgtcgttg gcgtgcagat cgatatcgtt ctgcggcggg atcactaccg tggttttgaa
     2221 tttacgcggc aaataagtct gaccgaggat cggttcttca tcggtggtgg cgactttttc
     2281 ctggtcgagc cagatctccg catacgcgcg ggttcgcggc agcagatgct cagagatctt
     2341 cttcgcccac tcgtacgctt ctgcgtgcag ttgcgactca tacgggttcg aggtgcacaa
     2401 cacgttacgg ttcatatcgt tggcggtcgc caacgcatca agaccgaccg agtgcagcat
     2461 ctggtgcacc ggtttgacgt tcttcttcag aataccgtga aactggaatg tctggcggtt
     2521 ggtcaggcgg atgctgccgt aaatcgtgtt atcggcagca aacttatcaa ttgcctgcca
     2581 ctgtttagtg gtgattaccc cacccggcag gcggcagcgc agcagcatcg catgacgcgg
     2641 ttccagcttc tgttcagcac gttcggcgcg aatgtcgcga tcatcctgct gatacatgcc
     2701 gtggaaacgg atcagcagga agttatcgcc cttaaacccg ccggtcaggc catcgtttaa
     2761 atcttcagca atcgtgccgc gcaggtagtt gctctcaagc ttcatgcgct ccgcgtctgc
     2821 cagtttacct tcgaccacta aaggccctgg atgtttttcg ctcattagta gacatctcgc
     2881 tgataacggc gctctaagcg cagctcactt aaaaattcat ccgccgcttc ggtgtccatg
     2941 ccaccaaatt cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc catgcgatta
     3001 gcgtcgccgc agacataaat gtgggcaccg tcattgatcc agcgccacag ctccgcgccc
     3061 tgttcgcgta gtttgtcttg tacgtaaact ttttcttttt gatcgcgcga ccaggcgaga
     3121 tcgatacgtg tcagcacgcc ctctttgacg tagcgctgcc actccacctg gtagaggaaa
     3181 tcttccgtaa aatgcgggtt gccaaagaac agccagtttt tacccggtgc ttcatcggca
     3241 gcacgttgct gcataaaggc gcggaacggc gcgataccgg tgcctgggcc aatcataatc
     3301 accggggttt ccgggttagc gggcagacgg aagttatcgt tatgttcgat aaatacgcgg
     3361 acttcgccct cttcttccac gcggtcagcg aggaagctgg aggcaccacc ggcacgggcg
     3421 cggccttcca cgtcgtaacg caccacacca acggtgacgt gtacttcgtt ctcgacttcc
     3481 gcctgcgagg aggcaatgga atagagacgc ggcgtcagcg ggcgcagcag attaattagc
     3541 gcttcggcat caagctgtgc cggggagaaa cgcaccatgt caacaatcgg cgtcgtcgcg
     3601 gcgtaatgct gtaacttcgc tttatcgccc accagcggca gcagtgtttc gctgcgggta
     3661 agcgtggcgt aattctcaac aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt
     3721 agcgcttcgt tcagaggcaa cgttttgccc tcgacggtga caggttcatc gcctttcagc
     3781 cacagcagtt cgacaagttc tttcaccagt gccggatcgt tctgatacca gacacccagc
     3841 gcgtcacccg gctggtaacg caggcccgag tcacctaagt caatttcgat atggcgaacg
     3901 tctttttcag agttacgccc ggtaattttc tggttaaccg aaaggctcgc caccagcggc
     3961 gcgtctttgc tgtacgggct ggtgtggatt tcatttacca cgccagtagc gacggattgc
     4021 gaaggtgccg cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc gcgccactcg
     4081 ctggcagcag cctggtattc aacgtcggca tcgacacggt cgagcaggcg ttcaccaccc
     4141 agttccgcca gcttgctgtc aaaatctttc ccggactggc agaaaaattc ataagagcta
     4201 tcgccgaggc taaacacggc aaatgcggtg ttttccagct tcggcgcttt tttggagaac
     4261 aggaacttat gcagcgcgac ggcttcttcc ggcggttccc cttctccttg cgttgacgtc
     4321 accacgataa gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc
     4381 agcttaacgt tcagttttgc cgctaataaa tcatcacgta atgcttcagc aacccggcgc
     4441 gcattgccgg tttgcgaggc ggagataata gttatacccg gcatttctgc ggcgggagct
     4501 ggcgtcgctg caagcgcagc aggctgctga ttgagcacgc cccagaaata gccagaaacc
     4561 caggcaagct gggtgggagt taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc
     4621 gggttcaacg gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca
     4681 gtaagcaaag ctgtttctgc gccctgtcag cgcccataaa acagaagaga aggtaaggtt
     4741 aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta
     4801 acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacctaat gaaaaacaaa
     4861 tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata
     4921 atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta
     4981 tgatgtccac cacgttattt aaagatttca cattcgaagc cgctcaccgc ttaccacacg
     5041 tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa
     5101 ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa ctgaaagcag
     5161 cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt ccaggcctgg
     5221 aaaacccgac cagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc
     5281 cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc tatcgcggcg
     5341 aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg
     5401 ttctggatat ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg
     5461 tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact
     5521 ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc gggcggttat atacccatgc
     5581 actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac gcatcactca
     5641 cgaaagcctt tccctgttaa cgccggattg cgcaacgacg ttttccagct tacagcccgg
     5701 cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga
     5761 aaaagaaggt gtcgaatgca tccctggtgc gacggtggat gcactgtatg aagaaaacgg
     5821 cagggtgtgt ggtgtcattt gtggtgacga tattctccgc gcccgttatg tggtgctggc
     5881 agaaggtgcc aacagcgtcc tggctgaacg tcacggatta gtgactcgtc ctgctggcga
     5941 agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg
     6001 ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggga tctgtgatga
     6061 cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag gaattgtttg
     6121 tccactctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctgg ctcgctttaa
     6181 aacgtatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca
     6241 tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggct
     6301 gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatgga
     6361 tatggcgctg actggcgcac aggcggcggc acaaacgctg ataagcgcct gccagcaccg
     6421 cgagccgcaa aatctgtttc cgctttatca tcacaacgtc gagcgcagcc tgctgtggga
     6481 tgttctacaa cgttatcagc atgttccggc gcttttgcaa cgcccgggat ggtaccgtgc
     6541 gtggcctgcg ttaatgcagg atatttcccg cgatttatgg gatcagggtg ataaacctgt
     6601 tccaccgctg cgccagttat tctggcgtca tttacgtcgt cacggcctgt ggcatctggc
     6661 gggcgatgtt atcaggagtc tgcgatgtct gtagcccgta atctctggcg cgttgctgat
     6721 gcgccgcaca ttgttccggc tgactccgtt gagcgccaga cggcagaacg gttgattaac
     6781 gcctgtccgg caggtctttt ttcgctcaca ccagaaggtg acttacgtat tgactatcgc
     6841 agttgcctgg agtgtggcac ctgccgtttg ctgtgcgacg aatcaacact acaacagtgg
     6901 cgctatccgc cttccggatt cggcatcacc taccgttttg gataaataat aaggataatt
     6961 tatgcccttc ttacacctgc tccgccagaa tccggtgatt gctgccgtta aagataatgc
     7021 cagcctgcaa ctggctatcg attctgaatg ccagtttatt tccgtgttgt acggcaatat
     7081 ctgcaccatc agtaatatcg ttaaaaagat taagaacgcc gggaaatatg cttttattca
     7141 tgtcgatttg ctggaaggcg cgtcaaataa agaagtcgtg attcagtttt tgaaactggt
     7201 gaccgaggcg gatggaatta tcagcaccaa agcatcgatg ctaaaagcgg caagagcgga
     7261 aggttttttc tgtattcatc gcctgtttat tgttgattcg atttcgtttc acaacattga
     7321 taagcaagtt gcgcaatcga atccggattg tattgagatc ctgccaggct gcatgcccaa
     7381 agtgctgggc tgggtgacgg aaaaaatccg ccaaccgctg attgctggcg ggctggtatg
     7441 cgatgaagaa gatgcgcgta atgcgattaa cgcgggtgtc gtggcgctgt ccaccacgaa
     7501 taccggggtc tggacattag cgaaaaagtt gctttgacgg gatattcgta acgaattgaa
     7561 tttgtgtgat ttgtaggctg cacgccacat ctgacattca gcgcctgatg cgacgcttga
     7621 cgcgtcttat caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac
     7681 acgttattgg caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc
     7741 atcaaccacg ccaacatctg cctgtgaaaa taccgctgca ctggcgtcat gattaatcgc
     7801 caccacaaac ttgctattgc gtacgcccgc cattaacgct gccgcaccgg atgcgccaac
     7861 aacaatacac acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcggcatc
     7921 aacgccaccg ttcatcactc gtgcccgact gtagcctact tcggctccca gcttttctgc
     7981 cagcatagca atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct
     8041 ggcttcagct aatgggtcgc ggctaacatt tttcagatcc tctgtgctaa ccagccagtc
     8101 gggaagagca cctgggacaa tgttcagttg ctgcatacca gagggcaacg tggcattttt
     8161 atctgctccc gcctggcgcg ccagggaaag acatagcggg cgtttttctg tttgcagcgt
     8221 cgccgttagc gcatttccct agtgggattt gcgcacgctt acggttggaa tctctaacga
     8281 agtcacctgg cagatgcttg caccatgtaa actccaggcc agccgggtcg acagttcatc
     8341 gccaaacgtt cccggcggaa agagaacaac gttcgcaggc gttcgctgcc actgttccac
     8401 cagcgcatcc agcacctgtt ccgccactac gggctgaggt tcaatctgcc agtgcgccag
     8461 ggtacaaccg ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt
     8521 tattgtcaca attgcgatat tcatggctgc atcctttggc gtaaatagtt ctgccatagc
     8581 ttttgcgctt tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct
     8641 gctcttgcca gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata
     8701 atttctgctt tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca
     8761 ccgcactgac gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caacccatgt
     8821 tcagtacgct gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg
     8881 aagcagggcc agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg
     8941 ctgctttggc agccggtaat tatcagatcc agcggattct gacgctgcca ttcggcaata
     9001 tggcgagcga caaattccgg tgcaaagcgc agatctgccg ccgtctccag caatacagct
     9061 tcctcaaacc caagagccat gagatagcgc agccagtgca acgcccgttc atcccccatg
     9121 ctcaacgcgg ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggcg
     9181 gcagcctgtt catcagcacc gagtaaactt cgcagtagcg aaacatccgg tccgctatta
     9241 ccctgagccg ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta
     9301 aacgctaaca gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagtgc
     9361 tttggtttct ggtgcccata gccaggtcac gacaaaacca actaacaaca ctgccgccag
     9421 gatcaataag gtaacctgca ttccccactg cgccagcacc cacggcagca ggccagtact
     9481 taccgccgcg ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc
     9541 agtaggaaaa ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt
     9601 gctgaagaga acaaaaagca gtagcgttaa tgaactgccg gaaggcaaac aggccatcac
     9661 taccagcgtt gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag
     9721 gtgcgtcaga actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct
     9781 ggcagtcagc gcatcttcca gtccaatagt ctgggcgata gttggcagcc aggtataaat
     9841 cacaaaccat gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca
     9901 gtagcgcgaa gagaacaagg ttttgatgtg tttatgggtc gccgttgcca cttcatcgcc
     9961 cagtaaaaca tggggaccaa aatagcgatg cacgatagag tgagcttctg caaaacgccc
    10021 ctggcgcagt agccagcgtg gcgattctgg cgttccccag cgtaataacg taatcaacaa
    10081 tgcgggcaga gctgccgatg ccagcagcca gcgccaggcc tccgggcttt cgctaataaa
    10141 gtgatgtccg gcaatacttg ccaacacata gcctacggtc cacaccacgc tgaatgcgcc
    10201 cagcaaaata ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga
    10261 gtaatcgcct cccagaccaa tgccgatcaa aatgcgcagc ccaataagat gctctggcgt
    10321 ggtggcaaaa aattgcaaga acgaagcgag cgtaatcagc aaaaagctga aggtgaagat
    10381 tttttgccga ccaatatggt cggagatcca cccaagaacc aggctgccaa gaaacaaacc
    10441 aaggagcgcc gagccgccta tcattcccgc cataaacggc gtcagttgca tggcgggcgt
    10501 aagttgaata atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc
    10561 gaaagtgagc gcagcaattc gacagtgaaa acggttaagc ggtaaatcat ccattcgcac
    10621 cggtgaagtg ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga
    10681 gaagccgaaa aggcaacgcc ccatcatcgc ggaacgctgc ctgacacatt atttttcaat
    10741 cggatagata gtacccgtat tcataatgcc gttaggatcg aactgctttt tcagcccttc
    10801 cagcaacgcc cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat
    10861 accgtggtgg tgcaccatcg aaccaccgag gcgaatggtt tcttcgcaaa tgattttgtt
    10921 gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacaa cgttgtaatc
    10981 gtagacgaag tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat
    11041 ggtgatgtcg tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat
    11101 ttcgtggatg cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt
    11161 gaggatttgc acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca
    11221 ggtttcgatc agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc
    11281 aatcccttcg cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac
    11341 acattttccg tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg
    11401 agcgatcgac ggacgatacc cttccaccat gatttcacgc aggatgttga agccggtttt
    11461 catgtcttcc aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt
    11521 cactgttact tcagtgatat agcacaatgc accttcgttg ccgatgatga tgtgacgaat
    11581 gtccgggcca gccgcgcggc gtggcacgtt tttaatacgt gtgacggtgc catcagccaa
    11641 tacggcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc
    11701 gatactgcgg gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg
    11761 ccccgtggtg taaccttttt cacgcaacgc gttttccagc acttccagcg gaacaccaca
    11821 ttgcgccgtc gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc
    11881 gtcgagcacc accgagtttt ctacaacagt ttccagccca ccttcggtgg cggaagcacc
    11941 ggtacgcggc acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg
    12001 ttctgtggaa ccgagtttta cgaccgcagc cggaatcggc aaagtataaa taccatgaat
    12061 atccggaaat ttacgaaaac ggtc
//
LOCUS       DAZUGK010000136        12031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZUGK010000136 DAZUGK010000000
VERSION     DAZUGK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.137"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            370..1758
                     /gene="narU"
                     /locus_tag="ACHY5W_003295"
     CDS             370..1758
                     /gene="narU"
                     /locus_tag="ACHY5W_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFS4448148.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIVVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1840..5580
                     /gene="narZ"
                     /locus_tag="ACHY5W_003296"
     CDS             1840..5580
                     /gene="narZ"
                     /locus_tag="ACHY5W_003296"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFS4448149.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLNNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGDCDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQSPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5577..7121
                     /gene="narH"
                     /locus_tag="ACHY5W_003297"
     CDS             5577..7121
                     /gene="narH"
                     /locus_tag="ACHY5W_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS4448150.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTYDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVLQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7121..7816
                     /gene="narW"
                     /locus_tag="ACHY5W_003298"
     CDS             7121..7816
                     /gene="narW"
                     /locus_tag="ACHY5W_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS4448151.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYSLFDALLHLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7813..8493
                     /gene="narI"
                     /locus_tag="ACHY5W_003299"
     CDS             7813..8493
                     /gene="narI"
                     /locus_tag="ACHY5W_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS4448152.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8572..9465
                     /gene="yddE"
                     /locus_tag="ACHY5W_003300"
     CDS             8572..9465
                     /gene="yddE"
                     /locus_tag="ACHY5W_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFS4448153.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9561..10406)
                     /gene="nhoA"
                     /locus_tag="ACHY5W_003301"
                     /pseudo
     CDS             complement(9561..10406)
                     /gene="nhoA"
                     /locus_tag="ACHY5W_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10579..11148
                     /gene="yddH"
                     /locus_tag="ACHY5W_003302"
     CDS             10579..11148
                     /gene="yddH"
                     /locus_tag="ACHY5W_003302"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4448154.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11152..11379)
                     /gene="pptA"
                     /locus_tag="ACHY5W_003303"
     CDS             complement(11152..11379)
                     /gene="pptA"
                     /locus_tag="ACHY5W_003303"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFS4448155.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHMNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11478..11930)
                     /locus_tag="ACHY5W_003304"
                     /pseudo
     CDS             complement(11478..11930)
                     /locus_tag="ACHY5W_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2819 a         3117 c         3128 g         2967 t
ORIGIN      
        1 agaccttctg gacagatcgt tttcctccag ctcatggcct tttgagtgaa taaattattg
       61 ctaattctat aagtgtcttg tcttgttaag aatatagtta atatacaacc ctttggattc
      121 atcatctgaa gggttgtatc atgacatgcg ttcaaagatt accccccgtt taatggttag
      181 cttgtcagtt acatttagta acacatatct ggctacctaa tccccattct gcccccgcgg
      241 ttacgctttc ttatctattc tttcaatcag cacttcaccc aattcatcct taattctaat
      301 aactggctat tgcgccttac ctcctgtttt gtcttttttc attaccaatg tgtgcatgtg
      361 aggaacaata tggcactgca aaatgagaaa aatagtcgtt atcttttacg cgactggaaa
      421 ccagaaaatc cggccttctg ggaaaataaa ggaaaacata ttgctcgaag aaacctctgg
      481 atatcagtca gttgtctact tcttgccttc tgtgtctgga tgctatttag cgcagttacc
      541 gttaatctca ataaaatcgg ttttaatttt actaccgatc aactcttttt attaaccgca
      601 ttaccctccg tttctggcgc attattgcgt gttccctact cctttatggt acctatattc
      661 ggtggacgcc gatggacggt ttttagtact gcaatcctga ttattccttg cgtctggctc
      721 ggaattgtcg tgcagaatcc gaatactcct tttgggatct ttatcgttat cgctttgcta
      781 tgcggttttg caggtgcgaa ctttgcttcg agcatgggca atatcagttt cttctttcca
      841 aaagccaggc aagggagcgc tcttgggatt aatggcggat taggaaactt aggtgtaagt
      901 gtgatgcagc tggttgcacc gctggtcatt tttgtacccg tatttgcctt tctcggcgtc
      961 aatggcgtcc cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc atggatttgg
     1021 gtaccgctac tggcgattgc cacgatcgcc gcctggtcag ggatgaatga tatcgccagt
     1081 tcacgcgcgt caattaccga ccagctccct gtcttacaac gcctgcatct ctggctgctg
     1141 agcctgcttt accttgccac cttcggttcg tttatcggtt tttctgcggg ttttgccatg
     1201 ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc tggcgttctt tggcccattt
     1261 atcggtgcca tcgcgcggtc ggttggtggt gctatttccg ataaattcgg cggcgtgcgg
     1321 gtgacgttga tcaactttat ttttatggcg attttcagcg ccctgctgtt ccttacctta
     1381 ccgggcacag gctccggtaa tttcatcgcc ttttacgccg tatttatggg gctgtttctg
     1441 accgcgggtc tgggaagtgg ttctactttc cagatgatcg ccgtcatctt tcgccagata
     1501 accatttatc gggtgaagat gaaaggcggt agtgatgagc aagctcaaaa agaagccgtc
     1561 accgaaacgg cggcggctct gggctttatc tcagccattg gcgcagtggg cggctttttt
     1621 attccgcagg cgtttggcat gtcgctcaat atgaccggct ctccggtagg cgcgatgaaa
     1681 gtgtttttaa tcttctacat cgtttgtgtg ctactgacct ggctggttta tggtcggcgg
     1741 aagttcagcc aaaaataaga gatcgatgcc tgatgtacaa ccgtgtcgca tccgacaagg
     1801 catcagatcc caattcattc ctggagcagg agtcatgtca tgagtaaact tttggatcgc
     1861 tttcgctact tcaaacaaaa gggcgatacc tttgccgatg gtcacggaca ggtgatgcat
     1921 agcaaccgcg actgggagga cagctatcgc cagcgttggc agttcgacaa aatcgtgcgt
     1981 tccacccacg gtgttaactg tacaggctcc tgtagctgga aaatctacgt taaaaatggt
     2041 ctggtgacct gggaaatcca acagaccgac tacccgcgca ctcgccctga cctgcccaat
     2101 catgaacctc gcggctgccc gcgtggcgca agttactcct ggtatcttta cagcgctaac
     2161 cgcctgaaat acccgctcat tcgtaaacga ctgattgaac tgtggcgcga agccctcaaa
     2221 caacacagcg atccggtact ggcgtgggca tcgattatga acgatccgca aaagtgcctg
     2281 agctacaaac aagtgcgtgg gcgcggcggg tttatccgct ccaactggca ggaactaaac
     2341 cagctgattg ccgccgctaa cgtctggacc atcaaaacct acggcccgga tcgcgttgcc
     2401 ggtttctcgc cgatcccggc gatgtcgatg gtttcttacg ccgccggaac acgttatctg
     2461 tcactattag gtggcacctg tttaagcttc tacgactggt attgcgacct gccgcccgcc
     2521 tcgccgatga cctggggcga gcaaaccgac gtaccggaat ctgccgactg gtataactcc
     2581 agttacatca tcgcctgggg gtctaacgta ccgcagacac gtacgccgga cgcccacttc
     2641 tttaccgaag tacgttacaa aggcaccaaa actatcgcca ttacccctga ctactctgaa
     2701 gtggccaaat tgtgcgacca gtggctggca ccgaaacaag gcactgatag cgccctggcg
     2761 atggcaatgg gccatgtgat tttaaaagag tttcatctca ataatcccag cgactacttt
     2821 atcaactact gccgccgcta cagcgacatg ccgatgctgg taatgctgga gcctcgcgac
     2881 gatggtagct acgttcccgg gcgcatgatc cgcgcatctg acctggtgga tggactgggc
     2941 gaaagcaaca atccgcagtg gaaaaccgta gcagttaata ccgcaggtga attggtagtg
     3001 ccgaacggtt cgattggttt ccgctgggga gaaaaaggca aatggaatct ggaatccatt
     3061 gccgccggta cggaaaccga attgtcgtta accctgctcg gtcaacatga cgctgttgca
     3121 ggcgtggcct tcccctactt tggcggcatt gaaaatccgc attttcgcag cgtaaaacac
     3181 aatccggtgc tggtgcgcca attgcccgtt aaaaacctga cgttagtcga tggcaacacc
     3241 tgtccggtgg tcagcgttta tgatttggta ctggcgaatt acggcctcga tcgcgggctg
     3301 gaagatgaaa acagcgcgaa agattacgct gaaatcaaac cgtacacccc agcctggggt
     3361 gagcaaatta ccggcgtacc gcgccagtat attgaaacca tcgcccgcga atttgccgat
     3421 accgcccata aaacgcatgg gcgctcaatg attatcctcg gtgctggcgt caaccactgg
     3481 tatcacatgg atatgaacta ccgtgggatg atcaatatgc tgatcttctg cggttgtgtc
     3541 gggcaaagcg gcggcggctg ggcgcactat gtcggtcagg aaaaactgcg cccacaaacc
     3601 ggctggttgc cgctggcctt tgcgctcgac tggaaccgcc caccgcgcca gatgaacagt
     3661 acctcgtttt tctacaatca ttccagccaa tggcgctatg aaaaagtctc tgcgcaggag
     3721 ttactttcac cgctcgccga tgccagtaag tacagcggtc atctgattga tttcaacgtt
     3781 cgcgccgaac gtatgggctg gctaccttct gcgccgcagc tggggcgtaa cccgctcggg
     3841 attaaagctg aagccgacaa ggccggatta tcccccacag aatttaccgc ccaggcgctg
     3901 aaatcgggcg atttacgtat ggcctgcgaa caaccagata gcggcagcaa tcatccgcgt
     3961 aatttgtttg tctggcgttc taacctgctt ggctcctcag gcaaaggcca cgagtatatg
     4021 cagaagtatc tgctggggac cgaaagcggg attcagggca aggaactcgg tgccagcgac
     4081 gggatcaaac cggaagaagt cgagtggcaa actgcggcga ttgaaggcaa gctcgacctg
     4141 ctggtgacgc tcgacttccg catgtccagt acctgcctgt tctccgatat cgttctgccc
     4201 accgccacct ggtatgaaaa agacgatatg aacacctcgg atatgcatcc atttattcat
     4261 ccgctttctg cggcggtcga tccggcctgg gaatcacgca gcgactggga aatctacaaa
     4321 ggtattgcca aagcattttc gcaagtgtgt gtgggtcatc ttggcaaaga aaccgacgtg
     4381 gtattgcaac cactgctgca cgactctccg gcagagctct cacagccgtg tgaagtgctc
     4441 gactggcgca aaggcgattg cgatctgatc ccgggtaaaa ccgcgccgaa tattgtggcg
     4501 gtggagcgcg actaccctgc tacgtatgaa cgctttacct cgctcgggcc attgatggac
     4561 aaacttggca acggcggtaa agggatttcg tggaatacgc aggatgaaat cgatttcctc
     4621 ggtaaactca attacaccaa gcgtgatggc ccagcgcagg ggcgtccgct gattgacacc
     4681 gccattgacg cttcagaagt gattctggca ctggcaccag aaaccaacgg tcatgttgca
     4741 gttaaagcgt ggcaggcgct gggcgagatc accggacgcg aacataccca tctggcgctg
     4801 cacaaagagg acgagaagat tcgctttcgc gatattcagg cgcagccgcg taaaattatc
     4861 tccagcccca catggtccgg tctggaaagc gatcacgtat cctataacgc gggatacacc
     4921 aacgttcatg agttaattcc gtggcgcacg ctgtcgggac gccagcagct ctatcaggat
     4981 catccgtgga tgcgtgcttt tggtgaaagc ctggtggctt atcgcccgcc tatcgacacc
     5041 cgtagcgtca gtgagatgcg ccagagcccg ccaaacggct tcccggaaaa agcacttaac
     5101 ttcctgacgc cgcaccagaa atggggcatt cactcaacct acagtgaaaa cctgctaatg
     5161 ctgacactct ctcgcggtgg accgattgtc tggatcagcg aaacagatgc ccgagaattg
     5221 accattgtcg ataacgactg ggtggaagtg tttaacgcca acggcgcgct gactgcccgc
     5281 gcggtggtca gccaacgtgt accgccgggt atgaccatga tgtatcacgc tcaggaacgc
     5341 attatgaata ttcctggttc ggaagtaact ggcatgcgcg gcggtattca taactcggtc
     5401 acccgcgttt gtccgaaacc aacgcatatg attggcggtt acgcacagct ggcctggggc
     5461 tttaactact acggcaccgt cggctcgaac cgcgacgaat tcatcatgat ccgcaagatg
     5521 aagaacgtta actggctgga tgatgaaggt cgcgatcagg tacaggaggc gaaaaaatga
     5581 aaatccgttc gcaagtcggc atggtgctta atctcgataa atgtatcggc tgccatacct
     5641 gttcggtgac ctgtaaaaac gtctggaccg gacgcgaagg catggagtac gcatggttta
     5701 acaacgtcga aaccaaaccg ggcattggtt atccgaaaaa ctgggaagat caggaagagt
     5761 ggcaaggcgg ctgggtccgc gatgtgaatg gcaagatacg cccgcgtctg ggcagcaaga
     5821 tgggcgtaat aaccaaaatc ttcgccaacc cggtggtacc gcagattgat gattactacg
     5881 aacctttcac ctacgactac gaacatttgc atagcgcacc ggaaggcaaa catatcccta
     5941 ctgctcgccc gcgttcgctg attgacggta agcggatgga caaagtgatc tgggggccaa
     6001 actgggaaga actgctgggc ggcgagtttg aaaaacgtgc ccgcgaccgc aacttcgagg
     6061 ccatgcaaaa ggagatgtac gggcagtttg aaaacacctt catgatgtac ctgccgcgcc
     6121 tgtgcgaaca ctgcctcaat cccagctgcg tggcgacctg cccaagcggc gctatctaca
     6181 aacgcgaaga agacggcatt gtgctgattg atcaggataa atgccgtggc tggcgtttgt
     6241 gcattagcgg ttgtccgtac aaaaaaatct acttcaactg gaaaagcggc aagtcagaaa
     6301 aatgtatctt ctgttaccca cgaattgagt ccggtcaacc gaccgtgtgc tcagaaacct
     6361 gcgtgggtcg catccggtat ctgggcgtgc tgctttacga cgccgaccgc atcgaggaag
     6421 cggcgagcac cgagcgcgaa gttgacctct atgaacgcca gtgcgaagtg ttcctcgatc
     6481 cacacgatcc ctcagtgatc gaggaagccc tgaaacaagg tattccacaa aatgtgattg
     6541 acgctgccca gcgttcgcct gtctacaaaa tggcgatgga ctggaaactg gcgctaccgt
     6601 tgcaccctga ataccgcacc ctgccgatgg tctggtacgt tcctccgctg tcaccgattc
     6661 agtcttacgc tgatgcgggc ggtttgccga aaagcgaagg cgtgctaccc gccatcgaaa
     6721 gcctgcgtat tccggtgcaa tatcttgcca atatgttgag tgccggtgat accggtccgg
     6781 tactgcgggc gctgaaacgg atgatggcta tgcgccacta tatgcgttca caaaccgtgg
     6841 aaggcgttac tgatactcgt gccatcgacg aagtaggcct gagcgtcctc caggtcgaag
     6901 agatgtatcg ctatctcgcc attgccaact atgaagaccg ttttgtcatt ccaaccagtc
     6961 atcgggaaat ggcgggcgat gccttcgctg aacgcaacgg ctgcggtttt acctttggcg
     7021 acggttgtca cggctcggac agcaaattca acctgttcaa cagtagccgt atcgatgcca
     7081 tcaacatcac cgaagtgcgc gacaaagcgg agggcgaata atgcagatcc tcaaagtgat
     7141 cggtctgttg atggagtatc cggatgagct gttgtgggaa tgtaaggagg acgcgctggc
     7201 gttgatccgc cgcgacgcgc cgatgcttac ggatttcacc cgcaacctgc ttaacgcgcc
     7261 gctgctggat aaacaggccg aatggtgcga agtgtttgac cgcgggcgca ccacgtcgct
     7321 gctgctgttc gaacatgttc atgccgagtc ccgcgatcgc ggccaggcaa tggtggacct
     7381 gctggcggag tatgaaaagg tcggcctgca gctggattgt cgggaactgc ccgattatct
     7441 accgctgtat ctggagtatt taagcgtact gcctgacgat caggcgaaag aaggattgct
     7501 caatgttgcg ccgatcctcg ccctgcttgg cggtcgctta aaacaacgcg aagctccgtg
     7561 gtattcgttg tttgacgccc tgctgcatct ggcgggaagc atactttcaa gtgacagcgt
     7621 cacaaaacaa gtgaatagcg aggagcgcga tgacacccgc caggcgctgg atgcggtgtg
     7681 ggaagaggaa caggtgaagt ttattgaaga taatgccacg gcgtgtgaca gctcgccgct
     7741 taatcaatat cagcgacgct ttagccagga tgtcgcgccg caatatgtcg acatcagtgc
     7801 gggaggtggg aaatgattca gtatctgaac gtcttttttt acgatatcta cccgtacatt
     7861 tgtgcgacgg tgtttttcct cggcagctgg ctgcgctacg actacggcca gtacacctgg
     7921 cgcgcctcct caagtcaaat gctcgataaa cgcgggatgg tgatatggtc gaatctgttc
     7981 catatcggca ttttggggat cttcttcggg cacctgttcg gcatgttaac gccgcactgg
     8041 atgtacgcgt ggtttttgcc agtggcagcg aaacaactaa tggcgatggt gctcggcggt
     8101 atttgcggtg tgttgacgtt gattggcggc gcaggactgc tgtggcgcag gctgacaaat
     8161 cagcgcgtgc gtgccacttc cactacgccg gatatcatca tcatgagcat tttgctggtc
     8221 cagtgtctgt tggggctaag caccataccg ttttcagcac agtatcctga tggtagcgaa
     8281 atgatgaagc tggtgggttg ggcgcaaagc attgtcactt tccgtggtgg atcgtcagag
     8341 atgcttaacg gtgtagcgtt cgtcttccgc ctgcatctgg tgttgggaat gaccatcttc
     8401 ctgctcttcc cgttcacccg actggtgcat gtgtggagcg ccccgtttga gtactttact
     8461 cgtcggtatc agattgtgcg ctcgcggcga taatttcacc ctggccggat agcccttcgc
     8521 tctccggcca cttgcaggct atgatagttc tccagtcaca ggagaacgtt aatgaaaccg
     8581 caggtttacc acgtcgatgc ttttacctct caaccatttc gcggcaattc tgccggtgtt
     8641 gttttccccg ccgataatct tagcgaagcg caaatgcagc ttatcgcccg cgaattaggt
     8701 cattcggaaa ctgcttttct gctgcacagc gacgacagcg atgtgcgcat ccgctacttt
     8761 acgccaacgg ttgaagttcc gatttgtggt cacgcgacag tagctgcgca ctatgtacgt
     8821 gcgaaggtgt taggtttagg aaattgcacg gtctggcaaa catcgctggc aggaaaacat
     8881 cgcgtgacta tcgaaaagca ccacgatggt tatcgtattt cgctggaaca aggtacgccg
     8941 ggctttgagc caccgctgga aggtgaaaca cgtgcggcga ttatcaacgc gcttcatctc
     9001 actgaggacg atattctgcc aggcttgcca attcaggtgg caacaacagg acactcaaaa
     9061 gtgatgatcc cgttgaaacc agaagtggat atcgacgccc tttcgcctga tcttgctgcg
     9121 ctgagcgcta tcagcaaaca gattggctgc aatggtttct tcccattcca gatccgccca
     9181 ggcaaaaacg aaaccgatgg tcgtatgttc tcgcctgcga ttggtattgt ggaagatccg
     9241 gtgactggca atgccaatgg cccgatgggt gcatggttgg tacatcacaa cgtattgccc
     9301 cacgatggca acgtgttgcg tgttaaaggc catcaggggc gcgcattggg gcgtgacggc
     9361 atgattgaag tgacggtgac aattcgtgat aaccaaccgg aaaaagtcac catttcaggc
     9421 acagcggtga ttttgtttca tgccgaatgg gcaattgaac tctgaaaccg ttgtctgatg
     9481 cgcttcgcct gtcaggtcta caaccgggct aatgtatgcc ggataaggcg tttacgccgc
     9541 atccggcaac ctgacataaa ttattttccc gcctccgggt gcgtatcaaa cgccgccatc
     9601 accagcgcta actcatccac ggtaaagcca tgtttcgcat catccacgcc cagaccaaac
     9661 tgttcttgca tcacggcata taacgacgcc acatccggta gatttcgctg ctccaccgcg
     9721 tgcccatttt cataatgggt aaaatgaaaa ttggtcagtg tcagcttgcc gccgtccggt
     9781 aaatggcggc acatcagcaa atgatggcga aaatgcgact gcggccagtg cgccgaccag
     9841 aatttgccca tcacataatc gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac
     9901 tgccagtgct gatgatgatt aaactacaac acccaattat caccctcctg caacaaccga
     9961 tactctccgt gtggcgtggt ctgcacgaga tcgggaacta aacgaatcgg cgcggttagc
    10021 gtctgcccac cgaaaccgac atcagcaatc catttttcct cttccagttc caccaacagc
    10081 aaacgatggg tgcgcggcgg taatgctggc ggatttgata acactacgcg ccctaacaag
    10141 ctgcgaacgt taaaccccag ctcgcgtaac acccgctcaa acacgccatt ctgctcaaaa
    10201 cagtaaccgc cacgacgggc tatcaccagt ttctcttcca gcgattgatc atcaagctgc
    10261 atttccctcg gcagcaaaac gtcgaggttt tcaaacggaa tggtgcaatt gtgtttcagg
    10321 tgcaatgcac gaagcgtatc aatattgacc gcagcagttc ccgaccagtt aatacgggca
    10381 aaatagtgat tcagaatggg cgtcatcagg cgtttccctt agtggttttc tcaacttata
    10441 acctggaata gacgccgcac cggctatttt gtgcgatcgc ggacatgcat cacgttcgca
    10501 aatcgccaat cccgttaatt tcgttactta ttgcgttacg gactacgctt aaaaaaacct
    10561 tcttcgcgag gcgataccat gagccgattc atccccatag aattacacca tgccagccgt
    10621 ctgttaaatc atggcccaac ggtcttgatc accagtttcg atgagcaatc ccagcggcgt
    10681 aacattatgg ctgcagcctg gtcaatgccg gtggagtttg aaccgccacg cgtcgcgatt
    10741 gtggtagata aatcgacatg gaccagggag ttgattgaac gtaacggtaa gtttggcatc
    10801 gttatcccgg gcgttgcagc aactaactgg acgtgggcgg tgggaagtgt gtcggggcgt
    10861 gatgaagata aatttaattg ctatggcatt ccggttgtga gaggcccggt atttggtttg
    10921 cctctggtcg aggaaaaatg tctggcgtgg atggagtgcc gcctgctacc tgcgacttct
    10981 gcgcaacaaa aatacgatac gctgtttggc gaggtggtat cagcagcggc agacgcacgg
    11041 gtatttgtcg aaggccgctg gcagtttgat gatgataagc tcaatacgtt gcatcattta
    11101 ggtgctggca cgtttgttac cagcggcaag cgtgttacgg caggttaagc attacatgct
    11161 gtaaccgggt ttctttatca aagcctccat ttggggcgcg atttcggcat cccagatagc
    11221 ttgccaagat tctggttgaa tctgctgtag agcaatgctt atcgaactgt ctttactgtt
    11281 catatggcga ataataacgt cggtaatatc tgcagcaagt gctgcttttt gttgttcgtc
    11341 cagttcacgc ggaaaacatt taatgtctat gtgcggcatg attacgctcc ttttgaaaga
    11401 tgtcagtaaa attaacacac attgtgaatg tcgtgtgtcc ggtaatgcgt ttttacgaca
    11461 ataatgccag ggcaagatta cgaaagcctt ctccccgcaa ggactgacgc caggtagttt
    11521 ctgtccatgg ctgcttttcg catcttacgt cttaacccag ccttgaatac cttatcattc
    11581 gtcaaaatat taatagcaat gtgccgtatc cctgaaaata attctgctgc atttcctctt
    11641 cttattttgc agtcgtcttc attcattacc acgtccagac gccagtgcag cttattctcc
    11701 acgtgccagt ggtttcggat tgctgtggcg aacttctctg cggttaaatc agcagaactg
    11761 atataatatc tgaccgtcat ttctggctct ttcttttgtt ctgctattat ggaccgaaag
    11821 gagactgcca cgcataattt cttcagccct ttccattcaa acgtgaaatc aataagttca
    11881 tcaggaacat cgcaaacaat atgaagacgg atttcttctc tgccgtgact aaaatgcgaa
    11941 ttatttagta caaaaaaagc ggcgcacctt gttctttccc agattcctaa tatggcaact
    12001 ttaagttatc gccgtctata cttatagaaa t
//
LOCUS       DAZUGK010000137        11962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZUGK010000137 DAZUGK010000000
VERSION     DAZUGK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.138"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..1026
                     /locus_tag="ACHY5W_003305"
     CDS             4..1026
                     /locus_tag="ACHY5W_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFS4448156.1"
                     /translation="MSLTRRRFTQILASTLLMHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLSKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1026..2006
                     /locus_tag="ACHY5W_003306"
     CDS             1026..2006
                     /locus_tag="ACHY5W_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4448157.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIIYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2003..2761
                     /locus_tag="ACHY5W_003307"
     CDS             2003..2761
                     /locus_tag="ACHY5W_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4448158.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSLLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2771..3583
                     /locus_tag="ACHY5W_003308"
     CDS             2771..3583
                     /locus_tag="ACHY5W_003308"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4448159.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3580..4434
                     /gene="modD"
                     /locus_tag="ACHY5W_003309"
     CDS             3580..4434
                     /gene="modD"
                     /locus_tag="ACHY5W_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFS4448160.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAIDQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4460..6430
                     /locus_tag="ACHY5W_003310"
     CDS             4460..6430
                     /locus_tag="ACHY5W_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFS4448161.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYCGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6480..6734)
                     /gene="ymgE"
                     /locus_tag="ACHY5W_003311"
     CDS             complement(6480..6734)
                     /gene="ymgE"
                     /locus_tag="ACHY5W_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4448162.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6935..7669
                     /gene="ycgR"
                     /locus_tag="ACHY5W_003312"
     CDS             6935..7669
                     /gene="ycgR"
                     /locus_tag="ACHY5W_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFS4448163.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7671..8282)
                     /gene="emtA"
                     /locus_tag="ACHY5W_003313"
     CDS             complement(7671..8282)
                     /gene="emtA"
                     /locus_tag="ACHY5W_003313"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFS4448164.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8382..9296
                     /gene="ldcA"
                     /locus_tag="ACHY5W_003314"
     CDS             8382..9296
                     /gene="ldcA"
                     /locus_tag="ACHY5W_003314"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFS4448165.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEELNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHYFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTREGTQLTISGHPVLKM"
     gene            9391..11127
                     /gene="cvrA"
                     /locus_tag="ACHY5W_003315"
     CDS             9391..11127
                     /gene="cvrA"
                     /locus_tag="ACHY5W_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFS4448166.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLISLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11230..11382)
                     /locus_tag="ACHY5W_003316"
     CDS             complement(11230..11382)
                     /locus_tag="ACHY5W_003316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448167.1"
                     /translation="MVDGDTGNAGLVILHLLQSEEAITLTFLSRCFEFIGQSLQWFVI
                     HNLTFF"
     gene            11777..>11962
                     /locus_tag="ACHY5W_003317"
     CDS             11777..>11962
                     /locus_tag="ACHY5W_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448168.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQII"
BASE COUNT         2796 a         3141 c         3065 g         2960 t
ORIGIN      
        1 cttatgtcac taaccagacg gaggtttaca cagattcttg cgtcgacgtt gttgatgcat
       61 catctgcctt cttttgcaca atcagtcaaa ttctgggcct cactgacgct tcccgaagcc
      121 caaaacatta cacgcatcgt cagcgcaggt gcgcccgccg atttgctatt gctggctgtc
      181 gcgccagaaa aaatggtcgg tttttcctct tttgattttg cccgtcaggc attaattcca
      241 ttgccagagc acattcgcca gttacccagg ctgggacgac tcgccgggcg cgccagcaca
      301 ctctcgctgg aagggcttat ggcgttacat cccgatttgg tcgttgattg cggcaatacg
      361 gatgaaacct ggatctccca ggcacggcag gttagcgaac agacacaaat accctggtta
      421 ttgcttaacg ggaaactgga gcaatcagca gaacagttaa caacgcttag caaaacgtta
      481 ggcgaagagc atcgcgccgc agaacaagcc aatctcgcca gccgcttcgt tggtgaagct
      541 caggcattcg ccacctcacc cgccgctaac ctccgctttt atgctgcgcg cggtcctcga
      601 gggctggaaa cgggcttaaa gggttcgttg cataccgagg cggcggaatt attaggtttg
      661 cacaacgtcg cgcaaatagc cgatcgccac ggcctgacac aggtttccat ggaaaatctc
      721 ctgcgctggc agccggatat tattctggtt caggaggccg ttactgcaga ctttattcgt
      781 cgtgatcctc tctggcaggg cgtgaaagcc gttgcggaac aacgcatctt atttttaagt
      841 ggcttgccct ttggctggct ggatgcccca ccgggaatca accgtcttct gggattacgc
      901 agacttcacg cctggctgga tcccgccatc aatcgccagt ttaaaagtga catgcagcat
      961 tacgcccaac tgttctggca ttgttcactc agtgacgccg actatcaaaa attggtggcg
     1021 agctaatgag aatcgtaaac ggatgtattt tgctgctggc agctatcagt atcacttttg
     1081 ccgccgtaag tggggcttat catctcgata tgcagcaact gctggcgcta attcttcgtc
     1141 aagaaaatgt tcctgtgcag gaacaaattg tcttctggca aatccgcgtg ccgcgtattc
     1201 ttgctgcgct gttactgggc gcggcgctgg caggagccgg aaccacctat cagggaatgt
     1261 tgcgtaatcc cttagtttca ccggatattt taggcgtctc cgccggtgcc ggacttggtg
     1321 cctgcacggc aattctgtgg ggattatcca ttatttacat ccagctgtac gcattttgcg
     1381 gcgggctgat ggtggtcgcg ggcgtctggt tgattacgcg tcgggtgacc cgccacgatc
     1441 cgatcttaac cctggtgctg gtaggcattg cgttaggaac gttgtgcgga gcggggatat
     1501 cgctaattaa aacgctggcc gatccttaca cccagttacc gtcaattacc ttctggttgc
     1561 tggggggact gtccaccgtg accttacgcg atctctgcta tgctgcaccg atcattctca
     1621 taggctcctt gcccctgttt tttctccgct ggcgcatgaa tttactcacg ctttccgacg
     1681 acgaagcccg ttcgctggga ctgaacgtga cgcgtctgcg ctttgtattg attgtctgcg
     1741 ccacactaat caccgccagt acggtagcaa tagcgggcat aattggttgg gtcggcctgg
     1801 tcgttccgca cattgcccgc ttgttgaccg gtcataacca tcagcagctg ttgcccatgg
     1861 caatgtgtac tggcgcgatt ctgctcctgc ttaccgatac gctggcacgc agcattggaa
     1921 caacagaaat cccacttggc attctgaccg cttttgttgg cgcaccgttt ttcctgcttt
     1981 tgttgttacg cggaggtcgc cagtgacttt gcttgccgtc cgtcatgcgt ctctgggtta
     2041 ctcccgccat ccggtgttac gcgacgtgtc cttcaccctg tcgcaaggaa caatttgttg
     2101 tctgttagga gccaatggtt gcggcaaaac cacactcatg cgctccattc ttggcgttat
     2161 accgttatta aaaggcgagg tcttactgga tagcctccct gtgcagacac tcagccatcg
     2221 ccagcgcgcg caggctattg cctgggtgcc gcaggcgcac gacggtattt ttgcttttag
     2281 tttgctggat atggtgttga tgggcctggc ccccaccata ggcgcttttt ccgttcccgg
     2341 taagcaagaa cgcctgaaag ccacggagca actggaaaag ctgggtattt tgcatctggc
     2401 ggcacgacgc tggaatacgc ttagcggcgg tgaacgtcag ctggctttaa tcgcccgtgc
     2461 actcgtccag caaccgcgct tgttgttact ggatgagcct gcctccagcc tcgatttcgg
     2521 tcatcagatc caactactgg acacgctggc gcagctcaaa aataacggca tgacaatgtt
     2581 aatgtcgact catcatccgc tccatgccaa cgccattgcc gacagcatta tccaggtaga
     2641 gccagatgga cgagtaacac aaggcttacc gaccgaacag ttaacaacaa acaaactggc
     2701 tgccctgtat cgggtttctg ccgaccagat tcatcatcac ctctctgcaa ttagccacta
     2761 aggaaaaatc atgctcattg atgatattga ttttgcagac ctttatctgc agcaactcaa
     2821 gctggcccac cgcacagaaa aaacgccgga tcactgggat caacgggcgg aaaaaatggc
     2881 ggaaaattgc gcctcgccta cggacagcta cctgcaacaa ctgatctcaa aaattgacct
     2941 acaaggcgct cagaccttat tcgatatggg ctgcggaccg ggtaccgtat cgctggcgct
     3001 ggctgacaaa ctcacgacga tttatggcgt tgattacagt caggggatgc tgaacgttgc
     3061 cgcccgtcgc gccgccgcgc tgaaggcaga caatgtccac tggatccagc gcgcctggga
     3121 agaggactgg agtgacctcc cacgctgcga tattgccgtg gcttcccgct ccactctggt
     3181 ggctgatatg cgccaggcaa tgagcaaact gaataatcag gcacggcttc gcgtctatac
     3241 gactcatctg gtcagcacct cgttcgtctc tccagccatt cagcgggcgg ctggtcggga
     3301 agtgatcgaa ttacccaact atatctttgc gctcaacgtc ctgtatcaga tgggaattta
     3361 tgcccacgtt gattttatcc gtggacaaaa ttgccaacag gataacagca cctgggaacg
     3421 ttttgagcag aacgtcagct ggagtcttgg cgcgcttaac gacgatgagc gtgagcggtt
     3481 atatcgctgg tatcaacaac aagatgctcg tgccctcgcc cctgccagcc gcgactgggc
     3541 gctcatctgg tgggatagcg taccgcagga ggcactgcga tgattttcct ttctcaggca
     3601 caaatcgatg cgttacttct ggaagatatc cagggcggtg acctgaccac gagagcatta
     3661 aatattggac accagcatgg ctatatagag ttttttctcc gtcagggcgg ctgcgtcagc
     3721 ggcgtttctg tcgcgtgtaa gatgttaact acgctgggat taactattga tgacgcggtc
     3781 agcgacggtt cacaagcgaa cgcgggtcag cggctaatcc gtgcgcaggg taatgccgca
     3841 gcacttcatc agggatggaa ggcaatccag aatgtgctgg agtggagttg cggtgtttct
     3901 gattatctcg atcaaatgct ggcgttactt cgtgaacgtt atcctgacgg caatatcgcc
     3961 tgcacccgaa aagcaattcc gggcacccgc ttactggcct cgcaggcaat tctggctgcc
     4021 ggaggactga ttcatcgtgc cggatgtgcg gaaaccatat tattgtttgc caaccaccgc
     4081 cattttcttc atgacaatca ggactggtca ggcgcaatcg atcagttacg tcgccacgca
     4141 cccgagaaga aaattgttgt tgaagccgac acgccgaaag aggcaatcgc cgcgttacgc
     4201 gcgcaaccag acgtacttca gctcgacaaa tttagtccgc aacaggcaac tgaaattgcg
     4261 caaatagcgc cgtcgctggc tccccactgc acgctggctc tgaccggcgg aattaatctg
     4321 acgacactca aaaattacct cgactgcggc attcgcctct ttattacctc cgcgccttat
     4381 tacgcggcac cggctgacat taaagtcagt ctgcaacccg cagcctctat ttaattcttt
     4441 gttaaacgga atttattcta tgagactcaa aaaacattat ctctgcacag cattatcact
     4501 tgcctttacc cagcaggccg tagcggctca ggagagcgac tcgctgaccg tatggtccag
     4561 tccggtatca tcgacgacga ccaccgttct cgatcaaccc accatgaagg ccctggataa
     4621 acagaatgtc gctcaggcat taagtgtcgt ccccggcgtg gtgctgcaaa agtcaggcag
     4681 ccgcaacgaa gaacaggtaa aagttcgtgg ctttgatagt cggcaggtgc cggtctattt
     4741 cgacggtgtg cccatttatg ttccctatga cggcaacctc gatctggcgc ggattctgac
     4801 caacaatctg ggggcagtcg aagtttccaa agggtattcg tcgctgcttc aggggcctaa
     4861 tcagatgggc ggagccatta atatcaccac ccagaagcca acaaaacctc tggaagcaag
     4921 tctgggatat cgccagggat ggagccgtag ccaggacaat gcctacgata tgcatgcttc
     4981 atttgccgcc agtagcgatc tggggtattt gcaagtcagc ggtagccagc taaagcagga
     5041 ttttctcggc ctgccgcatg gtgtaaataa tgatattgca ggcaaacacg gcaagatgat
     5101 taattcatcg gctgatgata aacgcggcat tgtgaagctc ggatttacac cacgtgaaaa
     5161 cgatgaatac acactgactt acattaagca ggatggtgaa aaagataacc cgccatactg
     5221 cggaaatagt ggtcaaaaat cacgctactg gcagtggcca gagtatgaca aagaaagttt
     5281 ttattatcag ggaacgaccc aactaaacga tcgttttacc ctgaaaagtc ggctgtatcg
     5341 cgacaccttt gaaaatacgc tgatgatgta caactcgctg gctgatttga aaaataaaaa
     5401 aggcagctac agccattatt ccgattacag cgacggtgcc ggattacaac tggcagccga
     5461 tgtgcgtgaa aacgatctgc tgtcgtttgc cgttaactgg aaagatgacg tacaccggga
     5521 aaaaggtgcg ccgcacgccg cttacgatcg ctatgaagat cgtacctggt cgctcgccag
     5581 tgaatatcaa tgggctgctg ccgataatgt cgatgtcgtg gctggaatca gctatgactg
     5641 gcgcgatagc gtagaagcga aaaaacacga gaaagatggc agcatcaccc attatgacga
     5701 caacaatcag tcagctttta actggcaggt gatggggaaa taccactttg tcaatgaaga
     5761 cacgctggcg ctttcgtact atgaccgcac acgctttccg acgctgaaag aacgctatac
     5821 cacatccaaa cctgcgtata accagatagc gattgttaac ccgcagctca agccggaacg
     5881 cgctcgcggg gtggatttaa cctggaatgg tgccttcacg cgcgactggg gatttgaggt
     5941 cagcgtttac tataaccggg tgagtgatgc catcctctcg cacaatatcg atgccgatac
     6001 cattcaaaat cagaacagcg gcacggtgga ttacagcggt ctggatgccg gtattaaggg
     6061 gaaaatcagc aatatactgg atgtaggatt gagctacgcc ctgatccacg ctgacgccaa
     6121 acgcaaagac atcggcaaga taaccgatct gccaacgcag acaatgaccg catggatgac
     6181 actcaaacca tgggagccgt taagcgtaac gctgtcggaa gaggcgcgat cctccagcta
     6241 cagcaacagt gacggttcac aaaaagccgc cggttttgcg gtgacccaca ttcgagccga
     6301 ttacacctta ggtcatggct tcagcgtcaa tgcgtcggtc aataacctgt ttgataccca
     6361 atatgcctac agtgaagggt ttattgaaga aggccgcaat ttctgggcag gtgtcgagta
     6421 tacgttctga tgtgaaagcg gatagcggca tctgtatcgc tatccgcctt atgaaaatct
     6481 tattctcttc gcaggaggcg gaatacgccc agaacgagaa tagctcccac caccgccacc
     6541 aggaagctgt gcaaattaaa accgctgatg gagccgccaa tgccaaacat ggtcgccagc
     6601 cagccgccga ccaccgcacc gactatcccg agaatacagg tcaggaaaaa tccaccacca
     6661 tcacgccccg gcatgattag cttggcgata atgccggcta tcaggccaaa aataatccag
     6721 gcaataattc ccatgttcag gccctcgttt cgatgaatcg gacgcaagaa atgcttacat
     6781 caacgttcta agtataggca agggcgggat cgatgctggg cgtatgtcgg ctgatttaag
     6841 aacaacatgt aaaaaatatt cacgcatttg tattaagttt tgttaactgt gaccgataaa
     6901 ccaaagacag tttgtcagtc aggagttttt ccgcatgagt cattaccatg agcagttcct
     6961 gaaacaaaat ccgttagccg tcctgggcgt gttacgcgat ttgcacaaag ccgcaattcc
     7021 tttgcgtatc agctggaatg gcgggcagct gatcagcaaa atactggtaa taaccccgga
     7081 taaactggtg ctggatttcg gcagtcaagc cgaagacaac atcgccgtac taaaggcaca
     7141 gcacattacc attaccgccg aaactcaggg tgcgaaagtc gagtttactg ttgaacaact
     7201 acaacagagt gaatacttgc agcttccggc atttattacc gtaccgcctc ccaccttatg
     7261 gtttgtacaa cgacgccgat atttccgcat ctccgcccca ctccatccgc cttatttttg
     7321 ccagaccaaa ctggcggata acagtacgtt acgtttccgc ctgtatgatt tgtcgttagg
     7381 cggcatgggc gcattactgg aaacagcaaa gcctgccggg ttgcacgaag gtatgcgctt
     7441 cgctcagatt gaagtcaaca tggggcaatg gggtgttttt cactttgacg cccagttaat
     7501 ctccatcagc gagcgcaaag tgattgatgg caagaatgaa accatcacca ctccccgtct
     7561 gagcttccgt tttcttaacg tcagcccgac ggtggagcgg caattacagc ggattatttt
     7621 ctctctcgag cgggaagccc gggagaaagc ggacaaagta cgcgactgat ttacatcgcg
     7681 tccagtgcct gctcaagttt gtagatatag cgcggagcct gcggcgcagg atgatttcgc
     7741 gctacgtgat cgaggaactc gtcagcatcc agatcgttga ttttgctgat tgcctttttc
     7801 cgatctgacg agaaagtccg tagcagcgca cctgccccgt tagcgtatga caccaccagc
     7861 gcatattgca gtaccttcgg atcttcaatg cctgccagcg ggccggtttc cagaatattc
     7921 aggtaagccg cccccattga aatattacgc tccggatttt tcagctcgct ggtcgtcggc
     7981 tcaccactcc agcccatacg ccgataaaca tcacgtccgg aggttgaagc ttttaactgc
     8041 atcaaaccaa tggcattcga tttactcacc gcgttcggat taccacccga ttcgatagcg
     8101 ataatcgccg tgatcaattg tggatcgacg ccccaggctg caccggcttt ctggcttatt
     8161 ggcatccact gcatcgcacg ttgcaccgga actttcgcgt tccacggcgg gttcgtgtag
     8221 tcatgctttg atgaacaacc cgctaataac acaatcaaaa aggcaaacca tctcaatttc
     8281 acttcatcta tccttatagc ctggaatcgc cgttgaggtg acaacggcac acctaagcgg
     8341 catgatatac atttcgtttc tgttgtcagc aaggaattgc catgtctctg tttcacttaa
     8401 ttgccccatc gggttactgc attaaacagc acgccgcgct gcgtggtatc cagcgcctta
     8461 ccgacgcggg gcatcaagtc aataacgtcg aggttattgc ccgtcgttgt gagcgttttg
     8521 cgggtacgga aacggagcgt ctggaagaac tcaattccct tgccagactg actaccccca
     8581 acaccatcgt gctggctgta cgcggcggtt acggtgccag tcgtttactg gcagatattg
     8641 actggcaggc actggtggct cgccaacaac atgacccgtt gctcatatgc gggcatagcg
     8701 attttaccgc cattcagtgc ggtcttctgg cgcagggcaa tgtcatcacc tttagcggcc
     8761 ccatgctggt agcaaacttt ggcgcggatg aactgaacgc ctttaccgag cactatttct
     8821 ggctggcgtt acgcaatgaa accttcacca ttgaatggca aggggaaggc ccgacatgtc
     8881 agaccgaagg cacgttgtgg ggaggcaatc ttgcgatgct gatttcactg attggtacac
     8941 cgtggatgcc aaaaattgag aacggtattc tggtgctgga agatattaac gagcaccctt
     9001 tccgcgtcga acgtatgctg ttgcagctct atcatgctgg aattttgcca cgtcagaagg
     9061 cgattattct cggtagcttt agcggcagta cgcctaatga ttatgacgca ggttacaacc
     9121 tggagtcagt gtacgcgttt ttgcgttccc gcctgtcgat tccactcatt accggcctcg
     9181 attttggtca tgaacaacgc acggttacgc tgccgttggg cgcacatgcc atgcttaata
     9241 acacccggga aggcactcag ttgacgatta gcggtcatcc tgttcttaaa atgtaaaaaa
     9301 atcacgcttc agggctaagt aaaacgctgt ctctgccgct aatatggtag ggtttatata
     9361 agagacagcg taatcaggag taaccgaccg ttggatgcca caacaataat tagccttttc
     9421 atcttaggat ccatccttgt caccagcagt atattactta gttcattttc ttcccgtctt
     9481 ggcattccta ttctggttat ttttctggcg atcggtatgc tggcaggagt cgatggcgtc
     9541 ggcggtatcc cgtttgataa ttaccccttc gcctacatgg taagtaacct ggcgctggcg
     9601 attattttgc tcgacggcgg gatgcgcact caggccagtt cctttcgtgt ggcgttagga
     9661 ccggcgctgt cgctggcgac gctgggcgta cttatcacct ctggtttaac cggcatgatg
     9721 gcggcgtggc tgtttaatct tgatttgatt gaaggcttat taatcggcgc tatcgtcggc
     9781 tccaccgatg ctgcagcggt cttttctctg ctgggtggta aggggcttaa cgaacgtgtt
     9841 ggctcgacgc tggaaattga atccggcagt aatgatccaa tggcggtctt tctgacgatt
     9901 accctgattg cgatgatcca gcagcatgaa agcagtgtca gctggatgtt cgtggttgat
     9961 attctgcagc aatttggcct cgggattgtc atcggtcttg gcggtggtta tttactgctg
    10021 caaatgatta atcgcatcgc cctgcccgcc ggtttatatc cattgctggc attaagcggc
    10081 ggtattttaa tttttgcttt aaccacggcg ctggaaggta gcggtattct ggctgtttat
    10141 ctgtgcggtt ttctgctggg taatcggccg attcgcaacc gctacggcat cctgcaaaat
    10201 ttcgacggcc tcgcctggct ggcgcaaatc gccatgttcc tggtgctggg gctgttggtt
    10261 aacccaagcg atctgctgcc tattgccatt ccggcgctca ttttgtccgc atggatgatt
    10321 ttcttcgccc gtcctctttc agtatttgcc ggattgctac cgttccgtgg ttttaatctg
    10381 cgtgagcgcg tgtttatcag ctgggtggga ttacgcggcg cggtgccaat catcctggcc
    10441 gtgttcccga tgatggcggg gctggagaat gcgcgactgt tctttaatgt cgccttcttt
    10501 gtggttctga tttcactgct cttgcaggga acatcactct cgtgggcggc gaaaaaagcc
    10561 aaagtggtgg ttccgcccgt cggacgtccg gtgtcacgcg ttggcctgga tattcatccg
    10621 gaaaatccgt gggagcagtt tgtttatcaa ttgagtgccg ataaatggtg cgtgggcgcg
    10681 gcactgcgcg atttgcatat gccaaaagag acacgtattg cggcactgtt tcgtgataac
    10741 caactgcttc atcctaccgg cagcacccga ctgcgcgaag gcgatgtgtt gtgtgtaatt
    10801 ggtcgggaac gcgatctccc ggcgctcggt aaactgttca gccagtcgcc gccggtcgcg
    10861 ctggatcaac gcttctttgg tgacttcatt ctcgaagcca gcgccaaata tgctgatgtt
    10921 gcgctgatat atggcctgga agacgggcga gaatatcgtg ataaacagca aacgctgggt
    10981 gaaattgtcc agcagttgtt aggcgcggca ccggttgtcg gtgaccaggt agagtttgcc
    11041 ggaatgatct ggacggtggc cgagaaagaa gataatgaag tgttgaagat tggcgttcga
    11101 gtggctgaag aggaagctga atcttaaatt taatgaagca tctatcgccg gttacgatgc
    11161 ttttcttaac gcagcctgag cctgtatata aacttgtcat ttcctgattt tgatatgcac
    11221 attccaacat cagaagaagg ttaaattatg gatgacaaac cattgcaggc tctggccaat
    11281 aaactcaaaa caccggctca agaaagtcag cgtaatggct tcttccgact gcaacaggtg
    11341 aagaataacc agccccgcat taccggtatc cccgtcaacc atcaggggcc cctgcctgac
    11401 atcgcctgaa catattcccc cacgcaacct gccgcactga cgtagcccac gcaatacgcg
    11461 tttcagactg ctcagtatct gtagtggcgt ttcatccgcc tgtagcaccg gatgcatgag
    11521 cgatttctta tagttattcc tttgcttaat aacgattatt ttcgggtcgt tcgctctcgt
    11581 attttcttta atacgtcaga gctacctggg ccgtaataca gcatgtacca gacccgacac
    11641 ggcatttctg acctgggaaa aagtcaacga tgagtagtta tatcagcgca aatgacgtcc
    11701 tgtggctcct ttgcggtgac cacattcacg tctccggcgc tgccagagga tatttatttc
    11761 aaggatacgt agcattatgg ctagctttaa agacatctgc ccattcgccc ccgccattcc
    11821 cccatcttcc gttaacacag gcgtacgacg cccgccaggg cggctcttcc tgtcggtgga
    11881 actcaagaat caaattttgc agaacatgaa gtatctcagc ccgcaaaaat tcagctgggg
    11941 tgatgccccc tggcagatca tt
//
LOCUS       DAZUGK010000138        11739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZUGK010000138 DAZUGK010000000
VERSION     DAZUGK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.139"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..297)
                     /gene="yehE"
                     /locus_tag="ACHY5W_003318"
     CDS             complement(16..297)
                     /gene="yehE"
                     /locus_tag="ACHY5W_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFS4448169.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(560..1669)
                     /gene="apbC"
                     /locus_tag="ACHY5W_003319"
     CDS             complement(560..1669)
                     /gene="apbC"
                     /locus_tag="ACHY5W_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFS4448170.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1801..3834
                     /gene="metG"
                     /locus_tag="ACHY5W_003320"
     CDS             1801..3834
                     /gene="metG"
                     /locus_tag="ACHY5W_003320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4448171.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVGGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3975..7769
                     /locus_tag="ACHY5W_003321"
                     /pseudo
     CDS             3975..7769
                     /locus_tag="ACHY5W_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7779..11411
                     /gene="yehI"
                     /locus_tag="ACHY5W_003322"
                     /pseudo
     CDS             7779..11411
                     /gene="yehI"
                     /locus_tag="ACHY5W_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
     gene            11472..>11739
                     /gene="yehK"
                     /locus_tag="ACHY5W_003323"
     CDS             11472..>11739
                     /gene="yehK"
                     /locus_tag="ACHY5W_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFS4448172.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVI"
BASE COUNT         3049 a         3009 c         2902 g         2779 t
ORIGIN      
        1 aatcaggttg ccaaattaat cgtagcgatt taacacgatt cgacgtttgc tatcgctacc
       61 tgcaacaacc acttctgtcg ttacaccttt gacgggcgaa gaaaacagat gacgggtgtc
      121 aacattatac gttaactgac cacaatgctg ttgaggttga ccattaacca tagccatact
      181 gcatgttggg ggactgatcc tgccattaat cgctaatgtg gcagtctctg aagagaatgc
      241 gggagaagcc aatccatagg caagaaaaat aattcctgac agccaatact tattcattga
      301 acgttatccc tgtagtaaag gttatgcctg gatagaatga gtgcataaca aactatagct
      361 gtacatccac tacacagcca cgaaggatga taatgaagca ttgcctgtat aatcaatcga
      421 ctttgtagag tttcggacga aggtccgcag aatattcgca gtattaaata agtgttcatt
      481 aatgcggcaa aaactaatac accgcatcaa tgtaacatct ctgcggtgta tgaagaataa
      541 ccagaatggt taataggcgt tagaccgcgc ggaaggaaat ctcgcctgga atgacttcac
      601 cctgccagta gagctgagct gcaacgcggt cagccagctg gcggtagata gcggtaaatt
      661 cgctctccgg acggctgata accgttggcg tacctttatc gagatcctca cgtaaggaga
      721 tgtgtaacgg catctgcccc agcagctgtg tatggtattt ttcagccagt ttctctgcac
      781 caccggtacc gaaaatcggt tcatggtgac cacagttact gcaaatatgc acactcatgt
      841 tctcgacaat accgagcacc ggaacctcga ctttctcgaa catcacaatg ccttttttcg
      901 catcgatcag cgcgatgtcc tgcggggtgg tgaccacgac tgcaccggtt actggaatgt
      961 tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg catatcaagc accagatagt
     1021 cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc gccatcggtc
     1081 cacgccacac catcgcattg tcgtcagtga ccagataacc aattgaattg gttgccaggc
     1141 catgagacat aatcggtgcc atatgggtac cgtccggcga agttggacgc tgattttctg
     1201 cgcccaacat tgttgggatt gatgggccgt agatatcggc gtccagaatg ccgactttcg
     1261 ccccttctgc cgccagcgcc agtgccaggt ttaccgccgt ggacgattta cccacgccgc
     1321 ctttaccgga actcaccgcg ataatgttct tcacaccgtt aattcccggc tgattcttca
     1381 cgcgtttcag agtggctatg ttatgcgaca gtttccagtc gatagctttc gccccggtga
     1441 tgcgcagcag ttcggcgcta cattgctctt ttaactcttc gaacgcgcta tgccagacaa
     1501 acggcataac cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg tgcaacgctt
     1561 tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa attggccagt gtcccggcga
     1621 ccattgcgcg caaggcttct ggtgatttgg cctgggattg ttcgttcatc ccgactcctt
     1681 ttttgtatag ataaaccatc agctgatagt ttacctgaag aatatagaga agtacttact
     1741 taacattttc ccatttggta ctatctaacc ccttttcact attaagaagt aatgcctact
     1801 atgactcaag tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc taacggctca
     1861 atccacctcg gccatatgct ggagcacatc caggctgatg tctgggtccg ttaccagcga
     1921 atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg cccacggtac accgatcatg
     1981 ctgaaagctc agcagcttgg tatcaccccg gagcagatga ttggcgaaat gagtcaggag
     2041 catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc
     2101 gaagagaacc gccagttgtc agaacttatc tactctcgcc tgaaagaaaa cggttttatt
     2161 aaaaaccgca ccatctctca gctgtacgat ccggaaaaag gcatgttcct gccggaccgt
     2221 tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc aatacggcga taactgcgaa
     2281 gtctgcggcg cgacctacag cccgactgaa ctgatcgagc cgaaatcggt ggtttctggc
     2341 gctacgccgg taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa
     2401 atgttgcagg catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag
     2461 gagtggtttg aatctggcct gcaacagtgg gatatctccc gcgacgcccc ttacttcggt
     2521 tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc accgattggc
     2581 tacatgggtt ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag cttcgatgaa
     2641 tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac
     2701 ttccacagcc tgttctggcc tgccatgctg gaaggcagca acttccgcaa gccgtccaac
     2761 ctgtttgttc atggctatgt gacggtgaac ggcgcaaaga tgtccaagtc tcgcggcacc
     2821 tttattaaag ccagcacctg gctgaatcat tttgacgcag acagcctgcg ttactactac
     2881 actgcgaaac tctcttcgcg cattgatgat atcgatctca acctggaaga tttcgttcag
     2941 cgtgtgaatg ccgatatcgt taacaaagtg gttaacctgg cctcccgtaa tgcgggcttt
     3001 atcaacaagc gttttgacgg cgtgctggca agcgaactgg ctgacccgca gttgtacaaa
     3061 accttcactg atgccgctga agtgattggt gaagcgtggg aaagccgtga atttggtaaa
     3121 gccgtgcgcg aaatcatggc gctggctgat ctggctaacc gctatgtcga tgaacaggct
     3181 ccgtgggtgg tggcgaaaca ggaaggccgc gatgccgacc tgcaggcaat ttgctcaatg
     3241 ggcatcaacc tgttccgcgt gctgatgact tacctgaagc cggtactgcc gaaactgacc
     3301 gagcgtgcag aagcattcct caatacggaa ctgacctggg atggtatcca gcaaccgctg
     3361 ctgggccaca aagtgaatcc gttcaaggcg ctgtataacc gcatcgatat gaggcaggtt
     3421 gaagcactgg tggaagcctc taaagaagaa gtaaaagccg ctgccgcgcc ggtaactggc
     3481 ccgctggcag atgatccgat tcaggaaacc atcacctttg acgacttcgc taaagttgac
     3541 ctgcgcgtgg cgctgattga aaacgcagag tttgttggag gttctgacaa actgctgcgc
     3601 ctgacgctgg atctcggcgg tgaaaaacgc aatgtcttct ccggtattcg ttctgcttac
     3661 ccggatccgc aggcactgat tggtcgtcac accattatgg tggctaacct ggcaccacgt
     3721 aaaatgcgct tcggtatctc tgaaggcatg gtgatggctg ccggtcctgg cgggaaagat
     3781 attttcctgc taagcccgga tgccggtgct aaaccgggtc atcaggtgaa ataatccccc
     3841 ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt cctaaagttg ttttcttgcg
     3901 attttgtctc tctctaaccc gcataaatac tggtagcatc tgcattcaac tggataaaat
     3961 tacagggatg cagaatgaga cactttatct atcaggacga aaaatcacat aaattcaggg
     4021 cagttgagca acagggaaac gagttgcata tcagttgggg aaaagttggc accaaagggc
     4081 aaagccagat aaaaagtttt tcagatgctg cggcagcggc aaaagcggag cttaagctga
     4141 ttgcggagaa ggtgaagaag gggtatgtgg agcaagcgaa ggataattct ttgcaacctt
     4201 cccaaacggt aacgggctct ctcaaggtag cggacttatc caccattatt caggaacaac
     4261 cctctttcgt agcagaaacc cgtgcgcctg acaaaaatac agatgctgtt ttaccgtggc
     4321 tggcgaaaga tattgctgtc gtttttccgc ccgaagttgt acacaccacg ttaagtcatc
     4381 gccgctttcc cggagttcct gttcagcaag cagacaaatt gccccaatta cgtcgcttag
     4441 cctgtagtgt gtcgcaacgg gataataaaa cagccacatt tgacttcagc gcctgttctt
     4501 tagaatggca aaacaccgtc gcccaggcga tcagtcagat cgacggcctg aaaacaacac
     4561 agttaccatc accagtaatg gctgtactca cggcacttga aatgaaatgc acaagatata
     4621 aagtgcgtga ggatgttatg gatcagatcg tccaggaagg cggtctggaa tatgctactg
     4681 atgtaataat acaccttcaa cagattgata ttgaatggga ttatgcgaat aatgtcatta
     4741 ttattctgcc gtctggcatt gcacctagct acttggagca atattccaga tttgaatgac
     4801 gcctacgtaa acatttatca ctgacggaag agtctctctg gcaaaaatgt gcacaaaaac
     4861 ttattgccgc aattccacat attccagaat ggcggcaacc attaattgct ttgttattac
     4921 ccgaaaaacc agaaattgca catgaaattg cccagcgtct actggggcaa aaaaaattac
     4981 cctcgcttga gtggttaaaa atagtggcga ctgatgagca cattcttgcc tcattagaaa
     5041 aatatcacga accatatgcc atttttgatg attactattg tggtgcgata tggtcagcca
     5101 ccgtattaca ggagcaaggt gttgcagccc tgccccgatt tgctccctat gccgcaagtg
     5161 actactgcgc cgatgtgttg cgtcatatca atcatccgtt cgcattgaca ctgcttatac
     5221 gtgtagccgg gcaaactaaa cgctgtcacg atcggatgac gaaagccatt gctgcgttcc
     5281 cacatgcagc aatggcggca ctgacggaac ttcttgggca aaaagaagag aacagttggc
     5341 gcattatgct aatgacaatg cttatctcac aaccagcact ggcagaacag gtcattccct
     5401 ggctctcgac acccgcagtt gccgtactga aatcatgcca gcaacaactg acacagccct
     5461 caaaccatgc cagcgccgat ctactgccag ccgtagtagt ctcccctccc tggctttcga
     5521 aaaagaaaaa atcgccgatt ccggtgctgg atttagcgcc attaggcatt gagccaatct
     5581 gttatctgac agaagaaatc agtaatcaac ttttggcgaa atatatctgg tattcaaaac
     5641 acatcacggt tagccatgaa gaaagtacta ccaacctgtt ggcaaggatg ggttttcaac
     5701 gacggatcgc tggtacatat attaaagctc ccgaagcggt agttgaggca tggctaaatg
     5761 aagattattc aaccttacta agtgaattta aggtgtttca ttcacctacc gggcattatt
     5821 ggcagttggg gattttgaca acattgccgc tggagaaagc agtaaaagca tggaatgccc
     5881 ttaccctatc tccacatacc gataccgaat actccatgtt acattttgga ctcaaagggt
     5941 tacctgggtt agtaaactca cttgcacgct atccacaaga agccttgccc atcacgaatt
     6001 acttcgcagc gagtgagctg gctcctgccg tcgcccgtgc cttcaacaaa ctgaaaacgc
     6061 tacgcgaaaa cgcccgtagc tggctgttga aatacccgga acatgccctt accggcctgc
     6121 tgcctgcggc gctcggcaaa gccggtgaag cacaggataa cgcccgcgct gccttgcgta
     6181 tgcttaccga aaacggtcat cagccattac tgcaagaaat cgcccgacgt tataaccagc
     6241 cggaagtaac cgatgcggtg aacgctctgc ttgcgctcga tcccttagat aatcacccga
     6301 caaaaatccc cactcttccg gccttttatc agccatcgct ctggacgcgc ccggtattaa
     6361 aagcaaatgc ccaatcactg ccagatagcg ccctcctcca cctcggtgaa atgctccgct
     6421 tccctcagga agaggctctg tatccgggat tattgcaggt gaaagacgtc tgttccgccg
     6481 actcactggc gggatttgcc tgggatctgt ttaccgcctg gcagaccgct ggcgcgccgt
     6541 cgaaagagag ttgggcgttc actgcgttag gcgttctcgg taacgatgac accgcccgca
     6601 aactgacgcc attaatacgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg
     6661 ggttggatat tctcgctgct atcggtagtg atatcgccct tatgcagctt aacggcatcg
     6721 cccagaaact gaaattcaaa gcattacagg agcgggcaaa agaaaaaatt gccgacattg
     6781 ccgagagccg cgaactcacg gtggcggagc ttgaagatcg gttagcaccg gatctcggtc
     6841 tggatgataa cggttcgctg ctgctggatt ttggcccacg gcagttcacc gtcagctttg
     6901 atgaaacctt aaaaccgttt gtgcgtgatg tttccggcag ccgcctgaaa gacctgccca
     6961 aaccgaacaa aagcgatgat gaaacgcggg cgaacgatgc ggttaaccgc tacaaattgc
     7021 tgaaaaaaga tgcgcgtacc atcgccgccc agcaggtagc aaggctggaa tccgccatgt
     7081 gcctgcgccg ccgctggtcg ctggaaaact tccagttctt cctggttgag catccgctgg
     7141 ttcgtcactt aacccgccgt ctgatttggg gcgtttatag cgccgaaaac cagctactgg
     7201 cttgctttcg cgtagcagaa gataacagct ccagcaccgc tgacgatgat cttttcaccc
     7261 tgccggaagg cgatatctct atcggcactc ctcacgttct ggaaatatca ccaacggatg
     7321 ctgccgcctt tggtcagctt tttgccgact acgaactgct accaccgttc cgccagctcg
     7381 accgtaacag ctacgccctg acagaagccg agcgcaatgc cagtgaactg acccgctggg
     7441 caggcagaaa atgcccgagt ggtcgggtca tggggctggc gaataaaggc tggataaagg
     7501 gcgaaccaca ggatggaggc tggatcggat ggatgatcaa acctttgggt cgctggtcgt
     7561 taatcatgga aatcgatgaa ggctttgcgg taggcatgtc gccagccgaa ctcagcgctg
     7621 agcagctctt aagcaagctg tggctatggg aaggcaaagc agaaagatat ggctggggga
     7681 gtaattcaac acaggaagcg cagttctccg taatcgatgc catcaccgcc agcgagctaa
     7741 ttaacgatat tgaagccctg tttgaataag gaaagagcat ggacaaggaa ttaccgtggc
     7801 tggcggataa cgcccaactg gaactgaaat ataaaaaagg caaaacgccg ctcagtcatc
     7861 gtcgctggcc gggcgaacca gtgtccgtta tcactggaag tctcatccag acattgggtg
     7921 atgaattgct acaaaaagct gagaagaaaa aaaacattgt ctggcgttat gagaattttt
     7981 cactggagtg gcagtccgcc atcacgcagg ccatcaactt gatcggcgaa cacaaaccct
     8041 caatcccggc ccggacaatg gcggcgctag cctgtatcgc gcaaaatgac agccaacagt
     8101 tgctcgacga aatcgtccaa caagaggggc tggaatatgc gactgaggtg gtgattgcac
     8161 gccagtttat tgcgcggtgt tatgagagtg atcctctggt agtgacattg cagtatcagg
     8221 acgaggatta tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgactgc
     8281 gtaagcatct ctctctggca gaggaaagct gctggcagcg ttgcgccgac aaactcattg
     8341 ccgcactacc aggaataacc aaagttcgcc gcccttttat tgcgctgatc ctcccggaaa
     8401 aaccagaaat agccaatgag ttggtaggcc ttgaatgccc gcgaactcat tttcattcta
     8461 aggagtggtt aaaagttgtt gctaatgacc ccacagcggt gagaaaactc gaacactact
     8521 ggagccagga tatatttagc gatcgagaag ccagctacat gtcgcatgaa aaccacttcg
     8581 gctacgcggc ctgcgccgcc cttttgcgcg aacaaggact ggcagccatt ccgcgcctcg
     8641 cgatgtatgc ccataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag
     8701 tcatccgcac cttgctactg gtggctgata aaaacaaacc cagcctgcaa cgtgtagcta
     8761 aataccataa aaacttcccc catgcgacgc tcgccgcact ggcagaactg ctggcgttaa
     8821 aagaaccacc agcccgccca ggtaatccaa tcatcgaaga caaaaagctg cctgcacagc
     8881 aaaaagcacg cgatgaatac tggcgtacgc tgttacaaac gctgatggca tcgcagccac
     8941 aactggcaga agaggtgatg cagtggttaa gtactcaacc ccagtcagtg ctgaagagtt
     9001 atttatcggc accgcccaaa ccggttattg atggcaccga taacagcaat ctgccagaaa
     9061 tcctcgtttc accaccgtgg cgtagtaaga aaaaaatgac agctccacgt cttgatttgg
     9121 caccgctcga attaactccg caagtttact ggcaaccagg cgaacaagag aggcttgccg
     9181 ccactgagcc tgcccgttat ttcagcacgg aatctcttgc gcaacgcatg gaacaaaaaa
     9241 gtggacgagt tgtattacag gaactgggtt ttggggatga tgtatggctg tttctgaatt
     9301 atatactccc cggaaaactg gatgctgcac gcaattcact cattgttcag tggcattact
     9361 accaggggcg ggttgaagag atcctgaatg gctggaactc cccggaagca caattagcag
     9421 aacaggcgct ccgcagcggt cacatagaag cgttaattaa catatgggaa aatgacaact
     9481 actcacgtta tcgtccggaa aagagtgtct ggaacctgta tttattggca cagttgccgc
     9541 gtgagatggc tttgaccttc tggctgcgta tcaatgagaa aaagcatctg ttcgcgggtg
     9601 aggactattt tctcagtatc ctcggattgg atgcgctacc aggtctgctg ttggcttttt
     9661 cacatcgtcc aaaagaaaca tttccgttaa ttttaaattt cggcgcaaca gaactggccc
     9721 tgcccgttgc ccgcgtctgg caccgttttg cgggccagcg taatctggct cgccagtgga
     9781 ttttacaatg gccggaacat acggctactg cacttattcc actcgtcttt gttaaaccct
     9841 gcgacaacag cgaagcggca ttatttgccc ttcgtttact gtatgaacaa ggacatagtg
     9901 aattactgca aacggttgca aaccgctggg atcgcgctga tatgtggcca gccctggaaa
     9961 aaatacttac ccagaacccg atggaaattt acccggcacg cattccaaaa gcccctgatt
    10021 tctggcatcc gcaaatgtgg tccaggccgc gccttatcac taataatcaa actgttacca
    10081 atgacgctct ggaaattatc ggcgaaatgc tgcgctttac ccagggggga cgtttttata
    10141 gcgggctgga acaactgaaa acgttctgcc agccacaaac gctggcagct tttgcttggg
    10201 atctcttcac tgcgtggcaa caagctggtg cccccgcaaa agacaactgg gcatttctgg
    10261 cgttaagtct ctttggtgac gaaagcacgg cacgggatct gacgacacag atcctcgcct
    10321 ggccacaaga aggcaaatct gcccgtgctg tcagcggcct gaacatcctt accctgatga
    10381 ataatgatat ggcgctgata cagctgcatc atatatcgca acgggctaaa tcccgcccct
    10441 tacgtgataa cgcggcggaa tttcttcagg tggtcgcaga aaatcgcggg ctaagccagg
    10501 aagagctagc ggacagatta gtcccaaccc tgggccttga tgatccgcag gcgttgagtt
    10561 ttgattttgg tccccggcag tttaccgttc gcttcgatga aaatctcaac ccggttatct
    10621 ttgatcagca aaacgttcgc cagaaaagcg ttccccgttt gcgcgccgat gacgatcaac
    10681 tgaaagcgcc cgaggcactg gcccgactaa aagggctaaa aaaagatgct actcaggtga
    10741 gcaaaaacct gctcccgcgt cttgaaacgg ccctacgtac cacccgacgc tggtcgctgg
    10801 cagattttca ttctctgttt gttaatcatc cctttacccg tctggttacc cagcgattaa
    10861 tatggggggt ttatccggca aatgaaccgc gttgtttact caaagccttt cgtgtggccg
    10921 cagaggggga gttctgcaat gcgcaagatg agccaattga cctgcctgcg gacgctctga
    10981 ttggcattgc ccacccgtta gaaatgacag cataaatgcg cagtgaattt gcacagcttt
    11041 ttgccgatta cgaaattatg ccgccttttc gccagttgtc gcgccgcacg gtgctgctca
    11101 cacctgacga gtcaaccagt aacagcctga ctcgctggga aggtaaatcc gctaccgttg
    11161 ggcaacttat gggaatgcga tacaaaggct gggagtcagg ctatgaggac gcatttgtct
    11221 ataacctggg tgagtaccgg ctggtcctta agttttcacc cggttttaac cactacaatg
    11281 ttgatagcaa agcgctaatg agcttccgtt ctcttcgagt gtaccgtgac aataaatccg
    11341 tcacttttgc cgaacttgat gtgtttgatt tgagtgaggc gttaagcgca cctgacgtca
    11401 ttttccatta aaacacagcg ggcagtgatg caactgcccg ttatcaacac gacttaccag
    11461 cggggaaagc gatgatcgtg caaaaagagc tggttgctat ttacgattat gaggtccctg
    11521 tacctgaaga tccgttttcc ttcagacttg agatccataa atgctctgaa ttatttacag
    11581 gttccgtcta tcgactggag cgattccggc tacgtccaac atttcatcaa cgtgatcgag
    11641 aagatgctga cccgctaata aatgatgcgt tgatttatat aagagatgag tgtattgatg
    11701 agcggaaatt acgaggtgaa tcacctgaaa ctgtaatag
//
LOCUS       DAZUGK010000139        11722 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZUGK010000139 DAZUGK010000000
VERSION     DAZUGK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.140"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..762
                     /locus_tag="ACHY5W_003324"
     CDS             82..762
                     /locus_tag="ACHY5W_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448173.1"
                     /translation="MRAKPTLTFNGFSMHPLDALKNTAVLFEAGYLLATSNNHEYCEI
                     GDTIVALATDYAFEVKNAIFYSRQDIAPEKQPEYMVNLSTQREACGLTTTELARLLDL
                     DEEIILQWESGEYEPTISMLIPMANVLGCDPLSLLSEKNSAAAVTVNQPDIQEESIGT
                     RIEAARKKVGLTEADLARMIHTYNDPINDWECGICEVPASQIIPLANALGCDPMWLLT
                     GGPIARAS"
     gene            1143..1349
                     /locus_tag="ACHY5W_003325"
     CDS             1143..1349
                     /locus_tag="ACHY5W_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448174.1"
                     /translation="MTETTSQQDNYEGICLEPDSFAVNVYHLLHATQVLHMSSNHETK
                     VLGSEILNFACEYAKAAAEKELAQ"
     gene            1546..1764
                     /locus_tag="ACHY5W_003326"
     CDS             1546..1764
                     /locus_tag="ACHY5W_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448175.1"
                     /translation="METSARNKMQNEPEQGGVIHEKVRVLLTIENGKVIYSKHLLDNE
                     FVGCMDTFLWMAKRAGYTIIPPAKEQTL"
     gene            1761..2342
                     /locus_tag="ACHY5W_003327"
     CDS             1761..2342
                     /locus_tag="ACHY5W_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448176.1"
                     /translation="MNHSEFRPEVTPHGIKIGNTTIDYVEAVQRLNDGEYDYPNSHGL
                     RIMQCIAEADDAGLLGRFSVDMKIAQWRWLYVTTFINEEEGKNGTIDIPNDNGTTDRA
                     VIYKGKHGCLSIYPGPLRIALQNHVEWRFIEKYGEAEGMGRVLFLYQKMLIADPDNGF
                     IVSAMGREGLELLLDEMIHDLNTHGMPETPLEH"
     gene            2345..2626
                     /locus_tag="ACHY5W_003328"
     CDS             2345..2626
                     /locus_tag="ACHY5W_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448177.1"
                     /translation="MSNQNKINVFQVESKHKTPVIKHVRRHTLIYTPEEFMAMPMIKK
                     FIRDNPDHIAIDKHNGEIMLSRELAEIYCNVNNGKKLKKAIRKKSGVTK"
     gene            2623..2991
                     /locus_tag="ACHY5W_003329"
     CDS             2623..2991
                     /locus_tag="ACHY5W_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4448178.1"
                     /translation="MNSIETQCSSSEVMNYDPNLTLYGRMAKQTVLLTFGLWEYRETF
                     EVSVGGNLTGLDVISCAIESLYATLPYEEVEDERDIIATINIGGMECKDENLNGELWL
                     AGMLISAEIISIEPATNIRL"
     gene            3023..3265
                     /locus_tag="ACHY5W_003330"
     CDS             3023..3265
                     /locus_tag="ACHY5W_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4448179.1"
                     /translation="MFALINQGQLYTDSAGYPIKIIRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRKILAGTEQEMHLKKLRAMQRR"
     gene            3269..3403
                     /locus_tag="ACHY5W_003331"
     CDS             3269..3403
                     /locus_tag="ACHY5W_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448180.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            3422..3676
                     /gene="xisR"
                     /locus_tag="ACHY5W_003332"
     CDS             3422..3676
                     /gene="xisR"
                     /locus_tag="ACHY5W_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS4448181.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3710..4996
                     /locus_tag="ACHY5W_003333"
     CDS             3710..4996
                     /locus_tag="ACHY5W_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4448182.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            4980..5777
                     /gene="yecE"
                     /locus_tag="ACHY5W_003334"
     CDS             4980..5777
                     /gene="yecE"
                     /locus_tag="ACHY5W_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4448183.1"
                     /translation="MEPDSEIISLNISCFNGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5830..6225
                     /gene="yecN"
                     /locus_tag="ACHY5W_003335"
     CDS             5830..6225
                     /gene="yecN"
                     /locus_tag="ACHY5W_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFS4448184.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6266..7009
                     /gene="cmoA"
                     /locus_tag="ACHY5W_003336"
     CDS             6266..7009
                     /gene="cmoA"
                     /locus_tag="ACHY5W_003336"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFS4448185.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPSTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7006..7977
                     /gene="cmoB"
                     /locus_tag="ACHY5W_003337"
     CDS             7006..7977
                     /gene="cmoB"
                     /locus_tag="ACHY5W_003337"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFS4448186.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSTLALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDSHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8142..10571)
                     /gene="torZ"
                     /locus_tag="ACHY5W_003338"
     CDS             complement(8142..10571)
                     /gene="torZ"
                     /locus_tag="ACHY5W_003338"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFS4448187.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     VAMNVEVNDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNVTWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10596..11696)
                     /gene="torY"
                     /locus_tag="ACHY5W_003339"
     CDS             complement(10596..11696)
                     /gene="torY"
                     /locus_tag="ACHY5W_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFS4448188.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         3112 a         2799 c         2752 g         3059 t
ORIGIN      
        1 ccaaaaaaat agaacttgct attgccataa tttatagcag gttctattat gctctcattc
       61 caaatagatg gagttaatga gatgagggca aaaccgactc tgacttttaa tggcttttcc
      121 atgcacccac tggatgcgct aaaaaatacc gccgttcttt ttgaagctgg atatttatta
      181 gctacatcaa ataaccatga atactgtgaa attggcgata ccatagttgc tttggccact
      241 gattacgcct tcgaagtaaa aaacgcaatt ttttattcaa gacaagatat tgctcctgaa
      301 aaacagcctg aatacatggt taaccttagc acccaacgtg aagcctgcgg tttgacgacc
      361 accgaactgg ccagactgct cgatcttgac gaagagatta tcctgcaatg ggagagtgga
      421 gaatacgaac caaccatcag catgctaatc cccatggcaa atgtcctggg atgcgatcct
      481 ctttctctgc tgagtgaaaa aaatagcgct gctgctgtta ccgtaaatca gccagacatc
      541 caggaggaaa gcattggcac acgtatagaa gccgcacgta aaaaagttgg actgacagaa
      601 gcagacctgg cacgcatgat tcacacctac aacgacccca taaacgactg ggagtgcggt
      661 atctgcgaag tccccgcaag ccagatcatt ccactggcta atgcgcttgg ttgcgatccg
      721 atgtggctgt taactggtgg gcctattgcc cgggcttcat aagataccat ggggcaataa
      781 catcgccgcg cttttctaca agatgagagc gaatttcacg aagttcttca actgaggagc
      841 cgaaagccag agtgacataa tcgccacttc tgccatcaag atacatacgg acatttctct
      901 caaccattgc ggaaacagtc tcaatatgaa aacacttctg agactcacta tataacagaa
      961 catacatgtc agctgaggaa gccatgaaaa agttcgaaaa cataactgtt ctccatgttg
     1021 ataactttgg ttatacaaac caggaacttc tcccggaggt tgtaaaggca atagatgttg
     1081 ccgatatagt gattagagga aagagaattg tcaaaaacag gctcgcatgc acttcaggag
     1141 caatgacaga aacaacctca cagcaagata attacgaagg catttgtctg gagcctgatt
     1201 catttgcggt aaatgtttat catttattgc atgcaacaca ggtattacat atgtccagta
     1261 atcacgaaac aaaagtactt ggcagcgaaa ttctgaattt tgcatgtgaa tatgcaaaag
     1321 ctgctgccga aaaagaatta gcgcaataac aataaatatt ccctgaatgt ttattacggt
     1381 tttatcgccg gggattgttg caacctttat tcgcaggaga ttatgttatg accttcctga
     1441 aacataaggc atcgtataaa actgcctgcc tcattgcaca acatggagat tcttatcttc
     1501 atatagccaa cctgtatttg cgcaaggctt atgggagata aaataatgga gacatcagca
     1561 cgaaataaaa tgcagaatga gcctgagcag gggggggtaa tacacgaaaa agtaagagtg
     1621 ttgctaacca ttgaaaatgg gaaagtaatt tactcaaaac atctgttgga taatgaattc
     1681 gttggctgca tggatacatt tctgtggatg gcaaaaagag cgggttacac gattattcca
     1741 ccagcaaagg agcaaacatt atgaatcatt cagagttccg accagaagtt acgccacatg
     1801 gcataaaaat tggcaataca accattgatt atgttgaggc cgtacagcgg cttaatgatg
     1861 gtgaatacga ttatccaaat tctcacggtt taagaattat gcaatgtatt gctgaagccg
     1921 atgatgccgg attactggga cgattttcag tcgatatgaa gattgctcaa tggcgatggc
     1981 tgtatgtgac gacgtttata aatgaagaag aaggcaagaa cggcaccatt gatatcccta
     2041 acgataatgg aactacagat cgcgcagtta tttacaaggg taaacatggt tgcctgagta
     2101 tctacccagg gccacttcgc attgccctgc aaaaccatgt cgaatggaga ttcattgaaa
     2161 aatatggcga agctgaaggc atggggcgag ttctgtttct ctatcaaaaa atgctcatcg
     2221 cagatcctga taatggcttc attgtctctg ctatgggacg agaagggctt gaacttcttc
     2281 tggatgaaat gattcacgat ctgaatactc atggtatgcc agaaacacca ttggaacatt
     2341 aaacatgtca aatcagaata aaattaatgt atttcaggtt gaatcaaaac ataaaacacc
     2401 tgtgataaaa cacgttcgcc gccatacact catctatacg ccagaagagt ttatggcaat
     2461 gccaatgata aagaagttta ttcgtgataa tcccgatcac attgctatag ataaacataa
     2521 cggagaaata atgttatcac gcgaacttgc cgaaatttac tgtaacgtga ataacggtaa
     2581 aaaattgaaa aaggcaatcc gaaaaaaatc aggagtaaca aaatgaatag catcgagaca
     2641 caatgttcct catctgaagt tatgaactat gacccgaatc tgacgttgta cggacgcatg
     2701 gcaaaacaaa ctgttctatt aactttcggg ctatgggaat accgcgaaac attcgaagtt
     2761 tctgtcggcg gcaatctgac cggactggat gttatcagtt gcgccattga aagcctgtac
     2821 gcaacgctgc cttatgaaga agtcgaggat gagcgcgata tcatagccac cattaatatc
     2881 ggcggcatgg aatgcaagga tgaaaacctg aacggagaac tctggcttgc cgggatgctt
     2941 atctcggcag aaatcatcag tattgaaccc gctacaaaca tacgactctg aagttctcac
     3001 aactcaggga gcaggagaaa aaatgttcgc tttgattaat caggggcaac tgtataccga
     3061 tagtgctggc tacccgataa aaattattcg ctgcataaac aacactgtgt tgtacagaag
     3121 aatggatggg cgaacacagt cggtaaaaat aaacgatttt aatgaactgt ttgaacgaat
     3181 cgatcaccag gagtaccgca aaattctggc gggcactgag caggaaatgc acctgaaaaa
     3241 attacgcgca atgcaacgga ggtgatacat gcatacggct tttgagttct gggttcgcaa
     3301 gacattcggc aatcgctacg acctgacccg tgatgtcgac ggcttctact gccgtgaagt
     3361 tgtgaaacga atgtttgacg tgtggtgcca ctgccgtgga tgaaagtttt atgaggttgg
     3421 catgcagaca atcatctatc agataacccc cagcaaatgg tgtacggaga gagtccttat
     3481 tgcatcaaca gggctaaagc ccggcaccat cgagcgggct agaagaaagt catggatgca
     3541 gggaaaagaa taccgccatt acgctgtaga aggtgatcct gggcactaca gtgaatgcct
     3601 gtacaacatc gaagaaatta tgcgatggat cgaaaaccag aaacaaccag gtgccaaaaa
     3661 tgcaagttcc ggttaacctg ttaatgctcc tggacgtctg ggaggtttaa tgagtaacgc
     3721 atcatacccg acaggcgttg aaaaccatgg aggatcactc cgtatatggt ttcactataa
     3781 tggcaaacgt gtcagagaaa acctcggtgt tcctgacacc gccaaaaacc ggaagatcgc
     3841 aggtgaactt cgcacttccg tttgttttgc aatcagaatg gggagtttcg actacgccgc
     3901 gcagttccct aattccccta acctgaaaca ctttggtctg ggaaaaagag agataaccgt
     3961 taaggcactt tcggaaaaat ggttggacct taagaaaatt gagataggaa gtaatgcatt
     4021 cagtcggtat caatccgtgg tgagaaacat gcttcctcgc ataggggaaa aacgtcttgc
     4081 ttcgtcggta acaaaggaag atttactgtt tatcaggaaa gatttgttaa ccgggtatca
     4141 taatctctct aacggaaaaa caacgccgat taaagggagg tcagtagtta cggttaatta
     4201 ctacatgacg acaattgcag gaatgtttca atttgcggct gataacggct atatcgtgtc
     4261 aaacccattt aacggcctga caccattaaa gagatccaga acagaaccag atccgctcac
     4321 acgagacgaa tttattcgtt ttattgatgc ctgtcaccat caacaaacga aaaacctgtg
     4381 gtccttagca gtatacacag gcattcgtca cggtgagcta atatctctcg cttgggagga
     4441 tattgattta aaagctaaaa caatgactat ccgtcgtaat tatacaaaac tcggggagtt
     4501 cactctacca aaaacagaag cgggaactga tcgtgttatt catcttgttc aaccagctgt
     4561 tgatgccctg aaaagccagg ctgaaatgac aagacttggc cctcagtatc aaattgacgt
     4621 caagcttcgg gagttcggtc gcactgcacg ccatgaatgc acgtttgttt ttaatccgca
     4681 actggtgaaa aaatgccagc aagtcggtca ccactataaa gcagattcca tcagagattc
     4741 ctgggcatct gcattaaggc gagcaggact gcggcacaga aaagcctatc agtccaggca
     4801 tacttatgcc tgttgggcat tatcggcagg agcgaatcca agcttcatag caaaccaaat
     4861 gggccatgca aatgcacaaa tggtattcaa cgtttacgga gcatggatga aagataacaa
     4921 tatcgggcaa atagaactac tcaataagca gttgacggag agtgtcccat acatgcccca
     4981 tagagccaga ctctgaaata atatctttaa atatcagttg ttttaatggt agttgccgtc
     5041 acattaactg cgtggagggc aacaccacgc tttacgccct gccgaaaccc gaggttgtcc
     5101 tgcgctggcg tgaacagacc acagatgact tccgcttctg ttttaagttt ccggcgacca
     5161 tttcgcatca ggcagcatta cgacattgcg atgatttagt gactgaattt ttgacccgca
     5221 tgtcaccgtt ggctccgcgc attgggcaat actggctgca actgcctgcc acattcggcc
     5281 cacgggagct gcctgcgctt tggcattttc tcgattctct ccctggcgaa tttaattatg
     5341 gtgtggaagt ccgccatcca cagtttttcg ccaaagggga agaggaacaa acgcttaatc
     5401 gcggtttaca tcagcgcggc gttaatcggg tgattttaga cagccgcccg gttcatgcag
     5461 cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac
     5521 atgctgtact gacggcgaca aatccgctga tccgttttat cggtagtgat gatatgacgc
     5581 aaaaccggga attatttcag gtctggttac agaaattagc gcagtggcat cagaccacta
     5641 cgccttatct ttttttacat acgccagaca ttgcccaggc cccggaactg gtacataccc
     5701 tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct attccacagc
     5761 aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc cattttaaag
     5821 ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg cgttgttatt aatgaagttc
     5881 tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggcga cggcggtttt
     5941 agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg
     6001 attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc
     6061 ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca ccgtctgttt
     6121 cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg
     6181 aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac aatacgccac
     6241 tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt ctgcccctat
     6301 cgccagactg ggtgactgga cctttgatga acgggtagct gaagtcttcc cggatatgat
     6361 ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt tagccgagcg
     6421 cttcgttcaa cctagtacgc aggtttacga tctgggttgt tctctgggcg cggcgacgct
     6481 ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg acaactcccc
     6541 ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta cgccagtaga
     6601 cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa
     6661 ttttaccctg caattcctgg aaccttccga gcgccaggcg ttactggata aaatttatca
     6721 agggctaaac cccggcggcg cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa
     6781 agttggtgaa ctgttgttca acatgcacca cgactttaaa cgtgccaacg gttacagcga
     6841 actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg attccgtgga
     6901 aacacataaa gcacgcctgc ataacgccgg ttttgagcat agtgagctgt ggttccagtg
     6961 ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat cgactttggt
     7021 aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac gctgcccgcg
     7081 cagattgcta actggcagcg cgagcagcag cacgggttgt ttaagcagtg gtcaaatgcg
     7141 gtggagtttc tacctgaaat taaaccgtat cgtcttgatt tattgcatag cgttacggca
     7201 gaaagcgaag agccactgag cgcagggcaa attaaacgca ttgaaacgct gatgcgcaac
     7261 ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga taccgaatgg
     7321 cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac cggacgcacc
     7381 attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg cgcaggcgcg
     7441 cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt
     7501 aaactactgg gtaatgatca gcgcgcgcat ttgttaccgt taggtattga acaacttccg
     7561 gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttatcatcg tcgttcaccg
     7621 ctggagcatc tctggcagtt gaaagatcaa ctggtcaatg aaggtgaact ggtgcttgaa
     7681 acgctggtta ttgatggtga tgagaacacg gtgctggtgc cgggcgatcg ttacgcgcaa
     7741 atgcgtaatg tctatttcat cccttccacg ctggcgctaa aaaactggct gaagaagtgt
     7801 ggttttgttg atattcgcat tgctgatgtg agcgttacca ccacagaaga gcagcgtcgt
     7861 accgagtgga tggtcaccga atcgctggca gattttcttg attcacatga tccgggtaaa
     7921 acggttgaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg
     7981 tctggtatta ctgccggatg cggcgtgaac gccttatccg gcctacaaaa ccttgctaat
     8041 tcaatatatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc agctttacgt
     8101 ttgcataacc tcagcgcccg tttccgggcg ctattcactt cttacgcctg aacagctggc
     8161 tgatcaaacg ccgttaactt cggtgcgtta cccgtatatt tttcgatata caccagcgca
     8221 gagttaccgg cacaggcatt tgccagctgc gagctgggga tatccgccgt taacacgttc
     8281 gcactgccgt ttttacacaa gccattttcc agatctggcc atgcaccttc atgcacgcat
     8341 accacgcctt ttttgatccc gtcagtcacc acagcgcctg tcaggatctg accacgttta
     8401 ttccagacac ggaccagatc gccattcgca ataccaaagc gagcagcatc ttcggtgtga
     8461 atagtgattg gttcacgatc ggcaaccgca tattttttac gcagttgcgc atagttaagc
     8521 tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg
     8581 gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc cttatagcca
     8641 aatttttcca gcgttttgga gtaaatctca attttgccgc ttggcgtacc cagcgcattt
     8701 ttcaccggat cggcgcggaa atcaccataa cgaacgtact gttcgttctt ctcgctgcgg
     8761 cgcatttcga tcagtttatt ttgctgccag aaggcattaa acattggcat agtgacgcgt
     8821 tgcgcacggg cacctttctg agcggcatca tagaaaaatt tcagccacgc catttcatct
     8881 ttaccttcgg tatagatctc ttttccgcca ggtttcagta attccgcaag ttcggcaaac
     8941 acgtcaaaat cgtttcgcgc ttcaaattgc ggagcgacag cctgcttcat cggtacaata
     9001 tgctggttgc tgtaatcacc agtcatcgtc aagtcattgc gctcaaacga tgtggtgatc
     9061 ggtaatacga tatctgcatg tttagcagct gcggtccagt agcattcaga aacgacgatc
     9121 atctccggtt tctgccatgc tttgatcaga cgattagtat cctggtgatg ggtaaagtta
     9181 ccgccacccg cccaccagat cattttgata ttcggatacg tttgttcttt accgttatgc
     9241 tgatatttgc caccaggatt ttccagcgca tcgacgatac gggcaacagg aatcgccgtc
     9301 attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga catttccggc
     9361 agcacgccgc caacacgcgt cgggttgcca ccgttggagt aatgataaga gaaaccaaaa
     9421 ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag catccagtgt
     9481 ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat tgtgcgattc
     9541 gccgccatca gttctgcgag tttgactatc tgcgcttccg gtacgccagt aatttccgcc
     9601 gcccaggccg cgctcttcgg agtattatcg ctcttacctg tcagatactc ttcaaactgc
     9661 ggatacccgg tagtgtattt ttcaaggaac actttatcgt gtttgccttg tgtcatcagg
     9721 gtatgcgcaa tccctaacat caatgccacg tcggtgccca tattcggcgc gatccaggtg
     9781 acattatcgc caaagaattc gatggtttcg gagcggatag gatcaatggc aatcactggt
     9841 ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt actgctccag
     9901 gcaattttta aggtattcag cgggttcatt ccccacagca ccacaacctg gctgttttcc
     9961 agaatcagcg gccaggaggt ctgctgttca tacacctcta cagaaccgac cacatgcggc
    10021 atgatcacct gtgctgcccc ggttgaataa tcgccgctat gcccggaata accgcccgcc
    10081 aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga agagcgccag
    10141 ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt tacgaatacg ctcatgttgc
    10201 tcatgaatca gttttaatgc ctgttcccag ctcacctgta cataggtatc ttcgccacga
    10261 cctttcgccg gttgcagtgg attgtcgaga tagctttttc tcaccatcgg atgctgaata
    10321 cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg tatggttttc
    10381 gccagcgcgc ctgtcgaaga aacaatcttg ccgtcattca cttctacgtt catcgctacc
    10441 caacgtcccg cggtgaggat tttaccgccc ttctcttctg cccatgccgg tagcggtgct
    10501 gccgatgtca ccaccagcgc tccagcggca ataccgctgt gtttaataaa ttcacgtctt
    10561 gttaatgtca taacttcctc cctgatcaac gaggatcact gtttctcggt aatatctttg
    10621 gcgttgtact ggaaataccg cgttaaaatg tccagttcgt tttcgctcat gctggttcgt
    10681 gcccccattc ctttggcaat ggacggccac gcattgacgg tgtaatggtc ggcggcaata
    10741 ggggcatgac aaccagcgca ataggtatcg tcaagttttt cagcgtattg ccatagcggt
    10801 ttacggtccg ctaatgcggg atcggtaagc gcaccctgta aagacgcctg acgccattga
    10861 ttgccgtatt cgtcagcctg ccattccccg tttaccgtga gcgccttgat accttcttca
    10921 cttaatgtgg ctagcgccag ccgttgacct gccgccaggt agagcgtgtt ttcactgccc
    10981 tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc cactggcatc aacgacggtg
    11041 agatcggtac caggattcac ggtagccagc tcgcctatgc gagaagtttt gaaaggataa
    11101 atatgtgcgc cattagtcac tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg
    11161 tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt
    11221 tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag attcactttg cgaggcaata
    11281 tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc ttttaattct
    11341 ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc actgtctatt
    11401 ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc aaataaataa
    11461 tccatccctg actttggaat atggcaatcg gcacattccg cacgtatccc tttctggttc
    11521 gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg gcaggaaagg
    11581 caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa cccgccgcca
    11641 ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc tcgcatagat
    11701 tcctcttatt aaattactgg ac
//
LOCUS       DAZUGK010000140        11720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZUGK010000140 DAZUGK010000000
VERSION     DAZUGK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.141"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..210
                     /locus_tag="ACHY5W_003340"
     CDS             <1..210
                     /locus_tag="ACHY5W_003340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448189.1"
                     /translation="KIAHCGYNDAAGEKMTSTPYTVHSCCAVCNYSTLSVLVYSLISR
                     LTSHKKPLRKLLILHGIIILPILVF"
     gene            141..434
                     /locus_tag="ACHY5W_003341"
                     /pseudo
     CDS             141..434
                     /locus_tag="ACHY5W_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(492..919)
                     /gene="tnpA"
                     /locus_tag="ACHY5W_003342"
                     /pseudo
     CDS             complement(492..919)
                     /gene="tnpA"
                     /locus_tag="ACHY5W_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            927..2135
                     /locus_tag="ACHY5W_003343"
     CDS             927..2135
                     /locus_tag="ACHY5W_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS4448190.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2179..2286
                     /locus_tag="ACHY5W_003344"
                     /pseudo
     CDS             2179..2286
                     /locus_tag="ACHY5W_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2270..2908)
                     /gene="ytfB"
                     /locus_tag="ACHY5W_003345"
     CDS             complement(2270..2908)
                     /gene="ytfB"
                     /locus_tag="ACHY5W_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFS4448191.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3127..3747
                     /gene="fklB"
                     /locus_tag="ACHY5W_003346"
     CDS             3127..3747
                     /gene="fklB"
                     /locus_tag="ACHY5W_003346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4448192.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4056..5468
                     /gene="cycA"
                     /locus_tag="ACHY5W_003347"
                     /gene_synonym="dagA"
     CDS             4056..5468
                     /gene="cycA"
                     /locus_tag="ACHY5W_003347"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFS4448193.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5513..6175)
                     /gene="ytfE"
                     /locus_tag="ACHY5W_003348"
     CDS             complement(5513..6175)
                     /gene="ytfE"
                     /locus_tag="ACHY5W_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFS4448194.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6283..7248)
                     /gene="ytfF"
                     /locus_tag="ACHY5W_003349"
     CDS             complement(6283..7248)
                     /gene="ytfF"
                     /locus_tag="ACHY5W_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS4448195.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7357..8217)
                     /gene="qorB"
                     /locus_tag="ACHY5W_003350"
     CDS             complement(7357..8217)
                     /gene="qorB"
                     /locus_tag="ACHY5W_003350"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS4448196.1"
                     /translation="MIAITGATGQLGHYVIESLMETVPASQIVAIVRNPAKALALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTLLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8306..8686
                     /gene="ytfH"
                     /locus_tag="ACHY5W_003351"
     CDS             8306..8686
                     /gene="ytfH"
                     /locus_tag="ACHY5W_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4448197.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8804..10747)
                     /gene="cpdB"
                     /locus_tag="ACHY5W_003352"
     CDS             complement(8804..10747)
                     /gene="cpdB"
                     /locus_tag="ACHY5W_003352"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFS4448198.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10937..11677
                     /gene="cysQ"
                     /locus_tag="ACHY5W_003353"
     CDS             10937..11677
                     /gene="cysQ"
                     /locus_tag="ACHY5W_003353"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFS4448199.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2777 a         2938 c         3053 g         2952 t
ORIGIN      
        1 aaaatcgctc attgtggata taacgatgca gcaggggaga aaatgacttc cacgccttac
       61 accgtacatt cttgttgtgc ggtatgtaat tattctacgc tgtcggtatt ggtgtattcg
      121 ttaatcagca ggttaaccag ccacaaaaag ccattgagaa aattattgat tttacatgga
      181 attattatat tgccaatcct tgttttttaa aaattgtgca tagtgaaaat caaagtaaag
      241 gtgtgcatta tgcaaaatca cagcgtctct tagaaattac ccatgcgcac ttgcagttaa
      301 tggaatcctt attggatgaa gggaagaaac acaatatttt taagccagat atcgacccat
      361 ttcaggtgaa tattaatatt gctgcgcttg ggggatatta tttgatcaac cagcatacgc
      421 ttggcctggt ttatccggtg cgccgtaaaa ccccgtcctt cagggcgggg atatacggcg
      481 cggttttcca cctaaccagg tgtttgctgt tgttcaatgt attgacggat ggcggatatt
      541 ggcgcacctc cgcagctact ggcaaaatag ccaggactcc acaaaacgcc tttgtagtaa
      601 tacctgactg caatatctgg tcgatctcgt cgcagtaacc tacccgatac gcctttgagg
      661 ctgtttacca gactggatat cgccagtttg ggaggatagt taattaacaa acgtgatctg
      721 gttcgccatc catttcaacc agttcagctt caaaatcagc acataccttt gaaaagtaag
      781 tgcgtagttt ttctgtcgcg tcatggtcaa aaatctggcg tcggtatctg gtgacaaaga
      841 ccaggtgaac atgcatcagg aaaacacaat gccttccacg ccggatatcg gtttcttttt
      901 tcacagacca aaatataatt tgacccgtga aacgactaca ggcatttaaa ttccagttaa
      961 gacccggtgg tcaacaggag cgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt
     1021 tcaatcgtgc tctggcgctt cagaatgaga atcatgaggc cggtaataaa tacatccctt
     1081 acgggaaaat ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg caatggctta
     1141 aagatgcccc gtcacagcca ttgcaacagt cactgaaaga ccttgagcgg gcttacaaaa
     1201 acttcttccg gaagcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgcat
     1261 tccgctaccc gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac
     1321 ttggctggat gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa aatgtcactg
     1381 tcagccagtc ctgcggtaag tggtacatca gtattcagac tgaaaatgaa gtatcaactc
     1441 cggttcaccc ttcagcatta atggtcggac tggatgctgg cgtggctaaa ctcgccacgc
     1501 tgtcagatgg cacagtcttt gggcctgtaa acagttttca gaaaaaccag aagacgctgg
     1561 cgagacttca gcgacagtta agccgcaagg tcaaattcag caacaactgg cagaagcaga
     1621 aacgcaaaat acagcgactg cattcccgta tcgcaaatat ccgcagggac taccttcata
     1681 aagtcacaac gaccgtcagc aaaaaccacg caatgatagt cattgaggat ttgaaggtca
     1741 gcaacatgtc aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa
     1801 aatcaggttt aaaccgttcg atactggatc agggctggta tgaaatgcgc cgccagcttg
     1861 agtacaagca gctctggcgt ggcggtcagg tgcttgctgt tccgccagcg tacacaagcc
     1921 agcgttgcgc gtgctgtggt catacagcga aagaaaatcg cctgtcacaa agtaaattca
     1981 gatgccaggc atgtggatat acagcgaacg ctgatgtaaa tggcgctcgt aacattttag
     2041 cggcggggca cgccgttctt gcctgtggag agatggtgca gtcaggccgc ccgttgaagc
     2101 aggaacccac cgaaatgatt caggcgacag cctgaacgta gcagggatcc acgtccttca
     2161 gggcgtggag gatgtcaaca catcagtatg gtttcacccc aggcactgga agccagacgt
     2221 aaggtcatca aagagacaat ccttagctgg cttttggttg acccgtcatc taccgcgcac
     2281 gaataaaact gccatccggc tggcgggtga acaggacctg ttgattattc cccgtatcaa
     2341 tggttaagcc cgtcaccacg ccgctggcgt tctggcggat tttcaccatc tgcccgtttt
     2401 gcaaattact cagcggctta cccgcgcctt ctacctgcgc cattgcatag acatcggtag
     2461 cgggcagtcc gtggtcgcgg aacagctgcg ccatcgtttt accgggttcg acacggtaag
     2521 agcgccattg attatcaatt ccgctatctg gctggaatgg ttgtgtttgg gtagtttgcg
     2581 gctgctcttc tggctggcct tcctgtatag gttctggagc gaccggagcc acctgatcag
     2641 gatcattttg tggtgtgact aattgcgccc gcagctgttc ttctgtcggc ggttgtgatt
     2701 gtgactgaat atctaactgc gcttcacgcg tcaccaccgg cgcgttgggc gtatcatcag
     2761 atggaagcag aaatccgacc accaatacaa tggcggcaat aatgatcccc cgacgatgca
     2821 tcggcggcag cgggtccatg aagcgaaaat tgtccggcgc gtgccagact ttctccaggg
     2881 ttggttttag ttcaaagcgc ccgggcatgg tttcctcctg ctccgcgtct tgttcctcaa
     2941 tcatagccta tgaataagct aacgctatga tgtccgtggt aaacccgcct ttattatatt
     3001 catacgggat attgctattg tttctttttc cccgggattt gtcatcattc ctgcgacaaa
     3061 gttttaccca aagaagtgtg gctgatatgc tgcccgctac ttttataccc taagaaagga
     3121 aatacgatga ccaccccaac ttttgacacc atcgaagcgc aagcaagcta cggcattggt
     3181 ttgcaggtag ggcaacaact gagtgaatct ggcctggaag ggctgctgcc agaagcactg
     3241 gttgcaggta ttgccgatgc gctggaaggc aaacatccgg ctgttccggt tgatgtggtg
     3301 catcgcgcgc tgcgtgaaat ccacgaacgc gccgatgccg ttcgtcgtca gcgtttccag
     3361 gcgatggctg ctgaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga aggtgtgaat
     3421 agcaccgaat ctggcctgca attccgcgtg atcaaccagg gtgaaggcgc aattccggca
     3481 cgtaccgacc gcgttcgtgt tcattacacc ggtaaactga tcgacggcac cgtgtttgac
     3541 agctccgttg ctcgtggtga acccgctgaa ttcccggtta atggcgtgat ccctggctgg
     3601 attgaagcac tgactctgat gccggtaggt tctaaatggg aactgactat cccgcaggaa
     3661 ctggcatatg gcgagcgcgg cgcaggcgcg tccatccctc cgttcagcac cctggtgttt
     3721 gaagtcgaac tgctggaaat cctctaagca gcgcattctg ttcccctcga accagagggg
     3781 agcaggcatt cagcaataaa cccttcagtt tgccaaacgg cgctattttg tgttgcaaag
     3841 accccgtaag cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata tgacactcac
     3901 tttaaacata aaattaacat tagatctaaa tcttagtatt catcccgcgt attgttacct
     3961 aatatcgatg agtcccgata cagattcgtc gtatcataga ctgactaaag gccgtagagc
     4021 ctgaacaaca cagacaggta caggaagaaa aaaacatggt agatcaggta aaagtcgttg
     4081 ccgatgatca ggctccggct gaacagtcgc tacggcgcaa tctcacaaac cgacatattc
     4141 agcttattgc cattggcggt gccattggta cggggttgtt tatggggtcc ggcaaaacga
     4201 ttagccttgc cgggccgtcg atcattttcg tttatatgat cattggtttt atgctctttt
     4261 tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa tcttttagtg
     4321 acttcgcttc cgatttactc gggccgtggg caggatattt caccggctgg acttactggt
     4381 tctgctgggt tgtaaccggt atggcagacg tggtggcgat cacggcttat gctcagttct
     4441 ggttccccga tctctccgac tgggtcgcct cgctggcggt gatagtgctg ctgctgacgc
     4501 ttaacctcgc caccgtgaaa atgttcggtg agatggagtt ctggtttgcg atgatcaaaa
     4561 tcgtcgccat tgtgtcgctg attgtcgtcg gcctggtcat ggtggcgatg cactttcagt
     4621 caccgactgg tgtggaagcg tcattcgcgc atttgtggaa cgatggcgga tggttcccga
     4681 aaggtttaag tggcttcttt gccggattcc agatagcggt tttcgctttc gtggggattg
     4741 agctggtagg tacaacagct gcggaaacca aagatccaga gaaatcactg ccacgcgcga
     4801 ttaactccat tccgatccgt atcattatgt tctacgtctt cgcgctgatt gtgattatgt
     4861 ccgtgacgcc gtggagttcg gtagtcccgg agaaaagccc gtttgttgaa ctgttcgtgt
     4921 tggtagggct gcctgctgcc gcaagcgtga tcaactttgt ggtgctgacc tctgcggcgt
     4981 cttccgctaa cagcggcgtc ttctctacca gccgtatgct gtttggtctg gcgcaggaag
     5041 gtgttgcgcc gaaagcgttc gctaaacttt ctaagcgcgc agtacccgcg aaaggattga
     5101 ccttctcgtg tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat cctagcgtga
     5161 ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct gtttatgttc gtctggacga
     5221 ttatcctttg ctcgtacctg gtgtatcgca aacagcgacc tcatctgcat gagaagtcga
     5281 tctacaagat gccgctcggc aagctgatgt gctgggtatg tatggcgttc tttgtgttcg
     5341 tggtcgtgtt gctgacactg gaagatgaca ctcgccaggc gctgttggtc accccgctgt
     5401 ggtttatcgc actggggctg ggctggttgt ttattggtaa gaagcgggct gctgaactgc
     5461 ggaaataacc gcattatcat gctggatggc gcaatgccat ccagctttta gatcactcac
     5521 ccgccagcgc gcgtgggaac agtacattgt tttccaggct gatgtgatcc atcaggtcat
     5581 caatcagttc attaatgccg ttatacatcg ctttccaggt ggtgcaggct tccggcggcg
     5641 gtgtgacgtt attggtagtg tgtttgatca cttccagcag ttcacccgct tcatcgtgct
     5701 cgctttccat tacgctgatt ggccccattg cctggctgcc catgccttgt ttgatcatcg
     5761 ggaagaggat ctgctcttct ttcatcatgt ggctggaaag ctcttcatgc agcatagtca
     5821 ggtattttgt cagccctttt ggtacgctcg gtttgtcggc gtgaacgcgc tcgactttag
     5881 tcgcttgcag gatcagctcc ggcagttgct cgcggtgacg atcgtggtag cgcacgatga
     5941 tatggtcaat gatttctgcc agcggggcgc tacgccagtc tttctcaatc ggttgttcag
     6001 caagctttgc cagttcagct tcaatgacct caacatccag ttctttacgt gccgccgcac
     6061 gcgccagcgt ttgcttaccg ccgcagcagt aatccatatc atatttacga aacagagccg
     6121 aagcgcgagg gatagagagc gccagttcac ctaaaggttg gtcgcgataa gccatagctg
     6181 atacctcatt tttaataaga taagatgtat tttaaatgca tctttaaggc aaaagctata
     6241 acccttacgt agcagaggct tatttaactc actgccagcg tgtcaacttt ctgagacaga
     6301 ttcagttaaa ggtttttctg gttttgctct gaccgcgatg accacgccaa tcactaacag
     6361 cgcgataccg ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa aggtgtacag
     6421 caaacctgcc agcgtttcga aaacaatcag cggcccgaga atcactgtcg gtaatcgctg
     6481 gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa agcacggcta tcgcaatcat
     6541 cagactaata aacaccagcg gacgggggcc aaagggtagg gagaagtccg gcgtttgcgt
     6601 attcagccag taacaagcga cgagatagcc gataagtgaa accggcagtg tgaccagcgc
     6661 ctgcgctgtt gcccacatca tcggatgttt gtcgggattt tcccgcaacc agcgggcgtt
     6721 gcgcagggca taccacgccc agcagaccac ggaaactaac gccagcacga tgccagaggt
     6781 ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac tcagcaatat tcacacacgc
     6841 caggccgatg ccaatacaaa tcagtgccgg ggcgagtttt ccccacgcga gtttgccgtc
     6901 gcgctggcta taaagcagat tggcaaagac aggaatcacc accggcaggg tgccgataat
     6961 catcgtggaa acaggcgcgc cagtacgttg aatggcactg gcaaggcaga agtaataaat
     7021 gaggttgccc atcatagtga gcatcaaggc ggtaagccag tcccgacgcg ccaactgacg
     7081 cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg ccagataacg
     7141 ccccatcgac tgcaacatcg ccgggtattc cggtacgatc aacggcccga caaaaataag
     7201 cccccacatc aaccctgcta acagggcgta cagcacgccg ctaatcatta ctggcatcca
     7261 ttggtctgtc agaagaaacg ttcaggaagc agacatgatt gctcccggca tcccgtcatt
     7321 atcagagagg atgcgggagg atgccacttt aattaactag ttattaacat taaaaagatg
     7381 gcttacgctt tcggctaacg ttgtcgttgg gtggccaatc agtttgctaa gcgttttgct
     7441 gtcatcaaac agaccgcctt tcgatgcgcc aacgtcagaa tccgccagca tatccgccag
     7501 tccgtcgggc agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg
     7561 ataggtaacc tgtttgccgc tctgtttggt cagctctgcc gctaacagtg tcaacgtcca
     7621 ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg
     7681 tgccgcagct gccgcataat ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc
     7741 accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta
     7801 ccagccgttg cgcagcaggg tgtaaacgat gccagaatca gccagcattt tctccgtctc
     7861 gatgtgctca tcggcgaggc cgagcgggga ggtatctgca tgtagcaggc tggtataagc
     7921 gataaatttc acgccagccg cctttgcggc attaataaca ttacgatgct gcggggcacg
     7981 ttgacccact tcgctggaag agatcagcag taatttttcc actccctgaa gtgcagatgt
     8041 cagtgcggct tcatcgccgt agtcagcctg acgcacggta atgccttgtg ctgccagggc
     8101 cagggctttt gccggattac gaacgatagc cactatttgg ctggcaggaa ccgtttccat
     8161 caaggattca ataacatagt gaccaagttg gccagtggca ccagtaatag cgatcatggg
     8221 aagtctccat cgttttgtct tgtgtttaaa tacgctaaca cctaaactta cttttagtaa
     8281 gtacgtacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga
     8341 gggtaatctc tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg
     8401 ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta gcgacctgcg
     8461 gcgcaaaatg ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca
     8521 ggatggtttt cttaaccgta tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct
     8581 cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact ggattgagtt
     8641 gaatttgccc gaggtgttag cggtgcggga tgaacgtgcg gcataactgc cggatacgcg
     8701 gcctacggtg tatgacttgt cgggcggata agatgcgcca gcatcgcatc cgccaataaa
     8761 tgccggatgc ggttgatgat ttataggccg aaaaagaagt gttttactta ctcaaatcca
     8821 cctgataaat cgcaaacccg atatcatcgg tcgcgacttt attcatcgga tattgccctt
     8881 tctctttaat aaacgctgcg gctttatctg acggggaagt ttcgaaacgg atatccagtt
     8941 tcttatcggc agctatcggt gctaaacgcc agttgttatc tgccgccggg tgaatttccc
     9001 ccgcacgttt cgactcatca gcaatccacg ctgccagcac cgagcggttc tcatccggtg
     9061 aagcaaaagc gatatggctg tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt
     9121 tattggtggc aacgaggaac atagcgttcg gatcaatcgg cttgccatta aaggtcaggt
     9181 tcttaatcct ttccgcattg gcattaatca tctggcactc gccgtcataa cgggcgggct
     9241 gggtaacatc aatctgataa ttcacaccat caataacatc aaagttatag gtgcggaaac
     9301 cgtcccagtt gatgagtgac tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc
     9361 cggcggagca ctccagccac tctttcacct ctttaccgct ggctttcacc acaatcagcg
     9421 tattgggata gagataaaga tcggcggcat tacggaaagt cagctgacct ttttccacct
     9481 ctacatagct tgccgggtca tttttgcgac caccaacttt aaacggtgcg gcagctgaaa
     9541 gcaccggcag ttttgccaga tccggatcgc cctgaatgta atgctcgaca tacgcttttt
     9601 gcgcgttgtt caccacctgc acggtcggat cgtcctgcac cagcgccaga tagctataca
     9661 tattgtcggc agatttaccg attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg
     9721 ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag ggatttttta ttagcgatgt
     9781 cgtaaatcgg tcgtgcttcc gctttcgctt gcgtcacctg ccatttaccg ctgtcattac
     9841 tgagttgtaa gtcgaccaca ccaagatgat cgccccacat gcctggcatt accgccggaa
     9901 caccattcag cgtgcctttg gcgatatcag ccccttcgat atcagcaaaa tctttacctg
     9961 ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac gcccggaatt tcactgaggt
    10021 aataaactga gttttccgcc atcactttat acggatcggc agacagcccg gaatgtgcca
    10081 gaacgacaac aacatcggca cctttctcgc gcatttcagg cacgtatttg cgcacggttt
    10141 cggtaatatc attcaccgtc acttttccgg ataaattagc tttatcccag cccatgattt
    10201 gtggtggcac gacgccaata tagccaatct tcagcgtctg tttttttccg tctttatcga
    10261 ccacttcggt gtctttaatt aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa
    10321 tgacgttggc atttacataa gggaatttcg ctcctgccag cgcatttttc aggtaatcca
    10381 gaccgtagtt aaactcgtgg ttgccaagcg ttccgacggt atagtccagc gtatttaatg
    10441 ccttatagac cgggtgaata tcgcctgctt ttaatccttt cgccgacatg taatctgcca
    10501 gcggactccc ctgaatcaag tcgccgttat caaccagtac gctgtttttc acttcattgc
    10561 gggcatcgtt aatcaggctt gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt
    10621 tgtaataatc gaaatccatc atgttgctat gcagatcagt ggtttccatg atacgcagat
    10681 cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact
    10741 taatcatcag ggacatcctt ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc
    10801 ttacttatag aacagtgaag aatgccacaa ttttacgctt tgaaaatgat gacactatca
    10861 cagttggcgc attcattaac gatagggtat aagtaaaaca ataagttaac accgctcaca
    10921 gagacgaggt ggagaaatgt tagatcaagt atgccagctt gcacggaatg caggcgatgc
    10981 cattatgcag gtctacgacg gggcgaaacc gatggacgtc gtcagcaaag cggacaattc
    11041 tccggtaacg gcagcggata ttgccgcaca caccgttatc atggacggtt tacgtacgct
    11101 gacaccggat attccggtcc tttctgaaga agatcctccc ggttgggaag tccgtcagca
    11161 ctggcagcgt tactggctgg tagacccact ggatggtacg aaagagttta ttaaacgtaa
    11221 tggcgaattc accgttaaca ttgcgctcat tgaccatggc aaaccgattt taggcgtggt
    11281 gtatgcgcca gtaatgaacg taatgtacag cgcggcagaa ggcaaagcgt ggaaagaaga
    11341 gtgcggtgtg cgcaagcaga ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag
    11401 ccgttcccat gcagatgcgg agctgaaaga gtatctgcaa cagcttggcg aacatcagac
    11461 cacgtccatc ggctcttcgc tgaaattctg cctggtggcg gaaggacagg cgcagctgta
    11521 cccgcgcttc ggaccaacga atatttggga caccgccgct ggacatgctg tggctgcagc
    11581 tgccggagcg catgttcacg actggcaggg taaaccgctg gattacactc cgcgcgagtc
    11641 gttcctgaat ccggggttca gagtgtctat ttactaaatt cagatggcag aaacagtgta
    11701 tttcctgatt ctgccatcct
//
LOCUS       DAZUGK010000141        11411 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZUGK010000141 DAZUGK010000000
VERSION     DAZUGK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.142"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..220
                     /locus_tag="ACHY5W_003354"
     CDS             <1..220
                     /locus_tag="ACHY5W_003354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448200.1"
                     /translation="KIRNHASEFTDLIILILIYFEKGIGIYAVFLKILIIGMDFRARK
                     GFYMDTLLIISSSHHQQFTSLFPFNNGL"
     gene            300..2723
                     /gene="pgaA"
                     /locus_tag="ACHY5W_003355"
     CDS             300..2723
                     /gene="pgaA"
                     /locus_tag="ACHY5W_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFS4448201.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2732..4749
                     /gene="pgaB"
                     /locus_tag="ACHY5W_003356"
                     /pseudo
     CDS             2732..4749
                     /gene="pgaB"
                     /locus_tag="ACHY5W_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            4742..6067
                     /gene="pgaC"
                     /locus_tag="ACHY5W_003357"
     CDS             4742..6067
                     /gene="pgaC"
                     /locus_tag="ACHY5W_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFS4448202.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLWVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYVVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6069..6482
                     /gene="pgaD"
                     /locus_tag="ACHY5W_003358"
     CDS             6069..6482
                     /gene="pgaD"
                     /locus_tag="ACHY5W_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFS4448203.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6532..7320)
                     /gene="phoH"
                     /locus_tag="ACHY5W_003359"
     CDS             complement(6532..7320)
                     /gene="phoH"
                     /locus_tag="ACHY5W_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFS4448204.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLNDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7841..9112)
                     /gene="efeB"
                     /locus_tag="ACHY5W_003360"
     CDS             complement(7841..9112)
                     /gene="efeB"
                     /locus_tag="ACHY5W_003360"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFS4448205.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPMGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRA"
     gene            complement(9118..10245)
                     /gene="efeO"
                     /locus_tag="ACHY5W_003361"
     CDS             complement(9118..10245)
                     /gene="efeO"
                     /locus_tag="ACHY5W_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFS4448206.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10303..11133)
                     /gene="efeU"
                     /locus_tag="ACHY5W_003362"
     CDS             complement(10303..11133)
                     /gene="efeU"
                     /locus_tag="ACHY5W_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFS4448207.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2994 a         2789 c         2674 g         2954 t
ORIGIN      
        1 taaaatccga aatcatgcat cggaatttac tgatttaatt attttaatcc taatttattt
       61 tgaaaaaggc attgggattt atgccgtatt cctgaagatc ctcatcattg gaatggattt
      121 tcgagcgaga aaaggatttt atatggacac tctactcatc atttcttctt ctcatcatca
      181 acaattcacg tctctcttcc cgtttaataa cggattatga ggtgcaaaaa tatctttctt
      241 ttcagttacc tgtaatttga tacagagaga gattttggca atacatggag taatacagga
      301 tgtattcaag tagcagaaaa aggtgcccga aaaccaaatg ggctttgaaa cttcttactg
      361 ccgcattttt agcagcgagt cccgcggcga agagtgctgt taataacgcc tatgatgcat
      421 tgattattga agctcgcaag ggtaatactc agccagcttt gtcatggttt gcactaaaat
      481 cagcactcag caataaccaa attgctgact ggttacagat tgccttatgg gccgggcaag
      541 ataaacaggt tattaccgtt tacaaccgct accgtcatca gcaattacca gcgcgtggtt
      601 atgcagctgt cgccgtcgct tatcgtaacc tgcaacaatg gcaaaactct cttacactgt
      661 ggcaaaaggc gctctctctg gagccgcaaa ataaggatta tcaacgggga caaattttaa
      721 ccctggcaga tgcgggtcac tatgatactg cgctggttaa acttaagcag cttaactctg
      781 gagcaccgga caaagccaat ttactcgcag aagcctatat ctataaactg gcagggcgtc
      841 atcaggatga attacgggcg atgacagagt cattacctga aaatgcatct acgcaacaat
      901 atcccacaga atacgtgcag gcattacgta ataatcaact tgctgccgcg attgacgatg
      961 ccaatttaac gccagatatt cgcgctgata ttcatgccga actggtcaga ctgtcgttta
     1021 tgcctacgcg cagtgaaagt gaacgttatg ccattgccga tcgcgccctc gcccaatacg
     1081 ctgcattaga aattctgtgg catgataacc ctgaccgcac tgcccagtac cagcgtattc
     1141 aggttgatca tcttggcgcg ttattaactc gcgatcgtta taaagacgtt atttctcact
     1201 atcagcgttt aaaaaagacg ggacaaatta ttccgccctg ggcgcaatat tgggttgcgt
     1261 cggcttatct caaagaacat cagccaaaaa aagcacagtc aataatgacc gagctctttt
     1321 atcacaagga gaccattgcc ccggatttat ccgatgaaga acttgcggat ctcttttaca
     1381 gccacctgga gagtgagaat tatccgggcg cgctaactgt cacccaacat accattaata
     1441 cttcgccgcc tttccttcgg ttaatgggca cgcctacgag catcccgaat gatacctggt
     1501 tacaggggca ttcgtttctc tcaactgtag caaaatatag taatgatctt cctcaggctg
     1561 aaatgacagc cagagagctt gcttataacg caccaggaaa tcagggactg cgcattgatt
     1621 acgcgagtgt gttacaagcc cgcggttggc ctcgtgcagc agaaaatgaa ttaaaaaaag
     1681 cagaagtgat cgagccacgt aatattaatc tggaggttga acaagcctgg acagcattaa
     1741 cgttacaaga atggcagcag gcagctgtct taacgcacga tgttgtcgaa cgtgaaccgc
     1801 aagatcccgg cgttgtacga ttaaaacgtg cggttgatgt acataatctt gcagagcttc
     1861 gtatcgctgg ctcaacagga attgatgccg aaggtccgga tagtggcaaa catgatgtcg
     1921 acttaaccac catcgtttat tcaccaccgc tgaaggataa ctggcgcggt tttgctggat
     1981 tcggttatgc cgatggacaa tttagcgaag gaaaagggat tgttcgcgac tggcttgcgg
     2041 gtgttgagtg gcggtcacgt aatatctggc tcgaggcgga gtacgctgaa cgctttttca
     2101 atcatgagca taaacccggc gcgcgccttt ctggctggta tgattttaat gataactggc
     2161 gtattggttc gcaactggaa cgcctctctc accgcgttcc attacgggca atgaaaaatg
     2221 gtgttacagg caacagtgca caggcttatg ttcgttggta tcagaatgag cggcgtaagt
     2281 acggtgtctc ctgggccttc actgattttt ccgacagtaa ccagcgtcat gaagtctcac
     2341 ttgagggtca ggaacgcatc tggtcttcac catatttgat tgtcgatttc ctacccagtc
     2401 tgtattacga acaaaataca gaacacgata ccccatacta caaccctata aaaacgttcg
     2461 atattgttcc ggcatttgag gcaagccatt tgttatggcg tagctatgaa aatagctggg
     2521 agcaaatatt cagcgcaggt gttggtgcct cctggcaaaa acattatggc acggatgtcg
     2581 tcacccaact cggctacggg caacgcatta gctggaatga cgtgattgat gctggcgcaa
     2641 cgctacgctg ggaaaaacga ccttatgacg gtgacagaga acacaactta tacgttgaat
     2701 tcgatatgac attcagattt taaggataaa tatgttacgt aatggaaata aatatctcct
     2761 gatgctggtg agtataatta tgctcaccgc gtgcattagc cagtcaagaa catcatttat
     2821 accgccacag gatcgcgaat ctttactcgc cgagcaaccg tggccgcata atggttttgt
     2881 agcgatttca tggcataacg ttgaagacga agctgccgac cagcgtttta tgtcagtgcg
     2941 gacatcagca ctgcgtgaac aatttgcctg gctgcgcgag aacggttatc aaccggtcag
     3001 tattgctcaa attcgtgaag cacatcgagg aggaaaaccg ctaccggaaa aagctgtagt
     3061 gctgactttt gatgacggct accagagttt ttatacccgc gtcttcccaa ttcttcaggc
     3121 cttccagtgg cctgctgtat gggcccccgt cggcagttgg gtcgatacgc cagcggataa
     3181 acaagtaaaa tttggcgatg agatggtcga tcgagaatat tttgccacgt ggcaacaagt
     3241 gcgagaagtt gcgcgttccc ggctcgttga gctcgcttct catacatgga attctcacta
     3301 cggtattcag gctaatgcca ccggcagctt attgcctgta tatgtaaatc gtgcatattt
     3361 tactgaccac gcacggtatg aaaccgcagc agaataccgg gaaagaattc gtctggatgc
     3421 tgtaaaaatg acggaatacc tgcgtacaaa ggttgaggta aatccacacg tttttgtttg
     3481 gccttatggc gaagcgaatg gcatagcgat agaggaatta aaaaaactcg gttatgacat
     3541 gttcttcacc cttgaatcag gtttggcaaa tgcgtcgcaa ttggattcca ttccgcgagt
     3601 attaatcgcc aataatccct cattaaaaga gtttgcccag caaattatta ccgtacagga
     3661 aaaatcacca caacggataa tgcatatcga tcttgattac gtttatgacg aaaaccgcca
     3721 gcaaatggat cgcaatattg atgtgctaat tcagcgggtg aaagatatgc aaatatcaac
     3781 cgtgtatttg caggcatttg ctgatcccga tggtgatggg ctggtcaaag aggtctggtt
     3841 tccaaatcgt ttgctaccaa tgaaagcaga tattttagtc gggttgcctg gcaattacgt
     3901 acccgctcag gtgtaaacat ctatgcgtgg atgccggtat taagctggga tttagatccc
     3961 acattaacgc gagtaaaata cttaccaaca ggggagaaaa aagcacaaat tcatcctgaa
     4021 caatatcgcc gtctctctcc tttcgatgac agggtcagag cacaagttgg catgttatat
     4081 gaagatcttg ccggacatgc tgcttttgat ggcctattgt tccacgatga tgctttgctt
     4141 tcagattatg aagatgccag tgcaccggct atcgcggctt atcagcaagc aggctttagc
     4201 gggagtctga gcgaaattcg acaaaacccg gagcaattta aacagtggac ccgctttaaa
     4261 agtcgtgcgt taactgactt cactttagaa cttagtgcgc gcgtaaaagc cattcgtggt
     4321 ccacatgtta aaactgcacg aaatattttt gcacttccgg taacacaacc tgaaagtgaa
     4381 gcctggtttg cacagaatta tgctgatttc ctaaaaagct atgactggac tgctattatg
     4441 gctatgcctt atatggaagg tgtcgcagaa aaatcggctg atcaatggtt aatacaactg
     4501 atcaatcaga ttaaaagcat ccctcaggct aaagacaaat ctattttaga attacaggca
     4561 caaaactggc agaaaaatgg tcagcatcag gctatttctt cgcaacaact cgctcactgg
     4621 atgagcctat tacaactgaa tggagtgaaa aactatggtt attatcccga caattttctg
     4681 cataaccaac ctgaaataga cgttattcgt cctgagtttt caacagcctg gtatccgaaa
     4741 aatgattaat cgcatcgtat cgttttttat attatgtctg gtgttatgca tccccctatg
     4801 cgtagcgtac tttcactctg gtgaactgat gatgaggttc gttttcttct ggccgttttt
     4861 tatgtccatc atgtggattg ttggcggcgt ctatttctgg gtctatcgtg aacgccactg
     4921 gccgtggggg gaaaacgcac cagctcccca gttgaaagat aatccgtcta tctccattat
     4981 cattccctgt tttaatgagg agaaaaacgt tgaggaaacc atacacgccg ctttagcaca
     5041 gcgttatgag aacattgaag ttattgccgt aaatgacggt tcaacagata aaacccgtgc
     5101 catcctggat cgcatggctg cacaaattcc ccatttgtgg gtcattcatc tggcgcaaaa
     5161 ccaggggaaa gccattgcgc ttaaaaccgg agctgccgcg gcgaaaagtg aatatctggt
     5221 gtgcattgat ggcgacgcgt tattagaccg cgatgcggcg gcatatattg tggaaccgat
     5281 gttgtacaac ccgcgtgtgg gtgccgtaac cggtaatcct cgtattcgca cacgttctac
     5341 cctcgtgggt aaaattcagg ttggcgagta ttcctcaatt attggtttga tcaagcgaac
     5401 ccagcgtatc tatggaaacg tatttaccgt ttccggtgtt attgccgcat ttcgtcgcag
     5461 cgccctggca gaagtgggtt actggagtga cgatatgatc accgaagata ttgatattag
     5521 ctggaagctg cagttaaatc agtggacgat tttttacgag cctcgggcgc tgtgctggat
     5581 attaatgcct gaaacgttaa aagggctgtg gaaacagcgc ctgcgctggg ctcagggcgg
     5641 tgcagaagta ttcctcaaaa atatgacaag gctatggcgc aaagaaaact ttcgaatgtg
     5701 gccactgttt tttgaatact gtctgacgac aatatgggcc tttacctgcc tggtcggttt
     5761 cattatttac gtagtccaac ttgcaggtgt accgttaaat attgaattga cacatatcgc
     5821 tgcgacacat actgccggaa tattattgtg tacgttatgt ttactgcaat ttattgtcag
     5881 cctggtgatt gagaatcgct atgagcataa tctgacttca tcgcttttct ggattatttg
     5941 gttcccggtt attttctgga tgctgagcct ggcaacgaca ttggtatcat ttacacgagt
     6001 catgttgatg cctaaaaagc aacgcgcccg ttgggtaagt cctgatcgcg ggattctgag
     6061 aggttaatat gaacaattta attattacga cccgacaatc gccagtacgt ttactggttg
     6121 attatgttgc cacaaccatc ttgtggacat tatttgcttt gttcatattc ttattcgcca
     6181 tggatctgct gacgggttat tactggcaaa gcgaggcccg aagccgactt cagttctatt
     6241 ttttgctggc agtggcgaat gccgtcgtgt taattgtctg ggcgctgtac aataagctgc
     6301 gttttcaaaa acagcagcat catgcagcct accaatatac gccgcaagaa tatgcagaga
     6361 gcttagcaat acctgatgag ctctatcagc aactacaaaa aagccacagg atgagcgtac
     6421 acttcaccag ccaggggcaa ataaaaatgg tggtttcaga aaaagcgcta gtccgggcat
     6481 aaacacccaa aacaaagccc ggttcgcccg ggctctgcac cgataataca cttaactgta
     6541 tgcatgtagc gtacgttggc aaagtgccga acgtacgcaa tcctctttac cgaatcggac
     6601 gatcccgacc atttcatctt cttcgaaacg ttccagtgcg tcatttaatc cggagcgcac
     6661 gccgcgaggt aaatcgcatt gcgtgatatc accgttaacg ataaccgtca cgttctcccc
     6721 gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat
     6781 gacaactgca ttttcaaagg tacgtccacg catataggcg aacggcgcaa tttccacctt
     6841 ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc cctaagcgcc ggaccagcac
     6901 gtcgtatacc gggcgaaaat agggagcaaa cttttctgcg atatctccag gtaagaagcc
     6961 aagatcttca tcggcttgca gaactggacg ggtgacgata atcctgtcga catccttatg
     7021 tatcagggcc tctgccgctt ttgctgcgct gatccaggtt tttccgcacc cggcttcgcc
     7081 cgtagcgaat atcagctgct tactctcaat agcctttaga tagtgcaatt gcgcttcatt
     7141 tcgcgcgagg atgggcgaag tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc
     7201 gctcatctgc acaagcgagg tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc
     7261 ccgtctcagc acacgttttg cctcgcgacg agctttgatc actgcttttt gtcttcccat
     7321 ggagagcacc ttgagttgtt tgtattcatc acacgcgccg ttggcagcgc gattatgcgc
     7381 acgaacatca gagggttggc ttccttgtaa gccatagttt gcttttggat aaaatgccga
     7441 aaaacggcta cgcgcaccgt ttacggcgtc ggtaacacat gaaaagaaag gatgaggttg
     7501 aaaatgcaaa gtgacgagat gactaccgga ggagaaaact ccgcgagtgg tggcgcgttg
     7561 attatctaaa acatgtccag tacaggacgt taccatccgc gatctccata gtgactgact
     7621 atcactgccg ggaacttccg ctgctactta ataagtacaa cagatctcgc attcattgca
     7681 acaatatatt cacttatatt taactataaa acaccatttt agtgacatta gtttctactg
     7741 gaaagatgac agagtgatga cagagatgaa aaaagctgtg tgctttcagc aggattcaca
     7801 tcaggcatct gcgcacaaat gccttatccg cctaaaaaca ttaggcccgc aataatgcgc
     7861 ttccgagata atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa ccgccgccga
     7921 taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc
     7981 ctttttccag atcgtgttgg tagcagacaa acagcaaccc catatccagt tgtccggagt
     8041 tggtgacccc caacgaataa ctgtagccgc gacgcagcat caggctggac tcactctccg
     8101 ccgtgcgggg attcgccagc cggatatggc tgtccagcgc gatcaccttc ccttccgggt
     8161 cgctggcgta atcaggcaca tcatgctcat gctgcattcc catcggcgca ccggtttgct
     8221 tatcacggcc aaaaattgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc
     8281 gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct
     8341 gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg
     8401 cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgccg
     8461 cgtgatcgga aataaaccct tcccgcttcc agcgcacact gagcaaatcc ggcgtgtgtt
     8521 tgatgatatc gcgcagtgca tggatcaccg tgtcctgggt gttggcgcaa atttgtagca
     8581 acacatcacc atggcataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct
     8641 gcagcttttt tggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca
     8701 ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga
     8761 gtggtggcag gcgcggattt ggcgtttctg gtgctgctcc gccctgagtc agaaaagcaa
     8821 aacgctgagt caacaagcga aacaaccgct caagatcggc tttatcgctg gcaagcacat
     8881 caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac
     8941 cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtacccggt gcactttgcg
     9001 ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagtgcacca atccctttca
     9061 gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc ttcatactgc ataacgctta
     9121 atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggccc
     9181 tttcagcgcg ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt
     9241 acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccagcag
     9301 ttccgggttg gctttttgca gttgtggacg cagcagatcg acaattttct gcgagccttc
     9361 aacgttagcc tggaaatccc acagatcagt gtggctgtat cgatcttctt caccgctgat
     9421 tttgctggcc gccacttcct caatcagtcc ggctgcaccg ccaaccactt ttgaaggtgg
     9481 gaaagccagt tcactgatgc gtttttgtaa atcgaccaca tcggtataaa gctgctcagc
     9541 gtactgatcc attcctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa
     9601 accggtgaat tttggatcag cggctttttg ctcgtaatca tcttcacggg cgtcaatgct
     9661 gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt
     9721 cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc
     9781 tttggtgtcg gtcaccagct gtgtggtttc cgccatgaca tacgctttat atgcagtaat
     9841 tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt
     9901 gacaatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg
     9961 ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac
    10021 caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt tctgaataat
    10081 gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgtt tatccgtcac
    10141 ggtcactttg acttgcggaa tatcagcggc gttagccata aaagcagaag agaacagcgc
    10201 agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct
    10261 ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgctacgcgg agcgagacgc
    10321 tgtcgcccct gcacgtggtg gcagagcaaa cgccaccagc gccgggatga gataaataaa
    10381 ccagacggcg acttcgctga cgctcggcgc ttcctgataa ccaaaaatac cttccattag
    10441 cgtgccgaac agcgagtgag ttgagagcac cgcgctcata tcaaaggcga tttcctgaaa
    10501 gtggttccac aatccggctt catgaaatgc gcgaatggca ccagctgcca gccctgcggc
    10561 gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc
    10621 gccccagtag agcaggaagc cgagcaccac ggcagtagca agaccgagca ttgcacccag
    10681 cggcggccaa atcccgacat cttgttgaaa tgccgccagc aggaaaaata ccgactccag
    10741 gccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc
    10801 acgctgcaat gcgctatcga ctgcctgttc cagttgcact ttgacgttgc gcgacacttt
    10861 gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc
    10921 aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga agatacccaa
    10981 gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacaccaa tccatcggcc
    11041 tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc
    11101 ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga
    11161 gtcaatctat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac gctaaagaaa
    11221 aatacaagcg gaatgtttat aaaatgatgt tggaatgtaa aaatgaggag atcaaatccg
    11281 gagaaaaaag cgattatttt aaggtattta gctgacgttt cgcccggcag atgaagcttg
    11341 tacgaatgtt agtgcccaca aactcgctgc agcgtgcccg aagggttgga ttttcagtta
    11401 gtcgtatcac a
//
LOCUS       DAZUGK010000142        11348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZUGK010000142 DAZUGK010000000
VERSION     DAZUGK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.143"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            326..907
                     /gene="mdaB"
                     /locus_tag="ACHY5W_003363"
     CDS             326..907
                     /gene="mdaB"
                     /locus_tag="ACHY5W_003363"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFS4448208.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            938..1252
                     /gene="ygiN"
                     /locus_tag="ACHY5W_003364"
     CDS             938..1252
                     /gene="ygiN"
                     /locus_tag="ACHY5W_003364"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFS4448209.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1300..3192)
                     /gene="parE"
                     /locus_tag="ACHY5W_003365"
     CDS             complement(1300..3192)
                     /gene="parE"
                     /locus_tag="ACHY5W_003365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4448210.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3221..3802)
                     /gene="yqiA"
                     /locus_tag="ACHY5W_003366"
     CDS             complement(3221..3802)
                     /gene="yqiA"
                     /locus_tag="ACHY5W_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFS4448211.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3802..4629)
                     /gene="cpdA"
                     /locus_tag="ACHY5W_003367"
     CDS             complement(3802..4629)
                     /gene="cpdA"
                     /locus_tag="ACHY5W_003367"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFS4448212.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4654..5076)
                     /gene="yqiB"
                     /locus_tag="ACHY5W_003368"
     CDS             complement(4654..5076)
                     /gene="yqiB"
                     /locus_tag="ACHY5W_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFS4448213.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVIYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5077..5706)
                     /gene="nudF"
                     /locus_tag="ACHY5W_003369"
     CDS             complement(5077..5706)
                     /gene="nudF"
                     /locus_tag="ACHY5W_003369"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFS4448214.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5911..7392
                     /gene="tolC"
                     /locus_tag="ACHY5W_003370"
     CDS             5911..7392
                     /gene="tolC"
                     /locus_tag="ACHY5W_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFS4448215.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTFARTTTSNGHNPFRN"
     gene            7540..8211
                     /gene="ygiB"
                     /locus_tag="ACHY5W_003371"
     CDS             7540..8211
                     /gene="ygiB"
                     /locus_tag="ACHY5W_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFS4448216.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8217..9377
                     /gene="ygiC"
                     /locus_tag="ACHY5W_003372"
     CDS             8217..9377
                     /gene="ygiC"
                     /locus_tag="ACHY5W_003372"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS4448217.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9415..10203)
                     /gene="ygiD"
                     /locus_tag="ACHY5W_003373"
     CDS             complement(9415..10203)
                     /gene="ygiD"
                     /locus_tag="ACHY5W_003373"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFS4448218.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10346..11119
                     /gene="zupT"
                     /locus_tag="ACHY5W_003374"
     CDS             10346..11119
                     /gene="zupT"
                     /locus_tag="ACHY5W_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFS4448219.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11177..11347)
                     /gene="yqiD"
                     /locus_tag="ACHY5W_003375"
     CDS             complement(11177..11347)
                     /gene="yqiD"
                     /locus_tag="ACHY5W_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFS4448220.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2804 a         3050 c         2818 g         2676 t
ORIGIN      
        1 gatctggttt tcgataccct ccagtaaatc tatttaaaat aatctgcgta acacgctgat
       61 attttcattt ttagttattc aacaccacaa ttatcaactc gcagaaagtc cacaccaacg
      121 ttgtaaatta accctgtgct agttggaatg atgagaaatt gctatcagta ttgtcatttt
      181 cagaaaatat ttatcctgca tcggtgagtc agagcaagat cagacttttg ctaaattcgc
      241 aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc
      301 aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa
      361 attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgcgg atggcacact
      421 gcgcgacctc gggcatgatg ttcgcatcgt tcgcgccgac agcgactacg atgtcaaagc
      481 ggaagtacaa aactttctct gggctgatgt ggtgatctgg cagatgccag gctggtggat
      541 gggcgcgccg tggacagtga aaaaatacat tgatgatgta ttcaccgaag gtcacgggac
      601 tctgtatgcc agcgatggtc gtacccgcaa agatccgtcg aaaaaatacg gttccggcgg
      661 cctggtacag ggcaaaaaat atatgctttc tctgacctgg aacgcaccaa tggaagcctt
      721 caccgaaaaa gatcagttct tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca
      781 taaagcaaac caattccttg gcatggaacc gctgccgaca tttatcgcta atgacgtgat
      841 aaaaatgccc gatgttcccc gctatactga agaatatcgc aagcatcttg tggaaatttt
      901 tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa tcgcagaaat
      961 ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt
     1021 tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg attgcgctgc
     1081 tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc agtgggaaag
     1141 catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca gcgaagccgt
     1201 aaaaggcgac gtgctggaga tgaatatccg tattctgcaa ccggggattt aaacctgcct
     1261 tgtttgcccg gccatcctga ccgggcaatg ttctttcctt taaacctcaa tctccgccat
     1321 gtcgcctttc tcttgcaacc agttgcggcg atcttccgag cgtttcttcg ccagcagcat
     1381 atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact gcaccagacg
     1441 gcgagtgttc ggatcaagcg tggtttcacg caattgcatc gggttcattt cccccagacc
     1501 tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt aattgctcaa gtacgccctc
     1561 tttctcttct tccgtcagcg cgtaataaac ctctttcccg agatcaatac ggtagagcgg
     1621 tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa
     1681 agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga ggatacagat
     1741 tttgccataa cgaagctggc tcagatcgtc gctgtcagga tcgataccga tcgctaccga
     1801 aatatcatgc acttcctgcg aggccagcac ttcgtcggaa gagacttccc aggtgttaag
     1861 gatcttacct ttcagcggca tgatcgcctg atattcgcga tcgcgcgcct gcttggcaga
     1921 tccgcctgcg gagtcacctt ccacaaggaa cagttcggta cggttaagat cctgcgcggt
     1981 acagtcagcc agtttgccag gcagcgccgg gccgctggtc agctttttac gcaccacttt
     2041 tttagccgca cgcatacgac gctgggcgct ggaaatcgcc atctctgcca gcagctccgc
     2101 cgcctgaacg ttttggttca gccacaggat gaaggcatct ttcaccacgc ctgaaacgaa
     2161 tgccgcgcac tgacgcgaag agagacgctc tttggtctgc ccggcaaact gcggatcctg
     2221 cattttcacc gacagaacat aggcgcagcg atcccagata tcttccgccg acagctttac
     2281 accgcgcggc agaatgttgc ggtattcaca gaactcacgc atcgcgtcca gcaggccctg
     2341 acgcagacca ttaacatggg taccgccctg catcgttggg atcaggttga cgtagctttc
     2401 ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc cagtccacag cttcagtatc
     2461 accagcgaaa ttaccgataa acggtttttc tggcagcgtt ggtaaaccgt ttaccgcttc
     2521 cgccaggtaa tcattcagac cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc
     2581 atctttaaag gtaatctcaa cgccagggca cagtactgct ttggccttca gcacatgcgt
     2641 caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac
     2701 gctggtacca gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct gcaccttttc
     2761 gccattttca aaggcgatgt tataaacctg accatcgcgg cgcacgttaa cttctacgcg
     2821 cttcgacagg gcgttaacca ccgaaatccc cacgccatgc aggccgccag agaactggta
     2881 atttttgtta gagaatttac cgcctgcatg cagacggcaa agaatcagtt caaccgccgg
     2941 tacaccctct tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa taacttctaa
     3001 cgactggtca gcatgtaaga ttacatccac gcgttttgcg tgacccgcca gtgcttcatc
     3061 gacactgtta tcaataactt cttgccccaa atggttaggg cgagtggtat cggtatacat
     3121 ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat cagcgttata
     3181 agtttgcgtc atggtttaag ttagcaattc gagttgatcg tcagagatgg tgcagaccaa
     3241 gaaaatcgac gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt
     3301 ctatgacagt ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa tccagcactt
     3361 catctcccgt ctgttgcagc agccagatca aatccggcgc ttccagcggg tcaatctgca
     3421 tgactttaag atcgtaaata tggcgtgact ctagcacata ttgctgcccg gtgtaggggt
     3481 tctcgttctg accgagatag tccgtcagca gttcaaacgg gcgcaccgcc gggtttacca
     3541 ccactgcggg cagcataaaa cattgtgaca accaggtggc gtaatatccc cccagtgacg
     3601 aaccgacaat acccagcgaa tcgccgccat gttccaggac aatggattcc agcagctctg
     3661 ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt
     3721 ccgccagcca gttttttaac aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt
     3781 gtaaataaag aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt
     3841 gtccgccagg cgatgcacct cggtggtcag cgtgccatca gcatgtaact cgagagtacg
     3901 ccagccgggc gcgatagtat ccagcgtaaa gttggaacag tgcggcttaa actgcacaca
     3961 ggtcgacggc gttgccagca ggcggcgacc gttccagtcg agatccagct cctgatgaat
     4021 atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc
     4081 cgcgttacgc aggctgtgtt gatcaagcca actacaaccc gcaggtagcg gatgatgatg
     4141 cagcagcagc aacgtatggc gctctggcgc atcagccagt ttacgttcca gccactcaag
     4201 ctgaaactcg cttagctcac cgtgcggcac gccaaacacc tggctatcca gcaacaggat
     4261 ttgccattgc tcaccaataa atacccgctt cgccggggag atacccgcat cctgtaacgc
     4321 gctgtacatc gcgggctgga aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa
     4381 acttgcgatg ccttcagcga aatgctgata ggccgcagag gattgatcct gcgctaaatc
     4441 acctgtcgcg acaatcaggt cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc
     4501 ctggtagctt tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg caaacaggtg
     4561 agtatcggta atttgtaaaa tcctgactct ggcctcacca gccagaggaa gggttaacag
     4621 gctttccaaa tggtgtcctt aggtttcacg acgctagtaa accggaatcg ccatcgctcc
     4681 atgtgctaaa cagtatcgca accagtccgc taaaaactgg ttaatttgat gcttttcgtc
     4741 gcgttgatgc aactttttat taggataatc ataccgcgct ttgaagcgaa aaatctgctg
     4801 gcttgaacac acttcagcca ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag
     4861 gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc accagggtag tgtatcgggt
     4921 cgattccaca atcgtcagcc gatattgtgc gtttgccacc tgatagatta cagtttcgcc
     4981 gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa aagttcatct cgcacaggcg
     5041 catcatttca ggaaagtcag gtgtgtaacg cttcatttat gcccactcat tttttaacgc
     5101 ttgatgatgc agctgcagcc attgcaaagc gatgaccgac gctgcgttgt cgattttccc
     5161 ctcttctacc cactggtatg cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt
     5221 ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc ccaccataat
     5281 tgacgaacgc tcactggtgc cccccgggct tgccaggaaa cttaacaccg gtttggtccg
     5341 tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc
     5401 accctcttca atcatcccgg caaccatctc cagtagccag ggggtttcac tggtgtcgta
     5461 tgcggcaatt cgaatctgct caatcagcac aacttcatca cgcactgggt caaagggtag
     5521 caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt acctcatgac tcatttgccc
     5581 gttgaataga cgatgacgaa atctataaag atctaatgaa aaaaagccgc gataaagtgt
     5641 ttctcgtgca ataatttcta catcgttttt gccaaatgta acgggcaggt tgtctggctt
     5701 aagcattgtt aatgtcctgg cactaatagt gaattaaatg tgaatttcag cgacgtttga
     5761 ctgccgtttg agcagtcatg tgttaaattg aggcacatta acgccctatg gcacgtaacg
     5821 ccaacctttt gcggtagcgg cttctgctag aatccgcaat aattttacag tttgatcgcg
     5881 ctaaatactg cttcaccaca aggaatgcaa atgaagaaat tgctccccat tcttatcggc
     5941 ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca agtttatcag
     6001 caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt
     6061 gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg tgcagattac
     6121 acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac cagtgcgtcc
     6181 ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa
     6241 aaagcagcag ggattcagga cgtcacatat cagaccgatc agcaaacctt gatcctcaac
     6301 accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta tacacaggcg
     6361 caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa cgtgggcctg
     6421 gtagcgatca ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa
     6481 gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat caccggtaac
     6541 tactatccgg aactggcggc gctgaatgtc gaaaacttta aaaccgacaa accacagccg
     6601 gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg
     6661 agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt accgactctg
     6721 gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa aacccgtggt
     6781 gccgctggta cccagtatga cgatagcaat atgggccaga acaaagttgg cctgagcttc
     6841 tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc ccagtacaac
     6901 tttgttggtg ccagcgagca actggaaagc gcgcatcgta gcgtcgtgca aaccgtacgt
     6961 tcctccttca acaacattaa tgcttctatc agtagcatta acgcctacaa acaagccgta
     7021 gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg tacgcgtacc
     7081 attgttgatg tgttggatgc gaccaccacg ctgtacaacg ccaagcaaga gttggcgaat
     7141 gcgcgttata actatctgat taatcagctg aatattaagt cagccctggg tacgttgaac
     7201 gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac taatccggaa
     7261 aacgttgccc cgcaaacgcc ggaacagaat gctattgctg atggttatgc gcctgatagc
     7321 ccggcacccg tcgttcagca aacattcgca cgcactacca ccagtaacgg tcataaccct
     7381 ttccgtaact gatgacgacg acggggcttc ggccccgtct gaacgtaagg caacgtaaag
     7441 atacgggtta tctgccgcat tcttccccct tctcgcttca atttcgacca gccatcctct
     7501 attctgatgg gtatttacca ctggtcccgg aagacaaaaa tgaaacggac aaaatccata
     7561 cgccacgcat cgttccgcaa aaactggagc gcacgccatc tgacaccagt cgctctcgcg
     7621 gttgccactg tttttatgct ggctggctgt gaaaagagtg atgaaacagt gtctctctat
     7681 caaaatgctg acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg
     7741 tacaacaatg cgctgaaaga agccgaacgt actgcgccga aatacgccac ccgtgaagac
     7801 tgtgttgctg aatttggtga aggtcagtgc cagcaggcac cagcccaggc tggcatggca
     7861 ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc gctgatggcc
     7921 ggttacatga tgggtcgtct gatgggcggc ggcgcgggat ttgcacagca gccgctgttc
     7981 tcctcgaaaa acccagccag cccggcttac ggtaaatata ccgacgcgac gggtaaaaac
     8041 tatggcgcag cccagccagg ccgcaccatg accgtaccga agacagccat ggcaccaaaa
     8101 ccggcgacca ccactaccgt tacccgtggc ggttttggtg aatctgttgc caaacaaagc
     8161 actatgcagc gtagcgcaac cggtacctct tctcgttcaa tgggtggctg ataccgatgg
     8221 aaagagtcag tattaccgag cgcccggact ggcgtgagaa agcccacgaa tacggtttca
     8281 attttcacac catgtacggc gagccgtact ggtgtgaaga tgcttactac aagttgaccc
     8341 tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact gcaccagatg tgcctgaaag
     8401 tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa attccgcata ccaaaacaca
     8461 cctggagttt tgtgcgccag tcatggctga cacaccagcc atcgctttat tcgcgtcttg
     8521 atctggcgtg ggatggcact ggcgaaccta aacttctgga aaataacgcc gatacgccaa
     8581 cgtcactata cgaggcggcg ttctttcagt ggatctggct ggaagatcag cttaacgccg
     8641 gtaacttgcc ggagggcagc gaccagttta acagtctgca agaaaaactg atcgatcgct
     8701 tcgttgagct gcgtgaacag tatggcttcc agttgctgca tctcacctgc tgtcgcgaca
     8761 cggtggaaga tcgcggaacc attcagtatt tgcaggactg cgcgacggaa gctgaaatcg
     8821 ctaccgagtt cctctacatt gacgatatcg ggttaggtga aaaaggtcag ttcacggatt
     8881 tacaggatca ggtaatttcc aacctgttca agctgtatcc gtgggaattt atgttgcgtg
     8941 agatgttttc caccaagctg gaggatgcag gcgtacgctg gctggaaccg gcgtggaaga
     9001 gcattatctc caacaaggca cttctaccgc tactgtggga gatgttcccg aatcacccga
     9061 acctgctgcc cgcttatttt gcggaagatg atcatccgca aatggaaaaa tatgtggtta
     9121 aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg
     9181 aagcagcgga aggtccgtat ggcgaagaag ggatgattgt tcagcaattc cacccgttac
     9241 cgaaattcgg cgacagctat atgctgattg gtagctggct agtgaacgat caacccgccg
     9301 gaattggtat tcgtgaagac cgtgcattga tcacccagga tatgtctcgg ttttatccac
     9361 atatttttgt tgagtaatca atgatgccgg atggcattcg ccatccggta attgttaacc
     9421 tatctgcacc gacagcatac ttaaactgcc catttctata ccatcgaccg gaatggtaat
     9481 tggctcctgc ccatcccacg cacctaagac atacagcaac ggcagatagt gctctggcgt
     9541 tgggttcgat aacgcgccac cttcatggtc gaggtaattt accagaggat gttgttccac
     9601 tggcccttgc cacgtcagat tcgctttcac atactcatta aacgacgtcg cccacggata
     9661 cggtgaacta tcaccgtgcc acttcactgt acgcaggtta tgcaccacgt tgccgctggc
     9721 gaccaacatt attccttcat cacgcagcgc cgcgagtttt cgccccattt cgaaatgcca
     9781 ggcggcaggt ttgctactat cgatactcaa ctgcaccatc gggatatcgg cgtcaggata
     9841 catcttaatc agcacgcccc aggaaccgtg gtcaaagccc caggcttctt tatccagcgt
     9901 caccgggacc ggcgctaaca gctcaaccag atgctgtgcc agcgcaggcg aacccggagc
     9961 aggataatgc gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg
    10021 cgtctccatc gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa ctaccacaat
    10081 cgcttgcggg cgtggcaatg tcatccccaa cgtctgccag ctgcgggtat acaaattatc
    10141 ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac gtgttgaaga
    10201 catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg cttgttcggc
    10261 ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga tctgcctgaa
    10321 ggtgaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca tactggcggg
    10381 ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct cgaaccgctt
    10441 actggcgttt tcgctaggat ttgcggcggg gatcatgttg ctcatctcat taatggaaat
    10501 gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg gaatgtttat
    10561 cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgcgc atccgcagga
    10621 tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag ccattctgct
    10681 cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg tcacggcaag
    10741 cagcaacctg gagctgggat ttggcatcgc actggccgtc gcgttgcaca atattcctga
    10801 aggtctggcg gttgcaggcc cggtttatgc agcaacgggt tctaaacgca cggcgattct
    10861 gtgggcgggg atttctggac tagcagaaat tcttggtggc gtgctggcgt ggttaatcct
    10921 cggtagcatg atttccccgg tggtcatggc ggcaatcatg gcggcggttg caggaattat
    10981 ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc ctaataataa
    11041 ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag tgctgctaca
    11101 aacggcggga attggttaaa agcaacatca gaagttgtcc agcgcaggga caacttctgc
    11161 tagcttcact aaagccttat tctacctttt cggctgtctc ttctctcgta ctgtttaagt
    11221 atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga caataacgtt
    11281 tcaaatgcag gaaataaccc accacaacaa gagcaatcaa tacaatccag taccaggcga
    11341 taaacatc
//
LOCUS       DAZUGK010000143        11340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZUGK010000143 DAZUGK010000000
VERSION     DAZUGK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.144"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(247..495)
                     /locus_tag="ACHY5W_003376"
     CDS             complement(247..495)
                     /locus_tag="ACHY5W_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4448221.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNDGLTILRATDKESAHKFVQETLQEARETADDWFIH"
     gene            complement(723..788)
                     /locus_tag="ACHY5W_003377"
                     /pseudo
     CDS             complement(723..788)
                     /locus_tag="ACHY5W_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1010..2695
                     /gene="btsS"
                     /locus_tag="ACHY5W_003378"
     CDS             1010..2695
                     /gene="btsS"
                     /locus_tag="ACHY5W_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFS4448222.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            2692..3411
                     /gene="btsR"
                     /locus_tag="ACHY5W_003379"
                     /gene_synonym="yehT"
     CDS             2692..3411
                     /gene="btsR"
                     /locus_tag="ACHY5W_003379"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFS4448223.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3458..3928
                     /gene="yehS"
                     /locus_tag="ACHY5W_003380"
     CDS             3458..3928
                     /gene="yehS"
                     /locus_tag="ACHY5W_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4448224.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(3968..4429)
                     /gene="yehR"
                     /locus_tag="ACHY5W_003381"
     CDS             complement(3968..4429)
                     /gene="yehR"
                     /locus_tag="ACHY5W_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFS4448225.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4554..6554)
                     /gene="yehQ"
                     /locus_tag="ACHY5W_003382"
     CDS             complement(4554..6554)
                     /gene="yehQ"
                     /locus_tag="ACHY5W_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4448226.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEKLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQTLWLGGISQPLIHYEAAFNRALQAAETCNWRWVSESLRQLRASVDA
                     FHARASHYNAGECLHQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     TPVAPLPSASVLPVPSTAHQLLIRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(6551..7687)
                     /gene="yehP"
                     /locus_tag="ACHY5W_003383"
     CDS             complement(6551..7687)
                     /gene="yehP"
                     /locus_tag="ACHY5W_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS4448227.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(7680..9959)
                     /gene="yehM"
                     /locus_tag="ACHY5W_003384"
     CDS             complement(7680..9959)
                     /gene="yehM"
                     /locus_tag="ACHY5W_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFS4448228.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(9970..11058)
                     /gene="yehL"
                     /locus_tag="ACHY5W_003385"
     CDS             complement(9970..11058)
                     /gene="yehL"
                     /locus_tag="ACHY5W_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS4448229.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2708 a         2889 c         2934 g         2809 t
ORIGIN      
        1 agaaatacat ctttgactta attcgggctt tgctgcaaac atatctgcta ttccttattt
       61 tataaatgca tttcccaatt ctggtttgca tacatttctt gtggataaaa taaacacttg
      121 tcatcaattt tgcaaccgta tgttaacaaa atgagttgct ataccgataa agggacactt
      181 ttgcttagtt aaccacacca ttttcttcgg acataggaaa ttcccggcat gtggacggga
      241 ttttagtcag tgaataaacc agtcgtccgc tgtttccctg gcttcctgaa gtgtttcctg
      301 tacaaatttg tgtgcggact ctttatctgt ggctcttaaa atagtgagtc catcattact
      361 ggcagatttg atgatgattt ctgcattgtc atatcgtttc ctgatacggc gcagcatttc
      421 ctgttgcagg gcaggaacag aaccttttgg cattttgctg attttttctt tagcgataca
      481 gctctcaaca cgcataacac cctcctgtaa ctgtggttat gtacaggtgt tatttttagt
      541 tgtatagata accagtgtca attgctgata ttgtctatgg ggcatcaatg gggcatgtat
      601 gggacacttt ttataggcga aatccgtcga agttcgtcga catcgaaaac gaattatctg
      661 tccaaccctt gaaaatcggc gctcctggac gatcttcgtt gatttttaaa aatgttgcgt
      721 cacgcgcgta acgtgacagg attaatatca caaagcaacg ccacttcacc aattgtgtaa
      781 agcgccatcg tctcaccctt gctcgcgagg tcccggttta actttagacg cagttttgcg
      841 aaccaggtag ttttgcccgt tttttgtgca tctatagggt gattttattt ttgccaggcg
      901 attttgagtg atcgtactca cgaattctca tttttctgca agagttcaaa gaaagttaaa
      961 cgcaggcaat gtatgttacg cgttttaaag ggaagtgtgg tttgcgggta tgtacgattt
     1021 taatctggtg ttgctgctgc ttcagcagat gtgcgttttt ttagtcattg catggttaat
     1081 gagtaaaacg ccattattca taccgttaat gcaggtcacg gttcgtctgc cgcataaatt
     1141 tctctgctac atcgtctttt ccatcttctg catcatgggc acctggtttg ggttgcacat
     1201 tgacgattct attgccaata cccgtgcgat aggcgcggta atgggcggct tactcggcgg
     1261 tccggtcgtc ggtgggctgg ttgggctgac cggcggctta catcgatatt cgatgggggg
     1321 catgaccgcg ttaagttgca tgatctcgac catcgttgaa ggattactcg gtggcctggt
     1381 acacagcatc ctgatccgcc gcgggcgcac tgataaagtc tttaacccca ttaccgccgg
     1441 tgccgtcacg ttcgtcgctg aaatggtgca aatgctgatc atccttgcga tcgcccgacc
     1501 ttatgaagat gcggtgcgtc tggtgagtaa tattgctgcg ccaatgatgg tcaccaatac
     1561 cgtcggcgcg gcgctgttta tgcgtatatt gctcgataaa cgcgcgatgt ttgaaaaata
     1621 cacttcggct ttttctgcca ctgcgctgaa agtggcagcc tcgacggaag gcattttgcg
     1681 ccaggggttt aacgaagtga acagcatgaa agtggctcag gtgctgtatc aggaactgga
     1741 tattggtgca gtcgcgatta ccgatcgaga gaaattgctg gcctttaccg gaattggtga
     1801 cgaccaccat ttacccggca aaccgatttc ttcaacttat actttaaaag cgattgaaac
     1861 cggtgaagtg gtctacgctg atggcaacga agtaccttac cgttgctctt tgcatccgca
     1921 atgcaaactg gggtcgacgc tggtaattcc gttgcgtggt gaaaatcagc gggtgatggg
     1981 caccatcaaa ttgtatgaag ccaaaaaccg tttattcagt tcaatcaacc gcacgttggg
     2041 tgaggggatt gcgcaattgc tttcggcgca gatccttgcc gggcaatatg agcggcaaaa
     2101 agcgatgctc acccagtcag agatcaaact gcttcacgcc caggtgaatc cccatttttt
     2161 gtttaatgcg cttaacacca ttaaagcggt gatccgccgc gacagcgaac aggccagcca
     2221 gctggtgcag tatctttcca cttttttccg caaaaactta aagcggcctt cggagtttgt
     2281 tactctcgcc gacgaaattg aacatgtgaa tgcttatctg caaattgaaa aggcgcgctt
     2341 ccagtcgcgg ttgcaggtca acattgctat tccgcaagaa ttatcccagc agcaattgcc
     2401 cgcgtttacc ctgcaaccga tagtggaaaa cgccattaaa catgggacat cacaactgct
     2461 ggatacaggg cgagtggcaa tcagcgcccg acgtgagggg caacatttga tgctggagat
     2521 cgaagacaat gccggtttgt atcaaccggt aaccaatgcc agtgggctgg ggatgaatct
     2581 ggtggataag cgtttacgtg aacggtttgg cgatgactat ggaataagcg tcgcctgtga
     2641 gcctgatagt tacacccgaa taacgttacg actaccatgg agggacgagg catgattaaa
     2701 gtcttaattg tcgatgatga accgttagca cgggagaacc tgcgtgtatt tttgcaggag
     2761 cagagcgata ttgaaatcgt tggagagtgt tcaaacgccg tggaagggat cggcgcggtg
     2821 cataaactgc gcccggatgt gctgtttctc gatatccaga tgccgcgcat cagtggtctg
     2881 gaaatggtgg ggatgcttga cccggaacat cgcccgtata ttgtttttct cactgcgttt
     2941 gacgaatacg caattaaagc ctttgaagaa catgcctttg attatctgct gaagccaatt
     3001 gatgaagcgc gactggagaa aacgctggcg cgattgcgtc aggagcgcag caagcaggat
     3061 gtttcgctgt taccggaaaa tcaacaggcg ctgaaattta tcccttgtac ggggcatagt
     3121 cggatttatt tgctgcaaat gaaagatgtg gcatttgtca gcagtcggat gagcggtgtc
     3181 tacgttacca gccacgaagg gaaagagggc tttaccgaat tgacattacg taccctggaa
     3241 agtcgtacac cactactgcg ctgccatcgt cagtatctgg ttaacctcgc gcatttacag
     3301 gagattcgtc tggaagataa cggccaggcc gagttgattt tgcgtaatgg cttaaccgtg
     3361 ccggtcagcc gccgttatct gaaaagctta aaagaggcga ttggcctgta aaagactgct
     3421 aaaatggctt tttgcctcat caacacctga aggcctcatg ctaagtaacg atattctgcg
     3481 cagcgtgcgc tacattttga aagccaataa taatgacctg gtgcgtattc tggcgctggg
     3541 taatgtcgaa gccaccgcgg aacagatcgc cgtctggcta cgtaaagaag acgaagaggg
     3601 ttttcagcgt tgtccggaca ttgttttgtc gtcattcctc aatggcctga tttatgaaaa
     3661 acgcggcaag gatgagtctg ctccggcact ggagccggaa cgtcgcatta ataacaacat
     3721 cgtgctgaaa aaattacgca tcgcgttttc gctgaaaacc gatgacattc tggctatcct
     3781 caccgaacag cagttccgcg tttcgatgcc ggaaattaca gcgatgatgc gcgcaccgga
     3841 tcataaaaac ttccgcgaat gcggcgatca atttttacgt tattttctgc gtggactggc
     3901 agcgcgccag catgtgaaaa aaggctaaaa aattggcggt catgtttaga gcatgaccgc
     3961 caaccgatta tttcacttct ttaaaaccag cggctttcat caccagttcc atttgcgcca
     4021 tagtgatacc ttttttggca tcttcagcag aaacgttgat tcctgaaata ccctgcaggg
     4081 ctttaaaatc cactttttcc atatcgatag tcacgttttc ctgcgcgtag gtatcggtat
     4141 aggttaattt ttcttcaaca cccgcgatgt ttttgtattt ggcgcttaac ggctcaagtg
     4201 tcttggcagc atcttctttg gtggttgcac caatggaggc aaattgaatt ttggtttcag
     4261 aagattgctt aagcaccttg tcacctttgt agacataggt aatggcaatt tcagtgccgt
     4321 tcagattggc gctgaatttc ttcgattctt ctttgtcacc gcagccagca agagagaaaa
     4381 ccagaacaga tgcaacaacg agggaaaaca gcttattgaa agccttcatg taaaactcca
     4441 ttttatttaa tcaaggaact ggtgactctc accaggggct atataggata tgcctaatac
     4501 cgtggcgtga gcagtccgga actggagtag aactcttagt aaaaagcact atttcatcct
     4561 tgttgctgaa gcatggggaa taattgttcg caaagcaaaa caccgttatt cattgcttct
     4621 acccgtgcct cgctttctgt attacgaaat tgtcccaaca catgtgccag ccgataaaaa
     4681 cccaccgcgg tgaggtcatt cgccagcaac tctgcctgac caatagcact ctgttcctga
     4741 tagcgccagc cgttatggag cagttgaata agtaacgcct ggcagcgtat cagcaactga
     4801 tgagcagtag acggcacagg caaaacgctg gcagaaggta gcggtgccac aggcgtagtt
     4861 tctgcgtcca gcgcccaggc acgggttttt gtcatcatca cccgtggttc cagtgtcaat
     4921 tgcccttcaa caaaactgac aaagccagaa accagacaca cggggtcgtc tgtttgttgc
     4981 aaaagcgccg ccatgcgttc aacggcataa ggtgcgctgg ctgaggctgg taatgataac
     5041 gtcagcacat tatcttcccc ttcgccgcta atgacctgcg catccagcgt ctggcggctg
     5101 ctgtcccaac cgagcgaaat acactcagcg accggcagaa taaataagtt atcgacctga
     5161 ttaagaggcc gtatgcaggc ggggggacgc tggcgtaaat attcccgcaa agccacaatg
     5221 cccggctggc gtaacggcgc gctcaacatt tgccaggcat caggcgacag cggcacaacg
     5281 ctgcttaagc ggttgcgggt agctaacagc agctcgccat cggcactgcg ttttgctgct
     5341 tgtgaaacaa tttgcccgcc cgccagtgcg ccagcctgaa aactaaacag ccgacgcgta
     5401 gctgccggtg agttttcctg ttcacttcgc ggccaactgc gcgaaaggtg caaaatactg
     5461 ccggtgtcgg gatcggtaaa ccagatgcgt aaaccataat gctcaatatc ctgccagcaa
     5521 cgcataccta aagacaccag ccgcagatga tcaagctttg cttctccggc aatgccagag
     5581 ccaacgaccg tgcgccacgg cacaggagga acttcaccaa tactgtcgcg ccgggccatc
     5641 tcttgtgcgc aatttaatcg actgtttaat gccgcaagct gatgtaagca ttctccggca
     5701 ttatagtggc tggcgcgggc gtggaaggca tcaacgctgg cgcgcagttg ccgtagcgat
     5761 tcactcaccc agcgccagtt gcaggtctct gccgcctgca atgcgcggtt gaatgctgcc
     5821 tcgtaatgga tgagcggctg gctgatgccg ccaagccata atgtctggct taattgctga
     5881 acatattgac gacacgcgtt gccttcttcg ctggcaaacg gatcgtcaga tgatgtgacg
     5941 tgttcgctgc gcatctgcca gattaaatgg ttaaattctg cttgctgcgc tttggcctcg
     6001 acgaaggcct gtaccgccag tacgacatgt tcgcaaagtg tgccttcaat acaatcacaa
     6061 cgggcgaaac gaatactgct gcgggaataa aaacgcacat cgctcatcgg taagcgggca
     6121 gagggaattt cacccggcgc acagaacaac tcaatggtga tgcctttagc gaccagcgcc
     6181 tgtgcgcgtt tgcgggtagc atcgggaagg gtagccagtt tttccagcca gattgccgga
     6241 tcccactctt cttctttttc cgtagactga gtggtggcac aaagtcgttg ataacttaac
     6301 accagcatca cgcgatgacg gcacataccg ttggccccgc aactgcactg agcctctttc
     6361 agtgcctggc cgttcgccag ctgggtacgg acaccgtcac tgaaggtggc gattaaagcg
     6421 tcgttctcat ggctgatctc cgggacgttg ccattttcca gttccttaag actgcgctta
     6481 acaaaaccgg cattgcttaa cgccgtcagg gcctgcggtg tcagttctaa taattccgga
     6541 cgtagtgaat tcatgactga agattctccg caagccatga tgccagctcg cccggcgtca
     6601 tggcggctat ttgtgcgccg acattaacca gcgcctgggc cgtatcgcgg tcatagcaag
     6661 gtgttgcggt gctatcgagc gctgccagtc ccagcacttt gatgccgctc tggacacact
     6721 ttttcacctg atgcgtcagt aatgatgatg aacccccttc gtaaaaatcg ctcacgagga
     6781 taatgacgct tttcgctggt tgttcaataa gttgccgacc atactccacg gcactggcga
     6841 tattggtccc gccgcccaac tgtactttca ttaataactc taccggatcg gcaacgtctg
     6901 ccgtgagatc aacgacgctt gtgtcaaacg ccaccagatg ggtacgaatg ccgggtaact
     6961 gccacaaaca ggccgccatc accgcagagt ggatcaccga atcgaccatc gatccgcttt
     7021 gatcaaccag taagaccagt tgccattgtt cgctttggcg tttaatgcgg ctgttaaagc
     7081 ggggggattc gatatacaac ttgccgtgtt gcgggtgcca gtgttgcagg ttggcgcgca
     7141 gagtactttt gaaatcaaag tttcgcgcca gtggaataaa tgaacggcga cggcgatcgc
     7201 ggacaccaga aaaagcctga cgaacttcct ttgccagtcg agccataatt tcttcaacaa
     7261 cctggcacac tatccggcgg gcggcagcca gtacttcggg gttcatcaga tgtttggtgt
     7321 gcaaaacagc gcgtagcagg ctttcagaag gctgcatacg ttccagcacg tcgagattcg
     7381 tcaccacatc ttcaatgccg tagcgcagca cggcatcgct ttccagccgc tcaatcacct
     7441 gctgcggaaa cagcgtgtga atactgttga tccactcagg ggtggtgaga tttgagccac
     7501 ctaatccacc ggaacgttca ccacgctgga gccgttcagg atcgcgccca tacagccact
     7561 ccagcgcgtg gtctatctgc cgggcgttgt catccagccc acaaagcgtc gtttctgccg
     7621 cttcgccaag aattaatcgc cagcgttgta gctcacgggt ggtcagaaga tcgttcagtt
     7681 cagacatgga aaactcccca gttttgcagc gatgccagtg cctgctgttc gagttgttga
     7741 tgatgtgcaa tggcctgtgg tggacaatgc aacggcattt gcagcgccga aacgggaagt
     7801 tgcgccagtt gataatgctc aagcacctga tgcgccagcg tcccgcgttc tcgtggtggt
     7861 agccaggcca tcgccgcgcg taaatcgggc agggcgttaa taaaatcggc ttcactcagt
     7921 tgatttaaat gactgctgaa accggcgata aatgccggct gacaggccag ttgatggcgg
     7981 gccagcgcca gcaaaccgtg cagcgcctca ccagattgtg ccggggataa ctgcgccagc
     8041 atcgtcagcg ccgcttcggc actggcgttg ggatgttcca gacgcattag tgcgccaaga
     8101 gccgcgccgc gatccagcgc cggggcatga attgcctgac tgcgtcgctc cagtaaggct
     8161 accgccgcag aaagagaaac gccgggtaaa ttaacgccgc tatgtagatc gcgcagaata
     8221 tggcaaagcg cttgccagct atgcaggtga gcgtgaaact ccttttcatc cggtctgccg
     8281 ttagattcac acagccacag cgtgcgatcg atagtcgcgc ataacgtcgt ttgtaatatc
     8341 tgcgcgcctt gcataccgct aatttcatcc agccgccata aggcataaag cacttccagc
     8401 gcctggccca tttcggcaaa ttgattttcc tgggcgatta atagtgtgag ttgctccagt
     8461 aattgttgac tgaaggacgc cagacccgct aacgccgcct ggctaagaca tgtggtgata
     8521 ctgccgattc cgcccgcgtc cagcatatcg gcttctaatt tattgcgtgc ggcttcctgg
     8581 agtgtggcac caaaacaggc ggcctcaatc aatgccgcat gctggctaag cgggcgggtt
     8641 aatttccagc gttcttcacc gttgcctgca agtgtcagtg tacttccctg ctggcgtaca
     8701 atcccaggga tctccagtat tgccagccga tgtaacacct gactttgcgc cagcccatcg
     8761 ggggtaaagc gatttaaggt aagctccgcc ggaagggaga ttgcggtacg ttccagttcg
     8821 caggtgacat cttttggcag aggcggttgt ggcgtagaag gggcaagttt accgaatccg
     8881 tcacccgcta atgtgtctat caacgttagc agaatcggat cggtatcggg atgaataacg
     8941 ccgcgatagc tccacggcaa cggcgcgttc agggcttctt taatcagcga gcctgctatg
     9001 gcatccagcc agtcagtgcg taacggtagt gtatgaccgc gcaactgtgc cagcgccatc
     9061 gcatgcagat gagcggcagc catatccgcg gtagaagcgg gcaatttgtg ctggcgcaaa
     9121 cgggtaagga tagtttttag cagttgttca ccggccttct gcaagcccca ctgccagcac
     9181 cagttttgcc ataccggggc aggcattcct gaaaggtatc ctgccagcac atcaaggcgc
     9241 ttttcactgt agggtgtgag ataacaacct gtaacggcat ctgccagcga gggcaattct
     9301 ggcttgttaa ttttctgagg gcattcgcgc cacatctttg ccagtgccgg agcgtgccag
     9361 ccaccgcaga caactaacac gtcgccatta ttttgctgca tcgcccatcc aatccagcgg
     9421 gccataaagg cttcgcgctg acgattgagc gcatcgccgg aggcgtcgcc ccgcagttgg
     9481 gcaaaatagt gcgccagcgc agagggtaat gcagtttgct ggctttcatc ttcgaacaag
     9541 tgatcccaca gggtatcgct gttatccatg cgggtggcac gcagcagtaa ggcctggctt
     9601 tcatcttgcg tatcaggcga atcgtcctct tcttcactct gcgcccagca aggcaaatcg
     9661 atgaagtaag tttgtgcctg aatgcgacgt gcggcttgta gcgcctgcca ctccggcgaa
     9721 aattcagcaa atggcgtcca ggcaccacgc ccgggggctg caccgtcctg atactggcaa
     9781 taactgtaaa tagctaccgg aagctggtgg gctaaaaaca gttcgtctac ccggtcatta
     9841 aaatcagccg ggccttcaat caacacgtat cgtggccgct ggctttcgat taacgatttc
     9901 accagccggg cgcaggccgg actatgatgc cggatgccga caattaacgg ctcgctcatg
     9961 catctttcct cacggcaggc ggtggcgggc ttgataataa gcctgccagt gagcttcttt
    10021 atgtgtggcg acacgctgtt cgaagtaacg gcgcagacgc gcgcgatctt cttcgttatc
    10081 tttgacgatg gttccggcaa tacattccac caggtcggca ggctcccccg cgcgattcgc
    10141 taaaaaccag gcgcggacgc ccacagcatg ggccacgttc acggcttcag cggtggacat
    10201 aatcgctgtc agtgtatcca ttgacgtttt cttttcgccg ttggcacgca gatcgcggaa
    10261 ggtgcggacc agtaattcca gtaccgcgtc aggcacttta tgcggaatac cgctatgggc
    10321 cagcaaacgc gccgaagcac tggcgaccag ttccaactcc tgggcgaaat ccataatcgg
    10381 aaaaaccgtt tcaaaatcga agcgacgctt tagcgcagca ctcatttcat tgacgccgcg
    10441 atcgcgggta ttggcagtgg caataatatt aaagccttct cgcgcataga gctgactggc
    10501 ttcaccagtg agttccggca ccgtcatcac cctgtcagaa agcataccta acagacagtc
    10561 ctgcacttcc agcggtgtac gcgttatctc ttcgaaacgg acgattttgc cgtcgcgcat
    10621 tccctgataa agcggggcag ggaccagcgc ttccgttgac ggaccatgat tgataagcag
    10681 ggcgtagttc cagccatact tgatttgatc ttcggtggta gatgccccgc cctggatggt
    10741 taacccggca tcgccgctaa tagcggttgc cagtaattca gaaagaagag attttgcggt
    10801 acccggttcc cccaccaaca tcaaaccgcg gccggtagca agagtgacta acatacgttc
    10861 gacagcggag gggctaatca ccactttacg gctgatattt tgcgcttcgt cgccaaggat
    10921 aaaagcacgg gccgcaggca aacttaactg ccaaccgggc gggcaaggtg cgttatcatt
    10981 ttgttttaat tttgccagtt catcggcgta taacacagca gcaggcggac gctgcagatg
    11041 attattctgc ggtgacatat attcatccct gaaatgtatt aatgaaataa acgatatttt
    11101 acgcgcaata aatgtttggc gcattagttg tttgagcgaa aaatatctgg aaagcagatc
    11161 acagtaaata aaaaaacctg ctggtgaggc aggttctttt atttactatt ttcaggccta
    11221 acaccacagc ctgtgcaaca tcgtgttcca acgaacgaat tataaccacg gctggacccc
    11281 gttgcaatta agataatgag atttataatc caggccattg ggacgttgcg ccggatcgga
//
LOCUS       DAZUGK010000144        11337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZUGK010000144 DAZUGK010000000
VERSION     DAZUGK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.145"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            214..1080
                     /gene="folD"
                     /locus_tag="ACHY5W_003386"
     CDS             214..1080
                     /gene="folD"
                     /locus_tag="ACHY5W_003386"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFS4448230.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1082..1294
                     /gene="ybcJ"
                     /locus_tag="ACHY5W_003387"
     CDS             1082..1294
                     /gene="ybcJ"
                     /locus_tag="ACHY5W_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFS4448231.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1402..1923
                     /locus_tag="ACHY5W_003388"
     CDS             1402..1923
                     /locus_tag="ACHY5W_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4448232.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLGWRRR"
     gene            complement(1959..3344)
                     /gene="cysS"
                     /locus_tag="ACHY5W_003389"
     CDS             complement(1959..3344)
                     /gene="cysS"
                     /locus_tag="ACHY5W_003389"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4448233.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3518..4012
                     /gene="ppiB"
                     /locus_tag="ACHY5W_003390"
     CDS             3518..4012
                     /gene="ppiB"
                     /locus_tag="ACHY5W_003390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFS4448234.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4015..4737
                     /gene="lpxH"
                     /locus_tag="ACHY5W_003391"
     CDS             4015..4737
                     /gene="lpxH"
                     /locus_tag="ACHY5W_003391"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFS4448235.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4855..5364
                     /gene="purE"
                     /locus_tag="ACHY5W_003392"
     CDS             4855..5364
                     /gene="purE"
                     /locus_tag="ACHY5W_003392"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4448236.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5361..6428
                     /gene="purK"
                     /locus_tag="ACHY5W_003393"
     CDS             5361..6428
                     /gene="purK"
                     /locus_tag="ACHY5W_003393"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4448237.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITALPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6565..7458)
                     /locus_tag="ACHY5W_003394"
     CDS             complement(6565..7458)
                     /locus_tag="ACHY5W_003394"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4448238.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQIPPVT
                     TVLTRIEVSPDEPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7455..8270)
                     /locus_tag="ACHY5W_003395"
     CDS             complement(7455..8270)
                     /locus_tag="ACHY5W_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS4448239.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8281..9540)
                     /locus_tag="ACHY5W_003396"
     CDS             complement(8281..9540)
                     /locus_tag="ACHY5W_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS4448240.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9550..11217)
                     /locus_tag="ACHY5W_003397"
     CDS             complement(9550..11217)
                     /locus_tag="ACHY5W_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS4448241.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPADAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIAKLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2529 a         3234 c         2998 g         2576 t
ORIGIN      
        1 tcttaatctt tagacaagga aataaatcag ttccagattt acaacgccat catggacgaa
       61 aaatgaagct ttcaatctca gcgacggtgc gcctaacctt cgcaagaggc gcttcacgcg
      121 ataaatctga aacgaaacct gacagcgcgc cccacttctg acaaaatagg cgcatcccct
      181 tcgacctacg taacagatgg aatcctctct ctgatggcag caaagattat tgacggtaaa
      241 acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag ttcaggcgcg tattgcagcc
      301 ggactgcggg caccaggact ggccgttgtg ctggtgggta gtaaccctgc atcgcaaatt
      361 tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt tcgtctcccg ctcttacgac
      421 ctcccggaaa ccaccagcga agcggagctg ctggagctta tcgatacgct gaatgccgat
      481 aacaccatcg atggcattct ggttcaactg ccgttaccgg cgggtattga taacgtcaaa
      541 gtgctggaac gtatacaccc ggacaaagac gtagacggtt tccatcctta taacgtcggt
      601 cgcctgtgcc agcgtgcgcc gcgtctgcgt ccctgcaccc cgcgcggtat tgtcacgctg
      661 cttgagcgtt acaacattga taccttcggc ctcaacgccg tggtgattgg cgcatcgaat
      721 atcgttggcc gcccgatgag catggaactg ctactggcag gttgcaccac caccgtgact
      781 caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg ccgatctact gatcgttgcc
      841 gtcggcaagc caggctttat tcctggtgac tggatcaaag aaggcgcaat tgtgattgat
      901 gtcggcatca accgtctgga aaatggcaaa gttgtgggcg acgtcgtgtt tgaagacgcg
      961 gctaaacgcg cctcatacat tacgcctgtt ccgggcggcg ttggcccgat gacggttgcc
     1021 acgctgattg aaaacacgct tcaggcgtgc gttgaatatc atgatccaca gggtgagtaa
     1081 catggcgaca ttttctttag gtaaacatcc gcacgttgag ctgtgcgact tgctgaaact
     1141 ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg attgccgaag gccaggtgaa
     1201 agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc gtcgccgggc agacagtgag
     1261 ttttgcaggt cacagcgtac aggttgttgc ctgatccctt tcccggcctg tatcaacagg
     1321 ccggttctct tttcagactt ttgccctcta cgccccgttc atccatgctg atagcatcga
     1381 cattttgccg aagcgaccat catgccaacc gttattacgc acgccgccgt tcccctatgc
     1441 attggtttag ggttgggttc gaaagtcatc cctccgcgtc tgttatttgc cggaatcatc
     1501 ctggcgatgc tgcccgacgc cgacgtattg tcgtttaaat ttggcgttgc ttacggcaat
     1561 gtttttggtc atcgcgggtt tacccattcg ctggtgtttg cgtttgttgt cccgctgtta
     1621 tgtgtgctga ttggacgccg atggttcagg gctgggctga ttcgctgctg gctgttttta
     1681 accgtctcgt tgctatcgca cagcttgctg gattcagtaa ccactggcgg taagggcgtt
     1741 ggctggctgt ggccgtggtc agatgaacgc tttttcgctc cctggcaggt gattaaggtc
     1801 gcgccgtttg cattgtctcg ttacaccacg ccgtacgggc atcaggtgat aatttctgaa
     1861 ttgatgtggg tgtggttgcc ggggatggtg ctgatgggaa tgttggggtg gcgcagacga
     1921 taaccggatg cgaaaactcg catccggcaa tagcgcaatt acttacgacg ccaggtggtc
     1981 ccttgcgggc catcttccag cacgatcccc atctcgttaa gacggtcacg cgccgcatct
     2041 gccgccgccc agtctttcgc tttacgggca tccagacgct gttgaattaa cgcttcaatc
     2101 tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca ggaacgcttc cggttcttgc
     2161 tccagcaggc ccaatacggc ggaaagttta cgcaggtgag atgccattgc attcgccgct
     2221 gccatatctt ctgctttcag acggttcact tcacgcgcca tatcaaacag cacggaatag
     2281 gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa agcgcgcttc aaacgcttcg
     2341 ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg cagtgtagag acgctccagc
     2401 gccgcacgcg cctgcttcag gttctcttcg ctgtagttca actggctgcg atagtggccc
     2461 gacatcagga agtaacgcac ggtttcggcg tcgtagtatt tcagcacatc gcgaacggta
     2521 aagaagttac ccagcgattt ggacattttc tcgcggtcaa ccatcaccat tccggagtgc
     2581 atccagtagt tcacatactg gccatcatgg gcacaggtgg actgcgcgat ttcgttctcg
     2641 tggtgcggga acatcaggtc tgaaccgccg ccgtggatat caaagtggtt acccagctgc
     2701 ttgcagttca ttgccgaaca ttcaatgtgc cagcctggac ggcccgcgcc ccacggagac
     2761 ggccagctcg gttcgccctc tttcgacatc ttccacagaa cgaagtccat cgggttgcgt
     2821 ttgtcgtcga ccacgtcaac gcgcgcgcct gcctgcagct ggtcgagatc ctgacgcgac
     2881 agcacgccat aagtcggatc ggtcgggacg tcgaacatca cgtcgccgtt gtccgccaca
     2941 taagcgtgac ctttggcgat cagttgttca gtgagttcaa taatttctgc gatatggtgc
     3001 gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag catcaaaatc tttgtgcatt
     3061 tcggcgatca tgcgatccac cagcgctaca aagctttcgc cattttcatt ggcgcgtttg
     3121 atgattttgt cgtcgatatc ggtaatgttg cgcacatact tcagcttata gccgaggaaa
     3181 cgcagatagc gtgcaaccac gtcaaaagca acaaaggtac gcccgtgacc gatatgacag
     3241 agatcgtaaa cggtgattcc acacacgtac atgccgactt ccccggcgtg aataggctta
     3301 aattcctctt tttggcgtgt cagagtattg aagattttta gcatcgaaga ttccgtttag
     3361 acatgtgtgg gtaattgagt tgcgtataat acccatattt cccgcgcgaa tcagcataca
     3421 ttgcgtgatg atccgatcgt gcggttatgc tataacacca ccctatatat gacccgaacc
     3481 gggttgaagc accaatcaaa cggaacagga tgcaaaaatg gttactttcc acaccaatca
     3541 cggcgatatt gtcatcaaaa cttttgacga taaagcacct gaaacagtta aaaacttcct
     3601 ggactactgc cgcgaaggtt tttacaacaa caccattttc caccgtgtta tcaacggctt
     3661 tatgattcag ggcggcggtt ttgaaccggg catgaaacaa aaagccacca aagaaccgat
     3721 caaaaacgaa gccaacaacg gtctgaaaaa tacccgtggt acgctggcaa tggcccgtac
     3781 tcaggctccg cactctgcaa ctgcacagtt cttcatcaac gtggttgata acgacttcct
     3841 gaacttctct ggcgaaagcc tgcaaggttg gggctattgc gtgtttgctg aagtggttga
     3901 aggcatggac gtggtagaca aaatcaaagg tgttgcaacc ggtcgtagcg gtatgcacca
     3961 ggacgtgcca aaagaagacg ttatcattga aagcgtgacc gttagcgagt aatcgtggcg
     4021 acactcttta ttgcagatct tcatctctgc gtggaagaac cggcgatcac cgccggtttt
     4081 ctgcgttttt tagcggggga agcccgcaat gccgacgcgc tgtatattct tggcgatctg
     4141 tttgaagcgt ggattggcga cgacgatccc aacccactcc atcgccagat ggcggcagcg
     4201 atcaaagcgg tgtccgattc cggcgttccc tgttatttca ttcatggcaa ccgtgatttt
     4261 ctgctcggca aacgctttgc ccgtgaaagc ggcatgacgt tattgccgga agaaaaagtg
     4321 ctcgaacttt atggtcgtcg ggtgctgatt atgcatggcg acacgctgtg caccgatgac
     4381 gcgggttatc aggcttttcg cgccaaagtc cacaaaccct ggctgcaaac gctattcctc
     4441 gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa tgcgcgcgaa cagtaaagaa
     4501 gccaacagca gtaaatcgct ggcgatcatg gacgttaacc aaaacgcggt ggtcagtgcg
     4561 atggaaaaac atcaggtgca atggctgatt cacgggcata cccatcgccc ggcggtacat
     4621 gaacttatcg ccaaccagca acctgctttt cgcgtggtac tgggtgcctg gcatacggaa
     4681 ggttcgatgg tgaaagtcac ggcggatgac gttgagctga ttcattttcc gttttaaaaa
     4741 acccgcaact ttgctgattt cacagccacg caaccgtttt ccttgctctc tttccgtgct
     4801 attctctgtg ccctctaaag ccgagagttg tgcaccacag gagttttaag acgcatgtct
     4861 tcccgcaata atccggcgcg tgtcgccatc gtgatggggt ccaaaagcga ctgggccacc
     4921 atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc cgcaccacgt tgaagtggtt
     4981 tctgctcacc gcacccccga taaactattc agcttcgccg aaagtgccga agagaacggt
     5041 tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc tgccgggtat gattgccgcc
     5101 aaaacgctgg tgccagtgct gggcgtgcca gtacagagcg ccgcactgag cggtgtcgat
     5161 agcctctact ccatcgtaca aatgccgcgc ggcattccgg tgggtacgct ggcgattggt
     5221 aaagctggcg cggcaaacgc ggcgttactg gcagcacaaa ttcttgcgac tcatgataaa
     5281 gaactacacc agcgtctgaa tgactggcgc aaagcccaga ccgacgaagt gctggaaaac
     5341 ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct cggtaacggg cagttaggcc
     5401 gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt ctggccagtc gggctggacg
     5461 ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac cgctgagatc gaacgctggc
     5521 cggaaaccgc attaacccgc gagctggcgc gccatccggc ctttgtgaac cgcgatgtgt
     5581 tcccgattat tgctgaccgt ctgactcaga agcagctttt cgataagctc cacctgccga
     5641 ctgcaccgtg gcagttactt gccgaacgca gcgagtggcc tgcggtgttt gatcgtttag
     5701 gtgagctggc gattgttaag cgtcgcactg gtggctatga cggacgcggt caatggcgtt
     5761 tacgcgccga tgaaaccgag cagttaccgg ctgaatgcta cggcgaatgt attgtcgagc
     5821 agggcataaa cttctctggt gaagtgtcgc tggttggcgc gcgcggcttt gatggcagca
     5881 ccgtgtttta tccgctgacg cataacctgc atcaggacgg tattttgcgc accagcgtcg
     5941 ctttcccgca ggccaatgcg cagcagcaag cgcaagccga agagatgctt tcggcgatta
     6001 tgcaggagct gggctatgta ggtgtgatgg caatggagtg ttttgtcacc ccgcaaggtc
     6061 tgttgatcaa cgaactggca ccgcgtgtgc ataacagcgg tcactggaca caaaacggtg
     6121 ccagcatcag ccagtttgag ctgcatctgc gggcgattac cgctctgccg ttaccgcaac
     6181 cagtggtgaa taatccgtcg gtgatgatca atctgattgg tagcgatgtg aattatgact
     6241 ggctgaaact gccgctggtg catctgcact ggtacgacaa agaagtccgt ccgggccgta
     6301 aagtggggca tctgaatttg accgacagcg acacatcgcg tctgtccgcg acgctggaag
     6361 ccttaatccc gctgctgccg ccggagtatg ccagcggcgt gatgtgggca cagagtaagt
     6421 tcagttaagc aggtgctcat ttgattgccg gatgcggcgt gaacgcctta tccgacttac
     6481 cgctccggta cccattgtag gcctgataag acgcgtcaag cgtcgcatca ggcatggtgc
     6541 gccgattgcc ggatgctctc atcactacag cgaaatacag gtccccgctt ctcccgccag
     6601 cgtctcttca attcgcgata acgccccaat ccacgcgggt ttaccacggc ttctgacata
     6661 accactcacc gccgttacct tcggccccat cgaaccatcg gctttggcaa atggcgctaa
     6721 ctcatccggt gtggcatggc gaatggcgcg ttgctgcggc gttccccagt tttcatatac
     6781 cgcatcagca tcggtgagga tcaccagtcc atctgcatta atctgctcgg cgagcaacgc
     6841 agcggcgaga tctttatcaa tcactgcttc actccctgct ccgtcatccg tcacaggcac
     6901 accgccgccg ccactgcaaa tcaccacatg cccctctttg agcaacaact cgatggcttc
     6961 gctgtcgaga attttacgcg gttgcggaga cgccaccacc cggcgtaaat atttaccatc
     7021 acgtttcatc tgccagccgt aagccgcttc cagtgcctct tgttcttctg gctgataaac
     7081 cggaccaata aatttctctg gctgcaaaaa cgccggctca tcaggcgaaa cctcaatgcg
     7141 cgtcagcacc gtcgtcacgg gcggtatctg cggctgtgcg ctcaaactct gcgccagcat
     7201 atagccaatc atcccctgac tttctgccac cagcacatcc agcggatacg gttctacctc
     7261 tttccacgcc agattctgca atgccagcag ccccacctgc ggcccgttgc cgtgaacaat
     7321 cgccagccga taagaacggg ccaggcgcgc cagcgcgggt acagcactgg cgatattgcg
     7381 atattgattt tctgccgtca gcgcctcacc gcgctgaagc aaggcattgc ccccaagagc
     7441 tacaaccagt gttttcatgg ttttccttgt aataattgtt gaccaagcca gaaccccagc
     7501 agtgtatctg cccctgacgt atgccccagc gccagcagcg aatcaatcgc aacagcggta
     7561 cgtttcgggc aactcagagc atgaacaaag tgcaggagtg gcgaggcgaa atatccttgc
     7621 gcggcataac gtaaataact gacgctcacc gctgtggtaa cgagttgcag attgtcggaa
     7681 caggcaaaaa acggacgacc ggagcgcgca tctaaagcgc cataatacca ggccgccagc
     7741 agcattccgc tcagcgtgtc atcatgactc ggcgttaatc cggggccttt acccagccag
     7801 tgccgccagt cggtcttaac gccattgagc gcggcctgaa aacagtgacg aaactggcgt
     7861 aactcagcag gcagcggatc gcttgccgcc aacgccagtg gcccgaaaag cccggtttcc
     7921 tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg cgtaatacgc
     7981 agcaaacaat aacgctgtgg ctgtttaacc gtaaaacgcc cgaggcgaat cccttgagcc
     8041 acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa ttgagcgcgg
     8101 cgaagcaccc atcctccagg gccgaaacca ctaccctgac gatgcagcgt taacagttcg
     8161 ccgctttccg tcatcaggtt aatcgcccgc cgccacactc ccgctaaccg ccaggactgg
     8221 cgataattcg gtgcgctact actggcaagc agaggatgga tgatcgtcat tgcgcgttcc
     8281 tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaagc acgccagcgg
     8341 tgcccgcacg gtgcctgcgc caatctgccc gatccccgcc tctttatggg cgataccggt
     8401 attgatgagt ggcgtaatgc cggtttctac cacgcgacga atatccagcc ccaggcacgc
     8461 gccctgaaaa tcccagcttg ggatctgcaa ctgcatattg cgttcaaggt aaatttccgc
     8521 catctcttca gataccgccc tggccgcttc catgccaccc gcaccgacaa agcgcgttac
     8581 gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa tcgcgctatc
     8641 gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac cttgcggagt
     8701 gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc cgaacatatt
     8761 gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc cagcatccat
     8821 cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca cgctgaggaa
     8881 atccatcact tccgcaatgt gctgtttatc atgatcgagg cgagcaattt gtggggccag
     8941 cgtacgcatt aacagtgctg aggaagcaat attgcgttga tggaactcat cgcccatcgt
     9001 aatgccctgc gccatcattg ccgtgagatc gataccgcgc tccatgcgcc ccagcgccgc
     9061 gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga catcttcgcc
     9121 gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt acgcccggtt
     9181 gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa taccgcccat
     9241 tggcccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct gctccagtat
     9301 tgccagcgcc tgcgcttcat ctttcgccca accttcgaac agacatgcgc ccacgcacgc
     9361 ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt gaagcagtgt
     9421 tttgccctgg tttagttcgc tgataagtga agaagccggt tgcacatcca gccagtgtgg
     9481 acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg atgtaaacat
     9541 gggaacccct tattgcaaac gttctaataa acgagccagt tttttattgc cgccggcgac
     9601 tggcgaccat tggtaatgca caaccggttt gctggcgctt tgtagctcca gcgcaaaact
     9661 gcgtaatccg atgttaatca cggcgacgtt ttccagtaat gacggtgtgt gttgctgtgc
     9721 ggcaggcgag agcggatgaa ttaacgctgc cgccagcaag gtggcttccg gtagcgaact
     9781 cacgaccgca atccccgcat cttccagctt ggcgatttgc tgcgagcggc attgcgggtc
     9841 acgttcagtg cctgtcaccg tggcaatggc atacagtggt tgattgtctg aacgcgcagc
     9901 acaggctttt tgccaggcgc ttaccagcga ggcggcagga tcggcggtcg caccgaagcc
     9961 aatcacgaca tcaagcagca gcacacgcac ttgcggttta gcgccgagat cggtaattaa
    10021 ctggttgcgt aaggttgggt cgatcatcgg atggggacgc ccgacggtgt agaaatcatc
    10081 gccgaggtca ataatctggt gaccatcggc gtcgagcatc atgccgtgat gatgggcatc
    10141 atcggcttcc acgccaaggt gtccggcaag taatcccgcc gcttcggcag ccagcgtacc
    10201 gccggtatac aaaccgcaaa taaatccgct gctggcaggc gttatcgcgt tacgtcgcgc
    10261 cgtaacgcgt gaaagcaggc aagccaggcg tgcggcctca tccagcgagg aggcaaacca
    10321 gacattctcg tcgcgggcca ccgccggggt ataacctaaa aacagcgcca ccgtcggttt
    10381 gccggttgct ttcatggcat taacaatttt cagacgcaca gcatcggcag gtggttttga
    10441 aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc atttccagcg ctgttagcgc
    10501 actgatgccg cccacttcac ggctgaggtc gcgcccgcca aggccaatcg cgtgagtaat
    10561 tccctcccct gccagcgcaa tctgcgaaca cagctcctga atcccggtac cggaagcgcc
    10621 aatgacgcca atattgcctt ccggcatcac gttagcaaaa gccagcggcg tggcggcaat
    10681 catcgacgta ccgcagtctg gccccatcac cagcaagcct ttttcccgcg cgcgggtttt
    10741 aagttggatt tcatcttcca gcgtaacgtt atcggagaac atcatcacgt tgaggttgcg
    10801 atccagcgcc tgattcgcca gctccgccgc atactcgcca gccactgaaa tcagcgccag
    10861 attggcatcg ggtaatttct gacaggcact gtcccagcga cgcacctgcg tcaacgcctg
    10921 gctgctgcct gacccctgcg ccagttgttt tagcgcctct tcaagctgct gcataatcgc
    10981 ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt
    11041 ggcgttatta aaatcgtcat gccagaaacc tgtggtatct aataacgctt tattggcggg
    11101 cgtacccatc attacggaaa catcatcaac attttctgat tcgctgagtt ttcgtgaaat
    11161 aatcattaaa ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt ggatcataac
    11221 aactccttaa aaattgcacc catggcaatt gcacgagcag tgttaatgtg gcgattacgg
    11281 tatcgatgac aacgagtaaa taaggtgatg aatatcacac agggattatt aacaaaa
//
LOCUS       DAZUGK010000145        11198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZUGK010000145 DAZUGK010000000
VERSION     DAZUGK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.146"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(56..514)
                     /gene="yobD"
                     /locus_tag="ACHY5W_003398"
     CDS             complement(56..514)
                     /gene="yobD"
                     /locus_tag="ACHY5W_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFS4448242.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(569..1420)
                     /gene="manZ"
                     /locus_tag="ACHY5W_003399"
     CDS             complement(569..1420)
                     /gene="manZ"
                     /locus_tag="ACHY5W_003399"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFS4448243.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1433..2233)
                     /gene="manY"
                     /locus_tag="ACHY5W_003400"
     CDS             complement(1433..2233)
                     /gene="manY"
                     /locus_tag="ACHY5W_003400"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFS4448244.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2296..3267)
                     /gene="manX"
                     /locus_tag="ACHY5W_003401"
     CDS             complement(2296..3267)
                     /gene="manX"
                     /locus_tag="ACHY5W_003401"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFS4448245.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YIVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3584..3742
                     /gene="yoaL"
                     /locus_tag="ACHY5W_003402"
     CDS             3584..3742
                     /gene="yoaL"
                     /locus_tag="ACHY5W_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFS4448246.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3730..5286
                     /gene="yoaE"
                     /locus_tag="ACHY5W_003403"
     CDS             3730..5286
                     /gene="yoaE"
                     /locus_tag="ACHY5W_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFS4448247.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5290..6888)
                     /gene="pdeD"
                     /locus_tag="ACHY5W_003404"
     CDS             complement(5290..6888)
                     /gene="pdeD"
                     /locus_tag="ACHY5W_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4448248.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(7019..8383)
                     /gene="sdaA"
                     /locus_tag="ACHY5W_003405"
     CDS             complement(7019..8383)
                     /gene="sdaA"
                     /locus_tag="ACHY5W_003405"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS4448249.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8567..9145)
                     /gene="nudL"
                     /locus_tag="ACHY5W_003406"
     CDS             complement(8567..9145)
                     /gene="nudL"
                     /locus_tag="ACHY5W_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFS4448250.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(9149..10510)
                     /gene="pabB"
                     /locus_tag="ACHY5W_003407"
     CDS             complement(9149..10510)
                     /gene="pabB"
                     /locus_tag="ACHY5W_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFS4448251.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10584..10763
                     /gene="yoaH"
                     /locus_tag="ACHY5W_003408"
     CDS             10584..10763
                     /gene="yoaH"
                     /locus_tag="ACHY5W_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFS4448252.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(10883..11181)
                     /gene="yoaC"
                     /locus_tag="ACHY5W_003409"
                     /pseudo
     CDS             complement(10883..11181)
                     /gene="yoaC"
                     /locus_tag="ACHY5W_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
BASE COUNT         2798 a         2834 c         2831 g         2735 t
ORIGIN      
        1 agctatgtct gattattaaa aatacactaa acatagcttt ggctaaattc ataagttatt
       61 gagttgatac gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta
      121 acagccgacg ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt
      181 tgattcggct atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga
      241 tcttcggaac gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc
      301 atgttgttat tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc
      361 cgacgaagat aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg
      421 ggccgttacg gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga
      481 tgaaaagaat cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc
      541 caaaaggccc cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag
      601 cgataccgat gacgaagaag ccaacgatga tccacagcgg attaactttt ttgcgcagca
      661 gccacataca agcaaaggtc agcagcagtg gtaccaggcc tggcattaac tggtccagaa
      721 tagtctggac agtagtaacg tgttctttgc ccgtctggtc agtaatgcga gagacaacca
      781 gcgggatgtt gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga
      841 tagacgcccc ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga
      901 tacctttgga gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa
      961 acaggatgaa gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca
     1021 gtgctgcaaa taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg
     1081 gccccatcaa accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag
     1141 cacgctgttc ttccagcgcc agggttacgc cgagaatcgg cgcagccacg aacggctggg
     1201 tgttaaagaa ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt
     1261 agaggcgacg aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga
     1321 agttccatga accctggaag aggttagaac gcaggaagac gccacgaata tcactttgag
     1381 tgagtttttt ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca
     1441 gttcgttatc gagatcgttg ttaccagcag cctgagcagg cgcaccggct acgcggttgt
     1501 atttcgggct aagttggatg tagagcactg ccataacagt accgatcaca cccagagcaa
     1561 ccaggttaaa gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt
     1621 agccagcacg catcatgttg ataaccatcg cataaccaac caccacgatc atgccaccgg
     1681 cgatattcag accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc
     1741 tggtacctac agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga
     1801 acagagaaga aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat
     1861 ccgcagcgtg ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg
     1921 cagcggccag agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa
     1981 ccagaatggt agaaatgata gatgccagag cggcgtcagg cgcaactgca gcaccgatgt
     2041 tcatccagcc cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat
     2101 ccccaagaac gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat
     2161 cgaggattga tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag
     2221 tggtaatctc cattgtactt ctcctgttta cgtaagacta aagtgtgaaa actgagtgat
     2281 aatcaacaca atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg
     2341 atcggtggaa accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc
     2401 ctcgatatct ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa
     2461 tgccatacca ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac
     2521 atctgttggg ttggtaaata acagcattac acgttcgcca gcatatttcg ggttgttgta
     2581 gacgcgaatc attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg
     2641 ggtgagcagt gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg
     2701 ggagacattg gtttctttag tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc
     2761 gatacgcgca aggccaataa ctatgtagtc gtttggtccc attggtttgg ccggagttgg
     2821 agccgctttt ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt
     2881 cagtgctttc acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct
     2941 tgggtcatca tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cacctgcaat
     3001 gacttcataa tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc
     3061 ccatgtatca acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt
     3121 gtacttttca atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt
     3181 ttcctgctcg cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg
     3241 tgtgcctata acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa
     3301 cgtgccagat gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca
     3361 accaaaaacc cctgcgattc gcgatttatt ttagatatcg aaaaaattat tttatgtgat
     3421 gaagatccgt aatttaactt tcgattagca gaaatttcga aaggtaaaat atccttgtca
     3481 cattcgtttg caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgttaaa
     3541 ctcccgttta acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg
     3601 tcatttcagc atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg
     3661 tttgcatttt gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag
     3721 gagcttgtca tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt
     3781 gttctcgaaa ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa
     3841 ctgccgccaa aacaacgcga taaagcgcgt ttactggggt tatcactggc gctgattatg
     3901 cgtctggggc tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc
     3961 gtcatggatt tctccttctc cggacgcgac ctgattatgt tgttcggggg gatattcttg
     4021 ctgttcaaag caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc
     4081 cacggtaaag gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc
     4141 gtcttctcgt tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg
     4201 atggcggcgg tagtgattgc gatggcggtt atgttgctgg catccaaacc gctgacgcga
     4261 tttgttaacc agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt
     4321 ctgagtctgg tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg
     4381 attggcttct cgatcatcat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt
     4441 caccagtcga ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc
     4501 gggaaacgtc aggccaatgt tcagcacgat gccgataacc cgatgccgat gccgatcccg
     4561 gaaggtgcat ttgccgaaga agaacgttac atgattaacg gcgtactgac gctggcgtcg
     4621 cgttctctgc gcgggatcat gacgccgcgc ggtgaaataa gctgggttga cgctaatctc
     4681 ggggtcgatg aaatccgcga gcagctgctc tcctcaccgc acagcctgtt cccggtatgt
     4741 cgcggtgaac tggatgaaat catcggaatc gtacgtgcta aagaactgct ggtggcgctg
     4801 gaagagggcg ttgatgtggc ggcgattgct tcggcgtctc cggcgattat cgtcccggaa
     4861 accctcgatc cgatcaacct gttgggcgtg ctgcgtcgtg ctcgcgggag ctttgttatc
     4921 gtgaccaacg agtttggtgt ggtacaaggt ctggtcacgc cgctggatgt gctggaagcc
     4981 attgcgggtg aattcccgga cgctgacgaa acgccggaaa tcattactga tggtgacggc
     5041 tggctggtaa aaggcggtac agatttgcat gccttgcagc aggcgcttga tgttgagcac
     5101 cttgccgatg acgatgatat cgcgacggtc gcgggcctcg tgatctcggc aaatggtcac
     5161 attccccgtg tgggcgatgt gattgatgta gggccactgc atatcaccat cattgaagcc
     5221 aatgattatc gtgttgatct ggttcgcatt gttaaagagc aaccggcgca cgatgaagat
     5281 gagtaagcgt taacgtaacg gcataatggg cgtgatatgt ccattatgcc gggcgggcgg
     5341 cggttggctg cccgccagcc atttggggaa atcacgtagc ggcatcggct gtgcgtacaa
     5401 atacccttgc aaaatatgca cgccatgacg gcgcaaatac ttcgcctgtt cttgcgtttc
     5461 cacgccctcc gccaccagtt caatattcag tctttgcccc agcgcgatga tgatatcggt
     5521 caccgtcgag ttaaccgcgt cagaacctat agctgcggta aatgacttat caattttcag
     5581 cacgtcagga cgtaatgttt caagccagga aaatgaactg ttgccggtgc cgaagtcatc
     5641 aatcgccagt ttgacgtttt tacgatgtag ctcgcgggca atccgataat caacatccag
     5701 taaggcatcg cgttcggtga tttcgaggat cagttgctga atcgggtgag cgctaaacca
     5761 gtactgattg agatctttta tcagcacacc acggcgaaaa tggctgggtg cgacgttaat
     5821 gccaacatga aactgactac tcatcgggaa aacatggcgc tgacgaatgg tttctgccat
     5881 cacatagcgg gtcagtggca caattaaatg atgttcttcc gcgataggaa taaacacatc
     5941 cggtgaaatc cagccctgac gcggattgtt ccagcgcagc aaaatctcta caccaataca
     6001 ttgctggctg cgcgcattaa gcaaaggctg acagaacaat tcgaactcat gttgcgccag
     6061 gcccagattg atttcgcggg aaaagctcat ccggtaagcg gtcgccagcc aggcgatgta
     6121 gcccaccagt aaacttagca gcaccgccag cggtaattgt gttggaagat agtgccatgc
     6181 cagcgccgta gcccccggac cattaacgtt aatggtaaaa ggaaagcgtt gcgaagaaac
     6241 ctgatagttt tcattgtctt caggttgcgg aagggaatct accagcccat taccatagag
     6301 caaatgccgt ttgtccaccg tcaggctggc gctactgatt tgcggcagtt gtggctcaag
     6361 gagcattgcc gtcagtaagt cgatgttaat catctccatg accccagcat tgctactgct
     6421 cgctgcaggc gtccattgaa tcaaaaccgg actgccttta atcaggacac ggtcggtcgt
     6481 caggcgtaaa agtggttgcg gtgcaggaag ttcagccaga atgtccacga cgggcacatt
     6541 gcgataacca aaaatgctgg agcaataaag tgtgccgtct tgcaccaggc caatggatcg
     6601 cacagtttgt agttttgccg cctgtttacg taatggcaaa tgggcgacag agcagggcag
     6661 accaatcagc ggaagcaaga cttcgctacc tgcctgtagg ggaagcagta ctttatctaa
     6721 ttcctctaca gcgtgattgg cgaattgtac tacccgttgt tgatttaagt tacgctgtga
     6781 aataaatcgc acgctcaggg tcgaagcgag cgtaacgagt gcgcaaatcg tacaaacaat
     6841 cattcgatta cggcgatagg ttttaatgat ccgttgtgct ttttgcatgc gggccaccct
     6901 gtaagcgtat ggcgacaaac gcctgacgca acagtggaag tgtagtgggg aatattacag
     6961 cagacgagaa agcggggata aattcgccca tccgttgcag atgggcgagt aagaagtatt
     7021 agtcacactg gactttgatt gccagaccac cgcgtgaggt ctcgcggtat ttggcgttca
     7081 tgtccttacc ggtttcgtac atcgtttcga tgaccttatc cagcgagacg cgcggtgcac
     7141 tggtgcggcg cagagccatc cgtgcggcgt taatcgcctt cacagaggca atggcattac
     7201 gctcaatgca cggcacctga acctgccctg caaccgggtc gcaggttaaa ccaaggttgt
     7261 gttccatgcc aatttccgcc gccacgcaaa cctgttccgg gctaccgccc agcagttctg
     7321 caagacccgc agcagccatt gaacaggcaa cacccacttc gccctggcaa ccaacttccg
     7381 caccggaaat agaggcgttc attttataca atgcaccaat cgcgcccgct gccataaagt
     7441 aacgggtata gatgtccggg ctgaccgatt caataaagtg gtcatagtaa gccagcactg
     7501 ccggaacgat accgcaggca ccgttggttg gcgcagttac cacacgacca ccggcggcgt
     7561 tttcttcgtt aactgccagc gcaaacatgt ttacccagtc aatgacattc atcggatcgt
     7621 tagacagttt atcgctggaa accagcatcc ggcgcagggc agacgcacga cgcggcacgc
     7681 gcagcgggcc tggcagtaca ccttcggtgt tcatcccgcg atcgatacat gcctgcatgg
     7741 tttgccagac atgcgcgaaa tactcgtcga tctctttctt gctgtgcagc gccagttcgt
     7801 tctgcatagc gagaccagac agcgaatagc cggtttcatt acagtacgcg agcagttcgg
     7861 tggcagattt gaacggatac ggcacgctca cttcgttggc agcatcctga ccaaagtgtt
     7921 cttcatcgac gataaaaccg ccgccgatgg aataataagt tttgctgtag acgacttcat
     7981 cgccgttata ggcgtggatt tgcataccgt tttcatgcag cggcaggttg ccgttatgaa
     8041 aacgcatccc gttgtcgcgc gggaaatcca cttcatgccg tccctgtgcc agcagcagac
     8101 gttcgcgctc ttctacgtcg cgaataaaac cgggaatact gtcgatatcc acggtggcag
     8161 gttcgttacc tgcaagaccc ataataatgg cgatatcggt gtggtggcct ttacccgtca
     8221 gcgacagtga accataaacg tccacggcaa cgcgagtaac gctatccagt aagccttttt
     8281 cgaccagatc atcgacgaac tgtttacccg ccttcatagg ccctacggta tgggaagatg
     8341 agggaccaat ccccacctta aacatgtcga atagactaat cacgataata ctcctgacaa
     8401 ggtgactgga cttccagtaa cgaactaata acagcgcata gtgtaagagg gaacgcggcg
     8461 actggcttaa ctattcacat gaattaaact aatcaaaaaa cgcgatttaa ctaatattgc
     8521 gatgattgtc tcaagtagtg ttttgtagat gtaaagataa gtatagtcag ggtttcacac
     8581 caatttgcag cgccagctca cgaattatgc ctgcggtcat tccccataca aaatactgtt
     8641 cgtaccagga cagccatacc cgatgtgaat cgccacggcg gtagatatct aaagggtgat
     8701 aacgacccag atgtaatgcc tgggcgagcg gcatttcaaa caccgccgag acttcatctt
     8761 cactggcgcg atacggcaga tcgggcggga taatgccgac cactggggtt acctgatagc
     8821 cagttacgct atcgacgggc ggcagcacgc cgataacttc aacggcggaa ggcggtatag
     8881 caacctcttc ttcagcttcg cgcagcgcgg cggcgataac tgatgcgtcc gtgtcatcga
     8941 ctgcacctcc agggaatgcc acttgtccag cgtgtttacg cagatgaatt gaacgctgag
     9001 tcagcaacaa ccccggttgc ggtcgacgga cgatggggat taacacggca gcctgacgat
     9061 gatttagggt ttcccggtta atttgtgggc gcaaaagttg aaagcgcgat aaaaaatcat
     9121 caagcgtcag gctacggtat tccacgtctt acttctccag ttggcgtaat atcttattaa
     9181 ctttatcaaa agtttcctga tattccgctt cttcctggct atcggcgaca attccaccgc
     9241 ccgcagagca gtatatttgt ccgttaatgg cagtcagcgt gcggatagta atgctggtat
     9301 ccatgttgcc gcaaaagctc aaatagccaa tgctgccgca ccaggcatta cgtcgctgcg
     9361 gttccagttc gtcgataatt tccatagccc gtactttcgg agccccggtt attgagccac
     9421 caggaaaagc tgcgcgcagc agatcgctgg cgtgtaactg ttctggtagt cgcgccgtta
     9481 tggtgctgac cagatgatgc acggcaggga agggttccac cacgaacagc tctggtactt
     9541 ttaccgaacc tgctacggca acacgaccga tatcattacg cattaaatcg acaatcatca
     9601 gattttcggc acgatctttc gctgagttcg ccagtttttc tgcttgtttg ctatcctcct
     9661 gaggatcggg caggcgtggt agcgtgcctt taatcgggcg ggtctggatt tcactattat
     9721 cacaaagaat aaaccgctct ggcgaaaggc ttaaaattgc accctgttca agacgtaaaa
     9781 aagcgctaaa tggcgcgcgg ttggcctgat taagctgaag gaatgcctgc cattcatcgc
     9841 cagaataggt cgcatgaaaa cgctgggcga gattcacctg atagcaatca ccgctgtgca
     9901 gatattcctg tacttggcga aatttttcgc cgtactgctc gcgggtcata ttggattgcc
     9961 agtcggaagt gagcgtgaaa tcttcctgcg gcgagaattg ctggctttca agccaggccc
    10021 gacgggcatt gacatcatta tgactcagca aagaaactgt atgacgctgg tggtcgacaa
    10081 tgagcgccca atcgtagata cccactgcca tatccggcag aacgatatct tgttccgcaa
    10141 tttctggcag tgactcaaaa cggcggccca gatcgtagcc aaacaacccc agtgcgccgc
    10201 cctgaaatgg caaatcttcg ttatgcgttg ggcgaatgtc tgcgcgatcc agcacctgct
    10261 ggagcacctg tagcgggtca tcagtggtcg ttgtgcgttt ttcgctttca ctaacaacgg
    10321 tttctttacc gaaagtggtt aaagtgcaaa tcggctcggc gaccacaata tcaaagcggc
    10381 tatacggatg atcggcatag ccggagtgta aaagcatcgc ccacggcagg tggcttaagc
    10441 gggagaaata aaattcagcg gcgtcctgac gccagggtaa agtaatcaca gcgggagata
    10501 acgtcttcat cagtcctgac tctactggct atgtgccggg tagcatgaaa taattagcgc
    10561 caacaattta gcaggagtta acaatgtttg caggtttacc ttcactcacc catgaacagc
    10621 agcaaaaagc tgtcgagcgg atccaggaac tgatggcaca agggatgagc agcggccagg
    10681 caatcgcgct ggtggcagaa gagctgcgtg ccaaccactc cggcgagcga atcgtggcgc
    10741 gttttgagga tgaagacgag taacacacta atccgtatcg atttcgttat agaagccgat
    10801 gattatttga ttgtgagaaa aaggacgctt tttatatgtg attaaaagcg tcctgaccca
    10861 tttatgctga tataaattta actcagacca gtgcttttag ttcttcctgc atatatgttg
    10921 aaaagtactc aggattttta atcacagagc gttcacctgt ctccattttt ttgcccatcc
    10981 aaccattttt tccgtgaacc cagggttcca gccttcctgg tcagctcgcg ccgtaatatc
    11041 agcggcactc gcgggtactc ccagttcttt taaatattta aagattccct tcgccgtgct
    11101 ttcattcatc gaacttggtg ttggcgctga tacatccatg gcaccaatga aatccagggc
    11161 tttatctatt acagcaggca tattcttatc cttaaaat
//
LOCUS       DAZUGK010000146        11147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZUGK010000146 DAZUGK010000000
VERSION     DAZUGK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.147"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1307)
                     /gene="yeeO"
                     /locus_tag="ACHY5W_003410"
     CDS             complement(<1..1307)
                     /gene="yeeO"
                     /locus_tag="ACHY5W_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFS4448253.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVV"
     gene            1468..1543
                     /locus_tag="ACHY5W_003411"
     tRNA            1468..1543
                     /locus_tag="ACHY5W_003411"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1501..1503,aa:Asn,seq:gtt)
     gene            complement(1581..2531)
                     /gene="cbl"
                     /locus_tag="ACHY5W_003412"
     CDS             complement(1581..2531)
                     /gene="cbl"
                     /locus_tag="ACHY5W_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFS4448254.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2633..3550)
                     /gene="nac"
                     /locus_tag="ACHY5W_003413"
     CDS             complement(2633..3550)
                     /gene="nac"
                     /locus_tag="ACHY5W_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFS4448255.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            3877..3952
                     /locus_tag="ACHY5W_003414"
     tRNA            3877..3952
                     /locus_tag="ACHY5W_003414"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Asn,seq:gtt)
     gene            complement(4008..4943)
                     /gene="ldtA"
                     /locus_tag="ACHY5W_003415"
     CDS             complement(4008..4943)
                     /gene="ldtA"
                     /locus_tag="ACHY5W_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS4448256.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5005..6084)
                     /gene="cobT"
                     /locus_tag="ACHY5W_003416"
     CDS             complement(5005..6084)
                     /gene="cobT"
                     /locus_tag="ACHY5W_003416"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFS4448257.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6096..6839)
                     /gene="cobS"
                     /locus_tag="ACHY5W_003417"
     CDS             complement(6096..6839)
                     /gene="cobS"
                     /locus_tag="ACHY5W_003417"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFS4448258.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6836..7381)
                     /gene="cobU"
                     /locus_tag="ACHY5W_003418"
     CDS             complement(6836..7381)
                     /gene="cobU"
                     /locus_tag="ACHY5W_003418"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFS4448259.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7533..7706)
                     /locus_tag="ACHY5W_003419"
                     /pseudo
     CDS             complement(7533..7706)
                     /locus_tag="ACHY5W_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7685..7873)
                     /locus_tag="ACHY5W_003420"
                     /pseudo
     CDS             complement(7685..7873)
                     /locus_tag="ACHY5W_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8696..9226)
                     /locus_tag="ACHY5W_003421"
                     /pseudo
     CDS             complement(8696..9226)
                     /locus_tag="ACHY5W_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(9276..9623)
                     /gene="tnpB"
                     /locus_tag="ACHY5W_003422"
     CDS             complement(9276..9623)
                     /gene="tnpB"
                     /locus_tag="ACHY5W_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4448260.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            complement(9620..10003)
                     /locus_tag="ACHY5W_003423"
     CDS             complement(9620..10003)
                     /locus_tag="ACHY5W_003423"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4448261.1"
                     /translation="MRAKERLPRKHYSPEFKMELVRLALEEEGSIAALARKHDVNDNL
                     LFKWIRLWQREGRVCRPRKNSSSLPALIPVQLQAGSSLPTFELPSCSPPATCHIKCRG
                     GEITLTHPSAELMSTVLRELMRGPV"
     gene            10082..10241
                     /locus_tag="ACHY5W_003424"
                     /pseudo
     CDS             10082..10241
                     /locus_tag="ACHY5W_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            10285..10440
                     /locus_tag="ACHY5W_003425"
                     /pseudo
     CDS             10285..10440
                     /locus_tag="ACHY5W_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2998 a         2948 c         2550 g         2651 t
ORIGIN      
        1 actacaaccc ggcaacccca catgctcaac atagaaaccc acatggcgta acgtgcatcg
       61 cgagcaccct taaatccagc gggtagcacc catgaggcag accaaatagg cataaataaa
      121 gcatttagcc aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac
      181 gatgccataa ccccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg
      241 gaaagccaga acacatgccg caactgaatc tctgcttgcg ctatctgtcc tacacccaac
      301 cttcggcctg taatgatcgt agaagcagag ccgagcgcac ttccgggtaa gttgataaga
      361 gccgcaattg aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg
      421 aacatttggg ttaataaccg accactggta aataacactg attcgacact cgcgggaata
      481 ccaatcccca tgacttccca gataatgcta aaattcagcg gtttaaaata gctctttaac
      541 gaaatcctta gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc cactgcgcca
      601 atataacgag aaatggttaa ccccaaccca gccccgacaa atcccagtcc cggccaggag
      661 aaaaggccgt aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta
      721 atcagtagcg gtattttcgt attccctgca ccacgaagtg ccccgctacc aatcaaagtg
      781 atggcagctg ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta
      841 acttctgtcg tggcatcacc cgcgacgaaa tcaataattt gctcgccaaa atgatgaata
      901 agcgttgcca acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc
      961 ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga
     1021 tcgatagcag caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg
     1081 gccatcgcat cttttcccag ccagctgacc agaaaagtgc tcagaacccc catcaacagg
     1141 acacaggcat tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac
     1201 actttgtagc tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca
     1261 gaggagatat tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag
     1321 ggagaatcag caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact
     1381 gttgatagaa acggcaaaca gttgggaaaa ttaaaaatcg ggtttgacaa aagatttttc
     1441 gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact
     1501 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg
     1561 cttttatagc gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc
     1621 acctgccgct tgatatcctc aactgaaagt cctgcattac aaagttccag aaagcgccag
     1681 acatagttac gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcaaaa
     1741 agatgccgcg tatccaggcg gattaaattc ttttcctctt gttcaccgct ggattgttcg
     1801 gcaactattc cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgc
     1861 gcacttaata caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag
     1921 cgccccgtaa tcccctgtcg gtaagtgatt aacggccact tcgctattga ttccagcgtc
     1981 aatggtgtaa tttgcgtcaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga
     2041 aaccacggga aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata
     2101 tcagcttcac cattatgcaa caacgtcgca atttcctgtg gcgtcccctg gattagctcg
     2161 agccgaacct ccgggaaaag ttcgcgaaaa gatttaatga cctctggcaa actataacgt
     2221 gcctgagtat gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct
     2281 gcaagccgac gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct
     2341 ttgcccggtt cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca
     2401 agttcatcct ccagttcccg aatatgacgg ctgacgccgg actgtgaggt aaaaagcata
     2461 ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt
     2521 tggaaattca cggtaaactc cgggcagttc agattttccg ttattgttaa agtctaatgc
     2581 ccggcataac aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca
     2641 ccaattgcca ctgccttttt tccatcactg gagaactcat cactgacatt aacaactctt
     2701 tcactgcctg tgcctgtggt gataagttcg ctctggcggg taaatttaat gacaaagaga
     2761 gactcatgga aggtgtggta atgcgtgaca tccacccgtt tactgcgcca cataacgaac
     2821 gcgcggccga ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg
     2881 tggcaataga ctcaatttca ccaatgactt ttgccgtgag tcgccgtagg gaaaaagcct
     2941 catcaacacg aagtctaaca gcactgtaat cactgggaag aaagaggttc atttgcgcaa
     3001 tagcattaac atcaacgctt tgccccggac aatcttgagt ccctaccagg aaaagatctt
     3061 ctttcagtaa agcctgactg gatacaccag ccacagggga atgctcataa atcaccgcca
     3121 tatcgagttg gtgatttatc agtttttcgt taagcactgc accgctattt tcatgaagat
     3181 agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg gtgatggatg
     3241 acgcagcggt tcctggcgca aagccaatcg agacttgccc cgataatgcc tgaccaacgt
     3301 tatgcaccgc cagttgggcc tgctcacact gacgtaaaat ggcccgcgca tgggtataga
     3361 gaatttttcc ggcgtcagtt ggcgtaacgc cccgttttgt gcgaatcaaa agttgttgat
     3421 taaactcacc ttccagtgtg gcaacctgct ggctgagcgc tggttgtgcg atatgcaata
     3481 cttcagcagc ctgggtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc
     3541 tgaagttcat gttgcctccg gtttttaaga gtcggcccaa gtgccgccat tacttacaac
     3601 cagaatgcaa gatgcttgcc agttctattt tggcgttgat gcgcaagcta accaactgtc
     3661 aaataagaga ttatgataga ttcgtcattg gctcttttta tctgcagtcg cattcacctg
     3721 ccctataaaa ggagggtatg caccacgatg gttcattacc caataagatt gaaagctcac
     3781 cactttgttg aaattggcag caaacaaaca aaaaaatgca tttcaccctt tgacatcacc
     3841 atgcactgcc attaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa
     3901 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcaaa
     3961 aaagcctgct ttcgagcagg ctttttactt tctaattatc aacgctctta aaacatctgt
     4021 cttgaaccag aactaatttg cacaggcatt cccgatcgac gttgcaacgc agcatttgcg
     4081 cgatttacat caacttcttg cccgttgata aacgcccgca aagatggggt taccggcaat
     4141 ggcacttttc ggtcagactc atattctgca cgattgcgcg acagcggctc atgaacttcc
     4201 agccactttg aaccatccgg ttccgttgtg tatttcactg gctgatcaat aatttgtaca
     4261 cgagtgccta caggaacatt atcaaacaga tatttgatat cgtcattacg cagacgaata
     4321 cagccctgac ttacacggag cccaatacca aaattggcat tggtaccgtg gatggcatac
     4381 aacctgccaa tataaatcgc gtacagcccc atggggttat cgggccccgc aggaacaaat
     4441 gcgggcaaac tctcccctcg tttcgcatat tcgcgccggg tgttcggcgt tggcgtccag
     4501 gttggtgctt cttgtttacg ttcaacggta gtcacccagt tacgcggggt ttctcgccca
     4561 gcctggccga taccaatagg aaagacttcc actgtattac tgtctggcgg atagtaataa
     4621 agacgcattt cagcgacgtt aaccacaatc cctttacgaa cagtggcggg caaaatcagt
     4681 tgctgcggaa tggtgagttg tgagccaggc ttcggcaaaa aaacatcagc gcccggattc
     4741 gcttccagca tattacttaa cccttgcccg tattgtgcgg caaaagtctc cagcggctga
     4801 gtattgtgat caggaacagt tacagtaagc gactgcccca ctaaacggct accctctgga
     4861 ggtaatggat aagttaccgc caggctattt tggctggcaa aaagcagagc aaatgagcaa
     4921 aaaatattta cacgacgcat catgtccctt tcctaagtcg cgaaagctat ccgttaagta
     4981 tagcttttat cagacttttc gtttttaact gttcaaatca gaagtcgtat tccccggtag
     5041 aacaatatta ctggcagcaa gttcgcccat gttgttatat atcgcacagg cagcttcgat
     5101 gatgggcatc gccagagctg cgccactccc ctcacctaaa cgcatctcca tattgagata
     5161 aggctccagc cccaaatgcg acagcgcaat acgcgcccct ttttctgccg acaggtgaga
     5221 aggaatgaga tacggtttga ttgcaggaga catctggcag gctgcgagcg cggcagcata
     5281 agaaagaaat ccatccagca acacgggtaa accgcaggaa gcagcgccta acatcacgcc
     5341 agccattccg accaaatcaa atccacccac ttttgccagg acatcaacgc catcctgcgg
     5401 atttggttga ttcaacgtaa tcgcccggcg cacaacatca attttattag ccagtttatc
     5461 tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga tcccggccag tgattgtgct
     5521 gactattgcc gctgccggtg tcgtgtttgc catccccagt tcacctacac caaacagcgt
     5581 gacaccgttt tttgccagct cccgcgtata acatatgacg tccaaaagca acttttcagc
     5641 ctgacggcga ctcattgccg gagctgaagc aatattgccg ctacctcgtg cgacacgcat
     5701 gttgataagc ccggggatag gctcagcact atcaatacca acatcaacta cgtggacgtt
     5761 agcgcccgct tgtgctgcca gcacacacac gccggttgtt ccgcgggtca tattttcagc
     5821 ctgaatggct gtcacttctt ttggggaaat agcgacccct tcctcccaga cgccgtgatc
     5881 ggcacacata accagtaccg cttttttgcc cacatgcggt atgccattca accccggcat
     5941 tcccgccagt tgtatggcaa gcgcctccag ccttcccagg ctaccaacag gtttgagtaa
     6001 cccgtcaata tgccgttgtg cacgcgacat agcggcagga tcgatagcag ggatcgtatt
     6061 cagtaaatcg gcaagtgttt gcatgtcacg tctctttata gcagtgccag caggaagacc
     6121 agttcaccaa gctctatagc agctcccagc gtatcgccgg tttgcccgcc cagcgtacgt
     6181 ttgagaagct gaccgaggat gaaaatcgcc accatcgtca ccaccatagc agccacacca
     6241 tgcatacccg gcaataatac ggcagcaaaa atagcggcta agccgagtgt gacgcaggtt
     6301 tgtcgcccat caattttgcc aatgaacaca ttgccaagcc cttcttcccg cgcataacga
     6361 tgacgataca tcaataatgc ggcagtcccg cggctgaccg cacatgctgc cgccagcgat
     6421 gcaaggatcg gttcgccacg cagagccaac tcgcttaata ccagaatctt tgccagtaca
     6481 acaaaaatca atgccagacc gccatgggtg cctaaacggc tatcacgcat gatttccaac
     6541 atgcgatcgc ggctacgtgc agaaaatacg ccatcacagg tatcggcgag gccgtccagg
     6601 tggaatcccc cggtcatcag cgccagcaca agtacgctaa acagtgccgc cagtggtacg
     6661 ccacaccatg cctgcagcac catgaagacc agcccgctaa tcgcgccaag caataatcca
     6721 attaaaggaa aagtaataat accgcgagaa taatgctcga aatccagtcc ctgggaccag
     6781 cgacgcggta caggcagacg cgtaataaat gagagcatcg cccaaaataa tttactcatt
     6841 tgatttttac tccaataccc gaaaccacca gccatacttc atttgccgca gcggccaact
     6901 gctgatttac ccgcccggca atatcacgaa aatgtcgtgc cagacgactc tccggcacaa
     6961 tccccattcc cacttcgtta gtcactaata caacctttgc ggggcaacgt tggcaggcag
     7021 caatcaacga ctgaatctca gcattaatcg cctgttccat cgcctgataa tcccattcat
     7081 cagggtcttt atcgccgcca taatcaaaca acagattagt caccattgtg gtaacgcatt
     7141 ccagcaacac aacctcattc gggttaatgt ctgcatgaat taattcatca agatgttgcc
     7201 agcgctccac tgtgcgccag tgctccgggc ggccttgccg atgatgttct atccgtgcag
     7261 ccatctcatc atcaaggatt tgcgaggtag cgatatacag aacctgtgaa gagtccccaa
     7321 taagcgcctc tgcgtggcga ctcttcccgc tccgtgcgcc ccccgtcacc aaaatcatca
     7381 taccgactcc tgattcaaat gtatgatggt attcattgaa cagaacaatg gatcccccac
     7441 cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc gtaatataat taaaaatcca
     7501 gcattctcaa tccattacga aataagatgg cattacggaa tagccagact ttctcgtgtt
     7561 aacgtccgac aaaatgtgtc attgacagcg gtttctgtgg attgacacat ttcattcaga
     7621 taatgaatta atgctactgc cggaccaacc agcaaacggg cattgtgttc tgaaatccag
     7681 gctattgatt caacgtcagc gacagatctg cgctgtaaaa gaacttgtaa aacaacgcaa
     7741 tagaattatc cggaatggtg gcgactatgc actagaacac cacaatttcg ctctctcggt
     7801 aaggcactgt cacagcattc aacagaatgt gacttgccag atttattagc gccaccagat
     7861 gtttaaccgg gtaaccacga cccagtcggg gatatattcc atcttctcgc tgacatcatc
     7921 aagaataaaa aggtttatca cactcagagc aacgttcttc agcaggcggg ataattcttc
     7981 aacatttaaa agatgtgtta ttaaaggcat acaaactgga cgtattgtat tttcttttgg
     8041 tggttgacct aaggtagcag tttatcctga tgcgctgaga tttctgtaat atctacgtca
     8101 acattctcct cgaatagaaa atgcagcccc ggcaagtgat tcacattttt taacaaaacg
     8161 ttgttagcgt gccattttca gaagctctcc aagaaagcga attcttttct caaattctgc
     8221 attctcagac acagctttct actccatggc aatggcccaa atacgaagtt gctcaggatc
     8281 attagtggtg ctgagaagtt aaataaccat gcggagtacc actcgtcatc acgtatttat
     8341 atatcatgaa gctattgttg tgttttgtac atcagtagat gtattctgat atactccttt
     8401 tgctagacat aacctttcac ctgcttgcaa agcttctgtg ttctgacatt gccaaattgt
     8461 tgcaattctg tatccagcct tctttcagtc atagcttcgg gccgcgataa gactcactga
     8521 tctgaccctg attcctcttg cagactttat agaccaatta aaatgcagtt tctgcaggtc
     8581 aacgtctgac catcattgtc atcactctgg ccattagagt aaccttctgc attcatcctt
     8641 ttgtaaaaag tttatagact ggccccctga atctccagac aaccaatatc acttaaatac
     8701 cccgttcgat ggggcgcgaa ggagccgggg cctgaacgat gcgatcgcac cgacggcagg
     8761 catgcttttc acggactgtc cggataaccc ggaaggcgct gcgcatcagc tccagctgtt
     8821 cggcggcatc ctcacccagg taactcagcg aaccaccgca ctccgggcaa cacggctcag
     8881 tcggcagcag ccgcttctca tcccgtggga gtgactcagg gaacggtttg cgggtgcggg
     8941 tctgacgcag cgggcgctgc actgccgggt catccacccg gccggtcagc gtatcgcttt
     9001 cctgctgcag caggttcagg tcagcttcca tttgcgcgat acggcgggat accttttcgg
     9061 aacgactgcc gaagttcatc cggcgcagct tatccagttg cgcctgcaga tggtctattt
     9121 cgcgttcccg gttgctcagc ttttcctgca gggcgtggat caacgcttcc tgttcggcca
     9181 ggcgctgttt cagcagaagg atgtcgtcag aagaggtgtc gttcataagc ctgtatttta
     9241 ccaggcttat tctgcgacaa ccaggataaa gagcgctaca acatggtcag ggaggtcagt
     9301 aaccgctttg gctgtcgcca gtcgatacct tccatcagca tcgccagttg cgccggcgtc
     9361 aggaacactt tgccatcgcg ggctgagggc caggcgaagc gaccacgttc tagtcgcttt
     9421 gtcagcaggc acagaccatc gccggtggac cagagcagtt ttacctgact gccgctgcgt
     9481 ccccggaaga tgaagacgtg gccggacatc gggtcatctt tcagcgcggt ctgcaccttt
     9541 gcggcgaggc cattgaagcc gttgcgcata tcggtgatac cggcaaccag ccagattttt
     9601 gtgcctgcgg gaagatttat catacaggcc cccgcatcag ttcgcgcagg acagtgctca
     9661 tgagttcagc agagggatga gtcagtgtta tttctcctcc ccgacatttt atgtggcagg
     9721 tagcgggagg agagcaggat ggtagttcga aggttgggag agaagagccc gcctgaagct
     9781 gcacgggtat cagggcagga agcgacgatg agttttttcg gggccgacag acccgccctt
     9841 cacgctgcca gagccttatc catttaaaga gcaggttatc attgacgtca tgtttccggg
     9901 ccagtgcggc aatactgcct tcttcttcaa gagccagcct gaccagttcc attttgaatt
     9961 caggggaata gtgtttccgg ggaagtcttt ctttagcgcg catgaggtgt ccacctgcaa
    10021 aataagtggg tgcttaagtt acaggatcct ggattcggga ccagacggta ctcagacgac
    10081 gcttacagag aatgtccgga atgacgaggt tatctttgcg gagattaagg caatggtata
    10141 cgggccgacg ttccatgccg cgttactaca ttcagcccgg acgtaaaatg cgcccgtcaa
    10201 aaagaacggt tcgcttcgaa actcagccag gataccacct ctccgccaca cccgggaaga
    10261 agttgatatg gtggttgccg gacaacagcg ctcctggatt gactgctgca tcactcaacc
    10321 acgctgaata tcaaaggaga gcgttaccgg ttaaaagaga aacgtaaagc tgtggtgatg
    10381 acccaaaacg caacgcctgt cagtgaggat gaaatggtga aaagcggaca gcatcagtaa
    10441 cggaagtatc ttagcgggta cgaaaatggc aggatcgtga cattgacaga atatctcctg
    10501 atcatcctga atgactgcag ttgctgtaga aaagtaagct gtctccaccc acggcaccac
    10561 ttaacaggca ttccgtaact tcccccagac ttactatgac gcattatcgg tgcactgaaa
    10621 acgggagagt gcgcttaaaa ccgcagaccg catgcgggtg ttactgcatg acagtctggc
    10681 cgtattgtga gatgtcggca ggaaccgatg tccgcgcagg ggggaacttc ctgcagtacc
    10741 tgacgcttca aatggtatta accgtagggt gttgttggtt ccggatcgcg gaaatggtag
    10801 cacaggtctg ctacggggtg tacctctgac agtatatccg gctcgctcat ctgacagcct
    10861 ctctcccgga aaagaccaaa aatgagatcg tctcctcaac agcagagaag aggtcttgac
    10921 agacaggcta tattgcatac gatttatgaa gaaaaagaca gcaaatatgg aggttatagc
    10981 ggagaatacc ctacgaaatt cctatgaaaa acgattgaaa aaaatatcaa attcgattcg
    11041 tttttatatg ctttttggga gcgtggcgat gacgaactgt tgcatgtgca gaggagaatg
    11101 cacccggaag caccagaatc atccagaaag gcagaacgat tacgcat
//
LOCUS       DAZUGK010000147        11147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZUGK010000147 DAZUGK010000000
VERSION     DAZUGK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.148"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(225..944)
                     /gene="dcuR"
                     /locus_tag="ACHY5W_003426"
     CDS             complement(225..944)
                     /gene="dcuR"
                     /locus_tag="ACHY5W_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFS4448262.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(941..2572)
                     /locus_tag="ACHY5W_003427"
     CDS             complement(941..2572)
                     /locus_tag="ACHY5W_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4448263.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTN
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2753..2983
                     /gene="yjdI"
                     /locus_tag="ACHY5W_003428"
     CDS             2753..2983
                     /gene="yjdI"
                     /locus_tag="ACHY5W_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFS4448264.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2995..3267
                     /locus_tag="ACHY5W_003429"
     CDS             2995..3267
                     /locus_tag="ACHY5W_003429"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4448265.1"
                     /translation="MEIREGYNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3494..3790
                     /gene="ghoS"
                     /locus_tag="ACHY5W_003430"
     CDS             3494..3790
                     /gene="ghoS"
                     /locus_tag="ACHY5W_003430"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS4448266.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3818..3991
                     /gene="ghoT"
                     /locus_tag="ACHY5W_003431"
     CDS             3818..3991
                     /gene="ghoT"
                     /locus_tag="ACHY5W_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFS4448267.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4110..5627)
                     /gene="lysS"
                     /locus_tag="ACHY5W_003432"
     CDS             complement(4110..5627)
                     /gene="lysS"
                     /locus_tag="ACHY5W_003432"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4448268.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5864..7321)
                     /gene="dtpC"
                     /locus_tag="ACHY5W_003433"
     CDS             complement(5864..7321)
                     /gene="dtpC"
                     /locus_tag="ACHY5W_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFS4448269.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(7380..9527)
                     /gene="cadA"
                     /locus_tag="ACHY5W_003434"
     CDS             complement(7380..9527)
                     /gene="cadA"
                     /locus_tag="ACHY5W_003434"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFS4448270.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9607..10941)
                     /gene="cadB"
                     /locus_tag="ACHY5W_003435"
     CDS             complement(9607..10941)
                     /gene="cadB"
                     /locus_tag="ACHY5W_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFS4448271.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         3045 a         2614 c         2634 g         2854 t
ORIGIN      
        1 tgggtaaaaa tacacataat gcgggtgaca taatagttaa ttaacttttg ttagcgtttt
       61 gaaattaaaa acaccgttca cctgaagaga tattaatttt tagcgatgat ggagggataa
      121 ttatatttga tctggcacaa gttttactga tgaaggatgt aacttgtgcc agggatattt
      181 tgcattacgg taataattat tacttacaga taaccagcga tccgttattg gcaatattgt
      241 ttcagtagtg agtagtgttc tgcctgaata cggtaacggt aaactggacg ccccgtgacg
      301 ccataatgga tactggtgaa caagatgtgg cagttgacca gccagatgag gtatttacgg
      361 caggaaacac gcgaaatgtt aacctcgttg gctagctcgt cggttgaaaa ctcatagtcc
      421 tgatgcgcgt caatccactg gcacagcgtg cgtaacgtct gcggcgttaa gccttttggc
      481 aagcgacgag gatcctgctc gttggagctg ctgccgtgga ttagctgatc aagctcggcc
      541 tggtcataat actgatgttt ttccagcgcc attttctttt gccgccagcc ggtgagcgcc
      601 tcttcaaagc gggaagcctg gaagggtttg atcaggtaat ccacgacacc gtaatgcagc
      661 gaatctttaa tggttgccgc atcggctgcg gaggagatga caatcacatc acttttgcaa
      721 cgcgcgttat gcaggacagg cagtaaatcg agcccgttct ctttttgcat atagatatcg
      781 agcaatatca ggtcgatagg cgtatcgcta ttgaagataa tctctttggc tttctccagc
      841 gtcgaggctg ttccacagca ttgaaagcct gggatttgtg ctacgtatcg gcgattcagc
      901 tccgcgacca ttgcgtcgtc atcgataatt aatacattga tcatctgttc gacctctccc
      961 cgtcccaggg tatctggaca aaaaattgtg tgaaaatccc gggttccgat tccacggcga
     1021 tgctgccgcc gagattttct acctgttgtt tgacaagtgc taaaccgacg cctcgctcgc
     1081 ttccttttgt cgagacacct ttgtcaaaaa tgtgatcgat tttgtcgggt gcgatccccg
     1141 gtccatcatc gttaacttcg cagtgcagcc agccgtgacg gtagtgcaat gttacgctaa
     1201 tttcgccacc gggttccggc cctaatgctt ccagcgcgtt ttctatcaga tttcccaatg
     1261 tggtaatcag cgtcgcgacc tggtcctcac tgccgctgtc tggcagctgg ctttcactgt
     1321 ttaaaatcag cgtatggcct aaatcggtcg cgcggttaat cttgctgatt aaaaaaccag
     1381 cgataaccgg agatttgatc ttacccagca gagagccaat ctcttcctga tagttattgg
     1441 ctgttttgag aatgtaatct tccaactgct tataactctt cagatgcaat aatccgagaa
     1501 tcacatgcaa tttattcata aattcgtggg atcgttcacg aagtgcgtca gcatagttga
     1561 ccagaccatc gagtcgctgc atcagtttac gtacttcagt tttgtccctg aaggttgaaa
     1621 tggcaccgat gataacgcca ttactgcgca ccggaacggt gttgatcagt aatagccgat
     1681 ctttaatcgt aatctcttcg tcgcggcgcg gggtaccgtc gcgtaacact tccgagacat
     1741 ctaccacctg tgaccacgag tggctgagcg tcgacagttt ctcatcgtcc tgcgacttac
     1801 ggtaattcag caattcttgt gcggcatcgt tgatcagcgt gacctcgccg cgatcgtcca
     1861 cggcaacgac gccttctttg atagactgca acatggcctg gcgttgctca aacagcgtgg
     1921 agatttcgta gggttccagg ccgaaaagga tttttttcag tactttaacc agaatgcagg
     1981 tgccaatcag tccgaccagc atgccaaata atactgacca gataatgctc cagcgactgt
     2041 cattgatctg ttgggtcacg cggcttaact caaggccgat cgccaccacg ccaatttgtt
     2101 tatgattttc atcgtagatg ggggtaaata cgcgtaaagc ctgcgccaga aaaccgcgat
     2161 tgatagcgac attttcttcg ccattcagcg ctttaaggat gtcatcacct ttaaatggct
     2221 gaccaatacg ctgggcttca ggatgcgagt agcgaagact ttgcatattg gtaacgacaa
     2281 taaacagcag atcgttgcgt ttgcgtacgg cttctgcgat ggcctggatg ccgctctcct
     2341 gcggtttttt ctgcaagccc tgacggattt ccggcgagtc ggcgagagta cgcgccactg
     2401 ccagtgcctt gttggctagc ccgtctcgcg tcatatcact gatttgcgag aagtaaatca
     2461 gatgcaccac caatagcacc gagaacagta ccgcgctgac cattaagatc actgtggtac
     2521 tcaatttcat cggacgtttg cgtaacatgc ggtagggcaa tgaatgtctc atcagcttcc
     2581 ttgtgtgaca aatttcttaa gcattatctc tgatgaggcg ggtaattcaa agggagtaag
     2641 aatgattggc tatatagggg aagagactct ggcaacggaa actgccagtg ctgtatgaag
     2701 attccggggc tatgcttata gtgaaaacca tactgatgag agagggaagg tcatggatca
     2761 ggcgctactg gacgggggtt atcgctgtta taccggcgaa aagatcgatg tctatttcaa
     2821 cactgcgata tgtcagcatt ctggcaattg cgtacgtggc aacggcaagt tatttaatct
     2881 caaacgaaag ccgtggatca tgccggatga agtcgacgtt gccactgtgg tgaaagtgat
     2941 tgatacgtgc ccgagtggtg cgctgaaata ccgtcataaa taagcgaggg taaaatggaa
     3001 atacgcgaag gctacaataa attttacatt aatgacgaac aaggcaagca aatcgctgaa
     3061 attgtctttg tgccgaccgg agagaattta gcgattatcg aacataccga tgtcgatgaa
     3121 agcctgaaag ggcaagggat cggtaaacag ctggttgcga aagtcgtgga aaaaatgcgt
     3181 cgggaaaaac gaaaaattat cccattatgt ccatttgcga aacacgaatt tgataaaacg
     3241 cgggagtatg atgatattcg cagttgatgg aagagtacag attcacgata tttttcatgc
     3301 tctccgcgga gtgatgcagg agagcatctg aagggtaggg ggatgcacaa agaatgggca
     3361 gagagagcgt ttttttgtcc caagtcatcc cctttactga gcaaaaaaaa gaatatctcc
     3421 tatatgagaa tcatcaatcg gggttaataa gttttgcgtc cccagagcgt ttaatattga
     3481 taggagtcat attatggaag gtaaaaacaa gttcaatact tatgttgttt cttttgatta
     3541 tccatcatct tattcctcag tgttcttaag gttaagatca ttgatgtatg atatgaattt
     3601 ctcttctatc gtggctgatg aatatgggat accacgacaa ttgaatgaaa attccttcgc
     3661 aataacgaca tcgttagccg caagtgaaat cgaagattta atcaggctta aatgcttaga
     3721 cttaccggat attgactttg acctcaacat tatgacagtt gatgactatt tccgtcagtt
     3781 ttacaaatag ccaggaagga taagaagaac ataaaaaatg gcactattct ctaaaatatt
     3841 aattttttat gtgattggtg tgaacatatc ctttgtcatt atctggttta tctcacatga
     3901 gaaaacacat attcgtttac ttagtgcatt cctggtcgga ataacctggc caatgagtct
     3961 gcctgtggca ttactttttt ctctctttta ggagctgtag ttgagtggac ccgctggtta
     4021 tcgtgaaacg aagcgtctct cctcaaaact ggatttgcgg aacgatgaac gaagcagtcg
     4081 cctgactgct tcgttcatta aagtgaaatt tatttctgtg ggcgcatcgc cgggaagaga
     4141 ataacgtcgc gaatagtatg gctgttagta aacagcataa tcattcggtc gataccaata
     4201 cccagaccag cggttggcgg cagaccatat tccagcgcag tcacgtaatc ttcgtcatag
     4261 aacatggctt cgtcgtcacc tgcagcttta gcgttaacct gttcctggaa acgttcagcc
     4321 tgatcttctg cgtcgtttaa ttcgctaaaa ccattaccga tttcacgacc accgatgaag
     4381 aattcaaaac ggtcggtgat ttccgggtta acatcattac ggcgcgccag cggggacact
     4441 tctgccggat attccgtaat aaaggttggc tgaatcagat gtgcttctgc cacttcatca
     4501 aagatctccg tgacaatacg tcccagcccc cagcttttct ctaccgtaat tccgatagat
     4561 tcagctaatg ctttagcagc atcaaaatta tccaggtcgg ccatatcggt ttctggacga
     4621 tattttttga ttgcttcgcg catggtgagt ttttcaaacg gtttgccgaa atcaaacaca
     4681 tgctcgccat aagtgacttt agtggtaccc agaacctctt gtgccagggt gcggaacagt
     4741 gactctgtca gttcaatcaa atcgtggtaa tccgcatacg ccatgtagag ttccatcatt
     4801 gtgaactcag gattatggcg aacagaaata ccttcattac ggaagttacg gttgatttcg
     4861 aatacccgtt caaaaccgcc tacaaccaga cgtttcagat acagctccgg cgcgatacgc
     4921 agatacatat ctaaatccag agcattatga tgggtaataa acgggcgagc agatgcccca
     4981 cctggaatta cctgcatcat cggggtttct acttccataa agccgcgcgc gaccatgaac
     5041 tgacggatag cggccagaat ttttgaacgg acaacaaacg tttgacggga tttatcgtta
     5101 gcgatgaggt ccagataacg ttgacgataa cggacttcct gatcctgcag accatggaat
     5161 ttatctggta aaggacgtag tgcttttgtc agcaggcgca actcagtaca gtgaatggaa
     5221 agctcgcccg tttgcgtctt aaacagcgta ccgcgggcac cgataatgtc acccagatcc
     5281 cattttttaa actgatcgtt ataaacacct tctggcaggc tatctcgtgc gacgtacagt
     5341 tgaatacggc caccgacatc ctgcaacgtt acaaaggagg ctttccccat gatacgacgg
     5401 gtcatcattc ggccagcaac cgagacttca atgtttaagg attccagttc ctggttatcc
     5461 ttcgcatcaa actcttcgtg caactggtca gaggtatggt cgcggcgaaa atcattggga
     5521 aacgccacac cttgctgacg tagtgccgcc agtttttcgc ggcgatttct cagttcatcg
     5581 ttaaaatcaa tagcctcatt ggctccccgt gtttcttgtt cagacatttt ggttcctcta
     5641 aatccagctt tcaatttagc gtagataaag agacagatcg gtacactgta ttatctgcct
     5701 cgactattag taactcagtt tatcttatcg aaactaacgc ttacctcaag ttgatatcac
     5761 taataaataa ccatcaaaat ggtttaaatg acttattaat atcgatttgc tagtgttata
     5821 aactgaaagt taatttattc ataaccaatt gtttttacga cagttaatcg ttgctctcct
     5881 gtaccatatt cgtcggcgtg ctaaacagga aacgggtagc aaaggccaat acgacgatga
     5941 tcgcgacaca agccaacgtc cactctccca tctgagaaaa gaatcgctgg taagctgcaa
     6001 ttgccatacc gctaatttgc gactctgtcg tctgctgtgc cactacgcct gccagccagt
     6061 tggcgaccgc gcctgttgcc agcatataaa tcccggttaa tacgccagac attttcagac
     6121 gcgtgatttg cgcaatcgcc accgggtcaa taaagagttc ggcaaagccc attagcgcca
     6181 acccggatat catcacgccc attgacgctt gaccgtcagc cgctgcatgt cgggcatcaa
     6241 atgccagcaa cataaagcca caagccatca gcagtaagcc aaaggcaaac ttcagccaga
     6301 cgcgcaatgt tgagttgccg tggctttctg gcgacgccag ccaggccaga acaaccccag
     6361 cgagcatcac cgcaattgca ttcaccgact ggaatagtgc agtaggtact tcaatattga
     6421 atgcctgacg attcacaaag cgatcgataa acagactgat ggtactgccg ccctgttgtg
     6481 ccagtaccca gaacaatgtc ccgacaaaca tcaacaatac aatttgccaa agagcacggc
     6541 ggtgttcggg gaatttgatc atcatgcggg caatgatttg tgcggcgata aggcaaacga
     6601 tcgccagcaa atatccggac cagtcgttct ccagcagcag agtaaaaaat actggggcta
     6661 aacagagcat caccactaac cagctccata ctggtaaggc aaatttgaca ctagtgagcg
     6721 cttttttatc catactacgc gtggactgga aatgacgatg cccgcttaag aaaatcaaca
     6781 aaccgataaa catgccgcca cccgcaaggg caaagccaac atgccatcca taccactgag
     6841 cagccaggcc gcaggcgatt ggggcggcga tagaaccgat attgcccgca gcatacagca
     6901 gcgaaaaacc gccatcacgt cgatgatcgt tctcgtcgta gagctcgcca agcaaacagc
     6961 tgatgtttga tttgaataaa ccgtagccac aaataatgat tgccagcgcc agatacaggc
     7021 taaaggttga atttgtatca atgcccagca ccacatggcc aagagtcatt aacagcgcgc
     7081 cggcaatcac tgcagtgcgg ttgccgagca ggcggtcggc aagccagccg ccgagaatag
     7141 gggtaacgta aaccagagaa gcatatgcgc tgaacaggct gatggcatgg ttatcatcaa
     7201 aaccaagctg atgggtgaga tagagaatga gtaaggcacg catgccgtaa aaactgaagt
     7261 actcccagat ttggatcgcc acgatatagt atatcgcgcg cggctgtgag ggtgttttca
     7321 tgtgttctcc ttatgagcaa aaaagggaag tggcaagcca cttcccttgt acgagctaat
     7381 tattttttgc tttcttcttt caatacctta acggtatagc ggccatcagc ctgacggtat
     7441 gcaccgtgaa tatcggtttc aaagcccgga tagtgagcgc cgatttcaca cagcatctgc
     7501 aggaactcca gaaccggacg gctttcttcg gtgatcattt cacccggcat taccagagga
     7561 actcccggcg gatacggaag gatcatattg gcgttaatac gacctaccat ttcgtcgagg
     7621 taaacttctt cggtcatacc gtgcagctct ttctggaacg cagcatacgg agtcattacc
     7681 atcgtcggca gcacttcaaa tgcgcgatac atcagatccg gcagattgtg gtgaacaatc
     7741 agtttgtgga tattctgagc cagttcctga atacgcatgt tttcatagaa ttcaggatct
     7801 tcacgataca gagacggcag catgtttttc acacgcaggt tcaggtcgaa cgcacgtttg
     7861 aagtcagtca gagcacgcag caggctcagt gctttggtct tatcgatacc gatgctgaac
     7921 aggaacagca ggttatacgg accggttttc tcaacaacga tgccatgttc gtcgaggtat
     7981 ttcgccacga tgctggccgg aataccgaag tcgctcatgg tgccgtcttt ttccatcccc
     8041 ggagtcagca gggtgacttt gatcgggtca agatacatgt gctcattatc gatgtttttg
     8101 aagccgtgcc aggtgctgtc agaacgcagc ggccagcatt cagtcgtatc gatatgatcc
     8161 ggctgccata catcaaagaa ccagccatca gattccgttc tcagacgttt gatctcttta
     8221 cggaatttga tcgcacgttc aatggaaccg ttgatcagac gcttaccagc attacctttc
     8281 atcatcgccg cagcggtttc agtggacgcc acgataccgt agtgcggaga agtggtggtg
     8341 tgcatcatgt aggcttcgtt aaaggtttct tcgtttacgt cacctttaac gtggatcatg
     8401 gaagcctgag agaacgccgc cagcagtttg tgagtagact gggtttcgta aatcactttc
     8461 ccttctacac ggccaccgct cataccgcat ttaccttcgt aaatcggtga gaagttggtg
     8521 taaggcaccc acgcggagtc aaagtggatg gatttcacat ccagtgtttt cttgatgaag
     8581 tcggtgttgt acagcagacc atcataggta gagttggtaa ttacagcatg taccggccag
     8641 gttgcgtttg gtgtttcttt cacgcgctta gcaatggtag cgtgctggaa ttcactctgt
     8701 gggataccac caagaatacc gtaagcgtta cgggtcgggc ggaaatagat tggcgtaaca
     8761 tcgctcatca tcatcaggtg ggtcagcgat ttgtggcagt tacggtcaat cagaatggtg
     8821 ctgcctgccg gagcagagta cataccaaca attttgttcg cagtggaagt accgttggtc
     8881 accatgtagc tgcggtctgc gttaaagacg cgagcgatat actgttctgc ttctttgtgt
     8941 ggaccactgt gatccagcag agaacccagt tcagatactg aaatggaaat atcagatttc
     9001 atggtattcg gaccaaagaa atcatagaac aggctaccta ccgggctttt ctggaatgca
     9061 gtaccgccca tgtgaccagg agtacagaaa gtatatttac cttcacgaac atatttaaac
     9121 agtgctttag tcagcggagg cagaatagtg ttgatatatt cgtcagtggt ctgcttgatc
     9181 ttgttagcaa tatcttcagc agcacccagc gcatattcaa agaagctaat ctgtaaacgc
     9241 aggtcattca ggcttacatc gagagtggaa tacgtattag cgaacgcgta caacggcagg
     9301 ttctcgttca ttttgctaat ttcttcgcac agctcgagat tatatttatc ccagtcaaaa
     9361 ataacgccgc acagacgcgc attgttttcg atcagtttta ataagtcgtc acggtcgttc
     9421 gggtaaacaa tctggaagtt cagacgttca agcgcgcgat gaagttcacg gatgggttct
     9481 tctttaaaat aaacccccat gtgattcaat attgcaataa cgttcatagt catatctcca
     9541 ggtaaaaaag gcccctccca acacatggga caaaatgaaa ggaggagcct cggaaaatac
     9601 ttttaattaa tgtgcgttag acgcggtgtg gttatccatt gagtggctct ggcgctcgtg
     9661 cattttgcga gcgtagaaca tcaggataat caggctgacg atgaaggtac ctgccagctc
     9721 gaaggagctt gcgcccatca gcgcgatgaa gcagaacacg caacccagta cagagcagat
     9781 caggctgaca aagttgcgga tgttaacgcc ttcaaaacga atcaggtcaa cgcaagagta
     9841 gaagtacggc agcatagtca gcagtactgc gataccggtc agttcaccga acaggtcaga
     9901 tgctttacca ccggcagagt tcatcagagt gatgaggatc atcagggcag tcattttcac
     9961 tgcagccagc agcagacctt ttttcggaat accgttgctg tcgacttcac cataaacttt
    10021 cgggaagtta ccgtcgttag cggcacgtac acctgcctgg cctaccaaca tcatccagga
    10081 gcccagagaa gtcaggcacg caaaggcggt gaatgcagaa accagcggcg cagcccagtt
    10141 accgaggata gttgaagcac tgattgcaaa cggagcaccg gaagccgcca ttacagaaga
    10201 cggatacata ccggaaagca cctgagtcgc agcgatataa acaatacctg ctaaaccagt
    10261 acccagcatg gttgccagcg gaacggtacg tttcgggttt ttaaccatac cagtacttac
    10321 tgctgcggat tcaacaccca cgaaggccca caggcagagc agaatacttt taatgatcgc
    10381 atgaccatca gtggtatccg cagtattcca gttagctgca taagttgccg catcaaacca
    10441 atgccagcca acaatagcag tcatcaccac aggaataaga accagcacca gaccaatagt
    10501 ggttaaacgg cttacccagg taccgccgag catatttaca aaggtaaata cccagacgat
    10561 agcaatacag gcgatacccg ccggaacagg atcatttaat actgggaaga aggtggaaag
    10621 ataagataca gcggtaatac caatcgccag gttaccaatc cagttagcat ggtaataaag
    10681 aacacctgtc tgaaaaccaa atgcaggaga aatttctccg gcataagcaa ttgggccacc
    10741 ttgttgcggg ttttttgttg ccagtcgggc atatacatac gccagcgaca ttgcaccaat
    10801 aatagagata atccaacccc agatagcaat accaccgata cttgctaggt tcgcaggtaa
    10861 taatgcaata ccgctcccca tcatattacc ggcaacaaca ccggtacagg caaatagccc
    10921 gatcttcttg gcagaactca tgctcttctc ctaatttcat ttttgaattt ggagtccggg
    10981 tcatgatgta taactatttc ctgaccagac caaactggcg ataagattac tcacgaaaaa
    11041 aggattaatc ctaaagatta ggtgaaataa cacaaaagtt tctgtaagtg agaacttgag
    11101 gttttttatt aacacatcag gatcgcaagt tgatatcatg aaaagat
//
LOCUS       DAZUGK010000148        11098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZUGK010000148 DAZUGK010000000
VERSION     DAZUGK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.149"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..277
                     /locus_tag="ACHY5W_003436"
     CDS             <1..277
                     /locus_tag="ACHY5W_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448272.1"
                     /translation="TYSLADCKRLRNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKD
                     NNSQKKALLIISLIYFISGLVIFLSYTKFLYANKKVVEYKASNKNHS"
     gene            complement(349..801)
                     /locus_tag="ACHY5W_003437"
     CDS             complement(349..801)
                     /locus_tag="ACHY5W_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS4448273.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKNGKKIDYPARPLLGFTGEDVQMIEEIILAHLER"
     gene            complement(794..1261)
                     /locus_tag="ACHY5W_003438"
     CDS             complement(794..1261)
                     /locus_tag="ACHY5W_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4448274.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPATRPMELYINGELVSKWDE"
     gene            complement(1224..1469)
                     /gene="lysC"
                     /locus_tag="ACHY5W_003439"
     CDS             complement(1224..1469)
                     /gene="lysC"
                     /locus_tag="ACHY5W_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796289.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFS4448275.1"
                     /translation="MLCAGCTSVRPAPTPVIVPNACPKVSLCPMPGSDPETNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(1351..1794)
                     /gene="lysB"
                     /locus_tag="ACHY5W_003440"
     CDS             complement(1351..1794)
                     /gene="lysB"
                     /locus_tag="ACHY5W_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040695.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFS4448276.1"
                     /translation="MSKLMIVLVVLLSLAVVGLFLAKHENASLRTSLDRASNVASGQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCPQRLPQSEPLPDAGQRPGDER"
     gene            complement(1782..2207)
                     /locus_tag="ACHY5W_003441"
     CDS             complement(1782..2207)
                     /locus_tag="ACHY5W_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFS4448277.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFGGREPCQS"
     gene            complement(2222..2726)
                     /locus_tag="ACHY5W_003442"
                     /pseudo
     CDS             complement(2222..2726)
                     /locus_tag="ACHY5W_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
     gene            complement(2726..3007)
                     /locus_tag="ACHY5W_003443"
     CDS             complement(2726..3007)
                     /locus_tag="ACHY5W_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4448278.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ"
     gene            complement(3011..3214)
                     /locus_tag="ACHY5W_003444"
     CDS             complement(3011..3214)
                     /locus_tag="ACHY5W_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFS4448279.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(3214..3723)
                     /locus_tag="ACHY5W_003445"
     CDS             complement(3214..3723)
                     /locus_tag="ACHY5W_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFS4448280.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(3823..4566)
                     /locus_tag="ACHY5W_003446"
     CDS             complement(3823..4566)
                     /locus_tag="ACHY5W_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFS4448281.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYAMKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSATPAKRGRPKKKAS"
     gene            complement(4570..5643)
                     /locus_tag="ACHY5W_003447"
     CDS             complement(4570..5643)
                     /locus_tag="ACHY5W_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFS4448282.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYVSLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(5702..6556)
                     /locus_tag="ACHY5W_003448"
     CDS             complement(5702..6556)
                     /locus_tag="ACHY5W_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFS4448283.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTNRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            6730..8502
                     /locus_tag="ACHY5W_003449"
     CDS             6730..8502
                     /locus_tag="ACHY5W_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFS4448284.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELYNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYEPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            8502..9536
                     /locus_tag="ACHY5W_003450"
     CDS             8502..9536
                     /locus_tag="ACHY5W_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4448285.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWFSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            9912..11030
                     /locus_tag="ACHY5W_003451"
     CDS             9912..11030
                     /locus_tag="ACHY5W_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFS4448286.1"
                     /translation="MTESLDYIDLNKLELDTKNPRLPEGVERTPEAMLNHIALTTSIE
                     DLMNAIAENGFFPGEPLIAVKEGDKYTVVEGNRRLTAVKLIHNPYECDRPSSRMIEIA
                     ESAKDKLGTLEKLPVIVRDTRAEILPYLGFRHITGVKQWEPLSKARYIEQLFGLTSPN
                     SPTNDRYHQVARAIGSRKDHIKRNLDALAVYKVMESNNFYDIDGLDEESIKFSILSTA
                     LADEKIGLFVGVSEKDEYGDITSNDVIIHPHHINRENTRELTLWLYKKDDSGKTKVGE
                     SRNLRLLSSVIDNPKALTSFRNGADLKVAYQLTEDLKQDFMTLLYKAESALIEAAGIV
                     ATIDYNPEALEVARRLSQNVKLIGNTIKAKKVSDDEDF"
BASE COUNT         2558 a         3106 c         2873 g         2561 t
ORIGIN      
        1 aacttatagc cttgccgatt gcaagaggct acgaaacgta tttataaaag acacacttcc
       61 tgagatcgcc tgcaataagc tcttacagct aaacgaggag gactccgaat ggttaagtca
      121 ggagattaaa gataataaca gccaaaaaaa agcattatta ataatatccc tcatctattt
      181 catttcaggt ctggttatat tcctgtcata tacaaaattc ctttacgcca ataagaaggt
      241 tgtagaatac aaagcatcaa ataaaaatca ctcataaacc tctaaacatt gagcgaccag
      301 catggccgct caatgtttaa ttgcgcatca gcctctgcct ggataaaact aacgctcaag
      361 gtgagccagg ataatctctt caatcatctg cacatcctca ccggtaaagc cgagcagagg
      421 acgcgccgga taatcaattt tcttaccgtt tttccgggtt tcttccgaca gaccgaactg
      481 atgcacactg gcgattttcg gcgacttccc gccgtaaaat tccattgatg cctgttccgg
      541 gctggcgcgg atatgcaaaa aacgactggt gataagtttc gcaaacattt ttcgcttaac
      601 acgaccggtc ttttttctgg cgctctgctg ctggcgtggc gcgtagggtg tgccgtccgg
      661 ggctttctgt gccatcaccc gacgctgctg actctgacgc agacgtttcg ccagttcggc
      721 gctcagtcgc cgacgccctg acggtgacag cgattcaatc agtccggtca gccggtcttc
      781 aaaacgctta aactcattca tcccacttgc tcaccagttc gccattgata taaagctcca
      841 tcgggcgggt ggccggctcc ggcggcgtgg gttccgggat attcttcaca tgcagcgcgc
      901 cgtccacctc actgaccagc gtgcgctcgg tcagcatcag gctgatgctg atatcaaagc
      961 tgctgtcatt gttgatgtct gcataaaacg tgaagccctt tttctgacct tcgtcagtgg
     1021 tcatgatgtc gggctgattt tcccgcagcc acgccagcac cggcacgatg agcaggtcaa
     1081 aatcaccggt aaagtcggtc acaatgacat tgagcgtgta acgcttttcg aatgacagcg
     1141 acgtcgccag tgtggaggca atactcccgt tatccacgaa tatccgcagc atatcggggt
     1201 tagttttcag caccgtgacg gcatcagtca gcgccctgcg caggctgtcg ggtttgagca
     1261 tcgttttcgt cctgacagtg tttaatcatt tttacctggc tggcacagcg cgccagcgcg
     1321 ttctcaagct gccggatatc ggcacttaaa tcaccgttcg tctccgggtc gctgcccggc
     1381 atcgggcaaa ggctcacttt ggggcaggcg ttggggacaa tcactggcgt cggtgcaggc
     1441 cggacgctgg tgcaaccggc gcacagcatc aggcaggtca gcaccgtacc agcggcggaa
     1501 atcttcgttt tcattgagta atctcgtgat ggttttctcg cgctgagctt cacgcttcgc
     1561 ggcgttctcc agttcctgac gcagtgccac ctgcgccagc tcgtttttgt ctgccctggt
     1621 gagcgcaaca tgaagctgat ttttcagcat ggtgatggtc gtctgctgcc cgctggcgac
     1681 gttgctcgcc ctgtccagtg aggtgcgcag gctggcgttt tcatgcttcg ccagaaacag
     1741 acctaccacc gccagtgata acaacacaac cagcacaatc atcagctttg acatggttcc
     1801 cgccccccaa aacgctgacg gcaggccgta cgtatcagcc ggaaaaacag tgacgccacg
     1861 agataaatca gcgcggtaaa aatccatccg gcggcgacca gcgagataaa cgtcgccacc
     1921 atcaccacca gagccgctgc ccgtctgcgc cacggcaccg gctgcaaaaa cagcgacgtg
     1981 acaatcttca cggccagcga ttccggcgga agctcccgcc cgtagcgttc cagtacatac
     2041 tctgtggcat acacgccgac accaccggca accacacaga taaccgtcgc cagaatcgcc
     2101 caggcagcga caaaactgac ggccacgctc tgcgggtaaa tcagggacag tgccagcatc
     2161 agcgccagcg acacgttcag catcagtgaa agggataatt tcttcatggt gtttactccg
     2221 tttaagccgg tacgccgcca gcggtacgcc agacggtgac cagtttttcc agtgaatgct
     2281 cacgctgacc gtaaccggca cccggcaccc ggcagggacg cccagatatt gcgacagcgt
     2341 gaaatggcgc gctcaatgcg tcccgcccgg atgtcatcca gcgcaccgcg ttcgcggatc
     2401 aactgaatgg cgagtctgtc ctgtgacaac ggactgaaat ccggcagggc aagctgtttg
     2461 cggtaatgcg gccagaacag gtaaagctgc tgatagcgac cggaggccgt ggatttttca
     2521 ccgcgacggt taaacacctt cgccggtcgg ccatgtgcga acgggtggtc actgtagtcg
     2581 gtgaaaattt ccggctttcc gtccagtccg gtgactatca cgtcatagcc ccggtttttc
     2641 gtcagcggat gattcgccgt cccttcggac acggccagca tgtcgagaaa ggcggcgata
     2701 ttctggtgcg tgttaattac cggcattact gtttccccct gcccttaaag cggcgctgaa
     2761 tggcaatctc aatcacctga taaccggcga tacccagcat ggagccgatg ccgcacaccg
     2821 caggcagtga caggtcagga aactgcacca gaacaacacc ggcaaccatc gagacaaaac
     2881 caccgagcaa catgcgcccg ataaacagac gcggggtgat gggttcacca ccggcaagca
     2941 ccttgccgac aacaatcagc accccaatca tgaaaagcga caggacgctt ttttcttctg
     3001 ctgtcatgcg ttactcccac agattgacag tttcagccac gggcgcggtc tgaacgtcgg
     3061 gcagttcgac ggcggtgccg tgcggcagca ccgcacccag ttcagccagt cccggatttg
     3121 cggcgagcac agtctcgacc acgccctcag tgcgcccgta ataccggaca caaatggcgt
     3181 cgagcgtgtc gccctgtagc gcaaaggtct tcatcagatt tgactcacga tgcagcgcgg
     3241 cttgtcctgg atacgcgcca ccgcccagcg catatcccgc cacagctcat caatggtgct
     3301 gtcaatgctg tcggccttct tgtcgccttt cgcactggca tccacgccgc gataacgttc
     3361 ataaagcgac gcggtcgcca tcgcacacac ggcgcgctcg tagtaaaaaa ctttgatgct
     3421 ttcaccgtcg atgtcgtccg ccgggacgtc cgccagacgc gtaaaaccgg cggcaatttt
     3481 ctgttcgcgg tactcgtaca gctccgcatt cgtctccgcc atgcctgact tgatggcctc
     3541 acgcagacgg gcgggggcga cggtctgctc aaggcgcata cgttcccgga cgcgcttcgg
     3601 gtcgatatcg ggaaaaaaga acgtgttttt aatcaccggc tcgtcgcctg ccggttgcgg
     3661 gatgaccacc gtaccctcac cggacacggg agcctccttt cgcggaataa tcagcgtcat
     3721 catgactacc tctgaaaagt cgggcggtgg acgccggtgc agtgtcaggt gattcaccct
     3781 cactgaccgg cgtgccgccc tggcgcgggg cgcattcggt tgttaactgg ctttcttttt
     3841 cgggcgtcca cgttttgccg gtgtcgcact ccgggtctta cgcggggcgc gggtggccgc
     3901 ttttggctgt ggctccggct tcggtttcag ttcccgctcc agtcgttcaa tctctttttt
     3961 gacgcctgcc tgacagtcga gctgtgtcgc acgttgcagg tgcgccagcg cacctgcggc
     4021 atcaccagcg tcacgcagaa acagaccggt gattttgtgc agctttgcgc gcacttcatc
     4081 aggcatgtca gccgtggcgg taagttcaag ggtgtccgtc agcaggcggg gatccacaga
     4141 ctcaccagca gcgtgggcgc gcatggccgc gagcgccacc tcctcggtga acatgtacgg
     4201 cggggtgcgg cggtgtttac ccggcatggt cagaccgtac ttcatggcat aacgggcaat
     4261 ctccagcgca ccggcaatat cgccggtatc cagacgccac agcatgactg tcatcagaat
     4321 gtcatcctgt gcgcctttgc cctgctccag cacgccgttc acccacggca accagaacgg
     4381 cagcagttcg cgtttttttg cggccttcag ctcttttgaa taaatcgctt tcagtgtgcg
     4441 ctggtctgcg gccagcttga ccagcatctg ctcatagaca gttgcatgtc gcagcggggc
     4501 ggcttcccgc tgcgcggtca tcgctgccga gacccgcatc atgtggcgct gtgcgggact
     4561 cgtcatcggt tacgctcccg gctctgcggt cgctttagcc ggtgtggaga agtcaccgac
     4621 cttaattttt tccaccagac aaccggcggc gtagtcttcc accacgtaat caatgttcat
     4681 tgactcgtag ttctccacgc ggtcgagttt cgggttttcc tcaatcacgc gccgatggct
     4741 gtcatccatg tagtagatgg acaggttttc cagcttcgtg atgagcatcg catccgccgg
     4801 gaagtacggg acgcgtaccg ctggcaggtt accgatgcgt ttctggctga tgatgacgtc
     4861 agcggccagc atttcgctgt tgtcttgctc tttgttaacg atggggaaat acttgtccgc
     4921 cagtagctga cgccccacaa tcaccacaag gtcagggtct tcctgatacc acggttcaat
     4981 caggttgttg gtcgcatcca tcaccagtgc atcgaggctt acataatcac cgcccttacc
     5041 cacgcggatg acctcagagg tggtgcggcc ttcctcgtca gtgaccttgc tcatcacgcg
     5101 cgccggggct tcattgcggt atttctgcag ccagccgacc gccacatcct gcagcatcgg
     5161 attgctgctg cggtcagagg tttcggcacg cttcacgccg ttaaaaccgg ccatgatgaa
     5221 atcaagggac tggcgtttga taatggcgtt acggatacgg agctggaaat cctgataacg
     5281 cgcccacagg tccagcgttt tgtagcggat ataaaaatcg aagttaatct ggtcgcattc
     5341 gtacttgttt gacgccagct tcgagaagtc cttcggctga cgctcggtgc caccggcggt
     5401 gtcggtggtg ctggcgatgg agccggtgac accaatacca attttttccc ctttcatttc
     5461 gctgaccggc acaatgttga tgcgggtcag aaagtcagag gactcctgca tggtgttcat
     5521 cagggtctgg gtgaccgacg gttcaacggt gaattttttc gacacatcac cggcgtcgat
     5581 gccgttcagt tcggcaacac gggacaggta ggcattaaat ttaaagcggg tttcctggcg
     5641 catagttttt cctgaaatta agggttaatc gtgaaggttt tcccggactg actgacgccg
     5701 gtcagcagtt cgtcatcagg gcgtcaccgc caccgccggt ggctttgctg cggcgctgct
     5761 gggtcagact ttcggtgtgg tcgagactgt ttttcaggcg ggtgaatgcc tgactggttt
     5821 catccgccct gttagtcacc tcctgcttaa gtgcggaaaa ggcagtttcc atctcagcga
     5881 ggcgctgctc agtggcgctc agtttttcct gcacatgctc agcgacagcg gtcaccgctt
     5941 catgcacgtc attcagacgg gcgtcatcgc tggcctgttt gcggccaaaa atggatttca
     6001 ccttttcggt cagggcggtg aacacggttt caggcaggtc ttcaaattcc agctcaacgg
     6061 gcgttgccac tgaaatcagg ttttcagggc ttaatttgaa gcggttcaga gggttgtgtt
     6121 ttgccgtgcg gcagaattcc aggtattccg tgccgaggct tgccgggtca tcggtgacgg
     6181 ccagacccac cagataacat ttgcctgtat tggcaaagtt cggctgaatt tccattgagg
     6241 tatagacctt ctgcgcggcc ttgttcatcg cgataaggtc atcggtcggg gtgattttcg
     6301 caaacagcgc ccatttgcct ttcagcgccg aatcatcgtc aatcttttcg gccttcagtt
     6361 cggccacatc gccataacgt ttaaaaatac cgtcaggcag gatgccgcgc agatgttcca
     6421 ggttaatgcg gcaaccatag acacgcgggt caaaggtttc ggccatttcc tgaatatcct
     6481 gcgcactgat gacacgcccg tcacaggtgt caccctcaac gccgatacga aagaattttg
     6541 agactttttt tgccattgtc aggagtcctg aatagtgatt agaggagtca catgtcggca
     6601 tcagtttccc gacgatgcgc atcctccgcc atcagtcccg gatggcttat cactgacaca
     6661 acagcacctt agcgaatcgc ggggcgcgac tcagtagcct tgccgtgtat tcatcacggc
     6721 gaggtattca tgaccatcac cacagacacc actcttttac acgacccgcg tcgtcaggcg
     6781 gcgctgctgt actggcaggg attttccgtg ccgcagattg ccgccatgtt gcagatgaaa
     6841 cgcccgacgg tgcagagctg gaaacagcgc gacggctggg atagcgttgc ccccatcagc
     6901 cgtgtcgaaa tgagtctgga agcgcggctg acccagctca tcatcaaacc gcagaaaacc
     6961 ggcggtgact tcaaggaaat tgacctgctc ggacgccaga ttgaacgact ggcacgggtc
     7021 aaccgttaca gtcagaccgg caacgaggca gaccttaatc cgaacgtcgc taaccgcaac
     7081 aaaggcgggc gtcgcaaacc gaaaaagaat tttttcagtg acgaggccat cgaaaagctg
     7141 gagcagattt tctttgagca gtctttcgaa tatcagttgc actggtatcg cgccgggctt
     7201 gagcaccgca tccgcgatat cctgaaatcc cgccagattg gcgcgacatt ttatttttcc
     7261 cgcgaggcgc tgctgcgcgc cctgaaaacc ggtcataacc agatttttct gtcggccagt
     7321 aaaacgcagg cgtatgtgtt ccgcgaatac atcatcgcct ttgcccgtct ggttgacgtt
     7381 gacctgaccg gtgacccgat tgtcctgggc aataacggcg caaaactgat ttttctcggc
     7441 accaactcca acaccgcgca gagccataac ggcgacctgt acgtcgacga gattttctgg
     7501 atcccgaatt ttcaggtact gcgtaaggtg gcatcaggta tggcctcaca gagtcacctg
     7561 cgttcgacct atttctccac cccgtccacg ctggcgcacg acgcctaccc gttctggtcg
     7621 ggtgaactgt ataaccgggg acgcgccagc gccgccgaac gcgtggaaat cgacgtcagt
     7681 cataacgccc ttgccggtgg gcttctctgt gcggacggcc agtggcgaca gattgtcacc
     7741 attgaggacg ccctgaaagg tggctgcacg ctgttcgaca ttgagcagct caaacgcgaa
     7801 aacagcgccg acgattttaa aaacctgttc atgtgtgaat ttgttgacga caaggcgtcg
     7861 gtgttcccgt tcgaggagct gcaacgctgc atggtcgaca cgctggaaga atgggaagac
     7921 tatgcaccct ttgccgccaa tccgttcggc tcacgtccgg tatggattgg ttacgaaccg
     7981 tcacaccgtg gcgacagtgc cggatgcgtg gtgctggcac cgccggtggt ggccggtggc
     8041 aaattcagaa tacttgagcg tcaccagtgg aaaggcatgg actttgccac ccaggctgaa
     8101 tccatccgca aactcaccga aaaatacaac gtcgaataca tcggaattga tgccaccggc
     8161 ctcggtgtcg gcgtgttcca gctcgtgcgc tcgttctatc ccgccgcgcg cgatatccgc
     8221 tacacgccgg aaatgaaaac cgcaatggtg ctcaaggcaa aagacgttat ccgccgtggc
     8281 tgtctggaat atgacgtcag cgccaccgac atcaccagct cgtttatggc tatccgcaag
     8341 accatgacca gcagcggacg cagcgccacc tatgaggcca gccgcagcga ggaagccagc
     8401 cacgccgacc tcgcctgggc gaccatgcac gccctgttaa atgagccact caccgccggt
     8461 atcagcaccc cgctgacatc caccattctg gagttttact gatgagcaag aaaaaaggga
     8521 aaacaccgca acctgcggca aaaacaatga ccgccagcgc cccgaaaatg gaggcattca
     8581 cctttggcga gccggtgccg gtactcgacc gccgtgacat tctggattac gtcgaatgca
     8641 tcagtaacgg cagatggtat gagccgccgg tcagctttac cggtctggca aaaagtctgc
     8701 gtgctgccgt gcatcacagc tcaccgattt acgtcaaacg taatattctg gcttcaacgt
     8761 ttatcccgca cccgtggttt tcccagcagg atttcagccg ctttgtgctg gattttctgg
     8821 tgttcggtaa tgcgtttctg gaaaagcgct acagcaccac cggtaaggtc atcagactgg
     8881 aaacctcacc ggcgaaatat acccgtcgtg gcgtggaaga ggatgtttac tggtgggtgc
     8941 cgtccttcaa cgagccgaca gccttcgcgc ccggctccgt gtttcatctg ctggagccgg
     9001 atattaatca ggagctgtac ggcctgccgg aatatctcag cgcccttaac tctgcctggc
     9061 tgaatgagtc ggccacgctg ttccgccgca agtattacga aaacggcgct catgccggat
     9121 atatcatgta cgtcactgat gccgtgcagg atcgcaacga tatcgaaatg ctccgcgaaa
     9181 acatggtgaa gtcgaaaggc cgcaacaact ttaaaaatct gtttctctat gccccacagg
     9241 ggaaagccga cggcatcaaa attatcccgc tcagtgaagt ggcaacgaag gacgattttt
     9301 ttaatatcaa aaaagccagc gccgctgacc tgctggacgc gcaccgcatc ccctttcagt
     9361 tgatgggcgg caagccggag aacgtcgggt cactgggcga tattgagaaa gtggcaaagg
     9421 tctttgtccg caatgagctt atcccgttac aggataggat ccgcgagata aacggctggc
     9481 tcggtcagga ggtcatccgc tttaaaaact actcactgga cactgacaac ggctgaacat
     9541 cgccgcctgc gggcggcttt tttatatccc gtcatcacgc cctcacacgc tcaccaccgc
     9601 acaaaacacc ccgcagagac accaatgccc cggcgcacaa tctaaacgcc gtcacgacgc
     9661 gctcagacgc tgaaaaaata aaatccgcac caccgccagc gcgcagtgct ttccccgcct
     9721 cgcccgcccg cttcatgggt cggttttaat gcagttgcat aacatattat tcatagcacc
     9781 actgtgagtt tatggcttca ttttagcgag ttacttacgc atgcatttta atgcacataa
     9841 ttgcagttct agcctacgtt taactccagt gttaatcttt gcatatcacc atatctaatg
     9901 agattagagt tatgacagaa agccttgatt acatcgacct caataaactt gagcttgata
     9961 caaaaaatcc acgtcttcct gaaggtgttg aacgtacccc agaagcaatg ctaaatcata
    10021 tagctctaac aacctctatt gaagacttga tgaatgcaat cgcagaaaat ggattttttc
    10081 ctggcgagcc tcttattgcg gttaaagagg gagataagta caccgttgtg gaaggcaacc
    10141 gtcgcttaac ggcagttaaa cttattcata acccatatga gtgcgacaga cccagctccc
    10201 ggatgataga aatcgccgaa agcgctaaag ataaactagg tacactagaa aaattaccag
    10261 tgatcgttag agataccaga gctgaaattt taccttattt gggtttcaga catatcactg
    10321 gtgtcaaaca atgggaacct ctatcgaagg ctcgttatat cgagcaactt tttgggctca
    10381 cctccccaaa ctcaccgact aatgatcgat atcatcaggt tgctcgtgca attggtagca
    10441 gaaaagatca tatcaagaga aatctagatg ctttagccgt ttataaagta atggagagca
    10501 ataactttta tgatattgat ggattagatg aagaatcgat caagttttca attttatcta
    10561 cagccttagc tgacgagaaa attggattgt ttgttggcgt ctcagaaaaa gacgaatatg
    10621 gtgatattac atccaatgat gtaataattc acccacatca tataaataga gaaaatacca
    10681 gagaacttac actatggctt tataaaaagg atgattccgg gaaaacaaag gttggagagt
    10741 cccgaaattt acgcctatta tcttctgtta ttgataaccc caaggcttta acgtccttta
    10801 gaaacggggc tgaccttaag gttgcgtatc agttaactga agacttaaaa caagatttca
    10861 tgacgttact ttataaagct gaatctgcat taattgaagc ggctggaatt gttgcaacca
    10921 ttgattacaa tcctgaagca ttggaagttg caagacgcct aagtcaaaat gtcaaactta
    10981 ttggtaatac aattaaagcc aaaaaggttt ctgacgatga agatttttaa aatcggagac
    11041 attcatccta gtgttcctca tctttttgca gaccttgcag aacttgctac ggttataa
//
LOCUS       DAZUGK010000149        11034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZUGK010000149 DAZUGK010000000
VERSION     DAZUGK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.150"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(78..1727)
                     /gene="actP"
                     /locus_tag="ACHY5W_003452"
     CDS             complement(78..1727)
                     /gene="actP"
                     /locus_tag="ACHY5W_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFS4448287.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1724..2038)
                     /gene="yjcH"
                     /locus_tag="ACHY5W_003453"
     CDS             complement(1724..2038)
                     /gene="yjcH"
                     /locus_tag="ACHY5W_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFS4448288.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2239..4197)
                     /gene="acs"
                     /locus_tag="ACHY5W_003454"
     CDS             complement(2239..4197)
                     /gene="acs"
                     /locus_tag="ACHY5W_003454"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFS4448289.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4590..6026
                     /gene="nrfA"
                     /locus_tag="ACHY5W_003455"
     CDS             4590..6026
                     /gene="nrfA"
                     /locus_tag="ACHY5W_003455"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFS4448290.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQTRKNGLLSQ"
     gene            6071..6637
                     /gene="nrfB"
                     /locus_tag="ACHY5W_003456"
     CDS             6071..6637
                     /gene="nrfB"
                     /locus_tag="ACHY5W_003456"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFS4448291.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6634..7305
                     /gene="nrfC"
                     /locus_tag="ACHY5W_003457"
     CDS             6634..7305
                     /gene="nrfC"
                     /locus_tag="ACHY5W_003457"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFS4448292.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7302..8258
                     /gene="nrfD"
                     /locus_tag="ACHY5W_003458"
     CDS             7302..8258
                     /gene="nrfD"
                     /locus_tag="ACHY5W_003458"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFS4448293.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8278..9996
                     /gene="nrfE"
                     /locus_tag="ACHY5W_003459"
     CDS             8278..9996
                     /gene="nrfE"
                     /locus_tag="ACHY5W_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFS4448294.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVMLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKNGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9989..10372
                     /gene="nrfF"
                     /locus_tag="ACHY5W_003460"
     CDS             9989..10372
                     /gene="nrfF"
                     /locus_tag="ACHY5W_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFS4448295.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSKVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10369..10965
                     /gene="nrfG"
                     /locus_tag="ACHY5W_003461"
     CDS             10369..10965
                     /gene="nrfG"
                     /locus_tag="ACHY5W_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFS4448296.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMALNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         2472 a         2920 c         3088 g         2554 t
ORIGIN      
        1 ttaatattat gggaaaaggt gatgcatttg ggagaggaag agtattcccc ggtcagacga
       61 ccggggaagg ggagagatta atgcgcgcgg ccttgctcaa cgccaaagcc ggtctgggag
      121 cggataaact gcgcgcggaa cagttcacgc tcacgcgcgc cttccgctga gttatcggtt
      181 gccgagaaga accagatgcc gaggaatgcc acgctgatag agaacagcgc cgggtattca
      241 tacgggaaga tagccttttc gtgaccgagg atctgtaccc aaatcgtcgg gccgaggatc
      301 atcagtacca ctgcggtaat cagccccagc cagccaccca tcatcgcgcc acgcgtggtc
      361 agtttcgacc agtacataga aagcagaatg atcgggaagt tacagctcgc cgcgatggca
      421 aacgccagcc ccaccataaa ggcgatgttc tgattctcaa acagcacgcc gaggataatc
      481 gcaatcacgc ccaaaatcag gacggtgatt ttcgataccc gcagctcttc acgttcggtc
      541 gcaccttttt tgaaaacgtt agcgtacagg tcgtgagaca ccgccgatgc gcccgccagc
      601 gtcagacccg ctaccaccgc gaggatagtg gcgaaagcaa ccgctgaaat aaagccgagg
      661 aacaggttgc cgcccaccgc attggccagg tgaactgctg ccatgttgtt accaccaatc
      721 agatggcccg ccgcgtcttt atattccgga ttcgcaccga ccagcatgat cgcgccgaag
      781 ccgataataa aggtcaggat atagaagtag cccataaatc cggtagcgta gaacacgctc
      841 ttacgcgctt cgcgggcatc gctgaccgta aagaagcgca tcagaatgtg cggcaagccc
      901 gccgtaccaa acatcagtcc cagacccaga gagagcgcgg agatcggatc tttcaccagc
      961 ccgcccggct tcatgatgtc gacccctttc gggtgtaccg ccatcgcttc gctgaacaga
     1021 ttgttgaagc taaagccgac gtgtttcatc accataaagg ccataaagct ggcaccgaac
     1081 agcaacagaa cggctttaat aatttgtacc caagtggtcg ccagcatgcc gccgaacagg
     1141 acgtacatca tcatcagcac gccgaccagc accactgcaa tgtgatagtt aaggccaaac
     1201 agcagctcga tcagtttacc tgcgcccacc atctgggcga taaggtaaag cgccaccacc
     1261 accagagaac cacaggccga aagaatacga atcggccctt gtttcagacg ataagaggct
     1321 acatcggcaa aggtgtaacg ccccaggtta cgcagacgtt cggcaatcag gaacaaaatg
     1381 atcggccagc ccaccaggaa gcccagcgag taaattaagc catcatagcc ggaggtaaac
     1441 accagtgcgg agatccccaa aaatgaggcg gcggacatat agtcaccggc aatcgccagc
     1501 ccgttctgaa aaccggtgat attgccgcct gcggtgtagt agtcattacg agaacgtacg
     1561 cgttttgatg cccagtaggt aatgccgagc gtaaacacga cgaaaatcag gaacataata
     1621 atcgcctgcc agttcgttgg ctggcgctct acggccccgc taatagcatc cgcggcgtta
     1681 gctgcgaaag ggagtgtggc ggcaagcgcc gtcagaactc ttttcatgat gcttgtacct
     1741 catgcaggac ttcgttatta agacggtcga attcgccgtt cgcccgccag atgtagatac
     1801 cagtgagaac aaaggagatc acaatcacac caacaccgac tggaataccg cgtgtgacgc
     1861 tggtgttcgg attcagcggg gtgcccagcc agccgggcgc gaaggcgatc agtaaaataa
     1921 agccgatata aactgccagc ataataatcg acaggatggt ggcaaaccgt tgccgttttt
     1981 cgactaactc cctgaaatgc gcattgtctt ctatccgctg ataaatcgta ccattcatca
     2041 cagattctcc agaggtaatg tagggattgt tttaattccc gctcccttat gggagaaggt
     2101 taacgctcgg gtaacccttg ccgaatgtag gccggataag gcgttatacg ccgcatccgg
     2161 caatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc
     2221 gcatccggca attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt
     2281 ctcgactacg ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc
     2341 cgcaattttg cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc
     2401 ggtccagtgc agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg
     2461 gacttctgcg tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta
     2521 gatcgcctga cctttaatat tgtgcggaat accgacgacg gcggcttcgg caatcttcgg
     2581 atgcgccacc aacgccgact caatctccgc cgtccccaga cggtgaccgg agacgttcag
     2641 cacgtcgtcc acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc
     2701 gccgctgaaa tacatatttt tgaaggtgga gaagtaggtc tgctcaaaac gttcgtgatc
     2761 gccaaacagc gtacgcgcct gacccggcca ggagtcggtg atcaccagac tgccttcggt
     2821 ggccccttcc agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg
     2881 acgtgttgcc gaaccggctt tcagctcggt agcgccaggc agcggggtga tcatgaaacc
     2941 gccggtttcg gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt
     3001 ccagtaccac tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag
     3061 cgacgaacgg tcggtgcctt cgatcgcttt atcgccttcc gccatcagcg cgcgaatcgc
     3121 cgtgggcgcg gtatagagaa tattgacctg atgcttgtcc accacctgtg ccatacgggc
     3181 aggcgtcggc cagttcggta cgccttcaaa catcagcgtg gtcgcgccgc aggccagcgg
     3241 gccgtacagc aaataactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat
     3301 atcgcccgga tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc
     3361 gccggtagtg tgcagtacgc cttttggttt tccggtagaa ccggaggtat agagaataaa
     3421 cagcggatct tcggcgttca tctcttccgc ctggtgctga tcgctggctt gctcaaccag
     3481 gtcgtgccac cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag
     3541 taccaccaca tgctctacgc tggtgacgtt cgggtttttt agtgcgtcat caacgttttt
     3601 cttcagcgga atactacgcc cggcgcgcac gccttcgtcg gaagtgatca ccaaccgtga
     3661 gttagaatcg ataatgcgcc cggcaaccgc ttccggcgag aagccaccga aaattaccga
     3721 atgcaccgcg ccaatacggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg
     3781 catataaatc gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa
     3841 gcggcagacg tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc
     3901 gccttcccag atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt
     3961 tgccgccaga ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa
     4021 ggaggtgttt ttcaccttct ggtacggttt gatccagtcg agaatttttc cctgttcgcc
     4081 ccagaaggta tcaggtgcgt taatagattg ttgatacatc gcctcgtact gctgagggtt
     4141 tatcaggcaa cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt gactcatgct
     4201 tttgttctcc ttgtaggatg ttaataatat gtggcataag cgttaaatgt aggggtattg
     4261 gcagttttgt ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg
     4321 gcagggtaat ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt
     4381 tatgcattct gttgaaaaag aggaagatac tgactaactc taaagtggta ttttgcatgc
     4441 acttacaatt gattaaagac aacattcaca gtgtggttat ttgttacaca taggggcgag
     4501 caatgtcatg acagtgtagg tgcggttact gtcgtgaaaa acaataaaaa ccgccattgc
     4561 aacaatggcg caattcggat gaagccccta tgacaaggat aaaaataaac gcacgccgta
     4621 tcttcagctt attgattcct tttttctttt tcacttctgt tcacgctgaa caaacggctg
     4681 ctcccgcaaa acctgtaact gtggaagcga agaatgaaac ctttgccccg cagcatcccg
     4741 atcaatatct ctcctggaaa gccacctcgg aacagtcaga gcgtgttgac gccctggcgg
     4801 aagatccacg gctggtgatc ctgtgggcgg ggtatccctt ctcgcgcgat tacaacaagc
     4861 cgcgtggaca tgcctttgct gtgaccgatg tgcgtgaaac cctgcgtacc ggtgcgccga
     4921 aaaatgctga agatggtccg ctaccgatgg cgtgctggag ttgtaaaagc ccggatgtgg
     4981 cgcgtctgat ccagaaagac ggcgaagatg gctacttcca cggtaaatgg gcgcgcggcg
     5041 gcccggaaat cgtcaacaac ttaggttgtg ccgactgcca taacaccgcc tcaccagagt
     5101 tcgccaaagg caaaccggag ttaacccttt cccgtccgta tgcggctcgc gcgatggaag
     5161 ccattggtaa accttttgag aaagccggac gtttcgacca gcaatcgatg gtttgcggtc
     5221 agtgccatgt ggagtattac ttcgacggca aaaacaaagc ggttaaattc ccgtgggatg
     5281 acggcatgaa agtcgaaaat atggagcagt attacgacaa aattgccttc tctgactgga
     5341 ctaactccct gtcgaaaacg ccaatgctga aagcgcagca cccggaatat gaaacctgga
     5401 cagcgggcat tcacggtaaa aacaacgtga cctgtatcga ctgccatatg ccaaaagtgc
     5461 agaacgccga aggcaaactc tacaccgacc ataaaattgg taatccgttt gataacttcg
     5521 cccagacttg tgcgaactgc catacccagg ataaagctgc cttgcaaaaa gtggtcgcgg
     5581 aacgtaagca gtcgattaac gacctgaaaa tcaaggttga agatcaattg gttcacgctc
     5641 acttcgaagc gaaagcggcg ctggacgcag gcgcgacgga agccgaaatg aagccgattc
     5701 aggacgatat ccgtcatgcc cagtggcgtt gggatctggc gatcgcttcc cacggcattc
     5761 atatgcacgc accggaagaa ggtctgagga tgctcggtac ggcgatggat aaagcggcgg
     5821 atgcacgcac caaactggcg cgcctgctgg cgaccaaagg catcacccat gaaatccaga
     5881 tcccggatat ctcgaccaaa gagaaagccc agcaggccat tggcctgaac atggaacaaa
     5941 tcaaggccga gaagcaggac ttcatcaaaa cggtgatccc gcagtgggaa gagcagacac
     6001 gtaaaaacgg tctgttaagc caataacccc gttccgcctc gcaaggggcg gaaaacacaa
     6061 tggagtgaat atgagcgtat tacgttcgtt attaactgcc ggggtgctgg cgtcgggcct
     6121 gttgtggagc ctgaacggaa ttaccgctac ccctgcggcg caggcatctg atgatcgcta
     6181 cgaagttacc cagcagcgta acccggatgc cgcctgtctg gactgtcata agccagatac
     6241 cgaaggtatg catggcaaac atgcttccgt tatcaacccg aataacaaac tgccggtcac
     6301 ctgcaccaac tgtcacggcc agccatcacc gcaacaccgc gaaggggtga aagatgtgat
     6361 gcgctttaac gagccgatgt acaaggttgg agagcagaac agcgtctgta tgtcctgcca
     6421 tctgccggaa cagttgcaaa aagcgttctg gccgcacgat gtccacgtaa ccaaagtggc
     6481 gtgcgccagc tgccattccc tgcatccaca gcaagatacg atgcagacgt taagtgacaa
     6541 aggacggatt aagatttgcg tcgattgcca cagcgatcag cgcaccaatc cgaactttaa
     6601 cccggcgtct gttccgttgc ttaaggagca gccatgacct ggtctcgtcg ccagtttctc
     6661 accggcgtcg gcgtgctggc agccgtcagc ggcaccgcag gacgcgtggt ggcgaaaacg
     6721 ttgaatatca atggggtacg ttacggcatg gtgcatgacg agtcgttatg catcggctgt
     6781 accgcctgta tggatgcttg tcgggaagtg aacaaggtgc cggaaggcgt ctcgcgcctg
     6841 acgattattc gtagcgagcc gcagggcgaa tttcctgatg tgaaatatcg cttcttccgt
     6901 aagtcttgcc agcactgcga tcatgcgccg tgcgttgacg tctgcccgac cggtgcgtct
     6961 tttcgtgatg ccgctagcgg gattgtcgat gttaatcctg atctctgcgt cggttgtcag
     7021 tactgcatcg ccgcctgtcc gtaccgcgtg cgctttatcc atccggtcac gaagacggcg
     7081 gacaaatgcg atttctgccg taagaccaat ttgcaggccg gtaagctgcc cgcgtgcgtt
     7141 gaggcttgcc cgacgaaggc gctgacgttt ggcaatctgg acgatcctaa cagtgagatt
     7201 tcgcaactgc tgcgccagaa gcctacttac cgctacaagc tggcgctggg aaccaaaccg
     7261 aagctgtacc gcgtaccgtt taaatacggg gaggtgagcc aatgacgcag acttccgcat
     7321 ttcattttga atcgctggtg tgggactggc cgattgccat ctacctgttt ttgattggta
     7381 tttctgctgg tttggtgacg ctggctgtgc tgttacgtcg cttctacccg caggcgggcg
     7441 gtgcagacag cacgttgctg cgcaccacgc tgattgtcgg acctggcgcg gtgatcctcg
     7501 gtctgttgat cctcgtcttc cacctgacaa gaccgtggac cttctggaag ctgatgttcc
     7561 actacagttt tacctcggtg atgtcgatgg gggtgatgct gtttcagctt tacatggtgg
     7621 tgctggtgct gtggctggcg aaaatctttg aacatgattt gcttgccctg caacaacgct
     7681 ggttgccgaa gctggggatc gtgcaaaagg ttctgagcct gctgacgccc gttcatcgcg
     7741 gactggaaac attgatgctg gtgctggcgg tgttgttggg ggcttatacc ggttttctgc
     7801 tgtcggcgct gaaatcgtat ccgttcctta ataacccgat cctgccggtg ctgttcctct
     7861 tctccggcat ctcgtccggt gcggcggtgg cactgatcgc catggcgata cgccaacgca
     7921 gtaacccgca ttccacggaa gcgcagtttg tacaccgtat ggaaatcccc gtggtatggg
     7981 gtgaaatctt cctgctggtg gcgttttttg tcggtctggc gctgggcgat gacggtaaag
     8041 tgcgtgcgct ggtggcggca ttaggtggcg gtttctggac gtggtggttc tggcttggtg
     8101 tcgccgggct ggggctgatt gtgccaatgt tgctcaaacc gtgggtcaat cgcagttccg
     8161 gcattcctgc cgtgctggcg gcgtgtgggg ccagtctggt cggcgtgttg atgctgcgct
     8221 ttttcattct ctacgccggg cagttaacgg tggcgtaagc cagaacagag gtggtttctg
     8281 gacgtattcc ttcctgaagt cggttttctg gcgttgttgt taagtctcgg ggtcaacgtg
     8341 ttgaccccgt tgacggcctt cgcgggagtg cggttgcgct ggcctgccat gatgcgactc
     8401 acttgcatcg gcattctggc gcagttcgcg ctcctgctgc tcgcctttgg cgtactgacg
     8461 tattgttttc tcatcagcga tttctcggtc atttatgtcg cccaacatag ctacagcctg
     8521 ctgtcgtggg aactcaaact ggcggcggta tggggtggtc atgaaggttc gctgctgctt
     8581 tgggttctgc tgctttccgc ctggagcgcg ctgtttgcct ggcattatcg gcagcaaacc
     8641 gatccgctat ttccgctgac gttagccgtt ttatctctca tgctcgccgc actgctactg
     8701 tttgtagtgc tgtggtccga tcctttcgtg cggatatttc caccagcaat cgaaggccgc
     8761 gatctcaatc cgatgctgca acatcccggt cttatctttc atccaccgct gctttacctt
     8821 ggctatggcg gtttgatggt agcggcgagc gtggcgctgg cgagtctact gcgcggcgag
     8881 tttgatggtg cctgcgcccg aatttgctgg cgctgggcgt tacctggctg gagcgcatta
     8941 actgcgggga tcatcctcgg ttcctggtgg gcctattgcg aactgggctg gggcggctgg
     9001 tggttctggg atccggtgga aaacgcctct ttattaccct ggctttctgc cactgcgctg
     9061 ctgcacagtt tatccctgac acgccagcgg gggattttcc gccactggtc gctgttgttg
     9121 gcgatagtta ctctgatgct gtcattgctg ggcaccttaa ttgtccgttc tggcattctg
     9181 gtttcggttc atgcgttcgc gctggataac gtccgcgccg tgccgttgtt cagtctgttt
     9241 gtactgatta gccttgcgtc tctggctctg tatggctggc gagcgcggga cggtggcccg
     9301 gcggtgcgtt tttcggggtt atcgcgggaa atgttaatcc tcgctacgct gttgctgttt
     9361 tgcgcagtgc tactgatcgt gctggtggga acgctttatc cgatgattta cggcctgctg
     9421 ggctggggac gcctctccgt tggcgcaccg tattttaacc gcgcgacgtt accgtttggt
     9481 ctgttgatgt tggtggtgat tgtgctggcg acgtttgtct ctggcaaacg cgtgcagctt
     9541 ccggcgctgg tagctcatgc gggcgtgctg ttatttgccg ctggggtcgt ggtttccagc
     9601 gtcagccgtc aggaaatcag cctgaattta cagccgggcc agcaggtgat gctggcagga
     9661 tacaccttcc gttttgagcg cctcgatcta caagccaaag gcaattacac cagcgaaaaa
     9721 gcgatagtgg cgctgtttga ccatcagcaa cgcattggtg aattaacgcc ggagcggcgt
     9781 ttttacgaag cacgtcgtca gcaaatgatg gaaccgtcaa ttcgctggaa cggcatccat
     9841 gactggtatg cggtcatggg tgaaaaaaac ggagcggatc gttacgcttt tcgcttgtat
     9901 gtacaaagcg gtgtgcgctg gatctggggg ggaggattgt tgatgattgc gggcgcattg
     9961 ttaagcggat ggcgggggaa gaagcgcgat gtataaaggg cttctcacgc tactgctgtt
    10021 atttacctgt tttgcgcgcg ctcaggttgt agacacctgg caattcgcca atccgcaaca
    10081 acagcaacag gcgttaaata ttgccagcca gttacgttgt ccgcagtgcc agaatcaaaa
    10141 cttactggaa tccaacgcgc cggtggcagt cagtatgcgc catcaggttt acagcatggt
    10201 ggcggagggg aaaagtaaag tcgaaatcat tggctggatg accgaacgct acggagattt
    10261 tgttcgctat aacccaccgt taacgggtca gacgctggtg ttgtgggcgc tgccagtggt
    10321 gttgttactg ctgatggcac tgatcctctg gcgagtgagg gcgaagcgat gaaacagcct
    10381 caaataccgg tgaaaatgct gacaacactc acgatattga tggtattcct ctgtgtcggc
    10441 agttatctgt taagcccaaa atggcaggcg gtacgtgcgg agtaccagcg tcagcgcgat
    10501 ccgctacatc agtttgccag ccagcaaacc ccggaagcgc agcttcaggc attgcaggat
    10561 aaaatccgtg ctaatccaca aaacagcgaa caatgggcgt tactgggcga gtactatctg
    10621 tggcaaaacg attacagcaa ttcgctgctg gcgtaccgtc aggcgttgca actacgtggt
    10681 gagaacgctg aattgtatgc ggcgctggcg acggtgcttt attaccaggc cagccagcat
    10741 atgaccgccc agactcgcgc aatgatcgac aaagccctcg cgctggactc taacgaaatc
    10801 accgctctga tgctgctggc ttccgatgcg tttatgcagg cgaattacgc gcaagccatc
    10861 gaactatggc aaaaagtgat ggctctcaac tcaccccgga tcaaccggac acagctggtc
    10921 gagtcgatta atatggcgaa actgttgcag cggagatcgg attaatcatc tggtgatgaa
    10981 gattatgtta ttgagctgca taaaaaataa tcgaatgaac atatgccaaa aata
//
LOCUS       DAZUGK010000150        10967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZUGK010000150 DAZUGK010000000
VERSION     DAZUGK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.151"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            152..802
                     /gene="csgD"
                     /locus_tag="ACHY5W_003462"
     CDS             152..802
                     /gene="csgD"
                     /locus_tag="ACHY5W_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFS4448297.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            807..1196
                     /gene="csgE"
                     /locus_tag="ACHY5W_003463"
     CDS             807..1196
                     /gene="csgE"
                     /locus_tag="ACHY5W_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFS4448298.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHIVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1221..1637
                     /gene="csgF"
                     /locus_tag="ACHY5W_003464"
     CDS             1221..1637
                     /gene="csgF"
                     /locus_tag="ACHY5W_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFS4448299.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1664..2497
                     /gene="csgG"
                     /locus_tag="ACHY5W_003465"
     CDS             1664..2497
                     /gene="csgG"
                     /locus_tag="ACHY5W_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFS4448300.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2562..3053)
                     /gene="ycdZ"
                     /locus_tag="ACHY5W_003466"
     CDS             complement(2562..3053)
                     /gene="ycdZ"
                     /locus_tag="ACHY5W_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS4448301.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3155..3709)
                     /gene="ycdY"
                     /locus_tag="ACHY5W_003467"
     CDS             complement(3155..3709)
                     /gene="ycdY"
                     /locus_tag="ACHY5W_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFS4448302.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3733..4470)
                     /gene="ycdX"
                     /locus_tag="ACHY5W_003468"
     CDS             complement(3733..4470)
                     /gene="ycdX"
                     /locus_tag="ACHY5W_003468"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFS4448303.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4525..5463)
                     /gene="ghrA"
                     /locus_tag="ACHY5W_003469"
     CDS             complement(4525..5463)
                     /gene="ghrA"
                     /locus_tag="ACHY5W_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFS4448304.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESSLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5697..5784
                     /locus_tag="ACHY5W_003470"
     tRNA            5697..5784
                     /locus_tag="ACHY5W_003470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5731..5733,aa:Ser,seq:gga)
     gene            complement(5934..6776)
                     /locus_tag="ACHY5W_003471"
     CDS             complement(5934..6776)
                     /locus_tag="ACHY5W_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS4448305.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6861..7058)
                     /locus_tag="ACHY5W_003472"
     CDS             complement(6861..7058)
                     /locus_tag="ACHY5W_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS4448306.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7070..7561)
                     /locus_tag="ACHY5W_003473"
     CDS             complement(7070..7561)
                     /locus_tag="ACHY5W_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4448307.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7558..7932)
                     /locus_tag="ACHY5W_003474"
     CDS             complement(7558..7932)
                     /locus_tag="ACHY5W_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS4448308.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7979..8353)
                     /locus_tag="ACHY5W_003475"
     CDS             complement(7979..8353)
                     /locus_tag="ACHY5W_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS4448309.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8516..8737)
                     /locus_tag="ACHY5W_003476"
     CDS             complement(8516..8737)
                     /locus_tag="ACHY5W_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4448310.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8800..9276)
                     /locus_tag="ACHY5W_003477"
     CDS             complement(8800..9276)
                     /locus_tag="ACHY5W_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS4448311.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9292..9777)
                     /locus_tag="ACHY5W_003478"
     CDS             complement(9292..9777)
                     /locus_tag="ACHY5W_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4448312.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9868..10686)
                     /locus_tag="ACHY5W_003479"
     CDS             complement(9868..10686)
                     /locus_tag="ACHY5W_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS4448313.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AHQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2777 a         2739 c         2717 g         2734 t
ORIGIN      
        1 aatcagatgt aatccattag ttttatattt tacccattta gggctgattt attactacac
       61 acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca gctgtcaggt
      121 gtgcaatcaa taaaaaaagc ggggtttcat catgtttaat gaagtccata gtattcatgg
      181 tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acagctctct tgcagcacct
      241 taaacaatcg ctggcaatta caggaaaatt acataatatt caacgttctc tggacgatat
      301 ctcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc ttatccatta
      361 ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc taaatacgcc
      421 tgaagattac ccgtaccgcg atattgaaaa ctggcctcat atcaacggcg ttttttatgc
      481 catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgcg gcgagtgcta
      541 ctttacgcaa aagcttgcca gctacctgat tacacattcg ggtaactatc gttataacag
      601 cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg
      661 cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca
      721 tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg tttcctgggc
      781 aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt gtggcggcag
      841 aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg ggattgttaa
      901 ctgaccatat tgtttcatct attggccatg atttttaccg agcctttagt gataaatggg
      961 aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga tggggaagct
     1021 ggatcacaat aacggttaat caggatgtta ttttccagac ttttttattc ccgttgaaaa
     1081 gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc
     1141 gccagataaa tcaggcgtta ttaagtacgg gcgatttggc gcatgatgaa ttctaaataa
     1201 aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc atgcttattt
     1261 cgccattaag ttgggctgga accatgactt ttcagttccg taatccaaac tttggtggta
     1321 acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct tataaagatc
     1381 cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac tttactcagg
     1441 ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt aaaccgggcc
     1501 gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa ttgcagttga
     1561 acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt ttacaaaata
     1621 actcaaccga tttttaagcc ccagcctcat aaggaaaata atcatgcagc gcttatttct
     1681 tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag aagccgccag
     1741 accgacatta atgcctcgag ctcagagcta caaagatttg acccatctgc cagcgccgac
     1801 gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat ttaaacccta
     1861 cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc tggtcacggc
     1921 attgaaagat tctcgctggt ttataccgct ggagcgccag ggcttacaaa accttcttaa
     1981 cgagcgcaag attattcgtg cggcacaaga aaacggcacg gttgccatta ataaccgaat
     2041 cccgctgcaa tctttaacgg cggcaaatat catggttgaa ggttcgatta tcggttatga
     2101 aagcaacgtc aaatctggcg gggttggggc aagatatttt gggatcggtg ccgatacgca
     2161 ataccagctc gatcagattg ccgtgaacct gcgcgtcgtc aatgtgagta ccggtgagat
     2221 cctctcctcg gtgaacacca gtaagacgat actttcctat gaagttcagg ccggggtttt
     2281 ccgctttatt gactaccagc gcttgctgga aggggaagtg ggttacacct caaacgaacc
     2341 cgttatgctg tgcctgatgt cggctatcga aacaggggtc attttcctga ttaatgatgg
     2401 tatcgaccgt ggtctgtggg atttgcagaa caaagcagaa cggcagaatg acattctggt
     2461 taaataccgc catatgtcgg ttccaccaga atcctgacag ataaaaatgc cggaattaat
     2521 aatccggcat tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt atttttgatt
     2581 tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc gctgtttttc
     2641 attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt ccagtcaccc
     2701 tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga aagcagcagc
     2761 tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata accgagaatt
     2821 tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac cacgccacta
     2881 agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca agcaaagtag
     2941 gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc cacccatccc
     3001 cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt catgccgcgc
     3061 atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc aacaacaaat
     3121 tatccgttgc agtcatgagt gacgaagatc acatttactc ttcagaatcc tcttccagct
     3181 cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc cagaaaggcg
     3241 tggttgcatg ggcctccact ttgccaagga acgcgccaca ccagggtaac agatactcac
     3301 tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc agccaggaag
     3361 ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtggc atccctcgtt
     3421 cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg ctacgatatg
     3481 gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag acttgcgtca
     3541 tatcacaact tttctgtaaa cgcgtcagta actcatcctg ctccagtggc cagttcgcag
     3601 ccagtttccc ctcacgaatc agagtaaaca gcggcaccag taaaggatct tgtggttggc
     3661 ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt aatatatttc
     3721 cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc gagattcaag
     3781 gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc tctggcggaa aatctaccgc
     3841 gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt gagaatccga
     3901 gcctaatgcc acccaacctc cagcatcacg taccgctgcg gctaccgcac gacagttgtc
     3961 ttcactgccc ttgcgtgagt gtaaaaatga ggaattattg atttccagcg ccacctgatg
     4021 tttcgcggct gcttcagcaa cggctttaac atctatttca tatttcggat ttccgggatg
     4081 gcttattata tgcacattgc cgctggcgat agtggcgatc atcgcttgtg tattggtcgc
     4141 tttgtcatgt ggtgcaaaaa ccggctcatg aaaaccggca ataatcagat ccagcgagtc
     4201 aaacatttta ccgctgcaat caatttcacc atcaacattt ttaatgttag cttcgatgcc
     4261 gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa tgaagtgcca
     4321 gtgatgcggc gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa gtttaatgcc
     4381 cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg tgctggcaac
     4441 ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat tcccgccaag
     4501 gatagcagga atcctgatgc tttattagta gccgcgtgcg cggtcgactt gcccgcagac
     4561 cctctcccct ttttcgagct gggcaatagt gcgagaaatg tactccacag cttcagcggg
     4621 acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc agagcgagct
     4681 ttcaggcggt aagggttcac gattaaaaac atccagcatt gcgcctttaa ctttcccgct
     4741 atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca ggttgaggag
     4801 atacgcgcca tccggtaatt tttcgagtaa ttgttgatta ataatgccga cggtttcagg
     4861 agtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag acagttcttc
     4921 ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc agcaacgcag
     4981 cggaaagcgc caggtttgca gactctgagc aactttactg cccagcacgc ctgcgcccaa
     5041 aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat gcgaactatt
     5101 ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac tgacagcata
     5161 ttcctgcatt tgctcgccca taccggtatc ttccaagcga aaaagtggaa cggaaggctt
     5221 cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg ccccgagtgc
     5281 gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc agactaaagc
     5341 ataatcagca gagtcattat ctccgctttt ccatgctctg actcttgcct gaggaatagc
     5401 tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa agatgatatc
     5461 catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt atcatctaca
     5521 aagaccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt cgcttattaa
     5581 aaccacatat tgattgaagt ttgaataaac gcgcgatttt ttcaaaaagt ttgttgacct
     5641 caggtcatga tttccctaaa ttagcgcccg ttccagcaag acaggaacga caatttggtg
     5701 aggtgtccga gtggctgaag gagcacgcct ggaaagtgtg tatacggcaa cgtatcgggg
     5761 gttcgaatcc ccccctcacc gccagattat aagcgcatgt tattgtttaa catgcgcttt
     5821 tttattttcc cttccagaca gtatgccatc gggtatgcca tctgaaaccg gtttatatgc
     5881 cccgcgatca tatcttatcg gacaatttcc gatctttgag catactggca aagctatact
     5941 gccagcatat caggatagtg ttttgcgata atatcattca gtctgtcaac cagttctggt
     6001 tttctgaacg tgatatgcgc ggagcccttc ttaaagtatc tgatgctgaa catctcatct
     6061 tcatagctct ctttaccctg aacagactga atgtgatcat ccagacgaag ggtgatgttt
     6121 ccccggttgt cggggattgg tttaccgctg aacaggtgca acattctttc cagatcggcg
     6181 agtcggtcag cctgttgtcc ggtgataaga tgaaatcccc accggtccca tctcaccaga
     6241 ttgttgacga taattttact gccaaattta caggggcaat tggttttgta attccagttc
     6301 agccctctga agacgttgat cacccctcgc tcaaacacct catccttatt ctggtgcagt
     6361 tgttcaaatg tgctcagaat gttcgcttcg ctgatttccg gaaaattatc gtactccagt
     6421 gaccggtacc atgtatcacg ggcctgcgta tccattaatg acagcatccc ggaacgttgc
     6481 atcaggtcac gccagatttc gcgatcaaga ttccgggtaa tggctttcat tgctgtttcg
     6541 ggtttctcca tcagccagca gccgcagcga aaatcctgtt tcatggccca gtccggggca
     6601 gttttacctc cgatgctttc cgtcagcacc gagatttcag ccagttgtcg gataagtgtg
     6661 tcgatttgca acagtgtact ctttcgtaca gccagaatgt ggcgaatgct ggttgagcag
     6721 ataacgtccg tatgcccgtt gaccacttcc ggacgggaga ttgtgatatc tgacataatc
     6781 gatattcctt tttgcagata aatgaaaacg ccagccctgt gcaggctggc gtcagaggaa
     6841 tgaagtctgg tgagtgagct tcaccggttc tggtgcagaa gctgcagatg acgcagggta
     6901 cggacatcct cccgggcctg ttcgatacag ggcaacagtg ctgctgaatc cgttttatcc
     6961 tcatcgttgt caaagataat tcccgatccg cagtcgatat tgtcctgcag ccacgcaatc
     7021 agaatatcca gcgctgtttc cgtggttaat gatttcatgt tgttcctggt taccagtcga
     7081 aagtgggtaa acccggcagg cattcagcgc tggcatccag atgaatgaga ctgacgccat
     7141 aacgccggat gagtgtgacg accagacgac ggaacgtttt tgacagcccc aggcgtttaa
     7201 gccgcagaac cgggtatgac cacgcatcgg tacgtaacag atatccgcta cctgtaaaat
     7261 gaatccattc tgattcaccg aagtcactgg tctggtgtga cagcgagtac aaccaggcgt
     7321 tgtccgtttc cgtgatatgt gcggtactgc agcgtatgcc ggtaagggta gcaaatggtg
     7381 gcgtgcattc tgctgaggtc tggtcagatt catcaacaac acgaagggag tagccgttat
     7441 cgcagaccat gttaatcagt tcagcgagct caataccatc gacgtcaacg acaatgcgcc
     7501 ccatgttcag tgcctgtacg ttaacgctgt cggcttccag tgtcagggcc agtttcatca
     7561 tttcgcctcc ggatacttac ccaaggtaat gttatttacc gttctgtaat tgtcgcgggt
     7621 catcaggccg gtcgcccggc gagcccggag gatatcaata ctgtttatta actgagagcg
     7681 gggacaggtg ctgaatcccg gctggtcggt acgcaccagc gcgtattttt ccacgagaaa
     7741 attaactgca tcacacagtg aaatgcctgc ctcaatatgc tgctcaatca cacgttcatc
     7801 ggcaaacggt gtgtcattca gtgtgaggcc ataatgctgg tccagcagtc ggatgagcag
     7861 tgtctgccag atggtgacgg gagacgggca gcgagatacc tcccgtacat gtgtgtcgga
     7921 taatgttttc atcttaagtt ctctgtaact ggtagtgcta aaggtgagag cttcggggtt
     7981 atgaggtggt tgcgagtgct ggtgtcggat aaacagccag ataaacgtaa ccacaggagc
     8041 cgagggtgtc ggcttcgcag gtcagccctt ttgcattcag cgtgacggta tgctgatatc
     8101 ggggattcag ttcaccgctg gtgagcatga gttccagttg tttcatcagc agcggaaagg
     8161 cctggtccag atggtatgca tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg
     8221 caaggtaatg caggcggtta ccctcttgca ccagacgagc gccaaaacag ggcctcactg
     8281 tgcagggaag cccccaccag gggcggtcgt gattgtcatc gggatgagtt atcccggaga
     8341 gtttgtctga cacgataaaa tcctctctga aaagtggtta agaatggttt aacgatgaca
     8401 ggtcagccgg cgcgctgacc ggtttcggtg aagatgtatg cattgatgag aatggcctca
     8461 tccacagcgg cgtctgaggt catctggtcg tactcgtttc ccgggtgacg gaacatcagt
     8521 tcagggtgac actcatcagc cggacatacg ggccaaaact gtccttacgg cgttctgcaa
     8581 acacggcaag aacgccggga atatcctgca cttcacgacc agtatacgct tcagcactgc
     8641 cgtgccagcg atacttaccg gtacagaacg gaaaaagacg ggatgtcgga tgttgttggt
     8701 gaatacgcat ggcttcacca cgggtgataa ttttcataat gggatacctc tgaagacaga
     8761 agataaaagt gaaaacaggt gtgatgtggt tgtgaagggt taaagcagac catgttcggc
     8821 aaaggagaaa acctggttgc caccgactat cagatggtcc ggcacccgga tatccaccag
     8881 gcccagtgcc tgtaccagac gttcggtgat aagccggtct gccttactgg gtgtgacttc
     8941 accggacggg tgattgtgcg ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt
     9001 aatcacttcc cggggatgaa cttccgtacg gttgatggtg ccggtgaaga gggtttcacc
     9061 ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttccc gctccagtcc
     9121 cgccatgttc agaatcagcc attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg
     9181 ttcatgaaga tggcggtcca gggtttgcag ggcccgcaga atgagactgc gctcgccggg
     9241 cgtcatctct ccgggcagaa aggaaagctg ctgcattgtc cctgcctcca ttcagtcaat
     9301 gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca gccggtaata
     9361 gtggaccgtc attgcataac actccgtacg acaggcatga tgactgtacg tcatcagaca
     9421 ggcagcaata ccggcggctt ccgggctcat ttcagcgcgg ttgccgttca tggcattgaa
     9481 cagtacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg ccccgccgtt
     9541 gttcagggtg tacagattcc agataccacc gcagtagtct tcgcaaagac ggtccatcca
     9601 gccgaagaca cggggctcca gggtcaccca ctgtggaatg aggccaaaat gctgcggcca
     9661 gaagctgatg cgctgttcat cagggacggg agtggcaacc agctgaggtg ggttattccc
     9721 tgatgcagtg gttacggaaa cagaaggtgt ggtggaatta tgcgaaacgg ttgtcatgag
     9781 gttattcctt ataaaaagta aatgaatgga agaagccccg gggcaggaac agatgtgagt
     9841 caggactgca ctttcagtga aacggcatca gcgcatgctc tccagcagcg tttctgccat
     9901 cacccacaat gcgcgattga gcttaatgtc ggtatcaatg ctgtgaatgg cccgggtatg
     9961 gatacgtttt ccttttgcac tgcgaccgga aatcccgcct ttcagcatat tctcctggat
    10021 ggtctgatgt gcgctccaca ggtccttacc gtaatcctcc cggcgtcgcg gcgtcagaat
    10081 gtcggcggtg gtgacgggct gatgttcgtc accataacgg taagtcagtg ccgcctgtgc
    10141 cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc atggcatcac gcttctcctc
    10201 tatccggtca aaaacgccca ccacctcgta agccccttca atgactctgt ccaccacatt
    10261 tccccggtgt ggaacacgca attctcccag agactgaccg cagaccagtc cattggtaca
    10321 aatggcacga aaatatccgg gtaacatctg gtagctggat gaaccgtcat gggagttgag
    10381 cagaataatt tcagggacat gctgaccggt tatctgtccg gcccgccgca gacgcagcat
    10441 gtgttttgtg tattcccggc ggctctggtc gcgcacgcgg gtctggcagg caaagaacgg
    10501 ctgaaagcct tcctgctgca gattttccag gacggtgata gtgggaatgt acgcatagtg
    10561 ttcactgcga gaggtatgtc ggttttcccc aaaaatactc ggtacatggc gcatcagttc
    10621 ttcgtgtgtc agcggacgat cacgacgtat ctggttcgca taaccaaaac gactggctaa
    10681 tctcatgatt tgctccttat gaatgattaa gcgatataaa tgaaaaagcc gcgtctcccg
    10741 gagaagacgc ggcctgatgg gtgaaatgaa tgaggtttat tgtctgagaa gcccttcact
    10801 ggcgaaccac gtattaagct gtattccggc atcgccagtg caactgacct tcagcattgc
    10861 ggataacccg ccggaaatat gttcactggt tgtttttgta tcttccccca aaattaaata
    10921 aattcatctt aaccatcacc cagataatat atatctgaaa atatttt
//
LOCUS       DAZUGK010000151        10941 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZUGK010000151 DAZUGK010000000
VERSION     DAZUGK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.152"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..929
                     /gene="adiY"
                     /locus_tag="ACHY5W_003480"
     CDS             168..929
                     /gene="adiY"
                     /locus_tag="ACHY5W_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFS4448314.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1066..2403
                     /gene="adiC"
                     /locus_tag="ACHY5W_003481"
     CDS             1066..2403
                     /gene="adiC"
                     /locus_tag="ACHY5W_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFS4448315.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2507..4150
                     /gene="eptA"
                     /locus_tag="ACHY5W_003482"
     CDS             2507..4150
                     /gene="eptA"
                     /locus_tag="ACHY5W_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFS4448316.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNILVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTT
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4147..4815
                     /gene="basR"
                     /locus_tag="ACHY5W_003483"
     CDS             4147..4815
                     /gene="basR"
                     /locus_tag="ACHY5W_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFS4448317.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4816..5916
                     /gene="pmrB"
                     /locus_tag="ACHY5W_003484"
     CDS             4816..5916
                     /gene="pmrB"
                     /locus_tag="ACHY5W_003484"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFS4448318.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESTADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5892..5981)
                     /gene="pmrR"
                     /locus_tag="ACHY5W_003485"
     CDS             complement(5892..5981)
                     /gene="pmrR"
                     /locus_tag="ACHY5W_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFS4448319.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6093..7595)
                     /gene="proP"
                     /locus_tag="ACHY5W_003486"
     CDS             complement(6093..7595)
                     /gene="proP"
                     /locus_tag="ACHY5W_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFS4448320.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7859..8737)
                     /locus_tag="ACHY5W_003487"
     CDS             complement(7859..8737)
                     /locus_tag="ACHY5W_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFS4448321.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVLTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8734..>10941)
                     /gene="crfC"
                     /locus_tag="ACHY5W_003488"
     CDS             complement(8734..>10941)
                     /gene="crfC"
                     /locus_tag="ACHY5W_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFS4448322.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREFLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKAQLAALFQDDSEPLSE
                     IRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGAVPHFNTCQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2758 a         2853 c         2680 g         2650 t
ORIGIN      
        1 cctgtaaaaa aatatgtaca tgagaaatta ctataaaaat ttgtaatatt agtaaaactc
       61 gttattttta tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct
      121 ttgctttagc gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca
      181 gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga
      241 aagaacctat tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta
      301 tcgacgtctc ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag
      361 attacctccg gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta
      421 cgcccattct caccctgcca tgcctgacac cagacgtctt tcgtgttgcc gcgcaacaca
      481 gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcactg
      541 tgctatcccg ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta
      601 attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc
      661 ttagtatggt agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa
      721 gcgaaaacac cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg
      781 aattaatgat ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta
      841 cgtcgtactt tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta
      901 gtcagcacag agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata
      961 acggcatcag cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat
     1021 gacgtaattt attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct
     1081 gatgctcaca aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg
     1141 tcaggtgttt ttctgttacc tgcaaacctg gcctccactg gcgggattgc catttatgga
     1201 tggttggtga cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc
     1261 ctcgatccaa gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc
     1321 ggttatcaaa ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg
     1381 gtcattggcg taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta
     1441 accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa
     1501 atgatcaccc gcgtgcaggc cgttgccacc gtgctggcgc tgatccccat cgtcgggatt
     1561 gccgtatttg gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc
     1621 ggcctgggca ccttcggtgc gattcaaagt acccttaacg ttacgctgtg gtcgttcatc
     1681 ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct
     1741 atcgccacca ttggtggggt attgattgcc gccgtttgct atgtgctctc caccaccgcg
     1801 attatgggga tgatccctaa tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc
     1861 gcacggatgg cgttgggtga caccgccggg gccattgttt ccttctgcgc agctgcgggt
     1921 tgcttaggtt cactgggcgg ctggacattg ctggcgggtc aaacggcgaa agcggctgcc
     1981 gatgacggac tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtagcg
     2041 gggctgatta tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac
     2101 gcgaccaaag aattcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat
     2161 ctttacacct gtgcggcgtt actgctgctc ggacacggtc actttggtaa agcacgcccg
     2221 gcatatctgg cagttactac cattgccttc ctctactgca tctgggccgt ggtggggtcc
     2281 ggggcgaaag aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg
     2341 ctgaattaca accggctgca taaaaacccg tatcccttag atgcaccaat aagcaaagat
     2401 taattcccct taatccagca aagataaaag ccaaccttaa gaacttaagg ttggcttaat
     2461 tttgctttgc gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct
     2521 actaaaaaga ccctctttga atttactcgc ctggctattg ttagccgctt tttatatctc
     2581 tatctgcctg aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggactcgct
     2641 gcataacata ctggttttct tgtcgatgcc ggtcgtcgcc ttcagcgtga ttaatattgt
     2701 cctgacacta agctctttct tatggcttaa tcgaccgctg gcctgcctgt ttattctggt
     2761 tggcgcggct gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat
     2821 tgccaatatt attgatacca ctccggcaga aagttatgcg ctgatgacac cgcaaatgtt
     2881 attaacgctg ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga taaaaatcaa
     2941 acctgccacc tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt
     3001 actgctgatt ttgctggtcg ccgcactatt ttataaagac tacgcctcgt tgttccgcaa
     3061 caacaaagag ctggtgaagt ccttaagccc ctctaacagc attgttgcca gctggtcatg
     3121 gtactcccat cagcggctgg caaatctgcc gctggtgcga attggtgaag acgcgcaccg
     3181 caacccgtta atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac
     3241 ctcgcgggcg gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggttggc
     3301 gaaagataac gtggtctatt tccctaatac cacatcttgc ggcacggcaa cggcagtttc
     3361 agtaccgtgc atgttctcgg atatgccgcg tgagcattac aaagaagagc tggcacagca
     3421 ccaggaaggc gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa
     3481 cgatggtggc tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa
     3541 tctgcctggt cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga
     3601 agagtacatc aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca
     3661 cggtccgacc tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga
     3721 caccaatgag atccagacct gtagcaaaga gcaactggtg aacacttacg acaacactct
     3781 ggtttacgtc gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa
     3841 atttaccacc agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat
     3901 ctatctgcac ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccaatgct
     3961 gctgtggctg tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa
     4021 acaggcgcaa acgcaacact attcacaaga caatttattc tccacgctat tgggattaac
     4081 tggcgttgag acgaagtatt accaggctgc ggatgatatt ctgcaaactt gcaggagagt
     4141 gagtgaatga aaattctgat tgttgaagac gatacgctgt tattgcaggg actgattctg
     4201 gcggcgcaaa ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa
     4261 agccttgagg caggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat
     4321 ggactgcatt ttctcgcccg tatccggcag aaaaaatata ccctgccggt actgatcctc
     4381 accgctcgcg atacgctgac cgacaaaatc gccgggctgg atgtcggtgc cgatgactat
     4441 ctggtgaagc cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc
     4501 cataataatc agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc
     4561 cgtcaggtat ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg
     4621 tcacggttaa tgctcaaagc aggcagtccg gtgcatcggg aaattctcta caacgacatc
     4681 tataactggg acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc
     4741 gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gcttgtcgcg
     4801 aatgaggaaa actaattgaa tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa
     4861 cggctgatat tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg
     4921 ctatggcatg aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc
     4981 aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc
     5041 ggcgtcttta tggtcagcct gacgctattt atctgctatc aggcggtacg ccgcatcacc
     5101 cgcccgctgg cggagctgca aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc
     5161 attgccattc acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt aaacgatctg
     5221 gtcagtcgcc tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac
     5281 gaactgcgaa cgccactggc gggggtacgt ttgcatctgg aactgctggc gaaaacgcat
     5341 cacattgatg tagcaccgtt agtggcacgg cttgatcaga tgatggagag cgtctcccag
     5401 ctgctgcaac tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa
     5461 ctgctggaag atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga
     5521 cagcaaaccc tgctattgcc agagagcacc gccgacatca ccgttcaggg tgatgcaacc
     5581 ctgctgcgga tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc
     5641 agcaacatta tgattaagct gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa
     5701 ggaccaggta ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgaatggac
     5761 agccgttatg gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat
     5821 cacgggcagt ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg
     5881 ctgaagaaag atcagtacgt ggcaaaccag atataaagaa agctgctgac caccagcacg
     5941 ctgaacacgg tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac
     6001 ctggaggaga gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata
     6061 agacagcgtt acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc
     6121 accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc
     6181 tgctcgatat tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc
     6241 gccggtgttg cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt
     6301 aaaccaacca ccgccactac catcaggtaa taggctggca tcatcagatt ctgcgagctt
     6361 tcgaccagcc aggccgccaa tgttggcgtc agaccggcaa ccagcaccga aatattaaat
     6421 gccgccgcca gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc
     6481 ataacgcccg taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcaga
     6541 ccgatgacgt tactgttaat cagaataaac gccgggatcg ccaacacaaa cagggcaaca
     6601 ctaccaagta gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc
     6661 tgaacaaaca gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag
     6721 taatgcaggt tatgtgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg
     6781 ttggtggcaa ttaccagacc aatacatgtc aacaggctgc gccagtgttt ggtggcaatc
     6841 tctttaaacg agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta
     6901 tcgacatgct gctggaacgc cggagtctct tccagcgcat ggcgcaggta aagcccgata
     6961 atccctaacg gcagagcgat aaagaacgga atacgccagc cccaatcgag gaagttctct
     7021 tcgccgacaa tggtcgaaat taacaccacc acgcccgcac ccagcacaaa cccggcaata
     7081 gaaccaaagt ccagccagct gcccataaag ccacgtttac ggtcagggga gtattccgca
     7141 acaaatatcg acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag
     7201 atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata
     7261 cagaacgtac tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat
     7321 ttatcgccca acataccaaa gaagagtccg ccaagcggtc gaatcagaaa gggaacggag
     7381 aaagtggcaa gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta
     7441 cctaatgcgt aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc
     7501 agtgatgctg cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga
     7561 agggtaatcg gttttacttt tttccttttc agcatagctt tcctcgcaga gttggcccat
     7621 ttccgcaaac gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga
     7681 tgcaatctct gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa
     7741 tcaaaccagt tgacgcctga attgggcagt taatgacctt tttaccctac cagcgtttaa
     7801 atttgtgatc aacttcacac atgaatttcg tttaggactc attgatgtaa ctgattattt
     7861 acaccaaatt tacagagaag atttcctcga gcattgcccc cgccagacgc tgcgctgaac
     7921 agagtcgtgg catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg
     7981 gttgtttatc tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc
     8041 ccgtcagaac ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggggccg
     8101 cattgatact ctctgccaga tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg
     8161 ccaccgcttg cgggcttccc agacgacagg catcgccaaa tgccccccaa cgcaactctt
     8221 cgagaacgat aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc
     8281 gggtaaaaat ttgctcgctc tgttcacccg cctgcaccag taggcctcgt tgcgttaacg
     8341 ccttttcctt ccagcgacaa cgggctgaaa accactcata caaggcggca atttgctgag
     8401 ggagctccgg gaaagcagag tgctggcggt gatccatttt ttgtaacagc gccagatgtt
     8461 gcgcgatctg cgtcagcgcc agatgaccgg gatccagcat cgacgccagt ttttccacca
     8521 atgtcagacg caggctaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct
     8581 gcacgcgcgc aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg
     8641 gaaggcgggc atcatcgccc tgcgccagat ccactaaaaa gcggggataa accgactcca
     8701 gcacgcgacc ggggccgtca agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt
     8761 ttgaatatca tcgcgtaaca gctgggcgtc ggtgtaaatc cagccgtgag tcatcacagt
     8821 ctgctgcaat tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag
     8881 actttcgcgt aaacgcgtct gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt
     8941 attcatgcct gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg
     9001 gcaatagaga tcgacttctg cgcttaatgc tcgttgcaac acactgtaat caaccgtttc
     9061 tgtcggggat ttctcatttc cccgtcccca gtcaggctga ttcaaccagc gcgaaaaagt
     9121 ctcacgcaca acgcctaaac gcgtgctctg ctcgtccgtt gcatcctggc gcgaaatgac
     9181 ttcactgaac agctggcaag tgttgaagtg gggaactgcg ccgtgaaaaa caggaaaatg
     9241 aaacccagga cgaaaccctg actcgctcaa ttccattttg acttgttgtt caatggggcg
     9301 aataacatcg gttaacaccc ggcaaagagt ggattcaagc tcggcaaaac gcagcgtaaa
     9361 gtcgcgactg atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgaatctc
     9421 gcttaacggc tccgaatcat cctgaaacaa ggcggctaac tgcgccttca gcgcatcttg
     9481 ctgttgacgc agaaagtggt tggcggaggt cagggccagc tcgatttcat gtttaatctc
     9541 gccgctcacc tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat
     9601 attttgccgc aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggaattc
     9661 ccgcgcctgc tgcgcgtaat tcaacagttt atgcgcggca gatcgcagag catacaacga
     9721 ggcgttagcg taagcggcat gaagcaacat ctgaattggc tgggcgaaca gcgaatcttc
     9781 ccacaactga tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca
     9841 gcgcctgccg agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcggtgg
     9901 taacttaccg ttgttggcta actcatggcg cgcccgattt gccaggtagc cccacatcga
     9961 cgacaccgga aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag
    10021 tgcccgcacc tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag
    10081 cacatacagc ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga
    10141 gatcgatttc agttgcgtat aatccagcac cgccagtacc gccgaggcgc gagccagctg
    10201 ctggttaagc attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag
    10261 taacgtcaac tgaccgggat aactctccag ccccgccaga tggacaaact ccacttcaat
    10321 cacgggaata tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt
    10381 cgccagtcgc actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag
    10441 ataatatttt tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt
    10501 atctatttcc agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa
    10561 tttttgaatt aaacaatcga ttggtgcgac atgtgaaaaa tgcagtaccg gttctttttg
    10621 cccgggcgta tggcgaataa gcgtcggcag cgcagtcatt gggcgattgc gattaggcag
    10681 tacttccgta ccaacaatgg cattaatggt ggttgatttc cctgctttca tagtaccgac
    10741 aatcgccagc accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg
    10801 acgctcggcg atattaggct gactccaggg taaagccagt tgtggcgcat cgtctccggg
    10861 tacagagaga ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacagccg
    10921 ttcagcctcc tgacttaact c
//
LOCUS       DAZUGK010000152        10931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZUGK010000152 DAZUGK010000000
VERSION     DAZUGK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.153"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..159)
                     /locus_tag="ACHY5W_003489"
     CDS             complement(<1..159)
                     /locus_tag="ACHY5W_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFS4448323.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTII"
     gene            403..660
                     /gene="ynaJ"
                     /locus_tag="ACHY5W_003490"
     CDS             403..660
                     /gene="ynaJ"
                     /locus_tag="ACHY5W_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFS4448324.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(710..1660)
                     /gene="uspE"
                     /locus_tag="ACHY5W_003491"
     CDS             complement(710..1660)
                     /gene="uspE"
                     /locus_tag="ACHY5W_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFS4448325.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIYEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1812..2564)
                     /gene="fnr"
                     /locus_tag="ACHY5W_003492"
     CDS             complement(1812..2564)
                     /gene="fnr"
                     /locus_tag="ACHY5W_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFS4448326.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2759..3274)
                     /gene="ogt"
                     /locus_tag="ACHY5W_003493"
     CDS             complement(2759..3274)
                     /gene="ogt"
                     /locus_tag="ACHY5W_003493"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4448327.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3285..4811)
                     /gene="abgT"
                     /locus_tag="ACHY5W_003494"
     CDS             complement(3285..4811)
                     /gene="abgT"
                     /locus_tag="ACHY5W_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFS4448328.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(4848..6293)
                     /gene="abgB"
                     /locus_tag="ACHY5W_003495"
     CDS             complement(4848..6293)
                     /gene="abgB"
                     /locus_tag="ACHY5W_003495"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFS4448329.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIVLLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFTVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(6293..7603)
                     /gene="abgA"
                     /locus_tag="ACHY5W_003496"
     CDS             complement(6293..7603)
                     /gene="abgA"
                     /locus_tag="ACHY5W_003496"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFS4448330.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHILKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQHVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            7779..8687
                     /gene="abgR"
                     /locus_tag="ACHY5W_003497"
     CDS             7779..8687
                     /gene="abgR"
                     /locus_tag="ACHY5W_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4448331.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYQQS"
     gene            9017..9580
                     /gene="smrA"
                     /locus_tag="ACHY5W_003498"
     CDS             9017..9580
                     /gene="smrA"
                     /locus_tag="ACHY5W_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFS4448332.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(9601..10833)
                     /gene="dgcM"
                     /locus_tag="ACHY5W_003499"
     CDS             complement(9601..10833)
                     /gene="dgcM"
                     /locus_tag="ACHY5W_003499"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFS4448333.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVILLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         2801 a         2699 c         2697 g         2734 t
ORIGIN      
        1 aattatggtg tagattaaaa attgcaccgc atggaataaa aaaccttttc ttttacgatt
       61 tccgcggcga aaccaaaagc tcatcagaat caatgctgcg cagctaccga aaataatgac
      121 cagattaagc gcattatttg taaacagttc agcgatcatt gttttatcag gctcctccag
      181 ataattgtcg tcatgccgga aacccctggc ggtgctattt taccgcgaca attcattcag
      241 atcatcaata gtcagggaag gaagtagcaa cattagctaa ctaatcaatg cgttgattgt
      301 aaatccagct aagaggtgag gttttcagag cagacaacgg tgaaatgtca tggtattgtt
      361 acgtttaggt aacaagaaat ttgtctgcac aaggattaca tcatgattat ggcgaaactg
      421 aagtcagcga aagggaagaa atttctcttt ggtttgttgg cggttttcat tattgcggcg
      481 tcggttgtga ctcgcgcgac tatcggcggc gttatagaac agtacaatat tccgctgtct
      541 gagtggacga catcaatgta tgtgattcag tcatcgatga tttttgttta tagcctggtc
      601 tttactgtgt tgctggcaat cccgttggga atttatttcc ttggcggcga agagcagtaa
      661 gtaaaaaata ggcccgataa ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt
      721 cttcgtcatc cagttcaacg ggtgtctgat actggtcagg tttaatgacc aacagatcgc
      781 agcgaagatg atcaatcacc tgttccgccg tgttgccgag gaatgctgct gaaataccgg
      841 tgcgtcctac cgtgcccaga accacaatcc ccgcctgtaa gtgctccgcc aaatcaggaa
      901 tcacctcttc tggcagacct ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct
      961 gccgcagagc tttcattgcc agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg
     1021 gatcaaattc cggcagttca atcgcgatat taattggcgt tactgggtaa gcgccaacca
     1081 gatgaacttc ggtatggttg acttgttctg ccagttcgat cgtctctttg accagttttt
     1141 cattgagcgc attatgatac ggctcttcac tggcgagatt caccgccacc agcgccttac
     1201 ctccttccgg ccacggctgg tctttcacca tccacaccgg gcttgggcat ttgcgtaaca
     1261 gatgccagtc cgttggcgta aaaatcaccg cttccagacg gtcatgttgg tgcgccattt
     1321 ttagcaccaa atcgtgcccg ccgctgatca cttcctgaat gatggcttcg aaaggacggt
     1381 tatgccagac cactttaatt tcaatgggaa cgccagcatt gagataatat tttgcctgct
     1441 cgtagatcca ggctgtacgc tggctgatga cgccctgacg catagcggta cgttcgtccg
     1501 gggagagcag ggtggtcatt tcgtatgaga agtcatagat cggcaaaaag gctttaattt
     1561 tgccaccaat ccgttgatgt aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt
     1621 taggatcgat aacaacgagc atgttctgat acatagccat acagggtctc cttacaacaa
     1681 ctgtcaacgc agtttgtaat taaaagatta acccatatct ggtgaatgaa acagtgatga
     1741 accttctgcc agatcaataa atcagaaaaa tttaatgata tgacagaagg atagtgagtt
     1801 atgcggaaaa atcaggcaac gttacgcgta tgaccagcaa gctgggccag cgcatcgtta
     1861 ttttcgatgg tgatgtattt acctttgact gccagcatgc cgcttttctg gaagcgaccc
     1921 agcagacggc tgatggtttc taccgtcagg cccagatagt taccgatatc gccacgagtc
     1981 atcgtcaggc ggaattcacg aggggagaag ccgcgttggg caaaacgacg ggacaggttg
     2041 tagatgaatg cagccagacg ttcctcggca tttttctttg acaacagcag gatcatgtcc
     2101 tgatcgcctt tgatttcacc gctcatcaga cgcatcatct gctgacgcag attcggcatt
     2161 ttaccggaca aatcgtccag cgtttcgaac gggatttcac ataccatcga ggtttccagc
     2221 gcctgcgcga agctcgggtg atggccgctg ccgatggcgt caaatcccac caggtcgcct
     2281 gctaaatgga aaccagtgat ttgctcgtcg ccttgctcag tgatggtata acttttaatc
     2341 gtaccggagc ggatggcata aagcgattta agttcatcac cagccttaaa cagcgtctgg
     2401 cctttctgaa taggcttctt ccgctcaatg atattatcaa gctgatcaag ctcatgttcg
     2461 ttgagtgtga acgggatgca aagctggctg atgctgcaat cctggcaatg gatagcacaa
     2521 ccgccagact gaatgcgccg tataattcgc ttttccggga tcataggtct gctcaagccg
     2581 taattgatat ttgtcaattt taacatcttt ttagggagca agtaagtcta agcaaacctt
     2641 aacagcagag aattccgata ttagatgtaa atatatgtct atctatttga aaacccttaa
     2701 gttgttaagg gtaactttac ataaaagtgt gaacaagctg gcacaaatta tttaatgttt
     2761 acagcaaaag ataaccttca tggcgcaata accactcttt tcgctgaact ccgcctgcat
     2821 atccggtcat ggtgccgttt cggccaataa cccgatggca aggtacgacg atgctgatgg
     2881 gattcgatcc gtttgccgct ccaacggcac gcgccgcgcc agggcggccc aattgttcag
     2941 ccagttggcc gtaatgcatt acctgcccgc aggggatagt gcgtagtgtt ttccagactt
     3001 cgcgctgaaa tggcgtcccc cctgtggcag tgggaagcgt atcaataatg ctaagattac
     3061 cggcaaaata ttcacgaagc ttgtcgctta aaccgcctgg attggtggca gaaatgcgct
     3121 catagccttc tttgcgataa tggatgtcca gcagctgcac catgcgttcg ctgtactctt
     3181 cccattcaac cgcccgcagg cgaaattgct catcgcaaat cacccacagt ggacccagtg
     3241 gtgtggcaat tttttcttca agtaatctca gcatccgttc tctcttaaga caaacgtggg
     3301 taaatccccg gacctatcgg caggccaaca agataccacg ccagcaacat cagcagccat
     3361 accaccaaaa atataagtgg atagggcaag actaacgaat agtaagtacc cagtttcgcg
     3421 tctggtttgt agcgttgcag gaatccaaga aacagtggaa caaaaggaga taccggcgct
     3481 aaaggcaata cggatgagtc ggcaatacga aagaggattt gcgcaaatgc cgggtgaaag
     3541 ccaagtagca taaacattgg tacgaaaatg ggggccagaa tcgaccagat tgcggaaccg
     3601 ctggcaataa acatgcataa gaaagaggaa agcaacgcca ggccgacaaa cgccgggatg
     3661 ccgctaagcc ctgaactttc cagtatatcg gtcagcccca cggcgatgaa tttccccatg
     3721 ttgctccagt taaacatggc gacaaactgg gcgaggggaa aaaccatcac gataaatccc
     3781 gccatctctt tcatcggttc aatcattaaa tgcggtaaat ccgcctgacg tcgaattgtg
     3841 cgggtagcga tgccataagc cagcgagaca acaaagaaaa aaagaatgat cagtggcacg
     3901 atacctttaa taaagggtga tggcatcacg gtgtgattaa tcggatcgcg caatatcccg
     3961 ttttccggga tcaccatcag cgcaatcgca gcaataaaaa gtagcgatac gacacctgct
     4021 atgcgtaaac caaaacgctg actttcggtc aatgtctgca gtttctcatc gctgtttccc
     4081 tgccattgac ctaaccgtgg ctcgatgatt ttgtcggtta tcaggccgcc aacaatcgtc
     4141 agtacgacta cggagctggc cataaaatac cagttatcaa ttacactgac gtgcatttgc
     4201 ggattgaacg cagccgccgc ttccgtgctg atccccgaca gcaacacgtc ggttgtgaca
     4261 atcagtaaat tagccgtaaa gccgcaacct acgcctgcaa tggcagccag taaacctgca
     4321 actggatgcc tgcccaccgc cagaaaaatc agcgcaccca tcggtggcat gatcactaac
     4381 gccgcatcgg aagaaatgtg gctgaaaaaa gcaataaaca gcaccatata actggcgtag
     4441 cgggcattaa catgcgatgc cattttaacc attagcgctg gcagtaagcc gacgcgctcc
     4501 gccagaccgg cacctaaaac cagcgccagg atcgcaccaa gtggagcaaa accgctaaag
     4561 tttttaataa cattgggtaa aaaccagtgt aatccttcca cactgagcag gtttttcacc
     4621 accaccggcg taccatcggt cgggtttttc gcactgacgc caaaggccga caaaattgcc
     4681 gtcgtcacca tgagtacgat aatcaaatag ataaagagca aaaaaggatg gggaaccttg
     4741 ttaccaattc tttcgaccca gccatagagc ttcccggatt gggaggacga cggtatggat
     4801 gacatactca tgggcattcc tcggttgttg tgtttgcgtt gttgttgtta ttttaaaggt
     4861 gacggtgtca cgtttttcgg gatagggcag tgatacggtt gcgtgtccgt tacttgctga
     4921 tgctcttgtt ggcattcttg caatagccct gaatcaagga agagattgac tgtggttgct
     4981 gccatagttt tcgccgccag cagcattcct ttatgagcaa tagatgttcg cccctggcta
     5041 accagttgcc acgtatgtag cggtgtaccg acggtaaaac aggggctgaa acactgggca
     5101 acaggcagtt tccaactgac gtcgccgaca tcagtcgatg ccgcaagcac gttatcggtg
     5161 gcggcatatg gagcgacttc attcgccagt accgtttcac gatgacgtag tgcaaaagcc
     5221 ttgccgtttt cgccaccggt tgcggcgata ttattcagac tgttttgccg atcgttgggg
     5281 gtgagcgtag cctgaatttg tttcgcaaaa gccagttctt ctgagttcca ttccggggta
     5341 ccaaaatggg atagggcctg gtacatggca ttttctaagg tgcgattcgg gagataactg
     5401 gaacaggctt tgtcgaagcg gcattcaacc gtggtttcgg tcatcaatgc cgcaccttcg
     5461 gcgattttgg cgactcgatc ataaatatgc tgcacgtcgg tcatttcggg ggcgcggata
     5521 agataaagga cttctgcctg cgcctggacc acgttgggcg agatcccgcc actatttgtg
     5581 atggcatagt gtacgcgcgc tttttcaata atatgttcgt tgaggaagtt ggtgccagtg
     5641 gtcatcaacg ttacggcatc aagggcgctg cgtcccaaat gaggggaatt cgcggcatgt
     5701 gctgcgatcc ctttaaagcg ccatgatgcc tgaatgtttg ccagcgtgcg ggtattgaac
     5761 ataccggcaa aggcttccgg gtgccaggta agtgccgcat ccacatcatc aaataccccc
     5821 tcgcgaacca tgaacgtttt acccgagccg ccttcttcgc caggacaacc ataaaagcgc
     5881 accgtgccgc cttgcccata ttgttccagc catttcttga cggctattgc agcggcaaag
     5941 gcggcggttc ccagcaaatt gtgtccgcaa ccgtgaccat tttcaccggg cgtcgcggat
     6001 gtaggttgcg cgcaaccagc ttgctgactt aaacctgcca gggcgtcata ttctcccagc
     6061 aggacgataa ccggtttgcc ttgaccaaac gaagcaataa aggcatttgg gatattgcct
     6121 acgttgcggg taacggtgaa gcctgcagat tccagcgccg aagccagatg ctccgctgac
     6181 cagaactctt caaaacgtgt ttctggatga tcccagattt gatcggcaat atcggtataa
     6241 cgttggcgat cggcttcaat cgcatcgtcg ataaaacgat agatttcctg catcagatac
     6301 ctcgcgtcca gggaaaattg agcgcggtgc gcgccagcgt ttcgacggca atagcgagaa
     6361 cctgctcgtc aaaatcgaat ttttcgttgt gatgacctgc cgccagctgt gtgccaaaca
     6421 ccatgtagga ggcttgccct tgatgttgct gcacgcgggc catcattaat gtggcatctt
     6481 cggaacccgc aggcgcttca acacgttcaa tggcctgatt gacccccgcg acctgagccg
     6541 cctgactttg caaccatgcg acccattgcg gcgaaggaga actggcggta gctgcaccca
     6601 tcagacgagt ttcaacgccg acaccataca tggttgctgc gccctgaatc gcttgctgag
     6661 cacggtcaaa aacatgttga ttaatgacgt cgctggcccc gcgtgtttcc actttcagca
     6721 acgccgaggc aggaacaaca ttacgaccgc ttcctgcctg cataacgccc acgtttactc
     6781 tggaagcgcc ttcgctgtgc ggggcgattg catgtagtgc aagagttgct tgtgctgccg
     6841 ccaacaaggc attgtgaccg tcttctggtt ttgcgcctgc gtgagcggcg gtcccggtga
     6901 agtgcgcgtc aaatttggtg gttgccataa aattatcact gccgcacacc acggtgcccg
     6961 caggtacgcc agtgccaatg tgcacggcag taaaataatc aacatcatcc actacacctg
     7021 catcgaccat cgcccgcgcg ccacgcgtac cttcctctgc aggctgaaaa atcagtttga
     7081 tgacgccatg tagtccggac tcgaactgtt taaggatatg cgccagccca agcccaatgg
     7141 cggtatgtcc atcatgacca caggcatgca tcattccggc gttacatgac gcaaaaccgt
     7201 cgcggtaggg gcgatggctg acatcctgct cttcactgag atccagcgcg tccatatcga
     7261 cacggaaagc catcaccgga ccggggcgac cggtatccag ggtggcgacg ataccagtga
     7321 aaccaccttc aaaagccgca atccattgtg ctagcgcacc ctgttgacga gcgcgctcga
     7381 attcgcgttg tagagtgaat tcatcaggta atcccatccg gctactttca ttaactactt
     7441 cgcgacccag cgccagtgaa tagccgagct ggtgcaattc ttccgcaaca agggtggcag
     7501 tgcggaattc cacccagcca gactctgcat agtgatgaaa atcacgtcgc cagtgcgata
     7561 attttggggc aagcgaatta acaaattgat tcaaagactc cataaccttt cccgtcatca
     7621 gtaaaaagtg tgacccggtt cacgtagcga tagtttttac ttatcactaa ctgatttttc
     7681 acagttttaa ccgttcataa attaccctga cacaatcatc tgcattaaag tagatgccag
     7741 tttctttggt ctgataaata acggttatcg gtggcgtcat ggcttttcag gtaaaaattc
     7801 atcaaattcg ggcttttgtt gaagtggctc gtcagggcag cattcgcgga gcgagccgaa
     7861 tgttgaatat gtcgcaaccg gcactgagta aatctattca ggagctagaa gaagggttag
     7921 cggcgcaact cttttttcgc cgtagtaaag gcgtgacgtt aactgatgcc ggtgaaagtt
     7981 tttatcagca cgccagtctt attcttgaag agctgcgcgc agcccaagag gatattcgcc
     8041 aacgacaagg gcaactggca gggcagatta atatcggcat gggggccagt atttcccgca
     8101 gtctgatgcc agctgtcata tctcgttttc atcagcagca tccgcaggta aaagtacgca
     8161 ttatggaagg gcaactggtg tcgatgatta atgaattgcg tcagggagaa ttggatttca
     8221 ccatcaatac ctattatcag ggaccgtacg accacgaatt tacttttgag aaattactgg
     8281 aaaagcaatt cgcgatcttt tgccgcccgg gacaccccgc cattggtgcc cgttcgatca
     8341 aacagttact ggattacagc tggacaatgc cgacgccaca cggcagctac tacaaacagt
     8401 tgagtgaatt gcttgacgat caggcgcaaa cgccacaggt cggtgtagtc tgcgagacgt
     8461 tctcagcctg tatcagtctg gtggcaaaaa gcgattttct cagcatactg cctgaagaaa
     8521 tgggctgcga tcccttgcac ggacaggggc tggtgatgtt gccggttagc gaaattttac
     8581 cgaaagcggc ctattatttg attcagcggc gtgatagtcg ccagacacca ctgaccgcgt
     8641 cattaatcac gcaattccgg cgagaatgcg gctatcagca aagttaaaac tgcataaaaa
     8701 aatagagtct gtcgacatcc gccagactct atagtacaca cagcagtgca tccgcgtctt
     8761 aaatccggca ttgtctcctc tgcgccggtg actgtgttat aacggtttta acctttagtt
     8821 gccaattttc ttcagtgaca gatttcacga aaatataagt tacattatta atatcgtgaa
     8881 tgaataatca tgcataagca ttttgcttaa aatatcggca atatttggaa cttattactg
     8941 gaattttggg taatacgttg ttggaccgac ccggtctggt tatcatatcg cgctcttaat
     9001 tgcgggagga tgtaacatga accttgacga caaatcgctg tttcttgacg ccatggaaga
     9061 tgtccagccg ctgaaacgtg ctaccgatgt ccactggcat cccacgcgta accaacgtgc
     9121 gccacagcgt atcgacacgc tgcagcttga taatttcctc actaccggat ttctcgacat
     9181 tatcccacta agtcagccgc tggagtttcg gcgggaaggg ttgcaacatg gggtgctgga
     9241 taagctgcgc agtggtaaat atccgcaaca ggcgagcctg aatcttttgc gccagccggt
     9301 ggaagagtgc cgcaaaatgg tgttcagttt tattcaacaa gccctggcgg atggtttgcg
     9361 taacgtgctg attattcatg gtaaagggcg ggatgataaa tcgcatgcca atattgtccg
     9421 cagctatgtg gcgcgctggc tgaccgaatt tgatgatgtt caggcatatt gcaccgcgct
     9481 accgcatcat ggcggcagcg gggcgtgtta cgtcgcacta cgtaaaacgg cgcaggcgaa
     9541 gcaagaaaac tgggagcgcc acgctaagcg cagtcgttga tctcgagacg catccgcggc
     9601 ttatgccgcc agcacgcggt tgcgtccatc atttttcgcc cgatacaaag catcatcaac
     9661 gcgtttaaac agttcatcga tgctttcatt tccttcgtga tgcgccacac caatgctgac
     9721 ggtaaagcgt ggtaagcccg aaatactcac ttttgccacg cttacgcgga tagtttcagc
     9781 cagcgaaagc gcggtatcca gtggggttct tggtagcaat atgacaaact cttcgcctcc
     9841 ccaacgaaac accaaatcgc ctttgcgagc gcaactttcg agggtgcggg cgagggcgca
     9901 taacacctca tcacctttag aatgcccata gagatcgtta atgtgtttaa aacgatcggt
     9961 gtcgatgagc aacaagctgt aatcctgagc gatggcgaga tgctgcattt ggcctggttc
    10021 cgtaatgtga taaaactgtc gccgattcag taatccggtc atcgcgtcat ggtgagcagc
    10081 atgttccagc tgctcctcca gccgtttttg ctcagtaata tcatgcacaa tacataacat
    10141 gagcttgtcg ccataaattt caatcggtcc ggcataggtc tgcacatgac gagtagatcc
    10201 atccgccagt ttatgaataa aattcaaagg tttatgaccg ccgggtaaat gcgaaatttc
    10261 atgcatgata ggcatgacgc gacgcccgag catatttatt tcccaggtat gtttctggca
    10321 catcgtttca tggttataac catagaaatt gagcgcggcg aggttagcat cgacgatttg
    10381 tccatctcgt gacgggtcaa tcaacaacat tggtgcagag ttagtcagaa aaaagcgcgc
    10441 ataaaaacct tgttttttgc gctgataatt tgccgagcga ctggctttta aacccagcgt
    10501 tgccggcgct tcgatacctt cgaaaataat caccggttct gtttctgtca gctctcgcaa
    10561 aacaagccga cagcttaatg ctgtttcctc ttctttacgc tgaacagtga ggatttcgat
    10621 aatatcgtgt tggttttgca gatcggagag gtatttcggc agttcttttt gtgaggagac
    10681 ggaataggga ccggttcgta gctgactaaa cgtgaggtct tgcatcaaca gtcgcgccgc
    10741 gctattggca taaattaact gttcctcaaa gggcgaaacg atccagacag gactggtgag
    10801 taagtccagg gtattgaagt tgtgcgtaat cattgagatc ccattatttt tatcaatttt
    10861 tgttgctatc cgatcgcaaa aaagccacgt catatgatca gataattctg ataatgatag
    10921 acgctattta a
//
LOCUS       DAZUGK010000153        10920 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZUGK010000153 DAZUGK010000000
VERSION     DAZUGK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.154"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            296..1636
                     /gene="dcuB"
                     /locus_tag="ACHY5W_003500"
     CDS             296..1636
                     /gene="dcuB"
                     /locus_tag="ACHY5W_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFS4448334.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1714..3360
                     /gene="fumB"
                     /locus_tag="ACHY5W_003501"
     CDS             1714..3360
                     /gene="fumB"
                     /locus_tag="ACHY5W_003501"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS4448335.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3483..4112
                     /gene="yjdF"
                     /locus_tag="ACHY5W_003502"
     CDS             3483..4112
                     /gene="yjdF"
                     /locus_tag="ACHY5W_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFS4448336.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLKLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4251..5672)
                     /gene="melB"
                     /locus_tag="ACHY5W_003503"
     CDS             complement(4251..5672)
                     /gene="melB"
                     /locus_tag="ACHY5W_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFS4448337.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIDDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCSVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5775..7130)
                     /gene="melA"
                     /locus_tag="ACHY5W_003504"
     CDS             complement(5775..7130)
                     /gene="melA"
                     /locus_tag="ACHY5W_003504"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4448338.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHISLMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7413..8321
                     /gene="melR"
                     /locus_tag="ACHY5W_003505"
     CDS             7413..8321
                     /gene="melR"
                     /locus_tag="ACHY5W_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFS4448339.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8520..10787
                     /gene="adiA"
                     /locus_tag="ACHY5W_003506"
     CDS             8520..10787
                     /gene="adiA"
                     /locus_tag="ACHY5W_003506"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFS4448340.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2805 a         2893 c         2670 g         2552 t
ORIGIN      
        1 acaaaaaata gaccgataaa tatcaataag ataacagcaa acaaaacatt aacatctgcg
       61 cagtacaaac tttaaaccca tcgccagaga gtttttctct ctgaaaaagc cgcttatcac
      121 agtgcataaa tttgccgctg ctttaatcag ccaatattca ctgtgaggta tttgctaaag
      181 ccggtaacga ccaaacggat atttagtcag gctctgaaaa cagttcatac aaaacagaac
      241 gtgactgtga tctattcagc aaaaatttaa ataggattat cgcgagggtt cacacatgtt
      301 atttactatc caacttatca taatactgat atgtctgttt tatggtgcca gaaagggtgg
      361 tatcgcgctg ggtttattag gcggtatcgg tctggtcatt ctggtcttcg tcttccacct
      421 tcagccaggt aaaccaccag ttgatgtcat gctggttatc attgcggtgg tggcggcatc
      481 ggcgaccttg caagcttcgg gcggtcttga tgtcatgctg caaattgccg agaagctgct
      541 gcgccgcaac ccgaaatatg tctcaattgt cgcgccgttt gtgacctgta cgctgaccat
      601 tctttgcggt acgggtcatg tggtttacac cattctgccg atcatctacg acgtcgccat
      661 taagaacaac atccgtccgg aacgtccgat ggcggcaagt tctatcggtg cacagatggg
      721 gattatcgcc agtccggtgt cggttgcggt cgtgtctctg gttgcaatgc tgggtaatgt
      781 cacctttgat ggtcgccatc ttgagttcct cgacctgctg gcaatcacca ttccatcgac
      841 gttaatcggt atcctggcga tcggtatctt cagctggttc cgcggtaaag atctggataa
      901 agacgaagag ttccagaaat tcatctccgt accggaaaac cgtgagtatg tttacggtga
      961 taccgcgacg ctgctcgata aaaaactgcc gaaaagcaac tggctggcaa tgtggatttt
     1021 cctcggggca atcgctgtag tcgcccttct tggtgctgat tcggacctgc gtccatcctt
     1081 cggcggcaaa ccgctgtcga tggtactggt tattcagatg tttatgctgc tgaccggggc
     1141 gctgattatt atcctgacca aaaccaatcc cgcgtctatc tcaaaaaacg aagtcttccg
     1201 ttccggtatg atcgccatcg tggcggtgta cggtatcgca tggatggcag aaaccatgtt
     1261 cggtgcgcat atgtctgaaa ttcagggcgt actgggtgaa atggtgaaag agtatccgtg
     1321 ggcctatgcc attgttctgt tgctggtttc caagtttgta aactctcagg ctgcggcgct
     1381 ggcggcgatt gttccggtcg cgctggcgat cggcgttgat ccggcataca tcgtggcttc
     1441 agcaccggct tgctacggtt attacatcct gccgacttat ccgagcgatc tggcagcgat
     1501 tcagtttgac cgttccggca ccacccacat cggtcgcttc gtcatcaacc acagctttat
     1561 tctgccgggg ttgattggtg tgagcgtatc gtgcgtcttc ggctggatct tcgccgcgat
     1621 gtacgggttc ttataaatgc actttgcgtg ccgcccgtga ctacgcggca cgccattttc
     1681 gaataacaaa tacagagtta caggctggaa gctatgtcaa acaaaccctt tatctaccag
     1741 gcacctttcc cgatggggaa agacaatacc gaatactatc tactcacttc cgattacgtt
     1801 agcgttgccg acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc cctgaccctg
     1861 ctggcgcagc aagcctttca cgacgcttct tttatgctcc gtccggcaca ccagaaacag
     1921 gttgcggcta ttcttcacga tccagaagcc agcgaaaacg acaagtacgt ggcgctgcaa
     1981 ttcttaagaa actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca ggataccggc
     2041 accgcgatca tcgtcggtaa aaaaggccag cgcgtgtgga ccggtggcgg tgatgaagaa
     2101 gcgctgtcga aaggcgtcta taacacctat atcgaggata acctgcgcta ctcgcaaaac
     2161 gcggcgctgg acatgtacaa agaggtcaac accggcacta acctgcctgc acaaatcgac
     2221 ctgtacgcgg tagatggcga cgagtacaaa ttcctttgcg ttgcgaaagg cggcggctct
     2281 gccaacaaaa cctatctcta ccaggaaacc aaagccctgc tgactcccgg caaactgaaa
     2341 aacttcctcg tcgagaaaat gcgtaccctc ggtactgcag cctgcccgcc gtaccatatc
     2401 gcgtttgtga ttggcggtac gtctgcggaa accaacctga aaaccgttaa gttagcaagc
     2461 gctcactatt acgatgaact gccgacggaa gggaacgaac atggtcaggc gttccgcgat
     2521 gtccagctgg aacaggaact gctggaagag gcccagaaac tcggtcttgg cgcgcagttt
     2581 ggcggtaaat acttcgcgca cgacattcgc gttatccgtc tgccacgtca cggcgcatcc
     2641 tgcccggtag gcatgggcgt ctcctgctcc gctgaccgta acattaaagc gaaaatcaac
     2701 cgcgaaggta tctggatcga aaaactggaa cacaatccag gccagtacat tccacaagaa
     2761 ctgcgccagg ccggtgaagg cgaagcggtg aaggtcgacc ttaaccgccc gatgaaagag
     2821 atcctcgccc agctttcgca atatccggtt tccactcgtt tgtcgctcac cggcaccatt
     2881 atcgtgggcc gagatattgc acacgccaag ctgaaagagc tgattgacgc cggtaaagaa
     2941 cttccgcagt acatcaaaga tcacccgatc tactacgcgg gtccggcgaa aacccctgcc
     3001 ggttatccat caggttcact tggcccaacc accgcaggtc gtatggactc ctacgtggat
     3061 ctgctgcaat cccacggcgg cagcatgatc atgctggcga aaggcaaccg cagccagcag
     3121 gttaccgacg cgtgccataa acacggcggc ttttacctcg gtagcatcgg cggtccggcg
     3181 gcggtactgg cgcagcagag catcaagcat ctggaatgcg tcgcttatcc ggagctgggt
     3241 atggaagcta tctggaaaat cgaagtagaa gatttcccgg cgtttatcct ggtcgatgac
     3301 aaaggtaacg acttcttcca gcaaatcgtc aacaaacagt gcgcgaactg cactaagtaa
     3361 cctcttcggc ccagcgcctg gcagcatgct gccaggtgat ccccctgggc cacctctttt
     3421 gcgattgtaa tttcacgctt gctggtgaat agtcagtatt ttccctgact tgcgaactca
     3481 ccatgacccg cacactcaag ccgttaattc ttaacaccgg cgcactggcg ctaacgttaa
     3541 tcctcattta taccggcatt tcggcccatg acaaactcac ctggctgatg gaagtgacac
     3601 cggtgattat tgtcgtgccg ctactgcttg ccaccgctaa acgttatccg ttaacaccgc
     3661 tgctctatac gctcattttc tttcacgcca tcatcctgat ggtcggcgga caatacacct
     3721 acgcgaaagt ccccgttggt tttgaggtgc aggaatggct gaagttgagc cgtaatccgt
     3781 atgacaagct ggggcatttt ttccaggggc tggtgcctgc actggtggca cgtgagatcc
     3841 ttgtgcgcgg gatgtacgtt cgcggacgta aaatggtggc gtttctggtc tgttgcgtgg
     3901 cgctggcgat aagcgcgatg tatgaattaa tcgagtggtg ggcggcgctg gcaatgggcc
     3961 agggagcgga tgattttctc ggcacccagg gcgaccagtg ggacacgcaa tccgatatgt
     4021 tctgcgcgct acttggcgca ttaacgacgg tgatattcct cgctcgcttt cattgccgcc
     4081 agctacggcg ctttggcttg ataaccggat aacaacgtgc actttgttga tgccggatgc
     4141 ggcgtgaatt ccttatccgg tctacaaaat ttgcaaatcc aaagcattac attaagacgt
     4201 ggcctgataa acatatcgca tcaggccatt ttcgttagct attgtccacg tcaggctttc
     4261 acatcactca ctgcaccgac cggaatatca gcatgaacag gctcgggcgg tactttgcga
     4321 tatttatcca gcagatgaat ctggatcctg cgcagcgtgt cgccattgag gcgatagaaa
     4381 cggaagtaga gaatcagcgt taccatgaaa aacagagtcg gtagagcaat cataataaac
     4441 tgcataccta atagggcttg cgtagactgt tcaacattcg gtacatagcc aatcatccct
     4501 aacacaaccg caatgaaaaa agccgcaaag gctgaaccgc ccttcaccac catagtctgc
     4561 acggagtaag cgatgctttc acagcgtacg tgcagtttat attcaccgta atcaacggta
     4621 tctgccacca tgatgacctg taatacccag aaaagcgccg ttcccacatt cagcaaaata
     4681 cccgcaatca caatgaggac gacgttgtga tagctcatta atgccattaa caggagaaca
     4741 ctacagctta acaccggaag aatagatgct ccggcccata aaatgcgtcg ggataatgat
     4801 ttaactaagc gtgggaagaa tactaacgtc accaggttag cagctcccgc atacgacaga
     4861 taatagggga acaaatccgc atcatcgata acatatgaga aataatagat agcaaagccg
     4921 gtaataatgt tgctggctac attataagca agagccatac ccaagaggca tgaaagctga
     4981 tcgtttttat aaataagcgc aacgatggct ttaagtgtca gatggcttcc ttcagcagac
     5041 ggttgattgt ctgacgaaaa gacttcatgc acattgcgca gagtgatgat ggttgaaaca
     5101 ataaaaaagg cgatcagtac caatgtgaac atctgaaagc caaatccccg atcgccaccg
     5161 ccgacataat taacaaatgg tagcgtcaca cctgccgtaa caaagcccgc cagactggca
     5221 aaaaaacgcg gataaggaac cagttgttca cgctcacgtt tatcgagcgt gatggttgga
     5281 accagcgacc agaagggaat atccataatg gtgtaagtca tgccccagag gatgtaggtc
     5341 acgcaaacaa agacaatctg agtagtacct tcaaacagat gcgcactaaa gaggagaaat
     5401 aagattacag agtttgccaa cgtaccgatc aggatccagg gtttgaactt accccatcgc
     5461 gatcgcgtag catttacaat ccatcccata atcggatcgt taatagcatc ccagatcctc
     5521 gccaccagaa acaaagtacc gaccaaaccc acagacagcc cgacgacatc ggtgtagtaa
     5581 tacatgaggt acatatacac aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca
     5641 taactgagtt ttgtagtcat tgaaatgctc atagggtatc gggtcgctgt taccaacgcc
     5701 cgccttctgt tattggtatc gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag
     5761 cgtttagtcg cgttttaacg gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc
     5821 aggtcgtcaa caagagcata tatttcgtca atgcccagca cggcagcagt atgcgggtcc
     5881 atcatcgcgg cgtggtaaac acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc
     5941 gtctgtacgt tgatgttggt ttgcatcagg gcggccagat gcgaaggtag cgtaccgact
     6001 ttggtcggct gaatgccatt agcatcaacc agacaggcta cttccacgca acatccttgt
     6061 ggcaggttat caatcaaacc atcgttacgg acgttgccgt aaatcacact cggctcgcca
     6121 gtccagatag cgttcatgat tgtgctggca tattcccgtg acggtttaat atcaatccgg
     6181 gaggcgttct tatactcctc cagctcttta tgccagttcg ccagctgctc gacgcagcgt
     6241 ttcgggtact catccaacgg tactttataa cgctcaatca aatcctcacg acctggctta
     6301 ataaaccacg gtgtgtactc agcaaaatgt tctgacgatt ccgtgacgaa gtagcccagc
     6361 tttttgaaca tttcatagcg cacaatattc tggcagcgag tattgccgtg aatattcggc
     6421 ttcggtgcct gccctgcgtc ataagccgcc agcagttccg ggtagagatt cacgtaactg
     6481 ccgtcggcgg ttttgcgctc cagctccagg taaaacgcca tatggttgat acctgcgcaa
     6541 cggtaacgca gcgtagccgg gtcgatattg aggtcacgcg ccagctcttc cgccgttccc
     6601 tgcaccgaat ggcacagccc gacctgtttg atatgcggat agcgggcata catcgcccag
     6661 gtattcatcg ccattgggtt aacatagttg agcatggtgg catcggggca gacttccgtc
     6721 atgtcctcgc aaatttgcca cagatgcgga atggtacgta gcgcgcgcat aataccgccc
     6781 ggccccaacg tatcggcaat ggtttgttcc agaccatgcc gcttacagac ctcgaaatca
     6841 gtcaccgtgc aaggttcata accgccaatc tgaaatgcca ccacgacaaa atcggcatcc
     6901 tgtaaggctt ctttctgttg ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc
     6961 atcagcttac gcaccacaat atgcgactct tccaggcggg tggggtcaat gtccatcagg
     7021 gaaatatgcg ccgttttcag cgcctcgcga tggaacacat caccaagaat atttttaacg
     7081 aaaatcgtcg aaccagcgcc gataaatgta attttgggtg cagacatcat gcagatctcc
     7141 tggcttgctt gaataacttc atcatggcag gcgaatctgc agaaaacctc cggcttccag
     7201 gttgagcatt cccataaact cagatcctgc gtgaagcagc agtaaatctg agtttatggg
     7261 aataatttac ctgaaaagca gagttttcgg cctcatgatg ttcggacagt cccctcgcct
     7321 ctctgagaag agggtgaaaa ccgtgctccc actcgcagtc atcctccctc actcctgcca
     7381 taattctgat attccaggaa agagagccat ccatgaatac agatacgttt atgtgcagca
     7441 gcgacgaaaa acagacccgc agcccgctgt cgctgtattc ggaataccag cggatggaaa
     7501 ttgagtttcg cgcaccgcat atcatgccca ccagccactg gcatggtcag gtcgaagtga
     7561 atgtgccttt cgatggcgat gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc
     7621 agggtcatat cacgctgttc tgggcctgta caccgcacca actaacagat accggaacct
     7681 gtcagagcat ggcgattttt aatctgccga tgcatctgtt tctctcctgg ccgctggata
     7741 aggacctgat taaccacgtc actcacggca tggtgatcaa atcactggct acacagcaac
     7801 ttagcccgtt tgaagtgcgc cgctggcagc aggaattgaa cagtccgaat gagcaaattc
     7861 gccagctcgc cattgatgaa attggcctga tgctcaagcg atttagcctc tctggctggg
     7921 aaccgattct ggtcaataaa acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc
     7981 aattttatgt tagccagatg ctgggcttta ttgccgaaaa ctatgaccag gcgctgacca
     8041 tcaacgatgt ggctgagcac gtcaaactta acgccaacta tgcaatgggg atattccagc
     8101 gggtcatgca attgacgatg aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg
     8161 cgttactgag cgataccgat aaaagtattc tcgatattgc cctgacggca ggctttcgtt
     8221 caagtagtcg tttttacagc acgttcggca aatatgtcgg catgtcgccg caacaatacc
     8281 gcaaacttag ccaacagcgc cgccagacgt ttcccggcta aagacgatat cagtatcagc
     8341 caaaaaaata gtttagccgt cgataataag cagtgaaaat aatttaaaat aatcacataa
     8401 cacattgttt ataaaaacaa atatttcaaa aatgtttgtt tttcacgcgc tttacagccc
     8461 gaaaaggccg gaagatactt gcccgcaacg aagattcctt cataaccggg taagcaatga
     8521 tgaaagtatt aattgttgaa agcgagtttc tccatcaaga cacctgggtc ggtaacgccg
     8581 ttgagcgtct ggcagatgct ttaagccagc aaaatgttac cgtgattaaa tccacctcct
     8641 ttgatgatgg ttttgccatt ctctcttcaa acgaagccat tgactgcctg atgttcagct
     8701 atcaaatgga acatccggac gaacatcaaa acgtcagaca attgatcggt aagcttcatg
     8761 agcgccaaca aaacgtgccg gtcttcctgt tgggtgatcg ggaaaaagcc ctcgccgcaa
     8821 tggaccggga tctgctggag cttgtcgatg aattcgcctg gattctggaa gataccgccg
     8881 actttatcgc cggacgcgct gttgccgcga tgacccgcta ccgccagcag ctgttgccgc
     8941 cactgttcag cgcgctgatg aaatatagtg acatccatga atattcctgg gcagcgccag
     9001 gccaccaggg cggcgttggt tttaccaaaa cacccgccgg acgtttctac catgactact
     9061 atggtgaaaa tctgttccgc accgacatgg gcatcgaacg aacttccctc ggttctttgc
     9121 ttgaccatac tggcgcattt ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg
     9181 atcgctcctg gtcggtagtc gtcggtactt ccggctctaa ccgcaccatc atgcaggctt
     9241 gcatgaccga taacgatgtc gtggtcgttg accgtaactg ccataaatcc atcgaacaag
     9301 gtttgatgct gacaggcgcg aaaccggtct atatggtgcc aagccgcaac cgctacggca
     9361 ttatcgggcc aatctatccg caggaaatgc aacctgaaac cttgcagaag aaaatcagtg
     9421 aaagcccgct gaccaaagac aaagccggac aaaaaccgtc ttactgcgtg gtgaccaact
     9481 gcacctatga cggcgtgtgt tataacgcta aagaagcgca ggatctgctg gaaaaaacct
     9541 ccgatcgtct gcactttgac gaagcctggt acggctatgc acgtttcaac ccgatctatg
     9601 ccgatcacta tgccatgcgc ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca
     9661 cccactccac ccacaaactg ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg
     9721 aaggtcgtgg ggcgattaac ttctcccgct tcaaccaggc ctacatgatg catgccacca
     9781 cctccccgct gtatgccatc tgcgcatcca acgacgtggc ggtgtcgatg atggacggca
     9841 acagcggcct gtcactgacg caggaagtga tcgacgaagc ggttgatttc cgtcaggcga
     9901 tggcgcggct gtataaagag ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca
     9961 aagaagtcgt caccgaccca caaaccggca aaacctatga ctttgctgac gcaccaacca
    10021 aactgctgac caccgttcag gactgctggg taatgcatcc gggcgaaagc tggcacggct
    10081 tcaaagatat tccggataac tggagtatgc tcgacccgat taaagtcagc atccttgctc
    10141 cgggaatggg tgaagatggt gaactggaag aaaccggtgt tccggcggcg ctggtcactg
    10201 cctggcttgg tcgccacggc attgtgccta cccgcaccac tgacttccaa attatgttcc
    10261 tgttctctat gggcgtaacc cgtgggaaat gggggactct ggttaacacc ctttgctcct
    10321 tcaaacgcca ctatgacgcc aacacaccgc tggcgcaggt gatgccggaa cttgttgaac
    10381 aatatcctga cacttacgcg aacatgggga ttcacgatct gggtgacacc atgtttgcct
    10441 ggctgaaaga aaacaatcct ggcgcacggt tgaacgaagc ctattccggc ctaccggtgg
    10501 cggaaataac cccgcgtgaa gcgtacaacg cgattgtcga caacaatgtc gaactggtat
    10561 ccattgaaaa tctaccagga cgcatcgcgg caaactcagt tatcccgtat ccgccaggaa
    10621 tcccgatgct gctgtctggt gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt
    10681 tacgctcgct gcaatcctgg gaccatcatt tccctggatt tgaacacgaa actgaaggga
    10741 ctgaaattat tgacggtatt taccacgtta tgtgtgtgaa agcgtaacca ctattccgct
    10801 gaaggcgtaa ttgtttaaat aacattacgc cgcctggcct taggcctttt gagtatggca
    10861 acgttttcat aaaaattgct gcaaacaaaa atgtcatact ttttgcgcgg ccccaccccg
//
LOCUS       DAZUGK010000154        10883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZUGK010000154 DAZUGK010000000
VERSION     DAZUGK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.155"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..331)
                     /gene="chbB"
                     /locus_tag="ACHY5W_003507"
     CDS             complement(11..331)
                     /gene="chbB"
                     /locus_tag="ACHY5W_003507"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFS4448341.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLSEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(631..969)
                     /gene="osmE"
                     /locus_tag="ACHY5W_003508"
     CDS             complement(631..969)
                     /gene="osmE"
                     /locus_tag="ACHY5W_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFS4448342.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1171..1998
                     /gene="nadE"
                     /locus_tag="ACHY5W_003509"
     CDS             1171..1998
                     /gene="nadE"
                     /locus_tag="ACHY5W_003509"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4448343.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2228..3115
                     /gene="cho"
                     /locus_tag="ACHY5W_003510"
     CDS             2228..3115
                     /gene="cho"
                     /locus_tag="ACHY5W_003510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFS4448344.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3075..3650)
                     /gene="ves"
                     /locus_tag="ACHY5W_003511"
     CDS             complement(3075..3650)
                     /gene="ves"
                     /locus_tag="ACHY5W_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFS4448345.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3853..4338)
                     /gene="spy"
                     /locus_tag="ACHY5W_003512"
     CDS             complement(3853..4338)
                     /gene="spy"
                     /locus_tag="ACHY5W_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFS4448346.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4668..5636)
                     /gene="astE"
                     /locus_tag="ACHY5W_003513"
     CDS             complement(4668..5636)
                     /gene="astE"
                     /locus_tag="ACHY5W_003513"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFS4448347.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLELHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5629..6972)
                     /gene="astB"
                     /locus_tag="ACHY5W_003514"
     CDS             complement(5629..6972)
                     /gene="astB"
                     /locus_tag="ACHY5W_003514"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFS4448348.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFA
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQIFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMLLVLPQECREHAGVWRY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6969..8447)
                     /gene="astD"
                     /locus_tag="ACHY5W_003515"
     CDS             complement(6969..8447)
                     /gene="astD"
                     /locus_tag="ACHY5W_003515"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFS4448349.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAVFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8444..9478)
                     /gene="astA"
                     /locus_tag="ACHY5W_003516"
     CDS             complement(8444..9478)
                     /gene="astA"
                     /locus_tag="ACHY5W_003516"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFS4448350.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVCAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9475..10695)
                     /gene="astC"
                     /locus_tag="ACHY5W_003517"
     CDS             complement(9475..10695)
                     /gene="astC"
                     /locus_tag="ACHY5W_003517"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFS4448351.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFPHDCYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRYAAYNDINSASALIDDSTCAVIVEPILGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
BASE COUNT         2632 a         2982 c         2695 g         2574 t
ORIGIN      
        1 ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc
       61 ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta
      121 acaaacgctg gatttcggac aacatataag caatctgcgg ccctaataac acgacatcgg
      181 cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt
      241 catatttttc tgcctgtgcg cgcatttttg ataccagtaa agaggtagac atgcccgcag
      301 aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc
      361 agccagacac tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac
      421 atgacttttt ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt
      481 gaccttataa attgtctctc cttgacctgc ggttatgacc ctcttcacat ttcgggcaaa
      541 taattcgcga agataattaa gaactcaggc caaaaaaaaa ccggcgcaat ggccggtttc
      601 cgttgttact caagctttca gacgaagtga ttacttcgca gcctgtggat cagtgtcgta
      661 ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa
      721 gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag taccacgagc
      781 atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct
      841 catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata
      901 agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt
      961 cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg
     1021 cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt
     1081 taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga
     1141 agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg
     1201 ctgggcgcaa aaccgcagat taatgccgaa gaggaaattc gtcgtagtgt cgattttctg
     1261 aaaagctacc tgcgaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt
     1321 caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa
     1381 accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac
     1441 gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat
     1501 atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc
     1561 gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg
     1621 ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc
     1681 ttcactaaat atggtgacgg cggtacggac attaatccgc tgtatcgtct caacaaacgt
     1741 cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa gaaagcgcca
     1801 acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc
     1861 tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca
     1921 atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat
     1981 gatttctgga aaaagtaata atttgcacat attggattgt gcgaaaaaga gtaatttgtt
     2041 cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat
     2101 tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg
     2161 acccgcctct tttttaatcg cttcccgcct gttacactgg atagataacc agcattcgga
     2221 gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta
     2281 tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac caggggtgta
     2341 tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg
     2401 cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg
     2461 gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt
     2521 aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc
     2581 cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg
     2641 agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag
     2701 catcgctgat gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagccgcgg
     2761 gcgtgcttgt tttcgttctg cattaaaacg ttgcgctggg gcgtgttgcg gtaaagagag
     2821 ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg
     2881 gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat
     2941 tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat
     3001 tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc
     3061 cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca
     3121 ctgaacaaca gtttaccctg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag
     3181 cacgcgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca
     3241 aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc
     3301 gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc
     3361 agtttcgctt ttaccacctg gtccgccgca aaggcaaaag gttgtaacgg ttttaaagta
     3421 tggttaaagc ggtctgtgct ttcaagaaac atctcgccgc cttccagcaa cgtcactatc
     3481 ctttccatac cgggaaacaa agaaaactcc ccattagccg cgatggatgc aatgctggca
     3541 cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca
     3601 gcgtttcgcc acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc
     3661 ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt
     3721 gatttacgtt agtggtgatc aggctttttt tgcatctctt tgagaacaaa aaagaaaacc
     3781 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
     3841 ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt
     3901 cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta
     3961 aattttgttc tgggtttcca tgtgtgccag catgttagct ttgcgctgtt cttccatttt
     4021 tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat
     4081 tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc
     4141 gcggatctgc tgtttttgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg
     4201 cggaccgaac ttgcctttat ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt
     4261 ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag
     4321 tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta
     4381 agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca
     4441 acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa
     4501 atttgatgga gagtgatgag cgaatattac aacacgatga ttttgcagag attatgaaga
     4561 actataccgg atgactggtg ataaataaag caaataacca ggattaatct gtattaattt
     4621 ataagaaagt aacttaatac ccgcagaacg atttctgcgg gtaagtatta gcttattttt
     4681 tcgagcatta atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac
     4741 tctacatcat gggttacggt aaaacgttcc tcaccgtcct gcgccagcaa cgttcctttc
     4801 ttaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg
     4861 cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc
     4921 tcaccagata gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt
     4981 tgcccaaagg gcaacgcttt gccaagttcc agcgtacagg ccagcgcgcc aaaatgtctg
     5041 gcgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc
     5101 gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca
     5161 ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gttcaaggtg ccagcgcaca
     5221 gattctttgc cctggtcata aaaatcttcc aggcattgtt ccagttcgcg cgcccgacag
     5281 gtttccccgc tttcagcaaa cagctgccaa cgaccgccaa acattcggtt catatcgcta
     5341 tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc
     5401 caacgtaacg ggatctcgcc gtgagatatc gcgccaagca acgcgtccag catctccaca
     5461 ggtgccgtct cattaccgtg tattcccgct gaaatcacca gtgcgccttg cggtggcgtt
     5521 aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacacc gttgatttct
     5581 cgctcggtga tgacaggttt tttactcgtt aaggtcagag caagaaaatt atccattgcc
     5641 ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc tcaatacatc
     5701 cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag cagtaagacg
     5761 atcgcgatag taacgatcca cccagtcatt gagcgcatta aacagcgtat cgttcatcat
     5821 caccgccgga ttcaccgccc ggcgttcttc ttctgtcaac accacgcgca accgcaggca
     5881 cgccgggccg ccgccgtttg ccatgctttc acgtaaatca aagactttca gttcgctaat
     5941 cgggttgtca gcggcaagga gttcattgag ataacgccag actccagcgt gttcccgaca
     6001 ctcctgcggc agcaccagca gcatggaacc gtcatcgcgg ctaagtagct ggctattaaa
     6061 cagatacgta gacaccgcat cagacacgga gacctgagtc gccggaactt ctatcgccat
     6121 aaaaccattg acccgcgcac gcaggtttgc cagtaactgc gactggcgag cgaacgcctg
     6181 ttgatggcaa aacagcacct ggcggttact cacggcaatc acatcattat gaaaaacgcc
     6241 ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgctgtggat tcacctgatt
     6301 cagccttgcg accgcctcgc tggcttcacg agtctgccgc gccggatagc gggaaggtcg
     6361 ggtatcattg ccctcctctc gcccgtaaac aaaaatctgc atacccggtt caccgtaatg
     6421 accgccgaga cggttgtggt ttgccgcccc ctcatcaccg agcaaagcca cctgcggcaa
     6481 cgccgaatgg acggcgaatt tctcttcatc gttaaaaatc gcctttaaca gcgattcggt
     6541 gacgggcgct tccagcgagc ggtgaaattt attgttcagg ttggcaacgg tgagatgcac
     6601 tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg
     6661 cgaagcggag ctgacgctgg aaagccagtg cggtgcctgg cgtgcaactt tttccagtac
     6721 ctgctcatcg ctaccactga atcccaactg acgcaacacc ggaataaacg gacgctcgtg
     6781 cggcgggatc acggcctggg ggaatcccgc atcggcaagg gctttcattt tcagtaagcc
     6841 ctgttttgcc gccagtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt
     6901 accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc
     6961 ccaggcgttc atcgcaccac ctcatcggaa aaatccagtc cggggttaag cgttgcgggc
     7021 aaagttaacg agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac
     7081 caggcgctgg ggcgatggtt accggatgca ccaatgccgc cgaatggcgc ggtactggca
     7141 gcgccggtaa gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttgt
     7201 tcgaattttt cccgctcggg ggaaaccagc ccacaagaga ggccaaagcg agtgttatta
     7261 gccatcctga tcgcctcatc gaaggtgtcg taacgccaga cgcgcaatag cggcccgaac
     7321 acttcttcat ccggcacgcc tgcaacaccc gtcatttcaa taatccccgg cgtcaacagc
     7381 gatgttcccg cttgcagcaa acgcggcgca agcagggttc gtccgcccat cgcttccagt
     7441 tgctgccatg cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac
     7501 ggctgcggtt cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacga
     7561 gcaagaaacg catcgccctg cgccccgctt ttcagcaata aacggcgggc gcaggtgcag
     7621 cgttgaccgg ctgtgacaaa cgccgactga atggtcagat ggacagcggc gtcgatatcc
     7681 gccacctcat cgataattag cggattatta ccgcccatct caagggcgag aattttctcc
     7741 ggctgaccgg agagctggcg atgcagctgg tagccagtat tggcgctacc ggtaaacagc
     7801 aaaccgtcga gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc
     7861 aggttcagca cgcctggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca
     7921 ctccacggtg tcagttcgct gggtttaaag ataatagtgt tacctgccag caatgccgga
     7981 acgatatgtc cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg
     8041 ccgtgcgggc gatgtcgcag gctcgccgcg ccgtccggca tttcactacg ctgctcgccg
     8101 gtacgaacgt gatacgcctt aattgaaatc gcgattttat tgatcatcgc cgtcacttcg
     8161 gttgccgctt cccagcgcgg cttgccggtt tctcgggcaa tgattgcggt taattcggct
     8221 ttattgcttt ccagcaatcc ggcaaagcgt tcgacaactg cctgacgttc agcaaatgag
     8281 agccgcgccc agcgcggaaa caccgcacgg gctgcgcgac aagcctgccc gacctgagcg
     8341 gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc
     8401 gatgcaccct ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcct
     8461 ctgcgcacag gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt
     8521 gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt acgcaccagc accacgcgga
     8581 aatggtgata attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc
     8641 cttctgccac ttccaccagc cgacttttac ggatggcgca cacgcggtcg atgtcacact
     8701 caagcgtcgg cccaccgtcg aagatgtcga tataattacg gtagcgaaaa ccttctttct
     8761 ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggcct
     8821 cctgggataa aaagtgcgta tagatcggat gtttcggcat cagttctgca ataaatgcct
     8881 tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac
     8941 cgaggctttg ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa
     9001 ccactttgtc attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca
     9061 gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc
     9121 tgctgccggt gtgatcgtta ctgagaaaca gcgttggcag cgcgttatag acattcagct
     9181 ctttcgaagc gtgaaccagc gtgccgacgc gatagttgta ccagggatcg ttcagcccaa
     9241 ctgccacctc aatggcacaa atccccgcca cggtgcctgt ctcgctatct tccagcacga
     9301 acacatagcc ctgctcactt ttgggcagtt cgccttgcca ggttttgatt gccctttcga
     9361 tacgcgccga aagcgtggct tcattggcgg gaagcgacgt caggccgccg cccgttttgc
     9421 tggcaagctg catcagcgcc gagacatctg agcgctcaac aggacggatg accatcatga
     9481 tgaacctcgg ctaacaaagt gttcgcaagc aactgcaaag cgatccagtc cggtcgtcac
     9541 ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag
     9601 taccatcacg cctgctttcg ccgcttcctg agagatctgt ttcgcttgcc cggcgtaatc
     9661 ggcattcagt acacagccaa tcagcaaacc taagccgcga acttcactga acaaaccgta
     9721 gcggtgatta atggtattaa gacgctcaac aaaccagtcg tgacgctgtt taacgccatt
     9781 aagcatctct ggcgtgttga tgagatccag cactttgcct gccaccgccg aggccagcgg
     9841 attgccgcca taggtggtgc catgagtgcc aacggtcatc acgctggcgc actcttcggt
     9901 tgctaacaac gcaccgaccg ggaaaccgcc gcccagcgct ttggcggtag ttaacagatc
     9961 aggcgtcacg ccgtagtgca tataggcata cagttccccg gtgcgcccga cgccggtttg
    10021 tacttcatca aaaatcaaca gcgcattgtg gcgatcacac aattcacgca gaccttgtaa
    10081 aaatgcgttg ctggctggca ccacaccgcc ttcccccagg atgggttcga caatcaccgc
    10141 acaggtagag tcgtcaatca gcgcgctggc agagttaata tcgttatatg cagcataacg
    10201 gatatctggt ggcagtggcg caaaatcctg tgaataggct ggctgcccac ccgcactaac
    10261 agtaaacagc gtgcgaccat gaaacgcatt tttaaacgcc acgataccgc tcttatggct
    10321 gccgtagcag tcgtgaggga atttacgcgc cagttttagc gccgcttcgt tggcttccgc
    10381 accggagtta caaaagaaaa cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag
    10441 tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc
    10501 gttcagcgcc tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg caatgccacc
    10561 cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt cgccacgtac
    10621 cggtataaac ggtgccggag cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt
    10681 aattggctga gacatagcga cctctacagt aaataattcg ttatttatat gttaataata
    10741 agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg ataaaagtgc
    10801 ataaaccgcg gaggctaact ggaaatcaag gagttataac caaaccatat gcatttaaag
    10861 tgcatattaa gtgaatacgt ttg
//
LOCUS       DAZUGK010000155        10865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZUGK010000155 DAZUGK010000000
VERSION     DAZUGK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.156"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1122
                     /gene="yghO"
                     /locus_tag="ACHY5W_003518"
                     /pseudo
     CDS             1..1122
                     /gene="yghO"
                     /locus_tag="ACHY5W_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1129..1893)
                     /gene="glcC"
                     /locus_tag="ACHY5W_003519"
     CDS             complement(1129..1893)
                     /gene="glcC"
                     /locus_tag="ACHY5W_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFS4448352.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNQS"
     gene            2144..3643
                     /gene="glcD"
                     /locus_tag="ACHY5W_003520"
     CDS             2144..3643
                     /gene="glcD"
                     /locus_tag="ACHY5W_003520"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFS4448353.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3643..4695
                     /gene="glcE"
                     /locus_tag="ACHY5W_003521"
     CDS             3643..4695
                     /gene="glcE"
                     /locus_tag="ACHY5W_003521"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFS4448354.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPSRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMSEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTSWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4706..5927
                     /gene="glcF"
                     /locus_tag="ACHY5W_003522"
                     /pseudo
     CDS             4706..5927
                     /gene="glcF"
                     /locus_tag="ACHY5W_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            5932..6336
                     /locus_tag="ACHY5W_003523"
     CDS             5932..6336
                     /locus_tag="ACHY5W_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFS4448355.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6358..8529
                     /gene="glcB"
                     /locus_tag="ACHY5W_003524"
     CDS             6358..8529
                     /gene="glcB"
                     /locus_tag="ACHY5W_003524"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFS4448356.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVY
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSSLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8885..10567
                     /gene="glcA"
                     /locus_tag="ACHY5W_003525"
     CDS             8885..10567
                     /gene="glcA"
                     /locus_tag="ACHY5W_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFS4448357.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSLLIAIFVFKMPIDMAFSAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGVFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2530 a         2735 c         3051 g         2549 t
ORIGIN      
        1 atcaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc ttttccgtca
       61 tcactttatc ccgatgatcc aaactggata ccccctttat tcattgaacg cagcgagcat
      121 ttgtctgcga agaatccggg gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa
      181 gcggggcaga tagttgggcg tattaccgcg caaatagata ccttacaccg cgagtgttac
      241 ggcgaagata ccggtcattt cggcatgatt gacgccattg atgatccgca ggtttttgcc
      301 gcgttgtttg gcgcagcgga agcgtgattg aagtcacaag gtgcaagtaa gatcagcggg
      361 cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt tgacacacca
      421 ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga acaactgggt
      481 tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct cactttctct
      541 ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg tgaccattcg ctgcatcaat
      601 cgccagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaactc tggctggcag
      661 cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg cgatcaactt
      721 aaatatctgg tgccggacga tatgatctat atcgctgaga ttgattctgc accctgcgcg
      781 tttattgtcg gcttgccgaa catcaacgag gcgattgccg atctgaacgg atcgctcttt
      841 cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca gcggtgtgcg aactgcgcga
      901 gtaccgctga tgggcgtgcg ggacgagtat cagttcagcc gcatcggccc ggtgattgcg
      961 ctgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga tgcgctggag
     1021 atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg cattggggcg
     1081 gagccgtaca agcgttaccg attgtatgaa aagcaaattt aataccagct aactctggtt
     1141 catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga tatcgtggag
     1201 attctttttc acggtccgca catgatcgcg tgctgcgcgc tgggcgacgt gcggcagccg
     1261 ctgcaacacc gcgttgtaga tccgcgcatg ctggcgatcg atctgctttt tttgctgtgg
     1321 tcgatggtag agattattta cgctggcaaa cactgaatta aacatcagat cggtcaatga
     1381 ttgcagcgta aacaccagca cctgattgtg agaagcctga caaatggcga gatggaaagc
     1441 gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac tggcggcgag
     1501 cattttttca taacagcggg ttatcacaac aaaatcagcc tgcgttccca gcgttgccgc
     1561 aagccttgcc gattcgccct ccagtaatgc gcgaacatcg agcagatcgt acagcgttcg
     1621 cggttgcgta ctgaacagat ggatcagcgg actggtgtcc tgcacccgat taagccgtgc
     1681 gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca gcacggtcag
     1741 cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac gttccgaggg
     1801 aagcggctga ccgaccttca gtacgccgtc gataattaac cgttcgatac tctctgcaac
     1861 cacttcgcaa atagggcgac gttcatcttt cattcccaga cctcgtgcac aggtaggacc
     1921 aatttttctg ttcttaactc gcaaaacacg cacatcacgc aagtgattat gttacatcaa
     1981 tttaacatta agttaaccaa gacaaggtca cagagctgga aaaaaaatgg tctgaccggt
     2041 agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag aagcagcgcg
     2101 caaaaatcag ctgccacaca acataacaaa gcgaagccta ctcatgagca tcttgtacga
     2161 agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga tggcactgcg
     2221 tgagcatgtc cctggactgg agatcctgca taccgatgag gagatcattc cttacgagtg
     2281 tgacgggttg agcgcgtatc gtacgcgtcc attactggtt gttctgccta agcagatgga
     2341 acaggtgaca gcgattctgg ctgtctgcca tcgcctgcgt gtaccggtgg tgacccgtgg
     2401 tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt tgttggtgat
     2461 ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgcgcgc gcgtgcagcc
     2521 aggcgtgcgt aacctggcga tctcccaggc cgttgcaccg cataatctct actacgcacc
     2581 ggacccttcc tcacaaatcg cctgttccat tggcggcaac gtggcagaaa atgccggcgg
     2641 cgtccactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg aagtgcaaac
     2701 gctggatggc gaggcactga cgctgggatc ggatgcgctg gattcgcctg gttttgacct
     2761 gctggcgttg ttcaccggat cggaaggtat gctcggcgtg accaccgaag tgacggtaaa
     2821 actgctgccg aagccgcccg tggcgcgggt tctgttagca agctttgact cggtagaaaa
     2881 agccggactt gcagttggtg acatcatcgc caatggcatt atccccggcg ggctggagat
     2941 gatggataac ctgtcgatcc gcgcggcgga agattttatt catgccggtt atcccgtcga
     3001 cgccgaagcg attttgttat gcgagctgga cggcgtggag tctgacgtac aggaagactg
     3061 cgagcgggtt aacgacatct tgttgaaagc gggcgcgact gacgtccgtc tggcacagga
     3121 cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttcccgg cggtaggacg
     3181 tatctccccg gattactact gcatggatgg caccatcccg cgtcgcgccc tgcctggcgt
     3241 actggaaggc attgcccgtt tatcgcagca atatgattta cgtgttgcca acgtgtttca
     3301 tgccggagat ggcaacatgc acccgttaat ccttttcgat gccaacgaac ccggtgaatt
     3361 tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag ttggcggcag
     3421 catcagtggg gaacatggca tcgggcgaga aaaaatcaat caaatgtgcg cccagttcaa
     3481 cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg atggtttgct
     3541 gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg ccatgcatgt
     3601 gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg cgagtgtgat
     3661 tacagtcagg cgctgctgga gcaggtgaat caggcaatta gcgataaaac gccgctggtg
     3721 attcagggca gcaatagcaa agccttttta ggtcgcccag tcaccgggca aacgctggat
     3781 gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat aaccgctcgt
     3841 gccggaacgc cgctggtggc gattgaagcg gcgctggaaa gcgcggggca aatgcttccc
     3901 tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc ctgcgggctg
     3961 gcggggccga gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg cacgcgcatc
     4021 attaccggcg ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa cgttgctgga
     4081 tacgatctct cacggttaat ggccggaagc tacggttgtc ttggcgtgct cactgaaatc
     4141 tcgatgaaag tgttaccgcg accacgcgcc accctgagcc tgcgtcggga aatcagcctg
     4201 caagaagcca tgagtgaaat cgcccagtgg caacttcagc cgctacccat tagtggctta
     4261 tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc ggtaaaagca
     4321 gcgcgtgaac tgctgggtgg cgaagaggtt gcgggtcagt tctggcagca attgcgtgaa
     4381 caacaactgc cgttcttctc gttaccaggt acctcatggc gcatttcatt acccagcgat
     4441 gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc gttacgctgg
     4501 ctgaaatcga cagccgatga caatcaaatc catcgcatcg cccgcaacgc tggcggtcat
     4561 gcgacccgct ttagtgccgg agatggtggc tttgccccgc tatcggctcc tttattccgc
     4621 tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaaccc cggtcgcatg
     4681 tacgcggaac tttgaggagc aggctatgca aacccaatta actgaagaga tgcggcagaa
     4741 cgcgcgcgct ggaagccgac agcatcctgc gcgcctgtgt tcactgcgga ttttgtaccg
     4801 caacctgccc aacctatcag cttctgggcg atgaactgga cgggccgcgc gggcgcatct
     4861 atctgattaa acaggtgctg gaaggcaacg aagtcacgct taaaacacag gagcatctcg
     4921 atcgctgcct cacttgccgc aattgtgaaa ccacctgtcc ttctggtgtg cgctatcaca
     4981 atttgctgga tatcgggcgt gatattgtcg agcagaaagt gaaacgccca ctgccggagc
     5041 gaatgctgcg cgaaggattg cgccaggtag tgccgcgtcc ggcggtcttc cgtgcgctga
     5101 cgcaggtagg gctggtgctg cgaccgtttt taccggagca ggtcagagca aaactgcctg
     5161 ctgaaacggt gaaagctaaa ccgcgtccgc cgctgcgcca taagcgtcgg gttttaatgc
     5221 tggaaggctg cgcccagcct acgctttcgc ccaacaccaa cgcggcaact gcgcgagtgc
     5281 tggatcgtct ggggatcagc gtcatgccag ctaacgaagc aggctgttgt ggcgcggtgg
     5341 actatcatct taatgcgcag gagaaagggc tggcgcgggc gcgcaataat attgatgcct
     5401 ggtggcccgc gattgaagca ggtgccgagg caattttgca aaccgccagc ggctgcggtg
     5461 cgtttgtcaa agagtacggg cagatgctga aaaacgatgc gttatatgcc gataaagcgc
     5521 gtcaggtcag tgaactggcg gttgatttag tcgagcttct gcgcgaggaa ccgctggaaa
     5581 aactggcaat tcgcggcgat aaaaaactgg ccttccactg cccgtgtacc ctacaacatg
     5641 cgcaaaagct gaacggcgaa gtggaaaaag tgttgcttcg tcttggattt accttaacgg
     5701 acgttcccga cagccatctg tgctgcggtt cagcgggaac atatgcgtta acgcatcccg
     5761 atctggcacg ccagctgcgg gataacaaaa tgaatgcgct ggaaagcggc aaaccggaaa
     5821 tgatcgtcac cgccaacatt ggttgccaga cgcatctggc gagcgccggt cgtacctctg
     5881 tgcgtcactg gattgaaatt gtagaacaag cccttgaaaa ggaataacaa aatgaaaact
     5941 aaagtcattc ttagccagca aatggcgagt gcaattattg ccgcaggtca ggaagaggcg
     6001 cagaaaaaca actggtctgt ttccattgct gttgccgatg acggcggtca tctgctggcg
     6061 ttaagtcgca tggacgattg cgcgccgatt gcggcttaca tctcccagga gaaagcgcgt
     6121 accgccgcgc tggggcgtcg tgaaactaag ggctatgaag agatggtgaa caacggacgt
     6181 accgcgttcg tgactgcgcc gttattaacg tcgctggaag gcggcgtacc ggttgttgtt
     6241 gatgggcaaa ttattggtgc cgtgggcgtt tctggtttaa ccggagcaca ggatgcgcag
     6301 gtcgcgaaag cggcagcagc ggtgttggcg aaataagcga aaacgaggag agaaacaatg
     6361 agtcaaacca taacccaggg ccgtttacgc attgacgcca attttaaacg ttttgtggat
     6421 gaagaagttt taccgggaac agggctggac gctgcggcgt tctggcgcaa ttttgatgag
     6481 atcgtttacg atctggcacc agaaaatcgt cagttgctgg cagaacgcga tcgcattcag
     6541 gcggcgcttg atgagtggca tcgcagcaat ccggggccgg taaaagataa agcggcctat
     6601 aaatctttcc tgcgtgaact gggctacctg gtaccgcaac cggagcgcgt gacggtggaa
     6661 accacgggca ttgacagcga aatcaccagt caggcggggc cacagctggt ggttccggca
     6721 atgaacgccc gctacgcgct gaacgcggcg aacgctcgct ggggctcact gtacgatgcg
     6781 ttatacggca gcgacatcat cccgcaggaa ggggcgatgg tcagcggcta cgatccgcaa
     6841 cgcggtgagc aggttattgc ctgggttcgg cgtttcctcg atgaatctct accgctggaa
     6901 aacggcagct atcaggatgt ggtggcgttt aaggtggtcg ataaacaatt acgcatccag
     6961 ttgaaaaatg gtaaagaaac cacgttacgt accccggcgc agtttgtcgg ttaccgtggc
     7021 gatgccgctg cgccgacctg cattttgctg aaaaataacg gcctgcatat tgaactgcaa
     7081 atcgatgcca acgggcggat tggcaaagac gatccggcgc acatcaacga tgttatcgtc
     7141 gaagcggcca tcagtaccat tctcgactgc gaagattcgg tcgcggcggt tgatgcggaa
     7201 gataaaatcc tgctgtaccg caacctgctg ggcctgatgc aggggactct gcaagagaaa
     7261 atggagaaaa acggtcgaca aatcgtgcgt aaactgaatg acgatcgtca ttacaccgcc
     7321 gccgatggct ctgaaatttc tctgcacgga cgctcgctgc tgtttatccg caacgtgggt
     7381 catttgatga ccattcctgt gatttgggac agcgaaggca atgaaatccc ggaaggcatt
     7441 cttgatggcg tcatgactgg cgcgattgcc ctctatgatt taaaagtgca gaaaaactcg
     7501 cgcactggca gcgtctatat tgtgaaaccg aaaatgcacg gtccgcagga agtggcgttc
     7561 gccaacaaac tgtttacccg cattgagaca atgctcggta tggcaccgaa taccctgaaa
     7621 atgggcatta tggatgaaga acgccggacc tcgctgaact tgcgtagctg tatcgctcag
     7681 gcgcgcaacc gcgtggcgtt catcaataca ggtttcctcg accgtaccgg cgatgaaatg
     7741 cattcggtga tggaagctgg cccgatgctg cgtaaaaacc agatgaaatc gacgccgtgg
     7801 atcaaagcct acgaacgtaa taacgtgctt tccagcctgt tctgtgggct gcgcggtaaa
     7861 gcgcaaatcg gtaaaggcat gtgggcaatg ccggacctga tggcagacat gtacagccag
     7921 aagggcgacc aactgcgtgc cggggcaaac acagcctggg ttccgtcgcc aactgccgct
     7981 acgctccatg cgctgcacta ccaccaaacc aacgtacaga gcgtgcaagc caacattgcc
     8041 cagaccgagt tcaatgctga atttgaaccg ctgctggacg atctgctgac tattccggtt
     8101 gctgaaaacg ctaactggtc ggcgcaagag atccaacaag agctggataa caacgtgcag
     8161 gggattctgg ggtacgtggt gcgctgggtg gagcagggga ttggttgttc aaaagtgccg
     8221 gatattcaca atgtggcgct gatggaagac cgcgcaacgc tgcgtatctc cagccagcat
     8281 atcgccaact ggttacgtca cggtattctg accaaagaac aggtgcaggc gtcgctggag
     8341 aatatggcga aagtggttga tcagcaaaac gctggcgatc cggcttatcg tccgatggcg
     8401 gggaatttcg ctaactcgtg tgcctttaag gctgccagcg atttaatctt ccttggtgtg
     8461 aaacagccaa acggctatac cgaaccgtta ttacacgcct ggcgtttgcg cgaaaaagaa
     8521 agtcattaat agcgtcaaaa aaagccccgg caagttttat tgtcggggct atgatagtaa
     8581 ctgcggtagt acatttatcc cggaaataac cggagcgatt atcagggttt aacaatatta
     8641 aatacccgta gcgcattact agcgggccta aaacaacaat atggatatct taacgatact
     8701 ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat acttgttatg ccagaggtag
     8761 caattgcgtt agcggcgtac tgttgctgta aagtataacg cctatatttt acagttcagt
     8821 cctcttttgc ttattacaac cttaagtata tcaagcatat aaagataata agagactgaa
     8881 caatatggtt acctggaccc aaatgtatat gccgatggga ggactggggc tatccgctct
     8941 ggtcgccctg atcccgataa tattcttctt cgttgcactc gcggtattac gtctgaaagg
     9001 acatgttgcc ggagcaataa cccttatatt atctctcctg attgcgatat tcgtctttaa
     9061 aatgccgatt gatatggcat tttctgctgc gggctatggc tttatttatg gattatggcc
     9121 aatagcgtgg attattgtcg cggcggtgtt cctctataaa ttaaccgttg ccagcgggca
     9181 gttcgatatt atccgcagct cggttatctc catcactgac gatcagcgtt tgcaggtgtt
     9241 actgattggt ttctcctttg gcgcgctgct ggaaggggcg gctggctttg gtgccccggt
     9301 ggcgattact ggtgcgctgc tggtgggcct gggcttcaaa ccgttatacg cggcggggct
     9361 gtgtctgatt gccaatactg cgccggtggc gtttggtgcg ttgggcgtgc cgattctggt
     9421 cgccggtcag gtaacgggaa tcgatccgtt ccacattggt gctatggcag gacgtcagtt
     9481 accgttcctg tcggttcttg tgccgttctg gctggtagca atgatggacg gctggaaagg
     9541 ggtgaaagag acgtggccag cggcgctggt tgcaggggga agcttcgctg tcactcagtt
     9601 ctttacctct aactatattg gtccggaact gccggatatt acttcggcgc tggtgagtat
     9661 cgtctcactc gctttattcc ttaaagtctg gcggccgaaa aataccgaaa cggcaatcag
     9721 catgggacaa tccgcaggtg cgatggtggt aaataagcca tcttctggcg gtccagtgcc
     9781 ttcagaatat agtctggggc aaatcattcg agcgtggtca ccgtttttaa tcttaacggt
     9841 gctggtcacc atctggacca tgaagccgtt taaagcgtta tttgctccgg gcggcgtgtt
     9901 ttattcactg gtgattaatt tccagatccc tcatttgcat caacaagtgt tgaaagcggc
     9961 acccattgtc gcccaaccaa cgccaatgga tgcggtgttt aaattcgacc ccctctcggc
    10021 tggcggcacc gctattttta ttgcggcgat tatctctatc ttcatcctcg gcgtgggcat
    10081 caagaaaggg attggcgtct ttgccgaaac gctaattagt ctgaagtggc cgatattgtc
    10141 gattggcatg gtgctggcgt tcgccttcgt caccaactat tccggcatgt ccaccacgct
    10201 ggcgctggta ctggcaggta caggcgtgat gttcccgttc ttctcaccgt tcctcggctg
    10261 gctgggggta ttccttaccg gctcggacac ttcttcaaac gccctgtttg gttcactgca
    10321 atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg ctggtggcag caaataccag
    10381 cggcggcgta actggcaaga tgatctcccc gcaatctatc gccgtggcct gcgccgcgac
    10441 gggcatggtg ggccgagaat ctgaactgtt ccgctacacc gtgaagcaca gtctgatttt
    10501 tgccagcgtt atcggcatta tcacgctgtt gcaggcgtat gtgtttaccg ggatgttagt
    10561 ttcgtaagtc aataatgccg gatgaaggta aatatcatcc ggctttccat ttgtttgtcg
    10621 tttatctcgc cagggaaccg tttttacgat tggcttctta atacatagtc tccgttttac
    10681 aatgcaaatt gtttaatatt tatacgtctg agcttctgtg ctggttataa agcacccact
    10741 ttcatgagat caattttagt atgttattac attgctacgt ctgttctttt catgacgaat
    10801 ggcgtttttt ctcttgtttg tatttttcac gagagcaatg agcagatcaa aattcccaaa
    10861 aacat
//
LOCUS       DAZUGK010000156        10426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZUGK010000156 DAZUGK010000000
VERSION     DAZUGK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.157"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..913
                     /locus_tag="ACHY5W_003526"
     CDS             8..913
                     /locus_tag="ACHY5W_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4448358.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(947..1549)
                     /gene="cobO"
                     /locus_tag="ACHY5W_003527"
     CDS             complement(947..1549)
                     /gene="cobO"
                     /locus_tag="ACHY5W_003527"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS4448359.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1559..3211)
                     /locus_tag="ACHY5W_003528"
     CDS             complement(1559..3211)
                     /locus_tag="ACHY5W_003528"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS4448360.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3319..4599)
                     /locus_tag="ACHY5W_003529"
     CDS             complement(3319..4599)
                     /locus_tag="ACHY5W_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFS4448361.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFIPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4760..5077)
                     /locus_tag="ACHY5W_003530"
     CDS             complement(4760..5077)
                     /locus_tag="ACHY5W_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFS4448362.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5074..6444)
                     /locus_tag="ACHY5W_003531"
     CDS             complement(5074..6444)
                     /locus_tag="ACHY5W_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFS4448363.1"
                     /translation="MARSFKVLSPTAILGYEFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6448..7688)
                     /locus_tag="ACHY5W_003532"
                     /pseudo
     CDS             complement(6448..7688)
                     /locus_tag="ACHY5W_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
     gene            complement(7688..9133)
                     /locus_tag="ACHY5W_003533"
     CDS             complement(7688..9133)
                     /locus_tag="ACHY5W_003533"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFS4448364.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVEIFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRSLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9152..>10426)
                     /gene="glmL"
                     /locus_tag="ACHY5W_003534"
     CDS             complement(9152..>10426)
                     /gene="glmL"
                     /locus_tag="ACHY5W_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFS4448365.1"
                     /translation="SSGEGFFSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAA
                     MGLVPSITLETAKVTAHSAGAKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEE
                     SYGLNNARVLAESKLDCAIIYAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAA
                     RQAICDIFLKRIVKGKGLDVIVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDM
                     GGATTDVYSACANTLSPDTVLHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNF
                     AQQPAQLDAFYHYLRHLVAHPDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCT
                     CVGNVDLQMGRDLTTVRKVVGSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEY
                     YRDSRGLLPLLANVARVDPLAAARTSIQCLTL"
BASE COUNT         2458 a         2643 c         2636 g         2689 t
ORIGIN      
        1 cggcattatg cgagggaaaa tacctaaaac ggaactgctg gtgacttttg aggtcgtcgc
       61 acgtcatgaa agttatacca gagcggcaga ggagttagcg ttaacccaaa gtgcagtatt
      121 cagacaagta agtgcgctgg aggaattttt acgtactccg ttattttcac actctaaaaa
      181 acgtattttt cttaatgatg caggtaagta ttacctgggg atagtaaaag aaacattaaa
      241 taagctagaa cgcgacacca ataccattat gacctggcag cctacggttc aggttattga
      301 acttgccgtt aatcctactt ttagtaccca ctggttaatc ccaaatttac atgaattcac
      361 aaagttacat cccgatatta ttgttaatat tcattcttta gctaataacg gcgattttct
      421 taatcgggag tacgatgctg tcattatgcg cgaaaatttt tgcgcgccct gggcggaagt
      481 agaatatctc tttgatgagg agattttgcc ggtatgcagc ggcagtttgc ttgcaatgtc
      541 cgatcaaaaa ctgtcagtgg cagaactgct tactgagtta ccgcttctgc atcaaagcac
      601 ccgtattact ggctgggaag agtggtttgc tttatctggc gttagcagtc cgctggtcaa
      661 taacggacca cgtttcgatt tgctttcaat gctgattgcc gccgtgcgct ctaatctcgg
      721 cgtggcgctg ttaccacgtt tcgctattca acatgatttg gatagtggcg atatggtgat
      781 tccctgtgat gtgcctattc gtaccggtaa tcgttttatt atgacgtggc aggaagagaa
      841 gtctgactca ccgcacttgc agcaatttcg tgaatggttg ctggcgaaat cggtcgtacc
      901 tcaggagatg taaaacctgc gcctggcggg atgccaggcg cagttattac cagtcgatac
      961 ccggctgggc ctgaatgccg ttatcaaaag catgtttgac ggggcggatt tcgcttaccg
     1021 tatccgccat ttttaatatc tgactgtggc aaccgcgtcc ggtgacaatg acactttgtt
     1081 gtgccgggcg gttttgcaga gaagcgatca cttcttcggt atccaaataa tggtaagcca
     1141 gcatataggt cagctcatca agcaccacta agtcgtaacg cttgtcagcc agcatccgtt
     1201 tgctttccga ccagacctct ttcgccgcgt cgatatccgc ttgccggttc tgtgtctccc
     1261 aggtaaaacc ggttcccata atatgaaact caacccccag cggctggaga aggttatatt
     1321 cgccgttgtc ccattgcccc ttgatgtact gcgcaacccc caccgttttg ccatgaccca
     1381 ccgcgcgcgt cacagtacca aacgcggcag tggatttccc tttgccgttg ccggtgaaaa
     1441 caatcaagat cccttttttc tctatcgccg ccgcgacgcg cgtatcgacc tgttccttca
     1501 atttctgttg gcgctgcaca tgccgttcag ttgaaattcg cgcttccatt cattttcctt
     1561 aatgtcccgc agggcaactg gcgcagcggt gttgctgctc aaactgacta aagaaattat
     1621 tgcctttatc atctaccagg atgaatgcgg gcagattttc cacttccatc atccacaccg
     1681 cttccatacc cagctccggg tattccaggc aacgcagact tttgacatat tcttgcgcca
     1741 gtaacgcagc tgcaccgcca atacttccca gattgaagcc gccatgtttg tgacaggcat
     1801 cggtgacctg ctggctgcgg ttacctttcg ataacatcac cagactgccg ccagccgcct
     1861 ggaaggtatc gatataaccg tccattcgac cgcccgtggt tggacccagt gaaccgcagg
     1921 ccatgttctc cggtgttttc gccgggccag cgtaatagac aatatgatgc ttcaggtact
     1981 ccggcatcga ttcgccgcta tccagccgcg cttttatttt ggcgtgggcg atatcgcgcg
     2041 ccactacgat gggaccactt aacgacacgc gggtacctac cggtaatctg gcgagatcca
     2101 gcatcacgtc acgcaacgga cggtttagat ccagttgtac atgttgcgcg tgattttctt
     2161 cccgcaatga ggctggaata tattgccccg gattgtgttc gagtttttcc agccagatac
     2221 cgtgtttgtt aatttttgct ttgatattgc gatcggcaga acaggagagc gccatagcta
     2281 tcgggcaaga gccaccgtga cgcggcagac gaatgacgcg aatatcatga gcgaaatatt
     2341 tccccccaaa ttgtgcacca ataccaaatt gctgactggc ctcgagcaac gctttttcca
     2401 gctcaatatc acggaatgct tgtccctgct cgtttccgct ggtgggcaaa ttgtcgtaat
     2461 atttcgttga ggccagcttg gcaactttta aggtttgatc ggctgaaagc cctccgacga
     2521 caaaggcgat gtggtaaggc gggcaggcag cggtgcccag cgatttcatt ttttcgatta
     2581 aaaatgcggt cagtttttct ggttgcaata aggatttggt ttcctgataa agcgcggctt
     2641 tattggcgga gccgccccct ttattaacac agagaaaatg gtactcatca ccagcgacag
     2701 cgctgatatc aatctgcgct gggagattgg tttgcgtatt gacttcggta tacatatcca
     2761 gcggcgcatt ttgtgaaaag cgcagattat tttcctggaa agtactgtaa atgcctttgc
     2821 ttaaggcttc agcgtcgtta cctcccgtcc atatctgttg ccctttactg gcaacaatag
     2881 ttgcggtgcc ggtatcctga cagttgggta ataccccttt tgctgagact tcagcattac
     2941 gtaataactg caacgcaacg tatttatcat tactactggc ctgcggatcg ttaagaatgc
     3001 tggcgacctg ttgcaaatga gcggaacgta agaaaaaaga ggcttcataa aatgcctgcc
     3061 gggcaagcag cgttaatgct tcaggagcga tttttatcac ttcttcgccg tctaattccg
     3121 ttactgtaat atgttgatcg ctaattaatg tatattccgt accgtcttta ttttgtagaa
     3181 acggttcttg ccagataaat ggtttggaca tatttcactt ttcaccttaa acgttagtgt
     3241 tacttaattg ccgtctaact tcatgatccg gcttataaaa atgcgtagtc attggcttct
     3301 gattgcttaa gatccccctc agggcagagc aacgttcgat tcattgggtt ttaatgcttt
     3361 accccgacag ccctcgccga aatgttcgcc ttcccaccga ctcaccatcg cagcgcccat
     3421 tgcgttgctc atgacgtttg tcgcggaacg ccccatatca aggaacggat caacgcccat
     3481 cagcaagatc agccccgctt ccggaatatt gaactggttg agcgtggcgg cgataaccac
     3541 cattgaggcg cgtggtacgc cagccattcc tttcgaggtc aacatcagga tcaacagcat
     3601 ggtgatttgc tcaccgatgg ataaatggat attgcaggcc tgggcgatga aaactgtggc
     3661 gaaggagcag taggccattg atccaacgag attaaatgaa tagccaatgg gtaagacaaa
     3721 gctggcaatt ttgggggaaa cgccaaattg ctccagtttt tcaagcgttc ccggaaaagc
     3781 cgcttcagaa ctggatgtgg taaatgccag cagggcgggt tccgaaaggg cgcgggtcag
     3841 gcgtctgatg caggggccga cataaacgat ggccagcccg ataagcagca cccaaagtaa
     3901 caacatggtg aaataaaatt cacccataaa gatcccggcg ctcaccataa ctgccagtcc
     3961 tcgttcagca atcaatgctg aaatagcggc gaatacggtc aggggagcga agagcatgac
     4021 gtagccagtg agctttaaca tggcatgtgc cagcgaatct aaggcgtgaa cgatggcgct
     4081 gcctttctcg ccaatcgccg tcaggctaca gccgaggaaa attgagaaca ccacgatttg
     4141 caaaatttca ttgtgcgcca tggcatcgac aatgctggtg gggaaagcat gcgaaataaa
     4201 tacttttagc gtaaagggtt ctgacgcgac cactcctgtg gtttcggcac cgtgtgcaac
     4261 aaaattaatt cctgtgcctg gtatgaagaa atttaccgtt atcaagccta aggcaattga
     4321 cagcaatgag gcgcaaataa ataaaaagag tgttttagaa aaaatacgac caagggcttt
     4381 ggcatcgccc attttagcaa tacctaccac cagggtagag accactaacg gagcgataat
     4441 catttttatc agtcgtaaga aaatcgtcgt gaatatcgat atttcttgcg catagagttt
     4501 tgctgtttcc ggtgaagcag tgttattgag cactacgccg ataatcattc caagtaccag
     4561 cgccaaaaga atcatcgtgg ttaaacttat tttcttcatt ttttcctcca tcaggtgcac
     4621 ggcgataaat cagaatcagg agaagtagat gcctgaatag agattcaggc atctacctgc
     4681 tattacaacc ttacaaatag ctgaagtgtt gaactgcaat aagtgacggg atgtcagttg
     4741 gctaaaagta ttcggatttt tacaaatcga aactcatatt taataaaggt gcatgttgct
     4801 gtgcaccata gacatcattg tcacctacat tcccggagat aatatctcgt ggcatgacga
     4861 tttttactgc gttagaagga tcaaaccaga caatacgatg aataaagtca ggttcaacat
     4921 gatataagcg cgcaatcagt tctggggtta attgccctga tgatttaact cgctcatagt
     4981 tttctttcgt tttaaataaa atatctaaaa ccagttcgta aggtccggca tttttcgaac
     5041 gaatgacttg cgccagtgag acaattgact gtttcatgcc tgaactcctt ctggcgtcac
     5101 ctgttcaata tggaaatcaa aacgcagagc gtcgttggct tcaatcaggt gatatatgga
     5161 aaattcgtat accggcccgc cctggatatc agaaggggag aacgggaacg ccaggttgcc
     5221 tgcggtagcg atgcggtttt catagccgta gtgcagcatg gtagagcgca ccagcgagca
     5281 aacgctattg gcaatttcct gggtcggtgc aactacatca aggacaatcc ccagctcatg
     5341 cccggcagtt tgcatcggtt catggtcgcc catcaccccg tttttgccgt acaggtggaa
     5401 attgatgcga atgctgtcat cgtcgagcga caggttacgc gaaacgctgg ttttgacttc
     5461 atcgatgatt ttatcgatcc cagcgatcat gatcgggtcg cgcgttccgg cgatggtcag
     5521 acagcggaag cccacccgtc gcgcaccttc cagtttcagg gcatacggcg tttcttcatg
     5581 cttagaaccg ctgacgtaca cttcgccgtc attgaccgct ttaaatgtgc agcctttcag
     5641 gttcaacacg ccgccaggtc ctggcagaaa gtagggatcg gatttctcat acagtgtgtg
     5701 tgcagctgct gacgtttcgg taaatttacg cttcggatta aatgtcttca gcgtaaagcc
     5761 gttgtcatca atgatgccca tcgcacagtc agagcctgag ccgggcgttg cggcgattgc
     5821 tgcgcattca aggattttcc cacaatgcag cgccagacct tcatcaaagc cttgcatgat
     5881 cgggagcgca gcaaagcagg ccgggtcgta agcgcgcccg cccagcacca cttgcgcgcg
     5941 ttctttcagc gcccgctgga agggttcgat gcccatttgc gcgacgatat acgtactttc
     6001 gtcaatcgcg tcgtgggtta acggcgggac aaaatccagc gccgtgattt tgccgttatc
     6061 cagcgcctgg tggacgatgg ctttgtcaac gtctgccggg atcaacgcca tcgagaacga
     6121 gaggtgctct tcctgggcaa tctcatgaat tatttctcgg caccactcca ggtgtggtgc
     6181 ggcaccagaa ccgccagccg ttccgatcac caccggtata ttattttgca cgcctgcggt
     6241 gatcatatag cgcagatcgc gcttgacccc tgcgcgatcg gtaaacggtt tgcctgcccc
     6301 gaggtagtgg gggcctggat cggaagagcc tgcatcaacg gcgatcagat cgggcgattc
     6361 tgccatcgct ttgcggaagc tctcttccgg gaattcatac ccgaggattg ccgttggcga
     6421 taagacttta aaagaacgag ccatttgtta atccttagcc tgcaacagcg cgatagtacg
     6481 attcatttcg ttgaagacaa tatcgaggcc ttcgtcaaag cccatccctg gttttaccag
     6541 catacgcata gggcgagcgg caagggcgac gtggacacag gtgcgggcac tgacatcagt
     6601 ttcattgcag gtgccgccct ggtacgcttc catgctgtgg ctgttgcagt aaagaaccgc
     6661 atcgacgatg ttgtgaatac tgcccagatc cggggttttg atttgcacca tgtgacaact
     6721 ggcagcatca gtgaaatcaa caatatcctg gtaggtgtta caccattcat cggccacaat
     6781 tttcacgccg gaaccgaggc gcgtcagctc tttggtaatc gcggtcagca ggcgaatttg
     6841 atcgggcttg ttaccggcat cgaccggccc ttcgatgtag agcggcaggc cttgcgcttc
     6901 tttttccagg ctggcgatgt attgcgcaca gcgaagcgga tccatatcga agatcagacc
     6961 gatagtgccg tatacatcga tgtgcagggt tgggtggtag cgtgcgctgg tgcgcttgct
     7021 cagaatgcga tccgacaacc agcggacata ttcgcgcagt ttttcacctt taaagcccag
     7081 cttctcttcg acgttattaa tcagcgcatg gggcagcacg tcgatgcctt taaggatcat
     7141 cttatcgacg gcgatatatc gatcgtcgcc gctctgacca aataatggaa tggattccgc
     7201 cacgcgtggc aactgccatt catcacagac cacttcagtt ttcagacggc cggttgccag
     7261 cgcggtagca tcaagcagcg cctgtgataa tccgtagcgc acggcggtat gcagcaagtt
     7321 gccgtcaata cgcaatttgt cgaagaaacg ggcattcggc aggaaagcat ccacatcgcg
     7381 gcctaccagt aatggcttga tatggtcgtt gaggaacgga ataaagtgct ctgcgaggaa
     7441 cagtggatcg cgaccgccgc cccggaatac tgtacggcag tacagtcacc tacggcgacc
     7501 gcgccgtttt ccagaatcaa ctgtaccgat acgcactccc cggcctgacg aacggcgtta
     7561 aacccctgtg ttaccggctc cccggtataa aaaaagccgt catgacccgc tccgtttttt
     7621 atcgcctgct ggtcatcgaa atagaatgag gagtagccag cggtgaacag agcctgtttt
     7681 attttcatta tggtcttcct attaatttac tgtgggatac agcattgatg tcatctacaa
     7741 ccatctggaa tgtcggttgg cgtccttcac agcgtgcgcg ttctgcaacg taatcgtggt
     7801 ggaggtcgag aatatctttt ggcaatggaa cggctccggc ttccagtacg cgtatcgcgc
     7861 cggtgttgtc acggaccgga agaattttgc ccgcgttgca ggcggctggc gcaaacggca
     7921 catccagtac gcccgcttca aatgccagca ccgttccgcg cgcaatatca ccattaccca
     7981 gttcgaacac tttattcagt actgcgcgaa cctcgctctt aatcagttca atttccagtt
     8041 caaccgcagg gcacggcggg aatttctgct cattgaccat gttgagcatc tggcgggagg
     8101 ctttcagccc ctgaatgttg gcagcggcag tcgggatgcc ccaggcttcg tgcggacttt
     8161 tggtgatgac tttggtggca ccagacatcc ccgcgacggc tgcgccccag gagataatag
     8221 cgaacgcttt cgattcgtct tccggaaagc cgcccatcca ctggtgaaac acggtactca
     8281 attcatagtc ggtatagcca tagctctgaa agtattcgtg tgcaagttca cgcagagact
     8341 ggatcgccgc aacatcctgc gtcaggctgc cgacctggcc ataaccaacg gtgatagatt
     8401 tcacaccctg ttccagtgcc agcaagcctt cgattatcgc gatagagtgc gagataaacg
     8461 gcgggatcag cgtacccgtt agtgggccaa acggttcgcg gttgatgcgg atcccgtgtt
     8521 cttcatacat ccccattaaa cggtcgcagt actgccagtc gcggatcgat ttttccagcg
     8581 ttacgcgttt ggcatacgga atgttgtagg aaatgccacc gccttcataa ctggtaaagc
     8641 cgctggccat tgagatttca gccagcagac gcgcatctgg cgtgccgtgg cggatctgta
     8701 gcggtttttg cagggtttcg gtgaggctac ggcaagctgc gacaccatgg ttaactaccg
     8761 gtaaaccgtt cagtttagag gttccggctt cgatggattt tttaatccct acagccgctt
     8821 cttcgtaccg attcagacgg gtatatgcat cgatggtgct tggcagcaga tcgcactctt
     8881 cttgtagtgt tttcagcagt tcgatgtgtt cgtccatcag cgccacacct gcgcgcggtt
     8941 ggctaagggt tttaccttcc ttgtctgctt tcagcagcgc aagagagaag cgtttatgct
     9001 cagggatggt ttgttggtat ttaacgccat cttcaaagat ttcaacatcc ttaccggttt
     9061 cccaggtttt cagtacctgt tgccgctcgg tcatgaattc gtcatgggtt aatttcttat
     9121 ttcgaagttc cattgattgc agccttaatt gttatagggt taagcattga atactggtgc
     9181 gcgcggcggc cagcggatcg actctggcga cgtttgccag cagtggtaat agcccccggg
     9241 aatcgcggta atattcaaat tcggtcggta agagaatgcg tttaccgtcg tcgtttaact
     9301 cgcggtattt caaccagtga tgcatatcga actgactggc acgcgagagc catccgccgg
     9361 atccgacgac tttacgcacg gttgtcaggt cacgtcccat ctgtaaatcg acattgccga
     9421 cgcaggtgca gacctctttt ttggtgcccg catgtctttc ggtggcataa cccacacaca
     9481 gtccggccag gacggtatcg aaatatttct cttctgcatt tgcgggtaag tagtcgggat
     9541 gagcgactag atgacggagg taatgataaa aagcgtccag ctgtgcgggt tgctgagcaa
     9601 aattcacatt gactaactct ttcgcacttt caccaaccac cactgccgag acgcgcattc
     9661 ccagatcgcc ttccaccgtc cgttttacga agggttccgg tacgccgtgt aaaacggtgt
     9721 cgggcgagag cgtgttggca caggcggaat agacatcggt ggttgcgccg cccatgtcga
     9781 tcagcatgaa ctctttccag ctgtgatcga cgttactgat tgccttgacc agttcaaaga
     9841 ccgtccatgg tgtcggcatt ggttcttcgc ctgttttatc aacaatgaca tccagccctt
     9901 tccctttaac gatgcgttta aggaatatgt cgcaaatagc ctggcgtgcg gctaaagggt
     9961 tcggatggtc aagatcgggc agaacattgt cgacgatggt caggtcttta tgcccgagga
    10021 tctcctgcac ctcgtcctga atatcccggt ttccggcata aataatggcg cagtcgagtt
    10081 tggattctgc cagcacacgg gcgttattca gtccgtagct ttcttctccg ccgtcggtgc
    10141 cgccggtgaa caagagaata tcgggctgcg tttcttccag tgcctggata tcacggcggt
    10201 tcagcttgta ggcgtaatat tgcgcaattt tagcacccgc agagtgagcg gttactttcg
    10261 cggtttccag ggtgatagaa ggcaccaatc ccattgcggc aaccgccagc ccacctttgg
    10321 cagaggagga atacttcaat gcaacctcac cgctgtttaa cagggggagg gcattatcta
    10381 cgtttaacac ctggttcagg ctggagaaaa acccttcgcc agatga
//
LOCUS       DAZUGK010000157        10287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZUGK010000157 DAZUGK010000000
VERSION     DAZUGK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.158"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..142)
                     /locus_tag="ACHY5W_003535"
     CDS             complement(<1..142)
                     /locus_tag="ACHY5W_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFS4448366.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTA"
     gene            complement(229..297)
                     /gene="ykiE"
                     /locus_tag="ACHY5W_003536"
     CDS             complement(229..297)
                     /gene="ykiE"
                     /locus_tag="ACHY5W_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFS4448367.1"
                     /translation="MGWSLCFWHVSVKMTHDVVCYY"
     gene            434..955
                     /gene="fecI"
                     /locus_tag="ACHY5W_003537"
     CDS             434..955
                     /gene="fecI"
                     /locus_tag="ACHY5W_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFS4448368.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            952..1905
                     /gene="fecR"
                     /locus_tag="ACHY5W_003538"
     CDS             952..1905
                     /gene="fecR"
                     /locus_tag="ACHY5W_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFS4448369.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1992..4316
                     /gene="fecA"
                     /locus_tag="ACHY5W_003539"
     CDS             1992..4316
                     /gene="fecA"
                     /locus_tag="ACHY5W_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFS4448370.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEACVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRSYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQDKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4361..5263
                     /gene="fecB"
                     /locus_tag="ACHY5W_003540"
     CDS             4361..5263
                     /gene="fecB"
                     /locus_tag="ACHY5W_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFS4448371.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5260..6258
                     /gene="fecC"
                     /locus_tag="ACHY5W_003541"
     CDS             5260..6258
                     /gene="fecC"
                     /locus_tag="ACHY5W_003541"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFS4448372.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6255..7211
                     /gene="fecD"
                     /locus_tag="ACHY5W_003542"
     CDS             6255..7211
                     /gene="fecD"
                     /locus_tag="ACHY5W_003542"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFS4448373.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIYPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7212..7979
                     /gene="fecE"
                     /locus_tag="ACHY5W_003543"
     CDS             7212..7979
                     /gene="fecE"
                     /locus_tag="ACHY5W_003543"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFS4448374.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8537..8794)
                     /locus_tag="ACHY5W_003544"
     CDS             complement(8537..8794)
                     /locus_tag="ACHY5W_003544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448375.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAAAMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIINNNRSINWPV"
     gene            complement(8877..10073)
                     /locus_tag="ACHY5W_003545"
                     /pseudo
     CDS             complement(8877..10073)
                     /locus_tag="ACHY5W_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10116..10280)
                     /locus_tag="ACHY5W_003546"
                     /pseudo
     CDS             complement(10116..10280)
                     /locus_tag="ACHY5W_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         2246 a         2894 c         2798 g         2349 t
ORIGIN      
        1 aggcggtgta agtgaactgt agttgccatg ttttacggca atgagagcag agatagcgct
       61 gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag caggaaggac
      121 atctgatgga aatggaagcc acgcaagcac cttaaaatca ccatcataca ctaaatcagt
      181 aagttggcag cattaccgaa atccccatcc tttagggcag tgaggatgtc aataatagca
      241 gaccacatca tgagtcattt tgaccgacac atgccagaag cagaggctcc atcccatttt
      301 atacctaccc tataacactt agaaaaacaa catgttaaaa atgtctattg gaaacaattt
      361 tatttccaat tgtaatgata accattctca tattaatatg gctacgtgat aattaacttt
      421 tgatgcactc cgcatgtctg accgcgccac taccacagct tccttaacgt tcgagtcgct
      481 ttatggcaca catcacggct ggttgaaaag ctggctgacg cgcaaactcc agtctgcttt
      541 tgatgcagat gacattgccc aggacacttt tttgcgggta atggtcagcg aaacgctctc
      601 gacgatccgc gatcctcgct ccttcctgtg cactatcgcc aaacgcgtga tggtggacct
      661 gtttcgccga aacgcgctgg aaaaagcgta tctggagatg ctggcgctta tgccggaggg
      721 gggagcgcct tcacctgagg aacgcgaaag ccaactcgag accctacaac tcctcgacag
      781 catgctggac gggctaaacg gcaaaacacg tgaagcgttt ctgctttcgc aactggatgg
      841 tctgacatac agcgagattg cgcacaaact cggtgtttcc atcagctccg tgaaaaaata
      901 cgtggcgaaa gccgtcgagc actgcctgct gttccgtctg gagtatgggt tatgaatcct
      961 ttgttaaccg attcccgccg tcaggcgctg cgttcagctt cccactggta tgccgtgcta
     1021 agcggcgagc gcgtcagccc acaacaggaa gcgcgctggc aacagtggta tgaacaggat
     1081 caggataacc agtgggcctg gcagcaggtt gaaaacctgc gcaaccagct tggcggtgtg
     1141 cctggcgacg ttgccagccg ggcgttgcac gatacccgcc tcacccgccg tcacgtgatg
     1201 aaaggattac tgctgttgct cggcgctggc ggaggctggc agctctggca gtcggaaacc
     1261 ggcgaaggtc tgcgggcaga ttaccgcacc gccaaaggca cagtcagccg tcagcaactg
     1321 gaagatggct ccctgctcac gctgaatacc caaagcgcgg cggatgtgcg ttttgatgcg
     1381 catcagcgca ccgtccggct ctggtacggt gaaatcgcca ttactaccgc gaaagatgcc
     1441 ctgcaacgcc ccttccgtgt cctgacccgt cagggccagc tcactgcttt agggacagaa
     1501 tttaccgtcc gccagcagga taatttcacg cagcttgacg tgcagcagca cgccgtggaa
     1561 gtgcttctcg ccagtgcccc cgcgcaaaaa cgcatcgtga acgctggtga aagcctgcag
     1621 ttcagcgcct ctgagtttgg cgcagtgaaa ccgctggatg acgagagtac aagctggacg
     1681 aaggacatcc tgagcttcag cgataaaccg ctgggtgagg tgatagccac gctaacccgt
     1741 taccgcaacg gcgtgctgcg ctgcgatccc gccgttgccg ggctgcgcct gagcgggacg
     1801 ttcccgctga aaaataccga tgcgatcctg aacgttatcg cgcaaacgct tcccgttaaa
     1861 attcagtcta ttacgcggta ctggataaac atttcaccac tgtaaggaaa ataattctta
     1921 tttcgattgt cctttttacc cttctcgttc gactcatagc tgaacacaac aaaaatgatg
     1981 atggggaagg tatgacgccg ttacgcgttt ttcgtaaaac aacacctttg gttaacgcca
     2041 ttcgcctgag cctgctgccg ctggccggtc tctcgttttc cgcttttgct gcacaggtta
     2101 atatcgcacc gggatcgctc gataaagcgc tcaatcagta tgccgcacac agcggaatta
     2161 ccctctcggt tgacgccagc ctgacgcgcg gcaagcagag caacggcctg cacggcgatt
     2221 acgacgtcga gagcggcctg caacaactgc tggacggcag cggactgcag gtaaaaccgc
     2281 tgggaaataa cagctggacg ctggagcccg cgcccgcacc aaaagaagat gccctgaccg
     2341 tggtcggcga ctggctgggt gatgcgcgtg aaaacgacgt atttgaacat gctggcgcgc
     2401 gtgacgtgat ccgccgtgag gatttcgcca aaaccggcgc aaccaccatg cgtgaggtac
     2461 ttaaccgcat ccctggcgtc agcgcgccgg aaaacaacgg caccggcagc cacgacctgg
     2521 cgatgaactt tggcatccgg ggcctgaacc cgcgcctcgc cagccgctcg accgtcctga
     2581 tggacggcat ccccgtcccc ttcgcccctt acggtcagcc gcagctttca ctggctcccg
     2641 tttcgctcgg caacatggat gccattgacg tggtacgcgg tggtggtgcg gtgcgttacg
     2701 gaccgcagag cgtgggcggc gtggtgaact ttgttacccg tgccattccg caggactttg
     2761 gtatcgaggc gtgcgtggaa ggtcagctca gcccaacctc ttcacaaaac aacccgaaag
     2821 agacgcacaa cctgatggtg ggcggcacag cggacaacgg ttttggcacc gcgctgctct
     2881 actccggcac gcgcggcagt gactggcgcg agcacagcgc cacccgcatc gacgacctga
     2941 tgctgaaaag caaatatgcg ccggatgagg tgcacacctt caacagcctg ctgcaatatt
     3001 acgacggtga agccgacatg cccggtggcc tgtctcgcgc ggattacgac gccgatcgct
     3061 ggcaatccac ccgctcgtat gaccgcttct ggggtcgtcg caagctggcg agcctgggct
     3121 accagttcca gccagacagc cagcataaat tcaacattca ggggttctac acccaaaccc
     3181 tgcgcagcgg ctacctggag caagacaaac gcatcaccct ctcgccgcgt aactactggg
     3241 tgcgcggtat tgagccacgc tacagccaga tctttatgat cggcccttcc gcgcacgaag
     3301 tgggcgtggg ctatcgctat gtgaatgaat caacgcatga aatgcgttac tacaccgcca
     3361 ccagcagcgg gcagttgccg tccggctcaa gcccttacga ccgcgatacg cgttccggca
     3421 ccgaggcgca cgcctggtat ctggatgaca aaatcgacat cggcaactgg accatcacgc
     3481 cgggtatgcg tttcgaacat atcgagtcat accagaacaa cgccatcaca ggcacgcacg
     3541 aagaagtgag ctataacgca ccgcttccgg cgttgaacgt gctctatcac ctgactgaca
     3601 gctggaatct ttatgcaaac actgaaggct cgttcggcac cgtacagtac agccagattg
     3661 gcaaggctgt gcaaagcggc aatgttgaac cggaaaaagc gcgaacctgg gaactcggta
     3721 cccgctacga cgacggcgcg ctgacggcgg aaatggggct gttcctgatt aactttaaca
     3781 atcagtacga ctccaaccag accaacgaca ccgtcactgc acgtggcaaa acgcgccata
     3841 ccgggctgga aacgcaggca cgttacgatc taggtacgct aacgccaacg cttgataacg
     3901 tttccatcta cgccagctat gcgtatgtga acgcggaaat ccgcgagaaa ggcgacacct
     3961 acggcaatct ggtaccattc tccccgaaac ataaaggcac gctgggcgtg gactacaagc
     4021 caggaaactg gacgttcaat ctgaacagcg atttccagtc cagccagttt gcggataacg
     4081 ccaatacggt gaaagagagc gccgacggca gtaccggccg cattcccggc ttcatgctct
     4141 ggggcgcacg cgtggcgtat gactttggcc cgcagatggc agatctgaac ctggcattcg
     4201 gtgtgaaaaa catcttcgac caggactact tcatccgctc ttatgacgac aacaacaaag
     4261 gcatctatgc aggccagccg cgcacgctgt atatgcaggg gtcgttgaag ttctgatgtg
     4321 ttaacgcccg gcttgccggg cttttagctg gaatgtgatt atgttggcat ttatccgttt
     4381 tctttttgca ggcctgctgc tggtgatcag ccacgccttt gccgccacgg ttcaggacga
     4441 acacggcacg tttacactcg aaaaaacgcc acaacggatt gtggtgctgg aactctcgtt
     4501 cgccgatgcg ctggccgccg tggacgtcag cccgatcggt attgccgacg ataacgatgc
     4561 aaaacgcatc ctgcccgaag tgcgtgcgca cctgaaaccg tggcagtccg tcggaacgcg
     4621 cgcgcagccg agcctggaag ccattgccgc tctgaaacca gacctgatca ttgccgacag
     4681 cagtcgccat gcgggggttt acatcgcctt gcagcaaatc gcgccggtac tgctgcttaa
     4741 gtcccgcaac gaaacctacg ctgaaaattt gcaatctgcg gctatcatcg gcgaaatggt
     4801 gggtaaaaag cgagagatgc aggcacgtct ggaacaacat aaagagagga tggcgcagtg
     4861 ggccagccag cttcccaaag ggacacgcgt ggcctttggc acatcacgcg aacagcaatt
     4921 caacctgcat actcaggaga cctggaccgg cagcgtgctg gcctctctgg ggctgaacgt
     4981 tcccgctgcg atggcgggtg cgtccatgcc gtccatcggc ctggagcaac tgctggcggt
     5041 caatcctgcc tggctgctgg ttgcccacta tcgcgaagag agcattgtta aacgctggca
     5101 acaagatccg ctctggcaga tgttaaccgc cgcgcagaag cagcaggttg cttcggtcga
     5161 cagtaacacc tgggcgcgga tgcgcggtat ttttgctgca gagcgtattg ccgctgacac
     5221 ggtaaaaatc ttccaccatc agccgcttac cgttgtgaaa tgaccgcgat aaaacacccg
     5281 gtgctgctgt gggggcttcc cgttgcagca cttattatta ttttctggct gagtctgttt
     5341 tgctactcgg ccattcctgt ttccggagca gatgcaaccc gcgccctgct gcctggacac
     5401 acgccaacgc taccagaagc gctggtgcaa aaccttcgtt tgccacgaag cctcgtcgcc
     5461 gttctgatcg gcgcaagcct ggcgctcgcg ggcacgctgc tgcaaaccct gacccacaac
     5521 ccaatggcct ctccttcact gctcggcatt aacagcggcg cggcgctggc tatggcgctt
     5581 accagcgcgc tgagtccgac gccgattgca ggctattctc tgtcgttcat cgcggcatgc
     5641 gggggcggcg tgagctggct gctggtcatg accgcaggag gaggatttcg tcatacccat
     5701 gacagaaaca aactgatcct cgcgggtatc gcgctgtcgg ccttttgtat gggccttacc
     5761 cgcatcaccc tgctgctggc cgaagatcat gcttacggca tcttttactg gctggcaggc
     5821 ggagtgtccc acgcccgctg gcaggatgtc tggcagctct tgccggtggt ggtcactgca
     5881 gtccctgtcg tgttgctgct ggcgaatcaa ctgaacctgc tcaacctcag cgacagcacc
     5941 gcccatacgc tgggagtgaa cctgacgagg ctacgtttgg tcatcaatat gttagtgctg
     6001 cttctggttg gtgcgtgcgt tagcgtggca ggtccggtgg cgtttatcgg tctgctggtg
     6061 ccacatctgg cgcgcttctg ggcaggcttc gatcagcgca acgtactgcc agtgagcatg
     6121 ctgctggggg ccacgctgat gctgctggca gatgtactcg cacgcgcgct ggccttcccc
     6181 ggagatctgc ccgcaggcgc agtgctggcg ctgattggca gcccttgctt tgtctggctt
     6241 gtgaggaggc gaggatgaaa attgcgctgg ttattttcat cacccttgcc ctggcgggct
     6301 gtgcgctgtt atcactccat atgggagtga tccccgtgcc gtggcgcgcg ctgctgaccg
     6361 actggcaggc cggacgcgag cattattatg tattgatgga gtaccgactg ccgcgcttgc
     6421 tgctggcact gtttgtcggt gcagccctcg ccgtggcggg tgtgctgata caggggattg
     6481 tgcgcaaccc tctggcatca ccggatattc tcggcgttaa ccatgccgcc agcctggcct
     6541 ctgtgggggc tctacttctt atgccgtcac tgcccgtgat ggtgctgccg ctgctggcct
     6601 ttgcgggcgg catggcgggg ttgatattgc tgaagatgct ggcaaagacc caccagccga
     6661 tgaagctggc gctcaccggc gtggcgcttt ctgcatgctg ggccagcctg acggattatc
     6721 tgatgctctc gcgcccgcag gatgtgaaca acgccctgct gtggctgacc ggcagcttat
     6781 ggggccgtga ctggagcttt gtgaagattg ccatcccgct gatgatttta tttctgccgc
     6841 tgagcctgag tttttgccgc gatctcgacc tccttgcact cggcgatgcg cgcgccacca
     6901 cgctcggtgt gtcggtgccc catacccgat tctgggcttt gttactagct gtcgccatga
     6961 catctaccgg cgtggccgcc tgcggcccga ttagctttat tggtctcgtg gtgccgcata
     7021 tgatgcgtag catcaccggt ggacgtcacc gcagactgct gcctgtttcg gccctgacag
     7081 gtgcgttgct gttggtggtt gccgatctgc tggcgagaat tatttatccc ccactggagc
     7141 tcccggttgg cgtgctgacc gccattatcg gtgcgccgtg gtttgtctgg ttgcttgtga
     7201 gaatgcgata aatgacttta cgaactgaaa atctgacggt cagttacggg acagacaagg
     7261 tacttaacga cgtttcactc tcactgccaa cggggaagat caccgccctg atcggtccta
     7321 acggttgcgg gaaatcgacg ctgttaaact gtttttcgcg gcttttaatg ccgcagtctg
     7381 gcaccgtatt tctcggcgat aatcccataa atatgctctc atcgcgccag ttggcccgca
     7441 ggctttcgct gctgcctcag caccatttaa cgccagaggg gatcacagtc caggagctgg
     7501 tttcgtatgg tcgtaatccc tggctgtcac tctggggacg tctctccgct gaagacaatg
     7561 cacgagttaa tgtcgccatg aaccagaccc ggattaatca tcttgccgtt cgtcggttaa
     7621 ccgagctttc cggcggtcag cgccagcgcg catttctggc gatggtcctg gcccagaata
     7681 cgcccgttgt attacttgat gaaccaacca cctatcttga tatcaatcac caggtggacc
     7741 tgatgcggtt gatgggcgaa ctccggactc aggggaaaac ggtggtcgct gtgctgcacg
     7801 accttaatca ggctagccgg tactgcgatc aactggtggt aatggcaaac ggacatgtta
     7861 tggcgcaagg cacaccagaa gaggtgatga ccccaggatt gctgagaaca gtattcagcg
     7921 tggaagcgga aatacacccc gagccggtat ctggcaggcc gatgtgccta atgaggtaga
     7981 ttgcacaggc cgtaagaacc aaaccacgac tgaatgaaac tggactggcg ccagcaagcc
     8041 tgttcagact ggggctgaac ttttccggac tctgaaagat taccaatact catcgtccat
     8101 ccgcttgctt taggctgaca ggttcataat caacgcaaac cagagctgta caggcttggg
     8161 cgcggctttc aaaccagtcg tgatcacggc aatcaatttt gaactctgct taacggacat
     8221 ttctgtataa cccttacggc aacgaaaaac gcgaagttaa aattttagaa acccaaaaac
     8281 gtgacatgac taagtttaga tttcaggggg ggagatcaaa aaatttcgct ctgtgccaga
     8341 gcggacattc atggagctgg ttcattacca atgaggttgg gcttttgagg ataaatcaat
     8401 gatcagacgc caacgtaaat caaaagcaac cctggaacgg aattgctaat ccagtttctg
     8461 accatcgatt tttctaaaaa gtgtgcgttt gctactactt aggtaggtgc agctttctta
     8521 atcaccggca gccacgttat acaggccagt tgatggatcg attgttatta atgatatctt
     8581 catgagtcgg tgtctcaccc agcttaccga agctggcata aagtgaaggc agacgggccc
     8641 gtccttctcc ctttttcgcc agagggaaag cgcgaagcat ggcggcagcc tcccagtcac
     8701 aacaatagga tggtgtgcac ggctgctgac gccatgattc agcgatagag cctgaaaata
     8761 cggggtcaaa gccggtatca ttaaccagag tcatcgtgac ctgtactgcg gctggatgat
     8821 ctcctgctac tgcaactgct aggcgaccgc ggctcccttc aggtagtctg ttgtggtcaa
     8881 ctaaaactgg cctccgcgtt agagtttttc cagtatcggt tttctgattc gtttggtggt
     8941 aacccaccat tatattcgtg cggtcttagt gcgctgtaat atccaacgat atagtccgtt
     9001 attgcgtgag ctgcatcgct gaagcttaca tagcccgtcg ctggtaccca ttcgttcttc
     9061 agactcctga agaagcgctc cattgggctg ttatcccagc agtttccacg ccgactcata
     9121 ctctgcctga tccggtatcg ccacagtaac tgccggaact gcctgctcgt ataatgactg
     9181 ccttgatcgc ttggaacatc accccgacgg gcttaccacg ggtttcccat gccatttcca
     9241 gtgctttcat ggtaagcctg ctgtccggcg agaacgacat ggcccagccc actggttttc
     9301 ttgcgaacag gtcgagaaca acgacgaggt acgcccggcg cttacccgtc cagatatagg
     9361 tcacatcacc gcactacacc tgatttggct cggtcacggc gaactgccgt tcaaggtagt
     9421 tagggatagc aacatgttca tgaccaccac gtttataccg gtgagtcggc tgctgacagc
     9481 tgaccagccc cagctctttc atgagcctgc caacaagcca gcatcccatc tggtagcctc
     9541 tccgggttgc cattgtggcg atgcttcttg ctccgaccga accgtggctg atgccatgta
     9601 gcttaagtac ctgactgcgt aatacagccc gtctgccgtc tggtttttca ggacggtttt
     9661 tccagtatct gtagctgctg cgatgaaccc cgaacacatg gcagagtgtg accacaggat
     9721 aatgcactct gagtttcccg attatcgaga actgttcagg gagtctgaca tcaagagcgc
     9781 ggtagccttt ttaatatttc attctccatt tcaatgcgtt gtagcttttt cctcagctca
     9841 cgtatttcga tttgttctgg tgttatcgga gaggtttttg gtgttttgcc ctgacgctca
     9901 tcacgcagtt gtttgaccca tcttgtcatt gtggaaaggc caacatccat agctttggcg
     9961 gcatctgcca ccgtgtagtt ctggtcaaca accagttgag cggattcgcg tttaaactct
    10021 gcgctgaaat ttcttttttc attggagcac ctgtgttgtt ctgaggtgag catctcacct
    10081 ctgttcaggt ggccaaattc agtaaaccac tacacatccg tttcaggctc agcaaaagtt
    10141 aaggtttgtt gtagcaaggt cgtaaattca cggtatcgct gatcccactt ggaagctttg
    10201 taatttttat cttttgcaag agtatcaatt aattctctga gagtcagctt gcctgcttcg
    10261 acactatcca aaagagtcat cgtgact
//
LOCUS       DAZUGK010000158        10259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZUGK010000158 DAZUGK010000000
VERSION     DAZUGK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.159"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            247..1017
                     /gene="yjjP"
                     /locus_tag="ACHY5W_003547"
     CDS             247..1017
                     /gene="yjjP"
                     /locus_tag="ACHY5W_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFS4448376.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1008..1481
                     /gene="yjjB"
                     /locus_tag="ACHY5W_003548"
     CDS             1008..1481
                     /gene="yjjB"
                     /locus_tag="ACHY5W_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFS4448377.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1588..2127
                     /gene="dnaT"
                     /locus_tag="ACHY5W_003549"
     CDS             1588..2127
                     /gene="dnaT"
                     /locus_tag="ACHY5W_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFS4448378.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2130..2867
                     /gene="dnaC"
                     /locus_tag="ACHY5W_003550"
     CDS             2130..2867
                     /gene="dnaC"
                     /locus_tag="ACHY5W_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4448379.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2916..3410
                     /gene="yjjA"
                     /locus_tag="ACHY5W_003551"
     CDS             2916..3410
                     /gene="yjjA"
                     /locus_tag="ACHY5W_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFS4448380.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3664..5952
                     /gene="opgB"
                     /locus_tag="ACHY5W_003552"
     CDS             3664..5952
                     /gene="opgB"
                     /locus_tag="ACHY5W_003552"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFS4448381.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRH
                     HPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDPK
                     LNLVYIYGESLERTYFDNEAFPDLTPELGALKDEGLDFSHTQQLPGTDYTIAGMVASQ
                     CGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKSH
                     GFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVDT
                     HHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVSS
                     DHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNYL
                     GLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGSH
                     FRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELAL
                     STDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRYK
                     ADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITAK
                     AYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGNI
                     LGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHY5W_003553"
     CDS             complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHY5W_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4448382.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLNVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7251..8156
                     /locus_tag="ACHY5W_003554"
     CDS             7251..8156
                     /locus_tag="ACHY5W_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS4448383.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8327..9979)
                     /locus_tag="ACHY5W_003555"
     CDS             complement(8327..9979)
                     /locus_tag="ACHY5W_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFS4448384.1"
                     /translation="MEIFLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGALLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIVSLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGGRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2674 a         2632 c         2430 g         2523 t
ORIGIN      
        1 cgcccggtta tctattatcc tgactcgtaa aggtcaaaca ataaattcat tatagtgata
       61 atattcatgc gatagtttgt ttacggcgag agataattcg ctttttatca ccaagcgtct
      121 cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa acaaccgtgc
      181 ttcatgatac aaatggattc tcattatctg aacaatacgc atcacgtcta tgacaaaggg
      241 cgagttatgc aaactgagca acagcgagcc gtaacacggc tttgtatcca gtgtggatta
      301 tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc acgactgggt
      361 cgggcgctgg gaatggacag cgtcgaaagt tctatctctt cgaacgccat agtgctgaca
      421 actattaaag atgggcaatg cctgacatcg acacgaaaaa atcacgatcg cggcattaat
      481 atgcatgtgg tgactgaagt ccagcacatt gtgattctgg cggagcacca tctgctggat
      541 tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc aagatggctg
      601 gtagccttaa tggttggcct ttcttgcgcc tgtttctgta aactcaataa cggtggctgg
      661 gatggtgccg tcatcacctt ctttgccagt acgaccgcga tgtatatccg ccagctgctg
      721 gcacaacgtc atcttcatcc acagatcaac ttttgcctta ccgctttcgc cgccaccacc
      781 atttccggat tgcttttgca actccccact ttcagcaata cccccaccat tgcgatggcc
      841 gccagcgttc tgctgctggt gccgggcttt ccgttgatta atgccgtcgc cgatatgttt
      901 aaaggccaca tcaataccgg actggcacgc tgggcgatcg ccagtctgct gacactggct
      961 acctgcgtcg gcgtggtgat ggcactgacg atttgggggc tacgcggatg ggtgtgatcg
     1021 aatttctgtt tgcattggcg caggatatga tcctcgccgc cattcctgcg gtcggctttg
     1081 cgatggtgtt caacgttccc gtgcgggcgt tacgctggtg tgcgctgctt ggctcgatag
     1141 gtcatggttc ccgaatgatc ttgatgacca gcgggttgaa tattgagtgg tcaaccttta
     1201 tggcttctat gctggtcggc accattggta ttcaatggtc gcgctggtat ctggcgcatc
     1261 cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc gggcatatcg gcttataccg
     1321 caatgatttc ggcggtaaaa atcagccagt taggttacag cgaaccgttg atgattaccc
     1381 tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc ggtctttcca
     1441 ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc gcttaagcac
     1501 acggatgaga gacagcctcc tctcctccgt gtgttactat aaaagttatc tcccttctcg
     1561 ttcatcgttc catatttgag aaacagtatg tcttccagag ttttgacccc ggacgtcgtt
     1621 ggtattgacg ccctggtaca cgatcaccaa accgttctgg caaaagctga aggcggtgtg
     1681 gttgccgtat ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc acgcctggct
     1741 gaactgctgg cgctggaaga aaagctggcg cgtccgggaa gcgatgtcga tctggacgat
     1801 caactctatc aggaaccgca agccgctccc gttgctgtac ccatggggaa attcgccatg
     1861 tatccggact ggcaacccga tgccgatttt atccgcctgg cggcgctatg gggcgtggcg
     1921 ctaagagagc cggtgaccac cgaagaactg gcctcattca ttgcctactg gcaggcggaa
     1981 ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt
     2041 cgtgccagca acggcggact gccgaaacga gatgtgaata cggtcagcga acctgacagc
     2101 caaattccac caggattcag agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc
     2161 tgcaaaaaat gatgcctgcc catatcaaac ccgcattcaa aacgggtgaa gaactactgg
     2221 cctggcaaaa agaacaaggg gcgatccgct ctgccgctct cgaacgtgaa aatcgggcga
     2281 tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc actgcatcag aactgctcct
     2341 ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt
     2401 atgtcgaaga gtttgacggc aacatcgcca gctttatctt ttctggtaag ccaggaaccg
     2461 gcaaaaacca tctggcggcg gcaatctgca acgagctgct gctacgcggt aaatccgtat
     2521 tgatcatcac cgtggccgat attatgtcgg cgatgaaaga taccttcagg aatagcggta
     2581 ccagcgaaga acaactgctt aacgatctga gcaacgttga tctgctggtg atcgatgaga
     2641 tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc
     2701 gctcttcttc caaacgccca accgggatgc tgaccaatag taatatggaa gagatgacca
     2761 agttgctggg cgaacgcgtg atggaccgta tgcgcctggg taacagtttg tgggtgatct
     2821 tcaactggga tagctaccgt agccgtgtaa caggtaaaga gtattaatcc ccgtttctgg
     2881 cggtatactg cgacacaatt ccaccttgag taatgatgat gaaaactgtt aaacatcttc
     2941 tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga
     3001 aagatgcgct ctccagcgcc gccagcgaac ttggcaacca aaacagcacg acacaggaag
     3061 gcggttggtc gctcgcgtca ttaactaact tgcttagcag cggaaaccag gccttaagcg
     3121 cagataacat gaacaatgcc gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt
     3181 cggtaaccga tgccgaaaac atcaagaatc aggtgctgga aaagctgggc ctgaacagtg
     3241 aagagcaaaa agaagacacc aactatctgg acggtattca gggtttgctg aaaaccaaag
     3301 atggtcagca actcaatctg gataacatcg gaacgactcc gctggcagaa aaggtgaaaa
     3361 ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt catttcctga accgcgaaac
     3421 gccgcgtttt agctgttacc cggctgacgc ggctctctcc tgcccttttc cctgctcctg
     3481 gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtctg tttataaact
     3541 aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg cgacgccatt
     3601 acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc
     3661 ccgttgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg
     3721 aaagcgggac gtaacacctg gtggtttgca gccacattaa cggtgctggg gctatttgtc
     3781 gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat taacgacgcg
     3841 gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata catcctgccg
     3901 ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg cgctgggctg gatcctgcgc
     3961 cgtcgtcatc atccgcacca ttttggttac agcctgctgg cgctcttact ggcgctgggt
     4021 tcggtggacg ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     4081 gacggcgacc cggactttgc ggcttattat aaagagccat cgaaaactat ccctgacccg
     4141 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgag
     4201 gctttcccgg atctcacgcc tgaactgggc gcgttgaaag atgaaggcct ggatttcagc
     4261 cacacgcagc agctgccggg aacagattac acgattgcgg gcatggtggc ttctcagtgt
     4321 ggcataccgc tgtttgcccc ctttgaaggt aacgcctccg cttctgtttc cagcttcttc
     4381 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4441 cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca tggcttcgac
     4501 cacttatacg gctcagaaga gctgaaaagc gtggtagccg acccgcacta tcgcaacgac
     4561 tggggattct acgacgatac cgttctcgat gaagcgtgga aaaagtttga agagctttcc
     4621 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4681 tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca
     4741 ttcagcgcgg taagttgcag ccaggagaac atcgcgacgt ttatcaacaa aatcaaagcg
     4801 tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg accatttagc gatgaacaac
     4861 acggcgtgga aatacctcaa taagcaggat cgcaataacc tgttttttgt cattcgtggc
     4921 gacaagccgc agcaagagac gctggcagtg aagcgtaaca cgatggataa cggcgcgacg
     4981 gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg
     5041 cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccagat
     5101 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     5161 aacatgattg ccttctcggg tagccatttc cgtttgccgc tgctgttgcg ggtttcagac
     5221 aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac cgctgcgttt ccagctggcc
     5281 gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat ggcacaactc
     5341 tgggctccgg aactggcact ttccaccgac tggtgtgtct cgcaggggca gcttggcggt
     5401 cagcaaattg ttcagcatgt tgacaaaaca acatggaaga gcaagacggc atttaaagat
     5461 acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctgaagat tgttgataac
     5521 gatattcgct acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc agaagaggtg
     5581 aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgcgcagctg
     5641 ggcgatgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5701 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5761 gaacaaactc ttgtgctggg caatgaagtg accaccacca cactgcattt cgataaccca
     5821 accgatgccg acacactggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat
     5881 atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa
     5941 cgtgaagggt aattcctgac tgatatgtag gtcgcatagg gtgcgaccta cacaccttct
     6001 ccggcttaca acacattatt aagaaacaaa aaaaacagcg cccaacactt ccccggacgc
     6061 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacgccttt aattaactca
     6121 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     6181 gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac tttagcaaaa
     6241 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6301 tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga
     6361 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6421 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc
     6481 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6541 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6601 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6661 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6721 atgttcgcca ccggcacgtt caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6781 actgaaattt tttcacagca atttgtacgc ccgcttttac acgccgggca ttgctgacaa
     6841 gaaatatacg gaattaccgc aacttgctgt ccgatattaa aattgtgaat gttttttcct
     6901 gattcaataa tctccccaca tatttcatgg cctaaaacgc gtggataact aaaaaatggt
     6961 tgattgcccg cccaggcatg aatatctgta ccgcaaatcc cgacagtttt aatttttatt
     7021 aatgcttcat tatcgcctgg caccggtctt tcccggactt cccatactaa attctttatt
     7081 tcctgacaaa ttaatacttt cattgtggac atgattcacc gctcctttta ttttatgact
     7141 ttcagatatc gtcgaattta taagagcgcc tgaatttacc cataaaatat gcggatgatc
     7201 acgcataaaa atgttttaaa tcggttttta atgccgaact ggagggaaga atgagtcgtt
     7261 cgcagaattt acgtcataac gtcataaatc aggtaattga agatatggcg aggggcaata
     7321 ttccttctcc gctgccttca caaagcgggc tggcagaaat gtataacatc agtcgcacta
     7381 cggtacgcca catactccag catttaagcg catgtggcgt gctcaccctg gtggggaaaa
     7441 attatgttat cgcccgcaaa cccgaacaga acgatggatt tgattgcatc acggcctcgc
     7501 tgaaagagca aaaccgtctt ttcgagcagg catttttcac catgattaac ctgcgtcagt
     7561 tacgcgccgg agaaagcttt tccgaacttc agctggcgcg agcggcaggc gtcagtccgg
     7621 tagtggtgcg agaatatctt ttgaaattcg gtcgttataa tcttattcaa agcgaaaagc
     7681 gcggccagtg gagcatgaag cagttcgatc aatcctacgc cgagcagctg tttgagctac
     7741 gcgaaatgct cgaaacacat tccctgcaac actttctcaa ccttcccgat gacgatcccc
     7801 gctggttgca ggcgaaaacc atgctcgaac gtcacaggac attgcgcgac agcattggca
     7861 gcaactttcg gatgttttct caattagacc gtgattttca tgccatgctg ttatccgctg
     7921 ctgacaacat cttttttaat caatcacttg aaattatctc ggtgatcttt cattttcact
     7981 atcaatggga tgaaagcgat ctcaagcagc gcaatatcat tgctgttgat gagcatatga
     8041 ccattctcag cgccctgatt tgccgtagcg atttagatgc aataacggcg ttgcgaaacc
     8101 atctggatac cgccaaacaa tcaatgattc gttccatcag gcaacatcac cgataaatta
     8161 agtacagatt aaaaacattg attcttggtt tatatcacta tgttgtatta atgcagtatt
     8221 aattcaggcg attgttctgc ccataaggaa atacataggg aataaacgat gctggaagta
     8281 ttactctcag ccatcattta actccctatt actggcagcg ataaaattat cggctatcca
     8341 tgattaacgt ttccagcaca tctaattcac tgactatttt tgccagtgtt tcatcactga
     8401 cttcttgccg tgcacgcatc tggtaaatag ccgcacgcct ggcatttaac gctgccagat
     8461 taaatgttct ttccaacttc gcgcgctgca tatgtaaaga attatcattt tccgacatat
     8521 catcttcgct acgccgcata taaccgataa ctctggcccc tgtttccata atcaactcgt
     8581 tgtcgaggtt ttctgtactg gtgcgtagca aacgttgctc agtcttttgc acactgagta
     8641 tggccatttt ggccataaac agatcggcag cttcctcttc tttttgccgg ttatcgctga
     8701 aaatgcccac atttttcccc agtagtaacg gcagaataat aatgccgcaa accagcgaaa
     8761 agaggatcac acctgtggcg agaaatacca gtaagtaacg cccgggaaaa ggtgtgccat
     8821 ccggcagcaa taacgggata gaaagcaccc ccgcaagcgt gatggctccg cgcccccccg
     8881 caaaggtcgc cagcagcata tgacgcgttt tatatccagc aaacagcatc ggatgtttgc
     8941 cgggtcgttt acggctatac agacgcatca gccaaagcca gcccaggcgc acaaccatta
     9001 acgccaggta gatccaaatg acatcaagga gcagcgatcc ggtgtctacc agtggatcgt
     9061 tttctgcacg gatcagtgat tccgaaataa tgcccggcag ttgtaagcct aaaatcagaa
     9121 acaccatgcc gttaaagaca tattcaagca tcgaccagct acttttagct ttaattctca
     9181 ggcggatagg cgcgtggtgg actacgccaa tgcgactggt tgttaacccc gcggcgacag
     9241 cggcaagaat gccagataag ccaaaatgtt ccgcaaccag ataaacaata aacggcaaca
     9301 gaagcatcaa tacaatctga atggaaggtt catcgtgagc atatcgactg atcaacagca
     9361 gccctttgcc atataaccag gtaacagcga tccccacggc aataccaccc aacgcaacga
     9421 caaaaaacgc aatggttgcg ccatgaacgc tgaattccat cgttcccatc gtcactgcaa
     9481 cagcaaattt caacgccacc aggccggaag catcattcat caacgcttcg ccttcgagca
     9541 cttcctggat gtttttgctg atacgccctt tcccgacaat ccccagcaac gccacggcat
     9601 cggtaggcga caacacggcg gctaatgcga aagcagcgat gagcggcaca ttcggtaaga
     9661 gtaaatgaat cacatagccg acaccaaaaa tagtgattag caccaacaca agcgccaggc
     9721 tgacaatttc ccgactgtgc ttgatcaatt ctttaggttg tatttttgag ctttcagcaa
     9781 acaacaaggg gggtataaac aataagagaa aaagttcagg attgaattcc acatgcagac
     9841 caaatatggg ttgggccagt aaagcgccag cggcaatctg gattaaagga agtggtatct
     9901 gaacaggaga cagacgcgta agcacgctgg ttaatgatac taccaacgta agaattaaaa
     9961 ttgttaaaaa tatttccata taataaacag gcttcctcat tcataaataa tggatattat
    10021 taatttaact tcataaaagc gccctggtta gcacttaacg ttaatcatta atcacaacgt
    10081 tcactcacat gctcaggagg aaaccagtga gatgagtttt ccagttgata acaaattcat
    10141 cacgtgaata tttactacct ttaaccgttg taacgaatca tccgttactc tttgatctcc
    10201 ttgccgaaca agttatttag tgaatgacgg ctttctttta acgtcagcat gaattatac
//
LOCUS       DAZUGK010000159        10253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZUGK010000159 DAZUGK010000000
VERSION     DAZUGK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.160"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(85..891)
                     /gene="trpA"
                     /locus_tag="ACHY5W_003556"
     CDS             complement(85..891)
                     /gene="trpA"
                     /locus_tag="ACHY5W_003556"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFS4448385.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(891..2084)
                     /gene="trpB"
                     /locus_tag="ACHY5W_003557"
     CDS             complement(891..2084)
                     /gene="trpB"
                     /locus_tag="ACHY5W_003557"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFS4448386.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2096..3457)
                     /gene="trpCF"
                     /locus_tag="ACHY5W_003558"
     CDS             complement(2096..3457)
                     /gene="trpCF"
                     /locus_tag="ACHY5W_003558"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFS4448387.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSFGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3458..5053)
                     /gene="trpD"
                     /locus_tag="ACHY5W_003559"
     CDS             complement(3458..5053)
                     /gene="trpD"
                     /locus_tag="ACHY5W_003559"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFS4448388.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIARL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5053..6615)
                     /gene="trpE"
                     /locus_tag="ACHY5W_003560"
     CDS             complement(5053..6615)
                     /gene="trpE"
                     /locus_tag="ACHY5W_003560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFS4448389.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLLNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6637..6730)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6707..6751)
                     /gene="trpL"
                     /locus_tag="ACHY5W_003561"
     CDS             complement(6707..6751)
                     /gene="trpL"
                     /locus_tag="ACHY5W_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFS4448390.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            6889..7770
                     /gene="rnm"
                     /locus_tag="ACHY5W_003562"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6889..7770
                     /gene="rnm"
                     /locus_tag="ACHY5W_003562"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFS4448391.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKVVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7767..8387
                     /gene="yciO"
                     /locus_tag="ACHY5W_003563"
     CDS             7767..8387
                     /gene="yciO"
                     /locus_tag="ACHY5W_003563"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4448392.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8415..>10253
                     /gene="yciQ"
                     /locus_tag="ACHY5W_003564"
     CDS             8415..>10253
                     /gene="yciQ"
                     /locus_tag="ACHY5W_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFS4448393.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVFDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDATLLNYHIVGVTRDGIPEP
                     WHHDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPTPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQILIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKQSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRS"
BASE COUNT         2401 a         2659 c         2706 g         2487 t
ORIGIN      
        1 agataaatat taaatgaatt taggattttt ccggcttcgt taaagaaagt taaaatgccg
       61 ccagcggaac tggcggctgt gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa
      121 agctttcagt gccgccagca ttttctctgg ctcattaata tgttgctcga tgattttaac
      181 aatggccgaa ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt ttacctgatc
      241 cggggcggaa ataccaaatc cctgcaatgg aggtgccgcg ttgtactctt tcagcttcgc
      301 aaccagatga ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc ccgctcgtga
      361 cagcaaatat gtgtaaccac gaccgtaaga ggctatctgg cgcagcaggt cgtcatcggc
      421 atttggcggg cagatgaaga taggtgcgac attatgacgc aacgcggcct ggcggaaggg
      481 cgcggactct tcaactggca catcggcaac cagcaccgaa tcgacgccga ctttttcgca
      541 ctgggcataa aactcatcaa tgcctttgtt aaacaccaga ttggcataca tcaacaggcc
      601 aatgggaatg gtcgggtgtt tctggcgaat cagtgccagc atttcaaaac attgtgccgg
      661 agtcacacct gccgcaaagg cgcgcagagt ggcgttttga atggtcgggc catccgccag
      721 tgggtcggag aaggggatac ctaactccag cgcgtcagca ccggcttcaa ttagcgtatc
      781 gataattttc aaggactgct caatgcccgg atccccgagc gtgacgaaag gaacgaatgc
      841 gccttctttg cgctccttca actgggcaaa cagagattcg tagcgttcca tcagatttcc
      901 cctcgtgctt tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg accggaaagg
      961 ttaaccacca gtagctgctc tttttccggg ttttcgcgca tcattttcag cgcatgggcc
     1021 agggcgtggg aggattccag cgccgggatg atcccttcgt gcaggcagag cgttttgaag
     1081 gcttcaaggg cttcatcatc ggtaattgac acgtaatcag cgcgtccagt gctgttaaga
     1141 tacgcgtgtt gcgggccgac ggacgggaaa tccagcccgg cggagatgga gtaagattct
     1201 tcaatctgcc cgtcttcggt ttgcatcatt ggcgctttca taccgaaata aatgcccacg
     1261 cgaccatgtt ttagcggtgc gccgtgctcg ccagtttcga taccgtgacc gccaggctcc
     1321 acaccaatca ggccgacgtc ggtttcgttg atgaaatcag caaacatgcc gatggcattc
     1381 gaaccgccgc caacacaggc gataacggca tccggcaggc gaccttctct ttccagaatc
     1441 tgtgctttgg tttcttcgcc aatcatccgc tgaaactcac ggacaatggt cggataagga
     1501 tgtgggccag ctgcggtgcc cagcatatag tgcgcggttt cataactgcc ggaccagtcg
     1561 cgcagcgcct cgttacaggc atctttcagc gttgcggaac cgctatgcac cgggatcact
     1621 tccgcaccca ttaagcgcat acgaaaaacg ttgggtgact ggcgctcgac gtctttggcc
     1681 cccatataaa tgcggcattt caggccgagc agggcgctgg caagggcaga ggccacgcca
     1741 tgctgaccag ccccggtttc ggcgatgatt tcggttttac ccattcgctt cgccagcaaa
     1801 gcctgaccga gcacctggtt ggttttgtgt gcgccgccgt gcagcaaatc ttcacgcttc
     1861 agatacagcg tggtgttcgt gccagccgtt atgttctggc atttggtcag cgcggttggg
     1921 cgtccggcat agtttttcaa aaggtcgtta aactgggcct gaaattcagg gtctttttgc
     1981 gcgctgacaa aagcttcttc cagctggcgc agggcaggca tcaggatttg tggcacgtac
     2041 atgccgccaa actcaccaaa ataggggtta agtaatgttg tcattgttcc tttccttaat
     2101 atgcgcgcag cgtctggaaa accgaggcca aaagacgtgc gtctttgatg cccggttgcg
     2161 actctacagc agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct tccacgcagt
     2221 tatctgcgcc taagcccccc gccagcagaa cgttgccaaa cgattgaccg tttaacagtg
     2281 accagtcgaa acgttgcccg cttccaccct ggccgttgtc taaaacatat ttatcaacgt
     2341 gctgaagctc gcgggcgggc agggtttcac cgacgcttaa tgctttccag atggcaacgt
     2401 gtgctggcag agcttcacgc agcgtatcga tatacagctg atcttcatta ccatgcagtt
     2461 gcactgccgc cagcgataac accttagctt tgtccaccac atcggcaata tcgtgattgc
     2521 ggaacacgcc aacatactgc aacggtgccg cagccatcac ttcctgcgcc tgttcaacgt
     2581 tgacgcaacg cggtgatgtc gcaaccaaaa tcaacccacc gtaaatcgcg cccgcgtcat
     2641 aagctgcttt agcatcttgc ccacgcgtca ggccacacac tttattctca cccagcaaca
     2701 cccgacgcac ggcggcgtgc aaatcgtcat gggccatcaa cgccgaacca atcagaaaac
     2761 cgttagcgaa gtggcttaac tcgcgcacct gagcgtaagt attgatgcct gattcgctga
     2821 ttaccgtcac gttgtgcccc agtttcggcg caagctcgcg ggtacggttg agatcaatcg
     2881 acaaatcacg cagatcgcgg ttgttgatgc caacgacctt tgcccccaat gcaatggcgc
     2941 gctccagttc ctcttcatta ctgacttcgg tcagcacccc catctccaga ctgtgagcga
     3001 cggctgcaag ctggcgatat tggtcgtcat ccagtactga aagcattaat aagcaggcat
     3061 cggcctggta atagcgcgcc agatagatct ggtaagggtc gataatgaag tctttacata
     3121 aaatcggctg cggggcgatt tggctgacga tggggaggaa atcaaagctc ccctgaaaat
     3181 atttctcatc agtcagcacc gaaattgccg aagcgtaatg tttataaatg gcggcaatgc
     3241 gtgctggatc gaaatcatca cggatcacgc cttttgacgg cgacgctttc ttgcactcca
     3301 gaataaacgc cgtgcgcgca ccctgtagcg catcataaaa atgtcgcgtg ctcggctgaa
     3361 cctcattctg aaaactggcc agcggttgct gctgtttgcg ggcttctacc caaatcgcct
     3421 tgtctgcgac gattttcgct aaaacggttt gcatcattta ccctcgtgcc gccagtgcgg
     3481 ttactctgtc gtaagcggaa ccactgcgca gtacctcaag aacggtttgc gcattggctt
     3541 gcagatcttc atggccatgc aggcgcatta acatggcgac attcgccgcg acggctgctt
     3601 catgggcggc gtcgccttta ccttgtaaca agcgtgttaa aatgtcacgg ttttcttccg
     3661 gtgttccgcc tgccagttgc tcctggtggt agggcgtcag gccaaaatct tcagcggtca
     3721 attgatagct cttaatttcg ccgtcatgca gctcggcaac gattgtcggc gcgtgtaatg
     3781 aaacttcatc catcccgccg ctgtgcacca ccgccgcgcg ttgatacccc agcacgcgca
     3841 aggtttcggc aatcggcagc accagttccg gactataaac accaattaac gccagcggcg
     3901 gatgcgccgg gttaatcaat ggccccagca cattgaacag ggtgcgggtt ttcagttgct
     3961 ggcgaaccgg catcgcgtgg cggaatccgg tgtgatactt cggcgcaaag aggaaacaaa
     4021 cacctaactc atccagcgcc tggcgcgatt tatcggcgtt catatcaaga ttaataccga
     4081 acgccgccag cagatccgac gaaccagatt tactggagac gctacggttg ccgtgtttcg
     4141 ccactttcag cccacaggcc gcggcgacaa acgcactggc ggtggaaata ttgatactgt
     4201 tgctgccgtc accgccagta ccgacgatat cagcaaacag ataatccggg cgcgggaacg
     4261 gcgctgcgtt ttccagtagc gcggttgctg ccccagcgat ctcgttcggg tgctcaccgc
     4321 gaattttcat gctcaccagc gccgccgcca gttgttccgg cttcagctcg ccacgcacca
     4381 ccgctgaaaa cagctggtga ctttcttgtt ggctaagcgt ctgcgcctga tacagttttt
     4441 ccagaatcgg ttgcagcgtg ttggctggct ctagcttctg ctgcgcccag gccagcgttt
     4501 gttccagcag gcgagctccc tgggtagtaa gaatggattc cggatggaat tggaatccac
     4561 aaacgcgatc cgcatcgtga cgtactgcca tcaccatgcc attaaaatgg gcgttgatgg
     4621 ttaaaccggc cggaatgtta ctgccaacca gcgagtgata acgcgccacc ggcagcgggt
     4681 ttgttaatcc ggcaaacatc gcctgaccgt catgttcaat gctcgacgct ttaccgtgga
     4741 gaatttcgcc cgcctgaccg acatagcccc cgtaagcttc gacaatcgcc tgatgtccga
     4801 ggcaaatgcc aataatgggc agcttgccac gcaagcgggt gaggagttcc ggcatacaac
     4861 cggcttcgct cggcacaccg gggccaggag aaagcatcag taccggattg ctcatcgtcg
     4921 ccaggcgtgc aattaaggtt tgcgccggga tatggttgcg gtaaatcacc acgttatgcc
     4981 cattgctgcg caactgatct gccaggttgt acgtaaaaga gtcgatatta tcgagcagca
     5041 gaatatcagc catcagaaag tctcctgtgc atgatgcgcg gtggcaatag cgcgcagtac
     5101 agcgcgggct ttattacggg tttcgtcggc ttccgactgc ggaacagaat caaggactac
     5161 gccagcaccg gcttgcacgg tggcgatacc gttttccacc agcgccgagc ggatcacaat
     5221 gcaggtgtcg agatcgccat gcgcggtgaa ataacctacc gcgccgccgt agctgccgcg
     5281 gcgacgacct tccgcctcgg caattaactg catagcacgc actttcggcg caccgcttaa
     5341 cgtccccata ttcatacagg cgcggtaagc gtgcagggcg tcgagatcgt ggcgcagctc
     5401 accgaccacg cgggagacca gatgcatcac gtaagagtaa cggtcaactt tggtgaggtc
     5461 ggcgacgtag cggctgccgg gggtgcaaat gcgtgccagg tcattacggg cgagatccac
     5521 caacatcaga tgttcagaaa gctctttatg atcggtacgc atttccagtt cgatgcggct
     5581 gtcgaggtct ctgtccagtg aaccatcggc acgacgaccg cgtggacgtg ttccggcaat
     5641 cgggtagatc tcaatctggc ggctggtggc gtcatacttg agcgaacttt ccggcgacgc
     5701 gccaaacagg gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg gattactctt
     5761 tttcagcacg taataggccg ccagcggtga cgggcagggc agagagaaac ggcgagatgg
     5821 caccacctgg aaaatttctc cggcgcgaat cgctttttgc aacaaacgca ctacgccacc
     5881 gaactcttca tcgctctggt tacattcaca acgcatatgc ggcacggaaa ccaccggcag
     5941 cggtggcgcg gtttcggtca gttgctggcg aagatcgttc aggcgagcag tgagacgttg
     6001 tttttcttct tcattcggag caaacaggct ggcctgaatg cgggtgcttt ttttctgatg
     6061 gtcaatcacc atcagcgttt cagcgagata aaaacagaaa tcagggcagt tattttccgc
     6121 tgacagttgc ggtaaatctt caaaccccgc cacaaggtca taagagaaca ggccgccgaa
     6181 gaacatggct tctcgttctt ccttaggtac attcaacaga ttcagcaata agcggaaagc
     6241 gtcaaaaacc gaaagggagc ataagcgagc gtcttcatcc agcagtggac taacaggggg
     6301 gaagcgcagc acacggcagt ttggtaattg ttcattttcc acacccgcag gcagggcgtt
     6361 atccagtagt gtcagcaggg cttcgccgtt gccggaaagg gactggattg tgacagtgtc
     6421 acctaaagct gtaatgcgta gcgcactgtc taccagcagc aggcttttta aatcatcttt
     6481 gctgtcgata tctgcggatt ccagcagcag cgttgccgga cgatccccac acaattggtg
     6541 aaaaagcgcg gtgggattgt cgcgataagc gccttcgcag gttagcagtt cgagagtcgg
     6601 tttttgtgtt tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc tcattaggcg
     6661 ggctgggtat ctgattgctt tacgcatggt gaatacactg cccgtttcag gaagtgcgcc
     6721 accaagcttt cagtacgaaa attgctttca ttgtcgatac cctttttacg tgaacttgcg
     6781 taccagttaa ctagttcgat gattaattgt caacagctca tttcagaata tttgccagaa
     6841 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
     6901 aattacgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
     6961 ccagaagcat tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc gatcaccgac
     7021 catgacacca cagctgcaat cgcgccagca agagaggaaa tttcacgttc gggactggcg
     7081 ctgaatctta ttcccggcgt ggaaatttcc acggtctggg aaaatcatga aattcatatt
     7141 gtcgggctga atattgatat tactcatccg ctaatgtgtg agttcctcgc gcagcagaca
     7201 gaacggcgca atcagcgggc gcagcaaatt gccgaaagac ttgaaaaagc gcaaatacct
     7261 ggggcgctgg aaggcgcaca acgactggcg cagggcggcg cagtgacgcg cggtcatttt
     7321 gcgcgtttcc ttgttgagtg cggcaaagcc agttcaatgg cggatgtctt taaaaagtat
     7381 ctggcgcgcg ggaaaaccgg atacgttccg ccacagtggt gtacaataga acaagctatt
     7441 gatgtcattc atcattctgg cggtaaggtg gtgctagctc atcctgggcg gtacaatctt
     7501 agtgctaaat ggctgaaaag attggttgcg cattttgccg aacacgacgg cgacgcgatg
     7561 gaagtcgcgc agtgccagca atcgcccaat gaacgcaccc agctggcgac acttgcgcgt
     7621 cagcatcatt tatgggcatc acaaggatct gattttcatc agccatgccc gtggatcgaa
     7681 ctgggtcgta aactctggct gcccgcaggc gttgaaggcg tctggcagct atgggaacag
     7741 ccgcagaaca ccacagagag ggaattatga gccagttttt ttatattcat cctgataacc
     7801 cacagcaacg tttgatcaac caggcggtgg agatcgtgcg taaaggcggg gtgattgttt
     7861 atccaactga ttccggctat gcgctcggct gtaaaattga agataaaaac gccatggagc
     7921 gtatttgtcg tattcgccag ctgccggacg gtcacaactt taccctgatg tgtcgcgatc
     7981 tttctgaact ctcgacctat tcatttgtcg ataacgttgc gtttcgttta atgaaaaaca
     8041 acacgccggg caactatacc ttcatcctga aggggacgaa ggaagtgccg cgccgtctgt
     8101 tgcaggaaaa acgcaaaacc atcggtatgc gtgtgccttc taacccaatt gcgcaggctt
     8161 tgcttgaagc actgggcgaa ccgatgcttt ccacttcgct aatgctgcca ggcagcgaat
     8221 ttaccgaatc cgatcccgaa gaaattaaag accgtctgga aaaacaggta gatttgatta
     8281 ttcacggcgg ctatctcggg cagaaaccaa caacggttat tgatcttacc gatgatacgc
     8341 cggtcgtggt gcgtgaaggc gtaggtgatg tgaagccttt cttataataa attgcgttcc
     8401 acggatggaa gactatggca gggaagtttc gctgcatttt gctgttgata gcagggctgt
     8461 ttgtatcatc tctaagttat gcagaaaaca cggagatccc ttcttatgaa gaagggatct
     8521 cgctctttga tgttgaagcc actctgcaac cggatggggt gttcgacatc aaagaaaata
     8581 ttcattttca ggcgcgaaat cagcagatta agcacggctt ttatcgtgat ttaccacgac
     8641 tctggatgca gcctgatggg gacgctacac tgctgaacta tcatattgtt ggcgtcaccc
     8701 gtgatggtat tcctgaaccc tggcatcatg actggcatat cgggttaatg agtattgtcg
     8761 tgggcgataa acaacgtttc ttgcctcaag gcgactatca ttatcaaatt cattatcagg
     8821 ttaaaaatgc tttcctgcgt gagggagatt cagatctgtt aatctggaac gtgactggta
     8881 accactggcc gtttgaaatc tataagaccc gattttcact caagttccct gatatcgcgg
     8941 gtaatccatt tagcgaaatc gatctcttta ctggagaaga gggcgacaca tatcgaaatg
     9001 gccgcatcct tgaggacgga agaattgaat ccagcgatcc gttttatcgt gaagatttta
     9061 cggtactcta ccgttggcct cactcgttac ttagcaatgc cccgactcca caaacgacga
     9121 atattttcag ccatcttctt ttaccctcca cgtcatcgtt gttaatttgg tttccgtgtc
     9181 tattcctggt ttgtggatgg ttatatctct ggaagcgcag gccgcaattt acgccggtag
     9241 atgtgattga aaccgatgtc attccgccag attacacgcc cggcatgtta cgtctcgatg
     9301 cgaagctggt ttacgacgat aaaggttttt gtgccgatat cgtaaatctg attgtgaaag
     9361 gaaaaattca tctggaagat cagtctgaca agaaccagca aatcctgatt cgtgttaatg
     9421 aaggcgtgac cagaaataat gcggtattac tgcccgcaga acagttatta ctggaagcgt
     9481 tatttcgtaa aggcgataag gtcgttctta cggggagacg caacagagtc ttacgcaggg
     9541 catttttacg gatgcagaaa ttttatctgc cgcgtaaaca gtcttcgttt taccggcctg
     9601 atacgttttt gcaatggggt ggactggcaa tattggcggt cattctttac ggtaacctga
     9661 gtcctgtagg ctgggcagga atgagtctgg tgggcgatat gtttattatg atctgctggc
     9721 ttcttccttt tttattttgt tcccttgagc ttttgtttgc ccgcgatgat gacaagcctt
     9781 gcgttaatcg tgccatcatc actttgtttt taccgctgat ttgttcaggc gtggcctttt
     9841 attctctcta tatcaatgtc ggagatgtat tcttttactg gtatatgcca gcgggttatt
     9901 ttagcgctgt tttcctgacc ggttatctca ctggtatggg gtatattttt ctgccaaagt
     9961 ttacccaaac tgggcagcaa cgttatgccc acggtgaagc tatcgttaac tatcttgcgc
    10021 gtaaagaggc agcaacacac agtgggcggc ggcggaaagg ggaaacacgg aaactggatt
    10081 acgcgttgct aggttgggca gtctcagcaa accttggaag agaatgggtc gcacgtatca
    10141 ccccatcact cacagcggct gttagtgccc cggaaattgc ccgtagtggc gttttgttct
    10201 cattacagat gcacctgagt ctgggggcca ataccagttt gttggggcga agt
//
LOCUS       DAZUGK010000160        10241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZUGK010000160 DAZUGK010000000
VERSION     DAZUGK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.161"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHY5W_003565"
     CDS             complement(<1..1816)
                     /gene="selB"
                     /locus_tag="ACHY5W_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFS4448394.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAAIEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLENNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
     gene            complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHY5W_003566"
     CDS             complement(1813..3204)
                     /gene="selA"
                     /locus_tag="ACHY5W_003566"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFS4448395.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEVELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3302..3910)
                     /locus_tag="ACHY5W_003567"
     CDS             complement(3302..3910)
                     /locus_tag="ACHY5W_003567"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFS4448396.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3981..5117)
                     /locus_tag="ACHY5W_003568"
     CDS             complement(3981..5117)
                     /locus_tag="ACHY5W_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4448397.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5120..5482)
                     /locus_tag="ACHY5W_003569"
     CDS             complement(5120..5482)
                     /locus_tag="ACHY5W_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS4448398.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKVTDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6019..7932
                     /gene="mtlA"
                     /locus_tag="ACHY5W_003570"
     CDS             6019..7932
                     /gene="mtlA"
                     /locus_tag="ACHY5W_003570"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFS4448399.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8162..9310
                     /gene="mtlD"
                     /locus_tag="ACHY5W_003571"
     CDS             8162..9310
                     /gene="mtlD"
                     /locus_tag="ACHY5W_003571"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4448400.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADINQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDEVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKSAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPYKNLIQGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9310..9897
                     /gene="mtlR"
                     /locus_tag="ACHY5W_003572"
     CDS             9310..9897
                     /gene="mtlR"
                     /locus_tag="ACHY5W_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFS4448401.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(9909..10118)
                     /gene="yibT"
                     /locus_tag="ACHY5W_003573"
     CDS             complement(9909..10118)
                     /gene="yibT"
                     /locus_tag="ACHY5W_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFS4448402.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         2420 a         2791 c         2643 g         2387 t
ORIGIN      
        1 gcaataaatg atcatttcca cgacgacgcg taaagccaat gcggtcaaaa tattccagga
       61 tttgaatcgc cagttttcgc ccaaccccta agcgatcgcg gaaatccgcc gcgcaggttg
      121 aaccacattc ctgatcgaga tcgcggatca tattggcaaa ctcgacaatc cgatcgttac
      181 ggtaataacg atctttgacg atcgcggtaa ttatcccctg ttgggccgcc tggcgtagcg
      241 tcagacgcat tgcctgctcg tcggttcccg tctcttttgc caggtcacgc acccaccacg
      301 gttcgtcacc aaacagcggc tctgcttttt gccagatggc ctgctgctct tcgctgaagc
      361 ccgctttgtg atctggcaga tgcagccagc cgtgatgact gaggatgtcg ccgctttcac
      421 gcatcttttc aatcagcaac agtaccagcg cttcatcttc cattggcaac gccatgcgcc
      481 gcagacgctc gcgcccagga ccaggttcat cgcgatgttg ctcatgataa gtcgctaatg
      541 tgtcaagaat tttccgctgc cagcgggcgg caactggcgc attcaacaag ctataaccag
      601 cctgaatata gcccggctgt tgcagcagtt cgcgcatccc ttcgccgttg agctggcgcg
      661 cccaggcgaa atccgcaagg ttaaccgctc cgcgttccag atgaacagat aacgcatcgg
      721 catcgctctg cgcccgtgca agagacgcca gccattgcag atattccggc ttacgtttac
      781 cgcgacgcgg cgggttaagc atcaccacac gcgcccccgc cagcgtattg cgggccgaga
      841 tatcgcgcaa cactaagcga tcgttatccg ccagccacag cggcgtgtcg aacaccagct
      901 cggcaaggtt attttccagc agcgaaacgc gtcccgtaac gtggctggcg gcgtggtgaa
      961 tatgcagcgg ctgccactgg gtcagcggcg tatgggtttg aagctcgaca atcacccgcg
     1021 tgaacggctc tggcggcaca tcggcaagca gccagtcgcc acggttaatc tgctcttttt
     1081 ccgcatcccc cgcgatgtta agcgcgatac gctggccggc atgagcggtt tctgttggct
     1141 ggttttgtgc atgcagtgca cgtacacgca tcggtttatt tacaccagtc agccatagtg
     1201 aatcgcctac cttcacttcc ccgcttaacg ccgtaccggt gacgaccagc ccggcacctt
     1261 ttacggtaaa tgcgcggtcg atcgcgaggc ggaaactatg ttggctggcg tgttcacgtt
     1321 ctggtaactg aagcagatgc tcgcgcaggg catcaattcc ccgaccttcg attgctgcgg
     1381 tgataaacag ttttgcctca gcaaaaccgt attcccgcag cacctccttt acctgacgtt
     1441 caacctcatc aacacgcgct tcgtccacgc gatcggcttt ggtcagcgcc actgtcagca
     1501 tcgggttacc ggtcagctgc aaaatcgcca gatgctcacg ggtctgtgcc atcacaccgt
     1561 catcgcacgc caccaccaac agcgcgtgat cgataccacc aacgcccgcc agcatgttgg
     1621 aaagaaactt ttcatgaccg ggtacgtcga taaaaccagg cacgcgacca tccggctgcg
     1681 gccagtaggc atagccgaga tcgatggtca tgccgcgctt tttttcttcc ggcagacgat
     1741 cggcatttac gccagtaatc gcctgcaata aggttgtttt gccgtggtca acgtgtccgg
     1801 cagtcgcaat aatcatttca acaacatctc caaaaaccgt tgctcatctt caaggcagcg
     1861 taaatccagc cacaatcgtc cgtcataaat acgaccaatc accggcactg gcaattcacg
     1921 ccagcgggcg gctaatgact caaggtggct accgcgtcca tcatggggtg taaacgttaa
     1981 tgccgcgctc ggcaggcgat caaccggcag cgaaccactg ccaatctgcg aaagacatgg
     2041 cataacctgt accgcaaact ccgcgccgta atgtgcggca aggggagcct gtaaacgttg
     2101 tgcctggatt tgaatgacct ctgcgctgcg ggtaagcagg cgcagggtcg gtaatttttc
     2161 actcagagct tcagggtgta aataaagacg caacgtggct tccaacgccg cgagggtcat
     2221 tttatccgcg cgtaatgcac gcttcagcgg gtggctttgc aggcgggcga tcatctcttt
     2281 tttaccaacg ataatccctg cctgcggccc gcctaacaac ttgtcgccgg agaagctcac
     2341 cagactgacg cccgccgcaa tcaactcctg cggcattggc tctttcggca aaccgtactg
     2401 gctaagatcg accagcgagc cactgcctaa atcagtcact acgggaacat ccagctcttt
     2461 gccgagcgcc accagttcaa cttcgtctat cgctttggtg aacccctgaa tgctgtagtt
     2521 actggtatgt actttcatca acagtgcggt attttcattc accgcctgac gataatcatt
     2581 cgcgtgcgtg cggttggtgg tccctacttc gtgtagtgtg cagcctgcct gacgcataac
     2641 atcgggaata cgaaacgcgc cgccaatctc caccaattcg ccgcgagaaa ccaccacctc
     2701 tttcccgctg gcagtggccg ccaacatcaa taacaccgcc gccgcattgt tattaacgat
     2761 acaggcatct tccgcgccgg taatgcggca cagcagctgc gccagcgccc gatcgcgatg
     2821 tccgcgtccg gcgtcgtcca gatcgtactc gagggtcact ggcgaacgca tagcctgcgc
     2881 aacggcttcc accgcggctt ccgcctgtaa agctcgccca aggttggtat gcagcacggt
     2941 tcccgtcagg ttgatcaccg gacgcagcgc gctctgcgcc tctttcgtca accgggcatc
     3001 gacttcttgc gcccagtttt cacaccacgc aggcagcgtc tggctgccac gaatcacttc
     3061 tcgcgcttcg tcgagcatct gacgcagcaa ttccaccacg cgggtgtgac cataagtatc
     3121 acgcaaagaa aggaaggagc tatcgcgcaa taagcgatca atagccggaa gttgactata
     3181 gagggaacgc gtttcggttg tcataggaaa cctggctgat caaggccctc tcacacggag
     3241 aagggcgttt aacataacca cggattgtaa cgtgagatgg gtcaggagga catatcgcgc
     3301 atcaagcctt tggcggttcg gtgcgagcaa aactttcgcg gctaaacagg ttttcgacca
     3361 gtttgactaa gtgtgggcgg tcgacacacc agcctggcgc aacgcggcgg aaattgagat
     3421 atccgacggc acaggcaatg gcgatagtcg ccagattgac agtatcggtt ttgagtgtgc
     3481 catcgaccag atatccttcc agcacatcca gactgcggtt gattttctcc cgctggcgta
     3541 acaattcatc ttcagactgc tgcgccgctg gacgcgcctg ttcacgcacc gataccagcc
     3601 cggcatccat aatgccatcc gccagtgcct caattttgcg cacccgcaac gactccagcg
     3661 gatcgcgcgg caacatcgcc ggagcaacat tcattaattc aatatattca gcgatgatcg
     3721 gcgaatcaaa ccagcattcg ccctcttcgg tcaccagcac cggcactttt cctaacgggt
     3781 taaattgcgc cacgccgttg tccgcgttat agggcagttc attaataaat tcgaaagtta
     3841 tgcccttttc taacaacaga atagaaagtt tgcgtacaaa cgggctggtg tagctaccga
     3901 cgagtttcat gccgagtcct ttgtgcgagg aaaaatatca gtatggctca ggctcacgca
     3961 aaacacaggt gatataagtc tcaatgatcc aaataaagat aatgcatcca gctggtcatt
     4021 cttagcagca cttttcgcat caccgaaaca tggctgaaat ggtcggagta aactgccacc
     4081 tgggcgtaga tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt
     4141 cccagcacac cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga
     4201 taagaaccgc ctggcacgac tggtaaaata ctggtcagtt tgccgtgaaa cacctgccca
     4261 ggtagcgcgt taaacaccac ttccgcatca tcaccaggtt tcagacgtaa cagcgagttt
     4321 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaaaaccat caccggacgc
     4381 agcggcaagg cagcggcgta tgtgcctggg cggatcagta cctgagtgac gtagccattg
     4441 ctcggtgcgc gaatgacggt ctgttcaagg ttatattttg cttctgtaag ttgcgctctt
     4501 aagctcacaa tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc
     4561 gcctgctccg ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca
     4621 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt cagataacgt
     4681 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtagtgtt ggcctgcgct
     4741 tctgtgagct gcgcgcgcag agtctttata ttatgcgtcg ccgtcatcag gtcagcctga
     4801 agtctgtcaa ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt
     4861 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     4921 atggggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     4981 aacaaaatca aaccactcac cagaaacacg ccacccagcg ccgccgtcgc cagcgtccac
     5041 tgattcaccg gaatacgaaa gattttaaag accgcccacg ccagcgccac gtaagttaaa
     5101 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     5161 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag
     5221 tcactttctc ctgcgacgca acatgtgact gcatacccca gccgcgctcc ggttgataga
     5281 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     5341 ccgccgtatg aatagcatcg gcatggggat ggttgcgctt tttggcaatc agataaggga
     5401 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     5461 acgcgaaata gtttagaaac atatttgcct cggattcacg tttatcagtg ttgttttttg
     5521 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     5581 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     5641 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgcaaga aaatatcttg
     5701 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt
     5761 taaatgccat ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cgttttgtga
     5821 tgaacgtcac gtcaattacc tctctacccc ctatatttat gtgattgata tcacacaaaa
     5881 ggccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     5941 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     6001 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     6061 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
     6121 gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg
     6181 atgatcactt atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
     6241 gaacgtggcg gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagacatg
     6301 ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
     6361 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
     6421 gcaggcatca tcgggatgat cctcgccatt ctggcatttc tcggcattgg cccgattgtt
     6481 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
     6541 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
     6601 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atccatattc
     6661 ttcctgattg aagctaaccc aggtccgggt atgggcgtgc tgctggcgta catgttcttt
     6721 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa ttattcactt cctgggtggt
     6781 atccacgaaa tttacttccc gtatgtgttg atgaatccgc gtctgatcct ggccgttatc
     6841 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
     6901 gcatctccgg gttctatcct tgctgttctg gcgatgacac caaaaggtgc ttatttcgct
     6961 aacatcgcgg gtgtgtgtgc ggcgatggct gtctcctttg ttgtctctgc tattttgctg
     7021 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
     7081 atgaaagctg agtctaaagg tgcatctccg ctgtctgcgg gcgatgtgac taacgacctg
     7141 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc
     7201 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc tgttactaac
     7261 agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc
     7321 gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac
     7381 agcggcctgt acaccagcct gactgaacgt ctggttgctg cccagcgcca tactgaaaac
     7441 gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc
     7501 aaactgggcg cggagaacat cttcctcggt cgcaaagctg ccaccaaaga agaagcgatt
     7561 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
     7621 atgttggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacat
     7681 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
     7741 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgct
     7801 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
     7861 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
     7921 ggtcgtaagt aatccaatcc caccctctcc acatggagaa ggtggggtta attgcctgat
     7981 gcgctacgct tatcaggcct acaggatgca tcacgatttg ttgaatttgc acgttcttgt
     8041 aggccggata aggcgcttac gccgcatccg gcgctgcccc tctcctcacg gagagggttt
     8101 gggtgaggga aaagcctcac cccagccctc tcgggtaaaa acattgatga aggttaatac
     8161 tatgaaagca ttacattttg gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct
     8221 ggcagacgcg ggtatccaac tgacgtttgc cgatatcaat caggtggtac ttgatgccct
     8281 gaatgcccgt catagctatc aggttcatgt ggtcggtgaa accgagcagg tagataccgt
     8341 ttctggcgtc aatgctgtca gcagcattgg tgatgaagtc gttgatctca ttgctcaggt
     8401 tgatttagtc actaccgccg ttggcccggt agtgctggaa cgtattgctc cggctatcgc
     8461 caaagggctg gtgaaacgta aagaacaagg taatgaatcc ccgctgaaca tcatcgcttg
     8521 tgaaaacatg gtacgcggta ccacgcagct gaaaggccat gtgatgaacg ccctgccaga
     8581 agacgccaaa gcgtgggtag aagaacacgt tggctttgtc gattccgccg ttgaccgcat
     8641 cgtaccgcct tcggcttctg caactaacga tccgctggaa gtgacggtag aaaccttcag
     8701 cgaatggatt gtcgataaaa ctcagttcaa aggcgccctg ccgaacatcc caggcatgga
     8761 gttaaccgac aacctgatgg catttgtcga acgtaaactc ttcaccctga acacgggtca
     8821 tgctataacc gcgtacctcg gaaaactggc cggtcatcag accattcgtg acgctattct
     8881 cgacgagaaa atccgcgcgg tggtaaaaag tgcgatggaa gaaagtggcg cggtactgat
     8941 caagcgttac ggctttgacg ctgacaagca tgcggcgtac atccagaaaa tcctcggtcg
     9001 ttttgagaac ccgtatctga aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa
     9061 actgagcgca ggcgaccgtc tgatcaagcc actgctcggt acgctggaat acggcctccc
     9121 gtacaaaaat ctgattcagg gaattgccgc tgcaatgcac ttccgcagtg aagatgatcc
     9181 acaggctcag gaactggcag cactgatcgc cgacaaaggt ccgcaggcgg cgctggcaca
     9241 gatttccggg cttgatgcca acagcgaggt tgtatctgag gcggtaaccg cttataaagc
     9301 aatgcaataa tggtggacca ggcgcaggac accctgcgcc cgaataacag attgtcagat
     9361 atgcaggcaa caatggaaca aacccaggcc tttgaaaacc gtgtgcttga gcgtctgaat
     9421 gctggcaaaa ccgtgcgaag ctttctgatc accgccgtcg agctcctgac cgaggcggta
     9481 aatcttctgg tgcttcaggt attccgcaaa gacgattacg cggtgaaata tgctgtagaa
     9541 ccgttactcg acggcgatgg tccgctgggc gatctttctg tgcgtttaaa actcatttac
     9601 gggttgggcg tcattaaccg ccaggaatac gaagatgcgg agctgctgat ggcattgcgt
     9661 gaagaactaa atcacgacgg caacgagtac gcctttaccg acgacgaaat ccttggaccc
     9721 tttggtgaac tgcactgcgt ggcggcgtta ccaccgccgc cacagtttga accagcagac
     9781 tccagtttgt atgcaatgca aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc
     9841 ctttcactga cagagctgat ttccagaatc agcttaaaaa aagccttcca aaagtaagca
     9901 acgtctgctt actgtccctc tacctgcttc ggccgataaa gccgacgata atactccaga
     9961 cgttgtagat ataacggcac gctttcatcg ggtattccgg acggaatcgc gttacgggga
    10021 ggaagttttt tcagatactc tcggaacgcc tggcttgatg ccatgaaatc tacggcttta
    10081 tcgataagaa gcggaacgtt ttcacctaat ttgcccatga tgatatctcc gtattacccc
    10141 gcgccgggaa tgcgcggccg ccaattttag tttaggttcc cggtaaagat gacattaagg
    10201 aaaacgtgct gaatcctcaa taatcatccg cttctgttta t
//
LOCUS       DAZUGK010000161        10216 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZUGK010000161 DAZUGK010000000
VERSION     DAZUGK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.162"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..5296
                     /gene="iatD"
                     /locus_tag="ACHY5W_003574"
     CDS             56..5296
                     /gene="iatD"
                     /locus_tag="ACHY5W_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
                     /protein_id="HFS4448403.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLIENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARIYLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDFEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSIQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNMSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPIVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYTANYPAHKQGTALRVQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLINHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVHLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     TMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSISLNLIQSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQGWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5451..6002)
                     /gene="kptA"
                     /locus_tag="ACHY5W_003575"
     CDS             complement(5451..6002)
                     /gene="kptA"
                     /locus_tag="ACHY5W_003575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFS4448404.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWANIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLE"
     gene            6252..6935
                     /gene="yjiH"
                     /locus_tag="ACHY5W_003576"
     CDS             6252..6935
                     /gene="yjiH"
                     /locus_tag="ACHY5W_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFS4448405.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            6932..7393
                     /gene="yjiG"
                     /locus_tag="ACHY5W_003577"
     CDS             6932..7393
                     /gene="yjiG"
                     /locus_tag="ACHY5W_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFS4448406.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7406..8578
                     /gene="iadA"
                     /locus_tag="ACHY5W_003578"
     CDS             7406..8578
                     /gene="iadA"
                     /locus_tag="ACHY5W_003578"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4448407.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8643..9554
                     /gene="hypT"
                     /locus_tag="ACHY5W_003579"
                     /gene_synonym="qseD"
     CDS             8643..9554
                     /gene="hypT"
                     /locus_tag="ACHY5W_003579"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFS4448408.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9547..9939)
                     /gene="iraD"
                     /locus_tag="ACHY5W_003580"
     CDS             complement(9547..9939)
                     /gene="iraD"
                     /locus_tag="ACHY5W_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFS4448409.1"
                     /translation="MMRQSLQAVLTEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9936..10019)
                     /gene="idlP"
                     /locus_tag="ACHY5W_003581"
     CDS             complement(9936..10019)
                     /gene="idlP"
                     /locus_tag="ACHY5W_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFS4448410.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(10135..>10216)
                     /gene="ytiD"
                     /locus_tag="ACHY5W_003582"
     CDS             complement(10135..>10216)
                     /gene="ytiD"
                     /locus_tag="ACHY5W_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFS4448411.1"
                     /translation="FHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         2669 a         2593 c         2532 g         2422 t
ORIGIN      
        1 atatctccgt gctagaaaaa acaagtttaa ttctgttttt tcacggaagg tcactatggc
       61 ttcgacaaat gcgcatcaaa taaaaaataa tgatcaaaac tctttatgtg gtctgggtga
      121 caaaatacgc cgcctgaccg ccggagtttg tttatttaca caaatttttt tccccgtcat
      181 ggcgacggca caaaatgtgg tacatgctaa acctcagacg acagtttcat ctgccccccc
      241 tctaatagaa aataatactg tgccttacac gcttggtgcg ctggaatcag cgcaaagcgt
      301 tgctgatcgc ttcggtattt cactggagga gcttcgtcgt cttaatcagt tccgtacttt
      361 tgctcgcggc tttgataacg tgcgccaggg tgaagaactg gatgttccgg caacaacctc
      421 gcagaaaagc catgagcaac aaaatgccgt accgcctgcg aatggcgaaa acacgctgga
      481 gaatcaaata gccagcacct cgcagcgagt tggcactctg ctttcacaag atatgaatag
      541 tgagcaggcc agcggcatgg cgcgtggttg ggcgtcttca gaagcctcag gcgcgatgac
      601 tgattggtta aataactttg gtactgcgag aatctatctg ggtgtggatg aagattttag
      661 cctgaaaaat tcacaattcg acttcctgca tccgtggtat gacacacccg attatctgct
      721 cttcagccag catacccttc accgaacaga cgatcgtacc cagatcaaca ccggtttggg
      781 ctggcgtcat ttcacctcca gctggatgtc aggcatcaac ctgttttttg accacgacct
      841 gagccgctat cactcccgcg cagggcttgg cgcagaatac tggcgtgatt atctgaagtt
      901 gagcagcaac gcttatatcg gcctgaccgg ctggcgtagc gcaccagaat tggataacga
      961 cttcgaagcc cgcccggcca acggctggga tttacgcgcg gaaggctggt tacctgcctg
     1021 gccacaactg gggggaaaac tggtctatga acaatactat ggcgatgaag tggcgctgtt
     1081 tgacaagaac gatcgccaaa gtaatcccca tgctattacc gcgggcctca actatacccc
     1141 cttcccgctt ctgactctca gtgcggaaca gcgtcagggg aagcaaggtg aaaatgacac
     1201 acgttttgcc gttgatctga cctggcaacc cagcagttca atccagaaac agcttaaccc
     1261 ggacgaagtg gccggacggc gcagtctggc cggtagccgt tatgacctga ttgatcgcaa
     1321 caacaacatc gttctggaat atcgcaagaa agagctgatt cgcctgagtc tgctggatcc
     1381 ggtgaaaggg aagtctggag aaataaaacc gctggtttcc tcgctacaga ccaaatatgc
     1441 ccttaaaggc tataacatcg aagccgctgc gctggaagct gccggaggta aagtcagcac
     1501 gtctggaaaa gatatcacgg tcacgctgcc aggttaccgc ttcactaaca ccccagaaac
     1561 cgataataca tggtcgatag acgttaccgc ggaggatgta aaaggtaaca tgtcacggca
     1621 tgaacaaagc atggtagtta ttcaggctcc gacattaagc cagaaagatt ctctgttatc
     1681 cgtcaatccg ctaaccgtgg ctgcagataa aaaatcgacg accacattga ccgttactgc
     1741 gcacgattcc gacggaactc cggtgccggg gctggcgctg caaacccgca gtgaaggcgt
     1801 tcaggatatc accctgtctg actggacaga taacggtgat ggtagttaca tacagatgct
     1861 gaccgccgga acgacatcag gttcagtaac actgacgccg caaattaacg gtgagagtgc
     1921 ggtaaaagaa tccatcgtcg ttaatatcgt ccctattgtc tcatcccgcg accattcatc
     1981 aataacaatt gataacgtat cgtattatgc cggagacgac atcaaggtta gggtggaact
     2041 gaaagacgac agcaatcaac cggttgcata tcaaaaagag gaattggtaa aagctgttac
     2101 tgtcgaaaac agcaaacctg gagccacgat tgtctggcac gaagagcagc cgggcgttta
     2161 taccgcgaat tatccggccc ataagcaagg gacagcacta agggtacaac ttagcctcca
     2221 caactggaat gctccactgc aatcgcatat ttataacatt gaggcaaacc agaataaggc
     2281 tcgcgtcgcc acattatcag cgacaaataa tgacgtttac gccgataaaa agacatttaa
     2341 taccctcact atcaacgtca ctgatgagag tgataatccc ctgataaatc atcaggtcac
     2401 ctttaagaat gaaaaaggaa gcgcggaatt tgtcgaaccg ccgcagcaaa atacggatgg
     2461 atacggtgtt gccacaatca acatggtaag tcaggttgcg gaagaaaata cgattagcgc
     2521 cacgctgcca aatggtttct cacaacggat aattgcgaaa ttcgttagcg attcgagtac
     2581 gccaaaattc aaacaactgg ttgccgatcc agataccatt attgctggca acagccaggg
     2641 cagtactctg accgccatcg tcacagactt tcataacaac ccgttaaaag atatgaaagt
     2701 gaattttgtg gcacctggtg gctcgcaact ggacaacacg accgccacaa cagaccagtc
     2761 cggtattgtg cgggtgcacc tgaccagttc aaaagctggt agctattccg tcgatgcctc
     2821 gcttgaggtg gataaaaata ttcaccagtc ggtcacgatc accgtggtcc caaacaggga
     2881 acaatcggta atgaccttga atgccgggtc gggcagtgcg atcgctaaca atacaaatat
     2941 cgttaccctg actgccagtg tgaaagatgt ttatggacac ccgttgccgg atgaggatgt
     3001 gaaatttacc ttgccagcct ccatgaccgg gaacttcacg ctaagtagtg aaaccgcccg
     3061 caccgatgca aacggtgatg ccgtggtcac attgcgaggc acaaaagcgg gtgagtttac
     3121 agttacggcg acgctgacca gaaataatac cgttgcttat cagcaagtca cttttattgg
     3181 ggatacaaac agtgcgcagc tccagccgct gactgcctca ttaaattcca ttgttgcggg
     3241 taacagtacg gggagtaccc tgacggcaac gatcctggac gcttaccaaa atccgcttaa
     3301 agaccagttg gtcactttcc agagtaatga tgtcactcta agcggaacag aagtcaccac
     3361 caatacgctg ggtcaggcga cggtaacaat gaccagcaat attgccggac aacataacgt
     3421 cgtggtgagc cggaaagcgc aagcctccga taataaaacg tttagtttat cagtgctgcc
     3481 ggatgaaagt tcggcgaagg taataagtat aaccggagcc gaaaaaacga taacggtggg
     3541 cgaaaacatc acgctacgga tactcgtcca ggacgcgttt aacaatgtaa tcgcgggtca
     3601 acgcgtcaga ttaagtgcgc agccaacaac taacattacg ataggcgata cggcttacac
     3661 cgataataac ggttatgcgt acgttcacct tctcagcacc cagcctgggg tttatcaggt
     3721 gacggcaacg ctggacaata acagtagtag taaggttgac gtgaatgtgg caaatggcaa
     3781 actcgagtta acatcatcga agccagaaac cacggtccat aatagtgagg gtattacgct
     3841 gaccgcaacg gcgagaaatg cgcggggtga attgatgcca gggcaaatta tcacctttag
     3901 cgtaacgcct gaaggtgcaa cgctaagcaa tacaggggaa gtccttactg accagtcagg
     3961 tcaggccaaa gtgacgctga ccagtgacaa agtgaatgtc tataccgtta cggccacaat
     4021 gggcaaagat gttcccgttc agagccaggt aacggttgcg gttaaggcag atgctaaaac
     4081 ggcacatgtt gtgagcgtcg tggcttctcc tgacaccatc accgccgacg gcatcgatag
     4141 cagcaccatc acttcacgag tagaagatga ttacggattc ccggttgaag gtgtcgatgt
     4201 tagtcatggc ttagacacca aaggcagccc ggtagttaat attccaacta cgcgtaccga
     4261 tcagtccggg caagtcacgg cgacaataac cagtacattg gcagaaacct taacagtcaa
     4321 tgtgcaagtt cctggtacag ccaaccaatc cgcaaccatt acattggttg ccggcacggc
     4381 cgatgaaagt aagtcaattt tgaaatccga tgttgacact ctgaaggctg actaccagca
     4441 gagcgcaaaa cttacgctaa cattgcaaga caagtacggt aacccgatag tgacgtctga
     4501 tcatctggaa tttgtccagt caggcccctt cgtgaacttt ctcaagttga gcgatattga
     4561 ttacagccaa agaaattatg gcgagtacac cgtgactgtc actggcggaa aagagggaac
     4621 agcgaccctc attcccatgc tgaacggggt tcatcaggca aacttaagca tatcgctgaa
     4681 tctcatccaa tcgataaaag aaatgtccgg tcatgtcact gcaaacaacc ataccttctc
     4741 cacggctaaa ttcccgagcg aaggctttgc aggggcgtat tacacactca acaatgataa
     4801 ctttgaagcg ggtaaaaccg ttgatgatta tatgttttca agttcacagg gttgggtgtc
     4861 tgtcgatgct tcgggtaaag tttctttcgc aaatatcggc gatcaaacgt cagtcacaat
     4921 aagcgctgtt ccccgacaag gaggtacaac ctaccagacc ttaattaagc tgaaaggctg
     4981 gtgggtgaat aatggaaatc ataccaatat ctggctagct gccaatgcgc tctgtcatgc
     5041 taaaaatgat ggatataatc ttcctggcat cacacatttg acgtctggcg aaaacaaacg
     5101 cacgcaggga tcactgtatg gtgaatgggg gaacgttgga gcgttttcca gtaattcgca
     5161 atttacaccg ggagcttact ggacaagtga atctgatgat tacagtcggc actactatgt
     5221 gcagatgcta accggtatga ccggaagcga cgctgattcc agcccccaac tgaccgcctg
     5281 ccgtaaatca ctttaatttc agtatgttat taaaggatac ccttttacga gggtatcctt
     5341 tcagactgat aacaaacgct acattgccgg atgtgctgcg cttatcaggc ctatataacc
     5401 cctgcaatat attgaattta ttgtttttgt accccagccg gcataaacga ctactcaaga
     5461 aactcaacag caaccgttga tgtcagccag accccgtttt ccgcttgcca gaaagggatg
     5521 cctcgtttcg ccatttcctg tgctttgacg gttaagacaa ccggcgatcc gtgtcttgct
     5581 cccactttgc gtgccgttgc ttcatcagca gagagatgca cgtaatgacg atcccctgca
     5641 atcagcccct gtttttttat ctcgtcaagg aaacggcttg ccgtaccgtg gtagagaaac
     5701 tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg aagtcgaatg cccctgaacc
     5761 gcgcggatgc acctaccatc actggaataa ctaaaacgct ttttatcgct ggtggcgacc
     5821 acggtatcca gcaatgcacg ggttaatcgc ttgcccgctt tttgggcaca aagtatgagc
     5881 ttatcgatat tcgcccatcc ttcacggtcc agtacgatac caatcgcctc cggtttgtgt
     5941 cggaggacaa aacttaaaaa cttgctcgta tccgccagct ctttttcgtt gtactttgcc
     6001 atcgcgtttt cgtccctggg ttttctgttt ttatttacca atgtcattgt cactggcttt
     6061 atgaattttc tgcaactcaa tcctgcgcag aaccttttcc acacagcgta atgtttcccc
     6121 tatgcaaaaa acgcatagcc agaaacatac ttgcattttc cccataccca catggctgat
     6181 aaggtttttc agttccagga attttcctgg ctcaagcaac acacaaacac aaaaaaacaa
     6241 tggggattgt tatgacacaa caaggggatg ctgtagccgg ggagctggcg acagaaaaag
     6301 tgggcattaa agggtatctg gccttttttc tcaccataat ctttttttcc ggtgtttttt
     6361 ccgggacaga cagctggtgg cgcgtttttg atttttccgt tctgaacgga tcgtttgggc
     6421 aactgcctgg tgcgaacgga gccaccacct catttcgcgg cgcgggtggt gcgggggcta
     6481 aagatggctt tctctttgcg ctggagctgg caccgtcagt cattctctct ctggggatta
     6541 tttcaattac cgatggtctt ggtggattac gcgccgcgca gcagttaatg acacctgtgc
     6601 taaaaccgtt gttgggcatt ccagggatct gctcgctggc gctgatcgct aacttgcaaa
     6661 acactgatgc cgccgccggt atgaccaaag agctggcgca ggaaggtgaa attaccgaac
     6721 gcgataaagt gattttcgcc gcatatcaaa ccagcggtag cgccatcatc accaactact
     6781 tctcttccgg cgtcgcggtg ttcgcctttc tgggcacgtc cgtaatcgta cctttagccg
     6841 tcatcctggt gtttaaattt gtcggagcca acattttgcg cgtctggctt aacttcgaag
     6901 aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact caggtccgca aaaacgtcat
     6961 ggatatgttt atcgatggcg cgcgccgtgg ctttactatc gccacgacca acctgctgcc
     7021 aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa attaccggcc tgctcgactg
     7081 ggtggggcat atctgtgaac ccgtaatggc gctgtgggga ttacccggtg aagcggcaac
     7141 cgtcctttta gccgcattaa tgagtatggg cggcgcagtc ggtgttgccg ccagtctggc
     7201 aaccgctggc gcattaaccg gtcacgatgt caccgtttta ctgcccgcga tgtatttaat
     7261 gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca gccgaagtga atgccaaata
     7321 ttacccgcat atcattacgg tctgcgtgat taacgcattg ctgtcgatct gggttatgca
     7381 gcttattgtt taacaaggag ttaccatgat tgattatacc gcagccggtt ttaccctgct
     7441 gcagggagcg catttgtatg cgccggaaga tcggggaatt tgcgatgtcc tcgtcgctaa
     7501 cggcaaaatt atcgccgttg ccagcaatat cccttctgac attgtaccgg actgcacggt
     7561 tgtcgatctc agtgggcaga tcctctgccc aggttttatt gatcaacacg tccatttgat
     7621 tggcggtggc ggcgaagcag gtcccacgac gcgcacgccg gaagtggcgc taagtcgcct
     7681 gacagaagcg ggcgtcacgt cagtggttgg tctgctgggc accgactcta tctctcgcca
     7741 cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa gaaggcatca gcgcctggat
     7801 gctgaccggc gcttatcatg tcccttcccg cactattacg ggttccgtgg aaaaagacgt
     7861 ggcgattatc gatcgcgtga ttggcgtgaa atgcgccatc tctgatcacc gttctgccgc
     7921 accggacgtt tatcacctgg ctaatatggc ggcagaatcc cgcgttggcg gtttgctcgg
     7981 cggtaaacct ggcgttaccg tgttccacat gggcgacagc aaaaaggcgt tacagcctgt
     8041 ctatgacctg ctggaaaact gcgatgtgcc gatcagcaag ttgctgccga cccacgttaa
     8101 ccgcaacgta ccgttgtttg agcaggcgct ggagttcgcg cgcaaaggcg gcaccatcga
     8161 tatcaccagc agcattgacg aaccggtcgc ccctgccgaa ggtattgccc gcgccgttca
     8221 ggcgggtatt ccgctggcac gcgtcaccct cagctccgac ggcaacggta gccagccgtt
     8281 cttcgatgac gaagggaatt taacccatat cggtgttgca ggttttgaaa cgttgctgga
     8341 aaccgtgcag gtgctggtca aagactatga tttcagtatc agcgatgccc tgcgcccgct
     8401 caccagtagc gtagccggtt tccttaacct gagcgggaaa ggcgaaattc tgcctggcaa
     8461 tgacgcagac ttactggtca tgacgccaga attgcgcatt gagcaggtat atgctcgcgg
     8521 caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga acgtttgaaa cggcttaaac
     8581 ggttgtcagc cgccctaatg gacgtagaat gcccgcaagg gcggctgaca gagtaaaacg
     8641 taatggatga ctgtggtgcg gttttgcata atattgaaac caaatggctt tatgattttc
     8701 tgaccctgga aaaatgccgc aatttttccc aggcggcagt cagtcgtaac gtctcgcaac
     8761 cggcattcag ccgccgcatc cgcgcgctgg aacaggcgat tggcgttgaa ttgtttaacc
     8821 gccaggtgac gccgctgcaa ctctcagaac aagggaaaat cttccattcg cagatccgcc
     8881 atctgttgca acagttagaa agtaaccttg cagagctgcg tggcggcagc gattacgcgc
     8941 aacgtaaaat caagatagcc gctgcacact ctctttccct cggtctgtta ccgtccatta
     9001 tcagccagat gccgccgctc tttacctggg caattgaagc cattgatgtc gatgaagcgg
     9061 tcgataaact gcgtgaaggg caaagtgact gtattttttc ctttcacgac gaagatttgc
     9121 tggaagcgcc gtttgaccac attcgcttat ttgaatctca attgttccct gtctgcgcca
     9181 gtgacgaaca cggagaagca ctttttaacc tcgcgcagcc acactttccg ttactgaatt
     9241 acagccgcaa ctcctacatg gggcgattga ttaatcgcac cctgacgcgc cacagtgagt
     9301 taagtttcag cacctttttt gtctcttcga tgagcgagct tttaaagcag gttgccctcg
     9361 acggctgtgg gattgcctgg ctgccggagt atgccatcca acaagaaatt cgcagcgggc
     9421 agctcgttgt gctgaatcgg gacgaacagg tgatcccgat tcaggcctac gcataccgga
     9481 tgaatacccg catgaatccc gttgccgaac gcttctggcg tgaactgcgc gagctggaga
     9541 ttgtgcttag ctgacattct ccagcgtcgc actgcgccaa ccgccattat cccagaccag
     9601 atcaaacgtt agcggttcat cgtcgtggta cagcattatc tcaaggctga tcgccagcgt
     9661 gtggcagtac gcttctttca aattgacctg caacgccgca atccgtggct catgaaataa
     9721 cagtgcagaa cgcagctgac ggcaatacca gctctggaga tgtttgtcgc tcgggttatg
     9781 cacttgccac tcgggcatgc ctgaaacaag taatgacggt aatgccgaat gaggagaatt
     9841 aaaaagagtg aggagatcgc tgcataccga tttacgcagc aatgacgttt tattgcctga
     9901 aatttcagtt aaaacagcct gaagtgattg gcgcatcatt ttgcgcactc ctgacgttta
     9961 gcaacatatg cggcttgccc tgaacaccgg gcaccactgt attgatgctc attttccata
    10021 gccattccct tttgttcatt acatccatgt cagtctgaga caggcctgaa tcctgtcagc
    10081 aaagtataaa accttacttg cctatgaata tctactgact tttcaggaaa aaagtcatga
    10141 tttgataaaa atcatcaaac tcaaaaaaaa accgctagta tattctgaat attccgaata
    10201 gttacgtaga tgaaaa
//
LOCUS       DAZUGK010000162        10208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZUGK010000162 DAZUGK010000000
VERSION     DAZUGK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.163"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            46..621
                     /gene="ssuE"
                     /locus_tag="ACHY5W_003583"
     CDS             46..621
                     /gene="ssuE"
                     /locus_tag="ACHY5W_003583"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4448412.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALNTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            614..1573
                     /gene="ssuA"
                     /locus_tag="ACHY5W_003584"
     CDS             614..1573
                     /gene="ssuA"
                     /locus_tag="ACHY5W_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFS4448413.1"
                     /translation="MRKIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSLIQTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1570..2712
                     /gene="ssuD"
                     /locus_tag="ACHY5W_003585"
                     /pseudo
     CDS             1570..2712
                     /gene="ssuD"
                     /locus_tag="ACHY5W_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2726..3517
                     /gene="ssuC"
                     /locus_tag="ACHY5W_003586"
     CDS             2726..3517
                     /gene="ssuC"
                     /locus_tag="ACHY5W_003586"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFS4448414.1"
                     /translation="MATPVKKRLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3514..4281
                     /gene="ssuB"
                     /locus_tag="ACHY5W_003587"
     CDS             3514..4281
                     /gene="ssuB"
                     /locus_tag="ACHY5W_003587"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS4448415.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LDLKGQWRNAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4324..6936)
                     /gene="pepN"
                     /locus_tag="ACHY5W_003588"
     CDS             complement(4324..6936)
                     /gene="pepN"
                     /locus_tag="ACHY5W_003588"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFS4448416.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7202..8404
                     /gene="pncB"
                     /locus_tag="ACHY5W_003589"
     CDS             7202..8404
                     /gene="pncB"
                     /locus_tag="ACHY5W_003589"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4448417.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIEPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8574..9974
                     /gene="asnS"
                     /locus_tag="ACHY5W_003590"
     CDS             8574..9974
                     /gene="asnS"
                     /locus_tag="ACHY5W_003590"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4448418.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2287 a         2636 c         2761 g         2524 t
ORIGIN      
        1 tatcaatgtt aacaaaacaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc
       61 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
      121 aaactaaatg gcctggatgt agaggtttat cactggaatc tgcaaaactt cgccgcggaa
      181 gatctgcttt atgctcgttt cgatagtccg gcactcaata ccttcaccga acagttgcaa
      241 caggctgacg ggctgattgt cgccacgcct gtgtataaag ccgcttattc cggtgcgctg
      301 aaaaccctgc tcgacctgct gccagaacgt gcgttgcaag gcaaagtggt gctaccgctg
      361 gcgacgggcg gtacggtggc ccatctgctg gcggtcgatt atgcccttaa gccagtttta
      421 agtgcgctga aagcccagga gatcctgcac ggcgtgtttg ccgatgactc acaagtaatt
      481 gattaccatc acaaacccca gttcacgccg aatttacaaa cccgtcttga taccgcgcta
      541 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgatct tcattctctg
      601 cgaggtaatg cccatgcgta agatcattaa actggcgctg gtgggattgc ttagcgtctc
      661 tacgattgtg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      721 cagtattggt atggtattgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      781 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      841 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      901 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      961 agaaaacagc ctgatccaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
     1021 aggttccagt tcgcacaacc ttttactgcg cgcactacgc caggccgggc ttaagtttac
     1081 cgatatacaa cccacttacc tgacacccgc tgatgcccgc gccgcgttcc agcaaggtaa
     1141 cgttgacgcc tgggctatct gggatcccta ctactccgct gcattattac agggcggcgt
     1201 gcgggtgttg aaagacggca ccgatctcaa tcaaaccgga tcgttttatc tggcagctcg
     1261 cccctatgca gaaaaaaacg gcgcttttat tcagggtgta ctggcaacct ttagtgaggc
     1321 cgatgcgtta acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg
     1381 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1441 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1501 tctggtgccg aaaaaagtcg atattcgcca acgcatctgg cagcccactc aactggaagg
     1561 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat
     1621 ctgggaacgg aagaaggttc acgcccggtt gatcacggtt atctgcaaca aattgcgcaa
     1681 gcggcggatc gtcttggcta taccggtgtg ctgatcccaa cggggcgctc ctgcgaagat
     1741 gcgtggctgg ttgccgcatc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1801 ctgcgtccca gcgtaacctc acctaccgtt gccgcccgcc aggccgccac gcttgaccgt
     1861 ctctcaaatg gacgtgcgtt gtttaacctg gtcacaggca gcgatccaca agaactggca
     1921 ggcgacggcg tgttccttga tcatagtgag cgctacgaag cctcggcgga atttacccag
     1981 gtctggcggc gcttattgct gggggaaacc gtcgatttca acggtaaaca tattcatgtg
     2041 cggggagcca aactgctctt cccgccgatt caacagccgt atcctccgct ttactttggc
     2101 ggatcgtcag acgtcgccca ggagctggcg gcagaacagg ttgatctcta cctcacctgg
     2161 ggcgaaccgc cggaactggt taaagagaaa atcgaacaag tgcgggcgaa agctgccgcg
     2221 catggacgca aaattcgttt cggcattcgt ctgcatgtga ttgttcgtga aactaacgac
     2281 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac tatcgccaaa
     2341 gcacaagccg cattcgcccg cacggattcc gtggggcaac agcgaatggc agcgttacat
     2401 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2461 cgcggcggtg ccgggacggc gctggtgggc gatggtccta cagtcgctgc gcgaatcaac
     2521 gaatatgccg cgcttggcat cgacagcttt gtgctttcgg gcgatccgca tctggaagaa
     2581 gcgtatcggg tcggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2641 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcga atgattttat ccctcgcagg
     2701 tcgcgcaaag ctaaggaaca cgatgatggc aacgccagtg aagaagcggt tattgcgcgt
     2761 tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct cctcagttgg
     2821 ctggctgtcg acgcgtattt tgccttcacc ggaaggagtg gtgacggcgt tctggacgct
     2881 ctccgccagc ggcgaactgt ggcagcatct ggcgatcagc tcctggcggg cgctgattgg
     2941 cttttcaatt ggcggatcgc tggggttgat tttgggactg attagcgggc tatctcgctg
     3001 gggagaacgg ctgctggata cctcaattca gatgttgcgc aacgtgccgc atctggcgct
     3061 gatcccgctg gtgattttgt ggtttggcat tgatgaatcc gcaaaaatct ttctggtggc
     3121 gctcggtacg ctattcccca tttatatcaa cacctggcat ggcatccgta atatcgatcg
     3181 cgggttggtg gagatggcgc gtagctatgg attatccggc ataccgctgt ttatccatgt
     3241 gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg cgttttgcgt tgggcctgat
     3301 gtggctgacg ctgattgttg ccgaaaccat ttctgccaat tcaggcattg gttatctggc
     3361 gatgaatgcg cgggagtttt tgcaaacgga cgtggtggtg gtcgccatta ttctttacgc
     3421 cctgctaggc aaacttgctg acgtcagcgc gcagctgtta gagcgcctct ggttgcgctg
     3481 gaacccggct tatcatttga aggaggccac ggtatgaata ctgctcgtct gaaccagggc
     3541 acaccattgt tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt cctgaaccaa
     3601 ctggatttac atattcctgc aggtcagttt gtggcggtgg tgggccgtag cggtggtggc
     3661 aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc caaccgcagg cgatgtgtta
     3721 gcgggcacca caccgctggc tgagattcag gaagacacgc gaatgatgtt tcaggatgcg
     3781 cgtctgctac cgtggaaatc ggtgattgat aacgttgggt tagaccttaa aggtcagtgg
     3841 cgcaatgccg cgcgtcgggc gttggctgca gtaggactgg agaatcgcgc aggggaatgg
     3901 cctgccgcac tttcaggcgg gcagaaacag cgagtggcgc tggcaagagc gttgattcat
     3961 cgaccgggtt tattgttgct tgatgaaccg ctcggggcgc tggacgcctt aacgcgactc
     4021 gagatgcagg atttgattgt gtcactttgg caggagcacg gctttaccgt actgttggtg
     4081 acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat tgaagaggga
     4141 aaaattggtc tggatttgac ggtggatatc ccccgtccgc gccggttagg gtcggtgcga
     4201 ttagcggagc tggaagcgga agtgttgcaa cgggtaatgc agcgtggtga gtctgaaact
     4261 cgcctgagaa aacaggggta aaatccccga atggcggcgc tattgccgcc attcggttat
     4321 ttattaagcc agtgctttgg tgatcttctc gtacagatcg ccagagagat tttccagccc
     4381 tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat aacgtttcag
     4441 gcgaatcagc ggctcaatca ggcgcgaagc cacctgcggg ttacggctgt tgaggtcggt
     4501 aagcatttcc accaggaact ggtaaccgct gccatcttcg gcatggaacg ctgccgggtt
     4561 gctgcccgca aacgcaccaa tcaacgaacg aatacggttc gggttgctca tggtaaatga
     4621 gcgatgctgc aacagaccgc gcaccgtctc cagcacattc gccgccgggc tggtggcttg
     4681 caggataaac catttatcca tcaccagacc gtcctgatgc cacttgtcgt cgtactcctg
     4741 catcagcgcg tcacggcatg gcagctgtgc agcaaccgcc gccgaaagcg ccgccagcgc
     4801 atcggtcata ttgtttgctt catggtattg cttgctcacc agcacgtcag ccagatgcgt
     4861 ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga gtgcgttttg caatatcttc
     4921 atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatccgc
     4981 cagttcagtc gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc
     5041 gatgatatcg aacagttcag ccatttcatt gacagaaggc agcgtcagga tttctgccgc
     5101 cagcgccgga tcaatcttct catcgagcag caccgcgcgg aaagcgtcag ccacatgtac
     5161 cggcagagac agcggctgcc cttgctgatg acgggcgacg ttcagcttga tgtaggttgc
     5221 cagcaaactt tgcgccgcat cccaacggga gaaatcatta cgcgcatgac gcatcaggaa
     5281 ggtcagttgc tgatcgctcc acttatattc cagtttcact ggcgcagaga attcgcacag
     5341 cagcgcaggc accggctgga agtagacatt atcaaaaaca aaggtctgtt ccgcctgggt
     5401 gacgttcagc acggaattca ccggatgacc gcctttctgc aacgggatca ctttgccttc
     5461 gttgtcatac aattcgatgg caaacggaat atgcagcggc tgtttttctg cctgatccgg
     5521 cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc tcagtttccg gattgtagtc
     5581 gtctttgacg gtcacaaccg gcgtaccgga ctggctgtac caacggcgaa aatgggagag
     5641 atcaacattc gacgcatctt ccatcgcctg cacaaagtcg tcacaggtcg ctgcgctacc
     5701 atcatgacgc tcgaaataga gttgcatccc tttctggaag ttttcttcgc ccagcagggt
     5761 gtggatcatg cgaatcacct ccgcgccctt ctcgtaaacg gtcagggtgt agaagttgtt
     5821 catctcaatg accatatccg ggcggattgg gtgcgccatc gggctggcgt cttccgcaaa
     5881 ctgcaatccg cgcatggtgc gcacattatt gatacggttt accgcgcggg aaccaaggtc
     5941 agagctgaac tcctgatcgc ggaagacggt taaaccttct ttcaggctga gctggaacca
     6001 gtcgcggcag gtcactcggt taccggtcca gttatggaaa tattcatggc cgataacgcg
     6061 ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg cgggccagca catatttgga
     6121 gttaaagata ttcagaccct tattctccat tgcgcccata ttgaagaaat ccaccgcgac
     6181 gatcatatag atgtcgaggt catactccag gccaaagcgt tcttcatccc atttcatgga
     6241 gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat cgacgtacag
     6301 ctccagtgct acttctcgac cagaacgcgt ggtaaaggta tcgcgcagta catcaaagtc
     6361 gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc actgtaccca
     6421 atggcgtccg ttttccagtt cgccttgcgc aacgcggtta ccattggaaa gcaggaaggg
     6481 atatttgatt ttatcggcaa taattttggt ggtaaaacgc gccagcacgt ccgggcggtc
     6541 gagataatac gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa gcgcatcgcc
     6601 tgactgataa agcccttcca gcgccgtatt cgccgccggg cttatttcat taatgatctt
     6661 aagcgtaaaa cgctccggca aattactgat gaccagtgcg ccctcttctt ctttccaggc
     6721 ggtccacggc tcatcattaa tatgaacaga aaccagtttg aggtcttcgc cgttgagacg
     6781 aagcggagca tctgatgcac catgacggac agcctggctg accgcggtaa cgaccgtctt
     6841 ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg gcgcacgata
     6901 atcgtgacgg tatttggctt gtggctgttg agtcataaat aacctttagc atcttttata
     6961 gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat cttcatcttt
     7021 tcaggtacaa acgcctttat tgctacattt ttataacata caccgcgtaa tgccatcgac
     7081 cagaaaggtg gcatatggtg tgatcggggt tcaataaatt gcgaaacaag gtatactcca
     7141 gcagttcctg aagatgttta ttgtactaaa cgctcctgta cgaggacgct actgcgcacc
     7201 tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg gatacagatg cttataagtt
     7261 gcatatgcag caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg cggagtttcg
     7321 ttgccgaggt gacgatctgc tgggtattta tgccgatgct attcgtgaac aggttcaggc
     7381 gatgcagcac ctgcgcctgc aggatgatga atatcaatgg ctttctgccc tgcctttctt
     7441 taaggccgac tatcttaact ggctacgcga gttccgcttt aacccggaac aagtcaccgt
     7501 gtccaacgat aatggcaagc tggatattcg tttaagcggc ccgtggcgtg aagtcatcct
     7561 ctgggaagtt cctttgctgg cggttatcag tgaaatggta catcgctatc gctcaccgca
     7621 ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact tctcggcgtt
     7681 aaccgccggt cttgatatgt cgcgcttcca tctgatggat tttggcaccc gccgccgttt
     7741 ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct ggttcgtggg
     7801 caccagcaac tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa cacaggcaca
     7861 cgaatggttc caggcacatc agcaaatcag cccggatcta gccaacagcc agcgagctgc
     7921 acttgctgcc tggctggaag agtatcccga ccaacttggc attgcattaa ccgactgcat
     7981 cactatggat gctttcctgc gtgatttcgg tgtcgaattc gctagtcggt atcagggcct
     8041 gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa gccattgcac attatgaaaa
     8101 gctgggaatt gagccacaga gtaaaacgct ggttttctct gacaatctgg atttacgcaa
     8161 agcggttgag ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg gtattgggac
     8221 tcgcctgacc tgcgatatcc ctcaggtaaa acccctgaat attgtgatta agttagtaga
     8281 gtgtaacggt aaaccggtgg cgaaactttc tgacagccct ggcaaaacta tctgccacga
     8341 taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta aaaaagccag
     8401 ttaatatcat cagggagcta atcggctccc tttttttacc tttaattccg aaatctttcg
     8461 ctgcatttgc gaattctgct tgtctgattg cagatgccag gtaacatagg tatccccccc
     8521 atttacgggt ggacaagtgt ttattttttc cgactattaa cagagagaat attatgagcg
     8581 ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt tgacagcgaa gtcaccgtac
     8641 gcggatgggt acgtacccgc cgagattcaa aagctggcat ctccttcctc gccgtttatg
     8701 acggttcctg ctttgatcct gtacaggctg tcatcaataa ttctctgccc aattacaatg
     8761 aagacgtcct gcgtctgacc accggttgct cggtcattgt gacgggtaaa gtcgtggcgt
     8821 cgccgggcca ggggcaacaa tttgaaattc aggccagcaa ggttgaagtt gctggttggg
     8881 ttgaagatcc agacacttac ccgatggcgg caaaacgcca cagcattgag tatctgcgtg
     8941 aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc cgtcgcgcgc gttcgccata
     9001 cgctggcgca ggcgctgcat cgcttcttta acgagcaggg attcttctgg gtttcaacgc
     9061 cactgattac cgcatctgat accgaaggtg ctggcgaaat gttccgcgtt tctacgctgg
     9121 atctggaaaa cctgccgcgt aacgatcagg gcaaagtgga tttcgacaaa gacttctttg
     9181 gtaaagagtc tttcctgacc gtatctggcc agttgaacgg cgaaacctac gcttgcgcat
     9241 tgtccaaaat ttataccttc ggcccgactt tccgtgctga aaactccaac accagccgtc
     9301 acctggcgga attctggatg ctggaaccgg aagtggcgtt tgctaacctg aacgatatcg
     9361 cgggactggc tgaagccatg ctgaaatatg tcttcaaagc ggttctcgaa gaacgcgctg
     9421 acgacatgaa attcttcgct gaacgcgtag ataaagatgc cgtttcacgt ctggaacgct
     9481 tcattgaagc cgattttgcg caggtggatt acaccgacgc agtgaccatt ctcgaaaact
     9541 gcggcaggaa gtttgaaaac ccggtttact ggggcgttga tctctcttct gagcatgagc
     9601 gttatctggc ggaagaacac tttaaagcac cggtagtggt taaaaactat ccgaaagata
     9661 ttaaagcgtt ctatatgcgc cttaacgaag acggtaaaac cgttgcggct atggacgttc
     9721 tggctccggg catcggtgag attattggtg gttcccagcg tgaagaacgt ctggacgtgc
     9781 tggacgagcg tatgctggaa atgggcctga ataaagaaga ttactggtgg tatcgcgatc
     9841 tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct tggttttgaa cgtctgattg
     9901 cttacgtaac tggcgtgcaa aacgtacgtg atgtgattcc gttcccacgt actccgcgta
     9961 acgccagctt ctaatttagc gtcttcaaga gccagcgccc gctggctctt tttttatctt
    10021 ttctgtcaag ttatctgttt gttaagtcaa gcaatctatt tgcaaccccg ccataacgat
    10081 catcctgtta cggaatatta cattgcaaca tttacgcgca aaaaataatc cgcattctta
    10141 ttgcggatta gttttttctt agctgatagc acaattttca tactattttt tggcattctg
    10201 gatgtctg
//
LOCUS       DAZUGK010000163        10103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZUGK010000163 DAZUGK010000000
VERSION     DAZUGK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.164"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            195..467
                     /gene="ynjH"
                     /locus_tag="ACHY5W_003591"
     CDS             195..467
                     /gene="ynjH"
                     /locus_tag="ACHY5W_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFS4448419.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(433..840)
                     /gene="nudG"
                     /locus_tag="ACHY5W_003592"
     CDS             complement(433..840)
                     /gene="nudG"
                     /locus_tag="ACHY5W_003592"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFS4448420.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            927..1547
                     /gene="ynjF"
                     /locus_tag="ACHY5W_003593"
     CDS             927..1547
                     /gene="ynjF"
                     /locus_tag="ACHY5W_003593"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4448421.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="ACHY5W_003594"
     CDS             complement(1556..2863)
                     /gene="ynjE"
                     /locus_tag="ACHY5W_003594"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFS4448422.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALIEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVNDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(2930..3583)
                     /gene="ynjD"
                     /locus_tag="ACHY5W_003595"
     CDS             complement(2930..3583)
                     /gene="ynjD"
                     /locus_tag="ACHY5W_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4448423.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVMLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="ACHY5W_003596"
     CDS             complement(3583..5118)
                     /gene="ynjC"
                     /locus_tag="ACHY5W_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4448424.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDANQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLFPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLGWGPQRRQLWLWLPILLPALQLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQSAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="ACHY5W_003597"
     CDS             complement(5091..6257)
                     /gene="ynjB"
                     /locus_tag="ACHY5W_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4448425.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLAPDPAALKEASDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="ACHY5W_003598"
     CDS             complement(6267..6815)
                     /gene="ynjA"
                     /locus_tag="ACHY5W_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4448426.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWMERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="ACHY5W_003599"
     CDS             complement(6815..7522)
                     /gene="ydjZ"
                     /locus_tag="ACHY5W_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS4448427.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERIIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="ACHY5W_003600"
     CDS             complement(7537..8214)
                     /gene="ydjY"
                     /locus_tag="ACHY5W_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFS4448428.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENTAAKVEYDGLPN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="ACHY5W_003601"
     CDS             complement(8219..8929)
                     /gene="ydjX"
                     /locus_tag="ACHY5W_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS4448429.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="ACHY5W_003602"
     CDS             complement(9096..9902)
                     /gene="xthA"
                     /locus_tag="ACHY5W_003602"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4448430.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2459 a         2680 c         2627 g         2337 t
ORIGIN      
        1 tgtttatgtg atgtgctttt gcagttttcg actataccat caagaatgtg acggtaagca
       61 agaaaaaaat gacccaggaa agcaaattaa taacgagagt aatctcataa ccaaatgcga
      121 taactcagac gctaaagttt tgccacccgg ctaccgttat cagtagcaga gacaataacg
      181 ttaaggagtt ccctgtgagt cgagcattgt tcgccgtagt attagcattt ccattaattg
      241 cactggctaa cccgcattat cgtccggacg tggaggttaa cgtaccgcca gaagtgttca
      301 gttcaggcgg gcagtcagcg cagccctgta ctcagtgctg cgtttatcag gataaaaatt
      361 attctgaagg agcggtaatt aaagccgaag gaatactgct gcaatgccag cgtgatgaca
      421 aaacccttag cactaatccg ctggtctggc ggcgcgtaaa gccataaatg cctctaatag
      481 tggaatgtca gcaggggcca gcggatattg cagcgcctct tcaggtgagc accagaccag
      541 cgcctgatgt tcatgagcct gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag
      601 atggataatt cgccccgaaa cttctcgctg atggctggta acatactcac ccacagttgc
      661 ttcgatgccc agttcttcgc gtaactcacg caccagcgcc tgccgctgac tttcatccgg
      721 ctcgacttta ccaccggcaa actcccataa tcccgcctga tcgctctggg cggggcgttg
      781 cgcgagtaaa attttgccat cacgttcaat gatggcggca acaacttcaa tcattttcat
      841 aaattacaca cgggcagata agcgaaaaat gccataatat ccgcactgtt gctcaatcag
      901 aaaggcttta tatttaagga gtgcctgtgc tagaccgcca tcttcatccc cggattaaac
      961 cgttgttgca tcagtgcgtg cgggttcttg ataaaccggg cattacgcca gatggtttaa
     1021 cattagtggg atttgccatc ggcgtgctgg ctctgccgtt tctggcgctg ggctggtatc
     1081 tggcggcgct agtcgtcatt ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg
     1141 cgcggcgcag agggcttacc gacgcgggcg gctttctcga tatttctctc gattttcttt
     1201 tttacgcgct ggtgcctttt gggtttattc ttgctgcacc ggagcaaaat gcgctggcag
     1261 gcggctggct gctgtttgcg tttattggta cgggcagcag ttttctcgcc tttgctgcgc
     1321 tggcggcgaa acatcagatt gataatcccg gttatgcgca taaatcgttt tattatctgg
     1381 gcgggttaac cgaaggcagc gagacgattt tgctgtttgt tctgagttgc ctctttccga
     1441 cctgttttcc gtggtttgcg tgggtatttg gtgcgttgtg ctggatgacg acctttacgc
     1501 gggtgtggag tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt
     1561 tgctgctgtc cgggccgcgt tcaccggttg ctaccggatt ttttggatcg ctgctccatt
     1621 cgtaccagcc gccgtcgtaa acggagacgt tattccagcc cattgctcgt gcgtacataa
     1681 aggtttcgga cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga
     1741 tattccatgc tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt
     1801 tatggaaatc ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta
     1861 tttcaccttt tggtttaata tagctgtaac cgctggtcgt accgataaac tctggccacg
     1921 aacgaatgct caccagcgat gcatcctggc gatgcagcag tccacgcgcc tgttccatat
     1981 caagcattag ttgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt
     2041 tcggtggcgt tcctcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat
     2101 ccagcaggcg cacatcattc acgccggcat aaagcataat ctgcgccaca cgcgctgcag
     2161 cgtatacgtc acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca
     2221 gcatcgcttt tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt
     2281 tggtatcgat gtagtcagca ccgggaatat ggctgataag gtaaagctta ggagcgcccc
     2341 aggccgcttc aatgactttc cagtcaccgg caggtttcgc cgtaacctct ttaccttgtt
     2401 gcaggtcgtg cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac
     2461 gggaaggttc gattagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca
     2521 gtcgcgtttt gactgcgtcg acatctttgc cattaccgta tagcgccacc ggggcatcgg
     2581 ctttcaggtt atgttgcttg atccacgcgt ttagctgttc ggtgctcatt ttgtcaagcc
     2641 agctagcaga gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg
     2701 gccagccgtt ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgttgaa
     2761 gctggtcaag tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc
     2821 ctaaagccat tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg
     2881 tagttttgag tgcgccattc tgaactgacg cattgggaaa aactttgcgt tatcgcagtt
     2941 tgttgtaatt ttctgaccac tgagccatat ccagaacaga actatcagca ggaacatcct
     3001 ggagatcgtg cgttacctga acgacgggga tcgccagttc gcgaacttcg ctgaacaccc
     3061 actggcgaaa attatcgcgc agagccacat caagacggct gaatggctca tccagtagca
     3121 acgcttttgg ctgggcgaga agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag
     3181 acaaagtggc aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat
     3241 tcacggcatt acgtcgggca ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt
     3301 gcccgacgct gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct
     3361 gtgcggtggg tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa
     3421 actgtccggc cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagacg
     3481 gccccattaa catgacaatg tcacctttat ccaccgtaaa gttaacgttt gtcagcaagc
     3541 aactttctgg taaacgtagc gaaacatttt tcacgcagag cattagcgga gtccttgtct
     3601 gacataacct acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaacaa
     3661 cagttgccat aaagcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac
     3721 cgcctcagtg gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg
     3781 cgcaatactg actgaaaatc ccaccgcgaa ggcaatcagc gtagggcgta gcataagcgg
     3841 acatttcacg taaaagaaga ttttggcccg cgaccagccc agtgtttgcg caatcaaaat
     3901 taaccgtgaa tcaatgcgct gccaggccga ttgcaggata aacagcatcc acggcatcac
     3961 ccacagcaga tacccccaga ccaccgccgt ccagcttcca tccaggttca gccatagcgc
     4021 cagcgtgtac tggcccgcca ccagctgcag tgccggtaat aaaatgggca accatagcca
     4081 caactggcga cgctgtggcc cccatcccag ccacagcagt aacaggagca aagcgatgaa
     4141 tgtcgccacc agccccattg tcaggctgtt gatgagcgct tcactattga tcgtcgactg
     4201 atccgcgagg atcgccagca gaaccacaca cagcacaccg gtcaagggta aaaaactcgc
     4261 cagcgtaatg cctggaaata aaggcgtggc aggcttacga acgccatcta cgcggggaat
     4321 agtacgccgc caggtacacc atagcagata gcccagcaaa acgtaggcgg cgagtaacaa
     4381 catcagcagc aggctggcga gtgcgccttt cgtttgttga ttggcgtcgc cctgagttaa
     4441 ccactgccag ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac
     4501 atccacgacc gacagcgacc aggcaacaat cgccagcatc gccattgcca gcgcaggcgc
     4561 gacggagggc aacagcagcc agttcaggca ttgccagcgg ctgtagccca gcgaatccag
     4621 cacgatgact tgctgtaaaa gccgtttttc actcaatacc gctgctaaga tccacagcag
     4681 aaatgcgctt tctttcaccg ccagcgtgaa gcccagccca atacccaatt tgtccattgg
     4741 cggagtgaaa tacgggaaat agtcataaag cagccctccg tcagcaaaga gcagaagggc
     4801 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg tgcacatacg
     4861 ctgccatttc ggcccaggcc acagcgccac aatcaccagc agggcaatca acaacgcccc
     4921 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     4981 ggcaaataac gcctgccagt gtgtcaacga caaagcaggc gatatcagcg tgagagctgc
     5041 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5101 ccgtaacggt gtagccattc ttgttccagc gcatttaccc aacctgcgtg cggttcagcc
     5161 agtaccggcg gcagatcttg cggcattctt gattgcaatg tttcgcgctg cccatcaggc
     5221 agtttttgcg gatcgagaac agaaggatcg ccccagacag cgggatctgc tttacgcaat
     5281 tgcgcatcgg gtgagagcaa gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5341 ttggcaggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5401 taactgcttg caggcaaatc gccgctggcg attttttgct gcgcatgcgc ggggttaaag
     5461 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5521 gggaaatcct ttccttcgcg ccacaaatac ggatgcagcg catcaagata ttgccacaag
     5581 ggagcagtga cacgggcgaa agtcgcatcg tccgacgctt cttttaatgc ggcgggatcg
     5641 ggcgccagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5701 ggataggtaa ccgtgccggg attgacttta gcaaactcca gtaaggcttg cggcgtttgt
     5761 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5821 gattccgcac cttgtgtcgg cactgaaaaa tcttcccgca ccggcagctg tgtgtcgaca
     5881 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctcttttaag
     5941 gtgcggaagt tttcgccgtt cacccagagc agatccaccg agccgcccgt tttacgtccg
     6001 gctgcggctt cggtctgaat gcgcttcacc gcgtctgcgg catccgccag acgaacaatc
     6061 ttcaggttta tagcgtaatg ggttttcatc tcgccgctaa cccagtcgag atagcggtta
     6121 attgcggtat cgccgcccca ggcgttaaac cagacagttt gccctttggc ctcattttta
     6181 atttcttgcc agtcactggc atgtgttgcc agagaaaaca gcgataacaa tcccagcaac
     6241 cacccacaat ggcgcatcat ctctccttaa gaacgttttt ccggaatacg gcacagcccc
     6301 tgagcgggaa tgtccatggc agaattaaaa cgagccgaca gattttgcag gccgatcaat
     6361 gccgtcagtt cggtgagcgc ctgggcgtca aaatgcgcag ccagtcgggt gcgcagggca
     6421 tcatcgaccg ttggcggcgt gacgcttgcg gcttcggcgt actccagcgc cagccgttct
     6481 tcatcgctga agagcgggct ttgtcgccag tcagccactg ccagcagttt atcgctgctg
     6541 ccggtgcgct cggcgacttt catactggtg atatccacac aaaactcaca caggcacatt
     6601 tgcgcaatgc gcgcgctgac aagcgatcgt actaccggat cgagcggtga gcgtttgcgc
     6661 tccatccagc caacaaacat cgacaccaga taaaagataa acgggatccg cccccaccag
     6721 cgaatggggc ttagcacctc gccgtaatgg cgacgatgca gccacgcctg cggacgtaaa
     6781 atgaaaggaa ttttgctaag cggtggtaaa cccatcaggc attcctcttc tggcgctcaa
     6841 gccatatttt cttcgccata aaaatcacca cggtcagggc aaacagaata aacagtccgg
     6901 tgacaaacca gaaggtaccg cctgttaaca tgctgcccgc ccaggaataa acaatagtcg
     6961 ccggtaactg accaagcccg gtggcgataa aaaacgagcg aaaacggatt gaagtcagac
     7021 cggcagcata gctgattgga tcgaaaggga caaaaggcaa taaccgacag accaggatgg
     7081 tgtgtttgcc gtagcgagtg aaaaagccgt ccatactgtc aagtactgtt ttgccggtta
     7141 atttttccac cacttcgcgg cccatcactc tggcgataaa aaagcacagc gccgcgccgg
     7201 ccatcgaact ggtccacgac agcaagccgc cccagaacgc gccaaatagc gacgcattgg
     7261 caaaggtgat caaaaacgca ggtagcggtg cagcaatggc ctgtaaaatc atcaacaaga
     7321 acgagacaac tgccgccagt gcgccgtaag actgaataat gcgttctata ccctgttggt
     7381 ccacggcggc aaacgccgca acgctgcgat tgataaactc atggacgccg ggaagcagcg
     7441 cccatgccag cagcatagcg aacaacagga tgatgagggt tatacggtag taccagattt
     7501 ttcgcgattg catcttcatc atcctttatc ctggctttat tcggtgattt taaaggtaac
     7561 cgttgccagc gtgttatccg ccgggagaac cgaggcattg cctttgaatt tcacttcgcc
     7621 gcgtttttcc accgcaccat aagtgtatgt tgcattgctg acgatgccga ccgggcagct
     7681 atccagacac accaggcagc ccgttttctt ctcttctgct gccgttaaat taccgccaaa
     7741 gcgcatgtcc agtttcttgc cattactgtc aacaatcact tcatcaaagg aatacgcttt
     7801 tgccgcccct tgccagttga ccgaaatatc cagtttgctg cccgtgacat gagtcgtttc
     7861 tttattatcc atcgtcatgt tttcgcccgg cgtaccacct gcctctttca gggcttcata
     7921 aaatgctttc ggggtcgcat aacccataaa cagcgattta tgtccgttgg agccatcttt
     7981 aaacacaata ccgtgacgag tgtcgtcggt gaggaaacga ccattaattt gcaccagcat
     8041 ggttaccgta tggttattgg catcgacgcg cagcggttgg ctgttcggaa gtccgtcgta
     8101 ttccactttt gccgccgtat tctctttttg atcgcagccg ctaagcccag caacagccag
     8161 taagcagagt gcagtcagtc cttttttcca tgacactgaa taatgttgca acatcgttct
     8221 atccttcatt ttttgggtga gtaagtgggt tgcggcgcga agcagacaga tccacatgtt
     8281 tgtgtcgggc gtagagtttg gcgagctgga cgagaataaa cagcgccagg cccgccagac
     8341 agagttgtaa aataaagcgc agcgtaatgc cttcattggc gagatcgctt gccatcacgg
     8401 tataaattac gattccgggc agggtggtta atgccgaaat aagggtatag ggccagaaag
     8461 caatagcggt taatccgtaa gcgtaatttt gaatattgta cggaaacagc ggaattaagc
     8521 gggtcagaat aagaaaatcg atgccgttac gcgcaatgcc tttttcaatg gcctggaagg
     8581 tatggctatg gccaacgtat ttcagcagta aatcacgtcc catccagcgc gccagcagga
     8641 atgagcacga ggaggccagc gtggcggcaa ttaatgagag tagtgtccct aagaacgggc
     8701 caaaaactat tcctcccgcg atcaccaata tgcttcctgg taacagaaag agggtggcaa
     8761 tgatgaataa cagaatatag aggctataac cgaacaatcc gctctggcga atgagtgtct
     8821 gtaagtgggg taaatcggtg agcagatcga ataaaccgaa agcgtggata gcgtaaatga
     8881 ccagcgcaaa aataagacag gcaaaaagaa atttacgctc agcgttcatc gtggagtttc
     8941 ctatatcaaa cttgataata gagaagtggt cagacaacta aaaatgcagt gacggtataa
     9001 caaagggcgg caggcaacaa atcattactg acagtttgtt gatttttctc aaggttaatt
     9061 ctcctggccc agtttgagcc aggagagctg ctaaattagc ggcggaaggt cgcccagacg
     9121 ggggcgtgat cggacggttt ttccatgctg cgaatttcat agtcgatgcc ggtttctacg
     9181 caacattctg ccagcggttg gctggcgagc agcaggtcga tgcgcagacc gcggttatcg
     9241 tcaaaacctt ttgagcggta atcaaaccat gagaaacgat ctgctgtttg cggattcgca
     9301 tggcggaagg tatcgaccaa tccccagctc atcagtctct ccatccattc gcgttcctcc
     9361 ggaaggaaag aacattttcc ggtacgcagc cagcgcttac ggttctcttc gccaatgcca
     9421 atatccaggt cgccaggact gatattcata tcgcccataa tcagcaccgg attttcacgc
     9481 ttgagttcgg tttccaaata attttgcagg ttctgataaa actgcgcctt cgccgggaat
     9541 tttatcggat ggtcgcggct ttcaccctgc ggaaagtaac cgttgatcac ggtgacattg
     9601 cccagcggcg aggggatttc cgccataata atccgccgtt gcgcctcttc gtcgtcaccg
     9661 ggaaagccgc gacgcacggc aatcggcgtc tctttggtca gcagtgccac gccataatgg
     9721 cctttctgcc cgtgataaaa cacgttgtag ccgagcttcg ccacctcttc gagcggaaac
     9781 atatcgtcat gaacttttgt ctcctgcagg ccaatcacat ccggttggtg cttttcgacg
     9841 atggcttcaa gctggtgggg cctggcgcgc aggccgttga tattaaaaga gacaaatttc
     9901 atagtcgctg ccatttattc agataaagag tgcgtggatg gtagcagaat ttcgctttgc
     9961 ttaccgcctg ttgttaagtg atttgatcga tggttgttaa tgatgtcaac gatggcgcaa
    10021 aaaatgcccg cttttggtgc gagctgcgtc agaatggcgc agtaatttcc agtaaatttc
    10081 gtcaatgatc acaattccag aat
//
LOCUS       DAZUGK010000164        10051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZUGK010000164 DAZUGK010000000
VERSION     DAZUGK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.165"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..848
                     /locus_tag="ACHY5W_003603"
     CDS             <1..848
                     /locus_tag="ACHY5W_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFS4448431.1"
                     /translation="VFNALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITQQYGTPDL
                     CIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQNLRAPVPMTWA
                     FMMREAGFPAVRPIYELPLSEDVLDEVREEIRSLGSYIALNLEGSSQERTFSLSIAEN
                     LIVKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRSAAIIKDAYIAI
                     TPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKS
                     VLARI"
     gene            complement(1430..9333)
                     /locus_tag="ACHY5W_003604"
                     /pseudo
     CDS             complement(1430..9333)
                     /locus_tag="ACHY5W_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            9814..9987
                     /locus_tag="ACHY5W_003605"
                     /pseudo
     CDS             9814..9987
                     /locus_tag="ACHY5W_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219032.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2409 a         2400 c         2424 g         2818 t
ORIGIN      
        1 aagtctttaa tgcgctcaaa cttgaacaga ctaaattaac attagtcaca atgactatgc
       61 aggatcacgc aacattaaaa gaaataaaag atttagcgaa gaacataaca cagcaatacg
      121 gtacgccgga tctttgtata gaggcgatgc gtaaaaagaa cctgaaaacg atgttattta
      181 tcagtcagtt gaaagcaaaa acgaattttc aggttgttgg tttaaccatg aagtgctatt
      241 cccctgtgtg taagaacgcg tcccggatgg atcagaatct ccgggctccc gtacccatga
      301 catgggcatt tatgatgcgt gaggcgggtt ttccagcagt caggccaata tatgaattgc
      361 cactaagtga ggatgtactc gatgaggtgc gcgaggaaat acgatcgtta ggatcttaca
      421 ttgcgcttaa tttagaaggt agctcgcagg aacgtacatt ttcactatcg attgcagaaa
      481 atctaatagt aaaaattcaa agtgaaacag atataccaat agtgatcgtt catggaccaa
      541 aaggggaaga taaagccagg gtattagtgg attgttataa taatgtctac cgtttatcct
      601 tatcaccctc gattaaacgt tcagcagcaa tcataaaaga tgcttatatc gcaataactc
      661 ctgacacctc aatattacat atggcaagtg cctataatac tcccgttgtt gcaatttatg
      721 ctgattacaa aacgcgatgg cccgcaatgg ccgatgtttc ggagtcagtc gtcgttgggc
      781 aaaaaattga caatataagt ctggatgaat tcgcaaaggc attaaaaagt gttttggcga
      841 gaatatgaga cagtacttat gcatagtaat gccgatctgt tatgaacggt ggtgtcatca
      901 atactctgag gctggtcaat catcacagaa aaccagcctt tggatcaaag ctgtactcac
      961 cggatacgat catagtcaca ccgaacgcga atcagatgga actaaaacat tgtaatgtat
     1021 ttcaccatca ttaataagac aacctgaact taaatccccg ttaacaggca tacattatta
     1081 tgagtggtac tgatgtaatt tgtaatattg aaatatgaac tgatattgtt tgtacgtcct
     1141 gatatatctt cctccttgat gtacttagct aaacattaac gtaatttgac tacatagcaa
     1201 caatgatcgt tatcaaaata gatcatatac tttgtttccc gctggcgtgg aacaggtggt
     1261 tataccatgt gtgaaagcgt ttattaaaaa cgaaaggagg ctcgactatc ttcagactaa
     1321 cagggaggta tgggaaaatg caggaatatt aatggagcgt ttgacagcaa acgaatgtaa
     1381 aagcagagcg ccccgaatgg agcactctgt aagaaaatat agcgaaaaat tagctctttt
     1441 tataacatgc cacatgaaaa tattcagatg tttgaacaga gccatgcgcc ccgttttcca
     1501 gggaaacaat gtattgtcgg cctccacctg cggtattaga agaccaatat atttctgaat
     1561 aaaaatcagc gtccatataa tgccccatat ctccccactc accgaacaga gaccctattt
     1621 cgcgtcggtt ttctccactg cttaaaagat tcacgccagg aatcgcgtaa ccgttgccga
     1681 tctcgttatt acaataattt tcagcacgac ttaatggtaa gataatatta ttgttatctt
     1741 tccaccagcc cttcacccgt acttgcgttt ggtaaatagc gccacctgat cttggggttg
     1801 ctgtgatttc tacagtattg ctatcaccgt catttttgaa tgtcacttta ccagtagcat
     1861 caacgcctac ccaggaggca gtgcttgaaa acgcataatc agccgccgtt tttcctggtg
     1921 caaagttgtc attattcaac tgataatatg ccccggtaaa tccctgcgaa gggaatgaag
     1981 ctgccggtag gtttgcacca ttcactgata ctgtaccggt cattggtctg gttcccgcgc
     2041 taatgaattc tatcgtggta ttcagacccg cctggtgaac accattcaac acggggatca
     2101 gcgttgcgat accctctccg ccgcctgtaa cagtggcttt gtactcgccg gagaaatttt
     2161 tgctgtagtc aattgcacta acttgcacat agggcacgtt agtacctgat tgcacaaatt
     2221 ccagcccttc agaaacattg atcggatgac ctgatagatc gtgtagaaca agatatagtt
     2281 cagcactttc ggtaaagtcc cctttcagtg atgaccggtt gtttttaagg acggactgcg
     2341 aggcgtctgc cgggcctgcc accagcgtta tatctactgt ttgcgctcca ccatagttcg
     2401 tttctgcgct taccgtgacg ttccctgtca tcgctcctct cacgcttgtt gtcgcgagtc
     2461 cgttttgatc ggtcaccgct gttaatgacg tcaatgtggc agagccgctt tttaagacga
     2521 agtttacatt gacaccttcg accaggttcc cactgctatc ttcaacagta gcctttaacg
     2581 tgctgatatc actgttgttg gccgtgagag tcgacggatc agcgataaaa ctagcaacat
     2641 gtgctgtgct tgggttgcca gtaaccttca cggttgtctg tgtctgaata attacgccgc
     2701 tatggatcga tgctgtcacc acatatgtac cgactttatt gctggtcaga acgacttgtg
     2761 cctcacctga agtgttggtt gtcgccgttt ggctacttaa cgttgcacct tcgggcgtta
     2821 cgctaaaggt aacgagttca tggctcaatg gagccccgtt tgcatgagtt aaccttacag
     2881 tcagagttgc tcccgatggg tcattggcgc cgataagcgg acttgatgac gtgagtgtta
     2941 gtcttagatc gattaccacc aggtccttct cataaaagga gccattcgcc agggatgctt
     3001 tcacagtata cgaaccgtat cttccaggcg tcatggtgac ctcagctata ccctgcctat
     3061 tcgtagaaac cgtatcctga ctaatgacca tgttaaaact tgacggctct gcactgaatg
     3121 tcacaagttg gtcagcaact ggattgccaa actggtcgtc gacgtgtgct ttaaccgcta
     3181 tgggttctct gattattacc tgaccttcag gcatttccag actggtgatc gtcgcagaat
     3241 caacatctgc gttgacagtt aacattcgaa tggcggcttc ggtaggtgcg attgccaaat
     3301 tagctgtaac tactttcgtt ccagcaatgg tgcttgtcac aaggatttct gccttaccct
     3361 gagcatccgt ttcgacactg gtgttactca gcgtggtggc ggggccgcgg aaattgacct
     3421 tgataccttc tagcgggttg ccgtaagcgt cagtgacgct tgccgtcagg gtcgcgccct
     3481 cggttgtgct cgcggtgaat gagcttgatg atgcggtaaa accacccatt ttagcggtac
     3541 cagcatcggc aatcaaagtc accggcttcg tatcactgac accgtaactg ttcacgctaa
     3601 ctgtcacggg ataggtgccg gattttgtac cgctcagtgt aacttcagcc ttgccgttga
     3661 tgtctgtaat ggcggaaccg ccttgtccga gagtgaagct tgcgctcaca tctgccggta
     3721 gtgtgaatgt taccgcctca ttggctaacg ggttgccgtt tccatcagtc actgttgcct
     3781 gaagtttggt catcccaatg ttattggcga tgaaattgtc ctcggtaacg ttaagattaa
     3841 cctgcgcagt gagcgtatcc gcagtaaagt tcaccaccaa ctgctcactc ttaccgccag
     3901 ccattgatgc tgtcacggta tgagcgcctg cttttgtacc tttcagcgtg actttcgctt
     3961 tgccttcagc attagtaatc gctttaccgc catcgctcag cgtgaagttc gccctcacat
     4021 cttccgacag agtaaatgtt acctcagcgt tggctatcgc attgccttct gtatcagcga
     4081 ctgttgctgt tagtgtcgtg aaatcgttat tgtcggcaac caccggatcc ttcgaggctg
     4141 ccagcgtgat ttcagcattc gcgacgttcg gcacgtaggt cactgtttgt tgcatcgaat
     4201 cgccattatc gagggttgcc gtcacttgat aaaccccggc ttttgtagcg gtaaagcttg
     4261 cgtaaaaata gccgtagtag ttggtagtat atataccgtt attactgagg gtcacgcctt
     4321 cacttggtga aacgctgagg gttacctgat gctgggggac accgttgccg taattatcct
     4381 tcacctcaat atacagactg gttgtctcgc ctgcggagac tgtatcaaaa agtgcatgta
     4441 gcaaagtgag atgtgccgta gacgcatcag cattgacatt aattgatgtg ctaagcgtgg
     4501 agctaccatt ttccagactg gcctcaacgg tatccggtct cgctcctgat tctatcgagg
     4561 agcgggtatt ggtataagtg atggtagcct taccctgttc gttcgttacg gcttggccgc
     4621 cattcgtcat ttcggcagag tttgttctgc tggtgaagtt cacagtcaca cctttcaccg
     4681 gaaagccatt attatcaacg actgttgcgg taatgacgct gccagagctg ttctgcgggg
     4741 taccggcgat tatgctgtct gggacaggcg tcaactcgat aatttttgcc gccgctgtgt
     4801 cgccaataaa atgcacagtt ttgttgtcgc tggcaccatt attagccagt gatgcagtga
     4861 ccgtctgctc accaaaggca acgcctgcga gagtggcctg cgcgatgcca gactcattgg
     4921 tattacttac tcccggggtc agggtgagtc ctgaagaggc tgagctgaat gttaccggaa
     4981 gattattcac ctcattgtcg aactgatctt taaccgttgc agttagcgtt gcgttatcga
     5041 cgccattacc tgtgatctca tcttttgaca tctgcaggac aacctgagcc aaggttttgt
     5101 ccgccacaaa tgttaccggc tgcgaatcac tggtattgtt attacccagc gttgcggtaa
     5161 ccgtatgcgt gcccgctttt ttacctttga gcgtgacatg cgcttcccca ttggcctgag
     5221 tgatggcaat accgttattt ccaagggtaa agtttgccgc cacgtcctgt ggcatggtga
     5281 agtttaccgt tatccccgcc accggatgat tcgacggatc tttcaccatc gccgtaagct
     5341 gcacgctgtc actgttattc gtcaccacct gttgagcagg gatgttcagg gtgacctgcg
     5401 cgttgctggc atcgggcgca atattgactg tcgtcgtata gccattgccg ttaagcagtg
     5461 tggcttcgac tgtatgaaca cccaaaaccg tgcccttaag ggtaacttca gcaacaccac
     5521 tgtcgttagt attgcttgtg ctctgactaa gttgagtatc tgccggcttg gtactgaagg
     5581 taacctgtag atcttttact gcgttatcaa aaggatcttt cactgttgct gtcagagtcg
     5641 tctcatccac gccattccca atgatttccg ctttcgatgt ttgcagaacg acaaccgcgc
     5701 tgtccttatc cgccacgaat gtgactggct gcgcatcgct ggcattattg ttacccagcg
     5761 ttgcggttac cgtatgtgtg cccgcttttt tacctttgag ggtgatatgc gcttcgccat
     5821 tggcctgagt gatggcaata ccgttatttt cgagggtaaa gtttgccgca acgtcctgtg
     5881 gcatggtgaa gttcaccgtt attcccgcca caggatgatt cgacggatct ttcaccgttg
     5941 ccgtcagctg cacgctgtcg ctgttattgg tcaccacctg ttgagccggg atattcagcg
     6001 tgacctgcgc gttgctggta tcgggcacaa tattgacgat tttcgtatcg cttttaccgt
     6061 taagcagtat ggcttcggct gtatgaacac ccaaaacagt gcccttaagg gtaacttcag
     6121 caataccgtt ctcattagta ttacgcgcgt tcagactcag ttgcgtgtct gcgggggagg
     6181 tgcggaagac tactgacaga tttttaacca cgttatcaaa aggatctttc actgttgctg
     6241 tcagagttgt ctcatccacg ccattcccaa tgatttccgc tttcgatgtt tgcagaacga
     6301 caaccgctct gtctttatcc gccacaaagg tcattggctg tgtatcgctg acattgctgt
     6361 tagccagacg agccgtgatc atatgcgtgc ccgctaatgt gccggtcagg gaggcgattg
     6421 ctatcccgtt gctatccgtc atgccttttc cgctgttgct aatcgagaac cgacttgcga
     6481 cgtcatttgg cacagagaag gtgatttctt tatcttttag tgggttgcca tttttatcct
     6541 gcaaggttgc cgtcagctgt tgaggcgctg tgtcggtgac ggtgatctcg cctgggggca
     6601 cactgagggt cagggctgca gtactttgat caccgataaa aatcacctgt tgattagcct
     6661 gagaaccaga gctcacagag gccgtaaccg tataatcacc atttttcaaa ctggtcagcg
     6721 tagctgtggc gatcccgtcg tggctattca cttcggtttg gctcagtttc gctgctgctg
     6781 aattaacatt gaaggtgacc ttgacgtcat tgagcaggtt gccttttgca tcccggactg
     6841 tcgcggtcat tgtggcgctg tcattgccgt cagcgaccac ttcatttttc gacttctgca
     6901 gatcaacctg cgcggtactc gagtcgccag accattaaca tccgtttttg cggtgttttg
     6961 attgttgaag gaagttgccg atccgcttaa taccgcaaac gtgacggtat gatcattgat
     7021 tgggttgctt ccttcatcag cgacattgac cgagacggtg tttgctgcat tctcattggc
     7081 gagcacacca ttattgctgg cagataatgt cgcaattttc gctgactgcg ggttggcgtc
     7141 gatgataaat ccagcggtat gcaaatcttc attccagttt tgcattaaca gcttcgcagt
     7201 aagcccactg cctttggtat aggcggtata ggttgcctta tagacgccat ctgcggtttc
     7261 tttccagtct gtagtgacac caggtttcac gttgtcgatg ctgactgcgg tattcagttg
     7321 ctgtttttgc tccttaacag gtttgtcatt ttcatctctc agttctaccg tcacctcgat
     7381 aggattgccg gagagataac ggtccttatc aatcttaatt gacgagtgag tccgggatga
     7441 cgaaacagaa atgatattca ccacggcggg ggctttagcc gcatccacac tgttcagctg
     7501 tggcatcagc gtcagcgtgc cagacatcgc tcctgtggtc aggatctggg tatagcttcc
     7561 gtcaccatta tctttccagt cagaaagggt gatgtcctga acaccttcgt gacgcgtcga
     7621 gagcaccagc ccgataacag gattacctgc tgcatcatga gcaataaaag tcagtgtggc
     7681 gcttgaatgg gaatccgcgc tcaacgtctg ggtacttaac gataccgagg aatctttctg
     7741 gcttagcgta ggtgcctgaa cgactaccat gctctgttca cgattcgaaa aattgccttt
     7801 gacatcttca gcggtgactt caatcggcca ggtgttatcg gtttctggcg tactggtgaa
     7861 ccggtaaccc ggcagggtaa ccagaatatc tttacccgtt gtaaccactt taccaccggc
     7921 agcttccaga gcggtggctt cgacgttata gcctttcagg gcatatttgg tttgtagcga
     7981 cgaaaccagt gatttcactt ctcctgactt ccctgtcacg gggtctgtca gggtcaggcg
     8041 aaccagttct tttttgcgat attccagaac gatgttgttg ttgcgatcca ccagatcata
     8101 acggctgcct gcaaggctac gccgtgcatc gacttcattc gggtcaagct gtttctgcat
     8161 cgcgctgcca ggttgccagg taaaatcgac ggcaaaacgg gtgtcatttt cgccctgttt
     8221 accctggcgt tgctccgcgc tgaaggtcat cagcgggaag ggggtatagt taagtccagc
     8281 ggttatggca tgaggattac tttgccgatc atctttatcg aacaggacca cttcatcgcc
     8341 ataatactgt tcatagacca gtttaccgcc aaggtgcggc caggcgggta accagccttc
     8401 tgcgcgtaca tcccagccat tggccgggcg tgcttcataa tcgttgtcca gttcaggtgc
     8461 gctgcgccag ttggtcagtc gcaaatagcc gttactgctt aatttaagat agtcacgcca
     8521 gtactccgcg ccaatgccgg cgcgggagtg gtaacggcta aggtcgtggt cgaaaaagaa
     8581 gttgatgccc gacatccatg tgggagtgaa atgacgccaa cctaagccgt tgttaatctg
     8641 cgtacgctcg tcagtacgat ggagagtatg ctgactgaaa aagagattat caggcgtttc
     8701 ataccacgga tggagaaaat cgaactggga gttcttcagg ctaaaatctt catccacgcc
     8761 cagcgtgatt cttgcggtac tgaagcggct taaccagtct gtcattgcgc ctgaagcctg
     8821 agaagaggcc catccacgcg ccatatttgc cgcttgctcg ctgttcatat cctcggcgag
     8881 cagagacccg atttgctgtg aagtactggc tatttgttgc tcaaggttgc cactgctatt
     8941 acccggtggc ggggttaaat tattttcact aacttgtgcc gggacatcca gttcatcacc
     9001 ctggcggaca ttatcaaaac ctcgagcaaa cgtacgaaac tggttgagtt tgcgtaactc
     9061 agccaccgaa ataccgaaac gttcggcaac gctttgggcc gattccagcg ctccaagggt
     9121 gtagggcacc gtattggcat ttgcaatggc aatttgtgca ggaactggtt gttgggttgc
     9181 ggcgtttacc acaccttgtg ctgccgcagc catagggaac acaagttgag ttatcaggca
     9241 gatacccgca gttaagcggc gtagtttaat tcccatcccg cataagattt gtcggtcatt
     9301 tatctcttct ccacttctct tcttcgtagc catgcttacc ttccctgaac gagctaacat
     9361 aaatgcgccc tttctagcac agcgaaaaaa tgtaaatcct gatagcaata tgattgaaaa
     9421 ttaataactt ttcgttaggc agttttgggt gtgagttgca agaggggaga ctactgaata
     9481 actcaagttt tataatcgag gggaaaatgg tgatggcgtt catagcaaaa cgccctcaac
     9541 cataaaggtc gagggcgctt aagatgttaa aaacccgcta tccgttaaaa aacaatgttc
     9601 aactaaggtc agtgacattg cggtaaaaaa gcgaattttt agaatttggc tcctctgact
     9661 gggatcgccg tagccattaa ctggctgatt ggtaagctaa aacaaaaaat tgagattgtc
     9721 aagaccacca gactgaccac cttttgtttg actgataaat gctaaatcaa aattttcccg
     9781 gtgatcggag ttatggagca aagaaaccat cggaaataga tgagctatca ggaaagaaac
     9841 cgtgttcgga ctgcttatat tcactaggct gaatatctgg aggaacggag cgagatgaag
     9901 ccgtggtggg cggattatct tgatgcaaac aacgacgggt gtgtttcgac ttaccagtat
     9961 gcccgccgcc tgaaacagac gtcgtgatac aattgactat ctgtgctcag acttattatt
    10021 agtgccaatg atacatgatt cctttaccgc t
//
LOCUS       DAZUGK010000165         9991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZUGK010000165 DAZUGK010000000
VERSION     DAZUGK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.166"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            166..318
                     /gene="hokA"
                     /locus_tag="ACHY5W_003606"
     CDS             166..318
                     /gene="hokA"
                     /locus_tag="ACHY5W_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFS4448432.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(295..366)
                     /locus_tag="ACHY5W_003607"
     CDS             complement(295..366)
                     /locus_tag="ACHY5W_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448433.1"
                     /translation="MRNAVRKAGITSRRRPLLFQIAG"
     gene            complement(506..718)
                     /gene="cspA"
                     /locus_tag="ACHY5W_003608"
     CDS             complement(506..718)
                     /gene="cspA"
                     /locus_tag="ACHY5W_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFS4448434.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(999..1289)
                     /gene="yiaG"
                     /locus_tag="ACHY5W_003609"
     CDS             complement(999..1289)
                     /gene="yiaG"
                     /locus_tag="ACHY5W_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFS4448435.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1723..2433
                     /gene="yiaF"
                     /locus_tag="ACHY5W_003610"
     CDS             1723..2433
                     /gene="yiaF"
                     /locus_tag="ACHY5W_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFS4448436.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2483..3457)
                     /gene="ghrB"
                     /locus_tag="ACHY5W_003611"
                     /pseudo
     CDS             complement(2483..3457)
                     /gene="ghrB"
                     /locus_tag="ACHY5W_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
     gene            complement(3561..4220)
                     /gene="yiaD"
                     /locus_tag="ACHY5W_003612"
     CDS             complement(3561..4220)
                     /gene="yiaD"
                     /locus_tag="ACHY5W_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFS4448437.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4373..6706
                     /gene="bisC"
                     /locus_tag="ACHY5W_003613"
     CDS             4373..6706
                     /gene="bisC"
                     /locus_tag="ACHY5W_003613"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFS4448438.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPVL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHLLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6675..7115)
                     /gene="yiaC"
                     /locus_tag="ACHY5W_003614"
     CDS             complement(6675..7115)
                     /gene="yiaC"
                     /locus_tag="ACHY5W_003614"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS4448439.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7112..7675)
                     /gene="tag"
                     /locus_tag="ACHY5W_003615"
     CDS             complement(7112..7675)
                     /gene="tag"
                     /locus_tag="ACHY5W_003615"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4448440.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7833..8531
                     /gene="yhjY"
                     /locus_tag="ACHY5W_003616"
     CDS             7833..8531
                     /gene="yhjY"
                     /locus_tag="ACHY5W_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS4448441.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8760..9962
                     /gene="yhjX"
                     /locus_tag="ACHY5W_003617"
     CDS             8760..9962
                     /gene="yhjX"
                     /locus_tag="ACHY5W_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4448442.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICSSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2372 a         2429 c         2576 g         2614 t
ORIGIN      
        1 tagaatagcc acgggtgctt gagactgttt gtctcaggta ttcaccgaaa ggcagacaga
       61 gaaaagcccc acctgactat aaatcaaagt gtgtactgcc cctctatgca tagacaccat
      121 aaggttagcc tcttactcgt tggaaatcaa catagggggc tgggaatgcc gcagaaatat
      181 agattgcttt ctttaatagt gatttgtttc acgcttttat ttttcacctg gatgataaga
      241 gattcactgt gtgaattgca tattaaacag gggagttatg agctggcggc atttttagcc
      301 tgcaatttga aagagtaagg gtcttcggcg ggaagttatt cccgcctttc ttacggcgtt
      361 gcgcattctc attgcaccca aatttattct tcacaaaaat aatactagat tttattacgc
      421 gatcgattat ttatttcctg aaaaacaaat aaaaaaatcc ccgccaaatg gcagggatct
      481 tagattctgt gcttttaagc agagattaca ggctggttac gttaccagct gccgggcctt
      541 tagcgccgct ttcgatggtg aaggacactt tctgaccttc gtccagagat ttgtaaccat
      601 cgttctggat agcagagaag tgtacgaaca cgtctttaga gccatcgtca ggagtgatga
      661 agccgaagcc tttgtcagcg ttgaaccatt ttacgatacc agtcatttta ccggacatag
      721 tgtattacct ttaataatta agtgtgcctt tcggcgatat ggcgtgcttt acagattttg
      781 aagcgttaaa ggaatgtgca ctacgagggg tatcaacgat aactcttgaa gggacttgcc
      841 ttactacact gctttaatgg tctgtacgtc aaaccgttga tgtgcattaa gccacgcatt
      901 ggcgggtgat gcaacaatta tttttcatat ttatgattaa tcggttggaa aacggtcctg
      961 tcatcaggac cgtaaacagc aataaagtgg ataaaagtct attccatcaa ctgcttactt
     1021 aatgccgggt tggcttgaat caaacgcatc aattttagtt cggcacttga aggcttcacg
     1081 cgtctggatt cccattcctt taccatggcg actgatacgc ccaaaacccg ggcgaaatca
     1141 tcgattttta atcctgtccc ttttcgtaac tgctcaattt cggtacagga cgttgttctg
     1201 tgcgtcaggg taattttctg cgtttcatct ttaaaaacaa tctgttccag gctgctcaac
     1261 agctcatgca ttggatcttt atattccatt gaaaactcct caaatcacac tgcgggatcg
     1321 tgaattgcat cgaagctcat taagaatagt cgggaaaagc agacacagcg gggtgaagtc
     1381 agggcatgct ctggctatga ttcgattcag ccagtcattt agcgtgactt atagccacct
     1441 aacgatctga taaatgccga tatatctcta accactcctg gtttgtaaag atttcgaaaa
     1501 tctaaccttc tgcgaaggcg taaatctctg gctacaaaaa aggcaacggc aatatcattt
     1561 tttaacaatg aaacaacaaa ttaaccttgt ttcattttct ccatgatttt accaggaaga
     1621 ttacgtagaa agggtatcct gcaagcctgt cctggactat ccttgtcacg tcagacacgc
     1681 gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc
     1741 tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg tagttagcct gagtgggtgt
     1801 tttgataaag aaggcgatca gcgtaaagcg tttatcgact tcctgcagaa tacagtgatg
     1861 cgtagcggtg aacgtctacc aaccctgact gccgatcaga aaaaacagtt tggtcctttt
     1921 gtctctgatt acgcgattct gtatggttat tctcagcagg tgaatcaggc gatggattcc
     1981 ggtctgcgcc cggttgtaga cagcgttaac gcaattcgcg taccgcagga ttacgttacg
     2041 caaagcggtc cgctgcgtga aatgaatggt tcgctgggtg tgctggcgca acaactgcaa
     2101 aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa aacagagtga tgacctgaaa
     2161 ccggtctttg atcaggcatt caccaaagtg gtaacgacgc cagctgatgc attgcaaccg
     2221 ttgattccgg cggcacaaac tttcacgcaa caactagtga tggttggaga ctacattgcc
     2281 caacaaggta ctcaggtgag ctttgtagca aatggcatcc agttcccgac ttcacagcag
     2341 gcaagtgaat ataacaaact gattgcgcca ttaccggcac aacatcaggc gtttaatcag
     2401 gcctggacta cagcagttac tgcaacccaa taaagagtaa aagcccgagc gattatcggg
     2461 ctttactcca cgcagtcgcg gcttagtccg cgacgtgcgg attcacacag ttcttctcaa
     2521 cctttccttg taacgcatca atcaaattat ccacggcaca ggcggccatg ccataacgcg
     2581 tctcatgggt ggcagatcca atatgcggta ctgcgacgac gttggccatt gagagcaacg
     2641 gcgaatctac ggacagtggc tcttgttcga agacatccag cccggcggcg tgaatttccc
     2701 ctttctgcaa tgctgcgatc agtgcatttt cgtcaaccac cgggccacgt ccggcattaa
     2761 tgaaaatggc ggaggatttc attttggcga attgttctgc gccaaacaga tgatgcgtct
     2821 catcagttaa cggcaggatc aggcaaacga aatctgactc ttgtaacagt gtatccaaat
     2881 cgcagtagcg ggcgttgaag cgttcttctg cttctttatg gtggcggcgc gcgttataga
     2941 ggatgggcat gttgaagcca aagtgcgcac gttgtgccag cgccataccg atccgtccca
     3001 tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc
     3061 tcgcggtcca ttcgcctgct tttacccgtt ctgctacctc cacaacccga cgagcggtag
     3121 acaacaccag cgccatcagc gtatcggcga cggtttctgt taatacggtt ggcgtgtgca
     3181 tcagcagaat ttttcgggcg gtaagcgcat cgacatcaaa attgtcatag ccgacggaga
     3241 tcgttgatgt ggcacgcagt ttcggcattt tttccagcaa tgcggcatca acattctcgt
     3301 ttgaacccag taaaccttca gcttcggcaa aaattgctgc attttgttcg acggtttgtg
     3361 ggctgaggtt tgccacctag tgaacggtga aatgctcttg caggcgttgc agtaaatcat
     3421 caggtaaggc tttgtagagg ataacggacg gcttcatgct tctctccatt cactgataag
     3481 gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa gaatgccagg tgatttctgc
     3541 atcacctggc atgaaaggga ttacagcggg cttaaggtaa tttctacacg gcggttttgc
     3601 gccttacctt ctgcggtgct gttgctggcg attgggttag ccgggccaag gccctgagta
     3661 cggatgcggc tggcgtccac gccctgggtg atcaacgcgc tggcaacgga atccgcacgc
     3721 tgctgggaga gacgcatgtt cagatcgtga ccacccgtgc tgtcagtata accaatcacg
     3781 ttaaccgccg ttttcggata ctctttcagt accattgcca cgccggtcag ggtgttagcg
     3841 cccgccggtt tcagggtcgc gctgctgctg tcgaaggtca cattgttcgg catattgagg
     3901 ataatgttat ccccgctgcg ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc
     3961 ttcgcttcct gcacatccat gtaataaccc acgccgccgc ccagagctgc gcctgctgct
     4021 gcgccaatca gcgcgccttt accgcgatct ttcttcgaag aagagagcgc accaataccc
     4081 gcgcccacga gagagcccag acctgcgccg atagcagatt tacctgcttc gcgttcgccg
     4141 gtgtaagggt tagttgtgca gccagatacc gccagagcgc cactcactac ggcggcaata
     4201 agataaacac gtttcttcat tgttaatcct taataacctt tttattcttt gccacgggtt
     4261 ccgtggcggg agattatgcc gcgtgaacat gaagattatt cctgggaata ctcggaaatt
     4321 tgtaagtaat atttaactgc tcaatacatc taacctttca ggagtcttcg gtttggccaa
     4381 ctcatcctca cgatattccg ttctgactgc cgcccactgg gggcccatgc tggttgaaac
     4441 cgacggtgaa accgtgttta gctcgcgtgg cgcgttagcc acaggaatgg aaaactcctt
     4501 gcagagcgcg gttcgcgacc aggttcacag caatacgcgg gtacgatttc caatggtgcg
     4561 caaaggcttt ctcgcgtcac cggaaaaccc gcaaggcatt cgtgggcagg atgaatttgt
     4621 tcgcgtgagt tgggatgagg cgctggatct tattcaccaa caacataaac gcattcgtga
     4681 ggcttatggt ccggcatcga tttttgctgg ttcctacggc tggcgttcaa acggcgtgct
     4741 gcataaggcc tcgacattat tacaacgcta tatggcgctg gcaggcggtt ataccgggca
     4801 tctgggggat tattcgaccg gcgcggcaca ggcgatcatg ccgtatgtcg tgggtggtag
     4861 tgaagtttat caacagcaga ccagttggcc gctggtgctg gaacatagcg atgtcgtggt
     4921 gctgtggagt gctaacccac tcaatacgct gaaaattgcg tggaatgcat ccgatgagca
     4981 ggggctttct tacttttctg cactgcgtga cagcgggaaa aagctgatct gcattgatcc
     5041 aatgcgatcg gaaaccgtcg atttctttgg cgataaaatg gagtgggtgg caccgcacat
     5101 gggcaccgat gttgcgctga tgctggggat cgcccatacg ctggtggaaa atggttggca
     5161 cgacgaagcg tttctggcgc gttgcaccac aggttatgcc gtcttcgcct cttatttgct
     5221 gggcgagagt gacggaatag cgaaaaacgc cgaatgggcg gcagagattt gtggtgttgg
     5281 cgcagcgaaa atccgcgagc tggcggctat tttccaccaa aataccacca tgctgatggc
     5341 aggctgggga atgcagcgcc aacagtttgg tgagcaaaaa cactggatga tcgtcacgct
     5401 ggcagcaatg ttggggcaaa tcggcacacc cggcggcggt tttggtcttt cttaccattt
     5461 tgccaatggt ggtaacccca cgcggcgcgc tgcggtactc tcttccatgc agggcagctt
     5521 gccgggtggc tgcgatgcgg tagataaaat ccctgtcgcc cgcattgttg aagcactgga
     5581 aaaccccggt ggcgcatatc aacacaacgg tatggaccga catttccctg atattcgttt
     5641 tatctggtgg gcgggcggtg ccaactttac tcatcatcag gataccaatc gcctgatccg
     5701 tgcctggcaa aaaccggtgc tggtggtgat ctctgaatgc ttctggacgg cggcggcgaa
     5761 acacgcggat atcgttctgc ctgcgactac ctcttttgag cgtaatgatc tcaccatgac
     5821 cggtgattac agtaatcagc atctggtgcc gatgaagcaa gtggtgccgc cacgctatga
     5881 agcgcgtaat gattttgatg tttttgccga gttaagtgaa cgctgggaga agggcggtta
     5941 tgcacgtttt acggaaggaa aaagtgagct gcaatggctg gaaacgtttt ataacgttgc
     6001 ccggcagcgc ggggcaagcc agcaggttga attgccgcca tttactgagt tctggcaagc
     6061 caatcagtta attgagatgc cggaaaaccc ggacagcgag cggtttattc gcttcgctga
     6121 tttttgccgc gatccgcagg cgcatctatt aaaaaccgcc agcggtaaga ttgaaatctt
     6181 ctcgcagcgt attgccgatt acgcttatcc ggattgcccg gggcatccaa tgtggctggt
     6241 gccggacgaa tggcagggca atgccgaacc ggaacagtta caggtacttt ctgctcatcc
     6301 ggcgcatcgt ctgcacagcc agctgaatta cagttctctg cgcgaattgt acgcggtggc
     6361 aaaccgtgag cctgtcacca ttcatcctga cgatgcccag gcgcgcggca tacaagatgg
     6421 cgatactgtt cggctgtgga actcacgcgg gcaaattctt gccggagcgg tcattagcga
     6481 gggaattaaa cctggcgtga tttgcattca cgaaggggca tggccggatc tggatttaac
     6541 cgctgacggt atttgtaaaa acggcgcagt gaacgtgctg accaaagatc tccccagctc
     6601 gcggctgggg aatggctgtg cgggtaatac ggcgctggca tggctagaaa aatacaacgg
     6661 tccggaactg acacttacag cgtttgaacc accggccagc tcataatcca ggtgggtagt
     6721 tgggtttcat cctgccatgc gcaatcgaca atgtgaaaac cctgcgcctg gtaaaaatct
     6781 atcgccggtt gatttttttg ataaacctcc agcatcagat ggggatggcg ctgttgcaca
     6841 tactgcatca gtgccttacc aataccgcgc ctgacggcct tcggtgcgac aaacatcgct
     6901 gccagaaatc ggccttccat aatgctgaca aaaccgagaa gcttactgtc ttcttcccag
     6961 acccagtttt gtgcgttggc aagataggca tcccgcacca gcggaatgca ctcgcgccag
     7021 taattcgctt ttataaaggg atggccccag gttgtacttt ccagccacag ttcgaggatc
     7081 gcggggagtt ctgaacgttg cgcttcccta atcatggttt atttcccgga tagcaacagc
     7141 agccaaccac atgatcattc accagcccac atgcctgcat aaaggagtaa cagattgtgg
     7201 taccgacaaa cttaaaacca cgttttttca gtgccttaga tagggcgtcg gaggcgggcg
     7261 tagatgtggg gatttcgctc aacgttgtgg cttgtgtcac ctgtggctga tgatttacaa
     7321 acgaccagac aaagtcggca aacggttcgc cgttctgttc catttgcagg tacgcccgcg
     7381 cattaccaat aattgcctga attttcccgc gatggcggat aatcccggcg tcctgtacca
     7441 gtctttcgac atcctcttcc tgcattgcgg caaccttcac cggatcgaac tgatgaaagc
     7501 aggcgcgata gttttcgcgt tttttgagga cggtgatcca cgataaccca gcctgctgcc
     7561 cttccaggca gatcatttcg aacagttttt tactgtcagt ttcaggcacg ccccactcat
     7621 tatcatggta ggcaatataa agcgggtcct gactcaccca gccgcaacgt tccatacttt
     7681 ccctcgctat gtacccgatc gtaaaaattt cagccgcttt tcttgacgca tcggtgaaaa
     7741 agtcaatatt taatacaggg ctactatgcc cgatatcctt catacaatga caataatatt
     7801 gccgaattcg gctcttccct ggagtcgtgt taatgatcat aaaaaaaagc ggtggtcgct
     7861 ggcagctaag cctgctggcg agtgtggtaa tcagtgcctt ttttctcaac acagcttacg
     7921 cctggcaaca agaatatatc gttgatacgc aacccgggca ttccacagag cgttacacct
     7981 gggatagtga tcatcaacct gattacaacg atattttgtc gcaacgtatt caaagtagcc
     8041 aaagggcgct gggactggaa gtcaatctgg cggaagaaac ccctgtggat gtgaccagca
     8101 gtatgagtat gggctggaat tttcctttat atgaacaggt tacaactggc ccggtcgcgg
     8161 cattacatta cgatggcaca accacctcga tgtataacga gtttggcgac agtactacca
     8221 cgctgaccga tccgttatgg catgccagcg tgagtacctt aggctggcgt gttgactccc
     8281 ggcttggcga tctccgaccc tgggcgcaaa tcagctataa ccagcaattt ggcgagaata
     8341 tctggaaggc gcaatcaggc ctgagccgga tgacggcgac aaaccagaac ggcaactggc
     8401 tggatgtcac cgtaggcgct gatatgttgc tcaatcaaaa tattgccgcc tatgccgcgc
     8461 tatctcaggc agaaaatacc actaacaata gcgattatct gtatacgatg ggggttagtg
     8521 ccagatttta acgtaacaat cacaattgaa accattgaat aacaatagtt gtggcgatag
     8581 tgggtgctaa cttaccaaat aataaatttt gtgaataatt gtcgcgtcat tcattcctga
     8641 actaaggcat ttcattccgt tctgatggca tttcatgccg tttttcccca ggcataaagt
     8701 gcacttcgtt atggttgtcg gcagagattt ttccttttta ttattgcagg aatactgcca
     8761 tgacaccttc aaattatcag cgtacccgct ggctgacact catcggtact atcattaccc
     8821 agtttgcgct ggggtcggtt tatacctgga gcctgtttaa cggcgcgctt tccgccaagc
     8881 tggatgcgcc ggtaagccag gtcgctttct ctttcggctt gttaagtctg gggctggcaa
     8941 tttcgtcttc tgttgcgggc aaattgcagg aacgttttgg tgttaaacgc gtcaccatcg
     9001 cttccggcat tttgctggga ttagggttct tcctgacagc gcattctgac aacctgatga
     9061 tgctgtggtt aagcgccggt gtgctggtcg gtttggcgga tggcgcgggt tacctgctga
     9121 cgctctctaa ctgcgtgaaa tggttcccgg agcgtaaagg tctgatctcc gcgttcgcta
     9181 tcggttctta tggtctgggt agcctgggtt tcaaatttat cgacacgcag ctgctggaaa
     9241 cggtcggtct ggaaaaaacc tttgtgattt ggggagcgat tgcgctggtg atgattgttt
     9301 tcggcgcaac gttaatgaaa gacgcaccaa aacaggaagt gaaaaccagt aatggcgtgg
     9361 tggagaagga ctacacactg gcagagtcta tgcgtaaacc gcaatactgg atgctggcgg
     9421 tgatgttcct tactgcctgc atgagcggtc tgtatgtgat tggtgtggcg aaagatattg
     9481 ctcagagcct ggcgcatctt gatgcaattt ccgcagccaa cgcagtaacg gttatttcta
     9541 ttgctaacct ttctggtcgt ctggtgctgg gcattctgtc tgacaaaatt gcccgtatcc
     9601 gcgtcatcac tattggacag gttatttcgc tggtaggtat ggcggcactg ctatttgcac
     9661 cattgaatgc agtgacgttc tttgcagcga ttgcctgcgt ggcatttaac tttggcggca
     9721 ctattaccgt ctttccgtca ctggtcagtg agttcttcgg cctcaataac ctggcgaaaa
     9781 actacggtgt gatttacctc ggtttcggta tcggtagcat ttgtagttcg attatcgcct
     9841 cactgtttgg cggcttctat gtgacgttct acgtcatctt tgctctgctg attctgtcat
     9901 tggcgctttc tacgacgatt cgtcagcccg agcagaaagt attgcgtgag gcgcatattt
     9961 agccagaaac tacagatttt gaacgaaagg g
//
LOCUS       DAZUGK010000166         9842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZUGK010000166 DAZUGK010000000
VERSION     DAZUGK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.167"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            296..1273
                     /gene="glaH"
                     /locus_tag="ACHY5W_003618"
     CDS             296..1273
                     /gene="glaH"
                     /locus_tag="ACHY5W_003618"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFS4448443.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1293..2561
                     /gene="lhgO"
                     /locus_tag="ACHY5W_003619"
     CDS             1293..2561
                     /gene="lhgO"
                     /locus_tag="ACHY5W_003619"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFS4448444.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCQAGNRVTKAFCDQNGIRYDNCGKMLVATSELEMERM
                     CALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEYNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNNLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2584..4032
                     /gene="gabD"
                     /locus_tag="ACHY5W_003620"
     CDS             2584..4032
                     /gene="gabD"
                     /locus_tag="ACHY5W_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4448445.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELASRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVNVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4046..5326
                     /gene="gabT"
                     /locus_tag="ACHY5W_003621"
     CDS             4046..5326
                     /gene="gabT"
                     /locus_tag="ACHY5W_003621"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFS4448446.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5563..6963
                     /gene="gabP"
                     /locus_tag="ACHY5W_003622"
     CDS             5563..6963
                     /gene="gabP"
                     /locus_tag="ACHY5W_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFS4448447.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6984..7646
                     /gene="csiR"
                     /locus_tag="ACHY5W_003623"
     CDS             6984..7646
                     /gene="csiR"
                     /locus_tag="ACHY5W_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFS4448448.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMNLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7647..8096)
                     /gene="kbp"
                     /locus_tag="ACHY5W_003624"
     CDS             complement(7647..8096)
                     /gene="kbp"
                     /locus_tag="ACHY5W_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFS4448449.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8180..8338)
                     /locus_tag="ACHY5W_003625"
     CDS             complement(8180..8338)
                     /locus_tag="ACHY5W_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS4448450.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8521..8820
                     /locus_tag="ACHY5W_003626"
     CDS             8521..8820
                     /locus_tag="ACHY5W_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4448451.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8830..9354
                     /gene="ygaP"
                     /locus_tag="ACHY5W_003627"
     CDS             8830..9354
                     /gene="ygaP"
                     /locus_tag="ACHY5W_003627"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFS4448452.1"
                     /translation="MALTTISPHDAQELIAHGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVTSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9401..9805)
                     /gene="stpA"
                     /locus_tag="ACHY5W_003628"
     CDS             complement(9401..9805)
                     /gene="stpA"
                     /locus_tag="ACHY5W_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFS4448453.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2244 a         2528 c         2706 g         2364 t
ORIGIN      
        1 tgtttagttt actgacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
       61 taatctgttc tctgatggta attcccccct aacctgttgc tttagttatt catttcctgt
      121 ctcactttgc cttaataccc tacgttaaat gttactaatt tgttgttttt gatcacaata
      181 agaaaacaat atgtcgcatt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
      241 gctacgaaat gagcatcgcc atgtcaccct acatatcata agaggatcgc ttctgatgaa
      301 tgcactgacc gccgtacaaa acaacgctgt cgattcaggc caggactata gcggattcac
      361 cctcacccca tcggcgcaat ccccgcgtct gctggaactt accttcaccg aacagacgac
      421 caaacagttt ctcgaacagg ttgccgagtg gcccgtgcag gcgctggagt acaaatcgtt
      481 tctgcgtttt cgggtaggca aaattcttga tgatctgtgt gcgaatcagc tgcaaccgtt
      541 gctgttgaag accctgctaa accgcgctga aggtgcgttg ttgatcaatg cggtgggggt
      601 cgatgatgtg aagcaagcgg atgagatggt gaagctggcg acggcggtgg cgcatctgat
      661 tggtcgctcc aatttcgacg caatgagcgg tcagtattac gcgcgttttg tagtgaaaaa
      721 tgtcgataac tcagacagct atctgcgcca accgcaccgc gtgatggagc tgcacaacga
      781 cggtacctac gttgaagaga tcaccgacta tgtgctgatg atgaaaatcg acgagcagaa
      841 catgcagggt ggaaattcgc tgctgctgca tctcgacgac tgggaacatc tggaccacta
      901 tttccgccac ccgctggcgc gtcgcccgat gcgctttgcc gcgccgccaa gcaaaaacgt
      961 cagcaaagat gttttccatc cggtgttcga cgttgatcag cagggtcgcc cggtgatgcg
     1021 ctatatcgac cagttcgtcc agccgaaaga cttcgaagaa ggcgtgtggt tgagcgagct
     1081 ttcggacgcc attgaaacca gtaaaggcat tctttctgta cccgttcccg ttggcaaatt
     1141 cctgttgatt aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     1201 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgcctcta accactacca
     1261 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     1321 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ctatccggat gcccgcattg
     1381 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     1441 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc caggcgggaa
     1501 accgcgtcac caaagccttt tgcgatcaaa acggcattcg ctacgacaac tgcggcaaga
     1561 tgctggtcgc cacgtctgaa ctcgaaatgg aacggatgtg cgccttatgg gaacgcacgg
     1621 cggcgaacgg tatcgagcgc gagtggctaa acgccgatga actgcgcgag cgcgaaccga
     1681 atatcaccgg gcttggcggt atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     1741 tcacggcggc gatggcaaaa atcttccagg ccagaggcgg cgagattatc tataacgccg
     1801 aagtcagcgg ccttagtgag cataaaaacg gcgtggtgat acgtacccgt cagggtggcg
     1861 aatacgaagc gtcaacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     1921 tgctcggcct cgaaccgggc tttattatct gcccgttccg tggcgagtat ttccgcctgg
     1981 cgccggagta taaccagatt gttaaccatc tgatttaccc cattcccgac cccgcaatgc
     2041 cgtttttggg cgttcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     2101 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agcgacacgc
     2161 tggagatttt aggctcgtcg gggattcgcc gggtgctgca aaacaatcta cgctcaggac
     2221 tgggcgagat gaaaaactcg ctgtgcaaga gcggctatct gcggctggtg caaaagtatt
     2281 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcgg
     2341 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccaccccg cgcacgatcc
     2401 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     2461 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     2521 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggatgta
     2581 gcgatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     2641 ctggacgcca acaatggcga ggtcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     2701 ggtagcgtac ccaaaatggg cgctgatgaa acccgcgctg ctatcgacgc cgccaaccgc
     2761 gccctgcccg cctggcgtgc gctcaccgcc aaagagcgcg ccaacattct gcgcaactgg
     2821 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     2881 aaaccactgg cagaagcgaa aggcgaaatc agctacgccg cctcctttat tgagtggttt
     2941 gccgaagaag gcaaacgtat ttatggcgac accattcccg gtcatcaggc cgataaacgc
     3001 ctgattgtta tcaagcagcc gattggcgtc accgcggcta tcacgccgtg gaacttcccg
     3061 gcggcgatga ttacccgtaa agccggcccg gcgctggcgg caggctgcac catggtgctg
     3121 aagcccgcca gtcaaacgcc gttctctgcg ctggcgctgg cggagctggc gagccgcgcg
     3181 ggcattccgg ctggcgtgtt taacgtggtc accggttcgg cgggcgcggt gggtaacgaa
     3241 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     3301 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     3361 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcactggcc
     3421 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     3481 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg taagcaaact gcacatcggc
     3541 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     3601 gtggaagagc atattgccga tgcgctggag aaaggcgcgc gcgtggtttg cggcggtaaa
     3661 gcacatgaac gtggcggtaa cttcttccag ccgaccattc tggtgaacgt tccggctaac
     3721 gccaaagtgt cgaaagaaga gaccttcggc cccctcgccc cactgttccg ttttaaagat
     3781 gaagccgatg tgattgcgca ggccaatgac accgagtttg gccttgccgc ctatttctac
     3841 gcccgtgatt taagtcgcgt cttccgcgtg ggcgaggcgc tggagtatgg catcgtcggc
     3901 atcaataccg ggattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     3961 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     4021 atcggtcttt aactggagaa cgcgaatgag cagcaataaa gagttaatgc agcgccgtag
     4081 tcaggcagtt cctcgtggcg ttgggcaaat tcaccccatt ttcgctgatc gcgcagaaaa
     4141 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg gcattgcggt
     4201 gctcaatacc gggcacctgc atccgaaagt ggttgcggcg gtggaagcgc agttgaaaaa
     4261 attgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     4321 tatgaatcag aaggtgccgg gcgattttgc caagaaaacg ctgctggtta cgaccggttc
     4381 cgaagcggtg gaaaacgcgg tgaaaatcgc ccgcgcagcc accaaacgta gcggcaccat
     4441 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     4501 gaatccatac tctgcgggca tgggcctgat gccagggcac gtttatcgcg cgctttatcc
     4561 ttgcccgctg cacggtatca gtgaagatga cgctatcgcc agcatccacc gaatttttaa
     4621 aaatgacgct gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     4681 cggtttctac gccgcgacgc ctgcttttat gcagcgttta cgcgcgctgt gtgacgagca
     4741 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     4801 tgcgatggag caaatgggcg tggcaccgga tctcaccacc tttgcgaaat cgatcgcagg
     4861 cggcttcccg ctggcgggcg tcaccgggcg cgccgaagtg atggatgccg tcgctccagg
     4921 cgggctgggc ggcacctatg ccggtaatcc gattgcctgc gtggctgcgc tggaagtgtt
     4981 gaaggtgttc gagcaggaaa atctgctgca aaaagccaac gttctggggc agaagttgaa
     5041 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     5101 agcgatgatc gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     5161 caccgccgag atcgtggcgc gcgcccgcga taagggcctg attcttctct cctgcggccc
     5221 gtattacaac gtgctacgca tccttgtacc actcaccatt gaagacgctc agatccgtca
     5281 gggtctggag atcatcagcc agtgttttga tgaggcgaag cagtagcgcc gctcctatgc
     5341 cggaggcgac gctgcgcgtc ttgtccggcc taggggatcc tggtcggata aggcgtttac
     5401 gccgcatccg acaatctgta cgtgaacagg aataaatcta tgttggccgg gtaaggcgga
     5461 gccgctctcc ggcaaaaaga atcaataaca attaaacgcg tgacccgacg cgggaaatgt
     5521 cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca atcatcgcaa
     5581 ccacatgagt taggcggcgg gctgaagtca cgccatgtca ccatgttgtc tattgccggt
     5641 gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc gggcccggcg
     5701 gtattactgg cctatctgtt cgccgggcta ctggtggtta tgattatgcg gatgttggcg
     5761 gaaatggcgg ttgccacgcc cgataccggt tcgttttcca cctatgccga taaagccatt
     5821 ggtcgctggg cgggctatac catcggctgg ttgtactggt ggttttgggt actggttatc
     5881 ccgctggaag ccaacatcgc cgccatgatc ctgcactcgt gggttccagg cattcccatc
     5941 tggttatttt ccctcgtcat tacccttgcc ttaactggca gtaatttatt aagcgttaaa
     6001 aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat cctggccttt
     6061 attttccttg gtgcagtcgc aattagcggt ttttacccgt atgccgacgt gagcgggatc
     6121 tcaagattgt gggatagcgg cggctttatg cccaacggct tcggtgcggt attaagcgcg
     6181 atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc cgccgcggaa
     6241 tccgacacgc cggaaaaaca tattgtccgc gctaccaact cggttatctg gcgtatttct
     6301 atcttctatt tgtgctctat ttttgtcgta gtggcgttaa taccgtggaa tatgccgggg
     6361 ctgaaagccg ttggttctta tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa
     6421 ttaatcatgg actgcgtgat attactttcc gtaaccagct gtctgaactc ggcgctgtat
     6481 accgcgtcaa ggatgctcta ctccttaagc cgtcgcggtg atgcgcccgc ggtaatgggc
     6541 aaaatcaacc gcagtaaaac cccgtatgtg gcggtgttac tctccaccgg agcggcattt
     6601 ttaacggtgg tggtgaacta ttacgcacct gcgaaagtgt ttaaattcct gatagacagc
     6661 tccggtgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgt
     6721 aaaattctgc gagcagaagg aagcgaaatt cgcttgcgca tgtggcttta cccgtggctc
     6781 acctggctgg taataggctt tattaccttt gtgttggtgg tgatgttatt ccgcccggcg
     6841 caacagttag aagtgatctc cactggctta ttagcgatag ggattatctg taccgtgccg
     6901 attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc
     6961 tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga
     7021 agaacgatat tattcgcggt aattttcaac cggatgaaaa attacgaatg aatttgctga
     7081 catcgcgtta tgcacttggc gttgggccgt tacgggaagc tctttcgcaa ctggtggcgg
     7141 aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg
     7201 agctgctcga tattttcgac gcccgcgcca atatggaagc gatgttagtg agtctggcga
     7261 ttgcccgcgg tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctgagta
     7321 agcttgaggc ctgtgacgct agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg
     7381 cgtttcatac tgcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac
     7441 ggttgtttga tctggcggcg cgttatcgat ttatctggct gcggcgaacg gtgctttcgg
     7501 tggaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc
     7561 gagataccgc gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta
     7621 tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga
     7681 taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga
     7741 ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta
     7801 taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaatg
     7861 ccggaaatat tccccaccgc aacaaggatt ttctctttcg cctcctgact aagaccgtca
     7921 ccagtgaccg tcgctttgcc gtcggcaatt tgaatattca ctttatcggc atcaggtata
     7981 ccggttttgt tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct
     8041 gtaaccgcgt cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct
     8101 acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc
     8161 cgcggggatg agtacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg
     8221 ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac
     8281 cgagcagcac gccgagcggc ggcagaatga tggtgatgac gattctccag aaacccatat
     8341 gtactcccta taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt
     8401 aattttagcg gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg
     8461 ctggtatatg ttgcattttt agtttagagt ttacttaatt tagcaaatac ttaaataact
     8521 atgactgaac tcgcgcaatt acaggctagt gccgaacagg cagcggcctt attgaaagca
     8581 atgagccacc ctaaacggtt gctgattctg tgcatgctta gcggttcccc cggcaccagc
     8641 gcgggagagc tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgcc
     8701 cgtatgcggg acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc
     8761 attaaaaatg aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa
     8821 ggagtcacta tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacac
     8881 ggcgcaaagt taatcgatat tcgtgatgct gatgaatatc ttcgtgaaca tattcctgaa
     8941 gcagatctgg ctccattatc tgtgctggaa cagtcaggtc tcccggctaa attacgtcgc
     9001 gagcaaatta tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta
     9061 gcagcgattg ccgcccccgc agaaatcttt ttactcgaag atgggattga tggctggaaa
     9121 aaagcaggat taccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg
     9181 cagatcgctg cgggtggttt aatattaatc ggcgttatac tgggttatac cgtaactagc
     9241 ggtttcttct tattaagcgg ctttgttggt gccgggttac tgtttgcagg aattagcggt
     9301 ttttgtggga tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg
     9361 acgccggacg cgccatagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga
     9421 gagatttacc ttctgccagc gcctgagcga ttggcttcgg tgtacggccc tgaccggtcc
     9481 aggttttagt ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt
     9541 ttttaccagc gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc
     9601 cgtcagcttt catcagctcc agccaggtgc taattttttc ctggcgctct gccagttcac
     9661 gctgctgctg ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca
     9721 tttcttcaag aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat
     9781 ttaaactttg taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag
     9841 ta
//
LOCUS       DAZUGK010000167         9804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZUGK010000167 DAZUGK010000000
VERSION     DAZUGK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.168"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..2684
                     /locus_tag="ACHY5W_003629"
                     /pseudo
     CDS             134..2684
                     /locus_tag="ACHY5W_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
     gene            complement(2740..3075)
                     /gene="ymgD"
                     /locus_tag="ACHY5W_003630"
     CDS             complement(2740..3075)
                     /gene="ymgD"
                     /locus_tag="ACHY5W_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFS4448454.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3079..3423)
                     /gene="ymgG"
                     /locus_tag="ACHY5W_003631"
     CDS             complement(3079..3423)
                     /gene="ymgG"
                     /locus_tag="ACHY5W_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFS4448455.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3425..3598)
                     /gene="ymgI"
                     /locus_tag="ACHY5W_003632"
     CDS             complement(3425..3598)
                     /gene="ymgI"
                     /locus_tag="ACHY5W_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFS4448456.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3699..3884
                     /locus_tag="ACHY5W_003633"
     CDS             3699..3884
                     /locus_tag="ACHY5W_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448457.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYSTTFRR
                     TAFKKCLFLMRDIIISS"
     gene            3845..3964
                     /locus_tag="ACHY5W_003634"
     CDS             3845..3964
                     /locus_tag="ACHY5W_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4448458.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            3999..4343
                     /locus_tag="ACHY5W_003635"
                     /pseudo
     CDS             3999..4343
                     /locus_tag="ACHY5W_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4715..4981)
                     /gene="minE"
                     /locus_tag="ACHY5W_003636"
     CDS             complement(4715..4981)
                     /gene="minE"
                     /locus_tag="ACHY5W_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFS4448459.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(4985..5797)
                     /gene="minD"
                     /locus_tag="ACHY5W_003637"
     CDS             complement(4985..5797)
                     /gene="minD"
                     /locus_tag="ACHY5W_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFS4448460.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5821..6516)
                     /gene="minC"
                     /locus_tag="ACHY5W_003638"
     CDS             complement(5821..6516)
                     /gene="minC"
                     /locus_tag="ACHY5W_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFS4448461.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7036..7404
                     /gene="ycgJ"
                     /locus_tag="ACHY5W_003639"
     CDS             7036..7404
                     /gene="ycgJ"
                     /locus_tag="ACHY5W_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFS4448462.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7507..7908)
                     /gene="pliG"
                     /locus_tag="ACHY5W_003640"
     CDS             complement(7507..7908)
                     /gene="pliG"
                     /locus_tag="ACHY5W_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFS4448463.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8150..8443
                     /locus_tag="ACHY5W_003641"
     CDS             8150..8443
                     /locus_tag="ACHY5W_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFS4448464.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8515..9174
                     /gene="ycgM"
                     /locus_tag="ACHY5W_003642"
     CDS             8515..9174
                     /gene="ycgM"
                     /locus_tag="ACHY5W_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS4448465.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9266..9712
                     /gene="ycgN"
                     /locus_tag="ACHY5W_003643"
     CDS             9266..9712
                     /gene="ycgN"
                     /locus_tag="ACHY5W_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFS4448466.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         2590 a         2146 c         2383 g         2685 t
ORIGIN      
        1 atatattgtc gctgtttatc tatttaaatt ttttgtgctt ttgtttttgt ctttaccctc
       61 cgttttggta atctgaactc ataggttatt tgaccaggaa tgtttgttaa tccaatgaat
      121 ggatggttaa tctatgaaac tgaaaaaact ccccgggttt agtttaggac ttattgctct
      181 ggcggtgggt aatgcatatg ctgattagtt tattcatcac gatgagtata aacatgctgt
      241 ggaaataaaa gatggagaag taaaagatat tgataatgcg ctagttataa caacgggtaa
      301 tggaagttac ggtgtatccg ttgcaggaga gggtagcaat ttagccatta acaatggcgt
      361 cattgtaaca acgggcggtt tatatccttc tgacaatagt cctctaagca aagcatatac
      421 agctagcgca gtcgtaagtg aatatggcgg tgctgtcact ttgcggggta ataatacgat
      481 tgtcacaacg ggggattact caattggttt gctaagtcaa acgagtggaa acctgaatac
      541 tgataccata ataagagtca actctgacgg ttcggttacc ccctcttttt ctgacggaga
      601 taatactttt attgttactg cgggtaatca cgcagtcggc gttcttgcgt gtgcatctcc
      661 cggaagcgct cgtgcgtgtg tatcttctct tgatgaagaa agcactgccg atacaggaaa
      721 taatgaaaat agcgccaaag caatactgga tatggcaaaa ggggagataa caacccacgg
      781 aacagaaagt tggggacaat gctcatgcca tcgctgctcg ccaggggaca gtttcattta
      841 accagggaga aatttataca acgggtcctg acgccgcgat tgctaaaatt tataatggcg
      901 gcacggtgac gctgaaaaat acatttgcag tcgcgcatca gggggctgga gttgtgctgg
      961 agtcttccat aaatggtcag gaagcaacgg tagatatttt atctggtagc tcactacggt
     1021 cagcaaatga aatcctctac aataaaaatg agacgagtaa cgtgaccatt actgatagtg
     1081 aggtatcatc ggctgcagat gtttttatca ataatattaa aggtcatttg accgtctatg
     1141 caactgattc aaaaataacg ggttcagcca atctttcaac agatgataac actcatacct
     1201 atctgtcgct ttcagataat agtacctggg atattaaagc tgactcaacg gtgagcaagc
     1261 tcacggttga caatagtacg gtctatattt cccgggcaga tggaaaggat ttcgaaccaa
     1321 caagattaac aataactgaa aattacgttg gtaataatgg cgtattgcat ctcagaactg
     1381 aattgggtga tgataattca gctacggata aagtcgttat taatggaaat acctctggaa
     1441 caacccgagt taaagttacc aatgctggcg gcagcggggc ttatacgtta aatgggatag
     1501 agattatcag cgttgagggg gaatcaaatg gagaatttat taaggattcg aggattttcg
     1561 ccggtgccta cgaatattca ttaacccgag gtaataccga agcgaccaat aaaaactggt
     1621 atctgactaa cttccaggca acgagcggcg gtgaaacaaa ctccggagga agttcagcgc
     1681 ctactgttgc gcctaccccc gtcctgcgcc ccgaagctgg aagttacgtc gccaacctgg
     1741 cagccgctaa cactcttttt gttatgcgtc tgaacgatcg tgcaggtgaa acgcgttaca
     1801 tcgatcctgt aactgaacag gagcgttcaa gccgactttg gctacgtcaa attggcgggc
     1861 ataatgcctg gcgtgacagc aacggacagt tgagaacgac ctcgcatcgc tacgtctcgc
     1921 agttaggggc cgatctgtta accggtggtt ttaccgacag tgacagttgg cgtttgggag
     1981 tgatggctgg ttatgcccgc gactacaact caactcattc cagcgtgtcg gattatcgtt
     2041 cgaaagggag tgtcagaggc tatagcgcag ggctgtatgc cacctggttt gccgatgaca
     2101 tcagtaaaaa aggcgcatac attgacgcct gggcgcaata tagctggttt aaaaactcgg
     2161 tgaaagggga tgaattagcc tatgaatcct atagcgcgaa aggcgcaacc gtctcgctgg
     2221 aagcgggtta cggctttgcc ctgaataaat cctttggtct ggaagcggcg aaatatacgt
     2281 ggatcttcca gccacaggca caggctatct ggatgggcgt cgatcataat gcgcacaccg
     2341 aagccaatgg ctcacgtatt gagaatgacg caaataacaa catccagacc cgacttggtt
     2401 tccgcacttt tattcgtact caggagaaaa acagcggtcc gcacggtgac gactttgaac
     2461 cttttgttga aatgaactgg atccataaca gtaaagattt tgctgtctca atgaacggtg
     2521 tgaaagtcga acaagagggg gcgcgtaatc tgggggaaat taaacttggc gtaaatggca
     2581 atctgaatcc ggcggccagc gtctggggta atgtgggcgt gcagctgggt gataatggct
     2641 acaatgacac cgcagtgatg gtgggcctga aatataagtt ctgatccccg ttagctaaaa
     2701 taccgcgtcg aattcatcga cgcggtaaca tatgaaatat tattttttgc cgatagcacg
     2761 catggtgtca tcaattgccg tgatcaacag catttgcggg tctttagcgc aaacctgatt
     2821 cagtttttct accactttgg cgctcagttc cggagattgc tcaattttta aatcacggct
     2881 ggcaacgctg gcattaccca ttaccgcaac aatttctgca acctgtgcgc tgtcagtttt
     2941 tgccatttcg ttggcttctg cgcaagtaat ataggtttct gacggcaaac cgtttttaat
     3001 attgtagtcc tgcgcccagg tcattggtgc gaaaacaaac aggcccgcca gtaaagcaaa
     3061 ttttttcatc atcattcctt atttcatttt acccagaatt gcaccacccg taccgccaat
     3121 cacggcacct ttaatcgccc cttcgaggcc attgccggtc agaacgccag tgacagcacc
     3181 aacggcggca cccacttttg cacctttacg cgcattttta ccgtcgcggc ctttttctgt
     3241 tactgcacca acaccagcgc caacagctgc acctttcagt acgccattaa caccattgcc
     3301 agtaagtaaa ccaacgcctg cgcctagcaa tgcacctttc gtggtgcggt tcatatccgc
     3361 catcgctggc gtggagcaga acaatgctga gataagcccg aaggcaagta tttttttctt
     3421 caacttagat gtccggtatt aagtaagttg cacacacaat aatttcgtct tcaattaaga
     3481 tctgcttaac taaagaacgc tcgctattat tcagataatt caaaatgagc gtggctgtga
     3541 tgataggaat tatgtttttt acgtgaatga gaataatctt aaatgaggaa taactcattg
     3601 attgacaata tttttattca agaagcgtca ttgactgtta acgcaatgtt gtaaaggtaa
     3661 gataatctga tttatcaata ttattgtgtg atttttatgt gagcagaaga tattcatcag
     3721 caacgattac attagtcatt ttattttgcc gacggcctca ttgtcgaaag ataagcgtac
     3781 gacagtatta tctgaaaaga gtgatttttt attcaactac atttcggcgc actgcgttta
     3841 aaaaatgcct ctttcttatg cgggatatca tcatttcatc atgatgtctt tggtgagcgg
     3901 tgaacacaat acacctgcgc tgtctcttca ggatgaatcc cctggtctgg tgcctgcggg
     3961 ctgatgttgc agccgagcta aggtcactta gacgctacta tcatttatcc aatggcatgg
     4021 aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggtgcaacg ctgagttaca
     4081 acatgcgtct ggggaacggt atggaagttg agccgtggct gaaggcggct gtgcgcaaag
     4141 aatttgtcga tgataaccgg gtgaaagtga ataatgatgg taatttcgtc aatgatttgt
     4201 cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt acgttaagcg
     4261 ggcatctcgg ggtggggtat agccgtggtg ctggtgtgga atccccgtgg aatgcggtgg
     4321 ctggtgtgaa ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc gcagttttcg
     4381 tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa gcccgccagt tacggcggga
     4441 aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat ctcccagtat
     4501 atccatgcta acaataaggt tatttactca accaggcata aacattttgt tttgtgcgtg
     4561 ggaacagcct taaggtgtaa agggggaggt ggaaatagca atgaggagta tcagcaagaa
     4621 tactcgccgc tttaccacaa cgtggatgag agggatgaaa aactcaaggc agagataact
     4681 ctgccttgaa gataaatgcg cttttacagc gggcttattt cagctcttct gcttccggta
     4741 aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc tgtacggtta
     4801 ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct ttacgcaact
     4861 gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata atctgcagcc
     4921 gttcttttgc aatgttggct gtgtttttct tccgcgagag aaagaaatcg agtaatgcca
     4981 taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca atgaagcgga
     5041 aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta cccgcatcgg
     5101 cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact gattgatcct
     5161 ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc atgctcagca
     5221 tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc tctttaatag
     5281 gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa atacggtcag
     5341 agtcgcgtac tgaagagact tccgggttgg tggtaataat ggcttcgtct gcaaaataga
     5401 gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata aattcaaaat
     5461 ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg gcatctttat
     5521 cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct ttaattaacg
     5581 cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg acccggcgtt
     5641 cgcaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc acgacagttt
     5701 tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc ttaccaacac
     5761 cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt taaaaaggga
     5821 tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg ccgctttgcc
     5881 ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg acaccagttc
     5941 cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc ctgccagcgc
     6001 acgaccgcgc atcatgccat agacatgaat gttaccatcg gcaatcaatt cggccccagc
     6061 gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct gaccggaacg
     6121 caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg ctggagcctg
     6181 cggtgcggga gctggacgcg gcgctttttc ctttccttcc gtcaggatag gcagccccat
     6241 cttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa taacccgcaa
     6301 accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt ccagtgcact
     6361 gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga ttttgtcttc
     6421 cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag ataaagtgaa
     6481 gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca attagctatt
     6541 aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata gtctggatta
     6601 tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt gataaatggc
     6661 gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg tacttttgat
     6721 tgttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctt cagacctgtt
     6781 taaatatcca gcattgaggc aagtgtattt tttcaccacg ccaggctttc ttaactttcc
     6841 attttttgca gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg
     6901 ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc
     6961 aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg tacggttgga
     7021 ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc aggtgtggga
     7081 atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc
     7141 ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc agagttaaca
     7201 aggaaatatc tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc
     7261 agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgccgtgat
     7321 gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag
     7381 atgttattta tgtgtcgtga gtgatgtctt gtttacatta tgttctattc gtcattaacc
     7441 tcttgagcga cagagaggag tggggcgtca acgtttatga cggggaggct ccccgtcggt
     7501 tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt tacgggcgtc
     7561 gtttcgggtt tgcagcactc gcagctcata tttgccagaa gcagggagtg agtattgtcc
     7621 gtgactatcg agttcagggg aatatctgga taaatcaaca gaatcatcga ttcctggccc
     7681 gaagaggtaa gtatcagcgc cttcattaga gatactcaca tgcactttct gacctttttt
     7741 ggcatagaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt actgagcgct
     7801 gctatggcct tttctgaact caacattgac attcttaccc gcagcaaagc tcgtggaggt
     7861 tagcagtgca agcagcaata cagccttact gatgctcttg attttcattg gtagtctcct
     7921 gttcagaatg agtacgatga actgttataa tataacaatc ccttacggga gaattccagt
     7981 ggctttcctg cgttcggtaa aatcccgtcg cggcattttt gccgggggga ttacttataa
     8041 agggggcatg gactcaacaa aacgtagaaa tctacagctg gctggcttgt ggcgcgggtt
     8101 tcattgatgt tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt
     8161 gatttatcga agcagcaagc gtgaccagac ctatttatat gtcgaaaaaa aagacgattt
     8221 ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct
     8281 gccgctggat gggcggaaga aactggtcaa tgccgatatt gaaaaggtta aacaggcatt
     8341 aaccgagcaa ggttactatt tgcagttacc gccaccaccc gaagatttgc tgaagcaaca
     8401 tctttccgtc atggggcaga aaacagacga cactaacaaa taaccgatat ccggcggtgg
     8461 cattatcttt gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat
     8521 caacatcaca actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtctgtgtt
     8581 ggcagtaact atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg
     8641 ctgtttatta aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc
     8701 gatttcggtt cagttcatca tgaagtcgaa ctggcggtgt tgattggcgc gacgctgcgt
     8761 caggctacgg aagagcatgt ccgcaaagcc attgccggtt acggcgtggc gctcgatctg
     8821 acgttgcgtg atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggctaaa
     8881 gcgtttgata actcttgtcc gctttccggg tttattcccg cggcggaatt caccggcgat
     8941 ccgcaaaata caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact
     9001 gcggacatga tccataaaat cgttccgctg atcgcttata tgagcaagtt ttttaccctc
     9061 aaggccggtg acgttgtgct gacaggcacg cctgatggcg tcggcccgtt gcaaagcggt
     9121 gatgagctga cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt
     9181 ttgccgcctg aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg
     9241 ttttaacgta atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct
     9301 ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct
     9361 gcataaactg atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca
     9421 gctcaatatt aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacccga
     9481 ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa tgacctgcgc
     9541 ttatcgtttg ctggcggaag gtaaagattt acctgcgtgg catccgctgc ttactggttc
     9601 gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga
     9661 agtgattgac tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta
     9721 atagatgttg atgggggctt tcttaatata agtctggatg ctcgccattt cgtagccctg
     9781 tttttgaaag cgctctgtca taat
//
LOCUS       DAZUGK010000168         9801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZUGK010000168 DAZUGK010000000
VERSION     DAZUGK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.169"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1017
                     /gene="tsuA"
                     /locus_tag="ACHY5W_003644"
     CDS             <1..1017
                     /gene="tsuA"
                     /locus_tag="ACHY5W_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFS4448467.1"
                     /translation="LGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFA
                     LIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTY
                     MVMSAVMRSPHVSGLNQTLQHYTTEHNSIADTFNLSVWPLVAVLLVITLWVVMKELKK
                     PKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSP
                     TANILQFLVAGDVKYINWGVFLVIGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGV
                     LMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLAT
                     AAAN"
     gene            1031..1258
                     /gene="tsuB"
                     /locus_tag="ACHY5W_003645"
     CDS             1031..1258
                     /gene="tsuB"
                     /locus_tag="ACHY5W_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFS4448468.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1301..2728)
                     /gene="sbcB"
                     /locus_tag="ACHY5W_003646"
     CDS             complement(1301..2728)
                     /gene="sbcB"
                     /locus_tag="ACHY5W_003646"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFS4448469.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2937..4103
                     /gene="dacD"
                     /locus_tag="ACHY5W_003647"
     CDS             2937..4103
                     /gene="dacD"
                     /locus_tag="ACHY5W_003647"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFS4448470.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEAKKLLR
                     WGQQNFTTVQILHRRKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLN
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4222..4695
                     /gene="sbmC"
                     /locus_tag="ACHY5W_003648"
     CDS             4222..4695
                     /gene="sbmC"
                     /locus_tag="ACHY5W_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFS4448471.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            4893..5951
                     /gene="yeeA"
                     /locus_tag="ACHY5W_003649"
     CDS             4893..5951
                     /gene="yeeA"
                     /locus_tag="ACHY5W_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4448472.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEINTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            6123..6452
                     /gene="tmaR"
                     /locus_tag="ACHY5W_003650"
     CDS             6123..6452
                     /gene="tmaR"
                     /locus_tag="ACHY5W_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFS4448473.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6553..6735)
                     /locus_tag="ACHY5W_003651"
     CDS             complement(6553..6735)
                     /locus_tag="ACHY5W_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFS4448474.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            6789..6935
                     /locus_tag="ACHY5W_003652"
                     /pseudo
     CDS             6789..6935
                     /locus_tag="ACHY5W_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7224..7304)
                     /locus_tag="ACHY5W_003653"
                     /pseudo
     CDS             complement(7224..7304)
                     /locus_tag="ACHY5W_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7350..7544)
                     /gene="yeeW"
                     /locus_tag="ACHY5W_003654"
     CDS             complement(7350..7544)
                     /gene="yeeW"
                     /locus_tag="ACHY5W_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS4448475.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(7541..7914)
                     /gene="cbtA"
                     /locus_tag="ACHY5W_003655"
                     /pseudo
     CDS             complement(7541..7914)
                     /gene="cbtA"
                     /locus_tag="ACHY5W_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(8003..8371)
                     /gene="cbeA"
                     /locus_tag="ACHY5W_003656"
     CDS             complement(8003..8371)
                     /gene="cbeA"
                     /locus_tag="ACHY5W_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFS4448476.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(8445..8666)
                     /gene="yeeT"
                     /locus_tag="ACHY5W_003657"
     CDS             complement(8445..8666)
                     /gene="yeeT"
                     /locus_tag="ACHY5W_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS4448477.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8729..9205)
                     /gene="yeeS"
                     /locus_tag="ACHY5W_003658"
     CDS             complement(8729..9205)
                     /gene="yeeS"
                     /locus_tag="ACHY5W_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS4448478.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9221..9700)
                     /locus_tag="ACHY5W_003659"
     CDS             complement(9221..9700)
                     /locus_tag="ACHY5W_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS4448479.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         2369 a         2315 c         2567 g         2550 t
ORIGIN      
        1 ctgggatttg tcatgcagcg tgggcgtttt tgcctgacag gtggttttcg cgatatgtat
       61 atcgcgaaaa ataatcgaat gttttacgcc ttgctgattg caatttcggt acaaagcgtg
      121 ggggtttttg cgttaattca ggcgggccta ctgacttacg aagccggggc gttcccgtgg
      181 cttggtactg ttataggtgg gtatcttttc gggctgggaa ttgttctggc gggtggatgt
      241 gccaccggga cctggtatcg cgcgggtgag ggattgatcg gcagttggat cgcgcttttc
      301 acttatatgg tgatgagtgc ggtgatgcgt tctccacacg tcagtggttt aaatcaaacc
      361 ttgcagcact acactactga acataattct attgctgata cttttaattt gtctgtgtgg
      421 ccgttggttg ccgttttgct ggtgataacg ctctgggtgg tgatgaaaga gttgaagaag
      481 ccaaaactca aagtcgcgac cttaccgccg cgccgaaccg ggatcgctca tattctgttt
      541 gaaaaacgct ggcatccctt tgtcactgct gtactcatcg gtttgattgc gcttttagcc
      601 tggcccctga gtgaagcaac cggacgcatg tttgggttag gaatcacttc cccaacggcc
      661 aatattctgc aatttctggt cgcgggtgac gtgaaataca ttaactgggg cgttttcctg
      721 gtgataggga tcttcgtggg gtcatttatt gcagccaaag cgagccgtga gttccgcgtt
      781 cgcgctgctg atgcacaaac aacattacgt agcgggttag gtggtgtact gatgggcttc
      841 ggtgccagca ttgcaggtgg ttgctctatc ggtaatggac tggtcatgac tgcaatgatg
      901 acctggcagg gctggattgg ccttgtattt atgattctcg gagtctggac tgcgtcctgg
      961 cttgtgtatg ttcgaccgca gcgtaaggca cgactggcaa ctgctgcggc aaattaagta
     1021 aggtgtaaag atggcgatta aaaagctgga tgtggtgacg caggtctgtc cgtttccact
     1081 catagaagca aaagcagcac tggcagaaat ggccagcggc gatgagttgg taattgagtt
     1141 tgattgcacc caggcaactg aagcaatccc acagtgggcg gcagaagaag gacatgccat
     1201 caccgattat cagcagattg gcgatgcagc gtggagcatc accgttcaaa aagcctgact
     1261 caaaattgtc ctccgccgta ccagcggcgg aggcttcaca ttagacaatc tcttccgcgt
     1321 actgccaaag cgcttttaac agcgccactt tttctttatc gtcggcatac tgctgcgcca
     1381 gcatctgaat ctcttctgca tagccttgta aaaactctgg cgtgaagacc tggcggcggt
     1441 gctccagcca gcgttgctgc tcggcataat ccagcgtccc cgggaagttg cgcgcccgat
     1501 aattgaacaa cagcttttca attcgtttat caacaaaagt gatatccagt gccggtaaat
     1561 tacgcggctc ggtttccaac acaattttca ttgctgcacg atcggcgtca ctgaaaaagc
     1621 cgttataaag ctgtgcatcc acattatctg aaggtgtaaa cggttcggct tccacgaata
     1681 tcgctaccac tttttcacgc acttgcggat tttcacgcag aatttttagg ttatcgaggc
     1741 aatgctgacg attaattccc agtcggtcgg catcttccgg gcgtagcgta ttcgcctggg
     1801 ccagtaccgg acatttattg atatgcacca gcttaaccgg aacggcggcg ttatcgccaa
     1861 gatcggcttt tgcggtatat aaacgctcgc gcaatgtgtc gctatccagc tccagtaatg
     1921 gcgaaatgtc tcctgccaaa tccaccataa ttacggcatt gcgattttca ggatgccacg
     1981 ccagcggtgc cacccagctt gtattgccgc gccatgcccc aaacattccg gaaacgtgaa
     2041 ccaggggttt catctgcgga acatcaatca acgccattag tttgtgttta ttacgatggg
     2101 taaagagata atcaaacagg cgtggctgac gcgtttttac cagttttgcc atcgcaatag
     2161 tggcgtacac atcagccatc gcatcgtggg cgttgctatg ttcaatacca ttcgctttgg
     2221 ttaaatgctc aaggcgaaag ctcggtagac cgtcatcatt ttcaggccaa tttattcctt
     2281 ccgggcgcag ggcataacag gcacgcataa catccagtaa atcccagcgc gagttatcat
     2341 gctgccagct ccaggcgtaa ggatcgtaga aattacgata aaaaacgttg cgtgtgactt
     2401 cgtcgtcgaa acgcacattg ttgtagccca gaatacaggt cttcggtacg gtaaaaagcg
     2461 agtgaatacg ggcggcaaac gcggcttcgt tttctccttt cgctcgtgcc tcctgcgggg
     2521 taataccggt aattaatact gctccaggct ggggtaaata gtcatccgcg ggcttgcagt
     2581 aaaagacttc gggttcgccg atgacattga attcgtcatc ggtgcgaatg gctgcgaact
     2641 gtgcagggcg atctaacgcg gggtgcgtgc caaaggtttc gtaatcgtga aacaaaaagg
     2701 tagattgttg cttaccgtca ttcatcatta ggttaaatcc gttatttctg ctgtttgcca
     2761 gagtatcaaa tatcaccgtg ctaatcagct ttagcgcaac catttgacag cgagtggcaa
     2821 cagatcatgt cagataaaaa tgagagggta gtcacatttt cttgcacttt attccagcca
     2881 gttcataagt atttccgtaa aaagaacagc tatttgaaac tcctgagggt ttgctgttga
     2941 aacgccgtct tattattgct gcttctttgt tcgtttttaa cttatcgtct ggttttgcgg
     3001 cggaaaacat tcctttttca cctcagcctc cagagattca tgccgggtcc tgggtactga
     3061 tggattacac caccggacag atcctcaccg cgggtaatga gcatcaacag cgcaatcccg
     3121 ccagcctgac aaagctgatg acgggttatg tcgtggatcg cgctatcgat agtcatcgca
     3181 ttacgccaga cgatattgtc accgtggggc gcgatgcgtg ggcgaaagat aatccggtgt
     3241 ttgtcggttc ttcactgatg tttttgaaag agggcgatcg cgtatcggta cgtgatttaa
     3301 gccgtggttt aattgtggat tccggaaatg acgcttgtgt tgctctggct gactatattg
     3361 ccggtgggca acggcagttt gttgaaatga tgaacaacta tgccgagaag ctgcatctca
     3421 aggatacgca ttttgaaaca gtgcatggtc tggatgcacc tggccagcat agctcggctt
     3481 atgatttagc cgtgctttct cgcgctatca tccacggcga gcccgagttt tatcatatgt
     3541 acagtgagaa aagtctcacc tggaacggta tcacccagca aaaccgtaac gggttattgt
     3601 gggataagac catgaatgtt gacggcctga aaacgggcca tacttctggt gccgggttta
     3661 atcttattgc ttcggctgta gacgggcagc gtcgcctcat tgcagtggta atgggtgctg
     3721 acagcgcaaa aggtcgtgag gaagaggcaa aaaaattact gcgttggggg caacaaaact
     3781 ttactacggt gcaaattttg caccgtagga aaaaggtcgg tacggaacgc atctggtatg
     3841 gtgataaaga aaatatcgcc ctgggaacgg aacaagagtt ctggatggtg ctaccgaaag
     3901 ccgaaattcc acatatcaaa gccaaatata cccttaatgg taaagagctc accgcgccaa
     3961 ttagcgccca tcagcgggta ggggaaattg aactttacga ccgtgataaa caggtggcgc
     4021 actggccgct ggttaccctg gaatctgtcg gggaaggcag catgttttct cgcctgagtg
     4081 attatttcca ccataaggcc tgacctttct tttgcagcag actggcagga gtgcgagtct
     4141 gctcgcataa tcaacactca ttccttgtgg ttttaatatt gcaactatac tgtatataaa
     4201 aacagtatta atggaggcgt catgaactac gagattaagc aggaagataa acgtaccgtt
     4261 gcaggtttcc atctcgttgg cccgtgggaa cagacggtaa agaaaggttt tgagcagttg
     4321 atgatgtggg tagatagcaa aaatattgtg ccgaaggagt gggttgctgt ctattacgac
     4381 aatccggatg aaacacccgc cgaaaaatta cgctgcgaca ccgtcgtgac ggtaccgaat
     4441 aactttacgc tccccgaaaa cagtgagggc gtcattctga cagaaatttc aggtggtcaa
     4501 tatgccgtgg cggtggctcg tgtagtcggt gatgattttg ctaaaccctg gtatcagttc
     4561 tttaatagcc tcttgcagga cagtgcttat gaaatgttac caaagccctg cttcgaggtt
     4621 tatttgaaca atggcgcgga agatgggtac tgggatatcg aaatgtatgt tgcggtgcag
     4681 ccaaaacatc actaattcat ctcagggcgg tgtgttaacg ctatgaccac tctttttttg
     4741 aaagcgaaaa gagtaagatg cgcctttcaa ttttttcgct cctgccggga aattacactg
     4801 ttcccggttt gtccgtcgga taattcagag gcgcgccttc tggccgacag atgagttatg
     4861 agcgctttta atcttcatta cggagtttct gcgtgcgtgc cgataagtca ttaagcccgt
     4921 ttgaaatccg ggtataccgc cattaccgca ttgtgcatgg tactcgggtc gcgctggcat
     4981 tcctgctcac ttttctcatt atccgcctgt ttactatccc ggaaagcacc tggccgctgg
     5041 tcaccatggt ggtgattatg gggccaatct cgttctgggg gaacgttgtc cctcgcgcat
     5101 tcgaacgtat tggcggtacg gtgttggggt cgattttagg tcttatcgct ctgcaactgg
     5161 agttaatctc gttaccgctg atgttagtct ggtgcgcggc ggctatgttt ctttgcggtt
     5221 ggctggcgct gggcaagaaa ccgtatcaag gtttattgat tggggtgacg ctggcaattg
     5281 ttgttggttc cccgacaggt gaaattaata cggcgttatg gcgaagcggc gatgtgatcc
     5341 tcggctcttt actggcaatg ttgtttaccg gtatctggcc acaacgggcg ttcatccact
     5401 ggcgcattca actggcgaaa agtctgaccg agtataatcg ggtctatcaa tctgcattct
     5461 caccgaactt actcgaacgc ccacgtctgg aaagccatct acaaaaactc ctgaccgatg
     5521 ccgtgaaaat gcgtgggctg attgcgcccg ccagcaaaga aacccgtatt ccaaaatcga
     5581 tatatgaagg tatccagacc attaaccgca atctggtttg tatgctggag ttgcaaatca
     5641 atgcgtactg ggccacgcgc cccagccatt tcgtgttatt gaacgcgcaa aaacttcgtg
     5701 atacccagca catgatgcag caaatactgc tgagccttgt tcatgcgctg tacgaaggta
     5761 atccgcagcc ggtttttgcc aatacggaaa aattgaacga tgctgtggaa gagctgcgtc
     5821 agttgctcaa taaccaccat gacctgaagg tagtggaaac accaatctat ggttatgtgt
     5881 ggctgaacat ggaaacggcg catcagcttg agttgctatc gaatctgatt tgccgggcct
     5941 tgcgcaaata attcctgaac ttcagaatca tcttgctgct gcttcgattc agcaaggata
     6001 aagggtatga tagtgaaaag ggataaaagc attgtcatct gcggcagcta tgagtaatgt
     6061 tggccctaac gaatagcggt tgcttaaacg aatccgactc tcacattatc aggggtataa
     6121 aaatggaaac taccaagcct tcattccagg acgtactgga atttgttcgt ctgttccgtc
     6181 gtaagaacaa actgcaacgt gaaattcagg acgttgagaa aaagatccgt gacaaccaga
     6241 agcgcgtcct gctgctggac aacctgagcg attacatcaa gcctgggatg agcgttgaag
     6301 caatccaggg catcatcgcc agcatgaaag gtgactatga agatcgcgtt gacgattaca
     6361 tcatcaaaaa tgccgagctc tccaaagaac gccgcgatat ctccaaaaag ctgaaagcta
     6421 tgggcgaaat gaaaaacggc gaagcgaagt aattcccgtt ttattcaatg agggttgccc
     6481 ggcaaccctc attgctcatt gattcttatc tgtgtatcac cgtcatcatt ctcatctgag
     6541 aaccaatcga aattaacaac agccttcttc tgtatgcagc aaggcaaaaa gttctgtaac
     6601 tccattgtta ttaactgcac tggttactaa cacgttgtgc gctccagctt cccgtaacca
     6661 acttttcacc aaagatattt gttccatgct ggctaaatct gctttggtta ctactccaat
     6721 gaccgggtga ttcatggccc ggtagggcgt ttttacctct aattcctgct tcagcactga
     6781 gattcctgct tgttgccgga aaagcaggca cctgccgtct gaacggtatc gatccggaag
     6841 cgtatctgcg ccatattctg agcatactgc cggaatggcc ctccaaccgt gttgacgaac
     6901 tcctgccatg gaacgtagtt ctcaccaata aataagcgtc aatacggtgc tccgttgacg
     6961 cttactttaa tccgttttct gagcttagat ttttctgttc ctacccagac cgtggatttg
     7021 gaattcattt tttgtcaggt caatgctaat cgttaaaacg gccataacgc acctccactg
     7081 gaaggtttat cagtaatatc ctaccgttgc ttcatggtcg gggcgtccat tccattatta
     7141 tcggttttcc tttctgttta taaatgaaaa cgccagctgt attcaggctg gcgtcagggg
     7201 aaatgaagcc tgttgagtga gattcaccgg ttctggtgca gaagctgcag atggcgcagg
     7261 gtaaggacat cttctcttgc ctgcttaata caggacaaca gtgctgtttc tgtggttaat
     7321 ggcactatac cctgcatcac tgcattgtct tacagcaact ggtgagggct ggcataagtt
     7381 gccggtgttt cggtagagtc acggtcatcc accacgcgga gtgaataacc gttttcagcg
     7441 gccttattaa tcagttcagt gatattaaca ccatcaatgt caacgacaat gtgccccata
     7501 tccagcgcct gtacgttaac gctgtcggct tccagtgtca tcatttcgcc tccggatact
     7561 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
     7621 tgcgagcccg gaggatatcg atgctgttta ttaactgaga gcgggtacag gcgctgaatc
     7681 ccggctggtc ggtagcacca gcgcgtattt ttccacgaga aagttcaccg catcacacag
     7741 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcggcaaaag gtgtgtcatt
     7801 cagtgtgagg ccatagtgct ggtccagcag tcgggacagc agtatctgcc agatttcaac
     7861 aggagacggg cgagaactgg ccgcctgccc gggtaataca ggtaatgttt tcatactgaa
     7921 gattttcctg atatgcagat ataaaaatgg aaaagtggcg tggtgaaaac accaggccgt
     7981 agcagaaggc tattctggag agttaatttt tcatttcggg cgtcggataa acagccagat
     8041 aaacgtaacc acaactgctg agggtatcgg ctttgcaggt cagccctttt gcatacagcg
     8101 tgacggtatg ctgatggcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt
     8161 tcatcagcag cggaaaggcc tggtccaggt ggtacgcatc tgcatcgctg aacaggcctc
     8221 tgataccggc gcggtcggca aggtaatgca accggttacc ctcctgcacc agacgtgccc
     8281 cgaaacaggg cgtcacggtg cagggcagcc cccaccaggg gcggtcgtga ttgtcgtcgg
     8341 gaagtgttgt cccggggagt gtgtctgaca cgataaaatc cttacagaaa atcggctaag
     8401 aatggtccgg tagtggcgat aattctgctc atcagaattc ccactcagtt cagggtgacg
     8461 ctcatcagcc ggacatacgg accaaaactg tccttacggc gttcagcaaa cacggccagc
     8521 acaccgggaa tatcctgcac ttcacgaccg gtatacgctt cagcactgcc gtgccagcgg
     8581 tatttaccgg tacagaacgg aaaaagacgg gatgtcggat gttgttggtg aatacgcatg
     8641 gcttcaccac gggtgataat tttcataatg ggatacctct gaagacagaa gataaaagtg
     8701 aaaacaggtg tgatgtggtt gtgaagggtt aaagcagacc atgttcggca aaggagaaaa
     8761 cctggttgcc accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct
     8821 gtaccagacg ttcggtgata agccggtctg ccttactggg tgtgacttca ccggacgggt
     8881 gattgtgtgc cagtaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc
     8941 ggggatggac ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct
     9001 gattctggtt gttcagatac agcacccgga attcttcacg ctccagtccc gccatgttca
     9061 gaatcagcca ttcccgtgct gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat
     9121 ggcggtccag ggttttcagg gcccgttgaa tgaggctgcg ctcgccgggc gtcatctctc
     9181 cgggcagaaa ggaaagttgt tgcattgtgc ttctctccat tcagtcgatg atgcgcataa
     9241 tggcgctgca ttccggatgc tgcagggcgt aatcccgcaa ccggtaataa tggaccgtca
     9301 tggcataatt ctccgtacga caggcatgat gactgtacgt catcagacag gcggcaatac
     9361 cggcggcttc cgggctcatt tctgcgcggt taccgttcat ggcattgaac agtacccatg
     9421 tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt
     9481 acagattcca gataccaccg cagtagtctt cgcacagacg gtccatccag ccgaagacac
     9541 ggggctccag ggtcacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc
     9601 gctgttcatc agggacgagg ctggcaacca actgaggcgg attattctct ggtgttgtag
     9661 ctgaataaat tgtgggggta ttctgagaaa cggttttcat gagataactc cttaaataac
     9721 aaataaatgt gtgtttttca ggaacgaaag ctgaatgatt ccctcactca gggaaacggc
     9781 atcagcgcag gctctccagc a
//
LOCUS       DAZUGK010000169         9795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZUGK010000169 DAZUGK010000000
VERSION     DAZUGK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.170"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..189)
                     /locus_tag="ACHY5W_003660"
     tRNA            complement(114..189)
                     /locus_tag="ACHY5W_003660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Lys,seq:ttt)
     gene            complement(193..268)
                     /locus_tag="ACHY5W_003661"
     tRNA            complement(193..268)
                     /locus_tag="ACHY5W_003661"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Val,seq:tac)
     gene            complement(320..395)
                     /locus_tag="ACHY5W_003662"
     tRNA            complement(320..395)
                     /locus_tag="ACHY5W_003662"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Lys,seq:ttt)
     gene            complement(398..473)
                     /locus_tag="ACHY5W_003663"
     tRNA            complement(398..473)
                     /locus_tag="ACHY5W_003663"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Val,seq:tac)
     gene            complement(509..584)
                     /locus_tag="ACHY5W_003664"
     tRNA            complement(509..584)
                     /locus_tag="ACHY5W_003664"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(549..551),aa:Lys,seq:ttt)
     gene            complement(749..1540)
                     /gene="cpoB"
                     /locus_tag="ACHY5W_003665"
     CDS             complement(749..1540)
                     /gene="cpoB"
                     /locus_tag="ACHY5W_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFS4448480.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1550..2071)
                     /gene="pal"
                     /locus_tag="ACHY5W_003666"
     CDS             complement(1550..2071)
                     /gene="pal"
                     /locus_tag="ACHY5W_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFS4448481.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2106..3398)
                     /gene="tolB"
                     /locus_tag="ACHY5W_003667"
     CDS             complement(2106..3398)
                     /gene="tolB"
                     /locus_tag="ACHY5W_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFS4448482.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3531..4796)
                     /gene="tolA"
                     /locus_tag="ACHY5W_003668"
     CDS             complement(3531..4796)
                     /gene="tolA"
                     /locus_tag="ACHY5W_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFS4448483.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEDAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4861..5289)
                     /gene="tolR"
                     /locus_tag="ACHY5W_003669"
     CDS             complement(4861..5289)
                     /gene="tolR"
                     /locus_tag="ACHY5W_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFS4448484.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5293..5985)
                     /gene="tolQ"
                     /locus_tag="ACHY5W_003670"
     CDS             complement(5293..5985)
                     /gene="tolQ"
                     /locus_tag="ACHY5W_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFS4448485.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5982..6386)
                     /gene="ybgC"
                     /locus_tag="ACHY5W_003671"
     CDS             complement(5982..6386)
                     /gene="ybgC"
                     /locus_tag="ACHY5W_003671"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS4448486.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6536..6829)
                     /gene="ybgE"
                     /locus_tag="ACHY5W_003672"
     CDS             complement(6536..6829)
                     /gene="ybgE"
                     /locus_tag="ACHY5W_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFS4448487.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     DLEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6829..6942)
                     /gene="cydX"
                     /locus_tag="ACHY5W_003673"
     CDS             complement(6829..6942)
                     /gene="cydX"
                     /locus_tag="ACHY5W_003673"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFS4448488.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6957..8096)
                     /gene="cydB"
                     /locus_tag="ACHY5W_003674"
     CDS             complement(6957..8096)
                     /gene="cydB"
                     /locus_tag="ACHY5W_003674"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS4448489.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8112..9680)
                     /gene="cydA"
                     /locus_tag="ACHY5W_003675"
     CDS             complement(8112..9680)
                     /gene="cydA"
                     /locus_tag="ACHY5W_003675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFS4448490.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFTFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2374 a         2685 c         2362 g         2374 t
ORIGIN      
        1 gccaatcttt aagattggtg ggtcgtgcag gatgactcgc ttcgctcgcc ctacgggccg
       61 ttgctggcgc aacgttatcc ttcacgctta acatctgagt ttgatgttaa aattggtggg
      121 tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc
      181 taacgacccg agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga
      241 ggtgctctcc cagctgagct aatcacccga tactgcgctg gatactacaa actacttcca
      301 ccgaacccac cgtaattagt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
      361 aaagtcaact gctctaccaa ctgagctaac gacccggtgg tgggtgatga cgggatcgaa
      421 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccgatacta
      481 cgctggatac tacaactaac taggttagtg gtgggtcgtg caggattcga acctgcgacc
      541 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccactttt tcgttgcttt
      601 cggtttgttt gatatcccgt ggcaacggcg gcatatatta ctgatttcag acttgagcgc
      661 aacaaaaatt tcgatgtaga tcactcaact gcttatgatt cgcacgacac gaccagaaat
      721 aatgcgactt ctggtcgtgt gttatgcatt acatcgcgtt cagacgtttt tgcgcctgtt
      781 tagcgccatc agtaccaggg tatttactga taacctgctg gtatacggct ttcgcttttg
      841 cggtgtcacc tttgtcctgc atgatgacgc cgactttaaa catcgcatct gcagcctttg
      901 gtgacttcgg atagtttttc actaccgaag caaaatagta cgccgcatca tcttttttac
      961 ccttgttgta gtttaactga cccagccaat aattggcgtt tggcaggtaa gttgaatcag
     1021 ggtaattttt gatgaaattc tgaaatgcca ccattgcgtc atcctggcgg gatttatcct
     1081 gcaccagcgc aatagctgca ttgtaatccg tgtttgcatc accgcttttc accggcgcgc
     1141 cagcattcgc agtaccagca tcagctgtcg gcgtcgttga tgccgccgca ccgctttgat
     1201 caccgctggt tgattgcgcc gctgcaccac cgctgctgag gctgtcgatc tgcaacagga
     1261 tctgcttttg ccgctccacg acctgattca gttgatactg attttcctga atctgaccac
     1321 gcagggaatc aatatcggat tgattatcag agagttgttg ctggagttgg gttaaaagct
     1381 ggctgtgagc attagaaata cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc
     1441 cgacactact gattggtgcc tgagcaaaag cggcccaggg ggccgctata ccaaccagta
     1501 acgacagact caatagttga tgtctgaagt tactgctcat gcaattctct tagtaaacca
     1561 gtaccgcacg acggtttttg gagtatgccg cttcgtcatg acccagtact gcaggttttt
     1621 ctttaccgta agaaacgatg gagatctggt ctgcagaaac gcctttaccc tgcaggtaca
     1681 tcttaacggc gttcgcacga cgttcaccca gggagatgtt gtattccgga gtaccacgtt
     1741 cgtccgcgtg accttctacg gtgactttgt aagacgggtt gctacgcagg aagtttgcat
     1801 gtgcatccag catttgagcg aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa
     1861 cgatgttgtt ctgctgcagc tgttgcattt gcagacgagc ctgctcttcg gaagacatgt
     1921 tgccgttgcc gccgttcgca tccataccag tgccggcacc cagcatgcct tcgctgccgt
     1981 cattgctggc gttcttgttg gaagaacatg ccgcaattgc cataacaggc agagcaatca
     2041 tcagcccttt cagcactttg ttcagttgca tttcaatgat tcctttacta ttcaattaat
     2101 tattatcaca gatacggcga ccaggcaggg aatttgacct gtccatcagt tgccggaaga
     2161 cgcgctttga aacgcccatc tgtagaaacc aaattcagca cggatcccat cccctgagaa
     2221 gagctgtaga ttaccatagt gccgttaggt gccagacttg gcgtttcatc caggaacgtg
     2281 gacgacagaa cttgtacgcc tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca
     2341 ccgttggagc tgaccattac cataaattta ccgtcgctgc tgacatccgc atcctggttc
     2401 tgcgaacctt cccaggtaat acgttgtggc gcaccgccgt tgatattcac tttataaacc
     2461 tgtggacgac cggcctggtc agaagtaaat gccaggttct ggctgtccgg gaaccaggtc
     2521 ggttcggtat tgttactgcg accatcagtc acctggcgga tctggccaga agccaaatcc
     2581 attacgtaca ggttcaggct accggttttc gacaaggcga atgccagttt gctgccgtct
     2641 ggcgagaatg caggcgcacc gttgtgacgc gggaatgaag ccacctgacg tacagcgccg
     2701 tttgccagcg tttggataac cagcgcggaa cgaccgcttt cgaaggtcac ataagccagt
     2761 ttagaaccgt ctggtgacca cgccggggac atcagcggct gtggtgaacg gtgaacgaca
     2821 aactggttgt aaccgtcata gtcagatacg cgcagttcat acgggaactg accgccgttg
     2881 gtctgaacaa cgtaggcaat acgggtacgg aacgcacctt taataccggt cagtttttca
     2941 aacacttcat cactggcggt atgaccagca taacgcagcc actgcttgtt cactttgtac
     3001 gagttctgag caagtacagt acccggtgcg ccgccagtgt caacaagttg ataagcaaca
     3061 ttgtaagaac catccggatt cggagtgacc tgaccgacaa ctacagcgtc aatgcccagc
     3121 gcggaccatg cagctggttg tacttcctgc gcactacccg gctgctgtgg cagacgagcg
     3181 cgatctaacg gattaaattt accgctgtta cgcaagtcgg cagcaacgat gccgccaata
     3241 tcttcaggtg ccgcaccagg gcccgcccac tggaaaggaa caactccaat aggacgaccg
     3301 gaatctacac cgctgtcgat cacaatgcgg acttcagcat gcagaactga tgcccacagt
     3361 atgagaaaac caaatgctac tcgtaatgcc tgcttcatca tatctccctt atctggaccg
     3421 atggcccacg ataatttagc agaatgttaa caaactaaaa tacacaaaac taccagaacc
     3481 ccgtggcaac cggtgcctga tgttgaccgt cggaacagtc aacatcgcga ttacggtttg
     3541 aagtccaatg gcgcgttttt gaacacttca tatactgcct ggcttggtgg tttcgggatc
     3601 ttcgcaagtt tagctgctgc caacgcagct tgacaaagtg cgggatcgcc accttcaggt
     3661 ttgatatcca gtaacatacc atcgggtgcc agttttatgc gcagcgtaca ggttttgcct
     3721 gcataggacg atgcgtcata gaacttactt tcaatagcag atttaatctg cccggcatag
     3781 ttattgatat cggcccctga tgcgccattg tttttagtat taccactccc ggcaggcgaa
     3841 gcattgttcc ctttcgcccc tccccccgtt ttcggtgcat tcttaccaga gcttagctca
     3901 ccgaaaatat catctgcctc tgcggcagct tttgctgcag cggctttttc agctgctgct
     3961 tttgcagctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct
     4021 gctgcctttt ctgcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct
     4081 gccgctttct tatcagctgc agccttttca gcagccgctt tcttctcagc ttctgccttg
     4141 gctttttcag cagcttcagt tgccgctttc tttcttgctt cagctgcagc tgcttctgcc
     4201 gcttccgctt tcttcttcag cgccgcagct gctgcctcgg cttttttctg cgcttcggct
     4261 gcggctttgg cggcttctgc ttctgctttt ttcttcgcat ctgcagccgc tttcttcgct
     4321 gcatcttccg cagctttagc atccgcttcg gccttcgctt tagcatctgc cgccgctttc
     4381 gctgccgcct cttccgcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc
     4441 tgctttttct gttcctgagc agctaaccgc tctttctcaa gttgcttcag gcgttcctgt
     4501 tcagccgctt gcttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgc
     4561 tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc
     4621 gcacctgaat caaccatgac agcgtcgatg gacgaaccac cgccgcctcc agctgaagct
     4681 tctatattct catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact
     4741 gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc
     4801 ggtttccaaa aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt
     4861 ttagataggc tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag
     4921 tgctttaatt atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt
     4981 cgggttggcc ttgaaacggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc
     5041 caggcgatct ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac
     5101 tggcggatta tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc
     5161 cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc agcaacagca ccagcagtac
     5221 gtccagcaac ggtacaatgt tgatttcgga cttgagatcg cgacgacctc gtccacgcgc
     5281 tctggccatg gcttacccct tgttgctctc gctaacggta aacgcctggc ggtgcagaat
     5341 cgcggtaaac tcttccataa agttgtcgta attcagttcc agtttgttta cgcgttggtt
     5401 gagacggtta taagccatta ccgccggaat agcggcgaac agaccgattg ctgtagcaat
     5461 caacgcttct gcgatgcctg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag
     5521 ggcgataaag gcgtgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat
     5581 ggagccaacc gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat
     5641 ggaaatacgc atcgcacgcg acgccccttc tactacggct tccggcgcat ggctattggc
     5701 acgatgcagg cgcacaaatt ctttgaaccc gctgtaaaag atttgttccg aaccggtcag
     5761 attatcccgt ttcccctggc tctcttgata gaggcgagag agttcgattc cagaccagaa
     5821 tttatcctca aacgcttcgg cttcgcgcgc cgctgcgtta agaatacggg tccgctggat
     5881 aataatggcc caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag
     5941 aaggctagcc ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg
     6001 acaatagact tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga
     6061 acctctgctt cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa
     6121 gaggtgccac gcattgatgt tatttcagtc tgtatttcga gcatatcgtc aagccgcgca
     6181 ggtgcgtaat attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc
     6241 gcctgttgac tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg
     6301 acgtaactgg cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgaacc
     6361 ggccatcgaa acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct
     6421 tttaaacgct gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga
     6481 gcgtaaaaat ttatgggcct ggcgcggccc ataatttttt aaagagaatt ctgatttaaa
     6541 agaagaagaa aatcagcccg acaatgagaa caatatcggc aagcaatggg caaaaaatcc
     6601 cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg
     6661 cccacatcag caataaacca tgccagattt ccagatcact ggtcttcgcg gcgaaacgtg
     6721 atgggtccca aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca
     6781 gggggcgctt gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc
     6841 ttgaccggct ttgcctgatt cgacgtgctc aagcgccagc gcggtgatta ccccaaacga
     6901 acaggcaaga agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag
     6961 tacagagagt gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag
     7021 taacaccagg cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg
     7081 acgttaagcg tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg
     7141 gtgctggacg gcatcacaaa cgggaacatt gcaataccag cagtcaggat gatgcaggcc
     7201 agcgtcaggg aggagaacac aaacgcccat gcggctttat ccatacgtgc agtcaggatg
     7261 gtcagcagcg gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg
     7321 aagttaacca gccatgcgcc agcttcacga accacttctt tattcagcgg gttagaggct
     7381 gcgtaatggt ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg
     7441 ccagccagtg cgaaacagac cagtgtcacc agtgccgcca cctgagccgt tgcacgggta
     7501 cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc
     7561 atccctacgc tcaccacgcc tgccagcagg ccgaacgggt taagcagctg gaagaagtta
     7621 ccggtgtagt acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta
     7681 ccgaacgcta caccaatcac caacggcgga acgaagctac caatgaagat gccccagtcc
     7741 cacatgttac gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg
     7801 aagaacaaag acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca
     7861 tagaccatcg gccaggcagc aaacagtgcg ccgcctgcgg tgatcagcca aacctggtta
     7921 ccgtcccagt gtggtgcgat ggagttaatc ataattcgac gctcggtgtc gttacgaccg
     7981 aggaaacggg tgagcatgcc cacccccatg tcgaaaccat cagtgactgc aaaaccaatc
     8041 agcagaacgc caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga
     8101 cgactcctgt cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga
     8161 ccggttttca ggctgcttgg gccgaggcgt gcaaacttga acattaagaa caattctgcc
     8221 accaggaaca gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg
     8281 gtcagtgacg agttcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg
     8341 ccatattcag ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac
     8401 agcgcggcgc gcagcagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa
     8461 gagagcgcga tgattgccag cagcaggaag ccacacgcca ccatgatacg gaacgtgaag
     8521 tacagcggcg ctacacgcgg aatggagtct ttggttgcct gttgaatctg cgcttcagtc
     8581 gcatcagcca cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtccttcttc
     8641 atgctattga actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgctcgagc
     8701 agagagtacg ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagttctttc
     8761 aggccgataa ctggagtatc cacggaacgc gttgcaatga tgcccagcgc gtaagggatc
     8821 tggatcgcaa atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca
     8881 gcaggtgcag gttgcgtttc ccactcagct tcaatagcag ccagtttggt tttctgcacg
     8941 tcgcccattt cgtagccgga ttcatcaccc agaacaataa cagacagaac ggcagccata
     9001 ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc
     9061 atataccatg cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca
     9121 gtgtgaacga atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc
     9181 tccatacgca tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc
     9241 gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac
     9301 atatgctgaa ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat
     9361 tcgaggaaga aggccatcag accttcgatt gccagcggcg caccgaaaat atcccctaca
     9421 tagtgggaat agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc
     9481 acccccagag cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa
     9541 atctgtttgc cggaaaggac gtagaccgtt tccataatgg ccagcaggaa cgccataccg
     9601 agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac
     9661 agttcgacta tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa
     9721 ccccgttaga aagggtcaca cgcatgcccc aatacaaaat tatttgctcg cctctatcgt
     9781 tgccatttca tcaaa
//
LOCUS       DAZUGK010000170         9745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZUGK010000170 DAZUGK010000000
VERSION     DAZUGK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.171"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..1539
                     /gene="gltP"
                     /locus_tag="ACHY5W_003676"
     CDS             226..1539
                     /gene="gltP"
                     /locus_tag="ACHY5W_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFS4448491.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1729..2418)
                     /gene="yjcO"
                     /locus_tag="ACHY5W_003677"
     CDS             complement(1729..2418)
                     /gene="yjcO"
                     /locus_tag="ACHY5W_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFS4448492.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2512..4659)
                     /gene="fdhF"
                     /locus_tag="ACHY5W_003678"
     CDS             complement(2512..4659)
                     /gene="fdhF"
                     /locus_tag="ACHY5W_003678"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4240..4242),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFS4448493.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4857..6323)
                     /gene="mdtP"
                     /locus_tag="ACHY5W_003679"
     CDS             complement(4857..6323)
                     /gene="mdtP"
                     /locus_tag="ACHY5W_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFS4448494.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(6320..8371)
                     /gene="mdtO"
                     /locus_tag="ACHY5W_003680"
     CDS             complement(6320..8371)
                     /gene="mdtO"
                     /locus_tag="ACHY5W_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFS4448495.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIELRQKL
                     ASEINKLQHSITEGQCWQSDWQISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEATALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            complement(8371..9402)
                     /gene="mdtN"
                     /locus_tag="ACHY5W_003681"
     CDS             complement(8371..9402)
                     /gene="mdtN"
                     /locus_tag="ACHY5W_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFS4448496.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9421..9696)
                     /gene="ytcA"
                     /locus_tag="ACHY5W_003682"
     CDS             complement(9421..9696)
                     /gene="ytcA"
                     /locus_tag="ACHY5W_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFS4448497.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2190 a         2594 c         2617 g         2344 t
ORIGIN      
        1 taacaaatgg ttattcatta atcctgccat aaactcgcgt tattatgcat taatgcagcg
       61 aaaagctctg ttgttaaagg gttgcgcaac ataccgcgca aatgatactg atcataagcg
      121 ttaaaaaaat ctacaaacca acgcaacaca attcatgccc tggcagtatg tcacgttctc
      181 gcgtttctga acggggaacg gcgctccatt gaggaagtta ttctgatgaa aaatataaaa
      241 ttcagcctgg cctggcagat tctgtttgct atggtgctgg gcattctcct gggaagctac
      301 ctgcactacc atagcgacag ccgcgactgg ctggtcgtca atttgctctc tccggcgggt
      361 gatatcttca tccatctgat caaaatgatt gttgtgccga ttgtgatctc cacgctggtg
      421 gtgggtatcg cgggtgttgg tgatgccaaa cagctcgggc gtattggcgc gaaaaccatt
      481 atctacttcg aggtgatcac caccgtcgcc atcattttgg ggatcactct ggcgaacgtc
      541 ttccagcccg gtgccggggt ggatatgtcg cagttggcga ccgtcgatat ctcgaaatat
      601 cagagcacta cggaagcggt acaaagcagt tcccacggca ttatgggcac gattttgtcg
      661 ctggtgccga cgaacattgt ggcgtcgatg gcgaaaggcg aaatgctgcc gatcatcttc
      721 ttctcggtgc tgtttggtct ggggctttcc tccctgcccg cgacgcatcg tgaaccgctg
      781 gtgaccgtgt tccgctccat ctctgaaacc atgtttaaag tgactcacat ggtgatgcgt
      841 tatgcgccgg tgggtgtgtt tgcgctgatt gcggtgacgg tggctaactt tggtttctct
      901 tctctgtggc cactggcgaa actggtgctg ctggtgcatt tcgccattct gttcttcgcg
      961 ctggtagtgc tgggaattgt ggcgcgcctg tgcgggttaa gcgtctggat cctgattcgt
     1021 attctgaaag atgagctgat tctagcgtac tccactgcca gctctgaaag cgtactgccg
     1081 cgaattattg agaagatgga agcctacgga gcaccggcgt cgatcaccag cttcgtggtg
     1141 ccgaccggtt actcttttaa ccttgatggt tcgacgctgt atcaaagtat tgccgctatt
     1201 ttcatcgctc aactgtatgg cattgacctg tccatctggc aggaaatcat tctggtgctg
     1261 acgctgatgg tgacctcgaa agggattgct ggcgtgccgg gcgtgtcgtt tgtggtgttg
     1321 ctggcaacgc tgggtagcgt aggtattccg ctggaaggtc tggcgtttat tgccggtgtt
     1381 gaccgtatcc tcgatatggc gcgtactgcg ctgaacgtgg tgggtaatgc gctggcggtg
     1441 ctggtgattg ccaagtggga acacaaattt gaccgtaaga aagcgctggc ttatgagcgt
     1501 gaagtgctgg gcaaatttga taaaactgcg gatcagtaat tgaagattgc cggggttatc
     1561 ccccggcaat gtgtggatgc ctgatgcgac gctggtgcgt cttatcaggc ctacgtcaga
     1621 cagcgcaata gcctgattta gcgtgatttt gtaggtcgga taaggcgttt acgccgcatc
     1681 cgacatcaac gcctgatgcg acgcttaacg cgtcttatcg ggcctatctt aaccgttggt
     1741 taatttttca aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca
     1801 gtgcagcgcc ttttgcttgt tcttctcgat aaaaccttct tcaccgttta agaacatcat
     1861 tcccgcccag tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacca
     1921 ggttgccttg tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag
     1981 catttgggca tcgacggcag agtcgttctc cagatcttcc gaggcgtttt ctaacagcga
     2041 aatcgccttt ggataatccg gtttacccgc ttgagtattt accagaatat gcgccagcgt
     2101 tacttcacct tctttactgc ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg
     2161 atagtccaga ctgaccggat tggtgatttt gatctgcgcc aacagcgcgc aggcatcagc
     2221 atcaccgctg tcggcggctt tctgtgccca atattcggct ttgctcaaat cgccggagct
     2281 aaaccagctg tcggcaagaa aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa
     2341 atactggctg ccaggctcgg agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa
     2401 tgcgataatt tttttcattt tgataatacg attgggtagg gagtaaccag tataaagagt
     2461 tcgaatggat aaaaaaacag cctccgaaag gaggctgtag aaaggactgt attacgccag
     2521 tgccgcttcg cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt actgctcggc
     2581 ggcgcgctga tcggcgatcg gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt
     2641 cgtaatcggg cttaagtttt cggtaaccag ctcgttacag gcaccaatcc accactggta
     2701 ggtcatataa atcgcccctt tgttcggacg atcgctgacc tgcgcacggg tgataatttt
     2761 gcctttacgc gagtgcaccc aaaccaatgc ctcatcttca atacccagac gtttggcgtc
     2821 ttcggtattg atttgtgcgt agccaggttc atcagccagc gccgccagtg ccgcacagtt
     2881 accggtcatc gaacggcaag agtagtgacc aacttcacgc accgttgaca gtaccatcgg
     2941 gtactcgtcg gtgagtttgt cgattggcgc tacccagtcg caggtgaaga actgcgccag
     3001 accgtttggg gtatcaaact tctctttaaa cagataagaa gtcccctgat cggcatctga
     3061 agtatcgcgg caaggccact gaatgaagcc cagttcgccc attttctcgt aagtcgcacc
     3121 gtagaaatcc gggcacagat gacgcaactc atcccagatc tcctgggtgt tgttgtagtg
     3181 catcggataa cccatacggg tggcgatttc actgatgatt tgccagtccg ttttcagatc
     3241 ccatttcggt tcaaccgcct tgaagaaacg ctggaagcca cggtcagccg cagtaaacac
     3301 gccttcatgc tcgccccacg acgttgacgg taaaataaca tccgccgccg acgcggtttt
     3361 ggtcataaag atgtcctgaa cgataaccag ttccagatct tcaaaggctt tacgtactgc
     3421 cgacagctcc gcgtcagttt gtagcggatc ttcgcccata atgtacgcgg cacgcacttc
     3481 gccatgcgct gcgcggtgcg gcagctcgct gatgcgatag ccggtatgcg ctggcaggct
     3541 ttccacgccc caggctttgg cgaatttctc gcggttagcc ggatctttca catactggta
     3601 tcccggatac gtatccggca gcgcgcccat atcgcaggca ccctgaacgt tgttctgacc
     3661 acgaaccggg ttaacacccg catgtggctt accgaggtta ccggtcagca tcgcgaggct
     3721 ggtcagagaa cgcacggttt ccacgccctg gtagaactgg gttacaccca tgccccacag
     3781 gatggcggcg cttttcgcct gggcatacat ccgtgccgcc tgacgaatct cactggcgct
     3841 gacgccggtg atatcttcaa ccgactccgg cgtgtagcct tcaacgattt tacgatactc
     3901 ttcaaagcct tctgtacggg aagcgacgaa tgctttgtcg tacagatttt cttcaataat
     3961 gacatggccc atcgcattca acagcgcgat gttcgagccg tttttcagtg caatgtgcat
     4021 gtcagcaatg cgcgcggttt caattttgcg cggatcgcag acgataattt tcgccccgtt
     4081 acgtttagcg ttaattacgt gattcgccac gattgggtgg gaatccgccg ggttgtaccc
     4141 gaaaacgaac actaaatcgg tattatcaat ttcgttaata gcattgctca ttgcgccatt
     4201 accgaccgat tggtgcagac ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc
     4261 aacgttattg gtaccaataa cggcgcgcgc aaatttttgc attacatagt tggtttcgtt
     4321 acccgtacca cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact tctctttgat
     4381 ggcgctcagg cgctcggcaa cgtaattcag tgcctcatcc caggaaacag gttcgagttt
     4441 gccgccacgc tggcgacgga tcatgggggt tttcaggcgc ggggtcagga tctgggtatc
     4501 gttaatgaaa tcccagccat aataaccctt cagacacagg gtaccctggt tggttttccc
     4561 ctgcgctgcc tccgcccgga cgattttgcc gttatcgacg accaggttga ttttgcaacc
     4621 tgatgcgcaa taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc cagttaatca
     4681 aatcacgcat acgcgctctc gactacagta tgcatctttt atgccacatt ttatttgggg
     4741 tcattccctg atattacggg cactatttat tcaaaactct gacgaaaaac aggctgtcgt
     4801 cagttttgac gtgacgaaac gaaataccgc gtgacagcca tcacgcggca gacattttat
     4861 tttttctcga cgacgggacc tgcctgatac ccgccgccca gcgatttcat caactgaatg
     4921 ctttggatca cccggcggct gtccagcatc agtaatgaca tctcttcggc aagtactggc
     4981 aaccgggctt cagtggcctg taagcggctg gttaagccgc gctgataggc ggcctcggca
     5041 gcgcgctggg tgaagcgcgt ggcttccacg cgttcagcct gcatctctcg ctcgtcgttg
     5101 agcgtttgca gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag tactgactgg
     5161 ttgtaacgtt caatcatcat gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac
     5221 cgtccaccgt caaacagcgg caatttcaga cccgggatga agttgaactg gcgactggtt
     5281 tttttgaata aggtatccag gtggatggag tccagaccga aaaacgcttt gatatcaaag
     5341 ctcgggtaga acagcgcccg cgcggaatcc acctgatcta atgacgcctg aacataccag
     5401 cgcatggctt gcagatccgg gcgtctggcg agcaactcat aagagagtgt cgccggaatg
     5461 ccggtctgga ctcgtggtaa tgccacaggt ttgatctccg gcatatcgct ggctcccgcg
     5521 ccaatcaatg cacgcagaga ttctcgcgtt tcggtgattt gccctttgac ggcagcaatt
     5581 tgtttatcga ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg cgcttccaga
     5641 ccgtgcgcca ctttactttg gtgcgctttc accgcgtaat caatcacatc gcgagtttgt
     5701 tctaacagat cgagcatctg atagctggcc tgcatactgt aataaagctg cgctacgccc
     5761 gtggtcagcg atagctctac tgctgcggtt tctgccagcg cggcattatg cgcgccaatg
     5821 gcggcggcaa ccgctgagcg atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa
     5881 ccgactgtgg cttccgtata gtacggcccg tccatcccca gtgctggcgc atccattgca
     5941 taagggctta aaaagccgtt cgccgagaca cgttggcggt tgagcatccc taacgctgcc
     6001 acctgcaact gtgaaccggc atctaacaaa tcggcctggg actgcgcttt ttcttcccgc
     6061 agtttcgctt ctgcgagggt gtgtgaaccg cttagcgtcc gctggatcag ggcatccaac
     6121 tgcgggtcat tgagttgttt ccaccactgc gcctgcggcc agccggagct ggcaagatga
     6181 atatcatcgg ccagtttgat ttgttccggt ttgagctgtt gatgaggcgc agaatcctta
     6241 cgtaccaggg cacagccgga aatcagcgtc gtgctgccga gaatgctgca caacagcaga
     6301 cgtgaaagtt gacgattgat cattgcgtgg ctccttgtac ctgttccgtg gcgggtgtta
     6361 atgccggggc tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt
     6421 ttaataaggt gggcaggatg gcctgagagg cgggtgtctc ttcaagcgtt atttgaggag
     6481 aacggctgag tgcggttgcc agaccggcag cgtatttttc cagggcgtcg gcaaactgac
     6541 ctgctctgtc cggctgtagt gcgttatcca gcgcctgcgc cgagttccag gtggcatccc
     6601 aggcgtgaag aagatcgcgg ccctgacgaa taaccgtttg cgaacgttca atcagtaagg
     6661 cgcgttcttc gctgtccagt tgacgctcca gcgccacgcg ttggcacatc tcttcacagg
     6721 cattaaacgc cgcatgcaga cctatacgaa tttgcagata agtgcgcaga gccgtggctt
     6781 cctgctggcg tgggatccgc attaccttac ttaacatacc cagcgcgcca gccagttttt
     6841 gcggcagcgt gcgcgcttca ctttcaggcc agacaaaggt gtaaatcacc gcagacacca
     6901 cggtaccaat gatgataccc agggcgcgat cgcgaatttc caccaggtcg tacaccggac
     6961 caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg ccgatataag
     7021 aagagcgttc agagctggtg gcaatccatg cgcccaacag gaaaatcggt gccagcacaa
     7081 acagcaattc gacaatattg tccagccagg gcatgaccag tagcgtgaat aacagcgcca
     7141 gaatcgcgcc gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac gacgaaccga
     7201 catttggatt agcgacgatc acgcaggtca gcatacaggt atgaatgcct tcccagtcca
     7261 cgccgctgta gaaggtgtaa cagatcaaac aggcgagcag cgtttttacc gcgtagcgca
     7321 tatagtctgg attggtaaaa gcatcggcga ccattgatgg cggtttggcg gcgggcgttg
     7381 gcggcgtatt cgggtccatc tgaccaagct gtaacagcgt ctggcagata ttctccaggt
     7441 tacattcccg tgccgccatc gcttcacttt cgctgatctg ccagtcgctt tgccagcact
     7501 ggccttcggt aattgaatgt tgcagcttgt tgatttctga agctaatttt tgtcggagtt
     7561 caataattgc ctgagaatca gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa
     7621 tgtaggttac cgttgccacg cagctttgcc accatgcgct ttgagttcgc cagttggcat
     7681 cgtccgcgag gcaaaagaca ttgagttttt gcagcgccag cgcctctctt tcaatccgcg
     7741 tttcgggtag cggtgcgagg ctgtccgtca ggtggctaat ggcatcatca agccgatcat
     7801 taagcgcctg atgcatttgc gaaatggcac gactgggaaa ccacagcacg ccgattaacg
     7861 tcatcagcaa ggttggatag aggccaacaa cgatacacca cagcgttaag cgcacgacca
     7921 cttctggata gtcgagcatg gcggggaagg tttgcccgta aatagcgaca atggcgacgg
     7981 cgaaaaagac cagccccaag cgatgggtgc gcatcaaaaa catgcagccc atcaggatcg
     8041 gtccggcgat gatcaatcgg atcaacggtt cgccgtatga ccatttgtag atcaaaaaca
     8101 ggctgccgat ctccagcacc gtggcaacca caaacaagat cgcgacaaat ttggtgtaaa
     8161 acgcgttcga ctgaataccg taaaacagca ctgccagcga taatgccaca aaagggatct
     8221 caaaagtcat cgagatcaga atcaccagca ggcagcctac ccagagttgc acggtctgcg
     8281 gcacgcggcc tggtcgccgc tcgcttaact cttcatgaaa gaacgccagc agcctgacca
     8341 ccggtaatgg cagggagttg agcgcgctca ttattgcggc tcaagattag cgactgccga
     8401 agcgccgatg cggaacattt ccgggtcagg tttatcgacc atgattttga ccggaaaacg
     8461 ctgggcaacg cggacccagt taatagagcg agaaactttt ggcaggccgc ccagcaccag
     8521 gccgccgtca tccggtagta cgccgtagcc aatcgaatcc actttaccct cgaaggtttt
     8581 gccgctgtca ctcatcaggc gaatcgttgc gggtgtacct gagcgaatat ttttcagatc
     8641 ggtttcgcgg aagttggcga tcacatacca gtgacgagtg tcgattaggg taaaaatagg
     8701 gcgcatggca gaagcaaatt gcccgacgga ggttttgagg gaaatgaccc ggccatcaaa
     8761 cggcgcgcga acggtcgcca tttccagatg cagtttggtc agggcaatat ccgcttcgac
     8821 cgccgcacgc tgggcaacta atgcatccac gccgctgacg gcgctggcag ctgactgcgc
     8881 ctgcaacaat acggcattaa gatcggcttc tgcggcgcgc tgcgcagttc ttgcacggtc
     8941 aacgtcttcc gctgagacaa aaccttcttt cagtaatggc tcggtgcggc gtaatgtatc
     9001 tgtggcctgt ttcgcggcgg cacgggcttt ttctaccgtg gcattaaccg agtcggcacc
     9061 aaactgttgc gcgtcaacgc tacgctgggt gagcataatt tgtttatcca gcgccgcgag
     9121 ggaggcttcg gctttcgcca ggttggcttc gtacgggcgc gggtcgatgc ggaacagcaa
     9181 atcgccctgt ttcaccgcct ggttgtcggt gaccgccagt tctacaatgc gaccgctgac
     9241 ttccggcacc acatcaatgg tatctgctga cgcgtaagcg tcattagttg atggcgcact
     9301 gtctacgcgc cagataacga aaacaagggc aaccagcgcc aacgcaacca ctaacagagc
     9361 agggaattta ctgcgaggag cttttttcgg cgtactttcc atgagcgcgt ctcaaataga
     9421 ttagaaaaat gccagccaga acagcatggc gtagagagca aaaagggcgg tataaataat
     9481 tccgacaaat gccagcttga tatttgcccg ctgaataact cgcctcgtga tgagcgtcaa
     9541 tataaggctg gcaatggcgc agaaaaacca gctgggataa taagcgccga tcaccgggat
     9601 tgccggagat aatgagcatc ccgaaaccat gaagacggga attatccagg ctgttttttt
     9661 gagttgcatc gccatgcgag agagaactgt tggcatatta aatacatcct gtctgctgcg
     9721 aacaatatct gtagtgaata tcaat
//
LOCUS       DAZUGK010000171         9708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZUGK010000171 DAZUGK010000000
VERSION     DAZUGK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.172"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..707
                     /locus_tag="ACHY5W_003683"
     CDS             24..707
                     /locus_tag="ACHY5W_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS4448498.1"
                     /translation="MNLKKILLSVLMISPLCMLTGCDYIGKAKLIDDLSKQQELQKSK
                     IEQLEKQQEQDKRKIELFEKQQTAIISSTKTLASVVKGVKEKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTALNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            751..5625
                     /locus_tag="ACHY5W_003684"
     CDS             751..5625
                     /locus_tag="ACHY5W_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS4448499.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALATGNEPGTPVGTGSGWVAIGQDSQANAFTDGNGASTAVGYKAFAEGTWSS
                     AIGAMTHAIGPASMAFGVNAVSTGQRSIAMGASSSSVGQFSMALGRYANSGGDYSIAQ
                     GDKSNAAGLNAIAVGRSSSAAGEKAIALGNAAKATEIMSIALGDTANASKAYSMALGA
                     SSVASEENAIALGRSSVASGTDSLAFGRQSLASAANAIAMGAETEAAENATAIGNNAK
                     AKGTNSMAIGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSR
                     GLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKI
                     VNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGSAVDVDDGTVTAPTYNLKNGSKN
                     NVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQL
                     KATNDDVEANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLKDDALLWNDAKNA
                     FSAAHDKDTTSKITNVKDGDLTAGSTDAVNGSQLKTTNDAVATNTTNIATNTSNIATN
                     TTNISTLTETVTNLGEDALKWDKDSGVFTAAHGTEATSKITNVKDADLTADSTDAVNG
                     SQLKTTNDAVATNTTNIANNTSNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNAAS
                     KITNILDGAVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNY
                     YNVGDALAAINSSFSTSLGDALLWDASVDKFSAKHGTNGDASVITDVANGDVSSTSSD
                     AVNGSQLYTTNKYVVDALGGNAEINADGSITAPTYTIANADYDNVGDALNAIDTTLDD
                     ALLWDADAGENGAFSAAHGKDKTASVITNVANGAISATSSDAVNGSQLYTTNQYIVDA
                     LGGDAEVNADGTITAPTYTIANTDYNNVGDALDALDDNALLWDETANGGAGAYNASHD
                     GKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNSQIINQLAGNTDATYIEENGA
                     GINYVRTNDNGLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSS
                     SVSSRVIAKGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHD
                     AVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLN
                     TLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSV
                     GSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNI
                     ENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEE
                     NTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLG
                     GGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSG
                     GIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNS
                     QGEYSAALGAGIQW"
     gene            6051..7706
                     /gene="lldP"
                     /locus_tag="ACHY5W_003685"
     CDS             6051..7706
                     /gene="lldP"
                     /locus_tag="ACHY5W_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFS4448500.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7706..8482
                     /gene="lldR"
                     /locus_tag="ACHY5W_003686"
     CDS             7706..8482
                     /gene="lldR"
                     /locus_tag="ACHY5W_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFS4448501.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8479..9669
                     /gene="lldD"
                     /locus_tag="ACHY5W_003687"
     CDS             8479..9669
                     /gene="lldD"
                     /locus_tag="ACHY5W_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFS4448502.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2435 a         2443 c         2547 g         2283 t
ORIGIN      
        1 gattatgaaa aggaattcat cctatgaatc taaaaaagat actattaagc gtgttaatga
       61 tatcgccact ctgcatgttg acagggtgtg actatattgg aaaagcaaaa ctaatcgacg
      121 atctttccaa acagcaagag ttgcaaaaaa gtaaaataga gcaacttgaa aaacaacaag
      181 aacaagataa acgcaagata gaactatttg aaaaacagca aactgccatc ataagtagta
      241 ccaaaacgct tgctagtgtg gtgaaggggg ttaaagaaaa gcaggatgaa tttgtcttca
      301 cagaatttaa cccagcacaa acacaatact tcattttaaa taacggctct gtcggtttgg
      361 cgggtcgggt gctggctatt gatgcagttg aaaacggcag cgttattcgt atttcgctgg
      421 tcaacctatt aagcgttccc gtatcaaata ttggttttca tgctacatgg ggcaatgaaa
      481 ggcccaccga tgccaaagct ctggcgaagt ggcaacaatt acttttcaat accgctctga
      541 actccactct gcaattaatg cctggtcaat ggcaagacat taatctgaca ttaaaaggcg
      601 tttcgcctaa taacctgaag tatctgaagt tgtccatcaa tatggccaat ctccagttcg
      661 acactgttca atctgctgaa actcggcagc gaaaaaacaa aaaataaatg cagcttctgc
      721 gcttatatca aggaatagag aacatcaata atgaacaaaa tatttaaagt catctggaat
      781 ccggcgacgg gcagttatag cgttgccagt gaaacagcaa agagccgtgg taagaaatct
      841 ggtcgcagta agctgttaat ttctgcccta gttgcgggtg gaatgttgtc gtcgtttgga
      901 gcgctggcaa caggtaatga acctggtaca cctgttggca caggatctgg ctgggttgct
      961 ataggccagg attcacaggc aaatgctttt acagatggta atggtgccag tactgcagtg
     1021 ggttataaag ctttcgctga aggaacctgg agttccgcta ttggcgccat gacccatgca
     1081 attggtccgg cttcaatggc attcggggtt aatgccgtct caaccggtca acgaagtatc
     1141 gctatgggcg catcgtcaag ttcagtgggt caattttcga tggcacttgg gcgatacgcg
     1201 aattcgggtg gtgattattc gatcgcgcaa ggggataaat ccaatgctgc tggtctgaat
     1261 gccattgcag tgggaagaag cagtagtgcg gcaggcgaga aagccattgc gctgggcaac
     1321 gcagctaaag ctactgagat tatgagtatt gctcttggcg acaccgccaa tgcgtcaaaa
     1381 gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc aattgccctg
     1441 gggcgtagca gtgtagctag cggtactgac agcctcgcat ttgggagaca atcacttgcc
     1501 agtgcagcaa acgctattgc gatgggtgct gagaccgagg ccgctgaaaa tgcaactgct
     1561 attggcaata atgcgaaggc aaaagggact aatagcatgg caatagggtt cggaagcctt
     1621 gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca
     1681 atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt
     1741 agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat
     1801 aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc
     1861 gcagattcca ttgcggattt agacaatacc gtctctgttg gcaatagttc attaaaacgc
     1921 aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt
     1981 tcacagcttt atgccattag cgactcggta gcaaaaaggc ttggaggagg gtctgcagta
     2041 gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg tagcaaaaat
     2101 aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa
     2161 ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat caccgatgtt
     2221 gcggatggca cgatttcagc ctccagtaag gatgccgtta acggttccca actgaaagct
     2281 accaatgacg atgtcgaggc caataccgcc aatatcgcta ctaataccag caacattgcc
     2341 acgaatacgg caagtattgc caccaacacc actaatatca ccaacctgac ggattccgtt
     2401 ggtgacctta aggatgatgc cctgctgtgg aatgacgcta aaaatgcatt cagtgcagct
     2461 catgacaagg atacaaccag caaaatcacc aacgttaaag atggcgacct gacggctggc
     2521 agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat
     2581 accaccaata tcgccactaa cacttccaat attgccacta acaccaccaa catctctacc
     2641 ctgactgaga cggtgactaa tcttggtgag gatgcgctga agtgggataa ggacagtggt
     2701 gtcttcacgg cagcacacgg caccgaggcc accagcaaaa tcaccaacgt taaagatgcc
     2761 gacctgacgg ctgacagcac cgatgccgtt aacggctctc agctgaaaac caccaacgat
     2821 gccgtggcga cgaataccac caatatcgcc aataacactt ccaacatctc taacctgact
     2881 gagacggtga ctaaccttgg tgaagatgcg ctgaaatggg ataaggacaa tggtgtcttc
     2941 acggctgctc atggcaacaa cgccgccagc aaaatcacca atatcctgga cggcgcagtc
     3001 actgcaacca gttccgatgc cattaacggc agccagcttt atgacttaag cagcaatatc
     3061 gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc
     3121 tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc
     3181 tcatttagca cgtctctcgg cgatgccctg ctttgggacg cttccgtaga taaatttagt
     3241 gccaaacacg gtactaatgg tgatgcaagc gtgatcactg atgtcgcaaa cggggatgtt
     3301 tcaagcacaa gctctgatgc tgtgaacggc tcgcaactct ataccaccaa taagtacgtc
     3361 gtagatgcgc tgggtggtaa cgccgaaatc aacgctgacg gcagcatcac tgcgccgacg
     3421 tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc
     3481 actcttgatg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc
     3541 gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct
     3601 gccaccagca gcgatgctgt gaacggctca caactctata ctaccaatca gtacattgtc
     3661 gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac
     3721 accattgcga ataccgatta caacaacgtc ggtgacgccc tggatgcgct tgatgataac
     3781 gccctgctgt gggatgagac tgccaatggc ggtgcaggag cctacaatgc cagccatgac
     3841 ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga cagtaccgat
     3901 gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cagccaaatc
     3961 atcaatcagc ttgctggtaa taccgacgca acctatatcg aagaaaatgg cgctggtatc
     4021 aactacgttc gtactaacga caacggttta gcctttaacg atgcaagcgc ctctggtgtc
     4081 ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg ccagcagcgt agccattggt
     4141 cagaacagca gcagcaccgt tgataccggt attgcgctgg gtagcagctc cgtttccagc
     4201 cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg aaaatggcgt ggtgattggt
     4261 tatgacacca ctgacggcga actgctaggt gcattgtcaa ttggtgatga cggtaaatac
     4321 cgccaaatca tcaacgtagc tgatggttca gaagcccatg acgccgttac ggttcgccag
     4381 ttgcagaacg ctattggagc ggtcgccact acgccaacca agtactttca cgccaactca
     4441 acggcagaag actcactggc cgttggtgaa gactcgctgg caatgggtgc gaaaactgtc
     4501 gttaatggta atgcaggtat tggtatcggt ttgaacactc tggttctggc tgatgcgatc
     4561 aacggtattg ctatcggcag taacgcacgc gcaaaccatg caaacagtat tgcaatgggt
     4621 aacggttctc agaccacccg tggtgcacag actggctaca ccgcctacaa catggacgca
     4681 ccgcagaact ctgtaggtga gttttctgtt ggtagtgaag acggtcaacg tcagatcaca
     4741 aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt gaaagtcact
     4801 gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca ggtcactaat
     4861 ctggatactc gcgttactaa tatcgaaaac ggtattggcg atatcgtgac caccggtagc
     4921 accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg taaagatagc
     4981 gttgcgattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact gggtacaggc
     5041 tccgtggcca acgaagaaaa cacgatctct gtgggttctt ctaccaacca gcgccgtatc
     5101 accaacgtag ctgccggtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct
     5161 tctgaagcag gcggcgttcg ctacgacacc aaagctgatg gctctatcga ctacagcaac
     5221 atcactcttg gtggtggcaa cggaggtacg actcgcatca gcaacgtttc cgcaggtgtg
     5281 aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgtgcagga aacaaagcaa
     5341 tacaccgatc agcgcatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg
     5401 agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta cactccaggt
     5461 gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt
     5521 gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc
     5581 cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta
     5641 gtgtgactaa gggcaaacgc caaattgcct gatgcgctac gcttatcagg cctacagggt
     5701 gattctgcac attcctatag gccgggtaag gtgttcacgc cgcatctggc aagataaggc
     5761 gctccgggtc aacaatctaa gggcaattct ctgatgagga ttgccctttt ctttaccaga
     5821 catctccccc acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     5881 ttccccaatt aaaactcaca tcaatgttgc taatacataa catttagtta accattcatt
     5941 gtcattatcc ctacacaaca taattggcag tgccactttt acacaacgtg tgacaaggag
     6001 atgagcaaca gactcattac acgatgtgcg tggactccag gagacctgca atgaatctct
     6061 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagcctgata gcatcgcttc
     6121 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct
     6181 catggacggt ggcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     6241 acgcgctggc ctcggtggtt tatggcttct tctacgggtt gtggcccatc gcgtggatca
     6301 ttattgcagc agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     6361 gctcgtctat tctttcgata accccagacc agcgcctgca aatgctgatc gtcggtttct
     6421 gtttcggcgc gttcctcgaa ggagctgcag gctttggcgc accggtagca attaccgccg
     6481 cattgctggt cggtctgggc tttaaaccac tgtacgccgc cgggctgtgc ctgattgtta
     6541 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaggtaa
     6601 caggtatcga cagctttgag attggtcaga tggtggggcg gcagctaccg tttatgacca
     6661 ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtata aaagagacgt
     6721 ggcctgcggt cgtggttgca ggcggttcgt ttgccatcgc acagtatctc agctccaact
     6781 tcattgggcc ggaactgccg gacattatct cttcgctggt atcgctgctc tgcctgacgc
     6841 tattcctcaa acgctggcag cctgtacgcg ttttccgctt cggcgatctg ggtgcatcgc
     6901 aggttgatat gacgctggct cacaccggtt acacggcggg tcaggtgctg cgtgcctgga
     6961 caccgttcct gttcctgacc gccaccgtaa cactgtggag tatcccgcca tttaaagccc
     7021 tgttcgcttc gggcggcgcg ctttatgaat gggtgatcaa cattccggtg ccgtatctcg
     7081 acaaactggt tgctcgtatg ccgccggtcg tcagcgaagc taccgcttac gccgccgtat
     7141 ttaagtttga ctggttctct gccactggca ccgccattct gtttgctgca ctgctctcaa
     7201 ttgtttggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acactgaaag
     7261 aactggccct gcccatctac tctataggta tggtgctggc gttcgccttt atctctaact
     7321 attccgggct gtcatcaaca ctggcgctgg cactggcgca caccggtcat gcttttacct
     7381 tcttctcacc gttccttggt tggctggggg tattcctgac cgggtcggat acgtcatcta
     7441 acgctctgtt tgctgctctg caagccactg ccgcacaaca aattggcgtc tctgacctgt
     7501 tgctggttgc agccaacacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     7561 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     7621 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt
     7681 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     7741 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     7801 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactgc gcgaggcgct
     7861 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     7921 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     7981 cgatgatccg gattacagtt tcgatattct ggaggcccgc tacgccattg aagccagcac
     8041 cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt
     8101 tgaagcaacg ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     8161 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga
     8221 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     8281 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgccg gtgatgctga
     8341 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     8401 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca
     8461 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     8521 cgcattctgc cgccgttcct gttccactat atggatgggg gtgcatattc tgaatacacg
     8581 ctgcgccgca acgtggaaga tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac
     8641 atgtccgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccagtggcg
     8701 ctaggtccgg taggtttgtg tggcatgtat gcgcgacgcg gcgaagttca ggcagccaaa
     8761 gcggcggatg cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa
     8821 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     8881 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     8941 accgtggata tgccgacacc gggagcgcgt tatcgtgatg cgcattctgg gatgagcggc
     9001 ccgaacgcgg caatgcgccg ctatttgcag gcggtgacgc atccgcaatg ggcgtgggat
     9061 gtgggcctga acggtcgtcc gcatgattta ggaaatattt cggcttacct cggcaaaccg
     9121 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     9181 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     9241 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atgggattgt agtttctaac
     9301 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcactgcc tgctattgca
     9361 gatgcggtga aaggtgatat agccattctg gcggatagcg gaattcgtaa cgggcttgat
     9421 gtcgtgcgta tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcttgtat
     9481 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag
     9541 atgaaagtgg cgatgacgct gactggcgcg aaatcgatca gcgaaattac gcaagattcg
     9601 ctggtgcagg ggctgggtaa agagttgcct gcggcactgg ctcccatggc gaaagggaat
     9661 gcggcatagt catttgcgcc ccctccccct aaccctctcc ctcaggga
//
LOCUS       DAZUGK010000172         9665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZUGK010000172 DAZUGK010000000
VERSION     DAZUGK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.173"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..398
                     /locus_tag="ACHY5W_003688"
                     /pseudo
     CDS             1..398
                     /locus_tag="ACHY5W_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            408..1321
                     /locus_tag="ACHY5W_003689"
                     /pseudo
     CDS             408..1321
                     /locus_tag="ACHY5W_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1998..2216
                     /gene="yiiE"
                     /locus_tag="ACHY5W_003690"
     CDS             1998..2216
                     /gene="yiiE"
                     /locus_tag="ACHY5W_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS4448503.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2433..2675
                     /gene="yiiF"
                     /locus_tag="ACHY5W_003691"
     CDS             2433..2675
                     /gene="yiiF"
                     /locus_tag="ACHY5W_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFS4448504.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(3005..3934)
                     /gene="fdhE"
                     /locus_tag="ACHY5W_003692"
     CDS             complement(3005..3934)
                     /gene="fdhE"
                     /locus_tag="ACHY5W_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFS4448505.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3931..4566)
                     /gene="fdoI"
                     /locus_tag="ACHY5W_003693"
     CDS             complement(3931..4566)
                     /gene="fdoI"
                     /locus_tag="ACHY5W_003693"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS4448506.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4563..5465)
                     /gene="fdoH"
                     /locus_tag="ACHY5W_003694"
     CDS             complement(4563..5465)
                     /gene="fdoH"
                     /locus_tag="ACHY5W_003694"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFS4448507.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5478..8528)
                     /gene="fdnG"
                     /locus_tag="ACHY5W_003695"
     CDS             complement(5478..8528)
                     /gene="fdnG"
                     /locus_tag="ACHY5W_003695"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7941..7943),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS4448508.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVHEDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8722..9555
                     /gene="fdhD"
                     /locus_tag="ACHY5W_003696"
     CDS             8722..9555
                     /gene="fdhD"
                     /locus_tag="ACHY5W_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFS4448509.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGIDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELAGGHEDVGRHVALDKLLGRRAQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2378 a         2352 c         2491 g         2444 t
ORIGIN      
        1 tgaatgcttc gagtattaat caaacttact tacatgataa aaatcctggt gacgttatta
       61 gcctgtatgg cactttagat aacattcccc ttgtagttaa ggaatgcacg ggtaaaaatc
      121 atcaaattaa cgttaaagcc aaattgccac aagaacgaag tgaaaaagtt gaggatatgt
      181 gtgaaagaat gagaggtggc atctctatgt ttaatcacac cacaaaaaca gctggtaata
      241 tagagcataa tttgcagcta agttttctcg tggattcacg gccgtcgatc accaataaat
      301 actcagccaa agatgtagcg ccagacatac ttgtattgcc cgtcaacgta tatcatgcgg
      361 attttaaaat taaaatcaat gacgcagtag agaagacttc gtcaattgtg ttatgtgctg
      421 tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg gataataggt aatgtaccaa
      481 aaaataataa accaaaaaca atcatttttg atttacgttt tcttagtgca attaagcaga
      541 aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca taccgcaatg atgagggagg
      601 catgtaagga attaccgcag ctagtggagt atgttccttt agcctgtgaa gcgcatagcc
      661 atcgcagcgt cgcccttgcc aaaaccgtgg ctgcacccat catggcgaca ggtgtgggcg
      721 cggctttaat gtatgcaaca tcatcaggac cgtattatgc gcaaagtctt tctggttcgc
      781 cggatatcga gatgataaaa aacgcatggc tgaattgttt aaccagtcgg atacttctgt
      841 gcgcaacaaa tatcgcccgg cttttgataa atggaatgaa ctggtagata aattaaatca
      901 cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt ttagcgacag cgataaatga
      961 aacttctggg ggggatacga atgttgccgt tgtgatgggg tgtaaatctg cgaaagatcg
     1021 cacgatatcc attgtccttg gcaactcgat gttgcaaaca ctctttgaaa aacgcctcgc
     1081 cgatagtgga gaaatagaaa atctttttga tcaacaggga tattttagtt gcgattcatt
     1141 aacacctgaa gaagtaatga tgttaaaaga cctttttgat attcgagttc ttcatgtctc
     1201 taacaaattc aatgttgggt tgcaaggtaa tatcaacaca gatgttttac aagattcgtt
     1261 ttttaaaaat gtcgatttca ttcatgaatc taactcgtat accgtaggga tgggagtctg
     1321 acgagtaaaa ctcattagga ttatcagaat tcggacaatg cattgcaaca atgacataca
     1381 aagagagatg tgtgcaggca cttagctgcg aggggcatgg ttatgccata cagaaagcac
     1441 cagcattcag aactccagtg aaagcggcat agtggccgcc tgctcaggag ttttgctatg
     1501 acccgtatcg tgctgagcgt tgccacccta tgatgcgatt aaaaaaactc accgtcgcgc
     1561 tgatgattga cgtcaatgct atcggatgat gccgggggtg ttccggaagg aattccttgt
     1621 ttcccgaact gttaccaaca aaattataac gatgagcaag taaagaccgg ttggtggcag
     1681 tactgaccgg tctgtgatgg aagattaatt tcaccttaaa tcctggtgct gttaggatgc
     1741 tgacgttttt cggctacacc attcagatta tgcaaataaa tatccctgaa tataatatat
     1801 tcatagcacc cataactcat cctgggcgat aactaaacca tttttgagta ataaatgaaa
     1861 caactgcaac gctcttatta tttctggaaa acgtctcgca aaaacaaaac aacatgcaaa
     1921 aatccgcttt cgttaattaa ttttccggcg taattttcgc acatacatta atgagtaata
     1981 tatgtaaggt ggtggtaatg gcgatgaata cggtttttct tcatttatca gaagaggcaa
     2041 ttaaacggct gaacaagctt cgtggctggc gtaaggtttc acgatctgca attttacgcg
     2101 aagcggtaga gcaatatctg gaacgtcagc aattcccggt gcgtaaagca aagggaggca
     2161 ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa ggagcataag gaatgacaaa
     2221 aatttttcat tcttgaatat aaaaaacaga tgcccttatt ctggtattaa tacaaggctg
     2281 ttttacttga acttataata actgcaactg ttacatcata tctggaaaac gcctcgcaaa
     2341 aataaaaaat gatgcgtaaa tgagctttct ttaaagattc gacaagggta cttttctctc
     2401 catagatatc aaatgatatt ggtcaaatat ttatgaactc tctggcaggt attgatatgg
     2461 gcagaattat actcgattta tcgaatgagg tgattaagca acttgatgat cttgaggtgc
     2521 agcgtaatct tcctcgtacg gagctattac gggaagcggt agatcaatac ttggtaaatc
     2581 aatcgcaaac agtaagaacc agtgcttttg gcatatggca agggcgtgag gaagatggtg
     2641 tcgaatacca gcgtaagctg cgcgaggaat ggtaatggga aaaaatgata tcaatcagat
     2701 tgctgacaac gtgtgcgttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
     2761 aagtcgtata aattcaatgt attgcagttt attggtaggc ctaataagcg cggcgcatca
     2821 ggcaatctta cgcttgtcat cagtgacata cccataacct tatcgatgtg gtgatgggta
     2881 aattgaggcc aaacaggcac tggcggcata gcttgaggtg gtaacgcgga atgtgagaga
     2941 tggtgtcgtc acaccctatg agctgtaatc cgggcgacca gcgccacccg gaaaacgtca
     3001 gagattactc cccttcaccc ggaaacagga acgggttgat ggaactgcgg gcatagcctt
     3061 cttgctccat tcgagcgtcc agtaccagag aggcgaggtc atctgccacg gcttcaactt
     3121 tcggttcttt ttcctgatag agaattttca ggtaagtgcc gcagtcatcg cagctttcgg
     3181 ctttaatcgc ggcctgttcg tcatccagcg accagtaatg cagtttgccg ctctgctcac
     3241 agttgctgca ttttacgcgc actacgtgcc attcggtttc acacaggttg cagtgcaggt
     3301 aacgcagacc ctgagtggtg ccaatttgca ccatgctgga caccggcata gagccacata
     3361 ccgggcaata ttgacgttgt tcgccgtatt cagcgcgggc tttgccgggg atcagattgg
     3421 ccatctgcgc ccagtagagc gacagtgcag cccagataaa cggcgcttta tcgctactga
     3481 cggatgagaa atcagaggca aacagtgcgc tggccatatc ttccagctcc tgagtcgatg
     3541 ccttctccag attctcaatc actgccagcg ccgggccgct catttcaggt ttcagctcag
     3601 caattagcgc catcagcagc ttttgccagt gcttatcacg cggcagaacg tgaatatcca
     3661 gcggaggctt gccttgtgcg ctggcttctt taatgcgtgc agtcagatcc atctccagcg
     3721 gatggtcgta cagcaccact tcctgggcgt gggcgataag cgcagcaaag cgcaggtaat
     3781 cacccagcgg attattttct gccagctcgc gcagacgctc ggcgcggcgg ttgtataaat
     3841 tcttgagccg agggaacaat aacggcggaa tcatatccgc cgtacgtttc tcgctcgaac
     3901 ccagctcatc ttgcgggatt atgcgaatac tcattcagct ttcttttccg ttgtcttgcg
     3961 gacctcacgg taccagcgcg ggtgatgttt cttcgcccag gcgctggtaa cccatccttc
     4021 caccatggcg gtaatcgtgc ctttcaccca aagggcggcg tagatatgca ccatgataac
     4081 cacaattaac gccactgcgg caaatgaatg cagcattaac gcgaatcgga tcaccgggat
     4141 tgagaaagca ggcgcaaaat aaggacgcca gataatcacg ccgctcacca gcagcagaac
     4201 caggaaaata atcgccgccc agaaaacgca tttctgaccg aagttataac gcccggtgtc
     4261 acctacttcc tcgttgacga cgatcttacg aatattcttc gcccaaaaga tatcatcccg
     4321 attgattagg ttgtgatgcc agtaacggaa aaacatgatg atgaacgagg caaacataac
     4381 cacgccgaca aacgggtgca gaattcgcgc cagctgcggt gtgcccatga tttgcatcaa
     4441 ccagttgaag gacgggaaca aaaagcccag cccgctcacc gccgccagga tgaagcagaa
     4501 ggcggtgatc cagtggttga tacgttccgg cgcggtgtag cgcacgatgg tgtcacgtcg
     4561 tttcatttgc gctcctcgtc tttctcttcg tgcagattat tctcttcctc atccgcacgg
     4621 ttcggaccga cacccacgta gtggaagata ctggccgcaa aggtagccgc aaagccaaca
     4681 gccgcgagcg gtttccagat gcctttccag aatttcacgg tttcgctgat ttccgggttc
     4741 tccggcaagc catgatacag atttggcttg tcagcatggt gcagcacgta cataacgtgt
     4801 gtaccaccga cgcctgccgg atcgtacaga cccgcattgt cgtaaccgcg ggttttcagc
     4861 tcagcaacgc gctcgctcgc cagcgttttc atcgactctt tcgtaccgaa gtgaatcgcg
     4921 cccgttgggc aggtcttcac gcaggctggt tcttgcccaa ccaccacgcg gtcaacgcac
     4981 agcgtacatt tgtagacgcg gttgtcttcc gggttgaggc gcggaatgtc gaacggacag
     5041 cccgcaatgc aataaccgca gccaatgcac tgctcggact ggaagtcgac gataccgttg
     5101 gcatactgaa taattgcacc ttccgccggg cacgctttca ggcagccagg atcggaacag
     5161 tgcatacagc cgtctttgcg gatcagccat tccagtttgt cgttctgctc cacttccgag
     5221 aagcgcatca ccgtccacga tttggcactt aaatcattgg ggttgtcgta caccccaatg
     5281 ttattgccga cggtatcgcg aatgtcgttc cactctgaac acgccacctg acaggcttta
     5341 cagccgatac aggtggtgac gtcgatgagt ttcgccactt cttcctggaa gtcccgcgcc
     5401 tgaggcgcgg gggtcagacc gttagtcgcg gaacgacgaa tgatatcttg cgattgataa
     5461 gccatatgtc gtctccgtta caccttttcc acattcacaa ggaaggactt aaactccggc
     5521 gtctgcgtgt ttgcatcacc aacgaatggc gttaacgtat tggcaataaa gcctttttta
     5581 gctacgcctt catagcccca gtgaatcgga ataccgatgg tatcgatatc tttgccgttc
     5641 gctttcagcg tgcgaatacg tttggtcacc accgctttgg ctttgatata gccacggttg
     5701 gaggagactt tcacggtatc gccctgggca atgccaagtt tattcgccag cgactccccg
     5761 atttccacaa actgttctgg ttgcaaaatc gcgttcaaca gcgcgtgttt ggtccagtag
     5821 tggaagtgct cggtcagacg ataggtggtt ccgacatacg ggaacttatc ggctttaccc
     5881 aatgcttcgg cgtcgtcttt aaagatacgt gcagccgggt tcgagataac gtttggatgc
     5941 agcgggttag ttcccagcgg cgtttcaaac ggctcgtagt gttccgggaa cggaccttcc
     6001 gccatcttat cgagggcaaa cagacgcccc atgccttcct gctgcatgat aaacggcccg
     6061 acgccgctgc ccggaggcgc tgcgctgtag tccggaatat cccagccggt ccacttagtg
     6121 ccgtcccatt tcagcaactg acgcttcgga tcccacgggt taccctgcgg atctgcggag
     6181 gcgcggttat acagaatgcg gcggttaagc ggccatgccc atgcccagcc cagcgtgtta
     6241 ccgaggccag acggatcggc gttatcacga cgcgccatct ggttgccttc cggcgtccag
     6301 ctaccggcga aaatccagca accacaggag gttgtaccgt catcgcgcag ttgggcgaac
     6361 gagctaagtt gttggccttt cttgacgata accgcaccgg ttgccggatc ggtaatatcg
     6421 gccagcgcct taccgttgct ctccatcgct acttcttccg acttcggctc atgcggaatg
     6481 gcgtagttcc aggtcatgtt cagcacctgg tccgggttcg cgccaccctg ttcggcatac
     6541 atcttgcgca agcgcaggaa gataccggag aggatctcgc cgtcagtcag cgcaatcccc
     6601 ggggcgtccg cacctttcca gtgccactgc aaccagcgac ctgagttaac gattgaaccg
     6661 ttctcttccg cgaagcaggt cgatggcaga cggaacactt cggtctggat cttcgacgag
     6721 tcaacttcgt tcagctcacc gtggttctgc cagaagttag aggtttcagt gttcagcggg
     6781 tcgatggtga cgaggaactt cagtttcgac agacaaccga tcactttgtt tttgttcggg
     6841 aatgaggcaa cagggttaaa gccctggcag atatagccat tgaccttgcc ctctttcatc
     6901 atctcgaagt attgtaggac gtcgtagcct ttatcccact tcggcaacca gtcaaagccc
     6961 cagctatttt ccgccgtcgc tttatcacca aagaaggcct tcatcataga gacgaagaat
     7021 ttcgggtagt tgccccagta gtttacctgg ccttccagca gcggttttgg cgtgttggcg
     7081 gtaaggtagg tttgcagatc ggtctgcttc tcgcttggca gcgtcatgta acctggcagg
     7141 ctctgcgaca gcagccccag gtccgtcagc ccctgaatat tggagtgacc gcgcagggcg
     7201 ttaacgccgc cgccagccat ccccatgtta ccgagcagca gctggatcat cgccatcgta
     7261 cgaatgtttt gcgcaccaac ggagtgttgc gtccagccga gggcatacag gaacgaggca
     7321 gttttatcgt gagcactggt ttctgcgatg tattcgcaga ctttcaggaa cgcgtctttt
     7381 ggcgtaccac agatgttttc aaccacatct ggcgtgtagc gggaaacgtg ctgtttcagc
     7441 aagttccaca cgcagcgcgg atgttgcagc gtggtatcgc gtttggcgaa gccgttttcg
     7501 tccagttcat aagtccagga ggatttatcg tacttgcgtt tttccgcgtc gtagccggtg
     7561 aataggccat cttcaaagcc gtaatcctca tgcacgatca ggctggcgtt ggtataggct
     7621 tcggtgtatt cgcggttgaa tttttcattg ttcagcaggt acagcaatac gcctgacagg
     7681 aaagcaatgt cagtaccgga acgaataggg gcatagtagt cagctaccgc cgccgtacgc
     7741 gtaaagcgag gatcgatcac aatcagcttc gcgccgttgt gaattttggc ttccatcgcc
     7801 cagcggaacc cgaccgggtg agcttcagcg gcgttaccgc ccatcaccac gacgaggttg
     7861 gcgttcttga tgtcgaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga
     7921 cttgctaccg ttggtccgtg tcagacacgc gcctggttgt cgaccgcgag catacccagc
     7981 gcgcgggaga atttttgcgt taaatagccg gtttcgttac tcgacgcgga agcacacagc
     8041 atcccggtgg agagccagcg gttaacagtc acgccttcgg cgttttgcgc aatgtagtta
     8101 gcatcgcggt cttctttcat cagtttggcg atgcgatcaa acgcctcttc ccaactgatt
     8161 tgctgccatt tatcagaacc tggcgcacgg tattccggaa acttcagacg gctttcggag
     8221 tggatgaaat ccaccaggcc agcgcctttc ggacaaagtg caccgcggtt gaccgggtga
     8281 tccggatcgc cttcgatatg gaagatagat gctttggcgt tttttgctcc gtcaccgagg
     8341 ctgtacatca acagcccaca gcctacggaa caataggtgc aggtattacg ggtttcgcgg
     8401 gtgcgcagca gtttatactg ccgggtttcc gcgagtgcta cgctgggtgc aaaacccagt
     8461 gccgctgccg tggtgcctgc cataccgcca gcgcagatct taaagaactg ccttctgctg
     8521 acctgcatgg attgctcctt gtttcgacat tgtcacgtcc catttacatt cgcttgctgc
     8581 gtgtgcaggg agtgggagtt atttttcttt gcggaagtgg ccgcaaaggt ccagaattgg
     8641 ctcaatttcc ctccatccag gaagggtttg taacagaata ccataatgtt ggtgtgtgtg
     8701 ttcttatccg gttaatagaa agtgaaaaaa acacagcaaa aagaaatcga aaatgtgaca
     8761 aatgtcacag gtgttcgtca aattgagtta tggcggcgtg atgatttaca acacccacgg
     8821 ttagatgagg tggcggaaga agttcccgtt gcgctggtct acaacggcat ttcgcatgtg
     8881 gtgatgatgg cgtcacccaa agaccttgag tactttgcgc tcggtttttc gctttccgag
     8941 gggataatag aaagtccgcg cgatatcttc ggcatagatg tcgttccttc ctgtaatggt
     9001 cttgaagtac aaattgagct ttccagccgc cgctttatgg ggttgaagga gcgccgccgg
     9061 gcgctggcgg gacgtacggg ctgtggcgta tgcggagtgg agcaacttaa tgacatcgga
     9121 aaaccagtgc agccgctgcc gttcacccag acgtttgatc tcaacaaact ggatgatgca
     9181 ttacgtcatc tcaatgattt ccagccagtg gggcaactga ccggttgtac tcacgccgcc
     9241 gcctggatgt tgccatctgg cgaactggcg ggcgggcatg aagacgtggg tcgccatgtg
     9301 gcgctggaca aactgttagg ccgccgggcg caagaagggg aaagctggca gcaaggtgcg
     9361 gtgctggttt ccagtcgtgc cagttatgaa atggtgcaaa agtcggcgat gtgtggcgta
     9421 gagattttgt ttgcggtgtc tgccgcgacc acgcttgctg tagaagtggc tgagcgctgt
     9481 aatctgacgc tggtgggctt ttgtaaaccg gggcgggcaa cggtttatac gcatccgcag
     9541 cgtttgagca attaattaaa ataagtcctg cgatatatat taaattgcag gacttattca
     9601 tttcgtgaat tttattgttt tatttataaa taaaacgcgt gtagtggcgt tttatttttt
     9661 cattt
//
LOCUS       DAZUGK010000173         9659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZUGK010000173 DAZUGK010000000
VERSION     DAZUGK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.174"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHY5W_003697"
     CDS             complement(<1..458)
                     /locus_tag="ACHY5W_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HFS4448510.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTN"
     gene            561..1331
                     /gene="yqeC"
                     /locus_tag="ACHY5W_003698"
     CDS             561..1331
                     /gene="yqeC"
                     /locus_tag="ACHY5W_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFS4448511.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     NTENVHRWSQFADITGLTTDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1379..3004
                     /gene="yqeB"
                     /locus_tag="ACHY5W_003699"
     CDS             1379..3004
                     /gene="yqeB"
                     /locus_tag="ACHY5W_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFS4448512.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLVSRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3225..4157)
                     /gene="arcC"
                     /locus_tag="ACHY5W_003700"
     CDS             complement(3225..4157)
                     /gene="arcC"
                     /locus_tag="ACHY5W_003700"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4448513.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4205..5590)
                     /gene="hyuA"
                     /locus_tag="ACHY5W_003701"
     CDS             complement(4205..5590)
                     /gene="hyuA"
                     /locus_tag="ACHY5W_003701"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFS4448514.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLSYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLIAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5643..6854)
                     /gene="ygeY"
                     /locus_tag="ACHY5W_003702"
     CDS             complement(5643..6854)
                     /gene="ygeY"
                     /locus_tag="ACHY5W_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFS4448515.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6912..8108)
                     /gene="dpaL"
                     /locus_tag="ACHY5W_003703"
     CDS             complement(6912..8108)
                     /gene="dpaL"
                     /locus_tag="ACHY5W_003703"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFS4448516.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8166..9353)
                     /gene="ygeW"
                     /locus_tag="ACHY5W_003704"
     CDS             complement(8166..9353)
                     /gene="ygeW"
                     /locus_tag="ACHY5W_003704"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFS4448517.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2253 a         2363 c         2537 g         2506 t
ORIGIN      
        1 atattggtga cattgggtcg ctggattgcc tgcatcaggc atggttttga cactaaaatc
       61 ggatggccag ggatgccatt atggagtggc aggattgcgc catcatttcg taacgaccag
      121 atttttctga aaatatcgat ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt
      181 tctgtttgca ctgcgggtac tgcggccttg actgatgtta gtaaaccctg ggcataatct
      241 gggttgtgaa taatagtgat attgctctgg ttcgcataac gttcgtgcag ttcattacca
      301 cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt gatacttgta
      361 tcaagaattg ttccctgttg ccagggtaac atcattttcc attgccccat tcttgatgat
      421 aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt atttataggg
      481 gctaataacg cctgataatt gcgttacttc ctgatggatt aagttggggg taatttatcc
      541 cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta ttcattgatt
      601 taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg aaaaccagct
      661 tgcttttttg gctagcagaa ctgctccagg ctagcggcag gcgtgtgtta atcacgacga
      721 caacacatat gtttatgcca acgtctcact ggcccgtggt tttctgtcgt gatcccgcca
      781 tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc tggaaagcga
      841 accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg caacgaccag
      901 agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta aaagcgcctg
      961 atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg atgggaggac
     1021 atattttggg cgcgaaagtg aacacagaaa atgtccatcg ctggtcgcag tttgctgata
     1081 ttaccgggtt aacaactgat gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc
     1141 accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc
     1201 gtttttctca atgtgagaat gcgattgcgc aaagcgagct ccttcaaccg ctgcaacaac
     1261 acgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat
     1321 ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg aggttggtat
     1381 gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg cgatggcgca
     1441 aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag tgcgcgccga
     1501 tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga ttgaagagtc
     1561 gcttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg ctcgtaacgg
     1621 tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca gcgtccatgg
     1681 tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc aaccgggcga tagcccagag
     1741 tgcggcacta ttaggatttg atattgccgt tgctgatatt tatcgcgaaa gcctcaatcc
     1801 tgaactattc ccaccatcaa ccacgcttct ccatgctgag tcgtttggtg cggcagtgga
     1861 agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc aggatcgtga
     1921 agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg taagtcgtcg
     1981 caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtagctgaag aacatattgc
     2041 ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg agatcgccat
     2101 cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa
     2161 accttctcat ccttccggac accagctggt ggtgattcgc ggtgcgggag atatcgccag
     2221 tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa
     2281 accgacagtg attcgttgta ccgtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac
     2341 ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg gaagcgatga aacttaccga
     2401 acgcggattc atccctgtga tggtagatcc cgcctgttca ttgcttgatg aactgaaacc
     2461 gctttgcgtg gtggacgcta ttctggcgaa acagaatttg ggaacgcggg cagatatggc
     2521 accagtaaca atcgcgcttg ggccgggctt tactgctggg aaggattgtc atgcggtaat
     2581 tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg cgcaggagaa
     2641 taccggtgtt cctggcaata ttatggggca taccacccga cgggtgatcc gtgctcctgc
     2701 tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat
     2761 tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc gtggcttgtt
     2821 gaacgacggc ctggcagtgg ttggtggttt taaaattggt gatatcgatc ctcgtggtga
     2881 aaccgctgat ttcaccagcg tttctgataa agcccgggcg attggcggcg gcgtacttga
     2941 ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca aaagaagtgc tggaagtggc
     3001 ttaaatgaaa atggccggat ggtattaccc acccggcctg agactgatga caaacgccaa
     3061 attgcctgat gcgctacgct tatcaggcct acaagtgcat tgcaatctat tgaatttgcg
     3121 atgttttgta ggacggataa ggcgctcgcg ccgcatccgg catcaacaaa gcgcacttac
     3181 ttgacaatct ttggccggat ggtattaccc acccggcctt taaattactg acaaatcacc
     3241 gttcctgttt tgccttcaat cccttctttc gctttgctca atacggtaat cagtgcttca
     3301 cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag catagaacct
     3361 ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag gcgatcgagc
     3421 cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt cagaatgatc
     3481 agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt atcgataacc
     3541 gcgctggcac cacgcagatg gttaccttca cgaataacag ggataccacc accgccaacg
     3601 gtgatcacca cctgacctgc atctaccaga gctttaaccg tttctttttc aatgatatca
     3661 accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc tttcagagtg
     3721 taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc gatcggcttg
     3781 gtcgggttga ggaatgccgg atcgttagcg tcgacttcga cctgagtaac cagcgttgct
     3841 acaggtttat taatgccccg agaaagcagt tcttctctta aagcgttttg tagatcgtaa
     3901 ccaatataac cctggcttaa tgcaacacag acagacatcg gcagcattgg cgagtgcgct
     3961 tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc ccacctgcgg cccgttacca
     4021 tgagtgacga taacttcatg tccctgggca attaaatcaa caattgcctg agaagtaatt
     4081 tttaccgctt tcatttgccc ggccaggtca tcgcccagcg cattcccgcc cagggcgaga
     4141 acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag atccctggca
     4201 gtggttagag cacgggaggg acaaacggtt tacgtcgcag gaaacgacct cgcccggctt
     4261 tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg
     4321 cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg agatggcgat
     4381 gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg
     4441 ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcattgcg ctggttaatt
     4501 caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac agtaactgca
     4561 tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct ttagaaattt
     4621 gcaggcgttg agccatcgag aacgtgcagt gatcggtcgc taccacgtca atcgcaccat
     4681 cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt gggctaagaa
     4741 tgaacttcat gccatcttct gtatcgtagc tgcgttcgtc caacaggaga tattgtgggc
     4801 aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa tccagaccta
     4861 agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt gccaggttaa
     4921 tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggca tgatagcgcg
     4981 gtgcgattaa ccctgcggcg ataaattccg cccgcttgct ggcgatagca gcatcatttt
     5041 ccgggtgcac ggtggtcagc gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct
     5101 cgtcatcgtt gagtttgtat tgatagctta aatagagttt aaaactgctc agtccttcct
     5161 cgaccatcat cgggatttcg tcgaggattg cgtgattaat gtgctggatc acaccgtgaa
     5221 agctgtaatc gatgaccgct ttatgggcgg cataaccacg ataaacctcc agttgatggc
     5281 gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt gtaccgccac
     5341 acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg ccgacatcaa
     5401 tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca gtggcatcaa
     5461 tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca atcccgcttt
     5521 caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg tttttgatca
     5581 atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata agtgaagagt
     5641 aattattcgg ttgccagcca gcttaacggg attgcagcgt acatcgcggc acaggtcacc
     5701 aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt acccgggcca
     5761 aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt tgagaaggtc
     5821 cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac cagtgcttta
     5881 acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt tgggtaaacc
     5941 aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt agctttctgt
     6001 actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc ccaagtcaga
     6061 cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg ggatgtgaag
     6121 aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc cagacgttgg
     6181 gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt gtcaccgcgt
     6241 tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc catacgaccg
     6301 cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa ttccgggcga
     6361 atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc ttcctgcacc
     6421 gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat gattttaccg
     6481 gcataaacca tagatgccat gccgccttcc tggtcggaag taccgcgacc accgatcagc
     6541 tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt gccaatgccg
     6601 acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat ataaccgaga
     6661 acgttgccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat ctcttcttta
     6721 atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg gaatagcaac catgtcgcgc
     6781 aggaagcgag tcatatccgc ctggtaatct tttgcttttt caagaatcag tttgaatgga
     6841 atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag ggagcagttc
     6901 caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc gcggtagtgc
     6961 ttcacgtcgg tgtcgccttc ggtgctgata accagcacca cggcatcttt gttcagcgcc
     7021 agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgccgcgag aacgcccaaa
     7081 ccgacagcgc cggattcacc ggagataatg cgcggatcgt tgccgtacgg attacccagc
     7141 acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt agcgcagtta
     7201 cgtaggattt cccagcccag cgggttaggt tcgccacagg ccaggcccgc catgatggtg
     7261 gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata aatacagtca
     7321 gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctatagac gtcgaccaga
     7381 taacccagca caccaccggc cattgctccg acaccggctt gcagcagaac gtgcgtcggg
     7441 gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc gtagccttgc
     7501 atgatccagg ttgggatttt ggtgtatcct tcccatgccg tgtcctgtac cacttcccag
     7561 ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt catatcggtg
     7621 acgatgcact cggcacccag attcagaatg gcgtcaacgc gttcctgagc agaacctttc
     7681 ggcatgtaaa tcaccgcgtt ctgaccgagt tgctgagcgg cccatgccac accgcgcccg
     7741 tggttgccgt cggtggtggt cgcaaaggtc attttttcgc cgatggcatt tttcaggtgc
     7801 tcaaatgaca gcgtttcgat atcaagatga tatttttcgc acaataattg agcgatggcg
     7861 tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc gttttgattc gtctttgacg
     7921 agaattttct tcacaccaaa aaggtttgcg agatcgtcca gcgcacaaag cggtgttggg
     7981 cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct ttgcgaaaag
     8041 agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt caatgagaaa
     8101 acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct gatcggcccg
     8161 aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag tgcacctggt
     8221 ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca gctggcttct
     8281 ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcacc ttctttacag
     8341 gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc atcacgggtc
     8401 agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg tgccagacac
     8461 tgtttttcca gtgcttttaa gccttcgtgg tcgttcgcac gcagcaattc agtacgctct
     8521 tccatcactt tgtaaggtgc ccatgacttc ggataaacga tgtctgcgtc tttgaaggct
     8581 tcttccatgc tggtgacctg acggaagcta ccaccggagg ctttagcatt gtttttcgcc
     8641 acttcaacca catccgggat caggtcgtag ccttccggat gggccagggt gacatccata
     8701 ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt gccatagctt
     8761 ggggagtagg cccaggtcat ggcgattttt ttacctttca ggttttccag tgaaccaaag
     8821 tgttcacgta accatgccag gtcagccatt gactgagtcg ggtgatcaat atcgcattgc
     8881 aggttcacta aagccggacg ttgcggcagc acaccctgct tgtaaccgtc atcaagtgca
     8941 gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc gcgaatacca
     9001 atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc gccgtgagcg
     9061 atttgtgatt tgccttcatc gagatcttgc tgtgcaaggc cgagcaggtt aagcgcggaa
     9121 gcataagaga agcgggtacg ggtggagttg tcgcggaata cggaaatacc taatccacta
     9181 ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc
     9241 agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga
     9301 aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag
     9361 tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg
     9421 gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg
     9481 caaaagacta atttgacgga taatgtgtgg ggttgatcac aaattgatgc cagcttcggg
     9541 ttgtttgtaa aatacgaaaa cgtttggctt tttttattgc gttatttatt tgctgctaag
     9601 tatcagaaat aagataaagg ggcaggctat ccctcgtcag tttgataaac tcaccgccg
//
LOCUS       DAZUGK010000174         9483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZUGK010000174 DAZUGK010000000
VERSION     DAZUGK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.175"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            222..1853
                     /gene="oppA"
                     /locus_tag="ACHY5W_003705"
     CDS             222..1853
                     /gene="oppA"
                     /locus_tag="ACHY5W_003705"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFS4448518.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1939..2859
                     /gene="oppB"
                     /locus_tag="ACHY5W_003706"
     CDS             1939..2859
                     /gene="oppB"
                     /locus_tag="ACHY5W_003706"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFS4448519.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2874..3782
                     /gene="oppC"
                     /locus_tag="ACHY5W_003707"
     CDS             2874..3782
                     /gene="oppC"
                     /locus_tag="ACHY5W_003707"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFS4448520.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLITLFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3794..4807
                     /gene="oppD"
                     /locus_tag="ACHY5W_003708"
     CDS             3794..4807
                     /gene="oppD"
                     /locus_tag="ACHY5W_003708"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFS4448521.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4804..5808
                     /gene="oppF"
                     /locus_tag="ACHY5W_003709"
     CDS             4804..5808
                     /gene="oppF"
                     /locus_tag="ACHY5W_003709"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFS4448522.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5861..6190)
                     /locus_tag="ACHY5W_003710"
     CDS             complement(5861..6190)
                     /locus_tag="ACHY5W_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFS4448523.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6225..7685)
                     /gene="cls"
                     /locus_tag="ACHY5W_003711"
     CDS             complement(6225..7685)
                     /gene="cls"
                     /locus_tag="ACHY5W_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4448524.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVTERLFYFFSPLL"
     gene            7828..8001
                     /locus_tag="ACHY5W_003712"
     CDS             7828..8001
                     /locus_tag="ACHY5W_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFS4448525.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8056..9309)
                     /gene="kch"
                     /locus_tag="ACHY5W_003713"
     CDS             complement(8056..9309)
                     /gene="kch"
                     /locus_tag="ACHY5W_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFS4448526.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2459 a         2247 c         2368 g         2409 t
ORIGIN      
        1 aagagcatcg cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa
       61 ggtgatcgtc tgaatcacca gaataaataa agtcggtgat agcaatccgt aacgataaag
      121 taacctgaca gcagaaagtc tccgagcctg tgcagggtcc cattccggga ttacacatgc
      181 tggttaatac cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga
      241 gaagtttagt agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag
      301 ctgatgtacc cgcaggcgtc acactggcgg aaaaacaaac gctggtacgt aacaatggtt
      361 cagaagttca gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc
      421 gagacctgtt tgaaggctta ctggtcagcg atcttgacgg ccatccggca cctggcgtcg
      481 ctgaatcctg ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga
      541 aatggtctga tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg
      601 ttgatccgaa caccgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta
      661 ttgatgaaat tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg
      721 atcacacatt agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc
      781 acccatcaac ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc
      841 agcctggtaa tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac
      901 gaatcgttct tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg
      961 taacctattt gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa
     1021 tcgacatgac ttataacaat atgccgatcg aattgttcca gaagctgaaa aaagagatcc
     1081 cggacgaagt tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga
     1141 aaccgccatt caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata
     1201 tcattgttaa taaagtgaaa gcgcagggcg acatgcccgc ctatggttac actccaccgt
     1261 atactgatgg cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta
     1321 acgaagaagc gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca
     1381 tcaacctgct gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat
     1441 tgtggaagaa aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc
     1501 tcgacacccg tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca
     1561 acgaaccaac ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt
     1621 ataagagccc ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc
     1681 agcgcacagc tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc
     1741 ctgtttatta ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca
     1801 aagatccgct ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat
     1861 acgtggggca ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac
     1921 aggccagaag gtagggcaat gttaaaattt attctacgtc gctgtctgga agcgattccg
     1981 acgctattta ttcttattac tatttcgttc tttatgatgc gcctcgcgcc gggaagccct
     2041 tttaccggcg aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat
     2101 cttaatgatc caatcatgac acagtatttc agctacctga aacaactggc gcacggtgat
     2161 ttcggtccat cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt
     2221 cccgtttctg ccaaactggg agccgcagca tttttccttg cggtaatact gggtgttagt
     2281 gctggcgtta ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg
     2341 gcaatgaccg gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt
     2401 gcgatcattt tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg
     2461 atattgccga tggtggcgtt gtcactcgct tatatcgcca gtattgcgcg tattacccgt
     2521 ggctctatga ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta
     2581 cctatgcggc ggatcatttt acgccacgca ttaaaacctg ctctgttacc cgtgctctcc
     2641 tatatgggcc ctgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat
     2701 ggtttgccgg ggattgggca attgttcgtt aatggtgcat tgaaccgtga ctattcctta
     2761 gtgttaagcc tgaccatcct ggttggtgct ttaaccattt tgtttaatgc cattgtcgat
     2821 gtgctatatg cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt
     2881 taagtaagaa aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc
     2941 gcagcttgtg gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc
     3001 tgatagtgct ggtgctgatc acgttatttg taatcctggc accgatgctt tcgcagtttg
     3061 cctatgacga tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact
     3121 actttggtac tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta
     3181 tctcactcat ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttacg
     3241 gttcgctttc cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa
     3301 tcctcaactc tttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa
     3361 acatcctgct gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg
     3421 tgcgtgggca aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg
     3481 gtgtatcgac gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg
     3541 tggtctacgc atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc
     3601 tggggttggg tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga
     3661 actcgatgga agtctctcca tggttactgt tgttcccagc gggattcctc gtggtgacgc
     3721 tgttttgttt caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt
     3781 aaggagtgca gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc
     3841 tgacgcactg ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt
     3901 cacggcggtc aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg
     3961 tgagtctggt tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa
     4021 cggacgcatt ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg
     4081 tgaactcaat aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc
     4141 gttgaatccc tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa
     4201 catgagcaaa gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc
     4261 ggaagcgcgt aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg
     4321 agtcatgatt gcgatggcat tgctatgtcg gcctaagctg ctgattgcgg atgaacccac
     4381 tactgcgctg gacgtcaccg tacaggcgca gatcatgacg ctattaaatg aactgaagcg
     4441 ggaatttaat accgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg
     4501 cgacaaagtg ctggtaatgt acgccgggcg cacgatggaa tatggcaacg cgcgcgatgt
     4561 cttttatcaa cccgttcatc cttattctat cggtttgctc aacgcggtgc ctcgtctcga
     4621 tgcggaaggt gaaacaatgt tgactattcc gggtaatccg ccaaacctgc tgcgattacc
     4681 gaaaggttgc ccgttccagc cacgttgtcc gcatgcgatg gaaatttgta gtagcgcacc
     4741 gccgctggaa gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact
     4801 gttatgaatg ctgtaaccga aggaagaaaa gtcctcctcg aaatcgccga tctgaaagtg
     4861 cactttgaaa tcaaagatgg caaacagtgg ttctggcaac caccgaaaac tctcaaagcc
     4921 gtagatggtg taactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg
     4981 ggatgcggta agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt
     5041 catgttgcct ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt
     5101 cgcagtgata ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc
     5161 atcggcgaga ttatcgctga accgctgcgt acctatcatc cgaaaatgtc acgccaggaa
     5221 gttcgcgagc gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac
     5281 cgctatccgc atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt
     5341 attcttgaac cgaagctgat tatctgtgat gagccggtgt cggcgctgga cgtgtcaatt
     5401 caggcgcagg tggtcaacct gctccagcag ttacaacgtg agatgggatt gtcattaatt
     5461 tttatcgctc acgacctggc cgtggtaaaa cacatttccg atcgtgtgtt agtgatgtat
     5521 ctcggccatg cggtagaact ggggacctat gatgaggtct accacaatcc actacatcct
     5581 tacaccaggg cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa
     5641 accatccagt tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt
     5701 ttccgtaccc gttgcccgat tgccggtcca gagtgcgcca aaacacgtcc tgttctggag
     5761 gggagtttca gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg
     5821 ctgacagttg tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat
     5881 ggcaaagttt gtgatctttt tcgcggcata acagaatgcg ggcaaaaaca tcgttgattt
     5941 caccatcttc actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat
     6001 cgaaatcaac atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct
     6061 cttcaaactg aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt
     6121 cgagaaaaat atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat
     6181 ccatatccat agtcactacc tgtttaacct ctgttggcga cgttttacag caacggactg
     6241 aagaagtaaa acagtcgctc ggtgacacgt tgccataatg gacgttttag ccataaacgg
     6301 gcatcgagca gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca
     6361 aaacctttat cgtcgattgc cagagtaatc tcgaaattta gccacagact ccgcatatca
     6421 aggttaacgg tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt
     6481 aacccgcctt caaactgata aattttaacc ccagcagcca acagctccgt aaagaatgcg
     6541 cgactggccc agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc
     6601 accccgcgct gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag
     6661 ggcgttgtca tgatcaaata ttcacgcgcc gaataagccg cagtcaataa tgcctggtga
     6721 atgagatctt ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc
     6781 tgttcaaacg gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca
     6841 atctcccagt cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc
     6901 gccatcagat caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc
     6961 atattcatgc tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg
     7021 cgcaggtcca tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact
     7081 tcaataccgg cattacgcat tagctcgggc cacgggctgc ggaaaaaagc tacactcccg
     7141 gcggagtcga gcatcaatcg gcaatgaatg ccgcgtcgcg cagccgccat taatgattca
     7201 gccacctggt ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg
     7261 cgcgcgagct ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagt
     7321 tgtagctgat tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat
     7381 ggcgcagcga cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta
     7441 agccattttg cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa
     7501 tggagctcgc caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc
     7561 aacagccagg ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg
     7621 cctgcaatga gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt
     7681 gtcatagatt agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat
     7741 ctggcaaata aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc
     7801 attctgttat tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg
     7861 cgcatgcagc gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga
     7921 aatatgcgta tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg
     7981 tttgcgatct acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc
     8041 ttccgtcttt aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc
     8101 atcgttatcg ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt
     8161 attaatctct tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga
     8221 gagaatgata tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc
     8281 gagaactgtt ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc
     8341 tgcatcgtta tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa
     8401 tactgaactg tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc
     8461 aagttgcttg atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg
     8521 attcagttgc aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc
     8581 tttacgatgc attgtatgat tgtttccttt tacaagtttg ttgaatcccc cgcggataag
     8641 cggaccaaaa attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga
     8701 aatagtgaac aatcgtgctg attcagaaac agggacaata tcgccatagc cgacggttga
     8761 catggtttct atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc
     8821 ttcgcttaaa taaagcgcac cgtaggtgga gtaaaacagt aacgtcgtga aactaataaa
     8881 tgcaaaaatt gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa
     8941 aaacaccagc gtaaaaatgc aaaatccaat actaaatttt agccagggat aaaaatgcag
     9001 ggtgtaaatt agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg
     9061 gaacagtaag ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt
     9121 tgccagcaag gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt
     9181 gaccgacata ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc
     9241 gagaatgtcg tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca
     9301 gtgactcact gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt
     9361 ctgaacatac atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta
     9421 atcaaaggtt ttgagcgagc aactgtacct caggaaaggt catttttcct gaatatgctc
     9481 aca
//
LOCUS       DAZUGK010000175         9418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZUGK010000175 DAZUGK010000000
VERSION     DAZUGK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.176"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..1211
                     /gene="ulaG"
                     /locus_tag="ACHY5W_003714"
     CDS             147..1211
                     /gene="ulaG"
                     /locus_tag="ACHY5W_003714"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4448527.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1319..2074
                     /gene="ulaR"
                     /locus_tag="ACHY5W_003715"
     CDS             1319..2074
                     /gene="ulaR"
                     /locus_tag="ACHY5W_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFS4448528.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2071..2820)
                     /gene="yjfP"
                     /locus_tag="ACHY5W_003716"
     CDS             complement(2071..2820)
                     /gene="yjfP"
                     /locus_tag="ACHY5W_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4448529.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3002..3331
                     /gene="bsmA"
                     /locus_tag="ACHY5W_003717"
     CDS             3002..3331
                     /gene="bsmA"
                     /locus_tag="ACHY5W_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFS4448530.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3480..3755
                     /gene="yjfN"
                     /locus_tag="ACHY5W_003718"
     CDS             3480..3755
                     /gene="yjfN"
                     /locus_tag="ACHY5W_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFS4448531.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3872..5497)
                     /locus_tag="ACHY5W_003719"
     CDS             complement(3872..5497)
                     /locus_tag="ACHY5W_003719"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFS4448532.1"
                     /translation="MHWQTHTVFNQAIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREIAHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5581..6744)
                     /locus_tag="ACHY5W_003720"
     CDS             complement(5581..6744)
                     /locus_tag="ACHY5W_003720"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS4448533.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLDVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGKNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6747..7385)
                     /locus_tag="ACHY5W_003721"
     CDS             complement(6747..7385)
                     /locus_tag="ACHY5W_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFS4448534.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7395..7793)
                     /locus_tag="ACHY5W_003722"
     CDS             complement(7395..7793)
                     /locus_tag="ACHY5W_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFS4448535.1"
                     /translation="MHILNSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7811..8470)
                     /locus_tag="ACHY5W_003723"
     CDS             complement(7811..8470)
                     /locus_tag="ACHY5W_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFS4448536.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8521..9219)
                     /locus_tag="ACHY5W_003724"
     CDS             complement(8521..9219)
                     /locus_tag="ACHY5W_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFS4448537.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9238..>9418)
                     /locus_tag="ACHY5W_003725"
     CDS             complement(9238..>9418)
                     /locus_tag="ACHY5W_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFS4448538.1"
                     /translation="NQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLV
                     DNALDLAEITEEYSH"
BASE COUNT         2447 a         2490 c         2240 g         2241 t
ORIGIN      
        1 taaatctttc catactcaaa atcaacaaca acttccctgg caaaaagtca aaaaatggtc
       61 attgccttcc tgaaaaaata gcgtcatgat taatcaaaac caatcacata ttgattcgaa
      121 ttgaactatc atgaaggtaa atggcgatga gtaaagtgaa aagtatcacc cgtgaatcct
      181 ggatcctgag cactttcccg gagtggggta gctggttgaa tgaagaaatt gaacaagaac
      241 aggtcgctcc tggcacattt gcgatgtggt ggcttggctg caccgggatc tggttgaaat
      301 cggaaggtgg caccaacgtt tgcgttgatt tctggtgcgg cactggcaaa caaagtcacg
      361 gtaacccgtt aatgaaacag ggtcaccaga tgcagcgcat ggctggcgtg aaaaaactgc
      421 agccgaacct gcgtaccacc ccgtttgttc ttgatccgtt tgcgattcgc cagatcgacg
      481 cggtactggc gactcacgat cacaacgatc atatcgacgt caacgtcgct gctgctgtga
      541 tgcagaactg tgctgatgac gtaccgttta tcggaccgaa aacctgtgtg gatttgtgga
      601 ttggctgggg cgtaccgaaa gagcgttgca tcgtggtcaa accgggcgat gtagtaaaag
      661 tgaaagacat tgaaattcat gcgcttgatg ctttcgaccg tactgcactg atcaccctgc
      721 ctgccgatca aaaagcggct ggcgtactgc cagatggcat ggacgatcgc gcggtgaact
      781 acctgttcaa aacgcctggc ggctccctgt atcacagcgg cgactcccac tactctaact
      841 attatgcgaa gcacggtaac gaacatcaga tcgacgtggc tttaggttca tacggcgaaa
      901 acccgcgcgg tatcaccgac aaaatgacca gcgccgatat gctgcgtatg ggtgaagcgc
      961 tgaatgcgaa agtggttatc ccgttccacc acgatatctg gtcaaacttc caggccgatc
     1021 cgcaagagat ccgcgtgctg tgggagatga aaaaagatcg tctgaagtat ggcttcaagc
     1081 cgtttatctg gcaggtgggc ggcaaattta cctggccgct ggataaagac aacttcgagt
     1141 accactatcc gcgcggtttc gatgattgct tcactattga accggatctg ccgttcaagt
     1201 cattcctgta atctgtcgtt tataccggat agcgtggaga agttcgcgct atccggcgta
     1261 gtatttcctg gctatttcaa atatcatcta aaaaaatcaa attttatcgg aatagctcat
     1321 gactgaagca caaagacatc aaatcctcct ggaaatgctc gcacaattgg gctttgtgac
     1381 cgttgagaaa gtcgttgagc gtctgggaat ttcgcctgcc actgcgcgac gcgatatcaa
     1441 taaacttgac gaaagcggca aactgaaaaa agtgcgcaat ggcgcagaag ctattaccca
     1501 acagcgcccg cgctggacgc cgatgaatct gcatcaggcg cagaatcacg atgaaaaagt
     1561 acgtattgct aaagcggcct cgcagctggt taatccgggc gaaagcgtag tcatcaactg
     1621 cggctccacc gcgtttctgc ttgggcggga aatgtgtggc aagccagtgc aaatcatcac
     1681 taattatcta ccgctggcaa attacctgat cgatcaagaa catgacagcg tgatcattat
     1741 gggtggacag tacaacaaaa gtcagtccat cactttaagc ccgcagggca gcgaaaacag
     1801 tctctatgcc gggcactgga tgtttaccag cggaaaaggg ctgaccgcag aagggttgta
     1861 taaaaccgat atgctgacag caatggcaga gcagaagatg ctgagcgtgg tagggaaact
     1921 ggtggtactg gttgatagca gtaagattgg cgaacgcgcg ggaatgcttt ttagccgtgc
     1981 cgatcaaatc gatatgctta tcaccggcaa aaatgctaac ccggaaatcc tgcaacaact
     2041 ggaagcgcaa ggggtcagca ttctgcgtgt ttaaagatgc tggcggaaaa atgtcacggc
     2101 agcatctaac gcctcaggcg taatgcggtg acgcacgcct ggctgccatg aacaggttag
     2161 cagtttatcc cgtcccgtct cgcttaaggc ctgctgcaaa cgtagtgatt cgtcggcagg
     2221 cacaacgtca tcgtcgaggc catgccacag cagtagaggt ctgtcactaa gttgttccag
     2281 gtggtttgtc gcttcccact ctgccagcgg cgcgacaatg ttattgaatt cattctgctg
     2341 tgctgccgtt tcaggtatca gcggtggaaa cagtgaacgg gcgagcgatg taaaatagcc
     2401 cgatcccatc atgctggcgg tacatttcac cgtggggtgg cgagcggtaa ttcccagtgc
     2461 cgtcatcgcg cccatcgacg cgccaccgac tgccagacgg tcatcaagca gccagttttc
     2521 ttcggctatt gccgcacgta aagtagtgaa ttcctgcata ctttgtagca agatttgcca
     2581 gaattgattt aaccgccgcg ctgcgtcacc actaaaacgg ctaccgtgat cgggcgcatc
     2641 cggcatgatc acccgcaaac cagcctgcgc cagcgcaacg gcaaaatagc tatacaccag
     2701 actggatgaa gtaaagccgt gataaaaaat cacgcacggt aacggggtat ctttttgtcc
     2761 gacaggataa gcatgaagaa cgggaatatc tgccagctcg cgtgattcta tttcaatcat
     2821 ctgtctctcc tttttgtctg gtatgacatg aagaaatcct tagcacaagt ttctgctggc
     2881 ggtattagtt cataatgttg agatgtgggt tacgctttcg tcacgttttc attcgaaaat
     2941 cgcgtaccag gtaacaattc gggaacattc ccccaaagtt aaattaataa ggcactacac
     3001 tatggttagc aggaaacgta atagcgttat ttaccggttt gccagtttat tattggtgtt
     3061 gatgttaagt gcctgtagcg cactgcaagg tacgccacag ccagcaccac cagtaaccga
     3121 ccatccgcaa gagattcgcc gcgaccagac gcaagggtta cagcgaatag gtagcgtaag
     3181 cacgatggtt cggggttctc cggatgacgc attagcagaa atcaaagcga aagctgtcgc
     3241 tgcaaaagct gattattacg ttgtcgtcat ggtggacgaa accatcgtga caggacagtg
     3301 gtattcacaa gccattttgt accgtaaata aatcagacaa cttacatagt cagctttaca
     3361 ttgctttgcg tccctatgcg tttacctgtg cacaataaat taccggctgg aagcctgggc
     3421 gtttattacc gcgatggaat gccctgcaac atgtggggaa acgaaaaatg gagctgacga
     3481 tgaaacaatt acttgcctca ccctcgctgc aattagtgac ttatcctgcg agcgccacgg
     3541 cacagtctgc agaattcgcc agtgctgact gcgtgacggg cctgaatgaa ataggtcaga
     3601 tctcagttag caatatttca ggggatccgc aggacgtgga acgtatcgtg gcgttaaagg
     3661 ccgatgaaca aggtgcgtca tggtaccgca ttattaccat gtacgaagac cagcagcctg
     3721 ataactggcg cgtacaggcg atactgtatg catgagtgtc atctacagca aatgaacaat
     3781 gccggatgcg gcgcgagcgc cttgtccggc ctacaatttt cacatatttc attgagttaa
     3841 tcgaaactag cgtcgcatca gtcgtatacg cttacacaca cactcccccc gtcgcccgca
     3901 gcaataaatc attctggatc tgctctgaca accgtacgcc gccgcgcgta tctaacatca
     3961 cctgacacca cgcctgcgcc attggcggag aggcatattt caacatttgc gcaccgcagc
     4021 ccagcaggaa tagctgatga gcaatctctc gccccagttc ttcagctggc ttacgcagct
     4081 gctgctgtaa acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc acttccacaa
     4141 atgcttccga caataagtcg tatacgcccg cttgcttatt gagaacgcgc aacacatcca
     4201 ggcacataat attgccggaa ccttcccaaa tactgtttac cggcatctcc cggtaaagcc
     4261 gcggcagctc gctctcctcg caataaccaa tgccgcccag cacctccatc gcttcggcca
     4321 caaacggcat acctcgtttg cagatcacaa attttgccgc aggcgtaaat aaacgcgccc
     4381 acagggcttc tttggcatcg gcacgccggt cccacgctcg cgcaagacga aacagcaacg
     4441 ccgtttgccc ttcaagctga agtgccatgc gacttaagac atgacgcata aggggctgtt
     4501 ggatcaatgg attaccaaaa acatggcgtt gatgtgcatg ataaatcgcc agcgaaaatg
     4561 cacggcgcat catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc ccccccattt
     4621 tcaggatcag acgaattcct tccccttcct gccccaataa ccagccaatg gcatcctgaa
     4681 actccacttc gcaactggcg ttagagcgat tacccagctt atctttcagc cgctcgaggc
     4741 gaatcgcgtt gcgttgcccg tcaggcaaaa agcgcggcac aaaaaagcag gacagaccgc
     4801 ccgcggtctg cgccagcacc agatgcgcat cgctttgcgg caccgagaaa aaccatttat
     4861 gccccaccag ccgataagag ccatcttcca aacgctctgc acgggtggtg ttgctcatga
     4921 catcggaacc gccctgcttt tccgtcattc ccatgccaat caacaaaccg cgtttttgcc
     4981 cacctggcaa taagtgagaa tcgtagcgat cgctcaacag cggcgtggtc cagtcctgaa
     5041 acggcgcggg taacatctgt aacaacaatg gcgtggcggc aaaggtcatc gttattggac
     5101 ataacgaccc agcctcaacc tgcgcatgta acataaaacg cgccgcgcgc gccacaaatg
     5161 cgccggagcg agcgtcttct tcccaggcaa gattgtgcac ccgattggta catagtgcct
     5221 gcatcagcag gtgccaggcg gggtgaaaac gcacatcgtc cagacggcgt ccttgcgcat
     5281 cgtagcgcaa taattcaggc ggattcacat tcgccagccg ccccagttca agggattcag
     5341 ccgttcctaa ctgctgacca atactggcta gaaaatcgct atcccagcca gcaccttcac
     5401 gcgttaccgc ttcgcaaagc gcgccatcag acaggtataa gttgctgtta tttaatggta
     5461 tagcttgatt aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc atcaatggca
     5521 gtcaccatta gtatggtcac tgccacggat tcctgacaga aagagattgc taaaacattc
     5581 ttagccagca atgtaatgcg gaacaaaacg cgaagtgtct ttagtgatca gcgtgttatc
     5641 ttcgcggata cccataccgc acgcttcatc atcgacaatc cagctaccaa tcagcgtata
     5701 actatcgcca aatcgcggta gcggttgaaa cgcctggtag atcatcggtt catcggcgta
     5761 atcaccatca gcgtggtcaa caacgttatt cttgccgtca aaaatggtga cgttgccgcc
     5821 ttcgcgcgaa tagattggtt tacgcacata gctttcgcca gcggcaatct gcggtttctc
     5881 gccatcgaac caggacgcaa gaagattagg atgaccagga aagaagcgcc aaagcagcgg
     5941 cattaaccct ttgttgctca aaatactttt ccataacggt tccacccatt gctcacgacg
     6001 tttgcgcagc agcggaccgt tatcgtcacg catcatccac tccagcggat acagcttaaa
     6061 tgctcgctgg atgacgttat catcaagatc ggtcagtacg ccgccgacgc ccaaaccgag
     6121 atcttcaatg tagataaacc gagactcctg ccctgcctgc tgggcgcagt cctgcaagta
     6181 cagcacggta gtcctgtctt catcggtgtc ctgacagcag cagaaataaa acggctcccg
     6241 actgtaaagc tcactaaagc gcgaaatcag gcgttcctga atggcattgt actgatcggc
     6301 atcacgcgga ataatgccgc tgcgccgggc atcctctagc cacaaccact ggaaataagc
     6361 cgactcatac aacgaggtcg gcgtatcggc gttgtactcc agcagcttca ccggcgcatt
     6421 gccacaccag gcaaaatcca tccggccata cagcgaagga tcgcgtgcgc gccagctttc
     6481 agcgatcaca tcccagtaca acggcgggat cgccagttgc gtcaggatct cttcatcttt
     6541 taccgcgcga tccaccacat caaggcacat ctgatgcagc tccgcagtcg gtttttcgat
     6601 ctgctcttca atctggcgca gagtaaagcg gtaagcccga ctctcatccc aatagatttc
     6661 attgtcgatg atatgaaagt cgaaaccgtt gtcagcggcg atctggtcca gatcccgccg
     6721 cacaggaacg ttgtgtctca gcatgattag cctccccaat gcccacgggc gctggaagaa
     6781 cgaccatagc cgccgcgcga aacggttgac gcttttttgg tggtaaagcc gcctgaagag
     6841 taagactctt ttttgccaga accggagcgc caggagtaat caccggaagt gttgcgccag
     6901 acggggcggg aagcaaatga ggatccgccg ctgttataga cgaacggctc atcgcgatct
     6961 ttacgaataa cccggcttaa caaaaatcca gaaacgaccg ggatccaact ctgttcaaca
     7021 ttgtcgtagt agcaattttc gtacttagac tgacagttct gctgagtcat attcttcgga
     7081 acatcggcat aaaatgctgc tttggcgttg ttccagccac gcgcgcaaat gtcggcatta
     7141 ttaccgtcat caatacaatc ctgcacagtc gagtaaaacg ttccgtcacc gtcgttatcg
     7201 acatcgctac tttcactcca tcctttgact attaaaaaag cggcaccgcc cattagcgcc
     7261 aacgttaagt atttttgtgc gtaacgattg cgacgcggtt caaaaggatt attcggtctg
     7321 ccaatgcgac tgatcgctcc gtgaccaatt ttactgttat ttcttgattt gcgttttctg
     7381 gccatgatcg tccattacca tgtcatacaa gctgcgttaa aaataccacc agccagtgcg
     7441 gcggttccca taaacattcc tgctgcggta ttgtgattaa taattttttc gcttaatgcg
     7501 ggcatataaa gtctgacacc agcaaaaacg agtaactgaa tcactagtgc aatcccgccc
     7561 caggcgaaat agtctggaat actcaccgca ttgattgccg cactggataa ggggatcacg
     7621 taacccaaca atgtaccgct gaatgccagt gacgctgcgg tattattgtt tttgattaat
     7681 tgccattcat tgtgcggtgt aattttggag tagataaaca gaaaaataat caccatagcc
     7741 acgccaataa aaaaataggc actaaaggca agaagtgaat tcagtatatg cattgtaaac
     7801 gtccttgtaa ttaaccaata atatgaagtg atgtcagcgg gatatcgaca cccaacgcgc
     7861 gcgaaaataa ccactctggc tcgccgagat cgttaaaaga ttcctcacca tttagcagca
     7921 aatattcgta agtatcttcg gtcacttgcc gctggtagcc catggtaaaa ttatggactt
     7981 cccatttagc atgattttga ttttctactt tttccagcat gtagacgggt tcgatatttc
     8041 ccggttcttc gctgttaaaa aatcgctgcc agcgtttttc ctgccagttt aaggtcattg
     8101 cccccatcgt tttggcgttg atcgcctcgc gccagtgact ctctttactg ataccgtagc
     8161 tctcttcata gacaaagagc ttgatatcat caatgtcatc aatatcttcg ccgccggtgg
     8221 tattgatttg cagaaactca tcgcctgaag tgtagtagcg gaaaatctga ctaccgccgc
     8281 ccagatcgat gtggctgaca gcggctaccg taaattcctc acccggcagc gcaatcagca
     8341 attcatcttc cagcaaacga aacgctaacg tgtcgagcgt aaagccacta ttgagatgaa
     8401 gtcccagcgg accgcgagcg atagcaggct tattatcctt gccaaacaga cgctggaaaa
     8461 aaccagacat attttatcct taaaatagtt gccagccttg cagtgaggct ggcaaaaaaa
     8521 ttactcgccc tgttggcgtt gcagtcttgc taatacatcc tgggcgctac ttttattgct
     8581 accgccaatt ccggcttccg ccagcttttc gtcaaggtcg cgaccgtctg cgactttttc
     8641 taactgtgcg gcagcatcca ggcgagcctg acgttcggcc tgacgcgttt gcaggcgttt
     8701 taaggattct gctgctgtcg aaacgctgga agatgcgcca acggtagaag tcgttacagc
     8761 ctgttgtgca cgctgcatgg cttcagtggc tttaacgact tccatttgct gctcaaactg
     8821 agcgatacgc tgcgctgtcg ctgtaactgc tttttccacg ccatcacggg agacttccag
     8881 attcgacaac acttgctctt cagcggtaat gagattctca aggcgcgcga tttcttcagc
     8941 aacttcgtta atcaacgacg gattaacgtt cttgcttaac gcttccagcg cacgggcttc
     9001 cagactggct ttgcgctcac gtaaatcttt cagcttatcg tgacttaatt tcacccgcgc
     9061 cagcagatca acgcgagatt ttccggcttt atcgagttca gctttagcgt cacgaatatg
     9121 ctgttccagc atgcgcacac cctgggtttc ttcaatggat tcttccgcct gggagataaa
     9181 cgatttcccc agcgtaaata aactttttaa aattcccata aatacccgtc cttgtaatta
     9241 gtgtgaatat tcttctgtaa tttcagccag atccaatgcg ttatctacca gcgaggttat
     9301 ctcaagcagg atatcttcaa gagaagattt aagcgataac gccccgaaaa caatgtaata
     9361 ctcttcctgt tgcacgctgg agattcctac cgatgacaac ggcatcatct tctgattt
//
LOCUS       DAZUGK010000176         9357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZUGK010000176 DAZUGK010000000
VERSION     DAZUGK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.177"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            312..1609
                     /gene="kgtP"
                     /locus_tag="ACHY5W_003726"
                     /pseudo
     CDS             312..1609
                     /gene="kgtP"
                     /locus_tag="ACHY5W_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
     gene            complement(1606..1929)
                     /gene="yfiM"
                     /locus_tag="ACHY5W_003727"
     CDS             complement(1606..1929)
                     /gene="yfiM"
                     /locus_tag="ACHY5W_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFS4448539.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMRRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="ACHY5W_003728"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="ACHY5W_003728"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFS4448540.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="ACHY5W_003729"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="ACHY5W_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFS4448541.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKQATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="ACHY5W_003730"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="ACHY5W_003730"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFS4448542.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPVEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     NYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="ACHY5W_003731"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="ACHY5W_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFS4448543.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /gene="yfiF"
                     /locus_tag="ACHY5W_003732"
     CDS             7529..8566
                     /gene="yfiF"
                     /locus_tag="ACHY5W_003732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS4448544.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="ACHY5W_003733"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="ACHY5W_003733"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4448545.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
BASE COUNT         2247 a         2405 c         2443 g         2262 t
ORIGIN      
        1 agtccatcct gacggattgc ctttttgcat tggcgcagaa aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catacgccag attcagcagc gaatacggct tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aaatttatcc ttaagctcct caataaccat
      181 tttcctgcta actaaattca tggttaaggt tgcataatga tacgcaacaa atgtataaca
      241 ttttctttac caaaagaaat aaacaaaagc gaccgacaaa agcatcggat tacggcagga
      301 gacataatgg catggctgaa agtactgtaa cggcagacag caaactgaca agtagtgata
      361 ctcgtcgccg catttgggcg attgtggggg cctcttcagg taatctggtc gagtggttcg
      421 atttctatgt ctactcgttc tgttcactct actttgccca catcttcttc ccttccggga
      481 acacgacgac tcaactacta caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc
      541 caataggcgg ttggctattt ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc
      601 tgctatcggt gtgtatgatg tgtttcggct cactggtaat cgcctgtttg cctggttatg
      661 aaacaatcgg tacgtgggct ccggcattat tgcttctcgc gcgtttattt cagggattat
      721 ccgttggcgg agaatatggc accagcgcca cctatatgag tgaagttgcc gttgaagggc
      781 gcaaaggttt ttacgcatca tttcagtatg tgacgttgat cggcggacaa ctgctagccc
      841 tactggttgt cgtggtttta caacacacca tggaagacgc tgcactcaga gagtggggat
      901 ggcgtattcc tttcgcgtta ggagctgtgt tagctgtttt ggcgttgtgg ttacgtcgtc
      961 agttagatga aacttcgcaa caagaaacgc gcgctttaaa agaagctgga tctctgaaag
     1021 gattatggcg caatcgccgt gcattcatca tggttctcgg tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gttgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttaatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aaactctttt gacgccccca aactgacaga
     1741 gaacatcaaa ccaaacatgg cactgcgatc ccggcgcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat cccttttagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc ggattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag cttcatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggccagcgcc
     3541 accatgccac gattatttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cggcaaattc ggcatcgatg ttatcagggt cggagatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gcgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gcgatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatccctt gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcccg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccatg ccatttttac gcgatcgaaa
     4441 atagcgtttg ccgcttgctg gacttcattg gctgtacgca ggtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcactggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tttgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcagagaaac atacttgctg
     4921 cggggatgat gctttaccgc ttcaattaat gcttgcgcac tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctatggag cagaatgtcc
     5041 agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagcgg gttagagatt
     5101 gccacatgtc ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagtgtcgcc
     5161 tgcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt ttctaccgcc
     5281 gaaaacagtt cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccgga agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggccg tacaatattg
     6301 gccttcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatatgagg cgggaaagac caccattggc tgatagttcg ggttttgtac
     6541 cagatccagc aaatcttgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcaaacat
     6661 caacaaacaa aagcggcttt ttgcttccac tggcgtgatc gttgaacaga gacataattt
     6721 ctctggtaat agacagcgtt ggcaacgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggcgctt tcggcactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gaatactgcg aataccaaag
     7021 cggctgctta attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac tggtagatca tctttcagca atttgtcgag cgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccag aagttttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaat gcatgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagcgga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taatggcaca accgtgcagc agtgggtaag
     8101 ccaggcaggt gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggcgttgtgg tgcaggatgc
     8221 ggcactgctg gagtcgggtg cggctatccg taccgcagaa ggcggcgcag agcacgttca
     8281 gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg cgggttacac
     8341 cgtagtgaca acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgttgc
     8521 aacaggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca ttttcccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccatgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgcgatc cccacaacaa
     8821 aaacaccacg ccttcgcgat gctggttaat caggctgatc actttatcgg tgaaggtttc
     8881 ccagccgagg ctggcgtggg aatgcgcctg acctgcgcgt accgtcaaca cagtattgag
     8941 tagcagaacg ccctgacgcg cccagctttc aagataacca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcagcagt gacggaggag tggcgattcc
     9061 ggggcgaacg gaaaatgcca gcccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc tgtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggcgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaatag ggttgctgct tctcttcagc cagcacatca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacaga
//
LOCUS       DAZUGK010000177         9329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZUGK010000177 DAZUGK010000000
VERSION     DAZUGK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.178"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            11..85
                     /gene="tnaC"
                     /locus_tag="ACHY5W_003734"
     CDS             11..85
                     /gene="tnaC"
                     /locus_tag="ACHY5W_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFS4448546.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            306..1721
                     /gene="tnaA"
                     /locus_tag="ACHY5W_003735"
     CDS             306..1721
                     /gene="tnaA"
                     /locus_tag="ACHY5W_003735"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFS4448547.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1812..3059
                     /gene="tnaB"
                     /locus_tag="ACHY5W_003736"
     CDS             1812..3059
                     /gene="tnaB"
                     /locus_tag="ACHY5W_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFS4448548.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3191..4366
                     /gene="mdtL"
                     /locus_tag="ACHY5W_003737"
     CDS             3191..4366
                     /gene="mdtL"
                     /locus_tag="ACHY5W_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFS4448549.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4341..5300
                     /gene="yidZ"
                     /locus_tag="ACHY5W_003738"
     CDS             4341..5300
                     /gene="yidZ"
                     /locus_tag="ACHY5W_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFS4448550.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5457..6206
                     /gene="yieE"
                     /locus_tag="ACHY5W_003739"
     CDS             5457..6206
                     /gene="yieE"
                     /locus_tag="ACHY5W_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFS4448551.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6228..6794
                     /gene="yieF"
                     /locus_tag="ACHY5W_003740"
                     /gene_synonym="chrR"
     CDS             6228..6794
                     /gene="yieF"
                     /locus_tag="ACHY5W_003740"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFS4448552.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6847..8184)
                     /gene="adeP"
                     /locus_tag="ACHY5W_003741"
     CDS             complement(6847..8184)
                     /gene="adeP"
                     /locus_tag="ACHY5W_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFS4448553.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8351..9016
                     /gene="yieH"
                     /locus_tag="ACHY5W_003742"
     CDS             8351..9016
                     /gene="yieH"
                     /locus_tag="ACHY5W_003742"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFS4448554.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            complement(9153..>9329)
                     /locus_tag="ACHY5W_003743"
     CDS             complement(9153..>9329)
                     /locus_tag="ACHY5W_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448555.1"
                     /translation="LNVKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNILDW
                     FERYPDIVKNITKA"
BASE COUNT         2305 a         2328 c         2311 g         2385 t
ORIGIN      
        1 taagtgcatt atgaatatct tacatatatg tgtgacctca aaatggttca atattgacaa
       61 caagattgtc gatcaccgcc cttgatttgc ccttctgtag ccatcaccag agccaaaccg
      121 attagattca atgtgatcta tttgtttgct atatcttaaa tttgcctttt gtaaaggcca
      181 tctctcgttt atttacttgt tttagtaaat gatggtgctt gcatatatat ctggcaaatt
      241 aatcggtatt gcagatgtaa tattcacagg gatcactgta attaaaataa atgaaggatt
      301 atgtaatgga aaactttaaa catctccctg aaccgttccg cattcgtgtt attgagccag
      361 taaaacgtac cactcgcgct tatcgtgaag aggcaattat taaatccggt atgaacccgt
      421 tcctgctgga tagcgaagat gtgtttatcg atttactgac cgacagcggc accggggcgg
      481 tgacgcagag tatgcaggcc gcgatgatgc gcggcgacga agcttacagc ggcagccgca
      541 gctactatgc gttagccgag tcagtgaaaa atatctttgg ttatcaatac accattccga
      601 ctcaccaggg ccgtggtgca gaacaaatct atattccggt actgattaaa aaacgcgagc
      661 aggaaaaagg cctggatcgc agcaaaatgg tggcgttctc taactatttc tttgatacca
      721 cgcagggcca tagccagatt aacggctgta ccgtgcgtaa cgtctatatc aaagaagcct
      781 tcgatacggg cgtgcgttac gactttaaag gcaactttga ccttgaggga ttagaacgcg
      841 gtattgaaga agttggtccg aataacgtgc cgtatatcgt tgcaaccatc accagtaact
      901 ccgcaggcgg tcagccggtt tcactggcaa atttaaaagc gatgtacagc atcgcgaaga
      961 aatacgatat tccggtggta atggactctg cacgctttgc tgaaaacgcc tatttcatca
     1021 agcagcgtga agcagaatac aaagactgga ccatcgagca gatcacccgc gaaacctaca
     1081 aatatgccga tatgctggcg atgtccgcca agaaagatgc gatggtgccg atgggcggct
     1141 tgctgtgcat gaaagacgac agcttctttg atgtgtatac cgagtgcaga accctttgcg
     1201 tggtacagga aggcttcccg acatatggcg gcctggaagg cggcgcgatg gagcgtctgg
     1261 ccgtaggtct gtatgacggc atgaatctgg actggctggc ttatcgtatt gcgcaggtgc
     1321 agtatctggt cgatggtctg gaagagattg gcgttgtctg ccagcaggcg ggcggtcacg
     1381 cggcattcgt tgatgccggt aaactgttgc cgcatatccc ggcagatcag ttcccggcac
     1441 aggcgctggc ctgcgagctg tataaagtcg ccggtatccg tgcggtagaa attggctctt
     1501 tcctgttagg ccgcgatccg aaaaccggta aacaactgcc atgcccggca gaactactgc
     1561 gtttaaccat tccgcgcgca acatatactc aaacacatat ggacttcatt attgaagcct
     1621 ttaaacatgt gaaagagaac gcggcgaata ttaaagggtt aacctttacc tacgaaccga
     1681 aagtattgcg tcacttcacc gcaaaactga aagaagttta attaatacta cagagtggct
     1741 ataaggatgt tagccactct cttaccctac atcctcaata acaaaaatag ccttcctcta
     1801 aaggtggcat catgactgat caagctgaaa aaaagcactc tgcattttgg ggtgttatgg
     1861 ttatagcagg tacagtaatc ggtggaggta tgtttgcttt acctgttgat cttgccggtg
     1921 cctggttttt ctggggtgcc tttatcctta tcattgcctg gttttcaatg cttcattccg
     1981 ggttattgtt attagaagca aatttaaatt atcccgtcgg ctccagtttt aacaccatca
     2041 ccaaagattt aatcggtaac acctggaaca ttatcagcgg tattaccgtt gcctttgttc
     2101 tctatatcct cacttatgcc tatatctctg ctaatggtgc gatcattagt gaaacgatat
     2161 caatgaattt gggttatcac gctaatccac gtattgtcgg gatctgcaca gccattttcg
     2221 ttgccagcgt attgtggata agctcgttag ccgccagtcg tattacctca ttgttcctcg
     2281 ggctgaagat tatctccttt gtgatcgtgt ttggttcttt cttcttccag gtcgattact
     2341 ccattctgcg cgatgccacc agctccactg cgggaacgtc ttacttcccg tatatcttta
     2401 tggctttgcc ggtgtgtctg gcgtcatttg gtttccacgg caatattccc agcctgatta
     2461 tttgctatgg caaacgcaaa gataagctaa tcaaaagcgt ggtcttcggt tcgctgctgg
     2521 cgctggtgat ttatctcttc tggctctatt gcaccatggg gaatattccg cgagaaagct
     2581 ttaaggcgat tatttcctca ggcggcaacg ttgattcact ggtgaaatcg ttcctcggca
     2641 ccaaacagca cggcattatc gagttttgcc tgctggtgtt ctccaactta gctgtcgcca
     2701 gctcgttttt tggtgtcacg ctgggattgt tcgattatct ggcggatctg tttaagattg
     2761 ataactccca cgccgggcgt ttcaaaaccg tgctgttaac cttcctgcca cccgcgttgt
     2821 tgtatctgat cttcccgaac ggctttattt acgggatcgg cggtgccgga ctgtgcgcca
     2881 ctatctgggc ggtcattatc cccgcggtgc tggcaataaa agctcgcaag aagtttccca
     2941 atcagatgtt cacggtctgg ggtggcaatc ttattccggc gattgtcatt ctctttggta
     3001 taaccgtaat tttgtgctgg ttcggcaacg tctttaacgt gttacctaaa tttggctaaa
     3061 tccttcaaga agccagtcat tcgctggctt cttgcctctc aggaaatcac ttatgtccaa
     3121 atggcaactc gcctgatcct ccttcaccac gtatgctttg cgtcacctta ctatcaggac
     3181 gctttagccc atgtcccgct ttttgatttg tagttttgcc ctggttttac tttatcccgc
     3241 tgggattgat atgtacctcg ttggtttacc gcgcatcgcc gccgatctca atgccagcga
     3301 agcgcagtta catattgcgt tctccgttta tctggcaggt atggcagctg cgatgttatt
     3361 tgccggtaaa gtggccgatc gttcagggag aaaaccggtc gccatacccg gcgcggcgct
     3421 atttattatc gcctcggtgt tctgttcact ggctgaaacc agcacgttat ttcttgcagg
     3481 gcgctttcta caggggttgg gcgcgggctg ttgttacgtg gtggcattcg ccattttgcg
     3541 cgacacgctg gatgatcgac gtcgggcaaa agtgctgtcg ttactcaacg gcattacctg
     3601 catcattccg gtgttagcgc cggtgctcgg tcatctgatt atgcttaaat tcccgtggca
     3661 gagtctgttc tggacgatgg caataatggg catcgcggta ctgatgttgt ctttgtttat
     3721 tttaaaagaa acgcgccctg ccgcccccgc agcttcggac aaatcacgag aaaatagcga
     3781 gtcgctgctt aatcggtttt tcctcagccg tgttgttatc accaccctca gcgtttcggt
     3841 gatcctcacc ttcgtcaaca cgtcgccggt attgctgatg gaaatcatgg gttttgagcg
     3901 cggagaatac gccaccatta tggcgttgac cgctggcgtc agcatgaccg tttcattttc
     3961 cacgccattt gcgctgggaa tttttaagcc acgtacgttg atgatcacct cacaggtgtt
     4021 attcctggcg gcgggcatca ctcttaccgt ttcaccttcc catgcggttt ctctgtttgg
     4081 tatcacgctg atttgcgccg gtttctcggt aggttttggt gtggcgatga gtcaggcgtt
     4141 agggccgttt tcattacgcg cgggcgtagc cagctcgacc ttaggtattg cgcaggtttg
     4201 cggttcgtca ctgtggattt ggctggcagc ggtggttggt atcggcgcat ggaatatgct
     4261 gatcgggatt ctgattgcct gtagcatagt gagcctgttg ctgattatgt tcgtcgcgcc
     4321 tggacgcccg gttgccgctc atgaagaaat ccatcaccac gcttgatctc aatctgctgc
     4381 tctgtctgca actgctgatg caggagcgca gcgtaaccaa agcggcgaag cggatgaacg
     4441 tgaccccttc ggcggtgagt aagtcgctgg caaagttaag agcgtggttt gacgacccgc
     4501 tctttgttaa ctcaccgctg gggctgtcgc ccacaccgct aatggtcagc atggagcaaa
     4561 atctggcgga atggatgcaa atgagcaacc tgctgctgga taaaccgcac caccagacac
     4621 cgcgcggcct gaagtttgag ctggcggcag aatcaccgct aatgatgatc atgcttaatg
     4681 cgctgtcgaa acagatctac caacgttacc cgcaggcgac catcaaatta cgtaactggg
     4741 attacgattc cttagatgcc attactcgtg gtgaagtgga tatcggtttt tccggtcgcg
     4801 aaagccatcc tcgttcgcgg gagctgttaa gctcgctacc gttagccatt gattatgaag
     4861 tgctgtttag tgatgtgccc tgcgtctggt tacgcaaaga tcatccggca ctgcatgaaa
     4921 cgtggaatct ggacaccttt ttacgttatc cgcatatcag catttgctgg gaacagagcg
     4981 atacctgggc gctggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga
     5041 gcctgccgga attcgagcag tcactgttta tggcagcgca gcccgataat ctgctcctgg
     5101 cgaccgcgcc gcgctactgt cagtactaca atcaactcca ccaactgccg ttggttgctc
     5161 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agtccctttt accctgctgt
     5221 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggatacc attaaaaatc
     5281 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccgatttca caataaaatg
     5341 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
     5401 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
     5461 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
     5521 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
     5581 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
     5641 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
     5701 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
     5761 atatggaact gcagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
     5821 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
     5881 cgcagctcat cacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
     5941 atccgcgcga tctgcaacta ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
     6001 atgtagaagc gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgccca
     6061 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cggctggaaa agcctgcccg
     6121 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     6181 ccgtgaaggc tttttcgcca aattgataga caaccacagg agtcatcatg tctgaaaaat
     6241 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     6301 gtaccctgcc gaaaattgct ccggcgagca tggaagtcaa tgcgttacct tccattgccg
     6361 acattcccct gtatgacgct gacgtacagc aggaagacgg ttttccagca acggttgaag
     6421 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     6481 actcggtacc aggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     6541 cgctggctgg taaaccggta ttgattcaga ccagctcaat gggcgtgatt ggtggcgcgc
     6601 gctgtcagta tcacctgcgc cagattctgg tttttctcga tgcaatggtg atgaacaagc
     6661 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     6721 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggcgag tttattcagc
     6781 gagttaagat ctaaataaaa aacccgccag caatcatgca tggcgggttt tatcgcgcta
     6841 tcgattttag tgagcgtcga taaacacaat cttcaggata aacagcagcg caacgatgat
     6901 tacgcacggg ctaaggtcac gcagacgccc ggtaccgatt ttcatcacac agtaggagat
     6961 aaagcccagc gcaatacctt cggtaatcga gaagctgaac ggcatcatca cagcggtaat
     7021 aaacgccgga acagattcag taagatcctg ccagttcacg cgagcaagac ttgaggtcat
     7081 caacacgcca acgtaaatca gcgcgccagc tgcagcgtag cctggcacca tccccgccag
     7141 cggcgacagg aagataacca gcaggaacag cagaccgaca acgactgccg tcagaccagt
     7201 acgaccacca accgatacgc cggaagagga ctcaatataa gccgtaacgg aagaagtacc
     7261 gataaacgaa ccggtcacgg aagagatact gtcgacatac agcgcctgct tcatgcgcgg
     7321 gaatttacct ttctcatcag ccagacctgc tttatcggtc acgccaatca acgtaccgga
     7381 ggagtcaaac aggttaacca gcatgaaaga gaaaatcacc cctgccagcc cgaggttaaa
     7441 cgacccggct aaatctacat gacccacgac agtcattacg ctcggcggcg cagaaacgat
     7501 gccgttgtag tggacatcac ccagcatcca gcccagcagc gtcgtcacca cgatagaaac
     7561 caacaccgcc gcgtgaatgt tgcgtgaggc cagaatagca atgatgaaga agccgaggat
     7621 gcccagcagc acgctgtgag aagtcagatt accgatgctc accagcgttt ccgggttagc
     7681 gacaatcaca cctgcatttt tcagccccat catgccaata aacagaccga taccgctggt
     7741 gatgcccaca cgcagactca ccggaatgtt ggcaatcatc cagtagcgaa cgcggaaaat
     7801 cgtcagtaat agcagaccaa tcgcgcccca gaagattgcg cccattccga cttgccacgg
     7861 caagcccatc gcctgcacaa cgacaaaagc gaagaacgca ttcaggccca tagcgggtgc
     7921 caatgcaact ggcaggttag caaacagccc catcataata ctgccgaatg cagcaatcag
     7981 acaggtagtg acgaagacgg cgctggtatc catgccagca acgccaagaa tttgcgggtt
     8041 aacaaaaacg atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca cttcggtccg
     8101 tgccgtcgtg ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct ggccagaagc
     8161 ctgggttgtg tgttgttgac tcattatcta tttccgaaca aggagggaaa atccgtcgct
     8221 atcgtatacc aaaatgagac aatatggacg gctgaagata actttttttc attgcatttg
     8281 cttatacggc aacgattgcg tcatgcgaat tggcacgacg aaaacgttaa actaacgata
     8341 aaggaaagac atgtcccaga tagaagcggt atttttcgac tgcgacggta cgctggtcga
     8401 tagcgaggtc atttgctctc gcgcctatgt cacgatgttc agggaatttg gcattcacgt
     8461 tgacctggaa gagattttta cccgttttaa aggcgtaaaa ctctacgaaa tcatcgacat
     8521 catcagtaaa gaacagggcg tgacgatggt ccaggccgat gccgagcacg tttaccgcgc
     8581 agaagtagca cgtctgtttg ataccgaact tgaggacatc gcaggagcga acgaattgct
     8641 ggcgagcatt aacgtgccga tgtgcgtcgt ctctaacggt ccggtcagca aaatgcagca
     8701 ttcgctgggt aagctcaaca tgctgcacta cttcccggaa aaactgttca gcggctacga
     8761 tatccagcgt tggaagcccg atccggcatt aatgttccat gcggcaaaag cgatgaatgt
     8821 gaacgttgaa aactgcattc tggtcgatga ctccagcgct ggcgcgcaat cagggataga
     8881 tgccggaatg gaagtgtttt acttctgcgc tgacccgcac aataaaccga tcgttcaccc
     8941 gaaagtcacc acctttaccg agctggcgca gttgcctgcg ctctggaagg cacgcggctg
     9001 gaatataacg cgataatcgc ctccttactt cagcgcttac acatcagtca cgctggtttg
     9061 agataaagaa caccaggtgt ctgatgagta agcgtctatt ccctataaat aaattatgtc
     9121 gttaatcgtc cagtcgctca ccctctgtat ggttaagcct tggttatatt ttttacaata
     9181 tcgggatacc gttcgaacca atccagaata ttacgcatcg cgtcactgat gtttttatcg
     9241 tctttatatt gattctcaag tctattattt cgaacaatag taagcttaga acgtatttta
     9301 tcatcacaat cgacaatttt cacattcaa
//
LOCUS       DAZUGK010000178         9277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZUGK010000178 DAZUGK010000000
VERSION     DAZUGK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.179"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(139..1158)
                     /gene="rspB"
                     /locus_tag="ACHY5W_003744"
     CDS             complement(139..1158)
                     /gene="rspB"
                     /locus_tag="ACHY5W_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFS4448556.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQLITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIEPEKLITHTFEFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1170..2384)
                     /gene="rspA"
                     /locus_tag="ACHY5W_003745"
     CDS             complement(1170..2384)
                     /gene="rspA"
                     /locus_tag="ACHY5W_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFS4448557.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2590..2880)
                     /locus_tag="ACHY5W_003746"
     CDS             complement(2590..2880)
                     /locus_tag="ACHY5W_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFS4448558.1"
                     /translation="MCEIIGCFLPWLWLKRNASIWLLLPAGISLALFVWLLTLHPAAS
                     GRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIVAGWGRA"
     gene            3051..3392
                     /gene="ynfB"
                     /locus_tag="ACHY5W_003747"
     CDS             3051..3392
                     /gene="ynfB"
                     /locus_tag="ACHY5W_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFS4448559.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3427..3987
                     /gene="speG"
                     /locus_tag="ACHY5W_003748"
     CDS             3427..3987
                     /gene="speG"
                     /locus_tag="ACHY5W_003748"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFS4448560.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKIPGQTLLKPTAQ"
     gene            complement(3990..4700)
                     /gene="ynfC"
                     /locus_tag="ACHY5W_003749"
     CDS             complement(3990..4700)
                     /gene="ynfC"
                     /locus_tag="ACHY5W_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFS4448561.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4808..5113
                     /gene="ynfD"
                     /locus_tag="ACHY5W_003750"
     CDS             4808..5113
                     /gene="ynfD"
                     /locus_tag="ACHY5W_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFS4448562.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5312..7738
                     /gene="ynfE"
                     /locus_tag="ACHY5W_003751"
     CDS             5312..7738
                     /gene="ynfE"
                     /locus_tag="ACHY5W_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFS4448563.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7799..>9277
                     /locus_tag="ACHY5W_003752"
     CDS             7799..>9277
                     /locus_tag="ACHY5W_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFS4448564.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLARESGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDS"
BASE COUNT         2522 a         2330 c         2176 g         2249 t
ORIGIN      
        1 ttcaagcgca gtgcccagcc atcccgatac tgctgctttc accaaatcct tagtgcttct
       61 ttcgtgtttt tctattgtca taatggttat ctctaaaaaa gaggtaagat gcgtactact
      121 tactcgccgt tattggtatt attcagaaaa agtgagtaag actttgcagc aatgtttttg
      181 atcctgttca aataaactaa tggcatcagc aacatgctgg aattcaaacg tatgggtaat
      241 taatttttct ggttcaatta accctttact taaccagtcg ataacgaccg ggaatttatt
      301 tgcatttaag cgtgaagaga aaatagagag ttcttttccg gtaattagtt gctgaatcac
      361 ttcagaaggt tcactggaga agcccatcaa tacaatacgt gccgctggag aagccagcgt
      421 tacggcctct ttcaggatag aaggatgaca agccgcatcg ataattaatg tcggcttgat
      481 gcctttttca gcgaaaatct cgccaagcgg tgtctggctg ttattaatca cccagtcagc
      541 cccgctctct ttcgcttttt ccagtcgttc atcaatgcga tcggcaacaa tcacattttt
      601 aacgttatag acgcctttta atacctgaac gatcgtcagg ccgattggac cggcaccgta
      661 aaccagaacg gtatcatttt cagtcggttg accatgaccg gttacgttag ccgcaatggt
      721 aaaaggttcg atcattaccg catattgatc ggccactgct tcaggaattt tccacgcatt
      781 ttttgccgga accacggcat attcactgaa accaccgtca gcgtgcacac ctaatacagc
      841 cagtgtcgta caaacgttcg gcttacctat tgagcacgga tagcaatgcc cacagctgac
      901 caccggatcg acagcaacac gttcaccgac tctggcgctt tccacgccgt cacccactgc
      961 atcaatgacg ccaaagaatt catgaccaat gacgcgcgga tatttcgcaa aaggattatg
     1021 cccacgataa atatggctat ctgaaccaca aattccggca agtttcactt ttactcgtac
     1081 ttcacccgct gacggggtgg gtatttcacg ttcgacaatc gccagttgat tcggtttttc
     1141 aattaatatg cttttcatta tcttactcct taccagttcc acagcgtgcc atcttccaga
     1201 cgtgcgactg gtagataagc aggttcatag ggatatttcg ccgccagctt ttcatcgaat
     1261 tcgataccaa gacccggttt gtctcccgga tgcatatagc cgttatcgaa agtccagttg
     1321 tgcgggaaga cttcgagcat ttgttcggaa taacccatgt attcctggac accgaaattg
     1381 gggacccaca gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa atcggaagga
     1441 ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat cggcaatccg gcgcataccg
     1501 gtaattccgc ctgcatgggt cagcgtggtg cggatataat cgataagttg ctcttcaatc
     1561 agttgcttgc agtcccagat gctgttgaag acttcaccca ccgcgatggg tgtgacggta
     1621 tgttggcgaa tgagacggaa gcattcctgg ttttccgcag gcgtcgggtc ttccatccag
     1681 aacatgcgat aatcttcaat gctttttcca aagcgcgccg cttcaatagg cgttaagcga
     1741 tggtgcatgt catgcagcaa atgttcatta aaaccaaact tgttacgtac cgcgtcaaac
     1801 aatttcggca tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc cggccactgt
     1861 cctttggttg cgggttcata agccagacct ttacctttcg acatgccgta ggtggttttc
     1921 ataccaggga tgccgcactg cacgcggatg gctttgaatc ccagctcctg atgacgggca
     1981 taatcatcca gagcttcatc aatactgtga ccggtggtat ggcaataaac catcacccct
     2041 tcacgagacg cgccgccgag taactggtaa agcggcatgt tggcagcttt ggctttaata
     2101 tcccacagcg ccatatcaac cgctgaaatg gccgacatcg taaccggacc gcgacgccag
     2161 taagcacctt tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc gcgaccaata
     2221 agctgcggac aaaggtgatc ctgcaaataa gaggccacgg aaagctcacg tccattgagg
     2281 gtggcatccc caaggcccgt aataccgtcc tcagtggtga tttttaatgt gacgaaatta
     2341 cgccccggac aggtaacaaa aacttcagcc tttacgatct tcatgttcga ttccttgcat
     2401 cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact accatacaag tatgaagatc
     2461 gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat ggttgacaaa
     2521 agatgaaata gaataccttt tgtcagctga cgcttcctcg tatcttattg ataaaatgga
     2581 tttatgttcc tacgcgcgcc cccagcccgc aacaatgatc aacatgccgc aaagcgcaat
     2641 caacgcaccc gtccagtcat aaagtgtcag tttcacgcca tccacaacgc gcagccacat
     2701 caacgccgtg cagacataaa cgccaccata agccgcgtaa acacgcccac tcgccgcagg
     2761 atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc cccgccggaa gcaacagcca
     2821 gatactggcg tttcgtttta accacaacca gggcagaaag catccaataa tttcacacag
     2881 cgcagtagca aaaaatagta atgttgttta aatcatcttt gtcacttatt gacatcatgt
     2941 atagttatag ggcgacataa tatcatcaat ataaacaccc tcctggtacg ttttgcgctc
     3001 gcagtggatg gtgtagaatc acctttaatc attcatacag ggaatgaatt atgaaaatca
     3061 ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc ttgcgcactg gcattgagca
     3121 caactgttca tgccgaaact aacaaactgg tgattgagtc tggcgacagt gcacaaagcc
     3181 gccagcacgc cgctatggaa aaagagcaat ggaatgacac gcgcaatctg cgtcagaaag
     3241 tgaataaacg cactgaaaaa gagtgggata aagccgacgc cgcttttgat aaccgcgata
     3301 aatgtgagca aagcgccaac atcaatgcct actgggagcc aaatactttg cgctgcctgg
     3361 accgtcgaac tggccgcgtt attaccccct aacctgttat tgatttaagg aatgtaagga
     3421 cacgttatgc caagcgccca cagtgttaag ctacgcccgc tggagcgtga agatttacgc
     3481 tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga ggaaccctac
     3541 gaagcctttg ttgaactctc tgatctgtat gataagcata ttcacgatca gagcgaacgg
     3601 cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt ggaaattaac
     3661 catgttcatc gccgcgcaga atttcagata attatctccc cggagtatca ggggaaaggt
     3721 ctggcaaccc gtgccgccaa attagcaatg gactatggct tcaccgttct caatctctat
     3781 aagctgtatc tgatcgtcga taaagagaat gaaaaagcga ttcacattta ccgcaagctt
     3841 ggctttacgg ttgaaggtga attgatgcat gagttcttta ttaatggtca atatcgtaat
     3901 gccattcgca tgtgtatatt ccagcatcag tatctggcag agcacaaaat accgggtcag
     3961 actctcctga agccgaccgc acaatagcat taataataat cgattgtatt tttgatggtg
     4021 taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgattagctg acagtccacc
     4081 ggattggcgt gactgtcata ttcacagctc tgttttacat taccaacccg ttgattgttc
     4141 agtaaagtaa ccgctgtgta atctaatttt ttgatcggat ccgttgatgg cgtggcgctg
     4201 acagataatg ttttgtcgtt acttttcgtg gttttaccca gcggataccc ctgatcatca
     4261 tagcgatatt ccatctgcat ctgtttgctg ctggctttaa tcacgaagcc attatcatcg
     4321 gtttcccaac tcaccccggc ggaaggtaat tccgctagct ggcattttcc ctgtaaacgt
     4381 actctcttct ccagcgtctc ggcatcacgg taataattgg cgtccagtac cagagcgacc
     4441 acggtattat tttccagatc cagtaattcg agtgaatcaa aacagccttc ttccgacaaa
     4501 gtccccgaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg agtgaaatct
     4561 tttaccggac cacgcagcgg atcaaaatcg aattcattag agaaactggc catctcaggg
     4621 gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga ggaatatcgc gagtaagcat
     4681 ggtagtaatt tgtatttcac aacagtcacc agagagtaga gatgattctc aatcatagta
     4741 gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt cgcgtaagga
     4801 gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg cctcacccgc
     4861 ggtactcgcc gcgccgggat cctgtgagcg catacagagc gatatatcac agcgcattat
     4921 caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg accaggttga
     4981 tcagcctgat tcccaggtcg tcgggcattg tgctaatgat acgcataaaa ttctctacac
     5041 ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct acccaggatg gagcgcctgc
     5101 cgaaccgcag taacacatta tcgactgaac gccggatatg acacatccgg cgatttgaac
     5161 atacaacata atcccaccat attactcata cccttctatt gatgtggatt aataattctt
     5221 aacccaaaat gggtagactc cctctattgt tagcgcgcta aatattcaat atataaactt
     5281 ttatataacg ataaagaaca gggagtgagt tatgtccaaa aatgaacaaa tggtgggcat
     5341 cagccgcaga acactcgtta aatctaccgc gataggttct ctggcgctgg ctgcaggcgg
     5401 tttttcttta ccgttcaccc tgcgcagtgc agcggcagcg gtacaacagg ccagcgagaa
     5461 agtggtctgg ggtgcctgtt ccgtcaactg tggtagccgc tgtgcacttc gtctgcatgt
     5521 taaagataat gaagtgacct gggtggaaac tgacaatacc ggcagcgatg agtacggcaa
     5581 ccatcaggta cgcgcctgtt tgcgcggtcg ctccatccgc cggcgtatta atcatcccga
     5641 tcgcttgaat tacccaatga aacgcgtggg taaacgcggt gaaggcaaat tcgaacggat
     5701 tagctgggat gaagccctgg atactatcgc cagtagcctg aaaaaaaccg tcgaacaata
     5761 tggcaatgag gctgtatata ttcagtactc ttcggggatt gttggcggca atatgacccg
     5821 ctcttcgcca tcagcctcgg cggtcaaacg cctgatgaac tgctacggcg gttcactcaa
     5881 tcagtatggc tcctacagca ccgcgcaaat ttcctgtgcc atgccctaca cctacggcag
     5941 taatgatggc aacagcacca cggatattga aaacagcaag ctggtcgtga tgtttggtaa
     6001 caacccggca gaaacccgca tgagcggcgg tggcatcact tatcttcttg aaaaagcgcg
     6061 cgagaaatcg aacgccaaaa tgattgttat cgatccgcga tataccgata cggctgctgg
     6121 tcgtgaagat gaatggcttc ctattcgccc gggcaccgat gccgcgctgg ttgcggggat
     6181 tgcctgggta ttgattaacg aaaatctcgt tgatcaacct tttctcgata aatactgcgt
     6241 cggctatgac gaaaaaacct tacccgcaga tgcacccaaa aatggtcact ataaagccta
     6301 tattcttggt gaaggagacg ataaaacagc gaaaacgccg cagtgggctt cgcaaattac
     6361 cggtatcccg gtggaccgta tcatcaaact ggcgcgtgaa attggcacag caaaacccgc
     6421 ttatatctgc cagggctggg ggccgcaacg ccaggcaaac ggcgaactga ccgcacgcgc
     6481 tattgctatg ttgcctattc tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc
     6541 gcgtgaatcg acctatacca ttaccataga gcgactgccg gtgctggata atccggtcaa
     6601 aacgtcaatt tcctgcttca gctggacaga tgctatcgat catggcccac aaatgacggc
     6661 aatccgcgac ggtgtccggg gcaaagataa actggatgtg cccattaagt tcatctggaa
     6721 ctacgcggga aataccctgg ttaatcagca ttctgacatc aacaaaacgc atgaaattct
     6781 gcaagacgaa tcgaaatgcg aaatgattgt ggtcatcgaa aactttatga cctcatcggc
     6841 aaaatatgcc gacattctgc tgccagacct gatgaccgtt gagcaggaag atattattcc
     6901 taacgactac gccggtaaca tgggatatct cattttcctc cagcctgtca ccagcgaaaa
     6961 attcgaacgc aaaccgattt actggatcct gagtgaagtc gcgaaacgtc ttggacctga
     7021 cgtctatcaa aagttcacag aaggtcgcac gcaggaacaa tggttacaac atctgtacgc
     7081 caaaatgctt gccaaagatc cggcgttacc gtcttacgac gaactgaaaa aaatgggtat
     7141 ctataagcgt aaagatccca atggtcattt tgtcgcctac aaagcatttc gtgacgaccc
     7201 cgaggcaaat ccacttaaaa caccttccgg taagattgaa atttattcca gcaagctggc
     7261 ggaaattgcc cgtacctggg aactggaaaa agatgaagtg ataagcccac tgccagttta
     7321 tgcctctacc tttgaaggct gggactcacc tgaacgtaga accttcccac tgcaactgtt
     7381 tggtttccat tacaaatccc gtactcactc gacctacggc aatattgatc tcctgaaggc
     7441 tgcctgccgt caggaggtgt ggatcaaccc tatagatgcg cagaaacgtg ggattgccaa
     7501 cggcgatatg gtgcgggtgt ttaaccatcg tggcgaagtt cggctaccag ccaaagtgac
     7561 accacgtatt ctccctggag ttagcgctat gggccaggga gcctggcacg aggccaatat
     7621 gtctggcgat aaaatcgacc atggaggctg tgtgaatacg ctaaccactc tgcgcccttc
     7681 accactggcg aagggaaacc cgcagcatac gaatctggtc gagatcgaaa aaatataacc
     7741 cactacaacc ataaattctg gcatgacatt ttgtttgaaa agcaataagt gagtaatgat
     7801 gaaaatccat accacagagg cgctcatgaa ggctgaaatc agccgtagaa gtctgatgaa
     7861 aacctccgca cttggcagtc ttgcgctggc aagcagtgct ttcactctgc cattttccca
     7921 aatggtccgg gcggcagagg ctccggtaga agagaaagcg gtctggagtt cctgtaccgt
     7981 taactgcggg agccgctgtc tgttacgttt gcatgtgaaa gatgacaccg tgtactgggt
     8041 ggagtctgat acgacaggtg acgacgtcta cggtaatcat caggttcgag cgtgtttacg
     8101 cgggcgctct atccgccgac ggatgaatca tcctgatagg ttgaaatatc ccatgaagcg
     8161 cgtcggcaag cgcggtgaag gtaaatttga acggataagt tgggacgaag ccctggatac
     8221 catcagtgat aatcttcggc ggatcctaaa agattacggc aatgaggctg tacatgtcct
     8281 gtacggaaca ggtgtagatg gcggaaacat caccaactca aacgtcccgt accgtctgat
     8341 gaactcttgc ggtggttttc tcagtcgcta tggcagctac agtaccgcac agatcagtgc
     8401 cgcaatgagt tatatgttcg gtgccaatga tggcaacagc ccggatgata tcgccaatac
     8461 gaaactggtc gttatgttcg gaaataaccc ggcagaaacg cggatgagcg gcggtggtgt
     8521 cacttactac gtcgagcaag cccgcgaacg ttcaaacgca cgcatgatcg tcatcgatcc
     8581 acgttataac gacacagctg ccgggcgtga agatgaatgg ctgcccattc gccctggcac
     8641 cgatggcgca ctggcctgtg cgattgcctg ggtactgatt actgaaaaca tggtcgatca
     8701 gccatttctc gacaaatatt gtgttggtta cgatgaaaaa acgctgcccg ccaacgcacc
     8761 acgtaacgcg cattataaag cctatattct gggcgaaggg cctgacggca tagctaaaac
     8821 gccggaatgg gcagcaaaaa tcaccagcat tccggcagaa aaaattatcc agttggcacg
     8881 agagagcggt tcagcaaaac ctgcttatat ttgtcagggt tgggggccac aacgacactc
     8941 caacggcgaa caaacatccc gcgctattgc catgctttcc gttctcaccg gtaacgtcgg
     9001 cataaacggc ggcaactcag gcgtacgcga aggtagctgg gatctggggg tagaatggtt
     9061 cccgatgctc gagaatcctg ttaaaacgca gatttccgtc tttacatgga cagatgccat
     9121 cgaccatggt acggaaatga ccgcgacccg tgatggtgtt cgtggaaaag aaaaactgga
     9181 tgtccccatc aagtttttat ggtgctacgc cagtaacaca ttgatcaatc aacatggcga
     9241 catcaatcac acccatgagg tgcttcagga tgacagc
//
LOCUS       DAZUGK010000179         9172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZUGK010000179 DAZUGK010000000
VERSION     DAZUGK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.180"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..735)
                     /gene="mlaA"
                     /locus_tag="ACHY5W_003753"
     CDS             complement(<1..735)
                     /gene="mlaA"
                     /locus_tag="ACHY5W_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFS4448565.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDL"
     gene            1029..1961
                     /locus_tag="ACHY5W_003754"
     CDS             1029..1961
                     /locus_tag="ACHY5W_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4448566.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWNDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2037..2111
                     /locus_tag="ACHY5W_003755"
     tRNA            2037..2111
                     /locus_tag="ACHY5W_003755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2070..2072,aa:Arg,seq:cct)
     gene            complement(2206..3141)
                     /gene="dsdC"
                     /locus_tag="ACHY5W_003756"
     CDS             complement(2206..3141)
                     /gene="dsdC"
                     /locus_tag="ACHY5W_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFS4448567.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDSLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEEILPVCSPEYAQRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            3359..4696
                     /gene="dsdX"
                     /locus_tag="ACHY5W_003757"
     CDS             3359..4696
                     /gene="dsdX"
                     /locus_tag="ACHY5W_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFS4448568.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            4714..6042
                     /gene="dsdA"
                     /locus_tag="ACHY5W_003758"
     CDS             4714..6042
                     /gene="dsdA"
                     /locus_tag="ACHY5W_003758"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFS4448569.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFEVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGIHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMT
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(6150..7688)
                     /gene="emrY"
                     /locus_tag="ACHY5W_003759"
     CDS             complement(6150..7688)
                     /gene="emrY"
                     /locus_tag="ACHY5W_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFS4448570.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(7688..8851)
                     /gene="emrK"
                     /locus_tag="ACHY5W_003760"
     CDS             complement(7688..8851)
                     /gene="emrK"
                     /locus_tag="ACHY5W_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFS4448571.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATTALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2455 a         2149 c         2121 g         2447 t
ORIGIN      
        1 taaatcatcc tgaatcgctt gcgcgttcgg attttcctgc ggtttgagtt cgccgccatt
       61 agcgatgaaa tcatgacgct ggaagtacgc ttcgcgcacc ataatataag gatcggacga
      121 ctgacgcagc agaccgtcgg aatccaacaa ctgcgcacga gtttctatcc cttcaagcgt
      181 ccatttaccc acagacatcg gccaggtcag ccaggaaaga accgggtaaa gaccatccgc
      241 catatcacca ccgtcatcac gcagcgtgaa gctaccgtag aatggtaact gaacgtacgg
      301 cccataaccc acgccataat gaccaagcgt actaccgaag cggtgaggtt cagtccgttg
      361 cagtttcggg ttcgccatcc ctgcaacatc aataaagccg cccatcccca aaatggtgtt
      421 caggaaaaag cgggtaaagt ggaccatccc ctgataaggg tcgccctgca agaagtagtt
      481 caccatcacc gcgggttctt caaggttgcc ggtaaagttg ctcaaaccgt tacgcgccgg
      541 ttgcggaaca taatcacgcc aggcgacagc gaccggtcga acaatatacg ggtctagtac
      601 gttgaagttg aagttgtaca tggtgcggtt gaacccttct aacgggtcag aacgcccttg
      661 ctgatctgta ccggaactcg cacaccccac cagaagcgta gttcccagag caagcgccga
      721 caggcgaagc ttcataaatg tctccctgtt tttttatggc ttatgcagtt tgccatccat
      781 gacggaacga taccgtatcc gcctgtttag gtgtgggcga ttgtaacagc acgtcaactg
      841 atgtccagac gccctgattt gctgatttga tcatagcctg gtaatcgccg ccctgtaggc
      901 tacttgattc tatagaaaca gaaaaaggca aacgcagcct tttctacatt ttcagagtaa
      961 ctcccgccag ttgcgagcaa aaaagccgct acgctttagc tatacgtgct aattcaagag
     1021 aagagaccat ggacaacgac aaaattgatc aacacagcga cgaaattgaa gttgagagcg
     1081 aagaaaaaga gcgcggcaaa aaaatagaaa tagatgaaga ccgactcccc tcccgggcga
     1141 tggctattca tgagcatatc cgccaggatg gtgaaaaaga gctggaacgc gacgcaatgg
     1201 cactactgtg gtcagccatt gcggcgggtc tgtcgatggg cgcttcatta ctggcaaaag
     1261 ggatatttca tgtcgaactg gaaggtgtgc cgggcagctt cttgctggag aatctcggtt
     1321 atacctttgg ttttattatc gtcattatgg cccgccagca attatttacc gaaaacaccg
     1381 tgactgcggt actacccgtc atgcaaaaac cgacaatgag caacgttggc ttacttatgc
     1441 ggttatgggg cgtcgtgctg ctgggtaata ttctcgggac aggtattgcg gcgtgggcat
     1501 ttgaatatat gcctatcttc aatgaagaaa ctcgcgatgc atttgtcaaa atcggcatgg
     1561 atgtgatgaa gaacaccccc agcgagatgt ttgccaacgc gatcatttcc ggctggctga
     1621 tcgccactat ggtatggatg tttcctgcag cgggtgcggc aaagattgtg gtgattatat
     1681 tgatgacctg gcttattgcc ctgggtgaca ccactcacat cgttgtcggt tctgttgaaa
     1741 tcctctatct ggtgtttaac ggtacgctgc actggaacga tttcatctgg cccttcgcac
     1801 tacctacttt agcggggaac atctgcggcg gcacctttat cttcgcgtta atgagtcatg
     1861 cacagatccg taacgacatg agcaacaagc gcaaagcaga agcacgccaa aaagcagaac
     1921 gtgcggaaaa cattaagaaa aatgataaaa acccggcata aatggcgagg gtttaagcaa
     1981 tcgcacggca gcgtacttac cccgcagtcc attagcgggt atactcatgc cgcattgtcc
     2041 tcttagttaa atggatataa cgagcccctc ctaagggcta attgcaggtt cgattcctgc
     2101 aggggacacc atccttgtat tttccctaaa gccacataaa aaccgtaaat taaattcgaa
     2161 ctagcaggcc gaataaggca caccaggaac tcgtggtttt attcattatg aagtggtttt
     2221 tacctgttct cttagccagg taataaacgc ttcaattttt ggccactgcc tgcccggtaa
     2281 tgtggtgatg taataatgct gatggcattt caccgtcata tcgccaaacg gcgcgacgag
     2341 ctcaccactg gcaagccttt tttgtaccag acgtttcctt cccatcgcca ccccaatatg
     2401 attcatcgcg gcgataacgg ctaaatcaga gcgatcaaag ccaattccag aagatgtcgg
     2461 caaattaacc gcataatgtt gcgcccaact atgccattca tccgtaccgg agtcgttgct
     2521 ccatgcctgt ctgtcatgga gcaacgtaca gtgacgcagg ttaattaccg tgttggttaa
     2581 atcatgtctt tgagcgtact ccgggctaca aactggcaag atttcttcat ccatcagaaa
     2641 gtgatgagcc aattgcgctg acggcgcgtc atcaaagtag attgccaaat cgattccggc
     2701 acgttgcaaa ttgacattgt cattaccagt gagcacggtg agcgaaatag acggatatcg
     2761 gcgtgtaaag tcacctaatg cgggcaccaa ccagcattgg gcgatagagg gccgggaata
     2821 cagagttaac gttcccgata actcctgatt tttgatatcc agaatttcct gattcaggct
     2881 atccagcgac gattttagcg cccaataaac gcgtttcccc tcgtgcgtta attccacttt
     2941 gcgatgggaa cggacaaaca actgaatgcc caattcttct tccagctgat tgatacggtg
     3001 acttaccgca ctggggctca gcgacaattc ctctgccgcc agggcgaagg actgatgcct
     3061 ggcagccact tcaaaagtat gcatttttga tagttgccag ccgttaagca gtcgatttct
     3121 tatttcacga aggggttcca taatcacctc atttttctct taagtgtaaa aaaatagcgg
     3181 caaaatttca gctgtgagat gagctaaagt gaaccatatc tcaattcacc ttcattttta
     3241 gaggtaaatc actccattga tgcaatttac ctcatgtgaa aggcaaattt tatcgtttgt
     3301 cagcctgcgt tgtttttttg tccaatatca tcaggttaat cacaggggaa ggtgagatat
     3361 gcactctcaa atctgggttg tgagcacgct gcttatcagc atcgtgttaa ttgtactgac
     3421 catcgtgaag ttcaaattcc acccgtttct ggcgctgttg ctggccagct tcttcgtggg
     3481 aacgatgatg ggtatgggac cactggatat ggtaaatgct attgaaagtg gaattggcgg
     3541 aacgctgggg ttccttgctg cggttatcgg cctgggcacg atactgggaa aaatgatgga
     3601 agtatccggg gccgcagaaa gaattggtct gacacttcaa cgctgtcgct ggctttcagc
     3661 tgatgtcatt atggtgctgg ttggcctgat ttgcggcatc acgctatttg ttgaagtggg
     3721 cgtcgtgcta ttgattcctc tggctttttc aattgccaaa aaaaccaata cctcattgtt
     3781 aaagctggcc attccgctat gtaccgcatt gatggcagtg cactgcgtgg ttcccccaca
     3841 tccggctgct ttatatgttg ccaataagct gggcgcagat atcggttcgg tgatcgtcta
     3901 cggtttgctg gttgggctga tggcatcact gatcggtggc ccacttttcc ttaaatttct
     3961 ggggcaacga ctgcccttta aacctgtacc cacagagttt gccgatctca aagttcgcga
     4021 tgaaaaaaca ctaccgtcat taggcgcaac gttattcacc atactgctac ccattgcgct
     4081 gatgttggtt aaaacgattg ccgaattgaa tatggcgcgt gagagtggtt tgtatacctt
     4141 gcttgagttt attggcaacc ctatcactgc catgtttatc gccgtgtttg tcgcctatta
     4201 tgtgttgggt atacgccagc atatgagcat ggggacgatg ctcacacata cggaaaatgg
     4261 cttcggttct attgctaata ttttgctgat tatcggggcc ggaggcgcat tcaacgccat
     4321 tttaaaaagc agcagtctcg ctgatacgct ggcagttatt ctctccaata tgcatatgca
     4381 cccgattctt ctggcctggt tagtggctct tattctgcat gcggcagtgg gctccgctac
     4441 cgtggcaatg atgggggcaa cggcaattgt tgcacccatg ctgccgctgt atcccgacat
     4501 cagcccggaa attattgcga ttgctatcgg ttcaggcgcg attggctgca ctatcgttac
     4561 ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
     4621 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
     4681 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
     4741 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
     4801 cctggcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
     4861 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttcctgaa
     4921 actgctgcca ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa
     4981 cggctggaaa aggaatatca acaaccgatc agcgggcaac tgttactgaa aaaagatagc
     5041 catttgccca tttccggctc cataaaagca cgcggcggga tttatgaagt cctggcacac
     5101 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
     5161 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
     5221 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttgaggt gacagttcat
     5281 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
     5341 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
     5401 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
     5461 gtcgctggcc agcgtcttaa agcgcaattt gccgagcaag gtcgtattgt cgatgctgat
     5521 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
     5581 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
     5641 ccttgtatgc tgttaggcat ccatacaggg ttacacgatc agatttctgt tcaggatatt
     5701 ggtatcgaca accttaccgc agctgatggc cttgcagttg gtcgcgcatc aggctttgtc
     5761 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
     5821 gacatgcttg gctggctagc gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg
     5881 ggtatgaccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
     5941 agcgcagaac aactgcgtaa tgccactcat ctggtgtggg cgacgggagg cggaatggtg
     6001 ccggaagaag agatggagca atatctggca aaaggccgtt aataacgttt caacgcagca
     6061 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
     6121 ttacccgcta taaccccctg cttttaccat cacccaacgc ctttcgctgt aaacggcggt
     6181 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
     6241 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
     6301 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa tacagggtta
     6361 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg cccccacagc
     6421 gtcattgtca acgatgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
     6481 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
     6541 aagaaacagg caacggcgaa tccctgaaaa aactgcggca aaatgatacc tgtaaaatca
     6601 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaacta
     6661 aatgtcacta acaaccgcat gtctattttg ttgccataac gtcctatcaa aggtgaaatt
     6721 aatagtggca tgatgccgat gggcgcataa gcaagtccgg cccatatcgc attatacccc
     6781 atcgtttcct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
     6841 catgtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga
     6901 atcgggttct ctgaggtcga ctcccaaatg actaaagaga tcagagaaat aactgatact
     6961 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
     7021 ataatttgca agccaccaac accgagcact aacagggtca gtcctggtag attcattttg
     7081 accggtgaag tctcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
     7141 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc acaaatatag
     7201 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
     7261 gcaaatgtcc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     7321 aacggccccg ccattaaccc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     7381 gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt
     7441 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     7501 ccaaacgaag tgataaccca ggtaccttcg tctgttgatg ctcccagaaa gccagatatt
     7561 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     7621 aatgcaatag tgacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
     7681 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     7741 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca
     7801 gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac
     7861 caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac
     7921 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     7981 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     8041 gatcgctgat aatattgacc gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     8101 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggggc
     8161 tcactgtttc gccgacctga acacttctct gggcaatata gccggtaacc ggactcttaa
     8221 tatccgtacg tttaagcgcc aaccaggctt ctttagttgc atccgccgct tcaacgactt
     8281 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     8341 tagcggcatt caatgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
     8401 cttttgaaat aaccccctgc ttcgctaacg gcactcgacg gttataatct tctaaagatt
     8461 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtata
     8521 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcggtag
     8581 tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt
     8641 tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
     8701 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
     8761 cacctgaaaa cgcaataaat aaaactaccg ccaataaaga aaagtatttt cttctgttag
     8821 aatgtttttt atttgaatta atctgttcca cttttatctc tcatttcgca tagatgaaat
     8881 ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac
     8941 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
     9001 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag
     9061 tgctaatgtg aaactataaa tcatcggtac aatccctgat tttattgttg acatttcata
     9121 tatgccgact atttatatgg tatacttatc gaattatctt aacggaagct ca
//
LOCUS       DAZUGK010000180         9123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZUGK010000180 DAZUGK010000000
VERSION     DAZUGK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.181"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1314
                     /locus_tag="ACHY5W_003761"
                     /pseudo
     CDS             1..1314
                     /locus_tag="ACHY5W_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868837.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            1304..2518
                     /locus_tag="ACHY5W_003762"
     CDS             1304..2518
                     /locus_tag="ACHY5W_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4448572.1"
                     /translation="MARDSLYPVSVKAGKPILGMLPTGWQKLSLEKCLNIEARKAYIQ
                     DNQEYDLVTVKRSRGGVIRREHLKGKDISVKSQFYIKEGDFLISKRQIVHGACGLVPK
                     ELSGSIVSNEYCVLTGKSGFYLPYMEFLSESLYFQQTCFHSSIGVHIEKMIFKLDSWF
                     KWPFNIPPLSEQKRIVKILSTWDEAISVTEKLLTNSQQQKKALMQQLLTGQKRLLDEN
                     GVRFNGEWVYLTFDNAFIVANKKSAQVKSSDYLQSGSIPIIDQGQSRIAGYCNNLEVY
                     SDVPVIVFGDHTRCVKWIEFVFCPGADGTQVIKTSKILEPKLGYYLLSNTDIPNLGYS
                     RHMRELKEKDFRLPLDIKEQQKIAAVLSAADAEISTLEKKLACLKDEKKALMQQLLTG
                     KRRVKVDEAVAE"
     gene            2515..3198
                     /locus_tag="ACHY5W_003763"
     CDS             2515..3198
                     /locus_tag="ACHY5W_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFS4448573.1"
                     /translation="MKLTKSGPLIDREIDWLEEVLMKYGNDDSVLCFSELDGFLTAIV
                     SGPNTISPNTWLSAIWGRGDYHPRWTTEKEMTRFVGLCFQHMNDIAGCLYEAPEQFEP
                     IFNGREVKGKTYTIVEEWCFGYMKGRSLDDWSALPEALRPSLEAIALHGIEKNFPVVE
                     KMSPVQFEQSITLIQPAALALYQHWLSVRMSEASSRPVPVKGAEKLPGRNDPCQCGSG
                     KKFKKCCLH"
     gene            3143..5005
                     /locus_tag="ACHY5W_003764"
     CDS             3143..5005
                     /locus_tag="ACHY5W_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFS4448574.1"
                     /translation="MTRVSVAVVKNLKSAACTDKAEGGLLMAVNNLDRSRWYMGNVLW
                     FGGYNSKTDRENNFGFLLSENGNELFFHKNEISRNYTPADNAPVLFREGTGKNGKPTA
                     FNVHILDKTDEETAELLIEYLRAIIEEGVDFARWRYRDCVINFLTQSFGERAIIRLVT
                     SDIAATKVLPLFLKSRNYDNQFALFASDKNFDDLTAQQISPAVMPSSFIDNNIDQFAV
                     WVKRCSAATDCQGASTSDIINELLSHISISAILYLAFYDCISSERILEHRHDDIENFV
                     RRSFTKNKMDIQPFVRDAYQQKFSSREQFYKHSVISPFVNKYLIKQKMFRKDFSFVND
                     IESNTEISSDPEYFILSKLLPLIGRNDEQSVLSIILHEIWQGVLSGKIPVSHPSVFKL
                     FPQCSSLKIRSRNLKLSCEAFHWNAKQPDGTIEKKFLCRSKICHDPQVLPDLSRDYID
                     FTIYDWLAHYGMTYLIAGEPSKRDFPIKLAGYFNRIRELHSRLHCRSCGVLMVPDMKY
                     ARVEVSVWDTKSKGFVKKPFQAAYRLTVFKCASHSCEQFGIGHYINHCIGYKCSEIID
                     ARDLHEKCSEGRFICASCGSCCTTHQEKFGNVNKGETEQVKYNRLYRDSPFFSS"
     gene            5075..8359
                     /locus_tag="ACHY5W_003765"
     CDS             5075..8359
                     /locus_tag="ACHY5W_003765"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS4448575.1"
                     /translation="MDKHYQPKFQEEYSAKIPALTLLTSLGWTFLSPKQIMDYRGYKQ
                     DEVVLRPVLREELSKRSFMAGGKNCQLSEKALDNLISQVCSPALNEGLLKANERMYNH
                     LLYGIAVTEFVDGKKVNPTIALIDWEHPENNQFHFTEEFTVLRSGGVETRRPDIVCFV
                     NGIPLAVIEAKSPAGHGKKGPTIDEGISQSIRNQFNDEIPQLFVYSQLLLSINGHDGR
                     YGTCHTPMKFWAAWREEDITDPQMYALRNHQLSTEQIHALFDHRPSVDLKWYQQLIDG
                     GELAVSGQDKLLISLLSPERLLEMTRFFTLFDKKTGKIVARYQQVFGIKRLLERISTR
                     RPDGGREGGVIWHTTRSGKSYTMVFLSKALILHDSLKQCRIVVVTDRVDLEGQLSGTF
                     VSGGELAGKDDKAKAMATSGQKLAQQIGSGKERIIFTLIQKFNSATKLPECVNTSPDI
                     IVLIDEGHRSQGGENHVRMKLALPNAAFVAFTGTPLLKEDKTTNKFGPIVHAYTMQRA
                     VEDKAVTPLLYEERIPDLEVNDRAIDAWFDRITDGLSEAQKADLKRKYARKGEVYSAD
                     DRIRLIALDIATHFSKNIDEGLKGQLACDSKISAIKYKKYLDEAGLFESAVVISPPDT
                     REGNTEVDESKLPEVTKWWKDNVGMQDESVYTRNIISRFDTDDKLKLLIVVDKLLTGF
                     DEPKNTVLYIDKPLKSHNLIQAIARVNRLHPLKKFGLLIDYRGILAELDTTIGKYQDL
                     ASRTQGGYDIKDIDGLYSAMSSEYKRLPHLYNQLWAIFAGVKNKNDTEQLRAVLVPKM
                     EERDGEMVDIHQKTRDDFFEALTAFAGCLKVALQSATFFTDKSFTEQDRNLYKETVKQ
                     MSSLRQWAMQVSGEQVNYDDYAEQVKKLLDKHVTGVEVREPDGVYEVGKMGKSEKPEE
                     WDNNKTRNETDIIKTRVTKMIEQELRDDPYAQEAFSRLLRMAIEEAEKLFDHPLKQYL
                     LFREFEEQVKARKLSDIPDALAVNKHAQAYYGVFKKELPEVFVVKDVQVQDKWTKLAF
                     DVDNIIVKAVAENSLNPQDIEKVVKTSLLPLLFTACREIGAGMNQVNRIVETIIQILR
                     VGLMKS"
     gene            8356..9111
                     /locus_tag="ACHY5W_003766"
     CDS             8356..9111
                     /locus_tag="ACHY5W_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS4448576.1"
                     /translation="MSEKESPSMKALRIVYGDEVIVVQCVPRQVVKGRVLIKVYPDCR
                     VVASVPPETPEHEVLSALKKRGRWIYQQLRDFREQQTHIVPRQYVSGESHYYLGKQYQ
                     LKVTEDATVPQRVKMLRGRLEVTVRHKSAEKIKALLAEWYRERARDVFQRRLDLLIPQ
                     TLWVSERPPIRLRAMQTQWGNCSAKGCLTLNPWLVKASSECIDYVLLHELCHVAEHNH
                     SEEFYRLMGQVMPGWEKVKKRLDGMAGMLLADM"
BASE COUNT         2538 a         1945 c         2346 g         2294 t
ORIGIN      
        1 aacgagcgtt ttgtactgcc aaaaagtgcc agcttttatg cgctgtacga acgccgccat
       61 gagctgggta acggtgagcg tatcgatcag gctctgcatg caatcgaaga agccaacgga
      121 accaaactga aagatgccgg gaaaagcgtg ttccaggata tttcgtttaa caccgatcgt
      181 cttggcgaag aaaaacagaa aaacaccata ctgcgtcagc tactggaaga cttcgccggt
      241 gaagatctca acctgaagcc gagtcgtgtc ggtacgctgg acgttatcgg caatgcttac
      301 gaatacctga tcaaaaactt tgccgccagc ggtgggcaga aagccgggga gttctatacc
      361 ccgccggaag tttcggacct gattgcagaa ctgctcgatc cgcaaccggg tgataccatc
      421 tgcgatccag cctgtggttc cggttcatta ttgatgaagt gtgggcgtaa aattgtctcc
      481 gggcatgata gtcgtaacta tgccttgttc ggtcaggaag cgatcggttc tacgtggtcg
      541 ctggcgaaaa tgaatatgtt cctgcacggg gaagacaacc ataaaatcga gtggggcgat
      601 accattcgta acccgaagct gctcgataaa aatggcgacc tgatgctgtt tgatatcgtg
      661 accgctaacc caccgttcag cctcgataag tggggccatg acgaggccga gaacgataag
      721 tttggccgtt ttcgtcgcgg tgtaccgccg aagactaaag gagattacgc ttttatctcg
      781 catatgatcg aaaccctgaa accgggaact ggccgtatgg gcgtagttgt gccacacggt
      841 gtgcttttcc gtggctccag cgaaggtaaa attcgccaga aactgatcga cgaaaatttg
      901 ctggatgcgg taattggtct gccggaaaaa ctgttctacg gtacggggat tccggctgcg
      961 attctgattt tcaaaaagta gaaagttgat gacaaggtgc tgtttatcga tgccagccgt
     1021 gaattcaagg caggtaaaaa ccagaaccag cttagcgaag aaaacattaa gaaaatcgtc
     1081 aagacctacc gtgatggcga taacgtcgag aaatacgcct atcttgccag cctgaaggaa
     1141 attcaggaca acgattacaa cctcaatatt ccgcgctatg tcgatacctt tgaagaagag
     1201 gatgagattg acctggtggc ggtgcgagca gagcgtgagc aactgaaggt ggaactggcg
     1261 aagctggaag ttgagatggc agggtatttg aaggagttgg gttatggctc gtgattcgct
     1321 ttacccagtg agcgttaaag ctggaaaacc gatccttgga atgctaccta caggatggca
     1381 aaagttatca ttagaaaagt gcttaaatat agaggcaaga aaggcatata tacaagataa
     1441 tcaagagtat gacttagtta cagtaaagcg ctccagaggt ggcgtaataa gacgtgagca
     1501 tcttaaagga aaagatatct ccgtaaaaag tcaattctat attaaagaag gcgactttct
     1561 catatcaaaa cgacaaattg tgcacggtgc ttgcggtctt gtacctaaag aactttctgg
     1621 ctcaattgta tcgaatgaat attgtgtgct tacagggaag tccggttttt atcttcctta
     1681 tatggaattt ctatcggagt ctctatattt ccagcaaacc tgcttccact ctagcattgg
     1741 tgtgcacata gagaaaatga tttttaagtt agatagttgg tttaagtggc catttaatat
     1801 ccccccactg tctgaacaaa agagaatcgt taaaattctg tcaacctggg atgaggcgat
     1861 ttcggtgacg gaaaagcttc tcaccaacag ccagcaacag aaaaaagccc tgatgcagca
     1921 attgcttacc ggacaaaaac gcttgctgga tgagaatggg gtgaggttta atggggagtg
     1981 ggtatattta acgtttgata atgctttcat tgttgcaaat aaaaaatcgg cgcaagtcaa
     2041 atcttccgat tatttacagt caggttccat ccctataatc gatcaagggc aaagtcgaat
     2101 tgctgggtac tgtaataatt tagaggttta ttcggacgtg cctgtgattg tttttggcga
     2161 tcatacaaga tgcgtcaaat ggattgagtt tgtgttttgt cccggtgctg atggaacgca
     2221 agtaataaaa actagcaaaa tacttgaacc aaaacttgga tactatcttc tttcaaatac
     2281 agatattccg aatttaggtt acagcagaca tatgcgagaa cttaaagaga aagactttag
     2341 gttgccatta gatatcaaag aacaacaaaa aatcgccgcc gtcctttccg ctgccgacgc
     2401 tgaaatctcc acgctggaga aaaaactcgc ctgcctgaaa gacgagaaaa aagccctgat
     2461 gcaacagcta ctgactggca aacgtcgtgt taaggtcgat gaggcggttg ccgaatgaaa
     2521 ctgacgaaaa gcgggccgct gattgaccgg gaaatcgact ggctggaaga ggtgttgatg
     2581 aaatacggca atgatgactc tgtgctctgt ttctctgagc tggatggctt tctgacggcg
     2641 attgtctctg gcccgaatac gatttcccct aatacctggc tcagtgctat ctgggggcga
     2701 ggtgattacc accctcgctg gaccactgaa aaagagatga cgcgttttgt ggggctgtgt
     2761 tttcagcata tgaacgatat cgctggctgc ctgtatgagg cccctgaaca gtttgaaccg
     2821 attttcaatg gacgtgaggt gaaggggaag acatatacca tcgtcgaaga gtggtgtttc
     2881 gggtatatga aaggccggtc tctggatgac tggtcagcct tgccagaggc cttacgtccc
     2941 tcgctggagg cgatagccct tcatggtatt gaaaagaact tcccagtggt tgagaaaatg
     3001 tcacctgttc aatttgaaca gagtattact ctcattcagc ctgcggcact ggcgctttac
     3061 cagcactggt tgtcggtgag aatgtcagag gcatcatccc ggcctgttcc cgtgaagggg
     3121 gcagaaaaac tgcccggtcg taatgacccg tgtcagtgtg gcagtggtaa aaaatttaaa
     3181 aagtgctgcc tgcactgaca aagcggaagg gggcttgctc atggcagtga acaatctgga
     3241 tcgttcccgg tggtacatgg gaaatgttct ctggttcgga ggctacaaca gcaaaacgga
     3301 tcgggagaat aactttggct ttctcttatc agagaatggt aatgagctgt tttttcataa
     3361 gaatgagata agccggaact atacacccgc agacaatgca ccggtgctgt tcagggaagg
     3421 tacaggtaaa aatggtaagc ctacggcttt taatgttcac attcttgata aaacagatga
     3481 ggagacggct gagctcctta ttgaatatct ccgggctatt attgaggagg gcgttgattt
     3541 cgctcgttgg cgttatcgtg actgcgttat taattttctc actcagtcct ttggcgaaag
     3601 ggctataatt cggttagtta ccagtgatat agctgctaca aaagtcttgc cgctattttt
     3661 aaaatcacgt aactacgata accagtttgc acttttcgcc tctgataaga attttgatga
     3721 tttgaccgca caacaaatat ctcctgcagt catgccttcg tcatttatcg ataataacat
     3781 tgatcagttc gctgtttggg tgaaaaggtg ttctgcggct acggattgcc agggggcttc
     3841 aacatcggac atcatcaatg aattactcag ccatatatct ataagtgcta ttctctacct
     3901 ggcattttac gattgtataa gcagcgagcg gattcttgaa catcggcatg atgatataga
     3961 gaattttgtt cgacgtagtt tcacgaaaaa taaaatggat atacaaccat ttgtccgtga
     4021 tgcctatcag cagaaattct catcgagaga gcaattttat aaacattccg tgattagtcc
     4081 ttttgtcaat aagtatttga ttaaacagaa gatgttcagg aaagatttca gttttgttaa
     4141 tgatattgag tcgaatactg aaatttcttc tgaccctgaa tactttattc tgagtaaatt
     4201 gttaccgctg attgggcgta acgatgaaca gtccgttctg agtattattc tgcatgagat
     4261 atggcaggga gtgttgtcgg gtaaaatccc ggtaagccat ccttctgttt tcaaattgtt
     4321 cccacagtgt agctcattga aaattcgttc ccggaattta aaattatcct gtgaggcatt
     4381 tcactggaat gcaaaacaac ctgacggaac gatagagaaa aaattccttt gtcgttcgaa
     4441 aatctgccat gatccgcagg tactacccga cctcagtcgg gattacatcg acttcactat
     4501 ttatgactgg cttgcccact atggcatgac ctatctgatt gcaggcgaac cttctaagcg
     4561 ggatttccca attaaactgg ctgggtattt caatcgtatc agagaattac attcccgctt
     4621 gcattgccga agttgtgggg tgctgatggt tccagatatg aaatatgctc gtgtggaagt
     4681 cagcgtctgg gatacaaagt cgaaaggatt tgtgaaaaaa cctttccagg ccgcgtatcg
     4741 cctgaccgtc tttaaatgtg ccagtcattc gtgcgaacag tttggtatcg ggcattacat
     4801 taatcactgt attggttaca aatgcagcga aataattgac gccagggatt tgcatgaaaa
     4861 atgttcagaa ggacgattta tttgtgcatc ctgtggttcc tgctgtacga cgcatcagga
     4921 gaaatttggt aatgtaaaca agggcgaaac agaacaggtc aagtacaatc gcctttatcg
     4981 ggactcgccg ttcttttcat cataagtctt cgttcatttt gagtatgtcc gtaaggacat
     5041 acagtttcac ctcttagcca agaaggatta ccgggtggat aagcactacc aacctaaatt
     5101 ccaggaagaa tacagcgcca aaatcccggc actgacgtta ctgaccagcc tcggctggac
     5161 gtttttatct ccgaaacaga ttatggatta ccggggctat aaacaggatg aggtggtgct
     5221 gcgtccggtt ctgcgtgaag agctgtcgaa gcgttccttt atggcgggtg gcaaaaactg
     5281 ccagttatcg gaaaaagcac tggataacct gatatcacag gtctgttctc cggcgctgaa
     5341 tgaagggctg ctcaaagcca atgaacggat gtataaccat ctgctctacg gtatcgccgt
     5401 caccgaattt gtcgatggca agaaggtcaa ccccaccatt gcgctgatcg actgggaaca
     5461 tccggaaaat aaccagttcc attttactga agaatttacg gtattgcggt caggcggtgt
     5521 tgaaacccgc agaccggata ttgtctgctt tgttaatggt atcccgctgg ctgttattga
     5581 agcgaaaagc ccggctggtc atggcaagaa agggccaacc attgacgagg ggatatccca
     5641 gagtatccgc aaccagttca acgatgaaat cccgcagctg tttgtctaca gccagttatt
     5701 actttcgatt aacggtcatg acgggcgcta cggtacatgc catacgccaa tgaaattctg
     5761 ggcggcatgg cgcgaagaag atattaccga tccgcagatg tatgcgctgc gcaaccatca
     5821 gttatcgact gaacaaattc atgcgttgtt tgatcatcgt ccttcagtcg atctcaaatg
     5881 gtatcaacag ttaatcgatg ggggggaact ggcagtcagc gggcaggata agctgctcat
     5941 cagtctgctt tcaccggaac gtctgctgga gatgacccgc ttcttcaccc tgtttgataa
     6001 aaaaaccgga aaaattgttg cccgctatca gcaggtgttc gggataaaaa gactgctgga
     6061 acgcatcagt acccgcagac cagacggggg cagggaaggt ggggtgatct ggcataccac
     6121 gaggtcaggt aaatcttaca ccatggtatt cctcagcaag gcgctgatcc ttcatgacag
     6181 cctgaagcag tgtcgtattg tggtggtgac ggaccgcgtg gaccttgaag ggcagctcag
     6241 cggcactttt gtctctggtg gcgaactggc agggaaggat gataaggcca aagcgatggc
     6301 gacctcaggt cagaagctgg cacagcagat tggctctggt aaagaacgta taatctttac
     6361 ccttatccag aaatttaact cagcgacaaa attgcctgaa tgcgtcaata ccagccccga
     6421 catcatcgtt ctgattgatg aaggacaccg cagccagggg ggtgagaacc atgtgcgaat
     6481 gaaactggca ctgccgaacg ccgcctttgt ggcattcacc ggtacgccat tgctaaaaga
     6541 agataagacc accaataaat tcgggccgat tgtccacgct tataccatgc agcgagcggt
     6601 agaagataaa gccgttacac ctttgctgta tgaagaacgt attcccgacc ttgaggttaa
     6661 cgatcgggca atagatgcct ggtttgaccg tattaccgat gggttaagcg aagcgcaaaa
     6721 agccgacctt aaacgtaaat atgcccgtaa aggtgaggtc tacagtgcag atgaccgcat
     6781 ccggctgatc gcactcgata tcgccacaca cttctcgaag aatattgatg aggggctgaa
     6841 aggccaactt gcctgcgaca gcaagatttc cgccatcaaa tataaaaagt atcttgatga
     6901 agccgggttg tttgagtcag ctgtagttat cagcccgccg gatacccggg aagggaatac
     6961 tgaggtggat gaaagcaaac tgccggaagt gacgaaatgg tggaaagata acgtcggcat
     7021 gcaggatgag tctgtgtata cccgcaatat catcagtcgt ttcgatactg atgataagct
     7081 caaattactg atcgtcgttg ataaactgct caccggattt gatgaaccga agaataccgt
     7141 gctgtatatc gacaagccgc ttaagtccca caaccttatc caggcgattg caagggtaaa
     7201 ccgtctgcat ccactgaaaa agtttggcct gctgattgat tatcgcggca ttctggctga
     7261 actggacacg actatcggca aatatcagga tctcgcctcc cgaacccagg gaggatacga
     7321 catcaaggat attgacgggc tgtacagcgc catgagctct gaatataaac gtctgccgca
     7381 tctgtataac cagttgtggg ccatatttgc cggtgttaaa aacaaaaatg atactgagca
     7441 gttacgtgca gtactggtgc ctaaaatgga agaacgtgac ggagagatgg tcgatatcca
     7501 tcagaaaacc cgtgatgatt tcttcgaggc gttaacggcc tttgccggat gcctgaaagt
     7561 ggcactgcag tccgctacct tcttcaccga caagagcttt acggaacaag accgaaatct
     7621 ctataaagaa accgtaaaac agatgtccag ccttcgccag tgggcgatgc aggtaagtgg
     7681 agagcaggtc aattatgacg actacgctga gcaggtgaaa aaattgctgg ataagcatgt
     7741 taccggcgtt gaggttcgtg aaccggatgg cgtgtatgaa gtcggtaaaa tgggcaagag
     7801 cgaaaaaccc gaagagtggg ataacaataa aacccgcaat gaaaccgaca tcattaaaac
     7861 ccgtgtgacg aagatgattg agcaggagct gcgtgatgat ccgtatgctc aggaggcctt
     7921 ttccagactc ctgcgtatgg caatcgaaga ggctgaaaaa ctgttcgacc atccacttaa
     7981 gcagtacctc ttattccgtg agtttgaaga acaggttaaa gcccgtaagc tcagtgatat
     8041 cccggatgct ttggcggtaa acaagcatgc tcaggcatat tacggtgtat ttaaaaaaga
     8101 gctacctgag gtttttgtgg taaaagacgt tcaagtgcag gataagtgga cgaagctggc
     8161 ttttgacgtg gacaacatta tcgtcaaagc ggtggcagaa aactccctga acccccagga
     8221 cattgagaaa gtcgttaaga caagtctgct accactgctg tttacggcct gccgggaaat
     8281 tggtgccgga atgaatcagg ttaaccgtat tgtggaaacc atcatccaga tactgcgcgt
     8341 tggtttgatg aaatcatgag tgaaaaagaa tcgccatcaa tgaaagcgtt acgcattgtc
     8401 tatggtgatg aggtcatcgt cgtacagtgt gttccgcgtc aggtcgtaaa gggcagggtg
     8461 ctcatcaagg tttatccgga ctgccgggtt gtggcctctg taccgcctga aacaccggag
     8521 cacgaggtat tgtctgcgct gaaaaaacgt gggcgctgga tataccagca actgcgtgat
     8581 ttcagggagc aacagacaca cattgttccg cgtcagtatg tcagcggaga aagccattat
     8641 tatctcggta agcaatatca gttgaaagtg acagaagacg ctactgtgcc gcaacgagtg
     8701 aaaatgttgc gtgggcgtct ggaagtgact gtcagacaca aatcggcaga aaagattaaa
     8761 gccttgctgg ctgaatggta tcgggaacgt gccagagatg tttttcagcg caggcttgat
     8821 ttactgattc cgcagaccct ttgggtcagt gagcgtcctc cgatacgtct gcgggcgatg
     8881 caaacgcaat ggggcaactg ttcagcaaaa ggctgtctga ccctgaatcc atggctggtt
     8941 aaggcatcct cagaatgtat cgattatgtt ttgctacatg agttatgcca tgttgctgag
     9001 cacaaccaca gtgaagagtt ttaccgcctg atggggcagg ttatgcctgg gtgggaaaag
     9061 gtcaagaaac ggctggatgg catggctggg atgttgttgg ctgatatgta gtaagagccc
     9121 agc
//
LOCUS       DAZUGK010000181         9118 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZUGK010000181 DAZUGK010000000
VERSION     DAZUGK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.182"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..561)
                     /gene="pabA"
                     /locus_tag="ACHY5W_003767"
     CDS             complement(<1..561)
                     /gene="pabA"
                     /locus_tag="ACHY5W_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFS4448577.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(593..1195)
                     /locus_tag="ACHY5W_003768"
     CDS             complement(593..1195)
                     /locus_tag="ACHY5W_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFS4448578.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1185..1352)
                     /locus_tag="ACHY5W_003769"
     CDS             complement(1185..1352)
                     /locus_tag="ACHY5W_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFS4448579.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(1457..2029)
                     /gene="ppiA"
                     /locus_tag="ACHY5W_003770"
                     /gene_synonym="rotA"
     CDS             complement(1457..2029)
                     /gene="ppiA"
                     /locus_tag="ACHY5W_003770"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFS4448580.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2300..3481
                     /gene="tsgA"
                     /locus_tag="ACHY5W_003771"
     CDS             2300..3481
                     /gene="tsgA"
                     /locus_tag="ACHY5W_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFS4448581.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSHHRQHNTLSS
                     H"
     gene            3743..6286
                     /gene="nirB"
                     /locus_tag="ACHY5W_003772"
     CDS             3743..6286
                     /gene="nirB"
                     /locus_tag="ACHY5W_003772"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFS4448582.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLDNLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            6283..6609
                     /gene="nirD"
                     /locus_tag="ACHY5W_003773"
     CDS             6283..6609
                     /gene="nirD"
                     /locus_tag="ACHY5W_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFS4448583.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            6735..7541
                     /gene="nirC"
                     /locus_tag="ACHY5W_003774"
     CDS             6735..7541
                     /gene="nirC"
                     /locus_tag="ACHY5W_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFS4448584.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            7560..8933
                     /gene="cysG"
                     /locus_tag="ACHY5W_003775"
     CDS             7560..8933
                     /gene="cysG"
                     /locus_tag="ACHY5W_003775"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFS4448585.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTTVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         2122 a         2359 c         2390 g         2247 t
ORIGIN      
        1 gcgatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
       61 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
      121 gctccaggcc gtcacgtcaa agcaagctgg taatgagcca ggttccacca ccagcgaatg
      181 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
      241 tatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttac cgccaaatgc
      301 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
      361 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccgg gtgagatgac
      421 aactttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
      481 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
      541 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
      601 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
      661 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
      721 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
      781 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
      841 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
      901 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
      961 cacgcagttg ccctgcccag tcgaaaatat cctgatacag ctggcgatgg atagtgcgca
     1021 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
     1081 tttcgtaagc ggcctgttcc agccgttgtt gctggcggat gttcagccgg ttacgcatga
     1141 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtga
     1201 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
     1261 tcgacgcctt caagacggcg actggcctga aaattacgat tacgctgaag ctcccagaga
     1321 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
     1381 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
     1441 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
     1501 gcacattctg gtacggacca acgtcatgag tcggcacctg ggaaatctta tcggcaacgt
     1561 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
     1621 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
     1681 tacgcgccat cgcgatggtg ccacgcgtgt tgcgtaggcc gttatcggct tcatttttga
     1741 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
     1801 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
     1861 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
     1921 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
     1981 cagaaagagc gaaaacagca gccatcgccg ccagggtcga tttgaacatg agatttcctt
     2041 aacaaacaga gcagaaaaaa agcaagtgaa gagattctaa atagccttca agaacaaggc
     2101 catcccttta cctaatttta cgtatctgaa atatatgtaa cctgacgatc actttttatt
     2161 ccgtgatcaa aatcacctct taaaatgcaa tttagcaacc gattgcaata aaacatttaa
     2221 acagatcaca aaatcaccta aaatcgcccg ctcgcggcat atcccgctat gccattttat
     2281 ttttgaacgc aggccaatca tgactaacag caatcgcatc aagctcacat ggattagctt
     2341 tctctcctac gcactgactg gtgcgttggt tattgtcacc gggatggtga tgggaaatat
     2401 cgccgattat ttcaatctgc ctgtttccag tatgagtaac accttcacct tcctcaacgc
     2461 cggcatttta atctctatct tcctcaacgc ctggctgatg gaaatcgtcc cgttgaaaac
     2521 gcagttacgt tttggctttc tcctgatggt gctggcggtt gccggtttga tgttcagcca
     2581 cagcctggcg ctgttctcgg cggcgatgtt cattctcggg gtggtcagcg gcattactat
     2641 gtcgattggt acattcctga taacacaaat gtatgaaggg cgtcagcgcg gttcccgcct
     2701 gttatttacc gactccttct tcagtatggc tgggatgatt ttcccaatga tcgccgcgtt
     2761 tctactggcg cgcagcattg agtggtactg ggtttatgcc tgcatcgggc tggtgtatgt
     2821 cgctattttt attctgacct tcggctgtga gttcccggca ctgggcaaac atgcaccaaa
     2881 aacggatgct ccggtagcga aagaaaagtg ggggatcggc gtactgtttc tctctgttgc
     2941 ggcgctgtgc tacatcctcg gtcagttagg ttttatctcc tgggtgcctg agtatgccaa
     3001 aggcctgggc atgagcctga acgacgcggg cacgctggtg agtaacttct ggatgtcata
     3061 catggtcggc atgtgggcgt tcagctttat tcttcgcttc tttgatttgc agcgcattct
     3121 gaccgtactg gctggtctgg ctgcgattct gatgtatgtc tttaacaccg gaacaccggc
     3181 gcatatggcg tggtctattc ttgctctggg cttcttctcc agcgcaatct ataccaccat
     3241 catcaccctg ggttcccagc agaccaaagt accgtcgcca aaactggtta acttcgtcct
     3301 gacctgcggg accatcggta ctatgttgac ctttgtggtt accggcccga ttgttgaaca
     3361 tagcggtccg caggcggcac tgcttacagc aaacggtctg tacgctgtcg tctttgtgat
     3421 gtgcttcctg ttaggtttcg tcagccatca tcgtcagcat aacaccctgt cctctcatta
     3481 attgctcatg ccggacggca ctatcgtcgt ccggcctttt cctctcttcc cccgctacgt
     3541 gcatctattt ctataaaccc gttcattttg tctatttttt gcacaaacat gaaatgtcag
     3601 acaattccgt gacttaagaa aatttataca aatcagcaat atacccctta aggagtatat
     3661 aaaggtgaat ttgatttaca tcaataagcg gggttgctga atcgttaagg taggcggtaa
     3721 tagaaaagaa atcgaggcaa aaatgagcaa agtcagactc gcaattatcg gtaacggtat
     3781 ggtcggccat cgctttatcg aagatcttct tgataaatct gatgcggcca actttgatat
     3841 taccgttttc tgtgaagaac cgcgcatcgc ttatgaccgc gtacacctct cgtcttactt
     3901 ctctcaccac accgccgaag agctgtcgct ggtgcgcgaa ggcttctacg agaaacacgg
     3961 catcaaagtt ctggtcggcg aacgtgctat caccatcaac cgtcaggaga aggtgattca
     4021 ctccagcgcc ggacgtaccg ttttttatga caagctgatc atggcgaccg gttcctaccc
     4081 gtggatcccg ccaatcaaag gttctgatac tcaggactgc tttgtctatc gcaccattga
     4141 agacctcaac gccattgaat cctgcgcccg tcgcagcaag cgcggtgccg ttgttggtgg
     4201 cggcctgtta ggtctggaag ccgctggcgc gctgaaaaac ttaggtattg aaacccacgt
     4261 tatcgaattt gcccctatgc tgatggcgga acagcttgat cagatgggcg gcgagcagct
     4321 gcgtcgcaaa atcgagagca tgggcgtgcg cgttcacacc agcaaaaaca cccttgagat
     4381 tgtgcaggaa ggtgttgaag cgcgtaaaac catgcgtttt gccgacggca gcgaactgga
     4441 agtcgacttt atcgtcttct ctaccggtat ccgtccgcgc gataagctgg caacccagtg
     4501 tggtctggac gttgctccgc gtgggggtat cgtcattaat gattcctgcc agacttccga
     4561 tccggatatc tacgccatcg gtgaatgcgc aagctggaac aaccgtgtat ttggtctggt
     4621 agcaccaggc tacaaaatgg cgcaggtcgc cgttgaccat attctcggta gcgaaaacgc
     4681 ctttgaaggt gctgacctta gcgccaagct gaaactgctg ggcgtagacg taggcggtat
     4741 tggtgatgcg cacggtcgca caccaggcgc acgtagctac gtttacctcg acgaaagcaa
     4801 agagatctac aaacgcctta ttgtcagcga agacaacaaa accctgctcg gtgcggtact
     4861 ggtgggcgat accagcgact acggcaacct gctgcaactg gtgctgaatg ctatcgagct
     4921 gccggaaaac ccggattctc tgattctgcc agcacactcg ggtagcggta agccgtctat
     4981 cggtgttgat aaactgccgg acagcgcgca aatctgctcc tgcttcgacg tcaccaaagg
     5041 tgatctgatt gctgccatca acaaaggctg ccacaccgtt gcggcgctga aagccgaaac
     5101 caaagcgggt actggctgcg gtggctgtat cccgctggtc actcaggtac tgaacgcgga
     5161 actggcgaaa cagggcatcg aagttaacaa caacctgtgc gaacactttg cttattcgcg
     5221 tcaggaactg ttccatttga tccgcgttga aggcattaaa accttcgaag aactgctggc
     5281 gaaacacggc aaaggttacg gttgtgaagt ttgtaaaccg accgtcggtt cgctgctggc
     5341 ctcctgctgg aacgaataca ttctgaagcc ggaacatact ccgctgcagg attctaacga
     5401 caacttcctc gctaacatcc agaaagacgg cacctactcg gtgatcccgc gctctccggg
     5461 cggtgaaatc accccggaag ggctgatggc ggtaggacgt atcgcgcgtg aatttaatct
     5521 ctacaccaag atcactggct cccagcgtct ggcgatgttt ggcgcacaga aagacgatct
     5581 gccggagatc tggcgtcagc tgattgaagc cggcttcgaa accggtcatg cctatgcgaa
     5641 agcactgcgt atggcgaaaa cctgcgtggg tagcacctgg tgccgctacg gcgttggcga
     5701 cagcgtgggc ctcggcgtgg aactggaaaa ccgctacaaa ggcatccgta cgccgcacaa
     5761 aatgaagttc ggtgtctccg gctgtacccg tgaatgttca gaagctcagg gtaaagacgt
     5821 gggtattatc gccactgaaa aaggctggaa cctgtatgtt tgcggtaacg gcggcatgaa
     5881 accgcgtcat gcggatctgc tggcggcgga tatcgatcgc gaaacgctga tcaaatatct
     5941 cgaccgcttc atgatgttct acatccgtac tgccgacaaa ctgacgcgta ccgcaccgtg
     6001 gttagacaac ctcgaaggcg gcatcgatta cctgaaagcg gtgatcattg acgacaaact
     6061 ggggctgaac gcacatctgg aagaagagat ggcgcgcctg cgtgaagcgg tagtgtgtga
     6121 gtggactgaa acggtcaata cgccgtctgc gcagactcgc ttcaaacact tcatcaacag
     6181 cgacaaacgt gacccgaacg tacagatggt gccagagcgc gaacagcacc gtccggcaac
     6241 gccgtatgaa cgtatcccgg taactctggt ggaggacaac gcatgagcca gtggaaagac
     6301 atctgcaaaa tcgatgacat cctgcctgaa accggcgtct gcgcgctgtt aggtgacgag
     6361 caggtagcaa ttttccgccc gtatcacagc gaccaggtgt ttgcgatcag caacatcgac
     6421 ccgttcttcg agtccagcgt gctgtcacgc gggctgattg cggaacacca gggcgagctg
     6481 tgggtcgcca gcccgctgaa aaaacagcgt tttcgcttaa gtgatggctt gtgcatggaa
     6541 gacgaacagt tttccgtcaa acattacgaa gcgcgagtga aagacggcgt ggtgcagctg
     6601 cgcggttaat gttttaacgg gaggcgcaat gcctcccctt tttgcatggt cctgtaataa
     6661 tcttcggtat attgcaggac attttttaaa ctttttgttt tattttttgt ttttattttt
     6721 taaaggataa tcaaatgttt acagacacta ttaataagtg tgcggctaac gctgcgcgca
     6781 ttgcacgcct gtcggcaaat aacccactcg gcttttgggt cagctccgcc atggcgggcg
     6841 cgtatgtggg tcttgggatc atcctgattt tcacgctcgg taatttgctc gatccgtcca
     6901 tacgtcctct ggtgatgggc gcgacctttg gtatcgcctt aacgctggtg attatcgccg
     6961 gttctgaact gttcaccgga cacaccatgt tcctcacctt tggggtgaaa gcgggcacca
     7021 tcagccacgg gcaaatgtgg gcaatcctgc cgcaaacctg gttgggtaat ctggtcggtt
     7081 ccgtcttcgt tgccatgctc tatagctggg gtggtggtag cctgctgccg gtagatacca
     7141 gcatcgttca ctccgtcgcg ctggctaaaa ccactgcacc ggcaatggta ctgttcttca
     7201 aaggtgcatt gtgtaactgg ctggtttgtc tggcaatctg gatggcgctg cgcactgaag
     7261 gggcggcgaa atttatcgca atctggtggt gtctgctggc atttatcgcg tccggctacg
     7321 agcactccat cgctaacatg acgctgttcg cgctctcctg gttcggcaac cacagcgaag
     7381 cctacacgct ggcgggtatt ggtcataacc tgctgtgggt gacgctgggt aatactttat
     7441 cgggtgccgt attcatggga ttgggttatt ggtatgctac gccgaaagcg aatcgtccgg
     7501 ttgcggacaa atttaatcaa actgaaacgg ctgccggtta attactaagg ggtttttacg
     7561 tggatcattt gcctatattt tgccaattac gcgatcgcga ctgtctgatt gtcggcggtg
     7621 gtgatgtcgc ggaacgcaaa gcaaggttgc tgttagacgc aggcgctcgc ttaacggtga
     7681 atgcattagc gtttattcca cagttcaccg catgggcaga tgcaggcatg ttaaccctcg
     7741 tcgaagggcc atttgatgaa agccttctcg acacctgctg gctggcgatt gcagcgacgg
     7801 atgatgacgc gcttaaccag cgcgtcagcg aagccgctga agctcgtcgc atcttctgta
     7861 acgtggtcga tgcgccgaaa gccgccagct tcattatgcc gtcgattatt gaccgctcac
     7921 cgctgatggt ggcggtttcc tctggcggta cttctccggt tctggcgcgc ctgttgcgcg
     7981 aaaaactcga atcactgctg ccgctgcatc tgggccaggt agcgaaatac gccgggcagt
     8041 tacgcggtcg ggtaaaacaa cagttcgcca cgatgggtga acgccgccgc ttctgggaga
     8101 aattgttcgt taacgaccgc ctggcgcagt cgctggcaaa caacgatcag aaagccatta
     8161 ctgaaacgac cgaacagtta atcaacgaac cgctcgacca tcgcggtgaa gtagtgctgg
     8221 ttggcgcagg tccaggcgat gctggtcttc tgacgctgaa aggtctgcaa caaattcagc
     8281 aggcagatgt ggtggtctac gaccgtctgg tttctgacga tattatgaat ctggtacgac
     8341 gcgatgcgga tcgtgtcttc gtcggcaaac gcgcgggata tcactgcgta ccgcaggaag
     8401 agattaacca gatccttctt agggaagcgc aaaaaggcaa acgcgtggtg cgtctgaaag
     8461 gcggcgatcc gtttattttt ggccgtggtg gcgaagagct ggaaacactg tgtaacgcgg
     8521 gtattccgtt ctcggtggtt ccgggtatta ccgcagcttc tggttgttct gcctattcgg
     8581 gtattccgct cacgcatcgc gattatgccc agagcgtacg cttaattacc ggacacttaa
     8641 aaaccggtgg cgagctggac tgggaaaacc tggcggcaga aaaacagacg ctggtgtttt
     8701 atatggggct gaatcaggct gcgactattc agcaaaagct gattgaacac ggtatgcctg
     8761 gcgaaatgcc ggtagcaatt gtcgaaaacg gaacaacagt cacgcagcgc gtgattgacg
     8821 gtacgctcac acaactgggc gaactggcgc agcaaatgaa cagtccatcg ctaattatta
     8881 ttggtcgggt tgttggcctg cgcgataaac tgaactggtt ctccaaccat taatttaatc
     8941 agggccagag aattctggcc tttttcgttt acaactctct ttatatattc agcagataaa
     9001 aatataactc attaacaaaa atcatttgtt tttgccccgt tatattcacc gttacagcga
     9061 cttacaaaat taagtcattc actcactgac gccgattaaa tattcctgta tcgttctt
//
LOCUS       DAZUGK010000182         9039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZUGK010000182 DAZUGK010000000
VERSION     DAZUGK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.183"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2440
                     /locus_tag="ACHY5W_003776"
     CDS             <1..2440
                     /locus_tag="ACHY5W_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4448586.1"
                     /translation="ITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEV
                     EWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFA
                     FPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSIL
                     LVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNS
                     AFEHYFTADYYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEK
                     RCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATM
                     SHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESG
                     NYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSN
                     LLSNALKFTTEGAVKITTSMVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSA
                     GRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVATVEAKAEQ
                     PITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITD
                     VNMPNMDGFELTRKLREQKSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLK
                     THLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALE
                     AGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIA
                     ELDQEIAVFCQQND"
     gene            complement(2496..3641)
                     /gene="yfdE"
                     /locus_tag="ACHY5W_003777"
     CDS             complement(2496..3641)
                     /gene="yfdE"
                     /locus_tag="ACHY5W_003777"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFS4448587.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(3715..4659)
                     /gene="yfdV"
                     /locus_tag="ACHY5W_003778"
     CDS             complement(3715..4659)
                     /gene="yfdV"
                     /locus_tag="ACHY5W_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFS4448588.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(4729..6423)
                     /gene="oxc"
                     /locus_tag="ACHY5W_003779"
     CDS             complement(4729..6423)
                     /gene="oxc"
                     /locus_tag="ACHY5W_003779"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFS4448589.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6477..7727)
                     /gene="frc"
                     /locus_tag="ACHY5W_003780"
     CDS             complement(6477..7727)
                     /gene="frc"
                     /locus_tag="ACHY5W_003780"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFS4448590.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMEQNHAI"
     gene            complement(8240..8872)
                     /locus_tag="ACHY5W_003781"
     CDS             complement(8240..8872)
                     /locus_tag="ACHY5W_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFS4448591.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADTQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         2604 a         2084 c         1881 g         2470 t
ORIGIN      
        1 cataacaacg ccttacgttt ttgtcatgca aaaagcgcct gacagtgaac aaacattaaa
       61 aaaaggaatg aaagttgcca ttccatatta ttatgagctg cattcgcaat taaaagagat
      121 gtatccggag gtagagtgga taaaagtcga taacgccagc gctgcatttc acaaggtcaa
      181 ggaaggtgaa cttgatgctc tggtcgcgac acagctaaat tcgcgttaca tgatcgacca
      241 ttactatcct aatgaacttt atcattttct tattcccggc gttcagaatg catcactttc
      301 gttcgctttt cctcgcggag aaccggaact taaggatatt attaataaag cactgaatgc
      361 aattccccca agcgaagttc tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac
      421 cattgacaca tgggacctct atagcgagca attttatatt gttacgacat tatccatttt
      481 attagttggc agtagccttt tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa
      541 agtcattcag ggtgatttag aaaaccaaat atcattccgg aaagcgctct cggactcctt
      601 accgaatcca acttatgttg taaactggca aggtaatgtc attagtcaca atagtgcatt
      661 tgaacattat ttcactgctg attactacaa aaatgcaatg ttgccattag aaaacagtga
      721 atcacccttt aaagatgttt tttctaatac gcatgaagtc acagcagaga cgaaagaaaa
      781 ccgaacaata tacacacagg tatttgaaat tgataatggc atcgagaaaa gatgcattaa
      841 tcactggcat acattatgta atctgccagc cagcgaacat gctgtttata tttgtggttg
      901 gcaagatatt accgagacgc gtgatttaat tcatgcactc gaagtagaga gaaataaagc
      961 gatcaatgca actgtcgcaa aaagtcagtt tctggcaaca atgagtcacg aaataagaac
     1021 accaataagc tccattatgg gcttcctgga acttctgtcg ggttctggtc ttagcaagga
     1081 acaacgggtg gaggcgattt cacttgccta cgccaccgga caatcactcc tcggcttaat
     1141 tggtgaaatc cttgatgtcg acaaaattga atcgggtaac tatcaacttc aaccacaatg
     1201 ggtcgatatt cctactttag tccagaacac ttgtcactct ttcggtgcga ttgctgcaag
     1261 caaatcgatc gcattaagtt gcagcagtac gtttcctgaa cattacctgg ttaagatcga
     1321 ccctcaggcg tttaagcagg tcttatcaaa tttactgagt aatgctctca aatttaccac
     1381 cgagggggca gtaaaaatta cgacctccat ggttcacatt gatgacaacc acgctgtaat
     1441 caaaatgacg attatggatt ctggaagtgg attatcacag gaagaacaac aacaactgtt
     1501 taaacgttat agccaaacaa gtgcaggtcg ccagcaaaca ggttctggtt taggcttaat
     1561 gatctgcaaa gaattaatta aaaacatgca gggcgatttg tcattagaaa gtcatccagg
     1621 cataggaaca acatttacga tcacaatccc ggtagaaatt atccaacaag tggcgactgt
     1681 cgaggcaaaa gcagaacaac ccatcacact acctgaaaag ttgagcatat tgatcgcgga
     1741 tgatcatccg accaacaggc tattactcaa acgccagcta aatctattag gatatgatgt
     1801 tgatgaagcc actgatggtg tgcaagcgct acacaaagtc agtatgcaac attatgatct
     1861 gcttattact gacgttaata tgccgaatat ggatggtttt gagttgactc gcaaactccg
     1921 tgagcaaaaa tcttccttac ctatctgggg gcttacagcc aacgcacagg ctaacgaacg
     1981 tgaaaaaggg ttaaattgcg gcatgaactt atgtttgttc aaaccgttga ccctggatgt
     2041 actgaaaaca catttaagtc agttacacca ggttgcgcat attgcacctc agtatcgcca
     2101 ccttgatatc gaagccctga aaaataatac ggcgaacgat ctacaactga tgcaggagat
     2161 tctcatgact ttccagcatg aaacgcataa agatctaccc gctgcgtttc atgcactaga
     2221 agctggcgat aatagaactt tccatcagtg tattcatcgc atccacggtg cggctaacat
     2281 cctgaatttg caaaagttga ttaatattag ccatcagtta gaaataacac ctgtttcaga
     2341 tgacagtaag cctgaaattc ttcagctgct gaactctgta aaagaacaca ttgcagagct
     2401 ggaccaagag attgctgttt tctgtcagca aaatgactaa atagcggctc ccacaatgtt
     2461 caaatgtggg agctatttac cagcacatct tacatttatg atgagaactc ctggcgaatt
     2521 tgctccccat gctggtcgag cgttgccgct cccggcataa catgcgggtc cgcgcagcca
     2581 ctgattttta tcggattacc cggcatcatt attcccccgg cttcaatcaa catatttctc
     2641 gcctgagttt gtggcaaatt aatggcctca gccacactta ataacggcgc gacgggcaca
     2701 ccaacttcat gtattctggc taaccaaact tcagctgcct gtgtttttaa cgtccgctca
     2761 atatattgtt taagaatagc ctggttttgt acgcgtaaaa tattgctgct aaatctggga
     2821 tcattaacca gttccgtaag ctccagtgcc tggcataacg cagagaaaag cttgtcatta
     2881 ccacaacaaa tcgtaatcgg cttatcctga gtatcgaaaa catcaaaagg tgccatgtag
     2941 gggtggcgat ttcccaaacg ttgtggtgac ttccctgtcg caatatatgc catcagacca
     3001 tgctccagaa aactcagcgt ggcatcaaac atcgctatat cgacatgcgc ccctctctgg
     3061 cttttttcgc ggccataaag tgcactcact attccgctga ataaatagac accgccgcat
     3121 agatccgcaa gagaggtacc aacacgcact ggcggagcat cagggtaccc tgtttccatc
     3181 attatcccgc tcattgcctg aatgatggta tcgtaggcag gagcatcttt tagcggaccg
     3241 gtatgtccga aacctgacga tgaagcatat atgaggcgcg ggttgatttc ttgtaacctt
     3301 tcccatgaaa accccagttt ttccattgta cctgggcgaa aattctcagc taatacatca
     3361 gcttgtttaa gcatatttat aaatatactt ttatcgtgat cattctttaa atcaagaacc
     3421 acactctctt taccatgatt aataaaactg taatagagtg actgtccatc cacataggga
     3481 ccaaatgtgc gggtatcatc accatgacct ggtggctcaa ctttaattac ccttgcgccc
     3541 atattacaaa gaagttgagt tccgaaaggt ccattaagga catgtgtcat atcgataact
     3601 aataagcctt caaacggccc tttgctttca ttatttgtca tttgcttatg ccttgaaaaa
     3661 agccgaaggt gaaaagacac ttcggcattt atgtaaaatc aataacatga ttaattatga
     3721 aaccagtcga ctgacataaa tccacaaggg agccgtgacg acaaatccca gtacgctcac
     3781 agccaatgac gctgtaccag tgcgggtgta gacattaaac cggctggcaa taatgattcc
     3841 ggagaatgcc ggcggtaatg cgcctgccag taccatcatc tgcagatgtt cgctgttcaa
     3901 atgacatgcc ataccaacaa gaagcagcgc cagtggcatc agaatcagct tcaggaaggt
     3961 gttataagca atttcggcac tgaactcgaa tttatgtgca gccagagtca accccgcagc
     4021 gaataccgct acccctgagt tagccttcgc aatcagatta aaggttgggt cccatgctgc
     4081 cggaattttt accccaacca gcaccaggat cgttgccaga acaggtgccc atactacagg
     4141 ctcctttgcc gcagaaatta atgcgctcag attactattc ttcttaccat ccgctcctga
     4201 ggaaggattc agcaaataca gaccaatagg aatagtaatt gcgttaacaa taatagaaat
     4261 aattgctact actaaacctg ttgatacgga atcaccataa ataggatcga gaactgcaaa
     4321 tcccaggaat ccaatggtag gtgaaccagc aattaatgca catacagctg cttctgcatg
     4381 ggtacgttta aaaaatttgt agcaaccaaa ccaggagaaa aagaaacatc cgacaataac
     4441 cacaagtgat acaagggtca gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt
     4501 aatagaaaca aatagagccg caggaagagc gtagtttaat accagtttat taaatgcccg
     4561 ggcttgatct tctgaaaaag tttctcgtct gccgctaaaa taacccaaca gcatgattac
     4621 gataataggc aataaatcgc caataaaaaa tgttagcata atagtatttc cgataagggt
     4681 cccctcacct tcgcataatg agttggcaag aggagacgat gtcagtattt aattaccagc
     4741 gacttgtttt gggttaagtt tggtaatatg gccactttca gttcctgctg cagggtcgat
     4801 gaccacatta ataatggtcg gtttgcgcga ctgaataccg gtggttaaag catgacgaag
     4861 ttcatctgtc gtggtgacgt tatagccaac accacgaaac gcatccatta atttgtcata
     4921 ccttgcatgg tgcaacagat ctgttggtga tggtgcacca gcgccactga gatcaacacc
     4981 gtctcctctg tagatgccgc cattattaaa aataacgatc gtcaccggca ggttatatcg
     5041 acaaatcgtt tcaatttcca tcccactgaa accaaaagca ctatcacctt caatggcgac
     5101 aaccggagaa ccagaggtca cgctagcacc gatggcatac cccataccga tgcccatgac
     5161 accccaggta ccacaatcca gacgacgacg tggtttatac atatcaataa tatttcgtgc
     5221 attatccagg gtatttgcac cttcattaac taaataaata tcctggttct cacgcaatac
     5281 atcgcgcaca gcacttaatg cattaaagta atttaatggt tgggtatctg tacttaattt
     5341 ttcatgcatt ttttgtgcat tttgctgctt gtgaatattt aaaatatcgc gccataccag
     5401 tggagtcgta aatgtgtttt gtttcagttc tgccagcata ccttgcatac tggatgcaat
     5461 atcgccaacg actggcacag caatggggcg gttgctgtca atttcctgcg gttcaatatc
     5521 cagttgaata aactgtgtat ctgccgccca tcctttttta ccgtgtgcca gtaaccaatt
     5581 cagtcgtgca ccaacaagca tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc
     5641 tgccgcagaa agtggatgcg tatcttcgag gatccctttc gccatagaca ttggcaggaa
     5701 tggaatctgg gcactttcaa taaattcacg aagctgttcg tcagcttgtg aatacgccgc
     5761 gcctttgcca aggataatta atggccgttc agcttttgct aaaagcgaaa ttgcgctagt
     5821 gactgacttc gggcatggca ataatgctgg cgacggattt tcaactttta caatcgtggt
     5881 taacgcttcg tctttttcca tcgtcgcggc caggacattt gctggcaaat caagataaac
     5941 tccgccaggg cgacccgata ctgagactcg gatagcgcgt gccaatgcaa tgccaagatc
     6001 ctgcggctga ttaacgcgaa atgctgcttt ggcatacggt tttgccgcat tcatttggtc
     6061 cagctcttca taatcacctt gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat
     6121 catgatcatc ggaaaaccat ttaccgttgc gttggccaat gcggtcaaac cattgaggaa
     6181 tcctggcgca gacactgtca ggcagatccc tggtttttgg gtaagaaaac cgcttgccgc
     6241 agcggcatag cctgccgact gctcatgacg aaaaccaata taacgaatgc cttccgcctg
     6301 ggcatggcgc gccatatccg tcacaggaat acctacaaca ccataaatag tgtcaatatt
     6361 attctgtttt aatgcttcaa cgatgatatg cataccatct gtcatttgaa gttgatctga
     6421 catgattaat aacctcgaaa tttttataaa ttgggagcca gaagcccccg ttaatatcag
     6481 atggcgtggt tttgctccat tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat
     6541 acagcagcgg tatgttcacc taatagcggc gcagctttaa tatctggcgt aaaggcagag
     6601 aatttcattg gacaaccaac tgtcagatat tttccacgca acggttgttc cacttcgaca
     6661 acactgccac tttggcgtaa agagggatca agtgaaattt ctttcatact taaaaccggt
     6721 gcacaaggaa tatcaaactg agtcaaatag gccaccgctt catgtttatc aatagtgaca
     6781 gtgtattttt cgatttcagc aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta
     6841 ctgtatgccg gatcggtaat ccaatctggt ttgccgatgg ctttacaggt gttttcccag
     6901 ttttgctcct gaatagtgaa ataaatatag gcgttaggat ctgtttccca gcctttacat
     6961 ttcaggatcc atccaggttg accgccacca cccgcattac caccacgggg aactgcatca
     7021 ccaaatgtac cattcggata ctgcgggtat tcttccagat aacccaattt atcgagacgc
     7081 tgctggtcgc gtaatttcac gcggcaaagg ttcaatacgg catcctgcat tgacatggtg
     7141 actcgttgcc cacgccccgt tttttcgcga tgcagcaaag cagcaagtaa accgatcagc
     7201 aaatgcattc cggtgttgct gtctcctaac gctgcagcgc ttaccagcgg cggaccgtcc
     7261 caaaaacccg tagtggatgc cgcgccaccc gctgcctgag caacgttttc ataggctttt
     7321 acattcacat aaggcgaaca ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga
     7381 ttgatttctt gaatatgctc ccaggtgaaa cccatgtgat caatggcccc tggatgaaag
     7441 ttctcgacta agatatcagc ttcgcggatc agcttttcca ttacctcttt gccttccgct
     7501 gttttggtat ttaactcaat agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca
     7561 tcgatatcag gaatatctcg cagctggtga cgcgttacgt caccaacgcc gggacgttca
     7621 attttaataa cgtcagcgcc aaaccaggcc agcatttgag tacaagatgg gccagattgc
     7681 acaccggtga aatcgagaac tttaattcct tgaagtggag ttgacatagc aatacctatt
     7741 agttaattaa atatgtgtta atttagcgtt tatttaaggc ataacgccct ctataaataa
     7801 tccgcaaaat atcttcgtag cggcactcga tattttcaca ttcctgtatc aagtgctgaa
     7861 aatcatttca tcgctacttc caattccttt ctgcatggta agtagtgagg acgaaaagaa
     7921 gaatatgata aatattggtt cgtggtaatc agagagagtc gcgacatttg tgatgttatt
     7981 cttacaactg taagaagcgc cggtgattta caagtaaggg tatgattacg ctttttaaag
     8041 cgtaccataa aatataacat attaaatgat ttgtgatgac acgggattat tgcggtttac
     8101 tttgaggaat ataaaatgcg agtttataga accgcgagcg ccaaataatt ttatgaccac
     8161 aacctgtcgt attgtggtca taaaaaattg tcggctggag ggagttaaat cctttacctc
     8221 aatatactgc ctgtcagcat tagttcacaa acagtgcttc gctatcgacg atgattccat
     8281 cttccgcacc tttaagcgcc aggtttgcct cgtagtattg ctttttatca agcagtttct
     8341 gcgtatcagc aagtgcatta cgagtctgtt taagcggcat cagatattgg ttttccatta
     8401 caccaacacc ggccaggcga agctcttcca ttgcgccctt tttatcccct ttcgccattt
     8461 tttcattggc aatttttatc gcggcttctt tttctggtgt cgctacataa ctctcagaaa
     8521 ttccgacaga tgcattaatg acaatatatt ggtcatcatt gagattggtc tttttacctg
     8581 gcttagcgaa ctttgcccat tcggtactgt catctgacaa caaagcggaa gcttcattgg
     8641 tcagttcttt tgccttttcc ggatcgccat gaaacagagc caggcgcgct acctgcacgt
     8701 cacgcatcgc atataaacct tgttcagaga tacgctctgc tgctgccgtt ttctgggttt
     8761 gctgaacttg ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag
     8821 ttgaagatgc cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct
     8881 cattctttaa cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat
     8941 cagagagcaa cggtcatttt gaaagggtta tcttacagtt gtagttatta tcgcaagaag
     9001 tattttagtc ttacaatcgt aggaatatgc ttcctgtca
//
LOCUS       DAZUGK010000183         8773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZUGK010000183 DAZUGK010000000
VERSION     DAZUGK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.184"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(4..630)
                     /gene="upp"
                     /locus_tag="ACHY5W_003782"
     CDS             complement(4..630)
                     /gene="upp"
                     /locus_tag="ACHY5W_003782"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4448592.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            955..1992
                     /gene="purM"
                     /locus_tag="ACHY5W_003783"
     CDS             955..1992
                     /gene="purM"
                     /locus_tag="ACHY5W_003783"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4448593.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1992..2630
                     /gene="purN"
                     /locus_tag="ACHY5W_003784"
     CDS             1992..2630
                     /gene="purN"
                     /locus_tag="ACHY5W_003784"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4448594.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2802..4868
                     /gene="ppk1"
                     /locus_tag="ACHY5W_003785"
     CDS             2802..4868
                     /gene="ppk1"
                     /locus_tag="ACHY5W_003785"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFS4448595.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4873..6414
                     /gene="ppx"
                     /locus_tag="ACHY5W_003786"
     CDS             4873..6414
                     /gene="ppx"
                     /locus_tag="ACHY5W_003786"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFS4448596.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMELGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6453..8696)
                     /gene="pdeF"
                     /locus_tag="ACHY5W_003787"
     CDS             complement(6453..8696)
                     /gene="pdeF"
                     /locus_tag="ACHY5W_003787"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFS4448597.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETISLTVNFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLVLFQFAAFVGLLASRTNLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2265 a         2159 c         2186 g         2163 t
ORIGIN      
        1 tctttatttc gtaccaaaga ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc
       61 gtgctcgttc agtccctgat caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt
      121 ttccagcgca gcgatacctt ctggcgcagc taccagcacc agaactttga tgctgctgca
      181 gcccgctttt ttcagcaggt cgatggtcgc gataacggaa ccaccggtcg ccagcattgg
      241 gtcaacgatc agcgccatac gctcatcgat gttagaaacc agtttctgga agtacggtac
      301 cggctccagc gtttcttcat tacggtacat accgacaacg ctgatgcgcg cgctcggaac
      361 gttttccagc acaccgtcca tcataccaag acccgcacgc agaattggca caacggtaat
      421 tttcttacct ttgatctggt cgatttctac cgggccgttc cagccttcga tagttacttt
      481 ttccgtttcg aggtcggcgg tcgcttcgta agtcagcagg ctacccactt cggaagcgag
      541 ttcgcgaaag cgcttggtgc tgatatcttg ctcacgcatc agtcccagct tgtgtttgac
      601 gagtgggtgt tttacttcca cgatcttcat actctttctc ctttgagggg cagccacaaa
      661 aaaaatcgac ggattatacc tcctttcttc aaggaggcaa tattattttc gttgacttta
      721 gtcaaaatga taacggttag agataaagtt attttatatt caaatggtta tgaaagaaga
      781 ttattccatc cgaaaactaa cctttgccct ggcacaagtc ttctttcgcc gcgcgcctgg
      841 ggaaaagacg tgcaaaaagg ttgtgtaaag cagtctcgca aacgtttgct ttccctgtta
      901 gaattgcgcc gaattttatt tttctaccgc aagtaacgcg tggggaccca agcagtgacc
      961 gataaaacct ctcttagcta caaagatgcc ggtgttgata ttgacgcggg taatgctctg
     1021 gttggaagaa tcaaaggcgt agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg
     1081 ggcggcttcg gtgcgctgtg tgcattgccg caaaaatatc gtgaacccgt gctggtttcc
     1141 ggcactgacg gcgtaggtac caagctgcgt ctggcaatgg acttaaaacg tcacgacacc
     1201 attggtattg atctggtcgc catgtgcgtt aatgacctgg tggtgcaagg tgcggaaccg
     1261 ctgtttttcc tcgactatta cgcaaccgga aaactggatg ttgataccgc ttcagcggtg
     1321 atcagcggca tcgcggaagg ttgtctgcaa tcgggctgtt ctctggtggg tggcgaaacg
     1381 gcagaaatgc cggggatgta tcacggtgaa gattacgatg tcgcgggttt ctgcgtgggc
     1441 gtggtagaaa aatcagaaat catcgacggc tctaaagtca gcgacggcga tgtgctgatt
     1501 gcactcggtt ccagcggtcc gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa
     1561 gtcagcggtt gtgatccgca aaccaccgaa cttgatggta agccattagc cgatcatctg
     1621 ctggcaccga cccgcattta cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg
     1681 catgccattg cgcacctgac cggcggcggc ttctgggaaa acattccgcg cgtattgcca
     1741 gataataccc aggcagtgat tgatgaatct tcctggcagt ggccggaagt gttcaactgg
     1801 ctgcaaacgg caggtaacgt tgaacgccat gaaatgtatc gcaccttcaa ctgcggcgtc
     1861 ggcatgatta ttgccctgcc tgctccggaa gtggacaaag ccctcgccct gctcaatgcc
     1921 aacggtgaaa acgcgtggaa aatcggtatc atcaaagcct ctgattccga acaacgcgtg
     1981 gttatcgaat aatgaatatt gtggtgctta tttccggcaa cggaagtaat ttacaggcaa
     2041 ttattgacgc ctgtaaaacc aacaaaatta aaggcaccgt acgggcagtt ttcagcaata
     2101 aggccgacgc gttcggcctt gaacgcgccc gccaggcggg tattgcaacg catacgctca
     2161 tcgccagcgc gtttgacagt cgtgaagcct atgaccggga gttgattcat gaaatcgaca
     2221 tgtacgcacc cgatgtggtc gtgctggctg gttttatgcg cattctcagc ccggcgtttg
     2281 tctcccacta tgccgggcgt ttgctgaaca ttcacccttc tctgctgccg aaatatcccg
     2341 gattacacac ccatcgtcag gcgctggaaa atggcgatga agagcacggt acatcggtgc
     2401 atttcgtcac cgatgaactg gacggtggcc cggttatttt acaggcgaaa gtcccggtat
     2461 ttgctggtga tacggaagat gacgtcaccg cccgcgtgca aacccaggaa cacgccattt
     2521 atccactggt gattagctgg tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt
     2581 ggctggatgg tcaacgtctg ccgccgcagg gctacgctgc cgacgagtaa tgcccccgta
     2641 gttaaagcgc cagctctgcc gctggcgttt ttcaattcac ctgtaaatcg caagctccag
     2701 cagttttttt cccccttttc tggcatagtt ggacatctgc caatattgct cgccataata
     2761 tccaggcagt gtcccgtgaa taaaacggag taaaagtggt aatgggtcag gaaaagctat
     2821 acatcgaaaa agagctcagt tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg
     2881 acaaatctaa cccgctgatt gaaaggatgc gtttcctggg gatctattcc aataaccttg
     2941 atgagttcta taaagtccgc ttcgctgaac tgaagcgacg catcattatt agcgaagaac
     3001 aaggctccaa ctctcattcc cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag
     3061 ccgatcagga attcgacggc ctctacaacg agctattgct ggagatggcg cgcaaccaga
     3121 tcttcctgat taatgaacgc cagctctccg tcaatcaaca aaactggctg cgtcattatt
     3181 ttaagcagta tctgcgtcag cacattacgc cgattttaat caatcctgac actgacttag
     3241 tgcagttcct gaaagatgat tacacctatc tggcggtgga aattatccgt ggcgatacca
     3301 tccgttacgc gctgctggag atcccatcag ataaagtgcc gcgctttgtg aatttaccgc
     3361 cagaagcacc gcgtcgacgc aagccgatga ttcttctgga taacattctg cgttactgcc
     3421 ttgatgatat tttcaaaggc ttctttgatt atgacgcgct gaatgcctat tcaatgaaga
     3481 tgacccgcga tgccgaatac gatttagtgc atgagatgga agccagcctg atggagttga
     3541 tgtcttccag tctcaagcag cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg
     3601 atatgcccaa tgcgctggtt gaagtgttac gcgaaaaact gactatttcc cgctacgact
     3661 ccatcgtccc cggcggtcgt tatcataatt ttaaagactt tattaatttc cccaatgtcg
     3721 gcaaagccaa tctggtgaac aaaccactgc cgcgtttacg ccatatttgg tttgataaag
     3781 cccagttccg caatggtttt gatgccattc gcgaacgcga tgtgttgctc tattatcctt
     3841 atcatacctt tgagcatgtg ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac
     3901 tggcgattaa aattaacatt taccgcgtgg cgaaagattc acgcatcatc gactcgatga
     3961 tccacgctgc acacaacggt aagaaagtca ccgtggtggt tgagttacag gcgcgtttcg
     4021 acgaagaagc caacattcac tgggcgaagc gcctgaccga agcaggcgtg cacgttatct
     4081 tctctgcgcc ggggctgaaa attcacgcca aactgttcct gatttcacgt aaagaaaacg
     4141 gtgaagtggt gcgttatgca cacatcggga ccgggaactt taacgaaaaa accgcgcgtc
     4201 tttatactga ctattcgttg ctgaccgcag atgcgcgcat cactaacgaa gtacggcggg
     4261 tgttcaactt tattgaaaac ccataccgtc cggtgacatt tgattattta atggtgtcgc
     4321 cgcaaaactc ccgccgcctg ttgtatgaaa tggtggaccg cgagatcgcc aacgcgcagc
     4381 aagggctgcc cagtggtatc accctgaagc taaataacct tgtcgataaa ggcctggttg
     4441 atcgtctgta tgcggcctcc agctccggcg taccggttaa tctgctggtt cgcggaatgt
     4501 gttcgctgat ccccaatctg gaaggcatta gcgacaacat tcgtgccatc agtattgttg
     4561 accgttacct tgaacatgac cgggtttata tttttgaaaa tggcggcgat aaaaaggtct
     4621 acctttcttc tgccgactgg atgacgcgca atattgatta tcgtattgaa gtggcgacgc
     4681 cgctgctcga tccgcgcctg aagcagcggg tgctggacat catcgacata ttgttcagcg
     4741 atacggtcaa agcacgttat atcgataaag aactcagtaa tcgctacgtt ccccgcggca
     4801 atcgccgcaa agtacgggcg cagttggcga tttatgacta catcaaatca ctcgaacaac
     4861 ctgaataacc ctatgccaat acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc
     4921 gatcttggtt caaacagttt tcacatggtc atagcccgtg tggtagatgg tgccatgcag
     4981 attattggcc gcctgaaaca gcgggtgcat ctggcggacg gcctggggcc agataatatg
     5041 ttgagtgaag aggcaatgac gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta
     5101 caagggtttt ctcctgccag cgtctgtata gttggtaccc atacgctgcg tcaggcgctg
     5161 aacgccactg actttctgaa acgtgcggaa aaggtcattc cctacccgat tgaaattatt
     5221 tccggtaatg aagaagcccg tctgattttt atgggcgtgg aacataccca accggaaaaa
     5281 ggtcgcaaac tggttattga tattggcggc ggttctacag aactggtgat tggtgaaaat
     5341 ttcgaaccga ttctcgttga aagccgccgg atggggtgtg tcagctttgc ccagctttat
     5401 ttccctggcg gagtcatcaa taaagagaat tttcagcgtg ctcgcatggc ggcagcacaa
     5461 aaactggaaa ctttaacctg gcaatttcga attcaaggct ggaacgtggc aatgggcgct
     5521 tccggtacca taaaagccgc ccatgaagtg ttaatggaat tgggtgagaa agacgggata
     5581 attaccccgg aacgtctgga aaaactggta aaagaagttt tacggcaccg taatttcgca
     5641 tcgctgagtt taccaggtct ttccgaagag cggaaaacag tcttcgttcc gggactggcg
     5701 attttatgcg gtgtgtttga tgctttagcc atccgtgaac tgcgcctttc tgacggggcg
     5761 ttacgcgaag gcgtactgta tgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt
     5821 cgtaccgcca gcagcctcgc caaccagtat cacatcgaca gcgagcaggc ccgacgggtg
     5881 ctggatacca ctatgcaaat gtacgaacag tggcgggaac agcaaccgaa gctggcgcat
     5941 ccgcaactgg aggcgctact gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc
     6001 aaccacagcg gtttgcatcg ccactccgct tatattctgc aaaacagtga cttgccgggt
     6061 tttaatcagg aacagcagct gatgatggcg acactggtgc gctatcaccg taaagcgatt
     6121 aagctcgacg atctgccgcg ctttaccttg tttaagaaga aacagttcct gccactgata
     6181 cagctattgc gccttggcgt attactcaac aatcagcgtc aggcaaccac cacaccgcca
     6241 acattgacgc tgattactga tgacagtcac tggacactgc gtttcccgca tgactggttt
     6301 agtcagaatg cgctggtact gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg
     6361 gctggctggc ggttgaaaat tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt
     6421 gcgggccgac gagaaatgtc ggcccgcatt attcaggcac tttcgcgaat gggttcgatt
     6481 tcattcagcg tatcaattaa cggttgcggc ttaccaataa gataaccctg catataatcg
     6541 atccccaaag agagcactgc ctcgcggatc tcttcgtttt caacgtactc tgccactacc
     6601 agcattttct tcattcgcgc caggtggcaa atcgacgcca ctatctgata atccagacta
     6661 tttgacacaa tattgcggat aaaactgccg tcaattttaa gcagatcggc attcacattt
     6721 ttaagccgcg catagctggc gtagccggtg ccgaaatcat caatcgcaat ctggcagcct
     6781 aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa cattggtcag agcattactt
     6841 tcggtgactt caaaaataag ttgccacgct tcaatctgat atttagccag caactgactg
     6901 acttcaacag gaaaacgagc ctgacatacc gaggttggag acagattaat agcaaaacgg
     6961 tgagcgggca tcttcgctct gttttcagcc ataaattgca gcgtatgctc aatgacccac
     7021 atgtcgatac tcgacgataa accaaattcg tgcgcgaccg gcaagaagct atcggggctg
     7081 atcagttcat cattctcacc tttcatgcgc agaagaattt catggtaaac atcaccacgc
     7141 ataccggtaa tcggctgggc catcaggaaa aaatggttgt gttccagcgc ctgctgtagt
     7201 cgattcatca tcgcgacttt atctttcaat tcgcgttgca aatacattgc cccgcgacgc
     7261 tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc
     7321 agcagcaggt agatatgatt cactggcgag cgcacatagc agtaactgac gccaatctgc
     7381 ggttgcatcg gcatgccatc ccaaaagaaa cgaaattgct tgagatggct atccagtgcg
     7441 gtaatacgct cctggtgcga ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc
     7501 tgataaacat cttcacccgg ttccagcaag ggtgacagcc agtgagaaag tttttgcttg
     7561 tattgaatcc gcagcatgat gccatagttt ttaaccagca tttccatgcc agggatgcgt
     7621 aaataacaaa gcgcagacca gggggcatca cgcaacgcgc gattcagggc gcgaacattt
     7681 ggcagatgaa ccaccgggtc cacatacgca agccgctgaa tgcgtctgac taccgttctc
     7741 tgacgggttg ccagtacagc catataattg acaataaaag agaataccag ataactggag
     7801 gaggttatgg tcagctgcgt ggtatagcca ggataaatgg gaatgtaatt ttgatagctg
     7861 tggatgctga tcatcaacac gaccgcccag agcagtgaaa tcagcttata accatagcgc
     7921 atcgctcccc acatcatcag gggcagcaat aatgacaagg tataattggt gctaaaaatt
     7981 gtgctttttt catttaacgg catgcataac agcaacagta aagcacctaa tgccagtagc
     8041 cagagtgcga actctttttt ggtgactttg acatcaacct gctgttttaa ttgcgaataa
     8101 taactacgta aataaaacgg atttcgaact acccgaatga tgaagtagca cagcgggaca
     8161 ccaatcagat tgcccaccag taaggcctga taattgatta aggttcccag attaaagggc
     8221 atgacaccaa ccagatttgt tctgctcgcc agtaaaccta caaacgccgc aaactggaag
     8281 agcaccagaa acaacgttgc agggaaaaca atctgccaaa aaatgcgctg ggaaattaag
     8341 cgggtatcac catgtgaaac gttgttacgc cggggggtaa acaccctata gcccccccaa
     8401 caaagaatga taatgataat gaaattaacc gttaacgaga tcgtttcata aaaaccaagt
     8461 tcggcatact tgcgaacaaa aatccctaaa gcaatacctg gcaatgctgc ccagctaaaa
     8521 aacatcatca tgctgatcat cagtgccaaa ggcagataaa agagaaaaac ctcaccggaa
     8581 gaaatatgcg cgaaagtatt aatgtgggcg aaaatgggca agattaaaga aggcaggaac
     8641 agcggaagcc cccaccattt atcacgtatt tttatataag ttgcattcag tttcatagat
     8701 gctcagcaga atcccccaca tcctgaagga ggtgtattca gacaggcatc ccacctgact
     8761 tcgaatgatg att
//
LOCUS       DAZUGK010000184         8704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZUGK010000184 DAZUGK010000000
VERSION     DAZUGK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.185"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..811)
                     /gene="dsbG"
                     /locus_tag="ACHY5W_003788"
     CDS             complement(65..811)
                     /gene="dsbG"
                     /locus_tag="ACHY5W_003788"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFS4448598.1"
                     /translation="MLKKILLLALLPAIAFAEELPDPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENVSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLEANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1183..1746
                     /gene="ahpC"
                     /locus_tag="ACHY5W_003789"
     CDS             1183..1746
                     /gene="ahpC"
                     /locus_tag="ACHY5W_003789"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4448599.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1917..3482
                     /gene="ahpF"
                     /locus_tag="ACHY5W_003790"
     CDS             1917..3482
                     /gene="ahpF"
                     /locus_tag="ACHY5W_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4448600.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3603..4031)
                     /gene="uspG"
                     /locus_tag="ACHY5W_003791"
     CDS             complement(3603..4031)
                     /gene="uspG"
                     /locus_tag="ACHY5W_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFS4448601.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4252..5490
                     /gene="ybdR"
                     /locus_tag="ACHY5W_003792"
     CDS             4252..5490
                     /gene="ybdR"
                     /locus_tag="ACHY5W_003792"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFS4448602.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5494..5682)
                     /gene="yldA"
                     /locus_tag="ACHY5W_003793"
     CDS             complement(5494..5682)
                     /gene="yldA"
                     /locus_tag="ACHY5W_003793"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFS4448603.1"
                     /translation="MHSHPLYAKSAIFPGSHCSLLLQNYSVSFVGITMAEAFYILIGF
                     LIMAAIIVMAVLYLENHS"
     gene            complement(5721..6131)
                     /gene="rnk"
                     /locus_tag="ACHY5W_003794"
     CDS             complement(5721..6131)
                     /gene="rnk"
                     /locus_tag="ACHY5W_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFS4448604.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6360..7166)
                     /gene="rna"
                     /locus_tag="ACHY5W_003795"
     CDS             complement(6360..7166)
                     /gene="rna"
                     /locus_tag="ACHY5W_003795"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFS4448605.1"
                     /translation="MKAFWRNASLLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAIKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(7280..>8704)
                     /gene="citT"
                     /locus_tag="ACHY5W_003796"
     CDS             complement(7280..>8704)
                     /gene="citT"
                     /locus_tag="ACHY5W_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFS4448606.1"
                     /translation="PLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATA
                     ISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFAL
                     GYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKN
                     LPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQI
                     SWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWASDELKTMGALTRREWT
                     LIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLAT
                     LVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATM
                     LPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRL
                     GAIFGVIYISMLLLVGWPILAMWN"
BASE COUNT         2266 a         2138 c         2205 g         2095 t
ORIGIN      
        1 aattcatgct tttttaggat ggttgaaaag cagggatata gttcaaccat ccatgaatta
       61 ccttttattt atttcccata atgatattaa gcgttttctg atcgggcaac cccacggcct
      121 gttgcagcgt attttcctta ctcatgtaat agatagccgg agtgacattt gcctccagat
      181 cgtccatcag tttctcgttg tcgcttaaca ctttcatttg ctctgtactt acgtttgcag
      241 gcacgtttag cttaagcttg ccaccagagg cttcatattc ttgccaggtt ttcgcgggat
      301 ctttggaggc aagaattgcc gcggctgtcg ccggactttc tggtttgata accccaacca
      361 acaatgttct caactgtact ttgccagaat ctacccacgg gcgcgcctgc tgccagaact
      421 gtttacaata tgggcagaac ggatcggcga agacgtagac aatgaccggc gcgtcttttt
      481 taccgtcgag gagccagtgg gattgttcca tccgttgcca catttcgcgt ccggctggtg
      541 cgtaaatttc tttttcgata agtgtgttac tcacgttttc acctttctcg ttgtacatgt
      601 aaccagagat agcgtgctta ccatctggag tcaggtagat ggtgacgccc atatcctgat
      661 actttccgag ataacctttc attcctccgg gggcatcgaa cgttttgatg atcgtaatcc
      721 cctgtttttc aatcgctttt actggatcag gaagttcctc tgcgaaggcg attgcaggaa
      781 gcagagccag taaaagtatc ttttttaaca ttaatttgtc cttttcagtc agtgcaaaag
      841 tcgagtaaaa ggcataacct atcattgtca taggtaagag cttagatcag gtgattgccc
      901 tttgtttatg agggtgttgt aatccatgtc gttgttgcat ttgtaagagc aacaccgcag
      961 cctgcagaca ggcgctgaag ataccaaagg gtagttcaga ttacacggtc acctggaaag
     1021 ggggccattt tactttttac cgccgctggc ggtgcaaagt tcacaaagtt gtcttacgaa
     1081 ggttgtaagg taaaacttat cgatttgata atggaaacgc attagccgaa tcggcaaaaa
     1141 ttggttacct tacatctcat cgaaaacacg gaggaagtat agatgtcctt gattaacacc
     1201 aaaattaaac cttttaaaaa ccaggcattc aaaaacggcg aattcatcga aatcaccgaa
     1261 aaagataccg aaggccgctg gagcgtcttc ttcttctacc cggctgactt tactttcgta
     1321 tgcccgaccg aactgggtga cgttgctgac cactacgaag aactacagaa actgggcgta
     1381 gacgtatacg cagtatctac cgatactcac ttcacccaca aagcatggca cagcagctct
     1441 gaaaccatcg ctaaaatcaa atatgcgatg atcggcgacc cgactggcgc cctgacccgt
     1501 aacttcgaca acatgcgtga agatgaaggt ctggctgacc gtgcgacctt cgttgttgac
     1561 ccgcagggta tcatccaggc aatcgaagtt accgctgaag gcattggccg tgacgcgtca
     1621 gacctgctgc gtaaaatcaa agcagcacag tacgtagctt ctcacccagg tgaagtttgc
     1681 ccggctaaat ggaaagaagg tgaagcaact ctggctccgt ctctggacct ggttggtaaa
     1741 atctaagttt ccttcgtctt tcacgctacc aactccggcg cgaaatactt cggaaattca
     1801 cctaattctt cgggtgctgc ggcacccgat tttcttcccc gcaccatgat gcaagctgca
     1861 tccaggtagc cgtagaggcc gcttgcatga tgatgtttaa agcccaggag ataaacatgc
     1921 tcgacacaaa tatgaaaact caactcaagg cttaccttga gaaattgacc aagcctgttg
     1981 agttaattgc cacgctggat gacagcgcta aatcggcaga aatcaaggaa ctgttggctg
     2041 aaatcgcaga actgtcagac aaagtcacct ttaaagaaga taacagcttg ccggtgcgta
     2101 agccgtcttt cctgatcacc aacccaggtt ccaaccaggg gccacgtttt gcaggctccc
     2161 cgctgggcca cgagttcacc tcgttggtac tggcgttgct gtggacaggc ggtcatccgt
     2221 cgaaagaagc gcagtctctg ctggagcaga ttcgccatat tgacggtgat tttgaattcg
     2281 aaacctatta ctcgctctct tgccacaact gcccggacgt ggtgcaggcg ctgaacctga
     2341 tgagcgtatt gaacccgcgc atcaagcaca ctgcaattga cggcggcacc ttccagaacg
     2401 aaatcaccga tcgcaacgtg atgggcgttc cggcagtgtt cgtaaacggg aaagagtttg
     2461 gtcagggccg catgacgttg actgaaatcg ttgccaaaat tgatactggc gcggaaaaac
     2521 gtgcggcaga agagctgaac aagcgtgatg cttatgacgt attaatcgtt ggttccggcc
     2581 cggcgggtgc agcggcagca atttactccg cacgtaaagg catccgtacc ggtctgatgg
     2641 gcgaacgttt tggtggtcag atcctcgata ccgttgatat cgaaaactac atttctgtac
     2701 cgaagaccga aggccagaaa ctggcaggtg cgctgaaagt tcacgttgat gaatacgacg
     2761 ttgatgtgat cgacagccag agcgccagca aactgatccc ggcagcggtt gaaggtggtc
     2821 tacaccagat tgaaacagct tctggcgcgg tactgaaagc acgcagcatt atcgtggcga
     2881 cgggtgcaaa atggcgcaac atgaacgttc cgggcgaaga tcagtatcgc accaaaggcg
     2941 tgacctactg cccgcactgc gacggcccgc tgtttaaagg caaacgcgta gcggttatcg
     3001 gcggcggtaa ctccggcgtg gaagcggcaa tcgacctggc gggtatcgtt gagcacgtaa
     3061 ctctgctgga atttgcgcca gaaatgaaag ccgaccaggt gctgcaggac aaactgcgca
     3121 gcctgaaaaa cgtcgacatt attctgaatg cgcaaaccac ggaagtgaaa ggcgacggca
     3181 gcaaagtcgt tggtctggaa tatcgagatc gtgtcagcgg cgatattcac aacatcgaac
     3241 tggccggtat tttcgtccag attggtctgc tgccgaacac caactggctg gaaggcgcag
     3301 tcgaacgtaa ccgcatgggc gagattatca ttgatgcgaa atgcgaaacc aacgtcaaag
     3361 gcgtgttcgc agcgggtgac tgtacgacgg ttccgtacaa gcagatcatc atcgccactg
     3421 gcgaaggtgc caaagcctct ctgagtgctt ttgactacct gattcgcacc aaaactgcat
     3481 aagaagaagt aagattcacc tgcaattgct tagccgccgg ggtcaaacct ggcggctttt
     3541 ttatggcatt aaaaagcccc gccggaatgc ctccgggcag ggctggagac ttattctggc
     3601 aattaacgca caaccagcac cggcagattg gcgtggcgga tcacgctcga ggcgttagaa
     3661 cctaacagat gggtcgaaat cgatgggttg cgagaaccaa taactacaac atcagccccc
     3721 agttcttctg ccaactcatt gacttcatcc cgcacgctac caaaacggac atgttgttta
     3781 atgcgggaag gatcgatggt gaagtggctg accatcgttt gcagacgttc ttctgcttca
     3841 tgttgcagat gctcttcaaa acgacgcaca tcagcggcaa aacggtgcag gctcaggctg
     3901 gctgacccgg gtagtacgtg aagtagatga ataactccgt catcctgggc gaggaattca
     3961 gcgtggcgaa cagctttgtc gctcaattcc atttcaaata catcaactgg cataatgatt
     4021 gtcttataca taaccctttc tccctgttaa tcatgaacaa atcattcgcc atgattataa
     4081 tatttatccc tgatatttgt ctggttcttt ttccttacga acggtttctg tgatcaatat
     4141 attctcactg aacaccagga attctcctaa aacctgcggt accgcccgtt ttcccgctgt
     4201 gatagctacc cttaaagact gactcttttt tgaactgtct ctggaggttg catgaaagca
     4261 ttgacttatc acggcccaca tcacgttcag gtagaaaatg ttcccgatcc gggcattgaa
     4321 caggcagatg atattattct gcgtattacg gcaacggcga tctgtggctc tgacctccat
     4381 ctttatcgag gcaaaatacc ccaggttaaa catggcgata tttttggtca tgaatttatg
     4441 ggggaagtcg ttgaaaccgg aaaggacgta aaaaatttgc aaaaaggcga ccgggtggta
     4501 attccgttcg tcattgcttg tggcgactgt tttttctgtc gattacagca atatgccgcc
     4561 tgcgaaaata ccaatgcggg taaaggcgct gcgctcaata aaaaacagat accagcaccc
     4621 gcggcattgt ttggttatag tcacctgtat ggcggcgttc ctggtgggca ggcggaatat
     4681 gtccgcgtcc ctaaagggaa tgtggggccg tttaaagtac cgcctttgct ttcagatgat
     4741 aaagcccttt tcctttctga tattctgcca acggcatggc aggcagcaaa aaatgcgcag
     4801 atccaacaag gttcaagcgt tgcagtctat ggtgctggtc ctgtgggatt gttgacaatc
     4861 gcctgtgcac ggttgctcgg tgcggaacag atttttgttg ttgatcatca tccctaccgc
     4921 ttgcatttcg ccgccgaccg ctacggcgcg atcccgatta attttgatga agacagcgat
     4981 ccggcacagt caattattga acaaacggca ggtcaccggg gcgtggatgc agtaatagac
     5041 gccgtcggtt ttgaagcgaa aggcagcacc acggaaacgg tgctgactaa cctgaaactg
     5101 gagggcagca gcggtaaagc gttgcgccag tgtattgcgg cggtcaggcg tggcggcatt
     5161 gttagcgtac cgggcgtcta cgctggattt attcacggtt tcctgtttgg cgacgccttt
     5221 gataaagggt tgacgtttaa aatgggacag acccacgttc acgcatggct gggagaatta
     5281 ttaccgttaa ttgagaaagg attactgaaa ccagaagaaa ttgttaccca ctatatgccg
     5341 tttgaagagg ccgcccgggg atatgagatt ttcgaaaaac gtgaagagga gtgccgtaag
     5401 gtgattctgg tacccggtgc acaaagcgca gaggcggcgc agaaggcggt ttcaggtctg
     5461 gttaatgcga tgccgggggg aacaatatga tcgtcaggag tggttttcga ggtaaaggac
     5521 agccatgacg ataatcgccg ccataattag aaatcctatc aggatgtaaa atgcttctgc
     5581 catggttatt cccacaaacg aaacggaata attttgcagc aaaagtgaac agtgagaacc
     5641 aggaaaaatt gctgattttg cgtaaagagg atgcgagtgc atcctctggg caaagcgagt
     5701 tatcgcttgt gcagatggga ttaaagcaga tagtcgccag cagcttctgg ctggtattcg
     5761 agttccagca cttcaaggtg ggttgcaacg ccgcccggaa gttcccagtg aatggaatcg
     5821 ccaacgcgca gccccagcag tgcggcacct accggagcca taacggaaag ctgagtattg
     5881 ctatcggtca tttttgccgg atacaccagc gtgcgcacac gcacttcgcc atcgctaaga
     5941 ttgcggaatt taacccggct gttcattgtc accacgtcgt gtggcatctc ttctggcgaa
     6001 cacatttggg cgcgatccaa ctctgcgttt aacgcgtcgg cgattggcaa accagcatag
     6061 gcgggttgct ccagcagaat atcgatgcgt tcggcatcca ggtcgttaat gatgatagtt
     6121 ggtctggaca tttttactcc atgtcgtcgg tactgcgagt gtcgcagata aacataccca
     6181 aaagaaaacc ctcaccgtca ggcggcgagg gtttaactca catgatgata ctgactgttg
     6241 ctcactcttt gaagtgattt gcgtcacatt cagggaattc ctcaatgcag cgcattatgt
     6301 ataattctta atcgtcatgg ttatggaaga cgaattagcg tgttttgtaa atcagaggat
     6361 taataacccg ctttatcaat cacaaaggtt ttgccacagt tacctgggtg aggttgtggc
     6421 aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga gatctgaatt
     6481 tcagtcaaat acgcagggtt accctggcac gttagcttaa ttgctttcac gttctctttt
     6541 ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt accgtaatta
     6601 tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg taccatcgta
     6661 ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt
     6721 tccaggcagg aacgtccgcc agctccaggc atgacttcac ttagtttagc ggccgtttcc
     6781 agtgataacc cggtttccgg cgatgaacac atccggctgg cgcgcgcttc tggtagattc
     6841 gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac accacgggca
     6901 gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc agctttgttg
     6961 gtcgtttcgg tttgcaggcg acattcatct cgttcgttac gatttcgatc gtgttgactc
     7021 tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc gccatactgt
     7081 tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac cgcgagcaac
     7141 gaggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac actgaatact
     7201 atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt gatgatttat
     7261 tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca accaacagca
     7321 acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct tttgatttca
     7381 catagccaca gccgtaaata atcacccctg gaccggttgc atacggcgtc agacagccca
     7441 tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg cccggaatac
     7501 ctttaccgac ggccagaata accggcagca tggttgcggt gtgcgcagac aggctggcaa
     7561 acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg tttggtgaga
     7621 atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaaacca gaacgagtca
     7681 ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat gcgctgttat
     7741 agcgggtaat gtctttccac ggcacaacgt gcagggccag cattagcgaa actgccagca
     7801 gaccaaccgc agtagcatta atgacttcac tgccaaatac ccacaaacct aagctgagca
     7861 atacaaggcc aatcagcgtc cactctctgc gtgtcagcgc acccatggtt tttagttcat
     7921 cacttgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac agcacgtagg
     7981 aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag aggaaccact
     8041 gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc agtacgtttg
     8101 gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc atcatccaca
     8161 tcaaatagcc gccaatacga cgtgcggacg gatcgttcgg gaatgattta aacagcggcg
     8221 gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa
     8281 acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc
     8341 ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact tcatacccta
     8401 atgcaaaaat aaatgcgcca aataccagcc atactgtggt gctggaaaaa ccagccagac
     8461 cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct ttggcatcaa
     8521 agagcaggta attactgcca ataacgcaaa tggtaaccgc aataaaactg atcgctgttg
     8581 ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg aagtaatgcc
     8641 atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacgccc atcaccacca
     8701 gtgg
//
LOCUS       DAZUGK010000185         8510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZUGK010000185 DAZUGK010000000
VERSION     DAZUGK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.186"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..2909)
                     /locus_tag="ACHY5W_003797"
     CDS             complement(39..2909)
                     /locus_tag="ACHY5W_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFS4448607.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AEREEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2906..3685)
                     /gene="ygfM"
                     /locus_tag="ACHY5W_003798"
     CDS             complement(2906..3685)
                     /gene="ygfM"
                     /locus_tag="ACHY5W_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFS4448608.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDRDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANDIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3736..5064)
                     /gene="ssnA"
                     /locus_tag="ACHY5W_003799"
     CDS             complement(3736..5064)
                     /gene="ssnA"
                     /locus_tag="ACHY5W_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFS4448609.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5067..8165)
                     /gene="ygfK"
                     /locus_tag="ACHY5W_003800"
     CDS             complement(5067..8165)
                     /gene="ygfK"
                     /locus_tag="ACHY5W_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFS4448610.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLSRHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTMLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINDKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCPPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         1967 a         2208 c         2223 g         2112 t
ORIGIN      
        1 aaacagagaa acaggccgcc gaagcggcca tttattactt atattttttc cagcgcggtg
       61 agtattttct ccggtgtgaa atgccattcg cgtaaccaga tgccgcaggc atcgtgaatt
      121 gcggtagcaa tcgccggagc tgcgccattt acaccaattt ccgagatcga tttcgccccg
      181 aacgggccga ctttatcgtc gctcggcacc aatacagcgc ggaaatcacg cggaatgtca
      241 ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca
      301 taaatgatct cttcgctcat actgtggccg atagcacgca gggtggcacc gtagatttgt
      361 cccaacgcta actctggatt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc
      421 aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg
      481 aaagcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccggtgccg
      541 gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg
      601 cccggcgttg ctagttgaac atctgccact ggctcaccca acatttgcgc gccgtggaac
      661 agaattttct cccgcagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta
      721 ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca
      781 tgcacgtcct gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc
      841 agaccagtac caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt
      901 ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg
      961 acgccgcgac cgatgtgcca gtcaccattt tgcggtttcg gtgaagacca ttggatcatc
     1021 tcgcgaccct gacgcaggat ttcttccagt gcgcagctgg cggcggaagg aacggaggtc
     1081 ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg
     1141 ttacgttcga taatttccag ttggtcgatc tgtaactgtt ctgccagttc cgctaatgcc
     1201 atggtgatag cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa
     1261 atgttgctgt agtaggtggt gacctggaaa tcgacgttat cgcacggata taagggcagc
     1321 gacagcgccg gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg
     1381 gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg
     1441 gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg
     1501 aacagtaccg gacgcccggt cacgcaggtt gcccatgcgc acacttcttc cagcaggatg
     1561 tcctgtttgg aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt
     1621 ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg
     1681 tggataacca gacgatcgcc atccatgcgg gtaaagcaga tatgtgtttc agtcgggcac
     1741 tgctgcgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct
     1801 ttgtccatat cgccaatatg accatgaata ctggcggcga tatttttgcg tggacgagaa
     1861 ccgatcggga agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct
     1921 tccagagtat ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca
     1981 tcttccgcca ttgcttcgtc gatcgacatt accggtttaa gcacttcata ttcaacgtcg
     2041 atgagcttca atgcttcaag cgcaatttct tctctttcag cgacgaccgc agcaacacga
     2101 tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt
     2161 gcgctctgac cacccggggt atagtagata tccgggcaat tcaggtgagt aataacgtga
     2221 acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg
     2281 tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag
     2341 cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgact
     2401 tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt
     2461 gcaaccgcta gttcaatgac ctgatagtat tgctgccagc cagcatcgcg gctgaacaaa
     2521 ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg
     2581 gtgataatca gagccgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc
     2641 attgcctgtt gaaccagact taactcgttc catttgccca gagattctgc ggtacgaata
     2701 tctgccttct ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag
     2761 attattgcgt cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt
     2821 cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc
     2881 gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg
     2941 catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca
     3001 aatcttcctg cgggaaaatg tcgttggcga cagcctgttc aagtgcattg ccttccagat
     3061 tttgtttttc gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc
     3121 gcataccgtc gtggtctgtt attgcaacgg ctgccgtcac gacggttaaa cctgcctgag
     3181 aacggctaat tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta
     3241 gcaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa
     3301 aaaccagttc agcatccagt gccagcagga cgggaagcag caccgactct tcctggcggg
     3361 cggcgatttc accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga
     3421 gggcttcacg cagcgctgca ggaataaatc gcgcatcacg cagtggctgc aagcgagaca
     3481 ttgcgccaat ccgcagtgca ccgttatccc tgtcaaccca gtccagttcc agatcctgta
     3541 aggagatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg
     3601 cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt
     3661 cgggcctgaa aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa
     3721 gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt
     3781 tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat
     3841 cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc
     3901 ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg ttaaatcagc
     3961 tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt
     4021 accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg
     4081 tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccat ctgttcccaa
     4141 tgccagattg cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga
     4201 acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga
     4261 caagtacagc ccatgagcga ccagcgtttt gctgtcgatg agatcgaatt gcgccagtcg
     4321 tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc
     4381 agcgtgaata tgcaaaccac ggcctgtggc tttcacggct tcacgcagca tctccagacc
     4441 ggcatctggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc
     4501 ggttgctgct ttcttcgcct catcaatctg acgggcgaag cggatgtttt cttctacacc
     4561 ttcctgtaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg
     4621 caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg
     4681 agaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat
     4741 cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt
     4801 tttcagcgtt gagatgaaat ccgggcaagg ggcgatgttt gccataattc cgcgggaaag
     4861 ccccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat
     4921 ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tctccgatag cgacaatcac
     4981 atcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc
     5041 agtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga
     5101 tgctggtgga catggctgat aatgcggcac atatcgttca gctccggtgg tacgttgtta
     5161 aactgacctt tgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc
     5221 cggcaatctt ccacaaggaa gcctgggttg ctgctgttat caaagtcttg cgccaggctg
     5281 aagacggtga ttttgtcttt gtacggttta ccgttccacg gacagaactg agcgcagttg
     5341 ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga aacggttctg gaagcctggg
     5401 accgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgct gcaaacgtag
     5461 ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc
     5521 accagcgtga tagagatatc gcctttacgt tgatagattt ccgccggatt gacgttgttc
     5581 cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtagcc
     5641 cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc
     5701 ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca gccgtttttg
     5761 tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg
     5821 atcaggctgt ctacaagcag tgttactgtt tcgttggttt caaccggacg acgacgacct
     5881 ttctcatccg gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa
     5941 cgttccggat tgttcaggaa acggaactcc acgccgtcgt gcaacgcttc ttcatactct
     6001 tcgcgccatg cgggcatctc ttgcagtgaa cgacggtaaa cgatcgttgc tttttctacg
     6061 cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg
     6121 accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt
     6181 gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt gtcggtgcct
     6241 gtggcaatca gaacataatg gaagccctga ttttttaact gttcaacggt taaatcgggt
     6301 gggcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt
     6361 aactctgcag gaatacggaa ctgagggatg atatttttca ccacgccgcc cgcattggct
     6421 tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga
     6481 cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc agctggtttg
     6541 tgccaacgtt gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg
     6601 atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa
     6661 atatgaccgg taatggcagg tagggcgttg cgttggtaga tgagttccag cgcgtcggca
     6721 tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca
     6781 gtaacacagg gggcaacgta gcagtcggtc agcggcaggt cttctgccac ttcaatacgc
     6841 tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc tgccagtctg
     6901 ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc
     6961 atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catcgtaatt
     7021 gggcgaatac cagtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa
     7081 gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcgacatt gatggagagc
     7141 gggaacagcg cacggcctga catatacatc tcttcaccgg gcagtgcgcc tttatcgttg
     7201 atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt
     7261 gccatcaggc gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac
     7321 tcttctttta agcctatgta accgaaaccg caaacatcga ggatctcacg aacacgcgcg
     7381 tacccgagca tggtcgggtt aagtttgaca aaggtgttga gccctttttc ttccagcatg
     7441 tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg
     7501 ccctgcacca tactggtagg aatgcgagcg ggtaaagctt gcaagctttc gcgtttttcc
     7561 tgcaatccgt ggcgggataa aaatgcgtca tcctggagta atttattcag cgtatcgcga
     7621 tattgcgcga atttcggatg gtcagatgca tccatcatat tgtcgatgaa ctgttgcatc
     7681 ggcggttgct taataccttc gaggttataa ccgacgctca tattaaagat gaacgattta
     7741 ccggaatcag aaggctggaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg
     7801 tattcatccc aggctttaag cagggtaaat tcggtagacc attcggtgtt aaagcactcg
     7861 tcttcggcat cgatacaagg cttttccagc tccaggcggt caagaatttg gacggttttt
     7921 agttcgatga agcgtccgcc agtcagccag gaggtgacaa tattttgcgc gagctgcgtg
     7981 tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt
     8041 ttacctttta cgggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt
     8101 tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc
     8161 cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg
     8221 gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata
     8281 aaaaaatgtg aggcggctcg aaacttaagg gctttttgtg aatgaactca caggtgccaa
     8341 atgagaatta aaatcactcc tgaaaattat tattgccagc ccatttatca ctttttctct
     8401 tttctcaaaa ttagtgattc cttatcatta tgataaagag tgctggaggg gttaaatatt
     8461 tgaacgcaat cgtttaacta cgttttctaa atttcagtat accttttctt
//
LOCUS       DAZUGK010000186         8454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZUGK010000186 DAZUGK010000000
VERSION     DAZUGK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.187"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..378)
                     /locus_tag="ACHY5W_003801"
     CDS             complement(112..378)
                     /locus_tag="ACHY5W_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFS4448611.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(375..1142)
                     /gene="yjiL"
                     /locus_tag="ACHY5W_003802"
     CDS             complement(375..1142)
                     /gene="yjiL"
                     /locus_tag="ACHY5W_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFS4448612.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFHPATAITEAW
                     ETLREGLETSPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCESPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1152..2303)
                     /locus_tag="ACHY5W_003803"
     CDS             complement(1152..2303)
                     /locus_tag="ACHY5W_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFS4448613.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2419..3699)
                     /locus_tag="ACHY5W_003804"
     CDS             complement(2419..3699)
                     /locus_tag="ACHY5W_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFS4448614.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLTELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3740..4972)
                     /gene="mdtM"
                     /locus_tag="ACHY5W_003805"
     CDS             complement(3740..4972)
                     /gene="mdtM"
                     /locus_tag="ACHY5W_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFS4448615.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAVVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5439..6383
                     /locus_tag="ACHY5W_003806"
                     /pseudo
     CDS             5439..6383
                     /locus_tag="ACHY5W_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(6625..8037)
                     /locus_tag="ACHY5W_003807"
     CDS             complement(6625..8037)
                     /locus_tag="ACHY5W_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4448616.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSMALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8214..8378
                     /locus_tag="ACHY5W_003808"
     CDS             8214..8378
                     /locus_tag="ACHY5W_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFS4448617.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
BASE COUNT         2185 a         2256 c         2149 g         1864 t
ORIGIN      
        1 atcgctcatt ttgtggatgg gaaatatttc atggcaggtg agatatttaa tacaacttcc
       61 ttcatgcagg attatgcgca gccaattatc cgcgcgttat atttaaagat cttattgcct
      121 ggttgacgct tctgcatcgt aatgcaccac gcactgccag tcgctgccct gctggcaata
      181 tatcgcttcg gtttgcccag ggatcaccca ttgtatttcc tcgccggggg gacaggtcag
      241 ccaaatgcat aacccgcagc cgccgcgtaa atcacgggga atatcgctga cgcgaaaagt
      301 catgcccgct gcctgcaacg ccttgcgggt ttgtatgacg ccgaccgtgg agtgaaataa
      361 aaataaaaac tctttcatcg gcgtgtcctc actcgttgac caattaccgc cgcgccaatt
      421 gcgccagcaa attgcgcatc aggatgggta tttaccggca ttcccaggtg agattccagc
      481 atcctggcaa acgtctggca atgactaacg ccaccagtaa acagaatcgg cgattcacag
      541 gagagacgag caatgaaatt ggcactccgc cgcgccatcg cgttaatcac tccggcgaga
      601 atcgcttctg gcgcgacgcc cgctgagcgc aggctgatca cttctgattc agcaaacact
      661 gtgcacatac tcgtgatggc gtgcggcgtg acattttcgg taatgctgtc gagttgctcg
      721 acgctggtgc caagcgtgcg cgagatcacc tccaggaaac gcccggtgcc cgccgcgcat
      781 ttgtcattca tcaggaaatc gcacaggtta ccgtcatcat caagctgaat cactttgctg
      841 tcctgaccac cgatgtcgat taccgcgcgc gttgctggcg caagaaaccg tgcgcccagc
      901 ccatgacagg agatttccgt cacctgttta tcggcaaaat ccaccagttg ccgcccgtag
      961 ccggtgagcg tcagaaacgg cgatgtctct aacccttcgc gcagagtttc ccaggcttca
     1021 gtaattgctg ttgccggatg aaagggtgtt ggaacgagga aacggcgcgt aatcacgccg
     1081 tctgccagta agatcccttt ggtggcggta gaaccagaat caatgccaat cgaatatgtc
     1141 actgccactc cttacagcat ctcaataaag gccgcgacac gggtactgag ctgcccgaca
     1201 tctgaggtgg agtagtctgt ttcaatagcg atataaggaa tgttgtgctg ctgacgcaca
     1261 tgacgtttaa tcgccagcga ttccaccgcg taggtatggc acgcctgtaa aatcacatca
     1321 actacgccat cgacctgata ttcttccacc atctggctga gcattttcag gcgctgatcg
     1381 ttcggcgaaa cacaggagca gccaatcgcc agatatttat ccgccagcgc gtcgtagaca
     1441 tcgcccgttt ctgccacgca ttgctcggtc gctttcgccc cggtgcagtt ttcataaccg
     1501 acaacccagc cgccattctc ttcaatcgcg cgcaccactt tctctgctgc gccgccaatc
     1561 gggcagccgg tgattaaaat gcgcggacgc gggtccagtc gctggccttc ttcccactgc
     1621 tgacgaatgc gggcggtcat cgcgtccagt tcattgatca acgcctcttt atcgaaccgg
     1681 aaggttgcac cgtaaaccac tttcagaatg tcgctgccgc taagcgccgg aggattgaac
     1741 tgcccaagat gataaaaatt ggccagtgcg cgacgttcgc ggtttttcag cgcaatggca
     1801 tcgcgcagag catcttcgct aatctcgtgc ccaaaacgtt cttccaccgc tttttgtaag
     1861 cgcagcatct cggctttcca taacgcacgc gaggcatcgt ccttaacgct gttcggcaac
     1921 tgcatcacat gaacaggctt aaactccgcc atgtattcat acattttctt tttgccgtcg
     1981 caggtggttt caccgaccac cagatccgaa aagtagaagt aggggcattt atcggttttg
     2041 ccaaagccgt agctgctttt aatcagcggg cagaggttgc gcggcagatc tttctccgct
     2101 tcttcaatgg tttcatcaga ggtggaacag agcgaaacca caaccgcacc ggctgccatc
     2161 gggatctctt gcggcataaa ggtgcagtaa gtgccaacca gcggaatgcc gcgctccttg
     2221 agatccatga cggtgagaaa gcctttctgg cgagcttcag agaactgatc gaaaatggcg
     2281 ggtagatcgg tgacaagtga catgattttc cttccccgta ccacgggaga taatgaaaag
     2341 aggccacatt attaccattc ttttagtatg gtttatttga tctctctcgc ggttagccac
     2401 ttagtttttc atggatttct aaaaattgcc gagggggaac aaggccggga gctgcgacag
     2461 caaatataaa attagcccaa tacaaccgcc aaccagcgta ccgttgacac gaataaactg
     2521 cagatctttg ccgatattta actctatttg ccgcgacata tcccgggcat cccagctttt
     2581 taccgtatcg ctgatgtggc gcgtcaggaa tgcggaaaac tcaggcgcga cgcggtgcgc
     2641 ggcttgttcc agatgaccat ttaacgacgc ccgcaaggca tcatcggcaa ttaacgtttc
     2701 gccaaaccat tgacccgccc gcgcgatacg ttctttcaca cgagaatctt cactgttgat
     2761 atccgctttc agccattccc gtaaatcccc ccacaattca gtgagatagc ggttaaaagc
     2821 ttcatcttct ttcagataac tttttacggc atcggctcgc gctgccattt ccggatcgtt
     2881 tttcagcttg tcgatcaggg caaaagtggc gcgatcaaac gcatggcgga tctgatgcgc
     2941 gcgatcgcgg ctgatatcat caagcaaaga attcaccgcg tcagaaacca actccgcgct
     3001 atgttcgccc agccattcgg tgggcaaaat tttggctttc agcggatgct cgctctccag
     3061 ccagcgaaca atttgctggg cgataaactt gcgcgattta tcgcgctgga gaagggcgat
     3121 caactgtgcg atcagcgtat cgagcagcac ctgatgacga tcgtttttgg tcatactctc
     3181 cagcatcaac gcactggttc cggaaagatc gaccttatca atcgcccgat ggaccgcgcg
     3241 cttaagcagg cgctgaatac gcgcatcatc ggtcagttca agaaaaccgc tcataatctg
     3301 caacagatgc tgaccaacgc ggcgggcgtt ttctggctga ctaaaccagt tgccaatcaa
     3361 caacgccggt tcgtgacgtc gaatcaatgc caccagcgac tgggtatcga gaaatttttc
     3421 ctgcacgaac tgaccgagat tttctccaat ccggtcttta ttacgcggga taatcgccgt
     3481 atgacgagaa atgatcggaa ttggcacgcg gcgaaacagc gccaccaccg caaaccagtc
     3541 cgccagcgcg ccgaccatcg ccgcttcagc aatcgccttc acgccgctca cccaaaaatt
     3601 gggcggcaaa aacagcgtaa cgacaaaggt agcagcggcg ataagcagta aagagagcgc
     3661 caacattttg gcgcgtctga gttcaatgag tttattcata acgttaagaa tagagcctga
     3721 cggcaggatc gcgcaaaaat caccgctcct ccgccagctc ggcagcctga tgttggcgca
     3781 cgcgattaag caatccggcc agggtgaaaa cgacgataac gcccgccaca acggctaaca
     3841 gatgaaacgg caagcgaccg ccgttaaacc acagccagcg gccaatttcg accgagacag
     3901 acatcaccat caggatcacc atattcagcg atgcggagac ggtccctttc ggtaagttat
     3961 tggaaaacag cgtaaagcgg aataaggtcg ggaagatcaa accaatcccg aaagcataca
     4021 gactggtgcc cagcaccgac cacagccaga cgtgcggtga cagcagattg ccgataatca
     4081 acagcgcgag gccgaccagt tgaatgggta cggcacgcca gataaagcgc ggttcggtcg
     4141 gatctttaac gaaacgcgcc acgacggcat tcgcgacaat caccgcgccg aacaccggaa
     4201 cctgtgtcca ggcgaactgc gaagttgtta agccgcccgc gtcgatcagg atcaccggcg
     4261 agacagccac ccaactcatc attgggatat agcttaaaga gatggttgct gcgccaaaga
     4321 ggaacagccg attgcaaaag acattacgaa aatcgcgtaa tacgcttttg gcgctaaacg
     4381 gaaccgcgcc gcgcttcacc gtctctggca tcgccagcag taagccaaca aatgagataa
     4441 aacccataac cgcaatgatg gcaaaaagga ctttccagtg cacaaagtgc atcagagctg
     4501 cgccggaaag cgggccaata atcggcgcaa tcagtacgat ggaggtgata atcgccatca
     4561 acttgatccc ttttgtctgt ccgaacgcct cctgcaccgt gacataacca acggtagcaa
     4621 taaaacagat actggtgccc tgaattgcac gcgcgataag aaactgtgtc atagacgttg
     4681 tgaacattgt cgcagcgcag gcaagggtaa aaatgagcgc cccggtaatc agcactggcc
     4741 tgcggccaat cctgtcggaa agtggcccca gcagccactg taacgccata ccgccagcga
     4801 gatagagact gacggcagca ggggccagac tgacatcggc attaaaatca cggaccacat
     4861 taatgatccc cggctggatc agatccgtcg aaagataggc ggcaaagtca tacaaaatca
     4921 acgccatcgg gaaaaacagc gtagcggcat ggcgggcaaa aaaacgtggc ataacaaaca
     4981 actccttgtc cggagaaccc caacggggaa aacgaaattc aataccgacg gtaatgacga
     5041 aaggcaggcg agaaggacaa atgtactgaa tttcatgtaa tcaaccacac tgcctgtgaa
     5101 cacacagaca gagaaatacc ttctgactcc aggtcagaat gtataacgat tacaaatagt
     5161 catcagcaca ttacacctct ttaattgatg ggttgcatag aaaatataca ccttaagtgt
     5221 aattaaaatt tgcggttaat caaaagagcg cggtggaaag gggaatatct gccgggtaca
     5281 ggaaatgtaa aacgcgagcc tgtttccgga aaaatgtatc gtttgcagag gaacaaaaat
     5341 agcctggaaa gcgcctcggg gaacggaaaa tagccaggtg agaatggttt tgttagtcgc
     5401 taaagtcggg ccatcttatc tacaggtgac ggatcgccat gacaaacttc acgaccagca
     5461 cgccgcatga cgcattattt aagtcctttc tcacgcaccc tgacaccgcg cgggatttta
     5521 tggagatcca cttacccaaa gatttacgtg aactgtgaga tctcgacagc ttaaaactgg
     5581 aatccgccag ctttgtcgat gaaaaattgc gggcgctaca ctccgatatt ctgtggtcgg
     5641 tagagacccg tgaaggtgat ggctatatct atgtggtgat tgaacatcag agccgtgagg
     5701 atattcatat agcctttcgc ctgatgcgct attccatggc ggtgatgcag cgccatatcg
     5761 agcatgataa acgccggccg ttaccgctgg tcatcccgat gctgttttat cacggtagcc
     5821 gtagtcctta tccctggtcc ctgtgctggc tggacgcatt tgccgacccg actactgcac
     5881 ggaaacttta tactgcagcg ttcccgctgg tggatgtcac tgtcgtgcca gacgacgaga
     5941 ttgtgcagca ccgcagagtc gccctgttgg agttgatcca aaagcatatt cgccagcgcg
     6001 atctaatggg gcttattgat caactggcag tattactggt tacagggtgc gctaatgaca
     6061 gccagataac cgcgctgtta aattacattt tactggctgg cgatgaagcg agttttaaag
     6121 agtttatcag cgaactaacc agtcgaatgc cacaacacag ggagcgaata atgacgattg
     6181 cagagcgaat tcataatgat ggatggctgt tagggatggt aaaggggaaa gaagaagggg
     6241 aacaacgcct ccttagattg cttttgcaga acggtgcgga tcctgaatgg atacaacgat
     6301 atactggact ttcggcagag caaatgcagg cattagagca acccttgcct gaaagcaagc
     6361 gcgatccatg gatcgagtac taatcagaga ctgatgacaa acgcaaagct gcctgatgtg
     6421 ctacgcttat cagacctaca ttacctctgc aatctattga atttgcgcga tttgtaagcc
     6481 ggataaggcg ttcacgccgc atccggtatg aacaaagcac actttgccta agccatgctg
     6541 gaacgtggcg atgcccctgc aaagataaaa cgagtcacag gcgattcgtg agaggaaatc
     6601 aggggcctcg ccactaattc gaattcattc cattgcctga tacacggcct cgccaatttg
     6661 cttcagtgct tcgcgatagg tttcgctgag cggcaaagcg cagttgatgc gcaggcagtt
     6721 acggtatttg ccggaagctg agaaaatcga gcctgccgcc acctggattt tcatgcggca
     6781 cagctgccgc gcgacgcaga ccatatcgac ctgttcaggc aattctatcc acagtaaaaa
     6841 tccgcctttc gggcgcgtaa tacagatttc gcagggaaaa tattcccgta tccagcaggt
     6901 ataaagcgcc agattgcgct gataagtctg ccgcatccgc cggatatggc gatgatagtg
     6961 accttccaga acaaacgttg ccgccgccat ttgcgtggac ggcacattaa agctgctgat
     7021 ggcgtatttc atatgcagca gtttatcgtg ataacgcccc ggtgcgaccc aacctacgcg
     7081 caggcctgga gcaatacttt tactgaacga gctgcacaac agcactcgcc cgtcgatatc
     7141 ccaggaatga atggtccgcg ggcgcggata ctccgttgcc agttcgccgt agacatcatc
     7201 ttcaaaaatc acaatatcat gacgctgagc gagagagaga acggcccgtt tgcgcgcgtc
     7261 cggcataata aatcccagcg gattattaca gtttggcacc agaatgatgc ctttaatcgg
     7321 ccactgttcc agcgccagtt ccagcgcttc aacgctgatg cctgtttctg gatcggttgg
     7381 gatttcaatc actttcacgc ccatgccgcg cagcatctgc attgaaccgt agtaacaggg
     7441 ggattcgacc gcgacaatat cgcccggttt acacaccgcc attaacgcca tcgacatcga
     7501 gttatggcag ccgctggtga tgatgatgtc atcggcggtg accaccgagc cgctgtcgag
     7561 catcaggcgg gcaatttgct ctcgcaacac tcgctgaccg gctaacaagt cataaccgag
     7621 aacggtttgc agattatgct gtaccacccg gcttagctcc cgccagagtg gtttcaggct
     7681 gggcgtttcg acatccggcg tgcttttgct taatggaaca atggaactgt cgctatgcgc
     7741 ctccagcata tccagtacct gatcccactg ggtaatttcc accgggcgct gcaccggacg
     7801 cgtcatcggc ggcactggcg gctgggcttt acgttgtgcg acaaaatacc ccgaacgcgg
     7861 ctgcggagtg atgagcttca tcgtctccag cgtctgatac gcctgctgca cggtgctgat
     7921 gctgacgccg tgctcctgac ttaagctgcg caccgacggc aatttctccc cgtgacgata
     7981 cagcccttgc tcaatccgct cggcaagcag agttgccaga tgttgataac gcgtcatgct
     8041 gtattcctta tttggaccat acagagagaa aaaccggtac agatgaggct aaaaaaccgg
     8101 atgcagatgc tgttttaacc tgtctgtatg ttaaataaaa ctaatatttg catctgtatt
     8161 gtgaactgcc agaagacgga tgatgaagcc tctgaaagat gaggaggcag cagatggaat
     8221 ttcacgaaaa caaagccaaa acgccgttta tcagacttgt gcaactctgg caggcggtga
     8281 ggcgttggcg gcggcaaatg cagacccgac gagtgttaca gcagatgagt gatgagcggt
     8341 tgagggatat cgggttacgc agggaggatg tggagtgaac tatgggcggc gtgttggcaa
     8401 atgctgcctg tctgcattca aaattgctac aaaatccaac cataaaagtc gttt
//
LOCUS       DAZUGK010000187         8412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZUGK010000187 DAZUGK010000000
VERSION     DAZUGK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.188"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..2476
                     /gene="ppsA"
                     /locus_tag="ACHY5W_003809"
     CDS             98..2476
                     /gene="ppsA"
                     /locus_tag="ACHY5W_003809"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFS4448618.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHY5W_003810"
     CDS             complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHY5W_003810"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFS4448619.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVVLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPLLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLVEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4236..4529)
                     /locus_tag="ACHY5W_003811"
     CDS             complement(4236..4529)
                     /locus_tag="ACHY5W_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS4448620.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4526..5815)
                     /locus_tag="ACHY5W_003812"
     CDS             complement(4526..5815)
                     /locus_tag="ACHY5W_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4448621.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLIEQAEQAGTQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5871..6809)
                     /locus_tag="ACHY5W_003813"
     CDS             complement(5871..6809)
                     /locus_tag="ACHY5W_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFS4448622.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6829..7593)
                     /locus_tag="ACHY5W_003814"
                     /pseudo
     CDS             complement(6829..7593)
                     /locus_tag="ACHY5W_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            7909..>8412
                     /locus_tag="ACHY5W_003815"
     CDS             7909..>8412
                     /locus_tag="ACHY5W_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4448623.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWEAFAYTLAIL
                     YYENFKNA"
BASE COUNT         2016 a         2176 c         2178 g         2042 t
ORIGIN      
        1 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
       61 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
      121 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
      181 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggggtttccg ttccgaatgg
      241 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
      301 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
      361 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
      421 ccgagaagcc tatgcacagc tttccgctga tgacgaaaat gcctcttttg cggtgcgctc
      481 ctccgccacc gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct
      541 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
      601 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gtgtggcgct
      661 ctccgccggt gttcaacgga tggtgcgctc tgacctcgca tcatctggcg tgatgttctc
      721 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acatccgcat ggggccttgg
      781 tgagatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcata aaccgacact
      841 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
      901 ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
      961 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     1021 aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg gccacaccgg
     1081 taaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     1141 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     1201 catcggtgcg ggtccggtga aagtcatcca tgatatcagc gaaatgaacc gcatcgaacc
     1261 tggtgacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     1321 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     1381 actgggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggtga
     1441 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     1501 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
     1561 gaacgtcggt aacccggacc gagctttcga cttcgcctgt ctgccgaacg aaggcgtggg
     1621 acttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     1681 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     1741 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     1801 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     1861 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     1921 tggcgcgggc cgctatgttt ccgacagctt ccgcgactgt ttcgcgctgg agtgtgaagc
     1981 agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     2041 gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg
     2101 tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
     2161 cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata tgacgcagct
     2221 ggcgctcggt ctggaccgtg actccggcgt ggtgtctgaa ttgttcgatg agcgcaacga
     2281 tgcggtgaaa gcactgctgt cgatggctat ccgtgccgcg aagaaacagg gcaaatatgt
     2341 cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt tgatggaaga
     2401 ggggatcgat agcctgtctc tgaatccgga caccgtggtg caaacctggt taagcctggc
     2461 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccggggtt atgtgatctc
     2521 cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc gcataatatc
     2581 ttttcttaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt tttcgattac
     2641 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
     2701 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     2761 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     2821 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     2881 tttgcgtccg gtaattttta tatagccagc ctcatccatg cggcagagat cgccgctgta
     2941 ataccagcct tcttcatcca gggcacgggc ggttaattca ggttcatcaa aataccccat
     3001 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aggtcttgcg
     3061 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgcataaa
     3121 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     3181 aacacttaat aatttaatgc cgcgctgctg gcattcacgc gccacttttt tggggattgt
     3241 ggtaccgccg caaagaaaga agcgcagcgc tgaaaggtct gcgggttgtt tctctactaa
     3301 attcaaaaga tcatagacaa acggcgttgc gccgagcata caggtgcaac gctgctgctc
     3361 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcaccaat
     3421 caataatggt gctgttacgc catgcagaaa gcccgttgcg tggccgagtg gcgcaggcat
     3481 cataaagaca tcctgccaag tcagattcag ccgcgcgcaa taagcccgct cactggcgag
     3541 aatattgtta tgcgtaagca ccacgccctt tggcagaccc tcggttccgg aggtaaacag
     3601 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc
     3661 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
     3721 ttgttgaagt tgtggaagct gattttgcag cggcaggatt aaatctaccg gacgcgtttg
     3781 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccataccag
     3841 ttctgcttcc cgccaggaag gcaacagcgg tacggaaacc gcaccgattt tcaggcaggc
     3901 aagatagata acggtaaatt cacaccagcc tggcagttga aatgcgatgc gatcgcctga
     3961 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     4021 ataggtgtac gatgctccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
     4081 ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct gacgatacgc
     4141 cgcacgacgt tgttcgttaa acgttaatgt cactttcatt ttggactctc gaaacagaac
     4201 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
     4261 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
     4321 gtgccgcact ccagacaacc ggcagaatca aaatgaatgt tgccggcgtc atcctgctta
     4381 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
     4441 gccaaaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
     4501 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     4561 gcaagttgat cagcccaatt ttcttcgcat gtcccatgat catttttcgt accggctggt
     4621 ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggcgaccatg cgcggatact
     4681 gactaaacag gcgcgggttt tccatcagcg ccgggatctt gcgaaaatgc tgcatatcgc
     4741 gcataacaca actttgttcc agctcacgtt tgtattgcgc aagactgctg gcggagaaat
     4801 ccgcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat gcaatggcta
     4861 aatccatgcc gcggaccgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     4921 tcatcacgcc ctcgttaacc agctgcggca ccattgccag accgccttcc ggcaccatat
     4981 gcgcggaata ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg gggtgttgtt
     5041 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac
     5101 aaaccagccc caacgatatg gaatccttat tggtatagag gaatccgcca cccatcaggc
     5161 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
     5221 gatcgttgat ctgttctggt gtcaggccaa taacctcttt aacaccaaca gcgtaatgat
     5281 gcggatcgga agcgggaacc attcccagcg aacggccaag catcgagtta acgccatcag
     5341 ccagaatcac cacattcgct tcgagaatat catccccagc ctggacgcca gtgaccttgt
     5401 ttccttcacg aaccagcgca tcgacgcgaa ctcccgggat aaactgcgtg cccgcctgct
     5461 cggcttgttc aatcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
     5521 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
     5581 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca aagcctggaa
     5641 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
     5701 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa
     5761 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt
     5821 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
     5881 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
     5941 tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tgcgccgttg
     6001 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
     6061 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
     6121 attggacgtg aacagccgac ttcagcattc agaaccgccg ccagttcgtg gaccattttc
     6181 aggtcatcct gcgctgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
     6241 tccacgctgc ttgccgcttt cgcacggcgc tcgcgacaga gaatttcatg acgcggagca
     6301 acgtaaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
     6361 acaccgggtg caagggtaat gatcgccagc gagctgttga tcttttcctg agcgaacgct
     6421 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
     6481 gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
     6541 cgtttcgtcg cggccagtag caacatagcg gggtgtttat ctttcagcag agcagcaatg
     6601 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
     6661 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
     6721 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
     6781 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
     6841 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgagaatgat
     6901 gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc agttcagcaa gtggtgcgct
     6961 ctggctccag ccaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
     7021 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
     7081 tggaacagag agttcaataa cttcaacgtc atcttcaagc gtgcgttcaa tcaccagtgt
     7141 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
     7201 gaccagcaag ccaacctact gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
     7261 atcgaagccg atcttttcaa ctgctgccgc cagcgctttt gcggtatcga gaggcagtgc
     7321 atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg ataacacgtc
     7381 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
     7441 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
     7501 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
     7561 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
     7621 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
     7681 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
     7741 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
     7801 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
     7861 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
     7921 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
     7981 ttcgcattta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
     8041 gagctgattt acgtcaagaa aggggtggca aggtttacca tcgattcttc gttatatgtc
     8101 gcacatgccg atgacattgt ggtgatagaa cgtggcaggc tgcatgcggt ggcttctgac
     8161 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
     8221 gaggaaaatc agctactgca accgcattct tgcccggtaa ttgccgcagg gcagggaaaa
     8281 gaagtcatta aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
     8341 caaacatctt cgttatggga ggcatttgcc tatacgttag caattcttta ctacgaaaac
     8401 tttaaaaatg ct
//
LOCUS       DAZUGK010000188         8403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZUGK010000188 DAZUGK010000000
VERSION     DAZUGK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.189"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(279..1169)
                     /gene="malG"
                     /locus_tag="ACHY5W_003816"
     CDS             complement(279..1169)
                     /gene="malG"
                     /locus_tag="ACHY5W_003816"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFS4448624.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1184..2728)
                     /gene="malF"
                     /locus_tag="ACHY5W_003817"
     CDS             complement(1184..2728)
                     /gene="malF"
                     /locus_tag="ACHY5W_003817"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFS4448625.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYIYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETPAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2882..4072)
                     /gene="malE"
                     /locus_tag="ACHY5W_003818"
     CDS             complement(2882..4072)
                     /gene="malE"
                     /locus_tag="ACHY5W_003818"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFS4448626.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4437..5552
                     /gene="malK"
                     /locus_tag="ACHY5W_003819"
     CDS             4437..5552
                     /gene="malK"
                     /locus_tag="ACHY5W_003819"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFS4448627.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     ELVEEGATFAIGLPPERCHLFREDGTACRRLHQEPGV"
     gene            5624..6964
                     /gene="lamB"
                     /locus_tag="ACHY5W_003820"
     CDS             5624..6964
                     /gene="lamB"
                     /locus_tag="ACHY5W_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFS4448628.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADTNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7207..8127
                     /gene="malM"
                     /locus_tag="ACHY5W_003821"
     CDS             7207..8127
                     /gene="malM"
                     /locus_tag="ACHY5W_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFS4448629.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLAEVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2078 a         2191 c         2167 g         1967 t
ORIGIN      
        1 ttacgtaatg tcttttctgt gatcttaatt gtgataatta tcaaaaattg atgaaaacaa
       61 tactattgcc gtgactcaga gcacgaaaga gaattatcgt aagtgggaaa acaaataacg
      121 taaaaataat aagctctatg atggaaatat taaccggcga acgattcaga ttgcagacga
      181 aagaaaaaaa ggcgctccgt ggagcgccga ataacagtca caagttggga taacgtaagt
      241 tgagggtgca gcggcataac attggcagaa caacatcttt aacctttcac accacctgcc
      301 gtcaggccgt tgaccaacca gcgttgagcc agcaagaaga cgatggtgat cggtaatgca
      361 gacatcacgg cagcggcggc aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc
      421 tgcatcccca cggccagggt gtagctgttt acgtcacgca gtaacagcga cgcgaccgga
      481 acttcagtaa tggcagcgat aaacgacagg atgaacacca ccgccagaat cggtactgac
      541 agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg caccatccag cgccgccgct
      601 tcttccagcg aactgtcgat ggtttcgaaa tagcctttga tggtccagac atgcagcgca
      661 atcccaccca gatacgcgaa aattacaccg ccgtgagtat tcaggccaat gaatggaatg
      721 tactcaccca gacgatcaaa caacgcatac aacgcgacca gtgaaagtac tgccgggaac
      781 atctggaaaa tcagcattcc tttcagcagc gtcgctttgc ctggaaagcg catacgggcg
      841 aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc
      901 tttacagagt tccataacca caacaggacc gggaatggcg gtggcgtaat gcgaccatca
      961 gcctgttcca cgctaaaacc taacgccagt ttccagtgat cccaggagat ttgctccggg
     1021 atcaggctac cggtcgcaaa gttcccttgg cgcagtgaga tggcgacgac catcagcagc
     1081 ggaaacataa tcgccgcgat aaaaagtagc agtagcaggt gagtaataaa caaacgtgct
     1141 ttttgcgatt tcggttggac cattgccatt tttgttatct cccttaatca aacttcattc
     1201 gcgtggcttt caggttcact atcgccagcg cacccaccag caggaagatc agcgtggcta
     1261 ttgctgctgc cagaccgaag tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt
     1321 agttaacaag caggtcggta taaccggctg gcgtggtcgt gccaagacga tccgggccgc
     1381 cgttggttaa cagttgaatc agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca
     1441 tcagcggcgt cagcggttta atcagcagcg gcagcgtaat cttaaagaag ttctggaacg
     1501 gacctgcacc atccattgct gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc
     1561 ccatgcagag gatcatcatg tacggatagc ccagccaggt attgacgata atcagcatcg
     1621 ttcgggcggt ggtcggatcg ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca
     1681 tcatgttgat ctcaccgaag ctctggttaa acaacccttt gaaaatcaag attgaaatga
     1741 acgatggcac cgcgtagggc agaatcagca ggacgcgata gaccgctttg ccacgcaacg
     1801 cttcccactg caccagacac gccagaacca tgccgaccgc caccgttaaa aagacagtga
     1861 tcagtgaaaa caccacggtc cagacgaaaa tggcgaggaa cggtttctga atgccttcgt
     1921 cggtgaagac gcgggtaaag tttttccagc cggtggtcac ggtgtaaccg gggcttagct
     1981 tttcatcacc ccagttgccg tcggcggtaa tggactggta aaagccaatt tggttattcg
     2041 gacgatattt cacgccgctc tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga
     2101 gcggctgcgt gccagaaaac tggcgcaggg agctcatcat cactttgttg ccatccggca
     2161 gaatggcggt aatgtcactc agcgcctgac gattctgggt aatcacgcgc agattcgcgc
     2221 gttcgccttc gggctgggcg ggcgtttctt tcagttgcag tttttgctcg ccgccaaatt
     2281 taaaagcgtc ggagaggtaa tttttgccgg tttcgccgtc gctgagcgcc agttgccact
     2341 catcgcccgc cgggtaaaga ccaaagttat aggttttgcc tgcttgccag gagcgatcta
     2401 acaacacttc ctgtgcacgt tcaaaagtca gctggttagt gctgctgtag ttggtgaagg
     2461 caatggcgat ggtgcagacc agagggaaga ggacgaataa tcccattcca gccattcccg
     2521 ggtaaatata gcgccaggcg taggctttac gattggcgaa aatatacagc cccgctgaac
     2581 tcaatatcag cgtggtaatg gcgaacaggt attccccttg tgcgtacatt aaaacaacaa
     2641 ggtaacccac cagcaggccg agcagaccta gcactgacca tttcagcgcg tcgctttgcc
     2701 accaatgttt ctttttaatg acatccatgg ggttcttcct cattccagga cggataaggc
     2761 tttcacgcct tatccgacaa caactgcctg atgcgacgct gacgcgtctt atcaggccta
     2821 catacgtttc ggttttgtag gccggacaag gcgttcacgc cacatccggc atttcacagc
     2881 attacttggt gatacgagtc tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc
     2941 tggcggcgtt gatcaccgca gtacgcacgg cataccagaa agcggacatc tgcgggatgt
     3001 tcggcatgat ttcacctttc tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg
     3061 ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct ttattaaccg
     3121 cttccagacc ttcgtcagtc agcagatagt tttcgaggaa ctctttcgcc agttctttgt
     3181 tcggactggc ggcgttaata ccggcgctca gcacgccaac gaacggttta gatggttgac
     3241 ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg atgttggacc
     3301 atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca gcttctgcga
     3361 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca
     3421 gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt
     3481 catacttgaa cgcataaccc ccgtcagcag caatcagcgg ccaggtgaag tacggttctt
     3541 gcaggttgaa catcagcgcg ctcttacctt tcgctttcag ttctttatcc agcgccggga
     3601 tctcttccca ggtttttggt gggttcggca gcagatcttt gttataaatc agcgataacg
     3661 cttcaacagc gatcgggtaa gcaatcagct tgccgttgta acgtacggca tcccaggtaa
     3721 acggatacag cttgtcctgg aacgctttgt ccggggtgat ttcagccaac aggccagatt
     3781 gagcgtagcc accaaaacgg tcgtgcgccc agaagataat gtcagggcca tcgccagttg
     3841 ccgcaacctg agggaatttc tcttccagtt tatccggatg ctcaacggtg actttaattc
     3901 cggtatcttt ctcgaatttc ttaccgactt cagcgagacc gttatagcct ttatcgccgt
     3961 taatccagat taccagttta ccttcttcga ttttggcgag agccgaggcg gaaaacatca
     4021 tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt ttttattttc ataatctatg
     4081 gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga ctctagtttc tttatacggc
     4141 aacctctttc catcctcctt gcccctacgc cccaccgttg ctttgtgtga tctctgttac
     4201 agaattggca gtaatgtgga gatgcgcaca taaaatcgcc acgatttttg caagcaacat
     4261 cacgaaattc cttacatgac ctcggtttag ttcacagaag ccgcgttctc atcctcccgc
     4321 ctcctccccc ataaaaaagc cagggggtgg aggatttaag ccatctcctg atgacgcata
     4381 gtcagcccat catgaatgtt gctgtcgatg acaggttgtt acaaagggag aagggcatgg
     4441 cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga ggtcgtggta tcgaaagata
     4501 tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta
     4561 aatcgacttt actgcgcatg attgccgggc ttgagacgat caccagcggc gacctgttca
     4621 tcggtgagaa acggatgaat gacactccgc cagcagaacg cggcgttggt atggtgtttc
     4681 agtcctacgc gctctacccc cacctgtcag tagcagaaaa catgtcattt ggcctgaaac
     4741 tggcaggcgc aaaaaaagag gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctac
     4801 aactggcgca tttgctggat cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg
     4861 tggcgattgg ccgtacgctg gtggccgagc caagcgtatt tttgctcgat gaaccgctct
     4921 ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac
     4981 gcctgggccg cacaatgatt tacgtcaccc acgatcaggt cgaagcgatg acgctggccg
     5041 acaaaatcgt ggtgctggac gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt
     5101 accactatcc ggcagaccgt tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc
     5161 tgccggtaaa agtgaccgcc actgcaatcg atcaagtgca ggtggagctg ccgatgccaa
     5221 atcgtcagca agtctggcta cctgttgaaa gccgtgatgt ccaggttgga gccaatatgt
     5281 cgctgggtat tcgcccggaa catctactgc cgagtgatat cgctgacgtc atccttgagg
     5341 gtgaagttca ggtcgtcgag caactcggca acgaaaccca aatccatatc cagatccctt
     5401 ccattcgtca aaacctggtg taccgccaga acgacgtgga gttggtagaa gaaggtgcca
     5461 cattcgctat cggcctgccg ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat
     5521 gtcgtcgact gcatcaggag ccgggcgttt aagcacccca caaaacacac aaagcctgtc
     5581 acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat agaatgatga ttactctgcg
     5641 caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt
     5701 tgatttccac ggctatgcac gttccggtat tggctggaca ggtagcggcg gtgaacaaca
     5761 gtgtttccag actaccggtg ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta
     5821 tgctgaatta aaattgggtc aggaagtgtg gaaagagggc gataagagct tctatttcga
     5881 cactaacgtg gcctattccg tcgcgcaaca gaatgactgg gaagctaccg acccggcctt
     5941 ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg
     6001 ggcaggtaag cgcttctacc aacgtcatga cgttcatatg atcgacttct actactggga
     6061 tatttctggt cctggtgccg gtctggaaaa catcgatgtt ggcttcggta aactctctct
     6121 ggcagcaacc cgctcctctg aagcaggtgg ttcttcctct ttcgccagca acaatattta
     6181 tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat
     6241 caacccgggc ggcacattag aactgggtgt cgactacggt cgtgccaacc tgcgtgataa
     6301 ctatcgtctg gttgatggcg catcgaaaga cggttggtta ttcactgctg aacatactca
     6361 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
     6421 gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt gataacgaaa aatttgccta
     6481 caatatcaac aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
     6541 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
     6601 cggcaccaag tggtggaccg tcggtattcg cccgatgtac aagtggacgc caatcatgag
     6661 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
     6721 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
     6781 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt acgactacac
     6841 cggtaacgct gataccaacg cgaacttcgg caaagccgtt cctgctgatt tcaacggcgg
     6901 cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gcccagatgg aaatctggtg
     6961 gtaatagcaa aacctgggcc ggataaggtg cttgcgccgc atccggcatc caacgcctga
     7021 tgcgacgctt gcgcgtctta tcaggcctac aacggctgtc aaatgtaggc cggataaggc
     7081 gtttacgccg catccggcat aaaaacaggt tgtcattatc tgaaaggggc gcaagcccct
     7141 ctgattatcg ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg
     7201 aaaacaatga aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttgctggca
     7261 agcgcgcctg gaatcagcct tgccgaagtt aactacgtac cgcaaaacac cagcgacgcg
     7321 ccagccattc catctgccgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa
     7381 acccagacca cccaactggc gactggcggc caacaactga acgttcccgg catcagtggt
     7441 ccggttgccg cgtacagcgt cccggcaaac attggcgaac tgaccctgac gctgaccagt
     7501 gaagtgaaca aacaaactag tgtatttgcg ccgaacgtgc tgattcttga tcagaacatg
     7561 accccatcag ccttcttccc cagcagttat ttcacctacc aggaaccagg cgtgatgagt
     7621 gcagatcgtc tggaaggcgt tatgcgcctg acaccggcgt tagggcagca aaaactttat
     7681 gttctggtct ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct
     7741 aaagcctatg ccaaaggcgt cggtaactcg atcccggata ttcccgatcc ggttgctcgt
     7801 cataccagcg atggcttact gaaactgaaa gtcaaaacga actccagctc cagcgtgttg
     7861 gtaggaccac tatttggttc ttccgctcca gctccggtta cggtaggcaa cacggcggca
     7921 ccagctgtgg ctgcacccgc tccggtgccg gtgaagaaaa gcgagccgat gctcaacgac
     7981 acggaaagtt attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag
     8041 gcgttaaaac tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt
     8101 atcagcagtg taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg
     8161 gtgcgtctcc tggcgcacct ttttttatgc ttcattcctg ggatatgagc gattttttat
     8221 agtacttcat ttcttcttca ctaagaatgt gcattatctc aatgcattat cagagattct
     8281 tttcctttcg ccggtagtgt ctggacgctc aggctacttt ttccagttat ttcatttctg
     8341 taatgcagag gttttatgtt aggtcatatc tcaaagtttg acggcaataa ctctttgata
     8401 aaa
//
LOCUS       DAZUGK010000189         8383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZUGK010000189 DAZUGK010000000
VERSION     DAZUGK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.190"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..344
                     /locus_tag="ACHY5W_003822"
     CDS             <1..344
                     /locus_tag="ACHY5W_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFS4448630.1"
                     /translation="RIILPAKPDLNCVTRFGGVGLTPACEKGHLSIVKELLAHTEINV
                     NQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHSASPHLTDKYGKTPLELARERGFE
                     EIAQLLIAAGA"
     gene            384..1247
                     /gene="yahE"
                     /locus_tag="ACHY5W_003823"
     CDS             384..1247
                     /gene="yahE"
                     /locus_tag="ACHY5W_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS4448631.1"
                     /translation="MWALTADADFLARRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1237..2784
                     /gene="fdrA"
                     /locus_tag="ACHY5W_003824"
     CDS             1237..2784
                     /gene="fdrA"
                     /locus_tag="ACHY5W_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS4448632.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLLEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2784..4202
                     /gene="yahG"
                     /locus_tag="ACHY5W_003825"
     CDS             2784..4202
                     /gene="yahG"
                     /locus_tag="ACHY5W_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS4448633.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFEQAINVVPGMTAKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            4228..4482
                     /gene="yahH"
                     /locus_tag="ACHY5W_003826"
                     /pseudo
     CDS             4228..4482
                     /gene="yahH"
                     /locus_tag="ACHY5W_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            4532..5482
                     /gene="yahI"
                     /locus_tag="ACHY5W_003827"
     CDS             4532..5482
                     /gene="yahI"
                     /locus_tag="ACHY5W_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFS4448634.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSEIQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5492..6874
                     /gene="yahJ"
                     /locus_tag="ACHY5W_003828"
     CDS             5492..6874
                     /gene="yahJ"
                     /locus_tag="ACHY5W_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4448635.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPFAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            7090..7164
                     /locus_tag="ACHY5W_003829"
                     /pseudo
     CDS             7090..7164
                     /locus_tag="ACHY5W_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7251..8300
                     /gene="yahK"
                     /locus_tag="ACHY5W_003830"
     CDS             7251..8300
                     /gene="yahK"
                     /locus_tag="ACHY5W_003830"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFS4448636.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHALGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2030 a         2132 c         2407 g         1814 t
ORIGIN      
        1 tacgaattat tttaccggct aaacccgatc ttaattgcgt aacccgtttt ggcggtgtcg
       61 gcctgacgcc tgcctgtgaa aaaggccatt taagtattgt aaaagagctt ttggcgcata
      121 cggagattaa cgttaaccag accaaccatg taggctggac gccgctactg gaagcgattg
      181 tgcttaatga tggtggtatt aaacagcagg cgattgtgca gttattgctg gaacacagtg
      241 ccagcccgca tctgaccgat aaatatggca aaacgccact ggaactggcg cgagaacggg
      301 gctttgaaga gattgcgcag ttactgattg ccgcaggtgc ataaaccggg aggcttgcta
      361 tcaacacacc agaaaaacgg tgtgtgtggg cgctaactgc ggatgcggat tttctggcgc
      421 ggcgggggca aggacaggtt gaacaggttt ttgccagagc ggtaaatatc gcactcccgg
      481 ctcgccagca gttgctgacg ctgctttgtg aagagtacga caatgcgcca aacagttgtc
      541 ggttggcact cactcacttt gatgatctgt tccggcatgg tgataaggtt cagtttgacg
      601 atcaaggtat tacggttggt caacatcttc atatagagat gagtcgttgt cggcgttggc
      661 tgtccccaac cttgcaaatg accgctgtga attttcacct tatcgcctgg caacagtggc
      721 acgacattat tcatcagcac ctgggggaaa atgaaaccct gtttaattat cgcggcgata
      781 atccgtttta tcaggcgtta aataaagaat tacatattaa acgacgggca gttattcaga
      841 cagtaaacga taaacaaaat atcgcctcag cggtcgccag tatgatgggc ttagggattg
      901 gccttacgcc atcagccgat gattatttaa caggtctggc acttatttta tttatttccg
      961 ggcatccggc gggaaaatac aaagaggaat tttatctcgg cttgcaacgc ggcagaaata
     1021 ataccacgtt attaagtgcc ataacgctgg aagccgcatt acaacaacgc tgccgggaaa
     1081 atattcatcg ttttattcac aacattattt atgacatccc tgggaacgca actcaggcaa
     1141 tagaaaaaat taaacatatt ggctccagtt ccggctgcga catgctgtat ggcatggccg
     1201 atggttgtgc gctgagccaa acctacggag ggaattatgt cagttaaaat agtcattaaa
     1261 ccgaatacct attttgattc tgtctcgctg atgtctatct ccacgcgtgc aaataaactc
     1321 gacggcgtcg agcaggcatt tgtggcgatg gcgaccgaaa tgaacaaagg tgtgctgaaa
     1381 aatttaggac tgctgacgcc ggagctggaa caggcgaaaa acggcgacct gatgattgtc
     1441 atcaatggta aatcgggggc ggacaacgag cagttgctgg tggagattga agaactgttc
     1501 aacagcaaag cgcaaagcgg ctcgcacgag gcgcgttacg ccactattgc cagcgccaaa
     1561 aagcatatcc cggaaagtaa cctggcggtg atttcggtca acggtctgtt tgccgctcgc
     1621 gaagcgcgtc aggcgctgca aaacgatctc aacgtgatgc tgttttccga taacgtctcg
     1681 gttgaagatg aactggcgct caagcaactg gcccacgaaa aagggctgct gatgatgggg
     1741 ccagactgtg gcacggcgat tatcaacggc gcggcgctct gttttggtaa cgccgtgcgt
     1801 cgcggcaaca tcggtattgt tggcgcatcc ggcaccggca gccaggagct gagcgtccgc
     1861 attcatgaat ttggtggcgg cgtttcacaa ctgattggca ccggcgggcg cgacctgagc
     1921 gagaaaatcg gcggcctgat gatgctcgac gccatcggga tgctggaaaa cgatccgcaa
     1981 actgaaatta tcgcgctgat ttcaaaacct cccgcgcctg cggtggcccg taaagtgctg
     2041 gaacgcgccc gcgcctgccg caagccggtg gtggtctgct tcctcggtcg tggcgaaacg
     2101 ccggttgacg agcaggggct acagtttgct cgcggcagca aagaagcggc gctaaaagcg
     2161 gtgatgctct ccggcgtgaa acaggaaaat ctcgacctgc ataccctgaa ccagccgttg
     2221 attgcggatg tgcgtgcgcg tctgcaaccc cagcagaaat acattcgtgg cctgttctgc
     2281 ggcggcacgt tgtgcgacga aaccatgttc gcggtgatgg aaaaacatgg cgatgtctac
     2341 agcaacattc agcccgatcc ggaattccgc ctgaaagata tcaaccgcag catcaaacac
     2401 accttcctcg actttggcga tgacgacttc accaacggca agccgcatcc aatgattgac
     2461 cccaccaacc gcatcagtcg cttgctcgaa gaggcgcgcg atccagaagt ggcggtgatc
     2521 gtgatggatt ttgtgctcgg atttggatcg catgaagatc cggtaggctc caccatcgag
     2581 gcgatcaaag aagcgaaagc gatcgccgct gccgaaggac gcgagttgat cattctcgcc
     2641 tatgtgctgg gtaccgatct tgatacgcca tcgttagaac aacaaagcca gatgctgctt
     2701 gatgccggag tgattctggc gagcagcagc accaataccg gattgctggc gcgtgaattt
     2761 atctgcaaag gggaggaagc ctgatgagcc agtcactgtt tagccaacca ttgaacgtta
     2821 ttaacgtcgg catcgccatg tttagcgatg acctgaaaaa gcagcatgta gaagtgactc
     2881 aactcgactg gacgccgccg gggcagggca atatgcaggt ggtgcaggcg ctggataaca
     2941 ttgccgattc gccgctggca gacaaaatcg ccgccgctaa ccagcaggcg ctggagcgca
     3001 ttatccagtc gcatccggtg ctgattggtt ttgagcaggc gattaacgtg gtgccgggca
     3061 tgacggcgaa aaccattctt cacgccgggc cgccgatcac ctgggaaaaa atgtgcggcg
     3121 cgatgaaagg cgcggtcacc ggagcgctgg tgttcgaagg actggcgaaa gatctcgacg
     3181 aggcggctga actggcggct tccggggaga tcaccttctc gccgtgccac gaacacgact
     3241 gtgtgggatc gatggcgggt gttacctcgg cctcgatgtt tatgcacatc gtaaaaaaca
     3301 aaacctacgg caacatcgct tataccaaca tgagcgagca gatggcgaag attttgcgta
     3361 tgggcgctaa cgaccagagc gtgattgacc gcctgaactg gatgcgtgat gtgcagggac
     3421 caatactgcg cgacgcgatg aaaattatcg gcgaaatcga tctgcgctta atgctggcgc
     3481 aggcgctgca tatgggcgat gagtgccata accgcaataa cgccgggacg acgctgctga
     3541 ttcaggcgct gacgccgggg attattcagg cgggctactc cgtcgagcaa cagcgcgaag
     3601 tgtttgagtt tgtcgccagc agcgactact tctccggccc gacgtggatg gcgatgtgta
     3661 aagcggcgat ggatgcggcg cacggcatcg aatacagcac cgtggtcacc accatggcgc
     3721 gtaacggcgt cgagttcggc ctgcgggtca gcggcctgcc ggggcaatgg tttaccggtc
     3781 cggcgcagca ggtgatcggc ccgatgtttg ccggttataa gccggaagat tcggggctgg
     3841 atatcggcga cagtgccatc accgaaacct acggtattgg cggatttgcc atggcgaccg
     3901 cgcccgctat cgtcgcgctg gtgggcggca cggtggaaga agctattgat ttctcccgtc
     3961 agatgcgcga aatcaccctc ggtgaaaacc ccaacgtcac cattccactg ctcggtttta
     4021 tgggcgtacc gtcggcaatc gacatcaccc gcgtgggcag cagcggcatt ttgccggtga
     4081 tcaacaccgc catcgcccat aaagatgcgg gcgtcggcat gattggcgcg ggcattgtgc
     4141 atccaccttt cgcctgcttc gagaaagcca ttcttggctg gtgcgaacgt tacggcgtct
     4201 gacctgcatg ttcatgccgg atgcggcgtg aacgccttat ccggcctacg aatggcgcaa
     4261 gaatctgtag gcctgataag cgtagcgcat caggcatttg tcaccattgc cggatgcggc
     4321 gtgaacgcct tatccggcct acaagtggcg caagaatttg taggcctgat aagcgtagcg
     4381 catcaggcat ttgtcaccat tgccggatgc ggcgtgaacg ccttatccgg cctacgagtg
     4441 gcacgagaat ttgtaggcct gataagcgta gcgcatcagg caatttggcg ttggtcataa
     4501 ccccatcacc ctctgtagcg gacataacaa catgaaagag cttgtggtcg ttgccattgg
     4561 tggcaacagc attatcaaag ataacgccag ccagtcgatt gagcatcagg cggaggcggt
     4621 gaaagccgtc gccgatacgg tgctggaaat gctggcttcc gattacgaca ttgtgctgac
     4681 ccacggtaac gggccgcagg tcgggctgga tttacgccgt gcggagattg cccacgagcg
     4741 cgaagggctc cccttaacgc cgctggcgaa ctgtgtggcg gatacgcaag gtggcattgg
     4801 ctatctgatc cagcaggcgc tgaacaatcg actggcgcgc cacggcgaga aaaaagccgt
     4861 caccgtggtg actcaggtgg aagtggataa aaacgatccg ggatttgccc atcccaccaa
     4921 gcccatcggc gcattcttca gtgaaatcca gcgtgacgaa ttacaaaagg caaaccctga
     4981 ctggcgtttt gttgaagatg ccggacgagg ctatcgccgc gtggtcgcct cgccggaacc
     5041 gaaacgtatt gtcgaagcac cggccattaa ggcgctgatc cagcaaggct ttgtggtgat
     5101 tggcgcgggc ggtggcggaa ttcctgtggt gcgtactgac gcgggagatt accaaagcgt
     5161 ggacgcggtt atcgacaaag atctctctac cgcgctactg gcccgtgaaa ttcacgccga
     5221 cattcttgtg atcaccactg gcgtggaaaa agtgtgtatt cactttggca aacccgagca
     5281 gcaggcgctg gatcgcgtag atattgccac tatgacccgc tatatgcagg aagggcattt
     5341 cccgcccggc agcatgttgc caaaaatcat cgccagcctg acgttcctgg aacagggcgg
     5401 taaagaagtg attatcacca cgccggaatg cctgccagcg gcgctgcgcg gtgaaacggg
     5461 cacccacatt attaaaacgt aaggacgtaa gatgaaagaa aacaatagcc gccgtgaatt
     5521 tctgagccag agcggtaaga tggtcaccgc cgccgcgctg tttggtacct ctgtgccgtt
     5581 cgcccatgcg gcggtctctg gcaccacaaa ctgcgaagcg aacaacacca tgaaaatcac
     5641 tgacccgcat tactatctcg acaacgtgct gctggaaact ggatttgact acgaaaatgg
     5701 cgtggcggtt cagacccgca cggcgcgcca gaccgtggag attcagaacg gcaaaattgt
     5761 cgcgctgcgc gagaataagc agcacccgga tgccacgctg ccgcactatg acgctggcga
     5821 taagctgatg ctgcccacca cccgcgacat gcatattcat ctcgacaaaa ccttctacgg
     5881 cgggccgtgg cgctcgctca atcgtccggc aggcaccact atccaggaca tgatcaaact
     5941 cgagcagaaa atgctgccgg agttacaacc gtatacgcag gagcgggcgg aaaaactgat
     6001 cgatctattg cagtcgaaag gcaccaccat tgcccgcagc cactgcaata tcgaaccggt
     6061 ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg gcgcggcgtc aggcgggctt
     6121 cgagtgtgaa atcgtcgcct tcccgcagca cggtttgctg ctgtcgaaat cggaaccctt
     6181 aatgcgcgaa gcgatgcagg cgggggcgca ttacgtcggc gggctggacc cgaccagtgt
     6241 cgatggcgcg atggaaaaat ccctcgacac catgttccag attgcgctgg actacgacaa
     6301 aggcgtcgat attcacctgc acgaaaccac tccgtcgggc gtggcagcca tcaattatat
     6361 ggttgaaacg gtagagaaaa cgccgcaact gaaaggtaag ctgaccatca gccacgcctt
     6421 tgcgctggct acgctcaacg aacaacaggt cgatgcgctg gcgaatcgga tggcggcgca
     6481 gcaaatttct atcgcctcga cggtgccgat tggcacgctg catatgccgc tcaaacagtt
     6541 gcacgacaaa ggcgtaaaag tgatgactgg cactgacagc gttatcgacc actggtcgcc
     6601 ttatggtctg ggcgacatgc tggaaaaagc caatctctac gcgcagctct atattcgtcc
     6661 taacgaacag aacctctccc gttcgctgtt tttagccact ggcgatgtat tgccgctgaa
     6721 tgaaaaaggc gagcgtgtat ggccaaaagc gcaggatgac gccagctttg tgctggtgga
     6781 cgcctcctgt tccgccgagg cggtggcgcg tatctcgccg agaaccgcaa cgttccataa
     6841 agggcaactg gtgtggggga gtgtggcagg ttgatgcgca tttgaaacgc ctgcaacggt
     6901 gaatagtaag agatttaagc cccagggagt ggggcaatct gaatatgagt gtgtcggcag
     6961 aaatggacat tatctttgag gaatatgccc ttattgacgt tgtgtagata actatttgtc
     7021 tacgtgaaaa gatcatcagt tttgccgatt ttagtctttt acggatagca aatatcacac
     7081 ttataggcgc tcgccttatg gggaacgttg ctgctctgtt tcattcaggc tcgcggcatg
     7141 ttggggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt tttgccgcga
     7201 tgacatatca ggcatcgcca aatacacata gctaatcagg agtaaacaca atgaagatca
     7261 aagctgttgg tgcatattcc gctaaacaac cacttgaacc gatggatatc acccggcgtg
     7321 aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtctgc cattccgatc
     7381 tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtgccg ggtcatgaaa
     7441 ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa acatgcgctg ggcgatctgg
     7501 tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa gacgggctgg
     7561 aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac gaaccgggcc
     7621 atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt ctgcgtattc
     7681 gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg atcaccacgt
     7741 attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg
     7801 gcggtctggg acatatgggg attaagctgg cccacgcgat gggggcacat gtggtggcat
     7861 ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat gaagttgtta
     7921 actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc attttgaata
     7981 cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt gatggcacca
     8041 tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc aacctgatca
     8101 tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact caggagatgc
     8161 tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa
     8221 ttaatgaagc ctatgagcga atgctgcgcg gtgatgtgaa atatcgtttt gttatcgata
     8281 atcgcacact aacagactga aaaaattaat aaataccctg tggtttaaca tattaacttc
     8341 gctctccact taacttttta gttaaggaga gcgaaataat atc
//
LOCUS       DAZUGK010000190         8355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZUGK010000190 DAZUGK010000000
VERSION     DAZUGK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.191"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2020)
                     /locus_tag="ACHY5W_003831"
     CDS             complement(<1..2020)
                     /locus_tag="ACHY5W_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4448637.1"
                     /translation="MATLRELIIKISANSQSFQSELQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRSSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQR"
     gene            complement(2071..2280)
                     /locus_tag="ACHY5W_003832"
     CDS             complement(2071..2280)
                     /locus_tag="ACHY5W_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFS4448638.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAMS"
     gene            complement(2376..2750)
                     /locus_tag="ACHY5W_003833"
     CDS             complement(2376..2750)
                     /locus_tag="ACHY5W_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS4448639.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLFDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2765..3481)
                     /locus_tag="ACHY5W_003834"
     CDS             complement(2765..3481)
                     /locus_tag="ACHY5W_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4448640.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(3540..3884)
                     /locus_tag="ACHY5W_003835"
     CDS             complement(3540..3884)
                     /locus_tag="ACHY5W_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFS4448641.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3881..4327)
                     /locus_tag="ACHY5W_003836"
     CDS             complement(3881..4327)
                     /locus_tag="ACHY5W_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4448642.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4324..4674)
                     /locus_tag="ACHY5W_003837"
     CDS             complement(4324..4674)
                     /locus_tag="ACHY5W_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4448643.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(4685..5011)
                     /locus_tag="ACHY5W_003838"
     CDS             complement(4685..5011)
                     /locus_tag="ACHY5W_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4448644.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5008..7268)
                     /locus_tag="ACHY5W_003839"
                     /pseudo
     CDS             complement(5008..7268)
                     /locus_tag="ACHY5W_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(7214..7435)
                     /locus_tag="ACHY5W_003840"
     CDS             complement(7214..7435)
                     /locus_tag="ACHY5W_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448645.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(7480..>8355)
                     /locus_tag="ACHY5W_003841"
     CDS             complement(7480..>8355)
                     /locus_tag="ACHY5W_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4448646.1"
                     /translation="AQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAG
                     WVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVAR
                     LDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWL
                     MSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLAD
                     DGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAA
                     VAVITGVNYGSASGG"
BASE COUNT         1720 a         2770 c         2033 g         1832 t
ORIGIN      
        1 aacgttgtct cgcctcctga tacggatcac ccacaaaacc gaggtcatcc gccgcatacc
       61 ccagccggac acgctctttt tcttcccctt tcagtctgga cagggccagc tcacgctctg
      121 tttttgtcag ggcactctgc tgtttatcat ccagagtggc ctgtggcagc cgtaacggca
      181 cattcaccag tccctgccgc tgctgaagca gttcattacc cagccccagc agacggttga
      241 attccgtatg ctgaccattc ataaccagca tggactggta caccttattc tgctctgccg
      301 cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca tcctgaatgg
      361 actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc tgctctgttg
      421 cctgagcaag cccttccgtt acggtcttca ccgaggtcag atggtttatc atgaatccgt
      481 caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt ttttcctgca
      541 gggatttcac ccggctggcc tgttcatcaa tcagccggtt ctgctctgtc agcgccgccc
      601 gtgttcgtcc ttcattatct gaggcttcag gcaaagacat tgacggcgtt ttatgcgcga
      661 tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc tgattctggt
      721 acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca ccaaccagcc
      781 ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc gcattctggg
      841 cggcgcttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc agacgggcct
      901 ctgctgcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga gcacggtaca
      961 cggctgctct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct tccgccagtg
     1021 caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct ccggaggcca
     1081 tattgccaaa gtaccgggca accccgacgg caaccagtgc ccccacggct gttgccacat
     1141 tatcaatctg tccggcaaca ccgttcagca cgccggagag cgttttcgtc accccgctgg
     1201 cctcattcgc accgcccacc caggccataa aggcgttttc caccttcgtg atactactgg
     1261 aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg ctgattaacg
     1321 cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc agatctttac
     1381 gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt tcattgaccg
     1441 aattaaactc ctcaccacgc aatacaccct gcgccagcgc ctgactgaac tgggtgatca
     1501 ccgagcccgc ctctgccgta ctggcaccgg agattttcag ccctgtcgaa atggcctccg
     1561 tcaccttcag cacatcatca gcactgtaac catattcacg cattgaggcg gctgagcggg
     1621 caaacagggc cgcattatcc gaaaatgcgg tgcctgtccg ctggctgata tccatcagca
     1681 ctttctgtga tgacgaaaat tcatcagatg actgcgacgc ctgtttcaga cgggcattca
     1741 cggaactcca ctcatccgcc agtgaaatca ggtgtccggt ggcaaaggca ccggcaaatg
     1801 cgccagccat tccgacagca gaactgcgaa tttccgtcaa ctggctgttc agttctgcca
     1861 gggcacgtcg ctgctcccgg gctgccgcag cggcctgacg cccgccattc tgcagggtcc
     1921 ggtaatattc actgcccata cgggaagcac gctggagctc cgactggaat gactgtgaat
     1981 ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct ccgggcaaaa
     2041 aaacctgcca cagcaggttt tcatcattat ttatgacatt gctgcaaagc tcagcgcgtc
     2101 ttccagcgcc gcaaacggat ccacctccgg cttatcctca tcctcgcccc agcagagcat
     2161 ggcgtcctcc agtgaaacat tcatcccctg cgccccgaaa accgctttca cgatctgcgc
     2221 attacggata tccccgcgct catcgcccag cggggacacc ctgtcgaact ccatccacat
     2281 catcgcctcg ctcgcactca gactgtggcc cagttcggat aaggtgcgcc ccagacggag
     2341 cgcaagtcgc atcagaaaga gaatttccgg gcgggctact tttttctggc cgactctgca
     2401 tcagcgatca gttccagtgc ctgacgcagc aaccgggcat gtaccggacc atagacggcc
     2461 agcacctgct cacggtcgtc cggagtgaac acccgctgca gatccgtatc aaacaggaca
     2521 tcgcagaaca gcgtcacatc cgcttccagg ttacggcggg ttttcgccac caccgacagg
     2581 gtatcgtcat cctctccatc accattgagc acttcctgcc acagatacca ggcctctgcc
     2641 gaaggctccc gcagcaccac gctgacatta ccccattccg gcaccttcac cgttttatga
     2701 cgaaaccctg acagtctggc cagcgccagc gttttcagat cctttttcat gatgacccat
     2761 ccccttatcc ggcggctgcg ctcactgtca cggtgcattc aacagacgtc acactctgtg
     2821 ctttctctgc cgaatcggtc accacgcagg tatatttccc cgcatcagcg gactgcgcac
     2881 ctggcttact gaaggtgtct gtcgtctgcc cgtcaaccgg ctgaccatcc ttcttccagg
     2941 cgtatttata cggcggcgtt cccccgttgg cactgactga cattgtcagc agcgcacctg
     3001 tattcacggt aagtgtttta tccagatttt tcacaaacgc cagcggtacc acaaaggaca
     3061 ccggtttgcc tttcatacgc agtgaaaacg ttgctgccac cacgccgttg gtaccggatg
     3121 accaggtgtg ctgacgcact tccgccagga acttaaagcc cttaccggac ggaaacagca
     3181 ccttaaacgc atacaacgcg tcattgtcat aggcatcacg cagggcgttc tgggcctgat
     3241 tcagataaaa attacccgac atggaaatct cagacgacgc ccccagaccg ttgatgttct
     3301 cctgctcggt ggagcagagc gtggtcacat caatatcctg tttctgaccg gcggtgaact
     3361 ggacttcctt gatggtgcag tccaggcgca gatattccgc cttatccata gtttcagcag
     3421 tcgccggggc agatgaaatc atcacctgcg tcagctgtga gcgttcatac aaagcagaca
     3481 ttctgcctcc tgataataaa aaacccgcac gcggcggggt atgggttctg ttgaaaaaat
     3541 tacaccgtga cctgaaactc cagggttgca cggtaacagc ggttttccgg aatatagtcc
     3601 tgcatttcac tgacggatcc cggggccagc agcattatgg cttcacgggc gtcctgacgt
     3661 atctgacgcg cctgcgtcac agtcccggca taaacgtcta tctgcaccga cactgaggac
     3721 tccgcctgcc cgcccatcac gtccgcagac accgatgaaa tcaggctgaa aaccacccac
     3781 ggaagcgcca ccgacggcct gccatccagc agggggacca catacgggta cacctgcccg
     3841 ccggcaagat gcgccagatg aggatacaaa tccgcctccg tcatcgtctc agtacctcat
     3901 caatggcccg gttcatccgt gcaatcgcca cttgcgctgc ctgttcactg cgcacatcaa
     3961 acgccggacg cacaaacggg tgcggtggca tattcacggt ccccatttcc acaaaccgcc
     4021 agtagaaagc attgcgcggg ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg
     4081 gattaacacc ccggatatgc acaccggatt ccatcccgcc atcgcgggag cgccgggaaa
     4141 ggaccaccac attgcggcgc agttttcccc tgcgtaccgg tgcccgtgac accacttctt
     4201 ctttcagcac attcgcaccc gcacgggttg cctcacgcag cacccggtta ttttctgcac
     4261 cactcagaag ctgcaaatcg cggctgatgt cctccagccc cgaaaaatcc agcagggttt
     4321 cgatcatttt tcacctccca gccgacagag aatttccaga cgtccgccgg tcgcatccgg
     4381 cacgggcagc ccgacaacgt tcaggatccg gtcacgccag ggaccactca gcacatgaag
     4441 tcgtgacgct gccgtgattt cccgaccgga ctgaccgcgc acccagatgc ggatttccgc
     4501 ctgcgccatt tccgcaccgg actgcatccg ctcccggctg ctcctgccac ggatatccgc
     4561 atgaattttc ccgcatgaca cccattcttc cgtcatttct ccggcagcat tacgggttaa
     4621 caccgggttc agaacactta tcatctgtgt cagacgacct gcagatattg ccattcctcc
     4681 ctcctcataa caccgtcgga caacgcaaat cgtaaatcag cacggacaca gaaaacggca
     4741 gttccccctg cacgaggtct tcccgctcag caagatccgg attccggtac agcatcccgg
     4801 tcagtcgcat ggcagccccc ttcatccggg ttaatgcctc gcccgggatc agctcaccgt
     4861 cctcacgaat cactttatcc cggctgccct gaatgtaggc cagcagcacg gcggtagcct
     4921 gacgaacctt gtccatcagc atgtcatcat ccgcgtcatg gtcaacacgc agatgtgcct
     4981 tgatctcttc cagtgtcagt aatgccgtca ttttccgcct cctgcatccc gtccacgttt
     5041 tgcagccagg gtccagcctg atgaatgagc ttctccgggt ttatcaccgg tcatactgtt
     5101 gcagtgccac agcgagcccc cccacgtcac cgtatcgccg gggtggtagg tttcaccggc
     5161 tctgaacaca ccgcggtaga gcatcaccgg cagggaaaat gttttttccg tacactggcc
     5221 actgctctgc cggatcacca cagagaacaa ccgctcatcc gtcatgctga cgtcgatatc
     5281 cgccaccccg tcaaccaggc attcccatcc ccgcatcccg tgcgtttttt catacgcccg
     5341 ccagagtcca cccaggtgtg tggcatacgt gccccgggga aaggattttt gatcgtcaat
     5401 agcggggagc acttccagtg ccgtggcatc acgcccgtcc tgcggagccg gaagggcatt
     5461 caccgcctcc agaaccgcct gcttcagtac ttccggatcg taatcacgac catcacgcgg
     5521 agcagggata tggcttacgg cctctttcac catctgctca agcatcggac gcacatcatc
     5581 gggggtgata cttttgccgt ccgccggtac cggaatattc gcaaccgcat cattcaccgc
     5641 ctgctgcagt acatccggat cataatcacg accatcacgc ggtaccggaa tggtccccac
     5701 agcgtcatcc accatcgcct gcagaaccgg atgtacctca tccaccgtca catgcttctg
     5761 taataccgcc gacagggaag ccagtttctc ttcaaacgct tgtgcctgcg cggccatctt
     5821 cccctcaaat gtgcgctgta aatccgccag caccgtggag aattcttcgc ccagtgcacg
     5881 aataatggac agttcccgtt ccgtcatttt ctcagtatcc ccctgaacat cgctttcact
     5941 gcatcatgct ctgtttcact gattgcctta ttaccgtcag atgcgccgtc aggcagttgt
     6001 gctgaaactg ttttcccggc cgacgcgaac gggtcctcac gggcatcacg acgggacagc
     6061 gcctccagac tgtagttctg ctgctgaaga tacagtgcat caccgccggc aaggggcggc
     6121 aggttctcac gtttacgggc ctcattgggc gtgagaagcg tatttttcac cgattcaccc
     6181 agtgttttca tgcgccgttc gctgtccatt ctcagcagcg tggtgacgtc aaactccgtg
     6241 ctctcgtttt cccccgtttc cagcgcctca tccagtaaca gctcaatgga ctcaatcagc
     6301 gtctgcagac actgggaata atactgctgc tccagcgcct ccacgttgtc actggaaggc
     6361 gggtggccaa cgccaatctt gtaggccggg acacggaaca ccgaacagac aatttcagcg
     6421 gtcatcttca gttgttccac cgtctgcgca tccacaggtg aaaacgtcgt ggggttgtat
     6481 tttgccccgt tgctcagaat ggccgttttc cccgcatttt cgcctgtata cccgctgtcc
     6541 cagttgctct tcagtttttt cgcattttct tccgtaatac tgccggggat ctcaatcacc
     6601 ccggacggcc tgccgccatt tctgaaaaaa gacgtcgaat tttcctgaat atgatgcccc
     6661 tgcgtggccg ccagcccggc ggcatacacc ggcggcaatc ctataagcgg atgaaaaaaa
     6721 cagttaaacc ggtcgtggat cacttcccgg gcaggcaccg tcaccgcctc cgtgatcccg
     6781 cagttccggt ccggcgtgat gcggtagaac acctcgccgt catccgccac cagaggttca
     6841 acccggctcc agtccagaat acgcagttct ttgatctgcc cccgggcatt acggattttc
     6901 agcaccaccg tattgccatg acgcagtttg gcgttcagcc acagttcaaa aaactggatg
     6961 cggttctgct gggcgttggg acgacgacag aggcgggcaa tatccccccg gcgcgtttcc
     7021 ctgcgtatcc catgcgcatc cgtctgcatc agacgcagcc gcattttggc gatatcctgg
     7081 gatatcagcg aaatacatgc aaacaccgca tgaaaggaga ggacggcttc aggatcggct
     7141 ttcacgccct gctgccaggc gccggaaaag ggctcagcca ccgcctgaaa caggctggtc
     7201 cagcccgcct ctcttacgtc acgtcctgat ttctggtttt ttcgggttcg ccgtaaaagg
     7261 ttccacattc gccatgctcc gcatcacgtt tctttttctg acctgccgga cgtcgcaccg
     7321 tgatgtactc cgcctttccc aggcgaacca gcacctccgc acacggctgt gccacatcac
     7381 ggatatcccc ggcccgggca tcatgcgtgc cctgcagata tcggatcttt gccataacct
     7441 gttacgggag gcgcacgcct cccgtcctcc ttatcagact cagccgccgg acgcactgcc
     7501 gtagttcact ccggtgatca ccgccaccgc cgcagtacgg cgacgacgcc agttgatcca
     7561 gcgctccgca cggatggcca cgctgcctgt ctggaacatg gaaaccagct ccaccgggga
     7621 cggcgtggta ctgtcgccgg tcggctcaga ctgcatttcc agtgatgcct cgcgggacat
     7681 atccactgcc acgccgccgt catccgccag ataaatatcc ggggcattca ccagcaccag
     7741 ctggtcaccc acgtactggg agacaatcac cggcagcccc tggaaggagc cacccagcag
     7801 ggtcatgtcc gggtattcct tctgacccag cgcattttta cgcatggaca gtgccagggc
     7861 attggtgctg gacatcagcc agaccgcacc ggtgggctgc aggtttgctg ccacaaactg
     7921 tccaaacgca gcctctgcat ccgcatccgg gttaccggtt gatgccgtgc ccttcacatc
     7981 atgggtgatg gacgccgggg agacatctgc cactgcggct tttttcgggt ccacaaagtc
     8041 tgtatccaga cgcgccacca ccgcttccgc cagcgcatta cggaccagtg catcagcagc
     8101 cggactggaa aaacggatca attcttccgt cagtaccgca atggccgaca ccttcgcatg
     8161 actgaaggtg atggattcaa aatcaaactt cgtcaggggt tttgccttac cctcacccac
     8221 ccagccggca gcaccgccgg acacctgggc gtgcacacgg atattgaatg gcacctgacg
     8281 aagtgcaggg atcccgccct gaccaaatcg cccgataatg gtctgcggac gcaggtaatc
     8341 aataaagtcc tgtgc
//
LOCUS       DAZUGK010000191         8354 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZUGK010000191 DAZUGK010000000
VERSION     DAZUGK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.192"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..1801
                     /gene="iucA"
                     /locus_tag="ACHY5W_003842"
     CDS             77..1801
                     /gene="iucA"
                     /locus_tag="ACHY5W_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFS4448647.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1802..2749
                     /gene="iucB"
                     /locus_tag="ACHY5W_003843"
     CDS             1802..2749
                     /gene="iucB"
                     /locus_tag="ACHY5W_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFS4448648.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2749..4491
                     /gene="iucC"
                     /locus_tag="ACHY5W_003844"
     CDS             2749..4491
                     /gene="iucC"
                     /locus_tag="ACHY5W_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFS4448649.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4488..5825
                     /gene="iucD"
                     /locus_tag="ACHY5W_003845"
     CDS             4488..5825
                     /gene="iucD"
                     /locus_tag="ACHY5W_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFS4448650.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRLDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            5831..8026
                     /gene="iutA"
                     /locus_tag="ACHY5W_003846"
     CDS             5831..8026
                     /gene="iutA"
                     /locus_tag="ACHY5W_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFS4448651.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1960 a         2135 c         2236 g         2023 t
ORIGIN      
        1 ataacatttc ttattgataa tgagaatcat tattgacata attgttatta ttttactgtg
       61 tgggagctgt ttgactatga ccctgccctc tgaaaaacca gccacagatg tggctgcgca
      121 gtgcttcctg aatgcactga ttcgtgaaac tacagactgg aaactgacag aatacccgcc
      181 agacgaattg atcatcccgc tggatgagca gaaatcgctc catttcagag tggcttattt
      241 ctccccaacc cagcatcacc gctttgcatt tcctgcacgt ctggtcacgg catcaggcag
      301 ttatcctgtc gactttacca ctctctcccg gcttattatt gataagctac ggcatcaact
      361 gtttctgccc gttcccctct gcgaaacttt ccaccagcgc gtgctggaaa gccacgccca
      421 tacgcaacag gcaattgatg cccgtcatga ctggaccgcc ctgcgtgaaa aagcgttgaa
      481 ttttggcgaa gctgagcagg cgctgctgac aggacacgct ttccaccctg cgcctaagtc
      541 tcatgaaccg tttaaccggc gggaggctga acgctacctg cctgatatgg caccccactt
      601 cccactgcgg tggttttcgg tggataaaac gcaaatcgcc ggtgaaagtt tgcatctcaa
      661 ccttcaacag cggctgacgc gatttgccgc agagaatgcg cctcagttac tcaacgaatt
      721 aagtgacaac caatggctgt tcccgttgca cccgtggcag ggggaatatc ttttgcagca
      781 ggggtggtgc caggcacttg ttgctaaggg gctgattaaa gacttaggtg aggccggcac
      841 gtcgtggctg ccgaccacct cttcccgttc cctctactgt gccaccagcc gcgatatgat
      901 caagttctcc ctgagcgtac ggctgaccaa ctccatccgt actctgtccg tgaaagaagt
      961 gaagcgtggg atgcgcctgg cacgcctggc tcaaaccgac ggctggcaga tgctacaggt
     1021 ccgcttcccg actttccggg taatgcagga ggatggctgg gccgggctgc tcgatcttaa
     1081 cggcaacatc atgcaggaaa gtctgtttgc cctgcgtgaa aatctgctgg tggatcagcc
     1141 gaaaagccag accaacgtac tggtctccct gactcaggcc gcacctgatg gcggtgattc
     1201 gctgctggtt tcggcggtaa aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc
     1261 cgcccatgca tgggtcgatg catactgtca gcaagtgcta aagccgctgt ttacggctga
     1321 agcggattac ggcctggtgc tgctggcgca tcagcaaaat attcttgtcc agatgcttgg
     1381 ggatctgccg gtcggattta tttaccgtga ctgtcagggc agcgctttta tgcctcacgc
     1441 gacagactgg ctcgattcta ttggcgaggc gcaggcggaa aatattttca cccatgagca
     1501 gttgctgcgc tatttccctt attacctgct ggttaactcc acttttgctg tgaccgctgc
     1561 gctgggggct gccgggctgg acagcgaatc gaatctgatg gctcgtgtac gagcatcgct
     1621 ggctgaagtg cgtgatcagg tgactcataa aacctgtctc aactacgtgc tggaaagtcc
     1681 gtactggaac gtaaaaggta actttttctg ttatctgaac gatcataacg agaacaccat
     1741 tgttgaccct tcggtgatct atttcgattt cgctaacccg ctgcaggctc aggaggtctg
     1801 aatgtctgag gcaaacatta ttcacagcag atatggactg cgctgtgaaa aactcgacaa
     1861 gcccctgaat cttggctggg gactggacaa tagcgcggtg ttgcactgtc ccggggagct
     1921 gccgacaggg tggctgtgcg atgcgctgga tcagatattt atcgccgcac cacaactgtc
     1981 agcagtggct ctcccctggg ccgaatggtg tgaggagcca caggcgctga cgctttttgg
     2041 gcaggtaaaa agcgacatta tccatcgtac cgccttctgg cagttaccgt tatggttgag
     2101 ttcaccggca aaccgggcct ccggtgaaat ggtttttgat gcagagcgtg agatttattt
     2161 cccgcagcgc ccaccccgtc cgcagggtga ggtttaccgc cgttacgatc cacgtattcg
     2221 caggatgctg agtttccgca ttgccgatcc cgtttctgat gcagaacgtt tcacccgctg
     2281 gatgaatgat ccgcgcgttg agtatttctg ggagcaaagt ggctcactgg aggtacagac
     2341 cgcctatctg gagcgccagc tcaccggtaa acatgcattc ccgctgattg ggtgcttcga
     2401 cgatcggcca ttcagctatt ttgaaatcta ctgggcggcg gaagaccgca ttggtcgtca
     2461 ctattcgtgg cagccctttg accgtggcct gcatctgctg gttggtgaac agcaatggcg
     2521 cggggcccac tatgtgcaaa gctggctgcg cgggctgaca cattacctgc tgctggatga
     2581 gccccgtacg cagcgcacag tactggagcc acgcaccgat aaccagcgcc tgttccgcca
     2641 tcttgagcct gcgggatacc ggacaattaa agagttcgac ttcccacata agcgctcgcg
     2701 catggtgatg gcggatcgtc atcacttctt cacggaggtc ggtctgtaat gaatcacaag
     2761 gactgggatt ttgttaaccg tcggctggtg gcaaaaatgt tgtctgagat ggagtatgag
     2821 caggttttcc acgcggaatc tcagggcgat gaccactact gcattaacct gccgggagca
     2881 caatggcgct ttatcgctga acgtggtatc tggggctggc tgtggattga tgcccaaacg
     2941 ttgcgctgca cggacgaacc agtactggcg cagacgctgc tgatgcagct aaagccggtg
     3001 ctgtcaatga gtgatgccac tgttgcagag catatgcagg atttgtatgc cacgctactg
     3061 ggcgacctgc aactgctgaa ggcccgtcgc ggactgagtg ccagtgacct gattaatctt
     3121 gatgccgatc gtctgcaatg cctgctgagc ggtcatccta aattcgtttt taataaaggt
     3181 cgccgtggct ggggcaaaga ggcgctggaa cgatatgcgc cagagtatgc caacaccttc
     3241 agactgcact ggctggcggt aaaacgtgaa catatgatct ggcgctgcga caacgatctg
     3301 gatattcagc agttgttgac tgccgcaatg gatccgcagg agtttacacg cttcagtcag
     3361 gtctggcagg aaaacgggct ggatcacaac tggctgccgc tgccggtaca tccgtggcag
     3421 tggcagcaaa aaatcgccac cgacttcatc gctgattttg ccgaaggcag gatggtgtct
     3481 ctcggcgagt ttggcgacca gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc
     3541 agccgccggg gagggctgga tatcaagctg ccgctgacca tctacaacac ctcatgctac
     3601 cgggggatcc ctggcagata tatcgctgcc ggaccactgg cttcacgctg gctgcaacag
     3661 gtttttgcga ccgacgccac cctggtgcaa agcggcgcag tgatccttgg tgaaccggct
     3721 gcaggctatg tgtcccatga aggctatgcc gcgcttgccc gggctcccta tcgctaccag
     3781 gaaatgcttg gtgttatctg gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt
     3841 ccggttctga tggcaacact gatggagtgc gacgaaaaca atcagccgct ggcaggcgca
     3901 tatatcgacc gctccggact ggacgctgaa acctggctta cgcaattgtt ccgggtggtt
     3961 gtggttccgc tgtatcacct gctttgccgc tacggtgtcg cgcttattgc acacggacaa
     4021 aatataacgc tcgccatgaa agagggggta ccacagcgtg ttctgctgaa agacttccag
     4081 ggcgatatgc ggctggtgaa agaagcgttt cccgaaatgg actctttgcc tcaggaggtg
     4141 cgtgatgtca catcccgtct gagtgcggac tacttaatcc atgatttgca gacgggtcac
     4201 ttcgtgacag tactgcgttt tatttcgcca ctgatggttc gtcttggcgt acctgaaagg
     4261 cgattttatc aactgctggc agcagtgttg agtgattaca tgaacaaaca tccacaaatg
     4321 tcagagcgtt ttgcgctttt ctcactcttc aggccacaaa tcattcgcgt ggtgctgaac
     4381 ccggtaaaac tgacctggcc ggaccaggat ggcggcagtc gcatgctgcc gaattacctt
     4441 gagaatctgc aaaatccgct gtggctggta actcaggaat atgaatcatg aaaaaaagtg
     4501 tcgattttat tggtgtcggg acagggccat ttaatctcag tattgctgca ttgtcacatc
     4561 agatcgaaga actgaactgt ctcttctttg acgaacatcc tcatttttcc tggcatccgg
     4621 gtatgctggt accggattgt catatgcaga ctgtctttct gaaagatctg gtcagtgcag
     4681 ttgcacctac aaatccctac agctttgtta actatctggt gaagcacaaa aagttctatc
     4741 gcttccttac aagcagacta cgtacagtat cccgtgaaga gttttctgac tatctccgct
     4801 gggctgctga agatatgaat aacctgtatt tcagtcatac cgttgaaaat attgatttcg
     4861 acaaaaaaag tcgattgttt ctggttcaga ccagtcgggg agaatatttt gcccgcaata
     4921 tctgccttgg tacaggaaaa caaccttatt taccaccctg tgtgaagcat gtgacacaat
     4981 cctgtttcca tgccagtgaa atgaatcttc gtcggctgga cctgagtgga aaacggataa
     5041 ctgtggttgg tggaggacag agtggtgcag acctgttcct taatgcatta cgcggggaat
     5101 ggggagaagc ggcggaaata aactgggtct cacggcgtaa taattttaac gcactggatg
     5161 aggctgcttt tgctgatgag tattttacac ctgaatatat ttcaggcttc tccggactga
     5221 aggaagatat tcgccatcag ttactggatg agcagaaaat gacatcggat ggcatcactg
     5281 ccgattcttt actgaccatt tatcgtgagt tgtaccaccg ttttgaagtt ctgagaaaac
     5341 caagaaatat ccgtctgcta cccagccgct cggtaacaac tctggaaagt agtggtccgg
     5401 gctggaagtt attgatggaa catcatctgg atcggggcag agagagcctg gaaagtgatg
     5461 tggtgatttt cgccacaggt taccgttctg cattgccaca aatacttccc tcactgatgc
     5521 ccttgatcac catgcacgat aagaacacct ttaaagtgcg tgatgacttc actctggaat
     5581 ggagtggccc gaaagaaaat aacatctttg cggtcaacgc cagcatgcaa actcatggca
     5641 tcgccgaacc ccagctcagc ctgatggcct ggagatctgc acgtattctt aatcgcgtac
     5701 tggggcgtga tttattcgat ctcagtatgc cgcccgcact gattcagtgg cgcagcggca
     5761 gccggaaaaa accgcagccg gaggctgctg ctttaactca ctatacaaca aatattcagg
     5821 aataacgatg atgcgcaaaa agtatatgcc ccgggctctt ggtccgctgc ttcttgtcgt
     5881 gctgtcacca gctgtcgccc agcaaaacga tgataatgag atcatagtgt ctgccagccg
     5941 cagcaatcga actgtagcgg agatggcgca aaccacctgg gttatcgaaa atgccgaact
     6001 ggagcagcag attcagggcg gtaaagagct gaaagacgca ctggctcagt taatccccgg
     6061 ccttgatgtc agcagccaga gccgaaccaa ctacggtatg aacatgcgtg gccgcccgct
     6121 ggttgtcctg attgacggtg tgcgcctcaa ctcttcacgt tccgacagcc gacaactgga
     6181 ctctgtcgat ccttttaata tcgaccatat tgaagtgatc tccggcgcga cggccctgta
     6241 cggtggcggg agtaccggag ggttgatcaa catcgtgacc aaaaaaggcc agccggaaac
     6301 catgatggag tttgaggctg gcacaaaaag tggctttaac agcagtaaag atcacgatga
     6361 gcgcattgcc ggtgctgtct ccggcggaaa tgaccatatc tccggacgtc tttccgtggc
     6421 atatcagaaa tttggcggct ggtttgacgg taacggcgat gccaccctgc ttgataacac
     6481 ccagaccggc ctgcagcact ccaatcggct ggacatcatg ggaaccggta cgctgaacat
     6541 cgatgaatcc cggcagcttc aactgataac gcagtactat aaaagtcagg gggacgacaa
     6601 ttacgggctt aatctcggga aaggcttttc cgccatcagc gggagcagca caccatacgt
     6661 cagtaagggg ctgaattctg accgcattcc cggcactgag cggcatttga tcagcctgca
     6721 gtactctgac agtgatttcc tgagacagga actggtcggt caggtttact accgcgatga
     6781 gtcgttgcgg ttctacccgt tcccgacggt aaatgcgaat aaacaggcga cggctttctc
     6841 ctcgtcacag caggataccg accagtacgg catgaaactg actctgaaca gccaacttat
     6901 ggacggctgg caaatcacct gggggctgga tgctgagcat gagcgcttta cctccaacca
     6961 gatgttcttc gatctggctc aggcaagtgc ttccggaggg ctgaacaacc ataagattta
     7021 caccaccggg cgctatccgt catatgacat caccaatctg gcggccttcc tgcaatccag
     7081 ctatgacatt aatgatattt ttaccgtcag cggtggcgta cgctatcagt atactgagaa
     7141 cagggtagat gattttatcg actacacgca gcaacagaag attgctgccg ggaaggcgat
     7201 atctgccgac gccattcctg gtggttcggt agattacgat aactttctgt tcaatgctgg
     7261 tctgctgatg cacatcaccg aacgtcagca ggcatggttc aatttttccc agggggtggc
     7321 attgccggat ccggggaaat attatggtcg cggcatctat ggtgcagcag tgaacggcca
     7381 tcttcccctg acaaagagcg tgaacgtcag cgacagtaag ctggaaggcg tgaaagtcga
     7441 ttcttatgaa ctgggctggc gctttaccgg tgacaacctg cggactcaaa tcgcagcata
     7501 ttactcgctt tccaataaga gcgtggaaag gaataaagat ctgaccatca gtgtgaagga
     7561 cgacaggcgc cgtatttacg gcgtggaagg tgcggtggac tacctgatcc cggatactga
     7621 ctggagtacc ggtgtgaact tcaatgtgct gaaaaccgag tcgaaagtga acggtcaatg
     7681 gcaaaaatat gacgtgaagg aatcaagtcc atcgaaagcg acagcttaca ttagctgggc
     7741 gccggaaccg tggagtctgc gtgtgcagag caccacttct ttcgacgtaa gcgatgcaga
     7801 gggtaacgat attaatggtt acactaccgt cgattttatc agtagttggc tgcttccggt
     7861 gggaacactc agcttcagcg ttgagaacct cttcgaccgt gactatacca ctgtctgggg
     7921 acagcgtgca cctctgtact acagcccggg ttacggccct gcttcactgt acgactacaa
     7981 aggccggggc cgaacctttg gtctgaacta ctcagtgctg ttctgacggg gattcttttg
     8041 caacaagggt atgctgtaaa caacatggtc cttggtggcc atgttgttta cagccagtat
     8101 aatatacagc cttaacaaga tattccgtct ccggaatgga ctgttcacca ctgaataaca
     8161 atatgtctgt tttatggatc tttgactgaa atttatcgca tatcctcatt tcattgtccg
     8221 tctgaaaact aactgaaccg ccccgggttt cctggagagt attttatctg tgaactcagg
     8281 ctgccagatc attatttccg atggaagcat aataagcttt ttctgcttct gccggaggga
     8341 tatgacccag cctt
//
LOCUS       DAZUGK010000192         8333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZUGK010000192 DAZUGK010000000
VERSION     DAZUGK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.193"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..1211
                     /locus_tag="ACHY5W_003847"
     CDS             45..1211
                     /locus_tag="ACHY5W_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFS4448652.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1255..2571
                     /locus_tag="ACHY5W_003848"
     CDS             1255..2571
                     /locus_tag="ACHY5W_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFS4448653.1"
                     /translation="MTAIVNESVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHASMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2675..3325
                     /gene="fabR"
                     /locus_tag="ACHY5W_003849"
     CDS             2675..3325
                     /gene="fabR"
                     /locus_tag="ACHY5W_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFS4448654.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3325..3684
                     /gene="yijD"
                     /locus_tag="ACHY5W_003850"
     CDS             3325..3684
                     /gene="yijD"
                     /locus_tag="ACHY5W_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFS4448655.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3724..4824)
                     /gene="trmA"
                     /locus_tag="ACHY5W_003851"
     CDS             complement(3724..4824)
                     /gene="trmA"
                     /locus_tag="ACHY5W_003851"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFS4448656.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4938..5128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5193..7037
                     /gene="btuB"
                     /locus_tag="ACHY5W_003852"
     CDS             5193..7037
                     /gene="btuB"
                     /locus_tag="ACHY5W_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFS4448657.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAYTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYSALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6982..7839
                     /gene="murI"
                     /locus_tag="ACHY5W_003853"
     CDS             6982..7839
                     /gene="murI"
                     /locus_tag="ACHY5W_003853"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4448658.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            8213..>8333
                     /locus_tag="ACHY5W_003854"
     rRNA            8213..>8333
                     /locus_tag="ACHY5W_003854"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1926 a         1949 c         2267 g         2191 t
ORIGIN      
        1 aatccgttaa ttagcctgtt tataagtgaa aaaaggaata gccgatgatc gaacactata
       61 ttcgagggtt tgaggaagaa ctgcgtgaga tccgccatca gatccacgaa aacccggagc
      121 tgggattaca agaatttaaa accagtgctt tggttgcgga aaagctgcgt cagtggggct
      181 atgaagtaga gcaaggactg gcgacgacgg ggatagtggc cacgttaaaa gtgggcgacg
      241 gagagaaaag tattggcctg cgggcggata tggatgcgtt gccgatttat gaaaatagtg
      301 gcaagccctg ggccagtaaa catcctggtt taatgcacgc ctgcgggcat gacgggcata
      361 ccaccatact gctgggggcc gcgcgttatt ttgccgaaac ccgccgtttt aacggcacgt
      421 tacgtcttat cttccagcct gcggaagaga tgattaacgg cggtgagatc atggttaaag
      481 aggggctttt tgatcgtttc ccctgcgatg tcattttcgg catgcataac atgcccggtc
      541 tgccggtggg taagttttat ttccagccag gggcactgat ggcgtcaatg gatcagttcc
      601 atattacggt tcgtggttgt ggcggacacg gtgcgatccc gcacaaagcc atcgatccgg
      661 tgctggttgc cgcacatatc actaccgcat tacaaagcat tgtgtcgcgc aatgtcgatc
      721 cgctggaagc ggcggtaatt accgttggca gcattgttgc aggggaggcg gctaacgtca
      781 ttcctgacag tgctgagatg aaaatcagcg tccgctctct tagccgcgat acccgacaac
      841 ttcttctgac ccgtattcct gctttggcac aggcccaggc tgccagcttt ggtgctacgg
      901 ctgaggttac gcatgttaac ggtacaccgg tattagtgaa tgacgaggag atggcgcgct
      961 tcgcctggca ggtggcgtgt aaaacgtttg gtgaagatcg ggccgagttt ggcatcaagc
     1021 ccctgatggg cagtgaggat ttctctttca tgctggaagc ccaacccaaa ggcggtttcc
     1081 tgttattcgg taatggcgat gtcggggagg gttcctgcat ggtgcataac ccgggttacg
     1141 acttcaacga tgcaagtctg gtcccggcaa gcagctactg gggcgcactg gtagaagcct
     1201 ggctgcaata aataaaaaaa caacacaaca actctttatg gcaaggaata tggaatgacg
     1261 gcgatcgtaa atgaaagcgt gagtgtgaag aaaaagccta gtggcaggcg ggtgattttt
     1321 gcttcggcgt ttggcaatgc gctcgaattt tttgattttg gcgtttacaa cttttttgtg
     1381 gtctatatca gtacgctttt cttcccaccc agcgctgatc acaatgtcgc gctgttactg
     1441 gcatttgcaa cgtttggcgt gagttttttt atgcgcccgc ttggtggaat tatcgtcggg
     1501 gcctgggccg atcgtttcgg acgcaaaccc gcgatggtgt tcactattgc cctgatgagc
     1561 ctcggcacgt tgatgattgg cattgcgccg acgtatgcga cggcgggcta ttggggaacg
     1621 gcaactctgg tacttgctcg tttgattcag ggggttgcag caggcggtga agttggggct
     1681 tcaatgtcgc tactggtgga gtcagctccg gcaaatcgtc gtggctttta cagcagttgg
     1741 tcactggcga ctcaaggcct ggcaacaacc tttggtggtg ttgtcgcttt aggtttaagt
     1801 gcatggctgc cctttgcgac aggttcagaa accgtaatgg cagaatgggg ctggcgtgtg
     1861 ccgtttttta ttggcgtgtt attagcgcct gtcggctgtt ggttgcgttt gagtctggaa
     1921 aatgatgttc ctgagccagc acataataag aaggccgcag ccagcgaaag cgccttctcc
     1981 ttacttatgc aacataaagc gactatcgct aacggtatct tgctcgctat tggtagtaca
     2041 gtcgcaacct atatctccct cttttattac ggtacatggg cggcgaagta tttaggaatg
     2101 aaccaaaact attctcatgc ttcgatgtta ctggcgggcg ttattacgtt tgtgggtgcg
     2161 ctgctggtgg ggatgttgtg cgattcggtc gggcgtaaaa agctgatttt aatctcccgt
     2221 gtgatggtgc tgatctgtag ctggccgtca ttctggctgt tggtgaatta cccaagccct
     2281 ggcatgttgc tgacggtggt tttcgtgatg gtcagcttta ccacgcttgg cggtgtgcca
     2341 gtgatgttgt tgatctctga actgttgccg aaacggattc gggcattggg ttttgcgctg
     2401 gtttatagca ttggtgtggc aatctttggc ggttttgctc aatatttcgc tacgcaatcc
     2461 atcgtgttgc tggatagttt gacggctccg gcgtggtatc tgggtggagg cacgttgctg
     2521 tcgatgttgg cgttgttgta cgtaaaagaa ccggccaaag aattgcagta aatatgtttt
     2581 attgcgttac cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag
     2641 tgccagcagt acttttggca aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa
     2701 agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag
     2761 cttcgccagc ttgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt
     2821 ttatcggcat ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt
     2881 aatgctacgc caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat
     2941 ccgcacctcg gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt
     3001 attattgcgg gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat
     3061 tcagcacttc attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc
     3121 gtttactgaa gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc
     3181 gttggacgtc ggcgttgaac aacgtcggca attagaagag cgactggtac tgcaactgcg
     3241 aatgatttcg aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat
     3301 tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg
     3361 ctggcgttgg ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt
     3421 gtgccatttt ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa
     3481 cgttatctta atcgcaccat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt
     3541 ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac
     3601 ttcttcccgg cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac
     3661 cgtaagcagg aagttgctga gtaatcggta tcatgccgga tgctggcgca tccggcatgg
     3721 gttttacttc gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc
     3781 aaacagagcc agacgttcga ccttgtgcgt ctggcttaat gtttccagat ttttgcataa
     3841 cgtttccgga ttgcaggaga tatacagaat acgtggatac gcctgcacca ttttctcggt
     3901 ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt
     3961 taagtcgatc ccttgcaggc ggttaaactc gcgcacgcca ttcatcgcct gagtaaattc
     4021 ttctgccgcc atacgaataa tttgtacgtt atcaatatgg ttagcggcaa tattgtattg
     4081 cgcggcagcg actgacggct tggcgatttc ggtggctaat acccgatcaa aattgcgcgc
     4141 cagggctaat gagaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc
     4201 tttggttacg tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt
     4261 aaagctgttt tctacctgac ggtagatcat ctctttccct gcgaccggca gacgttcgtc
     4321 gatgtaatct tgatccagcg cgattttggt tttcgttgcc cgaccaatca gatgcacatt
     4381 cagattctgt gctcgcagtg catcgcgcag ggcttccgct tcctgacgcc actcatcatc
     4441 cagcttttta tggtacagca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc
     4501 aatctggaac aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt
     4561 catcaactgg ttgatgagtt cactggcggc ggggaagcta tccacgcgga tacggctttt
     4621 ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc
     4681 cgcacgcatc cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa
     4741 cggtgccatc atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc
     4801 tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt
     4861 agggattgct catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga
     4921 cagtaacttg ttacaacctg tagcatccac ttgccggtcc tgtgagttaa tagggaatcc
     4981 agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg
     5041 acactgccat tcggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg
     5101 aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg
     5161 cggcatcctt cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg
     5221 cgtgttctgt cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg
     5281 ttactgctaa ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga
     5341 cgcgtcagga tatcgaccgc tggcagtcga cctcggttaa tgatgtgctg cgccgtcttc
     5401 cgggcgtcga tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg
     5461 gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg
     5521 tgagtggttc tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata
     5581 tccgtgggcc acgctccgcc gtttatggtt ccgatgcaat aggtggggtg gtgaatatca
     5641 tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc
     5701 agaactatga tgtctctacg cagcaacaac taggggataa gacacgagta acgttgttgg
     5761 gcgattatgc ctatactcat ggttatgatg ttgttgccta tggtaatacc ggaacgcaag
     5821 cgcagccaga taacgatggt tttttaagta aaacgcttta tagcgcgctg gagcataact
     5881 ttactgatgc ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg
     5941 acgcgtatta ttctcccggt tcaccattgg tcgatacccg taaactctat agccaaagtt
     6001 gggacgccgg gctacgctat aacggcgaac tgattaaatc acaactcatt accagctata
     6061 gccatagcaa agattacaac tacgatcccc attatggtcg ttatgattcg tcggcgacgc
     6121 tcgatgagat gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta
     6181 gtattggtgc gggtgttgac tggcagaagc agagcacggc accgggcaca gcttatgtta
     6241 aggatggata tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg
     6301 attttacctt tgaaggcgca gcacgcagcg acgataactc acagtttggt cgtcatggaa
     6361 cctggcaaac cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg
     6421 ggacatctta taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc
     6481 tggaccctga gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga
     6541 actggcgtat ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca
     6601 ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt
     6661 ttgataccgg accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga
     6721 ttaccgacac gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc
     6781 agttgtatga cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg
     6841 attactcatc ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg
     6901 cggttgcgta tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg
     6961 acaaagatta tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg
     7021 gcagctacac cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg
     7081 ttgtcggtct atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc
     7141 gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg
     7201 gcaattgtca ccgcggtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac
     7261 accgccagta ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt
     7321 gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg
     7381 gcaacccgcg gaacagttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat
     7441 gaatgccaga tagaaatgct gggctcggca gagatggttg agttggctga agcgaagctg
     7501 catggcgaag atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg
     7561 aaagagccgc cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa
     7621 ctgttacaag tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc
     7681 cgaacggcct ggttgttaga acatgaagcc ccggatgcaa aatctgccga tgcgaatatt
     7741 gccttttgta tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac
     7801 ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt
     7861 cggaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
     7921 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaacttca
     7981 gcagagaaag caaaaacaaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc
     8041 gcgccgctga gaaaaagcga agcggcactg ctctttaaca atttatcaga caatctgtgt
     8101 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
     8161 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
     8221 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
     8281 gtaacaggaa gaagcttgct tctttgctga cgagtggcgg acgggtgagt aat
//
LOCUS       DAZUGK010000193         8288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZUGK010000193 DAZUGK010000000
VERSION     DAZUGK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.194"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..234
                     /locus_tag="ACHY5W_003855"
     CDS             <1..234
                     /locus_tag="ACHY5W_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448659.1"
                     /translation="VDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLT
                     PETLFGSKSQDKHALFPIFTASWRANRIKNKGI"
     gene            457..954
                     /gene="ubiC"
                     /locus_tag="ACHY5W_003856"
     CDS             457..954
                     /gene="ubiC"
                     /locus_tag="ACHY5W_003856"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFS4448660.1"
                     /translation="MSHPALTQLRALRYFTEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            967..1839
                     /gene="ubiA"
                     /locus_tag="ACHY5W_003857"
     CDS             967..1839
                     /gene="ubiA"
                     /locus_tag="ACHY5W_003857"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFS4448661.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHY5W_003858"
     CDS             complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHY5W_003858"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFS4448662.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHY5W_003859"
     CDS             4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHY5W_003859"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4448663.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5066..5674
                     /gene="lexA"
                     /locus_tag="ACHY5W_003860"
     CDS             5066..5674
                     /gene="lexA"
                     /locus_tag="ACHY5W_003860"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4448664.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5747..7072
                     /gene="dinF"
                     /locus_tag="ACHY5W_003861"
     CDS             5747..7072
                     /gene="dinF"
                     /locus_tag="ACHY5W_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFS4448665.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFIAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLATIWRRHWRNGTWFAAT"
     gene            7188..7397
                     /locus_tag="ACHY5W_003862"
     CDS             7188..7397
                     /locus_tag="ACHY5W_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4448666.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHY5W_003863"
     CDS             complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHY5W_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFS4448667.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1879 a         2020 c         2276 g         2113 t
ORIGIN      
        1 gtcgatgatg ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct
       61 acaaaagata ccaattggaa ttccacgtat atctacaaat ccagacatga gatgttgcct
      121 gttaatctta ctccggaaac acttttcggc tccaaatctc aggataaaca tgcgcttttc
      181 ccaattttta ctgcgagttg gcgagctaat cgtataaaga ataaaggtat ttaagtaaag
      241 gaaaacatca ccgttcctgg catcctgggc ggtgatgccc ctatggttgc cctcgccagc
      301 acgggcatcg gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt
      361 ttttgttgcg cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga
      421 tacaatgcct ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa
      481 ctgcgtgcgc tgcgctattt tacagagatc cctgccctgg atccgcaact gctcgactgg
      541 ctgttgctgg aagattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagcgtg
      601 acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg
      661 ccgaaagagt ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg
      721 cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa
      781 aaattgggta aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac
      841 tttattgaga taggccgtga tgccggactg tgggggcgac gttcccgtct gcgattaagc
      901 ggtaaaccgc tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa
      961 aaaaatatgg agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt
     1021 acggataagc caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg
     1081 gcgacaccgg gcgttcccca gctctggatc ctggcggtgt ttgtcgcggg tgtctggctg
     1141 atgcgcgctg ctggttgcgt ggtgaatgat tatgctgacc gcaagtttga tggtcatgtt
     1201 aagcgcacgg cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg
     1261 ctgtttgtag tgctggtact gatttcgttt ttactggtgc tgacgctgaa tacgatgacc
     1321 attctgttgt cgattgccgc gctagcgctg gcgtgggtgt acccgtttat gaagcggtat
     1381 acccatctac cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt
     1441 gctgctgtga gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc
     1501 tgggcggtgg cttacgacac gcagtatgcg atggttgacc gtgatgatga tgtgaagatt
     1561 ggcattaaat ccaccgcaat cctgttcggc caatacgata aattgattat tggtattttg
     1621 cagattggcg tactggcgct gatggcgatt atcggtgagt taaatggctt aggctgggga
     1681 tattactggt caattctggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc
     1741 aaccgcgagc gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta
     1801 ctgtttttag ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga
     1861 tcatccggct ttcttctggg ctgcctgatg cgctgcgctt ctcaggccta cacaacagat
     1921 cgcaatttat tgaatttgca gatcatggaa ggccggataa ggcgtttgcg ccgcatccgg
     1981 caattctcac tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg
     2041 atgtaatcaa ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc
     2101 cgctatcgct gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct
     2161 tgtcgaagaa ctccggcgcg ttgataccgt gcagcacgga gagacgttgc gcgacggtgc
     2221 ggctctcttt ctccagcgta ccgcggttga tcgacgggtt ggcgctcaac agccagaagg
     2281 tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc gagcagctgt agcgtgcgag
     2341 aatgcgccgg gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca
     2401 tctcatttgc cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca
     2461 gctccgcttt cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga
     2521 tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa
     2581 tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg cggcagaatg atgatgtcgc
     2641 cgatagtgtc tttctcaact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc
     2701 tggcgctggc tgaaggaacg gtagagtccg tggagtaagg cacgttgcgc atcagatcca
     2761 ggtagcagtt gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgacgcca
     2821 gtagcgcggt acagcagagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca
     2881 tcagatcggc agcaatatta ttgaccgtcg gtgttaacca ggccggacgc accgcttcga
     2941 tgggatcgat agattcacgc cagtctggta catgctggtt aaggtaggtc atcaacggca
     3001 ttggttcacc gaagttgacg taaccctggc cgagattacg cagcttgctt aaaccgcgca
     3061 gcatctgcgg caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac
     3121 ccacttccat gacgtgctca taaccgatgt agatcggaat cagcgtaatc ggacgcgtgc
     3181 cgccacgcag catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac
     3241 gccccgtacg ggaacgaccg ccttctacga agtactcgac ggaataacca cggctgaaca
     3301 gttcgccgag atactcacgg aaaacggtgg aatagagctt attgccttta aaagtacggc
     3361 gaataaagaa cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga
     3421 tcccggcggc gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagcagca
     3481 ggtagtccat gtgactgcgg tggcaaggca catataccag ctcatgaccg tcgtgggcca
     3541 gctggcgaac gcgctcggcg ttatggacgt tgataccctg gtaaaggcgg ttccaggtaa
     3601 agcccagaat acggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt
     3661 ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt
     3721 catcttctac cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac
     3781 gagcaggcag acgtggaccc acggcagcca gacgttgacg ggcaaagtgc atacgcgcca
     3841 cgcgcgccag tttctgcgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca
     3901 gcgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa
     3961 atttctgtac gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccctg
     4021 gcgcgcgacc aaacatcacc gatactggca ccatctgcac atccagattt gggttgctac
     4081 ggtgcaaatc gagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg
     4141 tgaatacacg cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt
     4201 ccagcggctc taacgggtca ggcaggtcat gtgccagaca ctgggcgcgc aacgtcagca
     4261 agtctgcttt cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca
     4321 gttccggggc aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat
     4381 tcagtaattt gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta
     4441 ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc
     4501 atgtttcgcg cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga
     4561 cgctcataat gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaatt
     4621 atcaaagctg ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca
     4681 ttccgtcagg aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg
     4741 gacgcgatta cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc
     4801 ctcaatagcg ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc
     4861 ggacgcgcta aagatatggg atccgccgcg gtgctgattg cgattatcgt cgccgtgatt
     4921 acctggtgca tcctgttatg gtcgcatttt ggataaccct tccagaattc gataaatctc
     4981 tggtttattg tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata
     5041 actgtatata cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt
     5101 tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc
     5161 gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg
     5221 caaaggcgta attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga
     5281 ggaagaaggg ttgccactgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca
     5341 gcatattgaa ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct
     5401 gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc
     5461 agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga
     5521 agttaccgtt aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag
     5581 cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc
     5641 ggttggcgtt attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc
     5701 tggcgtcgcg gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac
     5761 ttcatctgat aaagcacttt ggcatctcgc cttacccatg attttctcca atatcaccgt
     5821 tccgttgctg ggactggtcg atacggcggt aattggtcat cttgatagcc cggtttattt
     5881 gggcggcgtg gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt
     5941 acgcatgagc accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt
     6001 agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct
     6061 gctgcgtaca ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct
     6121 ggaacaggcg cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa
     6181 tctggtatta ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt
     6241 agtggtcggc aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctacatat
     6301 gaacgtgcag ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg
     6361 tctgctaatg gtgcgtaaaa tcctcaaact acgcggaatc tccggcgaaa tgctgaaaac
     6421 tgcctggcga ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc
     6481 gctgttgttg caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga
     6541 cattatcgct gttaacgcgg ttctgatgac gctactcacc tttatcgcct atgcgctgga
     6601 tggttttgcc tacgcggttg aagcgcattc cggtcaggcg tacggtgcac gcgacggtag
     6661 ccagttgctg gatgtctggc gggcagcgtg ccgccagtcg gggatcgtag cgttactgtt
     6721 ttcggtggtt tatttgctgg caggggaaca tatcattgcg ttgctgacgt cgttaaccca
     6781 gattcagcag ctagccgacc gctatcttat ctggcaggtg attttgccgc tggtgggcgt
     6841 ctggtgttat ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa
     6901 cagtatggcg gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg
     6961 taatcatgct ttgtggctgg cgttaaccgt ctttctggcg ttgcgcgggc tttctctggc
     7021 gactatctgg cggcgtcact ggcgcaatgg tacctggttt gccgcaacgt gacggttaaa
     7081 aattctgaat aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag
     7141 aaaagtgaac gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg
     7201 aagccggcgg taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga
     7261 ccgatgatga tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg
     7321 aacgttatgg ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca
     7381 atgaatatcg ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt
     7441 aacgcggttt ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac
     7501 acgcttccac ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca
     7561 gggcaaaccc catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacatt
     7621 cttctttcac tgcgccgcag cgatcgcaaa taaacatggc tgacgtatgg gtgggctgat
     7681 cgaacagatg acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct
     7741 caagcagaaa atccagcgcg cgataaaccg ttggcggctt ggcctgcggt tcagcttcgc
     7801 gcagcaaatc aagcagatcg taagcgctga tagcgccatc ctgcagactc atcaggcgca
     7861 acacttccag gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct
     7921 gcgctaataa ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga
     7981 atgtccttac tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg
     8041 ttgattttct gttttgtagg taaggtgttg tgttgcctgg tcgtaccatt atcaacacga
     8101 taataattaa taattcattt ttaaatgaat tgctttaagt taactcacta tttatttaac
     8161 tgggtttgat cgcatgcctg tagtccagaa attatgagcg atttttgata gtgaccagat
     8221 ggcaaaagaa ttataaggag gacacggtac gacaaaaaag gtagatgaat catgttgaag
     8281 ataatacc
//
LOCUS       DAZUGK010000194         8261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZUGK010000194 DAZUGK010000000
VERSION     DAZUGK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.195"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(52..357)
                     /locus_tag="ACHY5W_003864"
                     /pseudo
     CDS             complement(52..357)
                     /locus_tag="ACHY5W_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
     gene            complement(404..652)
                     /locus_tag="ACHY5W_003865"
     CDS             complement(404..652)
                     /locus_tag="ACHY5W_003865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448668.1"
                     /translation="MSPANKFMFAGMLMMMGAVLLLELYDAKTDKETCDCQLCITLQI
                     YVASNIKIGRNDGAYILWRLDNHLVIQNNTVLYIYLVL"
     gene            775..1464
                     /gene="paoA"
                     /locus_tag="ACHY5W_003866"
     CDS             775..1464
                     /gene="paoA"
                     /locus_tag="ACHY5W_003866"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFS4448669.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNPHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1461..2416
                     /gene="paoB"
                     /locus_tag="ACHY5W_003867"
                     /pseudo
     CDS             1461..2416
                     /gene="paoB"
                     /locus_tag="ACHY5W_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
     gene            2413..4611
                     /gene="paoC"
                     /locus_tag="ACHY5W_003868"
     CDS             2413..4611
                     /gene="paoC"
                     /locus_tag="ACHY5W_003868"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFS4448670.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWFEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYYQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLAALDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQKSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            4621..5572
                     /gene="paoD"
                     /locus_tag="ACHY5W_003869"
                     /pseudo
     CDS             4621..5572
                     /gene="paoD"
                     /locus_tag="ACHY5W_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
     gene            complement(5751..6878)
                     /locus_tag="ACHY5W_003870"
     CDS             complement(5751..6878)
                     /locus_tag="ACHY5W_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4448671.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWLCISLPSMDANKSHASFGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(7020..8078)
                     /locus_tag="ACHY5W_003871"
     CDS             complement(7020..8078)
                     /locus_tag="ACHY5W_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4448672.1"
                     /translation="MSYADPTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSNDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
BASE COUNT         1935 a         2373 c         2263 g         1690 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct caacccagtt
       61 aaagacatgc ccggcaaagg tatatacagc cgcattggga tctgtcagcc ccagccaatc
      121 tcgaagaaaa atatacggag gattgagaaa attacgcgag caatcaattt gcccagcagc
      181 cctgattaat gattccgggc cacacgctgc attaaacata tccaccgggc tacgtggcgg
      241 caatggaact tcagcccccc acttcacaaa tgcggaaaca acgccagcaa tcagcccaat
      301 gaatgcagca agaccataac gtctgcggtt cggtggagtt tgttcaaata tattcatatc
      361 taccctgctt gtaccattat gttatacacc tctttaggtg tattcataaa acaaggtaaa
      421 tgtaaagaac tgtattgttt tgtataacaa gatggttgtc taatcgccaa agaatataag
      481 caccatcatt tctccctatt tttatattag aagcgacata gatttgcagg gtgatgcaga
      541 gctgacaatc acaggtttcc ttatcggttt ttgcatcgta caactcaagc aataaaacag
      601 ctcccatcat catcaacatc ccggcgaaca tgaatttatt agccgggctc atagctgcat
      661 tcgcgtttta agaataatcc tcctgctgtc gccgactatg cttaacgttt aaaaaagcat
      721 cagcactctc gcaacgcact cttattttcc cctttagaat accggaggcc tggtatgagc
      781 aaccaaggcg aataccccga agataatcgg gttgggaagc atgagccgca cgatttaagt
      841 ttgacccgtc gcgatctgat taaagtgagc gccgcaacgg cggcggccgc cgtggtttat
      901 cctcattcta cgcttgcggc aagcgttccg gcagctacac ccgcgcctga gataatgccc
      961 ctgacactta aggtgaacgg caaaaccgag cagcttgagg tggatacccg aaccacgcta
     1021 ctggacgctt tgcgtgaaaa tctgcatttg atcggtacca agaaaggttg cgatcacgga
     1081 cagtgcggag cctgtaccgt gctggtcaat ggtcgcaggc ttaatgcctg cctgacgctt
     1141 gcagtcatgc atcagggggc cgagatcacc accattgaag gcctgggttc accagataat
     1201 cctcacccca tgcaggcggc ctttatcaag catgatggct tccagtgcgg ctactgcacc
     1261 tccgggcaaa tttgctcatc agtagcggtg ctaaaagaga ttcaggacgg cattcccagt
     1321 cacgtcacgg tcgatttggt ttcccctcca gaaagaactg ccgatgagat ccgtgaacgt
     1381 atgagcggca acatctgtcg ctgtggtgca tacgctaaca tccttgccgc cattgaagac
     1441 gctgcggggg agataaaatc atgaaggcgt ttacctatga acgagtgaac acccccgccg
     1501 aggcggcact tagcgctcag cgcgtacccg gcgcaaaatt tatcgcgggc gggaccaatc
     1561 tgctggacct gatgaagctg gaaattgaaa cgcccaccca ccttatcgat gtgaacgggc
     1621 tcgggctcga taagatcgaa gtgaccgacg cgggtgggct gcgcatcggc gcactggtac
     1681 ggaacaccga cctggcggct cacgagcgcg tgcgtcgtga ttacgcggta ctctcccgcg
     1741 ccctgctcgc tggcgcgtct ggtcagttac gcaatcaggc aaccacagca ggtaatctgc
     1801 tccagcgcac gcgctgcccc tatttttacg acaccaatca gccctgtaat aagcgcctgc
     1861 ccgggagcgg ctgcgcggcg cttgaaggct ttagccgtca gcacgcggtg gtaggcgtaa
     1921 gcgaagcctg cattgccacc catccgagcg atatggcggt cgcaatgcgg ttgctggatg
     1981 cggtggtgga aaccatcacg ccggagggaa agactcgcag tatcacactg gctgattttt
     2041 atcacccacc ggggaaaacg ccgcacattg aaaccgccct gcttcccggt gagcttatcg
     2101 ttgcggtgac gttacctccg ccgctcggcg gaaaacatat ttaccgtaag gtgcgcgatc
     2161 gcgcctccta cgcctttgcc ctggtatcgg tcgcggcgat tattcagcct gacggcagcg
     2221 ggcgcgtcgc gctgggtgga gtagcacata agccctggcg cattgaggct gcggatgctc
     2281 agctatccca ggggcgcagg ccgtatatga cgcgctgttc gccagcgccc atcccaccgc
     2341 tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac tggctgaagc
     2401 gagggcacaa gcatgaaatt tgataaaccc gcaggggaaa acccgatcga tcagctgaag
     2461 gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg cacggcacgc
     2521 tacgcctacg aatggtttga agaagctccc aacgccgcct atggctatat cgtcggttcc
     2581 gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa agcgccgggc
     2641 gtactggctg tcattaccgc cagtaacgcc ggggcactcg gcaaaggcga caaaaacacc
     2701 gccaggctgt taggcggccc cactattgag cactattatc aggccattgc gctggtagtg
     2761 gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcgca ctatcgccgt
     2821 aataaaggag cttactccct ggcggacgaa aaacaggccg tcagtcagcc gccggaagac
     2881 acgcccgaca aaaacgtcgg tgactttgac ggggctttct cctccgctgc ggtgaagatt
     2941 gatgctacct acacgacccc ggatcagagc catatggcga tggagccgca tgcctcgatg
     3001 gccgtctggg atggaaataa gcttactctc tggacctcaa atcagatgat tgactggtgc
     3061 cgcaccgatc tggcaaaaac gctaaaagtg cccgtggaga atgtgcgtat tatctccccg
     3121 tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct gctggcggcc
     3181 ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc cactattccc
     3241 aataacacca cgcaccgccc cgcaaccctt cagcatctgc gtatcggtgc tgaccagagc
     3301 gggaaaatca ccgctatctc acatgaaagc tggtccggaa acctgcccgg cggcacgccg
     3361 gaaacggcgg tacagcaaag cgaattactc tacgcagggg caaaccgtca taccggcctg
     3421 cggctcgccg cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc
     3481 ggtctgatgg cgctcgaaat cgcgatcgat gaactagcgg aaaaagcggg catcgatccc
     3541 gtcgagtttc gcatcctgaa tgacactcag gttgaccccg ccgacccgac gcgccgcttc
     3601 tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg ctggaagcag
     3661 cgcaacgcca cacccggaca ggtacgcgac ggagagtggc tagtcggcca cggcgtcgcg
     3721 gcgggctttc gcaataatct gctggaaaaa tcgggtgcgc gggttcacct cgaacaaaac
     3781 ggcaccgtta ccgtggaaac ggacatgacc gacattggca ctggcagcta caccattctg
     3841 gcccagacgg cggcggaaat gcttggcgta ccgctggagc aggttgcggt tcacctcggc
     3901 gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa tacctccacc
     3961 tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc agtcgggttt
     4021 gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg aagcgccacg
     4081 ctacatgagg ccaccgcagg cggcagactg acagcggaag agagtattga attcggaaca
     4141 ctgagcaagg agtaccagaa gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat
     4201 agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc
     4261 ctgaatccga aaactgcacg cagccaggtc attggcgcaa tgactatggg catgggcgcg
     4321 gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa tcacgatatg
     4381 gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaggtgat tttcctggat
     4441 gataccgacc ccatatcctc cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc
     4501 ggcgtgagcg ctgctatcgc caacgcggtg tataacgcca ccggtattcg ggtacgcgat
     4561 tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta aggaggaaca
     4621 atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca ggcgtttctc
     4681 accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga
     4741 gcggtgctgg tgacgctggt ggagatacac ggcggcgcgc ccgctcgggg cgcagatggt
     4801 ggttcgcgaa gatggtcgtt actgcggttt tgtctctggc ggctgcgtgg aggccgctgc
     4861 cgcttttgaa gcgctggaga tgatgggctc aggccgcgat cgcgaaattc gctatggcga
     4921 aggttcgccg tggtttgaca tcgttctgcc ctgcggcggt gggatcacgc tgacgctcca
     4981 taaactacgc tcggcacagc ctctgctcgc cgtgctgaac cgcctggaac agagaaaacc
     5041 ggcgggtctg cgctacgatc cgcaagcaca atcgctggtg tgcctgccca cgcaaacccg
     5101 gacgggctgg aatctcaatg gctttgaggt ggggttcagg ccatgcgtca ggctgatgat
     5161 ttacggacgt tctcttgagg cgcaggcaac cgcgagtctt gcagcagcca caggctatga
     5221 cagccatatc ttcgatcttt ttccggcctc agccagcgct cagatcgata ccgatacggc
     5281 ggtcattttg ctgtgccatg atctcaaccg ggagctgcca gtgttgcagg ccgcgcgaga
     5341 ggcaaaaccc ttttatctcg gcgcattggg cagctatcga acccacactt tacgtctgca
     5401 aaagctccac gagctgggat ggtccaggga ggagacagcg caaatccggg cacccgtcgg
     5461 gatattcccc aaagcccggg atgcgcatac tctggcactc tccgtgctgg cagaagtcgc
     5521 ctctgtacgt ctccatcagg aggaggattc atgcctgccc ccgtcgtcct gatccttgcg
     5581 gccgggcgtg gaaagcgctt tctcacctgc gggggaaata ccataaatta agagaaaaat
     5641 ctgcagtagc cgttttagaa aatcacctaa atcgtttaaa agagcgccct acccatagtg
     5701 gttgctcatc gccttgtgta acttattcag caacgcgcgc cctcgccgga ctaaagcgca
     5761 ccggccttct ggcgtgcggt aacaaaggtc agtacgcccg cgcaaaggag cagcacacta
     5821 ctcatggcaa acgtactctg ccagccaagg cggtcaaaca cgatgcctcc tgccgttgat
     5881 ccaagggcga ttgagagttg aatcaccgcg accatgagcc cgcctccggc ttcggcgttg
     5941 tcaggcaacg tacgcgcaat ccatgtccac catccggttg gcgcggcggt ggccagcatg
     6001 ccccagagtc ctaacagcag cgagaccgtc cagacgtgat gcccggtaag catcagaccg
     6061 atggcaatga cggccatcag tccgggtatg gccatcagcg tggggtagaa ttttctgttg
     6121 agaaatgtcg aaacaaccag cgtgccaata aaaccggcca cgccgataat cagcaaaatc
     6181 aacgacaagc ctaaggaatt aacccgtgtc accgactcca gaaacgggcg cacataggtg
     6241 aatagcgcaa actgccccat gaaaaacagg ccacaagcta tcatgccgac cacgaccatg
     6301 cggcggctaa ataagcggaa taccgcgcca aatgacgcgt ggcttttatt cgcatccatg
     6361 ctgggcagac taatacacag ccagataaag gcgacaaccg cgataggcac caggcacaag
     6421 aaagcgcctc gccatccgac cgtagccccc agatagcttc ccagcggtgc ggccacgacc
     6481 gttgccagcg cattaccggc gttgaaaatc gccagcgcac gcgtgacctg atgctgcggc
     6541 accagacgaa tcgccgttgc cgctgacatc gaccagaatc cgccaatggc cacaccaatc
     6601 atcgcacgac cgaccatata catcagataa ctggtagcca gcgcaataat aagccccgat
     6661 acggccatca gaaccgtcat ccccagcagc aggaacttac gattcatctt tccagctagc
     6721 gttgaaagcg tcaggctggt cagaaccgcc agcgcgccgg agatagcaat cccctgccct
     6781 gccagcccct ccgtcacgcc taaatcgcgg gtaatcggcg tgagcaggct gacgggcata
     6841 aattcagaag caatcagcac aaacacgcac agggtcatgg caaaaatacc gccccagtat
     6901 gcgcgctgat gatgaggtga tagtgtttgg cttagtgtcg acatagtgaa atttcacaaa
     6961 gatcaggata aaagccaccg gccatagacc ggtggctttg catcaattca ggagaggttt
     7021 tatttcagtt tggtctggaa aaattgttcg aacttcgcga aaggtatttt tccggccacg
     7081 ttatcgtaca ggtccacgtg atttgcccct ggtaccacca ccagctcttt gtcgttactg
     7141 ccaaccgcct tgtaagcatc ttcagcaaaa tagcgtgaat gcgctttctc accggtcaca
     7201 atcagcgtcg ggatggtgat ttcactggcg tagctcagca gcggcatatt cataaatgac
     7261 agcggcatcg ttgccgtcca cgccccggta gagttaacgg aacgttcgtg gaagccgcgc
     7321 ggcatgcgat agtagtcaaa gaactctttc agtaccggat gaggatttgc cggcagcgtt
     7381 tctggcagaa tgcgctcgcc cgcactgacc ttgccgttgc tatcgacatt aatatcatga
     7441 ccaccgtgag cgaacgttcc gctctgcgca tccttccagc gctgttcatt cagatattgc
     7501 agaacagcac gacggtcggc ggtagaataa cggtcttcgc catcccccac accatggccc
     7561 atcgcccggc tcatgtcata catcacgctg gtagcaaccg ctttaacgcg ggtatccatc
     7621 gccgcgtcgt tcagcgccat gccgccccag ccacaaatac cgagcagccc gatacggttg
     7681 cgatctacct ctttttgcag ccctaagaaa tccaccgccg cgctgaaatc ttcagtgttg
     7741 atatccggtg aggcgacgtt tcgcggatag ccgccgcttt ccccggtgta agaaggatcg
     7801 aacgccaggg taacaaaccc tcgttccgcc agcgtctgcg catacaagcc gctggattgc
     7861 tctttcaccg cgccaaatgg cccactgacc gcaatcgccg ccagcttgcg atcgccacgg
     7921 tctttaggca ggtaaagatc gcccactaag gtgatgccat atcggttcgg gaacgagact
     7981 ttacggtgat cgactttctg gctctcggcg aacgttttat cccatttatc tgtcatggac
     8041 acaggggcat taggattggt tggatcagca taactcattg tcgtgactcc acttaatgat
     8101 gcgaaaagca gcatggccgg catcgcggtt tttagcattc gggtgaaggc tttcataaaa
     8161 atccccataa aaaacaacag agagatacag gtttccgacg gacaacgctg cggtgcttta
     8221 ctttgatggg gtcattatag tgagcagaat tagtgctgat t
//
LOCUS       DAZUGK010000195         8155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZUGK010000195 DAZUGK010000000
VERSION     DAZUGK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.196"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            57..413
                     /gene="ydiL"
                     /locus_tag="ACHY5W_003872"
     CDS             57..413
                     /gene="ydiL"
                     /locus_tag="ACHY5W_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFS4448673.1"
                     /translation="MNAYELQALRHIFAMTIEECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDCNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            513..1727
                     /gene="ydiM"
                     /locus_tag="ACHY5W_003873"
     CDS             513..1727
                     /gene="ydiM"
                     /locus_tag="ACHY5W_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4448674.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYIFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            1954..3219
                     /gene="ydiN"
                     /locus_tag="ACHY5W_003874"
     CDS             1954..3219
                     /gene="ydiN"
                     /locus_tag="ACHY5W_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4448675.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            3231..4097
                     /gene="ydiB"
                     /locus_tag="ACHY5W_003875"
     CDS             3231..4097
                     /gene="ydiB"
                     /locus_tag="ACHY5W_003875"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFS4448676.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLRQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            4128..4886
                     /gene="aroD"
                     /locus_tag="ACHY5W_003876"
     CDS             4128..4886
                     /gene="aroD"
                     /locus_tag="ACHY5W_003876"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4448677.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            5032..6627
                     /gene="ydiF"
                     /locus_tag="ACHY5W_003877"
     CDS             5032..6627
                     /gene="ydiF"
                     /locus_tag="ACHY5W_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFS4448678.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            6641..7791
                     /gene="ydiO"
                     /locus_tag="ACHY5W_003878"
                     /pseudo
     CDS             6641..7791
                     /gene="ydiO"
                     /locus_tag="ACHY5W_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(7834..>8155)
                     /locus_tag="ACHY5W_003879"
     CDS             complement(7834..>8155)
                     /locus_tag="ACHY5W_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4448679.1"
                     /translation="TLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWLL
                     TNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPV
                     SLVG"
BASE COUNT         2118 a         1851 c         1951 g         2235 t
ORIGIN      
        1 agcaagctga taatgttatt tatattttat tgtttatttc atgcagccat tacacgatga
       61 acgcttatga actccaggca ctccgccata tttttgccat gactattgaa gaatgcgcga
      121 cctggattgc ccagaccggt gacagtgaaa gctggcgtca atgggaaaat ggcaagtgtg
      181 ccattcctga ttgtgtagtc gagcaactgt tggctatgcg tcagcaaaga aaaaaacatc
      241 ttcatgccat catagagaaa ataaataacc gcatcggcaa caacaccatg cgttttttcc
      301 ccgacttaac cgcatttcaa cgagtctacc ctgattgcaa ttttattgac tggaagatct
      361 atcaatcggt agccgctgaa ttatatgcac acgatctgga gcggctttgt taatttttcc
      421 acagaaagga attgtcgttg ttacaacaat aatgaatgga tactgacaca acatcgctac
      481 actttttaaa gcacctttgc taagtataac ctatgaaaaa tccctattac cctaccgcac
      541 ttgggttgta ttttaattac ctagtgcatg gtatgggcgt cattttgatg agcctgaata
      601 tggcctcgct ggagacactt tggcagacta atgccgcggg tgtctcgata gttatctcat
      661 cgctgggcat tggtcgatta agtgtcttgc tttttgcagg attattatcc gatcgctttg
      721 gtcgccgccc ttttatcatg ctcgggatgt gctgctatat ggccttcttt tttggcatcc
      781 tacacaccaa taacatcatt atcgcttata tttttggctt tctggcggga atggcaaaca
      841 gttttctcga tgcaggcact tatcccagtt tgatggaagc ttttccacgc tcacctggga
      901 cagccaatat tttaattaaa gcatttgttt ccagcggaca atttttatta ccgctaatca
      961 ttagcctgtt agtgtgggct gaactgtggt tcggttggtc ctttatgatt gctgcaggca
     1021 ttatgtttat taacgctctt tttttatacc gttgtacgtt cccaccccat ccgggtcgtc
     1081 gcttacctgt cataaagaaa accaccagct ctacggaaca tcgctgttca attatcgatt
     1141 tagccagtta taccttatat ggctatatct caatggcaac gttttatctg gttagccagt
     1201 ggctggcaca gtacggacaa tttgttgcag gcatgtcgta cactatgtcg atcaaactac
     1261 tcagtatcta caccgtgggt tcgctgcttt gtgtatttat taccgctcca ctcattcgta
     1321 ataccgttcg cccaacaaca ttactgatgc tgtacacctt tatctcattt attgctctgt
     1381 ttaccgtctg cctgcatccc acattttatg tggtgataat atttgctttt gtcattggtt
     1441 ttacctctgc tggaggtgtt gtgcaaattg gcctgacgtt aatggctgaa cgtttccctt
     1501 acgctaaagg taaagctaca gggatctatt acagtgcggg cagtattgcg acctttacta
     1561 ttccgttgat tacggctcat ctgtcccaaa gaagtattgc cgatattatg tggttcgata
     1621 ccgccatcgc tgccatcggt tttttactgg cactgtttat cggcttacgc agccgcaaaa
     1681 aaacgcggca tcactcgcta aaggaaaatg tcgctccggg tgggtaatgc aatattcttt
     1741 tcaggtcatg caagatctta cggataaata actctttctg cgctaactaa ggaaaatcgc
     1801 gatcaaaaac aaactatgac atgcaatatt cctggaaaca taaactttat gccatgtacc
     1861 cagggaaaat catcttcagt atagtaatta tgtaaaccgt cggagaacaa tacgtacggt
     1921 aacgaaatta tctttcagca aggagctgtg aaaatgtctc aaaataaggc tttcagcacg
     1981 ccatttatcc tggctgttct ttgtatttac ttcagctact tcctgcacgg cattagtgtt
     2041 attacgcttg cccaaaatat gtcatctctg gcggaaaagt tttccactga caacgcgggc
     2101 attgcctact taatttccgg tatcggtttg gggcgattga tcagtatttt attcttcggt
     2161 gtgatctccg ataagtttgg tcgtcgggcg gtgatattaa tggcagtaat aatgtatctg
     2221 ctattcttct ttggtattcc cgcttgcccg aatttaactc tcgcctacgg tctggcagtg
     2281 tgcgtaggta tcgctaactc agcgctggat acgggtggct accccgcgct catggaatgc
     2341 tttccgaaag cctctggttc ggcggtcata ctggttaaag cgatggtgtc attcgggcaa
     2401 atgttctacc caatgctggt gagctatatg ttgctcaata atatctggta cggctatggg
     2461 ctgattattc cgggtattct gtttgtactg atcacgctga tgctgttgaa aagcaaattc
     2521 cccagccagt tggtggacgc cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg
     2581 ttagtctggc tggaaggtgt ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt
     2641 tatgtgattg tggtgtggat gcccaaatat gcgatggctt ttgctggtat gtcagaagct
     2701 gaggcattaa aaaccatctc ttattacagt atgggctcgt tggtctgtgt ctttattttt
     2761 gccgcactac tgaaaaaaat ggtccggccc atctgggcta atgtatttaa ctctgcactg
     2821 gcaacaataa cagcagccat tatctacctg tacccttctc cactggtgtg taatgccggc
     2881 gcctttgtta tcggtttctc agcagctggc ggcattttac agctcggtgt ttcggtcatg
     2941 tcagagtttt tcccaaaaag caaagccaaa gtcaccagta tttatatgat gatgggtgga
     3001 ctggctaact ttgttattcc gctgattacc ggttatctgt cgaacatcgg cctgcaatat
     3061 atcattgttc tcgattttac tttcgcgctg ctggccctga ttaccgcaat tattgttttt
     3121 atccgctatt accgcgtttt tattattcct gagaatgatg tgcggtttgg cgagcgcaaa
     3181 tttagcaccc ggttaaacac aattaagcat agaggttaaa ggagttaatt atggatgtta
     3241 ccgcaaaata cgaattgatt gggttgatgg cttatcctat ccgccacagt ttatcgcctg
     3301 aaatgcagaa taaagcctta gaaaaagcgg gattgccatt tacctatatg gccttcgaag
     3361 tggataacga tagctttccc ggagcaattg aaggattaaa agctctaaaa atgcgcggaa
     3421 ctggcgtatc aatgccgaac aaacaactgg cgtgtgaata tgttgatgaa ttaacgccag
     3481 cagccaaact ggtgggtgcc atcaacacca tcgttaatga tgatggctat ctgcgtggct
     3541 ataacaccga cggcacgggc catattcgcg ccattaaaga gagcggtttt gatatcaaag
     3601 gcaaaacgat ggtgctgtta ggggccggag gtgcctcaac ggcgattggc gcgcaggggg
     3661 caattgaagg tttaaaagaa attaaactct ttaaccgtcg ggatgagttc ttcgataaag
     3721 ccctcgcctt cgcgcagcgg gttaacgaaa acaccgattg tgtcgtcacg gttaccgatc
     3781 tcgccgatca gcaagccttt gctgaagccc tggcttccgc cgacatttta accaatggca
     3841 caaaagtggg tatgaaaccc cttgagaatg aatcattagt taatgatatc agtctgttac
     3901 atccgggact gctggtcact gaatgcgtgt ataacccgca tatgacgaag ttattgcggc
     3961 aggcgcaaca agctggttgc aaaacgattg atggatacgg catgttgttg tggcaagggg
     4021 ctgaacagtt cacgttatgg actggcaaag atttccctct ggaatatgtt aaacaggtca
     4081 tggggttcgg tgcctgacag gctgaccgcg tgcagaaagg gtaaaaaatg aaaaccgtaa
     4141 ctgtaaaaga tctcgtcatt ggtacgggcg cacctaaaat catcgtctcg ctgatggcga
     4201 aagatatcgc ccgcgtgaaa tccgaagctc tcgcctatcg tgaagcggac tttgatattc
     4261 tggaatggcg tgtggaccac tttgccgacc tctccaatgt ggagtctgtc atggcggcgg
     4321 caaaaattct ccgtgaaacc atgccagaaa aaccgctgct gtttaccttc cgcagtgcca
     4381 aagaaggcgg cgagcaggcg atttccaccg aggcttatat tgcactcaat cgtgcagcca
     4441 tcgacagcgg cctggttgat atgatcgatc tggagttatt taccggcgat gatcaggtta
     4501 aagaaaccgt cgcctacgcc cacgcgcatg atgtgaaagt tgtcatgtcc aaccatgact
     4561 tccataaaac gccggaagcc gaagaaatca ttgcccgtct gcgcaaaatg caatccttcg
     4621 acgccgatat tcctaagatt gcgctgatgc cgcaaagtac cagcgatgtg ctgacgttgc
     4681 ttgccgcgac cctggagatg caggagcagt atgccgatcg tccaatcatc acgatgtcga
     4741 tggcaaaaac tggcgtaatt tctcgtctgg ctggtgaagt atttggctcg gcggcaactt
     4801 ttggtgcggt aaaaaaagcc tctgcgccag ggcaaatctc ggtaaatgat ttgcgcacag
     4861 tattaactat tttacatcag gcataagcaa taatatttcg gcgggaatat cctcccccgc
     4921 cgaactaaaa aaaatatatt caaccgtatt taataaaaat atttcgtgag tctctgtgcg
     4981 ctaattatcc atctggcgta gggaaaatca cacctgaatc aggaattaac aatgaaacct
     5041 gtaaaaccac ctcgtattaa tggacgagtg ccggtcctgt cggcacagga agcggtgaat
     5101 tatattcccg acgaagcaac actttgtgtt ttaggcgctg gcggcggtat tctggaagcc
     5161 accacgttaa ttactgctct tgctgataaa tataaacaga ctcaaacgcc acgtaattta
     5221 tcgattatta gtccaacagg gcttggcgat cgcgccgacc gtggtattag tccgctggcg
     5281 caagaaggtc tggtgaaatg ggcattatgt ggtcactggg gacaatcgcc gcgtatttct
     5341 gaactcgcag aacaaaataa aattattgct tataactacc cacaaggtgt acttacacaa
     5401 accttacgcg ccgccgcagc ccaccagcct ggtattatta gtgatattgg catcgggaca
     5461 tttgtcgatc cacgccagca aggcggcaaa ctgaatgaag tcactaaaga agacctgatt
     5521 aaactggtcg agtttgataa caaagaatat ctctattaca aagcgattgc tccagatatc
     5581 gccttcattc gcgctaccac ctgcgacagc gaaggctacg ccacttttga agatgaggtg
     5641 atgtatctcg acgcattggt tattgcccag gcggtgcaca ataacggcgg tattgtgatg
     5701 atgcaggtgc agaaaatggt taagaaagcc acgctgcatc ctaaatctgt ccgtattccg
     5761 ggttatctgg tggatattgt ggtggtcgat ccggatcaaa cccaactgta tggcggtgcg
     5821 ccggttaacc gctttatttc tggtgacttc acccttgatg acagtaccaa acttagcctg
     5881 cccctaaacc aacgtaaatt agttgcgcgg cgcgcattat tcgaaatgcg taaaggcgcg
     5941 gtagggaatg tcggcgtcgg tattgctgac ggcattggcc tggtcgcccg ggaagaaggt
     6001 tgtgctgatg actttattct gacggtagaa acaggtccga ttggcggaat tacttcacag
     6061 ggtatcgcct ttggcgcgaa cgtgaatacc cgtgccattc tggatatgac gtcccagttt
     6121 gatttttatc acggtggcgg tctggatgtt tgttatttga gttttgctga agtcgaccag
     6181 cacggtaacg tcggcgtgca taaattcaat ggcaaaatca tgggtaccgg tggatttatt
     6241 gatatcagtg ccacctcgaa gaaaattatt ttctgcggca cgttaactgc gggcagttta
     6301 aaaacagaaa ttgccgatgg caaattgaat atcgtccagg aaggacgggt aaataaattt
     6361 attcgggaac taccggaaat tacttttagc ggaaaaatcg ctctcgagcg agggctggat
     6421 gttcgttata tcactgagcg cgccgtattc acgctgaaag aagatggcct gcatttaatc
     6481 gaaatcgccc ctggcgtcga tttacaaaaa gatattctcg acaaaatgga tttcacccca
     6541 gtgatttcgc cagaactcaa actgatggac gaaagattat ttatcgatgc ggcgatgggt
     6601 tttgtcctgc ctgaagcggc tcattaacag gagtatcacg atggattttt ctttaactga
     6661 agaacaagaa ctgctgctgg ccagtattcg cgaactgatt acgactaact ttccggaaga
     6721 gtatttccgc acctgcgatc aaaacgggac atacccgcgt gaatttatgc gggcactggc
     6781 ggataacggt atttccatgc ttggcgtgcc ggaagaattt ggtggtatcc ctgcggatta
     6841 cgtcacccag atgctggcgc tgatggaagt gtcaaaatgc ggtgctccgg catttttgat
     6901 taccaacggt caatgtattc acagtatgcg ccgtttcggt tctgcagagc agctacgtaa
     6961 aacggcagaa agcaccctgg aaacgggtga tcccgcctat gccctggcgt tgacggagcc
     7021 aggcgcaggc tcagacaaca acagtgccac caccacttac acgcgtaaaa acggcaaggt
     7081 ttacatcaac ggacagaaaa cctttattac cggtgcgaaa gagtacccat atatgctggt
     7141 gctggcgcgc gatcctgaac cgaatgatcc gaaaaaggcc ttcacactgt ggtgggtcga
     7201 ctccagtaag cccggcatta agattaaccc gctgcataaa atcggctggc atatgctcag
     7261 cacctgcgaa gtctatctcg acaacgtgga ggttgaagag agcgacatgg tgggcgaaga
     7321 aggaatgggt ttcctcaatg tgatgtacaa ctttgagatg gagcgcctga tcaacgccgc
     7381 gcgcagcacc ggctttgccg aatgcgcctt tgaagatgcc gcccgctatg ccaaccaacg
     7441 tatcgctttt ggtaaaccca ttggtcataa ccagatgatc caggaaaaac tggcgctgat
     7501 gcgattaaga tcgacaacat gcgcaacatg gtgctgaaag tagcatggca agccgatcag
     7561 catcagtcac tgcgcaccag cgcggcgctg gcaaaactgt attgcgcacg taccgcaatg
     7621 gaagtcattg atgatgcgat tcaaatcatg ggcggtctgg gctataccga tgaagtgcgc
     7681 gtctcccgct tctggcgtga tgtccgttgt gaacgtatcg gcggcggtac agacgaaatt
     7741 atgatttacg tagcaggtcg gcagatcctg aaagactatc agaacaaata atctgcaggc
     7801 ggcgcagctt cttaacaaac tgcgccgcca gatttatcca acaagactta ccggttgagg
     7861 aaattcagat aggatttctt gttccgcaaa acagttttta aattgcctgc ggtaatcgct
     7921 gggtgaacag ccgacatggc gcaaaaacag tttgccaaag tgatcgacat tttcataacc
     7981 cacacgccag gagatctctg cctgtgataa ttcagtatta gtgagtaacc atttcgcttc
     8041 cgtcatacgc cgttgaataa catagttaat tggggaaata cgatactctt tggtaaattc
     8101 atggcaaata taactgacgc tggcacgaaa ttttttcgat aactgttcta aagtg
//
LOCUS       DAZUGK010000196         8150 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZUGK010000196 DAZUGK010000000
VERSION     DAZUGK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.197"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..1772)
                     /gene="treA"
                     /locus_tag="ACHY5W_003880"
                     /pseudo
     CDS             complement(76..1772)
                     /gene="treA"
                     /locus_tag="ACHY5W_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            complement(2093..3511)
                     /gene="dhaM"
                     /locus_tag="ACHY5W_003881"
     CDS             complement(2093..3511)
                     /gene="dhaM"
                     /locus_tag="ACHY5W_003881"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4448680.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRALVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(3522..4154)
                     /gene="dhaL"
                     /locus_tag="ACHY5W_003882"
     CDS             complement(3522..4154)
                     /gene="dhaL"
                     /locus_tag="ACHY5W_003882"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4448681.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4165..5235)
                     /gene="dhaK"
                     /locus_tag="ACHY5W_003883"
     CDS             complement(4165..5235)
                     /gene="dhaK"
                     /locus_tag="ACHY5W_003883"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4448682.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGNWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5463..7382
                     /gene="dhaR"
                     /locus_tag="ACHY5W_003884"
     CDS             5463..7382
                     /gene="dhaR"
                     /locus_tag="ACHY5W_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFS4448683.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7482..>8150)
                     /locus_tag="ACHY5W_003885"
     CDS             complement(7482..>8150)
                     /locus_tag="ACHY5W_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4448684.1"
                     /translation="PLYVGLYIGSTHASPDYSGGDGTARSDYMGIYASYMAHSGFYSD
                     LVVKASRQKNSFHVRDSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQL
                     TYSHQNEMAMKASNGLNIHLNHYESLLGRASMILGYDITAGKSQLNIYVKTGAIHEFS
                     GDTEYLLNNSREKYSFKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGY
                     RFSF"
BASE COUNT         1920 a         2040 c         2080 g         2110 t
ORIGIN      
        1 ggggggcatt tcgtggcgaa aaaagagcaa tgccaatttt atgcacataa tggcattgct
       61 cgatatacag caaagttaag gcgtaggttc cgcctctttt tgttttaccg gctgtgttgt
      121 tgattcactg agtggacgcg tcgccggaac attgtcacac ggttgctctt tcgggcagat
      181 caaatccagc attttcagcg tcacgccatt ggtccagcca aagccatcct gtaatggata
      241 ttcgccaccg ccgcccccgt tccggtggtg ctgacatcat atttttccac cagctttttc
      301 tcccggtcat aggtgtgctg aacattggtc aggaagtgcc agctaatgtc catcgccacc
      361 tctttttgcc cgtagttttg taatccttct gtcgcgaccc actgcaacgg tgcccagcca
      421 tttggcgcat cccattgttg cccacttttc accgacgtgg tgttcaggcc gcctggttgc
      481 agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc tgccgcattg
      541 acgtacagcg ggaacagggc ggcagcggtt aactgattgc gcactttatg acttttcagg
      601 tcgtaatcgg cataccagcc ttgttgatcg ttccacaggt atttttcgat ccctttttga
      661 cgggcatttg ccagtgtttc gtactggttt gccatcgcgt tatctccggc agctttgctg
      721 gcgcgggcaa ggattttttc cattttgaac atcaggctgt tcagatcgac cggtacgatg
      781 ctggtggtgc gtaaggtatt tagttgctgc gggttgtcca tccagcgcga gctgaaatcc
      841 cagccagacg cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga
      901 ttgcttttag cggtggcaat atcttccacc catgactctg gtcgtggcgt atcgcgatcg
      961 tcccagtagc ggttgagaag ggtaccatcc tgaagtttta caacgcgttt ttcctgttgt
     1021 ccggcttgca ggttttcaac accgtccatc cagtaagcat attctttttg catttgcggc
     1081 aggtattgct tcaacgcggc atcgccttca tgctgcgcca gtaactctac catcagggca
     1141 aagaagggcg gctgcgagcg gcttaaatag taactgcggt tgccgttggg aatatgaccg
     1201 taagtgtcta tttcatgagc aaaattggcc accatatccg cgactttatc ccagtgaccg
     1261 ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt
     1321 ccgcccggca cgacataagg ttccggcagc ggtaacagag aatcccattt ttcggtgttt
     1381 tcggtagaac gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag tgactgtccc
     1441 tctggcggaa catatttctc gccctctgtc ggcagggtga aattgacgtt aacgaaatgg
     1501 cgcagatcaa atccgctctg gttttgctgc atccgataat cagcaaggat catcagcgga
     1561 tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc
     1621 acatcattaa acagcggccc taataaaata tcaggcggct gtggcgttac cggtgtttct
     1681 tctgcctgca ccgatagcgc agcgaaacac aaaaagatgc aggctggaat taacgccatt
     1741 ttttgcgggc gagaaggtgc gggggatttc atcaatcatt ctccttaggc gaaaccgaat
     1801 aaaacggtat tcaagtatca gaaaacctta gttcaggatc gcactagctg catgatcgaa
     1861 atctcattct gcgaactgtc aggcatttta gaaaagggaa ttaaagggat aaataacggc
     1921 gaaatagagt gatcaaataa cgtctgttta ttagccagcc agcggcgttt ggttcgcgat
     1981 tagcgaagaa aaagtcatca taaattggtg aaaaatataa caggtagtta aaaaacatta
     2041 gtgctgagta aattgccgga tgacatcaga acgatgccat ccgaacggtg gcttaaccct
     2101 gacggttgaa acgttgcgtt ttaacgtcca gcgttagctt ttcttctggt tgtatcgcat
     2161 acagtttctc accctgctgg caaatccagc caatccccag ttcacgggcg atcagggcgc
     2221 tgtgggatac cggacttccg gcgctaaggc agataccttt tacaaccgcc ggatccagtt
     2281 gcagtactgt ggaaggataa atgttctccg ccagtagaat agttggcgag ttaaactgcg
     2341 ggagttcttc tttcgtttgg gtcaggtgga ccagggcgcg atgcagaaga tcgtccacat
     2401 caatatagcg agcttgtaga tattcatcat ccagttgctg gtattgctgg ctaagttctt
     2461 taagaacttg ctgccaggca tattctgccg tgcaatgttc gtgctgaagg agttcgcttg
     2521 ccgccgccag cagttccgga tcatctaaca gtgtatggtg accagaaaag attgcggcaa
     2581 tatcgtcaag cccgctggct tctgctttcg ctgttaacgt catcagatct aataacgtga
     2641 agtcaatggc ctggcgtaat cgatcttgtt cctcttccac ggtcagggtt gatttggcct
     2701 gtaccgtaca taaaaccggt tgataataaa aggctttacc cgaaacaggc ggaacgggac
     2761 gcagagtagg tggagcaact tcctcagttt caccaaagtt atcttcagcc agctgacgga
     2821 aagcgatcag tgcctcttca gcttctggcc ctttcgcaat caggcgcagc gtatcgttat
     2881 agcgaacttg tagtaacgca atctggttaa tactctccgg tgtgacgcat ttgccgtttt
     2941 tctccagcaa catatcggca ttaaatgtcg ataaggtata aaccagccgg gaggctggac
     3001 gtacatgcag gccgttacgg ttctttatga ccaccgccag agaacgggct tcttcatcgt
     3061 acggaggaca tgtgtcagag atttcagtgt cggaggacgg taaacccagt tgttcacgtt
     3121 tggcttccag cgcatgcatg gcgtcaaaga taactttgtc gatatccgcc cccgaggccg
     3181 cgctgaccgt tgctgccagt gtaccttcga ccaacggcgc agcacacaaa cgtacttttg
     3241 cggcgatctc gggagccagc aattccagcg cagtttcagc actcaataat gcgctaccca
     3301 tatccatcat gaccagcaca tggtcggcat cggcaacaga ttctatggcc tccatcactt
     3361 tgacggcatc ggtaccaatg ggattttgtg gatcgtcaat tcccgcagca atggcgattt
     3421 tacaattatc actcattaac atctgacggg ctaattcacc gacaccttct cccagtcggc
     3481 tgctatgtga aactatgacc aggtttacca tcaccaattc cttactcttt tgcggctaac
     3541 gccaacattt gcatcataaa catcaccgag gtcgcgccgg gatcctggtg accaatactg
     3601 cgttcaccga gataactggc gcggcctttg cgggcttgca tcgtaatcgt actttgtgca
     3661 gcggattcgg cgatgctact ggcagcttcg agcgccgccg gaacagagag attttgctcg
     3721 ctggactgac gtaacgattc caccaccggc acccacacat cacacatggt tttatcgcca
     3781 ggttcggctt tcccgcgact gattacgccg tccgcaccat cgcggaacat ctgataaagc
     3841 tcttccagcg tcaggctttg ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta
     3901 ccgaacagcg gaccactggc accaccgacg ctggaaagca gcgtcatacc ggtattcttg
     3961 agaatgaaac cgatatcttt atctgcgata gcagggagtt tttccaccac tttgctaaag
     4021 cctcggttca tatttagccc gtggtcagcg tcgccaatct cgcgatccag tccggtaaga
     4081 tactcgctct cggtgttgaa aatatcgcca caacgagtga gccagttaac aatttgagtt
     4141 ctgctcagtg acattgcttt ctccttattt accccagtta agggccgggg tgtggacagg
     4201 ggcgtcccag agtgccagcg tttcgtcatc aactttcagt aaggtgattg agaaaccggt
     4261 catatccagt gaggtgcagt acgcgccaat taaattacgc tcgatagtca atcccgcttg
     4321 ctggcaacgt gtggtcaggc ggttatagac gccgtacagc tcagaaagtg gagttgcgcc
     4381 aagattgtta accagcgcaa tcaccctatc gccagactgg agcggttgtt tggtttgtgg
     4441 ttcttcctgc cagttgcctt gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc
     4501 attttccagc agggtgtcga acatttcatc gacggtttga tcaagggaag agaaggggcg
     4561 gcggtcaata cccggctcac catgaatgcc gacgccaaac tccatctcat tatccgccag
     4621 ggtaaaagaa ggtttgcccg cggcaggaac ggtacaggca ccgagagcga tacctattga
     4681 gtggccttga ttattcaact tacgccccag ttccgcacag gcgtccagtg agtcgccacg
     4741 ctccgccgca gcgcctacga gtttttcaat taatacggtg ttggcaacgc cgcgccgccc
     4801 ggcggtataa agactgtctt ttactgcaac gtcgtcatca atgaccacag tggtcacttt
     4861 tacgccgcta tcgtgcagta actcggtcgc tgtttcaaag ttaagaatat cgccggtgta
     4921 atttttgata atcaacagta caccttcgcc gccatcaact tgcatggcgc attcaaagat
     4981 tttatcgggc gtcggtgagg tgaaaatttc gcccggacag gcccccgaaa gcatcccctg
     5041 accgatataa ccacagtgca tcggctcgtg tccgctaccg ccacccgaca gcagggcgac
     5101 ttttcctgta acgggggcat cagctcgggt gacatacacc ggatcctgat gcagtgtcag
     5161 cgatggatgc gctttcgcca gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt
     5221 gatcaatttt ttcattattt tgctccagca attacggtag ggcatggatg atgttcaacg
     5281 acacggcgac cactgactgc cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt
     5341 agcccaaatt atgtttcata gtgaaacata tgctttaatg aatgttccat attgaaactt
     5401 ttacgtgtat taatacttaa aattgcgagc cggaacacct tttgtcataa gggatgcggg
     5461 atatgagtgg cgcttttaac aacgatggtc ggggcatatc tcccttaatt gcaacctcct
     5521 gggagcgatg caataagctg atgaaacggg agacatggaa cgtaccacat caggcccagg
     5581 gcgtgacatt tgcttctatt tatcggcgta agaaagcgat gctgacgctc gggcaggctg
     5641 ctctggaaga tgcctgggaa tatatggcac cgcgagagtg tgcgctgttt atcctcgatg
     5701 aaaccgcctg cattctcagc cgtaatggcg atccgcaaac cttgcagcag ctaagtgcac
     5761 tgggattcaa tgacggcacg tattgcgccg agggaattat tggtacttgt gcgctatcgt
     5821 tagcggctat ctctggtcag gccgtgaaaa cgatggccga tcaacatttc aaacaggcac
     5881 tctggaactg ggccttttgt gcaacgccgt tgtttgacag caagggccga ttgacgggaa
     5941 caatagcgct ggcgtgtccg gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg
     6001 caatcgcccg cgaggtcggt aatttactgc tgacggacag tttgctcgct gaaactaacc
     6061 gtcatttaaa tcaacttaat gccctgttag aaagtatgga tgatggcgtg attagctggg
     6121 acgagcaggg taatttgcaa tttattaatg cccaggcggc gcgggtcttg cgccttgacg
     6181 cgacggcaag tcagggacgg gcaatcactg aactcttaac gttacccgcc gtattgcaac
     6241 aagcaataaa acaggcacat ccgctcaaac acgtagaagc aacctttgaa agccagcacc
     6301 agtttattga tgcggtgata acccttaaac cgataataga aacgcaggga accagcttta
     6361 ttttgttgct ccatcctgtg gaacagatgc ggcagttaat gaccagtcaa ttaggaaaag
     6421 tcagccatac cttcgctcat atgccacagg acgatccgca aacccgccgc ttgattcatt
     6481 ttggtcgcca ggcagcgcgc agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg
     6541 gcaaggcact gttaagtcag gcaattcata atgaaagcga gcgtgctgca ggtccttata
     6601 tcgccgtcaa ttgtgagtta tatggtgatg cggtgctggc ggaagaattt attggcggcg
     6661 atcgcacgga caatgaaaat ggccgtctga gtcggctgga actggcgcac ggcggcacgc
     6721 tgtttcttga aaagattgaa tatctggcgg tggagttaca gtctgctttg cttcaggtta
     6781 tcaagcaggg tgttatcacg cgactggatg cgcggcgttt aataccaatt gatgtcaaag
     6841 tgattgccac aacgaccgcg gacctcgcaa tgctggtgga acaaaatcgt tttagtcgcc
     6901 agctgtatta cgcgctgcat gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg
     6961 gcagcattcc ggctctggtg aataacaaat tacgcagtct tgaaaaacgc ttctctacgc
     7021 ggctgaaaat tgatgacgat gccctcgctc gcctggtttc ctgtgcatgg cctggcaacg
     7081 attttgaact ttacagcgtc atcgagaatc ttgctctgag tagtgataat gggcgcattc
     7141 gcgtcagtga tttgccggaa catctgttta ccgagcaggc aacagatgat gtcagcgcca
     7201 cacgcctttc caccagtctg tcatttgcgg aagttgaaaa agaggcaatt attaacgcag
     7261 cccaggtcac aggcggtcgc attcaggaaa tgtcggcttt acttggaatc ggccgcacta
     7321 cgctgtggcg gaaaatgaag caacatggca ttgatgcagg gcagtttaag cgccgggtat
     7381 gaaagacaga gacgatttct gatacatcag agtgatctat atttcattcc ggcgcacgct
     7441 aacatttttc agtatcgttt aagggcttgt ctatcccgca cttaaaagct gaagcgatat
     7501 cctccgttga cttgcttctg atcaaagagg ttaccctgcg tgtaatccgc ttcgaggtag
     7561 aatgtgtgct gtttgttata ctgtgcactg actcccacgc cattattcca gccattacct
     7621 ttgaaactgt acttctcccg ggagttgttc aacagatatt ctgtatcccc tgaaaactcg
     7681 tggatagcgc cagtcttcac atagatattc agctggcttt tacctgcggt gatgtcatat
     7741 cccagtatca tgctggcacg ccccagcagc gattcgtagt gattcagatg tatattgaga
     7801 ccattactcg ccttcatagc catctcattc tggtggctgt atgtgagctg ggtttgcggc
     7861 tctatataga acccataacc agtaggggtc aggttgaacc tctgcccggc ttccagggag
     7921 atgctcaatc cattcgcagt gccgttggcg ttaacgccgt tgttctgact gtcccgtacg
     7981 tggaaactat ttttctggcg cgatgctttt acaacgagat cgctgtaaaa accgctgtgt
     8041 gccatgtaac tggcgtatat tcccatatag tctgaacgtg cggtaccgtc gcctccgcta
     8101 tagtccggcg atgcatgtgt tgagccaata tacagaccga catacaacgg
//
LOCUS       DAZUGK010000197         8079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZUGK010000197 DAZUGK010000000
VERSION     DAZUGK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.198"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..629
                     /gene="ecpR"
                     /locus_tag="ACHY5W_003886"
                     /gene_synonym="matA"
     CDS             87..629
                     /gene="ecpR"
                     /locus_tag="ACHY5W_003886"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFS4448685.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            704..1291
                     /gene="ecpA"
                     /locus_tag="ACHY5W_003887"
                     /gene_synonym="matB"
     CDS             704..1291
                     /gene="ecpA"
                     /locus_tag="ACHY5W_003887"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFS4448686.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1349..2017
                     /gene="ecpB"
                     /locus_tag="ACHY5W_003888"
                     /gene_synonym="yagY"
     CDS             1349..2017
                     /gene="ecpB"
                     /locus_tag="ACHY5W_003888"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFS4448687.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2043..4567
                     /gene="ecpC"
                     /locus_tag="ACHY5W_003889"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             2043..4567
                     /gene="ecpC"
                     /locus_tag="ACHY5W_003889"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            4557..6200
                     /gene="ecpD"
                     /locus_tag="ACHY5W_003890"
     CDS             4557..6200
                     /gene="ecpD"
                     /locus_tag="ACHY5W_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFS4448688.1"
                     /translation="MRVNLLITMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6169..6879
                     /gene="ecpE"
                     /locus_tag="ACHY5W_003891"
     CDS             6169..6879
                     /gene="ecpE"
                     /locus_tag="ACHY5W_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFS4448689.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7192..7521
                     /gene="ykgJ"
                     /locus_tag="ACHY5W_003892"
     CDS             7192..7521
                     /gene="ykgJ"
                     /locus_tag="ACHY5W_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS4448690.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYQNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7769..>8079)
                     /locus_tag="ACHY5W_003893"
     CDS             complement(7769..>8079)
                     /locus_tag="ACHY5W_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HFS4448691.1"
                     /translation="GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMI
                     SFPLMGLTPPLFDLPWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR
                     "
BASE COUNT         2133 a         2058 c         2062 g         1826 t
ORIGIN      
        1 actgaatgta cctgtaaaaa ttacaggttt ggaaagtagt gacatggcaa aatgattaca
       61 gcagggacta tgaggttaaa aaccatatgg aatgtcaaaa ccgttctgat aaatacatct
      121 ggtctcccca tgacgcctac ttctataaag gactatctga actgattgtg gatatcgaca
      181 gattaattta tctatcgctg gagaaaatca gaaaagattt cgtgtttatc aatctcaata
      241 cggattcttt aactgaattt ataaaccgtg ataatgagtg gttatccgcg gtaaagggga
      301 aacaggtcgt attgattgcg gccagaaagt cagaagcctt agcaaattat tggtattaca
      361 acagcaatat taggggcgtg gtatacgctg gactgagtcg tgatattaga aaagaactgg
      421 cctatgtgat taatggcagg ttcctgagaa aagatattaa gaaagataaa atcactgacc
      481 gggaaatgga aattatccgc atgacggctc agggaatgct gcctaaatcg attgccagaa
      541 ttgaaaattg tagtgtaaag acagtgtata cccatcggcg taatgcagag gccaagctgt
      601 actcaaaatt atataagttg gttcagtaaa ctccaggcaa gttagtttta ataaatgact
      661 caccgggact tcatgtcctc aattcaactc gggaagaaaa gcaatgaaaa aaaaggttct
      721 ggcaatagct ctggtaacgg tgtttaccgg tataggtgtg gcgcaggctg ctgacgtaac
      781 agctcaggct gtagcgacct ggtcagcaac agccaaaaaa gacaccacca gtaagctggt
      841 tgtgacgcca ctcggtagcc tggcgttcca gtatgccgaa ggcattaaag gttttaactc
      901 acagaaaggt ctatttgacg tggctatcga gggtgactca acggctaccg cctttaaact
      961 gacctcacgt cttatcacca acacattaac ccagttggat acctcaggtt ccacactgaa
     1021 tgtgggcgtg gattataacg gcgcggcagt cgaaaaaact ggcgataccg tgatgatcga
     1081 taccgccaac ggcgtactgg gcggcaacct tagcccgctg gctaacggtt acaatgccag
     1141 caatcgtacc accgcacagg atggtttcac cttctccatc atcagcggca ccaccaatgg
     1201 taccaccgca gtaaccgatt acagcactct accggaaggc atctggagcg gcgacgttag
     1261 cgtacagttc gacgcgacct ggaccagtta atctctctga tgtaccagca ggggtagccc
     1321 ccctgcttta ttcctggacg gaattgttat gaaaaagcac cttctgcctc tcgctctgct
     1381 gttttccgga atatctccgg cccaggcgct ggatgtcggc gatatatcat cgtttatgaa
     1441 cagtgacagc agcacgctga gcaaaacgat caaaaacagt accgacagtg gtcgcctcat
     1501 caatatccgt ctcgaacggc tctcttcacc gcttgacgac gggcaggtta tctcaatgga
     1561 caagccggac gagttgctac tcactcccgc cagcttgctg ctacccgccc aagccagcga
     1621 agtgatccgc ttcttctata agggacccgc agatgaaaaa gagcgctact accgcattgt
     1681 ctggtttgat caggccctca gtgatgcgca gcgcgataat gccaaccgca gcgctgtggc
     1741 cactgcttcc gcccgcatcg gcaccattct ggtcgtcgca ccccgccagg caaactacca
     1801 ctttcagtac gccaacggct ccctgacaaa tacaggaaat gcgacgctgc ggatcctcgc
     1861 ctacggcccc tgcctgaaag ccgccaatgg taaagagtgt aaagagaatt actacctgat
     1921 gccgggcaag tcgcgtcgtt ttacccgcgt ggacacggcg gataacaaag gacgggttgc
     1981 actttggcag ggtgataagt tcattcccgt gaaatagata gctgtgcaga tggataacga
     2041 caatgccttt acgacggttc tccccaggac tgaaagccca gtttgccttc ggcatggtct
     2101 ttttgttcgt tcagcccgat gccagcgctg ctgacataag tgcgcagcaa ataggtgggg
     2161 tgattattcc gcaggccttc agtcaggcgc ttcaggacgg catgagcgtc ccgctctata
     2221 ttcatctcgc cggtagtcag ggtcgccagg acgatcagcg aatcggcagc gcttttatct
     2281 ggctggatga tggacagcta cgcatccgga aaatacagct ggaagagagt gaagataacg
     2341 ccagtgtcag cgaacaaact cgacagcagc tgatggctct ggcaaacgcc ccgttcaatg
     2401 aggcccttac catccccctg actgacaacg cgcagctgga tctcagcttg cgccaactgc
     2461 tgctgcagct ggtggtcaag cgcgaagcgc tgggcactgt actacgctca cgtagcgaag
     2521 acatcgggca gtccagtgtt aacaccctca gcagtaatct gagctataac ttcggcatct
     2581 ataacaacca gttgcgtaac ggcgggagta acacatccag ctatctgtcg ctgaataacg
     2641 ttactgcact gcgcgaacat catgtggtgc tcgacggctc gctgtacggg atcggtagcg
     2701 gtcaacagga cagtgaatta tataaagcga tgtatgaacg cgattttgcc ggtcaccgat
     2761 ttgccggtgg aatgctcgac acctggaact tgcagtcctt agggccgatg accgccattt
     2821 cagcagggaa gatttacggc ctttcctggg gaaaccaggc cagctccacc atcttcgaca
     2881 gcagccagtc agccacgcca gtgatcgcct ttttaccggc ggcgggcgaa gtacatctca
     2941 cccgtgatgg gcggttacta agcgttcaga acttcaccat gggcaatcat gaagtggata
     3001 cccggggtct accgtacggt atttacgatg tggaagttga ggtgatcgtt aacggtcgcg
     3061 tgatcagcaa acgcactcaa cgggtcaata agctgtttag ccgggggcgc ggcgtcggtg
     3121 caccactggc gtggcagata tggggcggta gctttcatat ggatcgctgg tcggaaaacg
     3181 ggaaaaagac gcgaccagct aaagagagtt ggctggcagg tgcctcgacc tccggctcac
     3241 tgagtacgct tagctgggcg gcaacgggat atggatacga taatcaggcg gtgggtgaaa
     3301 cccgtctgac gctgccgctt gggggagcga tcaacgttaa cctgcaaaat atgctggcca
     3361 gtgacagctc atggagcaac atcgccagca tcagcgccac tctaccggga ggctttagtt
     3421 cgctgtgggt taaccaggaa aaaacccgca ttggcaatca attgcgacgt agcgatgccg
     3481 acaaccgtgc aatcggcggc acactcaacc tgaactcact gtggtcgaag ctgggcacat
     3541 tcagcatcag ctacaatgat gaccgccgtt acaacagcca ttattacacg gcagattact
     3601 atcaaaatgt ctacagcggt ccctttggtt cgcttggcct gcgggccggt attcagcgct
     3661 ataacaacgg cgacagcaac gccaatacag ggaaatatat cgctctcgat ctctcgctac
     3721 cactgggcaa ctggtttagc gcagggatga ctcatcaaaa cggctacacc atggcaaacc
     3781 tgtcagcacg caagcagttt gatgaaggga ccattcgcac tgttggtgcc aatctgtcac
     3841 gagccatctc cggcgatacc ggtgatgaca aaactctcag tggtgggcgt atgcacagtt
     3901 cgacgctcgc tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg gctacgtcaa
     3961 taccaattta accgccaatg gcagcgtcgg ctggcagggt aaaaacattg ctgccagcgg
     4021 gcggactgat ggcaacgctg gggtgatatt caacaccggg ctggaggacg acggtcagat
     4081 cagcgccaaa atcaacgggc ggattttccc gcttaacggc aagcgtaact atctcccgct
     4141 ctctccctat ggaagatatg aggtggagtt acagaacagc aaaaactcac tcgacagtta
     4201 cgatatcgtc agcggccgca aaagccatct gactctctat ccaggcaatg tcgctgtcat
     4261 tgagccagag gtgaagcaga tggttaccgt ctccggtcgt atccgtgcgg aagacggcac
     4321 actgctggct aacgcacgga ttaacaacca tatcggccga acccgaaccg atgaaaacgg
     4381 cgagtttgtc atggacgtgg ataagaaata ccccactatc gattttcgct acagtggcaa
     4441 taaaacctgc gaagtggcac tggaactcaa ccaggcgcgc ggtgccgtct gggtcggtga
     4501 tgtggtctgc agcggcctct catcgtgggc ggcggtgacg cagacaggag aagagaatga
     4561 gagttaacct actaataacg atgataatct ttgcgctaat ctggcctgca accgcgctca
     4621 gagctgcagt gagcaaaaca acctgggcgg atgcaccggc acgcgagttt gtgtttgtcg
     4681 aaaacaactc agacgacaac tttttcgtca ctcctggcgg ggcgctggat ccgcgcctga
     4741 ccggtgccaa ccgctggacc ggtttaaaat acaatggttc aggaaccatc tatcagcaaa
     4801 gcctcggcta cattgataac ggttacaaca ccggccttta taccaactgg aagtttgata
     4861 tgtggctgga aaattcacca gtttcatctc ctttaactgg cttgcgctgc atcaactggt
     4921 acgctgggtg taatatgacc accagtctta tcctgccgca aaccaccgac gccagtggat
     4981 tttatggcgc gacggtgacc agcggcggcg cgaagtggat gcacggcatg ttgtcagacg
     5041 cgttttacca gtatctgcaa caaatgcccg tcggcagcag ctttacaatg accatcaatg
     5101 cctgccagac ctctgtgaac tatgacgcca gcagcggcgc acgctgtaag gatcaggcct
     5161 ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc agcaaatcta cggttgataa
     5221 atacccactc gctggcggaa gtatttatca acagcgacgg agtaccgact ctgggcgaag
     5281 ggaacgccga ctgccggacg caaaccatcg gcagccgttc aggattaagt tgtaagatgg
     5341 ttaactatac cctgcaaaca aacggactca gcaacacctc aatccatata ttcccggcga
     5401 tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc agtctgaatg
     5461 gcagttcatg gaaaccggtg agcaatactg cctattacta caccttcaac gagatgaaga
     5521 gcgcagactc gatctatgtt ttcttctcga gcaacttctt taagcagatg gtgaacctcg
     5581 ggatcagcga tatcaacacc aaagatctat tcaactttcg ctttcagaac accacatcac
     5641 cggagtctgg ctggtatgaa ttttctacct ccaacacgct gattatcaaa ccccgtgatt
     5701 tcagcatcag tattatctcc gatgaatata ctcagacacc gtcgcgggag ggatatgttg
     5761 gcagcggcga gtcggcactc gatttcggct atatcgtaac caccagcggt aaaacagctg
     5821 ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt gattggcggg cgctcctatt
     5881 gtgtcttcag ctccgatgac ggtaaggcga aagtaccatt cccggcgacg ctttccttta
     5941 ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc tggcgggata
     6001 tgaccgatgc gctgtggttg accacaccgt ggactgatat ctctggcgaa gtggggcaga
     6061 tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttcg ctgcgtacgg
     6121 tagatgataa cggctggttt ggcgaagtca gcgcttcagg agaaattcat gttcaggcga
     6181 cgtggcgtaa cattaactaa ggccctgctg acagcggtct gtatgctggc ggcacctttg
     6241 acacaggcga tttcggtcgg caatctgaca ttttcgctgc cgtccgagac tgactttgtc
     6301 agcaaacgtg tagtgaataa caacaaaagc gcacggatat accgtattgc catcagtgct
     6361 attgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga actgcttttc
     6421 gccccccgcc agctggcgtt gcaggctggt gagagcgagt attttaaatt ttactatcat
     6481 ggtccacagg ataaccgcga gcgctactac cgggtctcat ttcgcgaggt ccccactcgt
     6541 aaccagacaa gacgcagccc aaccggcggc gaggtcagca cggagccggt ggtggtgatg
     6601 gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt cgacaaggtg
     6661 acaggaacgg taagtaacac tggcaacaca tggtttaagc tactgattaa gccaggatgt
     6721 gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt ggttcatcag
     6781 cctgagttac gtcagccggg gaatcattat ctggtctata acgacaaatt cattaagatt
     6841 agcgattcct gtccggctaa gcccccttcg gcggactgat acttatccat cggtcgaggg
     6901 aagaattcca cctcagagac tcaaaaaacg tttaaatgat gtggaagcga tcgtcaatgt
     6961 cacctgtaac gatgggcttt ctcatatgca caccatagtg ttcaggagac acagaaaggt
     7021 atcaggctgc ctgctaacga gtggtttatt cacgaaatag tacgtaaaat cggtaacggc
     7081 tggaaatcat tcaatactca cactatcgaa agtacaccag ccaaccgcgg tacgttctta
     7141 catacgatgt accgctgttc tctttacgat ttatagctgt actggtgaat tatgagcaat
     7201 ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc tttttactgg
     7261 gccgaagccg acgatgctgg cggtaccatt cccgccaggc tcactgaaca aatatcccct
     7321 tttcaccgat gcatgagcgg taccaatcag aaaaaccccc gatgtattgc ccttgcagga
     7381 actccgggca aaaatgcctg ctgcacgata tatcaaaatc gatcgtccac atgcagagaa
     7441 ttcgccatgt ctggtgagaa cggagtcgtc aatgaggctt gcaatcgtgc aagggctaaa
     7501 tacgggctga caccgttata aacatacaat aattaattgc actgccccgc cagcgataat
     7561 agcggggctt cgtttttcag gggtaacaaa acccgaaact tcttctattt gccagcaaca
     7621 atgcctctct tctgtagcgt tcttcggatc tacatcatcc tgaagtagcg cgatatactc
     7681 actgactatc tgaatcagaa tataaaaagc aatgttttta acctataaaa atggcgctgt
     7741 atttgcgcca tttttatcat tcaatgcatt atctgtttga gcctaaaggg atctcagggt
     7801 ctggctcatg agtaattctg tttcgtaaat ctctgcgaat aatttcaatg gaccagaacc
     7861 agactaaatg tccaaaaatt tcagaaacat tctcatacca cgggagatca aacagaggtg
     7921 gtgtcagtcc catgagaggg aatgaaatca tatgaacaaa aagttgggct aaagcacctg
     7981 ccagtaagcc ctgccagagt ttaatttttg gaaatacttc agcgaccaca caataaccga
     8041 cagcaaacac tatcgaaaag ataatgtgcg taacaccaa
//
LOCUS       DAZUGK010000198         7993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZUGK010000198 DAZUGK010000000
VERSION     DAZUGK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.199"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..173
                     /locus_tag="ACHY5W_003894"
     CDS             <1..173
                     /locus_tag="ACHY5W_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4448692.1"
                     /translation="PDTDGALITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGL
                     DEVGSDTGRTGE"
     gene            175..444
                     /locus_tag="ACHY5W_003895"
     CDS             175..444
                     /locus_tag="ACHY5W_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4448693.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            847..1860
                     /gene="nleC"
                     /locus_tag="ACHY5W_003896"
     CDS             847..1860
                     /gene="nleC"
                     /locus_tag="ACHY5W_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFS4448694.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYADPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1981..2064)
                     /locus_tag="ACHY5W_003897"
                     /pseudo
     CDS             complement(1981..2064)
                     /locus_tag="ACHY5W_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            2149..2235
                     /locus_tag="ACHY5W_003898"
                     /pseudo
     CDS             2149..2235
                     /locus_tag="ACHY5W_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(2232..2480)
                     /locus_tag="ACHY5W_003899"
     CDS             complement(2232..2480)
                     /locus_tag="ACHY5W_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4448695.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2706..2801)
                     /locus_tag="ACHY5W_003900"
                     /pseudo
     CDS             complement(2706..2801)
                     /locus_tag="ACHY5W_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2798..3364)
                     /gene="yecD"
                     /locus_tag="ACHY5W_003901"
     CDS             complement(2798..3364)
                     /gene="yecD"
                     /locus_tag="ACHY5W_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4448696.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPEYWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3674..5446
                     /gene="aspS"
                     /locus_tag="ACHY5W_003902"
     CDS             3674..5446
                     /gene="aspS"
                     /locus_tag="ACHY5W_003902"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4448697.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDSEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            5564..6016
                     /gene="nudB"
                     /locus_tag="ACHY5W_003903"
     CDS             5564..6016
                     /gene="nudB"
                     /locus_tag="ACHY5W_003903"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFS4448698.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETASQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            6045..6785
                     /gene="yebC"
                     /locus_tag="ACHY5W_003904"
     CDS             6045..6785
                     /gene="yebC"
                     /locus_tag="ACHY5W_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4448699.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6820..7341
                     /gene="ruvC"
                     /locus_tag="ACHY5W_003905"
     CDS             6820..7341
                     /gene="ruvC"
                     /locus_tag="ACHY5W_003905"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFS4448700.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(7343..7945)
                     /gene="yebB"
                     /locus_tag="ACHY5W_003906"
     CDS             complement(7343..7945)
                     /gene="yebB"
                     /locus_tag="ACHY5W_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFS4448701.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         2083 a         1848 c         2103 g         1959 t
ORIGIN      
        1 tcccggatac agatggtgcg ctcatcaccg gactgacctt tcttgacccc aaagatgcca
       61 cacaggttca ggggctgttt cggcatttgc aggtcaggtt tggtgacggg ccgtggcagg
      121 atgttaaggg gctggatgaa gtgggcagtg atacaggcag aacaggagaa tgacatgaac
      181 atactaaaaa aacttatgca gcgtctgtgt ggttgcggaa agcatgatga ccgtgaacac
      241 ggggagttac ttacagcaca gctgcgactg ggaccggcag acattctgga gtcagatgag
      301 aatggcatta tcccggagca ggacagggta atcacgcagg tggtgatact ggatgcggat
      361 aaaaagcaga tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg
      421 gaaaatattg gcgggatgaa gtaacccgac agcttcacaa aaccggagtc cggctccggt
      481 ttttgtgttg caatgtccgg gggatatttg ttaagtagat gatagaggag ctaattcaac
      541 agggagataa attaatgccg ataaatctga catcttattt ggggttacag ggggcgaaag
      601 ttgtcccggc agttgttttt tctaaaattt agtcttgttg gggtaaatga catatgccat
      661 tgataacatc cagcatttca tcaaatgttg ctttgcagag tattgaaata ttacgtgaag
      721 ctgccagaca aaacctgata acgaaagata ttactataaa tgggcagaaa gttggtattc
      781 attattatca acgccctgac gtttttttag tttctggttg taagctagtg aattggtggc
      841 actgaaatat ataaaaccat attaagtatc aatatgaaaa ttcccgttct ccaacctggc
      901 ttcaactttt ttgcccctgc tggatactct gctgccgttg ctcctaatcg tgctgaaaat
      961 gcctatgcgg attacgtttt ggatataggt aagcgaatac cgctttccgc agcagattta
     1021 agcaacgtat acgaaagtgt aattcgcgcc gtccatgaca gccgtagcag gctcatcgat
     1081 cagcatacgg tcgatatgat tggcaacact gtacttgatg cgttgagccg atcacaaaca
     1141 tttcgtgatg ccgtaagcta tggcattcat aatgaggagg tacacattgg ttgcattaaa
     1201 tacagaaacg aatacgagct taacggagaa tctgctatca aaattgatga tattcaatca
     1261 ctaacctgta acgaattata tggatacgat gtcgggcaag aaccaattct ccccatttgc
     1321 gaggcaggag aaaacgagaa cgaagagcct tatgtcagtt ttagtgttgc gccagatact
     1381 gattcttatg agatgccatc gtggcaggaa ggactgattc acgagattat tcatcatgtt
     1441 actggggcca gcgatccatc tggagatagt aatatagagc taggacccac cgagattctc
     1501 gcacgtcgtg tcgctcaaga gctgggatgg agtgttcccg acttcaaagg atatgcagat
     1561 ccagaacgag aagctcatct tagactacgt aacctgaatg cccttcgaca ggctgccatg
     1621 aggcatgaaa agaatgagag ggctttcttc gaaagactgg gtacgatcag tgaccgatat
     1681 gaggcgagtc ctgatttcac agagtattcc gctgtgtcta acataggata cggatttatc
     1741 cagcaacatg attttccagg ggtggctatc gacgagaatt tacaggatgt aaatcagatc
     1801 caactgtatc atggtgctcc ttatatcttt acatttgggg atgtggacag acacaattag
     1861 cgctgattcg tctttacagt tacatagggt tactactcat agatattaac ggagttgatg
     1921 atgggcaatc gtgcaacatt gtattgtatg taaaggcaga gccccgaatt ttactgttgt
     1981 aagtgggcct ggcgggaatg gtatcgccag catcattggc ttgggtccag taaaaagaga
     2041 cgcagaaaga tgcacaatag gcactacgcg tcatgcatgg attaggtcgc tcatcattta
     2101 cacagtactg ctaaaaaatt attaaggaaa gcgcggcact tcgtatgtaa gaacgtgtcg
     2161 cggttggctg gtgaactttc gatagtgcga gtattgaatg atttccagcc gttaccgatt
     2221 ttacgtgttt attagtgaac aaaccactcg tcagcagact cccaggtatc tttcagagtc
     2281 tcctgaacaa atgtttttgc agaatcttta tctgcggtgc gtgtaacaga aagcccatcg
     2341 ttgctggtgg cttttacgat cacctctaca tcgtcataac gtttactgat gcgtcgggtt
     2401 aattcttcct ttaacgcatc cacagcaccg tttggcattt tgctgatttt ttctttagca
     2461 atacagatct caacacgcat aacaccctcc tgtaactgtg gttatataca ggtgttattt
     2521 ttatctgtat ggataaccag tgtcaattcc tgatattgtt tatggggcat caatggggca
     2581 tgtatgggac actttttatc ggcgaaattc gtcgaagttc gtcgacatgg taaacgaatc
     2641 atctatccaa cccttgaaaa acggcgctcc tggacgatct tcgtcgattt ttaaaaatgt
     2701 tgcgtcatgc agttaaagtg acgggcatac tcttcaaggc tggtgatccc caaccgcacc
     2761 catttaggat gcgaccattg cggtagaccg atgtaaatca taacgcgtga aggatctctt
     2821 ccacgctacg cacacgggcg atgcgcgggt agatatgatt aatgctgtta ttgtgttgct
     2881 cggcgctggc ggcactacag gcgtcttcgg caatcaccag attaaagcca agttcccagg
     2941 cattgcgggc ggtggattca acaccgatat tggtcgagat cccacataac actattgtat
     3001 cgatacctcg gcggcgtaat tgcaactcca gatccgtacc gtaaaacgca ccccactgac
     3061 gtttgatgat ttcgatatca ctgtcagttg cacctaatgc agctggatgt tgccaccagt
     3121 attcgggcaa cactttagcg ggggaggggg catcaaccgg ctgttttaat gcttcggcgt
     3181 aatcggcaga ccagccaact cgcaccagaa acacgggctg accgctggcg cgaaatttcg
     3241 ccgccagctt cccggcgcga ttaactacct catcggcagt atgtggacca ccggcaaaag
     3301 gtaagatgcc ttcttgtaaa tcgatcacca ccagcgcagt ggttttagca ttaagttcaa
     3361 gcataatgac tcctgaaaaa acaaaatcgt gcctcacacc ttaatgcgat tagccgatgg
     3421 cggaacgaca aattttgtta atttttatga gttcgggcaa cacctttata gcaaactggc
     3481 gtagagtctg atttcaggcc cgatataaag tggtaacgag gcgttccagc gcgggccagg
     3541 ctcttacggt gcggcagaat ttccagtata atagccgcct tttttcattc agttgtgaca
     3601 tacagctaac gctgcgactt ggtcacctgc gcagcagggg gcatccgccg gcagctaagt
     3661 taagggatat ctcatgcgta cagaatattg tggacagctc cgtttgtccc acgtggggca
     3721 gcaggtgact ctgtgtggtt gggtcaaccg tcgtcgtgat cttggtagcc tgatcttcat
     3781 cgatatgcgc gaccgcgaag gtatcgtgca ggtatttttc gatccggatc gtgcggacgc
     3841 gttaaagctg gcctctgaac tgcgtaatga gttctgcatt caggtcacgg gcaccgtacg
     3901 tgcgcgtgac gaaaaaaata ttaaccgcga tatggcgacc ggcgaaatcg aagtgctggc
     3961 gtcctcgctg actatcatca accgcgcaga tgttctgccg cttgactcta accacgtcaa
     4021 caccgaagaa gcgcgtctga aataccgcta cctcgacctg cgtcgcccgg aaatggctca
     4081 gcgcctgaaa acccgcgcta aaatcaccag cctggtgcgc cgttttatgg atgaccacgg
     4141 cttcctcgac atcgaaactc cgatgctgac caaagccacg ccggaaggcg cgcgtgacta
     4201 cctggtgcct tctcgtgtgc acaaaggtaa attctacgca ctgccgcaat ccccacagtt
     4261 gttcaaacag ctgctgatga tgtccggctt tgaccgctac tatcagatcg ttaaatgctt
     4321 ccgtgacgaa gacctgcgcg ctgatcgtca gcctgaattt actcagatcg atgtggaaac
     4381 ttctttcatg accgcgccgc aagtgcgtga agtgatggaa gcgctggtgc gccatctgtg
     4441 gctggaagtg aagggcgtgg atctgggcga tttcccggta atgacctttg cggaagccga
     4501 acgccgttat ggttctgata aaccggatct gcgtaacccg atggaactga ccgacgttgc
     4561 tgatctgctg aaatctgttg agtttgcagt atttgcaggt ccggcgaacg atccgaaagg
     4621 ccgtgttgcc gctctgcgcg ttccgggcgg cgcatcgctg acccgtaagc agatcgacga
     4681 atacggtaac ttcgttaaga tctacggtgc gaaaggtctg gcttacatca aagttaacga
     4741 acgcgcgaaa ggtctggaag gtatcaacag cccggtagcg aagttcctta atgcagaaat
     4801 catcgaagcc atcctggatc gtactgccgc gcaagatggc gatatgattt tcttcggtgc
     4861 cgacaacaag aaaattgttg ccgacgcgat gggcgcactg cgcctgaaag tgggtaaaga
     4921 ccttggtctg accgacgaaa gcaaatgggc accgctgtgg gttatcgact tcccgatgtt
     4981 tgaagacgac agtgaaggcg gcctgacggc aatgcaccat ccgttcacct caccgaaaga
     5041 catgacggct gcagagctga aagctgcacc ggaaaatgcg gtggcgaacg cttacgatat
     5101 ggtcatcaat ggttacgaag taggcggtgg ttcagtacgt atccataatg gtgatatgca
     5161 gcagacggtg tttggtattc tgggtatcaa cgaagaggaa cagcgcgaga aattcggctt
     5221 cctgctcgac gccctgaaat acggtactcc gccgcacgca ggtctggcat tcggtcttga
     5281 tcgtctgacc atgctgctga ccggcaccga caatatccgt gacgttatcg ccttcccgaa
     5341 aaccacggcg gcagcgtgtc tgatgactga agcaccgagc tttgctaacc cgactgcact
     5401 ggctgagctg agcattcagg ttgtgaagaa ggctgagaat aactgatatg actcaaatac
     5461 acgaaatcat tcgcgttgca tcgaggcggc aactgagtga actcccatga gcatagataa
     5521 ctatgtgaat gggatgagcg aaggcagtca acgaagaggc agcgtgaagg ataaagtgta
     5581 taagcgtccc gtttcgatct tagtggtcat ctacgcacaa gatacgaaac gggtgctgat
     5641 gttgcagcgg cgtgatgacc ccgatttctg gcagtcggta accggcagcg tggaagaggg
     5701 ggaaaccgcg tcgcaagctg ccatgcgcga agtaaaggaa gaggtcacca ttgatgttgt
     5761 cgctgaacaa ctgaccttaa ttgactgtca gcgcacggta gagtttgaaa ttttttcaca
     5821 tttacgtcat cgctatgcgc cgggcgtgac gcgtaatacg gaatcatggt tctgtcttgc
     5881 gcttccgcac gaacgccagg tagtcttcac tgaacatctg gcttacaagt ggcttgatgc
     5941 gcctgctgcg gcggcgctca ctaagtcctg gagcaaccgg caggcgattg aacagtttgt
     6001 aattaacgct gcctgaacag gcagggctat ctggagatat ttttatggca ggtcatagta
     6061 aatgggccaa caccagacat cgtaaagctg cgcaggatgc taagcgcggt aaaatcttca
     6121 ctaaaatcat tcgtgagctg gtaaccgcgg ctaagctggg cggtggcgat ccggacgcta
     6181 acccgcgtct gcgtgcggcg gtggataaag cactgtctaa caacatgacc cgtgacacgc
     6241 tgaaccgcgc aattgcacgt ggtgtgggcg gtgatgatga tgcgaacatg gaaaccatca
     6301 tctacgaagg ttacggtcct ggcggcacgg caatcatgat tgaatgtctg tctgacaacc
     6361 gcaaccgtac cgttgctgaa gtgcgtcatg cattcagcaa atgtggcggt aacctcggta
     6421 ctgatggttc cgtagcctat ctgttcagca aaaaaggcgt catctccttc gagaaaggcg
     6481 atgaagacac catcatggaa gcagcactgg aagcaggtgc tgaagacgtt gtgacctatg
     6541 atgacggcgc gattgatgtc tacactgcat gggaagaaat gggtaaagtg cgcgacgctc
     6601 tggaagcggc aggtctgaaa gcagacagcg cggaagtttc catgatcccg tctaccaaag
     6661 ctgatatgga tgcagaaacc gcaccgaaac tgatgcgtct gatcgatatg ctggaagatt
     6721 gcgacgacgt gcaggaagtt taccataacg gtgaaatctc tgatgaggtc gcagcgactc
     6781 tctgatgagg cctgctaaac agcaaaacgg agacgcgtga tggctattat tctcggcatt
     6841 gatccgggtt cgcgcgtgac cggctacggc gtcatccgcc aggtaggtag gcaactgtcc
     6901 tacctgggta gcggatgcat ccgcaccaaa gtggatgatt taccgtctcg tctgaaactc
     6961 atctatgcgg gcgtgacgga aatcatcacc cagttccagc ctgattattt cgccattgaa
     7021 caagtcttta tggcaaagaa cgctgactca gccctgaaac tgggccaggc gcgcggcgtg
     7081 gcgattgtgg cggcggtgaa tcaggagttg ccagtatttg aatacgcggc acgtcaggta
     7141 aagcaaacgg tggtaggtat tggtagtgcc gaaaaaagcc aggtgcagca tatggtccgc
     7201 accttgctga aactgcccgc taatccacag gcggatgccg ccgatgcgct ggcgattgcc
     7261 atcacccact gccacgttag tcagaatgcg atgcagatga gcgaatcgcg gctgaacctg
     7321 gcgagagggc gactgcgtta agttataccg cctcggtcag ttccggctga ggcgtttcca
     7381 ctccctccgc atgaatcaac accaaacctg gatgatgcca caaactggct ggcgtgacgg
     7441 ttttacgctc ccacggaata gaacccagga accagaattt ccagaaagtg agttttgcat
     7501 tcggattgct atttaacaat tctccaaacg tctctatttc acccaccgga atacatagcg
     7561 cttctttata aatatcaaaa acaaattttg aacagaactg gcgcgaagat tcgtatttaa
     7621 aaccggtgtg gtaaagtttg cgtagccggg agggaacctg ttcaacaatt cgttgttttt
     7681 gttgttctgt cagcccagcg tctaatcgct ttatagcata gcgttgatta gacgaacgtt
     7741 taataaaacg agatagcgtg gtgatggttg aaaggggaac ccggctttct gcaaccagga
     7801 agtcttcacc gttatgaccg ataatgatcc cgacgtgatt actccagcaa tttgatgcag
     7861 ctgatatttg accaaataag gcagcaccta tacatgtaaa gacgatatct ccaatttcat
     7921 attcagcagg gtaattaata ttcacgttgt cattccattg aaatagatac gaatatacgt
     7981 aatagcataa aaa
//
LOCUS       DAZUGK010000199         7985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZUGK010000199 DAZUGK010000000
VERSION     DAZUGK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.200"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHY5W_003907"
     CDS             <1..746
                     /locus_tag="ACHY5W_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFS4448702.1"
                     /translation="NVTLIKQINDLKMWVNKYIDDCTDEELDDRDFITSVVDRAIFHF
                     AINSICNPEDTKDATPIDRYTFDVETKNGLPSTVQLFYKESKDNEPLANIHFQAIGSG
                     FLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQ
                     RDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNERTLDPDILSKMRDAAPQLISFAKS
                     FDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            1109..2146
                     /gene="dusA"
                     /locus_tag="ACHY5W_003908"
     CDS             1109..2146
                     /gene="dusA"
                     /locus_tag="ACHY5W_003908"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFS4448703.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAIYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2280..2522
                     /gene="pspG"
                     /locus_tag="ACHY5W_003909"
     CDS             2280..2522
                     /gene="pspG"
                     /locus_tag="ACHY5W_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFS4448704.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFIGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2688..3671)
                     /locus_tag="ACHY5W_003910"
     CDS             complement(2688..3671)
                     /locus_tag="ACHY5W_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFS4448705.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQIINYREENLVERVKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3754..5169
                     /gene="dnaB"
                     /locus_tag="ACHY5W_003911"
     CDS             3754..5169
                     /gene="dnaB"
                     /locus_tag="ACHY5W_003911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4448706.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5222..6301
                     /gene="alr"
                     /locus_tag="ACHY5W_003912"
     CDS             5222..6301
                     /gene="alr"
                     /locus_tag="ACHY5W_003912"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4448707.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6554..7747
                     /gene="tyrB"
                     /locus_tag="ACHY5W_003913"
     CDS             6554..7747
                     /gene="tyrB"
                     /locus_tag="ACHY5W_003913"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFS4448708.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1951 a         1891 c         2069 g         2074 t
ORIGIN      
        1 aaaatgtgac gttaattaag caaataaatg atctcaaaat gtgggtcaat aagtatattg
       61 acgattgcac tgatgaggaa ttagacgatc gtgactttat tacttcagtt gttgatcggg
      121 ctatttttca tttcgcgatt aatagtatat gtaatcctga ggatactaaa gatgcgactc
      181 ccatagaccg atatactttt gatgtagaaa ccaagaatgg ccttccctcc acggttcagc
      241 tattttataa ggaatctaag gataatgaac ctttagcgaa tatacatttt caagcaatag
      301 gttctggttt tttaacgttt gttaacgcct gccaggaaca tgatgacaac agcttaaaat
      361 tatttgcttc gctgttaatt tcactttcat attctagtgc ctacgcagat ttatcagaaa
      421 cagtgtatat taatgaaaat aatgagagct acctgaaagc tcagtttgaa aaattatctc
      481 aacgtgatat gaagaagtac ctgggagaga tgaagcgtct ggcagatggg ggagaaatga
      541 attttgatgg ctatctggat aagatgtcac atctggtgaa tgaaagaacg ctcgatcctg
      601 atattttaag caaaatgcga gatgctgcac cacaattaat tagcttcgcg aagtcgtttg
      661 acccaacctc aaaggaagag attaaaatac ttacagacac ttctaaatta atttatgatt
      721 tgttcggggt taaatcggag aaataatatg tgaagttctt cgatagtatg gaaggcatta
      781 tataaaagga cccaatattt attgggttct tttttctcta tcaatgctat tagcagggag
      841 agatatcacc ggagtttaat gtgtgatttt ttatttatcg tcgaacttag attgtttatc
      901 attggcctta acaaagctaa cggctaataa gattatttcc atcacttcgt gagaacttca
      961 tgccttgaga ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt
     1021 atagtacccc gctattgagc ctcctgaaca gtgatgctga ataacataac tcaatgatat
     1081 atcgataaaa taatctctac atttgaaaat gcacggtaat tctgaaatgc aaaaaatcaa
     1141 ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc
     1201 accaatgctc gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa
     1261 tacgttgctg tataccgaaa tggtgactac aggggcgatt attcacggta aaggtgatta
     1321 tctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcggcggta gcgatccggc
     1381 ggcgctggcg cagtgtgcga agctggcaga agcgcgtgga tatgatgaga tcaacctgaa
     1441 tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa
     1501 tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccggtgac
     1561 ggtgaaaacg cgtattggca tcgatgacca ggacagctat gaatttctct gcgatttcat
     1621 caacaccgtt tccggcaaag gcgagtgtga gatgttcatc atccacgcac gtaaagcctg
     1681 gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt atccgcgtgt
     1741 gtatcaactg aagcgtgact ttccgcatct gacgatgtcg attaacggtg gtatcaagtc
     1801 gctggaagag gctaaagcgc atttgcaaca tatggatggc gtaatggtcg ggcgcgaggc
     1861 gtatcagaat ccgggtattc tggcggcggt agaccgagag atctttggtt cctcggatac
     1921 cgatgccgat ccggtggcgg tagtgcgcgc catatatccg tacattgagc gtgaactcag
     1981 ccaggggacg tatctcggcc atattacccg gcatatgctg ggcttgttcc agggtattcc
     2041 tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgcagacat
     2101 taatgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa
     2161 gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt aaatcaatag
     2221 attatttttg gcatgattct tgtaatgcct gcaagagatt tcatatttgg gagagcatca
     2281 tgctggaact actttttgtg attggctttt ttgtcatgtt gatggtcacc ggcgtttcgt
     2341 tgctgggcat tatcgccgcg ctggttgtgg cgacggccat tatgttcatc ggcggtatgc
     2401 tggcattgat gattaagttg ctgccgtggt tactcctggc gattgcggtg gtgtgggtta
     2461 ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact
     2521 aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga
     2581 taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag
     2641 ccgaactata aaaagaaagg gcttcccagg tggaagccct atttctttta tggaatcaac
     2701 aggctggaac cttgcgtcgc gcggctttcc agaatctcat gcgcacgctg cgcatccttc
     2761 agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag
     2821 aacagttcat tactggcctc ggttaattcc tcccgcgtgg tgatatagcc ttgcagggaa
     2881 gggcgtgtca catacaacga gcctttctga ttgagaatgc ctaagttcac accggtaacc
     2941 gcacctgatg agttgccaaa actcaccatt aagccgcggc gttgcaggca atccagcgac
     3001 cgttcccagg tgtctctgcc cacggaatcg tacactacgc gcactttctt accgccggtg
     3061 atctctttca ctcgctcgac cagattctct tcgcgatagt taataatctg ccacgcgccg
     3121 gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgccc
     3181 agggcttttg cccactggca ggcaatcaag ccaacgccgc cagccgctgc gtggaacagg
     3241 aactgttcat cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct
     3301 ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta
     3361 tccgcattaa tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc atagactaca
     3421 cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgccgct
     3481 tcggtaccta atccgctggg tagcgatggc ggcgggtaaa ggccgctgcg gatatacgtg
     3541 tcgataaaat tgatgccaat ggctttattt tcgacctgga tttcattctc cgccggatcg
     3601 gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt
     3661 cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc
     3721 gacccattct ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa
     3781 cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac
     3841 tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga acgctgggat
     3901 gatgtagccg agcgtgtggt agcagacgat ttttacaccc gcccacaccg tcatatcttt
     3961 actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac ccttgcggaa
     4021 tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg
     4081 tcaaaaaata cgccaagtgc ggcgaacatc agtgcctatg cggacattgt gcgtgaacgt
     4141 gccgttgtcc gtgagatgat ctcggttgcg aatgagattg ccgaagccgg ttttgatccg
     4201 caggggcgta ccagcgaaga tctgctggac ctggctgaat cccgcgtctt taaaattgcc
     4261 gaaagccgtg cgaacaaaga cgaagggccg aagaacatcg ccgatgtgct tgacgcaacc
     4321 gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg ggtaaacacc
     4381 ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc
     4441 gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga acctcgtcga aaacgcggcg
     4501 atgttgcagg ataaaccggt acttatcttc tcgctggaga tgccatcaga acagatcatg
     4561 atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac cgggcagctc
     4621 gatgacgaag actgggcgcg catttccggc accatgggta ttttgctcga aaaacgcaat
     4681 atctatatcg atgactcctc cggcctgacg ccaacggaag tgcgttcccg cgcgcgccgt
     4741 attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc
     4801 gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctcccg ctcgctgaaa
     4861 gcactggcga aagaactgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg
     4921 gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag
     4981 caggatgcgg acttgatcat gtttatctat cgtgatgagg tgtatcacga aaacagtgat
     5041 ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggta
     5101 cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac
     5161 gacgaataat aattatttta tgaattaggt aattaaagca aacacttatc aaggaacaca
     5221 aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct
     5281 tcgtgaactg gctcctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca
     5341 cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct
     5401 cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg
     5461 cttttttgat gccagagatc tgccgacgat ttctgcgcaa cattttcata ccgccgtgca
     5521 taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg
     5581 gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt
     5641 ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt
     5701 tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa caactcgcta tctttaatac
     5761 cttttgcgaa ggcaaaccag gtcaacgttc cattgccgcg tcgggtggca ttctgctgtg
     5821 gccacagtcg cattttgact gggtgcgccc gggcatcatt ctttatggcg tctcgccgct
     5881 ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca gtgatgtcac taacctccag
     5941 cctgattgcc gtgcgtgagc ataaagtcgg agagcctgtc ggttatggtg gaacctgggt
     6001 aagcgaacgt gatacccgcc ttggcgtagt cgcgatgggc tatggcgatg gttatccgcg
     6061 cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc gaagtgccga ttgtcgggcg
     6121 cgtggcgatg gatatgatct gcgtagactt aggtccacag gcacaggata aagccgggga
     6181 cccggtcatt ttgtggggtg aaggtttgcc cgtagaacgt atcgctgaaa tgacgaaagt
     6241 aagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta
     6301 atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccaa
     6361 taaaatccta ccaattcaat aagttgatat tctttcatgc tcttataaag gtcgtacctc
     6421 tggcggatgt acgtttgtca tgagtctcac tctgttgcta attgccgttc gcccctgaac
     6481 atccactcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct
     6541 ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct
     6601 tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagta tcggtctgta
     6661 ctacaacgaa gacggaatta ttccacaatt gaaagccgtg gctgatgcag aagcgcgcct
     6721 gaatgcggtg cctcatggcg cttcgcttta tttaccgatg gaagggctta acagctatcg
     6781 ccatgccatt gcgccgctgc tgtttggtgc cgaccatccg gtactgcaac aacagcgcgt
     6841 agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggtgcgg atttcctgaa
     6901 acgctacttt ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc
     6961 aatattcgcc ggggctggat tcgaagtaag tacttacccc tggtatgacg aagcgactaa
     7021 cggcgtgcgc tttaatgacc tgttggcgat gctgaaaaca ttacctgccc gcagtattgt
     7081 gttgctgcat ccatgttgcc acaacccaac gggtgccgat ctcactaatg accagtggga
     7141 tgcggtgatt gaaattctca aagcccgcga gcttatccca ttccttgata ttgcctatca
     7201 aggatttggt gccggtatgg aagaggatgc ctacgccatt cgcgccattg ccagcgctgg
     7261 attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt
     7321 cggcggactt tctgttctgt gtgaagatgc cgaagctgca ggccgcgtac tggggcaatt
     7381 gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc
     7441 tgcagtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg
     7501 tactcgcatt ctggcaatgc gtcaggaact ggtgaaggta ttaagcacag agatgccaga
     7561 acgcaatttc gattatctgc ttaatcagcg cggcatgttc agttataccg gtttaagtgc
     7621 cgctcaggtt gaccgactac gtgaagaatt tggtgtctat ctcatcgcca gcggtcgcat
     7681 gtgtgtcgcc gggttaaata cggcaaatgt gcaacgtgtg gcaaaggcgt ttgctgcggt
     7741 gatgtaatgc agtaaagcag gctggaatta cccagcctgc agtgaaatta aactgtcgtc
     7801 gctttcacac tttctttata gatgattttt ttgataccat cgttctacgt gagagataat
     7861 aaacgttgtt agttctttta ttgttaagct tatcctaatt atctggaatt ccttaccctg
     7921 ttttttgtgt ggggtaataa tttctgcatt tctaatcatt attaaactta ataataagtg
     7981 ccggg
//
LOCUS       DAZUGK010000200         7969 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZUGK010000200 DAZUGK010000000
VERSION     DAZUGK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.201"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2095)
                     /gene="tssI"
                     /locus_tag="ACHY5W_003914"
     CDS             complement(<1..2095)
                     /gene="tssI"
                     /locus_tag="ACHY5W_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFS4448709.1"
                     /translation="MSQAGLRFTVDVDGQPTETFAVTRFHLSQSYFSLFTLEINTVSE
                     RTTLKASTLLEQTAQLTIWQGSTPLRYVTGLIAGFERGEYDGRKTSYSLTVRPPLWRS
                     SLRQNFRHFQQQDIQSIIQRLLDDNRVGDTAYLFDEMHPAREFCVQYGETDYEFLCRL
                     IAEEGIFFYEEHAQKGSRQKLVFCDRVVCLPEAFPVPFNPQPRGTFTECINRFRYSAQ
                     IRPAYVITKDYSFKAPHWQARYRNDAPGLNGQRNQYEKLDYPGRFKDEQHGKDFVRYQ
                     TEGWRNDAETASGSSNSPKLWPGARFTLTEHPADDLNRDWQVVASELHGEQPQASPGT
                     HKEGTTLVNEFQAIPADKTWRSSPLPKPKVDGPQPAVVTGPEGEEIFCDEHGRVRVKF
                     LWDRYHKANEDSSCWIRVSQPWAGAGFGHLAVPRVGQEVIVDFLNGDPDQPIIVGRTY
                     HHDNRSPGNLPLTRTQTTIRSKTYKGNGFNELRFEDMTGQEEVYIHAQKDMNHVINHD
                     RTSLTKRHSVDKVQGNSAVLVDESQVMQVGGTQNTSIGGDYVLSVGNHFTDGYRKMNL
                     AAFQDDAATLLKQSAFLVGANGIMPGMGNMTTFVQGSRTEHINLTDTSSVIGAKLINA
                     GEMISISAGTLLSLTSGENSKETCGGVKIIQAVDGIELHTGMASLTLRNNGHIEIKGI
                     KVTIEGSDDVTVKTQA"
     gene            2400..4259
                     /gene="tssF"
                     /locus_tag="ACHY5W_003915"
     CDS             2400..4259
                     /gene="tssF"
                     /locus_tag="ACHY5W_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS4448710.1"
                     /translation="MTSGEDYYREELDYIRRYARLLARENPDLKNFLGDKDVDPDTER
                     LMGTFAFLSGRLREKIEDRFPEITVPLIKRLCPSYLRTVPPMTIIEYTPDNTLATVYD
                     IPRDAQVMNAPHRDSHPEESYLIPGAQDDLPEEEEPPCIFTLCRNIRLLPLHIHNVNN
                     CSTPDQGIIDIIFAPLPGSRITAADLKGISLWLGDENIISRWQVYLWLCRYRRGAELI
                     TGGRRYPQPGLTIKPSGFSHDESLLPQPGSLHSGYRIIQDWFCFPDAFFFFDLSGISL
                     PGEIISRPFTLRLRFDRPLPETVNLSRQSLRLHCTPAVNLFFHDAVPVTPDNPHQEYP
                     LQASQFWPGYFDIFRVDSVQGQEHNTFRDDDLVALSRHGNQRKWLSSDSPLRPMEYHQ
                     ERRIIYWKHSVRRSLLSVNPAHFISFRHADNSVPDATLTGRDPIQISLICTNGDQPCR
                     LAPGDICVPVGQDASVAPFRNVTTPTPPLPPVPDGPRHWSLLNTMTLNYLTMNDVEVL
                     RDILRTFDRCGIHTPFCARLSPEKLNALEKLETRPTDRVFRDIVVRGLSSTLYVNPQP
                     FACEGEMYHLGTVLSHFFALYASVNSWHMLTMINTQTEEVWRWTERTGQFPAM"
     gene            4223..5284
                     /gene="tssG"
                     /locus_tag="ACHY5W_003916"
     CDS             4223..5284
                     /gene="tssG"
                     /locus_tag="ACHY5W_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS4448711.1"
                     /translation="MDRENRTVSGDVTPADVTTNQDETQALRDACYGNFFVVTDLLYR
                     SRKDAVSLRTEPEQENVHFRASASIAFPLRDIESLEKNAAGQYVMTTHFMGLSGSSSP
                     LPGYYLDEMAWQQAQEGSSATADFLNLFSHRWTQFRYHIWQKYRWHVNFRNDGSDAIS
                     QSIYSLIGLGNPRVRERLNICHSKLLAYAGILAGTGRSPEIVCNLIEHCFDLSDVKLE
                     NWQLRRVPIPQEQQNRLGVSELTGEGGLQGKSVLGKNFIIGRHIPDLSGKFLLCINNL
                     KLERYLAFLPEGEDYQPLTTFVSFLLRDQFAWDLRLGLAPDEISGMRLGDRSTSQLGR
                     TMFIGKPRRENFISIRVRG"
     gene            5620..6081
                     /locus_tag="ACHY5W_003917"
     CDS             5620..6081
                     /locus_tag="ACHY5W_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448712.1"
                     /translation="MATSPSPDCDLLSIPFSAATDFTILTAHGDRFAQTLIECHNPAL
                     KMALCGRLNTCLALLRPTLNEPIPPHLIDGFITNDVPDVSPGFEPDAESLCDYCLALT
                     QILNGHSLLPETETTLTGLLCELVWYFAADLNAPHWIRTADGVQVIDGRPA"
     gene            6078..6332
                     /locus_tag="ACHY5W_003918"
     CDS             6078..6332
                     /locus_tag="ACHY5W_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448713.1"
                     /translation="MMQQDWHPADIIAGLRKQRTSLAEVSRSAGLASATLANALSRSW
                     PRGEKLIADVLETSPEIICPSRYFDDQGNRTERPMRKRYS"
     gene            complement(6356..6490)
                     /locus_tag="ACHY5W_003919"
     CDS             complement(6356..6490)
                     /locus_tag="ACHY5W_003919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448714.1"
                     /translation="MRIKTTPAVTFTSSGMVSCRASLVFSRLSASKTLVESRESPILL
                     "
     gene            6453..6755
                     /locus_tag="ACHY5W_003920"
     CDS             6453..6755
                     /locus_tag="ACHY5W_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS4448715.1"
                     /translation="MVKVTAGVVFMRMFNPHHPGAVLRDYLGGTSIVQAASALNISRS
                     RLSRILNGHAPVTADMALRLAALLNTRAELWAGMQSEYDLWQASQKPRPVIRSLRG"
     gene            complement(7185..7325)
                     /locus_tag="ACHY5W_003921"
     CDS             complement(7185..7325)
                     /locus_tag="ACHY5W_003921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448716.1"
                     /translation="MAQARTGAACGGELCGVTLPCRWRDQSDALSGAGGKRCASGAVF
                     KN"
     gene            7565..7969
                     /locus_tag="ACHY5W_003922"
                     /pseudo
     CDS             7565..7969
                     /locus_tag="ACHY5W_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587348.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
BASE COUNT         1886 a         2116 c         1996 g         1971 t
ORIGIN      
        1 gagcctgtgt ttttactgtc acatcatcac tgccttctat cgtaactttt attcctttga
       61 tctctatatg accattgttt cttaatgtca aagaggccat tcccgtatgt aattctattc
      121 catcaactgc ctggattatt tttacgccac cacatgtttc cttgctattc tctccagaag
      181 tcagactcag taatgttccg gcactgattg aaatcatttc tccggcattg atcagttttg
      241 caccaattac tgaactggtg tctgtcagat taatatgttc cgttctactt ccctgtacaa
      301 aagtggtcat gtttcccata ccaggcatga tgccgttagc accgactaaa aatgccgatt
      361 gtttcaacag ggtggcggca tcatcctgga aagctgccag attcatttta cggtagccat
      421 ccgtaaaatg attacctacc gacaaaacat agtctccgcc aatactggta ttttgagtgc
      481 caccaacctg catcacctgg ctttcatcca ccaggacagc actgttaccc tgtaccttgt
      541 ccacagaatg ccgtttggtc aggctggtgc gatcatggtt gataacatga ttcatatcct
      601 tctgcgcatg aatataaacc tcttcctgcc cggtcatatc ttcaaaccgc agttcattaa
      661 acccgttccc tttatatgtt tttgagcgaa tcgttgtttg tgttctggtt agtggcaggt
      721 ttcctggcga gcggttatcg tgatgatagg ttcgcccgac gataatcggc tgatccggat
      781 caccgttcag gaagtccacg atcacctcct gacccactcg tgggacagcc agatgaccaa
      841 aacccgcacc agcccacggc tgagacaccc gtatccagca tgaactgtct tcattcgctt
      901 tgtgatagcg atcccacaaa aatttaactc ttacccgacc atgttcgtcg caaaaaattt
      961 cctccccctc tggtccggtg actaccgctg gctgcggacc atccactttc ggtttgggta
     1021 gcggtgaaga gcgccaggtt ttatctgcgg gtatcgcctg aaattcattt accagcgtgg
     1081 taccttcttt atgtgttccc ggtgaggcct gcggttgttc gccatgcagt tcacttgcca
     1141 ctacctgcca gtcccggttc agatcatcgg cagggtgctc agtcagtgta aaacgtgctc
     1201 ccggccacag ctttggcgaa ttactgctcc cagatgccgt ttcagcatcg ttgcgccatc
     1261 cctctgtctg atagcggaca aaatcctttc catgttgttc atccttgaaa cggcccgggt
     1321 aatccagctt ttcatactgg ttgcgctgcc cgttgagacc gggtgcatca ttacggtaac
     1381 gggcctgcca gtggggggct ttaaaggagt aatctttggt gatgacataa gccgggcgga
     1441 tctgtgcgct gtaacgaaag cggttgatac actcggtgaa tgtgccgcgg ggctgcggat
     1501 tgaaggggac aggaaatgcc tccggcaggc agaccacgcg atcacaaaat accagctttt
     1561 gccgggagcc tttttgggca tgttcctcat aaaaaaagat gccttcctct gcaatcagtc
     1621 ggcagagaaa ctcatagtcg gtttcgccgt actgcacgca aaactcccgc gcaggatgca
     1681 tttcatcgaa caggtatgct gtatctccca cccggttatc atcaagcagc cgctgaataa
     1741 tgctctggat atcctgctgc tgaaaatgac gaaaattctg ccgtaggctg ctgcgccaga
     1801 gcggggggcg aaccgtcagg ctgtagctgg tttttcgccc gtcatattca ccgcgttcaa
     1861 atccggcgat cagtcctgtc acatagcgta acggtgtgct gccctgccag atagttaatt
     1921 gtgccgtctg ttccagtagt gtactggctt tcagggtggt ccgttcactg accgtattaa
     1981 tttccagcgt aaacagagag aagtaggatt gcgagaggtg aaagcgagtg acggcaaagg
     2041 tttcagtggg ctgaccatcc acatcaacag taaaccgaag acctgcctgt gacataaata
     2101 taccctcaac aaaaatttcg ccaggggaaa ccaaatgtca gtgtatttgt gagaatgtaa
     2161 tttgtttgtt acttggctga cttatttgtt atcaccagac gttaggttaa ttaatggaat
     2221 catcctccac taaagcgtat tgtcaagcca taaatttaca gattaatcat tccgtaaaaa
     2281 taataccgga acagttcatc cgttcttctg actctgtgat aacctgttgt ggctgaaata
     2341 ttatttctca ggtacagaat cagataatat aaaccacata cattcactgg agttatatta
     2401 tgacatctgg agaagattac taccgggaag agctggacta tattcgcagg tacgccagat
     2461 tactagcgcg ggaaaaccct gacctgaaaa actttctcgg tgataaagat gtagacccgg
     2521 atacagaacg actgatggga acatttgcct ttctttccgg cagactacgc gaaaagattg
     2581 aagaccggtt tcccgaaatc accgtgccat taattaaacg tttgtgtccc agttatctgc
     2641 gcacggttcc ccccatgacg attatcgaat atacgcctga taataccctt gccacagtgt
     2701 acgatattcc ccgcgatgca caggtgatga atgcccccca cagggatagt caccctgaag
     2761 aaagttatct gatacccggc gcacaggatg atctgccgga agaagaagag ccgccgtgta
     2821 tttttaccct gtgccgtaat atcaggttgc tgccattaca tattcataac gtaaataatt
     2881 gcagtacacc tgatcagggc atcattgata tcatctttgc gccactgcca ggcagcagaa
     2941 taacggcagc cgatctcaaa ggcatcagtt tatggctggg agatgaaaac attatcagcc
     3001 gctggcaggt ttatttgtgg ctctgtcgct accggagagg cgcggaactg attaccggtg
     3061 gacgacgtta ccctcagcca gggctgacga taaagccttc cgggttcagt catgatgaat
     3121 cattgctgcc acaacccggc agccttcaca gtggctaccg gataattcag gactggtttt
     3181 gttttcctga cgcattcttc ttttttgatc tcagcggaat atcgctgccc ggagagatta
     3241 tctcccgccc ctttacgctg cgccttcgct ttgatcgacc attgccggaa actgttaatc
     3301 tgagcaggca atccctgcgt ctgcactgta ctccggcggt gaacctgttt tttcatgacg
     3361 ccgttcctgt tacgcccgat aacccgcatc aggaatatcc gttgcaggcc agccaattct
     3421 ggcccggtta ttttgatatt tttcgtgttg acagcgtaca ggggcaggag cacaacacat
     3481 tcagggatga tgatctggtc gcgctctccc gccatggaaa tcagcgcaaa tggctgtcat
     3541 cagacagtcc cctgcgcccc atggaatatc accaggagcg ccgaattatt tactggaagc
     3601 acagcgttcg tcgttcgctg cttagtgtca atccggctca ttttatttca ttccgccatg
     3661 ctgacaacag tgtaccggac gccactctca cggggcggga tcccattcag atatcactca
     3721 tctgtacgaa cggcgatcag ccctgtcgcc tggcacccgg tgatatctgc gttcccgtgg
     3781 ggcaggatgc atccgtggcc ccttttcgta acgtcaccac gccaacccca cctttaccgc
     3841 cagtgccgga cggcccgcgc cactggtcgc tactcaacac tatgacgctc aattatctga
     3901 ccatgaatga tgtggaggta ttgcgcgata ttttgcgcac tttcgaccgc tgcggtattc
     3961 atacaccatt ctgcgcccgg ctgtcgccgg aaaaactgaa tgcgctggaa aaactggaaa
     4021 cccggcccac agatcgtgtc ttccgggaca tagtggtacg cggattgtca tccacgctgt
     4081 atgtcaaccc gcaacctttc gcctgtgaag gggagatgta tcacctggga accgtgttgt
     4141 cgcatttttt cgccctgtac gccagtgtca attcctggca tatgctgacc atgattaata
     4201 cccaaaccga ggaggtctgg cgatggacag agagaaccgg acagtttccg gcgatgtaac
     4261 ccccgcagac gtcacaacaa accaggatga aacgcaggcg ttgcgcgatg cttgttatgg
     4321 taactttttt gtggtcactg acctgctgta ccgtagccgg aaagatgccg tttcactgag
     4381 aacggaacct gaacaggaaa atgttcattt tcgtgccagt gccagcattg catttcccct
     4441 gcgggatatt gaatcgctgg aaaaaaatgc agccggtcaa tacgtgatga ccactcattt
     4501 tatgggatta tcaggcagtt catcgccact accgggttac tacctggatg agatggcctg
     4561 gcagcaggca caggagggaa gcagcgcaac agcggatttt ctgaatctgt tcagccatcg
     4621 ctggactcag tttcgctatc acatctggca aaaataccgc tggcatgtca acttccgtaa
     4681 cgatggctct gatgccatat cgcagagcat atattcactt atcgggctgg gtaatccgcg
     4741 ggttcgtgag cgacttaata tctgccacag taaactgctg gcatatgccg gcattcttgc
     4801 cggaacaggg cgctcgccgg aaattgtctg taaccttatt gagcactgtt ttgatctgtc
     4861 agatgtgaaa ctggagaact ggcaattgcg ccgggtgcca atcccgcagg aacaacagaa
     4921 ccggctgggg gtcagtgaac tgaccgggga agggggattg cagggcaaat cggtgctggg
     4981 aaaaaacttc attatcggca gacatatccc ggatctcagt ggtaaatttt tgttgtgcat
     5041 taataacctg aaactggaac gttacctggc ttttttgccg gaaggtgaag attatcagcc
     5101 tttgaccacg tttgtatcat tcctgttgcg tgatcagttc gcctgggatt tgagactggg
     5161 gcttgcaccg gacgagatta gcggaatgcg tctgggggac aggagcacct ctcaactggg
     5221 gcgaacaatg tttattggca aaccacgccg tgagaatttt atttcaatac gggtaagggg
     5281 ataataaata acaggaacgg cgctctttct ggcgaggtac tggccccggt aagtctgctc
     5341 cgggatatcc atatgccctc atgtgactca ttgctaatca cagtatcctt agcctgcgtc
     5401 tgcaaaatca ggcgtcggga ttggaacccc ggattattca tactggcgac acattttttg
     5461 tgtcgtgcgc ttggctgtac ctcaatggtg ggctgggtga gggcactgca aagtgcgccg
     5521 gttccagtat gatcggtagt tccaacctcg ctcagtccac cacccaggag attggaacct
     5581 ctgatggtgg atttaaaacc acatactgga gattgtccaa tggctacaag tccttcccct
     5641 gactgcgatt tactctctat tcctttcagc gccgctaccg atttcaccat tctgaccgct
     5701 cacggcgatc gattcgccca gaccctaatc gaatgccaca atcctgcgct aaaaatggcg
     5761 ctctgtggca gacttaatac ctgccttgca ctgttgcggc ccacgctgaa cgagccgatc
     5821 ccgccgcatc tgatcgacgg ttttattacg aatgatgttc ccgatgtttc acccggtttt
     5881 gaacctgatg cagaatcact ctgcgattac tgtctggcgc tgacgcagat actgaatggt
     5941 cactcactgt taccggaaac cgaaacgacg ctgaccggac tgctgtgcga gctggtctgg
     6001 tattttgccg ccgatctgaa cgccccgcac tggatacgta ccgctgacgg cgtacaggtt
     6061 atcgacggga ggccagcatg atgcagcaag actggcaccc cgccgatatt atcgcgggac
     6121 tgagaaaaca gaggacatcg ctggctgagg tttcccgcag cgcgggactg gcgtccgcca
     6181 cgctggcaaa cgcgctgagc cgttcgtggc cgcgcggcga aaaactcatt gcggatgtgc
     6241 tggaaacatc accggaaatc atctgtccgt ccagatactt cgatgatcag ggcaaccgca
     6301 ctgagagacc catgcgaaag cggtactcct gataacagaa cccgcaagag caccgttaca
     6361 gtaaaatcgg actctcccgg ctttccacca gcgttttgct ggcggaaagc cgggagaaga
     6421 caagcgaagc gcggcaggat accatcccac tgctggtaaa ggtcacagcc ggagtcgttt
     6481 ttatgcgcat gtttaatccg catcatccgg gagcggttct tcgtgattat ctgggcggca
     6541 caagtattgt gcaggcagct tctgcgctga acatttcccg ctcccggtta tcccgcattc
     6601 tcaatggaca tgcgccagtg acggctgata tggcgctgcg tctggcggcg ctgctgaata
     6661 cccgcgctga gctatgggcg gggatgcagt ctgaatatga tctctggcag gcatcgcaaa
     6721 agccacggcc tgttatccgg tcattgagag gttagctcag catttcacgt tcacaaatac
     6781 acgttaaaat caaggggaaa ccctttttcc agaaaaatga atttcccctt tctgcgccag
     6841 aaataacggt gtaatctccc tcctgcacta tgccttcggg caccctttgg cttcatggtc
     6901 tgcaagaagc ccctgattta acaggacgac cagcccttgc taccggtacg acctccgcaa
     6961 taccggaacg cgaaagtgtt tgaggcgaag tacatatata caaggacagc tccatcgtca
     7021 tcgctttacc gcaatggcct tcaccggctg aacggctttc ctttgccgga atttctccgg
     7081 cctacttatc ccttgctgat gaccagcgcc ttttacatga cgggctgcgg cctgcaaaga
     7141 cagcaggctt tgctgccgcg tgaccgttaa tctgcaccag actgttaatt tttgaatact
     7201 gcaccagaag cgcaacgttt cccccctgca ccagacaagg cgtcactttg gtcgcgccag
     7261 cgacagggca aagtgacgcc gcacaactca ccgccgcaag ccgcgccagt cctggcctgc
     7321 gccatatttc cgccgtctga catgcccttt ggggcagctg tcagctcaga gtaaaggaga
     7381 aaaagatcga taccgagggg ggcacgatgc tccggagaca cccctgcttc gtcaggcagt
     7441 ctgtaccgcg tacagcctga aactccttaa acctcaacaa ccagccactc cggcgtgaag
     7501 gtatcgttct ggcgcagcca aacagcacac gccggagact gtttgtttat gttgcagggg
     7561 attcatgagt aaattaagca gtaaaatgaa ggcgctggcg aagaaagcgg gcggcagctt
     7621 caaaacggtg aatgaccata tccatattac aaacggttca gtgaacattt acgggcgctg
     7681 aatatccaga tccagcgggt gcagcagatc aaggttcgcc atatcgaatg ctacattgag
     7741 gaacggctgg agcaggatat tggtttacga acgctgcaaa acggaatggc agcgttgcgg
     7801 accgtgctga gataggctga acgcaggcag gtggtggagc atccgcgtct gaccaataag
     7861 gcattagggg tgtcaggcgc ttcgcgcaac ggcacgcgcc gcgccatcac gccggaacat
     7921 taccagcagg tgatggagaa agcccgcact caggacgcag ggctggcgg
//
LOCUS       DAZUGK010000201         7902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZUGK010000201 DAZUGK010000000
VERSION     DAZUGK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.202"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..307)
                     /locus_tag="ACHY5W_003923"
     CDS             complement(77..307)
                     /locus_tag="ACHY5W_003923"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFS4448717.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            230..722
                     /locus_tag="ACHY5W_003924"
                     /pseudo
     CDS             230..722
                     /locus_tag="ACHY5W_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(789..917)
                     /locus_tag="ACHY5W_003925"
     CDS             complement(789..917)
                     /locus_tag="ACHY5W_003925"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFS4448718.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPE"
     gene            complement(948..1714)
                     /locus_tag="ACHY5W_003926"
                     /pseudo
     CDS             complement(948..1714)
                     /locus_tag="ACHY5W_003926"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1750..2289)
                     /locus_tag="ACHY5W_003927"
     CDS             complement(1750..2289)
                     /locus_tag="ACHY5W_003927"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4448719.1"
                     /translation="MLTTSESQFRQKDQQLSLVEETEAFLCSALARAEEKIEEDEREI
                     EHLQTQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDAPQVPRQLRQ
                     PRHRRPLPAHLPHAIHRLESEESCCPECGGELDYLEEVSAEQLELVSSALKVIRTVRV
                     KKPVQNVTASLKHRRRPAR"
     gene            complement(2301..3453)
                     /locus_tag="ACHY5W_003928"
                     /pseudo
     CDS             complement(2301..3453)
                     /locus_tag="ACHY5W_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            complement(3434..3910)
                     /locus_tag="ACHY5W_003929"
     CDS             complement(3434..3910)
                     /locus_tag="ACHY5W_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4448720.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            3997..4111
                     /locus_tag="ACHY5W_003930"
                     /pseudo
     CDS             3997..4111
                     /locus_tag="ACHY5W_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4162..5671)
                     /locus_tag="ACHY5W_003931"
                     /pseudo
     CDS             complement(4162..5671)
                     /locus_tag="ACHY5W_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6291..7306)
                     /locus_tag="ACHY5W_003932"
                     /pseudo
     CDS             complement(6291..7306)
                     /locus_tag="ACHY5W_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1836 a         2093 c         1871 g         2102 t
ORIGIN      
        1 gattggtgac aacctcaaat gtattttgag gttgttatat aaaacacatt attttatatg
       61 atttggtgat atccaattag tagaacatgt atttttcgat aaagtaccgg tgttttttta
      121 tgaaatttac agagtgcggg aaacagatag ccctacgggt cagatgtttg attaatattc
      181 tcatgcactc aatatgctga ataaaaatag atgagtctcc ttctataaca tatctgatgt
      241 gactacccca ttcgtcagtg gtcaatatac caatgaaaaa aagagggaag cagcgccagc
      301 aattcacaac agtataggta ttgctgctgc agcccgtgag ctcagcctgt atgaatcaca
      361 gtctacaact gggtagtcaa caacaaaatc agtagacgtt cttctgaacg tgagctggag
      421 atgtctaccg atgttgcacg tctcaaacgc cagctggcgg aacgggatga agagctgacc
      481 atcctccaaa aggccgcgac atacttcgcg aagcgcctga aatgaagtat gtctttattg
      541 aaaaacatca ggctgtgttc aacatcaagg caatgtgcca tgtgctccag gaaggccgca
      601 gtgactggta tatgtggtgt cagcggcgga caaggataag tacatgtcag cagttccgtc
      661 agcactgcga cagcgtggta ctcgcggctt ttatccggtc aaaacagcgc tacggagcac
      721 cgtattgacg cttatttatt ggtgagtact acgttccatg gcaggagttc gtcaacacgg
      781 ttggagggct attccggtag tacgctcaga atatggcgca gatacgcttc cggatcgata
      841 ccgttcagac ggcaggtgcc gatcagcccg tacagcagtg ctccacgctc gccgccgtga
      901 tcgctaccga agaacatgta attctttttc ccgagacaga cggcacgaag cgctctttcc
      961 gctgtgttat tgtccgcctc cgccagaccg tcatcactgt aataacagag ggcgtcccac
     1021 tgattcagta catagctgaa cgcttcgcct ggtctggatt ttttcgacag cgtgccattc
     1081 ttctccacca tccattcatg cagcgacgtc agtaacgctt tgcttcgctg ctgcctgact
     1141 tcaagacgct ctgactctgg taatccccgt atttcatcct cgatggcgta cagttcactg
     1201 atgcgcttca gggcttcttc tgccgtcgca cttttgctgc tgatgtatac atcgtggatt
     1261 tttctccgga catgggctca gcacgcaact tctgtcagcg caccatcttc acgttctgca
     1321 ctgaacagcc gatcgtaacc gttgaacgca tccgcctgca gggggcggag gtgttgctcc
     1381 gggtgtttcc cctgccggtt cggtgagtac gcgaaccaga ccgccggagg agatgacgaa
     1441 cccgcattgc gatcattccg gacatacgtc cagatgcgcc ctgttttcgt cttttcaggc
     1501 ccagtgccag tacctttacc ggtgtgtcgt cagtgtgaag cttgcgggta ttcatcacat
     1561 aacggtacag ggcatcattc agcggagtca ttaactggca gcacgcgtca acccagttgg
     1621 agagtaatgc acggctcagt tcgacaccct gtcgggcaaa gatttcactc tgacgataca
     1681 gtggcagctg ttcgcagtat tttcccgtta acacgcgggc aagtaatccg gggcccgcga
     1741 taccacgctc tatcgggcgg gacggcgccg gtgcttcaac gatgcagtca cattttgtac
     1801 aggctttttt acccgaacag tgcggatcac tttcagggcg ctgctcacca gttccagttg
     1861 ttctgcactg acttcctcca gataatccag ctcaccgcca cactccgggc aacagctttc
     1921 ttcagactcc agacggtgta ttgcatgggg aaggtgtgcc ggtaacggac gacgatggcg
     1981 cggctgtctc aactggcggg gaacctgtgg agcgtcttcc cgcccactgt aacgatcgct
     2041 gtcctgttcg cgttgtttca gcagggcctc agcctgttca acttcacgac gcagtttttc
     2101 agaacgggta ccgaacagca tccggcgcag tttttctatc tgagtctgca gatgttctat
     2161 ttcccgttca tcttcttcga tcttttcttc ggcacgtgcc agtgcagagc acaggaaggc
     2221 ctccgtctct tcaaccagac ttagttgctg gtctttctga cggaactggc tttctgaagt
     2281 ggtcaacaaa aactggccac ctagttagag tttttccagt atcgattttc cgattcgttt
     2341 gggggtaacc caccgttata ttcgtgcggt cttagtgcgc tgtaatatcc aacgatatag
     2401 tccgttatgg cgtgagctgc ctcgctgaag cttacgtaac ccaccaccgg catccattcg
     2461 ttctcttcag actcctgaag aagcgttcca ttgggctgtt atcccagcag tttccgcgcc
     2521 ggctcatact ctgtctgatc tggtatcgcc acaataactg ccggaactgc ctgctcgtat
     2581 aatgactgcc ctgatcgctg tggaacatca ccccgccggg cttaccacgg gtttcccatg
     2641 ccatttcccg cgctttcatg gtgagcctgc tgtccggcga gaacgacatg gcccagccca
     2701 ctggttttct tgcgaacagg tcgagaacaa cggcgaggta cgcccagcgc ttacccgtcc
     2761 agatataggt cacatcaccg caccacacct gatttggctc ggtcacggcg aactgccttt
     2821 caaggtagtt agggatagca acatgttcat gaccaccacg tttataccgg tgagtcggct
     2881 gctgacagct gaccagcccc agctctttca tgagcctgcc agcaagccag cgtcccatct
     2941 ggtagcctct ccgggttgcc attgtggcga tgcttcttgc tccggccgaa ccgtggctga
     3001 tgccatgtag ctcaagtacc tgactgcgta atacagcccg tctgccgtct ggtttttcag
     3061 gacggttttt ccagtatctg tagctgctgc gatgaacccc gaacacatgg cagagtgtga
     3121 ccacaggata atgcgctctg agtttcccga ttatcgagaa ctgttcaggg agtctgacat
     3181 caagagcgcg gtagcctttt ttaatatttc attctccatt tcaatgcgtt gtagcttttt
     3241 cctcagctta cgtatttcga tttgttctgg tgttatcgga gaggcttttg gtgttttgcc
     3301 ctgacgctca tcacgcagtt gtttgaccca tcttgtcatt gtagaaaggc caacatccat
     3361 agctttggcg gcatctgcca ccgtgtattt ctggtcaaca accagttgag cggattcgcg
     3421 tttaaactct gcgctaaaat ttcttttttt cattagagca cctgtgttgt tctgaggtga
     3481 gcatatcacc tctgttcagg tggccaaatt cagtgtgcca cttcacagtg agcagctctg
     3541 gtgaaggatt ttccagcgtc atgttaccgt gacggaactc cactttgcag gagctggcac
     3601 tgacagtagt ctgagtggat aaggatggag taagagcagc catcggttct ttcggctcat
     3661 caggcgttat ctctacaggt aataattcaa cgcctgcgtc agaagtggtt gtcaccggaa
     3721 gacgccgtga tatacgccct tcgttttgcc agagtctgag ccatttgaaa ataacatcat
     3781 cattgacgcc attttctcgt gcaatctggg ccatacaagc accgggttgt gatgccagtt
     3841 caaccatacg aagtttgaat tcattcgaat actttttacg aggttctttt cgccagtcct
     3901 gtaattccat acttggatgt ccgtctgtgt cagatgggcg tctaagttac caattcttgt
     3961 ctaatggcta catactgcgg tcggtttacg cttactttta acgtaaaaac cgtgatggca
     4021 agcctgtgcc gtcacggact gagggcaaag gcttcccgga agttcagccc ttaccgcaca
     4081 cagcctgcct gtgtcagaaa atctgctgga gctgtcaacg acggatgaaa agtgcctctt
     4141 ccgcaacctg ggtggagatt ttcatgccag tggactcatt accctctgcc tgagtagttc
     4201 aaatcctgct cgaccataca tctggcgttt cagcatcttc aggcgattta catgaccttc
     4261 gacaacacca ttactccagc gactactgat tgcctcacat atagctgcag catcagcttc
     4321 catccccgcc gccactcgcc gaaattctgc tgaaccgctt tcgtggactc gagtgaacca
     4381 actacttagc tgtgatttat tttgggtttt cagaatacgg tagaactcga gtgccagttg
     4441 ctgcgctatt ttcagctcag gttcttttca cacatcagac taataaatcg ggaagcataa
     4501 ttttcttcat ccctggttat tctccagggc atcaaccagc ggcttacccg ggccaccatt
     4561 tcccgccata tccggctggc gttataatta ccactttctc gttgctcttt taaccactcc
     4621 ctccatgggt ccagaagccc tcgctttgga ggtcgggttg acatttcagg gaatgttcct
     4681 gaacgcaccc agcgactgac tgtcacacgc gacaggcctg taatacggga tatttccctg
     4741 aaactacatc ccttgttatg ctgggccata acctctgtcc aacgctgatg ccgttttttg
     4801 cgggtttgtt gttttaggcg ttccggacga cggagcgatg ctgcaggctc agacagttct
     4861 ggctcaggtg gtttctttgg tgacaactcg ctggcaacaa gacgtatcag aggtatatgt
     4921 ctgtacatca ttcgttcaag cgcatcgcca atatttttta gcaggtggca tcgaccacct
     4981 gtctggcctg tggtacacct tcacgtgctg ctgtcgcaca gaccccactg cgatcacgcg
     5041 agacaatctg tatttccgga tattttctga accaggtcgc cagcgtacgc tgatcacggc
     5101 cgggaagcag gacgagggga cggtgagtat caagattgac gattaacgta ccgtaacggt
     5161 ggccccgatg ccacgcccac tcatcaatac cgacatgagg cgcgcctgac tgtttcgtct
     5221 ccggggtatt gataaccctg cgaaggagag tatcagcact gcaacggaga cccactgccg
     5281 ttgctgcccg ttttccggcc tcacctccgg cgattaatcc cagttgacgc tgtaaatttt
     5341 gtaacgcctg tgaagactgc agatgtgaac caccgaaggg aacaaacgac tcggcaaaaa
     5401 tttttcgtga acaagccggg ttacggctgt acctgtggcg gacggaaaat acaagccata
     5461 gcgtctgccc tgagcagggt agatgctgta tccggcgccg acgacaacta tgaacagagt
     5521 cgctacgctt aaagcattca ggacattgtg ctgttttgcg ttttccgcgg agatggaggg
     5581 gtataccgtc agagttaata atttgtcgac tacatcgcca tccgcaaggt aactgtagaa
     5641 gtgttttaag agagtgcatt gcgttaccca taactaaaaa cagcttagat ccttgttcac
     5701 cataaagttt catccatttt ctccacccag gttgcggaag aaccactttt gcaccgttcg
     5761 tgacaggagc aggattttta cgccagtagc cagagccaga agtgggcgcg agacatcacg
     5821 tacttacgta cggatgaagg ctggctgtat ctgggagtgg ttaatgtcgc cacgtatgtc
     5881 tgcataagag acctcggaac gttatgctac cgcatgtcat ttgcaaaaat gacagataga
     5941 tatacttcaa tttaaacctt aacaattagc atgggaatgc tgtcaatcct ctgtatgcaa
     6001 aaatggcatc tgaatcgctc attatttagc cctctgtgtc aaagcgtaaa gtgttcccag
     6061 agagcctgta catagattcg tgtaatttcc tgattttgat atgttcatcg acaggaattt
     6121 atcccccgga aagattaaga aaacaaaaaa cgaacattta ttcaacacat ccggaacctc
     6181 atcgggtata tatcagcgct tatattgctg acattgattg atacagggta tcggtgacgt
     6241 ttttttgctc catttccctc aaaatggacg caacttcccc tctgcggctc tcagccgcaa
     6301 tactgcattc gggccagcgg ctcatgcatc ataaccagct ctgccagacg gtaaccccgc
     6361 ttcagccccg taaaccgcat ctgactttcg cacaggatgc atttcagcgg gtcaaccttc
     6421 agtaaccgct gatacatccc tctccacctg atttgcatcg ccgtttttat caccgtctct
     6481 gttattacgt acactaattc ttccagcaac cggcgtttcg ccggactaag gaacccgtag
     6541 aacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc cataaacgca
     6601 tcaccactca tcaataggta ttcttcacgt cgtgtgcggt gactgttgta acgcagtccg
     6661 atttcatcct gaccggcata gtgctccaga cggctcattg gcaccggcgg ctttttcagg
     6721 taagagccaa agtacaccgc cacatgtgtg gcgttatcca tcacctttga tacgttgaca
     6781 ttcctgcccc gtcggtaatg actgtccagg aaacgattcc attcccgttt actgcttcct
     6841 tcagcagcca gctcatccgg tatcaatagt tccgggtatt tccgtgacag taaccgtgtt
     6901 atccggtatc gctacatact catcacctta cgggcgtaaa aatgaaggtt tttccaggtg
     6961 tgatgtgatg tcacaccgcc ggcagttgtc gataaatgga taaatggata aatggatatg
     7021 tggatatgtg gatgacactg ctggtcccgg ccccatgtgt ggattaccgt gaatatcccc
     7081 ggcttcacat ctgctgctat gcggctcatc tctgccagta accaccggtt gtggaacacc
     7141 agggaccagt actggcaggg aagcgtgaat acaattgctg ccacgggccg tcggggacca
     7201 gactcagcag atactgtatc cactgtgcac ccgctttcac cccgcagtgt gggcaggagc
     7261 gttttttaca ccgaaaacag accttctttg tattgcaaca gtccgggcag tacatgccgc
     7321 cggcgctgat accggcaaga atggtcgtaa actcccgctc tgtacagcgg gcgatttccg
     7381 gatgcccttc gtcatcaaca cgtacaaacc agaagaccag ctttttgttt ccggcatcca
     7441 caaagaacgg aatattcagg tctgcgcaac actcaacagt atcgtcaaaa ttatcgaagc
     7501 gcagaatctc tacttcttct tcgtcaaaaa aatcatcttc gtgaagtttt acaacatagc
     7561 ggggaagttt gcttctttga ggtgcgagtt ttcgtttacg gggtttagct gaacgtgcca
     7621 tataactacc tgaaagacaa tgacattgtt tgtttttata acggtaattt caggccatga
     7681 caagccgcag ccgctaggct gcctactcga tcgataggaa tttaaatccc aaaaagatta
     7741 aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga gtgcaccgcc
     7801 gtcaattaaa tggatcttat cgattgatac tgcatatcac tgatggtttt ttaccccatt
     7861 cccctgaaaa tggacgcaac ttctccgatg cggctctcag cc
//
LOCUS       DAZUGK010000202         7662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZUGK010000202 DAZUGK010000000
VERSION     DAZUGK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.203"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1330
                     /gene="chbC"
                     /locus_tag="ACHY5W_003933"
                     /pseudo
     CDS             1..1330
                     /gene="chbC"
                     /locus_tag="ACHY5W_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            1381..1731
                     /gene="chbA"
                     /locus_tag="ACHY5W_003934"
     CDS             1381..1731
                     /gene="chbA"
                     /locus_tag="ACHY5W_003934"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFS4448721.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1739..2581
                     /gene="chbR"
                     /locus_tag="ACHY5W_003935"
     CDS             1739..2581
                     /gene="chbR"
                     /locus_tag="ACHY5W_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFS4448722.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2686..4038
                     /gene="celF"
                     /locus_tag="ACHY5W_003936"
                     /gene_synonym="chbF"
     CDS             2686..4038
                     /gene="celF"
                     /locus_tag="ACHY5W_003936"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4448723.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4051..4809
                     /gene="chbG"
                     /locus_tag="ACHY5W_003937"
     CDS             4051..4809
                     /gene="chbG"
                     /locus_tag="ACHY5W_003937"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFS4448724.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(4856..7117)
                     /gene="katE"
                     /locus_tag="ACHY5W_003938"
     CDS             complement(4856..7117)
                     /gene="katE"
                     /locus_tag="ACHY5W_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFS4448725.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDPHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNSLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7300..7563
                     /gene="cedA"
                     /locus_tag="ACHY5W_003939"
     CDS             7300..7563
                     /gene="cedA"
                     /locus_tag="ACHY5W_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFS4448726.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1886 a         1720 c         2010 g         2046 t
ORIGIN      
        1 aggtactcct cccttttgca gttaaaatag gaaagcagcc acacgttaat gcaatcaaaa
       61 atggctttat tcgcttaatg ccgttaaccc ttgcgggggc catgtttgta ttaattaaca
      121 acgtttttct aagctttggg gaggggtcgt tttttattcc ttaggtattc gcctggatgc
      181 ctcaaccatt gaaacactta atggtctgaa aggtattggc ggcaatgtat ataacggaac
      241 attagggata atgtctttaa tggcaccgtt ctttattggc atggcgctgg cagaagagcg
      301 taaagtcgat gcgctggcgg ctgggttgtt atccgttgca gcatttatga ccgtcacccc
      361 atatagtgtc ggtgaggcct atgcggttgg cgcaaactgg ttaggtgggg cgaatatcat
      421 ctccgggatt attattggcc tggtggtggc agaaatgttt acctttattg tccgccgcaa
      481 ttgggtcatt aaactgcccg acagcgtacc tgcttcagta tcgcgttcct tctcggcatt
      541 aattcccggc tttattattc tttccgtgat ggggattatt gcctgggcgt tgaatacctg
      601 gggcaccaac ttccatcaga tcattatgga taccatctca accccactgg catcgttggg
      661 tagcgtggtg ggctgggcct atgtgatctt tgttccactg cactggttct tcggtattca
      721 tggcgcgctg gcgctgaccg cactggacaa cggcattatg acgccgtggg cgctggaaaa
      781 tatcgcgacc tatcagcaat atggttccgt cgaagcggcg ctggcagccg gtaagacctt
      841 ccatatctgg gccaagccga tgctggactc ctttattttc cttgggggca gtggtgcgac
      901 tttaggcctg atcctggcta tttttatcgc ctctcgccgt gctgattatc gtcaggtggc
      961 aaaactggcg ctgccgtccg gcatcttcca gattaacgaa ccgattctgt ttggtctgcc
     1021 aattatcatg aacccggtga tgtttatccc gtttgtactg gtacaaccga ttctggcggc
     1081 aatcaccctc gcagcgtact acatgggcat tattcctccg gtgaccaata tagcaccgtg
     1141 gaccatgcca accggtctgg gagccttctt taacaccaac ggtagcgtcg ccgcattgct
     1201 ggtcgcactc ttcaaccttg gcatcgcaac gttaatttat ctgccctttg ttgtggtggc
     1261 taacaaagca caaaatgcga ttgataaaga agagagcgaa gaagatatcg ctaacgccct
     1321 gaaattttaa tcgatgccgg tacgggtaat cgtgccggta agaatagaga ggaacgatgt
     1381 atgatggatc tcgataatat tcccgatacg caaacggaag ctgaagagct ggaagaagtg
     1441 gtgatggggc ttatcatcaa ctccggacaa gcgcgcagcc tggcgtatgc ggcactgaaa
     1501 caggcgaagc agggcgattt tgccgcagca aaagccatga tggatcagtc acggatggca
     1561 ttgaatgaag cgcatctggt acagacgaaa ctgattgaag gcgatgcggg cgaaggtaag
     1621 atgaaagtga gtctggtgct ggtccacgct caggatcatt taatgacgtc catgcttgcg
     1681 cgtgaactga ttaccgaatt aattgagctt catgaaaaac tgaaggcata aggagtcgat
     1741 gatgcagcca gtgattaacg cgccggaaat tgccactgcc cgagaacagc agttgtttaa
     1801 tggcaaaaac ttccatgtgt ttatctataa caaaactgag agtatcagcg gactgcatca
     1861 gcacgactat tatgaattta ctctggtatt aaccgggcgt tatttccagg agattaacgg
     1921 taagcgcgtg ttactggaac ggggcgattt tgtttttatt ccgttaggtt cgcaccatca
     1981 aagtttttat gagtttggtg ccacgcgtat attgaacgtt gggatcagta aacgcttttt
     2041 tgagcagcat tacctgccgt tgttgcctta ttgctttgta gcttcgcagg tataccggac
     2101 caataacgct tttctcacct atgtggaaac agtgatttct tcattgaatt tccgcgaaac
     2161 agggctggaa gagtttgttg agatggttac tttttatgtc attaaccgtt tacgtcatta
     2221 ccgcgaagaa caggtgattg atgatgtacc gcagtggctg aaaagtacgg tagaaaagat
     2281 gcatgataaa gagcagttta gtgaatcggc gctggagaat atggtgacgt tgtcagccaa
     2341 atcacaggaa tatttgacgc gggcgactca acgatattat ggcaaaacgc caatgcagat
     2401 tattaatgaa atccgtatta attttgccaa aaaacaactg gaaatgacca actattcagt
     2461 gacggatatt gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa
     2521 gaaattaacg tcctttacgc ctaagagcta tcgtaagaaa ttgactgaat ttaatcagta
     2581 aatcgttata cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg
     2641 ggcagatatg ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta
     2701 aaagtcgtca ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt
     2761 aagcgttatc acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga aggtggcaaa
     2821 gctaaactgg atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg
     2881 atgaagcttt ataaaacgct ggatcgccgc gaagcattga aagatgctga tttcgttact
     2941 acccaactgc gcgtaggcca attaccggcg cgcgaactgg atgaacgtat tccattaagt
     3001 catggttatc ttggtcagga aaccaacggc gcgggcggtc tgtttaaagg tctgcgtacc
     3061 attccggtga tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg
     3121 attaacttca ctaacccggc gggaatggtc actgaagccg tttatcgtca taccggattt
     3181 aaacgcttta tcggcgtgtg taatattccg atcggcatga agatgtttat tcgcgatgtt
     3241 ctgatgctga aagacagcga tgatttatct atcgatctgt tcggcctcaa ccatatggtg
     3301 ttcattaagg atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg
     3361 gcatccgggc agttaaaagc atctggcgtt aaaaatattt tcgatctgcc atttagcgaa
     3421 ggcttaattc gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa
     3481 aaagagatgc tggctattga aatgggtgaa tactacaaag gcggcgcacg agcgcaagta
     3541 gtacagaaag tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag
     3601 ccgaaagaac tggaacagcg cggtggggct tattactctg atgcagcatg cgaagtgatc
     3661 aacgctatct acaacgacaa gcaagcagaa cattacgtta atatcccgca tcatgggcat
     3721 attgataata ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc
     3781 gcgacgccac atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc
     3841 attaaaggct tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg
     3901 ttactggcgc taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca
     3961 cgcgagatga ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag
     4021 cttaaaaaag cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat
     4081 gattttggct taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt
     4141 gtcacatcga cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt
     4201 cgtgatgaac cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg
     4261 acagctatgc cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca
     4321 gaagaagatg ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt
     4381 ttcattgagc tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg
     4441 ttcccgcaga ttttccctat tgttgccagg tttgcggctg aagaggggat tgcgttacgc
     4501 atcgaccgtc agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcac
     4561 ggattcagta gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctt
     4621 gatgattcca gccatcgcgg ggaacgctcg ctggaagtca tgtgccatcc ggcgtttgtc
     4681 gataatacga ttcgccagag tgcttactgt ttcccacggt taacggagct ggaagtgctg
     4741 acatcagcgt cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt
     4801 gatgtgtaag taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg
     4861 ccggaatttt gtcaatctta ggaatgcgtg accacacgcg gtgtgctgcc atcagcgtta
     4921 acagttcatc cataaaacta ccgtcggcgc tgtcagcttc cacaatccct tcttcaccct
     4981 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt
     5041 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
     5101 cgatattgcc gcaagggaca atgaccgcat cgaccgtcag cgaaggcgca ccggcaaagg
     5161 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
     5221 gcagtttggc atgaacgcct ttggccttca gggccttgag aatggccaga aggtctgccg
     5281 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
     5341 tggcgtacaa acttaaggat ggatcctttt tcagactgtt gacgtccgga ggtggggtga
     5401 tattcagctg gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag
     5461 tgagatcaat atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
     5521 tgcttaactc aaaactgaaa ccatcgacaa tatgacgctg ctcgaatggc gtctgactta
     5581 gccagaacag acgcggatgg gaataatatt cgccaaaaga tggactgcgc tcgcgaactt
     5641 tatttccttc cacgcgttcc tgatatgatt caaaaccgcc gcgtttcggt cccggcggtg
     5701 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta attcgccggg ttagtgtcga
     5761 tccccatacg atgcatgcca tcacgctgga agttgtggta agggcaggtc ggacggttaa
     5821 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     5881 acaaacgtcc ctgcaacagc ggatcgttgg tgaagtccag tcccggcacg atatgcccag
     5941 gatggaaagc cgcctgttcg ttttcagcaa agaagttatc cgggttgcga ttgagcacca
     6001 ttttgccgac acgctgaacg ggcaccagtt cttccgggat aagtttggtt ggatcgagaa
     6061 gatcgaagtc gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt
     6121 ccggaaaatc gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac
     6181 gtccggttag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     6241 agtggaaacg tacaaacgtt gccttccctt cggcattaat caggcggaag gtatgaatac
     6301 caaagccttc catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca
     6361 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcgcttt
     6421 gcccttgtgg aattgcccag tgtggttctg gttttaccgc atgcacaaaa tcggggaatt
     6481 tatgcggatc ctggataaag aagattggcg tgttattgcc aacaaggtca aaaataccct
     6541 cttcggtata gaatttggtg gcaaagccac ggatatcacg cacggtatcg gcagagccag
     6601 caccaccctg aacagtagag aacctaacaa atactggggt gattttgttc ggatctgaga
     6661 ggaaatccgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     6721 ctgatccacg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt
     6781 tctcgcgcag aataaaatct tccagcagcg ttggaccacg gctaccggca cgcagtgagt
     6841 tttgatcgtc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     6901 tgcgtacgtc ttccagagaa ttaagttttt cgttacgcgt atcaggggct ttcaggctcc
     6961 ctggggcggt aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagagccgt
     7021 cctcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     7081 ggtgctgatg tgggttcttt tcgttttgtt gcgacattga actagtctcc ttaatttatt
     7141 actgaaaggg ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagaccc
     7201 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg
     7261 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     7321 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     7381 cccgcgccgc cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt
     7441 ggcaaatatg ttgcgtttgt actgatggga gagtcatttt tgcgctcacc ggcgtttact
     7501 gtgcctgaat ccgcccagcg ttgggcaaat cagatccgcc aggaagggga agtgactgag
     7561 taaggattac gtacctcccg gttttgccgg gagttacatg gcctgaaatt ttgtatttca
     7621 ttgctacatt cattcaaacc acctgtaaca aaaagctgcc tt
//
LOCUS       DAZUGK010000203         7594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZUGK010000203 DAZUGK010000000
VERSION     DAZUGK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.204"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            286..4539
                     /gene="fdeC"
                     /locus_tag="ACHY5W_003940"
     CDS             286..4539
                     /gene="fdeC"
                     /locus_tag="ACHY5W_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFS4448727.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLQKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLIAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQNNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTATGEETMK"
     gene            complement(4660..5517)
                     /locus_tag="ACHY5W_003941"
     CDS             complement(4660..5517)
                     /locus_tag="ACHY5W_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448728.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIYPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5775..6644
                     /locus_tag="ACHY5W_003942"
     CDS             5775..6644
                     /locus_tag="ACHY5W_003942"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4448729.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     DQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6804..7397)
                     /gene="rclC"
                     /locus_tag="ACHY5W_003943"
     CDS             complement(6804..7397)
                     /gene="rclC"
                     /locus_tag="ACHY5W_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFS4448730.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7409..>7594)
                     /gene="rclB"
                     /locus_tag="ACHY5W_003944"
     CDS             complement(7409..>7594)
                     /gene="rclB"
                     /locus_tag="ACHY5W_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFS4448731.1"
                     /translation="FSTNADDKIILKHISVSSVSASPTVLEDAIADIARKYNASSWKV
                     TSMRIDNNSTATAVLYK"
BASE COUNT         2082 a         1682 c         1944 g         1886 t
ORIGIN      
        1 attattcact gtccgcagag taaaaaatat aacttaggta tctatttaat gacttgcaca
       61 aaaagctaaa ttttcaccca taaataaaaa tataatcccg cgcccaacca cctgatgagt
      121 ggctacaggc actggatata ttaggtggcg gtgtactttc ttacataaag gtatttcctt
      181 ttctgcggaa aaggaaaccg ggaaatcccc ggtttttctg acaagcagac gccattattt
      241 gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc acgttataaa
      301 acaggtcata aacaaccacg atttcgttat tcagttctgg cccgctgcgt ggcgtgggca
      361 aatatctctg ttcaggttct ttttccactc gcagtcactt ttaccccagt aatggcggca
      421 cgtgcgcagc atgcggttca gccacggttg agcatgggaa atactacggt aactgctgat
      481 aataacgtgg agaaaaatgt cgcgtcgttt gccgcaaatg ccgggacatt tttaagcagt
      541 cagccagata gcgatgcgac acgtaatttt attaccggaa tggccaccgc taaagctaac
      601 caggaaatac aggagtggct cgggaaatat ggtacagcgc gcgtcaaact gaatgtcgat
      661 aaagatttct cgctgaagga ttcttcgctg gaaatgcttt atccgattta tgatacgccg
      721 acaaatatgt tattcactca gggggcaata catcgtacag acgatcgtac tcagtcaaat
      781 attggttttg gctggcgtca tttttcagga aatgactgga tggcgggggt gaacaccttt
      841 atcgaccatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga atactggcgt
      901 gattatttga aactgagcgc caatggttat atccgggctt ctggctggaa aaaatcgccg
      961 gatgttgagg attatcagga acgcccggcg aatggctggg atattcgtgc tgagggctat
     1021 ttacccgcct ggccgcagct tggcgcaagc ctgatgtatg agcagtatta tggcgatgaa
     1081 gtcgggctgt ttggcaaaga taaacgccag aaagatccac atgcgattac cgctgaagtg
     1141 aattacacgc cagtacctct tctgaccctg agtgccgggc ataagcaggg caagagtggt
     1201 gagaatgaca ctcgctttgg cctggaagtt aattatcgga ttggcgaacc tctgcaaaaa
     1261 caactcgata cagacagcat tcgcgagcgt cgggtactgg caggcagccg ctatgacctg
     1321 gttgagcgta ataacaacat cgttcttgag taccgcaaat ctgaagtgat ccgtattgct
     1381 ctgcctgagc gtattgaagg taagggcggt cagacacttt ccctggggct tgtggtcagc
     1441 aaagcaactc acggactgaa aaatgtgcag tgggaagcgc cgtcattact ggctgaaggt
     1501 ggcaaaatta ccggtcaggg tagtcagtgg caagtaacgc tcccggctta tcgtccaggc
     1561 aaagacaatt attatgcgat ttctgcggtt gcctacgata acaaaggcaa tacctcaaaa
     1621 cgcgtgcaga cagaggtggt cattaccgga gctggtatga gcgccgatcg cacggcgtta
     1681 acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca aaaaccgctg
     1741 gtgctgtctc tgcgcgacgc cgagggccag ccagtcacgg gcatgaaaga tcagatcaag
     1801 actgaactag ccttcaaacc ggctggaaat attgtgactc gttccctgaa ggccactaaa
     1861 tcacaggcaa agccaacact gggtgagttc accgaaactg aagcaggggt gtatcagtct
     1921 gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt tgatggcatg
     1981 agcaaaaccg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa ttccaccctg
     2041 agcgctaacg agccgtcagg tgacgtggtt gctgatggtc agcaagccta tacgttgacg
     2101 ttgattgcgg tggactccga gggtaatccg gtgacgggag aagccagccg cttgcgattt
     2161 gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa accaggcgtt
     2221 tacagcgcca cggtttcttc aacccgtgcc ggaaacgttg ttgtgcgtgc tttcagcgag
     2281 cagtatcagc tgggcacatt acaacaaacg ctgaagtttg ttgccgggcc gcttgatgca
     2341 gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttgggggtac agttacggca
     2401 atctggacgg taaaagatgc ctatgacaac cctgtgacca gcctcacgcc ggaagcgccg
     2461 tcattagcgg gtgccgctgc tgaaggttct acggcatcgg gctggacaaa taatggtgat
     2521 gggacgtgga ctgcgcagat tactctcggc tctacggcgg gtgaattaga agttatgccg
     2581 aagctaaatg gacagaatgc ggcagcaaat gcggcaaaag taaccgtggt ggctgatgcg
     2641 ttatcttcaa accagtcgaa agtctctgtc gcagaagatc acgtaaaagc cggcgaaagc
     2701 acaaccgtga cgctggtggc gaaagatgcg catggcaacg ctatcagtgg tctttcgttg
     2761 tcggcaagtt tgacggggac cgcctctgaa ggggcgaccg tttccagttg gaccgaaaaa
     2821 ggtgacggtt cctatgttgc tacgttgact acaggcggaa agacgggcga gcttcgtgtc
     2881 atgccgctct tcaacggcca gccagccgcc accgaagccg cgcagttgac ggtcatcgcc
     2941 ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaggctcc gaccgtcaaa
     3001 acgacgacgg aactcacctt caccgtgaag gatgcgtacg ggaacccggt caccgggctg
     3061 aagccagatg caccagtgtt tagcggtgcc gccagcacgg ggagtgagcg tccttcagca
     3121 ggaaactgga cagagaaagg taatggggtc tacgtggcga ccttaacgct gggatctgcc
     3181 gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc tcagccactg
     3241 gtgctgaatg ttgctggtga cgcatctaag gctgagattc gtgatatgac agtgaaggtt
     3301 aataaccaac tggctaatgg acagtctgct aaccagataa ccctgaccgt cgtggacagc
     3361 tatggtaacc cgttgcaggg gcaagaagtt acgctgactt taccgcaggg tgtgaccagc
     3421 aagacgggga atacagtaac aaccaatgcg gcagggaaag tggacattga gcttatgtca
     3481 acggttgcgg gagaacacaa tatttccgct tcggtgaatg gtgctcagaa gacggtcacg
     3541 gtgaaattca acgcggatgc cagcaccggt caggcaaacc tgcaggtaga caccgccgtt
     3601 caaaaagtgg caaacggcaa agatgccttt acgctgacgg cgaccgttga ggataaaaat
     3661 ggtaaccctg ttccagggag cctggtgacc tttaatctgc cccggggtgt caagccgctt
     3721 acaggcgata atgtctgggt gaaagccaac gatgagggga aagcagagtt gcaggtggtt
     3781 tcagtgactg ccggaacgta tgagatcacg gcatcggcag ggaatagcca gccttcgaat
     3841 acgcagacta taacgtttgt agccgataag gctaccgcaa ccgtctccgg tattgaggtg
     3901 attggcaact atgcgctggc ggacggcaaa gccaaacaaa cgtataaagt tacggtgact
     3961 gatgccaata acaacctgtt gaaagatagc gaagtgacgc tgactgccag cccggcttcg
     4021 ttaaacctgg aaccgaatgg cactgcgaca acgaatgagc aagggcaggc tattttcacc
     4081 gctaccacta ctgttgcggc gacatacaca ctcaaggcgc aagtgagtca gaacaatggt
     4141 caggtatcaa cgaaaactgc cgaatctaaa ttcgttgcgg atgataaaaa cgcggtactc
     4201 accgcatcat ctgatatgca atctctggtg gcggatggga aatcgactgc gaagctggag
     4261 gtgacactga tgtcggcaaa caaccccgtt ggggggaata tgtgggtcga cattcagacg
     4321 ccggaagggg tgacggagaa ggattatcag ttcctgccgt cgaaaaatga ccatttcgtg
     4381 agcggaaaaa tcacgcgtaa atttagtacc agcaagcctg gtgtctatac gttcacattt
     4441 aacgccctga cgtatggcgg gtacgaaatg aagccagtga cggtgaccat taccgcggtg
     4501 gatgccgata cggcaacggg cgaggagacg atgaaataat attaagaata tgaaagtaac
     4561 attctaataa acaataatgg cgttgacatc ttcaacgcca ttatttattg aattgcctga
     4621 cagtagtctc gttataagtt catttagata tagcgacagc taaaccggac gaacggggat
     4681 aaaaatttcg caaacaatgt tattgctatc aatttcgacg aagttttcat tgtaataaaa
     4741 ccgctcaata tcactgccgt tcactctggc taaccccctg gcgggtaatt ccatcagata
     4801 gaggtttcgc gcccattcag ggtagtgctc ccactcacct tcatagcgga aagaggcata
     4861 cagtcctcgg ttaattgtcc actctttcgg catctctgaa tcggtttgtt tgcataccgt
     4921 accggcaaaa gtttcaaccg tgagcaaatc gaccgatttt gttgacggtt caaacgtgga
     4981 gacgaaatag atatccgtct taaaattatt catacatctg gctaaatgat gacgtcgcag
     5041 ttttacttct tcatccggtg atttattcga cacttcaata tcatatttaa atgaattgcc
     5101 aaaaataggt gtctcaggaa agttgactaa tctacactcc gtcttttgct gttgacgtat
     5161 taaaaaagaa gggtagatat ttgcgagatc ccaataatca cggtggcggt atacacgggg
     5221 agagcagccg aataactttt taaattcgcg gctgaatgac tgctgtgaat caaaatgcaa
     5281 agagagtgca atatcaagca tagatttcgc ggtgagccgg acaagaatgg cggcacgaca
     5341 aagccttcgt ttgcgaatgt attctcccaa aggcacatgc atgaaatttc ggaacagaag
     5401 ctggatgttg cgtctgctgt agccagattt ctgtgcgata tcttcgattg aaatagggtg
     5461 ctcaagattg cactcaatcc actccaggag ctgctgtaga atcttctgcc tgatcatcac
     5521 attctccgtt aattctttgt catatcgcac attttcgtta tgccctgggc cctaagtgta
     5581 tcaaaaacag caagggcgaa ggcaaaagca ctggtagcgt ctgcttcgtt gtccccgaaa
     5641 atagagaggt ttcagtccta cattattaat gaattttttg cataagtgat atcaaaatcc
     5701 acatactaat ttgaggttac gttttaacgt agactcattg ttcatgccta atgtgcctaa
     5761 tggagggact gacagtggaa ttttccgtat taagtaacaa tctgaaaatg ccgatgatgg
     5821 gatttggtgt ttttcaggtt accgataaaa acgtgtgtaa acagtcagtg ttgaacgcta
     5881 tccgcacggg ttatcgactc atcgacactg cagcggtata cggtaatgaa gatgctgtcg
     5941 gtgaagccgt tcgtgaagcc atatccgaag gtttatgtac ccgagaagag ttatttataa
     6001 cgtctaaatt gtgggtgcag gatatgttga atcaggatac ggcagcagca ggtattgaag
     6061 catcattaaa aaaatcggga ctagaatatt tcgacctcta tttattgcat caggctatgc
     6121 gcgattattt cagtgcgtgg cgtgcactcg aagatgccta tgaagagggc aaattaaaag
     6181 cgattggggt ttccaatttc tatcctcatg ttctggcgaa cttttgtgaa acggtaagag
     6241 ttaaaccgat ggtcgaccag gtcgagttgc atccctattt tgcccaacca gaggcgctgg
     6301 caaccatgaa gtattataac gtgcagcctg aagcatgggc tccgttaggt ggtggacgac
     6361 ataaaccctt tgaaaataat ctgcttcaga gtattgcaga tgcccatcaa aaatcgattt
     6421 ctcaagtcat tctgcgttgg aatattcaac ggggagtggt cgttattccg aaatcgacac
     6481 atcaacagcg tatcgaagaa aattttgcta tctgggattt ctcactgaca gagaaagaaa
     6541 tggcacaaat tagttcgctt gatttgggtt atgttgggga gtcggtaaaa cattttaatc
     6601 ctgaatttgt tcgtggttgt cttgctgtaa aaatacatga ttgatattaa tcatgtattt
     6661 tacctgagac gacaagaatc ttttaacagg ggagtgatat tgatcttcac tctgtcatat
     6721 ctccggtaat atggcgtcag gcttcatcat cagaaagggg gcctgacgcc tgaaaaagtg
     6781 aacaacagac agtgttcgga ttatcaatat tcagttttta acgttgaact ggattcatta
     6841 ctgcgttgtt taagaatttc ccgcgccgaa tctgccatta tcatgacagc acctgccagc
     6901 atcagagtat ctttcaatac caggcgacca gcaccggata aataagggaa accatgatga
     6961 gcgtcaccca atgcgggtac ccatgcctcc ggggtggtga ttaaaaatga gagtgttacc
     7021 aacggtgtgg taaatgccat cagccctccc aataaaccta accagcgatt gacaggatta
     7081 gccaaaacca gcagagcaat aatcacctcc acgacgccaa gaccgttgga aaaaccatag
     7141 gtattattgg ccgattgcca tgcccttgct tctggtttgt attcgccttc gtgagtcaga
     7201 tactgtttat agtcttccgg gtgttcataa aagaacgaca ttagtggact gtttgcgacg
     7261 aatggtgtaa tgctgtctgc ctcgtaaggg acaaacttta ataacccaat ccacataaaa
     7321 acaatcgcaa tactcagacg aatcaatgtc aggccaattt tatctccccg acttaacagg
     7381 tgcaggtatt tttccattgt gaacatcctt atttatacaa tactgctgtt gcggttgaat
     7441 tattatcaat tcgcatcgat gtgactttcc aagatgaagc attatatttt ctggctatat
     7501 cagcaatggc atcctccaga actgtcggtg atgctgatac tgacgagacg ctgatatgtt
     7561 tcagaattat tttatcatct gcattggtgg aaaa
//
LOCUS       DAZUGK010000204         7575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZUGK010000204 DAZUGK010000000
VERSION     DAZUGK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.205"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..240)
                     /locus_tag="ACHY5W_003945"
     CDS             complement(<1..240)
                     /locus_tag="ACHY5W_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFS4448732.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKNCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISC"
     gene            498..995
                     /locus_tag="ACHY5W_003946"
     CDS             498..995
                     /locus_tag="ACHY5W_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HFS4448733.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTTGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLATVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            1087..2019
                     /locus_tag="ACHY5W_003947"
     CDS             1087..2019
                     /locus_tag="ACHY5W_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4448734.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAADELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSLDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAYVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2061..3149)
                     /locus_tag="ACHY5W_003948"
     CDS             complement(2061..3149)
                     /locus_tag="ACHY5W_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4448735.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(3291..7274)
                     /gene="ehaA"
                     /locus_tag="ACHY5W_003949"
     CDS             complement(3291..7274)
                     /gene="ehaA"
                     /locus_tag="ACHY5W_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFS4448736.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGVHSISTLGFSGG
                     TVDFGALAAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVMGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGLTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         1839 a         1957 c         1673 g         2106 t
ORIGIN      
        1 acagctaatt aaaaaaggat ttaaacaggt atgatcttgt ttattaatat ccgctccggc
       61 gtcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
      121 tttcccctga cggtcgcagg tattaatatc gacacccagc gcaaggcagt tttttacttt
      181 atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt ttatactcat
      241 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
      301 ataaaagaac ccgcttaaat ttaattcgcc gtatttcgcc tttttgttga atgatgttca
      361 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taccctttca
      421 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc gtccatgtct
      481 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
      541 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
      601 gaacgacagg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt aaatcggggt
      661 ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcgtttttc gccgcgctga
      721 ctgccggttg gctggccact gtaagtggac tgtccgcgtt ctgggcgagc gtaattacta
      781 ccgtaccctt ctcggcggtg gtggtctggc aagggcgttt ctggttactg tcatttattc
      841 ccggtggctt tctcggcatg acgctgtttt ttgcctgcgg gatgaactgg acggtgacgt
      901 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggc ggacaaaaat
      961 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
     1021 ctttaatcga tacctctaca gtcatggctg gagaaattca tattagataa tggcgggaat
     1081 ggtttaatga actcagtttt taccgaagag aatctattgg catttactac cgctgcgcga
     1141 tttggcagct tcagtaaagc cgcagacgag ttgggtttaa ccacttccgc cattagctac
     1201 accattaagc gtatggaaac ggggctggat gtggtgttgt tcactcgcag tacccgcagc
     1261 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgactt actgaatgat
     1321 tttcacgcca tcaaacgcag tctcgatact atttcgcagg gcatagaagc acgggtgcgc
     1381 atttgtatta atcagctgct ttatacgccc aaacacaccg cgcgcttatt acaggtgctg
     1441 aaaaagcagt ttcctacctg ccagattacc gtgacgaccg aagtgtataa cggcgtctgg
     1501 gatgcgatta ttaataatca ggccaatatc gctatcggcg cacccgacac gctgctggac
     1561 ggtggcggca ttgattacac ggagattggc gcgattcgct gggcttttgc cattgccccg
     1621 gatcacccat tagcctatgt gccggaaccc atcgccgaaa gccagctgcg tctctaccct
     1681 aatatcatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg gttgctgcat
     1741 gggcaggagt caattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
     1801 gaagggattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca atccctgctg
     1861 gttacccgac aaatccataa tccgcgccag gattcgcgca tgttgctggc gactcagcat
     1921 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa tggcatatta
     1981 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
     2041 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
     2101 ttacccggaa ctggcgggga gtacaaatag ccctgcaaaa agtgaacgcc tttaccgatc
     2161 attaaatccg cctgctcctg ggtctctacc ccttccgcta cgatactcag accaggctta
     2221 cgcgccagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac actcgccatt
     2281 tgcacaaatg acttatcgat cttaataaaa tcgaccggaa acgcctgcag ataacgatag
     2341 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
     2401 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
     2461 accagtttga ttttatcgtt tcctaatttc ttgaccaggt tcaggcattc tttttcaaat
     2521 cccgcggcca aaaaacaacc cgccgagacg ttgataccaa tatggaaatt atccggcagc
     2581 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg ggtcattatg
     2641 acaataagac cggatgactc cgccagagga ataaactgat ccggcgggat gattcccgtt
     2701 tgtggatgtt cccagcggac aagcacctca cagcccgtca gtaccccagt ctgcgcgcag
     2761 aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact gatggcttca
     2821 ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt atttcccgtg
     2881 ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt aatatcactc
     2941 tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt tgcactgcga
     3001 tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt gagtaaaagc
     3061 cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc gaactcttcc
     3121 aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc ttgtgcgcca
     3181 gctaaatctg cgagtcttca ctctgacgga taaagctggc tgcaagaaaa tgacacagag
     3241 agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga tcagaattgc
     3301 cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac ctgaacgccc
     3361 acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt ttttatctca
     3421 gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt gctgaaatcc
     3481 ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga tttaccgtca
     3541 tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg aacattgcca
     3601 tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc tttgactccc
     3661 atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc ctggctgcca
     3721 ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaag tccttttgat
     3781 ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca gctgtactgc
     3841 gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca ggtggcatag
     3901 aggcctgtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg cgaggaaatg
     3961 gttttgctgt cgctgttgcc atatcccgcc atgaccccaa catgccagct gtcgctgccg
     4021 ttttggctcc actgcgcgac atcacctccc agttgcagaa catagcgatt gctttgggtt
     4081 ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg catccacata
     4141 gtggtttgtt tctgctcacc catcaccatg tcggtatagt acgtattacc cagacgctca
     4201 tgcagacgcg tggtgaacat ggtattcgct gctgcaaggt tcgcaatgta gctacccgcc
     4261 tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt cgggttaggg
     4321 ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc cggctgcagt
     4381 tcaggagaat cactgccgct ggtcagatac cagttaccac tatttgcccc ttgtccacgc
     4441 gcgagagtgt agtcatacgc ccccgcaacg atacgcccag cctgaacaaa ttcgccctca
     4501 gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc tgtaccgcca
     4561 gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac caatttatcg
     4621 gttactgagt catcgccatt cagtaccgta ttcattacta gttgaccgtt attaccgtgg
     4681 tagttcccct tcaccactaa cacattgcca gcttgctgac ctgacattcc ggtcatcagc
     4741 gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc cgctcttttt
     4801 tcagcgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct acccaatccc
     4861 tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc cgtcaggttg
     4921 ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc gctccccgtt
     4981 aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt taagctcagc
     5041 agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt catatccagt
     5101 tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc gttattcaac
     5161 aaaagcgttc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt gttagaaagc
     5221 gatacggtct gacccttctg gctgctgaat gccaggtcac cactgccggt aatctttgcg
     5281 ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc atcgctgtcg
     5341 gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc attgtttacc
     5401 ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt ctgctgaata
     5461 tcacgttggg cgctgctgga gaggatttgc ccatttttat cctgcaattg cagattgccc
     5521 gcatcgccca gaacttccgc accttgcgca tcaaccaact taatggtgct gttaccatca
     5581 tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagct tacaacgtca
     5641 ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact aacgttgacg
     5701 gtcccttctg tcatctgtgc accagctgcc agggcaccaa aatcaaccgt tccgccggag
     5761 aatcccagag tgctaataga atgaacgccc ttacctactg ttacctgact tccctgaccc
     5821 gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa tgtcgtgttc
     5881 tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa ggagaattcg
     5941 tgattcccca tgtttatttc aacaatgcct ctaccttcaa gatcgttatc aaatacccaa
     6001 tctttgttgg cattaatttg caatttgcca tcaacatcca ggtccgcacg tccgatgttc
     6061 ttgtcttcag aaataaccgc gacagcacca gaagcgacat caatatatcc gctgaatgca
     6121 tagttattgc cggtcagtgc cactgtccca ctgtcgatac ttattgtacc attgccggaa
     6181 atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc gcggttgttg
     6241 atttctgctg tacctatatt ttgggtgatc cctaaagcgc cattttgctc aattttgact
     6301 ttaccgctaa aggcggagtt atccccggtt aagtaagtcg tgccattgag cgcattgata
     6361 tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtt tttatccgtg
     6421 tggtttaagg tgattttctt ctcggatgtc tttgcccaca caaactcaat tttttcggca
     6481 tcaataatcc ctgcagcctt cgctgcgctt ccttcctgtg cacctaacgc taactcagag
     6541 ttggtgctta aactaatagt cggcgcagag attttaccac cttccgaaac agcaagtgtt
     6601 gtatcggagt tttgatcata ataacccagg ctaattttct cactggcatt aacttcaccg
     6661 ccgttattaa ctgacaggta ctgattgcca ccgtaactgc cgtaaacgcc cataaagaag
     6721 gttttagaat ttacctggct gccttcaccg ctgacatttc cctgcaaata ggtcagacgg
     6781 ctggtgccct taacatggat tttgccgccg ttggaaacat taaaatagcc gcgatcgtgt
     6841 ctcatgcctg agtttgattc atacaaatca tcagcatata actcgctgcc agcattggag
     6901 acattcagga accccgtacc tttcagcaaa aagttaagtg ggccattgat tgttaccttt
     6961 cctccggtaa tatccaaccc accaccagaa gcgagagtca gacgcggacg ggaagtatct
     7021 gtcgcattga ttaccagatt ggcattgtct gtcaccgtaa tacggtaatc gatgctctgg
     7081 ttctctttgc caaaatcaat agttttatcc tggtttacga tgacatcatc ggcgaatacc
     7141 agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt tccttttact
     7201 ctgctcccca gctccgagca gacgctccaa cagttacggg acctgttcca ctttagccga
     7261 tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga ttgcataaac
     7321 agcaacgcat taaatgccat tccggttact gttcatcgca tataaaaatt caaaaatttt
     7381 gcatagcatt ggcaaccaca tatctgagta tgtagttcag agggtaataa aatagcaagt
     7441 tactactgaa aacttatttt aagttaacac tcttaacttt atcattacat tccccccagt
     7501 ctctaatacc tgcgcattat atgtatccgg gaactcaaat caactcaaaa tataaacaaa
     7561 agcaggagaa ataca
//
LOCUS       DAZUGK010000205         7546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZUGK010000205 DAZUGK010000000
VERSION     DAZUGK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.206"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..886
                     /locus_tag="ACHY5W_003950"
     CDS             <1..886
                     /locus_tag="ACHY5W_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4448737.1"
                     /translation="ELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGA
                     RLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPF
                     IRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVI
                     VHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPV
                     ITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHH
                     EQSPAARNFRIHLLNALR"
     gene            1021..2241
                     /gene="mlc"
                     /locus_tag="ACHY5W_003951"
                     /gene_synonym="dgsA"
     CDS             1021..2241
                     /gene="mlc"
                     /locus_tag="ACHY5W_003951"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFS4448738.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2366..3061
                     /gene="bioD"
                     /locus_tag="ACHY5W_003952"
     CDS             2366..3061
                     /gene="bioD"
                     /locus_tag="ACHY5W_003952"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS4448739.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3014..4306)
                     /gene="clcB"
                     /locus_tag="ACHY5W_003953"
     CDS             complement(3014..4306)
                     /gene="clcB"
                     /locus_tag="ACHY5W_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFS4448740.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TEGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4465..5079)
                     /gene="dmsD"
                     /locus_tag="ACHY5W_003954"
     CDS             complement(4465..5079)
                     /gene="dmsD"
                     /locus_tag="ACHY5W_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFS4448741.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYTPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5122..5976)
                     /gene="ynfH"
                     /locus_tag="ACHY5W_003955"
     CDS             complement(5122..5976)
                     /gene="ynfH"
                     /locus_tag="ACHY5W_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFS4448742.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTIPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5978..6595)
                     /gene="ynfG"
                     /locus_tag="ACHY5W_003956"
     CDS             complement(5978..6595)
                     /gene="ynfG"
                     /locus_tag="ACHY5W_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFS4448743.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYLRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6606..>7546)
                     /locus_tag="ACHY5W_003957"
     CDS             complement(6606..>7546)
                     /locus_tag="ACHY5W_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFS4448744.1"
                     /translation="EMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYV
                     ILAQPATSAKFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPE
                     MPDYEEMKTTGIFKKKCPEEHYVAFRAFSEDPQANPLKTPSGKIEIYSERLAKIADTW
                     ELKKDEIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQ
                     EVWINPIDAQARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFG
                     DRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         1920 a         1942 c         1968 g         1716 t
ORIGIN      
        1 tgaacttcgt catctgcgct actttgttgc ggttgcggaa gaactgcatt tcgggcgcgc
       61 cgctgcccgc ctgaatattt cgcaaccgcc gctaagtcag cagattcagg cgctggagca
      121 acaaattggt gcccgactgc tggcacgaac caatcgcagt gtattgctga cggcagcagg
      181 aaaacagttt cttgcagata gtcggcaaat cctgtctatg gtggatgacg ctgccgctcg
      241 cgctgaaaga ctgcatcagg gtgaagcggg ggagttgcgc attggtttta cttcgtcggc
      301 tccttttatt cgggcggtgt ccgatacgtt atcgctgttt cgccgagatt atcccgatgt
      361 ccatttacaa acccgcgaaa tgaacactcg cgagcaaatc gctccgctca ttgaaggaac
      421 gctggatatg ggattgctgc gtaacacagc gttaccggag acgcttgaac acgcagtcat
      481 cgtccatgaa ccgcttatgg cgatgatccc gcacgatcat cccctggcaa ataacccgaa
      541 tgtaacgctg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc acgtcgggac
      601 agggctgtat gacgatattc tcgggctgat gcgacgttac catttgacgc ccgtcatcac
      661 tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt tccgccggtc tgggtgtttc
      721 aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg tgccgattgc
      781 tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg aacaaagtcc
      841 agctgcgcgt aactttcgta ttcatctgct gaatgctctc aggtgaggga aatttcagcg
      901 aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga cgacacgtat
      961 tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag ggagtatgcg
     1021 gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa cgcgggcgcg
     1081 gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc ccgtctggcg
     1141 cagctggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc acacctggtg
     1201 caagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg gctggtggtt
     1261 gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat tttccttgct
     1321 ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc gttaaaagat
     1381 gactcgccat tgctggatcg tatcatttcc catatcgatc agttttttat ccgccaccag
     1441 aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc cgggaattat tgatacggaa
     1501 aatggcattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc gctcggcgag
     1561 gctctggagc agcataccgg cgttccggtt tatattcagc atgatatcag cgcatggacg
     1621 atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca ggtggttatc
     1681 gatcacaacg tgggggcggg cgtcattacc gatggtcatc tgctacacgc aggcagcagt
     1741 agcctcgtgg aaataggtca cacacaggta gacccatatg gtaaacgctg ttattgcggg
     1801 aaccacggct gcctcgaaac catcgccagt gtggacagta ttcttgagct ggcacagctg
     1861 cgtctcaatc aatccatgag ctcgatgtta catggacagc cattaaccgt ggactcattg
     1921 tgtcaggcgg cattgcgtgg cgatctactg gcaaaagaca tcattaccgg ggtaggcgcg
     1981 catgtcgggc gcattcttgc catcatggtg aatttattta acccacaaaa aatactgatt
     2041 ggctcaccgt taagtaaagc tgcagatatc ctcttcccgg tcatctcaga cagcatccgt
     2101 cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca gttttctaac
     2161 cagggtacga tggcaggcgc tgcgctggta aaagacgcga tgtataacgg ttctttgttg
     2221 attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc gctatctcaa
     2281 tttgggccag gaaagcataa cttagacttt caaggttaat tattttcctg gtttatattt
     2341 gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg gtacagacac
     2401 ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcat cccagggaaa
     2461 aacggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg aagggctgcg
     2521 taataaagat gccctggtgt tgcagagtgt ttcaaccatc gaactgcctt atgaagcagt
     2581 taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc caatcaatta
     2641 caccctcatt tcaaacggcc tggcaaacct gaccgaaaaa gtcgatcatg tcgtggtaga
     2701 agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccactctctg aatgggtggt
     2761 gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca ttaaccatgc
     2821 actgctaaca gctcaggcga ttgccaacga cgggctgccg ctcattggct gggtggctaa
     2881 ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctcg gtaaaaaact
     2941 tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc gtgaactggg
     3001 gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg gtagatagag tcacggtgta
     3061 acgtccgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg agtagctgat
     3121 actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc gtggttgccg
     3181 ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt aatttcttcg ccatcaggga
     3241 accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata aatagcgtcg
     3301 gtgtaaagac accaccgggc gcgccggaac cactactcgc cagcacggca cacagtttac
     3361 agaggaagat cccggcaatg atcattaaca gtggcggggc ggttaaaaag gattgtacgg
     3421 tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc acgattaagc
     3481 cgcccagtgc cagttgccag ggcggcgcaa gcttgagact tacaaatcca cgatgacagg
     3541 cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct gtactgataa
     3601 tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgctgtag agtaacgcgt
     3661 cgctatgatt aatcagattg ctaaccagca atgccacgac ggcggaaata atcaccgggc
     3721 cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa ctgccagcaa
     3781 gcggggcacg ataggccgca gccattcccg ccgcggcccc acaggcgatc cataatttcc
     3841 actcctggcg tggcgtaaaa cgttgggcaa aacaggaggc ggcaagggca gctaaaagaa
     3901 tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagcaga gaggcaagcg
     3961 atttaaccag gcttgctgcg taatcaaact gaccctcggt ttgcaacgct tccatataat
     4021 cggtcggcgc atgagggcgt tgctgggtaa atttctgcca gcccatcagc aacaaacccg
     4081 ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca cggggaaaga tttgtagctg
     4141 cattgaccag actgcccgag tcattattga ggaacaacca ctccagtagc agcatcgcat
     4201 gacgaaaccc agcaacggca aaggccgcga gaataccgac gactgttgcg ataagcagac
     4261 ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc agaaatgggc
     4321 aatgaaacac gggcgactat tttgcggcaa aagaggccag ccgcaaaagc agtgcgtgct
     4381 ttaagagata aaaaaacgtg acactgtaac gtattattgc cgaatcgcgc gtaatcgtca
     4441 ccatccggca atattacggt gatcctatcg aaacagcggt ttaaccgcga caggaatcaa
     4501 cagttgtgat tgccattgtg ccagcgttaa gcgagccagt tcacccagag cacggtagaa
     4561 agggtgttct gctttttcga taaaaacatc aagaaaacgt gttgaccacg gaaaaaggtg
     4621 ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc acgcagccat
     4681 caacagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcaa actgaattcc
     4741 tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca cagattcgcg
     4801 atccagccag accgaccccc acggcggaga cggcagtgcc cacgggccga caaacaaacg
     4861 ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga aaagccgtca ccagcggcgc
     4921 taatgacgct tctggtagag gccactgagt ttcccagccg tcactggtga gtaccgcaac
     4981 cagaggtgcg gcttcagcac tctctggagt gtaataaaac agcgcgccca acacgcgcgc
     5041 cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc tgaaatgtgc
     5101 gggttgcccc gcaccaattt gttaacctgc aatagccatt cctaccgtca tgtgcaggcc
     5161 ataaaagagc acacgcccga tcatctcacc gccaagaatg aggattaacc ccagaataag
     5221 tccggcaacg tgaggttcac ggcgacgtat gagcggacaa agccagcatc ctagacctgc
     5281 gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca gtgcgctggc
     5341 ttgttgcacg gatgagtgaa tagacgacag agacaaacct tgcagcacaa tcaccccggc
     5401 acaagcgatc agcgccagaa cgctaatgat ggcaaatggt atggtattaa atgtaacgcg
     5461 ggctgcccgc agaattgcgg cagcaagtat tggaccactc aacaataccg tcaggaagaa
     5521 agccagcgta gtgtaaccgt tatgccaggt tggcacagtg tcgatttgat acacgcaggt
     5581 catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga gtttccccaa
     5641 cgcttgcggc attttaccga tgaccgccac cagccaccat agtccgccaa cggcaaaaaa
     5701 tatggaaccg gcagcaatct cgttactcag accagaagct ccgattcgat tcagcgagtt
     5761 aaacgcccgc agtggtgatc ccaggtgcat gacagaggca ataaacccga tgcccatcaa
     5821 cagccagagg aaaaacatgc cacgaacaat gtgctgacgg tctgcgtcag ttttggcagc
     5881 gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca atacggtaaa
     5941 gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc ggattagcca
     6001 gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt aggtttgata acgatattgg
     6061 gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc aagagtgccg tgcttctggc
     6121 gcagctcttc aattggaccg aactcaagtg cacgcagcgg gcaggattcg acacatatgg
     6181 gttgtttccc ctcggcgacg cgcaaataac aaccatcgca cttcgtcatg tgcccttttt
     6241 cagcattgta ctgtggcgcg ccgtacggac aagccatgtg gcagtagcgg cagccgatac
     6301 aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg ccgctggggc
     6361 agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga taataagcga
     6421 acacgttctg gtgccagacg ccattatctt cctgccagtc gccgccagca tattcataaa
     6481 tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc agttcgcagg
     6541 ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg gtcatgggct
     6601 actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc ctttgccagc
     6661 ggtgaagggc ggtgagaggt cagaatatta atactgccgc cgtgatcgac ccggtcacca
     6721 aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg cagaatacgc
     6781 ggagtcactt ttgcggcaat cagcatttct ccattattgt taaatacccg cacggtatcg
     6841 ccatgacgga taccgcgagc ctgagcatca atggggttga tccacacctc ttgtgggcag
     6901 gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt gtagtggaag
     6961 cccgttaact gcagtggata ggttttccgc aggggatcgt cccaaccatc aaaacctggg
     7021 gtatacgcag gaaggggatg aataatttca tcttttttca attcccaggt atctgcaatc
     7081 ttcgccagtc gttcagaata aatttcgatt ttccccgaag gtgttttcaa cgggtttgcc
     7141 tgtggatctt cactgaatgc gcggaaagcg acgtagtgtt cttccgggca ttttttctta
     7201 aagatcccgg tcgttttcat ctcctcgtag tcgggcatct cagggttacg ttccttcgtt
     7261 ttcgcatgga gatatttgat ccattcatgc tgactgcgac cttcagtaaa ggtttgataa
     7321 acgtccggtc ctaagcgttt ggcgacttca ctcagcatcc agtagatggg tttgcgttca
     7381 aattttgctg aggttgcggg ttgggcgagg atcacatagc ccatattccc tgcagattca
     7441 tgagagataa ggtcttcttg ctctgttggc atcaggtcgg gcaacaggat atcgcaatac
     7501 ttagccgagg ccgtcatgaa gtggtcaatg ccaacaatca tctcgc
//
LOCUS       DAZUGK010000206         7544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZUGK010000206 DAZUGK010000000
VERSION     DAZUGK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.207"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            113..1510
                     /gene="ulaA"
                     /locus_tag="ACHY5W_003958"
     CDS             113..1510
                     /gene="ulaA"
                     /locus_tag="ACHY5W_003958"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4448745.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSIMAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1526..1831
                     /gene="ulaB"
                     /locus_tag="ACHY5W_003959"
     CDS             1526..1831
                     /gene="ulaB"
                     /locus_tag="ACHY5W_003959"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFS4448746.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1841..2305
                     /gene="ulaC"
                     /locus_tag="ACHY5W_003960"
     CDS             1841..2305
                     /gene="ulaC"
                     /locus_tag="ACHY5W_003960"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFS4448747.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2319..2969
                     /gene="ulaD"
                     /locus_tag="ACHY5W_003961"
     CDS             2319..2969
                     /gene="ulaD"
                     /locus_tag="ACHY5W_003961"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4448748.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWCDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2979..3833
                     /gene="ulaE"
                     /locus_tag="ACHY5W_003962"
     CDS             2979..3833
                     /gene="ulaE"
                     /locus_tag="ACHY5W_003962"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFS4448749.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKKVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVETA"
     gene            3833..4519
                     /gene="ulaF"
                     /locus_tag="ACHY5W_003963"
     CDS             3833..4519
                     /gene="ulaF"
                     /locus_tag="ACHY5W_003963"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFS4448750.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGAYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVHQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4648..4923)
                     /gene="yjfY"
                     /locus_tag="ACHY5W_003964"
     CDS             complement(4648..4923)
                     /gene="yjfY"
                     /locus_tag="ACHY5W_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFS4448751.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5250..5645
                     /gene="rpsF"
                     /locus_tag="ACHY5W_003965"
     CDS             5250..5645
                     /gene="rpsF"
                     /locus_tag="ACHY5W_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4448752.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5652..5966
                     /gene="priB"
                     /locus_tag="ACHY5W_003966"
     CDS             5652..5966
                     /gene="priB"
                     /locus_tag="ACHY5W_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFS4448753.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5971..6198
                     /gene="rpsR"
                     /locus_tag="ACHY5W_003967"
     CDS             5971..6198
                     /gene="rpsR"
                     /locus_tag="ACHY5W_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4448754.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6240..6689
                     /gene="rplI"
                     /locus_tag="ACHY5W_003968"
     CDS             6240..6689
                     /gene="rplI"
                     /locus_tag="ACHY5W_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4448755.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6760..>7544)
                     /locus_tag="ACHY5W_003969"
     CDS             complement(6760..>7544)
                     /locus_tag="ACHY5W_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS4448756.1"
                     /translation="TTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDHNCDG
                     RRTVEIHKLDLSDWQAFNKLAKKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAY
                     AGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPN
                     CWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSSAAIY
                     SGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1801 a         1849 c         2057 g         1837 t
ORIGIN      
        1 cttcacattt aatcataaat aatcttgttg tgattacttt tgaaaattag agtgagtgca
       61 caacattccg ggtgtgtgga atacccggtt acctcttctt caggagatcg ttatggagat
      121 cctctacaac atctttaccg tgttttttaa ccaggtcatg accaatgccc cgttgttgct
      181 gggtattgtg acctgtctgg gctacatcct actgcgcaaa agtgtcagcg ttattattaa
      241 aggcacgatt aaaaccataa ttggtttcat gttgttgcag gcagggtccg gcatcctcac
      301 cagcaccttc aaaccggtgg tggcgaaaat gtccgaagtc tacggcatta acggcgcaat
      361 ttccgatacc tacgcttcaa tgatggcaac catcgaccgc atgggcgatg cctatagctg
      421 ggtgggttac gccgtattgt tagcgctggc gctgaacatc tgttacgtgc tgttgcgtcg
      481 cattaccggc attcgcacaa tcatgttgac cggccacatc atgttccagc aggccgggtt
      541 gattgccgtt acgctgttta tcttcggcta ctccatgtgg accaccatta tctgtaccgc
      601 gattctggtt tcgctctact ggggcatcac ttccaacatg atgtacaagc cgactcagga
      661 agtgacggat ggctgtggtt tctccatcgg tcaccagcag cagtttgcat catggattgc
      721 ctataaagtc gcgccgttcc tcggcaaaaa agaggagagc gttgaagacc tcaaattgcc
      781 gggctggctg aacattttcc acgacaacat cgtctccacg gcgattgtga tgaccatctt
      841 ctttggcgcc attctgctct ccttcggtat cgacaccgtg caggcgatgg cgggcaaagt
      901 gcactggacg gtctacatcc tgcaaaccgg tttctccttc gcggtggcga tcttcatcat
      961 cactcagggc gtgcgcatgt tcgtggcgga actctctgaa gcatttaacg gtatctccca
     1021 gcgcctgatc ccaggtgcgg ttctggcgat tgactgtgcg gctatctata gcttcgcgcc
     1081 gaacgccgtg gtctggggct ttatgtgggg caccatcggt cagctgattg cagttggcat
     1141 cctggtcgcc tgcggctctt ccattctgat tattccaggc tttatcccga tgttcttctc
     1201 caacgccacc atcggcgtgt tcgctaacca cttcggcggc tggcgtgcag cgctgaagat
     1261 ttgtctggtg atgggaatga tcgaaatctt tggttgcgtc tgggcggtga aactcaccgg
     1321 tatgagtgcc tggatgggca tggcggactg gtcgattatg gcaccgccga tgatgcaagg
     1381 cttcttctcc atcggtatcg cctttatggc cgtcatcatt gtaattgcac tggcttatat
     1441 gttcttcgct ggccgcgcgc tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca
     1501 gtctgcttaa taaggagttt tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg
     1561 acaaggcagt tccatgatca tgaagatgaa agtggaccag tttttaaccc aatcaaacat
     1621 tgaccatacg gtaaacagct gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga
     1681 tatcatcatc gcttctacgc acattgcggg cgaaatcacc gtgaccggca acaaatacgt
     1741 ggttggcgtg cgcaacatgc tctctcctgc tgactttggc ccgaaactgc tggaagtgat
     1801 caaagagcat ttcccgcagg atgtgaagta aggacgcgcc atgaaattac gtgattcgct
     1861 ggcggaaaat aaatccatcc gcctgcaggc tgaagcagag acatggcagg acgcagtgaa
     1921 aatcggcgtc gatctgctgg tggcggcgga cgttgtcgag ccgcgctatt accaggcgat
     1981 tctggatggc gtggaacagt ttggccctta tttcgttatc gctccggggc tggcgatgcc
     2041 gcacgggcgg ccggaagagg gtgttaagaa aaccggtttc tctctggtaa cgctgaaaaa
     2101 gccgctggaa tttaaccacg acgacaacga tccggtggat atcctcatca ccatggcggc
     2161 ggtcgatgcc aatactcacc aggaagtggg catcatgcag atcgtcaacc tgtttgaaga
     2221 tgaagagaat tttgaccgtt tacgcgcctg ccgtaccgag caggaagtac tggatctcat
     2281 tgaccgcacc aacgcggcag cttaagaagg aattgaacat gtcattaccg atgttgcaag
     2341 tcgcgctgga caaccagact atggatagcg cctacgaaac cactcgcctg attgccgaag
     2401 aagtcgacat tatcgaagtg ggcaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc
     2461 gcgatctgaa agcgctttac ccgcacaaaa tcgtactggc agacgccaaa attgccgatg
     2521 caggcaaaat cctttcgcgt atgtgcttcg aagccaacgc tgactgggtg acggtaattt
     2581 gctgtgcgga tatcaacacc gccaaaggcg cgctggacgt ggcaaaagag tttaacggcg
     2641 acgtgcagat cgaactgacc ggttactgga cctgggaaca ggcgcaacag tggtgcgatg
     2701 caggcattca gcaggtggtt tatcaccgca gccgtgacgc gcaggccgca ggcgtggcgt
     2761 ggggcgaagc ggacatcacc gcgatcaaac gtctttccga tatgggcttc aaagtcaccg
     2821 tcaccggagg cctggcgctg gaagatctgc cgctgttcaa gggtattccg attcacgtct
     2881 ttatcgcggg ccgtagtatc cgtgatgccg cttctccggt ggaagccgca cgtcagttca
     2941 aacgttccat cgctgaactg tggggctaag gagcggatat gttgtccaaa caaatcccgc
     3001 ttggcatcta tgaaaaagcg ctccccgccg gggagtgctg gctggaacgc ctgcaactgg
     3061 caaaaacgtt gggcttcgat tttgtcgaga tgtcggtaga tgaaacggac gagcgcctgt
     3121 cgcgcctcga ctggagccgc gagcagcgtc tggcgctggt caatgcgatt gttgaaaccg
     3181 gcgtgcgcgt gccgtccatg tgcctttctg ctcatcgtcg tttcccgctg ggcagtgaag
     3241 atgacgcggt gcgggcgcag gggctggaga ttatgcgtaa agctatccag ttcgcccagg
     3301 atgtcggtat tcgcgtgatc cagctggcgg gctatgacgt ttactatcag gaagccaata
     3361 acgaaacgcg tcgtcgtttc cgtgacggcc tgaaagagag cgttgagatg gcaagccgcg
     3421 cgcaggtgac gctggcgatg gagatcatgg attatccgtt gatgaactcc atcagcaagg
     3481 cgctgggcta cgcgcactat ctcaacaatc cgtggttcca gctctacccg gatatcggca
     3541 acctgtcggc gtgggacaac gatgtgcaga tggagttgca ggccggaatc gggcatatcg
     3601 tcgcggtaca tgtgaaagac accaaacctg gcgtcttcaa aaaagtgccg tttggcgaag
     3661 gtgtagtgga tttcgaacgt tgtttcgaaa cgctcaaaca gagtggctat tgcgggccgt
     3721 acctgattga gatgtggagc gaaacggcgg aagacccggc ggcagaagtg gcgaaagcgc
     3781 gtgattgggt gaaagcgcgc atggcgaaag cgggcatggt ggagacggca taatgcaaaa
     3841 gctaaaacag caggtatttg aagccaacat ggagctgccg cgctacgggc tggtgacctt
     3901 tacctggggc aacgtcagcg ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag
     3961 cggcgttgcc tacgaaacca tgaaagcggc cgatatggtg gtggttgata tgagcggcaa
     4021 ggtggtggaa ggggcgtatc gcccatcttc cgacactgcg acgcatctcg aactctaccg
     4081 tcgttacccg tcgcttggcg gcattgtcca tacccactcc actcatgcca ccgcatgggc
     4141 gcaggcgggg ctggcgatcc cggcgttagg caccacgcac gccgactact tctttggcga
     4201 cattccgtgt acgcgcgggt taagcgaaga agaggtgcag ggcgagtatg aactgaacac
     4261 cggcaaagtg attatcgaaa cgctgggcaa cgccgagccg ctgcatacgc cgggaattgt
     4321 ggtgcatcag cacggaccgt tcgcctgggg gaaagatgcc cacgatgcgg tgcataacgc
     4381 ggtggtgatg gaagaagtgg cgaaaatggc gtggattgcg cgcggtatta acccacaact
     4441 caatcacatc gacagcttcc tgatgaataa acacttcatg cgtaaacacg gtcctaacgc
     4501 ttattacggg cagaagtaaa acacgcgctg cggaaatttc cttcttcggg agataactgg
     4561 tctaattccg cagccgtttt tcaaaaaaag ccccctgcga agggggcaaa gcaaactatg
     4621 gcaatgtttc gttggttata cctggtgcta gcgataaata tccgcgctgg cgtgcatatt
     4681 gccgttactc cccggttccc gcatcagaat tacgtggtag tacgttgcgc cttgcgcatc
     4741 tgtttcttca tttaatgcct gacgcgcttc gctttcagtg gcgaaattat gattgatata
     4801 aatcacgcct aagctttgca catcgtccat atttctggcc tggtggttat taatttcaat
     4861 ggctgcccat gtatttgcac ttagcaaaag cacagccaga agggctaaaa ctcgactgaa
     4921 catagatacc tcctcgacgg ctgactttgt gtgctctcct ctgtgatgat cttctgattt
     4981 aattttaatc aatgataaag aagttgatgg tgaccatttc tgatgcagtt gttcgaaaaa
     5041 acaccatgat gaagtgtgat gaactccaaa tcagcgtgtt agtccacttt ggtgaaagat
     5101 gagatttatt tgtcggctgg ccttgagttt agcggggtat gtcagtacta taacgcgtca
     5161 tttttcagcc gacctttaac acgttccttg cctccccggg attcggctga cccagacagg
     5221 aggctgaata atccgtaagg agcaattcga tgcgtcatta cgaaatcgtt tttatggtcc
     5281 atcctgacca gagcgaacag gttccgggca tgatcgagcg ctacactgct gccatcactg
     5341 gtgcagaagg caagatccac cgtctggaag actggggccg ccgtcagctg gcttacccga
     5401 tcaacaaact gcacaaagca cactacgttc tgatgaacgt tgaagctccg caggaagtga
     5461 tcgatgagct ggaaactacc ttccgcttca acgatgccgt tatccgcagc atggttatgc
     5521 gtaccaagca cgctgttacc gaagcatctc cgatggttaa agcgaaagac gagcgccgtg
     5581 agcgtcgcga tgatttcgca aacgaaaccg ctgatgatgc tgaagctggg gattctgaag
     5641 agtaatttct gatgaccaac cgtctggtgt tgtccggcac cgtgtgcagg actccccttc
     5701 gaaaggtcag tccatcagga attcctcact gccagttcgt gcttgagcat cgttctgtgc
     5761 aggaggaagc cggctttcac cggcaggcat ggtgtcaaat gcccgttatt gttagcggac
     5821 acgaaaacca ggccattact cacagtataa cggtcggcag tcgcataacc gttcaggggt
     5881 tcatttcatg ccacaaggca aagaacggac tgagcaaaat ggttttgcat gccgagcaga
     5941 ttgaattgat agattctgga gactagccat atggcacgtt atttccgtcg tcgcaagttc
     6001 tgccgtttca ccgcggaagg cgttcaagag atcgactata aagatatcgc tacgctgaaa
     6061 aactacatca ccgaaagcgg taagattgtc ccaagccgta tcaccggtac ccgtgcaaaa
     6121 taccagcgtc agctggctcg cgctatcaaa cgcgctcgct acctgtccct gctgccgtac
     6181 actgatcgcc atcagtaatc ggtcacggtc cattaatacg actttgagag gataaggtaa
     6241 tgcaagttat tctgcttgat aaagtagcaa acctgggtag cctgggtgat caggtaaacg
     6301 ttaaagcggg ctatgctcgt aacttcctgg taccacaggg taaagctgtt ccagctacca
     6361 agaaaaacat tgaattcttc gaagcacgtc gcgctgaact ggaagctaaa ctggctgaag
     6421 ttctggcagc tgctaatgct cgcgctgaga aaatcaatgc actggaaact gttaccatcg
     6481 cgtctaaagc tggcgacgaa ggtaaactgt tcggttccat cggtactcgc gacatcgctg
     6541 acgctgtaac tgcagctggc gttgaagtgg ctaagagcga agttcgtctg ccgaacggcg
     6601 ttctgcgtac cactggcgaa cacgaagtga gcttccaggt tcacagcgaa gtattcgcga
     6661 aagtgatcgt aaacgtagta gctgaataat tcgttattca acgagacgta aaaacgccga
     6721 ccactggtcg gcgttttgct ttctattttt cgtcaggtat tagtttcgca agtagatcca
     6781 ttcaccattc tcttgtgtcc ataagtcagg atggtttgct ggcgttagat acatacgagc
     6841 gcaactgatg tctcgctcct gcggcactcg ctcgaaaccc aaacccgagt agattgcggc
     6901 tgaacttgat aaggaacgca gaccaatttt caaatcatgg ggtaaatcag aatacgcgtt
     6961 tagcaatgca gcaatgatga atccgagggc attagaaacg cctgtaacag tgcgaagttg
     7021 gtcgatataa agatcgattg ttggtagttc agatgaccag caattaggca tttgggcagg
     7081 aagggttaca acgtatccta tcgtttggcc ggtatagtct ggagtaccat aaccaggtcc
     7141 caaaaccact tcgtgtattg tgtatttact gttgggttcg aaggattcga catcttgcaa
     7201 taaatatttc agttctgcgg ttaacctcgc acccgcgtaa gctttattgt atttctgggc
     7261 gtgaatatta tctaatgtag cttcatggtt ccatccaaaa gaattatcac cattcattgt
     7321 tactccatcg taagcattac acttctttgc taacttattg aatgcttgcc agtctgagag
     7381 atctagtttg tggatctcaa cagtacgtct gccatcgcaa ttatgatctc ttccacgatt
     7441 tgacagtaat gtagcgcgtg cgtattgatg aaattttagt tttgctggtt tgtcggtgga
     7501 cgaaaaacga ggtaaatatg cggggaatga ataaatagtt gttg
//
LOCUS       DAZUGK010000207         7509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZUGK010000207 DAZUGK010000000
VERSION     DAZUGK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.208"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..235
                     /locus_tag="ACHY5W_003970"
     CDS             68..235
                     /locus_tag="ACHY5W_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448757.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            275..493
                     /locus_tag="ACHY5W_003971"
     CDS             275..493
                     /locus_tag="ACHY5W_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4448758.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            471..1541
                     /locus_tag="ACHY5W_003972"
     CDS             471..1541
                     /locus_tag="ACHY5W_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4448759.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            1676..2959
                     /locus_tag="ACHY5W_003973"
     CDS             1676..2959
                     /locus_tag="ACHY5W_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFS4448760.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQMGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3100..5361)
                     /locus_tag="ACHY5W_003974"
     CDS             complement(3100..5361)
                     /locus_tag="ACHY5W_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFS4448761.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMKRFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNGTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5544..6977)
                     /locus_tag="ACHY5W_003975"
     CDS             complement(5544..6977)
                     /locus_tag="ACHY5W_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFS4448762.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(7053..>7509)
                     /locus_tag="ACHY5W_003976"
     CDS             complement(7053..>7509)
                     /locus_tag="ACHY5W_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HFS4448763.1"
                     /translation="IIPAEYLGKTGYELPAELDADKALLARIESIRLQAGKTMGLGDV
                     SNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAIAISSSCALEGTVTRQIVP
                     SVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRTTRKIFSGEVYLP"
BASE COUNT         2033 a         1892 c         1812 g         1772 t
ORIGIN      
        1 ctcgcactga cgaggatttc ttttatctga actcgctacg gcgggttttg ttttatggag
       61 atgataaatg cacttccgag tcacaggtga atggaatgga gaaccattca acagagttat
      121 cgaagcagag aacatcaatg actgctatga ccactggatg atatgggcgc agatagcaca
      181 tgcagacata accaatattc gaattgaaga actgaaagaa caccaagccg cctgatggcg
      241 gctttttctt gcgtgtaatt gcggagactt tgcgatgtac ttgacacttc aggagtggaa
      301 cgcacgccag cgacgcccaa gaagccttga aacagttcgt cgatgggtgc gcgaatgcag
      361 gatattccct cctccggtta aggatggaag agagtatctg ttccacgaat cagcggtaaa
      421 ggttgactta aatcgaccag taacaggtag ccttttgaag aggatcagaa atgggaagaa
      481 ggcgaagtca tgagcgccgg gatttacccc ctaaccttta tataagaaac aatggatatt
      541 actgctacag ggacccaagg acgggtaaag agtttggttt aggccgagac aggaggatag
      601 caatcactga agcaatacag gccaatattg agttattttc aggacacaaa cacaagcctc
      661 tgacagcgag aatcaacagt gataattctg ttacgttaca ttcatggctt gatcgctacg
      721 aaaaaatcct cgccagcaga ggaatcaagc agaagacact cataaattac atgagcaaaa
      781 ttaaagcaat aaggaggggg ctgcctgatg ctccacttga agacatcacc acaaaagaaa
      841 ttgcggcaat gctcaatgga tacatagacg agggcaaggc ggcgtcagcc aagttaatca
      901 gatcaacact gagcgatgca ttccgagagg caatagctga aggccatata acaacaaacc
      961 cggtcgctgc cactcgcgca gcaaaatcag aggtaaggag atcaagactt acggctgacg
     1021 aatacctgaa aatttatcaa gcagcagaat catcaccatg ttggctcaga cttgcaatgg
     1081 aactggctgt tgttaccggg cagcgagttg gtgatttatg cgaaatgaag tggtctgata
     1141 tcgtagatgg atatctttat gtcgaacaaa gcaaaacagg cgtaaaaatt gccatcccta
     1201 caacattgca tgttgatgct ctcgggatat caatgaagga aacacttgat aaatgcaaag
     1261 agattcttgg cggagaaacc ataattgcat ctactcgtcg tgaaccgctt tcatccggca
     1321 cagtatcaag gtattttatg cgcgcacgaa aagcatcagg tctttccttc gaaggggatc
     1381 cgcctacctt tcacgagttg cgcagtttgt ctgcaagact ctatgagaag cagataagcg
     1441 ataagtttgc tcaacatctt ctcgggcata agtcggacac catggcatca cagtatcgtg
     1501 atgacagagg cagggagtgg gacaaaattg aaatcaaata atgattttat tttgactgat
     1561 agtgacctgt tcgttgcaac aaattgataa gcaatgcttt tttataatgc caacttagta
     1621 taaaaaagca ggcttcaacg gattcatttt tctatttcat agcccggagc aacctgtgaa
     1681 cacattttca gtttcccgtc tggcgctggc attggctttt ggcgtgacgc tgaccgcctg
     1741 tagctcaaca ccacccgatc aacgtccttc tgatcaaact gcgcctggta cctcttctcg
     1801 cccgattctg tcggcaaaag aagcgcagaa tttcgatgcg caacactatt ttgcatccct
     1861 gacaccaggt gctgcagcgt ggaatccgtc cccgataacc ctgcctgcgc aacctgactt
     1921 tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc acgattcagg cggcggtaga
     1981 tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc ctggtgagta
     2041 tcagggaacg gtgtatgtcc ctgctgctcc aggtggaatt actctgtacg gtacaggtga
     2101 aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg ggcatgagcc ctgccgactg
     2161 gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt aaaccagcgt ggtatatgta
     2221 cgatagctgc cagagcaaac gcagcgacag tatcggtgtt ctctgttctg cggtcttctg
     2281 gtcacaaaac aatggcctgc aactgcaaaa tctgaccatc gaaaacacgc tgggcgatag
     2341 cgtagatgca ggtaaccatc cggcggtggc actgcgtact gacggtgacc aggtacagat
     2401 taacaacgtt aacattctcg gtcgtcagaa caccttcttt gtcaccaaca gcggtgtgca
     2461 gaaccgtctg gaaacgaatc gtcagccgcg tacgctggtg accaacagct acattgaagg
     2521 ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc gataacaccg aattccgcgt
     2581 ggtgaactca cgtactcagc aagaagcgta tgtgtttgca ccggctacgc tgtccaacat
     2641 ttactacggt ttcctcgccg taaacagccg tttcaatgct tccggtgatg gcgtggcaca
     2701 aatgggccgc tcgctggatg ttgatgccaa taccaacggt caggtggtga tccgtgatag
     2761 cgccatcaac gaaggtttta acacggctaa accatgggcc gatgcggtga tctctaatcg
     2821 tccgtttgcg ggtaataccg gcagcgttga cgatagcgac gaaatacagc gcaatctgaa
     2881 tgacactaac tacaaccgca tgtgggaata caataaccgc ggcgtgggta gcaaagtggt
     2941 tgcagaggcg aagaagtaag agctattaac tatttgccgg atgcggcgta aacgccttat
     3001 ccggcctacg gttcgatgcg atttgtaggt cggataagat gcgccagcat cgcatccgac
     3061 aataagtgcc gaatgcgacc tacattcaca tggtgctttt tacatctgac ggtttttatt
     3121 gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca gactttccat
     3181 atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt taaacgtcgt
     3241 acccggatta tccagcgccg ctttaatacc agggatgtaa atgtaatccc ccacttcaaa
     3301 ggttggcact tccgccatct gcaaaggtaa catcccccag ttgatgacgt tagaacggta
     3361 gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac gctggcagct
     3421 cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca tgctgccaat
     3481 ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc gcgcaaacac
     3541 ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag cagtcgcttt
     3601 acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca gaccaatcgg
     3661 atttgaacga taagaagagg tttcaccgga aggaatcaat tcgtcggtgg tggtcacttc
     3721 gtcgaggatc ttcgagcaca ctttgaggac gatattgtca gtcagcgcac ccaattccgg
     3781 ccagtcttta atgttcggtc cgtaaatcag cggttgctgg gtcgcccctt tcacaaagcc
     3841 ttgatagacg cgatttttat acggcgttac atcgaaggcg tactccggca cgttgtccca
     3901 gcaatcgagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg cagcgataga
     3961 acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag agccttcgcg
     4021 gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg gcgtatcgcc
     4081 cgcgccaaag catgggccac agaacgcggt tctgataatt gcgcctgcgc caatcaaatc
     4141 tgctaccaca ccttttttgg ctaaatccat aaacaccggc tgtgatgacg ggtaaaccgc
     4201 cagcgagaag gtgccattgc cacaggattg accgcgcagt gcattcgccg ccgcgataac
     4261 gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt tcaggcggcc
     4321 attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc gctcggactc
     4381 aatttcaatc tcacgtagaa tgtcggtcag gttctggttc agcgtgtcga tttcatacac
     4441 gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat caacgctgat
     4501 gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct ggccgcgacc
     4561 gtgcagcgcc agccagttat ggacttcttc atcggtttgc cagacagaac ttaaacaggt
     4621 cgtttcagtg gtcataacgt caacgctgtt acggaaatcg gtagagagcg cagcaacacc
     4681 gggacctacg aattccatca ctttgttttt gacgtagccg tttttgaaca cggcaccgat
     4741 gatagccaac gccacatcct gcggccccac atacggcgct ggttttccgg tcagatgcac
     4801 cgcaacaact cccggatagt cgatatccca ggtgtcatta agcagctgtt ttaccaactc
     4861 accgccgccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt ggctgtctga
     4921 cccgaggatc attttgccac cgcctgccat catctcacgc atatattgat ggatgaccgc
     4981 aatgtgcgga ggcacaaaaa taccgccata acgctgagcc gccgataaac caaaaacatg
     5041 gtcatcaccg ttaatggtgc caccgacggc gcagagtgag ttatggcagt tggtcagcac
     5101 atacggcagc gggaaacgtt tcataccgga cgctttagcg gtctgtacaa taccaacaaa
     5161 ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca tatttccgga
     5221 cgtattatgc gaagagagaa tagaccaggc aatagtgcct tttttggctt cttctttttt
     5281 aatttcgccg gtgaaatgtt cttcggcaat tatttcgtta ttactggcga gaaacacgcc
     5341 tttttcagat aacttgatca tcaaatgctc cagatatctg cgcaccgtga agtgcgcaga
     5401 aagataataa atggatatac tctaaataat tcgagttgca ggaaggcgac aagcgagtga
     5461 atccccagtc acttacataa gtaagtgact ggggtgagcg aacacagacg cagcacatgc
     5521 aacttgaagt atgacgagta tatttacagc atgttccagc cgatcagcat attccaccac
     5581 cacacgccga gggtgatatg caccaggaag gttaatatcg tcagtaccgc accgaccaac
     5641 caccaggatt taatatcgtt gtaacccacg ccaaagatga ccggacctgc cgcgccgcca
     5701 tagtgagtaa ccatgccgcc ataggagttg gagaacaaca gtgccagcgc ggttaacatt
     5761 aacggtgcgc cggagacgtt cgccagcatg gcaaataccg gcaacatggc aacgatatag
     5821 gcactaccgg aagcgaagaa ataacgcacg atgatgctga ggaaaataat aacgaagaaa
     5881 gcaacgttac cgtgaccatc aaatgccagg ttatttttaa agacttcagc taaccattcg
     5941 aagaatttaa ctttcgataa taaggagctt aagccgataa taccgccgta ccagattaag
     6001 gtattccagc cgcctttatt tttaaccacg tcttcccagg taacgatacc cagcagcagc
     6061 atggtcgcca taacaacgat tgccacggtg gattcatcaa cccccaggga cttactgaaa
     6121 atccagccca gcagcgccag cacaaagaca ccgagcagca ttttttcgcg gattttcatc
     6181 ggtcccagtt cggcaaggcc cgctttggcg atggttttat tatccacctt cttaatttct
     6241 ggcggataca tggtgtaaat caccagcgga gtaaccagca gcataatgat gcccggcaat
     6301 ccggcggcca gcgcccatcc cccccagcta atttgcaggt gcagaatgtc gttgatcatt
     6361 ttcagcgcca gaatgttccc cgccattgcg gtaaagaaca tatagctggt ggttttggtg
     6421 accatgtaaa tggacatcat caggtaatgt ccgacacgac gcggactttt ttccggttcg
     6481 gaccccaaag ccaccgccac gctgttgatg atcggtaaca caatgccgcc cgcacgcgcg
     6541 gtgttagacg gtgttgccgg agccagtacc agatcgagaa ataccgtaac gtaacccagc
     6601 cccagggtag tgctaccaat tttaccaatc agcaaatagg caatacgttt acctaagccg
     6661 gtggtcacaa atgcggcgct taaggtaaac gccgagaaca ccagccaggt ggtacctgaa
     6721 gagtaaccgc ttaatacggc ggtggtctta aacgcaccgt cggataagtt accgaccacc
     6781 accatggagg cagcaacagc aattaacagt acgacaggtt ccgggaaagg cttgataacc
     6841 agccccacaa tggccgccag gtaaatacca aaaagcaccc acgccagttc gcttaatcct
     6901 gccggagctg gcataaaacc aataatacat gggatcgcta atatcagaat tagcttccat
     6961 aacgatttct tattcattta aaatcctgtt ctttctgtat gactcataca gaagtccttt
     7021 attgattgtc cttatcctga caacgaattt tttcagggaa gataaacttc accggaaaat
     7081 atttttctgg ttgtccgaat aacagatgcg cgtaacgtcg tggcatcctg accttcatta
     7141 cttaaatgaa cgtcgagcgc accactgggg tgttcaatat tgatattgcc gtatcctaca
     7201 gaagggacga tttgtcgggt gacggtgcct tccaatgcac aactactgga aatagcaata
     7261 gcaccggtta tcgccagcgc gcgatggcaa gaatgcggca taaaataacg cacattaatt
     7321 gccccgcctt tctgcgctgg agaaataagc acaggtttag ggataaccat attactgaca
     7381 tcgcctaagc ccattgtttt acccgcttgt agacggatag attcaatgcg ggctaataat
     7441 gctttgtcgg catccagttc cgccggtaat tcatagcctg tttttcccag atattcagcc
     7501 ggaataatg
//
LOCUS       DAZUGK010000208         7484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZUGK010000208 DAZUGK010000000
VERSION     DAZUGK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.209"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..1779
                     /gene="adeD"
                     /locus_tag="ACHY5W_003977"
     CDS             13..1779
                     /gene="adeD"
                     /locus_tag="ACHY5W_003977"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4448764.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1825..3216)
                     /gene="uhpT"
                     /locus_tag="ACHY5W_003978"
     CDS             complement(1825..3216)
                     /gene="uhpT"
                     /locus_tag="ACHY5W_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFS4448765.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3354..4673)
                     /gene="uhpC"
                     /locus_tag="ACHY5W_003979"
     CDS             complement(3354..4673)
                     /gene="uhpC"
                     /locus_tag="ACHY5W_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS4448766.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4683..6185)
                     /gene="uhpB"
                     /locus_tag="ACHY5W_003980"
     CDS             complement(4683..6185)
                     /gene="uhpB"
                     /locus_tag="ACHY5W_003980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFS4448767.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6185..6775)
                     /gene="uhpA"
                     /locus_tag="ACHY5W_003981"
     CDS             complement(6185..6775)
                     /gene="uhpA"
                     /locus_tag="ACHY5W_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFS4448768.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6937..>7484)
                     /locus_tag="ACHY5W_003982"
     CDS             complement(6937..>7484)
                     /locus_tag="ACHY5W_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448769.1"
                     /translation="EIFKKVVFTPGECDLVRDSLVQDGIKPEKIAKIISCLSSRTGIR
                     NLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFT
                     QTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWAR
                     DNDIRNLRRNFSGNITIATYK"
BASE COUNT         1917 a         2106 c         1906 g         1555 t
ORIGIN      
        1 aatgagaaaa atatgaataa ttccattaac cataaatttc atcacattag ccgggcggaa
       61 taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat tattgataat
      121 gtctctattc tcgacctgat taatggcgga gaaatttccg gcccaattgt gattaaaggg
      181 cgttacattg ccggtgttgg cgcagaatac actgatgctc cggctttgca gcggattgat
      241 gcccacggcg caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc
      301 atgatgacgc cggtcacctt tgaaaccgcc accctgcctc gcggcctgac aactgttatt
      361 tgcgatcctc atgaaatcgt caacgtgatg ggcgaagccg gtttcgcctg gtttgcccgc
      421 tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt acccgctctg
      481 gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc ctggcgggac
      541 catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat tagcgggcag
      601 aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg tcactgcccg
      661 ggtttgggtg gtaaagaact taacgcctat attgctgcgg gtattgaaaa ctgccacgaa
      721 agttatcagc tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt gatgatccgc
      781 gaagggtccg ctgcccgcaa tctcaacgca ctggcaccgt tgatcaacga atttaacagc
      841 ccgcaatgca tgctctgtac tgatgaccgt aacccgtggg agatcgccca tgaaggacac
      901 atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccgctgca tgtggcatat
      961 cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga atcacctcgg cttactggca
     1021 cctggtaagc aggctgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag
     1081 gtactggtga aaggcgaacc gattgacgcg caaaccttac aggcggaaga gtcggcgaaa
     1141 ctggcacaat ccgctccgcc atatggcaac accattgccc gccagccagt ttccgccagc
     1201 gactttgccc tgcaatttac gcccggaaaa cgctatcggg tcattgacgt catccataac
     1261 gaattgatta cgcactccca ctccagcgtc tacagcgaaa atggttttga gcgcaatgat
     1321 gtgtgcttta ttgccgtact tgagcgttac gggcaacgtc tggctccggc ttgtggtttg
     1381 ctcggcggct ttggtctgaa tgaaggcgcg ctggctgcga cggtcagcca tgacagccat
     1441 aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tagcggtcaa tcaggtgatt
     1501 caggatggcg gcgggttgtg cgtggtacgt aacggccagg tccaaagcca tctgccgtta
     1561 cccattgccg ggctgatgag caccgacacg gcgcagtcgc tggcggagca aattgacgcc
     1621 ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat tcagatggcg
     1681 tttctttccc tgccagtgat ccccgcgctg aaactaacca gccaggggct gtttgatggc
     1741 gagaagtttg ccttcaccac gctggaagtc actgaataat aaaaaaagcc cggcgtcatg
     1801 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     1861 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     1921 ggcgatatcc agcgcagcga aggtgcctgc ccagccggta aggccgaata ctggcgtccc
     1981 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcgaaggt
     2041 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2101 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2161 gatatattcg ttgctggcat gttggtacac accgagcgtg gcgataatca gcgccagcgc
     2221 gatgcaggcc accaggccac ggcgaccgtt cgctaggtca gagagccagc cccacagcag
     2281 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2341 gagtttcagt tcctggaacg cgtacacagt tgaccactgg tcgataccaa tacgcaccac
     2401 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2461 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2521 ctcctcgccg aacagctctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2581 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2641 gatgacatgg ccatcgaaca ggtaatttgc gccgaacagt gccacaccag ctgcgcctgc
     2701 accgccaagg ttgtgagaaa tgttccagaa accgaggaac gtcccgcgtt tacgacgcgg
     2761 cgtccatttg gtgatggtgg agtaactgca cgaacccccg gtactctgga aaaagccact
     2821 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcgct
     2881 aaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     2941 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3001 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgaaatca tatcgttctg
     3061 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3121 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3181 ggtcggcttg cggacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3241 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3301 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3361 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggca
     3421 atggcgataa ccacaaaaaa tccgctccag tgccaggtat cgagcacttt cgccagcggc
     3481 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3541 gccgcctctt tgtgggaaca ttctgccgcc gccatgccga ttaacatctg cgggccaaag
     3601 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3661 agccacagcg agccgactga aagcaaaatt ccggcagcga aaatcaaatt catcggcccg
     3721 cggttgccgt taaacagttt gtccgagccc caacccgcta ccagcgcacc gataaatccg
     3781 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     3841 gacatataca aattgcccca gtcgttgatc gccgcccgaa ccacatagac cagcacatag
     3901 caaaacgaaa gcagccagat atacggattc aacaacacat acttggtgag gatctcttta
     3961 cgcgtcagcc ctgccccttc ttgttgttga gcaatctcca gcgcgtcgtg tcgccattca
     4021 ccgaccgctg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatc
     4081 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgcagc
     4141 gcagctgctg ccatcacaat gggaatgagc gcaccgccaa cgttatgcgc cgtgttccat
     4201 aatgcccacc aaccaccgcg ttcggtacga gaataccagg ccgttaacag acgcgcacac
     4261 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagcacggc aaacgcccat
     4321 agcgacgtcg agaagccaaa cagaatgttg atgatgcccg tggcgataag ccctatcccc
     4381 ataaaataac gggcatttga gcggtcgctg acaatgccgg agacaaactt cgacacgccg
     4441 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccatta
     4501 gcaaggattt ctggtacggc ggcgttaaaa cttttacgcg tgaagtaaaa cagcgcgtaa
     4561 cccagccaga tggtcagcag aatatgccga cgccagtagc gataacgggc atcaatttca
     4621 tgtttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4681 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4741 gtcaaggtgc cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     4801 tgcccggaac ccggcggtaa accgctgcca tcgtcttcaa tcaccagcat caaccgttca
     4861 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttattc
     4921 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgactct
     4981 tcgattcgcc attcgagatg gctgacaatg ccgcgccctt ccagctccat ttcccgcatc
     5041 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgccct
     5101 aacaaacggc gcaccgcgtc gtacacgccc agcgacagtt gttcgatgag ctgcccgctc
     5161 tgcttcacac tggcgttatc tgccgccagt cgctggacaa tgcccgcttg agtacgaatg
     5221 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5281 tcttcagttt ctaacaaacg ttcagcgaga tgctgattgc gcgccagttc cttttgcagc
     5341 gactggttaa gttcacgcaa ccgctggatg ccagcgccca gtaacaaccc tgtcagactt
     5401 tgcaccagca gcgagagcaa taaatccaca ggatgatcgc gccaggtttg actggcgatc
     5461 agcgcgatgg cgttcatcaa tgtcgctatc agcgcccctt gccagccgta gtgccaggcc
     5521 agcgcgataa tcggcagcgc caggcagaat ggcgtaaagc gcgacagttc gtccggcaag
     5581 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgccgcccg
     5641 cgccagttga tcggttgaga aactaacgac ggcccgagcg gcagccaggt gttattggcg
     5701 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagttaac
     5761 agcagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     5821 gccgccgtta acgccgcacc ctgtaacagc aaggtgcgcc agtcacgttg atggcgatag
     5881 cgcgagatca gcgccacggg cagtaacgtc agtaaactac cgatcatcaa taaggagaaa
     5941 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc tcccagcagc
     6001 accggccagt atccgcgcgg gcattgcagc attagcccta gccgcagacc aaacggaaat
     6061 aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca caggcaaaac
     6121 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6181 ttcatcacca gccatcaaac atacggcgcg ccagttcaac gtcgttactg acgcccagtt
     6241 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6301 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctgccgt tcacgtttgg
     6361 ttagtgggtc ctggcgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6421 cgcctgtggc aaccgtatgc accgcagcaa tcagttcgtc agggctacaa cgcttggaga
     6481 gaaagccacg cgcccccgcg ttaagcgcct gctcaaccag cgccggactg tcatgaacgg
     6541 agagcataat tgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6601 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6661 cctcgcgccc cgaaccaaac tcggcaacta cttgcaaatc aggttccagc cccagcaact
     6721 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatgattg
     6781 ggtcctggcg gataaaaaac gcgcatacct taacgataag cgcgatgttg ttcaagttgg
     6841 atatggggta tgtattggct taatgatgtt gagcttatcg tcaaaacgct tacctgagag
     6901 gtaagcgttt ggtgcgtgtt attgagaaaa agcgaattat ttgtaggttg caatcgtaat
     6961 attgcctgag aaatttctcc ttagattgcg gatatcatta tcccttgccc aataatcatc
     7021 tttgtgtcta taactctcat cggaatgtac atagggagaa tcatcttcag cgtcgttatg
     7081 gataatattt tcgcctttag tggtatagat ataattagca acatcaaagg tgtcattcgc
     7141 gttatttacg actgcgccta agacgcaaag ggtttgagtg aatccgtatt ctgagaaatc
     7201 gacatgctga tggtaatttc taccattcaa agcaacaaaa ctcggacaaa catctattgt
     7261 atcgccaaca gacgtagcgg cgcgacgcac atcaagtgca gcgttatcgt tagcaacatg
     7321 aaatgcttgt gaacaggcat aaaacaaatt tctaatcccg gtacgactgg ataaacaaga
     7381 aataattttt gctatttttt ctggctttat accatcctgt actaaagaat ctctcaccag
     7441 atcacattca ccaggtgtga aaacaacttt cttgaatatt tcct
//
LOCUS       DAZUGK010000209         7312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZUGK010000209 DAZUGK010000000
VERSION     DAZUGK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.210"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..1189)
                     /gene="argE"
                     /locus_tag="ACHY5W_003983"
     CDS             complement(38..1189)
                     /gene="argE"
                     /locus_tag="ACHY5W_003983"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFS4448770.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVHPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAEATAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1343..2347
                     /gene="argC"
                     /locus_tag="ACHY5W_003984"
     CDS             1343..2347
                     /gene="argC"
                     /locus_tag="ACHY5W_003984"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFS4448771.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            2355..3131
                     /gene="argB"
                     /locus_tag="ACHY5W_003985"
     CDS             2355..3131
                     /gene="argB"
                     /locus_tag="ACHY5W_003985"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFS4448772.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDKGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3192..4565
                     /gene="argH"
                     /locus_tag="ACHY5W_003986"
     CDS             3192..4565
                     /gene="argH"
                     /locus_tag="ACHY5W_003986"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFS4448773.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQAALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRVDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            4833..5750
                     /gene="oxyR"
                     /locus_tag="ACHY5W_003987"
     CDS             4833..5750
                     /gene="oxyR"
                     /locus_tag="ACHY5W_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFS4448774.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5733..7133)
                     /gene="sthA"
                     /locus_tag="ACHY5W_003988"
     CDS             complement(5733..7133)
                     /gene="sthA"
                     /locus_tag="ACHY5W_003988"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFS4448775.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1690 a         1803 c         2084 g         1735 t
ORIGIN      
        1 gccggatgcg gcgcaagcgc cttattcggc ctacgtttta atgccagcaa aaatggtgaa
       61 tgacctgggt tatcagttcg cgggtgggct tgataaaccg tgtttccaga tattcatcag
      121 gctgatgagc ctgattaatt gagccaggcc ccagaaccag cgtcgggcat aacgtttgaa
      181 taaacggcgc ttcggtacag tagttcacca cttcggtctt tgctccgagc aatttctcaa
      241 ccacttcaac cagttgatga tttggcgggc attcatagcc agggatcggc ggatgcagct
      301 cgtcgacagt cagacgaccc ggccagcgtt cgctcaccgg agccaatgca tcgttgagca
      361 aaccattaag ttcattgagt gtcatgccag gcagcggacg aatatccata tgcaactcac
      421 agcaagcgca aatacggtta gaagcgtcgc caccgtgaat atgcccgagg ttgagcgtag
      481 ggtatggcac ggtaaacgct tcgtagtgat aacgttcttt caggttatcg cgcaattgca
      541 aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt aactccgcgt gctggatcgc
      601 tggagtgccc cgactggccc tgaatacgga tggcgttaga gatatgacct ttatgtgcgc
      661 gtaccggttg tagtgacgtc ggttcgccaa tgatggcgca atccgggcgc agggcggtag
      721 cttcggcaaa ataacgcgct ccggccatac tggtttcttc atcagcagtc gccagaatgt
      781 agagcggttt tttcagtttc gtgacgtcga catcgcgtag cgcatcaagg ataaacgcaa
      841 aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg
      901 taaacggatc acgcgtccag cgaccgtcat caaatggtac cgtatcggta tgccccgcca
      961 gcaacaagcc gccagccccc tgtccgcaac tggccagcat attaaatttg ttgcgagttc
     1021 ctggcacagg gtgcacttcc acattgaagc ctaaatcttt aaaccagtcc gccagcagag
     1081 tgattaaatc tgcattgctt tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg
     1141 caatcagagc acggtaaatc tcgataaatg gcggtaattt gtttttcatt gttgacacac
     1201 ctctggtcat gatagtatca atatttatgc agtatttatg aataaaaaca cactaacgtt
     1261 gagcgtaata aaacccacca gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga
     1321 cataagaagg tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc
     1381 tggcgcagag ctagtgacct atgtaaatcg ccatccgcat atgaacataa ccgctttgac
     1441 tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa
     1501 aggcatcgtt gagctgccgt tgcagccgat gtcggatatc agcgagttta gcccaggggt
     1561 ggacgtggtg tttctcgcca ccgcccatga agttagccac gatttagcgc cgcagtttct
     1621 tgaagcgggc tgcgtggtgt tcgacctttc cggcgcgttt cgtgttaacg acgccacctt
     1681 ctatgaaaaa tattacggct ttacccatca atacccggaa ctgctggaac aggcagccta
     1741 cggtctggcg gagtggagcg gtaataaatt aaaagaagcg aatttgattg cggtgccagg
     1801 ctgttatccg acggcggcac agctggcgct gaaaccgttg attgatgccg atcttcttga
     1861 cctcaatcag tggccggtga tcaacgccac cagcggcgtg agtggtgcag gacgtaaggc
     1921 agcgatttca aatagttttt gtgaagttag cctgcaaccg tatggcgtct ttactcatcg
     1981 ccatcaacca gagatcgcca cacacctcgg tgctgacgtt atcttcaccc cgcatctggg
     2041 caatttcccg cgcggcattc tcgaaaccat tacctgccgc ctgaaaccgg gtgtgaccca
     2101 ggcgcaagtg gcgcaagcgt tacagcaggc gtatgcccat aaaccgctgg tgcggctgta
     2161 tgacaaaggc gttccggcgc tgaaaaatgt cgttgggcta ccattttgcg atatcgggtt
     2221 tgctgttcag ggcgaacatc tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc
     2281 ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct
     2341 tatttaaggg tgcaatgatg aatccattaa ttatcaaact gggtggcgta ctgctggata
     2401 gtgaagaggc gctggaacgt ctgtttagcg cactggtgaa ttatcgtgag tcacatcagc
     2461 gtccgctggt gattgtccac ggcggcggtt gcgtggtgga tgagctgatg aaagggctga
     2521 atctgccggt gaaaaagaaa aatggcctgc gggtgacgcc tgcggatcag atagacatta
     2581 tcaccggagc actggcggga acggcaaata aaaccctgct ggcatgggcg aagaaacatc
     2641 agattgcggc cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg acccagcttg
     2701 atgaagagtt aggtcatgtt ggactggcgc agccaggttc gcctaagctt atcaacacct
     2761 tgctggagaa cggttatctg ccggtggtca gctccattgg cgtaacagac aaagggcaac
     2821 tgatgaacgt caatgccgac caggcggcaa cggcgctggc ggcaacgctg ggcgcggatc
     2881 tgattttgct ctccgacgtc agtggcattc tcgacggcaa agggcaacgc attgccgaaa
     2941 tgaccgccgc gaaagcagaa caactgattg agcagggcat tattactgac ggcatgatag
     3001 tgaaagtgaa cgcggcgctg gatgctgcgc gcacgttggg ccgtccggta gatatcgcct
     3061 cctggcgtca tgcggagcag cttccggcac tgtttaacgg tatgccgatg ggtacgcgga
     3121 ttttagctta agttttgttg gccggaggcg cagctttccg gcattgaatt tcaaaataag
     3181 gaaacagagt tatggcactt tggggcgggc gttttaccca ggcagcagat caacggttca
     3241 aacaattcaa cgactcactg cgctttgatt accgtctggc ggagcaggat attgttggct
     3301 ctgtggcctg gtccaaagcc ctggtcacgg taggcgtgtt aaccgcagaa gagcaggcgc
     3361 aactggaaga ggcgctgaac gtgctgctgg aagatgttcg cgccaggcca caacaaatcc
     3421 ttgaaagcga cgccgaagat atccatagct gggtggaagg caaactgatc gacaaagtgg
     3481 gtcagttagg taaaaaactg cacaccgggc gtagccgtaa tgatcaggta gcgacggacc
     3541 tgaaactgtg gtgcaaggat accgttggcg agttgctgac ggctaaccgc cagctgcaag
     3601 cggcgctggt ggaaaccgcg cagaacaatc aggatgcggt aatgccaggt tacactcacc
     3661 tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgctgg
     3721 cgcgtgatga aagccgtttg caggatgcgc ttaagcgtgt ggatgtcagc ccgttaggtt
     3781 gtggcgcgct ggcgggaacg gcttatgaaa tcgaccgtga acagttagcg ggctggctgg
     3841 gatttgcttc agcaacccgt aacagtctcg acagcgtttc tgaccgtgac cacgtgctgg
     3901 aactgctgtc ggctgccgct atcggcatgg tgcatctttc gcgttttgct gaagatctga
     3961 ttttctttaa caccggcgaa gcggggtttg tggagctttc ggaccgcgtg acttccggtt
     4021 catcattaat gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg
     4081 gtcgggtgca gggcgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt
     4141 acaacaaaga tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg
     4201 actgcctgca tatggcggcg ctggtgctgg acggtattca ggtgaaacgt ccacgttgcc
     4261 aggaagcggc tcagcagggt tacgccaacg ccaccgaact ggcggattac ctggtggcga
     4321 aaggcgtacc tttccgcgag gcgcaccata tcgtgggtga agcggtggtg gaagccattc
     4381 gtcagggcaa accgctggaa gatctgccgc tcgacgagtt gcagaaattc agcccggtga
     4441 ttggcgaaga tgtctatccg attctgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa
     4501 aaggcggcgt ctcaccgcag caggtggccc aggcgattgc ttttgcgcag gcgcggttgg
     4561 ggtaagaaca tttatatgta caaatttgag cctggcttat cgccgggctt ttttatggca
     4621 aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg
     4681 agaaactctc aaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt
     4741 gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg
     4801 ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg
     4861 tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc
     4921 cgacgcttag cgggcaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc
     4981 ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta
     5041 ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt
     5101 ccggaccgct gcatattggt ttgattccca cggttggacc gtacctgcta ccgcatatta
     5161 tcccgatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     5221 accagttact ggcgcaactg gacagtggca aactcgattg cgtgatcctc gcgctggtga
     5281 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     5341 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gctggggaaa
     5401 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgttttg
     5461 aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca
     5521 tggtggcggc aggtagcggg atcactttac tgcctgcgct ggctgtgccg ccggagcgca
     5581 aacgcgatgg ggttgtttat ctgccgtgta ttaagccgga acctcgccgc actattggcc
     5641 tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc
     5701 gcgcaagaat ggatggccat ttcgataaag tgttaaaaca ggcggtttaa gccatttagc
     5761 gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac
     5821 tcaatagtgt tgccgccacc tttctgttcc ataatcgcct ggccgatatg aataatttcg
     5881 gcagcgcgct cgccaaagca gtgaatgccc agaatctctt tcgtttcccg atggaacaaa
     5941 attttcagcg tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttgaactgg
     6001 gcgcggccca cttcatatgg cactttcatc gcggtcagct gctgttcggt tttgcccaca
     6061 gagctgattt ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg
     6121 gcttcacctt tcaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgccgacgcc
     6181 aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac
     6241 atgctgttga ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc
     6301 agcgaatcgg tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt
     6361 ttacccgact tcagatgcat gatcacaccg tcgtcacagc cttcgatctt ctcgtactct
     6421 tcgttgtggc gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc
     6481 tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc
     6541 ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca
     6601 tggcgcggtt cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg
     6661 aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg
     6721 gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag
     6781 cgagcgttcc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc
     6841 gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc
     6901 agtcgggaat ggtcgctgta tagtgggttt tgattgaatt ctataatgcg gctgacggcg
     6961 tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga
     7021 taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg
     7081 ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg
     7141 cttacctgtt cttatacata aaagcaacag aatggtaaca ttttatcgcg ggtaagccaa
     7201 ttgatccccg tcattaatct ggctatagcg tgagcggcct ttgctttgtc tgcctccttc
     7261 ttaacccatt tattcactat taatcgcttt ttcttatggc tgacagtttt at
//
LOCUS       DAZUGK010000210         7291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZUGK010000210 DAZUGK010000000
VERSION     DAZUGK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.211"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1792
                     /locus_tag="ACHY5W_003989"
     CDS             <1..1792
                     /locus_tag="ACHY5W_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFS4448776.1"
                     /translation="VWKVDDQVEQFLMDLDNEYQPEQKDQNGYFLGSVIIVKNGEKHD
                     VIDGQQRLTTIVITMCALRDLLKDRLDTLDKKGLEYLKTIEEWLSSYDINSEENLIRL
                     DLQYEESRGFISRLIEGQTNTFAETASVKKMADAYDHIRDYLKYQLQDSLNGLISFAR
                     FFLTKVELVVIESENLSSALKIFETINQRGASLNAMDLVKNLIFSQIKEEQFSAIKTT
                     WKSITNNLESCGESDNPLRFLRYFLMARYHHGILREDDIYKWIISAEGELSLQYKTRP
                     MVLVKELEKLSERYANLVNATNEAAYWRENIRFPNVSNIGYINKYRSRQHLVLLMALD
                     ISCSDDVMDFLGAQLESFLFFTNTMAIQSKTYEQRFTQWAVKLRDVKTKAEVANVVSA
                     TLIPFVVERLDEFKERFSIINDYNFTPQYRQRYVLGRLENHIRHLCGFPEKSQQAIQQ
                     MQIEHILPQTVRNAPENGEFSDVNRTSYLYKLGNVTLLEGTINQSVNNCNDLSEDWFL
                     KKQTEYLNSEIIMTRLLHSQYSIGDNTALNRFKLKYGYEYQDWSAESITSRQRLLMDL
                     AIDCWRFNDQRIDAYIVNNLGTGPGNEFTL"
     gene            2391..3056
                     /gene="yecA"
                     /locus_tag="ACHY5W_003990"
     CDS             2391..3056
                     /gene="yecA"
                     /locus_tag="ACHY5W_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFS4448777.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3118..4329)
                     /gene="tyrP"
                     /locus_tag="ACHY5W_003991"
     CDS             complement(3118..4329)
                     /gene="tyrP"
                     /locus_tag="ACHY5W_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFS4448778.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRRHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            4521..4760
                     /gene="yecH"
                     /locus_tag="ACHY5W_003992"
     CDS             4521..4760
                     /gene="yecH"
                     /locus_tag="ACHY5W_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFS4448779.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            complement(4798..5295)
                     /gene="ftnA"
                     /locus_tag="ACHY5W_003993"
     CDS             complement(4798..5295)
                     /gene="ftnA"
                     /locus_tag="ACHY5W_003993"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFS4448780.1"
                     /translation="MLKPEMIEKLNEQMNQELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(5466..5789)
                     /gene="yecR"
                     /locus_tag="ACHY5W_003994"
     CDS             complement(5466..5789)
                     /gene="yecR"
                     /locus_tag="ACHY5W_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFS4448781.1"
                     /translation="MRLLILTLSLITLAGCTVTREAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            6052..6138
                     /gene="azuC"
                     /locus_tag="ACHY5W_003995"
     CDS             6052..6138
                     /gene="azuC"
                     /locus_tag="ACHY5W_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFS4448782.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6254..6505
                     /gene="yecJ"
                     /locus_tag="ACHY5W_003996"
     CDS             6254..6505
                     /gene="yecJ"
                     /locus_tag="ACHY5W_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFS4448783.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6584..7087)
                     /gene="ftnB"
                     /locus_tag="ACHY5W_003997"
     CDS             complement(6584..7087)
                     /gene="ftnB"
                     /locus_tag="ACHY5W_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFS4448784.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT         2124 a         1585 c         1698 g         1884 t
ORIGIN      
        1 tgtatggaag gttgatgatc aggttgaaca gtttctcatg gatttagaca atgaatacca
       61 accagagcag aaagatcaaa atggttattt tctcggttct gttattatcg taaagaatgg
      121 ggaaaagcat gatgttattg acggacagca gcggttgacc actattgtca tcactatgtg
      181 tgccttacgg gatctattaa aagatagatt ggatacactt gacaaaaaag ggctggaata
      241 tctgaaaact attgaggaat ggctctctag ttatgatatt aatagtgagg aaaatctaat
      301 tagactagat cttcaatatg aagaaagtcg aggatttatt tcgcggttaa ttgaagggca
      361 aaccaatacc tttgcggaaa cagcttcagt aaaaaaaatg gctgacgcgt atgatcacat
      421 tcgtgattat cttaaatacc aattgcaaga tagcttgaat ggattaatct ccttcgctcg
      481 ctttttttta acaaaagttg agctggttgt cattgaatca gagaacttaa gtagcgcatt
      541 aaagatcttc gaaacgatca atcagcgtgg tgccagcctt aatgctatgg atcttgttaa
      601 aaatttgatt ttcagtcaga taaaagaaga acagttttcg gcaataaaga caacatggaa
      661 atccatcact aataaccttg agtcttgcgg cgaaagtgat aacccgcttc gttttttacg
      721 ttactttctg atggcacgat atcatcacgg gattttgcgt gaagatgata tatataaatg
      781 gatcatttca gctgaggggg aactgtcgtt gcagtataag acacgcccta tggtcctcgt
      841 caaagaattg gaaaaattat cagaacgata cgctaacctg gtcaatgcaa caaatgaagc
      901 agcctactgg cgtgaaaata tccgttttcc aaacgtaagt aacattggat atattaataa
      961 atatcgctca cgtcagcatt tggttttact aatggcattg gatattagtt gttcagatga
     1021 tgtaatggat ttcttaggtg ctcaacttga gagcttttta ttctttacaa atacaatggc
     1081 tattcaatcg aagacatatg agcaacgctt tactcaatgg gctgtaaagc ttcgggatgt
     1141 gaaaacgaaa gcagaggttg caaatgttgt ttcagcaaca cttatacctt ttgttgtgga
     1201 gcgtttagat gaatttaaag aacgattctc aattattaat gactataatt ttacgcctca
     1261 gtatcgccaa cgttatgtat tgggtagatt agagaatcat atccgccatc tttgcggttt
     1321 tccggaaaag agccagcagg ctattcagca gatgcaaatt gagcatattc ttcctcagac
     1381 ggtacgaaac gcccctgaaa acggggagtt ctctgatgta aatcgaacaa gttatctcta
     1441 caaacttggt aatgtaacat tattagaggg tacaattaac cagtctgtca ataactgcaa
     1501 tgatttgagt gaagactggt ttttgaaaaa acaaacagaa tatttaaact ctgaaatcat
     1561 catgactcga ttactccact cccagtactc aattggtgat aacactgcat taaatcgctt
     1621 taagctgaaa tatggatatg aatatcaaga ctggtctgcg gaaagcatca catcaaggca
     1681 acgcctgctg atggatcttg ccattgactg ctggcgattt aatgatcaga gaatagatgc
     1741 ttatatagta aataacctgg gcactggacc cggaaacgaa tttacgctgt aaaaatatcc
     1801 ttcatcgcaa agtaccccct ggaacgttat gtttaccagg ggctgggtat tcaacctcta
     1861 aaggtagcgc tgattatgaa tggatcacat tcagagagcg gttcacagaa cagatattac
     1921 taactgacag ttgtaccgaa tactgggggc aggtcaagcg ctttagcttg tgtggcaaca
     1981 acctccttta caggaccaag tagacgatta ccctgtgctg tttctgtgct caacgcaatc
     2041 tacggctatc cattttctgc cagtggttgc aaagacgacc attcaacgtc agcaaagttt
     2101 cgcaatgcac tgctatgtaa ctcattgatc ttgcgtagaa tttaaaatcc ctcggcgttc
     2161 gcgctgtgcg ggttcaagtc ccgctccggg taccatggga aagataagaa taaaatcaaa
     2221 gcaataagca gtgtcgtgaa accaccttcg ggtggttttt ttgtgcctgc aacttgtcgt
     2281 tacaccctcc ttgattttta atcaccggca aagccactca aaaagtatct ctgatacgga
     2341 ccggcatgta agataggtgc tggcgagttg agatccacaa ggaaaagcgt atgaaaacgg
     2401 gaccgttaaa cgaaagtgag ttggaatggc tggacgatat tctgaccaaa tacaacactg
     2461 accacgccat ccttgatgtg gcggagctgg acggtttatt gacggcggtg ttgagttctc
     2521 cgcaagagat tgaaccggca cagtggctgg ttgccgtgtg gggtggggct gactatgtgc
     2581 cgcgctgggc gtcagagaaa gagatgacgc gctttatgaa cctggctttt caacatatgg
     2641 ccgataccgc agagcgtctg aacgaattcc cggagcagtt tgagccgtta ttcggcttgc
     2701 gagaagttga tggcagcgag ctgacgattg ttgaggagtg gtgctttggc tatatgcggg
     2761 gcgtggcact ttctgactgg tcaacattgc ccgattcgtt aaaaccagcg ctggaggcga
     2821 ttgcgctgca cggtactgag gaaaacttcg agcgggtaga gaagatgtcg ccagaagcgt
     2881 ttgaagagag cgtagatgcc attcgactgg cggcgcttga tctacatgcg tactggatgg
     2941 cgcatccgca ggaaaaagct gttcagcaac cgatcaaagc agaagagaaa ccggggcgta
     3001 acgatccttg cccgtgcggt agtggtaaga aatttaagca gtgctgcctg cattaagaag
     3061 tcaaattggg tgtcagcgtt aattctggca cccaataagg aatttgaggc tatctgatca
     3121 ccccacttct ggtaacaacc ctgccgcaat caaaaattgc acgccaatca cagcaatacc
     3181 acagagaaac accaccacca gcgccggacg accacctttg acccggtaac ccgcttgagg
     3241 attgtgcctt ctgctttgcc aggtcaacag cgaagggata atcaatgcca gtaccgccag
     3301 cgccacaccg gcgtaaccca gcgccatcac aaatcctcgt ggataaaaca gtgcaaacgc
     3361 caacggcggc agaaaggtaa ttgctccagt ttgcaatcgt ccaccaacgg tatttgaacg
     3421 ctgaaacaaa tcagccagat aatcaaataa gcctaacgca acgccgagaa atgacgtggc
     3481 gagggctaaa tcagcaaata aatgcactgc cagctcaaca tgcggagagg ccaccatttc
     3541 gcgtaacgcc tgtaacagcc cgtttaatcc ggcatgatta gccagcaatc ccataaaggt
     3601 tgttgaatca atgctgccaa gcgtcgccac ctgccagaaa atatatgcca ccagggggat
     3661 cgcactaccg gttataaaca cccagcgtag cttacgaatg ttgccatcca tatagctgac
     3721 aatactcggc acgctaccgt gaaaaccaaa cgacgtaaaa atcaccggga ttgcagacag
     3781 tgccagcccc tgttgcaacg gcagggttaa aagattcact ttgtgaatat gcggtagcag
     3841 caataccaac atcaccacca aaaaaataat tttggcgctg aacagaaaac ggttaaataa
     3901 atcgaccagc gatgttccaa cacaaaccac gccaccggca acaaaagtga acaacagcac
     3961 gccagcggtt gccgacattg aaatacctgt ccagtcgctg atgctggagg ccaacaattc
     4021 accggcaccg ctgatgtatg ccgcagtcag agcatacatt aagaacatca tactgaagcc
     4081 cgtcagccat tgaccgtagc gtcccagata gcgttttgcc agcgtgccca gaccggtatc
     4141 tgccggaaca tgctggtaca cctccagcag taatagcgcc gtgtagcaca tcaacgccca
     4201 aagcccaatc aacaagatta acgtaacgct aaaaccaaca ccggccgcag ccagcggcat
     4261 tgccagcatg cctgcgccaa ttgtggttcc cgccacgata aaaacacttc ccagggttct
     4321 gtttttcacg ctttcttctg tcctgacgat ctttatgagt gatatctgcg gcgcaggtta
     4381 aggcataacg gctgcttcgt caaaccgacg ttacatatgg tgtaaatata aatgtacaat
     4441 aaaaagcatt cagtcctgcg ctggcgcaaa gtcttcataa gcggtaatcg ctacgctagg
     4501 cttttttttg aggaggaacg atggactcta tacacggtca tgaagtgtta aatatgatga
     4561 ttgaatcagg cgagcaatat acgcatgcca gtctggaagc tgcgattaaa gcgcgttttg
     4621 gtgaacaggc acgttttcac acctgctcgg cagaagggat gacagcggga gagctggtgg
     4681 cgtttctggc agcaaaaggc aaatttattc cttctgaaga cggtttttcg accgatcaga
     4741 gtaagatttg ccgtcactga tttgaaacgg cggcagaaaa cctgccgccg gagagcatta
     4801 gttttgtgtg tcgagggtag agagttcttt gtcgataaaa tacagacctt cgccgctttt
     4861 gcctgccagg cttaatttat caataatcga tttgaacagt ttctcttctt catgctgctc
     4921 agaaacatac cattgcagga aattaaatgt tgggtagtcc tgattggtca ttgcagcatg
     4981 agccagttcg ttaattttct gcgtgattaa ttgttcgtgt ttataggttt cctggaataa
     5041 ttcatcaagt gaagaatatt cagcaaacgg agattcaacg gtattaatac gcggtaagtt
     5101 tccggtatca gtcaggtaat caaacagacg ctgcatatgc gtcatctctt cctgggcgtg
     5161 gcggcgcagg aacgcggcag caccttcgaa ggtatgatag ctgcaccagg cgctcatttg
     5221 ctgataaagc agtgaagagt acagttcctg gttcatctgc tcattaagtt tttcaatcat
     5281 ttctggtttc agcatgatag tgctccacaa aggttatatt tgttgctgtg gcgtcactat
     5341 aatttgttat tttattattt gcaaaaggta aaatataaac ttcaattatt aaaaatacga
     5401 atgggaataa aacgcattac cgctatttct ttaatcagcg aagatcccct cgctgattaa
     5461 aatatttaga gaaagacagg gaaggcggaa tgatggcact gatatgacaa agtgaattcc
     5521 gtattcaaac ataaggaacc cgcaaaaagg ctacagttgc ctactggctg accaaagggg
     5581 atcgcgtggg tatagccttc ctgctggcat gcgcgatcgg cgatcccgcg actgacataa
     5641 cgatcggtat gggcatgctg caaaaaagcc tggtcataaa ccaaccgtac aatacctgtt
     5701 gccgcgtcaa cttcgctcac atgggcctcg cgagtaaccg tacaccccgc cagcgttatt
     5761 aatgaaagag tcagaatcag aagtctcatg gtccagccag aagagtaaga gaaagcacta
     5821 ttttacgcgt tacagcgctg cgacaatggc cggaaaagac gcctggcagg cgctatttct
     5881 tgccatctgt aggtgtcaga aatagggggg ctgttttttg gttatcattt ttataacatt
     5941 aaaggggtat ttatgtgatt tgtatcacat aaagattgat ttttgtgatg gttgtcatat
     6001 tataagcgca aagacaataa cacctgtata acaaatggtc ggagtgccgc gatgaaactg
     6061 cgcaaaatcc tgaaaagtat gttcaataac tattgcaaga cgttcaaaga cgtaccgcca
     6121 ggcaatatgt tccgataaca aaaaaacctg ctccggcagg tttttttgtg tcctggtgac
     6181 ggtgggtgtg actatcgctg aagatgtgaa gccgctacta taacggcttt caaaatgcta
     6241 tcaggagttt acgatgtccc agccgctgaa tgccgatcag gaactggttt ctgatgtggt
     6301 tgcttgccag ttggttatca aacaaattct cgacgttctg gatgtaatcg cgcccgttga
     6361 agttcgcgaa aaaatgtcca gccagctgaa gaatatcgac tttactaacc atcccgccgc
     6421 cgcagacccg gtcaccatgc gggcaatcca aaaagccatc gcgctgattg aactcaagtt
     6481 taccccacag ggcgaatccc attaaaaaac cgctgcttca aaaagatgag gcagcggttt
     6541 ctcaaaaact tcatcggcgc aatgcattag cgccgatgta tgatcaatgc agctgatgcg
     6601 acacgacatt cagaacatgt tggtcggtct gcacagggca catacccgca agtttcgcac
     6661 tgcgcacttc atcaagaatg gtttgcagca acagaccatc atgctgctgt tctttttcca
     6721 gatcgcgcag gaaattgacg gttgaatcat cattcagttc tttcgcttca tcggctaact
     6781 gtgccaacgt actagaacgt tgctcgtatt cttccatcgt tttttggaac agttcttcca
     6841 gagagttcag tttttcaccg ggaacatcaa tggctttaac gatgggggta gcgccgacac
     6901 tcttcataaa gttaaacatg cgcatcattt gggtcacatt actctgtgcc tgggcgcgaa
     6961 ggaaagtggc ggtgccgttc agactctgtt cagaacacca gttactcagg tgaaggtaga
     7021 gattggatgc gtaaaactcg cggttcattt gagagttgag tttgagaagc attccagcgg
     7081 ttgccatacc taatatcctt atatccagaa gtggagaggt gcaagataaa tcaaaacacc
     7141 ataattactg tttgcagaat aattcctttc tgccggtccg aaatgtgatc gtggagtcaa
     7201 ttctgacgat gatctgaaat aagaataacg tctgaacggg tataagaaaa tgcttaaatc
     7261 atcaagttta tatattttta catttcattg a
//
LOCUS       DAZUGK010000211         7251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZUGK010000211 DAZUGK010000000
VERSION     DAZUGK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.212"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(117..401)
                     /gene="ppnP"
                     /locus_tag="ACHY5W_003998"
     CDS             complement(117..401)
                     /gene="ppnP"
                     /locus_tag="ACHY5W_003998"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFS4448785.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(473..1150)
                     /locus_tag="ACHY5W_003999"
     CDS             complement(473..1150)
                     /locus_tag="ACHY5W_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFS4448786.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1408..1599)
                     /gene="yaiA"
                     /locus_tag="ACHY5W_004000"
     CDS             complement(1408..1599)
                     /gene="yaiA"
                     /locus_tag="ACHY5W_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFS4448787.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1649..2173)
                     /gene="aroL"
                     /locus_tag="ACHY5W_004001"
     CDS             complement(1649..2173)
                     /gene="aroL"
                     /locus_tag="ACHY5W_004001"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFS4448788.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2356..2814)
                     /locus_tag="ACHY5W_004002"
     CDS             complement(2356..2814)
                     /locus_tag="ACHY5W_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4448789.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2934..3743
                     /gene="proC"
                     /locus_tag="ACHY5W_004003"
     CDS             2934..3743
                     /gene="proC"
                     /locus_tag="ACHY5W_004003"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4448790.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3760..4875)
                     /gene="adrA"
                     /locus_tag="ACHY5W_004004"
                     /gene_synonym="dgcC"
     CDS             complement(3760..4875)
                     /gene="adrA"
                     /locus_tag="ACHY5W_004004"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFS4448791.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4977..5297)
                     /gene="psiF"
                     /locus_tag="ACHY5W_004005"
     CDS             complement(4977..5297)
                     /gene="psiF"
                     /locus_tag="ACHY5W_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFS4448792.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5416..6831)
                     /gene="phoA"
                     /locus_tag="ACHY5W_004006"
     CDS             complement(5416..6831)
                     /gene="phoA"
                     /locus_tag="ACHY5W_004006"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFS4448793.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6932..7192)
                     /gene="iraP"
                     /locus_tag="ACHY5W_004007"
     CDS             complement(6932..7192)
                     /gene="iraP"
                     /locus_tag="ACHY5W_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFS4448794.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1774 a         1904 c         1731 g         1842 t
ORIGIN      
        1 tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaaggaaat attattatgg
       61 gttaaagaga ggatatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac
      121 agatagcggc acagataaga ggtgggttcg gcaacttgca gatgaaactc actgtgaccg
      181 ggaacattaa acaccgaacc ggcttcatac acttgccagt cggtcgcgtc aggcagtaac
      241 acattcagcg cgccactgat taccgtcatt tcttccggct cagcggtgct gaaggtgtat
      301 tcgccttcaa ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatc
      361 gatttcactt tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtctggta
      421 gttgaacatc tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacacca
      481 gtaattccgc agctagccgt gcaatcaata cgttagacag caagacagga acatcgagct
      541 gtttttgcag taaatcgcga tgacgttggt gaaatcctaa acaatccagc atgatgacat
      601 cagccccttt tgccagtaat tctttcccgg catcaatgat tttttgttct gaatcatgaa
      661 tggggttacc caatgaaaat accggcgatt tctgcaaaat ttgccatttt tgcgcctgaa
      721 cgggcagcat ctcctcaacc ggaacgataa cccccacctg atgatcttca acaatagagg
      781 aaaccagtgg aggcaatatt cgcgacggct caagaaagat cgtattacgc gcagtcatac
      841 tactaatgtt tgctgtactc attaataaaa tgacgtcata accctgatta tcgagcactt
      901 caaccacacc ttgcaggtct cgctccactt tacgacgcga aacatgggcc agctggttgt
      961 catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt
     1021 cttcacgact taacttcccc agcaggctat gatgggaaat attatcttcg tcaatgtatt
     1081 ccgtcaggag cggcaaaact tcctgcatag gtacaatgcc gatggtcagg atcgccaacg
     1141 acgcactcat tgttcatcca ccttactttt atttcgtcgt taattaccgg gcaagtgtta
     1201 agcaccactg ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacgtgc
     1261 acggcataat gtaaagattc caaatgattc cagtaatgga ttgttattgt ttaatattct
     1321 aattattaga aaaacatgaa ttatgaaaaa tgtgacgcag atcccttgta gtggcactca
     1381 gaatcccttc cgagcagtct gatgcggtca ctctttgtca gggtcctcat agcgtttttt
     1441 cgcatccatc gcttcctgag cggtcggatg ttcactgatc aacgagtctg gtttaggatg
     1501 atcggctctg agttggtacc aggttaccgt ctgtcctggt tttccttttt caatcgtcac
     1561 tatatatgct tcacgaggat aaggcggttt cgttggcatc gttcttcctt atttcacggg
     1621 atgaacgtta agtataggcg ctcgaaaatc aacaattgat cgtctgtgcc agggcgctgc
     1681 gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc gatgataata tgcgcaactt
     1741 cgcgatatag cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc agcggttttc
     1801 ccgttaaggt tggccgtaaa tcttcttccg gcgcagcttg cagtcggtta accaggactg
     1861 atactggcgc acacaaataa accacgatcc cgttattttg catgaagtga cgattaaatt
     1921 ccgtcagaat aatgccgccg cctgtagcga taacggtgga tggcgcagtt accgcttcca
     1981 gcgccgccgt ttctctggcg cgaaatcccg cccactcttc cctttcgacg atctccgcga
     2041 ccgtcatatt gagctgtgat tgcaaccact gatcggtatc gacaaaccga cggttaagcg
     2101 aatcggcaag ggccattccg accgttgttt taccacagcc ccgaggcccg atcagaaaaa
     2161 gaggttgtgt catcgtgggt tttccccaat aggtcgcaat gcggcgaaag ccggtgtcat
     2221 gagaatagcg atcataccat caaactagta caatttcgat tgtaaagaaa aaattccact
     2281 taaagtgaaa atctcaatac atcccttact ataccaataa atattcaaga atgaagtgta
     2341 aataataaat tacatttagc cacgactacg ttgcacttcc agccaccact tctccagctc
     2401 cgcggcaaag gcctggcggt cacgttgtga aaggctatct ggtccgccgg tctggatccc
     2461 actggcacgt aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa tggtcgctgg
     2521 cgtgtaacgt tcaccgcgcg gattaagcgc cgcagcgcct ttctcgatgg cttcagcagc
     2581 caaaggtata tctgcggtga tcaccaaatc gcccgcttca cactgccgga caatttcgtt
     2641 atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg atggcggcac
     2701 gcgtaaactc tggtttgcta ccagtaccag cggcatctgc atacgttccg ccgcgcgata
     2761 caaaatctct ttaattacat tgggacacgc gtcggcatcc acccaaattg tcataaagtc
     2821 atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc cttccggcat agttctgttt
     2881 atgcttctgc cagcgattat caaaacaatg aatttcacgg caggagtgag gcaatggaaa
     2941 agaaaatcgg ttttattggc tgcggcaata tgggaaaagc cattctcggc ggtctgattg
     3001 ccagcggtca ggtgcttcca gggcaaatct gggtatacac cccctccccg gataaagtcg
     3061 ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa gtggcgcaaa
     3121 tcgctgacat catttttgcc gccgttaaac ctggcatcat gattaaagtg cttagcgaaa
     3181 tcacctccag cctgaataaa gactctctgg tcgtttctat tgctgcaggt atcacactcg
     3241 accagcttgc ccgcgcgctg ggccatgacc ggaaaattat ccgcgccatg ccgaacactc
     3301 ccgcgctggt taatgccggg atgacctccg taacgccaaa cgcgctggta accccagaag
     3361 ataccgctga tgtgctgaat attttccgct gctttggcga agcggaagtg attgctgagc
     3421 cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc agcctacgta tttatgttta
     3481 tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc acgcgctcag gcgtataaat
     3541 ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct ggaaacggga gaacatccgg
     3601 gagcactgaa agatatggtc tgctcaccgg gaggcaccac cattgaagcg gtacgcgtac
     3661 tggaagagaa aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt atggaaaaat
     3721 cagaaaaact cagcaaatcc tgatgacttt tgccggacgt caggccgcca cttcggtgcg
     3781 gttacgtccg gctttctttg ctttgtaaag cgccaaatct gccgatttca accactcacg
     3841 atagtgactc atttgtgggt tcagcggtgc aacccccaca ctaatccgta aagttacctg
     3901 tggcgtattc ggcaagcgta acgtgtttag cccttcatgc acccgtaaca tggcggtaat
     3961 ggcgctctca gctggcgtac cggacatgat taccgcaaac tcatcgccgc caaaccgacc
     4021 aatcacatcg ctaccgcgca gggtaatttg taactgtcgg gtaagcgcca caatcgcttc
     4081 atcgcccaca tcatggcccc aggtatcgtt gatgctcttg aaatggtcga tatcgataat
     4141 cagtaacgtt gcatcgcgat tatgccgccg acagttatca aattcattgc gtaacatagt
     4201 ttcccaatga cgtcggttat acacgcccgt catgccgtcg cgggtactca tgacctgcaa
     4261 cctgcgttta tgttccgcca gtttggttgc cgtctggtag ctgacccagc caaacagcag
     4321 aggataaatg acaataatgg gaagggagag ccaccattcc agcggcgcgc tattgaacga
     4381 cacggtaatg cccgtcagct cgagggtgac aaggcaggaa accaccatca acaccagacc
     4441 cgcgacaaac agacgggggc cgcctgcccc catcaaattc agacacataa tcatcaacat
     4501 cgcggtggaa ggcagcacgt ttacgcccat tacgcctacc cacattcccg ctaatactgc
     4561 atcggttttt aagttgtaaa tttcccggct aagcggatcg acggccctgc tcgctatctg
     4621 ccaggctaaa tgcggccaga cgaacgccca gccgaccaac accagccacc accagcccgg
     4681 cggcgggtgt gaaaccagcg ttgaagcaat cggtaagaac atgccagcca ggccaaccgc
     4741 acggggtagt ctgacgcgac gggcgaagcg cagcccggac cgctgatgtt cgttttgagg
     4801 agttaaagga ggttcctccc cgtgcgccgc cgcttttttg aaaaagtttt catcattcat
     4861 tatttttggg aacattcaga aacaattttc ccaaattata gagacggggc taaaggcaga
     4921 agtatgaaat ttcgggtgag ccattttagt agctcacccg ttagcactat caggtatcag
     4981 gcggctttct tgaggcaggc gctcataaac ttattacgat catcaccttt cagtgattgt
     5041 tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa acttttttca
     5101 ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct
     5161 ttcaacgcct gtgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgtggggtt
     5221 aaggttctct cggcagcacc gacggtggtt aaaaaaacca gaccgaaaag caaggtaacc
     5281 agtaatgtaa ttttcatagc accatccctc ttcatgtttt aaccatgagc gtatgcgccc
     5341 gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca
     5401 ctgccgggcg cggttttatt tcagccccag ggcggctttc atggtgtaga agagatcagt
     5461 ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa tacgcaactg
     5521 actgcctgta tgttcttgtg aatcctcttc ggagttcccg taactcatca ccatcactgc
     5581 gccatctttg gtatttagcg cctgggtgag gcccggagct ttggtatccg gcgcaacaat
     5641 ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct cctttttagc
     5701 gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca
     5761 aggattcgca gcatgatcct gtttatcgat tgacgcacct tcaacttgca ggaaaaagcc
     5821 tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgcgcca gggttggtac
     5881 actgtcatta cgttgcggat taggcgtaca ggtgactgcg ggcttatcaa tattaccgtg
     5941 atacgttgct ttcggtccta gccagcgcac tggcatattg ccgtcagcaa acaggccaag
     6001 caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat cgctcaccaa
     6061 ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt caccagcggt
     6121 tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac gagcgttaag
     6181 cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc
     6241 actggtcgcg ctcggaccgt agcatttgcg cgaggtcaca tgtgccacca gcgcagcggg
     6301 cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc
     6361 catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg cgccgttata
     6421 ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga cgtagtccgg
     6481 tttgccggtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaaggcatc
     6541 gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag taatttccga
     6601 atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat cgctaagaga
     6661 atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg gtgcagtaat
     6721 atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg cttttgtcac
     6781 aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca ctttattttc
     6841 tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg gccgtgacaa
     6901 ctttatgaca gcagttgaaa agattaactt tttactgacg aggatgcttc aataacttct
     6961 ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat gctggcatcg ggctttacct
     7021 cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc gccatattgc
     7081 gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt tctttcgact
     7141 cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc atgtctgtat
     7201 ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata g
//
LOCUS       DAZUGK010000212         7227 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZUGK010000212 DAZUGK010000000
VERSION     DAZUGK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.213"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            83..1416
                     /gene="adeQ"
                     /locus_tag="ACHY5W_004008"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             83..1416
                     /gene="adeQ"
                     /locus_tag="ACHY5W_004008"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1469..1921
                     /gene="yicN"
                     /locus_tag="ACHY5W_004009"
     CDS             1469..1921
                     /gene="yicN"
                     /locus_tag="ACHY5W_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFS4448795.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2037..2360)
                     /locus_tag="ACHY5W_004010"
     CDS             complement(2037..2360)
                     /locus_tag="ACHY5W_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448796.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2344..2697)
                     /locus_tag="ACHY5W_004011"
     CDS             complement(2344..2697)
                     /locus_tag="ACHY5W_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4448797.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2931..4121
                     /gene="nepI"
                     /locus_tag="ACHY5W_004012"
     CDS             2931..4121
                     /gene="nepI"
                     /locus_tag="ACHY5W_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFS4448798.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4162..4455)
                     /gene="yicS"
                     /locus_tag="ACHY5W_004013"
     CDS             complement(4162..4455)
                     /gene="yicS"
                     /locus_tag="ACHY5W_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFS4448799.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4677..5495
                     /gene="nlpA"
                     /locus_tag="ACHY5W_004014"
     CDS             4677..5495
                     /gene="nlpA"
                     /locus_tag="ACHY5W_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFS4448800.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5499..6422)
                     /gene="yicL"
                     /locus_tag="ACHY5W_004015"
     CDS             complement(5499..6422)
                     /gene="yicL"
                     /locus_tag="ACHY5W_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFS4448801.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6533..6706)
                     /locus_tag="ACHY5W_004016"
                     /pseudo
     CDS             complement(6533..6706)
                     /locus_tag="ACHY5W_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6886..6991
                     /locus_tag="ACHY5W_004017"
                     /pseudo
     CDS             6886..6991
                     /locus_tag="ACHY5W_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            7002..7067
                     /locus_tag="ACHY5W_004018"
                     /pseudo
     CDS             7002..7067
                     /locus_tag="ACHY5W_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1768 a         1607 c         1806 g         2046 t
ORIGIN      
        1 aaatgtgata attatcactg aaccgttgtt gtcatttgtt ttacaaaagc aagggattaa
       61 atctcctcca atggacaaaa aaatgaataa tgacaatacc gattacgtga gtaatgaatc
      121 agggacgctt tcgcgattat ttaaactacc tcagcatggg accaccgtcc gcacagaatt
      181 gattgcgggg atgaccactt ttttaaccat ggtgtacatc gtttttgtga acccgcaaat
      241 cctcggcgcg gcacaaatgg acccgaaagt ggtgtttgtt accacctgtt tgattgccgg
      301 tatcggcagt attgcgatgg ggatatttgc taacttaccc gtggcgctgg ctccggcaat
      361 ggggctgaac gccttctttg cgttcgtggt cgtgggggcg atgggtatct cctggcagac
      421 cgggatgggc gcgatattct ggggcgcaat tgggctattt ttgctcacgc tatttcgtat
      481 ccggtactgg atgatctcca acattccatt aagtttacgt attggtatca ccagcggaat
      541 cggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
      601 cactctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      661 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgcttg tttctattgt
      721 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      781 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      841 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      901 aattggtgta actgataaag ctggcttaat agatagtaac ggtaaattcc ccaatatgaa
      961 taaggcgcta tatgttgata gcgtagttcg gtggcgggcg cgtttatcgg cacctcgtct
     1021 gttaccgcct atattgaaag tacttctggt gtggcagtcg gtggtcgcac ggggctgact
     1081 gcggttgtgg tcggcgttat gttcctgttg gttatgttct tctcaccgct ggtggcgatg
     1141 gttcctcctt acgcaaccgc cggagcgtta atctttgttg gcgtgttgat gacctcaagt
     1201 ctggcgcgcg ttaactggga tgattttacc gaatcggtgc ctgcgtttgt taccacggtg
     1261 atgatgccct ttactttctc gatcaccgaa gggattgcac ttggctttat gtcgtactgc
     1321 atcatgaaag tgtgcaccgg gcgctggcgc gatctgaacc tgtgtgtggt ggtggtcgca
     1381 gctctgtttg cactgaagat tattctggtg gattagtaac tggcgagggt ttccagatat
     1441 catgagttct gattacgcag gagaactcat gatctggata atgctcgcca cgctggcggt
     1501 agtgtttgtg gttggttttc gggtgctgac atccggggcc agaaaagcga ttcgccgtct
     1561 cagcgatcgg ctgaatatcg atgtcgtacc cgtggagtcg atggtcgatc aaatgggaaa
     1621 gtcagccggt gacgaatttt tacgttattt gcatcgtccg gatgagtcgc acctgcaaaa
     1681 cgccgcgcag gtgttgctca tctggcaaat tgtcattgtc gatggtagcg aacagaacct
     1741 gctgcaatgg catcggattt tacaaaaagc ccgccttgcc gctcccatta ctgatgctca
     1801 ggtcaggctg gcgctgggtt ttctgcgcga aaccgaacct gaaatgcagg atattaatgc
     1861 ttttcagatg cgctataacg cgttctttca gcctgccgag ggcgttcact ggctgcatta
     1921 aaaccgcttc gtcttatcag cctacagacc gcgcctgacc tgtagggtgg ataaggcgtt
     1981 cacgccgcat ccggtatcgg aactgacagt aggtgaacgc attatctacg ttctatttac
     2041 agcagcacct ccaacccgtg tttttgcaca atgctgagca gttttaatga aatgccattg
     2101 gggcgtttag cgccactttc ccatttttgt acagttgaaa cgctggtatt gagatagtga
     2161 gcgaagacgg gctgactcac ttttaatttt tcccgcagtg cttttatctc aacaggttgt
     2221 aagtcttgca cgctggtcag gcaggctttg tcaaaagtac gcatcgtttc agcagaaata
     2281 gcattgacgc tggacaatcc agccgctgcg ctgtgaatag cctcaaatgc aggactttta
     2341 aatttagttt ttgcagtcat agcagatctc tgtcagttct ttagtgttaa tcattgctgt
     2401 tagttgggct ttcgtcagga aggcataatg ctttgcaatt tcacgaaacc cagccagttc
     2461 ctgactattg atgttcgaca tatcctgttt tgcatataaa aacgtgtaaa accaattgct
     2521 tacccctttc gccagaataa ttgcacgctc acgattttgg tgaaggcgct ttttaaaaac
     2581 gccaccgcca agtgcatcag ctttaccttg ttctgtttct aaaatggccc ggcacaactc
     2641 ttcatctgtt attgcatgag acctggcttc ccttgcaaac catttggttt taaacacgcg
     2701 tatatcgttg aggtcctgtt attaaagtat atcaccaagt gcaatacttt atcccttgtt
     2761 cccaacacaa tcgcgaaaat acatatcacc ttgccctatg atttttattt cttcgagcag
     2821 cctcgagctt ttcccacaac accgtaaaag ttgcaaaaac gttaaatgcg tcacacattt
     2881 caatgttaaa gtggtcggct tttcccctga aacatgccac gggtaacacc atgagtgaat
     2941 ttattgccga aaaccgcggc gcggatgcca tcacccgacc gaactggtca gccgttttct
     3001 cggtggcgtt ttgcgtcgcc tgtctgatta tcgttgagtt tttgcctgtc agtttgttga
     3061 cgccaatggc ccaggattta ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga
     3121 ccgcctttgt ggcaatgttt gccagtttgt ttattaccca gacaattcag gccactgacc
     3181 gccgctacgt tgttattttg tttgccgttt tgctgacgct ctcctgcttg ctggtttcct
     3241 ttgctaactc attcagtttg cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg
     3301 ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt gccgccgcgt acggtgccga
     3361 aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg
     3421 gctgtttttt aggcgagctt atcggttggc gcaatgtctt taatgcggcg gcggcgatgg
     3481 gtgtgctgtg tattttctgg attatcaaat cactgccttc gctgccaggt gaaccttcgc
     3541 atcagaaaca aaatactttc cgcttattac aacgtccggg agtgatggcc gggatgatcg
     3601 ccatctttat gtcctttgcc ggacagtttg cttttttcac ttatattcgc ccagtgtata
     3661 tgactctggc gggattcggt gttgatggct taaccctggt gctgctgagt tttggtatcg
     3721 ccagctttgt cgggacatcg ctttcgtcgt ttattctcaa acgttcggtg aaactggcct
     3781 tagcgggcgc accgtttgtg ctcgccctga gcgcgctggt actgacgctg tggggaagcg
     3841 ataaaattgt tgctactggt gtggcgatta tctgggggct gacctttgct ttaattcccg
     3901 ttggttggtc aacgtggatc actcgctcat tggccgatca ggcagaaaag gctggatcta
     3961 ttcaggttgc cgttattcag cttgccaata cctgcggtgc ggcaatcggc ggttatgcgt
     4021 tggataacat tggtctgact tcgccgctga tgttgtccgg cacattgatg ttgctgactg
     4081 cattgttggt gactgcaaag gtgaaaatga agaaatcctg atatcaggta aagaagcgtt
     4141 ggtcagtggt tgttgccgtt cttacatatc cgggcattct accttaccca acgcttccca
     4201 ataattgctc tgattattac gttccatcgc cacgatggcc tcgcgtatat gttgtttatt
     4261 gttttcatct gacaacatta acttttccca cgcttcatct gataatgacg cctgtggcgt
     4321 gcaaatttta cgtaaatctt gcaataccgt ggttttctct tttgccgact gtagagaact
     4381 gaatggcgac tcggcctgag aaagggctgg aaaaatcagg aatacggtaa tcatgagtag
     4441 catcgttggc ttcatagcgg caaccttctg caaatgtgca tcgaatgaag cataagtgta
     4501 gtttgctttt gccgctctca tcatgctgta cgaaaggtgt ttgcgtggtc agtaagaagt
     4561 gccagacttt atattccact tttattcctt tttattctta tcgatagcgt ttcgtttttt
     4621 aaactgcagc gaccttaccg ctatagtccg gtaatcatta ataaaaggat aaaaaaatga
     4681 aactgacaac acatcatcta cgggcggggg ccgcattatt actggcagga gttctgctgg
     4741 caggttgcga ccagagcagc agcgatgaaa agcatattaa agttggcgtt attaatggcg
     4801 cagaacaaga tgtcgcagaa gtcgctaaaa aggtagcaaa agagaaatat ggcctcgatg
     4861 ttgaactggt aggatttagc ggttcgttgc taccgaacga tgcgacaaat aatggcgagc
     4921 ttgatgcgaa cgtcttccag catcgaccct ttcttgaaca agataatcag acgcatggtt
     4981 ataaactggt agcggtggga aatacgtttg tcttccctat ggcgggctat tccaaaaaaa
     5041 tcaaaaccgt ggcgcaaata aaagaaggtg cgacagtggc gatccccaac gacccgacta
     5101 accttggtcg cgcgctttta ctgctgcaaa aagagaagct aattactctg aaagagggaa
     5161 aagggttatt acctaccgcg ttggatataa ccgataatcc acgccatttg cagataatgg
     5221 agctggaagg ggcacagttg ccacgtgtac tggacgaccc gaaagtggat gtagcgatta
     5281 tcagcaccac ttacatccag caaacaggac tttctccggt taacgacagc gtatttattg
     5341 aagataaaaa ttcgccgtat gtgaatattc tggtggcacg ggaagataat aagaatgcag
     5401 aaaacgtgaa ggaatttctg caatcttatc aatcacccga agtcgctaaa gcggcagaaa
     5461 ccatctttaa cggtggtgcg gtgcctggct ggtaattctc actcatgccg cgccggacga
     5521 tttatttttc tggcgcggcg gcgggaatcc attgaaatca aaatcaccga tgacagaatc
     5581 agcagcgttc ccagccagtc cggcaatgtg aaggtgatcc ccagcaacag caaagagagc
     5641 agcgcgctac ttaatggttc agcacagctt aaaatgctgg cttttggacc gccaattaat
     5701 tgtgctcctt tcaggtacag actaaatgtc agggacgtac caatgaccac caaataaaaa
     5761 aacgccagaa tcaaacttcc gttaacgaca aagtttgtcc cttgtccggc ataaaagggc
     5821 agcaggatca gaccgccaat gagcatactc cagccgacga ccggtaacgt gccatagcgg
     5881 gcaatcagcg ttgagggata ggtggtatag aatgcagcag taaatgccga ggcaatgccc
     5941 cagaataacg cggcaggaga gatcgataat gacgtcggat taccgtgtgt caccaataaa
     6001 aaagtcccga tcagcgatgt caaaatagcg cagaaaacca gaatgcccgg gcgggattta
     6061 cgcaccagtg agaaccaggc gacgataata gtcggtgaga ggaattgcag caccgttgcc
     6121 gtggctgcgt tcgatttttc gatggttagc aaaaaagtga gctgtacggt tagcgcgcca
     6181 accacggaaa aaatcagtag gctaatggca tctttgtgat tgttaataat agaaaagatt
     6241 ttatcaccat gaacaaatga tagcgtcagc agaatcaaac cggcgaatat caaacgcgtc
     6301 atagtcaaaa actgcgacga catctggctt tgctccatga tgtattgcgc gcagacccct
     6361 gaacttcccc acaacacggc ggcaatcaga acgttcagca tcccctttct ggtggaaccc
     6421 atttttccct ctgcatactg ttttgtcgtt tatgtatgca ggcatgatag caaaaatggc
     6481 gaggatggct cagtttgcgc cacgccgatt tttcttagcc cagactggtg agctaaatat
     6541 ctttaataaa cagcaagcaa atcattgcaa tacccaacat ccctatcgcc agccagaaca
     6601 acgtgtggta gctccagatt tcaaccataa tttcgtcaac agagccggcg ataatctagc
     6661 cgaagcgtga ggtatttgca tataacgttg ttgcggaacc tattcttatt tatcggagtg
     6721 ccttgccagt aatttactga cgaggatcct cataatagcg ttacccatct gttgcattga
     6781 gactttacag gccgttattc taaatgacca aaattaaatc agtgcataag gggaagtatt
     6841 ctgtagtgat gtctccctga gatgcaggtt ccactggaga aaattatgag cagtttgttt
     6901 cagaatgaaa taaaggataa atttaaaact ggacttgcat atgtgcgggg gccagaaaaa
     6961 agcggatctt ccgctcaaaa tgcaggtagc gaaggtggta aaaaatgcgc cgggagaagc
     7021 agaagattgt cccgcgatgt ccgtttgaga aacacgagtt tgattaaacg cgtgagcatg
     7081 atgatattag tagctgacca gagaaattat atccgtcgta agcaataaaa atcagaagtg
     7141 atgttatgga tatttttaac ctgaataact ccgtaagtac ggggttattc aggtttttgc
     7201 tagcctgtgc ttttctatac cagatgt
//
LOCUS       DAZUGK010000213         7147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZUGK010000213 DAZUGK010000000
VERSION     DAZUGK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.214"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..296
                     /locus_tag="ACHY5W_004019"
     CDS             120..296
                     /locus_tag="ACHY5W_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448802.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            293..652
                     /locus_tag="ACHY5W_004020"
     CDS             293..652
                     /locus_tag="ACHY5W_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448803.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKQRVGDWKY
                     VPTKEDCNDRPNYEIQALFTTQAVPLTPEGLIKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLKEACQAVNIGIKGE"
     gene            655..942
                     /locus_tag="ACHY5W_004021"
     CDS             655..942
                     /locus_tag="ACHY5W_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448804.1"
                     /translation="MATLQELIGLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTARVYMPSIDAPGLTSWADDWHGITLKDGVEVDED"
     gene            946..1290
                     /locus_tag="ACHY5W_004022"
     CDS             946..1290
                     /locus_tag="ACHY5W_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448805.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPSTIDHDIIRTYKMDIAVLE
                     IALASLAAEPVGKLHEYKPVGYQRLVDELTMQVKQLAWQLRKAKPDCKLPDKAMDYLE
                     RNGLTSVEDVLR"
     gene            1622..1801
                     /locus_tag="ACHY5W_004023"
     CDS             1622..1801
                     /locus_tag="ACHY5W_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448806.1"
                     /translation="MSYALKKHTRLNIPPRDKRVVAAPRPAIDENCTHREQVKNAFDF
                     GFSRYEKAMEELSKV"
     gene            1820..2305
                     /locus_tag="ACHY5W_004024"
     CDS             1820..2305
                     /locus_tag="ACHY5W_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4448807.1"
                     /translation="MAEIVEGVHYLTVDDLVEINRSLIELQTPDEPVGVLSPDNLSSS
                     QARPSMVRWYKQINDMFVLASVLIESLIQNHPFANANKRTAMMAGYVFLLLNGYELTA
                     PGDEIVEMAEGLACKTYTREDLENWLCYWSRAYDSRELCKTGATIVLYETIKLKIEQQ
                     N"
     gene            complement(2283..2480)
                     /locus_tag="ACHY5W_004025"
     CDS             complement(2283..2480)
                     /locus_tag="ACHY5W_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448808.1"
                     /translation="MPAGRGGRVIRIVKSGIVRHQFSLLTQQVPVVQAGVRIVNQNAR
                     KKQNPPKRVFIGSTFSFAVLF"
     gene            2421..2699
                     /locus_tag="ACHY5W_004026"
     CDS             2421..2699
                     /locus_tag="ACHY5W_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448809.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWFRHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2701..3750
                     /locus_tag="ACHY5W_004027"
     CDS             2701..3750
                     /locus_tag="ACHY5W_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4448810.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVICAAGGAGALSDWLLRQVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            3763..4122
                     /locus_tag="ACHY5W_004028"
     CDS             3763..4122
                     /locus_tag="ACHY5W_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4448811.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGIKITELECA"
     gene            4119..4802
                     /locus_tag="ACHY5W_004029"
     CDS             4119..4802
                     /locus_tag="ACHY5W_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4448812.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLELEEHQK
                     AWLLWCYSGNVCWEDQVTITQWVWSEFKAQSGTRKIAGKTQERLKRLIWLAVQDVKSE
                     LVGRETYEHQALAVLMGVAKSTWTTTHRPHWLAMRGSAEGLDRDALISVMRVRSRQQS
                     LNLEPYLAK"
     gene            5021..5218
                     /locus_tag="ACHY5W_004030"
     CDS             5021..5218
                     /locus_tag="ACHY5W_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4448813.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            5369..6427
                     /locus_tag="ACHY5W_004031"
     CDS             5369..6427
                     /locus_tag="ACHY5W_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS4448814.1"
                     /translation="MLNTVKISSYELINADCLEFMRSLPENSVDLIVTDPPYFKVKPA
                     DWDNLWVGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGCEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADVTGKKNMVSHWFSASQWQLPNESDYLKLQALFVRVA
                     EEKHQRGELEWPHHQLVSTYSELNRQYVSLQEEYKTLRRYFSVSAAVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLRQIISASSRPGDLVADFFMGSGSTVKAALSLGRRAIGVELET
                     GRFEQTVREVQDLISQNG"
     gene            6468..6543
                     /locus_tag="ACHY5W_004032"
     tRNA            6468..6543
                     /locus_tag="ACHY5W_004032"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6501..6503,aa:Ile,seq:cat)
     gene            6624..6700
                     /locus_tag="ACHY5W_004033"
     tRNA            6624..6700
                     /locus_tag="ACHY5W_004033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6658..6660,aa:Arg,seq:tct)
     gene            6870..7147
                     /locus_tag="ACHY5W_004034"
                     /pseudo
     CDS             6870..7147
                     /locus_tag="ACHY5W_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT         1910 a         1606 c         1983 g         1648 t
ORIGIN      
        1 tgtattcgaa gtggacgatg acgcaacacc tgatgaaatt catgacgaag caaaagaaat
       61 tttctttaac cactgcaatt actcatatca cgaaataaaa gacgaagagg aagaacaaaa
      121 tggctgattt tggttcaact aaatataacg ccagttttga agaatggcat gaactgttaa
      181 tggactatgc agagttacgc ggtggaagtg ccgccgatgc tgaagcatgg cgtgatgact
      241 atgaagcagg aaaaactccg gtcgaagcat attgtgatga gtggggcgat gaatgagcga
      301 gattaattat caggaagggc atgaaacggc agggcaggca aaaccagtgg catggcgata
      361 tcgctacgtg aaaaaagacg ttacagactt tcaggggaag cagagggttg gtgactggaa
      421 atatgtaccg acaaaagagg attgcaacga cagaccgaac tatgaaattc aggcgttatt
      481 tacgacccag gctgtgccac tgacaccaga aggattgatt aaagcagtgc gcttctatga
      541 acaggttaag cgtgaaaatc cgccagtcga aaccggagcg tggaaagatg ccgttgattg
      601 ggtgctcaaa gaggcctgcc aggctgtaaa cattggcatc aaaggagagt gaacatggct
      661 actttgcagg aattaatcgg cctgacgcca gaacaggaaa aagcgtggaa gcgccttgtg
      721 aaggctgtaa aggatttcag ggcagccgga ggaaagtttt atagcgtcct ggacacgctg
      781 agcgcataca acggcgagca cgttgccagc attgataacg ataagggcta ccacactgca
      841 agagtctata tgcctagcat tgatgcgcca gggttaacca gttgggctga tgattggcac
      901 ggcatcacgc tgaaagatgg cgttgaagtg gatgaggact aacacatgac tacttttacc
      961 agagagcagt taatagctca cgcagaggag accattgaag cacagagact gtgcataccg
     1021 agcacaatcg accatgacat catccgcaca tataagatgg atattgctgt tctggaaatc
     1081 gcactggcat cgctggcagc agagccagtc ggtaaattgc atgaatacaa accagtgggg
     1141 tatcagcgtc tggtcgacga gttaactatg caggtaaagc agttagcctg gcaactgagg
     1201 aaagcgaagc cggactgcaa actgccggat aaggcgatgg actacctgga gcgaaacgga
     1261 ctgacaagcg tggaggatgt tttacgatga ccaggcctga agcattcaca acggcaggaa
     1321 ttgcgatggc ggtggcgctg gtggtgtatt cgatttgccg ctggggataa atcaccgtaa
     1381 aaaaaagatc ccgacacaaa catgagccgg gatctttgat ttatatagct tacgaatccg
     1441 ccagtaagag agcggggcgg atggcttatt ttaacattgg ggtggaggaa ccaaaagtgt
     1501 cgttttggta gctaaaaatt tttatccgca ttagactaat ctaaccttag tactacacca
     1561 ttaattacgc gccaggttgg tgcgttggcc ttgccaaaag aacttcaagg gaaacgacaa
     1621 gatgagctac gcactcaaga aacacacacg gctaaatatt ccgcctcgcg acaaacgagt
     1681 tgtggcagcc cctcgcccgg cgattgatga aaactgcact catcgtgagc aggtgaaaaa
     1741 tgctttcgat ttcggttttt ctcgttacga gaaggcaatg gaagaacttt caaaagtgta
     1801 atgatgggta ttgtgctcta tggctgagat tgttgaagga gtgcattacc ttacggttga
     1861 tgatcttgtt gaaatcaatc gttccctaat tgaattacag acgccagatg aacccgttgg
     1921 tgttctgagt ccagataact taagttcttc tcaggcccgt cccagcatgg ttcgatggta
     1981 taaacagatc aatgacatgt ttgtactggc atcggtattg attgaaagtc tgattcagaa
     2041 tcatccgttt gcgaatgcga acaaacgaac agctatgatg gctggttacg tcttcttgtt
     2101 gttgaatggc tatgagttaa cagcaccagg cgatgaaatc gtggaaatgg cagagggact
     2161 ggcctgcaaa acctatactc gagaagatct cgagaactgg ttgtgttatt ggtctcgtgc
     2221 atatgacagc cgggaattat gtaaaacagg cgcaactatt gttttgtatg aaactatcaa
     2281 gcttaaaata gaacagcaaa actaaaggtg cttccaatga aaacccgctt cggcgggttt
     2341 tgttttttcc ttgcattctg gtttacaatt cgcacgccag cctgaacaac tggcacctgc
     2401 tgcgtcagca gagaaaactg atggcgcacg ataccagatt ttacaattcg gataactctg
     2461 ccgcccctgc cagcaggcac gggcggcgtt ctcatgcatt caaatctgac tggttccggc
     2521 atgacccatg cactgaagaa caggccgaat ggctgattca gtgctaccgc agacgtggtt
     2581 acgagtttca gaaagatctc agcttcgatc gtcgtcactg gataatctcc gtcaggctcc
     2641 cttattccga acgcccaccg cgtccgtccc gcacattcca gcagcgcatc tggaggtaac
     2701 gtgcgggtat tacttcgacc tgttctggta ccggaactcg ggatggtgct cctaaagccg
     2761 ggccgtgaat caatgtcagc attccataac ggcagaatat tggtggagcc ggaaccgaaa
     2821 aacatgcgcg gtctgccgtc cggagtcgtt cctgccgttc gacagccgct ggcagaggat
     2881 aaaacattac tgccattttt cagcaacgaa cgggtgattt gtgctgcggg tggcgctggt
     2941 gcactgtctg actggttatt acgtcaagtg aaatcctgcc agtggccaca tggcgattat
     3001 catcacagcg aaaccgtcat tcaccgttac ggtaccggcg cgatggtgtt gtgctggcac
     3061 tgtgacaacc agctgcgcga ccagacatca gaatcactcg atcaacttgc tcagcagaat
     3121 ctggttgcct ggatgattga cgtcatccgt cacgcaataa gcggtacgca ggagagggag
     3181 ttatcgctgg ccgaattatc ctggtgggcg gcctgcaatc aggtggtgga tgcactgcct
     3241 gaggcagtag cgcgtcgttc gctgggatta ccagcggaaa aaatccgctc cgtataccgt
     3301 gagagtgaca tcgtaccggg agaacagaca gccatcagca tactgaagca gcgcacaaaa
     3361 aatattgcgc tgccacttca cgtccaccag caacaaaatc caccacagaa aaaaacggtt
     3421 gtcagtatcg ctgttgatcc ggagtctcct gaatcgttca tgaggcggcc taaacgtcgc
     3481 cgttgggtta atgagaaata cacgcgctgg gtaaagacac agccgtgtgc gtgttgtggt
     3541 aagccagctg acgatccgca tcacctgatt ggtcatggtc aggggggaat gggaacaaag
     3601 gcccacgata ttttcacgct accgttgtgc cgggaacatc acaacgaact tcatgctgac
     3661 ccgctggagt ttgagaaaaa gtacggctcc caggttgagt taatttttcg ttttcttgat
     3721 cacgcctttg caactggcgt gctcgggtaa aagaggtgac tgatgctcat agatttggtt
     3781 ttaccttatc cgccgacagt gaacacttac tggcgacgcc gtggcagcac atattttgta
     3841 tcaaaagccg gggagcgtta tcgccgggca gtggcgctta ttgttcgcca gcagcggttg
     3901 aaattaaacc tgtccggaag gctggcgata aagattattg cagagccacc ggataagcgc
     3961 cgtcgtgacc tggacaatat cctgaaagca ccactggatg cgctgacgca tgccggacta
     4021 ctcatagacg acgagcagtt tgatgaaatc aatattgtgc gcggtcaact cgttcctggt
     4081 gggcggttgg ggataaaaat cacagaactg gagtgcgcat gaataaccag tatttacagt
     4141 ttgtgcgtga gcagctcatt atcgccaccg ctgatttgag tggggcaaca aaaggtcagc
     4201 ttgaagcctg gcaagagaat gccatgttcg atacagggcg ttacaggcga aaaaaaatcc
     4261 ggtaccgcga tgaagtgact ggaaaaatga taacgcggga taatccacca atcccgggaa
     4321 agcaatcgct ggcgaagggg acgtcaattc ctctggtcag tccggttgag ttttcaacat
     4381 catcgtggcg gcgtgccgtt ctggaactgg aagagcatca gaaggcgtgg ttactgtggt
     4441 gctacagtgg aaatgtttgc tgggaagatc aggtcaccat aacacagtgg gtatggagtg
     4501 agtttaaggc tcaatccggt accagaaaaa tagcggggaa aacgcaggaa cgcctgaaga
     4561 ggttgatctg gctggctgta caggatgtga agagcgagct ggtcgggcgt gagacctatg
     4621 aacatcaggc gttggcggtg ttgatgggag ttgctaaatc tacatggaca acaacacaca
     4681 ggcctcactg gttggctatg cggggtagtg cagaggggct ggacagggat gctcttattt
     4741 cagtaatgcg ggtacgatca cggcaacagt cgcttaattt agaaccatat cttgcaaaat
     4801 agaactgaag agcatatatt tcatgtaaat ctgatatttt gccgattttg tacgcgatgg
     4861 caaagtaaga aaaaaccgcc gccaggcggt ttttttatgc ccgaaaatcg cgtcagtaca
     4921 gtaaacgcgc tggtggttgc gaatacgggt ctttcagctt gctggctttt tcgacaagag
     4981 ttattggtat gtcacgttaa ccggaaaagg gaaaaaaggc atgctaaaac agcaggatat
     5041 gacagaaacc gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg
     5101 ggagattgcg cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct
     5161 ggttatggcg ggtctggcag actatcagtt cggttgttac agacgccttc agtcctgaag
     5221 gcttttttat ttgtggtaaa tgggcggctg gtgggtgtta ggggcactca ccagccatct
     5281 gctcatgcgt ctggatcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg
     5341 agcctatcag agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttatga
     5401 gttaatcaac gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgtggacct
     5461 gatagtcacg gacccgccgt acttcaaagt gaaacccgcg gactgggata acctgtgggt
     5521 gggtgatgaa gattacctga agtggctgga ccagtgcctt gcgcagttct ggcgggtgct
     5581 gaaaccagcc ggaagtcttt acctgttctg tggccatcgc ctggcatctg acaccgaaat
     5641 catgatgcgt gagcggttta acgtactgaa ccatatcatc tgggcaaagc cgtccggacg
     5701 ctggaacggg tgtaacaagg aaagcctgcg ggcgtatttt ccggccacag agcgcattct
     5761 gttcgcggaa cattatcagg ggccgtatcg cccgaaagat gatggctgtg aggcgaaggg
     5821 cagggcactg aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc
     5881 tgccctgggg ataacggcaa aacagattgc agatgtcaca ggaaagaaaa acatggtgtc
     5941 gcactggttc agtgccagtc agtggcagtt gccgaacgaa agcgattatc tgaaattaca
     6001 ggcgctgttt gtccgggtgg cagaagagaa gcatcagcgc ggtgaactgg aatggccaca
     6061 ccaccagctg gtcagtacat acagtgaact gaaccgacaa tatgtcagcc tgcaggaaga
     6121 gtataaaacc ctgcggcgtt atttttccgt atcggctgcg gttccttata cggatgtgtg
     6181 gacgcacaaa cccgtacagt actatcccgg aaaacatccg tgcgaaaaac cggcagaaat
     6241 gctgcggcag ataatcagtg cgagcagtcg tccgggagac ctggttgcag atttcttcat
     6301 ggggtcaggt tcaacggtaa aagcggcgct gtcactggga cgtagggcga ttggtgttga
     6361 gctggagacc ggacgttttg agcagacagt cagggaagtt caggatttaa tcagtcagaa
     6421 cggatgatat tgcagaatca gtgacgcacc gttattattc tgcgcccggc cctttagctc
     6481 agtggtgaga gcgagtgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca
     6541 ccaaccgcca ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg
     6601 tggcggtcca ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct
     6661 aagcaatcgg tcactggttc gaatccagta caacgcacca cacttatttt ccaggctcgc
     6721 ttcggcgggc cttttttgta tctgcgccac gcccggcgca tatcaaccac agagcctttc
     6781 gggggggagc ttacggagtg gtcagtgtga ctttctctgt gggcagatcg ctcccgggcg
     6841 ttggctcacc cacccaaagg aacgtcacga tgtttggtat ttttggtaaa aaagcccgcc
     6901 gagcggcagt ggaaattaaa aagtttgaga aacgtgatct ggcacaggcg gttattaatg
     6961 ctgcctacct ggtggcctgt gcagatggtg aatgtgaggc ttcctagaaa gcgaagatcg
     7021 aacaggtact gcgtaatcag cctgcgctgt ccgcgtttac gtcagaaatt aatgcgatta
     7081 gcgcaaccat tatcggtcag ctggatacga actttaaaat tggtcgtcgt gcggcgttac
     7141 gtgagat
//
LOCUS       DAZUGK010000214         7105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZUGK010000214 DAZUGK010000000
VERSION     DAZUGK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.215"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(103..738)
                     /gene="msrQ"
                     /locus_tag="ACHY5W_004035"
     CDS             complement(103..738)
                     /gene="msrQ"
                     /locus_tag="ACHY5W_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFS4448815.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(739..1743)
                     /gene="msrP"
                     /locus_tag="ACHY5W_004036"
     CDS             complement(739..1743)
                     /gene="msrP"
                     /locus_tag="ACHY5W_004036"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFS4448816.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKVSGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1852..2265)
                     /gene="hiuH"
                     /locus_tag="ACHY5W_004037"
     CDS             complement(1852..2265)
                     /gene="hiuH"
                     /locus_tag="ACHY5W_004037"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFS4448817.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2398..3069
                     /gene="hprR"
                     /locus_tag="ACHY5W_004038"
     CDS             2398..3069
                     /gene="hprR"
                     /locus_tag="ACHY5W_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFS4448818.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSILEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3069..4427
                     /gene="hprS"
                     /locus_tag="ACHY5W_004039"
     CDS             3069..4427
                     /gene="hprS"
                     /locus_tag="ACHY5W_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFS4448819.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLESLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIYADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4535..5386)
                     /gene="hchA"
                     /locus_tag="ACHY5W_004040"
     CDS             complement(4535..5386)
                     /gene="hchA"
                     /locus_tag="ACHY5W_004040"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFS4448820.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(5978..7051)
                     /locus_tag="ACHY5W_004041"
     CDS             complement(5978..7051)
                     /locus_tag="ACHY5W_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS4448821.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     SDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         1946 a         1514 c         1676 g         1969 t
ORIGIN      
        1 tgatgacaaa cgaaacataa cctgatgcga tctgtgtatg ccggataggg cgctcgcgcc
       61 acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag ataatttgtt
      121 gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc gtaaggctaa
      181 aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct tcactgacca
      241 cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca actgttgcca
      301 gtgcttgccg agttttcgct gcatcgcctg ggttgaagtg aacgctaaag caagcaaaat
      361 tacccagctg ataataccta acgttaaata aggccgggta attaactcct tacctaataa
      421 cgccagatta ttcacgccca gctccagcaa tgcgtaactt gttaaatgca gtgtcgccca
      481 ggcaaagcac cataatccta acaggcggcg agtgcgtatc aataacggct gttttgcgta
      541 gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccggt
      601 aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga tcgcccagac
      661 cagccagaga aacggcaaca atccggcaag atgcaggcaa actttcagcc atgtcacctg
      721 ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat acagcgatgc
      781 cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat cgaggatgcc
      841 gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa catgcggatt
      901 aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg
      961 ctcgcgggtc agcttaatac tgacgatcga tttaatccct ttaaagccat atttccacgg
     1021 cacaatcagt cgcaccggcg cgccattttg tggcggtaac gccttgccat aaacacctac
     1081 ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata
     1141 tttcagcccg ccgccgataa agcggtcctg ctggcctggc atctgttccg gtgcataaat
     1201 tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg
     1261 cagcggaaaa ccaatccacg gcaccaccat cgaccacgct tccacgcagc gcatacgata
     1321 aatacgctct tccagcggga agcgacgggt taagtcatca tgatcgaggg tcaatggttt
     1381 tgccacttcg ccgctgactt tcagtgtcca tggatcggtt ttcaggctac cagcattagc
     1441 ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg agactttatc
     1501 tgctggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcgcttt
     1561 tccggcgggg gcgggtgggc gatcgttccc tttaaaccag ctaagcagat cggcatgcgc
     1621 agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca cctgccgacg
     1681 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
     1741 cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt taatctgtca
     1801 gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc attaactgcc
     1861 acgataggtt gaatacccat attggctaag taataaaggc acatgataat gctcgttcac
     1921 tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat tttgtttctt
     1981 gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag tttgctcggg
     2041 ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt gtaaccagcc
     2101 gttgtccgct ttcttttcaa gagtgactgt cacgtcggca gcgggttttc ctgtttgctg
     2161 gttcaaaatg tgcacgctaa gaatgttttg ttgtgcagca tagaccaaag aaggtaatga
     2221 aaatgcagcc gttactacgg agagtactaa ataacgcttt aacatgttta tatccttgtc
     2281 atgtgaatga gttttgagga tagcgaaaaa gcgtccaggg taaccttaca gcagcattac
     2341 aattttgtaa tgaaccaggc tgtttctata acatatgatt tatggcatat tattttcatg
     2401 aagattttac ttattgaaga taatcaaagg acccaggagt gggtaacgca ggggctttcc
     2461 gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta tcttgcgctg
     2521 aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga tggctggcag
     2581 atcttacaaa cgttaagaac ggcaaagcaa acccctgtta tttgccttac tgcaagggat
     2641 tctgtcgatg acagagtcag agggctggac agtggagcaa atgattatct ggtaaaacct
     2701 ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taaggcaaca tcacgctttg
     2761 aattcaatat tagaaatcag cggcttaaga atggactctg ttagtcaaag tgtgagcagg
     2821 gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct actggcctcc
     2881 agagctggcg aaattatacc cagaacggtt attgcgagtg aaatttgggg aatcaacttt
     2941 gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa agttgatgat
     3001 ccttttcctg aaaagctaat tgccacaatc cgtgggatgg gatattcgtt cgtagcggta
     3061 aaaaaataat gaaaagattg tctataaccg tccgtttaac cttgcttttt atattgctgc
     3121 tgtctgttgc tggcgctgga attgtctgga ctctctataa tggcctggca agtgagttga
     3181 aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag ttgttaattg
     3241 atggggtaaa tccagatacg ttacctgtgt actttaaccg gatgatggat gttagtcagg
     3301 atatcttgat cattcatggt gatagcatca ataaaattgt taaccggaca aatgtcagtg
     3361 atggcatgtt aaataacata cctgctagtg agacaatcag tgcagctggc atttacagaa
     3421 gcattattaa tgatacagag atagatgctt tacgaattaa tattgatgaa gtttcgccat
     3481 cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt gaacagtata
     3541 aaatcaatag cattataatt tgcattgtcg ccattatact gtgctcagta ttaagtccgc
     3601 tgttaatccg aacggggtta cgagagatca aaaagttgag tggtgtaacg gaagcgctga
     3661 attataacga tagccgagag cctgttgagg ttagcgcatt accgagagaa ctaaaaccgc
     3721 ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa agattttgag cgtctaagtc
     3781 agtttgctga cgatctcgct catgaactta gaacgccaat taatgcatta ctgggtcaga
     3841 atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca attgccggaa
     3901 acattgaaga gctggaaaat atttcgcggt taacagagaa catactgttt cttgccaggg
     3961 cagataaaaa taatgttttg gtgaaactgg aatcgctttc tctcaataag gaagtcgaaa
     4021 atttgttgga ctatcttgaa tacctttcag acgagaaaga gatttgcttt aaggtcgagt
     4081 gcaatcagca aatttatgcg gataaaattt tactgcaacg aatgttatcg aatcttattg
     4141 ttaacgccat tcgatattca ccagaaaaat cgcgcattca tataaccagt tttcttgata
     4201 ctaacggatc tcttaatatt gatatcgcca gccctggaac gaaaattaat gagcctgaaa
     4261 aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt cagggactag
     4321 gcctttcttt agtcaaagcg attgccgaat tacatggggg aagtgctacg tatcactatc
     4381 tcaataagca taatgtgttc cggattacgt tgccgcaaag aaattaacat agggcttcag
     4441 tacgccataa attaaccgtg aaaaaacgca gccacgtcgt gaatactaac gcggctgcga
     4501 ttgattatgc gcttacatta aaacgtaaca gggattaacc cgcgtaagct gccagcattt
     4561 cctgcgccgc cagtttaccc aacgcattcg ctgcgaaagg actgtcgccg gtgagaactt
     4621 tacggtcctt atgtactcgc ccggtgatgt cgtcattaat aatgttcatg cccattttct
     4681 tcagctcttc gccgaagtac caggtgagat gacccggcat atagcctatc tctggcgttt
     4741 gtttgtctgc ggcgtctggg aatgcgcaaa tggaataacc attcagtggg ttatcgccgt
     4801 ggcgaagcgc cagaaaagcc gccgggccgt ggcaaaggga gataacaaaa cggtcatttt
     4861 tgattgccca ctgtaaagcg gcagctacgt cctggctttc aggtaaacca ataagtgcgc
     4921 catgaccacc aggaacaaag attgctgcat attcgctatc agcgttgagg ctggcgacaa
     4981 catccgcgag tttcttcgga ttgcggaaca acgatttatg ctgctcaaag aatggcatta
     5041 ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga ccggaaatgg
     5101 tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc aacgtttcaa
     5161 tcggatggtt accggtcgag aacagttttc cgttatcggt tggcaaataa cgttcgtccg
     5221 cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc acgccatcaa
     5281 gatcagagac aggactggta tattggctaa gcgaatattc tgaagggaag aatgcattat
     5341 cttcagcaat atcgacctgc ggatttttac ttgtttgaac agtcatagtg tattccttat
     5401 tgttgcttag ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag
     5461 gctggaaaat tgagtcaata tatcgaaagc ggtagtcggc gggtgtaagt atgtaatgat
     5521 gatgctgcac tggtgttgaa tattattcac gccaaatttt ccttattgag caacgtcgtc
     5581 gcaagtggcc tcaatgattt tagaccatta tcagaggtac ggggcggcgg ggagagattt
     5641 agtgatgtgg aatattgtgg tgctgatgaa tatttatatc ccggattggt agagaaaaag
     5701 cttatttcat gcactatttt tgcaaaaatt tctatatggg aagcgtgttt gttacggatt
     5761 aattcataaa gaaatgcatt tgggtgttag cttgaagagt agctggttga tgttattgtg
     5821 ccaggagggg cagggtaagg gggtgtatcg ataagctttg gcaattgcgt gtgccgcaga
     5881 tggagatgaa atgccaataa aaaagcctga gttcgatgag ggacgaactc aggctagtgt
     5941 tcagttttac agtaacacgc cgcggcgcgt tggctgatta gaactggtag acgataccaa
     6001 ctgcaaactg gtcatcagtt gcggcatcgg taatcgctgc atcgtcttta tccagcaggt
     6061 taaatttgta tgctgtgtag acgttcatgt tcttattgaa gtagtaccag gtaccaactt
     6121 cgatataatt tacgcggtca gcatcaacac ctttatgtcc atcacgagca taaagatctt
     6181 tgcctttgga atatacgtag cctaaagacg gacgtaaacc gaagtcgaac tgatactgta
     6241 caacagcttc aaagttttgg gttttatttg caaaatgatc attagccaaa acggtcatgt
     6301 tgcgggtttc accgtacatc attgctgcat agatattatt tgcatcatac ttggctgcta
     6361 atgaccacac ttcagcgtta tcaccgtagc cgtcactagc ttgttcatta gtacgatcag
     6421 atttgctata tgcacctaca aaaccaaaac cttcgatgtt gtagtctaca gacaacgcat
     6481 agccgtcacc attttgtttc ttagtgctac ggttgctgtt tttgccttgg tactgaatcg
     6541 caaaatcaag accatcaacc aggccaaaga aatcatagtt acggtaggtt gcaacaccgt
     6601 tggtacgacc attcatgaag ttgtcagcgg aagcccagga atcgccaccc cattcaacta
     6661 acatatcggt ataagctgcg gcatcataag caacaccgta gttacggcca tagtcaaatg
     6721 atcctaactc gttatatttc aaaccggcga aggttaaacg ggttttttcc tggttttcac
     6781 cttcgggctt gctggcgtcg aggttgtatt caaactgacc aaaaccagtc agttcgctgg
     6841 tgatttgggt ttcaccttta acaccgaaac gagcatagga ggtatcttcg ttttcgctgt
     6901 tattatcatc agtatttgac cagatgcgtt cgccaaccat cttgccgtag aaatcaactt
     6961 tattgccatc tttattataa atttcagccg catttgctgc gccagcaact aataacgccg
     7021 ggaccagcat tgctagaact tttcttttca ttatgtattc ccttgtgatt ataatcttca
     7081 tgaatatatc aataagtgcc gttat
//
LOCUS       DAZUGK010000215         7096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZUGK010000215 DAZUGK010000000
VERSION     DAZUGK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.216"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            285..1199
                     /gene="shuT"
                     /locus_tag="ACHY5W_004042"
     CDS             285..1199
                     /gene="shuT"
                     /locus_tag="ACHY5W_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFS4448822.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKSTVKRKKLFTALLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRMERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1219..2556
                     /gene="hutW"
                     /locus_tag="ACHY5W_004043"
     CDS             1219..2556
                     /gene="hutW"
                     /locus_tag="ACHY5W_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFS4448823.1"
                     /translation="MNANNPLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDGCLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWEEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLEELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPASVREKP"
     gene            2569..3063
                     /gene="hutX"
                     /locus_tag="ACHY5W_004044"
     CDS             2569..3063
                     /gene="hutX"
                     /locus_tag="ACHY5W_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFS4448824.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3063..3686
                     /gene="chuY"
                     /locus_tag="ACHY5W_004045"
     CDS             3063..3686
                     /gene="chuY"
                     /locus_tag="ACHY5W_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFS4448825.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNKQIYSLIEPDLKPA"
     gene            3737..4729
                     /gene="shuU"
                     /locus_tag="ACHY5W_004046"
                     /gene_synonym="chuU"
     CDS             3737..4729
                     /gene="shuU"
                     /locus_tag="ACHY5W_004046"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFS4448826.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLSVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4726..5496
                     /locus_tag="ACHY5W_004047"
     CDS             4726..5496
                     /locus_tag="ACHY5W_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFS4448827.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYQQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLEDPANHSPLIVLDH"
     gene            complement(5548..6195)
                     /locus_tag="ACHY5W_004048"
     CDS             complement(5548..6195)
                     /locus_tag="ACHY5W_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4448828.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6259..6585)
                     /gene="hdeB"
                     /locus_tag="ACHY5W_004049"
     CDS             complement(6259..6585)
                     /gene="hdeB"
                     /locus_tag="ACHY5W_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFS4448829.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6701..7033)
                     /gene="hdeA"
                     /locus_tag="ACHY5W_004050"
     CDS             complement(6701..7033)
                     /gene="hdeA"
                     /locus_tag="ACHY5W_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFS4448830.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1782 a         1788 c         1713 g         1813 t
ORIGIN      
        1 cttgttttta tggttatccc cccatgcctc atcactaaat tttgtcgtta acagtgataa
       61 ttttttcttc tctttgctct gcaagcctcg atatctctgg gttctcagct ttttcccgcg
      121 atgaaattta gtcggtaaca agaacgttag ctgacgaagc atcttgataa aaatcaaaaa
      181 taaatacgct tgtaacgcct tgcgtattga atcgacggtt gtatttcata tataactcaa
      241 atgataatca tgatcattct caatttgata agagttctca tattatgcca aggatcatca
      301 ccaggccgtt tcttttttcc ccactgacgt tatgtatttc agccgtggcc tctgccgcga
      361 agagcacggt aaaacgaaaa aaattgttta cggcattact ggctcttagc tgggctttta
      421 gcgtaacggc tgccgaacgt atcgtggtcg caggaggatc gctgacggag ctaatctacg
      481 cgatgggcgc tggcgaacgc gtggttggtg tcgatgaaac gacatcttat ccaccagaaa
      541 ccgccaaact gccgcatatt ggctactgga aacaactcag cagcgaaggc attttgtcac
      601 ttcgcccgga tagcgtaatt acctggcagg atgccgggcc acaaattgtg ctcgaccaac
      661 tacgagcgca gaaagtcaac gtcgtcaccc tgccgcgtgt gcctgccacc cttcagcaga
      721 tgtacgccaa cattcgccaa ctggcaaaaa cgctacaggt tcctgaacaa ggcgaggcgc
      781 tggtgacaca aatcagccaa cgcatggagc gagtacagca aaacgtggcg gccaaaaaag
      841 ccccggttaa agccatgttt attctctccg caggcggaag tgcgccacaa gttgccggta
      901 aaggcagcgt cgcggacgcc attctttcgc ttgccggagc agaaaacgtc gctccccacc
      961 agcagtacaa aagctacagt gcagaatcac tgattgcggc taaccctgaa gtgattgtcg
     1021 taacctcaca aatggtcgat ggcgatatta atcggctacg ttctattgct ggaattaccc
     1081 acactgccgc atggaaaaac cagcgcatta tcaccgttga tcaaaacctg attctgggaa
     1141 tgggtccgcg tattgctgat gtcgttgagt ctttacacca gcagctttgg ccgcaataaa
     1201 tggatttaaa tcagacgaat gaacgcaaat aaccccctgg atctcacgcc acattttgct
     1261 ttagacggtg atcagccatt taaagacagg cgcgccatga tgccgtttcg tggggccatt
     1321 ccagttgcca aagagcaact ggcacagacc tggcaagaga tgatcaatca aacggcatca
     1381 cctcgtaagc gactggttta cctacacatt cctttttgtg cgacacactg cacgttttgt
     1441 ggcttttatc agaaccgttt taatgaagat gcgtgtgctc attataccga cgccctgatt
     1501 cgtgaaatag aaatggaagc ggacagcgta ttgcatcagt ctgcccccat ccacgcggtc
     1561 tatttcggtg gcggtacgcc ttccgcgctt tcggcacacg atttggccag aatcatcacc
     1621 acattacgag aaaagctgcc actggcccct gattgtgaaa tcactatcga aggccgggta
     1681 ctgaattttg acgctgagcg aatcgatggt tgtcttgatg caggagcaaa ccgcttctca
     1741 attggtatcc agtcgtttaa cagcaaaatc cgcaagaaaa tggcccgcac ctcggatggc
     1801 ccaaccgcca ttgcgtttat ggaaagcctg gtcaaacgtg accgtgctgc ggtggtctgt
     1861 gacctgctgt ttggcctgcc gggtcaggat gcgcaaacct gggaagaaga tctggctatt
     1921 gcccgcgata tcggtctcga tggcgtcgat ctctatgcgc tcaatgtcct gcccaataca
     1981 ccgctgggca aagccgtgga aaatgggcgt actaccgtgc cctctccggc agaacgtcgc
     2041 gatctttatc tgcaagggtg tgacttcatg gacgaagccg gttggcgctg tattagtaac
     2101 agccactggg gccgtaccac acgcgaacgc aatctctata acctgctgat aaaacaaggt
     2161 gccgattgtc tggcctttgg ttccggagcc ggcggttcga ttaatggtta ctcctggatg
     2221 aacgaacgca atcttcagac ctggcatgaa tccgtcgcgg caggcaaaaa accgctgatg
     2281 atgatcatgc gtaacgccga acgtaatgcg cagtggcgtc ataccttgca gtcaggtgtt
     2341 gaaacagcgc gtgtaccgct ggaggaactt acaccacatg ccgaaaaact cgcgccgtta
     2401 ctggctcaat ggcaccaaaa aggtttaagt cgcgatgctt caacttgcct gcggctgact
     2461 aatgaaggtc gtttctgggc aagcaatatt ttgcagtctc ttaatgaact aattcaggta
     2521 cttaatgcgc cagcgagtgt gcgtgaaaaa ccataacagg agtgatttat gagccatgtc
     2581 tcgttacaag aatttttgaa aacggagccg gacggtacgc tggaggtggt agccgaacaa
     2641 tacaacacca cactgctgga ggtagtgaga aatctaccct cctctaccgt agtgccaggc
     2701 gataaattcg atactgtctg ggataccgtc tgcgaatggg gcaacgtcac cacgttagta
     2761 catactgccg atgtcatcct cgaatttagc ggcgaactgc cttcgggttt ccatcgccac
     2821 ggctatttca acctacgcgg aaagcacggc atgtccgggc atatcaaagc agaaaactgc
     2881 acgcatattg ccttaatcga acgtaaattt atgggcatgg acaccgcgtc cattctcttt
     2941 ttcaataagg aaggcagcgc catgctgaaa atcttcctcg gcagggatga ccatcgtcag
     3001 ctcctgagcg aacaagtcag cgccttccat actctggcgg catcgttaaa ggaacacgcc
     3061 tgatgacgcc gtggctactc tttggcgcag gcggaaaagg tgtgggtgcc agaacgcttg
     3121 aactggcgct ggcggaacaa cgtccggtcg ttgctgtcat tcgtcatgcc gatgccgcca
     3181 cgaaactggc gcaacaaggt gtacaggttt ttacaggcga cgcctgtgat gccagcgtag
     3241 tcgcggctgc ttgccgcgca gctggcccgg atgcgcttat catctcaaca atgggaggag
     3301 ctcaggatta tctggcgcac agaacagtga ttgatgaggc cgaaaaggcc ggcatcagtc
     3361 gcatgattct ggtgacttct ttgggatgcg gcgatagttg gccgttctta tcggatcggg
     3421 caaaagccgc gtttggtcag gcggtgcgcg aaaagacact ggcggaaagc tggctgcaaa
     3481 ccagccagct agactacgcc attctccgcc ctggtggtct gcttgacggc gcagcaacag
     3541 gcaaagcaca acgggtacaa aatcaggagt gtcatggttt tgtccgccgt gccgatgtcg
     3601 ctgcacacat tcatgaactg gcaaacgcgc cagcactcaa caagcagatc tacagtctga
     3661 ttgaacctga cctgaaaccg gcgtaatccc atgcggcgca gactctccgc gccgcaacgt
     3721 cataacggag ttttgcgtgc tcaaggattc attttcatcc gccagggtct ttatggggct
     3781 atcactatta ttgcttgccc tggtgctgtt cggtgccagt cagggagcgt taaagatcag
     3841 ttttgatgcc ctttttgatg aggaataccg cgatatctgg ctcaatattc gtctaccacg
     3901 ggttttgctg tcggtgctgg taggtgcagc gttagcaacc gcaggggtaa ttatgcaggg
     3961 gctttttcgc aacccaatgg ctgaccctgg attactcggc gtcagtagcg gttctgcatt
     4021 aatggttggc gttgctattg tgctgccctt ctccttccca gtggtgctgg tgctctatga
     4081 gcaaatggtg tttgccattg ccggaagttt agtagtctgc accatcattt ttctcatcac
     4141 gcagcgccat cgcgatggca gcatgatgca attattactc gccggtatcg ccatcaatgc
     4201 cctgtgcggc gcagcgatcg gcatcctgag ctatatcggc gatgagcagc agctacgaca
     4261 actcacattg tggatgatgg gcaatcttgg acaggcgcaa tggccgacgt tattggttgc
     4321 cagttcattc atccttccgg ccattatcgc aacaacttgt ctcgccggaa cgctgaattt
     4381 actgcagctc ggtgatgaag aagcccatta cctcggcgtg aacgttaagc gtaaacgcca
     4441 gcaattactg ttagtgagct cactgctcgt tggtgctgcc gtatcggtaa gcggcattat
     4501 cggctttatt ggcctggtga tcccgcatct gattcgcatg actaccggag caaatcaccg
     4561 ctggctaatc ccttgttccg ccctcgccgg tgcctgttta ttgctgatgg cagacacact
     4621 tgcccgcacg ctggtacagc cagcagaaat gcccgtggga ttattaacca gcctgcttgg
     4681 tggcccttat tttatgtggt tgattctgcg aaatcggagg atcacatgat cagcgcccag
     4741 aatctggttt atagccttca ggggcggcgt ctgactgata atgtctcgct gacttttccc
     4801 ggtggggaaa ttgttgccat tctcggccct aatggtgcgg gaaaatctac gctgcttcgc
     4861 cagttaaccg gctatcttca acctgatagc ggcgaatgtc ggttgtttaa taagccatta
     4921 aatgaatggt cgattactga actggcgaaa caccgggccg tgatgcgtca aaatagccac
     4981 atggcgtttc cattcagcgt acaagaagtt atccagatgg gtcggcatcc gcatcgtact
     5041 ggtaatcagg ataatgaaac agcacaaata atggcgttat gtgactgtca ggcgttagca
     5101 aaccgcgact atcagcaatt atccggtggt gaacaacagc gcgtgcaatt agcgcgcctg
     5161 ctggtgcaac tctgggaacc tactccctca ccgaaatggt tatttcttga tgaaccaact
     5221 tcggcgctgg atattcacca tcagcagcac ttgtttcgtt tattacgcca gttagttcac
     5281 gagcggcagt tcaatgtctg ctgcatattg cacgatctaa atcttgccgc gcgttatgca
     5341 gaccgtgttg tattaatgca aaaaggaaag gttatcgcaa atggtaaacc gcaggacgta
     5401 ttgacacagc aggcgctgac aatgctctac ggcgcggata ttaccgtact tgaagatcct
     5461 gccaaccatt caccactgat cgtcctcgat cattaaaagc aacaggagta ccgcactggt
     5521 actcctgcga ataaataact atacagatta gctaattgaa acgcctttca cccctgccat
     5581 accttttaat aatcgcaaca gatcttctat cgacgttgtg gcatgaagct ggatatcaat
     5641 ggcgaccact tcatgatctt cattttcctg caacgaaact aaatcagttt ttattttttg
     5701 ctgcttaaac cagtcgagca ttgataccgt attaccgttc actagtgtca gctgtaaatg
     5761 gtaatttttg ttcatcaggc ggaaggttaa ttgatggaag acttccagca ccagcaaggt
     5821 catcactgaa ccataaatac ctagttcgta cataccgctg ccaataacca taccaatggc
     5881 ggcggtcacc cagatatccg ctgccgtcgt cagacctaca atattttggt tacgaacgag
     5941 aatgttacct gcaccgataa acccgacgcc cgtcaccacc tgagcagcaa tacggctggg
     6001 gtccagtccg acgtgatcta aagacagcac gtcagcaaaa ccatatttcg aaacaatcat
     6061 aaacagggcg cttcccatgc caattaatac atgtgtgcgt aaccctgctc ctttgccgcg
     6121 catttgcctt tccatgccaa tagcgccaca ggcaattgcc gcgagtataa gacgtataat
     6181 aaattctgct gtcatataaa aatgcaaaaa ggagcagcaa gatggctcaa cttgctactc
     6241 ctttttactt gtacctcatt aattcggcaa gtcattagat gcttgatttt tgaaggtata
     6301 caaatttttc tgcgggtttt tcttacagta ttcgatcact ttaggaattt gagtgagatc
     6361 ggtttcattt aaagtaacgg tatcgccacc tttatatact gtttcttcat gcagcatcca
     6421 ccatgcaacc ggggtcattg cttttggatt cagatcaata aattcctggc aggtcatatc
     6481 tttagcggat tcattggctg ccagcgcaga ttgtgcgttc accagtgaca aagccgctac
     6541 agcgcccata aaaataaacg ctttacggag agatgaaata ttcattttgt aacccattca
     6601 atatagagat tatatatgaa caggaggaat agctgctttt aaagaagata ttccggcctg
     6661 ttcaatatgc aaggaagtac gatgtaacgc attcccggaa ttacatatct ttcttaattt
     6721 tgtcccattc gcctttaact ttatctttaa agttggcttg tttatcctga gtacaagcct
     6781 gaacgatagc tggggttacg gttgcaatac cctgaacatc taaaaccgca tcttctggtt
     6841 tatctttgtt gttcagcgct tcagcaaaac caactgcagt tggctggaag gattcgtcca
     6901 cagccaggaa atcttcacag gtccaggagt tgaccggttt tttgttatca gctgcttttt
     6961 gcgcatccgc tgcattgctc acaactggca gaagaagcag accaccaaga ataacgccta
     7021 atactttttt catcgtaata tcctcaacta taaagtgaaa gagccgtcac gaatcaattt
     7081 cgacactgag gttata
//
LOCUS       DAZUGK010000216         7073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZUGK010000216 DAZUGK010000000
VERSION     DAZUGK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.217"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..5321
                     /locus_tag="ACHY5W_004051"
                     /pseudo
     CDS             144..5321
                     /locus_tag="ACHY5W_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            5532..5798
                     /gene="hipB"
                     /locus_tag="ACHY5W_004052"
     CDS             5532..5798
                     /gene="hipB"
                     /locus_tag="ACHY5W_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFS4448831.1"
                     /translation="MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5850..6263
                     /gene="hipA"
                     /locus_tag="ACHY5W_004053"
                     /pseudo
     CDS             5850..6263
                     /gene="hipA"
                     /locus_tag="ACHY5W_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            6480..6766
                     /locus_tag="ACHY5W_004054"
                     /pseudo
     CDS             6480..6766
                     /locus_tag="ACHY5W_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1951 a         1493 c         1908 g         1721 t
ORIGIN      
        1 gcattagcaa attagccaaa ttgacacctt cccacctcca actaacactc aatacagacc
       61 tctgacaatt taataagttt catttttaat gtctggcgac tcatctcttt cgtggctgta
      121 ttgtttgtat atggagcgtt tatatgaata gaatctatcg cgtgatatgg aattgcactc
      181 tacaggtatt tcaggcctgc tctgaattaa ctcgcagggt aggtaaaaca tcaacggtta
      241 atttgcgtaa atcctctgga ctgacaacga aattcagtag attgacgctg ggtgttttgc
      301 tggcacttag cggttcagtg tctggtgcga gtctggaagt tgataatggt cagattacca
      361 atattgatac tgatgttgct tatgatgcct acctggttgg ctggtatggc actggagtgc
      421 ttaatatttt ggctggcggt aatgcctcct taaccactat tactaccagc gtcattggcg
      481 gtaatgagga ttcggagggt accgttaatg ttttgggtgg cacctggcga ttgtatgata
      541 gcggaaataa tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg ctgaatatta
      601 aacagaaggg tcacgtcgat ggaggctatt taagattagg tactcaagct gcaggcgtcg
      661 ggacggttaa tgttgaggga gaggactcgg ttttgacgac cgaattattc gaaataggga
      721 gttacggtac gggctcatta aatattacgg ataagggtta cgtcacgagt tcaatagtcg
      781 ccattttagg atatcaagcg aacagtaatg gtaaggttgt cgttgaaaag ggtggcgagt
      841 ggctaataaa aaataatgat tcctcaattg aatttcaaat tggtaatcaa ggaactgggg
      901 aggcgactat tcgcgagggt gggctgatta cggctgaaaa tacgattatc ggtggcaatg
      961 ccaccggtat cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac
     1021 tctataatgg atatttcggt aatggcaaag tcaatatttc caataatgga ctgattaata
     1081 acaaagaata ttcattggtg ggcgttcagg acggttccca cggtgtcgtc aacgtgaccg
     1141 ataaagggca ttggaatttc ctcggaacgg gcgaagcttt ccgctatatc tatatcggtg
     1201 atgctggcga tggtgaactt aatgtctcga gtgaaggcaa agtagattcg ggaattatca
     1261 ctgcggggat gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta
     1321 tcactaatct cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg
     1381 aggggcttgt tgtcagcaac ggaggaagct cactcggtta tggagaaacc ggcgtcggga
     1441 atgtcagcat caccacgggg gggatgtggg aggtcaataa gaatgtctat accaccattg
     1501 gtgttgcggg tgtcggaaac ctcaatatta gcgatggcgg taagtttgta tcgcaaaata
     1561 ttactttttt gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgcgacat
     1621 catcgttcga tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca
     1681 gtaatggtgc cacccttaat tcaacgggct atggatttat cggaggaaat acctccggta
     1741 aggggatagt taatatttca acggatagtc tctggaattt aaagacatca tctactaacg
     1801 cccaattgct acaggtcggt gtattaggca cgggtgaact gaatattacc accggaggta
     1861 tatatagtta aagcgcgtga tacacagata gctctcaatg acaaaagtaa gggcgacgtg
     1921 agggtggatg ggcagaactc tcttcttgaa acattcaata tgtacgtagg gacatctggt
     1981 acgggtacgt taaccctgac gaatagcggc acgctgaatg tcgaaggtgg agaagtttac
     2041 ttaggtgttt ttgaacctgc tgtaggatcg ctaaacattg gtacagctca cggtgaggcg
     2101 gcggcagatg ccgggtatat aaccaacgcg acgaaagtag agtttggttc tggcgaaggt
     2161 gtttttgtct ttaatcatac taataacagt gatgccggct accaggtcga tatgctgatt
     2221 acaggtgacg ataaagacgg aaaagtgatc catgatgcag gccatacggt gttcaatgca
     2281 gggaatactt atagcggtaa aacgctggtc aatgacggcc tcctgaccat tgcgtctcat
     2341 acggcagatg gggtaacggg catgggttcg agtgaagtaa ccattgcaag ccccggtacg
     2401 ctcgacattc tcgcatcaac gaacagtgca ggagattaca cgctgaccaa tgcgctcaaa
     2461 ggcgatggct tgatgcgagt gcagctgtca tcctccgaca agatgtttgg ctttacccat
     2521 gcaacaggga ctgaattcgc cggtgttgcc caagtgaaag acagtacctt cactctggaa
     2581 cgcgacaaca ccgctgcgct tactcacgcg atgttgcagt ctgacagtga aaataccaca
     2641 tcggtaaacg tgggagagca atccattggt ggactggcca tgaatggcgg tacgctcatt
     2701 ttcgatacgg atattcctgc tgcgacgctt gcagagggat atatcagcgt cgatacgctg
     2761 gttgtcggcg cgggtgacta cacctggaaa ggacgtaact atcaggtaaa cgggacgggc
     2821 gacgtgctta tcgacgtgcc taaaccgtgg aatgatccca tggcgaataa ccctctgacg
     2881 acgctcaatt tgctggaaca cgatgataac catgtcggcg ttcaactggt gaaggcgcaa
     2941 acggttattg ggtcgggtgg ctcattaacg ttacgtgatt tacagggcga cgaggtggaa
     3001 gcggacaaaa cgttacacat tgcgcaaaac ggaacggtgg tcgccgaggg tgattatgga
     3061 ttccgcctca cgaccgcacc aggtgatggt ttgtacgtta actatgggct gaaagcgctg
     3121 aacatccatg gagggcaaaa gctgacgtta gccgaacatg gcggagccta tggcgcaacg
     3181 gccgatatgt cggcaaaaat cggtggtgaa ggggatctgg caatcaatac ggtgcgacag
     3241 gtttcgcttt ccaacggtca gttacagggg gaacgctggc tatcgagggg cttaatgcac
     3301 gctacaatgc gttaaccagc gttagcccaa atgcggaagt cagcctcgat aatacgcagg
     3361 ggttaggcag aggaaatatt gccaatgacg gtctgttaac gctaaaaaac gtgactggcg
     3421 aactgcgtaa tagcataagc gggaagggta tcgtgagcgc aaccgccagc acagatgtag
     3481 agctggatgg cgataatagc cgctttgtgg ggcaattcaa cattgataca ggcagcgcgc
     3541 tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt tatcaataat ggcctgctca
     3601 tcatctccac tgagcgtagc tgggcgatga cgcacagtat cagcggtagc ggtgatgtga
     3661 caaaacttgg taccgggatc ctgactctta acaacgattc cgcggcgtat cagggtacga
     3721 cggatatcgt gggtggtgaa attgctttcg gttccgactc tgccattaat atggcaagtc
     3781 agcacattaa tatccataac agcggtgtga tgtcgggaaa cgtcaccact gcaggtgatg
     3841 tgaacgttat gtctgggggg acactgcgtg tcgctaaaac cacaatcggc ggcaacctgg
     3901 agaatggcgg cacggttcaa atgaatagcg aaggggggaa accggggaat gtactgaccg
     3961 ttaacggtaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg ctgggcggcg
     4021 ataattcacc caccgataag atgaacgtga aaggcgatac ccaagggaac actcgcgttc
     4081 gggttgataa cattggcggc gtcggtgcgc aaacggtcaa cggtattgaa ctcattgagg
     4141 ttggcggtaa ttctgcaggt aatttcgcgc tgaccaccgg aactgtcgaa gctggggctt
     4201 acgtctacac gcaggctaaa gggaagggga atgacgagaa aaactggtat ctgaccagta
     4261 aatgggacgg cgtaacgcca gcggatacac ccgatcccat caataatccc cctgttgtgg
     4321 atccggaagg cccatcagtt tatcgtccgg aggccggaag ctatatcagc aacattgccg
     4381 cagccaactc gctgtttagc catcgcttac acgaccgtct gggtgagccg cagtatacag
     4441 attcacggct ttctcagggt tcggcaagca gtatgtggat gcgtcatgtc gggggacacg
     4501 aacgtttcag gaccggtgac ggccagctaa atactcaggc taaccgctat gtattgcagc
     4561 taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat cttggcgtga
     4621 tggcaggcta cgccaatcag cacagtaata ctcagagtaa tcgtgtgggt tataaatcgg
     4681 atgggcgcat cagcggttac agcgctgggc tgtacgcgac ctggtatcag aacgattcga
     4741 ataagaccgg cgcttatgtt gactgctggg cgctgtataa ctggtttgat aacagcgtca
     4801 gttccgataa ccgttctgct gacgactatg attctcgcgg tgttacggcc tctgttgagg
     4861 gtgggtatac ctttgaagcg ggaacattta gaggcagcga agggacgctg aatacctggt
     4921 acgtccagcc acaggcgcaa atcacctaga tgggtgtgaa agattctgac cataaccgga
     4981 aagacggaac acgcattgaa acggaaggcg acggaaacgt gcaaacgcga cttggggtga
     5041 aaacctacct gaacagccat caccagcgtg acgatggtaa acagcgtgag ttccagcctt
     5101 acattgaagc gaactggatc aacaatagca aagtctacgc cgtgaagatg aatggtcaaa
     5161 ccgtaggccg tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag
     5221 taaataacaa ccttagcctg tgggggaatg tcggtatgca actaggtgat aaaggctata
     5281 gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa gccgcatgtc
     5341 gacatggcat gcggcttaat attgccaact tcaaaaggct tttaaagcct cccttttatc
     5401 cgcgatcgcg gatatcgcag cgtttatccc gtacagcgga taagatgtgt ttccagattg
     5461 actttatcct cactaaagga taaaactgat aatatcccct taagcggata aatttgctgt
     5521 ggacgtatga catgatgagc tttcagaaga tctatagccc aatgcaatta gcgaatgcaa
     5581 tgaaactggt tcgccagcaa aatggctgga cgcagagcga gctggcgaaa aaaatcggca
     5641 ttaagcaggc gacgatttcc aatttcgaaa acaaccctga caataccacg ctcacgacat
     5701 tttttaagat tttacagtca cttgaactct caatgacact atgcgacacg aaaaatgcct
     5761 cgccagaatc aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat
     5821 gaacaaccag cgggtaggcg agttaacgaa tacccgaaga cgaaaccgta acgcgtccga
     5881 taatggcatg ggaaaagctt actgaagcca gacttgaaga agtattaacg gcttataaag
     5941 cagatatccc gctaggcatg attagagaag aaaatgactt tcgcatctcg gttgctggcg
     6001 cacaggagaa gacagcgctg ctcagaatag gcaatgactg gtgcattccg aaaggaataa
     6061 cgccgacgac gcacatcatt aaattaccga ttggggaaat caggcagccc aatgcgacgc
     6121 tcgatctcag ccaaagcgtt gataatgagt attactgtct gctgctggcg aaagaacttg
     6181 ggttgaatgt tccggacgca gaaatcatta aagcgggaaa tgtgcgtgcg ttagcggtcg
     6241 aacgttttga caggcgttgg aatactacta gattctccct tggtttctgt tggtgcgcat
     6301 aatgtatatt atgttaaatc gcatctgagt gatacgaagt gattttccgg tagtcgttat
     6361 tcgttcaggg ttccagaact taagcctctc ctctttcata attggcctgt gcatgacgga
     6421 tataggctgg ctggtatgca gatttgccgt ttgccgcatc ttcccagcgt tgaagactca
     6481 agctggtaaa ccgcgtgcaa atgagcacgc cctctttcca tcaacactcc aggcagcttg
     6541 cgggaatgag tccactccgg tatcagaaat ggctacgtct caatgaaggc tacgtctcaa
     6601 tgaagtcagg cgaacgatgc tgaatgaaca ttacgatgtc accacagcag cctatgctgt
     6661 cggttacgaa agcttatccc atttcagccg ggaatatttg cggatgtttg gagagtcacc
     6721 taagagagat attaccaggt taagaaagtc tgtcggtcag ctttaataaa ccctgcactt
     6781 atctgtttag agcaattcgg tgttagttac accaggcaaa cattaactat agctaatgat
     6841 ttatagccat ataaaccatt agtagccctt gacctttcct gacacttccc ttgttcgcaa
     6901 taatattctg tttagtcacg gtttataaat aacatgcata atgaaatcgt catgttattt
     6961 agcgacataa aatgtttttg caaatactct cttcaacaag tgcagcaata aacttgttgt
     7021 gactaggtgt ccttgtgatt taaagatatt atcgttcttc gtgaaatgta ata
//
LOCUS       DAZUGK010000217         7066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZUGK010000217 DAZUGK010000000
VERSION     DAZUGK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.218"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            216..1091
                     /gene="rluB"
                     /locus_tag="ACHY5W_004055"
     CDS             216..1091
                     /gene="rluB"
                     /locus_tag="ACHY5W_004055"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFS4448832.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QMSGGRRSGGRNNNG"
     gene            complement(1131..1721)
                     /gene="cobO"
                     /locus_tag="ACHY5W_004056"
     CDS             complement(1131..1721)
                     /gene="cobO"
                     /locus_tag="ACHY5W_004056"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS4448833.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1718..2476)
                     /locus_tag="ACHY5W_004057"
     CDS             complement(1718..2476)
                     /locus_tag="ACHY5W_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFS4448834.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2696..3745
                     /gene="sohB"
                     /locus_tag="ACHY5W_004058"
     CDS             2696..3745
                     /gene="sohB"
                     /locus_tag="ACHY5W_004058"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFS4448835.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3781..4032)
                     /locus_tag="ACHY5W_004059"
     CDS             complement(3781..4032)
                     /locus_tag="ACHY5W_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFS4448836.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4412..7009
                     /gene="topA"
                     /locus_tag="ACHY5W_004060"
     CDS             4412..7009
                     /gene="topA"
                     /locus_tag="ACHY5W_004060"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4448837.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1799 a         1680 c         1916 g         1671 t
ORIGIN      
        1 taattaagct gatgttaatc gaaacaagct ttaaccataa agatagtgta gtatgttgcg
       61 cctcaaagcc aggccagtta aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac
      121 agactttact atcagtgagt cagatgtaag ttctgactca ctgttgcaca aaagcatatc
      181 agacgcctgg gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc
      241 tggcgcgtgc cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg
      301 tgagtgttga tggcaaaatt gctaaactcg gcgatcgtgt tgaagttacc cctggattga
      361 aaatccgtat cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg
      421 ttctggccta ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaaggacgtc
      481 caacggtgtt tgaccgtctg ccaaaactgc gtggcgcacg ctggattgcc gtgggtcgct
      541 tggacgttaa tacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt
      601 taatgcaccc aagccgtgaa gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg
      661 acgacgcgaa actgcgtgat ttgagtcgtg gcgtgcagtt ggaagatggt ccggcagctt
      721 ttaaaaccat caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga
      781 ccgaaggccg taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc
      841 gcctgatccg tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct
      901 ggacggagct ggacctcgcc cagactaact atctgcgcga actggtggag ctaccgccag
      961 aaaccagctc taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg aatcagattc
     1021 gtcgtgcggt gaaacgtcac agtcagatga gcggcggtcg tcgttctggc ggacgtaata
     1081 ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatcg
     1141 atccctatct gcgcttttac accggcatca aacgcatgtt tgatggggcg caactcactc
     1201 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
     1261 tgttgatgtg gacgttcatt taacgcctgc accacttcct ccagtggcaa atagtcatac
     1321 gccaccatat aagtcagctc atcaagcaaa accatatcca gtgaggaatc agcaagcatc
     1381 cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta
     1441 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg
     1501 cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga
     1561 ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
     1621 aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct
     1681 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
     1741 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
     1801 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
     1861 aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
     1921 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
     1981 catatgcgcc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
     2041 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
     2101 taacgttgac ctgtatgacg tcctgccaga cctgtggatt ttgttcgctc attggacaaa
     2161 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggcaa
     2221 tgcgctgtgc cagttgttgg caatcttcgg acctgcaggt cagcaaatcg agaataaacc
     2281 actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
     2341 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
     2401 gaccaatacc atcactggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
     2461 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtacgt
     2521 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
     2581 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
     2641 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
     2701 attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc
     2761 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
     2821 acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct
     2881 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc ataagcaaga
     2941 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaaccccg
     3001 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
     3061 agagataacg gctgtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
     3121 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
     3181 tgataaaaac attcctttaa ctgttacggt agacaaagtt gctgccagcg gcggttacat
     3241 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
     3301 ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
     3361 actgcacacc gccgggcagt ataagcgtac gctgaccttg ctgggtgaaa ataccgaaga
     3421 agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt
     3481 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
     3541 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
     3601 tagcctgatg gaaggccgtg aagtggtcaa tgtacgctat atgcagcgta aacgactcat
     3661 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgttac gctggtggca
     3721 gcgggggcaa aagccattga tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct
     3781 ttaatcaacc aggtgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
     3841 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
     3901 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
     3961 atgctcacga atgattttgt tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt
     4021 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
     4081 agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc
     4141 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
     4201 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
     4261 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca
     4321 gagaacgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
     4381 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
     4441 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg ttaaatccag
     4501 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
     4561 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
     4621 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga
     4681 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
     4741 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
     4801 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
     4861 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
     4921 tatggaccgc gtggtggggt atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg
     4981 cctgtctgcc ggtcgtgtgc agtcggtggc ggttcgtctg gtggtcgagc gtgagcgtga
     5041 aattaaagcg ttcgtgccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc
     5101 tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa
     5161 caaagaacaa actcaggcgg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
     5221 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
     5281 acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa accatgatga tggcgcagcg
     5341 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
     5401 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
     5461 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgccc
     5521 ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact
     5581 gtaccagtta atctggcgtc agttcgttgc ttgccagatg accccagcga aatatgactc
     5641 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
     5701 ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt
     5761 accggcagtc gataaaggcg atgctctgac gctcgttgaa ctgacaccag cccagcactt
     5821 taccaaaccg ccagctcgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgcgg
     5881 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggacc gtggctatgt
     5941 gcgtgtagaa aaccgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
     6001 ggaagagaat ttccgcgagt taatgaacta cgacttcacc gcgcagatgg aaaacagcct
     6061 tgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
     6121 tttcactcag cagttagata aagctgaaaa agatccggaa gaggggggta tgcgtccgaa
     6181 ccagatggtt ctgaccagca tcgactgccc gacctgtggt cgcaaaatgg gtattcgcac
     6241 agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg
     6301 caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
     6361 cgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
     6421 cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
     6481 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
     6541 gtgtgaaaaa tgtggttctg aaatgcacct gaaaatgggg cgtttcggta aatatatggc
     6601 ctgcaccaac gaagagtgta aaaacacgcg taagatttta cgtaacggcg aagtggctcc
     6661 accgaaagaa gatccggtac cattaccgga gctgccgtgc gaaaaatcag atgcctattt
     6721 cgtgctgcgt gacggtgctg ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg
     6781 tgaaacgcgt gcgccgctgg tggaagagct gtatcgcttc cgcgatcgtc tgccggaaaa
     6841 actgcgttat ctggccgatg cgccgcagca ggatccggaa ggtaataaga ctatggttcg
     6901 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
     6961 ctggtcagca ttttatgttg atggcaaatg ggttgaaggg aaaaaataac ctttaattct
     7021 gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttat
//
LOCUS       DAZUGK010000218         6988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZUGK010000218 DAZUGK010000000
VERSION     DAZUGK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.219"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            524..1513
                     /gene="araF"
                     /locus_tag="ACHY5W_004061"
     CDS             524..1513
                     /gene="araF"
                     /locus_tag="ACHY5W_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFS4448838.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1583..3097
                     /gene="araG"
                     /locus_tag="ACHY5W_004062"
     CDS             1583..3097
                     /gene="araG"
                     /locus_tag="ACHY5W_004062"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFS4448839.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3112..4098
                     /gene="araH"
                     /locus_tag="ACHY5W_004063"
     CDS             3112..4098
                     /gene="araH"
                     /locus_tag="ACHY5W_004063"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFS4448840.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4265..5065
                     /gene="otsB"
                     /locus_tag="ACHY5W_004064"
     CDS             4265..5065
                     /gene="otsB"
                     /locus_tag="ACHY5W_004064"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFS4448841.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5040..6464
                     /gene="otsA"
                     /locus_tag="ACHY5W_004065"
     CDS             5040..6464
                     /gene="otsA"
                     /locus_tag="ACHY5W_004065"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFS4448842.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6471..6899)
                     /gene="uspC"
                     /locus_tag="ACHY5W_004066"
     CDS             complement(6471..6899)
                     /gene="uspC"
                     /locus_tag="ACHY5W_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFS4448843.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1763 a         1632 c         1816 g         1777 t
ORIGIN      
        1 atttgaattc atgatgaagt gatgatttta taaaacgttt tttcattttt gcgagatggc
       61 tctcattata cgtgtcctgt taattaatga gcacagtgat aattaatggc gaaatgaatg
      121 gcgcgcattt tccacatctt tgagttgtgg ttattacacc atttcaaaaa ctcaacgcca
      181 ggtaatgcgg cctattgact ggttaaaaag aagacatccc gcatgggtac caaagacaac
      241 aaggatttcc aggctaatct tatggattaa tctgctgtgc attcgacaat ttgtctgaca
      301 aattggcttt cctttatgtc ttttcccgct aaatttatgc acgttctcac tgtaattctg
      361 cgatgtgata ttgctctcct atggagaatt aatttctcgc taaaactatg tcaacacagt
      421 cacttatctt ttagttaaaa ggtaatgctt tgttttccga ttaatttaac gaatgtcatt
      481 cgtttttgcc ctacacaaaa cgacactaaa gctggagaga accatgcaca aatttactaa
      541 agccctggca gccattggtc tggcagccgt tatgtcacaa tccgctatgg cggagaacct
      601 gaagctcggt ttcctggtga agcaaccgga agagccatgg ttccagaccg aatggaagtt
      661 tgccgataaa gctgggaagg atttagggtt tgaggttatt aagattgccg tgccggatgg
      721 cgaaaaaaca ttgaacgcga tcgacagcct ggcagccagt ggcgcgaaag gtttcgttat
      781 ttgtactccg gacccgaaac tcggctctgc catcgtggcg aaagcgcgtg gctacgatat
      841 gaaagtcatt gccgtggatg accagtttgt taacgccaaa ggtaagccaa tggataccgt
      901 tccgttggtg atgatggcgg cgaccaaaat tggcgaacgt cagggccagg aactgtataa
      961 agagatgcag aaacggggct gggatgtcaa agaaagcgcg gtgatggcga ttaccgccaa
     1021 cgaactggat acggctcgtc gccgtactac ggggtctatg gatgcgctga aagctgcagg
     1081 attcccggaa aaacaaattt atcaggtacc taccaaatct aacgacatcc cgggggcatt
     1141 tgacgctgcc aactcaatgc tggttcaaca tccggaagtt aaacattggc tgatcgtcgg
     1201 tatgaacgac agcaccgtac ttggcggcgt acgtgcaacc gaaggtcagg gctttaaagc
     1261 agccgatatc atcggtattg gtattaacgg tgtggatgcc gtaagcgaac tgtctaaagc
     1321 acaggcaacc ggcttctacg gttccctgct gccaagcccg gatgttcatg gctataaatc
     1381 cagcgaaatg ctttacaact gggtagcaaa agacgttgaa ccgccaaaat ttaccgaagt
     1441 taccgacgtg gtactgatca cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt
     1501 aggcggtaag taatttgccg gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg
     1561 accagtgatt cacggagacg ttatgcaaca gtctaccccg tatctctcat ttcgcggcat
     1621 cggtaaaacg tttcccggcg ttaaggcgct gacggatatt agttttgact gctatgccgg
     1681 tcaggttcat gcgttgatgg gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct
     1741 cagcggcaac tatgcgccaa ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt
     1801 ttccgacacg accgcagcac ttaacgcggg tgtggcgatt atttaccagg aactgcatct
     1861 cgtgccggaa atgaccgtcg cggaaaacat ctatctcggc cagctgccgc ataaaggcgg
     1921 cattgtgaat cgctcattac tgaattatga ggcgggttta caacttaaac atcttggtat
     1981 ggatattgac ccggacacgc cgctgaaata tctctccatt ggtcagtggc agatggttga
     2041 aattgccaag gcgctggcgc gtaacgctaa aattatcgcc tttgatgagc caaccagctc
     2101 cctttctgcc cgcgaaatcg acaatctttt ccgcgttatt cgtgaactgc gaaaagaggg
     2161 gcgggtgatc ttatacgttt ctcaccgtat ggaagaaata tttgccctca gcgatgccat
     2221 cactgtcttt aaagatggac gttatgtcaa aacctttacc gatatgcagc aggttgacca
     2281 cgacgcgctg gtgcaggcga tggtcgggcg cgacattggc gatatctacg gctggcaacc
     2341 gcgtagttat ggcgaggagc gcctgcgtct tgatgctgtg aaagcaccag gcgtgcgtac
     2401 gccaataagt ctggcggttc gcagtggtga aattgttggg ctgtttggtc tggtaggggc
     2461 ggggcgtagc gaattaatga aaggcatgtt tggcgggacg caaatcaccg ccggtcaggt
     2521 ttatatcgac caacagccga tcgatattcg taaaccgagc cacgccattg ccgcaggcat
     2581 gatgctctgc ccggaagatc gcaaagcgga aggcattatt cccgtgcact ccgttcgcga
     2641 caatatcaac atcagtgcca gacgtaaaca tgtgctcggc ggttgtgtaa tcaacaacgg
     2701 ttgggaagaa aacaatgccg atcaccacat tcgttcgctc aacatcaaaa cgccgggcgc
     2761 ggagcaactg atcatgaatc tctcaggcgg aaatcagcaa aaagccattc tgggccgctg
     2821 gttatcggaa gagatgaagg tcattttgct ggatgaacct acgcgcggca tcgatgttgg
     2881 cgctaagcac gaaatatata acgtaattta tgcgctggcg gcgcagggcg tggcggtgct
     2941 gtttgcctcc agcgacttac ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg
     3001 ggaaggtgaa atcgccggtg aattgttaca cgagcaggca gatgagcgtc aggcactgag
     3061 ccttgcgatg cctaaagtca gccaggctgt tgcctgagta aggagagtat gatgtcttct
     3121 gtttctacat cggggtctgg cgcacctaag tcgtcattca gcttcgggcg tatctgggat
     3181 cagtacggca tgctggtggt gtttgcggtg ctctttatcg cctgtgccat ttttgtccca
     3241 aattttgcca ccttcattaa tatgaaaggg ttgggcctgg caatttccat gtcggggatg
     3301 gtggcgtgtg gcatgttgtt ctgccttgct tccggtgact ttgacctttc tgtcgcctcc
     3361 gtaattgcct gtgcgggtgt caccacggcg gtggttatca acctgactga aagcctgtgg
     3421 attggcgtgg cagcggggtt gctgcttggc attctctgtg gcctggtcaa tggctttgtt
     3481 atcgccaaac tgaaaataaa tgctctgatc acgacactgg caacgatgca gattgttcga
     3541 ggtctggcgt acatcatttc cgacggtaaa gcggtcggta tcgaagatga aagcttcttt
     3601 gcccttggtt acgccaactg gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt
     3661 ctgattatct ttggtttgct gctgaataaa actacctttg gtcgtaatac cctggcgatt
     3721 ggcgggaacg aagaggctgc gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt
     3781 atctttgttc tctctggcct ggtatctgcg atagccggaa ttattctggc ttcacgtatg
     3841 accagtgggc agccaatgac gtcgattggt tatgagctta ttgttatctc cgcctgcgtt
     3901 ttaggtggcg tttctctgaa aggtggcatc ggaaaaatct catatgtggt ggcgggtatc
     3961 ttaattttag gcaccgtgga aaacgccatg aacctgctta atatttctcc tttcgcgcag
     4021 tacgtggttc gcggcttaat cctgctggca gcggtgatct tcgaccgtta caagcaaaaa
     4081 gcgaaacgca ctgtctgatg cttttttcca caacaattta acgttttttc ccaccacagc
     4141 cagccgccac aacggttggc tgttcttcgt tgcaaatggc gacccccgtc acactgtcta
     4201 tacttacatg tctgtaaagc gcgttctgcg caacacaata agaaaagaga aggaggagaa
     4261 ccgggtgaca gaaccgttaa ccgaaacccc tgaactatcc gcgaaatatg cctggttttt
     4321 tgatctcgat ggaacgctgg cggaaatcaa accgcatccc gatcaggtcg tcgtacctga
     4381 caatattctg caaggacttc agctactggc aaccgcaagt gatggtgcat tggcattgat
     4441 atcagggcgc tcaatggtgg agctggacgc actggcaaaa ccttatcgct tcccgttagc
     4501 gggcgtgcat ggggcggagc gccgtgacat caatggtaaa acgcatatcg ttcatctgcc
     4561 ggatgcgatt gcacgtgata ttaacgtgca actgcatacg gtcatcgccc agtatcccgg
     4621 tgcggagctg gagacgaaag ggatggcttt tgcgctgcat tatcgtcagg ctccgcagca
     4681 tgaagacgca ttaatgacat tagcgcaacg tattactcag atctggccac aaatggcgtt
     4741 acagcaggga aagtgtgttg tcgagatcaa accgagaggt accagtaaag gtgaggcgat
     4801 tgctgccttt atgcaggaag ctccctttat cgggcgaacg cccgtatttc tgggcgatga
     4861 tttaaccgat gaatctggct tcgcagtcgt taaccgactg ggcggaatgt cagtaaaaat
     4921 tggcacaggc gcaactcagg catcatggcg actggcgggc gtaccggatg tctggagctg
     4981 gcttgaaatg ataaccaccg cattacaaca aaaaagagaa aataacagga gtgatgacta
     5041 tgagtcgttt agtcgtagta tctaaccgga ttgcaccacc agatgagcac gccgccagtg
     5101 ccggtggcct tgccgttggc atactggggg cactgaaagc cgcaggcgga ctgtggtttg
     5161 gctggagtgg tgaaacaggg aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca
     5221 ttacgtgggc ctcttttaac ctcagcgaac aggaccttga cgaatactac aaccaattct
     5281 ccaatgccgt tctctggccc gcttttcatt atcggctcga tctggtgcaa tttcagcgtc
     5341 ctgcctggga cggctattta cgcgtaaatg cgttgctggc agataaatta ctgccgctgt
     5401 tgcaagacga tgacattatc tggatccacg attatcacct gttgccattt gcgcatgaat
     5461 tacgcaaacg gggagtgaat aatcgcattg gtttctttct gcatattcct ttcccgacac
     5521 cggaaatctt caacgcgctg ccgacatatg acaccttgct tgaacagctt tgtgattatg
     5581 atttgctggg tttccagaca gaaaacgatc gtctggcgtt cctggattgc ctttctaacc
     5641 tgacccgcgt cacgacacgt agcgcaaaaa gccatacagc ctggggcaaa gcgtttcgaa
     5701 cagaagttta cccgattggc attgagccaa aagaaatagc caaacaggct gccgggccac
     5761 tgccgccaaa actggcgcaa cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg
     5821 tcgaacggct ggattattcc aaaggtttgc cagagcgttt tctcgcctat gaagcgttgc
     5881 tggaaaaata tccgcagcat catggtaaaa ttcgttatac ccagattgca ccaacgtcgc
     5941 gtggtgatgt gcaagcctat caggatattc gtcatcagct cgaaaatgaa gctggacgaa
     6001 ttaatggtaa atacgggcaa ttaggctgga cgccgcttta ttatttgaat cagcattttg
     6061 accgtaaatt actgatgaaa atattccgct actctgacgt gggcttagtg acgccactgc
     6121 gtgacgggat gaacctggtg gcaaaagagt atgttgccgc tcaggaccca gccaatccgg
     6181 gcgttcttgt tctttcgcaa tttgcgggag cggcaaacga gttaacgtcg gcgttaattg
     6241 ttaatcccta cgatcgtgac gaagttgcag ctgcgctgga tcgtgcattg actatgtcgc
     6301 tggcggaacg tatttcccgt catgcagaaa tgctggacgt tatcgtgaaa aacgatatta
     6361 accactggca ggagtgcttc attagcgacc taaagcagat agttccgcga agcgcggaaa
     6421 gccagcagcg cgataaagtt gctacctttc ctaagcttgc gtaggagcgg ttaatctccc
     6481 gtaagtggaa ctaacagcac atcgacctca cttgaggcaa tcactctttt cgccgagcag
     6541 gatgctcgcg aaaagaaact gtgattgtga ttaccgcaaa tcaccaaatc gaaatgatat
     6601 ttgcgacaaa cttccagaat atgttcgctt aactcaccgt aggcaataaa cgtcttgtca
     6661 acgggatacc ccgcatcctg aattaactta tcaagaaagc tttgcgtttc ttcctgcatc
     6721 acgctgcgta aatcttcaag catcggcgca gctaattgat tgtacatttc cgggtcggaa
     6781 gcgagagtaa ttaaactgat gtgtcccttt actggcctgg cgatagatac cgcttttgcc
     6841 agcagttgct gactttccgg tgtaaccgca acagcgacaa gaatattgct atagctcata
     6901 acctgttcct tattctgtga acttcaggtg acattaaagc tttactccta atggagaaac
     6961 gacgcaatcc caactcagtc aaacaggt
//
LOCUS       DAZUGK010000219         6977 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZUGK010000219 DAZUGK010000000
VERSION     DAZUGK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.220"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(118..561)
                     /locus_tag="ACHY5W_004067"
     CDS             complement(118..561)
                     /locus_tag="ACHY5W_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4448844.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            718..1647
                     /gene="metA"
                     /locus_tag="ACHY5W_004068"
     CDS             718..1647
                     /gene="metA"
                     /locus_tag="ACHY5W_004068"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFS4448845.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDARESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1916..3517
                     /gene="aceB"
                     /locus_tag="ACHY5W_004069"
     CDS             1916..3517
                     /gene="aceB"
                     /locus_tag="ACHY5W_004069"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFS4448846.1"
                     /translation="MTEQATTTDELAFTRLYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEVGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3547..4851
                     /gene="aceA"
                     /locus_tag="ACHY5W_004070"
     CDS             3547..4851
                     /gene="aceA"
                     /locus_tag="ACHY5W_004070"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFS4448847.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAKGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            4937..6673
                     /gene="aceK"
                     /locus_tag="ACHY5W_004071"
     CDS             4937..6673
                     /gene="aceK"
                     /locus_tag="ACHY5W_004071"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFS4448848.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAAQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTENLSESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNCIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(6642..>6977)
                     /locus_tag="ACHY5W_004072"
     CDS             complement(6642..>6977)
                     /locus_tag="ACHY5W_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448849.1"
                     /translation="NNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMD
                     FWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALL
                     KKASPHTVR"
BASE COUNT         1723 a         1797 c         1773 g         1684 t
ORIGIN      
        1 aagcgaaagg ccatccttca ggatggcctt ttttgcgttg gtgcaaaaaa atgccggatg
       61 cgacgctggc gcgtcttatc cagcttacgc aggcacgata gcacgatagg gggcagctta
      121 ttcccccaca tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga
      181 gcgtcccgta accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc
      241 attaacgttg gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc
      301 cacgccgcag ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttag
      361 caacataaat ccaaccggct gatcccgttc attaaccgcg acccacaacg gtgcttccgg
      421 cagaaaggaa cgaaccagct cttccagctc ggcccgatac tctgctgata aaaaatcgtg
      481 agtggcatcg acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga
      541 gcggcgaata ctaataacca ttttctctcc ttttagtcat tcttatattc taacgtagtc
      601 ttttccttga aattttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt
      661 atcttcagct atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg
      721 ccgattcgtg tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt
      781 gtgatgacaa cttctcgcgc gtctggtcag gaaattcgtc cgctaaaggt gcttatcctt
      841 aacctgatgc cgaagaagat cgaaactgaa aatcagtttc ttcgcctgct ttcaaactca
      901 cctttgcagg tcgatattca gctgttgcgc atcgatgccc gtgaatcgcg caacacgccc
      961 gcagagcatc tgaataactt ctactgtaac tttgaagata ttcaggaaca aaactttgac
     1021 ggtctgatag ttaccggcgc gccgctgggc ctggtggagt ttaatgatgt tgcttactgg
     1081 ccgcagataa aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc
     1141 tgctgggcgg tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc
     1201 gacaaactct ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt
     1261 ggctttgatg attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg
     1321 attcgtgatt acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg
     1381 tttgccagca aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa
     1441 acgctggcgc aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat
     1501 aactatttcc cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt
     1561 aatttgctgt ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta
     1621 cggcacatga atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag
     1681 caccttacct caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaggtg
     1741 aataaatttt attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa
     1801 ttgtttttga ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca
     1861 acgatccttc gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac
     1921 tgaacaggca acaacaaccg atgaactggc tttcacaagg ctgtatggcg agcaggagaa
     1981 gcaaattctt actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc
     2041 acaacgcaat aaacttctgg cggcgcgtat tcagcagcag caagatattg ataacggaac
     2101 gttgcctgat tttatttcgg aaacagcttc cattcgtgat gctgactgga aaattcgcgg
     2161 gattcctgcg gacttagaag atcgccgcgt agagattacc ggacccgtag agcgcaagat
     2221 ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact
     2281 ggcaccagac tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cggttaacgg
     2341 caccatcagt tacaccaatg aagtaggcaa aatttaccag ctcaagccca atccagcggt
     2401 tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa catgtcacct ggcgtggtga
     2461 ggcaatcccc ggcagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact
     2521 gttggcaaag ggcagtggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc
     2581 ggcctggtgg agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcac
     2641 catcaaggcg acgttgctga ttgaaacgtt gcctgccgtg ttccagatgg acgaaatcct
     2701 tcacgcgctg cgtgaccata ttgttggtct gaactgcggt cgttgggatt acatcttcag
     2761 ctatatcaaa acgttgaaaa actatcccga tcgcgtcctg ccagacagac aggcagtgac
     2821 gatggataaa ccattcctga atgcttactc acgcctgttg attaaaacct gccataaacg
     2881 cggtgctttt gcgatgggcg gcatggcggc gtttattccg agcaaagatg aagagcgcaa
     2941 taaccaggtg ctcaacaaag taaaagcgga taaatcgctg gaagccaata acggtcacga
     3001 tggcacatgg atcgctcacc caggccttgc ggatacggca atggcggtat tcaacgacat
     3061 tctcggctcc cgtaaaaatc agcttgaagt gatgcgcgaa caagacgcgc cgattactgc
     3121 cgatcagcta ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgcaaatat
     3181 tcgcgtagcc gtgcagtaca tcgaagcatg gatctccggc aacggctgcg tgccaattta
     3241 tggcctgatg gaagatgcgg cgacggctga aatttcccgt acctcaatct ggcagtggat
     3301 ccatcatcaa aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgccagat
     3361 gctgggcgaa gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg
     3421 gcgttttgac gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga
     3481 tttcctgacc ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat
     3541 ctgcacatga aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg
     3601 cgttgggaag gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca
     3661 gtcaatcctg aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac
     3721 ggtgaatcga aaaaaggcta catcaacagc ctcggcgcac tgactggcgg tcaggcgctg
     3781 caacaggcga aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac
     3841 gctaacctgg cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca
     3901 gctgtggtgg agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg
     3961 ggcattgagc cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg
     4021 gaagccggtt ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc
     4081 ggtgcagcgg cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg
     4141 ggcggcaaag ttttagtgcc aactcaggaa gctattcaga aactggtcgc ggcgcgtctg
     4201 gcagctgacg tgacgggcgt tccaaccctg ctggttgccc gtaccgatgc tgatgcggcg
     4261 gatctgatca cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc
     4321 agtgaaggct tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg
     4381 tatgcgccat atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg
     4441 cgtcgctttg cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc
     4501 tcgccgtcgt tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag
     4561 cagctgtcgg atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg
     4621 tggttcaaca tgtttgacct ggcaaacgcc tatgccaagg gcgagggtat gaagcactac
     4681 gttgagaaag tgcagcagcc ggaatttgcc gccgcgaaag atggctatac cttcgtatct
     4741 caccagcagg aagtgggtac aggttacttc gataaagtga cgactattat tcagggcggc
     4801 acgtcttcag tcaccgcgct gaccggctcc actgaagaat cgcagttcta agcaacctac
     4861 aaacgttgct gactgtaggc cggataaggc atttacgccg catccggcaa ttctctgctc
     4921 ctgatgaggg cgctaaatgc cgcgtggcct ggaattactg attgctcaaa ccattttgca
     4981 aggcttcgac gctcagtatg gtcgattcct cgaagtgact tccggggcgc agcagcgttt
     5041 cgaacaggcc gactggcatg ctgcccagca ggcgatgaaa aaccgtatcc atctttacga
     5101 tcatcacgtt ggtctggtcg tggagcaact gcgctgcatt actaacggcc aaagtacgga
     5161 cgcggcattt ttactgcgcg ttaaagagca ttacacccgg ctgttgccgg attacccgcg
     5221 cttcgagatt gcggagagct tttttaactc cgtgtactgt cggttatttg accaccgctc
     5281 gcttactccc gagcggcttt ttatctttag ctcccagcca gagcgccgct ttcgtaccat
     5341 tccccgcccg ctggcgaaag acttttaccc cgatcacggc tgggaatctc tactgatgcg
     5401 cgttatcagc gacctgccgc tgcgcctgcg ctggcagaat aaaagccgtg atatccatta
     5461 cattattcgc catctgacgg aaacgctggg gacagaaaac ctctccgaaa gccatttaca
     5521 ggtggcgaac gaactgtttt accgcaataa agccgcctgg ctggtgggca aactgatcac
     5581 gccttccggc acgttgccat ttttgctgcc gatccaccag acggacgacg gcgagttatt
     5641 tattgatact tgcctgacga cgaccgccga agcgagcatt gtttttggct ttgcgcgttc
     5701 ttattttatg gtttatgcac cgctgcccgc agcgctggtc gagtggttac gggaaattct
     5761 gccaggtaaa accaccgctg aattgtatat ggctatcggc tgccagaagc acgccaaaac
     5821 cgaaagctac cgcgaatatc tcgtttatct acagggctgt aatgagcagt tcattgaagc
     5881 gccgggtatt cgtggcatgg tgatgttggt gttcacgctg ccgggctttg accgtgtatt
     5941 taaagtcatc aaagacaagt tcgcgccgca gaaagagatg tctgccgctc acgttcgtgc
     6001 ctgctatcaa ctggtgaaag agcacgatcg cgtgggccga atggcggaca cccaggagtt
     6061 tgaaaacttt gtgctggaga agcggcatat ttccccggca ttaatggaat tactgctcca
     6121 ggaagcagcg gaaaaaatca ccgatctcgg cgaacaaatt gtgattcgcc atctttatat
     6181 tgagcggcgg atggtgccgc tcaatatctg gctggaacaa gtggaaggtc agcagttgcg
     6241 cgacgccatt gaagagtacg gtaacgctat tcgccagctt gccgctgcta acattttccc
     6301 tggcgacatg ctgtttaaaa acttcggtgt cacccgtcac gggcgtgtgg ttttttatga
     6361 ttacgatgaa atttgctaca tgacggaagt gaatttccgc gacatcccgc cgccgcgcta
     6421 tccggaagac gaacttgcca gcgaaccgtg gtacagcgtc tcgccgggcg atgttttccc
     6481 ggaagagttt cgccactggc tatgcgccga cccgcgtatt ggtccgctgt ttgaagagat
     6541 gcacgccgac ctgttccgcg ctgattactg gcgcgcacta caaaactgca tacgtgaagg
     6601 gcatgtggaa gatgtttatg cgtatcggcg caggcaaaga tttagcgtac ggtatgggga
     6661 gatgcttttt tgagtaaagc ttccatataa tttttctccg caatgtatcg agggttatcc
     6721 gtaaagccaa agctttcagc catcttattt aatgtattaa ggattaattc agcaataacc
     6781 cggtgaccca attcaaaagc caactcaaag gcagagtatt tttgtggcgc tttgtgttgc
     6841 caaaaatcca taatatcttc agcagtaaat ccaaacaggc gtgcatggtc agataaagca
     6901 agataaaccg tctctacaac gttttgttgt ttatgctgta tcgctgaaaa caaaccggga
     6961 tattcattag aattatt
//
LOCUS       DAZUGK010000220         6967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZUGK010000220 DAZUGK010000000
VERSION     DAZUGK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.221"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            57..1835
                     /locus_tag="ACHY5W_004073"
     CDS             57..1835
                     /locus_tag="ACHY5W_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4448850.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1875..2354)
                     /gene="xdhC"
                     /locus_tag="ACHY5W_004074"
     CDS             complement(1875..2354)
                     /gene="xdhC"
                     /locus_tag="ACHY5W_004074"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFS4448851.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2351..3229)
                     /gene="xdhB"
                     /locus_tag="ACHY5W_004075"
     CDS             complement(2351..3229)
                     /gene="xdhB"
                     /locus_tag="ACHY5W_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFS4448852.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3240..5537)
                     /gene="xdhA"
                     /locus_tag="ACHY5W_004076"
     CDS             complement(3240..5537)
                     /gene="xdhA"
                     /locus_tag="ACHY5W_004076"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFS4448853.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5953..6708
                     /gene="actS"
                     /locus_tag="ACHY5W_004077"
     CDS             5953..6708
                     /gene="actS"
                     /locus_tag="ACHY5W_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFS4448854.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6787..6860
                     /locus_tag="ACHY5W_004078"
     tRNA            6787..6860
                     /locus_tag="ACHY5W_004078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6819..6821,aa:Gly,seq:ccc)
BASE COUNT         1732 a         1679 c         1786 g         1770 t
ORIGIN      
        1 gtgaaccatc aacgtcttca atgatcgact tttaagtaag tctggaaggt aaagctatgg
       61 agcttgctac tacgcagtca gtattgatgc aaattcaacc gacaattcag cgttttgcca
      121 gaatgcttgc cagcgttttg cagcttgagg ttgagatcgt tgatgaaaac ttgtgtcgcg
      181 tcgccggaac gggcgcgtat gggaagtttc ttggtcgcca gttgagcggc aactcacgcc
      241 tgctccgcca cgtcctggaa acgaaaactg aaaaagttgt gacacagtct cgcttcgatc
      301 ccctttgcga aggttgcgat agtaaagaaa attgccgcga aaaagcattt ctgggtacgc
      361 ctgtcatttt acaggatcgt tgtgttgggg tgataagttt gattgccgtt acccacgagc
      421 aacaagagca tatcagtgat aatttacgcg aattttctga ttatgttcgt catatatcca
      481 ccatttttgt ttcgaaactt ctggaggatc aggggccagg agataacatc agtaaaatat
      541 tcgcgaccat gatcgataat atggatcagg gcgtattagt tgtagatgat gaaaatcggg
      601 ttcagtttgt taatcagact gccttaaaaa cacttggtgt tgtgcaaaat aatattattg
      661 ggaaacctat ccgtttcaga ccattaacat ttgagagtaa ttttacccat ggacatatgc
      721 agcatattgt ttcgtgggac gataaaagtg aattaatcat tggtcaattg cataacattc
      781 agggccgaca attattttta atggcgtttc accaatcgca taccagtttt tctgtagcaa
      841 atgcacctga tgaaccgcat attgaacaat tggttggcga gtgccgtgtt atgcggcaat
      901 taaaacgact cattagccgt attgcaccca gcccatccag cgttatggtg gttggtgaaa
      961 gcggcacggg taaagaagtt gtcgcccgtg caatccataa gttgagcgga agacggaata
     1021 aaccctttat tgctatcaac tgtgccgcga ttccggagca gcttctggag agcgaactgt
     1081 tcggttatgt taaaggcgca tttactggcg cttctgccaa cggtaaaaca gggttgattc
     1141 aggcggcgaa tacgggcacg ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc
     1201 aggctaaatt actgcgcgct attgaggcac gtgaaattct gccgattggt gccagtagcc
     1261 caatacaagt cgacattcgc atcatttctg caactaacca gaatttggcc cagttcattg
     1321 ccgaaggtaa attccgcgaa gatctcttct accgacttaa tgttatcccg ataactctgc
     1381 caccgctgcg tgaacgtcag gaagatattg aactactggt gcattacttt ttacatctgc
     1441 atacccgtcg tctgggatcg gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc
     1501 gtaagcatcg ttggcccgga aacctgcgcg agttaagcaa tttgatggaa tatctggtta
     1561 acgttgttcc ttcaggtgaa gttatcgaca gcacgctatt gccgccaaat ctgctgaata
     1621 atggcacaac ggagcaaagt gatgtaacag aggtcactga ggcgcacctg tcactcgatg
     1681 atgcgggcgg cacggcgctg gaggagatgg aaaagcaaat gatccgcgag gcgctttcac
     1741 gtcataacag caagaagcaa gttgctgatg aactgggcat cggcattgct acgctctatc
     1801 gcaagattaa gaaatatgag ttgttaaaca cataacctca gcgaatccgg cctgaattca
     1861 ggccggatat ccttttactt cgttttctcg caatccagaa ctgtatttac aatcatctga
     1921 taccccgtgc agcgacaaag atttcccgcc agtccgcgac gaatttccgt aatggttaat
     1981 ggcttctcgc gtggtttcgc cagcattgcc gtggtagcca taatcaggcc aggcgtacaa
     2041 aacccgcact gcactgcgcc ggatttcgca taagcctgct gaacatgaga aagttttccg
     2101 cctttcgctt caccttcaag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat
     2161 aagcaactgt ctattgctgt gccgtcgacc agcaccgtac aggcaccaca ttcacccacg
     2221 cagcaccctt gtttgacact tagcagtcct tgttcgcgga gtaattccga gagcggcgtg
     2281 cctggtgcgg catgaagctg aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt
     2341 tcgctgtgat tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt
     2401 tggtcatcgt ctggatgaga tgcagacgaa actctttact ggcccgccat gaagaacgcg
     2461 gggcgacatc ttgcaggaca gattcgctga tagcttccag cgtttgcagg tttaatggcg
     2521 cattttgtgc agtctgttcg gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa
     2581 atgccaggcg taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcacagccaa
     2641 tcgttgaaat atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccacgtgtt
     2701 ctttcggctg gggtggaaaa tgaaaggcga caaggatttc gtcatgctca agagacactt
     2761 tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg aacactgcgt ggggagtgga
     2821 tctccagttt cgcgtcataa atgagcgttg gcgtggcaga atctgcgctg gtggcaccgt
     2881 tgcaaatatt tccaccgtag gtagcgacgt tacggatctg cggtccagca atggacgagg
     2941 ccgcagcaca taacgccggg agatgacgtt gagttatagg atcttctatt agctgggtaa
     3001 atgtcgttgc agagccgata cgtagcgagc catcttccgc cagcgtaatt ccccgcagct
     3061 ccgccagatt atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa
     3121 tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga
     3181 tggcatcggc aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct
     3241 caaatcaatc ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta
     3301 ttgattgcaa caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt
     3361 attggtggct cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg
     3421 aacgcgcttt ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg
     3481 gggttacgga ccacgccgct ttttgcatcg atgatcattt cttcaaatag cgcccagcca
     3541 atgcccattc ccattccgcc gtgtacctga ccttctgcca gtagcggatt aagaatatgg
     3601 cccgaatcat gaacgttgag gatgcggttg atggtgactt tgcacagcgc aatatcgacc
     3661 gtcagatcaa caaaggtaca gccgaacgcc ggtgggttag tggtggtttt gatggagctt
     3721 tcagcagaga gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt
     3781 aacgacatta acggctcttc cggtcgctca accagcacga tatggccttt tatcagggtc
     3841 agattcatcg ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat
     3901 aacagtgctg cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg
     3961 ccgggatcga acggcgtaac atcggtatct tgtgttgaaa taacgcgaac gtcgctgacc
     4021 ggaaccccca cggtttctgc caccatttgc gagaagacgg tatcggcacc ctgaccgatt
     4081 tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct
     4141 gctatttcta cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg
     4201 ccgcggcgta aattgccttg ctggttctgg cactctgcac ggcgtttttc ccattcaaag
     4261 attttccggc ctttttcaag acactccggc aaccctgcgc tgtaaatacg tttgcccgtg
     4321 agcggattag catctccttc gcgtgcggcg ttgcgtaaac gaatttcaac aggatcaata
     4381 cctaacgctg tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg
     4441 ccataaccac gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa
     4501 gcgtaggcac aacgaggata aaggtaagcg actttattcc cccccgcaga agcgatggag
     4561 tgcccgtgag atgcataagc gccggtgtta gacagaacat ccagactata acctttcaat
     4621 gttccgtcgc ggttcacgcc catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt
     4681 gcgaggaaac actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg
     4741 gtcaggaatg ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg
     4801 acaaatggtt tgatgactcg tacgcatgac cagggaatat ccagtgcctg accaaccacg
     4861 cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc
     4921 atccacgcca gcgatgttac gctttccatg tgacaatgtt gaataacggg ggtctgatag
     4981 tgcccctgta cctggtagtc ggcggcatcg attgtttgtt ggacattgcc cgtcgacatc
     5041 gtgctttgtt tcagtaaatt gccgccgtta tggattggtg cagcgtcctc tgccagcgcc
     5101 gcttctggcg tggtgataac gggtaattct tgccactcaa tgctgaccaa ttgcgccgct
     5161 ttttctgccg tgagttcatc gcgggccacg acgatggcaa cggcgtcacc atgatgacga
     5221 acatggcgag ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccat
     5281 gcatgcccag ctgtagcgaa tgggatatca ggcacatctt cccaggtaaa aatcgccagt
     5341 acgcctggta aacttctggc ttgttcatca ttaatactta cggcataacc atgtgcgata
     5401 gggctacgta catatttcgc ataacacatg cccgccataa cataatcgtc agtatatcgt
     5461 gcccgcccgg tgaccttagc aatggcatcg acgcgcatgc atgattcacc cgtagcggtt
     5521 gcttcccgcg cttccataga aatcccctcg atttgaacat ttggttctta tggcaaagat
     5581 ttagtctaag caggcaagat ttacgccaga aacgacgata agcgttaggg gaggaaggaa
     5641 gtgtgatgac gttaaaaaaa cacagatatg atacgaaaca ggactctgaa taggggcagt
     5701 gatacgtatc aatatgagtg gcgataaaat caatgtgata aattgggatt attacgcatc
     5761 aggatgggaa atagggtaaa aaagtcagga aattcgatac ctctacagtg aatattttgc
     5821 tgatgatact tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca
     5881 gaagcaagag attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag
     5941 cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg
     6001 ttatcggttg gactgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg
     6061 tattccggct ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc
     6121 acgggaacca gcgtaaaaga gctggcgcga ctgaacggca tttccccccc ttacaccatt
     6181 gaagttggtc agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca
     6241 accgccaaat caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct
     6301 tcctggccgc cagtaggaca acgttgttgg ttatggccaa cgacagggaa agttatcatg
     6361 ccgtattcga cagcagatgg cggcaataaa gggattgata tctctgctcc acggggtaca
     6421 cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac
     6481 ggtaatctca tcatgattaa acacagtgaa gattacatta cggcttacgc ccataatgac
     6541 acgatgctgg taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc caccatgggg
     6601 agcacggatg cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt
     6661 gatccgctac gttacttgcc gccgcagggc agcaagccaa aatgctgatg gcgaattaat
     6721 cagcagtcag cagcgcggca cttgctaagg agagcgtaag gtttataatg ccttacgcat
     6781 ctcgaagcgg gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc
     6841 gattcccttc gcccgctcca atttatcttc acctcatcaa tctgccattt ttgctgtatc
     6901 tgattttcta tattatctta attcaatcgc ttcagacttt tcccaatcgc tgtaacactt
     6961 ccccatt
//
LOCUS       DAZUGK010000221         6916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZUGK010000221 DAZUGK010000000
VERSION     DAZUGK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.222"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..517
                     /locus_tag="ACHY5W_004079"
     CDS             <1..517
                     /locus_tag="ACHY5W_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4448855.1"
                     /translation="SEQEVLSLQAAPHVFLDGLSEPIGKFPMTDSWYPSRDFRRRAAL
                     WGMALPETEFTPAELTAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVNH
                     AAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRTF
                     EALDCTDWRDD"
     gene            510..971
                     /locus_tag="ACHY5W_004080"
     CDS             510..971
                     /locus_tag="ACHY5W_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS4448856.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYNGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPLGPTPIELLKQEYERRKAAGFV"
     gene            1006..1767
                     /locus_tag="ACHY5W_004081"
     CDS             1006..1767
                     /locus_tag="ACHY5W_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4448857.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIAQEGAKTCEEI
                     AGKFSVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTENDGKA
                     FPQPACVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            1764..1943
                     /locus_tag="ACHY5W_004082"
                     /pseudo
     CDS             1764..1943
                     /locus_tag="ACHY5W_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            2364..2915
                     /locus_tag="ACHY5W_004083"
     CDS             2364..2915
                     /locus_tag="ACHY5W_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS4448858.1"
                     /translation="MTTQVTVKNLSPVTYNQIPVITTELLARLYGTEAIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELKNLRLVLNESQNEVKISPKTRTLILWTERGAARHAKMLET
                     DQAWEVFEKLEDSYFNQYEKERAMGKMNIDFHLAIRDDKTAFIRSYAPGQLVTVEEAL
                     ALLKSRGWLVMPRDELAGKLMEL"
     gene            3187..3342
                     /gene="hokD"
                     /locus_tag="ACHY5W_004084"
     CDS             3187..3342
                     /gene="hokD"
                     /locus_tag="ACHY5W_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4448859.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(3445..3762)
                     /locus_tag="ACHY5W_004085"
     CDS             complement(3445..3762)
                     /locus_tag="ACHY5W_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448860.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAITKQTMREFDETCLQPAPV
                     MSPERIRALREREHLSQPVFARYLNVSKNLISDWERGVKRPGGAALRLLSVVEKNGIQ
                     VIS"
     gene            complement(3755..4126)
                     /locus_tag="ACHY5W_004086"
     CDS             complement(3755..4126)
                     /locus_tag="ACHY5W_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4448861.1"
                     /translation="MRIFKNAWFERFARKHRISDKSLRKIVEQADKGIISANLGSGVI
                     KQRLARSGGGKSGGYRTIIFYRVAEKAFFIYAYAKNERENITAIEENAFRKAAPHVLN
                     LTDEQLAQLIQQGQFTEVPNE"
     gene            4383..4577
                     /locus_tag="ACHY5W_004087"
     CDS             4383..4577
                     /locus_tag="ACHY5W_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448862.1"
                     /translation="MKIPASLIPGFYESTRPVVVYRNSDGSFQCGFVMRSNEVVVSLS
                     LLSEVRELAGLPVDDIQKQL"
     gene            4631..4900
                     /locus_tag="ACHY5W_004088"
     CDS             4631..4900
                     /locus_tag="ACHY5W_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448863.1"
                     /translation="MAHDSRLHNSAALASRQGRCSHVFKTDWFQHPLCTEEQAEWLIQ
                     CYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            4902..5951
                     /locus_tag="ACHY5W_004089"
     CDS             4902..5951
                     /locus_tag="ACHY5W_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4448864.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            5965..6717
                     /gene="ydfT"
                     /locus_tag="ACHY5W_004090"
     CDS             5965..6717
                     /gene="ydfT"
                     /locus_tag="ACHY5W_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS4448865.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSSLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
BASE COUNT         1799 a         1575 c         1903 g         1639 t
ORIGIN      
        1 cagtgagcag gaagttttgt cgttacaggc agccccccat gtattcctgg atggcctgag
       61 cgaacccatc ggaaaatttc cgatgaccga tagctggtat ccgtcacggg attttcgacg
      121 acgggctgcg ttgtggggga tggctttgcc ggagacagaa tttacacctg ctgaacttac
      181 cgcattccgg gactactggg cagcggaggg gaaagtgttt acgcagattc agtgggagca
      241 gaaattcgcc cgtcacgtaa atcacgtcag ggcgcaggtt aaaccagtca gcaagggggt
      301 aaaccatgca gcagcaccag gtggcaccgc atcacgggca gttcaggaaa ttcgggcagc
      361 acgtgagcag tgggaacgtg aaaacggatt tatcagcgac ggaaacggcc tggaagctgt
      421 gggaactcat gggggaggtt tattcgaacc gctggaccca gaagaacggg gccgcacctt
      481 cgaagctctg gattgcacag attggcgcga tgactgagca gcaaatccgg caggtctgcc
      541 gccagtgcat ggaccgctgc cgggcgggtg aaacatggcc tccggacctg gctgagtttg
      601 tggcgctgat ttcggaaagc ggggccaatc catttggcct gacggtggat gctgtgatgg
      661 aggagtatcg ccgctggcgt aatgagtcct ggcgatataa cggaagcgac aaatatccgt
      721 ggcctcagcc tgtgctgtat cacatttgcc tcgagatgcg ttcaaagggg attgaacgcc
      781 agatgaccga aggggaatta aaacggcttg cagaacggca gctgacgaaa tgggcaaagc
      841 atgttagtaa cggcctgagc gttccgccag tacggcgaca actggcggca ccaaaacgcc
      901 cgttgggacc aacgccaatt gagttgctga aacaggagta tgaacgccgg aaagcggctg
      961 gttttgtctg atttgagaag taatttttat ccggaggaag ttttaatgga aaccgtattg
     1021 catgcactga aagcgatggg taaagccaat tctgttgaac tggcggcacg aattgatatc
     1081 agccgtgaag aagttctcaa cgaactgtgg gagctcaaaa aaaatggcgt tgttgataaa
     1141 acgggtcaca cctggtttct ggctgtcgaa ggtgaatccc gggtaaccga agagcggcca
     1201 gtaaaatctg aaacacagga tatgctgacc gaagaggtcg ctccaaaagt tagcgctaac
     1261 atgatgattg agtttatcgc tcaggagggg gctaaaacct gtgaagaaat agcgggtaag
     1321 ttcagtgtca gtactcgcaa ggttgcttcc acgctggcgg tggtaaccgc aacggggcgg
     1381 ctggcacgcg ttaatcagaa cggtagattt cgttactgca tgccgggcga taatttacca
     1441 gcagagccga aagccgcgct ggtaacggaa aatgatggta aggcctttcc tcagccagca
     1501 tgtgttgcgt taccagtccg ggaagcggaa acacaggaag aaataaaaac ggaaagtgtg
     1561 gcggtcacag tgcagtcaca gccgtcgttc accagaaagc atccggatgg gctgatttta
     1621 ccatcgctgc atgtggctaa ccgcgagctg cgccgggcaa aaggtcaggt tcagaagtgg
     1681 gagcgagtct gcgccgcgct gcgggagctg aacaagtgcc gggatattct ccgggatatt
     1741 accgccacca gagaacagca gcggtgagtg ggtggaagac gtggtgccgg gctgaaattc
     1801 tgatactccg gcagtgcgcg ggaacgatga ctgtcgaaag tatcggcagt ctgattggtc
     1861 gtagcgaggc agcggtcaga gcgaaagccc gggataaggg aataagcctg atgctgcagg
     1921 gtgattttcg cctgtcagcc aaattaccgt ataaagcgat ggactgcctg aagcgaaacg
     1981 gactgattgg cgctgaggat agtttacgat gacctggcct gaggcattcg taacggcagg
     2041 agttgcaatg gcggtagcgc tggtggttta ttcgatttgc cgctggggtt gacgtaatta
     2101 ctaatccggg gctatattca ccgcacggtc gagttgaccg tcgggattga gaccccggat
     2161 attcaaccgc gacagacaca cgccgcgagc gtgtttttta ttgtcgtatg cgcacgcaca
     2221 tctgaattat ggtgaggcgt atggggcagc cgaaaggctg gccggttggt tgatccggta
     2281 gtctcaaccc tgtacgtctc gccacctgat gattgagacc tgaaggtggt gatagttaaa
     2341 ttcatcaacc agagggcgtt atcatgacga ctcaagttac tgtcaaaaac ctttcccctg
     2401 tcacttataa ccagattcct gtaatcacaa ctgagttact cgcccgcctt tacggaacag
     2461 aagcaatccg tatacagcag aatcaccatg aaaataaatc tcgatttatt gagggaaaac
     2521 acttctttaa agctgttggt gatgagctca aaaatttgcg actagtttta aacgagtcgc
     2581 aaaatgaggt gaaaatttct cccaaaaccc gcactctcat cctctggaca gaacgcggag
     2641 cagcccgtca tgcaaaaatg ctcgaaaccg atcaggcgtg ggaagtattc gaaaaactcg
     2701 aagacagtta cttcaaccag tacgagaaag agcgggctat gggaaaaatg aatatcgatt
     2761 ttcacctggc gatccgtgac gataaaaccg ccttcatccg aagttatgcg cctggccagc
     2821 tggtgacggt tgaagaagcc ctggctttgc ttaaatcgcg cggatggctg gtaatgccac
     2881 gagatgaact ggccggaaaa ttaatggagc tttaaccgat tgagttaacc ttgatatggg
     2941 aattcccata tcgggagaac attaccatcg gggataaaat gatttgcggg gaaagaagag
     3001 ttaagtagaa ttgctgcggg tgcttgaggc tgtctgcctc gggcatgccg ccgtaaggca
     3061 gacagagaaa agccccagtt aacattacgc gtcttgcagg acgcttaaca ttaatctgag
     3121 gccaatttca tgctttgcac atgtaggtta gcctcttacg tgccgaaagg caaggagaag
     3181 caggctatga agcagcaaaa ggcgatgtta atcgccctga ttgtcatctg tttaaccgtc
     3241 atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag
     3301 acggaggtcg ctgtcttcac agcttacgaa cctgaggagt aagagacctg gcgagggaga
     3361 aatccctcgc cacctctgat gtgtcaggca tcctcaacgc acccgcactt aacctgcttc
     3421 ggcgggtttt gttgtattag aatatcagga tattacctgg atcccgtttt tctcgacaac
     3481 tgaaagaagc cgaagagctg cacctcccgg gcgtttcact cctcgttccc agtctgatat
     3541 caggttttta ctgacgttga ggtatctggc aaaaacaggt tgagacagat gctctcgttc
     3601 tcgcagtgca cggatccttt ccggagacat taccggcgca ggctgaagac aagtttcatc
     3661 gaattcgcgc atagtttgtt ttgtgattgc accgatatca tggagtgact ccatcatttc
     3721 atgtacagat gcaagtgcat cactgcggta atttttactc attgggtacc tccgtgaact
     3781 ggccttgttg aatcaattgt gccagctgtt catcagtaag attgaggaca tggggagcgg
     3841 cttttcgaaa agcattttcc tctatagcag tgatattctc tcgttcattc tttgcgtatg
     3901 cgtagatgaa aaaggctttt tctgcaacgc ggtaaaaaat tattgtccgg taaccgcctg
     3961 attttccgcc accacttcgg gctaatcttt gtttaatgac accactaccc aaatttgcgg
     4021 atatgatccc cttatcggcc tgctccacga ttttgcgcag cgatttatcg gaaatccgat
     4081 gttttcgggc gaaacgttca aaccaggcgt ttttgaaaat tcgcatacct gcctctgcat
     4141 tttatataac acatagtgtt acaccatgtg gactatggag gcaatcttta tttgagaatg
     4201 cttgctgatt tatcggtgtt caaaaaacag acgtaattca gattgtataa acggcagaca
     4261 gaaaaagtca ttgttcttgc gcataaaatc ctcagattta ctgagtgaaa tgcgtctctt
     4321 ttcatggtta tagtccgaga gaagcgataa tgcatttcat ttcctttacg tcaggaggtg
     4381 ttgtgaaaat cccagccagt ttgatcccgg ggttttatga gagcacgcgc ccggttgttg
     4441 tttacagaaa cagtgatggt tcttttcaat gtggttttgt tatgcgaagt aatgaggttg
     4501 ttgtaagcct ttctcttctt tctgaggtaa gagagttggc aggattacct gttgatgata
     4561 ttcagaagca gctataaaat caggctgact tgaaccgtcg ctgagaaaca ccgtgccacc
     4621 ggagatcccg atggcgcacg atagcagatt gcacaattct gccgcccttg ccagtaggca
     4681 agggcggtgt tcgcacgtat ttaaaaccga ctggttccag catcccctat gcactgaaga
     4741 acaggccgaa tggctgatcc agtgttaccg caggcgtggt tacgagtttc agaaagatct
     4801 cagcttcgat cgtcgtcact ggataatctc cgccaggctc ccttattccg aacgcccacc
     4861 gcgtccgtcc cgcacattcc agcagcgcat ctggaggtaa cgtgcgggta ttacttcgac
     4921 ctgttctggt accggaactc ggtctggtta tcgttaagcc aggccgtgaa tcaatgtcag
     4981 tattccataa cggcagaata ttggtggagc cggaaccgaa aaacatgcgc ggtctgccgt
     5041 ccggagtcgt tcctgccgtt cgccagccgc tggcagagga taaaacatta ctgccatttt
     5101 tcagcgatga gcgggttatt cgtgcagcag gtggtgcagg tgcactgtct gactggttat
     5161 tacgtcacgt gaaatcctgc cagtggccac acggcgatta tcatcacagc gaaacagtca
     5221 ttcaccgtta tggtaccggc gcgatggtgt tgtgctggca ctgtgacaac cagctgcgcg
     5281 accagacatc agaatcactc gagcaacttg ctcaacaaaa cctgtcagca tggatgattg
     5341 acgtcatccg ccacgcaatg aatggcatac aggaacggga attatcgctg gctgaattat
     5401 cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta ttacgtcgtt
     5461 cgctggggtt gcgtgcggaa aaaatccgct ccgtataccg tgaaagcgac atcataccgg
     5521 gagaacagac cgccaccagc atactgaagc agcgcacaaa aaatattgcg ctaccgtctc
     5581 acacccacca gcaacagaac ccaccacagg aaaagacggt ggtcagcatt gccgttgatc
     5641 cggagtctcc ggaatccttc atgaaacgac ctaaacgtcg ccgctgggta aatgagaaat
     5701 acacacgctg ggtaaagaca cagccgtgtg cgtgttgtgg taagccagcc gacgatcccc
     5761 atcacctgat tggtcatggt cagggcggaa tggggacaaa atctcacgat attttcacgc
     5821 taccgctgtg tcgggagcat cacaacgagc ttcatgcgga taccgtggca tttgaagaga
     5881 agtatggctc tcagctggag ctgatatttc gttttatcga tcgtgcgctg gcaattggcg
     5941 tactggcgta agtggagaac gagcatgaac cttgaagcct taccgaaata ttactccccg
     6001 aaatctccaa aactgagcga tgacgcaccg gcgacaggct ctggtggttt aacaattacg
     6061 gatgtgatgg ctgcgcaggg gatggtgcag tcaaaagcac cgcttgggtt tgctttattc
     6121 ctggcaaaag ttggtgttca ggatcctcag tttgcgattg aaggtctgct caattacgcg
     6181 atggcactgg ataacccgac attgaataaa ttgagtgaag aaacccggct acagatcatt
     6241 ccttaccttg tgaattttgc ctttgctgat tattccaggt ctgcggcaag taaggctcgc
     6301 tgtgagcatt gtgctggtac tggatttcat aatgtattgc gcgaggtggt gaaacactcc
     6361 agaagcgggg aatctgttat taaggaagag tgggtgaagg aactatgtca gcattgtcat
     6421 ggtaagggag aagtcagcac agcatgcaga gggtgcaagg gtaaaggtat tgtcctggat
     6481 gaaaaaagaa cccggcttca tggcacgcct gtttataagg tttgtgggcg ttgcaatgga
     6541 aaccggttta gcagtttacc aaccacactg gcgcggcttc atgtccagaa gctggtacca
     6601 gacctgacgg attatcagtg gtacaaagga tatgcagatg tcattgataa actggttaca
     6661 aagtgctggc aggaagaagc atatgcagag atacaattga gaaaggtgac aagataaatg
     6721 gttttcgccg aagatgacga catgatgctt gcatttttca aaaaatatgg ataagatttt
     6781 cccaacgatg ggctttgtat gtctaccgtt gataagattt aagaacccgc cgatgcgcgg
     6841 gtttttttgt acccagaatc ctgtgagcta tacggaaagt acacagaaag gaaggtgcga
     6901 ccacaattaa taacaa
//
LOCUS       DAZUGK010000222         6906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZUGK010000222 DAZUGK010000000
VERSION     DAZUGK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.223"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2745)
                     /gene="pqqL"
                     /locus_tag="ACHY5W_004091"
     CDS             complement(<1..2745)
                     /gene="pqqL"
                     /locus_tag="ACHY5W_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4448866.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKAWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLTEKWQQLRKNQ
                     DAFWEQMVNNEVAANKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGELSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     H"
     gene            complement(2802..5174)
                     /gene="yddB"
                     /locus_tag="ACHY5W_004092"
     CDS             complement(2802..5174)
                     /gene="yddB"
                     /locus_tag="ACHY5W_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS4448867.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSFLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5212..6897)
                     /gene="yddA"
                     /locus_tag="ACHY5W_004093"
     CDS             complement(5212..6897)
                     /gene="yddA"
                     /locus_tag="ACHY5W_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4448868.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKKNWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKFAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1778 a         1586 c         1564 g         1978 t
ORIGIN      
        1 atgagaaaga tattgtttaa cggcagtgtt aacattctct accgtcattt gtttcaacaa
       61 ttgctcctgc tcagtccatg ctgcaggatc gtcatattga ataagactat ttacaatagt
      121 gttcgctaat tgttgaacgc tacgctgttg gatatcgagg ctgcgctgaa cgttttgctg
      181 gtattcgttc agttcttgct cactgatccc tttagccaga cgcttaacca tcacttcatt
      241 cgctaacgtt aacagttcat catgtcgttc tggttgacaa gtaaaagcca gcaaatgact
      301 gatatcttta gcctgaggat caaccgagag gcgagaagaa acgctgtatg ccccagatgc
      361 ctgttcacga atattaacgc gtagatcttt tgctagtgcg acgttaaaag catcgagcgc
      421 catacgcgtc gccagattaa caggtgtccg ggaatcataa cgcttccact gtgaaacctg
      481 tgccacaggt tcattttgtt cttttacagt aaccgatgcg ttgtccgtcg cgcgagttaa
      541 tggtttacct gcggctaatg gcgaatcaga gtgtttgatt gagcctaagt aacgcgtaat
      601 taacgccacg agtttgtctt ctgcgacatt accgacaatg acaaacgtga tatccgctgg
      661 agatgaaaac aactggcgat cggcagccag cgcatctgcg gcagtaaact gtgcaatctg
      721 attttcttgc agtaatttcg tgcggtcatc agcatagcgc gtctcataca tctgctgggc
      781 gaatttctcc gctggacgct ggtcgagcgt tttcaacgcc tgaatttgag cattttgtag
      841 cgatgcccaa atattgtcgt taatcgtgct gtgggtgatt cgctggttaa tcaactggaa
      901 accaggttca gggttattag tccgcgcgct aacggagagc aatgtattca tgccactgac
      961 tttactgctc atggttaccg agttttccgc actccagcgt ttcaggctgg aagaggagag
     1021 ttcaccaacg ccgcttccgc ttactgcttt atttgccagc gcgataagtg atttttgctg
     1081 cgcagggaaa cttaactcgc ctttattcga tacggcagta atttgtagct tttgctcttc
     1141 acccgcggat tttgccagaa taaccctggc accattggaa agtgttaatg atgtcagatt
     1201 ctcagccagc gtttctttgc tgctaatttc cgcctgtggg tcagcgtcta ctgttaacga
     1261 taaatttctg cctgggaaga tgtaagccgc cagctttttg ttggcgtact ctttttccag
     1321 cgccaggata gccgcaggag acaatgcttt attggcggca acctcattgt ttaccatttg
     1381 ctcccaaaat gcgtcctggt tctttcttaa ctgctgccat ttttccgtca gactttgcac
     1441 ggtaatttgc tgccacagac gtttcgaaag ttgatatgtc tcttccggcg acaagaacgg
     1501 cgtattattt aatgagctgg atgccaggcg actggtcagc atacgtaaat cacgttcagc
     1561 ttgctgatca accgcatttt tcagccaggt gaggcgggta gatttgacat catcgagttc
     1621 ttcagcagaa aaaccatgct gatcaatggt tgccaactct gccattaatg cattcgcagc
     1681 atcctgcata ttatcgtctc gtgcatttac acggaaaaac agcgactgat aatcgggtgc
     1741 aattttaacg ctgcgcgcag tgccgccaga aatagtcttc aactcgcccg actgtatgcg
     1801 ttcctgcaga cgttgattga acagctgaac taacatgctc cattcagctt gttcgataaa
     1861 gctttgctca tcgttcactt gtaccattgg caggcgataa tagagtgcga tgccgttcac
     1921 ccggttttct ttatcattaa tgatattaaa gcgcaggtga ttttcggctt ttgtcggcca
     1981 gacgcgattt tcagctgctt tgttagccgg aagcttactt aaattatcct ttatcagcgc
     2041 cagcgcttct ttactgtcga tatcgccaac cacgataaag gtcatattat ttggttgata
     2101 ccagcgttga taaaattggc gcaattgtgc cggtgtgacc gtggcgacag tatccatcag
     2161 gccgataggt tcacggtcta aattacgggt attagccagc aggaaagggc ggcgcgcctg
     2221 agaggtgcgc catttcgcat cctgatgggc acgccattcc tcagtaatta cgccacgttc
     2281 agcgtctact tcgagttttt caaaggttgc ggcattactc cattcactga agattgccat
     2341 cacttgttgc agattttgtt tctgcgtagt cggcaaactc acctgataca ccgtttcgtc
     2401 atagctggta taggcattaa catcgcgacc aaaacgcagg cccattgact caaatgtttc
     2461 gatgacttta ttacccggcc atgcttttgt gccgttaaac atcatatgtt ctacaaaatg
     2521 agccacgccg cgctcattgt cttcttcctg caatgaaccg gtatgaattt gcagccataa
     2581 atttacctga tcctttggat gagcatgcgg ataaatcata tatcgcaagc cattgtccag
     2641 ttgtccggta attaactttt catcctgcgg taaggcggcg gcaatcagcc gcccggggag
     2701 caacagagtt gccactaacg tcagtaagaa acagaggttt ctcataatta tctccatgcg
     2761 aaaaccgggc gaatttaccc ggttaagtaa aatccgaact attaaaattt catgctgaca
     2821 tcaagccaga aagtacggcc actggcgtag gtcgccacac cggtattcgt tgtatcaaca
     2881 gcggttttgc tatccagtac attgagaata tcggcactga tcgtcaggtt ttgttgtttc
     2941 agaaattgtg gcgtccagga caagcgggtg tcccaggtca ggctgctatc cagtttttcg
     3001 tcaacatact gcttgtaatc tgaatattcg ctgatgtatt gcgcattcgt cttacccagg
     3061 ataatgcgag ctttacgcgc ttcttgccag gccagcgtat tcgcccacac caacccgctc
     3121 ggttgatgcg tgaaatccat gtttaaggag atctttaatg ggttattaaa atccgccact
     3181 ggaacgctat cgtaagagac aagattaccg ttataaacca cctggttatc accggtattg
     3241 ctctcctcat aaccattgtt taacgacaag ttacccttgc tcttgatata gctaaagaca
     3301 atttgcgggt taatatctac ctggctgata tgcaggggtt cggccagttc aaaactgagg
     3361 ttgaacgaat gcgttttggt tttgccgtcg ttgttatatt cagtaatggt ggttttagtc
     3421 gcgctgtcgg tacgactgct tttgctgatt tgatcatgcg cttcacggta aacatagttt
     3481 gcgcgcgcaa taacgttctt accgattttt tgctgcaatc ccattgccag ttcatcgtta
     3541 taaggtgttt tcaaatcctg ataacgcgtc agggttttat tacctgatac cgattccgtc
     3601 cagctattgc ggatatcacg taatcccata tcaagaatat tcccgccata gtaacggtta
     3661 taacctgctg taatcattga ggtttgatca gcaaaaatat cccattccgt cataaagcgc
     3721 ggggagatat tgtggtttga cagatagttg tcatagtcat accgcacgcc gggcattaat
     3781 gacacattac gccagctaat gcgatccgcc atatacagtg tgtagttgtc aattcccagg
     3841 ctgcctttac ctttatggta aatggtatgg ttagttttct ttccggcagc attaatcaca
     3901 taggattcag actggttatg gcgttcagtc catgcatcgg aatagatgta ttccgcgccg
     3961 aagtagggtt gatgcgaaac attacccacg gcgaattttt gccagtccag gcgtgttttg
     4021 aaggtgtaat tatctacagc ctgggaaatg tgtcctaatc cgccacgggt gcaacgccct
     4081 gtaatatcac catatgtaca tgaaagttcg gtgtaccaga tgtcatgatc gtgacgggta
     4141 taatcactaa tatgatccca accaacggtg gtacgtagtt tggcccatgc gagctgcgta
     4201 tccatatccc atgccagacc ataggattta ttacccattt cgcgatcaga ctgcgggaag
     4261 gtgctggtat tataatcacg gctggagccg gtatatttta aggttaaatc gtgggtaaag
     4321 cggtcgctgg caaaccaggt aaatttgctc aatgcagtat cgataacgtt tttatactgt
     4381 gcccgaccgg cgacaatgcc gtcattcgaa acataatccg cgcgggtgat atcagactgg
     4441 cggcgcgata aaccggcggt aacgccaaag ttatcagcga gttcctgatt aaacgacaag
     4501 gtataaaagt tctttttaaa atctggggag taataagtac ttcctgaaga gccttgatta
     4561 aatgcgctct tgttattctc atcgatatgc gatgttaacc agtccgaacg cgtagtgcga
     4621 taacccagtt tcacgctgct atcatcagcg ttgaagcgtt tgatctttgc atcaattacc
     4681 ccgccattga agcgaccgaa ttcaaccggc acaaaactgt cataaagcgt cacattgtcc
     4741 aggaagctga catcaagata atacccctgt gacattccgc taatattggt tgcactactg
     4801 gcatcggact cattcgctgg gttcaggtta ttagtggcac taataccatc aatcaaatag
     4861 gcattctggt agggcgacgc accgtgaata gagattttct ccgggcgaat atctccctgg
     4921 ttcaacgagg tactttgcgt tgaatccatg cgtaccgcag ggttggttct cagcagatcg
     4981 ctgatattgc cattcccggt cggtaaacgc tcgatgcttt cactggtgta atgcgtgttg
     5041 ccgtttacgg gtacagggac aggagcatag accgtcatct cttcaaaaac atgcatgtac
     5101 atgtttttgt catcgacggc ctgcgccaca tcagcgccac ataaaatgac gccaggaata
     5161 agaactcgct tcatttattt atatctccgt acagcagggc ttttattgtt tttataaaac
     5221 cgcgctaatg tcacaaatat catcggccag gttccagaca ccgggttgat gtgtaaccat
     5281 aatgacgccg cttgtgggta gtttttcacg cactaaacgc agtaagcgga tagcctcttg
     5341 ttcctcaaga tgagaggtag tttcgtcaag aaatatccat ttcggacgtc gtaaaattaa
     5401 tcgtgccagg gcgatacgtt gtttttcgcc gctggaaaga atatctcccc agcgatcgtg
     5461 gtcatgaata cgcgcagcaa atttccccag accaacctga tgcagtactt cgctcaacga
     5521 tttatcgtct acgggcaggg gaagtgcttt acaaataatc tctttcagta agccggtttt
     5581 gattaacggt gtttgtgaca cataccaact gtcagcagga gaagaaatat cacctttaaa
     5641 ccacggccag cagtgagata atgttttaag cagtgtggtt tttcccgcac cagagtaacc
     5701 tttcagtaat agccatttgc ctggcggaac atgaaagttc aggttctcta atatgatctt
     5761 attatcaggg gtacgaatac tcgcgtcagc cacttgtacc gcatgttggc aatttttagg
     5821 cttattcgta gggcgctgtt cggtgagttg atggaactca tacaagcgat cgataaccgc
     5881 agccagttca gcaagttcat catatttata aataaaccag cttaaattgt tcgataccag
     5941 cataaatgcc tggcgcgatt tcatcagtcc gcccagattg atctgcccgc taataaactg
     6001 cggcaataac agaaagtagg gaagaacgct aagcgagcgc gaataaatat tctgccagta
     6061 atcaagccac cgctgacgat tcattaaacg atgccagttc tttttaatgg tatgaaaatt
     6121 gtcgcttaac tcctggcgtt gtagagactc agcattgctt agcgcaatca gttctgcctg
     6181 tttattatgc tgcacaagat tagttcgaaa tgtcgcttcg ctacgttgtt tttccacatt
     6241 aagcggacga atacgtttac ccaccttatg ggtaaataaa gttccaccaa tcacaatgag
     6301 cacgacggta tagaccatat acccctggat attccattct gttccaccaa cagtgaatga
     6361 gagcgtaccc gcgctttgcc aaagaataac ggtaaaggtg atcagcatgc taagtgactg
     6421 gatgaagcca aatgaaaggc tgagcgtttt gctgatcagt aagagaatgt cttcagcgat
     6481 acgttggtca gggttatctg tatttttatg ctcaccgtag atctgcgtga agtagtagtt
     6541 tttatctgca aaccagcgat taaggtagta atctgttaac cattcgcgcc agcgaatagt
     6601 cagtagttta attaaccatg ttttgtttac ggatattaat acaaagatcc ccagcagggc
     6661 agggaaccaa aggacaagct gccaaagctt atcggtttct ttctggctta gcgcattgaa
     6721 aaaatcatta ttccagtcgt ttaaccagac ctgaatttta accacgccaa ggatcatggc
     6781 gaggataata atgattaaca acactgaagt tttattattc ttacgcagcc aaaagggctt
     6841 cagcagacag agatactttg ctatcaacat acaaaacgta atgggaatgg ttatcattag
     6901 cgaaaa
//
LOCUS       DAZUGK010000223         6888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZUGK010000223 DAZUGK010000000
VERSION     DAZUGK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.224"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(190..675)
                     /gene="sixA"
                     /locus_tag="ACHY5W_004094"
     CDS             complement(190..675)
                     /gene="sixA"
                     /locus_tag="ACHY5W_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFS4448869.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(878..3022)
                     /gene="fadJ"
                     /locus_tag="ACHY5W_004095"
     CDS             complement(878..3022)
                     /gene="fadJ"
                     /locus_tag="ACHY5W_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFS4448870.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQKLMAEIHALPV
                     PVIAAIHGACLGGGLELVLACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLLEMSERGESFWKTSATDPH"
     gene            complement(3022..4332)
                     /gene="fadI"
                     /locus_tag="ACHY5W_004096"
     CDS             complement(3022..4332)
                     /gene="fadI"
                     /locus_tag="ACHY5W_004096"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFS4448871.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPDAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4514..4798)
                     /locus_tag="ACHY5W_004097"
     CDS             complement(4514..4798)
                     /locus_tag="ACHY5W_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFS4448872.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5170..6510
                     /gene="fadL"
                     /locus_tag="ACHY5W_004098"
     CDS             5170..6510
                     /gene="fadL"
                     /locus_tag="ACHY5W_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFS4448873.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
BASE COUNT         1764 a         1815 c         1600 g         1709 t
ORIGIN      
        1 tatgatttgt aagaatatat tacaacctag tctcatttca tcagaaatta atagggaagc
       61 agaggaaaga gtgcgatgaa atcatcatcg cagcatacca actccgtgaa gggtttttag
      121 tgaatttata ctattgcaaa aagacaaatg cccgatgtac agcttacatc gggcattttt
      181 tcaaacaaat cagatagctt tcgccatctt cagattacac gggctcatct gccagttgaa
      241 tgtgccgttt ccgctttcat caagcgttac gctcgcaatg gccgaggtgg taaacatagg
      301 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgggagat
      361 aaccaacacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
      421 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
      481 atcccctact tcctccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
      541 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
      601 agtggtaaga ggacgaaccg aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat
      661 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
      721 gaaaccagag aaagaaaacg gtgtccggca ttgtgcctta tccattcacc gaatgaaacg
      781 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaataat
      841 ccgccattca gcgcggattc atatagcttt gaccctttta atgagggtca gttgcacttg
      901 ttttccaaaa actttccccg cgctcgctca tctctaataa acgctcgcaa ggggtaaaac
      961 gagaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
     1021 aatcgatata gcgaaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
     1081 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
     1141 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcgc ccctgccctt
     1201 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
     1261 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
     1321 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
     1381 taattttagt cccggtgtcg attcctacct catccaaaag ttggattgga cctaccggaa
     1441 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
     1501 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
     1561 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
     1621 gcgctgatgt acccgcatga ggaataatct ccaccagcgg cattttttcc accggactga
     1681 agaaatgcaa gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
     1741 gtaaagatga cgtattcgaa gcaaagatgg tatgagcggc gcaattttgc tcaacttccg
     1801 ccaccatctg ttgtttcaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
     1861 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcac
     1921 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
     1981 catgatttat gccctgcggg ttgatatctt taattctgac cggaatcccc gctttacaag
     2041 cagtgacata agcaataccg ccgcccatca agccaccacc taaaatcccc acgctgttta
     2101 atggcgcagg cggcgcatca ctgccgggat ctttcttcac ttccgtactg gcaaaaaaga
     2161 tactacgcaa agcttgcgat tgtggcgtca tcgccagttc gccaaacgcc cgtgcttcag
     2221 cgtcatagcc actgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
     2281 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
     2341 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
     2401 atgggcgatc cttctttgct agctcaacag cggcttccag cagaatggag tgcggaacaa
     2461 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagga
     2521 tcatctctaa tgctgtgctg acgcctatca gacgcggtaa acgctgggtg ccacctgaac
     2581 cgggtaacaa tccaagttgt acttcaggca aaccgagcac cgttttagga tcgtcagtac
     2641 aaacgcgacc gtggcacgcg agcaccaact ccagcccgcc gcccaggcaa gcgccatgaa
     2701 tagccgcgat aaccggaacg ggcaaagcat gaatctctgc cattaacttt tgcccctgcc
     2761 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc gatcatgttg atgtctgcgc
     2821 cagcaatgaa gttgtccggt ttagcggaga caaacaccac gcctcgcaac tctttgtttt
     2881 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ctccgccttc agggtattca
     2941 ttttctcacc cggtacgtcg atggtgataa cggcaatgtt gtccagacga acattaaggg
     3001 taaacgctga tgtcatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
     3061 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
     3121 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgc ccgtaagcaa tcgaaccgcc
     3181 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
     3241 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaagc
     3301 ttcgtgcata tcgatcaatg tcagatcgct catcgtcaaa ccggcacgtt ccagcgccag
     3361 cggtgttgac caggccggac cgagcaacat gtcctgccag acatcgatag cggtaaatgc
     3421 gtagctgcgc agatatccca gcggtaccag ccctaattct ttcgcccggg attcagtcat
     3481 caggatcacc gccgctgcgc catcggtcag cggcgtactg ttcgccgccg ttaccgttcc
     3541 gtgtttacga tcaaacgccg gacgcagctt tgcgtaatca gcaagcgagg aattaccgcg
     3601 aatattgttg tcttccgcga gcagttgttt ataaggaggg ataaaggcag tcatcacctc
     3661 ttctttgagt tttccctctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
     3721 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgctctg cggtgtcgcc
     3781 catccgcaag ccggtagaat attctgctac cgcaggtggt acgggcatta agtcgcgcaa
     3841 acgcaggcga gagaagagtt tcagtcgctg gctcatagtt cgagctttgt tgacatcaac
     3901 cagcacgcgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
     3961 accggcaatc cccgctcgaa tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
     4021 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
     4081 ggtaccgaga acaatttcac gcgcaatgtt gggggcatca ggcatttgta cgacctgacc
     4141 aaagaccagt tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
     4201 caccatcttc cccaaatcaa cagcaggaat gccatgaaaa gccgtcgcct ggcgggcaaa
     4261 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagcggtaa
     4321 aacctgaccc ataacactcc cctgtaaaaa aaataaataa agtggtctga cctgatcata
     4381 gtcttaacca tttttttaca tttagccaag cggagaaaag ggaaagtggg agctatgaca
     4441 cagagagaaa agaagaagag aaaagaaaac gcccctgcca gcaattgaca ggggcgtacg
     4501 tacccgagag gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa
     4561 aagtgaaatc gatatccagg cgcacaccat cagactcttc agtgaaagtc ggggagattt
     4621 tgcacggttc ggattccacg ctacgggctt tctcagtcag cgccgccagc gtttgttctg
     4681 cttctgcgcg gtttgcaaac acgcggctgt aagacgcggt gcagtcggag ttgtccataa
     4741 tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg
     4801 ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt
     4861 ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac
     4921 ttaaaaatga tctaaaacaa aattcacccg aatccatgag tgcgccacct ccaaattttg
     4981 ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tagaaacata cttgcaacat
     5041 tccagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc
     5101 ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag
     5161 gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca
     5221 cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc
     5281 ctgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgtcagccgt
     5341 aaccccgcat tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt
     5401 gatccggatg tagatatttc tgggcgttcc gatttaacgg gccagcgtgc tgatgcagat
     5461 aatattgcac caactgcgtg ggtgcctaac ctacatttcg tagcacctat taacgatcag
     5521 tttggttggg gggcttctat tacctcgaac tatggtctgg ccacggaatt taatgataac
     5581 tacgctgctg gaatctatgg cggtaaaacc gatctgcaga cgatcaacct taaccttagc
     5641 ggagcctacc gccttaacaa tagctggagt tttggtcttg gttttgatgc tgtatacgct
     5701 aaggcaaaaa ttgagcgtac tgccggttca ctcggtccat tgctttccca acaactgata
     5761 gcgcaaggcg tgccacaaag ttggacaaac ggcatcaata caccggattc gcaaattgct
     5821 catctcaagg gtgatgaatg gggattcggc tggaacgccg gtattcttta tgaactggat
     5881 aaagataacc gttggggcct aacctatcgt tcagaagtga aaattgactt tgaaggagat
     5941 tacaaaagct ccatcaaccc aaatctgaac aacattttgg gtagcatggg tttaccatat
     6001 ggcacaatgg gtgcaaccca aaatgggtcc ttaacgctga atttacctga gatgtgggaa
     6061 atttctggct ataaccgtgt cgcaccacaa tgggctatcc actatagtct ggcgtatacc
     6121 agttggagtc aattccagga actgaaagcg aaaggagact ctggtcaaac gctgttctat
     6181 aaagacgaag gtttccggga tgcttaccgt atcgcgttag gtacaaccta ctaccacgat
     6241 aaaaactgga ccttccgtac gggtattgca ttcgatgaca gcccggttcc ggcagataaa
     6301 cgttctatct ctatcccaga tcaggatcgt ctgtggttaa gtgcaggtgc gacttatgca
     6361 tttaatgaag atgcatccat tgacgttggt gcatcttata tgcacggtca gaatgtgaag
     6421 tttactgaag gtgaagggcc agcagcatat acctttaaat ctgaaggtaa agcctggctg
     6481 ttcggtacta actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc
     6541 tatgcaggtg actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc
     6601 tgagttttgc aaacagcctg atggctgctt gccctttcaa atcagtaagt ggtatccaca
     6661 gacaaacctg aagggaaacg catattttca ggcgttctgc tcgtcctcct caaagagttt
     6721 acttttctgc atttccagga tactcaacct ccctggctat tgtgcgctca ttcactcaaa
     6781 ttaaagatag gttctaaata aatgagcgtt ttttgatagt ctatttcatt aggtaatata
     6841 tatttgtaac aaatcaatca acatggaata aaatcatgct accatcta
//
LOCUS       DAZUGK010000224         6873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZUGK010000224 DAZUGK010000000
VERSION     DAZUGK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.225"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..1219
                     /locus_tag="ACHY5W_004099"
     CDS             92..1219
                     /locus_tag="ACHY5W_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFS4448874.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILLEMLPPSTRRLYW
                     RVEDDLALVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKLRMKPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1225..2016
                     /locus_tag="ACHY5W_004100"
     CDS             1225..2016
                     /locus_tag="ACHY5W_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4448875.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYGVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPTWFSPLRARWHYYSMAQKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFSELLAGSELDAALRAEIDELLERKQRAGEAEYGP
                     RRSLLHAFIHAELARGEIPPVLPDSREGDVKKLDSLLYQTVMRRA"
     gene            complement(2049..2977)
                     /locus_tag="ACHY5W_004101"
                     /pseudo
     CDS             complement(2049..2977)
                     /locus_tag="ACHY5W_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3122..4471)
                     /gene="lysC"
                     /locus_tag="ACHY5W_004102"
     CDS             complement(3122..4471)
                     /gene="lysC"
                     /locus_tag="ACHY5W_004102"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFS4448876.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4563..4759)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            4996..6645
                     /gene="pgi"
                     /locus_tag="ACHY5W_004103"
     CDS             4996..6645
                     /gene="pgi"
                     /locus_tag="ACHY5W_004103"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4448877.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1724 a         1638 c         1808 g         1703 t
ORIGIN      
        1 cgccgataaa gatgccgggt aacaggcaaa agggaattcc cctgtcgctt cgtgtctgct
       61 tccgcgacgg ctattaagca aggaatagat aatgattaaa aaaataagcg taagaaaaga
      121 tcagttggca ttactctcca gaaacggcga ttactacaaa gtgctgcatg caggcgagca
      181 tattttaccc tggttaaata ccccggaagt gttgctgatt acgctggatg gtagcgaagt
      241 tcccgacgtg ctggcggatt atttgcgccg ctttcagcct gattgggtgg aaagatattg
      301 cgtggtggca gatttgtcgg agactgaagc tggggcgttg tatatggacg gtattttgtt
      361 ggagatgctg ccgccatcaa cgcgtcgttt gtactggcgt gtagaggatg atttggcgct
      421 ggtacggatg aatacgcagc aagtccaggt acaaacggag gtgatgaatg cggtattgca
      481 gccacggcgt aaaggggctg ttaaagggcg cgatgcgata ctgacggtgc aggttccggc
      541 ctggcatgta ggcgtgctga aaatcgatgg tgaaacgcag gcgctgttac cgccggggct
      601 taccgcgtac tggaaaatta accatctggt tgaagctgaa gtggttgaca cccgcttgca
      661 ggtactggaa gtcagcgggc aagaaatctt aacgaaagat aaggtcaatc tgcggattaa
      721 tctcgccgct aactggcgct atagcgatgt gttgctggca tttagtcagt taacgaagcc
      781 gattgatcat ttgtatcggg aattacaatt tgccctgcgt gaagcggtag gtacgcgcac
      841 actggatgaa ctgctggaag ataaacaggt cattgatgac gttgttagcg agcaggtgaa
      901 attacgcatg aagccgttcg gtatggagat cgcttcgtta ggagtaaaag atatcgtcct
      961 gcctggcgat atgaaaaata ttcttgcgca actggtagag gcggagaaat ccgcgcaggc
     1021 caatgtgatt cgccgacgtg aagaaacggc agcgacgcgc tcgttactga acaccgccaa
     1081 agtcatggag aacaatccgg tagcgttgcg tttaaaagag ctggaaacgc tggagagagt
     1141 ggctgagcgg attgataaca tctctgtatt tggcggtctg gatcaggttc tgcatgggtt
     1201 ggttaatatc aaagggtgag taacatgcca ttaaatggag tcagtgctgc gatgcgcgaa
     1261 agggtatcgc agcagttaaa agaaattgaa cgtcgctatg gcgtgaaagt gctgtacgcc
     1321 tgcgaatcgg ggagtcgcgg ctggggattt gcttcgcccg acagcgatta cgatgtgcgg
     1381 tttttgtatg tccatccgct ggaatggtat ctgcgggtag aggcaccacg cgatgttatc
     1441 gaactgccca tcgacgacga gttagatgtt tccggttggg aatggcgcaa agcgctggga
     1501 ttgctgaaag gtgcgaatcc cacgctaatt gaatggctgg attcaccggt ggtctatcag
     1561 caagatgaag agacgataac ggcgctgaag gcaatggtcc caacgtggtt ttctccgttg
     1621 cgtgcgcgct ggcattacta ttcgatggcg cagaaaaact tccgcggata tttgcagggc
     1681 gatgaagttc gactgaaaaa atacttttac gtgctgcgtc cgttgctggc cgtgcgttgg
     1741 gttgaagcgg gaaaaggcgt accgccaatg cgtttttccg aattgctggc gggaagtgag
     1801 ttggatgcgg ccttacgcgc agaaatagat gaattactgg aacgtaaaca gcgtgcgggt
     1861 gaagcggaat atggcccacg ccgttcattg ctacatgcgt ttatccatgc ggaactggcg
     1921 cgtggggaaa ttccgcctgt gttaccggat agtagagaag gggacgttaa gaaactggat
     1981 agcttactgt accagacggt aatgcgccgg gcgtgatgcc cggcgttatt tcaggtcatc
     2041 agcttttatt attcttgtgc acttccgctg atttcatttt gtatatgcgt tgcaatatcg
     2101 caattggttg ccgcttgtga actaaatatc atcttctcac gcgaatacac cgcaaactta
     2161 tctgccaggt ttgaagcaaa caccttgctg gcattttctg ctagcaaatc ggtaacgata
     2221 tcatggtgat ttttatccac tttgcgggcg atatttcccg cctctttgtc cttattatac
     2281 cgttcgttgg tgctttccca gcttaatgcc gctgaggtgg tttttatcat gacttctgtt
     2341 cggtagcctg catctttgag ttttttaatg tttgtagcgg cgtagcggca ttacgaaatg
     2401 ttccttcgac aacaatattg agtttattgg ctatggccct ctctattgca cgttcgaccg
     2461 tttcgccgga aaattttgcc gtatgggtaa caaaatcgtc gccataagtt tgatatatat
     2521 aattaaaatc agggtgtaga aatctgaaat catcaccatt aataatcatt atattattct
     2581 ttgtttccct ttttgctaat tcgattaggg cggattttcc ggctcctggt tgtccaccta
     2641 aaacaaaacc aatgggactc tgctgggatg tgacgtcatg gtttgtcaga cgctgccaaa
     2701 tagaatcaaa agacgcttta ttttctgaat gcgcagtatt attaaattca cgctgatact
     2761 tttttctgac ctgctggagg cgtaactttc cctgtgtaaa atcaacaagt tctgctggag
     2821 tggaataaag attgtcgcga tagctgacta attgtgatag ctcatcgtct gagtcctgaa
     2881 cttgtgagga aaaacaactg tcgacgatat ttgataaaaa cgcgatgcac tgactggcgc
     2941 cgtttaaaag tgcatccttt tccattaaag tagccattaa cactcctgtt aaataatatt
     3001 tttcggtgat tataatactg ttctacagga gtgattttgc atttcagata ttatttaatc
     3061 tatgtttgca gtgaggatgg cgtataacaa ttggtgagcg ttatacgcca gtaggaaaat
     3121 attactcaaa caaattaaaa tgcagcttct gcaccacctg ctcggcatct tcgccgggca
     3181 ccaggaagca caggttatgg ctggatgcgc cataacaaat catgcgaatg ttgaacggtt
     3241 ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt tgacaggtca ttgccaatca
     3301 acgcgaccag cgccagacct tcttccacct ccacccggca cagtgcggaa agctccatca
     3361 gcagagattg cgtcagcaac gtatcgccgg tggaggttga accggtggta tcaagggtta
     3421 atgccacgct cacttctgat gtggtgatta agtctaccga aatattatgc cgcgcgagga
     3481 tgccgaaaac ttccgcgagg aaaccgcgag aatgcagcat attcaggctg tgcaaagtga
     3541 gcagagtctg attgcgacga agcgccagcg cgcggaacag cggcggattt tcagttttat
     3601 tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga gccgacaaag accgggatat
     3661 cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac ttttgcacca aaagttgcca
     3721 tctctgccgc ttcggcaaac gcgatttcat caatgcgttt tgctgcggaa actacgcgtg
     3781 gatcggtggt gtagataccc gggacgtcgg tccagatatc aacacgagat gcgtgtaaag
     3841 cctccgccag caaggctgcc gtataatcgc tgcctccacg gccaagcgtc gttgtacgac
     3901 ctttattttc gctaccgata aatccctggg tgatcactaa gccttcattg aggcgtggga
     3961 gcagctgcag cgcggccagt tccgccagcg cggctacatc tggctctgca cgaccaaatc
     4021 ggtcgttggt acgcatcact ttacgtacat caaaccactg tgcctgaaca tcgcgttcgc
     4081 gcaggatctc gacaaacagc agggtcgaca tcagctcgcc gtggctgacc agttcatctg
     4141 tcagcgccgg agacgttgcc agcgccgccg cttctgccag aacagtaatg ttctccagca
     4201 gacgttcaat ctcttcacgg ataacgttcg ggtaacgcag acgttccaga atggcaaact
     4261 ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc aggttccagt ccttccgcta
     4321 aagcgaccag cagattagtg ataccagcag aagccgagag gacaactaaa cgcacgttgg
     4381 catcagaaag cacaatatca gcgctgcggt tcatggcgtc aaaatcagct acgctggtac
     4441 cgccaaattt ggagacaaca atttcagaca taactacctc gtgtcagggg atccattttc
     4501 agccttggca caagggaaga gcggaagacg ggtgggcgca gagcgatact tcgctactat
     4561 tttcacccag aagtgctcca ccacttgcga aacgcccgac tgcgaacgct tctggtgaca
     4621 acccagggga ttcagcccct gtagccgatg atgaacgtgg ccagccgttc aatcacctcg
     4681 gcgatgcacc ccctcaggtg ttatcacagg actggctcct ccaacaccgt tacttgggca
     4741 acgcgcctct tctggcctgc gctagcgcag gtagtacatt tataaataaa gggtgagcgg
     4801 ggcggttgtc aacgacgggg tcatgcggat ttttcatcca ctcctggcgg tcagtagttc
     4861 agctaataaa tgcttcactg cgctaagggt ttacactcaa cattacgcta acggcactaa
     4921 aaccatcaca tttttctgtg actggcgcta caatcttcca aagtcacaat tctcaaaatc
     4981 agaagagtat tgctaatgaa aaacatcaat ccaacgcaga ccgctgcctg gcaggcacta
     5041 cagaaacact tcgatgaaat gaaagacgtt acgatcgccg atctttttgc taaagacggc
     5101 gatcgttttt ctaagttctc cgcaaccttc gacgatcaga tgctggtgga ttactccaaa
     5161 aaccgcatca ctgaagagac gctggcgaaa ttacaagatc tggcgaaaga gtgcgatctg
     5221 gcgggcgcga ttaagtcgat gttctctggc gagaagatca accgcactga aaaccgcgcc
     5281 gtgctgcacg tagcgctgcg taaccgtagc aataccccga ttctggttga tggcaaagac
     5341 gtaatgccgg aagtcaacgc ggtgctggag aagatgaaaa ccttctcgga agcgattatc
     5401 tccggtgagt ggaagggtta taccggcaaa gcaatcactg acgtagtgaa catcgggatc
     5461 ggcggttctg acctcggccc gtacatggtg accgaagcac tgcgtccgta caaaaaccac
     5521 ctgaacatgc actttgtgtc taacgtcgat ggcacccaca tcgcggaagt gctgaaaaaa
     5581 gtaaacccgg aaaccacgct gttcctggta gcatctaaaa ccttcaccac tcaggaaacc
     5641 atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag cggcaggtga tgaaaaacac
     5701 gttgcgaaac actttgcggc gctctcaacc aatgccaaag ccgttggcga gtttggtatt
     5761 gatactgcca acatgttcga gttctgggac tgggtcggcg gtcgttactc tttgtggtca
     5821 gcgattggcc tgtcgattgt tctctccatc ggctttgata acttcgttga actgctttct
     5881 ggcgcacacg cgatggacaa gcatttctcc accacgcctg ccgagaaaaa cctgcctgta
     5941 ctgttggcgc tgattggcat ctggtacaac aatttctttg gtgcggaaac tgaagcgatt
     6001 ctgccgtatg accagtatat gcaccgtttc gcggcgtact tccagcaggg caatatggag
     6061 tccaacggta agtatgttga ccgtaacggt aacgttgtgg attaccagac tggcccgatt
     6121 atctggggtg aaccaggcac taacggtcag cacgcgttct accagctgat ccaccaggga
     6181 accaaaatgg taccgtgcga tttcatcgcc ccagctatca cccataaccc gctctctgat
     6241 caccaccaga aactgctgtc taacttcttc gcccagaccg aagcgctggc gtttggtaaa
     6301 tcccgcgaag tggttgagca ggaatatcgc gatcagggta aagatccggc aacgcttgac
     6361 tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga ccaactccat cctgctgcgt
     6421 gaaatcactc cgttcagcct gggtgcgttg attgcgctgt atgagcacaa aatctttact
     6481 cagggcgtga tcctgaacat cttcaccttc gaccagtggg gcgtggaact gggtaaacag
     6541 ctggcgaacc gtattctgcc agagctgaaa gatgataaag aaatcagcag ccacgatagc
     6601 tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg gttaatcatc gttcatgcct
     6661 gatgccgcta tgtaggccgg ataaggcgtt cacgccgcat ccggcaaccg atgcctgatg
     6721 cgacgcggtc gcgtcttatc aggcctacag gtcgatgccg atatgtacat cgtattcggc
     6781 aattaataca tagcacgatt gattaaataa ccttaataac aatgccgacg ttatgtcggc
     6841 attgttttat cagataaatc cgcttgtcta tga
//
LOCUS       DAZUGK010000225         6765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZUGK010000225 DAZUGK010000000
VERSION     DAZUGK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.226"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            283..1815
                     /gene="ycgB"
                     /locus_tag="ACHY5W_004104"
     CDS             283..1815
                     /gene="ycgB"
                     /locus_tag="ACHY5W_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFS4448878.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(1867..2586)
                     /gene="fadR"
                     /locus_tag="ACHY5W_004105"
     CDS             complement(1867..2586)
                     /gene="fadR"
                     /locus_tag="ACHY5W_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFS4448879.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2808..4349
                     /gene="nhaB"
                     /locus_tag="ACHY5W_004106"
     CDS             2808..4349
                     /gene="nhaB"
                     /locus_tag="ACHY5W_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFS4448880.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4495..5025
                     /gene="dsbB"
                     /locus_tag="ACHY5W_004107"
     CDS             4495..5025
                     /gene="dsbB"
                     /locus_tag="ACHY5W_004107"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFS4448881.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5071..6339)
                     /gene="umuC"
                     /locus_tag="ACHY5W_004108"
     CDS             complement(5071..6339)
                     /gene="umuC"
                     /locus_tag="ACHY5W_004108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFS4448882.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QADHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6339..6758)
                     /gene="umuD"
                     /locus_tag="ACHY5W_004109"
     CDS             complement(6339..6758)
                     /gene="umuD"
                     /locus_tag="ACHY5W_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS4448883.1"
                     /translation="MLFIKPADLREILTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         1615 a         1718 c         1672 g         1760 t
ORIGIN      
        1 acatcttgat aactcagttg aaaatattat tcaacatacg gcttttttat ggtgagctgg
       61 ctcacatctc cctgactctg cggcaatatg gcgtctttag tatctcctgc tgaacgccct
      121 ttttatccag cataaaattc cgttcagaag cggattagtg gcactctgat aataagaaat
      181 cgcaaagaaa aaaattctcc ggcgacgcgg tttttaacag gtgttctatg cttgaaatga
      241 ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc gtatggcgac gatcgattct
      301 atgaataagg acaccacacg tttgagcgat ggacccgact ggacgttcga cctgctggat
      361 gtttatctgg cagagataga ccgggtggcg aaactctacc ggctggatac ctacccgcac
      421 cagattgaag tgataacctc agaacagatg atggatgcct actccagcgt cggcatgcca
      481 attaactatc cgcactggtc attcggtaaa aagtttatcg agactgaacg gctgtataag
      541 cacggtcagc aaggactggc ctatgaaatc gtcattaact ctaacccgtg tatcgcttac
      601 ctgatggaag agaacaccat taccatgcaa gcgctggtga tggctcatgc ctgctatggg
      661 cataactctt tcttcaaaaa caattactta ttccgtagct ggaccgacgc cagttcgatt
      721 gtcgattatc tgatttttgc ccgtaaatat attaccgagt gcgaagagcg ctatggcgtt
      781 gatgaagtag aacggcttct ggactcgtgc cacgcgctga tgaactacgg cgtggaccgg
      841 tacaaacgcc cgcaaaaaat ctcgctgcaa gaagagaaag cccggcagaa aagtcgcgaa
      901 gagtatctgc aaagtcaggt caatatgctc tggcgtaccc tgccgaagcg cgaggaagag
      961 aaaacggttg ctgaagcgcg ccgctatccg tccgagccac aagaaaacct gctctatttt
     1021 atggagaaaa atgcgccact gctggaatca tggcagcgtg aaatcctgcg tattgtgcgt
     1081 aaggtgagcc agtattttta tccgcaaaaa cagactcagg tgatgaacga aggctgggcg
     1141 accttctggc actacaccat ccttaaccat ctgtatgatg aagggaaagt aacggaacgt
     1201 tttatgctgg agtttttgca cagccacacc aatgtggtct tccagccgcc ctataacagc
     1261 ccgtggtaca gcggcatcaa cccgtatgcc ctcgggttcg ccatgttcca ggatattaaa
     1321 cggatttgtc agtcgccaac ggaagaagac aaatactggt tcccggatat cgccggttcc
     1381 gactggctgg aaacgctgca tttcgcgatg cgtgatttca aagatgagag ttttatcagc
     1441 cagttcctgt caccgaaagt gatgcgtgat ttccgcttct tcaccgtgct ggatgatgat
     1501 cggcataatt atctggagat ttccgctatt cataatgaag agggttatcg ggagatccgt
     1561 aaccggttat cgtcgcaata taacttaagt aatctggagc cgaatattca gatctggaac
     1621 gtggatttgc gcggcgaccg ttcgctgaca ctgcgttaca ttccacataa tcgcgcaccg
     1681 ctggatcggg ggcgcaaaga agtactgaag catgtgcatc gcctgtgggg atttgatgtg
     1741 atgctggaac agcaaaacga agacggcagc gtcgagttgc tggaacgttg cccgccgaga
     1801 atgggaaatc tgtaaaaaca acaaaaaacc cctcgtttga ggggtttgtt ttttaaacgg
     1861 aagggattat cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca
     1921 aatctcgcca ctctcatgcc catagcgacg cactgtttcg tacacctgat cgtgcgcgcc
     1981 ttcactgcac aacgccgaca gtttgtggta gaagcccagc gccagactgc gcgcttccgg
     2041 attggcgaaa tagtgacgac caatgcgcgt atacagtcct ttcatcccgt taaggatcag
     2101 accgtaaatc gggttgccgg aagcaaacgc cagaccgcgg aatatgttgt aatccagctc
     2161 ggcaaaggcg tcagcgtgat cggccacttc attagcggta gccagcactt cctgcgcttt
     2221 atcgggatgc tgacgaaacg cggtgcgaat aaaaatagtt gaaatattgg tacgcaccga
     2281 cagcaaatta tcaataagct gcggcacact ttcgtgatcc agtcgcgcca gtgtttcaag
     2341 gatatttaaa ccagaggttt cccagaaatt attcaccttc gtcggcttgc catgttgaat
     2401 ggtcaaccag ccatctcgtg ccagacgctg taacacttca cgtaacgtag tacgcgttac
     2461 gccaattaat tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt
     2521 attccagata ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat
     2581 gaccatagtg agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc
     2641 taactacgct gatagcagac aaaaagaaga aaaaagggat caaggcttca ttttccgcat
     2701 ctctttcccc ttgactatca agacttgcct cactcctgcc tgaccgttaa gctttgcgtt
     2761 taaacataaa aacatcattg attatttgta aggtaaggga aaccatcatg gagatctcct
     2821 ggggccgcgc gctatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc
     2881 tcatcatttt cttaatcgta aatccgttaa ttttcctcat cagccctttc gtcgctggct
     2941 ggttgctggt agcggaattt attttcactc tggcgatggc cctgaaatgc tacccgctac
     3001 tccccggtgg tctgttggct atcgaagcgg tattcatcgg catgaccagc gcggaacacg
     3061 tccgtgaaga ggtggcggca aatcttgaag tcttgctgtt attgatgttt atggtggcgg
     3121 gtatctattt tatgaaacag ctgttgctgt tcatatttac ccgtttgctg ttaagcattc
     3181 gctccaaaat gctgctgtcg ctctcttttt gcgtggcggc agcgttcctc tccgcgttcc
     3241 tcgatgcctt aaccgtcgtg gcggtggtga tcagcgttgc agtcggtttt tatggtattt
     3301 atcatcgcgt agcctcttcc cgtaccgaag acaccgacct gcaagacgat agtcatatcg
     3361 acaagcatta caaagtggtt ctggaacagt tccgtggctt tctgcgtagc ctgatgatgc
     3421 atgccggtgt cggcaccgca ttaggcggcg taatgaccat ggtgggcgaa ccacagaacc
     3481 tgatcatcgc taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccgg
     3541 tgaccgttcc ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt
     3601 ggtttggcta cggtgaaacg ctgccggaga aagtccgcga agtgttgcaa cagtttgacg
     3661 atcaaagccg ccaccagcgt acccgtcagg ataaaatccg tctgattgtc caggcgatta
     3721 ttggcgtctg gctggtgact gcgctggcgt tgcatctggc ggaagttggt ttgattggtt
     3781 tgtcagtcat tattctggcg acatcattga ccggtgtcac cgatgaacat gctatcggta
     3841 aagccttcac cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcggtagtcg
     3901 cggtgattat cgaccaacaa ctgttttcgc cgattattca gtttgtgttg caggcatcgg
     3961 aacatgctca gctgtcgctg ttctatattt tcaacggtct gctgtcatcc atttcggata
     4021 acgtcttcgt ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta
     4081 tcacgttgaa gcaatacgag ctgctggcgg tcgccattaa taccggtacc aatctgccct
     4141 ctgtcgctac gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc
     4201 cattgattcg cctctcttat ggccgcatgg tgtggatggc cctgccttac accctcgtcc
     4261 tgacactcgt cggactgctc tgcgtcgagt ttacgcttgc ccctgtaacc gaatggttta
     4321 tgcaaatggg ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt
     4381 ttgccttttc gcccgataat tgtccaattg cgtcctattt ttgctgcctc ctggtggcgc
     4441 agcgaatgaa ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg
     4501 cgatttttga accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttactgct
     4561 ctggcactgg aactgacggc gctgtggttc cagcatgtga tgttactgaa accttgcgtg
     4621 ctctgtattt atgaacgctg cgcattattc ggcgttctgg gtgctgcgct gattggcgcg
     4681 atcgccccaa aaacgccact gcgttatgta gcgatggtta tctggttgta tagtgcgttc
     4741 cgcggtgtgc agttaactta cgagcacacc atgcttcagc tctatccttc gccatttgcc
     4801 acctgtgatt ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa
     4861 gtgtttgtcg cctctggcga ttgcgccgag cgtcagtggg attttttagg tctggaaatg
     4921 ccgcagtggc tgctcggtat ttttatcgct tacctgattg tcgcagtgct ggtggtgatt
     4981 tcccagccgt ttaaagcgaa aaaacgtgat ctgttcggtc gctaatccat tcggcgctcc
     5041 tgcgggagcg cttttttcct gccgctatat ttatttgacc cgcagtaaat cagaacttcg
     5101 cgttgtataa cgtggtgaaa gcatggctcg cttcatctgc cattgttgct ggatcccctg
     5161 cccggcaaaa tagagtgttc ctctgccctc tttagcattc agtgtatcca ttaccgccat
     5221 caattgctca ctcccggggc gcggagcgtt gtcatcgaat aaattgagct gcgcgactcc
     5281 ctgactgaag aaatccccca gcatcacgcc cgctttttgg tagcgatggt ccgcttgcca
     5341 gatggcatcc agagatcgcg tagcggcgtt aatgatatcc ctgctgtcct gagtgggcgt
     5401 cagcagtttt accgacgcac tattgccgta ataaggttca ttgagcgcaa atggtgacgt
     5461 cttaataaat gtggagataa accgacaata ttgatgctca ctgcgaagtt tttccgccgc
     5521 ccgggcagcg taactacaaa tggcctgccg catcgacgta taatccgtga tgcgttcacc
     5581 aaacgagcgg gaacagataa tttcctgctt tgtcggtgca aattcttcca gttgcaaaca
     5641 gggttcaccg cgcagttcac gcaccgttct ttcgagcacg acattaaaat gtttacggat
     5701 aaaccggata tctgtatccg ccaaatcgag aacagttttg atccccatcg cgtccagttt
     5761 tttgctgatc cgccgtccaa tcccccagac gtcatccacg gggagagcag acattaattt
     5821 tcgctggcgt tccagatttg ataaatccac caccccaccc gtctgccgct gccatttttt
     5881 tgccgcatga ttggcaagct tagccagcgt tttggtctgg gcgatcccca caccaacagt
     5941 aagatgggta cgttgtagca ccgttgcgcg aatttctctg ccaaagtcag tcagatcgcg
     6001 acaattacgc acacctgtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg
     6061 gggcgatagt tcttccagcg tcgacatcac ccgattgctc atgtctgcgt aaagctcata
     6121 attgctgcta aagcaaacca cgccacagcg acgaaacaga tctttttgtt tgaaccaggg
     6181 atcgcccatt ttaacgccaa gcgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc
     6241 attattcgat agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca
     6301 gctggcataa aacgcgttta catcacagag ggcaaacatc agcgcatcgc cttaacgacg
     6361 tggatcacca caccaaagac atccagcgta tcttcactac tgatggtaat gggcgagtac
     6421 gcgctgttca tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt caccgtaaac
     6481 tcgccgtcaa cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca
     6541 atcagtaaat caccgtcact aattccacca tcaatcatag aatcaccact tgctttgacg
     6601 aagtaagtcg cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcaacg
     6661 taatctgcgg ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc
     6721 aaaatttcgc ggagatccgc aggcttgata aacaacataa taatc
//
LOCUS       DAZUGK010000226         6518 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZUGK010000226 DAZUGK010000000
VERSION     DAZUGK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.227"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..376
                     /gene="yjbE"
                     /locus_tag="ACHY5W_004110"
     CDS             134..376
                     /gene="yjbE"
                     /locus_tag="ACHY5W_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFS4448884.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            563..1201
                     /locus_tag="ACHY5W_004111"
     CDS             563..1201
                     /locus_tag="ACHY5W_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFS4448885.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTNQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1198..1935
                     /locus_tag="ACHY5W_004112"
     CDS             1198..1935
                     /locus_tag="ACHY5W_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS4448886.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1935..4031
                     /locus_tag="ACHY5W_004113"
     CDS             1935..4031
                     /locus_tag="ACHY5W_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS4448887.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSHEGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4078..4356)
                     /locus_tag="ACHY5W_004114"
     CDS             complement(4078..4356)
                     /locus_tag="ACHY5W_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448888.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4357..4500
                     /locus_tag="ACHY5W_004115"
     CDS             4357..4500
                     /locus_tag="ACHY5W_004115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448889.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4626..5036
                     /gene="psiE"
                     /locus_tag="ACHY5W_004116"
     CDS             4626..5036
                     /gene="psiE"
                     /locus_tag="ACHY5W_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFS4448890.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFASEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5080..>6518)
                     /gene="xylE"
                     /locus_tag="ACHY5W_004117"
     CDS             complement(5080..>6518)
                     /gene="xylE"
                     /locus_tag="ACHY5W_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFS4448891.1"
                     /translation="TLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLL
                     GFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPD
                     NTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIF
                     GQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRG
                     KQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQ
                     FVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQII
                     GALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRG
                     KALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFV
                     PETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1606 a         1672 c         1780 g         1460 t
ORIGIN      
        1 attcattcca attatcctaa aaccccatcg ctaattcccc gtgccgtaat tcgcatgctt
       61 tagttgtgta tactcgatcc cgcccgaaat gtttttgggt aaatctccat tcattcaatg
      121 aagggaaatt gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc
      181 gacttctgcg tgggctgcac ctgtacaggt gggcgaagcg gcagggtcgg cagcaacgtc
      241 ggtttcggcg gggagttcct ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt
      301 cgcgctcgcg gcaaccggtg gcggtgatgg ttctaatacc gggaccacaa caaccacgac
      361 caccagtacc cagtaataaa gtatgtatcc ccaaaataat tcgagttgca ggaaggcgac
      421 aagcgagtga gtccccggga gcatagataa ctatgtgacc ggggtgaacg agtgcggtca
      481 ccgcatctgc agcttgaagt atgaagggga ttaaccataa ccacactccg gtgtggttat
      541 tctgcccctc tggagaagag tcgtgaagcg acctgcattc attcttatct gcctgctatt
      601 acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca gtctgttcgg
      661 tacgccaggc gtacagctga cggatgatga tattcaaaat atgccctacg ccagccagta
      721 catgcagctt aatggcgggc cgcagttatt tgtggtgctg gccttcgctg aagacggaca
      781 acaaaaatgg gtcactcagg atcaggccac tctcgtcaca caacatggtc gtctggtgaa
      841 gactctgctt ggcggcgaca acttgattga ggtgaataac cttgccgccg acccgctgat
      901 taaacccgca caaatcattg atggtgcaac ctggacgcgc acgatgggct ggaccgaata
      961 ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca ccgataccgt
     1021 caaagtcggc agcgatgaaa ccccggttcg cgtgctggac gaagaagtct ccaccaacca
     1081 ggcgcgctgg cataaccgct attggatcga cagcgaaggg caaattcgcc agtcggaaca
     1141 gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg caaaacaatg
     1201 attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt cttcgcggca
     1261 ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac gggcgcagag
     1321 catttaatcg atctggtagg ccaaccgcgg ctggcaaaca gctggtggcc tggtgcggtg
     1381 attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt gctgacacgg
     1441 ctggcagaac agggcgcaga ttccagcgcc gacgatgccg ctgcgattaa cgctttacgc
     1501 cagcaaattc aggcgttgaa agtgacgggt aggcaaaaaa tcaatcttga tcccgatatc
     1561 gtccgcgttg ccgaacgcgg taacccaccg ttgcagggca actacacgct gtgggtcgga
     1621 ccaccgccgt ccacggtcac gctgttcggg cttatcagcc gtcctggcaa gcagccattc
     1681 actccaggtc gcgacgtggc gagctatctc tccggccaga acctgcttag cggtgcggat
     1741 cgcagctacg cgtgggtggt ttacccggac ggacgcacgc aaaaagcgcc ggtggcttac
     1801 tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg cctcgcagac
     1861 tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac tctgacgcag
     1921 cggatacctc aataatgaaa aaaagacatc tgcttagctt actggcgctg ggcattagca
     1981 cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg gatttcggtg
     2041 gcgtaggatt attacaaacg cccacggctc gcatggcgcg ggaaggggag ttgagtctga
     2101 actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc ttcccgtggc
     2161 tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc gtcgaagcgt
     2221 tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt ttgtgggaag
     2281 agagttattg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt acggggctgt
     2341 ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt acgctcggcc
     2401 tgggctgggg atatttgggc accagcggta atgtgaaaaa tccgctctgt tcagccagtg
     2461 ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac ggtagccaga
     2521 tgttccacgg tcctgcctca ctgtttggcg gtgtggaata ccagacgccc tggcaaccgc
     2581 tgcgcctgaa actggagtat gaaggcaata attatcagca ggattttgcc gggaagctgg
     2641 agcaaaaaag taagtttaac gtcggtgcga tttatcgcgt taccgattgg gccgacgtta
     2701 accttagcta tgaacgtggc aacaccttta tgtttggcgt tacgttgcgc accaacttta
     2761 acgatctgcg cccgtcttac aacgataacg cccgcccgca atatcaaccg cagccgcagg
     2821 atgccatttt gcagcattcg gtggtggcga atcagttaac gctgttgaaa tacaatgccg
     2881 gacttgccga tccacagatc caggcgaaag gcgatacgct gtatgttacc ggcgagcagg
     2941 tgaaatatcg tgattcgcac gaagggatca tccgtgctaa tcggatcgtg atgaacgatc
     3001 tgccggatgg gatcaagacg atccgcgtta cggaaaatcg ccttaacatg ccgcaggtga
     3061 cgacggaaac ggatgtcgcc agcctgaaaa atcatctcgc cggagagccg ttgggccacg
     3121 aaacgaagct ggcgcaaaaa cgcgtcgagc cagtggttcc gcagtccacc gagcagggct
     3181 ggtatatcga caaatcgcgc tttgatttcc atatcgatcc ggtgctgaac cagtcggtcg
     3241 gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca gatttgtggc
     3301 tgacggacca tctgctgacc accggcagcc tgtttgcaaa tcttgccaac aactacgaca
     3361 agtttaacta caccaatccg ccgcaggact cgcacttacc gcgcgtgcgt acccatgtgc
     3421 gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac ttccagcatc
     3481 tgggcaacgg cttctacggt caggtctacg gcggctatct cgaaaccatg tttggcggtg
     3541 cgggggctga ggtgttgtat cgcccgctgg acagcaactg ggcgtttggt ctggatgcca
     3601 actacgttaa acagcgcgac tggcgtagcg cgaaagatat gatgaaattc accgattaca
     3661 gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgctcag gatgtgttag
     3721 ttaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg gagatcgcca
     3781 aacgctttga cagtggtgtg gtggtgggtg gctatgccac gatcacgaat gtttcgaaag
     3841 aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg ttggatctct
     3901 tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg acgcgtgacg
     3961 gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac cgtagcgtca
     4021 atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc ttcattctta
     4081 atggcctgtc gagccatcag gaacgcagtt cacccccggt ttgcgatctg gtgtgtaaag
     4141 gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt gcctgagcga
     4201 ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat cagcagtacc
     4261 gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag caaaataagg
     4321 cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa cattgtcgat
     4381 ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga attaccggct
     4441 ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc ctacgggtag
     4501 gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac atacacaaaa
     4561 aatatagatc tccgccacat ttttgcgtta tacaggaagc tcgccactgt gaaggaggta
     4621 ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt ttgcagaccg
     4681 tactcaatct tggcctgttg tgcctcggcc tgattttggt tgtcttcctc ggcaaagaaa
     4741 cggtgcatct ggctgatgtg ctgttcgcgt cagaacaaac cagcaaatat gagctggtag
     4801 aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg aagtactttc
     4861 agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc gccattgtgc
     4921 gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg gctgcgatcc
     4981 tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg gagtaaaaag
     5041 acagcacgcc gaactggcgc ggcgtgctgg acaggaagat tacagcgtag cagtttgttg
     5101 tgttttcttc gtttccggtt cccagagggc ttccagctcc tcaagggttt tacctttggt
     5161 ttccgggaca aatttccaca taaatagtgc tgccagaacg cccatacacc cgtaaatcca
     5221 gtaggagaaa ccgttgtgga aatgggctac cagccaggag tttttgtcca tcatcgggaa
     5281 ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg ccagcgcttt
     5341 accacgaatg gcattcggga agatttccga cagcagaacc cagcataccg gaccccagga
     5401 catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac ccggtgcctg
     5461 agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagcg cgccgataat
     5521 ttgcagtggc ttacgaccaa atttatccac cgtcataatt gccagaacgg tgaaggtgag
     5581 gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg cccccagcgt
     5641 tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt gctggaagat
     5701 ggagagcata acgccgatga caatcacgcc cacgccaaac atcagcagac gaccaccggt
     5761 tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg caagggtgtt
     5821 gcccataatt ttgcgcagga taccttccgc ctgttcttgc ttgccgcgcg acatcagcca
     5881 gcgaggactt tctggcacgg tatacaacag cattaagaag agtagtgcag ggatacattc
     5941 cgaggcaaac atataacgcc agccgtcagt attcagccag ctggcatcac cggaacgcgc
     6001 aataaaatag ttcacgcagt aaactaaaag ttgcccgaaa ataatcgcaa actggttaaa
     6061 agagaccagt ttcccgcgaa tatgagcagg agcaagttcc gcaatataca ttggtgaaag
     6121 cattgaggct aaaccaacgc caataccgcc aataatgcga taaataacaa attccgggac
     6181 ataacctgcc agataaacag gcactgtgtt gtccgggttt atagaggtaa aaccaagttc
     6241 tggccaggca gaacctacac cagaaataaa aaacaggaca gcagcaatct taagtgaatc
     6301 acgacgaccg aagcggctac tgcaataacc accgagggca ccaccgatga tgcacccaat
     6361 cagagcgctg gctacgcaaa accctaacag ggagttggca gcggattcac ttaagttttg
     6421 tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg cggtgtcgta
     6481 gccaaataat aaaccaccta atgtagcgac taaggtaa
//
LOCUS       DAZUGK010000227         6487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZUGK010000227 DAZUGK010000000
VERSION     DAZUGK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.228"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(86..268)
                     /locus_tag="ACHY5W_004118"
     CDS             complement(86..268)
                     /locus_tag="ACHY5W_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448892.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            597..1469
                     /locus_tag="ACHY5W_004119"
     CDS             597..1469
                     /locus_tag="ACHY5W_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS4448893.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRVLSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1841..4690
                     /gene="cah"
                     /locus_tag="ACHY5W_004120"
     CDS             1841..4690
                     /gene="cah"
                     /locus_tag="ACHY5W_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFS4448894.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4801..>6487
                     /locus_tag="ACHY5W_004121"
     CDS             4801..>6487
                     /locus_tag="ACHY5W_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFS4448895.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1636 a         1633 c         1803 g         1415 t
ORIGIN      
        1 attaaataaa atattgagcg cctgattata tttattcatt cattaatatc tgttattgat
       61 tggcttacta tcagctcccc ccgtattaat cagagctttc atttattttt agtgcataat
      121 cagatgccgc ctcatcatta tcctcactat tatcatagcc gaaaacttcc cccggacacg
      181 ggcgttcacg ataatagtca tcgtcatagt tttcatcgtc agataatgat atgtcgtatt
      241 ctgaatatga ttcaccacaa ttatacatga aatcatctcc tttggggggg gcaataatta
      301 gtgagtttat tcgaaaaaat gatgagcgcc agaccaatcc acccaacttt tgattataat
      361 catgaagata cttgtctcag tacaaattga atcaaactca atgcagcatg attcaccttt
      421 atattctctc agggaaacca aataccacta ctcaactatt aagaacaaaa ttcgggatgt
      481 atgattatca gaagatacct gcatcctaaa taatttcaga cagatattat cgttgtgaat
      541 cccccgccac ctttccggtg cgcggggttt tgtctttttt caccgggaat acatgtatga
      601 atccgtctga tgccattgag gcaattgaaa aaccgctctc ctccctgcct tactcgcttt
      661 cccgtcacat cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg attggcatta
      721 tgggtaaaag cggggccggt aaatcctcac tctgtaatgc actgtttcag ggggaggtca
      781 ccccggtcag tgatgttcac gccggcaccc gggaagtgcg gcgcttccgt ctgagtggcc
      841 atggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg gacagggatg
      901 cagagtatga agccctgtac cgtgacattc tgcctgaact ggacttggta ctgtggctga
      961 ttaaagccga tgaccgtgtc ctgtctgtgg atgagtattt ctggcgacac atactgcacc
     1021 ggggacatca gcaggtgctg tttgtggtga cgcaggccga caaaacagag ccctgccatg
     1081 aatgggatat ggcaggtatt cagccttctc ccgcacagga acagaacatt cgcgaaaaaa
     1141 cagatgcggt attccggctg ttccggcccg tacatccggt tgttgccgta tcggcccgca
     1201 ccggctggga actggatacg ctggtcagtg cgctcatgac agcgcttccc gaccatgccg
     1261 ccagtcccct gatgacccgc ctgcaggacg agctgtgcac ggaatctgtc cgggggcagg
     1321 cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacggtggag agcgtctgtg
     1381 ttgcctctgt tgcacgcaca gttctgcgtg ccgtccgtga cacggtggtc tctgttgccc
     1441 gcgcggtatg gaactggatt ttcttctgaa cagtctgcca ggggaaatgt ctgattacat
     1501 tgcgttaacc aactgaaaac atgatacaaa cacctcatgc ccctcctgtt tctcttgcgt
     1561 gactgctcta ctgttaatag aataaaacga tcgataaaac aggtatcgat cgtttatatc
     1621 gatcgtttat agtggttaat ggcctgcgaa aaggcagctg aatctcttca tcatgcagaa
     1681 cggaattgca cacaacagac tgatacttct ctgtctgatg aagggggaga gcgtttacgc
     1741 ccgggacgta tcgctgtgcc cgataactct gttttgtacc tgccggtatc cacttttgtg
     1801 ggtaccggct tttttattca ccctctgtaa ggaaaagctg atgaaacgac atctgaacac
     1861 cagctacagg ctggtatgga atcacattac gggcaccctg gtggtggcct ccgaactggc
     1921 ccgctcacgg ggaaaacgcg ccggtgtggc ggttgcgctg tctcttgctg ctgtcacatc
     1981 agtcccggca ctggctgctg acaaggttgt acaggcggga gaaaccgtga acgatggaac
     2041 actgacaaat catgacaacc agattgtctt cggtacggcc aacggaatga ccatcagtac
     2101 cgggctggaa ctggggccgg acagtgaaga aaacaccggt gggcaatgga tacagaatgg
     2161 cgggatagcc ggaaacacca ctgtcaccac aaatggtcgt caggtcgtgc tggagggggg
     2221 gacagccagt gatacggtta ttcgtgacgg cgggggacag agcctgaacg gactggcggt
     2281 gaacaccaca ctgaataaca gaggcgagca gtgggtgcat gagggcgggg ttgccaccgg
     2341 tacaattatc aaccgcgacg gttaccagag cgttaaaagt ggcgggctgg caacaggaac
     2401 catcatcaac accggcgcag aaggcggccc tgattctgac aactcgtata cgggtcagaa
     2461 ggtccaggga acagcagaat ccaccaccat caacaaaaat ggacggcaga ttatcttatt
     2521 ttccgggcta gcccgtgaca ctctcattta cgcaggtggt gaccagtcgg tacacggaag
     2581 ggccctgaat accacactga atggcggtta ccagtatgtg cacagggacg gacttgcgct
     2641 gaacacggta attaacgagg ggggctggca ggttgttaag gcaggtggcg ctgccggtaa
     2701 caccaccata aatcagaacg gtgaactgag ggtacatgcc ggcggggaag ccactgcagt
     2761 cacccagaac acgggcggtg cactggttac cagtactgct gcaactgtca tcggcacaaa
     2821 ccgtctgggg aatttcacgg tggaaaacgg taaggctgac ggtgttgttc tggaatccgg
     2881 cggtcgtctg gatgtactgg agagccattc agcacagaat accctagtgg atgacggcgg
     2941 taccctggca gtgtctgccg gcggtaaggc gacaagtgtc accataacat ccggtggtgc
     3001 cctgattgca gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat
     3061 tgatggcaca tccggtcagg ccagcggcct gctgctggaa aatggcggca gctttacggt
     3121 taatgccggg ggacaggctg gcaacaccac tgtcggacat cgtggaacac tgacgctggc
     3181 tgccggggga agtctgagtg gcagaacaca gctcagtaaa ggcgccagta tggtactgaa
     3241 tggtgatgtg gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca
     3301 gacgacaccg aatgccgcgc tgagccgtgc tgttgcaaaa agtaactccc cggtaacgtt
     3361 ccataaactg accaccacga acctcaccgg ccagggcggc accatcaata tgcgtgttcg
     3421 ccttgatggc agcaatgcct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa
     3481 aacctggctt gcgtttacaa atgtcggaaa cagcaacctc ggggtggcaa ccaccggaca
     3541 gggtatccgg gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct
     3601 gagtcgcccg cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga
     3661 ctggtacctg cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgtt
     3721 gacacaggca atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt
     3781 aaacggtgaa aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa
     3841 caacggcggt attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg
     3901 tctggagggt gacctgctca gaacagaggt tgccggtatg tctctgacga caggggtgta
     3961 tggtgctgca ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac
     4021 ggtccgggat gatgccggca gtctgggcgg atacctgaat ctggtacaca catcctccgg
     4081 cctgtgggct gacattgtgg cccagggaac ccgtcacagc atgaaagcgt catcggacaa
     4141 taacgacttc cgcgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt
     4201 cagtatcact gacaatctga tgctggagcc acaactgcag tacacctggc agggactctc
     4261 cctggatgac ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca
     4321 tgtgcgtgcc ggtttccgtc tgggcagcca caacgatatg acctttggtg aaggcacctc
     4381 atcccgtgac accctgcgcg acagtgcaaa acacagtgtg agtgaactgc cggtgaactg
     4441 gtgggtacag ccttctgtta tccgcacctt cagctcccgg ggtgacatga gcatggggac
     4501 agccgcagcc ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct
     4561 gcaggccgga ctggaagccc gtatccggga aaatatcacc ctgggcgttc aggccggtta
     4621 tgcccacagc gtcagcggca gcagcgctga aggctataac ggtcaggcta cgctgaatat
     4681 gactttctga ttatacggta tcccggaaat acgatttcag gaaaattcat gcagtaatgg
     4741 gaaaggacgc tcattgctgt tcaccgcagt tctgaattat tgaatttata aaggtgaatg
     4801 atgcacgaaa agaacatcgc cctgctttgt gatgaagccg accggctttt gcaactgaat
     4861 atcaatctgc tccggcaaat ggttgatgag ccggatgtgt tatctgacag taagaacgaa
     4921 aacgggctgc tttttgataa acggaaagcg ctgaaaagaa ttgaggagct ggagggcgaa
     4981 caaatcaaaa ccgcccgcag ggagatggtg ctggctgtgg tcggcacgat gaaggcgggc
     5041 aaatcaacca ccatcaacgc cattgtgggg caggaaattc tgcccaaccg taaccgcccc
     5101 atgacctcgg taccgacatt gatccgccac gttcccggaa aaacggaacc ggtcctccat
     5161 ctggaacata ttcagcccgt ccgcaattta ttaatcacac tgcaggaaaa actcgccacc
     5221 ccggcagggc agcaagtcgc acagtccctg cagcaaaccg gggatacccg cgaactactg
     5281 gatattctgg cggatgatgt ctggcttaaa aatgaatacc acggggaaga cgaaatcttt
     5341 accggactgg catcgttaaa tgacctggtt cgtcttgctg cggcaatggg gactgaattt
     5401 ccttttgacg aatacgcaga agtgcagaaa ctaccggtga tcgacgtggc gttcagccac
     5461 ctggtgggaa tggatgcatg tcagggaaca ctcacactgc tggatacccc cggccctaat
     5521 gaagccggac aaccacagat ggaaatgatg atgcgggatc aactacagaa agcctctgcg
     5581 gtgctggcgg tgatggatta cacccagatg aactcgaaag ccgatgagga ggttcgtaaa
     5641 gagcttaatg ccattgctga cgtctcagcc ggtcgtctgt ttgtgctggt caataaattc
     5701 gatgaaaaag atcgcaatgg tgatggtgcg gatgccgtac gccagaaggt gccagcaatg
     5761 ctgaacagcg atgtgctgcc cgcctcccgc gtttatcccg gctcatcacg tcaggcgtac
     5821 ctggctaacc gtgcgcttca tgagttacga aaaaacggtg cgcttccggt tgatgaagcc
     5881 tgggtcgatg attttatcag ggaagctttc ggacctatgg tggaagagga cgactggaaa
     5941 gacagcacta aggtaaataa aaaggcagaa aaactctgga atatatctct gatcgatcaa
     6001 ctgataacgg aagtcataca gagctcccat tccagagcgg cggcactggc ggttgactct
     6061 gccgccgcaa aactgatgca gaatgcagaa aatgtcagcg aatatctgtc attacgtcat
     6121 caggggctgc agcaaagcat tcagtcacta caggcgcata ttaccagcct gctggcggat
     6181 atccaggaga ttgaggaatg tcagaatcag gtgaccggtg atgtcagaat ggccatggag
     6241 gacatcaata ccaaaaccgg ggaattactg acgaaggtct gtgcctcact ggaggaggag
     6301 ctgaacgact atttcagaag cggtaaacgc aaagaacagc aaatgctgga ggaagaaaat
     6361 tcagcgcagc cccgggagag aaacgcattc gctttctttc acgatatttt cggaaccgga
     6421 aatcagcatg acaggatgcg ggactttgat ccggacagcc ccgaaataaa attcagtgat
     6481 cgcaggg
//
LOCUS       DAZUGK010000228         6426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZUGK010000228 DAZUGK010000000
VERSION     DAZUGK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.230"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..117)
                     /locus_tag="ACHY5W_004122"
     CDS             complement(<1..117)
                     /locus_tag="ACHY5W_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448896.1"
                     /translation="MLQSSADKACNVLVKKVCYLKDVRKITKMMNGKLIYSWL"
     gene            195..1289
                     /gene="ddlA"
                     /locus_tag="ACHY5W_004123"
     CDS             195..1289
                     /gene="ddlA"
                     /locus_tag="ACHY5W_004123"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4448897.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKHLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1313..1525)
                     /gene="yaiZ"
                     /locus_tag="ACHY5W_004124"
     CDS             complement(1313..1525)
                     /gene="yaiZ"
                     /locus_tag="ACHY5W_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFS4448898.1"
                     /translation="MNLPVKIRLDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1785..2093
                     /gene="yaiY"
                     /locus_tag="ACHY5W_004125"
     CDS             1785..2093
                     /gene="yaiY"
                     /locus_tag="ACHY5W_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFS4448899.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2152..3246)
                     /gene="yaiW"
                     /locus_tag="ACHY5W_004126"
     CDS             complement(2152..3246)
                     /gene="yaiW"
                     /locus_tag="ACHY5W_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFS4448900.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSRAPQPLKKGEKEIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3259..4479)
                     /gene="sbmA"
                     /locus_tag="ACHY5W_004127"
     CDS             complement(3259..4479)
                     /gene="sbmA"
                     /locus_tag="ACHY5W_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFS4448901.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4831..5988
                     /gene="ampH"
                     /locus_tag="ACHY5W_004128"
     CDS             4831..5988
                     /gene="ampH"
                     /locus_tag="ACHY5W_004128"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFS4448902.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVTLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5989..>6426)
                     /locus_tag="ACHY5W_004129"
     CDS             complement(5989..>6426)
                     /locus_tag="ACHY5W_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448903.1"
                     /translation="GITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQTITLI
                     EQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMN
                     YIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1615 a         1656 c         1570 g         1585 t
ORIGIN      
        1 aagccaggaa taaattagct tcccgttcat catttttgtg atcttgcgca catctttgag
       61 ataacacact ttcttcacca gtacgttgca cgctttgtca gcagaagatt gcaacaatct
      121 ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg tgttagacgg
      181 gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt aaatcagcgg
      241 aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat aaaagtcgct
      301 tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc gatgccagca
      361 attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc
      421 tcgcccaggt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac ggtcagccgt
      481 tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa gatggttcct
      541 tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt ctggcttcag
      601 cagcctgtat ggataaagat gtcaccaaac atctgctacg cgatgccggg ctgaacattg
      661 cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc gaagtggagt
      721 ctaaactggg gttaccactg tttgtaaaac cggctaatca gggctcttct gttggtgtca
      781 gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga tctggcgttc gagttcgatc
      841 ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat cgaatgcgca gttctgggca
      901 acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat ttctatgcct
      961 acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca gccattgcgc
     1021 cagaaatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg
     1081 caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga
     1141 tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg caagccagcg
     1201 gtctgggtta caccgatctg atcacacgtt tgattgagct ggcgctggag cgtcacgctg
     1261 cggataacgc attgaaaacc acaatgtaat attttaacgc ccgataatat tcttattggg
     1321 cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc cgccagccag aaactaatca
     1381 cccacgtcac cagaccgacg gcataggtct gccaaccttt tgcttcaaat cccagtaacc
     1441 ccaccacgcc attaagaatg aatataaggc caatggcgaa cgcatagtag tgccagtcaa
     1501 ggcggatttt tacaggcaga ttcatggctg ctccgttaac gaaaaacaac atccttgcat
     1561 attctgtaac aaccgtctgt ggtgtagaca acattttcgc aattgttaat aaatttaact
     1621 taaagttgct attttgtgac tcgcagcgaa gattcacgct tcgggaatat tcccaacatc
     1681 aatttaccat gattctgata ttgacaatct aaatgacctg tcagacttgc cataagccgc
     1741 tggtatcaca ccagaagaga agcagatgac ttccggaggt ttttatggct gatttcaccc
     1801 tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct ggcaacattg
     1861 cctatgcgtt gtttgtgctg ttttgctttt gggctggggc gcaattgctg aacctgttag
     1921 tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg
     1981 tggaaattgg tttaggtgtc ggcaccattt tcgggctgat cccgttttta gtaggctgcc
     2041 tcatttttgc agtggtggcg ctatggctgc actggcgaca tcgccgccag taagatttac
     2101 gttgccagat acggcctaaa acgccttatc tggcatgaca ttcctgtttg atcactgttt
     2161 catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag tcaggttgcg
     2221 ggtgattttc gggctttcca gttgaatgcc aggcaacatt tcgcggggga gtgatttacc
     2281 cgttttcgct tcggcaagtt gataaacttt cttgtacagc gccgtctcct caaaagagaa
     2341 gctgtcacct ttctctagct gacgacgaat ttcgctgtcg ctcatcccca gttttgccgc
     2401 cagtttgcgc gttgccagtt cggttttccc gggttcttta ctgtcgtaac gaattaaatc
     2461 accgtccagt gccagcttca cgccgctggc cttactgacc gcgttctgaa acgcggcatt
     2521 acggctggcg taccagccag cgttaaaatc agcaaaacgg tatatcggtg cgctataact
     2581 ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc gactaaagac
     2641 ttcctgacgg actgtaccgt ccattttcca cggataccct ttggtatgct gttcggcaaa
     2701 agcaatgctg acctgcatcg gcccaccggt gcgcaccgga ttaagcgaac caaacagcgt
     2761 ctgccccatt ggcaccatgc tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt
     2821 acgcaccgaa tctaaccgtt cgctataact tttgccgttt ggcgatttga tttttagcgc
     2881 ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca
     2941 ggcgatcttg cttaagcccg gaacggccgg atctgcctga taatttgact cctgctgcgc
     3001 caccgccagc accgagcaga cgttttccag cgtcggtgcc agcccctgac tttcaaaggt
     3061 ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg
     3121 cacgacgctc gccacatcta tctctttttc gccctttttc aacggttgcg gagcccgact
     3181 actacacccc gctaaaacca gaaccgccaa caatgacagc gaagatagag gattgacgcg
     3241 cgacattact tctccttttt agctcaaggt atgggttact tcctgaattt tgtcaccatc
     3301 cagctcatgt tcaaagctgc gcagacgttt gtagatagac atcaactcaa ccagtgtggt
     3361 ccatgagtta atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt tggtaatctg
     3421 cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca agaacaaacc
     3481 gaaaacgtta tcgacctgca aatagagaat gcgggcgatg ttgaaataca tatagtgaaa
     3541 atagaggcgg aaatagtttt tccgtacggc gctaaacagc tcgcgtaccg ttggcggcgt
     3601 cgcgcgcgtg gcatcgtctt caccataaac cagctcttta cggtaggcag cctctactcg
     3661 ctggttttta aactccaacc ccggcagttt gatccctacc actgccagca atccggtccc
     3721 catcagcgac cagacgatag cggcaatcac cagaccatac ggaatgtgcc cgataatcgg
     3781 cagctccggc acatgcgcgg agagcgttac cagcaccggc aggaaggcga tcaacgtcat
     3841 gatggcattg ataaaactga cacccatatt ctccaacgtt gaagcaaaac gcatggtgtc
     3901 ttcctgcaca cgctgtgcgg cgccttcgat atgacgcagt tgttgccagt tcgccatgta
     3961 atattcgttc atcgccgtac gccagcggaa cacgtagtga ctgacaaaga agttattcag
     4021 cacactgatc actaccgcga tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa
     4081 ttgttcgata gtgactttat gcggcgaact tagcgcggtt tgaatcagat catagaacgg
     4141 tgcataccag gcgttgacgg caaccccgac ttccaccaaa aaccaggtga cgaagatgat
     4201 cagtgcagta ccgagtattg accagtattg ccaacggtgc gggctgtaga taaaccagaa
     4261 caatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga aatccaacga
     4321 ccagaaacgg gcggcgctaa tcgggatctg cccggaagct ccggtgatac gcgccaccca
     4381 gtcaccccca ccggcttgcc agaagataac ggcaatcaat gcccaaacaa aggccgagag
     4441 aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata tgcactcctg ctaacttctt
     4501 atcgttttaa caactgctgt gtattaaccg catgaccact acaaagcgtc agccaaatga
     4561 aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc agcctgacca taagaattgt
     4621 ttcgacggat ttcacaaacc aaagattgtt tacataagcg gattaacacg cgcaggatca
     4681 caaatgcagg cattggtgag caggaaaaag atcgtgttta cgcgccgtta cgtttttaac
     4741 actattattg cactaattcg ccctttgcaa tccatcaatg agtagtataa atacgctcag
     4801 ttaccttcat tcaatttatg gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg
     4861 ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc cgatcactga accggagttt
     4921 gcctctgata ttgtcgatcg ttatgccgat catatttttt acggcagcgg tgccacgggg
     4981 atggcgctgg ttgttatcga cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga
     5041 cctggtaata acgttcgccc gcagctggat tcggtcgtgc gtattgcttc cctcaccaag
     5101 ctaatgacca gtgagatgct ggtgaaattg ctcgaccagg ggacagtgac gttaaacgat
     5161 ccgctaagta aatatgctcc gccaggcgca cgcgtgccaa cctacaatgg gacgcccatt
     5221 acgctggtca atctggcaac ccataccagc gccctgcccc gtgaacagcc cggtggcgcg
     5281 gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac gctggaaata cctttctacg
     5341 gcgaagctga aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc gttcgacctg
     5401 ctggcagatg cactggcgaa tgcgtccggc aagccttata cccagttgtt tgaagagcag
     5461 attacccgtc cattggggat gaaagacacc acctacaccc cctcaccgga tcagtgccgc
     5521 cgtttaatgg ttgcagagcg cggtgccagt ccatgcaata acacgctggc ggcaattggc
     5581 agcggcggcg tctattccac gcctggcgat atgatgcgct ggatgcagca gtatttgtca
     5641 tctgattttt atcagcgcag taaccaggca gatcgcatgc agacgttgat ttatcagcgc
     5701 gcgcagttta cgaaagtgat tggcatggat gtccccggca aagcggatgc gcttggttta
     5761 ggctgggtat atatggcccc gaaagagggt cgtccgggga ttattcagaa gacaggtggt
     5821 ggtggaggtt tcattaccta tatggcgatg atcccgcaga agaatattgg cgcgtttgtg
     5881 gtggtcactc gttcgccatt gacgcgcttt aaaaatatga gcgatggcat caatgacctg
     5941 gtgaccgagc taagcgggaa taaaccgttg gttatccccg catcctgatt aatactctga
     6001 aggcaaacgg ttgatagcga ccagtttgcc tttattcatt tcgatatagc cgcctttacg
     6061 caaagcagcg agaacttctg cgacgacaga acgcgatatg cgtgtacgtt gatggatata
     6121 attcatcaca ccaatacgtg atcgcaattc ttcattccag tcaatcattg ataataatgt
     6181 tgcgcggatt tgttcgtagg aattatgccc aatgagctgc acgtcgcgta attccagaat
     6241 tctattttgc caggctaacc agtaaaaagc gtctcgccag agttgatttt gttcaataag
     6301 cgtaatggtt tgtttggctg gtagatgata tcccgtacaa tttccttctg atattaattt
     6361 gtatggtata tcgtttttca ttaaaccatc agccagcccc ataatataag gagcctgggt
     6421 aatacc
//
LOCUS       DAZUGK010000229         6416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZUGK010000229 DAZUGK010000000
VERSION     DAZUGK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.231"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            70..1125
                     /gene="phoE"
                     /locus_tag="ACHY5W_004130"
     CDS             70..1125
                     /gene="phoE"
                     /locus_tag="ACHY5W_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS4448904.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDIYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     MQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLNSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHY5W_004131"
     CDS             complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHY5W_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS4448905.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHY5W_004132"
     CDS             complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHY5W_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS4448906.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDNYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSMLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKALQEITNWIEKRLC"
     gene            complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHY5W_004133"
     CDS             complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHY5W_004133"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4448907.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3678..5135
                     /gene="pepD"
                     /locus_tag="ACHY5W_004134"
     CDS             3678..5135
                     /gene="pepD"
                     /locus_tag="ACHY5W_004134"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS4448908.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVAHYWTLLTELLKEIPAK"
     gene            complement(5192..5806)
                     /gene="prfH"
                     /locus_tag="ACHY5W_004135"
     CDS             complement(5192..5806)
                     /gene="prfH"
                     /locus_tag="ACHY5W_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFS4448909.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVVVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVRKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5803..>6416)
                     /locus_tag="ACHY5W_004136"
     CDS             complement(5803..>6416)
                     /locus_tag="ACHY5W_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFS4448910.1"
                     /translation="SILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINSQMIA
                     LRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSW
                     LVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIF
                     EEAPQAYKSAESVVQCLVQAGLIIPVVRLRPVLTLKNSGGKKG"
BASE COUNT         1632 a         1649 c         1567 g         1568 t
ORIGIN      
        1 aaaactttcc tgttttcaac ggggctctcc cgctgaatat ccgcgcgtta attaaaatca
       61 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgca
      121 tccgtacagg ccgcagaaat atataacaaa gacggtaata aactggatat ctatggcaaa
      181 gttaaagcca tgcattatat gagtgataat gacagtaaag atggcgacca gagttatatc
      241 cgttttggtt ttaaaggcga aacacaaatt aacgatcaac tgactggtta tggtcgttgg
      301 gaagcggagt ttgccgggaa taaagcggag agtgatactg cacagcaaaa aacgcgtctc
      361 gctttcgccg gattgaaata taaagatttg ggttctttcg actatggtcg taacctgggc
      421 gcgctgtatg acgtggaggc ctggaccgat atgttcccgg aatttggtgg cgactcctcg
      481 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
      541 gacttcttcg gcgttatcga tggtctgaac ttaaccctgc aatatcaagg gaaaaacgaa
      601 aaccgcgatg tgaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
      661 ggcggcagcg atttcgctat cagtggtgcc tataccaact ctgatcgcac caacgagcag
      721 aacatgcaaa gccgtggcac aggtaagcgt gcagaagcgt gggcaacagg tctgaaatac
      781 gatgccaata atatttatct ggcaactttt tattctgaaa cacgtaaaat gacgccaata
      841 tctggtggtt ttgctaataa gacacagaac tttgaagcgg ttgctcaata ccagtttgac
      901 tttggtctgc gtccgtcgct gggttatgtc ttatcgaaag ggaaagatat tgagggcatc
      961 ggtgatgaag atctggtcaa ttatattgat gtcggtgcca cgtattattt caacaaaaat
     1021 atgtcagcat ttgttgatta taaaataaac caactgaata gcgataataa gctgaatatc
     1081 aataatgatg atattgtcgc ggttggcatg acctatcaat tttaatgaat attgccggat
     1141 gtgatgcatc cggcacattt cattcacgcc gttaacttca ccggttcatc acgaaaatca
     1201 tccgccggtt ccagcttcag attcagcgtc gtcagcagct cacgcagctt ctcgtggaac
     1261 tcacgcaggg tgtgctccag tcgttcaacc acttcagtgt cttttaccgg aacactcttc
     1321 cagtcgcctg ctttatcgaa cagaccaaac tggtaactgt atgtaaaacg ggattcctgt
     1381 gcttcaagct ccatccacca gccccagaac tcacgcactt ccggtgccgg tttcacgttg
     1441 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     1501 tatgggccta gtgcggtaaa ttttttgatc aatctgcttt tcgggtgtcc actcggtaac
     1561 gtcattgcga tctcctgttg tgatgcaact gttttaccaa attgacaaaa tttagcaaat
     1621 ttttaacaca atcgcttttc gatccagtta gtgatttcct gaagcgcctt gtcaaaattc
     1681 cgatacacgg ggttaaacgg aatctctaat aatttaccat cagcagatga cgacgtgatt
     1741 aaacgtgaat cctcttccgg gctaaacgga tcgttcttcc agtagcctga taacattggc
     1801 gttgggcaac gacgccccag caatccttgc acttttaatg agtagcgatt cagctcaaca
     1861 cgcaacgctt cgtcggaagc atcatgcatc cccaaacgac tggccagaac gtcaagatac
     1921 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     1981 agacaggcaa ccgctttcag acgcggcgat tcaaggtatg ccagacgcac ggcaacgtta
     2041 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     2101 agcgctttta atacatgctg gtgcagcatg ctggaatcct gcgttaactt ccattttgac
     2161 gaaaacccca ccgagggcat atcaatggtc aacattgcaa tgccacgtgg cgcaaaatat
     2221 cgctcataca ggctgtagtt atccgtttgc attgcatcca ggccaccgca cataagaacc
     2281 gttggaaacg ggccatcacc ttttggcata tgcaaaaagc cggtgatggg cgcaccgccg
     2341 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgagcggc ctcttcatag
     2401 gcgcggtttg acaaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     2461 gcggcaatgt tgtacaacgt agccgcatgc agccagtgac gaccactgag ttgcggatct
     2521 tcttccgcac aggctttttg ttgccacacc atcgcctggg tggcccactc ataaatccag
     2581 ttgccgccgc ggtagccaat taccgtatcg tataaatcat cgtcggtacg ttcggcatcg
     2641 ctcatcacaa tacgcgcctg gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc
     2701 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac
     2761 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
     2821 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     2881 agaatactta ctggaaacta ttgtaacctg cctgaagtta aaaagaacaa cgcccggcaa
     2941 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
     3001 ccacggctgt tcgatccagg tatcttgcgg gatatcaaca acataatcat caaccagcgg
     3061 acgaccagcc ggttttgcaa agatggtgac aaagtgcgct tttggataca tttcacgaat
     3121 cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
     3181 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     3241 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     3301 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     3361 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     3421 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     3481 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     3541 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     3601 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     3661 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     3721 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     3781 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     3841 tcctgattcg taaacctgct acagcaggta tggaaaatcg taaaccggtc gtcttacagg
     3901 cccacctcga tatggtgccg cagaaaaata acgacaccgt gcatgacttc acgaaagatc
     3961 ctatccagcc ttatattgat ggcgaatggg ttaaagcgcg cggcaccaca ctgggtgcgg
     4021 ataacggcat tggtatggcc tctgcgctgg cggttctggc tgacgaaaac gtggttcacg
     4081 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct
     4141 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     4201 aaatctacat gggttgtgcg gggggtatcg acttcacctc caacctgcat ttagatcgtg
     4261 aagcggttcc agctggtttt gaaaccttca agttaacctt aaaaggtctg aaaggcggtc
     4321 actccggcgg tgaaatccac gttggcctgg gtaatgccaa caaactgctg gtgcgcttcc
     4381 tggcgggtca tgcagaagaa ctggatctgc gtcttattga tttcaacggc ggcacactgc
     4441 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     4501 ccctgaaatc tctggtgaat acctatcagg agatcctgaa aaacgagctg gcagagaaag
     4561 agaaaaatct ggccttgttg ctggactctg tagcgaacga taaagctgcc ctgattgcga
     4621 aatctcgcga tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaatt
     4681 ccgacgtggc aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg
     4741 acaataacgt agaaattcac tgcctgatcc gttctctgat cgacagcggt aaagactacg
     4801 tggtgagcat gctggattcg ctgggtaaac tggctggcgc gaaaaccgaa gcaaaaggcg
     4861 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     4921 atcagcgcct gttcaacaag acgccgaaca tccagattat ccacgcgggc ctggaatgtg
     4981 gtctgttcaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca acaatcaccg
     5041 gtccacactc tccggatgag caagttcaca tcgaaagcgt agctcattac tggacactgc
     5101 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     5161 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtga
     5221 acgttcgtcg cgggttgcca cgttcaatct ggtgatggaa cattcgccgc tgcgatttcc
     5281 gcactgcgct attttcctgt tgctgttgct ccagcttcca ggcaatcagc aatcgtgcca
     5341 gccgcttgtt agcatgctga ctgcgttctg actgaacctt cacgctaata ccggatgcca
     5401 aatgcgtggc gcgtaccgcc gagtcggttt tattgacatg ttgaccaccc ggccccgacg
     5461 aacgcagcgt ctcataccgg atcgcatcgg attgctcctg ctcatcagcg gtaaaacgcc
     5521 caatgcccag aaaccagttt ttgcgcccat gatgaggccg atacggactc ggacaaatcc
     5581 actgaatagt gccgcaccaa ctttcgctta acgcccatgc gttgtcgcca tccagagaaa
     5641 tcagcgccga acgcagcgtg tcagagtaac gacctgtttc tgtttccagc actgttacca
     5701 cgacgtcttg tcgagcagct tctttaatca gcctgtccag cgcctttttc actgcgagac
     5761 aacattcttc cggcccctga gcagaggaga gttgtagcaa gatcatcctt ttttccctcc
     5821 actgtttttg agcgttagca ccggacgcag ccgcacgaca ggaataatta atccggcctg
     5881 caccagacat tgcaccacgc tttcagccga tttataggct tgtggcgctt cttcaaagat
     5941 gagttgttta tcgcgacaaa ttacccgact gccaagttca gtacgcgaga gttgcgtcgc
     6001 cgtgtatttc gctgccagac gccctttaca ctcggtgcgc ccccatttac gccctgcccc
     6061 atgcgccagc gagtggagcg ttttttcgtt cgcaacaggc tgtacaagcc aggagtaatc
     6121 accgcgtgaa ccgggaataa tcaccagacc gttgtcatcc ggcgtggcac ctttacgatg
     6181 caaccagccc tgttgatcgc cgatttgaca cgcgctaaca aagttatgcg ccacatccag
     6241 aaccggactt cccgtggcct taacctgttg cattatgcgc aaagcgatca tctggctatt
     6301 aatacgcgca aatgccagcg catcgtcatg ttccgcaatg taccgtagcg catcgtcact
     6361 gccttcaggc aaaccatgat gcgaaaacga ggcaatatgc cgctgtaaaa tagact
//
LOCUS       DAZUGK010000230         6392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZUGK010000230 DAZUGK010000000
VERSION     DAZUGK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.232"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            96..1004
                     /locus_tag="ACHY5W_004137"
     CDS             96..1004
                     /locus_tag="ACHY5W_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS4448911.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKILAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1224..3215)
                     /gene="tkt"
                     /locus_tag="ACHY5W_004138"
     CDS             complement(1224..3215)
                     /gene="tkt"
                     /locus_tag="ACHY5W_004138"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4448912.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3493..4251
                     /gene="loiP"
                     /locus_tag="ACHY5W_004139"
                     /gene_synonym="yggG"
     CDS             3493..4251
                     /gene="loiP"
                     /locus_tag="ACHY5W_004139"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFS4448913.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4570..5490)
                     /gene="speB"
                     /locus_tag="ACHY5W_004140"
     CDS             complement(4570..5490)
                     /gene="speB"
                     /locus_tag="ACHY5W_004140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFS4448914.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5626..6357)
                     /locus_tag="ACHY5W_004141"
                     /pseudo
     CDS             complement(5626..6357)
                     /locus_tag="ACHY5W_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1542 a         1706 c         1676 g         1468 t
ORIGIN      
        1 ctatacccct ttatagattc tgaattctca cctgtcggct gttttctcct catctcatgt
       61 gacatattcc cacactttac tcaaggaaga ttgtcatgaa agcacctgtt tcattaacct
      121 catttcgccc acaaaaatct cttgctatcg ccattggcgt gcttgccgtg gttgtgctgc
      181 catttctctc gtattacacc gttaatgaag gtgaacgcgg cattttgctg cgctacggaa
      241 aaatcgtaaa agttgccgag ccgggtctgg gttttaagat cccgtttatg gaaagcgtgg
      301 agaaaatttc cacgcgtaat caggctgtgg tctatcaggg attacaggcc tacagccgcg
      361 accagcaacc ggcacaaatg acagtatcag tcagcttcca cattaaacct tcagaagcag
      421 gcgcggttta taccacctac aacaccattg aagcattaaa agatcgtctg attgtccgtc
      481 agttgccaac tcaactggag aacgttttcg gccaatatac cgccatcagc gctgtacagg
      541 atcgtacaaa actggttcag gatctgcaaa atgccatgcg caaagccgtt gttgggccag
      601 tggtaattga cggggtccag attgaaaata ttgatttttc tgacgcttat gaaaagtcga
      661 ttgaagaccg aatgaaagca gaagtcgcca ttgccacgcg taagcaaaat ctggaaacag
      721 agaaaatcct ggcgcaaata gcggtaactc aggcccaggc agaagcagac agtaaactgg
      781 cggcagccaa agcagaagcg gaaactattc gcgtaagagg cgcggcagag gctgaaacta
      841 ttcgtttaaa aagtgcggct gaagcagaag caatccgcct gcgcggtgaa gctctgcggg
      901 ataatcccgg acttgtagcc ctcaccactg ccgaacgttg ggacggtaaa ctgcccgata
      961 ccatgatccc cggcagcaca gttccgttta tctctactaa gtaaaaaaag ggccgctttc
     1021 gcggcccttg agagtgatgg caaaagcaaa actgcctgat gcgctacgct tatcaggcct
     1081 acatacttcc tgcaatatat tgaatttgca cgatcttgta ggtcggataa ggcgttcacg
     1141 ccgcatccga catgaacaac gcgcacttta ccaataatct aaagggccgc tttcgcggcc
     1201 cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac aacgttgtca
     1261 acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc gaaggtggtc
     1321 ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc aatacccgct
     1381 tccaccgcaa cgcgtgcagt aacagctttc ggcaggacag actcacggta agccgcgtcc
     1441 tgcttgtcga atgcgtcggt agacggcatg gaaaccacgc gcgctttcac gccttcggca
     1501 gtcagttttt cgtatgcagc gacagccagt tcaacttcag aaccagtagc gatgaagatc
     1561 agttccggct ggccggcaca gtctttcagc acataaccac cgcgcgcgat gtttgccagt
     1621 tgctcttcag tacgctcctg ctgcgccagg ttctgacggg agaggatcag cgcggtcgga
     1681 ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg gtcacacgga
     1741 cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac cggctggtga
     1801 gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt
     1861 ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg
     1921 gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc ggtcataccg
     1981 aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa
     2041 ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag gaattccggc
     2101 aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc gattttcgcc
     2161 ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc agacggcatt
     2221 tcgcctttca tacggcgggt aaattcagct gcttcctgcg gataagcttt cgcgtaagcc
     2281 gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc atcccactga
     2341 gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg ttcgcgggtc
     2401 agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc
     2461 ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg
     2521 cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat gtcgcgaata
     2581 acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg
     2641 tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc
     2701 gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg
     2761 aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg cgccgccagc
     2821 gttttttctg caatcgccat acctactgcg ttggcaatac cctgccccag cggaccggtg
     2881 gtggtttcca caccagcggt gtaacccact tccgggtggc ccggagtttt agagtgcagc
     2941 tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg
     3001 tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac
     3061 ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata
     3121 cccatagggg caccagggtg accggatttg gctttctgta ctgcgtccat gctcagcgca
     3181 cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag
     3241 ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac
     3301 aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgctgctt cagaaatctc
     3361 tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact
     3421 gagactgagc gtcggttcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc
     3481 ggaagaaaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg
     3541 accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga ggcttttcag
     3601 gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg tcaggagatg
     3661 gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt
     3721 gccaatgcgc taggcaacaa tatcaacggt cagccggtaa attacaaagt gtatatggcg
     3781 aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag cgggctgatg
     3841 gatatgatga cggataacga agtcgaagcg gtgatcggtc acgaaatggg gcacgtggcg
     3901 ttaggccatg tgaagaaagg gatgcaggtg gcacttggta caaatgccgt gcgagtagct
     3961 gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaactcgg tgatctgggc
     4021 gagaaattag ttaattcgca attctcccag cgccaggaag cagaagccga tgattattct
     4081 tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa
     4141 ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc ggcatccgcc
     4201 gaacgcgccc agcatattcg cgatcgtatg agcgcggacg ggattaagta aggcatggtg
     4261 gtgccaaacg tagttcagat aaggcattta cgccgcatct gacataaatg gcacattcac
     4321 tacctcgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta cgggtgccaa
     4381 acgtaggtca gataaggcgt tcacgccgca tctgacatga atggcacatt tgttaccttg
     4441 tgcgcattgc cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg
     4501 tcggataagg cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc
     4561 atccggcgct tactcgccct ttttcgccgc ctgaatatac agcatttcca gcgccagcgt
     4621 tgccgctgcc agagcagtga tttccgactg atcgtatgcc ggagccactt ccactacgtc
     4681 catcccaacg atgttgagat ctttcaggcc gcgtaccagt ttaatagcgc gatcggaggt
     4741 caggccgcca attaccgggg taccggtgcc tggtgcaaaa gcaggatcca ggcagtcgat
     4801 atcaaaggtc aggtaaaccg gcatatcacc cacaatttgt ttcacctggg cgataacgtc
     4861 atccacgctg cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc
     4921 gaactcagta cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg
     4981 cgcggtatag aacatggtgc cgtggtcaaa ttcacaaccg ttcgcgtagg tatcggtgtg
     5041 ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg
     5101 cagcgtaaca aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt
     5161 ctcggcgtgt gcctgcagtt tttcgctcat ctcacgggca tcgccgaagg catataccag
     5221 atcgccgcag tccaccacgt tcagacgctc gcgcatgtcg aaattccacg ggaagcggtt
     5281 gtgttcccag gccaggttag tcgaaacctg gcgaatcgcc gccgggccgt gacgaccacc
     5341 ggcacggcca gaggtggcca tgtcgaacgg cacgccggtg atcacccagt cggcatcgct
     5401 gtcatacggc tggaagttca tcggcaggcg taaaaaacca aaggcgttgg aaaccagtga
     5461 attatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa
     5521 aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt
     5581 tgtgctaatt atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg
     5641 tggcggcgca tagccattgt tatctgccgg aaaggcttgt ccagagcacg gtggcagcgc
     5701 gttttacgcc tggccttgta attcaaaggc ggcaaagcct attacaccgg tattacgcac
     5761 atcgccttgc gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc
     5821 ttcggtatgt ttatctttgc ggaatccttt aatcgctaac gaatctccgg cattcacaat
     5881 atagccgttg ttattcagcg aaccttgctt gccgttcagc acatccaggc catcaaccgt
     5941 agcaacgatc tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat
     6001 tccgactctg gcatggacaa tatagcgtgg ttcattattg gatgaatcat aaacacgcgt
     6061 aataggtaga gaattaaaat taccatcgcg aatagcatat tccagttcgt taatacgaat
     6121 gctataaacg cggtcgtagc cggaaggaac attagtcgaa taactaataa cgatcatctc
     6181 cgccggatcg cgccaggctc gctcagcaga tacgccctga accgttgagt gcacatcccc
     6241 accccaggcg gtgccgacat tgcccgatgc actgctatta tactgcgcat tactggaaca
     6301 accggccagc agcacgatta atgccaccaa cgcgctacgt actttccatt ttttcataac
     6361 cgttccctct gcatttaatt aatgcagcga tt
//
LOCUS       DAZUGK010000231         6290 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZUGK010000231 DAZUGK010000000
VERSION     DAZUGK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.233"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(330..2000)
                     /gene="betA"
                     /locus_tag="ACHY5W_004142"
     CDS             complement(330..2000)
                     /gene="betA"
                     /locus_tag="ACHY5W_004142"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFS4448915.1"
                     /translation="MQFDYIIIGGGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAKWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2014..3486)
                     /gene="betB"
                     /locus_tag="ACHY5W_004143"
     CDS             complement(2014..3486)
                     /gene="betB"
                     /locus_tag="ACHY5W_004143"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFS4448916.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHKLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3500..4087)
                     /gene="betI"
                     /locus_tag="ACHY5W_004144"
     CDS             complement(3500..4087)
                     /gene="betI"
                     /locus_tag="ACHY5W_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFS4448917.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4216..6249
                     /gene="betT"
                     /locus_tag="ACHY5W_004145"
     CDS             4216..6249
                     /gene="betT"
                     /locus_tag="ACHY5W_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFS4448918.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     LMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1258 a         1744 c         1755 g         1533 t
ORIGIN      
        1 gctccacgtt gttttggtct gacttatttt tgtcctcgtt acaagaagcc ttttcgtata
       61 tgctaaccgt gagcgggagt gaacaagtca ccatagccag gaacactagc ggcgtaattc
      121 ttaagctgag tggcatttca cctgcggttt atcagtgcat tcatagtacc ttcatcagcg
      181 agattgaacg agtcagctta gaatttgctg gcgaggttgt cactatgaag catgagagtt
      241 acccctatgt tttatataac gattctgtcc ccatacctgg aacggtaact ctcaatctga
      301 tcggttcctg cgttagttca catcacgact cattttttcg ctctcaccgg catcccattt
      361 gccacaaaat atcccgccgt gctcctcggc agcgcttcct gtccacgaat catgtccgct
      421 attttctcgc caatcataat tgtcgtggcg ttcaaattcc cggtgataat ctgcggcata
      481 attgacgcat ccaccacgcg caggccttcc agcccatgca cgcggccttc gccgtcaacc
      541 acggacatct cgtcgtagcc cattttgcag gtaccgcacg gatggaaggc ggtttcggcg
      601 tgattacgca cgaactcatc gagctgctca tccgtctgac attccgtgcc ggggctgatt
      661 tcgcggccac gatactgatc cagcgcgggt tgatgcatga tctcgcgggt gatgcgaatt
      721 gcgtcgcgga attcctgcca gtcctgttcg tgcgacatgt agttaaacag aattgccgga
      781 tgctggtgcg ggtcgcggga tttaatccgc acatgcccac ggcttggcga gcgcatcgat
      841 ccgacgtggc actggaagcc gtgctctttc actgcattcg agccgttata gttaatcgct
      901 actggcagga aatggtactg aatattcggc cacgcaaatt cctctcggct gcgaataaat
      961 ccgcctgctt caaagtggtt gctggcacca acgccggtgc cgccaaacag ccacttcgca
     1021 ccgattttcg gctgattcca ccactgcagg gcagggtaga gggaaaccgg ttctttgcac
     1081 tcatattgca gatacatctc cagatgatcc tgaagatttt cgccgacgcc gggtaattca
     1141 tgcaccagcg gaatatcaaa ctccgccagc agttcagcgc tacccacgcc ggaacgttgc
     1201 aggatctgcg gagaggcgat cgcacctgca cataacagca cctctttatt cgctgtcgcg
     1261 cgggttggaa tggtgctatc gccttccagc cactcgacgc ctaccgcgcg tttgccgtca
     1321 aaaatgatgt gatcggtcat agcgtgagtt cgaatggtca ggttcgggcg cgatttggcc
     1381 tgatcgagat aaccgcgcgc ggtgctggcg cggcggccct gcggcgtgac ggtgcgatcc
     1441 atcgggccga aaccttcctg ctgataaccg ttgagatcgt ccgtgcgcgg gtagcccgcc
     1501 tgcacgcccg cttcaatcat cgcttcaaac agcggattga cgccgggttt ggaggtggtg
     1561 acgctcaccg ggccgtcgcc gccgtggtag tcgttctcgc ccatatcgcg agtctcggcc
     1621 ttgcggtagt agggcaggca gtcgaggtag ctccagttct ccagaccggg ttcttgcgcc
     1681 cagttatcga gatccagcgc attgccacgg atgtagcaca tgccgttgat cagcgacgat
     1741 ccacccagac ctttaccgcg tccgcactcc atgcggcggt tattcataaa cggttcaggt
     1801 tccgtctcgt aagcccagtt gtagcgttta ccctgtagcg ggaatgccag ggcagcgggc
     1861 atctgggtgc ggaagtcaaa gcgatagtcc gggccgcccg cttcaagcag cagcacggag
     1921 gtattcggat cttcagtcag acgggtagcg agaacgttgc cggctgagcc gccaccaata
     1981 atgatgtagt caaattgcaa ataaacctcc tggttagaat atggactgga atttagccat
     2041 ctcaacctgg atggacttca cctgggtgta actctggagc gtcatcacgc cgttctcgcg
     2101 accaatgccg gagtgtttgt agccgccaac gggcatctct accggggatt cgccccaggt
     2161 gttgatccag caaatacccg cttccagctt atgaatgacg cgatgcgcgc ggttcaggtc
     2221 cgccgtcacg attcccgccg ccaggccgta gtcggtatcg ttagcgcggc gaatgacttc
     2281 gtcttccgac tcgtaggtca gaatggacat cactggcccg aagatctctt cacgcacgat
     2341 ggtcatatcg tcgctgcaat cggtgaacac agtcggcgcg acccatgcgc cgttatcgag
     2401 gccatcgcct ttcagtacat cgccgccgca cagtacgcgc gcgccttcct ctttgccttt
     2461 ggcgatatag cgcagcacgt tatcgcgatg cgggaagctg accagcgggc cgaagttagt
     2521 ttgcggatcg aaaacgtcgc ccgcgcgaat gcgctcaacg cgcgccagaa ttttctgctc
     2581 aaatgcggct ttgcatttcg ccggaacgaa gacgcgggtg ccattggtac acacctgacc
     2641 ggagctgaag aagtttgcca tcatggcgat atcggcggcg agatcgagat ccgcatcatc
     2701 gaaaacgatc agcggtgatt taccgcccag ttccatggtc acttctttca gggaagaggc
     2761 cgccgagtta gccatcactt ttttgccgct ggcgacaccg ccggtaaatg acactttggc
     2821 aatgcccgga tgctcggtca gatattgccc ggtctccgcg cccacgcccg gcaacacgtt
     2881 aaatacgccg tccggtaggc ccgcttcgct gtaaatttca gccagcttta acgcggtaag
     2941 cggggtgact tcgctcggtt tgaaaatcat tgcgttgcct gccgccagcg ccggggcgga
     3001 tttccacagg gcaatctgaa tcgggtagtt ccatgcgcca atccccgcca ccacacccag
     3061 cggttcgcgg cgggtataaa caaatgacgt ctcacgcaac gggatctggc tgccttccag
     3121 cgccgggatc agcccggcgt agtactccag cacgtccgca ccggtaacga tatcgacggt
     3181 tgaggtttcc gaatatgctt ttccggtatc gagggtttcc agttttgcga gttcgtcatt
     3241 gcgttcacgc agaatatcaa cggcccgacg cagaatacgc gagcgctcca tagcggtcat
     3301 cgccgcccag attttttgcc cctgttgggc actcttcacg gcgcgatcga catcctcacg
     3361 cccggcggcc tgcacggtcg ccagcacgtt accgttggcc gggttaatgg tctcgaaagt
     3421 gcgaccgctg gtggcggagg tataaccacc atgtatataa agctgctgtt ctgccattcg
     3481 ggacatcacg tctcctcggt taatcggtgg gtaaatgctg agtaataaag tgacgggtca
     3541 gcgagtgtgc gagaggttta tccagcggtt tgccgctcag tgccgcgcgc agccataatc
     3601 cgtcaatcag cgcggcaagg ccgtagcccg cttcctgtgc ctggtggcgc ggcaattcgc
     3661 ggcgaaactc gctcaccaga ttcgacagca agcggcggct gctgacctgc tgtaaacgat
     3721 agagcatcgg ctgatgcata ctgctggccc agaacgccag ccaggctttc atcgccgcac
     3781 tgctcacctg cgtttcatcg aagtttccgc caacaatcgc ctgtaatcgc tgctctgcac
     3841 tgccctgcgg aagtgcatgt aatcgattca aaaccgcgtc acgcagctga ctggtgatat
     3901 cgcgcatggt tgcttccagc agaccatttt tgtccctgaa atagtggctg atgatccccg
     3961 tagaaacgcc tgcacggcgg gcgatctgcg cgatcgttgc atcgtgcatg cccacttcat
     4021 ttattgcttc cagtgtggcg tcgatcagtt gtctgcgccg gatcgactgc atccccaatt
     4081 tgggcatttt cgccactcca ttcatcagcg ttgtttatct attaaagcgg ttattgattg
     4141 gacgttcaat ataaaatgtg tcttaattgt tactaatttg attttaaatg gtaacaataa
     4201 cagtggggat actggatgac agacctttca cacagcaggg aaaaggacaa aatcaatccg
     4261 gtggtgtttt acacctccgc cggactgatt ttgttgtttt ccctgacaac gatcctgttt
     4321 cgcgacttct cggccctgtg gattggccgc acgttggact gggtttctaa aaccttcggt
     4381 tggtactatc tgctggcggc aacgctctat attgtctttg tggtctgtat cgcttgttcg
     4441 cgttttggtt cggtgaagct cgggccagaa caatccaaac cggaattcag cctgctgagt
     4501 tgggcggcga tgctgtttgc tgccgggatc ggtatcgatc tgatgttctt ttccgtagcc
     4561 gaaccggtaa cgcagtatat gcagccgccg gaaggcgcgg gacagacgat tgaggccgcg
     4621 cgtcaggcga tggtctggac gctgtttcac tacggcttaa ccggctggtc gatgtatgcg
     4681 ctgatgggca tggcgctcgg atactttagc tatcgttata atttgccgct caccatccgc
     4741 tcggcgctgt acccgatctt cggtaaacgg attaacgggc cgataggtca ctcagtggat
     4801 attgcagcgg tgatcggcac tatcttcggt attgccacta cgctcggtat cggtgtggtg
     4861 cagcttaact atggcttgag cgtactgttt gatattcccg attcgatggc ggcaaaagcg
     4921 gcactgatcg ccttgtcggt gataatcgcc actatctcgg tgacatccgg tgtcgataag
     4981 ggcattcgcg tgttatcgga gcttaatgtc gcgctggcgc tgggattgat cctgttcgtg
     5041 ttgttaatgg gcgacacttc gttcctgctt aatgcactgg tgctgaatgt tggcgactat
     5101 gtgaatcgct ttatgggcat gacgctcaac agttttgcct tcgaccgccc ggttgagtgg
     5161 atgaataact ggacgctctt cttctgggca tggtgggtgg catggtcgcc gtttgtcggc
     5221 ttgttcctgg cgcgtatctc gcgtgggcgt accattcgcc agttcgtgct gggcacgttg
     5281 attattccgt ttaccttcac gctgttatgg ctctcggtgt tcggcaatag cgcgctgtat
     5341 gaaatcatcc acggcggcgc ggcatttgcc gaggaagcga tggtccatcc ggagcgcggc
     5401 ttctacagcc tgctggcgca gtatccggcg tttaccttta gcgcctccgt cgccaccatt
     5461 actggcctgc tgttttatgt gacctcggcg gactccgggg cgctggtgct ggggaatttc
     5521 acctcgcagc ttaaagatat caacagcgac gcccccggct ggctgcgcgt cttctggtcg
     5581 gtggcgattg gcctgctgac gctcggcatg ctgatgacca acgggatatc cgcgctgcaa
     5641 aacaccacgg tgattatggg gctgccgttc agctttgtga tcttcttcgt gatggcgggg
     5701 ttgtataaat ctctgaaggt agaagattac cgccgtgaaa gtgccaaccg cgataccgca
     5761 ccgcgaccgc tggggcttca ggatcgcctg agctggaaaa aacgtctctc gcgcctgatg
     5821 aattatccgg gcacgcgtta cactaaacag atgatggaga cggtctgtta cccggcaatg
     5881 gaagaagtgg cgcaggagtt gcggttgcgc ggcgcgtacg tggagctaaa aagcctgccg
     5941 ccggaagagg gacaacagtt ggggcatctg gatttgttgg tgcatatggg cgaagagcaa
     6001 aactttgtct atcagatttg gccgcagcaa tattcggtgc cgggctttac ctaccgcgca
     6061 cgtagcggta aatcgactta ctaccggctg gaaactttcc tgttagaagg cagccagggc
     6121 aacgacctga tggactacag caaagagcag gtgatcaccg atattcttga ccagtacgag
     6181 cggcacctta actttattca tctccatcgt gaagcgccgg gccatagcgt gatgttcccg
     6241 gacgcgtgat tgttattgca taaaaccgcg ccatgtttgc atatggcgcg
//
LOCUS       DAZUGK010000232         6239 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZUGK010000232 DAZUGK010000000
VERSION     DAZUGK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.234"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..979
                     /gene="pfkB"
                     /locus_tag="ACHY5W_004146"
     CDS             50..979
                     /gene="pfkB"
                     /locus_tag="ACHY5W_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFS4448919.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHY5W_004147"
     CDS             1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHY5W_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS4448920.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1476..2336
                     /gene="yniA"
                     /locus_tag="ACHY5W_004148"
     CDS             1476..2336
                     /gene="yniA"
                     /locus_tag="ACHY5W_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4448921.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2377..2913)
                     /gene="yniB"
                     /locus_tag="ACHY5W_004149"
     CDS             complement(2377..2913)
                     /gene="yniB"
                     /locus_tag="ACHY5W_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFS4448922.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHY5W_004150"
     CDS             3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHY5W_004150"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFS4448923.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3891..4481
                     /gene="ydjM"
                     /locus_tag="ACHY5W_004151"
     CDS             3891..4481
                     /gene="ydjM"
                     /locus_tag="ACHY5W_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4448924.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4614..6005
                     /gene="tcyP"
                     /locus_tag="ACHY5W_004152"
     CDS             4614..6005
                     /gene="tcyP"
                     /locus_tag="ACHY5W_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFS4448925.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(6009..>6239)
                     /gene="ydjO"
                     /locus_tag="ACHY5W_004153"
     CDS             complement(6009..>6239)
                     /gene="ydjO"
                     /locus_tag="ACHY5W_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFS4448926.1"
                     /translation="ILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFA
                     DDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1524 a         1545 c         1573 g         1597 t
ORIGIN      
        1 ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat
       61 ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc
      121 cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt
      181 cgcccgcgcc attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc
      241 gaccggcgaa cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga
      301 agccaaagac tggacccggc agaatttaca cgtacatgtg gaagcaagcg gtgagcagta
      361 tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca
      421 agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt
      481 gaagctggaa aaattaaccc aactgatttc cgctgcgcaa aaccaaggga tccgctgcat
      541 catcgacagt tctggcgaag cgttaagtgc agcactggcg attggtaaca tcgagttggt
      601 taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccagacga
      661 tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc
      721 cctgggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc
      781 accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact
      841 gaaactggca gaaaatgcct ctcttgaaga gatggttcgt tttggcgtag ctgcggggag
      901 tgcagccaca ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta
      961 cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca tcaccaacct
     1021 gtcggcaacg cgtttctccg actatgctca aaagtcatgt gataacaaag gggtgaacta
     1081 tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga
     1141 ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg
     1201 atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa
     1261 gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc
     1321 ctgaaatcac catcacgacc tgggaggaat ggaatagcaa cagaaaataa atggtttttg
     1381 ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa
     1441 ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt
     1501 tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag
     1561 tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa
     1621 gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta
     1681 aaaccgtcac cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg
     1741 tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc
     1801 aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat ttcgataacg
     1861 cgctctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg
     1921 aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata
     1981 tcgacgccat cgtcgagcat attcagcagc gtctcgcctc acatcaaccg cagccttctc
     2041 tgttgcacgg cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt
     2101 tcgacccggc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata
     2161 ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt
     2221 tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca aggttatttg
     2281 gcggtcagca tttggttatt gctcagcagt cattggatag attattagca gcatgatatg
     2341 ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa
     2401 gaacacataa cctgcagcaa tacaaataac cggcaggata tacagtgaga aaaactgcag
     2461 gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc
     2521 ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc
     2581 ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa
     2641 aatcacccag aacataacgt tcacgccacc attaaaattg ggcgtcgggg agttatacca
     2701 gaatagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc
     2761 gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa
     2821 agaaatcaat gtagagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa
     2881 aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt
     2941 ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg
     3001 cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa
     3061 tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact
     3121 cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct
     3181 cccgtcgtaa cgagctgccg gataccttag gtttacgcat cgatatggtg gtcgatcttt
     3241 ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg
     3301 cccgtgccat ttcactggtt gaagaaacac gtccattatt accaggcgtg cgcgaagccg
     3361 ttgcgttatg caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata
     3421 tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg
     3481 ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac
     3541 tgggcgttga cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt
     3601 ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac
     3661 gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc
     3721 tcggttaatg accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt
     3781 caactgacgg attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca
     3841 ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg
     3901 aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc
     3961 ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt
     4021 taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaagtgg atatcaaaac
     4081 cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc
     4141 tgctggcaac cttttacctt aaggttcctg aaagctggtt cattccggct gatgcgctac
     4201 aaggaatggt gctgggttat ttaagccaca tacttgccga tatgctgaca cccgccggtg
     4261 ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg
     4321 gtaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc
     4381 attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga
     4441 tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt
     4501 ctggaataag caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc
     4561 caacactatg actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact
     4621 ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc
     4681 gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt
     4741 ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac
     4801 agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt
     4861 tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca
     4921 aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg
     4981 gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag
     5041 aaactgcacg tctgaacgcc atcgaaagta actatgttgg taaagtctct gacctgagcg
     5101 ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctg accggagcca
     5161 atccgacgtc aattatcagc gtggtcattt ttgccgcatt cctcggtgta gctgcgctga
     5221 aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt aaccgctatc gataccctgc
     5281 aaagctgggt gatgaaactg gttcgcctgg tcatgcagtt gaccccttac ggcgttctgg
     5341 cactaatgac caaagtggtt gcaggttcta acctgcaaga catcatcaaa ctgggaagtt
     5401 tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg
     5461 gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg
     5521 cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc
     5581 gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgcg accattggtc
     5641 agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg
     5701 gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagct
     5761 ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg
     5821 cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca
     5881 tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt
     5941 ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc
     6001 attaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcctt actttttaaa
     6061 tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg
     6121 tccaacttat caatacattc ctggctcaat gaatgtgcct gtagcgagaa gcttaaaacc
     6181 tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagtat
//
LOCUS       DAZUGK010000233         5997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZUGK010000233 DAZUGK010000000
VERSION     DAZUGK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.235"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..1266
                     /gene="ompC"
                     /locus_tag="ACHY5W_004154"
     CDS             139..1266
                     /gene="ompC"
                     /locus_tag="ACHY5W_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS4448927.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYTSNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1378..2433
                     /gene="apbE"
                     /locus_tag="ACHY5W_004155"
     CDS             1378..2433
                     /gene="apbE"
                     /locus_tag="ACHY5W_004155"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFS4448928.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2507..3571
                     /gene="ada"
                     /locus_tag="ACHY5W_004156"
     CDS             2507..3571
                     /gene="ada"
                     /locus_tag="ACHY5W_004156"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFS4448929.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAKQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3571..4221
                     /gene="alkB"
                     /locus_tag="ACHY5W_004157"
     CDS             3571..4221
                     /gene="alkB"
                     /locus_tag="ACHY5W_004157"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFS4448930.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4297..5940
                     /gene="yojI"
                     /locus_tag="ACHY5W_004158"
     CDS             4297..5940
                     /gene="yojI"
                     /locus_tag="ACHY5W_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFS4448931.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFLLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1413 a         1510 c         1641 g         1433 t
ORIGIN      
        1 aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag aatggacttg
       61 ccgactgatt aatgagggtt aatcagtatg cagtggcata aaaaagcaaa taaaggcata
      121 taacagaggg ttaataacat gaaagttaaa gtactgtccc tcctggtccc agctctgctg
      181 gtagcaggcg cagcaaacgc tgctgaagtt tacaataaag acggcaacaa attagatctg
      241 tacggtaaag tagacggcct gcactatttc tctgacgaca agtctgtaga tggcgaccag
      301 acctacatgc gtcttggctt caaaggtgaa actcaggtta ctgaccagct gaccggttac
      361 ggtcagtggg aatatcagat ccagggcaac gctccggaaa gcgaaaacaa ctcctggacc
      421 cgtgtggcat ttgctggtct gaaattccag gatgtgggtt ctttcgacta cggtcgtaac
      481 tacggcgttg tttatgacgt aacttcctgg accgacgtac tgccagaatt tggtggtgac
      541 acttacggtt ctgacaactt catgcagcag cgtggtaatg gctacgcaac ctaccgtaac
      601 accgacttct tcggtctggt tgacggtctg aactttgctg ttcagtacca gggcaaaaat
      661 ggtagcgtaa gcggcgaagg catgaccaac aatggtcgtg gtgctctgcg tcagaatggc
      721 gacggtgtcg gcggatctat cacttatgat tacgaaggct tcggtatcgg tgctgcagtt
      781 tccagctcca aacgtactga tgatcaaaat ggtagctaca ccagcaatgg tgtagttcgt
      841 aactacatcg gtactggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct
      901 aacaacatct acctggctgc tcagtacacc cagacctaca acgcaactcg cgtaggttcc
      961 ctgggttggg cgaacaaggc acagaacttc gaagctgttg ctcagtacca gttcgacttc
     1021 ggtctgcgtc cgtccctggc ttacctgcag tctaaaggta aaaacctggg tgtcatcaat
     1081 ggtcgtaact acgacgacga agatatcctg aaatatgttg atgttggcgc gacctactac
     1141 ttcaacaaaa acatgtccac ctatgttgac tacaaaatca acctgctgga cgacaaccag
     1201 ttcactcgtg acgctggcat caacactgat aacatcgtag ctctgggtct ggtttaccag
     1261 ttctaatctc gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc
     1321 gtacaaactc agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg
     1381 gaaataagct ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat
     1441 caaaaaccac aacccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaact
     1501 atgggtacct tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag cgccgaactt
     1561 aaagaaaaga ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa
     1621 gattccgcgc tgatgcgctt taacgactcg caaagtttgt cgccgtggcc ggtaagtgaa
     1681 gcgatggccg atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg
     1741 gatataaccg tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt
     1801 caaattccga gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg
     1861 acggtcatta atcagtcgca tcagcaatat ctgcaaaaag acctgccgga tttatatgtc
     1921 gatctctcca ccgtcggtga aggttatgcg gcggatcacc tggcacgctt gatggagcag
     1981 gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac
     2041 ggtgaaggcc tgccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt
     2101 caggctgtgg tggatatcaa cggtcatggg attagcacct ctggcagcta ccgtaactat
     2161 tacgaactgg acggcaaacg tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc
     2221 gaacacaatc tggtatccgt gacggtgatt gctccgacgg cgctggaagc cgatgcctgg
     2281 gatactggct tgatggtact cgggccggag aaagccaaag aggttgttcg ccgggaaggg
     2341 ctggcggttt atatgatcac caaagaaggc gatagcttta aaacctggat gtcaccacag
     2401 tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga
     2461 tggtgaccgg gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac
     2521 atgcttaact gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacgg
     2581 cgaattcgtt ttcgccgtgc gcactacagg catcttttgc cgtccgtctt gccgcgccag
     2641 acatgctttg cgggaaaacg tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg
     2701 atttcgcccc tgcaaacgct gccaacctga taaagccaat gccaagcaac atcggttgga
     2761 taaaatcacc cacgcgtgtc gactgctgga acaggaaacg cctgtaacgt tggaagcctt
     2821 agccgaccag gtggcgatga gtccgttcca tctgcatcgg ttgtttaaag cgactaccgg
     2881 aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc
     2941 gaaaggggag agcgtgacga cgtctattct taacgccgga ttccccgaca gcagcagtta
     3001 ctaccgcaaa gctgacgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga
     3061 aaatctggcg gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga
     3121 aagcgagcgg gggatttgcg cgatattgct gggcgatgat gacgcgacac taatcagcga
     3181 gttgcagcag atgtttcccg ctgccgacag cgcgcctgcc gatctgacgt ttcagcaaca
     3241 tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga
     3301 tattcgcggc actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg
     3361 tgaaaccgtc agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc
     3421 cgttgccagc gcctgtgccg ccaacaagct ggctatcgta attccctgtc atcgagtggt
     3481 ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct
     3541 gcgccgcgaa gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg
     3601 tggcaagagc cactggcggc tggtgcggta attttacggc gttttgcttt taacgctgca
     3661 gagcaactaa tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc
     3721 acccccgggg gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg
     3781 acccatcggc aaggttatct ctattcgccc attgatccac aaacaaataa accgtggccc
     3841 gccatgccac aaagctttca tgatttatgt caacgtgcgg ctacggcggc gggctatcca
     3901 gatttccagc cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg
     3961 catcaggata aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tctgggctta
     4021 cccgcgattt ttcaatttgg cggcctgaaa cgtaatgatc cgctaaaaag attgttgttg
     4081 gaacatggcg atgtggtggt atggggcggt gaatcgcggc tgttttatca cggtattcaa
     4141 ccgttgaaag cggggtttca tccactcacc actgactgcc gctacaacct gacattccgt
     4201 caggcaggta aaaaagaata aaaataagaa ttattattgc tgtgtgcgcg aagatgttta
     4261 aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg
     4321 cggcagtatc gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta
     4381 ggcattggct taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg
     4441 ctggtgttgc cggagtttct gggattattg ctgctgttga tggcagtcac tctcggatcg
     4501 caactggcgc tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaattcatc
     4561 aagcggattc tggatactca cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg
     4621 gcgggattaa ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct gccggaactg
     4681 gtgcagggga tcattctcac tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa
     4741 atgttgctgg taacggctat ctggatggcg atcaccatct ggggcggttt tttgctggtg
     4801 gcgcgggtgt acaaacatat ggcgaccctg cgtgaaaccg aagacaagct gtacacggat
     4861 tttcaaactg tacttgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat
     4921 gtgtttaaca acctctacat tcctgatgcg caagagtatc gccatcatat tattcgcgca
     4981 gacaccttcc atcttagtgc cgtgaactgg tcaaacatca tgatgctggg cgcaatcggc
     5041 ctggtgttct ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat
     5101 tcgttgacgc ttttattcct gcgtacgccg ctgctttcgg cggttggcgc attgccgacg
     5161 ctgctgacgg cgcaggtggc gtttaacaag ctaaacaaat tcgcgctcgc gcctttcaaa
     5221 gcagagtttc cgcgcccgca ggcgtttccc aactggcaaa cgctggagct gcgtaacgtg
     5281 acgtttgctt atcaggataa cgcgttttcc gttggtccga ttaatctcac catcaaacgt
     5341 ggcgagctgc tgtttctgat tggcggcaac ggtagcggaa aatcgacgct ggcgatgttg
     5401 ttgacgggct tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc
     5461 ggcgaacaac cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg
     5521 tttgatcaac tgctggggcc ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg
     5581 ctggcgcagc tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg
     5641 aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc
     5701 gatattatcc tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt
     5761 tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa ctattttcgc catcagtcat
     5821 gatgatcatt actttatcca cgccgaccgc ctgctggaga tgcgcaacgg gcaacttagc
     5881 gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa
     5941 tattacgacg ctgacctggc ggactgctgc cgccaggtca atatgctaaa aatccct
//
LOCUS       DAZUGK010000234         5975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZUGK010000234 DAZUGK010000000
VERSION     DAZUGK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.236"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(47..460)
                     /gene="hns"
                     /locus_tag="ACHY5W_004159"
     CDS             complement(47..460)
                     /gene="hns"
                     /locus_tag="ACHY5W_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFS4448932.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1064..1681
                     /gene="tdk"
                     /locus_tag="ACHY5W_004160"
     CDS             1064..1681
                     /gene="tdk"
                     /locus_tag="ACHY5W_004160"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4448933.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(1982..4657)
                     /gene="adhE"
                     /locus_tag="ACHY5W_004161"
                     /gene_synonym="adhC"
     CDS             complement(1982..4657)
                     /gene="adhE"
                     /locus_tag="ACHY5W_004161"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4448934.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            5050..5103
                     /gene="ychE"
                     /locus_tag="ACHY5W_004162"
     CDS             5050..5103
                     /gene="ychE"
                     /locus_tag="ACHY5W_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFS4448935.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            5134..5781
                     /gene="ychE"
                     /locus_tag="ACHY5W_004163"
     CDS             5134..5781
                     /gene="ychE"
                     /locus_tag="ACHY5W_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFS4448936.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         1497 a         1345 c         1439 g         1694 t
ORIGIN      
        1 aatcccgccg atggcgggat tttaagcaag tgcaatctac aaaagattat tgcttgatca
       61 ggaaatcgtc gagggattta ccttgctcat ccatcgcttt tttgattaca gctggagtac
      121 ggccctggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta tattttgccg
      181 gacgctgagc acgtttagct ttggtgccag atttaacggc ggcaaggcta ttcagcagct
      241 cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagt ttacgagtgc
      301 gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta acaacaactt
      361 ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa
      421 gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc tcaaacttat
      481 attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca atagccagga
      541 atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct tttcataaaa
      601 ttagccagaa aagacgcggc atatagccct atttgcaccg atgatttcgc tgcacgtgag
      661 gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc tgacgacctt
      721 tcagtcttct gtattagtcg tgtttacgag aattccctat taagcgaatg atgaaaagta
      781 gaacagtcgc aataagagca gggacttagt attgcactat ctcctggagg tcaacagagg
      841 gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca tttacccgct
      901 tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg
      961 agaacgcata taccacttct gtgcatactg ttgagctgaa aaactgacga attatgataa
     1021 actccagcca actttatttc atatcattga gggcctgtgg ctgatggcac agctatattt
     1081 ctactattcc gcaatgaatg cgggtaagtc tacagcattg ttgcaatctt catacaatta
     1141 ccaggaacgc ggcatgcgca ctgtcgtata tacggcagaa attgatgatc gctttggtgc
     1201 cgggaaagtc agttcgcgta taggtttgtc atcgcctgca aaattattta accaaaattc
     1261 atcattattt gatgagattc gtgcggaaca tgaacagcag gcaattcatt gcgtactggt
     1321 tgatgaatgc cagtttttaa ccagacaaca agtatatgaa ttatcggagg ttgtcgatca
     1381 actcgatata cccgtacttt gttatggttt acgtaccgat tttcgaggtg aattatttat
     1441 tggcagccaa tacttactgg catggtccga caaactggtt gaattaaaaa ccatctgttt
     1501 ttgtggccgt aaagcaagca tggtgctgcg tcttgatcaa gcaggcagac cttataacga
     1561 aggtgagcag gtggttattg gtggtaatga acgatacgtt tctgtatgcc gtaaacacta
     1621 taaagaggcg ttacaagtcg gctcattaac ggctattcag gaaaggcatc gccacgatta
     1681 ataagaattt ctttactgac agggtgagca gggcactttt atcctgtcag ttcgttttac
     1741 gcacttcttc cgggctatac acccttctcg gcagttttta acgccgctat acgcctcaca
     1801 gggctcttaa gcaccgacgt tgacttgtga cctgtaaagt acaatatccc tgtgtttagg
     1861 cgttatacat cgtcgcaaat atgatgaagg ctaatgctgt cggtttatgg aaaagttgct
     1921 ttgggtaaac aaaaaaacgg ccccagaagg ggccgtttat gttgccagac agcgctactg
     1981 attaagcgga ttttttcgct tttttctcag ctttagccgg agcggcttct tttttcgctg
     2041 cagtttcacc ttctacataa tcacgaccgt agtaggtatc cagcagaatc tgtttcagct
     2101 cggagatcag cgggtaacgc gggttagcgc cggtgcactg gtcatcgaat gcatcttcag
     2161 acagtttatc cacgttcgcc aggaagtctg cttcctgaac gccagcttca cggatagatt
     2221 tcggaatacc cagttcagcc ttcagcgttt ccagccatgc cagcagtttc tcgatcttag
     2281 cagcagtacg gtcgcccggt gcgctcagac ccaggtggtc ggcaatttca gcataacgac
     2341 ggcgagcctg cggacggtca tactggctga atgcagtctg cttggtcggg ttgtcgttcg
     2401 cattgtagcg aataacgtta caaatcagca gggcgtttgc cagaccgtgc ggaatatgga
     2461 actgggaacc cagtttgtgc gccattgagt gacatacacc caggaaggcg ttcgcaaacg
     2521 cgatacccgc gatagtcgct gcactgtgaa cacgttcacg cgctaccgga tttttagacc
     2581 cttcgtggta ggacgctggc agatattctt tcagcagttt cagtgcctgc agagcctgac
     2641 catcagagaa ctcagatgcc agtacagaaa cataagcttc catggcgtga gttactgcgt
     2701 ccagaccacc gaaagcacac agggacttcg gcatgtccat aaccaggttg gcgtcgacaa
     2761 tcgccatatc cggggtcagc gcatagtccg ccagcgggta tttctgacca gtagcgtcgt
     2821 cagttacaac cgcaaacgga gtgacttcag aacctgtacc agaagtggtg gtgacagcaa
     2881 tcattttcgc tttcacgccc attttcggga acttgtagat acgtttacgg atatccataa
     2941 agcgcagcgc cagctcttcg aagtgagttt ccggatgttc gtacataacc cacatgatct
     3001 tcgcggcgtc catcggggaa ccaccaccca gcgcgataat cacgtctggt ttgaaggagt
     3061 ttgccagttc tgcaccttta cgaacgatgc tcagggtcgg gtccgcttct acttcgaaga
     3121 agacttcagt ttcaacgcct gctgctttca gtacggaagt gatctgatca gcataaccat
     3181 tgttgaacag gaagcggtca gtcacgatga gcgcacgttt gtggccatca gtaatcactt
     3241 catccagcgc gattggcagg gagccacggc ggaagtagat agatttcgga agtttgtgcc
     3301 acaacatgtt ttcagctcgc ttagcaacgg ttttcttgtt gatcaggtgt ttcggaccaa
     3361 cgttttcaga gatggagtta ccaccccagg aaccacaacc cagagtaagg gaaggtgcga
     3421 gtttgaagtt atacaggtca ccgataccac cctgagaagc cggggtgtta atcaggatac
     3481 gagccgtttt cattttctga ccgaagtaag aaacgcgagc tggttggtta tcctggtcag
     3541 tgtacaggca agaggtatga ccgataccgc ccatagcaac cagtttctct gctttttcta
     3601 ccgcgtcttc gaaatcttta gcgcggtaca ttgccagagt cggggacagt ttttcatgtg
     3661 cgaacggttc gctttcatca acaacggtca cttcaccgat cagaatcttg gtgttttctg
     3721 gtacagagaa gcctgccagt tcagcaattt tataggctgg ctgaccaacg atagccgcgt
     3781 tcagcgcacc gtttttcagg ataacgtcct gaacagcttt cagctcttta ccctgcaaca
     3841 gatagccgcc gtgggttgcg aaacgttcac gtactgcgtc ataaacagag tcaacgacaa
     3901 caacagactg ttcagaagca cagattacgc cgttgtcgaa ggttttggac atcagtacag
     3961 atgcaactgc acgtttgata tcagcagttt catcgataac aaccggagtg ttgcccgcgc
     4021 ctacgccgat agctggttta ccggagctgt atgcggcttt aaccatgccc ggaccaccag
     4081 tcgcgaggat caggttgatg tctgggtggt gcatcagtgc gttagacagt tcaacagaag
     4141 gttgatcgat ccagccgatc agatctttcg gagcaccggc agcgatagca gcctgcagaa
     4201 cgatatcagc cgctttgttg gtggcatctt ttgcacgcgg gtgcggggag aagataatgg
     4261 cgttacgggt cttcagactg atcagcgatt tgaagatagc agttgaagtc gggttagtgg
     4321 tcggaacgat accgcaaata ataccgattg gttcagcgat agtgatggta ccaaaagtgt
     4381 cgtcttcaga cagaacacca caggtttttt catctttata ggcgttgtag atatattcag
     4441 aagcaaagtg gtttttgatc actttatctt cgacgatacc catgccggat tcggcaacgg
     4501 ccattttcgc gagtgggatt cgagcatctg cagcagccag agcggcggcg cggaagattt
     4561 tgtctacttg ctcttgagtg aaactggcat attcacgctg ggcttttttt acacgctcta
     4621 cgagtgcgtt aagttcagcg acattagtaa cagccataat gctctcctga taatgttaaa
     4681 cttttttagt aaatcatctg ctcgaatacg agagtatagt cagtgcggtg atgatttgct
     4741 taaccgatga aaatcaaaag cttactcgcg ctcacactca ctgtgattta ctaaaagagt
     4801 ttaaacatta gagttattat ctctaatgcg tcacttccag gtggcgtaag caagattact
     4861 cacttctggg tactgattac gtgatccaaa tcaaattttt gcaaagctga cacctttcag
     4921 catcgctttt cgccattata gctaacagtt aataaattgt agtatgattt ggtggctaca
     4981 ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta agtagttagg
     5041 aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct tatttatggc
     5101 taacattatc cggcttttgc ttcggagcta accgtgattc agaccctttt tgattttccc
     5161 gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg gattattccc
     5221 gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac taaccttaca
     5281 gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga cacgattcta
     5341 caactttttg gtatatcaat tgattcattc cgtatcgccg ggggcatcct ggtggtgaca
     5401 atagcgatgt cgatgatcag cggcaagctt ggcgaggata aacagaacaa gcaagaaaaa
     5461 tcagaaaccg cggtacgtga aagcattggt gtggtgccac tggcgttgcc gttgatggcg
     5521 gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag cattagctat
     5581 ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgttgggg attgttccgc
     5641 atggcaccgt ggctggtacg ggttttacgc cagaccggca tcaacgtgat tacgcgtatt
     5701 atggggctat tgctgatggc attggggatt gaatttatcg ttactggtat taaggggatt
     5761 ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg ctccgtttac
     5821 ttcgtcatta tttttacttt gttcccgcgc agttatcaaa agcaaaagga ataggtaaaa
     5881 atattcttct caaattacag ttagttataa ggatttcctt aactgcttct cctcaccatc
     5941 atgttatttt cgccacatca taatcctggg cttgc
//
LOCUS       DAZUGK010000235         5925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZUGK010000235 DAZUGK010000000
VERSION     DAZUGK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.237"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1055
                     /locus_tag="ACHY5W_004164"
     CDS             <1..1055
                     /locus_tag="ACHY5W_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFS4448937.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAPE
                     AKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWL
                     KDFKQGPTGKESTVYGIYGISESRLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVA
                     LGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQ
                     LAELKNIEEREGRTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQAK
                     EADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPR
                     ALREYNEMVDDYNRLR"
     gene            complement(1105..2853)
                     /locus_tag="ACHY5W_004165"
     CDS             complement(1105..2853)
                     /locus_tag="ACHY5W_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFS4448938.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDTWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADNVQGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQSVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(2853..3923)
                     /locus_tag="ACHY5W_004166"
     CDS             complement(2853..3923)
                     /locus_tag="ACHY5W_004166"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4448939.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     EVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKFPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3913..5364)
                     /locus_tag="ACHY5W_004167"
     CDS             complement(3913..5364)
                     /locus_tag="ACHY5W_004167"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFS4448940.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5375..5821)
                     /locus_tag="ACHY5W_004168"
     CDS             complement(5375..5821)
                     /locus_tag="ACHY5W_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFS4448941.1"
                     /translation="MAALTASCIDLNIQGNGTYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKTSDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1531 a         1511 c         1513 g         1370 t
ORIGIN      
        1 tgaaaaggaa tttattatct tccgctatta tagtcgccat catggccctc ggtctgacgg
       61 gttgtgatga taaaaaagcc gaaacagaaa cgctcccgcc tgccaatagt caacctgccg
      121 caccagctcc tgaagcgaaa cctactgaag ctcccgttgc aaaagcagaa gctaaacctg
      181 aaacacctgc gcaaccggtg gtcgatgaac aagcggtttt cgacgaaaaa atggatgtct
      241 atatcaagtg ctacaacaag ttacagatcc cggtacagcg cagtctagcg cgttatgctg
      301 actggctgaa agattttaaa caggggccta ccggtaaaga gagtactgtt tatggcatct
      361 acggcattag tgaatccaga ctcgctgagt gtgaaaaagg cgtaaaaagt gctgtggcgt
      421 taacgcctgc gctgcaacct attgatggcg tagcggtgag ttatatcgat gctgccgtgg
      481 cgttgggtaa taccattaac gaaatggata aatattacac acaggaaaat tataaagacg
      541 atgcgtttgc gaaagggaaa acgctgcacc agacattctt aaaaaatctg gaagcctttg
      601 aacctgtagc agaatcttat catgcggcga ttcaggaaat taatgataag cgccagcttg
      661 cggagctgaa aaatattgaa gaaagagaag ggagaacatt ccactactac tctctggcag
      721 tcatgatttc agcgaaacaa attaataacc tgatatcgca agatgagttt gatgcagaag
      781 cggcaatgaa gaaagtgtct gaactggaaa cgctggtggc gcaggccaaa gaagcggata
      841 aaggcggcat gaatttctcg tttattaatt cggcaggcca gtatcaactc gaggctaaaa
      901 aatacgttcg ccgcatcaga gataaagtcc cgtactctga ctgggataaa gagcaacttc
      961 aggacgcaaa ctcaagctgg atggtcgaag actctttccc gagagcatta cgcgagtaca
     1021 acgaaatggt cgatgattat aatcgcctgc gttaaacgtt tttgatgata aatgcagtgt
     1081 cggatgcggc gcatccgaca cagttcaccc cttgtgaagt aactccataa atgcctcata
     1141 tcctgttaac ccggcaataa cctctgccgg atgctgacac aatatgcttg ccagatagct
     1201 aaatattttt agctcatgac gcgcatcggc ggcggcgcag gcgatcaaga catggttaat
     1261 gacttcgtta ttcacctcaa ctgattgggc gagggtaata aaaaatccac gaaaacgtcg
     1321 ctcctgttcg ctccagcaat gtgggatggc gaggcgatta acaatcaggt tttcaccttc
     1381 gccttcgcgg gcgatgatcc gttgcgcttc atcggcggta atatggtgtt gtgcgactaa
     1441 ttgcgcacaa atttgccggg taatatgttg ccagctttca ccgcgtacat tcgaataatg
     1501 aaaacttccg ggggcagaga aaaaacgctc cggttgctgg cgaataaatg ccgtcgccag
     1561 aaaatgtttt atttgttcga taccggcagc cgtaatgata ttttttacgg tgatgcaatt
     1621 atttaccgcg tcatccagta aataatggct gttgttaatg atcaataacg gctcaatctc
     1681 ttcgcgaatg gcaacaagct cgcttaagct acgggcaata atcacccgac aatgtaaaac
     1741 atcgcgctca atggcgagct gattaatggt ggcaatcgcg ttctggtccg agagcaaaat
     1801 gatcggctgg cgttcgtttt gatgccgttc cagcgcacag gcaaaataca aaccgatcag
     1861 atcgctatcg aaaaggggaa tatcgagctg ttcacgcagt agcgtaataa agtgcagact
     1921 catatcaaag gccgcaggcc aggcggcttt caggttattc atgctgcttt ggcggtgctc
     1981 agcaatccac actggtgcgg caaggcaacg taataaatgc ccggtaatat tctcaaccag
     2041 ctgcgtatca ataatattca atgacgcgat gcccggcacc ctttgcagca acccctgcac
     2101 attatcggca gaaattacct gtgcttgctg atgctgtttc tctatcaaac ccgtcagggt
     2161 tcttacagca ttatcaccaa gaaacaggcc gctctgttca acgatttgtt ttatctcgtt
     2221 accgggatat tgcggccatg tgtcagtaag ctgattacgc agggcgtaaa tcgccagaat
     2281 caggctggag agataatcac cctgcatcag cggccagcgg tgttggttgt cgcagaccgt
     2341 ggataattgc tgaaggttgt ctcgtgttac gcccgccagc ggtattaaaa acggatcttt
     2401 gcgtagcaag ttcgccagca agataacgcg cttctcttct gtctcatcaa tgaaatggcc
     2461 caaaccgggg cgactggcca ggcagcaagt acgttcataa cgctgtttca ggcggggcag
     2521 acgctctgct acccacgttt ctggcaaatt aagcgccgag gcgagttgcg cacggggagt
     2581 gaaagtattc agtaataaca gcgccagcag ccgatcgtcg ttatcgtgct tttgcagcaa
     2641 ctgaaaaaaa ctgcggcgct cgaagatttc cagctgatag cccgcactgc cactggcgaa
     2701 aatgcgggct ttgccgttaa gggtgaagtt gagatagtca atatcacgca ggatggtcct
     2761 gcctgaaacg ccagtctgtt gcgccagctc gccaggcgtg cgcggctgtt gctccagtag
     2821 atcgacaatc tttaactggc gttcgtttag cattaatcca cctgacggaa ctgactaaac
     2881 gcgttgactt tctccgcggt taaggtcgtc agaaaacccc gtatcagcgt gagcagagca
     2941 tcgtaatcgg cttttgaaat catgccgctg ttggcgtgca gataacgagt tggcagacac
     3001 agcgcgacaa ccggacgccc gccgcccatc acgttgtagc gcccgccatc cgtcgcaccg
     3061 gttttcatgg tggaaaattg cagcggtaaa tcgttatgtg cggcacagct ttttaacgcc
     3121 gccaccagtt tctggttggg gaagtagcgc ttgtcaaaca gcatcagccc cggcccctgg
     3181 cccagtttca gcgggaattt tatgttatca atgcccggaa catcgcccgc tacggcggta
     3241 tccaacacga tcacgacgtc cggtttaatg tgttccgccg aggtttgcgc cccgcgtagc
     3301 ccaacttctt cttccacact gccaacgcca tacagcgtaa tttcgggatt attcaccgtc
     3361 tgcaatagtt cagccatcat tgcgcagccg atgcggttat ccaacgcctt gccgaccact
     3421 ttatcttcgc cccagcaggc aaaattggct tccgggctaa taaaattacc aatttccacg
     3481 ccgcgttttt ccgcttcttc gcgactgttc gcgccaatat caatgaacat ctcatcaaat
     3541 gacagcggtt gttgcttttg cttttccgtt aacgcatgag gcgctacgga accaatcaca
     3601 ccagggattt tcacttcctt gtgtgtgcgt attgttaccc ggtggttgag catcgactga
     3661 ttccaccagc caccaatagt ggtaaagcgc agaaaaccgc tctcgtcgat gtgggtgacc
     3721 ataaagccga cttcatccat atgcccgaca acggcaactt ttggaccttt attcccctta
     3781 cgggcaacaa agctgcccag accatcaaag gtgatctcat taacgcaagg ttccagcgtg
     3841 tttatcagaa tgtcgcgaac ttcctgttca tcgccgctga cggcgctggc ttcgcacaac
     3901 tgttgcagta actcaatgtt catggacaag ctcctgttgc gcggctttcg ctttgcggcg
     3961 taaccacaat cctttcagga cgataatgaa tgaaatgttc agcgcgaggc caatcgccag
     4021 caccagataa aacgaaccta ccggcgacat caggccgatc agcgggtcaa agatacccaa
     4081 accaggtgcc agacgtttga ttccgaaagc gatcaccagc acgccggtaa taccgcccgc
     4141 cagcgtattg gcggtaatca tcggcaacgg agctgccaga gcgtaaggaa tagccggttc
     4201 ggttgcgact gtggcaccga ccacaatggc actactggca gcctcttttt cctgtcgggt
     4261 aaacagtttg ggcgcaatga acgtcgcaag acctgccgcg acgggcggca tcagcgcgac
     4321 aacgccgacg atggcgtacc agtcataaat gtgtttttcc agcagtgaga agcagaagaa
     4381 ccaggcggtt ttattgatgg ggccgcccat atcgaacgcc agcatcgcac cgaccagaaa
     4441 cgctgccgca aacttcattg atggaggaat ggtattgagg aagtgcagta gcccgcccat
     4501 gaggtcggac atcaccggtc cgataacgta gtaagtgagc acgccgaaaa ccagcagagt
     4561 gacgaacggg atcagcattg aaccgagcag cggttgtaat gctttgccaa gacggacttt
     4621 acggaaccag aagacgaaat agccaatcgc cagccccagc accaccgcgc cgaggaatcc
     4681 cgcacccgac tgggtgccga gcagcgcttt gtcgttcgcc agatagcaaa ccagaaacgc
     4741 cggagcgaaa gccggtttat cggcaatcga cgaggcaata taagcgccca tgatggggat
     4801 cataaaggtg aagccaaggt aaccgatgga ttcaactacc caggtaaacg acggcgcgcc
     4861 tttcgacata tcggtatacg gcaaaccgaa ctgcaccagc atgttggcaa gcgccaccag
     4921 gataccaccg ccgatgacaa acggcagcgc cgcagagacg cccgccatca ggtggctcat
     4981 cacgctgccg ctttgtactt cctgtttgcc tagcttcacg ccgctatctg cggcaaaaag
     5041 ctgcgaattt gtcggtaatt cactgaaaat ctggtcgatg tttttcagcg cttgggagat
     5101 ggcaatctca taaacctttt tcccggcaaa ccgcgcgcga tcgtcaccgc tcaggccacg
     5161 cccggtggcg agaatcacgt aatcggcagc ggcaatctct tcgctggata agcggttttc
     5221 aacgccgctg gacccttgag tttccacttt tatggtatgg ccgagagaac gggctttctg
     5281 ttccagggct tccgccacca tgtaggtgtg agcgatcccg gcggggcagt tggtgatcgc
     5341 gacaatacgt aaggatgact ccatcgccac ttccttatga gctgagggtt tgatttaaca
     5401 aggcaagcac ctggtcggcg tcgccctgtt gcagttgatg aataaaatcc tggtggataa
     5461 ttttgcgaca cagtgtgccg atgattttga cctgatcctc ttcgccgctt tgcggcacgc
     5521 cgaggcagat ccagcaattg acgtcttcgc catcgctggt tttccagtca atagcctgcg
     5581 ctttgcgggc gaataacacg aacggttgtt taacgcaggc gcttttaccg tgcggcacgg
     5641 cgacaccaga accaaaaccg gtggagtgca ttttttcgcg cagcagcagg gtttgcagga
     5701 actggtgtga gtcggtgata aaaccgtttt gtaacgccat tgtcgctaac tgtttcagaa
     5761 tggaataagt gccattgccc tgaatattca ggtcaataca gcttgcagta agagctgcca
     5821 ttaatcggac tccatgaaaa aatatttacg ttaattgtaa tagcggggcg ggtttgtgat
     5881 gagcgcggat atgtcgtgaa gtcgtgacaa tataaagcag agtga
//
LOCUS       DAZUGK010000236         5892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZUGK010000236 DAZUGK010000000
VERSION     DAZUGK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.238"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            34..759
                     /locus_tag="ACHY5W_004169"
     CDS             34..759
                     /locus_tag="ACHY5W_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4448942.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYYLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            717..1394
                     /gene="bglJ"
                     /locus_tag="ACHY5W_004170"
     CDS             717..1394
                     /gene="bglJ"
                     /locus_tag="ACHY5W_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFS4448943.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1432..2220)
                     /gene="fhuF"
                     /locus_tag="ACHY5W_004171"
     CDS             complement(1432..2220)
                     /gene="fhuF"
                     /locus_tag="ACHY5W_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFS4448944.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNVTPHSPQHRMETL
                     ISQALVPVVLALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2361..2597
                     /locus_tag="ACHY5W_004172"
     CDS             2361..2597
                     /locus_tag="ACHY5W_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFS4448945.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
     gene            complement(2636..2722)
                     /locus_tag="ACHY5W_004173"
     tRNA            complement(2636..2722)
                     /locus_tag="ACHY5W_004173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2686..2688),aa:Leu,seq:cag)
     gene            complement(3023..4054)
                     /gene="rsmC"
                     /locus_tag="ACHY5W_004174"
     CDS             complement(3023..4054)
                     /gene="rsmC"
                     /locus_tag="ACHY5W_004174"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFS4448946.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4157..4570
                     /gene="holD"
                     /locus_tag="ACHY5W_004175"
     CDS             4157..4570
                     /gene="holD"
                     /locus_tag="ACHY5W_004175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFS4448947.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4539..4985
                     /gene="rimI"
                     /locus_tag="ACHY5W_004176"
     CDS             4539..4985
                     /gene="rimI"
                     /locus_tag="ACHY5W_004176"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4448948.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5000..5677
                     /gene="yjjG"
                     /locus_tag="ACHY5W_004177"
     CDS             5000..5677
                     /gene="yjjG"
                     /locus_tag="ACHY5W_004177"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFS4448949.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVAFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
BASE COUNT         1469 a         1451 c         1532 g         1440 t
ORIGIN      
        1 tcatgattta tagacagtgg atgtggagga aatatgttgc caggatgctg caaaaatgga
       61 attgttatca gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat gaggactcac
      121 ttccctgaat atgaaataat atccagcgcc tctgcggagg accttacctt attacaatta
      181 cgtcgttccg gattagtcat tgctgattta gccggtgaaa gtgaagatcc acgttctgtt
      241 tgtgaacatt attattattt aatctcacaa tatcgggaaa ttcactgggt tttcatggtg
      301 tcacgctcct ggtattccca ggcagtagaa ctgctcatgt gccctacggc gacgttattg
      361 tctgatgttg aacccattga gaatctggtc aagaccgtac gttccggcaa tacgcacgca
      421 gagcgtatca gcgctatgct gacttccccg gcaatgactg aaactcatga ttttagctat
      481 cgctccgtca ttctcactct ttcagagcgc aaggtactgc ggctattagg taaaggatgg
      541 ggcatcaacc agatagcttc attgcttaag aaaagtaata aaactatcag cgcccaaaaa
      601 aacagtgcga tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc atggataaat
      661 agcgcgcagg gtgcaagaga acttaacttg ccttctgttt atggagatgc cgcagaatgg
      721 aacacagccg aattaagaag agaaatgtcg cactcataga aaaatgcgtc atgagtagta
      781 tcggtattga gagtttattc agaaagtttg cgggtagccc ttataagctc catacctata
      841 ccagtcagga gtcatttcag gatgccatgt cgcggatctc gtttgcggcg gtcatttttt
      901 ctttttctgc catgagaagt gagcgcagag agggattatc ttgcctgact gaactggcga
      961 ttaagtttcc gcgtacccgg cgtttagtta ttgcggatga tgatattgaa gctcggctga
     1021 ttggttcatt gtcgccatca ccgctggacg gtgtattaag taaagcgtca acgctggaga
     1081 tttttcatca ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc gaccgactga
     1141 acaatcagtg gtacattaac caaagccgga cgctaagccc gacggagaga gaaatattgc
     1201 gctttatgtc gcgtggctac tcaatgacac aaattgccga gcagcttaaa cgtaatatca
     1261 aaacgatccg cgcgcataaa tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag
     1321 ggttgttgga ggccgcagat attctgttat gtatgcggca ttgcgaagca agtaatgtgt
     1381 tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct ttcatttcag
     1441 cgtacaatcg ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg
     1501 caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt caccggtagt
     1561 aagcgttttc tcaaaaaaga gggcatggcg cagcgattca accgtcgcct cgccgagcag
     1621 ttgcttcatc tcagtaagat accagttgat caaataaccg gtattactcc agataagttt
     1681 gccgttaatc tctcccgttg cttctagtgc cagcacaacc ggaaccagcg cctggctgat
     1741 taacgtttcc attcgatgct gcggcgaatg tggtgttacg tttttatctt cacacacatc
     1801 gacccagaaa caggcgacgc gtccggtttc gtgaaactca gcatggaaat gttccggcga
     1861 cacatctaat gctttttcct gcgtcagtag cgccagcatt aatggtggta ccatcaggcc
     1921 gatataccac tgcgcccaca gtgagatcag cggtttgttc tcgcggatca tcgttggttg
     1981 gttgcgatag atatgatcgg aatagaccgc cagtagagaa cttagagcat tcggtgatga
     2041 ccattgcgcc agcgtcatgg cgttaagcgg agcgggttca tccaggcgga taaactccag
     2101 caaatgttca cgatgttcgg cgatcatcgc ccgtacggct tgtgcaagcc cagcatcctg
     2161 cggctggaga tgtgttcgcc agatgacatc ttcatagagc ggtgcggaac gataggccat
     2221 aatcgggata gtaatctaaa tgataatgat tgctaatcct agcgataggt ttacccgata
     2281 gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg gttatcatta
     2341 tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc
     2401 tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat cagtggcgga
     2461 ttgtcattct gatgggattg atattgacgc cggtgatgct gtatcacaaa cagttacggc
     2521 attacgtttt gctaccatcg tgcctggcgc ttagtgctgg catcatgctg atgctaatga
     2581 atttgaatca gggatgaaaa atcaaggaag aaacaagaaa ggaagtaaag ataattggtg
     2641 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
     2701 ctaccaattc cgccaccttc gcataccatc aattcttaaa aagaattgct accacggagg
     2761 cgcattctag tggttttcag cttttcgtca atagttaatt atcgacagag gtgtaattgc
     2821 tggaaaaata tccatcagga gactaacgtg caggttcggt aggtcggata aggcgttcac
     2881 gccgcatccg acacgatatt cgggggcaga tgccagatgc gacgctggcg cgtcttatct
     2941 ggcctacgaa gggctaacgt gcaggttttg taggtcggat aaggcgttca cgccgcatcc
     3001 gacacggtat tcggcgagat aattaacctt tcttcgcctg gcgggtcata atggcgcgat
     3061 acaccttgaa gcgcccggtt tgcgcgataa cttcatggaa gccaaatgtc tcatccagca
     3121 cgtccgggta aggcaggaag gcgttcgcta caattcgcag ctcgccgccg ctattaagat
     3181 gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag gctggtttgc atcccatcgt
     3241 ggaacggcgg gttggagatg atcatatcaa aacgaccttt cacctcggaa aagacgttgc
     3301 tggcaaagac ttcaccttca acaccgttgg ccgcaagcgt tgcacggctg gcttctaccg
     3361 ccggggcaga gacatcgcac agagtgagac gaattttcgg cgaatggcgc gcaaaggcaa
     3421 ccgaaagcac gcccgcgcca cagccgacat ccagcacttt acctttcgtg tgcggagtta
     3481 acgttgagag cagcaactgg ctaccgacat ccagaccgtc gcggctaaac acgccaggca
     3541 gcgttttaac cgtcaggcca tcgacgctgt attcgcccca gaatttatcg gcatcaaata
     3601 ccggctgttt ttccagacga ccaaaataga ggccacagcg acgagcgctg tcgactttat
     3661 tcaacggcgc ataatctgcc agcatctgct cggcgctgcg cacgccgctg cggttctcgc
     3721 caacgacaaa aatatctgtc cccactggca gcagagaaag taaattcatc aactggaact
     3781 gggcttccgg tttgttcttc ggccagtagt aaatcagcgt atcgcaatct gcgacatcat
     3841 ccgccgtggc gaccaggcta aaacgggcgt tatcccccat ctggcggctt aacacctgcc
     3901 aatggtggaa ttgctgggta tgagcacggc tggccgcggt atctaaacgc gcgggcaggt
     3961 catcctgtaa gtctccggca aacagaatac ggctttgttc gaaatcatca ctgtgacgca
     4021 gcaagacttc acttgccggg gtaaatgcag acatggaatg ctcctcaatt gatactggcg
     4081 gcgattatag ccatatgttg gcgcggtatc gacgaatttg ctatatttgc gcccctgaca
     4141 acaggagcga ttcgctatga catcccgacg agactggcag ttacagcaac tgggcattac
     4201 ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggag attgccattg cgatcccggc
     4261 acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc ctgactgatc ctttagtgag
     4321 cgatgttctg cgtgcattaa ccgtcagccc cgatcaagtg ctgcaactga cgccagaaaa
     4381 aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg cggttgggta ctgacgaacc
     4441 gctatcactg gaaggcgctc aggtggcatc accggcgctc accgaattac gggcaaaccc
     4501 aacggcacgc gccgcgttat ggcaacaaat ttgcacatat gaacacgatt tcttccctcg
     4561 aaacgactga tttaccagcg gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga
     4621 gtgaaaaaac ctttgccagc aaccagggcg agcgttatct caactttcag ttaacgcaaa
     4681 acggcaaaat ggcggcgttt gcgattacgc aagtggtgct ggatgaagct acattgttca
     4741 atattgcggt cgatcctgac tatcagcgtc agggattggg aagggcgctg ctggaacatc
     4801 tgatcgacga actggaaaaa cgcggcgtgg cgacactatg gctggaagtc cgtgcctcaa
     4861 atgctgccgc cattgccctg tacgaaagtt taggctttaa cgaggcgacg attcgccgca
     4921 attactaccc caccacggac ggtcgcgaag acgccatcat catggcgttg ccaatcagta
     4981 tgtaagacaa ggtggaataa tgaagtggga ctggattttc tttgatgccg atgaaacgct
     5041 gtttaccttt gactcattca ccggcctgca gcggatgttt cttgattaca gcgtcgcctt
     5101 taccgctgaa gattttcagg actatcaggc cgttaacaag ccactgtggg tggattatca
     5161 aaacggcgcg atcacttcat tacagcttca gcacgggcgt ttcgagagct gggccgaacg
     5221 gctgaaagtt gaagcaggct tgcttaacga tgcctttatt aatgcgatgg cggaaatctg
     5281 tacgccgttg ccgggcgcgg tttctctgct taacgccatt cgtggcaacg ctaaaatcgg
     5341 catcatcacc aacggtttta gcgccttgca gcaagtgcgt ctggaacgga cgggcctgcg
     5401 tgattacttc gatttgctgg tgatttccga agaagttggc gttgccaaac cgaataagaa
     5461 aattttcgat tatgcgctgg aacaggcggg caatcctgac cgttcacgcg tgctgatggt
     5521 tggcgacact gccgagtccg atattctcgg tggcatcaac gccgggcttg cgacctgctg
     5581 gctgaatgcg cacaatcgcg agcaaccaga aggcatcgcg cccacctgga ccgtttcttc
     5641 gttgcacgaa ctggagcagc tcctgtgtaa acactgattg cctccccccc gttgatgggt
     5701 aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga
     5761 aagaattatg acgttgtctc cttatttgca agaggtggcg aagcgccgca cttttgccat
     5821 tatttctcac cccaataatt aagcctaagt taaagctctt ttactttttc aaagtccttt
     5881 cagttaattg ag
//
LOCUS       DAZUGK010000237         5883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZUGK010000237 DAZUGK010000000
VERSION     DAZUGK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.239"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2085)
                     /gene="pqqU"
                     /locus_tag="ACHY5W_004178"
     CDS             complement(<1..2085)
                     /gene="pqqU"
                     /locus_tag="ACHY5W_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFS4448950.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFDSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNI"
     gene            2327..3388
                     /locus_tag="ACHY5W_004179"
     CDS             2327..3388
                     /locus_tag="ACHY5W_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFS4448951.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3501..5000)
                     /gene="ansP"
                     /locus_tag="ACHY5W_004180"
     CDS             complement(3501..5000)
                     /gene="ansP"
                     /locus_tag="ACHY5W_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFS4448952.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5267..>5883
                     /locus_tag="ACHY5W_004181"
     CDS             5267..>5883
                     /locus_tag="ACHY5W_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFS4448953.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNVII"
BASE COUNT         1553 a         1506 c         1419 g         1405 t
ORIGIN      
        1 gatattcacg ccgacaccat aatttcgtcc gggcgcaggt tcgtaatatc gcccgtttga
       61 ctcattgaca atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa
      121 taaatccaca gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt
      181 ataagacggc gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc
      241 ttccgtgcct gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt
      301 acgggcgatc cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg
      361 ataggtcgca tcaagccagg tccaggacgc gtttacgcga aaatctcccg cgaaacgttg
      421 atcccatgcc agttcagcgc cttgacgacg ggtctttcca gcatttttgt aagtcgtacg
      481 cccaccgcta ctgctatcga caacaatttc atcatcggtg tcggtctgga acaatgcgag
      541 actaagcagc ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga
      601 tggttttaaa ccaaagttca taccgctttg cccatcagcg cgataagaca gctcattaat
      661 cgtcggcgtt tcaaaaccac gcccggctgc cagatagata ttccaggcat cggtcattgc
      721 atattttaac gatccggctg gtagccattt gtgataactg gcatcaccgc tgtcatcgcc
      781 gttacccgga gtaatgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc
      841 agcatccagc gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt
      901 ccacatcaga ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc
      961 gctattcagg cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag
     1021 gccggtagtg aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat
     1081 tccctggtaa tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg
     1141 tgccatgggt attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact
     1201 catatcatcc cgcgaactca ggctacgttc atagcgcaac ccagcctgag tttgcttgat
     1261 ggtttttcgc gtgtcgtact gttctgcacg aggcgcttgt tgtggattcg ccttccattc
     1321 tgctttggtt agcccacctg ggtcatctgc tttgatatcc acactattga aaatcaggct
     1381 taatttgctg gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc
     1441 gccactatgg tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcaac
     1501 atcgccgggc tgtgtgccgt ctcccgttgc acccgttgct ttcagcccat agcgccagct
     1561 gtcaaaactg ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt
     1621 ggtgacattc attacaccgc cagacgcgtt gccatacagg gcagagaagg ggccacgcag
     1681 cacttccaca ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg
     1741 catggtggcg ggaataccgt ccacatacag gcgaataccg cgaattccgt aagtggaacg
     1801 ggagccaaat ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac
     1861 ctgcaaacca ggcacgccgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg
     1921 catctcctcg ccatccacca cgcttactgc tgcaggggta tccagttctg aaaccacctg
     1981 cggcgcggca ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaaggac
     2041 aagcagtgcg ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt
     2101 aagaatggga aaacgccgtc atggtaatga aattgtaaat ttatggaaaa tgaaacggca
     2161 caatacgtta agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga
     2221 gaaaataatg attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga
     2281 agcattgtga agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca
     2341 tctgttttca ttgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc
     2401 attcagtacg caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga
     2461 aatggcttat agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact
     2521 ggataaaggt ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat
     2581 ccataacgat ctcaagccgt ttggtgccac catcaataac acgactcaga cgttgtggtt
     2641 tggtaacacc gtaaacagcg cggtcacggc gatagatgcc aaaactggcg aggtgaaagg
     2701 acgtctggtg ctggatgatc gtaagcgcac ggaagaggtg cgcccgctgc aaccgcgtga
     2761 gctggtagct gatgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt
     2821 gatttgggtc gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa
     2881 aatgagtact ggtctggcgc tggatagcaa aggcaaacgt ctttacacca ctaacgctga
     2941 cggcgaattg attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct
     3001 ggatgacggc aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc
     3061 atttatcacc gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat
     3121 tctggcgaag gttgcggcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga
     3181 agcctacgta acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa
     3241 agtggtgaaa acgttcgata cgccgactca tccgaacagc ctggcgctgt ctgccgatgg
     3301 caaaacgctg tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc
     3361 ggacgatgtg attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt
     3421 gcctgatttt tatcacgccg catgcggcac cttcgtcagt aatctaaatt tgcccggcac
     3481 gtagccgggc aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt
     3541 tcttcatctt cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca
     3601 ccaaaccagc ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc
     3661 ccgttcgggt aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc
     3721 caggaagtga agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta
     3781 atcgctttac gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc
     3841 agcgacgcga agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat
     3901 acgccgacga catacacaac caatgtcgcc agaatcccgg catacggcac atgctgacga
     3961 ctcattttcg ccataaaact cggtgcggaa ccgcccatcg ccattgagcg cagaatacgt
     4021 ccggtgcagt acagacctga attcaggctg gagagggcag cggtcagcac cacaatgttc
     4081 ataatgctgc cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc
     4141 cccgcctgat acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa
     4201 aacaggccaa tacgccaaat cacactgtta atcgctttag gcaccatggt ctgcggatct
     4261 ttacattctc cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc
     4321 tgaatcaaca ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt
     4381 aaatgaaagc cagtggtgtt gccatccagc ggctgaccac taccgaggaa caccgtaccc
     4441 acgaccaaaa aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc
     4501 gcaaaccatt tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca
     4561 aagacccact gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg
     4621 gtaatatcaa caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa
     4681 gcggctttct cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg
     4741 tgtagcacca gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa
     4801 taaaccagtg ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag
     4861 ccggtgccaa tcgcgccgcc aatggcgatc atctgtacct gacgattgcc catcgctttg
     4921 tgatacccct cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg
     4981 tcggtgtcgt gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt
     5041 gccatgaaca atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt
     5101 ataaaactat cggcgcagca tcctacccgt tatcgataaa cgatgcaaaa catcccctta
     5161 caatcctgaa ggggattaat acaactgacg aaaaaatgac aaatcctttt gctggttaac
     5221 ctgtgtactg tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta
     5281 tggcgtaccc ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc
     5341 ttatcagttc gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa
     5401 aaccctgaac ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac
     5461 ggagacagcg gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt
     5521 tgggcgcgcc gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta
     5581 tccaacattc actttcgccg attaccctga gcgttgggct cctgacgcac ccgaacagtt
     5641 aaagaagaat gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac
     5701 cgctgaacct tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat
     5761 gcgcacatgg gggcctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat
     5821 tgctgatgct gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgtcattat
     5881 tta
//
LOCUS       DAZUGK010000238         5834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZUGK010000238 DAZUGK010000000
VERSION     DAZUGK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.240"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..735
                     /gene="gadW"
                     /locus_tag="ACHY5W_004182"
     CDS             7..735
                     /gene="gadW"
                     /locus_tag="ACHY5W_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFS4448954.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1098..4211)
                     /gene="mdtF"
                     /locus_tag="ACHY5W_004183"
     CDS             complement(1098..4211)
                     /gene="mdtF"
                     /locus_tag="ACHY5W_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFS4448955.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4236..5345)
                     /gene="mdtE"
                     /locus_tag="ACHY5W_004184"
     CDS             complement(4236..5345)
                     /gene="mdtE"
                     /locus_tag="ACHY5W_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFS4448956.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5453..5731
                     /locus_tag="ACHY5W_004185"
     CDS             5453..5731
                     /locus_tag="ACHY5W_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448957.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5732..>5834)
                     /locus_tag="ACHY5W_004186"
     CDS             complement(5732..>5834)
                     /locus_tag="ACHY5W_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS4448958.1"
                     /translation="SQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1413 a         1472 c         1488 g         1461 t
ORIGIN      
        1 ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga tatttatcat
       61 gaacagcaaa aaatatcgct gcataacgag agtatcctgc tgctggataa aaatttggca
      121 gacgattttg cgttttgttc accggatacg cgacggctgg atatcgatga gctgacagtt
      181 tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca tagcaaagac
      241 cgtctgttaa ttaaccagtc accccccatg ccgctggtga cggcgatttt tgatagtttc
      301 aatgactccg gggtaaattc gccgatactg agcaaaatgc tctatctttc ctgtttatcg
      361 atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag tactgtttca
      421 ggaaaagttg aacgccttat tagctttgat atcgctaaac gttggtatct gcgcgatatc
      481 gcggaaagaa tgtataccag cgagagtcta atcaaaaaaa agttgcagga tgaaaatacc
      541 tgtttcagta aaatattact cgcctccagg atgtcgatgg ccagacgatt actcgagtta
      601 cgtcaaattc ctctgcatac tattgcggaa aaatgtggct atagcagtac atcgtacttt
      661 ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca acattcgcca
      721 ggtacctttt cctgacatat ttcgcatttg aatattggtc aggatctcac acctgcttca
      781 tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt tcgcagtttg
      841 ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat tattaagctg
      901 acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat
      961 ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct gacggttagc
     1021 tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc acgttcaacc
     1081 ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc cactacaacg
     1141 aaaaagaccg gaacaaagta aattgccagc actgttgcgg caaacatccc gcccatcacg
     1201 ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac cagcggcagc
     1261 acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat ccgcgccgct
     1321 tcgatgattg cctctatcgg cgttttccct tctttctgca tcatctcaac ggcaaattcg
     1381 acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag
     1441 tagacgtcat tacttaagcc gcgcagatcg gtggcgagta atgcgccaac gacgcctaac
     1501 ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc ggcgagggcg
     1561 aggaacacca cgaccagtga aatggcatat agcgcgggag cctgatttga ggataacgct
     1621 tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt agcgaccagg
     1681 tctgccataa atttcattgc gtcaccggta cttttcccgg ccgccgcttc acctaaaatc
     1741 tccattgacg ggatgccgtt gtagcgttcc agtcgcggtg aaccataggt ccattcggta
     1801 gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata ccattggttg
     1861 atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt tttcacccgc
     1921 ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat tgtctggttg
     1981 atatcagaca gcgccacgcc cattgcttca gctttcgcag cgttgacgtt cactttgaac
     2041 atcggcgtat cttccaggcc gttcgggcgt accccggtga cctgattcgg tgattgcgct
     2101 gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag gttaccgttg
     2161 tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc gggtaagttg
     2221 aacgggaaga cgacggcttt attgatactg cttagcgcaa tcattgcccg ctgaatgatc
     2281 gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact gataaacgcc
     2341 aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa caccgactgg
     2401 acattatctt tctctttagt cagataataa tccgtcacct gttgcagcac tttcgtggtg
     2461 ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc ctgatcctct
     2521 tctggtaaga aagaggtcgg cgtgcgcagg aacagcaccg ccatcccggc gcaaatcagc
     2581 aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct atcggtatag
     2641 tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt aggtttgtga
     2701 ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggttag gctcattgcc
     2761 acaaatactg aaagcagcat ggaggagatc agcgtgatgg aaaactggcg gtagatctcc
     2821 ccggttgcac cgctcataaa ggccatcggc ataaacactg cggaaagaac aacggcaata
     2881 ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt cggcggtagc
     2941 ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc atccaccagt
     3001 aaccctatcg ccagcaccat cccgaacata gtcaacgtgt tgatggtgaa accgaccgcc
     3061 gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg gatgattgtg
     3121 gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat agcctcaacc
     3181 agtgttttga aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa
     3241 accgtcttca gacttgccgg gaaataggct gataagcggt tcagttcctc tttgactgcc
     3301 cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc ggctgcaggt
     3361 ttgccgttat agcgcgccac ggtggaataa tcttccgccc caagttcgac gcgggcgaca
     3421 tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt gccaaattct
     3481 tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagctgctg gtctgccgcc
     3541 tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt aatctgggaa
     3601 ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag ccagatacgc
     3661 atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg
     3721 tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct gccgttatca
     3781 gaaatgaacg ccgctaccat caggatatta ctgctcgatt tatcgacgct aatcccctgc
     3841 tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg cacctgaacc
     3901 tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc attacccgcc
     3961 gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt ttgctcaatc
     4021 acctgagtga ccgagtcttc taccgtttgc gcatcggcac ccggataggt agcgctgacg
     4081 gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga
     4141 ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg atcaataaaa
     4201 tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc ggtgctggcg
     4261 ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg
     4321 gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg gtcgccgatg
     4381 gctttgctgg cttcaatttc gcgtagctgc acgacatcgt ctttatccag aatgagcgcc
     4441 gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg
     4501 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
     4561 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
     4621 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
     4681 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
     4741 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
     4801 gaatctgcct gattagcggt aacgagagcg ccgacggtca ccgacgattt cccgctgacg
     4861 cccgtaatcg gcgaggtgac attcgcgtat tgcaggttga tcgtcgcctg ttcaacagcc
     4921 gctttggcga cggtgacatt ggcttctgct tcattcaact gggtgcgcgc ggtgtcgtaa
     4981 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
     5041 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
     5101 tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg
     5161 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa
     5221 ggaacggttc taccgggcaa ttcgctcaac acattgaccg acccagggga gagtgtgacg
     5281 acaccgacct caggcgtcat ggcggcggtg ttttccgccg atttgtcatc gcaggcggtg
     5341 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttattc
     5401 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
     5461 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcatagtga
     5521 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     5581 cattcgggca acgaagggtt cgactggtgg atacacatac caggggaata aagtcgtttt
     5641 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     5701 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     5761 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     5821 atgtttgttg cgaa
//
LOCUS       DAZUGK010000239         5804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZUGK010000239 DAZUGK010000000
VERSION     DAZUGK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.241"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..112
                     /locus_tag="ACHY5W_004187"
     CDS             <1..112
                     /locus_tag="ACHY5W_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS4448959.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHY5W_004188"
     CDS             171..374
                     /locus_tag="ACHY5W_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS4448960.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..1424
                     /locus_tag="ACHY5W_004189"
                     /pseudo
     CDS             374..1424
                     /locus_tag="ACHY5W_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1541)
                     /locus_tag="ACHY5W_004190"
     tRNA            complement(1452..1541)
                     /locus_tag="ACHY5W_004190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Ser,seq:cga)
     gene            1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHY5W_004191"
     CDS             1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHY5W_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFS4448961.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2533..2608
                     /locus_tag="ACHY5W_004192"
     tRNA            2533..2608
                     /locus_tag="ACHY5W_004192"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2566..2568,aa:Asn,seq:gtt)
     gene            2770..4032
                     /locus_tag="ACHY5W_004193"
     CDS             2770..4032
                     /locus_tag="ACHY5W_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4448962.1"
                     /translation="MSLNDSKIRKLKSSSRPVKLSDSHGLYLLVNPGGSRIWYLKYRF
                     NGKESRGSLGAYPLVSLAEARQQRDGIRKLLAQNINPAQQRMAEKSACSPEKCFKAVA
                     LAWHKTNKKWSADYAARILASMENHIFPAVGHLPVAALKTQDFTALLRVIEDKGFLEV
                     ASRTRQQLSNIMRYAVQQGLTDSNPAQHLEGVVSSPVKNHYPALPLERLSELLGRIGD
                     YQQGRELTRLAVVLTLHLFIRSSELRFARWSEIDFRHKIWTIPATREAIDKVRFSGRG
                     AKMRTPHIVPLSRQAIALLKQIQELSGHLDLVFPGDHNPYKPMSENTTNRALRLMGYD
                     TKTEICGHGLKAMACSALVESDLWSRDTVERQMSHQERNSVRAAYVHKAEHLEARKAM
                     MQWWSDYLDVCREGYVAPYIYARQHKAA"
     gene            complement(4259..4558)
                     /locus_tag="ACHY5W_004194"
     CDS             complement(4259..4558)
                     /locus_tag="ACHY5W_004194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448963.1"
                     /translation="MSGILWRIFWDVLSGNTVEISIIESFSPSVINMLITEGGCLLKT
                     DVLIGFSPEIIGNKIISDLHLSGLSISDAVLTGFRVYGQEIFWSINWYFRENIPY"
     gene            complement(5331..>5804)
                     /locus_tag="ACHY5W_004195"
     CDS             complement(5331..>5804)
                     /locus_tag="ACHY5W_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS4448964.1"
                     /translation="LEIARLMGLRSQEAVQSSQSLKTWLKTIERGENRLKVVFGTKGG
                     RPRHTTVLDTGAVRKALEKALLAAEQCNSRLIDKPDLKTAMNHWHRQAVKVGLTGEFS
                     PHSLRYAWAQDAIRHYLEQGFSEKESLALTATDLGHGDGRGRWVKQVYGYRWKEE"
BASE COUNT         1579 a         1388 c         1343 g         1494 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaagggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
     1321 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
     1381 aagaaaatat tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag
     1441 tgattgaatt ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg
     1501 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
     1561 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
     1621 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
     1681 cccttccctg gcaggaagca ctatcgatcc cccttttaac gtgtctgacg gaacaggaac
     1741 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
     1801 agggctttga actggattca ttaagaagct gccggatagc acttctattt tgcctacccg
     1861 ttctggagtt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1921 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1981 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     2041 attcttttga tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     2101 cccgtaacgg cgatcgcgca agcggagttc cctttattcc gttgcgtgag gttgctggct
     2161 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg
     2221 agaatgcggc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     2281 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     2341 aacgtttctg ccaattttat caacaagatc ctttgcagag actgcatcac gctaatgata
     2401 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     2461 ttgaaatgac ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgccc
     2521 cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     2581 actggttcga gtccagtcag aggagccaaa ttcctgtttt catgcttatc tgtacgtctc
     2641 tgtttgttta ggattcagta agttagcatg aaaatccttc ccggtgcgta tctggtttta
     2701 tctccgcatc gggataattt ggtggtcaga tctggggtca gtttggttcg attctggagt
     2761 gacccccaaa tgtctttaaa cgattctaaa atccgcaagt taaaatcttc ttcccgtccg
     2821 gtaaaactct ccgactctca cggtctgtat ctgctcgtca atccgggcgg ttcgcgcatc
     2881 tggtatctca aatatcgttt caacggaaaa gaatccaggg gcagtcttgg cgcatatccg
     2941 ctggtctcac tggcagaagc caggcagcag cgcgacggta tccgtaagct actggcgcag
     3001 aatatcaacc cggcgcaaca gcgcatggca gagaaatccg cctgctcccc tgaaaagtgc
     3061 tttaaagcgg tggcgctggc ctggcacaaa accaacaaaa aatggtcggc tgattatgcc
     3121 gcccgtattc tcgccagtat ggaaaaccat atcttcccgg cggttggtca cctgcccgtt
     3181 gctgcgctta aaacgcagga tttcaccgca ctgctgcggg ttattgagga taagggcttt
     3241 ctggaagtcg cgtcccgcac gcgacagcaa ctcagcaaca tcatgcgcta tgccgttcag
     3301 cagggactta ccgacagtaa cccggcgcag catctggaag gcgttgtctc ctcccccgta
     3361 aagaatcact accccgcgtt accgctggag cggttgtccg aactgcttgg ccgcatcggt
     3421 gactatcagc agggccggga gttaacccgg ctggcggtgg tgctgacact gcacctgttc
     3481 atccgttcca gcgaactgcg tttcgcccgc tggagcgaga tcgatttcag gcacaaaatc
     3541 tggaccatcc ccgccacccg cgaggccatc gacaaagttc ggttttcggg gcgtggcgca
     3601 aaaatgcgta ccccgcatat cgtaccgctc tcccgccagg cgattgccct tctgaaacag
     3661 atacaggagc tttccggcca tctggatctg gtgtttcccg gcgatcataa tccgtacaag
     3721 ccaatgagcg aaaacacaac caaccgggcg ctgcgcctga tggggtatga cacgaaaact
     3781 gaaatctgcg ggcatggact caaggcaatg gcctgtagcg ccctggtgga gtcggacctg
     3841 tggtcacgcg atacggtgga gcggcaaatg agccaccagg agcgcaacag cgtgcgggcg
     3901 gcatatgtgc ataaagcaga gcatctggag gccagaaagg ccatgatgca gtggtggtcg
     3961 gattatctgg atgtgtgccg cgaggggtat gttgcgccgt acatttatgc ccggcagcat
     4021 aaggcagcct gaatcagaaa tagcgtaaat cattaaaacc ggaaaagcag atcctgattg
     4081 accgggatct gtttttttgt attgagaggt attgttgctg tttttgttat tattaattgg
     4141 gaagcaccga aaattattcc agtagaatat ttgcaggcga ctgcctatta ataaatgcat
     4201 tattgataga taactacctt ctgtgatttg cccgcaaatc acagaagatg aaaacctatc
     4261 aatacgggat attttcacgg aaataccaat taatactcca gaatatctcc tgaccatata
     4321 ctctgaaacc tgtaagaact gcatcagata ttgagagacc agatagatgt aagtctgaaa
     4381 ttattttgtt tccaatgatt tcaggcgaaa aacctatcaa tacatctgtt ttcaataggc
     4441 agccaccttc cgtgattaac atgttaatca cggaaggtga aaagctttca ataatcgata
     4501 tttccacggt attaccactt aatacatccc aaaaaatgcg ccataaaata ccggacaatc
     4561 tttcctgata cacattatca ctgcaccagc tatacagaga caaaataatc tgcacccgtc
     4621 ctggataatc tgctatctgc gccagcgtcc cgccgctcta cctgttcagg gctgcgccag
     4681 ttcctggccc tttagttttt ggccctttca ggtcaggcgc tgttaccctt atcgccccct
     4741 taacctttat gaccctttcc ctttctggcg caaccagttt atttggaaat gctccatgac
     4801 agtcggcacc agactgtatt ttcaccacag gataacgatc acaaagtgca aaaaataaca
     4861 tgaagcaccg aagattctta attcatatga agcaggttat tatgccaaaa accgtcgcag
     4921 catttttata aaatcatgag gcatcctgag accatataat atatgtcaca ggatacggaa
     4981 aaacacagag agaattattc agatataagc ctgcaccaaa attaatgtga tgccccggtg
     5041 aatttacatt ttaaaatttc cccagccctg tagtattggt aaaaatgaat gaaacacctg
     5101 ttatcaattg gaatcgtttc gagtgattac agggtaatca ctataatatc ccggcaaact
     5161 atattggtta agcaggagaa tctgatctgt tgatatttat tcgtcatact gtaacgatat
     5221 tttcacaata taaacccgtt cgccccctgc gccagatccg gaacataggg tacgataaat
     5281 cgctgtcgtt acacagtaac cgcattattt tgcgccattc atgaactcag tcattcctcc
     5341 ttccagcggt agccgtacac ctgtttcacc cagcgaccgc gaccgtcgcc atgacccaga
     5401 tccgtcgccg tcagcgccag tgactccttt tcgctgaatc cctgttccag ataatggcgg
     5461 atcgcatcct gcgcccatgc ataacgcagg gagtgcgggg aaaattcgcc tgtcagcccc
     5521 actttaaccg cctgtctgtg ccagtgattc atggctgttt taaggtcggg cttgtcgatc
     5581 agcctgctat tgcattgttc tgctgcaagt aacgcttttt caagggcttt cctgactgcg
     5641 ccagtatcca ggactgtcgt atggcgggga cgaccgccct tggtgccaaa caccactttc
     5701 agccggtttt ccccgcgttc aatggttttc agccacgttt tcagcgactg tgaactctgt
     5761 accgcctcct gcgaacgtag ccccatcagc cgcgcaatct ccag
//
LOCUS       DAZUGK010000240         5778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZUGK010000240 DAZUGK010000000
VERSION     DAZUGK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.242"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(99..383)
                     /gene="cas2e"
                     /locus_tag="ACHY5W_004196"
     CDS             complement(99..383)
                     /gene="cas2e"
                     /locus_tag="ACHY5W_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFS4448965.1"
                     /translation="MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPF"
     gene            complement(385..1302)
                     /gene="cas1e"
                     /locus_tag="ACHY5W_004197"
     CDS             complement(385..1302)
                     /gene="cas1e"
                     /locus_tag="ACHY5W_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFS4448966.1"
                     /translation="MAWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGIRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYVPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(1318..1908)
                     /gene="cas6e"
                     /locus_tag="ACHY5W_004198"
     CDS             complement(1318..1908)
                     /gene="cas6e"
                     /locus_tag="ACHY5W_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFS4448967.1"
                     /translation="MYLSKVIIARAWSRDLYQGLWHLFPNRPDAARDFLFHVEKRNTP
                     EGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDS
                     KGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCF
                     EGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(1895..2569)
                     /gene="cas5e"
                     /locus_tag="ACHY5W_004199"
     CDS             complement(1895..2569)
                     /gene="cas5e"
                     /locus_tag="ACHY5W_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFS4448968.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVTGDHLKFTARDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(2596..3663)
                     /gene="cas7e"
                     /locus_tag="ACHY5W_004200"
     CDS             complement(2596..3663)
                     /gene="cas7e"
                     /locus_tag="ACHY5W_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFS4448969.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KRAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKS"
     gene            complement(3676..4158)
                     /gene="casB"
                     /locus_tag="ACHY5W_004201"
                     /gene_synonym="cse2"
     CDS             complement(3676..4158)
                     /gene="casB"
                     /locus_tag="ACHY5W_004201"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFS4448970.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKPEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(4151..5659)
                     /gene="casA"
                     /locus_tag="ACHY5W_004202"
     CDS             complement(4151..5659)
                     /gene="casA"
                     /locus_tag="ACHY5W_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFS4448971.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVRGIDLRSTVLLNVLTIPRLQKQFPNESHTENQPTWIKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT         1571 a         1446 c         1183 g         1578 t
ORIGIN      
        1 gtatacattt gttcttaaag cattttttcc cataaaaaca acccaccaac cttgatgtaa
       61 catttcctta ttattaaaga tcagctaatt ctttgttttt aaaaaggtaa aaaagacacc
      121 aaccttaaac catccaaatc taccggggta cgcctgttta ctccaaatgt ctggaactca
      181 aatcccgatt ctgtattcgt tgcccatgcc atcactacat tgccctcctc cgccagtccg
      241 gatatttgtt cccagatcat ttcacgaatt tttgcggata catcacctac atatactcct
      301 gcacggactt ccagcaacca gatggctaat ctgcctcgta agcgcggagg tacattttca
      361 gtgaccacga ccaacatact catctcagct actccgatgt ccggcatctc ccagagaaac
      421 gggaagtgga atagcaacag gctgcgcatc ttcaggtggg gccggcggct gtatttcccc
      481 agcagcaagc acgtcctcta taagcggaat caatttggct aatgttttac tactgcgaaa
      541 aatatccctg cacgccaaac gtacttcccg gtccggctca ccaggattac gacgcgctat
      601 ctcaaaagct ttcggtacaa cagtgtcaaa tttaatgata tccgcgatat cgtaaacaaa
      661 ggaaagaggc tttcctgtat gcacaaaacc aatagctggt acataaccag ctgcaagtat
      721 cgccgcttca gttacgccgt ataaacagga agttgcagcg ctaatgcatt ggttgaccgt
      781 atcgcccttt tcccagtctt tcggatcgta gcgacgtcca ttccatgtca cgccgtactg
      841 cttcgccaga agtgcataag ttgctcgcac acgacttcct tctatacctc tgagttgttc
      901 tacggagcgt cgggcgggcg caggttcacc aaaccgaagt tcaaacattt tgcgtactac
      961 cttcagacgc aaatcttcat ccagagcaag ttttgcctga tagagtagct tatctgaacg
     1021 cgcaccacca ggctggccag aagcataaac acgaatgccc gcttccccca cccataccaa
     1081 caatgttcca acttgcgcag ccaagcgtac agctgcatgc gaaacccgtg taccaggttc
     1141 cagcatgatg caggcaaccg agccaacagg aatatgagtg cggatccctg tcttgtcgat
     1201 aagtacaaac gcgccatcta ttacatcgat ctgcccatat tgcagaaaga tcatggagac
     1261 gcgatctttg agtggaatgg ggttaagggg aagccaagcc attttattac acctcaatca
     1321 cagtggagcc aaagatagca agccacatcc catcgattta gctggcccaa taccttgctg
     1381 tacaagatct attaacgctg gcgcgtcgtt gatggtgagc acaccttcaa agcaaaccgt
     1441 ttggatcttt ccacttttac catcaccaga aaaatactgt ggccgttccg atatgggatg
     1501 cacatcttca acgcgcgccg cattgcccaa tttacgttgc aaccacgcga tttgttctgc
     1561 ttcttttatt aacggaaccc gacagcgttt aatattccct ttactgtcca ggcgcttttg
     1621 attgtcgaga atagttttga tcggatttgc ccgaagccga aaatagagtg gaacaccaac
     1681 ctgaagttga aattcaacct gtttagtttt aatgactgtc gcaacggcag ttgaaacagg
     1741 catttgcgct gactgcaata aaacatgaca gccttctggt gtgtttcgct tctcaacatg
     1801 aaaaagaaaa tcacgagcag catccggtct gtttggaaat aaatgccata atccctggta
     1861 aagatccctg ctccaggccc tggcaatgat gactttactg agatacatcc atacctcctt
     1921 taatcacata ccattctcgg gaagcaaatt gtcgaggtaa ggtgatcatc ggttcgtcgc
     1981 gcgccgtaaa ttttaaatga tcccctgtaa ctgattcctc actatatata tcgccgccaa
     2041 cgggctcata atttaatagc gccttctgag gatccgatgc ctgacatgtc cccaaaaaaa
     2101 gcggatgtgt tagtgggcaa cttctccgcc ccaggtaggg tgtataccga ggctttaata
     2161 ctgctttttc aagttctgag ataaccatcg ttgcatgggg tgttaaccag agagcgacgg
     2221 taaaggaggc atcacataaa tattcgcgcc atgtttgaat cgtttcatga cttttcaaac
     2281 cacggtaatc ttctcgcgct ccaaggactg tatggtaatc acgcaacccc gttacagaca
     2341 cacgacgatc gtcaagaatg agttcatcgc agcgcactgc aaattgcaca ctctctgata
     2401 acgcctgtaa tgaagaagta tcatcacgtt ggatcccaag acaagccccg agtagcccta
     2461 ataacccgct tcgggtcgga aatcttccgg taggtcgcgt tccttcaaag gtcggctgcc
     2521 cccaggcttg cattggccca gcaagccgca agatcaaata agatctcatg ttcacgcctc
     2581 gccattatta cgaacctagg attttaactg ttctaaagta ggcatttgtt taacttgagc
     2641 agtaattggg tctacatcag ataagctgaa ttgcgcagca gctccgttca gaccatatcc
     2701 attggcaacg cgatcccaat attgattaaa cgcctgtata gacggttgca aaaagccatc
     2761 tttcgcttta accgcttttt caaaagcatt tgccatagaa agtggcatat cggagaaatt
     2821 aaccattacc atatccgcag ggttaaaagc ggcataagta cgctgttttg ctccagggac
     2881 ctctgttgcc agcatatgaa caacatgggt tgcaatttcc agagcctgct ccctggaggc
     2941 accacctaaa ttttcctgaa gttgagcgag gttaatgttg gcataacgat aaaaaacacc
     3001 cgatgaaaat tcctgagttc ccagatgtgc agaaccttgt tcctgtaaat catctacagc
     3061 ggtgaaccag tcaatatcag aatcaacctg atgagtagtg atcgcatgcg caatggacat
     3121 tgcaccatca acttttccca actcactcat catgccgctg gttgccattc ttccactaag
     3181 cgcaatatca acaccctgct gtaaattcac acgtatggcg gcaatatctt ccttaagaac
     3241 tttgagcagc tttttatcat ccagattatc agcctctgct tttgcaacct gctcacagaa
     3301 ccaggctatt tctcccacaa cccagggagt aaccgcatcg gcagaaatct tttcggcttc
     3361 atcaactaat ttaccggaga gcagcgctaa tgtcttatcg attatttttt ggtcaaatcg
     3421 ttcaccaagt ttttgccgaa gaacatcacg taattgtgca agatgaattg ttctgagact
     3481 ggattcacca atattttgtg cgtaataatc acttttacgc atcgcacgtt taaggctttg
     3541 acttgaaatt cttactcgtc ttttcccgcc gaaaatagcg tctttctgca tgttcatatc
     3601 gtcgcggttc agacatgaag ggctgtgaga gatcagaaca tgaatattga taaagttaga
     3661 catagaaagg tttccttacg catttttgtt tgtggtcaat acaaaatctt ccagaagttg
     3721 ctggcgttcg cgctttcccc accaggtcaa catcctggcc attaatggcc agtcaagtac
     3781 gggttcggcg tgagtaagta atcgacgtaa ctggaccata tcggctgttc tgtcagcccg
     3841 aattaattga aagatacggc gctcgttaat tcttccacta ttggctaaag ctcttcccaa
     3901 cgagatacct gttgtttgct ccggtttttt gtcctgatgt cggatgacat tctttcctgc
     3961 gctcaggcaa aacaccatgc gcaaaagagc ctgctggtga cgtgggttct cccaaccaaa
     4021 aggttgcacc agcctataaa acgcagggat atcgcgtaat tcatcaggtt ctgaaacacg
     4081 tctaatttgc gcacatgctc cattatccag ttgttgccag gctcgatata aagccattgc
     4141 atcaatttca tcagccattt gatggccctc cttgcggttt taactcccgt aaatgtttgt
     4201 atagcgtggc gcgggcaagc gctaatgtgc ttattaattt aggatgatgt gcatagggag
     4261 ctacagattg attaaatagc atttcacaca attgatgaag tttgtctcgt aaatcagcta
     4321 tgacctcatc agcctgggaa aaattaacat tcgccagaac atcgggaatt aataattcac
     4381 tctgtcgata gaaatgcctt tctgcagtct catgaacaga gactccggcc cctttgaagt
     4441 ctttattttt aaacccttct gcaaaggtat ataacgcctt gcgtaaggct gttttatatc
     4501 ccaaaccaac agtcactatt tcgtttatca cattgccgta ttgttgccac ccctgattaa
     4561 acatcagcac atcatgacgc cgttcaagaa tagatgcttg attattacga tatcccccca
     4621 taatcaattc aagaggactt tgcggcgcaa tatttctgaa ttgattcaca accgccgcca
     4681 cgcgatttcc attttcattt tgaataatct tatctaccac aactcggctg atttgtgtcc
     4741 atgatggtgc ggaggtggtg aaagcaagaa atttttcctc aacctcccct ttcttgactg
     4801 ttaccagaca aggggaatgc ggatggggcc atagcccatt aactgtaaag gtaaattttt
     4861 ccttaagaaa accggtataa cgcaaattgc tttcctgtcc acagcaagaa catttaccaa
     4921 tcccaatggg atcgcataat tcaatatgcg ctggttgcca gaataagcca cggacaaagc
     4981 caattgacga agctggcaca gattcattgg gcttgacagg cttaatccag gtaggttggt
     5041 tttccgtatg tgattcattg ggaaattgtt tttgaagacg aggtattgtg aggacattga
     5101 gtaacaccgt cgagcgaaga tcgatcccac gtacgaaggt ggttataggt gttcctccac
     5161 gtaaaccgct tttaaagcca ccaccaaaac caggtgcctg attcgcttgg ttgaataacg
     5221 caatcgcagt gcatccacca cataatgctt caccctgacc cggttgattg acaaatgcac
     5281 aattcgtcgc gccgcttacc ccagccaaca gtttttccat tggagtcaca tcatttgctt
     5341 tgacaccttt ggtctgcata aagggatgtt ctgcgtgatt aaggtagaac atatctatcc
     5401 acggcgcgat gagttgttga aactcatctt cagtgagcgg attcattatg cgatgtcgaa
     5461 attcaacgtc atcttttgcc ggggcgataa tttgcccaat gcaaaccagc agtgctaaag
     5521 cggccagttc catatcgtca cggggcaaac ttaatcgcca ctgatctcta ctgcagtata
     5581 gcgattgcag atttatgatt tggactttcc ccccgtttcg cgggcgtaca gggatccagt
     5641 tatcaataag caaattcatt tgttctcctt catatgctcc gacatttctc ctgcatttct
     5701 atacgtcggc acacttccgt tatcggtaaa aacaaagaaa aaatacggcg taattaattc
     5761 aataatcaca ttcactgc
//
LOCUS       DAZUGK010000241         5728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZUGK010000241 DAZUGK010000000
VERSION     DAZUGK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.243"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..754)
                     /gene="dosC"
                     /locus_tag="ACHY5W_004203"
     CDS             complement(<1..754)
                     /gene="dosC"
                     /locus_tag="ACHY5W_004203"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFS4448972.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQ"
     gene            complement(1126..2445)
                     /locus_tag="ACHY5W_004204"
     CDS             complement(1126..2445)
                     /locus_tag="ACHY5W_004204"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFS4448973.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(2576..4111)
                     /gene="gadC"
                     /locus_tag="ACHY5W_004205"
     CDS             complement(2576..4111)
                     /gene="gadC"
                     /locus_tag="ACHY5W_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFS4448974.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSKNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(4267..5667)
                     /locus_tag="ACHY5W_004206"
     CDS             complement(4267..5667)
                     /locus_tag="ACHY5W_004206"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4448975.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQTDTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         1501 a         1406 c         1392 g         1429 t
ORIGIN      
        1 tttgattgaa aataccgtcg aaatcctgaa tcagacgaga gatatggccc acttcagcaa
       61 tgccgctgaa ataatgtcga cctttatggt taaaccacag gccaaaatca gcctggctta
      121 aaggcaaact actgcctaaa tcagaatcca gcaggatttt atagatgata tctatttccc
      181 atgaaagtat tgaggctatt tgccgttctt tttcttcttc ggcgttttcc agtaacgaga
      241 agatacgata gttttcatct tcttttgagg cactactgtc actaaaggta aacgcgcggg
      301 tcatcacttc catagcgata tcaatactgt taatcgagaa atggtagacc tgaagttttt
      361 ctgcggcaga ataatccgaa gagaagatta ccggatagag gatctttttc agcacccgaa
      421 accccatctc gacaatttct accggaattc ctatgcgggc atgcacttcc gcgacggtat
      481 gctggatttg tattagcctt tcgacatcgt caacctgggc agaaagcacg ttaataatcc
      541 agcgttccat cgcacttttc aactgccgct caacttgttc attactcaag aattcttcgg
      601 catgcgggtc gatgcggaca attcgataaa actcgatact cagataatga gcatgcgcaa
      661 cggcaatttc cgcggcttta gcacgaatgg gcggatctgc ctgttcgaca agtccggtcc
      721 actcatcttt cattctttta aaatacatct ccataattca cactcttata aggctgggaa
      781 atcagacgga atcaaaacgc aacgtgcgag atcgactaac tgcaccatat tctcctgaaa
      841 tatgaagata tactgaaaag aaataagcga cttaggacag tttcaatcta cgctactgtt
      901 cttcagaaga gtatagccca tcgtaattat ttttcggtga cagcgaatat cgtatggttt
      961 ttcatattca tacattttta ttaggggatt tatggctgtt taactaagtg tggttaattg
     1021 catggttaat ttgactttag taagcatgat tagtagtggg atagttcaag agggtaacaa
     1081 gccggtgggt aatgtaccgg cttgttacaa agtaagaatg ggagtttaac tgccccaacg
     1141 actttgcaga tagctgaccg cttgttgagt ctgcggttta ttcagatagt cctcacggaa
     1201 caagatggtg ccgctaattt ctggtaccgc atcgttaaga tcgagctgct ttttcagttc
     1261 cggtacgccg ccgttaatca tccagtctgg ctctatcttt gaaggttcac ccactttata
     1321 gaaggcgata ccgatataca ggcgggtcct ggtcggttta acgacatccg cccaccattt
     1381 tgccaacacg tcataacgcg cggcactccg tgagaacggc cagtaaattt ggggagcaat
     1441 gtaatccagc aatccttgtt ccacccatcg acgggtgtca gcgtaggatt cgtcataggc
     1501 tgccgcgcct cgggtatcgg aaccgagcgg atcgtgtgat cggttacgcc acacgcctgc
     1561 cgggctaaca ccaaattcga ctcccggctt aatgctttta atggtgtgcg atacctttgc
     1621 aattaactgc tgagtattgt tgcgccgcca gtctgctttt gacgcaaatg cgcctccgta
     1681 tttacggtac gtttcgttat catttagccg tgaacccggt gactcagtat agaaatagtc
     1741 gtcaaactgc acgccatcta ccggatagcg ggaaaccact tctgcgacta ttgatgtgat
     1801 ccagtcctga acctcaggga tgcccgggtc gaggacaaag cgatcgccag acgttctgat
     1861 ccagtcacgg tgttgcacat agacgctcgc cggttgttga gacagagtgc tattcagttc
     1921 cctgatagta ccgggcttcg tattaaccga tacgcgatag gggttaaacc aggcgtgtac
     1981 tttcatccca cgcttgtggg cttcgtcgag cataaattgc agcggatcgt aacccggatt
     2041 ttcaccaatc ttaccggtca taagatcgga ccatggcaaa atttttgaag gccacagggc
     2101 ggtaccgtcc ggcttgacct ggaaaaagac cgtgtttata ccgagacgtt gcagatgatc
     2161 cagtttgtcg atcatcgcct gttgttgtac acgggcccgg ctggtggggt tactaatgtt
     2221 aaccgaggaa accggtggcc agtcgaggcg agacaccgtg gccagccaga tgccacgcat
     2281 cggttgtgac gattgttgcg tcgtggctgg tggctttgaa cctgctggtg gtgtcaccat
     2341 ggactctgga ggcgtgcttt tacaactaca cagtaaaagt gccagtgcaa ccagtatcgc
     2401 tggtcttcta atcgctaatt tctcgtttcg ggagcagata tccatatgtg ctggtttccg
     2461 gtaaacagat gtgcgctcat tctcgtactt atcccggtta agtcaatacg acagcaagca
     2521 cgaaaaaggg agcgatgaat tatcgctccc ttatcttata accattcaga catagttagt
     2581 gtttcttgtc attcatcaca atatagtgtg gtgaacgtgc acgcgggtgc aggaagaagt
     2641 gaccttttgg tgcgttctta ctgttgattg gctccagagt gacgccggta ttcgctttgc
     2701 ctttacgatc atgaacagca tagagaataa agggcagggc aagtaccacc aggaaactaa
     2761 cgaccagtaa ttcaacatac atatcggtag agtcaccctg gatattatcc ggcggcagga
     2821 aggaaacaat aaacgccata attgaagtca gcagaccgac aatagccacg accagtttca
     2881 cccctttacc accagggata ttaaatgtgc gttttaagtc aggatgttta agaaccaaca
     2941 caatgtagcc aataaacagc atgaaataag cacacagata aatcaccacc gtcagcgcca
     3001 gtgcgatcag gaaggacatg ttgttaccgc caccggtatt ggtgaggatg atcaacgcga
     3061 tagaagtaat caccagctgc gaaatgacca gcgttaccgg tacgccattt ttgttcattt
     3121 tagcgaatgc cgctggcagc aggtttttct gcgctgtgac atacatcccg cgagaaggac
     3181 caacaatcca ggaggcgatt tccgccagaa cacccagcaa cagcagtgcg gagatcacgc
     3241 gaaccgtcca ctcaatttcc ggtgccacat gggacatcag aacggtaaag gtttgcatta
     3301 cccctgcgga gaggttgatt tcattacccg gaatgaccat cgcaatagac aaaccaccaa
     3361 cagagcttaa gcagattgcc gccaccatca gcagtaacat agccaacgga tagtcgcgcc
     3421 ctgggttgct catttcattg acgtgggttg cggatgcttc tacgcccata taactcaaaa
     3481 tgaaggcaac aaatactacc agggtgccca ctttagagaa gtcagggaag aaggtcttcg
     3541 aatccatttc gatagcaacg ggggcaccgg agtgcagata aatagccgct aatgcgatca
     3601 aaataaatgc aggtaacagg ataccggcga agaagccaac tttagcaatt cgcgccgtgt
     3661 atttcgtgcc accaaactgc gttaatgcca gcgcccaaag aatgatgagt gctgcaatag
     3721 ttttggtaat ggggtcttca ttcagcgctg gccatttcag gatgtaggag agtgccccta
     3781 acacgaaata gagcatcgga ataaaaccaa tggcgatttg cagatagcca aatgagatcg
     3841 ctgcaaatcc ccatctcggc cccagagtat ttgataccca ggcgaagaca ccaccttctt
     3901 cccagccgtc gacggtggcc atttccgcag cacaaagtcc cacgggaata aaccataaaa
     3961 tcccgcctaa tagcaggaag aagactaatg aaaagcccga tgttgcgaag gtagggtatt
     4021 cataaacagc cattaccatc gatgccgtta tggcaaagaa tccgagtaat gtgagctgct
     4081 tagctttacc tgtctgtact gatgtagcca tattatcccc ctaaaacggt attcctgtcg
     4141 gaaccgcacc tgttttgttc tgaagcgtat tcagaacaat attttccgtt gctaatgcca
     4201 gtgaacagac tttggaaatt gtcccgaaac gggttcgttt cgggacaccg ttaccgttaa
     4261 acgttatcag gtatgtttaa agctgttctg ttgggcaata ccctgcagtt tcgggtgatc
     4321 gctgagatat ttcagggagg ctttgtagtc ttccagcaac agttcagcaa agtccatttc
     4381 gaagccgcga cgacacataa tgcgcatcac cacgatgtcg gtggcttcac cgccgagagt
     4441 gaaggccgga acctgccagc cgcgcagacg cagacgttca gagaggtcgt acagggtgta
     4501 tcccggatct tcaccatctt tcagtttgaa gcaaaccgcc gggatgcctt cgtccgggcg
     4561 acccgtacag atgaactcat acggccccag tttggcgatt tcatccgcca gataagcggc
     4621 aacctggtaa gaggcgttct gtactttggt atagccttca cgaccaaggc gcaggaattc
     4681 atagtactgt gcaatcacct gacccgccgg gcgggagaag ttgatggcga aagtaccaat
     4741 ctgaccaccg aggtagtcaa cgttgaacac cagttcctgc ggcagcgctt cttcatcacg
     4801 ccagataacc cagccgcagc ccagcggagc cagaccgaat ttatggcctg aagcactgat
     4861 cgatttcaca cgcggcaggc ggaagtccca gacgatatcc ggggcgacga acggtgccag
     4921 gaagccaccg ctggcggcgt cgatgtgcat gtcgatgtcg ataccggtgt cggtctggaa
     4981 tttatccagc gcatcgtgca gcggttgcgg gaactcatag ttaccggtgt aggtcacgcc
     5041 gaaagtcggc accacgccga tggtgttttc gtcgcaggct tcaatcatgc gtttcgggtc
     5101 cataaacaac tgaccggggc gcatagggat ctcacgcagc tccacatccc agtagcgggc
     5161 gaatttatgc cagcagattt gcaccggacc gcacaccagg tttggtttat ccgttggttt
     5221 gcctgcagct tccatacgct tgcgccaacg ccatttcatc gccatcccgc cgagcataca
     5281 ggcctcggaa gaaccaatgg tgttggtgcc aacggcctga ccatttttcg gcgcaggcgc
     5341 atgccacaga tcggcaacca tatttacgca acgcaggtcg atggctgcag attgcggata
     5401 ttcttctttg tcgatccagt ttttgttaat ggataaatcc atcaacttgt ggacattttc
     5461 gtcgtcccag gtctggcaga aagtggccag gttctgacga gcgttgccat caagatataa
     5521 ttcgtcattg ataatctgaa atgcgacatc gtcgcgcatt tcgtgcagcg gaaaacgttt
     5581 tgattctgcg atagtggaaa tagacttcgc accaaaacgt gaatcgagta gttccgacct
     5641 taaatccgtt acttgcttct tatccatttt aaactcctta aaatgattgg atcgcattaa
     5701 aaaagtagga tttatcgata aagtaagc
//
LOCUS       DAZUGK010000242         5674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZUGK010000242 DAZUGK010000000
VERSION     DAZUGK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.244"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..353
                     /locus_tag="ACHY5W_004207"
     CDS             105..353
                     /locus_tag="ACHY5W_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4448976.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            331..1461
                     /locus_tag="ACHY5W_004208"
     CDS             331..1461
                     /locus_tag="ACHY5W_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4448977.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1507..2145)
                     /gene="ompW"
                     /locus_tag="ACHY5W_004209"
     CDS             complement(1507..2145)
                     /gene="ompW"
                     /locus_tag="ACHY5W_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFS4448978.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2385..2474
                     /locus_tag="ACHY5W_004210"
                     /pseudo
     CDS             2385..2474
                     /locus_tag="ACHY5W_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2502..3245
                     /gene="yciC"
                     /locus_tag="ACHY5W_004211"
     CDS             2502..3245
                     /gene="yciC"
                     /locus_tag="ACHY5W_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFS4448979.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3275..3814
                     /gene="yciB"
                     /locus_tag="ACHY5W_004212"
     CDS             3275..3814
                     /gene="yciB"
                     /locus_tag="ACHY5W_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFS4448980.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3919..4317
                     /gene="yciA"
                     /locus_tag="ACHY5W_004213"
     CDS             3919..4317
                     /gene="yciA"
                     /locus_tag="ACHY5W_004213"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFS4448981.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4357..5076)
                     /gene="tonB"
                     /locus_tag="ACHY5W_004214"
     CDS             complement(4357..5076)
                     /gene="tonB"
                     /locus_tag="ACHY5W_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFS4448982.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPQKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPTSPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5300..5596
                     /gene="yciI"
                     /locus_tag="ACHY5W_004215"
     CDS             5300..5596
                     /gene="yciI"
                     /locus_tag="ACHY5W_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFS4448983.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1435 a         1336 c         1377 g         1526 t
ORIGIN      
        1 tatctggcaa aaactgatcc cgaatcaggc tgatttttaa tgttcaaccc cggtcgttgc
       61 ccaccagcta tagtggcggc gaccatgatt agcgaacgac gctcatggca agacttattc
      121 tgctcactga gtgggcaaaa gaggaattca gtgaaccggt cccaactccg agtacgttaa
      181 gtaaatacgc taaagccgga atgatatttc ctctccccaa aaaagttgga agacgctggc
      241 gagtggatcc gcaagctcgc tttgtcggaa tggtaaacaa gccggaggtg atcgccacag
      301 atcaccctgc tttgaagagg atactggaag atggcgcgcc cgcgaaaata taaaaccgat
      361 gttccgggat tatctccgta ttttgacaaa agaaataaca aagtttactg gcgttacagg
      421 catcccataa caggcaaaaa tcacggtctc ggcagtattg accagaaact ggcagaaact
      481 attgcagcag aagcgaacag ccgtcttgcc cggcagcaaa tggaacaaat gctcagtctg
      541 caggagaaaa ttattagtga taccggcggt tcatcaaccg ttaccatttt tctgaataat
      601 tacagaaaaa ttcaacagga aagatatgaa aacggcgaga tcaaactcaa cacgctgaaa
      661 cagaaagcgg cccctctcag ggtatttgat gaacgttttg gcaccagacc gttagatgcc
      721 ataaccgtaa aagatgtggt atcagtactg gaagagtaca aggccagagg acataacaga
      781 atgggacaaa ttttcaggaa agtactgatc gatgttttcc gggaagctca gcaaacgggc
      841 gatgtcccgc caggctttaa ccctgcagaa tcggcaaaaa aaccgcaggt gcggatatca
      901 agacagcgac tgacttttga tgagtggatg atgatttata acgcagcgga aaaggatggt
      961 tactttttac agcgcggtat gctgctggca ctgatgacag gccagcgcct ttcagatatt
     1021 tgcaaaatgc aattttcgga tatccgggat ggttatcttc atgtcgaaca gcaaaaaaca
     1081 ggaacccgga ttgccatccc tctggctctg cgttgcgata aattaaatct caccctggat
     1141 gatgtggtgt catcctgccg cgattgcgtt cttagtccgt ggctattgca ccaccatcac
     1201 gcgaaaggga cagctaagcg cggcgggatg gttaagccag caacattaac cgttgcattt
     1261 aaaaaagccc gggattctgt ggattacaac tggcgtgcta atggcacccc tccctctttc
     1321 catgagcaga gatctttatc agagcgattg ttcagagagc agggggttga taccaaaatt
     1381 ttgctaggcc attcgaatca aaaaatgacc gatatttaca acgacgcacg cggtaaggaa
     1441 tggaaaaaac tggtcatttg atgaccagtt ttgcagaggg gttttgcaga ggttttgcag
     1501 agaaaattaa aaacgatatc ctgcggagaa cataaacacc cacggatcca gtcgtaccga
     1561 gtcttttacg gtagtaacac cagatttgta cttagccgtg gtatcgatat ccatgtacca
     1621 cactgacatg ttaaccaacc agtcacggtt aatcagataa tcaaccccca cctgcccggc
     1681 agctccccag gaatctttca gactgagatc ggaaagcccc gcctctttgc catgatcgtt
     1741 aaatccatta tcaaagaagg tggtgtagtt aatacctgcc ccaacataag gacggaattt
     1801 gctgctggca tcaccaaaat accactgcgc catcagtgtt ggcggcagat gatgaacggt
     1861 tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg ctgccagtaa
     1921 ttccacacca atgttgtcgg tcgccatata agtaaacgtc aggcccagtt gagtgttatt
     1981 ggtcacgctg aatccgccca gacttcctaa cgtaccacca gcaccttctg ttggacgtac
     2041 ggttgcagaa cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg cactgccaga
     2101 gagaagagtt gttactgcca aagccgccac tgttaacttt ttcatatccg ctccgtcgtt
     2161 atggttataa aaacgtgacg aatataccta cattggagta acaaatgatc ctacatagat
     2221 cacattacat atgataattc accctttatt gatctggatt aatttagaag ttgcattgag
     2281 aatcctggtt aacgtgattt aaatcaattt ttaaatattt tcgctacgca aatattaact
     2341 cgttcgccac ttgccgcggt ttgcccacgc ttgaaggcca gcaattacct ccgctttaca
     2401 aagatatgct tttccataca agacctgatg ctgccacccg aggcttaacc agggtacaat
     2461 tgcccgctaa ttaacacctg cataaaactc aaggagagtg catgtctatc acggcgcagt
     2521 ccgtataccg tgacaccgga aatttcttcc gtaatcaatt tatgaccatt ctgttggtat
     2581 cgttgctatg tgcgtttatc acagtggtgt tagggcatgt tttctcaccc agtgatgcac
     2641 agcttgcgca gctcaatgac ggcgtgcccg ttagcggcag tagtgggttg ttcgacctgg
     2701 ttcagaatat gtcaccggaa cagcaacaaa ttttgctgca ggcttcagcg gcgtccactt
     2761 tttcaggatt aatcggtaac gccattctcg ccggaggcgt aatattaatt atccagctgg
     2821 tgtctgcggg tcagagagtc agtgcgctgc gagctattgg tgccagtgcg ccgatattgc
     2881 caaagttatt tattctgatt tttctgacta cccttttagt acagattggc atcatgctgg
     2941 tggtcgttcc gggaattatc atggccattt tactggccct ggcaccggtg atgttggttc
     3001 aggacaaaat gggcattttt gcctcgatgc gtagcagtat gcggctgact tgggcgaata
     3061 tgcgtctggt ggcacccgca gtactgagct ggttgctggc aaaaacactg ttgctgcttt
     3121 ttgcctcttc ttttgccgca ttaaccccgg aaattggtgc cgtactggcg aacaccttga
     3181 gcaacctgat ttcagccgta ttgctcatct atctgttccg tctgtatatg ttgattcgcc
     3241 aataacctta agcatttgat tacggaatcg taaaatgaag cagtttcttg attttttacc
     3301 gctggttgtc tttttcgcgt tttacaagat ttatgacatc tatgcggcta ctgcggcgct
     3361 gatcgtcgcc acggcgattg tgcttatata tagctgggtt cgctttcgta aggttgagaa
     3421 gatggccctg atcacttttg ttctggtggt cgtcttcggt ggcttgacgc tgttcttcca
     3481 caatgatgag tttattaaat ggaaggttac agtcatttat gccctgtttg cgggtgccct
     3541 gttagtcagc caatgggtga tgaaaaagcc gctaattcag cggatgctgg gtaaagaact
     3601 cacgctgccg caaccggtat ggtcgaagct gaatctggcc tgggctgttt tctttatcct
     3661 ttgcggtctg gcaaacatct acatcgcatt ctggctgccg caaaatattt gggtcaactt
     3721 taaagtcttt ggcctgaccg cccttacctt aatcttcaca ttgttaagcg gtatctatat
     3781 ctaccgccac atgccgcagg aagataaatc ctaaccagac tgccagtcct tcggggctgg
     3841 cgctgtatct cctctgttct cctcaggaaa atcatagtag catcgcgcct gtgattttcc
     3901 ttttaagtcg gttttaccat gtctacaaca cataacgtcc ctcagggcga tcttgtttta
     3961 cgtactttag ccatgcccgc cgataccaat gccaatggtg acatctttgg tggttggtta
     4021 atgtcacaaa tggatattgg cggcgctatt ctggcaaaag aaattgccca cggtcgcgta
     4081 gtgactgtgc gggttgaagg aatgactttc ttacggccgg ttgcggtcgg cgatgtggtg
     4141 tgctgctatg cacgctgtgt ccagaaaggg acgacatcgg tcagcattaa tattgaagtg
     4201 tgggtgaaaa aagtagcgtc tgaaccaatc gggcaacgct ataaagcgac agaagcatta
     4261 tttaagtatg tcgcggttga tcctgaagga aaacctcgcg ccttacctgt tgagtaataa
     4321 tcaaaagcct ccggtcggag gcttttgact ttctgcttac tgaatttcgg tggtgccgtt
     4381 aattttaaac aggatattca ccacaatccc actgcctggc ttacccggct cataacgcca
     4441 tctgcgcatc gcatttttca cctcacgctc aaacatgttc gcaggcttgg ctgagaggat
     4501 ttgtacgtta tccacgcgac catccggcgt gacatcaaat ttaactttaa cctgcccttc
     4561 aatgcgcaat gcctgtgctc gtgccggata ctgcggctga ttacggctta atgcgcgtgg
     4621 tcctgaagcc acactggtaa ccggcttgct ggttgcagcc gttgccgtac ttgatgtagg
     4681 gcgcgcgggt gcagtatttt caaacggtga tgtcggacgc gactctacgg gtttgacatc
     4741 gcgttttggc tgctcctgta cctttttcac cggcttcggt tttggcttag gtttcggctt
     4801 cggcttttca atgaccaccg gtgcttcttt ttgcggttcg gggatcggct caggttccgg
     4861 ttctggctct accaccggct ccggtggcgg ctgaacggct tgtggcggtt cgagatcagc
     4921 aggcgcaacc atcgtgacag aaattggctg cgcaggcgca ggtagttcaa taacctgatg
     4981 taccgaggta tagagcagac ccgccacaac agcaccatga atgcagaccg aaagtaacgt
     5041 cggccagggg aagcggcgag gtaaatcaag ggtcattgaa gtcataatca tttcagtaga
     5101 aaaaccaagt ctcgatttta aatgcaaata gcaatcatat tcaataaggc aagacgatca
     5161 ggatcagtga aaatgtcatt tttggcgtaa ttgaccttac tttatgcatg gtcttaacaa
     5221 agacatcatc tttccgttgc agatcttcgc cctttcaaat aacgtacttt acaactttcc
     5281 cgaacaagga gttgtgcccg tgttgtatgt tatttacgct caagacaaag ctgatagcct
     5341 cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt ttacagttac tgcatgatga
     5401 aggtcgtttg ttgacagctg gtccaatgcc agcggtagac agtaacgatc caggggctgc
     5461 gggatttact ggctcaaccg ttattgctga atttgaatcg ctggaagcag ctcaggcctg
     5521 ggccgatgct gacccgtacg tagcggcagg tgtctatgaa cacgtttcag tgaaaccatt
     5581 taagaaagtg ttctgaatag agattcaata tttaagggca cggtttttgc aaccgtgttc
     5641 ttgtttggcc tttccttatt gccggtcagt gcat
//
LOCUS       DAZUGK010000243         5666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZUGK010000243 DAZUGK010000000
VERSION     DAZUGK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.245"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..879
                     /locus_tag="ACHY5W_004216"
     CDS             169..879
                     /locus_tag="ACHY5W_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4448984.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            921..3572
                     /locus_tag="ACHY5W_004217"
     CDS             921..3572
                     /locus_tag="ACHY5W_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4448985.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPNNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSNSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3586..4116
                     /locus_tag="ACHY5W_004218"
     CDS             3586..4116
                     /locus_tag="ACHY5W_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4448986.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWLPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4129..4632
                     /locus_tag="ACHY5W_004219"
     CDS             4129..4632
                     /locus_tag="ACHY5W_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4448987.1"
                     /translation="MKRLHKSFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFSLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4692..5606
                     /locus_tag="ACHY5W_004220"
     CDS             4692..5606
                     /locus_tag="ACHY5W_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4448988.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNS
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATIDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1621 a         1241 c         1260 g         1544 t
ORIGIN      
        1 aaatatttac tttttagcaa ggttttccta ttaaaagaga accaacattt attacaaacg
       61 cattaagtct ggaaacccgg cgactcgcca atgtattagc aacatttacc gattgtctga
      121 tgtaacggtt ttcccgctta tcctcttaac acatggagaa tgcatcagat gcaaactacg
      181 cgtaccccct actctatttc ctttatggcg acagtgttac ttttattgct ctttgcttgc
      241 catagcaccg ttgccaacgc agctgtcgca ctgggtgcga cgcgggtaat ttatccggca
      301 aaccagaaac aggttttatt gcctgtcacg aataatgatc ctgccagtgt ctatctcatt
      361 cagtcatgga tagaaaatgc aggtgaccaa aaagacaccc agtttgtcat taccccaccg
      421 cttttttcca tgcaaggtaa aaaagagaat acgctgcgga taatcaacgc cactaatcat
      481 cagttaccag gcgaccgtga aagcctgttc tgggtgaatg ttaaagctat tcctgctatg
      541 gaaaaagacc agaagaatga gaatacatta cagctggcaa ttattagccg cataaaaatg
      601 ttttatcgcc ccacgaacct ggcaatggca cctgaagaag cgcctgcaat gcttcggttt
      661 cgtcgcagcg gtagcaaact cactcttatt aatccaacgc cctacttcat taccgtaaca
      721 aatatgaaag caggaaatag caatctccca aacaccatgg tgccgccaaa aggtgaagta
      781 tcggtggata tcccgcatgc tgccaccggg gatatcagtt ttcagacgat caatgattac
      841 ggcgcattaa ctccgcgtat taaggcaacc atgcagtaag ccagtgacct aattaataat
      901 aaactcaatc ggaattaata atgacagcct ttcgtgccgc atttaaggca tatcgtatgc
      961 atcaggtgtt actactgcct cgctttgcac ggcttaccat tgcccttagc ttagcaactg
     1021 cggtatttcc cgttgacgca gagtattatt ttaatccacg gtttttaagc aatgatcttg
     1081 ctgaatctgt cgatttatcc gcctttacta agggacgcga ggcaccacct ggtacatatc
     1141 gggtcgacat ttatttgaac gacgaattta tgactagtcg ggatattact tttattgcag
     1201 atgataataa cgcagatctc atcccatgcc tgagtacaga tcttcttgtc agtcttggca
     1261 taaaaaaatc tgcattatta gacaataagg aacattcggc agaaaaacat gtgccgaaca
     1321 acagcgcatg cacaccactt caggatcgtt tggccgacgc atcaactgag tttgatgttg
     1381 gtcagcaaca tttgtcactc tccgttccgc aaatttatgt tggcagaatg gctcgcggct
     1441 atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg gcttctaaac tatagtttta
     1501 atggtaattc tattaataat cgtagtaacc ataatgcagg aaaatccaac tatgcatatt
     1561 tgaatttaca gagtggcatc aacataggta gttggcgact gcgcgataac tcaacgtgga
     1621 gttataacag tgggagtagc aattcatctg acagcaataa atggcagcat atcaatacgt
     1681 cggctgaacg tgacattatt cccttacgct cacggttaac ggtaggtgat agttataccg
     1741 atggcgatat ttttgatagt gtgaactttc gtggcctcaa aataaattca acagaagcga
     1801 tgttgcccga tagccaacat ggtttcgctc cggtgattca tggtattgcc cgtggcaccg
     1861 cacaagtgag tgtaaaacag aacggatacg atgtttatca gactactgtt ccacccggcc
     1921 cttttactat tgatgatatc aactctgcgg ccaatggtgg tgatctgcaa gtaaccataa
     1981 aagaggcaga cggcagtatt cagacattat atgttcctta ttcgtctgtt ccggttctcc
     2041 aacgtgctgg atatacgcgt tatgcgcttg ccatggggga atatcgtagt ggaaataacc
     2101 tgcaaagctc ccccaggttc atacaaggta gcttgatgca tggactggaa ggaaactgga
     2161 caccttatgg cggaatgcaa attgcagaag attatcaggc cttcaacctt ggtattggta
     2221 aagatttagg actttttggt gccttttctt tcgatatcac gcaggccaat acgacacttg
     2281 cagatggtac ccgtcacagc gggcaatcgg ttaaatccgt ctacagcaaa tccttctacc
     2341 agacaggaac caatatccag gtcgcaggat atcgctattc tacgcaaggt ttttataact
     2401 tatccaacag tgcctacagt cgaatgagtg gttacaccgt caagcctcct accggagaca
     2461 gcaatgagca gacacaattt attgattatt ttaatctgtt ctacagtaag cgtggtcagg
     2521 aacaaataag catctctcag cagcttggaa attacggtac gacatttttc agtgccagtc
     2581 gccaaagtta ctggaacacg tcacgcagcg accagcaaat atcatttgga ttaaatgtgc
     2641 cgtttggtga tattacgact tcgctgaatt acagctattc caataatata tggcaaaacg
     2701 atcgggatca tttactcgct tttacgctta atgttccctt cagtcattgg atgcgtacag
     2761 acagtcagtc ggcatttcgt aattcaaacg ccagttacag tatgtcaaac gatttgaaag
     2821 gcggcatgac caatctatcg ggggtttatg gcactctgct gccggataat aacctgaatt
     2881 atagcgttca ggtcggtaac acccacggag gtaatacatc gtctggcacc agtggttaca
     2941 gttctcttaa ttatcgtgga gcttacggta atactaatgt cggttacagt cggagtggtg
     3001 acagcagcca gatttattac ggaatgagtg gtgggattat tgctcatgct gatggcatca
     3061 cctttggaca gccgctgggc gacacaatgg ttctggttaa ggctcctggc gctgataatg
     3121 tcaaaataga gaaccagacc ggaattcata ccgactggcg tggctatgca atattaccat
     3181 ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc gaattccctt gcagataatg
     3241 ttgaactgga tgaaaccgtg gtcactgtca tcccaactca cggtgctatt gccagagcaa
     3301 catttaatgc acaaatcggc gggaaagtat taatgacgtt gaagtacggt aataaaagcg
     3361 ttccattcgg tgcaattgtc actcacggag agaataaaaa tggcagcatt gtcgcggaaa
     3421 atggtcaggt ttatctgact ggacttccac agtcagggaa attacaggtt tcatggggca
     3481 atgataaaaa ctcaaactgt attgtcgatt acaagcttcc tgaagtctct cctggaacct
     3541 tgctgaacca gcagacagca atctgtcgct aaggaacaat agattatgaa atacaataac
     3601 gttattttcc tcggtttatg tctggggtta accacctatt ctgctttatc cgcagatagc
     3661 gttattaaaa ttagcgggcg cgtcctcgat tatggctgca cagtctcatc ggattcgctt
     3721 aattttaccg tagatctcca aaaaaacagt gccagacaat ttccaacgac cggtagcaca
     3781 agtccagccg tcccttttca gattacgtta agtgaatgca gcaaagggac aacgggggtt
     3841 cgggttgcat ttaacggtat tgaggatgca gaaaataata ctctgttgaa actggatgag
     3901 ggaagcaata cggcctccgg tttaggtata gaaatactgg acggaaatat gcgtccggtg
     3961 aaactgaatg accttcatgc cgggatgcag tggctcccac tggtaccaga acagaacaat
     4021 attttgcctt actccgctcg tctgaagtca actcagaagt ccgtcaatcc gggactggtg
     4081 agggcttcgg caacctttac ccttgaattt caataatcag agtcagatat gaaaagactt
     4141 cacaagagtt tcctgttagc tacgttttgc gcgttattca cagcaactct ccaggccgcc
     4201 gatgtcacta tcactgttaa tggtcgggta gtcgctaaac cctgcactat tcaaaccaaa
     4261 gaagctaacg ttaatctcgg ggatctttat acgcgcaatc tgcaacaacc tggttctgca
     4321 tctggctggc acaatattac tttgtcatta accgattgtc cggttgaaac aagtgcagtg
     4381 acggcaatcg tgacaggttc aactgacaat acgggttatt acaaaaatga aggtactgcc
     4441 gaaaatattc agatagagct gagggatgac caggatgcga cgttaaaaaa tggcgatagc
     4501 aaaacggtta ttgttgatga gatcactcgt aatgcacagt tttcacttaa ggcaagagct
     4561 atcacggtga atggaaacgc aagccaggga acgatcgagg cgctaatcaa tgtgatctac
     4621 acctggcaat aacgtcgatt cgattttctt agccttcttt ttaaatcaat tttttaacct
     4681 taggatatat catgggtaaa acaatcagta taaaggtcct gttcggtatt tatcttctat
     4741 taatggcagg caaagttttt gccttcagtt gtaatgtcga tggcgggtca agtattggag
     4801 caggtacaac ctctgtttat gttaatctcg accctgtaat acaaccggga cagaatctgg
     4861 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
     4921 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
     4981 cgctgtactg gaatagtgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
     5041 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagtcgggg
     5101 cggccggtgg agttgtcata aaagccgggg aagttattgc ccgtattcat atgtataaaa
     5161 ttgccacgtt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
     5221 gtgttgttat gccaacaggc ggctgcactg ttgatagtcg taatgtcacc gtgaatctcc
     5281 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
     5341 tctcatttta tctttcgggt gccaccattg acagtgcccg acaagtgttt gctaacacag
     5401 cacctgatgc aacaaaagca tcgggagtgg gtgtctctct gatgcgtaat ggaaaaatac
     5461 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
     5521 taagcgcaac ctatggacaa acagggaaca aagtttcagc cggaaccgtc cagtcagtca
     5581 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
     5641 acgaaaattg cgcggataat tatttt
//
LOCUS       DAZUGK010000244         5641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZUGK010000244 DAZUGK010000000
VERSION     DAZUGK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.246"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            204..464
                     /locus_tag="ACHY5W_004221"
     CDS             204..464
                     /locus_tag="ACHY5W_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFS4448989.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            654..794
                     /locus_tag="ACHY5W_004222"
     CDS             654..794
                     /locus_tag="ACHY5W_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4448990.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(984..1265)
                     /locus_tag="ACHY5W_004223"
     CDS             complement(984..1265)
                     /locus_tag="ACHY5W_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448991.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRTGGYDVVCYESDVTDEMIRQWKQENAE"
     gene            complement(1806..1892)
                     /locus_tag="ACHY5W_004224"
     tRNA            complement(1806..1892)
                     /locus_tag="ACHY5W_004224"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Leu,seq:taa)
     gene            complement(1905..1978)
                     /locus_tag="ACHY5W_004225"
     tRNA            complement(1905..1978)
                     /locus_tag="ACHY5W_004225"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1944..1946),aa:Cys,seq:gca)
     gene            complement(2032..2107)
                     /locus_tag="ACHY5W_004226"
     tRNA            complement(2032..2107)
                     /locus_tag="ACHY5W_004226"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2072..2074),aa:Gly,seq:gcc)
     gene            complement(2259..2807)
                     /gene="pgsA"
                     /locus_tag="ACHY5W_004227"
     CDS             complement(2259..2807)
                     /gene="pgsA"
                     /locus_tag="ACHY5W_004227"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4448992.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2864..4696)
                     /gene="uvrC"
                     /locus_tag="ACHY5W_004228"
     CDS             complement(2864..4696)
                     /gene="uvrC"
                     /locus_tag="ACHY5W_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4448993.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4693..5349)
                     /gene="uvrY"
                     /locus_tag="ACHY5W_004229"
     CDS             complement(4693..5349)
                     /gene="uvrY"
                     /locus_tag="ACHY5W_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFS4448994.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1507 a         1458 c         1296 g         1380 t
ORIGIN      
        1 tctaccccga aatgcatggc acggtaacag gatttaaaag cgagattgat aatcaggact
       61 ggattattgc aaaagccgag cacaccattg ataacagcgg ctttaccacg cagcttgagc
      121 ttgaggcaaa aatcccggaa tggatagcgg aaacagagtg agcaacttag aatagcggca
      181 gcaccacgtt aaaggaggtc gctatgttcc gttgtccgct ttgtggcgca tctgcccgta
      241 tccgcaccag tcgtccggaa aatgattcaa acaccgtgcg gcaaaagtat taccagtgta
      301 acaacctgga atgcggcgta tgcttctcaa cactggaagc tttccataaa ttcacatcga
      361 aacacgcctc cggcgttcac tcatcagaag gtatcccgtg gcatgagctg ccagcttcac
      421 acaggggaaa caatcagatg agtttgcctt tacctcagaa ttaacaggca gaattgccgg
      481 agtaacaaaa aagcgataga ttacgtgcgg gtgcctttcg gctgatggtc ggagggaata
      541 cccgaaggcc ggatgtggaa aggccccgga aaacatttct gtttaaccga ggccctaacc
      601 cgtcaaccta gcaagtgata ggttagcgcc tctccaacaa aggagcaagc gctatgtcgc
      661 aaaaatcgct tacggccatc acgttctgcg tgacggtaat cctcatcatc tggatgctgc
      721 acggttcgct gtgtgaaata cggatgagct tctggggagc ggagtttgcg gcgttcttac
      781 agtgtaagca gtaaggaaac cgcgacgggg aggcaactcc ccgtcaatcg gttgccaggg
      841 taaggccgat aaggcaccct atctcatagg catgaataac aaccccgcag cgtaaaaact
      901 gcggggtttc tttttggaac tctcactagc tagtgagccg ttaactgcac ataatgctcg
      961 cacaacaaat cagttaaaag agattactca gcattctcct gcttccattg ccggatcatt
     1021 tcatcggtaa catcactttc ataacatacc acgtcatacc ctccagtacg gctatatgca
     1081 ctgcgtccac cacatctact accattcctt gcatgattat acggacacgc acaattgcct
     1141 ggataagatt caatggattc tttaattatt tctttcttga tctgagcatc tgactttcct
     1201 gttgctgcat acccactaaa agataccaaa caaaggcaga ttgccattaa taaaccacat
     1261 cgcataccga tacactctat tactaaccaa tcatagccca catcataaaa tcgagatgag
     1321 gagtacaaca cctttaacaa gaaggtgcca tcacgataat atgccgtgca attcatcaaa
     1381 aaaaagatct atatcaaaga gatagagtta ttatcagtgc tgcagacatc agcattcgag
     1441 gcagcagaaa gacagtaaat ctaacgaatt tgtctttttc tcaagatgtt gtcagattaa
     1501 aaaagataga gtagcgcaga caccttttgg tggatggtag gaggaaaaat gaagttgagt
     1561 gggaagatcc cagaaaaaac tttggtttaa ccaaggccct agttcaatat gcgaaatgca
     1621 gatgagttgc aggagagttt tgcttcaccc ttagagttta aacagacgtg tactatcatt
     1681 atttctgcat atcgccacac catcgccact ttaccgccat ttaacaaaat acaaatacaa
     1741 aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt
     1801 tcccatggta cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa
     1861 tcccttgtgt ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg
     1921 agtcgaaccg gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa
     1981 attcttcagg cctttcagcc agacatccgc tgacgccgat gtcttttaaa ctggagcggg
     2041 aaacgagact cgaactcgcg accccgacct tggcaaggtc gggctctacc aactgagcta
     2101 ttcccgcatt catcaagcaa tcagttaatc acttgatttt attatcgtct ggcaatcagt
     2161 gccgccgttc gatgcgttgc attctactta cctggcgcga tgagtcaacg atatttttca
     2221 ccacttttga tcgtttgctg aaaattgcgc cgaaacgatc actgatcaag caaatctgca
     2281 cgcgcagcgc tcaaatattg caacattgac cacagagtca gtaccgcagc cacaaagaaa
     2341 agtgcaatac cggcgtactc aacccaaatg ttcggacgcc acagcagcca tgccaacgcc
     2401 accatctggg cagtggtttt cactttccca atccaggaga cagccacgct actgcgttta
     2461 cccaactccg ccatccattc gcgtagcgca gaaataataa tttcacgggc gatcatcgtt
     2521 gccgccggta aggtcaccca ccagctgtgg taatgctcgg ttaccagcac catggcgata
     2581 gccacgagaa ctttatctgc tacagggtca aggaaagcac caaaccgggt actctggttc
     2641 cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc
     2701 gcggcggcaa acggcgacca ggtgacaggc agataaaaga ccaatacaaa gaatgggata
     2761 aggatgacac ggaacagtgt aagcaacgta gggatattaa attgcataat gacgggtaac
     2821 tatctgttgt cagtaagatt acccctatgt tgctacagag acatcaatgt ttcaacgacc
     2881 agaagatctt ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc
     2941 tggcgttacg taaaccttgc aaaccaccca tatatttcaa caacatttgc cggcgttttg
     3001 gcccgacgcc ttcaatggtt tccagggaac tggtattttt gaccttcgcc cgttttttac
     3061 ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat atgctggata acatgcagcg
     3121 cgggagaatc tggcggcaaa ctgaatccct caccttccgg ctcaaagaac agcgtttcca
     3181 gcccagcctt acgatctgct cctttggcaa cgccaagtag cagcggatga tttttatccc
     3241 atgagacatc cagttcggcg aagacatttt tcgcctgcgc aagctggcct ttgccgccgt
     3301 cgataagtat cacatccggg atcttactgt cgtcgatggc tttaccataa cgccgacgca
     3361 gcacctgatt catcgccgca taatcatcgc ccggcgtgat gccagtaatg ttatagcgcc
     3421 gatactccgc acgcagcggg ccgttagcat caaacaccac acaggaagcg acggtttgtt
     3481 cacccatggt atggctgatg tcaaagcact ccatccgctt cacttccggc aatttcaaca
     3541 cgctggcaag cgcggtcagt cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta
     3601 aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg cgccctatcg ccgcgaggtt
     3661 tggtttgaac attaatcttg cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt
     3721 tatcgctaag attaaaatcg agcaggatct cacccggtaa ggtgcgcatc tggctgcctt
     3781 gtaaatagaa ctgtcctacg aaggtttcca ccacctcgct cagttccgta ccgccaggca
     3841 ctttcgggaa atagctgcgg ctgccgagca ctttgccctg acgaatgaac aatacgtgga
     3901 cacaagccat gcccgcatcg aacgccacac caataacgtc aaggtcgtcg ccggtattgg
     3961 acacaaactg tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga atacgtgccg
     4021 cttcttcaaa ctccagattc tggctggcag tttccatacg gctaatgagt tgcgtaagca
     4081 cctgatcatc tttgccagac aaaaacaggc gcacatactc gacctgctga gcgtattctt
     4141 cttcactcac cagtccttca acgcacggtc ccagacagcg ccctatctga tattgcagac
     4201 acggacgcga gcgattgcga taaacactat tttcgcactg gcgaatgggg aatatctttt
     4261 gcagtagcgc cagtgtttca cgtacggcat agccattcgg gaacgggcca aaatattcac
     4321 ctttggcatg cttcgcacca cgatgcatcg ccagacgcgg gtgggtatcg ccactcagga
     4381 agataaaggg ataagattta tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt
     4441 tgatgtagtt gtgttccagc aacagcgctt cggtttctgt atgagtaacc gttacatcaa
     4501 tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat
     4561 agctggaaag ccgttttttc aggtctttcg ctttgccgac atagataacc gtaccaccag
     4621 catcgtacat gcgataaacg cctggctggc tggttacggt ttttaaaaac gcttttgcgt
     4681 caaactgatc actcactgac ttgataatgt ctccgcatta cacagaccat ggcgaattgc
     4741 caggtgagtc agctcaacat cgccatgaat gtttagttta ctgaacatac gatagcggta
     4801 gctgttcacc gttttcggac tgagattgag ctgttctgag atctcattga ccttctggcc
     4861 cttggtgatc atcagcataa tctgcaattc acgttcagac aaactggcaa atgggctttc
     4921 tgttttttct ggttcgatct ggcttaacgc catttgttga gcgatgtcag aagcaatgta
     4981 acgctgccct gaatagacag aacggatcgc actcacgact tcctgcggag ccgcgccttt
     5041 gctgaggtag cccgcagcac cggcctgcat gactttcgct ggtaaagggt tttctgtatg
     5101 gacggtaagc atgatgattt tgacatcagc tgtggaacgc gcgattttac gcgtcgcctc
     5161 aagaccgcca atgcccggca tactcatgtc cattagcacc acgtcaacgg catttgcccg
     5221 gcaccactta acggcgtctt caccgcacga tgcctcaccg acgactttta taccctttat
     5281 atcttccaga atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa caagtagaac
     5341 gttgatcaaa ggaatatctc cagaaatagg gataacgcta ctgatagtta gtcattcgta
     5401 tattagcggt ttttattgca actttgaaac gttaaaaatg gtggttattc gatttttctc
     5461 tcgtttttgt aaattcgatt gtccacagtt atggtcacgc ccgtcaccaa cggcttccgg
     5521 gatcgtttca cagtaagccc tgcgatgaaa tgtgacaaaa attgacttta ttcagcaaaa
     5581 atgaaaatca gcccgctgaa tatgtaacat taattaaccc gaacaatacg ggcaaaaaac
     5641 a
//
LOCUS       DAZUGK010000245         5606 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZUGK010000245 DAZUGK010000000
VERSION     DAZUGK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.247"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHY5W_004230"
     CDS             complement(<1..140)
                     /locus_tag="ACHY5W_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4448995.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQL"
     gene            complement(382..912)
                     /locus_tag="ACHY5W_004231"
     CDS             complement(382..912)
                     /locus_tag="ACHY5W_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4448996.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(921..1280)
                     /locus_tag="ACHY5W_004232"
     CDS             complement(921..1280)
                     /locus_tag="ACHY5W_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4448997.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1293..2339)
                     /locus_tag="ACHY5W_004233"
     CDS             complement(1293..2339)
                     /locus_tag="ACHY5W_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4448998.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(2341..2613)
                     /locus_tag="ACHY5W_004234"
     CDS             complement(2341..2613)
                     /locus_tag="ACHY5W_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4448999.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2749..3006
                     /locus_tag="ACHY5W_004235"
     CDS             2749..3006
                     /locus_tag="ACHY5W_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS4449000.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3012..3311
                     /locus_tag="ACHY5W_004236"
     CDS             3012..3311
                     /locus_tag="ACHY5W_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4449001.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(3516..3860)
                     /locus_tag="ACHY5W_004237"
     CDS             complement(3516..3860)
                     /locus_tag="ACHY5W_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449002.1"
                     /translation="MTTITREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALASLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(4194..4372)
                     /locus_tag="ACHY5W_004238"
                     /pseudo
     CDS             complement(4194..4372)
                     /locus_tag="ACHY5W_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4374..5321)
                     /locus_tag="ACHY5W_004239"
     CDS             complement(4374..5321)
                     /locus_tag="ACHY5W_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4449003.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(5318..5494)
                     /locus_tag="ACHY5W_004240"
     CDS             complement(5318..5494)
                     /locus_tag="ACHY5W_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449004.1"
                     /translation="MADFGSTKYSVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTAVEAYCDEWGDE"
BASE COUNT         1337 a         1516 c         1193 g         1560 t
ORIGIN      
        1 agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg cgcaacctcc
       61 ccgactgtcg ccggttcggt aacgcttaat tcattaaaca ccactctggc ggtttcggtc
      121 atatcctgct gtttcagcat gtctttttcc ctttttcggt taacgtgaca caccaataac
      181 tcttgtcgaa aaagccagca agctgaaaga acggtattaa taaccaccag cgaatttatt
      241 gcgctgctgt atattacgga cacaaaaaaa ccaccttccg gtggcttcct tgtgcgaaaa
      301 aacttgcatt tcgcctcgcg atacagcttt gcgaagctta caggaattca agctgtttct
      361 gcgtaaaaaa gcaagctttt tttatcgaaa tgaatcgtgc ataggtacat aaagcatgtg
      421 ttcagccacg gctaaccaac ctgcaatacg tttctcacat gtgctgaaac accattccgg
      481 gtgagtacga tttaaacatt ctgccatttt tctcttactc attccccgtc cttcgtacct
      541 ttgccggaga atattgatta gcccgggata ttccccaagc acctcactga taacgcgatc
      601 aataatcaac gcctctgtgt ctgtacaatg tgacaaccag ctcttctgct tccctctggt
      661 catatcccga aaaaatgcct caagttccgg tttttccagc ccggatttct tcatgctgcg
      721 taaaacctca ttaactgctg ttttcgtcag ctttttcgaa accagtaacc ggttaaacat
      781 atttccggat ttacccccac cgatatacga ccaccgcccc cacatccgta atttcccctg
      841 gatccagact gcttccagcg tgttcaggcg taaatgttcg ccgcttttgc ctgtaatttc
      901 cggatatatc atatttacgc tcactcactc tcaattttgt aaatcttcac acccagccgt
      961 ccaccagata ctggctgacc gcgcacaata ttgatttcat caaactgctc gtcatcaatg
     1021 agcacttccg catgcgtcag cgcatccagc ggtgctttca gaatgttgtc caggtcacgg
     1081 cggcgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga caggcttaat
     1141 ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt tccctcctcc
     1201 gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt
     1261 aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg
     1321 tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc gaacgccagc
     1381 ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga
     1441 gattttgtcc ccattccccc ctgaccatga ccaatcaggt gatgcggatc gtcagcaggc
     1501 ttaccacaac acgcacacgg ctgtgtcttc acccagtgcg tgtatttctc gttaacccag
     1561 cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac ggcaatgctg
     1621 accaccgtct tttcctgtgg cggtttttgc tggtgagcgt gaggcagcgg cgcaagattt
     1681 tttgtgcgct gcttcagtat gctggtggcg gtctgctctc ccggtacgat gtcgctttca
     1741 cggtacattg agcggatttt ttccgcacgc aaccccagcg aacgacgtaa taccgcttcc
     1801 ggtagcgcgt ccgccacctg attgcggacc gcccaccagg ataattcagc cagagataat
     1861 tcccgttcct gtgcaccact tattgcgtga cgtatgacgt caatcatcca ggctgtcaga
     1921 ttttgttgag caagctgccc gagtgattcg gatgtctggt cgcgcagctg gttgtcgcag
     1981 tgccagcaca acaccattgc gccggtacca taacggtgaa tgacggtttc gctgtgatga
     2041 taatcgccgt gtggccactg gcaggattta atatggcgca acagccagtc agacaatgca
     2101 ccagcaccac cagcagcacg aatcacccgt gcgttactga aaaacggcag caatgttttg
     2161 tcttccacta gcggctggcg aacggcagga acaaccccgg acggcagatt acgcatgctt
     2221 ttcggttccg gctccaccag tacccgggta ttgtggaata ccggcatgga ttcacgaccc
     2281 ggcttaacga tcaccagccc gagttccggt accagaacag gtcgaagtaa tacccgcacg
     2341 ttacctccag atgcgttgct ggaatgtgcg ggacggacgc ggcgggcgtt cggaataaag
     2401 gagcctgacg tagattatcc agtgacggta gtcgaggcta agggctttct taaactcata
     2461 cccacgtctg cggtagttat gaatcagcca ttcggcctgt tcttcagtgc agggatcgtg
     2521 ctgataccag tcatatttga atgtgtgaga acaccgccct tgcctgctgg caggggcgat
     2581 atcagaattg tgatgtttgg tattgtgcgc catcggtttt ctctgctggc gcagcaggtg
     2641 ccagttgttc aagctggcgt gcggcaatat tgtctctgat ttctgttgtc gtcaacaggc
     2701 aacgtgctat catcgaatgg tgttctatcc tactccgtga ggtttaccat gcgtacaacc
     2761 caacaattca gcattacatt aactaacgaa atggctgaca tggtgcgcgc ccgtgtggct
     2821 tccggtgcct atgcttcaga aagcgaggtc attcgtgaag ggcttcgcgc actgaatgag
     2881 cgcgataaag caatcgaagc gtggttaacg cattcagccg ccccctctct tgattctatc
     2941 cgcgaaaatc caaacaacgg acgctccatt tcacaggttc gcgccgcgat tcgatccggg
     3001 aagtaatctg catgacatat gaagtcatca ttactcctga ggccgaacaa caaataatca
     3061 acctgcacag gtatataacg gagaaagcag ggaacgtcat tgctgacaat tatgccaatg
     3121 cgcttcttga ttatcttgat gggttttcta cattcccgca tcggggcaat aaacgcgatg
     3181 atattcgcca ggggatgcgg gtaactcatt tccgccacag aacgattatt gcttttgccg
     3241 ttgatagcag aaaagtcttt attgtcggta tctatcatgg tgggcaaagt tatgaaaccg
     3301 atttcttata aacttttacc cacatcattc cggtgttaga attaaccgtc cgccccctct
     3361 cttactggcg gattcgtagg ctatataaat caaagatccc ggctcatgtt tatgtcggga
     3421 tcttttttcg gcgatttatc cccagcggca aatcgaatac accaccagcg ccaccgccat
     3481 cgcaattcct accgttgtga atgcttcagg ccaggtcatc gtaaaatatc ctccacgctt
     3541 atcagtccgt tccgctccag ataactcatc gccttatccg gtaatttgca gtctggcttc
     3601 gctttcctca gttgccaggt taactgcttt accagcatgg ttaactcatc gaccagacgc
     3661 tgatatccca ctggtttgta ttcatgcaat ttaccggctg gctctgctgc cagcgatgcc
     3721 agtgcgattt ccagaacagc aatatccatc ttatatgtgc ggatgatgtc atggtcgatt
     3781 gtgcccggta tacacagtct ctgtgcttca atagtctcct ctgcgtgagc tattaactgc
     3841 tctctggtaa tagtggtcat gccgcgtttc cttctttctt attaacaatt acaccgtcat
     3901 atatttcatt aaggtgccct ctcaactcca tgcgccttaa tgcagacaac atgtaatcgc
     3961 attcaacctg cttatttcca gtaaatggct tatcgtcagg attaccccaa cagcaattac
     4021 ccttgggcca cccatgtact ttccgtactc ttccgttaac aacgtgaagt aatccccagc
     4081 caggtggtaa atcctcaatt gaaataattc ccggctcact aataaagaat cgccagtcgc
     4141 ccattccaag agacggattt ttacgaaaac gcttttttct atctgccaac aagtcagcac
     4201 gagaacattt cgcctctatc aggcatgatg ctgaatttct gaatcccata gcatctggct
     4261 gttctccggt actggttaca gctataaagc ggtcatgaaa acaaaccttg aacccgttgc
     4321 gcttaaggaa cttgtacgca atctgacaga gttcgcggtg tgttaacgcc atatcactct
     4381 cctttgatgc gaatgccagc aagccagttt cttatgccga tatattcagc gttcctgaaa
     4441 ccgcttttta catatataaa tggcaagcga agattgtgac cattgactgc caggtagtct
     4501 ttacaaccct gttcggtgaa acagcaggta acgaattcat caatatcttt cacagcaacg
     4561 cgccgccatt tttctggtgg ctctcgaaag ttttcatgaa gtagctcgag acgacgacta
     4621 tggcgtttat tggcttcatt gccatcttcg tcaacccaga caatccggtc atggtcataa
     4681 tcagcatcaa caacgatttc gcgcttttga tacacacaaa acatgggatc tgacgttatt
     4741 cgattgtcct gtgttcgaat attttcaccg atgatgccaa acgaatctgg tgcagatttt
     4801 gtctgcatct cttcgatacg ttcagccatc gcagcacact cttcaaagtt gcttaatgct
     4861 tttcgctccc attcggcgca ttgtttttcc agttctgcta tgcgcttact tccatccgcg
     4921 attactccct cgtaatattc acgctgctcg ttgagttttg attttgctgc ttcaagctca
     4981 acgcgcagct tccctaccgt tagcgcaata tcctcgttct cctggtcgcg gcgtttgatg
     5041 tattgctggt ttctttcccg ttcatccaga agcgccagcg caacatttgg attaaaggca
     5101 gcaataaatt cagcgtttgc gtaagcctga gcatctgttt caaccaggca gttaacatga
     5161 cattctgcaa tcacgccacc gggttctcct ttccattttt gacaaacaaa aactcctgtt
     5221 atattcccat gctgattgcc cgatgtatgc cctacgatgt agcatccttt agttgctttc
     5281 tctgctgctt cacgcagcgc ctgatagtca atctcgctca ttcatcgccc cactcatcac
     5341 aatatgcttc gaccgcagtt ttccctgctt cataatcatc acgccatgct tcagcatcag
     5401 cagcactgcc accacgtaac tctgcatagt ccattaacag ttcatgccat tcttcaaaac
     5461 tgacgctgta tttagttgaa ccaaaatcag ccattttatt cctcctcttc gtcttttatt
     5521 tcgtgatatg agtaattgca gtagttaaag aaaatatctt ttgcttcgtc atgtatttca
     5581 tcaggcgtcg catcatcatc cacttc
//
LOCUS       DAZUGK010000246         5595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZUGK010000246 DAZUGK010000000
VERSION     DAZUGK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.248"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..571
                     /gene="yfcV"
                     /locus_tag="ACHY5W_004241"
     CDS             8..571
                     /gene="yfcV"
                     /locus_tag="ACHY5W_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449005.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            653..3292
                     /locus_tag="ACHY5W_004242"
     CDS             653..3292
                     /locus_tag="ACHY5W_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS4449006.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRRDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPMIQEQTQQVMPTEAPVSVSAN"
     gene            3315..4067
                     /locus_tag="ACHY5W_004243"
     CDS             3315..4067
                     /locus_tag="ACHY5W_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4449007.1"
                     /translation="MTNLFCSAKKSTMLLALLLTTASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKE
                     TLTRSGQEFTVNNPTPYYVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            4083..4586
                     /locus_tag="ACHY5W_004244"
     CDS             4083..4586
                     /locus_tag="ACHY5W_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449008.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            4583..5059
                     /locus_tag="ACHY5W_004245"
     CDS             4583..5059
                     /locus_tag="ACHY5W_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449009.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            5062..5580
                     /locus_tag="ACHY5W_004246"
     CDS             5062..5580
                     /locus_tag="ACHY5W_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449010.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGVSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
BASE COUNT         1501 a         1344 c         1462 g         1288 t
ORIGIN      
        1 acaaataatg agtaagtttg taaaaacagc tattgctgca acaatggtaa tgggtgcgtt
       61 cgcttctact tcaacaatcg ccgctggcaa caatggtaca gcacgtttct acggcaccat
      121 tgaagattcc ccgtgctcca tcgttcctga tgatcacaaa ctggaagttg atatggggga
      181 cattggttca gggatcctga aaaacaacgg gacttctaca ccgaaagctt tccagatcca
      241 tctgcaagac tgtgtgtttg acacccagac aacgatgacc actaccttca ccggtaacgc
      301 gtcttctacc aacagcggca attactacac catttataat accgatactg gtgcggcatt
      361 taacaatgtc agcctggcca ttggtgacgc tcagggaacc tcttataaaa gcggcgcggg
      421 tatcgaacag aaaatcgtaa acgatacggc gaccaacaaa ggcaaagcga agcagacgct
      481 ggactttaaa gcctggctgg tgggcgctgc tgatgcgcca gatttaggta attttgaagc
      541 caacacgacc ttccagatta cttatctcta agtgataccc ccatagcttt cgggctatgg
      601 ggtttttaaa tggaattcgc gcaaattata ataattacgg atggatacgt gtatgcctga
      661 ccattctctt ttccgactgc gggtacttcc ctgctgcgtt gcattggcaa tgtccgggag
      721 ttatgtcaat gcctgggctg aagatgaaat tcagtttgat tcccgttttc tggagttaaa
      781 aggcgacaca aaaatcgatc tgaagcgatt ttccagccag ggttatgtcg agcctgggaa
      841 atacaattta caggttcaac taaataaaca gccgctgacg gaagaatacg atatttactg
      901 gtacgcctca gagaacgatg ccagtaaaac ctatgcctgc ctgacgcctg aactggtcgc
      961 gcagtttggc ttaaaagagg atgtggcaaa aaacctgcaa tggatccacg acggcaaatg
     1021 cctgaaaccc ggtcaactgg aaggcattga tattaaagct gacctgagtc agtcagcgtt
     1081 agtcatttca ttaccccagg cttaccttga atataccgac atcaactggg atccgccttc
     1141 acgcagggat gacggtatat ctggtttaat tgctgactac agtattaccg cccagacacg
     1201 acatgaagaa aatggcgggg atgacagcaa tgaaattagc ggtaacggga cggttggggt
     1261 gaacctcggc gcatggcgtc ttcgtgccga ctggcagact gattatttgc atagtaaaag
     1321 caatgatgac gatgttatca acggtgatga cacgcaaaaa aactgggagt ggagccgcta
     1381 ctacgcctgg cgagccttac cgtcgctaaa agccaaactt ggccttggcg aagactacct
     1441 gaattctgat attttcgacg gctttaacta cgtgggtggc agtatcagca ccgacgatca
     1501 aatgttgccg ccgaatctgc gcggctatgc gccggatatc tccggcgtgg cgcacaccac
     1561 cgcgaaagtg accgtcagcc agttgggccg cgtcatctac gaaacccagg tcccggcggg
     1621 gccgttccgc atccaggatc ttggcgattc ggtctccggt acgctgcata tccgcattga
     1681 agaacagaac ggtcaggtgc aggaatatga catcaacacc gcctcgatgc cgttcctgac
     1741 tcgccccggc caggtgcgct ataaactgat gatgggccgc ccgcaggagt gggggcacca
     1801 cgtggaaggc ggtttcttct ccggcggcga agcttcctgg gggattgcca acggctggtc
     1861 gctatacggc ggggcgctgg cagatgaaca ctatcagtcg gcggcgcttg gcgtcggtcg
     1921 cgacctgtct gtgtttggtg cggtggcctt tgatatcacc cactcgcata cccgtctgga
     1981 taaagagacc gcctacggga aaggttcact ggacggcaac tcgtttcgcc tgagctattc
     2041 caaagacttc gatgaactga acagccgcgt cacttttgcc ggataccgct tctcggaaga
     2101 gaacttcatg accatgagcg agtatctcga tgccagcgac agcgaaatgg tgcgcactgg
     2161 caacgacaaa gagatgtaca ccgccaccta caaccagaac ttcagggatg ccggtgtgtc
     2221 tgtttatcta aactacaccc gccataccta ctgggatcgc gacgaacaga ccaactacaa
     2281 cgtcatgctc tcgcactact tcaacctggg cagtatccgc aatatgagca tttccatgac
     2341 cggataccgc tacgagtatg ataaccaggc cgataaaggt gtgtacatat cgctcagcat
     2401 gccgtggggt gacagcagca ccatcagcta taacggcaac tacggcagcg gttcggacag
     2461 cagccaggtg gggtatttca gccgtgtcga tgacgcaact cactaccagc tgaacgtggg
     2521 gaccagcgac aatcactcca gtgttgacgg ctattacagc cacgatggct cgctggcgca
     2581 ggtggacctc agcgccaact atcatgaagg acagtacacc tcggcaggta tttccttaca
     2641 gggcggcgcg acgctgaccg cgcagggtgg cgcgctccac cgtacccaga atatgggcgg
     2701 tacgcgcctg ctgattgatg ccgatggcgt ggccggtgtt ccggtggaag gaaacggtgc
     2761 ggcggtttac accaatatgt tcggtaaggc cgtggtggcc gacgtcaaca actactaccg
     2821 caaccaggcg tatatcgacc ttaacaacct gccggaaaac gccgaagcca cccagtccgt
     2881 ggtgcaggcg acgcttaccg aaggagccat tggctaccgt aagttttcgg tgatcagcgg
     2941 gcaaaaagcg atggcggtgc tgcgtctgca agatggcagt tatccgccgt ttggcgcgga
     3001 agtgaaaaac gacagcgcgc agaacgtcgg tctggttgac gatgacggca acgtctacct
     3061 cgcgggcgtc aaacctggcg agcatatgat cgtttcatgg ggcggtgtgg cccactgcga
     3121 tattcatctg cctgacccgc tgccagccga tctgttcaat ggcctgttat taccatgcca
     3181 gcaaacaggg gcgatatctc cttcgatgcc tcatgaaatt aagccgatga tccaggagca
     3241 gacccagcag gtgatgccaa cggaagcacc ggtatcggta tccgccaatt aataacgtga
     3301 ttaaggaatg atccatgacg aatctatttt gctctgccaa aaaatccaca atgttgctag
     3361 cattgctact aaccactgcc agcctgtcag cacaggcctc tgtcacgcca gaccgtacac
     3421 gcctgatttt taatgagagt gataaatcaa tcagcgtcac gctgcgtaat aacgacccga
     3481 aaatgccgta tctggcacaa agctggatgg aagatgaaca gggtaacagg attagctcgc
     3541 cactgacggt cctgccgccg gtgcagcgca tcgattccat gatgaacggc caggtgaagg
     3601 tgcaggggat gccggatatc aacaagctgc ctgccgaccg cgagagcgtg ttctacttca
     3661 acgtgcgtga aattccgccg aaatcgaaca aacccaacac gttacagata gcgctgcaaa
     3721 cgcgcatcaa gctgttctgg cgaccaaagg cgctggaaaa cgtcagcatg aaaaacccgt
     3781 ggcagcataa ggagacctta acccgcagcg gtcaggagtt taccgtcaat aatcctacgc
     3841 cgtactacgt ggttatcagt aacgccagcg cgcagaaaaa tggcaatccg gcagcgggat
     3901 tttcccccat ggtgatatca ccaaaaacct ccgtgccgtt aaaggtcaaa atggatagcg
     3961 tcccggtgct gtcttacgtc aacgactatg gcgcacggat gccgctgtat tttaaatgtg
     4021 gcggtgatac ctgccaggtg gatgtagagc agagccgcaa aggctgatgc cgggagagcg
     4081 acatgagaca aatttttgtt gtgggggtgg gtatattact gctgggcgtt agtgggatat
     4141 ataacattat ccttgcaaat tcaaaacctg ttgatctcat tttacaggta catgtagatc
     4201 ctcctccgcc atgtagcgta acggggagtt cagtagattt tcagactgtg aatatcaacg
     4261 atatcaatgg tagtaattat cgtaaagatg caggatatac gttgtcatgc cctgaccgaa
     4321 aagctgatga tttacgtatg cagttgagag gaaacaccac cacaattaat ggagaaaaag
     4381 tcgttgatgt aggggttcca ggatttggta ttcgaatcga gaatgcagat aacaactcac
     4441 tattctccat tggagaaaac aactggaccc ctttcaatat cacatcacag ccgaaactta
     4501 atgcagtccc ggtaaaacaa agtggtgcac aactttcagg ggccgatttt agtgggagtt
     4561 taacaatggt ggtggattat caatgaaaat actcttgtat ttattgctct gtatgagcag
     4621 cggtattgtg aatgcatcac cggatatcac cttcaaagga actcttgtat tgcctccggc
     4681 atgtactatt agtgatggaa acactatcga ggtggagttc agggatgtaa ttatcgatag
     4741 cattgatgga aataatgggc gagaggtcgt gccctacgat attaaatgtg atgctgttac
     4801 acccggtagt tcctgggata tgacattgac atggataggc acacaaacaa gttatgatga
     4861 tgctgcaatc gaaactgatg taacaggatt agggattaaa cttcggcaaa atggtcagcc
     4921 atttagagta aatacaccga tacaaattaa cgccgatacg aaaccacaat tagaggctgt
     4981 ccccgtgaag gcggtagatg ctgtattaac agacggaacg ttttcagctt cggccacatt
     5041 acgggtggaa tatcaatgaa gatgaaaacc tggttcgcac tcatagtaat gctgttactg
     5101 gcaggaaata gcacagcgcg tgcagacaat aatttgcact tctacggaaa tttattaagt
     5161 aaatcttgta ccctggtagt acaaggacaa gtgctggctg aagtccattt cccaagtatc
     5221 agcagcagag atttaatgtc acgggaacaa tctgatcgtg tgccggtcgt ttttcaattg
     5281 aaagattgca aagggcctgt tggttacaaa gttagagtta cgttaaccgg taccgaagat
     5341 agcgatttgc cagggtttct ggctttggat gcatcatcgg gtgcgcaagg tgtgagcatt
     5401 ggcatggaaa cagttgatgg gatacaggtc cccatcaaca attcggcagg ggcaatattt
     5461 aacctgagta acggtgctaa taatcttaac tttaaggctt ggttgcaggc gaaatctggg
     5521 cgtgaagtca cttctggtga atttactgcc agcctgacgg caatttttga gtatatctaa
     5581 atgaaaataa aagtg
//
LOCUS       DAZUGK010000247         5584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZUGK010000247 DAZUGK010000000
VERSION     DAZUGK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.249"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..160
                     /locus_tag="ACHY5W_004247"
     CDS             <1..160
                     /locus_tag="ACHY5W_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4449011.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /gene="yfgO"
                     /locus_tag="ACHY5W_004248"
     CDS             complement(198..1259)
                     /gene="yfgO"
                     /locus_tag="ACHY5W_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4449012.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="ACHY5W_004249"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="ACHY5W_004249"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFS4449013.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="ACHY5W_004250"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="ACHY5W_004250"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFS4449014.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKENLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4154)
                     /gene="hda"
                     /locus_tag="ACHY5W_004251"
     CDS             complement(3453..4154)
                     /gene="hda"
                     /locus_tag="ACHY5W_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFS4449015.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLALPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHY5W_004252"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHY5W_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFS4449016.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLYRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
BASE COUNT         1523 a         1513 c         1407 g         1141 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttcctgca ctgacggtca gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatggcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt gccgtcaagc gcctgaataa tcagatacac cgcgaaacag
      421 ctccagaatt cagtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccagg tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggcaccagca ccagatagac ggctatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg catccattaa cgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 attaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatcagc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacgat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcatttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ctgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 agcacaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 tgatatcgat cttgggcaaa acaaagccaa tgaggcgatc aatcgtctga aaaatgcccg
     2581 cgatttacgc accaatccgg tgttgcagct caacctggcg aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattcttaa tcgctacacc tttaataata aagatgacag
     2701 taacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggaact
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cgggcgactc gatcaggcta tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagcctgcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttacgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaagaa aacggcgtgg agccagaagt ggtgctctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag aatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gctccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccacgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcaccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca atttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatgcc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca accgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcgg ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac tgatcagttt
     4321 gaaaatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcaactccgc
     4381 cgcgccgatg tttaccttcg cgccactgac gccgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt cgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccgcc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacggtc gacaggccgt tagcaaacat
     4741 cgaacggtac aaacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtagta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaa
//
LOCUS       DAZUGK010000248         5528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZUGK010000248 DAZUGK010000000
VERSION     DAZUGK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.250"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            113..559
                     /gene="hpaR"
                     /locus_tag="ACHY5W_004253"
     CDS             113..559
                     /gene="hpaR"
                     /locus_tag="ACHY5W_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFS4449017.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            678..2342
                     /gene="tsr"
                     /locus_tag="ACHY5W_004254"
     CDS             678..2342
                     /gene="tsr"
                     /locus_tag="ACHY5W_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFS4449018.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAALRKMAVADSGENWETF"
     gene            complement(2385..3656)
                     /locus_tag="ACHY5W_004255"
     CDS             complement(2385..3656)
                     /locus_tag="ACHY5W_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4449019.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3653..4126)
                     /locus_tag="ACHY5W_004256"
     CDS             complement(3653..4126)
                     /locus_tag="ACHY5W_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4449020.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKHRRVIAVLVAVLSITLLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGEKMQ"
     gene            complement(4190..5161)
                     /locus_tag="ACHY5W_004257"
     CDS             complement(4190..5161)
                     /locus_tag="ACHY5W_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFS4449021.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1463 a         1294 c         1405 g         1366 t
ORIGIN      
        1 aaaatgtcat caattttgat aaaatcagga attgcgagga cagctcagtc ccggtaacgt
       61 gacgattaac tgtttctata ttaatgattt tgaatttttc aggggaaaga taatgcacga
      121 ctcactaact attgcgttgc tccaggcgcg agaagcggcg atgagttatt ttcgcccaat
      181 cgttaaacgg cataatttga ctgagcaaca gtggcgcatc gtgcgtattc tggcggaaag
      241 cccatcaatg gattttcacg atctggcgta tcgcgcctgc attttgcgcc caagcctgac
      301 cgggatcctc acgcggatgg aacgcgacgg tttagtgttg cgattaaagc cgatcaatga
      361 tcagcggaag ctctatatct cgctaaccaa agaggggcag gcgctgtata accgcgccca
      421 gacacagatt gaagaagcct atcggcagat tgaagctcag tttactgccg agaaaatgca
      481 acagttaacc catctgttag aggaatttat tgctctgggc aattcccgac aagaagacat
      541 cccgggcgat aatgaataac tttttagtat ctgataacca gcgcctggtt tttccatgga
      601 tggcgggtta caccttttca taaagttttt gctttccagg ccgaaaatct tgcatcggtc
      661 cacaggaaag agaaaccatg ttaaaacgta tcaagatcgt gaccagctta ctgctggttt
      721 tggccgtttt tggcctttta caactgacat caggcggtct gttctttaat gccttaaaga
      781 atgacaaaga aaatttcact gttttacaaa ccattcgcca gcagcaatcc acgctgaatg
      841 gcagctgggt cgcgttgttg cagacgcgta acaccctcaa ccgcgcgggt atccgctaca
      901 tgatggatca gaataatatt ggtagcggtt caaccgttgc tgagctgatg cagagtgcca
      961 gtatttcgct gaaacaggcg gaaaaaaact gggcggatta cgaagcgttg ccgcgtgacc
     1021 cgcgtcagag caccgccgca gcggcagaga tcaaacgtaa ttacgatatt tatcacaatg
     1081 cgctggcgga gctgatccaa ctgttaggtg caggcaaaat caacgagttc tttgatcagc
     1141 caacccaggg atatcaggac ggtttcgaga agcagtatgt ggcttacatg gagcaaaacg
     1201 atcggctcta tgatatcgcc gtcagcgata acaatgcctc ctacagccag gcgatgtgga
     1261 ttctggtggg cgtgatgatc gtcgtactgg cggtcatctt cgccgtctgg ttcggtatta
     1321 aagcctcgct ggtagcgcca atgaatcgtc tgattgacag cattcgtcat attgcaggcg
     1381 gcgatctggt gaaaccaatt gaggtggatg gctctaatga gatggggcaa ctggcagaga
     1441 gtttgcgcca tatgcaggga gagctgatgc gtaccgtcgg tgatgtgcgc aacggggcca
     1501 atgccattta tagcggtgcc agcgaaatcg ccaccggcaa taacgatctc tcttcgcgca
     1561 ccgagcaaca ggccgcttcg ctggaagaga cggcagccag catggagcaa ctgaccgcaa
     1621 cggtgaaaca gaacgccgag aatgcgcgcc aggccagcca cctggcgtta agtgcttctg
     1681 aaacggcgca acgcggcggc aaagtggtag ataacgtggt gcagactatg cgtgatatct
     1741 ccaccagttc gcagaaaatc gccgatatta tcagcgtaat tgacggcatt gccttccaga
     1801 ccaatattct ggctttgaac gcggcggttg aagcagcgcg tgcgggtgag caaggacgtg
     1861 gttttgcggt ggttgcggga gaagtgcgta atctggccca gcgtagcgct caggcggctc
     1921 gtgaaattaa aagcctgatt gaagactcgg tggggaaagt ggatgttggc tctacgctgg
     1981 tcgaaagcgc cggggaaaca atggcggaga ttgtcagtgc cgtgacccgc gtgacggaca
     2041 ttatgggcga aatagcttct gcttctgatg agcagagccg tggtatcgat caggttggct
     2101 tagcggttgc tgagatggac cgggtaactc aacagaacgc cgcgctggtg gaagaatctg
     2161 ccgccgccgc cgccgcgctg gaagagcagg ccagtcgcct gaccgaagct gtggcagtgt
     2221 tccggattca gcaacagcag caacagcagc gtgaaacatc ggctgtggta aaaaccgtga
     2281 cgccagctgc gctgcgtaaa atggccgtgg cagatagcgg ggagaactgg gaaacgtttt
     2341 aacgcaatga cgccggatga gtatttcatc cggcgttttt attattaaac caggaacagt
     2401 gaaatcgccg ggatatatgt caccagcatt agcattaata gtagagccgc cagtagaggc
     2461 agggattgtt taaagaaatt tcccaccgaa acttgcgtga tgttacatac cagcagcatc
     2521 acggtaccta ccggtggcgt gaatgcgcca atcgacaggt tgaagatcat gatgatgcca
     2581 aaatgcaccg gatcgatgcc aaagtgttcg accaccggtt ttaatagtgg taccaggatc
     2641 agcaaaatgg cgtttccttc cagaaacatc cccagcgtca gcagcaatat gttgataatt
     2701 aacaggaaca tatatttatt ttcggtcagc gaagtcacaa aattcgccac tgcgacacca
     2761 gcatgttcca ttgagaaaat ccatcccaac gcggcgctgg ccatgattac cagcatcacc
     2821 gatgcggttg cgcgtgccgt ttcgcccaat gctttgacga catgagaaat ccgcatttca
     2881 cgatagataa aaaagccaat cgccagcacg attagcaccg ctgctgcacc tgcttctgtt
     2941 ggggtgaaaa tgttagcgcg aataccaccc accacagcaa aaatgaggaa cagcgcaggc
     3001 caggcctgcc ccagcgagaa caccacctct ttgcccgtag gccagctttc acgcgagggt
     3061 ttagcgttag ttttgcgggc gaccaggtag acggtaaaca tcaggacgac tgcgcatagc
     3121 aaaccaggta ccagaccggc gataaacatt tttccgatag agacgtcggc aaccagaccg
     3181 taaataatca gggctattcc cggcggtatg atcggggtga ctaacgagcc accagcggta
     3241 atggcggcgg caaatgcgcg attgtagcct ttacgctcca tctccggcac catcatgcgc
     3301 gacagcatcg ccgcgtcagc aaggttagag gcgctgacgc cgcccatcat ggtgctgacg
     3361 agaatgtttg ccacgccaag tccgccgcgc attttcccca ccagaacgtt ggcaatgtgc
     3421 aggatgcgtt cggcaatgcc ggtgtagctc attagtgtgc cgagcaagat aaaaaacgga
     3481 attgccagca atgatgggct ttgcgttggc gcgatcagtc tttgcacggc aatttcaatc
     3541 ggtacgctgg agaagaataa aaagtaagcg atgatgccaa taaacatcgc cagatagacc
     3601 cgaatgttga gaaacaaaca gacgatcatt agcggaataa tcaataacca actcattgca
     3661 ttttttctcc gctatcggat tgttttatat cgtgtattaa ccagtagagg gtataaatag
     3721 ccgttgccag tgcgccgaca gggaccgcca gatcgatcca gaaccaggaa accttcagaa
     3781 tttgtgtcac tttaaactgc gccatttcgg ctagtcggta gcccagccag gccatataca
     3841 gcagcagtgt gatggagaga acggcgacca gtactgcaat aacacgcctg tgtttgcctg
     3901 gaagcatctc catcaggaag ggaatggtca ggtgcgccct gtcacgttcc gccaccactc
     3961 cacctaacat caccacccaa atcatcagca ttcccgacat ttcttctgtc cattgaagcg
     4021 gttggccgat gaaataacgc ataaataccg ctccaattgt catcacaacc agaacgaata
     4081 gcgcaaaggc agcactaaat tctgccactt tgccaaaaag acgcatgaaa cctcctgtta
     4141 gtgcgtgtcc tgcctaagag agatgaggca ggacgctgag cattaatgct tattgcagtt
     4201 gctgttgaat ggtttcgtac agacctggtg tccattccgg gaattgagag taaacttcgc
     4261 gtgctttctt ctggaatggt ccagtatcgg gatagatcac ttcgacgccc gctgcctgca
     4321 tggttttcaa catttcggca tcacgctcaa gagtgagttt ctggctatag acacccgctt
     4381 catagccggt ctggtggatc atctccagtt gttctggtga aagggtgcta aagaaggctt
     4441 caccacctat ccatactgag gtatttgtca ggtagttaac cattgtcaga tatcttgcct
     4501 gctcatacag tttttgccct tgcagcactg aaattgggtt ttctacgccg tcaatgacgc
     4561 cctgggttaa tgcgggataa acttcgccta atggcatggg agttggtgtt gcccccatcg
     4621 cctgaatggc ttttatttgc atgacgttat taggaacgcg aatttttaag cctgcgagat
     4681 cgtcaacggt gcgaatgggt tttttcgaaa taatctgtcg agtgccatag agataattat
     4741 tcattaccac atgaattccc tttttcttca gactttcatt tttttgttta aaccagtcgc
     4801 tttcatatat tttgaacagt ttttgtggat cgtcagtcag atacggtcca aacaaaatac
     4861 ccaaatcggg ttcataatcg gcaagaaagg ccacatcact caaggttatg acattcatgc
     4921 ccatcatggc ttgctcggtg acatcctgtt ttgagcccaa ttgggaactt ggatagagcg
     4981 caagtgttat ttcgccatta ctttttttat ttaataggtc tgcccagtaa cgcatgacca
     5041 catccagggg ttctccaggg ttattttcat aggcaacttt tatcgaaata ggttttgcct
     5101 ggattattga cggtaaaaaa agggtactga cagtaattag catcgctatt attttgctca
     5161 tctttactta ctccatgcta aggaaataaa actccaacat tgttgtcggg agtgaaacct
     5221 tatgaaaaag cagtcattac tgaaaagtag tcagcgaata gcggaatggc tgcagatgtg
     5281 tcataaattc gtgcaccggg taatactttg ctgcttttaa tcggttttta atgcattttt
     5341 tgattgtata tcttaaagtt atgtaatgca tgacgttaca aggaacaccc tctgatgagt
     5401 ttagttaccg ctgataagat ctcttattcg gttgatgatt tattaaaaat aaaatcatta
     5461 atttggctgt ggctattttt acagataaaa actaagtgtt gacgcagaat ggtggtatcc
     5521 attaaatt
//
LOCUS       DAZUGK010000249         5515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZUGK010000249 DAZUGK010000000
VERSION     DAZUGK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.251"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..312
                     /locus_tag="ACHY5W_004258"
     CDS             109..312
                     /locus_tag="ACHY5W_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449022.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            372..953
                     /locus_tag="ACHY5W_004259"
     CDS             372..953
                     /locus_tag="ACHY5W_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFS4449023.1"
                     /translation="MNKNIAVTGKGDARHVKKICDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCVALEISNSRDALKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRCKEQYQLSASQKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGNRIQ"
     gene            complement(1074..1253)
                     /locus_tag="ACHY5W_004260"
     CDS             complement(1074..1253)
                     /locus_tag="ACHY5W_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4449024.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            1450..1641
                     /locus_tag="ACHY5W_004261"
     CDS             1450..1641
                     /locus_tag="ACHY5W_004261"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4449025.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     CQQSPDGCVMVFAARIRQG"
     gene            1645..2010
                     /locus_tag="ACHY5W_004262"
     CDS             1645..2010
                     /locus_tag="ACHY5W_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449026.1"
                     /translation="MHNLSISDLNCIQFDEKFTGQLLVHVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPLTNHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
     gene            2003..2236
                     /locus_tag="ACHY5W_004263"
     CDS             2003..2236
                     /locus_tag="ACHY5W_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449027.1"
                     /translation="MRDDRFNSLKREFDGAQEDAAGALLSVAGMMKAAYFLINTSGYK
                     SEGEMILSIASDYAEYVAETRYRRKSTEDVSHA"
     gene            2229..2429
                     /locus_tag="ACHY5W_004264"
     CDS             2229..2429
                     /locus_tag="ACHY5W_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449028.1"
                     /translation="MHNHEAHVPVVLNVPDDFTGRVLVYLDKGKVKSQCRLKSNEIVG
                     SPEFFSELCIRAEIKPELLTGK"
     gene            2434..2724
                     /locus_tag="ACHY5W_004265"
     CDS             2434..2724
                     /locus_tag="ACHY5W_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4449029.1"
                     /translation="MKKKNSGFTASGLSRPEIRHGDIYRDTRRGGRVVIRHVTPGNIT
                     YRREAYEYDCVMPRRQFERDFILVENKQQAVARRAATNIKKIRAMLVAGGKK"
     gene            2721..4526
                     /locus_tag="ACHY5W_004266"
     CDS             2721..4526
                     /locus_tag="ACHY5W_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFS4449030.1"
                     /translation="MKLAPNLKKQPHDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGDSQLDELADLKIIDDGRYCVRLYKAGHIKPSNINAIGQKLAVAGVRDANYY
                     PEGMHSQKRENWHEYLERERQNLSDGLVIQLPVKKKTEDSAAPLALNQMGASQRGEVL
                     LARYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIKSAVD
                     TMKLSLPVMGNAARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFSPPAEGETLA
                     THAPNFWKWLRRSVAENDRKADRVLAALFMVLANRCDWQLFLEVTGAGGSGKSVMAEI
                     CTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGDKVA
                     IDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPMLAE
                     KIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMSSVM
                     CDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGREYM
                     KRKTKHGLRSNVTLTEDSEDWMPSCASVTNDDGKN"
     gene            4814..5059
                     /locus_tag="ACHY5W_004267"
     CDS             4814..5059
                     /locus_tag="ACHY5W_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449031.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            5056..5478
                     /locus_tag="ACHY5W_004268"
     CDS             5056..5478
                     /locus_tag="ACHY5W_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4449032.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVMAFGRQAEALERHRKGELLSVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RTARLGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         1534 a         1167 c         1528 g         1286 t
ORIGIN      
        1 tgatgcaatg ggtcggtgat ttttttgatg atgcgagaaa aggggtaatt aatagatccg
       61 gtggtatgaa aggtttaaga gtagtaaatg gttgaggagg ttcagcaaat gaataccaat
      121 gaagatattt tatttactaa agacgtaatg aaaatcttgc gatatggagc aatgagtgca
      181 ttcatcaatt tctggaaaga tgagaataat ggttttcctc agccgttcag aattggacga
      241 cgacatacct ggcaccgtag agatgtagaa gcatggttag ataaacaacg agaacaagcc
      301 aatccccact aataatatct ttcatacccc gcgtgcaatg cggggttttt tgtatgtgag
      361 gtaaaaaaga aatgaataaa aatattgccg tgacgggcaa gggtgacgca cgtcatgtaa
      421 aaaaaatctg tgattttcgt gatctggtcg ttctgcgctt tgatagtgtg aacgttcgcg
      481 tggtgtatct gaacggcgat ccgtggtttg ttgcaaagga tgtttgtgtt gctctggaaa
      541 taagtaactc acgcgatgct ctaaaagcac tagatgcgga tgaaaagaaa accgtcgctt
      601 taagctacgg tattcgagga aatccaaatc atagcctcat ttctgaatcc ggtttttaca
      661 agctgatcgc cagaagtcgg aaagctgtta ctcctggcac gttcgcccat cgtttcagta
      721 actgggtatt caggaatgtg ataccaggaa tcagaaaaac gggggcttat ggtatcccgt
      781 ggggcgcatt acaggacttt tctcgctgta aagagcaata tcagttaagt gccagtcaga
      841 aggggaggga gctacaggca tgtaagcgca aaaagcgtga gctggaggaa gaagaaaaaa
      901 ggctgatacg tgaatatcag cctgagtttt actttggcaa ccgcattcag tgacaaaaca
      961 aaggcgaccc ggatcagagg tcgccaatgg gaaaacacta aacataagcc caaacatcat
     1021 agcgatttgt tggctggtgg gcaatgtgat cagtgtgctg gcttgctggt ggactattcc
     1081 gcttctttgg ccttgcggcg ctggcggcgt ttgatctcgc ctcgcatggc tgtaacgata
     1141 aattgcgcag tgctctcgcc tgtttctttt acttgctcca tagcctcaac aacttcatgc
     1201 ggggcgcgag cctgtaattt ctgtgatttg ttattgatat ggtctctttc catttctggt
     1261 tccctttgtg attactggaa tccagtatac acgaaaaaga ccaaaacaaa acgcttgaag
     1321 tggattccac ttagtgatta tactggaatc cagttgagtg tttatcactc aatgcaaacg
     1381 aggctgaaaa ggattgccgt cctttcgcag cctctgacca ccaacgatag caacagtatc
     1441 gaggtagcta tgagaaatca taccacacac ccgcaagggc gggacttgca caacctgaat
     1501 aaattcacct ggcgttttat cgccctgagc acggcacaac cgcgcgtgat taccattgag
     1561 gccaccagcg aacaggaagc atgccagcaa tctcctgatg gctgcgtgat ggtattcgct
     1621 gcccgtattc gtcaggggta agtcatgcat aacctgtcaa tttctgacct taactgcatt
     1681 cagtttgacg agaaatttac cgggcagcta ctggtccatg tggagaacgg gcgcatagtg
     1741 cgtaattacc acctgccgga tggtgcaatt gccggaagcg ttgaagcatt gctggaactg
     1801 gcagaacgtg cgcgactgat taagccgtta acgaaccatc acgatgatga tctgcatttt
     1861 accggacgca tggtgagtca ctacgaaaac ggcgtcgagg tatcccgcga acggctgcgt
     1921 gatgattgct gtttcggcac actgccggaa tttatcgagt tgctgaccag ttgcgggtat
     1981 caggtcattc aggggggtaa acatgcgtga tgatcgtttt aattccctga aacgggaatt
     2041 tgatggcgca caggaagatg cagcgggcgc attgttgagc gttgctggca tgatgaaagc
     2101 tgcatatttt cttatcaata ccagtggcta caagtcagag ggtgaaatga ttcttagtat
     2161 tgcgtcagac tatgcggaat atgtggcaga gacgcgttac agaagaaaat ccacggagga
     2221 tgtaagccat gcataatcat gaagcgcatg tacccgtagt gcttaatgtg ccagatgatt
     2281 tcaccggacg cgtactggtt tacctggata aagggaaagt gaaatcacaa tgccgactga
     2341 aaagtaatga gattgttggt tctcctgaat ttttttctga actttgtatt cgtgcggaaa
     2401 taaaaccgga actgctgaca ggaaaataaa accatgaaaa agaaaaattc tggctttact
     2461 gccagcggtc tctctcggcc tgaaatcaga cacggagata tttaccgcga caccagacgt
     2521 gggggacgag tggttattcg tcacgttacg ccaggcaata tcacctaccg ccgtgaggct
     2581 tacgaatatg actgcgtaat gccgcgccgt cagtttgagc gtgattttat tctggttgaa
     2641 aacaaacaac aggcggtggc gagacgtgca gccacgaata ttaaaaaaat ccgggcaatg
     2701 ttggttgcag ggggtaagaa atgaaactgg caccgaactt aaaaaaacag ccacacgaca
     2761 aaatgaccga agtcattatt tttgcgggta gtgatgcctg ggcgcacgcg aaacaatggc
     2821 aggaacagga cgggcgactt gctggcgaca acgtgccacc tgtatggctt ggagacagcc
     2881 agcttgacga actggcagac ctgaaaatta tcgacgatgg tcgctattgt gtccggctgt
     2941 acaaggcagg ccacatcaag ccgtcaaata ttaatgccat cgggcaaaag ctggcggtgg
     3001 caggtgtacg ggatgcgaat tattaccctg agggaatgca cagccagaag cgggagaact
     3061 ggcacgaata tctggagcgt gagcgccaga atctttctga tggtctggtt attcagcttc
     3121 ctgttaagaa aaaaacagaa gacagcgccg caccactggc gcttaaccag atgggagcaa
     3181 gccagcgcgg cgaagttctc ctggcacgtt atggcggtga actggcaatc aatgcggact
     3241 ctgacaccgt tcaccactac aacggcgttg tatgggagcc agtacaggat aaagagttac
     3301 agcgggctat ggcgcagatt tttattgatg cggagatcag ttattcgcag aacgccatca
     3361 aatcggcggt cgataccatg aagttaagtt tgcccgtaat ggggaatgcc gcccgtaacc
     3421 tgattggatt cagtaacggt gtttttgata cccggacagg cgattttcgg gagcatgaca
     3481 aaaacgactg gctgttaatt gccagtgaat taccgttcag cccgccagca gagggggaaa
     3541 cgctggcaac gcatgcgccg aatttctgga aatggttgcg ccgttcggtg gcagagaatg
     3601 accgtaaagc ggatcgcgta ctggcggcgt tattcatggt gctggcgaac cgatgcgact
     3661 ggcagttatt tcttgaggta acgggggcgg gtggaagcgg taaaagcgta atggcagaga
     3721 tttgcaccat gctggcgggt aaggccaaca cggtatcggc aagcatgaag gcgctggaag
     3781 atgccaggga acgcgcgtta gtggttggct tttcgctgat tatcatgccg gatatgaccc
     3841 gctacgctgg tgatggcgca ggaattaagg ccattacagg cggtgacaag gtggcaatcg
     3901 acccgaagca taaagccccc tactcaacac gcattcaggc ggtcgtgttg gcggtgaaca
     3961 ataacgccat gtcattcagt gaccgcagcg gggggatctc acgtcgtcgg gtgatattca
     4021 atttttcgga ggttgtaccg gaaaacgaac gcgaccccat gctggcagaa aaaatagaag
     4081 gagagctggc ggttgtgatt cgtcacctgc ttactcgttt ttctgaccag gacgaagcta
     4141 aacgcctgct gtatgagcag cagaaatcag aagaagctct ggtgataaaa cgcgagggcg
     4201 attcgctggt ggacttctgc ggctatctca tgtcgtcggt aatgtgtgat ggtctgttag
     4261 tgggtaatgc cgaaattatt ccgttcagtc cgcgcaggta tctttatcac gcctatctgg
     4321 catatatgag ggcacatgga ttcggtaaac ctgtaacact gacgcgcttc ggtaaagata
     4381 tgccgggggc aatggcggaa tatggcaggg agtatatgaa acggaaaacg aagcacggtt
     4441 tgcgttcaaa tgtgaccctg acagaggatt cagaagactg gatgccatca tgtgcatcgg
     4501 tcacaaatga cgatggaaaa aattaaactt atggaataac tgttcaccac tgttcaccct
     4561 gcaataaatg tcttttatat cagtatatta tagggtgagc agttatttat gaactgttca
     4621 ccaaactatt cactgttcac ctttttgatt ctttattgag ctttaagggt gaacagtggt
     4681 gaacagttgg tgaatagttt ttgtgaaact gttcacccct taacattatg aataaaaagg
     4741 gaaaattgca aaaggtgaac aggtgaaggg ttgaaatgca aaaattttat tttattgctg
     4801 tgaggtaaag cctgtgacaa cgaagcacgc caaaaaacca caatctcacg cccttgattt
     4861 gacagaacac tggctgaggg tggcgataaa aatcatcgac cgcaacgccg gggaaggata
     4921 cgcgaaagca catcccgaac tgataagcgc attcatgacc acgacggcgg caaactttgc
     4981 cacgctgaca gaacgggaga ttgccgaagc ggaacaggta acaaccatca acgttaaaac
     5041 cggagaggtg gaatcatgac agcacagata gcagcttacg ggcggctggt ggacgacccg
     5101 caggtaaaac agaccagcaa gggcacacca atgacgctgg cacgtatggc ggtatcgttg
     5161 ccatgtagtc aggcgcagga tgggcaggct acgttatggc tatcggtgat ggcatttggc
     5221 agacaggccg aagcactgga aaggcaccgc aagggtgaac tcctgagcgt ggcgggtaac
     5281 atgcagatca gccagtggac tgggcagaac ggagaaacgc ggcagggctg gcaggttatc
     5341 gcagacagtg taatcagtgc gcgaacggcg cgactgggcg gcaaaaaagg tcaacagggc
     5401 caggctactg acgcgctgaa cagggcaaaa caacaggcgg ggaatgatga tccgtacggg
     5461 gataacatac cgttttaagc gacgagtgac agaagccgga gcaatccggc ttttt
//
LOCUS       DAZUGK010000250         5499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZUGK010000250 DAZUGK010000000
VERSION     DAZUGK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.252"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            206..265
                     /gene="ypfM"
                     /locus_tag="ACHY5W_004269"
     CDS             206..265
                     /gene="ypfM"
                     /locus_tag="ACHY5W_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFS4449033.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(364..3477)
                     /gene="acrD"
                     /locus_tag="ACHY5W_004270"
     CDS             complement(364..3477)
                     /gene="acrD"
                     /locus_tag="ACHY5W_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFS4449034.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(3641..5341)
                     /gene="narQ"
                     /locus_tag="ACHY5W_004271"
     CDS             complement(3641..5341)
                     /gene="narQ"
                     /locus_tag="ACHY5W_004271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFS4449035.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
BASE COUNT         1370 a         1466 c         1381 g         1282 t
ORIGIN      
        1 tattttaacc agaattaaat cagtattaga tgccgtatga atatctcgaa atgttgctgt
       61 gtatcacata caaatcatca ccgcaggacc atagtattta cataaggttt gtcccccgga
      121 ggtagtcatc gcagcaatcg agtaaattag cttcaccacg gtatattgtt tccgtagaat
      181 acccataatt ataagagagg ttgttatgat tgaacgtgaa ctggggaact ggaaagactt
      241 tatcgaagtt atgcttcgta agtaattcct gagacaaagt acaaacagca agaacccgct
      301 gagcaggttc ttaatcggaa agttgaaaaa ggcgacacat tagcatgtcg ccttttttat
      361 tgcttattcc gggcgcggct tcagcgggaa gcggcggcgc accagcacaa agaacagcgg
      421 cacgaagtaa atagccagga tagtggccga aatcatcccg cccattacgc cagtacccac
      481 cgcatgctga ccaccggaac cggcacccgt gctggttgcc attggcaata cgccgaagat
      541 aaatgccagc gaggtcatca gaatcgggcg taaacgctga cggcaggcgt ggagcgtcgc
      601 ttcaaacagg tcgtggcctt tttggttcat ctcgttagca aactcgacga tcaggatggc
      661 gtttttcgcc gataaaccaa tgaccgttaa caggcccacc tggaagtaaa cgtcgttttc
      721 cagcccgcgc atccaggttg ccagcagcgc gccgataacc cccagcggca cgaccagcat
      781 taccgagaac ggtaccgacc agctttcata caatgccgcc agacacagga ataccaccag
      841 caaggaaatg gcgtacagcg ccggagcctg cgcgccggaa agccgctcct gatacgacat
      901 cgccgtccac tccagaccaa agccgtttgg cagctgcttc actaacgatt ccataatatc
      961 catcgccgta ccggtactga cccccggtgc ggcttcccca acaatttcaa ccgcagaata
     1021 accgttatag cgttccagac gcggcgagcc tgtctcccag cgtgaggtcg cgaaagcaga
     1081 gaagggcacc atgccgccat ctttatttcg gacataccag agattgatgt catctggcag
     1141 catgcgatac ggcgcagctg cctgcacata gactttcttc acgcgaccgc gatccataaa
     1201 gtcattcaca tagctcgaac cccaggcggt ttgcagtgtg tcgttaatat cgtcgatagc
     1261 aacgcccagc gcctgagctt tacgctggtc gatatcaatc tgcaactgcg gactgtcatc
     1321 gaggccgtta tggcgcacac gggttagctc cgggttttcc gccgccagcg ccagcaactg
     1381 gttacgtgct gccatcagcg catcgtgacc tgctccagcg tggtcctgca actccatatc
     1441 aaaacctgca gaactaccaa gaccgctaat tgctggcggg ctgctggcga taacgcgagc
     1501 ttctttaatt tggttaaacg cctttgttgc acgctcgata atggcaaacg aggtgccggt
     1561 cttactgtcg cgttcgctcc agtctttcag gcggataaac attcgcgcca cgttttgccc
     1621 gttaccccca gggccagaac caacagtggc aaacaccgac atgatgttgt ctttttcatg
     1681 ggtgaagtaa tatttctcga tttgctcaac gactttcagg gtctgttgtt gggttgaacc
     1741 gctgggcaat tgtaccgagg tagtaaacat gccccggtct tccagcggta agaacgacgt
     1801 cggcaaacgc aggaacagga acaccatgcc gccaagcagc aggacataaa tcacaatcca
     1861 gcgcaggctg cggtggagaa ttttcgccac ccctttttcg tagcgttcgg cgttgcggtt
     1921 aaacatctgg ttaaaccagg caaaaaagcc tttttgcccg tgatgctcac cttttttcag
     1981 tggcttaagt agtgtggcac acagagccgg agtgaggatc atcgctacca gtactgacag
     2041 caccatcgcc gcaacaatgg taatagagaa ctggcgatag atggcaccgg tggtgccgcc
     2101 gaagaaggcc attggtacaa ataccgccga aagcaccatc gcaatcccga ccagtgcccc
     2161 ttggatctgc cccatcgatt tacgtgtggc ttcgcgagga gtgagtcctt cctcactcat
     2221 aatacgttcg acgttttcca ccaccacgat ggcgtcatcc accagcagac cgatcgccag
     2281 taccatcgcg aacatggtta aggtgttgac gctgtaaccg aaggcgtaaa gtacggagaa
     2341 ggttcccatc aacaccaccg gcacggcgat agtgggtatc agcgtggcgc ggaagttttg
     2401 caggaacaga tacataacga ggaaaaccag ggcgatagct tccagcagcg ttttcaccac
     2461 gtcttcaatg gaggctttaa caaacgaggt ggtttcatac gccaccttgt attccagtcc
     2521 gtgcgggaag tactgcgcca gctcgtcgag acgattgagc accagctccg ctgtcgccat
     2581 ttcgtttgcg ccggaggcca gttttacccc cagcccggag gctggcttac cattgaagcg
     2641 gctaagataa tcgtatttct ccgcccccat ttcgacggtg gcgacatcgc ccagccttac
     2701 ctctgagccg tcttgattca cccgcaaggt gatatcgcgg aactgttctg gtgtttgcag
     2761 cagtgactgg gcgttaatgg tggcgttgag cgcctgctta tcgacggaag gtgtaccacc
     2821 aagttgccca accgcaatct gcgcgttctg tgactcaatg gcatcagtga catctttagc
     2881 cgtcatctgg aaactgttga gtttcgccgg gtccagccag atacgcatgg aatattgcga
     2941 accataggca tcgatatccc cgacgccatt cacgcggctt aacgggtcct gaatattact
     3001 ggcaacataa tcggcaatat cctgtttatc catcgaacca tcggtagaga cgaaagcaat
     3061 ggtcagaata ttggtatcgc cggttttacg caccgtcacg ccctgatttt gcaccgcctg
     3121 cggtaacttt cgcatggctg attgcagctg gttttgtact tgctgcacgg cttcgtccgg
     3181 atcggtgcct gctttaaaac ttaaagtgac agatgcctga ccggtgccac tgctctgaga
     3241 tgacatatac atgagattat cgaggccggt catattttgc tcgataacct gggtcacggt
     3301 gttttccagc gtctgggccg atgcgccggg atagttagcg gtcactcgca cattcggtgg
     3361 cgcgagatcg gggtattgtt caacgggcaa tgaaaaaatc gccagggtcc ctgtcagaca
     3421 caacaggatt gccagcaccc aggcaaaaat ggggcgatca ataaagaaat tcgccattaa
     3481 aagaggacct cgtgtttctg cgtatcgttg taggcttgac ttgcttcact gtagcggcaa
     3541 ggtacgagcc aatcgtggag aaaaaaagga gttaatgtaa attatcatgc ccttttatac
     3601 tgaaccttaa gtgcaagtat tctttggtca gtaggaggca ttacatcaac tgactttcct
     3661 caccctccgc agagcgaaag ctaatactca ctaacgtgcc gccgccggaa ggttgtgaaa
     3721 aagtcagcgt cccacctagc cgttccgcgc gttcgcgcat gatattcaga ccataatgac
     3781 cttcgggttc tttcggttca ccgataccaa tcccgttatc acggatataa accgtgtgat
     3841 tgccgtccgg cgcggtgacg caactgacgg cgatttcgct ggcgttggca tgcttcatcg
     3901 cattcagcac cgcttcgcga ataatttgca acaaatgcac ctgcatttgc gcatccagcg
     3961 ccagggttgg cagacggcag tcgagggtca gtttggcgct ggtttgattt tgtaacgtat
     4021 ccagcatttc cctcaacgcg gaggggagat ccgcctgctg cagcgtcagg cggaaagtag
     4081 tcaacagctc gcgtaactgc cgataagcat cattcaacgc ctgggaaaaa tcggccatga
     4141 tactttgtgc ggtggcgtta tcttccggta tcgaacgctt aagtaacgtc aactggatac
     4201 gtaagtaaga aagcacctga gccagcgagt cgtgcaattc gcgggcgatg gtcgcacgtt
     4261 cttccatcaa caataactgc tgaaaatgct tctgcgcctg attaaagtac aaaccgcgtc
     4321 ccagcatcga tgaaacgctg ttaagcagcg gttctgatga tgaaacgtga ctattttgcc
     4381 agtgcagttc accgtaaacc gtctcttgca ttgtcaccgg taaaatctgc atcggcaatt
     4441 ccgggtttgg ttgcccttcg ctaatccgcc agttttcacc gacatttaac tccagatact
     4501 cagccgcttc attgtcgcga acaatctgca aaatatggcg gaaacaatgc acatcaatct
     4561 ggctggtgtt cagcgcctgc gaacattgat acaacacctc cagacgacgc ttggcctcgt
     4621 ggagatcgcg ggtcttttct tctactgacg cctccagcga aaggtacaat ttatgcagct
     4681 cgctcgacat ctggttaaag gtttttgcaa gcaggccaag ctcattcggc aggttggtat
     4741 ccagcggcgg cgagtcgaac tgcccgtgtt caatacgctg actggcggta accagctgat
     4801 tcagcggggc aaccacctga tggcgtatac ggcgcaaagt aaaaaagacc agcgtgaaaa
     4861 taccgatgcc gccagccaag gaaatcgcca ccaccagcag cattttgcgt tcagcgtagt
     4921 gctgtaaagc cagcacgaac aggtctatct gattaacata attattaata ttggcctgat
     4981 accacggcaa atcgcccttg ctgagccgat tattcatctc cagccagttg gcattcagat
     5041 gcgcatagcg agtttttact gcttctggca cataccagac gttgagattg gttaataccg
     5101 gtgaatgcag tgcctgctga aacaactggc gatgtgcatt gagttgtgga ctgccacttt
     5161 gcaagtcgta gcccaggcgg taactctgca tacgtagcga tccggcaata ttgatagcct
     5221 cagcgtcgcg caaactgctc gccagagtta gcagagcgat acccgtggaa agaatcgaca
     5281 gcagcacaat gtaaaaaaag gcccgggcca gactggccga gacgggtcgt ttaacaatca
     5341 cacgcgtctt ctccacaaaa aatcattaat gttccagttc gctgaaacac aatttagtca
     5401 gtgatgggat tatttgatct gccacagttt atagcgaaat agttacttat tctgggcaca
     5461 tgaacattgc cgattcccct gtaagtcggg taataacaa
//
LOCUS       DAZUGK010000251         5408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZUGK010000251 DAZUGK010000000
VERSION     DAZUGK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.253"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..902
                     /locus_tag="ACHY5W_004272"
     CDS             <1..902
                     /locus_tag="ACHY5W_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFS4449036.1"
                     /translation="SFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSSPGIN
                     LKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITGDNYS
                     SQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHC
                     REYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPLTFDN
                     TALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLL
                     YEQNRVVPVIEGKTLCVAPNFDL"
     gene            1915..2478
                     /locus_tag="ACHY5W_004273"
     CDS             1915..2478
                     /locus_tag="ACHY5W_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS4449037.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     SPSFGHSCRHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFVGYLDGQRHKKS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVMHYSDKSAVDNLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            2633..4993
                     /locus_tag="ACHY5W_004274"
     CDS             2633..4993
                     /locus_tag="ACHY5W_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4449038.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPPISVQRVSV
                     LSTNEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYYHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCNRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRESGGSVFVDMNFQPYSDQWAFLASVIPMNVQNI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEGKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPQGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSGLLRYDTGVLCAPTAFG
                     KTVTAAAVIARRKVNTLILVHRTELLKQWQERLAVFLQVGDNIGIIGGGKHKPCGNID
                     IAVVQSISRHGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLTRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIVADGEGLSF"
     gene            complement(5011..5199)
                     /locus_tag="ACHY5W_004275"
     CDS             complement(5011..5199)
                     /locus_tag="ACHY5W_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449039.1"
                     /translation="MANYQLNEQLLEGCRPWIVIFDDVLTAGSHFKAMKSLILQHIPE
                     ACILGLFVARTTRGAQII"
     gene            complement(5266..>5408)
                     /locus_tag="ACHY5W_004276"
     CDS             complement(5266..>5408)
                     /locus_tag="ACHY5W_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFS4449040.1"
                     /translation="YSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALP
                     AE"
BASE COUNT         1423 a         1052 c         1355 g         1578 t
ORIGIN      
        1 gttcttttgc tcttgtatcc agtgaagggg gagtaatttt tgacagccgg gccatgagta
       61 aactgggaat tattaacact ttgtgggatg gaggttctct tttcatcgac aggaaatcat
      121 ctcccggaat taatttgaag gagccaagac tgacgatgtc ggcgatgatt cagcctgatg
      181 tttaccacaa aggtttttgt acgcgaaaaa aagaacttgt gaaaacgtca ggacatcatg
      241 caaggttttt gatgtgtcaa ccaacatcaa tgcaggggac aaggataata accggagata
      301 attattcatc acagtatcag gagttatttg ataagaggat caatgaactt attgatgaaa
      361 gtctggcgat gagtggtgaa cgacgttgcc ttcacttttc tcctcaggct gccagaatct
      421 ggacggatta ttacaacgat gtggaatcga aactgggggg attggggcct ttaagacatt
      481 gtcgggaata tgctgccaaa aatgcagagt atatggcaag actggcagga cttatttacc
      541 attcgagcgg tgaagagggg gagatttccc cttacactgc agaaatggcg agagaattag
      601 caatatggta cggtaatgag tatatgcggt tgtctaatcc gttaactttt gacaacactg
      661 ctctgaccgt acctgtgcga ctcattccag aggagcttga acttttcaac tggataaaaa
      721 gctattgcat tgagaagggg atcctctgta tgaaaaaaaa tgacatttta cagcgtggtc
      781 cgaaccgttt ccggaaaaag gataaaatca actggttact ggatttactg tatgaacaaa
      841 acagagttgt accggttatt gagggaaaaa cgttgtgtgt cgcacctaac tttgacctct
      901 gaacgactta atcgttgagt aacatatagt ctcctccgtt acttgcggag gagattaatg
      961 ccatgaccat gtcagtgttg tattacagaa ctctggtaca ggtcagtcag taaatataac
     1021 tggtgtaact actatttact attggataga gtgttgtaca ggatattgct ggctttggcc
     1081 ttagataagg agggcacttc attgttttcg tattcaataa ataattaagg ttaattagta
     1141 ttattagtgg aagttatgtt atctgttatg ttgtctcttt ttatcgtaat gtcactcctc
     1201 tttatgctga gtattctgta tgtgcgtaat gcgtctatcc ccatatacta cagtgtggtg
     1261 gtgtatggag tttagttatg tgtaaattat gttactacac tcatagcgta atattgagaa
     1321 agttaatccg ctgaatgtat gtctggtttg catttaacac tggttacgta ccagcacact
     1381 attagagtat attgtcttta gttcgtacgt tatagctccg ttacagttta taagcatggg
     1441 taaatattat cttttttgta tgaaacaatc tttttatggt gtgtagcgta tggtctgtat
     1501 gtcattattc ttttatgcta ctgattaaat atctgtaggc tgctctctgc agagtcgctg
     1561 ttttcactta atggtacgac aatattaata gcgatgttgt ctgtggctat tctatagctg
     1621 acttttgtga atgtcagatt acttcctgta aagaatttag tggtgcttcc tgaaactcaa
     1681 ttctatgagg tgtgtttttc cattgtaatt cgtactggtt ctcagtttaa gagagtctca
     1741 ctgtattctg aagtattacc tcattacagt aatgcggtaa tacaaaacag ttttatcagg
     1801 ttgtctgtgc tgctggttta cttcgtttat taatgccgtg gtacggcgtt tctctactct
     1861 cagtatttac gtaattctct cttcctgtga gttttataaa gtaaggacta taaaatgtat
     1921 aatgccaatc ccaattacaa aatggatttc atgattctga aagacgtgaa tgaacacatg
     1981 gacggtatgt ttcaacgttt ttctaaactg ttacctttca gaattgattt tgcctacagg
     2041 aaagactcgc ccagttttgg tcattcctgt aggcacagta tgtgtatgga aatataccgt
     2101 ttacttagcg aaacccagac gatgctggcg ggatattact gggtaatgga atatacacca
     2161 gacaaagggc ttcatattca ttttgtcggc tacctggatg ggcaacgtca caagaaatct
     2221 tatcagatat ccaggcaact aggagatatc tggaggcgga tcacggaagg ggagggttat
     2281 tttcacctgt gcagggctaa agacaaatac ccggtgcgta tcgatcacgt gatgcactat
     2341 agtgataagt ccgcggtaga caacttacgt tacgccctta gctatctggc taaacaggat
     2401 cagaaagaac acggtattat tctgggccgt agcaggcttc ccgaaaaaag caaccggggc
     2461 cgaccacgac ataactgaat tacagttgta ttaggtatta gaattaagtt tctgtaagtt
     2521 gttgggaggt gcttgagtta ataggcagaa gagggcgggg cgctttgtca tcagtgtagg
     2581 ggacccgaat ttctgccgtg gtgcctcagt ggatcggata caggtaacga atatggttca
     2641 taaatctgac agcgatgaat tagctgcatt gagggcagaa aatgcccgtc tggtctcatt
     2701 acttgaagct catgggattg aatggcgacg taaaccgccg atctctgtgc agcgcgtttc
     2761 tgtattatcc accaatgaga aggttgcatt atttcgtcgg ttgtttcgcg ggcgtgatga
     2821 tgtatgggca cttagatggg aaagtaaaac cagcggcaaa tcagggtact ctcctgcctg
     2881 cgctaacgaa tggcaggcga gaatatgcgg taagccccgg ataaaatgtg gggactgcgc
     2941 tcaccgtcag ttgattcctg tatctgatct cgtcatctac taccatctgg caggtaccca
     3001 tactgtcggg atgtatccgt tgctggaaga tgattcctgt tattttctgg cagttgattt
     3061 tgatgaagct gaatggcaga aggatgcatc cgcattcatg cgatcctgtg atgagctggg
     3121 tgtacccgct gcgctggaaa tatcccgttc acgtcaggga gcgcatgtct ggatattttt
     3181 tgcttcacga gtttcggccc gcgaagctcg ccgtctgggg actgctatta ttagttatac
     3241 gtgtaatcga actcgacagc tgcgattggg ttcttatgac cgattatttc ctaatcagga
     3301 tacgatgcca aaagggggat ttggtaatct catagcatta cctctgcaaa aaagaccgcg
     3361 tgaatcgggg ggaagtgttt ttgttgatat gaatttccag ccttattctg atcaatgggc
     3421 ttttcttgca tcggtgatcc cgatgaatgt gcagaatatt gaaccgacga tattacgggc
     3481 tacaggaagt atccatcctc tggatgtgaa ctttatcaac gaagaagacc tgggtacgcc
     3541 atgggaaggg aaaaaatcat caggaaacag actgaatatt tctattgcag aaccgctgaa
     3601 aatcacgctg gcaaaccaga tctatttcga aaaagcgcaa ttacctcagg tgctgattaa
     3661 ccgacttatt cggctggcag catttccgaa ccctgagttt tataaggctc aggcaatgcg
     3721 tatgtcagtc tggaataagc cccgtgttat aggttgtgcg gagaattacc cgcaacacat
     3781 tgcgttgccc cagggatgtc tggacagcgt attatctttc cttagggaca acaatattgc
     3841 tgcagaatta atcgataaac gatttgccgg gacggaatgt aatgccgttt ttatgggaaa
     3901 cctcagagcg gagcaggaag aggccgtttc gggattactc cgttatgaca ctggtgtgct
     3961 ttgtgcgcca acggcttttg gtaagacagt taccgcagcg gcagtgattg ccagacggaa
     4021 agtgaataca ctgatactgg tacaccggac tgaattgctg aagcagtggc aggagcgtct
     4081 cgcggtgttt ctgcaggttg gtgacaatat tggtattatc gggggaggta aacataaacc
     4141 ctgtggcaat attgatattg cggtggtgca gtccatatcc agacacggag aagttgaacc
     4201 tctggtcagg aattatgggc aaatcattgt ggatgagtgc catcatattg gcgcggtttc
     4261 attttctgcg attctgaagg aaacgaatgc cagatatctg cttggcctga cggcaacacc
     4321 aatccgacgg gatggtctgc atcccattat ttttatgtac tgtggtgcca ttcgccatac
     4381 agcggtccgc ccgaaggaaa gcccacataa tctggaggta ctgacccgtt cccgttttac
     4441 atctggtcat ttaccatcgg atgcgagaat ccaggatatt ttcagagaaa ttgctctgga
     4501 tcatgacaga acggtggcga tagctgaaga agccatgaaa gctttcgggc aggggcgaaa
     4561 agttctggta ctgactgaac gtacagatca tctggatgag atagcatcag tgatgaattc
     4621 actgaaattg tcccccttta ttctccatgg acgactatcg aaaaaaaagc gtgcgatgct
     4681 gatatccggg ctgaatgctc ttcctcctga ttctccccgt attttgttgt caacaggcag
     4741 acttattggt gagggatttg accaccctcc gctggatacg ctgattcttg ccatgcctgt
     4801 gtcatggaaa gggacattac agcagtatgc gggacgtcta cacagagagc ataccggtaa
     4861 aagcgatgtc aggatcattg attttgtgga taccgcgtat ccagtgctgc tcagaatgtg
     4921 ggataaacgc cagcggggtt ataaggcgat ggggtacagg attgtggctg atggtgaagg
     4981 gctttcattt taaggagtac tttacccttt ttaaataatt tgagctcccc gggttgttcg
     5041 ggcaacaaag agtcccagta tacaggcttc aggaatatgt tgtagtatca ggcttttcat
     5101 cgctttaaaa tggctaccgg cagtaagaac gtcatcaaaa ataacaatcc agggcctgca
     5161 tccctccagt aattgttcat tcagttggta gtttgccata atcgcttctg gtgtgggccg
     5221 gtttgttggt aagcgtgcag caagaaccgt attgacgggg atgtgttatt cagctggcag
     5281 tgctatgcgc cacggaagca gttcgctgac ccggttgacc ggccagtctg ctatgacgcc
     5341 aggcacatgg cgaaggtagc tttctggatc cacgtcattc agtttgcacg tcccgatcag
     5401 gctgtaca
//
LOCUS       DAZUGK010000252         5387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZUGK010000252 DAZUGK010000000
VERSION     DAZUGK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.254"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="ACHY5W_004277"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="ACHY5W_004277"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFS4449041.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETQTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="ACHY5W_004278"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="ACHY5W_004278"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFS4449042.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="ACHY5W_004279"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="ACHY5W_004279"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFS4449043.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPSSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="ACHY5W_004280"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="ACHY5W_004280"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFS4449044.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="ACHY5W_004281"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="ACHY5W_004281"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFS4449045.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..>5387
                     /gene="citG"
                     /locus_tag="ACHY5W_004282"
     CDS             4510..>5387
                     /gene="citG"
                     /locus_tag="ACHY5W_004282"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFS4449046.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYLQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1233 a         1529 c         1427 g         1198 t
ORIGIN      
        1 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgtt gaccagcgta ccaggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgat tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc cgggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc tttacgaagg gtcaccgtta tctgattcaa
      661 caggctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccgact ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atttgaagat tttccgtcag
      901 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgcagac tatctccgca
     1021 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc
     1621 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg cattacgtga
     1681 aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt atcgcgatat
     1741 tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg accttgaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatt gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccagcag
     1921 caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacaactg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcattcgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tccggcctgc
     2821 gtggtccact ggcggaagag atctcccgtg gtctgctggc agaaccggtg cagatccact
     2881 ctcacggcgg tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt
     3121 tgatcgtcaa agttgaccgc gttggcgatg ctgcaaaaat cggcgctggc gcgacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact
     3241 ctggctactt caaagaaggt ttctctatgc aaaccggtac cggtggcgca tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gcggtattac cgcgacgatg gttgatctgc acgaaaaagg tctaatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc cacgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg gttcgaaagg tgcatcagtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg gtggataacg
     3721 tgctgacctg catcacccca ggctccagtg tcgatattct ggtcacagac cacggtatcg
     3781 cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgc gaacgcgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgcctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atggcgtgac cgccttgtgt gccttagccg
     4201 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag
     4441 ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac
     4501 tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4561 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg
     4621 aaaccaggcc tcgtggatcg gattaactgc ggtgcgcaca aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc
     4741 tgtagtgcag aaatggcacc agaagcggta ctccacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgctactgcg ggcgtaaaca cgcataaagg cagcattttt
     4861 tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca gccggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg
     4981 cgtaccaata attcacaact gacggcaggt caacggttgt acctgcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc cctgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc
     5161 ctactgatgg cgatcaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat ccttacctgg tttttagcac agattta
//
LOCUS       DAZUGK010000253         5368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZUGK010000253 DAZUGK010000000
VERSION     DAZUGK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.255"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..1231
                     /gene="fes"
                     /locus_tag="ACHY5W_004283"
     CDS             29..1231
                     /gene="fes"
                     /locus_tag="ACHY5W_004283"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFS4449047.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1234..1452
                     /gene="ybdZ"
                     /locus_tag="ACHY5W_004284"
     CDS             1234..1452
                     /gene="ybdZ"
                     /locus_tag="ACHY5W_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFS4449048.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            1449..5330
                     /gene="entF"
                     /locus_tag="ACHY5W_004285"
     CDS             1449..5330
                     /gene="entF"
                     /locus_tag="ACHY5W_004285"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFS4449049.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAMQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDEYGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTSHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAVAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         1164 a         1416 c         1565 g         1223 t
ORIGIN      
        1 aattgcacag taaacatggg gttatggtgt gacggcgtta aaagtaggaa gtgagagctg
       61 gtggcagtcg aaacatggcc cggaatggca gcgtctgaat gacgaaatgt ttgaggtcac
      121 tttctggtgg cgtgatcccc aaggttctga agaatactcg acgataaagc gcgtatggat
      181 ctacatcact ggtgtgaccg atcatcatca gaacagccag ccccagtcga tgcagcgaat
      241 tgcaggtact gatgtctggc agtggacgac acaactcaat gccaactggc gcggcagcta
      301 ctgctttatt cccaccgaac gcgatgacat tttttctgca ccatcccccg atcgcctcga
      361 attacgtgaa ggctggcgaa aactactacc ccaggcaata gccgatccgc tgaatccaca
      421 aagctggaaa ggcgggcgag ggcacgctgt ttctgcactc gaaatgccgc aagcgcctct
      481 gcaaccggga tgggattgtc cgcaagcgcc agaaatacca gccaaagaaa ttatctggaa
      541 aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt accaccggcg atgtaacagc
      601 agaagaacgc ccgctggcag ttttgctcga tggcgaattt tgggcgcaaa gtatgcccgt
      661 ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt cctcccgccg tgtatgtgtt
      721 gatcgacgct atcgacacca cgcaccgcgc ccacgaactg ccgtgtaatg cggatttctg
      781 gctggcagta cagcaagagt tattacccct ggtgaaagct attgcccctt ttagcgatcg
      841 cgccgatcgc accgtggtcg ccgggcagag ttttggtggg ctgtccgcgc tgtatgccgg
      901 actgcactgg cctgaacgct ttggctgtgt attaagccag tcaggatcgt actggtggcc
      961 gcatcggggc gggcagcaag agggcgtgtt acttgaaaag ctaaaagctg gtgaagttag
     1021 cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc gagccgatga tcatgcgggc
     1081 caatcaggcg ctgtatgcgc aattgcaccc tataaaagaa tccattttct ggcgtcaggt
     1141 tgacggcgga catgatgcgc tttgttggcg cggtggcttg atgcaggggt taatcgacct
     1201 ctggcaacca cttttccatg acaggagttg aatatggcat tcagtaatcc cttcgatgat
     1261 ccacagggag tgttttacat tttgcgcaat gcgcaggggc aattcagtct gtggccgcaa
     1321 caatgcgcct taccggcagg ctgggacatt gtgtgtcagc cgcagtcaca agagtcctgc
     1381 cagcagtggc ttgaagccca ctggcgtact ctgacaccgg cgaatttcac ccagttgcag
     1441 gaggcacaat gagccagcat ttacctttgg tcgccgcaca gcccggcatc tggatggcag
     1501 aaaaactgtc agaattaccc tccgcctgga gcgtggcgca ttacgttgag ttaaccggag
     1561 aggttgatgc gccattactg gcccgcgcgg tggttgccgg attagcgcaa gcagatacgc
     1621 tgcggatgcg ttttacggaa gataacggcg aagtctggca gtgggtcgat gatgcgctga
     1681 cgttcgaact gccagaaatt atcgacctgc gaaccaatat tgatccgcac ggtactgcgc
     1741 aggcattaat gcaggcggat ttgcaacaag atttgcgcgt cgatagcggt aaaccactgg
     1801 tctttcacca gctgatacag gtggcagata accgctggta ctggtatcag cgttatcacc
     1861 atttactggt cgatggcttc agttttccgg ccattacccg ccagatcgcc aacatttact
     1921 gcgcattgct gcgtggcgaa ccaacgcctg cttcgccgtt tacgcctttc gctgatgtag
     1981 tggaagagta ccagcaatac cgcgaaagcg aagcctggca gcgtgatgcg gcattctggg
     2041 tggaacagcg tcgccaactg ccaccgcccg cgtcactttc tccggcacct ttaccggggc
     2101 gcagcgcctc ggcagatatt ctgcgcctga aactggaatt taccgacggg gaattccgcc
     2161 agctggctat gcaactttca ggtgtgcagc gtaccgattt agcccttgcg ctggcagcct
     2221 tgtggctggg gcgattgtgc aatcgcatgg actacgccgc cggatttatc tttatgcgtc
     2281 gactgggctc ggcggcgctg acggctaccg gacccgtgct caacgttttg ccgttgggta
     2341 ttcatattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggcg gctcaactga
     2401 aaaaaatgcg tcgccatcaa cgttacgatg ccgaacaaat tgtccgtgac agcgggcgag
     2461 cggcaggtga tgaaccgctg tttggtccgg tactcaatat caaggtattt gattaccaac
     2521 tggatattcc tgatgttcag gcgcaaaccc ataccctggc aaccggtccg gttaatgacc
     2581 ttgagctggc cctgttcccg gatgaatacg gtgatttgag tattgagatc ctcgccaata
     2641 aacagcgtta cgctgagcca acgttaatcc agcatgctga acgcctgaaa atgctgatcg
     2701 cccaattcgc cgcggatccg gcgctgttgt gcggcgatgt cgatattatg ctgccaggtg
     2761 agtatgcgca gctggcgcag atcaatgcca ctcaggttga gattccagaa accacgctta
     2821 gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc tccggcgctg gcagatgcgc
     2881 gttaccagtt cagctatcgg gaaatgcgcg agcaggtggt ggcgctggcg aatctgctgc
     2941 gtgagcgcgg cgttaaacct ggcgacagcg tggcggtggc attaccgcgc tcggtctttt
     3001 tgaccctggc gctacatgcg atagttgaag ctggcgcggc ctggctaccg ctggataccg
     3061 gttatccgga cgatcgcctg aaaatgatgc tggaagatgc gcgtccgtcg ctgttaatca
     3121 ccaccgacga tcaactgccg cgctttgccg atgttccaga tttaaccaac ctttgctata
     3181 acgccccgct tacaccgcag ggcagtgcgc cgctgcaact ttcacaaccg catcacacgg
     3241 cttatatcat ctttacctct ggctccaccg gcaggccgaa aggggtaatg gtcgggcaga
     3301 cggctatcgt taaccgcctg ttgtggatgc aaaatcatta tccacttaca ggtgaagatg
     3361 tcgttgccca aaaaacgccg tgcagttttg atgtctcggt gtgggagttt ttctggccgt
     3421 ttattgccgg ggctaaacta gtgatggctg aaccggaagc gcaccgcgac ccgctcgcta
     3481 tgcagcaatt ctttgccgaa tatggcgtaa cgacctcgca ctttgtgccg tcgatgctgg
     3541 cggcatttgt tgcatcgctg actccgcaaa ccgctcgcca gaattgcgcg acgttgaaac
     3601 aggttttctg tagtggtgag gccttaccgg ctgatttatg ccgcgaatgg caacagttaa
     3661 ccagcgtgcc gttgcataat ctgtatggtc caacggaagc ggcggtggat gtgagctggt
     3721 atccggcttt tggcgaggaa ctggcacagg tgcgcggcag cagtgtgccg attggttatc
     3781 cggtgtggaa tacgggcctg cgcattctcg atgcgatgat gcatccggtg ccgccgggtg
     3841 tggcgggaga tctctatctc accggcattc aactggcgca ggggtatctt ggaagacccg
     3901 atctgaccgc cagccgtttt attgccgatc cttttgcccc aggtgaacgg atgtatcgta
     3961 ccggagacgt tgcccgctgg ctggataacg gcgcggtgga gtacctcggg cgcagtgacg
     4021 atcagctaaa aattcgcggg cagcgtatcg aactgggcga aatcgatcgc gtgatgcagg
     4081 cgctgccgga tgtcgaacaa gccgttaccc acgcctgtgt gattaaccag gcggcagcca
     4141 ccggtggtga tgcgcgtcag ttggtgggct atctggtgtc gcaatcaggt ctgccgttgg
     4201 ataccagcgc attacaggca cagcttcgcg aaacattgcc gccgcatatg gtgccggtcg
     4261 ttctgctgca acttccacag ttacctctta gcgccaacgg caagctggat cgcaaagcct
     4321 taccgttgcc tgaacttaag gcacaaacgc cggggcgtgc gccgaaagcg ggcagtgaaa
     4381 cgattatcgc tgtggcattc gcgtcgttgc tgggttgtga cgtgcaggat gccgatgctg
     4441 atttcttcgc gcttggcggt cattcgctac tggcaatgaa actggcagcg cagttaagtc
     4501 gacagtttgc tcgtcaggtg acgccggggc aggtgatggt cgcgtcaacc gtcgccaaac
     4561 tggcaacgat tattgatggt gaagaagaca gctcccggcg catgggattc gaaaccattc
     4621 tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt ccacccggca tccggttttg
     4681 cctggcagtt cagcgtgctc tcgcgttatc tcgatccact atggtcgatt atcggcattc
     4741 agtcgccgcg ccctcatggc cccatgcaga cagcgacgaa cctggatgaa gtctgcgaag
     4801 cgcatctggc aactttactt gaacaacaac cgcacggccc ttattacctg ctggggtatt
     4861 cccttggcgg tacgctggcg cagggcattg cggcgcgact acgtgcccgt ggcgaacagg
     4921 tggcatttct tggcttgctg gatacctggc cgccagaaac gcaaaactgg caggaaaaag
     4981 aagctaatgg tctggatccg gaagtgctgg cggagattaa ccgcgagcgc gaggccttcc
     5041 tggcggcaca gcagggaagt acttcaacgg agttgtttac caccattgaa ggcaactacg
     5101 ctgatgctgt gcgcctgctg acgactgctc atagcgtacc gtttgacggt aaagcgacgt
     5161 tgtttgttgc tgaacgcacg cttcaggaag gtatgagtcc tgaacgcgcc tggtcgccgt
     5221 ggattgcgga gctggatatc tatcgtcagg attgtgcgca tgtggatatt atctctccag
     5281 gggcatttga aaaaattggg ccgattattc gcgcaacgct aaacagataa attaacatta
     5341 tctataaacc cataattgca ggaaataa
//
LOCUS       DAZUGK010000254         5352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZUGK010000254 DAZUGK010000000
VERSION     DAZUGK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.256"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..69
                     /locus_tag="ACHY5W_004286"
     CDS             <1..69
                     /locus_tag="ACHY5W_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449050.1"
                     /translation="EETLLQQPEERFAWLRSKKDRF"
     gene            141..1499
                     /gene="mltD"
                     /locus_tag="ACHY5W_004287"
     CDS             141..1499
                     /gene="mltD"
                     /locus_tag="ACHY5W_004287"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFS4449051.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1547..2170)
                     /gene="yafE"
                     /locus_tag="ACHY5W_004288"
     CDS             complement(1547..2170)
                     /gene="yafE"
                     /locus_tag="ACHY5W_004288"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4449052.1"
                     /translation="MSGLPQGRPTFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWGAR
                     MRTPEALVDAIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2174..2974)
                     /gene="yafD"
                     /locus_tag="ACHY5W_004289"
     CDS             complement(2174..2974)
                     /gene="yafD"
                     /locus_tag="ACHY5W_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4449053.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3215..4129
                     /gene="yafC"
                     /locus_tag="ACHY5W_004290"
     CDS             3215..4129
                     /gene="yafC"
                     /locus_tag="ACHY5W_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFS4449054.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4126..4929)
                     /gene="dkgB"
                     /locus_tag="ACHY5W_004291"
     CDS             complement(4126..4929)
                     /gene="dkgB"
                     /locus_tag="ACHY5W_004291"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFS4449055.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLQESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5092..5168)
                     /locus_tag="ACHY5W_004292"
     tRNA            complement(5092..5168)
                     /locus_tag="ACHY5W_004292"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5132..5134),aa:Asp,seq:gtc)
BASE COUNT         1400 a         1294 c         1324 g         1334 t
ORIGIN      
        1 gaagaaacat tattgcaaca acctgaagag cgttttgcat ggttaaggtc aaagaaagat
       61 aggttctgat aaaactttct tgtcatcggc ttcgttcgcc gttatgatcg gtcgtctttt
      121 aagcaactat tgacacacac atgaaggcaa aagcgatatt actcgcctct gtcctgctcg
      181 tgggttgcca gagtaccggc aacgttcaac agcacgcaca gagcctttct gcagctggtc
      241 aaggggaagc agcaaagttt acaagtcagg cacgatggat ggacgatggg acgtctatcg
      301 cgccagatgg tgacttgtgg gctttcattg gcgacgagct aaagatggga attccggaaa
      361 atgaccggat tcgcgaacag aaacagaaat atttacgcaa taagagctat ctccacgatg
      421 taactttacg ggcagagccg tatatgtact ggatagccgg gcaagttaaa aaacgtaaca
      481 tgcctatgga actggtacta ctacccatag tggagagcgc ttttgatcct cacgcaacgt
      541 ctggtgccaa tgccgcaggc atctggcaga tcattccgag cacggggcgc aattatggtt
      601 tgaaacagac ccgcaattat gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc
      661 tgaacatgat gcagcgtctg aacaaaatgt ttgacggcga ctggcttctg accgtagcgg
      721 cttataacag cggcgaaggt cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga
      781 aatccacgga cttctggtcg ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa
      841 tgctggcatt gagtgatatt ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga
      901 ccgatgaaag ccgtgctctg gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg
      961 ttgcagatat ggcggggatt tccgtcagca agctgaagac attcaacgct ggcgtgaaag
     1021 gctccacgct gggcgcaagt ggtccgcagt acgtgatggt gccaaagaag catgcagatc
     1081 aactgcgtga atctctggct tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg
     1141 acaatacgcc gcttaacagc cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta
     1201 tcgcttcacg tctcggcgta agcaccaaag atttgcagca gtggaacaaa ctgcgcggat
     1261 ctaagctgaa gccaggccaa agtttgacga ttggcgcagg cagtagcgca cagcgactgg
     1321 caaacaacag cgatagcatt acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg
     1381 ctaaacgcca cggcgtgaac atcaaagatg tgatgcgctg gaacagcgac actgcgaatc
     1441 tgcaaccagg cgataagctg acgttgtttg tgaaaaacaa caacatgcca gattcctgac
     1501 aaatcagata atcaaaaggc accgattccc ccggtgcctt ttttatttat gccgctttat
     1561 gtgcatctac catgatgata tcactggtga aagagccatc attctgcaag gcaaaatacg
     1621 ttctcacctc tgtcgatgcg ctctgttggt aaatgcgaat agcgtctact aacgcttctg
     1681 gcgtacgcat tctcgcgccc catgaagaaa attccagcgg taacttatct gtaattaaat
     1741 tatcaactat cagattggct tcattgatta acgtcaacca ctcaccgctg gcgtagtttc
     1801 gtacgtgaga ggtatcgcgt aatgcttcta ccgtctgtaa ccagatgtcg cgcactgggt
     1861 gacccggaga cattacgtcc atcacaatca gcctaccgcc aggtttcaat atcctattca
     1921 cttctcgcag tgctgcacca acatcatgcc agtgatgggc agaataacgg ctgataacaa
     1981 tatcaaatgc gttatcggca aatggcagac tttcggcata tccctggcgg gtggtgatat
     2041 ttttcagttg ccgggcttcg gcagcttgtg ccacgacatc cagcatatgg gcagataagt
     2101 catacgccac caccgtgctc acgttttgcg cagcgacaaa cgttggtctg ccctgtggca
     2161 gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt agcggattgt
     2221 gatcggaagc gcgcgtaacc agtacagaag cttcactgac gttcagacca cggtagaaaa
     2281 caaaatcgag cgggcgacca aacgcccgac ggcgctgatc atcggtaaaa cgcacctgac
     2341 gcagcgacat ttcccgcgca aaacgatata acgcgttcat ccttctacgg ctccaggcat
     2401 tgaaatctcc cgccataatg accgggccgc tgtggtgagc tatctgatca ccaataggaa
     2461 gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt atattaacca
     2521 ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac ttcgccagac
     2581 gcaaaatggg ctctcgttca cgtaacgggc agcaatacac tggatgcgcc gccgaaaggg
     2641 tcattacgcc agaaggatgt tgtggcagca cgaaagcggg tacctgatcg gcggcaagat
     2701 agttagcggt cgcaaactgt actaactctg gcgttgtctg cgcttcctgc aataacacca
     2761 gatgtgcatc tttgccgtag ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt
     2821 atatgttcca caccaggatc cgaatacgct cttcggtact taacggttct ccaggtggta
     2881 atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctttccgca ggttgtccgg
     2941 caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc tctattacgt
     3001 caacccttcc gcactcggga gggttctact gttataggga ttttagctca ctctttcaac
     3061 gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaattt tgctcattat
     3121 tgagaacatt aaatcggatg acatatcacg catcctgatg gtttaatgac aaataaattc
     3181 aaaatatctc ttctattttg caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc
     3241 catttttgtt tcggtcgtag aaagcggcag ctttagccgg gcagcggaac aattagggca
     3301 agcaaactca gcggtaagcc gggcggtgaa aaagctggag atgaaacttg gcgttagcct
     3361 gcttaatcgg accacgcgac aacttagcct gacggaagaa ggcgagcgtt atttccgtcg
     3421 cgtacagtca attttgcagg agatggcagc ggcagaatca gaaattatgg agacgcgtaa
     3481 tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc actttctgat
     3541 gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttgtcgc tagtctcctc
     3601 cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt
     3661 aacggattcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc
     3721 ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa agcaacatgt
     3781 ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagcct gtagcgatgg
     3841 acaattacat gaggtgaagt acggtttgtc atccaatagt ggggaaacac tgaaacagct
     3901 ttgcctgagt gggaacggga ttgcgtgttt gtccgactat atgatcgaca gagaaatcgc
     3961 tcgcggagaa ttggtggagt taatggcaga taaagtgttg ccagtggaaa tgccattcag
     4021 tgcagtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta tcgatttcct
     4081 tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat cccattcagg
     4141 agccagacct tccgggctaa ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt
     4201 atcttcggca tcaagctgta aattttgtgc cttaagatta ctttccaggt ttttacgttt
     4261 agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa tcacttgtgc
     4321 cggagtcgca ttgtgtttag ctgcgatacg agcaataacc tcatctttca gggccttacc
     4381 atacgccagc gtcatatagg aagtaatatg gatgccgtgc tgtttagccc aggcaaccac
     4441 tttacggttt tgcagataag gagagagttc aatctggtta gtagcgatgt tttcagcgcc
     4501 aacagcagca atcgcctttt ccatcaatgg gatcgtgaag ttggaaatac cgatctcacg
     4561 cgtcagccct tgttttttgg cttccagcag cgcctgcata aactcttcaa cagagacttc
     4621 atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac gcaatttttg
     4681 caggctctct tgcagactcg ggatcaattt gtctttgctg agattttcaa tccagatttt
     4741 agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct gacctacggc
     4801 agcttcgtta tcatagattt gtgcggtatc aattgcgcga taaccaagtt ctagcgccgt
     4861 tttcactgat gaaataacaa cgtcgtcttt cagacggaaa gtacctaaac caaatgcagg
     4921 gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc
     4981 agactgttga ggaaaaagag cgtaaaatgc agaggatttt tgcgattctg gcaataatag
     5041 atatacagac gaaaaaaaac ctcaagcgtt aactcgaggc ttcttaataa gtggcggaac
     5101 ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac cgactgaact
     5161 accgctccac cgaattcttt tacaactacc ggttttatga ccggcttact gcttaatttg
     5221 atgcctggca gtttatgaat cacttcgtga ttcacccttc gggccgttgc ttcgcaacgt
     5281 tcaaaagctt tcgcttttgt cctactctct atcgagtagt gaactgcctc aagggtcatt
     5341 gccattcttc gt
//
LOCUS       DAZUGK010000255         5222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZUGK010000255 DAZUGK010000000
VERSION     DAZUGK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.257"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            175..810
                     /locus_tag="ACHY5W_004293"
     CDS             175..810
                     /locus_tag="ACHY5W_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS4449056.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            807..1154
                     /locus_tag="ACHY5W_004294"
     CDS             807..1154
                     /locus_tag="ACHY5W_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFS4449057.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            1159..2067
                     /locus_tag="ACHY5W_004295"
     CDS             1159..2067
                     /locus_tag="ACHY5W_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFS4449058.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILTERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSADIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            2060..2590
                     /locus_tag="ACHY5W_004296"
     CDS             2060..2590
                     /locus_tag="ACHY5W_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFS4449059.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            2601..4529
                     /locus_tag="ACHY5W_004297"
     CDS             2601..4529
                     /locus_tag="ACHY5W_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS4449060.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVALTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKTVKAAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLNSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKCSWSYGNNKIITDTGCGTIHLAGCVIEVMGNKGAMTIRVTTPSTSTGGGTT
                     NAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSRVANDKAVGWNWNSGVYDADI
                     SGASTLILHFNMNAGSCPAVQFRVNYKNGGIFYRSARDGYGFEADWSEFYTTTRKPSA
                     GDVGAYTKAESDSRYVRDIRLGTRVVQTMQKGVMYEKSGHVITGLGIVGEVDGDDPAV
                     FRPIQKYINGTWYNVAQV"
     gene            4498..5061
                     /locus_tag="ACHY5W_004298"
     CDS             4498..5061
                     /locus_tag="ACHY5W_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4449061.1"
                     /translation="MAHGITSHRCNLCSILKNITAGNPKTVAQYQLTKNFDVIWLWSE
                     DGKNWYEEVSNFQEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITANRRADD
                     SGKWMFKDGAVIKRIYTADEQHQLAESQKATLLSEAESVILLLERAVRLNMATEEERS
                     RLEAWERYSVLVSRVDPANPEWPEMPQ"
BASE COUNT         1320 a         1339 c         1416 g         1147 t
ORIGIN      
        1 ttttaccaaa gctaaaaaat gctaatactg caaagctaca aaggaaaaaa acgattagcc
       61 cccacacatt agtcacatta tagctgacca ttacgctctc cttgaatgtt gtctggtagt
      121 tctacaaatg aatccagata gcataacttt tatatattgt gcaatctcac atgcatgaac
      181 actctcgcaa atattcagga actcgcgcgc gcactgcgca acatgattcg caccggcatt
      241 atcgtcgaaa ccgaccttaa cgccggtcgc tgccgtgtgc agaccggcgg catgtgcact
      301 gactggcttc agtggctgac ccatcgtgcc ggtcgttcgc gcacatggtg ggcaccttcc
      361 gtgggggagc aggtgctgat tctggccgtg ggcggtgaac tcgacacggc gttcgttctg
      421 ccggggattt attccggcga taaccccgcg ccgtctgcgt cggcggatgc cctgcatatc
      481 cgtttccctg acggggcggt gattgaatat gaacccgaaa ccagtgcact cacggcaagc
      541 ggaattaaaa cggccagcgt gacggcttct gattctgtta ctgccacggt gccggtggtc
      601 atggtgaaag catcaacccg cgtcaccctg gacacaccgg aggtggtctg caccaacagg
      661 ctgattaccg gcacgctgga agtacagaag ggcgggacga tgcgcggcaa cattgaacac
      721 accggcggtg aactctcatc aaacggtaag gtactgcata cccacaaaca ccccggcgac
      781 agcggcggca caaccgggag tcctttatga cagcgcgtta tctcggaatg aatcgcagta
      841 atggcctgac tgtcactgac cttgagcata tcagccagag tatcggcgat atcctgcgca
      901 cgccggtcgg ctcacgggtg atgcgtcgtg attacggctc gttgctggca tcaatgattg
      961 accagccgca gaccccggcg cttgagttgc agattaaggt cgcctgttac atggcagtgc
     1021 tgaaatggga accccgcgtc accctgtcat ccgtcaccac ggcgcgcagc tttgacgggc
     1081 gaatgacggt cacgttgacc ggccagcaca acgacaccgg ccagccactt tcgttaacca
     1141 tccctgtgag ttgaaaccat gccgattatc gacctgaacc agctacccgc accggatgtg
     1201 gtcgaggagc tggactttga aaccattctc accgaacgca aggcgacact gatttccctt
     1261 tacccggaag accagcagga ggcggtcgcc cgtaccctga cgctggaatc cgagcctctc
     1321 gtcaaactgc tggaggaaaa tgcttatcgt gagcttatct ggcgtcagcg tgtgaatgag
     1381 gctgcacggg cggtgatgct ggcctgtgca gccggtaatg accttgatgt gattggtgcc
     1441 aattacaaca ccacgcgcct gactatcacc ccggcagatg attcgactat cccgccgaca
     1501 ccggcagtga tggaatctga caccgattat cgtctgcgta ttcagcaggc gtttgaaggt
     1561 ttaagcgtcg ccgggtcggt gggtgcgtat cagtatcatg gccgcagtgc cgacgggcgt
     1621 gtcgcggata tctctgtcac cagtccgtct ccggcctgcg tcactatctc tgtgctgtca
     1681 cgtgaaaata acggtgtggc atccgaagac ctgctggccg tggtgcgtaa cgcccttaat
     1741 ggcgaggacg tcaggccggt ggccgaccgc gtgaccgtgc agtctgccga catcgttgaa
     1801 taccagataa acgccacgct gtatctttac cctggtcccg aaagcgaacc cattcgcgct
     1861 gccgccgtga aaaaactgga agcgtatatc acggcacagc accggctggg gcgcgacatc
     1921 cgtctgtctg ccatttatgc cgctttgcat gtggaaggcg tgcagcgtgt cgaactgact
     1981 gcaccgctgg ccgacatcgt gctcaacagt acgcaggcgt ctttctgtac cgaataccgc
     2041 gtcgtgaccg gaggctcgga tgagtgattc gcgactgctg ccgaccggct catcaccgct
     2101 tgaagttgct gccgcaaaag cctgtgcgga aattgaaaaa acgccggtca ggattcgtga
     2161 actgtggaac ccggatacct gtccggcaaa tctgctgccg tggctggcgt gggcgttttc
     2221 ggtcgacagg tgggatgaaa agtggccgga agcgacaaaa cgcgccgtta tccgcgatgc
     2281 ctatttcatc cactgtcata aaggcactat cggcgcaatc cggcgtgtgg tggagccgct
     2341 cggctatctc atcaacgtga cggagtggtg ggaaaacagt gacccgcccg gcaccttccg
     2401 gcttgatatt ggtgtactgg aaagcggcat cacagaggca atgtatcagg aaatggaacg
     2461 gctgattgct gatgccaaac ctgcaagccg ccatcttatt ggcctgaaca ttacccggga
     2521 cattcccggc tacctgttcg ccggtggtgt ggcttacgac ggcgatgtaa ttacggttta
     2581 ccccggataa gtgaggaata atgagcacaa aattcaaaac cgttatcacc actgccggtg
     2641 cagcaaagct ggcagcggca accgcgccgg gagggcggaa ggtcaacatt accacgatgg
     2701 ccgtcgggga tggcggtggt aaattgcctg tcccggatgc cggacagacc gggcttatcc
     2761 atgaagtctg gcgacatgcg ctgaacaaaa tcagccagga caaacgaaac agtaattata
     2821 ttatcgcaga gctggttatt ccgccggagg tgggcggttt ctggatgcgt gagcttggcc
     2881 tgtacgatga tgcgggaacg ttaattgccg tggcgaacat ggccgaaagt tataagccag
     2941 ctcttgccga aggctcagga cgttcgcaga cctgccgtat ggtcatcatc gtcagcagtg
     3001 tggcctcagt ggcgctgacc attgacacca caacggtgat ggcaacgcag gattatgttg
     3061 atgacaaaat tgcagagcac gaacagtcac gacgtcaccc ggacgcctcg ctgaccgcaa
     3121 aaggttttac tcagttaagc agcgcgacca acagcacgtc tgaaacactg gccgcaacgc
     3181 cgaaagcggt aaaggccgcc tatgaccttg ctaacgggaa atacactgcg caggatgcca
     3241 ccacagcgcg aaaaggcctt gtccagctca gtagcgccac caacagcacg tctgaaacgc
     3301 tcgccgcaac accaaaaacg gtaaaggcag catatgacct tgctaacggg aaatacactg
     3361 cacaggacgc caccacagcg cgaaaaggtc ttgtccagct cagtagcgcc accaacagcg
     3421 attctgaaac gcttgcggca acgccaaagg cggttaagac agcgtatgac cttgctaacg
     3481 ggaaatacac tgcacaggac gccaccacgg cgcggaaagg tcttgttcag ctcaatagtg
     3541 ccatcaacag cgattctgaa acgctggccg caacaccaaa agcagtgaag tctgcctatg
     3601 acaatgctga aaaacgtctt cagaaagatc agaacggtgc ggatattccg ggaaaggata
     3661 ccttcacgaa aaatatcggt gcctgtcgtg cttatagcgg cgctttgagc actgaagccg
     3721 gaaactggac aaccgctcag tttattgaat ggctggattc ccgtggtgca tttaatcatc
     3781 cgtactggat gtgcaaatgt tcatggtcat acggcaataa taaaattatt accgatactg
     3841 gctgtggaac tattcatctt gcaggttgcg ttattgaggt tatgggtaat aaaggtgcca
     3901 tgaccatccg tgtaacaaca ccaagcactt ctaccggagg cggcaccact aacgctcaat
     3961 tcacttatat taatcatggt gatgcttatg cccccggctg gcgacgagac tacaacacga
     4021 aaaaccagca gcctgcattt gctttagggc aaacaggaag cagggttgca aatgataaag
     4081 ctgttggctg gaactggaat agcggtgttt atgatgcaga tatcagcggt gcatcgacat
     4141 taatcctcca cttcaatatg aatgcgggga gttgccctgc cgtacagttc cgtgtgaatt
     4201 ataagaacgg cggtattttt tatcgttcag cacgtgatgg ttatggattt gaggctgact
     4261 ggtcagagtt ttacaccaca acccgcaaac cctctgcggg agatgttggt gcatatacca
     4321 aagctgaatc ggattctcgc tatgtacgag atattcgcct gggcacacgt gttgttcaga
     4381 ctatgcaaaa aggtgtgatg tatgaaaaat caggccatgt aattacaggg cttggtattg
     4441 tcggtgaagt cgatggtgat gaccccgcag tattcagacc aatacaaaaa tacatcaatg
     4501 gcacatggta taacgtcgca caggtgtaat ttatgcagca tattaaaaaa tattactgcg
     4561 ggtaatccca aaactgttgc tcaatatcaa ctgacaaaaa attttgatgt tatctggtta
     4621 tggtctgaag acggaaaaaa ctggtatgag gaagtaagta attttcagga agacacgata
     4681 aagattgttt acgacgagaa taatataatt gtcggcatca ccagagatgc ttcaacgctt
     4741 aaccctgaag gttttagcgt tgtcgaggtt cctgatatta ccgccaaccg acgtgctgat
     4801 gactcaggta aatggatgtt taaggatggt gccgtgatta agcggattta tacggcagac
     4861 gaacagcatc aactggcaga atcacaaaag gcaactttac tttccgaagc tgaatccgtg
     4921 attttgctgc tggagcgcgc tgtcaggctg aatatggcaa cagaggagga acgcagccga
     4981 ctggaagcat gggaacgcta cagtgttctg gtcagtcgtg tggatcctgc aaatcctgaa
     5041 tggccggaaa tgccgcagta agttgtatga gctctggtgg gagcttacat atctatggca
     5101 cagagtaaag tctaatctga cagtccgctc tgtgccaaga gcggaagttt tccagcaaat
     5161 tgtcttggtc aaaagagagg cagaccagtt agccccatat ttttcacgct agtcatttct
     5221 at
//
LOCUS       DAZUGK010000256         5180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZUGK010000256 DAZUGK010000000
VERSION     DAZUGK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.258"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1198)
                     /locus_tag="ACHY5W_004299"
     CDS             complement(<1..1198)
                     /locus_tag="ACHY5W_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFS4449062.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIR"
     gene            complement(1439..2485)
                     /locus_tag="ACHY5W_004300"
     CDS             complement(1439..2485)
                     /locus_tag="ACHY5W_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFS4449063.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(2589..2738)
                     /locus_tag="ACHY5W_004301"
     CDS             complement(2589..2738)
                     /locus_tag="ACHY5W_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449064.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            3161..3352
                     /locus_tag="ACHY5W_004302"
     CDS             3161..3352
                     /locus_tag="ACHY5W_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS4449065.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(3405..3638)
                     /locus_tag="ACHY5W_004303"
     CDS             complement(3405..3638)
                     /locus_tag="ACHY5W_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449066.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(3734..4357)
                     /locus_tag="ACHY5W_004304"
     CDS             complement(3734..4357)
                     /locus_tag="ACHY5W_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4449067.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(4446..4955)
                     /locus_tag="ACHY5W_004305"
     CDS             complement(4446..4955)
                     /locus_tag="ACHY5W_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449068.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         1464 a         1299 c         1076 g         1341 t
ORIGIN      
        1 cccggatatt tttttcataa taagaaagct gaagagaagt aaatagcgcc agccttctct
       61 ccggaatcat caatggtttg actttcagta tcatgaagtt taacaacaaa tcaattctgt
      121 ttctgaatcc aatccgcctg tcagaaaggt aatgaatata agccatcagg tcggttgttt
      181 tttgtgagga aaatcggtat ttgtgagaaa aaagctcctc cggtcgaaga ttctgactct
      241 gccattgctg caggtattta tactcaatat tcagctcatt aatttctgct tttaacgaag
      301 cgacctggtt aatggcattc aggtgttttt tcacttgttg aagtacatca tcaatcgcat
      361 ttgcagacgg tgagtcaggt aagacttcct cagaacgcga aatcgacgtt gaaaaaaact
      421 gttgcctgtt ctccgtactc cccagtcgtg ccatcacata tcccagttgc tgtttgtcca
      481 tcttttcata aacattactg acagctgagt tattattgga caaaattgcc actgttttat
      541 tctgaattaa tatattagca acaatattga gtatagtctg tgttttacct gttccaggtg
      601 gtccttcaat aactgaaatc tgtgaggaaa atgcccgttc gacagccagc aactgacttt
      661 cattaagacc aaacggaaaa atcaaacccc atggcgaatc acgtttttta ctctgtcctg
      721 aacagtatgc atgcaaggcg gtatccggat gaggaactat tttctttatc tgcctaagga
      781 tattctcatc gatatttctg ttattaccac gggcataaaa aatacgttca ttagccaccc
      841 tacacaagta attaaacacg gcatgctctg taaatgctga acactgttga aagtcaaggc
      901 cggtcgtttt catgatgtag ggtcgttcac cctcgcggta ataaacagca gcatatttat
      961 caccatatat aataaccagt tcagcccggg atgttacagc gttccctttc ctttcgagga
     1021 aaacatttcg caaatttaca gttggcgtta tctgacatac agaaagcgga cgagtatatt
     1081 ttctcccgct gcgaaaatga caggtcagca taacctctct gtcgtcattt ccagcccaca
     1141 gcgtccagtc ggtaatttta tgagtcttat tttcaccgtc aacacaaatc gtgaccatat
     1201 tcttactcag aaattatcat ttaacttacg gaaaattgtt aaggcactcc cgatattagc
     1261 tccggaggat tttccacaac tccgggactg ttaacatatt tctgcctgcc atatatacat
     1321 tgaatttagt cataacagtc aataccagaa ttacaggaat gcgatgccga tacgctggtt
     1381 attgaatact ggtagcccct gagcactaat tcatcacgat actctgtatt tactgttttc
     1441 actgcttttt acaaccattc ccccaggtaa aatgctgctc ctccggtgat ggctgtgtca
     1501 gaaactgcca tcccggaata gccacgctga ccgccagcaa tgccgcacag acaccagcca
     1561 caaacggttt cagaaacgtg ggcggggatt tctgaaggga gggtgactgc tccgcctgca
     1621 ctttcccctt gcgattatca aggctcagtg catccagacg caccgcgcca tttgccgcaa
     1681 gcagcccttc ccggttatcc acgcttcccg gcagactcag cgaaaaatcc cctgttcccg
     1741 tctgaatcag actgccttta cgatttgaca ggctttgtgc cgatatctgc agtgtgtcgg
     1801 catttatcgt accgccatta ctgctgaact gcccggcagt ccgggcggaa agcgtatcgg
     1861 cactcagttg cgcattctgt gtagacagat ttccactggt tgcattcagc tcagtatgtt
     1921 gtccctgtat ccggctgtta cctaaatcca gcccctgctc aatgtcccgt aaatccagca
     1981 gagaaccttc gtgtgccggg aatgaggaat acaactgaat atagtcatca ctgtccggtg
     2041 tcagacgcac atgtcgcaca acaccgggaa taataaccag ccggaggata ccgcttttta
     2101 aatcctgcga aggtgccaga acacgggtgg tgacataacc atgatcgacc agacggttct
     2161 gcaatgtact catcagcaga ttaattcctt tcgcccccag gcaatgcccg accgccccgt
     2221 tggcgatttt ttgtaaaggc agccagtgtg gtaacgcatc agcccccttc agttccgtct
     2281 gtttaatctg aaaacagggc gtttcaacag gaaaattaat cttatgggca aaagagccag
     2341 gtgcagaaag gcgaacatca ggggctgaag gcgtcagttg ttgctcaagg gcttcctgac
     2401 gctgctgctg aatgataaat gcttcatctc cgctacgccg gacatccgct gcctgaagag
     2461 ataaaggcga taataaagca gacatcagtg tcagtgacag caaggacgga cagcgccgga
     2521 ataagctttg ttcctcagtc ctctcgttcc tgcaacataa ttttccagtc aaacgaaaaa
     2581 ccttttattc atgtcatacc aacaatcacc agcctcctga cgatacctcc caccatgttt
     2641 tgcgatggca cgctaccgcc cttccggtat ttacccgcat caaccggtac cagaatgctg
     2701 agtaacaaac cagctagaat tgacttaatt ttgatcatta tagaataatg atctgaacta
     2761 cagcaacatg acagatttat cgatcgatat aacacaaatg aacagttctt tattctgaca
     2821 aatatcgcaa aaaacagcac attacaaaat ttataaccat aattactgga aaagtgcaga
     2881 aaattattcc actgaatgct tttaatacag ttggttagct taaaaaatag caatttttga
     2941 tttcagtcat tgaaagttgc aatgtttgac gattcaacag catttaagtg agtttcagga
     3001 tatgggaagg acatgaaagg aggagcgcaa taaaatacac aggaagaagg ggaaaatgtt
     3061 tcgtaaaaac agaaaattaa cagcgcacat caggaaacgc agtctcctga ttcagtgcta
     3121 ccacatgaaa atttagttaa ctgttaccgt actaaaattt atgtcagaag atgttccatt
     3181 accgaaagtc aatcagcgat acaaagacga tcatggtgcg cttgtcaccc tgaccagtgt
     3241 tgaggaaacc cgggtggtgt ttatgcagga cggctatccg catccctgca tgcgaccgat
     3301 gtacaacttt ctgggaaaat tcaaacccga accccgggaa gaaacagaat aaaacgaaat
     3361 ccctgccggc aatcacacta cctgatagca gcataatcca cttattaatt ctcatccccg
     3421 gagacaagcc cagaaaacca ctccgggtgt tcactgtatg ccgtgttcag cgcatccctg
     3481 gaaacgttta tataaaagct gtcctgcaat ttgcgggtaa caacttctgc caggcgattc
     3541 cggttatcca ccagtccggc ataacctctc tctgccagcc actcattcag ttcatgttcc
     3601 agagacagat tgataatatc ataatcggac cttgccattc ccgtttcctt agcacatgtt
     3661 ttcaggcacg gggataaagt ataaagaatg tcatactgct gctgccagag caatattccc
     3721 cctgatggtt gtatcagaga tggtatgccg gtactttgcg ggttgttccc gtcaggttat
     3781 cgtgcacctc cacggtcgtg gtcaccaccg gcattccggc ctttctcagc ctcagaacat
     3841 cagccgcaat acgctgactg ccaaaccaga acaggccgtc cagtgcagtc accagtaaac
     3901 ccgcctccag tgcatacttc agccgttcac ggggcgcttt cacttcccgg gcaacctgct
     3961 gatatggcaa tgatgtgttt tcattcccaa ccacgcgacg aatacggtga gacagatgat
     4021 accggtatgt atccggcaca ccagaaaggc tggccttcag gctgtacacg cagccaaacc
     4081 gtttatcgtt aaacaccaca tttttctggc tgatgcccca ttcttcacgc agcgcggcaa
     4141 tcaattgcgg tgtacgggta agcagcaagc gaaaaggcag ttcaaaactg gtgacgtaat
     4201 ccacattcag cagtgcaata cgcaaccgtt cttctggccc ggcctctgtc tgccggcact
     4261 cctccagaac atcctgccac tgccggcgaa gacgggggga ttcgttccgt tctgtaaaac
     4321 agtatttatc cgccagatag tcaattcgtg tacgcatact gaaaggaatt ccgtataaag
     4381 attcagcaga caaaacttta tcagccagta aaaattaacg gaagcattga tattcctccc
     4441 gacaatcacc ggatgattgt tgcaataacc tgcgacataa aggactgaac agccgttttt
     4501 aacgccacgt attgccctga tgaaccaggc cgggccaccc gaaaacatct ttccacccgg
     4561 cattgtccgc cagcacttat caccgaccag gtctgttgca gtaaatccac cagttgcgca
     4621 cattcttcat cagccatgac gccgactcgc ggatggcaaa tactcgtctg gctgagcagc
     4681 aacagacatc gtatctgtgc tccttcatgt tgttcccgca accagcgtaa taccaccggc
     4741 gctgatgggc tgaccgtgtg attatgctcc gtaatacgtt cgtgtacccg tcggtgaaag
     4801 gaactacaaa atgtctgaat ctgttgacca tagattcctt ctgccgaata gagcgtgaag
     4861 tgaaatgcca acagatttgg ccagtggtcc accgcctgct gaacaaaacg ccggatttcc
     4921 cccggctctg aaattaaggc ttcggttatt tgcacgaaat gttccttttc aacccctgtt
     4981 gaattttgtc aagccggtgc agacacagta aagcgcgcat caacacctga aaggtacgat
     5041 acaaacacaa gttacaaaca ataagaacgg aaggacaatt aatcagtgag gaataataac
     5101 taaattaacc aaaagaatgt tactgacaat actaccaata cttaaatagc aatatattaa
     5161 ttaatgtaag aacagatgcc
//
LOCUS       DAZUGK010000257         5152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZUGK010000257 DAZUGK010000000
VERSION     DAZUGK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.259"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1257
                     /gene="garP"
                     /locus_tag="ACHY5W_004306"
     CDS             <1..1257
                     /gene="garP"
                     /locus_tag="ACHY5W_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFS4449069.1"
                     /translation="TAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQ
                     IPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAP
                     SFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGG
                     IGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIK
                     QLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAG
                     GVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
                     KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVF
                     VGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1273..2043
                     /gene="garL"
                     /locus_tag="ACHY5W_004307"
     CDS             1273..2043
                     /gene="garL"
                     /locus_tag="ACHY5W_004307"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFS4449070.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLAWGATFVAVGSDLGVFRSATQKLADAFKK"
     gene            2073..2963
                     /gene="garR"
                     /locus_tag="ACHY5W_004308"
     CDS             2073..2963
                     /gene="garR"
                     /locus_tag="ACHY5W_004308"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS4449071.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3060..4205
                     /gene="garK"
                     /locus_tag="ACHY5W_004309"
     CDS             3060..4205
                     /gene="garK"
                     /locus_tag="ACHY5W_004309"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFS4449072.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGVGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG"
     gene            4238..4614
                     /gene="rnpB"
                     /locus_tag="ACHY5W_004310"
     ncRNA           4238..4614
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHY5W_004310"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4998..>5152)
                     /locus_tag="ACHY5W_004311"
     CDS             complement(4998..>5152)
                     /locus_tag="ACHY5W_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449073.1"
                     /translation="LSDNTLEKDENSPDNCFEVVKYNTYEAYNSEKQYFTREDYTYDY
                     DLLNAI"
BASE COUNT         1195 a         1227 c         1475 g         1255 t
ORIGIN      
        1 accgccgtta actacgccga tcgtgcaacg ctgtctattg ctggtaccga agtggcaaaa
       61 gagttgcagt taagtgcggt ttcgatgggt tacatctttt ctgcttttgg ctgggcctac
      121 ttgctgatgc aaatccccgg cggctggctg cttgataagt ttggctcgaa aaaagtttac
      181 acctatagcc tctttttctg gtcgctattc accttcctgc aaggctttgt tgatatgttc
      241 ccgctggcct gggcagggat ctccatgttc tttatgcgct ttatgctggg gttttcggaa
      301 gcgccatcat tcccggcgaa cgcccgtatt gtcgccgcct ggttcccgac gaaagaacgt
      361 ggtactgcct ccgccatctt taactcggcg caatatttct cgctggcgct cttttcgcct
      421 ctgcttggct ggctgacttt cgcctggggc tgggagcacg tctttaccgt tatggggggg
      481 attggttttg tgctgacggc gctgtggata aagttgattc ataacccgac agatcaccca
      541 cgtatgtctg cggaagagct gaagtttatc tctgaaaatg gcgcggtggt cgatatggac
      601 cacaaaaagc cgggcagtgc ggcagcaagc ggacccaaac tgcattacat caagcaattg
      661 ctctctaacc gcatgatgct tggcgtattt ttcggacaat attttatcaa caccatcacc
      721 tggttcttcc tcacctggtt cccgatttat ctggtgcagg aaaaaggcat gtcgattctg
      781 aaagtgggtc tggtcgcctc gattccggca ctgtgtggtt ttgcgggcgg cgtgctggga
      841 ggtgtcttct cggattatct gatcaaacgc ggtttatccc tgaccctggc acgtaagcta
      901 ccgattgtgc tgggaatgtt gctggcttcc accatcatct tatgtaacta caccaacaac
      961 accacgctgg tggtcatgct gatggcgctg gctttctttg gcaaaggatt tggtgcgctg
     1021 ggctggccgg taatttctga caccgcgccg aaagagattg ttggcctctg cggcggcgtc
     1081 tttaacgtct ttggtaatgt tgcctccatt gtcactccac tggtgattgg ctacctggta
     1141 agtgaactgc actccttcaa tgcagcactg gttttcgtgg gatgttcagc gctgatggcg
     1201 atggtctgct acctcttcgt agttggcgac attaaacgta tggaattgca gaaataagca
     1261 aaggtataag cgatgaataa cgatgttttc ccgaataaat tcaaagccgc actggctgcg
     1321 aaacaggtac aaattggttg ctggtcagca ctctctaacc cgattagcac tgaagttctt
     1381 ggtttggctg ggtttgactg gctggtgctg gatggcgaac atgcgccaaa cgatatctcc
     1441 acgtttattc cgcagttaat ggccctgaaa ggcagcgcca gcgcgccagt agtacgagtg
     1501 ccgaccaacg agccggtaat tattaagcgt cttctggata tcggtttcta taacttcctg
     1561 attccttttg tagaaacaaa agaggaagca gagcaggcgg tggcatcgac gcgttatccg
     1621 ccagaaggca ttcgcggcgt ctccgtttct caccgcgcca atatgtttgg caccgtggcg
     1681 gattatttcg ctcagtcgaa caagaacatc actattctgg tccagataga aagtcagcag
     1741 ggcgtagata acgtcgatgc cattgccgct accgaaggcg tagacggcat cttcgtcggc
     1801 cccagcgatc tggctgcggc attaggccat ctcggcaatg cctcgcaccc ggatgtacaa
     1861 aaagcaattc agcacatttt taaccgtgcc agcgcgcacg gcaaacccag cggtatcctc
     1921 gcgccggtcg aagccgatgc gcgtcgttat ctggcatggg gcgcgacgtt tgtggctgtc
     1981 ggcagcgatc tcggcgtctt ccgctctgcc actcagaaac tggctgatgc ctttaaaaaa
     2041 taaccaccac cgcaacaaga gagatgattg atatgactat gaaagttggt tttattggcc
     2101 tggggattat gggtaaacca atgagtaaaa accttctgaa agcaggttac tcgctggtgg
     2161 ttgctgaccg taacccagaa gctattgctg acgtgattgc tgcaggtgca gaaacagcgt
     2221 ctacggctaa agcgatcgct gaacagtgcg acgtcatcat aaccatgctg ccaaactccc
     2281 ctcatgtgaa agaggtggcg ctgggtgaga atggcattat tgaaggcgcg aagccaggta
     2341 cggtattgat cgatatgagt tctatcgcac cgctggcaag ccgtgaaatc agcgaagcgc
     2401 tgaaagcgaa aggcattgat atgctggatg ctccggtgag cggcggtgaa ccgaaagcca
     2461 tcgacggtac gctgtcagtg atggtaggcg gcgacaaggc tattttcgac aaatactatg
     2521 atttgatgaa agcgatggcg ggttccgtgg tgcataccgg ggaaatcggt gcaggtaacg
     2581 tcaccaaact ggcaaatcag gtcattgtgg cgctgaatat tgctgcgatg tcagaagcgt
     2641 taacgctggc aactaaagcg ggcgttaacc ctgacctggt ttatcaggca attcgcggtg
     2701 ggctggcggg cagtaccgtg ctggatgcca aagcgccgat ggtgatggac cgcaacttca
     2761 agccgggctt ccgcattgat ctgcatatta aggatctggc gaatgcgctg gatacttctc
     2821 acggcgtcgg cgcacaactg ccgctcacag ctgcggttat ggagatgatg caggcactgc
     2881 gtgcagatgg tttaggaacg gcggatcata gcgccctggc gtgctactac gaaaaactgg
     2941 cgaaagtcga agttactcgt taatgacgtc gtgcccggat ggcgtattgc aatccgggcc
     3001 tggaatccag gccgaataag agaaacgccc tccggcgtca tgtggtaaca ggcataggta
     3061 tgaaaatcgt aatcgcccca gactcttata aagaaagttt atctgccagc gaggttgcgc
     3121 aggcgataga aaaaggattt cgggaaattt ttcctgatgc acagtacgtt tctgttcctg
     3181 ttgccgacgg tggcgaagga acggtggaag cgatgattgc agccacccag ggttccgaac
     3241 gtcacgcctg ggttacaggg ccgctgggcg agaaagtgaa tgccagttgg gggatctccg
     3301 gcgatggcaa aaccgcgttt attgaaatgg cggcggccag tgggctggag ctggtacctg
     3361 cggaaaaacg cgatccactc gtgaccactt cacgcggcac aggcgagtta atcctgcagg
     3421 cgctggagag cggcgcgaca aacattatta tcggcattgg cggcagcgct acaaatgatg
     3481 gcggcgtagg catggtacag gcgctggggg cgaaattatg cgacgccaac ggcaatgaaa
     3541 ttggttttgg cggcggtagt cttaatactc tgaatgatat tgatatttcc ggcctcgatc
     3601 cgcgcttaaa agattgcgtc attcgcgtcg cttgtgatgt caccaatccg ctggtgggcg
     3661 ataacggcgc atcgcgcatc tttggcccac aaaagggagc cagtgaagcg atgattgttg
     3721 agctggacaa taacctctct cactatgccg aggtcattaa aaaagcactg catgttgatg
     3781 tgaaagatgt ccccggcgca ggtgctgctg gcggtatggg agcggcgctg atggcgtttc
     3841 ttggtgcgga actgaaaagt ggtattgaaa tcgtcactac ggcgctgaat ctggaggaac
     3901 atattcacga ttgtacgctg gtgatcaccg gtgaagggcg tattgacagc cagagtattc
     3961 acggcaaggt accgattggc gtcgcaaacg tggcgaagaa gtaccataaa ccggtgattg
     4021 gcattgcggg tagcctgacc gatgatgttg gcgttgtaca tcagcatggt attgatgcgg
     4081 tcttcagcgt actgaccagc attggtacgt tggacgaagc attccgcggg gcttatgaca
     4141 atatctaccg tgcttcacgt aatatcgccg cgacactggc gattggaatg cgcaacgcgg
     4201 ggtgacaagg gcgcgcaaac cctctatact gcgcgccgaa gctgaccaga cagtcgccgc
     4261 ttcgtcgtcg tcctcttcgg gggagacggg cggaggggag gaaagtccgg gctccatagg
     4321 gcagggtgcc aggtaacgcc tgggggggaa acccacgacc agtgcaacag agagcaaacc
     4381 gccgatggcc cgcgcaagcg ggatcaggta agggtgaaag ggtgcggtaa gagcgcaccg
     4441 cgcggctggt aacagtccgt ggcacggtaa actccacccg gagcaaggcc aaataggggt
     4501 tcataaggta cggcccgtac tgaacccggg taggctgctt gagccagtga gcgattgctg
     4561 gcctagatga atgactgtcc acgacagaac ccggcttatc ggtcagtttc acctgattta
     4621 cgtaaaaacc cgcttcggcg ggtttttgct tttagagggg cagaaagatg aatgactgtc
     4681 cacgacgcta gacccaaaag aaagcggctt atcggtcagt ttcacctgat gtacgtaata
     4741 aaccgttccg gcgggtttca gactgttgag tgcgctttat tcatgccgga tgcggcgtga
     4801 acgccttatc ctgcctacaa aagcacgcaa attcaatata ttgcagagat ggcgtaggcc
     4861 tgataagcgt agcgcatcag gcatttttgc ggttgccatc agtttcaaac ccgcgtcgat
     4921 aagtttttgc ttttggtgca cagggtaaga tgaatatcta cggtattaat aatagacttt
     4981 gtaagaagtt attaagtcta tattgcattt aaaaggtcgt aatcatacgt atagtcctct
     5041 ctggtaaaat attgcttctc tgagttatat gcttcatatg tgttatattt tacaacctca
     5101 aagcaattgt caggagaatt ttcgtctttc tcgagtgtat tatctgaaag ga
//
LOCUS       DAZUGK010000258         5140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZUGK010000258 DAZUGK010000000
VERSION     DAZUGK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.260"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..999
                     /locus_tag="ACHY5W_004312"
     CDS             <1..999
                     /locus_tag="ACHY5W_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4449074.1"
                     /translation="LLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQ
                     GERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            992..1333
                     /locus_tag="ACHY5W_004313"
     CDS             992..1333
                     /locus_tag="ACHY5W_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449075.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            1333..2031
                     /locus_tag="ACHY5W_004314"
     CDS             1333..2031
                     /locus_tag="ACHY5W_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449076.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(2048..2368)
                     /locus_tag="ACHY5W_004315"
     CDS             complement(2048..2368)
                     /locus_tag="ACHY5W_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4449077.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            2476..2649
                     /locus_tag="ACHY5W_004316"
     CDS             2476..2649
                     /locus_tag="ACHY5W_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4449078.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            2720..3643
                     /locus_tag="ACHY5W_004317"
     CDS             2720..3643
                     /locus_tag="ACHY5W_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449079.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHHY"
     gene            3698..4435
                     /locus_tag="ACHY5W_004318"
     CDS             3698..4435
                     /locus_tag="ACHY5W_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4449080.1"
                     /translation="MLYSNILAHARRCAPAESCGFVISTPEGERYLPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SAWRHRHWHASVFTGIYNDLAAASACT"
     gene            4432..5010
                     /locus_tag="ACHY5W_004319"
     CDS             4432..5010
                     /locus_tag="ACHY5W_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4449081.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT         1218 a         1184 c         1558 g         1180 t
ORIGIN      
        1 ctgctgcgta atgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
       61 gaagagacac tgaagaatga gaatgcggct gcgcgtgcgt cgaatgatgc tgaactgctg
      121 gggtacgggc agggggaacg agccagagaa cgcatgcggg agttgcagca gatccgcgac
      181 agcttccgcc agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag
      241 gatttttaca gacaggctct ggcgcagaat gcacagtatc tgagtgaacg tctgaaagag
      301 caggaagcct tttatgccga atcggatgtg cagcgtgcgg actggcagaa agggctgcag
      361 gaggggttca gtaactgggt ggataatgcg tccgattacg cctcacaggc agcacagctt
      421 gcgacggagg gtatctcagg gatggtgaat aacatcacgg agatgctgaa cgggaataaa
      481 gtggaatggc gcagctgggc ctcatcagtg ctgcaggaaa tatcaaaagt tcttatgaat
      541 gccgcgattg tcaacggaat taagacggcg gcaaacggta tgtccggtgc gggaggattt
      601 ctcggcagca ttggtgactg gctgggcggg gcggtggcca atgcaaaagg cggcgtgtat
      661 acctcggcaa acctgagtgc gtacagcaac agtattgtgg atacgcccac gtactttgcc
      721 tttgcaaaag gggccgggct gatgggggag gccggacctg aagccattat gcccctgacc
      781 cgggcggcgg atggctcgct gggtgtgcgc gcggtgggca gtatgaacgg cagtgcgggt
      841 ctggtgtatt ccccggtcta ccatatcgcc attcagaatg acgggactaa tggccagata
      901 gggccggagg cggcaggcag tcttgtgcag ctgattgacc agcgggtgca ggcggtgatg
      961 ctgtccatgc gacgtgacgg aggaatgctg agtggctgag ataaaaacgc tgcatctggt
     1021 cccgcgtgaa gggatgcagg tgagtgagaa gccgtcggtg gtgagggtgc ggtttggtga
     1081 cggttatgaa cagcgccgcc ccacagggct gaatcctcaa ctgaagacgt ttcaggcggt
     1141 gttccgggtg acggatgagt caacccggcg ctggctggaa gagtttttat cgtggcatgg
     1201 tggttaccgt gcctttttgt ggcgaccgcc gaaacataac cggacggtga gggttgtgtg
     1261 ccgggagtgg agtgtcacgg ataatgcccg gtacagtgat ttcagttgca cgatagagca
     1321 ggtggtgaac tgatgcagga tattcacgaa gaaagcctga acgagtcggt taagtcagag
     1381 cagtcaccgc gggtggtact ctgggaaatc gacctgacgg tacagggtgg tgagcggtat
     1441 tttttctgca atgagctgaa tgaaaaaggg gaggcggtca cctggcaggg gcggcaatat
     1501 caggcatacc cgattgacgg cagtggcttt gagatgaacg ggaagggcag cagtgcccgc
     1561 ccgtcgctga cggtgtcgaa tctgttcggt ctggtcaccg gaatggcgga ggacctgcag
     1621 agcctggtgg gggccacggt ggtccgccgc cgggtgtatg cccgttttct ggatgcggtg
     1681 aattttgtgg cgggcaatcc ggaagcggac ccggagcagg agctgagcga ccgctgggtg
     1741 gtggagcaga tgtcagagct gacggccatg acagcctcgt ttgtgctggc cacaccgacg
     1801 gagacggacg gggcgctgtt tcccggtcgc atcatgctgg cgaacacctg tatgtgggat
     1861 taccggggag atgaatgcgg gtataacggt cctgcggtgg cggatgagtt cgacaacccc
     1921 accacggata tccgtaagga cagatgcagc aagtgcatgc gcgggtgtga gatgcgcggc
     1981 atggtggcta attttggcgg tttcctttcc attaataaac tttcgcagta atggattatg
     2041 cccaccgtca ggtgggtttt ttatttagta gttctctcaa cttttcgttc tgctctctga
     2101 acttttcctg tatttctttt tgcatggcga taacctgggc ttgaagttga actagcgcat
     2161 caacatttag cggaaccgag acgctgttaa ttttatctgc tatttccccg agtgtatttt
     2221 ctgcattcaa ggcatcttcc agtatctgaa caatctctga gttcatagag cgcccgtttc
     2281 gtttggctcg ttcagctata gcatctcgca ttccgtcagg aaaacggagg ttgaacttgt
     2341 cgtagtcttt tacttgtttt tcggccattg caaatctctc aaaaaaaatc atggtgccat
     2401 attgccatac gatttcaata gtggcattat ggccttcatg gcgtcacttt ggcaccaaat
     2461 aaaggagatt agataatgca agatacactt ttcactgagc gcaaaaatat caaactcaac
     2521 cttcgccttc catcacggct gaatgaagac cttcgccgcc tggcggaaat ggactgtata
     2581 tctctgaact ctgcaattgt tcgtttgctg gcaaaaggtg ttagggaaga ggtggcgaat
     2641 ggtcgctaaa aacagcgaag cctcaatggc tgcaaccatt gaggcttcta aattaccagt
     2701 taaccacgag aaaactgata tgactagttt agcaattgca gatcgcacaa tcaatgttcc
     2761 attccacgga acaaatctct ttttggttgg aattaacaat gagccttatg ttcctatgaa
     2821 gcctgttgtt gaaggtatgg ggatggtttg ggctgctcaa tttgttaagt taaaacagag
     2881 gtttgtcaag ggtatttcgg aaatcgaaat accttctgct ggcggtaaac agttaatgac
     2941 atgtcttgcc tttcgtaaat ttgcggcttg gctttcaagc attcaaccaa acaaagtccg
     3001 ccctgaaatc cgcgacaagg taatccagta tcaggaagag tgtgacgatg tgctctacga
     3061 gtactggact aaaggccatg tgattaaccc gcgcaaagct aaaaaggcat tgccggggaa
     3121 aatcaccact gaacagcagg aagccattaa acaactcgtc atgagtcgcg gtcagtctct
     3181 gccaaaagaa aaacaggcga aggcgatgat caccatgtgg tcgtcactga aatctcattt
     3241 tgggtgttca tacaaagaaa tcagcgagga gcagtttacc gaagctctgt cacttgctgc
     3301 tcgcgttccg cttgaaggag agttaatcgg caaacaagag aagagcacca acgagctttc
     3361 cgcaaaagaa gcaaacagcc ttgtatggct atgggattat gccaaccgct cacaggcatt
     3421 attccgcgaa ttgtatccag cgctaaagca aattcaatcg aactattccg gcagatgtta
     3481 cgactacggt catgagttct cgtatgttat tggaatggcg agagatgttt taatcaatca
     3541 cacacgagat gttgatatta atgagccaga cggaccaacg aatctttccg catgggtaag
     3601 gcttaagaac aaagaattac ctccttcact acatcactac taacagattg ccaacgaaat
     3661 gacccagctt cggctgggtt ttttatcagg agttctcatg ctctatagca atatattggc
     3721 gcacgcccgg cggtgtgcgc ctgcggagtc gtgcggcttc gtgataagca cgccggaggg
     3781 ggagcggtat ctgccgtgtg tgaatatctc tgcagagccg gaggcgtatt ttcgtattgc
     3841 accggaagac tggctgcggg cagagatgca gggggagatt gtggcactgg tccacagtca
     3901 tcccggtggt ctgccctggc tgagcgaggc cgaccggcgg ctgcagataa aaagtgcact
     3961 gtcctggtgg ctggtctgcc ggggggacat tcataaattc cgctgtgtgc cacatctgac
     4021 aggacggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg atgcctacca
     4081 tctggcggga attgatatgc cggattttga gcgtgaggat gactggtggc gcaacggtca
     4141 gaacctttac ctggacaata tggcggtcac aggcttttac cgggtgcccc tgtcctctgc
     4201 acaggcgggc gatatcctgc tgtgctgctt tggcgcatcg gtggctaatc atgccgccat
     4261 ttactgcggc aacggtgaac tgcttcacca tctgcctgaa caactgagta aacgggagag
     4321 gtattccgaa aaatggcaac gacgaacgca ttcagcctgg cgtcaccgcc actggcacgc
     4381 atctgtcttc acggggattt acaacgattt ggccgccgcc tcagcctgta cgtgaacacg
     4441 gcagcggaag ccatccgtgc cctgtcgctg cagatgccgg gattccgccg tcagatgaac
     4501 gaaggctggt accagatacg tattcgcggt gaggacacgg caccggaggc ggtgtacgcc
     4561 cgtcttcacg aacctctggg tgagggggcg gtcatccata ttgtgccgcg actggccgga
     4621 gccggaaagg gtggactgca gattgtgctg ggggcggcag ccatcgtggg ctctttcttc
     4681 actgccgggg catcaatggc gttatggggt tcagccctgg cagccggtgg tttttctgcc
     4741 accacgatgc tgttttctct gggggccagc atgattctgg gtggtgtggc ccagatgctg
     4801 gccccgaagg caaaaacacc ggattaccgc gcaacggata acggcagaca gaacacgtac
     4861 ttttcgtcac tggacaacat gattgcccag ggtaacccga tgccggtgcc ttatggtgaa
     4921 atgctggttg gttcacgacg gatatcccag gacatcagca cccgtgatga gggcggagac
     4981 gggaaagtgg tggttatcgg gcggggatga aaataaaaaa atcccgcaga gttagcggag
     5041 ctgcgggaga gaacgatgaa gattaacgtt atggagttat ttttcaggca tcaaaaaagt
     5101 aacgcagcgt cattattgcg gctacaggca attgccggaa
//
LOCUS       DAZUGK010000259         5112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZUGK010000259 DAZUGK010000000
VERSION     DAZUGK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.261"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..985
                     /locus_tag="ACHY5W_004320"
     CDS             134..985
                     /locus_tag="ACHY5W_004320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4449082.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1012..2001
                     /locus_tag="ACHY5W_004321"
     CDS             1012..2001
                     /locus_tag="ACHY5W_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4449083.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPAADTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2033..2926)
                     /locus_tag="ACHY5W_004322"
     CDS             complement(2033..2926)
                     /locus_tag="ACHY5W_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449084.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3127..4053)
                     /locus_tag="ACHY5W_004323"
     CDS             complement(3127..4053)
                     /locus_tag="ACHY5W_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449085.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDVLKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            4210..>5112
                     /locus_tag="ACHY5W_004324"
     CDS             4210..>5112
                     /locus_tag="ACHY5W_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4449086.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPF"
BASE COUNT         1395 a         1254 c         1175 g         1288 t
ORIGIN      
        1 aacttgtact actcatctca acactcaaac agatttatga atttcgttca taacccctga
       61 caaatcctgc cgtctaatcg agctcttctc tttacgttat gcttctcatg aacgataacg
      121 gaaggataac accatgcaaa aagtaaaact gaataacggt attgaaatgc ccctgctggg
      181 ctttggtgta tttcagatga ccgatgccgc cgaatgcgaa agagcggtca ttgacgctat
      241 taacaccggt tatcgactga ttgacaccgc cgcctcctat caaaatgaaa cccaggtcgg
      301 gaacgcactg aaacaaagcg gtattgcccg taacgaactc tttgtcacca cgaaactctg
      361 gttgcaggat accagttacg aaggggccaa agcccagttc gaacgctctc tgaatcgact
      421 gcaacttgat tacgttgacc tgtatttgat tcatcagcct tacggtgatg tccacggtgc
      481 ctggcgtgcc atggaagagc tgcaacaggc cggtaaaatt cgcgctattg gcgtcagtaa
      541 cttccatcct gaccgactgg ctgaccttat cgcctttaat aacgtcgttc cagcggtaaa
      601 ccaggttgaa gttaaccctt tcaatcagca gctacacgcc gttccgtgga tgcaaagtcg
      661 tggtattcag ccagaagcct gggcaccgtt tgcggaaggg aaaaatggtc tgttccagca
      721 tcctgttctc acagcaattg gcgagaagta tggcaaaagc gtaggccagg ttgttctgcg
      781 ctggatctac caacgaggca ttgtttcact agcaaaatca gtgcggaaag aacgcatgga
      841 agagaacatc aacattctcg attttgaact cagccctgaa gatatgctgc aaattaccgc
      901 cctggatacc gcaaccagcg cgttcttctc tcaccgcgat cccgcaatgg tggaatggct
      961 gactggccgc aaacttgatg tttaagccgc gataaaagag gtattcattc aatgcaaaaa
     1021 cgttatctgg gtaaatccgg actcgaagtc tccgcccttg ggctcggttg catgggctta
     1081 agccacggct acggcccggc ggccgatacc cgtcaggcta tcgaacttat tcgtgctgcg
     1141 gttgaacgtg gcgtcacctt cttcgatacc gccgaagtgt atggtccgta tctgaatgaa
     1201 gaagtggtcg gcgaagcctt aaaaccgttt cgtgaccgcg tggtgatcgc cactaaattc
     1261 ggttttacct tcggcgatga taataagcag cagattttaa acagccgtcc ggaacatatc
     1321 cgtgaagccg ttgaaggatc attacgtcgt ctgaagacag atgttattga cctgctgtac
     1381 caacaccgtg ttgatccgga tgttcctatt gaagatgttg caggaacggt gaaagacctc
     1441 atcgcagaag gtaaggtcaa acatttcggc ctgtctgaag cgggagcaca aaccattcgt
     1501 cgtgcgcatg cggtacaacc tgtcaccgcc ctgcaaagcg aatattccat gtggtggcgt
     1561 gagcctgaac aggaaattct gccgttgctg gaggaactgg ggattggttt tgtccccttc
     1621 agcccactag gtaaaggttt cctgaccggg tcgattaagc cgggaaccac tttcggcaaa
     1681 gatgattacc gcagcaccgt accgcgtttc gcagcacaag ccattgaagc taatgaaaaa
     1741 ctggtcacct tattaggtga actggctgcg gagaaagggg taacgtctgc acaaatcgca
     1801 ctggcatggc tgttagcaca aaagccgtgg attgttccca tccctggcac cactaaactg
     1861 caccggctgg aagaaaacct ggccgctgct gacattgttc tttcgcagaa agatacgcag
     1921 cagataacag aggcgctgga aacaattaaa atcgtcggtg aacgttactc gcctgaacac
     1981 caggcacgcg taggccgcta atacccaaac gggtccagga acaaaggacc cgttattcgg
     2041 tgtagcgaag cgcatcgatc aacagcgcaa aagcaggcgg atgctgcttg cggctggggt
     2101 agtagagata atatccggga aaagttggac accactcctg caacacctga ataagcttgc
     2161 ctgactttat atactcctga accatgtctt cagggacaca agcgatccca aaccctgata
     2221 acgcggcatc aattctttct ggcaatggac tacacgtcag ctgcccatca accttaacgc
     2281 gaagaggttt cccttctcgc tcgaactccc agtggtaaag tccaccggca gttggcaaac
     2341 gcatgttgat gcagcgatgg ttttgtagct cgtgaggcgt ttcaggcgca ggatttgcag
     2401 caaaatacgc cggtgccccc accacagcca tccgcatatc tggtccaatt ctgaccgcaa
     2461 ccatatcttt atcaacattt tcacccagtc gtatcccggc atcaaaacgg ccttcaacaa
     2521 tatcgacaaa accgttatcc accaccagtt cgacactgat ttcgggatac tttctgagaa
     2581 aaggttttag cttcggccag accagacttc gcgcggcatg ttccccggca gataaacgaa
     2641 tattcccgga gggcgtgccg ttctgttgaa taagtgactc cagttcctgt tcaagctcgt
     2701 caatacgcgg ttcaaggcag gcaatgattc tctcccccgc ttctgtggga gccacactgc
     2761 gggtagtccg tgtcaggagg cggatattga gcctttcctc cagagccttc attgcatggc
     2821 taagtgcaga ctgagagacg ccgagtttgc ccgctgcttt ggtaaaactt cgctctcttg
     2881 ccaccacaag aaagatttgc agttcgttga agttttcttt gagcattggc gatttccttg
     2941 tagaaacatt ctcttcacag acactcatga agggggggca tagatgctat tattttagcc
     3001 acattattag taacaatgat aatctggctg tatcgaaaat aattctaaac tttcaggaga
     3061 tttattatga ataaacgtat cctcacgatt gcgccattct tcttaccgat attctgacac
     3121 acaacattaa ctttctattt ttactctttt ctccaccata aaatcaataa aagcacgaac
     3181 tttagagggc attctcaccc gtgatgaaac ataaatataa aatccaggaa atgaaacagc
     3241 ccaatcctca agaatcggct gtaacttgcc cgcattgata tgttcagcga ttgcaaaatc
     3301 cagatgttga atgatacccg ttccattcag cgccattcgt atcatgcttt cgtcatcatt
     3361 agaaatatac ttacctgaaa cctctatttc gaactcttca ttacccccca caacattaga
     3421 aaactcccat gcagagagtg caccgctact tttatgccgc aaccgaaggc aattatggtt
     3481 actcagttcc tgaggcgttt caggaatacc gtgttctttt aaataatctg gcgatgccac
     3541 agtaaccagt ttaataagcg gagaaacagg aacggcagtc atatgctcat ccagtccttg
     3601 ctcaagacgt atgcccacat cacagccgtc cgcaataatg ttagataaac cgtcatccat
     3661 aatgagttct atctttgttt tggggtaacg agtaagaaac tcgccaatat gaggctcaac
     3721 aagcatccgt gctgcaacac gggaggaatt tattctcaac aggcctgtag gttcaagttg
     3781 ggtatcacca acatctttca aaacatcatc aatttgcccc aaagccgaca ccagcacttc
     3841 atacagacgc tgtccttcct cagtaagcga catattgcgg gtcgtacggt atatcagctt
     3901 ggtgttaaga cgttcctcaa gagattttaa attctgagac aatgcggccc gcgtaatacc
     3961 catttcagaa gcggctctgg aaaaactgcc ggcacgaaca atcagcgcaa accaggcaag
     4021 agaaggcaaa attgaaggct ctattttcat cattgtaaac cttaacttaa cactgtgata
     4081 gatattagac cattgatgat aaccaataca agtcatacta ttacgccgtt ctcgatatga
     4141 acacgaatgc cagttgcgga taatagaaat gatatttaaa tctgtttagg caaatccccg
     4201 gagcttgaaa tgaaaactgt ttcgataaaa cacacttact gggatatagc tgctgatatc
     4261 tatttccctg acgattttaa tagtgaaaaa aagtatcctg ccattatcag cgcccatcca
     4321 attggaagct gcaaagaaca gacatcgggt agtgtgtatg gtgccgccct ggcaaaagct
     4381 ggctttatcg tcattgcttt tgatgccagt tttcagggaa gcagcggtgg ggaaccgcgt
     4441 tatctggaag atccaacaat gcgagtaaaa gacttcagca ttgtcgttga ttaccttacc
     4501 agcttacctt acgttgatgc cgggcgtatt ggtgtactgg gtatttgtgg tggaggtgga
     4561 tatgccatta atgccgcaat gacagaacgt cgaattaaag ctattggtac tgtcacgggt
     4621 gcaaactacg ggcgtcttat gcgtgaggga tttacagcgt ttaatcctat cggagcactc
     4681 gaagcaatgg cgcaacaaag aacagacgaa gctaacggcg caaaacttcg cgtagatgat
     4741 ttactcccct cttctccgga tgcagctctt gaagcgggat taacagaaat agaccttttt
     4801 gaagccacag aatattatcg ttcgccacga ggctgtgcac ctaatggggt aaatcgttcg
     4861 ctattctcac atcagactgt tgccgtgggt tgggatgcat ttcatcttgc agaagtcctg
     4921 ttaactcaac caataatggt tgtggtgggc gatagagtgg gcgcatttgg tgcctaccgt
     4981 gacggctgcg aaattattgg ccgcgctgcc tcaaaacata aagagctggt cgtggttgaa
     5041 ggttattcac attacgacct gtatgataag ccagaacctg tgaaacaggc actcgataaa
     5101 ttaattcctt tt
//
LOCUS       DAZUGK010000260         5075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZUGK010000260 DAZUGK010000000
VERSION     DAZUGK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.262"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1315)
                     /locus_tag="ACHY5W_004325"
     CDS             complement(<1..1315)
                     /locus_tag="ACHY5W_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449087.1"
                     /translation="MVTPCHIKASAGMTLPLALENQESCRTRLEEMINLINRNTDCST
                     GVPDQVNALVATMADALQPYTAMTNELPEYPNMPGRILYIPEPFSFCFSGHTITFTNT
                     KRDYNGDNLVNIAVSKDGGNARGDADCALFHAFVTTMLSHSRQPGTYDFFPSLKEEKQ
                     GEGMHFMVTPRNSSISTDAIMSQPLLQSQHNWGILNKIINLINADTDEVNELVVAMTN
                     ALFLYQTVVRSELQSTEPFTEPFRFSFFFSSHTITFTKAESNSKLVQIEVSRGGVTIR
                     ECIGSARFRTIAAAMVSCCFSEAAATSQALQQTLHSRVILKLMIKLINADTNRSMVVP
                     DRVNELAVAMTNAIYRYQKVVRADKQIIPESFSFEFSGHTITITPGRYSRSVNLKVCG
                     GGVPACTTADRTLFRTIAAALVSRNQQGTLNFRSSLPFLAEGNLSA"
     gene            complement(1607..3958)
                     /gene="ygjK"
                     /locus_tag="ACHY5W_004326"
     CDS             complement(1607..3958)
                     /gene="ygjK"
                     /locus_tag="ACHY5W_004326"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4449088.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVLVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQIQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGALKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGSNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEQTQFGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLNKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(3975..5045)
                     /gene="ygjJ"
                     /locus_tag="ACHY5W_004327"
     CDS             complement(3975..5045)
                     /gene="ygjJ"
                     /locus_tag="ACHY5W_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFS4449089.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
BASE COUNT         1104 a         1370 c         1323 g         1278 t
ORIGIN      
        1 ccgcgctcag gtttccttcc gccaggaagg ggagggagga tctgaaatta agtgttcctt
       61 gctggttgcg tgataccagt gctgcggcaa tggtgcgaaa cagagtacgg tcagctgttg
      121 tacaagctgg caccccgccc ccacagacct taaggttcac agagcgacta taccttcccg
      181 gcgtgatggt gatggtatgg ccggaaaact cgaaactgaa cgattcaggg attatctgct
      241 tatcagccct caccaccttc tggtaccgat atattgcgtt cgtcatggca actgccagtt
      301 cgttaacccg gtcaggaaca accatgctac ggttcgtatc cgcgttgatt aattttatca
      361 tcagcttcag aataacccga ctgtgcaggg tctgctgcag ggcctgggaa gttgcagcgg
      421 cctcactgaa acagcatgac accattgccg cggcaatggt gcgaaaccgg gcactgccaa
      481 tacattcacg gatcgtcacc ccgcccctac tgacctcaat ctgcactaat ttgctattac
      541 tctccgcctt cgtaaaggtg atggtgtggc tggaaaaaaa gaaactgaac ctgaacggct
      601 cagtgaacgg ttcagtgctt tgcagctcag acctcaccac cgtctgatac agaaacagcg
      661 cgttcgtcat ggcaactacc agttcgttaa cctcgtccgt atccgcgttg attaaattta
      721 ttatcttatt cagaattccc cagttgtgct gggactgcag caggggctgg gacattatcg
      781 cgtccgtgct gatgctggag ttgcgtggtg ttaccatgaa atgcattcct tccccttgtt
      841 tttcttcctt gagtgaggga aagaaatcat atgttcctgg ctggcgactg tgtgacagca
      901 ttgttgtgac aaaggcgtga aacagggcac agtcagcatc gccacgggca ttccccccgt
      961 ccttgctgac cgcaatgttt acgagattgt ccccgttgta atcccttttc gtgttcgtga
     1021 aggtgatggt gtggccggaa aaacagaaac tgaacggctc aggaatgtac aagatacgtc
     1081 ccggcatgtt tggatactcc ggcagttcgt tcgtcatcgc tgtgtagggc tgcagagcgt
     1141 ccgccatggt agccaccagt gcgttaacct ggtcaggaac acccgtgctg cagtctgtat
     1201 tcctgttgat taaatttatc atctcctcca gacgtgtcct gcagctctcc tggttctcca
     1261 gggccagggg caatgtcata cctgcactgg ctttgatgtg gcatggtgtt accatgtaat
     1321 taattccttt ctctcagggg ggccagatag tgccgtgttt gctcacaggc ccccggagag
     1381 cagctgactc gtttgttcaa ctcaacagct ccggcaccag tcattgaaca gggcgcatag
     1441 ccgtcggaac attacacgtc agacggcaaa tcagtattat tttattaatt ccggtcagtt
     1501 gcattccggt ttttaccgca gtttaaaaga aaatagtgtg tataccccga tgccgcgcga
     1561 ccagggcgga taaggcgttt acgccgcatc cgccagccgt tgcaaattat tgcttacgga
     1621 aaaaatcgtt atacaacata tacaaatgtg cggcgctcca ggagaaattc ggtgcgcctt
     1681 gctgcgcgcc agtcagcggg ttgtaattct cctgaattgg gccatcggcg gttaatcctt
     1741 tggcatgccg gaagaacgta tccgccagct tcagggcatc atcacgataa ccgtaacgct
     1801 ccatcccttt cagaccaaac cagaactgat ccacccatac gcgcccgcgc cagtagatat
     1861 cagcgccaaa agccggattg gttaacgccg ccgttcccag cgggacaaag gtgttgaact
     1921 ctttaggatc gagcatcacc ttcaccaccg cgtcggcatt ggcctgcgtt gccgcaccgt
     1981 taaacagcgg cgaccagcct tccggccctt taccgcgctc aacaatcggt ttgcccgcgc
     2041 agccgttcgc cagcggttta tcttcaatac gcacgtcata gaagtactgc gtagccgggt
     2101 cgaacataca ggtgttgatg tagtccgcga gctgctgtgc caactggcga tagcgcttag
     2161 cctcttccgg tttaccgaga atcgtcgcca tctccgccag ataatggtta tcgctgtaca
     2221 tatagctggc ctgatccacc gactcctgca atagcgagta ccccagcaac gttccgtcct
     2281 gactgcggtt ttcggcgaat ttcaccgtcc agtcgctacg tttgccgccg cttgcaacat
     2341 atttattcag ctgttctttg tcgataaaac caaagacggc ggcgtcatca cggcccgatt
     2401 cccacgatgc agcaacctgt gccggaattt ccagactgtc gtactggcct ttctccacca
     2461 cgcgggcgta gttgttcagc ccaaactgcg tctgttcttt gttgcctttc ttcaccgtaa
     2521 acagcatctc gccgctctca gtgttgtggg ctttgtcgcg ggtcgcgcca tattccggca
     2581 cgccgttgcc gttgtgatcg cggttacgta accaccagtc gtgataggcc accagtttcg
     2641 ggtacatctc tgccagccag gttttatcct gggtgacgtt atacacttcc atcaccgacc
     2701 aggcggcaag gctgggtttg gtattacgtt cgttccagtt gctgccatcg ccaccacgct
     2761 cggggctaag gttccacgct atcaggtcgg ggacaaagcc cacatcctgc ggacgcacgc
     2821 ggtcgccggg ctggatctgc caggagaaga ccgcgcggat attctctttg gcgatgtccg
     2881 gattgaaatg cgccatcgca aacgcctgct tccaggtatc ccacggccag gtctgattgc
     2941 cggagaacca gcgcccggtc accgacggcg tgacggtgtt gtatttcaac gcgccgccag
     3001 gcgagcgcca gttaccgttg agcgtttcga tcgctttcac cgcgacgcgc gtctgttccg
     3061 gcgtcgcatc cggattggtt aaccctttct tcagatactc ttcccagcgt tgctgcgagg
     3121 cggtgagata aaacgccgga cgcgccagaa tatcgcggat ctgtatttgc tctttgctaa
     3181 cttcctgagc ggtcagcagg tgggagtagg tggtatagag cgtcgtcgac ccgttgatat
     3241 gcgccttact ggtaaagcga ttaccgttga tttcagtctg taccggcagg gatttatgca
     3301 cctgatattc cgattcgccg gaggtcagca gatcccaggt ggcgcgcact ttgccaaagg
     3361 tgactttcag gccatcacgg gtggcactga ttttgcgctg gtagtcaggg tattcgccag
     3421 caatggtttt atcggaaagc ggtttccctt ctttcgcttc cagtttttcc agcagttcgc
     3481 catcccacac cagatccagc ggtttattgc tgatgatttt agtttccagt agtgacgtgc
     3541 gcggcgtggc gaagcgcaga gtcatttcga ccagcacatc ttttgctgtc agtttttgca
     3601 ccagcgcgcc gggaatactg tatgcctcca gcgtgaagtc gactttcttg ccgtcctgcc
     3661 agacggtcag gcggtcgaaa ttgctggcca taaagttgat gtactcttcc gtcagcagcg
     3721 caacgcctgg aaaaccgccc atggtgttag ggccgtctgg caacagatga ccatgccagg
     3781 ctccgagatc gaaaaacgga ttaaaacgct ggtgatcgtc gtaatcgtaa tccttcatgt
     3841 actgcggcgc accagtacgg ttaatcacgt ttttataatt gtcggcgtta gcggcatggg
     3901 cggaaaaact tatcagcaga gcgcaggtta ctggcgttaa aatagttttg attttcatga
     3961 aattacagtc cttattacca gtagaaatat tgcatcagga agatttggtc gctgggatcg
     4021 ttagaggcat ttttcatgta gaagcggcta tcaaaagccg ttgcgaaacg tccgccgcca
     4081 tattcgtacc agataccaaa ctgcatgaaa gaggtggttt catcgtcagc gtggtcaggg
     4141 cgatgtttgg cgtaggtgta agcggtggag agataaacgc cctttaacgc ttcatacatg
     4201 gcgctcacca tcatcccgct ttcacttccc ggtacgtcca gcccgtcggc acgtccggta
     4261 tggtgccagg cgcgagcggc aaagttaggg gtaagcaggc ccgtgagata gagttgtcct
     4321 gtgccgtcgg taatttccag accattcatc caggtgatgc cttcggcaag gtcatactgg
     4381 atgtaaccgt tcatcattgc cggataggtg tatttgtcgt cgtagcgatc gtacttgcca
     4441 aaatgtagcc atgctttgct ttcgtcatga tgtccggcag gtgtggcggt gacgctataa
     4501 cgaaagttgc cgcttaagtt ctgtaccttc agcgcggtca taatgtcgcg ggtattggga
     4561 atgacgtagc ccaaatcggg tgtgaagtcg ccccaccatt gcaggtcatc cagcgaggaa
     4621 tcctgacgca gactgagcat gatttcggtg ccatcattgg tacgataacc gccatagaaa
     4681 cggttaatcc caccttcgaa accaccccag ctatggtcag gcacccaggc attaccgtcg
     4741 tggtcggcct gcacggtcca gccttcgccg taaatcaggc cgaaccagga gccgtacttc
     4801 atctccaggc caccttcaat ataggtgccg tcgctgtatt tatgtttatt ctcaccctct
     4861 aaatccatat atccaccaac ccctaattcg ccgtaaaaac gaaatgaagg agaagatgtc
     4921 gaggtaattg cgggtgtttt tgtgtcgtca tgttctgccg gacgtgcttc ttctcctgcc
     4981 gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc agtgattaat
     5041 ttcattgtgt taatccatga gctaaagaaa aagta
//
LOCUS       DAZUGK010000261         5027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZUGK010000261 DAZUGK010000000
VERSION     DAZUGK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.263"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            271..429
                     /locus_tag="ACHY5W_004328"
                     /pseudo
     CDS             271..429
                     /locus_tag="ACHY5W_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            653..772
                     /locus_tag="ACHY5W_004329"
     CDS             653..772
                     /locus_tag="ACHY5W_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4449090.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            837..>5027
                     /gene="ypjA"
                     /locus_tag="ACHY5W_004330"
     CDS             837..>5027
                     /gene="ypjA"
                     /locus_tag="ACHY5W_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFS4449091.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGVQKVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVHKGGTSNDTTIKGGTSRIEGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVNGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYR
                     SNGLGGHIETGMRFTDGNW"
BASE COUNT         1459 a         1106 c         1318 g         1144 t
ORIGIN      
        1 attattcttc acactacatc cattttagtg tcactaccaa agaaaaaggg actacgtttt
       61 catgtaaccc cttgatttat ttggtgggct agcagtgact gaattggtgc tgtaattggt
      121 tgaaatattt catctaatgt ctggttcaac tttccttagc tgcctgaaaa ttttttgagc
      181 ttacccagca ataacagcgt tacggtgcac cacgcctgac tgaagaactg cgtgctcagg
      241 gttaccactt caacgtaaaa ccgtggcagg aggtccggac tgaaaaaact gctcaggaca
      301 tcatcggcag ggaacgcact gctagtctgt aaactgaacc gactggggcg cagtatgtgg
      361 catcttgtcg tgctgttgga ggagctgtgc gaacgtggta ttaactttcg tgctctggcc
      421 caatctatat ttgcccaaca atggggggac gaatgctgta aaagtaaaac aatctgcgat
      481 ctcaaagtta ttgtgtgatt tttatgtgag cagaagatat tcatcagaaa cgattatgta
      541 aatcatttta ttttgccaac ggcctcattg tcgaaagaaa agaatacgcc cgtgtttcct
      601 gaatagattg acttttttat ccaatcacac ttcagcgcac tgcgtttaaa aaatgcctca
      661 ttcttatgcg gaatatcatc atttcatcat gatgtctttg atgagcggtg aacacaatac
      721 acttgcgctg tctcttcagg atgaatcccc tggtctggtg cctgtgggct gatgttgcag
      781 cagagctaag gtcgcttaaa cgctactcag tattcacttt tcagaggatg aaatttatga
      841 acaggaccag tccccattat tgtcgccgct cagtactttc cttattgata tctgccttga
      901 tatatgcccc gcccgggatg gcggccttta ctactaatgt tattggtgtg gtaaacgatg
      961 agactgtaga tggtgtccaa aaagtagatg aacgaggtac aacaaataac actcatatta
     1021 tcaaccatgg ccggcaggag gtctatggtg ggatatctaa tagtagtatt attgaaactg
     1081 gtggcgagca actagtatca atccatgccg atattaacgg gcaagctaat aatacaacca
     1141 ttaatggagg cagacagtca attgagtacg gaggaatttc tactggcacg ataatcgaaa
     1201 gtggcaatca atatgttcat aaaggtggta ccagcaatga cacgacaatt aagggcggta
     1261 cttcacgcat agaggggggg actgcaaatg gaacaatcat tgatggtggc agccaacgag
     1321 taacaactca ggggcatgtc gacagtacaa cgataaataa gtctggctct caggacatca
     1381 cgcaaggaag tctggcaacg aacacaacca taaatggtgg tcgacagtat gttgaacaga
     1441 gcacagtaga aacaacaacc attaaaaatg gcggtgagca aagagtatat gagagccgtg
     1501 cgctggacac gacgattgaa ggcggaactc agtctctgaa tagtaagtca acggcaaaaa
     1561 atacgcagat ctattctggt ggcacgcaaa ttgttgataa caccagcacc tcggatgtta
     1621 ttgaagttta ttccggtggc gtgcttgatg ttagtggtgg tacggcaaca aatgttaccc
     1681 agcacgatgg tgcaatttta aaaacaaaca ctaacggtac gacggtgaac ggtacgaata
     1741 gtgaaggtgc attctccatc cacaatcacg tggcagacaa tgtgttgctg gaaaacggtg
     1801 gtcatttaga cataaacgca tatggttcgg caaacaagac gattatcaaa gataaaggaa
     1861 caatgtcagt tttaaccaat gctaaagctg atgcgacccg aatagataat ggcggggtta
     1921 tggatgttgc cggaaacgcg acaaatacca taattaatgg tggcacacag aatattaata
     1981 attatggcat agccacaggc accaatatca acagcggaac gcaaaatatc aaaagcggcg
     2041 ggaaagctga cacaacaatt atatcctccg ggagccggca ggttgttgag aaagatggta
     2101 cggcaattgg cagcaatatt agcgccggag gctcgctgat tgtctatacc ggcggtattg
     2161 cacatggggt taaccaggag acgggcagtg ctttagttgc caacacgggt gcagggactg
     2221 atatcgaagg atacaacaag ctctctcact tcactattac cggaggggag gctaattatg
     2281 ttgtgctgga aaataccggc gaactgactg tagtggctaa aacctcggcg aaaaatacta
     2341 ccattgatgc tggcggtaag ctgattgtcc agaaggaggc taaaacagat agcaccagac
     2401 ttaataatgg cggcgttctg gaggttcagg acggtggtga ggctaagcat gttgagcaac
     2461 aatccggcgg cgcattaatt gcttccacga cctccggaac acttatcgaa ggaaccaaca
     2521 gttatggtga tgctttctac atcaggaatt cagaagctaa aaatgtagtg ctggaaaatg
     2581 ctggctcatt aacagtcgtc actggttccc gggcagttga tacgattatt aatgccaacg
     2641 gcaaaatgga tgtttatgga aaagatgttg gcactgtact caatagtgct ggcacccaaa
     2701 caatatatgc cagtgccact tctgataaag caaatatcaa aggtggcaag caaacggtat
     2761 atggattagc cactgaggca aatatcgaaa gtggtgaaca aattgttgat ggtgggtcaa
     2821 cagataaaac gcacatcaat ggcggtacgc aaaccgttca gaattatggt aaggcaatca
     2881 ataccgatat cgtctctggc ctacaacaaa ttatggcaaa cgggacagcg gaaggttcca
     2941 ttattaatgg gggttcacag gtagttaatg agggcggtct ggctgaaaac tcggtgctta
     3001 atgacggcgg cacactcgat gtgcgggaga aaggcagcgc aacggggata cagcagagta
     3061 gccagggcgc tttggttgca actaccaggg cgacgcgggt cacaggaaca cgcgcggatg
     3121 gcgtcgcgtt cagcatcgag cagggtgcgg cgaacaatat cctgctggca aatggcggcg
     3181 tgttaaccgt ggagtcagac acctcttctg acaaaacaca ggtcaatacg ggcggacggg
     3241 agatcgtcaa aacaaaagcc actgcgacag gcacgacgct cactggcggt gaacaaattg
     3301 tcgagggtgt ggcgaatgag acaacaatta acgacggcgg aatacaaaca gtttcagcta
     3361 acggagaggc aataaaaaca aagatcaatg aaggcggtac gctgacagtc aacgataatg
     3421 gcaaagcgac agatatcgtc cagaacagcg gtgccgctct ccagacgagc acggctaacg
     3481 gtattgaaat cagcggtact caccagtacg gcactttttc catttccggc aatttagcga
     3541 ccaatatgtt gctggaaaat ggcggtaatt tattggtatt agcaggtacc gaagctcgcg
     3601 actccacggt tggcaagggg ggggcaatgc aaaacctggg tcaggactcc gccacaaagg
     3661 ttaactctgg tgggcaatat acccttgggc ggtcaaaaga tgagtttcag cctctggccc
     3721 gggcagaaga tctccaggtc gctggcggga cggcaatcgt ctacgcaggt acgctggcgg
     3781 atgcatcggt cagtggcgcg acaggaagcc tgtcgttaat gacgccacgg gataatgtta
     3841 cgccagttaa actcgaaggg gcggtccgga ttaccgatag cgcgacattg actctgggaa
     3901 atggcgtcga tacaacgctt gccgacctga cggctgccag ccggggcagt gtctggctta
     3961 acagcaataa ttcctgtgca ggcaccagca actgcgagta tagagtaaac agtttgctac
     4021 ttaacgacgg taatgtttat ttatcagcac aaacagcagc gcctgccaca actaacggta
     4081 tctacaatac gctgacaact aatgaacttt ccggtagcgg taatttctac ctgcatacca
     4141 acgttgcagg ctcccggggc gatcaactgg tcgtcaacaa caacgccact ggtaatttta
     4201 aaatctttgt tcaggatacc ggcgtcagcc cacagtctga cgacgcgatg acgctggtga
     4261 aaacaggggg aggggatgct tcgtttacgc tgggcaatac cggcggtttc gttgatcttg
     4321 ggacctatga gtatgtcctg aaaagcgacg gcaacagcaa ctggaacctg accaatgatg
     4381 tcaaacccaa cccggatccc aacccaaatc ccaacccaaa tccgaagccg gatccaaaac
     4441 cagacccaaa accggatccg aaaccagacc cgactcccga tccaacgccg acacccgttc
     4501 cggagaaacg catcacgcct tctacggcag ccgtactcaa tatggctgca acattaccgt
     4561 tggtatttga tgctgagcta aacagtattc gcgagcggtt gaacataatg aaagcgagtc
     4621 cacacaacaa taatgtctgg ggggcgacgt ataacacccg taataatgtc accaccgatg
     4681 cgggtgccgg gtttgagcag acgctgaccg gaatgacagt ggggatcgac agccgtaatg
     4741 atattcctga aggaattgcg acgctgggcg cttttatggg ttattcccat tcacatatcg
     4801 gttttgatcg tggaggacat ggcagtgtgg gcagttattc tctgggcggc tatgccagtt
     4861 gggaacatga aagtggtttc tatctggacg gtgtcgtgaa gctgaaccgt tttgaaagta
     4921 acgtagccgg taaaatgagc agcggtggag ccgccaacgg cagttatcgc agcaacgggc
     4981 tgggcggtca cattgaaacc gggatgcgat ttaccgatgg taactgg
//
LOCUS       DAZUGK010000262         4968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZUGK010000262 DAZUGK010000000
VERSION     DAZUGK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.264"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            237..1181
                     /gene="leuO"
                     /locus_tag="ACHY5W_004331"
     CDS             237..1181
                     /gene="leuO"
                     /locus_tag="ACHY5W_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFS4449092.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFADSLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1336..1380)
                     /locus_tag="ACHY5W_004332"
     CDS             complement(1336..1380)
                     /locus_tag="ACHY5W_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449093.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1499..3223
                     /gene="ilvI"
                     /locus_tag="ACHY5W_004333"
     CDS             1499..3223
                     /gene="ilvI"
                     /locus_tag="ACHY5W_004333"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFS4449094.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYSFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3226..3717
                     /gene="ilvN"
                     /locus_tag="ACHY5W_004334"
     CDS             3226..3717
                     /gene="ilvN"
                     /locus_tag="ACHY5W_004334"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS4449095.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3729..3815
                     /locus_tag="ACHY5W_004335"
     CDS             3729..3815
                     /locus_tag="ACHY5W_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFS4449096.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3897..4901
                     /gene="cra"
                     /locus_tag="ACHY5W_004336"
     CDS             3897..4901
                     /gene="cra"
                     /locus_tag="ACHY5W_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFS4449097.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLNRS
                     "
BASE COUNT         1211 a         1128 c         1312 g         1317 t
ORIGIN      
        1 gtagttatga ttagattgtt ttcgcaacaa aaacattatg gattattatg ctgtggtaag
       61 tgacgctttc cacggcaatg gattctgttt ttataagaac ccgtatcttt atgttttcct
      121 aattttactc attttccttt ttcttatttt atatgcatga taaatcatat tctcgaggat
      181 tatttctctg cattccaata agggaaaggg agttaagtgt gacagtggag ttaagtatgc
      241 cagaggtaca aacagatcat ccagagacgg cggagttaag caagccacag ctacgcatgg
      301 tcgatctcaa cttattaacc gttttcgatg ccgtgatgca ggagcaaaac attacccgtg
      361 ccgctcatgt tctgggaatg tcgcaacctg cggtcagtaa cgctgttgca cgcctgaagg
      421 tgatgtttaa tgacgagctt tttgttcgtt atggccgtgg tattcaaccg actgctcgcg
      481 catttcaact ttttggttca gttcgccagg cattgcaact agtacaaaat gaattgcctg
      541 gttcaggttt tgaacccgcg agcagtgaac gtgtatttca tctttgtgtt tgcagcccgt
      601 tagacagcat tctgacctcg cagatttata atcacattga gcagattgcg ccaaatatac
      661 atgttatgtt caagtcttca ttaaatcaga acactgaaca tcagctgcgt tatcaggaaa
      721 cggagtttgt gattagctat gaagacttcc atcgtcctga atttaccagc gtgccattat
      781 ttaaagatga aatggtgctg gtagccagca aaaatcatcc aacaattaaa ggcccgttac
      841 tgaaacatga tgtttataac gaacaacatg cggcggtttc gctcgatcgt ttcgcgtcat
      901 ttagtcaacc ttggtatgac acggtagata agcaagccag tatcgcgtat cagggcatgg
      961 caatgatgag cgtacttagc gtggtgtcgc aaacgcattt ggtcgctatt gcgccgcgtt
     1021 ggctggctga agagttcgct gattccttag aattacaggt attaccgctg ccgttaaaac
     1081 aaaacagcag aacctgttat ctctcctggc atgaagctgc cgggcgcgat aaaggccatc
     1141 agtggatgga agagcaatta gtctcaattt gcaaacgcta actgaatgca gaataggtca
     1201 gacatgaatg tctggtttat tctgcatttt ttattgaatg tggaatttta ttctgaatgt
     1261 gtaggctctc tattttagga ttaattaaaa aaatagagaa attgctgtaa gttgtgggat
     1321 tcagccgatt tattatcaat ttaatccttc gtaatggagg attttatcgt ttcttttcac
     1381 ctttcctcct gtttattctt attatccggc gtttatgtct ctggctgcca attgcttaag
     1441 caagatcgga cggttaatgt gttttacaca ttttttccgt caaacagtga ggcaggccat
     1501 ggagatgttg tctggagccg agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca
     1561 agtattcggt tatcccggag gcgcagtcct tgatatttac gatgcattgc ataccgtggg
     1621 tggtattgat catgtattag ttcgtcatga gcaggcggcg gtgcatatgg ccgatggcct
     1681 ggcgcgtgcg accggggaag tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa
     1741 tgcgattacc ggcatcgcca ccgcttatat ggattctatt ccattagttg tcctttccgg
     1801 gcaggtagcg acctcgttga taggttacga tgcctttcag gagtgcgaca tggtggggat
     1861 ttcgcgaccg gtggttaaac acagttttct ggttaagcaa acggaagaca ttccgcaggt
     1921 gctgaaaaag gctttctggc tggcggcaag tggtcgccca ggaccagtgg tcgttgattt
     1981 accgaaagat attcttaatc cggcgaacaa attaccctat gtctggccgg agtcggtcag
     2041 tatgcgttct tacaatccca ctactaccgg acataaaggg caaattaagc gtgctctgca
     2101 aacgctggta gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa tcacggcggg
     2161 ctgccatcag cagttgaaag aaacggtgga ggcgttgaat ctgcccgttg tttcctcatt
     2221 gatggggctg ggggcgtttc cggcaacgca tcgtcaggca ctgggcatgc tgggaatgca
     2281 cggtacctac gaagccaata tgacgatgca taacgcggat gtgattttcg ccgtcggggt
     2341 acgatttgat gaccgaacga cgaacaatct ggcaaagtac tgcccaaatg ccactgttct
     2401 gcatatcgat attgatccta cttccatttc taaaaccgtg actgcggata tcccgattgt
     2461 gggggatgct cgccaggtcc tcgaacaaat gcttgaactc ttgtcgcaag aatccgccca
     2521 tcaaccactg gatgagatcc gcgactggtg gcagcaaatt gaacagtggc gcgctcgtca
     2581 gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg caggcggtga tcgagactct
     2641 ttggcggttg acgaagggag acgcttacgt gacgtccgat gtcgggcagc accagatgtt
     2701 tgctgcactt tattattcat tcgacaaacc gcgtcgctgg atcaattccg gtggcctcgg
     2761 cacgatgggt tttggtttac ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac
     2821 cgtggtttgc gtcactggcg acggcagtat tcagatgaac attcaggaac tgtctaccgc
     2881 gttgcaatac gagttgcccg tactggtggt gaatctcaat aaccgctatc tggggatggt
     2941 gaagcagtgg caggacatga tctattccgg ccgtcattca caatcttata tgcaatcact
     3001 acccgatttc gtccgtctgg cggaagccta tggtcacgtc gggatccaga tttctcatcc
     3061 gcatgagctg gaaagcaaac ttagcgaggc gctggaacag gtgcgcaata atcgcctggt
     3121 gtttgttgat gttaccgtcg atggcagcga gcacgtctac ccgatgcaga ttcgcggggg
     3181 cggaatggat gaaatgtggt taagcaaaac ggagagaact tgattatgcg ccggatatta
     3241 tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct tttttcccag
     3301 cgtggctaca acattgaaag cctgaccgtt gcgccaaccg acgatccgac attatcgcgt
     3361 atgaccatcc agaccgtggg cgatgaaaaa gtacttgagc agatcgaaaa gcaattacac
     3421 aagctggtcg atgtcttgcg cgtgagtgag ttggggcagg gcgcgcatgt tgagcgggaa
     3481 atcatgctgg tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg
     3541 gaaatattcc gtgggcaaat tatcgatgtc acaccctcgc tttataccgt tcaattagca
     3601 ggcaccagcg ataagcttga tgcattttta gcatcgattc gcgatgtggc gaaaattgtg
     3661 gaagttgctc gctctggtgt ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa
     3721 tgatctcaat gtgcaatttg cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag
     3781 tgggaacctg gaataaaagc agttgccgca gttaattttc tgcgcttaga tgttaatgaa
     3841 tttaacccat accagtacaa tggctatggt ttttacattt tacgcaaggg gcaattgtga
     3901 aactggatga aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc tatgttatta
     3961 acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg
     4021 tgcgtgagca caattaccac ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac
     4081 gttctattgg tcttgtgatc cccgatctgg agaacaccag ctatacccgc atcgctaact
     4141 atcttgaacg ccaggcgcgg caacggggtt atcaactgct gattgcctgc tcagaagatc
     4201 agccagacaa cgaaatgcgc tgcattgagc accttttaca gcgtcaggtt gatgccatta
     4261 ttgtttcgac gtcattgcca cctgagcatc ctttttatca acgctgggct aacgacccgt
     4321 tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca ctttaccagc gtggttggtg
     4381 ccgatcagga tgatgccgaa atgctggcgg aagagttacg taagtttccc gccgagacgg
     4441 tgctttatct tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa caaggtttcc
     4501 gtactgcctg gaaagatgat ccgcgcgaag tgcatttcct gtatgccaac agctatgagc
     4561 gggaggcggc tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc
     4621 tgttcacaac gtcgtttgcg ttgttgcaag gagtaatgga tgtcacgctg cgtcgcgacg
     4681 gcaaactgcc ttctgacctg gcaattgcca cctttggcga taatgaactg ctcgacttct
     4741 tacagtgccc ggtgctggca gtggctcaac gtcaccgcga tgtcgcagag cgtgtgctgg
     4801 agattgtcct ggcaagcctg gacgaaccgc gtaagccaaa acctggttta acgcgcatta
     4861 aacgtaatct ctatcgccgc ggtgtgctca accgtagcta agccgcgaac aaaaatacgc
     4921 gccaggtgaa tttccctctg gcgcgtagag tacgggactg gacatcaa
//
LOCUS       DAZUGK010000263         4955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZUGK010000263 DAZUGK010000000
VERSION     DAZUGK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.265"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1418)
                     /locus_tag="ACHY5W_004337"
     CDS             complement(<1..1418)
                     /locus_tag="ACHY5W_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS4449098.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWG
                     VDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRAD
                     LADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTD
                     ENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLAT
                     AILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSAS
                     GGRAEVVSDAEGTGGKAVKLTK"
     gene            1659..1793
                     /locus_tag="ACHY5W_004338"
                     /pseudo
     CDS             1659..1793
                     /locus_tag="ACHY5W_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1898..1968)
                     /locus_tag="ACHY5W_004339"
     tRNA            complement(1898..1968)
                     /locus_tag="ACHY5W_004339"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Met,seq:cat)
     gene            complement(2131..2688)
                     /locus_tag="ACHY5W_004340"
     CDS             complement(2131..2688)
                     /locus_tag="ACHY5W_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS4449099.1"
                     /translation="MNNVSVENLPSLTHQHLPVITTELLAELYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDELRELKHRNSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFSQKDPSTPVSCQKSYDTRVLCYQQGGVTVSTIQLRDDDIVISLES
                     WLELARANGWFVVRRDKLVERLMQL"
     gene            complement(3168..3722)
                     /locus_tag="ACHY5W_004341"
     CDS             complement(3168..3722)
                     /locus_tag="ACHY5W_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4449100.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDSHPEWCYATCRNRIDAWL
                     KMAEFMLYLPMRDAFSSGDLKTVC"
     gene            complement(3719..4009)
                     /locus_tag="ACHY5W_004342"
     CDS             complement(3719..4009)
                     /locus_tag="ACHY5W_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS4449101.1"
                     /translation="MVDLRKAAKGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(4009..4608)
                     /locus_tag="ACHY5W_004343"
     CDS             complement(4009..4608)
                     /locus_tag="ACHY5W_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS4449102.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(4680..4892)
                     /locus_tag="ACHY5W_004344"
     CDS             complement(4680..4892)
                     /locus_tag="ACHY5W_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449103.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1196 a         1361 c         1129 g         1269 t
ORIGIN      
        1 tccttggtca gcttcactgc cttacctccg gttccctccg catcgctgac cacttctgcc
       61 ctgccgccac tggctgacca tccctgtact ttcaggcttc cctctgactc gctggcaagg
      121 taagagagca gtgttgtgac gcctgtggct tctgcaccgg aaggcgatga cgggcgcacc
      181 tctgatactg tcgatgatgc ccccgcgttt agcgccactc ttcccgcatg gcgcaaaatc
      241 gccgttgcca gacggtcgga aataatcccg cggcgagccc atgaactgaa atggctcgcc
      301 ctgtcctgtg acgtccaggt ggctgagctg tcacgccatt tcgaaccgta atatccgata
      361 cccggaatgt ccgggtcttc ttccggtttg ttcgtcggca cattcacccc gttctcatcc
      421 gtcatgaacg gtacgaaatg gatattcttt tccgttttgt ttttatagct gccatacacc
      481 gtctggtacg tggattcgtt cttctgcttc cagaaatacg tcgtgtcccc gcatatccag
      541 ggaacaccgc cagcagagcc accgacgcac tggcctgcca tatccgccag gtctgcacgg
      601 aatttatcaa ccagcgcacc aaactgtgct gcgtgatttg ccggcgtacc gccaaaatca
      661 aattccccct gcatccacac cacggcaaac agcacatttt tcggattctt cttcagtgct
      721 gcttttgttc gaccgataag gtccttatac agcggcttgt ccacacccca gcgggttgaa
      781 ttctccgagg caccgccagc gtcactgtat gtgccatcgg ctccggtggt gaacgctgaa
      841 ccaccacgac agcacggaac cagcagaatg cccgcattcg ccggtataaa cggcagcagt
      901 tttttggcaa tatgcagccc ctgccccacg gtaccgtact gcccctttga caggtccgct
      961 ttcggatggt taaggcggct catgtcctgc acatcatgca gacaatggtc cgccggaatg
     1021 atgtcgttat acttacaggc ggcaccgccc ggtgtcaccg tactgcgacg cgccagctgc
     1081 ttaatgcgcg ggtccggacg gtcatatgtc tccggcagcg gaaggccttc accatatgcc
     1141 atgctgtttg actgccccgc cagaaccaca acaaagtaat actccgggtc tctggtggcg
     1201 ctgattactg tgccctctcc atccgacggc ttcaccacaa caggtgtgct cacatcacct
     1261 tctgcgacaa tcgcctgaat aagtgctgcg ccatcatccg tatacgaaga aaacggcccg
     1321 ccgtatggct gccatccttc acgaattttt tgagcaagcg catccgcaag gtctgacggc
     1381 gacgccgccc tgacaacatc ataatgttta aatgtcatta ttcctcccgg ccgggatagt
     1441 gtattaaatc agatatggag tgggctgtag tccggaagcc tgaatgacac acggggacta
     1501 cagcccaaga aatgaaaaaa ggccacgcag ttgcgcagcc tgataaaccc tggttaaaat
     1561 ccacacgata acaacacaac aatatcagta tctcatgcta ttgcccgaac ccattcgggc
     1621 attttttacc cataaaaaat gcccctccgg agaggggcat gtttgcatgc acattctttt
     1681 tcttgcatgg tgccgggtgc ctcccggtga attcagtacc agcacctgaa tccgcgatta
     1741 tcccatatac ctactcgctg attgcccctc cgcacagggg gattcaccat gccagtttct
     1801 tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa
     1861 aaaaaggccc gcaaaagcga gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa
     1921 ccagcgacct ggcgattatg cgtcgctcgc tctcaccact gagctaaagg gccgggagca
     1981 gaataataac ggtccgtaat taattccgca ataaaaaacc cgctcaatgg cgggttctgg
     2041 taaagttcat gcgcttggtt cgcctcgcga tacagctttg cgaagcgtac cggaattgga
     2101 gcagtttatg gctaaaattg caagaacttt ttaaagctgc atcagccttt ccaccagttt
     2161 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
     2221 aataacaata tcatcatccc gcaactgaat tgtggaaaca gtgacaccgc cttgctgata
     2281 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccttttg
     2341 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
     2401 tttggcgtga cgggctgctc cgcgttctgt ccagaggatg agggagcgaa cgttacgggc
     2461 aattttcaca gaaaccctta aagagtttct gtgcttcagt tcccgtaatt catcgccttc
     2521 gatcaggaaa aagtgctttc cttcaataaa ccgctctttg tttctattga aattatttgt
     2581 caaacgctgg cgttctgttc cgtacagctc agccagaagt tctgttgtta taacaggaag
     2641 gtgttgatgt gtaagtgatg ggaggttttc aacagatacg ttgttcatga atatgtccta
     2701 ctgaatattt tgatttaccc cttttgagag ggtgaccggg cgctcaaaac cgtcagtagt
     2761 cggcgggcgt attcccattg caggtattgt attagccgca cgcccggtca taaaccaaga
     2821 attctggaca taaaaaaacc gcatgtctgt cgggcgcggt aaccgctact gaaaaggtgt
     2881 tttgagcacc gccaaggaat gtaatctgct aacatgaaac tgtcaataca ttcatgttcc
     2941 aatcatcgtg gtggcgaact gtgcagggtt agcactaccg ggtacggaaa ccggcgagcc
     3001 tttcggctcc cccacacagc ccgccataaa tcgcgaatgt gactgtgcaa acgatatgaa
     3061 aaaagacgcg ggcgcgtctc atatcgctcc gtaaacatcc ggggtgctaa tcccgacgcc
     3121 agattttgct ggcgcgtgag gaatatagcc ccggataaca gattgagtca acagacggtt
     3181 tttagatccc cggaagagaa tgcatcacgc atcggcagat agagcataaa ctctgccatt
     3241 ttcaaccatg catctatgcg attacggcac gtggcgtaac accactcagg gtgtgaatca
     3301 ttcagcaatt cagccatttt tcgcttagtc atccccctcc cttcatatcg ttgccggagg
     3361 atacaaatca atcctgggtg ttctgccagc acttcactaa tcacacgatc aatgcataac
     3421 gcctctgcat cagtacaatg cgccaaccag cttttttgct taccgttgat catatcccgc
     3481 aaaaaagcct caagttcagg tttgtccaga cctgcttttt tcatcctccg gagcgcctcg
     3541 ttaattgccg tttttgtcag ctttttagag gtcagcaact ggttgaacat atttcccgtc
     3601 ttaccgccgc caatatacga ccagcgcccc cacatgcgca gttttccctg aatccagaca
     3661 ctttccagcg tggtgagacg aaggtgttcc ccgcttttgc ctgtatttgt tgggtaaatc
     3721 ataaataacc ttcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca
     3781 ggcgcaattc ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc
     3841 acgagctaca ggcaattgcc gcctgcatat cgtgtggttt tgtcgctgtt ccacacgtcc
     3901 ccgccagcct gtaatgcgcc agcacggacg tttcgggatt gtgattgcag tagccaggaa
     3961 ttctgactgt acacatctga ccttttgccg ctttacgtaa atccaccatt acgcaaactc
     4021 cagtagctgc gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacaggat
     4081 ccagttccac agtacattca gcacagattt ataaacctgc tgaaactcgg tttcgtccat
     4141 attcgcaaac gcgatggatt tcgccctgcg cccacggcta ccgtccggat aaaaatgctc
     4201 ggtgtaaaat ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa
     4261 tgccgtatcc cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact
     4321 cagggctgga gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac
     4381 cagttttcgt tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag
     4441 ttgcaggagt ttgaaaaaac gcttgtggaa cgcgtagtta cgcactcgct taaagtccgc
     4501 gtgtatccac tcacctattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg
     4561 gagaagtaat ccggaagagg tttgtttgac cagttgtata tgcgccatcg tagttctccg
     4621 ctggcgcagt agaatgggtg ttcagcccgt tatgtagtat aacagaatta atgccaatac
     4681 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
     4741 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttggggtt
     4801 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
     4861 ttgctactaa atagtttttt tattttttcc acgatgcctc caggtttata agtacaaacg
     4921 gttatattca catagagaca aaaatattaa tctga
//
LOCUS       DAZUGK010000264         4851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZUGK010000264 DAZUGK010000000
VERSION     DAZUGK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.266"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..692
                     /gene="fimC"
                     /locus_tag="ACHY5W_004345"
     CDS             3..692
                     /gene="fimC"
                     /locus_tag="ACHY5W_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS4449104.1"
                     /translation="MTKIKLLMLIIFYLIISARAHAAGGIALGATRIIYPADAKQTAV
                     WIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADR
                     ESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRS
                     GNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYG
                     SVTPVREVNLN"
     gene            723..3332
                     /locus_tag="ACHY5W_004346"
     CDS             723..3332
                     /locus_tag="ACHY5W_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS4449105.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPASVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNEHIVGLNVSVPFNVLTKRRYTRENALDRAYASLNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3345..4352
                     /gene="fimH"
                     /locus_tag="ACHY5W_004347"
     CDS             3345..4352
                     /gene="fimH"
                     /locus_tag="ACHY5W_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS4449106.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            4363..>4851
                     /gene="sfmF"
                     /locus_tag="ACHY5W_004348"
     CDS             4363..>4851
                     /gene="sfmF"
                     /locus_tag="ACHY5W_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFS4449107.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLTAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KAD"
BASE COUNT         1246 a         1064 c         1206 g         1335 t
ORIGIN      
        1 tgatgactaa aataaagtta ttgatgctca ttatatttta tttaatcatt tcggccagag
       61 cccatgctgc cggagggatc gcattaggtg ccacgcgtat tatttatccc gctgatgcta
      121 aacagactgc ggtatggatt agaaatagcc ataccaatga gcgctttctg gtcaattcgt
      181 ggattgaaaa cagcagcggt gtaaaagaaa agtcattcat cattacaccg ccactgtttg
      241 ttagtgaacc caaaagcgaa aatactttgc gtattattta caccggtcca ccgctggcag
      301 cagatcgtga gtctctgttc tggatgaatg ttaagacgat cccttcggta gataaaaatg
      361 cattgaacgg caggaatgtt ttgcaactgg cgattttatc gcgcatgaag ttatttctcc
      421 gtccaattca actacaagaa ttacccgcag aagcgccgga cacactcaag ttttcgcgat
      481 ccggtaacta tatcaatgtt cataatccat caccttttta tgtcaccctg gttaacttac
      541 aagtgggcag ccaaaagttg gggaatgcta tggctgcacc cagagttaat tcacaaattc
      601 ccttaccctc aggagtgcag ggaaaactga aatttcagac cgttaatgat tatggttcag
      661 taaccccggt cagagaagtg aacttaaact aaccgaatca tctgacaata tcagagctaa
      721 ttatgaaaat acccactact acggatattc cgcagaggta tacctggtgt ctggccggaa
      781 tttgttattc atctcttgcc attttaccct cctttttaag ctatgcggaa agttatttca
      841 acccggcatt tttattagag aatggcacat ccgttgctga tttatcgcgc tttgagagag
      901 gtaatcatca acctgcgagt gtgtatcggg tggatctctg gcgtaatgat gagttcattg
      961 gttcacagga tatcgtattt gaatcgacaa cagaaaatac aggtgataaa tcaggtgggt
     1021 taatgccctg ttttaaccag gtactccttg aacgaattgg ccttaatagc agtgcatttc
     1081 ccgagttagc ccagcagcaa aataataaat gtattaattt actgaaagct gtacctgatg
     1141 ccacaattaa ctttgatttt gcagcgatgc gcctgaacat cactattcca cagatagcgt
     1201 tgctgagtag cgctcacggt tacattccgc ctgaagagtg ggatgaaggt attcctgctt
     1261 tactcctgaa ttataatttc accggtaaca gaggtaatgg taacgatagc tattttttta
     1321 gtgagctcag cgggattaat attggcccgt ggcgtttacg caacaatggt tcctggaact
     1381 attttcgcgg aaatggatat cattcagaac agtggaataa tattggcacc tgggtacagc
     1441 gcgccattat cccgctgaaa agtgaactgg taatgggaga cggcaataca ggaagtgata
     1501 ttttcgatgg cgttggattt cgtggtgtac ggctttactc ttccgataat atgtatcctg
     1561 atagccagca ggggtttgcc ccaacggtac gtgggattgc ccgtacggcg gcccagctaa
     1621 cgattcggca aaatggtttt attatctatc aaagctatgt ttcccccggc gcttttgaaa
     1681 ttacagattt gcacccgaca tcttcaaatg gcgatctgga cgtcaccatc gacgaacgcg
     1741 atggtaatca gcagaattac actattccgt attcaacagt gccaatttta caacgcgaag
     1801 ggcgtttcaa atttgacctg acggcgggcg attttcgtag cggtaatagt cagcaatcat
     1861 cgcctttctt ttttcagggc acggcactcg gcggtttacc acaggaattt actgcctacg
     1921 gcgggacgca attatctgcc aattacaccg cctttttatt agggctgggg cgcaacctcg
     1981 ggaactgggg cgcagtgtcg ctggatgtga cccatgcgcg cagtcagtta gccgacgaca
     2041 gtcgtcatga gggggattcc attcgcttcc tctatgcgaa atcgatgaac accttcggca
     2101 ccaattttca gttaatgggt taccgctatt cgacacaagg tttttatacc cttgatgatg
     2161 ttgcgtatcg tcgaatggag gggtacgaat atgattacga ttacgacggt gagcatcgcg
     2221 atgaaccgat aatcgtgaat taccacaatt tacgctttag ccgtaaagac cgtttgcagt
     2281 taaatatttc acaatcactt aatgactttg gctcgcttta tatctctggt acccatcaaa
     2341 aatactggaa tacttcggat tcagatacgt ggtatcaggt ggggtatacc agcagctggg
     2401 ttggcatcag ctattcgctc tcattttcgt ggaatgaatc tgtagggatc cccgataacg
     2461 aacatattgt cggacttaat gtttcagtgc ctttcaatgt tttgaccaaa cgtcgctaca
     2521 cccgggaaaa tgcgctcgac cgcgcttatg cctccttgaa cgctaaccgt aacagcaacg
     2581 ggcaaaatag ctggctggca ggcgttggtg ggaccttact ggaaggccac aacctgagtt
     2641 atcacgtaag ccagggcgat acttcgaata atgggtacac gggcagcgcc acggcaaact
     2701 ggcaggccgc ttacggtacg ctgggggtcg ggtataacta cgaccgcgat caacatgacg
     2761 ttaactggca gctgtctggc ggtgtggtcg ggcatgaaaa tggcataacg ctgagccagc
     2821 ctttagggga taccaatgtt ttgattaaag cgcctggcgc aggcggtgta cgcattgaaa
     2881 atcaaactgg cattttaacc gactggcgcg gctatgcggt gatgccgtat gccacggttt
     2941 atcggtataa ccgtatcgcg cttgatacca atacgatggg gaattccatc gatgttgaaa
     3001 aaaatattag cagcgttgtg ccgacgcaag gcgcgttggt tcgtgccaat tttgataccc
     3061 gcataggcgt gcgggcgctc attaccgtta cccagggcgg aaaaccggtg ccgtttggat
     3121 cactggtacg ggaaaacagt accggaataa ccagtatggt gggtgatgac gggcaagttt
     3181 atttaagtgg tgcgccattg tctggtgaat tactggttca gtggggagac ggcgcgaact
     3241 cacgctgcat tgcgcactat gtattgccga agcaaagctt acagcaagcc gtcactgtta
     3301 tttcggcagt ttgcacacat cctggctcat aaaggaaatt atcaatgaag ataatctgca
     3361 gattattatt ggcgatggca tgtttgtgtc tggcaaacat atcctgggct actgtttgtg
     3421 caaatagtac tggcgtagca gaagatgaac actatgatct ctcaaatgtc tttaatagca
     3481 ccaataacca gccagggcag attgttgttt taccggaaaa atccggctgg gtaggtgtct
     3541 cagcaatttg tccacccggc acgctggtga attatacata ccgtagttat gtcactaact
     3601 ttattgttca ggaaactatc gataattata aatatatgca attaaatgat tatctattag
     3661 gtgcgatgag tctggttgat agtgtgatgg atattcaatt tcccccgcaa aattatattc
     3721 gtatgggaac agatcctaac gtttcgcaaa accttccgtt cggggtgatg gattctcgtt
     3781 tgatatttcg tttaaaggtt attcggccct ttattaacat ggtggagatc cccagacagg
     3841 tgatgtttac cgtgtatgtg acatcaacgc cttacgatcc gttggttaca cctgtttata
     3901 ccattagttt tggtggccgg gttgaagtac cgcaaaactg cgaattaaat gccgggcaga
     3961 ttgttgaatt tgattttggt gatatcggcg catcgttatt tagtgcggca gggccgggta
     4021 atcgacctgc tggtgtcatg ccgcaaacca agagcattgc ggtcaaatgt acgaatgttg
     4081 ctgcgcaggc ttatttaaca atgcgtctgg aagccagtgc cgtttctggt caggcgatgg
     4141 tgtcggacaa tcaggattta ggttttattg tcgccgatca gaacgatacg ccgatcacgc
     4201 ctaacgatct caatagcgtt attcctttcc gtctggatgc agctgcggca gccaatgtca
     4261 cacttcgcgc ctggcctatc agtattaccg gtcaaaaacc gaccgagggg ccgtttagcg
     4321 cgctggggta tttacgcgtc gattatcaat gaggtacgga gaatgagaag agtattcttt
     4381 agctgtttct gcgggttact gtggagttcc agtggatggg cagttgaccc tttaggaacg
     4441 attaatatca atttgcacgg taacgttgtt gatttctcct gtaccgtaaa cacagcggat
     4501 attgataaga cggtagattt aggcagatgg cctacgacac aactactgac cgctggtgat
     4561 accacggcac tcgtcccttt tagcctgcgg ctggagggat gtcctccggg ttcagttgcg
     4621 attttattta cgggaacgcc ggcatctgat gccaacctgc tggctctgga tgatcccgca
     4681 atggcacaaa ccgttgccat cgaattacgt aatagcgatc gctcccggct cgcactgggg
     4741 gaggcgagcc cgactgagga agtagatgca aatggcaatg tcacactaaa cttttttgcc
     4801 aattatcgag cgttagccag cggtgttcgg ccaggtgtgg cgaaagcgga t
//
LOCUS       DAZUGK010000265         4831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZUGK010000265 DAZUGK010000000
VERSION     DAZUGK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.267"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..843
                     /gene="ydiV"
                     /locus_tag="ACHY5W_004349"
     CDS             130..843
                     /gene="ydiV"
                     /locus_tag="ACHY5W_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFS4449108.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITHFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            906..2342
                     /gene="selO"
                     /locus_tag="ACHY5W_004350"
     CDS             906..2342
                     /gene="selO"
                     /locus_tag="ACHY5W_004350"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFS4449109.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQQLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFIDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQAEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2346..2537)
                     /gene="hemP"
                     /locus_tag="ACHY5W_004351"
     CDS             complement(2346..2537)
                     /gene="hemP"
                     /locus_tag="ACHY5W_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFS4449110.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2669..3715)
                     /gene="aroH"
                     /locus_tag="ACHY5W_004352"
     CDS             complement(2669..3715)
                     /gene="aroH"
                     /locus_tag="ACHY5W_004352"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFS4449111.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIIGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3872..4705)
                     /gene="ppsR"
                     /locus_tag="ACHY5W_004353"
     CDS             complement(3872..4705)
                     /gene="ppsR"
                     /locus_tag="ACHY5W_004353"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFS4449112.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1200 a         1133 c         1235 g         1263 t
ORIGIN      
        1 aaaaaggacc cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat
       61 tgttatgtct tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg
      121 acggttacca tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg
      181 ataagagata accagcaagt ccttgtcggc gttgagttaa ttacccattt ctccagtgaa
      241 gatggcacgg tgcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat
      301 tggcaattat tttcagagca gttggaatta ctaaaatcat gccaacattt ttttattcaa
      361 cataaactct ttgcctggct taatttaacg ccacaagtgg ctacattatt attagagagg
      421 gataattacg caggcgaact attaaaatat ccgtttattg aactgttgat taatgagaat
      481 tacccgcatc tcaatgaagg aaaagataat cgcggtcttt tatccttatc gcaagtgtat
      541 ccccttgtgt taggtaattt gggagcaggc aatagcacaa tgaaagcggt ttttgatggt
      601 cttttcaccc gcgtcatgct ggataaaagt tttattcagc aacaaatcac gcatcgctcc
      661 ttcgaaccgt ttattcgtgc gatccaggcg caaatttccc cttgttgcaa ttgcattatc
      721 gcggggggca ttgatacgcc agaaatattg gcacagatta cccacttcga tttccatgct
      781 ctccagggat gcctgtggcc tgccgttccc ataaatcaga taacgacgtt ggttcagcga
      841 taaccctcct gtttgctggt gtttaagacg agagtaaccg tctacactat caaacaggag
      901 gatctatgac cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag
      961 cactttcccc tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta
     1021 acacgctgag tattccatcg tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaaa
     1081 ccctactgcc tggtatgtca ccactggcgc aggtttacag tggtcatcag ttcggcgtct
     1141 gggcgggcca actgggtgat gggcgcggca ttttactcgg cgaacaacag ctggccgatg
     1201 gcactacaat ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg
     1261 atggacgggc ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt
     1321 atctgggcat tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc
     1381 gggaaacggt ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg
     1441 gtcatttcga acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact
     1501 ttgccattcg tcattactgg tcacatcttg aagatgatga ggacaaatac cgtctctggt
     1561 ttaacgatgt tgtcgcacgt accgcatcgt taattgccca atggcagacg gtcggctttg
     1621 ctcatggggt gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc
     1681 cgtttggttt tattgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag
     1741 ggcgttatag ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc
     1801 agacattgtc accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc
     1861 aggttttgtt gacgcattat ggacaacgga tgcggcagaa actgggcttc atcacggagc
     1921 aaaaagagga taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg
     1981 attatacccg cacatttcgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc
     2041 tacgtgatga gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgagggc
     2101 gtttgcaaca agccgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc
     2161 ccgctctggt attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg
     2221 atatgacgga attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg
     2281 atgactatgt cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt
     2341 aaaggctact tggtcaacag cagcttgcca gcctgagttt tacggagcag atattcttgc
     2401 ccgtcatgat cgataatcag tttgccatcc ggacctaaca gtgtctggct ggaaattcgc
     2461 cgaataggct gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg
     2521 ctatccgtat aacgcataaa aattaataat aattgatata acaatgattc ttattatcaa
     2581 tattatagct gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat
     2641 gcccatccca tcaggaatgg gcacgcattc agaagcgggt atctaccgca gaggcgagtt
     2701 tttcgaccag gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt
     2761 aagtgagcgg ctgactgccg atgatttttt gcgttccttc gcgcaggaaa ctttccgcca
     2821 taattccagc aatcgccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt
     2881 ctaactggcg acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt
     2941 caggtaaatc aaactcgtgc agtgtatcgc aggctgcggc gatatcatcg gcatgataat
     3001 tcggtttttt gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga
     3061 tggtcatctg accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga
     3121 tagcatccac agcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg
     3181 agagtgccga agccatttca cggtggatct gactttcggt agtacgtgcg ccaatcgcgc
     3241 cccaactgat taaatcagca ataaactgac cggtcaccat atcgaggaac tcagtcgcgg
     3301 ttgggacgcc cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat
     3361 ttacccgata gctgccgttt aaatctggat cggagattag tcctttccag ccgacaacag
     3421 ttcgtggttt ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc
     3481 gcagcgactg cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg
     3541 ggccaataat gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc
     3601 gggagtcggt gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg
     3661 cgggcgttac caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa
     3721 gtctcagaaa tttgttgctt ctacggcagg actgccggga agtgatgcgc atcacgataa
     3781 accaatcgct gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga
     3841 taacaaaaaa ataagctaat gcactagttc tctagtacat tcggcgacta aggcccatga
     3901 tatcgaggat cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga
     3961 tctgattttt acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg
     4021 aggcatagcg actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga
     4081 tagtcaggcc gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat
     4141 ccatatcgtc ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat
     4201 acagactggt gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat
     4261 ccagattgcg caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc
     4321 gcgcatcata tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg
     4381 ttggatccag tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc
     4441 aaaagccttc actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga
     4501 ccagcgggcg cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg
     4561 cacggctctc attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg
     4621 acattactgc gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa
     4681 cgtggcgatc aacagcatta tccattttgt tcttcccgtg atgcagacat aattctatga
     4741 aagcataaat taaaaacgca cagaagcgta gaacgttatg tctggtttat aaaatgaacc
     4801 ttcaatttta ttttttatga aaacagcatt t
//
LOCUS       DAZUGK010000266         4827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZUGK010000266 DAZUGK010000000
VERSION     DAZUGK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.268"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..368)
                     /locus_tag="ACHY5W_004354"
     CDS             complement(<1..368)
                     /locus_tag="ACHY5W_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449113.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELHD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKILALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIA"
     gene            complement(352..633)
                     /locus_tag="ACHY5W_004355"
     CDS             complement(352..633)
                     /locus_tag="ACHY5W_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFS4449114.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            734..1153
                     /locus_tag="ACHY5W_004356"
     CDS             734..1153
                     /locus_tag="ACHY5W_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449115.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1479..1778
                     /locus_tag="ACHY5W_004357"
     CDS             1479..1778
                     /locus_tag="ACHY5W_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449116.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            1851..2069
                     /gene="ydfC"
                     /locus_tag="ACHY5W_004358"
     CDS             1851..2069
                     /gene="ydfC"
                     /locus_tag="ACHY5W_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFS4449117.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            2092..2499
                     /locus_tag="ACHY5W_004359"
     CDS             2092..2499
                     /locus_tag="ACHY5W_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449118.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(2477..2710)
                     /locus_tag="ACHY5W_004360"
     CDS             complement(2477..2710)
                     /locus_tag="ACHY5W_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449119.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            3271..3459
                     /gene="dicB"
                     /locus_tag="ACHY5W_004361"
     CDS             3271..3459
                     /gene="dicB"
                     /locus_tag="ACHY5W_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4449120.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            3456..3644
                     /locus_tag="ACHY5W_004362"
     CDS             3456..3644
                     /locus_tag="ACHY5W_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS4449121.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            3740..>4827
                     /locus_tag="ACHY5W_004363"
     CDS             3740..>4827
                     /locus_tag="ACHY5W_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4449122.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLDEELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDDEGTNQGTANTLSSPVPET"
BASE COUNT         1410 a         1105 c         1113 g         1199 t
ORIGIN      
        1 gcgattgcca ccgcgacaaa atcatcagcg tctctcacca gtcgctcccg cgtctccacc
       61 agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa aggtggcatt
      121 gccttttcga tcgctggtaa caacgccaga attttttcaa ctgcatcagg ggtgtctttc
      181 tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg gctgtcgtca
      241 tgcagttctg ggaacgtcat acccagttca aaataagccc tggttatttc agctgccgga
      301 actttttcac cgtccggatg cgcccaggca ttcatcgcca tgcggatgtg ttcatgcttg
      361 attttcatga atcaagctcc tagaaagtgg ttgtgttaac gttttggtat cttccagctc
      421 gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt caccattact
      481 ggctcgctca ataaggacac ataacgatgc ccctaacact tgacctttac tcaatgcctt
      541 tcttagataa ccgatgctgg taccacactc gcatgcaaac atacgctgtt catctgacga
      601 aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa cacagcaaag
      661 aatacctaca ggtaaacaaa agtcaatacc cacaggttgt ttaccttgca gtaatcgcat
      721 ctattattta cctatggaca aatatgaatt tagacgacag caactcatca aaattcgtga
      781 tgagaaatgc gatggtaaag cggttaacgt ggccagaaag atcgggcgcg agccttctta
      841 tgtatcaaga atgttgtacc cagaggggaa aaagggaaaa aaacggatcg ctgatgatat
      901 ggtggagatt atcgaagagt cctttgggtt accccgggga tggatggatg gtatcgtttc
      961 atcatcaacg aacacagcct ccagttatga aacaagggtt ctaacgccac gacaacgtat
     1021 ttttttagat ctcttagacg aactgccaga aagtgaagcg gataacttat taaaaactct
     1081 tgaagagaaa aaacagtatt acaatatgat ctacgaagaa atccgtaaaa agaaagcaca
     1141 aaacgcatca tagctcacca aacaactagt caccagttaa gacaccgcaa aaagttaccc
     1201 atgggtattt actttttaaa tacctatggg tatccttctt ttcataccaa ctcaccccgc
     1261 tccacagaat gcagggcaat acttcgagtt accaggcagt ggtcaggggt taagtagcca
     1321 gcccgaggcg taagaacatg acggcagggt tcaactttaa taactatgca gcaggttttt
     1381 gttccgctac ccggcgttaa ggggaagcag aggatttctc agtgggcgaa gtcaaacatc
     1441 agaatggaag gcgtccaggg atcagcaaag aaacagcgat ggcgctttat attgatatca
     1501 gcgccattgc cggacaggta agagttatca gagcggtaac taagcggtat gcgcctttac
     1561 ttcagaaagt ctctggtgag tgcaccgaag atattgtcaa cgatttcgtc atcgaactgc
     1621 gaggactcat cttcagttac aaggtgacca caatttttgc agatggctcc cgcgaaactg
     1681 tcagagccct gcggcttaaa ggatgtgtca aagacttagc caccacattc tgggcaagaa
     1741 aacttgattg tattcataac caatttcctc tcgagtaaca gacccctcag aggataccac
     1801 ctcgcctgac gtggttaaaa agcaggcaac gctaaccaca aggagccgac atgcagaaac
     1861 gagaccccgt catcatcgcg ccagactata ccgatgatga actttatgag tggatgcacc
     1921 agaaaattaa ggcggtgcag gacctgaaat gggccaatga agccagggct aagcaggctg
     1981 aaaatctgtc cgctctggag caggatatca ccaatctgga aaaagcagcg gcattaagca
     2041 ttgccagaat gattacatac ccacgttaat agctaaccaa cgaggctaat aatggaattt
     2101 aaagatttac caacgccatt acaggaaatg gcatcgaata tagttcgttc acaactggct
     2161 actcttgacc ttagtaccgc agaaaaagaa accatcgata atatggttcg taatgtgcgc
     2221 aatgcttttt ctgggctata tggttctgat aatcaaaagc aggaaagcga tgttaataaa
     2281 cgggtaattt ctgtttgcgt gaatggccat gttctttcat caatcaaaac agaaacggcg
     2341 acagtcttcg attgcctttg cattgtacag agccttgttg atgccctgtt tcgttcagtg
     2401 aatttagaaa atgatgcaaa tctgcgaggg cgcataatag cacatccata tgcacatact
     2461 ttaggctctg tggatatcaa agatcccaca aatctttaat gaaatagtta acgcgaattg
     2521 tacttgctct ttcggttgct ttcagaatac gcgttgaaac tgctggcggt aatttggtat
     2581 tccatttatt aaaatcatgc ccgggaaagt actcttcgaa aatactttta actgcagact
     2641 cgcctattga aatgctgctt accatgcgat tttgataaag gcatttagca ataagagttg
     2701 attttaacat tcaccctcct gagggttggt aattaaggag ttctccacgg gtgaagtgga
     2761 gtgcgtgcgc cggacacggg tgaacatccg gcactgacag tttactgaaa ggatatttct
     2821 ctgaaaagtc agaccataac gcgaaagcgc acggcgaggt agctggttca tagatagcct
     2881 gtcgttaaat tttcgtcgac cgtgcgcttc cggttgtggc aatccgcgaa atggcgcggc
     2941 ggtaagtatg gaggggttat tccttccccg ttgaggacac cgggttgtca ggttgaccat
     3001 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
     3061 cggtatcagt tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgt
     3121 ggtgaatgcg gctgagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
     3181 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcaccgc
     3241 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
     3301 tctgaaggtt gttttgatat tggtgttctg ctcagtaacc gggagtttac ggaagatgcc
     3361 attaagatga gaaaatatga accttatctt ctcaatgata attccatact ttccagaatt
     3421 gcccttcttg aacttggtat tatcggagaa cagcagtgac ttcagcattt gcactggtga
     3481 tgaccgtttt tcttataacg ggtgagccac aaaatgtgat taccggaatt tatgacagta
     3541 agtcatcctg cattcaggta agggacgaac aaaaaatccc cggtgaatgc ctcccgttaa
     3601 aaaaagtatc gctgaacctg aataacgaaa taccggctgg ataacccgcc agccatatta
     3661 acgccatacc aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct
     3721 gtaatgaggt taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca
     3781 ggcaataaag gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg
     3841 tcgcgcccgc gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg
     3901 cgcttataaa tttcttgtct gcgaggataa acccggtata ccccgccctg ccctcgattc
     3961 ctgggatgct gaatatatgc aggaaaaccg ctgggatgag gaatccgctt cctttattcc
     4021 ggtcgaacca gaatccgatc cgatgaacgt caattttgac aagctgtccc ttgaagtaca
     4081 gaacgcggtc ctggttaagt tcggtacatg tgaaaacatc accgttgata tggcgattga
     4141 cgcgcaggaa ttactgcagg aagacgttgc tacctttgac gggcatatcg ttgaagcact
     4201 gatgaaaacg cctgaaatta acgctatgta tccggaacgc aaactgttcg ctatcggatg
     4261 ggttaaacac aaatgtaatc cgggtgccaa atggcccgaa attcaggctg aattacgtaa
     4321 ctggaaaaaa cggcaggacg cagagcgcaa agagactgga aaatacacgt ctgttgttga
     4381 tctcgccagc gccagagtca atcaacagaa cactgaaaac tcagcaggaa aaatcaaccc
     4441 agtcactgcc gccatttgtc gcgaatacaa gcagacatgg aaaacgctgg atgaagaact
     4501 ggcctacgct ctctggcctg gtgatgtgga tgccggaaac attgacggca gcatccatcg
     4561 ctgggcaaaa aatgaagtta tcgacaacga ccgcgaagac tggaagcgta tctcggcatc
     4621 aatgcgcaaa cagcctgatg cccttcgcta cgaccgccag actatttttg gccttgtccg
     4681 tgaacgtccg atcgacattc acaaagatcc cgtggcactg aacaaataca tcactgaata
     4741 cctgactaca aagggcgtgt ttgaagatga cgaaggaaca aatcagggca cagctaatac
     4801 tctctcgtcg ccagtaccag aaactga
//
LOCUS       DAZUGK010000267         4818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZUGK010000267 DAZUGK010000000
VERSION     DAZUGK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.269"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..360)
                     /locus_tag="ACHY5W_004364"
     CDS             complement(<1..360)
                     /locus_tag="ACHY5W_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFS4449123.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIA"
     gene            complement(627..1292)
                     /locus_tag="ACHY5W_004365"
     CDS             complement(627..1292)
                     /locus_tag="ACHY5W_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFS4449124.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1433..2074)
                     /locus_tag="ACHY5W_004366"
     CDS             complement(1433..2074)
                     /locus_tag="ACHY5W_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFS4449125.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2783..3814
                     /gene="repA"
                     /locus_tag="ACHY5W_004367"
     CDS             2783..3814
                     /gene="repA"
                     /locus_tag="ACHY5W_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFS4449126.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1284 a         1187 c         1289 g         1058 t
ORIGIN      
        1 ggcaatcagt ctgccggtgc ggtgtacgag aggacatgtc agcagcaggc aaagtggtgc
       61 aaggagaatg ttcggggcag gagcctgcag cagatgaata ataagaccct gcaggagaat
      121 gccggatata ccaaaaaaac ctgccagcat acagaggacg atcagcaggg ggatccgtcc
      181 gatgttcagc cagtccagcg cctctcctgt cagcccctcc ggtaaatcag gtgtatccat
      241 agctgaagag agggagtgtc cgcagagcag ggcgataagt tcagcgatac cagtaagcag
      301 aacaaaagtc agggctgcca gatagggtac gttatacgcg gtaagtaacg tcagtgtcat
      361 ttttcctcct gtggcaggtc agtcaaacaa cattatggcg tgaagcttcc gcttctttaa
      421 ttgtcagaag aagcatatcg tcagtcatca gaagacaagg ggatgtctgc aggaggggat
      481 gataacaact gaagcctcac gccggaatgc gctttatgac aaccggagag gacggataat
      541 aaaaaatggg acatccacat cagcaaaccg cgatttgcga aaaaaagtca gcggagagtg
      601 gctgccgttg tggctgtccc ggtatttcag tgtaacaggt gattgctgtg atctatgccc
      661 cattcggctt tcggtaaacc tgaatgcccc acatgaagaa tgcgtgacag catgtttctg
      721 ccgctgccgt tcaggctgca ttcgcgtccg tgcgcgagta taaaaccttc atcccggaat
      781 atctcttcca cttctgaaga ttcgagggat gccggatgaa ttaatgaaaa tgccaccggt
      841 ctgagcattc tgcccgtggt ggaaaaggtg tctctggtac tgttcagctg caggatatga
      901 cgtttacctg tttgcgcacg ggaaagtgtc tcactcagca gctggtcgtt ccatgcgcac
      961 gtgccaatct gccatcccag acttctgcac gcggaaagag ccgagcggaa gcggtacagg
     1021 tcgtataatg ctgcgttatt gccactttcc agcagtgatt tcagcgtatt tgctaccggt
     1081 atgtccgtat tggtggccag cagttttcgc agagtcgtaa actcttctgc cattcctctg
     1141 aatgcgctca tctgacgcgc agattcaagc cagttcagca ggtaacgccg gcgacgccgt
     1201 tcgttgttga tactgtccgg ggcggtggag agtgcttcga gcatattctc cagtaacaga
     1261 atgacagggt tttccaggga tagaatggtc ataaaggcgt cctgaaaaaa tgcccgacag
     1321 ccgctctccg tacaccgtaa ggtgtcaccg gtgccgggca ttgtggaaac agaaggaaaa
     1381 aaaatcccgg ccataaaggc cgggaaaagt cgtcaaatta agacagcaat tttcatgtca
     1441 acgctccgtc atcagatacc aggtgcagga gcagggcgat cctcgactcc ggcagaggcg
     1501 gtgcatcaag ccggaggagg tactggtaac gcgcttttgt cagccagagg gtggaggcag
     1561 catccagctc ctccctggct gccggattga gcgcaggatc cgggtacact ttcatcaaag
     1621 actgaacgat atcctccctg acggttttga tttcaccggc cattttcacg aagttcagaa
     1681 aggcactgtg acggcgaagg tgagtgaagc tgtggcgttc ggggtctgct ttcaggaaaa
     1741 agtaaccggg aaacacggcg tgtatatgtc gacggtaacc gcagacttta tccgtgcggc
     1801 ggactttttt cagtatcagg ggtgtccagg gaaggacgtg ttgttcggaa agccaggaaa
     1861 agagatgttc gcggtttttg ccggcaggga tgtactgagc cagataccag ttgcgttgat
     1921 gtaattctct gatattcaaa atccggaacg ctccgcgaaa atcgatcccc gcagaggggc
     1981 ttcgatgttg ttgtgaacgg cgaatacata aagcatgtgt cggaaacagc ggctgatcct
     2041 cgttcagccc ggcctgcagt tctgacaggc acatgattta cgcactcttg taagccttgt
     2101 ggacggtttc gaggccggcg aaaaccgaca aaacctgcaa acaggattaa tggctggcaa
     2161 acatattaca taaggatata tctgtggcaa gagcgaagat aagtagttaa atagatcgtt
     2221 atatttgata aagcagcata ttacgctggg ttgtgtgagg tgtttggatg attagatcgt
     2281 agatcgtaga tcgtaatcac aagatcgatt catccaaaaa acatgaaaaa cacagcataa
     2341 aagatcgttt gtggaattgt ctattttttg acacagttca aaaaaacgac gttttttagg
     2401 tggttaatga gaggcctggt aaatcagaga aatcgtgttg gcttttcaaa gcggtggaaa
     2461 aggggtatat tgcggatcgt tatgcagtgg cttttgggat cgtcaggatc cgggaagtca
     2521 gaaaatggca ggatgcgcca taaggcattc aggacgtatg gcagaaacga cggcagtttg
     2581 ccggtgccgg aaggctgaaa aaagtttcag aaggccataa aaggaaaacc cccataatct
     2641 tctcatcgaa cttggcggaa cgttagaaga ttatgggggc ctcacagaat acgggataag
     2701 tatatatgaa accgtgtcag agattcaacc cttcgcagtg tattaacgca cggcattctg
     2761 tttccgccat aagcgacagc ttgtggcagg tctgaagaat actccatata acgcagtaca
     2821 ctggagtcag ttagcacccg aagagcagat ccgtttctgg gaagactatg aagcgggaag
     2881 ggcgacctct ttcctggttg aaccggaaag gaagcgcacc aggcgccgtc gtggcgaaca
     2941 ctccactaaa cccaaatgcg aaaatccgtc ctggtatcgt cctgcgcgct ataaggagct
     3001 gagcgggcag ctcgggcacg cctacaaccg tctggtgaaa aaggccccgg tgaccggcga
     3061 gcagagcctg cgtatgcgta tgtcgcggca tcctttttac gtgcagaaac ggacgttcgt
     3121 cggtcgcaaa tatgctttcc ggccggaaaa acaacgcctc ctcgatgcca tctggccggt
     3181 tctggtcagc ttcagtgatg ccggcacaca cactgtgggg atgagtgtca ctcgcctggc
     3241 taaagaaatc agtccgaaag acagtaaggg aaatgttatt ccggaactgg aagtgacggt
     3301 ctcccgtctt tcccgtctgc tggccgaaca ggtgcgtttt ggtgtgctgg gtgtttcaga
     3361 ggaaacgatg tgggaccgtg aaacccgcca gcgtctgccg cgctatgtct ggataacacc
     3421 ggctggctgg cagatgctgg gcgtcgacat ggtaaaactt cacgaacagc agcagaaacg
     3481 actgcgtgaa agtgaaatcc gccagcagct cattcgggaa ggtgttctgc gtgaggatga
     3541 agatatctcc gtacatgcgg ccagaaaacg ctggtatctg cagcgcagtc aggatgcgct
     3601 gaaacaccgt cgtgcgaaag cagcagccag taagcgcgcc agacgcctga agaaactgcc
     3661 tgccgaccag cagattcatg agatggcaga gtatctcagg aagcgtctgc cgccggatga
     3721 agcctatttt tgttccgatg accatctgaa gcgactggcc atcagggagt tgcgtcagct
     3781 tgaactgacg ctggctgccc cgccaccgca ctagacagca ccattccctc agcactgaat
     3841 catcgccagc ccctccgggg ctttcggcgc aggtatcgct cagcccaaaa tccgcagtaa
     3901 tcaccttaaa tcccctcaga ggggcatatt tgctcataaa accacgcatc agtcatcaga
     3961 acgtggccac gttgtttcag ttatccacat aaatccgcaa acaaagaact ttaagaagct
     4021 gcaaacctga aacagcaaac ctgcaatata gtcttaaccc cattatttaa tcccctgcgt
     4081 tgcttcgccg cagcgaaagt ctttatctct gaaaccactg tgaacaaaca caaagaggcc
     4141 ttcgcttgca gcggccaagg ccgcgccgct cagaatctaa aagcacctcc cacgctgacg
     4201 cgcgggcccc gaacctcacc gttctggaac cacaacaaaa aaacatcagg aataaaaaca
     4261 ccacacaaac gcagcaccgt gcccacccct cataactgaa aagcgccgcc gccccggccc
     4321 aaagggccgg aacagagtcg cttttaatta tgaatgttgt aactaaacat catcatcgct
     4381 ttaagacccc atttgtctag cagggcgatg gcccactgcg tgaaccatca ccctgcacac
     4441 agggtatttt atgtgcaatt gtcaaccact gaatcggaac cccagagggg cccccacgat
     4501 tcagaagttg acaatttaag cacagggact acatgagaaa aatcgtggtt cgtctgtcat
     4561 acacgcgcgt aagcggcact gatagccctc ttacgcggaa atacgcccat aagcgctaac
     4621 ttaagggttg aaccgacgcc acggtgtctc gttaagactt caattgcgtt ttgtaggcag
     4681 tcagggatac tgtcccatga atggccacct gtaagctcca gataaccatt tttgttattc
     4741 tccacaacga attccgcata ccggaaatca gcgctacttc tggggaggaa tggccttaag
     4801 agtgataaag attatatg
//
LOCUS       DAZUGK010000268         4805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZUGK010000268 DAZUGK010000000
VERSION     DAZUGK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.270"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1849
                     /locus_tag="ACHY5W_004368"
     CDS             <1..1849
                     /locus_tag="ACHY5W_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS4449127.1"
                     /translation="NNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDE
                     RMLPDSMRGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLD
                     VTVTEANGTVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTN
                     TLTFNSGSRIADGYQALMLGGVYGSTLGAFGANLTWSHARVPESEAQSGWMSQLTWSK
                     TFQPTSTTVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQS
                     LANYGNLFVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSND
                     MQTVTSLSFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVM
                     RDQQYKQTTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLG
                     ETFALVEAKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDSDAELVD
                     SEKQVAPVAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGG
                     QIYLRTEQTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            1865..3199
                     /locus_tag="ACHY5W_004369"
     CDS             1865..3199
                     /locus_tag="ACHY5W_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449128.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDGSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYIFNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            3196..3885
                     /locus_tag="ACHY5W_004370"
     CDS             3196..3885
                     /locus_tag="ACHY5W_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4449129.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRMIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPEEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGIVTATYLDD
                     NGAQIKQQLAQ"
     gene            complement(4031..>4805)
                     /locus_tag="ACHY5W_004371"
     CDS             complement(4031..>4805)
                     /locus_tag="ACHY5W_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4449130.1"
                     /translation="SIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANLLLV
                     DIKEPDNRYLKHLTHEGVEVEFMTIDITKPDAACTIINRCLERFGQLDILVNNAGVCN
                     INRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWSP
                     GYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIP
                     AQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1300 a         1177 c         1149 g         1179 t
ORIGIN      
        1 gaacaatatt cgtagctatt tgcaacgccc gctgcccgcc ataaatagcc agttaatgat
       61 gggacagctt atcaccagcg gaagattttt ctctggactc agttatcacg gcgttagtct
      121 cgcgaccgat gaacgtatgc tgccggactc catgcgcggc tatgcgccga ctattcgcgg
      181 cgtggccgca acaaacgcca gagtctcggt aatgcaaaac ggtcatgaaa tatatcagac
      241 caccgtggct cctggccctt tcgagataaa cgacctatac cccaccagtt acagcggcga
      301 tctggatgtc accgttacgg aagcaaatgg cacagtcagt cgtttcagtg tccccttttc
      361 agccgtacca gaatcgatgc gtccaggaac ttcccgttat aacgtggaag taggtaaaac
      421 gcaggatagc ggtgatgact cgatgtttgg tgacctcacc tggcagcacg ggatgactaa
      481 tacgctgaca tttaacagtg gctcgcgtat cgctgatggc taccaggcgc tgatgcttgg
      541 cggagtctat ggcagtacgc tgggggcatt tggggcaaac ctcacttggt cccatgcgcg
      601 tgttcccgaa agcgaagcac agagtggttg gatgtcgcaa ttaacctgga gtaaaacttt
      661 ccagcccacg tcaaccactg tctctctggc aggttatcga tactctacca gcggctatcg
      721 tgatctggct gatgtgctgg gagagcgtca tgctgccagc aataaacagt catgggactc
      781 cagccaatgg cgtcaacagt cgcgcttcga tcttacgtta agtcagagcc ttgcgaatta
      841 cggcaacctg tttgtgtcag gttcaacaca gaactaccgt ggcggtaaga gccgtgatac
      901 acagcttcag ttaggttaca gcaatagctt tagccatggc atcagtatga acctttccgt
      961 cggacgccaa agaatgggcg gctataaaga caattctaat gatatgcaga cggtaacgtc
     1021 cctttcattc tcattcccac ttggcggcaa tggacctcgt gtaccaagtc ttagcaacag
     1081 ctggacccat tcaactgacg gcagctcgca attacaaagc tcgctaaccg gaatgcttga
     1141 tgaagcacag accaccaact acagcctgaa cgtcatgcgc gatcaacaat ataagcagac
     1201 gacgcttagc ggaaacatgc aaaaacgttt ttcacaaact accgtcggat tgaacgcatc
     1261 gaagggccag gattactggc aggcttccgg aaacgtacaa ggtgcgatgg ccgtacatgg
     1321 tggcggcgtt actttcggac cttatctggg tgaaacgttc gccctggtcg aagctaaagg
     1381 cgctgaaggt gcaaaagtct ataactccag tcagctggaa attaatgaca gtggttatgc
     1441 gcttgttcca gcagtaacgc cctatcgcta caaccgtata tctcttgatc cacaaggaat
     1501 ggatagcgat gccgagctgg tcgacagtga aaaacaggta gcaccggttg caggtgcggc
     1561 ggtgaaagta gttttccgta cccgtcctgg taaagcgttg ctgattaaat cccgcatggc
     1621 agatggttcg gaattgccaa tgggagccga tgtgctggat gagaataata cagtcgtcgg
     1681 tatagccggt cagggggggc aaatttacct ccgcacagaa cagacaaaag gccatttgtc
     1741 agttcgctgg ggtgaaggcg ctaacgatag ctgccaattg ccctttgata tcagcgggaa
     1801 ggacagcaat agccctatca tccgcctgaa tgaaacctgt cagtcttgat acagagaaaa
     1861 cccgatgtct aaatcgactt ttttacacat tctgattagc tccatcatat tggtggcact
     1921 aatacaatca tccgcctggg ctaactgtac aaacacacag ataggtcaaa ctgaagatgg
     1981 tcggacagcg cttattgagt tcggaaaaat taatatgacc gacacctatt ttgcgccagc
     2041 aggttcactc ctcgcgacaa cggtcgtacc tcccactaac tacacatcag gcggcgcgac
     2101 aggaagctcc gtattgtggg aatgtgatgc aacagatttg ccaaacatct attttctggt
     2161 cgctaccaat ggcgacgacc gcgtgggggg cttttatgat gcaggcgggc ctgatggttt
     2221 gagtgatgtc tatgccacct ggttcgcttt tgtcggtctc aagcagacca tggcgggcgt
     2281 gacgcttggt cgttactgga agaaagtgcc catcaccagt tatgccactc agggaactaa
     2341 aatccagatc cgcttacagg atatccctcc tcttcatgct gagctttatc gcatcagtac
     2401 gctacctgat acatcagcaa caacaagttg gtgcggtaat aataatacgg atggtagtgg
     2461 agtcggattc gcaaaacctt ccggtacaat ctataactgt gttcagccca atgcctatat
     2521 tcagctttcc ggtaccagcg gcattttatt tggtcatgat gagcccggtg aagatagttc
     2581 tgttcattgg gatttctggg gggctgataa tggttttggt tacggaatgc gttcggccaa
     2641 tcgactctac aacaatgcca cctgcgttgc ccgcagcgcc acgccgttag tattgctgcc
     2701 gacaattgca gaagcacaac tgaatgcggg catggaaagt accggtaatt ttaatgtccg
     2761 cgtcgagtgc agtaactcag ttcaatcagg gattagcgat actcagacag cattaggaat
     2821 ccaggtgtct gaaggtgcat atacagcggc gcaaaaactg gggattatca atagcaacgg
     2881 cggcgtcagc gccctggtct ctgataatta tgacgcagca gagatggcaa agggcgttgg
     2941 gatctacatt tttaacagtg ctcaccccga tacggcgatg acgctggttg gtcaaccggg
     3001 catcgcgaag ttaacccccg gaggaaatgc agcggggtgg tatcctgtat ttgaaggggc
     3061 aacattagaa ggtgcgactc accccggata ctccagctat agttactctt ttatcgcccg
     3121 gttgaagaaa ctgccaaatc agacagtcag tgcgggaaaa gtgcgggcaa cggcgtatat
     3181 attggtgaaa atgcaatgaa atccgttttg ctgctcttaa ccgtcttgtt cgtacagtca
     3241 gtggaagccg gcatcatgcc ggcttcttca cggatgattt atcgcgatgg cgatcgggaa
     3301 aagacactga tgctggtcaa taccaatgac tacccggtac tggtgcaaag ttgggtagat
     3361 aatggtgaag gttccccaga agctgcttct cccccctttg tggtgctgcc accggttttc
     3421 cgcctggctg cgagacgaac acagggattg cggataatct ataatcatga cccacttccc
     3481 caggatcgcg aatctgcatt ctggttaaat ctttatgaaa tgccgccaga aactccccat
     3541 caagatccca cacatttaac gctggcaatg aatacgcaac ttaaaatctt ttacaggcct
     3601 aaatctgtaa ctatgacgcc tgaagaagct atgcgtaaaa taagttggca tctgggtaac
     3661 gacggcagca gtagctatat tgagcttaat aacccaacac cactgaatat ctctttcaca
     3721 tccatcagca ttggggctac gaaggtacag caagaaagcg atatgatgat aaaacccttc
     3781 agtcaacgac ggtactggtt agcaagtaat aacgttagtg ggatcgttac tgcgacatat
     3841 ctcgatgata atggtgcaca gataaagcaa caattagcgc aataatgctc tgagtggtat
     3901 gtaaactcat cgcgatggtt caacgctaac gaataaccac tatacccgcc tgaagtaccg
     3961 gaagtggaga aatgacaaaa tctctccact ccattttcca gtcaaaacac ctcaacgcag
     4021 ccaaaaatcg ttaacgaatt gaatatccac catcaatgac taatagttgt ccattgacat
     4081 aatctgaagc ggcactggcg aggaacacca ttgcgcccat aagatcctgt gcccttcccc
     4141 agcgttgagc cggtatacgc ccttttatta actcttttat tttagggtta ttataaataa
     4201 tagcggtcat ttcactaaca taatatcctg gggcgatacc attaatctga atattatatt
     4261 ccgcaaaatc atcggcatac gctttggtca gccccgctaa agcatgttta gtcgccgcat
     4321 accctggcga ccaacgtccc ccatgaaatg acaatacgga acacatatta ataattttcc
     4381 cttttctttg cgggacaaat atattcaatg cggcctggct catatcgaat gcagcattca
     4441 ggttgagatt aatcatagga tcccagtcgt ttcgattgaa atcgatagga cgattgatgt
     4501 tacacacgcc cgcgttattg accagaatat ctaactgacc gaaacgctcc agacacctat
     4561 taataatggt gcatgctgca tcaggttttg ttatgtctat tgtcataaac tcaacttcaa
     4621 cgccttcatg agttaaatgc tttaaatatc tattatcagg ctctttgatg tctaccaaca
     4681 acagattagc ccctgctttg gccagagccg agcataagcc tctccccagt tcacccgcgg
     4741 ccccagttac gatggccacc ttgttatgta atgaaaaaaa atctaatgaa aaatcttcaa
     4801 tagaa
//
LOCUS       DAZUGK010000269         4776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZUGK010000269 DAZUGK010000000
VERSION     DAZUGK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.271"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..606
                     /gene="ipbA"
                     /locus_tag="ACHY5W_004372"
                     /gene_synonym="fimX"
     CDS             85..606
                     /gene="ipbA"
                     /locus_tag="ACHY5W_004372"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFS4449131.1"
                     /translation="MDAAQSMSFPERNRCLIMMAFIHGFRASELLDLRLSDIDASGKQ
                     LNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLFLSRKRRPISRQHFF
                     SIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLGHRNIQHTVRYTASN
                     AARFKGVWKKKPR"
     gene            936..1730
                     /gene="hyxR"
                     /locus_tag="ACHY5W_004373"
     CDS             936..1730
                     /gene="hyxR"
                     /locus_tag="ACHY5W_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFS4449132.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYLVRVYSNVTTEVEFILK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYYGFP
                     RISTLLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1884..2645
                     /gene="hyxA"
                     /locus_tag="ACHY5W_004374"
     CDS             1884..2645
                     /gene="hyxA"
                     /locus_tag="ACHY5W_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFS4449133.1"
                     /translation="MRFVLFCPSGIVPAQFVALSTGSVGNVTCIKTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANTSGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            2687..4627
                     /locus_tag="ACHY5W_004375"
     CDS             2687..4627
                     /locus_tag="ACHY5W_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4449134.1"
                     /translation="MKNNKAFYRSALATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LDQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLNIKDGSVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENT
                     LDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADG
                     NFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYF
                     GGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDT
                     EGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKG
                     GYTWKDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDFAVG
                     NASVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASL
                     DYTKGDDIENPLQGVVGINVTW"
BASE COUNT         1342 a         1029 c         1149 g         1256 t
ORIGIN      
        1 aggcatactt atctgggtgt gcctctcttt tttacaactg tacacggtgg cgtatatctc
       61 acacaggatg aagtctacag gctgatggat gcagcccaga gcatgtcctt tcctgaaaga
      121 aatcgctgtc taattatgat ggctttcatt cacggtttta gggccagtga acttcttgat
      181 ttacgtttat ccgatattga tgcatcagga aaacaactta atattcggcg gattaaaaat
      241 ggtttctcga caacacatcc tcttcttcct gatgaatata atttgatcaa gttgtggctt
      301 aagcagagga agttaattga aaacggagtt gaaggagact ggctttttct ctcccggaaa
      361 cgccgcccta tcagcagaca acattttttt tctatcattc gtgaggctgg aagacgtgca
      421 ggattagctg taaaagcaca tcctcatatg ttacgtcatg cctgtggttt tgctctggct
      481 gacaatggtg tcgatacccg actacttcag gattatctgg gtcatcgaaa tatccagcat
      541 actgtcagat acacagccag taatgctgct cgatttaaag gggtgtggaa aaaaaagcct
      601 cgttaaaaca gtaccttctg attcaaaatg tcagattcct acgttttcct caattcttcc
      661 ataaaaaaac agcccattca attcaataaa acacataaca tgttgatatt tctcgctaaa
      721 gtaaaataac cccatgattt gaaatgatta accgccaagg tcataccagt gattaacttt
      781 cactcatgtt gttaaatgca accaaattaa aaaaaataaa aatcaaaaaa aatagattca
      841 tattccaaga aaattaaata ttcaggcaat taaaccaaac ctgacatttt gaatcaaaat
      901 gtcaggtttc gtagcaatgg cgtggacgag tctgaatgaa gaagcctctg gttattatca
      961 gtgcctgtca gttcaccagg ctggcactgg agtctctcat cccaacggac aggtatctcg
     1021 tgcgggtgta cagcaacgtc actacagagg ttgaattcat tttaaagacc tcctgtggct
     1081 acctgttagc tgatatccct tcgcttcctt cgggggagtt ggttcttctg gcttgtcttg
     1141 tccgcttgcg gagtgatgcc gggcaatggc aggtctgcct gaccggtgac tccgactggt
     1201 tcgataatgc tcctgctgca agtctgtact atggcttccc acgcataagc accctgctga
     1261 cagcgagtcg ttatcagaat caaattatcc gctggttact acgtcctttg cccggcgata
     1321 tgagtgattt ctggctgctg actgcacggg agttgagcgt gcttaagatt ctgatgcagg
     1381 gcatgagttt cagtgagatt gcccgttatg aacaacgcag ttcaaagacc ctgcatgcga
     1441 ttgccacacg ggcattaatg aaacttggac tgggaactct gagcgatttc aggctgctct
     1501 atacgggatg cggtgacagc agagtgaaac ggaatatacg gctacatgca agatatgtcg
     1561 gacaacattc cgctcatcga ctcactcagt atgtgcagaa catggtatcc ggaattcttt
     1621 atccagagaa agttgccgtt ggtgaatcaa taaattctga cgccatatcc ggaaaagtac
     1681 tttccggaca aagaaaaaat atgtctaaaa aaagaccagg attgttatga cacagcagcg
     1741 ttgttactac cccccactgc ggcgcatacg gactcgctac agtgtaggag ccaaaatatt
     1801 tctactgctc tgggctgcag gattgggagg cacgctgtac agcctgtaca gcgtgctggc
     1861 gtactgacat ctgtgaggga aggatgcgtt tcgttctttt ctgtccctca ggaatcgtac
     1921 cagcgcagtt cgtagctcta tcaaccggtt ctgtcggcaa tgttacctgc atcaaaacag
     1981 aagaggagtt acgcaataaa ttgcggcaca ggccacagag tgtcgtgata tcggcagggc
     2041 gacctgccga atgcgctgaa atgtggttcc gattttatcg ggatcactca ttcgtggtgg
     2101 tgttgtgcgt cgcgcccttt tttctgccac cggatgtgag tatttctggc gttctcaaaa
     2161 acctgaggtt actgaaacca ggaatgtcag tagagcatgt catcagtatt gcaaatacct
     2221 ctggtgggtt ttcagggctg aaacatgcag aaattttgcc agttatggac agttactctg
     2281 tctttatgaa agaggtgaat aaccgcacaa agacaatcgt catgtcagaa cgcttcccgg
     2341 aaaagcaaaa gaaggtgttg tcgttgcttc ttgccggaca cagctgggaa tacagtgcgc
     2401 agtttctcaa aacggggata agacagatat ggcttgccga gcagtcgcta aaaaagcgtt
     2461 ggggaatacc ggacagcatg tcattgcggg aagccctgct gctgagcagc aataaatttc
     2521 acggtgatgg aaatgcattg gaaaaaacga atgccatgac attgcgtgaa aacggaaata
     2581 ccaacaggta ttccgtggtg gtaaatgcag ggacgcaggc tttatctctg cataaatata
     2641 aataagtggg aaattgaaat tagttaccag aatggagaat ttcttcatga aaaacaataa
     2701 ggcattttac cgcagcgcat tagcgacagc tattgttatg gctctttctg caccagcatt
     2761 cgctgctgat aacgcggtat caactacacc ggttacattg gataaagata agacaactct
     2821 ggatcaagat gttgttatta acggtgataa aaagattaca gccgtaacaa ttgaaacgtc
     2881 agattcagat aaagacctta atgttacttt tggtggtcat gatattaccg ccgcatcaac
     2941 ggtaaaccaa gatttcgttg aaggtgtaaa agttagtggt aacaaaaatg ttgtgattaa
     3001 tgctacagac tccaccatca cagctcaagg tgaaggcacc tatgtccgga ctgcaatggt
     3061 cattgattca actggcgatg ttgttgttaa tggcggtaat ttcgttgcaa aaaatgaaaa
     3121 aggtagtgcg acagggatat ctctggaagg ggcccaggga aataatgtca cgcttaatgg
     3181 cacaaccata aatgctcaag gtaataagag ttccagcaac gcctccacgg caatttttgc
     3241 tcaaaagggt agcgtattga atggttttaa cggtgatgca acagacaaca ttacccttgc
     3301 tggctcaaat attattaatg gtcggattga aacaatactt attgctcagg agaataaggg
     3361 aactcataca gtcaacctga atattaagga tggctctgtt gatgctctgg cagtaaaaga
     3421 cagtacggtt aatatcacga aggctacagt caatactggc acgtttgctt ctcaaaacgg
     3481 cactctgatt gttgatgcct cttctgaaaa cactctggat atcagcggta aagcgagcgg
     3541 tgacttgcgt gtttacagtg cgggttcatt ggatcttatc aatgaacaaa cggcatttat
     3601 ttctaccggc aaagacagca ctctaaaagc cacaggcaca acggaaggtg gtctgtatca
     3661 atatgacctg acacagggag ctgatggtaa cttttatttc gtaaaaaaca cgcataaagc
     3721 atccaacgcc agctccgtga ttcaggcaat ggcagctgct ccggctaacg tcgctaatct
     3781 gcaggctgac acgctctcag cccgtcagga tgctgtccgt ctgagcgaaa atgacaaggg
     3841 cggcgtatgg attcagtact ttggcggtaa acagaaacat accaccgcgg gaaatgcatc
     3901 ctatgacctg gatgtaaacg gtgtaatgct gggtggtgat acccgcttca tgactgaaga
     3961 tggtagctgg ctggccggtg tggcgatgtc ttctgcgaaa ggtgacatga ccaccatgca
     4021 gagcaaaggc gacactgaag gttacagctt ccacgcttac ctgagccgcc agtataacaa
     4081 cggtatcttc attgatactg ctgcacagtt tggtcactac agcaacacgg cagatgttcg
     4141 cctgatgaat ggtggcggta ccatcaaagc tgactttaac accaatggtt tcggtgcgat
     4201 ggttaaaggc ggttacacat ggaaagacgg taatggcctg tttattcagc catatgccaa
     4261 actgtctgcg ctgacgctgg aaggtgtgga ttatcaactc aacggcgtga acgttcattc
     4321 tgacagctat aactctgtgc tgggtgaggc cggtacgcgc gtgggttatg acttcgctgt
     4381 gggcaacgcg agcgttaaac cttatctgaa tctggccgca ctgaacgaat tctctgatgg
     4441 caacaaagtc cgtctgggtg atgagtctgt caatgccagc attgacggtg cagcattccg
     4501 cgtgggtgca ggtgtacagg ctgatatcac caaaaacatg ggagcatatg caagccttga
     4561 ctacaccaaa ggtgacgaca ttgagaatcc gctacagggt gtagttggta tcaatgtgac
     4621 ctggtaagaa taaagcagtc ggttcccctg tcgactgtta taccccctgc aggcagcagt
     4681 atgctgcctg caggctataa atatccagta ttcatgatgc tggctttttg ctgatattta
     4741 acccgaacat tcctgataca gactattaat tcattt
//
LOCUS       DAZUGK010000270         4769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZUGK010000270 DAZUGK010000000
VERSION     DAZUGK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.272"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACHY5W_004376"
     CDS             complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACHY5W_004376"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFS4449135.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTVLGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACHY5W_004377"
     CDS             complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACHY5W_004377"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4449136.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1980..3161)
                     /locus_tag="ACHY5W_004378"
     CDS             complement(1980..3161)
                     /locus_tag="ACHY5W_004378"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS4449137.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3416..4645
                     /locus_tag="ACHY5W_004379"
     CDS             3416..4645
                     /locus_tag="ACHY5W_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4449138.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1240 a         1217 c         1044 g         1268 t
ORIGIN      
        1 tatgatgatc tacaggtata aaaaaccctg ccatgcggca gggtcataaa agtaagaaga
       61 atgaattaac gcgccagcca accgccatcc acggcaatgg tataaccatt cacataatct
      121 gaagcgctgg aggcaaggaa cactaccggc cccatcaggt cactcggcag tccccaacgg
      181 ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga
      241 gtattgttgg ttgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
      301 cattcgttcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa
      361 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
      421 ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
      481 ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcatc ttcgcggcga
      541 atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc
      601 agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
      661 cccagcaccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
      721 tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagtccagt atcacaacca
      781 gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat
      841 tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
      901 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
      961 ggatcgacca gctcggggag atcaccgcct gctcgttatg cattacaata tgacgcgttt
     1021 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     1081 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
     1141 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     1201 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     1261 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1321 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
     1381 acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     1441 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     1501 taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaacaataa
     1561 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     1621 aaaattcatt gcgcagccct tgggtatcca gtgtttttgc gtgcgcactg tggatgctct
     1681 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     1741 aaacactgtt tcgttttctc tataagggga tgatatgccc atgggatagg gttttcaatt
     1801 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
     1861 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatttcg acttgccagc
     1921 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     1981 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
     2041 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2101 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
     2161 tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2221 tcgataagat cgacatcagc caactgccag cccacacgct ccaggcaacg gcgggtcgca
     2281 tacaccggcg caattcccat caatgctgga tctacaccaa cgctggcaaa tgcgcggatg
     2341 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
     2401 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
     2461 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
     2521 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
     2581 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2641 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2701 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
     2761 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2821 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagttgc cagatgcagt
     2881 gccttaagcc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
     2941 ccacctttga tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctgg
     3001 ccaagaataa cttcatccac cgcatatgca ggcacgccgg tacgttctat taacgctttc
     3061 acgaccaggc taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
     3121 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcacaaatta
     3181 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgact
     3241 tttatcacaa aggaaatacg cctgaccagc agtcagagac ataactggca cgtaacgttt
     3301 gcaaccacta acccaccaat agaggggtag atagggcgtt aatctcccat acttaacctg
     3361 gtttatggta aattgccctc tattttgttt aatttgtaga tgatacgttc agataatgtc
     3421 taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     3481 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
     3541 tactgctctg gtcgcctggc ctttaacata ctggccacat aaagccttat gtcagttcat
     3601 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcagtaacac actactatgg
     3661 caagaagata ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3721 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggtgat
     3781 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaatcctga atctcatttt
     3841 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
     3901 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacaac
     3961 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4021 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4081 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4141 tataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
     4201 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4261 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4321 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cattacagca
     4381 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     4441 gatcaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
     4501 cggcccactc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
     4561 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     4621 cgtatcggta atgttcttta gctaatatcc attgtgaatg gttcaaaatt gctacaattt
     4681 tgaaccattc catttttcat aatgttcatt tgttattatt ccgccctatc acttctctca
     4741 tcatatccta gaattatcag ttagttaaa
//
LOCUS       DAZUGK010000271         4745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZUGK010000271 DAZUGK010000000
VERSION     DAZUGK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.273"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..801
                     /gene="paa"
                     /locus_tag="ACHY5W_004380"
     CDS             43..801
                     /gene="paa"
                     /locus_tag="ACHY5W_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFS4449139.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1137..1292
                     /gene="hokD"
                     /locus_tag="ACHY5W_004381"
     CDS             1137..1292
                     /gene="hokD"
                     /locus_tag="ACHY5W_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4449140.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1513..1770
                     /locus_tag="ACHY5W_004382"
     CDS             1513..1770
                     /locus_tag="ACHY5W_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449141.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1840..2118
                     /locus_tag="ACHY5W_004383"
     CDS             1840..2118
                     /locus_tag="ACHY5W_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449142.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2120..3175
                     /locus_tag="ACHY5W_004384"
     CDS             2120..3175
                     /locus_tag="ACHY5W_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4449143.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3176..3541
                     /locus_tag="ACHY5W_004385"
     CDS             3176..3541
                     /locus_tag="ACHY5W_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4449144.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3538..4227
                     /locus_tag="ACHY5W_004386"
     CDS             3538..4227
                     /locus_tag="ACHY5W_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4449145.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4423..4498
                     /locus_tag="ACHY5W_004387"
     tRNA            4423..4498
                     /locus_tag="ACHY5W_004387"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4456..4458,aa:Ile,seq:cat)
     gene            4589..4662
                     /locus_tag="ACHY5W_004388"
     tRNA            4589..4662
                     /locus_tag="ACHY5W_004388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4623..4625,aa:Arg,seq:tct)
BASE COUNT         1261 a         1078 c         1257 g         1149 t
ORIGIN      
        1 tttataactt tatttcatga taaccaataa aaggagtttt ttatgaggaa cataatggca
       61 ggttttttaa tattcctgtc ttctgctgct tatgctgata tcaatctgta tggtcctggt
      121 ggcccgcata cagccttgct tgatgcagcc aaactttatg ccgaaaaaac aggtattata
      181 gtgaacgttc attacggccc acagaacaaa tggaatgaag atgccaaaaa aaatgcagat
      241 atcttgtttg gcgcatcaga acaatctgct ctggctatca ttcgggacca taaagacagc
      301 ttcagtgaaa aagatattca gcctctttat ctgcgaaaaa gtattttact ggtaaagaaa
      361 ggtaatccta aaaatatccg gagtattgac gacctgacca gacctgggat tggcgtaatt
      421 gttaatgatg gtggtggtac cagtaataca tcaggcactg gcgtctggga agatattgcc
      481 ggacgtaaag ggaatataga aactgtcgcc gcaatccgaa aaaatattat tttatatgcg
      541 cccaatagcg gaactgcacg taaggctctt gagaatcagc ctggagcaga tgtctggata
      601 acctgggctg actgggcagc cagtaatcca gaaattggtg atgtcgtgga aatagcgcca
      661 gactacgtga tatggcgtga tatgaacatt acagtacgtc aggatgcaaa tgatgaaacc
      721 cgtcgatttg cagaatggct acaaaccgat gaagcggcgc ctgcattcaa aaaatatggc
      781 tggaccagga aaggcacttg acatcctcgt ccttcaggac gtggattctt tttccggatg
      841 ccgcgccagc ggcatgtagg ggcagctcac aaaacggaaa aaattgtacg ctaagcctcg
      901 ccaggtgaac tgaattcatt ccgatatggg aattcccata tcgggcgaaa acggtttgct
      961 gtaacggcag agttaagtag gattgctgcg ggtgcttgag gctatctgcc tcgggcatga
     1021 acaccaacgg cagatagata aaagccccac ccgactataa atcgaagtga ggcccctata
     1081 tgctcgtcac atatagattg cctcttacgg accgaaaggt caaggagaag caggctatga
     1141 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     1201 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     1261 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     1321 cacctctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     1381 tgtttttatt ttcaacgcgt ttgaagttcc ggacggcgcc ggaatagaat caaaaatact
     1441 taagtagcgc gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga
     1501 aggattctgt tgatgagaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat
     1561 actgtgcgcg ttgagaaggg cgctattgtc agccactgcc gtattaaatg tttacagtct
     1621 caacaaagga agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt
     1681 tttattttgc gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt
     1741 aaggctggtt tttctgcttt tgaagattag tgaattcatc tacaattcag cgcagggctg
     1801 aacccctgtt gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta
     1861 cacaattctg ataattcagc cgtctttgcc agcaggcacg ggcggcgttc tcatgcattc
     1921 aaatctgact ggttccggca cgccccatgc actgaagaac aggccgaatg gctgattcag
     1981 aactaccgca gacgtgggta tgagtttagg aaagccctca gcctcgatta tcgtcactgg
     2041 ataatctacg tcaggctgcc gtactccgag cgcccaccgc gtccgtcccg cacattccag
     2101 caacgcatct ggaggtaacg tgcgggtatt acttcgacct gttccggtac cggaacttgg
     2161 gttggtggtc cttaaaccag gccgtgaatc catgcaggta ttccataacc ctcgagtgct
     2221 tgtggagccg gaaccgaaaa gcatgcgcgg tctgccgtcc ggcatcgtcc ctgccgttcg
     2281 ccagccgctg gcggaggata aatcattact gccgtttttc agcaatgagc gtgtgattcg
     2341 tgctgttggt ggcgctggtg cactgtctga ctggctgttg cgtcatgtca aatcctgcca
     2401 gtggcctcat ggtgactatc atcacagtga aaccgttatt caccgttatg gtaccggcgc
     2461 aatggtgttg tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga
     2521 gcaacttgct catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag
     2581 cggtacgcag gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca
     2641 ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa
     2701 aatccgctca atgtaccgtg aaagcgacat cgtaccggga gagcagaccg ccaccatcat
     2761 actgaagcag cgcacaaaaa atcttgcgcc gctgcctcac gcccaccagc aaaacccgcc
     2821 acaggaaaag acggtggtca gcattgccgt tgatccggag tcaccggctc agtatctcca
     2881 gcgccagaaa ccacaacggg aagagatgcc tgtatacacg cgctgggtaa aaacgcagaa
     2941 atgcatgacg tgcggtaatc aggcagatga tccgcatcac atcattggtc atggactggg
     3001 agggatggga acaaaggctg atgatttgtt tgttattccg ctgtgccgta aatgtcataa
     3061 cgaactgcac gctggggtaa aagattttga agaaaaacac ggcagccagc tgttgttgct
     3121 gattcgtttt ttaatgcacg cgagaaattc gggtgtcctg aagtggaaag catgaatgac
     3181 tgaacggata gaatttgttt tgccttaccc gccgacggtg aatacctact ggcgacgtca
     3241 tggcaatacg tatttcatct cggaagccgg aaagcgttat cgccgtgatg tggcgctaat
     3301 tgttcgccag cagcggctga aattaaacct gtccggaagg ctggtgataa agattattgc
     3361 agagccaccg gataagcgcc gtcgtgacct ggacaatatc ctgaaagcac cactggatgt
     3421 gctgacgcat gccggacttc tcatagacga cgagcagttt gatgaaatca atattgtgcg
     3481 cggtcagctc gttcctggtg ggcggctggg gataaaaatc acagaactgg agtgcgcatg
     3541 aataaccact atttacagtt tgtgcgtgag cagctcatta tcgccaccgc tgatttgagt
     3601 ggggcaacaa aaggtcagct tgaagcctgg caggagaatg ccatgttcga tacagggcgt
     3661 tacaggcgaa aaaaaatccg gtaccgcgat gaagtgactg gaaaaatgat aacgcgggat
     3721 aatccaccaa tcccgggaaa gcaatcgctg gcgaaggtga cgtcaattcc tctggtcagt
     3781 ccggttgagt tttcgacatc atcgtggcgg cgggctgttc tgtctcttga agaacatcat
     3841 aaagcctggt tgttgtggtg ttacagcggg agtatttgtt gggaatatca gatcgcgata
     3901 acacagtggg cgtggaatga atttaatact caatccggta ccagaaaaat tgctgggaaa
     3961 acgcaggaac gcctgaaaaa attaatctgg ctggcggcgc aggcagtaaa agcagaactt
     4021 tttggtgggg aaggttatga atatcaggag ctggcattac tggcgggagt gacaactaaa
     4081 aactggtcca aaacatttac tcgtcactgg gttgcaatga aacacatttt tcaccgactg
     4141 gatagtgagg ctttattatt tgtaatgaga acacgttcaa aacaaaaggc ggcattttca
     4201 aagcaaagtg ttgcaaaagt agattgaaag gcatatattt catgcaaatc tgatattttg
     4261 ccgattttgt acgtgatggc aaaagcaaac aaaacccgcc cacaagcggg ttttgttgtg
     4321 ccacttatct cggatagaca tggtgaatgc gctggtggag gagataaggg tgatttttgt
     4381 atgcttgcaa cattgatttc gtaacgttat tatcctgcgc ccggcccttt agctcagtgg
     4441 tgagagcgag cgactcataa tcgccaggtc gctggttcaa atccagcaag ggccaccaac
     4501 cgccactagc catcaggaaa gagcgtcaac cctttaagtt gagtgtgcga ggttcgagtc
     4561 cccggtggtg gtccattatc ggtattctgc gttgttagct cagcaggaca gagcaattgc
     4621 cttctaagca atcggtcact ggttcgactc cagtacaacg cgctacactt attttcccgg
     4681 ctcgcttttg cgggcctttt ttgtatctgc gccacgcccg gcgcatacca accacagagc
     4741 ctttc
//
LOCUS       DAZUGK010000272         4740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZUGK010000272 DAZUGK010000000
VERSION     DAZUGK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.274"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(462..2000)
                     /gene="aldB"
                     /locus_tag="ACHY5W_004389"
     CDS             complement(462..2000)
                     /gene="aldB"
                     /locus_tag="ACHY5W_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFS4449146.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2108..3403)
                     /locus_tag="ACHY5W_004390"
     CDS             complement(2108..3403)
                     /locus_tag="ACHY5W_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS4449147.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVEVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRAPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLIRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQADVIKRA
                     LHNLQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVSETSAS
                     LGVSDNTARTDLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3533..4684)
                     /gene="yiaY"
                     /locus_tag="ACHY5W_004391"
     CDS             complement(3533..4684)
                     /gene="yiaY"
                     /locus_tag="ACHY5W_004391"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFS4449148.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1133 a         1248 c         1154 g         1205 t
ORIGIN      
        1 cgacatattg ttttttatat actttctgaa gtgtgctgcc tggcttttta acaggtcgag
       61 acttatcata ttgaaataat ggaactattg gccaaattta tgattaaaaa ccgtaaaaca
      121 tccacaatgc agattaagcg tattaaacct tcaataatct accgtttatt gctgattgtc
      181 ctcggaatac caatggtgat ttacggtctg gtttgcccgc tcaccatcga aacgcgagat
      241 taaactcctg gcaaaacacg ccccagaaac gcacttttgt tggcgtagtc tgggttattg
      301 cacacgtagg tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc
      361 gtgtacgcct tatccggcct acggtcaggt ccccgtaggc atgataagac gcgtaagcgt
      421 cgcatcaggc aattaatacc cgatgcgacc agcttcttac atcagaacag ccccaacggt
      481 ttatctgagt agctcactag caggcacttg gtttgctggt aatgctccag catcatcttg
      541 tgggtttcgc gaccgatacc tgattgtttg tagccaccaa acgccgcatg tgccgggtaa
      601 gcgtgataac agttggtcca cacgcgcccg gcctgaatgc cgcgccccat cttataggcc
      661 agattaccgt tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc
      721 tccagcgctt cttccatcgt tttgaaggtg gttaccgcca gcaccgggcc aaaaatctcc
      781 tcctggaaca cgcgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg
      841 tctttcagtt cgccttccag cagcttacgc cgcccgccgg tgagcacgtc tgcgccctct
      901 tttttaccga tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgca
      961 cccatttgcg tcacgctgtc gagcgggtta ccgctgcgaa tgctttcgac acggcggatg
     1021 gcgcgttcca taaagcgttc gtagattgac tcctgcacta aggcacgact cggacaggtg
     1081 caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag
     1141 gcatcttctt catccatcac atcagcaaag aagatatttg gcgacttacc gcccagctcc
     1201 agcgtcaccg gaataatgtt ttgcgttgcg tattgcataa tttgttggcc cacttccgtt
     1261 gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc
     1321 ccacctgcgc cattgaccac attcaccacg cccggtggca gtaaatcacc gacaatttcc
     1381 attagcagca gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac acagttgccc
     1441 gccgccagcg cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata
     1501 atctgcccca ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatcaact
     1561 tcactgatcc caccttcctg cgcccgaata cacgaggcga aatagcggaa atggtcaatc
     1621 gccagcggta catccgcagc actggtttcg cgaatgggtt tgccgttatc ccaagtttca
     1681 gctgtcgcta acagctcgag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc
     1741 gcgcgatcct gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc agcatccagc
     1801 gccagatcga tgtctcgttt gcccgaagac gccacttcgc acagcagctg cccggtcacc
     1861 ggcgtcaggt tctggtaata ctcaccatcg gcaggggcta cccattcgcc gccaataaag
     1921 ttgtcatagc ggttttttaa cttgaggggg aaaccatact cgccgggctt aatctgtgct
     1981 gaagggggat tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg
     2041 tggctggcga aatcttcgcc agtcggtaac agctttacga cggctatcac gaatttacgg
     2101 gcaagggtta cagcggataa tgcgcgaagt acactgcttg ctggtcatta attttcttct
     2161 cttgcgcaaa tccttcttta acaatcgtcc gtaaatcagt tcgcgctgta ttatcagaga
     2221 cgcccagact cgcgctggtt tcagaaactg tgtactcttt ccccggattg gcgagcatca
     2281 cattcaggag cgttacctgg cgaggattca ggcgtttaag cacaccagag ctaaacagca
     2341 cactatcgag ttttgcgctt cgttgtgtaa tttcatcaat atgctgctgc aagttatgca
     2401 gagcgcgttt aatgacatcg gcctgatatt caaggaaata agtcagatcc ataccgtctg
     2461 attcagtgta ctggtagctg gcagcatatt tgactggcgc agcatgaagc aaacggctga
     2521 tggaaatata tttaaagaca tcgtagcccg atttcagcat ataccagtaa aacagcgccc
     2581 gactggtgcg cccgttgcca tcgcgaaatg gatgttcatg ggccagcata aaatgcagaa
     2641 tgcaggcacg gattaacggg tgaatatacc cttcatggct gtttaaccac tggcaaactt
     2701 tttccagtcg ttccggtaac aatgccgcag cgggcggttg gtgcacaata ttgccgtcat
     2761 aatcggcgat cacgacatca tctgtttcac gaaattcacc tggacgatat ttcgcgtcat
     2821 ttattccccc catgccaatg gcatgtaact gacgtataag cgcaggtgtc agtggttctg
     2881 cgagtaagtg cggaatttcc gccatcagcc gggcatttcc cgcaatcata tgctcgtctt
     2941 cagtgcgtgg cgctcgccct gtttccagca tttttctcgc caccaacgtc gtggtattcg
     3001 caccttccag ttgggaactg gtgatcggct cttccaggcg taactgactt aactcggcgc
     3061 ccgccccgcg taaccgttca gtctgatctt tcagcgccag tgcagtggca tgtttatcaa
     3121 caagctcgca cgcttctgca atcactggtg taatgttgaa tccggcctgc tcacccgctt
     3181 cattgcaata attgattcgc tggatcgccg cgttgcgcgc cagccgggta agcgtccacg
     3241 ccacttcgac gttttctcct tttttgatac ggtactgcaa ctcatcaaaa ggcagataac
     3301 gtcctttttc atcggtaacc cggttaaagc gcaggtgggc caggagttcc tcacccgata
     3361 agtgtgataa aggaataagt ccgggaggtg atttgatagc catgtgctta tctcaaaaaa
     3421 gcgcaatatt gagatagtgt atagcaggaa agagaaacta tctcaaaaaa agcaagtttt
     3481 gagatagttg aaaaagcggc tgacaatttg ccagccgttg ttggtatgat gattacattg
     3541 cggcgcgata aatcgccaca atttcttcgt gagttgcctg gatcgggtta gtaaagccac
     3601 aggcatcttt cagggcatta gtcgccagaa ccgcgaaatc ttcttctttc acgttcaggt
     3661 cgcgtaggcc tgccgggata tccactttct tcgccagttc acggatggcg ttaatgcagg
     3721 cttcagcacc ttccgcgtcg tttttacctg tcacgttcac gcccattgca gcggcacagt
     3781 cacgcagacg tgcagcggcg actttgctgt tgaatacctg aacatgcggc agcagaacgg
     3841 cgttacatac accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa
     3901 cataacccag agaagcatta ttgaacgcca taccggcaag gaactgggca taagccattg
     3961 cttcacgcgc tttcgcatta ctgccatctt caacggctaa cggcaggttt tcggcaatca
     4021 tggtcacggc tttcagtgca caagcgtcag tgatcggcgt ggcggcaata gaaacatacg
     4081 cttcgatagc gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac ttcggcatac
     4141 caatcatcag agaggagtca ttgacagaaa gcagcggagt gacatgttta tcaacaatcg
     4201 ccattttgat atgacgcgct tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg
     4261 tacccgccgt ggtattgatg gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc
     4321 cttcgtaatc gcgaatatcg ccgccattgg ctgccaccag cgcaatacct tttgcgcagt
     4381 cgtgcggaga accaccgcct aaggagatca cgctatcgca attattttct ttaagtaatt
     4441 tcaaacctgc ggcgacgttt tccgtggtgg ggttaggttg ggtgccatca taaataatgc
     4501 taaaaatatt gcgttcttcc agtgcttttt gcacatcgcc cgccatacct aatttcgtta
     4561 acatactgtc agtgacaatc aatgtacggc gaaacccata ttctgccatc atattcattg
     4621 catcagccaa tgaatcagcg ccgatgacat tcacagaagg aataaaaaag gttgaagctg
     4681 ccataacaca ctcctgaaag tgtaaaaaag ggcgggcagc ttactgagga ttttcattcg
//
LOCUS       DAZUGK010000273         4546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZUGK010000273 DAZUGK010000000
VERSION     DAZUGK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.275"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..1232
                     /gene="proB"
                     /locus_tag="ACHY5W_004392"
     CDS             129..1232
                     /gene="proB"
                     /locus_tag="ACHY5W_004392"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4449149.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATTEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1244..2497
                     /gene="proA"
                     /locus_tag="ACHY5W_004393"
     CDS             1244..2497
                     /gene="proA"
                     /locus_tag="ACHY5W_004393"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4449150.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAKDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNLADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2612..2687
                     /locus_tag="ACHY5W_004394"
     tRNA            2612..2687
                     /locus_tag="ACHY5W_004394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Thr,seq:cgt)
     gene            complement(2815..3714)
                     /locus_tag="ACHY5W_004395"
     CDS             complement(2815..3714)
                     /locus_tag="ACHY5W_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449151.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            3813..4535
                     /locus_tag="ACHY5W_004396"
     CDS             3813..4535
                     /locus_tag="ACHY5W_004396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4449152.1"
                     /translation="MDKVILITGASSGIGEGIARELGTTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WELTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT         1133 a         1131 c         1229 g         1053 t
ORIGIN      
        1 gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca gatggttggc
       61 aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa attgaatggc
      121 agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt gtgctaacag
      181 gcggatcgcg ccgcctgaac cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt
      241 tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc gccggacgtg
      301 agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg
      361 tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat ggcattcacg
      421 tcgggcaaat gctgctgact cgtgctgata tggaagatcg tgaacgcttc ctgaacgccc
      481 gcgataccct gcgtgcgctg ctcgataaca atatcgttcc ggtaattaat gagaacgatg
      541 ctgtcgctac gacagagatt aaagtcggcg ataacgacaa cctttctgca ctggcggcga
      601 ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg tataccgctg
      661 acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt gatgacgcac
      721 tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg agtaccaaat
      781 tgcaggccgc ggacgtggct tgccgtgcgg gtatcgacac cattattgcc gcgggcagca
      841 agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg ttccatgccc
      901 aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgcct gcgggtgaaa
      961 tcacggtaga tgaaggggca accgccgcca ttcttgaacg cggcagctcc ctgttgccga
     1021 aaggcatcaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc atttgcaacc
     1081 tcgaaggtcg cgatatcgcc cacggcgtca gtcgttacaa cagcgatgca ttacgccgta
     1141 ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatac ggcccggttg
     1201 ccgttcaccg tgatgacatg attacccgtt aaggagcagg ctgatgctgg aacaaatggg
     1261 cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg
     1321 cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc tcaacgctaa
     1381 cgccaaggat gttgctgacg cgcgtgccaa tggcctcagc gaagcaatgc ttgaccgtct
     1441 ggcactgacg cccgcacggc tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct
     1501 cgccgatccg gtggggcagg taatcgatgg cggcgtactg gacagcggcc tgcgtcttga
     1561 gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgtc cgaacgtgac
     1621 ggttgatgtc gcttcgctgt gcctgaaaac cggtaacgcg gtgatcctgc gtggaggcaa
     1681 agaaacctgt cgcactaacg cggcaacggt ggcggtgatt caggacgccc tgaaatcctg
     1741 tggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga
     1801 aatgctgcgt atggataaat acatcgacat gctgatcccg cgcggcgggg ctggtttgca
     1861 taaactgtgc cgcgaacagt cgacgatccc ggtgatcaca ggtggtatag gcgtatgcca
     1921 tatttacgtt gatgaaagtg cagagatcgc tgaagcatta aaagtgatcg tcaatgcgaa
     1981 aactcagcgt ccgagcacat gtaatacggt agaaacgttg ctggtgaata aaaacctcgc
     2041 cgatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga cattacacgc
     2101 agatgcagct gcgctggcgc agttgcagac aggccccgcg aaggtggtgg ctgttaaagc
     2161 cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct
     2221 tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg cgatcctgac
     2281 ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg ctgtttacgt
     2341 taacgcctct acgcgtttta ccgacggcgg ccagtttgga ctgggtgcag aagtggcggt
     2401 aagtacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga ccacttacaa
     2461 gtggatcggc attggtgatt acaccattcg tgcgtagata aaaccgggtg atgcaaaagt
     2521 agccatttga ttcacaaggc cattgacgca tcgcccggtt agttttaccc ttgtccaccg
     2581 tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg tagagcagcg
     2641 cattcgtaat gcgaaggtcg taggttcgac tcctattatc ggcaccattt aaatcaagta
     2701 gttactccat atttaaatac accacctttt cctgctgtgc cgtatttgtg ccattgtaac
     2761 cctggcaatt aatcaaaata ctgttctgac atcaggcaat acaggtgcag acatttaagc
     2821 caattgctgc cgccattcct tgacgtggtc aatcagggcg cggagctttg gtgcaatatt
     2881 gcgacgctgt gggaaataca gatagaagcc cggaaattgt ggaagaaagt catcaagcag
     2941 agatacaagc ttaccgcttt caatatatgg cctgaaagtt tcctgagtgg caattgttat
     3001 tcctccgccg gcaagagcca gcctcaacat cagacgcaga tcattagtcg taatctgtgg
     3061 ttcaatcgca aggtcgaaag ctctcccgtt ttcttcaaat ggccagcgat aaggcgcaac
     3121 atccggggat tgacgccagc cgatacactt atgattcaca agttcacgcg gatgaacagg
     3181 tgtgctgttg gctgcgagat aggagggaga ggcaacgacc atttcacgct gcctgccggt
     3241 aaggggcaca gcaatcatgt ctttttcaat gacctctccg agccttacgc cagcatcgta
     3301 acctgcggcc acgatatcga attcctcatc cgttacaaca atatcaagcg taacggctgg
     3361 attggcagct gcaaacgaag cgattagcgg acctgaaaga aattcttctg ctatcgacgt
     3421 caccgcgatt ctgagaagtc cgcgtggcac atggtcagaa accatctctt cgaacgctgc
     3481 ctcaataccg gataaaggta atgacagcga tttatgcagc cgttctcccg cttctgtcaa
     3541 attaactgag cgcgtggtcc gcattacaag cattgtcccg aatgcatctt caaggcgtct
     3601 tattccctga ctgaccgctg aacgggtaac gcccagccgt gcggcggctt tactaaaatt
     3661 actctcttct gctaccgcga taaaaatggg taaaagatta agatcgattt tcattgttta
     3721 atgctgctaa ccactgagta cattgaaagc aggatactgg aaccaatcat aaagttctat
     3781 tctctctccg aacacatttg aaggagaacg taatggataa ggtcatatta attaccggcg
     3841 catcaagtgg tattggggaa ggtattgcca gagagctggg aacgacaggt gcaaaggttt
     3901 tactgggagc acgcagagtt gaacgtatcg aagccattgc aaccgaaatc tgccgcgcag
     3961 gaggaattgc taaagcgcgg gagttggatg tcacagaccg acagtccatg gccgatttcg
     4021 tgcaagcagc gctggatagc tgggggcgag ttgatgttct tatcaataat gcgggcgtta
     4081 tgccgctttc accgcttgca gcaggaaaac aagatgaatg ggagctcact attgacgtga
     4141 atatcaaagg tgtactgtgg ggaattggcg ctgtacttcc ggtgatggaa gcacagggtt
     4201 ccggtcagat aatcaatctt ggttctattg gtgccctttc tgttgtgccc acaggcgcag
     4261 tctattgtgc atcaaaattt gcagtacggg ctatttctga tggcttacgg caggaaagct
     4321 cgaaaatccg tgtgacctgc gtaaatcctg gcgttgtcga aagtgaactg gcttcaacca
     4381 ttacccatgt ggaaaccatg aaggcaatgg acgcctaccg ttctgttgca cttcaacctg
     4441 cggatattgc ccgtgcggta aggcatatta ttgaatctcc cgaaagcgtt gatacaaccg
     4501 aaattaccat cagacccact gcatctgcaa actgaagaat aacttt
//
LOCUS       DAZUGK010000274         4521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZUGK010000274 DAZUGK010000000
VERSION     DAZUGK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.276"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..194)
                     /locus_tag="ACHY5W_004397"
                     /pseudo
     CDS             complement(1..194)
                     /locus_tag="ACHY5W_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            344..1012
                     /locus_tag="ACHY5W_004398"
     CDS             344..1012
                     /locus_tag="ACHY5W_004398"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4449153.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(957..1094)
                     /locus_tag="ACHY5W_004399"
     CDS             complement(957..1094)
                     /locus_tag="ACHY5W_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFS4449154.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1067..1651)
                     /locus_tag="ACHY5W_004400"
     CDS             complement(1067..1651)
                     /locus_tag="ACHY5W_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS4449155.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1651..>4521)
                     /locus_tag="ACHY5W_004401"
     CDS             complement(1651..>4521)
                     /locus_tag="ACHY5W_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449156.1"
                     /translation="TTVVVNTLASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGT
                     ITVYEDSRPGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSAG
                     DASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASKSAAAA
                     ESSKSVAATSAGAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKS
                     SETNASSSASSAASSATAAGNSAKAAKTSETNAKSSETAAGQSASAAAGSKTAAASSA
                     SAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAKTSETN
                     AKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTKATEAA
                     GSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSE
                     SLAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSTSEMLAATPKSVKAAYD
                     LANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSARKVNG
                     KALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVG
                     SPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYAT
                     GVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDT
                     VPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKS
                     HTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHSHVDGRRFNTS
                     TFKDTYQYGSTTVGSNSYQVQGAVGLGIGTMANTNSAGAHTHSLSGTAASAGAHAHTV
                     GIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          983 a         1198 c         1112 g         1228 t
ORIGIN      
        1 ttcgagatag taatgactgt tactgtaaca tgagttacaa acagcttcat acccagagct
       61 actgatgtat caactcgatt caacaatacc cgatatttat tccatgcctc cagcaacgat
      121 ctttcttcct ccgttgcgat ttccagatct acagcatcct gcagtggcgc aatatactca
      181 ctgaattcct ggatgtagaa ctgtgtggtg acggtcttcc agccattcgg ctcctgttgt
      241 atcgaagcat accaggctat ttcaatatcg ctatgctgcg gcagcattta accccttgta
      301 attcatcgcc ataattgatt taattcacaa ataaaactat aacatggtga aattaatgaa
      361 aaaaaacaca gatgatgggg ctaaaattta cacaccactt accctaaagc tttatgactg
      421 gtgggttttg ggagtatcaa atcggcttgc atggggatgt cctacaaagg aacaccttct
      481 tccacacttt ctggaacatt taggtaacaa ccatctggat attggtgttg gaactgggtt
      541 ttaccttact cacgtacctg agagtagtct gatatcttta atggatttga acgaagctag
      601 cctgaacgcg gcatctacaa gggctgggga atcaaaaatt aaacataaaa ttagccatga
      661 tgtttttgaa ccttatcccg cggcgttaca tggtcaattt gattccattt ccatgtttta
      721 ccttcttcac tgcctgcctg gaaatatatc tacaaaaagc tgtgtaatac gcaatgcggc
      781 gcaggcctta actgacgatg gaactctata cggagccaca attcttggcg atggagttgt
      841 gcacaatagc ttcggtcaaa aactgatgcg catttacaat cagaaaggca tcttttcaaa
      901 cacaaaagat tccgaagaag gcttaacaca tatactctca gagcatttcg agaatgttaa
      961 aaccaaggtt caaggtactg tagtaatgtt ttccgcttca gggaaaaaat agcatccaac
     1021 cgcagcacgt tcttgcttaa gacgtgctgc ggcataatcc caatgattac tccctgacag
     1081 ggttcgtagg ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc
     1141 gatacttttt ccaggcttcc agcaatgagg tttcttcctc cgttgcgatt tccagatcta
     1201 cagcatcctg aagtggcgca atatgctcac tggctacctg catcaggctg ttttttattt
     1261 cttccgcctc tcggatccgg aacagttttt ctgcttctgc atctttcacc caggctgtgc
     1321 cgttccactt ctgaaactcc ccttccggcg ataaccaggt aacattttcc ggtaacggac
     1381 cgagttcaga aataaataac tcgtcccctg acgctacgtc ataaaccgtt ttaccccgat
     1441 ggtcttcaac gagatgccac gatgcctcat cactgttgaa aacagccaca aagccagcag
     1501 gaatatctgg tggtgcaata tcggtactgt ttgctggcag acctgtatga ggcggaatat
     1561 atgcatcacc ttcaccaata aattcattag ttccggccag cagattataa atttttatgg
     1621 tccgtgcttg ttcactcatt ctgaatgcca ttatgcaagc ctcacaatat agttaaatgc
     1681 gatgtttttg acggtgtttt ccgcgttacc agcagcgtta acggtgatgg tgtgtccatg
     1741 tgagccaatc gcaacggagt gcgtatgagc accaataccg acagtatgtg cgtgtgcacc
     1801 tgcgcttgca gcagtgccgg acagtgagtg ggtatgcgcg ccagcagagt tcgtattcgc
     1861 catcgtgcca atgcccagac caaccgcccc ttgtacctgg tatgagttac ttccgacagt
     1921 tgtagaacca tactgatagg tgtctttaaa agtgcttgta ttaaacctgc gaccatcaac
     1981 gtgcgagtgt gctccggccg agcttgtaga gccgctcaga ctgtgcgtat gagccccggt
     2041 gttattcgtg gatttagtgc cgtaatcaaa cgacgatgtg gttttcgtcc ccaaatccgt
     2101 actggatgcg ctggcgctgt gggtgtgcga tttaatgccg tcctgttcct gagacaatac
     2161 ggcacgacca ctggcgggtt tgcccttaat cgtccagcca cgcatatcag ggatcacgcc
     2221 tgacggataa gcggctgcaa gtttcgggta tgcagatttg tcaaaagtct gcccctgcat
     2281 cagggcatag ccagacggaa cggtatctga tggccacggg attggtgcgc caggcggata
     2341 aaactgctct gatggcgtat agagtgaata aactgtaccg tccgttaacc cttccggctt
     2401 attagcagaa tatgctggtg acgtatgaat cgtcacgctg gcattactgg tataatccca
     2461 ttgaatattt acaccagtcg cataatttcc gattgcaacg taaatatcgt aagtatcacc
     2521 agatgtattg acccaggcaa aatttgtaaa ccctgtcgat gtgcgctgcc ataaagcacc
     2581 agtaatcccc ttcggattac cattacctgc acgcaaaaca agttcagata tacctgcctg
     2641 ttgaggtgac cccacgttaa atcccgcgcc accaatcaac gtaattgaaa caacagaact
     2701 cgcctgtggc atggttaccg ttgctaattt gaaccaacca gcaccaccgc tgaatgacat
     2761 tgttgttgag ttaagcgtac caatatcttt cggcgtcagt gttatatccg ctgaaagcgc
     2821 cttaccattc accttacggg cagaaggtac ccgaccattc gcattatcat tagctgcttt
     2881 cactgctttc ggtgtcgcgg caagcgtttc agatgcgctg ttggttgcac tactgagctg
     2941 gacaattcct ttttgtgctg tcgtagcgtc ctgagcggta tatttcccgt tagcaaggtc
     3001 ataggctgcc tttaccgact ttggcgttgc cgccagcatt tcagatgtgc tgttggtcgc
     3061 gctgctgagc tgaacgatcc ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc
     3121 gttagccagg tcatacgcgg ccttaactgc ttttggcgtt gccgccagtg actcggaagt
     3181 gctgttagtc gcactgctga gctgtactat cccctttttc gtcgtgctcg catcctcaag
     3241 cgccacggcg gatgcaatat cctctgcccg tttagctgct gtctcggcgc gcgttgccgc
     3301 ggattccgcc gtacttttgc tctgagctgc cgccgtcgca ctgccagctg cctctgtcgc
     3361 cttcgtggat gccgtcgtgg cgctgctctt cgctgctgac gcctgtctgg tcgcctcatc
     3421 ttttgaagca gacgccgatg atgccgatga cgccgccgaa ctggctgacg atgcggcagc
     3481 cgtttttgag gattctgcgc tggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc
     3541 tgcggaagca gaactcgctg ctgcgctggc ctgttcagtg gcttcgccag ccttcgttgt
     3601 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc
     3661 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     3721 gcctgccgca gctgaggcgc tctgtcccgc tgccgtctca gaagacttag cgttcgtctc
     3781 ggacgttttt gccgccttcg cggaatttcc tgccgccgtt gccgaggaag cggcactact
     3841 ggcgcttgat gatgcgttcg tttctgatga ttttgccgct tcttttgaag ccgacgcatc
     3901 ccgggctgag gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggctgc
     3961 tgattgttgt gacgctgccg cattcgtttc tgacgttttc gccgcaccgg cactggtagc
     4021 tgccacgctt tttgaggact ctgcagcggc agcacttttt gaggcttcag tggcctttgt
     4081 tgatgccgtt cctgcgctgg aagacgctga ctgagccgac gaagcggcct gtccggctga
     4141 cgtgctggct gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc
     4201 tgatgtgctg gcatcgccgg ctgacttctt cgcggctgcc gtgttctgtg ccaccgcgga
     4261 cgcgttacgc gccacctctt ccaccatcag ttcaaaacgg cgcagtgcct caggacgggc
     4321 atcatcctcc gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata
     4381 cacggtgatg gtcccggcat gtgacggcgg gaatccctcc accaacagaa taacgctgta
     4441 ctgcccgtac tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag
     4501 cgtgttcacc accaccgtgg t
//
LOCUS       DAZUGK010000275         4516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZUGK010000275 DAZUGK010000000
VERSION     DAZUGK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.277"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..455
                     /locus_tag="ACHY5W_004402"
     CDS             <1..455
                     /locus_tag="ACHY5W_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449157.1"
                     /translation="FGPPKTNAGIRTVTLLRPAIEALKEQYKLTGHHRKSEITFYHRE
                     YGRTEKQKLHFVFMPRVCNGKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFAC
                     WLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQVAMLNARLS"
     gene            508..1443
                     /gene="ttcA"
                     /locus_tag="ACHY5W_004403"
     CDS             508..1443
                     /gene="ttcA"
                     /locus_tag="ACHY5W_004403"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFS4449158.1"
                     /translation="MQENQKNTKKEIYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1572..2945)
                     /gene="dbpA"
                     /locus_tag="ACHY5W_004404"
     CDS             complement(1572..2945)
                     /gene="dbpA"
                     /locus_tag="ACHY5W_004404"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFS4449159.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEALRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(2975..3148)
                     /gene="ynaL"
                     /locus_tag="ACHY5W_004405"
     CDS             complement(2975..3148)
                     /gene="ynaL"
                     /locus_tag="ACHY5W_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFS4449160.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3423..4406)
                     /gene="zntB"
                     /locus_tag="ACHY5W_004406"
     CDS             complement(3423..4406)
                     /gene="zntB"
                     /locus_tag="ACHY5W_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFS4449161.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4420..4467)
                     /gene="ydaN"
                     /locus_tag="ACHY5W_004407"
     CDS             complement(4420..4467)
                     /gene="ydaN"
                     /locus_tag="ACHY5W_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFS4449162.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1198 a         1165 c         1100 g         1053 t
ORIGIN      
        1 tgttcggtcc cccaaaaaca aatgcgggga ttcggacggt aacactactg cggcctgcta
       61 ttgaagcact gaaggagcaa tacaaactaa ccgggcatca tcgcaaaagc gaaatcactt
      121 tttatcatcg ggagtacggc agaaccgaaa agcaaaaact gcattttgtt ttcatgccca
      181 gggtgtgtaa cggaaaacag aaaccttatt actcggtaag cagtctgggg gcgagatgga
      241 atgcagcagt aaaacgtgct ggtattcgcc gccgtaatcc gtaccatacg cggcatactt
      301 ttgcctgctg gttgttgacg gcaggagcga acccggcatt tatagccagc cagatggggc
      361 atgaaactgc gcagatggtg tatgaaattt acggtatgtg gattgatgac atgaacgacg
      421 aacaggtagc tatgttgaat gcgcggttat catagttgca aagtttgccc cccaatttgc
      481 cccatttagt accagagaac tgaaataatg caagaaaatc aaaagaatac gaagaaagag
      541 atatacaatc tgaacaaatt acaaaaacgc ctgcgtcgta acgtgggcga agccattgct
      601 gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctccgg gggtaaagac
      661 agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat caatttttcg
      721 ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cggaacacgt tctgcccgag
      781 tatcttgaaa agctgggcgt tgagtacaag attgttgaag agaacactta cggtatcgtg
      841 aaagagaaga ttccagaggg caaaaccact tgctcactgt gttctcggct acgtcgcggt
      901 atcctttatc gcaccgcaac ggaactgggg gcgacgaaga tcgcgttggg ccaccatcgt
      961 gacgatatcc tgcaaacgtt gttcttaaat atgttctacg gcggcaagat gaaaggtatg
     1021 cctccgaaac tgatgagcga tgatggcaaa catatcgtta ttcgtccgct agcctactgc
     1081 cgcgagaaag atattcagcg atttgccgat gcaaaagcgt tcccgattat tccgtgcaac
     1141 ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt gcgtgactgg
     1201 gataaacgtt atccagggcg tatcgagacg atgttcagcg cgatgcagaa tgtggtgccg
     1261 tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca tcacccacgg ttctgaagtg
     1321 gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc ggcgggctgg
     1381 cagccagaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt tgaagtgaaa
     1441 taaccaggat agcgcccgat gcgcaagcgt atcgggctac tcttatggag gccggataag
     1501 acgcggccag cgtcgcatcc ggcaatcccg aataagatgt ttactcttgc acccggcaat
     1561 tcaacatttt attattttaa taaccgcacc cggcacgttt ttcctttaat cttcccgccc
     1621 tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata gacatgcgcc
     1681 ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc tgtcagtgca
     1741 cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca caacgttgcc
     1801 atttctgctt ccagcgtcac aatggaacta ttagctggcg gcgtttgcca gttaagtttt
     1861 atctgcaaca tgtcagaaat gatattggcc cgcagtgctt cttccggagc acagaaactg
     1921 atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac atgaacttca
     1981 gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc cagaccacgc
     2041 gcagcaacat cagtcgcgac cagtacacgg gcgctaccgt tagcaaaacg taccagggtc
     2101 tgatcgcgat cgcgttgctc caaatcgccg tgtaatgaca atgcactttg ccctacttca
     2161 ttcagcgcgt cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacgcaagag
     2221 gatggctgat gcaagcttaa taaccgttgc aacagaggaa ttttgccttt gctggatgtc
     2281 tcataaaatt gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa
     2341 ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt ccggccaggt tgccgaaaac
     2401 agaagcgtct gtcgagatgc tggcgcaaaa cggatgacat catcaatggc atcgctaaat
     2461 cccatatcca gcatgcggtc ggcctcatcc atcaccagcg tattcaacgc atccagtgat
     2521 accgtgcctt tttgcaggtg atccagcaaa cgccccggcg ttgccacgat aatatgcggc
     2581 gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt
     2641 ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt
     2701 tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt
     2761 aacaagccga ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca
     2821 tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat agttaaataa
     2881 cccaactcat taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg
     2941 gtcacaatct attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc
     3001 tacgctcacg atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt
     3061 gcgggcgagg gatcggatcg ggcactggaa caggatcgcc aggaatcggt tcagggacag
     3121 gaatttgcaa ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa
     3181 gggtagacgc tgataaataa caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac
     3241 gaatcaattg tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc
     3301 ccatcgcttg acgttgcatt cacctgcaag agagatattg ccctgaatgg gtagagagtt
     3361 tattgacttc gctcaaactt tgcggcgttt ttgtatacag acagccggaa aaattgcttt
     3421 tgttacaacc atttactacg atgcaaccat aaagcaacac caccaataag aacaactaac
     3481 agaatacaaa aaattgaaaa tccgaattgc cacccgccgc cagggatccc accaaggttg
     3541 acgccaaata accctgtcag aaaggtactg ggtaaaaaga ccattgccat caatgacatt
     3601 gtataggtac gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcaca
     3661 ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata
     3721 tcctgcatcc gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga
     3781 gcatagacat cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc
     3841 agagccagga atccacgcgg cggaatttgc tgatcaagga gattatcttc aaggtcgata
     3901 attttatcgt gcagctgctc gataaattcg ctggaatgat cggtcaacgc atcgcacaca
     3961 tccaccagcc accctccgca atcggtcgga cccgtgccct cttccagatc gctcaccaca
     4021 tcgtccagcg ccaacacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat
     4081 acacgcatgg cgaccagttg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat
     4141 gtaatcagcg tgccttcacc gagacggctg actcggggcc gtgtgctctc acctgccagc
     4201 gcatcacgta cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg
     4261 tgtacataat taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc
     4321 agcggtttaa cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta
     4381 acgtccgatc ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc
     4441 tagccttctg gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa
     4501 aattctgtct aaattg
//
LOCUS       DAZUGK010000276         4506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZUGK010000276 DAZUGK010000000
VERSION     DAZUGK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.278"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..3859
                     /gene="espP"
                     /locus_tag="ACHY5W_004408"
     CDS             <1..3859
                     /gene="espP"
                     /locus_tag="ACHY5W_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFS4449163.1"
                     /translation="LIAVSELSGRVSSRATGKKKHKRILALCFLGLLQSSYSFASQMD
                     ISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTTSI
                     GGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGATEG
                     ADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLNGS
                     FVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNYAN
                     GKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTGTGSDTTDIKNKDLIF
                     TGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSTVEWNIRYDNK
                     DNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENALSG
                     GEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSILSLNNTADYIYHG
                     NINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGAFS
                     CSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFGTL
                     HLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVRGN
                     SQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIENGADVTTQSGMFSTSD
                     ISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKSAS
                     IMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGDSA
                     LKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNLSESDKLEVKKHLSGE
                     NNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGFSDVTPVITTRETDDK
                     ITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWARI
                     MSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSV
                     GAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHV
                     TEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLE
                     FEKSAFGKYNVDNAVNANFRYSF"
     gene            4403..>4506
                     /locus_tag="ACHY5W_004409"
     CDS             4403..>4506
                     /locus_tag="ACHY5W_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449164.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
BASE COUNT         1363 a         1012 c         1028 g         1103 t
ORIGIN      
        1 gttaatcgct gtttctgaat tatccggcag agtatcatca agagcaactg gtaagaaaaa
       61 acacaaacgc atacttgcat tatgtttttt aggcttatta caatcctcat attcttttgc
      121 gtcacagatg gatatttcaa atttctacat ccgtgactat atggattttg cacagaacaa
      181 gggcatattt caggctggcg caacaaatat tgaaatagtg aagaaagatg gctccaccct
      241 gaaactaccg gaagtaccat ttcctgactt ctcaccggtt gcaaacaaag ggtcaaccac
      301 atctattggt ggtgcataca gtatcacagc cacacacaat acgaaaaacc accactcagt
      361 tgcgacgcaa aactggggga acagcacgta caaacaaact gactggaata cttcacatcc
      421 tgattttgca gtatcccgac ttgacaagtt tgttgttgag acccgaggtg cgactgaagg
      481 cgcagatatt tcgttatcaa aacagcaggc acttgaacgt tacggggtta attataaagg
      541 agaaaagaaa cttatcgcat tcagagccgg ctctggtgtg gtatccgtta aaaaaaatgg
      601 acgcataact ccatttaatg aggtttctta taagccagaa atgttaaatg gctctttcgt
      661 tcacattgat gactggagtg gatggctgat attaaccaac aaccagtttg atgagtttaa
      721 taacattgcc tctcagggtg acagcggttc agcactgttc gtctatgata accaaaagaa
      781 aaagtgggtt gtcgctggaa ctgtctgggg gatttataat tacgccaatg gcaaaaacca
      841 cgcagcatac agtaaatgga accagacaac cattgacaac ctgaagaaca agtattctta
      901 caacgtggat atgtcaggag ctcaggttgc aaccattgaa aatggaaaac tgacaggcac
      961 tggctcagac accaccgata taaaaaataa ggacttaata tttactggcg gtggagatat
     1021 cctcctgaaa tcctcttttg ataatggtgc tggcggtctt gtctttaatg ataaaaagac
     1081 ctatcgagta aacggggatg atttcacctt taaaggtgcc ggtgttgata caagaaacgg
     1141 cagcaccgtt gagtggaata tccggtatga taataaagac aaccttcaca aaattggtga
     1201 tggcacatta gatgtccgaa aaacccagaa caccaacctg aaaacaggtg agggtcttgt
     1261 cattcttgga gctgaaaaaa cattcaataa tatctacata accagtggtg atggaactgt
     1321 ccgactgaat gcagaaaatg cactgtctgg cggtgaatac aacggtattt tctttgcgaa
     1381 aaatggcgga actcttgacc tgaacggata taatcagtct ttcaataaaa ttgctgcaac
     1441 tgattcaggt gctgtaataa ccaatacgtc aaccaaaaaa tccattttat ccctgaataa
     1501 tactgctgac tatatctatc acggcaacat aaacgggaat ctggacgtac ttcagcatca
     1561 tgagacgaaa aaagagaacc gtcgtcttat tcttgatggg ggcgtggaca caacaaatga
     1621 tataagcctg cgtaatacac aactgtccat gcagggacat gccactgaac atgccattta
     1681 tcgggatgga gctttctctt gttcactacc agctcctatg cgctttttgt gtggcagtga
     1741 ttatgttgca ggaatgcaaa atacagaagc tgatgctgta aaacaaaacg gaaatgccta
     1801 taaaaccaac aatgctgtct ctgatttatc gcagccagac tgggaaaccg gaacattcag
     1861 atttggaacg ctacatcttg aaaattccga tttttctgtt ggtcgtaatg caaatgtaat
     1921 cggggacatt caggccagta aatcaaacat tactattggt gacactacag catatattga
     1981 tttgcatgct ggtaaaaata ttaccggtga tggttttggc ttccgccaga atattgtgcg
     2041 tggaaactca caaggagaaa cgctgtttac aggagggatc acagcagaag acagcactat
     2101 cgttattaaa gataaagcaa aagcattatt ttcaaattat gtatacctgc tgaacacaaa
     2161 agcaaccata gagaacggtg ctgatgtgac aactcaaagt ggtatgttct ccacgagcga
     2221 tatcagcatc tctggtaatc tgtccatgac aggcaatccc gacaaagaca ataaattcga
     2281 gccctcaata tatctgaatg atgcttctta tctactgact gacgactccg ccagactcgt
     2341 tgccaaaaat aaagcatctg tggtgggaga tatacactcc actaaaagtg catccatcat
     2401 gtttggtcat gatgaaagcg acctctcgca gttgtctgac agaacctcaa aagggcttgc
     2461 acttggtctt ttaggtggct ttgatgtctc atatcgcggt tcagtcaatg ccccgtcagc
     2521 atctgccact atgaacaata cctggtggca actaaccgga gattctgcgc tgaaaacact
     2581 gaaaagtaca aacagcatgg tctatttcac tgacagcgca aacaataaga aattccatac
     2641 gctgacggtc gatgagctgg caaccagcaa cagcgcctat gcgatgcgta caaacctttc
     2701 tgaatcagac aaactggagg tcaaaaaaca cctgtctggt gagaacaata ttttactcgt
     2761 tgatttcctt cagaaaccaa cgcctgaaaa acaactgaat attgaactgg taagcgcgcc
     2821 aaaagacacc aatgaaaatg tctttaaagc cagtaaacaa accattggtt tcagtgatgt
     2881 aacgccggtc attacaacca gggaaaccga tgacaaaata acatggtcac tgacaggcta
     2941 taacacggta gcaaacaagg aagcaacccg gaatgccgcc gccctgttct ctgttgacta
     3001 taaagcgttt ctgaacgagg tcaacaacct gaacaaacgt atgggtgacc tgcgtgatat
     3061 caacggcgaa gccggtgcat gggcacgcat catgagcggt accggctctg ccagtggtgg
     3121 tttcagtgac aactacacgc acgttcaggt cggggtcgac aaaaaacacg agctggacgg
     3181 actggatttg tttaccggtt tcactgtcac acacactgac agcagtgcct ccgccgatgt
     3241 tttcagtggt aaaacgaagt ctgtgggggc tggcctgtat gcttccgcca tgtttgattc
     3301 cggtgcctat atcgacctga ttggcaagta tgttcaccat gataatgagt acactgcaac
     3361 ctttgccgga ctcggaaccc gtgattacag cacgcattca tggtatgccg gtgcagaagc
     3421 gggctaccgc tatcatgtca ctgaggatgc ctggattgag ccacaggctg agctggttta
     3481 cggttctgta tccggtaaac agtttgcatg gaaggaccag ggaatgcatc tgtccatgaa
     3541 ggacaaggac tacaatccgc tgattggccg aacgggtgtg gatgtgggta aatccttctc
     3601 tggtaaggac tggaaagtga cagcccgtgc cggtctgggc taccagttcg acctgctggc
     3661 taacggcgaa accgtattgc gggatgcatc tggtgaaaaa cgcatcaaag gtgaaaagga
     3721 cagccgtatg ctgatgtccg ttggcctgaa tgcagaaatc agggataacg tccgctttgg
     3781 actggagttt gagaaatccg cctttggtaa gtacaacgtt gataatgcag tcaacgctaa
     3841 tttccgttac tcgttctgat actccgtatt caaaagggcc tgcgggccct ttacgctcag
     3901 tgcctgccgc cctgatatgt ctgagagaca tttccgggat gtcagtcgac tggtttactt
     3961 aactgcgatg actaaagctg gcagtaatgc cggctttttt acggccacat acgtcttcct
     4021 ctcatccgga actgtgatac aaatcctgca tacaggattg accgccggtt cctgcaaatg
     4081 gcacattttc agtaagatag cgaaggttac attcccccgt catgtgtggc acaagctgtc
     4141 accgggaaag aaacggaaag cacatgaaaa acatcaaaac tcacaccggc ctccttatcc
     4201 acaaagaaca aacgcggcgc gttcgtcttc atgaaacacc cactgcctgg tgccacacgc
     4261 accgggaatg ctacagcaaa acgaccggtc gccgctgcgg cagccctgac agtctcagca
     4321 ggctgattct gagcagtatg aggtagcctg agtttaacgg acactccttc ctgaaataga
     4381 atggcatcag aaggagctaa taatgagcag aaaaacccaa cgttactcta aagagttcaa
     4441 agccgaagct gtcagaacgg ttcttgaaaa tcaactttcg atcagtgaag gcgcttcccg
     4501 attatc
//
LOCUS       DAZUGK010000277         4452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZUGK010000277 DAZUGK010000000
VERSION     DAZUGK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.279"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..479)
                     /locus_tag="ACHY5W_004410"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="ACHY5W_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(713..1390)
                     /locus_tag="ACHY5W_004411"
     CDS             complement(713..1390)
                     /locus_tag="ACHY5W_004411"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4449165.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1588..3207
                     /locus_tag="ACHY5W_004412"
     CDS             1588..3207
                     /locus_tag="ACHY5W_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFS4449166.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            3204..>4452
                     /locus_tag="ACHY5W_004413"
     CDS             3204..>4452
                     /locus_tag="ACHY5W_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFS4449167.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLTGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLARVLTSKYAEHTPLYRQSEIYGRQ
                     GVELSRSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGR
                     LWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNG
                     GITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQR
                     KTKPLLKSLESWLREKMKTLSRHS"
BASE COUNT         1063 a         1164 c         1159 g         1066 t
ORIGIN      
        1 gatttatgaa gaattttact gttgtacagt accagatact cagccagttt cagattgaac
       61 atattcagct attcaaagag caattcttca ttaaactcaa taaattgttc cctcagtgtt
      121 cggttaaaat gctcacttat ggcatttatt ttcggcgtat agagataagt ccatacgaac
      181 ccgacggtgg cttcctgtag cgttttgtcg aattttcccg agaactcttt gcccatcatc
      241 agtgatgatc tggttgatac caacaaggaa caactgggct acacagcaga agaaatgcct
      301 gatgatatca cttttaagcg atagtacggc cagagagtag ttgctgtgtt cgtcgagcat
      361 ggtaatgaaa taacggtaca gctcgccatt ctgagctcaa tagtatccat gccactgagc
      421 ttacaggtct ttatcgggcg atagtgcttt attcttcggg gctttgtgga tgatttttcg
      481 aaatgttcat gatagttagg taaagctacc gcatatcatt tgcaaaatga cagatagata
      541 tacttcaatt tgaatcttaa caattagtat gggaatgctg tcaatcctct gtatgcgtcc
      601 accacatatt gaaatttttg ttctctaata tattccacgg gcgtcaagag tgtgaggcct
      661 gtgctgttat acagcaccag acgttgtccc ccatctttga cctgtctctt gatcagatct
      721 cctgatcaag agacttcatc accaggtaac cctcagccat atcctgatgt ctgaaccagc
      781 catcccacat aactacccaa ccggggctgc ttgtgcgttt gctgtcatgc catcgcccca
      841 gtttagccag tttcaaacag gcccatttca gggtcggtgt ttgcgctgga agctgctttc
      901 cttccagctt aacccacagt agtttccact ctgtcggcgt cagtattttc tcacagctgt
      961 cattttgtgt ttcttcactg atacttccct gccgcaggcc cagcacccgc accgcgataa
     1021 ccaccatgcg ctcaaggtta tcccgggtct gcattcgcag cgattccaca catgtaccac
     1081 cacttttcca cgctttgtgg tattcctcta tcagccagcg ttgctcgtaa tggctgacga
     1141 tacgtcgcgc atcggcggca ctcgccactt tttctgatgt cagcggatac cagcaggctc
     1201 cgtcctctcc tgctccccgc aacagacata cgtgagcgag agcgcctggc cgctgttgtc
     1261 gggatttttt atgctgactt cgctgtaact gatgagcatc cgggcctggc gggctgcccg
     1321 cccgcctttt tgcatcacat tcagcgtgtg gcttcccgcg gctgacttta taatgcaggt
     1381 aatgccagat atccgcttcc cggtcacaga cagtgattac ctgtttctgt atctccccca
     1441 gccgttcggc catgcgctcc gaagcctgct gccagcggta actttctttt tctgtcacga
     1501 acggtgcaat agtggttctt ccgcaacttg ggtggaagaa aatggatgaa actttctggt
     1561 gaacaaagat ctaagctgtt ttaagtcatg ggcaactcaa tgcactctct taagacactt
     1621 ctacagttac cttgcggatg gcgatgcagt cgacaaatta ttagctctga cggtataacc
     1681 ctccatctcc acggaaaacg caaaaaagca caatgtcctg aatgctttaa gcgtagcgac
     1741 tctgttcata gttgtcgtcg gcgccggata cagcatctac cctgctcagg acatacgcta
     1801 tggcttgtat tttccgtccg ccactggtac tgccgtaacc cggcttgttc acgtaaaatt
     1861 tttgccgggt cgcttgctcc cttcgccggt tcacaccaac agtcttcaca ggcgttacaa
     1921 aatttacaac gtcaactggg attaatcgcc ggaggtgagg ccgggaaacg ggcagcaacg
     1981 gcagtggggc tccgttgcag tgctgatact ctccttcgca gggttatcaa taccccggag
     2041 acgaaacagt caggcgcgcc tcatgtcggt attgatgagt gggcgtggca tcggggccac
     2101 cgttacggta cgttaatcgt caatcttgat actcaccgtc ccctcgtcct gcttcccggc
     2161 cgtgatcagc gtacgctggc gacctggttc agaaaatatc cggaaataca aattgtctcg
     2221 cgtgatcgca gtggagtcta tgcgacagca gcacgtgaag gtgcacctca ggccagacag
     2281 gtggccgatc gatggcatct gctaaaaaat attggcgatg cgcttgaacg aatgatgtac
     2341 agacatatac ctctgatacg tcttgttgcc agtgagttgt caccaaagaa atcacctgat
     2401 ccagaaccat ctgtgcctgc agcatcgctc cgtcgtccgg aacgcctaaa acaacaaacc
     2461 cgcaaaaaac ggcatcagcg ttggacagag gttatggccc tgcataacaa ggaatgtagt
     2521 ttcagggaaa tatcccgtat tacaggcctg tcgcgtgtga cagtcagtcg ctgggtgcgt
     2581 tcaggaacat tccctgaaat gtcaacccga cctccaaagc gagggcttct ggacccatgg
     2641 agggagtggt taaaagagca acgagaaagt agtaattata acgccagccg gatatggcgg
     2701 gaaatggtgg cccggggatt tacaggcagt gaaaccatcg tcagggatgc ggttgccaaa
     2761 tggcgtaaag gctggaaccc accggttact actgccgtca gacttccttc agtgtcccgg
     2821 gtaagccgct ggttgatgcc ctggagaata accagggatg aagaaaatta tgcttcccga
     2881 tttattagtc tgatgtgtga aaaagaacct gagctgaaaa tagcgcagca actggcactc
     2941 gagttctacc gtattctgaa aacccaaaat aaatcacagc taagcagttg gttcaatcga
     3001 gtccacgaaa gcggttcagc agaatttcgg cgcgtggcgg cggggatgga agctgatgct
     3061 gcagctatat gtgaggcaat cagtagtcgc tggagtaatg gtgttgtcga aggtcatgta
     3121 aatcgcctga agatgctgaa acgccagatg tatggtcgag caggatttga actactcagg
     3181 cagagggtaa tgagtccact ggcatgaaaa tctccaccca ggttgcggaa gagccacttt
     3241 ccgtcgttga cactgacgac atcttcctgc tgaaacagcg cctggccgaa caggaagcgc
     3301 tgatccacgc cctgcaggaa aagctgagca accgggagcg cgaaatagac catctgcagg
     3361 cgcagctgga taaactccgc cggatgaact tcggcagtcg ttccgaaaaa gtctcccgcc
     3421 gtatcgcaca aatggaagcc gatctgaacc ggcttcataa agagagcgat acgctgactg
     3481 gtagggtgta tgacccggca gtacagcgtc cgttgcgtca gacccgcacc cgtaagccgt
     3541 tccctgaatc actaccccgt gacgaaaagc gactgttgcc tgcggcgccg tgctgcccga
     3601 actgcggcgg ttcactgagc tatctgggcg aggataccac cgaacagctg gagttgatgc
     3661 gtagcgcctt tcgggttatc cggacggtac gggaaaaaca tgcctgtact cagtgcgatg
     3721 ccatcgtgca ggcacctgca ccttcgcggc ccatcgagcg gggtatcgcc agaccggggc
     3781 tgctggcccg tgtgctgacc tcgaagtatg cagagcacac cccgctgtat cgccagtcag
     3841 aaatatacgg ccggcaaggt gtggagctga gccgttcact gctgtcgggc tgggtggatg
     3901 catgctgccg gctgctgtct ccgctggaag aggcgcttca tggctatgtc atgactgacg
     3961 gcaaactcca tgccgatgat accccggtcc aggtactgct gccgggtaat aagaagacga
     4021 agaccgggcg gttgtgggcg tatgttcgtg atgaccgcaa tgccgggtca gcgttggcac
     4081 ctgcagtgtg gttcgcttac agcccggaca gaaaaggcat ccatccgcag actcatcttg
     4141 cttgcttcag cggtgtgctg caagcggatg cgtacgccgg gttcaacgag ctgtatcgca
     4201 atggtgggat aacggaagct gcctgctggg ctcatgcccg ccgaaagatc cacgatgtgc
     4261 acgtccgcat cccgtcagca ctgacggaag aagccctgga gcagatcggt cagttgtacg
     4321 ccatagaggc ggatataagg ggaatgccgg cagaacagcg gcttgctgaa cgtcagcgaa
     4381 aaacgaaacc gctgttgaaa tccctggaaa gctggttgcg tgaaaagatg aagaccctgt
     4441 cgcgacactc ag
//
LOCUS       DAZUGK010000278         4422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZUGK010000278 DAZUGK010000000
VERSION     DAZUGK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.280"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..760
                     /locus_tag="ACHY5W_004414"
     CDS             20..760
                     /locus_tag="ACHY5W_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFS4449168.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(890..2860)
                     /locus_tag="ACHY5W_004415"
     CDS             complement(890..2860)
                     /locus_tag="ACHY5W_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFS4449169.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     AVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQTHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2871..4271)
                     /locus_tag="ACHY5W_004416"
     CDS             complement(2871..4271)
                     /locus_tag="ACHY5W_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4449170.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVSYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         1059 a         1146 c         1196 g         1021 t
ORIGIN      
        1 ttaaaatgga ataccggaca tgttaattaa ccgcaatatt gtggcgttat ttgcgttgcc
       61 ttttatggca agcgcaactg cttctgaatt atccattggt gccggtgcgg cttataatga
      121 atcgccttat cgcggttata atgaaaatac aaaggcaatt cccctgatta gttatgaagg
      181 tgattctttt tatgttcgtc agaccacgtt aggttttatt ctgtcgcaaa gtgaaaaaaa
      241 tgaacttagc ctgaccgcat cctggatgcc gctggaattt gaccctgccg ataatgacga
      301 ttatgccatg caacagcttg ataagcgtga tagtacggct atggcggggg ttgcctggta
      361 tcaccacgag cgttggggaa ccgtgaaagc ctctgcagct gcggacgttc tggataacag
      421 caacggctgg gtgggggagc tatcggtatt ccacaaaatg cagataggtc gtctgtcgct
      481 gacacctgcg ctgggcgttc tctattatga cgagaatttc agtgactatt actatggcat
      541 ttcagagagt gagtcccgtc gtagcggtct ggcaagttat tccgcgcagg atgcctgggt
      601 gccctatgtc agcctgacgg caaaataccc gataggagag cacgtcgtat tgatggcgag
      661 cgcaggatac agcgagctgc cggaagagat taccgacagc ccgatgattg atcgtaatga
      721 gagttttacc tttgtcaccg gcgtgagctg gcgtttttaa ttcaccggtg gatgtcggtg
      781 cggctggcct acgctctcgc accgacttgt atgcctgata agacgtgtta agcgtcgcat
      841 caggcaatgt gctccttacg ttggatgcgg cgcgagtgcc ttatccaacc tacggatggc
      901 aatgcttttc ctcattcacc cagatccgca tttcgccttc accccggttg gcccagctaa
      961 accacgggat aaaggtcaga gtttgcgtct ggcgtttggc tggcgcgctg tcgtaatgcc
     1021 acagcggttg ctgctctgga ttgctctgtt cataccggta acccggtgcc tggattaaga
     1081 tcttatggct aaacaatccc ttgccttcaa atgtcgtaaa tggcgcatcg gtgggcagcc
     1141 acagattatg cagtgactcg ccgttgtcgg cctgttccag gcaatacacc agcgggccgc
     1201 gctgaatcgc cacttttccg gcgacgtgac gcaccagcgg gttaccgtaa acgcggcgta
     1261 ccggcatcgg caaagtcaga ttcagcgtat cgccctcctg ccattcgcgg gtaatgtgca
     1321 aatacccttt acgaatatcc tgctcgacct cttccccatt caatatgatc tgcggctgtg
     1381 tgcaccagtc cggcagacgt aaagccagcg tatgacgtac cggctggggc gattcaaccg
     1441 caatcgtcac ctgctcctgc cacggatagt tcccgctaac ccgcaggcgc agcgtgccat
     1501 tttctaccgg cacttccatg ctgtttcctg cgtatatgtt gatatacaac gcatcttcac
     1561 gcggcgtgta gagataatga ccaatcgagg tcagcacgcg ggcgatattt ggcggacaac
     1621 aagcgcagcc aaaccaacgc tggcggatcg gtttaacgtg atcgtagata tggttgaatt
     1681 tcagcgattt tggatgtact tccagcggat tcacatagaa gaaatgtttg ccatccagcg
     1741 ccatgccgcc gagcacggtg ttgtacagtg cgcgctccat cacatcggca tattgactgt
     1801 cgccttccat ttccagcatt cgtcgggcaa acatcatcag gccgatggag gcgcaacttt
     1861 cagcgtatac cgtgtcattc ggcagatcgt aatcgctagt gaacgcttcg ccgctgcttt
     1921 gcgagccaat gccgccggta atatataact gacgctgggc catattgttc cacagccgca
     1981 ggcagtcctg acgcttgctg tcatcgtgac ttaaacgcgc gagatgcgcg acgccggtca
     2041 tcagatagac aaaacgtacc gcgtgaccga tggcggtttg ctgttgcgca aggggcaaat
     2101 gtgtctggct gtaggctttg tctttcacca tccacgccgg gccgtaggtg tgccagtgcg
     2161 atgtctgccc gcgcttttca tattcctggt cgtaatagtg cggttgcgca ccacgctgtt
     2221 cgacaaaata gttcgtcagc gccaggtagc gcggctcttc agtcacttca tacaggcgca
     2281 ttagtgccag ttcaatttcc gggtgaccag gataaccgtg taacttactt tcatctggac
     2341 caaatacgct gtcgatatga tcggccaggc ggcaaacggc ccccagcaag cgccgttttc
     2401 ccgtggcctg gaagaaggcg actccggctt caatcagatg acctgcgcag taaagttcat
     2461 gacactccgc cagattgctc cagcgttctt cgggtgcttt taccgtaaag taagtattga
     2521 gatagccgtc ttcgcattgg gcggaggcga tcagttcgat tacctcgtcg gcggtttttt
     2581 ccagttcggc gtccggcttc tggcacagcg accaggctac cgcttccagc catttggcga
     2641 cgtcgctgtc ctgaaacacc atcccgtaaa attcaccctc ctgaagtccg gcggcaatgc
     2701 gaaagttttc aatcgcatgg ctgggttccg cttctgggat acgatcgttc aaggcatccc
     2761 actgataagg aatcaccacg tcgcggacca gttgttggta ctgaccgagg aacggatcgc
     2821 tgaccgtcag tttatgcaga tcgacttccg aaatgttcat cttgtgctcc ttaaggctgt
     2881 acatgacgct gacgcaggtc ggtagctatg cgtgacatca taggattatt gagtttgcaa
     2941 aagcggatcg cccctgccag cagcaggtgg aatagcgcag gcagtaatgt ttccatcgcc
     3001 gcgatcccgc caagcgaact ggcggtctgg ttttccacgc caggctggta ggcgacaaaa
     3061 ataaacagca ggctgatgat cccggcgctg gaggcccagg ccagcttgat gaaaaacaga
     3121 ttgaaggcga agttcatgcc ggaagaacgt acgcgggttt tccactcgcc gtagtcatcg
     3181 gcaaaggcca tcaatgagaa atgcagtggc aatgtgaagc caagaattac gccattaccg
     3241 aggatcaccg ccagccacag cgtttgataa gccgggccgg agggcaggaa ccacatcaac
     3301 accgccagcg cagcgaggag caggttggtg tagtagtaaa ttttgacggt atcgaaacgc
     3361 cgcgttaacg ggctgacaat caccgagccg ataatggagg cgaaggtgac catggtgaag
     3421 aacagcgacg tgtagcccgt actgccttgt aagacgtagg taatgaagta catataccca
     3481 ccgccgcgaa tgttaaagac gttaatcagc aggaaagaca tgaccagcat cagcagcagt
     3541 tggtcgttgt tccgcagccc ggcaagatgt tcgcgcaggg taaatttccc cattgtggag
     3601 agtggcaccc gttcacgaac ccagaaaaaa cagcacagga acatcaccac ggcaatggcg
     3661 cacagcacgc cgacgcccaa ttgatagccg cgcgcggcat taccctgacc gaagagatct
     3721 accatccacg gtaagcctac tgaaaccaga aatcccgcta caccacagag aacgaatcgc
     3781 caggactggc aggagatcac ttcattgtgg cgggtggtca tggtgttgat cagcgcgcag
     3841 tacggtacgt tgatggcggt ataactgact gaaaggagca ggtaagtacc gaacgcccag
     3901 gcgattttta ctcccatgct ggcatctggc acggtgaacg tcaggatgcc gataatgccg
     3961 atgggaatgg caatccatag ctgccacgga cgaaagcgcc cccagcgact ttgcgtccgg
     4021 tcggcaatga cgcccatcac cgggtcggag atggcatcaa acacgcgcag tgcaataaac
     4081 agcgtgccaa ccagcgccgg agttaatcca aaaacgtcgg tgtagaaaaa ggtgagaaaa
     4141 ttcatgatca ggcaagttat tacggtgccg cctgcgtcac ccaggccgta gcctattttt
     4201 tcccgtacag ataacgcatc gtcaatttta cctttagcga tatcggcggt agtaatcggt
     4261 gtagaggtca tagatattcc tcgtgaatag ggagcaattt ttagttttat tgaaatgacc
     4321 tgattaattc tgccgtgaac gggaatatca acaaggcgag aaaagagtaa aaaaaggtga
     4381 cgattccgac ctgaatttaa aagtgtgaaa tggatcaggt ga
//
LOCUS       DAZUGK010000279         4398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZUGK010000279 DAZUGK010000000
VERSION     DAZUGK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.281"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1065
                     /locus_tag="ACHY5W_004417"
     CDS             <1..1065
                     /locus_tag="ACHY5W_004417"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4449171.1"
                     /translation="GQELQALAQMTALQKQGHSVLLACREKSKIAPEARKRGHDVTFI
                     PFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLICCHRFSIVRQKTYIT
                     RKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDWPALHNEAMRPLPLH
                     IHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFRWLVVGAGREEYEAR
                     LRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGMVLAEASVSGVPVIA
                     SETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMMAASAREDIEYRFDI
                     NRTAQIIVSLASQAKGKCNR"
     gene            1159..2880
                     /gene="cptA"
                     /locus_tag="ACHY5W_004418"
     CDS             1159..2880
                     /gene="cptA"
                     /locus_tag="ACHY5W_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFS4449172.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            2954..3952
                     /gene="lpxM"
                     /locus_tag="ACHY5W_004419"
                     /gene_synonym="msbB"
     CDS             2954..3952
                     /gene="lpxM"
                     /locus_tag="ACHY5W_004419"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFS4449173.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            4005..4193
                     /locus_tag="ACHY5W_004420"
                     /pseudo
     CDS             4005..4193
                     /locus_tag="ACHY5W_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4256..>4398)
                     /locus_tag="ACHY5W_004421"
     CDS             complement(4256..>4398)
                     /locus_tag="ACHY5W_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFS4449174.1"
                     /translation="YSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALP
                     AE"
BASE COUNT         1088 a         1010 c         1138 g         1162 t
ORIGIN      
        1 ggccaggagc tgcaggcact ggcccagatg actgctcttc aaaagcaggg acattccgtt
       61 ttacttgcct gcagggaaaa aagtaaaatt gctccggaag ccagaaaaag agggcatgat
      121 gtcactttta ttcctttcag gaacagcctt caccttcctt ccatcctcag acttcgacgg
      181 attatcgggg aatttaaacc tgatctggtc atctgccata gtggccatga cagtaatata
      241 gcaggactga gccgtctgat atgctgtcac cgcttcagta ttgtcagaca aaaaacctac
      301 atcacccgta agacccgaac gttttccctg aattatctgt gtgatttcat tgttgtgcct
      361 agctcagcca tgatggcgca tctgatggcg gaaggtgtca ggacaccggt aacggttatt
      421 ccaccgggtt tcgactggcc cgcattgcat aatgaagcca tgcgaccgtt acccctgcat
      481 atccacgcct gggctgcatc agcagacaat gttccgctta tcgttcaggt ggggatgcta
      541 cgtcccgaaa aaggacatga atttatgctg cgtgtattgt atcagctgaa gatggaaggg
      601 aaatcatttc gctggctcgt ggtgggcgcc gggcgtgaag aatacgaagc acgtctgcgt
      661 cagcagacag aacacctggg aatgagcgga gatgtgttaa tggcaggtgc ccttttccca
      721 gcgttaccgg tctaccggat tgccagtgtg gtggtcatgc cttcggaaaa tgaagcgttt
      781 ggtatggtac tggctgaagc gtcagtctct ggcgtgcccg ttatcgccag tgagacgggc
      841 gggatccctg atgtcattca gaagaatgtg acagggacgc tgttacccgt gggggatgtg
      901 tctgcatgga ccggcgcact gcgcgatttt ttatcccgac cggaacgctt ccggatgatg
      961 gcagccagcg ccagagaaga tattgagtac cggtttgata ttaacagaac ggcacagatc
     1021 attgtgtctc tggctagtca ggctaagggg aaatgtaaca gataacggcg gatcctcttt
     1081 tcagcccgaa ttttgttcag cggttttccg gcaagtaacg gagtaattta tcacattgtt
     1141 taaatacggg gacggtcaat gcatttaaat accggacaga acaggcctac tttcagctgg
     1201 agtgcgctgg gatgggcaat tttttatttt ggcttttttt ccactctcct gcaggtcatc
     1261 atcttcagca gtgggtacag cggaacgaat ggaatacggg actcactgtt attcagttgt
     1321 ctgtggttga tcccggtgtt tctctatcct gatcggataa aaataattgc agctgttgtc
     1381 ggtttcattc tctggggcac gtcgctggca gcactttgtt attattttct ctatggtcat
     1441 gaattctctc aaagtgttct tttcgttatg tttgaaacga atgccagaga ggctggtgaa
     1501 tatttcagcc agtatttcag ccttaaactg ttgcttatct cgctggtata tactgcggtg
     1561 tccgtttttc tgtggacacg tctgcgtcct gtatatattc ctttgccatg gcggagaatt
     1621 gtctctttcc tgctgcttta tgctctgctt ctgcatccgg ttgttctgaa atcgttaatc
     1681 agacaggagc cgctgaatga tactctaggc aaactggcat cccgaatgga gcctgctgcc
     1741 ccctggcagt ttgtatccag ttattatcag taccatcagc aactgaatgc actgacaacc
     1801 ttcctgaatg aaaatagcgc actgccacca ctgggtaatc tcagggatga atcaggggag
     1861 agaccacgca cactggtcct ggtgattggt gagtcgacac agcgcgaacg catgagctta
     1921 tacgggtatc tacgtgaaac gacgccggag ctggatgcac tgcgtaaaac cgatccgggt
     1981 cttactgtgt ttaataatgt ggtggcatcg cgtccgtaca ccattgaagc attgcaacag
     2041 gcccttactt tcgccaacga aaagaatcct gatctgtatc tgacgcagcc gtcgctgatg
     2101 aacatgatga agcaggcagg ctataaaaca ttctggatta ccaaccagca gacaatcaca
     2161 gcccgtaaca ccatgctcac tgtattttcg cgccagacgg acaggcagta ctacatgaat
     2221 cagcagcgaa cacaaagtgc gcgtgaatat gacactaacg tgctgaagcc gttccgggaa
     2281 gtgctgaatg accctgcacc aaagaaactg atcatcgtgc atctgctggg tacgcacatt
     2341 aagtataaat accgttatcc ggaaggtcag gggcggtttg atggcattac agggcatatt
     2401 cccactggat taaatgcgaa agagctggaa gtgtataacg attacgataa tgccaatctg
     2461 tttaacgatc atgtggtggc cagtctgata aaggacttca gagcaactgc gccggacggc
     2521 tttctgcttt atttttcaga tcatggtgaa gaagtatatg atactccgcc atataaaacg
     2581 cagggacgga atgaagataa ccccacacgt cccatgtaca ctgttccgtt cctgctgtgg
     2641 acctcggaaa agtggcatgc tgcacatccg cgagattttt cgcagtatgt tgaccgcaaa
     2701 tacagtctgg ctgaactgat ccacacctgg tcagatttgg cgggactgac atacgatggt
     2761 tacgatccga cccgttctct ggtgaatccg caattcaggg aaactacccg ctggattgga
     2821 aatccgtata aaaagaatgg gctcactgat ttcgacactc ttccgtatgg tgagccatag
     2881 tattttctta tcttcggttc cgttctgaag gcaggaggat gtgttttcct ctgctgtccg
     2941 gtggactcca gggatggtac caccttctgc tgtactctgt tatcataatg agataagcag
     3001 gcagataccg gtaaatatga aaaatatcag aactgagttt attccccgct ttaaccttac
     3061 attatgcttc ccccgttact ggatgacgtg gacaggtatc ggtattatct gtgtgtttgc
     3121 aatggtcccc cccgcactga gagatccatt gttgggaaaa ttgggcatgc tggttgggcg
     3181 tctggggaaa agcgcacgtc aacgggcctt gattaattta tcgctttgtt ttccggagta
     3241 cagtgataaa gaaaaggaga atatcgttga tgccatgttt gcaacagcct ccatggccgt
     3301 tgtgcttatg gctgaattag cactgtccgg gcctgataaa atttcgcatc gtatacgctg
     3361 gaatggtctg gagattgtgg agaagatggc acagaacaat gaaaaggtca tttttcttgt
     3421 gccacatgcc tggggagtgg atattcccgc aatgctgata gctgcgtcgg gcagaaaaat
     3481 ggcagcgatg tttcataacc agagaaatcc ggtggtggat tatgtctgga acagtgttcg
     3541 tcgtcgtttc ggaggaaaac tgcacgcacg taatgacggt attgcctcct ttgtccgctc
     3601 agtccggcag ggatactggg gatattatct tcctgatcag gatcatggcc cggaattcag
     3661 tgagtttgca gatttttttg cgacgtataa agcaacttta ccggttatcg gacggctcag
     3721 caggatcagt ggtgcacgga taataccgct tttccctgtt tatgacggaa agacccatca
     3781 tctgactatt catgtcagcc cgccacttgc tatacgacag aagagtgatg ctcatattgc
     3841 gcgacagata aacgaagtgg ttgaaaattt cgttcgtcca catcctgagc agtatacgtg
     3901 gatcctcaag ttgctgaaaa cgcgtaaaga aggggaagag gatccttact gaggcaggtt
     3961 tttcaggata atgagcttac ccagcaatag tggacacacg ctaagtgagt aaactctcag
     4021 tcagaggtgg ctcatatgac aaaaaaacag taccaaccag taaaaaaccc cgtaaatagc
     4081 attcgcctga atttcgcagt gaagccctga agcttgctga acgcatcggt gttgctgtcg
     4141 cagcccgtgt gctcagtctg tatgaatcac agcttgtaag cggctcgtca gaactgtatt
     4201 gatatttact gagcggtcgt aagcgtacag cgagggccgt attgacgggg atgtgttatt
     4261 cagctggcag tgctatgcgc cacggaagca gttcgctgac ccggttgacc ggccagtctg
     4321 ctatgacgcc aggcacatgg cgaaggtagc tttctggatc cacgtcattc agtttgcacg
     4381 tcccgatcag gctgtaca
//
LOCUS       DAZUGK010000280         4336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZUGK010000280 DAZUGK010000000
VERSION     DAZUGK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.282"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHY5W_004422"
     CDS             <1..108
                     /locus_tag="ACHY5W_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS4449175.1"
                     /translation="YRAVRDCWRRQIDKRAAEKISASQSAGSKPEEPLI"
     gene            149..421
                     /locus_tag="ACHY5W_004423"
     CDS             149..421
                     /locus_tag="ACHY5W_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS4449176.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            498..713
                     /locus_tag="ACHY5W_004424"
     CDS             498..713
                     /locus_tag="ACHY5W_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449177.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            717..1358
                     /locus_tag="ACHY5W_004425"
     CDS             717..1358
                     /locus_tag="ACHY5W_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449178.1"
                     /translation="MNHEEMNQRISCLENEITELNKKLSVLMVSEDEKKRRDEQEAAF
                     YDDCIKIARRTFAKILQEKFLPTALSEKYSITVKSAGEEGNKRYFIASAPDKDQEWGD
                     NRPSFIVTSDDWNITIREDGKVTPASHQHSEALIEFAIDYLKNNKKQGLMKRIGRCMG
                     YLQVAAEIEALASGADKDAVVREALLRDFDNPPFKKVPAYWFHPGLTYLKGRI"
     gene            complement(1368..1682)
                     /locus_tag="ACHY5W_004426"
     CDS             complement(1368..1682)
                     /locus_tag="ACHY5W_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449179.1"
                     /translation="MYNDEHKYTACMQAMNEQFKSAFLKLIQQNHEAVKSIQAEPYGH
                     LTPPTLDIMSRILTPAMLLRLKDNINDWLNEELNYLECEWDHHYAKSQKERIFRRLSG
                     NR"
     gene            1811..2344
                     /locus_tag="ACHY5W_004427"
     CDS             1811..2344
                     /locus_tag="ACHY5W_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449180.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2500..2682
                     /locus_tag="ACHY5W_004428"
     CDS             2500..2682
                     /locus_tag="ACHY5W_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449181.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            2695..2815
                     /locus_tag="ACHY5W_004429"
                     /pseudo
     CDS             2695..2815
                     /locus_tag="ACHY5W_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3043..3228
                     /locus_tag="ACHY5W_004430"
     CDS             3043..3228
                     /locus_tag="ACHY5W_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4449182.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            3314..3538
                     /locus_tag="ACHY5W_004431"
     CDS             3314..3538
                     /locus_tag="ACHY5W_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449183.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQRGRVLIEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMASAALQPVC"
     gene            3957..>4336
                     /locus_tag="ACHY5W_004432"
     CDS             3957..>4336
                     /locus_tag="ACHY5W_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFS4449184.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWT"
BASE COUNT         1134 a          936 c         1209 g         1057 t
ORIGIN      
        1 tacagggcag tgcgcgattg ctggcgcagg cagattgaca aaagggccgc agagaaaatc
       61 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
      121 taatcacatc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
      181 agcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac agaaacttcg
      241 ccataacctg gaagcgcgtc ttgatgcaga ggttgatgcc attctggatg agctgcttgg
      301 tgtacaggca gagccaccga ctgaagcggg agataccacc gcagagagcg gtgaagttca
      361 gcctgaatca ccggtcgccg atgcgactga acctcaaccc gaatcggtca tgatgctgta
      421 acggggagtc agggccatca gtaaacagct gctggccttt ttcatgttgt gagcttccgg
      481 ataacgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca
      541 cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc tctccgtccc
      601 agtgggtggc aataggcgtg ctggggagtc tgctgtttgg tctgctgacg tacctgacga
      661 acctgtattt caaaatcaga gaagaccggc gtaaggcggc gcggggagag tagacgatga
      721 accatgaaga aatgaatcag cgcataagtt gcctggaaaa tgaaatcact gaactgaata
      781 aaaaactgtc ggtgctgatg gtttctgaag atgaaaaaaa acgccgcgat gagcaggaag
      841 cagcgtttta cgatgattgc atcaaaattg ctcgcaggac ctttgcgaag attttgcagg
      901 aaaagttttt accgaccgca ttgtcagaaa agtactccat tacagttaaa agtgccggag
      961 aggaaggcaa taaacgttat tttattgcgt ctgcaccgga taaagaccag gaatgggggg
     1021 ataatcggcc atcttttatt gtgacaagcg atgactggaa tatcacgatc cgtgaagatg
     1081 gaaaagtaac accagcatcg caccagcaca gtgaggcgct cattgaattt gccattgatt
     1141 acctgaagaa caataaaaag cagggactaa tgaagcgcat tggtcgttgc atgggatatc
     1201 tgcaggtagc tgctgagatt gaagcgctgg ccagtggtgc ggacaaggat gcagttgtgc
     1261 gggaggctct tcttcgtgat tttgataatc cgccctttaa aaaagtgccg gcttactggt
     1321 ttcatccagg actgacttat cttaaaggac gtatataagc tggctcgtta tctgttgccg
     1381 gataatcgac ggaagatgcg ttctttttgt gatttggcgt aatgatgatc ccactcacat
     1441 tcaaggtagt ttaattcttc gtttaaccag tcatttatat tgtctttcag acgtagaagc
     1501 atggctggtg ttaatattct ggacataatg tcgagagttg gtggtgtgag gtgtccatac
     1561 ggctcagcct gaatggattt tactgcttcg tggttttgct gaatgagttt aaggaatgct
     1621 gatttaaatt gttcattcat ggcctgcatg caggcggtat atttatgttc atcgttatac
     1681 attgctaaat cctcgacggg aattgtcaga tatatttcag ccatcaggta aaacaccagt
     1741 gcccaccact ggcgggctga agacttaaca tatccaggga ttcggaaccg ataaatcctg
     1801 ataaatatcc atgaacacca aaatcaaata cggtctgtcg gctgccgttc tggcgctgat
     1861 tgccgcaggt gcgcctgcgc ctgaaatcct cgaccagttt ctggatgaaa aggaaggtaa
     1921 ccacaccacg gcataccgtg atggtgcggg gatctggacc atctgccgtg gagccacccg
     1981 ggtggatggt aagcctgtta ttcctggcat gaagctgtca aaggaaaaat gcgaccgggt
     2041 taacgccatt gaacgtgata aggcgctggc atgggtggcg aaaaacatca aagtgccact
     2101 gactgaaccc cagaaagcgg ggatcgcgtc attctgcccg tataacattg gccccggtaa
     2161 gtgtttcccg tcgacgtttt ataaacgaat taatgcaggc gatcgcaggg gagcgtgtga
     2221 agcgattcgc tggtggatta aggatggtgg cagagactgc cgtatccgct caaacaactg
     2281 ttacggtcag atatcccgtc gtgaccagga gagcgcgctg gcgtgctggg gaatcgacag
     2341 ataagcagaa tattttgctg aaaaatgacg ttgaccaacg cggacggata acacgaaatc
     2401 ctgcgaactg gcaaaaccta agtgaataaa agtaaaaccc cgtttgttgg cagcaagcgg
     2461 ggttttgtgt ttctgacctt ggataaggca agggagaaca tggaaaagta taaacgaatt
     2521 ctgttgaggt tgactatgaa aaacggcctt gaactgaaag cgcctgtaac tgatgacatc
     2581 agcagagcgc tggcttttgc tattaagtgg gtggcggtcg gtattgctgt gtctccgatg
     2641 ctgtatgggc tggcaaaact ggtcattgcg ttgaaatcgt gaagaggatt aagcatgtca
     2701 gacaagctca taacgctggc gaagatcctc tgtgtaatcg tcggcatttc attttcacta
     2761 atgctggttg ctcttaggct ggatgatgtt gtcttcgtcg gggatgctgg ggtgaggggg
     2821 atatgaaccg tgttctgtgt gtggtgatta ttgtcctgct ggtagcctgt ggtgcgcttg
     2881 gtctggggct gaatcattac cgcgataacg ccatcaccta caaagcgcag cgcgataaaa
     2941 aagtcagaga gctggagctg gcgaacgcga caattactga tatgcagata cgccagcgtg
     3001 atgtcgctgc gctcgatgca aaatactcga aggaattagc cgatgcgaga gctgaaaatg
     3061 aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcggatcaac gccacctgcc
     3121 ccggtaccgt gcgtgaagcc accggcacct ccggcgtgga taatgcaacc ggcccccgac
     3181 tggcagacac cgctgaacgg gattatttca tcctcagaga acggttgatg atgatgcaga
     3241 agcagctgga aggggcgcag gaatatatcc gcactcagtg tattaactag tatttttgtt
     3301 atccggagaa tgcatgaaga aattactggt aaccgtaaag ccttttcagg gaacaattcc
     3361 gttccgtgtt ttgcagcgtg gtcgtgttct tattgaaggt tcgttcagtg gtaaatgtac
     3421 gcaattacac tcccggacct ttcaggtgaa tgccacgaat gaagagctaa ccgtggagtg
     3481 tacgatgaat gccgctaaat gccgcatggc atccgctgca ttacagccag tgtgttgagc
     3541 gaccttatta tccatgcgcg gtattgtcgc cgtattcccg tattaacaga gaccgcagcc
     3601 cgacagggcg actcctctgc gcgagtgtgc ggggataatc aaaaacgata cacaccgggg
     3661 tttaccgcgt taacggagcg cggcgttgtc ccctcatggt cgctggtccg gtgcgatggt
     3721 ggaagaaacc ggactacatt acaaatgata accattatca tttttcgggt cctcctggtg
     3781 tggtgggcct gaacacgggg cgggcggcgc ggaaaaaggc tcattttttg atttttatgg
     3841 caccatcacc accagtgtaa gttgttgata tatagaaaaa taaaaatttt tagtgtcgaa
     3901 tctggttgtt ttttgttcat cactggtgtg tgtttacata attttcaggg ggagttatgg
     3961 atcgtgaatt aaaaaatctg catctgaata tttcccaact ggccgcatta tccggtgctc
     4021 atcgacagac tgttgcggct cgggtaaaaa acataagccc agccggtggt catgagagca
     4081 atctcaaact gtaccgactg acagatatcc ttgccgagct gatgaaagct cctctgcctg
     4141 tagataacga ggaaatggat cctcatgcgc gtaaagcatg gtaccagtca gaacgtgacc
     4201 gactgaaatt tgagcaggaa actggtcagc ttgtgccagt cagtgatgtc aggcggtcct
     4261 tttctgtcgt ggtgaaagcg atagttcagg tactggaaac ctggcctgac cggctggaga
     4321 gggacagggg gtggac
//
LOCUS       DAZUGK010000281         4327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZUGK010000281 DAZUGK010000000
VERSION     DAZUGK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.283"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..102
                     /locus_tag="ACHY5W_004433"
     CDS             <1..102
                     /locus_tag="ACHY5W_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4449185.1"
                     /translation="ERIMDRMTMNGGRWVNFNWESWRPNVGQPGIAK"
     gene            125..868
                     /locus_tag="ACHY5W_004434"
     CDS             125..868
                     /locus_tag="ACHY5W_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4449186.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPH
                     PAGVALPVQEAATQEDIKTETVADIVQSLEKRVDNLVLPSLRQANRELRRAKSDIRKW
                     ERVCAALRELNKYRDIVAQLCREETSEQH"
     gene            855..1409
                     /locus_tag="ACHY5W_004435"
     CDS             855..1409
                     /locus_tag="ACHY5W_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS4449187.1"
                     /translation="MSNINYQALRMAAENATPGEWCSDDYGLIADAGLNANYYIASCS
                     GPDNRANKRFIAAANPATVLALLDEREAQSKRIAELETNLAALAAENAGLNKFIVQSC
                     YVFDGEQDELSDAYICAIDGRMPQTPATDAFLSDVRAGAFNDLCAAFVRHAKVAGLDD
                     ADTVTLKEVTEALLHCAEQIRAPE"
     gene            1505..1687
                     /locus_tag="ACHY5W_004436"
     CDS             1505..1687
                     /locus_tag="ACHY5W_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449188.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            1680..1856
                     /locus_tag="ACHY5W_004437"
     CDS             1680..1856
                     /locus_tag="ACHY5W_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449189.1"
                     /translation="MADFGSTKYNVSYEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            1853..2284
                     /locus_tag="ACHY5W_004438"
                     /pseudo
     CDS             1853..2284
                     /locus_tag="ACHY5W_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            2443..2934
                     /locus_tag="ACHY5W_004439"
     CDS             2443..2934
                     /locus_tag="ACHY5W_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFS4449190.1"
                     /translation="MVISPITLKAAQEFIAQHHRHNKPPVGHKFSIGLRNNAGELIGV
                     ATAGRPVARHLDDGLTLEVNRTCTTGERNANSALYGAVWRAAKAMGYQRCITYTQADE
                     SGASLRAAGFVRVKELPPRKSWAESSVALRSKRDPVGNGGIPRVLWEIRRMSTAGIRI
                     KGE"
     gene            2936..3580
                     /locus_tag="ACHY5W_004440"
     CDS             2936..3580
                     /locus_tag="ACHY5W_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFS4449191.1"
                     /translation="MSAITKERIKLFIKNPLDNGLTRGEQMELARIALASLEREQIRH
                     EHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLGEWADMQFLLWDAQRRAGISDA
                     EITAAMEDKLKINMKRQWPEPKDGEPRLHIKEPGNSPVTPDGWISCSDRMPEKGQNVL
                     ISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMKLPEPPQEVS"
     gene            3580..3975
                     /locus_tag="ACHY5W_004441"
     CDS             3580..3975
                     /locus_tag="ACHY5W_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449192.1"
                     /translation="MENEGDNIITLVQPKRDEEKLLNITVTGRKNYTQQSCKHRAIEV
                     HEQDRVILCLQCGCVVDPFQYVLRCANDGEAVVREIRQLHNRRDQLRESVASLEREEK
                     NTKARLRAARTAILYAENDLKNIEQKVNQ"
     gene            4260..>4327
                     /gene="hokD"
                     /locus_tag="ACHY5W_004442"
     CDS             4260..>4327
                     /gene="hokD"
                     /locus_tag="ACHY5W_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4449193.1"
                     /translation="MKQQKAMLIALIVICLTVIVTAL"
BASE COUNT         1281 a          921 c         1198 g          927 t
ORIGIN      
        1 gagcggatta tggatcgcat gaccatgaac ggcgggcgat gggtgaattt taactgggag
       61 agctggcgtc cgaatgttgg tcagccagga attgcgaagt aatttttacc gggaggaaat
      121 tttaatggag actgtttttg acgcactgaa agcaatggga aaagccacat ccatagaact
      181 tgctgcgcga cttgatatca gtcgtgaaga agtgctgaac gaactatggg aactgaaaaa
      241 ggctggtttt gttgataaaa gcgcgtacac ctggcgtgtg gctgataaca acgttcagca
      301 ggaacagcca gcgcaggcag aactgccgga agaaaccacc acagcaacag tagcgaaaat
      361 ctcagagtgc gatttaaccg caacgattga acaacgcgga ccacaaacgg ctgatgagct
      421 ggctacattg tttggtacca catcacgcaa agtggcttca acgctggcaa tggcaatcag
      481 caaaggtcgt ctgattcgcg taaatcaggg cggtaaattt cgttactgca taccgggcga
      541 taatttacca gcagagccga aagcagcatc ggtagcggaa actgatggta aggcctttcc
      601 tcatcccgca ggtgttgcat taccagtaca ggaggctgca acacaggaag atattaaaac
      661 agaaacggtg gcggacattg tgcagtcgct tgagaagcga gtggataatc tggttctgcc
      721 atcgctgcga caggcaaacc gcgaactgcg tcgggcgaaa agtgatatcc ggaaatggga
      781 gcgagtctgt gccgcgctgc gggagctgaa taaatatcgc gatattgtcg cccagctttg
      841 ccgggaggaa accagtgagc aacattaatt accaggcact gcgaatggcg gcagaaaatg
      901 caacccctgg cgaatggtgt tctgatgatt atgggttaat tgctgatgcc ggactgaacg
      961 caaattacta catagcatca tgctcaggac cagataatcg cgccaataag cgattcatag
     1021 cagcagccaa tccggctacc gtgctggcac tgttggatga acgggaagcc caaagcaaac
     1081 gcattgcaga gctggagact aatcttgctg cgcttgcggc ggagaatgcg gggctgaata
     1141 aatttatcgt acagagttgc tacgtgtttg atggcgagca ggatgaacta tctgatgcgt
     1201 atatctgcgc aatagacgga aggatgccgc aaaccccagc caccgacgct ttcttgtcgg
     1261 atgtgcgggc tggggcgttt aacgaccttt gcgcggcgtt tgtcaggcac gcaaaagttg
     1321 caggactgga tgatgccgat accgtaacgc ttaaggaagt gacagaagca ttgttgcatt
     1381 gtgcggaaca gattcgcgcg cctgaataat taaatttagt gttgtaaata aaatttaatc
     1441 cttaaccgga aggatttctg caccctcaga acagcaggag gccacccgaa agggtggtag
     1501 tgaaatgcga aagttcaaaa taattattga aacgggaata gctggtggag attttgagga
     1561 tgtattcgaa gtggacgatg acgcaacacc tgatgaaatt catgacgaag caaaagaaat
     1621 tttctttaac tactgcaatt actcatatca cgaaataaaa gacgaagagg aagaacaaaa
     1681 tggctgattt tggttcaact aaatataacg tcagttatga agaatggcat gaattgttaa
     1741 tggactatgc agagttacgc ggtggaagtg ctgctgatgc tgaagcctgg cgtgatgact
     1801 atgaagcagg aaaaactccg gtcgaagcat attgtgatga gtggggcgat gaatgagcga
     1861 aattaattat caggcgctac gtgaaaaggc agagaaagca actaaaggaa gctacatcgt
     1921 agggcataca tctgttaacc agcacggcaa tttaacagga gtttttgttt gccaaaaatg
     1981 gaaaggagaa cccggtggcg tgattgcgga atgtcatgtt aactgcctga ttgaatcaga
     2041 tgatcaggct tatgcaaacg ctgaattcat agcagcagcc aatccggcta ccgtgctggc
     2101 actgctggat gaacaggaaa gaaaccagca atacatcaaa cgccgcgacc aggagaacga
     2161 ggatattgcg ctaacggtag ggaaactgcg tgttgagctt gaggaagcaa aatcaaaact
     2221 caacgagcaa cgcgagtatt acgagggagt aatcgcggat ggaagtaagc gcatagcaga
     2281 actgtctgct agccacagca aattgcgcga cacaatggct ggcatccaca acacaatccg
     2341 aatggatggc ggctatacgc cactggcagc aatccttaac gctgctaaac gcgcatatga
     2401 agaatcagca agcgccgctg gcatcaagga ggagtctgag tgatggtcat ttcacctata
     2461 acgctgaaag cggcgcagga atttatcgca cagcaccacc gacacaataa accaccagtg
     2521 gggcataaat tcagcattgg tctgagaaat aatgccggag aattgatagg tgtggcgaca
     2581 gctggtcgac ctgttgcacg acatttggac gatggattaa cgcttgaagt aaatcgcaca
     2641 tgtaccacag gagaacgcaa cgctaacagc gcgctttatg gtgctgtctg gcgggcagca
     2701 aaagctatgg gttatcaacg ttgtattacg tacacccagg cagatgaatc aggagcatct
     2761 cttcgcgcag ctggttttgt tcgtgtgaaa gagcttcctc caagaaaaag ctgggcggaa
     2821 tcaagcgtcg ccctgcggag taaacgcgat ccggtcggaa acggtggtat tcctcgtgtg
     2881 ctctgggaaa tcaggagaat gagtaccgct ggcattcgca tcaaaggaga gtgatatgag
     2941 cgctataacc aaagaacgta tcaaattatt cattaaaaat ccgcttgata acggacttac
     3001 tcgtggcgaa caaatggaac tggcacgaat tgcactggca tcactggaac gcgaacagat
     3061 tcgccacgag catgccaaat ggtctgactc cacatttggc tgcgttggcc ccattggtcc
     3121 gctgaaacat ctctcaaaag aggcactgga agccgcagcc gaaccagacg atcttggcga
     3181 gtgggctgat atgcagtttc tgttgtggga tgcacagcgc cgtgctggca tcagcgatgc
     3241 tgaaattacc gctgctatgg aagataaatt gaagatcaac atgaagcgcc agtggcctga
     3301 accaaaagat ggtgagcctc gcttgcacat taaagaaccc ggcaactctc cggtaactcc
     3361 ggatggttgg ataagctgta gtgatcgaat gcctgaaaag ggccagaacg tgcttatttc
     3421 ggtgaatttc gatagctctc tggttgaacc gctaatatgc tctgcacgct ataccggaag
     3481 cacattccgg cgaggagatg caacgattaa gccgggtaat ggtattgagc aggcaactca
     3541 ctggatgaaa ttaccggaac cgccgcagga ggtgagctga tggaaaatga aggcgataat
     3601 atcatcacac tggtgcagcc aaagcgcgat gaagagaagc tgctgaacat tacagtaact
     3661 ggcagaaaaa actacacaca acagagctgt aagcatcggg ccattgaggt tcatgaacag
     3721 gatcgcgtta tcctgtgctt gcagtgcgga tgtgttgtgg acccttttca gtatgttctg
     3781 cggtgtgcga acgatggtga ggctgtggtg agagagatta gacagcttca taacagacgc
     3841 gatcagctcc gcgaatctgt tgccagcctc gagcgtgaag aaaagaacac caaagcacgg
     3901 ttgcgggcag caagaactgc aatactgtat gcggaaaatg accttaaaaa tattgagcag
     3961 aaggtgaatc aatgacctgg cctgaagcat tcacaacggt aggaatcgcg gtggcgctgg
     4021 tggtgtattc gatttgccgc tggggataaa aacggtttgc gggaaaagga gagttaagta
     4081 gaattgcagc gggtgcttga ggctatctgc ctcaggcatg atcaccaaaa ggcagataga
     4141 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggccata
     4201 tctatgcgac acatagagat tagcctctta cggaccgaaa ggtcaaggag aagcaggcta
     4261 tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga
     4321 cggcact
//
LOCUS       DAZUGK010000282         4305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZUGK010000282 DAZUGK010000000
VERSION     DAZUGK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.284"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..290)
                     /locus_tag="ACHY5W_004443"
     CDS             complement(<1..290)
                     /locus_tag="ACHY5W_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4449194.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAA"
     gene            complement(405..480)
                     /locus_tag="ACHY5W_004444"
     tRNA            complement(405..480)
                     /locus_tag="ACHY5W_004444"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Thr,seq:ggt)
     gene            complement(487..561)
                     /locus_tag="ACHY5W_004445"
     tRNA            complement(487..561)
                     /locus_tag="ACHY5W_004445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:Gly,seq:tcc)
     gene            complement(678..762)
                     /locus_tag="ACHY5W_004446"
     tRNA            complement(678..762)
                     /locus_tag="ACHY5W_004446"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(726..728),aa:Tyr,seq:gta)
     gene            complement(771..846)
                     /locus_tag="ACHY5W_004447"
     tRNA            complement(771..846)
                     /locus_tag="ACHY5W_004447"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(811..813),aa:Thr,seq:tgt)
     gene            1208..2158
                     /gene="coaA"
                     /locus_tag="ACHY5W_004448"
     CDS             1208..2158
                     /gene="coaA"
                     /locus_tag="ACHY5W_004448"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4449195.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2187..3152)
                     /gene="birA"
                     /locus_tag="ACHY5W_004449"
     CDS             complement(2187..3152)
                     /gene="birA"
                     /locus_tag="ACHY5W_004449"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFS4449196.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGVTLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3149..4177)
                     /gene="murB"
                     /locus_tag="ACHY5W_004450"
     CDS             complement(3149..4177)
                     /gene="murB"
                     /locus_tag="ACHY5W_004450"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4449197.1"
                     /translation="MNHSLKSWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHYEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQTDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1107 a         1065 c          972 g         1161 t
ORIGIN      
        1 gcagcaccag tgatcatgtt tttaacatag tcggcgtgcc ccgggcagtc tacgtgtgcg
       61 tagtgacggg tcggggtgtc gtattcaacg tgagaagtgt tgatggtgat accacgagct
      121 ttttcttccg gcgcgttatc gatctggtcg aatgcgcgag cagcaccgcc gtaggtttta
      181 gccagtacgg tagtgattgc agcggtcagc gttgttttac catggtcaac gtggccgata
      241 gtaccgacgt taacgtgcgg ttttgtacgt tcaaactttt ctttagacat cgattgtccc
      301 tctaagacac ggataaatcg gtgatatcac cacatcaacc aggcaacatg cccgacttgt
      361 tgaatgcaat aaacagaagg aaaaaacagg gaggagaaaa gaagtggtgc tgataggcag
      421 attcgaactg ccgacctcac ccttaccaag ggtgcgctct accaactgag ctatatcagc
      481 acatcttgga gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa
      541 tagccattat acgatgcccg catcctggaa ctcggctacc tgattttcat tctgcactga
      601 atatcgagag aagctctctt tattcgagcc ggtaagcgaa cttatcgtct cgggctacgc
      661 catcgcgtgg ccgaaattgg tggtggggga aggattcgaa ccttcgaagt ctgtgacggc
      721 agatttacag tctgctccct ttggccgctc gggaacccca ccggacttga tggtgccgac
      781 taccggaatc gaactggtga cctactgatt acaagtcagt tgctctacct actgagctaa
      841 gtcggcatca agtagcgcgc attctatgga gacatgcgag ttcatgcaac taaaaaattg
      901 cataatttgt tttattggtc acattttatg cgacacgatg aagaaacagc cgaatggagg
      961 gcagattagc atactttttc aacatcattg tgctcaacaa tgcgctcctg ctaaaccata
     1021 attcttttta tcagatggaa tatctgtcac attgcttttc aacgatagct tcctgggaga
     1081 gattttttct tattattcct ccccatctgg tgttaccctc ctgcccatta acccattcaa
     1141 cagaactgtg acgcgccatg gcaaatatcg ctttgccgat agagctatga ccgccagaaa
     1201 catgcttatg agtataaaag agcaaacgtt aatgacgcct tacctacagt ttgaccgcaa
     1261 ccagtgggca gctctgcgtg attccgtacc tatgacgtta tcggaagatg agatcgcccg
     1321 tctcaaaggt attaatgaag atctctcgtt agaagaagtt gccgagatct atttacctct
     1381 gtcacgtttg ctgaacttct atataagctc gaatctgcgc cgtcaggcag ttctggaaca
     1441 gtttcttggt actaacgggc aacgcattcc ttacattatc agtattgctg gtagtgtcgc
     1501 ggtggggaaa agtacaaccg cccgtgtatt gcaggcacta ttaagccgtt ggccggaaca
     1561 tcgtcgtgtt gaactgatca ctacagatgg cttccttcac cctaatcagg ttctgaaaga
     1621 acgtggtctg atgaagaaga aaggcttccc ggaatcgtat gatatgcatc gcctggtgaa
     1681 gtttgtttcc gatctcaaat ccggcgtgcc aaacgttaca gcccctgttt actcgcatct
     1741 tatttatgat gtgatcccgg atggagataa aactgttgtt cagcctgata ttttaattct
     1801 tgaagggtta aatgtcttac agagcgggat ggattatcca cacgatccac atcatgtatt
     1861 tgtttctgat tttgtcgatt tttcgatata tgttgatgct ccggaagact tacttcagac
     1921 gtggtatatc aaccgttttc tgaaattccg cgaaggggct tttacagacc cggattccta
     1981 ttttcataac tacgcgaaat taactaaaga agaagctatt aagactgcca tgacattgtg
     2041 gaaagagatc aactggctga acttaaagca aaatattcta cctactcgag agcgtgccag
     2101 tttgatcctg acgaaaagtg ctaatcatgc ggtagaagag gtcagactac gcaaataatt
     2161 tgcaggggag ctaatgctcc cctttcttat ttttctgcac tacgcaggga tatttcaccg
     2221 cccatccagg gttttattat tccatcctgc tcaagcaata aagcgccctg tttgtctatt
     2281 ccgcgtgaaa taccaaatat ttctttatca ccaatgataa gtttcactgg gcgattaata
     2341 aaattatcca gcttttccca gcgcgaaaga taaggtgcca atccttcttg ttcgaagagt
     2401 tccagcgccg cgcgtaattc acgtattagc atggccgcca acgtattacg atcgagattg
     2461 atccccgctt cctgcagcgt gatccacccc tgattaacga cactctcttc aacacgacgc
     2521 attgccatgt tgatcccggc tccaatgact atttgcgccg catcgccggt tttaccagtc
     2581 agctccacaa gaatgcctgc cagcttgcga tcctgcagat agaggtcatt aggccatttg
     2641 acacgaactt tatctgctcc cagcttgcgt aatacttccg ccatcacgat accgataacc
     2701 agacttaaac caatcgccgc cgccgggcct tgttccagac gccagaacat agacaaatat
     2761 aagtttgcgc caaaaggcga aaaccatttc cgaccccggc gaccacggcc agcctgctgg
     2821 tattctgcaa tgcaagcatc gcccgattta agctctccga tacgatcaag aaggtactga
     2881 ttcgtggagt caatcactgg cagcacggct acactaccgc catccagctg acccaatatc
     2941 tgttcagcat taagtaactg gataggctca ggcaggctgt atcctttacc cggaacggta
     3001 aagacatcaa cgccccagtc acgcagtgtc tgaatgtgtt tattaatagc cgcccggctc
     3061 attcccagcg ttacacccaa ctgctcgcca gagtgaaatt caccgttcgc taacagggca
     3121 atcaatttca gtggcacggt gttatccttc atgaaattgt ctccactgcg ctcacttcac
     3181 ctgatgcacc aataaagcgg acttcaggct caagccagac attaaatttt tcaccaactt
     3241 tctgtcttac atgatgcgcc agctgtacaa catcttcgct ttttgcatcg tgctcattta
     3301 tgagaaccag cgcctgttga cggtgtaccg cagccccacc aatttgcgtc cctttaagct
     3361 ggcactgatc aataagccaa cctgcagcca gttttactga accatctgtc tgggggtaat
     3421 gtggggctgt tggaaattgt gccaacaacg cgttagcagt ttcggcagaa acaacaggat
     3481 ttttgaagaa actacccgca ttaccgttca cttttggatc gggaagtttt gtagtcctca
     3541 tatgacatac cgcatcaaat acttgctgtg gcgttactgt tgtaggatcc agacgagtta
     3601 agtcaccata cgttagtaca ggttgccact cttttggcag acgcagacct acggctacaa
     3661 tggcgaagcg gtcctggtat tcatgtttaa aaatactgtc gcgatagcca aaacggcact
     3721 ctttggcagt taagcgcact tgcttgcctg tcgccagttc aacacaatca acataagcgc
     3781 aaactcgctg taattctacg ccataagcac caatattctg gataggtgat gagccgacac
     3841 aaccaggaat taatgccaga ttttccagac caggcatacc ttcctgcaaa gtgtatttta
     3901 ccaggcgatg ccagttttct ccggctccta catgtaaata ccacgcatca ggttcataat
     3961 gaatttcgat acctttgatc cggttgatga tcaccgtgcc gcgatagtct tccagaaaaa
     4021 gtacattact tccttctccc agaataagaa cgggttgccc ttctgcggtt gcatgctgcc
     4081 aggcattgag tagttgttgt tcgtcttcgg cacatacaat gtgctgagca ttatgatcaa
     4141 tgccaaatgt gttccaggat tttaaggagt ggttcatagc tgctttcctg atgcaaaaac
     4201 gaggctagtt taccgtatct gtggggggat ggcttgtagt tatgacgaca gggagagttt
     4261 gtagaaacgc aaaaaggccc agtcttccga ccgggccttt cgctt
//
LOCUS       DAZUGK010000283         4304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZUGK010000283 DAZUGK010000000
VERSION     DAZUGK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.285"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..502)
                     /gene="terC"
                     /locus_tag="ACHY5W_004451"
     CDS             complement(<1..502)
                     /gene="terC"
                     /locus_tag="ACHY5W_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFS4449198.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQH"
     gene            complement(525..980)
                     /locus_tag="ACHY5W_004452"
     CDS             complement(525..980)
                     /locus_tag="ACHY5W_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS4449199.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(1003..2160)
                     /locus_tag="ACHY5W_004453"
     CDS             complement(1003..2160)
                     /locus_tag="ACHY5W_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4449200.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(2160..2741)
                     /locus_tag="ACHY5W_004454"
     CDS             complement(2160..2741)
                     /locus_tag="ACHY5W_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4449201.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            3064..4122
                     /locus_tag="ACHY5W_004455"
     CDS             3064..4122
                     /locus_tag="ACHY5W_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS4449202.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            4132..>4304
                     /locus_tag="ACHY5W_004456"
     CDS             4132..>4304
                     /locus_tag="ACHY5W_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFS4449203.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIP"
BASE COUNT         1094 a         1124 c         1030 g         1056 t
ORIGIN      
        1 gatgctggga gtaatcctca atttcatcat cgtcatcacc gcttttaagc atcatgaccg
       61 ccgtccaggc aacgataata gcgaagacaa cttcaacgta cggccccaga ctcagcaggc
      121 tcgtaccgat ggcgacaaag atgcccctga agacgatggc accaatgatc ccccagtaga
      181 gaacacggtg gcgataacga tccggaacgg cgaaccagga gaagattgcc atcatgacga
      241 acaggttatc gaccgacagc actttctcca gcgcataacc cgtgacaaac agactggcaa
      301 cctcagcacc gtggtggata tagaggaaac cggcaaatgc catcgcaacc acaacccaga
      361 atacggacca gagcgcagca ctcttcagcg agattggctt gtcatcacgg tgcataaaca
      421 ggtcaataaa gatggcaccg actgaaagcg caataaaaac aatgaccgtt tcagtcggga
      481 agccgatgtg tgtggaaacc atattttacc cttagggatg tggatcagag gccaaattca
      541 gccgggtcga agcccaacgc gatagcgatc tcgcgggagg ccagtttctc gtcctgatcg
      601 aagttaccgt cacttttcgc aacggcaata ccaacacgta aggccagctg agcggcctca
      661 ggctgatctt tcagcgccag gatgtatttc atggtttcgc ccttgccaat ttcaacatcg
      721 aaatcgaagc ttgaaaccag tttattgaag aactcaatca cctcattggt atcgaagacc
      781 ttcagctctt ctgaagagcg cagaaagccc atcattttct gcttttcttc cgaacttacg
      841 ccgtcactcg atacggcaat acgggcacat acagcaacgg tgccctgcat gaattttttg
      901 tttttgaaac ggccaacctg gcgggtcagt tcgtcacggc ctgagttaat ggcaccttta
      961 actttgtcga aaaagctcat acctgtttcc tttgttgctg tgttatttag aaccggcgct
     1021 ccagcgaaat ccccagccaa atgcccggtc catttcgtcc tggcctttga agtactggtt
     1081 aattcgctcg actttaattg cgccgttttc gtttacgagt ctggcaatgg cgcacaatgt
     1141 gcgacggttc tcaccttcgg tcaggcgggt ctcaatgggc ggttgatccg gcacatgaat
     1201 ggtgactaca ccatcagtct tgtcccagct gggaacacct tcataaataa aggcgtaaat
     1261 cagcacttcg cggatatgct tccattcacg tccattgata tgcagccact cgccgtctga
     1321 tacatcaccc gtccggtcat cgcccttgag ctgaacatac ggttcatcgc gataatcacc
     1381 gaatgcatta ccgagcgcct gaatgaccga tttatacccg tcctgaagct caacaaaggc
     1441 ccccagatcc aggtcgattc ctttggagcc gaacatacct gcaaagcctg atttgctact
     1501 gccccggtgc cagttcaggt tgatccggat ttcaccgaag ttatcccgtt tggtcaggct
     1561 gattgcaggc ttctcttttg tcagcgatac cttgctcagg ctgatttttg tctcaactgg
     1621 cggaggggtt acaaccggag gagttggggc tgcgggagcc ggttcgtcgg caatatttac
     1681 gccgaaatgc tctgccagcg gtttaagtcc accgttgaac ccctgcgcaa cgaagcggaa
     1741 tttccagtca ttattacgac ggtaaaattc ccccagaatt aaggctgctt cctgacggcc
     1801 gctcagttcg acactgccgc taaccaggcc ggtagcccct tgctccacgt cgatggaaag
     1861 attacgaaga ccggacaccg tctgcccacc gtcacaggtc acggtgaacg caattttctg
     1921 gacatcaggt ttcagccggt tgagcgcgac agtaaaggtt gagtactggc cttcactgac
     1981 caggctgacg gtaccatcat cgttacgtgg ctgaccataa aacaccatgt ccgcatcacc
     2041 ctgcaccttc ccgtcggcat acaggcgaaa cgccgaagca tccacctggc cgcccgaggt
     2101 gatccggacc cgcaactcct gggctggaac gggggcattg ccccccggcg tcaggttcat
     2161 tagcgaataa ccgctgaaac gatatcggag tgcatatcgt caattgtgcg gccgcgacag
     2221 gcatggccaa gggcggtaaa gtcccagttg ccgttattac ggcgcagcga agaaataacg
     2281 atcccggtat gtgagccctg ctcagtcagc ttgtagcgag ccagctcttt gccggtttga
     2341 tcgacaacgc ggcagaaagc gttttcgacg tcattgaacg actgaccacg gaagctgttt
     2401 accgtaaacg ccaggtattc aacgtttgca ggcaggcggg agaggttgac cttaatcact
     2461 tcatcgtcac cgtcaccctc accggtcagg ttatcgccac tgtgcacaac agcgccgcag
     2521 gtggatttca gtttgcggaa ccagatggtg tcaattgttt tacctgtgct gtccattaat
     2581 acacagccag catcgagatc gattgagtcg ttgcctccaa acaatcctcc aagaagacct
     2641 tttttcttaa ccggatccca accgagaccg aaatgaagct ggcttaatgc agatgattct
     2701 ttgctgagtg ataccgtctg gttcttaact aatgaaacca tgttttattc cttaaaatgg
     2761 taatgaccga gacaatcaaa ggtatattta gtaactgacg aagaaaatca tatcatgatg
     2821 aagttctctg acaagggaaa tttttgtggt tttctgtaaa aacacaaaaa gaaatccttt
     2881 tcagggtgtt agtttttcta tgtttaattt aaatatcaat gtgttataag gaatccaaaa
     2941 aacactgacg cactccataa ccacaggagt tattaatcat attatgattg acatgtagct
     3001 tagacactca tagactcact tctcaatcat aaatttgcat ggcgttccgg ataattaaaa
     3061 aaaatgaaca agaaaatttg gtttatggaa ggtttatcct cccagcgaga tattattcag
     3121 ggagtaaaat catttgcaca aaaaaataat tttgccatta ccgtttttgc ctcccaccgt
     3181 aacgaaagaa atgaaatcct ttccgttgcc gattattctt tgactgaacc tgaagatcct
     3241 caaaaacgtc ttcagtttat ccaggaaacc attcagacct acggcatcca ccatattcat
     3301 actggccgta acagccagtg gtttgaagaa caccgttcag ccattgaacc gaccggtgcc
     3361 acccttacta ccggtgcaac gggcgtcgac tggttaactc tggctgacga aaaagttact
     3421 tttgctcagt ttatggagca aaagggtctc ccggtcgtac catcctggcg ggtgaatacg
     3481 ctggcagaat taaagacaca cctcgcggcc ccgccgttca ctgacagccc ggtatgcgtg
     3541 aagccggtga cgggtatcta tggcatggga ttctggcgct ttgatgacag tgcttcgcct
     3601 atggccgtct ttaatcatcc cgaacatcgt ctggtcagtc cgcaacagta tattgcagca
     3661 gcatcagctg ctgagtcgtt taaacccctt gttttgatgc cgtacctgcc aggcccggaa
     3721 ttttccgtcg atatcctcgc ggataagggc gaaatactcg cagccgtggg acgccgtaag
     3781 gaaggggcta tccagtatct ggtaaacgaa ggaagcgcct gggaactggc gtgtgactgc
     3841 gcccgtgtta tgaaggccga cgggctggtg aatgttcaga cgcgaaacga tgtgaatggc
     3901 aacccggtgc tgcttgaaac caacatgcgt ccgtcagggg gggtgggtta tacccttcac
     3961 agcggtgtga accttcctgg gttatttgct gcctttaagc tcggtctgat gtctgaagat
     4021 atggtacgcc agagcgctaa aaacaccttt tctccggttg cggtgagatc cattacggat
     4081 gtaattgcat acccggaatc actctctaac cttctgaatt aaggcgtatt tatgtctgat
     4141 atcctcgaag atatgattta tcgccgtacc ctctcatgcg gtacgattca ggtaacccgt
     4201 gaccagggtg aagtttcgct cgatgacctg tttgacatcg ctgaaagacg taacccgaaa
     4261 cgcgcctttc tgtttgtcag caaagtactc ggcaggcaca ttcc
//
LOCUS       DAZUGK010000284         4298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZUGK010000284 DAZUGK010000000
VERSION     DAZUGK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.286"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2277
                     /locus_tag="ACHY5W_004457"
     CDS             <1..2277
                     /locus_tag="ACHY5W_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFS4449204.1"
                     /translation="LARSMDVDIYNLHPAHAKRVEEIISENKPPFSVFRDKFITMPGG
                     LDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLIETDHANPDPEIVDIACGRSSAPM
                     PQRVTEEGKHDGEEKPQPSCAMADKQATAETVEPDATEHHQDTQPLDAQSQVNSVDAK
                     YQKLRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQ
                     NQPETKQNTPETVKTSPDVKQPEPVVQQKPEIVCNACGQTGGDNCPDCGAVMGDATYQ
                     ETFDEENQVEAKENDPEEMEGAEHPHNENAGSDPHRDCSDETGEASAPVATEIMWPSY
                     FEPGRYENLPNEVYHSANGISSTMLKDARISLMYYHGRHIAGTIPNEESDALLRGRII
                     HSYVLETDKFADEYAIPVPVPEYVVTTSSELIAIIKKHNASLPALMTPEQMKEWIESY
                     NSTLIQPLSVSAGAEETGILYGSLPVEFRRIPEGEKHTASAMKACIKEYNASLPPLLK
                     TSGAREQLLDQIETVDPELAKKERAKSLPYNISGTKEQLTEIARKIRPELVTLEDWQK
                     RQQEENAGKTFISPEMYEQAKNIHAALQNNTDAARLLNHPDRKSEISYFGFDEETGLE
                     IRVRPDIEIRLPYESICADVKSVSLGYVRQERLKDRLHREIIERDYHLSAAMYCDVAN
                     LDKFFWIFVNKDAGYHWVAVVEASQELLELGRQEYRRTLRQINEALETNNWPAPITES
                     YTDELNDFDLRRLEALSI"
     gene            2289..3341
                     /locus_tag="ACHY5W_004458"
     CDS             2289..3341
                     /locus_tag="ACHY5W_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecT family recombinase"
                     /protein_id="HFS4449205.1"
                     /translation="MNELTQQENINSNVAVFSPQSMAAIQTFSQVMASGMATVPEHLR
                     GNPSDCMAITMQAMQWQMNPYAVAQKTFVVNGVLGYEAQLVNAVISTRGPLTGRIEYD
                     WFGPWEKIIGKFEIRKSDKGKEYRVPGWKLADENGIGVRVQATLRGESKPRVLELLLA
                     QARTRNSTLWADDPRQQLAYLALKRWARLYCPEVILGVYTRDELDEPQEKIINPVQEH
                     KNTSACRAERETTIIEQDAGENWIDAFRERIEQAQSTGETTALRQEVEDHKNTLGALY
                     TELKGKVVQRHHRLNAIARIEKMINDLPSSGDPEAEQKFTALENTLNAARPHLGELYE
                     AYKTTLTDMKPEYIGS"
     gene            3405..3599
                     /gene="ralR"
                     /locus_tag="ACHY5W_004459"
     CDS             3405..3599
                     /gene="ralR"
                     /locus_tag="ACHY5W_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFS4449206.1"
                     /translation="MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            3592..3780
                     /locus_tag="ACHY5W_004460"
     CDS             3592..3780
                     /locus_tag="ACHY5W_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS4449207.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKTKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            3887..4168
                     /locus_tag="ACHY5W_004461"
     CDS             3887..4168
                     /locus_tag="ACHY5W_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4449208.1"
                     /translation="MNLVTLKTWGRLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            4134..>4298
                     /locus_tag="ACHY5W_004462"
     CDS             4134..>4298
                     /locus_tag="ACHY5W_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4449209.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRNA
                     VAQAIEANHYI"
BASE COUNT         1357 a          989 c         1042 g          910 t
ORIGIN      
        1 ctggcccgtt caatggacgt ggacatttat aaccttcatc cggcacacgc taaacgtgtc
       61 gaggaaatta tctctgaaaa taaaccgccc ttttctgttt tccgcgacaa attcatcacc
      121 atgcctggcg ggctggatta ttcccgcgcc atcgtggttg cgtccgtaaa agaagcacca
      181 attgggatcg aggttatccc tgcgcatgtc actgaatatc tgaacaaggt gctgattgaa
      241 accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg ttcctctgcc
      301 ccaatgccgc agcgtgtaac agaagaagga aaacacgatg gtgaagaaaa accgcaacca
      361 tcttgcgcaa tggcagataa acaggcaacg gctgaaacag tggaaccgga tgcaactgaa
      421 catcatcagg acacgcagcc gctggatgct cagtcacagg taaattctgt tgatgcgaaa
      481 tatcagaaac tgcgggcaga actccatgaa gcccggaaaa acattccatc aaaaaatcct
      541 gtcgatgccg ataaattgct tgctgcatca cgtggtgaat ttgttgacgg aattagcgac
      601 ccgaacgatc cgaaatgggt aaaggggatc cagactcgcg actctgtgta ccagaaccag
      661 ccagaaacga aacaaaacac gccagaaact gtaaaaacca gcccggatgt gaaacaacct
      721 gagccagtag tgcaacagaa accggaaata gtctgcaatg cctgcggtca gactggcggg
      781 gataactgcc ctgactgtgg tgcggtgatg ggcgacgcaa cataccagga aacattcgat
      841 gaagagaatc aggttgaagc taaggaaaat gatccggagg aaatggaagg cgctgaacat
      901 ccgcacaatg agaatgctgg cagcgatccg catcgtgatt gcagtgatga aactggtgaa
      961 gcgtcagctc ctgtagcaac tgaaatcatg tggccgtcat atttcgagcc tggccgctat
     1021 gaaaacctcc cgaacgaggt ttatcactcc gccaacggaa taagcagcac gatgctgaag
     1081 gatgcccgta tcagcctgat gtattaccac ggacggcaca ttgccggaac tattccgaac
     1141 gaggaaagtg atgcactgct gcgtgggcgg atcattcaca gctatgttct ggaaacggat
     1201 aaattcgctg atgaatatgc cattccggta ccggttcctg aatatgtggt tactacttct
     1261 agcgaactga tcgccatcat taaaaaacac aatgccagtc tgccagcact gatgacacca
     1321 gagcagatga aagagtggat cgaaagctac aacagcactc ttatacagcc actgtctgta
     1381 agtgccgggg ccgaagaaac aggcatcctt tacggttcgc ttccggtgga atttcggcgt
     1441 attcctgagg gggaaaaaca cacagcatca gcaatgaaag cctgtattaa agaatacaac
     1501 gcaagcctcc ctcctctgtt gaaaaccagt ggagcacggg agcagcttct ggatcaaatt
     1561 gaaactgtag acccagaact ggcaaaaaaa gaacgtgcta aatctttgcc ttacaacatc
     1621 agtggcacaa aagagcaatt aaccgaaatc gcacggaaaa ttcgcccgga actggtgaca
     1681 ctggaggact ggcaaaaacg ccagcaagaa gaaaatgccg ggaaaacgtt tatcagtccg
     1741 gagatgtatg aacaggcaaa aaatattcac gcggcactgc aaaacaatac cgatgcagca
     1801 aggctactca accacccgga tcgcaaatct gaaatcagct atttcgggtt tgatgaagaa
     1861 accgggctgg aaatcagggt ccgtcctgat atcgaaatcc ggctgccata cgaaagcatt
     1921 tgcgctgacg tgaagtcagt cagcctcggt tatgtgcgac aggaacgact taaagatcgc
     1981 ctgcaccgtg aaattattga gcgtgattat cacctcagcg ccgcaatgta ttgcgatgtg
     2041 gcaaacctgg acaaattttt ctggatcttc gtcaacaaag atgctggcta tcactgggtg
     2101 gcagtcgtgg aagcctcgca ggaactcctg gaacttggtc gacaggaata tcgccggacg
     2161 ctacgccaga taaacgaagc cctggagaca aacaactggc cagcaccgat taccgaaagt
     2221 tataccgacg aattaaacga ctttgatctt cgtcgtcttg aagcactgag catctgagga
     2281 aggacacaat gaacgaatta actcaacaag aaaatattaa ctctaatgtt gcggttttca
     2341 gccctcagtc catggctgca attcagacat tttcccaggt aatggcttcc ggcatggcta
     2401 ctgtaccgga acacctccga ggaaatccat cagactgcat ggccatcacc atgcaggcga
     2461 tgcagtggca aatgaaccct tacgcagtag ctcagaaaac tttcgttgtg aatggtgtgc
     2521 tcggatatga agcgcaactg gttaatgccg taatcagtac tcgtgggccg ctaaccgggc
     2581 gtattgaata tgactggttc gggccgtggg aaaaaattat cgggaaattt gaaatcagga
     2641 agagcgacaa agggaaagaa tatcgtgtac ctggctggaa gctggccgat gaaaacggga
     2701 tcggtgttcg tgtccaggca acactacgcg gcgaaagtaa accacgcgta ctggaattac
     2761 ttctggcgca ggccagaaca cgtaactcaa cgctatgggc cgacgatcct cgccagcagc
     2821 ttgcctatct agcgctgaaa cgctgggcgc gcctttattg ccccgaagtg attcttggag
     2881 tgtacaccag ggacgaactg gacgagccac aggaaaaaat cattaatccg gttcaggaac
     2941 ataaaaacac ttccgcttgc cgcgcggaac gtgaaacaac aattattgag caggatgccg
     3001 gggaaaactg gatcgatgct ttccgtgaac gtattgagca ggcacaaagc accggggaaa
     3061 caacagcact tcgccaggaa gtggaagatc ataaaaatac acttggcgct ctctatacgg
     3121 aacttaaagg aaaagtggtt cagcgtcatc accgtctcaa tgctattgcc cgtatcgaga
     3181 agatgataaa tgacctacct tcttcaggtg atccagaagc agaacaaaaa tttactgctc
     3241 tggaaaatac gctgaatgct gcccggccac atctgggtga attatatgag gcgtataaaa
     3301 cgacactgac agatatgaaa ccagaatata tcggctcctg atattgactt tggcggtgta
     3361 gcctcaccgc catcacaaaa ttttatttta tgagagaaaa gacaatgcgg tatgaaaaag
     3421 tcaaaccatg ccctttttgt ggttgtccat cagtaacggt gaaagccatt tcaggatatt
     3481 accgcgcgaa gtgtaacgga tgcgaatccc gaaccggcta tggtggaagt gaaaaagaag
     3541 cactcgaacg atggaataaa cgaaccactg gaaataataa tggaggtgtt catgtataaa
     3601 attaccgcca ctattgaaaa ggaaggtggc actcctacta actggacaag gtactcaaaa
     3661 acaaaattaa ccaaatcaga atgcgaaaaa atgctctcag gtaaaaaaga agcaggcgtt
     3721 tccagagagc agaaagtaaa actgataaat tttaattgcg agaaacttct gtcctcgtga
     3781 gttgcattat atccaaatta gaacttcata gctgattatt aaaaatcaac cacacccgcc
     3841 agtattctgt atatttactg gcggtcatat cgtaagaggt atggcaatga atcttgtgac
     3901 actcaaaact tggggaagac tcagatatcc ggataaccca ccatcaatat caacgctgag
     3961 acgatgggca agaaatggaa acatttatcc tgcacctgaa ctacacggga ggagttacag
     4021 ggtggttccg gaggctttct atatcaaccc aaataaggtt gataccgata taacacacca
     4081 tcagcctaat gggcgacaag ggagagacag tccgttactg gagaagttaa aacatgcagc
     4141 ggaaaaaata cgatcccaat ttgcctagaa acttaacata tcgaaggagg gacaaagcat
     4201 attactggcg caaccctctg acgaaagaag aatttacact aggtaaaatt tcaagaagaa
     4261 atgcagtagc gcaggcaatt gaagcaaatc attatata
//
LOCUS       DAZUGK010000285         4244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZUGK010000285 DAZUGK010000000
VERSION     DAZUGK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.287"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..196
                     /locus_tag="ACHY5W_004463"
     CDS             <1..196
                     /locus_tag="ACHY5W_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFS4449210.1"
                     /translation="CFACFAKDERGVTAIEYALIGVAMATLLAFIFGDQNSGFLGAIK
                     DAFDAIAAAIQQVTVSGTNP"
     gene            261..728
                     /locus_tag="ACHY5W_004464"
     CDS             261..728
                     /locus_tag="ACHY5W_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFS4449211.1"
                     /translation="MHGIWSLSMAAIWLLRVDLLLVALFLVFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGTVLLVGFFLFSAGVIGGGDVKLLTILSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSISENGVPYAVPISLAFLLTYPVFPLFC"
     gene            747..1568
                     /locus_tag="ACHY5W_004465"
     CDS             747..1568
                     /locus_tag="ACHY5W_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020303802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaB"
                     /protein_id="HFS4449212.1"
                     /translation="MNHRMLFFLSVIVITVGLAGIFMQKSETIPSSIAPTKTPTKTIV
                     VAEATRELKPGHILVPGDYKISTFQINKDEKDIRDISSLGTRNLNGYLMRNNISQFSS
                     ILPEAVESPSSETFLVHSLGPDELPYGYPVRPEDEYLLTSLSAGDKVSLYIRLTEVEK
                     DKETKVGFVPEGSRQADKEMKKYVLSKLSEPLSILQIDKDKKVKSSGRSYGQAQPVGT
                     IVLRMNRKQLADLRVVEEVGDILLFPAANGQGNVKVRMDDVLPQFRSVKELRGHK"
     gene            1565..2956
                     /locus_tag="ACHY5W_004466"
     CDS             1565..2956
                     /locus_tag="ACHY5W_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004937775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HFS4449213.1"
                     /translation="MIKHSEPYFLSLFNRYKIAASLFLGVIGLCYSIGVLAQEIYMGS
                     GDSQVIQAQDDIDTVFISTPEIADYEIINDHSVMVYAREPGRTDLTVFDKNGDELVKT
                     TIIVDPVLSAVQKQISMISPDSHVSIQKMGKTYIISGTVPTEEDRDKVYQLVGEGIGA
                     KKVVNKKQVASLGASSGSDLNTDNSNSWLDEVIYEDVINKLQLPITNQVNVKLSVVEV
                     TKTFTDNVGIDWGTTGTSPGSFRFIKFDADTLTGLVHAISNDSVARVLAEPNLSVLSG
                     ETAEFLVGGEVPVVTSSGNNGTNVQYKEFGIKLNVGAKVSSTKNIRITLGEEVSNVDD
                     KFNTDSGDSFPTFQTRRARTTVELADGESFLLGGLISNNELESLSKIPFIGDVPILGS
                     LFRNASTERSRSELMVVATVNLVKPVGILDVVVPDFQRTSTWARFLNLDGISNSHDRK
                     QAQNFVEKGGFIK"
     gene            2953..3441
                     /locus_tag="ACHY5W_004467"
     CDS             2953..3441
                     /locus_tag="ACHY5W_004467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449214.1"
                     /translation="MIMLKKMLSLLLLGCLISQSFFCVAETYGLPELREQQFSLTDTG
                     QIAAEIITGDILSKVDKNISGSIKLSWSDEQYAPAAKHLRDMLIEGGIAPEKVLLDHA
                     SGGFSSTTNGIKILIQEIVMKLPECNYRAQGYRFDYHDEMGCAINNTRSSSIINPYKL
                     NY"
     gene            3454..>4244
                     /locus_tag="ACHY5W_004468"
     CDS             3454..>4244
                     /locus_tag="ACHY5W_004468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tight adherance operon protein"
                     /protein_id="HFS4449215.1"
                     /translation="MLLFPQKDDKSTVTEKIFFVCSAREKVCTTLSEMLRAAGFSHVK
                     CINQDLLKDSEVTFPENAAGVIIDVEKCDKSHNVTTIVQAVVPREVWCCVVGDSDSIS
                     LAQSFSRQGIFYFNAKVQTEELVTAATTGMTARSPRSTVCVSVLGCKGGIGNTEIAWQ
                     LAQKIVQRRSMSTLFVQGPSGSRDLDILVGKKILQDLVQVNKNLDAKRQDSESLPDFS
                     QNIYDKYNFVIFEETINSASKETLRHLAEKSSCLILVLDRSMSSVR"
BASE COUNT         1162 a          823 c          988 g         1271 t
ORIGIN      
        1 gtgctttgcg tgtttcgcga aggatgaacg tggtgttact gcaatagaat atgcattgat
       61 tggtgttgct atggcaactc tgcttgcttt catctttggt gatcagaact caggcttcct
      121 gggtgcaatt aaagatgcat tcgacgctat cgctgcagca attcagcaag taacggtttc
      181 cggaacgaat ccgtaaccta taaataataa aaaactctct agaggctgtc ggacataaag
      241 gagtatgtct ggcagcaacc ttgcatggta tatggagtct ttcaatggca gcaatatggt
      301 tactaagagt cgatctttta ttggttgcgc tctttcttgt ttttatttca tggggggata
      361 tattactcag aattattagt catcgttatt tactggtgtt aaccgtgctt atttttctgg
      421 cactcatttt acagcatcaa aaacccaatc tggttgctgc cgggaccgtt ttgctcgtgg
      481 gcttttttct tttctccgca ggggtaattg gcggcggtga tgtcaagtta ttaactattc
      541 tttcattagc tattgatgaa catgaactgg ccaattttct cgtcgcaatg accttttgcg
      601 gcgctctggt tgtccttgct ggtcttcttt tttttcgaaa atcaattagt gaaaacggtg
      661 tgccttatgc tgtaccaatt tcattggctt ttctgcttac atatcctgtt ttccctttgt
      721 tttgttaatt ctttaggaca gcataaatga atcatcgtat gctttttttt ctctcagtaa
      781 ttgtgataac agttggtctt gctggtattt ttatgcaaaa gagtgaaacc ataccatcct
      841 caattgctcc aacaaaaacg cctacaaaga ccatcgttgt tgctgaagcg actcgtgaac
      901 taaagccggg acatatttta gtacccggag attacaaaat aagcacattt cagataaata
      961 aagacgaaaa agacattaga gatatttctt cattgggtac aagaaatctc aatggttatc
     1021 ttatgcgcaa taatatttct caattctctt ctattttacc tgaagcagtt gagtccccga
     1081 gtagcgaaac cttccttgtt catagtctgg gacccgatga gcttccatat ggttacccgg
     1141 ttcggcctga ggacgagtac cttctcacct cattgtcagc cggcgataaa gtttctttat
     1201 atattcgtct tactgaggtt gaaaaagata aggaaactaa agtcggattt gttccggaag
     1261 gcagtagaca ggctgataaa gaaatgaaga aatatgtgct aagtaaactc tcagagccgc
     1321 ttagtatttt acagattgat aaagataaga aggtgaaatc gtcaggtcgt tcatatggtc
     1381 aggctcagcc ggtgggcact attgtattac gcatgaaccg gaaacaactt gctgacttac
     1441 gcgttgttga ggaggtgggc gatatcctgc tctttccggc agctaacggt caaggcaatg
     1501 tgaaagtaag aatggatgat gttctacctc aattccgttc agttaaagaa ttaagaggcc
     1561 acaagtgatt aagcattcag agccctattt tttgtcgtta tttaatcggt acaagattgc
     1621 agccagtctc tttttgggcg tgattggttt gtgctactcc ataggtgtat tagcacaaga
     1681 aatctatatg ggatcaggtg actcccaggt cattcaggcg caggatgata ttgatactgt
     1741 atttatttca acgcctgaga tagcagatta tgaaattata aacgaccaca gcgttatggt
     1801 ttacgcgcgc gaacctgggc gtaccgatct gactgtattt gataaaaatg gtgatgagct
     1861 ggttaaaaca acaattattg tcgatcctgt tttgagcgct gtacaaaaac aaatatcgat
     1921 gatttcgcct gacagccatg tttcaattca gaaaatgggc aaaacttata tcataagtgg
     1981 cacggtgcct accgaagagg acagggataa agtttatcag cttgttggtg aagggattgg
     2041 tgctaaaaaa gttgtgaata aaaaacaggt tgcctccctg ggcgcttcct cgggtagcga
     2101 tttaaataca gataactcta attcctggct ggatgaagtt atctatgaag acgtgattaa
     2161 taagcttcag ctacccatca ccaatcaggt aaacgtcaaa ctttccgttg tggaagtgac
     2221 taagacgttt actgacaacg tcgggatcga ttggggaaca acgggtactt ctcctggctc
     2281 gttccgcttt attaaatttg atgcggacac attaacggga ctggtccatg caatcagtaa
     2341 tgattcagtt gcacgtgtat tagctgaacc taacctttcc gttctgtccg gcgaaacagc
     2401 cgaatttctg gtcggggggg aagtccctgt ggttaccagt tcgggcaaca atggtactaa
     2461 cgtccagtat aaagagttcg gtatcaagtt gaatgttggg gccaaggtaa gcagcacaaa
     2521 gaatattcgt attactcttg gtgaagaagt cagtaatgta gatgacaaat ttaacactga
     2581 cagcggtgat agtttcccaa ctttccagac gcggcgggca cggacaaccg ttgaacttgc
     2641 tgatggcgaa agcttcttac tcggtggatt aatcagtaat aacgagcttg agtcattatc
     2701 taaaattcca ttcattggcg atgttcctat tctgggatca ctgttcagga atgcctctac
     2761 agaacgtagc cgtagcgaac ttatggttgt tgcaaccgta aatcttgtga aaccagtagg
     2821 cattcttgat gttgttgttc ctgatttcca aagaacatcg acatgggcac gttttctcaa
     2881 tctcgacgga atcagcaatt ctcacgatcg caaacaggcg cagaattttg tggagaaggg
     2941 agggtttatc aaatgatcat gcttaaaaaa atgctgagct tactattatt gggttgtctg
     3001 atatctcagt ccttcttctg cgttgcagaa acttatggat tgcccgaact tcgtgagcaa
     3061 caattttcac tgacagatac agggcaaatt gctgctgaaa ttatcactgg ggatatttta
     3121 tccaaagtag ataaaaacat ttctgggtct attaaattgt cctggagtga tgagcaatat
     3181 gcgcctgccg caaagcattt acgcgatatg ctaattgaag gaggcatcgc tccggagaaa
     3241 gttcttcttg atcatgccag tgggggattc tccagtacga ccaatggaat aaagatcctt
     3301 attcaggaaa ttgtcatgaa attacctgaa tgtaattatc gcgctcaggg atatcgtttt
     3361 gattatcacg acgagatggg ttgtgcaatt aacaatacac gtagttcatc cattattaat
     3421 ccctacaagt taaactatta agggctggca cagatgttat tattcccgca aaaagacgat
     3481 aaaagtactg ttacggagaa gatcttcttt gtctgttcgg caagagaaaa ggtctgtaca
     3541 acgttgagcg agatgcttcg ggcggctggc ttcagccatg ttaagtgcat taatcaggac
     3601 cttctaaaag acagtgaggt tacgttcccg gagaatgcgg caggcgttat tatcgatgtt
     3661 gaaaaatgtg acaagtctca taatgtaacg actattgttc aggctgttgt gcctcgtgaa
     3721 gtgtggtgct gtgtcgtagg agatagtgat tctatttcgc ttgcgcagtc tttctctcgt
     3781 cagggtattt tttactttaa tgccaaagtt cagactgaag agcttgttac tgcggcaacc
     3841 accggaatga ctgcgcgttc accgcgcagc acggtgtgtg taagtgtgtt gggctgtaag
     3901 ggggggatcg gtaacaccga gatagcctgg cagctggcgc aaaaaattgt tcagcgcaga
     3961 tcaatgtcca ctttatttgt acagggaccg tcgggatcac gcgatctgga cattctggtc
     4021 ggaaaaaaaa ttctccagga tttggtacag gtaaataaaa atcttgatgc caaacgacag
     4081 gatagcgaga gtctgcctga ctttagtcaa aacatttacg acaaatataa ctttgttatc
     4141 tttgaggaaa ctatcaattc tgccagtaaa gaaacgctgc ggcacctggc ggagaaatcc
     4201 tcttgtctga ttctggttct tgaccggtca atgtcatcgg tgcg
//
LOCUS       DAZUGK010000286         4194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZUGK010000286 DAZUGK010000000
VERSION     DAZUGK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.288"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHY5W_004469"
     CDS             complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHY5W_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4449216.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIVLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHY5W_004470"
     CDS             complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHY5W_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4449217.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHY5W_004471"
     CDS             complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHY5W_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4449218.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4193)
                     /locus_tag="ACHY5W_004472"
     CDS             complement(3192..4193)
                     /locus_tag="ACHY5W_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4449219.1"
                     /translation="MAAASVLLAVANQAHAGSTLDAVQKKGFVQCGISDGLPGFSYAD
                     ADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLSRNTTWTSS
                     RDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELNVADYFKAN
                     NMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVLPEVISKEP
                     LGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAHLLGKEGDY
                     GKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGIQYAPPVR"
BASE COUNT         1157 a         1114 c          988 g          935 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgacttag gatgcgcaaa
      121 aaattcatca ggtgcagctt gctccactat ttccccacga tccataaaaa ttacccggtc
      181 agcgacggtt cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagcaca atcatcgtat ccagcacctc tttcaccatc tcaggatcga gcgccgacgt
      301 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tttgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat caatttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa cagaacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca actgatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agagcaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg tttgagttat ggctcgccgg gcgcggggga tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc agactgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tgattcagca cggtgccggc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggtggaat aatcacccgc aatgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtcgcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggtgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat caacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagcggtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaatat caacatcaat acctgaaaat ttaccgtcag catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgtacggc atcaagcgtc
     4141 gagccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagt
//
LOCUS       DAZUGK010000287         4180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZUGK010000287 DAZUGK010000000
VERSION     DAZUGK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.289"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..944
                     /gene="ybeF"
                     /locus_tag="ACHY5W_004473"
     CDS             <1..944
                     /gene="ybeF"
                     /locus_tag="ACHY5W_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFS4449220.1"
                     /translation="NQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVN
                     AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGA
                     LDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQ
                     TDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQ
                     NFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKL
                     PFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1153..2118
                     /gene="lipA"
                     /locus_tag="ACHY5W_004474"
     CDS             1153..2118
                     /gene="lipA"
                     /locus_tag="ACHY5W_004474"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFS4449221.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHY5W_004475"
     CDS             complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHY5W_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFS4449222.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2551..3339)
                     /locus_tag="ACHY5W_004476"
     CDS             complement(2551..3339)
                     /locus_tag="ACHY5W_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFS4449223.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFASPQLL"
     gene            3432..3815
                     /gene="crcB"
                     /locus_tag="ACHY5W_004477"
     CDS             3432..3815
                     /gene="crcB"
                     /locus_tag="ACHY5W_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFS4449224.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHY5W_004478"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHY5W_004478"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS4449225.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1096 a         1075 c          951 g         1058 t
ORIGIN      
        1 ataatcaaat tgaatcctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta
       61 caaccttacg aaatattgat cttaatcttc tgactatttt tgaagctgta tatgtacata
      121 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
      181 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
      241 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
      301 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
      361 ccccctcggt cggagccctg gtccttcctg tcatttatcg ggcgattaaa actcactatc
      421 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
      481 aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
      541 tgttcaccga caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag
      601 atgacagaga gactatcgac aacgctgcac atgtactcct gttaccggaa ggacaaaatt
      661 tcagcggtct gcggcagaga attcaagaga tgtttccgga ccggcagatc agcttcacca
      721 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
      781 ccagccgttt ttataacctg tttagccgtt gctggccgct ggaaaaactg ccttttccgt
      841 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc
      901 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
      961 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
     1021 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
     1081 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
     1141 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgtaaaata ccgcgatgcc
     1201 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
     1261 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
     1321 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
     1381 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc
     1441 cgttgtccgt tctgtgacgt tgcccacggt cggccggtag ctcctgatgc caatgagcca
     1501 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
     1561 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
     1621 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgtatg
     1681 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
     1741 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
     1801 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
     1861 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
     1921 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
     1981 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
     2041 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
     2101 gggatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
     2161 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
     2221 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
     2281 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
     2341 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
     2401 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
     2461 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
     2521 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcgacg caaagcgacg
     2581 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
     2641 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
     2701 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
     2761 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccgcgaac
     2821 ccaggcggca ggaagcacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
     2881 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa
     2941 cggagctatt tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata
     3001 gagatgcagt ttggcataac gggcgacaat gtttcctgcc tgtagcgcca ccagcatatt
     3061 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
     3121 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     3181 cgaaagatcc gcatcatgat catcgcgtgc cagcaatgct tccggcagga caaacagcga
     3241 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     3301 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
     3361 taaaattcgc caacgaagat tattcggcgt tacacttatc actcatacaa atcaaatagc
     3421 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     3481 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     3541 cgctggctgc aaatctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     3601 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     3661 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     3721 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     3781 cattctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
     3841 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     3901 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     3961 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     4021 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     4081 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg
     4141 tggtacgctt tgtccagtgc aacttcacta aaaagtgata
//
LOCUS       DAZUGK010000288         4173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZUGK010000288 DAZUGK010000000
VERSION     DAZUGK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.290"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..491)
                     /locus_tag="ACHY5W_004479"
     CDS             complement(<1..491)
                     /locus_tag="ACHY5W_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449226.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFP"
     gene            complement(491..820)
                     /locus_tag="ACHY5W_004480"
     CDS             complement(491..820)
                     /locus_tag="ACHY5W_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449227.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRIRMLRVEFSA
                     EFKQVVN"
     gene            complement(817..3396)
                     /locus_tag="ACHY5W_004481"
     CDS             complement(817..3396)
                     /locus_tag="ACHY5W_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4449228.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYLGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDLTHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHEQETLEYKRQLADLGDKVEHQKRLNELAQ
                     QAARFEQQQGAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPAALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQN
                     WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSG
                     GRR"
     gene            complement(3377..3790)
                     /locus_tag="ACHY5W_004482"
     CDS             complement(3377..3790)
                     /locus_tag="ACHY5W_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4449229.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(3817..>4173)
                     /locus_tag="ACHY5W_004483"
     CDS             complement(3817..>4173)
                     /locus_tag="ACHY5W_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4449230.1"
                     /translation="IEHLALLKRRAEQAESSGNLQVSVEDLVRTGAFLVAMSLWHNHP
                     QKTASPSMNEAVMQIEQEVLTTWPADAIARAEDVVLRLSGMSGAVHADTDSTEVAKNN
                     ALTDDDFSAGKSSTAS"
BASE COUNT          811 a         1440 c         1020 g          902 t
ORIGIN      
        1 ggaaacagcg ccccgtccgt ctccgtcggt gttgccagca caaacgaggc tgtcatggcc
       61 gtcagctctg acatctgctc caccacccag cggtcgctca gctcctgctc cgggtccgct
      121 tccggattgc ccgccacaaa attcaccgca tccagaaaac gggcatacac ccggcggcgg
      181 accaccgtgg cccccaccag gctctgcagg tcctccgcca tcccggtgac cagaccaaac
      241 agattggaca ccgtcagcga cgggcgggca ctgctgccct tcccgttcat ctcaaagcca
      301 ctgccgtcaa tcgggtatgc ctgatattgc cgcccctgcc aggtaaccgc ctcccctttt
      361 tcattcagct cattgcagaa aaaataccgc tcaccaccct gtaccgtcag gtcgatttcc
      421 cagagtacca cccgcggtga ctgctctgat ttaaccgact cgttcagact ttcttcgtga
      481 atatcctgca tcagttcacc acctgcttaa actcggcact gaattcaacc cgcagcatgc
      541 gaatccgtga tgaccaggcg gcacaggtca cctttatctg ccgccaggca taaggcggtg
      601 tccacagaaa cgccttccag ccaccgtgct ctgccaggaa tgcctccaga tgtcgggcct
      661 cctcccgggt cacggaaagc gtcacacggt atgttttcag gtcagcattc agccccgccg
      721 ccatacgctg cgaatacccg tcaccaaaac gcacttcacg caccgatggc tgcgagttca
      781 cctccatatc cggcttcact ttccagcgaa atgttttcat cgcctgcctc cggaaaagac
      841 gccgccatca cgcatctgcg cctgaatctc atcctgcgcc cccttgcggg ccatgtcata
      901 caccgccttc atcagctgcg gccccgcctg tccgttggtg ccgtcgttct gaatcaccac
      961 gtgattgttc tgattaaaat taatgccttc cgtccgccgc atctgcgccg gacttccggc
     1021 accgcccaca taaccacctt ccgcatagcc ccgcatcagg cggtacaggt tgccgacgcc
     1081 aatccggctg gttgcttcct tcgtgaagac aaactccccg cgatgaacaa tccccgcagg
     1141 ttcatattta ccccccgtcc ccgtaaatcc cccggtcgcg aaatggaagt tcgccgccgc
     1201 agcctgaatg gccgtccccg tggaggcaga cgcaccacca ccgaaagcac cacccatggc
     1261 gctgccgata ctcccgacta tccccaccat cgcctgcttc agaaaaatct ctgtcagcat
     1321 ggagagcaca gaacgggtga aaccacgcca gttctgttcg ctgccggtca gcatcgctgc
     1381 catattctgt gcaataccgt caaaggtctg cgtggccacg cttttaacct gcgaaaaact
     1441 gtccgtcgca ctttctgccc actcgcccca gccggacttc agcccggcca tccagcttcc
     1501 acgaagctgc tcctccgcag accaggtgtt cttcagtgca gatgtggcct tcgccagcgc
     1561 agccggatta tcaccgtaca cgtcacgaag gcgctgctct tccgactccc gctgcgcctg
     1621 acggtcggtg aggccgcggg cttttgcgct gatggctgcc tgcttcgcgc cctgctgctg
     1681 ctcaaaccgc gccgcctgct gtgccagctc attcagccgt ttctggtgtt caaccttgtc
     1741 acccaggtca gccagctggc gtttgtactc cagcgtctct tgctcatggg ccagcaggga
     1801 tttctcctgt gcagacagct ggcgacgttg cgccgcctcc tccagtaccg cgaactgact
     1861 ctccgccttc cacaaatccc ggcgctgctg gctgattttc tcattcgctc cgctgtgctt
     1921 ctccagcgtc cggagctcgg tttcaagcgc cagcagcgcc gcatgcgtca ggtcttcctg
     1981 acgctcaccg gctgacactt tgactcctga cgacttcggc tttttcagcg tcgattcata
     2041 atcctttttc gccgccgcca tcagcgtgtt gtaatccgcc tgcaggattt tcccgtcttt
     2101 cagggcctta ttcagttctt cctgacgggc ggtatatttc tccagcggcg tcagcagacg
     2161 ctcatacgcc ttctgcgcct ctccggtata cttcagctgt gatgcgtccc gttcggcccg
     2221 gtccctggcg gccagttcac cggctttttc catatccgac tgcagcgtgg ccgctgccag
     2281 ccccagacgg gcattttccc ggtcattcca tgcgccctga aggttggccc ggaaagaggc
     2341 ggtttttccc cggcgctggc tccggctctg gtaccactgc cattttttat ccgcctcatc
     2401 aaatgccttc tgtgcactgg cgagcatatc cgctgaggac tcaggacgac cgatatccag
     2461 aatggcatcc cacatcgatt tgaacgcctt ccctgtttta tccgcccagg tctccagtgt
     2521 ccccatgttt tctttcaggc gacgggtctg ctcatcaaag cctttcgtgg cgatatcgtt
     2581 cgccgcctgt aaggccccgg cctcatcacc ggaacgctgc agctgcgcaa catacgcaat
     2641 ctgctctgcc gtcacgttac ggaactggcg cgccatcgca atcagccccg acgtcgggtc
     2701 ggtggtcagt tttccgaaag cctctgcaac cttgtccacc tccacaccgg atgcagaagc
     2761 aaaacgcgcg acactctggt tgatggcatc aaactgttca ccaccacgca caccggcatt
     2821 caccagggct gccagtgact ctctcgcctg gttaaacgtc agccctgcgg cctgtccggc
     2881 tctggagagc gtcagcatgc gatcggcagt cagtccggac tgattaccgg aaagaaccag
     2941 ggttttatta aacgctgaaa gcgtggaatc ccccaggtac caggcgtacg ccagcgcacc
     3001 tgtcgccacc gccagcgagg tgaccccgac catcggcagg gtgatcgcac cggcaagtcc
     3061 cctgaacatg gggatcatcc cgccgaagga gtccttcacc tgaccgccct gttgcagcag
     3121 gatcagccag ggattctgac caccggcaag ctgcgtggcg atatccgtaa actgtgcggg
     3181 cagggttcgc atggccgctt tatactgccc gacggaaatc ccggcttttt gtgcagccag
     3241 cgcctggcgg ctcaggccct gttcaacagc actggcggtt tttctgacgt cggtatccag
     3301 acctgaaaaa tgacgcctta cccggctcat ctgctcatcg aaacggaccg catccagact
     3361 caggtcaata acaagatcac caaccggctg ggacatatct cacacctccc ggaatccccg
     3421 ctgaagccat cattaatgcg gcatcatcca ccatgacatc cgccacatcc gcagacgata
     3481 aaatatcgcg ccctccgtcc ccaccgaacc ggacgcctcc ggcaagtcct gccgctttct
     3541 gcatcagcat tttttcctca tccggcatct ccgtctgcgc ttcctcacgc cggggggcaa
     3601 gcagactgaa atcagaggga tgcatatccg gatcgcaaaa aaacaggctg agtacggcgt
     3661 acgtcagccc ggaaaaatgc atatccagtt gggtatcgag aaaataatgc gtacggtaaa
     3721 aatgtcgcca gtcggcatat tcggtggatg tcatcccggc aagcatggcg cgccagtcag
     3781 gcctccccat ctcacgcgcc agtctgaggg caaaattcag ctcgccgtcg aagactttcc
     3841 cgcagaaaaa tcatcatcag tcagcgcgtt atttttcgcc acttcggtgc tgtcagtatc
     3901 cgcatgaaca gccccgctca tcccggacag acgcaacacc acatcttccg cccgggcaat
     3961 ggcatccgca ggccaggtgg tgagcacctc ctgttcgatc tgcatcacag cctcattcat
     4021 tgacggtgat gccgttttct gcggatggtt atgccacagg gacatcgcca ccagaaacgc
     4081 gccggttctg acgagatctt ccacgcttac ctgcaggttg ccgctggatt ctgcctgttc
     4141 tgcacgccgt ttcaggaggg caagatgctc aat
//
LOCUS       DAZUGK010000289         4166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZUGK010000289 DAZUGK010000000
VERSION     DAZUGK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.291"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..961
                     /locus_tag="ACHY5W_004484"
     CDS             <1..961
                     /locus_tag="ACHY5W_004484"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HFS4449231.1"
                     /translation="PDYFLNAAGKNAAETIGQVIIKVDEVLEIEKPEAILVLGDTNSC
                     ISAIPAKRHKVPIFHMEAGNRCFDQRVPEETNRRIVDHTADINMTYSDIAREYLLAEG
                     IPADRIIKTGSPMFEVLSYYMPQIDGSDVLSRLNLQSGEFFVVSAHREENVDSPKQLV
                     KLANILNTVAEKYNLPVIVSTHPRTRNRIREQGIEFHSNINLLKPLGFHDYNHLQKNS
                     RAVLSDSGTITEESSIMNFPAVNIREAHERPEGFEEASVMMVGLECERVLQALDILAT
                     QPRGEVRLLRQVSDYSMPNVSDKVVRIVHSYTDYVKRVVWKEY"
     gene            961..2172
                     /locus_tag="ACHY5W_004485"
     CDS             961..2172
                     /locus_tag="ACHY5W_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4449232.1"
                     /translation="MKLALIIDDYLPNSTRVGAKMFHELAQEFIQRGHDVTVITPGTG
                     MQEEISFDTFQGVKTWRFKSGPLKDVSKIQRVVNETLLSYRAWKAIKKWVKKETFEGV
                     IYYSPSIFWGYLVKKIKARCQCPAYLILRDMFPQWVIDAGILNAGSPIERYFRLFEKI
                     SYRQANRIGLMSDKNLDVFRKDNKGYPCEVLRNWASLTPTIIPKDYIPLRKRLGLEDK
                     TIFFYGGNIGHAQDMTNLMRLVRNMAAYPQAHFLFIGQGDEVELINSLASEWALTNFT
                     YLPSVNQDEFKFILSEMDIGLFSLSARHSSHNFPGKLLGYMVQSLPILGSVNAGNDLL
                     DIVNQNNAGLIHVNGEDDKLCQSALLMLHDIDVRRQLGSGANILLKEQFSVESAAQTI
                     EMRLEACNAIN"
     gene            2159..2557
                     /locus_tag="ACHY5W_004486"
     CDS             2159..2557
                     /locus_tag="ACHY5W_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFS4449233.1"
                     /translation="MRLIDNDQLDKLYDQAEQSERLRSHLIMHGSHQEKVQRLLIALV
                     KGSYVEPHYHKLPHQWEMFIVMEGQLQVCLYGRNGEVIKQFIAGDNIGISIVEFSPGD
                     IHSVECLSPRALMVEVKEGPFDPSFAKSFV"
     gene            2675..4081
                     /gene="gndA"
                     /locus_tag="ACHY5W_004487"
     CDS             2675..4081
                     /gene="gndA"
                     /locus_tag="ACHY5W_004487"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4449234.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGYKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         1152 a          861 c          969 g         1184 t
ORIGIN      
        1 acctgattat tttttaaatg cagcgggaaa aaatgcggcg gaaaccattg gccaagttat
       61 tattaaggta gatgaagtat tagaaatcga aaaacctgaa gcaatactgg tattgggcga
      121 tacgaattca tgtatttctg ccattccggc caaacgccat aaagttccta tatttcatat
      181 ggaagcaggt aaccgttgtt tcgatcaacg cgtgcctgaa gaaaccaaca gacgtattgt
      241 tgaccatacg gctgatatca atatgaccta cagtgatatt gctcgtgaat atctcttggc
      301 tgaaggtatc ccagctgatc ggatcataaa aactggtagc cctatgtttg aggttctttc
      361 atattatatg ccccaaattg atggttcaga tgtgctatcg cgtttgaatc tacagtctgg
      421 tgagtttttt gtagtaagtg cgcatcgtga agagaatgtt gattctccaa aacagctcgt
      481 aaagcttgcg aacattctaa atactgttgc tgaaaaatat aatcttccag ttattgtctc
      541 cacacaccca aggacacgta accgaatccg tgagcaagga attgaatttc attcaaatat
      601 aaatctactg aaaccattgg gtttccatga ttataaccac ttgcagaaga actcacgagc
      661 tgtgctttca gatagcggta ctatcactga agagtcatcc atcatgaatt tcccagcggt
      721 aaacatccgg gaagcgcatg agcgtccgga aggctttgag gaagcatccg tcatgatggt
      781 ggggttagag tgtgaacgcg tattacaagc gctggatatt ctggcaacac aaccgcgagg
      841 tgaagtccgt cttttacgtc aggttagtga ttacagcatg ccaaatgtgt cggataaagt
      901 tgtcagaatt gttcactctt acacagatta tgttaagaga gttgtctgga aagaatattg
      961 atgaaacttg ctttaatcat agatgattac ctgcccaaca gtactcgtgt tggtgcaaaa
     1021 atgtttcatg aacttgctca agaatttatc cagcgtgggc acgatgttac ggtaattact
     1081 cctggtacgg gcatgcaaga agagatttct tttgatacct ttcagggggt aaaaacatgg
     1141 cgttttaaaa gcgggccgct caaggatgta agtaaaattc agcgagtggt caatgaaacg
     1201 cttttgtcct atcgggcgtg gaaagccatc aaaaaatggg taaaaaaaga gacctttgag
     1261 ggtgtgattt attattcacc ttccatattc tgggggtatt tagttaaaaa aattaaagct
     1321 cgttgccaat gtcctgctta tcttatttta agagatatgt ttccacaatg ggtaattgat
     1381 gcaggaatcc ttaatgctgg ttccccaata gaacgctact ttcgtctttt tgaaaaaata
     1441 tcttatcgtc aggcaaatcg tattggactt atgtctgata agaatcttga tgtttttcgg
     1501 aaagataata aaggctatcc gtgcgaagtt ttgcgtaatt gggcatccct aacaccaacg
     1561 atcataccca aggattacat accactacgt aagcgacttg gcctagagga taaaaccatt
     1621 ttcttctatg gtggaaacat aggtcatgca caggacatga caaacttgat gcgacttgtg
     1681 agaaacatgg cagcatatcc tcaagctcat ttcctattta ttggccaggg tgatgaagtt
     1741 gaattaatta attcattagc ctctgagtgg gcattgacga atttcaccta tttgccctcg
     1801 gttaatcagg atgaatttaa gttcattttg tcggaaatgg atatcggctt gttttctctt
     1861 tccgctagac actcttccca taattttcct ggtaagttat taggctatat ggttcagtcg
     1921 ctacctattt taggtagcgt aaatgccgga aatgatttgc tcgacattgt caatcaaaat
     1981 aatgccggat taatccatgt caatggtgag gacgataaat tatgtcaatc tgcgctatta
     2041 atgttgcatg atattgatgt gcgccggcaa cttggttcgg gggcgaatat attgttgaaa
     2101 gaacaattct ccgttgagtc tgcggcacag acgatagaaa tgaggttgga ggcatgcaat
     2161 gcgattaatt gataatgacc aactcgacaa attatatgat caagccgagc aatcggaacg
     2221 tttacgttcc caccttatta tgcacggctc gcatcaagaa aaggtacagc gtttacttat
     2281 tgcattagta aagggcagct atgttgaacc gcattatcac aaacttcctc atcagtggga
     2341 aatgttcatt gttatggagg ggcaacttca ggtttgtttg tatggtagaa atggtgaggt
     2401 tataaagcaa tttatagcag gagataatat tggaataagc attgtggagt tttctccggg
     2461 cgatatacac agtgtcgaat gcctatctcc gcgtgctctt atggtggaag ttaaagaggg
     2521 gccatttgac ccttcttttg caaaatcgtt cgtgtgatgc ttgtctaaag tacatcttct
     2581 gctatctact caagctaaac ctgagttaac atccatacca tatttcaagc tgcgcatatc
     2641 ttgcgcggtg accaccccct gacaggagta tgtaatgtcc aagcaacaga ttggcgtagt
     2701 cggtatggca gtgatggggc gcaatcttgc gcttaacatc gaaagccgtg gttataccgt
     2761 ctctattttc aaccgttccc gtgaaaagac ggaagaagtg attgccgaaa atccaggcaa
     2821 aaaactggtt ccttactata cggtgaaaga gttcgttgaa tctctggaaa cgcctcgtcg
     2881 catcctgtta atggttaaag caggtgcagg cacggatgct gctattgatt ccctcaagcc
     2941 atacctcgat aaaggtgaca tcattattga cggtggtaat accttcttcc aggacactat
     3001 tcgtcgtaat cgtgagcttt ctgcagaagg ctttaatttc attggtaccg gtgtttccgg
     3061 tggggaagaa ggtgcactga aaggaccttc cattatgcct ggtggccaga aagaagccta
     3121 tgaactggtt gcaccgatcc tgaccaaaat cgccgcagtg gctgaagacg gtgagccatg
     3181 cgttacctat attggtgccg atggcgcggg tcactatgtg aagatggttc acaacggtat
     3241 tgaatacggt gatatgcagc tgattgctga agcttattct ctgcttaaag gtggcctgaa
     3301 cctcaccaac gaagaactgg cacagacctt taccgagtgg aataacggtg aactgagcag
     3361 ctacctgatc gacatcacca aagatatctt cactaaaaaa gatgaagacg gtaactacct
     3421 ggttgatgtg attctggatg aagcggctaa caaaggtacc ggtaaatgga ccagccagag
     3481 cgcgctggat ctcggcgaac cgctgtcgct gattaccgag tctgtgtttg cacgttatat
     3541 ctcttctctg aaagatcagc gtgttgccgc gtctaaagtt ctctctggcc cgcaagcgca
     3601 gccagctggc tacaaagctg agttcattga aaaagttcgt cgtgcgctgt atctgggcaa
     3661 aatcgtttct tacgctcagg gcttctctca gctgcgtgct gcgtctgaag aatacaactg
     3721 ggatctgaac tacggcgaaa tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca
     3781 gttcctgcag aaaatcaccg atgcctatgc cgaaaatccg cagatcgcta acctgctgct
     3841 ggctccgtac ttcaagcaaa ttgccgatga ctatcagcag gcgctgcgcg atgtcgttgc
     3901 ttatgcagta cagaacggta tcccggttcc gaccttcgcc gctgcggttg cctattatga
     3961 cagctaccgt tccgctgttc tgcctgcgaa cctgatccag gcacagcgtg actatttcgg
     4021 tgcacatact tataagcgca ttgataaaga aggtgtgttc cataccgaat ggctggatta
     4081 atctgactta actaacaata aattgaggcc cggcgtaaat tgtgccgggc tttttttgcc
     4141 aattatctta tagactaaat ttcact
//
LOCUS       DAZUGK010000290         4116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZUGK010000290 DAZUGK010000000
VERSION     DAZUGK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.292"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1791
                     /locus_tag="ACHY5W_004488"
     CDS             <1..1791
                     /locus_tag="ACHY5W_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4449235.1"
                     /translation="TEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHNAPQHVDKD
                     QHLPCDKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIQDNNNKTETF
                     SFTNLQWENLLQYYRENHQQPNIAESRNLTDNIDKIKNTISTSEIIECASPEIRSSVL
                     NDLYSIANFLPDNNLTPNESWKRFCDTCERFYVAQKSITGDKSERLTRKLSISDAGIT
                     MTFTIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTGIDLSKMVLRNVNFNGSILR
                     HAKFSGTICEGVDFTDCDLRNAKFKNASLENNDFRKVRHLTYVNFKNANLRNSNFSGK
                     VLTGVNFTGSNLSNAYLEYIDFTTVILYETPATPETAKIPEIPVTPQIPETPKVILTG
                     AILNYSDLSGKDLSKYNLTGIVCMYTNFSNTNLTNCELSNANFSNAKFYNTNCTGANC
                     SNILFDYAWLDNTIFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVKQANNSTDKQAN
                     DSTVQQSIDSTVQQANDSTDKQANNSTEKQANDNIDKQVNDSTDKQANNSTELLPQYR
                     FNQARLKEVVNIRFSIPGLTSYQPTYIVEE"
     gene            2058..2897
                     /locus_tag="ACHY5W_004489"
     CDS             2058..2897
                     /locus_tag="ACHY5W_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS4449236.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGNNKFVISDERDYAIYVISLNAESEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVTGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKS"
     gene            2968..>4116
                     /locus_tag="ACHY5W_004490"
     CDS             2968..>4116
                     /locus_tag="ACHY5W_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4449237.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GVFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLQPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALTSATLCGAAALFIAALICGMQ"
BASE COUNT         1198 a          942 c          853 g         1123 t
ORIGIN      
        1 accgagcaat tgcagcagtt tattaaaata atggaacaaa aagcgcaact accaatctat
       61 attgacaaga acaatttgaa agagagtatt ttctctgttt tgcacaatgc cccacaacac
      121 gtagataaag atcaacacct tccctgtgat aagtttttaa aacatgcctg caaaagttca
      181 aattcatttg aagtgaaatt agatgccact catcaatatc aacacctgaa taacttcatg
      241 atttcttttg acccagtaga aaatcaatta acaatacagg ataacaataa caagactgaa
      301 actttctcgt ttacaaactt acaatgggaa aatttgctgc aatactatag agaaaaccac
      361 cagcagccaa atatagcaga atcacgaaat ctcacggata atatagataa aattaaaaat
      421 acaatatcca cctctgaaat tattgagtgc gcctctcctg aaataagaag tagtgtcctg
      481 aacgatcttt atagcattgc taatttcctc ccggacaata atctgacccc aaatgagagc
      541 tggaaaagat tttgtgacac atgcgagcgc ttttacgttg ctcagaagag tatcactgga
      601 gataagagtg aacgtcttac gcgaaaactc tctatctctg atgcaggaat tacaatgacc
      661 ttcacgatag gtgatgttgt catcaatact attagcactg ctattcctga agatgcaacg
      721 gggcaacggt gtatcgaagg gttgaattta gcagaaatgg atttaaccgg catagacttg
      781 tctaaaatgg ttctaaggaa tgtcaatttt aatggcagca ttcttagaca tgccaagttc
      841 tccggtacga tctgtgaagg cgtggatttt acagattgtg atctgcgtaa tgcaaaattt
      901 aaaaatgcct cattagaaaa taatgatttt cgtaaagttc gccacttgac ttatgtaaat
      961 ttcaaaaacg caaatctacg aaacagtaac ttcagcggaa aagttctcac tggcgttaat
     1021 tttactggaa gtaaccttag caacgcttat cttgaatata tagatttcac aacagtgatt
     1081 ctatatgaaa cacctgcaac acctgaaact gctaaaatac ctgaaatacc tgtaacacct
     1141 caaatacctg aaacacctaa agtaattctt acaggagcaa tactaaatta ttcagatcta
     1201 tccgggaaag atctttcaaa atataatctt actggtattg tctgcatgta caccaatttt
     1261 tcaaacacta atttgacaaa ttgtgaactc tctaatgcaa acttttcgaa tgcaaaattc
     1321 tacaatacta attgtactgg tgcaaattgt tcgaatatcc tatttgacta cgcatggctt
     1381 gacaatacaa tatttataaa aacgcttttt aaaaatacct gtttttacaa tgtcagagcg
     1441 aaaaatgtct atcttgaggg agcatatctg aacaatgata atatcgtgaa acaagccaat
     1501 aacagtaccg ataaacaagc caatgacagt acggtgcaac aatccattga cagtacggtg
     1561 caacaagcca atgacagtac cgataaacaa gccaataaca gtaccgagaa acaagccaat
     1621 gacaatatcg ataaacaggt caatgacagt accgataaac aagccaataa cagtaccgag
     1681 ctcctccccc aatatcgttt taatcaagcc cgtttaaaag aagttgtaaa tataagattt
     1741 tccattccgg gtttaacgtc ttatcagcca acgtatatag ttgaagaata gttcaataat
     1801 cacccaaaca gagcaggtat ggcagaggat ataaattaac atttaaacgt taaccgcctc
     1861 atctatcatt catggatgag gcgcatttat cagtcgatgt aagctgaaaa tgttctgcca
     1921 tccagagaca accgccgccc ccctgcggcg tttactattt catcgcccgc gttaagaatt
     1981 cattaagctt catccgtata ctccttatct gtcgcactga atttgacgag aaaaaagcaa
     2041 taataaggag caacaagttg aaaaagcgca tcacctttat cgtttttctt tgcgccatcg
     2101 tggcggcaag cctgtttttt gtgcaatcct gcgtccgtaa aagccagcat gtcgcagggt
     2161 ttcagaatta tcaggccact atcgatggta aagagattac aggagtaact aaaaatatct
     2221 cctcactgac ctggtcagcg cagagcaata ccttatttag cactatcaac aaacctgcaa
     2281 ccatcgttga aatgacgacc gagggcgacc tgatccgcac cattccgctc gattttgtta
     2341 aggatctcga gacgattgaa tacattggta ataataaatt tgtcatcagt gatgaacgtg
     2401 actacgccat ttatgtgatt tcacttaacg ctgagtcgga agtaagcatc ctcaaaaaaa
     2461 ttaaaatccc attgcaggaa acgcctacta actgtggctt tgaaggactg gcttactccc
     2521 ctcaggacca taccttctgg ttttttaaag agaaaaatcc gatagaggtc tacaaagtaa
     2581 ccgggctatt gcgtagcgat gagttgcata tcagcaaaga taaagcgttg cagcgtcagt
     2641 ttactttaga cgatgtttct ggcgcggaat ttaacccaca gaaaaatacg ttgctggtat
     2701 tgtcacatga gtcacgcgcc ctgcaggaag tgaccgtgac aggtgacgtg atcggcgaaa
     2761 tgtcgttaac caaaggcaag tatggccttt cacacaacat caagcaggcg gaagggatag
     2821 cgatggatga cagcggcaac atctatatcg ttggcgaacc gaatcttttt tatcgcttta
     2881 cgtcgacaaa atcatgataa cagtctggca ggttctcctt ttttgttatt aacttgttaa
     2941 ctcaaaataa tacaaaccag gcagaacatg aattcgacat cgcatcccgt agaacgtttt
     3001 tctttcagca ccgcgttatt cgggatgctg gttctgacct taggtatggg tttaggccgc
     3061 tttctctata cgccgatgct gccagtcatg ctggcggaag gcgtgttttc ctttagcgaa
     3121 ctctcatgga tcgccagcgg taactatgcc gggtatctgg cggggagcct gctgttttca
     3181 ttcggcgcgt ttcatttacc ctcacgcctg caaccgttcc tgttagcttc cgctctcgca
     3241 accggattat taatcctcgc gatggcgtgg ctgccgccgt ttcttctggt tttcatcatt
     3301 cgctttctgg cgggggtcgc cagcgccggg atgttgattt tcggctcaac gctcatcatg
     3361 cagcataccc gtcatccctt tgtccttgcg gcgctatttt ctggtgttgg cgtcggcatc
     3421 gctctgggca atgaatatgt gctggcaggc ctgcattttg ccctctcttc acaaacattg
     3481 tggcaaggtg ccggagcact ttctgccatt atattgcttg ctctggcgct gctcatcccg
     3541 tcgaataaac acgttatccc gccagcgcca ttggcaaaaa tcgcgcaaca acccatgagc
     3601 tggtggttac tggcgattct gtatggtctg gcgggttttg gttatatcat cgtcgccacc
     3661 tacctgccgc tcatggcgaa agacgcgggc cagcctgtgc tgacggctca cctctggaca
     3721 ctggttggct tgtcgattgt cccaggttgc tttggctggc tgtgggcagc caaacggtgg
     3781 ggagcattac cttgcctgac cgcgaatttg ctggtgcagg cgatctgcgt gctgttaacc
     3841 ctcgccagca gctctccttt attactcatc atcagcagta ttggttttgg cggcaccttt
     3901 atgggaacga cctcgctggt gatgaccatc gcccgccagc ttagcgtgcc gggaaatctt
     3961 aaccttttgg gctttgtgac actcatttat ggtatcgggc aaattcttgg cccggcgctg
     4021 accagtatgc tcggcaacgg aacgtcggcg ctcaccagcg ccacactctg cggcgcagcg
     4081 gcgctattta tcgcagcatt aatctgcggg atgcaa
//
LOCUS       DAZUGK010000291         4067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZUGK010000291 DAZUGK010000000
VERSION     DAZUGK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.293"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..768
                     /locus_tag="ACHY5W_004491"
     CDS             <1..768
                     /locus_tag="ACHY5W_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFS4449238.1"
                     /translation="APVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMG
                     RHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIP
                     IKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFP
                     NNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISR
                     WQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            765..971
                     /locus_tag="ACHY5W_004492"
     CDS             765..971
                     /locus_tag="ACHY5W_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFS4449239.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            968..2569
                     /locus_tag="ACHY5W_004493"
     CDS             968..2569
                     /locus_tag="ACHY5W_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449240.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            2550..3869
                     /locus_tag="ACHY5W_004494"
     CDS             2550..3869
                     /locus_tag="ACHY5W_004494"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4449241.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            3879..>4067
                     /locus_tag="ACHY5W_004495"
     CDS             3879..>4067
                     /locus_tag="ACHY5W_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4449242.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILA"
BASE COUNT          908 a         1094 c         1286 g          779 t
ORIGIN      
        1 gcgcccgttc ctgaccgtgt ggcttacctg accgccggta tcgactccca gctggaccgc
       61 tacgaaatgc gcgtatgggg atgggggccg ggtgaggaaa gctggctgat tgaccggcag
      121 attattatgg gccgccacga cgatgaacag acgctgctgc gtgtggatga ggccatcaat
      181 aaaacctata cccgccggaa tggtgcagaa atgtcggtat cccgtatctg ctgggatact
      241 ggcgggattg acccgaccat tgtgtatgaa cgctcgaaaa agcatgggct gttccgggtg
      301 atccccatta aaggggcatc cgtctacgga aagccagtgg ccagcatgcc acgtaagcga
      361 aacaaaaacg gggtttacct taccgaaatc ggtacggata ccgcgaaaga gcagatttat
      421 aaccgcttca cactgacgcc ggaaggggat gaaccgcttc ccggtgccgt tcacttcccg
      481 aataacccgg atatttttga tctgaccgaa gcgcagcagc tgactgctga agagcaggtc
      541 gaaaaatggg tggatggcag gaaaaaaata ctgtgggaca gcaaaaagcg acgcaatgag
      601 gcgctcgact gcttcgttta tgcgctggcg gcgctgcgca tcagtatttc ccgctggcag
      661 ctggatctca gtgcactgct ggcgagcctg caggaagagg atggtgcagc aaccaacaag
      721 aaaacactgg cagattacgc ccgtgcctta tccggagagg atgaatgacg cgacaggaag
      781 aacttgccgc tgcccgtgcg gcactgcatg acctgatgac aggaaaacgg gtggcaacgg
      841 tacagaaaga cggacggcga gtggagttta cggccacttc cgtgtctgac ctgaaaaaat
      901 acattgcgga gctggaagtg cagaccggca tgacacagcg acgcagggga cctgcaggat
      961 tttatgtatg aaaacgtcct ccattcccac ccttctgggg ccggacggca tgacatcgct
     1021 gcgtgaatat gccggttatc acggcggtgg cagcggattt ggtgggcagt tgcgggcgtg
     1081 gaacccaccg ggtgaaagtg tggatgcagc cctgttgccc aactttaccc gtggcaatgc
     1141 ccgcgcggac gatctggtac gcaataacgg ctatgccgcc aacgccatcc agctgcatca
     1201 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     1261 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
     1321 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
     1381 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
     1441 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
     1501 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
     1561 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
     1621 atggacatgg ataccccgtg agttacccgg cgggcgtgcc tcgttcattc acgtttttga
     1681 acccgtggag gacgggcaga cccgcggtgc aaatgtgttt tacagcgtga tggagcagat
     1741 gaagatgctc gacacgctgc agaacacgca gctgcagagc gccattgtga aggcgatgta
     1801 tgccgccacc attgagagtg agctggatac gcagtcagcg atggatttta ttctgggcgc
     1861 gaacagtcag gagcagcggg aaaggctgac cggctggatt ggtgaaattg ccgcgtatta
     1921 cgccgcagca ccggtccgtc tgggaggcgc aaaagtgccg cacctgatgc cgggggactc
     1981 actgaacctg cagacggctc aggacacgga taacggctac tccgtgtttg aacagtcact
     2041 gttgcggtat atcgctgccg ggctgggtgt ctcgtatgag cagctttccc ggaattacgc
     2101 ccagatgagc tactccacgg cacgggccag tgcgaacgag tcgtgggcgt actttatggg
     2161 gcggcgaaaa ttcgtcgcat cccgtcaggc gagccagatg tttctgtgct ggctggaaga
     2221 ggccatcgtt cgccgcgtgg tgacgttacc ttcaaaagcg cgtttcagct ttcaggaagc
     2281 ccgcagcgcc tgggggaact gtgactggat aggctccggt cgtatggcca tcgatggtct
     2341 gaaagaagta caggaagcgg tgatgctgat agaagccgga ctgagtacct acgagaaaga
     2401 gtgcgcaaaa cgcggtgacg actatcagga aatttttgcc cagcaggtcc gtgaaacgat
     2461 ggagcgccgt gcagccggtc ttaaaccgcc cgcctgggcg gctgcggcat ttgaatccgg
     2521 actgcgacaa tcaacagagg aggagaagag tgacagcaga gctgcgtaat ctcccgcata
     2581 ttgccagcat ggcttttaat gagccgctga tgcttgaacc cgcctatgcg cgggttttct
     2641 tttgtgcgct tgcaggccag cttgggatca gtcgcctgac ggatgcagta tccggcgaca
     2701 gcctgactgc cggagaggca cccgcggcgc tggcgttatc cggtgatgat gacggaccac
     2761 gacaggcccg cagttatcag gtcatgaacg gcatcgccgt gctgccggtg tccggtacgc
     2821 tggtcagccg gacgcgggcg ctgcagccgt attcgggaat gaccggttac aacggcatta
     2881 tcgcccgtct gcaacaggct gccagcgatc cgatggtgga cggcattctg ctcgatatgg
     2941 acacaccggg cgggatggtg gcgggagcat ttgactgtgc tgacatcatc gcccgtgtgc
     3001 gtgacataaa gccggtatgg gcgctggcca acgacatgaa ctgcagtgca ggtcagctgc
     3061 ttgccagcgc cgcctcccgg cgtctggtca cgcagaccgc ccggacaggc tccatcggcg
     3121 tcatgatggc tcacagtaat tacggcgctg cgctggagaa acagggcgtg gaaatcacgc
     3181 tgatttacag cggcagccat aaggtggatg gcaaccccta cagccatcta ccgggtgatg
     3241 tccgggagac actgcagtcc cggatggatg caacccgccg gatgtttgcg cagaaggtgt
     3301 cggcatatac cggcctgtcc gtgcaggctg tgctggatac cgaggctgca gtgtacagcg
     3361 gtcaggaggc cattgatgcc ggactggctg atgaacttgt caacagcacc gatgcgatca
     3421 ccgttatgcg tgatgcactg gatgcacgta aatcccgtct ctcaggaggg cgaatgacca
     3481 aagagactca atcaacaact gtttcagcca ctgcttcgca ggctgacgtt actgacgtgg
     3541 tgcaagcgac ggagggcgaa aacgccagcg cggcgcagcc ggacgtgaac gcgcagatca
     3601 ccgctgcggt tgcggcagaa aacagccgca ttatggggat cctcaactgt gaggaagctc
     3661 acggacgcga agaacaggca cgtgtgctgg ccgaaacccc cggtatgacc gtggaaacgg
     3721 cccgccgcat tctggcagct gcaccacaga gtgcacaggc gcgcagtgac actgcgctgg
     3781 atcgtctgat gcagggggca ccggcaccac tggctgcagg taacccggca tctgatgccg
     3841 ttaacgattt gctgaacaca ccagtgtaag ggatgtttat gacgagcaaa gaaaccttta
     3901 cccattacca gccgctgggc aacagtgacc cggcacatac ggcaaccgcg cccggcggat
     3961 tgagtgcgaa agcgcctgca atgaccccgc tgatgctgga cacctccacc cgtaagctgg
     4021 ttgcgtggga tggcaccacc gacggtactg ccgttggcat tctggcg
//
LOCUS       DAZUGK010000292         4051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZUGK010000292 DAZUGK010000000
VERSION     DAZUGK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.294"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..3562
                     /gene="espI"
                     /locus_tag="ACHY5W_004496"
     CDS             <1..3562
                     /gene="espI"
                     /locus_tag="ACHY5W_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFS4449243.1"
                     /translation="NRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTVGKP
                     YYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVTLHN
                     YGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQGEKI
                     FSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTWTGA
                     GIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQPDAN
                     QKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAADYG
                     AEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYYLLK
                     SNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNTSVE
                     IPKLAGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLN
                     NLNLNNVDFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAS
                     GTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHK
                     GGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLK
                     ARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAP
                     SASATMNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMR
                     TDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQEI
                     GFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKR
                     MGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTH
                     TDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDY
                     STHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPL
                     IGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLM
                     SVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT         1179 a          835 c         1019 g         1018 t
ORIGIN      
        1 taaccggtat acggcttttt accgtgcggg cgttggctct caatatatcc gttatgcaaa
       61 tggcacagat aaactactgc aggcttacac tccagaaaag gcttatctga ccggcggaac
      121 agtggggaaa ccttattata ctcactataa cggtatgaag atgatttcag caaacccggg
      181 aaataccttt gataaaaacc agggacctct tgccagttat ggacagagtg gagacagtgg
      241 ttcgccgtta tatgcctggg ataacattga caaaaaatgg gtattagctg gagttactct
      301 gcataattat ggagtaaaag gtgcacgaaa tgactggctc ctgatacctc atgactttat
      361 cagtcaaaaa ttacaggatg accttaaacc aattattgtt gcttctccgg aggagaatat
      421 cttacgctgg gaatttgatc gttccagagg tacaggtact ctcagtcagg gagagaagat
      481 tttttccatg actggtagtg taaacggaaa tgcaaatacc gggaacaatc ttgtgttctc
      541 aggtaatgaa gggaaaatcg aactggtatc cagtgtggaa cagggagccg gatatcttca
      601 gtttgataaa gactacactg tactgacaaa taataacagt acatggactg gtgccggaat
      661 tattgtcggt gacgaggcaa atgtcaaatg gggagttaat ggcattgccg gtgataatct
      721 gcataaagtt ggttccggga cattaactgt taatggtcat ggtgagaata aaggtggcct
      781 taaagttggg gacggcgttg ttgttcttga gcaacagcca gatgcaaacc agaaacaaca
      841 ggcgtttagc catatcaata tagccagtgg tcgggcaaca gttaaactta acggggcaaa
      901 ccaggtagat gcagataata tcagctgggg atatcggggt gggaaactgg atttaaatgg
      961 gtatgatttt accttttccc gtcttcaggc tgcagattat ggtgctgaaa tcagcaacga
     1021 taatcagaca gataaatcca tagtcacact ttcgttatct cctctgaaag cagaagaaat
     1081 aaatgtggtt gttaataata taaatataat gggggggaca ggcaaaccag gtgatctgta
     1141 ttatacgacc tttgacggaa attattatct gttgaaaagt aaccgatatg gcagcgcttt
     1201 gtttggcgcg ctgaataatc agagcgaatg gcaaaggctg ggtaaggata aagaaaaagc
     1261 aattgggtta tatactcaga tgaaaatgca ggaaagcgct cctttatcat atatatatca
     1321 tggaaaaata accggtaata ccagtgtgga aatccccaaa ctggcaggca atgatatttt
     1381 aacgcttgat ggctctgtca gtatatcagg agatatgtca aaacaggacg gtgctcttat
     1441 cttccaggga caccctgtta ttcatgcagg gcaaactgtt tctgcatcgc agagtgactg
     1501 ggagaacagg gagttctcac tcaacaatct gaatcttaat aatgtggact tcagtctgtc
     1561 ccgtaatgca tttatgaacg ggaatatcag ggccgttaac cagagcactg ttattatcgg
     1621 cggagataca gtctttactg ataaaaatga cggaacaggt aatgatgtca tcagtgttga
     1681 agggaaatct gctgcctcag gaacatcctc ctatacaggg catatcactc tggagcaaaa
     1741 atcagcactg gatatccgcg ataattttcg tggcggggtt acgtctgaag acagtcatat
     1801 caatgtttct tcatcttcag tcctgttctc agatgcatcg tcatttataa acagctccct
     1861 gaatattcat aaaggaggtg cgctgaccgc tcagggaggg ctgtttacaa gtggaagcat
     1921 tgatattggt gacgcttccc ttctgcttac cggtacacca gtgaattcag atgatgctgc
     1981 ttttttaccg accatcaata tggctgatgg cggatttaaa ctgatgtctg attcatcagt
     2041 actgaaagcc agagaccagg catctgttgt tggtgatatt atttctgata aacaggccac
     2101 aatcagcttc ggaactgaat caggtaaaga gggcatatta tctgagaagg catcccgggg
     2161 actcgcggta ggattactga gtggttttaa tacggcatac cgcggtgcaa ttcatgcccc
     2221 gtcagcatct gccactatga acaacacctg gtggcaactg acaggagact cctcacttcg
     2281 ctcgttaaaa aataccggaa gcatgacata ttttacagga agtgcagcga ataaagcatt
     2341 ccatacactg acggttgatg agctgacgac gaatggcact gcgtatgcca tgcgtacgga
     2401 cctgaaaaat gcggataagc tggtagtaaa ccaaaagctg tcaggtaagg ataatattct
     2461 gctggttgat tttctgaaca aacccaccgg agaaaaactg gatattgaac tggtgagtgc
     2521 accggggaac agcagtaagg atgtttttaa aggaagtgaa caggaaatag gtttcagcaa
     2581 tgtcacacct gttattacag ctatagacgc cggagataaa acaacatgga acctgaccgg
     2641 gtacaggatg gcagaaaatc ctgccgcaac ccaaagtgcc tcaggccttg catctgtggg
     2701 gtacaaatca tttttgagtg aggtcaacaa cctgaataaa cgtatgggtg acctgcgtga
     2761 catcaatggt gaagctggcg catgggcacg tatcatgagc ggaaccggct ctgccggtgg
     2821 tggtttcagt gacaaccaca cacatgttca ggtcggtgtc gacaaaaaac atgagctgga
     2881 cggactggat ttgtttaccg gcttcactgt cacacacact gacagcagtg cctccgccga
     2941 tgctttcaaa ggtaaaacaa aatctgtggg ggccggactc tatgcttccg ccatgtttga
     3001 ttccggtgcc tatatcgacc tgattggtaa gtatgttcat catgataatg agtacaccgc
     3061 aacctttgcc ggactcggaa cccgtgatta cagtacgcat tcatggtatg ccggtgctga
     3121 agcaggctac cgctgtcatg tcactgagga tacctggatt gagccacagg cagaactggt
     3181 ttacggtgct gtatccggta aacagtttgc atggaaggac caggggatgc atctgtccat
     3241 gaaggacagg gactacaatc cgctgattgg tcgtaccggt gtggatgtgg gtaaatcctt
     3301 ctcaggtaag gactggaaag tgacagcccg tgccggtctg ggctaccagt tcgacctgct
     3361 ggctaacggc gaaaccgtat tgcgggacgc atcaggtgaa aaacgtatca aaggtgaaaa
     3421 agacagccgt atgctgatgt ccgttggcct gaatgcagaa atcagggaca acgtccgctt
     3481 tggactggag tttgagaaat ccgcctttgg taagtacaac gttgataatg ctgtcaacgc
     3541 taacttccgt tactcgttct gatactccgt attcaaaagg gcctgagggc cctttatgct
     3601 caggacctgc cgcccaggta tacatttccg gggtatcagt cgatttgttt acttaaccgc
     3661 catgaccaaa gccggcagca atgtcggctt tttacgtcca tatattccac tcatccgtaa
     3721 gcgtacagcc tgaaccgtct ggtcagaatc tgacgaatta gacgtaagcg tcgtctcagc
     3781 accgtctggc agatcctgaa attcctgaga gaatagtgga caccaaatat ggtggacgct
     3841 atccatgaaa tcattaactg cagtgcgtaa aaaaagtaag cgtaaactga ccgccgtatg
     3901 tagccatcag acgagaattg gtaacttaga cgcccatctg atatgtaagc gtacagcctg
     3961 aaccgtctgg tcagaatctg acgaattaga caaagtggtg tccaccaaat aagtagtggg
     4021 aaccaaagtg tcagatatgc agaaaaatgt g
//
LOCUS       DAZUGK010000293         3981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZUGK010000293 DAZUGK010000000
VERSION     DAZUGK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.295"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..589
                     /locus_tag="ACHY5W_004497"
     CDS             59..589
                     /locus_tag="ACHY5W_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS4449244.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(650..1678)
                     /gene="chuS"
                     /locus_tag="ACHY5W_004498"
     CDS             complement(650..1678)
                     /gene="chuS"
                     /locus_tag="ACHY5W_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFS4449245.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTAHGERQSIQFFDHQGYALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAKQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(1727..3709)
                     /gene="chuA"
                     /locus_tag="ACHY5W_004499"
                     /gene_synonym="shuA"
     CDS             complement(1727..3709)
                     /gene="chuA"
                     /locus_tag="ACHY5W_004499"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFS4449246.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGIRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTESSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1020 a          997 c          918 g         1046 t
ORIGIN      
        1 ctgttaatta cctttggcaa actgattata aagttaatgt ccgcaccagg agtcggttat
       61 gtttcttata attaccaggg atacgatgtt cttcaccgcg atgaaaaaca ttctgagtaa
      121 aggtaatgtc gttcatatac agaacgaaga agagatcgac gtaatgttgc atcagaatgc
      181 cttcgtcatt attgatacat taatgaataa tgtatttcat tctaattttc tcactcaaat
      241 tgaacgatta aaacctgtcc atgtcattat tttctccccc tttaatatta aacgctgcct
      301 ggggaaagtg ccggtgacct ttgttccgcg gactatcact atcattgatt ttgtcgcact
      361 catcaatggc agttactgct ctgtgcctga agcgaatgtg tcactttcgc gcaagcaaca
      421 tcaggtattg agctgcattg cgaatcaaat gacaacggaa gatattctgg agaaactgaa
      481 aatatcgcta aaaacgttct actgccataa acacaatatc atgatgatcc tcaatcttaa
      541 gcggatcaat gagctggtac gccatcagca tattgattat ctggtgtgaa cgattgatca
      601 atataaagag gcccagcaat aaatagccag acctcccgtt aattatacgt tatgcagcaa
      661 cgccttccgg tatcagcgaa gcaatttgct tacgccattg cgcttgctcc tgttcgcctt
      721 ctgtacgttg accataaagt tgcgctatct gcgtaccatc atgggcaaac aattccagac
      781 tggttacgta gccatcgctg gtcggtttac gggtaaccca ggcttcagca atgctctctt
      841 ctaatagatg aagagtaaac gtcgggttga aaatattcag ccaacctttc attggcacca
      901 ctttttctac cacaccggtg aaaatctgta cgcagccacg gttgccaaca aacaccatga
      961 tttcattacc atcctgcttt gcagattcaa gaatttgcgc caacgcactg ttggatactt
     1021 tgcaggccaa atcgtccgcc accagattga acgcctgttg gcgcgtcagg ttgtggcgct
     1081 tgagcaacgt aaaaaactga tgaacgtcgg tcatcgcccg ccactcttgc tcgaccacag
     1141 tggcatcggc tcgcgtttga acaactggcg catcaacggc ttttaactca agcggcgtat
     1201 tctcatcggt gataaaccgt gccagaagct cactccatgc cgccatatcg gtattgtcgg
     1261 tggcataaac ttttagtaat gcatagccct gatgatcaaa gaactgaata ctctggcgtt
     1321 cgccatgagc cgtgttttct ttgatgtgga aaacactggc ccattgattg agaaacagac
     1381 gtaaatccag cgcgcgtgga ttgaggatca atccggcatg tccgttcaaa tgctggtttg
     1441 tgaacgcacc aacttgctca tggactgcat attcattacg gcaaatacat ttggtctcgc
     1501 caacactttc gagcgccgcc agaatttcac ggatatcgcc gcgcatccgc cacgcatcgt
     1561 gcgtgacgcg tgcaaatgcc agttctgctt ctctaatatt cattaaccct gcgatgtcac
     1621 gcgcgtactt tccgggattt tgttctttta actcaagcca gcgtgtgtag tggttcattg
     1681 tctctcttcc ttccagataa atgccccgaa atatcggggc agatgattac cattgataac
     1741 tcacgaaaat ttttccgtta cgaccatcct gtgggatgcc ttgcggcgac cagtactctt
     1801 tgtcgaaagc gttacccaac accaaagtag tggtcatacc tttgagcgcc tgttgtcctt
     1861 gataactgac gtagaaatca ttcacgccat agcctggttg tttgctgtaa ctgctgctga
     1921 tatgtgttga gcgatcggca aacgtaccaa cccacccaac agagaagcca ctgtgagcga
     1981 tcggaatatt cagagtgctg gtaacggtat ccgggttaat gctggagatg tattcgccgg
     2041 tatcggtgtc tttgccgcgg gtacggttat aggccacatc aaggctaaac agatcagtgg
     2101 tatatttcgt catcacatcc cagccccaga ttttggcgtt cgggacgtta tacgacatag
     2161 tcgtcgccgc cgcgaaatcg acggtcgtgg agatgtaatc cttcgctttg gtatcaaagt
     2221 agctggcttt aaattccaga gcatcattgg acaacatcag gtcatcaaaa cgcagcccaa
     2281 aaccgtactc ctgagtttcg ttagtttccg gacgtaagtt cgggtttggc acccaatagt
     2341 tggtatagaa gcgaccaatc gagaagtgct tagaatcgtt atacatttcg cccatcgtcg
     2401 gggcgcggaa tgcctgggca tatgagccaa ataacatcag ccagttagtc gggttgatag
     2461 tcatccccgc acgagatgac catttgtcgg catcaacatc tttgtagccg tcgctgctac
     2521 cgcgataact gtcatagcgg gttccgccaa gcagggtaat cggcagatcg cgtaaggtga
     2581 tctcatcctg tagccagccg gagctaaaat cgatttttgc ttgcgggaag cccgtcgtcg
     2641 cgccgcccgg atgttgttcc tgacgataat actcaccgcc atatgtcagt aagtgagaag
     2701 cgaaactgtc ggcaaagaga gtggaacggt tctccagcct ggctcctttt gttatctgtt
     2761 cacgatactc gccggaactc tccgtgtttt gcgcattaat acggacttcc gaccaataaa
     2821 tttttgcatc tgcatttaac cagtcgttgc cctgcggggc gagtttataa gaaagctgcg
     2881 catcgcgttg aattgttgaa cgatcaacca tcgggttgct gctttcagaa gcctcaacgg
     2941 tctgcggatt ttttggttca cgcgcgtcgt tgttgtagta acgcactaaa ccgctcagag
     3001 actgggctga atcaatttgc caggtccctt tcgccagcat gttattaatg gactcgtcat
     3061 tcggcgcggt ttcaccattg ctctggcgta aatcaccccg atcgcgactg gaccaggcca
     3121 caataccatc cagattttca gttcgcccaa acgcgctcgc gcctaatccc aggctatggt
     3181 cccccgtgcc gccagtacca aagacacgaa aaccactgct ttgtccttcc tgcaataaat
     3241 cttttgcatc gaccgtatcg taggagatca ctccacccag cgcgccactg ccatacagta
     3301 atgctgaagg tccgcgaaca atctcaacac gcttgatcag cgccggatcg agaaaagtgc
     3361 cattcaggtg tccggtatcc gttccctgac gaataccatc gacaagaacc agcacgccgc
     3421 gatgatcata gccacgcata tttacatcct gaccgttggt tcgtccggta ccatccagag
     3481 taattccagg aacatgacgc agcagatcgg tggctgaagt agccgtttga ttttcaggag
     3541 cggaagtgtc gatgacgctg accatcatag gcgcttcgaa ggaactacgg gcattccccg
     3601 ttgccgtaac ggtcatggtt tcagtagcaa aagcaaacgt tggcaaggtg gcagaaacag
     3661 ccaaggccaa caaactcaaa cgcaacgagg taaattgcgg acgtgacata gcgattctcc
     3721 atgaggaaaa taaaaaatgc tggctgcctg ggttaatacc ggggtggctg gcgaaaaata
     3781 aatttattta aaattcagag cgataatatt cgccgggtga gtttacaggc gtttatatcc
     3841 catgaaattg cctgataact cggcaattac gcaaaagcac gccctctttg tcaggcaaaa
     3901 cgatatgccc catcaggagt gaagagagct gtgcgttatc ccttgcaaat gctatggttt
     3961 tgttatccat atggtcgtgt t
//
LOCUS       DAZUGK010000294         3974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZUGK010000294 DAZUGK010000000
VERSION     DAZUGK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.296"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..648
                     /locus_tag="ACHY5W_004500"
     CDS             4..648
                     /locus_tag="ACHY5W_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFS4449247.1"
                     /translation="MESLGIRLKKLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLALALGTTESYLLYGVSSPESSLVQKNPGIQIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVKEGDFAVSMPDDTMHNPSGSPSIPVGATVIMRPGEKYKNGSIVAVI
                     VPDPLTNEPSMTIKKLVIDGKLVYLAPLNPRYQSSLLTPECNIVAVAKGVQFSL"
     gene            922..1074
                     /locus_tag="ACHY5W_004501"
     CDS             922..1074
                     /locus_tag="ACHY5W_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS4449248.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNAN"
     gene            1464..1652
                     /gene="dicB"
                     /locus_tag="ACHY5W_004502"
     CDS             1464..1652
                     /gene="dicB"
                     /locus_tag="ACHY5W_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4449249.1"
                     /translation="METSLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIFGECQ"
     gene            1649..1837
                     /locus_tag="ACHY5W_004503"
     CDS             1649..1837
                     /locus_tag="ACHY5W_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS4449250.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            1930..>3974
                     /locus_tag="ACHY5W_004504"
     CDS             1930..>3974
                     /locus_tag="ACHY5W_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS4449251.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTSGEAVTGVSRDCDEQSDSQTENADKSESPDSNDDCPECEIPVSTL
                     SLTHRFLHLYFFGQEFDEKYKHHATPEQRKDVIRIEMDMEDGNIQSLLTAVRSHPELD
                     KLTNHHLGRLAHSVEKAFTHATTRRISPAEFDKFISTWMKTDYLDQGLLTKEWAKGNC
                     VSEINRTPSGANAGGGILTDRGEGFVHDDASVERDVAAGVLARSMDIDIYNPHPAHAK
                     RIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAIVVASVKEAPIGIEATPHRVTEYLNK
                     VLTETDHANPDPEIVDIACGRSSAPIPQRVTKEGKQDDEEKPQPSGAMADEQATTEAV
                     EPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELYEARKNIPPKNPVNADKLLAASHG
                     EFVEGISDPNDPKWVKGIETRDSVNHNQQETEQKGHKAEQHSPNAQQNEPETKQPEPV
                     AQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETFNEESQDEAREKDPEEMESAGLP
                     NKECTEGDQHANGNNETGETANLLIKVNGHREITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT         1202 a          935 c          934 g          903 t
ORIGIN      
        1 ataatggaaa gccttggcat taggcttaag aaactcagaa aagacaaagg tctgacccaa
       61 gttgaacttg gcaagctttc tggcgtaact ggggtaacaa taggatactg ggagaaagat
      121 ctaaacgaac caggtagcaa agctcttagt aaattagccc tagcactagg cactactgag
      181 tcctatctcc tgtatggagt atcatcacct gaatcatccc ttgtgcagaa aaatccaggt
      241 atccaaatcc catacctttc ttggggagaa gcgatttctt tcctaatatt aaaaggagag
      301 aaaactatgg gaaatgtcga caggatcacc acattctttg atgtcaagga gggagatttt
      361 gccgtttcaa tgcctgatga tactatgcac aatccatcag ggtccccaag cattccagtt
      421 ggagccacag tgatcatgag gcctggcgaa aaatataaaa atggtagcat cgtcgcagta
      481 atagtcccag atccactcac aaacgaacca tccatgacta taaaaaaatt agttattgat
      541 ggaaagctcg tgtacttagc cccccttaac ccacgctatc aatcatcctt acttacaccg
      601 gaatgtaaca tcgttgctgt agcaaaaggt gttcagttca gcctctagct cccgctttgc
      661 cctgtaatca gaggtcggct ttgtcggcct tttatcttaa aaatatttat attcaacttg
      721 acggcaaaac taaatattct tatattcatt acaacccacc ccgcttcaca gaatgcaggg
      781 caatacttcg agttacccgg cagtggtcag gggttaagta gccagcccga ggcgtatgaa
      841 catgacggcg ggaacacttt gtataacagc gcagcaggtt tttagttccg cgacccggcg
      901 ttaaggggaa atgaggtcaa catggatacc atcgatcttg gcaacaacga atctctggtg
      961 tgcggcgtct tccccaatca ggacggcacg tttaccgcga tgacgtatac caaaagtaaa
     1021 acgtttaaaa ccgaagctgg cgcgcatcgc tggttagcca gaaacgccaa ctgattagcg
     1081 ccagtaaaaa caggtttcca caggttaatg taccctgaaa agtcatggca taacacgaaa
     1141 gcgcacggag aggtttcttt gcatataagt cttgtcgtta aatttcttcg accgtgcgct
     1201 tccggttgtg tagccagtgc aacctcgtgg tgtacccatt ggcggcatcg gtttgttgct
     1261 gactgatgtc cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctatgcgcac
     1321 gcggaacagt taaaaccgta aagtggtatt ttacggggcg caacgggcat ccttctgtat
     1381 tccgacgtta attgttaact ggttaacgtc acctggaggc accaggcgcc gcatcaacaa
     1441 aattcattgt tgaggacgcg ataatggaaa cgtcactacc aaacgttaat acgtctgaag
     1501 ggtgttttga tattggtatt ctgctcagta accgggagtt tactgaagat gccattaaga
     1561 tgagaaaata tgagccttat cttctcaatg ataattccat actctccaga attgcccttc
     1621 ttgaacttgg tattttcgga gaatgtcaat gacttcagca tttgcactgg tgatgacggt
     1681 ttttcttata acgggtgaat cacagaatgt gattaccgga atttatgcca gtaaagaatc
     1741 ctgccatcag gcaagagacg agcaaaaaat ttccggtgaa tgtctcccgt taaacaaagt
     1801 atcgctgtac ctgaataacg aaataccggc tggataaccc gccagccata ttaacaccat
     1861 acccgttgat taagtatgcc agcaatggca gggattcgta caaccttaaa atagttatga
     1921 ggtttatcaa tgagcactga taaagaagaa attgcactgt attacgaagc caaaaatgac
     1981 aaagtcagaa aacgccttgg gattaaaggc ggtttttact ggcgcacagc aaaaaaatta
     2041 tcggttgcaa tatcacgggg tgttgtcgca atggacgatg ctggatttga cgaagaggat
     2101 ttcaaaaaac ctgttcgcgt gaatttgccc attgttaatg acctgccgcc tgaaggtgtg
     2161 tttgataccg aattctgcaa ccgctatgaa aaaggcggag aagatggcat cacaatgata
     2221 tttatagcgc cttccccctc agttcaggac aaaccagcca gcactgacaa taccagcggc
     2281 gaagctgtta caggtgtcag ccgtgactgc gatgaacaaa gtgactctca gacagagaac
     2341 gcagataaaa gcgaatcccc ggacagcaac gatgactgcc ctgaatgtga aatccccgtc
     2401 tccaccctga gtcttaccca tcgcttcctg cacctctact tctttggtca ggagttcgac
     2461 gagaaataca aacatcacgc cacaccagaa caacgcaagg acgtgatccg catcgaaatg
     2521 gatatggaag atggaaacat acagagcctg cttactgcag tacgttcgca tcctgagctg
     2581 gataagctga caaaccatca tcttggaaga ctggcgcatt cggtggaaaa ggcattcact
     2641 cacgcaacaa cacgccgtat cagcccggca gaattcgaca agttcatttc cacctggatg
     2701 aagactgact accttgatca ggggctgttg acaaaagagt gggcaaaggg aaattgcgta
     2761 tctgaaatca atcgcacccc gtccggcgct aatgctggcg gaggaattct taccgatcgc
     2821 ggtgaaggtt ttgtccacga tgatgcgtca gtggaacgtg acgttgccgc tggcgttctg
     2881 gcccgttcaa tggacatcga tatttacaat ccacatccgg cacacgccaa acgcattgaa
     2941 gaaatcgttt cagagaataa gccgcccttt tctgtttttc gtgacaaatt catcgccatg
     3001 cctggtcacc tggattattc ccgcgcgata gtagttgcgt ccgtgaaaga agcaccaatt
     3061 ggtatcgagg ctactcccca ccgtgttacc gaatatctga acaaagtact gaccgaaacc
     3121 gatcatgcca accctgatcc ggaaatcgtg gatattgcct gcggtcgctc ctctgcccct
     3181 atcccgcagc gtgtaacaaa agaaggaaaa caggatgatg aagaaaaacc gcagccatct
     3241 ggcgcaatgg cagatgaaca ggcaacgact gaagcagtgg aaccggatac aactgaacat
     3301 aatcaggaca cgcagtcgat ggatgctcag ccacagataa attctgttga tgcgaaatat
     3361 cagaaactgc gtgcagaact ctatgaagcc aggaaaaaca ttccacccaa aaatcctgtc
     3421 aatgcagata aattactggc tgcttctcac ggagaatttg ttgaagggat tagcgacccg
     3481 aatgatccaa aatgggtgaa ggggattgaa acccgcgatt cggtgaacca taaccagcaa
     3541 gaaacggaac agaaaggcca taaagcggaa caacacagtc caaatgcgca acaaaacgag
     3601 ccagaaacga aacagcctga accagtagcg caacaggaac cggaaaaagt ctgcacagcc
     3661 tgcggtcaga ccggcggcgg caactgtcct gactgtggcg cggtgatggg cgacgcaaca
     3721 taccaggaga cattcaacga agaaagccag gatgaagccc gggaaaaaga tccggaggaa
     3781 atggaaagtg ccggactccc gaacaaggag tgcaccgaag gcgatcaaca tgccaatggc
     3841 aataatgaaa caggcgagac agcaaatctc ttaattaagg tgaacggtca tcgtgaaatc
     3901 acatccacca gcaggacgtg tgaccatcta atgatcgacc ttgaaaccat gggaaaaaat
     3961 cctgatgccc cgat
//
LOCUS       DAZUGK010000295         3968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZUGK010000295 DAZUGK010000000
VERSION     DAZUGK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.297"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..866
                     /gene="nanC"
                     /locus_tag="ACHY5W_004505"
     CDS             150..866
                     /gene="nanC"
                     /locus_tag="ACHY5W_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFS4449252.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            886..1992
                     /gene="nanM"
                     /locus_tag="ACHY5W_004506"
     CDS             886..1992
                     /gene="nanM"
                     /locus_tag="ACHY5W_004506"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFS4449253.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     TGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2057..3037
                     /locus_tag="ACHY5W_004507"
     CDS             2057..3037
                     /locus_tag="ACHY5W_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS4449254.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPMTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPVNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALTKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            3160..3855
                     /locus_tag="ACHY5W_004508"
                     /pseudo
     CDS             3160..3855
                     /locus_tag="ACHY5W_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1119 a          909 c          944 g          996 t
ORIGIN      
        1 ttcacaggag accttataca aaaaatataa aataagctac ccgttgccaa agacactata
       61 agcctggcaa aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc
      121 tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat
      181 tattactgtg cttttcgtca ccattaattt ctcaggctgc gacactggac gtacgtggtg
      241 gatatcgtag tggaagccac gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa
      301 atggatggtg ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg
      361 aaaatgccgc actcaatgat gttcaggttg aagttaatta cgcgattaaa cttgatgatc
      421 aatggacggt gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac
      481 cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt
      541 acgactggaa agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc
      601 atcgttggga tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa
      661 cgacgctata cagcaaacag aacgattatc gctatgcaaa ccataagaaa tgggcgacgg
      721 aaaatgcatt tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg
      781 actacctgga ccgtcagggt gtttacaacg gcagagataa tttatcggaa aacagttatc
      841 gcattggtgt gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaacaata
      901 acggcgcttg ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact
      961 cctgtgccat ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta
     1021 ggtagcgcag gtacggcatg gtacaagctg gatacacagg ccaaagataa aaaatggaca
     1081 gcgttagctg cattccctgg cggaccaaga gatcaagcaa cctctgcatt tattgatggc
     1141 aatctgtatg tgtttggcgg cattggcaaa aacagcgagg gcttgactca ggtatttaat
     1201 gacgtacaca aatacaaccc caaaaccaat agctgggtta aattgatgtc gcacgcgccg
     1261 atgggcatgg cgggccatgt gacttttgta cacaacggca aggcttatgt tactggcggt
     1321 gttaaccaga atatcttcaa tggctatttt gaagatctca acgaaactgg aaaagattca
     1381 accgctatag ataaaatcaa tgcacactat tttgacaaaa aagcagaaga ttatttcttt
     1441 aataagtttc tgttgtcttt tgatccctct acacagcaat ggagttacgc tggcgaatct
     1501 ccctggtacg ggacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt
     1561 aatggcgaag ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt
     1621 aataatttaa aatggaataa gcttgctccc gtctcatcac cagatggcgt cgctggcggt
     1681 tttgcgggga taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca
     1741 cgagaaaatt accaaaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc
     1801 actgatattc atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt
     1861 cgggcttacg gagtatcatt gccctggaat aatagtctat tgattattgg cggtgaaact
     1921 gcaggcggca aagcggtgac ggattcagtt ttgatctctg tgaaggataa taaagtcaca
     1981 gtacaaaact aacgcttcag ggccccagta aaggggccct gctatcaatt tgtcatttca
     2041 attacgcgaa atttatatga acgcaataat atcgcctgat tattactatg ttcttaccgt
     2101 tgctggtcag tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga
     2161 tgcgcctcat cccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc
     2221 gtcatgtcac tttaacgaca ttattccaat gactcactgc ccacacgatg ttcaggatat
     2281 gcagggttat caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca
     2341 ggcactgcat attgcacgga aattactgcc ctttattcct gttaacgcag gggttctcat
     2401 cgttccatgt tgccgtggcg gatcggcttt taccgcaggc agcgaaggga catattcaga
     2461 acggcacgga gccagccatg atgcttgtcg ttggggaacg gatactccgc tataccagga
     2521 tttagtcagc agaacgcgag ccgcactgac aaaaaatccg cagaacaaat tcctcggcgt
     2581 atgctggatg caaggcgaat ttgacttaat gaccagtgac tacgcgtcac atcctcaaca
     2641 ctttaatcat atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa
     2701 taatattact gacgcgccgt ggttttgcgg cgataccacc tggtactgga aagaaaattt
     2761 ccctcatgcg tatgaagcta tttatggcaa ttatcaaaat aatgttttag ctaatattat
     2821 tttcgtcgac ttccagcaac aaggtgcaag agggctaacg aacgcgcctg atgaagatcc
     2881 ggacgattta agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac
     2941 ggcactgcga agcagtcatt tcagcgcggc agcccgtcgg gggattattt ctgacaggtt
     3001 tgtagaagca attttgcagt tttggcgcga aaagtgagcg ttcattaatt tgatttattc
     3061 gcatccatca gcgcttttaa ctcctccggc gtgcggcact ataacgactt ccacaacaag
     3121 gggccatttt cttataccac tttcagtgac catccgctta tgccaccacc ctctacgcaa
     3181 ctgtttgcag agcaccttcc caccgagtgg atacagcact tcctgacgct ttctgctcat
     3241 gcgaccgttc gtcgtcttaa cctgagcgtg gatggcgaag cggggatgaa cctgctggcg
     3301 cccgcagcgc tgtcacccag gcgcgtcagc gcgtgggggc cgccccggtg gaatggctct
     3361 tccgccagac cgcacagaca tggggcgcgg aacgttacct gaaggatgac tggcacggcc
     3421 tgcaactttt tgccattgat ggcgcacagt tcaggacacc tgatgaaccc gagctgcgtg
     3481 aatattatgg ctctgccaac acatccactg agcggcagag cgcctacccc gtcatgcgtc
     3541 tggtggcgtt aatgaatctg ggaatcacat tctactgaat gccatgaccg cgcctaccag
     3601 cggagcgata cggcgtgagg caacaaaagc ggcgtcagag atcagcttta agttcgcgtt
     3661 ccagtttatc gtgacagaaa tgatagtgct gggaaacacg gcgtcaccgg ggactatccc
     3721 gaaacgactg gagcatctgc gggggctctg gaagtggtgt tcataacaaa acgcccccga
     3781 ccatcaaggg cgagggcggt taagatatca aaaacccgtt atcctgtgaa acacagtgcc
     3841 gccccgctta agtgaacggc attgtgtttc tgagggtcct taattttccc tgattctctg
     3901 acgttcctta ccattgatat ttccttcatt cctcaccaga gaaataaaaa atcactatac
     3961 tcatcaaa
//
LOCUS       DAZUGK010000296         3920 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZUGK010000296 DAZUGK010000000
VERSION     DAZUGK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.298"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..376
                     /locus_tag="ACHY5W_004509"
                     /pseudo
     CDS             226..376
                     /locus_tag="ACHY5W_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(594..2684)
                     /gene="iha"
                     /locus_tag="ACHY5W_004510"
     CDS             complement(594..2684)
                     /gene="iha"
                     /locus_tag="ACHY5W_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFS4449255.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            2623..2805
                     /locus_tag="ACHY5W_004511"
     CDS             2623..2805
                     /locus_tag="ACHY5W_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449256.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            3464..3920
                     /locus_tag="ACHY5W_004512"
                     /pseudo
     CDS             3464..3920
                     /locus_tag="ACHY5W_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT         1009 a          981 c          875 g         1055 t
ORIGIN      
        1 caggctgtac gcttactgac aaaatggcga ttgagaaggt gcaattgact tatttggatt
       61 tggtcgtggc gaggtggtga ccctgcattc tgctaaccgg gttatgagtc aggagtcagc
      121 gatagacaaa gggacttatc tgaccgggcc cctggaaaaa tggcgggaga gaatgatacc
      181 ttcctgctga cgagaccgga ctttgagatg acgagcgata gtgcggcagt gatgtacagc
      241 ctgatcggta gctgcaaaac ttaacggaat cgagccggaa acgtggctac gccacgtgat
      301 cagtgtaatc aacacctggc ctgccaaccg catgaaagag ttgttgccct ggaacgtcac
      361 tctctctgta aactaatttt taccccacgt ccttcacggt gcgcttacac tctggacaag
      421 tatgtaaatg ataagttaaa aataatccgt gcatttcctg tgacgatatt agttgttggt
      481 aacgaaatct cattagcgga tcggaaatgc acttaaccta atacgttgcc actgttccgc
      541 caggagttgc agtggcaacg tattctaccg tcagtgatag cgttttgtta ttatcagaac
      601 tgatagttca gcgacatcca gtaatttcgc tcaggtatca catatcctgt tgttgatgat
      661 cccgtctgga agtaatcacc ggcatacagc gtactcttac cggcactgta caggctcacg
      721 tcactgtaat ccttgttgag caggttattc accgcagcat tcagcgtcag ggcatccgtc
      781 atcttccacg acagacctgc atccaccacc gtccaggctt tcaggtattc tcctttctca
      841 tcatacactt tcttctgtac agcgctcagt gacgaataat tctgggtgaa acgtggtgtt
      901 ttcccgcgat aacgggcacc cagccatgat gccacctctt cggtgatctg ccagttcagt
      961 ttcgcattca ccatgtgttc aggggtataa ctcagcggcg cacctttgtt atcaccatca
     1021 cgttgttcac ttcgggtcca ggtgtaattc agtgacagcg tgacatcctc tgaccacagc
     1081 ggcaatgtgc cggcaaattc cacaccgtgc aaccgggcct ttccgctgtt tacatagcta
     1141 ttggtgttat catttatgga ataagagaca atcttgttgg agaagtcagt cataaaacct
     1201 gtgacattgg cattcagacc ggcggggtta tcgtaataca ccccagcctc ataactgacg
     1261 ctctcttccg gcttcaggtc ggggttacca agtagatttg tttttccctg cccggacaca
     1321 ccactaatcc ctttatgtag ctgccccatt ctgggtgcct tatatcccgt ggtcacaccg
     1381 cctttcagcg tccaggcatc tgccacatcc cagaccagat atgcacgcgg actgaagtgt
     1441 cccccgaatt gctcatgatg ttcatagcgg ctgcccgcag tcagcgcaag tgcatccgtg
     1501 agatgccact catcctcagc aaataccgac cagcttttct gccggaaagt ttcacctgtg
     1561 ctggcaagga caactccgtc tttcatggac gagctctgaa actcgccccc caccgtaacc
     1621 agatgagatt ctcccagagg ggtaagcagt aatgaattca ggataaggtt cgattcctta
     1681 agctcccgcg gctgaccggc aagcccccat ttgtcgcgct tcagtacact gcgtacaagc
     1741 tcacgacctt tattttctgt ctcgttccag ttcagatacg atttccatgt tccgaaggtg
     1801 aaagtatgat catagccagc tgaaattttg tttcgctcat agcgcagggt ccggtcatat
     1861 ccccccgtca gactccccag ttgcccatcc cggttatcat aacgctgccg ggtggtatcc
     1921 atatcaaacc agagcacatc ctgctccgac gccttccagt caagacgtgc accaagatta
     1981 taattctgtg actccgtggg ataaggaata cgcgtggctg ctgtatcgct cagtgatgtg
     2041 accgatgaac cctgacgctg ttgtgtgcta ccgcgtacct gcaggctgac agaatcatcc
     2101 acaaggggac cactgctcca gaaattaaac tggctgctgt taccccattt gttgctttcc
     2161 tgcagattca gccctgcatt gacggaagag agccatttgt ctgcattctt tctggtaatg
     2221 atattcacca caccgcccat cgcatcagag ccatacagtg tggacatcgg ccccctgata
     2281 acctcaatac gctcaatggc ggccagaggg ggcatgaacc cggtattcat ggcagaaaaa
     2341 ccgttgggag tcacgtcact gcttccgccc tgacgaacac catcaatcag tatcagcgtg
     2401 taactggctg gcattcctcg gatgctgatt tccagccctc cggttttacc cgtaccactt
     2461 tcaacatcca caccctctac tgatctcaga gcctccgcca gatcgtggta ctggctggac
     2521 tgcaattcct cctggctaat cacagaaaca ctggcggctg cgttagtcag ctttttctca
     2581 tagccggatg ccgagacaat catcacatcc tctgcagcag ctatgctgct ggctgaaaat
     2641 ccgagacagg gaatgactac ggaagccaga gtggttattc gcatattact aatctccgat
     2701 catgttaacc ggcaagacaa tacacaaata caaacaataa tgagaataat ttatatttgc
     2761 cttttacaaa aaataaagat tattgacaaa accaaatata aataacgact gcctcggcat
     2821 gccagttaac agctatccgt atggcgtgtt cggttttgcg aataaaaaat ggttactgta
     2881 gaagcggatt tccaccacat gtcaaccatg aatggtcagt gatgcgagac gactgggatg
     2941 atttggtcga tgacttcttc cggcgaatac tgatagaaac attgaaatat tgtaagagaa
     3001 agcgttcaaa attctgtggc acgtgaaaaa ttctacagcg tcatcacctt attcagtcga
     3061 ccttcaaaat aaacgtttaa cagagacaat caccatttgg attgtccgct gaggaggcga
     3121 acaatttcct agacagcttt atcatgtcag cccacattgt tggcgctggc aatactaagg
     3181 acaaacgatg gggaagtagc tttttagcgt tattttcggg agtaaaaaac gtggtatctt
     3241 tccgtatgtc agttgtatct tgctggtatt cgtagtcgtg ataaggcata caacgatatt
     3301 tatcttgacg ttagtgaaga ataagttgtt ttgtgatcta tgttaatctg ggattacaat
     3361 ccgggcttga tacatcgcat cacgactttt attctcttca gaatagaata tgttactccc
     3421 cgctttacag gctcaggatt tgaattacaa attatacttt taagtgaaca ataaccaagg
     3481 gtatttctta tttcctgaaa ccaggcttgg cccatttcgg gatgagtttg aaaaaactct
     3541 tgacgcatac gatagtgatg aaataaacga aacaaattac gtcaacaaac tcaggcgtct
     3601 tgcctgacat gctcctgaca tcattgatat tcacgctcat ctggcatatg catttgcgct
     3661 ctttatgctg caattctggc gaatgttcat ttacagcgtc atcaggatgc cgttatactc
     3721 attgataaaa tactaacata taaccctgag aataatcagg gaacacgctg gttactgggg
     3781 gcggaattgt tacgtgctgg caagcatggc agggctctgt acgtattgac aggacatgtc
     3841 aatgaatttc ccccctactg gtacgagctt gctcttttac attttatgaa tgaggcatat
     3901 attaatgctg caacggcatt
//
LOCUS       DAZUGK010000297         3887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZUGK010000297 DAZUGK010000000
VERSION     DAZUGK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.299"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            47..1705
                     /gene="dpiB"
                     /locus_tag="ACHY5W_004513"
     CDS             47..1705
                     /gene="dpiB"
                     /locus_tag="ACHY5W_004513"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFS4449257.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKMVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHY5W_004514"
                     /gene_synonym="citB"
     CDS             1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHY5W_004514"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFS4449258.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHY5W_004515"
     CDS             complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHY5W_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS4449259.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1056 a          934 c         1009 g          888 t
ORIGIN      
        1 taattacatc cgtcaaagag gctcgggaca acccgcaagg aaaacaatgt tgcagcttaa
       61 cgagaataaa cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct
      121 gattctggtg ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt
      181 tgaggactat ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc
      241 ctccaatgac agtgtcatct ctgcggtgaa aacgcgtgac tacaaacggc tggcgaccat
      301 cgctaacaaa ttacaaagag ataccgattt tgattatgtg gtgattggcg accggcactc
      361 gatccgcctt taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca
      421 gggcgcgctg gagaaagggg agagctactt cattaccggg aaagggtcaa tgggtatggc
      481 gatgcgcgcc aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat
      541 tggctacctg gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg
      601 cgtgtttgtc gtgctgttag ggattctgat gttgctgtcg tggttcctgg ccgcgcatat
      661 ccgtcggcag atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga
      721 ggcgctgttt agttcggttt atgaagggct gattgcggtg gatccgcacg gttacattac
      781 cgccatcaat cgtaacgcaa gaaagatgct ggggctgagt tcccctggac ggcaatggtt
      841 gggtaaaccc attgcagaag tggtcaggcc tgccgatttc tttaccgaac agattgatga
      901 aaaacgtcag gatgtggtgg ctaactttaa cggtctgagc gttattgcca accgggaagc
      961 tattcgttca ggtgatgatt tgctgggggc cattatcagc tttcgtagta aagacgaaat
     1021 ttccaccctc aatgcgcaac tgacgcaaat aaaacaatac gtcgagagcc ttcgtacatt
     1081 gcgacacgag catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta
     1141 tgatcgcgtg ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag
     1201 cctgcgcgag gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg
     1261 cgcccgcgaa ctggggctaa aaatgatcat cgtccccggt agccagcttt cgcaactgcc
     1321 gccaggactg gatagtaccg agtttgccgc cattgtggga aatttacttg ataacgcctt
     1381 cgaagccagc ctgcgtagcg atgaaggaaa caagatggtt gaattattcc tcagcgatga
     1441 aggcgatgat gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg
     1501 agacaaaata tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat
     1561 tgggttgtac ttgattgcca gctatgtaac gcgctgcggt ggtgttatca ctctcgaaga
     1621 taatgatccc tgcggtacct tattttcaat ctatattccg aaagtgaaac ctaatgacag
     1681 ctccattaac cctattgatc gttgaggacg aaacgccgct ggcagagatg catgcggaat
     1741 atattcgtca cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc
     1801 gaatgatgat cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg
     1861 gtagagggat taatttactg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt
     1921 ttaccactgc agccagcgat atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg
     1981 attatctcat taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc
     2041 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
     2101 ttaatgctta tgctcgcggt gaacctaagg acgagctacc gaccggcatt gaccccttga
     2161 cgctaaacgc ggtgcgaaaa ctgttcaaag agccgggtgt gcaacatacg gcagaaacgg
     2221 tggcgcaggc actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca
     2281 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat
     2341 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
     2401 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
     2461 cagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
     2521 catcccggca accgcaacga ctacgccaga aacgggtgac agggtacgac cgaggtttga
     2581 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
     2641 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
     2701 cagcattgtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     2761 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     2821 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     2881 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     2941 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccatc
     3001 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggagcagt
     3061 ggtggtgatt tcactgacat ctaacatttc atgagaaatg tgctcttttt tatccagata
     3121 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     3181 tgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     3241 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc
     3301 tccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     3361 cagaccggtt gcggaagaga cggccagaga catcagacag gcgacaaaat aggcggcaat
     3421 catcagcagg taaggggagt taatatactg taatggtttt gacgccagct tgaccaccat
     3481 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     3541 gccgaggtcg ccgccgcggc tcattagcaa tattttaacg tattcaacga tatccgtggc
     3601 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactaat
     3661 aatcagcaat aacaggccac cgacaaataa cacgccagtg gcggaatacc ctttaatgat
     3721 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     3781 actttttcct gtctccaggc cccaaagtaa ataataaaaa attcctaaaa cttaaggaaa
     3841 aaatatgccc aataaattgg cggtgaatgc tgattaaaat caagaaa
//
LOCUS       DAZUGK010000298         3871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZUGK010000298 DAZUGK010000000
VERSION     DAZUGK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.300"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(200..868)
                     /gene="artM"
                     /locus_tag="ACHY5W_004516"
     CDS             complement(200..868)
                     /gene="artM"
                     /locus_tag="ACHY5W_004516"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFS4449260.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(868..1584)
                     /gene="artQ"
                     /locus_tag="ACHY5W_004517"
     CDS             complement(868..1584)
                     /gene="artQ"
                     /locus_tag="ACHY5W_004517"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFS4449261.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1591..2322)
                     /gene="artI"
                     /locus_tag="ACHY5W_004518"
     CDS             complement(1591..2322)
                     /gene="artI"
                     /locus_tag="ACHY5W_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFS4449262.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2340..3068)
                     /gene="artP"
                     /locus_tag="ACHY5W_004519"
     CDS             complement(2340..3068)
                     /gene="artP"
                     /locus_tag="ACHY5W_004519"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFS4449263.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3286..3801)
                     /locus_tag="ACHY5W_004520"
     CDS             complement(3286..3801)
                     /locus_tag="ACHY5W_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4449264.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          964 a         1031 c          982 g          894 t
ORIGIN      
        1 gcgacgctgg cgcgtcttat cagacctaca aatcatccag ctaatgtagg ccggataagg
       61 cgtccacgcc gcatccggca caggcaccgt gctgatgtct gatgcgacgc tggcgcgtct
      121 tatcagacct acaaatcatc cagctaatgt aggccggata aggcgtttac gccgcatccg
      181 gcatcagcac gcagctacat taattccgcc gttcaaatgc cagtgctttg cgctcgatca
      241 gacgcatcat cagcgtcagc aggccgttga cgaccagata aataatccct gccgcgccga
      301 acaccattac atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca
      361 gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag
      421 aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct tttttgctca
      481 ttcccagggc gctacaggac tgccactgac cttccgggat cgcacgaatt gcaccgtaaa
      541 acagctgcgt ggtatacgcc gcactgttca gcgacaacgc aatcagcgca cataaccacg
      601 gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcgggaac tgacccggcc
      661 cgtaataaat caggaagatc tgcaccagca gcggtgtacc ggtaaacagc gtgatataac
      721 cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaacg
      781 ccagaatcag tgccacaatc agcgaggcaa cggttagcgt caggctggtg tgcagccctt
      841 tcatcagttc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg
      901 caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc
      961 cgccacaatg taccaggtaa atggttcctg ggtacgagta gcgatgcttt tggtttgcag
     1021 cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca
     1081 ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa
     1141 gatagccgat ttcgacagcc ccagcgcctg gccggattcc cactgaccca tcggcaccgc
     1201 tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat
     1261 gacaccacaa aggaacggac tcacgtcgaa gttctcaatg tccatctgca ccgggatctg
     1321 cacgaaccca agattgatag tgaagccatc cgaaagcgtc agcagcagct gcgaggaacc
     1381 aaaatagata aacagcacca ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc
     1441 tgaacctgcc cacgcgacag gacgccattt tgccgactcc cataccgcaa agaacatcgc
     1501 cagcgccagt ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct
     1561 tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt
     1621 ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagctc
     1681 agtgttgccc tgacgtaccg cgatgccgag gccagtaccg aagtaatctt tatcggtcac
     1741 tttatcgccc actgccgcca gtttcgggtt atctttcagc cattcggtta ccactgcggt
     1801 gtcaccaaat acgctgtcga tacgaccatt ttgcagatcc agttttgcgt tctggtagct
     1861 gtcatacgga acggtggtga tttccgggtg cttatccata atgaacttct ggtgtgtcgt
     1921 cccgttctga acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg
     1981 ctgacccaca aacagggcag agttgtcata gtacggggtg gtaaacagca cctgcttttc
     2041 acgctccgga gtgatatcca tgcccgccat caccgcttct acgcgacgga atttcaggct
     2101 tgggatcagg ctgtcaaatg cctggttgga gaaagtacag gtcgcatcaa tctctttaca
     2161 cagtgcctgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc
     2221 aaacggagga taggaggctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag
     2281 actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct
     2341 tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg
     2401 ccttgttcta cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc
     2461 acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg
     2521 atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcaaacagc
     2581 agtacctgcg gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca
     2641 gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt
     2701 tctgcacggg ccagcgcctg atctttactc aaccccagta cacggcaggg cgcttcaatc
     2761 aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg
     2821 ttacgacgca aatcgcgaat cgctttgtct gagggtgttt tggtgaaatc gaaatggttg
     2881 cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac acgcagcagc
     2941 gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc
     3001 agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga
     3061 atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct
     3121 ttgacagaat aattatgcaa tatttctgct ttaaaagtta aaagcgaagc gcattattca
     3181 ctaaacatag cacaaaataa cgggggcggt gggcgatgag cataaatgtc ggcattcctc
     3241 acgaaatgcc ggacaattta cggggtttat tggttgatca aggcgttagc gattctcgat
     3301 ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc gcacatcgtc
     3361 gatcacccaa cactggcctt cctgaatcat taacacttca tcctgccagc cctggtcacc
     3421 ctgcttgaga tccacgcgca atggaatgtt acgggcatca cgattaggga tagtcgatgc
     3481 actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat cgctgcttaa
     3541 caattcacga tgactattat cgcggctggc atcgctaagt agggttgcca gtttgtcact
     3601 caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcatagaa
     3661 ttgttgggcc acgctgtcag gccccccctg gacacaggaa ccggtgcggg gaccgttatc
     3721 tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag
     3781 agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac
     3841 gaccaaacgc tatcagatta tttaatacat t
//
LOCUS       DAZUGK010000299         3848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZUGK010000299 DAZUGK010000000
VERSION     DAZUGK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.301"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..260
                     /locus_tag="ACHY5W_004521"
     CDS             33..260
                     /locus_tag="ACHY5W_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4449265.1"
                     /translation="MRVDELVQFFGSVQRVADFYGVTREAIYMWRKRPGEIVPKGRAA
                     EAVAYSKGKLSLNPELYKKKDNTSNERKNDS"
     gene            257..682
                     /locus_tag="ACHY5W_004522"
     CDS             257..682
                     /locus_tag="ACHY5W_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449266.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            705..1673
                     /locus_tag="ACHY5W_004523"
     CDS             705..1673
                     /locus_tag="ACHY5W_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449267.1"
                     /translation="MSSKLHGLVWEGCAFSGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVGPSNFDGSTVDKKLLVRGAMIDPDPSVLK
                     PDPSDKRSSCPDVSLPDEKQSSPVERFLEKHPDAHTWNVPKRQWGTREDVACAQWIWG
                     RVVALYEQAASDDGEVSRPREPNWTTWANDVRMMRMLDGRSHRQICEMFGRVQRDSFW
                     VKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1680..2426
                     /locus_tag="ACHY5W_004524"
     CDS             1680..2426
                     /locus_tag="ACHY5W_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4449268.1"
                     /translation="MKNIAAAGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGGT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGSSVIIVTVSDVMSVLHDSYDNGQSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            2448..3164
                     /locus_tag="ACHY5W_004525"
     CDS             2448..3164
                     /locus_tag="ACHY5W_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4449269.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGLQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCMPGDNLPAEPEAASAAETDGKAFPQPAGVALPVQETT
                     TQEEMKTEIEEDIVKLQPSVTETKADDLILPSLHVANRELRRAKSDVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            3161..3478
                     /locus_tag="ACHY5W_004526"
     CDS             3161..3478
                     /locus_tag="ACHY5W_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4449270.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMKVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            3475..3780
                     /locus_tag="ACHY5W_004527"
     CDS             3475..3780
                     /locus_tag="ACHY5W_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS4449271.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEVLKREGHTVLNPAVLPD
                     GLAQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
BASE COUNT         1034 a          828 c         1162 g          824 t
ORIGIN      
        1 atctaaaaat acttagtttt aaggagggcg aaatgcgagt tgatgaactt gtccagtttt
       61 ttggttctgt ccaaagagtc gctgattttt atggggtaac ccgagaagct atatacatgt
      121 ggcgtaagcg tcccggcgaa atagttccca aggggagggc tgctgaagca gttgcatact
      181 ccaagggaaa attatcgttg aacccagaac tttacaaaaa gaaggataac acctcgaacg
      241 aaaggaaaaa tgattcatga aaatcaaaca tgaacacatc cgcatggcga tcaatgcctg
      301 ggcatatcct gatggcgaga aagttcctgc agctgaaata gcccggactt atttcgaact
      361 ggggatgacg ttcccggaac tgtatgacga cagtcatccg gaagccctgg cccgtaatac
      421 ccagaaaatt ttccgttggc tggataaaga cacccctgat gctgttgaaa aaattcaggc
      481 tctgttaccg gcgatcgaaa aggcgatgcc gcctttgctg gtggcccgta tgcgcagcca
      541 cagttctgag tattaccgtg agatcgtcga acggcgggat cggctggtga aggatgtcga
      601 cgattttgtt gcgtcagcgg ttgttttgta tgaccagatg aatcgcggcg gcccggcagg
      661 gaatgctgtg gtgatgcact aaaagcacgg tgttcggggg ttttatgagc agcaagcttc
      721 atggtcttgt ctgggaagga tgcgccttca gcggcatgat cttatccagg gtggcagtta
      781 tggcccgtct tgcagactac agcaatgacg agggcgtgtc atggcctgcc attgaaacta
      841 tccgtcgtca gatcggtgcc agaagtgaat ccacagtgaa atcggctatt gcagaactgg
      901 cgaaagaggg ctggctgacg aaggaagagc gtaaggtcgg tgggcgtaat gtaagcaata
      961 tctatcggct taatgtggaa aaactcgaag cagctgcggc ggcggcgcgt gagtcatata
     1021 aaccgaaaag aaaaattagc ccggcaaaaa atgacccgtt aacagttgac ccgtcaaata
     1081 ttgacccctc aacggttggc ccgtcaaatt ttgatggatc aacggttgat aaaaaactgc
     1141 tggtcagggg ggcgatgatt gaccccgatc cgtcagtatt aaaacctgat ccgtcagata
     1201 aaagatcttc ttgtccggac gtttcactgc cggacgaaaa acaatcatca ccagttgagc
     1261 gatttctgga gaaacacccg gatgcgcata cctggaatgt accgaagcga cagtggggta
     1321 cccgtgagga tgtcgcctgt gcgcagtgga tttggggacg ggttgtggcg ttgtatgaac
     1381 aggccgccag tgatgatggt gaggtgtcac gcccccgaga acctaactgg acgacctggg
     1441 cgaatgatgt gcgcatgatg cgtatgctgg atggacgtag tcaccggcag atttgtgaaa
     1501 tgtttgggcg tgtccagcgg gattcgttct gggtaaaaaa catcatgagt ccggcaaaac
     1561 tccgggaaaa atgggatgaa ctggttatcc gcctggggcg ttcgcccgcg cagcgttgcg
     1621 tgaatcacat ttctgaaccg gatactgaaa taccgcctgg attcaggggg tgacgtggca
     1681 tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca ccacaggggg
     1741 cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcagcttgct gaaggacgaa
     1801 aacgcagcga ggagattaac cgccagaatc gccagttgcg ggtggaaaaa atcctgaatc
     1861 gttcgggcat ccagcctctg cacagcaaat gctcgtttgc aaattatcag gtgcagaacg
     1921 acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg atgaccgggg
     1981 gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt gcagcggcga
     2041 tgggcaaccg gctgatggcg aaggggagca gcgtgattat cgtcaccgtg tctgacgtca
     2101 tgagtgtgtt gcatgacagc tacgacaacg gccagtccgg ggaaaaattt ttacaggagc
     2161 tttgcggggt tgatttgctg gtcctggatg aaataggcgt tcagcgtgag acgaaaaacg
     2221 agcaggtggt attgcaccag ataattgatc gccggacagc atcactgtgc agtgtcggga
     2281 tgttaacaaa cctgaatcat gccgcaatga gtacgcttct cggtgagagg attatggacc
     2341 gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg cgtccaaatg
     2401 tcagcaatca gagggttgtg aagtaatttt tgtcggagga cgttttaatg gaaaccgtat
     2461 ttgacgcact gaaagcactg aaaaaagcct cttcacaggt agtggcatcg cgccttggaa
     2521 tcagccgtga agatgcggtc aacgaactgt ggaaactgaa gcgccgcggt gaagcggata
     2581 acaagggttc gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg tcaccggtac
     2641 cgaaagtgac agcgcaaatg ctgacagagg cgattgaaca acatggccta caaacggcgg
     2701 atgaactggc actgatgttc ggaattacct cccgccgggc gaattcatcg ctggcaatgg
     2761 caatcagcaa agggcgtctg attcgcgtga atcagggcgg taaatttcgt tactgcatgc
     2821 cgggcgataa tttaccagca gagccggaag ctgcatccgc agcggaaacg gatggtaaag
     2881 cctttcctca gccagcagga gttgcgttac cagtccagga aacgacgaca caggaagaaa
     2941 tgaaaacaga aatcgaggaa gacattgtga agttacagcc atcggtcacc gaaacgaaag
     3001 cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc cgggcgaaaa
     3061 gtgatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac aaacacaggg
     3121 atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct ggaagaagtg
     3181 gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgaaag tcgaaagcat
     3241 cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg aactgggtat
     3301 cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc gtgatattga
     3361 gctggcgcgg caactgcatc agcgtggtgt accccgacgg gaaattgccg aaaagtttgg
     3421 gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc aggagtgagg
     3481 gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg cgaggcattt
     3541 cacaaagcgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc ggcagtactt
     3601 ccggacgggc tggctcagcc acattacatg gatatttgca tggcaatgat tcgttgtgtg
     3661 gatgcggttt atatgctgaa aggctggcag cggtcggcag gcgctaaggc agaactggca
     3721 ctggcggaga aactgggaca tgcggttatt ttccaggagg caaccagtga gcgaaattaa
     3781 ttaccaggaa ctacggcagt tgaaagagaa agcataatcc agaactgaat aattaaaatc
     3841 agcactgt
//
LOCUS       DAZUGK010000300         3802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZUGK010000300 DAZUGK010000000
VERSION     DAZUGK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.302"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            259..1410
                     /locus_tag="ACHY5W_004528"
     CDS             259..1410
                     /locus_tag="ACHY5W_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449272.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            1494..2366
                     /locus_tag="ACHY5W_004529"
     CDS             1494..2366
                     /locus_tag="ACHY5W_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS4449273.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGPREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVMQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHRVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            2584..>3802
                     /locus_tag="ACHY5W_004530"
     CDS             2584..>3802
                     /locus_tag="ACHY5W_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFS4449274.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVI"
BASE COUNT          979 a          978 c          983 g          862 t
ORIGIN      
        1 ccggagaata tcagtcaagc ttaaaaagac aataaataca gtgagttaaa tacacagaga
       61 aaacgtagta tcagagggta catcttacaa cagagactga atttccggtt tttcataacc
      121 ggcgcattcc ggaaagtgca ggcggcccgg aggttttctg acgccactac ctggtagatt
      181 gttctgtaca tcatgtacgg aggtttttgt ttgctttttt aaaatctgca aatgtgagaa
      241 aagcaggtga ggtgtattgt gggaaaggaa ctggatgata tctgcaccag ctgtccgtat
      301 atcgatgctg taaaacggca caaacaacag ctcggtgcca tagaggaata tacacagtgg
      361 ttaaaaaaag aaccacgggc atcttatttt tttctgttcc agctgtatac ccgcattcac
      421 aatacatttt tctcccaaaa gcaacaactc ccgttcactc ccggtggcac tcactgcccg
      481 gaacctgatg tcacccttcg ggacttaacg ctttcccctg gctaccacag tgattatgcc
      541 cctcaaccca ttccggagat ggacagttct gctgttgttc ccccaactaa tgaaaacacc
      601 tcgcctccgg aagatactcc cgacaacact ccggccggag gcaataccgg acaagcggaa
      661 aagaccagaa acgcaggact cacacccatt cctgaaaaac gttcaggaat gcccccggaa
      721 catctgcggt ttgccacggg gtttccatcg caaccgaaga ttgccggtcc cagagaaaag
      781 ccgatgcgca ccgtgcatcc ggataaaatc taccgtgaaa ttatatggtt ctgctccagt
      841 tacctgctga aatccggacc agaggctacg aggacgatta tcaactcagt attctctgaa
      901 tgggccagca taaacaatga ttacccctcc cccttttcgt gggtggacag cagggacagt
      961 gaacagtgtg actggttatg gaacgccatg caggtccggt gtgtgggaac cccgctgaat
     1021 ccccttaccc cggagcagaa atactggttt gcctgcgcca cgtttgataa ctgggagggc
     1081 tggaatgagc aacaggtaca gtttttactg gaaagtaatc ccagacggaa cagagcgaag
     1141 tttacgcagg tctccttcca ggcccccagg attcagcata aagcgattct tcttgatgag
     1201 ctgaaaagtg cccgggagca acaaaaaagg cgtgatgaac gtgctgatgg ttccgtccct
     1261 ctgaaactgt ccggaaaaat ccacaaacag cttgaaagta ttgcccgaag tcgtggtgtc
     1321 ctcccaaaaa aactgctgaa tgaaatgatt gagcaggcgt accatgattt ggtggcaacc
     1381 aggcagaaca gccaaatcga ttccagataa tttcagacag atattatttc cgtgaatccc
     1441 ccgccacctt tccggtgcgc ggggttttgt cttttttcac cgggaataca tgtatgaatc
     1501 cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac tcactttccc
     1561 gtcacatcct ggaacatctg cgcaaactca ccagtcacga acccgtgcct ggcattatgg
     1621 gtaaaagcgg ggccggtaaa tcctcactct gtaatgcgct gtttcagggg gaggtcaccc
     1681 cggtcagtga tgttcacgcc ggcccccggg aagtgcagcg cttccgtctg aatggccatg
     1741 gtcacagcat ggttatcact gacctgcccg gggtgggcga gagccgggac agggatgcag
     1801 agtatgaagc cctgtaccgt gacattctgc tcgaactgga cctggtactg tggctgatta
     1861 aagccgatga ccgtgccctg tctgtggatg agtatttctg gcgacacatc ctgcaccggg
     1921 gacatcagcg ggtactgttt gtggtgatgc aggccgataa aacggagccc tgccatgaat
     1981 gggatatggc cggtattcag ccttctcccg cacaggcaca gaacattcgc gaaaaaacgg
     2041 aggcggtatt ccgtctgttc cggcccgtac atcgggttgt tgccgtatcg gcccgcaccg
     2101 gctgggaact ggatacgctg gtcagtgcgc tcatgacagc acttcccgac catgccgcca
     2161 gtcccctgat gacccgcctg caggacgagc tgcgcacgga gtctgtccgg tctcaggccc
     2221 gtgaacagtt taccggtgcg gtggaccgga tatttgacac ggcggagagc gtctgcattg
     2281 cctctgttgc acgcacggtt ctgcgcgccg tccgtgactc ggtggtctct gttgcccgcg
     2341 cggtatggaa ctggattttc ttctgaaccc ggtggtgatg taccaggctg ttgttacaga
     2401 tttttaactc catgaaaaca ctatgcaaac gcagccatac ccgggttgtc cccttgcgtt
     2461 gtgtttctca agttaataga atacatcgat cgttactatc atatcgatcg ttataaacga
     2521 tcgtttgtag taaatgacct cctccgtggt tgtcatgaaa atgcgtctta ggaaggttaa
     2581 ataatgcacg aaaagaacat cgccctgctt tgtgatgaag ccgaccgact tttgcaactg
     2641 aacattaatc tgctccggca aatggttgag gagccagatg tgttatctga cagtaagaac
     2701 gaaaacagac tgctttttga taaacagaaa gcactgaaaa gaattgagga gctggagggc
     2761 gaacaaatca aaaccgcccg cagggagatg gtgctggctg ttgtcggcac gatgaaagca
     2821 ggcaaatcaa ccaccatcaa cgccattgtg gggcaggaaa ttctgcctaa ccgtaaccgc
     2881 cccatgacct ctgtaccgac gctcatccgc cacgttcccg gaaaaactga gccggttctc
     2941 catctggaac atattcagcc tgtccgcaat ttattaatca cactgcagga aaaactcgcc
     3001 accccggcag gacagcaggt cgcacagacc ctgcagcaaa ccggggatac ccgcgaactg
     3061 ctggatattc tgacggatga tggctggctc aaaaatgaat accacgggga ggaggaaatc
     3121 tttaccggac tggcatcgtt aaacgatctg gttcgtcttg ctgcggcaat ggggactgaa
     3181 tttccttttg atgaatacgc agaagtgcag aaactgccgg tgatcgacgt ggaattcagc
     3241 catctggtgg ggatggatgc atgccaggga acactcacac tgctggatac ccccggccct
     3301 aatgaggccg gacaaccgca gatggaagtg atgatgcggg atcaactgca gaaagcctct
     3361 gcggttctgg ctgtgatgga ttacacccag atgaactcaa aagcggatga agacgtccgt
     3421 aaagagctta atgccattgc tgacgtatca gccggccgcc tgtttgtact ggtcaataaa
     3481 tttgatgaga aagaccgcaa tggcgatggg gcagatgccg tacgccagaa agttccggca
     3541 atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc ccggatcctc acgccaggca
     3601 tacctggcta accgtgcgct tcatgagtta cggaaaaacg gaacccttcc tgttgatgaa
     3661 gcctgggtcg atgattttat cagaggtgcc ttcggttgca tgaaaaaaga atacgtctgt
     3721 aaagacagtg aactggcaac tgaaggggcg acagacctgt gggaaggctc acttatcgat
     3781 caactgataa cggaagtcat ac
//
LOCUS       DAZUGK010000301         3801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZUGK010000301 DAZUGK010000000
VERSION     DAZUGK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.303"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..1212
                     /gene="rfaQ"
                     /locus_tag="ACHY5W_004531"
     CDS             154..1212
                     /gene="rfaQ"
                     /locus_tag="ACHY5W_004531"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFS4449275.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1209..2333
                     /locus_tag="ACHY5W_004532"
     CDS             1209..2333
                     /locus_tag="ACHY5W_004532"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4449276.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2326..3123
                     /gene="rfaP"
                     /locus_tag="ACHY5W_004533"
     CDS             2326..3123
                     /gene="rfaP"
                     /locus_tag="ACHY5W_004533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFS4449277.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3139..>3801
                     /locus_tag="ACHY5W_004534"
     CDS             3139..>3801
                     /locus_tag="ACHY5W_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4449278.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQ"
BASE COUNT          990 a          831 c          934 g         1046 t
ORIGIN      
        1 aaatcattcg ggtcggattg ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc
       61 ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc
      121 ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg aagaattttg
      181 ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat tagttcgctg
      241 aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac catcccgatc
      301 ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa agcaaaagcc
      361 tcagaaaaaa ttaccaactt ttttcatctc atcaaggtat tacgtgccaa taagtatgac
      421 cttatcgtca atcttaccga tcaatggatg gttgctatac tggttcgctt attaaatgcc
      481 cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg taaaagtttc
      541 acccatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc cgtgctgaca
      601 ccattgggac ttgattcgtt ggtgaagcag acaaccatga gttacccgcc tgcaagctgg
      661 aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt ggttatccaa
      721 cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc tgtgattgat
      781 gccttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa agacgatctg
      841 gcctgcgtca atgaaattgc gcagggatgc cagacgccac cagtaacggc gctggctgga
      901 aaggtgacct tcccggaact tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt
      961 gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc gctgtttggt
     1021 gcgacagacc atattttctg gcgtccctgg tcaaataaca tgattcaatt ctgggcggga
     1081 gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt
     1141 attccggcgg cagatgtcat tgctgctgtc gataaattac tgccctcctc cacgacaggt
     1201 acgtcgttat gatcgttgct ttttgtttat ataaatattt tccctttggc ggtttgcagc
     1261 gcgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt cgggtttata
     1321 cccagtcgtg ggaaggcgaa tgccctgatg tatttgaact gatcaaagtg ccggttaaat
     1381 cgcataccaa tcacgggcgc aatgcggagt attttgcctg ggtgcaaaaa catttacgcg
     1441 aacatcccgt cgataaagtc gttggattca acaaaatgcc ggggctggac gtttattatg
     1501 ccgctgatgt ttgttatgcc gagaaagtag cgcaggaaaa aggctttttc tatcgcctga
     1561 cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag ggcaagccga
     1621 cacagctgct aatgctgaca gataagcaaa tcgccgattt ccagaaacat tatcagactg
     1681 aagcggagcg ttttcatatt ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc
     1741 agccagccaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa caacaatatt
     1801 tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac
     1861 ttgcttcgtt accggattcg ctgcgccaca acacattgct atatgttgtt gggcaggata
     1921 aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gcgcagtaat gttcacttct
     1981 tctcggggcg caacgatgtc tcggaattaa tggcggcggc ggatttatta ctgcatcctg
     2041 cctaccagga agcggcggga attgtgctgc tggaagcgat tactgcagga ttaccggtac
     2101 taacaacagc cgtttgtggc tatgcgcatt atattgtcga ggccaattgc ggcgaggcta
     2161 ttgttgagcc attccgccag gaaacattga atgagatttt acgcaaagcg ttaacgcaat
     2221 cttcattgcg ccaggcctgg gcggaaaatg cgcgacatta tgctgataca caagatttat
     2281 acagtctgcc agagaaagcg gcggatatca taacgggtgg tctggatggt tgaacttaaa
     2341 gagccgtttg ccacgttatg gcgcggtaaa gatccttttg aggaagttaa aaccttgcag
     2401 ggtgaggtat ttcgtgaact ggaaactcgc cgtactctgc gctttgaaat ggcgggcaaa
     2461 agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa aaatttactc
     2521 tcattgcgga tgccagtatt aggcgcagac cgcgaatgga atgcgattca tcgactgcgg
     2581 gatgtcggcg ttgatactat gtatggggtg gcatttggcg aaaaaggcat gaatccgctg
     2641 accagaactt catttattat taccgaagat ctgacaccaa ccataagtct ggaagattac
     2701 tgtgctgact gggcgactaa ccctccagat gttcgcgtaa agcgtatgct tattaagcgt
     2761 gtcgcgacga tggtgcgcga tatgcatgct gcgggcatta accaccgtga ctgttatatc
     2821 tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggagttaaa aatttcggta
     2881 attgacctgc accgggcgca gcttcgcacg cgcgttccac gtcgttggcg cgataaagat
     2941 cttattgggc tttatttttc ttcgatgaat atcggcctga ctcagcggga tatctggcgg
     3001 tttatgaaag tgtattttgc cgccccgctt aaagacattc tcaagcagga acaaggactg
     3061 ctgtcgcaag cagaagcaaa agccacaaaa atcagggaaa gaacgattcg aaaatcgttg
     3121 taatttccgg agagaaatat gagtgcccac tattttaatc cacaagagat gatcaataag
     3181 acaatcatct tcgatgaaag gcctgcggcg tcagtggcat catcattcca tgttgcttat
     3241 ggcattgata aaaactttct ttttggttgt ggtgtttcaa tcacgtcagt tttgttacat
     3301 aacaacgacg tgagttttgt tttccacgtt tttattgatg atatccctga agccgatatc
     3361 cagcgtttag cccaattggc gaaaagctat cgtacctgta tccagatcca tctggtaaat
     3421 tgtgaacggc ttaaggcatt accgacgacc aaaaattggt ctattgccat gtatttccgt
     3481 tttgtaattg cagattactt tattgatcaa caagataaga tcctttacct ggatgctgat
     3541 atcgcctgtc agggaaactt aaagccgctg ataacaatgg atcttgccaa taacgttgct
     3601 gctgttgtta ctgaacgcga tgctaactgg tggtcgttac ggggtcaaag tctgcagtgt
     3661 aatgaacttg aaaagggtta ctttaattca ggtgtcctgt taattaatac actagcgtgg
     3721 gcgcaggagt ccgtttctgc taaagcgatg tcgatgcttg ctgataaagc catcgtttcc
     3781 cgtttaacct atatggatca a
//
LOCUS       DAZUGK010000302         3760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZUGK010000302 DAZUGK010000000
VERSION     DAZUGK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.304"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..828)
                     /locus_tag="ACHY5W_004535"
                     /pseudo
     CDS             complement(77..828)
                     /locus_tag="ACHY5W_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            882..1307
                     /locus_tag="ACHY5W_004536"
     CDS             882..1307
                     /locus_tag="ACHY5W_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449279.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1304..1654
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004537"
     CDS             1304..1654
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449280.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            1685..3298
                     /locus_tag="ACHY5W_004538"
     CDS             1685..3298
                     /locus_tag="ACHY5W_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFS4449281.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(3329..3760)
                     /locus_tag="ACHY5W_004539"
                     /pseudo
     CDS             complement(3329..3760)
                     /locus_tag="ACHY5W_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          900 a         1006 c         1026 g          828 t
ORIGIN      
        1 atttcctgtc gtcaggcgtt caactcccga ctgcgggtta tggcccgctg cacgaacacc
       61 tgatggcagg tatcactcac ccggagaagg taaatccctc aaggggcagc aactcttcca
      121 gtcggtcttc cggccactcc ggtagacgcg tcagaacgtc tgtcagccag gcatgcggct
      181 ccagaccatt acgttttgcc gtttccagaa ggctcattat ctgtgccgcg cggttcccgg
      241 ccatcagtga accggcgaat aaccatgctt tccgccccat gaccaccggg cggatagcac
      301 gctcgcagat attgttgtcc aggggcacgg ctccgtcact caggaagcag ctcagttcat
      361 cgcgccggtt caggatataa ttaatcgctt tgtttagtgg tccgtcgggc ggacagcacg
      421 gctcctgctc ttcaggccag gcaaacaggt cattcactat cggccgggaa taccgttgtc
      481 gccattgccg tattttttcc acaggacgtt cacggatgaa cttctcaatg cgataaagac
      541 ccgcgatttt tctcagcgcc atggctgcac ggggctccct gctggcctta tacagatttg
      601 cgaaaccgcg ccttgcgtgg ctccagcaac aggcagatgt aataccgggg tcattatccg
      661 ccagagattt gtacacgctg tatccgtcac tcaccagcgt accgccacac cagccctgca
      721 gccaggcctc aggatatctg agggcccgtc cggtctgcga gtcaaaacag acgaccggcg
      781 gaccgcttct ttctcgtaag cgtaaactga ccgccgtatg tagccattaa gccagtattg
      841 gtaacgtagg tgcccacctt ttcaactagt ggacacctgt tatggaacag aaaatattat
      901 cctcagaacc ccgcagatca ttttcaaatg agtttaaact tcaaatggtt aaactggctt
      961 cacaaccagg agcctctgtt gcccgtattg cccgggaaca cgatatcaat gataacctcc
     1021 tgttcaaatg gctcaggctc tggcagaacg aaaggcgtat atcgcggcgt cttccggtaa
     1081 caacctcttc tggcgctggc gttgaattat tacctgttga gataacgcct gatgagcaga
     1141 aagagcctat ggcggctctt actccgcttt tatccactcc ttcacaaagc acagtcagtg
     1201 ccagttcctg taaagttgag ttccgccacg gtaacatgac gctggaaaat ccttcaccag
     1261 agctgctcac ggtgttgatc cgtgaactga ccgggagggg acgatgatct cgctcccatc
     1321 aggcactcga atctggctgg ttgccggcgt tactgatatg cgtaaatcct tcaacggtct
     1381 gggagaacag gtacaacatg tgctggatga gaaccccttc tccggtcacc tgtttatctt
     1441 tcgcggccga cggggtgaca cgattaaaat tctgtgggct gatgctgatg gtctgtgcct
     1501 gttcaccaaa cgtctggagg aaggccagtt tatctggcct gcggtgcgtg acggtaaggt
     1561 atccattacc cgctcgcaac tggcaatgct cctcgataaa ctggactggc gtcagcccaa
     1621 aacatcccgc cttaatgcac tgacaatgtt gtaaaaatgt catggccgga ttataaaaac
     1681 ggtcatgagt cggaaatacc tcattcgtat cactgagctg gaaaggctgc tctctgagca
     1741 ggctgaagcc ctccgtcaga aagatcagca actgagtctg gttgaagaga cggaggcctt
     1801 cctgcgctct gcactggcac gtgccgagga aaagatcgaa gaggaggagc gggaaataga
     1861 acatctgagg gcccagatag aaaaactgcg ccggatgttg ttcggtaccc gttctgaaaa
     1921 actgcagcgt gaggttgaac aggctgaggc ccaactgaaa caacgcgaac aggaaagcga
     1981 tcgttacagt gggcgtgagg atgacccgca ggttccccgc cagttgcgac agtcacgtca
     2041 tcgtcgtccg ttaccggcac atcttccccg tgaaatacac cgtctggagc ctgaagaaag
     2101 ttgttgcccg gagtgtggca gtgagctgga ttatctgggg gaagtcagtg ctgagcagct
     2161 ggaactggtg agtagcgccc tgaaagtgat ccgcactgtt cgggtaaaaa aagcctgtac
     2221 aaaatgtgac tgtatagttg aagcgccagc gccgtcccgc ccgatagagc gcggcatcgc
     2281 gggctccgga ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta
     2341 tcgtcagagt gaaatctttg cccgccaggg catcgaactg agccgggcat tactctccaa
     2401 ctgggttgac gcatgttgtc agttaatgac tctgctgaat gataccctgt accgttacgt
     2461 gatgaacacc cgcaaggttc acactgacga cacaccagta aaagtgctgg caccgggcag
     2521 aaaaaaggca aaaacaggac gcatctggac gtatgtccgg gatgaccgga atgcgggctc
     2581 atcagagcca ccggcggtct ggttcgccta ctcaccagac aggcagggaa aacatccggt
     2641 acaacacctt cgtcccttcc ggggtatcct gcaggcggat gcgttcagcg gttacgatcg
     2701 gctgttcagt gcagaacgtg aaggtggtgc actgacagaa gttgcgtgct gggcccatgc
     2761 ccggcgaaaa atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct
     2821 gaagcgaatc agtgaactgt acgccatcga ggatgaaata cggggattac cggagtcaga
     2881 gcgtcttgcc gtcaggcagc agcgaagcaa agtgttactg acgtcactgc atgaatggat
     2941 ggtggagaag aatggtacgc tgtcgaaaaa atccagactg ggcgaagcgt gcagctatgt
     3001 actgaatcag tgggatgccc tctgttatta cagtgatgac ggtctggcgg aggcggataa
     3061 taatgctgcg gaaagagcgc ttcgtgcagt ctgtctcgga aagaaaaact ttatgttctt
     3121 tggcagcgat cacggcggcg agcgtggagc actgttgtac gggctgatcg gcacctgccg
     3181 tctgaacggt atcgatccgg aagcgtatct gcgccatatc ctgagcgtac tgccagaatg
     3241 gccttccaac cgagttgatg aactcctgcc atggaacgta gtactcacca ataaataagc
     3301 gtcaatacgg tgctccgttg acgcttactc tttctccgct gacgtaagcc cacagccagc
     3361 cggagcagga tttaccgcct ttccgggtat caaggagcct cagggacgtt tcatctgcat
     3421 gaataacatc ccgtgtcaga agctcgtggc gtaacaattt tgccagcggg gccagtgcag
     3481 cgccagcaac accgaccata tccgccattg tactgacggg gagctccacc cccattcggg
     3541 agaaaatgtg ctgctggcga tacaggggca gatggtcggt atatttgctg atgacaacct
     3601 gtgcgatgac cgatggctca acagcacttt tcggaaggat atgtgccggc atttttccgc
     3661 tgaacacttt ctcacagcag ggacagctgt actgcgggcg gatatagcgg ttgaccacga
     3721 cctgtgccgg aatataatcc agcttctcgc tgacttcatc
//
LOCUS       DAZUGK010000303         3677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZUGK010000303 DAZUGK010000000
VERSION     DAZUGK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.305"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1431)
                     /locus_tag="ACHY5W_004540"
     CDS             complement(<1..1431)
                     /locus_tag="ACHY5W_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4449282.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAAL"
     gene            complement(1483..1692)
                     /locus_tag="ACHY5W_004541"
     CDS             complement(1483..1692)
                     /locus_tag="ACHY5W_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFS4449283.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(1788..2162)
                     /locus_tag="ACHY5W_004542"
     CDS             complement(1788..2162)
                     /locus_tag="ACHY5W_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS4449284.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2168..2884)
                     /locus_tag="ACHY5W_004543"
     CDS             complement(2168..2884)
                     /locus_tag="ACHY5W_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4449285.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCAIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNPEKTLTVN
                     TGALLTMSVSVNGGTPPYKYAWKKDGQPVEGQTTDTFSKANAQSGDKGAYTCMVMDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(2952..3296)
                     /locus_tag="ACHY5W_004544"
     CDS             complement(2952..3296)
                     /locus_tag="ACHY5W_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFS4449286.1"
                     /translation="MTEADLYPYLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3293..>3677)
                     /locus_tag="ACHY5W_004545"
     CDS             complement(3293..>3677)
                     /locus_tag="ACHY5W_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4449287.1"
                     /translation="SGAENNRVLREATRAGANVLKEEVVSRAPVRRGKLRRNVVVLSR
                     RSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFVRPAF
                     DVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          780 a         1222 c          885 g          790 t
ORIGIN      
        1 cagcgccgcc cgtgttcgtc cttcattatc tgaggcttca ggcaaagaca ttgacggcgt
       61 tttatgcgcg atttcatcta tcgtcagtgc atactggcgc gcagactccc tggcctgctc
      121 ctgattctgg tacagcgtgt accatgctgc agcccccagc atcaccagtc cgggtacgcc
      181 accaaccagc cccagcgcac cgctcatcag acgtgagccc accgccgttg tactgttcag
      241 cgcattctgg gcggcgcttc tggcagcaat atttctgttc aggcgttcct gtgtggccgc
      301 cagacgggcc tctgcagcaa tctgcatctc cgtcccgcgg gctgccgcca cggcctgctg
      361 agcacggtac acggctgccc ttgcccgcgc cgtggcaatc tgcgttcccc tgaactgtgc
      421 ttccgccagt gcaacttcat tacgtgcagc cgtcacaagt cctgccgtgg cagacatcgc
      481 tccggaggcc atattgccaa agtaccgggc aaccccgacg gcaaccagcg cgcccacggc
      541 tgttgccaca ttatcaatct gtccggcaac accgttcagc atgccggaga gcgtttttgt
      601 caccccgctg gcctcattcg caccgcccac ccaggccata aaggcgtttt ccaccttcgt
      661 gatactactg gaaaccgttt ccggcatggc cgcatattca tcacgtaata tccccagttg
      721 gctgattaac gcggggacca ctttatccgc tgtcagtttt ccgtcatccg ccattgcctt
      781 cagatcttta cgggccacgc ccatacccgc agccagtgca cgtacgatcc ggtcaccact
      841 ttcattgacc gaattaaact cctcaccacg caatacaccc tgcgccagcg cctgactgaa
      901 ctgggtgatc accgagcccg cctctgccgt actggcaccg gagattttca gccctgtcga
      961 aatggcctcc gtcaccttca gcacatcatc agcactgtaa ccatattcac gcatcgaggc
     1021 agccgaacgg gcaaacaggg ccgcattatc cgaaaatgcg gtgcctgtcc gctggctgat
     1081 atccatcagc actttctgtg atgacgcaaa ttcatccgat gactgcgacg cctgtttcag
     1141 acgggcattc acggagctcc attcatccgc cagcgaaatc aggtgtccgg tggcaaaggc
     1201 accggcaaac gcaccggtca ttccgacagc cgaagcgcgg atttccgtca actggctgtg
     1261 cagctcagcc agagcccggc gctgctccct ggctgccgca gcagcctgac gtccgccatt
     1321 ctgcagggtc cggtaatatt cactgcccat acgggacgcc cgctggatct ccgactggaa
     1381 tgactgtgaa tttgccgaaa ttttgataat cagttcacgt aacgtcgcca ttcacctttc
     1441 tccgggcaaa aaaaacctgc cacagcaggt tttcatcatt atttatgaca ttgctgcaag
     1501 gctcagcgcg tcttccagcg ccgcaaacgg atccacctcc ggcttatcct catcctcgcc
     1561 ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga aaaccgcttt
     1621 cacgatctgc gcattacgga tatccccgcg ctcatcaccc agcggggata ccctgtcgaa
     1681 ctccatccac atcatcgcct cgctcgcact caggctgtgc cgcagttcgg ataaggtgcg
     1741 ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta cttttttctg
     1801 gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc atgtaccgga
     1861 ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgttg caggtcagta
     1921 tcacacagga catcgcagaa cagcgtcaca tccgcttcca ggttacggcg ggttttcgcc
     1981 accaccgaca gggtatcgtc atcctctcca tcaccattga gcacttcctg ccacagatac
     2041 caggcctctg ccgaaggctc ccgcagcacc acgctgacat ttctccattc cggcaccttc
     2101 accgttttat gacggaaccc cgacagtctg gccagcgcca gtgttttcag atcttttgcc
     2161 ataagcctta tccgcccgca ccattaaccg ttaccgtaca cgcatcagag gtaatgctct
     2221 gcggctgttc tgcagaatcc attaccatgc aggtataagc ccccttatca cctgactgcg
     2281 cattggcttt actgaaagtg tcagtagtct gtccctcgac cggctgacca tccttcttcc
     2341 aggcgtattt ataaggcggc gttcccccgt tgacactgac tgacattgtc agcagcgcac
     2401 cggtattcac ggtaagtgtc ttctccggat ttttcacaaa cgccagcggt accacatagg
     2461 acaccggttt acccttcagg cgaagtgaaa acgttgcagc caccacgccg ttggtaccgg
     2521 atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg gacggaaact
     2581 gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg ttctgggcct
     2641 gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga ccgttgatgt
     2701 tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctgc ccggcggtga
     2761 actggacttc cttgatggcg cagtccaggc gcagatattc cgccttatcc atagtttcag
     2821 cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg tgagcgttca tacaaagcag
     2881 acattctgcc tcctgataat aaaaaacccg cacgcggcgg ggtatgggtt ttgtagaaaa
     2941 aaaagaaaaa gtcacaccgt gacctgaaac tccagggttg cacggtaaca gcggttttcc
     3001 ggaatatagt cctgcatttc actgacggat cccggggcca gcagcattat ggcttcacgg
     3061 gcgtcctgac gtatctgacg cgcctgcgtc acagtcccgg cataaacgtc tatctgcacc
     3121 gacactgagg actccgcctg cccgcccatc acgtccgccg acaccgatga aatcaggctg
     3181 aaaaccaccc acggaagcgc caccgacggc ctgccatcca gcagggggac cacatacggg
     3241 tacacctgcc cgccggcaag atgcgccaga taaggataca aatccgcctc cgtcatcgtc
     3301 tcagtacctc atcaatggcc cggttcatcc gcgcaatcgc cacctgagct gcctgttcac
     3361 tgcgcacatc aaacgccggg cgcacaaacg ggtgcggtgg catattcacg gtccccattt
     3421 ccacaaaccg ccagtagaaa gcattgcgcg ggttatccgc cttcatggtg ttatcgctgt
     3481 taccggtgtc cggattaaca ccacggatat ggacaccgga ttccatcccg ccatcgcggg
     3541 agcgccggga aaggaccacc acattgcggc gcagttttcc cctgcgtacc ggtgcccgtg
     3601 acaccacttc ttctttcagc acattcgcac ccgcacgggt tgcctcacgc agcacccggt
     3661 tattttccgc accactc
//
LOCUS       DAZUGK010000304         3566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZUGK010000304 DAZUGK010000000
VERSION     DAZUGK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.306"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..481
                     /locus_tag="ACHY5W_004546"
     CDS             <1..481
                     /locus_tag="ACHY5W_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449288.1"
                     /translation="AGAPAPDILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDGKP
                     VVPGMKLSKEKCDRVNAIERDKALAWVAKNIKVPLTEPQKAGIASFCPYNIGPGKCFP
                     STFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            755..1450
                     /locus_tag="ACHY5W_004547"
     CDS             755..1450
                     /locus_tag="ACHY5W_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFS4449289.1"
                     /translation="MKEMISIDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1545..1676
                     /locus_tag="ACHY5W_004548"
     CDS             1545..1676
                     /locus_tag="ACHY5W_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449290.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            1899..2084
                     /locus_tag="ACHY5W_004549"
     CDS             1899..2084
                     /locus_tag="ACHY5W_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4449291.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2485..2811
                     /locus_tag="ACHY5W_004550"
     CDS             2485..2811
                     /locus_tag="ACHY5W_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFS4449292.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(2943..3143)
                     /locus_tag="ACHY5W_004551"
     CDS             complement(2943..3143)
                     /locus_tag="ACHY5W_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4449293.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3185..3550
                     /locus_tag="ACHY5W_004552"
     CDS             3185..3550
                     /locus_tag="ACHY5W_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4449294.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          884 a          770 c          997 g          915 t
ORIGIN      
        1 cgcaggtgcg cctgcgcctg acattctcga ccagtttctg gatgaaaagg aaggtaacca
       61 caccacggca taccgtgatg gtgcgggtat ctggaccatc tgccgcggtg ccatcctggt
      121 ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag gaaaaatgcg accgggttaa
      181 cgccattgag cgtgataagg cgctggcatg ggtggcgaaa aacatcaaag tgccactgac
      241 tgaaccccag aaagcgggta ttgcgtcatt ctgtccttac aacattggcc caggtaagtg
      301 tttcccgtcg acgttttata aacgaattaa tgcaggagat cgaaaaggtg cctgcgaagc
      361 gattcgctgg tggattaagg acggtggcag agactgccgt attcgctcaa ataactgtta
      421 tggtcaggta tcccgtcgtg accaggagag cgctctggcg tgctggggaa tcgacagata
      481 agcagaatat tttgctgaaa aatgacgttg gtcagcgcgg gcggataaca cgaaatcctg
      541 cgaactggca aaatgtaagt gaataaagtt aggcagatta tttcacgcag aggcaccgta
      601 atggtgcctt tgtcatttct gcgcttcgca caagcgtaaa taaaccaaag aacctttcag
      661 gatgagccct ggtggataac cggcagtggt ctggttaacc ctctttgggc tggttattcc
      721 tgtgcgcagg gttcatcact aaaaggaatc aaccatgaaa gagatgattt ctatcgatca
      781 tgaaatatcc atgagtagtc tggattttct gaataacatt attaatccag cccgggcaga
      841 agccggagaa gtccctcatg aaccgcgtaa gtttcttgca aaaattgagg atgagctaga
      901 gcttgatgga accggaaaaa aattccggtt aaacaataac cagacaagaa cggcatacta
      961 tgatctggat tttgaccaga tgatgctcgt tggcatgagg gagtcaaagg ccgttcgtcg
     1021 ttctgtgctg gcaagactca aagcgatgca tggtattcag atcccccgga ctttacctga
     1081 ggcgttgcga tttgcggcaa aactggctga acagaaagca gtgctggaaa atcaactggc
     1141 aatagcagcg ccgaaggctg aatttgttga taactatgtt gaagcatctg gtctgatggg
     1201 attccgggaa gttgctaagt tactcggtat caaagaaacc gatttccggc tgtttttgtt
     1261 ggagaacgga ataatgtatc gcctcgctgg aaaaatgacg ccttactcgc atcacctgga
     1321 tgcggggcgg tttagcgtga aaacgggcga ggcgggcaat ggtcatgctt ttacgcaggt
     1381 taaattcacc ccaaaaggtg ttcagtggat tgctggtctg cttgctgcat ggagagctac
     1441 cgcagcatga agatgataaa aacggactgg aaatttttgc tggtctggct gattccgttt
     1501 ttatgggtgg ttgcccggtt aattactgct attaaggggt aaagatgtca gacaaactca
     1561 taatgctggc gaagggcctt tgtgtaatcg tcggtatttc attttcacta atgctggttg
     1621 ttctttttct ttccataacc tggatggcgt tgacttcggc agggctggtg gggtgagcat
     1681 aaaccgaatg ctttccgcgt ttaccgttat tctgctggtg gtctgtggtg cgcttagtct
     1741 ggggctgaat cattaccgcg ataacgtcat cacctacaaa gcgcagcgcg ataaaaaagc
     1801 cagagagctg gagctggcaa acgcaaccat tactgatatg caggtgcgcc agcgcgatgt
     1861 tgctgcgctc gatgcaaaat actcgaggga attagccgat gcgagagctg aaaatgaaac
     1921 tctgcgtgct gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg
     1981 taccgtgcgt gaagccaccg gcacctccgg cgtggataat gcaaccggcc cccgactggc
     2041 agacaccgct gaacgggatt atttcatcct cagagaacgg ttgatgacaa tgcagaagca
     2101 gctggaaggg gcacaggact atatccgcac tcagtgcctg aactaagttt tgctgatgcg
     2161 ccgtatcgtc gctgtattcc ctcattaaca gagaccgcag cccgacaggg agactcctct
     2221 gcgcgagtgt gcggggataa tcaaaaacga tacacaccgg ggtttaccgc gttaacggag
     2281 cgcggcgttg tcccctcatg gtcgctggtt cggtgcgatg gtggaagaaa ccggacgatg
     2341 tgttacctcg caagacctgt tatgtcatgt gtctgatttg tgatttaagt cggataattg
     2401 tcgttgccat taagcagagg attgatggcc gacagggtgg cattgttaga ataagactta
     2461 ttcttatctg tgccgggaat gaaaatgaaa agaaatcttc cgttaattat tttgttgtct
     2521 tctctggtta tgggctgtac gcaacataaa acagatatgc cccgacagtt ggttaaggca
     2581 ttaccacaat atccggccta tgcagcggca aattatataa agggacgggt tgatgtgaag
     2641 tttgatattg gtgctgatgg tactgtcacc cgaattgagt ttatccgttc agagccgcac
     2701 catctgtttg atgagcaggt tgtaaaggcg atggcaaaat ggcgatttga gaaggacagg
     2761 ccgcgtaaag gcgtgaagaa aacgtttatc tttagtcctt ctgcaccctg attatttcat
     2821 cagaaattaa ttatcactct gttgttattc tgtacattcc ggctgggtaa gtcttgttcc
     2881 gccgggtatg aagatgaaat attgttggag gacagtgggt acctgctcct gtaaccgaac
     2941 gttcatttct cgttatttgt catgctggcc ggacgcagat gcgttgcatc tgttgccagc
     3001 cttctcctgc aggcttcaat aacccacgct gaaaagttac cggaaccttt atgttcaagg
     3061 gcgatattga tctgttcaat catgtgattg gggaaacgga tattgcgggt tgtggttctg
     3121 cgggtccggt ttttcgatga catatttatt tcctttactg attgccatat gacggggatt
     3181 ttacatggct gagcttcgta cactccagag cagaatcaaa acactgaata cccgacgggt
     3241 gaatattctg aagggtgaac agcgtcgtgt cagtggcagt gcacgtgttt ccctcaagcg
     3301 tcatatctgg ctgagggatg ccggacagtg ctgtctctgt ggtcgtgtgg ttgacctctg
     3361 tgacagtgaa ctcgatcacc gaattgcact tcagttcggt ggtggtaatg aggagacgaa
     3421 tctctggacg ctctgtaccg aatgccatcg acaaaagtct gctcgtgaag cggcgggtgg
     3481 tatgccggac ccgacgctgc cggaggtgtc cggaggtagt ggcagagcgg acgacatcat
     3541 cggactgtaa cccgagcggg ggggta
//
LOCUS       DAZUGK010000305         3473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZUGK010000305 DAZUGK010000000
VERSION     DAZUGK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.307"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..852)
                     /locus_tag="ACHY5W_004553"
     CDS             complement(70..852)
                     /locus_tag="ACHY5W_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4449295.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(854..1267)
                     /locus_tag="ACHY5W_004554"
     CDS             complement(854..1267)
                     /locus_tag="ACHY5W_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449296.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1827..2057
                     /gene="vapB"
                     /locus_tag="ACHY5W_004555"
     CDS             1827..2057
                     /gene="vapB"
                     /locus_tag="ACHY5W_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFS4449297.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2054..2470
                     /locus_tag="ACHY5W_004556"
     CDS             2054..2470
                     /locus_tag="ACHY5W_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFS4449298.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2632..3319)
                     /locus_tag="ACHY5W_004557"
                     /pseudo
     CDS             complement(2632..3319)
                     /locus_tag="ACHY5W_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          844 a          915 c          919 g          795 t
ORIGIN      
        1 atacaagaga tcacatattg cagtggtctc ttttttatag aagaaatcac aattcataat
       61 tgtgacttat tatgcatgtt tgtttttcag cattgtgacc gcatcggact caggcatcga
      121 aaactgcacc cggtgccggg cagcgacatc cagcgcaaac accttcgtgt agacctccgt
      181 tgagctgatg gacttatgcc ccatcagact ctgcaggacc ttcagcggta taccggcata
      241 cagcatatgc atcgcatagg aatggcggaa cgtatgtggc gtgaccggga cagagaacgt
      301 cacaccatca gcggcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
      361 cgtcacttcc cagatccgcg ctttctctgt cctgcctgtt cgtttattac gccgttccat
      421 ggggattttc aatgtggcca ccatcgtctg cagctggctg acgtaccagg agtcggagag
      481 cggaaccagc cggtgagtct gctgcccggc gggcgttctt cctgccgtcc tggcggcttt
      541 ttcggtccgt tgtttcaggg tcgcaagctg cacaaacgga tacggaggcg tgagcgaaaa
      601 atccccccgc gtcagcgcca gtgcttcatt aatgcgcgca ccggtgttcc acagtgtggc
      661 cagcagcatc ttgcggcgca gatccgggac gtaatggagc agggcgctca cttcaggggc
      721 cagcaggtat ttgggcagtt catcatgaac catcgacatc tggcggagtg ccagcgctgc
      781 cggataatcg atggcaacag gaagctgagc ggcatcccgg gcatggtgga tcggtgccgg
      841 caggtgctgc atatcagtgt tcactccacg gggaaacgtc gggcaggttt tccacaaccc
      901 ggtactttcc cggataacgg agctggttac cgccacgcgt ccagacggtg acaacaaaac
      961 cccctccctg gttgttttcg gggagcaggc gatcgcactg ctcactcagc atgtggatca
     1021 cgaacggcgc atcgctgccg tcttcaaata tcagttccag cgcatcgcgg ccatgcagcg
     1081 ttcccttact gatgatgacg tactcagccc cccgcatctc cctgagtaaa tgttttgccg
     1141 catccgggac aagcagcctg gctgcgccgg cattccagct gaggtagaca tatccggcct
     1201 gcgcctgtac agactgccag taatccgttc tggcgacagc ctgaccttca ttctcaataa
     1261 aaaacatggg tgactccttt tttcttttcc gggcagggac ggtcatgcga taacgtcatc
     1321 catcatcact gctcttaccg tcacgccatg tgataacatc atgccgttat catcatcacc
     1381 atgataacaa gcatccatat aaccacgctg ttaaattacc ggaggaccat ttccgacaga
     1441 tccgattttt ctgttattga cgggttttcg gaagtgtatc acgctgagga aataatcata
     1501 tcgctgtttt ccgcccctgc gcgacgcatc acaggtcagg gagatgacac aacatatagt
     1561 gtcatcagca gatgaatccg gcaccacatg ttgtgatgac gggaggtggc gttgtgtggg
     1621 gggagaggaa gatcctgaac aacctttttt cggatctccg ataaaaaaga aagaaataga
     1681 gatatgctaa acatatgcct ggcatctaca tatgctaccg ctatacatat gccacacatc
     1741 tgcctatact aaacatatgc acacacccgg tgctttgaat attccggata tgtttcacat
     1801 atccatatgc tcgggagaga aagaacatga gaaccgtatc gatatttaaa aatggcaaca
     1861 accgcgccat ccgtctgcct cgtgatctgg attttgaggg ggtgagcgag ctcgagatcg
     1921 tccgggaagg ggacagcatc atcctgcgcc ccgtccggcc aacctgggga tcattcctag
     1981 agtacgaaaa agccgatcca gattttatgg cggagcgcga ggatgttgtc agcgacgaag
     2041 ggcgatttaa cctgtgaaca agacttatat gcttgatacc tgtatctgct cgttcatcat
     2101 gcgtgaacag ccagaagccg tcctgaagcg tctggagcag gcggtgctac gcggccaccg
     2161 catagtggtc tcggccatca cctactctga aatgcgcttc ggggctaccg gcccaaaggc
     2221 ctcaccacgt cacgtccaac tggtcgatga attctgcgcc cgcctcgatg ccatcctgcc
     2281 ctgggatcgc gccgcagtag acgccaccac gaagattaaa gtggcactgc ggctggccgg
     2341 gacgccgatc ggcccgaacg acacggcgat tgccgggcac gccatcgccg ccggggcgat
     2401 actggtaacg aataatacaa gagaatttga gcgggtgcct gacctggtgc tggaagactg
     2461 ggtgaagtaa gacactcttt tcaaagtcag gagataaggg caccaagaaa cagcccgtat
     2521 acgggctgtt ttaacagata tctacagtaa gcggtcctaa actgtagggt atcttcgaag
     2581 atcagactat cagggagcta aagcctatct tccggcggca aaaacagtgg ctcaagcgat
     2641 acgggcaacc gagctctggt ttgctgccgg tggcagggcg atgagcttgt cacattccat
     2701 aatcacgtac tccgggatga gatcgaatct ggacggatct agttgacaga tgaactcatt
     2761 ttcaatgtgg tacgtttcgt taacgaattt tcgtattacc ggatggtcct gatccagttc
     2821 cagtaaacgg aatacctgca gtttttcata gccgttacga cagcgacggt acaggttttt
     2881 tatttcatct gggctgctga cgagagcctg aagtcgcggg taatcaaaac tgtccacata
     2941 ctcccggata tcccgcaagg cctgctgaat tttctcagga gccattttgg ggtagcccga
     3001 gcccgcagcg gcaggctcgc gataatccag cggggcgacg cgtcgatgga atagattgga
     3061 tagaagctga taagcatcac cacgttcgtc aacgatttca aagtagcgac gaagatagat
     3121 gagcttaatg atctcctcac agtctgctga cgcagtgatg gatttgcaga tctgcatgaa
     3181 tgatatcgcc gtcgttaaca ggtaattctt ctatgactcc agcagacagg cgcaggcagg
     3241 aggcggtcac ctgattactg aacaaccttc tgactgactt cagcgtgtca atcacaggct
     3301 ccacgtcatg cgtcagcata gtgctgatgc tgacggatgt tacaaaaaat gcagcgtatg
     3361 gcgttgagat agaaagtctg gtgctggaga taaatacacc tacatcatga taaaaaaggg
     3421 ccagcgactg acctgaagaa gacactggct aaaaggcttt gcatgtttgt aga
//
LOCUS       DAZUGK010000306         3394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZUGK010000306 DAZUGK010000000
VERSION     DAZUGK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.308"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACHY5W_004558"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACHY5W_004558"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4449299.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACHY5W_004559"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACHY5W_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFS4449300.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHY5W_004560"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHY5W_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS4449301.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2479..2658
                     /locus_tag="ACHY5W_004561"
     CDS             2479..2658
                     /locus_tag="ACHY5W_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFS4449302.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHY5W_004562"
     CDS             complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHY5W_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFS4449303.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          926 a          786 c          806 g          876 t
ORIGIN      
        1 attatcgcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg cgccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cactgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat
     1561 tattcacggt gatgcccttg ccgaacttaa aaagctacct actgaaagcg tcgatctgat
     1621 ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca tcatgaacag cacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt
     1861 gcaggcgaaa aaacactacg gttccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acgttcaacg gtgatgctat tctggtagaa gccaaaactg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccttac aatcatcaaa aagtaccggg
     2041 taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaga accacccgac
     2101 gcaaaaaccg gaagccttac tgaaacgcat tattcttgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccgtct ccagcgggcg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa
     2341 cctgtcaaaa cgaagtcggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa
     2401 gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac agaaacagac
     2581 aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaatgtgaat ttacatgaca cttaattcat tcgtttgatg aattaatttc
     2701 gttatgtttt catctggcat gaacattctt aatacgttat cgaccagagc gggggcttgt
     2761 ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca
     2821 ccatcaataa taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc
     2881 tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc
     2941 actccctcgg ccagcatctc atcattaaat tcacatttgt gatataagat tttcagcaac
     3001 gcctgctggc ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat
     3061 tgttgaaacg ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat
     3121 gaaggctgtt gcaaccacat ctcattaaac agttgagtct tgttttcgaa gtgccagtag
     3181 atagcgccac gcgtaacgtt agcggcgtcg gcaatgtcgt tgagcgtcgt cttgcttacg
     3241 ccatgctgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct
     3301 tcggctttgg ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacaga
     3361 gtgagaaaat agcgaaggtt aatctatcac ctaa
//
LOCUS       DAZUGK010000307         3381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZUGK010000307 DAZUGK010000000
VERSION     DAZUGK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.309"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..100)
                     /locus_tag="ACHY5W_004563"
     CDS             complement(<1..100)
                     /locus_tag="ACHY5W_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449304.1"
                     /translation="MTSREARLGMFLASLLRRIAFSYYDYKAYNFNI"
     gene            388..1704
                     /gene="shiA"
                     /locus_tag="ACHY5W_004564"
     CDS             388..1704
                     /gene="shiA"
                     /locus_tag="ACHY5W_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFS4449305.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1806..3260
                     /gene="amn"
                     /locus_tag="ACHY5W_004565"
     CDS             1806..3260
                     /gene="amn"
                     /locus_tag="ACHY5W_004565"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFS4449306.1"
                     /translation="MNNKGSGLTPAQAQDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          785 a          827 c          810 g          959 t
ORIGIN      
        1 caatattgaa attataagct ttataatcgt agtaactaaa tgcaatacgt ctcaacaatg
       61 atgctaaaaa catacctaac ctcgcctccc tactggttat aatgcaatgc agtctatcag
      121 actcatcagg gtgccatttt gtgcatatgc ggacttttat gtttcatatc tcaaacctgt
      181 gggtcctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat cttccagcga
      241 atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc tgtcgaggta
      301 gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc atactaatta
      361 taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac tcgtcccgat
      421 gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagttt cgctggtgcc
      481 gtcgtcgact ggtatgattt tttactctat ggcatcaccg ccgcactggt gtttaatcgc
      541 gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc tacctttggc
      601 gtcggattcc ttttccgtcc gctcggcggt gtcattttcg gtcactttgg cgaccgactg
      661 ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gtatcgcgac agccttgatt
      721 ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttact ggtgacgctg
      781 cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcggcgtt gctttccgtt
      841 gaaagtgcac caaaaaataa aaaagccttt tacagtagcg gtgtacaagt tggctacggt
      901 gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac gactgacgaa
      961 cagtttttaa gctggggctg gcgtattcct ttcctgttta gcatcgtact ggtactggga
     1021 gcattgtggg tgcgcaatgg catggaagag tccgcggaat ttgaacaaca gcaacataat
     1081 caagctgccg cgaaaaaacg catcccggtt atcgaagcgc tgttacgaca tcctggtgct
     1141 ttcctgaaga ttattgcgct acggctgtgc gagttgctga cgatgtacat cgttaccgcc
     1201 tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt ccttaatatt
     1261 ggtttgctgg taggtgggtt aagctgcctg acaattccct gttttgcctg gcttgccgat
     1321 cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa tcggaacgtt gagcgcattt
     1381 cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg
     1441 ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg
     1501 tttggtgcca gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt
     1561 ggtggtggat ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg
     1621 catagcgtcg ccatttattt gctggctgga tgcctgattt ccgcaataac cgctttgttg
     1681 atgaaagaca atcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac
     1741 ttttgtttca catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga
     1801 cacacatgaa taataagggc tccggtctga ccccagctca ggcacaggat aaactcgacg
     1861 cgctgtatga gcaatctgta gtcgctttac gcaacgccat tggcaactat attacaagtg
     1921 gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg
     1981 taacctggga cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc
     2041 acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg tatctcaatg
     2101 aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa ccctcgcagc
     2161 atgaaatccc ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga
     2221 gcgctgggtt aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg
     2281 cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat gcgcgccgcg
     2341 tcgatttttc cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc
     2401 cgttcgtctt gtttaccaac tatacacgtt atgtggatga attcgttcgt tggggatgca
     2461 gccagatcct cgatcctgat agtccctaca ttgccctttc atgtgctggc gggaactgga
     2521 tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc
     2581 agatgcccgc gtggcattta attaccgccg atggtcaggg tattactctg gtgaatattg
     2641 gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg
     2701 tctggttgat gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg
     2761 tacttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata
     2821 ttcctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga
     2881 gcggcaggcc cggtgaggaa gttaaacagc ggctacgtac tggcactgta gtaaccactg
     2941 atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg
     3001 ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc
     3061 catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt aaacttcccg
     3121 gccaggctaa ccgtttttat gaaggtgcta tttccgaaca cctgcaaatt ggcattcggg
     3181 cgatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta
     3241 atgagccgcc gttccgataa taagttctga accttcttat caaaaataga tgtgaaggat
     3301 taagtaagac atctggcaaa tagcccgcaa taaacgcggg ctctttgctt ccttgggcgg
     3361 atacgagttt tattatcgtc t
//
LOCUS       DAZUGK010000308         3363 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZUGK010000308 DAZUGK010000000
VERSION     DAZUGK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.310"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(611..808)
                     /locus_tag="ACHY5W_004566"
     CDS             complement(611..808)
                     /locus_tag="ACHY5W_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449307.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(874..1144)
                     /locus_tag="ACHY5W_004567"
                     /pseudo
     CDS             complement(874..1144)
                     /locus_tag="ACHY5W_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1449..1637
                     /locus_tag="ACHY5W_004568"
     CDS             1449..1637
                     /locus_tag="ACHY5W_004568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449308.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1965..2159
                     /locus_tag="ACHY5W_004569"
     CDS             1965..2159
                     /locus_tag="ACHY5W_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449309.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2502..2630
                     /locus_tag="ACHY5W_004570"
     CDS             2502..2630
                     /locus_tag="ACHY5W_004570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449310.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2940..3275)
                     /locus_tag="ACHY5W_004571"
     CDS             complement(2940..3275)
                     /locus_tag="ACHY5W_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449311.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          999 a          768 c          735 g          861 t
ORIGIN      
        1 attcaggata cagtttgtag gagctttctt ttttgaacaa tgatttgcag gacagttatg
       61 ctaaactagc ggaatcagta tcacaacaat aaatgactga tcatacctga aatttgaatt
      121 aacaggcatc atgaatgtca atagtctaag cggtgactac ttatatcgct tgcataaata
      181 ctaaacactg actagaaaag tattggataa ccccgtcact aaaagtaacg cttcggttat
      241 ttgctcggaa tgtattaacg ccaagattgt tagccgttac gccttatatc actggcgctg
      301 acaaatgttt cttttctaat ttactgaacc ggcgcagaca cgcaggaaag cacgccggca
      361 ccggtgtaat gccagttcta atcaataaga atggaatgac gaatcagtca ggaagggata
      421 acaacccgct gctgaacgga aggtatcagc aggaagatga acgggaatat cgccagcacc
      481 aagggcaggg ccgggttcca caataccagc gctggcattt tgcacattgc cagcgtaccg
      541 gcattacaac acgtttcatg ctgctgcccc ccaggattcc tcgattcgtt gctacatcca
      601 ctgctctact tcacttccac tgctccactt cacttttgta ccagcggctg ctgcgcccca
      661 gtttgatggg tttagggaaa tggccttcac tgatcaactt gtaaaaccat ttgtcggtca
      721 taccggtgaa actggtgata tagttcatgt cgatcaggga attatcggca cgcagaaata
      781 cgctgtcgtg gcttgttgta gtcaacatgg tcagaatgtc ctgtgagatt tatgaagatt
      841 gtgcgaatgc gggaaatcta ctgagctgtg ctttcagaac tggcctgtta cgggatacca
      901 gggattaccg gcggggtaac gggcttccgg atcatacaca ccacgattat cgcggacaaa
      961 atcactgaac gcccatatca cctctttaag tatgtcttcg cagtccggta aatgacgatc
     1021 cagcgccaca tcccgggtgg tacagctttg atgcgcccgg tgacacaaag cccggattgt
     1081 tccagacatc ctgaatcaaa cgccccagat taggggcgtc gaaatatgcc tctctgacca
     1141 ttatattccg gcgtataggt agcaggtctg aagtgacaat gcgtcacctg acgttaaaag
     1201 tcacccaaga tgaccccagt gggggcaaca agtataaaac cgttttttga ttaagtggtc
     1261 ggatatgaaa aggggaaggt ttatgtgaaa aatacctgtg ttttctttga aaacacacaa
     1321 cagtatcagg ttatttatgt ttatacagtg cgttccaaac tgacctgaat aattacgcaa
     1381 gttgcaacaa taaaacattt ttgccgtagt gtgaggtact ccggtaaatt gcttcccagg
     1441 tgcagacaat gactaacgaa gaactacagg caatcgcatt atccatgctt gagcgccagc
     1501 gcgaaaaagt acaccagcaa cgagagtctt tgcttaaccc atctccattc accaggccaa
     1561 aacgattgga gcttgaagca ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa
     1621 ccagaccacc aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac
     1681 catcagcgcg gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt
     1741 tatcaccaga agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca
     1801 ttatcgaaca gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat
     1861 ctcctttagt tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt
     1921 tttcattagc acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa
     1981 taaaaaggct ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac
     2041 gtgccgcgct gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata
     2101 atggcaaaaa aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac
     2161 aacaatctgc atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt
     2221 ctttatatcc actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta
     2281 caagcacact agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata
     2341 cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc
     2401 catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc
     2461 ccaatatcct gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag
     2521 agttctcggt acaggggaaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg
     2581 aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta
     2641 tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt
     2701 ggcaatatcg aattgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat
     2761 gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct
     2821 aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct
     2881 gcattatgtt aaataaatat aaaaagatgg tttaaatacc ccgttacttt tgacttacac
     2941 tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc
     3001 acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct
     3061 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
     3121 cttttaccag tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt
     3181 atagcattgt ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca
     3241 gtccgttttt cataacccac tgtactgata accataatct aatcagtaga aattgagtcg
     3301 aaaataagca ctactccata caggataatt agaggtcagt ttgattattc acaattcatc
     3361 atc
//
LOCUS       DAZUGK010000309         3350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZUGK010000309 DAZUGK010000000
VERSION     DAZUGK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.311"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..790)
                     /locus_tag="ACHY5W_004572"
     CDS             complement(<1..790)
                     /locus_tag="ACHY5W_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4449312.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWT"
     gene            complement(765..1310)
                     /locus_tag="ACHY5W_004573"
     CDS             complement(765..1310)
                     /locus_tag="ACHY5W_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4449313.1"
                     /translation="MKVNKKKLAEIFKVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            complement(1757..1981)
                     /locus_tag="ACHY5W_004574"
     CDS             complement(1757..1981)
                     /locus_tag="ACHY5W_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449314.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(2046..2252)
                     /locus_tag="ACHY5W_004575"
     CDS             complement(2046..2252)
                     /locus_tag="ACHY5W_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4449315.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRLH"
     gene            complement(2475..2606)
                     /locus_tag="ACHY5W_004576"
     CDS             complement(2475..2606)
                     /locus_tag="ACHY5W_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449316.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            complement(2619..2801)
                     /locus_tag="ACHY5W_004577"
     CDS             complement(2619..2801)
                     /locus_tag="ACHY5W_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449317.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRAQAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            complement(2958..>3350)
                     /locus_tag="ACHY5W_004578"
     CDS             complement(2958..>3350)
                     /locus_tag="ACHY5W_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449318.1"
                     /translation="IWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEK
                     NIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRD
                     CRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT          820 a          940 c          765 g          825 t
ORIGIN      
        1 gcgtccattt gaggccaaac ggcgtctctt tgtcgccaaa tttaagatac tgctcctccc
       61 cgcagtgcgg gcaggcaaca tgaaaacgca taaaatgcgg ggattcactg gctgcacgct
      121 caatctggca ggtgcctctc actttgggcg tggagccacg gatggacttt ggccagaccg
      181 agccttcaat acgcttgtca cccaggaacg tcggagagcc ttcctgttca atatcctcat
      241 caaaggcagc aagttcatca taacccgcca catcaaccga cttttcacgg tagttttttg
      301 ccgctttacc gcccaggcac cagaagccac gaccattggt gaaacgcttc atagtgagcg
      361 tgttatcccg gtgctttttg ccataccacg gggccagaga cagcagcgac ggaatatcgc
      421 ggatggtagg ctcgacgtgg gttttcataa agttctcggc atcaccatcc gtcggcaacc
      481 agataagggt gttacgctgc ttatgctcta tgaagtaagc ataaacaccc agcagcattt
      541 ttgaataacc aacacgggca gacttcacca cattcacctc gcggatgtag tcgctgccca
      601 tcgcattcat gatagcccgc tgaaagggta gtgtttccca gcgcccttcc tggtatgcgg
      661 attctttcgg gagatagtaa ctggcatccg cccattcaac ggcagtctgt ggctccggcc
      721 tgaacagggc tcgcagcccg gcgcgtacat cacgccgcag aatatcaatc tgactgttcg
      781 atatattcac tcagcaaccc cggtatcagt tcatccagcg cggctgcttt gttcatggct
      841 ttgataatat cccgtttcag gaaatcaaca tgtcggtttt ccagttccgg aaaacgccgc
      901 tgtactgaga gagggatccc gtcaagaata ctggcaattt cacctgcgat ccgtgacagc
      961 acgaaagtac agaatgcggt ttccaccact tctgcggagt ctctggcatt cttcagctcc
     1021 tgggcgtcag cctgcgcacg cgtaagtcga tggcgttcgt actcaatagt cccaggctgg
     1081 agatctgcct cgctggcagc cctgtaatcc tcaacctctt tacggagttt ttcattttcg
     1141 atatcagctt ccctctgcgc ataccactga attgccgtgg cggtatcaaa tacagattca
     1201 acgcccttac cacctccgga gacgcaaggg agcccctgag actgccagcg ttcaatcgtt
     1261 cgcggatcca ctttgaaaat ttcggcaagt ttctttttat taaccttcat gaaacagtct
     1321 cacaacaaat acagggtccg acatgaaagt gcccgaaaat gactttttca ggcgttttca
     1381 tgtcggacct ttacggattc gatattagaa aaaacaaata gttatgttcg agaagtaccg
     1441 acatgatttt ccccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt
     1501 aacggcccgg atcgccggaa aggacccgtg aaaatgataa tgattatcag ttgcaacaaa
     1561 gtccagtttc ttccaccatc gcaccggacc agcgaccatg aggggacaac gccgcgctcc
     1621 gttaacgcgg taaaccccgg tgtgtatcgt ttttgattat ccccgcacac tcgcgcagag
     1681 gagtctccct gtcgggctgc ggtctctgtt aatacgggaa tacggcgaca ataccgcgca
     1741 tggataataa ggtcgctcaa cacactggct gtaatgcagc cgataccata cggcatttag
     1801 cggcattcat cgtacactca acggttagct cttcattcgt ggcattcacc tgaaaggtcc
     1861 gggagtgtaa ttgcgtacat ttaccactga acgaaccttc aacaagaaca cgaccacgct
     1921 gcaaaatacg gaacggaatt gttccctgaa aaggctttac ggttaccagt aatttcttca
     1981 tgcattctcc ggataacaaa aatactagtt aatacactga gtacggatat attcctgaag
     2041 cattctcaat gcagcctggt cgctgatgat tccgtctctg ataccgagaa cgtttcgtcc
     2101 agcaaccgga gagagttcga cggcggcatc attgcccacg ccggaggtgc cggtggcttc
     2161 acgcacggta ccggagcagg tggcgttgat ccgcaggcgc ttacgaccag cggcaacatc
     2221 agcacgcaga gtttcatttt cagctctcgc atcggctaat tccctcgagt attttgcatc
     2281 gagcgcagca acatcgcgct ggcgcacctg catatcagta atggttgcgt ttgccagctc
     2341 cagctctctg gcttttttat cgcgctgcgc tttgtaggtg atggcgttat cgcggtaatg
     2401 attcagcccc agactaagcg caccacagac caccagcaga ataacggtaa acgcggaaag
     2461 cattcggttt atgctcaccc cagcagcccc gacgaagata acatcatcca gcccatggaa
     2521 agaaaaagag caaccagcat tagtgaaaat gaaatgccga cgattacaca gaggatcttc
     2581 gccagcatta tgagtttgtc tgacatgttt aatcctcttc acgatttcaa cgcaatgacc
     2641 agttttgcca gcccatacag catcggagac acagcaatac cgaccgccac ccacttaata
     2701 gcaaaagcct gtgctctgct gatgtcatca gttactggcg ctttcagttc aaggccgttt
     2761 ttcatagtca acctcaacag aattcgttta tacttttcca tgttctccct tgccttatcc
     2821 aaggtcagaa acacaaaacc ccgcttgctg ccaacaaacg tggtttttac ttttattcac
     2881 ttaggttttg tcagttcgca ggatttcgtg ttatccgtcc gtgtgagcaa acctcatttt
     2941 tcagcaaaat attcttctta tctgtcgatt ccccagcacg ccagcgcgct ctcctggtca
     3001 cgacgggata cctgaccgta acagttgttt gaacgaatac ggcagtctct gccaccgtcc
     3061 ttaatccacc agcgaatcgc ttcgcaggca ccttttcgat cacctgcatt aattcgtctg
     3121 taaaacgtcg acgggaaaca cttaccggga ccaatgttgt acggacagaa tgacgcgatc
     3181 cccgctttct ggggttcggt cagcggcact ttgatgtttt tctccaccca tgccagcgcc
     3241 ttatcacgct caatggcgtt aacccggtcg catttttcct tcgacaactt catgcccgga
     3301 acgacaggtt taccatccac caagatggca ccgcggcaga tggtccagat
//
LOCUS       DAZUGK010000310         3325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZUGK010000310 DAZUGK010000000
VERSION     DAZUGK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.312"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            515..740
                     /gene="ykgL"
                     /locus_tag="ACHY5W_004579"
                     /pseudo
     CDS             515..740
                     /gene="ykgL"
                     /locus_tag="ACHY5W_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(775..915)
                     /gene="ykgO"
                     /locus_tag="ACHY5W_004580"
     CDS             complement(775..915)
                     /gene="ykgO"
                     /locus_tag="ACHY5W_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFS4449319.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(915..1178)
                     /gene="ykgM"
                     /locus_tag="ACHY5W_004581"
     CDS             complement(915..1178)
                     /gene="ykgM"
                     /locus_tag="ACHY5W_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4449320.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1542..1643)
                     /gene="ykgR"
                     /locus_tag="ACHY5W_004582"
     CDS             complement(1542..1643)
                     /gene="ykgR"
                     /locus_tag="ACHY5W_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFS4449321.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2117..3259)
                     /locus_tag="ACHY5W_004583"
     CDS             complement(2117..3259)
                     /locus_tag="ACHY5W_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4449322.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQVFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          934 a          676 c          715 g         1000 t
ORIGIN      
        1 ttggcaatat aacgttcaat tcttacttgc attttatatt agccaggaac tttgccctga
       61 ggtgttagga atagtcttaa tgtgttgtgt aatggactag taaaaaaaca agtttaaatg
      121 ataagaaata ggatataaaa ctatcatgag tcataattaa agatgatttg ccaatcagta
      181 aggtgtttta atggattaac atgccgcaca aaatggttat gggtgttcat cccaataagt
      241 gatgaaaaat taaacaggtg taagtacgtc atctgtccgg taagccccct aagagccaaa
      301 ttaagtgaag ttaaatgaag actgccagtt gtcacaggaa cgatggcctc tttgccaggg
      361 cactgcattc agtcatctgt gcgcgttact ctaactggca ctcgaacgtc ggcgatgggg
      421 ttaccggtgg atcaggccgt tcctgtggcg taaacgtttt tatgtcagta ccaacagatt
      481 taccacattt atcatcttaa cagtttgagt gtaaatgcag gctgcgtcgc aaagggagga
      541 aactgagtgg cgtgttcagt ctaaaagagg gttgatgcca gcatacaggg cgaagctggt
      601 caacaagtca acattaatat aatggaatat tcggagagaa acgtcaggca gttgacctta
      661 aatgaacagg aagatacatc cccaggaaaa ttaatgctgg tgtaataaat acaccaacat
      721 taatccggtc tgattattaa tcagacaaat atcgttaatt cgtcgaattt taaatcaacg
      781 ttttttctta cgaccctgaa cggccttaaa acgtggatta gatttacaaa tcacatacag
      841 ccgtcctttt cgcttcacaa tctgacagtc tggatggcgt tcttttgcgg tacgcagaga
      901 gttaaggact ttcatcatgc cccctttttc gtgctaacaa aacgaccaaa acgttgggtg
      961 aaccgtgcaa catttccttc tgatgtcact gttctcagct tccctgtata gaacgggtgc
     1021 gatttcgaag agacatcaat tgttacgtac ggatacgtta cgccatccag ctcaatctca
     1081 cggtctgttt tgatagtcga gccgatttta aagtactcat caacactggt gtcgtggaac
     1141 accacagtac gatactcagg atggatattg ggcttcatca tttttacctg ttatgttata
     1201 acataaccat aaagatacat gctcatccgg atcatttcaa cacccaatcc ctgtgaccat
     1261 tagtggtatg tggaatttcc tcaacgggga agtctggcat tgttaacagc cattggcgac
     1321 gctcccataa acgcctggat gagtgattga tgatagtttc gctgagagta tggatttttc
     1381 gagaatggag gttatctcca ctttactggc cgatgattta tcgcagtatg attgattacc
     1441 tgacagcgtg tattcctctc ccacggatat aataattact tacaaaaagg gagaggatgc
     1501 atattttaaa tatcactgaa gtgaacagtt tattgccgtt attaatagaa atggagaaat
     1561 aaataggcgt attctacaat tgcgacaaaa acaacgatat taatcagttt atgactgatt
     1621 tgttgtactt tattctcttt cattggtact tcctcgcttt aaaaaagagc gcacttcgta
     1681 agtgccctta tataaataac gagtttggtc aaccaatttt ttgacatgta tcacaaattt
     1741 gaatagatgt attacatcgg ctatctttta ttgcaccaac gtcattgata tatgtcgcct
     1801 gaagtcagtt ccgggaatga gtctgatctc aagactggcc cagtcctggc gttgattggc
     1861 gctgaggagc atatcgcatc tcatcataat gtcgtatctc ctggggtgtt atacaagata
     1921 tcgttgttgg taacctgggc gaggaattga gctctattaa accgtcaact atgccggata
     1981 catactggat tacactgcag gcacgcctta tgagagaacg tgccgcagtg acgggttaat
     2041 tatctgaaag aatttgtgag gctgtatcgg ttactcattg atttgatagt tttactctcg
     2101 ggagagtaat agatatttaa tccattaacg gaaaccagcc agttcctttc gatgcctgaa
     2161 tttgatccca tagtttatcc ggaatggtta attcaggaac aagttgtgga ttaagggttg
     2221 tttgaatttc atggcctcta cctgattcag ctaaagtcac ccattctgga tttatcacca
     2281 gccctttacc gattgcaaca agtggcaggc cagtggaaat tgcttcttga gcctgagacg
     2341 gtgtgcgtat tttacctgca gcgattaccg gcactctgcc tgcaatatga tttaaaatca
     2401 attcaatagt tcgggggcca tttggcgact caacagggta gctatcgtta atactgacca
     2461 gcgaggtatg tatatacgaa ataccggatg aaatgagccg atcaaggagt ttataagtat
     2521 cctctatccg cagcccccct gttgcagatt cttcaggaga tattctgtaa ccgatggcaa
     2581 agggctttgt cgcatattca tagactacat tttttacttc ctgtaatacg gccagcggga
     2641 agcgcatacg tccttcgaga tctcctcccc atcggtcatt acgctggtta aatagagggg
     2701 agaagaaatt ctgtagaagg aatccgtgcg caccatgaag ttcaatgcca tcaaaacctg
     2761 ctttaatcgc cctttttgtg acatctccga atgctctaat ggtttcctga atttcgtttt
     2821 cagtcatttc cctgctttgt accactctct tcataaagtc accagatttt acgcttgatg
     2881 cgctggcact aataacatcg ttatttggta caagctccgg gatggcttta ttaccggcgt
     2941 ggaaaacctg caaaatagcc ggagcacccc cacttttggc tgccgctgct aacttttcaa
     3001 ggctgttaat gaaacggtcg tcatacgcgg caaattcatg agtaaaccca atgccgcttg
     3061 gcgtgacata ggtacatcct gttattacta aaccaacatt ttgtgagcga cgtttataaa
     3121 attcgagttc ttgctcagag atggttccat caggattcgc tgaccaggtc gtcataggag
     3181 ccataacgat acgattacgt agctttattt tttctgtcag cataaaagga gaaaacaatg
     3241 atgggtgttt atttgtcata acaatacctc aaatgtttat ctgattttga tgcgtatgtt
     3301 ttaactggtt agttgttgcc agctt
//
LOCUS       DAZUGK010000311         3322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZUGK010000311 DAZUGK010000000
VERSION     DAZUGK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.313"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1299)
                     /gene="ureC"
                     /locus_tag="ACHY5W_004584"
     CDS             complement(<1..1299)
                     /gene="ureC"
                     /locus_tag="ACHY5W_004584"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFS4449323.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNGSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLV"
     gene            complement(1292..1612)
                     /locus_tag="ACHY5W_004585"
     CDS             complement(1292..1612)
                     /locus_tag="ACHY5W_004585"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFS4449324.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(1621..1923)
                     /locus_tag="ACHY5W_004586"
     CDS             complement(1621..1923)
                     /locus_tag="ACHY5W_004586"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFS4449325.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(1933..2713)
                     /locus_tag="ACHY5W_004587"
                     /pseudo
     CDS             complement(1933..2713)
                     /locus_tag="ACHY5W_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
     gene            3094..>3322
                     /locus_tag="ACHY5W_004588"
     CDS             3094..>3322
                     /locus_tag="ACHY5W_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449326.1"
                     /translation="MTEHRSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGP"
BASE COUNT          758 a          846 c          888 g          830 t
ORIGIN      
        1 caccagatcc gccagttttc ccacttcaat cgagccgact tcatgagcaa taccgtgggt
       61 taatgccgga ttaatggtgt atttagcgat atagcgcttc acgcggacgt tgtcgttatc
      121 accactttct tccggtaacg ggccgcgctg aactttcatc cggtgtgcca cctgccaggt
      181 tcgtaacact acttctccga cgcgtcccat ggcctgcgaa tcggacgagg tgagggagaa
      241 cgcgccaagg tcgtgcagga cgtcttccgc ggcaatggtt tcctggcgaa tgcgcgattc
      301 ggcaaaggct acgtcctcgg cgatatccgg gtcaagatga tggcaaacca tcagcatgtc
      361 cagatgctca tcaatagtgt tgacggtata gggtagcgtc ggattggttg aggaaggcag
      421 aatattgggg tgcgcgcagg cggtgataat atccggagca tgaccaccac ctgcaccttc
      481 tgtatggaag gtatggatag tgcgcccgcc gatggcagtc agagtatcct caacgaatcc
      541 tgattcgtta agcgtatcac tgtgtagcgc aacctgaacg tccatttcgt cggctacagt
      601 cagtgcgcag ttgattaccg caggtgtagc tccccagtct tcgtgaattt tgaggccgat
      661 aaccccggcc gcgacctgct cacgaagcgc atccggattg gagccattgc ctttacccag
      721 caacccgata ttgaccggca ggctgtctgc agcctgcagc atttgataaa tgtaccatgg
      781 tcctggggta caggttgtgg cgttagaacc cgctgtcggg ccagtaccgc caccgatcat
      841 ggtggtaatg ccggatgtca gcgcttcttc agcctgctgt gggcagatcc agtgaatatg
      901 cgtatcgacg ccacctgcgg taacgatcct gccttctgcg gcaataattt ccgtggatac
      961 gccgattggg atagtgacgt tgggttgtat atcaggatta ccggctttgc cgatagcaaa
     1021 tatccttcca tctttgacgc cgatatcggc tttaacgatc ccccagtaat cgatgatcag
     1081 ggcattggtc agcaccagat cagcgcagcc ggcggagagc atttgcccct gtcccatacc
     1141 gtcgcggatt actttaccgc cgccgaattt gacctcttcg ccgtaggtag ttaaatcatc
     1201 ttcgacctcg atccacagct cagtgtctgc cagacgaatt ttatcaccgg tggtagggcc
     1261 gaacatgtca gcataggcct ggcgtgaaat attactcatc atttgcctcc agctcaccca
     1321 ttatatctcc gcggaaaccg tgcacgatac gggcaccgct cagcgccacc agttcaacct
     1381 cacgcttttg gcctggttcg aagcgcactg cagttccggc ggcgatattg agacgatagc
     1441 ctcttgcctt ctggcgatcg aacttcagtg ccgggttaac ctccgcgaag tgataatggg
     1501 aaccgacctg aattggtcgg tctccgtgat tttcaacgat aatactgcag gtagcccggc
     1561 cgatgttaag ctctatatag ccaggtttaa ccttatattc tccagggatc atctgttacc
     1621 tcagattatc ggattatgga cggtaaccag cttggagccg tccggaaagg tggcttcgac
     1681 ctggatatcg gggatcattt ctggtatgcc ttccatgacc tgctcgcgac tcaggacatg
     1741 ccgtccttct tccatcagcg cagcgacgct tttgccgtcg cgagcgccct ccattataaa
     1801 agcgctaatc agggccacgg attcgggata gttaagtttc aggccgcggg ccagacgacg
     1861 ctctgccagc agtgcagcgg taaacagtaa tagtttgtct ttttctcttg gagtcagttc
     1921 catagcgttc tcttaagtca gccagatccg gggaagtacc ggtgatttgt cagtcagttg
     1981 cggccgcata aattgccaga tatcacgcat tacctgctag caacgcagat tatcatcact
     2041 taaaaagcga actgtcagta gaccatcggt gagcgtggct ccggcgtagt tttccagtgg
     2101 tgtgagtttt tcgcgtatgt cttccagttg cgtttcattc cccgggtaga acagcatcgt
     2161 tcccacccat ggtttccggg caacgcaggt aagttctcca ttggccacgt gcagacgctc
     2221 aatcaacagc ggagaaccgt cgacccatat ctccagacgg ttagcgagtg ttccgtggct
     2281 gaaggtttct ccgatgaccg gacgtccaag gcacagaaga tcccaggcca gcagtgtact
     2341 ggaggacgac agatgaaaca cggtatgcaa cgtggcgtga gcgccgggaa aaaaaaatcg
     2401 aatcctgtgg caaccattcc agcgttgcgt tattgcacag cgtcaggttt tgctgaaggt
     2461 gcgcctgcgc gccgctgctg cggtaaaatt tactggcacc aggcatggtg atcagcgtat
     2521 ggcaatcaga gtcaattatt gcgttgatga tgagtttgtc accgccgacg ataccaccgg
     2581 gtggatgaag cagacagagg tggcaggttt tttcttcggg ataaaaagga cgctgaacag
     2641 tgagcggacc gacatgatga cgggtggtga gcgtggtttt cccgtgcaga cgctgaaaac
     2701 ggagatccag catggcctgc cagccttttt tattttgtgg taacgcggta ctgagcaatc
     2761 ttacgcccct gataaataaa gataaaacga cagtaaagag ccggatttcc ttgttatata
     2821 aaagatgatg cctttccagt gagttttcgt ctgaataaaa cgaatgtttg tttccgtcat
     2881 gaagcgttcc ggctcccggg gctatacatc gccagaaaat caatgcatat tgtcgggatt
     2941 atccagagaa tcaggcagaa agatccgaca atgggctgtg attatgccat ctggctcaga
     3001 atgatatcaa ggtgttatgg ggataacttg ctgatacaat agcagtatga cagattcatt
     3061 aatgcggaaa ctgctcactg aaacagagtg ttcatgactg agcaccggtc gggtcatgca
     3121 gatgataatt tccccttttc ttggggaagc gacagtattt tcatgcatgc ctgtcttcag
     3181 tcatacgggg agagcatctg tgatacagtc catacatttg ttgcggacgt cgctcctgag
     3241 tcactgactg gtctgcaaac agtttatctg tacacggcag ggcttctgtg tgatatcgtt
     3301 tttcatatcg agggggggcc tc
//
LOCUS       DAZUGK010000312         3321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZUGK010000312 DAZUGK010000000
VERSION     DAZUGK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.314"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1222)
                     /locus_tag="ACHY5W_004589"
     CDS             complement(<1..1222)
                     /locus_tag="ACHY5W_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4449327.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGVAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLVATQERLNRNIAARS
                     AAQNALNSTTAVGSR"
     gene            complement(1270..1479)
                     /locus_tag="ACHY5W_004590"
     CDS             complement(1270..1479)
                     /locus_tag="ACHY5W_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFS4449328.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGVNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(1575..1949)
                     /locus_tag="ACHY5W_004591"
     CDS             complement(1575..1949)
                     /locus_tag="ACHY5W_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS4449329.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     REVLDGGDAGDETLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALDLIADAESARKK"
     gene            complement(1955..2671)
                     /locus_tag="ACHY5W_004592"
     CDS             complement(1955..2671)
                     /locus_tag="ACHY5W_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS4449330.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFMKNPEKTLTVN
                     TGALLTMSVSVNGGTPPYKYAWKKDGQPVEGQTTDTFSKANAQSGDKGAYTCMVMDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(2730..3074)
                     /locus_tag="ACHY5W_004593"
     CDS             complement(2730..3074)
                     /locus_tag="ACHY5W_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFS4449331.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQSYIPENR
                     CYRATLEFQVTV"
     gene            complement(3071..>3321)
                     /locus_tag="ACHY5W_004594"
     CDS             complement(3071..>3321)
                     /locus_tag="ACHY5W_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4449332.1"
                     /translation="SRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGT
                     VNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          714 a         1093 c          804 g          710 t
ORIGIN      
        1 gacgtgagcc caccgccgtt gtactgttca gcgcattctg ggcggcgctt ctggcagcaa
       61 tatttctgtt caggcgttcc tgtgtggcca ccagacgggc ctctgcagca atctgcatct
      121 ccgtcccgcg ggctgccgcc acggcctgct gagcacggta cacggctgcc cttgcccgcg
      181 ccgtggcaat ctgcgttccc ctgaactgtg cttccgccag tgcaacttca ttacgtgcag
      241 ccgtcacaag tcctgccgtg gcagacatcg ctccggaggc catattgcca aagtaccggg
      301 caaccccgac ggcaaccagc gcccccgcgg ctgttgccac attatcaata ttaccggcaa
      361 caccgttcag cacgccggag agcgttttcg tcgctccgct ggcctcattc gcaccgccca
      421 cccaggccat aaaggcgttt tccacctttg tgatcccgtc agagaccgtt tctggcatgg
      481 cggcatattc atcacgcaat gtccccagct ggctgattaa cgcgggcacg actttatccg
      541 ccgtcagttg accatcgtcc gccatcgcct tcagatcttt acgggccacg cccatgcctg
      601 cagccagtgc acgaatgatc cggtcaccac tttcattgac cgaattaaat tcctcaccgc
      661 gcaacacacc ctgtgccagc gcctggctga actgggtgat caccgaaccc gcctcagccg
      721 tactggcacc ggagattttc agccccgtgg aaatggcctc cgtcaccttc agcacatcat
      781 cagcactgta accatattca cgcatcgagg cagccgaacg ggcaaacagg gccgcattat
      841 ccgaaaaagc ggtacctgtc cgctgactga tatccatcag cactttctgt gatgacgaaa
      901 attcatcgga tgactgtgat gcctgtttca gacgggcatt tacggaactc cactcatccg
      961 ccagtgaaat caggtgtccg gaggcaaagg caccggcaaa tgcgccagcc actccgacag
     1021 cagaaccgcg aatttccgtc aactggctgt tcagttctgc cagggcgcgt cgctgctccc
     1081 gggctgccgc agcggcctga cgcccgccat tttgcagggt ccggtaatat tcactgccca
     1141 tgcgggaagc acgctggatc tccgactgga atgactgtga atttgccgaa attttgataa
     1201 tcagttcacg taacgtcgcc attaaccttt ctccgggcgc aaaaaaaccg cctcagcgat
     1261 tctcatcatt tatgactgtg ctgcaaggct cagcgcgtct tccagcgccg caaacggatc
     1321 cacctccggc ttgtcctcat cctctcccca gcagagcatg gcgtccttca gtgcaacatt
     1381 caccccctgc gccccgaaaa ccgctttcac gatctgcgca ttacggatat ccccgcgctc
     1441 atcacccagc ggggatatcc tgtcgaactc catccacatc atcgcctcgc tcgcactcag
     1501 gctatgccgc agttcggata aggtgcgccc cagacggagc gcaagtcgca tcagaaagcg
     1561 aatttccggg cgggctactt ttttctggcc gactctgcat cagcgatcag gtccagtgcc
     1621 tgacgcagca agcgggcatg taccggacca tagacggcca gcacctgctc acggtcgtcc
     1681 ggagtgaaca cccgctgcag gtccgtatca cacagaacat cgcagaacag cgtcacatcc
     1741 gcttccagat tacggcgggt tttcgccacc accgacaggg tctcgtcacc cgcatcaccg
     1801 ccatccagca cttcccgcca cagataccag gcctctgccg aaggctcccg cagcaccacg
     1861 ctgacatttc cccattccgg caccttcacc gttttatgac ggaaccccga cagcctggcc
     1921 agtgccagtg ttttcagatc ttttgccata agccttatcc gcccgcacca ttaaccgtta
     1981 ccgtacacgc gtcagaggta atgctctgcg gctgttctgc agaatccatt accatgcagg
     2041 tataagcccc cttatcacct gactgcgcat tggctttact gaaagtgtca gtagtctgtc
     2101 cctcgaccgg ctgaccatcc ttcttccagg cgtatttata aggcggcgtt cccccgttga
     2161 cactgactga cattgtcagc agcgcacctg tattcacggt aagtgtcttc tccggatttt
     2221 tcataaacgc cagcggtacc acataagaaa caggcttacc tttcagtcgc agtgagaacg
     2281 ttgcagccac cacgccgttg gtaccggatg accaggtgtg ctgacgcact tccgccagga
     2341 acttaaagcc cttaccggac ggaaactgca ccttaaacgc atacaccgtg tcattatcat
     2401 aggcatcacg cagggcattc tgagcctgat taagataaaa gttgcccgac atggaaatct
     2461 cggacgatgc gcccagaccg ttgatattct cctgctctgt ggagcagagc gtggtcacat
     2521 caatatcctg tttctgcccg gcggtgaact ggacctcctt gatggtgcag tccagacgca
     2581 gatattccgc cttctccatg gtttcagcag tcgccggggc agatgaaatc atcacctgcg
     2641 tcagctgtga acgttcatac aaagcagaca ttctgcctcc tgataataaa aaacccgcac
     2701 gcggcgggtt atgggttctg ttgaaaaaat tacaccgtga cctgaaattc cagggttgca
     2761 cggtaacagc ggttttccgg aatatagctc tgcatttcac tgacggatcc cggggccagc
     2821 agcattatgg cttcacgggc gtcctgacgt atctgacgcg cctgcgtcac agtcccggca
     2881 taaacgtcta tctgcaccga cactgaggac tccgcctgcc cacccatcac gtccgccgac
     2941 accgatgaaa tcaggctgaa aaccacccac ggaagcgcca ccgacggcct gccatccagc
     3001 agggggacca catacgggta cacctgcccg ccggcaagat gtgccagatg aggatacaaa
     3061 tccgcctccg tcatcgtctc agtacctcat caatggccct gttcatccga gcaatcgcca
     3121 cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg cacaaacggg tgcggtggca
     3181 tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg ttatccgcct
     3241 tcatggtgtt atcgctgtta ccggtgtccg gattaacacc ccggatatgc acaccggatt
     3301 ccatcccgcc atcgcgggag c
//
LOCUS       DAZUGK010000313         3242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZUGK010000313 DAZUGK010000000
VERSION     DAZUGK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.315"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            453..962
                     /locus_tag="ACHY5W_004595"
     CDS             453..962
                     /locus_tag="ACHY5W_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4449333.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            934..2862
                     /locus_tag="ACHY5W_004596"
     CDS             934..2862
                     /locus_tag="ACHY5W_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4449334.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWIQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYC
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2846..3052
                     /locus_tag="ACHY5W_004597"
     CDS             2846..3052
                     /locus_tag="ACHY5W_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFS4449335.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            3049..>3242
                     /locus_tag="ACHY5W_004598"
     CDS             3049..>3242
                     /locus_tag="ACHY5W_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449336.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNG"
BASE COUNT          790 a          735 c          935 g          782 t
ORIGIN      
        1 aaatcggatg ttgcgggttg ttgttctgcg ggttctgttc tttgatgaca taatgttgcc
       61 ccgtattcag tgttgctgat ttgtattatc tgaagttgct tttacgctaa tttgatgcag
      121 atcaattaat acgatacctg cgtaataatt gattatttct cgtggtttga tggcgtacac
      181 acatgtcgtg ataaacctca tgtagatgat aattattatc attttgcggg tcctttccgg
      241 cgatccgaca ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc
      301 gggatccatg tccggtttct cttcaagtta actatatgaa aaatataaaa acaggtcttc
      361 tgtgaaccgg acatgaacaa aaaacagaca tgtaaaccgg acatgaccgg ttttgttgtg
      421 attgtgaggt gagagttttt gcgaggtgag gagtggctac gcagactgaa gttgccaggc
      481 atttaagtct gaccgatcgc cagcttcgca gattgcagaa attgccgggt gccccgatat
      541 cgaataagcg agggcaactg gatctggatg cctggcgcga tttttacata tcgtatctga
      601 ggagaagtaa aaacgatgtg cctgatggcg atagcgaaga cgactatgag gagaaattgc
      661 ttattgccag atgggaactg acagcagaac aggctgttac acagcagtta aaaaatgagg
      721 tgtcaaaagg aaaacttatt gacaccgggt tctgtatttt tgccctcagt aagctggcaa
      781 tggcgttatc cagtacgctt gattccatcc ctttatccat gcagcgacag tttcctgatt
      841 taacaccgcg ccatcttgac catctgaaaa cccttattgc taagggggca aatcagtgtg
      901 cgcgggcagg ggataaatta ccggatttac tcgatgaata tatcagagca acaactgaat
      961 aatatgatgg ctgccgtttc ggttgcgctg cagcctctgg tcagggttgt accaatgacg
     1021 gcagttgaat gggctgatca aaattattat ctgcctaaag aatcctcata cggtgagggc
     1081 gaatggaaaa cgctgccatt ccagatcgcc attatgaact gtatgggtaa cgaccaggtt
     1141 cgcacggtta acctgattaa atctgcccgt gttggctata caaagatgtt gctgggggtg
     1201 gtcgggtatt ttattgagca taaatcccga aacagtctgc tttttcagcc cacggattct
     1261 gccgctgaag attttatgaa gtctcacgtg gaggcgacga ttcggaacgt gccatgcctg
     1321 aaagaccttt ccccatggct gggtcgtaaa catcgtgaca atactctcac gctgaaacgc
     1381 ttttcatcgg gcgtcggttt ctggtgcctg ggcggtgcag ccgccaaaaa ctaccgtgaa
     1441 aaatccgtgg acgtggtctg ctatgacgag ctttcctcgt tcgagccgga tgtcgaaaaa
     1501 gagggctcgc caaccctgct gggggataag cgtattgagg gctctgtatg gccaaaatcc
     1561 attcgcggct cgacgcctaa aatcaaaggt acctgccaga tcgaaaaagc ggccaacgag
     1621 tcggcgcatt tcatgcgttt ctatgtgccc tgcccgcact gtggggaggc gcagtatctg
     1681 aaatttggcg atgaatccac gccttttggc cttaaatggg agaaggacag ccccgaaagc
     1741 gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgagct tgaccagagt
     1801 aacgggcgct ggatctgtga aaacacgggt atgtggaccc gtgacggcct gatgtttttc
     1861 agcgcccggg gggatgaaat tccgccgccg cgctccatca cgttccatat ctggacggcg
     1921 tacagtccgt tcaccacctg gatacagata gtctatgact ggctggatgc actgaaagat
     1981 cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg agacctggga agaggccgtg
     2041 ggcgaaaaac tcgatcacca ggtactgatg gataaggtgg tgcgttacac ggcggcggtg
     2101 cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg ttttgagatg
     2161 tatgtctggg gatgggcacc gggagaggaa gcctttctgg tggataaaat catcattatg
     2221 ggccgtcccg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa caaaaaatac
     2281 tgccatgcag acggaaccga aatgaccatt tcccgtgtct gctgggacac cggggggatc
     2341 gatggtgaaa ttgtctatca gaggtcaaaa aaacacggtg ttttccgggt gctgccggta
     2401 aaaggcgcat ctgtctatgg caagccggtg atcaccatgc cgaaaacccg caatcagcgg
     2461 ggcgtgtatc tgtgtgaagt ggggacggac accgcaaaag aaattctcta tgcccgtatg
     2521 aaagccgatc ccactcctgc ggatgaagcc acgtcgtatg ccatccgttt tcctgatgat
     2581 ccggagattt tttcgcagac agaggcgcag caactggtcg cggaagagct tgtggagaag
     2641 tgggaaaaag gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa cgaagcgctg
     2701 gactgcctgg tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg gcagcttgat
     2761 ctggctgtac tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct gaaagaactg
     2821 gcagcgaagc tgtccggagg agtgaatggt tacagtcgct gaactacagg cgctacgtca
     2881 ggcgcgcctt gatttattaa ccggtaaacg ggtggtgtct gtccagaaag atggtcgcag
     2941 aattgaatat acggcggctt ctctggatga gcttaaccgg gcgatcaatg atgcggagtc
     3001 ggtactgggg acaacccgac gtcgccgtcg tccgctggga gtgaggttat gaaacgaacg
     3061 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacgggggc
     3121 ggcgcaggat ttggcgggca aatggcggag tggttgccac cggcgcagag tgccgatgca
     3181 gccctgctgc ctgcgttgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     3241 gg
//
LOCUS       DAZUGK010000314         3239 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZUGK010000314 DAZUGK010000000
VERSION     DAZUGK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.316"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(124..3012)
                     /locus_tag="ACHY5W_004599"
     CDS             complement(124..3012)
                     /locus_tag="ACHY5W_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4449337.1"
                     /translation="MHSWKKKLVVSQLALACTLAIASQANATTYDTFGYHDDAATVFG
                     WDDTTDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLNIT
                     NSNVHGMVTSYQVGYGDYIWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFTDT
                     YLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQNDLGNATYNT
                     EGHQWDNNIVVNDSTVTSGSLSEDEQSTSDRGHFGNSVEPSDYGNGASAADDVALAFI
                     DDVTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSAESSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWTSAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSVDNVVAATGNYKVRIDNSTGAGAIADYKGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT          868 a          732 c          765 g          874 t
ORIGIN      
        1 accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg cagtaatgcc
       61 cgtcaaatcc ttgacgggca ttatttagat taaattacca gtatttcttc ggagtgaaga
      121 atattaccag gtatatttaa cacccacgtt cgcggaccag tcttgatcta cgtcaccacc
      181 accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga actgagtacc
      241 cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac cgttcacatc
      301 gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag tcagagcctg
      361 atcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac gcatgctgtc
      421 gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac cagactggaa
      481 cagaccagaa atgctgccgt aaggagtcac gtaaccagca tcacccagtt tgaagtcgta
      541 accggctttc aaaccgaagc cccaagcgtc ggagttggtg ctaccgtcaa cgtaagtacc
      601 gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc taccatcaac
      661 aaagacgttg ttcgcaaagt gagcagaaga gtagatgtag gcagtctggc tgtcttgatc
      721 cacctgacca gaacggtcat tcatgtcacc tttagcgaag cctgcagccg caccgacgat
      781 ccacttagcg ttgttaccgt caattttggt atcaacaccg accatgatgc cgttaacatc
      841 ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta ccaccgaagt agcttaccca
      901 tgcgccgccg ttatcagcca ggccatgacg agagttggtc aaacgagtac caacggtgtc
      961 ttgttccagg ttccagatat tggtgttcgc agacgggatg ctcagcgcca tgttagcgta
     1021 gtcggtcagc tccatctgtt gcagaacaac ggtgttaccg cgctgttcag cctgataggt
     1081 gtatgcaccc aggtcagctt tgttggctgc agagaaggtc gcgttggttt tggtgtcatc
     1141 gatatagatg agctgtttgc ctttgtaatc agcaatcgcg ccagcaccag ttgagttgtc
     1201 gatacgaact ttgtagttac cggttgcagc tactacgttg tcaacagagc tgttgtcaac
     1261 ttcgtcgccg ttgttaatat cgttgttgcc attaacagtc aggtgaccat cagagttcat
     1321 cgcaataaca ccgtagccgt agttagcttt gctggtgtcg ttggtacgat cgttgacgag
     1381 gtctgcattc aggatgtagt cattgctatg aatgtcgaat acacctgcgt aaacgttgcc
     1441 gttgttatct ttaacgttgc cgctggtcag ttccagagta tcagttgcaa atacgctgga
     1501 gatgtcattc atattggtgt ttacatccag cacaccaccg ttagtaacgg taatagtgtt
     1561 ggcgtacagc ggtgctgatg tccaagctga agcagtatct tcaccgaagt taacggttga
     1621 gaaggtatca atggtcaggt gatcggtgtt aacttcaccg ttgacttcca ttgaagaacc
     1681 accgttcagg gtgatggtgt cagaagtcag ggaagactct gcactcacgt taacctgaga
     1741 accgctgttg atagccaggg tatcgatcag ggaagttttg gtggtttccc aatcagaacc
     1801 gttgttcagg acaacatcga atacgccgct ctggaatgca ttagcgttat caacacgatt
     1861 ccagccatct tcagagtaag aagcatacgg gttcaggctg tttggagcaa cgtcgtagaa
     1921 ggcttcgtca gcaacaacaa cgtcataatc aatattcgca gaaccaaccc atttggtgtt
     1981 gttcagagtc aggttcaggt gatcgacgtt ctgatcgtca gtagcccaac cgccatgagt
     2041 gtaagcagta gtggagttgt caatcaggtg acccgtcgcg tcaaagttag cgttccaggt
     2101 gctgtcaaat acaacgtcgc ccagtaactg agagttgttg aacgtaacgt tgttgaccat
     2161 acgataatca gaagttacat catcaatgaa ggctaacgca acatcatcag cagcagatgc
     2221 accattgccg tagtcacttg gttcaacaga attaccgaaa tgaccgcgat ctgatgttga
     2281 ttgctcatct tctgacagag aaccagaagt tacagtagag tcattaacaa cgatgttgtt
     2341 atcccactga tggccttcgg tattgtaagt cgcattaccc aggtcgtttt gaaccagagt
     2401 aatacctgca acatgggagt tgttgctgat gttgatgtta ctttcaacat ccagagtcac
     2461 agcggtacca agaccagcag ttaccaaacg atcataatct gtcttagagg ttttaccgtc
     2521 tgcatccaga taagtatcgg tgaagtagaa ggcgtcaaaa tcatcgtcaa tagtagaatt
     2581 agcgatgttc agcgtgaaga tgtcgccatc aacccagtcg tggccggtat agtcgctacc
     2641 gttggtccag atatagtcac cgtaaccaac ctgatatgag gtgaccatgc catgaacatt
     2701 tgaattggta atgttcaggg tattgccagt atctgtaccg taatcatggt tcagataata
     2761 ggtagaaatt acaccgttta cagtgtcgcc gctgaaagtg gtatcaaagt aaccatcagc
     2821 tgcgtggtca tatacataac caccataagt cagataatcg tgatcggttg tatcatccca
     2881 accaaaaaca gtggctgcgt cgtcatgata tccgaaggta tcataagtag tagcatttgc
     2941 ctgagaggcg attgccaaag tgcaagccaa tgctaattgt gatactacaa gtttcttttt
     3001 ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat caattatcaa
     3061 tgcatagaaa acatcttatg tttcgcgttt acgattatgc ggcgttatag aggaatggtt
     3121 caattattct ttcccctaag tccgattgtg gacatatata aaaacatcta tacacacaaa
     3181 gaataggctg acaaaaacac tgtatttcaa cacgttaatg aaatcattaa ttttgaacc
//
LOCUS       DAZUGK010000315         3176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZUGK010000315 DAZUGK010000000
VERSION     DAZUGK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.317"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..603)
                     /gene="mobB"
                     /locus_tag="ACHY5W_004600"
     CDS             complement(76..603)
                     /gene="mobB"
                     /locus_tag="ACHY5W_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFS4449338.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(585..1169)
                     /gene="mobA"
                     /locus_tag="ACHY5W_004601"
     CDS             complement(585..1169)
                     /gene="mobA"
                     /locus_tag="ACHY5W_004601"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFS4449339.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1239..1508
                     /locus_tag="ACHY5W_004602"
     CDS             1239..1508
                     /locus_tag="ACHY5W_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFS4449340.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1585..2571
                     /gene="srkA"
                     /locus_tag="ACHY5W_004603"
     CDS             1585..2571
                     /gene="srkA"
                     /locus_tag="ACHY5W_004603"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFS4449341.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2588..>3176
                     /gene="dsbA"
                     /locus_tag="ACHY5W_004604"
     CDS             2588..>3176
                     /gene="dsbA"
                     /locus_tag="ACHY5W_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFS4449342.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQ"
BASE COUNT          795 a          774 c          781 g          826 t
ORIGIN      
        1 acggatggcc tttttgcgtt ggtgcaaaaa aatgccggat gcgacgctgg cgcgtctgct
       61 ccagcctact cttgcttatc cgtcctgatt ttgcatccac tccaccacaa aatcagccag
      121 cccctcaaca tcattaatat ccagtaacgc gacatcaaga ttaagcggca catcactggc
      181 tacagcaata acatgcctgt ctatcactaa ttcttcaggt cgatgtccgg ctccatcgcg
      241 aaacagcaca atctttgcga tctcttcatg cttaaaccct tcgaccagaa tcaaatccag
      301 ctttgaggta tccattcgac ttgcgagaaa ctgtagatcc agctcttctt cgtctggtgt
      361 ttccgtcatc aaggcccatc gttgctggct ggcaacgatg gtttgtgccg cgccagcctt
      421 gcgcagctca tagctatctt tgcctggctt atcaacatcc atatcatggt gcgtatgctt
      481 aatcagccct ggacggatcc ctctggcgca taatgccggg atcagttttt tcaaaagcgt
      541 agtttttccg gtaccactcc acgcggcaaa ggcgagtaac ggtatcatcg tttttcctgc
      601 catcgggcaa gctcctctgg cgtattcacg ttaacaaatg catctttatg atcgctgaaa
      661 tcaaccgcat gaccgccagc cagacgcata aatgccatta cccggcgttc tcctgcttgc
      721 agatattcta gtaataaagg ctcaatagcg cggtttacca gagcaatagt cgggtgatcg
      781 cgttcaccgt catggaccca cacgacaggc gcatctttgc gctgatgatt aagccgggcg
      841 gctaaatcat ggggaatgta aggcgtatcg cacggacaaa acaaaaacca ctcaccagct
      901 tcctgctgca ttactgaaag catccctgcc agagggcctg ggtaatccgc cagtgaatct
      961 tcaatcactt tcagaccgcc tgcctgatag atttcctgat gacgattagc attaaccacg
     1021 acgtgagaga gctgcgtcat aagcgcgtca gcgacatgtt gccacagcgg tttgccattt
     1081 aagtccagca atcctttatc tacgccgccc attcgtctgg ctttaccgcc tgccagcaca
     1141 acgcctgtta tcgtcgtcat cagattcacc gatatcgcct cttttattgt gggattgacc
     1201 ctgctaacgt gtctgtctca agagtaagga gcattactat gaaatgtaaa cgtctgaatg
     1261 aagttattga actcctccag ccagcctggc aaaaagagcc agaccttaac ctgttgcaat
     1321 ttttgcagaa actggcgaaa gagtcaggtt ttgacggcga actggcggat ttgacggatg
     1381 acattctgat ctatcacctg aaaatgcgtg attccgcgaa agatgcggtg atcccgggtt
     1441 tgcagaaaga ttatgaagaa gacttcaaaa cggcgctgtt acgcgctcgc ggcgtaatta
     1501 aagagtaaaa gcttgtaagc ggcgccacca aaatcatcgt gaaatgatat ccttcatcat
     1561 tcgtaatgtt ttccggatga tgggatgaac aacagcgctt ttactttcca gacactacac
     1621 ccggatacca tcatggacgc tctgtttgag caagggatcc gggtggattc cggtcttacc
     1681 ccgcttaaca gctatgaaaa ccgtgtctat caatttcagg acgaagatcg tcgacgtttt
     1741 gtcgtcaaat tttatcgccc tgaacgttgg acagccgatc aaatcctcga agaacatcaa
     1801 tttgcgttgc agctggtaaa tgatgaagtt ccggttgcag cacctgtggc ttttaacggt
     1861 cagactttat tgaatcatca gggattttat ttcgctgttt ttccaagcgt cggtggtcgc
     1921 cagttcgaag ctgataatat cgatcagatg gaagcggttg ggcgttattt agggcgtatg
     1981 caccagacgg ggcgcaaaca gctttttatc catcgcccga ccatcggtct gaatgaatat
     2041 ctcattgagc cacgcaagct gtttgaggac gctacactga taccttccgg gttgaaagcg
     2101 gcattcctga aagcgacaga tgagctgatc gccgccgtta cagcacactg gcgggaagac
     2161 ttcaccgttc tgcggctaca tggagactgc cacgccggga atattctctg gcgcgatggt
     2221 ccaatgtttg ttgatctgga tgatgcacgt aatggtccag ctattcagga tttgtggatg
     2281 ttgctcaatg gcgataaagc cgagcagcgg atgcaactgg aaactattat tgaagcttat
     2341 gaagaattta gcgagttcga caccgctgaa atcggactga ttgaaccttt acgcgccatg
     2401 cgtttggttt attatcttgc ctggctaatg cggcgttggg ctgatcccgc gttcccgaaa
     2461 aatttcccgt ggttaaccgg ggaagattac tggctacgac agacggcgac ttttatagaa
     2521 caggcaaaag ttctacaaga accccctttg caattaacac ctatgtatta atcggagaga
     2581 gtagatcatg aaaaagattt ggctggcgct ggctggttta gttttagcgt ttagcgcatc
     2641 ggcggcgcag tatgaagatg gtaaacagta cactaccctg gaaaaaccgg tagctggcgc
     2701 gccgcaagtg ctggagtttt tctctttctt ctgcccgcac tgctatcagt ttgaagaagt
     2761 tctgcatatt tctgataacg tgaagaaaaa actgccggaa ggcgtgaaga tgactaaata
     2821 ccacgtcaac ttcatgggtg gtgacctggg caaagatctg actcaggcat gggctgtggc
     2881 gatggcgctg ggcgtggaag acaaagtgac tgttccgctg tttgaaggcg tacagaaaac
     2941 ccagaccatt cgttctgctt ctgatatccg cgatgtattt atcaacgcag gtattaaagg
     3001 tgaagagtac gacgcggcgt ggaacagctt cgtggtgaaa tctctggtcg ctcagcagga
     3061 aaaagctgca gctgacgtgc aattgcgtgg tgttccggcg atgtttgtta acggtaaata
     3121 tcagctgaat ccgcagggta tggataccag caatatggat gtttttgttc agcagt
//
LOCUS       DAZUGK010000316         3113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZUGK010000316 DAZUGK010000000
VERSION     DAZUGK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.318"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            254..1486
                     /locus_tag="ACHY5W_004605"
     CDS             254..1486
                     /locus_tag="ACHY5W_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS4449343.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWYRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1471..2109
                     /locus_tag="ACHY5W_004606"
     CDS             1471..2109
                     /locus_tag="ACHY5W_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFS4449344.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     SHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2188..2457
                     /locus_tag="ACHY5W_004607"
     CDS             2188..2457
                     /locus_tag="ACHY5W_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4449345.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2478..>3113
                     /locus_tag="ACHY5W_004608"
     CDS             2478..>3113
                     /locus_tag="ACHY5W_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449346.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTS"
BASE COUNT          823 a          645 c          848 g          797 t
ORIGIN      
        1 atatttgaat tgttcaccat ctgcatcata ttttcttatt tacaagttga aaggatagta
       61 tatgttgaaa atgcatggat tttccaggtg tgttaccagt aagaagcagg tggataaagg
      121 atgtgataac aaatccaaca accaggttaa tatctgaaga tttttgttgt tattatttat
      181 ccccaaagcg gtgagttatt aatgaagttt gcctgaatgt ataaacagaa ttattcgaca
      241 taaagccagg aaaatgagtg atacgctttt aaccgaaaaa atccttaccg gagaaaatgt
      301 tttgcgtgct gcaatcgccc gcattgagtg gatttttgaa acatttccgt cggtatgtct
      361 gtctttttca ggtggaaagg attccaccgt gcttttccat cttgtggcag aggtggcccg
      421 caggagaaaa cgtcatttct ctgttctgtt cattgactgg gaggcccagt atcggtgcac
      481 cattgaacat attcagaaga tgcgggaaat gtaccatgat gtgacggaaa ccttttactg
      541 ggtggcactt cccctgacca cggtaaacgg cgtctcgcag tttcagccgg agtggatatg
      601 ctgggagcca ggagtgacct gggttcgcca gccaccggaa gaggccatta cggatatgac
      661 atattttccc tttttccggt atgccatgac gtttgaagaa tttgttccgg cattttcttc
      721 ctggtttgcc ggtaaccggt gtggagtggc agtgctgacc ggtgttcgtg cggatgaatc
      781 cctcaatcgc tttatggggc tggtgtctca gcgcaaactg agatatgccg atgataagcc
      841 ctggaccacg gcatcgcctg aagggtttta ttacaccatg tatccgttgt atgactggaa
      901 agcccgcgat atctggatat ataacgccag agcttgtgcc atttacaatc ctctgtatga
      961 cctgatgtac cgtgccgacg tgccgttacg caacatgcgt gtctgtgaac cttttggtcc
     1021 tgaacaacgt aaggggctgt ggctttacca tgttctggag ccggaaacct gggccaggat
     1081 gtgtgagcgg gtgtcaggcg ctgccagcgg ggcgctttat gccaatgaaa gcggtgccta
     1141 ttttgccctg cgtaaacgta tatcaaaacc agcccatcat acctggcgca gctatgcgat
     1201 gttcctgctg gatgtgatgc cggaaagaac ggcagaacat taccgtaata aaattgctgt
     1261 ctacctgcgc tggtatcaga cgcggggctt cccggatgac atcccggatg aacaggagaa
     1321 tgacctgggg agccgggata ttccgtcctg gcggcgtatc tgtaagacac ttataaagaa
     1381 tgatttctgg taccggaccc tctccttcag tccgaacaaa ccccggcact atgaacgtta
     1441 tctgcagcgt atgaaagaaa ggaggaagga atgggggatt ctgtgacacc ggagataatt
     1501 ggcaacatga ttcgtcagta cttcagccag gaacgaccag aagaagagac catacaggcc
     1561 cttaatcacc tgcgcagagt gcttcatgaa gtcagtcctt tttcacatga gccagtggac
     1621 tgtgtgctgt gggtaaaagc agatgaggtg gtggccaatg attacaatcc taatgtcatg
     1681 gcgccgggtg agaagaaact gctgaagcag tcactggaga aagatggttt cacacaaccg
     1741 gtggtggtgt ctgaggaaaa gaaccattac ctggtggtgg atggttttca tcgccaggtg
     1801 ctgggcaggg aagcaggtac gggaaaacgt ctgaagggct ggctgccggt ggtctgtatt
     1861 aatccggaga gaaagggaca ggcctcacgt attgctgcga ccatccgaca taaccgggcc
     1921 aggggcaaac atcagataac ctcaatgtct gatattgtca gggacctgtc gcgcctgggc
     1981 tggacgaatg aacgcattgg tacggagctg ggtatggacc aggatgaagt tctcagattg
     2041 aaacagatta gcgggctgac ggagctgttt caggaagagg atttcagccc ggcatggacg
     2101 gttcgctgag accgtcagat atgtatatta tttcagtgag ttaatgtttt gagcaggaaa
     2161 aagagtaccc cggaagttgt ggaagacatg gttgcgcaga aactggaggc tgctggttgc
     2221 tggcgcagag cttctgaccg ctggcttttt gttatgggaa atgttgaatg tacagaagca
     2281 caacgggagt ggttgttgtt gcgccggaat tattgtctgg cgcagatatc gtccccgccg
     2341 ctacctgaaa cgctggatat cagcgaggtg gcgaaagctg cagatgccac gctgaggcga
     2401 atggggattg ccactccgtc cggggaggta ttcagaaaag gtacaccggt ctgttgatat
     2461 tcaggtaaca ggtattaatg atgaagcaag gaatggcaaa cagtatatca atgacatggc
     2521 aacttttatg cgatgaacat ggttttaccc gggagcacta caatcagctg aagaagttca
     2581 gcccggagac attacgtgaa attattgctg aaatagccag ctgtcatcca tcaaccagtg
     2641 tattgctcag gaataagtgg ctgacaccgc cggaggatat tctggagcag attacccgtg
     2701 aatacgaacg aagaatacaa aactgtccac cgttcaggag cgagaaggaa gcggaatcct
     2761 ggttaaatga actatctttt gctgtgattg ctccgctata ccgtgttgcc cccgaacaaa
     2821 cagaacaggt tgagtccttt gttctgcagc ttattgccga gcaggagcgg gtttgggagc
     2881 ttattttaac caatgatggt tattcatggc attgcgcact ttatgatata ttatttcatc
     2941 tggttttacg aaatatggaa gaccagcctg aacatcagaa acagcgaata acaaaaatat
     3001 tttatgagcc cgatgcaaga ctggttgcag aagagattaa attccggatt caatctctgt
     3061 atgatgatga acaaaagaga gctttaagtg aactggtaaa tgattttact tca
//
LOCUS       DAZUGK010000317         3099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZUGK010000317 DAZUGK010000000
VERSION     DAZUGK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.319"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..1740
                     /gene="ydeN"
                     /locus_tag="ACHY5W_004609"
     CDS             58..1740
                     /gene="ydeN"
                     /locus_tag="ACHY5W_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4449347.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1792..2949
                     /gene="ydeM"
                     /locus_tag="ACHY5W_004610"
     CDS             1792..2949
                     /gene="ydeM"
                     /locus_tag="ACHY5W_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS4449348.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2915..3037)
                     /locus_tag="ACHY5W_004611"
     CDS             complement(2915..3037)
                     /locus_tag="ACHY5W_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449349.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          940 a          672 c          633 g          854 t
ORIGIN      
        1 cttatttctc ttcgtaaaat tactttggaa ttaagtacaa taagaagagg aacatttatg
       61 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
      121 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
      181 gttgctttct cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc
      241 gtactgacca tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
      301 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
      361 attgaagctg cgcaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgt
      421 tttactaacg gctatgtggc gcacggtgtt tccggcccct cccgcgccgc aataatgacc
      481 ggtcgagcgc ccgcccgctt tggtgtctat tccaatactg atgctcagga tggtattccg
      541 ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta
      601 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacacgt
      661 gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc
      721 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
      781 ttcaaaaatc gtgaacgcgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
      841 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct
      901 tataatgctc cgcacctgcc aaatgataat cctgcaccgg agcaatatca gaagcaattt
      961 aataccggta gtcaaacagc agataactac tacgcttccg tttactctgt tgatcagggt
     1021 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     1081 tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     1141 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     1201 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca
     1261 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg
     1321 ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc
     1381 tcttattctc actggtttga tgaggaaaat attccattct gggataacta ccacaaattt
     1441 gtccgtcatc agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc
     1501 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta
     1561 ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     1621 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt
     1681 agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa
     1741 ctaaaccttc atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt
     1801 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     1861 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc
     1921 aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     1981 actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc
     2041 caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt
     2101 gcctttctca aagaacatga gtttctggtt ggtatctcga tcgatggacc ccaggagcta
     2161 catgaccgtt acagacgcag taattcaggt aacggtactt ttacaaaagt gatagcagcc
     2221 atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat taataacgtt
     2281 aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg
     2341 caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg tcatagtgag
     2401 aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca
     2461 accattttta tgcaatgggt taaaaacgat gtgggtgaag ttttcatccg tcagtttgaa
     2521 agctttgtca gccgtttttt ggggaatggg cataccagtt gtattttcca ggagtcctgc
     2581 aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat
     2641 ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa
     2701 ctgacagcgc aaaaaaaacg gatttcagcg aaatgtctga aatgtgcata taaacctatc
     2761 tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat
     2821 ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa cgccatggta
     2881 gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag
     2941 aataattaat aacgctctct tttagctcca ttcttagcct ccttcttatg accttaacaa
     3001 ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc
     3061 ctggcttacg tggtatgcgt cagggcatta gcaataccc
//
LOCUS       DAZUGK010000318         3088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZUGK010000318 DAZUGK010000000
VERSION     DAZUGK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.320"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(254..1732)
                     /locus_tag="ACHY5W_004612"
     CDS             complement(254..1732)
                     /locus_tag="ACHY5W_004612"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS4449350.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKENIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1759..3036)
                     /gene="yqcE"
                     /locus_tag="ACHY5W_004613"
     CDS             complement(1759..3036)
                     /gene="yqcE"
                     /locus_tag="ACHY5W_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFS4449351.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAIASSK"
BASE COUNT          824 a          791 c          699 g          774 t
ORIGIN      
        1 tacataaccg cctctatacg ctgttgatga atagttctta tgaataaaga tatccagttc
       61 atactttaag tgaaaattga taaagtgcga ttcgtattgt cttttattct aaagacatcg
      121 agtgtagtta atattccttg taaaaacagg gataaaccga actagttaaa gtttttatta
      181 tgttccccgc gtcagcgggg ataaccattc aggtatgttc aggccgggct gtttcccgcc
      241 cggtcttcct cacttacgca ttcgaccagc tctggatcga atccgcattc cccaattccc
      301 catgcatcac ttcatacgat tttttcagga tcacatcagt atgtttggtt aggtctttga
      361 aaataccttt attcatcgcg tcgtagcgtt tggcattgct ctcaacaggc ataaagatat
      421 ctttcacgcg aaccattttg tcgacagccg ttgcgtaatc cgggtatagc cccagaccta
      481 ccgccgtatt gatcgctgcc cccagacttg cacaaccgtt aatggcgtta cggcgtgccg
      541 gaaggttgaa cacgtcggca aaaatttgca taaacagatc gctgttcgaa ccgccgccag
      601 taatgatcac atgctttgca aagtgattca tttcattaca catattgtcg taattgttct
      661 tcagcgtcag cgccacgctt tccagtatcg aacgatatat ccatgcgtaa tccatgctgg
      721 aatcaaagcc gatcataatc ccacgtttgt agggttccca cgggttggtc agccagtcca
      781 gcaccgtcat cagaccatta cagcctggcg gcacacaaga agcttttttg ttgagtaaat
      841 cttccggtga aagatcctgc gccctggcat cctgaattaa cgactcgcct agcatgtcgc
      901 gcagccagct caccgtccac atgcctttgc gaataccgta accttcatac agcaatgttt
      961 gcggaataga agacataatc ggccagtacg ccaccggatc tttcggcagt gctttaccgt
     1021 tcatcatcag cgcgatgtag gtgccaagag aaatcaccgc cgtttcatca tccagtaatc
     1081 cggctcccag agcttctacc ggtttatcac tggtggtaca aacaaccggc agccctgccg
     1141 ggaaatgtgt cgccagcgcg gcttgaggtg tgatctgtcc aagaacagtg cctggcattt
     1201 gcacatcaaa cagcatatga cgggggatat taaacttctc catgactgcg gcatcttcac
     1261 tccatgccca gctcttataa tccaccggcc actgaccaaa atagttggcg atattgtctt
     1321 taaactcgcc ggttaagcga tgcgtcagat aacccgaaaa agaggtgaca taggccacgt
     1381 caggattagt gtgttcgtag ggacgcgtta cgcgtgcatc ctgccagcta atcaatggcg
     1441 cagctggcgt gccatcggct ttcaataacg cacggcagca acggatggat cccagaccaa
     1501 taccgacaat attttcttta ttcccggcaa actgactcat caaatcgtga ccggcaaaac
     1561 ataatgatgc ccataaatcg tcgtcaggat gttctgcggt atcggcatct ggcgtgtgca
     1621 tcggctgcaa taagccttta ccttcgcaaa ccacattacc ttccagatcg tacatcacca
     1681 cttttgtgct ctgacttccg ccatcaatcc ctatgatgta ttttttcgac atgatttttc
     1741 tcctgtaatt ttcctggctt acttgctgct agctattgcg ggggcgctat cagcagtacg
     1801 aattttttgg aacagcaaaa aggtaaagac aatcaccatg gcaagcgccg ccatgcccat
     1861 cagccacata ttgcggtagg cttctgctgc gggcagggtg tcttgccagt ggccgataat
     1921 ggggtaaacg aagacatccg gcaggaatcc aatcaccgaa caaataccta ccgtggtgcc
     1981 cataatgtaa gacggtgttc ttgcttcgcc agggcaggcc cagtacagcc cgcgtgaggc
     2041 gtaacaggtg aatcccagca gtaaaatcag gccaatcccc atcgcgaccg attgcgggtt
     2101 agagttcgtg acgagcaggg cagttaacgc cagcaggccg agtacggaaa ggatttggat
     2161 cacgcgggta ggggatttca ctttactgta ggtggtgata atgccgccaa gtgggccgca
     2221 cagcgcgcgg aagattttgt tgatcacaat ccccatgtag ctcgccgcca ccagcgacat
     2281 gccatacatt tcggtcagat agttggtgga gtaactcaga atggcgtaga tggtgaagac
     2341 accaaaaatc accatgctgc aataccaggt ggtgctgatg cgcaaaacgg ccaggatgtc
     2401 gctaagctgg aatgattgtt tttcttcgtt attggcactg cgcaggttgt tgttatcgct
     2461 aacaaaaaac cagcacagaa tccccaacaa gatgtaaacc acactgtaga taattatgac
     2521 cgtttttagg ctggcgctgt catccggtgc aaagcgagaa aagacccaca tggtaaacac
     2581 cgccagcgac attacaccga cgccgcgcag cccttccatc cagcccataa ttttcccttg
     2641 ctcgctatga tcgccaagca acgatgcggc tttaatcgac accgaccaca gcattaaaat
     2701 cgtcgttatc gcaaatgcga cctgaataca gagcattacc cacagcggtg gatacgttgc
     2761 cattaacaga cccagtaatc cggtaatgat catcgcggaa gtaatcattt tgcgatgtga
     2821 gaatttatcg gcaataacgc cgctgggggc ataaagaata atagctgcaa taccaaaggt
     2881 actcattatt aaacctatct cggtattgct gaatcccata aatttcgcca tgggaatttg
     2941 ataaatataa cgtaaataag ccaggtcgaa actaacgccg ccgctaaaac taattatcgc
     3001 gagggttatc caacggcgat atgagttgtg ttgcatatcg tattcccatt tgtaatagac
     3061 aaaaaaaaga gaaaagtaag ccgccctt
//
LOCUS       DAZUGK010000319         3030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZUGK010000319 DAZUGK010000000
VERSION     DAZUGK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.321"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..831
                     /locus_tag="ACHY5W_004614"
     CDS             <1..831
                     /locus_tag="ACHY5W_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4449352.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGK
                     EWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
     gene            883..1257
                     /gene="gpFI"
                     /locus_tag="ACHY5W_004615"
     CDS             883..1257
                     /gene="gpFI"
                     /locus_tag="ACHY5W_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4449353.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            1250..1603
                     /locus_tag="ACHY5W_004616"
     CDS             1250..1603
                     /locus_tag="ACHY5W_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4449354.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGGEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1619..2152
                     /locus_tag="ACHY5W_004617"
     CDS             1619..2152
                     /locus_tag="ACHY5W_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449355.1"
                     /translation="MAIKGLEQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVVGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            2149..2544
                     /locus_tag="ACHY5W_004618"
     CDS             2149..2544
                     /locus_tag="ACHY5W_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4449356.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            2552..>3030
                     /locus_tag="ACHY5W_004619"
     CDS             2552..>3030
                     /locus_tag="ACHY5W_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFS4449357.1"
                     /translation="MSTPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     "
BASE COUNT          719 a          673 c          956 g          682 t
ORIGIN      
        1 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac
       61 cagcaggcgg ttgagcgcct tcctggtgaa gatccggctc aactgaacga tccggcttac
      121 cgccgtctgc gtatcattac cgataacctc aaacaggaag agcacgcgat tgtccaggtg
      181 gaagaaatac aggcggtaaa tgctgtgttg tatggcaaat atacgatgga aggagaccag
      241 ttcgagaaaa ttgaggtcga ttttggcagg tcgacgaaga ataacatcac tcagggtagt
      301 ggtaaggagt ggtcaaaaca ggatcgtgac acgttcgatc ctacacatga tcttgacctc
      361 tactgcgacc aggccagcgg tcttgtgaat attgccatta tggacggtac cgtctggcgt
      421 cttctgaatg gttttaagct gttccgcgaa aaactggata cccgtcgcgg ttcaaattct
      481 caactcgaaa cggcagtgaa agatctgggc gcagtggtgt ccttcaaggg gtattacggc
      541 gatctggcca ttgtggtggc gaaaacgtct tatatagcag aagacggtat cgaaaaacgt
      601 tatctgccgg agggcatgct ggttctgggg aatactgctg cagatgggat ccgttgttac
      661 ggtgccattc aggatgcgca ggcgttgtcc gaaggtgtgg tggcctcttc ccgttatccg
      721 aaacactggc tgacggtggg ggatcccgcc cgtgaattta ccatgacgca gtccgcgccg
      781 ctgatggtgt tgccggaccc ggatgagttt gtggtggtac aggtgaaata atccgtgagc
      841 gggggcgaaa tgcccccgtg tcttttttca cagggggctg atatggcaac gaaagagcaa
      901 aatctgaaac ggcttgatga actggccctg attctggggc gtgagccgga tatatccggg
      961 agtgccgcag agatagcgca gcgggtggcg gaatgggaag aggaaataca gtcatccggc
     1021 gaggatgttc aggttgggga tacggtgatc cgggagcggg aaaccgcggc tcatgatgtt
     1081 cgtgaggata catccggtgc gttaacgcgc atcagagttc tgacctgcct ccatctctgt
     1141 ggcattgatg gtgaaacagg ggaatccgtt gagattgcgg atgttggtcg ggtgattctg
     1201 attatgtcct cagatgcaaa aacacacgtt gatggcggaa tggctgttta tgcgtgattt
     1261 tcagaatgcc tttgatgctg cccttgccgg ggtggacagt acgattgttg aagtgatggg
     1321 gctctgtgca cagttcacct cgggggcaca gtgtggcgga gaagttcagg gggtttttga
     1381 cgatccggag tcgctgggat ttgccggtag cggggtccgt attgaaggaa gcagcccgtc
     1441 attatttgtg cggacggata cggttcgtgc tgtgcggcgt ggtgacacgc tgaccattaa
     1501 tggtgagacg ttctgggtgg atcgtgtttc tccggatgac gggggcagct gttatctctg
     1561 gctcaaccgt gggcaaccac ccgccgttaa ccggcgacga taaacgcagg gtgaaattat
     1621 ggcgataaaa gggcttgagc aggcgattga taatctgagc cgggttcgta aaaacgccat
     1681 tccggcggct tctgcaatga ccattaaccg cgtggccaca acggcgatta atcagtcttc
     1741 atcacaggtt gcccgggaaa ccagggtgag acggaaactg gttaaggaac ggtccagact
     1801 gaaacgggcc acggtcagaa atccgaatgc cagaattatc gttaaccgcg gtgatctccc
     1861 ggtgattaag ctggggatca ggatgccggg gcgtcgtccg gacagcatac tcaaagccgg
     1921 tcagcatcgt tatcagcggg catttattca gcgattaaaa aatggtcgct ggcatgtcat
     1981 gcagcgtgtg gtcgggaaaa accgttaccc cattgatgtg gtgaaaatcc cgatggcggc
     2041 cccactgaaa caggcgtttg atgagaatgt tgaccgtatc cggcgtgaac gtctgcccgg
     2101 agaactggca tacgcgctga aacaacaact gaggattgcg ataaaacgat gaaacatact
     2161 gatatccgtg ctgcagtgct ggatgcactg gagctgcatg aacacggggc gacgctgttt
     2221 gatggtcgcc ccgttgtttt tgacgaagag gattttcccg cggtcgcggt ttatctgacg
     2281 gatgcagagt ataccggtga agagctggat gcagatacct ggcgggccac actgcatatt
     2341 gaggtgtttt taccagcaca ggtaccggat tcggagctgg attcgtggat ggaaagccgg
     2401 atttatccgg cgatgactgc gatcccggca ctggcagacc tgattaccac gatggttacg
     2461 cagggctatg agtatcgtcg tgatgacgat atggcgttat ggagttctgc ggatctgact
     2521 tattccatta catacgagat gtgaggacga tatgtcaaca ccaaatcccc ttgagccggt
     2581 aaaaggtgcc ggtaccaccc tgtgggttta taacggtcag ggtgacgcct atgcaaaccc
     2641 gttgtcagac gatgactggc tgcgactggc taaggtgaag gatctgacgc cgggcgagat
     2701 gacggcagaa ccctacgatg ataactacct ggatgatgaa gacgcggact ggaccgcgac
     2761 cgggcagggg cagaagtctg caggagatac cagttttacg ctggcctgga aaccgggaga
     2821 agaaggtcag aaagggctta taggctggtt tgaaagcggg gatgtgcggg cctataaaat
     2881 ccgttttccg aacggcacgg tggatgtgtt tcgtggctgg gtcagcagta tcggtaaggc
     2941 cgtgacggcg aaagaagtga tcacccgcac ggtgaaagtg accaacgtgg gcaaaccttc
     3001 tgtagcggaa gaacgcagca aaattacgcc
//
LOCUS       DAZUGK010000320         3005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZUGK010000320 DAZUGK010000000
VERSION     DAZUGK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.322"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(145..221)
                     /locus_tag="ACHY5W_004620"
     tRNA            complement(145..221)
                     /locus_tag="ACHY5W_004620"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Asp,seq:gtc)
     gene            complement(354..1085)
                     /gene="dnaQ"
                     /locus_tag="ACHY5W_004621"
     CDS             complement(354..1085)
                     /gene="dnaQ"
                     /locus_tag="ACHY5W_004621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFS4449358.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1150..1617
                     /gene="rnhA"
                     /locus_tag="ACHY5W_004622"
     CDS             1150..1617
                     /gene="rnhA"
                     /locus_tag="ACHY5W_004622"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFS4449359.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1614..2336)
                     /locus_tag="ACHY5W_004623"
     CDS             complement(1614..2336)
                     /locus_tag="ACHY5W_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4449360.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2370..>3005
                     /gene="gloB"
                     /locus_tag="ACHY5W_004624"
     CDS             2370..>3005
                     /gene="gloB"
                     /locus_tag="ACHY5W_004624"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFS4449361.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTHVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTSSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQ"
BASE COUNT          809 a          726 c          708 g          762 t
ORIGIN      
        1 ttaatgatat ggtctaccat atgtgattaa cacatttaca gcataacaaa atcatagacc
       61 gaagaaatag cgggatttaa atgcaaaaaa ccgcaaaatt aaaaatcttg cggttctctg
      121 aacctcattt tcatgaggtg aatttggcgg aacggacggg actcgaaccc gcgaccccct
      181 gcgtgacagg caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga
      241 acgaggcgaa tattacggat tgcctcaccc gtcgtcaatg gtttttctca tcttttgaat
      301 cgtttgctgc aaaaatcgcc caagtcgcta tttttagcgc ctttcacagg tatttatgct
      361 cgccagaggc agcttccgcc tttcttctgc accagatcga gacgggcttc atgtgctgca
      421 agctcttcat ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga
      481 attgttgctt caccttgttg ctgttgtgtc tctccttcca tcgcaaaagc catcgacgtt
      541 tgaccaccgg tcatcgccag ataaacttcc gcaaggatct gggcatcgag taatgccccg
      601 tgcagcgttc gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg
      661 cgtttaccgg gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta
      721 ttagtcttcg gaatatcgcg cttaagcaac gagaactcgt agtccataaa gccgatatcg
      781 aacgctgcgt tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct
      841 acttcggcaa acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag
      901 gcttccggat ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg
      961 cgacggttca ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca
     1021 ccaatctggt tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg
     1081 ctcatagcgg tcatttatgt cagacttgtc attttacagt tcgattcaat tacaggaagt
     1141 ctaccagaga tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatccc
     1201 ggacctgggg gttatggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgct
     1261 ggctacaccc gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag
     1321 gcgttaaaag aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggt
     1381 atcacccagt ggatccataa ctggaaaaaa cgtggctgga aaacagcaga caaaaaacca
     1441 gttaaaaatg tcgatctctg gcaacgtctt gatgctgcgc tggggcagca tcaaatcaaa
     1501 tgggaatggg ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggct
     1561 cgtgccgcgg cgatgaatcc cacactggaa gatacgggct accaagtcga agtttaagcc
     1621 tgtggtttac gacattgccg ggtggctcca accgcctggc gaattcgtgg cttgttttta
     1681 ctctgtttca tcgggtttag cgttaaagga atagtccgtt tccgggcaac aataagttgt
     1741 aagcaaccaa gcgcaggaat atgcgcattc aaaagttttc ctccgtgttt gttccacggg
     1801 agaacgtgga aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt
     1861 tgccgcatca gagtaaacat ccggctgtta tagggcgagg tttttcgcaa taccggcaca
     1921 agtttgcgta atcccattaa actaatggga ttgaagccac taatcaccag ccagccatca
     1981 tcaatcaata cccgatcggc ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat
     2041 gtatgtgcca gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtggatcc
     2101 gcctgcacct ggacgggcat tccttgagca gaaacattca cttgatgaga aaccgcgcac
     2161 gcttcgcaat tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt
     2221 ttcgtgaacc atgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc
     2281 aaatcgcccc agcaatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca
     2341 accttccgtt tcacactgag aggtaatcta tgaatcttaa cagtattccc gcctttgatg
     2401 acaattacat ctgggttttg aatgatgagg caggtcgctg cctgattgtc gatcccggag
     2461 acgcagagcc agtattaaac gccattaccg ccaataactg gcaaccggag gccatatttc
     2521 tcacccacca tcaccacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc
     2581 aaattgtggt gtatggtcca caagagacac aagataaggg aacaacacat gtagtcaaag
     2641 atggcgaaac tgccttcgtt ttggggcatg aatttagtgt aattgccacg ccgggtcaca
     2701 ctttaggaca tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt
     2761 ctggtgggtg tggtcggttg tttgaaggga catcatcaca aatgtatcaa tcacttaaaa
     2821 agttaagtgc gttacctgac gatacattgg tatgttgtgc tcatgaatat accttatcaa
     2881 atatgaagtt tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta
     2941 aagttaagga gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc
     3001 ggcaa
//
LOCUS       DAZUGK010000321         2991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZUGK010000321 DAZUGK010000000
VERSION     DAZUGK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.323"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..269
                     /locus_tag="ACHY5W_004625"
     CDS             <1..269
                     /locus_tag="ACHY5W_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFS4449362.1"
                     /translation="HYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAGLLVIEGKV
                     WRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            343..783
                     /locus_tag="ACHY5W_004626"
     CDS             343..783
                     /locus_tag="ACHY5W_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFS4449363.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            780..1307
                     /locus_tag="ACHY5W_004627"
     CDS             780..1307
                     /locus_tag="ACHY5W_004627"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFS4449364.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAA"
     gene            1304..1486
                     /locus_tag="ACHY5W_004628"
     CDS             1304..1486
                     /locus_tag="ACHY5W_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS4449365.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            1483..1653
                     /locus_tag="ACHY5W_004629"
     CDS             1483..1653
                     /locus_tag="ACHY5W_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFS4449366.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            1646..2257
                     /locus_tag="ACHY5W_004630"
     CDS             1646..2257
                     /locus_tag="ACHY5W_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFS4449367.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            2254..2919
                     /locus_tag="ACHY5W_004631"
     CDS             2254..2919
                     /locus_tag="ACHY5W_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFS4449368.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2916..>2991
                     /locus_tag="ACHY5W_004632"
     CDS             2916..>2991
                     /locus_tag="ACHY5W_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449369.1"
                     /translation="MRLESVAKFHSPKSPMMSDSLLAIV"
BASE COUNT          895 a          669 c          801 g          626 t
ORIGIN      
        1 ttcattacct ggggacgcat aatagcttct gtgcgccgga cgttgccgcg ttaacaggcg
       61 caacagtaac cagcataaat caggccgcgg ctaaaatggc acgggcaggt cttctggtta
      121 tcgaaggtaa ggtctggcga acggtgtatt accggttcgc tactagggaa gaacgggaag
      181 gaaagatgag cacgaacctg atttttaagg agtgtcgcca gagtgccgcg atgaaacggg
      241 tattggcggt atatggagtt aaaagatgac catctacatc actgagctaa taacaggcct
      301 gctggtaatc gcaggccttt ttatttgggg gagagggaag tcatgaaaaa actaaccttt
      361 gaaattcgat ctccagcaca tcagcaaaac gctattcacg cagtacagca aatccttcca
      421 gacccaacca aaccaatcgt agtaaccatt caggaacgca accgcagctt agaccaaaac
      481 aggaagctat gggcctgctt aggtgacgtc tctcgtcagg ttgaatggca tggtcgctgg
      541 ctggatgcag aaagctggaa gtgtgtgttt accgcagcat taaagcagca ggatgttgtt
      601 cctaaccttg ccgggaatgg ctttgtggta ataggccagc caaccagcag gatgcgtgta
      661 agcgaatttg cggagctatt agagcttata caggcattcg gtacagagcg tggcgttaag
      721 tggtcagacg aagcgcgact ggctctcgaa tggaaagcgc gatggggaga tcgggctgca
      781 tgactatcaa atcaaatacg ccagcacacg acaaggactg ctggcaaacg ccgctttggc
      841 tttttgatgc actggatatt gagtttggat tctggctgga ttcggcagcg agcgacaaaa
      901 acgctctgtg cgctcactgg ctaactgagg ctgacgacgc gctaaattct gagtggataa
      961 gccacggtgc aatctggaat aacccaccgt acagcaatat caggccgtgg gtggaaaaag
     1021 ccgctgagca gtgcatacaa cagcgacaga cggtagtgat gcttgtgcca gaggatatgt
     1081 ctgtcggatg gttcagcaag gctctggaga gtgttgacga agttcgtatt atcactgatg
     1141 gacggattaa ttttatcgaa ccatcgacag ggctggagaa gaagggaaac agcaaaggct
     1201 ccatgctgct gatttggcga ccgttcatta gtcctcgacg gatgtttact atcgtatcca
     1261 aagcggcatt gatggcgatc gggcagggcg tcaggagggc ggcatgaggc gacaacgacg
     1321 aagtttcacc gacatcatct gcgaaaactg caaatacctt ccaacgaaac gctccagaaa
     1381 taaacgcaag ccaatcccaa aagaatctga cgtaaaaacc ttcaactaca cggctcacct
     1441 gtgggatatc cggtggctaa gacatcgtgc gaggaataca aggtgattga cccaaatcga
     1501 agttacgaac aagaaagcgt cgagcgggct ttaacgtgcg ctaattgcgg tcagaagctg
     1561 catgtgctgg aagttcacgt gtgtgagcac tgctgtgcag agctgatgag cgattcgaat
     1621 agctcgatgc acgaggaaga agatgatggc taaaccagcg cgaagacgat gtaaaaacga
     1681 tgaatgtcgg gaatggtttc accctgcatt cgctaatcag tggtggtgct ctccagagtg
     1741 tggaaccaag atagcactcg aacgacgaag taaagaacgc gaaaaagcgg aaaaagcagc
     1801 agagaagaaa cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcgaaaact
     1861 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagccgtaa acgccttcat
     1921 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
     1981 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
     2041 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
     2101 cgtcgaactg attagccgca tcgggcagga agcagtagac gaaatcgaat caaaccataa
     2161 ccgccatcgc tggactgtcg aagagtgcaa ggcgatcaag gcagagtacc aacagaaact
     2221 caaagacctg cgaaacagca gaagtgaggc tgcatgaata tctacgaaag aattgatggc
     2281 agcaaatacc gaaatatttg ggtagctggc gacctgcacg gatgctacac gaacctgatg
     2341 aacaaactgg atacgattgg attcgacacc aaaaaagacc tgctcatctc ggttggcgat
     2401 ttggttgatc gcggtacaga gaacgtcgaa tgtctggaat taatcacatt cccctggttc
     2461 agagctgtgc gtggaaacca tgagcaaatg atgattgatg gcttatcaga gcgtggaaac
     2521 gttaatcact ggctgcttaa tggcggtggc tggttcttta atctcgatta cgacaaagag
     2581 actctggcta aagttcttgc ccataaagca gaagaacttc cgttaatcat cgaactggtg
     2641 agcaaagata aaaaatatgt catttgccac gccgattatc cttgtgataa atacgagttt
     2701 ggaaagccag ttgatcatca gcaggtaatc tggaaccgcg aacgaatcag caactcacaa
     2761 gacgggatcg tgaaagaaat caaaggcgcg gacacgttca tctttggtca tacgccagca
     2821 gtgaaaccac tcaaatttgc caaccagatg tatatcgata ctggcgcagt gttctgcgga
     2881 aacctcacat tgattcaggt acagggagaa ggcgcatgag actcgaaagc gtagctaaat
     2941 ttcattcgcc aaaaagcccg atgatgagcg actcactact ggccatagtt t
//
LOCUS       DAZUGK010000322         2971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZUGK010000322 DAZUGK010000000
VERSION     DAZUGK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.324"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1833
                     /gene="recE"
                     /locus_tag="ACHY5W_004633"
     CDS             <1..1833
                     /gene="recE"
                     /locus_tag="ACHY5W_004633"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFS4449370.1"
                     /translation="LARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGG
                     LDYSRAIVVASVKEAPIGIEVTPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPM
                     PQRVTEEEEKQDDEEKLQPSCAMADEQATAETVEPDATEHHQDTQSLDAQSQVNSVDA
                     KYQKLRAELHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSVY
                     QNQPETEHNDQKAEQNDPNTQQNEPETEQPEPVVQQQETEKVCIACGQAGGGNCPDCG
                     AVMGDATYQKTFNEENQNEYQEKGLEEMEGAEHPHKENDGSDPHRDCSDETGEVADPV
                     IVEDIEPGIYYGISNENYHVGPGVSKSQLDDIADTPALYLWRKNAPVDNTKTKTLDLG
                     TAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGLKIE
                     LMYQSVMALPLGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTA
                     DIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGE
                     EAKLAGQLEYHRNLRTLADCLNTNEWPAIKTLSLPRWAKEYAND"
     gene            1826..2635
                     /gene="recT"
                     /locus_tag="ACHY5W_004634"
     CDS             1826..2635
                     /gene="recT"
                     /locus_tag="ACHY5W_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFS4449371.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            2692..2886
                     /gene="ralR"
                     /locus_tag="ACHY5W_004635"
     CDS             2692..2886
                     /gene="ralR"
                     /locus_tag="ACHY5W_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFS4449372.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTENINGGVHV"
     gene            2807..>2971
                     /locus_tag="ACHY5W_004636"
     CDS             2807..>2971
                     /locus_tag="ACHY5W_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS4449373.1"
                     /translation="MVEVKKKRSKDGINEQLKILMEAFMYKITATIEKEGGTPTNWTR
                     YSKSKLTKSEC"
BASE COUNT          923 a          673 c          729 g          646 t
ORIGIN      
        1 ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc taaacgtgtc
       61 gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa attcatcacc
      121 atgcctggcg ggctggatta ttcccgcgcc atcgtggttg cgtccgtaaa agaagcacca
      181 attgggatcg aggttacccc cgcacacgtc actgaatatc tgaacaaagt actgactgaa
      241 actgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg ttcctctgcc
      301 ccgatgccgc agcgtgtaac agaagaagaa gaaaaacagg atgatgaaga aaaactgcaa
      361 ccatcttgcg caatggcaga tgaacaggca acggctgaaa cagtggaacc ggatgcaact
      421 gaacatcatc aggacacgca gtcgctggat gctcagtcac aggtaaattc tgttgatgcg
      481 aaatatcaga aactgcgggc agaactccat gaagcccgga aaaacattcc gcccaaaaat
      541 cctgtcgatg cagacaaatt actggctgcc tctcgcggag aatttgttga agggattagc
      601 gacccgaatg atccgaaatg ggttaagggg atccagaccc gcgactctgt gtaccagaat
      661 cagccagaaa cggaacataa cgaccagaaa gcggaacaga acgacccaaa tacgcaacaa
      721 aacgagccag aaacggaaca gcctgagcca gtagtgcaac aacaggaaac ggagaaagtt
      781 tgcatcgcct gcggtcaggc tggcggcggc aactgtcctg actgcggcgc ggtgatgggc
      841 gacgcaacgt atcagaaaac ctttaatgaa gaaaatcaga atgaatatca ggaaaaaggt
      901 ctggaggaaa tggaaggtgc tgaacatccg cacaaggaga acgatggcag cgatccgcat
      961 cgcgattgca gtgatgaaac tggcgaagtc gcagatcccg taatcgtaga agacatagag
     1021 ccaggtattt attacggaat ttcgaatgag aattaccacg taggtcccgg tgtcagtaag
     1081 tctcagctcg atgacattgc tgatactccg gcactgtatt tgtggcgtaa aaatgccccc
     1141 gtggacaaca caaagacaaa aacgctcgat ttaggaaccg ctttccactg ccgggtactt
     1201 gaaccggaag aattcagtaa ccgctttatc gtagcacctg aatttaaccg ccgtacaaac
     1261 gccggaaaag aagaagagaa agcgtttctg atggaatgcg caagcacagg aaaaacggtt
     1321 atcactgcgg aagaaggtct gaaaattgaa ctcatgtatc aaagcgttat ggctttgccg
     1381 ctggggcaat ggcttgttga aagcgccgga cacgctgaat catcaattta ctgggaagat
     1441 cctgaaacag gaattttgtg tcggtgccgt ccggacaaaa ttatccctga atttcactgg
     1501 atcatggacg tgaaaactac ggcggatatt caacgattca aaaccgctta ttacgactac
     1561 cgctatcacg ttcaggatgc attctacagt gacggttatg aagcacagtt tggagtgcag
     1621 ccaactttcg tttttctggt tgccagcaca actattgaat gcggacgtta tccggttgaa
     1681 attttcatga tgggcgaaga agcaaaactg gcaggtcagc tggaatatca ccgcaatctg
     1741 cgaaccctgg ctgactgcct caataccaat gaatggccag ctattaagac gttatcactg
     1801 ccccgctggg ctaaggaata tgcaaatgac taagcaacca ccaatcgcaa aagccgatct
     1861 gcaaaaaact cagggaaacc gtgcaccagc agcaattaaa aataacgacg tgattagttt
     1921 tattaaccag ccatcaatga aagagcaact ggcagcagct cttccacgcc atatgacggc
     1981 tgaacgtatg atccgtatcg ccaccacaga aattcgtaaa gttccggcgt taggaaactg
     2041 tgacactatg agttttgtca gtgcaatcgt acagtgttca cagctcgggc ttgagccagg
     2101 tagcgccctc ggtcatgcat atttactgcc ttttggtaat aaaaacgaaa agagcggtaa
     2161 aaagaacgtt cagctaatca ttggctatcg cggcatgatt gatctggctc gccgttcagg
     2221 tcaaatcgcc agcctgtcag cccgtgttgt ccgtgaaggt gacgaattta atttcgaatt
     2281 tggccttgat gaaaagttaa tacaccgccc aggagaaaac gaagatgccc cggttaccca
     2341 cgtctatgct gtcgcaagac tgaaagacgg aggtactcag tttgaagtta tgacgcgcaa
     2401 acagattgag ctggtgcgca gccagagtaa ggctggtaat aacgggccat gggtaactca
     2461 ctgggaagaa atggcaaaaa aaacggctat tcgtcgcctg tttaaatacc tgcctgtctc
     2521 aattgaaatc cagcgtgcag tatcaatgga tgaaaaggaa ccactgacaa tcgatccggc
     2581 agactcctct gtattaaccg gggaatacag tgtgatcgat aattcagaag aataattcag
     2641 cctggcggtg taatgcaccg ccaacgcgag acagttttta tgacaaaaat tatgagatat
     2701 gacgatgtta aaccatgtcc gttttgtggt tgtccatctg ttacagtgaa agcaatttca
     2761 ggatattacc gagcgaagtg taacggatgc gaatcccgaa ctggctatgg tggaagtgaa
     2821 aaagaagcgc tcgaaagatg gaataaacga acaactgaaa atattaatgg aggcgttcat
     2881 gtataaaatt accgctacaa ttgaaaagga aggtggcact cctactaact ggacaagata
     2941 ttcaaaatct aaactaacga aatcagaatg c
//
LOCUS       DAZUGK010000323         2952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZUGK010000323 DAZUGK010000000
VERSION     DAZUGK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.325"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..242)
                     /locus_tag="ACHY5W_004637"
     CDS             complement(<1..242)
                     /locus_tag="ACHY5W_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFS4449374.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIDNTGNIIECADLQETTNAIKVHFLTQNALRF"
     gene            complement(255..2705)
                     /locus_tag="ACHY5W_004638"
                     /pseudo
     CDS             complement(255..2705)
                     /locus_tag="ACHY5W_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(2718..>2952)
                     /locus_tag="ACHY5W_004639"
     CDS             complement(2718..>2952)
                     /locus_tag="ACHY5W_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS4449375.1"
                     /translation="QLNIKKKNNAISINNETANWITVTTIKAQNVKVNNESILLPPFS
                     NNDITLKNNHASEYELTVVDDYGNNIHSKIAAR"
BASE COUNT          820 a          589 c          589 g          954 t
ORIGIN      
        1 ttaaacctta aagcgttctg tgttaaaaaa tgaactttaa tggcgttggt cgtctcttgc
       61 aagtctgcac actcaatgat attcccagtg ttgtcaattt tttcggcggt gagattacct
      121 ttcatactca gtggtttgag tatatatgtt ccacgttgtt ccccgacatt ggtcaatcca
      181 cagggagcac tttgcacaat aaaagaatat agactcaagg tgattaataa aagccacttc
      241 atagtaaact ccttttactg gcatatatat gttttgtttt catcgataac gtgttgaaaa
      301 gtaatagtac agaataaatt ctgttcatca ttgagagcaa cttttatgcc agttggaatt
      361 tcatctacgc gaataaaaag gcgactaccc tggccaacga ctccaatatt gttttcctgg
      421 agatccagta cgtcatatcc aaaggttaat ggcgaaccat cgggacgcag tgcctgtata
      481 taccagggct tacgttggtc agttgtaaac tgcacataag aaactgctcc acggtaaggc
      541 gcaataattt gacgattgcc ctgcaattct gtttcacttt cactgtttgc tatatcaagt
      601 accagatggt tttcacgatg aggtataatc aggtcgtaaa caacctggcc tttgctattt
      661 gttcggttgt atttttgtcc attaatattc gcgccttcta acccaggcgc atccagaatt
      721 gcaaatgtat cggatagctg gttagtgata ttaataccac cgggccagac aactaatcca
      781 ctactaatac ttccaccact ttgccaggcg tttttagaat ggctatagct gccatctatt
      841 gtggcgattg gagttctcca gcttaaattc gtaccaagcg aggtatcatt attttgttgt
      901 tgctgattaa catatatacc ataatttaac tgatcatgtt caccggctat gccagtaatt
      961 ccagtattgt tggaggaata gccatctttg gaaaatgagg tagaattcga taagttaatt
     1021 tggtgacgtg ttttggcaat atcatcaccc cagtagaaag gaatggagat aaacagatta
     1081 aaacgctcct cttctttatc gttttcatca taggattggc tggcagagaa agtataacta
     1141 atgtgttgcc agttattgga gtaactgaat tggtaatctt tagcatttcc acttcgcccc
     1201 cagtaattcc gccaaagtgc acttaatgat acatttccca gattattgga taaaggttgc
     1261 attatattgg cagaaaggct attttttctg ccaatatcat aaaaatcatc atagtcactc
     1321 tgatgattta ttttatcatt ttcgtaaaga tggtcgctga atgtcctgta atcctgcgaa
     1381 gcataacgcc aggcggccac gctaaagtga gtggccgttt gcaatagata tttattatag
     1441 gcgacctgat agctcgtacc ttcgtgtctg gtatcattat ttaacttact actcgatcgg
     1501 gtagcgtcaa aagagatggc acccagcggt gtattccaac catttcccag agttatagca
     1561 ttatagttat cggataaaat cgtaccgcca taaagtgtca aaagattgtt gaggccatat
     1621 atataatttg cctcgagaaa gtcctcctga ttctttacac catatatctt actgcgtccg
     1681 gcgataaaat cgaaatttga aataccaggt tgcagcatgt taggcacgga agagtagggc
     1741 accaaaaagg aacgaacact gccatctgct tcctttatgc tgacatcgag atcagagccg
     1801 ctgcccgaga gttgcaaatc tgcgatagta aatggtcccg ggggaacctc tttttggtaa
     1861 atgatgtagc cattttgcga aaccgttatc aaggcattac tttgcgcaac accttgtact
     1921 aaaggtgtaa agctctgcat agaatcaggt agcatttgca tatcgcgaaa taagcgtatt
     1981 ccgctaaatc tcagagagtc gcatatgagt gaggatgtgt aattttctcc tatctgaact
     2041 gtgcttaata tctgcgatca gccacgttcc aggtaaagag tattactttg ccatgtgcct
     2101 ttcatatcat cgggtttgct atagctggca tcagagtgta actgccaact aaataaattt
     2161 aatccactgt taaatcggcc ataactggct gtattactgt tattattgct gtcataagag
     2221 cgatactgac ttaaattata ggaggtataa aaagcatcaa tgccgcgatc ccaactttcc
     2281 gggggaacgt aacctctttc aagttcattg atatacgcct gtggtactgt taaatttagt
     2341 cggtgttcac tgatatccca ctgatattgt ccaccatgaa ccgctttatc cagtaaaata
     2401 caattatctt ctgtattgag gttaccggtc tttaccccca gtttggttag tagcacctta
     2461 ggcagacatg gtttgactgg tgactctggg atcgtgatat cttgttttcc acgccactgg
     2521 ttatttacat aaaaatcaag ttcgtagttt cctggaagcg gttgtttatt atcgaaatga
     2581 tattcggaaa ctttttctcc tttcatgcca ccgatcataa agtgcgtatc aaaagtttct
     2641 tcagcataac ccggaaaaga atacaaaaaa ataatagcag cgacccatcg tgtcgtcctt
     2701 aacatgactt tttcctgtta tctcgcggcg attttactat ggatgttatt cccgtaatca
     2761 tcaactaccg ttagttcata ttctgatgcg tggttatttt ttaatgtaat gtcgttattc
     2821 gaaaatggag gcagtaagat actttcatta ttaactttca cgttttgtgc tttgatagtt
     2881 gtaaccgtta tccagttggc tgtttcgttg tttattgaga tggcgttgtt tttctttttg
     2941 atatttaatt ga
//
LOCUS       DAZUGK010000324         2943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZUGK010000324 DAZUGK010000000
VERSION     DAZUGK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.326"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1041
                     /locus_tag="ACHY5W_004640"
     CDS             <1..1041
                     /locus_tag="ACHY5W_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449376.1"
                     /translation="HITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTS
                     LEEELNHYFRTGKRKEQQMLEEEDAEQRRSRSGLWGMISQWSGINNPGWEDYRKRDFD
                     PKNPEITLNNRREAIEYIEEIESTVTTLHREAEAQFRPELEKIVRGIETGFRGTALYA
                     TENIAGRINARLEDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETITRRRRKDS
                     FIGKICGWIGTKEWGWETYKEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQ
                     PVRQEIDAFFCTFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITD
                     SKALREELETML"
     gene            1038..1943
                     /locus_tag="ACHY5W_004641"
     CDS             1038..1943
                     /locus_tag="ACHY5W_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFS4449377.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTELTTSLASTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADHVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            1940..>2943
                     /locus_tag="ACHY5W_004642"
     CDS             1940..>2943
                     /locus_tag="ACHY5W_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4449378.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLLQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KRLKHTGDGGNSGGLLSFASAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSEAVL
                     "
BASE COUNT          731 a          709 c          871 g          632 t
ORIGIN      
        1 catatcacca gcctgcttgc ggatatccgg gaaatcgcgg actgtcagga gcaggtgacc
       61 gctgatgtca gaatggccat ggaggagatc gatgccagaa cccgggaatt actcacgggg
      121 gtctgcacct cactggaaga ggagctgaat cactatttca gaaccggtaa acgcaaagaa
      181 cagcaaatgc tggaggaaga agacgcagaa caacgaaggt cccggtccgg attatgggga
      241 atgatttctc aatggtccgg tatcaacaac ccgggctggg aagattacag gaaacgagac
      301 tttgacccga aaaaccccga gataacacta aataatcgca gggaagcaat tgaatatata
      361 gaggaaattg agtcaactgt gaccaccctg caccgtgagg ctgaagcaca gttccggcct
      421 gagctggaga aaatcgtcag gggtattgaa acaggttttc gtggcacggc cctgtatgcc
      481 actgaaaaca ttgccggtcg catcaatgcc cgcctggagg atgagggctt taccgtaaaa
      541 atcagttttc cggcagtcag ccagttacag acccggctcg cagtaaaaac aaatctgagt
      601 gcgcttatgg aggaaagaac ggagacaatc acccgtcgcc gtcggaagga tagcttcatt
      661 ggaaagattt gtggatggat tggcaccaaa gaatggggct gggaaaccta taaggaggat
      721 gtgagccgca gcgtgatcaa tatcaacaag gtcagagagg aagtgatgtc actgacccgg
      781 gcatatttcg gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag
      841 gaaatcgatg cctttttctg tacattcagg gagaaagttg aacagctgcg taacacgctg
      901 attcagagct ctgaagatca taaacgcgat cagcaggcgc aggaacggct taccgggcga
      961 cttcaggcat taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa
     1021 gagctggaga caatgctgtg acttcaccat ttattcaaca aattgctgat aaccgggtat
     1081 gtcaggtact cacctgtctt cctgaaaaat ttgtggttga tttcgcaaac ggtatcgatg
     1141 ttgcacagga gcacatccgc acggccggag agcgcacgtt cttccggcgt ttaaaagagg
     1201 gactgactgg cgaaggcgct gcacggcaga atgccatcaa tgcttcgctt gcacagggcg
     1261 ttgaggcgtc cctgcgctgg ctgacggagc tgacaacgtc gcttgcctcc acaaactacg
     1321 cgattacccg ggtaaacgac agggtcagtt cactggtcag tgatactgcc aggctggcgc
     1381 attattccgc agatacgcgg gagcagttac tcaccctggc cgatcatgtt caccagaaac
     1441 tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc
     1501 ttgagcagat attctcatgg tggagcgccg ggcgatatgc gtcattttcc cctgccggac
     1561 gttgttatgt ggcacttgaa gagcttcgct ggggcgcgtt tggtgatgtg attcgtcagg
     1621 gcgaaacagg ccaggttaac cagctactgg atattctcag acataaagca ttaacgcaga
     1681 tggcacagga gagtggcggt agtgcaaccg tgcgtctgaa cacgctggac tggcttggtg
     1741 gtcagggccg ggagcaggca gataacgagt ggcatgacgc gattaactgg ctgggggact
     1801 ggtgcagtga agagcagcat ccggtgatct ggtcaaccac gcaggctgca gaacacttac
     1861 cggttcgcat gccccgtctc tgctctgcag agcgcctctc tgaaagtatg gtcgatgaaa
     1921 tatttcagaa gggggctgca tgagtacaga aatgaaaacg gggctggtgc tgtccggcgg
     1981 cggggcggtg ggtgcttatc aggcgggagt ggttaaggca ctggcagagt gtggtacaca
     2041 gatcagcatg gtttcagggg ccagcattgg cgcattcaat ggtgccatta tcgcggcctc
     2101 tcccgatctg tcagaagctg ccgtacgcct ggaggcgctc tgggatcatc ttgggaataa
     2161 tcaggtgctg tcggtaaaca gatcggttta cttttcattg ctgaaaaaat tgctccaggc
     2221 aatgaacctc tgccagatcc ccggacgtgc aggagcactg cttacgacgc ttcttcgcca
     2281 tatatcgata atcaacggct tcgacaatcc gatgactcag ccgttgttgt cagatgagcc
     2341 cctgacagcg ctgatggatc attatcttga tactgatgct ctggcagacg ggctaccgct
     2401 gtatgtgtcg ctgtacccca cagaaggggg catgcaggat attattgact gcattcgtgc
     2461 tgaactgggt gtcggaacca cgaaaaacgc cgtttttcag catatccaga gtctgccccg
     2521 cggacagcag aaagaggctc tgcttgcgtc agccgcgctg cccctgttgt tccgtccccg
     2581 tgaggttcag gggacaatgt tcggtgatgg tggtatggga ggatggcgaa atatgcaggg
     2641 aaatacccct gtaacgcctc tggtcgatgc cggatgcaat atggtgattg tgtcgcatct
     2701 gagtgacggt tctttatggg atcgccgggc ttttccggac accacaatcc ttgagatccg
     2761 tccccggaaa aggctgaaac atacaggtga tggtggcaac agcggcggtc tgctcagttt
     2821 cgcgtcggca cataccgacg cctggcgtca gcagggctac gaggacacga tgctgacgat
     2881 ggagcatatc cggaaaccgc tggcagcacg tcaggcactg tcccggtcag aggcggtatt
     2941 gca
//
LOCUS       DAZUGK010000325         2904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZUGK010000325 DAZUGK010000000
VERSION     DAZUGK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.327"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            311..1477
                     /gene="nhaA"
                     /locus_tag="ACHY5W_004643"
     CDS             311..1477
                     /gene="nhaA"
                     /locus_tag="ACHY5W_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFS4449379.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1543..2442
                     /gene="nhaR"
                     /locus_tag="ACHY5W_004644"
     CDS             1543..2442
                     /gene="nhaR"
                     /locus_tag="ACHY5W_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFS4449380.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2480..>2904)
                     /locus_tag="ACHY5W_004645"
     CDS             complement(2480..>2904)
                     /locus_tag="ACHY5W_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFS4449381.1"
                     /translation="TCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFE
                     PSDVEYYFEPVTIQEAGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQ
                     HPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          691 a          642 c          729 g          842 t
ORIGIN      
        1 gcaattattg acgacaagct ggattatttt tgaaatattg gcctaacaaa catcgccgac
       61 tgacaacaaa ttaattatta cttttcctaa ttaatccctc agaaatcctc accttaagct
      121 atgattatca gggcttaggg tcactcgtga acgcttacag ccgtcaaaaa cgcatctcac
      181 cgctgatggc gcaaattctt caatggctcg taaaaaacga attattccta cactataatc
      241 tgattttaac gatgattcgt gcggggtaaa atagtaaaaa cgatctattc acctgaaaga
      301 gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat
      361 tcttatcatt gccgctatcc tggcgatgat gatggccaac agcggcgcaa ccagtggatg
      421 gtatcacgac tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa
      481 aaacatgctg ttatggataa atgacgcgct gatggcggta tttttcctgt tagtcggtct
      541 ggaagttaaa cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc
      601 agttatcgcc gctattggtg ggatgattgt gccggcatta ctctatctgg cctttaacta
      661 tgccgatccg attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcctttgc
      721 acttggtgtg ctggcgctat tgggaagtcg tgttccgtta gcgctgaaga tctttttgat
      781 ggctctggct attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa
      841 tgacttatcg atggcctctc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt
      901 gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctg gttggcgtag tgttgtggac
      961 tgcagtgttg aaatcggggg ttcacgcaac cctggcgggg gtaattgtcg gcttctttat
     1021 tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc
     1081 gtgggtggcg tatctgattt taccactgtt tgcatttgct aatgctggcg tttcactgca
     1141 aggcgtcacg ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct
     1201 gattggcaaa ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc
     1261 gcatctgcct gagggaacga cttatcagca aattatggct gtggggatcc tgtgcggtat
     1321 cggttttact atgtctatct ttattgccag cctggccttt ggtagcgtag atccagaact
     1381 gattaactgg gcgaaactcg gcatcctggt cggttctatc tcttcggcgg taattggata
     1441 cagctggtta cgcgttcgtt tgcgtccatc agtttgacag gacggtttac cggggagcca
     1501 taaacggctc ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac
     1561 aaccacttgt attacttctg gcatgtttat aaagaaggtt ctgtggttgg cgcagcggag
     1621 gcgctttatt taacaccaca aaccattacc ggacagattc gggcgctgga agagcgcctg
     1681 caaggcaaat tatttaagcg caagggacgt ggtctcgagc ccagcgagct gggggagctg
     1741 gtctatcgct atgccgataa aatgttcacc ttgagccagg aaatgctgga tatcgtgaac
     1801 tatcgcaaag aatccaattt attgtttgac gttggcgtgg ctgatgcact ttccaaacgt
     1861 ctggtcagta gcgtgctgaa tgctgcagta gtagaaggcg agccaattca tcttcgctgc
     1921 ttcgaatcca cccacgaaat gctgctggag caattaagcc agcataaact ggatatgatc
     1981 atttccgact gtccgataga ttctacgcag caggaaggcc tgttctccgt gagaattggc
     2041 gaatgtggcg tgagtttctg gtgtacaaat ccaccgccgg aaaaaccgtt cccagcttgt
     2101 ctggaagaac ggcggctttt gattcctggg cgacgttcaa tgttagggcg caaattgctt
     2161 aactggttta actcccaggg attaaacgta gaaatccttg gcgagtttga tgatgccgct
     2221 ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg ttgccccaac gctttatgca
     2281 tatgactttt atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa
     2341 gagtaccatg ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc
     2401 aatacggatt attctgcgct ttttagtcca gcggtgcgtt aatcggcagc tcccccaaag
     2461 ttaaggtggg ggagatagat tagttgtaca ttaccacgat tttgactcgg ctcattattt
     2521 gcccgcttga gacattgttt ccatatgtac gcgggcgaat aaatagagga atctgattac
     2581 ttccttcatg gggatgctga aaagagtagt aattgctttt accatactgg atttcattcg
     2641 taccatcatt ggagagttga aagcctattt gacccgcacc gtcctcactg ttttctaact
     2701 cattttttaa tatgctggtt cctgcctcct gtatcgtcac tggttcaaag taatattcaa
     2761 cgtcactggg ttcaaaaact aattcacctt gctcgccctg gatgcagtca aatcttatat
     2821 gatgaaactg ataattttca ttattgatac gagacagtac aaatcgtcca gtgtcgatgg
     2881 attgatcttc aaaaccacag gtca
//
LOCUS       DAZUGK010000326         2870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZUGK010000326 DAZUGK010000000
VERSION     DAZUGK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.328"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..384
                     /locus_tag="ACHY5W_004646"
     CDS             <1..384
                     /locus_tag="ACHY5W_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS4449382.1"
                     /translation="SSLPQGVALTGDGGNNLLAAFYIQTDVKDLNVMYHNAKVATNNL
                     KLGSFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRGATYPVLIDSIAVEVNSTDTAA"
     gene            521..700
                     /locus_tag="ACHY5W_004647"
     CDS             521..700
                     /locus_tag="ACHY5W_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS4449383.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRVMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            741..986
                     /locus_tag="ACHY5W_004648"
     CDS             741..986
                     /locus_tag="ACHY5W_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS4449384.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            1064..1279
                     /locus_tag="ACHY5W_004649"
     CDS             1064..1279
                     /locus_tag="ACHY5W_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449385.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1284..1817
                     /locus_tag="ACHY5W_004650"
     CDS             1284..1817
                     /locus_tag="ACHY5W_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449386.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2092..2661
                     /locus_tag="ACHY5W_004651"
     CDS             2092..2661
                     /locus_tag="ACHY5W_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449387.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            2661..2810
                     /locus_tag="ACHY5W_004652"
     CDS             2661..2810
                     /locus_tag="ACHY5W_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449388.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
BASE COUNT          747 a          628 c          821 g          674 t
ORIGIN      
        1 tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct gctggcagcg
       61 ttctacatcc agacagatgt aaaagacctg aatgtgatgt accacaatgc gaaagtggcg
      121 acaaacaacc tgaaactggg aagctttggc gcatttgata acgaatggca tacgctggct
      181 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
      241 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      301 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      361 aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      421 ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct ggttttttaa
      481 tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt
      541 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg gataccgggt
      601 catgcgtgac gtctggcgca gacagattga caaaagggcc gctgagaaaa tcagcgccag
      661 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      721 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
      781 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa acagaaactt cgccataacc
      841 tggaagcgcg tcttgatgca gaagtggatg ccattctgga tgaactgctg ggcgcaccgg
      901 cagctccgga gccggaaggc atcgcgggtg aggggagtgc ttcagatagc ggtgacccca
      961 caccggacag cgacatgatg atgtaagcat gcgtcaggga ccatcggtgt gtgccggtgg
     1021 tcttttttat tgttgtgagc ttccggattg cgggaggcgg ggtatgaacc agatggaaaa
     1081 aatcacaaca ggtgtgtcat acaccacgtc agcggtggga acgggctact ggttcctgca
     1141 gttgctggac agggtttccc cgtctcagtg ggcggcaata ggcgtgctgg ggagtctgct
     1201 gtttggtctg ctgacgtacc tgacgaacct gtatttcaaa atcagagagg acaggcgtaa
     1261 ggcggcacgg ggagagtaag ctgatgagca ggaaactccg ctatggttta tcggctgccg
     1321 ttctggcgct gattgccgca ggtgcttctg cgcctgaaat cctcgaccag tttctggatg
     1381 aaaaggaagg caaccacacc acggcatacc gtgatggtgc gggtatctgg accatctgcc
     1441 gaggcgccat catggtggat ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa
     1501 aatgcgaccg ggttaacgct atcgaacggg ataaggcgct ggcatgggtg gagcgtaata
     1561 taaaagttcc actgaccgag ccacaaaaag caggtatcgc gtcattttgc ccctataaca
     1621 ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gcttaatgca ggcgatcgca
     1681 ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtatcc
     1741 gttcaaacaa ctgttacggt caggtatcaa gacgtgacca ggagagtgcg ctggcatgct
     1801 ggggtatcga caggtaagca gaatattttg ctgaaaaatg acgttggcca acgcgggtag
     1861 acaacacgaa atcctgcgaa ctggcaaaat gtaagtgaat aaagtcaaca agattgtttc
     1921 atgaagaggc accgtaatgg tgcctttgtc atttctgcgc ttcgcacaag cgtaaataaa
     1981 ccaaagaacc tttcaggatg agccctggtg gataaccggc agtggtctgg ttaaccctct
     2041 ttgggctggt tattcctgtg cgcagggttc atcactaaaa ggaaataacc gatgaatatg
     2101 atgaccgtgc cgtttcacgg cgattctctt tatgtggtta accataacgg cgaaccatac
     2161 gttcccatga aacctgtcgt tgcggggatg gggctggcct ggcaatcaca gttggctaag
     2221 ttaagacagc gttttgcgtc aactataacg gaaatcgtta tggttgctga ggatgggaaa
     2281 cgacgcaata tggtgtccct gccgcttcga aaacttgcag gttggttaca aaccatcaat
     2341 cccaacaaag taaaacccga aatccgcggc aaggtaatcc agtatcagga agagtgtgac
     2401 gatgttctct atgaatactg gacgaagggt tttgtcgtta atccccgtcg aatgagtgtg
     2461 atggaagaac tcaatcaggc ttgcgctgac atgaaacggg ataaaaacat tgccagtgtg
     2521 tttgctaccg ggctgaatga gtggaaacag gttaaatccg cgcatgtatc aaaaatccgc
     2581 acattgataa acgaagcgaa tctgctgatt gattttgtcc tggctgatac agacaaaggg
     2641 aaaataacaa aggcggattg atggagtggt ggctaatgat atcggataaa ctcataacgc
     2701 tggcgaagat cctctgtgta atcgtcggca tttcattttt agtcattctg gttgccattt
     2761 tcttttccac cgcttggcga gtcctgacgt tatcgggact ggtggggtga aagagagatg
     2821 aaccgtgttc tgtgtgtggt gattattgtc ctgctggtag cctgtggtgc
//
LOCUS       DAZUGK010000327         2856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZUGK010000327 DAZUGK010000000
VERSION     DAZUGK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.329"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            389..577
                     /gene="dicB"
                     /locus_tag="ACHY5W_004653"
     CDS             389..577
                     /gene="dicB"
                     /locus_tag="ACHY5W_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4449389.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            574..765
                     /locus_tag="ACHY5W_004654"
     CDS             574..765
                     /locus_tag="ACHY5W_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS4449390.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            858..2856
                     /locus_tag="ACHY5W_004655"
                     /pseudo
     CDS             858..2856
                     /locus_tag="ACHY5W_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
BASE COUNT          841 a          660 c          707 g          648 t
ORIGIN      
        1 gatttcttcg aacgtgcgct tccggttgtg gcactccgcg aaatggcgcg gcggtaagta
       61 tggcggggtt attccttccc cgttgaggac accgggttgt caggttgacc atacgcttaa
      121 gtgacaaccc cgctgcaacg ccctctgtta tcaattttct ggtgacgttt ggcggtatca
      181 gttttactcc gtggctgctc tgccgccctt tttaaagtga attttgtgat gcggtgaatg
      241 cggctaagcg cacgcggaac agttaaaaca agcggtcttt tacgggcgta acgggaatgc
      301 tctgtatccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca ggcactgcat
      361 cacaaaattc attgttgagg acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc
      421 tgaaggttgt tttgaaattg gtgtcactat cagtaaccct gtatttactg aagatgccat
      481 taacaagaga aaacacgaac gggaactatt aaataaaata tgcattcttt caatgctggc
      541 ccgtttacgt ccgatacaaa aaggatgctg gcaatgaata cagcatttgc acttgttctg
      601 acagtttttc ttgtttccgg agagccagtt gatattgcag tcagtgttca caggacaatg
      661 caggagtgtg tgactgcagc aaccgaacag aaaattcccg gtaactgtta cccggtcgat
      721 aaagttattc accaggataa taacgaaatc ccggcaggtc tttaaaacag ttccgtaata
      781 aacatccgat ttcattctta tatgccagca atggcaggga tttgttcacc cttaaatctg
      841 taatgaggta aaacaaaatg agtaaagtct ttatttgcgc cgccattccg gacgaacagg
      901 caataaagga agaaggtgca gtcgctgtag ccactgccat tgaagccggt gatgaatgtc
      961 gcgcccgcgc aaaatttcac tggcaattcc tggaacatta tccggctgct caggactgcg
     1021 cttataaatt tattgtctgc gaggataaac ttggcatacc ccgccctgcc ctcgattcat
     1081 gggatgctga atatatgcag gaaaaccgct gggatgagga gtctgcttct tttgtcccgg
     1141 ttgagactga atcctatccg atgaacgtca cttttgacaa gctggcccct gaagtacaga
     1201 acgctgtcat ggttaagttc gacacatgtg aaaacatcac cgttgatatg gttattagcg
     1261 cacaggaatt gttgcaggaa gacatggcaa cattcgacgg acatatcgtt gaagcgttga
     1321 tgaaaatgcc agaagttaac accatgtatc cggagcttaa gttgcacgcc attgggtggg
     1381 ttaagcataa atgtattcct ggtgctaaat ggcccgaaat tcaggcagag atgcgcatct
     1441 ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa atacacgtct gttgttgatc
     1501 tcgcccgcgc cagagccaat caacagtaca ctgaaaattc aacaggaaaa atcagcccgg
     1561 tcattgctgc cattcatcgc gaatacaagc agacatggaa aacactggat gacgaactgg
     1621 cctacgctct ctggcctggt gatgtggatg ccggaaacat tgacggcagc atccatcgct
     1681 gggcaaaaaa tgaagttatc gacaacggcc gcgaagactg gaagcgtatc tcggcatcaa
     1741 tgcgcaaaca gcctgatgcc cttcgctacg accgccagac tatttttggc cttgtccgtg
     1801 aacgtccgat cgacattcac aaagatcccg tagcactgaa caaatatatc tgcgaatacc
     1861 tgacgacaaa gggcgtgttt gagaatgaag aaacagacct gggcactgtt gatgttctcc
     1921 agtcatcaga aacacaaact gatgcagtgg aaactgaggt atctgatatc ccaaaaaatg
     1981 aaaccgcgcc ggaagctgaa ccatctgtag agcgtgaggg gccgttctac ttcctcttca
     2041 ccgacaagga tggcgaaaaa tacggtcgcg caaacaaact ttctggtctg gataaggcgc
     2101 tgtctgctgg ggctactgaa atcacgaaag aagaatattt cgcccgcaaa aacggtacat
     2161 actcaggttc acaacaaaat actggttgca tctgacacga ccgcacaacc agggtcagta
     2221 aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag ccaatatcag ccagcctgac
     2281 gccgatgaac tgcttgcagt atcacgtggt gaatttgttg aagggattag cgacccgaat
     2341 gatccgaaat gggttaaggg gatccagacc cgcgattctg tgaaccagaa ccagcaagaa
     2401 acggaacaga acgaccagaa agcggaacaa aacagcccaa atacgcaaca aaacgagcca
     2461 gaaacgaaac aacctgaacc agtagtgcaa caggaaccgg aaaagatctg caccgcctgc
     2521 ggtcagagcg gtggcggcaa ctgccctgat tgtggtgcgg tgatgggtga cgcaacatac
     2581 caggaaatat tcgatggaga gaatcagcct gaagttcagg aaaatgatcc ggaggaaatg
     2641 gaaggcactg cgcatcagca caaggagaac actggcggca atcagcatca tgccagcgat
     2701 agtgaaactg gcgaggcgtc agatccctta attaaggcga acggtcatca taatctcaca
     2761 tccaccagca gagcggggat tcatctgatg atcgaccttg aaaccatggg aaaaaatccc
     2821 gatgccccga ttatctcaat aggtgcaata tttttc
//
LOCUS       DAZUGK010000328         2829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZUGK010000328 DAZUGK010000000
VERSION     DAZUGK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.330"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /locus_tag="ACHY5W_004656"
     CDS             <1..181
                     /locus_tag="ACHY5W_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4449391.1"
                     /translation="KEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQE
                     TLKDTWKSADEWFVH"
     gene            complement(178..264)
                     /locus_tag="ACHY5W_004657"
                     /pseudo
     CDS             complement(178..264)
                     /locus_tag="ACHY5W_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(289..446)
                     /locus_tag="ACHY5W_004658"
                     /pseudo
     CDS             complement(289..446)
                     /locus_tag="ACHY5W_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            712..954
                     /locus_tag="ACHY5W_004659"
     CDS             712..954
                     /locus_tag="ACHY5W_004659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449392.1"
                     /translation="MNDNIPTARNHKQSTCITEKNMPIFLNFTAGSILPENELASLRY
                     IVQQNQNDTVIIKERYKMDIRYIESVNGFRKVRTSS"
     gene            1000..1980
                     /locus_tag="ACHY5W_004660"
     CDS             1000..1980
                     /locus_tag="ACHY5W_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HFS4449393.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSH"
     gene            complement(2486..2755)
                     /locus_tag="ACHY5W_004661"
     CDS             complement(2486..2755)
                     /locus_tag="ACHY5W_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4449394.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(2757..>2829)
                     /locus_tag="ACHY5W_004662"
     CDS             complement(2757..>2829)
                     /locus_tag="ACHY5W_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449395.1"
                     /translation="GDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          794 a          768 c          568 g          699 t
ORIGIN      
        1 aaaggaagaa ttaacccgac gcatcagtaa acgttatgac gatgtagagg tgatcgtaaa
       61 agccaccagc aacgatggcc tttctgttac gcgcaccgcc gataaagatt cagctaaaac
      121 ttttgttcag gagactctga aagatacctg gaagtctgct gacgagtggt ttgttcacta
      181 attaacacgt aaaatcggta acggctggaa atcattcaat actcgcacta tcgaaagttc
      241 accagccaac agcggtaagt tctttcatac gacgtgccgc ggatcctatt attctagtat
      301 gaaatcccta accgatttca aattctccag actgctcaga ttctctttta tcccacgctg
      361 gcgctgataa atctcgtcat tgcaatcgac ctgcgctatc gctgccgcag ttcttccagt
      421 ttctgcatcg ataactccat cttctgagca ataaacctca cctatgcaat atcttatgtc
      481 aattatttca attgcattaa tatcttgaat ggattacata gagttaatgt atccagtact
      541 ccctattctc ttgcatataa tacatgttgc aacttacatc tcagcgctat gaaaaaacac
      601 cacctctctc tttatgaaat tctggattta ccaagcgcta atttatcatt tcaatctacc
      661 tttaagcatt gcatttatct ccctacgagg tcatacttta ggaagttaaa tatgaatgat
      721 aatataccta cagcgcgaaa tcacaaacaa tcgacttgta ttacagaaaa aaacatgcct
      781 atctttttaa acttcacagc aggcagtatc ttacctgaga atgagctagc atctttacgt
      841 tatattgtgc agcaaaatca aaacgatact gtaatcataa aagaacgtta taaaatggat
      901 atccgttata tcgaatcagt caatggtttt aggaaggtgc gaacaagttc ctgatatgag
      961 atcatcatat tcatccggag cgcatcccag agggacatca tgagccatca actcaccttc
     1021 gccgatagtg aattcagcac taagcgccgt cagacccgaa aagagatttt cctctcccgc
     1081 atggagcaga ttctgccatg gcagaatatg accgctgtca tcgagccgtt ttatcccaag
     1141 gcgggcaatg gccgacggcc ctatccgctg gagaccatgc tgcgtattca ctgcatgcag
     1201 cattggtaca acctgagcga cggtgccatg gaagatgccc tgtacgaaat cgcctccatg
     1261 cgcctgtttg cccgattatc cctggatagc gccctgccgg atcgcaccac catcatgaat
     1321 ttccgccacc tgctcgagca gcatcaactg gcccgtcaat tgttcaagac catcaatcgc
     1381 tggctggccg aagcaggcgt catgatgacc caaggcactt tggtggatgc caccatcatt
     1441 gaggcaccca gctctaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc
     1501 aagaaaggca atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt
     1561 ggcctgaccc acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt
     1621 aatctgcttc atggagagga gcaatttgtc tcagccgatg ccggctacca aggagcgcca
     1681 cagcgcgagg agctggccga ggtggatgtg gactggctga tcgccgagcg tcccggcaag
     1741 gtaaaaacct tgaagcagca tccgcgcaag aacaaaacgg ccatcaacat cgaatacatg
     1801 aaagccagca tccgtgccaa ggtagagcac ccgtttcgca tcatcaagcg gcagttcggc
     1861 ttcgtgaaag ccagatacaa ggggctgctg aaaaacgata accaactggc gatgttattc
     1921 accctggcca acctgtttcg ggtggaccaa atgatacgtc agtgggagag atctcactaa
     1981 aaactgggga taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat
     2041 ttacgggaga aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag
     2101 aatttccccg cttattcgca ccttccttaa tatcctgttt ctgagcacat taactccatc
     2161 attaaaagca atattcaata attctgcata aacccgtccc cgcccttcac tttctgctaa
     2221 aaaacggcct gtatctggca tataagaaac tctaaccctc tcccctccca ggagaaatcc
     2281 accaatctgc ccctccatta cctgctgact gacgcagctc catcatctca ttcacgaatg
     2341 aagccgatga cacatatgat attaatccag gcattaccat attccttcat acggataaat
     2401 attccctcaa gtctttatta acaaacatca ccatgacatg acaacaaaaa ccggagccgg
     2461 actccggttt ttgtgaagct gtcggctatt tcattccgcc aatattttcc cacctcccgt
     2521 cagcacgcag gatttgcagc ggtcttacca cgcactgtat ctgcttttta tccgcatcca
     2581 gtatcaccac ctgcgtgatt accctgtcct gctccgggat aatgccattc tcatctgact
     2641 ccaggatgtc tgccggcccc agtcgcagct gtgctgtaag cgactgcccg tgttcacagt
     2701 catcatgctt tccgcaacca cacagacgct ccataagctt tctcaaaata ttcatgtcat
     2761 tctcctgttc tgcctgtatc actgcccact tcatccagtc ccttaacatc ctgccacggc
     2821 ccgtcgcca
//
LOCUS       DAZUGK010000329         2759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZUGK010000329 DAZUGK010000000
VERSION     DAZUGK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.331"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..231
                     /locus_tag="ACHY5W_004663"
     CDS             13..231
                     /locus_tag="ACHY5W_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFS4449396.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            233..790
                     /locus_tag="ACHY5W_004664"
     CDS             233..790
                     /locus_tag="ACHY5W_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4449397.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1081..1236
                     /locus_tag="ACHY5W_004665"
     CDS             1081..1236
                     /locus_tag="ACHY5W_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS4449398.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1356..1700
                     /locus_tag="ACHY5W_004666"
     CDS             1356..1700
                     /locus_tag="ACHY5W_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFS4449399.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1822..2094
                     /locus_tag="ACHY5W_004667"
     CDS             1822..2094
                     /locus_tag="ACHY5W_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449400.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2096..>2759
                     /locus_tag="ACHY5W_004668"
     CDS             2096..>2759
                     /locus_tag="ACHY5W_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS4449401.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKN"
BASE COUNT          728 a          631 c          746 g          654 t
ORIGIN      
        1 gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca
       61 gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg
      121 gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg
      181 gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac
      241 tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc
      301 gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc
      361 actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca
      421 tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct
      481 tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc
      541 ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat
      601 gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt
      661 aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc
      721 gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt
      781 gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg
      841 gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt
      901 agaattgcag cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag
     1201 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc
     1261 ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg
     1321 ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac
     1381 ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag
     1441 cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg
     1501 aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga
     1561 caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca
     1621 aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg
     1681 cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc
     1741 tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac
     1801 ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac
     1861 ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg
     1921 ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt
     1981 gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt
     2041 cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg
     2101 ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg
     2161 tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat
     2221 gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc
     2281 actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact
     2341 gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca
     2401 cagcgaaacc gttattcaca gttacggtgc tggcgcaatg gtgttgtgct ggcactgcga
     2461 caaccagctg cgcgaccaga cctccgaatc acttgagcaa cttactcaac aaaatctgac
     2521 agcctggatg attgacgtca tacgccatgt aatgaatggc acgcaggagc gggaattatc
     2581 gctggctgaa ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc
     2641 agtatcgcgt cgctctctgg gattaccggc ggaaaaaatc cgctccgtat accgtgaaag
     2701 cgacatcata ccgggagaac agaccgccac cagcatactg aagcagcgca caaaaaata
//
LOCUS       DAZUGK010000330         2757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZUGK010000330 DAZUGK010000000
VERSION     DAZUGK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.332"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..158)
                     /locus_tag="ACHY5W_004669"
     CDS             complement(<1..158)
                     /locus_tag="ACHY5W_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFS4449402.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYL"
     gene            complement(294..749)
                     /locus_tag="ACHY5W_004670"
     CDS             complement(294..749)
                     /locus_tag="ACHY5W_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449403.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
     gene            complement(742..1029)
                     /locus_tag="ACHY5W_004671"
     CDS             complement(742..1029)
                     /locus_tag="ACHY5W_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFS4449404.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            complement(1022..1612)
                     /locus_tag="ACHY5W_004672"
     CDS             complement(1022..1612)
                     /locus_tag="ACHY5W_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS4449405.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSLSVAAKSAAGRENPCSSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GILTPVWAIAIERENSGDSSYLLLEGGCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            complement(1609..1875)
                     /locus_tag="ACHY5W_004673"
     CDS             complement(1609..1875)
                     /locus_tag="ACHY5W_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449406.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            2427..>2757
                     /locus_tag="ACHY5W_004674"
     CDS             2427..>2757
                     /locus_tag="ACHY5W_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFS4449407.1"
                     /translation="MSDNTIPEYLQPALAQLEKARVAHLENARLMDETVTAIERAEQE
                     KNALTQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACD"
BASE COUNT          684 a          697 c          777 g          599 t
ORIGIN      
        1 agataaaagc cttccagctc agcggcgatg gccgcctcca gtgactcaag ggtgagatgc
       61 gggtagcggt gctgacgttc gcacacggtc agccaggcac aggcgacagc gcgacggtaa
      121 agggcagcgc gtaagacggg cggtaagggt gttttcattt gcttttctcc ctgtgacaga
      181 tgactgcatt ccgtgccggt tgcattaact gataaggtat atctgcgtct cctgaagacg
      241 tgcgtatccc tgcgcgaata cgcacattta atttttcggg ggtcgttttt taattacaga
      301 taattgcggt aactgttatc cggggtggtt tccgggtcag gctccgtgcg gggaatttcc
      361 cgccattccc gcgccaccgg tgctgcccgg ctgaccggaa cagtgtcctg cgggtaaata
      421 tccagatatt tttcccgcca tttctgtaat tccgggtctc cggccatttc tttcagtacc
      481 gcatgccggt ttacggggct gcgtttaaac aggtcaggac ggtcacaggt aaattcccgc
      541 agaaaacgcc ccagcgggat gtctgtggtg cgtccgtcag cgaggatacg cacaaggata
      601 ctgaatttac ggcggtacgg gttccagaca atttccgggc agcggtacgg catttcccac
      661 ggaataccgt cttccagaat gccgaccacg gccacatcgg gaaaaccggc agaacggtaa
      721 atctcaccgg gctggggaaa atcaaacatg cgtcctgtct ccccggtctt tctgctgggc
      781 gagaaaatcg cggcacaggc ctttggcttt cagttcattc agcacaaaat caatatcttc
      841 attcaggtag ctgaaaatat gcggaatgta gagctgatgc aggccggaga gttcacggtg
      901 aatcaaatca cccccaacaa actgggatac ggcgctggcg cggttgagct tatggtaagc
      961 ctcaatgctg aggtgttcac gggcgtcatg acgcgctgag acggtctgag gggctttttt
     1021 attacgcacg ggacacctcc accaccggca gacgggcagc aagggagagc acatagtcac
     1081 ggacaaggga acggcgggca ctgcgttcat caccggcgac ggtgcgaagc atacagatac
     1141 ggggatgacg gtctgcgcga cggacagccg caaacacaaa gacaaattca gggtgtgagg
     1201 gggtaagggt tgtagccata aggcagcctc cttcgagtag caaataactg ctatcgccgg
     1261 agttctcacg ctcgatggcg atagcccaga cgggggtgag aataccggcc tcgaagaata
     1321 ccggccagcc cggaggctgc cccgcctgag ctaccattga ctcagtggca taacatgcga
     1381 ttgcgaacag gatcatacct gcacggcaaa ccacacgcca caccataatc tggcgctctg
     1441 tggcgttgat tgcgacacaa aaaaagacgc atggcgcgtc atatgtcgcc ttcgagctac
     1501 acgggttctc acgcccggct gccgattttg cggcaacgga aagactatat ccgcaaatgc
     1561 cggaaaaagg caagccagaa aaagggagtt tttgcagagc gggcatcatc atgcgtcata
     1621 cccccgtttg cgtccggcaa tgcggccggc catccatgcg gtgacttcag agtgcagcca
     1681 ggccacattt ttaccgccaa gactcacctg cggcggaaat tcccccttac ggatgagttc
     1741 atagatggtc gagcgtgaca ggccgcacag gtgcatcact tccggcagac gtaaaaaacg
     1801 ctcctgcgtg atgtccggca gcggcatcag tggcgtcaca ggggcgggag acggggaaga
     1861 aaaaacagct tgcatcgggc tacctcgtta atgtccatac agcaccggat aagtccgtcc
     1921 ggcttcgggt agcgctttat tttgtgaata tttttggcag acgcaacagg ggggatttgt
     1981 tccggcagcc ttacaatggc tgtgcgtttt ttgtacatca gcgccagata gctttaaaac
     2041 gctctggaag gagctggaaa aaattatagt gaaatacaaa ttgttttttc ttatttattt
     2101 cagtgaatta ataaaaataa aaagtaataa acagcacaaa aagcccatca acgggtgaac
     2161 agtggtgaac agacggtgaa cagtcattac tgcgattgtt caccatttaa cttactgtat
     2221 tacttatctt ttttcttatg gtgaacagag gtgaacagta aaatataaaa aaacaaacag
     2281 taagccggtt tttcctgcga ccttttcctg gcttgccggt ctgaggatga gtctcctgtg
     2341 tcagggctgg cacatctgca atgcgtcgtg ttgttgtccg gtgtacgtca caattttctt
     2401 aacctgaagt gacgaggagc cggaaaatgt ctgacaacac catccctgaa tatctgcaac
     2461 ccgcactggc acaactggaa aaggccagag tcgctcatct tgagaacgcc cgcctgatgg
     2521 atgagaccgt cacggccatt gaacgggcag agcaggaaaa aaatgcgctg acgcaggccg
     2581 acggaaacga tgctgacgac tggcgcacgg cctttcgtgc agccggtggt gtcctgagcg
     2641 acgagctgaa acagcgccac attgagcgcg tggcacgccg ggagctggta caggaatatg
     2701 acaatctggc cgtggtgctg aattttgaac gtgaacgcct gaaaggggcg tgtgaca
//
LOCUS       DAZUGK010000331         2748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZUGK010000331 DAZUGK010000000
VERSION     DAZUGK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.333"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(111..>2748)
                     /gene="cas3"
                     /locus_tag="ACHY5W_004675"
     CDS             complement(111..>2748)
                     /gene="cas3"
                     /locus_tag="ACHY5W_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFS4449408.1"
                     /translation="WGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQSVVLQNTFCR
                     NEMLSKQRVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLNGPSTQMCRKF
                     NHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHHGFILHSQDQD
                     KSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCSSLLAGFCSLA
                     DWLGSWTTTDTFLFKEDAPSGIQAVRTYFQDRQQDACRVLALSGLVSNKRRYDGVHAL
                     LDNGYQPRQLQVLVDALPTAPGLTVIEAPTGSGKTETALAYAWKLIDQQLAESVIFAL
                     PTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFTEQGQEEAWVQ
                     CCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLIVDEVHAYDTY
                     MNGLLEAVLKAQADVGGSVILLSATLPMKQKQKVLDTYGLHTDPVENNSAYPLINWRG
                     VNGAQRFDLLAHPEQLPPRFSIQPEPIYLADMLPDLTMLERMIAAANAGAQVCLICNL
                     VDVAQLCFQRLKELNDTQVDIDLFHARFTLNDRREKENRVISNFGKNGERNVGRILVA
                     TQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEIPLATVLLPDGEG
                     YGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEMDEPEWVIKGM
                     DKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLPYVQTSSGKQL
                     LDGQVYEDLSHEQQYEALALNRVNVPFTWKRSFSEVVDEDGLLWLEGKQNQDGWFWQG
                     NSIVITYTGDEGMTRVIPANPK"
BASE COUNT          772 a          658 c          565 g          753 t
ORIGIN      
        1 tctcataccg ggaaattaaa agaagatgta cattgtgcac cttccctact taagtaggga
       61 taaaccgtta ttggtcttgt tatcgtcatt gataccaatc attcccgaag ttatttggga
      121 tttgcaggga tgactctggt catcccttca tcccctgtat aggtaataac aatactgtta
      181 ccctgccaga accatccatc ctgattctgt ttcccttcca gccaaagtaa cccatcttca
      241 tctactactt cagaaaaact acgtttccag gtgaagggta cattgacgcg attaagtgca
      301 agcgcctcat actgctgttc atgacttagg tcctcgtaga cctggccatc gagcagttgt
      361 ttacctgaag acgtttgtac ataaggcaat aatggcaggc tcatttcccc atccctcgtt
      421 accgcaagaa tggtttcatc gttatcctgc aaactatatt cttcagccca ctgcagaacc
      481 ttgcgagcct tgaacctctt ttcacactca gcgctttcaa acttatccat gcctttgatg
      541 acccattctg gctcatccat ttcagcatca tcgtaaatgc tatccagcca ttgccggtag
      601 gcatcaggga aaaataagga tgctccatta agttcctcaa tatgttgctg cgtccgccac
      661 atgactctaa cgttgctata aatatgctca tgtcgtccgt aaccttcgcc atcaggcagc
      721 aaaacggttg ccagaggaat ctcaaaaccg gcgggacgat atttacgatg atggcgatgt
      781 aaacggccta atcgttggaa aagcaaatca gcgggacaat gctgggtaat taaccaatca
      841 aaatctacat cgagtgattg ttccacgacc tgggtggcga caagtattcg tccaacattt
      901 cgctccccat tttttccgaa attgctaatg acacgattct ctttttcacg acgatcgttc
      961 agcgtaaagc gcgcatgaaa caaatctata tctacttgcg tgtcatttag ttcctttagc
     1021 cgttggaagc atagttgtgc aacgtcaacc aaattgcaaa tgaggcagac ttgtgcaccc
     1081 gcgtttgccg ctgcgatcat tcgctctaac atcgtaaggt caggtaacat gtcagctaaa
     1141 taaataggtt ctggctgaat cgaaaagcgg ggcgggagtt gttctggatg agctagcaga
     1201 tcaaaacgtt gcgcaccatt cacacctcgc cagttaataa gtggatatgc ggagttattt
     1261 tccactggat ctgtatgcag accataagta tccagaactt tctgtttttg tttcattggt
     1321 agggttgcgg aaagaagaat aacactccct cccacatcag cctgagcctt gagcactgcc
     1381 tccagcaagc cgttcatata ggtgtcgtaa gcatgaactt catcaacaat taaaacactt
     1441 cgaccaattc ccaaaccacg gataaagcgg tgtttaactg gcaataccga tatcaacacc
     1501 tgatcaatag tgcaaacgcc gatttgccca agaaacactt tcttattgct ttgtgacaac
     1561 cactgacaac actgaaccca cgcttcttct tgcccctgct cagtaaatgc gcgtgatttt
     1621 attgattgaa aaaggtggtt aaaacgtgaa ttaccatgag caagaataag atttggggag
     1681 gtaaataaac ggctcgcgtt cgcttccatt ctgctgagca ttgcatttgc agtagcttgt
     1741 gtagggaggg caaaaataac actctccgca agttgttggt caataagttt ccaggcatag
     1801 gccagcgctg tttcggtttt acctgatccc gtaggtgcct caatcaccgt cagcccggga
     1861 gctgtaggaa gagcatcgac caacacctgt aattgtcttg gttgatagcc attgtccagt
     1921 aaggcatgta cgccgtcata acgacgctta tttgatacaa ggccactcag cgccagtacc
     1981 cgacacgcat cctgctgtct atcctgaaaa tatgttctta ccgcttgtat tcctgaaggt
     2041 gcatcttctt tgaatagaaa ggtatccgtt gtggtccagg aacctaacca gtcagcaagc
     2101 gagcaaaaac ctgctaacag tgatgaacaa tcaggtggta tatcgtttat agataacccg
     2161 gctggcgtta aaaataatgc ttcgagtaca gatatccact cctcacgagc ctgtttatct
     2221 tgcgcagcat aagatgccaa agaagctggc atttcccaac gcgacttatc ttgatcctgg
     2281 gaatgtaata taaaaccatg atgtcctgta acggcctcta cccatggaaa ccaggactca
     2341 taaggatgag gagctgcatc aaaaaaactg aaaaaatccc cgggagattg ctctgaaagt
     2401 gaatcttggt taaaccaata cagaccggct gcaccatgat taaatttacg gcacatttgt
     2461 gttgatggac cattaagtga tggcgttgca ggatttaatt tcagccaact ttctgctgat
     2521 ttatattgga atcgtatatc aaactttcca atatcatgaa gagcaatgaa aaataacagc
     2581 caggccttca ccctctgttt tgatagcatt tcatttcggc aaaaagtatt ttgcagtacg
     2641 actgattgat cccaccagca atctgcaaca gcagcaacat caaggcaatg ataaattaac
     2701 agatgaatat catttccttt cgtcaggctt tttgaggatt ttccccag
//
LOCUS       DAZUGK010000332         2741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZUGK010000332 DAZUGK010000000
VERSION     DAZUGK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.334"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..2356
                     /gene="ydeP"
                     /locus_tag="ACHY5W_004676"
     CDS             77..2356
                     /gene="ydeP"
                     /locus_tag="ACHY5W_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFS4449409.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2604..>2741
                     /gene="safA"
                     /locus_tag="ACHY5W_004677"
     CDS             2604..>2741
                     /gene="safA"
                     /locus_tag="ACHY5W_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFS4449410.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     IS"
BASE COUNT          729 a          646 c          671 g          695 t
ORIGIN      
        1 cgataatccc tttgtatgta ctgtgcatca tcgctattac aaatcctaat aattcatttc
       61 cacacaggat aagtagatga agaaaaaaat tgaatcctac cagggtgctg ccggtggttg
      121 gggtgctgtt aaatccgtag cgaatgcagt acgtaagcag atggatatac gccaggatgt
      181 tattgccatg tttgacatga ataagccaga gggctttgac tgtccgggtt gtgcatggcc
      241 agatcctaag cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag caatcgcctg
      301 ggaagtcacg gataagcagg taaacgcctc tttctttgct gagaatacgg ttcaatcatt
      361 acttacctgg ggagaccacg agcttgaggc tgcggggcga ctcactcagc ctttgaaata
      421 tgatgccgtc agcgactgtt acaagccatt aagctggcaa caagctttcg atgaaattgg
      481 cgcacgcctt caaagctata gtgatcccaa tcaggttgaa ttctatactt cgggccgcac
      541 ttccaatgaa gctgcctttc tttatcagct ttttgcccgt gaatacggga gcaataactt
      601 tcccgactgc tccaacatgt gccatgaacc gacaagcgtg ggtttggcag cgagtatcgg
      661 tgtaggtaaa gggaccgtgt tgctggaaga ctttgagaag tgcgatttag tcatttgcat
      721 tgggcataac cctggtacaa accaccctcg catgctgact tcgttgcgcg ctttagtgaa
      781 acggggagcg aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg agcgatttac
      841 cgcaccgcaa aacccgtttg aaatgctgac gaactctgag actcagttgg ccagtgccta
      901 ctacaacgtg cgcattggtg gcgatatggc gttgctcaag gggatgatgc gcctgttaat
      961 tgagcgcgat gatgctgcaa gtgccgcagg tcggccctca ttgcttgatg acgaatttat
     1021 tcaaacgcat accgtcggct ttgacgagct acgccgtgac gttctcaatt ccgagtggaa
     1081 agatatcgaa cgtatttctg gactaagtca gacacaaatc gccgaactgg ctgacgcata
     1141 tgccgctgcc gaacgcacca ttatctgtta cggaatgggg atcactcagc acgaacatgg
     1201 tacccagaat gtacagcaac tggtcaatct gctgttgatg aaaggtaaca ttggcaagcc
     1261 tggagcgggt atctgcccac tacgtggcca ctctaatgta cagggcgacc gaaccgtcgg
     1321 tatcaccgag aaaccgtctg cagagtttct ggctcgcctg ggtgagcgct atggcttcac
     1381 cccacctcaa gcacctggac atgctgcaat tgccagcatg caagcaatat gtacagggca
     1441 ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag atcgggaagc
     1501 gagcgctgta ccgttaacgc aattagatct ggcggtacac gtagccacta agcttaaccg
     1561 ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctgggac gtagcgagat
     1621 tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt cgatgattca
     1681 tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt gtgcagtggt
     1741 cgcgggaatc gcgcaggcag cactacccca gagcgtggta gcctgggagt atctggtgga
     1801 agattatgat cgcattcgca atgacattga agctgtgctg ccagagttcg ccgattataa
     1861 ccagcgcatc cgacatcctg gtggttttca cctgataaac gctgctgctg aaaggcgctg
     1921 gatgacgccg tcaggtaagg ctaatttcat taccagcaaa gggctgttag aagatccctc
     1981 ttcagcgttt aacagtaagc tggtcatggc gacagtacgc agtcacgatc agtacaacac
     2041 gacgatttat ggtatggatg atcgttatcg aggggtattc ggtcaacgag atgtggtctt
     2101 tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag ttaatcttat
     2161 tgcgcttacg ccagacggta agcgcagctc acgccgcatg gatagattaa aagtggtcat
     2221 ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc acatgctaac
     2281 acttgataac cacgatccat taagtggcat tcctggctat aaaagtattc cggttgaatt
     2341 agaaccatca aattaatgcc tcttctcatt tcttctgctg tcatccgcac agcagaagaa
     2401 ttcctcattg actattatcg cgcaatttgc tcacatggat taaattaaac tacatactat
     2461 aagatataaa cttctgccta cagctgtaag aaactccgct cagtactgaa gcaccagtcc
     2521 tatttcctct tttctccagc ctgttatatt aagcatactg attaacgatt tttaacgtta
     2581 tccgctaaat aaacatattt gaaatgcatg cgaccacagt gaaaaacaaa atcacgcaaa
     2641 gagacaacta taaagaaatc atgtctgtaa ttgtggttgt cttattactg acccttacgt
     2701 tgatagccat tttttcggca attgatcagc tgggcatttc a
//
LOCUS       DAZUGK010000333         2711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZUGK010000333 DAZUGK010000000
VERSION     DAZUGK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.335"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..1312
                     /gene="dadA"
                     /locus_tag="ACHY5W_004678"
     CDS             14..1312
                     /gene="dadA"
                     /locus_tag="ACHY5W_004678"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFS4449411.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            1322..2392
                     /gene="dadX"
                     /locus_tag="ACHY5W_004679"
     CDS             1322..2392
                     /gene="dadX"
                     /locus_tag="ACHY5W_004679"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFS4449412.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT          591 a          687 c          821 g          612 t
ORIGIN      
        1 tatcggagtg gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc
       61 ctggtactta aatcaggcag gacatgaggt caccgtcatt gatcgggagc cgggggccgc
      121 cctggagacc agtgccgcga atgccgggca aatctccccc ggatatgctg caccgtgggc
      181 ggcaccaggt gtgcctttaa aagcgatcaa atggatgttc cagcgccatg cgccgctggc
      241 agttcgtctc gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg
      301 cgacaccagc cactacatgg aaaacaaagg gcggatggtg cgtctggcgg aatacagccg
      361 tgattgcctg aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg
      421 gacgctgcaa ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt
      481 gctggaagat gccggcgtac cgtatcagct gctggaatcc agccgcctgg cggaagtgga
      541 gcccgcgctg gcagaagtgg cgcacaaact gacgggcggc ctgcagttac ccaatgatga
      601 aaccggagac tgtcagctat ttacccagaa tctggcgcgg atggcggagc aggcgggggt
      661 taaattccgc tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatctacgg
      721 cgtgaagtgt ggcgatgaag tgattaaggc cgatgcgtat gtgatggcgt ttggttctta
      781 ctcgacggcg atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc
      841 gctgactatt ccaattgcgc aggaagatgg tgcgccagta tccaccattc ttgatgaaac
      901 ctacaaaatc gccattaccc gtttcgataa ccgcattcgt gttggcggaa tggcggaaat
      961 tgttggtttt aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg
     1021 cgatctctat ccacgcggcg gtcatgtcga gcaggcgact ttctggacgg gtctgcgccc
     1081 gatgacgcca gacggtacgc cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa
     1141 taccggtcac ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga
     1201 cctgctctct ggtcgcacac cagcgatccc atatgaggat ctaagcgtag cgcgctacag
     1261 ccgtggattt acgccatcac gtccgggcca tttacatggc gcacacagct aaggaaacga
     1321 gatgacccgt ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat
     1381 tgtccgccag gccgcgccgc acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg
     1441 gcatggtatt gagcgtatct ggagcgcgct cggggccacc gatggctttg cattgcttaa
     1501 cctggaagag gcaataacgt tacgtgagcg cggctggaaa ggaccgatcc tgatgctgga
     1561 aggatttttc catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt
     1621 acacagcaac tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat
     1681 ttatcttaaa gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac
     1741 cgtctggcag cagttgcggg caatggcgaa tgttggcgaa atgaccctga tgtcgcattt
     1801 tgccgaggcg gaacatcctg atggaatttc cggcgcgatg gcgcgtattg agcaggcggc
     1861 ggaagggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcacccgga
     1921 agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc cgtccggtca
     1981 gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca gtgagattat
     2041 tggtgtccag acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg
     2101 cgatgaacag cgaatcggca ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc
     2161 gcctaccggc gcccctgttt tagttgacgg cgtgcgcacc atgacagtag ggacagtttc
     2221 gatggatatg ctggcggtcg atttaacgcc ttgcccgcag gcgggtattg gtacgccggt
     2281 tgagctgtgg ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg
     2341 ctatgagttg atgtgcgcgc tggcgttacg tgtcccggtt gtgacggtgt aacttgttgt
     2401 aagccggatc ggaggcaacg tcttctggtt gcaaaaaaaa tcatccatcc ggctggtcag
     2461 caactgtagt tgttaatgtg acagagccat tgcccatgat agtgcccatt aaaatgatgg
     2521 acactatttc cccggaacct gaactcaccg cacaggcgtt ctacataaaa cgcttacgtt
     2581 tcattgttga ctcgaactcg acttcagata aatcacgctt cacccttgat gcaggttcgg
     2641 gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca
     2701 ggcccaactg c
//
LOCUS       DAZUGK010000334         2692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZUGK010000334 DAZUGK010000000
VERSION     DAZUGK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.336"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHY5W_004680"
     CDS             complement(<1..67)
                     /locus_tag="ACHY5W_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449413.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNG"
     gene            complement(75..470)
                     /locus_tag="ACHY5W_004681"
     CDS             complement(75..470)
                     /locus_tag="ACHY5W_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS4449414.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(467..1045)
                     /locus_tag="ACHY5W_004682"
     CDS             complement(467..1045)
                     /locus_tag="ACHY5W_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449415.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1057..1410)
                     /locus_tag="ACHY5W_004683"
     CDS             complement(1057..1410)
                     /locus_tag="ACHY5W_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4449416.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1422..1817)
                     /locus_tag="ACHY5W_004684"
     CDS             complement(1422..1817)
                     /locus_tag="ACHY5W_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4449417.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1859..>2692)
                     /locus_tag="ACHY5W_004685"
     CDS             complement(1859..>2692)
                     /locus_tag="ACHY5W_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4449418.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
BASE COUNT          561 a          840 c          678 g          613 t
ORIGIN      
        1 tgccgttgta aacccacaga gtggtaccgg caccttttac cggctcaagg ggatttggtg
       61 ttgccatatc gtcctcacat ctcgtatgta atggaataag tcagatctgc agaactccat
      121 aacgccatat cgtcatcacg acgatactca tagccctgcg taaccatcgt ggtaatcagt
      181 cctgccagtg ccgggatatc gctcatcacc ggataaatcc gggactccat ccacgcatcc
      241 agctctgaat ccggcacctg agcaggcagg aaaacttcaa tatgcagctc cgcctgccag
      301 gtatcgctgt ccagctcttc gcccgtgtat tcagcgccgg tgagataaac ggcaactgcc
      361 ggaaaatccg cctcatcaaa aacagcgggg cgaccatcaa aaagcgtcgc cccggtgtca
      421 tgcttctcca gtgcatccag tacggctgca cggagttcag tatgtttcat cgctttatta
      481 ccattctcag ttgatgctgc agcgcatagc ccagctcttt cggaagacgt tcacgccgta
      541 tccgttcaat attctgttta aacgccgtgg taagcggcac cgccatcggg attttcacca
      601 catcaatggg gtaacggttt ttcccggcca cacgctgcat gacatgccac cgcccatttt
      661 tcagttgctg aataaacgcg ccgggaatac gacggttacc caccacaagc acgctgccgc
      721 cacctttcag ggatgaacgc tgcccctttt tacgacgcct gcgtcgggac aggacaaccc
      781 gcgcattccc cagcttgatt acgggcaaat ccccccggtt aaccctgatt ctggcctgcg
      841 gatttttaac cgtggccctt ttcagcctgg ccctttcctt taccagtttc cggcgtacct
      901 ttgtctcacg ggcaacctgt gacgcagact gcgatatcgc ggatgaagca acgcggttaa
      961 tggccattgc tgacgcaccg ggcaccgccg ttttgctgat acggctgagg ttttcaacgg
     1021 cctgctcaag accttttatg gccatacatc cccctttcag cggcgacggt taacggcagg
     1081 cggcacgcca cgcccaagcc agagatgaca gcttccgcca tcatccggcg aaatccggtc
     1141 tatccagaag ttttcctcac cgatggtcag cgtgtctcca cgccgcagtt gccgcacatc
     1201 atcagtccgg acaaacaggg acgggctgga gccttcaacg cgcacgccct gtccggcata
     1261 gctgatattt tcagggtcat caaaaacacc acgtattact gcgccggact gctcaccgga
     1321 tgtcatggtg gctgacgttc ccatgtaccc gcgtatcgtt tcatcggcgc gggcaatggc
     1381 agcatcgaac aggttatcga aatcagccac agcgcctccc gttattgcat tctggccagg
     1441 ccacgttctg tcatttcggc tgccacaccg gcagagacac ggaacgccgt tcccggcagc
     1501 acaaatgcca caggttcatc ccgcgtggcg tgaagtgcat cggtatgcag cgtcaccagt
     1561 gccacaaccg tgaccagagc agccgtatca gtcacggtat ccgtctgtgc tgataccacc
     1621 tcattttcat gtccggtcag cgcattttcc gggctgacag acgtgtcctg accggctgcg
     1681 tcatccgtgt catcaagctc ctcttccagc tctgccacac ggagcgccag ttcttctttc
     1741 gtccccgtca ggctgacatc acggttcagt tgttcaccca gcgagcggag acgggcaatc
     1801 agttcatctt tcgtcatgga ctcatccaca gagaaacaat ggccccgaag ggccataatt
     1861 acgccagttg tacggacacg aactcatcag ggtcagccag cagcatcagc ggtgctgact
     1921 gaatcatggt gaactctcgc gccggatcgc cggtagtcac ccagtttttc gggtaacggg
     1981 cagaggcgtt aatgccttcg cgctgtgcgt ccgcatcctg aatacagcca taggtacgca
     2041 gaccgcgtgc ctgagtgttc cccagcacca tcgtgttgtc cggcaggaag ttctttttga
     2101 cgccgttttc cacgtactgt ccggaataca cgacgatggc cacatcgcca tacattccct
     2161 tataagacac cgctttgccc aggtctttta ccgctgtctc cagctcggaa tgagagccgc
     2221 gacgggtatc cagcttatcc ctgacggctt tgaaggaacg gaacagcgcc catcctttcg
     2281 gatcaaacac gatgatattc accacgccgc tggcgttcag cgcgtaggct tcgatatcgt
     2341 cggtcgggtc atacgtggac ttgtcacgct tgctccactc cgtgccaccg gactgtgtga
     2401 tgttgtttgc cgcactgcgg cccatatcca cctcaaccgg atcaaaggct tcaccggtca
     2461 tggtgtattt gcccttaagc acggcagaaa ctgcctgcat ctcttcgacc tgggcaatgg
     2521 ccagctcttc gtcacgcatg ttctgcatga tgatgcgacg gcggcggtaa gccgggtccg
     2581 ccagattctg cggatcttca tccggcaggc gacgcagggt catctgcgga ttcacctcat
     2641 gcttcggctt gacatatccc ggcgtaaatt cagaggtgga gccgccacgg ga
//
LOCUS       DAZUGK010000335         2666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZUGK010000335 DAZUGK010000000
VERSION     DAZUGK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.337"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            91..579
                     /gene="eco"
                     /locus_tag="ACHY5W_004686"
     CDS             91..579
                     /gene="eco"
                     /locus_tag="ACHY5W_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFS4449419.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(955..2601)
                     /gene="mqo"
                     /locus_tag="ACHY5W_004687"
     CDS             complement(955..2601)
                     /gene="mqo"
                     /locus_tag="ACHY5W_004687"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFS4449420.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVNDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          623 a          742 c          672 g          629 t
ORIGIN      
        1 ttcataaagc aaattaataa atctgatgaa tatgttaacc ttcagcgaca tcatcggtgc
       61 aaacctataa atgaagaagg aaagcaaaaa atgaagacca ttctacctgc agtattgttt
      121 gccgctttcg ccaccacttc cgcctgggcg gcagagagcg tccagccact ggaaaaaatc
      181 gcgccttatc cacaagctga aaaaggcatg aagcgtcagg tgattcagtt aaccccgcaa
      241 gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga agtcgattgc
      301 aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg gggctatgat
      361 tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg cccggatggc
      421 aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct gcgttacaac
      481 agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta ccgcgtctgg
      541 aaggcggaag agaaaattga caacgcggta gttcgctaaa ctgccgtgaa gtgcggcacc
      601 ccgtaggttg gataaagcgg tcacgccgca tccgacatct aacgcccgag ccggttgcct
      661 gatgcgacgc tggcgcgtgt tatcaggcct acaccgctgt gaagtgctcc accccgtagg
      721 tcggataagg cggttacgcc gcatccgaca tccaacgtcc gagccggttg cctgatgcga
      781 cgctgacgcg tcttatcagg cctacaccgc tgtgaagtgc ggcaccccat aggttggata
      841 aggcggttac gccgcatcca acatctaacg cccgagccgg ttgcctgatg cgacgctggc
      901 gcgtcttatc agcatacgcc acatccggca taccatgccg gatgtggcac atcattacaa
      961 cgcaatatcc gccacttctt tttgaacggg ttgcggtttc agctgcggtt tcggcgtact
     1021 atctgctgct tgcggtttgt cgtatttcag ccccagcact tcgctggtgt actgcaactc
     1081 gcgttctgtt gcggctacat caccgttcag cttgcgtcca taagacggaa cgatcgcttt
     1141 caacgtagcc tgccattgcg ggctggaaac gcgatcgcca aatacttttt ccagcagatt
     1201 caacataatc ggcgcggcgg ttgacgcccc tggcgatgcc cccaggagcg cggcaatggt
     1261 tccttgctgg tcattgacca cttcagtacc cagacgcagt acaccacctt tatcggcatc
     1321 acgcttgata atctgcacac gctgccccgc ttgccacaaa cgccagtcct cttttttcgc
     1381 ttgcggatag tactctttca acgcttcaaa acgatcctct tcactcaaca tcacctgact
     1441 caccagatat ttcaccagat cgaaattatc cagcccgacg tgcatcatcg gcatcacgtt
     1501 agaggtggtg gtggaactca tcagatccca caatgaaccg tttttgagga atttggttga
     1561 gaaggtggca aatggcccaa acagcactac gcgtttaccg tccagaacgc gggtatcgat
     1621 atgcggaacc gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct tcgccagatg
     1681 gtgattaacc acgtccgggt tttccgaaac aaggaactgt ccgcccaccg ggaaaccggc
     1741 gtaatctttc gcttccggaa tccccgattc ctgtaacagc ttcagcgccg caccgcccgc
     1801 gccgataaag acaaattttg cacgaatgtt ctgtgcagtg ccatttttta gatcggcaac
     1861 ggtaacggtc caggtgttat cgtcattacg ctttagggcg cggacttcac tgctgagttg
     1921 cagcgagaag ttagatttct tctgcaagga agcaattaac tggcgggtga tctcgccgta
     1981 gttcacatcg gtaccaattt ccgtacgcgt ggctgccact ttctgttgcg gatcgcgccc
     2041 ttccatcact aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc
     2101 gcgaaacagc gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat taacgttatc
     2161 ctcgccccag acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag
     2221 cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg cttcgttaat
     2281 ggcgactgct ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt agttcagttc
     2341 catcagtgca gaatgcccgg ttccggcgtt attccagccg ttcgaactct cctgcgcgac
     2401 accctccagg cgctccacca tggtcatcga ccattcaggc tccagctcgc gtaaataggt
     2461 ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc
     2521 ggacgctttc gcttttgccg ccatcgaaac ggcattaagc cccacggcca tcgagaagag
     2581 catggcagtc acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg
     2641 agatttgcgc ggcatcaacg gtaaca
//
LOCUS       DAZUGK010000336         2662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZUGK010000336 DAZUGK010000000
VERSION     DAZUGK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.338"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..1431
                     /locus_tag="ACHY5W_004688"
     CDS             37..1431
                     /locus_tag="ACHY5W_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFS4449421.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(1472..1786)
                     /gene="rhaM"
                     /locus_tag="ACHY5W_004689"
     CDS             complement(1472..1786)
                     /gene="rhaM"
                     /locus_tag="ACHY5W_004689"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4449422.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(1796..2620)
                     /gene="rhaD"
                     /locus_tag="ACHY5W_004690"
     CDS             complement(1796..2620)
                     /gene="rhaD"
                     /locus_tag="ACHY5W_004690"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4449423.1"
                     /translation="MQNITQSWFVQGMIKATSDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHTQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
BASE COUNT          674 a          599 c          709 g          680 t
ORIGIN      
        1 agcgtagggc ctcctttatt tcagggaacg taaataatga aaaaatcgac attatcttta
       61 gccatcggtt tattattggc atgtagtacc ggtatggcaa aaacacagca tttaacgctg
      121 gaacaacgca tggcattgct ggaagaacgc ctggaagcgg cagaaatgcg ggcggcaaaa
      181 gcagagagcc aggttaaaca gctgcagaca caacaagccg ctgagatccg cgaaattaag
      241 gctgcccagg gcaatacgcc ggtaaatgga cagacaacag cggaatctgc aaagaaaaat
      301 gccacctcac ctaatctttt gctttcaggt tatggcgatt taaaaatcta cggcgatgta
      361 gaatttaata tggatgcgga aagtaatcat ggcctgctgg caatgaccaa cgctgatgtg
      421 aatagcgatc ccactaatga gcagtggaat ctcaatggtc gtattctgtt aggttttgat
      481 ggtatgcgaa aactggataa tggctatttc gccgggttct ccgcacaacc gctgggggat
      541 atgcacggtt cagtaaatat cgatgatgcg gtattcttct ttggcaaaga aaacgactgg
      601 aaggtcaaag taggccgttt tgaagcctac gatatgttcc cgctgaatca ggataccttt
      661 gttgaacatt ccggtaatac tgcgaacgat ctttatgacg atggcagcgg ttatatctat
      721 atgatgaaag agggccgcgg gcgctctaac gctggcggta atttcctcgt cagcaaacaa
      781 ctcgataact ggtattttga attaaacacg ttacttgaag acggaacatc tttatataat
      841 gacggtaatt atcatggacg cgatatggag cagcagaaaa atgttgctta tctgcgccca
      901 gtaattgcct ggtcgccgac ggaagaattc accgtttccg cagcgatgga agcgaacgtg
      961 gtaaataatg cttatggtta taccgatagc aagggtaatt ttgtcgatca gtccgatcgt
     1021 accggctatg gtatgagcat gacctggaat ggcctgaaaa cggatccgga caatggcatt
     1081 gtggttaatc ttaataccgc ctatttagat gctaataatg agaaagattt cactgccggg
     1141 attaacgcgc tgtggaaacg tttcgagctc ggttatatct acgcgcacaa taagattgat
     1201 gaattcagcg gtgtagtttg tgataacgac tgctggattg atgatgaagg gacgtacacc
     1261 attcacacca ttcatgcgtc ttatcagttc gctaatgtga tggatatgga gaactttaat
     1321 atttacctcg ggacgtatta ctccattctg gatagcgacg gcgataaaaa acacggtgat
     1381 gatactgatg accgttacgg cgcacgcgtt cgctttaaat acttcttctg atgaagtcgc
     1441 ggtgctggat attttccggc accgcaactc attaaggcag gtaaaacact tcttgcagct
     1501 cgctactcac cgggctgtta tccgggttag cgggcataac atcggtcata tatttccacc
     1561 aacgttggca gatttcagtg ctggctaccg cattccaccg ttcttcagat tcaatctcta
     1621 ccgtggcaaa cagcagatta cgcgctttgt cgaggtaaat ggcgtagtta tgcgcaccgt
     1681 gagatttcaa cactgcttcc agttccggcc agatgggatt atgccgacgc tgatactctt
     1741 cgtgggcgtc ggggtttacc tgcatgacaa aggctttgcg gatcatgttt gctccttaca
     1801 gcgccagcgc actggcgagt ggtgtaacgc cgaaacgctg gccgagcgct atcaactctt
     1861 cacggctgat agtctgtttc atgccgccca tcgaataaac cttcactaat acttgtgctg
     1921 atttttctgc ggtgtcgatt aaaccgaagg tttcatccag cgtcggtccg gaaccgaaca
     1981 caccgtggaa gggccacaac accagcgaat gtttttgcat ctcttgggca gttgcctggc
     2041 cgatttcgtc cgtgccgggt accatccacg gcagaatgcc aacgccatcc gggaatacca
     2101 ccagacactc ggtgctgcct tcccacagtt ggcgagtgaa gaccgcggtg tcgttttcaa
     2161 gtacataggt gagggcgatc aggttggtgg cgtggcagtg catgatcacc cgatctttac
     2221 cgttggtggc tttaatgcgc tcgcagtggg agagaaagtg agccggaagt tcagaagttg
     2281 gaacggcttc gttggttaac ccccagagaa tatggtagcc cgcgccgtcg ctgtcgactt
     2341 ttacgacgcc taagttagcc gcaggatcaa gctggacgtt acggaagaat ttgccggagc
     2401 cggtgacgat aaacggtgta tttgccagta aaggcatggg ttggctgagc gggatatagc
     2461 gcggttgagt gtggaaattg tcgtgatacg gtgcgatatc ggcgtcatcc aggcgtagcg
     2521 tcaggttgcc gccgttgcgc tcgtcccagc ctttgagcca ggcgtcggag gtggctttaa
     2581 tcattccctg gacaaaccag gactgagtaa tgttttgcat gttcggtgtt cctgtaaatt
     2641 cggtgttgtc ggatgcacga cc
//
LOCUS       DAZUGK010000337         2653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZUGK010000337 DAZUGK010000000
VERSION     DAZUGK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.339"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..>2653
                     /locus_tag="ACHY5W_004691"
     CDS             125..>2653
                     /locus_tag="ACHY5W_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFS4449424.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLL"
BASE COUNT          768 a          484 c          584 g          817 t
ORIGIN      
        1 cgctcatgat tgtaacaagt tttttcgaga tataaatgat ttgtctcatt tttagcgagt
       61 tcatcgggtt gagctttttt atgatgatag taacctgtta cgaattgtat caaaggaagg
      121 caggttgctt gtgaaaactc cagttaaccc acttttgcaa tggcttaaca tgttttttag
      181 tcgccgttca ttatccggag ctgatggacg ggcgttatat gcttaccgtt gtactgatac
      241 agagtacgaa agtttggcag aactactgcg tacatatgcc ccccgtagtt atccaagaac
      301 gatattcatt tcctatagcg atgttctatt tagcatatat gccgcagagt ttatccgccg
      361 gactcatacg gtcggacacc ctaaatggga cacaatttta gattctatta actggaaagt
      421 tccgtatgtt catcgacaga agctggttaa tgatggcatt cgctactgga aaagaaagat
      481 aagaaacctg ggacaagctt cgggttatct gcatacactg gcttgtgaag gtggtttgcc
      541 tatccgcatg attgaaaatg agagcggtta tttgattacc tatttcagaa ggatatatca
      601 ggcgcttcgt gggcaatcat ctcaatatcc tgccgctaaa attgcacaag aattaggcga
      661 tacgattcct gtcacaatgc aaaacgaact ggtctatgaa atagccggtg aattctgcga
      721 gactctctgc cgattactta gtgagcatcc tcctcatagc agtgatcccg tttctgcatt
      781 acgtaagctc tctccagact ggcatcttca acttccactc gttctccccg aagcgaatgc
      841 tgcagagatt gtaaggcgat tactttctca atcttctgaa atacgcagtg caagtagtct
      901 tcaggttgaa agaatctggg tcgatgttga tgacagttgg tattgcgatg cccggttccg
      961 attcccggcc acaatgcgta cagaacagtt aacctctttg tttgaatgcc atattcagcc
     1021 ggagcagacc cgactcatca tttcaggaaa atggaaaaat ggcggggcaa ggttggcaat
     1081 gctaagccgt tatgagcagc aagattggcg ggtcgagtta ttacctattg cgatgcaaaa
     1141 actctctggt gcagatgcaa tggccgaaat ctcattgtcg cttcatgaag gtccaattct
     1201 gttaggtcac acaattccaa aaggcggtta tgaacttact gaagaacttc cctgggtttt
     1261 tgaagcgatg aatgagagtg aatcgcagct aaaacttgtt ggtatggggt ctgtgagttc
     1321 caggctaaat gctctgttta tttcgctacc gaaaaatagc catttagata ttagtggaga
     1381 aggtgagttt gatatcccaa ggttgctgaa aaacagtgaa cgcagcttaa ctaaaataag
     1441 tggagtattt agcgttgttt tacatgatgg tgctgtttgt acaattcgca cccagcaact
     1501 ttatgattct gcgattgaat attatattaa atcgacagaa gttgaactgg ttaaatcgga
     1561 ttatcctgtc catcgagcct ggccgaagat cggttggaaa aaggatctgc aatatggcat
     1621 tgtaccggag aaagaacttt tttggcgttc cattcgttca ggtaataatg cctggtattc
     1681 tgtcgcatca gaaatgccaa aaggacagat agaagtccgg cgaatagtta atgatgaggt
     1741 tttatttagc ggtaaagttg tagttttacc agcagacttc gatattaata ttatacctga
     1801 gagtgctcag caaggcatta ttatgctttc tggtataacg gatactagaa ttgataaata
     1861 ttcaaacaat gaaaaagtaa cacttaagtc cgattattca caaaacgagt gtgctattta
     1921 ttataattca tcgctgatgc tggaaaatac cgttgattta cgggtctcct ggaaagatgg
     1981 ttctaatctt aaactattat tacctaagcc ggttagcggt ggccgatttg taactaatga
     2041 tggttctgtt cattttgatg gtgtggcatc tatagcacat ttgcatggaa tagatgctga
     2101 gttattaacc atatcatgtg ctggaagagg atatcttaat atcgagttat tggatgaaaa
     2161 tccagtagct gaaaaattcc gctatttaca tgccgacctt cctcttttat caggacgcaa
     2221 tgacaaatta caacaaattt cactttatga gaactataat ttgctaaatg ctatgctggc
     2281 atgtgcatgg aacagcaata gtacattatg tgttgatttt tactctgaca gatttggaaa
     2341 ggataaagca acactcaata ttaaacgcta cgatggtagt tttattgaac acgatcaagg
     2401 gttactggtt gatataaaaa attctgttgt ttttcctgca aatagaatag acgaactggt
     2461 tgttgatgct atttctctta agaatcctgg cttacacata tcattgttaa aaaaagatga
     2521 gtttgcttat gatctttcag ctctgaatgt tcaggatagt ccatggttaa ttgtgggaaa
     2581 acttgatggt acagctcgca ttgcaccagt aattaaatgg atgctacctg tattgcagac
     2641 aaatgattta tta
//
LOCUS       DAZUGK010000338         2641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZUGK010000338 DAZUGK010000000
VERSION     DAZUGK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.340"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..182)
                     /locus_tag="ACHY5W_004692"
     CDS             complement(<1..182)
                     /locus_tag="ACHY5W_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449425.1"
                     /translation="MSVIYSSAINSLVVINTGLSTCWLFRQELLVCHVNRKREKDMLK
                     QQDMTETARVVFDELSV"
     gene            complement(303..884)
                     /locus_tag="ACHY5W_004693"
     CDS             complement(303..884)
                     /locus_tag="ACHY5W_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS4449426.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGAGVKNIQNNYARN
                     AERFIEGKHFFKVAGDALKNLRVALNYSQNLQPSLRGLQISPKARSLILWTERGAARH
                     AKMLETDQAWDVFEKLEDCYFRQKDPSAPVSCQKSYDTRVLCYQRGGVTVSTIQLRDD
                     DIVISLESWLELARANGWFVVRRDKLVERLMQL"
     gene            complement(1158..1712)
                     /locus_tag="ACHY5W_004694"
     CDS             complement(1158..1712)
                     /locus_tag="ACHY5W_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4449427.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLVHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMSKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGYLKTVC"
     gene            complement(1709..1999)
                     /locus_tag="ACHY5W_004695"
     CDS             complement(1709..1999)
                     /locus_tag="ACHY5W_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS4449428.1"
                     /translation="MVDLRKAARGQMCQVRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTGDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1999..2598)
                     /locus_tag="ACHY5W_004696"
     CDS             complement(1999..2598)
                     /locus_tag="ACHY5W_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS4449429.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLYQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPREREMVFGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTQDMALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKAVLNVLWNWILFRKFSSLEEVENVAAQLLEFA"
BASE COUNT          668 a          689 c          577 g          707 t
ORIGIN      
        1 acgcttaatt catcaaacac aactctggcg gtttctgtca tatcctgctg ttttagcatg
       61 tctttttccc ttttccggtt aacgtgacat accaataact cttgtcgaaa aagccagcaa
      121 gttgaaagac cggtattaat aactaccagc gaatttattg cgctgctgta tattacggac
      181 acaaaaaaac caccttccgg tggcttcctt gtgcgaaaaa aacttgcgtt tcgcctcgcg
      241 atacagcttt gcgaagcata caggaattga agcagtttat gtgcaaaaat gcaagaactt
      301 ttttaaagct gcatcaacct ttccaccagt ttatctctgc gaacaacaaa ccaaccattg
      361 gctctcgcca gttccagcca tgactcaagg gaaataacaa tatcatcatc ccgtaactga
      421 attgtggaaa cagtgacacc gcctctctga taacagagaa ctcgcgtgtc gtaacttttc
      481 tggcatgaaa ctggcgctga cggatccttt tgtctgaaat agcagtcttc cagcttttcg
      541 aacacatccc acgcctgatc ggtttccagc attttggcat gacgggctgc tccgcgttct
      601 gtccagagga tgagggagcg ggctttcggg gaaatttgta accctcttaa agatggttgc
      661 aaattttgtg agtagtttaa agctacccgc aaatttttca gggcatcgcc agccactttg
      721 aaaaaatgct ttccttcaat aaagcgctca gcatttctgg cgtagttatt ttgaatgttc
      781 ttaactccag caccgtacaa ctgcgccaaa agttcggtgg taataacagg aatctggtta
      841 tgggtgatcg gggagagagt ttcaacagaa atttgagttg tcataatgac gccctctggt
      901 ggtttctaaa ccatcaccac cgtcaggttc ctaatcatcg ggtggtgaga cgtacagggt
      961 taggaactac cgggaaacca accggcgagc ttttcagctc ccccatacgc cccaccataa
     1021 ttcagatgtg cgcgtgcata cgacaataaa aaacacgctc gcggcgtgtg tctgtcgcgg
     1081 tctctatccg gggttcctaa tcccgacgcc agattttgct ggcgcgtgag gaatatagcc
     1141 ccggataaca gattgagtca acagacagtt tttaaatacc cggaagagaa tgcatcacgc
     1201 atcggcagat agagcatata ttcagcaaga tttagccata catctacccg actgcagcac
     1261 gtccgaaaac accactcagg gtgtgcatca tttagcaatt cagccatttt gcgcttactc
     1321 atcccccgcc cttcatatct ttgccggagg atacaaatca atcctgggtg ttctgccagc
     1381 acttcactaa tcacccgatc aatgcataac gcctctgcat cagtacaatg caccagccag
     1441 cttttttgct tgccgttgat catatcccgc aaaaaagcct caagttcagg tttgtccaga
     1501 cctgcttttt tcatcctccg gagcgcctca ttaattgccg tttttgttag ctttttagag
     1561 gtcagcaact ggttaaacat atttcccgtc ttaccgccgc caatatacga ccagcgcccc
     1621 cacatacgta gttttccctg aatccagaca ctttccagcg tggtgagacg aaggtgttct
     1681 ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc ctcctttctc cagatttctt
     1741 gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc ttctttggtg taatcgccgg
     1801 tttttacccg cccgtcgatt aaatcgtggc acgagctaca ggcaatcgct gcctgcatat
     1861 cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg gtaatgcgcc agcacagacg
     1921 tttcgggatt gtgattgcag tagccaggga ttctgacctg gcacatctgg ccccgcgctg
     1981 ctttacgtaa atccaccatt acgcaaactc cagcagctgc gcggccacat tttcgacttc
     2041 ctccaaagag gagaatttac ggaacaggat ccagttccac aggacgttaa gtacggcttt
     2101 ataaacctgt tgaaattcag tttcgtccat attcgcaaaa gcaatggact tcgcccgacg
     2161 cccgcggcta ccgtctggat aaaaatgctc ggtgtagaag ccggcctgaa tggtcaccca
     2221 ctcgcggaaa gcgtcaaatg atttgagcaa tgccatatcc tgggttctgc gtgtcgcaac
     2281 ggtattcaga tattgttccg cggcatcact cagagctggc gtatgttccc tgcctactga
     2341 ttcacacagg taatcaacga agccaaacac catttctcgt tcgcgaggtg tgatcgcccc
     2401 accgatcgga gtccagtaat cgaatccgag ttgcagaagt ttaaaaaaac gcttatggaa
     2461 tgcgtagtta cgaacgcgct taaaatcagc gtgtatccac tcgcctattt tgatttgata
     2521 cagaaaatcg caactctccg gcgtcgccgg gagaagtaaa ccagaagaag tttgtttgac
     2581 cagttgtata tgcgccatcg tagttctccg ctggcgcagt agaatgggtg ttcagcccgt
     2641 t
//
LOCUS       DAZUGK010000339         2639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZUGK010000339 DAZUGK010000000
VERSION     DAZUGK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.341"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..626)
                     /locus_tag="ACHY5W_004697"
     CDS             complement(45..626)
                     /locus_tag="ACHY5W_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4449430.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
     gene            complement(623..1366)
                     /locus_tag="ACHY5W_004698"
     CDS             complement(623..1366)
                     /locus_tag="ACHY5W_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4449431.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGQPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFCRVSLSRAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            complement(1377..2075)
                     /locus_tag="ACHY5W_004699"
     CDS             complement(1377..2075)
                     /locus_tag="ACHY5W_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449432.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(2075..2404)
                     /locus_tag="ACHY5W_004700"
     CDS             complement(2075..2404)
                     /locus_tag="ACHY5W_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449433.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(2401..>2639)
                     /locus_tag="ACHY5W_004701"
     CDS             complement(2401..>2639)
                     /locus_tag="ACHY5W_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4449434.1"
                     /translation="RGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAG
                     PQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          543 a          913 c          635 g          548 t
ORIGIN      
        1 ctcccgcagt tcctgtccgt gagcactgcg ggattttttc gcttttatgc ctgccgcccg
       61 ataaccacga cctttccgcc cccgccttca tcacgggtac tgatgtcctg ggatatacgg
      121 cgggagccaa ccagcatttc cccgtaaggc accggcatcg ggttaccctg ggcaatcatg
      181 ttatccagcg acgaaaagta cgtgttctgt ttaccgttat ccgttgccct gtattccggt
      241 gtttttggct tcggggccag catctgtgcc acaccaccca gtatcatgct ggcacccagt
      301 gaaaacagca tcgtggtggc agaaaaaccg ccggcactca gcgctgcacc ccaggctgcc
      361 atcgatgctc cggccgtgaa gaaagagccc acgatggctg ccgcccccag cacaatctgc
      421 agtccaccct ttccggcccc ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc
      481 agctgttcgt gaagacgggc gtacaccgcc tccggtgccg tgtcatcacc ggcaatacgt
      541 atctggtacc agccttcgtt catctgacgg cggaatccgg gcacctgtaa cgacagcgcc
      601 cggatggctt ccgctgccgt gttcacatac aggctgaggc ggcggccaaa tcgttgcaaa
      661 tccccgtgaa ggcagatgcg tgccagtggc ggtgacgcca gacagaatgc gttcgtcgtt
      721 gccatttttc agaatacctc tcccgtttac tcagttgttc aggcagatgg tgaagcagtt
      781 caccgttgcc gcagtaaatg gcggcatgat tggccaccga tgcgccaaag cagcacagca
      841 ggatatcgcc cgcctgtgca cgggacaggg acacccggca aaagccggtg gcctccatat
      901 tgtccaggta caggttctga ccgttgcgcc accagtcatc ctcacgctca aaatccggca
      961 tatcaattcc cgccagatgg tatgcatccc ggaacagcgt gtaacagtcc gtcaccccgt
     1021 gctcaaagcg ccgtcctgtc agatgtggca cacagcggaa tttatgaatt tccccccggc
     1081 agaccagcca ccaggacagt gcacttttta tctgcagccg ccggtcggcc tcgctcagcc
     1141 agggctgacc accgggatga ctgtggacca gtgccacaat ctccccctgc atctctgccc
     1201 gcagccagtc ttccggtgca atacgaaaat acgcctccgg ctctgcagag atattcacac
     1261 aagggatata ccgctccccc tccggcgttc tcaccacgaa gccgcacgac tccgcaggcg
     1321 cacaccgccg ggtgtgtgcc agaatcgctg attcagtctg tgtcataaac cgggatttac
     1381 tgcgaaagtt tattaatgga aaggaaaccg ccaaaattag ccaccatgcc gcgcatctca
     1441 cacccgcgca tgcacttgct gcatctgtcc ttacggatat ccgtggtggg gttgtcgaac
     1501 tcatccgcca ccgcaggacc gttatacccg cattcatcag agcggtaggt ccacatacag
     1561 gtgttcgcca gcatgatgcg accaggaaac agcgccccgt ccgtctccgt cggtgttgcc
     1621 agcacaaacg aggctgtcat ggccgtcagc tctgacatct gctccaccac ccagcggtcg
     1681 ctcagctcct gctccgggtc cgcttccgga ttgcccgcca caaaattcac cgcatccaga
     1741 aaacgggcat acacccggcg gcggaccacc gtggccccca ccaggctctg caggtcctcc
     1801 gccattccgg tgaccagacc gaacagattc gacaccgtca gcgacgggcg ggcactgctg
     1861 cccttcccgt tcatctcaaa accgctgccg tcaatcgggt atgcctgata ttcccgcccc
     1921 tgccaggtaa ccgcctcccc tttttcattc agctcattgc agaaaaaata ccgctcacca
     1981 ccctgcaccg tcaggtcgat ttcccagagt accacccgcg gtgactgctc tgatttaacc
     2041 gactcgttca gactttcttc gtgaatatcc tgcatcagtt caccacctgc ttaaactccg
     2101 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag gtcaccttta
     2161 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagcctccg tgctctgcca
     2221 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcaca cggtatgttt
     2281 tcaggtcagc attcagccct gccgccatac gctgtgagta cccatcacca aaacgcactt
     2341 cacgcaccga tggctgcgag ttcacctcca tatccggctt cactttccag cgaaaggttt
     2401 tcatcgtccg ctccctgata acataccgcc atcacgcaac tgcagccgga gttcatcctg
     2461 cgcccccttg cgggccatgt catacaccgc cttcatcagc tgcggccccg cctgtccgtt
     2521 gataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatac cttccgcccg
     2581 ccgcatctgc gccggacttc cggcaccacc cacataacca ccttccgcat agccgcgca
//
LOCUS       DAZUGK010000340         2627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZUGK010000340 DAZUGK010000000
VERSION     DAZUGK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.342"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..519
                     /locus_tag="ACHY5W_004702"
     CDS             <1..519
                     /locus_tag="ACHY5W_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449435.1"
                     /translation="YSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSP
                     DVKNREIKLEWSGDKRDLPTAEAEISHVQASIIKWYASEYHNGRQVLDEIQTPSAINS
                     ELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAIYLALGFFISIVISVMFCLVK
                     KMVDEYQQNSGQ"
     gene            complement(760..1455)
                     /locus_tag="ACHY5W_004703"
     CDS             complement(760..1455)
                     /locus_tag="ACHY5W_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFS4449436.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANEWLTELNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1448..>2627)
                     /locus_tag="ACHY5W_004704"
     CDS             complement(1448..>2627)
                     /locus_tag="ACHY5W_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFS4449437.1"
                     /translation="VYFAKTKEDATRYILQVAQRKNARRVVKSKSMVTEEIGVNHVLQ
                     DAGIQVIETDLGEYILQLDQDPPSHVVVPAIHKDRHQICRVLHERLGYEGPETPEAMT
                     LFIRQKIREDFLSAEIGITGCNFAVAETGSVCLVTNEGNARMCTTLPKTHIAVMGMER
                     IAPTFAEVDVLITMLARSAVGARLTGYNTWLTGPREAGHVDGPEEFHLVIVDNGRSEV
                     LASEFRDVLRCIRCGACMNTCPAYRHIGGHGYGSIYPGPIGAVISPLLGGYKDFKDLP
                     YACSLCTACDNVCPVRIPLSKLILRHRRVMAEKGITAKAEQRAIKMFAYANSHPGLWK
                     VGMMAGAHAASWFINGGKTPLKFGAISDWMEARDLPEADGESFRSWFKKHQAQEKKNG
                     "
BASE COUNT          706 a          693 c          571 g          657 t
ORIGIN      
        1 tacagcctaa aaataaacca gacaacgcca ggtatacttg ttaactgtga tagcaataat
       61 aactttgcct gtcagaccac aatgactgaa gatgttattc agcgaattac tacatttttt
      121 cacaccagcc cagatgtcaa gaacagagaa ataaagctgg aatggtcagg agataagaga
      181 gatttaccaa ctgctgaagc ggaaatatct cacgtgcagg cgtctattat caaatggtat
      241 gcgtcagaat accataatgg caggcaagtt ctcgatgaga tacaaacgcc ttcagcaatt
      301 aacagtgagc tttatacaaa aatgatatac ctgaccagga actggtcatt gtatccgaac
      361 ggtgatggct gtgtaactat aagctcacca gaaataaaaa ataaataccc tgctgccatt
      421 tacctggctc tgggattttt tataagcatt gtaatttctg taatgttttg ccttgtcaaa
      481 aaaatggtag atgaatacca acaaaactct gggcagtaat aaacagcatt ctgatagaga
      541 gtgcctgtat aaagctgaag tgacaatgac cgggaaggga acccgttttc aggaatggat
      601 gcctgtcttt ttcactgtaa atatcctggc attactaatg ccaggatatt tacagaagag
      661 gagcataacc atgacagaac ggggttaagt caggttgtcc ttagcgctat tcgcaacctc
      721 gtgccgcctt caggtttatg tccagacttc atatctctct cagcaatcct caataatcag
      781 atacaccgct ttcaccgggc catgaacccc gacgactttg ataagctcaa tatccgccgt
      841 tgaactaggg ccgctaatga tgttaatgca ggacggcatg cgttcaccgg cctgcgcttt
      901 ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt tacgcaggat
      961 aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg cggaaaaaag
     1021 aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca ctttagcctg
     1081 ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg cattgcattc
     1141 ctgctgcaaa cgttcgctaa tccccaattc ctccagcctc gtgtcaccgc taatcacgac
     1201 cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct tcgcctcgct
     1261 ggtcagctca cagcgcgtca acataacatc gctggcaaac tggataaacg cgtcacagcg
     1321 ctgctgttgg ttaagttcgg taagccactc gttagcatag ttattaagcg gcgcatcttc
     1381 tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat tcaaaaattc
     1441 gcctcgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa ctacggaaac
     1501 tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc gcgccaaatt
     1561 tgagtggtgt tttgccgcca ttgataaacc agcttgccgc atgagcgccg gccatcatcc
     1621 cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc gcccgttgct
     1681 ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa atcagttttg
     1741 acagcggaat acgcactgga cacacgttgt cacaagctgt gcataaagag caggcgtagg
     1801 gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca ccaattggcc
     1861 ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga caagtattca
     1921 tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc agcacctcag
     1981 aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg tgccccgctt
     2041 cgcgcggtcc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg gcactgcgcg
     2101 ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc tccattccca
     2161 ttactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct tcattggtca
     2221 ccaggcatac cgaaccggtc tctgctaccg cgaaattaca gccggtaata cctatttcag
     2281 cactgaggaa atcttcgcgg attttttgcc ggatgaataa ggtcatcgct tcaggcgttt
     2341 ccggcccctc atagcccaga cgttcgtgta gcactcgaca gatctgatgg cgatctttat
     2401 gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga atatattcac
     2461 ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg acaccaatct
     2521 cttcggtcac catcgattta gatttcacca ccctccgggc atttttgcgt tgggcaacct
     2581 gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacg
//
LOCUS       DAZUGK010000341         2605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZUGK010000341 DAZUGK010000000
VERSION     DAZUGK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.343"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..736
                     /gene="tnpA"
                     /locus_tag="ACHY5W_004705"
     CDS             62..736
                     /gene="tnpA"
                     /locus_tag="ACHY5W_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFS4449438.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            733..1080
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004706"
     CDS             733..1080
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449439.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1100..2605
                     /locus_tag="ACHY5W_004707"
                     /pseudo
     CDS             1100..2605
                     /locus_tag="ACHY5W_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          660 a          706 c          721 g          518 t
ORIGIN      
        1 tcttgtgaaa attgtcctgt ctggcaacaa tcgcgcccat ctatatagat ggacacgaac
       61 gatgaattcc cagacaaaaa aagatattcc ctgcttccgt tcttatttgc ctgatgctct
      121 gcgtttaaga tttgaagata aactgaccat ccgggccatc gctcagcgtc taggtctcag
      181 tcattccaca atacatacgc tttttcagcg atttattgca tccggtatcg catggccatt
      241 gcccgattca gtttcattag ctcagcttga tgccatcctt tatgccaaca gaaagaagga
      301 attaacagag cctcaaatca gcgaaggcac atggcgaaaa gaacggcgag ccagctacag
      361 ccgtgaattt aaggtccgtc tggctaagca ggcattacag cccggtgctg ttgttgcccg
      421 gatcgccaga gagcacggta tcaatgataa cctgctgttt aaatggaaaa gccagtacga
      481 ggacggctta ctgagcgatg atgacataca ggaatgcatg cctgtcccgg tggctctgac
      541 tgatacgcca gagccgacca gaccagttac aaatcccttc tggcgtaaca agcctgatga
      601 gtgccctgag agtgatcccg gaaacgtccc acggtgcgag ctgcatctta aatcaggtgt
      661 ggtaaaactg tttgaccctc tcactccgga aatgttacgg gcgctaatcc gcgaaatgaa
      721 aggaggtacc cgatgataac gctgccaacc ggtaccagaa tctggatcat cgctggcatc
      781 acagatatgc gttgtggctt caacggcctg gcttcgaagg tgcagaacac gctgaaagat
      841 gacccgttct ccgggcatat cttcgtcttc cggggccgca gtggcaaaat ggtgaaaata
      901 ctgtgggccg atcgtgacgg gttatgcctg ttcgccaaac gcctggaacg gggccgcttc
      961 gtctggccgg taacccggga agggaaagtg cacctgacgc cagctcagtt atccatgcta
     1021 ctggagggga tcgcgtggca acatcccaaa cggacagaac ggcctggcat ccggatataa
     1081 cccgtgataa aacaggggaa tgaacaacac actccccgac gacatcgagc aactgaaggc
     1141 cctgctgatc gcacagcagg ctgttatcgt ctgtctggtg aaataaccgg ctatgcccgc
     1201 gagatcagct cactcagagc gctggtcgct aaactgcaga gaatgttgtt cggtcgcagc
     1261 agcgagaaaa gccgcgagaa gatagaaaag aagatcgcac gggcagaaac gcgtataacc
     1321 gagctccaga acaggcttgg tgaggcgcag ttgcaactca cctcaatggc cggagagaca
     1381 gcgccgaaaa catcagactc tcccgtccgc aaagcacttc cggcaacact tccccgtgac
     1441 aggcaggtta tctccccggc agaaaccgaa tgccccgtct gcagcggcaa actgaaaccg
     1501 ctgggagaaa gcatctctga acaactggat atcatcaaca ccgcgttcag ggtaatcgaa
     1561 acggttcgcc caaaactggc ctgcagccgg tgcgactgta tagttcaggc tccgcagcca
     1621 ccaaaaccca tcgagcgcag ttacgccagt ccggctctgc tggcccgcat aatcatggct
     1681 aagttcgccg agcatctgcc gctgtaccgt cagtcggaaa tctatgcccg ccagggcgtg
     1741 gagctgcacc gcaatacgat ggggcgctgg gttgacatca tgggagagca gcttcgcccg
     1801 ctgtatgatg aactgaagca ctatgtgctg atggcgggta aagtgcatgc cgatgacacg
     1861 ccggtaaatg tactggagcc gggtcagggt aaaacccgta ccggacggct gtgggtctat
     1921 gttcgtgacg atcgcaacgc cggttcgacc atgccggcag cggtgtggtt ctcatactct
     1981 cccgaccgca aaggcatcca cccacagcaa catctggcgg actacagagg tatcctgcag
     2041 gccgatgcat atgcgggtta caatgctctt tacgaaagcg gtcaggtaac cgaagcggct
     2101 tgtatggcac atgcccgacg caagatccac gatgtacatg tccgccatcc aacgacagta
     2161 acgggagaag cgctccgtcg tatcggggaa ctgtacgcta tcgaggctga gatccgcggc
     2221 agtccggcag aagagcgact ggcggtcaga aaagccagaa cggtaccgct aatgcagtcg
     2281 ttgtatgagt ggctccaggg gcagatgagc acgctgtcgc gccactcgga tacagcgaaa
     2341 gcgttcacct atctgctgaa gcaatgggac gctctgaacg aatactgccg caatggctgg
     2401 gtggagatcg acaataacct gtgtgaaaac gccctccggg tagttgcact ggggcggcgt
     2461 aactacatgt tcttcggctc tgatggtgga ggcgagagtg cggcagtgat gtacagcctg
     2521 atcggtagct gcaaacttaa cggaatcgag ccggaaacgt ggctacgcca cgtgatcagt
     2581 gtaatcaaca cctggcctgc caacc
//
LOCUS       DAZUGK010000342         2599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZUGK010000342 DAZUGK010000000
VERSION     DAZUGK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.344"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1421
                     /gene="zorA2"
                     /locus_tag="ACHY5W_004708"
     CDS             <1..1421
                     /gene="zorA2"
                     /locus_tag="ACHY5W_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFS4449440.1"
                     /translation="TSIPVLLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRSALAE
                     NNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLIAQIKLLRTDISDSSQAQLAN
                     HTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQNLTEQFGENFKALDASVK
                     KLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVN
                     QHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQIL
                     LNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGE
                     EFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKAS
                     AGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKL
                     IKDYQIVMGQLQALISANKHRG"
     gene            1428..2135
                     /gene="zorB2"
                     /locus_tag="ACHY5W_004709"
     CDS             1428..2135
                     /gene="zorB2"
                     /locus_tag="ACHY5W_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFS4449441.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            2139..>2599
                     /locus_tag="ACHY5W_004710"
     CDS             2139..>2599
                     /locus_tag="ACHY5W_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4449442.1"
                     /translation="MKLSIDLSELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQR"
BASE COUNT          871 a          472 c          529 g          727 t
ORIGIN      
        1 ataccagcat ccccgtatta ttaggtggcc taaaaacagc attcattaca agcattgtag
       61 gtatgttttt tgccatttta tttaatggaa tggatgcttt cttttttgcc aataaacgaa
      121 gtgcgttagc agaaaataac cctgaatctg ttacacctga acatatctat catgaattaa
      181 aagagcagaa ccagactctg actaaattag tctcgggtat taacggtgat agtgaaggtt
      241 ctcttattgc tcaaataaaa ttactacgta ctgatattag cgattcctcg caggcacaat
      301 tagctaatca cactcatttc agtaataagc tttgggaaca acttgaacaa tttgcagatc
      361 taatggcaaa aggtgctaca gaacaaatta ttgatgcttt gcgacaagtc attattgatt
      421 ttaatcaaaa tttaactgaa cagtttggtg aaaactttaa agctcttgat gcctctgtaa
      481 aaaaacttgt tgagtggcag ggaaattata aaacgcaaat tgagcagatg tcagaacaat
      541 atcaacaaag tgtcgagtcc ctggttgaaa caaaaactgc ggttgcaggg atttgggaag
      601 aatgtaaaga aattcctctg gctatgtctg aactgcgtga agtgcttcag gtgaaccaac
      661 atcaaatcag cgaactctcc cgccatttag aaacctttgt cgccatccgc gataaagcta
      721 caaccgtatt acctgaaata cagaacaaaa tggctgaagt gggtgaactg ctgaaatccg
      781 gagctgcaaa tgttagtgca tctcttgagc aaaccagcca gcaaatactt cttaatgcag
      841 attcaatgcg cgttgccctg gatgaaggta ccgaaggatt cagacaatcg gttacccaaa
      901 cacaacaagc atttgcctcg atggcacatg atgtcagcaa ttcctccgaa accctaacca
      961 gcacgttagg tgaaacaatt actgaaatga aacaaagtgg tgaagaattc ctgaaatcac
     1021 tagagtcgca ctcgaaagaa ttgcatagaa atatggaaca aaatacgacg aatgtgattg
     1081 atatgttcag taagactggt gaaaagatta accatcaact atccagtaat gccgataata
     1141 tgtttgattc aatccagaca tcatttgata aggcaagtgc agggctgact tctcaagtca
     1201 gagaatcaat tgaaaaattt gctctatcca tcaacgagca gttacatgct tttgagcaag
     1261 caactgaacg tgaaatgaac cgtgaaatgc aatcattagg taatgctctg ctttcaatca
     1321 gcaaaggttt tgtcggtaac tatgaaaaac ttattaaaga ttaccaaata gttatggggc
     1381 agttacaagc attaatttct gctaataaac atcgcgggta atcgatcatg gataagatta
     1441 tagggaaaca attacctaaa aaagatcaag ataatgaaca ttgggtatcc atgtcagacc
     1501 taatggcagg gctgatgatg gtttttatgt tcatatctat tgcttatatg cactacgtac
     1561 gtattgaaaa agaaaaaatt aaagaagttg ccgtagccta cgagaatgct cagttacagc
     1621 tttataatgc tctggatatt gagtttgcaa aggatttaca agactgggat gcagagatcg
     1681 ataaacagac tctggaggtt cgatttaaat caccggatgt tttatttggc ttaggaagca
     1741 cagagctaaa accaaagttt aaactcattc ttgacgactt ctttcctcgc tacctaaaag
     1801 ttctagataa ttatcaggaa catattactg aagtccgcat tgaaggtcac acaagtactg
     1861 actggacagg aacaacgaat cctgatattg cttattttaa taatatggca ctatcgcaag
     1921 gtcgtacacg tgcagtatta caatacgttt atgacataaa aaatatcgcg acacaccaac
     1981 aatgggttaa aagtaaattt gccgcagtag gttattcatc tgcacatccc attcttgata
     2041 aaaccggcaa ggaggatcct aatcgctctc gtcgtgtcac cttcaaagtt gtaacaaatg
     2101 ccgagttgca gattagaaag attattcagg agtaagagat gaaattatct atcgaccttt
     2161 cagaacttat tcaattaggg aagaaaatgt taccagaagg agtcgatttt tttctggatg
     2221 aatcccctat tgactttgat cctatagata ttgagttatc cacgggtaaa gaagttagta
     2281 tcgaagatct tgaccctggt agcgggctta tctcttatca tggccgccag gttcttttat
     2341 atattcggga ccattcaggg cgttatgatg cggctatcgt agatggcgaa aaaggaaaac
     2401 gttttcatat tgcctggtgc agaactcttg atgaaatgcg ccataaaaat cgatttgaaa
     2461 ggtatcatgc aactaaccgc atagatggtt tattcgaaat tgatgatggt tcaggtcgga
     2521 gccaggatgt tgatttacgg gtatgtatga attgcctcga acgacttaat tataaaggaa
     2581 gtattgataa acaacgaaa
//
LOCUS       DAZUGK010000343         2596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZUGK010000343 DAZUGK010000000
VERSION     DAZUGK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.345"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..582)
                     /locus_tag="ACHY5W_004711"
     CDS             complement(<1..582)
                     /locus_tag="ACHY5W_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449443.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESLQSA"
     gene            complement(734..1333)
                     /locus_tag="ACHY5W_004712"
     CDS             complement(734..1333)
                     /locus_tag="ACHY5W_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS4449444.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1401..>2596)
                     /locus_tag="ACHY5W_004713"
     CDS             complement(1401..>2596)
                     /locus_tag="ACHY5W_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4449445.1"
                     /translation="ARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGR
                     ASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNK
                     KLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQ
                     VLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPD
                     GRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSR
                     APERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEI
                     YNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATA
                     GITIS"
BASE COUNT          590 a          762 c          593 g          651 t
ORIGIN      
        1 tgcactttgt aatgactcac tggctttttg agcggccgca gaggccgagg acgaggacgc
       61 ctcctctgac tgctttgcag cggctgcact ttctgccgcc tgccgggctg actccgatgc
      121 atcccctgct gaagtgtcag catttgcagc gctctcttct gcctgactgg ctgatatgcc
      181 ggcattcctc gctgacgtct ccgcctctcc ggcattcttc ttcgcctcct cagcgtgacg
      241 cgccgcttct tccaccatca gttcaaaacg acgcagtgcc tccggccgga cgtcatcctc
      301 cgacatggca ccgagaaaat cattcagcgt ccccggttga gaatcttcat acacggtgat
      361 ggtcccggca tgtgacggag gaaatccctc caccaacaga atgacgctgt actgaccgta
      421 ctcaacatcc atgctgtaac gtccggcttc atccggattt tcagaggcca ccgtgttcac
      481 caccaccgtg ctgctggttc gtctggcctt cagcacaatg gtgcagttct gtactggttt
      541 tcctgtgcca tctttaagca cgccagaaat ttttactgtc atacttttcc accaataaaa
      601 aaagcccgca gcagtgacgc cacgggcttc aggacagtgt aactttacgt ttcctcaaac
      661 gcagttcacc ccataaggtg gatgaacctg cgtatcataa caatatttac agaagataaa
      721 tcggcgtctg ttgtcagaaa cggtatccga taccaacaat aaatgcatcc gatcgccagt
      781 cgccactacc ggaaccttca taagcaaggt caatggtcac ggattcggtc gggttaaact
      841 gcacgccagc cccccacgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca
      901 gcacatcgtg cgttttcccc ttgttgtcag ttacgcggag ataatccccg gagaaagtcg
      961 acacacggct gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgta
     1021 cagacggccc cgccatcacg ctgaaccagc ggttacgcac ggaatcttca tgccagcggg
     1081 tatcgctgta gcgcgttttt tgctcatcct cagcattggc ataactgaag gacgtaatca
     1141 gccccagcgc gtccataaac tcataacggt atttcacgtt aatcccgttc agatcatcac
     1201 taccgggaac gttcgtcgag gcatggagat accccgcgct cagcgtggac tgatgttctg
     1261 ctgcactcgc tggcgtagca gcggcgacct gccagactac tgcggacaaa ataacagcac
     1321 ataatttacg cataattacc tctcgctttt ctgcaataaa aaaggcgcca tttctggcgc
     1381 ccgtatctgg gttataaaat tcagctaatc gtgatgcctg cagtggcttt cttcatcacc
     1441 acaaccagca aatcgctgat acttgctgtg ggataccagt tatttaccag ccatgctgac
     1501 accgaaaact ccagtgtcat gtgaccgtga ccggcaggca tatcaataac accactgtaa
     1561 atcagcgtat tatccagcgc ggtacggtta taaatttcag caccgttttt ccgcactatc
     1621 agacggcatg aggagtaaat atcagtatgc tctttctcat gtttagcgcc gctgaatgcc
     1681 accgccggaa taacaatctg ccggtcaaac ggctgatcgt cataaaccct gacggtaatg
     1741 gttcctgatg gccaccgctc cggtgcacgg gagtcccggg ggaaagcttt gcccactgtt
     1801 ttaacgagat cgccttcaat ctggttcgcg gacaattttc ccagaacccg acagttctcg
     1861 ttaatcgtga cgttgttgag cgtcccggag ttcgcattca cgttaccgct gatatcggca
     1921 tttttcgccg tcagccgccc gtccggtgtc agggaaaatg ccggaggatt accgccgctg
     1981 gtaatggtgg gagccgtcag atatttcagg aacacttcat tcataaatat ctgatcgccc
     2041 tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct
     2101 gccgccagca gcacctgact ctgcattcct gctggcgtat tctcaatacc ggcaccgata
     2161 cccgcaatat aaaggcgtcc gtcctgcatc tgctgcagtt tcacggccca catgctgttc
     2221 aggttattat ttgtatcaac ctgaactttc tgtatctgct ggattgccgc actctgattt
     2281 tccagttttt tattgacggt ctgcgtgatt tcattgctga cattcgtaat ggacgtcctg
     2341 atttcagcca ggtccggcgc aagctgaccg ttatcaatct gcgtccacag ctcctgggcc
     2401 agatgtgttt tcccgatttc tcctttgaaa aaatccaggt aaccttccgc atcatcgctc
     2461 gcccgaccga cagcctccac gaatgccgat ttgccaacag tattcacact gcggatataa
     2521 aaataataat catggcccgg tttgatattg atactggcag ctatccagta cagcgccgag
     2581 ccaagatagc gggctg
//
LOCUS       DAZUGK010000344         2570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZUGK010000344 DAZUGK010000000
VERSION     DAZUGK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.346"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1486
                     /gene="yihF"
                     /locus_tag="ACHY5W_004714"
     CDS             56..1486
                     /gene="yihF"
                     /locus_tag="ACHY5W_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS4449446.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     IPLNVATEIAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1527..2459)
                     /gene="yihG"
                     /locus_tag="ACHY5W_004715"
     CDS             complement(1527..2459)
                     /gene="yihG"
                     /locus_tag="ACHY5W_004715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS4449447.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPVADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          806 a          532 c          563 g          669 t
ORIGIN      
        1 tatttcagca taagtggata ttcaaagttt tgctgtttta tcagggaata tttatatgat
       61 acgtaagtca gctacaggtg ttattgttgc gttagccgta atctggggtg gtggcacatg
      121 gtacacaggt acgcaaattc agcctggtgt cgaaaaattt attaaagatt ttaacgatgc
      181 taaaaagaaa ggtgaacatg cctacgatat gacgttaagt tataaaaact ttgacaaagg
      241 cttttttaat tcccattttc aaatgcaaat ttcatttgat aacggtgcac ccgatctcaa
      301 tatcaagcca ggccagaaag ttgtatttga tgtggatgtt gagcacggtc cgttgcccat
      361 cacaatgtta atgcatggta atgtcatccc agcactggca gcggcaaaag tgaacttagt
      421 gaataatgaa ctgacacaac cgctatttat cgccgcgaaa aataaatcgc ccgtggaagc
      481 gacattgcga ttcgcgtttg gtggctcatt ctctacgaca ttagatgttg cccctgcaga
      541 gtatggaaag ttttcttttg gtgagggcca gtttactttt aatggtgatg gtagttcatt
      601 gtctaacctg gatattgaag gcaaagtcga agatattgtt ctgcaattat caccaatgaa
      661 caaagtaacg gcaaaaagtt ttaccattga ttctctgaca cgattagaag aaaagaaatt
      721 tccggttggt gaaagcgagt cgaaatttaa tcaggttaat attatcaatc agggggaaga
      781 cgttgcccaa atcgatgctt tcgttgcaaa aaccaggctg gatcgcgtta aagacaaaga
      841 ttatatcaat gtcaatctga cctacgaact tgataagtta acaaaaggga atcagcaact
      901 cggtagtggt gagtggtcat tgattgctga atctattgat ccctcagcag tgcgccaatt
      961 tatcatccag tataacattg cgatgcagaa gcagcttgct gcacaccctg agttagcaaa
     1021 cgatgaagtt gctctgcaag aagtgaatgc tgcattgttc aaagagtatt taccgttatt
     1081 acaacaaagt gagccgacca ttaaacaacc ggtaagatgg aagaacgcac tcggcgaact
     1141 aaatgccaat ctggatatca gtattgccga cccagccaaa tcttcatcat ccacaaacaa
     1201 agatattaaa tcgctcaatt ttgatgtgaa gataccgctt aatgtcgcca cagaaatcgc
     1261 aaaacagctt aatttatctg aaggaatgga tgcggaaaaa gcgcaaaagc aggctgataa
     1321 acaaatcagc gggatgatga cattaggtca gatgtttcag ttaatcacga ttgacaacaa
     1381 taccgcctcg ctgcaactgc gttatacacc gggtaaagtt gtttttaacg gacaggagat
     1441 gagcgaagaa gaatttatgt ctcgtgccgg acgttttgtt cattaaaata ctcactcgcc
     1501 tctgctcatt cagaggcgag caaaatttac ttgtcctgcc gctgtgatga catcaacgac
     1561 gtcagaagcc ggtctttttc ctgccacagt gagttcagcc attgctgaaa atggcgctta
     1621 aagcttttgt cattgatgta gtcgccatgt aactcatcgg caacaggctg caaatccaca
     1681 tgtacgacaa tccgcgtcag tttaccgctt aacatatcga agaacggctg acggttattg
     1741 tccggataac acagcgtaac attaagcagt ttatcgaatt gtttacccag tacatttagc
     1801 gccatcgcaa tgcctgccgc ctttggtggc aacaagtttt gaaaagtgga gtgggtttgc
     1861 agatgttttt cttgcgtgaa acgggagcct tcaacgaaat tgacaatggt ggtgggatgc
     1921 aggcgaaact tttcacaaga acgccgagtg gtttcaacat ctttaccacg tcgctccgga
     1981 tgacgtaata aataagcgcg ggaataacgc ttcataaacg gcatatccag cgcccagcac
     2041 gccaggccaa gaaatggcac ccaggccagc tgttgcttga ggaaatattt attcatcgga
     2101 atgtgcttac gaaacagcac gcacagtacg acaatatctg cccaactacg gtggttacaa
     2161 ataagcagat accagttctt cttacttaac ccttccagcc cgtgaacttc ccattgcagg
     2221 tgtgggttca gatgcagtaa taccgccaga ccttcacacc agcaatacat cataaaatca
     2281 caaaaacgcg aaacctttcg ccaaatgact ggcacaggta gcaatagttt tacaatcccg
     2341 gcaatgatga tcgggacgga gcaaaaaata gtgaccaaaa tggtcaatac gatactcagc
     2401 aaaagggtta tcgcagcgag tattctcgtc ataatgaatt tattcaaaag gttagccata
     2461 gacagtgcgc taaagaagtg caaggcgcag attttagcag aaaacgaccc aaataacgga
     2521 tgatccttaa ggagaaaaat aattcatatc tatccacatt agaaaaaatc
//
LOCUS       DAZUGK010000345         2567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZUGK010000345 DAZUGK010000000
VERSION     DAZUGK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.347"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..491)
                     /locus_tag="ACHY5W_004716"
     CDS             complement(<1..491)
                     /locus_tag="ACHY5W_004716"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4449448.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASG"
     gene            complement(555..2216)
                     /locus_tag="ACHY5W_004717"
     CDS             complement(555..2216)
                     /locus_tag="ACHY5W_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4449449.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(2213..>2567)
                     /locus_tag="ACHY5W_004718"
     CDS             complement(2213..>2567)
                     /locus_tag="ACHY5W_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4449450.1"
                     /translation="SEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYMEDGLPD
                     PVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGDAAKKAANRFAVP
                     PPPRLVVNNDNEESG"
BASE COUNT          537 a          832 c          657 g          541 t
ORIGIN      
        1 ccggacgcgg cagaaaacgg gcggtcgtaa gatttaatgg tccggatggt gcattccgca
       61 ttcgcgggca cggtgacggc agacacctcc atcagttccc agcgcagaaa atgcagtccg
      121 cctccgtcca gaaaggtgta ttcatgggga cggaagccca cggacagccc cctgaccagc
      181 ccggtcttaa tggccgccca gacctcatcc agccgggcag ccagttgcga cggcatatcc
      241 ggtacgggct tcaccagtgt tgccgtgatt tccagccctt cgctgacccg gcgtaccgta
      301 cactgcccca ccgggcggga atggtcatgc tgccagagaa acgggatcgc actgccaaac
      361 tccgcgccct ccggctccag gatgtcacca tcccgatccg gagaaggcgt tgacgcaatc
      421 ccggtgatca cccgttcatc ctcactgaag gatttcaccg tcagcaggga acaggcccgt
      481 ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc ggagcggttc gtgatggtta
      541 cagtgtgaac agggttatat gaaaaaaacc gcatattctt tctttttcgg ttccgggtta
      601 agggacatca gggagaccgc attgaacagc gccatcagcg ggtcaatttt tccccgtcca
      661 ctggcctgtt tggtgataag aatggcgtta cctttaggct ccacccgggc attgccgaca
      721 caccaggcca tcaggggctg gtcaccatgc accagcaccc cttcagccag tttgcgctcg
      781 gtggttttaa tggccccgcc cagtttccag ccctggctta tccccaccac aattccgtcg
      841 gggatcccgg cttccgccag tgaatccaga atctgcccca cccctgacgg gtcaataccg
      901 atatggtcca gtaactcagc ctcatgaatg cgacgcacat attccgccac ttccgccgtg
      961 tcatccccga cacgccggac aatggtcata tctccacagg caacaagatc ctgaaaccgg
     1021 gacgcctcgc tcttccgtcg gaccaccgcg gtttcatgcg cccaggcatg gccccagccc
     1081 agccattcgc gggtctcccg gtcacgccca atcacataca tccccagcag atcatccagc
     1141 cctccgccgt caatccccac cgtcaccaca tcagcacgac gcaggatatc gtccaggctg
     1201 atacaacggc cctgctcttc ccagaaatca gcccccgccc agcggtcaga gcgcagggca
     1261 agaccaattt ccacattggc gtgttttgac atgaaccccc ggaatgtctc ttcaccggct
     1321 tcccgggctt tacggtactc ccggtacaga aaggcctcat ccactgaata gccgagattc
     1381 ggattgacca tggcgaggtt ttccatcagc aggtgagccc cgctttccac catttcagga
     1441 gggtgttcaa atatcaccgg cagaaagtgc ggatcatgaa ttttgccgtc gcgcacatcc
     1501 cgggcgtact gcagtttctg tctgaacacc ccggcgggcg gttcattcga ctgggtggtc
     1561 gtatacacca caaacccttc cgggcgggag gcaaggccgc ctatggcttc acgtaacatg
     1621 tcctccgcct tgcactgctt gccaaacagc cacaactcat caatcagcgt acccacggac
     1681 ttgataccgg acaccgtatt cggatcggct gccaccacct tcagggtggt gtctgtcacc
     1741 ctgtgggtga tggtccggat atgggtctgt acctggcaga ggtcatccag atcatcgtca
     1801 cgtcgtacca tatcccgggc agggttgaag gcgttggccg ccacctccac agtcggggcc
     1861 agaatcgtgt aacccgccgc ctgccgccag ttcagtaaca gcgccgtcat catgatcccc
     1921 gcggccagcg tggacttcga gtttttcttg gggataagga taaaaacttc cttgatatgg
     1981 cgtacaccgg tctgcgcatc gtaggagcca aacagggccg ccaccaggtc aaacacccac
     2041 ggtgcacagg actccccgaa cgtcgggcta ccaggtgcat ccacaatccg cagttgttta
     2101 aaaatcgcca aggcatgtgc ggcctcgtcc ggataaatcg gatccggaat aatcgacagc
     2161 ccctttttca ggcgctctgc ccagtccggg caggctgtgc tccatacagg tatcatccgc
     2221 tttcctcatt atcgttattc accaccagtc ggggtggcgg tggcaccgca aaacggttag
     2281 ccgctttttt cgcggcatca ccttttgccg attttttccc ggtatcccct tttttatggt
     2341 gcgtgaactg cgccagacgc caggccgcat ccagtgccag tttcggatca atgcagaggt
     2401 tttccaccag gatccgcccc atggctttca ccggatcggg aagaccatcc tccatatatt
     2461 caataccagg agacatcacc gcggacggtg gcatctccgg attgttttcg tccggctgtg
     2521 gtattgcagc cgcctcacgg cgacggggtt tatcctcctg ctctgat
//
LOCUS       DAZUGK010000346         2561 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZUGK010000346 DAZUGK010000000
VERSION     DAZUGK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.348"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(79..933)
                     /gene="rclR"
                     /locus_tag="ACHY5W_004719"
     CDS             complement(79..933)
                     /gene="rclR"
                     /locus_tag="ACHY5W_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFS4449451.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1159..2484
                     /gene="rclA"
                     /locus_tag="ACHY5W_004720"
     CDS             1159..2484
                     /gene="rclA"
                     /locus_tag="ACHY5W_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFS4449452.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLIHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPRITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTTDNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGT
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          727 a          571 c          596 g          667 t
ORIGIN      
        1 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
       61 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
      121 ggtacaacca aactcgcgaa caaacgcctt gtgaaaagat gattcactgg catagcctac
      181 tgactcagcg atcaccacaa cagggagcgt ttcccgggaa aacatctggg ccgctatttg
      241 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
      301 ggcaaaactt gcccgggaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
      361 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
      421 gagaatgttt ttctctgtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
      481 tgtagcgcag atctggctac acagtgcatc cactcccggc attgccaatc tggattcctg
      541 ttgtaaaaac gggatcgcct ccctcagcca gttatattct acgctgtggt taaccggtgc
      601 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcagccgaag
      661 agtgccgcag acaatacagg ttgattcatt atcgacatga ctcaggcgat gggctgaatt
      721 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
      781 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
      841 atgtggaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
      901 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
      961 caaacaccaa caatcttagt caataactaa tctaatacca tcaaatagtt aatcatgatt
     1021 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
     1081 cgttgtgaag aaatgttatt tgctcttttg cgtctatagt catgatgtca aatgaacgcg
     1141 tttcgacagg aaatcatcat gaataaatat caggcagtga ttattggttt tggcaaggct
     1201 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
     1261 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gtatcccaac caaaacattg
     1321 attcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
     1381 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
     1441 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga
     1501 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
     1561 ccaattccta gaattaccac cacgccagga gtatatgaca gcaccggatt acttaatcta
     1621 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
     1681 tctatgttcg ctaattttgg cagcaaagta accattttag aagcagcttc gctgtttttg
     1741 cctcgggaag aacgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
     1801 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
     1861 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
     1921 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
     1981 gtcgttgaca agcgattaca taccaccaca gacaatattt gggcgatggg agatgttacc
     2041 ggcgggctgc aatttactta catatcactg gatgattacc gcattgtacg tgatgagtta
     2101 ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg tgccttattc cgtatttatg
     2161 acaccgcccc tgtccagggt tggtatgaca gaagaacaag ccagagagag tggtgctgat
     2221 attcaggtgg tgacattgcc tgtagctgca attccgcgcg ccagagtgat gaacgatacc
     2281 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg gacatcactg
     2341 ctgtgtgttg actcccacga gatgattaat atagtgaaaa tggtgatgga tgccgggctg
     2401 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
     2461 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
     2521 ctcagagaaa agaatcttat taattccctg ttactctata a
//
LOCUS       DAZUGK010000347         2553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZUGK010000347 DAZUGK010000000
VERSION     DAZUGK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.349"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..1692
                     /gene="mppA"
                     /locus_tag="ACHY5W_004721"
     CDS             79..1692
                     /gene="mppA"
                     /locus_tag="ACHY5W_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFS4449453.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1743..>2553)
                     /gene="ynaI"
                     /locus_tag="ACHY5W_004722"
     CDS             complement(1743..>2553)
                     /gene="ynaI"
                     /locus_tag="ACHY5W_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFS4449454.1"
                     /translation="PGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIM
                     SRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFD
                     RPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTN
                     RRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYC
                     FTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          734 a          580 c          620 g          619 t
ORIGIN      
        1 tgatggaagt tgtttgcatt tctttaaggc gaaacaaata attacgcatc aattttaatg
       61 tcggttagag ggaaacttat gaagcactct gtttcagtca cgtgttgtgc gctgttggtc
      121 agcagcattt ctctttcgta tgctgcagaa gttccgagcg gcacagtact ggcagagaag
      181 caggagctgg tgcgccacat taaagatgag cctgcgtcgc tggatcccgc taaagccgtg
      241 ggcctgccag agattcaggt cattcgcgat ctgtttgaag gactggtgaa tcagaacgag
      301 aaaggggaga ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg
      361 acttttaccc tgcgcgataa cgcaaaatgg gcggatggca caccggtaac ggcgcaagat
      421 tttgtctaca gctggcagcg tctggtggac ccgaaaacgc tatcgccatt tgcatggttt
      481 gccgcgctgg cgggaatcaa caacgcacag gcgattattg atggtaaagc tacgcctgac
      541 cagcttggcg tcaccgcagt tgatgcccat actttgaaaa ttcagcttga taaaccgttg
      601 ccgtggtttg tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa agccaacgta
      661 gaaagcggta aagagtggac gaaacccgga aatctgatcg gcaatggcgc ttatgttctt
      721 aaagagcgcg tagtcaatga aaaactggtc gtggtaccga atacccatta ttgggataac
      781 gccaaaacgg tactgcaaaa agtgaccttc ctgccaatta atcaggaatc cgcagccact
      841 aagcgttacc tcgcggggga tattgatatc accgaatcct tcccgaaaaa tatgtatcag
      901 aagctgttga aggatattcc ggggcaggtt tatacgccgc cgcagctcgg gacctattat
      961 tatgcgttta acacgcaaaa agggccgacg gcagatcagc gcgttcgtct ggcattaagt
     1021 atgacgatag atcgccgcct gatgaccgaa aaagtattag ggacgggaga aaagccagcg
     1081 tggcatttta caccagatgt taccgccgga tttacgccgg aaccttcgcc gtttgaacaa
     1141 atgagtcagg aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc tggttatggt
     1201 ccgcaaaaac cgctgaagct gacgcttttg tataacactt cagaaaacca tcaaaaaatt
     1261 gcgattgctg tagcatcgat gtggaaaaag aaccttggcg tagatgttaa attgcaaaat
     1321 caggaatgga aaacctatat cgatagccgt aacaccggca attttgatgt tatccgcgcc
     1381 tcatgggtgg gggattataa tgaaccctcc actttcctga cattattaac gtcaacgcat
     1441 tcaggaaata tttcacgctt taacaatccg gcatacgaca aagttctggc ccaggcatcg
     1501 acggaaaata ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg
     1561 gagcaagcac cgattgcacc aatttatcaa tataccaatg gacgattaat caaaccgtgg
     1621 ctgaaaggtt atcccattaa taaccctgaa gatgtggcgt acagtcggac tatgtatatt
     1681 gtgaagcatt gatgtgatgg gaactggcgt tacccttgtg gataacgcca gtgatgtctg
     1741 atttagcgac cctgttccgg cggtgtaatg ttatccatat acagcgtctg gctggggaag
     1801 gcaaagtccg cgccgtgtga ctgtacaata tcgataatct tcaaataaac gtcttgctgt
     1861 gcagcaagcc actcagccca taccgtggtt ttggtaaagc aataaaccat aatattcaat
     1921 gacgagtcag caaactggtt gaaataaacc agtaaggttt gtcgctggtc gatggccggg
     1981 tgatttttca gcatctcacg tacggcttcg acaataacgc ccacttttgc cgcatcctca
     2041 taacgtaaac caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg
     2101 ctgatcgacg aaaacagcga gttcggtacg tacaatggac gattatcaaa ggtcgtaatt
     2161 ttggtaattc gccagccaat ttccgctact gtaccttcga tatttctgtc cggtgaacgg
     2221 atccagtcgc caatactgaa aggacggtcg aaatagagca tgatcccgga aaagaaatta
     2281 ctcagaatat ctttaccggc cataccgaca gccagaccac caataccacc aaaggtcagc
     2341 aagccagaaa ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg
     2401 gtgattttga taatgcgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc
     2461 tgctgttttt caaactgatt aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc
     2521 agggacgtac agataaaatc gataacgcct gga
//
LOCUS       DAZUGK010000348         2510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZUGK010000348 DAZUGK010000000
VERSION     DAZUGK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.350"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..191
                     /locus_tag="ACHY5W_004723"
     CDS             <1..191
                     /locus_tag="ACHY5W_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HFS4449455.1"
                     /translation="NLQKKYGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFI
                     ITLPETVRLDVNIHPLKK"
     gene            complement(204..425)
                     /locus_tag="ACHY5W_004724"
     CDS             complement(204..425)
                     /locus_tag="ACHY5W_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449456.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(509..889)
                     /locus_tag="ACHY5W_004725"
     CDS             complement(509..889)
                     /locus_tag="ACHY5W_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449457.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1090..1664)
                     /locus_tag="ACHY5W_004726"
                     /pseudo
     CDS             complement(1090..1664)
                     /locus_tag="ACHY5W_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(1823..1903)
                     /locus_tag="ACHY5W_004727"
                     /pseudo
     CDS             complement(1823..1903)
                     /locus_tag="ACHY5W_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1947..2467
                     /locus_tag="ACHY5W_004728"
                     /pseudo
     CDS             1947..2467
                     /locus_tag="ACHY5W_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          769 a          566 c          451 g          724 t
ORIGIN      
        1 ctaatttaca aaaaaaatac ggcacaacag aaaatagtag ttgggtatcg gcttctgttg
       61 gttctcttct ggacaaagga gccaaagtat atccagatat atcatgctct ctgagactgg
      121 gagaaccatt cattattaca cttccagaaa cagtcaggct ggatgttaat atccacccac
      181 tcaaaaaatg atcctgaacg tttttatgca ggcaaatgcg tccacatcaa ggcttcaacc
      241 ttgtgctctc cccctgtcac aggataaatt tgcctgtaaa aatcaaactc tccctgctca
      301 ttaccaatac caatcagttg aatgcagttg ccatctacaa taaatttttc cgctgacgta
      361 accaaaataa aggcttccgg atgttcatta ggaaaggtgt tagcaagagt acactgaata
      421 tccatctcca acaaaatctc ctgattatta gcgtaaactc atcgacgaaa tcgttactat
      481 cccctgggca catatgtacc attgaatact actgttctcc agattctgat aatccatggc
      541 gaacaataag aacactacag ccgctttgct gattagttag ccataacggt gaactttgct
      601 ggatagtttg agaaatatca tttcgccgga ggcttataca ctgattactc aagcttacaa
      661 ctgcaggtgc atcaaagcgc tccggactat cagtatcacg catagctgta aacagaatat
      721 aatcactacc agccatatca tattccgttt cttttagtct taacaagcca tcctctcttg
      781 taatattaac tgtaattaaa gatgtctcat catgatgggc atagcgctgt gtattaagaa
      841 ttaaatgctc ttccagaaat tttacagggt tactacgtat ttcattcatt atacaccgaa
      901 gctctgaacg atgcggcacc agaaagtgcc cggtattttg tgaaataata ctacttacct
      961 tgattgtttt ctctccaact catgaaaaat aactcgcgta ttatattgtt ttaccaacca
     1021 caggccccga atgcctgtgg tgtttttata taaattatcg gaagttaata ttgtttcatt
     1081 tgaaacatct taactatctt ttataataaa gcttcccctt gcagggtcat atttacactc
     1141 ttctggttta acaatcattg atggggttat tggagctcgt gtcagagggt gaactgaacc
     1201 gtcattaacc acgcgagaaa gtgcatcaac atcaaataaa gtgcatactg ctgaactatc
     1261 tgaatttttg acaaacactc cttcttcggt ctctctagtg taattggaca ctgaatagac
     1321 tctggcgggc actggaaatt tcctgaacta acaggaaact tacattgaga tattttgtcc
     1381 tgaatactct cttgcgattg tgcttcacct gagtccgaaa gcatacttag cattctctca
     1441 ctaagggctc gaggaccatt attaaatccc aaaatcaata actcagaaag caatccgtca
     1501 ttaccgccac tggatgaaaa gcgcccatta gtagcatcat acacaatact gacagtttca
     1561 ccacctactg taatatctcc gcgcccattg gctgcaattc gggcacgaaa aacctccagc
     1621 ccaagattaa atgaacgtga tccaatatct gaggttaatg gcataacaaa actccctatt
     1681 ttaatttgaa ctccagactt aaatagctgt aacaaacatc tgccttatat ggcaacacaa
     1741 aaaccggagc cggactccgt tttttgtgtg tcgggctgtt cattctatcc tttcaacatt
     1801 cagtctgatt ggcatttata ggttataacg tcggtattat cggccactca atatccagtg
     1861 cagttgatgt atcaacacgg ttcagtaaca cccgatactt ttcagtatgc caaagggatg
     1921 atcacccgtg agatagccgc cgcgttcatg ctcatgctaa aacatggggc acacagaaag
     1981 tgatggtatt agatctatat ggcatttcat catcaaatgc tgttgacgga cttacagtcc
     2041 gtgacttact acacaaatat ttaaatgacc aaatgccgga ggtaaagcag accgtactaa
     2101 aagatatgtg ctagaactgc ttatggatag tgacatctcc gcgatcaaac tatctgaact
     2161 gacagaaaat gacgtaattg aacattgcag gctgagaaac aacgctggtg caggtccagc
     2221 aacagtcagt cacgatgtta gttatcttgg cagtgttctg gatgcagcca aacctatata
     2281 cggaattaat tacacatcaa acccggcgaa aagtgctcgt ccatatctac ttaaacttgc
     2341 tttgattggt aaatcaaacc gtcgtaatcg tagaccagca gttgatgaac ttgacatgct
     2401 cattgaagcc cttcaacaac tatctactca taaatgttca aaaattccgt tcgttgatat
     2461 cctcaaaagt tcagcctagg ctatgctgaa ccggttttcc tgaatcattt
//
LOCUS       DAZUGK010000349         2465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZUGK010000349 DAZUGK010000000
VERSION     DAZUGK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.351"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..927
                     /locus_tag="ACHY5W_004729"
     CDS             <1..927
                     /locus_tag="ACHY5W_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFS4449458.1"
                     /translation="SQFPSTLTGPVLFIGMAETAVGLGAGVFDEVRHQHRESVYLTST
                     RHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVLIDDEATTGNTFINL
                     LSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVSGQWGWTPLPDAPVP
                     DMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGERILVLGTGEFVWEPF
                     LLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIPNFVYNVAHQQFDRI
                     LVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            920..1693
                     /locus_tag="ACHY5W_004730"
     CDS             920..1693
                     /locus_tag="ACHY5W_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449459.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            1695..>2465
                     /locus_tag="ACHY5W_004731"
     CDS             1695..>2465
                     /locus_tag="ACHY5W_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP domain-containing
                     protein"
                     /protein_id="HFS4449460.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTP"
BASE COUNT          556 a          660 c          686 g          563 t
ORIGIN      
        1 agccagttcc cctcgacact gacaggaccc gtactgttta tcggtatggc tgaaaccgcc
       61 gttgggcttg gggccggtgt atttgatgaa gtgcgccacc agcatcgtga atctgtctat
      121 ctgacctcta cccgtcaccc ggttgatggc acgttgcttt gcgagttcaa ggaagaacac
      181 agccacgcca ccgatcatct gatctatctg ccagatgatg aagagaaaag acgtcgtgtt
      241 accaacgcac gaacgctggt tttgattgat gacgaggcaa ccaccggtaa tacctttatt
      301 aacctgcttt ctgccctgcg taataccggc aagcttcaac atattgaaca ggttatcgcc
      361 gtaacgctga ccgactggag tggcaatgcc ctgtcggagc gcaccccctt gcccgtcact
      421 tcggtttcgc ttgtaagcgg tcagtgggga tggaccccat tacctgatgc acctgtcccg
      481 gacatgccga aagtcaacgt aacttcacga ggagaatggg acatccaggg gaaacagtca
      541 tggggccgac tggggatgct cgcacctgcg gccgatctcg gccatgaggt ctccgtccac
      601 aagggggaac gtattctggt tctcgggacc ggggaattcg tctgggagcc gttcctgctt
      661 gctgaacggc tcgaagctgc cggagcacag gcattttatg gatcgaccac ccgctcccct
      721 gtcgccgttg gttatgccat tgagtccgcc atttccttta cggataacta cgggctgggc
      781 atccccaatt ttgtctataa cgtcgcccac cagcagtttg accgcattct tgtgtgtact
      841 gagacacccg cagaaagtat tgacacgcag cttcttaaag cgctggctga ggttgcgccc
      901 gtcgtggaga ttgttaccta tgaataaacc cgttgttctg agcgaccttg acgacacgct
      961 ctttcagacc cgtcgtaaaa tggtggacga gctggccctg gagccattcc gtaccggtgc
     1021 cgttgatcgc accctgaatc cacgaagctt tatgacggaa gaacagtcca tgttggtgga
     1081 ctggctcctg gagcaggctg aactcatacc cgttaccgca cggggaactg aagaaatcag
     1141 ccgcgtacgg atccctttcc actcctgggc aatcacaacg cacggggccg tcattctcac
     1201 gcctgaaggc aaacccgacg aggagtggaa ggcccatatg ctcggccagc tggctcccta
     1261 ccaggaaaag ctgacatcga tgcagcgtct gatcactgaa atgatggacg caaaagggat
     1321 caatgcctgg gcaaggctga acttcgaata cggtgaaacg gcggtttata tggtgatgaa
     1381 acaccgcgac agcacccgcc ttgacgagct caatgccatt gcagatgaga tagaaacggt
     1441 gtttccgacc gagggcttct acatccaccg caacagtaat aacgtggcct ggcttcccac
     1501 cccggttgag aaagggctgg cagtcagctg gctgcttgaa aaacttcggg ctgaacgagg
     1561 agttttcccc gtaattggtt tgggcgacag cctgagcgat catcgtttta tgaaactgtg
     1621 cagctggttt ggcatcccgc gtcagagtca gtttgcagat gccatttcac agcgaatttt
     1681 tggagaaaat taaaatgcag ccacatgaaa catttaccgg ctcataccag cccggtgacg
     1741 tggaatttct gctaaagccg gttgtcattg agatgacgcc ggttgagaaa aaagaagaac
     1801 tgattcagtc cgggaagaaa cattattcgg acatgctcag ccaggagcca gcgcctacac
     1861 aatggcatct tgatttgttt caccgggcgc tggatcgggg cgcagagagg cttgcaaaag
     1921 aagtcacaca gctcgctatt gctctggccg aacgcttcgg tgatgagccc attgtactgg
     1981 ccagtcttgt cagggctggc gttccgctcg gcgttatgct gcaccaggcc ctgcgtgaca
     2041 tggggaaaac ctcatggcat tacggtatca gcattatccg ggatcgtgga attgacggtg
     2101 cagccctcga cgtcattgaa gagcggcatg gtaccagcgg tattgtcttt gttgacggat
     2161 ggacaggtaa aggcgcaatt accggagagc ttgtacgcgc actgaaagat cgcccgggct
     2221 atcctgagca gccgcgactg gttgtcctgg ccgatccctg tggctgctcc tggcttgcag
     2281 ccagtgatga tgactggctc attccttttg gcatcatggg ggcgccggta tcaggcctga
     2341 tctcccggtc agtatggtcc tctgaaggat tacacgggtg catggtttgc gagcatctca
     2401 gtgaattcga atgcagccgg atgcttgccg ataccgtcgc tcatttccgt aagaagttaa
     2461 caccg
//
LOCUS       DAZUGK010000350         2462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZUGK010000350 DAZUGK010000000
VERSION     DAZUGK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.352"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            304..447
                     /locus_tag="ACHY5W_004732"
     CDS             304..447
                     /locus_tag="ACHY5W_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFS4449461.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            460..669
                     /gene="cspE"
                     /locus_tag="ACHY5W_004733"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             460..669
                     /gene="cspE"
                     /locus_tag="ACHY5W_004733"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS4449462.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            835..1644
                     /gene="rlmA"
                     /locus_tag="ACHY5W_004734"
     CDS             835..1644
                     /gene="rlmA"
                     /locus_tag="ACHY5W_004734"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFS4449463.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1641..2207)
                     /gene="mntP"
                     /locus_tag="ACHY5W_004735"
     CDS             complement(1641..2207)
                     /gene="mntP"
                     /locus_tag="ACHY5W_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFS4449464.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2339..2429)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          651 a          595 c          599 g          617 t
ORIGIN      
        1 tttattcatt gcatagcaga aaatgtgata ttgcacgcac tatgtaataa cttctcccac
       61 tggcctggaa caactgaact tattgaacta tgttagaaac tacgccagtt taagtatctg
      121 cctgaactgg caaggttaag cacaatgata tatcggcgcg tattccgttg cataagtgtg
      181 caaaaaaagt ggaagacgta tcgagatttg tgcgtctgat cgagacatgt ttaaaaatgg
      241 cttgccataa ttaacgttgt atgtgataac agatttcggg ttaaacgagg tacagttctg
      301 tttatgtgtg gcattttcag taaagaagtc ctgagtaaac acgttgacgt tgaataccgc
      361 ttctctgccg agccttatat tggtgcctca tgcagtaatg tgtcagtttt atctatgtta
      421 tgcctgcggg cgaagaaaac aatctaagga atttttcaaa tggcaaagat taaaggtcag
      481 gttaagtggt tcaacgagtc taaaggtttt ggcttcatta ctcctgctga tggcagcaaa
      541 gacgtgttcg tacacttctc cgctatccag ggtaatggct tcaaaactct ggctgaaggt
      601 cagaacgttg agttcgaaat tcaggacggc cagaaaggtc cggcagctgt taacgtaaca
      661 gctatctgat cgaatccact gatctgaagt gtgaatacgc ttcaatctcg ctataaagcc
      721 tcgtcgaatg cgaggctttt tactatgctt tatcttcgct cctggcgttc ggatatttgc
      781 ccgccgcgtg attcgcgtta cacttgcggc ctttagtatc ctgccggagt tgtcatgtcg
      841 ttttcctgtc cactttgcca tcagcctctt tcgcgtgaaa aaaacagcta tatctgtccc
      901 caacgacatc agtttgatat ggcgaaagaa gggtatgtca atctgctgcc cgttcagcat
      961 aaacggtccc gtgatccggg cgacagcgcg gaaatgatgc aagcacgccg cgcattctta
     1021 gatgccggac attatcagcc gctgcgtgat gcaattgtcg cccaactgcg ggaacggctt
     1081 gatgaaaagg ccacggcggt gctggatatt ggctgtggtg aagggtatta cacacacgca
     1141 tttgccgatg cgttgcccga aatcaccacg tttggtctgg atgtttcgaa ggtagcgata
     1201 aaagcggcgg cgaaacgcta tccgcaggtc actctttgtg tcgcttccag ccaccgtttg
     1261 ccgttttccg ataccagtat ggacgccata atacgtattt acgcgccgtg taaagcagaa
     1321 gaattagcac gagtagtgaa gcccggcggc tgggtcatta ctgccacgcc gggaccgcga
     1381 catttgatgg agctgaaggg gctgatttac aatgaagtac atcttcatgc acctcatgca
     1441 gaacaactgg aaggttttac attacagcag agtgatgagt tgtgttatcc gatgcgtctt
     1501 cgcggtgatg aagccgtcgc attattgcag atgacgccgt ttgcctggcg tgcgaagcca
     1561 gaagtctggc aaacactggc agcaaaagaa gtgttcgact gccagacgga ctttaatatt
     1621 cacctctggc agcgttctta ttaaccgtgg aagtgcgtcc agaggatctg gacgccgatg
     1681 ccgatcagca ccagcccgcc gagaatttcc gcttttttcc caataattga gccgataaag
     1741 cgaccaacca tcatccctaa tgtcgacata atcaaggttg cacaaccaat ggccaatgcg
     1801 gtcgcgataa tgttgacctg aaggaaagca agaccaacac ccacagccat ggcatccagg
     1861 ctggtggcaa tcgcggtggt taccagtagc cagaaaccgt gccgacggcg cggctcttca
     1921 tcttcatcat ctgcgccacg aaaaccctca ataatcattc gcccgccgag gaatatcagc
     1981 agcacaaacg caatccagtg attccattca aggacaaacc ggctggctaa catgcccatt
     2041 ccccagccga tcagcggcgt cagggtttcg acggcaccaa aaataaggcc ggttcgcaat
     2101 gcttcagaaa atttcggttt atggagggtg gcacctttac cgattgatgc agcaaatgca
     2161 tccatcgaca taccaaacgc aagaagaaca gtagcagtga tattcatgac aacgtcctga
     2221 ccgggatatc cataaaacac atcatcgccc ccagcaaaca tgcatacatg aacaacaagc
     2281 cgcgatgggt tttcgcgttc atgcaggcat agcgattgat gtgcatatgg tctcgcctga
     2341 ctgaaagtct tcagtccgta cgtgccacgt tttgcaacga gtatgttgac acgtacattt
     2401 ccggaatctg gaaatcggct actccccaaa tgagggcgca accttaacat attttgagaa
     2461 ta
//
LOCUS       DAZUGK010000351         2451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZUGK010000351 DAZUGK010000000
VERSION     DAZUGK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.353"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..353
                     /gene="yiaW"
                     /locus_tag="ACHY5W_004736"
     CDS             30..353
                     /gene="yiaW"
                     /locus_tag="ACHY5W_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS4449465.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            359..1495
                     /gene="yiaV"
                     /locus_tag="ACHY5W_004737"
     CDS             359..1495
                     /gene="yiaV"
                     /locus_tag="ACHY5W_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4449466.1"
                     /translation="MDLLIILTYVAFAWAMFKSFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMVANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNAQPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1492..>2451)
                     /gene="yiaU"
                     /locus_tag="ACHY5W_004738"
     CDS             complement(1492..>2451)
                     /gene="yiaU"
                     /locus_tag="ACHY5W_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449467.1"
                     /translation="LKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFELKVG
                     LKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGII
                     TYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVAS
                     FITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRD
                     YRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVA
                     IFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          603 a          568 c          625 g          655 t
ORIGIN      
        1 tgttcttttt tatattctct gagggaaata tgttcctgga ctattttgca ctgggagtgc
       61 ttatttttgt atttctggtg atattctatg ggattattat tttacatgat attccctacc
      121 tgattgctaa aaaacgtaat catcctcatg ccgacgccat tcatgttgct ggttgggtga
      181 gtctttttac gctgcatgtt atctggccat ttttgtggat ttgggccacg ctttaccgcc
      241 cggagcgggg atggggaatg caaaaccatg actcatccgt tatgcaactg caacagcgca
      301 ttgccgggct ggagaaacag ctcgccgaca tcaaatcctc ttctgccgag taatacttat
      361 ggatttactg attattttga cctatgtggc ttttgcatgg gcaatgttta agagcttcaa
      421 aattcctgta aataaatgga ccattcccac agcggccctg gggggcatat ttattgtcag
      481 cggtttaatt ctgttaatga actataacca tccgtatacc tttaaagcgc aaaaagcggt
      541 tatttctatt cctgttgtcc cacaggtgac aggcgtggtg atagaagtga cggataagaa
      601 aaatacgctg attaaaaaag gtgaagtgct atttcgactg gacccgacgc gttatcaggc
      661 gcgggtggat cggctgatgg cggatatcgt taccgcagaa cataaacagc gggcgttggg
      721 cgcagagtta gatgagatgg tggcgaatac tcagcaggca aaggccacgc gggataaatt
      781 cgctaaagag tatcagcgtt acgcacgcgg tagccaggcg aaagtaaacc cgttttcaga
      841 acgcgatatc gatgtggcgc ggcaaaatta tctggcgcag gaagcctccg taaagtcatc
      901 ggcagcggaa caaaaacaga tccagagcca gctggatagc ctggtgttgg gtgaacattc
      961 tcaaatcgcc agcctgaaag cgcagctcgc ggaagcaaaa tataaccttg agcagacgat
     1021 agtgcgtgcg ccgagcgatg gttatgtgac tcaggtcctg attcgtccgg gtacctatgc
     1081 cgcgtcgctg ccgctacgtc cggtgatggt gtttataccc gatcagaaac gacaaatcgt
     1141 ggcgcagttc cgtcagaact ccttgctgcg actggctcct ggcgacgatg cggaagtggt
     1201 gtttaatgct cagccaggta aggtattcag cggtaagctg gcagccatta gtccagccgt
     1261 tcccggcgga gcttatcagt cgaccggcac cttacagacg ttaaacacag cgccgggttc
     1321 agatggcgtt atcgcgacca ttgaactgga tgagcacact gatttgagcg ctttaccaga
     1381 cggtatttac gcccaggtgg cggtttactc tgatcatttc agccatgtct cggtgatgcg
     1441 caaagtgctg ttacgcatga ccagttgggt gcattacctt tatctcgatc attaatcttc
     1501 gctttccgcc gcttgttcag gccgcagttg ctgcgactga gcaagctggt ggcgaatttc
     1561 acgcagcatt tcatccagca catactggac ccgccagggc tggtattccc gtttacggaa
     1621 gatagccacc agatccagcc accattcata cgggtgctga aagcagggca ccagcgtgcc
     1681 gtcttgcagg tcgctctgca cacttttgtc tggcgcaaac acaatcccca ggtgatttcg
     1741 tgccagctcc agcgcagatt gcgtgttgtc gcaaacgtaa tttcccttca cgcgataatc
     1801 ccgcacctct ttactcccgg cgacgttgaa gcgccagata ttcgcgtcgt cgatcatcat
     1861 cgagtcgatc aaaatacagg agtgatgttc gagttcgtca ggacggctaa tgggatgttt
     1921 ctcaagatag cgctggctgg cataagcggt tacggcatat tgggtaataa aactggcaac
     1981 cagcgattca tcttttggtg gagcgtagct gattaaaaca tcacaatcat caggaaattc
     2041 gacgccttca taaaaggcat tacgctcaag attgcaggtt tttagcgata aggtgatatc
     2101 accgatatct ttaattttat cgattacatg tttggataaa taggtaatta tgccggttgg
     2161 ggcgtagatg gtgacccgac cacgtttctc atgcttataa tctgcaataa aattattaag
     2221 ctgctcgttt ctgtccagca tgtcgttgat gtacggtaac agcgcggtac caaaaggtgt
     2281 gagcatcagc tgccgggtgg ttcggtcaaa aacttttaaa cccactttta attcaaaatc
     2341 agcaagatat ttgctgacat tggcctgtgc gatgccaagt acagtcgcgg catggctgat
     2401 attttcactg gcagcgatta ccgagataat ttttaactcc cggtacttaa g
//
LOCUS       DAZUGK010000352         2446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZUGK010000352 DAZUGK010000000
VERSION     DAZUGK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.354"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(124..1662)
                     /gene="cadC"
                     /locus_tag="ACHY5W_004739"
     CDS             complement(124..1662)
                     /gene="cadC"
                     /locus_tag="ACHY5W_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFS4449468.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1922..2081)
                     /locus_tag="ACHY5W_004740"
                     /pseudo
     CDS             complement(1922..2081)
                     /locus_tag="ACHY5W_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          705 a          484 c          526 g          731 t
ORIGIN      
        1 tctggttaaa tttatgtaat aaaaattatg cggcaaataa attgccgcaa catattatac
       61 caacaggaac atacaaaaac tcaacaacaa atattcccga gcatgaatca acccgggggt
      121 tacttattct gaagcgagaa atttgtcgag ataaggtaca acataaggaa cagaagtctg
      181 gaatatacca ttttcaatcc agtaaagggt gtttgcccct gggcgtaaat taaaggcggt
      241 gagatatgca tcagccgctt cccggttcat ccccttcatt tcataaacct tgccaagcaa
      301 tacataattt agccaggaca tttcaagatc aatgccagta tttatcgcct ggtaagactc
      361 atctgtttta ccttttacca gagcactgac cgcttttatt tgatatataa tggacaggtt
      421 attcaattcc ggtagtgtaa caatgttatc tatttctgtg ttcagtgctg ctaattgttt
      481 ttcatctaaa ggatgttgag aatggcgcac gatatcaact aatgcttttt ctgctctcgc
      541 gtaggtaaat tctggggatg attgaacaat ctcacctaat aattcactgg cacggttcaa
      601 tgatttatca tcgccatgca gtaaataatc atgtgcctga taaaaattag ttaataacgc
      661 accacgatga ggcaaaattt gctggagtgt ctcctgcatt cgttgtggcc acggttggtt
      721 taacgctttt gataaactct ccagtaaatc attttgaatc gccagctgat taccgttagt
      781 gatgacataa cgtttatcca gcatggttga accatctgca ttgtctacca attttatcga
      841 cataaagcat tgttgagcac ggtattggcg ctgattaaca aacgcaatag ataatgtttt
      901 accggaactg ctcggttcat caatgttgta gttgattttg tcatgcacca taaaggtgga
      961 gaaggtgtta agtgatgtcg ccaccaaatc acccacgcct atcgcgtaag agagctgata
     1021 cggggaactc cagctgttac aacttttatt taccatatta atgtcaatat cgcgtggatt
     1081 gagcaaaata cgcgatttgc tcatcggaag acgtgtatca agacttgaaa acgctaccag
     1141 tgctacacag atacctaagg acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg
     1201 tttgctttta actggggatt gttcaggtgg cgttgcggtg ttttgaatgt taagactgtg
     1261 ggagggagaa tctgtggcag gaaccgcctc tggtataggg ggaggcgaag atagcattat
     1321 ttcctctccc tcttcttcgc tgtaccagat aaccggcacc attaatttat agccgcgctt
     1381 tggtacagta gcgatataga caggactatc ttcatcatta tcttttaatg acttacgtag
     1441 ttctgagata ctctgcgtca caacgtgatt ggtgacaata cttctcttcc agacattatc
     1501 gataagttca tccctgctaa gtacttcgcc actgtgttga gcaaagaaaa ccagaaggtc
     1561 gattaatctc ggctcaaggg taagttgacg cccattgcgg ctaatttggt ttatggacgg
     1621 agtaacaagc cattcgccaa cgcgaactac aggttgttgc ataatagaaa ctcattcgaa
     1681 aagggaatga tgcaatgata attgccacaa cctattttta ccatctatag atgggtttat
     1741 ttacatatta ttggtgaatg caagacgtta tttttaccag ccataaactt cttgattaca
     1801 tggtattacg aaaggatttt actgagaacc agaagtaata ttccttacca tcaaaattca
     1861 tcatctttgc caaagaaaaa tgttcagaaa ataatccatg gaaaattgtc cggagcgctt
     1921 actattttaa tggattgtta gtctttgcat gagcaagcgg actgatacat ttctctttgt
     1981 tctcattcag aaaatctcat cagtcgccgt tcagaccggt gttgtgcttt atggatgcgt
     2041 tacgctcctg atgacgtcat ttgacgttca acagcatcac ggggtcgcac gacatttcac
     2101 gtcagttagt gctatcgctc aggaacaaat tttcccgaat tgggatatgc ccgcaaattg
     2161 ctggtgatgt ggggagaatc tggttgagtt cggtagaatt gatttggagg cagaacgctt
     2221 aaatcgtggc gtcctgaaac gaaaaacgga cctccgtgga ggtccgttta tatgaatttg
     2281 gtgccggact cggaatcgaa gaatgcttct atcatgttga tattagttta ttttgtttgg
     2341 tttggtgaaa tatgtaccct cttttgtacc cgcagtccct tttgatatcg aaagggaatc
     2401 aaactcgggg tcaggctgag agcacagaag gaaagtgcct ggctat
//
LOCUS       DAZUGK010000353         2429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZUGK010000353 DAZUGK010000000
VERSION     DAZUGK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.355"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(163..1575)
                     /gene="pykF"
                     /locus_tag="ACHY5W_004741"
     CDS             complement(163..1575)
                     /gene="pykF"
                     /locus_tag="ACHY5W_004741"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFS4449469.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1583..1777)
                     /locus_tag="ACHY5W_004742"
     CDS             complement(1583..1777)
                     /locus_tag="ACHY5W_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449470.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILKARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2132..2341
                     /gene="fumD"
                     /locus_tag="ACHY5W_004743"
     CDS             2132..2341
                     /gene="fumD"
                     /locus_tag="ACHY5W_004743"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFS4449471.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          645 a          521 c          589 g          674 t
ORIGIN      
        1 aaaattgaga gaaaacagga tgcttccatc ggattcatct tagataaagt gagattatat
       61 agtgaaatcc gatttgattt tttattgctt ctggttatcg attaaataaa aaaagcgccc
      121 atcagggcgc ttcgatatac aaattaattc acaaaagcaa tattacagga cgtgaacaga
      181 tgcggtgtta gtagtgccgc tcggtaccag tgcaccagaa accataacta caacgtcacc
      241 tttgtgtgcc agaccgctct gcagagccag ttctttaccc agacggtaga aatcatcagt
      301 agaagtgatc tctttaacca gctgcggcac aacgcctttg ctcagtacca actgatgagc
      361 cgttttttcg ttggtggtca gtgccaggat ggtggcatcc gggaagtatt tacgtactgc
      421 gcgagcagat ttaccgccct gggtagcaac cacgatcagc ggagcatcta gtttttcagc
      481 agtttcaacg gcaccacggc ataccgcttc ggtaatgcgc agtttacggt tgtcattgtt
      541 gaactcgaga cggctgttca tcacgcggtc ggtacgttcg cagatggtcg ccatgataga
      601 aaccgcttcc agcgggtatt taccttttgc ggattcacca gacagcatca ctgcgtcagt
      661 accgtcgagg atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt
      721 gatcatggaa tccagcatct gggtcgcagt gataacgact ttacgtgcac ggatacattt
      781 ttcgatcatc atcttctggg cgaagataac ttcttctacc gggatttcta cacccaggtc
      841 gccacgcgca accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc
      901 ttcctggttt tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg
      961 ctcacggatt tcgataacgt cagaacgctt acgaataaag gaagcagcga caaagtctac
     1021 gccttgttcg caaccaaaga tcaggtcctg tttgtctttt tcagccagtg ctggcagggc
     1081 aatggaaacg ccaggcaggt tcacaccttt gttttcaccc aggtcgccgt tgttcagcac
     1141 tttacagata actttgttac cttcaatggc agtaacttcc ataccgatca gaccatcgtc
     1201 aaccagtacg gtgttgccaa cagacaggtc agttgtgaaa ccttcatacg ttaccgcaac
     1261 catttcgctg ttgccgataa cagatttatc agtagtgaaa gtaaaggtct gaccagcttt
     1321 cagagaaacg tcgttaccgc cttccagttt catggtgcgg atttccggac ctttggtatc
     1381 aagcaggata gcggcggttt taccagtttt gctcatcacg ttgcgcagat tctgaatgcg
     1441 ctgaccgtgt tctgcatagt caccatgaga gaagttcaga cgcataacgt tcatgccagc
     1501 gtccagcatt ttagctaaca tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat
     1561 tttggtcttt ttcatgacag tcttagtctt taagttgaga aggatgggag aaacttgctt
     1621 tctgggcgca ctgccggaaa gcgaatccgg tattacgaaa gtggtggtgc ctcgctttaa
     1681 ggataggtga catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg
     1741 agtgcctgtt tttgagtcgt tgagttctac agtccaaagt gctgaaacca ttcaagagtc
     1801 aattggtgcg cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa
     1861 aaatatacaa cttgttagac aataaattgt tatcaaagat ttaacgttcc agggaaaagg
     1921 ttacgcaaaa acttcgctgt ttgcgcaacg tctggtgccg gggttgtcac tgtgaagata
     1981 agtgaaagtt catccggtgt ataaatacat catcgctcaa ttgctacagt taaaataatt
     2041 tatcgcgtta tttgtctgat tttgtttgat ttttgtttaa ttggttagat tgatatatgc
     2101 tgatagttgg catgataaat aggtataaag aatgggaaat cggacaaaag aggatgaact
     2161 gtaccgagaa atgtgcagag ttgtcggtaa agttgtgctg gaaatgcgcg atctggggca
     2221 ggaaccaaag catattgtta tcgcaggcgt attacgtaca gcattagcca ataaacgcat
     2281 tcagcgcagt gagctggaaa agcaggcgat ggaaaccgtc attaacgcac tggtaaaatg
     2341 accattcagg atgaggcgat aagcttcatc ctgatattct tgcttcagtt gcggtccagt
     2401 taatcattct aaataacaac tgctgaaat
//
LOCUS       DAZUGK010000354         2429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZUGK010000354 DAZUGK010000000
VERSION     DAZUGK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.356"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..592
                     /gene="espM1"
                     /locus_tag="ACHY5W_004744"
     CDS             2..592
                     /gene="espM1"
                     /locus_tag="ACHY5W_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFS4449472.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(811..1867)
                     /locus_tag="ACHY5W_004745"
                     /pseudo
     CDS             complement(811..1867)
                     /locus_tag="ACHY5W_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2099..>2429
                     /locus_tag="ACHY5W_004746"
     CDS             2099..>2429
                     /locus_tag="ACHY5W_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFS4449473.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDN"
BASE COUNT          668 a          468 c          570 g          723 t
ORIGIN      
        1 tatgccagta aatgcgacag gtgtgtcctt cagctctttt ggtatcagtt atcataaaga
       61 taattctttc cggggcacca tccgtgggaa gaatgatgag gtcgtgaagt gttcaatggg
      121 agagcgctct atccgcttta atgttaacaa atttagcggc tgtatactgg agacggtaag
      181 caggcagagt actaaagata ttcatggatg ggtaagtgat gaacggacag tatatccgtc
      241 aagggttatc aaccaagaga ttgataattg ctgccttcaa aaaaatgcaa aaatttcttc
      301 tgaagagagg aaaatggtct tttctcttgt aagtaaggag tttgaactaa ctcttgatgt
      361 taaagcggca caaagttcta ttaatcatat aataatagga aatgcttctt ttggcaaaaa
      421 aatggatgct ctttgcgatg gtatgagccg agctgtaaaa aacagtacaa cagattacat
      481 agcaaacgtg cttgcagaca agttttatca gaaacatatc gccccgggtg ttgatattgt
      541 aaaactacga aatgaaatcc caggttatat gagtcgtgtt atacaggggt aaggttggag
      601 gaggctatgc gccagtatca ttggttttca tcctgtaaaa agatatgagt aaaatataca
      661 acaggcgtta cactatatgt atgtatccaa ataactcata gttcacctgt acagttataa
      721 atcgtaaaga aaattgcagc gcgtcgtacg cagaaacgtg ctgtgattga ctatttatag
      781 ctttgtcaca acgacaagat tcagtagtgt ttacacgggc gggagaaatg acggaataac
      841 taaatagctg cgcctaatat cgcacatttt tgccagccca tgtttgcctt cggggaatgg
      901 actgacggat tccataaaat ggcgaacttt ttcaatagtt gccacattga gcggcactga
      961 tgattacgcg ttattgtggc gtgaagtatc tgccatagct attcaaaatg attcacccat
     1021 ggagattaaa ccggctgata aatgactctg cacttcggtt tatccttcag ccagcgctgg
     1081 gtttcccggc ctttgtggat aatgtagttg tcctcgagca gcgtgatggt tttcgtccga
     1141 cagtatgtag ctttaagccg cttcagcagt ctgatgaaca gcgccgaact tttgctgttg
     1201 gcgcccacac agctgactta acctgtcccg ctgtgcagcg ctacgtccag agaatatttt
     1261 tcattttgtt ccggcgtgac ccctcgtttt tgcttcccga gcagtttcca gtccgcaccg
     1321 attttgggat taagatggat aaatacttca tcttcataaa agaccggatg ctctgtgctg
     1381 cattcgtcca gtgcgttatg gattgttgcc atctttttat ctttaggcgg ggcacggata
     1441 cgtagagttg gcgcagccct tcgccacaca agccccacag acgacaacca gcggcgaatg
     1501 gttccagcat gtaacaggca gccggttatc tcattgattt ttattgtcag cagttctgta
     1561 ctccagcgtg aataattgta gccaaagtcg cgagtgaata ttttacctgt tcacgtaata
     1621 gtgtgtagat atgcgcaaac ggccagcggc gggctcgctc ggcaggtaat gatttcagtc
     1681 cctcaacacc cgactgtgtg aactagttaa tccagcgccc aacagaggaa cgggcgcagc
     1741 atagtgtgcg ggtaacgtcg ctgacacggt cgccccggtg cagcatcagc atggctgtca
     1801 gccttttatg gatggctttc tgtattaggc gtcgttcgtc acgggaaatt ggtggtatga
     1861 tcgacattgc tcagtccggt tggtgatttg tattgatttg gcgattgatc agatcgcaca
     1921 atcgggttga gttccctcta aatgatctac tattctgcgc agctatttag tgagtatata
     1981 ttttatgctt ataaattaga ttaaaataat cctacaatta ttctgatgga gtattgctgt
     2041 ggaggatggc tttttgttaa ttatcgaaaa gtaagatata tgctttaggg ataaaattat
     2101 gccattttca tttaatttgt cttctggaaa ttacttgtca acacaagatg ttgaagtttt
     2161 acaacgtgcg accagagatc atcaaatgga aagactcaca ataggagaaa gatcgttttc
     2221 tgttcgctat cagagtgcta tggacgcttt tattgtagat cctgttcaag gggaactata
     2281 ttcgggttta agccatacag aactagccga tatcattaga ttggctgatt ctgttgaaaa
     2341 tcaattgaat ggaggcaatt catttcttga tgtattcagt acatatatgg ggcaggttat
     2401 ttctgaattt atgcatagta atgataaca
//
LOCUS       DAZUGK010000355         2408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZUGK010000355 DAZUGK010000000
VERSION     DAZUGK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.357"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..432
                     /locus_tag="ACHY5W_004747"
     CDS             148..432
                     /locus_tag="ACHY5W_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFS4449474.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNVG"
     gene            451..1923
                     /gene="tssI"
                     /locus_tag="ACHY5W_004748"
                     /pseudo
     CDS             451..1923
                     /gene="tssI"
                     /locus_tag="ACHY5W_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
     gene            2046..>2408
                     /locus_tag="ACHY5W_004749"
     CDS             2046..>2408
                     /locus_tag="ACHY5W_004749"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFS4449475.1"
                     /translation="MKQLTLTGCGAACGEMLLRDRYIFVTQNVIGTELTSMTSLANKL
                     NKFDVGWEGNAVSESSLYALSNTGSWGAMIWDSGSKVGHWVLVKGVDDAGNVIIYDPY
                     QGSRYLMTEQEFKEVWNGH"
BASE COUNT          582 a          523 c          768 g          535 t
ORIGIN      
        1 aacttaattc acgccaacaa tttgtattac ttaaatagac cagtgaaggt acgtatcaga
       61 atatccggtg aagtgattaa tatttcgttg aatatttctc cggatttttg aaacctgctg
      121 ctgtagaaat tctgaacagg aggaattatg cctttagcag ccaaactcac cgacaaaggc
      181 acccagcacg acggttatta cgagaccgtc atcaccgccg ggtcatccac cgtttttatc
      241 gatggtctcc ccgcagcccg ccagggagat ccgctgacgc cgcatgctaa accgaagcat
      301 ccccctcatc cccgtaaaat cgccagaggc tcgtcaaccg tctttattga cggcctgccc
      361 gccgccagaa ccggtgatgc catcgactgt ggtggtgtgg tcattggtgg cggtacggta
      421 aatgtaggct gatatgtctg agaggtattt atgtccttaa aaggtctgcg ctttacgctg
      481 gagattgacg gccaggagcc ggacaccttt gcagtggcga atttcaggtt aatccagaac
      541 cagtcatatc cgtttgtgat gagtgtggat gttgccagcg actcttttat gcagacggcg
      601 gggatgctgc tggagaagaa ggcgacgctg accatctggc agggcgtcat cccgctgcgt
      661 tatgttaccg gcgtggtggc gggctttggc atgcaggaga acaatggctg gcagatgcgt
      721 tatcacctgc gtattgagcc gccgttatgg cggtgcgggc tgcggcagaa cttccgtatc
      781 ttccagcagc aggatatccg gacggtatcg gccacgttac tgaacgagaa cggcgtcacg
      841 gagtggactc cgctgtttta tgaagaccat ccggcgcggg agttctgtgt ccagtacggt
      901 gagagcgacc tggcgtttct ggcgcggctg tgggcggagg aggggatttt cttctttgag
      961 cggtttgcgg cggacagtcc ggagcagaag ctgacgctgt gcgacgatgt ggcggggctg
     1021 tcacaggcgg gggagtttcc gtttaacccg gacacatcgg caggagcgga gacagagtgt
     1081 gtcagtatgt tccgttatga ggcgtatgtc cgcccgtcgt cggtacagag ccaggattac
     1141 acgtttaagg tgccggactg gccggggatg tatgagcagc agggtgagaa cctgaacgga
     1201 cagctggcgc agtatgagat atttgattat ccgggaaggt tcaaggacga gcagcacggc
     1261 aaggatttca cgctgtacca gatggacagc ctgcgcagtg atgcggaaaa ggccacaggg
     1321 cagagtaatt cgccgaggct gtggccggga acgcggttta cgctgacgag gcatccgcag
     1381 aagatgctga accgtgaatg gcaggtggta cagagcatac tgtcaggaag ccagccgcag
     1441 gcgctgcacg gcagccaggg aaagggaacc acgctgggaa atcagttaga ggtgataccg
     1501 gcggaccgga cgtggcgtcc acggctgcaa aacaaaccga aggtggacgg gccacagagc
     1561 gccattgtca ccgggcctgc gggagaagag attttctgcg acgagcatgg ccgggtacgg
     1621 gtgaagttcc actgggaccg ttatcacggg atgacggagg cgagttcatg ctgggtgcgg
     1681 gtgtcgcagg cgtgggcggg gccggggttc ggtaacctgg cgataccgcg ggtgggccag
     1741 gaggtgattg ttgattttct gaacggcgac ccggaccagc cgataatcat ggggcggacc
     1801 taccatgagg acaaccgttc gccgggcgac ctgccgggaa cgaagacgca gatgaccatc
     1861 cggtcaaaaa cctataaagg aagcggattt aacgagctgc ggtttgaaga cgccaccagt
     1921 aatggatacc cgcaaaaata ccagaagaaa atatctctgg gaaagtattt gtagagccgg
     1981 tagttttaga aggtggtgct ggtggcaatt ggacagtatt tgatgaggtt ttagattcaa
     2041 atgtaataaa gcaattgact ttaacaggct gtggagcggc ctgtggggaa atgttgctta
     2101 gggataggta tatttttgtc acgcaaaatg taattggaac agaattaaca tctatgacgt
     2161 cgctggcaaa caagcttaat aaatttgatg tcggctggga agggaatgca gtgagcgaat
     2221 ctagtttata tgccctcagt aatactggtt catggggagc aatgatatgg gattctggaa
     2281 gtaaagtagg gcattgggta cttgttaagg gtgttgatga tgctgggaat gtgataatat
     2341 atgatccata tcaagggagt cgatatctga tgactgaaca ggaatttaag gaggtatgga
     2401 atgggcat
//
LOCUS       DAZUGK010000356         2405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZUGK010000356 DAZUGK010000000
VERSION     DAZUGK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.358"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..446)
                     /locus_tag="ACHY5W_004750"
     CDS             complement(<1..446)
                     /locus_tag="ACHY5W_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449476.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTAS"
     gene            complement(446..787)
                     /locus_tag="ACHY5W_004751"
     CDS             complement(446..787)
                     /locus_tag="ACHY5W_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449477.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(780..>2405)
                     /locus_tag="ACHY5W_004752"
     CDS             complement(780..>2405)
                     /locus_tag="ACHY5W_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4449478.1"
                     /translation="VEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKVYQS
                     MLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALS
                     RLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGKSET
                     EKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKTLTEMQKQELLRNATLID
                     QQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDSFRQKDA
                     DLQSQYQSGDISEDFYRQALAQNAQYLSERLKDQETFYAESDAQRADWQKGLQEGFSN
                     WVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLMNAAI
                     VNGIKTAANSMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTLTYFAF
                     AKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQ
                     IGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          455 a          833 c          538 g          579 t
ORIGIN      
        1 gaggctgtca tggccgtcag ctctgacatc tgctccacca cccagcggtc tctcagctcc
       61 tgctccgggt ccgcttccgg attgcccgcc acaaaattca ccgcatccag aaaacgggca
      121 tacacccggc ggcggaccac cgtggccccc accaggctct gcaggtcctc cgccattccg
      181 gtgaccagac cgaacagatt ggacaccgtc agtgacgggc gggcactgct gcccttcccg
      241 ttcatctcaa aaccgctgcc gtcaatcggg tatgcctgat attcccgccc ctgccaggta
      301 accgcctccc ctttttcatt cagctcattg cagaaaaaat accgctcacc accctgtacc
      361 gtcaggtcga tttcccagag taccacccgc ggtgactgct ctgacttaac cgactcgttc
      421 agactttctt cgcgaatatc ctgcatcagt tcaccacctg ctcaatcgta caactgaaat
      481 cactgtacct ggcgttatct gtgacgctcc actcccggca caccaccctc accgtccggt
      541 tatgtttcgg cggtcgccac aaaaaggcac ggtaaccacc atgccaggat aaaaattcat
      601 ccagccagcg ccgggttggt tcatccgtca cccggaacac cgcctgaaac gtcttcagtc
      661 tggcattaag tcccgtcggt cggcgctgtt cataaccgtc accaaaccgc accctcacca
      721 ccgacggctt ctcactcacc tgcatccctt cacgcgggac cagatgcagc gtttttatct
      781 cagccactca gcattcctcc gtcacgtcgc atggacagca tcaccgcctg cacccgctgg
      841 tcaatcagct gcacaaggct gcccgcagct tccggcccta tctgtccgtt agccccgtca
      901 ttctgaatgg cgatgtggta gaccggggaa tacaccagac cagcactgcc gttcatactg
      961 cccaccgcgc gtacgcccag cgagccatcc gccgcccggg tcaggggcat aatggcttca
     1021 ggtccggctt cccccatcag cccggcccct tttgcaaacg caaagtacgt gagcgtgtcc
     1081 acaatgctgt tgctgtacgc gctcaggttt gccgaggtat acacgccgcc ttttgcattg
     1141 gccaccgcac cgcccagcca gtcaccaatg ctgccgagaa atcctcctgc accggacata
     1201 ctgtttgccg ccgtcttgat cccgttgaca atcgcggcat tcataagaac ttttgatatt
     1261 tcctgcagca ctgatgaggc ccagctgcgc cattccactt tatttccgtt cagcatctcc
     1321 gtgatgttat tcaccatccc tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg
     1381 taatcggacg cattatccac ccagttactg aatccctcct gcagcccttt ctgccagtcc
     1441 gcacgctgcg catccgattc ggcataaaaa gtttcctggt ctttaaggcg ttcactcaga
     1501 tactgcgcgt tctgtgccag agcctgtctg taaaaatcct cactgatatc cccgctctga
     1561 tactgagact gaaggtccgc atccttctgg cggaagctgt cgcggatctg ctgcaactcc
     1621 cgcatgcgtt ctctggctcg ctccccctgc ccgtacccca gcagttcagc atcattcgac
     1681 gcacgcgcag ccgcattctc attcttcagt gtctcttccc gggatcgcaa ctgttcccgg
     1741 attttttgct ggtcaatcag ggtcgcgtta cgcagcagtt cctgcttctg catctccgtc
     1801 agggttttca gttcacccag cgctgtctgg tatttcagct tcgccagctc tgtattctgc
     1861 cccaccagtg ccagttgctc tttctgctgc ttcagcagcc gggaaaaact gtcttccgct
     1921 ttttccgtct ctgattttcc accccgggat ttgggtttat tcgcctcgtt attgcgccag
     1981 gcttccaggg cattactgat ataacgttgt ctcgcctcct gatacggatc acccacaaaa
     2041 ccgaggtcat ccgccgcata ccccagtcgg acacgctctt tttcttcccc tttcagtctg
     2101 gacagggcca gctcacgctc tgtttttgtc agggcactct gctgtttatc atccagggtg
     2161 gcctgcggca gccgtaacgg cacattcacc agtccctgcc gctgctgaag cagttcatta
     2221 cccagcccca gcagacggtt gaattccgta tgctgaccgt tcataaccag catggactgg
     2281 tacaccttat tctgctctgc cgcctgctga cgaattaacg ccacacgacg gtcttccagc
     2341 ccggcaagca catcctgaat ggactgcgct ttttcctgca tctgtgccag acgggactgc
     2401 tcaac
//
LOCUS       DAZUGK010000357         2395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZUGK010000357 DAZUGK010000000
VERSION     DAZUGK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.359"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..827
                     /locus_tag="ACHY5W_004753"
     CDS             3..827
                     /locus_tag="ACHY5W_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS4449479.1"
                     /translation="MPYSLSRHILEHLRKLTSHEPVPGIMGKSGAGKSSLCNALFQGE
                     VTPVSDVHAGTRKVRCFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLV
                     LWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAR
                     NIREKTEAVFRLFRPAHPVVAVSARTGWELDTLVSALMTALPDHASSPLMTRLQDELR
                     TESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            1045..>2395
                     /locus_tag="ACHY5W_004754"
     CDS             1045..>2395
                     /locus_tag="ACHY5W_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFS4449480.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     "
BASE COUNT          591 a          628 c          657 g          519 t
ORIGIN      
        1 ccctgcctta ctcgctttcc cgtcacatcc tggaacatct gcgcaaactc accagtcacg
       61 aacccgtgcc tggcattatg gggaaaagcg gggccggtaa atcctcactc tgtaatgccc
      121 tgtttcaggg agaggtcaca ccggtcagtg atgttcacgc cggcacccgg aaagtgcggt
      181 gcttccgtct gagtggccac ggtcacagca tggttatcac tgacctgccc ggggtgggcg
      241 agagtcggga cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaactgg
      301 acctggtact gtggctgatt aaagccgatg accgtgccct gtctgtggat gagtatttct
      361 ggcgacacat cctgcaccgg ggacatcagc aggtgctgtt tgtggtgacg caggccgaca
      421 aaacggagcc ctgccatgaa tgggatatgg ccggcattca gccctctccc gcacaggcac
      481 ggaacattcg cgaaaaaacg gaggcggtat tccgtctgtt ccggcccgca catccggttg
      541 tggccgtatc ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag
      601 cacttcccga ccatgcctcc agccccctga tgacccgact gcaggacgag ctgcgcacgg
      661 agtctgtccg ctcgcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca
      721 cagcggagag cgtctgtgtt gcctctgttg tccgtacggc cctgcgcgct gttcgtgaca
      781 ccgtggtctc tgttgcccgc gcggtatgga actggatttt cttctgaacc cggtggtgat
      841 gcaccagact cttgttacag atttttaacc ccatgaaaac acaatgcaaa cacagccata
      901 cccgggttgt ccccttgcgt tgtgtttctc aagttaatag aatgcatcga tcgttactat
      961 tatatcgatc gttataaacg atcgtttgtg gtaaatgacc tcctccgtgg ttgtcatgaa
     1021 aatgcgtctc aggaaggtta aataatgcac gaaaagaaca tcgccctgct ttgtgatgaa
     1081 gccgaccgac ttttgcaact gaacattaat ctgctccggc aaatggttga tgaaccggat
     1141 gtgttatctg acagtaagaa cgaaaacaga ctgctttttg ataaacagaa agcactgaaa
     1201 agaattgagg agctggaggg cgaacaaatc aaaaccgccc gcagggagat ggtgctggct
     1261 gttgtcggca cgatgaaagc aggtaaatca actaccatca acgccattgt ggggcaggaa
     1321 attctgccta accgtaaccg ccccatgacc tctgtaccga cgctcatccg ccacgttccc
     1381 ggaaaaactg agccggtcct ccatctggaa catattcagc ccgtccgcaa tttattaagc
     1441 acactgcaac aaaaactcga caccccggca ggacagcaag tcgcacagac cctgcagcaa
     1501 accggggata cctgcgaact gctggatatt ctggcaaatg atggctggct caaaaatgaa
     1561 taccacgggg aggaggaaat ctttaccgga ctggcatcgt taaacgatct ggttcgcctt
     1621 gctgcggcaa tggggagtga gtttcctttt gatgaatacg cagaagtgca gaaactgccg
     1681 gtgatcgacg tggaattcag ccatctggtg gggatggatg catgccaggg aacactcaca
     1741 ctgctggata cccccggccc taatgaggcc ggacaaccgc agatggaagt gatgatgcgg
     1801 gatcaactgc agaaagcctc tgcggtgctg gcggtgatgg attacaccca gatgaactca
     1861 aaagcggatg aagacgtccg taaagagctt aatgccattg ctgacgtatc agccggccgc
     1921 ctgtttgtgc tggtcaataa atttgatgag aaagaccgca atggcgatgg ggcagatgcc
     1981 gtacgccaga aagttccggc aatgctgaac agtgatgtgc tgcccgcctc ccgcgtttat
     2041 ccaggctcat cacgtcaggc atacctggct aaccgtgcgc ttcatgagtt acggaaaaac
     2101 ggaacccttc ctgttgatga agcctgggtc gatgattttg tcagggaagc cttcggtcgc
     2161 atgaaaaaag aatacgtctg taaagacagt gaaatggcaa ccgaaggggc gacagacctg
     2221 tgggaaggtt cacttatcga tcaactgata acggaagtca tacagagctc ccattccaga
     2281 gcggcggcac tggcggttga ttctgccgcc gcaaaactga tgcagaatgc ggaaaatgtc
     2341 agcgaatatc tgtcattacg ccatcagggg ctacagcaaa gcattcagtc actgc
//
LOCUS       DAZUGK010000358         2391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZUGK010000358 DAZUGK010000000
VERSION     DAZUGK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.360"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            111..614
                     /locus_tag="ACHY5W_004755"
     CDS             111..614
                     /locus_tag="ACHY5W_004755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4449481.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(660..1076)
                     /gene="rraB"
                     /locus_tag="ACHY5W_004756"
     CDS             complement(660..1076)
                     /gene="rraB"
                     /locus_tag="ACHY5W_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFS4449482.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1238..2242
                     /gene="argF"
                     /locus_tag="ACHY5W_004757"
     CDS             1238..2242
                     /gene="argF"
                     /locus_tag="ACHY5W_004757"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4449483.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
BASE COUNT          615 a          554 c          590 g          632 t
ORIGIN      
        1 atcagtaata ggctggcaaa aactttattg ctttataaga tgcttgcgca cgtaagaaga
       61 gagtggtatt aaacaaatgt ataattactt tatttttatt gggttaaact atgaataaca
      121 ttgcgccgca atcacctgta atgcgtcgcc tgacgctgca ggataatcct gctattgccc
      181 gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg
      241 ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca gcctggtcat gcatattggg
      301 tggttgagta cgagggtgaa gtggtcggcg gcggtgggat tgcgccatta gcaggtagtg
      361 aatcggacat ttgcgaactg caaaagatgt attttcttcc ggctattcgc ggcaaagggc
      421 tggcaaaaaa actggcctta aaggcgatgg aggaggcaag agaaatgggc ttcaaacgct
      481 gctatctgga aacgaccgct tttttgaaag aagcgattgg tttgtatgaa catttaggtt
      541 ttcaacatat cgactatgca cttggctgca cgggccatgt cgattgtgaa gtgcggatgc
      601 tacgcgaact ctaaaaaaat gcccgctagt gtgtgctatc gggcatttta atcaattaat
      661 tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt catcgtcgcc gtcttcgccg
      721 ttgggatctt caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc
      781 gtcatcagtt gttcaacctg ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg
      841 atgtcgcagc aaatcacgat atcaccatct tcaacttcca gctcttctgg atcggtcact
      901 tcgtaaccga gtttaaacgc ttcaactgct gctttttcca gggtttccag atcgtctgcg
      961 gaaagatggt gttcgatggt gtacagcgcg tccggatcgc tgccatcttc cagtaattct
     1021 tcaataatca aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt tgccatggct
     1081 cattcctctt aatgtcctgc cgatactcat attgtcacac accactggca ttgcctccac
     1141 ctttgtgaca aagatttatg ctttagactt gcaaatgaat aatcatccat ataaattgaa
     1201 ttttaattca ttgaggcgtt agccacagga gggatctatg tccgggtttt atcataagca
     1261 tttcctgaaa ttactcgatt tcacgccagc tgaactcaac agcctgctgc agttagccgc
     1321 gaagctgaaa gccgataaga aaagcggcaa agaagaagcc aaactcactg gtaaaaacat
     1381 cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc tctttcgaag ttgccgcata
     1441 tgaccagggt gctcgcgtta cttatctcgg cccaagcggc agtcagattg gtcataaaga
     1501 gtcgattaaa gacactgccc gcgtgctggg tcgcatgtat gacggtattc agtatcgcgg
     1561 ctatggtcag gagattgtcg aaacactggc ggaatacgct ggtgtgccgg tatggaacgg
     1621 cctgaccaat gagtttcacc ccacgcagtt gctggcggat ctcctcacca tgcaggagca
     1681 tttacccggt aaagcattca acgaaatgac gctggtctat gcaggtgacg cacgtaacaa
     1741 catgggcaat tcgatgctcg aagctgcggc gcttaccggt ctggatttgc gtctggtcgc
     1801 gccacaggca tgctggccgg aagctgcgct ggttacggaa tgccgcgctc tggcacagca
     1861 aaatggcggg aatattacgc taacggaaga tgtagccaag ggagtagaag gtgctgactt
     1921 tatctatacc gatgtgtggg tgtcgatggg ggaagcaaaa gagaaatggg cagaacgcat
     1981 tgcattactg cgtgattatc aggtgaacag caagatgatg cagttgaccg gtaacccgga
     2041 ggtcaaattc ctccactgcc tgcccgcttt tcatgacgac cagacgacgc ttggtaagaa
     2101 aatggcggaa gagtttggcc tacatggcgg aatggaagtg actgatgagg tcttcgaatc
     2161 tgccgccagc attgtttttg atcaggcgga aaaccgtatg cataccatca aagcagtgat
     2221 ggtcgcgacg ctcagtaact aattgatatc gctataaaga tgccgggaaa tacaaaacta
     2281 tcccggcatc acattatttt atgcagtgat aggtctattc ttccacccaa ttttttctat
     2341 tgcagaacgt aaatcttgcg gtggatttaa tttttctaac tatttttcat t
//
LOCUS       DAZUGK010000359         2368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZUGK010000359 DAZUGK010000000
VERSION     DAZUGK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.361"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..207
                     /gene="xisR"
                     /locus_tag="ACHY5W_004758"
     CDS             <1..207
                     /gene="xisR"
                     /locus_tag="ACHY5W_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS4449484.1"
                     /translation="EEQLIALKGIKKGTLKKAREKSFMEGREYKHVAHDCMPWDNSPC
                     FYNLEEIDRWIERQASARPRRHLT"
     gene            227..1522
                     /locus_tag="ACHY5W_004759"
     CDS             227..1522
                     /locus_tag="ACHY5W_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFS4449485.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            1700..1942
                     /locus_tag="ACHY5W_004760"
     CDS             1700..1942
                     /locus_tag="ACHY5W_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS4449486.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(1953..2024)
                     /locus_tag="ACHY5W_004761"
                     /pseudo
     CDS             complement(1953..2024)
                     /locus_tag="ACHY5W_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            2109..2192
                     /locus_tag="ACHY5W_004762"
                     /pseudo
     CDS             2109..2192
                     /locus_tag="ACHY5W_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
BASE COUNT          750 a          514 c          507 g          597 t
ORIGIN      
        1 gaagaacagc taattgcact gaaaggaata aaaaagggaa cgctaaaaaa agccagagaa
       61 aaatcgttta tggaaggaag ggaatataag catgtcgccc atgactgtat gccatgggat
      121 aacagcccat gcttttacaa cctggaagaa atagatcgct ggattgaacg ccaggcatca
      181 gcaagaccaa ggcgtcatct tacttgagta aaaacaatac taaccaatga gagaagctga
      241 aatgaaatat ccgacaggcg tggaaaacca tggtgggaaa ttacgtatct ggtttgttta
      301 taaaggtgta agagtccggg aaaatcttgg ggttcctgac acagcaaaaa acaggcgcat
      361 tgcaggtgag ctacgcgcct ctgtttgtta cgcaataaaa actggcgctt tcgactatgc
      421 aaaacagttt cccgcctcac acaatctgga aaaatttggt gaggcccgac aagatttaac
      481 cataaaagaa ctggctgaaa aatttctggc actgaaagaa actgaagtcg caaaaacgtc
      541 actcaacacg taccgtgccg tcatcaaaaa tattctgagc ataatcggtg aaaaaaatct
      601 tgcatcatcg attaataaag aaaaattgct ggcggtacgt aaagagttac tgactggata
      661 ccagatcccc aaaagtaact atattgttac acaacccggg agatcggctg ttaccgtaaa
      721 taattacatg acaaatcttt acgccgtgtt ccagtttggt gttgataacg gttatctggc
      781 agacaatcca tttaagggga tctcaccatt aaaggagtcg agaaccatcc cggatccact
      841 ttcgcgggag gagtttatcc gccttattga cgcgtgcaga aatcagcaag ccaaaaattt
      901 atggtgtgtt tccgtttata ctgggattcg ccctggtgaa ctctgtgcgc ttggatggga
      961 agatatagat ctgaaaaatg gaacaatgat aatcagaaga aatctggcaa aagaccgttt
     1021 tacagtacca aaaacacagg cgggaaccaa tcgtgtgatt caccttatta agcccgcaat
     1081 cgacgctctc cggagtcaga tggcactaac gagactgagc aaagagcata tcatcgatgt
     1141 tcacctcaga gaattcggca gaacagagaa acaaaaatgt acctttgttt ttcaacctga
     1201 agtgtcagcg aaagtaaaaa attatggcga ccattttacc gttgactcaa taaggcagat
     1261 gtgggacgca gcggtaaagc gtgccggaat ccgccatcgc aaatcgtatc agtcgagaca
     1321 cacttatgcc tgctggtcgt taacagcagg agctaacccg gcattcattg caaaccagat
     1381 gggccatgca gatgcgcaaa tggtgtttca ggtgtacggg aaatggatgt cagaaaataa
     1441 taatgcgcag gtaacgctgt taaatacaca gttaagcgag tttgccccaa ccatgcccca
     1501 taacgaagcg atgaaaagtt aattttttat ttatcaatta gttagattga atgactcttg
     1561 aaatccataa ttcacaggtg ttttttcaca tcctgtgggt tccttggcgt tttctacgtt
     1621 ttttcagata gttgcatttt ttctaaaaat ccctaatctc gattttgctg tttatttgag
     1681 gcctttttat gtcccatata tgccccacag ataccccgca gccaaaatca acaaaatgcc
     1741 aaaaggttct gttcctgccc tgcaacaaga aatgctgcga cgtgtcagta aacgttatga
     1801 cgatgtagaa gtgatcatca aatccaccag caacgatggc ctttcagtta ctcgcaccgc
     1861 cgataaagat tctgcaaaaa cttttgttca ggagacgctg aaagatacct gggagtctgc
     1921 tgacgagtgg tttgttcgct aataaacacg taaatcggta acggctggaa atcattcaat
     1981 actcgcacta tcgaaagttc accagccaac cgcgacacgc tcttacatac gaagtgccgc
     2041 gctttcctta ataatttttt agcagtactg tgtaaatgat gagcgaccta atccatgcat
     2101 gacgcgtagt gcctattgtg catctttctg cgtctctttt tactggaccc aagccaatga
     2161 tgctggcgat accattcccg ccaggcccac ttacaacagt aaaattcgga gctctgcctt
     2221 tacatacaat acaatgttgc acgattgccc atcataaact ccgttaatat ctatgagtag
     2281 taaccctatg taactgtaaa gacgaatcag cgctaattgt gtctgtccac atccccaaat
     2341 gtaaagatat aaggagcacc atgataca
//
LOCUS       DAZUGK010000360         2337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZUGK010000360 DAZUGK010000000
VERSION     DAZUGK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.363"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..887
                     /locus_tag="ACHY5W_004763"
     CDS             <1..887
                     /locus_tag="ACHY5W_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS4449487.1"
                     /translation="MFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKE
                     QLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLP
                     FDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALL
                     NKFAVFPSDYISDCANHSSSKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIM
                     LASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKK
                     CRWPIRNVPWGASSVKYS"
     gene            complement(1004..2329)
                     /gene="gdhA"
                     /locus_tag="ACHY5W_004764"
     CDS             complement(1004..2329)
                     /gene="gdhA"
                     /locus_tag="ACHY5W_004764"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4449488.1"
                     /translation="MESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSL
                     LERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKF
                     LGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPA
                     GDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLK
                     RHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARL
                     IEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGV
                     KAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKV
                     DARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKIADAMLAQGVI"
BASE COUNT          592 a          584 c          570 g          591 t
ORIGIN      
        1 gaatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
       61 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
      121 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
      181 agaaattaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
      241 aaacgctgga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
      301 tgcttccttt tgactcgatc gatatcataa gtaaaaggcg cgagtcttta gaggatcaat
      361 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
      421 atggagccaa tacctttaaa gcgaacatgg aggaagctcc agagcaggtc atcgccttgt
      481 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat catagctcca
      541 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
      601 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
      661 cctctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
      721 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
      781 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
      841 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaatat
      901 tgccgggggc gctgcgcttg cccggcctac attagagcgt aactgcaatg aattatgccc
      961 atttgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
     1021 ccagcatcgc atccgcaatc ttcacaaaac cggcaatgtt cgcgccctgc acgtagtttg
     1081 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
     1141 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
     1201 ccaggcctga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
     1261 ctgcctgctg gaacagttca gttgcttcga tggtggtcgg catatttgcc ccttcggcga
     1321 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcattctggg
     1381 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
     1441 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
     1501 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
     1561 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
     1621 cgttgccgga gccagaaacg gaaacgcgca ttccttcaaa acccataccg tggcgtttta
     1681 gcattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactgc
     1741 cgccaaatga aagaccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
     1801 tcatccccgc cataaagccg acttcacgac caccaacccc gatatcacct gccggaacgt
     1861 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgcctggcag aaacgcatca
     1921 cttcgccttc gcttttccct ttcgggtcga aatcgctgcc gcctttacca ccgcccatcg
     1981 gcagagtagt cagggcattt ttgaaggttt gttcgaagcc gaggaatttg agaatggaga
     2041 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
     2101 gcacacgcca tgcacggttg acctgtacct gattgcgatc atcaacccat accacgcgaa
     2161 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
     2221 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
     2281 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatg
//
LOCUS       DAZUGK010000361         2335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZUGK010000361 DAZUGK010000000
VERSION     DAZUGK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.364"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..916
                     /gene="yahL"
                     /locus_tag="ACHY5W_004765"
     CDS             101..916
                     /gene="yahL"
                     /locus_tag="ACHY5W_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFS4449489.1"
                     /translation="MISLKAPRNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1329..1574
                     /gene="yahM"
                     /locus_tag="ACHY5W_004766"
     CDS             1329..1574
                     /gene="yahM"
                     /locus_tag="ACHY5W_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFS4449490.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1591..2262)
                     /locus_tag="ACHY5W_004767"
     CDS             complement(1591..2262)
                     /locus_tag="ACHY5W_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFS4449491.1"
                     /translation="MMKLLHLFMDEITMEPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPCYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
BASE COUNT          693 a          499 c          519 g          624 t
ORIGIN      
        1 acaatacttc tcacgttagt tatgagcgat ttctgatagt gcctggttta attagagctt
       61 tattatctgc gacgtttatt tttatttaag agagatcgcg atgatatcat taaaagctcc
      121 gcgcaataat ttgatgccat atacgcaaca aagcatactt aatacggtta aaaacaatca
      181 gttaccagag gatatcaaaa gctccctggt ttcctgtgtc gatatattca aggttttgat
      241 taaacaatat tatgattatc cttatgattg tcgtgatgat ttagtcgatg acgacaaact
      301 cattcatctc atggctgccg tacgagactg tgagtggtca gacgataacg cactcaccat
      361 aaatgtgcag tttaatgatt tccctggatt ttatgactgg atggattacc ctgatcaccc
      421 ggttaagttt gtttttcgca tacttgaaaa tcaaaaaggg acagtttggg tatatgacca
      481 ggatgatgcg tttcttgata ttaaagcaaa tgtgcaggct ggacgcttta ccgggcttaa
      541 gaaattagtg cagtttatcg attcagtgcg aacagattgc aaatgtattc ttcttgaaca
      601 ccatatgcct ttgctgagaa ttttccctaa agggaaagag tgtatgcatg tggaaaaatg
      661 gcttcgcgaa atgtcgtcta ttccggagac agacgcgccc attaagcagg cgcttgcgca
      721 tggattatta ttacacttaa aaaatatcta ccctgttttt cctgaatcac tggtgatgtt
      781 attgctctct gttctggacg taaaaacata cagagatgat gcgcgattaa atcaatggat
      841 atctaataga gtccaggaat taggtgacag atattatcct gtaaataagc acgttaaaat
      901 ccgctacact ctttaggtca gaaagggttc cacctgcaag ttgttattcc accagagagt
      961 gaatcgcaat gcaggtaatc atttcatctg tatatccgta tgccaaatgg cattccaccg
     1021 cctttttcgc cgatattttg ttgtgatcgg cgacgcttcg cttaaaaaag caccagctgt
     1081 ggttttgcag ctatgcggtg tataaaaaat gatctcatgc agatgttttg tgaatgtgtg
     1141 ggttgacatt catatgaaaa aaatcataat tccatcatgt ttgtgtgggg tcttttctgt
     1201 accttacgca tcgcactcaa gcctgacaga aaatatgctg taaggctcat atcaaaaacc
     1261 gccattagct catcaggaag agcagacgac aaccaggatt gttgtatggt acggggttcg
     1321 aggcctcgat ggcggtccaa cttttcaaaa ctcttctgaa tcagatcccc ttattatcat
     1381 ccctgcaaag tggaacattg ccattgtttg gctattcagg acgggtcaga ccaatgaaaa
     1441 aagcccacac aggggagagt gggctgaaat gggaagctaa agactcaagt aaacttatcg
     1501 gaaataagga ccacgcatta cgggggctat catcgccgat ggtcgggatt cggcaaatca
     1561 ggttgattac gtagagagta aattatctgc tcaccgctgc gtcacccctt cgtaaatcag
     1621 gcgtagcgcg aatacaccaa taattgcacc aataacccga ctggcaacgc gttgcatacg
     1681 cccataagca cgacgcacag cgggcaaaga aaacgcctga ctaagaaaga ctcgccagat
     1741 aactgatgcg agtacaatcc ccgcccaggc cattaaacgt gcccatgttg gtgtttcggc
     1801 atttaatgtt actgagaaaa tactaataaa aaataaaacg gtttgcgggt tagagagatc
     1861 ggtaattaat ccacggcgaa aaaagacata gcagggggcg ttaatcggtt gttgtagtgt
     1921 gctcatttgc ggtgttgact ggcggcgcat gctgcaccac gcaaaccata agagataagc
     1981 gccgccgacg attctgataa gcgaaaaaat ctcctcacac tgcgtaatta gcgttgcaag
     2041 accaaacaaa cccaacccgg aataaaatgc atcgcccagc gccacgccca gcccggtcag
     2101 cacccctgcg cgtcgaccgg aagccaggct ggtttgtact accacaaaga gattggctcc
     2161 cggattaaaa aaagtaatca cgaacagtcc cacggtcagg taaacggcgt gcaaaggctc
     2221 catagtgatt tcatccataa ataagtgaag taacttcatc atatttctac caaaaataat
     2281 cgggtgcgag agagatcaca aagtgtctta tttccggtta ctggcgttta tgccc
//
LOCUS       DAZUGK010000362         2322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZUGK010000362 DAZUGK010000000
VERSION     DAZUGK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.365"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..664)
                     /locus_tag="ACHY5W_004768"
     CDS             complement(<1..664)
                     /locus_tag="ACHY5W_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449492.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKN"
     gene            complement(666..944)
                     /locus_tag="ACHY5W_004769"
     CDS             complement(666..944)
                     /locus_tag="ACHY5W_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449493.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(1112..1267)
                     /gene="hokD"
                     /locus_tag="ACHY5W_004770"
     CDS             complement(1112..1267)
                     /gene="hokD"
                     /locus_tag="ACHY5W_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4449494.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1872..>2322
                     /locus_tag="ACHY5W_004771"
     CDS             1872..>2322
                     /locus_tag="ACHY5W_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4449495.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVL"
BASE COUNT          605 a          548 c          550 g          619 t
ORIGIN      
        1 tattttttgt gcgctgcttc agtatgctgg tggcggtctg ttctcccggt atgatgtcgc
       61 tttcacggta tacggagcgg attttttccg ccggtaatcc cagagaacga cgcgatactg
      121 cctcaggtaa tgcgtccacc acctgattgc agactgccca ccaggataat tcagccagcg
      181 ataattcccg ttcctgtgtg ccattcattg cgtggcggat gacgtcaatc atccatgctg
      241 acaggttttg ttgagcaagt tgctcaagtg attcggatgt ctggtcgcgc agctggttgt
      301 cgcagtgcca gcacaacacc atcgcgccgg taccgtaacg atgtatgacg gtttcgctgt
      361 ggtggtaatc accatgaggc cactggcagg atttaacgtg acgtaataac cagtcagaca
      421 gtgcaccagc gccaccagca gcacgaatca cacgctcatt gctgaaaaat ggcagtaatg
      481 atttatcttc cgccagcggc tggcgaacgg caggaacgac cccggacggc agattacgca
      541 tgcttttcgg ttcagactcc accagcaccc tgccgccatg aaatacccgc atggattcac
      601 ggcctggctt aacgataacc agaccgagtt ccggtaccag aacaggtcga agtaataccc
      661 gcacattacc tccagatccg ttgctggaat gtgcgggacg gacgcggtgg ccgttcggag
      721 taagggagcc tgacggagat tatccagtga cggtagtcga ggctaagggc ttttttaacc
      781 tcgcatccgc gcctgcggta acactgaatg agccattcgg cctgttcttc agtgcatggg
      841 ggatgctggt accagtcaga tttgaatgcg tgaaaacacc gtccgcgcct gctggcaaag
      901 acggcagaat catcagaatt gtataatttg gtatcgtgcg ccatcggttg tctctgctgg
      961 cgcagcaggt gccagttgtt caggctggcg tgcgaattgt aaaccagaat gccaggaaaa
     1021 aacaaaaccc gccgaagcgg gttaagtgcg ggtgcgttga ggatgcctga ttcatcagag
     1081 gtggcgaggg atttctcccc cgcctggtct cttactcctc aggttcgtaa gctgtgaaga
     1141 cagcgacctc cgtctggccg gttcggattc gtacctcgca gaggtctttc ctcgttacca
     1201 gtgccgtcac tatgacggtt aaacagatga cgatcagggc gattaacatc gccttttgct
     1261 gcttcatagc ctgcttctcc ttgcctttcg gcacgtaaga ggctaaccta gatttgccgt
     1321 tcatagattg agcctcagat taatgttaag cgtcttgcag gacgcgtaat gttaactggg
     1381 gcttttctct atctgccgtt ggtgttcatg cccgaggcag atagcctcaa gcacccgcag
     1441 ctattctact taacttccca ttacctcgcc aatatgaaat cggtcagaaa gacagacgtc
     1501 ccataaggac aacaacggaa aaagagattt tgcttgatgc tatttacctc tgcaccatga
     1561 agttgcgcca aaatcccagt ggtgataaca ggaatttggt tgttggtgat tggggaaaga
     1621 agtacgttag actgtgggtt agttcattga catgatagtt acctgccttc aatttcctaa
     1681 gcaagcgatg taaacgataa tcaatatcaa atgtattgac aggtaaaata ataacgatgt
     1741 acctttgctg ataacatata tcagtcttaa atcataatgg aggtagataa aataactaaa
     1801 cacacacaac aaatttttta tcagtgacat agcatatctt aagcatcctt taatgctcat
     1861 ggggagaacg catgactaaa catatggaca ttcattgtca aacacgcata tttaactcaa
     1921 ttaaccatga caccaaatat aaaatatgta tttttactcc aaaaaacatt aaaaaaaata
     1981 atgcaactct ttatatactt gatgggaata gtgccaacaa ttacatttct gatattctgc
     2041 ctgttattga tgcactaccc aatccaccag tgttagttac acttggttat gaatcttgga
     2101 ataaccttag cattcatcgc cgtgcttacg attatactcc tgatggtgaa aatgctattg
     2161 ttgataactc taaaccagca tggatctact ttactggtgg aggaagccag tctttccgag
     2221 aattattact aactcagatc atgccatggg ttagtactat tgctccgaac agttccagaa
     2281 tcggtatatg ggggcactcg ctaggtgcta tttttgtgct tg
//
LOCUS       DAZUGK010000363         2321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZUGK010000363 DAZUGK010000000
VERSION     DAZUGK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.366"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            104..>2321
                     /locus_tag="ACHY5W_004772"
     CDS             104..>2321
                     /locus_tag="ACHY5W_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4449496.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDESTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGSLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHSGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFSSGKLSGFDMGYSGIQFGGDKRLSDEM"
BASE COUNT          640 a          568 c          571 g          542 t
ORIGIN      
        1 ttgcaccata caaatatcat gatgataaga ggatattatt cattgactta aacaaaatga
       61 caaaatccat agccccatag cctgggtact atataggagt ggtatgggca tcaaacaaca
      121 caatgggaat accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca
      181 actgataaaa tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc
      241 tgctgataca ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac
      301 aggagatacc gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac
      361 gcctggcaat atcaacttgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata
      421 tgcaaaagga atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt
      481 agatgttgtt ggccaaacct ctgccatcgg cattaattta attggtgact atacccatgc
      541 tgacttaggc acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag
      601 ctcaacacta acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat
      661 caatgactat ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatgaaag
      721 tacaggtgtt tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac
      781 ggcgacagac ctgacaatcg atgttcaggg atacagcgcc atggggataa acgtacagaa
      841 aaactctgtt gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca
      901 cggcctctgg agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc
      961 cgcggccaat ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat
     1021 ttcttccgcg cagggcggta gtctcgtagc cagtggttca gacgcgacaa tcaatttttc
     1081 tggcacggca gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca
     1141 gacggcaacg gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag
     1201 tctggcgctg ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat
     1261 caccggcgcg gcaggagcca gggggattta tgccatgacc aacagccaga tcgacctcac
     1321 gagcgatctg gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga
     1381 cgatggttat gccgccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt
     1441 tctttccaaa ggtgggctaa tcaatctgga tatgcactct gggtcggttt ggacaggttc
     1501 ctccctcagc gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg
     1561 gaacgtaaca agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt
     1621 tgccagccac gcatcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg
     1681 taacagtacc tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataataa
     1741 aggggattta ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa
     1801 ccagggcagc gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg
     1861 tgcggcctcg ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg
     1921 taaaaatggc actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa
     1981 tcctgaacca acaccggctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctga
     2041 acccgatcct acgcctaacc ccactcccac acctaaaccc accacgaccg cagatgctgg
     2101 cggcaattat ctcaatgtcg gttacttatt aaactatgtt gaaaaccgta cgctgatgca
     2161 acgaatgggt gacctgcgaa atcagagtaa agacggtaat atctggctgc gcagttatgg
     2221 gggaagcctg gactccttct ccagtggcaa actgagcggc tttgacatgg gttacagcgg
     2281 tatccagttt ggtggggata aacgtctctc tgatgaaatg c
//
LOCUS       DAZUGK010000364         2287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZUGK010000364 DAZUGK010000000
VERSION     DAZUGK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.367"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..514)
                     /locus_tag="ACHY5W_004773"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="ACHY5W_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(519..683)
                     /locus_tag="ACHY5W_004774"
     CDS             complement(519..683)
                     /locus_tag="ACHY5W_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449497.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(680..1414)
                     /locus_tag="ACHY5W_004775"
     CDS             complement(680..1414)
                     /locus_tag="ACHY5W_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4449498.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPEETTTATEAKISECDLTATIEQRGPQTADELATLFGTT
                     SRKVASTLAMAISKGRLIRVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGV
                     ALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKW
                     ERVCAALRELNKHRDMVAGICRKSGQ"
     gene            complement(1448..1909)
                     /locus_tag="ACHY5W_004776"
     CDS             complement(1448..1909)
                     /locus_tag="ACHY5W_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS4449499.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1902..>2287)
                     /locus_tag="ACHY5W_004777"
     CDS             complement(1902..>2287)
                     /locus_tag="ACHY5W_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4449500.1"
                     /translation="WGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNH
                     VRAQVKPVSKGVNHAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGG
                     LFEPLDPEERGRTIEALDCTDWRDD"
BASE COUNT          473 a          683 c          508 g          623 t
ORIGIN      
        1 ttgtaatcaa catcctgttt ggaatagcca gcctttaatg gataactatt tctgacaatg
       61 caatgagtat aatcaagtcc atcttccact gagaattaga ggcggcatgc tttttccggc
      121 tcttgccgga tatccgtaat tgtccataat ctgcagattt ataccttctg catgacctgt
      181 cagcgaaaat ttgtccggtg tttctacggg aatgacatca aagttacacg cactcgcgtt
      241 accgtgtaga cctactttcc tgcacttgca agatcacagt ggtgtaaccg taacaggaat
      301 ttattctctg gaccggcagt aaatccctga gtggcgtggt tcccatatca atttcccgcc
      361 aggcagcctc cattgccagc gtacaggctg gagccatgac ctgcccttta aatctggccc
      421 gaccatccca ccggacgtgt tcttctcccc tgaacttagg tacagtcatc tccagtggca
      481 caaaagtgtc agcgccataa tttttgaccg ttatcgcgct acggatattt tgttgactgg
      541 tgaaaatcac cccgcagaat caggcttatt cccttaacct gggctttcat cctgaccgcc
      601 gcctcactac gaccaatcag actgccgatg cattttacct tcattgttag tatcatgatt
      661 tcaggcctgc accatccgct cattgcccgg acttccgaca aatcccggca accatatccc
      721 ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc ccacttctgg acatgattct
      781 tcgccctacg cagttcgcga tttgccatat gcagcgatgg caaaatcaaa tcatcttctc
      841 gcgttgcagt aaacgatggc agcgactgca caatgtccgc cacagtttct gttttaatat
      901 cttcctgtgt tgcatctttc tgtaccggta acgcaacacc ggctggctga ggaaaggctt
      961 taccatcggt ttccgctacc gattcaactt tcggctctgc tggtaaatta tcacctggta
     1021 tgcagtaacg aattttaccg ttctggttta cgcgaatcag acgacctttg ctgattgcca
     1081 ttgccagcgt tgaagcaact ttgcgggatg ttgtaccgaa cagcgtagcc agttcatccg
     1141 ccgtttgtgg tccgcgttgt tcaatcgtcg cggttaaatc gcactctgag attttcgctt
     1201 ctgttgccgt ggtggtttct tctggcgctg gctgttcctg ctgaacgttg ttatcagcca
     1261 cacgccaggt gtatacgctt ttatcaacga agccagcctt tttcagttcc cacagctcgt
     1321 tcagtacttc ttcacgactg atatcaagtc gcgcagccag ctctaccgac gtggcttttc
     1381 ccatcgcttt cagtgcgtca aaaacagtct ccataaattt cctcccggta aaaaatcact
     1441 tctcaactca aacaaaacca gccgctttcc ggcgttcata ttcctgtttc agcaactcaa
     1501 ttggcgttgg ccccgacggg cgtttggggg ccgccagttg tcgccggact ggcggaacgc
     1561 tcaggccgtt actaacatgc tttgcccatt tcgtcagttg ccgttctgca agccgtttta
     1621 attccccttc ggtcatctgg cgctcaatcc cctttgaacg catctcgagg caaatgtgat
     1681 acagcacagg ctgagaccac gggtacttat cacttccgtc gtatcgccag gactcattgc
     1741 gccagcggcg gtactcctcc atcacagcat ccaccgtcag accgaatgga ttggctccgc
     1801 ttttcgaaat cagtgccaca aactcagcca ggtccggagg ccatgtttca cccgcccggc
     1861 agcggtccat gcactggcgg cagacctgcc ggatttgctg ctcagtcatc gcgccaatct
     1921 gtgcaatcca gagcttcgat ggtgcggccc cgttcttctg ggtccagcgg ttcgaataaa
     1981 cctcccccat gagttcccac agcttccagg ccgtttccgt cgctgataaa tccgttttca
     2041 cgttcccact gctcacgtgc tgcccgaatt tcctgaactg cccgtgatgc ggtgccacct
     2101 ggtgctgctg catggtttac ccctttgctg actggtttaa cctgcgccct gacgtgattt
     2161 acgtgacggg cgaatttctg ctcccactga atctgcgtaa acactttccc ctccgctgcc
     2221 cagtagtccc ggaaggcggc aagttcagca ggtgtaaatt ctgtctccgg caaagccatc
     2281 ccccaca
//
LOCUS       DAZUGK010000365         2267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZUGK010000365 DAZUGK010000000
VERSION     DAZUGK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.368"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..116)
                     /gene="escO"
                     /locus_tag="ACHY5W_004778"
     CDS             complement(<1..116)
                     /gene="escO"
                     /locus_tag="ACHY5W_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFS4449501.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVN"
     gene            complement(176..1516)
                     /gene="escN"
                     /locus_tag="ACHY5W_004779"
     CDS             complement(176..1516)
                     /gene="escN"
                     /locus_tag="ACHY5W_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFS4449502.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1500..>2267)
                     /gene="escV"
                     /locus_tag="ACHY5W_004780"
     CDS             complement(1500..>2267)
                     /gene="escV"
                     /locus_tag="ACHY5W_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFS4449503.1"
                     /translation="FEAGHAEISFEFVQEKLSTNSIVYKTNKTNQQLAHLTGMDVYAT
                     TNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQ
                     RLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVSGTLLNVW
                     LIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPTGNGVILTALDIRRY
                     VKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          645 a          550 c          426 g          646 t
ORIGIN      
        1 aaattcacca gcgatgcaca atatgcctgc ttagaggcaa tcgactcttt aatggattgc
       61 tcgcagtcat cgagtttacc atccacctct tttatctgag agttaatctt agccattgat
      121 tctctcaatc ggttcgctct gcttttacga atagataaaa ttctgtccaa catactcagg
      181 caaccacttt gaataggctt tcaatcgttt tttcgtaact actgatatct ttggtgcttt
      241 gttgtataaa gttctgaatt aatttcctat tttttattgc cttatctgct tcaggatctt
      301 gccccatcgt gtactcacca atacgaataa gcagctcaac atttttataa gttgcaataa
      361 gctttttgca ttctgccgcc gcacgcaaat gctccgatgt aacaacgtta tgcataacac
      421 gactggcact taaaccaata tcaattgcag ggaaatggtt ttcctctgca agttctcggg
      481 taagtacgat gtgcccatca agaatagagc ggacttcatc gccaatagga tcattaacat
      541 tatcgctttc taacaacacc gtataaatcg cggttattga accttttggc gcaggccccg
      601 ctcgctctaa taatttgggt aacgaagaaa aaacactcgg agggaatccc ccccttacat
      661 caggttctcc cgatgcaagc ccaacatcac gtgcggctcg ggcataacga gttacagaat
      721 ccatcattaa taatacattt ttaccttgat cgcggaagta ctctgcaata gtcgtcgccg
      781 taaatgcggc tttcatcctt tccagcgcgg ggcggtctga cgttgtgacg accagtacac
      841 atttagaaag cgtggattga ggtaagagcg cgaggaattc atttacttcg cgaccacgtt
      901 cgccgataag agcaaggaca ataatatctg cggatgcgcc attacaaatc atccccaaaa
      961 gcgtactttt accaacgcct gaaccagcaa aaataccgat acgctgccca ataccgcaag
     1021 ttagtagccc atcaattgcc ctgacgccaa gtataaatgg ctgatcaata acctgcctta
     1081 ataaaggatc tggtggttca gcatacaagc tgcgttcaaa cggaagatag ggggcagcaa
     1141 tattactctc cattggtctg cctatgccat caacaagccg cccaagtagt gcgtctccaa
     1201 cacgaatctt gaactcatct ccctgataac ttaaccactg accacagtac atgccggaaa
     1261 catgctcaaa aggcaacaag aaaacctcat cttcatcaat agcgatcacc tcggctaaac
     1321 gttgactggg ttctatctta taaaaggcgc caatacgcgc ttttggtaaa cgcgccttaa
     1381 taatcgtccc gccgatattg ataatcttgc cttcatatct ggtagacgaa tttaatgata
     1441 atgccggcac agtgctattg agcactttct gaatacgtgg gtatttttcc aatacagaat
     1501 catgctctga aatcatttac cgttcctaat actttaagtt cgatattatt cccaacctcc
     1561 tgaaaagaga gcacggggac tgacgggaac gaaccttcaa tcattttctt cacatagcgc
     1621 ctgatatcta aagcggtcag aatgacgccg ttccccgttg gattcatgat attttttaag
     1681 aagccaatta tctgctcagt tcgctccggt gagatattca gatacgaacc tgatgacgtt
     1741 tgtctgatcg actctcgtag ctcattttca atatcagagc caataagcca aacgttcaga
     1801 agtgtaccgc taacgctata gcgacctaaa atatgccgac gcagggcaat acgaacgtat
     1861 tcgcacaaga taaccacatc cttttctttt gttgaccaaa aaataagcgt ctcaaagata
     1921 gttctcaggt ctctaattga gacattttcc tctacgagac gttgtaggat gtcaacaatt
     1981 ttgctcaaac caagctggcg ctgcagctct ttcacaagct cgttatattt tctctccatg
     2041 atgtccatca aataacgcgt ttcttgtacg ccgatgaact ctttggcatt agacaataca
     2101 agtttcttta gcaaaaacgt tatcttatca tttgttgttg cataaacatc catacctgta
     2161 aggtgagcga gctgttgatt agtcttattc gtcttatata cgatagagtt agtcgaaagc
     2221 ttttcctgga caaattcgaa tgatatctct gcatgcccag cctcaaa
//
LOCUS       DAZUGK010000366         2262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZUGK010000366 DAZUGK010000000
VERSION     DAZUGK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.369"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..775
                     /gene="yqeH"
                     /locus_tag="ACHY5W_004781"
     CDS             143..775
                     /gene="yqeH"
                     /locus_tag="ACHY5W_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449504.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            complement(853..1032)
                     /locus_tag="ACHY5W_004782"
                     /pseudo
     CDS             complement(853..1032)
                     /locus_tag="ACHY5W_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(1022..>2262)
                     /gene="sctN"
                     /locus_tag="ACHY5W_004783"
     CDS             complement(1022..>2262)
                     /gene="sctN"
                     /locus_tag="ACHY5W_004783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFS4449505.1"
                     /translation="SLIEAELDDVSVGEVCEIYASWQANERIARAQVVGFRNGKTLLN
                     LIGSSVGLTRTAILKPTGEQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNLRLI
                     DELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGKTVLMHMLVNNTE
                     ADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSDFSSVDRCNAALMATTVAE
                     YFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTL
                     KGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYPAIDVLKSVSRVF
                     GQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNAENDFAMNARPKLTNWLKQ
                     SVNEKMPMSETLKELERIVK"
BASE COUNT          637 a          508 c          431 g          686 t
ORIGIN      
        1 atttttaaat gtcttaatca gaattatagc aaatacagat taatcgcaat ttttcccagc
       61 aacattacaa aatcatggtg tattgacatc atcaataacc aatgaaatta tgcaattata
      121 tacggatagg gaggttctta acatggggct ttgtagtcgt tataaaagtc ttacatgcaa
      181 tagttgctca atgcattgcc aaataatgcc agaagagtca ccgcgtttgc agtattgcgc
      241 aaactcgtgt ttttgtatgt ggcccgaaga aagttcatat tttaatcgtg gtgtggtgga
      301 aggtatctta acaaaaaacc acaatgcgag attaagcgga tatatttttg tcgatttttc
      361 agtaagtttt ttacgtctat tccttgaaaa agactggatt gactatcttg caagtactga
      421 catggggatt gttttagtca gcgaccgcaa tatgcaatcg ttagctaact actggcgaaa
      481 gcacaactct gcaatatctg ctgtcatata taatgatgat ggtcttgatg ttgccaatga
      541 aaagatcaga caactattta ttggtcgtta tttatcattt accagaggta acacattaac
      601 tcagatggaa ttcaccataa tggggtatat ggtttctggt tataacccat atcagattgc
      661 tgaagttctg gatatggata tccgtagcat ctacgcgtac aagcaacgaa tcgaaaagag
      721 aatgggtggt aaaataaacg agttatttat tcgttcacat tcggtccaac attaatactc
      781 aacgatctgc cagaatcata ctctgcttat acgggcatct attggaatgc ccgtatttta
      841 taaataagca gtaacttcgc cgagtaactc cttcatatct aacagtgact gcagattttt
      901 aagttgcata tcatacaggc gttccttttc ggctaacttt tctttttctg catttaactt
      961 ttgtaatgat gcttcacatg cagccaggct ctgcgctgtt cttctgataa gtcgattaac
     1021 tttacttaac aattcgttca agctctttta gcgtttcact cattggcatt ttttcattaa
     1081 ctgactgttt caaccaattt gtgagtttag gccgggcatt catagcaaaa tcattttcag
     1141 cattttgccc agcgcgatat tcccctaagt caataaacac ctgtagatct tccaaagttg
     1201 tgaggatctt ccttacccgt gcagcattat ctctgtgttt ttcatcagtg acctgaccaa
     1261 atactcgact cacacttttt agaacatcta ttgccggata atgaccttgc ccagcgagtt
     1321 tgcgacttag ataaatatgg ccatcaagga ttgagcgaat ttcatcaccc agggggtctg
     1381 attcgtcttc cccttccagt aacacggtat aaaacgcggt gatacttcct tttagtgttg
     1441 gtcccggcct ctccagtaag cgaggcaaac tgtcaaaaac agaagcagga taaccacgtc
     1501 tggcaggtgg ttcacccgct gccagtttca tatcacgtag tgctcgtgca taccgcgtca
     1561 ttgagtcgat aaaaagtaca actctctttc ctcgatctct aaaatactct gccacggttg
     1621 tcgccatcag ggcggcatta caccgatcta ctgacgaaaa atcagaggtg gcataaacca
     1681 atacgcattt tgccgcattt actgattgtt tcagcgattc cgcgcattcc gtaacttccc
     1741 taccacgttc gccaatcaag ccaataacaa acacatcagc ttcagtattg tttacgagca
     1801 tatgcattaa aaccgttttg ccacagccag ccgatgcgaa aatcccaaca cgttgaccaa
     1861 tgccacatgt cagtacgcca tcaatgactc ttatgcgtgt ctctagtggt gtattaataa
     1921 ctcgacgttc ctgatacgac ggtggcagtt catcaatcag gcgtaaattt cctctatcgc
     1981 ctggagtgtc cgggacaaac ctttccataa tttgaccaga ggcattcaaa accgaaccaa
     2041 gaaaggcatc actgatctgt atggttaact gctctcccgt tggtttcaaa attgcagtac
     2101 gtgtaagccc cactgagctg cctataaggt ttaataatgt ctttccattg cgaaagccaa
     2161 cgacctgagc cctggctata cgttcatttg cttgccagct cgcataaatt tcgcatactt
     2221 cacccactga aacgtcgtca agttctgctt caatcagaga gc
//
LOCUS       DAZUGK010000367         2257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZUGK010000367 DAZUGK010000000
VERSION     DAZUGK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.370"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(506..940)
                     /gene="uspF"
                     /locus_tag="ACHY5W_004784"
     CDS             complement(506..940)
                     /gene="uspF"
                     /locus_tag="ACHY5W_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFS4449506.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1081..2214)
                     /gene="ompN"
                     /locus_tag="ACHY5W_004785"
     CDS             complement(1081..2214)
                     /gene="ompN"
                     /locus_tag="ACHY5W_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFS4449507.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAFSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          617 a          487 c          521 g          632 t
ORIGIN      
        1 cgcggttggc tggtaagctt ttcgatagtg cgagtatcta atgatttcca gccggaactg
       61 attctattgg aaagaaatca tgggcgcaat ttacagatca cagattgatt agttatagat
      121 ttttactgtg gcaaaaatgg gacaaaaggc cgcaaaaggg gcaaaaatgg ggcaaaaaaa
      181 gtgtagatta ttgtgcttta ttgtagttgt taatcctgcc taacatattg aaaaacataa
      241 aagttattgt tcgtcagata gttgcggttt ttactctgac ttgttcaggt tgtattgttc
      301 tttcttgcta atttcttgat tttgcgacat ttaaaagcga ctcaattcgt tatatggcat
      361 cagaagagta tgcgtcatgc cggaacgccc agcataagaa atctgatata aaaactgtgg
      421 cgtgtatggt acggattaga ggggaaaatg tcagcacatt tgcgaaatga atcaaaaagc
      481 ccgcagcaat gtgcgggcgt tagtgtcagc gcacaaccag cacggagcac tctgcgtgac
      541 gcacgacagc tgcggcgttg gaaccgagca gataagtggt gatatccggt cgatgggaag
      601 caatgatgat catatgagcg gggatcttct tcgccaattc cagaatgcgg tctttgggcg
      661 agccttcctc aacatggaca tgcactctgt cggttggcag tttaaatttt ttaatgatct
      721 cttccagttg cgatttggct tccgctttca ggtcatccat tgccggtaat tctgcggaat
      781 acgctaaacc cagagaggca tagtagggca gtgaaggtat taccgtcagg aaatgaacct
      841 ctgcatcatc aatctttgcc tctgcctcaa cgtggctaat cacgcgttga gttaattctg
      901 aatcggaaat atcgataggg acaagaatcg ttctgttcat aaaacctcct gttttagtat
      961 ccgcataaag tgtaacgcca gatgacactt tttgtgtaat gacggagttc acatttttaa
     1021 tttagatcaa aggaggaaga ataagcagaa aaagcccgcc ataacagcgg gcaggaggat
     1081 ttagaactga taaaccagac ctaaagcgac aatatcatcg gtagagatgc cgttggcagc
     1141 gtagaaactg tcatcttcat ccaacaggtt gattttatag tcaacataag tggacatgtt
     1201 tttattgaaa tagtaagtcg cgccaacatc ggcgtattta accagatctt tatcatcaac
     1261 acctgccggg ttgtctgcac caccggcagc gtgcaggtca cggcctttag acatcaggaa
     1321 agagaacgcc ggacgcagac caaaatcaaa ctggtactgt gcagtgactt caaagttctg
     1381 ggttttgttt gcaacagcat aatcgctgtc gccaaacggg gtcatattac gcgtttctga
     1441 atacatggtt gccaggtaaa tattgttagc atcgtatttt agcccagcag tccacgcgtc
     1501 tgctttatca ccacccgccg cagtatggtt aacctggtca ttggtgcggt cagaagaggt
     1561 gtatgccgca ccagcgctaa agcccatgcc taaatcatat gttgtggaaa gaccccagcc
     1621 gtcaccgttt tcatggcgaa catcacgtcc gttgttggtg ccttcctgac cattactggc
     1681 accttcgttg ttaccttgat actgcaccgc gaagttcaga ccatttacca gaccgaagaa
     1741 atcagtatta cgataagtcg cgacgccgtt ggcacgacca gtcataaagt tgtctgcatt
     1801 ggtataagag tcaccgccaa attcaggcag catatcggtc cagccttcga tgtcgtacat
     1861 tacgccataa ttacgtccgt aatcgaaaga accgtaatct gcaaatttca gtccggcaaa
     1921 tgccagacgg gtccatgact ggttttttga agattcagtg ttgtttgcct gaatattgta
     1981 ttcccattga ccgtagccag tgagttgatc gttaatttgg gtttcgcctt taaaacccag
     2041 acgcgcatag ctctggtcgc catctttcgc tgaattatca gaaaaataat gcaggccatc
     2101 aactttgcca tacagatcta atttgttgcc gtctttatta taaacttcag ctgcatgtgc
     2161 agcacctgcg gcgagcaggg caggaattaa aagtgccagt actttgcttt tcattgaata
     2221 aatcctttag ttattttatt tgccttttat ccccatt
//
LOCUS       DAZUGK010000368         2230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZUGK010000368 DAZUGK010000000
VERSION     DAZUGK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.371"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..>2230
                     /gene="katP"
                     /locus_tag="ACHY5W_004786"
     CDS             129..>2230
                     /gene="katP"
                     /locus_tag="ACHY5W_004786"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFS4449508.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAV"
BASE COUNT          625 a          460 c          565 g          580 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt
//
LOCUS       DAZUGK010000369         2221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZUGK010000369 DAZUGK010000000
VERSION     DAZUGK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.372"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..567
                     /locus_tag="ACHY5W_004787"
     CDS             <1..567
                     /locus_tag="ACHY5W_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4449509.1"
                     /translation="GIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTAKNLCH
                     VHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLLKAESW
                     NEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIAVNLTP
                     AEVEGWRIYTVGELVKEIQTGAFTPATL"
     gene            791..1033
                     /locus_tag="ACHY5W_004788"
                     /pseudo
     CDS             791..1033
                     /locus_tag="ACHY5W_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            1107..1979
                     /locus_tag="ACHY5W_004789"
     CDS             1107..1979
                     /locus_tag="ACHY5W_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFS4449510.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVIGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTITLPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            2024..2203
                     /locus_tag="ACHY5W_004790"
                     /pseudo
     CDS             2024..2203
                     /locus_tag="ACHY5W_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          667 a          462 c          507 g          585 t
ORIGIN      
        1 ggtatctatg acaaaacaaa tagtgggtat gttgaaaact ggaaggaaat atctaaagaa
       61 atacgagaaa aggccaatta tgtttgtaat gattgtggcg tgaatttatc aaccgccaaa
      121 aacttgtgcc atgtccatca taaaaatggc atcaaatatg ataatcacca tgaaaacctt
      181 cttgttctgt gcaaggattg ccatcgaaaa cagcccctcc acgaaggtat attcgttacc
      241 caagctgaga tggctatcat tcaacgttta cgttcccaac aagggttatt aaaagcagaa
      301 tcctggaatg aaatatatga cctgactgat ccatcagtgc atggtgatat taatatgatg
      361 caacataaag gctttcaacc tcctgttcct gggttagatc ttcaaaactc agaacatgaa
      421 attattgcaa ccgtagaagc tgcatggcca ggccttaaaa ttgcagttaa ccttactccc
      481 gccgaagtcg aaggatggag aatatatacc gtgggtgagc tggttaaaga aatacaaacc
      541 ggagccttta cgccagcaac tttgtaaatt ctaacactcc gtgaaagtta agactttcac
      601 ggaagataaa taaaacttcc ctgatttgtg actcaaatta caaaagtagt ttatggcata
      661 acttgtctat ctttatggtg taataggtat aaaggcatat gctatggttc gcctcatact
      721 taaaacttcc ctcatacggg tgaaggttaa agctttgtag acaggagaca gtcacaatga
      781 ataaaacaat aaattgatgt cgcacgcgcc gatgggcatg gcgggtaatg tgacttttgt
      841 acacagcggc aaggcttatg ttactggcgg tgttaaccag aacatcttca atggctattt
      901 tgaagatctc aacgaggctg gagaatattt agcagctata gacaagatca atgcctatta
      961 tttcgacaaa aaagcagaag attatttctt caataagttt ttgttgtctt ttgatccctc
     1021 aacacagcaa tggatcttca cttgcaggct tgaacagcag aaaatttgcg agcatgattg
     1081 caattatttc actgcctggg atatcaatgg cttccagtaa atcgctacag caggcaattg
     1141 ccaacataaa aatttggcac aagggtgaac agcgcgcgcc gcataaacca ttgttactgc
     1201 tatacgtatt agcgggatac ctgaatggac atccgcgcct tttcgattat ggatcggaaa
     1261 tctacgaacc tctgcacagt ttactggaac gttttgggcc acagcgttca cagtaccgac
     1321 ctgatatgcc gttctggcga ttacaaggcg acggattctg gcaattacac aatgccgagc
     1381 gttgctcaac agcagggagc agccgacaac cgccagtgaa ggaactgaac gaataccacg
     1441 ttgcaggcgg tttcgatgaa cagcactacg ccctggtaat cggcaataaa aaactcatca
     1501 atactctggc gcaacagata ctcgaagcgc acttcaccga gagtattcag gaagaaatcg
     1561 ctgacgagct gggttttgat ctccagcaaa tccgtaaaca gcgcgacccg ttatttcgca
     1621 aaaatgtatt acgcgcttat aactaccagt gcgcgatatg tggctttaat atgcgccacg
     1681 acgacaccac cgtcgcgctg gaagcggcgc atataaaatg gaaacagcac ggcggtcctt
     1741 gtgaaattcc taatggcctg gcgctttgcg caattcacca taaagccttt gataaaggat
     1801 cgattggcct ggacgaaaat atgcgggtac tggtatcaga tgcggtaaac ggcggaggga
     1861 tcgttgagag attattctgg gattttgacg ggaaaactat tacgttgccg caggtgcgta
     1921 aaaattaccc ttatgaggtg tttgtagagt ggcatcggaa agaggttttt agggggtgag
     1981 gttgataact atcgcagtat gttcaatatt gctgacataa tcagtgcagg atcttgccgc
     2041 tagtttacaa ctcgccttcg atagcggcgc aaaacgtgtt ctgttgccga tatcctcggc
     2101 tatggatatt ccaacagttc cggcagagtt gtttaccaag tttcaggtaa gtttttactc
     2161 agaaccggtt gatgcggttt ataaggcgct ggatgtgaat taacacattc ctcagcttat
     2221 t
//
LOCUS       DAZUGK010000370         2200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZUGK010000370 DAZUGK010000000
VERSION     DAZUGK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.373"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(68..1591)
                     /gene="pdeG"
                     /locus_tag="ACHY5W_004791"
     CDS             complement(68..1591)
                     /gene="pdeG"
                     /locus_tag="ACHY5W_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS4449511.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHVGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(1923..2171)
                     /gene="ymgC"
                     /locus_tag="ACHY5W_004792"
     CDS             complement(1923..2171)
                     /gene="ymgC"
                     /locus_tag="ACHY5W_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFS4449512.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          658 a          490 c          464 g          588 t
ORIGIN      
        1 taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg gccttactga
       61 tcgattttca ctcaaccaca accttcacct tcggtttaga aaggataatt ttgaccaggt
      121 cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata ttattttggt
      181 tcagataatc aagttgttct ttcgtttcga caccttcagc gacgatactg atcgaaagtt
      241 tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct gcattgacgc
      301 ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatgc agataagaaa
      361 gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt tcatgcagaa
      421 tgttcaaccg ctgaaccaga ctttcatcaa catttaatgg ctcacgctcg gtaacttcca
      481 gaacgaggtt taaatcgcgg cgggtaaaac tgtcacggta atttaagcac tcgtcgacaa
      541 acgtcggcga aataatatgc gaggcgctaa aattaattcc aacatgaaaa ccttccggca
      601 atttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt tgcgtaagcg
      661 gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgaa atatatccac
      721 cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct tcccgaccat
      781 tcaccactgg ttggtaaaaa gggacgatct ccccattatt tatagcccgc cgcaggatct
      841 cttcaggcgt tgcgctttta ttgaaatacc tatctagcag gaaggcagcg gcgcaggcga
      901 ttaacagaat aaagatcaaa atcccaaagc ctttatcgat aagtctgtag aagctaaaga
      961 gtggcggcga gttaaagata atagtgaaat gatattttgt ggagtgaacc ttctcggtat
     1021 agggataatg tccattaagc gtcatcacat cacccgtagg tccaataatg tcatccgcca
     1081 cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga atatgctgat
     1141 cacttatcgt aaccaaaatg cgcgtatctg caaattggct ctgatagagc aggataggta
     1201 atctattaac ggtgtcgatt gccggagcca acagtaaatt actgtccggg aaaacaggaa
     1261 tacgagacag caggacccga ttccccggta aggatgtaca ccagaccgtt ccctgtttga
     1321 gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc tgatattgct
     1381 cctcgaggtc gcattccttc ccggcaatgt taaccgctgt ttgagtcgcc tgtgacgctt
     1441 cttcaagtat atgattgata ttgttggcgg catatcttgc tcccgccagg tactctgctt
     1501 ttgcggaata ccagagctgg atgttcaaaa tagcgacacc ggtaataaac aagcatatcg
     1561 caacgaggat gggtatgagt gtattgcgca tttgcttgtc ccggccaaag ggtttaatac
     1621 atgaaaatgc cattcattcg ctcgcgctgg catctacaga gagcatggta gatagcataa
     1681 gaaatatcca cgccgaagat aaaattttct ccatcttgat ctaacccatt aattattgag
     1741 cataatgtag gcagtataaa ataaattagg cgggatatca ggcgtcaaga atggagagcg
     1801 agaactctcc attcttgaca cctgatattg cggacatatt aagaaagcat aacgcctgaa
     1861 atgctcactt tgcatcagca tggtgataca gctgatgttt attctaaaac cttactcaag
     1921 ttctaagaga gcacggattc cctgtcatta ataatgatat ccgttagtgt agtcttatgc
     1981 atttcaatgt actgcaatat ttgcagatag agatcagaaa gaatcacact ggtttcatct
     2041 tttaaacgca agcgtaaagc ctgatcaata atatgactat caacataccc atgcgtcatg
     2101 aacacctccc tctcgagatt tttaaacaaa gcacattgaa aaataaatct ctctgggatt
     2161 gaattgttca tgtcagcctc gtagaaatat aacccaataa
//
LOCUS       DAZUGK010000371         2175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZUGK010000371 DAZUGK010000000
VERSION     DAZUGK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.374"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..1210)
                     /locus_tag="ACHY5W_004793"
     CDS             complement(join(33..952,952..1210))
                     /locus_tag="ACHY5W_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4449513.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT
                     "
     gene            1560..1775
                     /locus_tag="ACHY5W_004794"
     CDS             1560..1775
                     /locus_tag="ACHY5W_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449514.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1775..>2175
                     /locus_tag="ACHY5W_004795"
     CDS             1775..>2175
                     /locus_tag="ACHY5W_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449515.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACD"
BASE COUNT          499 a          535 c          565 g          576 t
ORIGIN      
        1 ttattaagga aaaaaacagc cagctgtagt ggtcaagtaa tattggccac ggttttacag
       61 taaaaatggt atctgttctc tgactcttcc ggcgtcagcc caccgttata atggtgaggt
      121 ctgacgctgt tgtagtagtt caatatgtaa tcattaattt gctgccgggc ctcgtccttg
      181 cctgcgtaac cattcgttgg cacccattct gttttcagac tgcggaagaa gcgttccatg
      241 gggctgttat cccagcagtt tccccgccga ctgacgcttt gatttattct gcaacgccag
      301 agaagttgtt gatatttaag gcctgtatac tggcttccct ggtcgctatg gaacacgaca
      361 tcccggggtt ggccacgcgt ctcataggcc atccgcaggg cactgcttat cagtgcggta
      421 tcggcatgcg ctgacagact ccagccgata accctgcggg caaaaagatc cataacaacc
      481 gccagatagc accagcgatt tcctgcccag agatacgtaa tatctccaca ccatacccgg
      541 tctggctccg gtactgcgaa ctgacgctca agcagattcg gcaggctggt atgctcctga
      601 cgggcatttt tatactgatg ttttccgggc tgacaactgc tcaggttcag atatttcatc
      661 agacgtccgg cacggtaacg gctcatcggg acgccgtttt gagtcagcat ttcagccaga
      721 gtgcgcgcgc ccgcagagcc ccgactttgg ttccacgccc ggcgtatttc gctgcacaac
      781 ctgactcgtg ccggattaac cgtatcgcgt cgttttcgcc agtaccggta actgctgcgg
      841 tgtatttcca gagcagaaca caggctgaca accgtgtggc tgtcacttag tctggcggct
      901 atcgtgaacc gttcagcgag tcggacatta agagcgcggt agcctttttt aatatcgtat
      961 tttgttcctc cagacggcga acttgctttt ccagctcgcg gatacgttgc tggtctggag
     1021 taatgggtgt ggcagagggc gtaataccct ggcgctctcg cctgagctgg cgtacccagc
     1081 tctcaagcgt ggttgaaccg acattcatcg cttcactggc ctgtcgatat gagtagccct
     1141 tatcaacaat cagctgtgca cattccagcc tgaactcagg ggtgaaggtt cgtttggttt
     1201 tcttgttcat taagtcacct gttttgtgtt gtggtgagaa tatcaccttt catcaggtgg
     1261 ccaaatttag tgtgccacta caagcactga ctttcggtgg agaggtgctg gctcaaaagg
     1321 atagatgtac ttcacatgtt gcttctatat ggcagtacat tttctgacag acagtgacgg
     1381 atgttgtcaa gatattgtgt catttataac ctgaatcagg ggaggccgga atgttatctg
     1441 gcatttttag cagagcctga atgccataat cacggctccc ggcgttggcc gtcagtgggt
     1501 gacactggcg gcttttttgt ttttctttac tttcattttc tgtcggcggt gacggagaca
     1561 tacatcagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg gtggggacgg
     1621 gatactggtt actgcagctg ctggacaaag tctctccgtc ccagtgggtg gcaataggtg
     1681 tgctgggaag tctgctgttt ggcctgctga cgtatctgac aaatctttat ttcaagatta
     1741 aagaagataa gcgtaaggct gcgagaggtg aataatgtcg ccgtcattac gcaaggctgt
     1801 tgcagctgct attggtggtg gggctgttgc catagcgtct gtgctcatca ctggtccagg
     1861 tggtaacgat ggtctggaag gtgtcagcta cataccatac aaagatatcg ttggcgtatg
     1921 gactgtatgt cacggacaca ccggaaaaga catcatgccc ggtaaaacgt ataccgaagc
     1981 agaatgcaaa gccctcctga ataaagacct tgccacggtc gccagacaaa ttaacccgta
     2041 catcaacgtc gatataccgg aaacaacgcg cggcgctctt tactcgttcg tttacaacgt
     2101 gggcgctggc aatttcagaa catcgacgct tcttcgcaaa ataaaccagg gcgatatcaa
     2161 aagcgcatgt gatca
//
LOCUS       DAZUGK010000372         2146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZUGK010000372 DAZUGK010000000
VERSION     DAZUGK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.375"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..193)
                     /locus_tag="ACHY5W_004796"
     CDS             complement(<1..193)
                     /locus_tag="ACHY5W_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4449516.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAV"
     gene            complement(203..1522)
                     /locus_tag="ACHY5W_004797"
     CDS             complement(203..1522)
                     /locus_tag="ACHY5W_004797"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4449517.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPTALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1503..>2146)
                     /locus_tag="ACHY5W_004798"
     CDS             complement(1503..>2146)
                     /locus_tag="ACHY5W_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449518.1"
                     /translation="APVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAA
                     GLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVR
                     RVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECA
                     KRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          397 a          690 c          605 g          454 t
ORIGIN      
        1 caaccgcaag aatgccaacg gcagcaccgt cggtggtgcc atcccacgca accagcttac
       61 ggctggaggt gtccagcatc agcggggtca ttgcaggcgc tttcgcactc aatccgccgg
      121 gcgcggttgc ggtatgagcc gggtcactgt tgcccagcgg ctggtaatgg gtaaaggttt
      181 ctttgctcgt cataaacatc ccttacactg gtgtgttcag caaatcgtta acggcatcag
      241 atgccgggtt acctgcagcc agcggtgccg gtgccccctg catcagacga tccagcgcag
      301 tgtcactgcg cgcctgtgca ctctgtggtg ctgcggccag aatgcggcgg gccgtttcca
      361 cggtcatacc gggggtttct gccagcacgc gtgcctgttc ttcgcgtccg tgagcctcct
      421 cacagttgag gatccccata atgcggctgt tttctgccgc aaccgctgcg gtgatctgcg
      481 cgttcacgtc cggctgcgcc gcgctggcgt tttcgccctc cgtcgctggc accacgtcag
      541 taacgtcagc ctgcgaagca gtggctgaaa cagttgttga ttgagtctct ttggtcattc
      601 gccctcctga gagacgggat ttacgtgcat ccagtgcatc acgcataacg gtgatcgcat
      661 cggtgctgtt gacaagttca tcagccagtc cggcatcaat ggcctcctga ccgctgtaca
      721 ctgcagcctc ggtatccagc acagcctgca cggacaggcc ggtatatgcc gacaccttct
      781 gcgcaaacat ccggcgggtt gcatccatcc gggactgcag tgtttcccgg acatcacccg
      841 gtagatggct gtaggggttg ccatccacct tatggctgcc gctgtaaatc agcgtgattt
      901 ccacgccctg tttctccagc gcagcaccgt aattactgtg agccatcatg acgccgatgg
      961 agcctgtccg ggcggtctgc gtgaccagac gccgggaggc ggcgctggca agcagctgac
     1021 ctgcactgca gttcatgtcg ttggccagcg cccataccgg ttttatgtct cgcacacggg
     1081 cgatgatgtc agcacagtca aatgctcccg ccaccatccc gcccggtgtg tccatatcca
     1141 gcagaatgcc gtccaccatc ggatcgctgg cagcctgttg cagacgggcg ataatgccgt
     1201 tgtaaccggt cattcccgaa tacggctgca gcgcccgcgt ccggctgacc agcgtaccgg
     1261 acaccggcag cacggcgatg ccgttcatga cctgataact gcgggcctgt cgtggtccgt
     1321 catcatcacc ggataacgcc agcgccgtgg gtgcctctcc ggcagtcagg ctgtcgccgg
     1381 acaccgcatc cgtcaggcgg ctgatcccaa gctggcctgc aagcgcacaa aagaaaaccc
     1441 gcgcataggc gggttcaagc atcagcggct cattaaaagc catgctggca atatgcggga
     1501 gattacgcag ctctgctgtc actcttctcc tcctctgttg attgtcgcag cccggattca
     1561 aatgccgcag ccgcccaggc gggcggttta agaccagctg cgcggcgctc catcgtttca
     1621 cggacctgct gggcaaaaat ttcctgatag tcgtcaccgc gtttcgcgca ctctttctcg
     1681 taggtgctca gtccggcttc tatcagcatc accgcttcct gaacttcttt cagaccatcg
     1741 atggccatac gaccggagcc tatccagtcg cagttccccc aggcactgcg ggcttcctga
     1801 aagctgaagc gcgcttttga aggtaacgtc accacgcggc gaacgatggc ctcttccagc
     1861 cagcacagaa acatctggct cgcctgacgg gatgcgacga attttcgccg ccccataaag
     1921 tacgcccacg actcgttcgc actggcccgt gccgtggagt agctcatctg ggcgtaattc
     1981 cgggaaagct gctcatacga gacacccagc ccggcagcga tataccgcag cagtgactgc
     2041 tcaaacacgg agtagccgtt atccgtgtcc tgagccgtct gcaggttcag tgagtccccc
     2101 ggcatcaggt gcggcacttt tgcgcctccc agacggaccg gtgctg
//
LOCUS       DAZUGK010000373         2115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZUGK010000373 DAZUGK010000000
VERSION     DAZUGK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.376"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            778..1755
                     /locus_tag="ACHY5W_004799"
     CDS             778..1755
                     /locus_tag="ACHY5W_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4449519.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            1939..>2115
                     /locus_tag="ACHY5W_004800"
     CDS             1939..>2115
                     /locus_tag="ACHY5W_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449520.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          569 a          520 c          504 g          522 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcagtgg ataaatgctt attacaacgt ttatcgacct
       61 catacaaaca atggtggttt atcgccttgc cgcatgaaga aaaatggaaa caggttattc
      121 cggtgtcctg attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca
      181 ttgcacatcc tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca
      241 ggcgacttct gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg
      301 cgaaccggcg aggaagcgca atgtatctgt agttacagtg agcagatacg ccctgaagct
      361 tcctcataaa acagttgtgt aaactgtttt cccggaatga tttatccaca tatccggcgg
      421 cccgatccag taaacagatc cataaatgat caagaaatga tccattaaag atccccatat
      481 cgctgcaaac cttgtcactc atgctccggg accacgatca cataagctgt ggcatgtttc
      541 tgataaactg tagcaggatc atgataagct gtattcagta atgtatatac tgtagtaagt
      601 taatgacata aattatggtc agtacgccag gctcagctgt taaatacaga ctgcaggttt
      661 ttcttcagtc agttgacggg gttctgacac tcaattttct gtttattctt ttactgtcca
      721 caggcaggag gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg
      781 gataagtcgt ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct
      841 gtggcgctga tgcgtctggg cgtttttgta ccgaccctta aatcactgaa gaacagtaaa
      901 aaaaatacac tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt
      961 gctgagggat ttgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc
     1021 aagacctggg tgggaatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt
     1081 gaactgcctt ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcccgc
     1141 aggctgcgtg agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt
     1201 tcccgcaccg atgagaagca cacccgggaa tatatcaccc atctggtaca gtcagcctac
     1261 tacgatactg aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac
     1321 cagtttgacc ggaaagtgct gctgcagctc aaggccatca atgccctgaa gcgacgggag
     1381 tccgcccagg cactttacac ctttatagag agcctgcccc gggatccggc accgatatcg
     1441 ctggcgcggc tgcgtgcccg cctcaatctg aagtctcctg tattttccca gaaccagacg
     1501 gtcagacggg caatggagca gttgcgcgag attggatatc ttgattacac ggagatccag
     1561 cgggggcgga caaaattctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat
     1621 gatgagagta aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg
     1681 gcggagaaac ttgccctgct tgaaaaactg ggcatcacgc tggatgacct ggaaaaactc
     1741 ttcaaatccc gctgaacata aactgtagtc agtgaagagt gctcctttac tgactacagc
     1801 ttatattatc aggtgcagtg agtggtctgc tcactgcagt ttatatgtaa gcgtacagcc
     1861 tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg
     1921 ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct
     1981 aattatcctc ccgaatttaa acagcagctc gttgctgcct cctgtgaacc cgggatatcc
     2041 atctcaaaac ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa
     2101 caatggcgcg aggga
//
LOCUS       DAZUGK010000374         2107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZUGK010000374 DAZUGK010000000
VERSION     DAZUGK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.377"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1190
                     /locus_tag="ACHY5W_004801"
     CDS             <1..1190
                     /locus_tag="ACHY5W_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFS4449521.1"
                     /translation="SSGGELDYLGEVSAEQLELVSSALKVIRTERVKKACTKCDCIVE
                     APAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIFARQGVELSRALLSNWVDAC
                     CQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAKTGRIWTYVRDDRNVGSSSP
                     PAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLFSAEREGGALTEVACWAHAR
                     RKIHDVYIRSKSATAEEALKRISELYAIEDEIRGLPESERLAVRQQRSKVLLTSLHEW
                     MVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRAVCLGKKNF
                     MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVL
                     TNK"
     gene            1213..1608
                     /locus_tag="ACHY5W_004802"
                     /pseudo
     CDS             1213..1608
                     /locus_tag="ACHY5W_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          543 a          485 c          586 g          493 t
ORIGIN      
        1 tttccagtgg cggtgagctg gattatctgg gggaagtcag cgctgaacag ctggaactgg
       61 tgagcagtgc cctgaaagtg atccgcacag aacgggtaaa aaaagcctgt acaaaatgtg
      121 actgtattgt tgaagcaccg gcgccgtccc gcccgataga gcgtggtatc gcgggccccg
      181 gattacttgc ccgcgtgtta acgggaaaat actgcgaaca tctgccactg tatcgtcaga
      241 gtgaaatctt tgcccgccag ggtgtcgaac tgagccgggc cttactctcc aactgggttg
      301 acgcgtgctg ccagttaatg acaccggtga atgatgccct gtaccgttat gtaatgaaca
      361 cccgcaaggt tcacactgat gacacaccgg taaaggtact ggcaccgggt cagaaaaagg
      421 cgaaaacagg gcgtatctgg acgtatgtcc gggatgatcg caatgtgggt tcgtcatctc
      481 ctccagcggt ctggttcgcg tactcgccga accggcaggg gaaacacccg gagcaacacc
      541 tccgcccctt ccggggtatc ctgcaggcgg atgcgttcac aggttacgac aggttgttca
      601 gtgcagaacg tgaaggtggt gcactgacag aagttgcgtg ctgggcccat gcccggcgaa
      661 aaatccacga tgtatacatc cgcagcaaaa gtgcgacggc agaagaagcc ctgaagcgaa
      721 tcagtgaact gtacgccatc gaggatgaaa tacggggatt accggagtca gagcgtcttg
      781 ccgtcaggca gcagcgaagc aaagtgttac tgacgtcgct gcatgaatgg atggtggaga
      841 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      901 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggat aataatgctg
      961 cggaaagagc gcttcgtgca gtctgtctcg gaaagaaaaa ctttatgttc tttggcagcg
     1021 atcacggcgg cgagcgtgga gcactgttgt acgggctgat cggcacctgc cgtctgaacg
     1081 gtatcgatcc ggaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
     1141 accgagttga tgaactcctg ccatggaacg tagtactcac caataaataa gcgtcaatac
     1201 ggtgctccgt tgacgcttac ggacgtcatg ctgggagcgg tggaacgccg cttcggcaac
     1261 gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta ccgggctaat
     1321 gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc ggtgcggagt
     1381 ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga ctacatcagt
     1441 gtcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc gttcgagcat
     1501 tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga atatctgcgg
     1561 cagcaagcca gtaatgggtt aagtgataac aggtgtctgg aaatataggg gcaaatccac
     1621 cgtttgatta tgcagtgatt aacaaaactc cgggaagatg aatgagtata atacctctcc
     1681 tgcaatccgt ttcctgagaa aacaggtggt aacacaatat tcttaatatg ttactgtttc
     1741 cccacacaac caggtttacg gttgtgtggg gaactatagt aatctcgggt aacaatccgg
     1801 agctgttcct tttatactct tcagactgtg tactcatcca cgccatccgt tttacagtct
     1861 cagtgtgtga ataataaatt acactttgaa gtgaacgcca gtcaggacca tgtcgtattt
     1921 ccgggtggat gagcatatat cagaaactac aataactaca gataacggtt ttcagtaatt
     1981 ctgtcataac ttgccggaat gtaatgtttt tgtgacatgg ttcttatttt ttatacacag
     2041 gcaggttttg tctggatgga atggataatg aactcttatc attataaggc ggtaacctct
     2101 tttttat
//
LOCUS       DAZUGK010000375         2067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZUGK010000375 DAZUGK010000000
VERSION     DAZUGK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.378"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..1369
                     /locus_tag="ACHY5W_004803"
     CDS             128..1369
                     /locus_tag="ACHY5W_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS4449522.1"
                     /translation="MNLQSGQNIPLQQSTIRLNLQYPAKSGFKGEPDTCLFMLNAQGK
                     VSGDSDFIFYNNLSSPEGAVRLVTGSQQASIEIALDRVPANVSKIAITVVIDGEDTIS
                     GLSLLSIQAPGIADFQAETQGRSEKAIILGEVYRHNGAWKLRALGQGFNGGLEPLAIN
                     YGVDVAQPAPQPAKPARISLEKKLETRSPRLVSLAKKASVSLTKNKLDTLEAAVAFVL
                     DASGSMSGQFSKGNVQSVLDRIAVLAAQFDDDGEMDVWGFGEKHKKYPNVTLDNLDTY
                     IQSIRGAGKRSSWENLPGLGGTNNEPPVMEEIVDYFKDSKIPVYVVFITDGGISKTRA
                     IKDAIRRSANYPIFWKFVGLGGSSYGILKNLDDFTDRRVDNTHFFAMDDFGSISDEKL
                     YDNLLEEFRPWIDETKRLGIL"
     gene            1527..1952
                     /locus_tag="ACHY5W_004804"
                     /pseudo
     CDS             1527..1952
                     /locus_tag="ACHY5W_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1949..2023
                     /locus_tag="ACHY5W_004805"
                     /pseudo
     CDS             1949..2023
                     /locus_tag="ACHY5W_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          561 a          468 c          516 g          522 t
ORIGIN      
        1 tcagcgttat gaaaaatggt gtcataccat atttacgcct caccgaatat ccagcgtcca
       61 gaactaagcc tagggaacat atataaacgc tgcgcattaa taataatcaa aaatcaggag
      121 agttaatatg aatttacaat ccggacaaaa catacccctt cagcaatcta cgatcaggct
      181 gaatcttcag taccctgcca aatccggctt taaaggcgaa cccgatacct gcctgttcat
      241 gcttaatgct cagggaaagg tcagtggcga ttctgacttt atcttttata ataatttgtc
      301 ttctcctgag ggggcagtca ggctcgttac cggttcgcag caggccagca ttgagatagc
      361 actggatcgt gttccggcga acgtcagtaa aattgcgatc accgttgtca ttgatggtga
      421 agataccatc agtgggctca gtttattgag cattcaggct cctggtatcg ctgatttcca
      481 ggctgagact cagggccgca gtgagaaagc aatcattctg ggtgaagtat atcggcacaa
      541 tggcgcctgg aagcttcgtg cgctagggca gggtttcaac ggtggacttg agcctttggc
      601 cattaactat ggcgttgatg tcgcacagcc agctccgcag ccagcaaagc ctgctcgtat
      661 cagtctggaa aagaaactgg aaacccgatc tccgcgcctt gtaagcctcg ctaaaaaagc
      721 ctcggtcagt ctcactaaaa ataaactgga cacccttgag gctgcggttg cgttcgtact
      781 tgacgcatcc ggctcgatga gtggccagtt cagtaagggt aacgttcagt ctgtgctgga
      841 ccgcatcgcc gttcttgccg cccagttcga cgacgacggt gaaatggatg tctggggatt
      901 tggagagaag cataagaagt atccaaacgt cactctggac aatctggaca cgtatattca
      961 gtcgattcga ggggctggta agcgttcatc ctgggagaac ctgccgggcc ttggggggac
     1021 aaacaacgag cctcctgtga tggaagaaat agtcgactac tttaaggact cgaaaatccc
     1081 ggtatatgtt gtatttatta ccgatggcgg gatcagcaag acccgggcga ttaaagatgc
     1141 aatccggcgt tctgccaact accccatctt ctggaagttt gtcggcctgg gtggttcaag
     1201 ctacggtatc ctcaaaaatc tggatgactt tactgaccgc cgggttgata acacccactt
     1261 ctttgccatg gatgatttcg gttcgattag cgatgaaaag ttatatgata atttactgga
     1321 agaattcaga ccgtggatcg atgaaacaaa aaggttaggc atcctttaat acatatagtt
     1381 agagcatggg tgaatgagct ctacagcagc ttaatactgt agagcctttc ttcattaaaa
     1441 ggtaaacgta aaccgaccgc catatgtagc cattagacaa gaattggtaa cttagacgcc
     1501 catctgacac agacggacat ctaagtatgg aattacagga ctggcgaaaa gaacctcgta
     1561 aaaagtattc gaatgaattc aaacttcgta tggttgaact ggcatcacaa cccggtgctt
     1621 gtgttgcaca gattgcacgt gaaaatggcg tcaatgataa tgttattttc aaatggctca
     1681 ggctctggca gaacgaaggg cgtgtttcgc ggtgtcttcc aataacgacc tcttctgaca
     1741 ctggcgttga attattacct gtagaaataa cgtcggatga gcagaaagaa cctgtggcgt
     1801 ccattacgcc gtctttaccc gcttccactt agaccagagt cagtgccagt tcctgcaagg
     1861 tggaattccg tcacggtaac atgatgctgg aaaatccatc gccagagctg ctcacggtgt
     1921 tgatccgcga actgagcggg aggggaagat gatttcactc ccatcaggta cccgtatctg
     1981 gctcgttgcc ggtgttaccg atatgtgtaa atctttcaac ggatacgaca tatgctgaag
     2041 gaggatctgt aatggattaa gtgataa
//
LOCUS       DAZUGK010000376         2050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZUGK010000376 DAZUGK010000000
VERSION     DAZUGK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.379"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..353
                     /locus_tag="ACHY5W_004806"
     CDS             147..353
                     /locus_tag="ACHY5W_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449523.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(609..881)
                     /locus_tag="ACHY5W_004807"
     CDS             complement(609..881)
                     /locus_tag="ACHY5W_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449524.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1041..1574
                     /locus_tag="ACHY5W_004808"
     CDS             1041..1574
                     /locus_tag="ACHY5W_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449525.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1795..1904
                     /locus_tag="ACHY5W_004809"
                     /pseudo
     CDS             1795..1904
                     /locus_tag="ACHY5W_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1906..>2050
                     /locus_tag="ACHY5W_004810"
     CDS             1906..>2050
                     /locus_tag="ACHY5W_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4449526.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELAN"
BASE COUNT          528 a          404 c          562 g          556 t
ORIGIN      
        1 gggccggaat gttatctggc atttttagca gagcctgaat gccataatca cggctcccgg
       61 agttggccgt cagtgggtga cactggcggc ttttttgttt ttctttactt tcattttctg
      121 tcggcggtga cggagacata catcagatgg aaaaaatcac aacaggtgtg tcatacacca
      181 cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc tctccgtccc
      241 agtgggtggc aataggtgtg ctgggaagtc tgctgtttgg cctgctgacg tatctgacta
      301 acctgtattt caaaatcaga gaggaccgtc gtaaggtggc gcggggagag tagtcgatga
      361 ataaacaata cgaactggtt gtaaaatgaa tatttctaac tgaaaaaacg ttccatgagg
      421 taagaaaagg tcacaggcaa tcaataacag gacgtgatga aagacccttg catttgtgcg
      481 ctttctcttt agatagcagc agatactgaa aatctgagtt gtcggggagt cagggataca
      541 gctgtgcaag agttggtcat tgtgattcca ttgaaatcct gtatgccatg aagggcagga
      601 ttttatggct acctgagctt tggtgatagt aagttgaaaa ttcgcatttt ttgctgacat
      661 gcgtaacgag aatcccataa acagggagga cttaattctt cattaaccca tgcgttgata
      721 ttatgtttca gccgttgaag catcagcggt gttaatgttg tggtaataat atccagcgtt
      781 ttatgtgaga tcttaccgta agggtctgca agaatgctgc ttgttgcttc gttattatct
      841 gccatcagaa gaagtaactc tgatttaacg ttttctgtca ttagttgtaa aaatcttctg
      901 cgcaaacttt ctttactgtt catttatatg gcttcatttg ttgtaatctg ctgcgtctca
      961 agggatatgt ttatgagagc gaccatgagt gtttgattat atacctaaca tatcaaggga
     1021 ttagaaatcg ataaatcccc atgaacgaaa aaataaaata cggcctgtcg gctgccgttc
     1081 tggcgctgat tggtgcaggt gcttctgcgc ctgaaatcct cgaccagttt ctggatgaaa
     1141 aggaaggtaa ccacaccacg gcataccgtg atggtgcggg gatctggacc atctgccgtg
     1201 gtgccacccg ggtggatggt aagcctgtta ttcctggcat gaagctgtca aaggaaaaat
     1261 gcgaccgggt taatgccatc gaacgtgaca aggcgctggc atgggtggag aaaaacatcc
     1321 gggtgccgct gaccgaaccc cagaaagcgg ggatcgcgtc attctgtccg tacaacattg
     1381 gccccggtaa gtgcttcccg tcgacgtttt ataaacgaat taatgcaggc gatcgaaaag
     1441 gtgcctgtga ggcgattcgc tggtggatta aggacggtgg cagagactgc cgtatccgtt
     1501 caaataattg ctacggtcag gtctcacggc gtgaccagga gagcgcgctg gcgtgctggg
     1561 acatcgacag atagcagaat attttcctga aaaatgacgt tggccaacgc gggtggataa
     1621 cacgaaatcc tgaaaactgg taaaacctaa gtgaataaaa gtaaaaaccc cgtttgttgg
     1681 cagcaagcgg ggttttgtgt tttctgacct tgagtaaggc aagggagaaa ttatgggtag
     1741 ggaggtactt tccctgtgag gaagtataaa agattctttc tgaggttgtc cattatgaaa
     1801 ggcattgaag tggagacgcc agtctggatt taacaagagc ggcagctttt gccattcgta
     1861 ttgtggccat tgctgttctg gtctgggcaa tccgttggtg gtgatatgaa ccgtgttctg
     1921 tgcgtggtta tcattgtcct gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat
     1981 taccgtgatc acgccatcat ctacaaagag cagcgcgata aaaaagccag tgagctggag
     2041 ctggcgaacg
//
LOCUS       DAZUGK010000377         1975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZUGK010000377 DAZUGK010000000
VERSION     DAZUGK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.380"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1323
                     /gene="escC"
                     /locus_tag="ACHY5W_004811"
     CDS             <1..1323
                     /gene="escC"
                     /locus_tag="ACHY5W_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFS4449527.1"
                     /translation="NEKPVKVLEKLSKLYHLTWYYDENILYIYKTNEISRSIITPTYL
                     DIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKA
                     KNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERSG
                     NLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEISVSIIDVDANDL
                     QQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNALQQNSKAKILSQ
                     PSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDSSNSLTGKR
                     RERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESLLLGGFIQDK
                     ESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            1337..1792
                     /gene="cesD"
                     /locus_tag="ACHY5W_004812"
     CDS             1337..1792
                     /gene="cesD"
                     /locus_tag="ACHY5W_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFS4449528.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
BASE COUNT          672 a          364 c          377 g          562 t
ORIGIN      
        1 aatgaaaaac ctgtaaaagt actggaaaaa ctgtccaaac tatatcatct cacatggtat
       61 tatgatgaga acatcttgta tatttataaa acaaatgaaa tatcaagaag tataattaca
      121 ccaacatatc tggatattga ctcactgcta aaatatctga gtgatacaat ctcagtaaac
      181 aaaaactctt gcaatgtgag aaaaattacg acatttaact cgattgaagt tcggggtgtt
      241 cctgaatgta taaaatatat tacatcttta tcagaaagtc ttgataaaga ggcacagtct
      301 aaagccaaaa ataaagatgt tgttaaggtc tttaaactga actatgcgtc agcgacagat
      361 ataacataca aataccgtga ccaaaatgtt gtcgtcccag gtgttgtctc cattctgaag
      421 acaatggcaa gtaatggttc acttcccagt accggtaaag gtgccgtcga gcgaagcggt
      481 aacctgtttg ataacagtgt aacgatttcc gctgatccac gtcttaatgc agttgttgta
      541 aaagaccgcg aaataacgat ggatatctat cagcaattaa tttctgagct tgatattgaa
      601 caaaggcaaa ttgaaatttc tgtctcaatt attgatgttg atgcaaacga tcttcagcag
      661 ctcggcgtaa actggtccgg tacgttaaat gcaggacaag gtacgattgc ctttaatagc
      721 tctaccgcac aggctaatat cagctcttct gtgataagta atgccagtaa ttttatgatt
      781 agggtgaatg cgttacaaca gaactcgaaa gctaaaatac tctctcagcc ttcaattata
      841 accttaaata atatgcaggc gattcttgat aaaaacgtaa cgttttatac aaaagtctcc
      901 ggagaaaagg tggcgtctct ggaaagtatt acaagcggaa cattgctgag agtgacacca
      961 cgtatacttg atgacagctc gaatagtctt accggaaaac gtcgggaacg agttcgtttg
     1021 ttacttgata ttcaggatgg caatcaatcg actaaccaaa gcaatgctca ggatgccagc
     1081 agcaccttac ctgaagtaca aaattcagaa atgacaacag aagcgacatt aagtgcagga
     1141 gaaagtttat tacttggcgg atttatccaa gataaagaaa gttcaagtaa agatggtata
     1201 ccgctgttaa gcgacattcc tgttattggt agtttattct ccagtacggt aaaacaaaaa
     1261 catagtgttg ttcgactctt tttaattaaa gcaaccccga taaaatctgc atctagcgaa
     1321 taataaatga ggctttatga gcaggaaatt tagctctcta gaggatattt atgatttcta
     1381 ccaggatggt ggcacattag cgtcattaac aaatctgaca caacaagatc tcaatgacct
     1441 tcattcttat gcctatacag catatcaatc tggtgatgtt ataaccgcaa gaaatctatt
     1501 ccatttgctc acatatctgg aacactggaa ttatgactac accttatctc tgggcttatg
     1561 tcatcagcgt ttatcaaatc atgaagatgc acaactgtgt ttcgcacgct gtgcaacttt
     1621 agttatgcaa gatcccaggg catcttatta ttctggaatt agctacttac tcgtcggaaa
     1681 taagaaaatg gccaagaaag cctttaaggc ttgtttaatg tggtgtaatg aaaaagaaaa
     1741 atacactaca tataaagaaa atattaaaaa attgttaggt aatacagagt aaaaactacc
     1801 atcagctata ccaatcccca ggctgactgg ggattttcta tcggaaataa taaagctgct
     1861 atagtgaagt gctcccaaag ttgagcaccg accactggta gatttatcta caggtgtggg
     1921 gttattccat taaccaggag tttatgccct gaactatctt ctgctgtatt taata
//
LOCUS       DAZUGK010000378         1962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZUGK010000378 DAZUGK010000000
VERSION     DAZUGK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.381"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..938
                     /locus_tag="ACHY5W_004813"
     CDS             33..938
                     /locus_tag="ACHY5W_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFS4449529.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            935..>1962
                     /locus_tag="ACHY5W_004814"
     CDS             935..>1962
                     /locus_tag="ACHY5W_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4449530.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITE"
BASE COUNT          455 a          479 c          593 g          435 t
ORIGIN      
        1 aaggcgctga gggaagagct ggagacaatg ctgtgacttc accatttatt caacaaattg
       61 ctgataaccg ggtatgtcag gtactcacct gtcttcctga aaaatttgtg gttgatttcg
      121 caaacggaat cgatgttgca caggagcata tccgcacggc cggagagcgc acgttcttcc
      181 ggcgtttaaa agaggggctg accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt
      241 cgcttgcaca gggcgttgag gcgtccctgc gctggctgac ggagatgaca acgtcacttg
      301 ccaccacaaa ctacgcgatt acccgggtaa acgacagggt cagttcactg gtcagtgata
      361 cagccaggct ggcgcattat tccgcagata cgcgggagca gttactcacc ctggccgatc
      421 aggttcacca taaactgaat catcttgaag aaaaactcca ccgtgttgac caggttcagc
      481 gggcgcagtt acatcttgag cagatattct catggtggag cgccgggcga tatgcgtcat
      541 tttcccctgc cggacgttgt tatgtggcac ttgaagagct tcgctggggc gcgtttggtg
      601 atgtgatacg tcagggcgaa acaggccagg ttaaccagct actggatatt ctcagacata
      661 aagcattaac gcagatggca caggagagtg gcggtagtgc aaccgtgcgt ctgaacactc
      721 tggactggct tggtggtcag ggccgggagc aggcagataa cgagtggcat gacgcgatta
      781 actggctggg agactggtgc agtgaagagc agcatccggt gatctggtca accacgcagg
      841 ctgcagaaca cttaccggtt cgcatgcctc gcctctgctc tgcagaacgc ctctctgaaa
      901 gtatggtcga tgaaatattt cagaaggggg ctgcatgagt acagaaatga aaacggggct
      961 ggtgctgtcc ggtggcgggg cggtgggcgc ttatcaggcg ggagtggtta aggcactggc
     1021 agagtgtggt acacagatca gcatggtttc agggaccagc attggcgcat tcaatggtgc
     1081 cattatcgcg gcctctcccg atctgtcaga agctgccgta cgcctggagg cgctctggga
     1141 gcatctgggg aataatcagg tgctgtcggt aaacagattg gtttactttt cattgctgaa
     1201 aaaattgttc caggcaatga acctctgcca gatccccgga cgtgcaggag cactgcttac
     1261 gacgcttctt cgccatatat cgacaatcaa cgggtttgac aatctgatgg ctcagccgtt
     1321 gttgtcagat gagcccctga cagcgctgat ggatcattat cttgatactg atgctctggc
     1381 agacgggcta ccgctgtatg tgtcgctgta ccccacagaa gggggcatgc aggatattat
     1441 tgactgcatt cgtgctgaac tgggtgtcgg aaccacgaaa aacgccgttt ttcagcatat
     1501 ccagagcctg ccccgcggac agcagaaaga ggctctgctt gcgtcagccg cgctgcccct
     1561 gctgttccgt ccccgtgagg ttcaggggac aatgttcggt gatggtggta tgggaggatg
     1621 gcgaaatatg cagggaaata cccctgtgac gcctctggtc gatgccggat gcaatatggt
     1681 gattgtgacg catctgagtg acggttcttt atgggatcgc caggcttttc cggacaccac
     1741 aatccttgag atccgtcccc ggaaaaggct gaaatatgca ggtgatggtg gcaacagcgg
     1801 cggtctgctc agttttacat cggcacatac cgacgcctgg cgtcagcagg gctatgaaga
     1861 cacgatgctg gcgatggagc atatccggaa accgctggca gcacgtcagg cactgacccg
     1921 gtcagaggcg gtattgcaga aaagcctgga tataacggaa ga
//
LOCUS       DAZUGK010000379         1957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZUGK010000379 DAZUGK010000000
VERSION     DAZUGK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.382"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..349
                     /locus_tag="ACHY5W_004815"
     CDS             <1..349
                     /locus_tag="ACHY5W_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="HFS4449531.1"
                     /translation="KSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVV
                     VWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHD
                     KVIGHYLNIKHYQ"
     gene            366..626
                     /locus_tag="ACHY5W_004816"
                     /pseudo
     CDS             366..626
                     /locus_tag="ACHY5W_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(768..1892)
                     /locus_tag="ACHY5W_004817"
     CDS             complement(768..1892)
                     /locus_tag="ACHY5W_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFS4449532.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          568 a          445 c          396 g          548 t
ORIGIN      
        1 taaatcacgt cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc
       61 gcacgtcttc ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc
      121 ctttgaggtc gtggtatgga tgacggatgg ctggccgctg tatgaatcac gcctgaaggg
      181 aaagctgcac gttatcagca agcgttacac tcagcgcatt gagcgacata atctgaatct
      241 gagacaacat ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct
      301 gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat
      361 tacctgagaa taatacctcc tgggtaagtg agcagatact ttcagcgcct ccgaaagatg
      421 taaatattcg acttggagtc ggttctttag aaggtacaac cgtctcacat gttcagcgat
      481 tgcatcattc gttaatcgca gctggtttgg aaagtaacct cactgtctat accggtggtc
      541 acgattatgc ctggtggcgc ggagcaatta ttgatgaatt agcaaattat aattgcagga
      601 aggtatcaaa taataactct gtgtaaaata tcgttttatc gtcagataac tctttctgca
      661 atattattat gggagactat ctcatttatt tagtgatgtc cttcatttat gtgatgaagg
      721 acattatatt actcataaca tttctcgttg aaaatttcag caatacgcta caattgtttc
      781 ccgttgtttt ttaattttct catcaaaacc tctgcaatct ctgcaggact gggttgttca
      841 gccatttcag cggctacctg atctgaacag aagcgtagtg aatcatcata aagaaggcga
      901 tttaccaaat cactggtcag tccatgcttg tccggaataa tcccacaccc acgcttcgca
      961 acaatttctg cattaacaga gcgatctgca ccttcgccaa acacaatctg tggtattcca
     1021 ctatacagcg ctgtcagggt attacctgcg ccaccgtgat gaataaatcc atcagctcca
     1081 ttaaggaata cacccatagg tatccagtca accagacgta catttgatgg tagttttcgt
     1141 aaaccagtac gagcatttat tgcaagttgc aaaatgatat cagcatcaac ttcatttgca
     1201 gaatccataa cccatgaaat cagctccaga ccatcaacca ttggttttac agtgccaagg
     1261 ctgataagta aacgtttccg atcagaatcc ctgtcccacc atgtttcctt tacagcacct
     1321 ccgttataag gaatatatct cattgagata accggttctc cagcattttt aagaatgctc
     1381 atacttgggg gagcaacatc aatccatgct aaatcacata gtggaccgct gactccatgc
     1441 cgcctgtaag cattgcttaa agaacggttt aacatctgaa tatgggcaga tgtatgcgcg
     1501 aatccaacag ccagcatcac tgaaggaatt ctatatttag cagcaaccaa tgggcctgcc
     1561 ggatcaagag ggggatagac tattaaatct ggcctccatt ttcctgcaaa atcgatgagg
     1621 ttatctgcca tttcatcgct aaaaaaagag aaatgaccaa taatattact ttttttcctc
     1681 aactcttcct gatggcgata tccagcctct gaatctaaac ctggaactgc atcaaaaaca
     1741 accagtccag cttctgctgc tttattcgcg aatttgccag cgctactaat tactacatca
     1801 tgtccaatta cacgaaaggc ctgagccaga gaaatcaatg ggtataacaa accgtacagc
     1861 ggtgggccaa taaaaagaat acgttttcgc attaaaaaag tccgtcttct gcttacctgt
     1921 ttatttttct atctctaaga aaacataatt aatatta
//
LOCUS       DAZUGK010000380         1953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZUGK010000380 DAZUGK010000000
VERSION     DAZUGK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.383"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHY5W_004818"
     CDS             <1..276
                     /locus_tag="ACHY5W_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4449533.1"
                     /translation="LQELCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGM
                     LTNLNHAAMSTLLGERIMDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            298..1014
                     /locus_tag="ACHY5W_004819"
     CDS             298..1014
                     /locus_tag="ACHY5W_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4449534.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            1047..1328
                     /locus_tag="ACHY5W_004820"
     CDS             1047..1328
                     /locus_tag="ACHY5W_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4449535.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1325..1552
                     /locus_tag="ACHY5W_004821"
     CDS             1325..1552
                     /locus_tag="ACHY5W_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449536.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1545..1856
                     /locus_tag="ACHY5W_004822"
     CDS             1545..1856
                     /locus_tag="ACHY5W_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449537.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          530 a          388 c          596 g          439 t
ORIGIN      
        1 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
       61 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
      121 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gcacgcttct tggtgagagg
      181 attatggacc gcatgaccat gaacggtggt cggtgggtga cgtttaactg ggatagctgg
      241 cgtccaaatg tcagcaatca gagggttgtg aagtaatttt tgttggagga cgttttaatg
      301 gaaactgtat ttgacgcact gaaagcactg aaaaaagcct cttcacaggt agtggcatcg
      361 cgccttggaa tcagccgcga agatgctgtc aacgaactgt ggaaactgaa gcgccgtggt
      421 gaagcggata acaagggttc gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg
      481 tcaccggtac cgaaagtgac agcgcaaatg ctgactgagg cgattgaaca tcatggccca
      541 caaacggcgg atgagctggc actgatgttc gggattacct cccgccgggc gaattcatca
      601 ctggccatgg caatcagcaa agggcgtctg attcgcgtga atcagggcgg taaatttcgg
      661 tactgcatac cgggcgctga tttaccggca gagccgaaag ccgcatccat agcggaaacg
      721 gatggtaaag cctttcctca gccagcaggt gttgcgttac cagtcgggga agcggaaaca
      781 caggaagaaa taaaaacgga aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc
      841 agaaagcatc cggatggtct gattttacca tcgctgcatg tggctaaccg cgagctgcgc
      901 cgggcaaaag gtcaggttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
      961 aagtgccggg atattctccg ggatattacc gccaccagag aacagcagcg gtgagtgggt
     1021 ggaagacgtg gtgccgggcg gaaatcatga tactccggca gtgtgcggga acgaggaagg
     1081 taaaaagcgt tggcgcactt atcggacgaa ctgaagcggc agtgagaacg aaggcacggg
     1141 agctgggcat cagcatgatg ttacgtggtg attttcaccc gtcggcaaaa tattctcagc
     1201 gtgatattga gctggcgcgg caactgcatc agagaggcat gcaaagaagg gaaattgcca
     1261 gaaaattagg catgccgctg cgcatagtga ataactacgt ttatttcgac aggagggtgt
     1321 ctgcgtgaaa atcctgtatc aggattacgg cccggtgggg caggtggtta tcagcagtac
     1381 tgtaatggag tttcggaagc ataaccgtgt ggtggatgct gtgctgttaa cctgtccggg
     1441 gatatcggcg agtcgtgcag gtgtgtttat tatgaagacg aaattatatg gcagtaaggc
     1501 gtggataaag aaggcgtatc gtgtagcgtt gcaggaggtt aacagtgagt aaaattaaag
     1561 aaatgccggt agttcgtgac ggatatggct actggacaca tcctgaatat gaaaaattct
     1621 gtgatggtcg ggaatatatt tcaacggaag agtttaacgc ctggatggag gaaaataatc
     1681 ttcaatacgt cctctgcttc agagatgaag gatgtgctga ccttgatgcg tgtgatgctg
     1741 atatttctgc atgggaaccg gaacgaccag agggcgatgg ctggtttatt ggttccattc
     1801 atgatacgga agatggcccg gtttgtgtct ggttgcgaaa taaggctgaa gcataaaggc
     1861 gataaaccaa ctaacaacta aatactgaag atttaaatca gaaacgattt ttattaaatc
     1921 cttaaccgga gggattcctg caccctcaga aca
//
LOCUS       DAZUGK010000381         1909 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZUGK010000381 DAZUGK010000000
VERSION     DAZUGK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.384"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1909
                     /locus_tag="ACHY5W_004823"
                     /pseudo
     CDS             1..1909
                     /locus_tag="ACHY5W_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          665 a          383 c          355 g          506 t
ORIGIN      
        1 atatcaactt ttcacgcaga aaaaatcatt aatgatctgt taaaaaacga ttataaaatt
       61 tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagcg tagcattaca
      121 gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac aatggttgaa
      181 agaggtgata acagcggtgt tcatcagtat ggtcctgctg aacatttcac ccacattata
      241 tctgacaaac cctccccaaa agataaatat gttgcatatg ctattaacat tcctgactat
      301 gagctggcag ccgatgtata taatattaac gtgacgtcac cttccggaca gcaagaaaca
      361 tttaaaatat taatcaatcc agaacatcta cggcaaacac ttgagcgtaa atctcttact
      421 gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga agcgattctt
      481 catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt tgcacgttgc
      541 cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg accagaaact
      601 cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct ttgtaccatc
      661 cataatccaa tggggatttg caggatttta tcgcattggt taaagatgct ataaaatttg
      721 gtatcgatat cattcctgat tataccttta actttatggg aattgggggt agtggtaaaa
      781 atgacctgga ttatccctct gctgatatac gggcgaagat cagtaaagat atagaaagtg
      841 gtatccctgg ctattggcaa ggtcaggttt tgattccatt tactatagat ccagtaacaa
      901 aagaacataa acaaatccat ccagaagata tacatctcac tgcaaaagac tttgaaacaa
      961 gtaaagataa catctctaag gatgaatggg aaaacctcca tgcattaaaa gaaaagcgtt
     1021 taaatggaat gcctaaaaca ccccccaaaa gtgaccaggt tattatgctg caaaatcaat
     1081 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttatgatgca gcaaaacact
     1141 caaaacatga acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcgat
     1201 tacacaatac taacttattt aacccaaaat atcatgaaaa agccgttatg aattacatgg
     1261 aatatctggt aacttgccag ttggatgaac aacaaatgtc atcgctactt tatgaaagag
     1321 atgatttaag cgccattgat ttttcattgc tcatgaagac gataaaagcc ttttcatttg
     1381 gtggcgatct ccaaaccctt gcatcaaaac cgggttccac aatctcaagc atcccatcag
     1441 aaagacggat attgattaac attaaccacg attttcctaa caatggcaat cttttcaatg
     1501 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
     1561 cgttcagcag gcctttagtt tactgggatg gccaagtatt aaaatcaacg actaagatta
     1621 aaaatgatga tgggtccacg cgtgtcggcg gtgaggcgtg gcttaataaa ggttgctcta
     1681 cctatcagca gctctacaat gaattccacg cattatatat agataaagca ggaatatgga
     1741 gcgcatttga gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattctg
     1801 tgaatattaa tcactctcct catgacggac tagttgtaat aaataaagga aacgaagaaa
     1861 ttgaaggtgc ctggcctaac aaattgcaac caggaatata taaaaatat
//
LOCUS       DAZUGK010000382         1900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZUGK010000382 DAZUGK010000000
VERSION     DAZUGK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.385"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..424
                     /locus_tag="ACHY5W_004824"
     CDS             182..424
                     /locus_tag="ACHY5W_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449538.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGARCGLDDVFPPDGKHSENSIGAVDS"
     gene            421..843
                     /locus_tag="ACHY5W_004825"
     CDS             421..843
                     /locus_tag="ACHY5W_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449539.1"
                     /translation="MKIRHEHIESVLLALAVEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDEAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            921..1709
                     /locus_tag="ACHY5W_004826"
     CDS             921..1709
                     /locus_tag="ACHY5W_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449540.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNANCNARGVTKSAIDRITFMAGFADALIQVGWLVENDVGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAAIVTQTDQKALPEEEEEEDINTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            1716..>1900
                     /locus_tag="ACHY5W_004827"
     CDS             1716..>1900
                     /locus_tag="ACHY5W_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4449541.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRS"
BASE COUNT          532 a          426 c          501 g          441 t
ORIGIN      
        1 caagcttctg cctgcgctct ttaagacgca ttgcaatttt gtcattgagc ctactagcgg
       61 caattttttc gttttctttt tccattgcat ccttgtatca cgaatcgtga tttacataaa
      121 acacaaaaca gcttgaccat ataacacaag gtgtgtttaa aattgtcatc ggaggttttc
      181 aatgaacaaa atttcaacat atcgaaaaca gcttgggctg tctcaaagac aacttgctgt
      241 tcagttaggg tggatacaaa gccgactggc aaattacgaa gcaaattttc gtacccctgg
      301 actagaggag tgcagaaaaa ttgtttctac ccttaatcgg cttggcgctc gttgtggact
      361 tgacgatgta ttccccccag acggtaagca tagcgaaaac agcataggag cggttgattc
      421 atgaaaatca ggcatgagca tatcgaatca gtgttgttag ccctggctgt tgaaaaaggg
      481 caggcatggg tagccaatgc cattactgaa gaatatctgc gccagggggg cggcgaattg
      541 ccccttgtac caggcaagga ctggaacaat cagcagaata tctatcaccg ttggttgaaa
      601 ggtgaaacga aagcgcaaag ggaaaaaatt cagaaactga tccctgcggt tctggcaatt
      661 cttccgcgcg agctgcgtca ccgactctgc atcttcgata ccctggaacg ccgtgcatta
      721 ctggcggcgc aggaagcgtt gagtacggca attgatgcgc atgatgaggc agtccaggcc
      781 gtttaccgga aagcacattt cagcggtggt gggtcgcccg gcgattctgt cgtagtgcat
      841 tgattgaaat taatcgtgcc ggattgtttt gttcggtatc agttaaatgt aacgctgcga
      901 gcgttacaag gtgaaaacaa atggcttcaa actggataaa gctcgaggtt attacgccgg
      961 ataagccgga aatattcagg cttgctgaga ttctgaatat tgatccagat gccgcattag
     1021 ggaaggttat tcgcttctgg gcatgggcgg atcaacaaat gatagacggt aatgcaaatt
     1081 gtaacgctcg cggcgttaca aaaagtgcaa tagatcgcat cacttttatg gctggttttg
     1141 ctgatgcgtt aattcaggtt ggatggctgg tcgaaaatga cgttgggctt tctctaccta
     1201 actttgaacg tcataacgga aaaagctcta aaaaacgggc ggttacaaac gagcgagtta
     1261 caaaaatacg cgaactgaaa cgaaaaggta acgctgccat cgttacacaa acggatcaaa
     1321 aagcgttacc agaggaagag gaagaggaag atataaatac tgatctcccc ctaaatcccc
     1381 ctcgccaaaa acgagcgtct aaaaaattcg agccggaggc tattgagctg cccgattggt
     1441 tgccggaaac actctggcat gagtgggtcc ggttcagaca ggcattgcga aaaccgattc
     1501 gaacggagca gggcgctaac ggggcgatac gggaactgga aaaattccgc cagcagggtt
     1561 ttacacctga gcaggtgatt cgacacagca tcgccaatga ataccagggc ctgttcgcgc
     1621 cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca
     1681 gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg acaggcggcg
     1741 ttctggaacg catccgccga ctgaccccgc cacatgtaac cgccccattc agaacggtag
     1801 cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag attaaccgcc
     1861 tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgttc
//
LOCUS       DAZUGK010000383         1874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZUGK010000383 DAZUGK010000000
VERSION     DAZUGK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.386"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..187
                     /locus_tag="ACHY5W_004828"
     CDS             <1..187
                     /locus_tag="ACHY5W_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFS4449542.1"
                     /translation="KCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            458..1027
                     /locus_tag="ACHY5W_004829"
     CDS             458..1027
                     /locus_tag="ACHY5W_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449543.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            1027..1173
                     /locus_tag="ACHY5W_004830"
     CDS             1027..1173
                     /locus_tag="ACHY5W_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449544.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            1401..1586
                     /locus_tag="ACHY5W_004831"
     CDS             1401..1586
                     /locus_tag="ACHY5W_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS4449545.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          493 a          422 c          517 g          442 t
ORIGIN      
        1 taagtgtttc ccgtcgacgt tttacagacg gattaatgca ggagatcgaa aaggtgcctg
       61 cgaagctatt cgctggtgga ttaaggacgg tggcagggac tgccgtattc gctcaaataa
      121 ctgttacggt caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct ggggaatcga
      181 cagataagca gaatattttg ctgaaaaatg aggtttgctt acatggatgg ataacacgaa
      241 atcctgcaaa ttggcaaaat gtaagtgaat aaagtcaaaa cagttgttta acactcaggc
      301 accgtaatga tgcctttgtc atttctgcgc atctcacgcg catctcacaa cacagaacct
      361 ttcaggatga cccttgagga taccggtttg gctgtcggtg cctttctgtg ggctggattc
      421 ctgtgagaca aggttcatca ctaaaaggaa ataaccgatg aatatgatgg ccgtgccgtt
      481 tcacggcaac tctctttatg tagttaacca taatggcgaa ccatacgttc ccatgaaacc
      541 tgtcgttgcg gggatggggc tggcctggca atcacagttg gctaagttaa gacagcgttt
      601 tgcgtcaact ataacggaaa tcgttatggt tgctgaggat gggaaacaac gcaatatggt
      661 gtccatgcca cttcgaaaac ttgccggctg gctacaaacc attaatccca acaaagtaaa
      721 acccgaaatc cgcgataagg tcatccggta tcaggaagag tgcgacgatg ttctttacga
      781 gtactggacg aagggttttg tcgttaatcc ccgtaaaatg agcgtgatgg aagaactcaa
      841 ccaggcttgt gctgacatga aacgggataa aaacattgcc agtgtgtttg ctaccgggct
      901 gaatgagtgg aaacaggtta aagccgcgca tgtatcaaaa atccgtacgc tggtaaatga
      961 agcgaatatg ctgattgatt ttgtcctggc tgatacaggc aaagggaaaa taacaaaggc
     1021 ggattgatgg ggtggctaat gatatcagat aaactcataa cgctggtgaa gagcctctgt
     1081 gtacttgtcg gcatttcatt tttagtcatg ctggttgcca ttttcttttc caccgcctgg
     1141 cgagtcctga cgttatcggg actggtgggg tgaaagagag atgaaccgtg ttctgtgtgt
     1201 ggtgattatt gtcctggcgg ttggctgtgg tgcgctgtgg ctggcaacaa accattaccg
     1261 tgacaacgcg ctcacctaca aagcgcagcg cgataaaaaa gccagagagc tggaacaggc
     1321 gaatgccacc attactgaca tgcaggtgcg ccagcgtgat gttgctgcgc tcgatgcaaa
     1381 atactcgagg gagttagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc
     1441 cgctggtcgt aagcgcctgc ggatcaacgc cacctgctcc ggtaccgtgc gtgaagccac
     1501 cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacaccg ctgaacggga
     1561 ttatttcatc ctcagagaac ggttgatgac aatgcagaag cagctggaag gggtacagga
     1621 ctatatccgc actcagtgcc tgaactaagt tttgctgatg cgccgtatcg tcgccgtatt
     1681 cctgcattaa cagagaccgc agcccgacag ggagactcct ctgcgagagt gtgcggggat
     1741 aatcaaaaac gatacacacc ggggtttacc gcgtaaacgg agcgcggcgt tctcccctca
     1801 tggtcgcccg tccggtgcga tggtggaaga aactggaatc tgttcaataa aaaaactgcc
     1861 gtgttggagt caca
//
LOCUS       DAZUGK010000384         1865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZUGK010000384 DAZUGK010000000
VERSION     DAZUGK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.387"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1865
                     /gene="eae"
                     /locus_tag="ACHY5W_004832"
     CDS             <1..>1865
                     /gene="eae"
                     /locus_tag="ACHY5W_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFS4449546.1"
                     /translation="LLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYS
                     SESEMMKAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTK
                     SNMTDDKALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGT
                     AEVNLQSGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPAN
                     MLGYNVFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPAN
                     GFDIRFNGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVT
                     MGIDYRHGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNI
                     ILEYKKQDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHS
                     GSQSAQDYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGV
                     TDFTADKTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANG
                     KATVTLKSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVAN"
BASE COUNT          512 a          385 c          450 g          518 t
ORIGIN      
        1 aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa actggtgaaa
       61 ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg tcgttgaata
      121 agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag cagatcattt
      181 tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt tcggcacctc
      241 ttgttgctgc aggtggtgtt gctggtcaca cgaataaact gactaaaatg tccccggacg
      301 tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa caggcggcga
      361 gtctcggtag ccagcttcag tcgcgatctc tgaacggtga ttacgcgaaa gataccgctc
      421 ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa cattatggga
      481 cggcagaggt taatctgcag agtggtaata actttgacgg tagttcactg gacttcttat
      541 taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg cgttacattg
      601 actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct gcaaacatgt
      661 tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt ttaggtattg
      721 gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc cgcatgagcg
      781 gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat ggcttcgata
      841 tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg atatatgagc
      901 agtattatgg tgataatgtt gctttgttta attctgataa gctgcagtcg aatcctggtg
      961 cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg atcgattacc
     1021 gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt tatcagtttg
     1081 ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga acattatcag
     1141 gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac aagaagcagg
     1201 atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt acgcagaaga
     1261 ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat gatagtgcat
     1321 tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa gactaccagg
     1381 ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg gctcgcgcct
     1441 atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt ctgtcgaatg
     1501 gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact tcggctaaag
     1561 cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg gtagctcagg
     1621 ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg gcaaatagtg
     1681 ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg ccaggacagg
     1741 tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt gcggttatat
     1801 tttttgatca aaccaaggcc agcattactg agattaaggc tgataagaca actgcagtag
     1861 caaat
//
LOCUS       DAZUGK010000385         1863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZUGK010000385 DAZUGK010000000
VERSION     DAZUGK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.388"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..911
                     /locus_tag="ACHY5W_004833"
     CDS             27..911
                     /locus_tag="ACHY5W_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449547.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            1154..1210
                     /locus_tag="ACHY5W_004834"
     CDS             1154..1210
                     /locus_tag="ACHY5W_004834"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449548.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(1613..1861)
                     /locus_tag="ACHY5W_004835"
     CDS             complement(1613..1861)
                     /locus_tag="ACHY5W_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449549.1"
                     /translation="MVRRISMNTICSLIVVVALSLLSFSSVAKTITAVGSTIDSTEKE
                     ISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
BASE COUNT          503 a          419 c          434 g          507 t
ORIGIN      
        1 tgccggcatg aaggggggat gtttcaatgg cgaaaaggga gaactacaac gagctgtacc
       61 tgtttatgca ggtggtgcgg gaaggcagct ttaccgcagc ggctaaacgg ctgggtcttg
      121 cgcagtcagg agtcagccgt tctgttcgcg agcttgaaga aaggctgggt gtccagctgc
      181 tagtgcgcac cacgcgtaaa ctgtcgctga cacaagcagg cgagcagctc taccagaaga
      241 cggcatctgg atttgaaatg ttagatcttg ggcttgccac actggcgcac tatcgagaga
      301 cgccctccgg cacggtgcgt atcaatgcca gccagcacgc aattgataaa tgcctgctgc
      361 caaagctggc ggtatttaaa cagcgatatc ctgatatccg gcttgaactg ataaatgaga
      421 gccggttcgt cgatatcatc gaggaaagat tcgatgcagg tgtccgcctg gggcctgaag
      481 tcggccaggg tatggtcgcg gtacgcatca cgcccgatat ggagatggca attgtgggga
      541 ctccggaaca ttttcgtcgc tatggttttc cgcaaactcc ggcggattta aaggcgcatc
      601 cctgtatcgc ctatcagttt gccgacggca gcgtatacca gtgggaactc aatcaggatg
      661 ataaaaaaat cactcatcgc cctgaagggc aatgggccct atccgacagc tatatggaag
      721 cagaagccgc ccggctgggc ctcggattgg cctatgttcc tgttgaactg gtcgcggatg
      781 atttagaaca cggtaagctt atcagggtgt tgcagcgtta cagcctgcga atggagggat
      841 tgtttctcta ttaccctcat cgcaacgtct cccccgccct gcggatggtc attgatacat
      901 tgaaaatctg aacgttaata acgaccgact cttcactgtg caaaattgtg tcaggtagtc
      961 cctctgcgtt caattccagg gtctgctttg agcgagaaag atcagactta tctgtacaag
     1021 gggactatcc agcaatggag atcatccaaa agaaaaaacc acctggctcc cctcgacaac
     1081 cttaatccgg gcatttagcc tcaatcaaca ttttcatatc tgtcaggtta cttcattatg
     1141 aaaaccgatc aatatcgttg tttttgttgc aacagtagaa tacgcgtatt tatccctcca
     1201 tttctattaa taacggcaat aaactgttca tttcactgat atttaaaata tgcatccttt
     1261 tcaagctgtt cagcggcata agaaaacgta ttaaatatga attcgacgat ttaaccattt
     1321 ctgtctgatt ggtaatcatc tgtgattaat gctggtctat tgattacgcc agcattaacc
     1381 gcttagggat gtattcttcc tgttgatata cagccctcga aaaagagaga aaataaaact
     1441 gtgtccaata ttactttact actccagacc gaagcatcgg tagaattagc agaacgactg
     1501 gtacagaagg tgggtatagc gtcatttgtc gggcattcca ttgccatcat aatgataatt
     1561 ttgaatcaat agacgtgctt cttgatcatg tcttaaaatt ataccatgag ccttatgaag
     1621 catcattttt actgactggt gttaactttg ctatcatata aactctgttc ttaaaaaacg
     1681 cgcccagaat tttatatgat ttcccttgtt tttctgcctg taaagaaatt tctttttcag
     1741 tgctgtcaat ggttgaaccg acggcagtaa tcgttttggc gacgctactg aatgataata
     1801 aagacagtgc aaccacaaca attaaagaac agatagtatt catgctaatt ctcctgacaa
     1861 ttg
//
LOCUS       DAZUGK010000386         1851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZUGK010000386 DAZUGK010000000
VERSION     DAZUGK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.389"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(85..660)
                     /locus_tag="ACHY5W_004836"
     CDS             complement(85..660)
                     /locus_tag="ACHY5W_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS4449550.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(729..1307)
                     /gene="terD"
                     /locus_tag="ACHY5W_004837"
     CDS             complement(729..1307)
                     /gene="terD"
                     /locus_tag="ACHY5W_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFS4449551.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1356..>1851)
                     /gene="terC"
                     /locus_tag="ACHY5W_004838"
     CDS             complement(1356..>1851)
                     /gene="terC"
                     /locus_tag="ACHY5W_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFS4449552.1"
                     /translation="LRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALYATPLFLCV
                     AVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALKQYLVHLEK
                     AVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVMFPGKPESE
                     EKGS"
BASE COUNT          462 a          502 c          447 g          440 t
ORIGIN      
        1 cccccctttc ccatattcac ccgttgatag cggatcgata cccaaaaaaa accccggcat
       61 tgccggggtc agactcagcg tcttttagac attgacgccg tgctgggaag caagcgctga
      121 cagaccaccg gcaaaaccct ggccaacagc tttaaacttc cactcagtac catggcgata
      181 cagttcaccg aagaccattg cggtttcggt tgaggcgtct tcagacagat cgaaacgggc
      241 aatttccgtc ccgttgtcgt tgttgtaaac gcgcatgaag ctgttgctca ccatgccgaa
      301 gttttgttta cgcgcttctg catcatagat ggtaacggca aataccagtt ttttgatgtc
      361 tgctgagact ttggtcagat cgattttgac ctgctcatcg tcgccgtcgc cttcaccggt
      421 acggttgtcg ccctggtgct ctactgcgcc atcagggctg gttttattat tgaagaaaat
      481 gaaatgggca tctgacagta ctttaccgtc ttcacctact gcgaatacgg aagcgtccag
      541 atcaaaaccc tgaccatcgg ttacacgggc atcccagccc aggccaacca tagcgacatt
      601 catggttggt gcttctttgg tcagagatac gttgccgcct tttacgagag aaactgccat
      661 ttttagctcc tgcaaacagt tgaatgaggc tgaattaaca gccccagata tgaaaagtta
      721 cttttcgatc aggacgcgtt aatgccgtac tgagcacata cagatgccag accaccagca
      781 taaccctggc ctactgcgcg gaatttccac tcaccattgt ggcgatacag ctcgccgaac
      841 agcatggcag tctcagtgga cgcatcttcg gtcagatcgt agcgagcgac ttcagtctgg
      901 ttatcgtcat taaccagacg aataaacgca ccggatacct gaccaaagct ctggcgacga
      961 gcctgagcat cgtggatggt cacaacgaag atgatcttgt caacttcaga cgggacggcg
     1021 tccagtttaa ttttcagcga ttcatcatca ccatcgccct caccggtgcg gttatcgccg
     1081 gtgtgcgtta cggaaccgtc ggatgacgtc aggttgttat agaagatgaa atctgaatcg
     1141 ccgcgcactt tgccgtttga ggccagcagg aatgctgaag catccaggtc aaagtcctga
     1201 ccgtctgttg aacgcgcatc ccagccaagg cccaccagga catttttcat tgacggagct
     1261 gctttactca gggagacgtt cccgcctttg gaaagagaaa cactcataaa ataacctctt
     1321 cgattagtaa ttgttcaggt taacacttaa ggggattaac tccccttttc ctcagattca
     1381 ggtttgcccg ggaacatgac gcttgcgaga atgcccagcg ccagtacacc cagtacaaca
     1441 tacaggctgg ttgttgccgc gatgctgtaa ccatgatgcc agatgtgatc ggtcgcattc
     1501 aggccgagtt ttgccgcgat gaagaacagc agcacgataa cggccttctc cagatgaacc
     1561 aggtactgtt tcagtgcctc aaggacaaaa tacagagtac gcaggcccag gatagcaaac
     1621 atcatggcac tatagacgat aagcggttca cgactgacgg caatgattgc cggtaccgag
     1681 tcaaacgcga acattacgtc cgagagttca accacagcca cacacaggaa cagcggggtc
     1741 gcatacagcg ccgctttttt accacggcca atggtgacat cgctgttttc tggtttcgcc
     1801 agttcagcat ccacttcctt ctggttaagt aggaaggcat gccctctgag c
//
LOCUS       DAZUGK010000387         1840 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZUGK010000387 DAZUGK010000000
VERSION     DAZUGK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.390"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..758)
                     /locus_tag="ACHY5W_004839"
     CDS             complement(<1..758)
                     /locus_tag="ACHY5W_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS4449553.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGGNLPADPKAAPVTENDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIV"
     gene            complement(780..1526)
                     /locus_tag="ACHY5W_004840"
                     /pseudo
     CDS             complement(780..1526)
                     /locus_tag="ACHY5W_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1523..>1840)
                     /locus_tag="ACHY5W_004841"
     CDS             complement(1523..>1840)
                     /locus_tag="ACHY5W_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4449554.1"
                     /translation="VWREYPLRAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARY
                     LAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG
                     DVS"
BASE COUNT          380 a          573 c          409 g          478 t
ORIGIN      
        1 acaatacgac cggatgaatc gacaatctgc cgaacaatat cccggtgctt gttcagctcc
       61 cgcagcgcgg cgcagacacg ctcccacttc tgaacctgac cttttgccct gcgcagcgcc
      121 aggtttgccc tgcgaaggga cggaaaaatc agctcatctg cttgcgtttc ggtaaacgat
      181 ggcaacggct gcacaatgtc cgccacagtt tctgttttaa tttcttcctg tgttgcggct
      241 tcccggactg gtaacgcagc acctgctggc tgaggaaagg ccttaccatc attttccgtt
      301 accggcgcgg ctttcggatc tgctggtaaa ttaccccccg gcatgcagta acgaaattta
      361 ccgttctgat ttacgcgtgc caggcgcccc gttgctgtta cgaccgccaa cgtggaagca
      421 accttgcgaa tgctaacacc gaacttatcc gccagttcct cacacgtttt agccccatcc
      481 tgaccgataa actcaatcat catgtctgcg gtaacttttt gttcgacctc cccggtcagc
      541 atatcctgtg cttcagattt tactggccgc tcttcggtta cccgggattc accttcgcca
      601 gccagaaacc aggtgtgacc agttttatca acgacgccat ttcttttgag ttcccacagc
      661 tcgttgacag cctcttcacg actgattcca aggcgagctg ccaccacatg tgaagaggct
      721 tttttcagtg ctttcagtgc gtcagatacg gtttccatta aaatttcctc cggacaaaat
      781 tacttcacaa ccctctgatt gctgacattt ggacgccagc tatcccagtt aaacgtcacc
      841 catcgaccac ccttcatggt catgcggtcc ataatcctct caccaagaag tgtactcatt
      901 gcggcatgat tcaggtttgt taacatcccg acactgcaca gtgatgctgt ccggcgatca
      961 attatctggt gtaataccac ctgctcgttt ttcgtctccc gctgaacgcc tatttcatcc
     1021 aggaccagca aatcaacact gcaaagctcc tgtaaaaatt tttccccgga tttgccgttg
     1081 tcgtagctgt catgcaacac gctcatgaca tcagacacgg tgacgataat cacgctgcgc
     1141 cccttcgcca tcagccggtt acccatcgcc gctgcaaggt gatttttccc ggtgccggtt
     1201 ttaccgctga acacaaaatt cgttcacccg gtcatcagtt cgtcagctat ggatttggcc
     1261 tggctcagcg cgtatttttg cccgtcgttc tgcacctgat aattcgcaaa cgagcatttg
     1321 ctgtgcagag gctggatgcc cgaacgattc aggatttttt ccacccgcaa ctggcgattc
     1381 tggcggttaa tctcctcgct gcgttttcgt ccttcagcaa gttgccattc ccgccactcc
     1441 tccaccgtcc ggtacggtgg aaccgccccc tgtggtgcaa gtctgcgaat acgttcaaga
     1501 accccaactg ccgcaatgtt tttcatgaca cgtcacccct gaatcccggc ggtatttcag
     1561 tgtccggttc agaaatgtga ttcacgcaac gctggttgtt cgtgccgctt accgggagca
     1621 accaggggtt ttcaaaattc cggtccggcc caaaaaacgt cgtcgctcgc tgaacaaatt
     1681 ccgttcccgc tttcccggtc gccgccaggt atctcgcgta acgcctcaca ccatccagca
     1741 tggtctctgg tggcaccccc tcgcgcaatc tggccttcca ggcactgaat gcggatttct
     1801 tcgggtttgc tccggcacgc aacgggtact cccgccagac
//
LOCUS       DAZUGK010000388         1831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZUGK010000388 DAZUGK010000000
VERSION     DAZUGK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.391"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..793)
                     /locus_tag="ACHY5W_004842"
     CDS             complement(<1..793)
                     /locus_tag="ACHY5W_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4449555.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVF"
     gene            1062..1178
                     /locus_tag="ACHY5W_004843"
                     /pseudo
     CDS             1062..1178
                     /locus_tag="ACHY5W_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1283..1359)
                     /locus_tag="ACHY5W_004844"
     tRNA            complement(1283..1359)
                     /locus_tag="ACHY5W_004844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1323..1325),aa:Arg,seq:tct)
     gene            complement(1373..1449)
                     /locus_tag="ACHY5W_004845"
     tRNA            complement(1373..1449)
                     /locus_tag="ACHY5W_004845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1413..1415),aa:Arg,seq:tcg)
     gene            complement(1457..1532)
                     /locus_tag="ACHY5W_004846"
     tRNA            complement(1457..1532)
                     /locus_tag="ACHY5W_004846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Ile,seq:cat)
     gene            complement(1561..>1831)
                     /locus_tag="ACHY5W_004847"
     CDS             complement(1561..>1831)
                     /locus_tag="ACHY5W_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449556.1"
                     /translation="WTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNML
                     CSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          450 a          509 c          456 g          416 t
ORIGIN      
        1 taaacacgga gaggtcagca cgaaactgtg tcagcatggc tgtaaacagc gcaggttgct
       61 gtgcgtgggt ggcggcgctc atgtcaaact ctccctgcat ccagcacacc gccagcaaca
      121 catttttcgg gttcttctgt aatgcagctt tagtgcgcgc aatcaggtcc tgatataacg
      181 gtttacccac accccagcgt gccgaatcct ggctggcccc cgtgtccgca ctgaatgtcc
      241 cctcagcgcc ctgggtgaat gcagaaccac cacgacagca tggtaccagc aggatccccg
      301 cgttattcgg gatatacgga agcagttttt tggcaatatg taagccctgg ccgacacagc
      361 cgtactgccc tttgctcagg tctgccttcg gatgattcag cgtactcata tcctgcacat
      421 catgcaggca gtggtcagcc ggaatgatgt cgttatacgt acaactctct ccacccggag
      481 ttaccgtgct gcggcgcgcc agctgtttaa tgcgcggatc cggagcatcg taagaatccg
      541 gtaacggaag cccttcaccg taggccatgc cgttggactg tccggcaagc acaaccacgt
      601 agaaccagtc cggctcagat gaagggccga cctgtggatc tccttcaata gccaccgcct
      661 gcatcagtgt gtacggcgta atggcaaccg gtccgccgta tggctgccag ccctctttca
      721 gtttgtgtgt cagcttttcc gcaaggtctg acggcgacgc cgccctgaca acatcatagt
      781 gtttaaatgc catgaatcct cccggccggg ataatattgt gagtaaaatg aggagcgggc
      841 tgaagtccgg aagttacagg acaatggcag aagagagaca acagcccgca atacgaaaaa
      901 ggccgcgcta ttgcgcagag tgattactgt cggatattat tcgccagctg aaatattact
      961 tcacgttttg ttgtttattc cttgccgccc gcgtctccct gcgcgggctt tttttgtcca
     1021 taagaaagcc cctccggaga ggggctggag agtggcgcta tgtgccattg catggtgccg
     1081 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
     1141 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
     1201 gcaaaccaga caacagtcaa ccgcctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa
     1261 agcgagccag ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc
     1321 ttagaaggca attgctctgt ccggctgagc taacaacgca ggatacagat aatggaccgc
     1381 cttcggggac ccgaactccg cgcaaccagc ttcgaaagct ggcgctcttt cctgatgagc
     1441 taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
     1501 gtcgctcgct ctcaccactg agctaaaggg ccgggtcaaa aaataataat cagatgaaaa
     1561 tcaataatca agcccttgcc tggatacata tctgtctggc gggaagccat aatagcggtg
     1621 aaatacagaa ataaagtagg accagcttga ataaccgcat ttttctgcta cagcctgtcc
     1681 atatccatgc cgggaacata acatattcac agcaacacgc atccgctctt ccagcaacaa
     1741 gcgactgaac atgtgccctt catttttcag ttttatcttt aacaaactct cactcatatg
     1801 caggcgtaac gcaatcgcac caagcgtcca g
//
LOCUS       DAZUGK010000389         1827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZUGK010000389 DAZUGK010000000
VERSION     DAZUGK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.392"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHY5W_004848"
     CDS             complement(<1..270)
                     /locus_tag="ACHY5W_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449557.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEE"
     gene            complement(242..793)
                     /locus_tag="ACHY5W_004849"
     CDS             complement(242..793)
                     /locus_tag="ACHY5W_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449558.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWVLKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPSVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQISARGSYEYRCTTMG"
     gene            complement(777..1004)
                     /locus_tag="ACHY5W_004850"
     CDS             complement(777..1004)
                     /locus_tag="ACHY5W_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4449559.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHS"
     gene            1082..1489
                     /locus_tag="ACHY5W_004851"
     CDS             1082..1489
                     /locus_tag="ACHY5W_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449560.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSTLDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            1682..>1827
                     /locus_tag="ACHY5W_004852"
     CDS             1682..>1827
                     /locus_tag="ACHY5W_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS4449561.1"
                     /translation="MDTLNLGNNESLVCGVFSNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNS"
BASE COUNT          493 a          485 c          364 g          485 t
ORIGIN      
        1 ttcttctaca ccgataagtc ggtacaccac aatttgcttt gttctgcctt ttctctcacc
       61 ggtatcaaca attaacccaa tctccatcag gtgtcgtaag ctgtcctgca cagtcttttt
      121 gttcagttcc gttacttctg ccagtgcaga tacagacggg tatgcacaca aatcggcacc
      181 gcacatatca gcaagccagg tcaatacaga cttactggat gaactgccgg ttttcacctt
      241 tttagcccat cgtagtgcat cgatactcat acgaacccct ggcagatatt tgtttatctg
      301 caaagtaata ttgatactgc tgacgatacg catgcttgaa agcaatagct ttttctataa
      361 gctcgtcagt ctcacgttcc acaacagatg gatccgcaaa aagcagtccg gactccacca
      421 catcgccata ttctttgttt aacccggcga tcatgtacgt gatgcttttt ccgtcactaa
      481 tttcacgata caacctgaaa tcattaatcc ggatagcctc cataattgca ggcactagcg
      541 ccgtgaactt ttcacgctta tccctggtgt cgatagcctt ccagcgttcg aatatcttca
      601 ctcgattaac gccaagcgct cgctgatcaa ccgcgccacc ttcatctgtg acacgctgaa
      661 catcgatgtt cgggcgctct ttcaaaaccc agaatgcttc agtgattaat atcgtcgcct
      721 gctcctgtgt cattcctggt cgacatatcc aggcatccag agcctcacga gcctgttcag
      781 gagtgatttt cattgttcaa ccgccccgcc cgctttgcct tacgatattc gtcataaact
      841 ttggggtcgt actgaagttc cccgccggat gcctcttgca ggcgcatcgc gcgaccttca
      901 ggaactagct cttgccattg agaaacagca gatgggtcaa cgccagcagc tttcgctact
      961 ttggctttcg taccgtaaaa attaattacg tctgatttaa acatcgcacc tccaatattg
     1021 agttttctca atgctaatca ctcaaggaat ctcaagtcaa gggttattaa gatatctaaa
     1081 tatgaacgag aaaactttag gtcaacgcat tagagaaaga cgtaagcagg taggcttaag
     1141 tcaaaacggt ttaagcaaag ctgctggcgt atctggctca tcaatttcat tatgggaaag
     1201 tgaccataca gccccgcgcg ggcaaaattt gcatcgcctc gccgaggtat tgcaatgttc
     1261 accaacctgg atactgtttg gcgacgagga caaaacacca gctccccctg tttcactcga
     1321 tagtacccta gacttatcgg aagatgagtt agagatgctg cgtttgtatc gcgcacttcc
     1381 taaatcagag caacaagcac aactcagcga actccgcgcc cgcgttgaga attttaatcg
     1441 cctgttcacc gagctattag aggctcgcaa acgcaacaag catcaataat catcccttca
     1501 caaaatttta aagccttaca tttcaatgta ttggctttat cttgcgccaa tacttgagtt
     1561 ttctcatcaa aaaaccttga cgaaaaataa tgagaaaact aaattatctc catcaacaca
     1621 ccgcacggtg ttctccgcaa acagttccgc taccccggcg ttaaggggaa atgaggtcaa
     1681 catggatacg ctcaatcttg gcaacaacga atctctggta tgcggagtat tctccaacct
     1741 ggacggcacg tttaccgcga tgacgtatac cagaagcaaa acgtttaaaa cggaagctgg
     1801 cgcgcgtcgc tggttagcaa gaaactc
//
LOCUS       DAZUGK010000390         1824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZUGK010000390 DAZUGK010000000
VERSION     DAZUGK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.393"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..351
                     /gene="bssS"
                     /locus_tag="ACHY5W_004853"
     CDS             97..351
                     /gene="bssS"
                     /locus_tag="ACHY5W_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFS4449562.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            466..1584
                     /gene="solA"
                     /locus_tag="ACHY5W_004854"
     CDS             466..1584
                     /gene="solA"
                     /locus_tag="ACHY5W_004854"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFS4449563.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQNKKSDFDLTSFRLSRFQ"
     gene            1632..1745
                     /locus_tag="ACHY5W_004855"
     CDS             1632..1745
                     /locus_tag="ACHY5W_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFS4449564.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          474 a          439 c          436 g          475 t
ORIGIN      
        1 gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
       61 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
      121 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
      181 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcag
      241 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
      301 gccaaaattg aatccggtga tttccaggta aacgagtatc ggcgtcatta acatgacatg
      361 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
      421 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
      481 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgcgccggt
      541 ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc
      601 gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc actggtcctc
      661 cgcgcgcaaa cactgtggga tgagctctcc cgccacaacg aagatgatcc catttttgta
      721 cgctctggtg tcattaacct cggcccggct gactccgcat ttctcgccaa cgtcgcccac
      781 agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
      841 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggtttt
      901 ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
      961 caactgttca actgcccggt caccgcaatt cgtcatgacg atgatggtgt aactattgaa
     1021 acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
     1081 gacctgctcc cggagctgcc tgtccagccc gtacgtaaag tatttgcctg gtatcaggcc
     1141 gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact gcccaatggc
     1201 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
     1261 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggc cagtgatggg
     1321 tcagaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
     1381 gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccgac
     1441 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
     1501 ttaggggaaa tagctgccga ttttgcgcaa aacaaaaaaa gcgattttga tttgacctca
     1561 ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
     1621 aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga
     1681 tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat gtgttttatt
     1741 tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga
     1801 aaaattgaat gagattattc ctgc
//
LOCUS       DAZUGK010000391         1823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZUGK010000391 DAZUGK010000000
VERSION     DAZUGK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.394"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..638)
                     /locus_tag="ACHY5W_004856"
     CDS             complement(<1..638)
                     /locus_tag="ACHY5W_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4449565.1"
                     /translation="MTYFTNTAVNIEGNRKLRTPQIEAYIKIREFFSDPEHREALVIL
                     PTGTGKSGLISIAPYGVSQKRVLIITPGLVTKDSIRKTQEVLEDNFWINYDIVFSSKD
                     IPVVSEYTPDISSEHLEQSHIIFSNIQRISGNRKSALINRVPEDFFDFIIVDEAHHAP
                     AQSWRDALKYFSKAKVLHVTGTPYRGDAQEIPGEIIHETPLSEVMRAKYVKW"
     gene            complement(648..1811)
                     /locus_tag="ACHY5W_004857"
     CDS             complement(648..1811)
                     /locus_tag="ACHY5W_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449566.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDTARKQVANGIDPVEERKAQKLAQKLSTENSFEA
                     VSREWYAAKADRWTVAYREEIIRTFEHDVFPFIGKRPISEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAAPKQKHHPFLTAEELPYFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWEEVDLEKGIWEIPAERMKMRRPHLVP
                     LSTQVIDLFNQLKPITGHYPYIFIGRNNRRKPISKESVNQVIELLGYKGRVTGHGFRH
                     SLSTILHEKGYNSNWIELQLAHVDKNSIRGTYNHAQYLLQRKEMLQWYADFLM"
BASE COUNT          467 a          372 c          350 g          634 t
ORIGIN      
        1 aaccatttta catattttgc tctcatcact tctgacaatg gtgtttcatg aattatttct
       61 cccgggattt cttgagcatc accacgataa ggggttcctg tgacatgtaa aacttttgct
      121 ttagagaaat atttaagagc atcacgccag ctttgggctg gtgcatgatg tgcttcatca
      181 acgataataa aatcaaagaa atcttctgga actctattaa ttagtgcgct ttttctattt
      241 ccagatatac gttgtatatt agaaaatata atgtggcttt gttctaagtg ttcagaagat
      301 atatctggtg tatattcact aactacagga atatctttac tgctgaagac aatgtcataa
      361 tttatccaaa agttatcttc taatacttct tgtgtctttc taatactatc tttggtaaca
      421 agacctggtg taataatcaa aactcttttt tgagatacac cgtatggtgc aatagaaatt
      481 aacccacttt tacctgtgcc tgtaggtaga atcacaagag cttcacggtg ctcagggtcg
      541 ctgaaaaact cccttatttt tatataggcc tcgatttgag gagttcttaa ctttctgttt
      601 ccttcgatat ttactgctgt gttagtaaaa taagtcatat tttctcctta cattaaaaaa
      661 tcagcatacc actgaagcat ttctttccgt tgtaagaggt actgtgcatg gttgtaagtg
      721 cctcttatgc tgtttttatc aacatgtgct agttgtagct ctatccaatt gctgttatag
      781 cctttctcat gcaagattgt cgataaacta tgtctaaaac cgtgtcctgt aactcttcct
      841 ttatacccga ggagttcaat tacttgattt acactttcct tactgatagg ttttctgcgg
      901 ttgttccttc ctatgaagat gtatgggtag tgtcctgtaa ttggttttag ttggttgaaa
      961 agatcgatta cctgcgttga caaagggaca aggtgaggac gacgcatttt catacgttct
     1021 gcaggtattt cccatatccc tttttctaaa tcaacttctt cccaagtggc aaagcgcatt
     1081 tcctgtgttc ttacacctgt aagcatcaca atcttggtcg catttttagt gatgatactg
     1141 cccgtgtaag cttcaagatc tctgacgaag taaggtaatt cttctgcagt gagaaaagga
     1201 tgatgttttt gctttggagc ggctaacgct atagctaaat caggtgccgg gttgtattca
     1261 gcgcggccag tgatgatcgc gtaacgaaac acttctccgc atctttgtcg tacttttctt
     1321 gttttctcca atgccccacg tttttctata cgcttaagta cttcaagcaa ctctagcggt
     1381 ttaatttcag atattggtcg tttaccaata aaaggaaaaa catcatgttc aaacgtccta
     1441 atgatttctt cgcgataggc aactgtccag cggtctgctt ttgctgcata ccattctctg
     1501 ctaacggcct caaaagagtt ttcagttgat aatttctgag caagtttctg agccttacgt
     1561 tcttctactg ggtcaatgcc gtttgcaacc tgtttacgtg ccgtatcgcg tttctcacgt
     1621 gcttccgcga ggcttacaag atcataacta ccaaatgaca ttagccgcgc tttgcctgca
     1681 aaacgaaaac ggaagcgcca tccctttgag ccgtctgggt tgatgagtaa tgacaagcct
     1741 tgcccatcat tcaatgtata tggtttgtct tgtggttttg cgcgtttgat ttgtatatcg
     1801 gttagagcca tatgtataac taa
//
LOCUS       DAZUGK010000392         1822 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZUGK010000392 DAZUGK010000000
VERSION     DAZUGK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.395"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..270
                     /locus_tag="ACHY5W_004858"
     CDS             <1..270
                     /locus_tag="ACHY5W_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449567.1"
                     /translation="YEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKR
                     ETRLADDVRILRQEERERRRNIEKLIQIERNVKARIRRVTKSRTE"
     gene            319..531
                     /locus_tag="ACHY5W_004859"
     CDS             319..531
                     /locus_tag="ACHY5W_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449568.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
     gene            616..939
                     /locus_tag="ACHY5W_004860"
     CDS             616..939
                     /locus_tag="ACHY5W_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449569.1"
                     /translation="MAVANCYPNSEMARVEGELFKIALASLEAEPIAWECGENIILFN
                     PDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYAEGWNDCREAML
                     QGDKS"
     gene            936..1289
                     /locus_tag="ACHY5W_004861"
     CDS             936..1289
                     /locus_tag="ACHY5W_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS4449570.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQYFGAPIVCEA
                     THWMPLPEPPQEVRQ"
     gene            1405..1509
                     /locus_tag="ACHY5W_004862"
     CDS             1405..1509
                     /locus_tag="ACHY5W_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449571.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            1755..>1822
                     /gene="hokD"
                     /locus_tag="ACHY5W_004863"
     CDS             1755..>1822
                     /gene="hokD"
                     /locus_tag="ACHY5W_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS4449572.1"
                     /translation="MKQQKAMLIALIVICLTVIVTAL"
BASE COUNT          531 a          362 c          495 g          434 t
ORIGIN      
        1 tatgagccat cggaaagacc atattgccat cacgcctctg tccgggttga cgaaaaagag
       61 cgtcaggtcc gctgtaaaat ctgcggtgca gttgtggagc catttgactg gatgctctct
      121 gtggcgaaaa gagaaaccag actggcagat gatgtaagga tcttgcgcca ggaggaacgg
      181 gaaaggcgga gaaatataga aaagctaatt cagattgagc gtaacgtgaa agcgcggata
      241 cgcagggtga caaaatccag aactgaataa ttaaatttag ctctgttaaa aatttaatcc
      301 ttaaccgggg gtatatcaat gtcaaacgca cagaaagtta ttaacgctga aaaatataac
      361 gagtgggtga aaaagttctc agagcagatt tttaaaatta ctggcgacga gaatgcggca
      421 aaaaatgaat tagagccgtg gacacctgaa ggtgtcgacc caaattattg ctggtgggat
      481 gttgatccag ttgatgctgc aaatgaagct atgagttatc acaacgatta atgtcaggag
      541 gccgcccgaa agggcggtaa tgaaaagtga ctgaattaac caaaagagaa attaatcgaa
      601 gaagccaaat taaaaatagc ggttgcgaac tgttacccta attcagagat ggccagggta
      661 gagggtgagc tattcaaaat tgcactggca tcgctggaag cagaaccgat agcgtgggaa
      721 tgcggtgaaa acataatcct gtttaaccct gacacagttg aagcatacgc aaaacgtgcg
      781 gaaatatcac ctaaaccact attctccgcg ccgccagcgc tggtagtgcc tgataagttg
      841 ccgcgtgaat acagaaacgg ttggcctctt gcgtatagtg attatgctga aggctggaac
      901 gactgccgcg aagccatgct tcagggagat aaatcatgat taatcgtatc aagctggagc
      961 acatcctcga atatgccagg cagcagaggc atattggtca gcattgtaaa attccaccag
     1021 gagatatggt tgaaatcatg gagattgcca tgcgcaaggc tggcaattct ccggtaactc
     1081 cggatggttg gataagctgt agtgagcgaa tgccgccagt tggtgttgat gtacaactgt
     1141 ttgtgtccag cacgggtgaa cagtttacag gttttaacct ggataatacg ggtgacttcc
     1201 agtatgcgca gtatttcggt gcgcccatcg tgtgtgaagc cacacactgg atgccgctac
     1261 cagaaccgcc gcaggaggtg cgccaatgaa ctggcctgaa gcatttgcaa ttacaggcgt
     1321 tgctatggct atcgcttttt tagtatatgt tatttgtcgg tgggggtaaa aacgttcgcc
     1381 gggattaaca ccaaaggagg gaatatgtcg gatgatattt cactggcaat ggaaggtgcg
     1441 ctggctgtta ttgctgttgt gggcgtttac tgcctggttg tgtttttgat ggatcgacta
     1501 gggaactgaa ttcattacga tatgggaatt cccatatcgg gtaaaaacgg tttgcggtaa
     1561 agcgagagtt aagtagaatt gctgcgggtg cttgaggcta tctgcctcgg gcatgccacc
     1621 gtaaggcaga cagagaaaag ccccagttaa cattacgcgc ccggcaagac gcttaacatt
     1681 aatctgaggc catatctatg ctctacacac gtaggttagc ctcttacgtg ccgaaaggca
     1741 aggagaagca ggctatgaag cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt
     1801 taaccgtcat agtgacggca ct
//
LOCUS       DAZUGK010000393         1821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZUGK010000393 DAZUGK010000000
VERSION     DAZUGK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.396"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..357
                     /gene="espZ"
                     /locus_tag="ACHY5W_004864"
                     /gene_synonym="sepZ"
     CDS             58..357
                     /gene="espZ"
                     /locus_tag="ACHY5W_004864"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFS4449573.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
     gene            441..818
                     /gene="sctI"
                     /locus_tag="ACHY5W_004865"
     CDS             441..818
                     /gene="sctI"
                     /locus_tag="ACHY5W_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFS4449574.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            821..1393
                     /gene="escJ"
                     /locus_tag="ACHY5W_004866"
     CDS             821..1393
                     /gene="escJ"
                     /locus_tag="ACHY5W_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFS4449575.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1399..>1821
                     /gene="sepD"
                     /locus_tag="ACHY5W_004867"
     CDS             1399..>1821
                     /gene="sepD"
                     /locus_tag="ACHY5W_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFS4449576.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPT"
BASE COUNT          614 a          356 c          358 g          493 t
ORIGIN      
        1 taatttggtt atttataacc aagtaaatgt ctaaattaga caaaaggaga aagatcaatg
       61 gaagcagcaa atttaagtcc ttctggtgca gtaatgccgc tggcgacctc actcagtgga
      121 aataactcag tggatgagaa gacaggagtg attaaaccag aaaatggaac aaatcgcacc
      181 gttagagtta tagccggatt agcacttacc actacggctc tggcagctct aggtacaggt
      241 attgcagcgg catgctcgga gacgagcagc acagaatact tagccctggg tattacttct
      301 ggtgtactag gtactcttac tgcggttggc ggtgcattag cgatgaaata tgcctaaccg
      361 gcccatcgca taaataaaag atagaggtaa tggatgtatt atgcccttgc ctctttcata
      421 aaaagaggct aacagtaaat atgaacaaca ttaatcaatc ggaaaacatt aatattcaat
      481 taaataaagc gccccaaaca aatttcgttg atgagcatac ctcgttagcc tcagccccct
      541 ctgcggcagg tgcggctcag tttcttgatc aattactccc taaaacagcg ggagtgtctt
      601 ctccagaaca agtgttgatt gaagaaatta aaaagagaca tcttgcaaca atgaacagcg
      661 atctcagttt cgatgctcta tctgcaggtg ggctctcgcc agaagacgtg ctcaccttac
      721 aaaagaatgt gctcaacgca aacgtcaacg ttgatgtcgt atctaagtta gcaagcctcc
      781 tttcaacatc ggttacaaaa ttagtttcga tgcaataaaa atgaaaaaac acattaaaaa
      841 ccttttttta ttggctgcta tttgtttgac agtggcatgt aaagagcaat tgtatacagg
      901 attaacagaa aaagaagcta atcagatgca agcactgttg ctatccaatg atgtcaatgt
      961 ttccaaagaa atggacaaaa gtggaaatat gacactcagt gtggaaaaag aggattttgt
     1021 tcgcgcaata acgattttaa ataataacgg cttcccgaaa aagaaatttg cagatatcga
     1081 ggttattttc ccacctagcc agttagtggc atcgccatca caagaaaatg caaaaatcaa
     1141 ctatctcaaa gaacaagaca tcgaaaggtt gctaagtaaa atacctggtg ttatcgattg
     1201 cagcgtttca ttaaatgtta acaataacga aagtcaacca tcctcagcag ccgttttagt
     1261 catcagctcg ccagaggtta acttagcacc atcggtcatt cagataaaaa acctagttaa
     1321 gaatagtgtt gacgatctca aattagaaaa tattagtgtt gttatcaagt catcctcagg
     1381 acaggacggg taatatgtat gaacaataat aatggcatag caaagaatga ttgtgactgg
     1441 ctaacggccc ttgattttgt aaaggatgtc aacggttcac caacgcatct cactttttat
     1501 atctaccaaa aaaatgcttt cttgcacgat tttggaaact actgggttct atatattgag
     1561 cttagtggtg attttcgtca ggttccaaca gatacattta tcagactctg caatatcctg
     1621 gccgttagta acgaatataa acaaatgggt attttcctgt ctaataagaa gtggtatctt
     1681 tgccaaattt ttcataaaga taataatcat cgcgcaaaca tgtcaaaagc aataatgcaa
     1741 catactctcg cctctttgct cgataagcaa tttgataaac tagagcaatt atcttcatcg
     1801 gatacaatga tgccgccaac a
//
LOCUS       DAZUGK010000394         1784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZUGK010000394 DAZUGK010000000
VERSION     DAZUGK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.397"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..401)
                     /locus_tag="ACHY5W_004868"
     CDS             complement(<1..401)
                     /locus_tag="ACHY5W_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449577.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTL"
     gene            complement(373..924)
                     /locus_tag="ACHY5W_004869"
     CDS             complement(373..924)
                     /locus_tag="ACHY5W_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449578.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(908..1135)
                     /locus_tag="ACHY5W_004870"
     CDS             complement(908..1135)
                     /locus_tag="ACHY5W_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4449579.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            1212..1619
                     /locus_tag="ACHY5W_004871"
     CDS             1212..1619
                     /locus_tag="ACHY5W_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449580.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          481 a          463 c          344 g          496 t
ORIGIN      
        1 ctaagagttc tgtttttggg tctgttttgt gctgaaacat aaccattttc ggtactgttt
       61 ttattaacag caccaatttt acccaccttt aaagactccc gtttttgggt gtattcaggc
      121 tcggcaacac tttcttctac accgataagt cggtacacca caatttgctt tgttctgcct
      181 tttctctcac cggtatcaac aattaaccca atctccatca ggtgtcgtaa gctgtcctgc
      241 acagtctttt tgttcagttc cgttacttct gccagtgcag atacagacgg gtatgcacac
      301 aaatcggcac cgcacatatc agcaagccag gtcaatactg acttactgga tgaactgccg
      361 gttttcacct ttttagccca tcgtagtgca tcgatactca tacgaacccc tggcagacat
      421 ttgtttatct gcaaagtaat attggtattg ctgacgatac gcgtgcttga aagcaatagc
      481 tttttctata agctcgtcag tctcacgttc cacaacaact ggatccgcaa aaagcagccc
      541 ggactccacc acatcgccat attctttgtt taacccggcg atcatgtacg taatgctttt
      601 tccgtcagta atttcacaat acaatctgaa atcgctgatc cggatagcct ccataattgc
      661 cggaatcagc gccgtgaatt tgtcacgctt atctctggtg tcgatagctt tccagcgttc
      721 gaatatcttc acccggttaa cgcccagcgc ccgttgatca acctcgccat cattaaacgt
      781 gacgcgttga acatcgatgt tcgggcgttc tttcagagcc cagaatgctt ccgtgattaa
      841 tatcgtcgcc tgctcctgtg tcattcctgg tcggcatacc caggtatcca gagcctcaca
      901 aacctgttca gtggtgattt tcattgttca accgccccgc ccgctttgcc ttacgatatt
      961 cgtcataaac tttggggtcg tactgaagtt ccccgccgga tgcctcttgc aggcgcatcg
     1021 cgcgaccttc aggaaccagt tccccccatg cagcaacgct tgccagccta actcctgcga
     1081 cattggcaag ctttgttttg ctgccaaaaa acgctatagc atcaattttc aacatatcga
     1141 cccccttaga ttttcctaag gaaactagat cgtagagaaa cctaagtcaa gaaaaattag
     1201 aattacctaa tatgaaaaac gaaaccttcg gtgctcgcct cttatacagg cgaaaaaaat
     1261 taaaactgtc tcaggccgca ttaggtaagc tggtcaaagt ggctcacgta acaatttctc
     1321 aatgggaaag agatgaaaca cagccagcgg ggaagagatt attcgcactg agccaggcgc
     1381 ttcagtgctc gccgacttgg cttctttttg gagatgaaga taagcaacca ggcgaaccga
     1441 tcccagataa tcagccagtc aatctgacag aagatcaaaa agagttgctt caactgttcg
     1501 acgcactgcc tgagtcagag caaaaggctc tgttgtcaga gatgcgtgct cgagttgaga
     1561 atttcaacaa actttttgaa gaactactca aagctcgcaa aagaagcgca aataaataac
     1621 cccccttttt ttcgcaactc tctgtaataa aaagcacaaa ctttcaaata cttgtgtttt
     1681 ttacatcaag aagcttaggt ttttctacac aaaaagctta accataactc ttaggctatt
     1741 ctaaattcta ctcatcaaga caccgcacgg tgttctcagc aaac
//
LOCUS       DAZUGK010000395         1755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZUGK010000395 DAZUGK010000000
VERSION     DAZUGK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.398"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(479..1456)
                     /locus_tag="ACHY5W_004872"
                     /pseudo
     CDS             complement(479..1456)
                     /locus_tag="ACHY5W_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          439 a          408 c          393 g          515 t
ORIGIN      
        1 gaaaatctta ttattaacac ccttaaccaa ggtgctaatg tgataaaaaa atatttatct
       61 cgatgctacc caacatacga ctcatttatt tttctcggtt tctccttgta tatatatgtt
      121 gctttataat tttactacgc acgtcaggcg tatctgtaac atagctccgc attatgcaat
      181 gcgactttgc atacaaatag gaataaatct aatagctgta gatttgctcc tgtatttcct
      241 gaaccaagcg agccagtaca tgatgcatat gtctggaaaa taattcagag taaggataag
      301 tccagtccag ctttgttgaa taaatcaggt ttgggataag ccccctcagt tggtagctac
      361 tgcaggtaat acgcacgtat tatgacattc gtgccctcgt cattttgttt agtacacgca
      421 ctgtagtggc acagaacttc tgcgacctga ccatcctaaa tagctgcgcc gaataccgct
      481 acacttttaa cagcccatat ttaccaccgg ggaatgggct cacggtttcc ataaaataat
      541 gaaccttttt aagcagttac cacattgaac ggcactgatg attgcgcgtt atcgtctcgt
      601 gaagcgcctg ctataaccgc tcaacatgat tcacccacgg cgagtaaact ggctgataaa
      661 tcactctgaa cttcggattt tctttcagcc agcgctgtgt ttcccgactt ttatggatga
      721 tgtagttgtc cactatgagc gtgataggtt tatcccgtcg gtatgtcaat ttcagatgtt
      781 tcaacagact gataaataag gctgagcttt tgctgttgcc acccacatag ctgactttgc
      841 ccgttccact atgtaaagca ccagccagat agtatttttc attctgcccc ggtgttacca
      901 catgtttttg ctgtctgcga agttgccagt ccgcaccggt tttgggatta agatagatat
      961 acacttcatc ttcataaaag accggatgct ccgcactaca ttcgtccagt gctttgtgaa
     1021 ttgctgcttt atgcgggtta cgggtacgca gggttggcgc agccctgcgc cacacaagcc
     1081 cgactaatgg caaccagcgg cgaacggttc cggcatgtaa ctggcactcg gttatctcgt
     1141 tgatttttat tgccagcagt tctgtactcc agcgtgaacg ctgatagcca aaatatccgg
     1201 gagagtgttt tatcagttca cgaaacaatg tgcagatatg ctcaaacggc cagcggcggg
     1261 aacgtcctgc gggtaatgat ttcagacctt caacactcga cagcgtgaac cagttaatcc
     1321 agcgcctgac ggaggaacgg gcgcagcaga gcgttctggc aacgtcactg acgcgagcgc
     1381 tccggtgcag catcagcatg gcggtcagcc tgcgggcata atttttatcg tgagttttat
     1441 agattgcttt ttgcatcagg cgtcattcgt cgcggggaat tggtgctatg atcggcatcg
     1501 ctcagtccgg ttaggggggg atttttggcg attgatcaga tcgcacaatc cgggctgagt
     1561 tccctttcat tgatctacta ttcggcgcag ctatttagtt ggtgattttg aacttctgct
     1621 ttgctactaa gcagcctgcg ttttcaggaa aatacgtgat gtgatcctgc aactcagcaa
     1681 cagttcggtt gattcaacaa agcgtttttt aacaataatt cttcattgta aaaagcatct
     1741 tcagttgtga tcaaa
//
LOCUS       DAZUGK010000396         1755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZUGK010000396 DAZUGK010000000
VERSION     DAZUGK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.399"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..552)
                     /locus_tag="ACHY5W_004873"
     CDS             complement(<1..552)
                     /locus_tag="ACHY5W_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS4449581.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALA"
     gene            complement(773..1183)
                     /locus_tag="ACHY5W_004874"
     CDS             complement(773..1183)
                     /locus_tag="ACHY5W_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449582.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(1199..>1755)
                     /locus_tag="ACHY5W_004875"
     CDS             complement(1199..>1755)
                     /locus_tag="ACHY5W_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449583.1"
                     /translation="ELPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNA
                     EAATVKQALSCNPEAWAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGY
                     MDGPGGGITPVNINGTVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAY
                     LVWFGEIGVHEFAYLVKNYVAAVN"
BASE COUNT          426 a          435 c          423 g          471 t
ORIGIN      
        1 tgccagcgcc tggcgtgccg gtggcggcag gaccagcgac tgcatggcat cacgcttctc
       61 ctctatccgg tcaaacacgc ccaccacctc gtaagcccct tcgataactt tctccactac
      121 atttccccgg tgtggaacac gcacttcccc cagagactgg ccacagacgc acccgttctg
      181 gcagacgaac ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt catgagagtt
      241 gagcagaata atttcaggga catgttctcc gtttatctct ccggcccgcc gcagacgcag
      301 catgtgtttt gtgtatcccc ggcggcccgg gtcgcgcaca cgggtctggc aggcgaagaa
      361 cggctgaaag ccttcccgct gcagattttc caggacggta atggtgggaa tgtacgcata
      421 ccgttcactg cgggaggtgt gccggtcttc cccaaaaata ctgggtacat ggcgtatcag
      481 ttcttcatgt gtcagcggac ggtcacggcg tatctggttt gcagcataac caaaacgact
      541 tgctaatcgc atactttgct ccttatcggt ggttaagatt tactggtgca ataaatgaaa
      601 aaagccgcgt ctcccggaga agacgcggcc tgacgggtga aatgaataag gtttattgtc
      661 tgagaggcct ttcactggcg agccacgcat taagcggctt tccggtaaca atggctgggg
      721 ctgtatttac ctttgatatt aataatgtgc agagaaacgc atggtttact ccttagattt
      781 taagtttgtc ctgggctaca acgacaggtt ttccggatgt gtcgatgact cctaccgtcg
      841 gtacgccatt tttgatttcg aagaacggtt tgttgctacc tgagcacagt tgattgtccc
      901 cgtattcaat catttgtgct ttcccggttt tcaggttaac taccgagaca gtgtagtcgt
      961 agcaccaggc accaccacgt tcaagcacga aaacatagct gttatcttta tagttgaact
     1021 gattctttgt ctggcaattc ataccgtcaa tttcggtaca ggaaaacgct tctgtagtat
     1081 taacgtattt gatgttattt ggcagtttta cttgtttcgc agtagcgaag acagagaaca
     1141 gaatagttac agcaacgagt aaagttttga taaaagtttt catgatagac tccttaaatt
     1201 agttaaccgc agctacgtag tttttcacca gatacgcgaa ttcatgcacg ccaatttcac
     1261 cgaaccaaac caggtaggcc gggtcatcta aaccaaagta acggtagcca acaacttcat
     1321 gggtttttgg gttatagagt gttacgataa agtcccagcc gcaggtattg gctttgcaac
     1381 cgtcgccctg gaaccagact gtaccgttaa tgtttactgg cgtgatacct ccaccagggc
     1441 catccatata gccgccttta ccgaacatgc cgctcagtcc gaccggtttt aatgctttgt
     1501 cgatagcggc tttgtaggta gcgtcacctt cgtacaactc gtacgttgat ggacacgctc
     1561 caggttgttc agcccaggct tctgggttac aactaagagc ttgttttacg gtagccgctt
     1621 ctgcgttaaa gttaaagccc gtgaacagaa ccgccagaaa taacataagg gtatgtaaac
     1681 gtttcattgt aaatattctt ccgttgtaaa tggcgctgga aaatttctca tcagtgaaac
     1741 cttccggaag ttccc
//
LOCUS       DAZUGK010000397         1709 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZUGK010000397 DAZUGK010000000
VERSION     DAZUGK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.400"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..>1709
                     /gene="tir"
                     /locus_tag="ACHY5W_004876"
     CDS             45..>1709
                     /gene="tir"
                     /locus_tag="ACHY5W_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFS4449584.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAA
                     SATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAK
                     QQAIENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQ
                     PVEQTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDT
                     SSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGAR
                     LLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPG"
BASE COUNT          478 a          372 c          453 g          406 t
ORIGIN      
        1 taaaggattg ttatctattt acataaaata aaaaggagat atttatgcct attggtaatc
       61 ttggtcataa tcccaatgtg aataattcaa ttcctcctgc acctccatta ccttcacaaa
      121 ccgacggtgc aggggggcgt ggtcagctca ttaactctac ggggccgttg ggatctcgtg
      181 cgctatttac gcctgtaagg aattctatgg ctgattctgg cgacaatcgt gccagtgatg
      241 ttcctggact tcctgtaaat ccgatgcgcc tggcggcgtc tgagataaca ctgaatgatg
      301 gatttgaagt tcttcatgat catggtccgc tcgatactct taacaggcag attggctctt
      361 cggtatttcg agttgaaact caggaagatg gtaaacatat tgctgtcggt cagaggaatg
      421 gtgttgagac ctctgttgtt ttaagtgatc aagagtacgc tcgcttgcag tccattgatc
      481 ctgaaggtaa agacaaattt gtatttactg gaggccgtgg tggtgctggg catgctatgg
      541 tcaccgttgc ttcagatatc acggaagccc gccaaaggat actggagctg ttagagccca
      601 aagggaccgg ggagtccaaa ggtgctgggg agtcaaaagg cgttggggag ttgagggagt
      661 caaatagcgg tgcggaaaac accacagaaa ctcagacctc aacctcaact tcaacctcaa
      721 cttccagcct tcgttcagat cctaaacttt ggttggcgtt ggggactgtt gctacaggtc
      781 tgatagggtt ggcggcgacg ggtattgtac aggcgcttgc attgacgccg gagccggata
      841 gcccaaccac gaccgaccct gatgcagctg caagtgcaac tgaaactgcg acaagagatc
      901 agttaacgaa agaagcgttc cagaacccag ataatcaaaa agttaatatc gatgagctcg
      961 gaaatgcgat tccgtcaggg gtattgaaag atgatgttgt tgcgaatata gaagagcagg
     1021 ctaaagcagc aggcgaagag gccaaacagc aagccattga aaataatgct caggcgcaaa
     1081 aaaaatatga tgaacaacaa gctaaacgcc aggaggagct gaaagtttca tcgggggctg
     1141 gctacggtct tagtggcgca ttgattcttg gtgggggaat tggtgttgcc gtcaccgctg
     1201 cgcttcatcg aaaaaatcag ccggtagaac aaacaacaac aactactact acaactacaa
     1261 gcgcacgtac ggtagagaat aagcctgcaa ataatacacc tgcacagggc aatgtagata
     1321 cccctgggtc agaagatacc atggagagca gacgtagctc gatggctagc acctcgtcga
     1381 ctttctttga cacttccagc atagggaccg tgcagaatcc gtatgctgat gttaaaacat
     1441 cgctgcatga ttcgcaggtg ccgacttcta attctaatac gtctgttcag aatatgggga
     1501 atacagattc tgttgtatat agcaccattc aacatcctcc ccgggatact actgataacg
     1561 gcgcacggtt attaggaaat ccaagtgcgg ggattcaaag cacttatgcg cgtctggcgc
     1621 taagtggtgg attacgccat gacatgggag gattaacggg ggggagtaat agcgctgtga
     1681 atacttcgaa taacccacca gcgccggga
//
LOCUS       DAZUGK010000398         1701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZUGK010000398 DAZUGK010000000
VERSION     DAZUGK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.401"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHY5W_004877"
     CDS             <1..95
                     /locus_tag="ACHY5W_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449585.1"
                     /translation="RRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            92..529
                     /locus_tag="ACHY5W_004878"
     CDS             92..529
                     /locus_tag="ACHY5W_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4449586.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            679..1296
                     /locus_tag="ACHY5W_004879"
     CDS             679..1296
                     /locus_tag="ACHY5W_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4449587.1"
                     /translation="MLASLGGLVSYAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1484..1678)
                     /gene="ylcI"
                     /locus_tag="ACHY5W_004880"
     CDS             complement(1484..1678)
                     /gene="ylcI"
                     /locus_tag="ACHY5W_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4449588.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          445 a          382 c          442 g          432 t
ORIGIN      
        1 tacggcgctg gacatacgct ggcggtaagc aatggaaagg gctgatgact cgccgcgaga
       61 ttgagcgtga agtctgtttg tgggggcaac aatgagcagg gtaaccgtta ttatctccgc
      121 tctggttatc tgcattatcg cctgcctgtc atgggctgtt aatcattacc gtgataacgc
      181 catcgcctac aaagagcagc gcgataaagc cacatccatc atcgctgata tgcagaagcg
      241 gcaacgtgat gtagcagaac ttgacgccag atacacaaag gagcttgctg atgctaatgc
      301 gactatcgaa agtctccgtg ctgatgtttc tgctgggcgt aagcgcctgc aagtctccgc
      361 cacctgtgca aagtcaacga ccggagccag cagcatgggc gatggagaaa gcccaggact
      421 tacagcagat gctgaactca attattaccg tctccgaggt ggaatcgaca agataaccgc
      481 gcaggttaac tacctgcagg aatacatcag gacgcagtgc ttaaaataat tttaatttca
      541 ctgaaattta acaagtgact ttcaggaaaa tgcctcgcag atgcggggca tttttgtacc
      601 ggtatttcac cgcgcaccgc agcgcacaat aaacaccgaa cctgaccctt tggaatgggc
      661 ctttgaggat accagttagt gctggcgagc ctcggtgggc tggtttccta tgcggcaaag
      721 gttcatttca aagaagcagg caacgccatg aatgaattaa ttgcgaatca tgacttcgac
      781 tttcgccagt tagttaccgc agcagaaggt caaccggtaa ctgacacctt ccagattgcc
      841 agggcatttg gtaaacgcca tcagcatgtg attagggcta ttaaatgttt gagatgttct
      901 gaggaattct cgacaaccca tttttgggcc gtcgagaaaa tcaatgactt aggtattttt
      961 gacaagaaac agatttacta ccgcatggac tttagtggct tcgttatgct ggttatggga
     1021 tttaacgggg caaaagccga tgctgttaaa gaagcctata tcaatgcgtt taactggatg
     1081 tcagcagaac tccgtaagta cagcgaaagt tatgaagcag aacgtaacgc cgtaatgctg
     1141 gagtacatga aagagaagga tgtcgccagc atgtcaggcc gtctgctcaa tcgctggggg
     1201 agaacgaaaa aacctcaatt gcttgcaaag ctggaacgtc tggagagaca gggacagttt
     1261 ttattaccgg gattcgataa aggtattcaa gcctgacaca ttatgcgctg tatcgtcgcc
     1321 gtattcccgc attaaccatg accgtagccc gacggggaat tccttctgcg tgagtgtgcg
     1381 ggaataatca aaaacgatgc acaccgggtt ttactgtgct gacagacgca gggttaccct
     1441 catagtcgct tttccggtgc gatggtggaa gaaaccggga tgttcatcca tcatcacttt
     1501 ggattgatgt atatgctctc ttttctgacg ttagtctccg acggcaggct tcaatgaccc
     1561 aggctgagaa attcccagac cctttttgct caagagcgat gttaatttgt tcaatcattt
     1621 ggttaggaaa gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga
     1681 ggttgccccg tattcagtgt c
//
LOCUS       DAZUGK010000399         1699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZUGK010000399 DAZUGK010000000
VERSION     DAZUGK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.402"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..1341
                     /locus_tag="ACHY5W_004881"
     CDS             118..1341
                     /locus_tag="ACHY5W_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449589.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDTATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGAITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIVTRTSSDSN"
     gene            1442..>1699
                     /locus_tag="ACHY5W_004882"
     CDS             1442..>1699
                     /locus_tag="ACHY5W_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449590.1"
                     /translation="MSLSRFLLKIIPSSFLKEDDATQKKSNEFIAEATFGSNLVDGEP
                     TWKWAEEHKDDLEVMKRCCDAELITMKKCGLVPAPYYFERVA"
BASE COUNT          501 a          317 c          414 g          467 t
ORIGIN      
        1 aaaattcttt catgtaactt taaatcgatc aggttgcttt catcaaaaat ctgtacatat
       61 gcttgtacat aatgtacaaa gcggcagagg tgttttgcga tttgtacaag gtgagtaatg
      121 gcgctgtctg atgcgtggtt gcgttcagtc gttggaaagg aacgtgataa ggttttggtt
      181 aaatccgatc gtgatggtct gtctgtcaga gtatcaccga aaggtcgcgt agtgttccaa
      241 tatcgttatc aatgggcagg gaaaggtgag cgtcttgata tcggaactta cccggcaact
      301 ggattaaaag aggccagaga agaagttatc cgtctccgtg gtgaactcga gtcaaaccgt
      361 aatccacgat tggtcaagca ggctgaaaaa cgaaaagcta ctgaagccat gacggtagag
      421 tctgtgatcc gtgcctggta tgaagcatat tgtgtaaaaa ataaaaaggg gtctgaacag
      481 atactccgct cgtttgagct gcacctgttc tctaaaatcg ggaatatccc tcacgataca
      541 gctacattgc atgattggtt agaagtcctg gagcctctta gcactaagac tccagcaata
      601 gcagaccgat tgctaattaa cgcaaagcag gcccatgtct gggcgtataa gagaaagctc
      661 attgaaactc gcccgctgtc ggatatcacg ggtaaagata tggatatccg taaaggtcag
      721 aagaaacggt ttctgacaca tgatgaaatt aaaatccttt atgctgcgat cgatggttct
      781 cgaatggttc ctaaataccg ggccttcatt aaactattgc tgcattttgg ctgccgtagt
      841 tcagagctaa ttaccgccag ggtggacgat tttgatttca ttaataaagt atggactgta
      901 ccaccagaac gacataagac tggggcgata acaggcgaac cgctaaagcg gcccattatt
      961 gaaccggttg aagagcttat aaagtacgct atctctatga acaatggttc cgatatgctt
     1021 tttactaagg aaggaagcag ggaacccgtt ggtcggacat cattgcagtc gctgccttac
     1081 aatttaatgc agtacgcatg gcggcgtttg gggtatcaat tccctcattg gtctcttcat
     1141 gatttgaggc gaacagcacg aacaaacttt tctgatctta ctgcgcctca tattgcagaa
     1201 ataatgctcg gtcataaact gccaggggta tggcaggttt atgacaagag cgattattta
     1261 gaagaacagc gtaaagctta ccaggcatgg tgggagagag ttgaatcgat cgttactcgt
     1321 accagctcag actccaactg acagtttgtg ttgagcgaag agtggaagtt tgtaatgatt
     1381 gatacaactg ataaaggtaa tatttaaatc gaggtatgtt aatcaatgag gagcaggtta
     1441 tatgagtctt tcacgatttt tattgaagat aattccatcc tccttcctga aggaggatga
     1501 tgctactcaa aaaaaaagta atgaatttat tgctgaagcc acattcggtt ctaacttagt
     1561 cgatggagag ccgacttgga aatgggctga agaacataaa gatgaccttg aggtaatgaa
     1621 acgatgctgt gatgctgaac taataacaat gaaaaaatgt ggtttagttc ctgctcctta
     1681 ttattttgag cgtgttgct
//
LOCUS       DAZUGK010000400         1688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZUGK010000400 DAZUGK010000000
VERSION     DAZUGK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.403"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..569
                     /gene="espA"
                     /locus_tag="ACHY5W_004883"
     CDS             <1..569
                     /gene="espA"
                     /locus_tag="ACHY5W_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFS4449591.1"
                     /translation="NATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQAAILM
                     FSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAKLPQD
                     VIDYIKDPRNDISVTGISDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEIQQMS
                     NTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            582..>1688
                     /gene="espD"
                     /locus_tag="ACHY5W_004884"
     CDS             582..>1688
                     /gene="espD"
                     /locus_tag="ACHY5W_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFS4449592.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRI"
BASE COUNT          490 a          336 c          418 g          444 t
ORIGIN      
        1 caaatgcaac atccgttgtt aatgtgagtg cgagttcttc gacatcgacg atctatgact
       61 taggtaatat gtcgaaggat gaggtggtta agctatttga ggaactcggt gtttttcagg
      121 ctgcgattct catgttttct tatatgtatc aggcacaaag taatctgtcg attgcaaagt
      181 ttgctgatat gaatgaggca tctaaagcgt caaccacggc acaaaagatg gctaatcttg
      241 tggatgccaa aattgctgat gttcagagta gcactgataa gaatgcgaaa gccaaacttc
      301 ctcaagacgt gattgactat ataaaagatc cacgtaatga cattagtgta actggtatta
      361 gtgatcttag tggtgattta agcgctggtg atctgcaaac agtgaaagcg gctatttcag
      421 ctaaagcgaa taacctgaca acggtagtga ataatagcca gctcgaaatt cagcaaatgt
      481 cgaatacatt aaatctctta acgagtgcac gttctgatgt gcaatctcta caatatagaa
      541 ctatttcagc aatatccctt ggtaaataac cggagataac tatgcttaac gtaaataacg
      601 ataccctgtc tgtaacgtct ggggttaata ccgcctcggg tacttctggt attactcaat
      661 ctgaaacggg tttatcgctg gatttacaac tggttaaatc catgaactcg tcagcaggct
      721 ggacagaaag tagcccttta ccgacgccgc cggcaggtca ctcattagtg acgccctctg
      781 ctgctgagga tgtccttagt aaattgtttg gtggtattag tggtgaggtt acaagtcgca
      841 ctgaggaggc agagccacag cgcacaagct atccctatct ctctcaggtg aataccgttg
      901 accctcagca aatgatgatg atggtcactc tgttatccct ggatacttcc gcgcagaaag
      961 tctcgagtct gaaaaactct aacgagattt atatggatgg gcaaactaaa gcgctggaga
     1021 ataaaacgca ggagtataaa aaacagctcg aagaacaaca gaaagccgaa gagaaatcac
     1081 aaaaaagtaa aattgttggc caggtctttg gttggttggg cgtcgcatta acagccgttg
     1141 ccgctgtttt taacccagca ctctgggctg ttgttgccat tggtgcaaca gcaatggcac
     1201 tgcaaacggc agtcgatgta atgggggaaa atgcccctca gggattaaag actgcagcac
     1261 aggtctttgg cggaatatct atggccgcaa gcattctgac agccggcgtt ggcggggtgt
     1321 cttcactgtt atctaaattt ggtaatgttg ctaacaaaat tggctcaagc gttgtaaaag
     1381 tcgttgagaa ggcggcagaa gcgctggtta aaaacgtttt tgcaaaaatt tcgacagtgg
     1441 ctgagggcgt tacgaacggt attcgttctg ccgggacaac tgcgttgaat aatgaggctg
     1501 cgcaactcca aatgttgtct cagttagctg ctttcgcggt gcaaaactta actcgacaga
     1561 gtgaaagctt aggtgagagt gcgaagctcg agctggataa agcggcaagc gagttacaaa
     1621 atcaggcgag ctatttacaa agtgtttctc aactgatgtc cgattcagca cgggtaaata
     1681 gtcgtatt
//
LOCUS       DAZUGK010000401         1667 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZUGK010000401 DAZUGK010000000
VERSION     DAZUGK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.404"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..>1667)
                     /locus_tag="ACHY5W_004885"
     CDS             complement(29..>1667)
                     /locus_tag="ACHY5W_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4449593.1"
                     /translation="CPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRIVDAPGSPT
                     FGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNW
                     RQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVV
                     AADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEP
                     PAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYS
                     VDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLD
                     DILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWVHETAVVRRKSEASR
                     FQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGI
                     PDESVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPKGNAILITK
                     QASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          353 a          550 c          412 g          352 t
ORIGIN      
        1 gttcatgatg gttacagggt gagcagggtt atatgaaaaa aacctcatac gctttctttt
       61 tcggttccgg attcagggac atcagggaca ccgcattgaa gagcgccatc agcgggtcaa
      121 tttttccccg tccgctggcc tgtttggtga taagaatggc gttaccttta ggctccaccc
      181 gggcattgcc aacgcaccag gccatcagtg gctgaccacc atgcaccagc actccctcag
      241 ccagtttgcg ctcggtggtt ttgatggccc cgcccagctt ccagccctgg cttatcccca
      301 ccacactctc atcggggatc ccggcttccg ccagtgaatc cagaatctgc cccacacctg
      361 acgggtcaat accgatatga tccagtaact cagcctcatg aatacgacgc acatactccg
      421 ccacttccgc cgtgtcatcc ccgacccgac ggacaatcgt catgtctcca caggccacaa
      481 aatcctgaaa ccgggatgcc tcactcttcc gtctgaccac cgcggtttca tgcacccagg
      541 catggcccca gcccagccat tcgcgggttt ccctgtcacg gccaatcacg tacattccca
      601 gcagatcatc caggcccccg ccgtcaatcc ccaccgtcac cacatcagcg cgctgcagga
      661 tatcgtccag gctgacgcgc ctgccctgct gctcccagaa atccgcccct gcccagcggt
      721 cagaacgcag ggcaagacca atttccacat tggcgtgttt tgacatgaaa ccgcggaaag
      781 tctcttcccc ggcttcccgg gctttacgat actcccggta cagaaaggcc tcatccaccg
      841 aatagccgag attcgggtta accatggcga ggttttccat cagcaggtga gccccgcttt
      901 ccaccatttc aggaggatgc tcaaaaatca ccggcagaaa gtgcggatca tgaattttgc
      961 cgtcacggac atcccgggcg tactgcagtt tctgcctgaa caccccggca ggcggttcat
     1021 tcgactgggt ggttgtgtac accacaaacc cttccggacg tgaagccagc ccgccgatgg
     1081 cttcacgtag catgtcttcc gctttgtact gcttgccaaa cagccacagt tcatcaatca
     1141 gtgtccccac ggacttgata ccggacaccg tattcggatc ggctgccacc accttcaggg
     1201 tggtgtccgt cacccgatgg gtgatggtcc ggatatgtgt ctgcacctga cagaggtcat
     1261 ccagatcatc gtcccgtcgt accatatccc tggcagggtt gaaggcgtta gccgccacct
     1321 ccacggtcgg ggccagaatg gtgtagcccg ccgcctgccg ccagttcagt aacagcgccg
     1381 tcatcatgat ccccgcggcc agcgtggact tactgttttt cttggggata aggataaaca
     1441 cttccttgat atggcgaaca ccggtctgcg catcgtagga gccaaacagg gccgccacca
     1501 ggtcaaacac ccacggtgca caggactccc cgaatgtcgg gctacccggt gcatccacaa
     1561 tccgcagttg tttaaaaatc gccagtgcat gtgcagcctg gtccggataa atcggagccg
     1621 gaataatcga cagccccttt ttcaggcgct ctgcccagtc cgggcag
//
LOCUS       DAZUGK010000402         1655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZUGK010000402 DAZUGK010000000
VERSION     DAZUGK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.405"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHY5W_004886"
     CDS             <1..266
                     /locus_tag="ACHY5W_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4449594.1"
                     /translation="LWQTSRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVF
                     VRSIDDPDVALLVGLAREKGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            266..1222
                     /locus_tag="ACHY5W_004887"
     CDS             266..1222
                     /locus_tag="ACHY5W_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFS4449595.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            1233..>1655
                     /locus_tag="ACHY5W_004888"
     CDS             1233..>1655
                     /locus_tag="ACHY5W_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFS4449596.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLV"
BASE COUNT          371 a          430 c          468 g          386 t
ORIGIN      
        1 tcttatggca gacaagtcgc gacgttatcg cgttcctggc cgatgaattt aaagtggaca
       61 gcgtcaatcg tattaaaccc ggtattgctg aagcaacccg ggctgtatta cgtcgggtac
      121 cggaccatgt atttgtgcgt tctattgacg acccggacgt tgccttgctt gtagggcttg
      181 ctcgtgaaaa gggaatagtt gttacagaaa tggggggaac cctcggccag tatcgggctg
      241 taaccattat caagaaggta ctctgatgaa aaatcgtctt tctccctgga atctgggagc
      301 cacgctatac atgcctgcaa cacgggaaga tattgctgat gccgtcctgc acgggaaaat
      361 cccgggcttg cgctctctgg tgatttgcct ggaggatgct gtcagtgaag cagacatccc
      421 cgtcgccctc aaaaatctgg agcacctgct gcacgagctc agtaacagca tgagtagcct
      481 gggtaaaaat gactggcctc tggtgttcat tcgcccccgg catgccgaaa tgggcagatg
      541 gttaaaagcg cattatgatc tttccgctgt cgacgggttt gtgctgccta aatttaccct
      601 cagttcgtta gctgaatggt gggatatcat ggtcgggact cacctgtgca tgatgccaac
      661 gctggaaaca gaagatgtct ttgacgtggt tcagatgcgc gagctggcta cccgtctgga
      721 ggaacatccc tgccacgacc gcattattgc gcttcgaatc ggcggcaacg atcttatgaa
      781 cgttgtgtcg cttcgccgtc cccgggacct gacgctgtat gacagcccga tgggctacgt
      841 catcaaaatg ctcgtttcgg tcttcggccc gcgtgatttt gccctgaccg cgcctgtatg
      901 tgagcatatt gacgatcatg ccgttatggc cagagagctg gctcttgata tggcacacgg
      961 gcttgttggg aaaacggcca ttcacccggg tcagatagag gtcattcaaa acgcgctgat
     1021 ggtcactcag ggtgagcatt ctgacgctct gcggatcctg aactctaccc aggccgtgtt
     1081 taagtcgcag ggagcaatgt gtgaacccgc cacacatcgc cgctgggcgg ctggcattct
     1141 ggacagggct cgtttttatg ggttacagaa cgagcaaagc gctgatggaa tcagattact
     1201 taccgtgacc cagcatcatt aaggaaatca taatggggtt caggttcaga aagtcgatca
     1261 acattattcc tggcgttcgc ctcaacctga gtaacggtgc accgagcctg agtgtcgggc
     1321 cgagaggtgc ttccgtttct tttggtagcc gggggaccta tgccaatctg ggcttgcccg
     1381 gtaccgggct gagttaccgt acccggcttg accgggccgc gcgttccaga ggtgaaaacc
     1441 ggacggcaac cgacccgggg ctcagacagg cgcttgagca gaaagccgct gaactcatgt
     1501 cagcggtaac cgcaatccgt aatattcacg agctgacgcc ggatccaaaa acaggcatca
     1561 gctgggcaga gctggaagca gtatacctgc ataacagaac gtcgcctttt caggttccgg
     1621 caccggtgcg tccagaaaag ccagactacc ttgta
//
LOCUS       DAZUGK010000403         1654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZUGK010000403 DAZUGK010000000
VERSION     DAZUGK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.406"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..347
                     /gene="yciG"
                     /locus_tag="ACHY5W_004889"
     CDS             168..347
                     /gene="yciG"
                     /locus_tag="ACHY5W_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS4449597.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNSGNKSGKS"
     gene            433..933
                     /gene="yciF"
                     /locus_tag="ACHY5W_004890"
     CDS             433..933
                     /gene="yciF"
                     /locus_tag="ACHY5W_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4449598.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTQTLAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTELALNNVNK
                     KAENKA"
     gene            979..1485
                     /gene="yciE"
                     /locus_tag="ACHY5W_004891"
     CDS             979..1485
                     /gene="yciE"
                     /locus_tag="ACHY5W_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4449599.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1545..>1654)
                     /locus_tag="ACHY5W_004892"
     CDS             complement(1545..>1654)
                     /locus_tag="ACHY5W_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFS4449600.1"
                     /translation="MDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          549 a          338 c          351 g          416 t
ORIGIN      
        1 ttcctcaggc aattgttaat atatcaagaa cgttcctcat aatatatttt cccactttct
       61 tctcgttacg cttaatttga ctaattctca ttagcgacta attttaatga gtgtcgacac
      121 acaacactca tattaatgaa acaatgcaac gcaacgggag aaataacatg gccgaacatc
      181 gtggtagttc aggaaatttc gccgaagacc gtgagaaggc atccgacgca ggccgtaaag
      241 gcggtcagca tagcggcggt aattttaaaa atgatccgca acgcgcatct gaagcgggta
      301 aaaaaggcgg tcaacaaaac agtggtaata aatcaggtaa atcctgattc accgttaatt
      361 attaatatca gctgataaag ataattgcat ctgcgggccg tatggctcgc agtgattcca
      421 ctggagaaaa atatgaatat gaagaccatg gaagatgtat ttattcacct gctttcagat
      481 acctacagcg cagaaaaaca attaacccag acactggcaa aactcgcaag agcaacatca
      541 aacgaaaaat taagtcaggc ttttcatgcg cacctcgagg aaacacatgg acagattgaa
      601 cgtattgatc aagttgtgga atcagaatcg aatctgaaaa ttaagcgcat gaaatgtgtg
      661 gcaatggaag gtcttattga agaagctaat gaggtcatcg aaagtaccga gaaaaacgaa
      721 gtgcgtgatg ccgcactgat tgccgcagca cagaaagtcg agcattatga gattgccagt
      781 tacgggacat tagcgacgct ggctgaacaa ttaggttacc gtaaagcagc gaagcttctg
      841 aaagaaaccc tggaagaaga aaaggccacc gacatcaaac tgactgaact ggcccttaat
      901 aacgtaaata agaaagccga aaataaagcc tgaaatatga actttaactt ttagtcattt
      961 tataaagagg acattttcat gaatcgtatt gaacattatc atgactggtt acgtgacgcc
     1021 cacgcaatgg aaaagcaagc cgaatctatg cttgaatcca tggccagccg tatagataat
     1081 tatcctgaac tacgcgctcg tattgaacaa catcttagtg aaaccaaaaa ccagattgtt
     1141 caactggaaa ctattcttga tcgtaatgac atttcacgtt cagtcattaa agattccatg
     1201 agtaaaatgg ctgcgcttgg gcagtcaatc ggtggtatat tcccttctga tgaaatagtc
     1261 aaaggctcta ttagcggata tgtcttcgag caatttgaaa tcgcctgtta cacctctcta
     1321 ttagcagcag caaaaaatgc cggtgataca gcctcaattc cgatcatcga agcgatttta
     1381 aatgaggaaa agcaaatggc cgactggctg attcagcata ttccgcaaac aactgagaaa
     1441 tttttaattc gctctgaaac tgatggcgta gaagcgaaga aataataccc tttatgccat
     1501 gtccttattg accccgtata ttacggggtc gtttttgtgc ggaattaaaa acgatatcct
     1561 gctgagaaca taaacaccca cggatcgagg cgtacgctat catgttgctg tgcaccgccc
     1621 agtttataat tggcggtggt atcgatatcc atgt
//
LOCUS       DAZUGK010000404         1619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZUGK010000404 DAZUGK010000000
VERSION     DAZUGK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.407"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..532)
                     /locus_tag="ACHY5W_004893"
     CDS             complement(<1..532)
                     /locus_tag="ACHY5W_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449601.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFW"
     gene            complement(717..1406)
                     /locus_tag="ACHY5W_004894"
     CDS             complement(717..1406)
                     /locus_tag="ACHY5W_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS4449602.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(1421..1543)
                     /locus_tag="ACHY5W_004895"
     CDS             complement(1421..1543)
                     /locus_tag="ACHY5W_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFS4449603.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          510 a          406 c          278 g          425 t
ORIGIN      
        1 gccaaaaata tgccacaaaa gcaatcatca caatctcaaa aaaatgtttt tttataaatg
       61 gcttatcata gaagcatgat accgataaaa atcgcccata agatcttatc gaaattgtaa
      121 ccgccagcgc aatcgctgct gacagtagca aaagaggtac ctgaatcttc tgtctcaata
      181 tagaaaactc aataattgcc ggcacaaaca ataattccac agcaaaataa aggcgaaata
      241 catttagctc ttgcatagaa tgttttcttt tcactgcgaa aaagaataca acaccaatac
      301 cccaaccgat aagaaatata gcaatgacga taactgcaaa aaataaactt ctggcaacat
      361 catcaacacc tgcacctaca atccaccatg ggaagccgta gtaaaaagaa gtaccccatc
      421 catagaaata agcactcccc catccaaggc atcccatgta ggcaataaaa agtgaagaac
      481 tcctgagcag cgcaccatcc ttcataacca ccccaataca agatgataac attggcttac
      541 aactcataac aaaagcaatt caatgccgtc aagaggttac aggctaaaaa aactctatta
      601 catagcagcc agcatgttta ccgtacaagt acaactcagg gcataaaaaa aacccactcg
      661 gcagcgggtt tatacatttt ttacaacata ccaaatttgc atgaagtata tggcttttaa
      721 tccagttttg caatattttg ctgtaaaaat gctgcctttt gttttgaacg tgttctcgtc
      781 acaagcaata aagcatcact atcaagctgt agaaaaatgt gcttcattgc aacccagcgt
      841 tcagtaaatg tctcggacca gttttttgtt gtcactccca ccaatgatgc cagtgtctgg
      901 tattcatagg cctcacgccc tgcaagttcg ctcttcacat cctgtgcagc cagccagatt
      961 aacgtcttca ggcgatccag tgtcttacct gcaatttttc tgttacttaa caaatcttta
     1021 aactcgctcc atgcccattg cgttatggtg acctgatgcc cccatcgaac gctttcgctg
     1081 taacaccaaa gcaaccatgc tttctgatgt tcatcgagag acaaaaccgc gcggcgccat
     1141 gaagaggttg agaattcaac cgggctgacc aaagcaatgg atgaaccttt tgcgtacgac
     1201 tgcttaccgg aagtcggcgt attatccagc gtaatcatct tgccagttac cacatccaga
     1261 atgcgcggct tctttcgttt gtatgtacca gtatcaaatt gtgcatgctc ctgccaggct
     1321 tcgagctggc ctttcgttgc tccgttcaag tcagcagtag ctgccataag ttgctcacga
     1381 acatactgta aatattgggt attcatgcag taaatccttt ctatattttg gcataattct
     1441 tcaacattcg gtaatcgttc aaaaccgaat cggggaaacg acataagcac aggagccccc
     1501 agcgacagcg aaggagttct gatatataag actcagacat cattcattcc ccggttctcc
     1561 aatatctgtt tcactcatca tccataacta cctgtaattg cccccccttt ttttgtaac
//
LOCUS       DAZUGK010000405         1608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZUGK010000405 DAZUGK010000000
VERSION     DAZUGK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.408"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..221
                     /locus_tag="ACHY5W_004896"
     CDS             <1..221
                     /locus_tag="ACHY5W_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS4449604.1"
                     /translation="YQFDLLANGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNI
                     RFGLEFEKSASGKYNVDNAVNANFRYSF"
     gene            complement(269..886)
                     /gene="ureG"
                     /locus_tag="ACHY5W_004897"
     CDS             complement(269..886)
                     /gene="ureG"
                     /locus_tag="ACHY5W_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFS4449605.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(898..1572)
                     /locus_tag="ACHY5W_004898"
     CDS             complement(898..1572)
                     /locus_tag="ACHY5W_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFS4449606.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
BASE COUNT          338 a          442 c          444 g          384 t
ORIGIN      
        1 gttatcagtt tgacctgctg gctaacggtg aaaccgtact gcgtgatgca tccggcgaaa
       61 aacgcatcaa gggtgaaaaa gacggtcgta tgctcatgaa tgttggcctg aatgcggaaa
      121 ttcgcgacaa catccgcttc ggtctggagt ttgaaaaatc ggcatctggt aagtacaacg
      181 tggataacgc ggtgaacgcc aacttccgtt attccttctg ataaaaacaa ggggccgcct
      241 gccggccctg atacagagga tgttgtgatt acttacccag catgccctgc tcttcgataa
      301 aggcgatgat gttttgcaga ccttcaccac ttttcaggtt agtgaacccc catgggcgtt
      361 ctccgcgcat acgtaaagta tcacgctcca ttacctcgag ggaggcgccc acgtatggtg
      421 caaggtcagt tttattgatc acgaggaagt cggatttggt gatccccgga ccgcctttac
      481 gcgggatctt ttctccttct gccacatcta tgacatatat ggtaaggtcc gccagttcgg
      541 ggctgaaggt cgcactcagg ttgtcgccgc cgctttccac aaagatcaga tcgagattac
      601 caaatttctc actcagtgct tccacggctg ccagattcat tgatgcgtct tcacgaatgg
      661 ctgtatgagg gcagcccccc gtttcgacac cgacaatgcg ttccgcttcc agtgctccgg
      721 cctcagtgag aatgcgttga tcttctctgg tgtagatgtc gttggtgact acggctaatt
      781 gccaggtgtc acgcatggat ttgcagaggg cttccagaag cgcggtttta cctgagccga
      841 ccggaccgcc gacgccgaca cgcagcggtt gcttgattat gttcatttta ttttctccta
      901 agaacggaat aatcgggaat actgggtttc atgtcgcgcg gaagcgatag atgcaagtgg
      961 cgttgctgaa ccaattgcgt gatccggggt tgcaagggca ctgtccatgt tcgccgcata
     1021 gcgggcacac aggcgcataa ttaactgctg ggcagccagc tggccgtagg gtaccagcct
     1081 gacgcccgcc atcactgcgc tttcaatcca gctgtagcca aggctcaggg ccaggtctga
     1141 aacggatatt tgccagcgta cgccgagcca tgccataccg gttaactggc tttgctggca
     1201 tagtttgcgc cattcggccg ggcagtcggg ctgccagtcg accagaagtc tggtgaaggc
     1261 tgcaccgcgg ttacgttctt catcgcgtag ctcacgggtt tccctgcagg ccagcagata
     1321 agcggtccag cgcccggcac tgtcaggatc gcccgcttca caggcccggt atagccgggc
     1381 aagcagtggc aggtcgacgg caaaaaaact ctgttccatc tgcaactgct gccagtgttc
     1441 aaaggcggcg ctatcttcta cccagcctgc ttccactgcc cactccagtc cctgggacca
     1501 gctgtagcct ccaacaggga ggctgttact ggcaagctgc atcagccgca gacgtttttc
     1561 cggagtcggc attcagtggc tgtgctcctg atcgtgatta tgggcgtg
//
LOCUS       DAZUGK010000406         1603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZUGK010000406 DAZUGK010000000
VERSION     DAZUGK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.409"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..549
                     /locus_tag="ACHY5W_004899"
     CDS             <1..549
                     /locus_tag="ACHY5W_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4449607.1"
                     /translation="LVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHL
                     TGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPL
                     SSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRH
                     RHWHASAFTGIYNDLAAASACM"
     gene            546..1127
                     /locus_tag="ACHY5W_004900"
     CDS             546..1127
                     /locus_tag="ACHY5W_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4449608.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
BASE COUNT          360 a          391 c          498 g          354 t
ORIGIN      
        1 ctggtccaca gtcatcccgg tgggctgccc tggctgagcg aggctgaccg gcggctgcag
       61 ataaaaagcg cactgccctg gtggctggtc tgccggggtg acattcacaa attccgctgt
      121 gtgccacatc tgacaggacg gcgctttgag cacggggtga cggactgtta cacgctgttc
      181 cgggatgctt atcatctggc ggggactgaa atgccggatt ttcatcgcga ggatgactgg
      241 tggcgcaacg gtcagaacct ttacctggac aatatggcgg tcaccggctt ttaccgggtg
      301 cccctgtcct ctgcacaggc gggcgatatt ctgctgtgct gctttggtgc ttcggtaccg
      361 aaccatgccg ccatttactg cggcaacggt gagctgcttc accatctgcc tgaacaactg
      421 agtaaacggg agaggtattc cgaaaaatgg caacgacgaa cgcattctgt ctggcgtcac
      481 cgccactggc acgcatctgc cttcacgggg atttacaacg atttggccgc cgcctcagcc
      541 tgtatgtgaa cacggcagcg gaagccatcc gggcgctgtc gttacaggtg ccgggctttc
      601 gccgtcagat gaacgaaggc tggtaccaga tacgtattgc cggttatgac acggcaccgg
      661 aggcggtgta cgcccgtctt cacgaacagc tgggtgaggg aacggtcatc catattgtgc
      721 cgcgactggc cggggccgga aagggtggac tgcagattgt gctgggggcg gcagccatcg
      781 tgggctcttt cttcactgcc ggggcatcaa tggcgttatg gggttcagcc ctggcagccg
      841 gtggtttttc tgccaccacg atgctgtttt cacttggagc cagcatgatt ctgggcggtg
      901 tggcccagat gctggccccg aaggcaaaaa caccggatta ccgcgcaacg gataacggca
      961 gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac ccgatgccgg
     1021 tgccttacgg ggaaatgctg gttggctccc gccgtatatc ccaggacatc agcacccgtg
     1081 atgaaggcgg gggcggaacg gtcgtggttg tcgggcgaca gggataaaac ataaaaaaat
     1141 cccgcagtga tcgcggagct gcggggacag acaaatgaag atcaatgtta aggagttgtt
     1201 tttgttactc gggcaaaaaa acactaacgc agcgaaatta taagcgccac agtcagtgtg
     1261 tgaaaatgtg aagatattca gaatttttat gccattaccg gttttaacca acaggattat
     1321 cggtgggcat gaaagaaaac cccggtatct gctgataccg gggtttctct ttagcatggc
     1381 agaaatgtgt ttcatgcttt tcgggcgaag gatatccgac ttctgtacgg aatggcaagt
     1441 ggcggttaat ttattcaggg gaaggctgta tgggaaaagg tggcggtaag gcacacacgc
     1501 ctcgtgaggc gaaggataat ctcaaatcca cgcagatgat gagtgtgatt gatgcgattg
     1561 gtgagggacc gatagaaggt ccggtgaagg gactgcagag tat
//
LOCUS       DAZUGK010000407         1601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZUGK010000407 DAZUGK010000000
VERSION     DAZUGK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.410"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..436)
                     /locus_tag="ACHY5W_004901"
     CDS             complement(77..436)
                     /locus_tag="ACHY5W_004901"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4449609.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1185..>1601
                     /locus_tag="ACHY5W_004902"
     CDS             1185..>1601
                     /locus_tag="ACHY5W_004902"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4449610.1"
                     /translation="MQDFNICCRRDNHYPEHKLIPLSELFDVNYGLNLELNKLEKDSS
                     GINFVSRTSKNNGVSARVKLKDGISPLPAGVLTVAAGGSVMESFVQDSPFYSGRDLYW
                     LRPKVELTLEEKLYYCSCIRRNRHKYSYGRQANRTLK"
BASE COUNT          476 a          306 c          332 g          487 t
ORIGIN      
        1 gaggataaga ggtgaatacc tcttatcctt aagtaaatat taaccaggta actgtgaata
       61 atttagatgt tgggcgttat gaaagtaata aaactcccca tataatgata gcaactaaaa
      121 tcgtaatcaa cactgcggct gaacccatat cttttgctct gcctgataat tcgtgatgtt
      181 ctgaaccaat tctgtcaaca actgcttcaa ttgcactgtt aattatttca acaataatta
      241 ccatcattgc cgaaccaatg agtaatactc gagtaacaga gtcaacatcc agccaaaagg
      301 atattatcag tgctgacaga accagaatgc actcctgacg aaaggctgct tcatagcgcc
      361 atgccgcaca taagcctttc catgaataac ccgctgcttt aataattctg gtaacaccag
      421 tggtcgtatt agccataaat acctcttatt taattatatg tctgaatatg cattgtaaaa
      481 tatcagttaa cattaacagg attttgtaat aagccacatt tattgtttcg ggaattaaaa
      541 aacataacga atttttgcct cattctgcat ttcattgaaa tagtatgttt ctcatcgaaa
      601 gcaagtttgt caatataaat ttgtaacaga aatgactttc cggggataaa caaaactgtt
      661 catggcaatg gctttctctg tcgccaacat ggatacatcg ttccggataa agcactgatg
      721 ccattgactg acgcagacta tcaggtcatc tgatgctttc ttttccttca ccggtattga
      781 tgatatgcct tatgaagggg gacttattcc tgtaacccaa tcacaggcgc tgtgattact
      841 ggtttatgat tgatgctgat ttgagcagat atgcacagca gttgtggatt caggggatat
      901 tttctgccag atattcatac cagattgatg acctctttgc tcagatgctc ttcgttctga
      961 gccagccagg attttgtggc cacatacatt gttcgccgat ggggaatcga gacaggcgat
     1021 tggctgtgat gcgattttgc cgctaactga ggatgagtcc gcccctcccg gggaattcct
     1081 gtaagttaag ccgtacctgt tgattagcac taaactgggt aatgaattta tctgccagta
     1141 tgaatgatgc cggggacggt tttgaactcc tgatgtcggg taatatgcag gattttaata
     1201 tttgttgtcg tcgggataat cattatcctg aacataaact gataccttta tcagagttat
     1261 ttgacgttaa ttatggactg aatcttgagc tgaataaact ggagaaagat tcttcaggaa
     1321 ttaattttgt ttcaagaacg tccaaaaata atggcgtctc cgccagggtt aaattaaaag
     1381 atggtattag tccattgcca gcaggtgtgt taactgtagc agcaggaggt tctgttatgg
     1441 aatcgtttgt acaggattca ccattttata gtggaagaga tctttattgg ttacgtccta
     1501 aagttgaact gactctggaa gagaaattgt actactgctc ttgtataaga agaaacagac
     1561 acaaatacag ctacgggcgt caggcaaacc gaacgctgaa g
//
LOCUS       DAZUGK010000408         1599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZUGK010000408 DAZUGK010000000
VERSION     DAZUGK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.411"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..233
                     /gene="dicB"
                     /locus_tag="ACHY5W_004903"
     CDS             45..233
                     /gene="dicB"
                     /locus_tag="ACHY5W_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS4449611.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            230..421
                     /locus_tag="ACHY5W_004904"
     CDS             230..421
                     /locus_tag="ACHY5W_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS4449612.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            515..>1599
                     /locus_tag="ACHY5W_004905"
     CDS             515..>1599
                     /locus_tag="ACHY5W_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS4449613.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETGKYTSVVDLARA
                     KANRQHTENPAEKIPPVTAAIHREYKQTWKTLDRELAYYLWPGDADAGNIDGSILRWA
                     KNEVIARDREDWKRISASMRKQPDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPET"
BASE COUNT          484 a          382 c          366 g          367 t
ORIGIN      
        1 gcaccaggca ccgcatcaca aaattcattg ttgaggacgc gataatggaa aagttatcat
       61 gtaatgccag cacgtctgaa cttcgtttcg aaattggcgt tatcactgga gacaaaacat
      121 ttattgaaga cgccattaag cagagaaaac tcgagcagga cctgttaaat gaagtatgca
      181 ttccttcaat gctggctcgt ctggacctgc tgcaaaaagg atataaacaa tgaatacaac
      241 atttgcactc gttctgacag tttatcttgt ttccggcgaa tctcttgagc tggtgactgg
      301 cttatacggt tcaatgaaag aatgcatggc tgcagcagca gaacaaaaaa ttcccggtaa
      361 ctgttatccg gtagataaaa ctactcacac taataataac gaaataccgg caggacttta
      421 aaacagcacc gtaattaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      481 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      541 cattccggac gaacaggcaa taaaagaaga gggcgcagtt gctgtagcca ctgccattga
      601 agccggtgat gaacgccgcg cccgtgccaa atttacctgg caattcctgg agcaatatcc
      661 tgctgctcag gactgcgctt ataaatttct tgtctgcgaa gataaacccg gcatgccccg
      721 ccctgctatc gactcctggg ataccgaata tatgcaggaa aaccgctggg atgaggaatc
      781 cgcttccttt attccggtcg aaccagaatc cgatcctatt aacgtcaatt ttgacaagct
      841 gtcccttgaa gtacagaacg cggtcctggt taagttcggt acatgtgaaa acatcaccgt
      901 tgatatggcg attgacgcgc aggaattact gcaagaagac gtggctacct ttgacgggca
      961 tatcgttgaa gcactgatga aaacgcctga aattaacgct atgtatccgg aacgcaaact
     1021 gttcgctatc ggatgggtta aacacaaatg taagccgggt accaaatggc ccgaaattca
     1081 ggctgaatta cgtaactgga aaaaacggca ggacgcagag cgcaaagaga ctggaaaata
     1141 cacgtctgtt gttgatcttg cccgcgccaa agccaaccga cagcacactg aaaacccagc
     1201 agaaaaaatc cctcctgtaa ctgccgccat tcatcgcgaa tacaagcaga catggaaaac
     1261 cctggacagg gagctggcct actatctctg gcctggtgat gcggatgccg gaaacattga
     1321 cggcagcatc cttcgctggg ctaaaaatga agttatcgcc agagatcgcg aagactggaa
     1381 gcgcatctcc gcatcaatgc gcaaacaacc tgatgcgctt cgctacagcc gccagactat
     1441 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1501 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa gaaatcagag
     1561 cgcaactgat actctctcgt cgccagtacc agaaactga
//
LOCUS       DAZUGK010000409         1598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZUGK010000409 DAZUGK010000000
VERSION     DAZUGK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.412"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(83..508)
                     /locus_tag="ACHY5W_004906"
     CDS             complement(83..508)
                     /locus_tag="ACHY5W_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449614.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPTAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSTYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(492..734)
                     /locus_tag="ACHY5W_004907"
     CDS             complement(492..734)
                     /locus_tag="ACHY5W_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS4449615.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            1126..1464
                     /locus_tag="ACHY5W_004908"
     CDS             1126..1464
                     /locus_tag="ACHY5W_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS4449616.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
BASE COUNT          431 a          396 c          332 g          439 t
ORIGIN      
        1 gcgttagcca tactcacctc ctctgatacc gaatcttttt actcacgaat tgccggaagc
       61 gattcgatat ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac
      121 ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca
      181 gtcgctcccg cgtctccacc agctcccgaa aataagttga actgtggctg cgcattctgg
      241 ccaccagcaa aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa
      301 ctgcatcagg ggtgtctttc tctatccagc ggaaaatttt ctgggtattg cgagccaggg
      361 cttccggatg gctgtcgtca tacagttccg ggaacgtcat acccagttcg aaatacgctt
      421 tggtaatctt cgcagtcggt actttttcgc cgtccggatg cgcccaggca ttcatcgcca
      481 tgcggatgtg ttcatgcttg attttcatga atcccccctt ggttagaagg cggattatga
      541 tcagaatcgg gaatgacaac cgtcggtatg tgtaactcat atttgagcgc cccggcagtg
      601 actgcctgaa ttagcaacgc ccatttccac ggaacgtctt ccccccacat gctgactgtg
      661 gtttttgacg ttcctagagc ggcggctgtt ttaacaactc cgccaaaata gcctaatact
      721 tctgattttt tcatgagtcg ctccataaaa ctgaacgcca aaagtttaat aatcaaaacc
      781 aaagaaagtc aagaaacaaa accatctgtg ttttaaaatc aaaacatgaa caagcaaaca
      841 atatctgaac gcataaccca acgtatgcat gcgctaaact tgaaaggcaa agaccttgtc
      901 aatgccactg gcgcatcaaa aggctccgta agtcaatgga tgaacggtgg aggagcgccg
      961 tcctcgcgtt acataagttc actggcaaag atattgaaag taaacgaaaa ttggcttctt
     1021 aatggaggag agttaaatac aggtgattcg cttgatctat ctttaccgcc gataaaaacg
     1081 gttccgctac tatcacttca gcaggcagca agctggagtg attatatgaa aaattcctca
     1141 ataacctctt gtgtgcagct tgtcggagaa atcccggcca atacctttgc tgttgttcta
     1201 gagagtgaca gtatgtcgac atctggtggt ggagtttcca tcccaaatgg ttcaacagtt
     1261 tttgttgatc ccgatcgaat cgtacaacca ggaaatattg tccttgcctt acccaaaggg
     1321 accacaacgc ctgtcattcg caaactggag atagaagggc cggatattct tttagtcccc
     1381 acgaatcctc gctacccttc aattatgctg gatgatctat cttgcatatt gggagtatgc
     1441 tttaaaattc aacaagatat ttaaccaacc tcatctattt gattaactgt atgccatcgt
     1501 agtgatggcg taacagctgc ctgcttaaaa tgttttgata aaaaaacatt gaccttaaat
     1561 gttcattttt ctaaacttca ttcattccct caccccgc
//
LOCUS       DAZUGK010000410         1586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZUGK010000410 DAZUGK010000000
VERSION     DAZUGK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.413"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..1202
                     /locus_tag="ACHY5W_004909"
     CDS             9..1202
                     /locus_tag="ACHY5W_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4449617.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
BASE COUNT          332 a          410 c          459 g          385 t
ORIGIN      
        1 acattaaaat gtcacctggg atcgaagata cgccccaaaa accactgtcc tgctggccac
       61 ttgcgttcag tgccgggctt ctcggtatcg ggcagaacgg tctgctggtt gtgctccctg
      121 ttctggtcat acagacaaat ctgagtctgt ctgtatgggc tgccctgctg atgcttggct
      181 caatgctgtt tctgccatcg tccccgtggt ggggaaagca aatttcccgt actggcagta
      241 aacctgtggt gttgtgggct ctgggaggat atggcataag ctttaccctg ctagggctgg
      301 gaagcgtgct gatggctacc agcgccataa caacagcggt gggactggga atattaatca
      361 tcgcccggat cgcctacggg ctgaccgtgt cggcaatggt gccagcctgt caggtctggg
      421 cattgcagag agcaggagaa gggaatcgca tggccgctct ggcaaccatc agctccggcc
      481 tgagttgcgg caggctattc gggccgctgt gcgcggcagc aatgctggcc attcatcctc
      541 tggcgccact ggggctgctg atggcagccc cagtgctggc gctgctgatg ctgctgcggt
      601 tgcccggcac accaccacag cccacaccgg agcgcaagag cgtcagtctg aagcgggatt
      661 gtctgcctta tctgctttgc gcaatattac tggctgcggc ggtgagcatg atgcagcttg
      721 gactttcgcc tgcccttact cgccagttcg ccacggatac caccgccatt agccaacagg
      781 tggcctggct gttgggtctg tccgcagtag ctgcgcttat cgcgcagttc ggggtactcc
      841 gtccgcagcg cctgactccg gtggccctgc tcctgagtgc cggagtgttg atgagtggtg
      901 gtctggctat catgctctcc gaacagctat ggttgtttta cccgggctgt gcagtgctgt
      961 catttggagc agctctggca acacccgctt atcaacttct actgaatgat aagctggctg
     1021 atggcgcagg cgcgggctgg ctcgctacca gtcacacact tggctatgga ctgtgcgctt
     1081 tgctggtgcc gctggtatcg aaaacaggtg tcgcaatcgc gctgattatg gcggcattat
     1141 ttgcaactat attatttacc attgtgtctg tatttatctg gcattaccgt gctatcaaat
     1201 aaagatatga tcatcacctt accggagtaa ctcagaggat atgagagaga acctgcgtct
     1261 gatgaaagca tgtcaggcag taacactggc aaaaatgcac cgcatggtac agggtatgtt
     1321 cggtataaaa ccggccggtg tcacttaagt tgcattgaag tcatgctggt tgtgagtgca
     1381 acatcacatg gctctgtccg gatgcctgca gacaatgaga acagtgcgat tactcattgg
     1441 gaatgagatg aacgcatccc caacattaga tctggggcac cgtgataaat aaaggggaaa
     1501 atcagagagg tgtcgatcct cgcagatttc cggcatcata gtgcccaata cgggatgcat
     1561 aaaactctct cagagtggcg ttgaca
//
LOCUS       DAZUGK010000411         1585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZUGK010000411 DAZUGK010000000
VERSION     DAZUGK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.414"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..444)
                     /locus_tag="ACHY5W_004910"
     CDS             complement(<1..444)
                     /locus_tag="ACHY5W_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4449618.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQ"
     gene            complement(434..>1585)
                     /locus_tag="ACHY5W_004911"
     CDS             complement(434..>1585)
                     /locus_tag="ACHY5W_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449619.1"
                     /translation="IFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNRFLRAGVE
                     VDRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHIFEPVEDGQTRGANQFY
                     SVMERLKMLDSLQATQLQSAIVKAMYAATIESDLDTEKAFEYIAGAPQGQKDNPLINI
                     LDKFSTWYDTNSVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGV
                     SYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIR
                     PPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGED
                     YQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETTPRET"
BASE COUNT          353 a          510 c          390 g          332 t
ORIGIN      
        1 ctgacggagg cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg
       61 actgtcaatg tccagcagta tgccccgcac ctggctatct gccattgcct gctgaagaca
      121 ggcgacaatg ccgtcatagc cagtcattcc ggaaaatggc cgcatccccc ccagccggtg
      181 caccagcgtg ccggtcaccg gcagtaccgc aataccgttc accacccggt aaacacgggc
      241 cggtcgttta cctccggcca tgtactcgtc cgtttcagcc agcattccgg gagcatcaag
      301 ctgtacctgc tgttgtggta ccgaaagact tgctgccccc atctcgcgcc cgagcgcgca
      361 aaagaaaacc cgcgcatagg cgggctccag aagcagcggt tcattgaatg ctgctgcaat
      421 aatgtgtgaa agattacgtc tcacgtggtg ttgtctcctc ttccggcctg cgactctccg
      481 ctatctgctg ctgatacgcc tgcgctatcc acaccggacg tgagagtccg gctttttccc
      541 gctctgcaga ttccctgacc tgctggcgga aaatgtcctg ataatcctcg cccatcagcg
      601 ccagctcttt ctcatacgtg ctcagtccgg cctcaatgcg catcactgat tcctgaacct
      661 ccttgagccc gtcaatggcc attcttccgg ctccaatcca ctcagcccgt gaccaggctg
      721 atcgcgcctg ataaaaatca aaacgtgccc gtggcggacg aataatcccc cgaagaagtg
      781 cctcttccag ccagcaggaa aacatctgcg tggccagccg ggacgcaata aattttcgcc
      841 gccccataaa atagcgccac gactcattgg cggatgcgcg ggcacttgaa taactgacct
      901 tcgagtaatc acgggacaac tgttcgtagg aaacgccaag accggcggcg atataccgca
      961 gcagcgcctg ttcaagcgcc gaaaatccat tgtctgaatc ctgcgcggtc tgaagtttca
     1021 gatcatcacc ggggaaaagg tgcggaattt tgacaccgcc cagcgtcacg ctattcgtgt
     1081 cataccaggt ggagaactta tccagaatat taataagcgg attatccttc tgcccctgcg
     1141 gcgcaccggc gatatattca aaggcctttt cggtatcaag gtcactttca atcgtcgctg
     1201 catacatcgc cttcactatg gccgactgaa gctgtgttgc ctgcagggaa tcgagcatct
     1261 tcagccgttc cattacgctg taaaactgat tggccccacg ggtctgcccg tcctccaccg
     1321 gctcgaaaat atgcagcatg gccggacgcc cggtgggaag ttcacgcggg atccgttccc
     1381 atcgtccact cccagagaac ggaaaatcat cctcacagat atggtacgca acggcacgac
     1441 catatcgatc gacctccaca ccggcccgca gaaaacggtt ccccataccg tgtcctggcg
     1501 tgtccacccg tttcggactc acggctttaa aacgcgtacg aaacagttgc gtgctctccg
     1561 tatcccagac cggctgcaca aagat
//
LOCUS       DAZUGK010000412         1565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZUGK010000412 DAZUGK010000000
VERSION     DAZUGK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.415"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            180..473
                     /gene="iss"
                     /locus_tag="ACHY5W_004912"
     CDS             180..473
                     /gene="iss"
                     /locus_tag="ACHY5W_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFS4449620.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(564..746)
                     /gene="rzoD"
                     /locus_tag="ACHY5W_004913"
     CDS             complement(564..746)
                     /gene="rzoD"
                     /locus_tag="ACHY5W_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFS4449621.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(963..1460)
                     /gene="rrrD"
                     /locus_tag="ACHY5W_004914"
     CDS             complement(963..1460)
                     /gene="rrrD"
                     /locus_tag="ACHY5W_004914"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFS4449622.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1460..>1565)
                     /gene="essD"
                     /locus_tag="ACHY5W_004915"
     CDS             complement(1460..>1565)
                     /gene="essD"
                     /locus_tag="ACHY5W_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFS4449623.1"
                     /translation="AIGVLGSLVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          392 a          377 c          347 g          449 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 ccctcctcca ataggctgag catccaccta tatagtttta attttcatca atccatttaa
      121 ctatcgttta attgttgtca cataggattc tgccgttttt aacaatgcag gataataaga
      181 tgaaaaaaat gttgttttct gccgctctgg caatgcttat tacaggatgt gctcaacaga
      241 cgtttactgt tggaaacaaa ccgacagcag taacaccaaa ggaaaccatc acccatcatt
      301 tcttcgtttc gggaattgga caggagaaaa ctgttgatgc agccaaaatt tgtggcggcg
      361 cagaaaatgt tgttaaaaca gaaacccagc aaacattcgt aaatggattt ctcggtttta
      421 ttactttagg catttatact ccgctggaag cgcgtgtgta ttgctcacaa taattgcatg
      481 agttgcccat cgatatgggc agctctatct gcactgctca ttaatatact tctgggttcc
      541 ttccagttgt ttttgcatag tgatcagcct ctctctgagg gtgaaataat cccgttcagc
      601 ggtgtctgcc agtcgggggg aggctgcatt atccacgccg gaggcggtgg tggcttcacg
      661 cactgactga cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa catcatcacg
      721 cagagcatca ttttcagctt tcgcatcagc taactccttc gtgtattttg catcgagcgc
      781 agcaacatca cgctgacgca tctgcatgtc agtaattgcc gcgttcgcca gcttcagttc
      841 tctggcattt ttgtcgcgct gggctttgta ggtaatggcg ttatcacggt aatgattaac
      901 agcccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg cggttactct
      961 gttcattgct gaccccacaa acagatttca cgctcaatct cacgacgagt catgagacct
     1021 ttccattgct taccgccagc atatgtccag cgacgtagct gatcacatgc gcctttgata
     1081 tcgccctggt ttattttgcg aagaagcgtc gatgttctga aattgccagc acccacgttg
     1141 taaacgaatg agtaaagagc gccgcgcatt gtttccggta tatcgacttt gatgtacggg
     1201 ttaatttgtc tggcgaccgt ggcaaggtct ttattcagga gggctttgca ttctgctttg
     1261 gtatacgttt taccgagcat gatgtctttt cctgtatgcc cgtgacatac agtccataca
     1321 ccaacaatat ctttgtatgg tatgtagctg acaccttcca gaccatcgtt accacttggg
     1381 ccagtgatta acactgatgc tatagcaatt gctccgccac caatagcagc agcaacggct
     1441 tttcgtaatg atggaggcat tattcacctc tcgcagcctt gcgcttatct tctttaatct
     1501 tgaaataaag gtttgtcagg tacgtcagca ggccaaatac caggctaccc agcacaccta
     1561 ttgct
//
LOCUS       DAZUGK010000413         1555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZUGK010000413 DAZUGK010000000
VERSION     DAZUGK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.416"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..1439)
                     /gene="ccp"
                     /locus_tag="ACHY5W_004916"
     CDS             complement(42..1439)
                     /gene="ccp"
                     /locus_tag="ACHY5W_004916"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFS4449624.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDEKA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          398 a          420 c          325 g          412 t
ORIGIN      
        1 taaaaatcgc ccaaacattc ctgttcgggc gatcaaatta attattgttt atcctgcata
       61 tacggcgtgt acaccccgtt cagactgtgc aggaaagcta cgatatcatc cacatcctcc
      121 tgcggcagct ctttgcctac ctgatagcgc aacatcagtt tcaccgcccc gtccagcgtc
      181 ggcacgtcac cgcgatggaa gtacggtgcc gttaaagcaa cgttacgtaa accgggtact
      241 ttctgacgca atttatcacg ctcttcttta gtcacattca tacgaccaat atccgccgcc
      301 gtaatttcac caaagttaaa gtcttttttc agccccaacg gttcgaagga acgtccgccg
      361 agaataatac caccatgaca agttgcacat ttattatctt taaataattg atagcctttt
      421 ttctgttgcg ccgtcagcgc tttctcatcc ccacgcaacc atttatcaaa tggggaatcc
      481 ggcgtaatta atgttttctc aaattcagca atggcatcag taatattttc gccactgaaa
      541 ccttgcggat agacttcgag gaactgcgct ttaagctgcg gatctttttc cagcttagca
      601 ataatttcgt cccaggattt cgacgccatt tcaatcgggt tcaacggcgg tccaccagcc
      661 tgatcctgca atgttgccgc acgaccatcc cagaactgct caacgttaaa tactgagtta
      721 aataccgtcg gcgcgttaat cggcccaact gcgccaccaa caccaattga tgtttttctg
      781 ccatcgacgc cccccgcatt caacgcatgg caatgagcgc atgaaatggt gctatcagcc
      841 gataaacggg gatcgtgata cagcgcaaag cccaacgcca ctttttgcgc atcggtaggc
      901 agtttttgcg ggatgggctg caccggttca ttgcgatgct ccggagcagt atcattgctg
      961 gcgtaatatt ccgcgcgctg ttttgcaatc caggccagta tttccgcccg ctcttcatca
     1021 ctcaccttac ccgcccagtg aagcgcggta taacgcgttg gtggcatagt ttcatactgc
     1081 atcacccatt caatcttatt cagatcgctt tgcgaaacgg gtttatcagc cagcagtgcc
     1141 gcacgcacgg cctcgaggtt aaaagattta tatccaagct taatgtcgta atccatcaac
     1201 tgtttcgcgc caggaatata ataataggcg ggtaattctg ccgaaggcgt gtggcaatag
     1261 tcgcatcctt tttcgcggag aaagcctaaa accttattat tttcactgac agcagatgcc
     1321 tgaacatcgg cctgtttact gcgtttatta tcgtggtacc acacataacc agataaccct
     1381 aaatagcaaa tcgcgacgcc agccaggccg atcgcggtaa tacgtgagac cattttcatt
     1441 attcttccct cacggtggtg agttattgtg acccagaaaa ttttccagaa tgctgcaaac
     1501 gtctctgcga gacaaagcca tcataaaaaa cagggagata cactttttga tagga
//
LOCUS       DAZUGK010000414         1543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZUGK010000414 DAZUGK010000000
VERSION     DAZUGK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.417"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(225..1100)
                     /locus_tag="ACHY5W_004917"
     CDS             complement(225..1100)
                     /locus_tag="ACHY5W_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFS4449625.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDERKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYKKIYLSGDGSAGLPLLKCGDEFLS
                     PTDIVDRIVQHNLHEIDDIRLTSCNSANILKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KSSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1241..1510)
                     /locus_tag="ACHY5W_004918"
     CDS             complement(1241..1510)
                     /locus_tag="ACHY5W_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4449626.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          397 a          350 c          294 g          502 t
ORIGIN      
        1 aatgtaataa ttacatacta ttagtgattc tcatgcatcg taagcggctc gccagaaccg
       61 tattgatatt tactgagcgc tcagatcaac tttccatggc aacagatcgc gttcccggtt
      121 tgccggccag tcctggatat gctcccgccg acaatattaa tggccaggaa atgtttaaaa
      181 caatgattct gtttcctaac catcaagtgt atatgctggc tcaattactc aatatctatg
      241 ggttgagttc tacccaaagt ggctctgtag tcgcttcttc ttacagtatg ttcaggtgtt
      301 gcaggcacag ttgtggagcg tagatgtgtt gttggtttac catgctctgg tacataaaaa
      361 acgccagtgc catggtaacc tgatatagaa acgttaattc cgcgacgttc aaactcggca
      421 tagacgtgtt ctgctaaaga actcttttga ccaaataatg ccctagccaa ccagccgtta
      481 ttcatatttg cggatttctc tatttcatca ggagagaagt ctttgttttt taatatgttg
      541 gctgagttac aggatgttaa tctgatatca tcaatctcat gaagattatg ttgaacaatg
      601 cggtcgacaa tatctgtcgg cgataaaaat tcatctccac acttaagaag tggtagacca
      661 gcagaaccat ccccagacag ataaattttt ttgtattttc ctggtgttaa atcatctggc
      721 aggatggttt tcatctctgt cgctgtaata cttatagctg cagcagcaac aacatcgcta
      781 ttacttgatt gtagatggct tttatttgct ccaggatatg taaactccat tttcctttca
      841 tcaaaatctt gttttgtagc atcattcagg aataataaat catacggttt tttccccgtt
      901 gtgactcttg aaacattttg gccaagaata ttaagagtat agtcattaac ggatttcatt
      961 cctaaaaaaa caagtaatcc ttctttctct tgcgcttttt cgattgttct ccctgctaaa
     1021 gttattggga tggattgaga ttgttttgtc ggaactgata cgctggcgct aataggtagt
     1081 agggttctta tgctaaacat acaacctctt tcttaatttt cgaacctgtt taggattctg
     1141 tgtaaattca aaataaacct gtctgaacat tcctaacaaa catccacctg acatgacaac
     1201 aaaaaccgga gccggactcc ggtttttgtg aagctgtcgg ctatttcatt ccgccaatat
     1261 tttcccacgt cccgtcagca cgcaggattt gcagcggtct taccacgcac tgtatctgct
     1321 ttttatccgc atccagtatc accacctgtg tgattaccct gtcctgctcc gggataatgc
     1381 cattctcatc tgactccaga atgtctgccg gtcccagtcg cagctgtgct gtaagtaact
     1441 ccccgtgttc acggtcatca tgctttccgc aaccacacag acgctgcata agttttttta
     1501 gtatgttcat gtcattctcc tgttctgcct gtatcactgc cca
//
LOCUS       DAZUGK010000415         1537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZUGK010000415 DAZUGK010000000
VERSION     DAZUGK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.418"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1381
                     /locus_tag="ACHY5W_004919"
     CDS             <1..1381
                     /locus_tag="ACHY5W_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFS4449627.1"
                     /translation="HLRRNVSTRSSFSAETYRDEFDRFIKRFPIIGSSTHSIINSIGK
                     GALLDYVIIDEASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYN
                     CEKYSLLDSVCMLFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDSGEASL
                     SLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPADFVKS
                     TVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGND
                     VFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSH
                     FKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDF
                     VVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKL
                     GAFLSGLTG"
BASE COUNT          429 a          289 c          372 g          447 t
ORIGIN      
        1 acatctgcga agaaatgttt caacaagaag ttcattctcg gcagagacat acagggatga
       61 atttgaccgt tttataaagc gttttccgat tattggtagc agtacgcatt cgattattaa
      121 ctctatcggc aagggggccc ttcttgatta tgttattatt gatgaagcct cgcagcagga
      181 tattgttccg ggcattctgg gactgggatg tgcccgtaat gtaatcgttg taggggatcg
      241 taagcaactt ccacatgttc ctgtattact cccgaacagc ccatctccac cggcagaata
      301 ctataattgt gagaaatata gtctacttga ttcggtttgt atgttgttca ggaacatggt
      361 gccagtaacc cttcttaaag agcattatcg ttgtcatcct aaaatcattc aattttgtaa
      421 taagcagttt tatgataatg ctctgatacc gttaacggtt gattccggtg aagcctcatt
      481 atctctggtc atcacggcaa aaggaaacca tacccgtaat ttttcaaatc tcagggagct
      541 tgaatctctg gaaggtcatt actgggatga agaaagtagc aggggataca ttgccccata
      601 taatgcacag gttaatctgg ctgaaaaagt gctgcctgct gattttgtga aatcgacggt
      661 gcataaattt cagggacggg agtgcgatga aattgtgttt tccactgtgc tggataagaa
      721 gcgcagcagc cagcacagtc ggaacatcgc gtttgttgat aatcctgaac tggtgaatgt
      781 cgctgtttca agggcccgaa ataaatttac gctggtgact ggtaatgatg tatttgaacg
      841 ccacgctgga catattgcgg cgctgattcg ttacatcaaa tactacgctg atgacggaga
      901 gattttcgag agcccggtca tatcagcatt tgatttgctg tacagtgagt atgacaaatc
      961 actggaacga ctgaatagta gattgaattc aaatgattca cactttaagt cagaacagat
     1021 tgtggcctgc ctgttgcggg atatcctgtc gcaggatagt tatcggagta tgatgtttca
     1081 ttcacaaata gcgctgaatc agttggtgtt gctggagcgg ggggatttta cacaccgtga
     1141 acagttattc atgcgtaacc gggccagttg tgactttgtt gtttactata aggtcgggaa
     1201 gactccatta ggggttattg aagtggatgg aggttatcat ctgaccagtg ttcaggctga
     1261 acgtgatgaa cttaaaaaca gtatactgaa gaaatgcggc ctgccattgc taagattacg
     1321 tactatagac agtgatatag aagggaagtt gggagcgttt ctgtcaggcc tgacaggcta
     1381 aaatctgaat aaaaaatatt gcagcgggag gtctcaaacc tgatttggtc atgtgccaca
     1441 cacaattgta cgaaccccat ggacacaggc ttctttgttt tggttacatc aacgtacgta
     1501 ttcctgatac atataaaaat aagtgctatt gctcaaa
//
LOCUS       DAZUGK010000416         1524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZUGK010000416 DAZUGK010000000
VERSION     DAZUGK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.419"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..206
                     /locus_tag="ACHY5W_004920"
     tRNA            112..206
                     /locus_tag="ACHY5W_004920"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:146..148,aa:Sec,seq:tca)
     gene            369..>1524
                     /locus_tag="ACHY5W_004921"
     CDS             369..>1524
                     /locus_tag="ACHY5W_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449628.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAKAREKRDTARKQVANGIDPVEERKAQKLAQKLSTENSFEA
                     ICREWHTNKADRWTVAYREEIIKTFEQDVFPFIGKRPISEIKPLELLEVLRRIEKRGA
                     LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRD
                     LEAYTGSLITKNATKIVMLTGVRTQEMRFATWEEVDLEKGIWEIPAERMKMRRPHIVP
                     LSIQVVDLFKQLKPITGHYPFIFIGRNNHSKPISKESVSQVIELLGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIEMQLAHVDKNSIRGTYNHALYLEKRREMIQWYSDY"
BASE COUNT          465 a          330 c          351 g          378 t
ORIGIN      
        1 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
       61 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
      121 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
      181 ggttcgactc ctgtgatctt ccgccaaaca tcatccagcc agttccgaga tactccataa
      241 aatcctttat attcaacacg atgaataata aacttgtccg taatagtccg agatcatctc
      301 cctgaatccg cgaaaaatat gtatagtaat gtgtatagat tttttatacg tagttttcag
      361 ttatacacat ggctctaacc gatatacaga tcaaacgtgc aaaaccacaa gacaaaccat
      421 acacattgaa cgatggacaa ggtctgtcat tgcttatcaa tcccgatggc tcgaaaggct
      481 ggcgtttccg tttccggttt gcagggaaag cgcggttaat gtcatttggc agctacgatt
      541 tagtaagcct cgcaaaagca cgtgagaaac gcgatacggc tcgtaagcag gtagcaaacg
      601 gcatagaccc agtggaagaa cgtaaagccc aaaagctggc acaaaaactc tcaacagaga
      661 actcattcga agccatatgt cgagaatggc acactaacaa agcggaccgc tggacagtag
      721 cctatcgcga agaaatcatt aaaacatttg aacaagatgt attcccgttc attggtaaac
      781 gccctatcag tgaaatcaaa ccgttagaac tacttgaagt attgcgacga atagagaagc
      841 gtggagcact agagaagacc agaaaagtgc ggcaaagatg cggtgaggtc tatcgctatg
      901 cgatcataac tggccgcgct gaatacaatc cagcgcctga tttagctatc gctctagccg
      961 ttcctaagca aaaacaccat ccttttttat ctgctgaaga gttacctcat tttattcggg
     1021 accttgaagc gtataccggt agccttatca ccaaaaatgc tacaaagata gtcatgttaa
     1081 ctggtgtaag aacacaggag atgcgatttg ctacgtggga agaggttgac cttgagaaag
     1141 gtatatggga gatcccagct gaacggatga aaatgcgtag gcctcatatt gttcctttat
     1201 ctatccaggt agttgacctt tttaaacagc ttaaacctat tacaggccat tatcctttca
     1261 tctttattgg caggaacaat cacagcaaac cgatctcgaa ggaaagcgtg tcgcaagtga
     1321 ttgagttact tggttacaaa ggccgtgcta caggtcacgg ttttagacat acaatgtcga
     1381 caattctgca cgagcaaggg tttgatagtg cttggattga gatgcagtta gcacacgtgg
     1441 acaaaaatag cattcgaggt acttataatc atgctctata tcttgagaag cgtagagaaa
     1501 tgatacaatg gtattctgat tatc
//
LOCUS       DAZUGK010000417         1516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZUGK010000417 DAZUGK010000000
VERSION     DAZUGK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.420"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..63)
                     /locus_tag="ACHY5W_004922"
     CDS             complement(<1..63)
                     /locus_tag="ACHY5W_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449629.1"
                     /translation="MGIKVQRLSLWMINTVLLLLP"
     gene            complement(116..520)
                     /gene="ycgX"
                     /locus_tag="ACHY5W_004923"
     CDS             complement(116..520)
                     /gene="ycgX"
                     /locus_tag="ACHY5W_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFS4449630.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(741..1472)
                     /gene="bluR"
                     /locus_tag="ACHY5W_004924"
     CDS             complement(741..1472)
                     /gene="bluR"
                     /locus_tag="ACHY5W_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFS4449631.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          398 a          339 c          328 g          451 t
ORIGIN      
        1 gggtagcaat aaaaggacag tatttatcat ccatagggat agtctctgta cttttattcc
       61 cattatgcta atgccttact gaattatgaa gcatttctta agcatccaac tttagctaga
      121 ttaatggttt attattttct acatcttcaa tatataaaag cgtattatca atggcgtagt
      181 aactgcgttt gttatgatta acatcagtaa cccaacggaa aacgcccgcg tctgccagtg
      241 ttgaacagta ttcccgaaat gtagattttc cgcaaatatg aagcaatgcg gcctctttta
      301 ttttagcagg gttcttggtc gtactaactt ttaacaggtt cctggttcct cttaataaca
      361 aaaccgtgtc atcgtgagta ataattctga tgttatccgt agccagataa taaatgtaat
      421 gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc tcttttctca
      481 cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata acctcttgtt
      541 agttgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg tctgtccttt
      601 ggctgtgata tgtacagagt gtgatagagg gattacctga attctcccgg tgagcatttt
      661 gcaacggacc agctccggta caaatgctgt tgtgggttca gattataaca ttctgtctaa
      721 ggggcgggat aaaggtgaaa ttagggggca tgaaagatga ctttataacc ttgctcaccc
      781 cagtgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca gacaatcaat
      841 gtttgcgtcg ggaacagttc cgggcgcggc gattcaatgg gatcagcaag aacagaaatg
      901 tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct ctcttcaaca
      961 ttccatgcca tcagaatggc ttctttaccg ggcttacggc gcatttcgaa aagcgaggct
     1021 gctgcgtatt caattagtgc gccgtcaaac atactgctca taatgcggga ggtgttgtga
     1081 tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg atctactggg
     1141 tactctcgac ccagtgaaat aattctcgcg cgtagtttgg caggattagc catacgaaga
     1201 attgacatca tctcttcttg caagcggctc cagtcatctt ccgtatcctg gctggtggtt
     1261 tccagtaatg ctttaacttt gcctacaggg acgccattac ttatccaacg cttgatctct
     1321 tcgatacgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact gcgctgtggt
     1381 tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat cccgcaacgt
     1441 tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca caaatctccg
     1501 tcgcctgtac acgacc
//
LOCUS       DAZUGK010000418         1516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZUGK010000418 DAZUGK010000000
VERSION     DAZUGK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.421"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..405
                     /locus_tag="ACHY5W_004925"
     CDS             40..405
                     /locus_tag="ACHY5W_004925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449632.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            405..>1516
                     /locus_tag="ACHY5W_004926"
     CDS             405..>1516
                     /locus_tag="ACHY5W_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFS4449633.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTG"
BASE COUNT          353 a          364 c          449 g          350 t
ORIGIN      
        1 tataaaaaca gacaatatca ttgtctttca ggtagttata tgtcccgttc agctaaaccc
       61 cgtaaacgaa aacctgcccc tcaaagaagc aaacttcccc gctatgtcgt gaagcttcac
      121 gacgatgact tctttgacga agaagacgca gaagctctgc gctttgataa ttttgacgat
      181 gccgttgagt gctgcgcaga cctgaatatt cccttctttg tggatgccgg aaacaaaaag
      241 ctggtcttct ggtttgtacg tgttgatgac gaagggtatc ctgaaatagc ccgctgcacg
      301 gagcgggagt ttgcgaccat tcttgccggt atcagcgccg gcggcatgta ctgcccggag
      361 tgtggcacgg ttcactggcc ggacggagtc cccccgccct tctgatgctt ccccgttttg
      421 ccgacatttt tcagcaggga aaccgctggc ttaactggct ggagaaacaa ccggaaggtt
      481 cagtgcgtcc ggtagtcatt gagtctgtga caaaaatcat ggcctgcggg accacgctga
      541 tggggtacac acagtggtgc tgttcatctc cggactgcag ccacataaaa aaggtctgct
      601 tccggtgtaa aagtcgctcc tgcccgcact gcggagtgaa ggctggcgca cagtggatac
      661 agtatctgct gagtctggtt cccgactgtc cgtggcagca tattgtgttc acacttccct
      721 gccagtactg gtccctggtg ttccacaacc ggaggttact ggcagagatg agccgcattg
      781 ctgcggatgt gatacaggaa atctgccgcc aggcagatgt ggtgccgggg atattcacgg
      841 tgatccacac atggggacgt gaccaggagt ggcatccgca cattcacctg tcgacaacga
      901 ccggcggcgt gacatcagac cacacctgga aaaaccttca tttttacgcc cgtaaggtga
      961 tgagtatgtg gcgttaccgg ataacgcggt tactgtcacg gaaatatccg gacctggtga
     1021 taccggatgc gctggcagca gaaggaagca gtaaacggga ctggaatcgc ttcctggaca
     1081 gtcattaccg gcggggctgg aatgtcaacg tatcccgggt gatggataac gccacacatg
     1141 tggcggtgta cttcggctct tacctgaaaa aaccgccggt gccgatgagc cgtctggagc
     1201 actatgctgg tcaggatgaa attggtctgc gttacaacag tcaccggaca aaacgggaag
     1261 aatacctggt gatgagtggt gatgagttta tggaaaggtt ctcctggcat gtggcggata
     1321 aggggttccg tatggtgagg tactacggtt tcctgagtcc ggtgaagcgc cggttactgg
     1381 aagatgttgt gtacgtcata acggagacgg tgagaaagac ggcgatgcaa atcaggtgga
     1441 gagggatgta tcagcggtta ctgaaggttg acccgctgaa gtgcatcctg tgcggaggtc
     1501 agatgcgttt tacggg
//
LOCUS       DAZUGK010000419         1461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZUGK010000419 DAZUGK010000000
VERSION     DAZUGK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.422"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1461)
                     /gene="ehxA"
                     /locus_tag="ACHY5W_004927"
     CDS             complement(<1..>1461)
                     /gene="ehxA"
                     /locus_tag="ACHY5W_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFS4449634.1"
                     /translation="VGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTER
                     GVAIFAPQLDKLLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALD
                     ELLRKQREGEDISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPR
                     LSSVGGKLQNLPDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQV
                     LGNVGKAVSQYILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLE
                     SYSERFKKLNYEGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPI
                     SMLVSALTGTISGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFL
                     EDSLSLLADFSRQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGG
                     LLEAQPKEFTQQVFDPQKGTIDLSTGNVSSVLTFITP"
BASE COUNT          394 a          356 c          272 g          439 t
ORIGIN      
        1 ggtgttataa atgtcaaaac acttgataca ttacctgttg aaaggtctat ggtccctttt
       61 tgaggatcaa acacttgttg tgtaaactct ttcggttgag cctctaaaag ccctccattt
      121 tccagataat taatatatgc cttaccactc tgactgcgat cagcattacg ggttatgcct
      181 gcaagttcac cgatcttctc atcccaatgt tgctgggtta ttgcgactgc tctttctact
      241 gcatgctgac gagaaaaatc agcaagcaaa gacagagagt cttctaaaaa cgcagcatgt
      301 cttgcgtcat agccattctc aaaataattt ttgccatgct ccttttccca ttcattgatc
      361 cgagcagcga atttctctgc aacgtgctca aacatagcct gttttgatgc ttccagaatg
      421 ccagatatcg taccggttaa tgcactcacc agcatgctta tcggggcccc tatgagggat
      481 gcactggagg ctgcactaac tcccgcagat acagaactca ggacagtatt tattgttgtc
      541 agggctgcat ctatagctcc ggtttcttta tgaaaggctg cgagtaaagc atccccttca
      601 taattcaatt ttttaaatcg ttcagaatat gattcaagct gcttagctcg ctcaaattta
      661 tctgcagcag ccaggaaaga aagaggactg atagccagca taacagccga tgtgatcaga
      721 cccgcacttg cagctgttgt cgataacccc tgtgccattc tctgagccag aatatattgc
      781 gaaacagctt taccaacatt tccaagaacc tgagttgtca gttcgatacc cgctgcagct
      841 tttgttcctg tatgtgcgtc actgtttccc agaataaagc ttgctgatac agcagaaaga
      901 attccggaga caacatccag cccatccccc agggagccca ggtctggtaa attttgtaat
      961 ttcccaccaa cagaacttaa gcgaggttta ctggataaaa atgagccaag ctggttaagc
     1021 tgctcagaaa atgaatcaac ggtactgttt atacttgata ctgtatctac aagctgatta
     1081 ataagttcaa tactactttt ggcaatatca ttctgactta tatcctctcc ttcccgttgt
     1141 tttctcagca attcatcaag agccatgcct gataaagcaa tccccgtaaa attctgtagt
     1201 gctgagagaa ctgttccggc ttttcccaga ttattaccta cattttcagc ggttcttcct
     1261 attttactcc caactttctg atacttctgc agaagtttgt caagttgtgg tgcgaatatt
     1321 gcaacaccac gttcagttaa tcctacaact ttttctgctg caccaaaaaa ctggtttgca
     1381 atcgctgtat catctcgttc agtacggtgt atttctattc caagctcatc agcagctttg
     1441 accaactcat taatacccac g
//
LOCUS       DAZUGK010000420         1461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZUGK010000420 DAZUGK010000000
VERSION     DAZUGK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.423"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            234..455
                     /locus_tag="ACHY5W_004928"
     CDS             234..455
                     /locus_tag="ACHY5W_004928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449635.1"
                     /translation="MQDIDYNAECKISYEFPEYTSKKNEAAELVIDFKSFNGKGASNA
                     KINRIKSEIMKTLESVKIIAYERHRNKDQ"
     gene            complement(817..1389)
                     /locus_tag="ACHY5W_004929"
     CDS             complement(817..1389)
                     /locus_tag="ACHY5W_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFS4449636.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTVIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
BASE COUNT          419 a          345 c          321 g          376 t
ORIGIN      
        1 tttccttaaa gacatctctg aaagtcatcg tgagaatgaa ttctatttca ataaaattat
       61 tgatgttgat tttcatccag atgaaaatgc cactttccct cagggcatgg agtggttaga
      121 aaaaaaatat agaagaactc aaactcaaag ggactcttgg cgattcaatt ttctttagaa
      181 aaaaaagcat tcacccaagt ctaaaaatag caaaactagt cgcaagctac actatgcagg
      241 atatagatta taatgctgag tgtaaaatat catatgaatt tcccgaatat acatctaaaa
      301 aaaatgaagc cgcagagtta gtaattgact tcaaatcttt caatggtaaa ggtgcgtcca
      361 acgctaaaat caacaggata aaatcagaaa tcatgaaaac acttgaaagc gtaaaaatta
      421 ttgcttacga acgccatcgt aataaagacc agtaatatac aataacgccg ccgacaggcg
      481 gtgttattat acatttgcta taagcataca attaatactt taacttcccg cacgatcatt
      541 cttagtaaat cgtgcagaaa agcagcaatt ttcaatcaaa accatcacaa cgaactcaag
      601 ggctaaacaa ataaatcact acgccggcgc gcagtgcttt ccccgcctcg cccgcccgct
      661 ttgcagggcg gttttaatgc agttgcactg acacgctcag gccgcgccgg gaatggcgcg
      721 gtctgcagaa aatgaggcag gaaaacgcat gcaaagccat gcaccttatc gatgcatggc
      781 ttttttcagt aaaaacaggc ggattttcgg ggaattttac acagactgac gtgatgccag
      841 ttgcgcactt ttacgcgaaa aaatcatgtt ctgcgcaggg gtgaattttt cacggctgtc
      901 atccaccgaa gccgcgtcag gcctgaatcc gatggtcgtt aaaatgtcgc tatcctgtgc
      961 ggaataatta attttttccc ccttcgcaag ccaggacaga agggcttcac gcagggcatc
     1021 tgtggcacgc tgtatggcac agtttcgggc aatgaccgtc agctcactgt agcccatcag
     1081 ctccggtgcc agtgccgccg ccagtgctgt gccgtgctgc tgcataaaat cattcagccg
     1141 gtcgcggatg ctgatgtgct gaacggcttc atgcgaacga atataacgac cggcggcctg
     1201 attcacctgc cattttctga cttcgataat attgcgtaat tcgtccaggc gactgacgtt
     1261 tctgccttct cctgacagaa gccgcaggta ttcctgttcg gccgctgcca gctcattttt
     1321 gcgttgcagc catgctgctt tgttattctg acaggtgtca aaggcctgct gtaaggctgt
     1381 gctttccatc gttatctctt tctcatcatg ctgaagaata aaaatacggt gtgcggcgac
     1441 ggccggggtt aaccggcagc c
//
LOCUS       DAZUGK010000421         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZUGK010000421 DAZUGK010000000
VERSION     DAZUGK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.424"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..444)
                     /locus_tag="ACHY5W_004930"
     CDS             complement(<1..444)
                     /locus_tag="ACHY5W_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS4449637.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQ"
     gene            complement(434..>1459)
                     /locus_tag="ACHY5W_004931"
     CDS             complement(434..>1459)
                     /locus_tag="ACHY5W_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449638.1"
                     /translation="VEVDRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHI
                     FEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEY
                     IAGAPQEQKDNPLINILEKFSSWYDTNSVTLGGVKIPHLFPGDDLKLQTAQDSDNGFS
                     ALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQ
                     MFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIE
                     AGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYQQQIAESRRPEE
                     ETTPRET"
BASE COUNT          323 a          470 c          361 g          305 t
ORIGIN      
        1 ctgacggagg cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg
       61 actgtcaatg tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca
      121 ggcgacaatg ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg
      181 caccagcgtg ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc
      241 cggtcgttta cctccggcca tgtactcgtc cgtttcagcc agcattccgg gagcatcaag
      301 ctgtacctgc tgctgtggta ccgaaagact tgctgccccc atctcgcgcc cgagcgcgca
      361 aaagaaaacc cgcgcatagg cgggctccag aagcagcggt tcattgaatg ccgcggcaat
      421 aatgtgtgaa agattacgtc tcacggggtg ttgtctcctc ttccggcctg cgactctccg
      481 ctatctgctg ctgatacgcc tgcgctatcc acaccggacg tgagagtccg gctttttgcc
      541 gctctgcaga ttccctgacc tgctggcgga aaatgtcctg ataatcctcg cccatcagcg
      601 ccagctcttt ctcatacgtg ctcagtccgg cctcaatgcg catcaccgat tcctgaacct
      661 ccttgagccc gtcaatggcc attcttccgg caccaatcca ctctgcccgt gaccaggctg
      721 atcgcgcctg ataaaaatca aaacgcgccc gtggcggacg gataatcccc cgaagaagtg
      781 cctcttccag ccagcaggaa aacatctgcg tggccagccg ggccgcaata aattttcgcc
      841 gccccataaa atagcgccac gactcattgg cagaggccct ggcacttgaa taactgacct
      901 tcgagtaatc acgggacaac tgttcgtagg aaacgccaag accggcggcg atataccgca
      961 gcagcgcctg ttcaagcgcc gaaaatccat tgtctgaatc ctgcgcagtc tgtagtttca
     1021 gatcatcacc agggaaaagg tgcggaattt tgactccgcc cagcgtcacg ctattcgtgt
     1081 cataccagct ggagaacttc tccagaatat taataagcgg attatccttc tgctcctgtg
     1141 gcgcgccggc gatatattca aaggcctttt cggtatcaag ttcactttca atcgtcgctg
     1201 catacatggc tttcacaatg gccgactgaa gctgtgttgc ctgcagggaa tcgagcatct
     1261 tcagccgttc cattacgctg taaaactgat tggccccacg ggtctgcccg tcctccaccg
     1321 gctcgaaaat atgcagcatg gccggacgcc cggtgggaag ttcacgcggg atccgttccc
     1381 atcgtccact cccagagaac ggaaaatcat cctcacagat atggtacgca acggcacggc
     1441 catatcgatc gacctccac
//
LOCUS       DAZUGK010000422         1443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZUGK010000422 DAZUGK010000000
VERSION     DAZUGK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.425"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..491
                     /gene="rfaY"
                     /locus_tag="ACHY5W_004932"
     CDS             <1..491
                     /gene="rfaY"
                     /locus_tag="ACHY5W_004932"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFS4449639.1"
                     /translation="NERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIF
                     NYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSK
                     DGVRIIDLSGKSCTAERKARDRLAMECHLGIANEIKDYGYYSVIYRTKLRKFIKKLKG
                     KA"
     gene            517..>1443
                     /locus_tag="ACHY5W_004933"
     CDS             517..>1443
                     /locus_tag="ACHY5W_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4449640.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDIYFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQNYFNEAYQASCWNDVAFIPATNEKQYQV"
BASE COUNT          435 a          250 c          307 g          451 t
ORIGIN      
        1 ggaatgaacg ttttttaaaa tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg
       61 aaaccgatcg tgtcagaagt gcaggtctta cttttcctaa tgatttttat tttctggctg
      121 agcgtaaaat tttcaactat gccagtgttt ttatcatgct tatcgagtat gtcgagggcg
      181 tagagctcaa tgatatgccg attattccag aaaatgttaa agcagaaatt aaggcttcaa
      241 tggaaaaatt gcatgcctta aatatgctat cgggcgaccc acaccggggt aactttatcg
      301 ttagtaaaga tggtgtcagg ataattgacc tatccggtaa aagctgtacg gcagagcgta
      361 aagctcgtga tcgtctggcc atggaatgtc acctgggtat tgccaacgaa ataaaagatt
      421 atggctacta ttcagtaatc tacagaacca aactacgcaa atttattaaa aaattaaaag
      481 gcaaagcgta aaccacatag tcaaaacgga accaacatgg atttattagc tgagagtatt
      541 actgaagtcg ctgtctctgg ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac
      601 ggtattgacc gtaacttttt atttggtgcg gcagtatcta tgcaatcagt tgttatgcat
      661 aacccggacc ttgcggttaa gtttcatctc tttaccgact acattgatga agattatcta
      721 caacgtgtta atgcttttac cagcaaaaat gctaacgttg aagtaagaat ttataaagtc
      781 tccaatgcct ttattgatat cttccccagc ctgaaacagt ggtcttatgc aacattcttc
      841 cgtttagttg cgttccagta tctgagtgaa actattgaaa atctgttata tatcgatgct
      901 gatgtcatct gtaaaggctc attagctgga ttacttgata tttattttga tggggataag
      961 tttgcagctg ttattaaaga tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct
     1021 atagagggac ttccagggaa ttatttcaac gccggtgtag tatatctgca gcttgaagca
     1081 tgggcgaaaa atgattttat gaataaagcc attgctatgc tggcaagtga cccgcagcac
     1141 acgaagtata aatgccttga tcaggatatt ttaaatattc tgttctttgg tcattgtatt
     1201 tttattagcg gcgattatga ttgcttttat ggcattgact atgagttaaa gaataaaagc
     1261 gatgaagatt ataaaaagac cattaccgat gatactaagc tgattcatta tgttggcgta
     1321 acgaagccct ggaacgactg gacgaattat ccctgccaga actattttaa tgaggcttat
     1381 caggcttctt gctggaatga tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa
     1441 gtg
//
LOCUS       DAZUGK010000423         1438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZUGK010000423 DAZUGK010000000
VERSION     DAZUGK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.426"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1159
                     /locus_tag="ACHY5W_004934"
     CDS             <1..1159
                     /locus_tag="ACHY5W_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFS4449641.1"
                     /translation="TTGPNDENVNNKPSSDMPNRIKPVVSDSQIGIYTTDSNTLKDGL
                     CSQAHIGTQPYRPGADSPITVRSFLQITLSNNNYKSLEHLQTRAENYLRYRKAEENIL
                     RSTVEGLTNPESPVFKQTAWMGHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSP
                     FYGSRGLKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKF
                     RADNRIDAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYA
                     AEHFGKTWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKY
                     VNTLDIFTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT          440 a          319 c          327 g          352 t
ORIGIN      
        1 cacaactggg ccaaatgatg aaaacgtaaa taataaaccg tcaagtgata tgccgaacag
       61 aatcaaacca gttgtaagcg attcacaaat cggtatatat acaactgaca gtaatactct
      121 gaaggatgga ttgtgtagcc aagcccacat aggaactcaa ccttatcggc caggtgctga
      181 ctcgccgata actgtgagat cattcctaca aatcacactt tccaacaata attacaaatc
      241 attagagcat ctgcaaacac gagcagagaa ttatctgagg tatcgcaaag cagaagagaa
      301 tattctgcgt tcgaccgttg aggggctaac caatccagaa tctcctgtat tcaaacaaac
      361 agcatggatg gggcatctgg agcgtggcct ttggaaaacg gaaacacgct gggatggaaa
      421 cgaccgcgag cagctaggca aagaggctct tggctcagag gagccaaaac ctggttcacc
      481 attttacggt agtcgtggac taaaattatc cgacagtgcg cattctgcct tctcaatgat
      541 gctgtgcggt tctgaaggtc cattcacaaa agaacaagca ctctctggtt tcgagcttgc
      601 tcaaactggc caagtgctgg ctggaaggct aaagattcag gagcgagtta aattccgcgc
      661 agacaaccgc attgatgctc agcgtaacgg tacacactca acccgcacac caacaggcat
      721 ggatctttct caagatatcg gcacaattat gcgtgataaa gctgggctac cggtcatgag
      781 cggaacatcc ggttcctcct ctgatgccac tcttgcaaca cgttacgctg ccgagcattt
      841 tggtaagaca tgggccgctc caggcttaag ccaagcagaa ggatgcaaag ctatctcgga
      901 tctgtcacat cactattttc gtgcagaagg ctccagtcca ccacaatcaa tggcaacggg
      961 aataaataag gttcgttatg atgcaggaat ggaagagaaa tatgtaaata cactggatat
     1021 atttacacat agctacccag aaatttatgc tggggttgca ttgacgattg ctggggctgg
     1081 cggtaatgat gagcaggcaa tgtataatgt tacgcaagaa gccgctcgta ttctgcacga
     1141 agctgaaact aaagattgaa catgttctag tgaagaggcc taccccagaa gaatatattc
     1201 cacattcaag gcattattga tgaattttat aatcaatgcg aaaaaactgg cattgattgt
     1261 atacagattc tcctgaagga gaatctgttc tgtcccttct gccaagcagt gttacgcaac
     1321 gatcaatctt tcgcgttcct attaatcaat gagttagagt tgattagtga cctgagacag
     1381 agcattagcg caaggtgatt ttttgtcctc ttgcgctaat tttttgtcat caaacata
//
LOCUS       DAZUGK010000424         1437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZUGK010000424 DAZUGK010000000
VERSION     DAZUGK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.427"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1345
                     /locus_tag="ACHY5W_004935"
     CDS             <1..1345
                     /locus_tag="ACHY5W_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS4449642.1"
                     /translation="KVEVESSVEREGPFYFLFSDKDGEKYGRANKLSGLNKALAAGAT
                     EITKEEYFARKNGTYSGSQQNTGASDTIAQPEPVKVTADEVNKIMQAANISQPDADKL
                     LAVSRGEFVAGISDPNDPKWVKEIETRDSVNQNQQETEQNDQKAEQNSPNALQNEPET
                     KQSEPVVQQEPEKVCTACGQSGGGNCPDCGAVMGNATYQETFDEENQVEAQENDPEEM
                     EGAEHPHKENTGGNQHHASDSETGEAADPLIKVNGHHNLTSTSRAGIHLMIDIETMGK
                     NPNAAIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDVIKWWLKQSREAQSAIL
                     TDEIPLDDALLQLREFIAENSGEFFVQVWGNGANFDNVILRRSYERQGIPCPWHYHND
                     RDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
BASE COUNT          442 a          338 c          366 g          291 t
ORIGIN      
        1 caaagtggaa gtcgaatcat ctgtagagcg tgaggggccg ttctacttcc tcttcagcga
       61 caaggatggc gaaaaatacg gtcgtgcaaa caaactttct ggtctgaata aggcgctggc
      121 tgcaggggct actgaaatca cgaaagaaga atattttgcc cgcaaaaacg gtacatactc
      181 aggttcacaa caaaatactg gtgcatctga cacgatcgca caaccggagc cggtaaaagt
      241 taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc ctgacgccga
      301 taagttgctt gctgtatcac gtggtgaatt tgttgcaggg attagcgacc cgaatgatcc
      361 gaaatgggtg aaggagattg aaacccgcga ttcagtgaat cagaaccagc aagaaacgga
      421 acagaacgac cagaaagcgg aacaaaacag cccaaatgcg ttacaaaacg agccagaaac
      481 gaaacaatcc gaaccagtag tgcaacagga accggaaaaa gtctgcaccg cctgcggtca
      541 gagcggtggc ggcaactgcc ccgattgtgg cgctgtcatg ggcaacgcaa cataccagga
      601 aacattcgat gaagagaatc aggttgaagc tcaggagaat gatccggagg aaatggaagg
      661 cgctgaacat ccacacaagg agaacactgg cggcaatcag catcacgcca gcgatagtga
      721 aactggcgag gcggcagatc ccttaattaa ggtgaatggt catcacaatc tcacatccac
      781 cagcagagcg gggattcatc tgatgatcga cattgaaacc atgggaaaaa atcccaatgc
      841 cgcgattatc tcaataggcg caatattttt cgatccacaa accggagata tgggaccgga
      901 atttagcaag accatcgatc tggatactgc tggcggagtt attgatcgtg acgtcattaa
      961 atggtggctg aagcaatcac gtgaagcgca gtctgccatt ctgaccgatg aaatcccgtt
     1021 agatgatgca ctactgcaat tgcgggaatt tatcgccgaa aactccggtg aattttttgt
     1081 tcaggtctgg ggaaatggag ccaacttcga caacgtgatt ttacgccgtt catacgaacg
     1141 gcaggggatc ccctgcccct ggcactacca caacgatcgc gatgtacgca caatcgttga
     1201 gctagggaaa gccatagact tcgatgccag aacagctatc ccattcgaag gtgagcgcca
     1261 caatgcactt gatgacgccc gttaccaggc aaaatacgtt tcagctatct ggcaaaaact
     1321 gatcccgagt caggctgatt tttaatgttc aaccatcgcc ggttgtgact ggtattctgc
     1381 aaccggccct catctgatgt aagagataaa agcgatgagc gaagtaatca tgattgt
//
LOCUS       DAZUGK010000425         1428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZUGK010000425 DAZUGK010000000
VERSION     DAZUGK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.428"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..913)
                     /locus_tag="ACHY5W_004936"
                     /pseudo
     CDS             complement(1..913)
                     /locus_tag="ACHY5W_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
     gene            complement(910..>1428)
                     /locus_tag="ACHY5W_004937"
     CDS             complement(910..>1428)
                     /locus_tag="ACHY5W_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4449643.1"
                     /translation="RIDILSKLLAFIDELTGSVEREHHELFADALPFASEVVLFSKVA
                     SEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQSLIQTSLYDLGELYGVSYSH
                     FRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTIAHKYGYSSSSHFSAEVKSR
                     LGKTPRELCKKL"
BASE COUNT          427 a          311 c          236 g          454 t
ORIGIN      
        1 gctttggaat atcaagcgta gctactaact tctcgataaa atcaacctga gatactgttc
       61 ctttaacaag taaactgttg gtatcaggat atgcaataat ttgtatatcg tcagtctctg
      121 gcgctcctgc tgtcgatatt attttctcta cttgtaggtc atcatcttct tcagcaccag
      181 gaaaaggagg catacgtgat acgtttttta gttgttgacg acttgaaatt tcagaaagta
      241 cgttcacccc tgtgctttgc ttaatgttat tatttaacaa cgtcgatagt acttttgcca
      301 tgccaggaat aactatcttt tcgccacgta actcataagt ccggtcatta acgaatgtgt
      361 tgaccagatg aattattcct actttatttc gaccgatctc aatgccatcg ctgttttgct
      421 ccattaattt cgcagcatta acgaccaggt caacataaac cggtggcccc gatacataga
      481 aagtaccatt gccgccccct ttaatttcat aacgagagtt atacagaccc gattttttta
      541 agaaattatt aaactcattc gtcgatacct ttcccagatt aatcaatgca ttacgcatct
      601 ctgatgagtc atagatataa atagcttagc catcatcata ccaaataagc cccatctgtg
      661 ccgacaggcg ttcaatcaag gcatagggtt ctgttaaatc aaaatttcca ctgatctgtt
      721 tcttcatggc aagcttacta actattgtag gcttacccgc atatgatgaa accgtttcaa
      781 aaaaactacg tagattttct ttatttgcta cgtatccatt cgctgctgcg gcatatgcac
      841 caggagtcaa taatccatta aaaatgcata agactgaaat taatataata gtaatgcgta
      901 attttatttt cataactttt tacatagttc tctcggtgtt tttcctaacc ggctttttac
      961 ttcggctgaa aagtgtgatg aggacgagta accatattta tgcgcaatag ttgtcatatc
     1021 actgtttcct tcaatgattt ccaacacggc tctggcaact ctccagccac ataattccgt
     1081 tttaacttta ccacccaatg cataactaca taaacggcga aaatgtgaat acgatacgcc
     1141 gtaaagctca ccaagatcat agagtgaagt ctgtatgagc gattgcgata ataaataacg
     1201 aaccagttta tagcattctg tttttcttag atgctgaaac aggatttcat taatattatc
     1261 gctgctcaga taagtggcta aaaaccaggc ttcacttgca actttactga atagcacaac
     1321 ttctgatgca aagggcaagg catcagcaaa caattcatga tgttcccttt ccacactccc
     1381 cgttaattca tctataaaag ctaataattt actcaggata tcaatgcg
//
LOCUS       DAZUGK010000426         1386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZUGK010000426 DAZUGK010000000
VERSION     DAZUGK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.429"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(26..1183)
                     /gene="acrE"
                     /locus_tag="ACHY5W_004938"
     CDS             complement(26..1183)
                     /gene="acrE"
                     /locus_tag="ACHY5W_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFS4449644.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          335 a          339 c          346 g          366 t
ORIGIN      
        1 aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt atctgcgggg gtatcggtag
       61 tggctttaac ctgctctccc ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc
      121 cagatttcag tccttcacta atcaaccatt tatcgccaat cgcctgactg gcaacgacag
      181 ggcgcgcttc aacctgactt ttatcgttaa caatcagcac ggttgcatca ccacgcggtg
      241 tgcggctaac gccttgttgc gggataagaa tggcgtcagg ttggacgcct tcatcaatcc
      301 gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg gttagggaag acagcacgta
      361 gggttatgga gccggtgctt tcatcaacgg tcacatcgga gaattgcagc gtacctttca
      421 ggggataggt ttgaccgttt tccatgacca actctacgtt gctggtggcg ttttccttat
      481 gcaaatttcc ttgctctacg gactgcttca gcctcataaa atcgttgctg gattgggtca
      541 catcaacata gataggatcg agctgctgga cagttgccag ttcagtcgtt tgcccattag
      601 tgacaagagc gccttcggtc acagtcgatt tgccgatacg tccgctaatt ggcgcagtca
      661 ctttggtata agcaagattg atgcgagcgc tttcgactgt ggcttttgcg gcaatcacgg
      721 cggcatcggc ctggcgagca tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt
      781 tcgtacccac gagcggaacg taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt
      841 cacttttcgc cagttcgcct ttcgcgctgt cataatttgc ctgataggtc gcgggatcga
      901 tctggtacag ggactggcct gcttgcacat cgctgccttc agtgaaattg cgattcagta
      961 cgatcccgct aacctgtggg cgaacttcgg ctatacgata agcattggtg cggcctggta
     1021 attcagtctt aacttctaac ggggccgttt ttacaatatg aacggtaacc tgcggttcac
     1081 cgacgtgagc tttctcttct cccttatcgt tacaaccggc gattaaagcc gcggagatca
     1141 gaataaagga gggcaggagg aaaaacctgg catgtttcgt cattactatt cctcaaaaaa
     1201 ccaaaagcgc gttatttacc caaaaaggca acgcgttaac tcgcagaaag aaaaatacag
     1261 ttcgctatcc tacaaattat cattcgtcga tgtaaggaat agttatgaat acaggcgtct
     1321 caaggcacat aaacacaaaa aaagattaat attctactgt tttattttga cgcgggttga
     1381 aagagg
//
LOCUS       DAZUGK010000427         1385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZUGK010000427 DAZUGK010000000
VERSION     DAZUGK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.430"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..831
                     /locus_tag="ACHY5W_004939"
     CDS             <1..831
                     /locus_tag="ACHY5W_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS4449645.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
     gene            883..1266
                     /gene="gpFI"
                     /locus_tag="ACHY5W_004940"
     CDS             883..1266
                     /gene="gpFI"
                     /locus_tag="ACHY5W_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS4449646.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            1259..>1385
                     /locus_tag="ACHY5W_004941"
     CDS             1259..>1385
                     /locus_tag="ACHY5W_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS4449647.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGV"
BASE COUNT          310 a          317 c          448 g          310 t
ORIGIN      
        1 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac
       61 cagcaggcgg ttgagcgtct tcccggtgaa gatccggctc aactgaacga cccggcctac
      121 cgtcgtctgc gtatcatcac cgataacctc aaacaggaag agcatgccat tgtccaggtg
      181 gaagaaatgc aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggatcag
      241 tttgatactg tcgaggtgga ttttggacgc tctgaaggaa ataacattga gcaggccgac
      301 ggtaaaaaat ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc
      361 tactgcgatc aggccagcgg tcttgtgaat attgccatta tggacggtac tgtctggcgt
      421 ctgctgaatg gttttaagct gttccgcgaa aaactggata cccgtcgcgg ctcaaattca
      481 caactcgaaa cggcagtgaa agacctgggg gcggtggtgt ctttcaaagg gtattacggc
      541 gatctggcca ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt
      601 tatctgccgg agggcacgct ggtcctggga aatacggctg cagatgggat ccgttgttac
      661 ggtgccattc aggatgcgca ggcgttgtcc gaaggtgtgg tggcttcttc ccgttacccg
      721 aaacactggc tgaccgtggg cgatccggcc cgtgaattta ccatgacgca gtccgcaccg
      781 ctgatggtgc tgccggaccc ggatgagttt gtggtggtgc aggtgaaata atccgtgagc
      841 gggggcgaaa tgcccccgtg tcttttttca cagggggctg atatggcaac aaaagaagaa
      901 aatcagaaac gtcttcgtca actggctggc ctgctggggc gcgaggcgga tatgtcgggg
      961 agtgctgcgg atattgcgca acgtgtgtct gagtgggaag aggagcttgc tgcttcccgg
     1021 gagggcatta tgcctggtga tgagagcggg cctgagcaaa atcacacaga cgatggtgag
     1081 cagttgcaca acactgatgc tacggatgat gttaaagcgg tccgtgtgcg gaaatgcctg
     1141 catgtgatgg ggtattgccc ggagacaggc cgtcccgttg aactgacgta ccggggcatg
     1201 cgtgttctgg tgccatcacc actggcgaca gccatgatac agcacggaac ggctgagcat
     1261 gcgtgatttt cagaatgcct ttgatgctgc ccttgccggg gtggacagca cgattgttga
     1321 agtgatgggg ctctgtgcgc agttcacctc gggggcacag tgtggcagcg aagttcaggg
     1381 ggttt
//
LOCUS       DAZUGK010000428         1373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZUGK010000428 DAZUGK010000000
VERSION     DAZUGK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.431"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..706)
                     /locus_tag="ACHY5W_004942"
     CDS             complement(<1..706)
                     /locus_tag="ACHY5W_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449648.1"
                     /translation="MWNFLRRTRKNQKSGRDVKEVGWTSLFQAVVEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEEN"
     gene            complement(652..873)
                     /locus_tag="ACHY5W_004943"
     CDS             complement(652..873)
                     /locus_tag="ACHY5W_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449649.1"
                     /translation="MAKIRYLQGTHDGRAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(918..>1373)
                     /locus_tag="ACHY5W_004944"
     CDS             complement(918..>1373)
                     /locus_tag="ACHY5W_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4449650.1"
                     /translation="EAAFGQFVTANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDM
                     TLLGGTFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPGGDS
                     TTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          289 a          460 c          341 g          283 t
ORIGIN      
        1 cattttcttc cgtaatactg ccggggatct caatcacccc ggacggcctg ccgccatttc
       61 tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg cgtggccgcc agcccggcag
      121 catacaccgg cggcagcccc acaagcggat gaaaaaaaca gttaaaccgg tcgtggatca
      181 cttccctggc aggcaccgtc accgcctccg tgatcccgca gttccggtcc ggtgtaatgc
      241 gatagaacac ctcgccgtca tccgccacca gaggttcaac ccggctccag tccagaatac
      301 gcagttcttt gatctgcccc cgggagttac ggattttcag caccaccgta ttgccgtgac
      361 gcagtttggc gttcagccac agttcaaaaa actggatacg attctgctgt gcattgggac
      421 gacgacagag acgggcaata tccccctgcc gtttttcacg gcggatcccc tgtgtatccg
      481 tctgcatcag gcgcagccgc attttggcga tatcctggga tatcagcgaa atgcaagaaa
      541 acaccgcatg aaaggagagg acactttccg gatcggcttt cacaccctgc tgccaggcgc
      601 cggcaaaggg ctcaaccacc gcctgaaaca ggctggtcca gcccacctct tttacatcac
      661 gtcctgattt ctggtttttt cgggttcgtc gcaaaaagtt ccacattcgc tatgctccgc
      721 atcacgtttc tttttctgac ctgccggacg tcgcaccgtg atgtactccg cctttcccag
      781 gcgaaccagc acctccgcac acggctgtgc gacatcacgg atatccccgg cccggccatc
      841 atgcgtgccc tgcagatatc ggatctttgc cataacctgt tacgggaggc gcacgcctcc
      901 cgtcctcctt atcagactca gccgccggac gcactgccgt agttcactcc ggtgatcacc
      961 gccaccgccg cggtacggcg acgacgccag ttgatccagc gctccgcacg gatggccaca
     1021 ctgcctgtct ggaacatgga aaccagctcc accggcgacg gtgtggtgct gtcgccgccc
     1081 ggctcagact gcatttccag tgacgcttca cgggacatat ccactgccac accgccgtca
     1141 tccgccagat aaatatccgg tgcattcacc agtaccagct ggtcacccac gtactgggag
     1201 acaatcaccg gaagcccctg aaaggtcccg cccagcaggg tcatgtccgg atattccttc
     1261 tgacccagcg catttttacg catggacagc gccagggcat tcgtgctgga catcagccag
     1321 acagcaccag tgggctgcag atttgccgtc acaaactggc caaacgcggc ctc
//
LOCUS       DAZUGK010000429         1373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZUGK010000429 DAZUGK010000000
VERSION     DAZUGK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.432"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            208..>1373
                     /gene="bluF"
                     /locus_tag="ACHY5W_004945"
     CDS             208..>1373
                     /gene="bluF"
                     /locus_tag="ACHY5W_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFS4449651.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALVFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKA"
BASE COUNT          395 a          289 c          289 g          400 t
ORIGIN      
        1 taatgcatat aaaacaatac gttatgataa aacatattgc atcatttaac tgacaaccta
       61 tacaaaaatt ttcgccaact cgtgttcaac tttttccatt ttttatcaat gtaaagaaac
      121 agttatgttt tggtattaaa agaaagacac cggaagatat gtctgttacc gtcttactct
      181 cgcctctccc attaccctgg acagttaatg cttaccaccc ttatttatcg tagccatata
      241 cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac
      301 atgcagtctg acgtaacagg gatcttactg tttaatggtt ctcatttttt ccagcttctg
      361 gaaggtccgg aagaacaggt taaaatgata tatcgggcta tatgccagga tccacggcac
      421 tataatattg ttgagctgct gtgcgattac gcgcctgctc gccgttttgg caaagcggga
      481 atggaattat ttgatttacg cctgcacgag cgagatgacg ttttacaggc cgtattcgac
      541 aaaggcacat caaaatttca gctaacttat gatgacagag cgctacaatt ttttcgtact
      601 tttgtccttg caaccgaaca atcaacctat ttcgagattc ctgctgaaga ctcctggctt
      661 tttatcgctg acggatctga taaagaactt gattcctgta ccctttcacc aaatataaac
      721 gaccactttg cctttcatcc tattgttgat cccttatcgc ggcggataat cgcttttgaa
      781 gccattgtgc aaaaaaatga agacagcccg tcagctatag cagttgggca gcgtaaagac
      841 ggggaaatct acacagcgga tctcaaaagt aaggcgcttg tattcgcgat ggcacacgca
      901 cttgagctcg gtgataaaat gatatctatc aatctattac ctatgaccct ggttaacgag
      961 cctgacgcag tctctttttt acttgatgaa ataaaggcca atggtctggt gcctgaacaa
     1021 atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg
     1081 gttaaatcgc taaaggctgc cggtatcagt gtagcaattg atcattttgg cgcaggtttt
     1141 gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg
     1201 attaccaatg ttcataaaag cggaccacgg caggcaatta ttcaggcgat cataaaatgc
     1261 tgtacatcac ttgaaattca agtctctgct atgggcgtgg caacaccaga agagtggatg
     1321 tggcttgaat ccgcaggaat tgagatgttt cagggggatc tgtttgctaa agc
//
LOCUS       DAZUGK010000430         1353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZUGK010000430 DAZUGK010000000
VERSION     DAZUGK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.433"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..243)
                     /locus_tag="ACHY5W_004946"
     CDS             complement(<1..243)
                     /locus_tag="ACHY5W_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449652.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNG"
     gene            complement(254..973)
                     /locus_tag="ACHY5W_004947"
     CDS             complement(254..973)
                     /locus_tag="ACHY5W_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFS4449653.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          345 a          360 c          264 g          384 t
ORIGIN      
        1 gccgttttgc gtcacttttt ctgagagctg gtacagataa gcgtcgagat tactcagaac
       61 atgatcacgt atctgggcgg cccgatcgcg ccactcctcc cagtgcccca attcatcgac
      121 cattttttgc cgattagccc caatacgctg ctgcgcgttt gccaccgctt tgcgcatgat
      181 cggatcttca atttgctgac ggatgcgtgt cttaaaatct gtattactgg ttttgatcga
      241 catctttata tcctcagcgg ctcatcaaca cttcagcaat atgcatcact ttgactttct
      301 gcccttcccg ttgtaatcgc ccactgatgt tcagcaggca actcacgtca gcaccaatta
      361 aatactcagg gcggacttcc atcaggtgcg caaccttttc tttcaccatc tcgccggata
      421 tttcggccat tttgaccgag aacgtgccgc caaatccgca gcaggtatcc tgttcagcaa
      481 aggtcaacag ctccagtcca cgcacatttt tcagcagcgt aagtggctcg tccttcaccc
      541 ccagcttacg ggccaggcta caagatgggt gatacaccgc tctcccttgc aaactggcac
      601 ctacatcgac tacccctaat ttattaacaa taaaagaggt gagatcctgc atacgcgcgg
      661 caaccttttc ggcacgtgat gcccattcag gttcatccgc cagatacgtc gggtaacttt
      721 ttacggcata ggtgcaagag ccagctggtg aaataatggg atcgtcgtta tcctccagtg
      781 cggcgatcag atttttcatc cctggaatcg cttctttgat ataaccacta ttgatcgcag
      841 gctgaccgca gcatccctgt ttctccggga aatttacgcg acagccgagt ttttccagta
      901 gcagcacgga gtctcgtgcc attcttgatt tcagggcgtc accaatacag gtgacaaaga
      961 aattgacatt cacaactaat actccattac ttcatgccca tttatgcccg atgtttcccc
     1021 cccaccgggg gagcaatcat caataaatat ggcgttcttt tttaattggc tagcaacata
     1081 ccacccctca tttgtatgac aaatgataat ctttcatcca aacttcaaat gcaataacta
     1141 attttatttc tttcggcatc gctcacattt tatggggaaa tcgacgatca tttttggcat
     1201 aagaaatcca tttaaaaatg gctacatccc ttatacctat tcagagctac ttattcaggg
     1261 gttattgaga tatacactga tggtgtacaa aaaaatcacc gacaataatc acgtatcaag
     1321 taatagtcgg gattttattt aattgaagca att
//
LOCUS       DAZUGK010000431         1350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZUGK010000431 DAZUGK010000000
VERSION     DAZUGK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.434"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            192..434
                     /locus_tag="ACHY5W_004948"
     CDS             192..434
                     /locus_tag="ACHY5W_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4449654.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            431..853
                     /locus_tag="ACHY5W_004949"
     CDS             431..853
                     /locus_tag="ACHY5W_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS4449655.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAIIPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSEDSVIVH"
     gene            866..>1350
                     /locus_tag="ACHY5W_004950"
     CDS             866..>1350
                     /locus_tag="ACHY5W_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFS4449656.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQKKQERNLTGVQTDVEVVFEHDVNTKAT
                     N"
BASE COUNT          403 a          286 c          332 g          329 t
ORIGIN      
        1 tgagataaac caagcttctg cctgcgctct ttaagacgca ttgcaatttt gtcattgagc
       61 ctactagcgg caattttttc gttttctttt tccattgcat ccttgtatca cgaatcgtga
      121 ttcacataaa acacaaaaca gcttgaccat ataacacaag gtgtgtttaa aattgtcatc
      181 ggaggttttc aatgaacaaa atttcaacat atcgaaaaca gcttgggctg tctcaaagac
      241 aacttgctgt tcagttaggg tggatacaaa gccgactggc aaattacgaa gcaaattttc
      301 gtacccctgg actagaggag tgcagaaaaa ttgtttctac ccttaatcgg cttggcgctc
      361 attgtggact tgacgatgta ttccccccag acggtaagca tagcgaaaac agcataggag
      421 cggttgattc atgaaaatca ggcatgagca catcgaatca gtgttgttag ccctggcagc
      481 cgaaaaaggg caggcgtggg tcgctaacgc aattactgaa gaatatctgc gccagggggg
      541 cggcgaattg cccctggtac caggcaagga ctggaacaat cagcagaata tctatcaccg
      601 ttggttgaaa ggtgaaacga aagcgcaaag ggaaaaaatt cagaaactga tccctgcggt
      661 tctggcaatt attccgcgcg agctgcgtca ccgactctgc atcttcgata ccctggaacg
      721 ccgtgcatta ctggcggcgc aggaagcgtt gagtacggca attgatgcgc atgatgatgc
      781 agtccaggcc gtttaccgga aagcgcattt cagcggcggc gggtcttccg aagattctgt
      841 cattgttcat taagcaaaag tttccatgct gtttgtgctt attctaagcc accgggcagc
      901 atcatacggg gcaattatgg ccgcattacc atacatgcaa ctgtacatag ctgattactt
      961 ggctgacacc atgcatttgt cagcagagga gcatggtgcg tatttgttgc tgatgttcaa
     1021 ttactggcaa acaggaaagc caatacctaa aaacagactg gcaaaaattg cccgtctgac
     1081 taacgagcga tgggctgatg ttgaaccatc cttgcaggag tttttttgcg ataacggcga
     1141 ggaatgggtg catcttcgga ttgaggaaga tctggcatca gtcagggaaa aattaaccaa
     1201 aaaatcagcc gcaggaaaag catctgttca ggccagaaga agcagaaagg aagcagatgt
     1261 tcaaaaaaaa caagagagaa atttaacagg tgttcaaaca gatgttgaag tggtgtttga
     1321 acatgatgtc aacacaaagg caactaataa
//
LOCUS       DAZUGK010000432         1348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZUGK010000432 DAZUGK010000000
VERSION     DAZUGK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.435"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            316..623
                     /locus_tag="ACHY5W_004951"
                     /pseudo
     CDS             316..623
                     /locus_tag="ACHY5W_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(872..1327)
                     /gene="espF"
                     /locus_tag="ACHY5W_004952"
                     /pseudo
     CDS             complement(872..1327)
                     /gene="espF"
                     /locus_tag="ACHY5W_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          387 a          321 c          286 g          354 t
ORIGIN      
        1 ccgccaaact tcctcttctg aactctcccg acgtcaacaa aacccctttt aatcccttat
       61 atatcaggct ttctttgtgt acaggggtca accgactcaa cctgtatcaa gttgcttttg
      121 aggatacttt aggggggtat tatttccctt tgaaatatta gcaccccata atgggaaaaa
      181 ggtaatacat aacaatggtt tttaacagta tctttgttat acaagggggc attgaggata
      241 tcaggaaaaa tctcaaaatc ggtacggaca ggaaactacg tgggcagcaa aaacgtatgg
      301 taacaccggg aaatgcaacg cccaccagtc gggctaatcc accactccaa tcgtggttcg
      361 cagtactgca cacacgatta ccgggctata caggagcggt ttggtctgaa aacatccatg
      421 ccgcgtaaag gtaaccgtta cgacaacacc tcaatgaaaa acttatagta aacgctgaag
      481 aatgaaacta tcgttttaat aaccgggatg aagccatcta agtaatacgg aaatacattg
      541 agattttcta caaccatcag cgccgccact cccgtctggg gaatatctcc ctggtagcct
      601 tcaggaaaaa tataaccaga taactgcgta aaaaaagaac aaatggtggt gtccgctatt
      661 gccagtatac ctgagtttac tgcctttcag attatcacaa tcaatagcga acagactttt
      721 gagctctgtg cgcttttaac aaaacgtccc tttctccact ttcaccgtat aataatacat
      781 tatttattac ccctccaata aaaaccgata ttactaatca catttattac cacacaattt
      841 gatatcggtt tttattagtt ggttaccctt tcttcgattg ctcataggca gctaaatgat
      901 cttttaatgc ctgtgcaagg ggcggtagtc cactaggccc tgtcggtggc ggtggtgcct
      961 gacgggcggg cttaaaacct aaagcctcag gacctttcga tttttcatag gcagccaagt
     1021 gctcttttaa tgcctgtgca atgggcggta aaggtcggga tgccccggat gcctgtccac
     1081 ttgtcggtgg cggcggtgcc ggacgagagg gagtaaatga agtcacctgg ctgctcacat
     1141 taaaaatcgt tctcgcatta acattcgacg agcctggaga aaaaggggaa tgaactttca
     1201 ccggagtaag acgcacggcc tgaggggcta cagaaaatcc agttcccccc gcagagctca
     1261 ctcgacttgc gatacctaca agctgccgcc ctagtgtaga agcagcgtta ctaattccat
     1321 taagcataat ttatgcctct ttatatct
//
LOCUS       DAZUGK010000433         1346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZUGK010000433 DAZUGK010000000
VERSION     DAZUGK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.436"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(3..863)
                     /locus_tag="ACHY5W_004953"
     CDS             complement(3..863)
                     /locus_tag="ACHY5W_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4449657.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
     gene            991..>1346
                     /locus_tag="ACHY5W_004954"
     CDS             991..>1346
                     /locus_tag="ACHY5W_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449658.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
BASE COUNT          363 a          361 c          259 g          363 t
ORIGIN      
        1 tattaaatat attttttaaa ccattgtgtt tgcacagctg ctacccgctc aaaaaatgtg
       61 ccctcataaa tatcataatg gcatgcgtca gcttcctcat ataattcttt agtaccagaa
      121 gcaacagcat catataatgc tcttccctgt tccggtggat taacactatc ctggccagca
      181 ataacaacca atactggaca ctggactctg gctgcgctct cggccggttt atattgcaac
      241 gtttccatga cagtaaggaa aggtattttt atatccattt cgggatgctg agctttgact
      301 ttctcgaaga ataccttcga ctcatcatca tttagtaccc gtgtaacccc cacaaacatc
      361 tccttccctg tgtttttctt tttctctgcc attttattca gtgtggacag aaaagaagct
      421 ctctccaatt cattcatctc tccggtaatc agagcatcac catcagcaaa agccagctgg
      481 ctgacaatac atttcactct ttgatcttgc gcagctgcac tgaacacatg acaaccaccc
      541 agcgatgttc cccataaccc gatacgctga ttatcaatac atgcctgctt ttctgcccaa
      601 ttgataactg aaataatatc ttcgatctgc attgctggaa ccagacggcc ccgttctcct
      661 tcactctcac cgaatccacg ataatcaaaa gtaatcgttg cgaaccctgc ttctgtgaaa
      721 gcattcgcaa agcaagggag cagaacattc cggataccgc aaaatccatg gcataagata
      781 atcaaaggat gtttcatatt tccttcagga acacgaaatg tcagagcaat accttcagaa
      841 agtttgtgat ccgtaatttt cataaatatc ctgtgttatg ttgtttatta atcatcagaa
      901 tcagcgccag catcatacca gtcacaatac cagaatgccc agcgcaatca gtatccataa
      961 acagtgcgtg tgctacgtga aaaataactc atgaacaatg tcattcccct gcagaattca
     1021 ccagaacgcg tctccctgtt acccattgcg ccgggggtgg attttgcaac agcgctctcc
     1081 ctgagaagaa tggccacttc cacaggggcc acaccggcct acctgctggc cccggaagtg
     1141 agtgcccttc ttttctatat gccggatcag cgtcaccata tgctgttcgc caccctctgg
     1201 aataccggaa tgcgtattgg cgaagcccgg atgctgacac cggaatcatt tgatctggat
     1261 ggtgtaagac cgtttgtgcg gatcctgtcc gaaaaagtgc gcgcgcgacg cggacgcccg
     1321 ccaaaagatg aagtccgtct ggttcc
//
LOCUS       DAZUGK010000434         1341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZUGK010000434 DAZUGK010000000
VERSION     DAZUGK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.437"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1273)
                     /locus_tag="ACHY5W_004955"
     CDS             complement(<1..1273)
                     /locus_tag="ACHY5W_004955"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4449659.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIM"
BASE COUNT          315 a          360 c          358 g          308 t
ORIGIN      
        1 acataatgcg catcaccacg atgtcggtgg cttcaccgcc gagagtgaag gccggaacct
       61 gccagccgcg cagacgcaga cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac
      121 cctctttcag tttgaagcaa accgccggga tgccttcgtc cgggcgaccc gtacagatga
      181 actcatacgg ccccagtttg gcgatttcat ccgccagata agcggcaacc tggtaagagg
      241 cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag tactgtgcaa
      301 ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga ccacccaggt
      361 agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag ataacccagc
      421 cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat ttcacacgcg
      481 gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag ccaccgctgg
      541 cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta tccagtgcat
      601 cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa gtcggcacca
      661 cgccgatggt gttttcgtcg caggcttcaa tcatgcgttt cgggtccata aacaactgac
      721 cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat ttatgccagc
      781 agatttgtac cggaccgcac accaggtttg gtttatccgt tggtttgcct gcagcttcca
      841 tacgtttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc tcggaagaac
      901 caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc cacagatcgg
      961 caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct tctttgtcga
     1021 tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg tcccaggtct
     1081 ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca tcattgataa
     1141 tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac tccgcgatag
     1201 tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa tccgttaaca
     1261 gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa gtaggattta
     1321 tccgcaatgg aagcaaggca t
//
LOCUS       DAZUGK010000435         1339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZUGK010000435 DAZUGK010000000
VERSION     DAZUGK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.438"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1339
                     /locus_tag="ACHY5W_004956"
     CDS             <1..>1339
                     /locus_tag="ACHY5W_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4449660.1"
                     /translation="LSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEA
                     IVDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLR
                     LTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAP
                     AVPATIELTPGYFQITAVPKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQW
                     SVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAAGYLELFREKIGKTHLAEA
                     LWAEIDNSQLKDEMAEMQTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLA
                     ALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTP"
BASE COUNT          326 a          362 c          409 g          242 t
ORIGIN      
        1 tcctgtccat cgatgccgcc agccgcaccc tgacgctgga ccgtgaggtg acactgccgg
       61 agaccggtgc cgccacggtg aacctgatta acggcagcgg taagccggtg agtgtggaca
      121 tcaccgcaca ccccgcgccg gaccggatac aggtcagcac cctgcctgat ggtgtggaga
      181 catacggtgt atggggacta tccctgccgt cactgcgtcg tcgcctgttc cgctgtgttt
      241 ccatccggga aaacacggac ggtacctttg ccatcacggc agtgcagcat gtgccggaga
      301 aagaagccat cgtggataac ggggcgcact ttgacggcga ccagagcggc accctgaaca
      361 gcgtcatccc tccggcagtg cagcacctga cggtggaggt gagcgcagct gacggccagt
      421 atctggcaca ggcgaaatgg gacacgccgc gggtggtgaa gggtgtgcgc ttcagtctgc
      481 gcctgaccag cggaagcgga gaagacagcc gtctggtgac caccgccatc actgcggata
      541 cagagcatcg tttcagtggt ctgccgctcg gggaatacac cctgacagtc agggcaatta
      601 acagttatgg ccagcagggg gaaccggcca ccaccacgtt caggattaat gcacctgcgg
      661 tacccgccac gattgagctg acaccgggct attttcagat aacggcagtc ccgaaactga
      721 ccatttatga cccgacggtg cagtttgagt tctggttttc ggaggcaaaa attgcagacg
      781 ccgcacaggt ggaaacctct gcccgttatc tggggaccgg cagtcagtgg agtgtctccg
      841 gtccgcacat taagcccgga aaggatttct ggttttatgt gcgcagcgtc aacctggtgg
      901 gtaaatctgc ttttgtggaa gccagtggac gggcgagcaa tgatgctgcg ggctatctgg
      961 aacttttccg ggaaaagata ggaaaaacgc atctggcaga ggcgctgtgg gcagagattg
     1021 acaacagtca gctgaaggac gagatggcgg aaatgcagac caccatcaca gaaacccgca
     1081 atgaaatcac acagacggtc agtaaaacgc tggaggacca gagcgccacc atacagcaga
     1141 tacagcgcgt gcagaaggac acaaatgatg accttgctgc actttacatg ctgaaggtac
     1201 agaaaacaaa aaatggcata ccctatgtgg ccgggattgg tgcagggatt gaggatactg
     1261 atggccagcc actgagcaac atactgctgc tggctgaccg tatcgcgatg ataaatccgg
     1321 agagcggcaa cagcacgcc
//
LOCUS       DAZUGK010000436         1313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZUGK010000436 DAZUGK010000000
VERSION     DAZUGK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.439"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..447
                     /locus_tag="ACHY5W_004957"
     CDS             <1..447
                     /locus_tag="ACHY5W_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4449661.1"
                     /translation="IHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRED
                     EWWRNGQNLYLDNMEATGFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHL
                     PEQLSKRERYSEKWQRRTHSVWRHRHWHVSAFTGIYNDLAAASACM"
     gene            444..1022
                     /locus_tag="ACHY5W_004958"
     CDS             444..1022
                     /locus_tag="ACHY5W_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS4449662.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTLIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          299 a          324 c          416 g          274 t
ORIGIN      
        1 attcacaaat tccgctgcgt tccgcacctg accggacggc gctttgaaca cggggtgacg
       61 gactgttaca ccctgttccg ggatgcatac catctggcgg ggattgagat gccggatttt
      121 caccgtgagg atgagtggtg gcgcaacggt cagaacctgt acctggacaa tatggaggca
      181 acgggctttt accgggtgcc cctgtcctct gcacaggcgg gcgatatcct gctgtgctgc
      241 tttggcgcat cggtggccaa tcatgccgcc atttactgcg gcaacggtga gctgcttcac
      301 catctgcctg aacaactgag taaacgggag aggtattccg aaaaatggca acgacgaacg
      361 cattctgtct ggcgtcaccg ccactggcac gtatctgcct tcacggggat ttacaacgat
      421 ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccatccgt gccctgtcga
      481 tgcagatgcc gggattccgc cgtcagatga acgaaggctg gtaccagata cgtattgccg
      541 gtgatgacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg ggtgagggaa
      601 cgctcatcca cattgtgccg cgactggccg gggccggaaa gggcggactg cagattgtgc
      661 tgggggcggc agccatcgtg gggtcgttct tcacggccgg ggcatcaatg gcgttatggg
      721 gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttct ctgggggcca
      781 gcatgattct gggtggtgtg gctcagatgc tggccccgaa ggctaaagta ccggagtaca
      841 aaagcacgga taacggcaga cagaacacgt acttttcctc gctggataac atgattgccc
      901 aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggttcacga cggatatccc
      961 aggacatcag cacccgtgat gagggcggag acgggaaagt ggtggttatc gggcggggat
     1021 gaaaataaaa aaatcccgca gagttagcgg agctgcggga gagaacgatg aagattaacg
     1081 ttatggagtt atttttcagg catcaaaaaa gtaatgcagc gtcattattg cggctacagg
     1141 caattgccgg aaatgtgaag agtttcagaa attttattcc gtcatgacac aggcaccctc
     1201 cggggtgcct gttgttttct ggcataaaca gattcagaca tcagacagga gagggggaca
     1261 gagtgggtaa agggggcggc aaggcgcaca cgccggtaga ggcaaaggac aat
//
LOCUS       DAZUGK010000437         1312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZUGK010000437 DAZUGK010000000
VERSION     DAZUGK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.440"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..643
                     /gene="evgA"
                     /locus_tag="ACHY5W_004959"
     CDS             29..643
                     /gene="evgA"
                     /locus_tag="ACHY5W_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFS4449663.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            648..>1312
                     /locus_tag="ACHY5W_004960"
     CDS             648..>1312
                     /locus_tag="ACHY5W_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4449664.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISR"
BASE COUNT          412 a          275 c          255 g          370 t
ORIGIN      
        1 agatgcttca ttgcaaaggg aataatctat gaacgcaata attattgatg accatcctct
       61 tgctatcgca gcaattcgta atttattgat caaaaacgat attgaaatct tagcagagtt
      121 gactgaaggc ggaagtgccg ttcagcgggt ggaaacactt aagcctgata tcgtcatcat
      181 tgatgtcgat atccccggag ttaacggtat ccaggtgtta gaaacgctga ggaagcgcca
      241 atatagcgga attattatta tcgtctccgc taaaaatgac catttttacg ggaaacattg
      301 tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca atatcattgc
      361 ggctattgaa gctgcaaaaa atggctactg ctatttcccc ttctctctca accggtttgt
      421 tggaagttta acgtccgacc agcaaaaact cgactcctta tcgaaacaag aaattagtgt
      481 catgcggtat attcttgatg gcaaggataa taatgacatt gctgaaaaaa tgttcatcag
      541 caacaaaact gtcagcactt ataaaagtcg cctgatggaa aaattagaat gtaaatcact
      601 gatggatctt tacacattcg cacaacgtaa caaaatcggc taaccacatg aagtttttac
      661 cctatatttt tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca gacgaagatt
      721 acatcgaata tcgtggcatc agtagtaaca accgtgtcac acttgatcca ctacgtctga
      781 gcaacaagga attacgttgg ttagcgagca aaaaaaatct tgtgattgca gtacacaagt
      841 ctcaaacggc tacgttgttg cataccgatt cgcagcaacg ggttcgtggt attaatgctg
      901 attatttaaa tcttttaaaa agagcgttaa atatcaaatt aacactccgg gaatacgccg
      961 atcatcaaaa agcaatggac gcgctggaag aaggtgaagt cgatatagtg ttatcacatt
     1021 tagttgcttc gccgcctctt aatgatgaca ttgctgcaac caaaccactg ataattacct
     1081 ttccggcgct ggtcaccacc cttcacgatt caatgcgacc gcttacctca tcaaaaccag
     1141 taaatattgc tcgagtagca aattatcccc ctgacgaggt aattcatcaa tcatttccaa
     1201 aagcaacaat tatctctttt acaaatttat atcaggcatt agcatccgtc tcagccggac
     1261 agaatgatta ctttattggt agtaacatca ttaccagcag tatgatttcc cg
//
LOCUS       DAZUGK010000438         1311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZUGK010000438 DAZUGK010000000
VERSION     DAZUGK010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.441"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..210
                     /locus_tag="ACHY5W_004961"
     CDS             <1..210
                     /locus_tag="ACHY5W_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449665.1"
                     /translation="MGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            207..>1311
                     /locus_tag="ACHY5W_004962"
     CDS             207..>1311
                     /locus_tag="ACHY5W_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4449666.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYV"
BASE COUNT          283 a          333 c          422 g          273 t
ORIGIN      
        1 atggggcgtc ttctggtgga gaacattaat accgacccca ggctggcgct ggatgcggct
       61 tataagctgg cgcagttcac gcaccataaa aaaggggatg ccggtaaaaa atcggcaaaa
      121 ggtgacgcgg cgaaaaaagc ggctaaccgt tttgcggtgc caccaccacc ccgcctggtg
      181 gtgaataatg ataatgaggg caacggatga tacctgtgtg gagcacggcc tgcccggact
      241 gggcagagcg cctgaaaaag gggctgtcga ttattccggc tccgatttat ccggaccagg
      301 ctgcacatgc actggcgatt tttaaacaac tgcgaattgt ggatgcaccg ggtagcccga
      361 cattcgggga gtcctgtgca ccgtgggtgt ttgacctggt ggcggccctg tttggctcct
      421 acgatgcgca gaccggtgtt cgccatatca aggaagtgtt tatccttatc cccaagaaaa
      481 acagtaagtc cacgctggct gcggggatca tgatgacggc gctgttactg aactggcggc
      541 aggcggcggg ctacaccatt ctggccccga ccgtggaggt ggcggctaac gccttcaacc
      601 ctgccaggga tatggtacga cgggacgatg atctggatga cctctgtcag gtgcagacac
      661 atatccggac catcacccac cgggtgacgg acaccaccct gaaggtggtg gcagccgatc
      721 cgaatacggt gtccggtatc aagtccgtgg ggacactgat tgatgaactg tggctgtttg
      781 gcaagcagta caaggcggaa gacatgctac gtgaagccat cggcgggctt gcctcccgtc
      841 cggaagggtt tgtggtgtat acgaccaccc agtcgaatga accgcccgcc ggggtgttca
      901 gacagaaact gcagtatgcc cgggatgtcc gtgacggcaa aattcatgat ccgcactttc
      961 tgccggtgat ttttgagcat cctcctgaaa tggtggaaag cggggctcac ctgctgatgg
     1021 aaaacctcgc catggttaac ccgaatctcg gttattcggt ggatgaggcc tttctgtacc
     1081 gggagtaccg taaagcccgg gaggctggtg aggaagcatt tcgtggcttc atgtcaaaac
     1141 acgccaatgt ggaaatcggt cttgccctgc gttctgaccg ctgggcgggc gcggatttct
     1201 gggagcagca gggcaggcgc gtcagcctgg acgatatcct gcagcgcgct gatgtggtga
     1261 cggtggggat tgacggcggg ggcctggatg atctgctggg aatgtacgtg a
//
LOCUS       DAZUGK010000439         1306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZUGK010000439 DAZUGK010000000
VERSION     DAZUGK010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.442"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1306
                     /gene="espL4"
                     /locus_tag="ACHY5W_004963"
     CDS             <1..>1306
                     /gene="espL4"
                     /locus_tag="ACHY5W_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFS4449667.1"
                     /translation="QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSR
                     PEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHY
                     VVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPAL
                     PKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAY
                     NSINLPGLYLAINYGNADIVETIFNSLSETGYEGLLSKKNLMHILEAKDKNGFSGLFL
                     AISRKDTNVVTSILNALPKLAATNHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADML
                     KIVLNALPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSL
                     AQEINISASDIVDLLTTKSLARDTGLFMAMQRGHMNVINTMFN"
BASE COUNT          422 a          243 c          257 g          384 t
ORIGIN      
        1 caatatatta atgatattta tcagaatgtt gattacaaac cccatcaaga tgattactct
       61 tcagctgaaa aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg
      121 atttccagcc gtcctgaggg gcgttgtgtt gctgcctgcg gtgatttcgg gctagttatg
      181 aaggcatatt ttgacaagat ggaatcaaat ggcatcagtg ttatggctgc catattattg
      241 gtagataacc atgctttgac ggcccgacta agaataaaga acacaactga aggatgtacc
      301 cattacgtgg tttcggttta tgatccaaat gtaactaacg ataaaataag aatcatgagc
      361 gaaagtaaag aggatattaa acactattct ctgatggatt ttatgaatgt tgattatagc
      421 ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt
      481 ccgaaagaac agctattgat gctaaaagga tctgtggatg aaataacccc tccattatca
      541 ccagcaacga tgaatttgct aatggcaatt ggtcagaatc accaacttac gcaactgatg
      601 attcaactcc agaaaatgcc agaactacat agaacagaaa tgttgactgc ctataatagt
      661 attaacttgc caggtttata tttggctata aattatggta atgcggatat cgttgagact
      721 attttcaatt cattgtcaga aacaggatat gaagggttac tctcgaaaaa aaatctcatg
      781 catattctgg aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt
      841 aaggatacaa atgttgtaac ctcgattctg aacgccttac ctaaactggc cgcaacaaat
      901 catttagata acgaacaagt gtataaattc ctgagtgcca aaaatagtac gtccagccat
      961 gttttatacc atgttatggc gaatggtgat gccgacatgc tgaaaattgt tttgaacgcg
     1021 ttacctttgt taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag
     1081 gcaaaggatt tttatggttg cccaggacta tacctggcga tgcaaaatgg acatagcgat
     1141 atcgtaaaag ttattcttga agcattgccc agcctggccc aggaaattaa catttcagct
     1201 tccgatattg tagatcttct gaccactaaa agtcttgcgc gcgacacggg tttgtttatg
     1261 gccatgcagc gcggacatat gaacgttatt aatactatgt ttaacg
//
LOCUS       DAZUGK010000440         1303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZUGK010000440 DAZUGK010000000
VERSION     DAZUGK010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.443"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..523
                     /locus_tag="ACHY5W_004964"
     CDS             <1..523
                     /locus_tag="ACHY5W_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4449668.1"
                     /translation="RKSNIINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKV
                     LRDQIGKHHKWVFVHTKAAKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDL
                     RHTWASWLIQSGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPN
                     MSHSGIMEDIKKA"
     gene            complement(539..615)
                     /locus_tag="ACHY5W_004965"
     tRNA            complement(539..615)
                     /locus_tag="ACHY5W_004965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(579..581),aa:Arg,seq:tct)
     gene            858..>1303
                     /gene="fimZ"
                     /locus_tag="ACHY5W_004966"
     CDS             858..>1303
                     /gene="fimZ"
                     /locus_tag="ACHY5W_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFS4449669.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTAKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITV"
BASE COUNT          384 a          283 c          303 g          333 t
ORIGIN      
        1 gagaaagtcg aacatcataa atctggaatg gcaacaaatc gacatgcagc gacgagttgc
       61 ctgggtgaat ccagaagaga gcaaatcaaa ccgcgccatt ggtgtggcgc tgaacgatac
      121 cgcctgtaaa gtgttgcgtg atcaaatagg caagcatcac aaatgggtgt ttgtacatac
      181 caaggcggct aagcgagcag atggaacatc aacgcctgcg gtcaggaaga tgcgcatcga
      241 cagcaagaca tcatggctat cagcttgtcg tcgtgcagga attgaagatt tccgtttcca
      301 tgacctcaga cacacctggg caagctggct gattcagtca ggcgtcccat tatcagtgct
      361 tcaggaaatg ggcggatggg agtccataga aatggttcgt aggtatgctc accttgcgcc
      421 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
      481 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
      541 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
      601 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
      661 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
      721 ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga
      781 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
      841 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
      901 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
      961 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     1021 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     1081 agagcacagc gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     1141 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     1201 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg ctcaactata
     1261 taaaaagcaa taaatgtagt acgaatagtt caacgatcac tgt
//
LOCUS       DAZUGK010000441         1299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZUGK010000441 DAZUGK010000000
VERSION     DAZUGK010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.444"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1299)
                     /locus_tag="ACHY5W_004967"
     CDS             complement(<1..>1299)
                     /locus_tag="ACHY5W_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4449670.1"
                     /translation="PEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQ
                     VLARWDTPKVVKGVSFLLRLTVTAGDGSERLVSTARTTETTYRFTQLALGNYRLTVRA
                     VNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKR
                     IADIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSTFVEAVGRASDD
                     AEGYLDFFKGLITESHLGKELLEKVDLTEDNASKLEQFSKEWKDANDKWNAMWGVKIE
                     QTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMN
                     DVFLKRLTAPTITSGGNPPSFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQI
                     KGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQ"
BASE COUNT          251 a          388 c          326 g          334 t
ORIGIN      
        1 ttctgatcat cactgattct taccgtgatg gtgccactgg cataactgtt cgtgcggggg
       61 aaagacttgc tgaccgtttt gacaatatcg ccttcaatct gattggctga cagtttcccc
      121 ttaatctgac agttctcatt aatcgtgacg ttgttgagcg tcccggagtt cgcattcaca
      181 ctgccactga tatccgcatt tttagcagtc agctttccgt ccggtgtcag ggaaaatgac
      241 ggtggatttc caccgctggt aatggtgggg gccgtcaggc gcttcaggaa cacgtcgttc
      301 atgaatatct ggttgccctg cgccacaaac atcggcgttt cattcccgtt tgccgggtca
      361 ataaacgcga tacggttagc ggcaaccaga aactggctca gtttgccttc ctccgtgtcc
      421 tccatgctga ggccaatacc cgcgacataa tgtttgccgt ctttggtctg ctcaattttg
      481 acgccccaca tggcattcca cttatcgtta gcatccttcc actctttcga aaactgctcc
      541 agtttgctgg cgttatcctc cgtcaggtcg actttttcca gcagctcttt accgagatgg
      601 gattcggtta tcaggccttt gaaaaaatcc aggtaacctt cggcatcatc gctcgcccga
      661 ccgacggcct ccacaaatgt cgatttgccg actgtgttca cactgcgaac gtaaaaataa
      721 taatcatggc ccggcttaat attgatactg gcggctatcc agtacagcgc cgtgccaaga
      781 tagcgggctg tggtttcaac ctgcctgata tccgcaatcc gtttttccga gaaccagaac
      841 tcaaactgta ccgtcgggtc ataaaccgca agatgtggcg tggcggttat ctgaaaatag
      901 cccggtgtca gctcaatccg agacggcgct gccggtgcgg caatccggaa cgataccgat
      961 gccggatcgc cctgctgtcc ccacgcattt accgcccgga ctgtcagtct gtagttcccc
     1021 agcgccagtt gcgtgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc
     1081 tcactgccgt cacccgctgt tacggtcaga cggagcagga agctcacgcc cttcaccacc
     1141 ttcggcgtgt cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggcg
     1201 gtcaggtgct gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca
     1261 aagtgcgccc cgttatccac gatggcttct ttttccggt
//
LOCUS       DAZUGK010000442         1296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZUGK010000442 DAZUGK010000000
VERSION     DAZUGK010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.445"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..458
                     /gene="terW"
                     /locus_tag="ACHY5W_004968"
     CDS             <1..458
                     /gene="terW"
                     /locus_tag="ACHY5W_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFS4449671.1"
                     /translation="TRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALKELRE
                     SYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDKDKKT
                     AVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            719..1048
                     /locus_tag="ACHY5W_004969"
     CDS             719..1048
                     /locus_tag="ACHY5W_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449672.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            1035..>1296
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004970"
     CDS             1035..>1296
                     /gene="tnpB"
                     /locus_tag="ACHY5W_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449673.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQ"
BASE COUNT          332 a          347 c          315 g          302 t
ORIGIN      
        1 ataccagaca ggcccggatt tttaagcttg ccaacttact gggcaccggc aagcccgttt
       61 ccgctgccga cattataacc agtctggaat gctctgagcc gacattaacc cgggccctca
      121 aagagctacg cgagtcatat tctgcggaga tcaaatacag caaggcaggt cattcctatc
      181 atctcgttaa tccgggccag ttggataaaa agaccctccg ccggatgaat gaagcgctcg
      241 cacagaatgc tgaactgaaa accggtgagt ccacagggaa ggtcgtactc gataaggata
      301 aaaaaacagc cgtgtcatta tcgctacgca tgcggattct aagaaaaatt gaccgtctgg
      361 ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa gctggctctg cactccgttg
      421 atgaactgat aaaagaatac agcgctaaaa agtcctgacg ctaaatctca gaccggggtg
      481 aaaactccgg ttttgtcata tttcacctct gtattggcgt gatgcaaaat ggcatctgaa
      541 tcgatcatgg tttggcatcc atttccgatc acagtttggc atcacaatcg atcacgattt
      601 ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta cggggaagtc atcttcacag
      661 ggatcttcag tcagttagca ttaccgacat ccattcacca acttaccgga gataatcaat
      721 gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac gttgccgcat ggcgcgccag
      781 cggccttacc cgtgaacagt actgtgaact ttacgacatt ccgtttaaat ccctgcgcca
      841 gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga gcaccagaaa ttattcctgt
      901 ttcagtatct ggctcttccg gaatgactga tggcagacca ttgtcggatg agcctgtcac
      961 tctcttcctg cccggtggta tccggatgtg ctgccagcct tctcagttaa ccgatgtttt
     1021 ccgggctctt cgccatgctg acgcctgata acgtattcct ggtcgtaaaa cccgtcgata
     1081 tgcgccgggg tatcgacaca ctgacacagt acgtccagaa cgaacttaac gctgcatggc
     1141 atgacggtgc tgcttttgtc ttcaccaata aagtccgctc ccgcataaaa gttctccggt
     1201 gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca ccggggaagc ttccgctggc
     1261 cccggaaagg tgatgccaca tggcatctca cacagg
//
LOCUS       DAZUGK010000443         1292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZUGK010000443 DAZUGK010000000
VERSION     DAZUGK010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.446"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..911
                     /gene="espB"
                     /locus_tag="ACHY5W_004971"
     CDS             3..911
                     /gene="espB"
                     /locus_tag="ACHY5W_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFS4449674.1"
                     /translation="MVNSASESTTGASSAVAASALSIDSSLLTDGKVDICKLMLEIQK
                     LLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVGGIISSALGIL
                     GSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVADVAEEASSAMQ
                     KAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVDKLTNTTAFVA
                     VTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDVRRTQDDITTR
                     LRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            918..>1292
                     /gene="cesD2"
                     /locus_tag="ACHY5W_004972"
     CDS             918..>1292
                     /gene="cesD2"
                     /locus_tag="ACHY5W_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFS4449675.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETED"
BASE COUNT          368 a          262 c          329 g          333 t
ORIGIN      
        1 cgatggttaa ttccgcttcg gagagtacga ccggcgcttc cagtgcagtt gccgcatctg
       61 ctttatcaat tgattcatct ctgcttactg atggtaaggt tgatatttgt aagctgatgc
      121 tggaaattca aaaactcctc ggcaagatgg tgactctatt gcaggattac caacaaaaac
      181 aattggcgca aagctatcag attcagcagg ccgtttttga gagccagaat aaagctattg
      241 aggaaaaaaa agccgcggca accgctgctt tggttggcgg gattatttca tcagcattgg
      301 ggatcttagg ttcttttgca gcaatgaaca acgcggctaa aggggctggt gagattgctg
      361 aaaaagcaag ctctgcatct tcaaaggctg ctggtgcggc ttctgaggtt gcaaataaag
      421 ctctggtcaa ggctacggaa agtgttgctg atgtcgcaga ggaggcatcc agtgcgatgc
      481 agaaagcgat ggccacaaca acgaaagcag ccagccgtgc atctggcgtt gcagatgatg
      541 ttgcgaaagc ctctgacttt gctgaagatc ttgcagacgc cgccgagaag acaagcagaa
      601 tcaataagtt gttgaattcc gtagataaac tgaccaatac cacagcattt gttgccgtga
      661 ccagtcttgc tgaaggtacg aaaacgttgc caacaacaat atctgagtcc gtcaaatcga
      721 ctcatgaggt taatgaacaa cgtgcgaagt cgctggaaaa cttccagcag gggaatctgg
      781 agctgtataa acaagacgtt cgcagaacgc aggatgatat cacgactcgt ctgcgtgata
      841 taacatccgc tgtccgcgat ctccttgagg tccagaatcg tatggggcaa tcgggtcgct
      901 tagctgggta attgatcatg gtcgatacgt ttaatgatga agtgtttaat tactatcttg
      961 aacaaaaagg gtacacaata cagaaggagt ttctttgtgg cagtgccttt tttatcggat
     1021 ggcggattga gacacctttt ttttcattag cgtacagact ggatgaacaa gaactgattt
     1081 tgtgctcttt tgaagcacgt aaccaaacag ggcttaacgg ccctgtttta tcgctgactc
     1141 atttgctcga agagttgtat caccattttt cgggtattaa gaaaatcagc gcgatgaaat
     1201 ctaagattgg ttcagattca gaacgtcaaa agcgcgaaga gttgtttaat tacttcatca
     1261 gaaagggtgc cgtgcagcaa gaaacagaag ac
//
LOCUS       DAZUGK010000444         1290 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZUGK010000444 DAZUGK010000000
VERSION     DAZUGK010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.447"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHY5W_004973"
     CDS             complement(<1..194)
                     /locus_tag="ACHY5W_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449676.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHY5W_004974"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHY5W_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(573..995)
                     /locus_tag="ACHY5W_004975"
     CDS             complement(573..995)
                     /locus_tag="ACHY5W_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS4449677.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQKTTKHD
                     VVRINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(1009..>1290)
                     /locus_tag="ACHY5W_004976"
     CDS             complement(1009..>1290)
                     /locus_tag="ACHY5W_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4449678.1"
                     /translation="ITPATAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSAD
                     PSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
BASE COUNT          282 a          387 c          299 g          322 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacat
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctcg ccttcgaatg cttttttgat
      601 gttaccggct cagtcgcttc tgttttcccg gttggttcag gatcggcatc gtgccggtta
      661 tccagcatac ctgaaagata aagcacccgg ttcgttgcct gattcagtgc atcagcaggc
      721 catcccagca tcacttcacg gcggatctgc tgcatctctg tctccggaga ggccagagtg
      781 cctttgaggg aatgggaatg ccatagcgac atcgccacaa gcagggatgc cgtttccaga
      841 tatcgctggt taatgcgcac gacatcatgc ttcgttgtct tctgttgttc tgcgtctgaa
      901 acaaacttca tataatcaaa ccgctgcagc gcagacaatt cggaaagcgt gacggacaca
      961 ccgttatatt caaattgttc tgttttcaga aacatgtatt acctccgttt accctgcagc
     1021 gcccgcttca gtaacggtga cttcagccac tgcggcgaac tgaccatttc cgctcaccac
     1081 agggatctgc accttacctg tcgccacgcc gtttaccgta attgtcatat ctttcacact
     1141 aatggtggct ttcgacggat cggcggaaac cgctctgaac gtcttgtcgg ttgcactttc
     1201 cggctcaaaa gaaaccgtca gggtggttgt tttccctttt gccaccgtac cggatgtcgg
     1261 tgtcacctta attgcagtgg ccggcgtaat
//
LOCUS       DAZUGK010000445         1284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZUGK010000445 DAZUGK010000000
VERSION     DAZUGK010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.448"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..75
                     /locus_tag="ACHY5W_004977"
     CDS             <1..75
                     /locus_tag="ACHY5W_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449679.1"
                     /translation="ERILDTPEWKDYRPKYSGSTYKYS"
     gene            complement(452..>1284)
                     /locus_tag="ACHY5W_004978"
     CDS             complement(452..>1284)
                     /locus_tag="ACHY5W_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFS4449680.1"
                     /translation="VDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVD
                     LTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREG
                     DIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIIL
                     CRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADR
                     FSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK
                     "
BASE COUNT          394 a          271 c          219 g          400 t
ORIGIN      
        1 gaaagaatat tagatacacc agagtggaaa gactaccgtc caaaatactc cggttccaca
       61 tataaatatt cttaatgata gcaaaaaata tattttcgat ataatcaatg ttatgattaa
      121 agagtatttc atcagggcag gtaaaaacag agtaaatcag caaaagaagc tgatcttcag
      181 cgatactgac actaactgac ggtttaagcg gtcgtatgaa gcagcagctt tccgacggac
      241 tgccatgcgg atcgtttacc ttttgggcta ttccgcccgt catcaagcgg ctcacgagta
      301 ctgagtttat caggatgata ttgctgacaa tggtaattcg ttgaccgatg tgtacttcac
      361 tatatatcgg tcaacaccgt actgccgtgc aacatctcca agatgtagca tcgtttttac
      421 cagaggaggc aggacataga ttgtaaaaaa actatttcga tgcatttacc attgagatat
      481 tttccttcat accaaaagtt ctatcaatta tcgcaagtgg ttcaacaggt gcggtcttat
      541 ctataaccca accactctct gtaacacttc ctcgtgtgat atattttgcc tttactttaa
      601 caactattag ataatgctca cgactaaacc gatcagcaac gctaagatcc gatgtgtatt
      661 caggcaatat tcttccaata cgtacctgct gttgtgccat atattcatct ggtgctttta
      721 catttggatt tgaatcagga agccctccag cagttttata ttcaacgata ttttcagctt
      781 gaataccggt tgtgccgcga cacaatatga tgtctgaatc ggacagttct atgtgcccct
      841 ccttaatttt cgaataaatt tgtttaatac tatgttctgg agttggtact atttcagaag
      901 ataaagcagg caacgtgtct tcaagcaccg gagaatcatc gtatatatcc aacaaatcct
      961 catcataccc aatatctcct tctcttaact gagaatacgg tttatctatg tatctaataa
     1021 ctcccccacc atgaagacct tctgatttat aaaatgtacc atcagcgtca cgaaagaaat
     1081 aaacattaac ctgtttgtta tttatagaat acctacctga atataattcc gaatttggct
     1141 ccttagttaa atctactgat tcccccttgt agactatttt atttggtgtc tctgaagata
     1201 tggggttaaa ggattctata tatctataaa aaccaccgcc aactttctca taatacccat
     1261 acttagaaga gttaagatct actg
//
LOCUS       DAZUGK010000446         1283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZUGK010000446 DAZUGK010000000
VERSION     DAZUGK010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.449"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(160..1260)
                     /gene="ompC"
                     /locus_tag="ACHY5W_004979"
     CDS             complement(160..1260)
                     /gene="ompC"
                     /locus_tag="ACHY5W_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS4449681.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          364 a          285 c          271 g          363 t
ORIGIN      
        1 caataaatat tatagtaaga tgtccccgga gggtatggaa taatattaat tgaggtgaat
       61 gatcagaaat atcaaaaatt gctcattttt acttaaggag tcatacagca gcacttaaat
      121 caaaaaccag cccagagaag ggctggtcag gagaggatgt tagaactgat aggtaatgcc
      181 cgttgcgacg atgttatcgg tgcgaatatc tacggcacgg gtaaagtcgc tttcatcaat
      241 caggttgatt ttataatcaa cataggtcga catatttttg ttgaagaaat acgtcgcacc
      301 aacgtcgata tactcaatca tgtcctggtc gccgtagcgg cccagatctc tgcctttcga
      361 tttcaggtaa gcgagtgatg ggcgcaagcc gaagtcgaat tgatattgcg ccaccacttc
      421 aaaactttgt gctttgttcg agatataacc atccccatag gtggtcatgt ttaatgtttc
      481 agcgtaatta gctgcaaagt agatgttgtt ggcatcatac tttatacctg tggcccacag
      541 ttctgcattc ttaccggagg cattgagagg attagcagcg gctctttcct gcgcatttgt
      601 ccgatcggat ttggtatata ccgcaccgat accaaagcca tcataaacat aagttgagga
      661 gataccgaaa ccgtcaccgt tggcacgcgt gtagtcagca ccataaagat gactgttgtc
      721 agtacgttca tttttgcctt gatattgcgc ggcaaagttc agcccttcaa ttaagccaaa
      781 gaaatcgtag ttgcgatagg ttgctacacc ggtggtacgg tatgtcatga agacatcttt
      841 ctgactccag gtatcgccac caaattctgg caacatatcg gtccatgaac caatgtcata
      901 gccgacaccg tagttacgac catagtccag agagccaaat tcactaattt ttaaaccagc
      961 ataggccaga cgggtcttgt tgccgcgcga gccgttggct tcatcgttat agcctttaaa
     1021 atcatattcc cagtgaccaa aaccaatcat ttgatcattg atttgcgttt ctcctttaaa
     1081 gccgagacgg gcataagttt tatcaccatc gtcacgctta tcatcagtaa aataacgtag
     1141 agcggtaacc ttgccataaa ggtcgagttt attaccatcc ttgttataga cttcggcagc
     1201 gtgtgctacg ttcgcagcta acaaaccagt tacaaccacc gcaactattt ttaatttcat
     1261 ttaacagaat ccttttaatt atc
//
LOCUS       DAZUGK010000447         1279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZUGK010000447 DAZUGK010000000
VERSION     DAZUGK010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.450"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..188)
                     /locus_tag="ACHY5W_004980"
     CDS             complement(<1..188)
                     /locus_tag="ACHY5W_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFS4449682.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSA"
     gene            complement(276..>1279)
                     /locus_tag="ACHY5W_004981"
     CDS             complement(276..>1279)
                     /locus_tag="ACHY5W_004981"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFS4449683.1"
                     /translation="GFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLAL
                     THSTSDFGNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQ
                     VREGAEYKYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATI
                     GDLVYKVLTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIR
                     PAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYL
                     AAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
BASE COUNT          271 a          398 c          316 g          294 t
ORIGIN      
        1 tcagcagact gtttgggcag caggaacacg ccttcggcaa aaccgtcacc ggcctcaccg
       61 gcaggaatat cggtaatggc cacggccacc atactgccga ccaccaccgc agcaccgctc
      121 aggatggtct gatctccggc attcaccagt tcaatggtgg taccgtcctg tacaaaattt
      181 ttcgccataa tgctgtttct ccggacagcc cctgtggggc tgtttttcag gcataaaaaa
      241 agccctttcg ggcagtgatt gtgataacgc ggttatcagg ccaccgacga acgcaccagc
      301 ccgcgccagt caagtggtgc cactccggca tcaatacgga tttttgtggc aatgccgtca
      361 gtggtgaaac cttcctgctg atcaatgtat ggcgtgtcca caccatccag ccaggccact
      421 tcaatggtgt cagtgccctg tgccgccgcc agataccagg ttttcgggtc tgccgcatca
      481 agacgcgctt ctgcaatcac ctcagcaaag ttctggatag ggttaatgac accggcgttt
      541 gcatccgccc ctttcacact ggccgatttg atggtctggt tcgccaccgt ctccagtgcc
      601 accggtacca gcataaaggc cggacggata ttcagggcac gatcgccttc tttctgcagg
      661 cgcatcatct gacgggccgc atccagtccg gaaacggaga tccccccggt ggcaatattt
      721 ttgtgatcgg catggaacag cgccttaccg tcggacagtt tcgggttatc cgtcagcacc
      781 ttgtagacca ggtcaccaat cgttgccttc gccgcacgcc ccatcttcat cggcacgtcc
      841 accagcatat tcagatcatc attgataatg gcctggcggg tgatggagaa aatctccccg
      901 taagtggcca gtgcaatggt ctccttgcga tctgaggtgg tgatgtattt atactccgcc
      961 ccctcacgaa cctggcgcag agaaccaaaa ccgcccatcc ccacgcgata cgctgttttg
     1021 aagtctgaca ggcgtccctt acgggtccac ttctggaagg tttcttctga ttcctcccag
     1081 ccctggatca gccccttgtt cgacacatcc agcagaatat tgccaaaatc agaggtgctg
     1141 tgcgtcagcg ccagcccgac catctgcatg gggttataac tggccacccc aataccgcgc
     1201 tccgtcagtg acatgcgagc ccattcacgc agggtcatcc cgttataggc gttatccttc
     1261 tcgacatttt caaatccgg
//
LOCUS       DAZUGK010000448         1277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZUGK010000448 DAZUGK010000000
VERSION     DAZUGK010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.451"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..405
                     /locus_tag="ACHY5W_004982"
     CDS             <1..405
                     /locus_tag="ACHY5W_004982"
                     /inference="COORDINATES: protein motif:HMM:NF019008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFS4449684.1"
                     /translation="HEGEIMQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNM
                     SVLQSSMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIML
                     AKQKVLSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            519..1277
                     /locus_tag="ACHY5W_004983"
                     /pseudo
     CDS             519..1277
                     /locus_tag="ACHY5W_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    799..909
                     /locus_tag="ACHY5W_004983"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          362 a          261 c          357 g          297 t
ORIGIN      
        1 catgaaggag aaattatgca gaaaagcaat aaagttataa tcagctgtgg tatggcatta
       61 tattttttgt gtggggggat tacagtggca gaagaacttg atgaaagcat gcaagttctt
      121 gcaaggaata tgagtgtact tcagtcatca atggataaaa aggagcttct tgaagctctt
      181 gacagaatgg atgatgctgt agatgagtca atgaagcgtc ttcctgagag catttcaccg
      241 gctgatgaag aagggaagaa tgactatata aaagaacttc aaaagcttga aaaagagatt
      301 atgctggcga aacaaaaagt cttatcggga cagttatcag atatacccga ttcggtaaac
      361 aagatgaata aaatcagagt tgaggggcat gataaattca gataaatcag ttggttgatt
      421 cctgcgggac gaatacatac gtcaggaaca taactttatg gcagtgaacc gccccgggaa
      481 tcctggagac taaacttcct gagaaagagg taaacaggat gactaaaaat actcgttttt
      541 cccccgaagt ccgtcaacgg gcgattcata tggttctgga aagtcagggc gaatatgact
      601 cacaatgggc ggcaatttgt tccattgccc aaagattggc tgtacaccgg agactctgcg
      661 tgtctgggga cgccagcatg agcgggatac cgggagcggt gatggtgggc tcaccagcgc
      721 tgaacgtcag cgtctgaaag agctggaacg tgaaaaccgt gaactgcgcc gcagtaacga
      781 tatccttcgc caggcttccg cttattttgc gaaggccgag ttcgaccgcc tctggaaaaa
      841 ataatgccac tgctgctgcg tgagcagtac ggggtcggac cggtatgcag tgaactgcat
      901 attgccccgt caacgtatta ccactgtcag caacagcggt atcatcctta taaacgtagt
      961 gcctgtgcac agcgcgatga ctggctgaag aaagagatac tgcgcttata cgatgagaat
     1021 catcaggtat acggtggcgt aaagtctgac gtcagttgtt acaggaaggt atcagggtgg
     1081 ccagatgtat agtggcgcgc ctcatggcgg ttatgggact tgccggtgtt ctccggggta
     1141 aaaaggtccg cactaccgtc agccggaaag ctgttgccgc aggcgacagc gtaaaccgcc
     1201 agttcgtggc agaacgtcct gaccagctgt gggtggcgga ttttacttac gtcagcacat
     1261 ggcagggctt cgtctgt
//
LOCUS       DAZUGK010000449         1237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZUGK010000449 DAZUGK010000000
VERSION     DAZUGK010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.452"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..>1237
                     /gene="espK"
                     /locus_tag="ACHY5W_004984"
     CDS             14..>1237
                     /gene="espK"
                     /locus_tag="ACHY5W_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFS4449685.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRN"
BASE COUNT          429 a          278 c          234 g          296 t
ORIGIN      
        1 atggaattta tcaatgcttc ctacatcgca attacgaccg accgggacat tctgctccta
       61 ttccgctgaa acatcagcag acatcaaaag cgaaatcaca ccaattcaga tagaagaagc
      121 gcgggccagt ggtcgtttat atatcaaaga ttgtgatatt gagtatctgc cacagttacc
      181 aaacgaaata acatcagtta caatcgaaaa ctgcaacaac ctgacaaccc ttacaggatt
      241 gccggttaat acacaaaacc tctccgtcat taactgtgaa aaattacaaa tcacagacat
      301 gccatcaacc gtaaaaaatc tacatattga attaactgat tcaccattta tacatttcat
      361 atctgaaggc atcgagtgcc tgacggtttg ccactgctat atatctggag tgccagagag
      421 tgtccgctac cttgaaataa aaggtagcgc cacagacagc ataaaaaatg ttccaaacgg
      481 gttatcatct ctcagcatca atagctataa cccggagaat caggccagaa ttgacaacct
      541 gatatcaccg tcactgaaga cgctatcgct gactggatgt agcaatatta tactgccgga
      601 gaaacttccg gagagtgtga catcggtaac cattcatgcg gagcagaaaa ccacgtggaa
      661 catcggtgtt gaagggatgc ctgatgggct ggatcttgat ttacaaaatg tactactctc
      721 tccagatgta gttaaagcaa aaaacatcac ctttcagggc aacgctctgg atgtggcctt
      781 acactttcgc gagggagaca ttgtctatgg actatcttca cccagagaaa aacttgtcaa
      841 cagcattaaa ctagttaacg acttttccaa aaaagatatt ataactcaga atacgttaac
      901 aaacgcagta tgggacccca gaacacctcg caaatataag caagatccac ttatcaaaag
      961 agcattaaat gaacacgaaa gagggataaa atttaaacaa cacttaaaga atcacaataa
     1021 ttataatgtt accatggccg acctttccgt atacaatcgc gacaaattat gggcaaaaac
     1081 aagcaaggcc ggcctagagt ttcagacatt aacacgcaat aaaacggtta ttttttgtgc
     1141 ggatgagctt gtcaactcac tcaaactcat agctaacaag tcagagggct atggccagag
     1201 tattaccgcc agcgaattac gatggattta ccgtaat
//
LOCUS       DAZUGK010000450         1231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZUGK010000450 DAZUGK010000000
VERSION     DAZUGK010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.453"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..182)
                     /locus_tag="ACHY5W_004985"
     CDS             complement(<1..182)
                     /locus_tag="ACHY5W_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449686.1"
                     /translation="MTEKRHGALNALVVANTGLSTCWLFRQELLVCHVNRKREKDMLK
                     QQDMTETARVVFNELSV"
     gene            complement(284..838)
                     /locus_tag="ACHY5W_004986"
     CDS             complement(284..838)
                     /locus_tag="ACHY5W_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS4449687.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(847..1206)
                     /locus_tag="ACHY5W_004987"
     CDS             complement(847..1206)
                     /locus_tag="ACHY5W_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4449688.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
BASE COUNT          303 a          347 c          234 g          347 t
ORIGIN      
        1 acgcttaatt cattaaacac cactctggcg gtttctgtca tatcctgctg ttttagcatg
       61 tctttttccc ttttccggtt aacgtgacat accaataact cttgtcgaaa aagccagcaa
      121 gttgaaagac cggtattagc aaccaccagc gcgtttaacg ccccgtgccg tttttcagtc
      181 ataaaaaaac ccgcaaaaag cgggctcttt caaatgtcca tgtctgctat tcgcctcgcg
      241 gtacagcttt gcgaagctta ccggaattga agcagttttt acgtcaaaaa gcaataactt
      301 ttttctctat accaaaagcc ataaccattg gtttgtacaa aataaattct gccaccttta
      361 gccaatgctc aatgcgtctt tcacaggttc ttaaactcca ttccggatgt gcatcattca
      421 gcaattcagc cattttgcgc ttagtcatcc cccgccccac ataacgctga ctcagaacat
      481 tgagcagccc gggataacct gccagtactt caccaataac cctgtcgatt attaacgcct
      541 ctgaatcggt acaatgtgcc agccagcttt tttgattgcc gttgatcata tcccgcaaaa
      601 aagcctcaag ttcaggtttg tccagacccg cttttttcat cctccggagc gcctcgttaa
      661 ttgccgtttt tgtcagcttt ttagaggtca gtaattggtt gaacatattt cccgtcttac
      721 cgtcgccaat atacgaccaa cgcccccaca tacgcagttt cccctggatc cagacacttt
      781 ccagcgtttt caggcgtaaa tgctcaccgc ttttgcctgt aatttccggg tatatcatat
      841 ttatgctcac tcactttcaa ttttgtaaat cttcacgccc agccgcccac cagaaacgag
      901 ctggccgcgc acaatattga tttcatcaaa ctgctcgtcg tctatgagaa gtccggcatg
      961 cgtcagcgca tccagtggtg ctttcaggat attgtccagg tcacgacggc gcttatccgg
     1021 tggctctgca ataatcttta tcgccagcct tccggacagg tttaatttca gccgctgctg
     1081 gcgaacaata agtgccacat cccggcgata acgctcaccg gcttttgata caaaatatgt
     1141 gctgccacga cgtcgccagt aggtgttcac cgtcggcggg taaggcaaaa caaactctat
     1201 acgcatcagt aacctctttt acccgagcac g
//
LOCUS       DAZUGK010000451         1226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZUGK010000451 DAZUGK010000000
VERSION     DAZUGK010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.454"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..1206
                     /gene="espG"
                     /locus_tag="ACHY5W_004988"
     CDS             61..1206
                     /gene="espG"
                     /locus_tag="ACHY5W_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFS4449689.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
BASE COUNT          391 a          257 c          238 g          340 t
ORIGIN      
        1 tgatgctata tgatacttgt tgccaaattg ttcattacaa accagatagg agaatctctc
       61 atgataaatg gacttaataa tgactccgca tctttagttt tagatgctgc aatgaaagtt
      121 aattctgggt ttaaaaaaag ctgggatgag atgtcatgcg ctgaaaagtt atttaaagta
      181 cttagttttg gtttatggaa tccaacgtac agtcgtagtg aaagacaatc atttcaagag
      241 ttgttaaccg ttttagagcc tgtatatcca cttcccaatg aattaggcag agtatctgct
      301 cgtttttcag atggttcatc cttaagaatt tccgtcacta acagcgaact tgttgaagcc
      361 gagattcgca cagcaaataa tgaaaagatt actgtgctcc tggagtcaaa cgaacaaaat
      421 aggttattac aatctttacc catcgatcgc cacatgccat acattcaggt tcatcgtgcc
      481 ttatctgaga tggacctgac tgatactacc tcaatgcgca atctacttgg ttttacgtca
      541 aaactatcaa caaccttgat tcctcataat gctcaaacag atccgctttc cgggcctaca
      601 ccattcagct ctatctttat ggatacatgt cgaggactcg gcaatgcaaa gctttcactc
      661 aatggtgttg atatacctgc aaatgcacaa aaattgcttc gcgatgcact aggacttaaa
      721 gacacacatt catcaccaac ccggaatgtt atagatcatg gtatttctcg ccatgatgca
      781 gagcaaatag caagagaaag cagcggcagt gataaacaga aagctgaagt tgtggaattt
      841 ttatgccatc cagaagcagc aacggccata tgctcggctt tctatcaatc tttcaatgtg
      901 ccagccttaa cgttgacaca tgaaaggatc tctaaagcca gtgaatacaa tgcggaaaga
      961 tcgttagata cacctaacgc ttgcattaac atcagtatct ctcaatcatc agatggaaac
     1021 atttatgtta ccagccatac tggggttctg ataatggcgc cagaagaccg ccccaacgag
     1081 atgggcatgt tgacgaacag gacttcttat gaagtgccgc aaggtgtgaa atgtataatc
     1141 gatgaaatgg taagtgcgct acaaccaagg tatgccgcat ctgaaacgta cttacaaaac
     1201 acttaaaata aaactttatt aatcaa
//
LOCUS       DAZUGK010000452         1224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZUGK010000452 DAZUGK010000000
VERSION     DAZUGK010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.455"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..1219
                     /locus_tag="ACHY5W_004989"
     CDS             join(6..297,297..1219)
                     /locus_tag="ACHY5W_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4449690.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    287..403
                     /locus_tag="ACHY5W_004989"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          299 a          295 c          367 g          263 t
ORIGIN      
        1 acaggatgac taaaaatact cgtttttccc ctgaagtccg tcaacgggca gtccgtatgg
       61 ttctggaaag tcagggcgaa tatgactcac aatgggcgac aatttgttcc attgctccaa
      121 agattggctg tacgccggag actctgcgtg tctgggttcg ccagcatgag cgggataccg
      181 ggggcggtga tggagggctc accaccgctg aacgtcagcg tctgaaagag ctggagcgtg
      241 aaaatcgtga actgcgccgc agtaacgata tccttcgcca ggcttccgct tattttgcga
      301 aggcggagtt cgaccgcctc tggaaaaaat gatgccactg ctggataagc tgcgtgagca
      361 gtacggggtc ggaccgctat gcagcgaact gcatattgcc ccgtcaacgt attaccactg
      421 tcagcaacag cgacatcatc cggataaacg cagtgcccgt gcgcagcgcg atgactggct
      481 gaagaaagag atacagcgcg tatacgatga aaatcacaag gtatacggtg tgcgtaaagt
      541 ctggcgtcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg cacgtctcat
      601 ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga ccatcagccg
      661 gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac gacctgacca
      721 gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct atgtggcgtt
      781 catcattgat gtgtttgccg gatacattgt ggggtggcgg gtctcatcgt ccatggagac
      841 gacattcgtg ctggatgcac tggagcaggc gttatgggcc cgtcgaccgt ccggcacggt
      901 ccatcacagt gataaaggtt ctcagtatgt atcgctggcc tacacacagc ggcttaagga
      961 agccggatta ctggcatcaa caggaagtac aggcgactcg tatgacaacg cgatggcgga
     1021 gagcatcaat ggtctttaca aagcggaggt aatacaccgt aagagctgga aaaaccgtgc
     1081 agaagtggaa ctggccacac tcacgtgggt ggactggtat aacaatcgac gattgctgga
     1141 aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
     1201 cgatgatctg gcagcctgag ttca
//
LOCUS       DAZUGK010000453         1222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZUGK010000453 DAZUGK010000000
VERSION     DAZUGK010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.456"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..>1222
                     /gene="nleA"
                     /locus_tag="ACHY5W_004990"
     CDS             1..>1222
                     /gene="nleA"
                     /locus_tag="ACHY5W_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFS4449691.1"
                     /translation="MNIQPTIQSGITTQHNQHHHTEQTPPTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDAYIAPHLEEGSAGKKALDMFGLFNV
                     TQMALEIPSTVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPL
                     QKFLSQNPNPWFGVTAQVVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVI
                     RPGELDLKYGSTPFQSTLGLEFGAHFKWDLDNPNTKYAALTNALANAAGAAGGYTLSQ
                     YTGTNAMLSPHIGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAEFGVAR
                     FTDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNLEDVVIDIESNG
                     SPHHNPSNYVDTFDI"
BASE COUNT          386 a          272 c          259 g          305 t
ORIGIN      
        1 atgaacattc aaccgaccat acaatccgga attaccacac aacacaatca acatcatcac
       61 acagaacaaa cgcccccaac acaaataccg caatccgaat tacctctagg atgccaagct
      121 ggatttgttg ttaatattcc agatgatata cagcaacatg caccggaatg cggtgaaaca
      181 acagctctac tgagcttgat aaaagataaa ggtctgctct cagggttaga cgcatatatt
      241 gctcctcacc ttgaagaagg ctcagcagga aaaaaagcat tggatatgtt tggtttattc
      301 aatgttactc agatggcatt agaaataccc agcaccgttc ctggtatctc tggtaaatat
      361 ggtgttcaga tgaacattgt aaaaccagat attcacccca caaccgggaa ttatttttta
      421 cagttattcc ccctacatga tgaggttgga ataaacttcg gtgcgctccc aggaccactc
      481 cagaagtttc tctctcaaaa tcccaatcca tggtttggag taacagctca agttgttcgt
      541 aaccacggca tagaactacc tatagtcaaa acagataatg gatggaaact agttggagaa
      601 acaccactta cacctgaagg gcctaaagca aattatacgg aagaatgggt catcagacct
      661 ggtgagcttg acttaaagta tggcagcact ccgtttcaga gcactttagg tttagaattt
      721 ggagctcatt ttaaatggga tttagataat cctaatacca aatatgccgc tcttaccaat
      781 gcacttgcga atgcagctgg tgctgcaggg ggatatactc tatctcaata tacaggaaca
      841 aacgcaatgc tgagccccca tattggctcg atggccggac aggctttggg gcatgctgta
      901 caatgctaca ctccaggatt aaagccagac actattttat ggtgggcagg tacgactctg
      961 gggctggcag atttaaacaa ggccgagttt ggagtagcta gattcactga ctatcctcgt
     1021 atatggtggc atgcaagaga aggtgccatc ttccctaata aagaagatat tgcccgtgca
     1081 acaggggctg atatacgagc tatggaagaa ggtatacccg ttggacagcg acatccaaat
     1141 ctagaggatg tggtaatcga tatcgaaagc aatggctcac cacatcataa cccatcaaat
     1201 tatgttgata cctttgatat aa
//
LOCUS       DAZUGK010000454         1219 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZUGK010000454 DAZUGK010000000
VERSION     DAZUGK010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.457"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1143
                     /locus_tag="ACHY5W_004991"
     CDS             <1..1143
                     /locus_tag="ACHY5W_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4449692.1"
                     /translation="DEVSEKLEYIPAQVVVNRYIRPQYSCPCSEKVFSGKMPAHILPK
                     SAVEPSVIAQVVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAPLAKL
                     LRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLWAYVSGERSGPPVVCFDSQTGRAL
                     RYPEAWLQGWHGGTLVSDGYNVYKSLADNHPGITSACCWSHARRGFANLYKASREPRA
                     AMALRKIAGLYRIEKLIRERPVEKIRQWRQRYSRPIVNDLFAWLEEQEPCCPPEGPLN
                     KAINYILNRRDELSCFLGDGAVPLDNNICERAIRPVVMGRKAWLFAGSLMAGNRAAQI
                     MSLLETAKRNGLEPHAWLTDVLTRLPEWPEDRLEELLPLEGFTFSG"
BASE COUNT          269 a          325 c          371 g          254 t
ORIGIN      
        1 gatgaagtca gcgagaagct ggaatatatt ccggcacagg tcgtggtcaa ccgctatatc
       61 cgcccgcagt acagctgtcc ctgcagtgag aaagtgttca gcggaaaaat gccggcacat
      121 atccttccga aaagtgctgt tgagccatcg gtcatcgcac aggttgtcat cagcaaatat
      181 accgaccatc tgcccctgta tcgccagcag cacattttct cccgaatggg ggtggagctc
      241 cccgtcagta caatggcgga tatggtcggt gttgctggcg ctgcactggc cccgctggca
      301 aaattgttac gccacgagct tctgacacgg gatgttattc atgcagatga aacgtccctg
      361 aggctccttg atacccggaa aggcggtaaa tcctgctccg gctggctgtg ggcttacgtc
      421 agcggagaaa gaagcggtcc gccggtcgtc tgttttgact cgcagaccgg gcgggccctc
      481 agatatcctg aggcctggtt gcagggctgg catggcggta cgctggtgag tgacgggtac
      541 aacgtgtaca aatctctggc ggataatcac ccaggtatca catctgcctg ttgctggagc
      601 cacgcaaggc gcggcttcgc aaatctgtac aaggccagca gggagccccg tgcagccatg
      661 gcgctgagaa aaatcgccgg cctttaccgc attgagaagc tcatccgtga gcgtcctgtg
      721 gaaaaaatac ggcaatggcg acaacggtat tcccggccga tagtgaacga cctgtttgcc
      781 tggcttgaag agcaggagcc gtgttgtccg cctgagggcc cattaaataa ggcgattaac
      841 tatattctga accggcgcga tgaactgagt tgcttcctgg gggacggagc tgtgcccctg
      901 gacaacaata tctgcgagcg tgccatccgc ccggtggtca tggggcggaa agcatggtta
      961 ttcgccggtt cactgatggc cgggaaccgc gcggcacaga taatgagcct tctggaaacg
     1021 gcaaaacgta atggtctgga gccgcatgcc tggctgacag acgttctgac gcgtctaccg
     1081 gagtggccgg aagaccgact ggaagagttg ctgccccttg agggatttac cttctccggg
     1141 tgagtgatac ctgccatcag gtgttcgtgc agcgggccat aacccgcagt cgggagttga
     1201 acgcctgacg acaggaaat
//
LOCUS       DAZUGK010000455         1216 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZUGK010000455 DAZUGK010000000
VERSION     DAZUGK010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.458"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..602)
                     /locus_tag="ACHY5W_004992"
                     /pseudo
     CDS             complement(1..602)
                     /locus_tag="ACHY5W_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
     gene            complement(766..876)
                     /locus_tag="ACHY5W_004993"
                     /pseudo
     CDS             complement(766..876)
                     /locus_tag="ACHY5W_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(923..1210)
                     /gene="nleC"
                     /locus_tag="ACHY5W_004994"
                     /pseudo
     CDS             complement(923..1210)
                     /gene="nleC"
                     /locus_tag="ACHY5W_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
BASE COUNT          350 a          291 c          223 g          352 t
ORIGIN      
        1 catatatttt ctctgcactc ccggcaccat aatactggtt gaaacaacga acttcgcttg
       61 tcacctcttc atggctttga gatatagtaa acatcttcaa cacttttgtt gtattttcca
      121 tatcttcata cacgacagca ttaccacctt taccaataac gttacctagc acgggtgatt
      181 tgttgttgct aagcctcagc aacgctgact gagtataatc cgctggcggt aactccggct
      241 gcgcataatc cactggaggt aaatcaggtc tgttcgaacg aacagatcct ctttctatag
      301 tcgtgctcac tgtcgatgaa ttcagcatag cctcaatttt tctggtgatc tctcccttag
      361 gccatcccag tctatacagc atattagtaa aacatccact atgactctct cttgtcacgc
      421 aaaacctatt atcacttaaa acaacgcgat atgttctgtt gctaaccttt acttgcgccc
      481 cattatcaga gtgagcggca gcatccctta cagagggata aaacacgatt atcaggcaaa
      541 gtcaggtttc tggttaaaga attccatgaa caccccaaat ttacagaaga tggtgatagc
      601 atacatttca accttcaaaa tgaatcaatc tttactttct taacaaacat caccatgaca
      661 tgacaacaaa aaactggaaa ttaactattg tttttgagta tctggcaact cattcatcct
      721 tccagcatcc cgtcagacga acgtttacga actataaatc aggtattact ggccctcgaa
      781 atctggagat actgacgtat caacacggtt cagtaacacc cgatacctct tccagactgt
      841 caacaacgat gtctcttcat ccgttgcgat ctgcagcatc ctgaatatga atgccatagc
      901 ttacggcatc acgaaaagtt tatgagcggc tcaaagcatc aagtatagtg ttgccgatca
      961 tatcgactgt atgctgatcg ataagcctgc tacggctgtc atggacggcg cgtattacac
     1021 tttcgtatac gttgcttaaa tctgctgcgg aaagtggtat tcgcttacct atatccaaaa
     1081 cgtaatccgc ataggcattt tcagcacgat taggagcaat gggagcagag tatcctgccg
     1141 gggcggaaaa gttgaagttg gactgtaatg agggaatttt catgtttata tctaataccc
     1201 tgcaaatcat ttatta
//
LOCUS       DAZUGK010000456         1214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZUGK010000456 DAZUGK010000000
VERSION     DAZUGK010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.459"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..316
                     /locus_tag="ACHY5W_004995"
     CDS             <1..316
                     /locus_tag="ACHY5W_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449693.1"
                     /translation="SEFKRLRDFQEYVKKACVSLSFIQAVANGSKHRGINRYKPAVRS
                     TERKNGAFSSGFSNDFDISHLVMEIEDGKFVYFQEEINKALSFLKSYLNGLTNNPLIN
                     KE"
     gene            complement(606..845)
                     /locus_tag="ACHY5W_004996"
                     /pseudo
     CDS             complement(606..845)
                     /locus_tag="ACHY5W_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(1101..1214)
                     /locus_tag="ACHY5W_004997"
                     /pseudo
     CDS             complement(1101..1214)
                     /locus_tag="ACHY5W_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          324 a          278 c          256 g          356 t
ORIGIN      
        1 ctcagagttc aaaagattaa gagactttca agaatatgta aaaaaagcat gtgtttcgct
       61 atcttttatt caagcggtgg ctaatgggag taagcataga ggaatcaatc gatataagcc
      121 agcggtgagg tcaactgagc gaaaaaatgg agcattttca tcaggttttt cgaacgattt
      181 tgatatctcc catttagtaa tggaaataga ggatggaaag tttgtatact ttcaagaaga
      241 aataaataaa gcattatcct ttttgaaaag ttatttaaat ggattaacaa ataatccatt
      301 gattaataaa gagtaattct atcatcgtca gattttgggc acagagcaga cagttagact
      361 ggtttggctc tgtgccatct atgtgtcagc tcatatcgga gctggtttat gttactcaat
      421 aactcccgca aacctgtaaa tcttgcgtga tgcccattta tttgggcagg atttaatatc
      481 aggatctgga aagtctggcc tgtatttctg gccagttctc ctgtttacgc tgttccagcg
      541 aagaactgtc gatactgaaa ctccacagaa gttggcgact tgtttagttg tcattaagtt
      601 gttcattact ttacctcctg cggcggctcc ggtagcggca tccagtgggt tactttcgat
      661 gccggttctt ccacatcgtc agtaactgcc caccatttgt ttctcgacca atcgtaatac
      721 ccttcgaagg tatcgcactc agtccagccg taagacttcc cccaacacca aacatactgt
      781 ttatcgtccg gcattcgctc actacagctt atccaaccat ccggagttac cggagagttg
      841 cccgacagct cgttcaactt gtaagtctgg cttacaggtt cggtaccatg aagcatggcg
      901 gcgcggcagg cgttccagcc ttcatcaaag ccgactatgc cattatttaa agacggacga
      961 acatctggca ccatcggcac tggcttggct atatatagcg gctgaacata ccagcccttt
     1021 gataaccaac ggtcagcaat gtttttactc cgtgttattg ccggaatacc taagccattg
     1081 tctgaatgca gccatgccac cggttctgct tccagcgata ccaaagcaat ttcataagca
     1141 cggcgctcaa tattgtctcg cacatccaga ctgcctatgc gctctttgat ttctttaatc
     1201 agttctttgt cggt
//
LOCUS       DAZUGK010000457         1209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZUGK010000457 DAZUGK010000000
VERSION     DAZUGK010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.460"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..602
                     /locus_tag="ACHY5W_004998"
     CDS             72..602
                     /locus_tag="ACHY5W_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS4449694.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVPPFVTPNSEIKALIKNIHETWANVGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          360 a          255 c          248 g          346 t
ORIGIN      
        1 accacatctg tataaggaat ttttaaggtt cgtgggatag cttgactgtg aaaatcacag
       61 gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta tttacattgg ataacgctgc
      121 ttttcgttgc aataacctat gccgcgatgg aactccgtgg ctggtttcct aaaggtagta
      181 gtacttatct gctgatgcga gaaacacatt acaatgcggg tatattcgtt tgggtgttaa
      241 tgttttcacg cctgattata aaacaccgtt atagtgatcc ttctattgtg ccaccgccac
      301 ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat gctttatata accttccttg
      361 cgcttcctct gctggggatt gctttaatgg cttacagtgg aaaatcgtgg agtttccttg
      421 gtttcaatgt gcctcccttt gttaccccaa acagtgaaat taaagcactg ataaaaaata
      481 ttcacgaaac ctgggcgaat gtaggttact ttttaatcgc agctcacgct ggcgcagcac
      541 tctttcatca ctacattcag aaagataata ctctgttacg aatgatgcct cgccgcaaat
      601 aagtcgatcc cctgacaagg aaatgtgaac cctggacatc aacgcatcat aaatgtttac
      661 gcaagtgcat aatgcactct ttccactatc aacaaggagg agcagaaact gtgtaaatcg
      721 tacaatggta agacttatca aaaatacagg ttaattttgg tatttaaacc ttttttactt
      781 gttagtatga tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaaattat
      841 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttgactc aattattgac
      901 cccttctcta cgcaacttca gttcccacca ccaactttgc ggcagtttca taggatcaat
      961 gtctaaaaga ataatggtga gcgataagaa aacgactgaa taactgcaca ttttcgctcg
     1021 aaagcttcct gtcagatcca tagcgaatca agtgctgaat gtcacagtat cgaacagaaa
     1081 acagtgacga tctaaccctt caagaatatt ctacgattgt tctgtttagg aaaagcaagg
     1141 cgggaagtcg ggagataagt cattgataaa gtggcggaga gagggggatt tgaacccccg
     1201 gtagagttg
//
LOCUS       DAZUGK010000458         1202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZUGK010000458 DAZUGK010000000
VERSION     DAZUGK010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.461"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            206..1165
                     /locus_tag="ACHY5W_004999"
     CDS             206..1165
                     /locus_tag="ACHY5W_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFS4449695.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          437 a          229 c          214 g          322 t
ORIGIN      
        1 taaaaattat aaaaaacata aaacaccacg caaacacact taataaaaac accattacat
       61 taaaagataa taaaaccgca ataaaaaacg aataaatcaa ttgtctcacg caattataaa
      121 acatcatatt gattacgcac cttgtattac aaactcatgt atgtaaaata cgcgcaccat
      181 tcaaaaaaaa ggaagacaat aacatatgaa aaaaagtgtc atcgctggcg tctttattgc
      241 tctgtcattt accacgtgtt cagctatcgc gaacagcctt gcattatcat tagcaaatga
      301 tgatgcaggg aagtttcaac caatacttaa tgatatttat ggcaataaac atgaaaacag
      361 agatgattac tcacaaggct tatttctggg atatagccac gatatctcag actcgagcca
      421 attatctctc catattgcgc aagatattta ctctccatca ggcagtaata aaagacacaa
      481 cacagctgta actggagaca gagcttttag tgcatacact cacactggta ttgaatggaa
      541 ctcccttgcg aatgactgga ttcgctatcg attaggtact gacataggtg ttgttggccc
      601 cgacgcaggc ggtcagaaag tacaaaataa agctcatgag attattgggg cagaaaaata
      661 tcatgcatgg gatgatcaaa tagagaatcg ctacggttat actgtaaaag ggatgctatc
      721 catgacacca agtatggata ttttaggtgc taatgttgga ttataccctg aagtttctgc
      781 tgttactgga aacttatttc aatatgtagc atatggcgca accattgcca ttggtaatga
      841 taaaaccttc aattcggata atggctttgg tctgctggct ccccgtggtt taatgcatat
      901 gtccgataca agcggattca aatacaagat ttttgcaggt atggaaagac gagatgtcaa
      961 tcgcaactat actctcgaag gaaaaacaat acagacgaaa caaacaacag tatcgctaaa
     1021 caaaactgtt gatgaatatc aagttggcgc aacaattggg tatgcacctg tagccttcac
     1081 actagcattt aataaagtaa catcagaatt caagacaggg gatgactatt catttataaa
     1141 tggagcaatc accttctttt tttaactgaa ttgaattcaa tcaaaataac ataagtccaa
     1201 ca
//
LOCUS       DAZUGK010000459         1200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZUGK010000459 DAZUGK010000000
VERSION     DAZUGK010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.462"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1200
                     /locus_tag="ACHY5W_005000"
     CDS             <1..>1200
                     /locus_tag="ACHY5W_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFS4449696.1"
                     /translation="KAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPLS
                     LLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAVL
                     AALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLA
                     RLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNF
                     LSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWT
                     SDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVG
                     ALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQ
                     EAVSRLA"
BASE COUNT          278 a          286 c          400 g          236 t
ORIGIN      
        1 ataaggctgc catgcgcaca ctgcccgcac agttcacgga tattgtcact cagcttgccg
       61 gtggtcagaa tcccttcctt atcatgctgc agcagggggg gcagatcagc gattcattcg
      121 gtggaccgct cagcctgctt accctgctga aggaggaact tctcgggatc agggatgcct
      181 ctgaatcatc agaggagtcg ctgtcagata cggcaaatgc actggctgaa aatgcccgga
      241 atgccggtga gctgggacga tttatgtcgg tggcccgtgt ggcggcaggt ggcggggttg
      301 ccgtactggc cgcgcttgct gccgccgcct ggcaggcaga gcaggctgac cgggccttat
      361 tgcgttcact gatcctgacc ggaggggcgg ctgccaccac aacggcagaa ttgtggaaaa
      421 tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca gaaaatctgg
      481 cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg ggggaaacct
      541 ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa gcctttgaag
      601 ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat aacttcctga
      661 gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa caggctgcgg
      721 tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag caacttgata
      781 aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact tctgacgcat
      841 gggcatggat aggtgatcat acactggggg cactcagtct gatcactgac gtggtggccg
      901 gaaccgttga acaagtgaag ctgctgcttg tgcaggggga tctggcgctg gctgaattta
      961 ttcagtcagc ctgggaaacg acaaagaatg tgcccggcgt tggtgcgttg tttggtgaac
     1021 tggcagaaga gaaccgcgta tttattgaga aaacaaaacg cgatgaactg gcgctgagaa
     1081 aatccattgc ggaacgggat gcgcgtatac gccaggggga aatggggtac atcaaccgct
     1141 cgcgtgcaac aggcgtcagc aaaggtccag ggcagcagga agccgtcagc cgtctggctg
//
LOCUS       DAZUGK010000460         1199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZUGK010000460 DAZUGK010000000
VERSION     DAZUGK010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.463"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..436
                     /gene="escQ"
                     /locus_tag="ACHY5W_005001"
     CDS             <1..436
                     /gene="escQ"
                     /locus_tag="ACHY5W_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFS4449697.1"
                     /translation="ALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVIN
                     TPLATVNLSDNNLSVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDI
                     INSDGYHLFGGCQLIRNNKTIAYGSIVKINEDFYFTVSIVCD"
     gene            467..973
                     /gene="espH"
                     /locus_tag="ACHY5W_005002"
     CDS             467..973
                     /gene="espH"
                     /locus_tag="ACHY5W_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFS4449698.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
BASE COUNT          361 a          200 c          260 g          378 t
ORIGIN      
        1 tgctcttttt tttgatggct atatcgtacc tgggtggact gattatccag ttacagagtt
       61 gagagttggt gatagtctga gactctatca tgttgatgat agtcaagaga gaaggtgctg
      121 gattgttatt aatacccctt tagcaacagt aaatctgagt gataataatt tatctgtcac
      181 ggatgttctg gctgcggatc tgttatctgc tcttagcaat gagactgtca tgaatcgcat
      241 ttattgtgcg atcggtacag tccatattga tatacacatg ttgcgtaatg ttaaaaaaga
      301 tgacattatt aattcagatg gataccatct ttttggtggt tgtcagttaa tacgcaataa
      361 taagacgata gcatacggga gtattgtaaa aatcaacgaa gatttttatt ttactgttag
      421 catagtatgt gattaatcag gaatacgtta tatagggaga tgtatcatgt cgttatcagg
      481 agcggtattc aagacatttc tgacaagtga acatgcgtca tggaacaggt tcaacagaag
      541 gcttcatata cccaatgaag atatcgtaga tgaaattcag cttaaagccc gaatgcaaca
      601 acgccatcat cgggtatatc cagaaatagg ggattccacc attgtctctt ttagaggcaa
      661 agactatgct gttcacttta ttaaagatgg cccaaaagat gattatgtgt acaaagtaca
      721 aagaataaca ccggaaaatg gatgtttctc aacgctattt agcgtgtttt ctggcggtgt
      781 gactaaggct ttggaaagaa aattaaatga gagacatata actccacttt cttcaacctg
      841 gtttcctcgt actcccctgg aggggatctt agctgaaaga ggattatctt ctttgttacg
      901 tcgtgtacag tctaccgaac gtcttgataa tagggctata gcaacaagag cttcctctta
      961 tagcgtatta tgattagcat ttgagttttc ttcctgactt aactccagaa tagttataat
     1021 tctggagttt ttccgctttt taactgatat tacattgctt ttagataata caggctgaat
     1081 cttcagagac gttagcggcc agaaatcaga ggtccctgtg atgaagagta tgttaaacgg
     1141 aatggatgat cccatccagc gcagaaatta tcaaggtaaa aaatctgtag gtctggaag
//
LOCUS       DAZUGK010000461         1189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZUGK010000461 DAZUGK010000000
VERSION     DAZUGK010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.464"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..1016
                     /gene="fnlA"
                     /locus_tag="ACHY5W_005003"
     CDS             3..1016
                     /gene="fnlA"
                     /locus_tag="ACHY5W_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFS4449699.1"
                     /translation="MFKDKILLITGGTGSFGNAVLNRFLSTDVKEIRIFSRDEKKQDD
                     MRKNYNNKKLKFYIGDVRDYRSILNATRGVDFIYHAAALKQVPSCEFHPVEAVKTNVL
                     GTENVLEAAIANEVKRVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDCNKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIETLATALKDLLNAAHHPVNIIGTRHGEKLFEALLSREEMLSAEDM
                     GDYYRIPPDLRDLNYGKYVEQGDSRISEVEDYNSHNTQRLNVEGMKTLLLKLDFIRAL
                     HAG"
     gene            1039..>1189
                     /locus_tag="ACHY5W_005004"
     CDS             1039..>1189
                     /locus_tag="ACHY5W_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4449700.1"
                     /translation="MKILITGANGFIGRNLSLRLEELGYKNLIRIDRDSTEQDLEQGL
                     QDADFI"
BASE COUNT          376 a          185 c          252 g          376 t
ORIGIN      
        1 tgatgttcaa agataaaata ctgttaatca ctggtggtac tggttcattt gggaatgctg
       61 tattaaatcg ctttctttcc acagatgtaa aagaaatacg catatttagt cgtgatgaaa
      121 aaaagcaaga tgatatgcgt aaaaattata acaataaaaa attgaaattc tatattggtg
      181 atgttagaga ttatcgtagt attttgaatg cgactcgcgg agttgatttt atatatcacg
      241 cagcggcact taagcaagtt ccatcatgtg agtttcatcc tgtggaggct gttaaaacta
      301 atgttcttgg tacagaaaat gtgcttgaag ctgctatagc gaatgaagtt aaaagggttg
      361 tgtgtcttag tactgataaa gctgtatatc cgatcaacgc tatgggaatt tcaaaagcta
      421 tgatggaaaa agtcatggtt gcgaaatcgc gtaatgttga ctgcaacaaa acagtaatat
      481 gtggtacccg ttatgggaat gtcatggcct ctcgtggatc tgttattcca ttatttgttg
      541 atcttatcaa atctggcaag ccactaacaa ttactgaccc taacatgacc cgttttatga
      601 tgactcttga ggatgctgta gatttagtac tttatgcatt cgaacatggt aataacggag
      661 atatttttgt acaaaaagct ccagctgcaa ctatcgaaac attagcaact gctttaaagg
      721 atttactaaa tgctgctcat cacccagtaa atattattgg tacgcgtcat ggtgagaagt
      781 tatttgaagc tttgctcagt cgtgaggaaa tgctctctgc tgaagatatg ggggattatt
      841 atcgtatacc accagatctt cgtgacctta attatggaaa atatgttgaa caaggtgata
      901 gtcgtatatc cgaggttgaa gattacaact ctcataatac acaacggttg aacgttgaag
      961 gcatgaaaac gcttttgctc aaattggatt ttattcgtgc acttcatgca ggttaacaac
     1021 atggctaaga ctcatgatat aaaaatattg attactggtg caaatggttt tattggccgt
     1081 aatttaagtt tgaggcttga ggaactaggt tacaagaatc ttattagaat tgatcgggat
     1141 tcaacagaac aagatcttga acaaggctta caggacgctg atttcattt
//
LOCUS       DAZUGK010000462         1185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZUGK010000462 DAZUGK010000000
VERSION     DAZUGK010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.465"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..259
                     /locus_tag="ACHY5W_005005"
     CDS             35..259
                     /locus_tag="ACHY5W_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4449701.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQRTCSDRFT"
     gene            complement(341..655)
                     /locus_tag="ACHY5W_005006"
     CDS             complement(341..655)
                     /locus_tag="ACHY5W_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS4449702.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(1121..>1185)
                     /locus_tag="ACHY5W_005007"
     CDS             complement(1121..>1185)
                     /locus_tag="ACHY5W_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4449703.1"
                     /translation="MAMQKQLEGAQEYIRTQCIP"
BASE COUNT          372 a          293 c          176 g          344 t
ORIGIN      
        1 tacaaatcag caacactgaa tatggggcaa cattatgtca tcaaagaaca gaacccgcag
       61 aacaacaacc cgcaacatcc gatttccaaa ccagatgatt gaacaaatta acatcgctct
      121 tgatctgaaa ggttcaggaa atttttcagc gtgggttatt gaagcctgca gaagaagatt
      181 aattaatgaa aaatattctc aatttgtacc caacaaagac aaacacgacc agagaacctg
      241 ttcagacagg tttacttaaa cgacttatat atgacacaaa aagcgaccac taaagtcgct
      301 ttttcttatg gtaacaggca ataacgctct cagatatttt ttagcatttt tttgaccgcg
      361 cgtttccgga cgtattctgt tctcctgtcc ctttatatcg tcggaatacc cgccgctctt
      421 caaatcccat tcccaactca gaatgtagtc tgttgaccgc ttgttttatt tcggtcaggt
      481 tcaccggtga aaccggagtc cggcgcgcct tacgcaaaca ctctgctcgt ttctgtgccg
      541 ccacttttct tttctggtca tcacttagct gtaccatcac ttttgcccat cgttcagctg
      601 ctctccggta cagtcctttt ttctccagac attctgccac gtgatcatgt agcataagtg
      661 acctccgatt atctacagac tgccatcctg aatttacctt cccttaatga aataacaata
      721 aaaaacaaac cacgcaaaaa caataaaaca acacacaaaa aaaactaaat aataaacaaa
      781 aataatcacc ttattttatt attttttgag ggagcaatta ctgaacaaaa aacgctgact
      841 atatactcaa aaccaaacaa ctattctgcc aatcaggtat catggcaaca cacggaatta
      901 ccgtgttttt gccttctctg cccatacaat acgggcatat acttcattct ctattgtaat
      961 atttctatcc atgtgcccca ctccatttac ctgtaaataa tattcaaaat atttatcaca
     1021 gaaatcgttt ttggccatga actgagcaca ctataaagtc cggaactgac tctttgttaa
     1081 attaccttaa cgttaccagt aacaccttca taacaaaaca tcacggtata cactgggtac
     1141 ggatatattc ctgtgctcct tccagttgct tctgcattgc catca
//
LOCUS       DAZUGK010000463         1169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZUGK010000463 DAZUGK010000000
VERSION     DAZUGK010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.466"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..736
                     /locus_tag="ACHY5W_005008"
     CDS             <1..736
                     /locus_tag="ACHY5W_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFS4449704.1"
                     /translation="LGADWSGTIKIGSSLSASFNNSGSISTLDGTQFIATIQALAQKR
                     RAAVVARPVVLTQENIPAIFDNNRTFYTKLVGERTAELDEVTYGTMISVLPRFAARNQ
                     IELLLNIEDGNEINSDKTNVDDLPQVGRTLISTIARVPQGKSLLIGGYTRDTNTYESR
                     KIPILGSIPFIGKLFGYEGTNANNIVRVFLIEPREIDERMMNNANEAAVDARAITQQM
                     AKNKEINDELLQKWIKTYLNREVVGG"
     gene            741..>1169
                     /locus_tag="ACHY5W_005009"
     CDS             741..>1169
                     /locus_tag="ACHY5W_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HFS4449705.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLN"
BASE COUNT          396 a          202 c          271 g          300 t
ORIGIN      
        1 gctaggtgct gactggtcgg gcacgataaa aattggatcc agcttaagtg catcatttaa
       61 taattcaggt tcaattagta cccttgatgg gacacagttt attgcaacaa tacaggcact
      121 tgcacaaaaa agaagagccg ccgttgttgc tcgtcccgtc gttttaacac aggagaatat
      181 tcctgctata tttgataata accgtacatt ctatactaaa ttagtcggcg agcgtactgc
      241 agaattggat gaagtaacct atggtacgat gattagcgtg ctaccacgat ttgctgcgcg
      301 aaatcaaatt gaattactct tgaatattga agatggcaat gaaataaatt ctgacaagac
      361 caatgttgat gatctgcctc aggttggtag aacgcttata agtactattg cacgtgtgcc
      421 ccaagggaaa agtcttttga ttgggggata tacacgcgat acgaatacct atgaaagtcg
      481 caagatccca atattaggca gcattccatt tattggtaaa ttatttggtt atgaaggaac
      541 aaatgcgaat aacatcgttc gtgtctttct tattgagcca agagagatcg atgagcgcat
      601 gatgaataat gcgaatgagg ctgcggttga tgccagagcg attacacagc aaatggcaaa
      661 aaataaagaa attaacgatg aattactgca gaaatggata aaaacttacc tgaatcgtga
      721 agtcgtcgga ggatagtgaa atggcgattc atgtagagca tgtcggtgta ttagaaagag
      781 ccagggaagt ttctcgctta gaggacatta tcacagaaga taatgaagat attgaagctg
      841 aaatgcctaa aatgggggat gatcccgcag gtaaagaagc tcggttctta caagctactg
      901 acgagatgtc agcagcgtta acgcagttta tgaagaaaaa aatctacgaa gaacaactgg
      961 ccaattttct ggacggagaa gaatatgtcc tggaagacca gccaatagaa aaaaccgaca
     1021 aagttatgga ggcgcttaag gctgcaacta cccatgacta tgaagtgtat agctttgcaa
     1081 aaaaattatt cccagatgaa agcgatctgg tagttgtgtt acgtgcaatt ttacgtaaaa
     1141 aacaaatatc agaaaatgtc cggctaaac
//
LOCUS       DAZUGK010000464         1163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZUGK010000464 DAZUGK010000000
VERSION     DAZUGK010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.467"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..953
                     /gene="gadX"
                     /locus_tag="ACHY5W_005010"
     CDS             129..953
                     /gene="gadX"
                     /locus_tag="ACHY5W_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFS4449706.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLSNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          346 a          216 c          243 g          358 t
ORIGIN      
        1 gtagaacatc acacattatc atcctgttct cccgcttcgt ttaaatttat ttatcaatca
       61 atttgactta agagggcggc gtgctacatt aataaacagt aatatgttta tgtaatatta
      121 agccaactat gcaatcacta catgggaatt gtctaattgc gtacgcaaga cataaatata
      181 ttctcaccat ggttaatggt gaatatcgct attttaatgg cggtgacttg gtttttgcgg
      241 atgcaagcca aattcgagta gataagtgtg ttgaaaattt tgtatttgtg tcaagggaca
      301 cactttcatt atttctcccg atgctcaagg aggaggcatt aaatcttcat gcacataaaa
      361 aagtttcttc attactcgtt catcactgta gcagagatat tcctgttttt caggaagttg
      421 cgcaactatc gcagaataag aatcttcgct atgcagaaat gctacgtaaa agagcattaa
      481 tctttgcgtt gttatctgtt tttcttgagg atgagcactt tataccgctg cttctgaacg
      541 ttttacaacc gaacatgcga acacgagttt gtacggttat caataataat atcgcccatg
      601 agtggacact agcccgaatc gccagcgagc tgttgatgag tccaagtctg ttaaagaaaa
      661 aattgcgcga agaagagaca tcatattcac agttgcttac tgagtgtaga atgcaacgtg
      721 ctttgcaact tattgttata catggttttt caattaagcg agttgcagta tcctgtggat
      781 atcacagcgt gtcgtatttc atttacgtct ttcgaaatta ttatgggatg acgcccacag
      841 agtatcagga gcgatcggcg cagagattgt cgaaccgtga ctcggcggca agtattgttg
      901 cgcaagggaa tttttacggc actgactgtt ctgcggaagg aataagatta tagaatttta
      961 ctcagacata aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag
     1021 actaataaac cgttataaca ctccctgttg gcacgggaaa ttttgtgctc tcagtaagtt
     1081 aaatataact ttcactggaa ataagatcag ccattttttt ataaacataa gctatacgct
     1141 gtgcgaaaat atattcgtgt tgc
//
LOCUS       DAZUGK010000465         1146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZUGK010000465 DAZUGK010000000
VERSION     DAZUGK010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.468"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..738
                     /gene="sdiA"
                     /locus_tag="ACHY5W_005011"
     CDS             16..738
                     /gene="sdiA"
                     /locus_tag="ACHY5W_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFS4449707.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYREIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(806..1030)
                     /gene="yecF"
                     /locus_tag="ACHY5W_005012"
     CDS             complement(806..1030)
                     /gene="yecF"
                     /locus_tag="ACHY5W_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFS4449708.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDKTQAAVFSKTVKQIKQAYRQ"
     gene            complement(1017..>1146)
                     /gene="yecU"
                     /locus_tag="ACHY5W_005013"
     CDS             complement(1017..>1146)
                     /gene="yecU"
                     /locus_tag="ACHY5W_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFS4449709.1"
                     /translation="VDITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          294 a          251 c          283 g          318 t
ORIGIN      
        1 gcgttgcggt ttactatgca ggatacggat tttttcagct ggcgtcgcac gatgctgttg
       61 cgttttcaga ggatggaggc cgcagaagag gtctaccgtg aaatagagct tcaggctcag
      121 cagctggagt acgattacta ttcgttatgt gttcgccacc cggtaccatt cacgcgacct
      181 aaagtggctt tctacaccaa ttaccctgag gcgtgggtta gttattatca ggcaaaaaac
      241 tttctcgcaa ttgatccggt gctgaaccct gaaaacttta gtcagggcca tttaatgtgg
      301 aatgatgact tattcagcga agcacagccg ttatgggaag ccgcgcgcgc acatggttta
      361 cgccgcggtg tcactcagta tttaatgctg ccaaaccggg cgctgggctt tttgtccttt
      421 tcccgttgca gcgcgcgcga aatacccatt cttagtgatg aactgcaatt aaaaatgcag
      481 ttactggtgc gcgaaagtct gatggctctg atgcgtttaa atgatgaaat agtgatgacg
      541 ccagagatga atttcagcaa gcgcgaaaaa gaaattctga ggtggacggc ggaagggaag
      601 acatcagcag agatagcgat gattttgtca atctctgaga atacggtcaa tttccaccag
      661 aaaaacatgc agaaaaaaat taatgcacca aataagaccc aggttgcctg ttacgcggcc
      721 gctactggct taatttgatc tcttttctgt cctgcgtgcc agatgcaaaa accggctgaa
      781 aggcacgcta tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
      841 ttttggaaaa tactgcagcc tgggttttgt cttcgatcag cgcaagctgt ttttccatct
      901 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg cagcatcagc gtcagcatcg
      961 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     1021 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     1081 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgata
     1141 tcaaca
//
LOCUS       DAZUGK010000466         1143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZUGK010000466 DAZUGK010000000
VERSION     DAZUGK010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.469"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..224
                     /gene="yniD"
                     /locus_tag="ACHY5W_005014"
     CDS             117..224
                     /gene="yniD"
                     /locus_tag="ACHY5W_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFS4449710.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(277..1035)
                     /locus_tag="ACHY5W_005015"
     CDS             complement(277..1035)
                     /locus_tag="ACHY5W_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFS4449711.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPAESAPEHTDRRTTLSLGYSM"
BASE COUNT          318 a          282 c          270 g          273 t
ORIGIN      
        1 aaacagtgat caggtgtaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg
       61 cctgtccctt ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc
      121 cgactaaacg ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg
      181 gcgctatgtt gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaaa
      241 gcagccagac cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa
      301 ggttgtccga cggtcggtat gttccggtgc agactctgca ggttcagagt tccatgtgac
      361 gttgtaagcc accttcagtc caaaatgttc attaatagca acatttaatg cactttcgga
      421 gttcagcgtt gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc
      481 agtcaactgc catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt
      541 gtcggtatat ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg
      601 gccattcaaa aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt
      661 ataacggtct gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa
      721 acggctacgc ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt
      781 actggcgttc ccccacagcg accaggcagt agtttgtcca taccaggtca tggtggtgtc
      841 agccgtaagt gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt
      901 accttcgaaa ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc
      961 ggcagctgca ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt
     1021 cttcaaaagc ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg
     1081 gaaactttct taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc
     1141 gcg
//
LOCUS       DAZUGK010000467         1125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZUGK010000467 DAZUGK010000000
VERSION     DAZUGK010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.470"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(136..921)
                     /gene="ygcW"
                     /locus_tag="ACHY5W_005016"
     CDS             complement(136..921)
                     /gene="ygcW"
                     /locus_tag="ACHY5W_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4449712.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIHVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT          339 a          261 c          222 g          303 t
ORIGIN      
        1 aattacgcga tctgcaccaa aaatttcctt tagctggtcg acaatcgctg cgcgagataa
       61 agacatagta attccttcct gataataaaa atggaataat cattaataag agaaaacaga
      121 aaccaccgtt atgggttagc gcactaaata accgccatca accactaata aatgcccgtt
      181 gacataattc gatgccggac ttgcgaggaa tacagctgcg cccattaaat cctgagtatc
      241 gccccaacgg tttgccggaa tatgatcaag aacgcgctga ttggtttctg gattactgcg
      301 tgtcgccagc gtaatatctg ttgcataata gccaggggcg ataccattta catgaatatt
      361 atattgccct aattcatcac aataagcttt ggtgaatccg gcaagagcat gtttagtggc
      421 agaatatgcg ggtgaccatt gtccacctaa gtaagagaac aatgaacaga tattaataat
      481 tttaccgctt ttctgcggga tcataatttt tgccgcttcg tagcttaact cgaatgcggc
      541 ggtcaggttc acatcaatca tcgggtccca gtcggctcga ccaaagtcca gcaccttatt
      601 tagcttacaa ataccggcat tgttaaccag aatatcaact gttccgaaac gctcacagca
      661 agcagcgatt atcttctgcg gcgcgccttc tgcggtgata tccacctgca tgaagtccac
      721 ctcaacaccc tgattttcaa tcatttcctt tgtttcgccg ttatctttga caaaacttgg
      781 aataaagaca tttgcgcctg ctttagccaa cgccatggca aatgcctggc ctaaaccgct
      841 attcccgccg gtaacaattg cggttttacc tttcagggag aaaaaatcca ttgagaacgc
      901 attgagagat tcgattgaca tagttagctc caatctccgt aaggcaaaaa aaaagaaagg
      961 taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact ggagataatt
     1021 tttagcataa ggacataact gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc
     1081 aaataatttt aaaaagcaga agcgtgatta ctctcacaca acacc
//
LOCUS       DAZUGK010000468         1121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZUGK010000468 DAZUGK010000000
VERSION     DAZUGK010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.471"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..531
                     /locus_tag="ACHY5W_005017"
     CDS             <1..531
                     /locus_tag="ACHY5W_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS4449713.1"
                     /translation="GGVFKIAKQSNKSWSLTHPVDDAITLLTQGGRLTCKFRLSGALT
                     NNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEF
                     GDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDVTKNAAY
                     GVEIESLVLEINAPAA"
     gene            816..1031
                     /locus_tag="ACHY5W_005018"
     CDS             816..1031
                     /locus_tag="ACHY5W_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449714.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1036..>1121
                     /locus_tag="ACHY5W_005019"
     CDS             1036..>1121
                     /locus_tag="ACHY5W_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFS4449715.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVALEIGG"
BASE COUNT          283 a          240 c          327 g          271 t
ORIGIN      
        1 ggtggtgtat ttaagatcgc caagcagagc aataaatcct ggtccctgac gcatccggtg
       61 gatgacgcaa ttaccctgct gacacagggc ggcagactga cctgtaagtt ccgcctgtca
      121 ggcgcactga ccaacaatca gttcgggctg gggatttatc tgtatacgga tgctcccgtt
      181 cctgatggtg tggcgatgac aggtaccggt aatccgttcc tgatgtcgta cttcactcag
      241 accactgacg gcagagtgaa tctgatgcat cacaggaaag ccggaaacac gaagctgggg
      301 gagttcggcg attacggtaa cgactggcag acgctggagc tggtgttcac cgccggcagt
      361 gccacggtta ctccgaaact gaatggagtg gctggcccgg cattccaggt cataaaagac
      421 agtctgacac tggggctgaa tgcgctgacg ctgacggatg ttacaaaaaa tgcagcatat
      481 ggcgttgaga tagaaagtct ggtgctggag ataaatgcac ccgcagcata ataaaaaaag
      541 ccagcgccca ctctgaagga cgctggctaa aacgggtaga tgtacttcac atgatactta
      601 tatctggcag tacattttct gacagatagt gacggatgtt gtcaagatat tgtgtcattt
      661 ataacctgaa tcagggggtg gtcggaatgt tatctggcat ttttagcaga gcctgaatgc
      721 cataatcacg gctcctggcg ttggccgtca gtgggtgaca ctggcggctt ttttgttttt
      781 ctttactttc attttttgtc ggcggtgacg gagacataca tcagatggaa aaaatcacaa
      841 cgggtgtgtc atacaccacg tcagcggtgg ggacgggata ctggttactg cagctgctgg
      901 acaaagtctc tccgtcccag tgggtggcaa tcggtgtgct ggggagtctg ctgtttggcc
      961 tgctgacgta tctgactaac ctgtatttca aaatcagaga ggaccgtcgt aaggcggcgc
     1021 ggggggagta aagcgatgaa gaaaaaatac gaactgggtg ttaaagggat aaataattac
     1081 ccggataaga ttactgttac tgtggcactg gaaattggtg g
//
LOCUS       DAZUGK010000469         1115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZUGK010000469 DAZUGK010000000
VERSION     DAZUGK010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.472"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1115
                     /locus_tag="ACHY5W_005020"
     CDS             <1..>1115
                     /locus_tag="ACHY5W_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449716.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTN"
BASE COUNT          255 a          302 c          360 g          198 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacgcctcga gaggcgaagg ataatctcaa atccacgcag
       61 atgatgagtg tgattgatgc gattggtgag ggaccgatag aaggcccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggcaatcc cgtgatacac
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccgccgga aggttttgag
      241 tcatccggct ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacgt ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg
      361 gagaccacct caaagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagttacag
      421 cgtaacggta actgggtgac agaaaaggac gtcaccatta acggcaagac cacctcacag
      481 ttcctggcct cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 agggagacgg cggacagcac cacggaccag ctgcagaata agacgctgtg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg ccattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg cggtcgcatc
      721 atccaggtgc cgtcaaacta tgacccggaa aaacgcacgt acagtggtat ctgggacggc
      781 agtctgaaac cggcatacag caacaacccg gcctggtgtc tgtgggacat gctgactcac
      841 ccgcgctacg gcatgggaaa acgtctgggg gcggcggatg tggacaagtg ggcgctgtat
      901 gccatcgggc agtactgcga ccagacggtc ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcgtacct gtcacaacag cgtaaggcgt gggacgttct cagtgatttc
     1021 tgctcggcga tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac
     1081 cgcccgtcgg atgtggtgtg gccgtacacc aactg
//
LOCUS       DAZUGK010000470         1090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZUGK010000470 DAZUGK010000000
VERSION     DAZUGK010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.473"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1090
                     /locus_tag="ACHY5W_005021"
                     /pseudo
     CDS             1..1090
                     /locus_tag="ACHY5W_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
BASE COUNT          366 a          259 c          276 g          189 t
ORIGIN      
        1 cctgtggcac tgaacaaata cattactgaa tacctgacta caaagggcgt gtttgaagat
       61 gaaggaagaa atcagagcgc aactgatact ctctcgtcgc cagtaccaga aactgatgca
      121 gtggaaacgg caattccggg caacgaaaaa accgaatgca aagtggaagt cgaaccatct
      181 gtagagcgtg aggggccgtt ctacttcctc ttcaccgaca aggatggcga aaaatacggt
      241 cgcgcaaaca aactttctgg tctggaaaaa gcactagcct tgggagctac ggaaatcaca
      301 aaagaggaat acttcgcacg taaaaacggc acgtactcag gttcacaaca aaatactggt
      361 gcatctgaca cgaccgcaca accagagccg gtaaaagtta ccgctgacga agtaaacaaa
      421 attatgcagc caatatcagc cagcctgacg ccgataagtt gcttgctgcc tctcgcggag
      481 aatttgttgc agggattagc gacccgaatg atccgaaatg ggttaagggg attgaaaccc
      541 gcgattctgt aaaccagaac cagcaagaat cggaacagaa cgaccagaaa gcggaacaaa
      601 acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgagcca gtagcgcaac
      661 aggaaccgga aaaagtctgc accgcctgcg gtcagaccgg cggcggcaac tgccctgatt
      721 gtggcgcggt gatgggcgac gcaacatacc aggaaacatt cgatgaagag tatcaggttg
      781 aagttcagga agatgatccg gaggaaatgg aaggcgctga acatccacac aaggagaaca
      841 ctgacggcaa tcagcatcac gatagcgata atgaaactgg cgagacggca gatcactcaa
      901 ttaaggtgaa cggtcatcaa gaaatcacat ccaccagcag gacgtgtgac catctaatga
      961 tcgaccttga aaccatggga aaaaatcctg atgccccgat catctcaata ggtgcaatat
     1021 ttttcgatcc gcaaaccgga gatatggggc cggaatttag taagactatc gatctggaaa
     1081 ctgctggcgg
//
LOCUS       DAZUGK010000471         1088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZUGK010000471 DAZUGK010000000
VERSION     DAZUGK010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.474"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..252
                     /gene="xisR"
                     /locus_tag="ACHY5W_005022"
     CDS             37..252
                     /gene="xisR"
                     /locus_tag="ACHY5W_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFS4449717.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            254..1036
                     /locus_tag="ACHY5W_005023"
                     /pseudo
     CDS             254..1036
                     /locus_tag="ACHY5W_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
BASE COUNT          361 a          198 c          258 g          271 t
ORIGIN      
        1 ttcagtctaa cattggcgcc tgccagaggt gatgcgatgg cacaagtaat ctttaatgaa
       61 gagtggatgg ttgaatacgg cctgatgctt cgcactggtc tgggggccag acaaattgaa
      121 gcataccgcc agaactgttg ggtggaaggc ttccacttca aacgagtatc tcctttaggg
      181 aagccagaca gtaagcgagg gattatctgg tacaactatc caaagataaa tcagtttatc
      241 aaagactcat gatatgtcta aattaccaac aggtgtcgag attcgaggta aatacattcg
      301 catctggttc atgtttcgag gaaaacgatg tcgggaaaca ttgaaaggct gggaggttac
      361 taacagtaac attaaaaaag ccgggaattt aagagcgttg atagttcatg aaatcaattc
      421 cggtgaattt gagtatttaa gacgtttccc ccagtccagc actggggcaa aaatggtgac
      481 aacgagggtc ataaaaacgt tcggggagct ttgtgatatc tggacaaaaa ttaaagagac
      541 agagttaaca acaaacacaa tgaagaaaac gaaatcacaa ttaaaaacac tcaggataat
      601 aatttgtgag agtaccccaa tatcacatat tcgttatagc gatatcttaa actaccggaa
      661 tgaactgctg catggagaaa cgctttacct ggataatcca agatccaaca aaaaaggaag
      721 aaccgtgcgc acagttgata actatatcgc cctgctctgt tcgttgttac gttttgcgta
      781 tcagtcggga tttatatcaa ccaaaccatt tgaaggagta aaaaaattac agcgaaacag
      841 aataaagcca gatccgttat ctaaaacaga attcaatgca ttaatggaaa gtgaaaaagg
      901 acagagccag aacttgtgga aatttgccgt ttactccggg cttcgtcacg gggaactggc
      961 tgctctggcg tgggaggatg tggatttcga gaagggaatt gtgaatgtca gaagaaacct
     1021 gacgatactt gatatgtgag gtgtactggc aatagcggac actaccattt gttctttttt
     1081 taagcagc
//
LOCUS       DAZUGK010000472         1079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZUGK010000472 DAZUGK010000000
VERSION     DAZUGK010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.475"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1023
                     /gene="cysB"
                     /locus_tag="ACHY5W_005024"
     CDS             49..1023
                     /gene="cysB"
                     /locus_tag="ACHY5W_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFS4449718.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          283 a          254 c          258 g          284 t
ORIGIN      
        1 tgtgacgaaa aaacgatgtt ctgatggcgt ctaagtggat ggtttaacat gaaattacaa
       61 caacttcgct atattgttga ggtggtcaat cataacctga atgtctcatc aacagcggaa
      121 ggactttaca catcacaacc cgggatcagt aaacaagtca gaatgctgga agacgagcta
      181 ggcattcaaa ttttttcccg aagcggcaag cacctgacgc aggtaacgcc agcaggacaa
      241 gaaataattc gtatcgctcg tgaagtcctg tcgaaagtcg atgccataaa atcggttgcc
      301 ggagagcaca cctggccgga taaaggctca ctgtatatcg ccaccacgca tacccaggca
      361 cgctacgcat taccaaacgt catcaaaggc tttattgagc gttatcctcg cgtttctttg
      421 catatgcacc agggctcgcc gacacaaatt gctgatgccg tctctaaagg caatgctgat
      481 ttcgctatcg ccacagaagc gctgcatctg tatgaagatt tagtgatgtt accgtgctac
      541 cactggaatc gggctattgt agtcactccg gatcacccgc tggcaggcaa aaaagccatt
      601 accattgaag aactggcgca atatccgttg gtgacatata ccttcggctt taccggacgt
      661 tcagaactgg atactgcctt taatcgcgca gggttaacgc cgcgtatcgt tttcacggca
      721 acggatgctg acgtcattaa aacttacgtc cggttagggc tgggggtagg ggtcattgcc
      781 agcatggcgg tggatccggt cgccgatccc gaccttgtgc gcgttgatgc tcacgatatc
      841 ttcagccaca gtacaaccaa aattggtttt cgccgtagta cttttttgcg cagttatatg
      901 tatgatttca ttcagcgttt tgcaccacat ttaacgcgtg atgtcgttga tgcggctgtc
      961 gcattgcgct ccaatgaaga aattgaggtc atgtttaaag atataaaact gccggaaaaa
     1021 taatttcttg cgttattttc ggcacctttt atgtagcgaa ggtgtcggaa tatattctc
//
LOCUS       DAZUGK010000473         1076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZUGK010000473 DAZUGK010000000
VERSION     DAZUGK010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.476"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /locus_tag="ACHY5W_005025"
     CDS             <1..117
                     /locus_tag="ACHY5W_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4449719.1"
                     /translation="VYLKDGVVQLAADGAVAAGVAWENAPANSATVAVKINV"
     gene            110..391
                     /locus_tag="ACHY5W_005026"
     CDS             110..391
                     /locus_tag="ACHY5W_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS4449720.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            394..1017
                     /locus_tag="ACHY5W_005027"
     CDS             394..1017
                     /locus_tag="ACHY5W_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449721.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
BASE COUNT          246 a          268 c          353 g          209 t
ORIGIN      
        1 gtttatctga aggacggggt tgtgcagctg gctgcagacg gtgcggtggc agcgggggta
       61 gcctgggaaa atgcccctgc aaacagcgcc actgtggcgg taaaaatcaa tgtctgatct
      121 gtttacgcga atgtgttgcc ggatggacgt ggcgaccgtt cgggtgatgg gcaaacaggc
      181 ggagattaac ggcgtcgtgt acgacgtgat gccggaggaa gagtccgcgg agatgggggc
      241 gctttcgggc agccagttgt cactggtggt gttttcagcc cggtaccgtc cggcccgtca
      301 tgatgttgtt gtgtttgcgg ggcgcacact gacggtgacc cgttatgaca cgtacaacgg
      361 taaaccccgg atttttgtcg aacaggaatg agtatggcaa taaaaggtct ggcgcaggcc
      421 atgaaaaatc tggatgcaat tgatcgccgt gccgttcccc gggcctctgc cacgacactg
      481 aaccgagtgg cgggggccat tattgcgaaa acggcctctt cagttgccag ggagctggcc
      541 gttccccgtc gtcttatccg tgcccgcatc cggttaagtc cggcacgacc ggataaggtt
      601 tacgcaaagg tttacatcaa taccggcaac ctgcccgcca tcaaactggg ggaggcccgc
      661 gttcgacttt cccgcagaaa acggagaaag aaaggacagc gtgcggccct gaaagggggc
      721 ggcagtgtgc tgattgtggg gaaaagacgg atcccggacg cctttatcac ccggctggct
      781 aacggacgct ggcatgtgat gcagcgtatg ccgtgggcat catcatccac cggcgcggac
      841 agcaaaggga ggccgaaacg ccaccgtctg ccgatcgaag tggtgaagat tacgactgcc
      901 ggaccgctgg cagaaacctt tgaacgtgaa cgggaccgga tgtaccggga aaaattaccg
      961 gcgcagatga tgaaagccat gacgcatcag ttacgcctgg tgctgaaaag aaaatgactg
     1021 ggagggtgta tgaaacaccg tgaaatacgg gcggcagttc tgtctgccct gaaaga
//
LOCUS       DAZUGK010000474         1076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZUGK010000474 DAZUGK010000000
VERSION     DAZUGK010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.477"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /locus_tag="ACHY5W_005028"
     CDS             <1..117
                     /locus_tag="ACHY5W_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4449722.1"
                     /translation="VYLKDGVVQLAADGAVAAGVAWENAPANSATVAVKINV"
     gene            110..391
                     /locus_tag="ACHY5W_005029"
     CDS             110..391
                     /locus_tag="ACHY5W_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS4449723.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            394..1017
                     /locus_tag="ACHY5W_005030"
     CDS             394..1017
                     /locus_tag="ACHY5W_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449724.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARGLAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPVQMMKAMTHQLRLVLKRK"
BASE COUNT          241 a          267 c          357 g          211 t
ORIGIN      
        1 gtttatctga aggacggggt tgtgcagttg gctgccgacg gtgcggtggc cgcgggggta
       61 gcctgggaaa atgctcctgc aaacagcgcc actgtggcgg taaaaatcaa tgtctgatct
      121 gtttacgcga atgtgttgcc ggatggacgt ggcgaccgtt cggatgatgg gcaaacaggc
      181 ggagattaac ggcgtcgtgt acgacgtgat gccggaggaa gagtccgcgg agatgggggc
      241 gctttcgggc agccagttgt cactggtggt gttttcagcc cggtaccgtc cggcccgtca
      301 tgatgttgtt gtgtttgcgg ggcgcacact gacggtgacc cgttatgaca cgtacaacgg
      361 taaaccccgg atttttgtcg aacaggaatg agtatggcaa taaaaggtct ggcgcaggcc
      421 atgaaaaatc tggatgcaat tgaccgccgt gccgttcccc gggctgccgc cacgacactt
      481 aaccgtgtgg cggagtccat catcgcgaaa acggcctctt cggttgccag ggggctggcg
      541 gttccgcgcc gtctcatccg tgagcgtatc cgcctgcaac gggccagcgc agacagggtt
      601 tatgcgaagg tcatcatcaa cacaggtaat ctgcccgcca taaaactggg gacggccagc
      661 gtgcggcttt cccgcagaaa gcgacgaaag aaaggcgagc gttcggtcac gaaaggcggt
      721 ggcagtgtgc tgattgtggg gaaaagacgg atcccggatg cctttatcac ccggctggct
      781 aacggacgct ggcatgtgat gcagcgtatg ccgtgggcat catcgtccac cggcgctgac
      841 agcaaaggga ggccgaaacg ccaccgtctg ccgatcgaag tggtgaagat tccgactgcc
      901 ggaccgctgg cagaaacctt tgaacgtgaa cgggaccgga tgtaccggga aaaattaccg
      961 gtgcagatga tgaaagccat gacgcatcag ttacgcctgg tgctgaaaag aaaatgactg
     1021 ggagggtgta tgaaacaccg tgaaatacgg gcggcagttc tgtctgccct gaaaga
//
LOCUS       DAZUGK010000475         1049 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZUGK010000475 DAZUGK010000000
VERSION     DAZUGK010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.478"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1049
                     /gene="escV"
                     /locus_tag="ACHY5W_005031"
     CDS             <1..>1049
                     /gene="escV"
                     /locus_tag="ACHY5W_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFS4449725.1"
                     /translation="LLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHH
                     DAGDIIYSFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGK
                     QMSIDGDMRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLF
                     GGVLIGMWQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEE
                     NLAGDIVQQVSVNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTF
                     GTGNNKDAMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPL
                     PKIIVNPVKNNDSAI"
BASE COUNT          257 a          193 c          248 g          351 t
ORIGIN      
        1 acttttatta atgctctcaa tctatataaa aaatcctctt gaactaactt ctttccccac
       61 aatcctgttg attacgacgt tgatgcgcct gtcgctcagt gttagtacaa ccagacttat
      121 tctgctgcat cacgatgcgg gtgacattat ttactccttc ggtaactttg ttgttggcgg
      181 taatattgtt gttggcctgg ttatttttac tattatcacc attgttcaat ttatggttat
      241 tacaaaaggg gccgagcgtg ttgctgaagt gagtgctcgt ttttcccttg atggtatgcc
      301 aggaaagcaa atgagtattg atggtgatat gcgcgcaggc gttattgacc cgcttgaggc
      361 aaaagttctt cgctcccgag tgcaaaagga aagccagttt tatggttcaa tggacggggc
      421 gatgaagttt gtaaaagggg acgctattgc tggtatcatt attgttttag ttaacctttt
      481 tggtggcgtg ctcattggta tgtggcaatt tgacatgcca tttagtgcgg cacttagcct
      541 gttttctgta ttgtctgtcg gtgatgcttt ggttgcccag atccctgcgc ttattatttc
      601 tgtcaccgcg ggcgtggttg ttactcgtgt gcccggtgaa agcgaaaaag aagaaaacct
      661 tgcaggtgat attgttcaac aggtttctgt taatagtcgc ccttttttaa tcagtgccgc
      721 actaatgctc gtaatggcga ttattccggg ctttcctaca ttggtcttct tatttctggc
      781 ggtttgcctg ttggggatag cctggaaatt acagaagaaa agaacatttg gaactggcaa
      841 taataaggat gctatgggag ctgatttgtc taatagccaa aacatctcac cgggcgctga
      901 gccattgatt ttaaacttaa gtagtaatat ttatagttca gacattacac agcaaattga
      961 ggtcatgcgt tggaatttct ttgaggaaag tggaattcca ttgcctaaga ttattgttaa
     1021 tccggttaaa aataatgata gcgcaatag
//
LOCUS       DAZUGK010000476         1048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZUGK010000476 DAZUGK010000000
VERSION     DAZUGK010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.479"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(395..967)
                     /gene="hdeD"
                     /locus_tag="ACHY5W_005032"
     CDS             complement(395..967)
                     /gene="hdeD"
                     /locus_tag="ACHY5W_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFS4449726.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGVLLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          363 a          240 c          205 g          240 t
ORIGIN      
        1 aataacgcat tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca
       61 atgtaatcaa cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc
      121 atcaacgctt acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg
      181 actacggaaa atatcagcca agataatgtt tgaaatggct aatttgccat ggagtgaaaa
      241 aaattagatg aaattcagta ggttgaaata atcactagca ggtaattatt tcaatgatag
      301 tgcgcaattg atctacaacg ctgcgtagcg gagagagtat taatcggatc atagtcacat
      361 caagtgacta tgatcctggt gacaaccggg ataattattg ctgcttaacg aacaaactgg
      421 cgaagctgaa caggctggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca
      481 gcgttacaga caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca
      541 caccaataac gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca
      601 ggcgaataac ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc
      661 ccagctccgg tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga
      721 aaccggataa taccggccag aaattatgac tgcggttgct gaataacccg acaataagcg
      781 caatacccga gcagattaat aatacaccca ctactgtgct taaaatatcg ccagagacga
      841 acgggaaact gatacacagc aacccgacga taaacagcag cacggcaata aactggattg
      901 ctctgcgatg ttttttaagc atctccagat caaacttcaa aattgttgcc ttatctatat
      961 ataacataga accaccctat aaaattaaga agaaaatccc ctgctatcaa tctatgccaa
     1021 aaacgcgtct aagaatgcag tcgattta
//
LOCUS       DAZUGK010000477         1033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZUGK010000477 DAZUGK010000000
VERSION     DAZUGK010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.480"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(396..631)
                     /locus_tag="ACHY5W_005033"
                     /pseudo
     CDS             complement(396..631)
                     /locus_tag="ACHY5W_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(802..>1033)
                     /locus_tag="ACHY5W_005034"
     CDS             complement(802..>1033)
                     /locus_tag="ACHY5W_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFS4449727.1"
                     /translation="PITLAIPQRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSR
                     EPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          313 a          241 c          187 g          292 t
ORIGIN      
        1 actaaaaatc gcccaagagc attctgtttc tataatgtat tccaagagat taaaaaataa
       61 tatcatacgt tattcggcag agttcgtatg tactacggcg tcccaaccat gtcccaaaac
      121 aaaaatttct gccctataca cgttccaaaa aattcaacag caaagagagt ctgctgacga
      181 gttgttgatt cactgattaa tacgtaaaat tgataactgc tggaaatcat acaatactcg
      241 tactttcgaa agttcgctaa ccaggtgcta cacgcgaggt gttgcagttt tctttacgat
      301 ttatcattca atcctaatcc atgcatgaca tgtggtgcct gtggggtgat cctacctacg
      361 taatgtggac acagccctaa tcaaggttct gtttttcaaa acagttccgt gctgagacag
      421 ccacaagtac tgtgtcggca ccaccgattg taatcacact agatctaatt aaacacagcc
      481 gtctgcatta tttcccgact cataaaatat tagccgtaaa tgcattcgac ttttagtgag
      541 tggaagaaac tttccgccct agcattatcg taacagcgcc cctttgcact catcctgtca
      601 tgcaggtatg ccgcttcaga agcgcctgat aatgcgttga aatcaggacg gtggctactt
      661 gctattcagt tcacgataca aaatgggcaa tatcccttaa ggaccagcga atagcaatga
      721 gtcactttat tatcaagttt gatgaccgct ttgatgctaa cttttaataa gagtctcttc
      781 acacaaaatc gtgtaccgag tctacactga ataacaatca ctcctcaccg taaagtgacc
      841 gatactgcaa tcaaactcac actgctctct ggccacaagc attgataatg ttattggctc
      901 tctgcttagt gggtgatgca tcccatcatt aattatacga gaaagagcca tttcatcata
      961 taaactacat actctggaac cttcagcatt tcttaaaaaa acacctcgtt gaggaattgc
     1021 cagcgtaatc ggg
//
LOCUS       DAZUGK010000478         1027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZUGK010000478 DAZUGK010000000
VERSION     DAZUGK010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.481"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..393
                     /locus_tag="ACHY5W_005035"
     CDS             <1..393
                     /locus_tag="ACHY5W_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS4449728.1"
                     /translation="IWTICRGTTRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVER
                     NIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDLKGACEAIRWWIKDGGRD
                     CRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            549..731
                     /locus_tag="ACHY5W_005036"
     CDS             549..731
                     /locus_tag="ACHY5W_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449729.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            744..875
                     /locus_tag="ACHY5W_005037"
     CDS             744..875
                     /locus_tag="ACHY5W_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449730.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            883..>1027
                     /locus_tag="ACHY5W_005038"
     CDS             883..>1027
                     /locus_tag="ACHY5W_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS4449731.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLAN"
BASE COUNT          256 a          212 c          299 g          260 t
ORIGIN      
        1 atctggacca tctgccgcgg taccacccga gtggatggta aacctgtcgt cccgggcatg
       61 aagttgtcga aggaaaaatg cgaccaggtt aacgccattg aacgtgataa ggcgctggag
      121 tgggtggagc gcaatattaa agtaccgctg accgaacccc agaaggcggg tatcgcgtca
      181 ttttgtcctt ataacattgg ccccggtaag tgtttcccgt ctacgtttta cagacggatt
      241 aatgcaggtg atctaaaagg tgcctgcgaa gcgattcgct ggtggattaa ggacggtggc
      301 agagactgcc gtattcgctc aaacaactgc tacggtcagg tttcacggcg tgaccaggag
      361 agcgcgctgg cgtgctgggg aattgacaga taagcagaat attttgctga aaaatgcggt
      421 ttgctcacac tggcggataa cacgaaatcc tgcgaactgg caaaaactaa gtgaataaaa
      481 gtaaaacccc gtttgttggc cgcaaacggg gttttgtgtt tctgaccttg agtaaggcaa
      541 gggagaacat gggaaagtat aaacgaattc tgttgagatt gactatgaaa aacggccttg
      601 aactgaaagc gcctgtaact gatgacgtca gcagagcgct ggcttttgct attaagtggg
      661 tggcggtcgg tattgctgtg tctccgatgc tgtatgggct ggcaaaactg gtcattgcgt
      721 tgaaatcgtg aagaggatta agcatgtcag acaagctcat aacgctggcg aagatcctct
      781 gtgtaatcgt cggcatttca ttttcactaa tgctggttgc tatttgcttt tccactgcct
      841 ggcgcgtctt aactttgtca tggctggtgg ggtgaggggg atatgaaccg tgttctgtgt
      901 gtggtgatta ttgtcctgct ggtagcctgt ggtgtgctta gtctggggct gaatcattac
      961 cgcgataacg ccattaccta caaagcgcaa cgcgataaaa aagccagtga gctgaagctg
     1021 gcgaacg
//
LOCUS       DAZUGK010000479         1019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZUGK010000479 DAZUGK010000000
VERSION     DAZUGK010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.482"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..124
                     /locus_tag="ACHY5W_005039"
     CDS             <1..124
                     /locus_tag="ACHY5W_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFS4449732.1"
                     /translation="ALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKAPVLH"
     gene            139..>1019
                     /locus_tag="ACHY5W_005040"
     CDS             139..>1019
                     /locus_tag="ACHY5W_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFS4449733.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPTADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDA"
BASE COUNT          202 a          310 c          321 g          186 t
ORIGIN      
        1 tgccctgctg gaagatttaa tggaatccgg ccccctgaag gccgcgccgt cgctgtcctt
       61 tctcgggctg gttgtgatgg atgaactctg tgcccgtcac ataaaagcgc cggtactgca
      121 ctgaaggaga acaacaccat gaaaatgaac gtaaccgcca ccgtcagcca tgcgctcggc
      181 cactggccgc gtattctccc ggcgctgggg attcaggtgc tgaaaaaccg tcatcagccc
      241 tgtccggtct gtggcgggag tgaccgcttc cgttttgatg acagggaggg gcgcggcacc
      301 tggtactgca atcagtgtgg tgccggtgac ggcctgaaac tggttgaaaa ggtgtttggt
      361 gtttccccgt ccgacgcggc cgcaaaggtg gctgccgtga ccggcagcct gcccacggct
      421 gacccggcag tgacggccgc cgccggcgct gaaacagacg ctgcccggaa gaacgccgcc
      481 gcactggcac aaaccctgat ggcaaaaacc cgtcccggaa ccggtaacgc ctacctgacc
      541 cgcaagggct ttcccggccg ggaatgccgg atgctgaccg gcacacacag agccggtggc
      601 gtgagctggc gcgccggtga ccttgtggtg ccactgtatg acgacagcgg cgaactggtt
      661 aaccttcagt taatcagtgc tgacggccgt aagcgcaccc tgaaaggcgg acaggtcagg
      721 ggcacctgtc acacccttga aggacagaat caggccggaa aacgtctgtg gatagcggag
      781 ggatacgcga ccgcacttac cgtacatcac ctgaccggtg aaacggtgat ggtggcgctt
      841 tcttccgtga acctcctttc tctggccagc cttgcccggc agaagcatcc ggcctgtcag
      901 attgtccttg ccgcagaccg tgacctcagc ggtgacggcc agaaaaaagc cgccgcagcc
      961 gcagatgcgt gtgagggcgt tgttgccctg ccgccggtct tcggtgactg gaatgatgc
//
LOCUS       DAZUGK010000480         1004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZUGK010000480 DAZUGK010000000
VERSION     DAZUGK010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.483"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..>1004
                     /gene="cif"
                     /locus_tag="ACHY5W_005041"
     CDS             212..>1004
                     /gene="cif"
                     /locus_tag="ACHY5W_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFS4449734.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFE"
BASE COUNT          299 a          193 c          232 g          280 t
ORIGIN      
        1 gtgtagtgga tttgttgcat ggatggagtt ggtaaaaagt ggtgtggctg gcaatccagg
       61 ccgcgtcaca gaaatggaca atgccgctga gagactggcg aatggcatac aggcaccaga
      121 aaaatttcag acatgacata atgcccatgc cagaagttct ttttgcacct cgcctgtatt
      181 tatagataaa catagagtga atgaatgaga tatgaaagac attacccttc cccccccgac
      241 gtccgcgtcc tgtctgacag gggccatatc tgtaaatact gaagctgtat tatctcccat
      301 gcaacacact tcagccttac atgtaagaga ttttgcttcc ctgtgctcac agaacctcaa
      361 agctaatgta ttgctaaata gtgatgacca cgaagtacct atacatcaga aaaatcctgc
      421 tgcaataatg caaaatatcg actctaacat caaacagatg gcaacagact gggggatgtc
      481 gattgaggag gttgaggtta ttatagggcg agagaaaggc attgtggaac cctcctgcgg
      541 agttaccgct aatgctatta tgaaactatt tctggacaag gatggcttca gttactgctt
      601 tgaaaatgaa cagacactat cgctcgagca gcttcaggag cgcctgtcct gtatgcctga
      661 atgtaagagc tttgtattac gtgttaatga tggtgcgctt ggtcatgctt acattgtcga
      721 tattcccaaa ggagaaaact cttgtcgtcc tgcattcttg tatcagtcag atttaggaga
      781 gggcgtcacc agaaagttaa gatttgagga ctggatgacg cataaagcat tgactccgat
      841 tttgctggat gatatttgta attacttctc ctgcatgtct caaaataaga cagatttgga
      901 gcagattgca acgttatttg atattgatgg aaatgttaaa atgttacgaa aggaaaatat
      961 tcaatatcaa aagcatgaca attttagttt ccagttgttt gagt
//
LOCUS       DAZUGK010000481          998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZUGK010000481 DAZUGK010000000
VERSION     DAZUGK010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.484"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>998
                     /locus_tag="ACHY5W_005042"
     CDS             <1..>998
                     /locus_tag="ACHY5W_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFS4449735.1"
                     /translation="QATGAEVTSESVFNILNSTGAATDKSYLSLNPDKYPNYRLLIHS
                     AKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGTFILASTFEDDKTAAETHYDA
                     VWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPDQIKRMQDIISNPKRLDGIPG
                     QMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGLYLDLLIQAINTGTINAEDWQ
                     KGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSNLLSKKD
                     SVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEKDKHY"
BASE COUNT          322 a          201 c          225 g          250 t
ORIGIN      
        1 ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg
       61 ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtatccta attatcgtct
      121 tctgattcac tcagccaaat tacaaaatga aataaagagc cactatacca aagacgaaat
      181 tcaaggattg ctaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg
      241 gggaacgttt attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta
      301 tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat cagaccaggg
      361 aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgcccga
      421 ccagattaag cggatgcagg acatcattag caacccaaaa cgactggatg gtattcccgg
      481 gcaaatgaat gctgtgcata tccgatttga cagcaactcc cccgtgatgg cagatgtgca
      541 ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag gtctttatct
      601 tgatctcctt atacaagcaa tcaatactgg tactattaac gctgaagatt ggcaaaaagg
      661 tgaccgacta aaatcggttg cgctgctgat tgcttatctg gataaagcca atttctatgt
      721 tatggaagat tccggagcgt gggaggaaga tgcccgatta aacacatctt ccgttgcact
      781 ggtgacctct ggactggaac gtttgtcaaa cctactctcg aagaaagact ctgtttttgt
      841 ctctgattta ctacgcgaag ctaaagtcaa tgaactggat gaaactcttt caacaacgag
      901 gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg atttaggggg
      961 agaatcccca ggttatctgg aaaaagataa acattaca
//
LOCUS       DAZUGK010000482          981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZUGK010000482 DAZUGK010000000
VERSION     DAZUGK010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.485"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..>981
                     /gene="tdcA"
                     /locus_tag="ACHY5W_005043"
     CDS             101..>981
                     /gene="tdcA"
                     /locus_tag="ACHY5W_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFS4449736.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSTEMKL
                     QDLHVEALFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELA"
BASE COUNT          272 a          187 c          224 g          298 t
ORIGIN      
        1 ggaaagtatt gaaagttaat ttgtgagtgg tcgcacatat cctgttcatt tcattttgat
       61 acacttcatg ccgtcaatga ggtaattaac gtaggtcgtt atgagcacta ttcttcttcc
      121 gaaaacgcag cacctggtag tctttcagga agtcattaga agtggctcta tcggctcggc
      181 tgcaaaagaa ttagggttaa ctcaaccagc cgtcagtaaa atcattaacg atattgaaga
      241 ttattttggt gtggaattag tggtgcggaa aaataccggt gtaacattaa cacctgccgg
      301 tcaattgtta ctctcccgtt ccgaatccat tacccgtgaa atgaaaaata tggttaatga
      361 gataagcggt atgtcttctg aggcggtggt ggaagtctca tttggttttc cttcattgat
      421 tggttttact tttatgtcag ggatgatcaa taagttcaaa gaggtgttcc caaaagcgca
      481 ggtttctatg tatgaagcgc aactgtcttc gttcttaccg gcaatccgcg acggtcgact
      541 tgattttgcg attggtacgt taagcacaga aatgaagctt caggatttac atgttgaggc
      601 gctgttcgag tccgagtttg tgctggtagc cagtaagtcc cgaacatgca ccggcaccac
      661 cacgctggag tcgttgaaga acgaacagtg ggtgttgcca caaaccaata tggggtacta
      721 cagcgaactg cttactacgt tacaaagaaa tggcatcagt attgaaaaca tcgttaaaac
      781 cgactcagtc gtgacaattt ataatcttgt tctcaatgct gatttcttaa ctgtaattcc
      841 ttgtgatatg acgtcacctt ttggttctaa tcaatttatt actattccgg ttgaagaaac
      901 attacctgtg gcacaatatg ccgcggtatg gtcgaaaaat tatcgtatta aaaaagcagc
      961 atcggttttg gtggaattag c
//
LOCUS       DAZUGK010000483          976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZUGK010000483 DAZUGK010000000
VERSION     DAZUGK010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.486"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..905
                     /gene="hlyE"
                     /locus_tag="ACHY5W_005044"
     CDS             3..905
                     /gene="hlyE"
                     /locus_tag="ACHY5W_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFS4449737.1"
                     /translation="MVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          328 a          186 c          209 g          253 t
ORIGIN      
        1 aaatcgttgc agataaaacg gtagaggtag ttaaaaacgc aatcgaaacc gcagatggag
       61 cattggatct ttataataaa tatctcgatc aggtcatccc ctggcagacc tttgatgaaa
      121 ccataaaaga gttaagtcgc tttaaacagg agtattcaca ggcagcctcc gttttagttg
      181 gcgatattaa aaccttactt atggatagcc aggataagta ttttgaagca acccaaacag
      241 tgtatgaatg gtgtggtgtt gcgacgcaat tgctcgcagc gtatattttg ctatttgatg
      301 agtacaatga gaagaaagca tccgcccaga aagacattct cattaaggta ctggatgacg
      361 gcatcacgaa gctgaatgaa gcgcaaaaat ccctgctggt aagctcacaa agtttcaaca
      421 acgcttccgg gaaactgctg gcgttagata gccagttaac caatgatttt tcagaaaaaa
      481 gcagctattt ccagtcacag gtagataaaa tcaggaagga agcgtatgcc ggtgccgcag
      541 ccggtgtcgt cgccggtcca tttggtttaa tcatttccta ttctattgct gcgggcgtag
      601 ttgaagggaa actgattcca gaattgaaga acaagttaaa atctgtgcag agtttcttta
      661 ccaccctgtc taacacggtt aaacaagcga ataaagatat cgatgccgcc aaattgaaat
      721 taaccaccga aatagccgcc atcggggaga taaaaacgga aactgaaaca accagattct
      781 atgttgatta tgatgattta atgctttctt tgctaaaaga agcggcaaaa aaaatgatta
      841 acacctgtaa tgagtatcag aaaagacacg gtaaaaagac actctttgag gtacctgaag
      901 tctgatcagc gattattctc tccatgtact caaggtataa agtttatcac atttaaaaat
      961 acagacgttt tttaaa
//
LOCUS       DAZUGK010000484          971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZUGK010000484 DAZUGK010000000
VERSION     DAZUGK010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.487"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(662..>971)
                     /locus_tag="ACHY5W_005045"
     CDS             complement(662..>971)
                     /locus_tag="ACHY5W_005045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449738.1"
                     /translation="ILSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQN
                     GYFMERSNFLVAIIMLILRSLAIIPGWFVSLGHEIVTFLFIMEIWGKAGNDFFIYIRW
                     "
BASE COUNT          294 a          173 c          188 g          316 t
ORIGIN      
        1 ttctgatgtt taattgattt gaatgttcgt aagctatatc acttactcaa ttcattttac
       61 ccagagtcat aaaatagagt gtctattgat ctcattttca tattcctttc agactgaagt
      121 gcgttttcat cacccgatga gaaagaactg aagtgatgtt tgccatagat tgcgagatgc
      181 cctgtctcat tgactatctt ggtgcacctt tatacctgtt ataccagatc aaaaatcacg
      241 caatccatac aacaaaacca gatttgcaat tcgtgtcaca aaatatgtcg atctttttct
      301 aagaggaaga tgccatgtga agccagacga acacttgcag tggtcttcaa aaactaaaga
      361 tcttctttta actatttgtt ttataaaaat ttatttagag tctaaacaag ggaagctttg
      421 caagctaact cagtgagctt ggtgaaaatc agtgtttacc cgccatcagg ctgagcataa
      481 ttctcatcat gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt ctgaagtcga
      541 tctggagagg cttgttgatg ttggtgtttt caggatgatg tttcacttag tttgtttgcc
      601 gtatcgcccg gcgaatggct gtgattgagg aaggtttaag tcgtagtgac caaagccata
      661 tttaccaacg aatgtagatg aaaaaatcgt ttcctgcttt cccccatatc tccatgataa
      721 aaaggaatgt aacaatctca tggcctaaac tgacgaacca gccaggaata atcgctaggg
      781 acctaagaat tagcatgata atagccacta agaaattact gcgttccatg aaatagccat
      841 tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt gaacgaatat
      901 taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc acatgatata
      961 tagataagat t
//
LOCUS       DAZUGK010000485          964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZUGK010000485 DAZUGK010000000
VERSION     DAZUGK010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.488"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>964)
                     /locus_tag="ACHY5W_005046"
     CDS             complement(<1..>964)
                     /locus_tag="ACHY5W_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449739.1"
                     /translation="ETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGV
                     WGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGV
                     TPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTAEDGSERLVSTARTT
                     ETTYRFRQLALGNYRLTVRAANARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITAT
                     PHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIR
                     SVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQI"
BASE COUNT          184 a          308 c          268 g          204 t
ORIGIN      
        1 caatctgcgt ccacagctcc tgagccagat gggttttccc tatctcgcct ttgaaaaaat
       61 ccagatagcc ggatgcatca tcactcggct gaccaacagc ctccacgaat gccgatttgc
      121 caacggtgtt cacactgcgg atataaaagt aataatcatg gcccggtttg atattgatac
      181 tggcggctat ccagtacagc cccgtgccaa gatagcgggc tgtggtttca acctgcctga
      241 tatcggtaat ccgcgtttcc gagaaccaga actcaaactg taccgtcgga tcataaaccg
      301 caagatgcgg cgtggcggtt atctgaaaat agcccggtgt cagctcaatc cgagacggcg
      361 ctgccggtgc ggcaatccgg aacgataccg acgccggatc gccctgctgc ccccgggcat
      421 ttgccgcccg gactgtcagc ctgtagtttc ccagcgccag ctgcctgaag cggtatgtgg
      481 tttccgtcgt ccgggctgtg ctgaccagcc gctcactgcc gtcttctgct gtcacggtca
      541 gacgaagcag gaagctcacg cccttcacca ccttcggcgt atcccagcgc gccagcacct
      601 gatattcccc gctgtctgcg gtgacttcgg cagtcaggtg ctgcaccgcg ggcggcgtga
      661 caccattcac cgtgccgctc tggtcgccgt caaagtgcgc cccgttatcc acgatggctt
      721 ctttttccgg cacgtgctgc accgccgtga tggcaaaggt gccgtccgtg ttttcccgga
      781 tggagacaca gcggaacagg cgacgacgca gtgacggcag ggagagtccc catacaccgt
      841 atgtctccac accatcaggc agggtgctga cctgtatccg gtccggcgcg gggtgtgcag
      901 tgatggccac gctcaccggc ttaccgctgc cgttaatcag gttcaccgtg gcggcacctg
      961 tctc
//
LOCUS       DAZUGK010000486          962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZUGK010000486 DAZUGK010000000
VERSION     DAZUGK010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.489"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHY5W_005047"
     CDS             complement(<1..143)
                     /locus_tag="ACHY5W_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS4449740.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIA"
     gene            complement(197..>962)
                     /locus_tag="ACHY5W_005048"
     CDS             complement(197..>962)
                     /locus_tag="ACHY5W_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFS4449741.1"
                     /translation="DALALTNSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYT
                     LVLRCRDAVNKGSVPHKFRKWVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLV
                     GKKGLRNDDAYNMVHQRFGIDSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNEL
                     SAKEANSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVL
                     INHTRDVDINEPDGPTNLSAWMRLKNKELPPSPHHY"
BASE COUNT          236 a          237 c          209 g          280 t
ORIGIN      
        1 gcaatacgaa aatatgcctc aggctctgca gagatattca cacaagggat ataccgctcc
       61 ccttccggcg ttctcaccac gaagccgcac gactccgctg gcgcacatcg ccgggcgtgc
      121 gccaatatat tgctatagag catgagaact cctgataaaa acccagccga agctgggtca
      181 tttcgttggc aatctgttag tagtgatgcg gtgaaggagg taattcttta ttcttaagtc
      241 tcatccatgc ggaaagattc gttggtccgt ctggctcatt gatatcaaca tctcgtgtgt
      301 gattaattaa aacgtctctc gccattccaa taacatacga gaactcatga ccgtagtcgt
      361 agcatctgcc ggaatagttc gattgaattt gttttagcgc cggatacagt tcgcggaata
      421 atgcctgtga gcggttggca taatcccata gccatacaag gctgtttgct tcttttgcgg
      481 aaagctcgtt ggtgctcttc tcttgtttgc caatgaattc accttcaagc actaccctgt
      541 ggatgtactc tacggcaagc gggatttgtt caattgaaag ctcatcaatg ctgtcaatac
      601 caaaacgctg atgaaccata ttgtatgcat cgtcattgcg aagtcctttc tttcctacca
      661 gcatgtttac tgcatcgcgt agcggtgttc tttcctcaac agtggttttc tttcctttta
      721 catactcgcc atgtttgcga attgaaggca gaacttctgc tgttacccac ttgcggaatt
      781 tgtgcgggac tgaaccttta ttgactgcat cgcggcagcg cagaaccaat gtatacatac
      841 ctgattcgct cacaatgctt agattctgct caccaccaag ggtgtaactt aaagttactc
      901 ccttttcatc gtcatcaagt gcagtaagcg ccttgcgtga gttagtcaaa gctaaagcat
      961 ca
//
LOCUS       DAZUGK010000487          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZUGK010000487 DAZUGK010000000
VERSION     DAZUGK010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.490"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>943
                     /locus_tag="ACHY5W_005049"
     CDS             <1..>943
                     /locus_tag="ACHY5W_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449742.1"
                     /translation="SRALIMAYEAVKPTRTHKARRENRSADQLSKYGAVSLREQARFL
                     DINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEMAAELAADIRRLWAEWSVSPDV
                     TGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLERTAGVPFWLEAMEPDFVPMRTD
                     ESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKEIAAGKMIHLKFTRRLHQTRGS
                     SMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKGDGQDYEDPGIKETEREVHITP
                     GIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVAA"
BASE COUNT          218 a          207 c          306 g          212 t
ORIGIN      
        1 gttcaagggc gttaatcatg gcctatgagg cggtgaaacc gacccggaca cataaagccc
       61 ggcgggaaaa tcgctctgct gatcagctca gtaaatacgg tgcggtttcc ctgcgggagc
      121 aggcccgttt tctggatatc aatcatgacc tggtgattgg tgtgtttgac aagctggaag
      181 agcgggtgat tggtgccagg ggaattattg tggagcctca gccattacga aaaaacgggg
      241 aaatggcggc tgagctggct gcggatatcc gccgtttgtg ggctgaatgg tccgtgagtc
      301 cggatgtgac agggcagtat acccgtcctg tgcttgaacg tttactgctg cggacctggc
      361 tgcgggatgg tgaagtgttt gcgcagatgg tcagtggtgc gggaaacggt ctggaacgga
      421 cggcgggagt gccattctgg cttgaggcga tggagccgga ttttgttccc atgcgcactg
      481 atgaatccgc cggactgaat cagggggttt ttcttgatga gtggggaaga ccgaaaaaat
      541 atctggttta taaaaattat ccggtcagcg gccggcagag tgatacgaaa gaaatcgctg
      601 ccggaaaaat gatccacctg aagttcactc gtcgtctgca tcagacgcga ggctcatcca
      661 tgttatcggg ggtgctgatg cggatcagtg cccttaagga gtatgaggat gcggaactga
      721 cagcggcgcg tattgctgcg gcgctgggac tgtatatccg taaaggtgac ggacaggact
      781 atgaagatcc ggggatcaaa gagaccgagc gggaagtcca tatcaccccg ggtattattt
      841 atgacgattt gcgcaagggc gaggatatcg gcatggtcaa atctgaccgt cccaatccca
      901 accttgaaac tttccgcaac ggccagttgc gtgcagtggc agc
//
LOCUS       DAZUGK010000488          933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZUGK010000488 DAZUGK010000000
VERSION     DAZUGK010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.491"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(151..822)
                     /gene="queE"
                     /locus_tag="ACHY5W_005050"
     CDS             complement(151..822)
                     /gene="queE"
                     /locus_tag="ACHY5W_005050"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFS4449743.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          250 a          228 c          209 g          246 t
ORIGIN      
        1 cagtgaaaaa ttaacgccac agaatttgta gagaatgata atgattagtt caggatgggc
       61 aaaatggagc tttttcagag acgacaaaga atataaatga gtagaagtct ttacaggttc
      121 cacccattta taacgcttat aaatgtttaa tcaggcaata tttaaatatt tatgggtttg
      181 catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg tggcatcatc
      241 cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgtcgccaa
      301 aagttcatcc agtgcttcaa tatcacgtac gcgtcccact gggtgcttga tttcgttggc
      361 tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac
      421 ggtaacccag gtattcggtg tacagcgtac ctcatgagta ccactggtct cgatctggca
      481 gctaaaaccg ttcttttcga gcagatcagt cagtggtagc aaatcatgaa tgcaaggctc
      541 accacccgta atcaccacat gccgcgcggt gtatccctgg cgactaatga cagccaacaa
      601 gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa
      661 aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca
      721 gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc
      781 accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg
      841 gataattgtt aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta
      901 caggcaggtt taaggcaaaa agtgcattag atc
//
LOCUS       DAZUGK010000489          927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZUGK010000489 DAZUGK010000000
VERSION     DAZUGK010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.492"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..849
                     /locus_tag="ACHY5W_005051"
     CDS             <1..849
                     /locus_tag="ACHY5W_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4449744.1"
                     /translation="KIPIMLSSSIQAELDNAYGVSKALAERYIEKYATTSGSPYYIFR
                     YPNVFGKWCKPNYNSFVATFCYNIANDLAITINDASAPVNLVYIDDVCAKAIELLSGA
                     TESGYKAVAPIYSTTVGEVAELIYRFKNSRSTLITEAVGTGFTRALYSTWLTYLPAEK
                     FAYKVPFYGDARGVFCEMLKTPSAGQFSFFTAHPGITRGGHYHHSKNEKFLVIRGQAC
                     FKFEHVITGERYELNVSSDEFKIVETVPGWTHDITNIGTEELIVMLWANEIFNRDEPD
                     TIARPL"
BASE COUNT          261 a          161 c          204 g          301 t
ORIGIN      
        1 aagataccaa ttatgctaag ttcttctata caagcagaac ttgacaatgc ttatggggta
       61 agcaaagctt tagctgaacg ttatattgaa aaatatgcta ctactagtgg ttctccatat
      121 tatattttca gatatccaaa cgtttttggc aaatggtgca aaccaaatta taattctttt
      181 gttgcgactt tttgctataa tattgcgaat gatcttgcca taactatcaa tgatgcatct
      241 gcgccagtta atttggttta tattgatgat gtttgtgcta aagcgataga gcttctctcg
      301 ggggcaactg aaagcggata taaagctgtt gcaccaattt attcaactac tgttggtgaa
      361 gttgcagaat taatttatag attcaagaat agccgttcca ccctgatcac agaggctgtc
      421 gggacgggat ttacccgtgc attgtattct acatggctga cttatttacc agcagagaag
      481 tttgcgtaca aggtaccttt ttatggggat gcccgcggag tcttttgtga gatgttgaaa
      541 acgccttcag cggggcagtt ttcatttttt actgctcacc ctggtattac gcgtggcgga
      601 cattaccatc acagtaaaaa tgagaagttt ttggtcattc gaggtcaggc atgctttaaa
      661 tttgaacatg tgattaccgg tgagcgatat gaactgaatg tttcatcgga tgagttcaag
      721 attgttgaaa cagttcctgg ttggacacat gacattacaa atattggaac tgaggaatta
      781 atagtcatgc tctgggcaaa tgaaattttc aaccgtgatg agcccgatac tattgcgaga
      841 cctctataat gaaaaaatta aaagttatgt ctgttgttgg aacccgtcct gagattatcc
      901 gtttgtcgag ggttcttgct aagtttg
//
LOCUS       DAZUGK010000490          927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZUGK010000490 DAZUGK010000000
VERSION     DAZUGK010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.493"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..307
                     /locus_tag="ACHY5W_005052"
     CDS             98..307
                     /locus_tag="ACHY5W_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449745.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(308..>927)
                     /locus_tag="ACHY5W_005053"
     CDS             complement(308..>927)
                     /locus_tag="ACHY5W_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449746.1"
                     /translation="PSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRDAFTGLYF
                     CASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAGNMFVY
                     DNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAEKRWNVAT
                     WLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          252 a          206 c          181 g          288 t
ORIGIN      
        1 actgtcagta ccggattctt atccgtgtcc ggcgcacgac cacacgtgac agcgtgttgg
       61 tctccatttt taacccagaa cctcaatgga ggataaaatg ccaaacaaaa aaagaaatcc
      121 gcttattgaa aaacagattg aatgcctggt aaatcaactc aggcaatcag ggttattaaa
      181 aactcattca gagttgaggc tcacagaatc agcattcgac gataaattaa ataatgtcct
      241 ttataatggc attattgatt ttaatcgttc tgttggtcgc cgcggccctg ctggtgtttc
      301 cttataatta ccagtcaatc cagagtggac cgtgttcagc gtaaatataa ctgtacacat
      361 ccagattata tttgtggtct gttaagaaca ggccgcaaat acatgccgaa gcttccagtg
      421 cagcggctct gttactgaat aaccatgtag caacattcca gcgtttttct gcatcccagt
      481 ctttctcaag gcctgatacc atgaagaaac cgttagtgtt gccatcaaat aattctgttt
      541 ccaaattttt aagcaatgcc tgatggactc ttgccaggta ttccgccgga atttcgccac
      601 gaattctgat gagattgtca taaacaaaca tgttccccgc atatggcgat ttttctttct
      661 tgttttttaa accagcatca tgagcaaact gatcaatttc ttcttccgtt ggtttcgtat
      721 tgatgttttg cgctgtcgtt tctgcaattt tatttgccac actctctgag tcgtgtttat
      781 ttatagacgc acagaaatac aatccggtaa acgcatcgcg cacattacga gccatattat
      841 cagtgtcttt tttcgttacc gattccaatt caagttcgtt cagacgatga cgaagtgtgt
      901 gtgctgcaat ctcctggatt gaaggag
//
LOCUS       DAZUGK010000491          925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZUGK010000491 DAZUGK010000000
VERSION     DAZUGK010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.494"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..810
                     /locus_tag="ACHY5W_005054"
     CDS             10..810
                     /locus_tag="ACHY5W_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFS4449747.1"
                     /translation="MLKEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGASRNVM
                     HMSRRVTTDKDTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEAD
                     SAYALKADSGAVQQVMTARPEQSHQLQQPEADSAIQREADRVVPENTGLSVGRVDYPD
                     VFEQVWREYPLRAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTE
                     FVQRATTFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            817..>925
                     /locus_tag="ACHY5W_005055"
     CDS             817..>925
                     /locus_tag="ACHY5W_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4449748.1"
                     /translation="MKNIAAAGVLERIRRLAPPASVPPYRTVEEWREWQL"
BASE COUNT          253 a          226 c          290 g          156 t
ORIGIN      
        1 aaacgccagg tgcttaaaga ggacaacggc aatatttcgc aaaccgcaaa atctcctgca
       61 gagcgcaaga gggcgcagcg agagagggaa agaaagcggg aacaaaatgg cgattgtcac
      121 ggcgcgtcac gaaatgtcat gcacatgtca cgacgagtca cgacagataa agatacagat
      181 aaagatacag ataaagatac agatcaagaa gatcaaaaca ctatggtcca tggcgtaaaa
      241 aacgccacga accaggcagg ggatgttcag accgtcaatc ctggtcagcc agcaggcacg
      301 acaccggaag ccgattcagc gtatgcgctg aaagccgatt cgggcgctgt gcagcaggtg
      361 atgaccgcaa ggccggagca atcacaccaa ctgcagcagc ctgaagccga ttccgccatt
      421 cagcgggaag ccgatcgggt agtcccggaa aacaccgggc tgtctgtggg acgagtggat
      481 tatccggatg tgttcgaaca ggtctggcgg gagtacccgt tgcgtgccgg agcaaacccg
      541 aagaaatccg cattcagtgc ctggaaggcc agattgcgcg agggggtgcc accagagacc
      601 atgctggatg gtgtgaggcg ttacgcgaga tacctggcgg cgaccgggaa agcgggaacg
      661 gaatttgttc agcgagcgac gacgtttttt gggccggacc ggaattttga aaacccctgg
      721 ttgctcccgg taagcggcac gaacaaccag cgttgtgtga atcatatttc tgaaccggat
      781 accgaaattc cgccgggatt cagggggtga tgtggcatga aaaacattgc ggcagccggg
      841 gttcttgaac gtattcgcag acttgcacca ccggcgtcgg ttccaccgta ccggacggtg
      901 gaggagtggc gggaatggca acttg
//
LOCUS       DAZUGK010000492          925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZUGK010000492 DAZUGK010000000
VERSION     DAZUGK010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.495"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..>925
                     /locus_tag="ACHY5W_005056"
     rRNA            102..>925
                     /locus_tag="ACHY5W_005056"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          270 a          194 c          276 g          185 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgca agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgttt
      241 cgacacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgcgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttcgg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagct
//
LOCUS       DAZUGK010000493          917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZUGK010000493 DAZUGK010000000
VERSION     DAZUGK010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.496"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..537
                     /locus_tag="ACHY5W_005057"
     CDS             <1..537
                     /locus_tag="ACHY5W_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449749.1"
                     /translation="EAVSHIPVPRDGRDYDPDVLQKAVLDAVRALPAPQDGRDATALE
                     IIPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERSFTV
                     VVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAH
                     SSGWTLAAKRGRDAGGGK"
     gene            534..860
                     /locus_tag="ACHY5W_005058"
     CDS             534..860
                     /locus_tag="ACHY5W_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4449750.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT          200 a          221 c          309 g          187 t
ORIGIN      
        1 gaggcggtaa gccatattcc tgttccgcgt gatggtcgtg actatgatcc cgatgttctg
       61 cagaaggctg ttctggatgc ggtgagagcc ctgccggctc cgcaggacgg gcgtgatgcc
      121 acggcactgg aaataatccc cgccattgac gatcaaaaat cctttccccg gggcacgtat
      181 gccacacacc agggcggact ctggcgggcg tatgaaaaaa cgcacgggat gcggggatgg
      241 gaatgcctgg ttgacggggt ggccgatatt gacgtcagca tgaccggcga acggtcgttc
      301 actgtggtgg tccggcagag cagtggccag cgtacggaaa aaacattttc cctgccggtg
      361 atgctctacc gcggtgtgtt cagagccggt gaaacctacc accccggtga tacggtgacg
      421 tgggggggct cgctgtggca ctgcaacagt atgaccggtg ataaacccgg agaagctcat
      481 tcatcaggct ggaccctggc tgccaaacgt ggacgggatg caggaggcgg aaaatgacgg
      541 cattactgac actggaagag atcaaggcac atctgcgtgt cgaccatgac gcggatgatg
      601 acatgctgat ggacaaggtt cgtcaggcta ccgccgtgct gctggcctac attcagggca
      661 gccgggataa ggtgattcgt gaggacggtg aactgatccc gggcgaggca ttaacccgga
      721 tgaagggggc tgccatgcga ctgaccggga tgctgtaccg gaatccggat cttgcggagc
      781 gggaagaact gattcagggg gagctgccgt tttctgtgtc cgtgctgatt tacgatttgc
      841 gttgtccgac ggtgttatga ggagggagga atggcaatat ctgcaggtcg tctgacacag
      901 atgataagtg ttctgaa
//
LOCUS       DAZUGK010000494          915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZUGK010000494 DAZUGK010000000
VERSION     DAZUGK010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.497"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(227..877)
                     /gene="zinT"
                     /locus_tag="ACHY5W_005059"
     CDS             complement(227..877)
                     /gene="zinT"
                     /locus_tag="ACHY5W_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFS4449751.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWHGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          253 a          171 c          192 g          299 t
ORIGIN      
        1 agccaatata agcaatggcg tgagcacagg tatctttctc tgttggccgt attgtgtatg
       61 gaatccgtta ttggttaact caatgcgggt tagcttatta atatacagac acgtcatagt
      121 gatgagcgtg tctgtagcta gcttgcgttc agtggcgttt gtggatggac cagtggcagt
      181 tatgatgact tactaaagcg gcatcgaggc gttatcatga gaatactcaa tgagacatca
      241 tttcctcgac cacttcttcg ctactcaact gatatggata atacgttggc cagttttcca
      301 tttcatttaa taaagattgc tgtgaatcat tacccataaa aatgtgaaaa tgagatgatt
      361 ttcgtggtgc aattatatga tcgctaaatt gtatatattt aggggctttg ctttcaggat
      421 ctttacattc gaataagtaa cgaacgcctt tcttgcctga tttataggtg agtattttgt
      481 atccatcgta atcatattta caggatgttg tttcattatt tctatggaat tcaacaatgc
      541 cgtcctcaat gccaatcatc tcgatatctg ttgcataacc tttgtgataa taatctttaa
      601 tttcagcaaa tgttttagtt ttatctgcat ccgctttctt ctgaaagacg gggtcaagtt
      661 tgccactttg cagtaaagga taaacggatt gccagactcc atgccagtca ctgagcgttc
      721 ggttttgtac attggcatca tcaaaaacac cattagcagc tttttgttcg acctctgtta
      781 agggtttgcc gtgtgagtga tgaccatgcg aaaaggcagg agcgctaaca ataaagacac
      841 ctaaagcaac agccagtttg tgaagacgaa tcgccaaaac agtttcctcc agagttaatg
      901 tatatgatgt gtaat
//
LOCUS       DAZUGK010000495          911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZUGK010000495 DAZUGK010000000
VERSION     DAZUGK010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.498"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..141
                     /locus_tag="ACHY5W_005060"
     CDS             52..141
                     /locus_tag="ACHY5W_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449752.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            161..>911
                     /gene="nleL"
                     /locus_tag="ACHY5W_005061"
     CDS             161..>911
                     /gene="nleL"
                     /locus_tag="ACHY5W_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFS4449753.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKN"
BASE COUNT          279 a          147 c          194 g          291 t
ORIGIN      
        1 gaatatttgt tagataaaaa agaggagata attcaatagg gagttaaatt aatgccgata
       61 aatctgacat cttatttgtg ggtacaggga cagaaagttg tcccggcagt tgtgttttct
      121 aattttactt tagtggacta agtaaaaagg agtgagataa atgctgccca ctacaaatat
      181 ctctgtaaat tctggagtaa tatcttttga aagtcctgta gattcaccat ctaacgagga
      241 tgttgaagtt gccctcgaaa agtggtgcgc tgagggagaa tttagcgaaa atcgtcatga
      301 ggttgcatca aaaatacttg atgttataag tactaatgga gagactttat caatcagtga
      361 gccaataaca acattaccag acttgcttcc aggttctctg aaagaactgg ttttgaatgg
      421 atgtacagag cttaaatcaa taaactgctt accccccaac ttatcttcat taagtatggt
      481 tggatgctca tcattagagg ttataaattg cagcatacct gaaaatgtca ttaatttatc
      541 tttatgccat tgtagttctt tgaaacatat agaaggttcc tttcctgagg cactcagaaa
      601 ttccgtatat ttaaatggct gtaattcatt aaatgaatcg caatgtcaat tccttgcata
      661 tgatgtcagt caaggccgtg cctgcctgag caaagctgag cttactgctg acttaatttg
      721 gttgtcagct aaccgaacgg gtgaagagtc tgctgaagaa ttgaattact ctggatgtga
      781 cttgtcaggt ctaagtcttg tagggctgaa tttatcatca gtaaattttt ctggagcagt
      841 gcttgatgat acagatctca ggatgagtga tttgtctcag gctgtattgg aaaactgttc
      901 ttttaaaaac t
//
LOCUS       DAZUGK010000496          898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZUGK010000496 DAZUGK010000000
VERSION     DAZUGK010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.499"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..>898)
                     /locus_tag="ACHY5W_005062"
     CDS             complement(34..>898)
                     /locus_tag="ACHY5W_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4449754.1"
                     /translation="KDVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLRE
                     GKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTY
                     LAMRVAYFNELDSYAESLGLNTRQIIDGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQL
                     LANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQ
                     GIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVAD
                     KVYTRDLFGSD"
BASE COUNT          247 a          202 c          207 g          242 t
ORIGIN      
        1 aatcagggcg aaaatcgccc tgataacagg atgttagtca ctgccaaaga gatcgcgggt
       61 gtagacctta tctgccacat ccttaagctc ttctgccata cggttggaaa taatgacgtc
      121 ggcttgttgt ttgaaggtgg cgagatcacg ttccaggcga gagttaaaga atgagtcttc
      181 tttcatcacc ggttcgtaaa tgattacttc aacgcctttc gctttgatac gtttcataat
      241 cccctgaatg gaagacgcgc ggaagttatc tgaaccgctc ttcataatca gacgataaat
      301 acccaccact tgcggcttgc gtgacaaaat ggcatcggca ataaaatctt tacgcgtgcg
      361 gtttgcatcg acaattgccg agatcaggtt attcggcaca gactggtagt tcgccagtaa
      421 ctgcttggta tctttcggca aacaatagcc accataacca aacgacggat tgttgtaatg
      481 gttgccaata cgtgggtcga gacaaacgcc gtcgataatt tgacgcgtat tcagacctaa
      541 actttctgca tagctatcca gctcgttaaa gtacgctacg cgcatcgcca gataggtatt
      601 agcgaaaagt ttaatcgctt ctgcttcggt tgaatcggtg aacagggtcg ggatattttg
      661 cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcacgttctg aacgctcacc
      721 gatgacaata cgtgaaggat ggagattatc gtaaagagct ttaccttcac ggaggaattc
      781 gggggagaaa ataatatttt cagtacgata tttcttatgc atcgctgcgg taaaaccaac
      841 gggaaccgtg gatttgatga ccataaccgc ataaggattt atctctacta cgtcttta
//
LOCUS       DAZUGK010000497          896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZUGK010000497 DAZUGK010000000
VERSION     DAZUGK010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.500"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>896
                     /locus_tag="ACHY5W_005063"
     CDS             <1..>896
                     /locus_tag="ACHY5W_005063"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4449755.1"
                     /translation="EFGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMP
                     SQMAARLDEAWAAIKTGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANA
                     ECTIRTIKSFGSPFSAASGNRRPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFE
                     NKRAALAASLEEIMNKAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAA
                     TAQPVKQAGNGNVATVASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQ
                     YPDDSRLHHVLKSAVGAGTTTDP"
BASE COUNT          195 a          241 c          290 g          170 t
ORIGIN      
        1 gagtttggca gtacgatccc gtttctctgg cagcatgacc attcccgccc tgtaggccag
       61 tgtacggtgc gtcgggtcag ggaagggctg gaaatcacgg caatgctggt gaagccggag
      121 ccggggatgc cctcccagat ggcagcccgg ctggatgagg cctgggctgc cattaagacc
      181 gggctggtca gggggctttc tgtgggcttc cggccccatg aatacactta tctggacgga
      241 ggcggactgc attttctgcg ctgggagctg atggaggtgt ctgccgtcac cgtgcccgcg
      301 aatgcggaat gcaccatccg gaccattaaa tctttcggca gcccgttttc tgccgcgtcc
      361 ggcaaccgga gaccggtggt gaaaatcgca tcttctgccg gcgcttcggc acagtcaata
      421 acctcttttc ataaggaaaa gtcagcaatg aatactggtg aacagattaa aagttttgaa
      481 aacaagcgtg cggcgctggc agcctccctt gaggagatca tgaacaaagc cgcagaggaa
      541 ggccgcacac tggatgtgga agaggaagag cactacgaca acaccgcagc ggaaatccgt
      601 caggtggatg cgcacctgaa gcgtctgcgt gaactggaag ccggtaaggc cgccacggca
      661 cagccggtga aacaggccgg taacgggaat gtggccacgg tggcttcagc gccggtgatc
      721 cgtgttgagc agaaactgga gaaggggatt ggtttcgccc gctttgccaa atcactggcc
      781 gcggctaaag gtgtccgctc tgaagccctg gaagtggccc gtcgtcagta tccggatgac
      841 agccgtctgc atcatgtcct gaaatcggcg gtgggggcag ggaccaccac ggaccc
//
LOCUS       DAZUGK010000498          895 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZUGK010000498 DAZUGK010000000
VERSION     DAZUGK010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.501"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..82
                     /locus_tag="ACHY5W_005064"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHY5W_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(498..>895)
                     /locus_tag="ACHY5W_005065"
     CDS             complement(498..>895)
                     /locus_tag="ACHY5W_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NleF caspase inhibitor"
                     /protein_id="HFS4449756.1"
                     /translation="SNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGM
                     TIDDRDGYIRELFFLYSLKVKIEESRQGKEGSKCKVYGLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT          267 a          228 c          145 g          255 t
ORIGIN      
        1 atcctaatcc atgcatgacg tgtggtgcct gttgtgcatt tttcgcgtct ctttttactg
       61 ggctgaagca gatgatgctg gcgaaaggtt tatcagaaaa ccgtagcgcc ttcttgcata
      121 cgacgtgtcc gcagttttat ataatcccga ccagaaacct cttataaaaa gtcgatacac
      181 aaatatcatg gataatcacc accttctgac gaagaatatc tgatgaaatt aatcgcccct
      241 ctcctgagac cattgcgctg gggtaaattt gtaactacgt gtgtacagtg aggtgtcgta
      301 tagcacgccg tcaggaaatg caaggtgccc ccgatatccc cgactacaag tcagcaaaca
      361 aagtgattgg ctggcaatcc acatcacaga aatggacaat gctactttat aatcgagctt
      421 ggtagccacc ttgatggtca cttttaaaaa aagcgtttac acaaaatgat aagcaggctc
      481 acaggacctc cctctaatca tccacattgt aaagctcctt tgttgtaagt aagatccggc
      541 accctaataa tatcaaaagg atcaaaatca tcactgcatc cttccacaag acatttcatt
      601 gctcgtagat caccatatag ctctgaagag tgatgcggac ataacagacc ataaacttta
      661 catttagaac cttctttacc ttgtctactt tcctcaattt ttactttcaa tgaatacaga
      721 aaaaaaagct ccctgatata accatccctg tcatctatcg tcatcccctc cctcagtgct
      781 ccgacaacct catcagtatt ttcatttctc acctcaaata cactatttct cttatccatc
      841 agcccactta caaaagcact tagctcttga taaatttcct caagctcatt actat
//
LOCUS       DAZUGK010000499          886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZUGK010000499 DAZUGK010000000
VERSION     DAZUGK010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.502"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..270)
                     /locus_tag="ACHY5W_005066"
     CDS             complement(16..270)
                     /locus_tag="ACHY5W_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4449757.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            380..532
                     /locus_tag="ACHY5W_005067"
     CDS             380..532
                     /locus_tag="ACHY5W_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFS4449758.1"
                     /translation="MEKEVSRILVRMPQSLKDAITGKAKEECRSFNSEVIKRLLDSLK
                     REGITV"
     gene            796..>886
                     /locus_tag="ACHY5W_005068"
     CDS             796..>886
                     /locus_tag="ACHY5W_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFS4449759.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEP"
BASE COUNT          266 a          162 c          203 g          255 t
ORIGIN      
        1 ggattatgcc caccatcagg tgggtttttt atcttcgccg tacatgctgc tgagagtctt
       61 aagaagagcc tccctaaatt tttcagattc ttgttgagca aaaccttcta ttgatcttgg
      121 tgatttttct tcatcaatag cagcttgcaa gatcataact atttctgaat ttaatgagcg
      181 tccgtttttc gatgctctta tggtcaggtc gcgcttgagg ctatctggca ttcttacgct
      241 atatggggct atgtctctaa ccttcgtcat acttacaccg tgataatcac attgatatca
      301 cagtgtattc aaaaaaactt tgacttgata tagtcacagt gatacgtttg tgattcataa
      361 taactcacgc tgtggcgata tggaaaaaga agttagcaga attttggtga gaatgcctca
      421 gtcgctaaaa gatgcgatta caggaaaggc caaagaagaa tgccggtcgt ttaactcgga
      481 agtgattaag cgattgctgg acagcctgaa gagagagggg ataacggtat gagtaaagaa
      541 tgttgttttt tgcggcatta gcgaatcaga cgctgatcaa acatacattt actctaaaga
      601 aacaggtcgg atgctgtgta gtgactgcgt gtttgacatc ataagataca agtatcttgg
      661 atgttctgcc agcattagca atataggtga agtgtacgaa gggaaagata taactgatag
      721 agcagaaagt tgaagcccca actgctgtaa cagtcagggc ttcgttatca acaaatcggc
      781 ttaggaaata ttgacatgaa aagtatagca aaggcacaaa acgatttcac tatcttcaaa
      841 ttcggcgaca gtgaaatccg cgtcatcaac aagtgcggtg agccgt
//
LOCUS       DAZUGK010000500          881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZUGK010000500 DAZUGK010000000
VERSION     DAZUGK010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.503"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..881
                     /locus_tag="ACHY5W_005069"
                     /pseudo
     CDS             1..881
                     /locus_tag="ACHY5W_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
BASE COUNT          301 a          141 c          177 g          262 t
ORIGIN      
        1 atgtttccat taaatgatct gtcgttaaaa actcagtcgg ttcaattaaa taaaatcaca
       61 tctaatactg agtctacgat aaaacaacaa gagttagtat ctgatgatgc aatcataaat
      121 gaattatcaa gtgagttagt cagttgtctt ggaaatggta agtttacacc aattagtgaa
      181 ggcagcaaat tattgaatat gctgtcagaa tttaagttat tgagagagta atgtttcagg
      241 tggggtaatt atactctatt gtttgaaaat tatggggatt atgataagac ggggtctatc
      301 acgatagaaa aaagtcaggg ggaggggact ttacccattc gacataaatt agagtttata
      361 tcgaccaata ttgcagagtt gctggacaag ttaaccaaaa ttacagatgc caggctttgc
      421 aaaggtttca gtgactgggc tagttcagtc aaagaaggcg catcgaatga cctcaaagaa
      481 aatgtggata gagcattggt gagaatgttt aaatgtgtta agcttcacag taatgaactt
      541 aacttatcaa gcctttcttt gggttctgtg ccgcctcttc cagagtggat tgaaatactt
      601 agccttgttt ataatgaact tgattcaata caggtgcccg aatcatgcaa agaattagaa
      661 ctcgatttca ataaccttac agaatttcca caagtacctg atggaattac cctgatctcc
      721 gtaaataata acctgatatc gcatattgac tcatttccgc caaaggctaa gaaaattttt
      781 attggtcaca ataagttatc ggaaatacca gcaataccag acaccgctaa ggtttttgat
      841 tgcagtgaga ataatataaa agaaattaga tggttcccca a
//
LOCUS       DAZUGK010000501          874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZUGK010000501 DAZUGK010000000
VERSION     DAZUGK010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.504"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..829
                     /locus_tag="ACHY5W_005070"
     CDS             <1..829
                     /locus_tag="ACHY5W_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449760.1"
                     /translation="KTILIRRKVSRNTYKIRVNQLETIKEKLGGILLTEITTRHIAEF
                     LDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKTY
                     NCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRLHIRQIKTGMMIAIP
                     LSLSLPVAGLRLGTVVERCRLVSRGDFLISAGMRKNSPDGSIHPDGLTKKFVAARKLT
                     GIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT          292 a          174 c          198 g          210 t
ORIGIN      
        1 caaaacgatt cttataagga gaaaagtgtc cagaaatact tataaaattc gggtaaatca
       61 actggagaca ataaaagaaa aattaggagg aattttactg acagaaataa ccactcgcca
      121 tattgccgag tttcttgatt tgtggattga aggagggaaa aacacgatgg caggatcaat
      181 gcgttctgtg ttatctgata tgttccgcga agccattgtt gaaggacgta tatctcaaaa
      241 tccagtaacg ccaacaagag caccgaaaat agtagttaca agagaacggc tgaaactaaa
      301 gacatacaac tgcatcaggg aggcagcaga tcaacttccg gcatggttcc cattggctat
      361 ggacttagcc cttgtaacag gacaacgtcg cgaagacata acgaatatgc ggttcagtga
      421 tatttatgat gatcgcctcc acatcaggca aattaagaca ggaatgatga ttgctatccc
      481 cctgtcactc agccttcctg tcgctggtct acgtcttggt acagtagttg aacggtgccg
      541 cctggtaagc cggggagatt ttctaatcag tgccgggatg agaaaaaaca gccctgacgg
      601 cagcattcac ccggacggcc tgacaaaaaa atttgtcgca gccagaaaat taacaggtat
      661 ccagttcagt gaaaacccac caacttttca cgagatcaga agcctggctg gacgattata
      721 caaagaaaca tgtggagaag aatttgctca gcgtctactt ggccacacat cggagaagac
      781 aacaaaaatg tatcttgatg agagagaaaa aacgtactta ctgctctgat tttaacgtaa
      841 atggattgtt aaatgtattt tagttgtgat ataa
//
LOCUS       DAZUGK010000502          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZUGK010000502 DAZUGK010000000
VERSION     DAZUGK010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.505"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(54..278)
                     /locus_tag="ACHY5W_005071"
     CDS             complement(54..278)
                     /locus_tag="ACHY5W_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449761.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(369..445)
                     /locus_tag="ACHY5W_005072"
     tRNA            complement(369..445)
                     /locus_tag="ACHY5W_005072"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Arg,seq:tct)
     gene            complement(459..535)
                     /locus_tag="ACHY5W_005073"
     tRNA            complement(459..535)
                     /locus_tag="ACHY5W_005073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Arg,seq:tcg)
     gene            complement(543..618)
                     /locus_tag="ACHY5W_005074"
     tRNA            complement(543..618)
                     /locus_tag="ACHY5W_005074"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Ile,seq:cat)
     gene            complement(659..772)
                     /locus_tag="ACHY5W_005075"
                     /pseudo
     CDS             complement(659..772)
                     /locus_tag="ACHY5W_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
BASE COUNT          238 a          217 c          184 g          217 t
ORIGIN      
        1 tatttatctc cttgtgggta cccagagata ttttatgatt gctcccggtc agatcaataa
       61 agtggcttca attttgcctt aatgatcaaa tcagggtgat tgacggaatc gtacaccacc
      121 tcaatatttt catccgtggc gtcgataaga tattctttta cataaggacc tgttgatttt
      181 ccatgaaata catcttcaac aagtacactc tccccctgaa caacacgaaa actaacttct
      241 gtttcgaacg gaccaatcgt caccatcagt tttttcacat agcctcctga taagcactcg
      301 atttattagt taatggtgta acgcagatac aaaaaaaggc ccgcaaaagc gagccaagta
      361 aataaatatg gcgcgttgta ctggattcga accagtgacc gattgcttag aaggcaattg
      421 ctctgtccga ctgagctaac aacgcatgat gctgataatg gaccgccatc ggggacttga
      481 accccgcaca gccagcttcg aaggctgacg ctctatcccg atgagctaat ggcggtttgt
      541 gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
      601 ccactgagct aaagggccgg gcgcaggata ataacggtac gtaactaatc ctgcaatatc
      661 atccgttctg actgactaaa tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct
      721 ccagctcaac gccaattgca cgacgcccca gcgacattgc tgctttgacg ctacagacat
      781 aaaaaagcca gccactgggg gaggctggca aactcgtaga gcaaaatgct gttacgcaaa
      841 cttcgttaca gggtca
//
LOCUS       DAZUGK010000503          848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZUGK010000503 DAZUGK010000000
VERSION     DAZUGK010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.506"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..800
                     /locus_tag="ACHY5W_005076"
     CDS             15..800
                     /locus_tag="ACHY5W_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449762.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESPIDEKKKKTQTAQSSINKNVP
                     QQLTDKDLYGNETTLVVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRQKPVTCNKDKNKDESLDVVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          272 a          143 c          197 g          236 t
ORIGIN      
        1 gggagtgtcc gattatgaat ttaagaaggg tttgttgtat atgtgtatta ttctcgctgc
       61 ttgcaggatg tgcctctgaa tctcctattg atgaaaaaaa gaaaaaaact caaaccgcac
      121 aaagttctat taataaaaac gttccccagc aactaacaga caaagattta tatggtaatg
      181 aaaccacact ggttgtatct gaggaggata ttcaagctgc gcttgaagga gatgagtttc
      241 gtgtgcctct taattctccc gttatacttg ttcagtctgg cagtcgcgca ccggaaacca
      301 ttatgcagga ggagatgaga aaatattata cagttgccac attttctggc attcctgaca
      361 ggcaaaaacc tgtaacttgt aataaagaca aaaacaaaga tgaaagtctg gatgttgtag
      421 atgccgaaaa tatgaactgg atgcaggcat tacgttttgt tgcggcaaaa ggccatcaga
      481 aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct ggattgaaat
      541 caatagtttg gtcagattat aaaaatcaaa aattgactga cacgatgtca ctgcgttatt
      601 tggttcgatt cacgctggtg gatgtggcta caggtgagtg ggctacctgg tcgcctgtga
      661 attatgaatc gaaagtgatt tttccgcaga tagggaataa aaattctgac agccttgatg
      721 tggcagaaca gcagatttca caacttaagc agaaaacgta cgcagcagtg gttaaagatc
      781 tggtgaatcg ctatcagtag taaggtttta taccagacgt tttaatggta cttagcaaga
      841 aagagcat
//
LOCUS       DAZUGK010000504          845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZUGK010000504 DAZUGK010000000
VERSION     DAZUGK010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.507"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..188)
                     /locus_tag="ACHY5W_005077"
     tRNA            complement(112..188)
                     /locus_tag="ACHY5W_005077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(152..154),aa:Arg,seq:tct)
     gene            complement(202..278)
                     /locus_tag="ACHY5W_005078"
     tRNA            complement(202..278)
                     /locus_tag="ACHY5W_005078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Arg,seq:tcg)
     gene            complement(288..363)
                     /locus_tag="ACHY5W_005079"
     tRNA            complement(288..363)
                     /locus_tag="ACHY5W_005079"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Ile,seq:cat)
     gene            complement(392..>845)
                     /locus_tag="ACHY5W_005080"
     CDS             complement(392..>845)
                     /locus_tag="ACHY5W_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4449763.1"
                     /translation="ALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSGCLS
                     SISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAV
                     NMLCSRHGYGQAIAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          237 a          223 c          191 g          194 t
ORIGIN      
        1 gtcacactga ccactccgta agctcacccc cgaaaggctc tgtggttgat atgcgccggg
       61 cgtggcgcag atacaaaaaa ggcccgccga agcgagcctg gaaaataagt gtggcgcgtt
      121 gtagtggagt cgaaccactg accgattgct tagaaggcaa ttgctctgtc cgactgagct
      181 aacaacgcat gatgctgata atggaccgcc atcggggact tgaaccccgc gcagccagct
      241 tcgaaggctg gcgctctgtc ccgatgagct aatggcggta tgtgatatgg tggcccttgc
      301 tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg
      361 gccgagccaa aaaataataa tcagatgaaa atcaataatc aagcccttgc ctggatacat
      421 atctgtctgg cgggaagcca taatagcggt gaaatacaga aataaagtag gacctgcttg
      481 aataaccgca tttttctgct atagcctgtc catatccatg ccgggaacat aacatattta
      541 cagcaacacg catccgctct tccagcaaca agcgactgaa catgccccct tcatttttca
      601 gttttgtctt taacaaactc tcactcatat gcaactgtag agcaatcgca ccaagcgtcc
      661 agcttgctga tatatctgtc tgaattatcg ccctgacttt ggcacttatg ctggataaac
      721 atccacttaa aaacaatgac atccgttcat ctgtttcaaa cagagacagg caggccatca
      781 taagaaacat atccgtgacc tctccggaaa atccctggct ggtaattaaa gccgcagcca
      841 acgca
//
LOCUS       DAZUGK010000505          844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZUGK010000505 DAZUGK010000000
VERSION     DAZUGK010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.508"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>844
                     /locus_tag="ACHY5W_005081"
     CDS             <1..>844
                     /locus_tag="ACHY5W_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFS4449764.1"
                     /translation="SVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHE
                     PSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRC
                     KQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDV
                     YTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQF
                     GLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRR
                     HSRLG"
BASE COUNT          236 a          202 c          225 g          181 t
ORIGIN      
        1 cagcgtattt tgcacaggag tcgctgaaaa atacgcgtta atcgaacaat ggcgacaaca
       61 atttcccatt gaagcgatgt gtcaggtatt tggtgtatcc aggagcggtt attacaactg
      121 ggtacagcat gaaccctcag acagaaaaca aagtgatgag cggctaaaac tggagattaa
      181 ggtggcacat atccgcactc gcgaaacata tggaacccgg cggctccaga cggagctggc
      241 agagaatggc atcatcgttg gtcgtgaccg actggcacgt cttcgtaagg agctaaggct
      301 acgctgtaag cagaaacgca agttcagagc gactacgaac tcgaaccaca atctgccagt
      361 tgcgccaaat ctgctgaacc agacgttcgc tcctacagca ccaaatcagg tctgggtggc
      421 ggacctgacg tatgttgcca cacaggaggg atggttgtac ctcgctggca tcaaagatgt
      481 ttatacgtgc gaaattgtcg gctacgccat gggagagcgc atgacaaaag agctgacagg
      541 taaagctctg tttatggcgc tcaggagcca gcgcccacct gccgggctaa tccaccactc
      601 tgatcgaggt tcacagtact gcgcatacga ttaccgggtc atacaggagc agtttggtct
      661 gaaaacatca atgtcgcgta aaggtaactg ttacgacaac gctccgatgg aaagcttctg
      721 gggaacgctg aaaaatgaga gcctgagcca ctatcgtttt aataaccggg atgaagccat
      781 ctcagtaata cgggaataca ttgagatttt ctacaatcgt cagcgtcgtc actctcgtct
      841 gggg
//
LOCUS       DAZUGK010000506          843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZUGK010000506 DAZUGK010000000
VERSION     DAZUGK010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.509"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..737)
                     /locus_tag="ACHY5W_005082"
     CDS             complement(<1..737)
                     /locus_tag="ACHY5W_005082"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4449765.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWL"
BASE COUNT          238 a          211 c          170 g          224 t
ORIGIN      
        1 agccagccgg aatgttcccg ctctttggtg ctgatacgcc ctatgcgtaa tgaaccattt
       61 tcaggacagt tcattcttct ttcggttgta tataaataag agaaaccaag cttaccggcg
      121 agattaatat aatcccgatt ataatatcct tcaggccagc acagatggga actacagaac
      181 cccagctttt ccgtcagaca ttgttttccc accagaatat cttctttcat gagccggcac
      241 tgttcagcac gtgatacaga caacctgtcc cagcgtttat gtgaatgggt gtgcacatga
      301 aattcaacca gtccggaatc acgcatttcc cggacttcag accagcgaag catgacctca
      361 tctgaacggt tatcggcaat caaccgttca cagtcgcggt gagaatattc ctgctcctgc
      421 ctgctacgta cgtttccctt accaatcagc cccgtaatga gaaaaatatg tgcatgaagg
      481 ttatattcct tcaggaccgg ccatgctctg agccagttat caagataacc atcatcgaat
      541 gtgagcatga cactctttcg tggaagcgtt cccccctgat aaaaatattc aagctcagca
      601 gatgttaccg tccgccagtt attatctgcg agccacttca tctgttcaca aaatgtttca
      661 ggtgagagtg tcacaagccc cggacaacga ctgacatgat gatacatcag tacgggaaga
      721 tgccttgcat tcaacataaa aaattatcgt ctgttaagca ttctgaaaat atcctcgcgt
      781 aaacaacagt gaggatccat agatatcaca catggtgata ttattgtgta atcccggaat
      841 ggt
//
LOCUS       DAZUGK010000507          841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZUGK010000507 DAZUGK010000000
VERSION     DAZUGK010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.510"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>841
                     /locus_tag="ACHY5W_005083"
     CDS             <1..>841
                     /locus_tag="ACHY5W_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4449766.1"
                     /translation="GSGEDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGE
                     PATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVET
                     SARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFR
                     EKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQ
                     RVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINP
                     ENGN"
BASE COUNT          223 a          213 c          248 g          157 t
ORIGIN      
        1 ggaagcggag aagacagccg tctggtgacc accgccatca ctgcggatac agagcatcgt
       61 ttcagtggtc tgccgcccgg ggaatacacc ctgacagtca gggcaattaa cagttatggc
      121 cagcaggggg aaccggccac caccaccttc cggattaacg cgccagcaaa acccgccacc
      181 attgaactga caccgggcta ttttcagata acggcggtcc cgcgtcttgc ggtgtatgac
      241 ccgacggtac agtttgagtt ctggttttcg gaggcaaaaa tcgcagacac atctcaggtg
      301 gaaacctctg cccgttatct ggggaccggc agtcagtgga gtgtatccgg cccgcacatt
      361 aagcccggga aggatttctg gttttacgtg cgcagcgtca acctggtggg gaaatctgcg
      421 tttgtggaag tcagcgggca gcccagcaat gatggtgaag ggtatctgga atttttccgg
      481 gaaaaaatag gaaaactgca tctggctcag gggctgtggg agctgataga caacagccag
      541 cttgcggatg agatggcgga gatgaagacc accatcaccg aaacccgcaa tgaaatcaca
      601 cagacggtca gtaaaacgct ggaagaccag agcgccacca ttcagcagat acagcgcgtg
      661 cagaaggaca caaatgatga cctggctgcg ctgtacatgc tgaaggttca aaaaacgaaa
      721 gacggcattc cctatgtggc cgggattggt gcagggattg aggatactga tggccagcca
      781 ctgagcaaca tactgctgct ggctgaccgt atcgcgatga taaatccgga gaacggcaac
      841 a
//
LOCUS       DAZUGK010000508          841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZUGK010000508 DAZUGK010000000
VERSION     DAZUGK010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.511"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..365
                     /locus_tag="ACHY5W_005084"
     CDS             <1..365
                     /locus_tag="ACHY5W_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFS4449767.1"
                     /translation="SLYAEHELEHALNETCEALVRAMHLSILVQENPLANTTGHQGYV
                     APEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAGTPAQRKVW
                     QDKIDQQGAELKARGLLS"
     gene            362..>841
                     /locus_tag="ACHY5W_005085"
     CDS             362..>841
                     /locus_tag="ACHY5W_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFS4449768.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTITRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     "
BASE COUNT          225 a          214 c          227 g          175 t
ORIGIN      
        1 tgagtctgta tgcagagcat gagctggaac acgccctgaa tgaaacctgt gaggcgcttg
       61 tccgggcaat gcatctgagc atcctggtac aggaaaatcc gctcgccaac accaccggcc
      121 atcagggcta tgtcgcaccg gaaaaggctg tcatgcagca ggtgaaatca tcgctggaac
      181 agaaaattaa acagatgcaa atcagcctca ccggtgagcc ggttctccgg ctgaccggac
      241 tgtcagcggc aacacttccg cacatggatt atgaggtggc aggcacaccg gcacagcgca
      301 aggtgtggca ggacaaaata gaccagcagg gagcagagct gaaggccaga ggactgctgt
      361 catgatttac tgcccgtcgt gtggacatgt tgctcacacc cgtcgcgcac atttcatgga
      421 cgatggcacc aagataatga ttgcacagtg ccggaatatt tattgctctg cgacatttga
      481 agcgagtgaa agctttttct ctgactgtaa agattcagga atggaataca tttcaggcaa
      541 acagagatac cgcgattcac tgacgtcagc ctccggcagt atgaaacgcc cgaaaagaat
      601 gcttgttacc ggatattgtt gtcggagatg taaaggcctt gcactgtcaa gaacatcgcg
      661 gcgtctgtct caggaagtca ccgagcgttt ttatgtgtgc acggatccgg gctgtggtct
      721 ggtgtttaaa acgcttcaga ccatcacccg cttcattgtc cgcccggtca cgccggacga
      781 actggcagaa cgcctgcatg aaaaacagga actgccgcca gtacggttaa aaacacaatc
      841 a
//
LOCUS       DAZUGK010000509          833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZUGK010000509 DAZUGK010000000
VERSION     DAZUGK010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.512"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            34..567
                     /locus_tag="ACHY5W_005086"
     CDS             34..567
                     /locus_tag="ACHY5W_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449769.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            617..>833
                     /locus_tag="ACHY5W_005087"
     CDS             617..>833
                     /locus_tag="ACHY5W_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS4449770.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTITPEKIQVPFIL"
BASE COUNT          267 a          159 c          163 g          244 t
ORIGIN      
        1 tgaccttcca taccagttaa ttaaggatga tttatgaaaa agtatattat tcccttcgtt
       61 gcgacaacgt tgatgtttag cgcaacaacc ttcgccaagg atgtcgatca aggaacacta
      121 accattagag gagaagttgt tgatacaacg tgttactttg ttaatggtga ttcgacaaca
      181 gatatcgttg tcgaagatgt tgtaaccagt gcaatgaatc aacttatcaa taatgaaatc
      241 tatactttaa aaaccggttc aacctctcat gacttagaaa ttcagtgtga cggaaataca
      301 gccccaagta tcgaagtact agcatcagag tttgatgcta ataactttac gctgaataaa
      361 ggaactgctg agaacattgg ctttgccatt ttcctcgatg atgggacttt gaccagaatg
      421 agaccagggt taaaaacacc gctgaaagat aatggctctg gtcaatattt tcttaatttg
      481 aatgcacaat atgcccgcac aagtggcaat cctgttacaa aaggctccgt agagtcaacc
      541 gtaacgatta aaatcagtgc agattaacgt tttttgccgg tagctctgct accggtatta
      601 tttacggagt gattacgtga gaataataac agcattactg atgtcatttt ttattttacc
      661 tgttcatgcc ggtgtggtca tctatggtac gcgcattatt tatccggaaa aaaacagaga
      721 agttctcgta cagttaatga atcaaagtaa aaatgcctct ttaattcagg catggatcga
      781 tgatggaaac accactatca cgccagaaaa aattcaggtc ccatttattc tca
//
LOCUS       DAZUGK010000510          832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZUGK010000510 DAZUGK010000000
VERSION     DAZUGK010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.513"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            242..448
                     /locus_tag="ACHY5W_005088"
     CDS             242..448
                     /locus_tag="ACHY5W_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS4449771.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKAVRGE"
     gene            453..797
                     /locus_tag="ACHY5W_005089"
     CDS             453..797
                     /locus_tag="ACHY5W_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFS4449772.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
BASE COUNT          214 a          159 c          239 g          220 t
ORIGIN      
        1 tggatttcac atgatactta tgcctggcgg tatattttct gacagacagt gacggatgtt
       61 gtcaagatat tgtgtcattt ataacctgaa ccaggggggc cggaatgtta tctggcattt
      121 ttagcagagc ctgaatgcca taatcacggc tcccggagtt ggccgtcagt gggtgacact
      181 ggcggctttt tgttttcctt tactttcatt ttctgtcggc ggtgacggag acatacatca
      241 gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtaggga cgggatactg
      301 gttactgcaa ctgctggaca aagtctctcc gtcccagtgg gtggcaatag gtgtactggg
      361 gagtctgctg tttggcctgc tgacgtatct gactaacctg tatttcaaaa tcagagagga
      421 ccggcgtaag gcggtgcggg gagagtaaag tgatgaagaa aaaatacgaa ctggttgtta
      481 aagggataaa taattacccg gataagatta ctgttactgt ggcactggaa attggtgggt
      541 atccgtcact gttgttgcca gatgtggcga ttagtcttga ccgtactgaa ggtgccacgc
      601 tggagtttta cgaagctgag gcgaaaaagc aggcgaagca gtttttcatg gatgttgctg
      661 ccgggttatg tgaaggggat ggtccgttgc cggaaaagcg gcccatcatt ttagaggcgc
      721 aggatgtgtt gataacctac agaggaaaac taccgggaat aattacgggt tctctgaaga
      781 ctccaccgct ggcctgaaga cttaacatat ccagggattt gaaatcgata aa
//
LOCUS       DAZUGK010000511          828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZUGK010000511 DAZUGK010000000
VERSION     DAZUGK010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.514"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            304..465
                     /locus_tag="ACHY5W_005090"
                     /pseudo
     CDS             304..465
                     /locus_tag="ACHY5W_005090"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            449..514
                     /locus_tag="ACHY5W_005091"
     CDS             449..514
                     /locus_tag="ACHY5W_005091"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFS4449773.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            630..>828
                     /locus_tag="ACHY5W_005092"
     CDS             630..>828
                     /locus_tag="ACHY5W_005092"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449774.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDW"
BASE COUNT          243 a          191 c          199 g          195 t
ORIGIN      
        1 agacacgaaa aacaactttt tatctttttg tttcgctcaa attagtataa aaagccgaac
       61 aacaaacaat aaaaaaccat taacatcaat agattatgat ggcttcaatc taaataatag
      121 gctatataat actgcaagac acaacacatg ctgtcactat gagtcaacta tttagatgat
      181 attagtgacc tgtttcagag cattagcgca aggcgattct ttgtcttctt gcactaattt
      241 tttatcataa aaatgttcct agcactgggc atcaatatcg caggtcagaa agagctcctg
      301 gggatgcggc tggccgaaaa tgaaggggcg aatttctggt tcaatgtgct gactgaactg
      361 aaaaaccgcg gtctgaacga tatcctcatc gcctgtgtgt atggcctgaa agaattcccg
      421 gaggcccgca tccagttatg catcgtgcat atggtgcgca acagcatgcg cttcgtgtca
      481 tggaaggaat acaaagccgt cactcgcgac ctgaaagcga ttagcctccc acagaagagg
      541 caggccagca ggcactggaa gcgtttgctg cggcctggga ctgccgctat ccgcagataa
      601 gccggtgcta gctgtcaaac tggactaact tggcgacgtt tttcgcttat ccggcagata
      661 tccgcaaagt gatctacaca acgaacgcca tcgagtcgct gaacagtgtg atccggcatg
      721 ccatcaggaa acgcaaggtg ttcccgacgg acgagtcagt aaaaaaagtg gtgtggctgg
      781 caatccaggc cgcgtcacag aaatggacaa tgccgttaag ggactggc
//
LOCUS       DAZUGK010000512          823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZUGK010000512 DAZUGK010000000
VERSION     DAZUGK010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.515"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            190..792
                     /gene="fimB"
                     /locus_tag="ACHY5W_005093"
     CDS             190..792
                     /gene="fimB"
                     /locus_tag="ACHY5W_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFS4449775.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          236 a          156 c          186 g          245 t
ORIGIN      
        1 tagcgtttgt atggcaacgt tattataatt aactgttgct actccattta agttcactca
       61 gaagaactgg tccacttacg ttagttatta aacaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaaacga tgaagaataa ggctgataac aaaaaaagga acttcctgac ccatagtgaa
      241 atcgaatcac tccttaaagc agcaaatacc gggcctcatg cagcacgtaa ttattgtctg
      301 actttgcttt gttttattca tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg
      361 gatattgatc ttaaggcaaa gtgtatatat atccatcgat taaaaaaagg cttttcaaca
      421 acgcacccgc tattgaataa agaagttcag gctttaaaaa actggttgag tatccgtact
      481 tcgtacccgc atgctgagag cgagtgggta tttttatcgc gtaaggggaa tccgctttct
      541 cggcaacagt tttaccatat tatctcgact tccggtggta atgccgggtt gtcactggag
      601 attcatccgc acatgttacg ccattcgtgt ggttttgctt tggcgaatat gggaatagat
      661 acgcgactta tccaggatta tcttgggcat cgcaatattc gtcatactgt ctggtatacc
      721 gccagcaatg cagggcgttt ttacggcatc tgggatagag ccagaggacg acagcgtcac
      781 gctgttttat agagatagtt gagataccag ggatggtgtt aaa
//
LOCUS       DAZUGK010000513          805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZUGK010000513 DAZUGK010000000
VERSION     DAZUGK010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.516"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            211..>805
                     /locus_tag="ACHY5W_005094"
     CDS             211..>805
                     /locus_tag="ACHY5W_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFS4449776.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLAS"
BASE COUNT          179 a          242 c          207 g          177 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgtagc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgatcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgc
//
LOCUS       DAZUGK010000514          803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZUGK010000514 DAZUGK010000000
VERSION     DAZUGK010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.517"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..>803
                     /locus_tag="ACHY5W_005095"
     CDS             58..>803
                     /locus_tag="ACHY5W_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HFS4449777.1"
                     /translation="MLRPRIIPCLLIHDSGLVKTVNFKSPKYVGDPINAVKIFNEKEA
                     DELMVLDIDATSRGLEPNYDLIKKLAAECRMPLCYGGGVTSVAQATKIISLGVEKVSI
                     SSAAVENPNLVRELAEAVGKQSVVVVLDIIKRKGLFSKGYELSTRNNTRKHKIDPVSF
                     AKEMADLGAGEIVINFVDNDGVMGGYDVAYCSTIKSQINIPVTFLGGAGSYDHLSALI
                     DQCGIVGAAAGSLFVFKGKYRAVLISYPTP"
BASE COUNT          250 a          143 c          179 g          231 t
ORIGIN      
        1 agaaaagcca ttcatcggga tcccaactac taaaaaattt tgcagagatc taatagcatg
       61 cttagaccaa gaatcatacc ctgtttactt atccacgatt ctggcttagt caaaacagtt
      121 aattttaaat cccctaagta tgttggtgat ccaattaatg ccgtcaaaat tttcaatgag
      181 aaagaagctg atgaattgat ggtcctagat attgatgcga catccagggg actcgaacct
      241 aattatgatt taataaaaaa actcgcagcg gaatgccgta tgcctttatg ctatggagga
      301 ggcgtaacca gcgtagcaca ggctacaaaa attatctctc ttggtgttga aaaggtatca
      361 ataagttcag cagctgttga aaatcctaat cttgttagag aattggcaga agcggttgga
      421 aaacaaagtg ttgttgttgt gctggatatt atcaaacgaa aaggtctatt ctcaaagggc
      481 tatgaacttt cgacaaggaa taacactcgc aagcataaaa tcgatcccgt ctcgtttgca
      541 aaggaaatgg ccgatctggg agctggagaa attgtcatca attttgtaga taatgatggt
      601 gtcatgggag gatatgacgt tgcatattgc tcaactatca aatcgcaaat aaatattcct
      661 gttacgtttc tcgggggcgc tggtagttat gaccatcttt ctgcactgat tgaccaatgt
      721 ggcatcgttg gtgcggcagc tggtagtctt tttgtattta aagggaagta tagggcagta
      781 ttgataagct atccaacacc tga
//
LOCUS       DAZUGK010000515          801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZUGK010000515 DAZUGK010000000
VERSION     DAZUGK010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.518"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..690
                     /locus_tag="ACHY5W_005096"
     CDS             <1..690
                     /locus_tag="ACHY5W_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFS4449778.1"
                     /translation="AMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKSTAGISIS"
BASE COUNT          202 a          188 c          219 g          192 t
ORIGIN      
        1 gcgatgataa atccggagag cggcaacagc acgccgttat ttgtggcgca ggggaatcag
       61 ctgttcatga acgacgtgtt cctgaagcga ctgtttgcgg tgagtatcac gtcatccggc
      121 aaccccccga cgttttccct gacgccggag ggcaggctga ccgcaagaaa tgctgatatc
      181 agcggtaacg tgaatgcgaa ttccgggacg ctcaacaacg tcacgattaa cgagaactgt
      241 cgggttctgg gaaaactgtc cgcgaaccag attgaaggcg atctcgttaa aacagtgggc
      301 aaagctttcc cccgggattc ccgtgcaccg gagcggtggc catcagggac cattaccgtc
      361 agggtttatg acgatcagcc gtttgaccgg caaattgtta ttccggcggt ggcattcagc
      421 ggcgctaaac atgagagaga gcatactgat atttactcct catgccgtct gatagtgcgg
      481 aaaaacggtg ctgaaattta taaccgtacc gcgctggata atacgctgat ttacagtggc
      541 gttattgata tgcctgccgg tcacggtcac atgacgctgg agttttcggt gtcagcatgg
      601 ctggtaaata actggtatcc cacagcaagt atcagcgatc tgctggttgt ggtgatgaaa
      661 aaatccacag caggtatcag tatcagctga attttataac ccagaacggg cgtcagaaat
      721 gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgccgccatt
      781 ttgtccgcag ccatttgtct g
//
LOCUS       DAZUGK010000516          797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZUGK010000516 DAZUGK010000000
VERSION     DAZUGK010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.519"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>797
                     /locus_tag="ACHY5W_005097"
     CDS             <1..>797
                     /locus_tag="ACHY5W_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4449779.1"
                     /translation="FDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQFN
                     DPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIHGPQLISCS
                     NNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVISRSVICMPLH
                     TIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAAVDDIQMEIDERFMNRKISFR
                     GSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKL"
BASE COUNT          250 a          177 c          139 g          231 t
ORIGIN      
        1 ttcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag cattagcaaa
       61 gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca acgcttacgc
      121 gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca
      181 gggttaaatc tgcatcacca tcttgagaaa aatctcagag gccttgaaca aacaattaat
      241 atagtcaata agtccgaact aaagaaaaac tttattattc acggccctca gctcatttct
      301 tgctctaata atagtatgct gatccgctgc ctacggcaag attcctctgt cgagattgag
      361 tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca ccgcaaagcc
      421 gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgtat gccattgcat
      481 acgattcgca atactctaat ttgtagtaat aggcatccac gaattactga caatagcact
      541 tatgaacaaa ttatggcaga agagttcacc caattaatat ctaaatcagc agcggttgat
      601 gatattcaaa tggaaataga cgagagattt atgaatcgca aaatttcatt tcgtggttcc
      661 tctttgctga caatcattaa tagcattgca gttacagatt tactgggaat agtcccctat
      721 gaactttata actcccaccg tgactttctt aatttaaaag agataaaact agaacatccg
      781 ttaccttcaa taaagct
//
LOCUS       DAZUGK010000517          794 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZUGK010000517 DAZUGK010000000
VERSION     DAZUGK010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.520"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..770
                     /locus_tag="ACHY5W_005098"
     CDS             54..770
                     /locus_tag="ACHY5W_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4449780.1"
                     /translation="MGRKWANIVAKKMAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAVGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          267 a          132 c          169 g          226 t
ORIGIN      
        1 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
       61 gtaaatgggc caatattgtt gctaaaaaaa tggctaaaga cggtgcaacg tctaaaattt
      121 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
      181 taaacacatc tttaaaattc gttattgaac gtgcaaaaca ggcacaagtt ccaaagcacg
      241 ttattgataa agcaattgat aaagctaaag gcggcggaga tgaaacgttc gtgcagggac
      301 gttatgaagg ctttggtcct aatggctcaa tgattatcgc tgagacatta acttcaaatg
      361 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
      421 cggcaggttc tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag
      481 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
      541 aagaagaagg taacattgtt atttatactg aacctactga ccttcataaa ggaatcgcgg
      601 cgctaaaagc agttggaatc actgagttct caacaacaga attagaaatg attgctcaat
      661 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
      721 aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa tgatatttta
      781 aagaaatctg tctt
//
LOCUS       DAZUGK010000518          794 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZUGK010000518 DAZUGK010000000
VERSION     DAZUGK010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.521"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>794)
                     /gene="ehxB"
                     /locus_tag="ACHY5W_005099"
                     /gene_synonym="hlyB"
     CDS             complement(<1..>794)
                     /gene="ehxB"
                     /locus_tag="ACHY5W_005099"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFS4449781.1"
                     /translation="VELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQ
                     ALTSVLDLFFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRN
                     AENQAFLVETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLI
                     QKSVMVISLWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVE
                     RLGDVLNTPVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNI"
BASE COUNT          258 a          182 c          131 g          223 t
ORIGIN      
        1 ttaatattat tcaaaataac attaccgtca gaagaatacc gaaatctgac atttttaaat
       61 tcgatatccc cctgaatttc cggcagtata tttctgcctg actttttctc taccggtgta
      121 tttaatacat caccaaggcg ctcaacagaa ataccaacct gctgaaaatc ctgccaaaga
      181 tgagccagtc tgataacagg agcaatgacc tgacctgcaa gcatattaaa cgcgattaac
      241 tgcccgatac ttatctctcc cgatatgaca agatgtgctc ccatccatag actaattacc
      301 attacacttt tctgtatcag ctgtatcccc tgctgccctg tcattgcaac cagatttacc
      361 ctgaaactgg aagcaacata cccggccagt tgtttatccc atgtagcaat catctgtggt
      421 gatactgcca tggatttaat tgtatttatt gctgttaccg tttcgacaag aaaagcttga
      481 ttttctgcat tcctgagaaa cttatcatca agacgtcgac gtaataaggg tgatataaac
      541 aatgaccata taacataaca aggtagtgac aataatataa ccagtgttaa ttgagggctg
      601 taataccaca tgacacaaaa aaatattaca gaaaaaaata gatcaagaac tgatgtcaac
      661 gcttgtccgg ttaaaaaatt acgaatttgc tccagttccc ttactctggc aacggtctct
      721 ccgaccctgc gattttcaaa atatgaaaca ggcaatgcaa gcaaatgtct gaataactta
      781 gcgctcagtt cgac
//
LOCUS       DAZUGK010000519          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZUGK010000519 DAZUGK010000000
VERSION     DAZUGK010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.522"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>785
                     /locus_tag="ACHY5W_005100"
     CDS             <1..>785
                     /locus_tag="ACHY5W_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4449782.1"
                     /translation="ATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSEKAMRIYEHYGE
                     ALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKVASVVDTLKLII
                     PQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVEGETLETHAPAF
                     WRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKSIMAEIATLLAG
                     EDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKA"
BASE COUNT          151 a          245 c          243 g          146 t
ORIGIN      
        1 gccacccgta aggccattta tgatgccatc cggccaccgg ctgaaagccc gttcgacacc
       61 atgagcgaag cggagttttc cgccatgagt accagcgaaa aggccatgcg tatctatgag
      121 cattacggcg aggcgctcgc ggtcgatgcc aacggccagc ttctgtcccg ctatgaaaat
      181 ggtgtctgga aggtgctgcc gccacaggac tttgcccggg atgtggccgg gctgtttcag
      241 cgtctgcgcg cgccgttctc ctccgggaaa gtggcctccg tggtggacac cctgaagctg
      301 attattccgc agcaggaagc cccctcccgc cgcctgattg gctttcgtaa cggcgtgctc
      361 gacacgcaga acggcacgtt ccacccgcac agtccgtcac actggatgcg caccctgtgc
      421 gatgtggatt tcaccccgcc ggtggaaggg gaaacgctgg aaacccacgc tcccgcgttc
      481 tggcgctggc ttgaccgtgc cgccggtggt cgtgcggaaa aacgcgacgt gattctggcc
      541 gcactgttta tggtgctggc aaaccgctat gactggcagc tctttctgga ggtgaccggt
      601 cccggcggca gcggcaaaag tatcatggcc gaaatagcca ccctgctggc cggggaggat
      661 aacgccacgt cggccaccat tgagacgctg gaatccccgc gtgaacgtgc cgcgttaact
      721 ggcttctcac tgatacgcct gccggaccag gaaaaatgga gcggcgacgg agccggactc
      781 aaggc
//
LOCUS       DAZUGK010000520          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZUGK010000520 DAZUGK010000000
VERSION     DAZUGK010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.523"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>785
                     /locus_tag="ACHY5W_005101"
     CDS             <1..>785
                     /locus_tag="ACHY5W_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4449783.1"
                     /translation="ATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSEKAMRIYEHYGE
                     ALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKVASVVDTLKLII
                     PQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVDGETLETHAPAF
                     WRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKSIMAEIATLLAG
                     EDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKA"
BASE COUNT          148 a          249 c          244 g          144 t
ORIGIN      
        1 gccacccgta aggccattta tgatgccatc cggccaccgg ctgaaagccc gttcgatacc
       61 atgagcgaag cggagttttc cgccatgagt accagcgaaa aggccatgcg tatctatgag
      121 cattacggcg aggcgctcgc ggtcgatgcc aacggccagc ttctgtcccg ctatgaaaat
      181 ggtgtctgga aggtgctgcc gccacaggac tttgcccggg atgtggccgg gctgtttcag
      241 cgtctgcgcg cgccgttctc ctccgggaag gtggcctccg tggtggacac cctgaagctg
      301 attattccgc agcaggaagc cccctcccgc cgcctgattg gctttcgtaa cggcgtgctc
      361 gacacgcaga acggcacgtt ccacccgcac agtccgtcac actggatgcg caccctgtgc
      421 gatgtggatt tcaccccgcc ggtggacggt gaaacgctgg agacccacgc ccccgcgttc
      481 tggcgctggc ttgaccgtgc cgccggtggc cgtgcggaaa aacgcgacgt gattctggcc
      541 gcactgttta tggtgctggc aaaccgctac gactggcagc tctttctgga ggtgaccggt
      601 cccggcggca gcggcaaaag tatcatggcc gaaatagcca ccctgctggc cggggaggat
      661 aacgccacgt cggccaccat cgagacgctg gaatccccgc gtgaacgtgc cgcgttaact
      721 ggcttctcac tgatacgcct gccggaccag gaaaaatgga gcggcgacgg agccggactc
      781 aaggc
//
LOCUS       DAZUGK010000521          776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZUGK010000521 DAZUGK010000000
VERSION     DAZUGK010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.524"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>776
                     /locus_tag="ACHY5W_005102"
     CDS             <1..>776
                     /locus_tag="ACHY5W_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFS4449784.1"
                     /translation="AAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSV
                     WPKSIRGSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWE
                     KDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRS
                     ITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLM
                     DKVVHYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEA"
BASE COUNT          180 a          194 c          236 g          166 t
ORIGIN      
        1 gctgccgcca aaaactaccg tgaaaaatcc gtggacgtgg tctgctatga cgaactttcc
       61 tcgttcgagc cggatgtcga aaaagagggc tcgccaaccc tgctggggga taagcgtatt
      121 gaggggtcgg tgtggccaaa atccattcgc ggctcgacgc ctaaaatcaa aggcacctgc
      181 cagatcgaaa aagccgctaa cgagtcggcg cattttatgc gtttttatgt gccctgcccg
      241 cactgtgggg aggcgcagta tctgaaattt ggcgatgagt ccacgccttt tgggcttaaa
      301 tgggagaagg acagccccga aagtgttttc tacctctgtg aacatcatgg ctgcgtgatc
      361 catcagtctg aactggacca gagcaacggg cggtggatct gtgaaaacac gggcatgtgg
      421 acccgtgacg gtctgacgtt tttcagcgcc cggggtgatg aaattccgcc gccgcgctcc
      481 atcacgttcc atatctggac ggcgtacagt ccgtttacca cctgggtaca gatagtctat
      541 gactggctgg atgcactgaa agatcccaac ggcctgaaaa cctttgtgaa caccacgctg
      601 ggcgagacct gggaagaagc cgtgggcgaa aaactcgatc accaggtact gatggataag
      661 gtggtgcatt acacggcggc ggtacctgcc cgggtggttt atctgacggc gggcattgac
      721 tcgcagcgaa accgttttga gatgtatgtc tggggatggg caccgggaga ggaagc
//
LOCUS       DAZUGK010000522          769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZUGK010000522 DAZUGK010000000
VERSION     DAZUGK010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.525"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHY5W_005103"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHY5W_005103"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS4449785.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          203 a          197 c          186 g          183 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta atgtttttgt cgtgacataa gaataattc
//
LOCUS       DAZUGK010000523          763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZUGK010000523 DAZUGK010000000
VERSION     DAZUGK010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.527"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..713
                     /gene="slp"
                     /locus_tag="ACHY5W_005104"
     CDS             147..713
                     /gene="slp"
                     /locus_tag="ACHY5W_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFS4449786.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          234 a          162 c          165 g          202 t
ORIGIN      
        1 ccaaaatcaa tggaattgaa ctaaacaaaa gggatataaa catcagacag gtttacgtta
       61 ctatcaggca tatcgcctca gaatcagatg aaaactataa agaaatatct attatggttt
      121 taatatttgt tgataaggat agtaacatga acatgacaaa aggcgcactc atcctcagcc
      181 tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa tatcaaaggc aataaccaac
      241 ctgatattca aaaaagtttt gttgctgttc ataaccagcc ggggttatat gttggtcaac
      301 aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg caaaacggat acgttgttag
      361 aaatcgctgt attaccgttg gatagctatg cgaaacctga tattgaagcc aactatcagg
      421 gccgactgct cgccagacaa agcggcttcc ttgacccagt gaactatcgt aatcactttg
      481 ttaccatcct cggtaccatt cagggtgaac aaccaggctt tatcaataaa gtcccgtata
      541 acttcctgga agtgaatatg cagggcatcc aggtgtggca tttgagagaa gtggttaata
      601 ccacctataa cctgtgggat tacggctatg gtgcattctg gccggaaccg ggctggggtg
      661 cgccttacta caccaatgcg gtgagtcagg taacacctga gctggtcaaa taacaccacc
      721 gaaagatgca gatgctttct cagcatctgc atcatgtatt aca
//
LOCUS       DAZUGK010000524          763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZUGK010000524 DAZUGK010000000
VERSION     DAZUGK010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.528"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          251 a          162 c          143 g          207 t
ORIGIN      
        1 ctgaaccgtt tcactatact ctacttaatt cgagttacag gaaggcggca agcgagtgaa
       61 tccccaggag cttacataag taagtgattg gggtaagcga acgcagtcgc agcacatgca
      121 acttgaagta tgacgagtat atatatctat tagctgtttc ccggacataa gctacagcca
      181 gcactaacct ggaacgggtg cattataaaa aggaacaagg gacaaatagc tgaacagtaa
      241 ccgcgttcgt ctcttcccgc ataaccgtac cattcgtatt aagaagtatt gcccatatcc
      301 attcggcgaa agaggatctg ccatacatca agtccgtttt acttggcatc agatcatcgc
      361 cgtcattaac tctgttaaag tcggacaaac gattagcaag atctgtgatt caaaagtgct
      421 tagcgctaag gtgggactgg ttacagattc attaaccctg ttctttgtcg cacctatcga
      481 tcggtgtaat gccacaacag gtttatgtta atcagaacaa tgcgcccctc tataaagaga
      541 attctttgat aaggcaactt gaaaagggaa agctaactga tatcaatgta ccactttagt
      601 aatcatcaca ttatatacag gccaaaataa gtttcctgaa agccagtatc tctaaatcaa
      661 tcattaatga tatattttat cgtcacaata aaaattatct aaattctatt acttatgtaa
      721 ataagcacaa ccttttcata gacacaaaaa ctatggctga cgc
//
LOCUS       DAZUGK010000525          763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZUGK010000525 DAZUGK010000000
VERSION     DAZUGK010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.529"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            113..394
                     /locus_tag="ACHY5W_005105"
     CDS             113..394
                     /locus_tag="ACHY5W_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449787.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSMTADNYVRVVVEVMR
                     GDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT          204 a          150 c          195 g          214 t
ORIGIN      
        1 tgatgcaaaa aagatgatga ttgtggttaa tttttgttcg acatctttta gcgtgacaga
       61 ttctttacaa aaaatctgag cttgttttct tcaccagcgc gatgggggca caatgacaga
      121 agccgaaata ctgggattaa tccgccgcgt cgccggaatc agccagcagg ctgacgaaca
      181 ggccacgcag ccggacagca tgaccgcaga taattatgtg cgtgtagtgg tggaggtgat
      241 gcgcggtgat ggtatccagc ttaatgatgt ggatatgcgc aatatacgaa ccagagtcct
      301 tgagttgctg gcataccgtc gccgttctca acaacggagg gagagcgcga aaaatactta
      361 ccagtggaag acgccggaac ggctgcggag gtaacttgtt gatattcccg ataacgcaaa
      421 attgcgttgg ctggtgggtg agttgcagat ctgcaactcg atcatgaaat tacggaaact
      481 acccgtagtt tgggtagtaa gagcaacacc cagattttgg ggcttacttg cgatacccaa
      541 ataaagggta tcggtggaag aaatatcgtt tctcatatgt gagttccgag agcggaattc
      601 cgcctctgat ttgtcattgt gatcatgcat agcggcaata attaatattg cttccttttt
      661 tgctttttta tcgcttaagc aggtactaaa tctgggacta tgttgtaggg ggcataactc
      721 aatagtacct tataatcatt tagttacctt ttatttactt ctt
//
LOCUS       DAZUGK010000526          762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZUGK010000526 DAZUGK010000000
VERSION     DAZUGK010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.530"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(20..>762)
                     /gene="eae"
                     /locus_tag="ACHY5W_005106"
     CDS             complement(20..>762)
                     /gene="eae"
                     /locus_tag="ACHY5W_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFS4449788.1"
                     /translation="QPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSTGK
                     ATVSATVSDGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGG
                     DGTYSWYSENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQ
                     AYYADAMSICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQSSGV
                     SSTYNLITQNPLPGVNVNTPNVYAVCVE"
BASE COUNT          209 a          164 c          143 g          246 t
ORIGIN      
        1 agccggggtg gttatggaat tattctacac aaaccgcata gacatttgga gtattaacat
       61 taaccccagg aagagggttt tgtgttatta ggttataagt gcttgatact ccagaactct
      121 gctcactaga tgtctgttta atccaagcag ttattgagtt catagaacta taatggctat
      181 atttatttgc agccccccat gagtcataaa tatctgacaa taccgtctgt gtggatggta
      241 ataaattttt gcaaatggac atagcatcag cataataggc ttgcttatcc acttttatca
      301 tatacgacgg tgcttttata gtgtaactta ctgtttgctt atcaccagat gtggctttaa
      361 ttacgacact gcctttacca ttcaaagtga ctttccctga tgcatcgaca gtcgcgatac
      421 tggtattttc tgaataccat gaatatgtac catcaccacc gcttgctttc agtttaaact
      481 gaccatattg cagccaaata ttaggcaact cgcctctgac attgttacca ataatatcaa
      541 ccttgttgtc aattttcagt tcatcaaaaa aagtgacctc agtcgcttta acctcagccc
      601 catcactgac tgtcgcacta acagtcgctt taccggtgga actggaagtt agtgttatcg
      661 tcgcacgacc atcatttccc gtggttgctt gcgtttgaga cttaccgttg aacatcccaa
      721 agtttgttga gaatgtaacg gattgattat taactggctg ac
//
LOCUS       DAZUGK010000527          762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZUGK010000527 DAZUGK010000000
VERSION     DAZUGK010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.531"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>762
                     /locus_tag="ACHY5W_005107"
     CDS             <1..>762
                     /locus_tag="ACHY5W_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS4449789.1"
                     /translation="ILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDL
                     IGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCV
                     GGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTN
                     KPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHAGRV
                     ALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLAS"
BASE COUNT          181 a          187 c          235 g          159 t
ORIGIN      
        1 gcattctgct ggttccgtgc tgtcgtggtg gttcagcgtt caccaccgga gccgatggca
       61 catacagtga cgcgagtggt gcctcggaga attcaacccg ctggggtgtg gacaagccgc
      121 tgtataagga ccttatcggt cgaacaaaag cagcactgaa gaagaatccg aaaaatgtgc
      181 tgtttgccgt ggtgtggatg cagggggaat ttgattttgg cggtacgccg gcaaatcacg
      241 cagcacagtt tggtgcgctg gttgataaat tccgtgcaga cctggcggat atggcaggtc
      301 agtgcgtcgg tggctctgct ggcggtgttc cctggatatg tggagatacg acgtatttct
      361 ggaagcagaa gaacgaatcc acgtaccaga cggtgtacgg cagctataaa aacaaaacgg
      421 aaaagaatat ccatttcgta ccgttcatga ccgatgagaa cggggtgaat gtgccgacga
      481 acaaaccgga agaagacccg gacattccgg gtatcggata ttacggttcg aaatggcgtg
      541 acagctcagc cacctggacg tcacaggaca gggcgagcca tttcagcgcc tgggcacgcc
      601 gtgggattat ttccgaccgt ctggcaacgg cgattttgcg ccatgcggga agagtggcgc
      661 taaacgcggg ggcatcatcg acagtatcag aggtgcgccc ttcatcgcct tccggtgcag
      721 aagccacagg cgtcacaaca ctgctctctt accttgccag cg
//
LOCUS       DAZUGK010000528          755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZUGK010000528 DAZUGK010000000
VERSION     DAZUGK010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.532"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHY5W_005108"
     CDS             <1..375
                     /locus_tag="ACHY5W_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4449790.1"
                     /translation="FPRGTYATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTDER
                     LFSVVIRQSSGQCTEKTFSLPVMLYRGVFRTGEIYHPGDTVTWGGSLWHCNSMTGDKP
                     GEAHSSGWTLAAKRGRDAGGGK"
     gene            372..698
                     /locus_tag="ACHY5W_005109"
     CDS             372..698
                     /locus_tag="ACHY5W_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4449791.1"
                     /translation="MTALLTLEEIKAHLRIDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT          168 a          170 c          255 g          162 t
ORIGIN      
        1 tttccccggg gcacgtatgc cacacacctg ggtggactct ggcgggcgta tgaaaaaacg
       61 cacgggatgc ggggatggga atgcctggtt gacggggtgg cggatatcga cgtcagcatg
      121 acggatgagc ggttgttctc tgtggtgatc cggcagagca gtggccagtg tacggaaaaa
      181 acattttccc tgccggtgat gctctaccgt ggtgtgttca gaaccggcga aatttaccac
      241 cccggcgata cggtgacgtg ggggggctcg ttgtggcact gcaacagcat gaccggtgat
      301 aagcccggtg aagcccattc atcaggctgg accctggctg ccaaacgtgg acgggatgca
      361 ggaggtggaa agtgacagca ttactgacac tggaagaaat caaggcacat ctgcgtatcg
      421 accatgacgc ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtactgc
      481 tggcctacat tcagggcagc cgggataaag tgattcgtga ggacggtgag ctgatcccgg
      541 gtgaggcatt aacccggatg aagggggctg ccatgcgcct gaccgggatg ctgtacagga
      601 atccggatct tgcggagcgg gaagagctga ttcaggggga actccccttt tctgtatctg
      661 ttctgattta cgatttgcgt tgtccgacgg tgttatgagg agggaggaat ggcaatatct
      721 gcaggtcgtc tgacacagat gataagtgtt ctgaa
//
LOCUS       DAZUGK010000529          750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZUGK010000529 DAZUGK010000000
VERSION     DAZUGK010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.533"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..749)
                     /locus_tag="ACHY5W_005110"
     CDS             complement(<1..749)
                     /locus_tag="ACHY5W_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS4449792.1"
                     /translation="MVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFVAT
                     IVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQSTPY
                     TMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKP
                     EIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYS
                     GGALLVLFATLSGVVYGLSWMWSGRLWVATL"
BASE COUNT          308 a          173 c          117 g          152 t
ORIGIN      
        1 aaaagggtgg caacccacaa acgcccactc cacatccatg acaatccata cacaacacca
       61 gataacgtgg caaataatac aagtaaagca ccacctgaat agtgataaaa accaaacaac
      121 aaagccgcca caattaatgc aaccaatgga gacgttactt ctgatagccg tgattgaata
      181 taccctctaa ataatgattc ctctgccaga gacacaaaaa acaaattagc caatataaac
      241 tctggcaacc actcaggaaa atgaatctct ggctttaatc caccaaaaaa aacagccaaa
      301 aacaggataa gaggaacaga gagcgacaga gccccccact tccacaaaga cacttctgat
      361 tttacttctt ttttaaacaa agaagatgta cacaggacta acaaaaatgg caccagcgct
      421 ttatcaaaat taaaatacat tgtataggga gtactttgag ggccaacagt aacagaattt
      481 agcactacag gattgtgaaa ccctggccat agatggaaag ataatgctat ggctgataaa
      541 actatgccaa cttcatatat agatttagcc caggcgttat atttccagtt gaacttcaaa
      601 ataataaaaa aaacgattgt agcaacaaaa aataacaccg accaatcaat aatatcatta
      661 agcacagcca ggacaacaga caccgtcaac aatgaaaaag ccactacctt atgccaactg
      721 agagttgaca gtgacagtac taacacaatc
//
LOCUS       DAZUGK010000530          746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZUGK010000530 DAZUGK010000000
VERSION     DAZUGK010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.534"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..577
                     /gene="escU"
                     /locus_tag="ACHY5W_005111"
     CDS             <1..577
                     /gene="escU"
                     /locus_tag="ACHY5W_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFS4449793.1"
                     /translation="FMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFS
                     VFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKS
                     TVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLART
                     LYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
     gene            complement(574..>746)
                     /gene="etgA"
                     /locus_tag="ACHY5W_005112"
     CDS             complement(574..>746)
                     /gene="etgA"
                     /locus_tag="ACHY5W_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFS4449794.1"
                     /translation="YILASNIKSRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKT
                     YNKLKNNEQIID"
BASE COUNT          230 a          128 c          139 g          249 t
ORIGIN      
        1 ttttatgggg cattcatatg caaatgagtt tgctaatttc acaggactga acgcatatca
       61 agctcttgtc gttgttgcct tttttgtttt tcttttatgg aaaggcgtgc tattcggata
      121 tctactcttt tcagtatttg atttctggtt ccagaagcat gagggactga agaaaatgaa
      181 aatgagtaaa gatgaggtga aacgagaagc caaggatact gatggtaacc ctgaaattaa
      241 aggggagcgc cgtcgccttc attccgagat acaaagtgga agtttggcta ataacatcaa
      301 aaaatcaacc gttattgtta aaaacccgac tcacattgcg atttgcctat actataaact
      361 tggggagact ccattacctt tagttattga aacaggaaaa gatgccaaag ctctacagat
      421 cattaaactg gctgaactct atgatattcc agtgattgaa gatattcctt tagcaagaac
      481 tctctataag aatatacata aaggacaata tataacagaa gacttttttg aacctgtggc
      541 acaattgatt cgtattgcga tagaccttga ttattaatcg ataatttgct cattattctt
      601 taatttatta tatgttttat atattttcat tgcgtattgc cttctgagtt ctactgcatt
      661 tggtgtatta aagtaacctg cattatacgc accaactgca tcccatgtat tccctctgga
      721 ttttatgtta gaggctaaga tataac
//
LOCUS       DAZUGK010000531          745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZUGK010000531 DAZUGK010000000
VERSION     DAZUGK010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.535"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(258..>745)
                     /locus_tag="ACHY5W_005113"
     CDS             complement(258..>745)
                     /locus_tag="ACHY5W_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFS4449795.1"
                     /translation="KERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHV
                     DMIMGSHVLKDMPLRKQAEILFCLGGGFCKYSSSDMFGTEYDSPEILRRYANGLIEQA
                     YKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAW
                     R"
BASE COUNT          256 a          172 c           98 g          219 t
ORIGIN      
        1 aatcatatgc cctaatcaac atcctcaagc actacgatat atgcagtatc ctattcatat
       61 attaccacca tatcagacaa atcgaatcac aacgagttct atcgtactct ttcatcttcg
      121 acactgtaaa ttttaagaac attcggctca tgccactaaa ccaagacata ataacaacga
      181 agcaataaat gttcccactg aacatgtgca aacctaaaaa gacagtgtta caataatatt
      241 catccatggt ctctaaatca acgccacgca acaggataat acaatgaaaa tatttcagga
      301 aaagattctt ttgcatgctc cgttagcata tcagtcagca cagcggtaca agtaaaaaca
      361 ttatttcttc cttgtagcct gtctaaaata tcattgtaat aataaactga gccaaatacc
      421 tgaggatctg ttttataagc ttgttcaatc aatccatttg catatctccg tagaatctca
      481 ggagaatcat actctgtacc aaacatatca ctcgatgagt atttacagaa acccccccct
      541 aaacaaaata atatttcagc ctgttttctt agtggcatat ctttcaatac atggctcccc
      601 ataatcatat ctacatgctg agcgttcagt tcccaaccat caagaaaggg attccagaca
      661 caggcaagtt tcctttgctg atcatcagca atcattttat aatctgattt attggaatta
      721 agtgcctcaa taaatctctc tttga
//
LOCUS       DAZUGK010000532          745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZUGK010000532 DAZUGK010000000
VERSION     DAZUGK010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.536"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..602
                     /gene="fimA"
                     /locus_tag="ACHY5W_005114"
     CDS             39..602
                     /gene="fimA"
                     /locus_tag="ACHY5W_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4449796.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSNNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          193 a          148 c          182 g          222 t
ORIGIN      
        1 tctttattca gctacttaaa tataaatttt ggagaattat gaaactcaaa catgttggta
       61 tgattgtcgt ttctgtgttg gcgatgtcgt ctgctgcggt aagcgcagcc gagggtgatg
      121 aatcagtaac gaccactgtt aatggcggtg ttattcattt taaaggtgaa gtggtaaatg
      181 ccgcttgtgc gattgattcc gaatcaatga accaaacggt tgagctgggt caggttcgtt
      241 cttctcgcct ggctaaagcg ggtgacctca gctccgccgt tggcttcaat atcaagctga
      301 atgattgtga taccaatgtt tccaataatg cagctgttgc atttctgggt actactgtca
      361 ccagtaatga cgatacgtta gcgctgcaga gttcagcggc aggctctgcc caaaatgtcg
      421 gtatccaaat tttggaccgt acgggtgagg tattaatact tgatggtgcc acttttagcg
      481 ctaaaactga cttgattgat ggcacgaata tactgccgtt ccaggctcgt tatattgcac
      541 tcgggcagtc cgtagctggt actgcaaacg cagatgcgac cttcaaagtt caatatctat
      601 aattcaatct taaggtggct atacgtcatc tgaaattgaa acagggtgtg tgtggcatgg
      661 ctacacacac ccgagcaatt caccaatgtg aatctcatta ttaatgagga gtggtttgta
      721 tttggagcgc tatgaaaaat cacat
//
LOCUS       DAZUGK010000533          743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZUGK010000533 DAZUGK010000000
VERSION     DAZUGK010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.537"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..339
                     /gene="kil"
                     /locus_tag="ACHY5W_005115"
     CDS             118..339
                     /gene="kil"
                     /locus_tag="ACHY5W_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4449797.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIRVFLGRDGLPVKAESW"
     gene            339..509
                     /locus_tag="ACHY5W_005116"
     CDS             339..509
                     /locus_tag="ACHY5W_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFS4449798.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            584..>743
                     /locus_tag="ACHY5W_005117"
     CDS             584..>743
                     /locus_tag="ACHY5W_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449799.1"
                     /translation="MITSYEATVVTTDDIVHEVSLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKT"
BASE COUNT          252 a          142 c          153 g          196 t
ORIGIN      
        1 aaaaacagtg ttatgggtgg attctctgta tccggcgtta attgttaact ggttaacgtc
       61 acctggaggc accaggcacc gcatcgacaa aattcatttg taaaaatgga gataattatg
      121 attgctcatc acttcggaac tgatgaaata ccacgtcagt gtgtgacccc tggcgattat
      181 gttcttcatg aaggtcggac atatatcgcc tcggcaaaca atattaaaaa gcgaaaactt
      241 tatattcgta gcctgactac aaaaacatgc atttctgact gcatgattag agtcttcctc
      301 ggtcgtgatg gtttacctgt aaaggcggag tcatggtaat gactaagaaa ataaaatgtg
      361 cttatcacct ttgcaataaa gaaattgaag aaagcaaaat cattacaaga ccacttcatt
      421 tcatgcgtgg agttatacca acgacggaaa tgaaaaaata ttgtagtgaa atctgtgccg
      481 aaaaagacca gatggcacac gaactttaat taactgacta tccgaaactg aatttatgcc
      541 agcaatggca gggattcgct caaccttaat taaggagaaa aacatgatta ccagttatga
      601 agccactgtt gttactactg atgacattgt tcacgaagtc agcctggaag gaaagcgtat
      661 tggctacgtg attaaaacag aaaataaaga aacccctttc actgtggttg atatcgacgg
      721 tccatcaggc aacgttaaaa cac
//
LOCUS       DAZUGK010000534          736 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZUGK010000534 DAZUGK010000000
VERSION     DAZUGK010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.538"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            186..635
                     /gene="glmS"
                     /locus_tag="ACHY5W_005118"
     CDS             186..635
                     /gene="glmS"
                     /locus_tag="ACHY5W_005118"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFS4449800.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            635..>736
                     /locus_tag="ACHY5W_005119"
     CDS             635..>736
                     /locus_tag="ACHY5W_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449801.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTP"
BASE COUNT          201 a          165 c          178 g          192 t
ORIGIN      
        1 ttaacatgga aatttaaaaa cacactttaa acaatcattt cccgaaagac attttgaaaa
       61 tagatcaatt gcaacattac ggtcagataa agacactcct gtgatattaa acctgtgccg
      121 ttatgaacgg ccataaatac gacgtgtgta acttatttta ctctctgtct tacaaaggat
      181 tacccatgaa gaaagcaaca cttgtgattg gcgttattgg cgcggactgc catgcagtag
      241 gcaataaagt tctggaccgc gtttttagca accacgactt cagagtcatt aatctgggcg
      301 taatggtcag ccaggacgaa tatattgatg cggccattga aactggcgct gacgcgattg
      361 tcgtctcctc tatctatggg catggcgata tcgactgtct gggcatgcgc gaacgctgca
      421 tcgagcgtgg actgggggac atcctgctct atgtgggcgg taacctggtg gtcggaaaac
      481 atgacttcgc tgacgtggaa accaaattca aagagatggg atttgatcgc gtctttgccc
      541 caagtcatga cttagaagat gtttgtcagc tgatggccca cgatatcaac caacgtcatg
      601 acgttgatac tcgtattctt gaagaggcta tctgatgcaa atcgtctctg tcgatattgg
      661 atcgacgtgg accaaagcag ccctcttcac ccgggagggg gacgcgttaa cgttggttaa
      721 ccacgtactg acccca
//
LOCUS       DAZUGK010000535          731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZUGK010000535 DAZUGK010000000
VERSION     DAZUGK010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.539"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            183..>731
                     /gene="pagP"
                     /locus_tag="ACHY5W_005120"
     CDS             183..>731
                     /gene="pagP"
                     /locus_tag="ACHY5W_005120"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFS4449802.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTERYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMR"
BASE COUNT          199 a          145 c          176 g          211 t
ORIGIN      
        1 attcccatcg cagaaaacga cgaatcatct ttaatcgatg cgcggaaata tttaacttga
       61 acaagcggaa ataaatagag cagctattca gattattctt tatgttgggg ctattaaggt
      121 tatgttaatt gtagctttgc tatgctagta gtagattttt gataaatgtt ttatggtcac
      181 aaatgaacgt gagtaaatat gtcgctatct tttcctttgt ttttattcag ttaatcagcg
      241 ttggtaaagt ttttgctaac gcagatgagt ggatgacaac gtttagagaa aatattgcac
      301 aaacctggca acagcctgaa cattatgatt tatatattcc tgccatcacc tggcatgcac
      361 gtttcgctta cgacaaagaa aaaaccgaac gctataacga gcgaccgtgg ggtggcggtt
      421 ttggcctgtc gcgttgggat gaaaaaggaa actggcatgg cctgtatgcc atggcattta
      481 aggactcgtg gaacaaatgg gaaccgattg ccggatacgg atgggaaagt acctggcgac
      541 cgctggcgga tgaaaatttt catttaggcc tgggattcac cgctggcgta acggcacgcg
      601 ataactggaa ttacatccct ctcccggttc tactgccatt ggcctccgtg ggttatggtc
      661 cagtgacttt tcagatgacc tacattccgg gtacctacaa caatggcaat gtgtactttg
      721 cctggatgcg c
//
LOCUS       DAZUGK010000536          731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZUGK010000536 DAZUGK010000000
VERSION     DAZUGK010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.540"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..402)
                     /locus_tag="ACHY5W_005121"
     CDS             complement(<1..402)
                     /locus_tag="ACHY5W_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFS4449803.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPV"
     gene            complement(508..711)
                     /locus_tag="ACHY5W_005122"
     CDS             complement(508..711)
                     /locus_tag="ACHY5W_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFS4449804.1"
                     /translation="MQSLTAQLRLGPADILESDENGIIPEQDRVITQVVILDTDKKLI
                     QCVVRPLQILRADGTWENIGGMK"
BASE COUNT          196 a          203 c          134 g          198 t
ORIGIN      
        1 cacaggcaag ctgttatagt ccacactcgg taactctggc ggtgcataat caacaggagg
       61 taaatcaggt ctattcgaat gaacagcacc tctttccata gccctattca ctggcgatga
      121 attcagcatc acctcaattt tcctgctaat ctctccctta ggccatccca gcctgtgcaa
      181 cagattagta aagcaaccac tatgactttc tcttgtaacg caaaacttat tatcagtgac
      241 aacaacacga tatgttctgt tgcccacctt tacttgcgcc ccattatccg agctagcagc
      301 tgcatccctt acagaggata aaatacgact gtcaggagaa gtcaggtttc tggttaaaga
      361 attccatgaa caccccaaat ttacagaaga tggtgatagc atacatttca accttcaaaa
      421 tgaatcaatc tttactttct taacaaacat caccatgaca tgacaacaaa aaccggagcc
      481 ggactccggt tttgtgaagc tgtcgggtta cttcatcccg ccaatatttt cccacgtccc
      541 gtcagcacgc aggatttgca gcggtcttac cacacactgt atcagctttt tatctgtatc
      601 cagtatcacc acctgtgtga ttaccctgtc ctgctccgga ataatgccat tctcatctga
      661 ctccaggatg tctgccggcc ccagtcgcag ttgtgctgta agcgactgca cgtgttcacg
      721 gccatcatgc t
//
LOCUS       DAZUGK010000537          727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZUGK010000537 DAZUGK010000000
VERSION     DAZUGK010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.541"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>727
                     /locus_tag="ACHY5W_005123"
     CDS             <1..>727
                     /locus_tag="ACHY5W_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFS4449805.1"
                     /translation="LIARGWGVSLVSRCLRVSRAQLYVILRRTDDWMDGRRSRHTDDT
                     DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPT
                     VPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGG
                     FNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAV
                     RSPESNGIAENFVKTIKRDYISI"
BASE COUNT          167 a          176 c          226 g          158 t
ORIGIN      
        1 cccttattgc ccggggatgg ggagtaagct tagtcagccg ttgtctccgg gtgtcgcgtg
       61 cgcagttgta cgtcattctc agacgaaccg atgactggat ggatggccgc cgcagtcgtc
      121 acactgatga tacggatgtg cttctccgta tacaccatgt tatcggagag ctgcccacgt
      181 atggttatcg tcgggtatgg gcgctgcttc gcagacaggc agaacttgat ggtatgcctg
      241 cgatcaatgc caaacgtgtt taccggatca tgcgccagaa tgcgctgttg cttgagcgaa
      301 aacctactgt accgccatcg aaacgggcac atacaggcag agtggccgtg aaagaaagca
      361 atcagcgatg gtgctctgac gggttcgagt tctgctgtga taacggagag agactgcgtg
      421 tcacgttcgc gctggactgc tgtgatcgtg aggcactgca ctgggcggtg actaccggcg
      481 gcttcaacag tgaaacagta caggacgtca tgctgggagc ggtggaacgc cgcttcggca
      541 acgatcttcc gtcgtctcca gtggagtggc tgacggataa tggttcatgc taccgggcta
      601 atgaaacacg ccagttcgcc cggatgttgg gacttgaacc gaagaacacg gcggtgcgga
      661 gtccggagag taacggaata gcagagaact tcgtgaaaac gataaagcgt gactacatca
      721 gtatcat
//
LOCUS       DAZUGK010000538          726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZUGK010000538 DAZUGK010000000
VERSION     DAZUGK010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.542"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..139
                     /locus_tag="ACHY5W_005124"
                     /pseudo
     CDS             1..139
                     /locus_tag="ACHY5W_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            298..>726
                     /locus_tag="ACHY5W_005125"
     CDS             298..>726
                     /locus_tag="ACHY5W_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFS4449806.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGK"
BASE COUNT          201 a          127 c          194 g          204 t
ORIGIN      
        1 cgggctgatt gcccctgagc agaaacgcga actgaataac caggggatct ggcttcgtga
       61 aggtgaacgg gcggcggcgg acggcagtat caccggaacg ccacgaaact cccggattgc
      121 gtcattttgg atggagggta catttcttac ctgtttgtat gttctggtgt cgattcatag
      181 tcttttcaat gagttgtgat tttatgagtt tcctctcttt gcttgataat gagttagttt
      241 atcgcttgtt attggcttga atggactaca tgacggacta aaaaatgagg gcgatagatg
      301 tcggtaaagc cattaaccgt gactgaagtt aaggggatga aaccacgtga aaaggactat
      361 gccgtttatg atgggttcgg tttattgctg aatgtgagta aagccggtgg gaaagtgtgg
      421 cgtttccgtt atagccatcc gataacgaag aaacggcaga catacacgat aggacgtttt
      481 cctgaattct cactcgcgga agcacgggaa gtacgtgatg aacttcggcg aatgattgca
      541 cgtggagttg atccagtgac ggagaagaaa aatcgtaaaa ttgagatgtc actaaaaaat
      601 ctacagacat ttgaagctgt tgctaatgca tggttcgctt ttaaaaaggg atctgaattg
      661 cggaaaccta cgctgtataa tatcgaatat gaagtataca aatatcttgt tcctttcttt
      721 ggtaag
//
LOCUS       DAZUGK010000539          719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZUGK010000539 DAZUGK010000000
VERSION     DAZUGK010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.543"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(160..>719)
                     /gene="escD"
                     /locus_tag="ACHY5W_005126"
     CDS             complement(160..>719)
                     /gene="escD"
                     /locus_tag="ACHY5W_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFS4449807.1"
                     /translation="TEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGV
                     AGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINA
                     MNKNSNAKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYV
                     IDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT          203 a          146 c          134 g          236 t
ORIGIN      
        1 tctgcttaat aggtctcttt caaagagaca cattcacgtt ttctcatgta ggttgatcca
       61 accacgctga taccaaagct gaatcacatc aagcaacaac tatgctcaca acatccacac
      121 aataaaaaac cctccgaaga gggggaagag gataagaaat taatacgaca gtggaatatg
      181 tatatattcg tctgggcggg agatgttgat accatcttta ggatcaatcg cgtctatgac
      241 atagcctcct ttcagaatgc taccaatccc caggctggtg ccattatcaa gagcaattgt
      301 tggattcttc atcgtaccgc tgattctgag tatttttccg gggaaaatat ctgctgaaat
      361 gtacggctga atgttctgaa agagtatctt ggcgttacta tttttgttca ttgcatttat
      421 aatggcaagg atcttactct cattctgttg agaaacttcc cccgcaacaa tgatgacatc
      481 attctttttg aaaatattga catagtttat tagtttattc tttttaaact ctgatgccag
      541 ttcattgatt ttattgttac tgttgttgtg tatttcccat cctgcaactc ctggcgtatt
      601 cagtaacaga ttttcaacct cgctccattt gggtgaaggg ggaatgtatc ccgataacag
      661 aaagaatcca ggcttattac cttttgtaat aatgatatct ttgtaaccgt attccgtta
//
LOCUS       DAZUGK010000540          717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZUGK010000540 DAZUGK010000000
VERSION     DAZUGK010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.544"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>717)
                     /locus_tag="ACHY5W_005127"
     CDS             complement(<1..>717)
                     /locus_tag="ACHY5W_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449808.1"
                     /translation="ESESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAE
                     QYANWKFEAEEHEKRLATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVK
                     PELSAVLLDVDLAEIEDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLV
                     GIVLHTLPFDNVIVSGFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVE
                     ALGDQPVIRKMSSTFIFQPI"
BASE COUNT          159 a          197 c          173 g          188 t
ORIGIN      
        1 tcaataggct gaaaaatgaa ggtactgctc atctttcgaa taaccggttg atcgcctaac
       61 gcttcaaccg gatcaatgtg ttttatgcct gcaaaattta ctgacgacat ctggctgcga
      121 gtgcatttgc aggacaggat atactcatcc tccagatagc cggtccgctt actgacccgt
      181 tgcgtaaagc ctgacacaat cacgttgtcg aaaggtagcg tatgtaaaac gataccgacc
      241 aggcggaaca agcagccatg gacgtggtgg gcatagtttt cgcgtacggc tttttgcgtc
      301 atggcttttt ccgtcagctc cgttccccgg gcattaacgc cgtaaatctt atcagggaaa
      361 tcttcaatct cagctaaatc aacgtccagc aggactgctg atagctccgg ctttacttca
      421 aatgcaacaa gcgtttcacg cggccattca gtttctgcca gcacacccgc caggtatgat
      481 tcgaaaaacg cggcgtctgt ccggaactgc tgccgggcat ctgcctgagc cgtggcgagc
      541 cgtttttcat gttcttcagc ttcaaacttc cagtttgcat actgttcggc ccaggccgtc
      601 cgttgttgct ggtaccggtg ctgtcgaagg gtattctcac gctccacatc aatcagctcc
      661 tgctgccacc ggcgaagatt ttcggcgtgg cgctcagctt tagctgattc cgattca
//
LOCUS       DAZUGK010000541          699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZUGK010000541 DAZUGK010000000
VERSION     DAZUGK010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.545"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(312..>699)
                     /locus_tag="ACHY5W_005128"
     CDS             complement(312..>699)
                     /locus_tag="ACHY5W_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449809.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZUGK010000542          698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZUGK010000542 DAZUGK010000000
VERSION     DAZUGK010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.546"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>698
                     /locus_tag="ACHY5W_005129"
     CDS             <1..>698
                     /locus_tag="ACHY5W_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFS4449810.1"
                     /translation="INTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDAV
                     IAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFRQ
                     DTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGTG
                     KYDWLNPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSKLKTENLPELFLTKIDTD
                     TFFSQWSTKTKKDL"
BASE COUNT          240 a          124 c          147 g          187 t
ORIGIN      
        1 atcaatacaa ggattcctcg agacacaatg cccatccttc gtcgttatca cctatggcct
       61 gatggatgga atattcgtgg gctcaatgga ttcatgctat cacataaagg tagtgaagtg
      121 attgatgctg ttatcgcagg ccagaatcag gcttacaggg aactaagaag aataagagat
      181 aatattcata gtgaaatata cttcaaacaa actgatgaat tgtcctcact tccagataca
      241 gacaaaattg gagggattct ggtaaaaaaa tacctttcag gaagtctttt ttcaaaattc
      301 agacaagata ctattatccc ggaggcattg agcaccctcc agatatcagg tcctgacttg
      361 attcaaagaa agatgttgca atttttcagg agtagagggg tgttaggtga agagttcatt
      421 aatgaaagaa aactgagtga taaagcttat attggtgtct acaaaacaac tggcacaggg
      481 aaatatgact ggttaaaccc tgaatcgatc ggcgttaatg atgtcacgcc cgcagatgaa
      541 agtacctggt gtataggaaa aggccggtgt gttgatgact tcctgttcaa agatgtttca
      601 aaactaaaaa cagaaaatct tccagaatta ttcttaacaa aaatagatac tgatacgttt
      661 ttctctcagt ggtcaaccaa aaccaagaaa gatctgca
//
LOCUS       DAZUGK010000543          696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZUGK010000543 DAZUGK010000000
VERSION     DAZUGK010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.547"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>696
                     /locus_tag="ACHY5W_005130"
     CDS             <1..>696
                     /locus_tag="ACHY5W_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFS4449811.1"
                     /translation="EKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPGTGKLLRREQ
                     DYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVVSSLHFIAEHAKYTLLSWPEFY
                     RHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTEDTAHYSILQENLM
                     TVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLHKTAWNQERI
                     TLLFNEKGVKRAL"
BASE COUNT          222 a          146 c          129 g          199 t
ORIGIN      
        1 cagaaaaaag gccttatcag tctttaacag aaatacatca gcatctcaca ggattaccaa
       61 ctattgccga tgcaaccctt tcattacttt ctgttggatt acctggtacg ggaaaactat
      121 tgcgcaggga gcaggactat gggcgtccac cagttacagc aattcaggat tccacatttg
      181 tactccctta taatttcaaa ggtattggtt ttaacgataa cattatatcc tctgcacctg
      241 tagtctcctc attacatttt atcgctgaac atgcgaaata tactttgttg tcatggcctg
      301 agttttatcg tcatcatgca cagcgatggt tcgaaatggc taaaggatat ggaagccaga
      361 atattgattt tcaccctcag tctctattgg taacccaaga aggacgctgt atgggattag
      421 ccttacttta tttacagact gaagatactg ctcattatag cattctccag gaaaacctaa
      481 tgactgtgag tgcacttcat cagaccagta atcgcgataa gttgccactg tccaaagatg
      541 ataattcctt aatgacaaga acttatagtc tgattgaaat gctacagtat cagggaaaca
      601 aatatattac caacgaatcg ctactacata agaccgcatg gaaccaagaa agaataactt
      661 tattattcaa tgaaaaagga gttaagcgag ccctga
//
LOCUS       DAZUGK010000544          695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZUGK010000544 DAZUGK010000000
VERSION     DAZUGK010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.548"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>695)
                     /locus_tag="ACHY5W_005131"
     CDS             complement(<1..>695)
                     /locus_tag="ACHY5W_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFS4449812.1"
                     /translation="PAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYL
                     PRSATEQRKSELLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGE
                     RWIVDRYNIRHSLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLM
                     AMAVDTGGEAGVTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSA
                     RRARASGDVALWLV"
BASE COUNT          149 a          230 c          168 g          148 t
ORIGIN      
        1 accagccaca gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta
       61 tccgggaagg ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac
      121 accctgttgc ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc
      181 accccggctt cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga
      241 tcagaagcca gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc
      301 ggatatgccg ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc
      361 cggatgttgt agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc
      421 acaacaaaac ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca
      481 ccatccggca cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta
      541 cgctgttccg tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct
      601 ttcagggtct cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa
      661 atcagttgtt cccaggtctg aaacgccgca gctgg
//
LOCUS       DAZUGK010000545          691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZUGK010000545 DAZUGK010000000
VERSION     DAZUGK010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.549"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>691
                     /locus_tag="ACHY5W_005132"
     CDS             <1..>691
                     /locus_tag="ACHY5W_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4449813.1"
                     /translation="FADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSL
                     KPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLT
                     PYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQ
                     QTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQY
                     KHLFAHGEYIKG"
BASE COUNT          216 a          132 c          139 g          204 t
ORIGIN      
        1 ctttgctgat ctgccgacgt cacagttttg gtcgtacgcg acatacttca gggtattgtc
       61 ttttgaatat ctgagtgaaa gtatttccac actgctgtat ctggatgccg atgttgtttg
      121 taaaggaagc ctgaaacctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat
      181 tcctgacaat gatagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgaaatgaa
      241 tggacgttat ttcaatgcag gcgttatcta tgtcaatctt aaaaaatggc atgaagcaaa
      301 tttgacaccg tatttactca aacttttacg aggggaaact aaatatggct ctcttaaata
      361 tttagatcag gatgcgttga atatcgcatt taatatgaat aatatctacc ttgcgaagga
      421 ttttgatact atttataccc tgaaaaacga actttatgat cgtagtcatc gaaagtatca
      481 gcaaaccatt accgataaaa cagtgttgat tcactataca gggataacta aaccatggca
      541 tagctgggct ggatatccgt ctgcatcata ctttaatatc gcgcgtgaac aatctccctg
      601 gaagaaatat cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagca
      661 tctgtttgcc catggtgagt atataaaagg c
//
LOCUS       DAZUGK010000546          689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZUGK010000546 DAZUGK010000000
VERSION     DAZUGK010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.550"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            94..>689
                     /locus_tag="ACHY5W_005133"
     CDS             94..>689
                     /locus_tag="ACHY5W_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HFS4449814.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVV"
BASE COUNT          176 a          150 c          177 g          186 t
ORIGIN      
        1 gttattaatc gttgagctaa agtcattaga gatgctttgc ccttaatgta accatatcgc
       61 aataagttat gtttttaaat tgagggcatt attatgaaaa aaataccctg cgtgatgatg
      121 cgaggtggaa cctcgagggg cgcgttcctg ttagcggaac atttacccga agatcaaacg
      181 cagcgcgata aaatattgat ggcaattatg ggttccggta acgatctgga aattgacggt
      241 attggcggcg gtaatccact gaccagtaaa gtcgccatta taagccgttc cagcgatccg
      301 cgtgctgatg ttgattatct gtttgctcaa gttatcgtcc atgagcaacg tgtcgatacc
      361 acgcctaact gcggcaatat gctgtctggc gttggggcat tcgccattga aaatggtttg
      421 attgcagcga cttcgccagt tactcgcgta cgtatccgca acgtcaatac gggtacgttt
      481 atcgaagctg atgtgcaaac gccaaatggt gtagtcgagt acgagggtag cgccagaatt
      541 gacggcgtac cgggtactgc cgcaccggtt gcactcactt tcctgaatgc cgctggaacc
      601 aaaaccggaa aagttttccc gactgataat cagattgatt attttgacga tgtcccggtg
      661 acctgtatcg atatggcgat gccagtcgt
//
LOCUS       DAZUGK010000547          688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZUGK010000547 DAZUGK010000000
VERSION     DAZUGK010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.551"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..560)
                     /locus_tag="ACHY5W_005134"
     CDS             complement(<1..560)
                     /locus_tag="ACHY5W_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4449815.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDIAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERGSDEDCSSYTNGSEAREVNTDAHISGNDNSQEFSTEELLPIKHTD
                     KNIEWYKTKQWSVFISVLTVLAVCWLT"
BASE COUNT          198 a          162 c          115 g          213 t
ORIGIN      
        1 gtcagccagc acactgctaa cacggtaaga acgctaataa aaacactcca ctgcttcgtt
       61 ttataccatt caatattttt gtcagtatgt tttattggga gtagctcttc agtagagaat
      121 tcctggctgt tatcattccc agaaatgtgt gcatcagtgt ttacttccct ggcctcgcta
      181 ccattggtat agctactaca gtcttcatca ctgccacgct cattctcaca gacatgtttc
      241 tcgacctttc caagatatct gacgcctttt cgcggaatgg ttttaattag agaactcgac
      301 aaaccagcga tgtctaatgc ctttctaagc atgtaaatct gcccataata actgctgtcg
      361 cttaccgaac ctttctgctc ccgccaaacc tcattgataa tatgttgctt atcggattct
      421 ccctgtagta aaaggagtaa aaatcgatag ccgttttcag acagattaat tacattgcct
      481 gtactggtgg tgatacgtcg agacgctggg taaaacttaa ctccctgccc taaagaaaaa
      541 taaaaacggt catcgctcat aagtaccgaa ccctgttttc ttcttcacat gactactcat
      601 tacaactttg tatgaagtaa gatgcaaaat catattgttt acattaagtt aagcaattat
      661 accgtttaaa caaagacaat acgtctct
//
LOCUS       DAZUGK010000548          688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZUGK010000548 DAZUGK010000000
VERSION     DAZUGK010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.552"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(67..438)
                     /locus_tag="ACHY5W_005135"
     CDS             complement(67..438)
                     /locus_tag="ACHY5W_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS4449816.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(428..>688)
                     /locus_tag="ACHY5W_005136"
     CDS             complement(428..>688)
                     /locus_tag="ACHY5W_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4449817.1"
                     /translation="ALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDAL
                     THAEVLIDDEQFDEINIVRGQPVPGGRLGVKIYEIRGGNDGA"
BASE COUNT          198 a          194 c          113 g          183 t
ORIGIN      
        1 tgaccatcaa aaaataaaca tgttaattat aaacacagaa aacataaccc tcatcactat
       61 atatccctac cgcatatcca tatctaaccg gacattcaga gccataagca ttccttcaat
      121 aataccttcc gctttataaa gccttttacc aataagccca tcagaacatc tatgcttacg
      181 tgcaagagcc ataaatgtca ttccacctac gtaataatcc accaacaaat cgtgcaaata
      241 ctgattattc ttgtttaatc gtgccataca accacaaatt atcatggcgt catcatcaga
      301 acactgagga cgtgatttca ctttagtcgg gattaatcct ttaaaaccag cagctatcga
      361 gggccatgtt acatcttcat gattatttgc tgcccatgcc ccccatcgct caagaaccat
      421 ctggatatca cgcgccatcg ttaccacctc tgatttcgta aatcttcacg cccagccgtc
      481 cacctggcac aggctgaccg cgcacaatat tgatttcatc aaactgctcg tcatcaatga
      541 gcacttccgc atgcgtcagc gcatccagcg gtgctttcag aatgttgtcc aggtcacggc
      601 ggcgcttatc cggtggttct gcaataattt ttattgccag ccgtccggac aggcttaatt
      661 tcagccgctg ctggcgaaca ataagcgc
//
LOCUS       DAZUGK010000549          681 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZUGK010000549 DAZUGK010000000
VERSION     DAZUGK010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.553"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>681)
                     /locus_tag="ACHY5W_005137"
     CDS             complement(<1..>681)
                     /locus_tag="ACHY5W_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449818.1"
                     /translation="GFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSY
                     DRPFSAASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASL
                     EEVMTKAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGN
                     GNVAAVASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHV
                     LKSAVGAG"
BASE COUNT          129 a          216 c          181 g          155 t
ORIGIN      
        1 tccccgcgcc cactgccgat ttcaggacat gatgcagacg actgtcatcc ggatactgac
       61 gacgggccac ttccagggct tcagatcgga cgcctttagc cgcagccagc gatttggcaa
      121 agcgggcgaa gccaatcccc ttatccagtt tctgctccac acggatcacc ggcgcagaag
      181 ccaccgcggc cacattcccg ttaccggcct gtttcaccgg ctgcgccgtg gcggccttac
      241 cggcttccag ttcacgcagg cgcttcaggt gcgcatccac ctgacggatt tccgctgcgg
      301 tgttgtcgta atgctcttcc tcctccacat ccagcgtgcg cccttcctct gcggctttgg
      361 tcatgacctc ctcaagggag gctgccagcg ctgcacgctt gttttcaaaa cttttaatct
      421 gttcgccaat attcattatg gtcttttcct tatgaaaaac ggttgttgac tgtgccgcag
      481 cgccggcaga agatgcgatt ttcaccaccg gtttccggtt gccggacgcg gcagaaaacg
      541 ggcggtcgta agatttaatg gtccggatgg tgcattccgc attcgcgggc acggtgacgg
      601 cagacacctc catcagttcc cagcgcagaa aatgcagtcc gcctccgtcc agaaaggtgt
      661 attcatgggg acggaagccc a
//
LOCUS       DAZUGK010000550          676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZUGK010000550 DAZUGK010000000
VERSION     DAZUGK010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.554"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..335
                     /locus_tag="ACHY5W_005138"
     CDS             <1..335
                     /locus_tag="ACHY5W_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449819.1"
                     /translation="GRIYSNEGFSNLNWGIIGKQLELTSKDMKGFAALDPRSNHDEHD
                     DAATDMGYEIAGSATSDKNYPVKFDRRTLFFLTLKSLRENDLFTCLGAETYKAKQEAL
                     KTDQQTKR"
     gene            414..>676
                     /locus_tag="ACHY5W_005139"
     CDS             414..>676
                     /locus_tag="ACHY5W_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS4449820.1"
                     /translation="MRAVIVKSSPTSTGGKVLLGIDIVTNDGNPVALIGMQASCPACK
                     KGIGDIVAKGPHQQFINGIAIALEEDVVACGCPEGSNIVLPLT"
BASE COUNT          223 a          116 c          145 g          192 t
ORIGIN      
        1 atgggcgtat atacagtaat gagggctttt ctaatctcaa ttgggggatt attggaaaac
       61 aactcgaact tacaagtaaa gatatgaaag gttttgctgc tcttgatccg aggtcaaatc
      121 atgatgaaca tgacgatgca gccacagata tggggtatga aatagcaggt tcggctactt
      181 cagataaaaa ttacccagta aaatttgacc ggcggacatt atttttcctc acactaaaat
      241 cactcaggga aaatgatctc tttacttgtt taggtgctga gacttataaa gcaaaacagg
      301 aagctttaaa gacagatcaa caaaccaaaa gatgaagata agcgctggaa atttatgaat
      361 agtgtttatc tcataataaa aaatcaatga ttttatcaac gggagtgccg cctatgcgag
      421 ctgttattgt taaatcatca ccaacatcta caggtggaaa agttttactg ggaatagata
      481 tcgttacaaa cgatggcaac cccgttgctc ttataggtat gcaggcatcc tgtcctgcct
      541 gtaaaaaagg tatcggtgat attgttgcca aagggccaca ccaacaattt attaatggta
      601 tagcgattgc tcttgaagag gatgttgttg cctgtggctg tcctgaagga agtaatatag
      661 ttttaccatt gactaa
//
LOCUS       DAZUGK010000551          672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZUGK010000551 DAZUGK010000000
VERSION     DAZUGK010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.555"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..235
                     /locus_tag="ACHY5W_005140"
                     /pseudo
     CDS             1..235
                     /locus_tag="ACHY5W_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(316..474)
                     /locus_tag="ACHY5W_005141"
     CDS             complement(316..474)
                     /locus_tag="ACHY5W_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFS4449821.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(560..>672)
                     /locus_tag="ACHY5W_005142"
     CDS             complement(560..>672)
                     /locus_tag="ACHY5W_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449822.1"
                     /translation="NCLVLSTSFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          238 a          155 c          117 g          162 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggcaggt cataaaaaaa aggctgcgca
      121 atgcgcagcc agaactcaca aggaaaatga taaaaggaat aacactagtg atgtacgcat
      181 ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacgtgcc cttaaaactc
      241 gatcatttag cccctccaag gaggattcac catgcggttg attttttaat aaacagtaaa
      301 caaaaaagtc aagaattatt cattctgttc tttcatcatc ggccacagca ataccacaat
      361 gccgcagacc agagcgccat cagtcagtac caacattatc ctgctggtga aatccatcat
      421 caccatcact aaaagcagga tcacaacagc aagcagacac agtttataaa acaatgttca
      481 gaaaacgcat tcagcatgcc taaggttcta ttcctacgaa tagccaactt gcaacttgaa
      541 atattattta tgcagccaat taaattctgg tccttacaat atcaacctga agattcttat
      601 cttgtgctga ttgataaatg acaaaccttt tactacctgc attgaaagaa gtagacaaaa
      661 ccagacaatt at
//
LOCUS       DAZUGK010000552          671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZUGK010000552 DAZUGK010000000
VERSION     DAZUGK010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.556"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..264
                     /gene="escR"
                     /locus_tag="ACHY5W_005143"
     CDS             <1..264
                     /gene="escR"
                     /locus_tag="ACHY5W_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFS4449823.1"
                     /translation="GNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLII
                     SNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            264..533
                     /gene="escS"
                     /locus_tag="ACHY5W_005144"
     CDS             264..533
                     /gene="escS"
                     /locus_tag="ACHY5W_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFS4449824.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            533..>671
                     /locus_tag="ACHY5W_005145"
     CDS             533..>671
                     /locus_tag="ACHY5W_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFS4449825.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AF"
BASE COUNT          180 a          117 c          128 g          246 t
ORIGIN      
        1 ggtaatgaaa ctatcttaaa aaaagactct ctatttatac tgttaccggc tttcacgatg
       61 gggcagcttg aagctgcatt caagataggt tttttgctct atttaccctt tattgcgata
      121 gatttgatca tttccaatat cttattggcc ttgggtatga tgatggtatc gccagtaaca
      181 atttcgattc cttttaagat cttattattc attcttgttg gaggctggca aaaattattc
      241 gaatttctgt tggtggtgaa ttgatggata ctggatattt tgttcaatta tgtgtgcaaa
      301 cgttctggat aatatttatc ctctcattgc ctacagtcat agcggcctct gttatcggta
      361 ttattattag tctggtccag gctataacgc agttacagga tcaaacattg ccttttttgc
      421 taaaaataat agcagtgttt gctacgcttg ccctgactta tcactggatg ggaacaacaa
      481 tcatcaactt ctcgtcgata atatttgaaa tgattccgaa ggtgaacggc taatgaatga
      541 gataatgacg gtcatagtat catcatttta ttgtatactc aggccgctgg ggatgtttat
      601 tatcctacca atattttcaa caggtgtttt actcagtaac tttattcgca actcaattat
      661 gattgcgttt a
//
LOCUS       DAZUGK010000553          671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZUGK010000553 DAZUGK010000000
VERSION     DAZUGK010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.557"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..>671
                     /locus_tag="ACHY5W_005146"
     CDS             161..>671
                     /locus_tag="ACHY5W_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449826.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNA"
BASE COUNT          205 a          114 c          152 g          200 t
ORIGIN      
        1 ttcaggtaat atttaaatat tcatgaaatc tataaattaa agatttgtca cttattggat
       61 ttagtatgct tgatttaaat ggcggacaat tattgttatt attaagtcta atgtcattta
      121 aaggcattat catatttacg aagagatcag gatgataacg atgaaaaaaa gtgtattgac
      181 ggcgtttata actgtggtat gtgcaacgtc cagtgttatg gcagctgatg ataatgctat
      241 cacggatggt tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtcgc
      301 tgcgacgaaa gattccgtgg tgactttgcc agatgttagt gccacgaagt tgcaaaccaa
      361 tggtcaggtt tctggcgtgc aaactgatgt gccaattgaa ttaaaagatt gtgatactac
      421 cgtaacaaaa aatgcaacgt tcacctttaa tggcactgcg gatactactc agattacagc
      481 gtttgctaac caggcctcat ctgatgctgc tacaaacgtg gccctgcaaa tgtatatgaa
      541 tgatggtaca acggccatca cgccagacac agaaaccggg aacattttgt tgcaggatgg
      601 agatcagacg ttgactttta aagttgatta tatcgctacg gggaaagcga cttcaggtaa
      661 tgtgaatgcg g
//
LOCUS       DAZUGK010000554          669 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZUGK010000554 DAZUGK010000000
VERSION     DAZUGK010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.558"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..>669
                     /gene="espY1"
                     /locus_tag="ACHY5W_005147"
     CDS             87..>669
                     /gene="espY1"
                     /locus_tag="ACHY5W_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFS4449827.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKIDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFINSLELFNELKTCA
                     KNPDEMVAKGNMRWVAEIYGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRNCYFGKKTMKGKDEILYEQPGWNANLDG"
BASE COUNT          213 a          116 c          161 g          179 t
ORIGIN      
        1 aacacatatc tgaatgagcg atttttgaaa gtatatttat tcagagcgcg catcatgagt
       61 ttttaactca atgcgaggct attaccatga aagtatcagt tccaggcatg ccggctacgc
      121 ttttaaatat gagcaataac gatatttata agatggtgag cggggacaag attgacgtga
      181 agatgaatat ctttcaacgc ttgtgggaga cgttacgtca tctgttctgg tcagataaac
      241 agactgaggc ttataaactt ctgttcaatt tcgtgaataa aaaggctggc aacatcaacg
      301 cctcaaaata ctttactggg gctgtcaacg agaatgagaa agaaaagttt atcaatagcc
      361 tggaattatt caatgaactc aaaacatgcg caaaaaatcc ggatgagatg gtcgcaaagg
      421 gcaatatgcg ctgggtcgcc gagatctacg gtgatatcga gttaagtgtc acttttttca
      481 ttgaaaataa ggagatatgt actcagacgt tgcagctgca taaaggccca ggtaacctgg
      541 gcgttgatct tggagaggct taccttcccg gcgttgacat gaggaattgt tactttggta
      601 aaaaaacaat gaaaggtaaa gatgaaatcc tttatgagca acctgggtgg aatgctaacc
      661 tggacggtg
//
LOCUS       DAZUGK010000555          664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZUGK010000555 DAZUGK010000000
VERSION     DAZUGK010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.559"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..520
                     /gene="nleB"
                     /locus_tag="ACHY5W_005148"
     CDS             <1..520
                     /gene="nleB"
                     /locus_tag="ACHY5W_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFS4449828.1"
                     /translation="LREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFT
                     ETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVV
                     NRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENI
                     IPNTSMYTSSSW"
     gene            569..>664
                     /locus_tag="ACHY5W_005149"
     CDS             569..>664
                     /locus_tag="ACHY5W_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFS4449829.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKH"
BASE COUNT          214 a           99 c          139 g          212 t
ORIGIN      
        1 attaagagaa gagttaaaaa atatcccaga aggtaaggac tctttgattg agtcatatgc
       61 agaaaaaaga gaacatactt ggtttgattt tttcaggaat ttggccatgt tgaaggctgg
      121 aactttgttt acagagacgg gaaaaactgg atgccataac atatcgccct gtagcggatg
      181 tatatatctt gatgccgaca tgattattac cgataaatta ggagtcctgt atgctcctga
      241 tggtatcgct gtgcatgtag attgtaatga tgagataaaa agtcttgaaa atggtgcgat
      301 agttgtcaat cgtagtaatc atccagcatt acttgcaggc ctcgatatta tgaagagtaa
      361 agttgacgct catccatatt atgatggtct aggaaagggt atcaagcggc attttaacta
      421 ttcatcgtta cacgattata atgctttttg tgattttatt gaatttaagc atgaaaatat
      481 tataccgaat accagtatgt ataccagcag ttcatggtaa gtcttcctgt tgtggttgaa
      541 aaatatcgtt ctttaggtat agattcagat gattaatcct gttactaata ctcagggcgt
      601 gtcccctata aatactaaat atgctgaaca tgtggtgaaa aatatttacc cggaaattaa
      661 acat
//
LOCUS       DAZUGK010000556          664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZUGK010000556 DAZUGK010000000
VERSION     DAZUGK010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.560"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>664
                     /locus_tag="ACHY5W_005150"
     CDS             <1..>664
                     /locus_tag="ACHY5W_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449830.1"
                     /translation="EAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSILVNKTPLTDTD
                     GNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRTITSANIDRLRV
                     TFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNL
                     PPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQ
                     QMT"
BASE COUNT          163 a          173 c          209 g          119 t
ORIGIN      
        1 gaggcaaagg acaatcttaa gtccacgcag atgatgagcg tgattgatgc gattggtgag
       61 ggaccgatag aaggcccggt gaagggactg cagagtatcc tggtgaacaa aaccccgctg
      121 acggacacgg acggtaatcc cgtgatacac ggtgtgactg cggtctggcg tgccggggag
      181 caggagcaga caccgccgga aggttttgag tcatccggct ctgaaaccgc actgggcgtg
      241 gaagtgacga aggcaaagcc ggtgacgcgc accattacat ccgcgaacat tgaccgcctg
      301 cgggtcacct tcggggtgca gtcactggtg cagaccacgt caaagggtga ccgaaacccg
      361 acatccgtcc gcctgctgat tcagttacag cgtaacggta actgggtgac ggaaaaggat
      421 gtcaccatta acggcaagac cacctcacag ttcctggcct cggtgattct ggataatctg
      481 cctccccgtc cttttaacat ccggatggtc agggagacgg cggacagcac cacggaccag
      541 ctgcagaata agacgctgtg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
      601 ccgaacacgg ccattgtggg gctgcaggtg gatgcggagc agtttggcgg tcagcagatg
      661 acgg
//
LOCUS       DAZUGK010000557          663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZUGK010000557 DAZUGK010000000
VERSION     DAZUGK010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.561"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..>663)
                     /locus_tag="ACHY5W_005151"
     CDS             complement(40..>663)
                     /locus_tag="ACHY5W_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFS4449831.1"
                     /translation="SVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHF
                     PEQIAPQERDPQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDA
                     DPTFDFIGYLETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFG
                     LGLPVMLKEYGLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDAPATT"
BASE COUNT          125 a          175 c          200 g          163 t
ORIGIN      
        1 cagggataaa aaaagaccgg cagatgccgg tcaggtgggt caggttgttg caggggcgtc
       61 acattttggc agccagtcgc cgtagctttc ctctttcagt gtcaggttgg tctgtatccc
      121 ctgtttggta tggcgcttct cgtaattcag tccgtattcc ttcagcatca ccggcagccc
      181 cagcccgaac attttcagac tgagtacatt ccggtagccg tttgcctcca tgtaggccag
      241 ataggcgtga tagaggtatt tacggtaatt acgcgggatg atactggcgt tccccatata
      301 catgccgctg gtctgcggca gggtttccag atagccgata aaatcaaacg tcgggtcggc
      361 atcccgtttg atgttcagtg cctcgtctga gttctgctgg gactgaagca gtgaccgggc
      421 gagcatcggg tcgctgaact tctgcatcag gtgacgcacg atgaccgcca gctcgcgggt
      481 gattttgtcc ttaagctgcg ggtcgcgctc ctgcggggct atctgttccg ggaagtgaat
      541 aatcacccgc cggcgtgaca cgccgccgct gcggtcggtg aagcgcatcg ggttattgtt
      601 cacggccaga atcaccgccg ggatatgcgt ggagtacgca tcccggtatt tcgggtcaac
      661 gga
//
LOCUS       DAZUGK010000558          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZUGK010000558 DAZUGK010000000
VERSION     DAZUGK010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.562"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            57..>658
                     /gene="nleG"
                     /locus_tag="ACHY5W_005152"
     CDS             57..>658
                     /gene="nleG"
                     /locus_tag="ACHY5W_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFS4449832.1"
                     /translation="MPVILNFSSERVLPESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIE"
BASE COUNT          201 a          143 c          133 g          181 t
ORIGIN      
        1 atcttgactc ctgtcaccaa tatggttcaa taacatagac taaagaggga gaaaatatgc
       61 ctgtcatatt aaacttttcg agtgaaagag tattgcctga aagtgaactg gaggctttac
      121 gtcatgtcgg aagagttagt cagagtgaac agcttgttgt gagaggccgc actatgagac
      181 ttcatcatat ttcatttatg gacagtttta gcgtggaacc ggtttccggc ggactgttgg
      241 atcgccttag tgcaagaggg catcgccttc tcgcagaaaa tctggagata cagcttaaca
      301 gaggccatac ttttttgcag gcattccgtt tatatatgga acagagccgt gcaaccccat
      361 gtacgcgcca aaacgtcagt agtgcaatac aaaacaaaat taactctcat gcatttactg
      421 ttagtcatca agacttttcc tgtcatgaac aacacctgaa ttgcccgata acattatgca
      481 ttcctgaaac tggtgttttt gtcagaaatg caaaaaactc agaaatatgt tcattatatg
      541 accacaatgc actcactgaa cttatccgac gcaacgctcc acaccctctc agccgtgaac
      601 cctttgttcc ggaaatgatt gtcagcaaag atgaatgcca ttttaatcta atagaaca
//
LOCUS       DAZUGK010000559          652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZUGK010000559 DAZUGK010000000
VERSION     DAZUGK010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.563"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..>652)
                     /gene="fimA"
                     /locus_tag="ACHY5W_005153"
     CDS             complement(126..>652)
                     /gene="fimA"
                     /locus_tag="ACHY5W_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS4449833.1"
                     /translation="SSALAAALFAATGSYAAVVDGGTIHFEGELVNTACSVNTDSADQ
                     VVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAINDNLLAIA
                     SSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGTGTSASA
                     GQANADATFIMRYE"
BASE COUNT          194 a          137 c          147 g          174 t
ORIGIN      
        1 taataaagcg gcatgataca aagtgaacat gatttcgatt tacattgcca ccataccgct
       61 tcagacaacc ggtacattac tttgttataa gacgtggtat ataattcaaa acaacgtggt
      121 tttgattatt cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca
      181 cttgtaccgg tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag
      241 ttctggttgg ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt
      301 atttcaatac cgacacccgt tgctgttgtt gtattggtac tggatgcaat ggccaataaa
      361 ttatcattga ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca
      421 ggatcgcagt cgttcaactg tatagtgaat ggaattaatg cagaggtatt accaacagca
      481 ttgaaaatat cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc
      541 actgaacagg cagtattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg
      601 gcagcataac tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aa
//
LOCUS       DAZUGK010000560          651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZUGK010000560 DAZUGK010000000
VERSION     DAZUGK010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.564"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..521)
                     /locus_tag="ACHY5W_005154"
     CDS             complement(<1..521)
                     /locus_tag="ACHY5W_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFS4449834.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGA"
BASE COUNT          189 a          146 c          129 g          187 t
ORIGIN      
        1 gcaccacctt tgccgtcatt agcgatgtta ttgcgaaaca cggcgttagt gacacgtaaa
       61 tcgatggcac cggtatcgtt agtaccagaa gaatagattg cgccaccata gccgcctgcg
      121 acgttaccag aaaaaataac atccgtaaga ttcagcgttg agttttcttt agcaaatatt
      181 gccccgccgt tattatattc tcctgaaacg gtgttattgg caaacagagt cattccagtt
      241 tcattttctg gtgataatgt aaattccgct ccttgttgca aaaatacggc cccaccgctg
      301 gcattattgg tcatatccga aaaaaccagc cattgcccat cggcgatact ccagacctgc
      361 gtaatgcctg aaaggctttg cctgctggcg tgacaactcg ctttgatatc atatccctgg
      421 cataaatcaa tggccgccgc gacaccgtta gcagagaaaa gagatgcaat cattgacggg
      481 agtagagata aatactcctt gcgtagaaag ataatccgca tattaatctg accattattt
      541 tatataagac ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt
      601 gtccaacccg atgcaatagc tattttgtta ttatttaatg aatagtatca g
//
LOCUS       DAZUGK010000561          650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZUGK010000561 DAZUGK010000000
VERSION     DAZUGK010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.565"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..94
                     /locus_tag="ACHY5W_005155"
     CDS             <1..94
                     /locus_tag="ACHY5W_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449835.1"
                     /translation="CLVHIGKNLPAEKKAEFLATLIAMKSKGEI"
     gene            196..>650
                     /locus_tag="ACHY5W_005156"
     CDS             196..>650
                     /locus_tag="ACHY5W_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS4449836.1"
                     /translation="MSTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYKKGGEDGITMVFIAPS
                     SSAQDKPASTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPV"
BASE COUNT          208 a          141 c          152 g          149 t
ORIGIN      
        1 gtgcctggtg catatcggaa agaatctgcc cgcagaaaaa aaagccgaat ttctggcaac
       61 tctgattgca atgaaatcaa agggtgaaat ctgaaaaaaa agaaagcctg cacactgtgc
      121 aggcctgagt gaagaacctg ggacatttat tcatcactcg cagtaatttt aatctaagtt
      181 gaggttaaaa aaacaatgag caccgataaa caagtttacc cactgtatta cgaagcaaaa
      241 aatgacaaag taagaaaacg tctcggtatt aaaggcggtt tctactgggc tgaagcgaaa
      301 aaattatcca ttgccatatc ccgtggtgct gttgcgattg acgatgctgg ctacgatgaa
      361 gatgacttta aaaaacctgt tcgcgtcaat ttgcccgttg ttgatgacct tccaccagag
      421 ggcgtatttg atacggaatt ctgcaaccgt tacaaaaaag gcggggaaga tggcatcaca
      481 atggtattta tcgcgccctc atcctctgcg caggacaaac cagccagcac tgacaatacc
      541 aatgttaatg gcgaagacat gacggagatt gaggagaata tgctactccc gatttccggt
      601 caggagctgc ccattcgctg gcttgctcaa cacggcagcg aaaaaccggt
//
LOCUS       DAZUGK010000562          650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZUGK010000562 DAZUGK010000000
VERSION     DAZUGK010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.566"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHY5W_005157"
     CDS             <1..95
                     /locus_tag="ACHY5W_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449837.1"
                     /translation="CLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            196..>650
                     /locus_tag="ACHY5W_005158"
     CDS             196..>650
                     /locus_tag="ACHY5W_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS4449838.1"
                     /translation="MSTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     SSAQDKPASTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPV"
BASE COUNT          204 a          140 c          153 g          153 t
ORIGIN      
        1 tgtgcctggt gcatatcgga aagaatctgc ccgcagaaaa aaaagccgaa tttctggcaa
       61 ctcttattgc aatgaaatta aaaggtgaaa tctgaaagaa atagcctgcg tatggcacag
      121 gctatgaaca gtgtgtatcc ggcaagatta ttcactgaac aaacgaattt taatctgagt
      181 tgaggttaaa aaacaatgag caccgataaa caagtttacc cactgtatta cgaagcaaaa
      241 aatgacaaag tcagaaaacg tctcggtatt aaaggcggtt tttactgggc tgaagcgaaa
      301 aaattatcca ttgccatctc ccgtggtgct gttgcgattg atgatgctgg ctacgatgaa
      361 gatgacttca aaaaacctgt tcgcgtcaat ttgcccgttg ttgatgacct cccgccagaa
      421 ggcgtatttg atacggaatt ctgcaaccgt tacgaaaaag gcggggaaga tggcatcaca
      481 atggtattta tcgcgccctc atcctctgcg caggacaaac cagccagcac tgacaatacc
      541 aatgttaatg gcgaagacat gacggagatt gaggagaata tgctactccc gatttccggt
      601 caggagctgc ccattcgctg gcttgctcaa cacggcagcg aaaaaccggt
//
LOCUS       DAZUGK010000563          646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZUGK010000563 DAZUGK010000000
VERSION     DAZUGK010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.567"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..551
                     /gene="ydeO"
                     /locus_tag="ACHY5W_005159"
     CDS             <1..551
                     /gene="ydeO"
                     /locus_tag="ACHY5W_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFS4449839.1"
                     /translation="VPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQSHMGMNTGNTMNALAI"
BASE COUNT          193 a          129 c          133 g          191 t
ORIGIN      
        1 cggtgcctgt tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg
       61 aaatgattgc ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc
      121 ttttttcttg cctgtctata tttgccgcat gcaaaggttt cattacacta ttaactaacg
      181 gtgtgctatc cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat
      241 ggaagctgaa agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac
      301 taaagcaaga gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa
      361 aaaatttgat acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca
      421 gtacatctta ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt
      481 ctaaggagta ccgttgtcaa agtcacatgg gtatgaatac gggcaacacg atgaatgctt
      541 tagctatttg attatttgct aacgagtagt caaccacgca ccctacgtaa aaattaaaaa
      601 tggggcagtc attccctgcc ccgcgttgtt tttaggcgat atattt
//
LOCUS       DAZUGK010000564          644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZUGK010000564 DAZUGK010000000
VERSION     DAZUGK010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.568"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..>644
                     /gene="escD"
                     /locus_tag="ACHY5W_005160"
     CDS             84..>644
                     /gene="escD"
                     /locus_tag="ACHY5W_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFS4449840.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTT"
BASE COUNT          179 a          138 c          131 g          196 t
ORIGIN      
        1 tcctttctac tctattctta gacgatgtaa gttcaccata ttttttctca ttgttcaacc
       61 accaggatga ataaaattta aaaatgttat cctcatataa aataaaactt ctcaatggtg
      121 cgatgcgtaa tcgggaatta caacttccga tgggaaacct aacgctaggt acggaagata
      181 acgatattgt ttactttccc cttgaacaag gcctcaatca gtttttactt gatatacgtg
      241 aggaaggtgt tttcctttta tctccggttg aattttggat tgatgggcaa cccacgccct
      301 atgaagcaga taaaccatta cctgtcggaa aggttattga tatagcggga tgttgcttca
      361 tcattggtga tattgaccat tccttgccat tgtctgatgt gccagaaagg ttctctgcca
      421 aaagtcgacg caaaaaacgc cttatcctgg cgagcgtgat tggtgctact tttgcgttgt
      481 ctggtgccat cggcagttac gttttgcttt ccccaaaagc tgaaccccca acgtttacca
      541 gagctgatgt atatcagcag ctaaaagaaa acaaacttca tgcaatcact ctggtatggc
      601 atgggaaaaa tatcgcgctt tatgggcgtt gcgaatcaac cacg
//
LOCUS       DAZUGK010000565          644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZUGK010000565 DAZUGK010000000
VERSION     DAZUGK010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.569"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>644
                     /locus_tag="ACHY5W_005161"
     CDS             <1..>644
                     /locus_tag="ACHY5W_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4449841.1"
                     /translation="SIVNEVVKTYSLPIEKDDNGYYDFSGLYLAHSNISGKDPNKDFG
                     IDLGNCNCSNVNFEHTYFDSVKFTNTNCTNANFNSCRFIKCDLTNMNCTGAILDNAVI
                     YGKEKEPEMQYPEADQIIQRITYQKSHGNETKGMILTNCSCVKTTFNWADLSESNCQN
                     VDFSEANLSNTILPDIAMMKDTKLYRTDLFNPILKAEAESTEEKDISPLAKIIL"
BASE COUNT          241 a          114 c          113 g          176 t
ORIGIN      
        1 tctctattgt taacgaggta gtaaagactt attcactccc aatagaaaag gatgacaatg
       61 gatattacga tttcagcggt ctatatctag cacacagcaa tatttccggt aaagatccca
      121 ataaagattt cggtatagat ctgggaaact gtaactgctc aaatgtcaac ttcgaacata
      181 cttattttga tagcgtcaag tttacaaata cgaattgtac aaatgcgaat tttaacagtt
      241 gtagatttat caaatgcgat ctaacaaata tgaattgcac tggcgcaatt cttgataatg
      301 cggtgatata tggtaaagaa aaagaacctg agatgcaata tccagaagca gatcaaataa
      361 tacaaaggat aacttatcag aaatcacatg ggaatgaaac gaaagggatg attctaacta
      421 actgctcctg tgtgaaaacc actttcaatt gggcagattt atcagaaagt aattgccaaa
      481 atgtggactt tagcgaggct aatctttcaa acactattct tcctgacatc gccatgatga
      541 aagacacgaa actttaccgt accgaccttt ttaatccgat attaaaagca gaagcagaat
      601 caacagaaga aaaagacatt agcccattag caaaaattat tctt
//
LOCUS       DAZUGK010000566          641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZUGK010000566 DAZUGK010000000
VERSION     DAZUGK010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.570"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..>641)
                     /gene="map"
                     /locus_tag="ACHY5W_005162"
     CDS             complement(34..>641)
                     /gene="map"
                     /locus_tag="ACHY5W_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFS4449842.1"
                     /translation="SPMTMAGRSLVQATAQTLRPAVTRAAMQAGTGATGMRFMPVQSN
                     FVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVISK
                     ETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFNTQ
                     TKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          154 a          148 c          130 g          209 t
ORIGIN      
        1 aggaagctta atttatacct gacaaggtta tccctacaat cgggtatcct gtacatgctc
       61 taaaatcgca ttctgagtaa tcgcttttat ctcactgact ttgaagccgc taaagttgcg
      121 ttcaaatatt tcttgtgaga cttgttctat catcttcgtc tgggtgttaa aacgtgttat
      181 tggatccact cttttgccgc actgctcatc aactttctga ttaaagtatt tattggactg
      241 caagaagtga ccaattgatg attgtgcccc gttcatatca agaggttgct gtaacgcatt
      301 ttcaacagct ctaaaaatgt gacctttagt ctccttacta atgaccgaat tattactcct
      361 gcaatagtca tccagagttc tatttactgt tctggatata taagtcgtct gctcctgctg
      421 gaaccattgt tgggtatcgc cattacgagt ttgtttggct acagcctgca ggagttggtt
      481 agtcagtttg ccatgattaa tcacaaagtt cgactgcacg ggcatgaacc tcatacccgt
      541 ggctcccgta cccgcttgca ttgcagctct ggtaaccgca ggtctaagcg tctgtgcagt
      601 cgcctgaact aacgatctgc ctgccattgt cattggacta a
//
LOCUS       DAZUGK010000567          639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZUGK010000567 DAZUGK010000000
VERSION     DAZUGK010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.571"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..639
                     /locus_tag="ACHY5W_005163"
                     /pseudo
     CDS             54..639
                     /locus_tag="ACHY5W_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          177 a          105 c          142 g          215 t
ORIGIN      
        1 acaaaactgt aacaaaaaac aaaataaaaa accatggaag aaggtgatgt tcaatgttta
       61 acaaagtttt ggtagggtta aggagtcatc cagaattaat aattcttgga ttaatggtga
      121 tgattatcgc catgctcata attccattgc ccacctactt aatagacttt ttgattgggc
      181 ttaacttaac attggcaata cttgtatttt taggatcatt ctatgttgat aggatcttaa
      241 gcttttcttc atttccatca atcctcctta tcacaacgtt atttcgtttg gcgctggcga
      301 tcagtaccag tcgacttata cttcttgagg cggatgctgg agaaattata acgagttttg
      361 gtgagttcgt tattggagat agcctggtag ttggttttgt tattttctcc attgttacta
      421 ttgttcagtt tatcgtgatt actaaaggtt cggaacgtgt ggctgaagtt gctgccgctt
      481 ttcgcttgac ggtatgccag gtaaacaaat gagcattgat gccgatctac gcgctgggat
      541 cattgatgca gatttagcaa aagaacgtcg tagcgtatta gaacgagaga gtcagctgta
      601 tggttccttt gatggtgcga tgaaatttat caaaggcga
//
LOCUS       DAZUGK010000568          638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZUGK010000568 DAZUGK010000000
VERSION     DAZUGK010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.572"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..173
                     /locus_tag="ACHY5W_005164"
     CDS             <1..173
                     /locus_tag="ACHY5W_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449843.1"
                     /translation="YRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMVAN
                     FGGFLSINKLSQ"
     gene            184..>638
                     /locus_tag="ACHY5W_005165"
     CDS             184..>638
                     /locus_tag="ACHY5W_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4449844.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMSGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNL"
BASE COUNT          124 a          154 c          217 g          143 t
ORIGIN      
        1 cctaccgctc tgatgagtgt ggttacacgg gcggggctgt ggcggatgag ttcgataaac
       61 ccaccaccga tatccgtaag gacagatgca gcaagtgcat gcgcgggtgt gagatgcgcg
      121 gcatggtggc taattttggc ggtttccttt ccattaacaa actttcgcag taaatcccgg
      181 tttatgacac agactgaatc agcgattctg gcgcatgccc ggcggtgtgc gcctgcggag
      241 tcgtgcggct tcgtgataag cacgccggag ggggagcggt atctgccgtg tgtgaatatt
      301 tccgcagagc cggaggcgta ttttcgtatc gcaccggaag actggctgcg ggcagagatg
      361 tcgggggaga ttgtggcgct ggttcacagt catcccggtg gtctgccctg gctgagcgag
      421 gctgaccggc ggctgcagat aaaaagcgca ctgccctggt ggctggtcag ccggggtgac
      481 attcacaaat tccgctgtgt gccacatctg acaggacggc gctttgagca cggggtgacg
      541 gactgttaca cgctgttccg ggatgcatac catctggcgg gaattgatat gccggatttt
      601 gagcgtgagg atgactggtg gcgcaatggt cagaacct
//
LOCUS       DAZUGK010000569          635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZUGK010000569 DAZUGK010000000
VERSION     DAZUGK010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.573"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>635
                     /locus_tag="ACHY5W_005166"
     CDS             <1..>635
                     /locus_tag="ACHY5W_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449845.1"
                     /translation="GVFSSFHLSELENMSPDDLHEEYGNISLFNWVHAYQCLVELSKE
                     EMSKRFSSTKPIPLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDL
                     NDCPFIPCMDGLCLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAG
                     IKASPIDAHANYQCDCVILLDDCLIFTELKSNGQPIYYGKYYQQVCNIVGD"
BASE COUNT          201 a          108 c          122 g          204 t
ORIGIN      
        1 atggcgtttt tagctcattt catctaagcg agttggaaaa tatgtctcct gatgaccttc
       61 atgaagaata tgggaatata tcattattta attgggtgca cgcttatcaa tgtttagtcg
      121 agttatctaa agaagaaatg agcaaaagat tttcatcgac aaaacctata cccctacagt
      181 tggatcgctg gctgatcata aaaagccgtg agagttggtt atcattcttt caacgtaagg
      241 ggattgcagc agatgcagca aaaaaactga tagattattt taccttcaat tcaaaatcac
      301 atgatcttaa tgattgccca tttattccct gtatggacgg tttatgctta atgcctgcat
      361 taatagcaaa tagctcagtg actcgctctc taatgtcatt atttggttcc aaaaaaattt
      421 ctcaagcaag taaagggcga tttcatgagc aacaatttat aaaacaagtt cgtgatgcgg
      481 ggattaaagc ttctccaatt gatgctcatg ctaattatca gtgcgattgc gtgattctcc
      541 tggatgattg tttgattttt acagaactaa aatcaaatgg tcaaccaata tattatggta
      601 agtactatca gcaagtgtgt aacatcgttg gagat
//
LOCUS       DAZUGK010000570          635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZUGK010000570 DAZUGK010000000
VERSION     DAZUGK010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.574"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..558)
                     /gene="fimE"
                     /locus_tag="ACHY5W_005167"
     CDS             complement(<1..558)
                     /gene="fimE"
                     /locus_tag="ACHY5W_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFS4449846.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERN"
BASE COUNT          186 a          167 c          130 g          152 t
ORIGIN      
        1 atttctttcc cataatccag caaaacgagc agcattactg gcggtataac gcacagtatg
       61 gcgaatattt cgatgcccga gataatcctg aattaaacgg gtatctgcac cacgctccgc
      121 cagttcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta cggttccagc
      181 ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc gactcccgcg
      241 gcgagaaata aatatagcgt cggtccggtc agcgcctttc cagttagcac gttcctgggt
      301 ccagcgttcc acagcttcac gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt
      361 cttcagtcgg cgaatattta ttcgaccttc attaaggtca agatcctgat aatgcagatc
      421 aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac aataatctct
      481 ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt taccggtaag
      541 ataacgacgt ttactcacaa tagtagtact cctaactgag atatatttga atgaatacct
      601 acaggaaacc tcaatcggtc aaaattagcc caata
//
LOCUS       DAZUGK010000571          623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZUGK010000571 DAZUGK010000000
VERSION     DAZUGK010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.575"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..491)
                     /locus_tag="ACHY5W_005168"
     CDS             complement(<1..491)
                     /locus_tag="ACHY5W_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS4449847.1"
                     /translation="MQDIHEETLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFP"
     gene            complement(491..>623)
                     /locus_tag="ACHY5W_005169"
     CDS             complement(491..>623)
                     /locus_tag="ACHY5W_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4449848.1"
                     /translation="GWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT          131 a          230 c          140 g          122 t
ORIGIN      
        1 ggaaacagcg ccccgtccgt ctcggtcggt gtggccagca caaacgaggc cgtcatggcc
       61 gtcagcgatg acatctgctc caccacccag cggtctctca gctcctgctc cgggtccgct
      121 tccggattgc ctgccacaaa gttcaccgca tccagaaaac gggcatacac ccggcggcgg
      181 accaccgtgg cacccaccag gctctgcaaa tcctccgcca tcccggtgac aagaccgaac
      241 agattggaca ccgtcagcga cgggcgggca ctgctgccct ttccgttcat ctcaaagcca
      301 ctcccctcaa tcgggtacgc ctgatattca cgcccctgcc aggtcaccgg ctcccctttt
      361 tcattcagct cattgcagaa aaaataccgc tcaccgccct gcaccgtcag gtcgatttcc
      421 cagagtacca cccgcggtga ctgctctgac ttaaccgact cgttaagagt ttcttcgtga
      481 atatcctgca tcagttcacc acctgcttaa actccgcgct gaactcaacg cgcaacatcc
      541 cgacccgcgc agaccacccg gcacaggtca cctttatctg ccggtatgca tagggtggct
      601 tccacaaaaa tgccttccag cct
//
LOCUS       DAZUGK010000572          623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZUGK010000572 DAZUGK010000000
VERSION     DAZUGK010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.576"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..266)
                     /gene="tnpB"
                     /locus_tag="ACHY5W_005170"
     CDS             complement(<1..266)
                     /gene="tnpB"
                     /locus_tag="ACHY5W_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449849.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLT"
     gene            complement(263..>623)
                     /locus_tag="ACHY5W_005171"
     CDS             complement(263..>623)
                     /locus_tag="ACHY5W_005171"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449850.1"
                     /translation="GRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGINANLLFK
                     WRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFN
                     GNVSEKLLTLLIQELKR"
BASE COUNT          136 a          164 c          178 g          145 t
ORIGIN      
        1 gtaaggaata ctttgccatc acgggctgac ggccaggcga agcgcccacg ctccagccgt
       61 ttggtcagga ggcacagtcc gtcaccggtg gaccacagca gtttaacctg actgccgctg
      121 cggccccgga aaatgaaaac atggccggac atgggatcgt ctttcagcgc cgtctgtact
      181 ttcgcagcca ggccgttgaa gccatttctc atatcggtga taccggcaac cagccaaatt
      241 ttggtcccgg aaggtaacgg gatcatcgct tcagttcctg tatcagcaga gtcaggagct
      301 tttcgctgac attgccattg aagcggagcg tcccgtgccg gaacgttacc tcacagctga
      361 tactgagggt ttccgggtcc tctgcgagcg attctggctg ttcggcagct gcatcgagag
      421 tcacaggaag tagctggggg ctctctgaag aaggtaatag cagctttccc tcgcgccatt
      481 gttgtcgcca tttgaacaac agattggcgt taatgccatt ttcaagagca agttttgaga
      541 tggatatccc gggttcacag gaggcagcaa cgagctgctg tttaaattcg ggaggataat
      601 tagggcagcc ttttcgcctg ccg
//
LOCUS       DAZUGK010000573          621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZUGK010000573 DAZUGK010000000
VERSION     DAZUGK010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.577"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHY5W_005172"
     CDS             complement(<1..140)
                     /locus_tag="ACHY5W_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4449851.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..546)
                     /locus_tag="ACHY5W_005173"
     CDS             complement(121..546)
                     /locus_tag="ACHY5W_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS4449852.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          139 a          203 c          139 g          140 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
      601 gtcgcttctg ctttcccggt t
//
LOCUS       DAZUGK010000574          620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZUGK010000574 DAZUGK010000000
VERSION     DAZUGK010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.578"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>620
                     /locus_tag="ACHY5W_005174"
     CDS             <1..>620
                     /locus_tag="ACHY5W_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin hemolysin A"
                     /protein_id="HFS4449853.1"
                     /translation="GIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIE
                     GNYGNDRLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGA
                     YNILSGGTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKG
                     DRLHLSDISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFK"
BASE COUNT          195 a           67 c          167 g          191 t
ORIGIN      
        1 ggaattcctt atgatgtaat agataatctt cattctgttg aagagctcat tggcggaaaa
       61 catgatgatg aattcaaagg cggtaagttt aatgatatat tccatggcgc agatgggaac
      121 gattatatcg aaggtaatta tggtaatgat cgactatacg gcgatgatgg ggatgattat
      181 atatccggag gacagggaga cgaccagtta tttggtggta gtggaaacga taaattgagt
      241 ggaggggatg gtaataatta tctgacagga ggaagcggta atgatgagct tcaggcacac
      301 ggagcttata atattctgtc aggtggtact ggtgatgata aactttatgg tggtggtggt
      361 attgatcttc tggatggagg ggaaggtaat gactatctga atggtggttt tggtaatgat
      421 atttatgttt ataggcaaaa ctatggtcat catacaattg cagatgaagg aggtaaagga
      481 gatcgtttgc acttatctga tattagcttt gatgatatcg catttaagag agttggaaat
      541 gatcttatca tgaataaagc cattaatggt gtactttcat ttaatgagtc aaatgatgtc
      601 aatgggataa catttaaaaa
//
LOCUS       DAZUGK010000575          612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZUGK010000575 DAZUGK010000000
VERSION     DAZUGK010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.579"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..173)
                     /locus_tag="ACHY5W_005175"
     CDS             complement(<1..173)
                     /locus_tag="ACHY5W_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449854.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHY5W_005176"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHY5W_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          134 a          197 c          136 g          145 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg atgccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgcccttcct cagcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacgtc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcgggtctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcac cttcgaatgc ttttttgatg ttaccggctc tgtcgcttct
      601 gctttcccgg tt
//
LOCUS       DAZUGK010000576          611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZUGK010000576 DAZUGK010000000
VERSION     DAZUGK010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.580"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>611
                     /locus_tag="ACHY5W_005177"
     CDS             <1..>611
                     /locus_tag="ACHY5W_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS4449855.1"
                     /translation="MDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMT
                     EDDARPEALRRFEQMVEEAARHAGEAKMNAGEAETSARNAGISASKAEASAANADTSA
                     EDASESARQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGN
                     ASRDATTATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPG"
BASE COUNT          169 a          153 c          195 g           94 t
ORIGIN      
        1 atggacgttg agtacggtca gtacagcgtt attctgttgg tggagggatt cccgccgtca
       61 catgccggga ccatcaccgt gtatgaagat tctcaaccgg gtacgctgaa tgattttctc
      121 ggtgccatga cggaggatga tgcccgtccg gaggctctgc gtcgttttga acagatggtg
      181 gaagaagcag cgcgtcacgc cggggaggcg aagatgaatg ccggagaagc agagacgtcc
      241 gcgaggaatg ccggcatatc agccagtaag gcggaagcga gcgccgcaaa tgctgatact
      301 tcagcagagg atgcatcgga gtcagcccgg caggcggcag aaagtgcagc ctctgcaaag
      361 aagtcagagg aagcgtcctc gtcctcagcc tctgaggccg ctcaaaaagc cagtgagtca
      421 ttacaaagtg caacagatgc cgagttgtca aaaaagacgg cagaaagtgc agccggtaat
      481 gcatccagag acgcaacgac agcaacagaa aaagcccggg agtcagcaga aagcgcacag
      541 tcagcggaac aaagcaggat agcggcggaa gaagccgtaa accgaatccc caccgtggtg
      601 gggccacccg g
//
LOCUS       DAZUGK010000577          609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZUGK010000577 DAZUGK010000000
VERSION     DAZUGK010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.581"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>609
                     /locus_tag="ACHY5W_005178"
     CDS             <1..>609
                     /locus_tag="ACHY5W_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449856.1"
                     /translation="FPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKTASEARLRF
                     FDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNALLGLANVY
                     NGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATIPYDNDNES
                     NKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRS"
BASE COUNT          239 a          114 c          114 g          142 t
ORIGIN      
        1 ttttcctcct gtcactagtg gcctgtcagg gcaggaaaaa caaaaaaaac cgaagagcat
       61 taccggattt caggaaaatt atcaacgcaa tatcaggcca atcaaaacag catcagaagc
      121 ccgactacgc ttctttgata aaatggtttc gaaagaaaac tctctggaag atgttgtttc
      181 tttaggtgaa atgattcaga aggaaattta tgggcatgaa caaagaacat tttcaccagt
      241 tcatcataca ggtaactgga aatcatcatt gttacacaac gcgctccttg gtctggcaaa
      301 tgtttataat ggcttacggg aaacagaata ccctaacact ttcaacagag atggtataaa
      361 aagtactaac tcttttagag ataacttatt gacaaaaaca agaactccca gagataattt
      421 tgaggaagga ataaaacatc ctgaacatgc aacaatacca tatgacaacg acaatgaaag
      481 taataaattg ctaaaagcag gaaagatagc tggtaacaat aacgagctgt tgatggaaat
      541 aaaaaaggaa tccaaaagcg accatcaaat ccccctgtca gataagttcc tgaaaaggaa
      601 aaaacggtc
//
LOCUS       DAZUGK010000578          608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZUGK010000578 DAZUGK010000000
VERSION     DAZUGK010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.582"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>608)
                     /locus_tag="ACHY5W_005179"
     CDS             complement(<1..>608)
                     /locus_tag="ACHY5W_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4449857.1"
                     /translation="YFTIESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKD
                     AFIHFYDYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETAL
                     DVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDM
                     TKGTQCMIHLMVNEEVFMSLPVPVATMVREDATNLINLKTLL"
BASE COUNT          202 a          118 c           89 g          199 t
ORIGIN      
        1 tgagtagtgt tttaagattg attaaattag ttgcatcttc acgtaccatc gtggcaacag
       61 gaacaggcaa tgacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc
      121 cctttgtcat atccacactt acgctacaaa tatcgttgta atgcgaagag tgcatttcag
      181 gaaacagatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat
      241 gaacaggttt tgtttcaaaa ttatcatcag caaatccaag cactattgca tgctctacat
      301 ctaacgcagt ttcaaatgac tcttcttctg taaaatcatt atttaaaacc tcacctgacg
      361 tattatcact gtcataaaaa gtgctcatca atgcgatatt gtttgttaca gcgtcttttg
      421 caatattctc attaattgca tatcgcctgc tataatcata aaaatgaata aacgcatcct
      481 ttataaagga tatatattgc tctttgttaa agtcaatacg actgtataat ttttcaaaaa
      541 tcacattcgc aatcttacta cccatagtat gcctgtataa taatgggtgc gattcaatcg
      601 taaaatac
//
LOCUS       DAZUGK010000579          604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZUGK010000579 DAZUGK010000000
VERSION     DAZUGK010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.583"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>604
                     /locus_tag="ACHY5W_005180"
     CDS             <1..>604
                     /locus_tag="ACHY5W_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4449858.1"
                     /translation="NNPEGERADLSKVVIEITLSAFTTMGTRQGHTAIIFQQPDGSTN
                     RYEGKSFERKDESSLHLITNKILACYQCEANKEIARLLNIPQELNNSQDLNNSQVSCK
                     DSVDSTITDLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVN
                     NYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVI"
BASE COUNT          215 a           99 c          112 g          178 t
ORIGIN      
        1 tgaataatcc tgaaggggaa cgagcggatt tatctaaggt ggtaatagaa atcacacttt
       61 ccgctttcac tactatgggg actcgtcaag gacatacggc tattatattt caacaacctg
      121 atggttcgac taatcgttat gaaggaaagt cctttgaaag aaaagatgag agttcattac
      181 acctgattac taacaagatt ctggcatgtt accaatgcga agctaacaag gaaatagctc
      241 gtctattaaa tattccgcag gagttaaata attctcagga cttgaataac tcgcaggtga
      301 gttgtaaaga ttcagttgat tctacgatta cggatttatt agaaaaacca ttgaacaatg
      361 cattattagc aataaggaaa gaacatctgc tattaatgcc ttatgtatgt aatgaatcga
      421 tttcatactt gcttggtgaa aaagggatac tgaaagaaat agatgacctt aacgcagtaa
      481 ataattatct tcttaataat aagaaagcta ctgacaatga aattaatgac atcaaagtta
      541 acctgtctca tattcttatt gattccttag atgatgcaaa agttaacctt acaccggtca
      601 tcga
//
LOCUS       DAZUGK010000580          603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZUGK010000580 DAZUGK010000000
VERSION     DAZUGK010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.584"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..512)
                     /locus_tag="ACHY5W_005181"
     CDS             complement(<1..512)
                     /locus_tag="ACHY5W_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFS4449859.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGS"
BASE COUNT          144 a          157 c          149 g          153 t
ORIGIN      
        1 gagccgctat gaaccagcag ttgcagatgc tgcgcatcca gacctgccag tgcaaacagt
       61 tcagcgttga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cacagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gcctgcggag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccggggtgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccatttatac tcgtcatact
      541 tcaagttgca tgaactgcga ctgcgttcgt tcaccccagt cacttactta tgtaagctcc
      601 tgg
//
LOCUS       DAZUGK010000581          601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZUGK010000581 DAZUGK010000000
VERSION     DAZUGK010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.585"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          172 a          140 c          146 g          143 t
ORIGIN      
        1 acggagtaaa actgataccg ccaaatgtca ccagaatatt gataacagag ggcgttgtag
       61 cggggttgtc acttaagcgt atggtcaacc tgacaacccg gtgtcctcaa ctggggaagg
      121 aataaccccg ccatacttac cgccgcgcca tttcgcggat tgccacaacc ggaagcgcac
      181 gttcgaagaa atctaacgac aagccttcta agggaaagag cttcgccgta cgctttcgcg
      241 ttgtgtgcct gcttttaacc acgtcaggcg aggtggtttc cgtcgttccc caacgacagg
      301 aaatctgtat aatctggata tccccaacaa caggaaggtg ttttatatgg tggttcaaaa
      361 gaattgttca ggtactggta agcctgcgga caggtcggac attccgaata ccaggaaaca
      421 tatacctgag aaaaacattc gaccaaaacc atctccagct ccttcagagg agagggggaa
      481 cagcaacaat caaacaagca ggtgatgata tatggaccgg gatgatattc ttgacagggt
      541 tttatatggt tattttcttg aacaactatt ctctgtagcg actggtcgtc tcgataaact
      601 t
//
LOCUS       DAZUGK010000582          601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZUGK010000582 DAZUGK010000000
VERSION     DAZUGK010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.586"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(108..320)
                     /gene="cspH"
                     /locus_tag="ACHY5W_005182"
     CDS             complement(108..320)
                     /gene="cspH"
                     /locus_tag="ACHY5W_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFS4449860.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          182 a          121 c          126 g          172 t
ORIGIN      
        1 taaatggatt agttttccgg tgaaaaagta acttgtgtta taatgcatgg tgttgactat
       61 tgaactatgc caataaaacc agtcagcttt aaacaagcag cgtcatatta agagagataa
      121 acatttgccg ctgttggtcc tcgcaggcca tttacgcggc aaaattccac gcgtaatcct
      181 ggtataagca cttctgcgtc gcggggagtg aatgcggaaa tatggacctg aacttcttta
      241 cgaccgtcgg aggggataat gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt
      301 cctgtcattt tacgggacaa acaaattcct tactgaaaat actgcgctgc actatacggg
      361 gttaataaaa taaagccagc gatatttaag accgccggac ggctaaaata aaatttgctt
      421 aatctcaatt atcatgcgtt aatagctgcg tcggtttgaa agacagacag catacaaagt
      481 agtttactaa agcagttctc attatcaggc attatcccct tcttttgagt ctctctcctg
      541 aacactaagt agtttctgta ttaaagccct gtttgccgaa aggcccaaaa tgaaggaagt
      601 a
//
LOCUS       DAZUGK010000583          601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZUGK010000583 DAZUGK010000000
VERSION     DAZUGK010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.587"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..523)
                     /locus_tag="ACHY5W_005183"
     CDS             complement(<1..523)
                     /locus_tag="ACHY5W_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS4449861.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGYVLSSLV"
BASE COUNT          187 a          124 c          103 g          187 t
ORIGIN      
        1 taaccagaga agataacaca taacctgatt gcactgagtt atctgtcagt ttcaggtatt
       61 ttgctttaaa cggaatagca tattgacctt ccgctgtttt ataactctca atgttaaaag
      121 gtgcagaaac atccaggaca tcgctgttat cacctaaata gagatagtaa cccacggtat
      181 cgttgctact ggctatcgcc ttatcgttac cggttatttt atcgttggta atcaacgaga
      241 gttttacacc agcaacttct ttcgggcagc taatttttaa catgctgctg gttacaccga
      301 ctgcatcatc ataggtagaa tatttagctt taccttcaaa gacactggtt ggtacagtcg
      361 gcatattaat acttactgaa tcaccacccg cttcgaattt acacggagat tctgcaatat
      421 tccccttaat agttaatgtt ccggtatcaa cgtctgcagc aaatactcca gcgctcataa
      481 aaaaagaaga aaagacagcg gcagcaataa ttgaacgttt cataatatgt tccttaataa
      541 aatttatttt tggttgttaa gagtctgtat ctcttggaaa caaatttctc acatcaaaaa
      601 a
//
LOCUS       DAZUGK010000584          599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZUGK010000584 DAZUGK010000000
VERSION     DAZUGK010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.588"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..338)
                     /locus_tag="ACHY5W_005184"
                     /pseudo
     CDS             complement(1..338)
                     /locus_tag="ACHY5W_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            430..599
                     /locus_tag="ACHY5W_005185"
                     /pseudo
     CDS             430..599
                     /locus_tag="ACHY5W_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          155 c          148 g          155 t
ORIGIN      
        1 gcgtgcgcta tccacttttt tgcccgtcca tattcaacgg cttctttgag gagttcattt
       61 tccatcgttt tcttgccgag caggcgctgg agttctttaa tctgcttcat ggcggcagca
      121 agttcagagg caggaacaac ctgttctccg gcggcgacag cagtaagact tccttcctag
      181 tattgcttac gccagagaaa taactggctg gctgctacac catgttgccg ggcaacgagg
      241 gagaccgtca tccccggttc aaagctctgc tgaacaattg cgatcttttc ctgtgtggta
      301 cgccgtctgc gtttctccgg ccctaagaca tcaatcatct gttctccaat gactagtcta
      361 aaaactagta ttaagactat cacttattta agtgatactg gttgtctgga gattcagggg
      421 gccagtctag tcatctacac aaccaacgcc atcgagtcgc tgaacagcgt aatccggcat
      481 gccatcaaaa aacgcaaggt gttcccgacg gatgactcag tgaaaaaggt cgtgtggctg
      541 gcaatccagg cggcttcaca gaaatggacg atgccactgc gggactggcg tatggcaat
//
LOCUS       DAZUGK010000585          598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZUGK010000585 DAZUGK010000000
VERSION     DAZUGK010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.589"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>598
                     /locus_tag="ACHY5W_005186"
     CDS             <1..>598
                     /locus_tag="ACHY5W_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449862.1"
                     /translation="SDKNALQKKLISRLNEIMNYAVNCGALKANPLLKIKTAFTGKKN
                     KSLAALPVERLPEFLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSG
                     LWIIPAHKMKCHREHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTP
                     IAASKFKGIVTLHGFRSMWSTLLNEEGFNPDVIEAALA"
BASE COUNT          194 a          102 c          133 g          169 t
ORIGIN      
        1 tatccgataa gaatgcgttg caaaaaaaat taatatcaag attaaatgaa attatgaatt
       61 atgctgtaaa ttgtggagca ttgaaagcaa atccattact taagataaag actgcattca
      121 caggaaagaa aaataaatca ttagcagcac tacctgttga aagattgcct gaatttctga
      181 gctggtggga tagtgtgcct catacctatc aaatagctca taatgcactt ttattccaga
      241 tattgacaat ggtcaggcca ggtgaggcga ttaaagcaga gtggtcagag attgattttg
      301 attctggctt gtggattatc cccgcgcata aaatgaaatg ccatcgtgaa catgttgttc
      361 ccttgtcatc acaggctatt agtatcctca gaacaatgca ggaaataaaa agagggcgtt
      421 atgtgttttt ttcctccaga acaaaagatg cgcctatggg gaggaatact atcaagaccc
      481 caattgctgc cagtaagttc aaagggattg taacgttaca tggttttcgt tcaatgtgga
      541 gtacgctttt aaatgaggag ggatttaacc ccgatgtaat cgaggctgca ttggcgca
//
LOCUS       DAZUGK010000586          591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZUGK010000586 DAZUGK010000000
VERSION     DAZUGK010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.590"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..75
                     /locus_tag="ACHY5W_005187"
     CDS             <1..75
                     /locus_tag="ACHY5W_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS4449863.1"
                     /translation="QIVVDEIRDTLEKAVIDCCDEADM"
     gene            72..>591
                     /locus_tag="ACHY5W_005188"
     CDS             72..>591
                     /locus_tag="ACHY5W_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS4449864.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRV"
BASE COUNT          127 a          151 c          173 g          140 t
ORIGIN      
        1 cagattgtgg ttgatgagat ccgcgacaca ctggaaaagg cagtcattga ctgttgtgat
       61 gaggccgata tgtgaatcag gtgaacgaga gccatagccg cgcatccgat atctggcgcg
      121 aagtggcctc gctgtttcgc ccacccagcc ggttaccagt agcggaagcc atcaggcgtt
      181 atatgcgggt tccacgggga gccaatactt ccggtccgtg ggagtcatcg ctgacgccct
      241 atatgattga ccccattaat acattatcag cccgtgaata tgacgcggtg gtgtttgtgg
      301 gacctgcgcg aaccgggaaa accgaagggc tgattgatgg ctggattgtg tacggcatca
      361 tctgtgatcc ggcggatatg ctggtggtgc agatgactga gacgaaggcg cgtgagcatt
      421 ccagaacgcg tctttccagg acgtttcgcc acagtccgga ggtcagcaag cgcctcagtc
      481 cttcccgtaa tgacaacaac gtccacgata aaatgtttct tgacggctcc ttcctgaaga
      541 ttggctggcc gtcgatcacc gtcttttcct cttcggatta ccgtcgtgtg g
//
LOCUS       DAZUGK010000587          590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZUGK010000587 DAZUGK010000000
VERSION     DAZUGK010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.591"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..442
                     /locus_tag="ACHY5W_005189"
     CDS             80..442
                     /locus_tag="ACHY5W_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFS4449865.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT          181 a          136 c          137 g          136 t
ORIGIN      
        1 gcaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct ataaatttat
       61 ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaaac gtctcagcga
      121 tcgtctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag ccgcagacaa
      181 atatgccgag ctggaaaaag agaaagccac gctggaagca gaaattgccc gcctgcgtga
      241 agttcacagc cagaaactga gtaaagaagc acagaagctg atgaagatgc cgtttcagcg
      301 cgcaattacc aaaaaagagc aggctgatat gggcaagctg aagaaaagtg ttcgcggact
      361 ggtggttgtt cacccaatga ccgcgctggg ccgcgaaatg ggcctggaag agatgactgg
      421 attctctaag accgcgtttt aagtccccta ctggtcaata caactaaagt ttcctcattt
      481 ttattcgctc ctgctggcat atgcaggagc atatttcaga tatcgaaatc tattaacatt
      541 atattttcaa ttcttcccac tcacctgaat gattttagca ggttaaaatc
//
LOCUS       DAZUGK010000588          587 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZUGK010000588 DAZUGK010000000
VERSION     DAZUGK010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.592"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(389..>587)
                     /locus_tag="ACHY5W_005190"
     CDS             complement(389..>587)
                     /locus_tag="ACHY5W_005190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4449866.1"
                     /translation="NGQQKYLNPGEIHKLHSAVFKVPHPERNHCLLLMGYLHGVQASE
                     LLGIKLSDIDLQAGNLNIRRL"
BASE COUNT          175 a          152 c          115 g          145 t
ORIGIN      
        1 ttcagaaaaa acagaatacc gcaatttcag taaacacagc gagagatatc cgcctcagta
       61 aaaaatcaga agattatcat cctgatttat tgcataaaac cagtcgagta gcggaatttt
      121 ctgcatccgg ataccactcc ccctgaaaaa tactcgcaca atatcgcgcc gcatgcactg
      181 atggaccggg ccatcgtgag aatctcttgc atcgtagata ttaaccacac cagtgccccc
      241 cagaacaaca gtaatacctg tagcagacct gccatagagg ttaaataacc attcatatcc
      301 ctcttctacc cattcatccc gaagtttcag ccagcgacga atggactgta cctcctgttt
      361 cagccgaggg gctgagttga caaaccgttt agagccgccg gatattcagg ttaccggcct
      421 gcaaatcaat atcagacagt tttataccta ataactctga tgcttgtaca ccatgaagat
      481 accccataag gagcaggcag tgattacgtt ccgggtgtgg cactttgaag acagcactgt
      541 gcaacttatg aatctcaccc gggttcagat atttttgttg tccatta
//
LOCUS       DAZUGK010000589          575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZUGK010000589 DAZUGK010000000
VERSION     DAZUGK010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.593"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>575
                     /locus_tag="ACHY5W_005191"
     rRNA            <1..>575
                     /locus_tag="ACHY5W_005191"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          126 c          171 g          124 t
ORIGIN      
        1 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
       61 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      121 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      181 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt
      241 gatggggtta gcggtaacgc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
      301 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
      361 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
      421 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
      481 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
      541 tggagccgac cttgaaatac caccctttaa tgttt
//
LOCUS       DAZUGK010000590          570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZUGK010000590 DAZUGK010000000
VERSION     DAZUGK010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.594"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            184..>570
                     /locus_tag="ACHY5W_005192"
     CDS             184..>570
                     /locus_tag="ACHY5W_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449867.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQYLL"
BASE COUNT          176 a          116 c          114 g          164 t
ORIGIN      
        1 tttcattagt aataatcact taaatgtatc gatgaaacag gtatgactta gcgggagtga
       61 ttgtttctga gtgtacagag accactttcg cctgataaat gaaaaatgag cgatttttgc
      121 aagttagttt ttctatagcc gagtacgctc agcgcctcgc ttgattacaa tgaggttgct
      181 gctatgagtc ctgataatat cggttcggct gtcaatcgct tctcaggttg gccatctaat
      241 gatcctctaa aaacagataa agaatcaatg acatcacccg tgactgtgca ggtaataaag
      301 atgattgccg ccatccttga cccacatcgg atagtcgttg gaatcgatac actttataca
      361 acgcagcgcg aaacatttac ctcagagagt caacagctca ttgaatcagc attgcaaaca
      421 cttcatgtga tgaatttaaa taacctggaa acaaatcgcg atgaagaagg tcactatctg
      481 gcttttgaat ataacgttga cacctctgac agaataaccg tttctgctta taatgaaaaa
      541 aagcctggct gctatggcca atatctttta
//
LOCUS       DAZUGK010000591          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZUGK010000591 DAZUGK010000000
VERSION     DAZUGK010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.595"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..268)
                     /locus_tag="ACHY5W_005193"
     CDS             complement(71..268)
                     /locus_tag="ACHY5W_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4449868.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
BASE COUNT          167 a          148 c          107 g          147 t
ORIGIN      
        1 acataagcaa aggactgaca ggtgccgcta acacccacca gccgcccatt taccacaaat
       61 aaaaaagcct tcaggactga aggcgtctgt aacaaccgca ctgatagtct gccagacccg
      121 ccataacaag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa gtattctgcg
      181 caatctcccc gactgtcgcc ggttcggtga cgcttaattc atcaaacaca actctggcgg
      241 tttctgtcat atcctgctgt ttcagcatgt ctttttaccc tttccggtta acgtgacaca
      301 ccaataactc ttgccgaaaa agccagcaag ctgaaagacc tgtattaata actaccagca
      361 catttaacgc actgcgctac tttgcgggca caaaaaaccc gctcagaggc ggggtcaagc
      421 tatgcggcga aataaccact cttaacagca tacctgattt tttacgtacg taaatgcttt
      481 gccgtgcata tttttcatgc aaatgtcacg tcctgctatt tttcagtctt ataaatttaa
      541 aaccatagaa aaaatcaatt atgttttaa
//
LOCUS       DAZUGK010000592          568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZUGK010000592 DAZUGK010000000
VERSION     DAZUGK010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.596"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..408
                     /locus_tag="ACHY5W_005194"
     CDS             <1..408
                     /locus_tag="ACHY5W_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS4449869.1"
                     /translation="KVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMSVL
                     ARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDGEI
                     RRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT          180 a           96 c          154 g          138 t
ORIGIN      
        1 aaagtattta cacaggaaga gcgggaaaaa attaaagggc aggttgttga gctagtacgc
       61 cggagtgggc gcgagacgtt acggcaactg gaagccaaga caggtgcgac aagatatctg
      121 atgagcgttc ttgccagaga gctggttgcc agtggcgatg tatacaactc tggttacggg
      181 ttattcccat ctgaacaggc tcgtaaggac tggcaaaacg cccgcaaaaa actatcgagg
      241 gcaaaactga agaaaccggt tgtggttgat cctgacctta tctggtcatt accagacgga
      301 gaaatacgcc gctatgacag gcaattgaac ataatttgta gtgagtgccg taacagtgaa
      361 gtgatgcagc gagttttgat attttacaca ggagtaatga tggaatagtg aaaataacat
      421 gaaacctttg gattctggtg tttcagtgga tgggaaaaga gcagatacat agaaaatgaa
      481 tagcaataat tcacagtctg gatgttgttt gtatgcttat aaaatgatcc tagtatgcat
      541 tgccggagtt gaatcagctc caatgaag
//
LOCUS       DAZUGK010000593          565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZUGK010000593 DAZUGK010000000
VERSION     DAZUGK010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.598"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..419
                     /gene="traT"
                     /locus_tag="ACHY5W_005195"
     CDS             <1..419
                     /gene="traT"
                     /locus_tag="ACHY5W_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFS4449870.1"
                     /translation="YEINVYPTYLIPLFLPVSFNILEKPAEKIYRKGGTTLGVGLATG
                     LIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQH
                     KYQTRVVSSANQANLKFEEAKPHLEDQLAKSIANIL"
     gene            complement(467..>565)
                     /locus_tag="ACHY5W_005196"
     CDS             complement(467..>565)
                     /locus_tag="ACHY5W_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS4449871.1"
                     /translation="RTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          169 a          143 c          117 g          136 t
ORIGIN      
        1 cttatgaaat aaacgtttac ccgacgtatc tgatcccgct gtttttacct gtatccttta
       61 acatacttga gaagcccgct gaaaaaatat accgtaaagg cggtaccact ctcggtgttg
      121 gactggctac cggtctgatt ggcatggccg cggatgcaat ggtggaagat ataaattaca
      181 caatgattac agatgttcag attgccgagc gaaccagaac cagcgtcaga acggataatg
      241 ttgcagcttt gcgtcaggga acatcaggct ccaaaattca gacaagcaca gaaaccggca
      301 accagcataa ataccagacc cgggttgtat ccagtgccaa ccaggctaat ctgaagtttg
      361 aagaagctaa acctcatctg gaagaccagc tggcaaaatc aatcgccaat atactctgat
      421 ataaatatcc cggaggccat tcacctggcc tccggccctc ttagtctcaa cgttcagaca
      481 attcagatat tattttccct gtcacaccat atacccatgc cggaactgaa tctaatgacg
      541 gtaccagtaa attcttcagc gttcg
//
LOCUS       DAZUGK010000594          563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZUGK010000594 DAZUGK010000000
VERSION     DAZUGK010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.599"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>563
                     /gene="ehxD"
                     /locus_tag="ACHY5W_005197"
     CDS             <1..>563
                     /gene="ehxD"
                     /locus_tag="ACHY5W_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFS4449872.1"
                     /translation="AKNEHAVWLAQVSQLEKEIELVREELALETNIFRSEIIEKHRKS
                     TDNIVLLEHELEKNRQRKASSFIKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDI
                     LEVTASVLNKDIGFIQPGQEVVIKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFN
                     VIISVDRNDIQGERKKIPVTAGMTVMA"
BASE COUNT          196 a           89 c          128 g          150 t
ORIGIN      
        1 ggcaaaaaat gaacatgcag tctggcttgc acaggtttct caacttgaaa aagaaataga
       61 acttgtgcgg gaagaactgg cactggagac gaatatcttc agaagtgaaa ttatcgagaa
      121 gcacagaaaa tcaacagata acattgtgtt gctggagcat gagcttgaaa aaaacaggca
      181 gagaaaagca tcatctttta ttaaagctcc tgtgagtggt actgttcagg agttaaatat
      241 acatacagaa ggtggtgttg tcacaacagc agaaacgctg atgattattg tccctgataa
      301 tgatattctc gaagtaacag catctgtact taacaaggat atcggtttta tacaacctgg
      361 acaggaggtc gttattaaag tagatgcata tccatataca cgtcatggtt accttacagg
      421 gaaagtaaaa aatataactg cagattctgt ttctgttccg gatacagggc ttgtatttaa
      481 cgtgattata tctgttgatc ggaatgatat acagggagaa agaaaaaaaa tccctgttac
      541 agctggaatg actgttatgg cag
//
LOCUS       DAZUGK010000595          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZUGK010000595 DAZUGK010000000
VERSION     DAZUGK010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.600"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            177..>556
                     /locus_tag="ACHY5W_005198"
     CDS             177..>556
                     /locus_tag="ACHY5W_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFS4449873.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWT"
BASE COUNT          143 a          104 c          143 g          166 t
ORIGIN      
        1 catttttgtg attttatcgt catcatcatc atgatggtaa cttgttgttt ttaatgctgt
       61 tagtattaaa aagatgatga ttgtggttgt tttttgttcg acatctttat atggcggcat
      121 ttctttacaa aaaaacagag tcacttctgt tctgcggttt atgtggaggg atgtaaatgg
      181 acggcgagct gaaaaatatg aagttaaata ttaatcaact ggcagccctt tcaggtctgc
      241 accggcagac tgttgccgcc agaatggcgg atgttcctct tgcaccaggc agtaatgaaa
      301 agaaaaaact gtatctcctg acggatttga taacttcgtt gctggaaaaa ccaccatctt
      361 ccgaagatga ggatatggat cctcacgctc gtaaggcatg gtatcagtcc gagcgcgagc
      421 gtcttaaatt tcagcatgaa actgttcagc ttgtgccagt cagtgatgtc aggcggtcct
      481 tttctgtcgt ggtgaaagcg atagttcagg tactggaaac ctggcctgac cggctggaga
      541 gggacagggg gtggac
//
LOCUS       DAZUGK010000596          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZUGK010000596 DAZUGK010000000
VERSION     DAZUGK010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.601"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..335)
                     /locus_tag="ACHY5W_005199"
                     /pseudo
     CDS             complement(1..335)
                     /locus_tag="ACHY5W_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          140 a          136 c          125 g          155 t
ORIGIN      
        1 gattctggaa aaggactttg tggattgttg ctattgccag tgagattgaa tgaagtcgtt
       61 ttaactcgct ttgaatacgt cttgccccca gatttcgctg agaacgtaac tccagaatca
      121 gagcgacttc gcaagtgccg gttttcgttg atggtgaacg cttggggcga cggctgtggc
      181 tttccagacc agcgatgccc tgagccagat atctgcgcca ccattttacg caatgtgggc
      241 cgagaaatgc cacagcgacg gcatacaaat cccgcatcac cggacgtttc atagagtttt
      301 acccactgta gccgctgctg aatttccctg ttcataattt ggcattagtt cagctcaatc
      361 ccgacttaaa aagtccctac aggatgttcc ggacctgagg cacagttcat atccttatcc
      421 ataaaaaatt tagctatgtc tatagcatta atcttgacaa gacgacgtta gggcctggcg
      481 atgaatcatt tcccctgcca gatatccttt ggggtgcacc agaactaccg ttttatatct
      541 atataactga aacgtc
//
LOCUS       DAZUGK010000597          551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZUGK010000597 DAZUGK010000000
VERSION     DAZUGK010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.602"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..182
                     /locus_tag="ACHY5W_005200"
     CDS             <1..182
                     /locus_tag="ACHY5W_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFS4449874.1"
                     /translation="SLAESIRQEISTFSGMNRIYLTGGGAELIYPHIKQYFPNLKVNK
                     VDEPQFALVKAMVHA"
     gene            182..>551
                     /locus_tag="ACHY5W_005201"
     CDS             182..>551
                     /locus_tag="ACHY5W_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFS4449875.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTR"
BASE COUNT          195 a          117 c          111 g          128 t
ORIGIN      
        1 aatctctggc agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct
       61 atctcactgg cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc
      121 tgaaagtgaa caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt
      181 aatggaaagc agtgatccga aaaaaagaaa gaaagttgtg gcatacctgc acccgacact
      241 ttatccgcag gataacctga cacaacaaac aatagactct ctcccgatac aaatgcgggg
      301 agacttctac agacaatctc ttatttgcgg agcagctctt tattctgttg acccccgact
      361 gttaacgcta atcagtggtt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact
      421 tatagagcaa acaacaggat acacatctac atcaattgat ataagtgtgt taaaaaacat
      481 cataggggca tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga
      541 acaaaccaga c
//
LOCUS       DAZUGK010000598          543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZUGK010000598 DAZUGK010000000
VERSION     DAZUGK010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.603"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..543
                     /locus_tag="ACHY5W_005202"
                     /pseudo
     CDS             1..543
                     /locus_tag="ACHY5W_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058103.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          145 a          126 c          119 g          153 t
ORIGIN      
        1 actgcattat cgccaacgcc ccgacacgta attcctgagc aattagaaaa aacagttcgt
       61 tatcttacac aaatgtgcat ccacgtaatg ccgacaatat tgataatctg aataggtccg
      121 tagagtatat aaaagaggtc tttgtcagta gcggtgccag agttaccgcg cagggcgtcc
      181 ccgattggct attacgactc tgcgcctggt agccaggatt acccttaccc gcaatgtcct
      241 ggctttatcc cgatcgggga gactttattg ccgtggtcgg cagaatgcag gatatcaacg
      301 ccgttcgtca ggtaaaagcg gcattgttgt catctcagga tttatctgtt tattctatga
      361 ataccccagg gtttattccc ggtattgatt tctctgatca cctgaattat tggcaacacg
      421 atattcccgc cataatgatt actgacaccg ctttttatcg taataaacaa taccacttgc
      481 ccggtgatac cgcagacaga ttgaattatc agaaaatggc tcaggtagtg gatggtgtta
      541 taa
//
LOCUS       DAZUGK010000599          542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZUGK010000599 DAZUGK010000000
VERSION     DAZUGK010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.604"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            252..>542
                     /locus_tag="ACHY5W_005203"
     CDS             252..>542
                     /locus_tag="ACHY5W_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS4449876.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAVPRDEK"
BASE COUNT          167 a           95 c          110 g          170 t
ORIGIN      
        1 aataaagtgt gcagtcgttt cattaccgta tatctttagt agttctaata aactatcaaa
       61 aatcgctcat ttctgcttat tgatagaaag attgattgcc agcctgttta gatccgtaca
      121 gcagcagact ttcataaccc ggttgatcct gagtttgcag tgcgaaaagt taaaaatgag
      181 cgatttctgt aagattcccc attttgccag gcttatgatt cgcagatcat tcaagtgcaa
      241 aggagcgatg tatggattgt tcaaaatgca atggttatgc gacgatgtta ttaaatatgg
      301 tgcagggttc tgacccggtt aatttactgg aattacatgg tttccttgaa catttcgcat
      361 attatgtctc atttggcaaa tttaatgctg gccaccagcg ctataatgca tttaaaaaat
      421 tcgttagtga gatttcggag atcagcgcaa acgatatcaa tatgacgatt aaaaccgggc
      481 aatccagaca tgaaaatgtt atctctataa atatgaacga tgcagttcct cgtgatgaga
      541 aa
//
LOCUS       DAZUGK010000600          533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZUGK010000600 DAZUGK010000000
VERSION     DAZUGK010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.605"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>533
                     /locus_tag="ACHY5W_005204"
     rRNA            <1..>533
                     /locus_tag="ACHY5W_005204"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          133 a          125 c          162 g          113 t
ORIGIN      
        1 gaagttttca gagatgagaa tgtgccttcg ggaaccgtga gacaggtgct gcatggctgt
       61 cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt
      121 tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac tggaggaagg
      181 tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg
      241 cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc
      301 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gtggatcaga
      361 atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg
      421 gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt gtgattcatg
      481 actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat cac
//
LOCUS       DAZUGK010000601          530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZUGK010000601 DAZUGK010000000
VERSION     DAZUGK010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.606"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>530
                     /locus_tag="ACHY5W_005205"
     rRNA            <1..>530
                     /locus_tag="ACHY5W_005205"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a          119 c          176 g          112 t
ORIGIN      
        1 tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt ggctaatcct
       61 ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga gcgtgacggc
      121 gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa gggccatcgc
      181 tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag ttcatatcga
      241 cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag taggtcccaa
      301 gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg tcgtgagaca
      361 gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc tagtacgaga
      421 ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac tgcccggtag
      481 ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
//
LOCUS       DAZUGK010000602          525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZUGK010000602 DAZUGK010000000
VERSION     DAZUGK010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.607"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(210..284)
                     /locus_tag="ACHY5W_005206"
                     /pseudo
     CDS             complement(210..284)
                     /locus_tag="ACHY5W_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          168 a           97 c          108 g          152 t
ORIGIN      
        1 atggtggaaa ctattaagaa aattaattct gagcataaca tggcgaataa ataaacggat
       61 caagttgttc ctgtgacaat gaggttatac gaaaagacgc ttactcatca gaaatctggt
      121 atgttttaat taaaccagtg tcgctgatgt gtaagcgttg acgttgggag acgattaaat
      181 ggttatacca ctcctacgtc ttttccatac ggcgtataag agtgcaacat gttgagtttt
      241 ccgagtgaat ggtgcatttt actaaccgag ccatttgaat caaccaacgc aataaaaaag
      301 actggaataa taagcctgta tttattattc atcgcaatcg cactctacgg ttttatcctg
      361 cagaaatgag actacttcca gcggttgtgg attatccctg ggcgatagtc atagtctggt
      421 gtcaggattc acaaatgacc tgttacctga catggatata aacacaacat agagtgcggt
      481 agttaatctg aatcatctat ttttcggcta cacagcacaa aacaa
//
LOCUS       DAZUGK010000603          522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZUGK010000603 DAZUGK010000000
VERSION     DAZUGK010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.608"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..>522
                     /locus_tag="ACHY5W_005207"
     CDS             68..>522
                     /locus_tag="ACHY5W_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449877.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVE"
BASE COUNT          197 a          113 c           84 g          128 t
ORIGIN      
        1 tccattcctg ctttaattat ccaatttaaa aatcttagca taaaacagat aatgaataag
       61 gaatgttatg tcaattgact ttaccccagg tatgataaat acatatcacg gcgatattta
      121 taaccgcaca acagataccg acaatgttaa aacaccagac acaccgatgt ggccttgtga
      181 taatcgggaa gaacaacaac ccattaattc cacattctct ggagaaggat acaaccctga
      241 acaatatgat ttagcgcggc aacacctccc acaaatttat gcgtgccgca cgaacaccaa
      301 atatacagat gcagactact caaaagttgt ggctcaactt gtaagtctta ttactaacat
      361 tgaatcgata agttcgacac cacttacgcg gcaggcacag gaaatactta atcaaattaa
      421 taacattaga tacgaaaaga acaaaagtgc tgaatgtcga atcattgttg tcgctaatcc
      481 taaacccgac aaggcaatca taactaaaat tagcgtagaa ga
//
LOCUS       DAZUGK010000604          521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZUGK010000604 DAZUGK010000000
VERSION     DAZUGK010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.609"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHY5W_005208"
     CDS             complement(<1..278)
                     /locus_tag="ACHY5W_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4449878.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTTTGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWP"
     gene            complement(315..>521)
                     /locus_tag="ACHY5W_005209"
     CDS             complement(315..>521)
                     /locus_tag="ACHY5W_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449879.1"
                     /translation="LAACPGMTVESARAVLAAGSGKAEPVSASTTALFERIMANHSPA
                     AVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT           99 a          163 c          137 g          122 t
ORIGIN      
        1 tcaggccagc ggattgcctc cgtcgcaaag gtccccgact tgtaatacgt cagtaccgtc
       61 tctgtgcctt caagcggcag taccagtatg ccaaccgcac taccggcttt ctgtccgtcc
      121 cagaccacca gtttcccggt agcttcatcc agcatcaggg gcgtcagtgc cggtgttgcc
      181 gaggaaatcc cgctgctgcc tgtggtggta tgagccggat cattaccggc aaaaatacgt
      241 acttccgcac gctgttcagt gatggttttc gttaccatat tgtaaaaacc tcctgttgat
      301 ggtcagcact gacttcatgg catggccatg agcattttca cgtccgcatc accgtctgct
      361 gacgtctgtg gcacgccacc ctgtaccgct gccggtgaat ggttcgccat gatgcgttca
      421 aacagggcgg ttgtggatgc agagaccggt tctgccttac ctgatcccgc agccagcaca
      481 gcccgggcgc tctccacagt cattcccggg caggcagcca g
//
LOCUS       DAZUGK010000605          521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZUGK010000605 DAZUGK010000000
VERSION     DAZUGK010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.610"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHY5W_005210"
     CDS             complement(<1..278)
                     /locus_tag="ACHY5W_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS4449880.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWP"
     gene            complement(315..>521)
                     /locus_tag="ACHY5W_005211"
     CDS             complement(315..>521)
                     /locus_tag="ACHY5W_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449881.1"
                     /translation="LAACPGMTVESARAVLAAGSGKAEPVSASTTALFERIMANHSPA
                     AVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT           99 a          163 c          137 g          122 t
ORIGIN      
        1 tcaggccagc ggattgcctc cgtcgcaaag gtccccgact tgtaataggt cagtaccgtc
       61 tctgtgcctt caagcggcag taccagtatg ccaactgcac taccggcttt ctgtccgtcc
      121 cagaccacca gtttcccgct ggcttcatcc agcatcaggg gcgtcagtgc cggtgttgcc
      181 gaggaaatcc cgctgctgcc tgtggcggta tgagccggat cattaccggc aaaaatacgt
      241 acttccgcac gctgttcagt gatggttttc gttaccatat tgtaaaaacc tcctgttgat
      301 gatcagcact gacttcatgg catggccatg agcattttca cgtccgcatc accgtctgct
      361 gacgtctgtg gcacgccacc ctgtaccgct gccggtgaat ggttcgccat gatgcgttca
      421 aacagggcgg ttgtggatgc agagaccggt tctgccttac ctgatcccgc agccagcaca
      481 gcccgggcgc tctccacagt cattcccggg caggcagcca g
//
LOCUS       DAZUGK010000606          512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZUGK010000606 DAZUGK010000000
VERSION     DAZUGK010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.611"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(123..>512)
                     /locus_tag="ACHY5W_005212"
     CDS             complement(123..>512)
                     /locus_tag="ACHY5W_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS4449882.1"
                     /translation="KPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVRRC
                     LVDVLACGDLYMPGKYGFFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQNI
                     ICGECRKSEVMLRVLAFYQGNFQEAIL"
BASE COUNT          115 a          135 c          100 g          162 t
ORIGIN      
        1 gggtcatcct cttttaccgg aaataaaacg atattgctta actcatcttc tggttttatt
       61 tccatgctct tctcctttga tgtgaatgcc agcggtaatt gaagcctgat agctaatttc
      121 attcacagta tcgcctcctg aaaattaccc tgatagaaag ccagtacacg cagcataact
      181 tcactcttcc ggcactcgcc acagattatg ttctgttgtc tgtcgtagcg gcgtatttct
      241 ccgtctggta actttcgaat caatgtctgg tcggttgttt tctccgctgc cttacgccat
      301 acgcgataca cctgttctga tgtaaaaaaa ccgtatttac cgggcatgta taaatcgcca
      361 caagccagta catccacaag gcaacgtctg actgaatgcc agcctgctcc cgtcgctctc
      421 tccagttgtg atatcgtcat gcgttcattt ttgcgtacca gcccgataat tcgggccttc
      481 agttcttcac gctgttcgtg tgtaaaaggt tt
//
LOCUS       DAZUGK010000607          510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZUGK010000607 DAZUGK010000000
VERSION     DAZUGK010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.612"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..185)
                     /locus_tag="ACHY5W_005213"
     CDS             complement(<1..185)
                     /locus_tag="ACHY5W_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS4449883.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRS"
     gene            complement(192..>510)
                     /locus_tag="ACHY5W_005214"
     CDS             complement(192..>510)
                     /locus_tag="ACHY5W_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFS4449884.1"
                     /translation="RGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRTEQGANG
                     AIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPG
                     FRG"
BASE COUNT          113 a          150 c          124 g          123 t
ORIGIN      
        1 gagcgattca gaattttttc cacccgcaac tgacgattca ggcggttgat ctcctcgcaa
       61 cgtttctgtc cttcagcaag ttgccactca cgccactccg caaccgttct gaatggggca
      121 gttacatgtg gtggggtcag tctgcggata cgctccagaa cgccgcctgt cgcaatattt
      181 ttcatggtct gttaccccct gaagcctggc gggatcgcac tatccggtag cgaaacggtg
      241 ttaacctgtc ggagtaacgt ctcaggtcga acacctttcg gcgcgaacaa gccctggtat
      301 tcattggcga tgctgtgtcg aatcacctgc tcaggtgaaa aaccctgctg gcggaatttt
      361 tccagctccc gtatcgcccc gttagcgccc tgctccgttc gaatcggttt acgcaatgcc
      421 tggcgaaatt caacccactc acgccaaagc gagacagaaa tccagttcgg caaagcaata
      481 tccagagggt caaacttttt gacacctcga
//
LOCUS       DAZUGK010000608          503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZUGK010000608 DAZUGK010000000
VERSION     DAZUGK010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.613"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..484
                     /locus_tag="ACHY5W_005215"
     CDS             <1..484
                     /locus_tag="ACHY5W_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4449885.1"
                     /translation="KTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGHSISQYR
                     LGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILW
                     YKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH
                     "
BASE COUNT          154 a          117 c          113 g          119 t
ORIGIN      
        1 gaaaacaacg ttgcgtgata aagccatggt gaattatgcc tttgattatt taagctcacc
       61 gggcagtctt ccattcacca cggcagccac ggagctttcc gcgattcatg gtcactcaat
      121 atcgcaatat cgccttggag aattttatct tcatggtagc gacggtaaac cgctggatta
      181 tacacaggcg agatactggt atgagcaatc agcggaacag gaaaatccac gcgcgcaaag
      241 taaactgggg tggatctacc tcaaaggtct gggggtcaaa cccgacaccc gtaaagcaat
      301 tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctctaggttt
      361 gatctacaga aatggctcag gtattaatgt taaccattat gaatctcaaa aatggttaaa
      421 actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccgggc ttcccgcaca
      481 ttaacgtcga ctgtcacccg aac
//
LOCUS       DAZUGK010000609          502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZUGK010000609 DAZUGK010000000
VERSION     DAZUGK010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.614"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..367
                     /locus_tag="ACHY5W_005216"
     CDS             2..367
                     /locus_tag="ACHY5W_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449886.1"
                     /translation="MRFTIWRELNNIIPTAKDYRFDIMVYDRIDTGFMGCIGHHKENY
                     LTISYTLWLAKDVLWADIICQNRSEYVRIFTQTNERYRAKDNRAFVYYHWSFKHWTFI
                     DSYREQQEKINGKHIQSDK"
BASE COUNT          176 a           75 c          106 g          145 t
ORIGIN      
        1 gatgcggttt acaatatgga gggaacttaa taacatcatt cccactgcga aagattacag
       61 gtttgatata atggtatatg atcgcattga cactggtttt atggggtgta ttggacatca
      121 taaggagaac taccttacta tctcttatac attatggtta gcaaaagatg tgctatgggc
      181 tgatattatt tgtcaaaaca gaagtgaata tgtgcgcata ttcactcaga cgaatgaaag
      241 atatagagca aaagataata gagcttttgt gtattatcac tggagtttca agcactggac
      301 ctttatcgat tcatatcgtg aacaacagga gaaaattaat ggcaaacata tacagtcaga
      361 caaatagtga tggtaaatta gaaaaatgga cccttcggct aatagtgtca gcaggagaac
      421 accccttacc taaaatttct gatatcgggg ttgcaagttt aaaaatacat tattgtctct
      481 ggaatgaaga ggaatacaat ga
//
LOCUS       DAZUGK010000610          499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZUGK010000610 DAZUGK010000000
VERSION     DAZUGK010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.615"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..226
                     /locus_tag="ACHY5W_005217"
     CDS             <1..226
                     /locus_tag="ACHY5W_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449887.1"
                     /translation="FMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAP
                     SPIIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            293..472
                     /locus_tag="ACHY5W_005218"
     CDS             293..472
                     /locus_tag="ACHY5W_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFS4449888.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT          167 a           90 c          103 g          139 t
ORIGIN      
        1 atttatgaat agatcattgg cccaaataac aggcgagaat atgattggtg caaatggcag
       61 gagcgtacca gaaatggcac ttcccgaaag ctataactat atccacaagt cgggaacttt
      121 gcatgaagcg ccaagcccta tcattccgct aaattggtcg aaagcttcaa tgactctcat
      181 gttgaaagag atgtccaacc ttatcaatga tgagggaatt aaatagtttt cataatgagg
      241 cggtcctgga ttaaaaggac cgctcaaaga gaaatcgaaa ggggaggtaa aagtgatcaa
      301 taaattactg ttagcttacc tgataggtct ggttgtgaca tcgactttaa tatttatctt
      361 ctcggaagaa aaagtaacat atcggttgtt tgctgcaata attacaggtt taacctggcc
      421 gcttagtctc attccttcca ttataagtct catgattcgt aaatccgact aacaagaaaa
      481 ttatatgatc gattaagaa
//
LOCUS       DAZUGK010000611          495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZUGK010000611 DAZUGK010000000
VERSION     DAZUGK010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.616"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..>495
                     /locus_tag="ACHY5W_005219"
     CDS             119..>495
                     /locus_tag="ACHY5W_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4449889.1"
                     /translation="MDISLLSTTSDPEQLRALAIAMVQKVMAENAELQNRIRILEEQM
                     KLARQQRFGKKCESLAGMQRSLFEEDVDADIAEISAHLDKLLPQTGDEEKTTTRPVRK
                     PLPSPLPRAEKVIPPAEERCPDC"
BASE COUNT          142 a          129 c          122 g          102 t
ORIGIN      
        1 gaaagggcat gacctggcaa aatgggtata ccaggaagaa cccgaagatg ctcagagcaa
       61 cgcaataact caatgaaata taatgaatat ccatgcgtga tgtcatataa tccaccgcat
      121 ggatatctcg cttctcagta ccaccagtga ccctgaacag cttcgcgctc tggcaattgc
      181 catggtgcaa aaagtcatgg ctgagaatgc agaacttcag aaccgtatcc gcatcctgga
      241 agagcagatg aagctcgcac gccagcagcg tttcggtaaa aaatgcgaat cgctggccgg
      301 gatgcagcgt tccctgttcg aagaggatgt tgacgcggat atcgcagaaa tctcggcgca
      361 tctggacaaa ctgcttcctc agaccgggga cgaagaaaaa acaacaacac gtcctgttcg
      421 taagcccttg ccttcacctc ttccccgtgc tgaaaaggtt atcccgccag ctgaggaacg
      481 ttgtcctgac tgtga
//
LOCUS       DAZUGK010000612          495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZUGK010000612 DAZUGK010000000
VERSION     DAZUGK010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.617"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..478
                     /locus_tag="ACHY5W_005220"
     CDS             <1..478
                     /locus_tag="ACHY5W_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFS4449890.1"
                     /translation="EQGMPVLRGGGKGNEVLYDSAAVIKWYAERDAEIENEKLRREVE
                     ELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAFCTFVLSRIAGEI
                     ASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT          129 a          113 c          152 g          101 t
ORIGIN      
        1 ggaacaggga atgcccgttc tgcgaggcgg tggcaagggt aatgaggtgc tttatgattc
       61 tgccgccgtc ataaaatggt atgccgaaag ggatgctgaa attgagaacg aaaagctgcg
      121 ccgggaagtt gaagaactgc ggcaggccag cgagacagat ctccagccag ggactattga
      181 gtacgaacgc catcgactta cgcgtgcgca ggccgacgca caggagctga aaaatgccag
      241 agactccgct gaagtggtgg aaaccgcatt ctgtactttc gtgctgtcgc ggatcgcagg
      301 tgaaattgcc agtattctcg acgggatccc cctgtcggtg cagcggcgtt ttccggaact
      361 ggaaaaccga catgttgatt tcctgaaacg ggatatcatc aaagccatga acaaagcagc
      421 cgcgctggat gaactgatac cggggttgct gagtgaatat atcgaacagt caggttaaca
      481 ggctgcggca ttttg
//
LOCUS       DAZUGK010000613          493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZUGK010000613 DAZUGK010000000
VERSION     DAZUGK010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.618"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            165..240
                     /locus_tag="ACHY5W_005221"
     tRNA            165..240
                     /locus_tag="ACHY5W_005221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Ile,seq:cat)
BASE COUNT          136 a           88 c          126 g          143 t
ORIGIN      
        1 ttgttgacta cgagtatcga ctttgcgaat ggaggcgatg tcgggtttta tgtcgcatgt
       61 attgacgacc caggatggat gagaacgcat aaaaatctgt tatttacatc ataagatcat
      121 catgtttatt gcgtggttta taagtattgt tattgcatac ttaaggccct ttagctcagt
      181 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      241 aatcatcatt catgtatcag gaagagttaa gggtacaagg ggtactcgat ggcggtctat
      301 atttcatgac taacgccatt taaaggaaga agtgcggtgg gtggtttcga atgagggctg
      361 gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc attcaaaagt
      421 acctggaaaa aatgcccgct atgccaagcg ggcaaattgc ttacatgtgg aattaagtaa
      481 ttaacgataa agc
//
LOCUS       DAZUGK010000614          482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZUGK010000614 DAZUGK010000000
VERSION     DAZUGK010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.619"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>482
                     /locus_tag="ACHY5W_005222"
     CDS             <1..>482
                     /locus_tag="ACHY5W_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS4449891.1"
                     /translation="QAKWDTPRVVKGVRFSLRLTSGSGENSRLVTTAITADTAHRFSG
                     LPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDP
                     TVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKS
                     A"
BASE COUNT          106 a          139 c          146 g           91 t
ORIGIN      
        1 caggcgaaat gggacacgcc gcgggtggtg aagggtgtgc gcttcagtct gcgcctgacc
       61 agtggaagcg gtgaaaacag ccgcctggtg accaccgcca tcaccgcaga cacggcgcac
      121 cgtttcagtg gcctgccgct cggggaatac accctgacag tcagggcaat taacagttat
      181 ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc aaaacccgcc
      241 accattgaac tgacgccggg gtattttcag ataacggcgg tcccgcgtct tgcggtgtat
      301 gacccgacgg tacagtttga gttctggttt tcggagacaa aaatcgcaga cacatctcag
      361 gtggaaacct ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc cggcccgcac
      421 attaagcccg ggaaggattt ctggttttac gtgcgcagcg tcaacctggt ggggaaatct
      481 gc
//
LOCUS       DAZUGK010000615          480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZUGK010000615 DAZUGK010000000
VERSION     DAZUGK010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.621"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..>480
                     /gene="nleB2"
                     /locus_tag="ACHY5W_005223"
     CDS             134..>480
                     /gene="nleB2"
                     /locus_tag="ACHY5W_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFS4449892.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYR"
BASE COUNT          163 a           66 c          107 g          144 t
ORIGIN      
        1 gtcatgtccg gtggatgttt gttaggaaag caaagatggc aaaactgctg gaggttttgt
       61 ggttgagtat gccaatataa ttaatagatt aaagagttag ttgtgaagaa aatatggata
      121 aacaggacga cgaatgcttt caccgataag gacaactttc cataactcag taaatatagt
      181 gcagagttca ccctgtcaaa cggtttcttt tgcaggaaag gaatatgagt taaaggtcat
      241 tgatgaaaaa acgcctattc tttttcagtg gtttgaacct aatcctgaac gatataagaa
      301 agatgaggtt ccaatagtta atactaagca gcatccctat ttagataatg tcacaaatgc
      361 ggcaaggata gagagtgatc gtatgatagg tatttttgtt gatggcgatt tttcagtcaa
      421 ccaaaagact gctttttcaa aattggaacg agattttgaa aatgtaatga taatctatcg
//
LOCUS       DAZUGK010000616          475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZUGK010000616 DAZUGK010000000
VERSION     DAZUGK010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.622"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>475
                     /locus_tag="ACHY5W_005224"
     CDS             <1..>475
                     /locus_tag="ACHY5W_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFS4449893.1"
                     /translation="SHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSLQS
                     HITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSGKR
                     KEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR"
BASE COUNT          144 a          110 c          133 g           88 t
ORIGIN      
        1 tcacattcca gagctgcggc actggcggtt gactctgccg ccgcaaaact gatgcagaat
       61 gcagaaaata tcagtgaata cctgttgtta cgccatcagg ggctacagca aagcattcag
      121 tcactgcagt cgcatatcac cagcctgctt gcggatatcc gggaaatcgc ggactgtcag
      181 gagcagatga ccactgatgt cagaatggcc atggaggaga tcgatactaa aacccgggaa
      241 ttactgacgg gggtctgcac ctcactggaa gaggagctga atgactattt cagaagcggt
      301 aaacgcaaag aacagcaaat gctggaggaa gaagacgcag aacaacgaag gtctcagtcc
      361 ggattatggg ggaaaatttc tcaatggtcc ggtatcaaca accagggccg ggaggattac
      421 aggaaacgag attttgcccc ggacagcccc gaaataaaat tcagtgatcg caggg
//
LOCUS       DAZUGK010000617          469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZUGK010000617 DAZUGK010000000
VERSION     DAZUGK010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.623"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>469
                     /locus_tag="ACHY5W_005225"
     CDS             <1..>469
                     /locus_tag="ACHY5W_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFS4449894.1"
                     /translation="YALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVTLSTL
                     PGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFSSLSR
                     DGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL"
BASE COUNT          136 a           86 c           99 g          148 t
ORIGIN      
        1 tacgcgctta aatatagctc ccttactggt gggcttatag ctgtgtcaga attaagtaag
       61 aaggtcacag gaaaaaccgg cagaagatta atgacggttt ccctggtatt atcagtgact
      121 ctttctactt taccgggtaa agcatcaacg gtcagcgcag aaataccata tcagactttt
      181 cgtgactttg ctgaaaataa aggtgtgttt acccccggag tgacaggaat tgaaataaac
      241 gacaacaatg gaaataaagt tggggttctt gatgttccca tgcttgattt ttccagtctt
      301 tctcgtgatg gtcataccac attgattcat cctggttatg ttgtatcggc taaacatggt
      361 ggtttacaaa gtgtttcatc agcaactttt ggttatgacc agatatataa aatagttgat
      421 aataaccttg ctggtataga tttttctgcc ccacgattaa ataagcttg
//
LOCUS       DAZUGK010000618          467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZUGK010000618 DAZUGK010000000
VERSION     DAZUGK010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.624"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>467
                     /locus_tag="ACHY5W_005226"
     CDS             <1..>467
                     /locus_tag="ACHY5W_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4449895.1"
                     /translation="LLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQH
                     DDWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKV
                     RTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWRGFVYVAFIIDVFAG"
     misc_feature    2..66
                     /locus_tag="ACHY5W_005226"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          107 a          111 c          145 g          104 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagcga actgcatatt
       61 gccccgtcaa cgtattacca ttgtcagcaa cagcgacatc atccggataa acgcagtgcc
      121 cgtgcgcagc acgatgactg gctgaagaaa gagatacagc gcgtatacga tgaaaatcac
      181 caggtatacg gtgtgcgtaa agtctggcgt cagttgttac gggaaggtat cagagtggcc
      241 agatgtacag tggcgcgcct catggcagtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgca ctaccgtcag ccggaaagcc gttgccgcat gcgaccgcgt aaaccgtcag
      361 ttcgtggcag aacgtccaga ccagttgtgg gtggctgatt ttacctgggt aagcacatgg
      421 cggggcttcg tctatgtggc gttcatcatt gatgtgtttg ccggata
//
LOCUS       DAZUGK010000619          463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZUGK010000619 DAZUGK010000000
VERSION     DAZUGK010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.625"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..213)
                     /locus_tag="ACHY5W_005227"
     CDS             complement(<1..213)
                     /locus_tag="ACHY5W_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4449896.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQF"
     gene            255..>463
                     /locus_tag="ACHY5W_005228"
     CDS             255..>463
                     /locus_tag="ACHY5W_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS4449897.1"
                     /translation="MSPKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYAL"
BASE COUNT          142 a          126 c          102 g           93 t
ORIGIN      
        1 gaactgaagt gcaattcggt gatcgagttc actgtcacag aggtcaacca cacgaccaca
       61 gagacagcac tgcccggcat ccctgagcca gatacggcgc ttgagggaaa cacgtgcact
      121 gccactgacc cgacgctgtt cacccttcag gacattcacc cgccgggtgt tcagagtttt
      181 gattctgccc ggtaacgtac gaagcacagc catgtaaaat cctcgccata taacttgtca
      241 ccagaggaaa gaaaatgtca ccgaaaaacc ggacccgcag aacaacaacc cgcaacatcc
      301 gatttccaaa ccagatgatt gaacaaatta acatcgctct tgaccagaaa ggttcagaaa
      361 atttttctgc gtgggtcatt gaatcttgcc gccgggagct ggcagcagac ataaaatatg
      421 cccgtcagtt gactataaaa aagaatgata cacagtatgc tct
//
LOCUS       DAZUGK010000620          461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZUGK010000620 DAZUGK010000000
VERSION     DAZUGK010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.626"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..430
                     /locus_tag="ACHY5W_005229"
     CDS             <1..430
                     /locus_tag="ACHY5W_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449898.1"
                     /translation="PELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILSR
                     VYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISEM
                     LIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT          133 a           88 c           85 g          155 t
ORIGIN      
        1 tcctgaactt tcagcacttc caccttcatt aagaaagaaa aattttactg acgcatataa
       61 tgcagcatct tctcatatcg aatactggat aggtgctggc gtctcattta tctcgatgat
      121 gattttatcc agagtatatg actttctttt acccgcccaa gatactttcc cgggtgatat
      181 catcagaagt ttatgtgttg tgtgtccatc catattaatc tggtttcagt tttcggtata
      241 tgtcatgagg aaatattaca gacatattct ggtgcgagga aaagaaaccg aaaccatttc
      301 agaaatgttg atacgtgagg ctgatacacg agaatatgaa ctatggcgtc cggtccgtcg
      361 ctttttctcc attgttttcc tcattgttct attaggttgc attcattcac tgataacaac
      421 tataaaataa aagttatggc tgttctgata caggaaaata a
//
LOCUS       DAZUGK010000621          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZUGK010000621 DAZUGK010000000
VERSION     DAZUGK010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.627"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..124)
                     /locus_tag="ACHY5W_005230"
     CDS             complement(<1..124)
                     /locus_tag="ACHY5W_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449899.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIV"
     gene            complement(380..>452)
                     /locus_tag="ACHY5W_005231"
     CDS             complement(380..>452)
                     /locus_tag="ACHY5W_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFS4449900.1"
                     /translation="EQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          132 a           93 c           87 g          140 t
ORIGIN      
        1 agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttcta ttgtgattgt
       61 taaccactgc atgcattggt gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt
      121 tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattt tcctcgcagg
      181 gttctttcgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac gaagcaacat
      241 tctgtggcct acgccgatca aaagctctgt gaaaatttcc cttgttgtct ttgtctgacc
      301 ttatcgcggc gaccagcaat tattcatttt gtgatcctca acacaaaatg ctattcgtcg
      361 cctatgaggg gagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcag
      421 aagagaaaaa gttgacgtta atcaattgtt cg
//
LOCUS       DAZUGK010000622          449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZUGK010000622 DAZUGK010000000
VERSION     DAZUGK010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.628"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>449
                     /locus_tag="ACHY5W_005232"
     CDS             <1..>449
                     /locus_tag="ACHY5W_005232"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4449901.1"
                     /translation="VFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPP"
BASE COUNT          116 a          101 c          126 g          106 t
ORIGIN      
        1 atgtctttga aggcatcgcc atttttaatg ccctgaaaaa tcagggggcg accattaccg
       61 tgtatgtgga tggcgttgcc gcctcgatgg catctgtgat tgcgatggcc ggtgatacgg
      121 tcattatgcc ggaaaatgcc ttcatgatga tccataagcc atggggattc agtggcgggg
      181 atgctgagga tatgcgcagt tatgccgatt tgctggataa agtcgaatcg gtactgttgc
      241 cagcctatgc gcagaaaacc ggaaaaacca ccgatgaaat tgccgccatg ctggcggatg
      301 aaacctggat gtccggtgcc gaatgtctgg cacacggatt tgctgaccag gtgacacccg
      361 ctgttgaggc aatggcatgt attcagtcaa aacgtacaga ggaatttaaa aagatgccgg
      421 aatccatccg aaacatgatt actccgcca
//
LOCUS       DAZUGK010000623          448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZUGK010000623 DAZUGK010000000
VERSION     DAZUGK010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.629"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>448
                     /locus_tag="ACHY5W_005233"
     CDS             <1..>448
                     /locus_tag="ACHY5W_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4449902.1"
                     /translation="LLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQS
                     LEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIY
                     ALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYF"
BASE COUNT          138 a           80 c           80 g          150 t
ORIGIN      
        1 ttgcttatga gaatgaatga cttaaataac gatttagtta attattcgca tgataacacc
       61 agaacttcag tcggtgcccg tctggcaatg tatgaagttg gcttaaaaac atattctcca
      121 ataggacaat cactggaaaa acgtgcagaa aaaatacatg aactagaaga aaaagagcct
      181 agattgagtg gcgctttacc ctatgtagat tctcatttgc ataacgatct catagatacg
      241 ttatcaacgc gtggtattcc tggagttgta ttaacaattt tagcattttc agcaatactc
      301 atatatgcct taagaactgc taaagaacct tatattttga tcttgctttt ttcactactg
      361 gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc tgtgtttgtc
      421 accataatat tgctttgtgc ttatttta
//
LOCUS       DAZUGK010000624          445 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZUGK010000624 DAZUGK010000000
VERSION     DAZUGK010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.630"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(123..197)
                     /locus_tag="ACHY5W_005234"
                     /pseudo
     CDS             complement(123..197)
                     /locus_tag="ACHY5W_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(194..>445)
                     /locus_tag="ACHY5W_005235"
     CDS             complement(194..>445)
                     /locus_tag="ACHY5W_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4449903.1"
                     /translation="PGRFNLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
BASE COUNT          110 a          109 c          106 g          120 t
ORIGIN      
        1 aaacatataa taattttcat caaaatagta tttatgtact aactaagctg gatttgaacc
       61 tctatttctg ggtatttgtt atcacttaat ccattactgc tcctccttca gcatatgtcg
      121 tatccgttga aggatttacg catatcggta acgtcggcaa cgagccagat gcgggtacct
      181 gacgggagtg agatcatcgt cccctcccgg tcagttcacg gatcaacagc gtgagcagct
      241 ctggcgatgg attttccagc gtcatgttac cgtgacggaa ctccaccttg caggagccgg
      301 cactgacact ggtctgagtg gaagtggata aagacggcgc aatggccgcc accggttctt
      361 tctgctcatc cggcgttatt tctacaggta ataattcaac gccagtgtca gaagaggtcg
      421 ttacctgaag attgaaccgc cccgg
//
LOCUS       DAZUGK010000625          443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZUGK010000625 DAZUGK010000000
VERSION     DAZUGK010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.631"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..77
                     /locus_tag="ACHY5W_005236"
                     /pseudo
     CDS             1..77
                     /locus_tag="ACHY5W_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(272..385)
                     /locus_tag="ACHY5W_005237"
     CDS             complement(272..385)
                     /locus_tag="ACHY5W_005237"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFS4449904.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          136 a          120 c           97 g           90 t
ORIGIN      
        1 cggaatcaaa tcacaggatg accatctgcc agtggctggt cgtaaaaaaa aggccgcgcc
       61 atgcgcagcc gaaaataaag ggataacgat gatagtttga gaaaaacaga aataacactt
      121 ttgtggcaaa gcatggtgcc gggtgcctcc cggtgaattc agtatcagca cctgaatccg
      181 cgattacccc atattccttc ttgctgattg ccccaccgca cagggggatt caccatgcag
      241 aagtgttttt aataaacagc aaacaaaaaa atcaagcatt atgcaggctg tttcttttta
      301 tcaccggcca cagcaatacc ataatgccgc agaccagcac cccatccgcc agcaccgaca
      361 tgattctgct ggtgaaatcc accatcacca ccagaaacag caggagtgca gccacagtca
      421 ggcgcagttt taccgtcaca ggt
//
LOCUS       DAZUGK010000626          437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZUGK010000626 DAZUGK010000000
VERSION     DAZUGK010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.632"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..107)
                     /locus_tag="ACHY5W_005238"
     CDS             complement(<1..107)
                     /locus_tag="ACHY5W_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449905.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGT"
BASE COUNT          127 a          100 c           93 g          117 t
ORIGIN      
        1 tctgtaccat aagcctcctc agcgaggttg cctgaatacc ctgtcgtgcc catttttcca
       61 tcggacgcag ttgtctgctc tctcgaactg ctggcaataa aaatcaacca gacagcttga
      121 tgtccgttac atgcaggact aatcacgatt ggttaccgtt tacaaaactt gcgtaacatt
      181 ggaggagaac gaataatatt ctctagctgg tacgcgttcc atagcgaaac gggcaaggtc
      241 caacagcaag agctgaaact cagggggatt tatcagtcga tttattcgcc atggatgaat
      301 cacgctgtac ggttatggta gctcttagcg acaaggcata atcatcagta caccaatgta
      361 acaaccgttg aaaaagactc attattttat gaaccagcca tcccatttcc agagtggtaa
      421 atatgatttg ttaaagt
//
LOCUS       DAZUGK010000627          430 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZUGK010000627 DAZUGK010000000
VERSION     DAZUGK010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.633"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>430)
                     /locus_tag="ACHY5W_005239"
     CDS             complement(<1..>430)
                     /locus_tag="ACHY5W_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449906.1"
                     /translation="ARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQ
                     PSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNK
                     KLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLY"
BASE COUNT           97 a          116 c           94 g          123 t
ORIGIN      
        1 atataaaggc gtccgtcctt catctgttgc agcttcacag cccacatgct gttcagatta
       61 ttatttgtat caacctgaac cttctgtatc tgctggatcg ctgcactctg gtcttccagt
      121 ttcttattga cggtctgcgt gatttcatta ctgacatccg tgatggacgt tctgatttcc
      181 gccaggtcag gcgcaagctg accgttatca atctgagtcc acaactcctg agccagatgg
      241 gttttcccta tctctccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg
      301 ccaacagcct ccacaaatgc cgatttgcca acggtgttca cactgcggat ataaaagtaa
      361 taatcatggc ccggtttgat attgatactg gcggctatcc agtacagccc cgtgccaagg
      421 tagcgggctg
//
LOCUS       DAZUGK010000628          429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZUGK010000628 DAZUGK010000000
VERSION     DAZUGK010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.634"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>429
                     /locus_tag="ACHY5W_005240"
     CDS             <1..>429
                     /locus_tag="ACHY5W_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449907.1"
                     /translation="LMSAPEFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDIL
                     LLYRFFKMDGDFNILTSDEYMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQI
                     YFHIFPDESATLVLYFSNNDIECFVFERDIPSQYRFFKMFT"
BASE COUNT          129 a           90 c           72 g          138 t
ORIGIN      
        1 attgatgagc gctccggaat ttatgttcaa attcagttat agcgctcaac aaaatagatt
       61 agaacctcat cttctggttc ccgcttattt ccctctaaaa acaaacgaac cggttacgca
      121 agaggatata ctgttacttt atcgtttctt taaaatggat ggtgatttta atatattaac
      181 atcagatgaa tacatgagta tcatgacacc tctgatgaag tgtgagcgca gtatccacga
      241 ccacaacagg tatgtgacgg gcaaagatac ccttcttctg gactatcctc cttctggtaa
      301 ccagatatat ttccatattt tccctgacga atcagcaaca ctagtccttt atttttcaaa
      361 caatgacatt gaatgtttcg tctttgaaag agatattccg agccagtatc gctttttcaa
      421 aatgtttac
//
LOCUS       DAZUGK010000629          422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZUGK010000629 DAZUGK010000000
VERSION     DAZUGK010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.635"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>422
                     /locus_tag="ACHY5W_005241"
     CDS             <1..>422
                     /locus_tag="ACHY5W_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS4449908.1"
                     /translation="FFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALN
                     GYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLD
                     ILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNT"
BASE COUNT          137 a           72 c           90 g          123 t
ORIGIN      
        1 ttttttgttt ctcctggcga gtttcgtcag actggaacgg atgcatcccg cacaataaat
       61 gaaaaacaaa agccggtaat tatgttttta gttgtcgggg agacagcaag aagtcaaaac
      121 tatgctctga atggatattc tcgtggtacc aatgatttta caaagaaata taatgaactt
      181 atatcctttc ataatgtgca gtcgtgtggc acatccacag ccatatctgt tccatgcatg
      241 ttttcagata tgaagcgaaa agagttcaat tcccgcaaag cggtaaattc ggaaaatgta
      301 ctggatattc tgtacagaac gggggtaaat ttactctgga ttgaaaatga cgggggatgt
      361 aaaggtgtat gtaagcgaat ccctacaatt aatattgagc catcaaattc agataataca
      421 tt
//
LOCUS       DAZUGK010000630          417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZUGK010000630 DAZUGK010000000
VERSION     DAZUGK010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.636"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>417
                     /locus_tag="ACHY5W_005242"
     CDS             <1..>417
                     /locus_tag="ACHY5W_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449909.1"
                     /translation="HIKQLEEWALLEKFYDNNAYYSKRTKQLEKKITDAGFITGKVNA
                     EKFNVKEFKDDITSMLCEQPPLRTHQISSSGYVSADKLPKLYELNLKDAQRDLKTTPV
                     DIDGVSLKFKTRERIAEEIAKKTAGKTAEKNSEIIAK"
BASE COUNT          158 a           71 c           88 g          100 t
ORIGIN      
        1 cacataaagc aacttgagga gtgggctctt cttgaaaagt tttatgataa caacgcttat
       61 tattctaaac gtacaaaaca attagagaag aaaattaccg atgcggggtt tataacaggg
      121 aaggttaatg ccgagaagtt taatgtaaaa gagtttaaag acgatataac ctcgatgctt
      181 tgtgaacagc cgcccctgcg tacacaccag atttcttcat ccggatacgt atctgcagat
      241 aaactgccca aactctatga gttaaatttg aaagacgcgc agagagatct taaaacaaca
      301 ccggttgaca tcgatggtgt cagtttaaaa tttaaaactc gggaaagaat tgcagaagaa
      361 attgcgaaaa aaactgcggg aaaaactgcg gaaaaaaatt cggaaataat tgcgaaa
//
LOCUS       DAZUGK010000631          416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZUGK010000631 DAZUGK010000000
VERSION     DAZUGK010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.637"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          116 a          109 c           94 g           97 t
ORIGIN      
        1 ttgtgactgg cttaaaaaat cattaattaa taataggtta tgtttagaga gttccccgcg
       61 ccagcgggga taaaccgcgc ctggctgtaa taatgcacgg cagtgtgacg agttccccgc
      121 gccagcgggg ataaaccgca aaatcctgtc ggaaaaaatc gacaatcatg agttccccgc
      181 gccagcgggg ataaaccgcg atcttttcct acctggtaca atcagtaaat gtgttccccg
      241 cggcagcggg gataaaccgg gtacaaaaga ggccccattc tggcagggct caagttcccc
      301 gcgccagtgg ggataaacca ccatataacc cgttatctct ttctcaagtt tttatattag
      361 cagtactcgt aataagcaac atatccacgt aacccctcat gttcaaaata gttccc
//
LOCUS       DAZUGK010000632          409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZUGK010000632 DAZUGK010000000
VERSION     DAZUGK010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.638"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..312
                     /locus_tag="ACHY5W_005243"
     CDS             37..312
                     /locus_tag="ACHY5W_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFS4449910.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
BASE COUNT          165 a          101 c           66 g           77 t
ORIGIN      
        1 cataaaaaca cttttcatgg agcaaaggag aaaacaatgc cattttcaat caaaaacaga
       61 ttttcaagtt cacaagtaca ttacccggaa atatccggtc ccataaaaga caagccagcg
      121 tcaaagaact gcatacttac atcaacaaca tgtaatgtag atagctatac agtgtaccaa
      181 aaaaaagcct gtagttttga catgcgccca cccggcgcag gagaaagaac cccaaaacta
      241 aaactctcag ttactgagat gacatggcta tctaaaacta tagaaacaga gatacacaac
      301 acaaaagaat agcaaccact taccagagaa cacaaaaagc cacagacccg aaacacctga
      361 ctgcaagcaa ccacctccac aggaggtggt tttaccatga acttcctgt
//
LOCUS       DAZUGK010000633          409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZUGK010000633 DAZUGK010000000
VERSION     DAZUGK010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.639"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>409
                     /locus_tag="ACHY5W_005244"
     CDS             <1..>409
                     /locus_tag="ACHY5W_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS4449911.1"
                     /translation="DGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKY
                     LTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSA
                     NQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRV"
BASE COUNT          113 a           96 c          115 g           85 t
ORIGIN      
        1 gacggcatgc agagtcaggt gctgctggca gcagacagga ttgcgatgat taatcctgcg
       61 aatggcaaca caaagccgat gtttgttggt cagggcgatc agatattcat gaatgaagtg
      121 ttcctgaaat atctgacggc tcccaccatt accagcggcg gtaatcctcc ggcattttcc
      181 ctgacaccag acggaaagct gaccgctaaa aatgcagata tcagtggcag tgtgaatgcg
      241 aacgccggga cgctcaacaa tgtcacaatt aatgagaact gtcagattaa ggggaaactg
      301 tcagccaacc agattgaagg cgatatagtc aaaacagtgg gtaaggcttt tccgcgggac
      361 tcccgggcac cggagcggtg gccatcaggg accattaccg tcagggttt
//
LOCUS       DAZUGK010000634          406 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZUGK010000634 DAZUGK010000000
VERSION     DAZUGK010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.640"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..393
                     /locus_tag="ACHY5W_005245"
     CDS             <1..393
                     /locus_tag="ACHY5W_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HFS4449912.1"
                     /translation="DDTLKLFKRDLIEQESRKAAGEIDQDLVEKRPHLARKKMREILT
                     AKIKEEASNLLIINGLDEKGQELQPIFSPHLRNLQTTDANDAIIVKFILGKLYRQFGP
                     VKDRDNQTLQNSINSIPEILAEVSRMLK"
BASE COUNT          154 a           65 c           78 g          109 t
ORIGIN      
        1 gatgacactc tgaaattgtt taaaagagac ttaattgaac aagaatctag gaaagctgct
       61 ggagaaatag atcaagatct agtagaaaaa aggccgcatc ttgctcgtaa aaaaatgaga
      121 gagatcttaa ccgctaagat taaagaagag gcttctaacc ttctgataat aaatggcctc
      181 gatgagaaag gacaagaact gcaacctatt ttttcaccac atcttaggaa tctacagaca
      241 accgatgcaa atgatgctat tattgtaaaa ttcattttgg gtaaattgta tagacagttt
      301 ggtcctgtaa aagatcgaga caaccaaact ttacagaact caattaattc aatacctgag
      361 atcttagctg aagtgtcgag gatgttaaaa tgattaatgt agctca
//
LOCUS       DAZUGK010000635          403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZUGK010000635 DAZUGK010000000
VERSION     DAZUGK010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.641"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..374
                     /locus_tag="ACHY5W_005246"
     CDS             <1..374
                     /locus_tag="ACHY5W_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS4449913.1"
                     /translation="ADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVN
                     DISGLAFNNESLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTL
                     PDESIYVASEPDILAKFVPNK"
BASE COUNT          124 a           84 c           78 g          117 t
ORIGIN      
        1 aagcagatga tcggatgctg tttgtcgcaa aagaaagacg cccaagtaaa atatttatat
       61 atcaactttc tccagacctg ctgagtgtaa agcaagcaac tattccagag gcactaaatg
      121 atatacgtgt taacgatata tccggcctgg cctttaataa tgaaagtctg atgattctgt
      181 ccgatgaatc gcgtaaatta ctcaagttta acctgacaga gatgtctttc attgagatgc
      241 ttgacctgac aaaaggaaat cattctctga ccagtgattt gccccaacct gaaggaattg
      301 taactctgcc tgatgaaagc atttatgttg ccagtgaacc tgatatcctc gcgaaatttg
      361 tccctaataa gtaagtctgc cactggcctt attatttatc gac
//
LOCUS       DAZUGK010000636          399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZUGK010000636 DAZUGK010000000
VERSION     DAZUGK010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.642"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..79)
                     /locus_tag="ACHY5W_005247"
     CDS             complement(<1..79)
                     /locus_tag="ACHY5W_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449914.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKA"
     gene            97..288
                     /gene="yfgG"
                     /locus_tag="ACHY5W_005248"
     CDS             97..288
                     /gene="yfgG"
                     /locus_tag="ACHY5W_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFS4449915.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT           98 a          104 c           77 g          120 t
ORIGIN      
        1 acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac
       61 cgtccacggc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga
      121 aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc
      181 ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa
      241 gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct
      301 ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc
      361 aaagcggcag atattctttc atcttaaatt ttacgtctt
//
LOCUS       DAZUGK010000637          393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZUGK010000637 DAZUGK010000000
VERSION     DAZUGK010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.643"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>393)
                     /locus_tag="ACHY5W_005249"
     CDS             complement(<1..>393)
                     /locus_tag="ACHY5W_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFS4449916.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKLA
                     LSGINIPIKDIILLGSVDIRRFIKKLIES"
BASE COUNT          121 a           89 c           70 g          113 t
ORIGIN      
        1 gattcaataa gttttttgat aaaacgacga atatccacag atcccagtaa aataatgtct
       61 ttaataggaa tattgattcc agaaagtgcc agtttaaaat tatccagaat catctcagct
      121 tcggatgcat cgagattaag aaaagtaccg ccagcggtag gcctgacacc atctctgatt
      181 ctgtcctcta tttcaggcga aatcacaatt gctttaattt ctccagaata tgaaaactta
      241 tggcaaatgt agcgacctaa tgcacctcgt acatgctcga cgagtgtgat tatatctttc
      301 tctcgtgggc tccataatgc taaggcctcc ataactaacc gcatattgcg cactgaaatt
      361 cgctcctgga tcagacgctg aattacctct gaa
//
LOCUS       DAZUGK010000638          392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZUGK010000638 DAZUGK010000000
VERSION     DAZUGK010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.644"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          131 a           78 c           74 g          109 t
ORIGIN      
        1 aagtagtgat gtatcacgta aaaatatagt catcatagat gttatgcagt acgcacaaaa
       61 aaacagactg gatttgcttg ctgatctata atctttatct aaattactat tggcgctttg
      121 gataataaca taagcatata ttacctccta gcaataatag acataacctc atatcaaggt
      181 gtcactggta aataatcgga taaaagacag tatcggtcgg cgcatagagc ttcttctatt
      241 tcaccagaag tgcacagcag ggaactacca atcatacggt tagtgtcatg ggtggaggat
      301 gcgacccgtt ctttccctat aacggggata cgcagtttct atcagtacat gcagaaaacc
      361 cctaattcac atcactgcag aaagcattta at
//
LOCUS       DAZUGK010000639          381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZUGK010000639 DAZUGK010000000
VERSION     DAZUGK010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.645"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          137 a           84 c           53 g          107 t
ORIGIN      
        1 ataaatattg aatacattta ctgagcatgc aaaagaaaaa gacaaactaa tcccggcctt
       61 tcacagaatt tcctctatca gaggctgagt tgacaacctg ctcacccagg aaatagtaac
      121 acccgaaaac atgaagagac tctgataaac aatctcgata catattcctc atctttcaca
      181 gcatctcact tctttaacag gaattattat tatctacgcc tcctcctttc atgtatccct
      241 catgaatgat taagataaac atactgtgtt tgttatccgt atgataacaa acacataaag
      301 gagtaaacat ttatctggat gatgattaca atgttcacga ctgtcagaga cacacctctg
      361 atacattaga aaaaccaatg t
//
LOCUS       DAZUGK010000640          379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZUGK010000640 DAZUGK010000000
VERSION     DAZUGK010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.646"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..>379
                     /locus_tag="ACHY5W_005250"
     CDS             119..>379
                     /locus_tag="ACHY5W_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFS4449917.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDQIRFDATLDKNEAYRDADYVIIATPTDYDP"
BASE COUNT          113 a           76 c           72 g          118 t
ORIGIN      
        1 aatctgaatt gtttttcgtt gcgttgacca gctatcgacg tagcgttaaa acttttaact
       61 cttatcagga tgctaaaaac atcatgattc acagttaagt taattctgag agcatgaaat
      121 gaaaatcacc atttccggta ctggctatgt aggcttgtca aacgggcttc taatcgcaca
      181 aaatcatgag gttgtggcat tagatatttt accgtcacgc gttgctatgc taaatgatcg
      241 gatatctcct attgttgata aggaaattca gcagtttttg caatcagatc aaatacgctt
      301 tgatgccaca ctagataaaa atgaagccta tcgggatgct gattatgtca tcatcgccac
      361 tccaaccgac tatgatcct
//
LOCUS       DAZUGK010000641          376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZUGK010000641 DAZUGK010000000
VERSION     DAZUGK010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.647"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /locus_tag="ACHY5W_005251"
     CDS             <1..165
                     /locus_tag="ACHY5W_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFS4449918.1"
                     /translation="PIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAP
                     HLYFKRKNWL"
     gene            complement(190..>376)
                     /locus_tag="ACHY5W_005252"
     CDS             complement(190..>376)
                     /locus_tag="ACHY5W_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449919.1"
                     /translation="GCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFT
                     TIMLSAIPSAKMSLSAH"
BASE COUNT          106 a           81 c           84 g          105 t
ORIGIN      
        1 ccgattagga aactttttcc tacgcggatt gcctccagct atgggatgaa ctttgagttt
       61 atgccagaac tgaagtggag tttcggctac cctggcgcga ttatctttat gatcctcgcg
      121 ggcctggcac cgcatctgta ctttaagcgt aagaactggc tgtaactata cgatgtcgct
      181 gtgagtaagt tagtgtgctg acagcgacat tttagccgag ggaatcgcag ataacataat
      241 tgttgtgaat aatatccatg ccacgttgag ttggacgctt aatgactcac atctcacccc
      301 ttcaattgct gcagagataa tagaaaaaag tgttaaaccc aatgaaatca atacaaagta
      361 gattgcctta catcca
//
LOCUS       DAZUGK010000642          372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZUGK010000642 DAZUGK010000000
VERSION     DAZUGK010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.648"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>372
                     /locus_tag="ACHY5W_005253"
     CDS             <1..>372
                     /locus_tag="ACHY5W_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449920.1"
                     /translation="PRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDTGN
                     LAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQ
                     TGLNFSPITVSHNIHAGTQLNP"
BASE COUNT          115 a           80 c           67 g          110 t
ORIGIN      
        1 ccgcgtcaat ataacttctt cttaaccaga aaagaatctg tcattcttaa tgaagtactc
       61 aatcgatttg ttgatgcttt aacaaatgaa gttcgctatg aagtatcaca aaattggctt
      121 gatacaggaa acctggcctt tctgaacaag ccattagaac tcactgaaca tgaaaaacag
      181 tggattaagc agcatcccga tttaaaggtg ctggaaaatc cttactcccc tccctattct
      241 atgacggatg aaaatggctc ggttcggggc gttatggggg atattcttaa tattattact
      301 ttgcaaacag gtttaaattt ttctccgatc accgtttcac acaatatcca tgctggaaca
      361 cagctgaatc cc
//
LOCUS       DAZUGK010000643          369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZUGK010000643 DAZUGK010000000
VERSION     DAZUGK010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.649"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(81..317)
                     /gene="ycgZ"
                     /locus_tag="ACHY5W_005254"
     CDS             complement(81..317)
                     /gene="ycgZ"
                     /locus_tag="ACHY5W_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFS4449921.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          107 a           79 c           62 g          121 t
ORIGIN      
        1 atttcatatt ttatactttc attatcagat gtcttcataa cgacttcata gctaacctgt
       61 taaataatca ggctgttgca ttattcaaaa agcaacccaa ttagtgcatt ataatgtttc
      121 tgttcctctt cccctgtcgc ttgttccagt cggcaaagaa gtttggcaca aagcgacttt
      181 cgactcagat tacgtccatc tttcaaaatc tcagtcacaa tttccccgag agtttcctgc
      241 tgagtaggca agttagcttt tgcaaagtaa cgagtgattg ctcccgcaga atctaaagtc
      301 actgaatttt gatgcatgct acgcctctgt taaaaatgtt aatcaatgtt aagtgaggta
      361 acaatagca
//
LOCUS       DAZUGK010000644          368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZUGK010000644 DAZUGK010000000
VERSION     DAZUGK010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.650"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>368
                     /locus_tag="ACHY5W_005255"
     CDS             <1..>368
                     /locus_tag="ACHY5W_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFS4449922.1"
                     /translation="YLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSV
                     ASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQ
                     ISANDEVLFLKQGHEIIAKN"
BASE COUNT          107 a           72 c           84 g          105 t
ORIGIN      
        1 tatctctttt cgcggagggc gaacgataaa atcgttgttt atcatcagga acaagtaaat
       61 ggaccgcttg tcgacgagtc agggcgggtt atttttgccg attttaaccc atcgaaacga
      121 ccgtggtcgg tggcttcaga tgactctaac aacagctgga atccggcata caattgcttt
      181 gatcgtccgg gtaaaaaatg tatctctttt acgctacgca tcaacggcaa agatcacgat
      241 ttgttagcgg tggataaaat tcatgtcgat ttaaactggc gatatctgaa cgagtatctt
      301 gatcaaatca gcgctaatga tgaagttcta tttttgaaac aaggccatga gattattgcc
      361 aagaatca
//
LOCUS       DAZUGK010000645          366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZUGK010000645 DAZUGK010000000
VERSION     DAZUGK010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.denovo.651"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>366)
                     /locus_tag="ACHY5W_005256"
     CDS             complement(<1..>366)
                     /locus_tag="ACHY5W_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4449923.1"
                     /translation="HLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARL
                     VTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPG
                     YFQITATPHLAVYDPTVQF"
BASE COUNT           62 a          118 c          112 g           74 t
ORIGIN      
        1 tcaaactgta ccgtcgggtc ataaacggca agatgcggcg tggcggttat ctgaaaatag
       61 cccggcgtca gctcaatccg cgacggtgct gccggtgcgg caatccggaa ggtggtggtg
      121 gcaggttcac cctgctggcc atagctgttt atcgcccgca ccgtcagggt gtattccccg
      181 agcggcaggc cgctgaaacg gtgctccgtg tctgcggtga tggcggtggt caccagtctg
      241 gcatccgttc ccttaccact ggtcaggcgc agactgaagc gcacgccctt caccacccgc
      301 ggcgtgtccc atttagcctg cgccagatac tggccgtcag ctgcactcac ctccaccgtc
      361 aggtgc
//
LOCUS       DAZUGK010000646        15278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.guided.105, whole genome shotgun sequence.
ACCESSION   DAZUGK010000646 DAZUGK010000000
VERSION     DAZUGK010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.guided.105"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHY5W_005257"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHY5W_005257"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFS4449924.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPAILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHY5W_005258"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHY5W_005258"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFS4449925.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKLAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHY5W_005259"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHY5W_005259"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFS4449926.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHY5W_005260"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHY5W_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFS4449927.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHY5W_005261"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHY5W_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFS4449928.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHY5W_005262"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHY5W_005262"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS4449929.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHY5W_005263"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHY5W_005263"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS4449930.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHY5W_005264"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHY5W_005264"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS4449931.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHY5W_005265"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHY5W_005265"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS4449932.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHY5W_005266"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHY5W_005266"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS4449933.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /gene="ampC"
                     /locus_tag="ACHY5W_005267"
                     /pseudo
     CDS             12901..14034
                     /gene="ampC"
                     /locus_tag="ACHY5W_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="ACHY5W_005268"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="ACHY5W_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS4449934.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYHHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHY5W_005269"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHY5W_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS4449935.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKALPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15151..>15278)
                     /gene="ecnB"
                     /locus_tag="ACHY5W_005270"
     CDS             complement(15151..>15278)
                     /gene="ecnB"
                     /locus_tag="ACHY5W_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS4449936.1"
                     /translation="AIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAATKAQQ"
BASE COUNT         3779 a         4047 c         3969 g         3483 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgtatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 agcaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtcg agaattgccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgatttaccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc acgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaactggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaat aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 gtctcggtca acacaccacg accgccgcac gcctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct tgaagcccta ctggcaggca
     2401 actatctgat ggctgacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ctgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga ctgtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccaggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaacga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgactgccga gcaaaaccgt attctcgaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacactac tgctggaact gaccaagctg aaagtctcta
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gccgccttat ctcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc tcgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataatctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccaacgacgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcag tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcaactg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggtt
     5641 acgccatcac caccatcacc aaatacctgt tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacga
     5881 aaattaatac ccgcgccact acaatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata ggatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aaagccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgcca aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acccgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat ccccttagaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataacac
     8041 gtgcccactg aactgcactg cgcaacattg agcaggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttaacgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acgcctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttgcc aatatttggc tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagt gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cagcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccgaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtggggat gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgta ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgcca cgtggtgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggtcg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggctgcgg
    10921 tatttaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agcttcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccatg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cttgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtagttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgccatca ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 ccccaaaaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atcccgggta tggcggtggc ggtaatttat cagggtaaac cttattactt tatctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc
    13261 acactattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacataac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacacctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcaccaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacacgcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctgca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgccatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgctt actccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgct aaacgctatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg agggatgatg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccac catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc agacgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgcttt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcaga ggaatttcaa ctattacctt tgtgactatc gcaaaaaaac aaacggcacg
    15121 acacagctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga
    15181 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
    15241 taatactgtt gaaagcacca gaacagaaaa gatcgctg
//
LOCUS       DAZUGK010000647         6485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.guided.229, whole genome shotgun sequence.
ACCESSION   DAZUGK010000647 DAZUGK010000000
VERSION     DAZUGK010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.guided.229"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..655
                     /locus_tag="ACHY5W_005271"
                     /pseudo
     CDS             26..655
                     /locus_tag="ACHY5W_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            652..1266
                     /gene="prfH"
                     /locus_tag="ACHY5W_005272"
     CDS             652..1266
                     /gene="prfH"
                     /locus_tag="ACHY5W_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFS4449937.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVVVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVRKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1323..2780)
                     /gene="pepD"
                     /locus_tag="ACHY5W_005273"
     CDS             complement(1323..2780)
                     /gene="pepD"
                     /locus_tag="ACHY5W_005273"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS4449938.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVAHYWTLLTELLKEIPAK"
     gene            3041..3499
                     /gene="gpt"
                     /locus_tag="ACHY5W_005274"
     CDS             3041..3499
                     /gene="gpt"
                     /locus_tag="ACHY5W_005274"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4449939.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3591..4835
                     /gene="frsA"
                     /locus_tag="ACHY5W_005275"
     CDS             3591..4835
                     /gene="frsA"
                     /locus_tag="ACHY5W_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS4449940.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDNYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSMLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKALQEITNWIEKRLC"
     gene            4893..5294
                     /gene="crl"
                     /locus_tag="ACHY5W_005276"
     CDS             4893..5294
                     /gene="crl"
                     /locus_tag="ACHY5W_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS4449941.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5333..6388)
                     /gene="phoE"
                     /locus_tag="ACHY5W_005277"
     CDS             complement(5333..6388)
                     /gene="phoE"
                     /locus_tag="ACHY5W_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS4449942.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDIYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     MQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLNSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1594 a         1579 c         1663 g         1649 t
ORIGIN      
        1 ctaacacccc attataaggg gtacgaataa agtaacgggg cagtctattt tacagcggca
       61 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgatgcgc tacggtacat
      121 tgcggaacat gacgatgcgc tggcatttgc gcgtattaat agccagatga tcgctttgcg
      181 cataatgcaa caggttaagg ccacgggaag tccggttctg gatgtggcgc ataactttgt
      241 tagcgcgtgt caaatcggcg atcaacaggg ctggttgcat cgtaaaggtg ccacgccgga
      301 tgacaacggt ctggtgatta ttcccggttc acgcggtgat tactcctggc ttgtacagcc
      361 tgttgcgaac gaaaaaacgc tccactcgct ggcgcatggg gcagggcgta aatgggggcg
      421 caccgagtgt aaagggcgtc tggcagcgaa atacacggcg acgcaactct cgcgtactga
      481 acttggcagt cgggtaattt gtcgcgataa acaactcatc tttgaagaag cgccacaagc
      541 ctataaatcg gctgaaagcg tggtgcaatg tctggtgcag gccggattaa ttattcctgt
      601 cgtgcggctg cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag gatgatcttg
      661 ctacaactct cctctgctca ggggccggaa gaatgttgtc tcgcagtgaa aaaggcgctg
      721 gacaggctga ttaaagaagc tgctcgacaa gacgtcgtgg taacagtgct ggaaacagaa
      781 acaggtcgtt actctgacac gctgcgttcg gcgctgattt ctctggatgg cgacaacgca
      841 tgggcgttaa gcgaaagttg gtgcggcact attcagtgga tttgtccgag tccgtatcgg
      901 cctcatcatg ggcgcaaaaa ctggtttctg ggcattgggc gttttaccgc tgatgagcag
      961 gagcaatccg atgcgatccg gtatgagacg ctgcgttcgt cggggccggg tggtcaacat
     1021 gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat tagcgtgaag
     1081 gttcagtcag aacgcagtca gcatgctaac aagcggctgg cacgattgct gattgcctgg
     1141 aagctggagc aacagcaaca ggaaaatagc gcagtgcgga aatcgcagcg gcgaatgttc
     1201 catcaccaga ttgaacgtgg caacccgcga cgaacgttca cagggatggc ttttatcgaa
     1261 ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag caaatcaaat
     1321 aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta atgagctacg
     1381 ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgattgttgg cccgatagaa
     1441 accatgtcca tttccggata cggttttttg aacagaccac attccaggcc cgcgtggata
     1501 atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac cagatgcatc
     1561 accggagaat tagcgtccgg ctgccagcca ggatatgcgc cttttgcttc ggttttcgcg
     1621 ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc gctgtcgatc
     1681 agagaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac cacaccgacg
     1741 ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac accgttcggg
     1801 gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc agctttatcg
     1861 ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag ctcgtttttc
     1921 aggatctcct gataggtatt caccagagat ttcagggcgt cgactttatc agctgcgaca
     1981 gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc gttgaaatca
     2041 ataagacgca gatccagttc ttctgcatga cccgccagga agcgcaccag cagtttgttg
     2101 gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag accttttaag
     2161 gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg caggttggag
     2221 gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg
     2281 ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg
     2341 gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc
     2401 agaaccgcca gcgcagaggc cataccaatg ccgttatccg cacccagtgt ggtgccgcgc
     2461 gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg
     2521 gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac cggtttacga
     2581 ttttccatac ctgctgtagc aggtttacga atcaggatat tacctacctg atcgcgttcg
     2641 acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga
     2701 taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat
     2761 aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca
     2821 ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtttgag
     2881 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     2941 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     3001 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     3061 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     3121 acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg cgttactggc
     3181 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     3241 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     3301 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     3361 gcactttgtc accatctttg caaaaccggc tggtcgtccg ctggttgatg attatgttgt
     3421 tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg tattcgtccc
     3481 gccaatctcc ggtcgctaat cttttcaacg cctggcattg ccgggcgttg ttctttttaa
     3541 cttcaggcag gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     3601 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     3661 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     3721 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     3781 gcgaaatcct cgacgtccag gcgcgtattg tgatgagcga tgccgaacgt accgacgatg
     3841 atttatacga tacggtaatt ggctaccgcg gcggcaactg gatttatgag tgggccaccc
     3901 aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc agtggtcgtc
     3961 actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg aaaggagatg
     4021 acctggccga gcaagcgcag gctttgtcaa accgcgccta tgaagaggcc gctcagcgtc
     4081 tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct
     4141 ttttgcatat gccaaaaggt gatggcccgt ttccaacggt tcttatgtgc ggtggcctgg
     4201 atgcaatgca aacggataac tacagcctgt atgagcgata ttttgcgcca cgtggcattg
     4261 caatgttgac cattgatatg ccctcggtgg ggttttcgtc aaaatggaag ttaacgcagg
     4321 attccagcat gctgcaccag catgtattaa aagcgctgcc taacgtaccg tgggtggatc
     4381 acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg cgtctggcat
     4441 accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt cataccctgt
     4501 tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt ctggccagtc
     4561 gtttggggat gcatgatgct tccgacgaag cgttgcgtgt tgagctgaat cgctactcat
     4621 taaaagtgca aggattgctg gggcgtcgtt gcccaacgcc aatgttatca ggctactgga
     4681 agaacgatcc gtttagcccg gaagaggatt cacgtttaat cacgtcgtca tctgctgatg
     4741 gtaaattatt agagattccg tttaaccccg tgtatcggaa ttttgacaag gcgcttcagg
     4801 aaatcactaa ctggatcgaa aagcgattgt gttaaaaatt tgctaaattt tgtcaatttg
     4861 gtaaaacagt tgcatcacaa caggagatcg caatgacgtt accgagtgga cacccgaaaa
     4921 gcagattgat caaaaaattt accgcactag gcccatatat tcgtgaaggt aagtgcgaag
     4981 ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg gcaccggaag
     5041 tgcgtgagtt ctggggctgg tggatggagc ttgaagcaca ggaatcccgt tttacataca
     5101 gttaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg gtaaaagaca
     5161 ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt ccacgagaag ctgcgtgagc
     5221 tgctgacgac gctgaatctg aagctggaac cggcggatga ttttcgtgat gaaccggtga
     5281 agttaacggc gtgaatgaaa tgtgccggat gcatcacatc cggcaatatt cattaaaatt
     5341 gataggtcat gccaaccgcg acaatatcat cattattgat attcagctta ttatcgctat
     5401 tcagttggtt tattttataa tcaacaaatg ctgacatatt tttgttgaaa taatacgtgg
     5461 caccgacatc aatataattg accagatctt catcaccgat gccctcaata tctttccctt
     5521 tcgataagac ataacccagc gacggacgca gaccaaagtc aaactggtat tgagcaaccg
     5581 cttcaaagtt ctgtgtctta ttagcaaaac caccagatat tggcgtcatt ttacgtgttt
     5641 cagaataaaa agttgccaga taaatattat tggcatcgta tttcagacct gttgcccacg
     5701 cttctgcacg cttacctgtg ccacggcttt gcatgttctg ctcgttggtg cgatcagagt
     5761 tggtataggc accactgata gcgaaatcgc tgccgccaaa gtcatatgtc aatgacgtgc
     5821 cgaagccatc gccgttttgc tttttcacat cgcggttttc gtttttccct tgatattgca
     5881 gggttaagtt cagaccatcg ataacgccga agaagtcggt gttccgatac gtcgccagac
     5941 cgctggcgcg tttggtcata aagttgtcgg tctgcgccga ggagtcgcca ccaaattccg
     6001 ggaacatatc ggtccaggcc tccacgtcat acagcgcgcc caggttacga ccatagtcga
     6061 aagaacccaa atctttatat ttcaatccgg cgaaagcgag acgcgttttt tgctgtgcag
     6121 tatcactctc cgctttattc ccggcaaact ccgcttccca acgaccataa ccagtcagtt
     6181 gatcgttaat ttgtgtttcg cctttaaaac caaaacggat ataactctgg tcgccatctt
     6241 tactgtcatt atcactcata taatgcatgg ctttaacttt gccatagata tccagtttat
     6301 taccgtcttt gttatatatt tctgcggcct gtacggatgc agatgccaca atgcccatca
     6361 ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa cgcgcggata
     6421 ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg ttaaagatat
     6481 attac
//
LOCUS       DAZUGK010000648         2363 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1806T16111
            SAMN43251568-rid23079663.guided.362, whole genome shotgun sequence.
ACCESSION   DAZUGK010000648 DAZUGK010000000
VERSION     DAZUGK010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251568
            Sequence Read Archive: SRR30292672
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349555.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349555.1
            Genome Coverage       :: 117x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,191
            Genes (coding)                    :: 4,967
            CDSs (with protein)               :: 4,967
            Genes (RNA)                       :: 90
            rRNAs                             :: 4, 3 (16S, 23S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 99 of 224
            Pseudo Genes (incomplete)         :: 106 of 224
            Pseudo Genes (internal stop)      :: 65 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251568-rid23079663.guided.362"
                     /serotype="O145:H28"
                     /isolate="1806T16111"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..1079
                     /locus_tag="ACHY5W_005278"
     CDS             120..1079
                     /locus_tag="ACHY5W_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFS4449943.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHY5W_005279"
     CDS             1091..1360
                     /locus_tag="ACHY5W_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFS4449944.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1658..1981)
                     /locus_tag="ACHY5W_005280"
     CDS             complement(1658..1981)
                     /locus_tag="ACHY5W_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFS4449945.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            2225..>2363
                     /locus_tag="ACHY5W_005281"
     CDS             2225..>2363
                     /locus_tag="ACHY5W_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS4449946.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     AL"
BASE COUNT          616 a          454 c          576 g          717 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaatccccct gtgcggaggg gcgactggtg
     1501 tataaggtgt catctctgac gacaacctgg atcgagatac gcgggttcag ggacaccgga
     1561 ctgaactcac cgggaggcac ccggcaccat gttcatggtg atacagaaat gcggcttcag
     1621 cccctctccg gaggggcttt ttatctgaat gattctgtta ttttcgcccg tatgggcata
     1681 tttccagaat gcagcgatga ttaaaacact tatctgtgat atttcctgtg tttgcagggc
     1741 attcctggct gcttttaatt aaattccaga cgtttttatt aaatggggtc acgttgtaaa
     1801 tggtgacggg gagctcttta ttattgagta ttacgcctgt ccgggttggt gtaaaaatac
     1861 agcccggagg gaggaagata tcagactgat accataatat taattttatg ttacaccata
     1921 tggctgaaaa tgatatgtca catgctggct ggattaccgt atcaatcact atccagttca
     1981 tttgctttcc ttattttctt aaggttttaa atgatgccat aaattataag tgatgttttg
     2041 aaagcaatct gtcagcctga gttgtttttc tggtgtcgtt tattctttgt ttaaaataag
     2101 aggacatttt aaattgtcgg cgtttttttg ttatataaga gacatggttt ttttatagcg
     2161 tgttttttat gtgttacatg attgtgtatt ccggataatt actttgctgg cggcaggaga
     2221 ggttatgaca tttaaacact acgatgtggt cagggcggcg tcgccgtcag accttgctga
     2281 tgcacttgcg caaaaaattc gtgaaggatg gcaaccatat ggtgggccgt tttcttcgta
     2341 tacggatgat ggcgcagcac tta
//
